LOCUS       DATMXI010000001         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_65557, whole genome shotgun sequence.
ACCESSION   DATMXI010000001 DATMXI010000000
VERSION     DATMXI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65557"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VN444_00005"
     CDS             complement(<1..969)
                     /locus_tag="VN444_00005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWR20226.1"
                     /translation="MFLLVIAGATIIPERQAIGSELPPSDLRPSSADRARQYFTPEEI
                     DRGRDYARGRYVLYGVRIALTLGLFGLLTLSPLSARIRDLSVSVAAGRVWLAIAVYGL
                     LVALLYYAVTFPVSLYGGFLREHTFGLSRQSVASWAWDYTKGALINAAILLPLLILLY
                     AFIRWDPVRWYLSAWVILVLVMGLLAELSPILLDPLFHTFRPVQDKALVDRLRALIDR
                     AGLTVGPILEIDASQKTAKTNAYFTGLGRTRRIVLYDTLIAGSAPDEVELIVAHELGH
                     WRRHHTWKGMAISATSALAAMWLIARLLNAAADSGRFGFIHPADPMS"
     gene            complement(1025..1510)
                     /locus_tag="VN444_00010"
     CDS             complement(1025..1510)
                     /locus_tag="VN444_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR20227.1"
                     /translation="MPDPARLELAEFYFKEGCRLQASGDLEGAIAAYKRSIDLYPTAE
                     AHTFLGWAYSFQGQIDEAIKECAAAIRVDPDFGNPYNDIGVYLIEKGQYDEAIPWLER
                     AMVAKRYEPRHYPHMNMGRVLMRKGKYQEAIRELKLALDIEPNYTAARVELHKVLGLL
                     N"
     gene            complement(1737..>2737)
                     /locus_tag="VN444_00015"
     CDS             complement(1737..>2737)
                     /locus_tag="VN444_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR20228.1"
                     /translation="VKHMPTAVFDQSRTPESRELLHAFTNSQYFNLDYHVDSYEQITR
                     LIDSSRAKVGIIFPPDYARLLKQQRVTQVQVIVDASDPLVATSAINAANAIGQVGSMR
                     IVSETLQRSTGRATSTASLDVRVRAWYNPDLVSAIFIIPGLLGFILMQTTITIIAMVV
                     VRERERGTLEALIVSPLRRWELMIGKIVPNVLIAYAQMTFALVFGVWIFDIPIRGSLF
                     LLYFLSLFFIMGTLGLGILLSTIARTQQQAMQMSYFIFIPSVYLSGVLFPVEAMPPLA
                     KTVAYMIPLTYYVEIIRGIVLKGIGISYLWTHLLVLTAIGAILITTSILRFHKKLG"
BASE COUNT          628 a          781 c          796 g          532 t
ORIGIN      
        1 cgacataggg tcagccggat gaataaaacc aaagcgtccg gagtcggctg cggcattgag
       61 aaggcgagcg atcaaccaca tggctgccag cgccgaggtc gcgctaatgg ccatcccctt
      121 ccaggtgtgg tgccgtctcc aatggcccag ctcatgcgcc acgatcagct ccacctcatc
      181 aggggcggag ccggcgatca gcgtatcgta caggacgatg cgtctcgttc ggccaagccc
      241 cgtaaagtag gcattggtct tcgcggtttt ttggctggca tctatctcca ggatcggtcc
      301 taccgtaaga ccagcccgat caattaacgc gcggagccgg tccaccaacg ctttatcctg
      361 caccggtcgg aacgtgtgaa acaacggatc gaggaggatc ggagacagtt cagcgagcag
      421 gcccatcaca aggacaagga taacccatgc cgacagatac caacggacgg gatcccatcg
      481 gatgaaagcg tagagcagta tcagcagcgg cagcaggatc gccgcattga tcagtgcccc
      541 tttggtataa tcccacgccc acgaagcgac cgactggcga gagagaccaa atgtatgctc
      601 gcggaggaag cccccgtaga ggctgacagg gaacgtgacc gcataataca gcagagccac
      661 caagaggccg tacaccgcaa tggccagcca cacacgtccg gctgcgaccg agacgctaag
      721 atcgcgaatc ctcgcggaca gtgggctcag ggtcagaagc ccaaagaggc caagggtcaa
      781 cgcgattcgc acaccataca acacgtatcg ccctcgtgcg tagtcccgcc ccctgtcgat
      841 ctcctcaggg gtaaaatact gtcgggccct atcggcggat gaggggcgca aatccgaagg
      901 cggaagctcg cttccaatcg cctgcctttc aggtatgatc gtcgccccgg caatcaccag
      961 taagaacaaa gggatggacc attgcgataa catgcagcgc agacctcgag aaggggtcag
     1021 ccagtcaatt caacagcccc aggaccttgt gcaactcgac gcgggccgca gtataattcg
     1081 gctcgatgtc cagggccagt tttagctctc tgatcgcctc ttggtatttc cctttccgca
     1141 taaggactcg gcccatattc atgtgggggt aatggcgagg ttcgtaccgc ttggccacca
     1201 tggcccgttc gagccacggg atcgcctcgt cgtactgccc cttctcaatc aggtagacac
     1261 cgatgtcgtt atacggattg ccgaaatcag ggtcgacccg gatggcagcc gcgcactcct
     1321 tgatggcctc atcgatctgt ccctgaaagc tgtatgccca ccccaggaag gtgtgggctt
     1381 cggccgtcgg gtagaggtcg atagaccgct tgtaggctgc gattgcgccc tccagatccc
     1441 cactcgcctg caatcggcat ccttctttga aatagaattc cgccaactca agtcttgccg
     1501 gatcaggcat gcttttcacc tatcgttacc ttgttcgcgg gtacagcgag ggccagcctt
     1561 actctataat tgtaacgact taggcattta gactgaaggc tgaaggctat aaattggtgc
     1621 gtgatcacgc atgacgtatc cctaaaagcc ttcagccttc agcctatcca cctgatttac
     1681 tcttccattc taccacgtga acaggccctg ttgcgttctg aaaagggccc ctgtgctcat
     1741 ccgagcttct tgtggaagcg gagaatgctt gtcgtgatca ggatcgcccc gatggcggtg
     1801 aggaccagca ggtgagtcca gagataggag atcccgattc ccttcaggac gatcccgcga
     1861 ataatctcca cgtaataggt gagagggatc atgtaggcga cggtcttggc caagggcggc
     1921 atggcctcga ctgggaagag aacgccggag aggtagaccg acgggatgaa gatgaaatac
     1981 gacatctgca tggcctgctg ctgggtcctg gcgatggtgg ataacaggat accgagacct
     2041 agggtcccca tgatgaagaa cagggacagg aagtagagga ggaacaggct cccacgtatc
     2101 gggatgtcga agatccagac gccgaacacg agggcgaatg tcatttgcgc ataggcgatc
     2161 aggacattcg gaacgatctt cccgatcatc aactcccagc ggcgtagcgg gctcacgatc
     2221 agcgcctcga gcgttccacg ctcccgctcc cgcaccacca ccatggcgat gatggtgata
     2281 gtggtttgca tcaggatgaa gccaagcagg cccgggatga tgaagatagc gctgacaagg
     2341 tccgggttgt accaagcccg cacccggaca tccagggagg cggtggaggt ggctcgaccg
     2401 gtgctccgct gcagcgtctc cgatacgatc cgcatcgagc caacctggcc gatcgcgtta
     2461 gccgcgttga tggccgaagt cgccaccaat gggtccgagg cgtccacgat cacctggacc
     2521 tgcgtgacgc gctgctgctt gagcaagcgg gcatagtcag gtggaaagat gattcctacc
     2581 ttggccctgc tgctgtcgat gaggcgtgtg atctgttcat agctgtccac atgataatcg
     2641 aggttgaaat attgagagtt ggtaaacgca tggaggagtt ctcgactctc aggcgtccgg
     2701 gactgatcga agacagcggt cggcatatgc ttgacgt
//
LOCUS       DATMXI010000002         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_65014, whole genome shotgun sequence.
ACCESSION   DATMXI010000002 DATMXI010000000
VERSION     DATMXI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65014"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VN444_00020"
     CDS             complement(<1..1355)
                     /locus_tag="VN444_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWR20229.1"
                     /translation="MDSTGNRSDDPTRPQEPTRMTRMRKVRFEPLGITIECDGTESVL
                     QYALRQGLRLVDYRCADGECGGCKARIRSGQAHLVDPPDHVLSASEQAQGHVLLCRTH
                     VQSDLVIELLAGSLLAPPLPDVQPARSAEALLGPLAATAELLSDGLVVLDAQGQLLLC
                     NRAAAHLLQLDPARPSVAAIPELSEIGRTLLTRAPSSPQEVKVYCGRSGRLLTAWGVA
                     LRHGPQVVAAALLFPTAARLEPPARKPKAIVVGAKYTFSDLVGQSAAFRNATKIGLIA
                     AGNTLSVLISGESGTGKEMLAHAIHASSPRAPKPFIAVNCGAIPRELIESELFGYEEG
                     TFTGARKGGRLGRFEEAQGGTLFLDEVSECSPSTQVALLRVLEQQELTRLGSGQPVPL
                     DVRIIAATNKDLLKEVAKGLFREDLYYRLNVISIHLPPLRERREDIPLLAASFLADVA
                     AA"
     gene            1590..1970
                     /locus_tag="VN444_00025"
                     /pseudo
     CDS             1590..1970
                     /locus_tag="VN444_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1991..3373
                     /locus_tag="VN444_00030"
     CDS             1991..3373
                     /locus_tag="VN444_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR20230.1"
                     /translation="MPKLGRVLVIDDEVDMLENCARLLTRFGYEAMTEAESGKAAALY
                     ERERPDLVLTDLRMPGHDGLGVLQAIRAIDPEATVILITAFATIETAVEAIKEGAFDY
                     LPKPFSADQLRICVERAMDQRRLREENRRLLEQLTETHRFDNIIGRSLPMLRVFETIK
                     KVAKSEANILIIGETGTGKELIARSLHVNSRRATGPFIPVDCVSLPENLLESELFGHE
                     KGAFTGAQATRPGLFEFASGGTIFLDEVGDISLNLQAKLLRVLQERQVRRVGSNRMIE
                     VDVRVISATNHDLAQAVAMGRFREDLYYRLNVISLPLPPLRDRAGDIQLIAHHYLTKY
                     AAGSEKEVKGVTPEAMRLLEAHNWPGNVRELQNVMERAVVLAEHELVRPQDLPEQIRI
                     KTEAALLMSPNELPLKRAKEVWAGSFERDYLIQLLKRHDGNISQAAKAACVDRKTVHR
                     LIKKYGIKPS"
     gene            3613..4176
                     /locus_tag="VN444_00035"
     CDS             3613..4176
                     /locus_tag="VN444_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HWR20231.1"
                     /translation="MTGRPGVGKTTAIHRIAASLVGWRLAGFYTEEIRESGQRQGFRA
                     VSFDGSEKVMAHIDIASRYRVGRYGVDLSVIDQLADLTLDLIPEVDCYLVDEIGKMEC
                     CSQRFIAAARRLLTSHCLVVATIALRGGGFIAEVKQRTDVEQWEVTLKNREELPERAL
                     AWLRAARGPIPVESGHEPGMSPDRLQP"
BASE COUNT          892 a         1262 c         1486 g          799 t
ORIGIN      
        1 gcggccgcga catcggccag gaaggaggcc gccagcaggg ggatatcctc gcgccgctcc
       61 cgcaagggcg gcaggtggat cgaaatgaca ttgagccggt agtacagatc ctcccggaac
      121 agccccttgg cgacctcttt aagcaggtct ttattggtgg cggcgatgat ccggacatcg
      181 agggggaccg gctggccgct ccccagtcgg gtgagctcct gttgttccag cacccgcagg
      241 agcgccacct gggtgctggg cgagcactcg ctgacctcat cgaggaagag ggtgcccccc
      301 tgggcctctt cgaaccggcc caaccggccg cctttgcgcg ccccggtgaa ggtgccctct
      361 tcatagccga acagctccga ctcaatcagc tcccggggga tggcgccgca gttgacggcg
      421 atgaacggct tgggggcgcg gggggagctg gcgtggatcg catgggcgag catctcttta
      481 ccggtccccg actcccccga gatcaggacc gagagggtgt tgccggcggc gatgaggccg
      541 atcttggtgg cgttgcggaa ggcagcggac tggccgacca gatcactaaa ggtgtacttg
      601 gcccccacca cgatcgcctt cggtttgcga gccggcggct ccagccgggc ggcggtcgga
      661 aagagcaggg cggcggccac cacctgtggg ccgtgccgca gtgcgacgcc ccaagcggtg
      721 aggagacggc cgctgcgccc gcagtagacc ttgacctcct gggggctgga gggcgcgcgg
      781 gtcagcaggg tgcggccgat ttcggacagc tccgggatcg ccgccacgga gggccgggcg
      841 gggtcgagtt gcagcaggtg tgcggccgcc cgattgcaca gcagcagttg cccctgcgcg
      901 tccaggacca ccaggccgtc gctgagcagt tccgcggtag cggccagcgg ccccagcagc
      961 gcctcggcag agcgcgccgg ctggacgtcg ggcagcggcg gggcgaggag tgagcccgcc
     1021 agcagctcga tcaccagatc gctttggacg tgggtgcggc agagcaggac gtggccctgg
     1081 gcctgttcac tggcggacag gacgtggtcc ggggggtcga ccaggtgggc ctggccgctg
     1141 cggatgcggg ccttacaccc gccgcactcg ccatcggcgc agcggtaatc gaccaggcgg
     1201 aggccctgcc gcagggcgta ctgcaggacc gactcggtgc cgtcacactc gatggtgata
     1261 cccagcggtt caaaccggac cttgcgcatc ctcgtcatcc gtgtgggctc ctggggacgg
     1321 gtcgggtcgt ccgatcggtt gccggtgctg tccatacccc tagcaactct tgggccaggt
     1381 gggggcagcc ggatggaacg gatctgattc tctgatgaca gaggaagggt acggaggttg
     1441 acccgcgtgc tccgaacaga ctaagatagt taaaattgaa tagatgaaat tatgatatga
     1501 gtagtggtgt cgaggtaaca gagaaagtgt caacgaaaag cgacgacaat atcctggctc
     1561 tcctgacctg gggacatcgc cagtccccga tgtacgatct ggagcggcgc ctcgctcctt
     1621 cgctaccgaa ggtgaaggga aacgtcagtc aactggagga ggtattgctg aatctgttca
     1681 ccaatgctcg cgaggcgatg actgccggcg gccgcctccg tgtggagagc cgcttaggag
     1741 cagacgacag aactgtcgag atcctggtcg ccgataccgg ggatggcgtc cccgagatgc
     1801 ttgtccagta gatctttgac ccattcttca ccacgaagca gaacggtaca gggctgggcc
     1861 tgtcgatcag ttatggaatc gtcaaagagc atggggggcg gttggacgtc cggagcgaga
     1921 ctgggaatgg gactaccttt atcatgacgt tgccggcgga ggacgcgtga atgatgagag
     1981 aagggacagg atgccgaagc taggccgagt actggtgatc gatgacgaag tggacatgct
     2041 cgagaactgc gccaggctgc tgactcgctt tggctacgag gcgatgaccg aagccgagag
     2101 cggcaaggca gccgcgctgt acgagcggga aaggccggat ctggtgctca ccgatcttcg
     2161 tatgccgggc catgacggcc tgggcgtcct tcaggcgatc cgggcgatcg atcccgaagc
     2221 gaccgtaatc ctgatcaccg cctttgccac gatcgagacc gccgtcgagg cgatcaaaga
     2281 aggcgcgttc gattacctgc ccaagccgtt ttcggccgat cagctcagga tctgtgtcga
     2341 gcgggcgatg gaccagcgtc gtttgcggga agagaaccgg cgacttctgg agcaacttac
     2401 cgagacccat cggttcgaca atattatcgg caggagcctg ccgatgcttc gggtctttga
     2461 gaccattaag aaggtggcga agagcgaggc caacatcctg atcatcggcg agaccgggac
     2521 cggcaaagag ctgattgcca ggagtctcca tgtcaacagc cgccgggcga ctggtccgtt
     2581 tatccccgtt gattgtgtct cgcttccgga gaacctgttg gagagtgaac tgttcggaca
     2641 cgagaagggc gcgttcacgg gagcgcaggc gactcgtccc gggctgtttg aatttgccag
     2701 cggtgggacc atcttcctgg atgaagtggg cgacatcagc ctgaatcttc aggcgaagct
     2761 gctgagggtc ctgcaggaac gtcaggtgcg acgggtcggc tcaaaccgga tgatcgaggt
     2821 agatgtacgc gtcatctcgg ctaccaacca cgacctggcc caggccgtcg ccatgggcag
     2881 atttcgggaa gacctctact atcgcctgaa cgtcatctcg cttccgctgc cgcccctgcg
     2941 cgatcgcgcg ggagatatcc agttaatcgc gcatcactac ctgacaaaat atgcggccgg
     3001 cagcgagaag gaagtcaaag gcgtcacgcc ggaggcgatg cggctcctgg aggcgcacaa
     3061 ctggcctggg aacgttcgcg aacttcagaa tgtcatggag cgagcggtgg tcctggccga
     3121 acacgagttg gtccggccgc aggatctacc ggaacagatc cgcataaaga ccgaagctgc
     3181 cttgctgatg tcgccgaatg aactgccgct caagcgggca aaagaggtgt gggccggctc
     3241 atttgagcgg gactatctga tccagctcct caagcggcat gacggcaata tctcgcaggc
     3301 cgccaaggcc gcctgcgtgg accggaaaac ggttcatcgc ctgataaaga agtacgggat
     3361 caagccctcg tagcggcctg gcagatagga ccgccagtgc agcgtatcta acgcttcttt
     3421 agattcattc aggagccgtc attctcgcag aagcgggaat ccagcgccga cggactggat
     3481 tccgggtcaa gcccggaatg acgatcacca cgaagggtct catcggacac tgcactaacc
     3541 gtcattcttc tctatccata ttacgaccgc gacggggata tggtgaactc atccaacaga
     3601 catgtgctgc ttttgacggg tcgtcccggg gtcggcaaga ccacggcgat acaccggatc
     3661 gccgcctccc tcgtaggatg gcggcttgcc ggcttttaca ctgaggagat ccgcgaatcc
     3721 gggcagcgcc aggggttccg cgcggttagc tttgatggct ccgaaaaggt tatggcccac
     3781 atcgacattg cgagtcgcta tcgcgtggga aggtacgggg tagacctgtc ggtcatcgat
     3841 cagcttgccg acctcacgct ggacctgata ccggaggttg attgctatct tgtcgatgag
     3901 atcggcaaga tggagtgttg ctcgcaacga ttcatcgccg ccgcacgaag actcctcaca
     3961 tcccattgtc tcgtggtcgc gacgatcgcc cttcggggcg gcggcttcat cgcagaagta
     4021 aagcagagga ctgatgtcga gcagtgggag gtgacgctca agaaccggga ggagctgccc
     4081 gagcgtgccc tcgcgtggct tcgagccgcg cgcgggccaa tcccggttga gtcagggcat
     4141 gagccgggaa tgagcccaga tcgtctgcag ccctgatagg gtcagactga cagtcgctgc
     4201 agtgtttgcc ggccacgggc ggtactggag gaacttgaca agcccttgga aaggcaggac
     4261 aatcgccgca gttgttgcag gtttccgggg tgggagatat tggtcagcga actcaataga
     4321 tgggtgtcca gattgcccag aaaagccacg gacaatttcc gtagcgagaa tcataataga
     4381 ggatagagtt cctggaagcg ggaatggttc cctcaataga gggaacatga gtctccgag
//
LOCUS       DATMXI010000003         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_284642, whole genome shotgun sequence.
ACCESSION   DATMXI010000003 DATMXI010000000
VERSION     DATMXI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284642"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..766)
                     /locus_tag="VN444_00040"
     CDS             complement(317..766)
                     /locus_tag="VN444_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HWR20232.1"
                     /translation="MAYEELRERGLVEEVRPDFRQVLTLLTRAMKDLSTAKANVSIDK
                     EWAYTIAYQGMLGAAKALVTAEGWRPKGRDQARTVIILVGELLGEEGRSLVNGFDRMR
                     RKRQDFMEEPQTPIPRYEVEGALKDAQTLIERLVDLAREKNPQLAFL"
     gene            complement(756..1163)
                     /locus_tag="VN444_00045"
     CDS             complement(756..1163)
                     /locus_tag="VN444_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20233.1"
                     /translation="MASEKVGNLRYYRVNKASPIYTEIKGVVAKTTGIHASLREGLRK
                     IKGIQTAMVYGAEQHEAEEGLGPVRLMVIGQVDLNDLNDTIRGLENRLNREINYLVFD
                     GAEYQRRKMEDDPFLVEVLKGRRTILIGTDDGL"
     gene            complement(1531..2085)
                     /gene="dcd"
                     /locus_tag="VN444_00050"
     CDS             complement(1531..2085)
                     /gene="dcd"
                     /locus_tag="VN444_00050"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWR20234.1"
                     /translation="MSIKSDKWIKRVAVEHQMTVPFEEGQVRNGVISYGLSSYGYDIR
                     VANEFKIFTNVNTTIVDPKNFDERSFVDFKGDVCIVPPNSFALARTVEHFRIPRNVLT
                     ICLGKSTYARCGVIVNVTPFEPEWEGFATLEISNTTPLPAKIYANEGIAQVLFFESDE
                     PCLVSYADKKGKYQAQQDITLPKI"
     gene            complement(2161..>2992)
                     /locus_tag="VN444_00055"
     CDS             complement(2161..>2992)
                     /locus_tag="VN444_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside reductase class II"
                     /protein_id="HWR20235.1"
                     /translation="VRMQAAFQKQTDNGVSKTINLSNSATPEEIAKAYMMAYELGCLG
                     ITVFRDGCKGTQVLHLGTGAKPALSDTEEPALSPAEEPALNDAEVVTKREKTPQASPI
                     VMKMGREAALKVKPRPRTVKGVTYRAETPLGTAFVTVNQNGEGEPFEVFASVGKAGSD
                     TSAVSEAIGRLISMILRLPSSMSPRERVEQIVSQLSGIGGRRPMGFGKDRVRSLPDAI
                     AQVLSEHIGLTEPGVQEMQAALGKTTQKVGDMCPDCGSATLVYEEGCQKCYSCGFSEC
                     "
BASE COUNT          608 a          931 c          725 g          728 t
ORIGIN      
        1 gcttcatctt cggctcctcg ggatcgatgt cggtcaccac ggtcttgcag tgttcctatt
       61 tcttcttggc tgccttcttt ggtgctgcct ttgttgctgc cttcttcttc gctgccattg
      121 aggttctcac cccctttcgt ggttagggcc tcccacactg ctcaacgagg ctaacaaccc
      181 ctccccgtct ttttcgccgc ctttggtgct gccttctttg gtgctgcttt ctttggtgct
      241 gcttttggtg caggcttctt cgccgccatt cagtctctca ccccctttct acgctatcgg
      301 tccatcctca gcctcgctac aggaacgcta actgcggatt tttctcgcgg gcgagatcca
      361 cgagcctttc aatgagggtc tgtgcgtctt tcagcgcccc ctcgacctca taccgaggga
      421 tcggagtctg gggttcctcc ataaagtcct gccgtttgcg acgcatccgg tcgaagccat
      481 taaccagaga tcgcccctct tcacccaaaa gctcccccac tagaatgata accgttctgg
      541 cctggtcacg accctttggg cgccaccctt cagccgtcac taacgccttg gcggccccca
      601 gcattccctg gtaggcgatg gtataggccc actccttgtc gatgctcaca ttggctttgg
      661 ccgtcgacag atctttcatg gcccttgtca acaacgtcaa gacctggcgg aagtcaggtc
      721 tgacctcctc gaccaagccg cgctcccgaa gctcctcata agccatcgtc cgttccgatc
      781 aggatcgtcc ttcggccctt gagaacttca acgaggaacg ggtcatcctc catctttctc
      841 cgctgatatt cggccccatc gaacaccaga tagttaatct cgcgatttaa ccggttctcc
      901 agtcccctga tggtatcgtt aagatcgttc agatcaacct gtccaatgac catcagccga
      961 acagggccga gaccctcttc ggcctcgtgc tgctctgccc catagaccat agccgtctgg
     1021 atccctttaa tttttcggag tccttctcgg agactcgcat gaataccggt agtcttggcg
     1081 acgaccccct tgatctcagt ataaatgggc gacgccttat ttacccggta gtaccgaaga
     1141 ttcccgacct tttcgctagc caacaaacca gccctctcca gattatctaa ctcccgcttg
     1201 accccggaga tgtcccgtcc aaggtgtcgc gccagttctc tgacgtaaaa gcgcttatcc
     1261 aacctagtga aaagaaatgt gagaagactc acacgtgttt ctgatgaaaa caatcggatc
     1321 agcaaccgca cacctcatcg ttgttgatat ctttacaacg accgttctgt ctgtgtcaac
     1381 aataatcgta caataagtca aacttttttt catgacgaaa tcgtcgacca caagagagac
     1441 agcgactgcc ttaggaatgg ttgcgcatac ttgcgaatcg cgctgtcact cttcagtaaa
     1501 atggagtgag aaaatgagga aaggttgtgg ctagatttta ggcagcgtga tatcttgctg
     1561 ggcctggtat ttccccttct tatccgcata cgacacgagg cacggctcat cgctctcgaa
     1621 aaagaggact tgcgcgatcc cttcgttggc atagatcttg gcgggtaatg gtgtggtatt
     1681 gctaatctcg agggtcgcaa acccctccca ctccggctcg aacggggtga cattcacgat
     1741 gacaccacaa cgcgcatacg tcgatttccc caaacaaatt gtcaggacat tccgaggaat
     1801 cctgaaatgc tcgacggttc gggcgagggc aaaggagttg ggcgggacaa tgcagacatc
     1861 gcccttgaaa tctacgaacg accgctcgtc gaagttcttt gggtctacga tggttgtgtt
     1921 gacattagta aagatcttaa actcgttcgc cactcggatg tcatacccat acgacgagag
     1981 accataggag attaccccgt tccggacctg cccctcctcg aacggaacgg tcatctgatg
     2041 ctccaccgcc acccgcttaa tccacttatc tgatttaatc gacatatgac gtcctcccac
     2101 gagacctcaa gtctccactt ttacgaaccg ttaagattgc gcagatatgc gacatgcggc
     2161 ctagcactca ctaaacccgc aggagtagca cttctggcaa ccctcttcgt agacgagggt
     2221 agccgaacca cagtcagggc acatgtcgcc tactttctgg gtcgtcttgc caagagccgc
     2281 ctgcatctct tgaacgccgg gctcggtcag accgatatgc tcggacagga cctgggcgat
     2341 ggcatccggc aacgaccgga cccgatcctt accgaagccc atcggccgac ggccgccgat
     2401 gccggagagt tggctcacga tctgctcgac ccgctccctg ggcgacatcg atgacggcag
     2461 cctgaggatc atagagatga gtcggccgat cgcctcggac accgcagagg tatcgctccc
     2521 ggccttcccg acgctggcga agacttcaaa cggttcacct tccccgttct ggttgacggt
     2581 gacgaaggcg gtccccagcg gcgtctccgc cctatacgtg acgcccttca cggtccgcgg
     2641 tctcggcttg accttgagcg ctgcctctcg gcccatcttc ataacgattg ggctcgcttg
     2701 cggcgtcttc tcccgctttg tcaccacttc agcatcgttc agggcaggct cttcggctgg
     2761 actcagcgca ggctcttcag tatcgctcaa ggcaggctta gcgccggtgc cgagatgcaa
     2821 gacctgggtg cccttacagc catcgcggaa gaccgtaatc ccgaggcatc caagctcata
     2881 cgccatcata taggccttgg cgatctcctc cggcgtcgcc gagttggaca ggttaatcgt
     2941 cttagagacg ccattatcgg tctgcttctg aaaggctgcc tgcatcctga cg
//
LOCUS       DATMXI010000004         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_87593, whole genome shotgun sequence.
ACCESSION   DATMXI010000004 DATMXI010000000
VERSION     DATMXI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87593"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VN444_00060"
     CDS             <1..831
                     /locus_tag="VN444_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWR20236.1"
                     /translation="ALAGAIVLGPRIGKFNKDGTPNAIPGHHIPMAMVGTFILAFGWF
                     GFNAGSTLAGTDLRIAVVATNTMLAGSAGALTATFFMWFKYGKPDPSMVANGLLAGLV
                     AITAPCAFVNSVSAFLIGAIAGVLVCLSVFFVERTLKIDDPVGAVSVHGVCGAFGVLS
                     LGLFADGVYGDGWNGVPGGVRGLFYGDASQFIAQSVGTLTCIVFVFTVSFAFFKILDA
                     VMGLRVSADVEIEGLDVPEVGALAYPDFTVLPAHHIPAGQPATHSFTPSMVETVKGMS
                     "
     gene            884..1474
                     /locus_tag="VN444_00065"
     CDS             884..1474
                     /locus_tag="VN444_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWR20237.1"
                     /translation="MMPKKCAVIIDDSLGSRATLGQALKRLGFEVVGEGASGSEAIRL
                     AQALKPNVLFLAVGLPDLDGLTTSAQILETHALPIIILSSHLNPEVIQRAKEAGVMAY
                     LVKPLREEELLPAMELAISRFEEFNALRKENADLKRILEDRKLIERAKGILMEREKIS
                     EPQAFGRIQKASMNSRRSMAEIAQAILLSEAVSTRT"
     gene            1559..2020
                     /locus_tag="VN444_00070"
     CDS             1559..2020
                     /locus_tag="VN444_00070"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWR20238.1"
                     /translation="MKGVIHPLSVHLPIALLLMAFMTMGYWLFKGLATSVFENRIYSL
                     TRFNTAAGLLFVLLSIATGIRDVQAGYWIAFNSPLGKWLYVKIILALLMVIIYSLFLW
                     QSRKKPQYLQEDPKIMTWCLTTQMLGFVLVITLTALGTMLVFYPHLLAQLG"
     gene            2059..>2697
                     /locus_tag="VN444_00075"
     CDS             2059..>2697
                     /locus_tag="VN444_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR20239.1"
                     /translation="MTRTPAKIVILGGGFGGLYAAMTLQRDLAGTNLAQVTLVDRRNY
                     FTFTPFLPEVAAGTLGRAHVTYPFRFLAQKEQFYFIQGAVQDFDLVKRTVRTETATIP
                     YDYLIVSLGGAPAFVDNPQIETHALTLHSVDKALAIRNHIIRLFEQAVVERDPIRRRQ
                     LLTLVVAGAGPCGVEVAAELHHLIRTALLKYYPVDPSEIRILLVSRGDRILPH"
BASE COUNT          523 a          775 c          790 g          609 t
ORIGIN      
        1 gccctggccg gcgccatcgt cctcggtccc cgcatcggga agttcaacaa ggacgggaca
       61 cccaacgcga tccccggcca tcatatcccc atggcgatgg tcggcacctt catcctggcc
      121 ttcgggtggt tcggctttaa cgcaggcagt accctcgccg gaactgattt gcgcatcgcg
      181 gtggttgcga ccaacaccat gttggccggc tcagcggggg ccttgaccgc caccttcttt
      241 atgtggttca agtatggcaa gcctgatccc agcatggtgg ccaatggatt gttggcgggc
      301 ctcgtagcga ttacggcccc ctgtgcgttc gtgaattcgg tctcggcctt ccttattggt
      361 gccattgccg gggtgctggt ctgtttgagc gtcttcttcg tcgagcggac cttgaagatc
      421 gatgaccctg tcggcgctgt gtcggttcac ggtgtctgcg gcgcctttgg cgtcctgagc
      481 ctcggcctgt tcgctgacgg tgtctatggc gacgggtgga acggtgtgcc cggaggggtc
      541 cggggcctct tttatggcga tgccagtcaa ttcatcgccc aatcggtcgg taccctgacc
      601 tgcatcgtct ttgtctttac tgtgtccttt gcgttcttca agattctcga cgctgtgatg
      661 gggcttcgtg tttccgcaga cgtggagatc gaagggctcg atgtgcctga agtaggcgcc
      721 ttggcgtatc ccgactttac ggtccttcca gcacaccaca tcccggccgg ccagccagcc
      781 acacactcgt ttacaccgag tatggtcgag acagtaaagg gaatgtcgta acgtcttcca
      841 tggtggcctt ttattgctgc tacttaacga gcgtggtcgc gggatgatgc ccaaaaagtg
      901 cgcggtaatc attgatgaca gccttggatc gcgggcgaca ctcggccaag cactgaagcg
      961 gcttggattt gaggtggtag gagaaggggc gagcggctcc gaggcgattc gcctggccca
     1021 agcactgaag cccaatgtgc tcttcttggc ggtgggtctc ccggacttgg atggcctgac
     1081 cacatccgct cagatcctgg agacccatgc cctccccatc atcatcctga gcagccacct
     1141 caacccggag gtgatccagc gggccaaaga ggctggcgtg atggcctatc tggtgaagcc
     1201 gctacgcgaa gaggagctgt tgccggcgat ggaactcgcc atctcccggt tcgaggagtt
     1261 caacgccctc aggaaagaga atgcagactt gaagcggatc cttgaagacc ggaagctgat
     1321 cgaacgcgca aaagggatcc tcatggaacg agagaagatc tcggaaccgc aggcattcgg
     1381 acggattcaa aaagccagca tgaactcacg gcgatcgatg gccgagatcg ctcaagccat
     1441 cttgctgagc gaagcggtct ctaccaggac gtaataaaga gcgagggcga gggtcggtcc
     1501 gggatcagga tcctcaggat gtgtgattgt tgtatcgtgt aaggaagtgc gacgcgagat
     1561 gaaaggggtc attcatcctc tctccgtgca tcttcccatc gccctgctgc tgatggcctt
     1621 tatgacaatg ggctattggc tcttcaaggg gctggccacg tcggtcttcg aaaaccggat
     1681 ctacagcctc acgcgattca ataccgccgc aggattgctg ttcgtcctcc tgtccatcgc
     1741 gacagggatc cgggatgtgc aggcgggtta ttggatcgcc ttcaactccc ctctgggcaa
     1801 gtggctctac gtgaagatca tcctggctct gctcatggtg atcatctata gcctattcct
     1861 ctggcaaagc cgcaagaagc ctcagtatct ccaagaggac ccaaagatta tgacgtggtg
     1921 cctaacgaca cagatgctgg gcttcgtcct ggtgataact ctcaccgcgc tcggaacgat
     1981 gctggtgttc tatccccatc tccttgcgca actcggctga ggagatgaga cgaaccggcg
     2041 gatgaaagga atggtcgcat gactcgaacg cccgcaaaga tcgtcattct cggaggagga
     2101 ttcggcggcc tgtacgcggc catgacgctt cagagggatt tggctggaac gaacttggct
     2161 caggtcaccc ttgtggaccg gcgcaactat tttaccttca ctccgttcct gcctgaggtg
     2221 gctgcaggca ccctcggccg ggcgcatgtg acctacccgt tccgattcct tgcccagaaa
     2281 gaacagtttt actttattca aggggcggtg caggactttg accttgtgaa gcgcacggtc
     2341 cgtaccgaga ccgcgaccat tccctacgat tatctgattg tgtccttggg cggcgcgccc
     2401 gccttcgttg acaaccctca aatcgagact cacgcgctga cgttacactc tgtcgacaaa
     2461 gccttggcca tccggaacca tatcatcaga ctgtttgaac aagcagtggt cgagcgggat
     2521 ccgattcgcc ggcgtcagct cctcaccttg gtagtggccg gagccggacc ctgcggggtc
     2581 gaggtagccg ctgagttaca tcacctgatc cggacagcct tgctcaaata ctatccggtg
     2641 gacccatctg agatcagaat cctgttggtc tcgaggggcg atcggattct cccccac
//
LOCUS       DATMXI010000005         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_19662, whole genome shotgun sequence.
ACCESSION   DATMXI010000005 DATMXI010000000
VERSION     DATMXI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19662"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VN444_00080"
     CDS             <1..654
                     /locus_tag="VN444_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWR20240.1"
                     /translation="TLQGGGATDLPYTGNQFPSVDGVQGETLILGHRATFFYDTRDNL
                     ISPTDGMAVTAYAELNQNIRNGDHPVYSRYELEIKKLLPSESKHAILIMRADLQATIG
                     DQVPFFEQSSLGGQNNLRGYGVDRFIDKNLVALSIEERIHLARAKVAGVMADFEIAPF
                     LDTGQVFNDFKDVSFKDYRLTPGVGFRGIVRPNVVGRVDYGYSQEGGAVFAGLDFPY"
     gene            629..1684
                     /locus_tag="VN444_00085"
     CDS             629..1684
                     /locus_tag="VN444_00085"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HWR20241.1"
                     /translation="MPDSTSHTDPSSRGIIGRAIFGLALLVWLLSFVCAQPSPVKAEG
                     FGPFPVRNFQPFQQLVLTVPGDRAAVVKQGTLDVRLELAETASIYDDNSPPMNVTVKF
                     ETLRSGLFLRYGATDRLELGLEVPVLYRYDGFMNGAIKGVERATTGLNPDQAALENTS
                     YVFNVTRNGQTIMSGGPGATGLGDTTLMSKYQFLKEGAAMPAVSLRGAVKLPTGDQSA
                     FFGSGSPDFGLGLAAEKLVAGRWILYGNVTGVVPTGTIAGFGLHPTLSGLAAIEYLWS
                     ENLSVTAHFNYYSSPFSGTGSNVFDKDVTESVLGFSYRVAQPLLWQVYAVENLDFIRG
                     SAADFTLSTVLTYRFES"
     gene            1861..>3528
                     /gene="dxs"
                     /locus_tag="VN444_00090"
     CDS             1861..>3528
                     /gene="dxs"
                     /locus_tag="VN444_00090"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWR20242.1"
                     /translation="MSILKTIHSPADLKRLSPTQFPALCQELREQIIGVVSNVGGHLA
                     SNLGVIELTVALHSLLNTPKDKIVWDTSNQAYAHKLLTGRREGFHTLRQYGGLSGFCK
                     REESEYDTFNAGHAGTGVSAAYGMVEARDQLKQRHKVVCIVGDGAMTAGMTLEGLQHA
                     GGLGKDFLVILNDNQMSISKNVGAISSYLNRTITGDFYDKMREETGQLLGKIPHIGQD
                     VQKWARRAEELAKGAILPGLLFEELGFRYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VVTKKGLGYEPSVKNPVWFHACPPFVRETGVPAKKAARPSYTQIAIESLVKLARADKR
                     IVAITAAMCEGTGLNIFEKEFPDRLYDVGIAEQHAVTFAAGLATQGLRPVVALYATFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDYAFLRHIPNMVVMAPKDE
                     NELQHMMKTCVLHDGPASVRYPRGLSLGVAMDKEPEALPVGKGELLRDGTEVAIVAIG
                     VPVSHAVTAAKRLEEEGISAAVINARFVKPLDQDLIVELAKRVRYVVT"
BASE COUNT          764 a          884 c         1071 g          809 t
ORIGIN      
        1 actctgcagg ggggtggtgc aacggatctt ccctatacgg gcaaccagtt cccttccgtc
       61 gacggcgttc aaggcgaaac cctcattctc ggacaccggg cgacgttctt ttacgatacg
      121 cgcgacaatc ttatctcgcc gaccgatggg atggccgtga cggcctatgc agaactgaac
      181 caaaatatcc gcaacggcga tcatcccgtc tactcccgtt atgagctcga gatcaagaag
      241 ctgctaccga gtgagtcgaa acacgcgatt ctcatcatgc gggcggatct tcaggctacg
      301 attggggacc aagtaccgtt cttcgagcaa agctccttgg gagggcaaaa caatttgcga
      361 ggctatggtg tcgatcgctt catcgacaag aatcttgtcg cgctgagtat cgaagagcgt
      421 attcatctgg cacgagcgaa ggtggcgggg gtgatggcgg actttgagat tgcgccgttt
      481 ctggatacgg gacaggtgtt caatgatttc aaagacgtga gttttaagga ttaccgtttg
      541 acacccggtg tagggttccg cgggattgtt aggcccaacg tcgtcggacg cgttgactac
      601 ggctatagtc aggagggtgg agcggtcttt gccggactcg acttcccata ctgatccatc
      661 gtcgcgaggt atcattggtc gcgcgatatt tggtctggcg ctgctcgtct ggctcctctc
      721 cttcgtctgt gcccagccat cgccggtcaa ggcggagggg ttcggtccgt ttcctgtcag
      781 aaatttccag ccctttcagc agctggtgtt gactgttcca ggcgaccgcg ccgccgtcgt
      841 gaaacagggt actctggacg tacggttgga acttgcggag acggccagta tctacgacga
      901 taattcgccg ccgatgaacg tgactgtcaa attcgaaact ctcaggtccg gcctctttct
      961 tcgttatggt gccacggaca gattggaact cgggctagag gtgccggttc tctatcggta
     1021 cgatggattt atgaatggag ccattaaggg ggtcgagcgg gccacgactg gcttgaatcc
     1081 ggaccaagct gcgcttgaga acaccagcta cgtgttcaat gtcactcgga acggtcaaac
     1141 aattatgagc ggaggtcctg gtgcgacagg gttgggggat acgacgctga tgagcaaata
     1201 tcagttcctg aaggaggggg cggcgatgcc ggctgtttcc ctccgtggcg cggtgaagtt
     1261 gccgacgggt gatcaatcgg cttttttcgg aagcggaagt cctgatttcg gattgggtct
     1321 cgcggctgaa aaacttgtgg ctggccgatg gatactctat ggtaacgtga ccggagttgt
     1381 cccgaccgga accatagcag gttttggcct gcaccctacc ctgtccgggc ttgcggccat
     1441 cgaatatctc tggtcagaga atctgtcggt cacagcccat tttaactact attcatcgcc
     1501 attttccggg accggctcga atgtattcga caaggatgtg acagaatcag tcctagggtt
     1561 cagctatcgc gtagcccagc ctctgttgtg gcaagtctat gcagtcgaaa atctcgactt
     1621 cattaggggg agcgcagccg acttcacgct ctcgacggtg ttgacctatc gatttgagtc
     1681 atgagcgata cggaagattc ggtggtgaga gaagctaaca ccttgacaac atgggactct
     1741 catgtgaaac tcatccctgc gagcgtccaa actgccacac cgctttgcca gtgatcatca
     1801 gggcttagct tgttgttaca ggggtatgtg cgtacgcatt gacgcaaagg agcatccgct
     1861 atgtcgattt tgaagaccat tcacagtccg gctgatctga aacggctatc gccaacgcag
     1921 tttccagcct tgtgccagga gctgcgggaa cagattatcg gcgtggtatc caatgttgga
     1981 ggtcacctgg cctcgaacct tggagtcatt gaactgactg tggccctaca ttctctgctc
     2041 aacaccccca aagataagat cgtgtgggac acgagcaatc aagcctatgc acacaagctg
     2101 ctgacgggac gccgcgaggg gttccatacg ctcaggcaat acggagggtt gagcggtttt
     2161 tgtaagcgtg aggaaagtga atacgatacg ttcaatgcag gccatgcggg aaccggcgta
     2221 tccgctgcct atggcatggt cgaggcgcgt gaccagttga aacagcgaca caaggtcgtc
     2281 tgcattgttg gtgacggggc gatgacggcc gggatgacgt tggaaggttt gcagcatgcc
     2341 ggagggctgg gaaaggattt cctggtcatt ctgaacgata atcaaatgtc catttcgaag
     2401 aacgtcggag ccatttcctc ctacttaaac cggaccatca ccggagactt ttatgacaag
     2461 atgcgggagg agacagggca actgctgggg aaaatccccc atatcggtca ggacgtgcaa
     2521 aagtgggccc gccgggctga agagttggcc aagggagcaa tccttccagg gctgctcttc
     2581 gaagagctcg ggttcaggta tgccgggccg atcgatggac ataacttcga gcatctgttg
     2641 cccaccttgg aaaatgtatt gaagatgaag gggcctgtat tgctccatgt cgtgacgaaa
     2701 aaagggctgg gctatgagcc ctcggtgaag aatcctgtgt ggttccatgc ctgtcctccc
     2761 tttgtgcggg agaccggggt gccggcaaag aaagctgcgc ggccgtccta tacgcagatt
     2821 gccatcgaat cattagtcaa gctggcccga gcggacaagc gcatcgtcgc gattacagct
     2881 gcaatgtgtg aagggactgg cctgaatatt tttgagaaag agtttccgga tcggctctat
     2941 gacgtcggga ttgccgaaca gcatgcagtg acgtttgccg caggtcttgc gacccaaggc
     3001 ctccgtccgg tggtggcctt gtatgccacc ttcttacagc gtgcgtacga ccaggtggtg
     3061 cacgatgtcg cgacacaaaa cctgccagtg accttctgca tcgatcgcgg tggactggtt
     3121 gcggaagacg ggacgacaca ccacggggca ttcgattacg catttctgcg acacattcca
     3181 aacatggtcg tcatggcgcc gaaggatgaa aatgagctgc agcatatgat gaaaacctgc
     3241 gttctgcacg atgggcctgc ttcggtgcgc tacccgcgtg gactcagcct gggcgtcgcg
     3301 atggataagg agccggaggc attgccggtc ggaaagggtg agttgttgcg cgatggcacg
     3361 gaggtggcga ttgtggccat cggtgtgccg gtctcccatg ccgtgacagc ggcgaagcgg
     3421 cttgaagagg aaggtatttc cgcggcggtg attaatgccc gttttgtgaa gcctctggac
     3481 caggatttaa tcgtcgagct agcgaagcgg gttcgatatg tggtgacg
//
LOCUS       DATMXI010000006         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_86968, whole genome shotgun sequence.
ACCESSION   DATMXI010000006 DATMXI010000000
VERSION     DATMXI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86968"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..1476
                     /gene="galU"
                     /locus_tag="VN444_00095"
     CDS             571..1476
                     /gene="galU"
                     /locus_tag="VN444_00095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWR20243.1"
                     /translation="MRIRKAIVPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYVVEEA
                     AASGIEDIIIVTGRGKDAIEDHFDRSRELEIALRRQGKEEQLREIKRISELASFCYIR
                     QEEPLGLGHAILVARALVGNEPFAVLLGDDIIDAEVPCLKQMISAFERYQSSIIAVQQ
                     VSKEETSSYGIIDPKPVEDTIYQILDLVEKPSPAVAPSDLAIVGRYILTPEIFEALEQ
                     TPQDEGGEIQLTNGLRVLLRTQTMYGLAFHGRRYDAGSKLGFLKATLQFALKRPDLAP
                     GLREYLRTLNLGEVGAAVERDRKEN"
     gene            1482..>2631
                     /locus_tag="VN444_00100"
     CDS             1482..>2631
                     /locus_tag="VN444_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWR20244.1"
                     /translation="MADEKPVLSSIEGTVKSTEELSVPDAEIAETKTEGQARKVPETI
                     PLLPVRDVVIYPFMILPLFVGREKSVRAVDESLSRDRLILLVAQRDAEKEDPGADEIH
                     SVGTVAMIMRMLKMPDGRVKVLVQGLSRARVIGVERRELYFEARIAEVPETDLVTSDV
                     EVEALIRSVKELVSKGVALGKQISSDVVVIINNLEHSGRLADLVASHLDLKMEQAQEV
                     LELFDPTQRLKRVSELLSKELEVLEVQHRIQSQAREEMDKTHREYYLREQLKAIQKEL
                     GDTDDRSQELQELEKKIKKAKMPVAVESEAKAQLGRLSRMHPDAAEASVIRTYMDWLI
                     ELPWSRPTKDKLAIKQASKILNEDHYDLEKVKERILEYLAVRKLKKKMK"
BASE COUNT          624 a          651 c          825 g          531 t
ORIGIN      
        1 gagcatatga gcgcgttata ccagggtcgc tggctagcgc agcccgttat gaacaacaca
       61 gcgacaacga ccataacctt caatttcttc atgtgccccc ctttcaagct ctgaaagccg
      121 ctaatagcag caactgccct cttcgcatcg cggatggata acccacttat tcaaaaattt
      181 gtatatcgaa acaggatcct tgtcaacaca gaaaagatct gccgtgatgt tggacgcaga
      241 gctgctacgt tcagcccccg gtgcttgact ttttggcgaa agatgctata aaaaacaatc
      301 aaagtcattc aaaaggcgta tgaatcaaag gagctgagga tctttcgcga aagcgaaatc
      361 ctgtcgtgta gttttcggtc gcctgcgcct attcttactc gtctgggtgg ggaaggaata
      421 cgcttaagcg caaaaggccg ccgtccttag tgctggcttt tccagagcgg tcattagcag
      481 gcatgatccc tccttaggtt cgccagggat cttctcgtga ggttgcagcg ctctcttgca
      541 gttcgaacgg agtcagggtg ggcgtgatca atgcggattc gtaaagcaat tgtgcccgct
      601 gcgggtttgg ggacgcggtt cctccccgca acgaaggcac agccgaaaga gatgcttccg
      661 atcgtggaca agcctaccat ccagtatgtg gtggaggaag ctgctgcgtc ggggatcgag
      721 gatatcatca ttgtgaccgg gcgtgggaag gacgcgatcg aggaccactt tgaccgatcg
      781 cgggagttgg aaattgccct taggcggcag ggcaaggagg agcagttgcg ggagatcaag
      841 cggatctccg agttggcgtc gttttgctac atccgccaag aggagcccct tggcttggga
      901 cacgcgattc tggtggcaag ggcgctcgtg gggaatgagc cctttgccgt cttgcttgga
      961 gacgatatca tcgatgccga ggtcccctgt ctcaagcaga tgatctcggc atttgaacgg
     1021 tatcagtcct cgatcattgc tgtgcagcag gtcagcaagg aggaaactag cagctacggg
     1081 atcatcgacc ccaagccagt tgaggatacg atctatcaga tcctggatct ggtggagaag
     1141 ccgtctccag cagtagcccc gtctgaccta gccattgtcg ggcgctacat cctgacgcca
     1201 gagatctttg aggcccttga gcagaccccc caggacgaag gcggagagat tcagctcacg
     1261 aatggcctgc gagtgctgct gagaacacaa acgatgtacg gactcgcgtt ccacgggcgc
     1321 cggtatgatg ccgggagtaa gcttggcttt ttgaaggcga ccttgcagtt tgcgctcaag
     1381 cgtccggatc tggcgccagg gttgcgggaa tacctgagaa cactcaatct gggtgaggtg
     1441 ggagcagccg tggaacggga ccgaaaggag aactgagagc cgtggcggac gagaagcctg
     1501 tcctgagttc gatcgaagga acggtgaaaa gtacagagga actgtcggtt cctgatgctg
     1561 agatcgctga gaccaaaact gagggacaag ctcggaaggt acctgaaacg atcccgcttc
     1621 taccggtgcg agacgtggtg atctatccct tcatgatcct acctctcttt gtaggtcgag
     1681 agaaatcggt aagagcagta gacgaatcgc tttcgaggga ccgcctcatc ttgctggtgg
     1741 cgcaacggga tgctgagaag gaggatcccg gcgccgatga gatccactcg gtcgggaccg
     1801 tggcgatgat catgcgaatg ctcaagatgc cggacgggcg ggtaaaggtg ttggttcagg
     1861 gtctgtcccg cgccagggtc attggggttg agcggcggga actctacttc gaggcgagga
     1921 tcgccgaagt ccccgaaaca gatctggtca cgtcggacgt tgaggtggag gccctgatcc
     1981 gttcggtcaa ggagttggtg agcaagggcg tggccctcgg gaaacagatc tcgtcggatg
     2041 tggtggtgat tatcaacaac ctcgagcact caggccgtct ggctgacctc gtggccagtc
     2101 acctggatct gaaaatggaa caggcccagg aggtgctgga actgttcgat ccaacccagc
     2161 gcctgaagag ggtcagtgag ctgctcagca aggagctcga ggtgctcgag gttcagcatc
     2221 gaatccagag ccaggctcgg gaggagatgg ataagactca tcgggagtat tacctccggg
     2281 agcagttgaa ggccatccag aaagagctgg gtgataccga tgaccgaagc caggaactgc
     2341 aggagctgga gaagaagatt aagaaggcga agatgcccgt agcggtagag tctgaggcga
     2401 aggcgcagct agggcggctc agccgaatgc accccgacgc agcggaggcc tctgtcatcc
     2461 ggacctatat ggactggttg attgagctgc cctggagtcg gccgaccaag gacaaactgg
     2521 cgatcaagca ggcatcgaag atcctcaacg aggaccacta cgacctcgaa aaggtgaagg
     2581 agcgaatcct cgaatatctg gctgttcgca agctgaagaa gaagatgaag g
//
LOCUS       DATMXI010000007        19958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_64030, whole genome shotgun sequence.
ACCESSION   DATMXI010000007 DATMXI010000000
VERSION     DATMXI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 19958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19958
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64030"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VN444_00105"
     CDS             complement(<1..275)
                     /locus_tag="VN444_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20245.1"
                     /translation="MAPVQEALAVPDFRNPELAANRLHSIVDALRHHYQIAMVVEASS
                     YCDLACCFCAAHSALVSEKLSDGIGPVTKPEKHMKAATFASIINKLKG"
     gene            complement(278..1462)
                     /locus_tag="VN444_00110"
     CDS             complement(278..1462)
                     /locus_tag="VN444_00110"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWR20246.1"
                     /translation="MRKGIIHYIDKTELLDSYLDRLFSEIPPRDTWIRPLKEKVMLYG
                     AGGLGSLAVQMLVKAGIRPVAFIDMYSPLLACHGVPVRKPSEVGQDLREGCTVLVSVV
                     TTPYEPIYDYLSGLGFEDVRPFYDAAEYLIDELHLTNGWLATELTNTDILNIVDVYHE
                     LADNPSRAAYLRMLYWRIRREEVQSPLSNVTMTDKWFPAEVMPQFHANEYLVDAGAYD
                     GQVTAEFLKRVNCRFAGLLAFEPDPENMAGLEHYLSTLSPSVRDKIRLVPKGLGAEAG
                     YVPFLVGRNMASCISSKGAEMIPVVPLNDFVCEPVTVVKLHIEGAETDAIHGGMNLFR
                     LRRPIIAATTYHNADGMWRLPKAIMDGLEDYQFYLRVHGWCGISSILYAVPKERTVDP
                     GD"
     gene            complement(1486..2379)
                     /locus_tag="VN444_00115"
     CDS             complement(1486..2379)
                     /locus_tag="VN444_00115"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWR20247.1"
                     /translation="MKILVTGSTGFLGSHLMLHLRDRGHRVGCLVRDSSTLQPEPSNL
                     PTWRVGVNGSGFREALADFTPDVVVHLAALYVVEHRYENVGPLVQANVEFGTYLLDAM
                     GAAGCDALVYAGTSWQHYRNRDYCPVNLYAATKQAFSTLAEYYLDAADLRLLELHLYD
                     SYGEDDPRSKLVNRLKAAALSGNELAVSDGDQRLHLVHVADIVRGMAMACEQVCAFKA
                     GERRIYRLPSAKAVSLRELVAAWNAADPSRQVRVRWSSRPYRPREVFQPWEGAEILPG
                     WRADISLMEGLRRVRHGIGWG"
     gene            complement(2376..2978)
                     /gene="rfbC"
                     /locus_tag="VN444_00120"
     CDS             complement(2376..2978)
                     /gene="rfbC"
                     /locus_tag="VN444_00120"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWR20248.1"
                     /translation="MTPYRLKTRQHAMGSMFEMIDTVIPGCVEIRPRIYEDNRGRFVK
                     GWSRQVFADAGLYAEYAEEFYSVSRCGVVRGLHFQLPPMDQVKLVYCVSGRVQDAVLD
                     LRRNSFTYGRFALVELSAQVGNILYIPKGLAHGFCTLSETATMVYKVSSAYSREHDCG
                     VLWSSAGIPWSITDPILSDRDRAHPSFGAFVSPFDGEAAE"
     gene            complement(2975..4291)
                     /gene="rfbH"
                     /locus_tag="VN444_00125"
     CDS             complement(2975..4291)
                     /gene="rfbH"
                     /locus_tag="VN444_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HWR20249.1"
                     /translation="MNTVELRQNILDLVELYANQVFADQDFMPGITPIPPSGKVIGAR
                     ELQCVVDASLDGWLTSGRFNDAFERQLAAFLGVRHVLTTNSGSSANLLALSALTSPRL
                     EERAIRPGDEVITVAAGFPTTVNPILLYGAVPVFVDVHISTYNIDPEQIEAAVSEKTK
                     AIMLAHTLGNPFELAKVLRVARKYNLWLIEDCCDALGSTYDSKLVGTFGDIGTLSFYP
                     AHHITMGEGGAVVTNNGYLKPILESIRDWGRDCFCDPGKDNTCGKRFSWQWGDLPLGY
                     DHKYTYSHLGYNLKITDMQAAIGLAQLDRLPEFIEARRRNFRYLYERLTGLEEFLILP
                     RATPGADPSWFGFPISLREETGVDRLDMMRYLDQHRIGTRPLFAGNLTRQPYFQGRPY
                     RVVRELVNTDRITDRTFWVGVYPGLDLAHLDYLTGKLGRFFGVNVA"
     gene            complement(4248..5432)
                     /gene="rfbG"
                     /locus_tag="VN444_00130"
     CDS             complement(4248..5432)
                     /gene="rfbG"
                     /locus_tag="VN444_00130"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HWR20250.1"
                     /translation="MGKRQGAMESVVADRTFWQGKKVLITGHTGFKGSWLCLWLHLLG
                     AETTGFAMPPPTCPSLFELAHIDNLMTASIIGDVRDPEQITAAVRVARPEIVVHMAAQ
                     ALVRYSYANPVETYTTNVLGTVNILEAVRQVGSVRVVINVTSDKCYENRGWLWGYRED
                     ESVGGYDPYSSSKGCSELISAAYRNAFFKSAEYGRHGVALATVRAGNVIGGGDWAEDR
                     LIPDTLRSFLERRPASIRNPEAVRPWQHVLEPLGGYLMLAKYLWEDGAAFAEAWNFGP
                     DDDDVRAVEWVVDRLAGLWGAGASWRLDGDPHQPHEARYLKLDCAKAKARLGWHPRWG
                     LEQALQKIVTWYRAYHDHADMRTVTLAQIAAYLASSGNMVSNTKNPHEYRRTETEHTR
                     FS"
     gene            complement(5396..6166)
                     /gene="rfbF"
                     /locus_tag="VN444_00135"
     CDS             complement(5396..6166)
                     /gene="rfbF"
                     /locus_tag="VN444_00135"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HWR20251.1"
                     /translation="MKAVILAGGGGTRLSEETVGRPKPMVEIGGKPILWHIMKLYSAY
                     GVNDFVVCLGYKGYMIKEYFANYFLHRSDVTIDLAHNRVEVHQNHVEPWRVTLVDTGE
                     QTMTGGRLKRVADYVQDEAAFYFTYGDGVADIDLSAEMAFHRTHGRLATVAAVQPPGR
                     FGALDLDQERVCGFTEKPRGDGGRINGGFFILSPEVTKLIENDATIWEGDLLARLSAM
                     GELMAFEHRGFWQAMDTLRDKYCLEELWASGKAPWKAW"
     gene            complement(6839..7618)
                     /locus_tag="VN444_00140"
     CDS             complement(6839..7618)
                     /locus_tag="VN444_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWR20252.1"
                     /translation="MVQLAKLRTVLALTVVLSTISACAVAPIDHLDYQQQLMLKVGQG
                     DQPLTLEQVKVILATTRNFTGMNLRFLKLTSLDFSGAVLDKANLYGADLRGVSLRHAS
                     LQETDLRETDLRSADLNKASLVRADLKAANLSKANLEKADLREAKMQRAMIRSANLRN
                     ADLLKADLDEAILQLADLSDARLYGATLRGTDLSRATLRGANLSLSDLRGAKLQKASS
                     HDADFREAHLDEADFTEAHVRDTFNLEAAANGDAVKGLSFR"
     gene            8100..8399
                     /locus_tag="VN444_00145"
     CDS             8100..8399
                     /locus_tag="VN444_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20253.1"
                     /translation="MTDNKVAEQLQSVCDIVSRQTLPHRLCGPVPQLTYCTSPDTPLS
                     VTGGVYDEHAGRSDSPFQPSGLRRAPCGWWVFHPEERVELVDGEIIMMALQSSRQ"
     gene            complement(8449..8718)
                     /gene="csrA"
                     /locus_tag="VN444_00150"
     CDS             complement(8449..8718)
                     /gene="csrA"
                     /locus_tag="VN444_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HWR20254.1"
                     /translation="MLILTRKLGEKITIGESIVISVIEVRGSQVRLGISAPPEVTIHR
                     GEIYERIREQNLQASALGLSHTGALSERLRVIFQESASSKKPSTE"
     gene            complement(8725..9129)
                     /gene="fliW"
                     /locus_tag="VN444_00155"
     CDS             complement(8725..9129)
                     /gene="fliW"
                     /locus_tag="VN444_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HWR20255.1"
                     /translation="MDVASAEQTVFYFPQGLPGFEELARFFLCDREGLQPLTLLIALD
                     TSDVAIPLLRCADFLTDYSPPIPATDLEALEARGMEELELFVVVSFEGNGGVAANLMA
                     PICVNRARRLGRQVVLPDGTYPLQYSLLPERE"
     gene            complement(9178..10098)
                     /locus_tag="VN444_00160"
     CDS             complement(9178..10098)
                     /locus_tag="VN444_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR20256.1"
                     /translation="MRITSNTIFHQLTADINRAANRLFERQRQVASSRRMATASDDPV
                     GAGLAVSLRESLAQLAQAQRNGDQAEARLQASADILTGILSDLRDVKELAFRGMDGAF
                     TLSDRQNLATQVNQKLERLLSDANARSIDGHLFGGTQTTVAPFTATRDVNGDITAVTA
                     NPLGIAGQVNTEIPGGLRMTVNVPGSTVFTATSPQTVDIFPLLVTVRDQLRAGNVAGV
                     EANLADLDAAVDQVRVVGADVGSRIGRIRDIQARSQNDLLTLRGRLSRIEDTDVAEAS
                     IEFQQAQNVYQAALAAAARVGQINLLDFLR"
     gene            complement(10136..10537)
                     /locus_tag="VN444_00165"
     CDS             complement(10136..10537)
                     /locus_tag="VN444_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWR20257.1"
                     /translation="MSMLIERLKREHGSLVDALNKVKELGVGSKEGQNKLLSAKIGLL
                     AHLKAEDGQLYPALNQEAKNNDSLRRTLDLFARDMEMVSKDALNFFNKYAHGGSGLEF
                     AKDFGRLYTVLSQRITKEEKSLYPEYDKLSR"
     gene            complement(10619..12043)
                     /gene="flgK"
                     /locus_tag="VN444_00170"
     CDS             complement(10619..12043)
                     /gene="flgK"
                     /locus_tag="VN444_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWR20258.1"
                     /translation="MISLQASLTMARKALQAQQVAIQTTGHNIANANTPGFTRQRVDL
                     TPAVPFSLGSVGSLGTGVDIKDITRIRDLLLDSQFRDAHQALSRQEAEEATLSQIELI
                     VGEPSKNGLAAGLSALFDSFQNLANHPTNLAVRSVVRDKARALADQFHRLDDGFERLK
                     IDLHNEIQVVVKQVNGLAQQIADLNRRIAMSEGGAGSANDLRDQRDQALDELSKLVGG
                     SLIEDANGQVRVTVGGGLTLVDGVTAVPLEAEEFDQTDPTYPDSVRLFLGGNVLTPGG
                     GQLAGLLNSRNSADGFVKGFQTQLDTLAKAVVDEMNAVHTTGYGLDGNNGYDLFTPLT
                     AAAGAARFISVDAAIEADVMRIAASANGDPGDNNMAVALAGLQDDSTVITLPSLQSVT
                     FGSYLSNLVSDLAEQEAGAKRSVDLHTTMADFLTGRRDQSSGVSLDEEMTDLIRFQKA
                     YEAAAHFANVVNDLLGTLMSQLGR"
     gene            complement(12056..12556)
                     /locus_tag="VN444_00175"
     CDS             complement(12056..12556)
                     /locus_tag="VN444_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWR20259.1"
                     /translation="MMMLLEDLAQVLEQETEKYEALLRLLRQERGLIVKGDLGALAEL
                     MKRKETLALELKIIQEARGALMTRVSATYDIPLAELALFRLADLVPASHTMSYRRLLN
                     RLAVLVTRLVEENDSNAALLDRTVAYIKGSLSFLTSAVTPVPLYQGDGSIVSPAPSLS
                     VLNSQA"
     gene            complement(12665..12973)
                     /gene="flgM"
                     /locus_tag="VN444_00180"
     CDS             complement(12665..12973)
                     /gene="flgM"
                     /locus_tag="VN444_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWR20260.1"
                     /translation="MKIENRGDSANINPCLNRVQDAAERPESKQSAPAHDAPADRVEL
                     SQAAKALQQARTLLAASPEVRPEKVAELKGMIQRGVYNVKGREVAAKMIGQGLFDHLV
                     "
     gene            complement(13042..13392)
                     /locus_tag="VN444_00185"
     CDS             complement(13042..13392)
                     /locus_tag="VN444_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HWR20261.1"
                     /translation="MDPGKVSQGLHAVQQRTRPEEQKLREVAQGFEAILIASLLKEAR
                     PSEQAGLFGGGLSQDLYRQLFTDEIAKAIARSGGIGVGKMLEQQLRATLRAGVYENRL
                     KEPSQSTDITKRDR"
     gene            complement(13411..14490)
                     /locus_tag="VN444_00190"
     CDS             complement(13411..14490)
                     /locus_tag="VN444_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWR20262.1"
                     /translation="MGAVTALLPAPAMAEARIKDIAKIQGVRENELFGYGLVVGLNGT
                     GDRAQVTFTIQAVVSMLKKLGITVPAERLTLKNVAAVMVTAKLPPFAKAGSTLDVTVS
                     SMGDATNLQGGMLLITPLQAADGQVYAVAQGPISIGGFNFEAGGTGEKVQKNHPTVGR
                     VPSGAIVEREPSADFLKAQQLLLVLTNPDFTTAIRMAQAVGHSLGAETRAKDASTVEI
                     RVPDSYLSNPVELIAAIENLSLVPDMTAKVVVNERTGTIIMGSQVRISTVAVAHSNLS
                     LQIRSELLVSQPAPLSEGKTTVVPRTEIEAREDRTRVNLMSEGVSIGDVIKALNALAV
                     TPRDMISLLQAIKAAGALQGELQIM"
     gene            complement(14680..15270)
                     /locus_tag="VN444_00195"
     CDS             complement(14680..15270)
                     /locus_tag="VN444_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWR20263.1"
                     /translation="MKRWCAGLAAVLWLCGGTASGESLWRDIGSGFLFVDTKAQHVGD
                     IVTVLVTESSSLNRQAETNTKKESTNSGNLSSLFGLPLGDKTQYAFEGANEHKGSGSI
                     TRSDEVTARVVARVVKVLPSGNLIIEGRRAVRANDESQYVAISGVIRQVDITPANTIL
                     STQIADAEIVLEGKGAIAEKQRPGLLNRFADWLFLY"
     gene            complement(15350..16471)
                     /gene="flgA"
                     /locus_tag="VN444_00200"
     CDS             complement(15350..16471)
                     /gene="flgA"
                     /locus_tag="VN444_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWR20264.1"
                     /translation="MTRRRRWQAKHGQRSLPAPIRTLQGCTVKLSGVLFRLPRAIQVA
                     GVVLAIVIAVVRSALAGQGLPLEQRERVPRAVIRVKESATVRGKEILLKDLAEVTAQS
                     QSLSEALEALPVGQAPPPGLTRMFDPDLIVIKLRQYKIDPSGIRIESPRHVVVAGAHR
                     VIESDELFQAAKSAVLKGREQELERITVRPDTLPSALVVPPGEIELRARPRFQSSVGL
                     GSIPVIVEVWIDGRLYRAVSLAVRLSLIREVVVVNYPLPRHTVVKGTDVRLERRDIGP
                     LAHEPLQDLTLAVGRRTTRMLAMGEVVVSESVELPPLIQKGEVVTLMVETPRLLVTAK
                     GIAQEGGKAGQLVRVKNTASGREVLGKLESDKTIRIGSE"
     gene            complement(16532..17320)
                     /gene="flgG"
                     /locus_tag="VN444_00205"
     CDS             complement(16532..17320)
                     /gene="flgG"
                     /locus_tag="VN444_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HWR20265.1"
                     /translation="MIRALFSAVAGMQAQQLNLDNIANNLANVNTTGFKRSRLDFQDL
                     FYQTYRPAGASTASGAEIPVGIQVGHGSKPIASQRIYIQGDPQQTENPLDLMIEGDGF
                     FQVLRPDGTIAYTRAGSFKRDANGRVVTSDGFALQPEVSIPAQAMTIHVGSDGVVSAT
                     IPGQTASQQIGTIELSRFINPAGLQSVGRNLLLPTSASGDPTSGAPGTQGLGTIGQGF
                     LELSNVKVVEEMVNLIMSQRAYEAGSKAVQAADEMLQVSNNIRR"
     gene            complement(17338..18075)
                     /locus_tag="VN444_00210"
     CDS             complement(17338..18075)
                     /locus_tag="VN444_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HWR20266.1"
                     /translation="MVSGLYTAASGLMAQMAKMEALSQNLANAGTPGFKGDLLVIEGG
                     ARGPLQSALLIEGRPEGPQPVRIGRRVTDFGRGLIRETQNPLDLANSGPGYFVVQTPQ
                     GVRYTRAGNFAIDADGHLETLDGSRVQGKKGAVQIPGGRFIVDNGGRILVEGQEVDRL
                     RIVDPDSTTLIRGGGSLFTLKPSAPEPPVAKAVDIRQGFLEGSNVDAVTAMTQIMVSL
                     RASEAYQKAIHALDQSLNQAASELGRI"
     gene            complement(18192..19199)
                     /locus_tag="VN444_00215"
     CDS             complement(18192..19199)
                     /locus_tag="VN444_00215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWR20267.1"
                     /translation="MTDHSAKILFIRVDPSLPHEAVERLMCEGFEVKTPSNPCQALYE
                     MYADPPDLMILSDDLSASEGERIARLIRADPVFRQLPMILLLQTGADERVDTWAELQL
                     SDFVAAPVSPQEVCIRSRLCLLRSNLELDANPLTRLPGNNTILQEIQRRIDADQAFAL
                     AYLDIDSFKAYNDRYGFARGDEALRMTARIILNAVMNLGSGSGFVGHIGGDDFVVILP
                     MEQIESACREIVANFDLIAPSLYDETDRRQGSLLLKDRRNRLCRFPLMTLSIAVVPSE
                     GRGFEHVGQLVSRAAELKTAAKAKPGSVYLVDRRLVVDCAYADGSGTAANFSPSPVVL
                     S"
     gene            complement(19212..>19958)
                     /locus_tag="VN444_00220"
     CDS             complement(19212..>19958)
                     /locus_tag="VN444_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWR20268.1"
                     /translation="SPETSVADVGRVIAKDQVLSARVLKLVNSAFYGFSRRVSTVTHA
                     TVLLGFNVVKGLVMSASVFDLFQQGRGDGLDRKHLWDHAIGTARIAGVLARKAGLHEP
                     EEAVVAGLLHDLGKIVLCADLPEEFTATVGLAKKRNLLMIEAEMEVLGTTHAEVGRWL
                     LEHWKLPVKLVEPIALHHVPHLAREATLTTAIVHLADILCIAASIGDSGDAKVPPLSH
                     TAWNLLDLTMGQVDEALSEVDEQEITATFA"
BASE COUNT         4333 a         6124 c         5291 g         4210 t
ORIGIN      
        1 cctttgagct tattgattat ggatgcgaac gtcgcggcct tcatatgctt ttcaggtttg
       61 gttaccggcc cgatcccgtc agagagtttt tccgaaacca aagcgctgtg cgcggcgcag
      121 aagcaacagg ccaaatcgca ataagaggaa gcctcgacca ccatggcgat ctgataatga
      181 tgacgcagcg catcaacgat actgtgcagt cgattggcgg ccagttctgg attacgaaaa
      241 tctggtacgg caagcgcttc ttggactgga gccatactta gtcacctgga tctacggtgc
      301 gttcctttgg aacagcgtag agaattgatg aaatgccgca ccagccgtgc acgcgcagat
      361 aaaactggta atcttccaga ccatccatga tagccttggg caaccgccac atcccatccg
      421 cattatgata tgtggtagcc gctatgatcg gccgccgcaa tctgaacaga ttcataccgc
      481 catgaatggc gtctgtctcc gcaccctcaa tatgaagctt caccaccgtg acgggctcgc
      541 aaacaaaatc attcaacggg acgacgggga tcatttctgc gcccttggaa gagatacacg
      601 aagccatatt cctgccgacc aggaatggta cgtacccagc ctcagctccc aggccctttg
      661 ggactaaccg aattttatcg cgtactgaag gcgataaggt tgaaaggtaa tgttccaatc
      721 ctgccatatt ttccggatcc ggctcaaaag ccaacaggcc agcaaatcgg cagttcaccc
      781 gtttgagaaa ctcagccgtc acctgaccgt cgtaagcgcc ggcatcaaca agatattcat
      841 tggcatggaa ctgcggcatg acttcggcag gaaaccactt gtcggtcatg gtcacgttgc
      901 tcaacggcga ctggacttcc tcacgacgta tgcgccagta gagcattcgc aggtaggcgg
      961 cgcgtgacgg gttatcggcc agttcgtgat agacgtcgac aatattgagg atgtcggtgt
     1021 tggttaattc cgtggcaagc cacccgttcg taaggtgcaa ctcgtcaatg agatattcgg
     1081 ctgcatcata aaatgggcgc acgtcctcaa agcccaagcc ggacaagtag tcataaatgg
     1141 gttcataagg agtggtaacg acgctgacta aaacagtaca accttcccgc aggtcttgcc
     1201 cgacctcgga cggcttcctc accggtaccc catgacatgc aagtagaggc gaatacatat
     1261 caatgaaggc tactgggcgt attcctgcct taaccaacat ctgtacggcc aggctgccca
     1321 accctcccgc accatacagc atcacctttt ccttgagcgg cctgatccag gtgtcccttg
     1381 gtgggatttc agagaacagg cgatccaggt acgaatcgag caactccgtc ttatcgatat
     1441 agtgaatgat gccttttcgc attaccgatg gcccttgatc catcgctatc cccacccaat
     1501 tccgtgccga accctacgta aaccctccat caatgaaatg tcggcgcgcc agccaggcaa
     1561 tatctcggct ccctcccacg gctggaacac ttcacgcggt cggtaaggtc gcgaactcca
     1621 ccgcaccctg acctggcggg acggatcggc ggcgttccaa gcggccacca gctcacgcag
     1681 ggaaaccgcc ttggccgatg gtagccgata gatacggcgt tcccctgcct tgaacgcgca
     1741 gacctgctcg caggccatcg ccataccccg gactatgtcc gccacatgga cgagatgaag
     1801 ccgctggtcg ccatctgaca cggctaattc attgcctgac agggccgcag ccttgagcct
     1861 gttgaccagc ttactccttg ggtcatcctc accgtagctg tcgtagaggt ggagttccag
     1921 gaggcgcaga tccgctgcgt ccaagtagta ctccgcgagg gtcgagaagg cctgtttggt
     1981 cgcggcgtag agattgaccg gacaatagtc ccgattacgg tagtgctgcc aggaagtacc
     2041 cgcatagacc agagcatcac aaccggctgc ccccatggcg tccaacagat atgtgccgaa
     2101 ttccacgttg gcctgcacta agggtcccac attctcatat cgatgctcga ccacatacag
     2161 ggcggccaga tgcacgacaa catcgggggt aaagtcggcc aacgcctcgc gaaagccgga
     2221 gccgtttact ccaactctcc aggtcggaag attggacggc tccggctgca gggtcgagct
     2281 gtcacgcacg agacagccca cacgatgccc gcgatcgcgc aggtgcagca tcagatggga
     2341 accaagaaag ccggtcgagc cggttacaag aatcttcatt cggcagcctc accatcgaaa
     2401 ggactgacaa aggctccaaa ggaagggtgc gcccgatctc gatcggatag gatcggatcg
     2461 gtaattgacc acggaatacc ggcggaactc cacaatacgc cgcagtcatg ctcccgtgaa
     2521 tatgcgctcg acaccttgta gaccatggta gccgtctcac tcagtgtaca gaaaccatga
     2581 gccaagcctt tcggaatgta gagtatattt ccgacttggg cggacagttc gaccaaggca
     2641 aagcggccat acgtgaagga gttccgacgc agatccaaca ccgcatcctg aactcgtccg
     2701 ctcacgcagt aaaccagctt cacctgatcc atcggcggta actggaaatg caaccctcga
     2761 accaccccac aacgagaaac agaatagaat tcctcagcat actctgcgta aagacccgca
     2821 tcggcaaaga cctgtcggct ccaccccttt acgaaccgac cgcgattgtc ctcgtaaata
     2881 cggggccgta tttctacgca acctggaata accgtatcta tcatttcgaa catgctgccc
     2941 atggcgtgct gtctcgtctt cagtcggtac ggcgtcatgc gacatttacc ccaaagaacc
     3001 gtcccagctt ccctgtaaga taatccagat gggccagatc cagtcccgga tatacaccga
     3061 cccagaaggt ccggtcggtg atcctgtcgg tattgaccaa ttcgcggaca actcgatatg
     3121 gacgcccctg gaaatacggt tgccgggtta ggttacctgc aaacagcggt ctggtaccga
     3181 ttctatgctg atcgagatat ctcatcatat cgagtcggtc cacaccggtc tcttcgcgca
     3241 ggctgatcgg aaaaccaaac caggagggat cggctccggg cgttgcccgc ggcaggatca
     3301 ggaactcttc gaggccggtc agtcgctcgt agagataacg gaagttacgc ctccttgcct
     3361 cgatgaactc aggcaaccgg tccaactggg ccaaaccaat cgccgcctgc atatcggtaa
     3421 tcttgaggtt atagccgagg tgggaatagg tgtacttatg gtcatacccg agaggtaagt
     3481 caccccactg ccaactgaaa cgcttgccgc aggtattgtc cttaccgggg tcgcagaagc
     3541 aatctcggcc ccagtcgcga attgattcaa ggataggttt taagtatccg ttgttggtaa
     3601 ctactgcgcc accctcgccc atcgtaatat ggtgcgcagg atagaagctg agcgtaccga
     3661 tgtcgccaaa ggtgcccacc agcttactat catatgttga gccaagggca tcacagcagt
     3721 cctcgatcag ccaaaggtta tatttcctgg ctacacgcag taccttggcc agctcaaagg
     3781 gattccctag ggtatgggca agcatgatgg ccttggtttt ctccgaaacc gccgcctcaa
     3841 tctgctcagg gtcgatattg taggtggaga tgtgcacgtc cacaaacaca ggcaccgcgc
     3901 cgtacaacaa gatgggattg accgtagtgg ggaagccggc tgccacagtg atcacctcgt
     3961 cgccaggcct gatggcccgc tcttcaagtc gcggagacgt gagcgcggac aatgcgagca
     4021 gattggcgga cgatccggaa ttcgtcgtca gcacgtgcct gactccgaga aatgcggcaa
     4081 gctgcctttc gaacgcatcg ttgaaacggc cgctagtcag ccaaccgtcc agagaggcgt
     4141 caaccacgca ctgcaattcc cttgccccga tgaccttgcc ggatggcggt atcggcgtaa
     4201 taccgggcat aaagtcttga tccgcaaaga cctgattcgc gtataactca actaaatcga
     4261 gtatgttctg tctcagttcg acggtattca tgtgggttct tggtgttaga aaccatattc
     4321 ccggaagatg cgagatatgc agcaatctgg gccaacgtca ccgtacgcat atctgcgtga
     4381 tcatggtagg cccggtacca cgttacgatc ttctgcagcg cctgctccag cccccaacgg
     4441 gggtgccaac ccagccttgc cttggccttg gcgcagtcca acttaaggta ccgcgcttca
     4501 tgcggctgat gcgggtcacc gtcgagtcgc caggatgcac ctgcgcccca taagccggct
     4561 agacggtcta cgacccactc gacagccctg acatcgtcgt catctggtcc gaagttccac
     4621 gcttcagcaa acgccgctcc atcctcccag aggtactttg caagcatcag ataaccgcct
     4681 aacggttcaa gtacgtgctg ccatggtcga accgcctcag gattgcgaat gctcgccgga
     4741 cgtctctcca ggaaagatcg cagggtatcc ggaataagcc gatcctctgc ccagtcgccg
     4801 ccgccgatca cattacccgc ccgtactgtg gccaacgcca cgccatggcg accatactcg
     4861 gctgatttga aaaacgcgtt tcggtaggcg gcggagatga gctccgaaca gcccttgctg
     4921 ctgctgtacg gatcgtagcc gcccacggat tcatcttccc gataacccca taaccatcca
     4981 cggttttcgt agcacttatc gctggtaaca ttgattacaa cgcgtacgct gcccacctgt
     5041 cggaccgcct ccagtatgtt gactgtaccc aagacattgg ttgtgtaggt ttctacagga
     5101 tttgcatagg aataccgcac caacgcctga gcggccatgt ggacgacaat ctctggcctc
     5161 gctactcgca ccgcagcggt aatctgttcc ggatcgcgca catcgcctat gatggaagcc
     5221 gtcatgagat tgtcgatgtg ggccaactcg aacaaggagg ggcatgtagg tggcggcatc
     5281 gcaaaacccg tcgtctcggc tcccagcaga tgtagccata gacagagcca gcttcccttg
     5341 aaaccggtat ggccggtaat caagaccttc tttccctgcc agaacgtgcg atccgctacc
     5401 acgctttcca tggcgccttg ccgcttgccc ataattcttc gagacagtat ttgtcccgca
     5461 gagtatccat tgcctgccag aaaccgcgat gctcaaaggc catcagttcc cccatagccg
     5521 acagccgagc cagcaggtct ccttcccata tcgtggcgtc attctctatc agcttcgtaa
     5581 cctccggcga gaggataaag aacccgccat tgatccgtcc tccatcgcca cggggctttt
     5641 ctgtaaagcc acacactcgt tcttgatcca gatcgagcgc tccaaagcgt ccaggcggct
     5701 ggaccgccgc aacagtcgcg agcctgccat gtgtccgatg aaacgccatc tcggcggaaa
     5761 ggtcgatatc agcaacccca tcaccgtatg taaagtaaaa cgccgcttca tcttgcacat
     5821 aatcggctac acgcttcagg cgcccgccgg tcatcgtctg ttcgcccgtg tccaccaggg
     5881 tcacgcgcca gggttcgaca tggttttgat gcacttccac acgattgtgg gctaaatcga
     5941 tcgtgacatc ggagcggtgc agaaaataat tagcgaaata ctctttaatc atatagcctt
     6001 tatacccaag acagacgacg aagtcattca caccgtaagc cgaatagagc ttcatgatat
     6061 gccacagaat cggcttcccc ccaatctcta ccatcggctt ggggcgaccg accgtctcct
     6121 cactcagccg cgtacctcct cctccagcca gaatgaccgc tttcataatc aacgctcact
     6181 tcgataaaag gtcagatcgt tttggtttcc actcgcattt cagcattgtc atgaatgaca
     6241 agaggttatc ggagttggga tgaggtcaat ctatgctgtt cggttgcgag agcatgagag
     6301 cgtcacgggt tacatttcac acccgccatc aattgctcac cgatctcgtc gaagatttac
     6361 gcaaatcatg tgccaggaga aaaaggggtg ggattagcgt tgaacgcgct aaaaaggtgc
     6421 agttcgagag aggtatgacg accttcgtga cgtgaagagg tcaccgaacc cgtcacgcgg
     6481 gcggaaaatt tttccgtccg acgaccaagg atcaatcgtt cacagtgaga ttactggagg
     6541 gaaggtctgg cctcgtcact cacatcggaa tcgaggattc gattccgatg tgctgcgggc
     6601 agtcgaatcg aatgaccaat gatgtcggta atgagcagtt cagggacacg cgacgtcgag
     6661 aacggcaagt gcacggggaa aaaagggctc gctctcaaaa acagatttgg actcgtatag
     6721 gtaaccttcg tctcgatctg gggttgagac tgcgatgaga atcgctacgg acccaaggag
     6781 ctctgtatca ccaatactgc gctgcaatgg accatcaacc ccctcgcttc tgccacactc
     6841 agcgaaaact cagacctttc acggcatcac cattcgccgc cgcctcgaga ttgaaggtat
     6901 ctcgcacatg ggcctcggtg aaatcggctt catcgagatg ggcttcacgg aaatcggcat
     6961 cgtgagaaga ggccttctga agctttgcgc cgcgtagatc gctcaggctg agattggccc
     7021 ctcgtagggt tgcccggctg agatcagtgc cccttagggt agctccatag agccgagcat
     7081 cgctgagatc ggccaactga aggatggctt cgtccagatc agctttcaat agatcggcat
     7141 ttctgagatt cgcagatctg atcatggctc gctgcatctt tgcttcgcgc agatcggctt
     7201 tttcaagatt tgccttgctc agattcgctg ccttgagatc ggctcgaacc agagacgcct
     7261 tattgagatc ggccgaacga agatcggttt cacgaagatc ggtctcctgt agtgatgcat
     7321 gccgcaggct gacacctcga agatcagcgc cgtatagatt ggccttgtca aggacagccc
     7381 cgctgaaatc gagcgaggta agcttcagaa atctcagatt cattcccgta aaatttcttg
     7441 tcgtggccag aatgaccttg acttgctcca gggtcaatgg ctgatcgccc tgtccgacct
     7501 tcagcattaa ctgttgctga taatcaaggt gatcaatggg cgcgaccgca catgccgaaa
     7561 tggtcgacag caccacggtc agagcaagaa ccgtccttag cttcgctagt tgcaccatgt
     7621 cgctctccta tgatggctac tcacttaaca catagtcgga tggacacgca cgtactgacg
     7681 cggtccgcat tgacctcgca cacgtcgtta ttgaggcctc atcaacaacg gtcagtctat
     7741 gccgatgatc gagaggcata accacacatg ccgaacggct caacggcact gcgacaagcg
     7801 ctagaactgt ccagagtttc attgtgatca tctcctcatg aatattccat tggttatcac
     7861 cttatccgat gggacgatct attaatcagg tgcgagacga cgaagtggtt gagctgccct
     7921 atcttggcac atatctcaga atcacctcgc cttaatccat tatcggctca tcgagaagct
     7981 cgcttgagca aaaacacaga ctcattcttg acagtgtctt ttcaagaggt cctgcccaac
     8041 agctttggtg taatccgtga gacgacgtac tcggcgtatg gaagggaata ggtcggggca
     8101 tgactgacaa caaggtcgca gagcaactcc agtcggtctg cgatattgtt tcccgccaga
     8161 cactcccaca tcgcctctgt ggtcccgttc ctcaactgac ctactgtacc agccctgata
     8221 caccgctttc agtgacagga ggagtctatg atgagcatgc aggccgttcg gactcgccat
     8281 ttcagccgtc aggactacga cgagcgcctt gtggctggtg ggtttttcat ccggaggaac
     8341 gggtggaact ggtggatggc gagattatca tgatggcact acaaagcagc cggcagtaag
     8401 gaccgttcgc ttggcagaag acatgctctg tgcggacctg caagaacatt actcagttga
     8461 gggcttcttg gatgaggctg actcttggaa gatgacgcga agccgttcgc tcagcgcccc
     8521 tgtgtgtgag aggccgagcg cgctggcctg aaggttctgt tctcgaatcc gctcgtagat
     8581 ctcgccccga tggattgtca cctctggtgg cgccgaaatg ccgaggcgga cctgcgaacc
     8641 gcggacctct atgacgctaa tgacgatcga ttcaccgatc gtaatcttct cgccgagctt
     8701 cctcgtcagg atcaacatcg cggctcattc gcgttccggc agcagcgaat actgtaaagg
     8761 gtaggtcccg tctggaagca ccacctgtcg gccaagtcga cgcgcacggt tcacacagat
     8821 cggggccatg aggttagctg ccacgccgcc gtttccctca aacgaaacga ctacgaacag
     8881 ctccagctcc tccatacccc tcgcttcaag ggcttccagg tccgtcgccg gaatgggtgg
     8941 cgaatagtcg gtcaggaaat cggcgcacct gagcaatggg atggcgacat ccgaagtgtc
     9001 cagtgcgatc aggagcgtca gcggctggag cccttctctg tcacacagaa agaacctggc
     9061 cagctcttcg aagcccggca gtccttgggg gaagtagaac acagtctgtt cggctgacgc
     9121 gacgtccatg acaacggatc cctgagtacc gaccaactcg cgcttcatcg tcatacgcta
     9181 acgcaggaaa tcgagcaggt tgatctggcc gaccctggcg gctgcagcca gagcggcttg
     9241 gtagacattc tgcgcttgct ggaattcgat agaggcctca gccacatcgg tgtcctcgat
     9301 cctcgacaga cggccccgca acgtcagcag atcgttctgt gaccgcgcct gaatgtcgcg
     9361 aatacggccg atcctggagc cgacatcggc gccgaccacg cgcacctggt cgacggcggc
     9421 atcgagatcg gccagattcg cttcaacccc cgcgacattt cccgccctca actggtcccg
     9481 aactgtaacc aggagcggga agatatctac cgtctggggg gaggtcgcgg taaagactgt
     9541 cgagcctggc acattgaccg tcatcctcaa cccgccaggg atctccgtat tgacctgacc
     9601 ggcaatccca aggggatttg ccgtgacggc agtgatatcg ccattcacgt cgcgcgtggc
     9661 ggtaaatggc gccacagtgg tctgtgtgcc gccaaagagg tgcccatcta tcgagcgcgc
     9721 gttggcgtcg gacaacagcc gctccagctt ctgatttacc tgggtcgcca gattctgccg
     9781 gtccgacaga gtgaaggcgc catccatacc tcgaaaggcc agctccttca cgtctctcag
     9841 atctgaaagg atgcccgtca atatgtctgc tgacgcctgt aatctggcct cggcctggtc
     9901 accgtttcgc tgggcctgcg ccagttgggc cagcgactcc cgaagcgaga cggccaagcc
     9961 ggcgcccacc gggtcgtctg aggcggtcgc catccgtctt gagctggcaa cctgacgctg
    10021 ccgctcaaac agcctattcg cggccctgtt gatatcggct gtcaattggt ggaagatcgt
    10081 attgctcgtg atgcgcatcg gcgtcccccc atccaacgtg ttgagctttg cgtcatcatc
    10141 gactcagctt gtcgtactcc gggtacaggc tcttttcttc cttggttatt ctctggctga
    10201 gaaccgtata cagccgaccg aagtctttgg caaattccaa ccccgaacct ccatgcgcat
    10261 atttgttaaa aaagtttaag gcatctttag ataccatctc catgtcgcgg gcaaacagat
    10321 ccagggtccg cctcaacgaa tcattgtttt tcgcttcttg atttaaggcc ggatacaact
    10381 gcccatcttc cgccttgaga tgcgcaagca agccgatttt cgccgagagc aatttattct
    10441 gtccttcctt tgagccgaca ccaagctcct tgactttgtt aagagcgtca accagcgacc
    10501 catgctctct tttcaatctt tcgattaaca tggacatggc atctctcctt tgtgcgtcat
    10561 aatacctgtg accggcgtaa ccgtccacgc gtctaacgtg agatcacgcg acttcgatct
    10621 agcggccaag ttggctcatc aacgttccca gaaggtcgtt caccacattt gcaaagtggg
    10681 ccgccgcctc gtacgccttc tggaaacgga tcaggtcggt catctcctcg tccagcgaga
    10741 caccggaaga ctgatctcgt cggccggtca agaagtccgc catcgttgtg tgcaagtcga
    10801 ccgaccgctt cgcacccgct tcctgttcgg cgaggtcgct gacgaggtta ctcaggtaac
    10861 tgccgaaggt gacgctctgc aacgacggca gcgtaatcac cgtagagtca tcctgaaggc
    10921 ccgccaaggc gaccgccata ttgttgtccc ccggatcacc attcgccgat gccgcgatcc
    10981 tcatcacatc cgcctcgata gccgcatcga ccgagataaa ccgggcggct cccgccgcag
    11041 ccgtcaatgg cgtaaacagg tcgtaaccat tgttcccgtc caggccatag ccggtcgtgt
    11101 ggacggcatt catctcgtca actacggcct tcgccagggt atctagctgg gtctgaaacc
    11161 ccttcacgaa gccgtccgcg gagtttctgg agttgagcag tccggccaac tgtccgccgc
    11221 cgggtgtcag tacgttgccg ccgaggaaca ggcggacgct gtcgggatag gtcggatcgg
    11281 tctgatcgaa ttcctccgct tccagcggga cagccgtcac tccatccacc agtgtcagcc
    11341 ccccaccaac cgtcacacgg acctgaccgt ttgcatcctc gatcaacgac ccacccacga
    11401 gcttcgacag ttcgtcaagt gcctggtcgc gttggtctcg cagatcattg gccgatccgg
    11461 ctccaccttc agacatcgca atccggcggt tcaggtcggc aatctgctga gccagcccgt
    11521 tgacctgctt cacgactacc tgaatctcgt tatgcaggtc gatcttcaac cgctcgaacc
    11581 catcgtccag gcgatgaaac tggtccgcca gcgccctggc cttgtcgcgg actacggacc
    11641 tgaccgcgag attggttgga tggttcgcca ggttctggaa tgaatcaaac agtgccgata
    11701 gaccggcggc cagaccgttc ttggacggtt cgccgacgat cagttcgatc tgcgagaggg
    11761 tcgcctcctc tgcctcctgc cggctcaggg cctgatgcgc gtctctgaac tggctgtcca
    11821 gcagcagatc tcggattctg gtaatatctt tgatatcaac gcccgttccc agcgaaccga
    11881 ccgaacctaa cgaaaacggt acagcggggg tcagatctac gcgctgcctc gtaaaacccg
    11941 gggtattggc gttcgcaatg ttgtggccgg tggtttggat cgcgacttgc tgcgcctgaa
    12001 gcgcctttct ggccatggta agggatgcct ggagactaat catgcgtcgc tcaccttatg
    12061 cttggctgtt cagtactgaa agggacggcg ccggcgacac gatactcccg tccccttgat
    12121 aaagcggaac aggggtcact gccgacgtga gaaacgacaa cgagcccttg atatacgcaa
    12181 ccgttcgatc cagcagcgcc gcattggagt cgttttcttc taccagcctg gtcacaagaa
    12241 cagccagtcg gttcagaagc cttcgataag acatcgtatg tgaggcgggt accagatccg
    12301 caagacgaaa tagcgcgagc tccgccaatg ggatgtcgta tgtggcgctg accctggtca
    12361 tcagcgcgcc tcgggcctcc tgaatgatct tcagttcgag cgccagtgtc tcttttcgct
    12421 tcatcagctc ggccagggcc ccaaggtcgc ccttgacgat cagacctctc tcctgacgaa
    12481 ggagtcgaag tagcgcctca tacttctcgg tctcttgctc gagtacttga gccaaatctt
    12541 ccagcagcat catcatcgtc tgagacacca tttgtaaggc tgaaggtaga taggctgaag
    12601 gtggaacgtt tggactgctt cagccttcgg tactcggtct aacccctgtt tgtaagcctg
    12661 atggctacac aagatgatcg aacagtccct gtccgatcat cttcgctgcg acctcgcgac
    12721 ccttgacgtt atacacccct cgctggatca ttcccttaag ctccgccacc ttttcggggc
    12781 gaacctcagg cgaggccgcc aggagcgttc gagcctgctg aagcgccttc gccgcctgcg
    12841 aaagctcgac gcgatcggca ggcgcatcat gagccggggc cgactgtttc gattctggtc
    12901 gctccgccgc gtcctggact cggttaagac aggggttaat attcgcactg tcgccacggt
    12961 tctcgatttt caccggccta ctccttctac aagcaacctt caggtatcag cggtcagcta
    13021 atagccgatc agtgatcgct attagcggtc tctctttgtg atatcggtag attgggaagg
    13081 ttccttgagt cgattttcgt agactccagc cctcagggtc gcccgcagtt gctgttccag
    13141 catcttcccc acaccgatcc cgccgcttcg ggcgatcgcc ttcgcgatct catccgtaaa
    13201 caactgccga tagaggtcct gcgacagccc accgccaaac agcccggcct gctcactcgg
    13261 ccgcgcctcc ttgaggagcg aggcgatcag gattgcttca aacccttggg ccacttctct
    13321 gagcttctgc tcttccggcc gcgtacgctg ttgcacggcg tgcagtccct gcgatacctt
    13381 gcccggatcc ataccatcct ccaaccggca ttacataatt tgcagttcgc cctgcaaggc
    13441 gcctgcggcc ttgatcgcct gcagcaggga aatcatgtcc cgcggcgtga ccgccagcgc
    13501 attgagcgcc ttgatcacat ccccgatact gacgccctca ctcataaggt tgacgcgggt
    13561 cctgtcttct ctcgcctcta tctcggtcct aggcacgacg gtcgtcttgc cttcggacag
    13621 cggggccggc tgggagacca gcagttccga ccggatctgc aggctcaggt tgctgtgggc
    13681 gacagccacc gtcgagatgc gcacctgact acccataatg atcgttcccg tccgttcatt
    13741 gacgaccacc tttgccgtca tatcaggcac caggctcagg ttctcaatag ccgcaatcag
    13801 ttccaccgga ttggacagat acgagtcggg aacccggatc tcgaccgtgg acgcatcctt
    13861 cgccctggtt tccgctccaa gtgagtgccc gactgcctga gccattcgga tggcagtggt
    13921 aaagtccgga ttggtaagca caagcagcaa ttgctgggcc ttcaggaaat ccgcggacgg
    13981 ctcccgctcc acgatcgccc cgctgggaac tctacccaca gtcggatggt tcttctggac
    14041 cttttcgccc gtccctcccg cctcaaagtt gaatcctccg atagaaatcg gaccctgcgc
    14101 gacggcgtag acctgaccgt cggcagcctg aaggggggta atcaacagca tgccaccctg
    14161 caaattggtc gcatccccca tcgacgagac cgtcacgtcc agggtgctgc ccgctttggc
    14221 gaagggcggc agcttcgctg tcaccataac cgccgccacg tttttcagcg tcagacgctc
    14281 agccggcacc gtaatcccga gttttttgag catactcacg acggcctgga tcgtgaaggt
    14341 gacctgcgcc ctgtctccgg tcccattgag tccgaccacc aggccgtatc caaacagctc
    14401 gttttctcga acgccctgaa tcttagcgat atccttaatt ctcgcctcgg ccatggcagg
    14461 agccggcaag agtgccgtca cggcgcccat aacggcaacg aaagcaacaa ctttcgccag
    14521 tgcgctacgg agggcggaag gctgaaggct gaaggctgaa ggaatgaggg tgtagggggt
    14581 agggggtcgg gtaacgggtc ggaaagaaca gatcccttcg acccttctct ccctccaccc
    14641 tataccctgt accctatacc ctgtcacttt gcacctcgct cagtagagga acagccagtc
    14701 ggcaaagcgg ttcaggagac ctgggcgctg cttctcggcg atggcaccct tcccctccag
    14761 gacaatctcc gcgtcggcga tctgtgtcga taaaatggta ttggcgggag taatgtctac
    14821 ctggcggatt accccactga ttgctacata ttggctttca tcgttggccc gaaccgctcg
    14881 acgcccctca atgatgagat taccgctcgg aagcactttg accactcgcg ctacgactct
    14941 tgccgtcact tcatcactcc gggtgataga gcccgacccc ttatgctcat tcgccccctc
    15001 aaaggcatat tgtgtcttat cccccaatgg tagaccaaag aggctcgaca ggttgccaga
    15061 gttcgtagac tccttcttgg tgttggtctc agcctgacga ttgagactac tcgattctgt
    15121 cacgagaacg gtgacgatgt cgcccacgtg ctgcgctttg gtatcgacaa agaggaaacc
    15181 ggaaccgatg tcccgccata acgattcccc ggaagcagtt ccgccgcaca gccagagcac
    15241 cgccgccaac cccgcacacc atcgtttcat gcggatgccc ccgctctctt cgctgtcagc
    15301 tttaaccgcc agcggatcga ttgtggacta ctgatcgcta atggccgcat tattcagacc
    15361 cgatccggat cgtcttgtcg ctttctagct tcccgaggac ctctcggcca gaggctgtat
    15421 ttttcacccg aacaagctgg ccggctttgc caccctcctg ggcaatcccc ttcgccgtaa
    15481 ccagcaagcg tggagtctcg accattagag tgacaacttc ccctttctgg atgaggggcg
    15541 gcagctccac cgattcggag accacaacct ctcccatagc caacatccga gtcgtccgac
    15601 ggcctacggc cagcgtgagg tcttgaagcg gctcgtgagc gagcgggccg atgtcccggc
    15661 gctcaagccg cacatcggtc cccttcacca ccgtatgacg gggcaaagga taattcacca
    15721 ccacaacctc gcgtataagc gacagcctca cggcgagcga cacagcccgg tagagccgtc
    15781 catcgatcca tacttccaca ataacaggaa tcgacccgag gccaaccgac gactggaaac
    15841 gcggtctagc cctcagctcg atctcgccgg gcggcaccac gagggcggag gggagcgtat
    15901 cgggacgaac cgtgatccgt tccagttcct gttctcgccc tttgaggacc gccgacttag
    15961 cggcctggaa caactcatca ctctctatca cgcgatgcgc gcccgccacc acgacgtggc
    16021 gaggcgactc gatccggatg ccggacggat cgatcttata ttgacgaagc ttgatcacga
    16081 tcaagtcggg atcaaacatc cgagtgagac caggcggcgg cgcctgtccg accggaaggg
    16141 cttcgagcgc ttctgacagc gactgactct gcgcggtaac ctcggcaaga tccttgagga
    16201 gaatctcctt gccccgtacc gtcgccgact ctttcactcg aatgaccgca cgaggcacgc
    16261 gttcacgctg ctccagcggg aggccctggc cagccagcgc ggaccgcaca acagcgatca
    16321 cgatcgccag cactacaccc gctacctgga ttgccctggg taggcggaag agaactccgc
    16381 ttaatttcac cgtacatccc tgtagggttc gaatcggcgc tggcaggctg cgctgtccgt
    16441 gcttcgcctg ccatcgacga cgcctcgtca cctgccgcct accctccatt ctgtacatcc
    16501 cgccccctat accctatagc ctactacttc cttatctcct gatattattc gagacctgga
    16561 gcatctcatc ggccgcctga accgcctttg atcccgcctc ataagcccgc tggctcatga
    16621 tgaggttgac catctcctcc accactttga cgtttgacaa ctccaggaag ccctggccga
    16681 ttgtccccag accctgagtc ccgggagcgc cactggtcgg gtcgccggag gccgaggtcg
    16741 gcagcaagag gttacggccc acgctctgca gaccggccgg attgatgaaa cggctgagtt
    16801 cgatcgtccc gatctgctgg ctcgccgtct gcccgggaat ggttgccgac accacaccgt
    16861 cagagccgac atggatcgtc atcgcctgcg caggaatgga cacctcgggc tgcaacgcga
    16921 agccgtcgga tgtcacaacc cgcccgttgg cgtcgcgctt gaagctgccg gcacgggtat
    16981 aggcgatcgt cccatcgggc cgcaggacct gaaagaaccc gtcaccttcg atcatcagat
    17041 cgagcggatt ttcggtctgc tgcggatcgc cctggatgta gatccgctgg ctcgctatcg
    17101 gcttgctccc atgcccgacc tggataccga ccgggatctc ggcgccactt gccgtagaag
    17161 cgccggcggg ccgatatgtc tggtaaaaca ggtcctgaaa atcgaggcga ctccgcttga
    17221 acccggtcgt attgacgtta gccaggttat ttgcgatgtt gtccaggttg agttgctgag
    17281 cctgcatccc ggctacggcg ctgaacaatg ctcggatcat ctgcgctcct ttttctgtta
    17341 gattctgccc aactcagagg ccgcctggtt caggctctgg tccagcgcat ggatcgcctt
    17401 ctgataggcc tccgacgcgc gtagggagac cataatctgc gtcatagcgg tgacggcatc
    17461 aacgtttgac ccctccagga atccctggcg gatgtcgacc gctttcgcga cagggggctc
    17521 cggagcgctc ggcttgaggg taaagaggct acctccccct cggataaggg tggtagagtc
    17581 cggatcgacg attcgcaggc gatcaacctc ctgtccttcc accagaatcc gcccaccgtt
    17641 gtccacaata aaccgcccgc caggaatctg caccgcccct ttcttaccct gaacgcggct
    17701 gccatccagg gtctccagat gtccgtcggc atcgatagcg aaattgccag cccgcgtgta
    17761 gcgcactcct tgcggcgtct gtacgacaaa gtagccgggg ccgctgttgg ccaggtccag
    17821 cggattctga gtctcacgga tcaggcctcg accgaaatcg gtcacgcggc gaccaatccg
    17881 gaccggctga ggaccttccg gccgcccctc gatgagcagg gcggattgga gcggtcctct
    17941 ggcgcctcct tcgatgacca ggaggtcacc tttgaaccca ggcgttcccg cattggcgag
    18001 attctgcgac agcgcctcca tcttcgccat ctgcgccatc agaccggacg ctgccgtata
    18061 cagtccactc accatcagat cgcctccttt cgcgcactct cactccgcgc ctacccgagg
    18121 agagacagca agccgtgtgc caccgccatt ccatggaaaa gaagggcaat ctcagagaaa
    18181 aagggagtga ttcaggacag gacgactgga gatggggaaa aatttgccgc agtacccgag
    18241 ccatcggcat aggcgcagtc caccacgagc cgacgatcga caagatagac actacccggt
    18301 ttggccttcg cggcagtctt cagttcggcg gcgcgggaaa cgagctgacc cacatgttca
    18361 aagccccgcc cctcgctcgg aacgacggca atagagagcg tcataagcgg aaagcggcag
    18421 aggcgattcc ggcggtcctt cagaagcaga gatccctgac gacgatcagt ctcatcatag
    18481 agggaaggcg cgatcagatc gaaattcgcg acaatctccc gacaggccga ttcgatctgc
    18541 tccatcggca ggatcacaac aaaatcatcc cctccgatat gtccgacaaa gccggatccc
    18601 gaccccaggt tcataacagc gtttaagatg attcgtgccg tcatcctgag tgcttcatcg
    18661 cccctggcaa agccgtaccg atcgttgtac gccttgaacg agtcaatgtc gagataggcc
    18721 aacgcaaagg cctgatcggc gtcaatccgc cgttgaattt cttgaagaat ggtgttgttt
    18781 ccgggtaagc gggtcagcgg gttggcatcg agttcgagat ttgatcgcag gaggcagaga
    18841 cgactcctga tgcacacctc ttgcgggctg acgggcgccg caacaaaatc gctaagctga
    18901 agctccgccc acgtatcgac ccgctcgtcg gccccggtct ggagcagcag gatcatcgga
    18961 agctggcgga acaccggatc ggcccggatg agcctcgcaa tcctctctcc ctccgaggcg
    19021 gacagatcat cgctcagaat catgagatcc gggggatcgg catacatctc gtacagcgcc
    19081 tggcaaggat tgcttggcgt cttcacctcg aagccttcgc acatcagacg ctcgaccgcc
    19141 tcatgaggca gagagggatc gactcgaatg aacagaatct ttgcgctgtg gtccgtcatg
    19201 ccgctgaata ctcaggcaaa ggtcgcggta atctcttgct cgtcaacctc actcagcgcc
    19261 tcgtcaacct gtcccatcgt caagtcaaga aggttccatg ccgtatgtga gagcggggga
    19321 accttcgcat cccccgaatc accgatactc gccgcaatac agagaatatc ggccagatgg
    19381 acaatggcgg tggtcagcgt cgcctcccgg gccagatggg gaacgtgatg cagcgcgatc
    19441 ggctccacca gctttaccgg caacttccag tgttcaagca accatcgacc gacctcagca
    19501 tgggtcgtac ccaggacttc catctcggcc tcgatcatga ggagatttcg cttcttggcc
    19561 agcccaaccg ttgcggtaaa ctcttccggt agatcagcgc acaggacgat ctttcccaag
    19621 tcgtgcagca gcccggccac gaccgcctcc tcaggctcgt ggagccccgc tttcctggcc
    19681 agaacgcccg cgatccgtgc ggtcccgatc gcgtgatccc acagatgctt gcgatccaga
    19741 ccgtcccctc gtccctgctg aaacaggtcg aaaacggacg cgctcatgac aagccccttg
    19801 accacattaa acccaagaag tacggtcgcg tgcgtgaccg tcgacacccg ccgggaaaaa
    19861 ccgtagaaag cggaattcac cagcttgagg accctggccg agagaacctg atctttcgcg
    19921 atgacccggc ctacatcggc gacagaggtc tccgggct
//
LOCUS       DATMXI010000008         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_63797, whole genome shotgun sequence.
ACCESSION   DATMXI010000008 DATMXI010000000
VERSION     DATMXI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63797"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="VN444_00225"
     CDS             <1..1843
                     /locus_tag="VN444_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR20269.1"
                     /translation="EGNVRLWSQAAERIFGWRKEEILGGLLLTVPPDKREEYQALRER
                     ALAGETLAGVEVVRQTKDGSSVDISLWTAPLRDEGGGISGIVGIMADITKRKQAEGRI
                     RLQSAALEAAANSIIITDRGGMILWVNPAFTRLTGYTAEEAIDRSPGILRSGRHDRAF
                     YHNLWETILAGRVWQGEIVNRRKDGSLYTEEQTITPVLDGRGEITHFIAIKQDITERT
                     LLEDQLRQAQKLEAVGLLAGGIAHDFNNHLNGIIGFAELTLGGIPPDSKEYRHLSRVP
                     KIGRQAAELVSQMLNFARKAPLHRKPLDLDLLLQETMSVLRRTLPETIAIELEPARES
                     LVVNADGAQVQQILLNLATNARDAMPHGGTLTLRLTPVTLIETSLGGHPERRLGAFAC
                     LTVADTGTGIPAAIRDRIFEPFFTTKEPGHGTGLGLASVYGIARQHEGWIEVETAEGQ
                     GSAFQVFLPLVPTAVKAASLVEKALPRGTETLLLVEDNPMVLELGELLLSNLGYTVLS
                     AADGVEALATFQAHSDIALVLTDAIMPRMGAADLIPALRALNPDVKVLVATGYAPDEI
                     HHSLESLEVTGYVRKPFSQADLATAVRAALDGPADAGRPKGESGATV"
     gene            1944..2126
                     /locus_tag="VN444_00230"
     CDS             1944..2126
                     /locus_tag="VN444_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20270.1"
                     /translation="MEQQFVAITLHRIAGKLVCGVVTLTRQPDRAWCGKCQKCGEEFR
                     VEPDARFEGQVRAMRN"
     gene            2130..>2470
                     /locus_tag="VN444_00235"
     CDS             2130..>2470
                     /locus_tag="VN444_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HWR20271.1"
                     /translation="MLNLELKARCEDIGKLRERCESLGAEGHEPERQVDTYFNVNHGR
                     LKLRESLESGAELIYYIRDDVAGAQESHYELYRVEDAEGLKAMLAKALGVSIVVAKRR
                     ETFVLDNIRIHL"
BASE COUNT          498 a          724 c          795 g          453 t
ORIGIN      
        1 tgaggggaac gtcaggctgt ggagccaggc agcggaacgc atattcggct ggcgaaaaga
       61 agagatcctt ggaggcctgc tgctcaccgt tccacccgac aagcgcgagg agtaccaggc
      121 gcttcgcgag cgagcgttgg caggggaaac gctcgccggc gtcgaggtcg ttcgccagac
      181 aaaggacggt tcttcggtcg atatcagcct gtggacggcg ccgctacgcg atgagggggg
      241 cggcatcagc ggtatcgtcg ggatcatggc ggatatcacc aagcgcaagc aggccgaggg
      301 gcgaatccgt cttcagtcgg cggcgctgga agcggccgcg aacagtatca tcattactga
      361 tcgcggcggc atgatcctgt gggtcaaccc ggcctttacg cggctgaccg gctataccgc
      421 cgaggaggcc atcgaccgga gcccgggcat cctcaggtcg ggccgacacg atcgggcgtt
      481 ctaccacaat ctgtgggaga ctatccttgc cgggagggtg tggcagggcg aaatagttaa
      541 tcgccgcaag gatggtagtc tttacactga agagcaaacc atcacccccg ttctagacgg
      601 acgaggggaa attactcatt tcatcgccat caaacaggat atcaccgagc gcacactcct
      661 cgaagatcaa cttcgccaag cgcagaagct ggaggccgtc ggcctcctgg ccggggggat
      721 cgcgcacgac ttcaataatc atttaaacgg gattatcggc ttcgccgaac tgactctggg
      781 gggaataccc ccggatagca aggaataccg tcaccttagt cgggtaccga agataggtcg
      841 ccaagccgcg gagcttgtaa gccagatgtt gaactttgcg cggaaggccc cgctgcaccg
      901 gaaaccgctg gacctggacc tgctactcca agagacgatg agtgtcttgc ggcgaacgct
      961 tcccgagacg atcgcgatcg aattggagcc cgcccgtgag tccctcgttg tcaatgccga
     1021 tggtgcccag gtgcagcaga tcctgctcaa ccttgccacc aacgcccgtg atgctatgcc
     1081 ccacgggggc accttgaccc ttcggctgac gccggtcaca cttatcgaga cgagtctggg
     1141 cggccatccg gagcgccggc tgggcgcctt cgcctgcctg accgtagccg ataccgggac
     1201 cggcatcccg gccgccatcc gggatcgcat cttcgagccc ttcttcacga ctaaggagcc
     1261 tggccatggg accggcttgg gcttggcttc agtctacggg atcgctcgcc agcacgaggg
     1321 ctggatcgag gtggagacgg ctgaaggcca aggcagcgcc ttccaggtct ttcttcccct
     1381 ggttcccact gcggtaaaag ccgcgtcatt ggtcgagaag gcgctgccgc gggggaccga
     1441 gacgctcctg ctggtggagg acaatccgat ggtcctggag ttgggagaac tccttctcag
     1501 caaccttggc tacaccgttc tcagcgctgc ggatggcgtc gaggcgctag cgacctttca
     1561 ggcgcactcc gacatcgccc tggtccttac cgacgccatc atgccccgga tgggggcggc
     1621 ggacctgatc ccggccctgc gcgccctcaa tcccgacgtc aaagtgctgg tcgccaccgg
     1681 ctatgctccg gacgagattc atcacagcct ggaaagcctg gaggttaccg ggtatgttcg
     1741 gaagccgttt tcccaagccg acctggctac tgcggtccgc gcggccctcg acgggccggc
     1801 agatgctggg cgccccaagg gagaatcagg cgcgactgtc tgaggggagc ccgctctctc
     1861 gatcagcaaa cggccttttc cttccgacag atacgataga ttgagataag aaattggaac
     1921 ggcttgatgc tcggaggcaa acagtggaac agcagtttgt tgccatcacg cttcaccgaa
     1981 ttgccggaaa gctggtctgc ggcgtcgtta ctctgaccag gcagccggat cgcgcctggt
     2041 gcggcaagtg ccaaaagtgc ggggaggagt tccgggtgga gccggacgcc cggttcgagg
     2101 ggcaggtccg cgcgatgagg aactgactca tgttgaatct tgaactgaaa gcgcgctgtg
     2161 aggatattgg aaagcttcga gagcggtgcg aatcgcttgg agcggagggt catgagccgg
     2221 agcggcaggt cgatacctat ttcaacgtga accatggccg cctgaagctc cgagagtcgc
     2281 tggagtccgg cgctgagctg atttactata tccgagatga tgttgccgga gcacaggaaa
     2341 gtcactacga gctctatcgc gtcgaggatg cggagggcct caaggcgatg ctggccaagg
     2401 cgctcggcgt cagcatcgtg gttgcgaagc gacgcgagac ctttgtgctc gacaatatcc
     2461 ggatccacct
//
LOCUS       DATMXI010000009         3676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_193801, whole genome shotgun sequence.
ACCESSION   DATMXI010000009 DATMXI010000000
VERSION     DATMXI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193801"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..353
                     /locus_tag="VN444_00240"
     CDS             102..353
                     /locus_tag="VN444_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20272.1"
                     /translation="MPTVGMTVVVVVVVVVVVVVVVVVVVRNTESLSCLAAVAQEKLP
                     VPPEHAALLLPRNPDTAPDPDKVVPGVKRDELFNTSTTH"
     gene            1240..2355
                     /locus_tag="VN444_00245"
     CDS             1240..2355
                     /locus_tag="VN444_00245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20273.1"
                     /translation="MKICVIGPAYPLRGGIAHHTAFLTRELKKRHDVTLLSYTRLYPS
                     LLFPGTTELDTSRLMCAVECEPIFDPLNPRSWLSTARRIRALSPDLVIVQWWSPLFAI
                     PLRAVISLVKRNSRSSTLFICHNVRPHERLPGEGMLLQFGLAPADRFIVHSETDLRHL
                     RAIRPDAAIHKLFLPVREEFSPAVDRQEARRQLSLSGPTILFFGLIRRYKGLPDLLRA
                     MPTILKSMECTLLIVGEFYEGREECLSLIRSLDLAAHVRLVDRYVANEEVGLYFGAAD
                     LVVLPYISATQSSVVTMAYAFDRPVITTRIGGLPEMVTDGRTGLTVDPADPAALADAI
                     LRFFREPQKVASIEGIRSARERFSWQRFVDTVEALVR"
     gene            2516..>3676
                     /locus_tag="VN444_00250"
     CDS             2516..>3676
                     /locus_tag="VN444_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWR20274.1"
                     /translation="MTSRIPHLKDRIARRDYTVGVIGLGYVGLPAVVRFGEVGFRVLG
                     FDIDPHKVKRLTAGESYIRHLPADRIASLSGSGRFEATSDFTRLGEADALLICVPTPL
                     TRHRNPDLRYVTQTADAIARTLRVGQLICLESTTYPGTTEDLLLPRFEAKGLKVGEDF
                     FLVFSPEREDPGNTHFGLATMPKVLGGVSDACGELGEALYTTIVPTVVRVASPKAAEM
                     TKLLENIYRAVNIALVNELKMLSDRMGIDIWEVIAAAATKPFGFQAFYPGPGLGGHCI
                     PIDPFYLTWKAREYGMATRFIELAGEINHAMPTWVVGKVVEALNRRGKSLNGAKVLVL
                     GVAYKPNIDDQRESPALEILTLLRQQGAQVNYSDPHVPRCYGHRHYPDLDLTA"
BASE COUNT          733 a         1126 c         1026 g          791 t
ORIGIN      
        1 gcgcgtatca gtgtgccgac tactgcaggt gaactcttcg agctttcagc atccaggcga
       61 ttgcgcccaa gaaggccaac cctagaacca tcattcccca gttgccaacc gtcgggatga
      121 ctgtcgtcgt agtcgttgtc gtagtcgttg tcgtagtcgt tgtcgtagtc gttgtggtca
      181 ggaatacgga atcgttgtcc tgcttggcgg ctgtggcaca agagaagtta cccgtgccgc
      241 ccgagcacgc tgcgctgctg ttacccagga accctgacac tgcgccagac ccggataaag
      301 tcgtcccggg tgttaagagg gatgagctgt tcaacacgag caccacacac tgaccactgc
      361 ttgtgttgaa gccatccgcc gggaacaacg cacctttagc gaccgacgtc gttggaccag
      421 ccgagaacgc atatcgattc ccggatgcga ctccggcagg aactcctccg cccccgccgc
      481 tgaccgtaaa tcccagatct gaatcaccga agccatcgat tacaagccca ttgagcgtcc
      541 accgccccgc tcctgaatta gcggcagggt cgaaagttcc cggcagagtg cagttcacaa
      601 ggcccgtgtg ctttacactt ccagcagcaa atagcgggct tatggagaca tcggcttgaa
      661 gtgggtcacc gttgaccgac gtcgttgaac cactcacacc gccggtgctg gctcctacgc
      721 cgaccgcgtt cacatggcca ggatcaccta ccgccccatc atttctgaag gcgaaatacc
      781 cggtcccatt atccgacata aaaaagttgg actggaatcc tgcctcgctc atcgggacaa
      841 taacccattg cacaaggaac atagggacta gcaaagaaaa agccaacttc ttgctgaaag
      901 caactcttgt ccttgatctc atccttgctc ctccttgccc cgtcacaggg catgtcgacg
      961 ccaaccgacc actacttcga acgatccgat caatacgcta tccttaaaaa tttcagcttt
     1021 ctcttttgcg ttgactcgcc catttctatc tgagattacc caacaatgac gtcaggcggg
     1081 atgacgccaa cataccaagt aaagataacg tggccattgg taccacacgt tcttttcgct
     1141 tgtcaagttt ttttttgaac gaacgcttga ctcaagacga ccgccttgat acatacacac
     1201 agaccagcgt cgcctcacag tctcagggta tcgtgagtca tgaagatctg cgtgatcgga
     1261 ccggcgtatc cattgcgggg tggcatcgcc caccacaccg ccttcctgac ccgcgaattg
     1321 aagaagcggc atgatgtcac cctgctttcc tatacccgcc tctatccttc gctcctcttt
     1381 ccagggacta ccgaactgga tacaagccgc ctgatgtgtg cggtcgagtg cgaaccgatc
     1441 tttgaccccc tcaatcctcg ctcctggttg tcgacggcac ggcggatcag agcgctttct
     1501 cccgatctgg tcatcgtgca atggtggagc ccgctcttcg cgatcccgct gagggcggtc
     1561 atcagcctgg tgaagcggaa cagccggtcg tcaacgctct tcatctgcca caatgtcaga
     1621 ccacacgaac gcctacccgg cgaggggatg ctcttgcagt tcggccttgc gccggccgac
     1681 cgtttcattg tccattcaga gacggatctg cgccatttgc gcgccattcg ccctgacgcc
     1741 gcgattcaca agctctttct tcctgttcgc gaggagttca gcccagccgt cgatcgacag
     1801 gaggccaggc gacagcttag cctcagcggc cccaccatct tattcttcgg cctgattcga
     1861 cgctacaagg ggcttcctga cctgcttcgg gcgatgccga cgatcctgaa gagtatggag
     1921 tgtacgctcc tgatcgtcgg cgagttttat gagggacgcg aagaatgcct cagcctgatc
     1981 cggtccctgg accttgctgc ccacgtccga ctggtcgatc gatatgtcgc aaacgaagag
     2041 gtcggcctgt atttcggcgc agcggatctg gttgtcctgc cgtatatctc ggccacccag
     2101 agcagcgtcg tcacgatggc ttacgcgttc gatcggccgg tcattacgac gaggatcgga
     2161 gggctgcccg agatggtgac cgatggccgg accggtttga cggtggatcc cgccgacccg
     2221 gcggccctcg ccgatgcgat cctgagattt tttcgcgaac cgcagaaggt ggcttccatt
     2281 gaggggatca ggagcgcccg cgagcgattc tcctggcagc ggtttgtcga taccgtcgag
     2341 gcgctggttc gctgatttcg ccgacagcat gttcgccatt gccaacctca ttgtcggggt
     2401 gctactctga tcgcggtgat tgtctgtcgg gaagaatcag tacacgtgtg acacggtcgt
     2461 ttagccttcg cggcgccggc aatagccgtc gtgtcgtgac gtgaaaggac acacgatgac
     2521 atcgcgcatc ccgcatctga aggatcgcat cgcgcggcga gactacacgg tgggcgtcat
     2581 cggcctgggc tatgtcgggc tgcccgccgt cgtacgcttt ggggaggtcg gtttccgggt
     2641 actggggttc gacatcgacc cccacaaggt caaacggctt accgccggtg agagctatat
     2701 ccggcatctc ccggcagatc ggatcgcctc cctgtccggg tcgggtcggt ttgaagccac
     2761 ctctgacttc acccgccttg gggaggctga cgcgctgctc atctgcgtcc cgacgccgtt
     2821 gacgcgtcat cgaaaccctg acctccggta cgtcacgcag acggcagacg ccattgcccg
     2881 aaccctgcgg gtcggccaac tcatctgtct ggagagcact acctatcccg gaaccactga
     2941 ggaccttctc ctgccgcgct ttgaggcgaa aggactgaag gtgggagagg atttcttttt
     3001 ggtattctcg ccggagcgtg aggacccagg caatacgcac tttgggttgg ccaccatgcc
     3061 caaggtcctc ggcggggtgt cggacgcctg cggcgaactg ggagaggcgc tctacacaac
     3121 gattgtgccc acggtggtcc gggtcgcctc gcccaaggcg gccgaaatga ccaagcttct
     3181 ggagaatatc taccgggcag tcaacatcgc gttggtgaat gagctgaaga tgctctcaga
     3241 ccgcatgggg atcgacatct gggaagtgat cgccgccgca gcgacgaagc ccttcggctt
     3301 tcaggccttc tatcccggac caggtcttgg ggggcactgt atccccatcg atcccttcta
     3361 tcttacctgg aaggcccgcg agtacggtat ggccacccgc tttatcgagc tggccggcga
     3421 gatcaatcac gccatgccta cctgggtggt aggaaaggtc gtcgaagctc tgaacaggag
     3481 gggtaagtcg ctgaacggcg ccaaggttct ggtcctgggg gtggcataca agccgaatat
     3541 tgacgatcaa cgggagagcc cggccctgga gatcctgacg ttgcttcggc aacagggcgc
     3601 tcaggtaaat tactcagatc cccacgtccc gcggtgctac ggtcatcgtc actatcctga
     3661 tctcgatctg accgcc
//
LOCUS       DATMXI010000010         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_87400, whole genome shotgun sequence.
ACCESSION   DATMXI010000010 DATMXI010000000
VERSION     DATMXI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87400"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..543)
                     /locus_tag="VN444_00255"
     CDS             complement(181..543)
                     /locus_tag="VN444_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWR20275.1"
                     /translation="MTHILLYLLLGLVAGIFSGLIGIGGGIIIVPALVFLFGLSQHQA
                     QGTTLALLVPPIGLLAAWTYYKEGYVDLRIASLICLGFFLGGLLGAKLATGLSNSVLE
                     KLFGTALLLMSLKMLLAR"
     gene            complement(982..2001)
                     /locus_tag="VN444_00260"
     CDS             complement(982..2001)
                     /locus_tag="VN444_00260"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWR20276.1"
                     /translation="MEGTAVLSRIRGYKKALGITMWLVIFAFIGTTFLVWGFRSASGP
                     GGQDPLNPIAAVDGEKIPYTEYQQVYQRQYRQYQEKLGDKFDEKILEQLNLKGQIVEA
                     LIGRRLLLHEAKRLDIVISPDELAAEITKMPAFSDATGFNRDRYLRTLQSASLTPERF
                     EEGLREDLLLRKVEEWVKGGIHLLPDEEWEAFRFNRASVKAEYLLFSDPKTQQATIQK
                     VAGLAKDKKSWEEIVRASGLKPHISDFFSWDRDLPQVTDQQSFKEAALAMERGVISPV
                     IQGTKVSYILRVADRKDPSPAEFEREKAQFSHGLLGRKREQVFADWIRQVRARAKVKI
                     ETANL"
     gene            2206..>2579
                     /locus_tag="VN444_00265"
     CDS             2206..>2579
                     /locus_tag="VN444_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWR20277.1"
                     /translation="MPVIFDWFFGMFSSDLAIDLGTANTVIYVKGKGIVLSEPSVVAV
                     KKGTNIVLQVGRDAKEMLGRTPGSIVAIRPLKDGVIADFDITEAMLKHFIIKVHNRKA
                     LVRPRIVVGVPSGITQVEKRAIR"
BASE COUNT          574 a          722 c          668 g          615 t
ORIGIN      
        1 ttcttcgtct caatattgat cacctggcag ttacgggatt tattacgcac tacactggaa
       61 tcaggatgcg acaagtggcg gatggatgat ggcggcacac tctcagcgcg gggaagagca
      121 gtacgaggag ggtcacggca caggttggaa ggctcagggc cactccttac gcaccaacag
      181 ttatctggcc agtagcatct tgagcgacat gagaagcaag gctgtcccga acagtttttc
      241 caacactgag ttggacaagc ccgtcgcaag cttggcgccc agcaaccctc ccaagaagaa
      301 acccagacat atcaggcttg ctattctgag gtccacatac ccttctttat aatatgtcca
      361 ggcagccaag aggccgatgg gcggcaccag cagcgccaac gtcgtgcctt gggcctgatg
      421 ctgtgatagc ccgaataaaa agaccagtgc gggcacaatg atgattcctc cgccgatgcc
      481 gatcaggcca ctgaagattc ctgccaccag gcccaagagc agatacagca gaatgtgtgt
      541 catctgtgaa ccttccttgc gttgaggaaa cgttgcgcaa catgcggtca tcgacttcgc
      601 tcgattttgc gatcaggatg ggcagcagca agacgccacc ctcgaatgcc gaatttcgct
      661 aaaaattcta ccgttatcga acacttgaag gcaagcgctt tcgggtcgct tgagattatg
      721 cggcatcgct tcctgttcga tctttctggt ggccaatccg ttggtcaact gcttgacttg
      781 cgacagatct aaggttgaca tcgtattgct tgagaaacct tgaggcaatc gtggctgggg
      841 ccgaggtatc accacatacg gggaaaaaga cggtgattgc gaagaggagg aatgttatct
      901 gctgctaccg cgtcgagggg aggtgtttcg cttcagacac ctcaggatgc gatcgtaacc
      961 cgctcggtta ccatgccgcg ctcagaggtt agccgtctcg atcttcacct tggcccgcgc
     1021 ccgcacttgg cgaatccagt cggcaaacac ctgctcccgc ttccttccca aaagcccatg
     1081 gctgaattga gctttctcgc gttcgaattc tgcgggactt gggtcttttc gatcggcaac
     1141 ccggaggata tagctcacct tcgtaccctg gatgaccggg ctgattaccc cccgctccat
     1201 tgccagcgcg gcctctttaa aactctgctg atccgtcact tgcgggaggt cacgatccca
     1261 tgagaagaaa tcgctgatat gcggcttgag cccggacgcc ctgacgatct cctcccagga
     1321 ttttttgtcc ttggccagtc cggcaacttt ctgaatggtg gcctgttgtg tcttcggatc
     1381 tgagaacaac aagtactcgg ccttcacgga agctcggtta aagcgaaatg cctcccactc
     1441 ctcatccggg aggagatgaa tccctccctt cacccactct tcaaccttac gaagcagtag
     1501 atcctcacgt aggccctcct cgaacctctc aggggtcaga ctcgcagatt ggagggttcg
     1561 gagataccga tcgcggttga agccggttgc gtcgctgaag gccggcatct ttgtgatctc
     1621 tgcggccagt tcatccggac tgatcacaat gtccaatcgt ttcgcctcat gaagcagtag
     1681 gcgacgtccg atgagtgctt ctacgatctg ccctttcagg ttcagttgtt cgaggatctt
     1741 ttcatcaaac ttatcgccta acttctcctg gtactgccga tactgacgct gatagacctg
     1801 ctgatactct gtgtatggga tcttctcacc atcgacggca gcaataggat tcagggggtc
     1861 ctgcccgcca gggccagacg cggaacggaa tccccagacg aggaaggtgg tcccaatgaa
     1921 ggcaaagatc accagccaca tagtaatgcc tagcgctttt ttatatcctc gtattcgact
     1981 cagcacagca gtgccctcca tcggagcttc caacgccaac acgttcatgt cgtttaatag
     2041 ttttgtatca tatcgtattc tgacaatcgg cgcaacccta tcttatttcc cgatccggtt
     2101 ttcgccctag atacttcttg tatttcacat tcttatatgc tacaaaaagc cctagaatca
     2161 cgtatgtctc gcgtcgccgc gggcgtcaca aattagcata aggagttgcc cgtgatcttc
     2221 gactggtttt tcggtatgtt ctctagtgat ctggccatcg accttgggac cgccaatacg
     2281 gtcatctacg tgaaaggcaa ggggattgtc cttagcgaac cgtcggtggt ggcggtcaaa
     2341 aaaggaacca acatcgtcct gcaggtggga cgtgacgcca aggagatgct cggtcggacc
     2401 cctggtagca ttgtcgctat tcgcccgttg aaggacggcg tcatcgccga cttcgatatt
     2461 actgaggcga tgctcaagca tttcatcatc aaggtccaca accggaaagc gctggttcgc
     2521 cctcggatcg tggtcggcgt gccctccggg atcactcagg tcgagaaacg cgccattcg
//
LOCUS       DATMXI010000011         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_218266, whole genome shotgun sequence.
ACCESSION   DATMXI010000011 DATMXI010000000
VERSION     DATMXI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218266"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VN444_00270"
     CDS             complement(<1..1495)
                     /locus_tag="VN444_00270"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR20278.1"
                     /translation="MIRSSAVIRRSSEPNPPEQFQRWLDRVARPIDFATRDAFAHLPT
                     VKNLNRFVSSQVMQALSDHVYPRAIEAALLQLRGLFLHDQERLPAEDEQRRLREAKVI
                     LGVLRKAAHDPAQAWNRPEPVAVREPVIDPAPSRSWWEQPIRFVKGVGPKRTLLLQRF
                     GIETVEDALWTVPWRYEDRSVMTPIGQLVPGEQASICGTIIRSEAKNARRRRLTILDI
                     VVEDSTGRLQAVFFNQPFLEPLFTVGTSIMLTGRVMAGAQGWVAMRMEVAQYEVIGAA
                     TEAPLHVGRIVPVYHETKGWTSRQMRALMKSLLDAHAAEAQELLPVPLRARYRLLPIQ
                     QAIQDVHFPQPGTDGGQLDRGITPAHRRLAFEELFVLQLALASRQRVMKEEVKQVSFN
                     PKTPLLGKLDRALPFQLTAAQERVIREILSDMTSRRPMNRLVQGDVGSGKTIVAVQAM
                     VLACGSGYQAALMAPTEILAEQHYRTMKGLLEPLGLSVVLVSGGGRVA"
     gene            1691..>2243
                     /locus_tag="VN444_00275"
     CDS             1691..>2243
                     /locus_tag="VN444_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20279.1"
                     /translation="MYRRNIFHILYPVACLIGLFILYQAWFKPTYLPTPAPSPLNDIV
                     ETSPEAPPEPRRDGRAELEQKQARLIDPSVVPKTTHHELLETIRDEIERGNVKSAEAT
                     LTNLPATALTETKTRLYIAVLWNNLGIQLEKAGGTAASMHAFKKASSLDATSPTIQLN
                     LAHAYWELRDPGMTQDFLEQLVAM"
BASE COUNT          494 a          700 c          588 g          461 t
ORIGIN      
        1 ctgcgacacg gccacctccg ctcacgagca ccacggaaag cccgagcggt tccaacagcc
       61 ccttcatcgt tcgatagtgc tgctcggcga gaatttccgt cggcgccatc agtgccgcct
      121 ggtagccaga cccgcaagcc agcaccatcg cctgcaccgc cacgatggtt ttaccggagc
      181 cgacatcacc ctgcaccagg cggttcatcg gcctgcgcga ggtcatgtca gacagaatct
      241 cgcgaatcac tcgctcttgc gcagcagtca attgaaaggg cagcgcccga tccaatttgc
      301 cgaggagcgg cgtcttcgga ttgaacgaca cctgcttcac ctcttccttc atcacccgtt
      361 gccgcgatgc cagtgcgagt tgcagcacaa acagctcttc gaacgccaag cgacgatgcg
      421 caggggtgat accacgatcg agttgtcccc catcagtccc aggctgaggg aaatgcacgt
      481 cctgaatggc ttgttgaatg ggcagcaatc gatagcgggc gcgaagcgga accggcaaca
      541 gctcttgcgc ttccgcggca tgcgcgtcaa gcaagctttt catgagtgca cgcatttgcc
      601 gggacgtcca ccccttggtt tcgtgataca ccggaacgat acgccccacg tgcaacggcg
      661 cttcagttgc ggcgcctatc acttcatatt gcgcaacctc catcctcatg gccacccacc
      721 cctgtgcgcc tgccatcacc cgtccagtca gcatgatgct ggttccaacc gtgaagagtg
      781 gctcgagaaa cggctgattg aagaatacag cctggaggcg cccggttgag tcttccacca
      841 caatatcgag gatcgtgagg cggcgcctgc gggcgttctt cgcctcgctt cggatgatcg
      901 tgccgcagat cgatgcctgc tcaccgggca cgagctgccc gatcggggtc atgaccgaac
      961 gatcctcata gcgccacggt accgtccaca gagcatcttc aaccgtctcg atcccgaacc
     1021 gttgaagcaa caatgtgcgt ttcgggccga ctcccttcac gaaacgaatg ggctgctccc
     1081 accatgaccg cgatggagcg ggatctatga caggctcgcg gaccgcgaca ggctccggtc
     1141 tattccacgc ttgggcagga tcgtgagccg ccttacgcaa gaccccaaga ataactttgg
     1201 cttcccgcag ccgacgctgc tcatcctctg ccggcagccg ttcctgatcg tgaagaaaca
     1261 gcccccgcaa ctggagcaac gccgcttcaa tcgctcgtgg atagacgtga tcggacaggg
     1321 cctgcatgac ctgtgaagag acgaaacgat tcaagttctt gacggtgggt agatgggcga
     1381 aggcatcgcg cgtcgcaaaa tcgatcgggc gagcgacacg gtccagccat cgctgaaact
     1441 gttccggggg gttgggctcg gaagagcgcc gtatcacagc gctggatcgt atcatagctt
     1501 ctcttcctcc tcaacgagca gaactggttc atctggtttg tttagttaat ttggttgttt
     1561 ggttcgtctg gttaatttca ttcaaccaaa caaaccaaat aaccagatca accagtcttg
     1621 ccgattagct gacaatcctt tgtctctcct atttagggtg atacagtaaa tttcgtctga
     1681 agaggcccct atgtaccgtc gcaacatctt tcacattttg tatcctgttg cctgtctgat
     1741 cggactgttc atcctctatc aagcctggtt caaaccgacc tatctaccca cgccggcacc
     1801 gagtccgctc aatgacatcg tggaaacctc accggaggcg cctccggaac cacgccgtga
     1861 tggccgagct gagctggaac agaaacaggc tcgcctgatc gatcccagtg tggttcctaa
     1921 aacgacacat cacgaactat tggagacgat ccgcgacgag attgaaagag gtaacgtcaa
     1981 atccgcggaa gccacactca ccaatctccc ggccactgca ttaaccgaga ccaagacccg
     2041 cctctacatt gccgtgttat ggaacaatct tggcattcag ctggaaaagg cgggcggcac
     2101 cgcagcctcg atgcatgcct tcaagaaggc atcgtcgttg gatgccacga gcccgaccat
     2161 tcagctcaac cttgcccatg cctactggga attgcgcgac ccaggcatga cccaggactt
     2221 cctggaacag ctggttgcca tgg
//
LOCUS       DATMXI010000012         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_106465, whole genome shotgun sequence.
ACCESSION   DATMXI010000012 DATMXI010000000
VERSION     DATMXI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106465"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN444_00280"
     CDS             <1..401
                     /locus_tag="VN444_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HWR20280.1"
                     /translation="QKGLVLCALVGGLSGCGSWMHSDKQSVTIFTNPPEASVIIDDYL
                     HLTAPGTVTLSRKGNHLAQVKKEGFQPTFLKIDRTWSWWVLGDIFGCLVIFSPICIMN
                     DMDQGGYYTFDDKIYLTLDRLTGSEPVPSK"
     gene            592..888
                     /locus_tag="VN444_00285"
     CDS             592..888
                     /locus_tag="VN444_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20281.1"
                     /translation="MTALVLAFILLGANWSSSWGADPPCDKYPSGKQVRCAEIWKELN
                     KEDGPLIAQFGLDQLKRREEGKINAEQHLGENMAFIKQSTEKRLERLRARMDKE"
     gene            complement(893..1399)
                     /locus_tag="VN444_00290"
     CDS             complement(893..1399)
                     /locus_tag="VN444_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWR20282.1"
                     /translation="MMACMRSVLSLCVLGWLALPVAQASESSPPISEQVTITDVQVRM
                     SDHGPVVLLWAEGKAIPIFVDVTVARSIQSALNGEKLSRPLSHDLMHTILEAYGGKVL
                     KTVITLRGSTFYGALTVAVQGQVKTFDSRSSDSIALAVHFKAPIIVGRDLLNSAGPLS
                     GESKAEPL"
     gene            complement(1415..2056)
                     /locus_tag="VN444_00295"
     CDS             complement(1415..2056)
                     /locus_tag="VN444_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR20283.1"
                     /translation="MTQAVIRKTFSVPPLGCNCSIIGDPVTKQAIVVDPGGAHEQIVR
                     EVQKLGLTVTHILHTHAHFDHFLASGEMKKATGATICLHQDDLGLWNNLELQCRMFGV
                     TYSPVPLPDYWLKDEEKLSVGQVSVTALHTPGHTPGSMSFHVPGDNLVLAGDTLFRGS
                     IGRTDLWGGDFDAIERSIRERLYTLEDATIVVTGHGPETEIGQEKETNQFFRA"
BASE COUNT          541 a          626 c          620 g          454 t
ORIGIN      
        1 ggcaaaaagg cctcgtgtta tgtgccctgg tcggagggct ttctggctgc ggaagctgga
       61 tgcattccga caagcagtca gtcactattt ttaccaaccc gcctgaggca tcggtcatta
      121 tcgacgacta cctgcacctc acagcacctg gcacagtcac gctcagtcgg aaagggaacc
      181 acctcgcgca agtgaagaag gagggctttc agcctacttt tctcaagatc gatcgcacct
      241 ggtcctggtg ggtcctcgga gatattttcg gctgccttgt gatcttttca ccaatctgca
      301 tcatgaacga catggaccag ggtggctact acaccttcga cgacaagatc tatctcacgc
      361 tcgatcgcct caccggatca gaaccagtgc catccaaata gcttacaggc cgaccggaca
      421 gactggcata ggcccgtcag gatacggcgg gcgtcgatag cgccacttat tccaacgggc
      481 gaggcattcc atggccgacc cttcatacac atggattgtc tgcaggacaa cttgcgttgt
      541 gttatggtga gccaggcgag aaaggcgatt catgccgcga ggagagggag catgaccgca
      601 ctggtgttgg cattcattct actgggagcg aactggagca gctcatgggg cgccgatccg
      661 ccttgcgaca aatacccgtc ggggaaacag gtacgttgcg ctgagatttg gaaagaactc
      721 aacaaagaag atgggcctct catcgctcag ttcgggctcg atcagctgaa gcggcgcgag
      781 gaaggtaaaa tcaacgccga gcagcacctg ggtgaaaaca tggcgttcat caaacagtcc
      841 acagaaaagc gccttgaacg gcttcgcgcc aggatggaca aggaatagcc cctcacagtg
      901 gttccgcttt cgattccccc gatagaggtc cagccgagtt cagcaaatcc cgtcccacaa
      961 tgatcggcgc cttgaaatga accgccaggg cgatggaatc agacgaccgg ctatcgaacg
     1021 tcttcacttg accctgcacc gcaacagtca aggcaccgta aaaggtactt cccctgagcg
     1081 tgatgacggt ctttaatact ttcccgccgt atgcttccaa aattgtatgc atcaaatcgt
     1141 gggagagggg gcgggagagc ttctcgccat tcaacgcact ttggatcgac cgcgcgactg
     1201 taacgtcgac gaaaatggga atagccttac cttcggccca caagagcacc acaggaccgt
     1261 ggtcggacat gcgaacctgg acatcggtga tcgtgacctg ttccgagatt ggcggtgagg
     1321 attcgctggc ttgtgcgact ggtagcgcaa gccagccgag aacacagaga gagaggacgg
     1381 accgcatgca tgccatcatg gtcacctccg gcggtcaggc acggaagaac tgattcgtct
     1441 ccttttcctg tccgatttcg gtctctgggc catgccctgt cacgacgatc gtggcatcct
     1501 ccaaggtata gagccgttcc cggatggatc gctcaatagc atcaaaatcg ccgccccaga
     1561 gatcggtacg tccgatgctg ccacgaaaga gtgtgtcacc ggccaagacc aaattatcgc
     1621 cggggacatg aaaactcatg gacccggggg tgtggccagg cgtatggaga gcggtgacgg
     1681 aaacctgacc gaccgacagt ttctcctcat ccttgagcca gtagtccggc aaaggaacag
     1741 gactgtaggt gacgccgaac atccggcatt gcaattcaag attgttccac aggccaagat
     1801 catcctgatg cagacagatc gttgcccctg tcgccttctt catctctccg gaagcaagga
     1861 aatggtcgaa gtgggcatgg gtatggagga tatgtgtgac cgtcaatccg agtttctgga
     1921 cctcgcgcac aatctgttca tgggcgccac ccggatcgac cacgatcgcc tgttttgtca
     1981 ccggatctcc gatgatcgag caattgcaac caaggggcgg gacggaaaaa gtttttcgga
     2041 tcacagcctg cgtcatggat gcatgctacc cccatgctcc tcagagcgca agcagcggca
     2101 cttcttcata gaatccgaca tggacaagct ggcgcgaacc gtagataatc ctggaaatca
     2161 gataagcggg agggacctga cgtgcaggac aacacaccat cgaaccaatg gcgcttgccc
     2221 gcattattca tgagcgcttc t
//
LOCUS       DATMXI010000013         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_85743, whole genome shotgun sequence.
ACCESSION   DATMXI010000013 DATMXI010000000
VERSION     DATMXI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85743"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VN444_00300"
     CDS             complement(<1..1009)
                     /locus_tag="VN444_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWR20284.1"
                     /translation="MNAHVILEGLNPRQEEAVLHTEGPLLVLAGAGSGKTRVITRRVA
                     YLISHCGVPPWNILAVTFTNKAAGEMKRRVADLLEHNRPESVDQTHHERSQRDGGSVW
                     VGTFHSTCVRILRKHSAALGLKSSFVIYDEGDQMSLMRDCLRGLGLSDRAVKPRAILS
                     RISRAKNELLSPEEYALQANDHMEERTAKLYIMYQERLEGLQALDFDDLLMSTVRLFE
                     EHHEVLAAYQDLWRYILVDEYQDTNHVQYRLIHLLAGKYGHLCVVGDDDQSIYRWRGA
                     DLNNILDFERDHPGCAVIRLEQNYRSTQRILQGAGAVVAHNSDRKGKTLWTENEVGDT
                     IV"
     gene            1417..2055
                     /locus_tag="VN444_00305"
     CDS             1417..2055
                     /locus_tag="VN444_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20285.1"
                     /translation="MNPESVHSKQKRRRQMTVACITVIVSLVAALAALGTVSEIRNHL
                     CYEHYIYTACSKVQIGESEMRVFAWYPEVKPLNSSSSIPHPNRDALTPSRILYDIPPD
                     EKRSYVSDFPSGKQQWLYWEARLKSHLPPGRSIRFGVRWTTYDTGSNPVAGGLVEATW
                     GADDPAAIIVGPADTDPIRTVGQFPPGHYTIVLKVYGNQLSESEVRGGFNID"
     gene            2261..3262
                     /locus_tag="VN444_00310"
     CDS             2261..3262
                     /locus_tag="VN444_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HWR20286.1"
                     /translation="MKKRDTVVALFPLLSVVLLVGWACSRFSSSPSGTEALPAQTPST
                     TNTDVPRPQWTGPPAGAQYVGSESCLTCHEESGQRFHKTMMGRIMSVNPRTPQEQRVC
                     ESCHGPGGPHVAAEGGAIETLITFKKGAEPVEAQNATCVNGCHEKGEHASWKGSTHEM
                     RGVACVTCHKVMESVSTRYNLAKPTAIEVCSQCHQTRRAQLQRSSHMPLREGKISCSD
                     CHNPHGTVTQGMLREDSVNDNCFKCHAEKRGPFLWEHAPVVESCTNCHEPHGSTNPRL
                     LKVRVPRLCQQCHIETRHPTSPYNPTTGAKQVINRNCVNCHQKIHGSNHPSGFAFTN"
     gene            3297..>3476
                     /locus_tag="VN444_00315"
     CDS             3297..>3476
                     /locus_tag="VN444_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20287.1"
                     /translation="MAAFPGARRIGQALLLAVVLPLAAVASVYAEQAPPEEAPPSWVN
                     WLNTGLPFNFELTGLD"
BASE COUNT          733 a         1020 c          996 g          727 t
ORIGIN      
        1 gtacgatggt gtccccgacc tcattttcgg tccataaggt cttccccttc cgatcgctgt
       61 tgtgggcgac caccgccccc gcgccctgca agatccgctg cgtcgatcga tagttctgtt
      121 ccagccggat caccgcgcag cccggatggt cccgctcgaa gtccaggatg ttgttcaggt
      181 cggcaccgcg ccaccggtag atcgactggt cgtcatcgcc taccacgcag agatgcccgt
      241 acttgccggc cagcagatgg atgaggcgat actggacgtg gttggtgtcc tggtactcgt
      301 ccaccaggat atagcgccag aggtcctggt aggcagccag cacttcgtgg tgctcctcaa
      361 agaggcgcac cgtagacatg aggagatcgt cgaagtcgag cgcctgtagc ccctccaacc
      421 gctcctgata catgatgtac agtttggcgg tccgctcctc catgtggtca ttggcctgca
      481 acgcatactc ctcgggggag aggagttcgt tcttcgcgcg gctgatgcgg gaaaggatcg
      541 cacgaggctt gaccgcgcga tcggataagc ccagcccccg cagacaatcc cgcatcagac
      601 tcatctgatc gccctcgtca tagatgacga aggaactctt caagcctaac gccgcgctat
      661 gcttccgcag aatccgaacg caggtggaat gaaacgttcc gacccagacg ctgccgccat
      721 ccctttgact acgctcgtgg tgagtctggt cgaccgactc agggcggttg tgttccagga
      781 ggtccgccac tcgccgcttc atttcaccgg ccgccttgtt ggtaaacgtg acggccagga
      841 tattccatgg cgggaccccg cagtggctga tcagataggc gacccgcctg gtgatgaccc
      901 tggtcttccc tgatccggcc cccgccagta ccaggagcgg cccctcggtg tgcagcaccg
      961 cctcttcctg tctgggattc aggccctcca agatcacgtg agcgttcact tcagtccgtc
     1021 cctccgtcgg ttagtcgaga tttttcaatg atctcgccct catcataggc gaacccgtcc
     1081 gatgcgacaa gcgatttttc gggcctgaag ccgctttgga gatgggctac agtaccgccc
     1141 ctctttggtc gacaagtaca ctttcgagtc gaaagacatg gctgtatgcg gaggcaagta
     1201 cggaggatgt gaccgagaaa aagaatcgga acggagcatg cgccagggcc cagttccagc
     1261 ttgacatccc ctccgatcag ggggtagctt ttggctaccc tggaggcgca gagggcgcta
     1321 tgcgtgtgaa tagcccacat tcctggccgg aaatctttag atgcagatca gcatggctgt
     1381 gcttaggcgt ctcagcggct gcaaaggaaa gcgtcagtga atcctgaatc tgttcactcc
     1441 aagcagaagc gccggcgcca aatgacggtg gcttgcatta ccgtgatcgt ttctctcgtt
     1501 gccgcgctgg cagcactcgg gactgtatcg gagatcagga accatctttg ttatgagcac
     1561 tacatctaca ctgcttgctc gaaagtccaa atcggcgaat ccgaaatgag agtatttgca
     1621 tggtatccgg aggtgaagcc attaaactcg tcgtcttcca tccctcatcc taaccgggac
     1681 gctttgactc cgagcagaat tctgtacgat attccacctg acgaaaaacg ctcatacgtc
     1741 agcgatttcc cgagcggaaa acaacagtgg ctatactggg aagcgcgact gaagtctcat
     1801 cttcctccag gtcgctcaat caggttcggg gtccgttgga cgacttacga tacgggtagc
     1861 aatcctgttg caggtggtct cgttgaggca acgtggggtg ctgatgaccc agcagccatt
     1921 attgtaggcc ctgccgacac agatccgatt cgcactgtcg gtcaattccc gccgggccac
     1981 tatacgatcg ttctgaaggt gtatggtaat caactatctg agtcagaagt tcgcggcgga
     2041 ttcaatattg actgactgat gcttacgcgt tgcgatgaaa gaacgacgac gaataaatgt
     2101 tggggtagtg gaagctccag cgtacgcagg cgacagcggc cagcgccgca cacaacacag
     2161 gtaaacactt gacaatccat accaacaatc atatagtccc ttcactgctg ggagtcaggg
     2221 tcgcgtttcc attggtgctc caaacctaag gggttcaaag atgaagaagc gtgacactgt
     2281 agtcgcctta tttcctctgc tatctgttgt gctccttgtt ggatgggctt gtagtcgatt
     2341 tagctcgtcg ccctctggaa ccgaagctct ccccgcgcaa accccatcta ccacgaatac
     2401 cgatgtgcct cggcctcagt ggaccggtcc accggcgggc gctcagtatg tggggagtga
     2461 gtcctgcctg acgtgccatg aggagtcggg ccagcgcttt cacaagacga tgatggggcg
     2521 gatcatgtcc gtaaacccgc gcacccccca agagcagcgg gtctgtgagt cgtgtcatgg
     2581 ccctgggggc cctcacgtag ctgcggaggg cggtgccatt gaaacattga ttacgttcaa
     2641 gaagggagca gagccggtcg aggcgcagaa tgccacgtgt gtgaacggct gccatgaaaa
     2701 gggtgagcat gcctcctgga aagggagcac ccacgagatg cgtggcgtgg cctgcgtcac
     2761 ctgtcacaag gtgatggagt cggtgtccac ccgatacaac ctggcgaagc cgaccgcgat
     2821 cgaggtctgc tcgcagtgcc accagacccg ccgggcccag ctccagcgca gctctcacat
     2881 gccactgcgc gagggaaaga tcagttgctc cgattgccac aacccgcatg ggaccgtcac
     2941 gcaggggatg ctccgggaag attcggtcaa tgacaactgc ttcaagtgtc atgccgagaa
     3001 gcgggggccg ttcctctggg aacatgcgcc cgttgtggag agctgcacga actgccacga
     3061 gccgcacggc tccactaatc cgagactcct gaaggtcagg gttccccgcc tctgtcagca
     3121 gtgtcacatt gagacccgac acccaacgag cccatacaat ccgacgaccg gagcaaagca
     3181 ggtcatcaac aggaactgcg tcaactgcca ccagaagatc catggctcca accatccctc
     3241 agggttcgcc ttcacgaact gaggtggcaa tctacatgaa gcggaggagg tgggccatgg
     3301 cagcattccc cggggcaagg cggattggtc aggcactgct gcttgccgta gtcttgccgt
     3361 tggctgccgt cgcatcggtt tacgctgaac aggccccgcc ggaggaggct cccccttctt
     3421 gggtcaactg gcttaacacc gggctgccat ttaattttga gctgacaggg ttggat
//
LOCUS       DATMXI010000014         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_304726, whole genome shotgun sequence.
ACCESSION   DATMXI010000014 DATMXI010000000
VERSION     DATMXI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304726"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1211)
                     /gene="sppA"
                     /locus_tag="VN444_00320"
     CDS             complement(243..1211)
                     /gene="sppA"
                     /locus_tag="VN444_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWR20288.1"
                     /translation="MRKVYSTLCLVCVLAAASLQPGCITVNLIEPSGPIKEMQLSGTG
                     DGKVLLLDLSGVISGQGKDGLVPQPNMLATFKEELTRASKDDKIKAVVVRINSPGGTV
                     TASDILYHELREFKIKRKIPVIASMMDVAASGGYYLAMATDSILVHPSTVTGSIGVIM
                     LTVNARGLLEKVGVEASAITSGPRKDMGSPFRVMTAEERGIFQSVIDSFYQRFLAVVQ
                     EGRPHLSPEQIKKLADGRIYSGDQAKAAGLVDEIGYLDDAIEVAKRKAGLTEARVVTY
                     GHRGEYKNNIYSRLFGASPGIAGLADIDVLSMVRGGTPQFMYLWMP"
     gene            complement(1204..>2031)
                     /locus_tag="VN444_00325"
     CDS             complement(1204..>2031)
                     /locus_tag="VN444_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HWR20289.1"
                     /translation="LTRHLREHGSQQGLITHGGGDSRLAVEKAQAAPSIMGRDLATEV
                     TCDRAYQWVESAGQWALSRNDRAGIGATRRWHVVAYDFGVKLNILRRLVDVGCDVTVV
                     PASTTAEQVEALKPDGIFLSNGPGDPEGVPYAVEAVRRLIGYRPIFGICLGHQILGLA
                     LGLKTYKLKFGHHGANHPVTDLRTRKVEITSQNHNFAVQVVSPINRIPDHLPLIDTVY
                     GQVAVTHLSLNDHSIEGLACADRPVFSVQYHPEASPGPHDSAYLFTQFTQLMEKQHA"
BASE COUNT          459 a          635 c          506 g          431 t
ORIGIN      
        1 cacctgatcg cgggccgtgc caggcgcccg gtaacaaccg gtcaagctgc cgaacagact
       61 catggccgtc ccaatcccga gggccgcaca gaggcccacc gccccctgcg cgcccagagc
      121 cagcatcgcc cgacgtccga ccgcggtcgc gaagacgttg cccatcgcgc gatctgctga
      181 ttgagtcatt gcagtcctac ttcggcaaat gaatcgctcc cgcactcaat gcgaaactcc
      241 tgtcagggca tccaaagata catgaactgc ggcgttccac cccgcaccat cgacaataca
      301 tcaatatcgg ctaaccccgc gatgccagga ctcgccccga agaggcggga atagatatta
      361 ttcttatatt ctccccgatg cccataggtc accacccgcg cctccgtcaa accagccttc
      421 ctcttggcca cctcgatcgc gtcatcaaga tacccgattt catcgacgag gcctgcagct
      481 ttggcctgat ctccagaata gatccgccca tcggccagct tcttaatttg ttcaggggac
      541 aggtgggggc gtccttcctg taccactgcc aaaaaccgtt gatagaacga gtcaatcacg
      601 ctttgaaaga tccctcgctc ctcagcagtc atgacccgaa agggtgagcc catgtccttg
      661 cgcggtcctg acgtgatagc gctggcttcg actcccactt tttcaagcaa gcccctggca
      721 ttgaccgtca acataatgac accgatactg ccggtcacag tggagggatg aaccaaaata
      781 ctgtctgtcg ccatggcgag gtagtagcct cctgatgccg ccacatccat catcgaggca
      841 atcacgggaa tctttctctt gattttgaat tcccgcaact cgtgatacag gatgtcagag
      901 gcggtcacgg ttccgcctgg actgttgatg cgaacgacta cggctttgat cttgtcgtcc
      961 ttcgaagccc tcgtcagctc ttccttgaac gtggccaaca tattcggctg cggaacaaga
     1021 ccatccttac cttgtccact aatcacaccc gacagatcca gcaacaacac ttttccatcg
     1081 cccgtgccgc tgagttgcat ttctttgatc ggccccgatg gttcgatcag gttgaccgta
     1141 atgcacccgg gctgcaggct ggcggctgca aggacgcaga ctaggcagag tgtcgaataa
     1201 accttacgca tgctgtttct ccatcaattg agtaaactga gtgaataaat aggcagagtc
     1261 gtggggaccg ggagacgcct ccgggtgata ctgcaccgaa aacaccggac gatcagcaca
     1321 ggccaaccct tcgatcgagt gatcgtttag actgagatgt gtcaccgcaa cctgaccgta
     1381 gaccgtgtcg atcaggggaa ggtgatccgg aatcctgttg attggagaga cgacctgtac
     1441 cgcgaaattg tggttttgcg acgtaatctc gacttttctt gtacgaagat ccgtgactgg
     1501 atgatttgcc ccgtggtggc cgaactttaa tttgtaggtt ttaagaccca gcgcgagacc
     1561 gagaatctga tgccctaaac agatgccaaa gatcggacgg tacccgatta accggcgcac
     1621 tgcctcaact gcgtagggca ctccctccgg atcccccgga ccgttcgaca gaaaaattcc
     1681 atcaggtttc agggcttcga cttgctccgc agtcgtcgaa gccggcacaa cagtcacatc
     1741 acacccaaca tcgacaagcc ggcgaagaat attcagtttc accccgaaat cataggcaac
     1801 gacatgccaa cgcctggtcg cgccaatccc agccctgtcg ttacgcgaga gagcccactg
     1861 ccctgcactc tccacccatt gatatgccct gtcacaagta acctccgtcg ccaagtcccg
     1921 gcccatgatg ctgggagctg cctgtgcctt ctcgacggct aggcgtgaat cccctccccc
     1981 gtgggtgatc agaccctgct gtgagccatg ttcgcgcagg tgcctggtga g
//
LOCUS       DATMXI010000015         5531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_171271, whole genome shotgun sequence.
ACCESSION   DATMXI010000015 DATMXI010000000
VERSION     DATMXI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171271"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VN444_00330"
     CDS             <1..262
                     /locus_tag="VN444_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWR20290.1"
                     /translation="ANIVPRDVSDLIRTFMNGDWKRAREIHLKLFPLCQAMFCETNPI
                     PVKTAMALLGMVGGELRLPLWPISEINLNRLKTAMRAYGLLS"
     gene            315..1118
                     /gene="dapB"
                     /locus_tag="VN444_00335"
     CDS             315..1118
                     /gene="dapB"
                     /locus_tag="VN444_00335"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWR20291.1"
                     /translation="MIRAIVCGAAGRMGGRIIAMIHEADDFILAGAVERPGNPRIGQD
                     AGEVAGIGKIGIPLVGEMGSVVGDGQVVIDFTAPQAAMTHLAISAQAKVPAVVGTTGF
                     GAADLNRIKDLSSAAPCVLSPNMSIGVNVLLKVLAQVARTLGDEYDVEIIETHHRFKK
                     DAPSGTALKMAQTIAEALGRDLDKTGIYGRKGLVGERGKEEIAIHSLRAGDVIGDHTV
                     IFGGMGERIEMTHRAHTRDNFARGALCAARWILGQPPGLYDMHDVLGLR"
     gene            1190..1951
                     /locus_tag="VN444_00340"
     CDS             1190..1951
                     /locus_tag="VN444_00340"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWR20292.1"
                     /translation="MRIVRFVAGKQPRYGVVEGTVVREIEGDIFGKFTVTDRTHSLKR
                     IRFLAPTEPSKIVGVGLNYRDHAEEMKLQTPDEPIIFLKPPSATLPHRGRITYPKLCR
                     RLDYEAELGIVIKRTARNVPVEKAHRYILGYVCFNDVTARDLQHMDGQWTRSKSFDTF
                     APFGPWVETDLDLSDAPIRALLNGVIRQESNINNLIFEVPYLIHFISTVMTLYPGDVI
                     ATGTPSGVSPMRPGDTIEIEVEGIGRLSNTIVAQS"
     gene            1993..3174
                     /locus_tag="VN444_00345"
     CDS             1993..3174
                     /locus_tag="VN444_00345"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWR20293.1"
                     /translation="MGQPFSVAQRLSKLPPYLFAEIDRLKQDAIRRGMDIINLGVGDP
                     DLPTPSHIIARMQEASADPRNHQYPSYEGMLSFRQAVADWYSKRFGVTLDPQTEVLSL
                     IGSKEGIGHIPLAFIDPGDVVLVPDPGYPVYQAGTVFADGIPYFMPLTRERSFLPDLE
                     AIPPEVLNKARLMFLNYPNNPTAAVASRAFFAEAVAFARRHRLILCHDAAYSEMAYDG
                     YLPESLLAVEGAKDVAIEYHSLSKTYNMTGWRIGFAVGCREVLSGLGRIKTNLDSGVF
                     QAVQEAAIAALKGPQECVEAMRAVYKERRDALVDGLSALGFTVDRPQATFYVWIGVPK
                     GHTSASLASALLSETGIVMTPGIGFGRSGEGYIRAALTVDVSRIKEAVERIAASNLKP
                     Q"
     gene            3228..3755
                     /gene="folK"
                     /locus_tag="VN444_00350"
     CDS             3228..3755
                     /gene="folK"
                     /locus_tag="VN444_00350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWR20294.1"
                     /translation="MSERAYIGIGSNLGDRIQRCQETIGAISDIAGVTAIRTSSLYET
                     APVPPASGGWFVNGVVSVQTQIKPGALLLELLRIEQSMGRAAERARGEDRSIDLDLLF
                     VGSQIVEQPDFVLPHPRLHQRRFVLVPLCELDPDFRHPVFGVTMRQLLDRLDDPSLVR
                     LLVPAARYTGSGENS"
     gene            3756..4412
                     /locus_tag="VN444_00355"
     CDS             3756..4412
                     /locus_tag="VN444_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWR20295.1"
                     /translation="MTDRASKPRYIVVEGPIGVGKTSLAELLAERLQARKSLEGPDEN
                     PFIAQFYADMRRYAFQAQLYFLLNRFRQQQELAQFDLFKQSLVSDYLFAKDKIFAYLT
                     LDDNELALYERLQPLLETRVVKPDLVLYLQASTDVLVQRIQSRARVSERELGQAYLED
                     VNAAYNHFFFHYSATPLLVVNTNEIDFVKHKEDFEDLVKQVETVRAGTQYYVSLGSRK
                     "
     gene            4586..5428
                     /gene="panB"
                     /locus_tag="VN444_00360"
     CDS             4586..5428
                     /gene="panB"
                     /locus_tag="VN444_00360"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWR20296.1"
                     /translation="MTSQTVRTVTLQEMKREGRKITMLTAYDYPLALLVDRAGIDLIL
                     VGDSGGMTVLGYETTIPVTMDEMLMMTRAVTRAVKRSMVVADMPFMSYEAEPAEAVRN
                     AGRFVKEGLAHAVKVERGWPSLPCVRAIVDAGIPVMGHVGLTPQTAVLQEGLKVQGRG
                     LNDARRILEDALALEKAGVFAVVLEAIPSVLARVITRRLTVPTIGIGAGPDCDGQVLV
                     IHDLLGLFDRFVPKFTKQYADLAKVISDAVSRFREEVIECRFPDAAHTYSIDQETERA
                     LLEL"
BASE COUNT         1130 a         1519 c         1717 g         1165 t
ORIGIN      
        1 cgccaacatc gtgccacgtg acgtgtctga cctcatccgc acattcatga acggtgactg
       61 gaagcgcgcc agggaaatcc acctcaagct cttcccgctc tgtcaggcga tgttttgcga
      121 gaccaatccg atcccggtca agactgccat ggcgcttcta gggatggtcg gcggagaact
      181 tcgcctgccg ctctggccga taagcgagat caacctgaac cggctcaaaa ccgcaatgcg
      241 agcctacggt ctgctctcat gagtattcct tcagccttca gccttcagcc ttcagccttg
      301 ttcggtgtct ctcgatgata cgagccattg tctgtggtgc ggctgggcgg atgggcgggc
      361 gaatcatcgc catgatccac gaagcggacg acttcatcct ggccggggcg gtagaacgac
      421 cgggcaatcc acgcatcggg caggacgcag gtgaggtggc aggcatcggc aagatcggca
      481 taccccttgt cggtgagatg ggctctgtgg tgggtgacgg acaggtggtc atcgacttta
      541 ccgctccgca ggccgctatg acccatctgg ccatctccgc ccaggcgaag gtgcctgccg
      601 tggttggaac gacgggattt ggcgccgccg atctgaacag gatcaaggat ctcagctcgg
      661 ctgctccgtg cgttctctcg cccaatatga gcatcggggt caacgtcctg ctgaaggtcc
      721 tcgctcaggt ggcccgtacc ctcggagatg agtatgacgt cgagatcatt gaaacccacc
      781 accggttcaa gaaggacgcc cccagcggga ccgcgttgaa aatggcgcag acgatcgcgg
      841 aggcgttggg ccgagatctg gacaagactg ggatctatgg ccgcaaggga ctggtcggag
      901 agcgcggcaa agaggagatc gcgattcact cgctgcgggc cggtgacgta atcggggacc
      961 atacggtgat cttcgggggc atgggggagc ggattgagat gacgcaccgg gctcacacca
     1021 gggataattt tgctcgtggt gcattgtgcg cagcccgttg gatccttggc caaccacccg
     1081 gcctgtacga tatgcatgat gtgctggggc tgaggtagat agactaaaag acggtagcga
     1141 aggcctgttc taatacttca gcctaaactc cttattgaag gcttatacta tgcggattgt
     1201 gcgattcgtc gccggcaagc aaccaaggta cggtgtggta gagggcaccg tcgtccggga
     1261 gatcgaaggc gacattttcg gcaagtttac cgtgacggac cggacgcatt cgctgaagcg
     1321 gatcaggttc cttgcgccaa ccgaaccctc caagatcgtc ggggtaggtc tgaactatcg
     1381 cgatcatgcc gaggagatga agcttcaaac cccagacgaa ccgattatct tcttaaagcc
     1441 gccgagcgcg acgctccccc atcgtggccg gattacctat ccgaaattgt gccgccggct
     1501 ggattatgag gcggagctgg ggatcgtcat caagcggacc gcgaggaacg taccggtcga
     1561 gaaggctcat cgctacatct tggggtatgt ctgctttaac gatgtgacag ccagagacct
     1621 acagcacatg gacggacaat ggacccggtc aaaatcgttc gatacctttg ccccgttcgg
     1681 tccctgggtc gagacggatc tagacctctc tgatgcccca atccgggcgc tcctgaacgg
     1741 tgtgatccga caggagtcaa acatcaataa cctgatcttt gaggtaccat acctgattca
     1801 ttttatctct acggtgatga ccctctatcc gggtgatgtc attgccaccg gcaccccctc
     1861 tggggtcagt cctatgcggc ccggtgatac gatcgagatc gaggtggaag ggatcggccg
     1921 actgagcaac acgattgttg ctcaaagctg acggccgaaa gccgacagag aatccaggag
     1981 gagaagaaag gcatgggaca accttttagc gtggcacagc ggctgtcaaa gctgccgccg
     2041 tatctctttg ctgagatcga tcgtttgaag caggacgcga tccgtcgcgg aatggatatc
     2101 atcaacctgg gagtcggcga ccctgacctg ccgacaccgt cgcacatcat cgcaaggatg
     2161 caggaggctt cggccgaccc gcggaaccac cagtatcctt catacgaggg gatgctgagc
     2221 ttccggcagg cggtggccga ctggtacagc aaacgattcg gcgtgacgct tgatccgcag
     2281 accgaggtgt tgagccttat cggatcgaaa gaggggatcg gccacatccc cctcgcgttc
     2341 atcgatccgg gtgacgtcgt tctggttccc gatcccggct atccggtcta tcaggcagga
     2401 accgtctttg cggatgggat cccgtacttt atgccgctca cgcgagaacg gtcattcctt
     2461 ccggatcttg aagcgatccc accggaggtt ctgaacaagg ctcggctcat gttcctaaac
     2521 taccccaata acccgacggc tgctgtggcg tccagagcct tttttgctga ggcggtcgcc
     2581 tttgcgcgca ggcaccggct gattctctgt cacgacgcag cctattccga gatggcatac
     2641 gacgggtacc ttccggagag cctgttggcg gtggaggggg cgaaggacgt tgccattgag
     2701 taccattcgc tttccaagac gtacaacatg accggatggc ggatcggatt tgcggtcggt
     2761 tgccgtgagg tgctgtccgg ccttggccgg atcaagacga atctggactc cggggtcttc
     2821 caggcggtgc aggaggctgc catcgcggcg ctgaagggcc cgcaggagtg cgttgaggcg
     2881 atgagggccg tctacaaaga gaggcgcgat gcgcttgtgg atggtctgtc ggcgctcggg
     2941 ttcacggtgg acagacctca agcgaccttc tacgtctgga ttggtgttcc gaaggggcat
     3001 acctctgcct ctctggcctc tgcgctactt tccgagaccg gcattgtcat gaccccaggg
     3061 atagggttcg gccggagcgg tgaagggtac attcgagcgg cgctgacggt ggatgtctcc
     3121 aggattaagg aggcggtcga gcggattgcc gcctcgaatc tcaagcctca gtagtttcag
     3181 aaggggaacc agtgtccttc tctaccctat accctatacc ctgggccatg agcgagcgcg
     3241 cgtatatcgg gatcggatcc aatctgggcg accggattca acgctgccag gagactattg
     3301 gggccatctc ggacattgca ggggttacag cgatacgtac ctcgtcactc tatgagaccg
     3361 cgccggtccc tcccgcttct ggtggctggt ttgtcaacgg agtggtctct gtgcagacgc
     3421 agatcaaacc gggagcgttg ctgctcgagc tgctgcggat cgaacagagt atgggccgcg
     3481 cggcggagcg agcgcgaggc gaagatcgga gcatcgacct cgacctgttg tttgtgggtt
     3541 cacagatcgt ggaacagccg gactttgtcc ttccccaccc gcgactgcat cagcgacgtt
     3601 tcgtcctggt tccgctctgc gaacttgatc cggattttcg ccacccggtc tttggcgtca
     3661 cgatgcggca gttgctcgat cgtctcgacg acccgtcgct cgtccgactg ctggtgccgg
     3721 cggcaaggta tacaggatct ggggagaata gctaggtgac agatcgcgcg tccaagcctc
     3781 gttacatcgt ggtcgaaggc cctatcggcg tcggtaagac gagcctggcc gaattgctgg
     3841 cggagcggct gcaggccaga aagtcgttgg aaggtcctga tgagaaccca tttatcgctc
     3901 aattctacgc cgacatgcgt cggtacgcct ttcaagccca actttatttc ctgctgaaca
     3961 ggttccgcca acaacaggaa cttgcccagt tcgacctctt caaacagtcc ctggtgagcg
     4021 actatctgtt tgccaaggat aagatcttcg cgtacctgac gcttgacgat aacgagttgg
     4081 ccctctacga gcgattgcaa ccgcttcttg agacgcgcgt cgtcaagcct gacctcgttc
     4141 tatatctgca ggccagcaca gatgtcctgg tccagagaat tcagtcgcgg gcgagggttt
     4201 ccgagcggga actgggacag gcctacctgg aggacgtgaa cgcagcctac aatcattttt
     4261 tcttccacta ctcggcgacg ccgctgctgg tcgtcaatac caacgagatt gacttcgtca
     4321 agcacaagga ggattttgaa gacttggtca aacaggtcga gacggtgcgg gcgggcaccc
     4381 aatattatgt atcgcttgga tcccgcaagt agggaccgat cgtaacgact taggtgttta
     4441 gactgaagac tgaaggttct caatgatctc gcaaggcacg aatcgggcca gtcaagatct
     4501 tttcggagtt gtgtatgatc acgcatagct tacccctaaa agccttcagc cttcagccta
     4561 tctatctgag tagttacgac cgatcatgac cagtcagacg gtcaggacgg tcaccctgca
     4621 ggagatgaag cgagaagggc ggaagattac tatgctgacg gcgtatgact acccgttggc
     4681 cctgcttgtg gatcgcgccg ggatcgatct gatcctggtg ggtgactccg gggggatgac
     4741 cgtcctgggg tacgagacca caatcccggt taccatggac gagatgctca tgatgaccag
     4801 ggcggtcacc cgcgctgtca agcggtccat ggtggtcgct gacatgccgt tcatgtcgta
     4861 tgaggctgaa ccggccgagg cggtcagaaa cgccgggcgg ttcgtgaaag agggtctggc
     4921 tcacgccgtc aaggtcgagc gcggttggcc ctcgcttcca tgtgtgaggg cgatcgtgga
     4981 cgcggggatt ccggtcatgg gtcacgtcgg acttacccca caaacggccg tattgcagga
     5041 agggttgaag gttcaaggga gagggctcaa cgatgcccgc cgcatccttg aggatgcgct
     5101 ggcgcttgaa aaggctggag tcttcgcggt cgtgcttgag gcgattccca gtgtactcgc
     5161 cagggtgatc accaggcggt taacggtccc caccatcggg atcggtgccg ggccagattg
     5221 cgatggccag gtgctggtca tacatgatct cctcgggctc ttcgatcgtt ttgtcccgaa
     5281 gttcacgaag cagtacgctg atcttgccaa ggtgattagc gacgcggtga gccgcttccg
     5341 ggaggaggtg atcgaatgcc gatttcctga tgccgcacac acatactcga tcgaccagga
     5401 gaccgagaga gcgctgctgg agctgtgaga ggtgcgaggt gtctggaaga atgtaggggc
     5461 gctgcttgct gcgcccgcac gaagtaaaac ctgtgtgggt gcagggttcg ggggaaccga
     5521 aagggtgagg g
//
LOCUS       DATMXI010000016         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_217934, whole genome shotgun sequence.
ACCESSION   DATMXI010000016 DATMXI010000000
VERSION     DATMXI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217934"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..325)
                     /locus_tag="VN444_00365"
     CDS             complement(8..325)
                     /locus_tag="VN444_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20297.1"
                     /translation="MRLLAIGLLVEGMKVAKPVVHDSGRILLAPGVSMTGRMIERLAN
                     QGVGQVWVEAEGLDEGMLSAETAKRMEWALERRFAHVCHDPLMQQIQAIVRKRIRART
                     GSE"
     gene            complement(332..1030)
                     /locus_tag="VN444_00370"
     CDS             complement(332..1030)
                     /locus_tag="VN444_00370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HWR20298.1"
                     /translation="MDGHPHRYEEPQDLRTLVEAAGRMADGEFRELVTVQAKGEIGRL
                     ASFINQTMRNLQELDPSVGVSSRRLPKMIGHLSEVVQETEQASLRVLDEIDRIAEEQA
                     AMGRDLQQLAVLLNEEWGKDDRRNAALQTVARLREIHGRVQGRALEIMGAMEFQDITA
                     QSIQKSMALITEIHDRLLRLLVLFNIPPDEKDGVDVDDKWKAINEFATASASNAMNQE
                     MADRVLAEFGRNGR"
     gene            complement(1124..1816)
                     /locus_tag="VN444_00375"
     CDS             complement(1124..1816)
                     /locus_tag="VN444_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWR20299.1"
                     /translation="MTGNRHDGGGGLRWLLTYADMITLLLAFFIVLYASSRVDGRKYS
                     DLVSSIRIAFGVPLPPRPIVQAGNGGETLLPFPDVVGMLVQQLSSHVEEEIKTGSVEI
                     ERNEKGLILRFRDTVLFGLGSATLSDDAKQILDKVARPLFNMPYAIEVEGHTDLLPIR
                     SSNFPSNWELSVARATAVIRYLVEAHRFPPARLAARGMADNQPLAPNDPARGNPRNRR
                     VELHLLAGQGPA"
     gene            complement(1885..>2154)
                     /locus_tag="VN444_00380"
     CDS             complement(1885..>2154)
                     /locus_tag="VN444_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWR20300.1"
                     /translation="VMGLVHVLSKLGEGTDSLGRGIAVAFLATFYGIFSANVLFLPMS
                     GNLKVKSEAEVRIREVMMEGVLGIQSGLNPHMLEQKLRSYFASMA"
BASE COUNT          440 a          712 c          548 g          454 t
ORIGIN      
        1 tttgacatca ctcactgccg gtgcgggcgc gaatccgctt tctgacaatc gcctggatct
       61 gttgcatgag cgggtcgtgg cacacgtgag cgaaccgacg ttccagcgcc cactccatcc
      121 gcttcgccgt ctctgccgac aacatgcctt cgtcgagccc ttccgcctcc acccagacct
      181 gtccgacgcc ctggtttgcc agtcgttcga tcatccgccc ggtcatggat actccaggcg
      241 ccagcagaat ccgcccggag tcatggacca ccggcttggc caccttcatg ccctccacca
      301 gcagcccaat tgccagcaaa cgcatagtcg gctatcgccc gttccggcca aactcggcca
      361 gcacacggtc cgccatctcc tgattcatcg cattcgatgc ggacgcggta gcaaattcat
      421 taatcgcctt ccacttgtcg tctacatcta cgccgtcttt ttcatctgga gggatgttga
      481 acagcaccaa caggcgcaag agtcgatcat ggatctcagt aatcagcgcc atgcttttct
      541 gaatgctctg ggcggtaata tcctgaaact ccatcgcgcc catgatctca agcgcgcggc
      601 cttgtacgcg accatgtatc tcccgcagac gcgcgacggt ctggagggcc gcattccgtc
      661 gatcatcctt cccccattcc tcattcaaca ggacggcaag ctgttgcagg tcccgaccca
      721 ttgccgcttg ctcttctgca atccggtcga tctcgtcgag tacgcgaagc gaggcctgct
      781 ctgtctcctg caccacttcc gacaggtggc ctatcatctt gggaagacgt cgagaactta
      841 cccccaccga tgggtccaac tcctgcaggt tccgcatcgt ctgattgatg aaagaggcca
      901 gacgaccgat ctctcccttg gcctggaccg ttaccaactc ccgaaactct ccatcagcca
      961 tccttccggc ggcctcgacc agcgtgcgga gatcctgagg ctcctcgtac ctatgcggat
     1021 gtccatccat ggcgttctcc ttctaagggc agttataggc cgtcagctat cggtagccgg
     1081 cctccgctat aagctcgctc atagcgggtc gctcacggca cgattatgca ggcccttggc
     1141 ctgcgagcag atgcaactcc acccggcgat tccgcggatt accccgcgcc ggatcgttgg
     1201 gcgccagggg ctggttatcg gccatccctc tggccgccag gcgagcggga gggaatcgat
     1261 gggcctctac cagataccgg atcaccgcag tcgcgcgcgc aaccgatagc tcccagttgc
     1321 tggggaagtt ggatgacctg atggggagta gatcggtgtg cccctcaacc tcgatggcat
     1381 acggcatgtt gaataggggt cgggcgacct tatccagaat ctgcttggcg tcgtctgaaa
     1441 gagtcgcgct gcctagaccg aagagaacgg tgtcccgaaa gcgaaggatg agcccctttt
     1501 cattccgctc gatctctacg cttcccgtct taatctcttc ctcgacatgc gagctcaact
     1561 gctgaaccaa catacctacg acgtcgggaa acggcagcag tgtctctccg ccattccctg
     1621 cctgcacaat cggtcgcggc ggcagcggga cgccgaacgc aatccggatc gatgaaacca
     1681 gatcgctata tttccggccg tccactctgg acgaggcata cagaacgata aagaaggcca
     1741 gaaggagggt aatcatgtcg gcataggtca acagccaccg taaccccccg cctccatcat
     1801 gcctattgcc tgtcatgtgt cgccctcaga tgtcagcgat cagcactcgc tcgtaactga
     1861 cggctgacag ctcatagctc cctatcacgc catggacgcg aaatacgatc ggagtttctg
     1921 ctcaagcatg tggggattga gccccgactg aatccccaga actccctcca tcataacctc
     1981 tcggattctg acctccgcct cactcttgac cttgagattc ccggacattg gaagaaagag
     2041 cacattcgcg ctgaagattc cgtagaacgt agcgaggaag gcgacggcga ttccccggcc
     2101 aagactatcc gttccctccc cgagcttact cagaacatgg accaatccca tgac
//
LOCUS       DATMXI010000017         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_192917, whole genome shotgun sequence.
ACCESSION   DATMXI010000017 DATMXI010000000
VERSION     DATMXI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192917"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..497
                     /gene="aroQ"
                     /locus_tag="VN444_00385"
     CDS             27..497
                     /gene="aroQ"
                     /locus_tag="VN444_00385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWR20301.1"
                     /translation="MGGAKRGSGRRVLVLNGPNLNLLGRREPDQYGHTTLKEIEDDLR
                     SYAASRAAEIRFVQSNHEGELIDVIHKAIGWADAMIVNAAGLTHSSVGLRDAIIAAAI
                     PTVEVHLSNIYRREEFRHQSLIADVAAGQITGFGAFSYRLGIEAALHLLDKKGS"
     gene            540..1097
                     /gene="efp"
                     /locus_tag="VN444_00390"
     CDS             540..1097
                     /gene="efp"
                     /locus_tag="VN444_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWR20302.1"
                     /translation="MIAAGDLRSGVKIELDATPYVVTDYQWVKPGKGGAFMRTKLKNM
                     KTGAIIERTFRTDEKLPKAEVEDRKVQFLYHDGDVYHFMDTQSFDQFTMGEKELGDAS
                     GFLKEGMISSVLFYRDEPVDVVLPTFVELRVVETDPGFRGDTASGGSKPATLETNAVI
                     QVPLFINVGDLLRVDTRSGTYVERA"
     gene            1165..1800
                     /gene="thiE"
                     /locus_tag="VN444_00395"
     CDS             1165..1800
                     /gene="thiE"
                     /locus_tag="VN444_00395"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWR20303.1"
                     /translation="MIPWKTCRLCVITDRELRRGLSHVDIAARAVQGGASMIQLRDKV
                     AGPRQLLREARQIAQLCRERGVCFIVNDRLDLALAADADGVHLGQDDLPPRNARALLG
                     TSKFLGVSTHSLEQALRAAEQGADYLGIGPVFATATKVTGYEPVGCDTIRQLRARIDI
                     PIVAIGGIRLSNVGEVIQSGATGVAVISAIGDTDDITAATRAFVTAIEQAE"
     gene            1888..3000
                     /locus_tag="VN444_00400"
     CDS             1888..3000
                     /locus_tag="VN444_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWR20304.1"
                     /translation="MGRRWLWPWKALVLILLVSANAHAASTIKVGVAAPLTGDQATLG
                     QELKGGAIIAVEEWNAKGGLLGRKIEIVWGDDQHDPRQAVAVANKFVNEGVVGVIGHF
                     NSSCSIPASTIYRDGKVVQLTPASTNPQFTERGLWNVFRVCGRDDQQGGVAANFIVRK
                     LQKRRIAVLHDKTTYGQGLADETVKALEKARIKPVYYGGVTQGEKDYRPVLTAVRPSD
                     PEVLFYGGIYPEAILLTKQMRELGMKTVFMSGDGVWAKAFIDIAGKAAEGSFITFTPD
                     QTKIKEAQEVIKRHKEKFGTEVGAYTVYSYVAATVLFEAIAAIQSTDSIKIADHIRKT
                     KWKTALGPIQFDKKGDVLVSPYVIWEVKDGKFVELQ"
     gene            3257..>3408
                     /locus_tag="VN444_00405"
     CDS             3257..>3408
                     /locus_tag="VN444_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWR20305.1"
                     /translation="MLLQQLVNGLTLGSVYALIALGYTMVYGIIELINFAHGEIYMLG
                     AYMGIIT"
BASE COUNT          776 a          914 c         1025 g          693 t
ORIGIN      
        1 catccgtgca gcgaggagtc tgtgagatgg gtggcgcgaa acgcggatcg ggaagacgag
       61 tcctggtgtt gaacggcccc aacttgaacc tgttgggccg tcgcgaaccg gaccagtatg
      121 gtcatacaac gctgaaagag atcgaggatg atctcaggtc ttatgccgcc tcgcgagcag
      181 ccgagatccg cttcgttcaa tcaaaccacg aaggtgaact tatcgatgtg atccataagg
      241 ctatcggatg ggctgatgcg atgattgtga acgcagccgg cttgactcat tcgagtgtgg
      301 gactgcggga tgcgatcatt gccgcagcca tcccgacagt cgaggtgcat ttgtcgaaca
      361 tctaccgccg cgaagagttt cgacatcaat ctctcattgc cgacgttgcc gccggacaaa
      421 tcacaggctt cggcgccttt agctatcggc ttggcataga ggccgctctt cacctgcttg
      481 ataaaaaggg atcgtgagat gaaggctcag gtctcgctca ctcatggaga aaagagatga
      541 tgattgctgc gggagatctt cgctccgggg tcaagatcga actggacgca accccatacg
      601 tcgtgaccga ctatcagtgg gtcaagcctg gcaaaggcgg cgctttcatg cgcaccaagc
      661 tgaagaatat gaagaccggc gctatcatcg agcgcacctt caggaccgat gagaagcttc
      721 cgaaggcgga ggtggaggat cggaaggtgc aattcctcta tcatgacggc gatgtctacc
      781 acttcatgga cacgcagagt ttcgaccagt tcacgatggg cgagaaagag ctcggagatg
      841 ccagtggctt cctgaaggaa gggatgatca gttcagtcct gttctatcgt gacgaacctg
      901 tcgatgttgt gctcccgacc tttgtcgagc ttagggtggt cgagaccgat cccggctttc
      961 gtggcgacac agcctccggc ggctccaagc cggcgacgct cgagaccaac gccgtgatcc
     1021 aggtgccgct gttcattaac gtcggtgacc tcctccgcgt cgatacacgt tccggtacct
     1081 atgtggagcg agcctgagga ccggccaatg agctgacagc tgagagctaa tagctgacag
     1141 cttatttctc accgagatag ctccttgatc ccttggaaga catgccgctt gtgtgtcatc
     1201 accgatcgtg agctgagacg cgggctgagc cacgttgaca ttgcggcgcg agccgtgcag
     1261 ggcggggcat cgatgatcca actccgggac aaggtggccg gacctcgtca gcttttgcgt
     1321 gaggcacgcc agatcgccca actgtgtcgg gagcgtgggg tctgcttcat tgtcaacgac
     1381 aggctggatc tcgcgctcgc ggctgatgcc gacggggtcc accttggaca ggatgacctg
     1441 ccgcccagga acgctcgcgc gctcctgggt acgagcaagt tcctgggggt ttcgacccac
     1501 tccctggagc aggccctcag ggccgcagag cagggggctg attatctcgg catcgggccg
     1561 gtcttcgcga cagccacaaa agtcaccggg tacgaaccgg ttggctgcga cacaattcga
     1621 caactacggg ccaggatcga tatcccgatt gtagccatcg gcggcatcag attgagcaac
     1681 gtcggagagg tcattcagag cggagctact ggagttgcgg tgatctcggc gattggcgac
     1741 actgatgaca tcacggcagc cacgcgggcc ttcgtgacag ccattgagca ggcagaataa
     1801 ggccgctgat agcgtgcagc gtctattgcg tctatcgcta tgacgcaatg aacgcaatga
     1861 cgcaatgacc caacagagga gacgaggatg ggacggcggt ggttgtggcc ttggaaggct
     1921 ctcgtactga tcctgctcgt ctccgcgaac gcccatgcgg ccagcactat aaaagttggg
     1981 gtggctgctc cgctgacagg cgatcaggcg accttgggac aggagttgaa gggcggcgcc
     2041 atcatcgccg tggaggagtg gaatgccaag ggaggcctcc tcggacggaa gatcgagatc
     2101 gtatggggtg acgaccaaca cgatccaagg caggccgtcg cagttgccaa caagttcgtg
     2161 aatgaagggg tcgtcggagt gatcggccac ttcaacagca gttgttcgat tccagcttcc
     2221 accatctatc gcgacggcaa ggttgtccag cttacccctg cctccaccaa cccgcagttc
     2281 accgagcgcg gcctgtggaa cgtcttccgc gtgtgcggca gagacgacca gcagggtggt
     2341 gtggcggcca acttcattgt gaggaagctg cagaagagaa ggatcgcggt cctgcacgac
     2401 aagaccacat acggacaggg gctggctgat gaaaccgtga aggcccttga aaaggccagg
     2461 attaagcctg tctactacgg gggcgttacg cagggagaaa aagactaccg accggtgctc
     2521 accgcagtga gaccgagcga tcctgaagtc ctcttttatg gcggcatcta tcctgaggcc
     2581 attctgctga caaagcaaat gcgtgagttg ggaatgaaga cggtctttat gagcggcgac
     2641 ggcgtctggg caaaagcgtt catcgacatt gccggcaagg cagcggaggg ctccttcatc
     2701 acctttaccc cagaccaaac gaagatcaag gaagcccagg aagtcatcaa gcggcacaaa
     2761 gaaaagttcg gtactgaggt tggagcctac accgtgtata gctatgtggc tgccacggta
     2821 ctctttgaag cgatcgccgc gatccagtct accgatagca tcaagatcgc ggaccatatc
     2881 aggaagacca agtggaagac cgccctcgga ccgatccagt tcgacaagaa gggcgatgtc
     2941 cttgtgtcgc cctacgtgat ctgggaggtg aaggacggga agttcgtgga actccagtag
     3001 gaggtaacta cttaggcgtt taggctgaag gctctacatt ctctcgcacg gcacgaatca
     3061 agccattcaa gaccttttcg gtttccacga cctgtggtct attagagagg actcctcatt
     3121 gagcaagaag cccacacgct tcgacaaatt gcgacgtcat cagccaactc aaatgcccga
     3181 agatttgtgt gatcactgct ttccccctaa cagccttcag ccttcagtct atctacctaa
     3241 gtagttacga gcggagatgc tgcttcaaca gttggtgaac gggctgacgc tcggtagtgt
     3301 ctatgcactg atcgcgttag gctataccat ggtctacggg atcattgagt tgatcaactt
     3361 tgcccacggt gaaatctaca tgttgggcgc ctacatgggg atcatcac
//
LOCUS       DATMXI010000018         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_170901, whole genome shotgun sequence.
ACCESSION   DATMXI010000018 DATMXI010000000
VERSION     DATMXI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170901"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..2366
                     /locus_tag="VN444_00410"
     CDS             540..2366
                     /locus_tag="VN444_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HWR20306.1"
                     /translation="MTTGPTPTGNTVPLRISGFSTLAEALDYAAQGETGCNFYGDQGE
                     LHAVLPYADLREQARSLARRLLSLGLDRGSRVAIVADTDPDFQRFFFACQYAGLVPVP
                     LSASLLISGHKRYVAQLRALLENCQPSVAVAGPEFFSFLSEAAEGLELDHIGTVEAFT
                     CLPEVQRELEPLGSTEMAYIQYTSGSTLLPRGVMITQTAVLANLEGILARGLQIRPGD
                     RAVSWLPFYHDMGLVGFVLAPMVGQVSVDYLKTRSFAMRPRLWLSLMTQTRATISFSP
                     TFGYEMCARRLRRDDVGKFDLSAWRAAGVGAETIRPDVLEEFATAFAPSGFKPGAFLA
                     CYGMAEASLAISFASLGEGITVDRVEQTPLSERGVAVPLNGRGCETETDGTNVSRFVN
                     CGTALPGIQVEVRDEQGQVLPDRRAGVIFARGANIMSGYLGDRGKTRKVLSSDGWLDT
                     GDIGYLVEGSIVIIGRKKDVIIIHGRNIWPQDLEHLAEELPEIRIGDACAFSIETPNR
                     TELAVLVVQYRELNAVKRTECIHRLQGLIREQFAIDCFVELVPPRTLPRTSSGKLSRS
                     KTREEFLNRNNVAQLFHSRNGSAETRPQQPRRQADPCGTLLR"
     gene            2345..3298
                     /locus_tag="VN444_00415"
     CDS             2345..3298
                     /locus_tag="VN444_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWR20307.1"
                     /translation="MRDVVALTGATGFIGSAVARRLIQGGWRVRALRRSPSLTARLSG
                     TSLQWVLGTFEDRDCLEDLVGDADAVIHCAGAVRGITEADFYGANVEAVSRLAHIAAS
                     RSPVPRFLLISSLAAREPQLSPYAASKRMGELALSAVADRLPWTALRPPVVYGPEDRA
                     MRPLLRLMLYGIAPILGREDARFSLLYVEDLAEAVIQWLIGNVREKRAFELDDGHLNG
                     YTWHEVIETFQRLRGKRVIHFQVPWLVPNIVARLNKMVASVIGYAPMFTPGKVREFRH
                     PDWVCDNAPFCRVANWTPKVRLEEGLSLTLDFPLPGQGTGA"
     gene            3326..3574
                     /locus_tag="VN444_00420"
     CDS             3326..3574
                     /locus_tag="VN444_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWR20308.1"
                     /translation="MAHYETILPKLYEILQPHGQEGQTLSEETELVADLNLDSLKVME
                     LLLELEERFDISIPLNIVSDVRTIGDFARQIEQLMEHK"
     gene            3574..>3802
                     /locus_tag="VN444_00425"
     CDS             3574..>3802
                     /locus_tag="VN444_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWR20309.1"
                     /translation="MALLKKFRQLADARKSLAMMSEDPFRVTIERLLSPTEAVVNGRP
                     VILAGTNNYLGLTFDPQCIEAAAHAVREQGTG"
BASE COUNT          762 a         1013 c         1164 g          863 t
ORIGIN      
        1 gagagtccgg tttgcctatc aaaggtactt aagtgtagct tagaaacatg ccctgcgcca
       61 gcacaaagga actcttttca agacagacag agtgaccggt gtgggagttg ggggttctga
      121 tccaatgccc ctgcacggta ggagggggtt gctgggctaa tcaatgatta tgatccaccc
      181 tcactttctt gttgtgcaaa acatcaggtc ttgtatataa tgcgaagtca tcagttaggt
      241 ggtaagatgg gctggttcat tcatggcttt gagtgcccat tattatccac ggcgtcatga
      301 caactttcga gatgtatcca tttgccgcca gcgtgagtct tgtatgaggg cgctgccttc
      361 cgtctcatgc ttggcgcgat gcgtgtggcg cgtcgcctcg tggtcttgcc ccatttagga
      421 gtaggggaac agttcggtcg gtcctactcc gtcttggctt gctggtgagg gatgccgctc
      481 atgttaagag gcaggaatga tcaggcaacg gcgtaatgca cagagggggt aggggaagga
      541 tgacaacggg accgacgccg actgggaaca ccgtgccgct tcgaatcagt ggcttttcta
      601 cgttggccga ggctctggat tatgcagccc agggagagac tggatgcaat ttctatgggg
      661 accagggcga gctgcacgca gtcctgcctt atgctgatct tcgagagcag gcacgatcac
      721 tggcgcgccg ccttttgagc ctgggactcg atcgaggatc tcgagtagcg atcgttgcgg
      781 acacagatcc ggattttcaa cgcttctttt tcgcgtgtca gtatgccggc ctcgtacctg
      841 tccccctgtc ggcttcgctc ctgataagcg gtcataaaag gtatgttgcc caactcaggg
      901 cgctcttgga gaactgccag ccgtccgtcg cggtggctgg gcctgagttt ttctcatttc
      961 tgagtgaggc cgccgagggt ctcgagttgg atcatatcgg gactgttgaa gccttcacgt
     1021 gccttccaga agtccaacgg gagttagagc ctttaggctc tacggagatg gcctacatcc
     1081 aatatacatc ggggagcact ctgttgcccc gcggggtgat gatcacccaa actgcggtac
     1141 tcgccaatct ggagggcatc ctcgcgcgcg gtcttcagat tcgtcccgga gatcgcgctg
     1201 tatcctggct acctttttat catgacatgg ggcttgtcgg gttcgtgctc gcgccgatgg
     1261 tgggccaggt ttctgtggat tacctgaaaa cgcgatcctt tgccatgcga cctcgattgt
     1321 ggttgtccct gatgacgcag accagggcca ccatctcctt tagtccgacg ttcgggtacg
     1381 agatgtgtgc gaggcgtctc agacgggatg atgtcggcaa gttcgatctc agcgcgtggc
     1441 gagccgcagg agtaggcgct gaaacgattc ggcctgatgt cctggaggag tttgccacag
     1501 ccttcgcgcc cagcgggttc aagccaggcg catttttggc ctgctatggt atggcagagg
     1561 cgtccctcgc catcagcttt gcatccctcg gtgaagggat cactgttgat cgcgttgagc
     1621 agacgccttt atctgagcgt ggagtggcgg tgccgctcaa tggacgtggg tgcgaaacgg
     1681 aaacggatgg gacgaatgtg agccgatttg tcaattgcgg caccgctctc cctgggatcc
     1741 aggttgaggt gcgagatgag cagggtcagg ttctcccgga ccgccgcgcc ggcgtgatct
     1801 ttgcgcgggg cgccaacatc atgtccggat acctcggaga ccgtggaaaa actcggaagg
     1861 ttttgtcctc ggatgggtgg cttgacaccg gagacattgg ctatctcgtg gaagggagca
     1921 ttgttatcat tggccggaag aaggatgtca ttatcattca cggtcgaaat atctggcctc
     1981 aggacttaga gcatctcgcc gaggaattac ccgaaatcag aataggcgac gcgtgcgcgt
     2041 tttcgatcga gactcccaac cggactgaat tggccgtact ggtcgtccag taccgggagc
     2101 tgaatgccgt caagcggacc gaatgtatcc atcggttgca gggcttgatc cgcgagcagt
     2161 tcgcgatcga ctgtttcgtt gagctcgttc ctccccgtac gctgccgcgc acgtcatccg
     2221 ggaaactttc ccgctcaaag acccgggaag aattcctgaa ccgcaataac gtagcccagc
     2281 tctttcacag ccgaaacgga tccgccgaga ctcgtccaca gcaaccccgc cggcaagcgg
     2341 acccatgcgg gacgttgttg cgttaacggg ggcgacggga tttattggga gcgctgtagc
     2401 ccggagactg atacaaggag gatggcgcgt acgcgcgctg cgccgttcac cgtctctgac
     2461 agcccgcctt tcgggaactt cgcttcagtg ggttctgggc acattcgaag atcgcgattg
     2521 tctcgaagac ctggttggcg atgcggatgc tgtgatccat tgcgcggggg cggtgcgcgg
     2581 gatcaccgaa gccgactttt acggagcgaa cgttgaggcg gtctcacgac tggcccatat
     2641 cgctgccagc cgaagccccg tgcccagatt tctgctgata tcatcgttgg cggctcgaga
     2701 gccgcagttg tcaccctacg cagccagcaa gcggatggga gagcttgccc tatctgcagt
     2761 ggccgatcgg ctcccgtgga ccgcgcttcg ccctccagtc gtctatggcc ccgaggaccg
     2821 cgcgatgcgt ccactgcttc gcctgatgct gtacgggatc gcccccattc tgggtcgaga
     2881 agacgccagg ttctccttgc tctacgtgga agacctggca gaagccgtga tacaatggct
     2941 gatcggcaat gtgcgagaga aacgagcgtt cgagttggat gatgggcatc tcaacgggta
     3001 tacctggcat gaggttatcg agaccttcca gcgacttcgt ggaaaacggg tgatccattt
     3061 ccaggtgcca tggctggtcc caaatatcgt cgcgaggctc aacaagatgg tcgcatccgt
     3121 gataggatat gcgccgatgt tcaccccggg aaaggtgcga gagttcaggc acccggactg
     3181 ggtctgcgat aacgcgccgt tttgccgggt tgccaactgg acgccaaagg tgcgtttgga
     3241 agaaggactg agtctcactc ttgattttcc cttgccaggg caagggacgg gcgcataacc
     3301 tcattcacgt caggctgact tacagatggc tcactacgag acgatactgc cgaaactcta
     3361 cgagattctg cagccgcatg ggcaggaggg acagactctt tcagaggaga cagagctcgt
     3421 agccgatctc aatctcgatt cgctgaaggt catggagctt ctgcttgagt tggaggaacg
     3481 gttcgacatc tccattcctc tcaacatcgt ttcggatgtg cgaaccatcg gggatttcgc
     3541 caggcagatc gagcaactta tggagcataa gtgatggccc tcctgaagaa gttccgacag
     3601 ttggccgatg cccggaaatc attggcaatg atgagcgagg atcccttccg ggtgacgatc
     3661 gagcgcctct tatcgccgac cgaggctgtc gtaaacggtc gtccggtaat cctggcaggg
     3721 acgaacaact acctaggatt gacgttcgat cctcagtgta ttgaagccgc ggcccacgcc
     3781 gtgcgggagc aaggaaccgg ta
//
LOCUS       DATMXI010000019         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_304139, whole genome shotgun sequence.
ACCESSION   DATMXI010000019 DATMXI010000000
VERSION     DATMXI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304139"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VN444_00430"
     CDS             <1..630
                     /locus_tag="VN444_00430"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HWR20310.1"
                     /translation="ITEVDLVLVNDESVDLRHVIKENDRISVYPVFESFDISSVTRVR
                     DQPLRQSRFVLDCHLGKLAYYLRMLGFDTLYRPDFHDHELISISNNEQRILLSRDRKM
                     IEESSITRGYRVRETDPKLQAVEILRRFDLFHVARPLQRCLRCNDVLQPIAKEAVLAR
                     LPPMIRERYEEFQACPKCNRVYWKGSHYQWMQAFIAKLLAEDPSPNRGN"
     gene            878..1549
                     /locus_tag="VN444_00435"
     CDS             878..1549
                     /locus_tag="VN444_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR20311.1"
                     /translation="MKKIRALLIEDNRLLREGLTAMLKEQPDIRVVASAGNSDAMQKW
                     RNLKPDVVLLDVGLRSQNSLRLVKSVKKSTPEAKVIVMDLAPVQTELMEYVRAGVSGF
                     VLKDATFDDFLKTIRSVANGAKVLPPVLTGSLFSQIVDHATQKGKGNPFKSVKMTRRE
                     REVIELISEGLSNKEIAQRLSCAIDTVKTHVHNILEKLALHTRLEIASFAHADDGFEP
                     LSRNQ"
     gene            2403..2588
                     /locus_tag="VN444_00440"
     CDS             2403..2588
                     /locus_tag="VN444_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20312.1"
                     /translation="MQCPLQEITMHLPSLKKAVAATNEPYNQHVREQTLEQEHREDAW
                     KKERKAVDNIAETLQFD"
     gene            2680..2826
                     /locus_tag="VN444_00445"
     CDS             2680..2826
                     /locus_tag="VN444_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20313.1"
                     /translation="MNEATRDDAVSIARLLDVNKETVWKIIKGMKYSTDSELAMAVHS
                     AFVL"
BASE COUNT          773 a          757 c          761 g          731 t
ORIGIN      
        1 atcaccgaag tcgatctcgt tctcgtgaac gatgagtctg tcgatctgcg acatgtcatc
       61 aaggagaatg accggatcag tgtatatccg gtctttgagt cgtttgatat atcgtcggtg
      121 acgagggtgc gtgatcaacc gctccggcag tcacgcttcg tgctggactg tcatcttgga
      181 aaactcgcct actatctcag gatgcttgga ttcgacactc tttaccgtcc cgactttcat
      241 gaccacgaac ttatttccat ctccaacaat gagcagcgta tcctgcttag tcgggaccgg
      301 aaaatgattg aggaaagttc gatcacgagg gggtaccgtg tacgtgagac cgatccaaag
      361 cttcaggccg tggagattct caggcggttt gatctcttcc atgtggccag gccgctgcaa
      421 cgctgcctgc ggtgcaacga tgttctccag ccgattgcca aagaggccgt gctggctcgt
      481 cttcccccca tgatcaggga acgatatgag gagtttcagg cttgcccgaa gtgcaaccgc
      541 gtatactgga aaggctccca ttatcaatgg atgcaggcgt ttatcgccaa actcttggcc
      601 gaagacccgt ctcccaaccg gggaaattag ggatgagctg cctttgtttg ttgtctattc
      661 ggcattctgt tgtcaaccta tcgggtcttg gcggagtcta attactgacc agtactcctt
      721 accttcccct tgaaagccgg ttcaggactg accatcttgc cggctttcgc ctttcttggc
      781 ctaatcagag cttgaaatac acatgccaat ttcttagttt atatcaagtc cactagctag
      841 tgtcctggtc aaggtacccg caaggttctt ccagccaatg aaaaagatcc gtgccctgct
      901 tattgaggat aatcgccttt tgcgcgaagg cctcacggct atgctcaaag agcaaccgga
      961 tatcagggtg gtggcgtcag ctggaaacag cgatgccatg cagaaatgga ggaacttaaa
     1021 acctgatgtc gttctccttg acgttggact gcgaagtcag aatagtttgc gcttggttaa
     1081 atccgtcaag aagagcacac cggaagcaaa ggtgattgtg atggatctcg cccccgtgca
     1141 gaccgaactc atggagtacg tgcgggcagg tgtttccggg tttgtcctca aggatgccac
     1201 gttcgatgac ttcctcaaga caattcgctc cgttgccaat ggagccaaag tcctgccgcc
     1261 agtgttaacc ggatccctgt tttcccaaat tgttgatcat gcaacacaga agggaaaagg
     1321 taatccgttc aagtctgtga agatgactcg tcgtgaacgt gaagttattg aattgatctc
     1381 agaggggttg agcaacaagg aaatcgcaca gcggctcagt tgtgcaatcg atacggttaa
     1441 aactcacgtg cacaacatcc tcgaaaagct tgcgctgcac actcggctgg aaatagccag
     1501 cttcgcgcac gccgacgatg gattcgaacc actatctcgg aatcaataag tcctgagtcg
     1561 agagtgatca ggtctcctgc cgaaaccgac tcaacaccac attccgggtg caagtataaa
     1621 taaactaaat gtcttgctat tgacgcggga agcacccaca ttgatacacc aatgtttggc
     1681 ccaccggtac tcgccactca agggcgagac tctctttggc tcgcataagg tttgcccgta
     1741 aacagcgttc gttgttagcc cacttcgatc cggtatgtaa cgctgcttct ccggtactct
     1801 cggtacggta acacaccaat tcttctcacg ttacatcgcc gttgagatgc atccagaaga
     1861 atgcagttga taggagatat tccgtatgaa aaccattcat catcaggcaa aacaccacag
     1921 gggaagttcg ccccatcagg aaaaggatcg agcgggaggc gagcgcctga gacgacttct
     1981 gtccgggacg cagtttctca aaggtacctt ggttggtctc gacgggtcaa tcctccgaag
     2041 acctgttaga cagatggaaa cctctcggat ggagataacg actcaggaat ctcatccgct
     2101 atggcaatgg gacgtggcag ggcgttggat ctgaactcaa gaggggaaga ttgttcactt
     2161 caaacaaagg aactctcatg tctcaaacca attacatctc gtccacagag cccaaggaag
     2221 atatcataat tgttggcctg gacagaaaca aaacccgcag ggttaccggg tcatacactg
     2281 gatatcatgt gtaccttgaa ttttcaagga ctccgccaca ggcatggagg gaaatttgcg
     2341 ttcgggaatg gaacaatttg aaccagagcc tcggagtgaa aatagatgga agatatcttg
     2401 tgatgcaatg tccacttcaa gaaatcacga tgcatttgcc gtctcttaag aaggccgtcg
     2461 ccgcaacgaa tgagccgtac aatcagcatg ttcgagagca aacattggag caggagcatc
     2521 gagaggatgc gtggaagaaa gaacggaaag ccgttgacaa tatcgccgag acgttgcagt
     2581 tcgattgatg tgtaagttgt gtaagattcc tcaagtcacc cgtttggaca ccagactctg
     2641 cccgagtgcg caacctgtca tgtatttata gaaagactca tgaacgaagc aacccgtgac
     2701 gacgcagtct ccattgcccg gcttctcgac gtcaataagg agacggtttg gaagatcatc
     2761 aaaggaatga agtactccac tgattctgag ttggccatgg cggttcacag tgctttcgtc
     2821 ttgtaacagg ctcatctcct ttgcgggagg gggccatctg actctcatgt ttctctgacg
     2881 aagttcacgg catcggatcg aattcgaacc ctcaccccaa gggactagtc cccttttcaa
     2941 cctttcgagc tatttctgtt tgcattggcg cttggtagct taaaacatgc aagctccgat
     3001 caaaaaccgc agacgaacat ag
//
LOCUS       DATMXI010000020         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_304124, whole genome shotgun sequence.
ACCESSION   DATMXI010000020 DATMXI010000000
VERSION     DATMXI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304124"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VN444_00450"
     CDS             complement(<1..553)
                     /locus_tag="VN444_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009438038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWR20314.1"
                     /translation="MSEQGNASNFIRDIVAADHAAGKHGGRVVMRFPPEPNGYLHIGH
                     AKSICLNFGIVNNIPGGVCHLRFDDTNPTTEDPEYVQAIQNDITWLGFDWHGKMFFAS
                     DYFERLYEFALRLIQKGLAYVDSLTAEEMRAYRGTLTQPGVNSPSRSRSIDENLDLFR
                     RMRAGEFADGTHVLRAKIDMASPN"
     gene            1538..>2370
                     /locus_tag="VN444_00455"
     CDS             1538..>2370
                     /locus_tag="VN444_00455"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HWR20315.1"
                     /translation="MEHASQPALNEAEREELRALVSQAVEAIPPNWPLRTFAYRNPLI
                     GFEHLPFDEAISQAKQVLGGEGYLSTAEYRACYAEGRISEKELLSALRSRMPEFASQD
                     RVRAGERSLHPEEILLFHFVYGIDPLDPKLLPWQLTEEDATHRIRPDVPQPIKDRLRS
                     HAAGRDPEAVYIHSLWSAAFLKALDLSEDGSTGNHGHSLATAKAGGSVHAEHGPAQQR
                     IRTAAEIIDDLTGSDLKREINEHMIKWCAAFVDEGMADWPMPSRRHGFYRAWRDLASR
                     EA"
BASE COUNT          529 a          633 c          666 g          542 t
ORIGIN      
        1 tattgggtga tgccatgtcg atcttggcgc gaagaacgtg ggttccgtcg gcaaactctc
       61 cagcgcgcat gcgtcgaaag aggtcaaggt tttcatcgat cgaacggctg cgagaggggc
      121 tgttaactcc cggttgggtc agggtgcctc gataggcgcg catctcctcg gcggtcaatg
      181 agtcgacgta tgcaaggcct ttctgaatca atcgtaaagc aaactcgtag agtcgttcga
      241 aataatccga ggcaaaaaac atcttcccgt gccagtcgaa tcccagccac gtgatgtcgt
      301 tctgaatggc ctggacatac tcaggatctt cggtggtcgg attggtgtcg tcgaatctga
      361 gatggcagac gccgccgggg atattgttca ctatgccgaa attcaggcag atcgatttcg
      421 cgtgtccgat atggagatag ccgtttggct cgggcggaaa gcgcatgacg accctgccgc
      481 catgtttgcc ggctgcgtga tcggccgcca cgatgtcgcg gataaagttt gaagcgttcc
      541 cctgttcaga catgcacggt cattgaccca tagagatgtg tgagaggatc atcgcaggca
      601 gttctaccat aagcaaggca caggggagaa ggcccagcct ttcgccagtt aaatatcctg
      661 gctcactctt gtccaaatga ggtttttaga tggtaaacaa agaactttcg ttatgaatcc
      721 acagtttcac cccctgcggt tgcgacagag gaaaatgcga cgttgacagc taatcatgcg
      781 gctgctataa acgcacggct ttcttgtctc gcaaagagga gacagtacag ggcgcactcg
      841 taactccagc ccaccacata ataacggtaa tgcggcatgg actgtgggta gataaaaccg
      901 acctcgagtc ccttgaaggc ctcgggggta gtttttgctg attgtagcaa aggttttttg
      961 gaatcccgaa ggtccaaagc cgtatgctca cgttgttgtt cctccttctt gccgcgtaca
     1021 caatcgtcat cctgcttcca ctgactgttc cgcgaatgcc gcaagtccaa gggattcttg
     1081 gtactgtctg cgcgggtgtc gcaatcacct attgtcaacg tagtcgaata gggctgattt
     1141 tctacagtgg ccagttcgga ggaatggcac ctggacatct ggtcgtatct catcggcgag
     1201 tcgctggttc ttaggcattc ggctgaatct ctttctgagg cagtttagtc gggttcaggc
     1261 cattccgcca caggagcttg caagaaaaag ccttcgccac tttatgagta cgaccaggta
     1321 acatattgat tgctcaaatg aacttctgtc attgccgaat tatatccggc ggcaccaagc
     1381 ttgctgaaca actgttgaac aatcaatgcg ttatgaattc gtgtatggcc taaatttcgt
     1441 tcaaatttac ataatgtgac aataccagag ccaaggtgtg ccgcttgacg gcggccttgc
     1501 gtttgcggaa tttggcccag ggaagagcga cagacatatg gaacacgcct cccagcctgc
     1561 actgaacgag gcagagcgtg aggagcttcg agccctggtg tcccaggcgg tcgaggccat
     1621 tcctccgaac tggccgcttc gcacgttcgc ctatcgcaat cccttgatcg ggttcgaaca
     1681 tttgcccttt gatgaagcta tctcgcaagc gaagcaagtg cttggaggag aaggctatct
     1741 ctccacagcg gagtaccgag cctgctacgc cgagggacgg atttccgaga aggagctgtt
     1801 gagcgcccta cgatcgcgga tgccggagtt cgcatcgcag gacagggtcc gtgccggtga
     1861 gcggagtctc catcctgaag agatcctcct attccacttt gtgtatggca tcgatccgct
     1921 cgaccccaaa ttattgccat ggcaactcac ggaggaagac gccacccacc gtatccgtcc
     1981 tgatgttccc cagcccatca aagatcggct gagatcgcac gcggcggggc gcgaccctga
     2041 agcggtctac atccattcgc tgtggtccgc ggccttcttg aaggcgctcg atctctctga
     2101 agatggatcg actggcaacc acgggcattc cctcgccacg gccaaggctg gaggatcggt
     2161 gcacgccgag catggaccgg cgcagcagcg catccgcacc gcggccgaga tcatcgacga
     2221 tctcaccggc tctgatctga aacgagagat caacgaacac atgatcaagt ggtgcgccgc
     2281 attcgtggat gaaggcatgg cggattggcc gatgccctca cgcaggcacg gattttatcg
     2341 ggcttggcgg gatctcgcgt cgcgcgaggc
//
LOCUS       DATMXI010000021         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_217461, whole genome shotgun sequence.
ACCESSION   DATMXI010000021 DATMXI010000000
VERSION     DATMXI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217461"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VN444_00460"
     CDS             <1..635
                     /locus_tag="VN444_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HWR20316.1"
                     /translation="QAPGGTPHPSPPADRGAATVLTYLDSFRYPLAGEFAMIADLARV
                     NRIPSDWGLEVGVLAQVYRNCAVGRVCQVDLADTYEHKHQDLSATDPTKGLTRMAVDI
                     TKSILRTLAEEGTVLSDGLLKTLPITYIRTARDMLSRYQNDAYINRLAYDQHQEGQAV
                     EAFARAIQLAIEAFLADPLGVPLIPNWNRVLAAIPDFLTRLREAVDADNA"
     gene            619..1461
                     /gene="mpgP"
                     /locus_tag="VN444_00465"
     CDS             619..1461
                     /gene="mpgP"
                     /locus_tag="VN444_00465"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase"
                     /protein_id="HWR20317.1"
                     /translation="MLITHSPEQSPSLLIFTDLDGTLLDHETYSFDPAQEALKEIERR
                     GIPLVLCTSKTRTEVERARGALNNRHPFIVENGGATFIPMGYFPFAHPYQREVGGYHV
                     IEYGMPYPRLIDMLREVRAESGARIAGFSDLSVEQVAALTGLPAEEARLAKVREYDEP
                     FFVKGPPEELDRVKTLFRKRGCRCTRGGRFHHLTGPNDKGKAVSDLVRLFKGAYGERR
                     TVGIGDSQNDLPMLWAVDVPILVQRIDGKHDPTVRVPNLIHAQGIGPKGWRTAVLDLL
                     VRQD"
     gene            1467..2720
                     /locus_tag="VN444_00470"
     CDS             1467..2720
                     /locus_tag="VN444_00470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20318.1"
                     /translation="MEQFLYEPVLERLHEIRKADILIGIPSYNNAGTIGDVVKTVASG
                     LAIYFPSARSVIVNSDGGSQDGTAQVVEAALEDPSLLLVQHPVPPIHKIITPYHGIPG
                     KGSAFRTIFKIAEALQVQACAVVDSDLRSISPEWVERLIRPVYEEGFDYVAPLYRRHK
                     YDGTITNSIVYPLTRTLYGQQIRQPIGGDFGFSGKLAALYLTKPVWETDVARFGIDIW
                     MTITAAAERYRICQAFLGAKIHEHKDPGQHLAGMLVQVVGSLFSLMEEYQGVWQATQG
                     SNPVPIFGPSREVEVEHVSVNVERMVGAFQQGVRDLLPIWEIILAPETLAELLVIAPL
                     EAEAFRFPMDVWVAVVYDFALAYHHRVLHLEHLLKALTPLYLGRIASFVLETQESGHQ
                     QVEGVIETMAERFEAMKPYLVARWR"
     gene            2725..3432
                     /locus_tag="VN444_00475"
     CDS             2725..3432
                     /locus_tag="VN444_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20319.1"
                     /translation="MNELWQEAILDAVQNTGKRLTLFLPNILAMMTLVVIGLVAGWIV
                     KSLLHRILLAVKFDPLCERWGLSQSLTKAGVRRPCSSLVGRLSFWAVFLVFTFMGIDA
                     LNLPAATRLTAGALDFLPQILTALLLLWAGWLLAIFLAQGALIAAVNAQLRGAHLIAT
                     MIRWGVLIFTAATVLTQLGIAKEMVVAAFSIAFGGVVLALALAFGLGGRDLARALLER
                     RLRREKDQEEKDEISHL"
     gene            3413..>4351
                     /locus_tag="VN444_00480"
     CDS             3413..>4351
                     /locus_tag="VN444_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR20320.1"
                     /translation="MRSRISRLGFGAFFIIVFSILTGCAREGSMTEEEGKARIVFAHF
                     KVPSPEALTGLIETFELQNPGVKVVEEILPTSTDQQHQFYVTALEGGSATFDVFALDV
                     IWIQEFARAGWLLDLTPKLGADRLAGFLPGPVEAATYNDRIHAVPWFANAGILYFRKD
                     LLSKYEFAPPRTFMELARQARIILEGEQDPDLRGFVWQGKQYEGLICVALEFIHGNGG
                     SVLEGHRSALRDKKAVEALQFMHDLIAVNKVSPPLVTSADEETTRHLFGAGRAIFMRN
                     WPYALALYEQEGSQVEGRVGIAPLPSFEGYPSAPTLG"
BASE COUNT          837 a         1240 c         1352 g          922 t
ORIGIN      
        1 gtcaggctcc tggtggtacc cctcatccga gccctccagc ggatcgtgga gcagcaaccg
       61 ttttgaccta cctggacagc ttcagatacc cattagccgg ggaatttgcc atgatcgccg
      121 atctggcccg ggtaaacagg atcccgtcgg attggggcct ggaagttggg gtattggccc
      181 aggtctaccg gaactgtgcc gtgggcaggg tctgtcaggt cgaccttgcc gacacctatg
      241 aacacaagca tcaggatctc tccgcgaccg atccgaccaa gggtctcacg cggatggccg
      301 tcgacatcac aaagagcatc ttgaggacat tggctgaaga ggggaccgtc ctgtccgatg
      361 ggctcttaaa gaccctgccg atcacctata tcagaacagc gcgtgatatg ctgagccgat
      421 accagaatga cgcgtatatc aaccggcttg cgtatgacca acatcaagag ggacaggccg
      481 tagaggcctt cgcgagagcg atccagttgg cgattgaggc gttcctggct gatccgcttg
      541 gtgttccttt gatcccgaac tggaaccgtg tgctggcagc gatccccgat ttcttaaccc
      601 ggcttcggga ggcagtggat gctgataacg catagtccgg agcagagccc ctcccttctc
      661 atctttacag atctggatgg aacgttgctg gaccatgaga cgtacagttt cgatccggcc
      721 caggaagctc tgaaggagat cgagcggagg gggatccccc tcgtcctgtg tacgagcaag
      781 acaaggacgg aagtggagcg cgctcgcggt gccctcaaca accgccaccc gtttatcgtg
      841 gagaacggag gcgctacctt catccccatg gggtattttc ctttcgctca tccatatcag
      901 cgtgaagtcg ggggctacca cgtcatcgag tacggtatgc cctaccccag gctgatcgac
      961 atgttgaggg aggtgagagc agaaagcggg gccaggattg cgggcttttc ggacctgagt
     1021 gtggagcagg tggcagcgct cacggggctc ccagcagagg aggcccgtct cgcgaaggtc
     1081 cgggagtacg atgaaccttt tttcgtcaaa ggtcctcctg aggagctgga ccgcgtgaaa
     1141 acgctgttca gaaagagggg ctgccggtgc acgcggggag gaaggttcca ccacctcaca
     1201 ggacccaatg acaaggggaa agcggtgtct gatctggttc ggctgttcaa gggggcgtac
     1261 ggtgagcgtc gcaccgtcgg gatcggggac agccagaacg atctcccaat gctttgggcg
     1321 gtggacgtcc ctatcctcgt ccaacggatc gatgggaagc acgacccgac ggtcagggtc
     1381 cccaatctca tccatgccca gggtatcggg ccgaagggct ggcgaacggc cgtcctggac
     1441 ctgctcgtcc gccaggactg aagaaggtgg aacagttcct ctatgaaccg gtgttggaaa
     1501 ggctgcacga gatccggaag gccgacatcc tgatcgggat cccgagttac aacaatgccg
     1561 gcacgatcgg ggacgtggtc aagacggttg cctccggact cgccatatac tttccctctg
     1621 ctcgatccgt catcgtcaac tccgatggag gctctcagga tgggactgca caggttgttg
     1681 aggccgctct tgaagacccc tccctcctgc tggttcaaca tcccgtgcct cccatccaca
     1741 agatcatcac gccgtatcat gggattcccg gcaaggggag tgcctttcga acgatcttca
     1801 agattgccga agcgcttcag gtgcaagcct gtgcggtcgt cgactcggac cttcgctcca
     1861 tttcgcccga gtgggtggag cggctgatcc gacccgtcta cgaggagggg ttcgattatg
     1921 tggcgccgct gtaccgtcgg cacaagtacg acgggaccat cacgaacagt atcgtctacc
     1981 ccttgaccag gaccctgtat ggccaacaga ttcgacagcc gatcggtggc gacttcggct
     2041 tttcagggaa gctggccgcc ctgtatctca ccaagccggt atgggagacc gatgtggccc
     2101 gcttcgggat cgatatctgg atgacgatca ccgcggcggc cgagcgatat cggatctgtc
     2161 aggcgttcct cggggccaag attcacgagc acaaggaccc ggggcagcat ctcgccggga
     2221 tgctggtgca ggtggtgggc tccctcttca gtcttatgga ggagtaccag ggcgtgtggc
     2281 aagccacgca aggatcgaat cctgtcccca tcttcggacc gtcacgcgag gtggaggtag
     2341 agcatgtctc ggtgaacgtg gaacggatgg tgggcgcctt tcagcagggc gttcgcgatc
     2401 tattacctat ctgggagatc attctggccc ctgagaccct ggccgaactg ctggtcatcg
     2461 ctccattgga ggcggaggcg ttccggtttc cgatggatgt ctgggtcgct gtggtctacg
     2521 atttcgcgct ggcctaccac catcgggtcc tgcacctgga gcatctgctc aaggccctga
     2581 ccccgctcta tctcggcagg atcgcttctt tcgtcctgga gacgcaagag agcggccatc
     2641 agcaggttga aggggtcata gaaacgatgg ctgaacggtt tgaggcgatg aagccgtacc
     2701 tggtggcgcg ttggaggtaa aacgatgaat gagctatggc aagaggctat actcgatgcc
     2761 gtgcaaaaca ccggtaaacg gctgaccctc tttctgccga acatcctggc catgatgacc
     2821 ctcgtcgtca tcggcctcgt ggcagggtgg atcgtaaaaa gcctcctcca ccggatcctg
     2881 ttggctgtga agttcgatcc gctctgtgag cgatgggggt tgagccagtc cctgactaag
     2941 gcaggggtga ggcgcccctg ctcctccctc gttggccgcc tgagcttctg ggccgtcttc
     3001 cttgtgttta ccttcatggg gatcgacgcc ttgaacctgc cggcggccac tcgccttacc
     3061 gcaggcgccc tggacttctt accccagatc ctgaccgctc ttctcctcct ctgggccgga
     3121 tggctgctcg ccatcttttt ggctcaaggg gccctgattg cggccgtcaa tgcccagctt
     3181 cggggagcgc acctcatcgc caccatgatt cggtggggcg tcctgatctt cactgcggcg
     3241 acggtcctca cccaattagg gatcgcgaaa gagatggtgg tggcggcatt ctctatcgct
     3301 tttggtggcg tcgtgctcgc cttggcgctc gcttttggcc tgggcgggag agacctggcc
     3361 agagcgctgc tcgaacgccg ccttcgccgc gagaaggatc aagaggagaa ggatgagatc
     3421 tcgcatctct aggctgggat ttggagcctt cttcatcatt gtcttctcca tccttaccgg
     3481 gtgcgccagg gaagggtcca tgacggagga agagggcaaa gcgaggatcg tgttcgccca
     3541 tttcaaggta ccctctcctg aagcccttac cgggctgatc gagacgtttg agctgcagaa
     3601 ccctggggtc aaggtcgtcg aggagatcct tcccacctcc acggaccagc agcatcagtt
     3661 ctacgtgaca gccctcgagg gcgggtctgc gacctttgac gtctttgcgc tggacgttat
     3721 ctggatccag gagttcgcca gggcggggtg gctcctggat ctcaccccga aattgggggc
     3781 cgataggtta gcgggatttt tgcctggccc tgttgaggcc gcaacctaca acgatcgaat
     3841 ccatgcggtc ccctggttcg cgaacgcagg aatcctctac ttccgaaagg atctgctctc
     3901 gaaatacgaa ttcgcgcctc cccggacctt catggagctg gcacgacagg cgaggatcat
     3961 tctggaaggg gaacaggatc cggatctcag ggggttcgtc tggcagggga agcagtatga
     4021 ggggttgatc tgtgtcgccc tggagttcat ccacggaaat gggggtagtg ttctggaggg
     4081 gcaccggtcg gcacttcggg ataaaaaggc tgttgaggct ctccagttta tgcatgacct
     4141 catcgctgtc aacaaagtct ctccacccct tgtgacatcg gcggatgagg agaccacccg
     4201 tcaccttttc ggagcgggcc gggcgatttt catgcggaac tggccctatg ctctggcgct
     4261 gtatgagcag gagggctcac aggtagaggg gagggtcggc attgccccgc tcccttcctt
     4321 tgaagggtat ccaagcgctc cgacattggg g
//
LOCUS       DATMXI010000022         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_84985, whole genome shotgun sequence.
ACCESSION   DATMXI010000022 DATMXI010000000
VERSION     DATMXI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84985"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VN444_00485"
     CDS             <1..1645
                     /locus_tag="VN444_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20321.1"
                     /translation="QLHDRIDTPLHQLMDVHIREIDDAMRHISDVGQQVVFITFVTLA
                     VSALGAIAISVPLAHWLSRPIRAIAQGSRRLAEGDLSQRVETTGGGELKEAAQAFNEM
                     AQRLERTAIENAALYGAARRRAERIAAVNRLIKMISASFDIGAVYETFAAELKRFVSY
                     TRMGIVIPEDSGTRFKLFQLVGYRLNAVPVGMVWSNVRGTGIEWVMSHRRPHFEQDLA
                     KARRFVEDGALVKEGIRSTVRLPLIATGQVVGVLFLDDVEPDRYSEHDLELLVPLGEQ
                     LAIAIENARLHGEMERKVQERTRTLRETQAQLVQSGKLAAVGTLAAGVAHELNQPLMI
                     IRGYAQELLGDRRIMDEEVRDDLWRIEAQTTRMTAIINHLRDFSRESKGKREITDLNR
                     VVEDALMFLGQQLKSGNIAITRELHPALPTVRIDPLQIEQVLLNLITNARDAMESTGM
                     GAITIRTDVVSGDRVALSVTDTGPGIPEEIRTRIFDPFFTTKEVGKGTGLGLSICHGI
                     VEEHGGELTMESPAVDGTGARFTMILPQAGPDDTTGDRT"
     gene            1642..>2033
                     /locus_tag="VN444_00490"
     CDS             1642..>2033
                     /locus_tag="VN444_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20322.1"
                     /translation="MTGARILLVDDEPEIRRLLSRYLQRLGYTVREAEDGEAAVAAAT
                     VEIPDVVITDMSMPRLGGLELLEQLRNMDPGLPVIVLTGHGSIENVIAAMRRGAAFDY
                     LLKPLPNLTVLEMAVARACEVRTLRAQAR"
BASE COUNT          402 a          543 c          687 g          401 t
ORIGIN      
        1 gcagctacac gatcgcatcg atacaccgtt gcatcagctt atggacgtcc acatacgcga
       61 aatcgatgac gcgatgcgac acatctccga tgtgggccaa caggtggtat ttatcacatt
      121 cgtcacgctg gctgtcagcg cgttgggggc catcgccatc agcgtcccgt tggcacactg
      181 gctgagccgg cccattcgag ccatcgcgca ggggagtcgg cggctggctg agggcgacct
      241 gtctcaacgg gtggagacga ccggtggagg ggagcttaaa gaggcggctc aggcctttaa
      301 cgagatggcg caacggctgg aacgaacggc catcgagaat gccgcactct acggcgcagc
      361 gcgccggcgc gcggaacgga ttgcggccgt caatcgcctg atcaagatga tcagcgcctc
      421 tttcgatatc ggcgcggttt atgagacatt tgccgcggag ttgaaacggt ttgtctcgta
      481 cacacggatg gggattgtga ttcctgagga ctcaggcaca cgattcaagc tttttcaact
      541 tgttggctac cgattgaatg cggtaccggt cgggatggtc tggtcgaacg tgagagggac
      601 cggcattgag tgggtgatgt cccacagacg gcctcatttt gaacaggatc ttgcgaaggc
      661 ccggcggttt gtcgaagacg gggccctggt gaaagaaggg attcgctcta ctgttcgtct
      721 gcccttgatc gcaacaggac aggtcgtcgg cgtcctcttt ctggatgatg ttgagcccga
      781 tcgttacagt gagcacgatc ttgaactgct cgttccgctt ggggagcaat tggccatcgc
      841 catcgaaaac gcccggctcc acggcgagat ggagcggaag gtgcaagagc gcacccggac
      901 gctccgggag acacaggcgc agcttgtcca gtcgggcaag ttggcggcgg tagggacgct
      961 ggcggccggg gtagcccacg agttgaacca gcccctgatg attatccgcg gctatgccca
     1021 ggaactgctc ggggaccggc ggattatgga cgaggaggtt cgtgacgatc tgtggcggat
     1081 cgaggcgcag acgacccgga tgacggctat tatcaaccac ctccgagact tctcacgcga
     1141 gtccaaggga aagcgagaga tcacagatct gaaccgagtt gtggaggatg cgctgatgtt
     1201 ccttggacaa caactgaaga gcggaaacat cgccattacg cgggagcttc accccgcgct
     1261 accgacggtt aggatcgatc cgttacagat cgagcaggta ctcctgaacc tgatcaccaa
     1321 tgctcgggac gctatggagt cgaccgggat gggggcgatt accatccgga ccgacgtcgt
     1381 gtccggtgat cgggtggcgc tgagcgtgac cgataccggt cccggcatcc ccgaagagat
     1441 ccggaccagg attttcgatc ccttttttac gacgaaggag gtcggcaagg gaacggggct
     1501 cggcctgagc atctgccatg ggattgtcga ggagcatggg ggggagctga cgatggagag
     1561 tccggcggtc gatggaacgg gcgcccgatt taccatgatc ttgccccagg ccggcccgga
     1621 tgatacaaca ggtgaccgga catgacgggc gcgcggattc tgctggttga tgacgagccg
     1681 gagatccgtc gattgctgtc acgttatctc cagcggttgg ggtatactgt gcgggaggct
     1741 gaggatggcg aggcggccgt ggcggcggcg acggtggaga ttccggatgt cgtcatcacc
     1801 gatatgtcta tgcctcgcct gggcgggctg gagttgctcg agcaacttcg aaatatggat
     1861 cccggtcttc cggttatcgt gctgaccggg cacgggtcga tcgagaacgt gattgccgcg
     1921 atgcgacggg gggcggcctt tgattatctg ctcaagccgc tccctaacct taccgtgctg
     1981 gagatggcgg ttgccagggc ctgtgaggtc aggacgctgc gcgcccaggc ccg
//
LOCUS       DATMXI010000023         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_280609, whole genome shotgun sequence.
ACCESSION   DATMXI010000023 DATMXI010000000
VERSION     DATMXI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280609"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VN444_00495"
     CDS             complement(<1..1704)
                     /locus_tag="VN444_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWR20323.1"
                     /translation="MKAKYIKLMGILTIIFLMTSPAFASTYGLQNAERTMGAFSQASA
                     GTYTNPLPIEIPGDGLVESCADPTIIYAQTPDDPYWYMYCTTDPLNDEDKTGDNFNFH
                     LIPILRSDDLVSWEYMGDVFSTRPGWVADDAGLWAPEIQYFNDQYYLYYGASWTDLPG
                     GGSAIGVATSDNPLGPWVDSGGPVVEPHAPGCCPGDKRWVFDPEVVRDEDTGQLYIFY
                     GSYFGGISARELSADGLHSDPATQEQITIANRYEGAEVEYHDGFYYLFVSATDCCRGP
                     LTGYTVFVGRSEDILGPYIDREGHSLLEGRVGGTPVISMNGNRWVGPGHNDVFVDFTG
                     QWWTVYHAIDRNDPYFEGATGFTKRPVLLDAIDWIDGWPTVRGGPWASDNPQHAPAAQ
                     PGTKTQYRTKLVKPDRTGKLIQELSDEFNGTLGNQWSWVREPATGTAVSAETFDFQTQ
                     AADLFEGSNNASVLIEDAPNGNYVVETRVRLDLPAEGCCFNYVQAGLVIYGDDDNFVK
                     LVEVSIWETRQTEFAKEWWPVPDGYPRYGNTVVGAPGDWVYLRIVKQTRAGEEHYTAY
                     TS"
BASE COUNT          429 a          573 c          576 g          465 t
ORIGIN      
        1 gctggtgtag gcggtgtaat gttcttcgcc ggcccgggtc tgtttcacaa tgcgcaggta
       61 gacccagtcg ccgggcgcgc caaccacggt gttgccataa cgcgggtagc catcaggcac
      121 aggccaccac tccttggcga attcggtctg gcgcgtctcc cagatcgaca cttccaccag
      181 cttgacgaag ttatcgtcgt ccccatagat gaccaggcca gcctggacgt agttgaaaca
      241 gcagccctcg gcagggaggt ccagccgcac gcgggtttcc accacatagt tgccgttggg
      301 tgcatcctcg ataaggacgg aggcgttgtt gctgccttca aagagatcgg ctgcctgcgt
      361 ctggaaatcg aacgtttccg ccgagactgc agtgcctgta gccggctcac ggacccagct
      421 ccactgattg cccagtgtgc cgttgaactc gtcggatagt tcctggatca gcttgccggt
      481 acgatcgggc ttgacgagct tcgtacgata ttgagttttt gtgccgggct gggcggcagg
      541 cgcgtgctgg ggattgtcag atgcccaggg gccgccgcgt accgtcggcc aaccatcgat
      601 ccagtcgatg gcatccagca gcacaggtcg tttggtaaag ccggttgcac cttcgaagta
      661 gggatcgttg cggtcgatgg cgtggtagac cgtccaccat tgtccggtga aatccacaaa
      721 gacatcattg tgcccgggtc ctacccagcg gttgccgttc atgctgatga ccggtgtgcc
      781 gccgacacga ccttccagga gcgagtggcc ttcccgatca atatacggac ccagaatgtc
      841 ctccgagcgg ccgacaaaga cggtgtagcc ggtgaggggc ccccggcagc aatctgtggc
      901 ggagacgaac aaatagtaga agccatcgtg gtattcgact tccgcacctt catagcggtt
      961 agcaatcgtg atctgctcct gcgtggccgg gtcagagtgc aagccgtctg cgctcaattc
     1021 acgagcggag atgcctccaa agtagctgcc atagaagatg taaagctgcc cagtgtcctc
     1081 atccctcacc acctcgggat caaagaccca gcgcttatca ccaggacagc aacccggagc
     1141 atgcggctcg acgacgggtc cgccgctatc cacccagggt ccgagtgggt tgtcgctcgt
     1201 ggcgactccg atggcactcc cgcccccggg tagatccgtc cacgaggcac cgtagtaaag
     1261 atagtactgg tcgttgaaat actggatttc cggcgcccat aagcctgcat catctgccac
     1321 ccagcccggt cgggtgctga acacatcgcc catgtattcc caactcacga gatcgtcaga
     1381 ccgcagaatg gggatgaggt ggaagttgaa gttatccccc gtcttatctt cgtcgttcaa
     1441 cggatcggtc gtgcagtaca tgtaccagta cggatcatcc ggcgtttgag cgtatatgat
     1501 ggttgggtcg gcacagcttt ccaccagtcc atcaccgggg atctcaatag gtaacggatt
     1561 tgtgtacgtc cccgcactgg cctgactgaa tgcccccatt gttctttctg cattttgcag
     1621 cccataggtt gaggcgaagg cagggcttgt catgaggaaa atgatggtaa gaatacccat
     1681 caacttgatg tatttggctt tcacaggtta ctctccttgg ctggaatagg tgcgacctgt
     1741 cgtgaaatgc atcaatgatg gaatcgccga caaagcggtc tgtacatatt gtcgttgcgg
     1801 catacctcct ttcctctgct gtgcttgctt cttctcaggg caagcgacct tgattttgga
     1861 taaacggtgt ccaaattcta cgccctgcct cacaaaaaat caaccggttt tcagggtgtt
     1921 aaggttacag tttgcccaga aaatagaccg gattcatgta taatactgcc tgtcctgtat
     1981 gaccactaaa gattcatttt ttctgagaga ggaggtgtgc cggacaaaac tggtggggat
     2041 cac
//
LOCUS       DATMXI010000024         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_192085, whole genome shotgun sequence.
ACCESSION   DATMXI010000024 DATMXI010000000
VERSION     DATMXI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192085"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VN444_00500"
     CDS             <1..629
                     /locus_tag="VN444_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWR20324.1"
                     /translation="YPAERIYRDARVNRLFEGTNEINRLLIPTTLFRRTLQGRLPLFM
                     ATQKLMADLLAYSPSQEEAPKGHLGEQIRLLQLAKKITLMVSGAAAQKYRERLADEQE
                     ILGVLSDLVIELFAMESALLRALKSAEHNGEGTAESQSDMVKVYISDAFARIDLLAKE
                     GLASMEEGDALWTQLSALKKLTRYTPCNSTRLRRAIARRIIEAEDYLA"
     gene            complement(705..1127)
                     /locus_tag="VN444_00505"
     CDS             complement(705..1127)
                     /locus_tag="VN444_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWR20325.1"
                     /translation="MLEITDLPLSLELLVNAVKRSSSGAVATFLGVVREQTRGRQVRY
                     LEYEAYREMAIPKLREIADEIHQKWKVDEIAMIHRIGHLQVGEASVAIAVSAPHRHEA
                     LAACAYAIDRLKEIVPIWKKEVWTDGEEWVGPGTCDHR"
     gene            complement(1120..1374)
                     /locus_tag="VN444_00510"
     CDS             complement(1120..1374)
                     /locus_tag="VN444_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWR20326.1"
                     /translation="MKVRVRCFAAVREIVGDGELVVDLPEGSTLIQLISQLRYQFPRL
                     EALTGSLLFSVNREYASTDKGLTAGDEVALIPPVSGGTDA"
     gene            1777..>2447
                     /locus_tag="VN444_00515"
     CDS             1777..>2447
                     /locus_tag="VN444_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20327.1"
                     /translation="MALKQPGIIVKSPSEAIGMKILAGSNKEKALALAQLIEDLLDEL
                     GYTDFHPKLSSIRLVIDLKASHRSSAHRLHCYGCTIPHEVRLEELQGVHRRYMQARRR
                     DKDLIGIFFSVSGFHPTARNWFSRLDAAARGDYHLFGVDKICVLLRRAKLVASLDHIV
                     HTIEGRVMARLGAGNLVFTVGRFYWIFRIEAKRGAAYAVLDAYGSPVSPRVAMSIKRL
                     DGSLKG"
BASE COUNT          547 a          702 c          650 g          548 t
ORIGIN      
        1 gctatccggc cgagcggatc tatcgggatg ccagggtcaa ccgactcttc gagggaacca
       61 acgagatcaa ccggttgttg attcctacga cgctcttccg gcgaacgctg cagggacgtc
      121 tgccactctt catggcgact cagaaattga tggctgacct gctcgcgtac agcccaagtc
      181 aagaggaggc gcctaagggc caccttggtg agcagatccg actgctccag ctagcgaaga
      241 agatcacgtt gatggtctct ggagctgccg cccagaagta tcgcgagcgg cttgcagatg
      301 agcaggagat cctgggggtg ctgagcgatc tggtgatcga gctgtttgcc atggagagcg
      361 cactgctccg tgcgctaaag tcggcagagc ataacgggga ggggacggca gaatcacaat
      421 cggatatggt caaggtctac ataagtgacg ccttcgcccg gatcgactta ctggccaagg
      481 agggcctagc ctcaatggag gagggcgatg ccctctggac tcagctcagc gccctgaaga
      541 agttgacccg ttacacgccc tgcaacagca cccgcctgcg aagggccatc gccagacgca
      601 tcatcgaggc agaggactat ctggcttaac gacctgaagt gcccaaatgc gaggtccggg
      661 acgcctaggg cagccacgag ggctgcccct acacgtcgta cgtcttaacg atggtcacag
      721 gttccggggc cgacccactc ctcaccatcc gtccagacct ctttcttcca gatcggtaca
      781 atctctttaa gtcggtcgat cgcgtaggcg caggcggcga gcgcctcatg acggtgaggg
      841 gcagacacgg cgatggctac actcgcctcc ccgacctgca gatggccgat ccggtgtatc
      901 atggcgatct cgtccacctt ccatttctgg tggatctcat cggcgatctc gcgcaacttg
      961 ggaatcgcca tctcccggta ggcttcatac tctaagtagc ggacctgtcg acctcgtgtc
     1021 tgctctcgca ccacgccgag aaaggtcgcg acagccccgg aacttgaccg ttttacagca
     1081 ttcacaagca actcaagcga aagcggcaga tcggtgattt caagcatcgg ttcctccact
     1141 tacagggggg attaatgcaa cttcatctcc cgctgtcaac cccttatcag tagaagcgta
     1201 ttctctgttg actgaaaata gcagcgaccc ggtcagcgcc tccaaccgtg ggaactggta
     1261 tcgaagctgg cttatgagct gaatcagcgt gctcccctca ggcagatcca caaccagttc
     1321 gccgtccccg acaatctcac gaacggccgc gaaacatcgt actcgaacct tcatcgcagt
     1381 ctctccacga tccggtcgta ccgctccact aagaaaatct cccctcgccc ctcttttcca
     1441 aagaggggta aagcctccct ttggcaaagg gaggtcagga ggaattcttc gatcacgtta
     1501 cgtgattatt ttgagggagt tatcacgaca cattcttcac aacgcctttg tttacgcatg
     1561 ttacgaaata tccctcatgt attatctaga cattgttact tctatcggtt tctcttacca
     1621 ataccatgat ctttccgagt ttgtcaaccc aagacgcttc taagccagta tttaccaaga
     1681 aagcaacttc tcgcttgacc tttgaggctc cttatgttaa ttaaataaaa aaaggttagt
     1741 cgcgagggac cagatgtgca aggagggtta gcgagagtgg cactaaaaca gcctgggatt
     1801 atagtgaaat cgccttctga ggcgatcggc atgaagatcc tggctggatc gaacaaagag
     1861 aaggcgctcg ccttagcgca gctcatagaa gacctcctcg acgaactcgg ctatactgat
     1921 tttcacccta agctctcctc aatccgtctc gtcatcgatc tgaaggccag ccaccgttcc
     1981 agcgctcatc gtctgcactg ctatggatgc acaatccctc acgaggttcg tctggaggaa
     2041 ctgcagggag tccatagacg ctatatgcag gcgcggcgcc gggacaagga ccttatcggt
     2101 atcttcttct ccgtttccgg cttccaccca accgcccgaa actggttttc acgtcttgat
     2161 gccgcggcga ggggtgacta tcacctgttc ggggttgata agatatgtgt cctgcttcgg
     2221 cgtgcaaagc tcgtcgcttc cctcgatcac atcgtccaca cgatcgaagg ccgggttatg
     2281 gcaaggcttg gggcagggaa cctcgtcttt acggtgggaa ggttctactg gatttttcga
     2341 atcgaggcca aaagaggggc tgcatatgcg gttcttgatg cctatggatc accggtctcc
     2401 cctcgcgtgg ccatgtctat caagcggctt gatggctcct tgaaggg
//
LOCUS       DATMXI010000025         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_280420, whole genome shotgun sequence.
ACCESSION   DATMXI010000025 DATMXI010000000
VERSION     DATMXI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280420"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VN444_00520"
     CDS             complement(<1..442)
                     /locus_tag="VN444_00520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWR20328.1"
                     /translation="MNIPNSLTILRILLIPCYIGLLIYGRFNEALVVLLVAGITDALD
                     GAIARMKNQHTRFGAVLDPLADKLLLTSGFITLSMIHLIPLWVTILVVSRDVILLLGT
                     AVAHFTDSRIDITPTFLGKGTTFLQLSYVVVVIFLTSRHIDLTIM"
     gene            587..904
                     /locus_tag="VN444_00525"
     CDS             587..904
                     /locus_tag="VN444_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20329.1"
                     /translation="MSSLPLLFKREGLVEKHQLEGVDPSDRYFNRTVLVNRTASGYAA
                     KVMYEALIVEGHSHPTITAAVQELVESMQKLGFSKMRTRANFKGMKYLAEKETWVDYQ
                     DPA"
     gene            complement(914..>2115)
                     /locus_tag="VN444_00530"
     CDS             complement(914..>2115)
                     /locus_tag="VN444_00530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWR20330.1"
                     /translation="DPGFLLAAQQQIPAPMTDVLPMATSRVLQAALQSAVDDGVFPGA
                     QLAVRLRGELQCVAMAGRLSSEPFGAPVQACTIYDLASLTKPLATATSILLLIQAAKV
                     SLEDPVQQILAELEGTPIGQATVRELLTHRSGLPGWRPFYERLDARGIISGLSSRDQP
                     VKQQVLRMIRDEPLIYARGERSIYSDLGFMLLGLLVERLSGVALDRWCEEAIFRPLGA
                     DPLLFCPTAGQVQLEVGCSAVDISRIAPTEWDEWRNRLLCGEVHDENAAAMGGIAGHA
                     GLFGTAESVLVVSGAWLSGYHGRSSMLDGGLVRQFTARQELSAGSSWALGWDTPSAPS
                     SSGSFFSEESFGHLGYTGTSLWIDPRCELEVVLLSNRVHPNRRNEKIKTFRPYIHDLV
                     YREFVPV"
BASE COUNT          544 a          589 c          566 g          416 t
ORIGIN      
        1 acataatggt gagatcgatg tggcgggagg tcaagaatat tacgactaca acataggaca
       61 actggagaaa cgttgtgccc ttacccaaga aggtaggggt aatgtcgata cgagaatctg
      121 tgaaatgcgc caccgccgtt cccagcaaca gtatcacgtc ccggctgacc acgaggatgg
      181 ttacccacaa aggaatcaaa tgaatcattg atagcgtgat gaaccccgag gtcaggagta
      241 atttatcagc cagaggatcc aacacggccc cgaaccttgt atgctggttc ttcatgcgcg
      301 cgatcgcccc atcgagcgca tcagtgatcc cagccacgag aagcacgaca agcgcttcat
      361 tgaatctccc gtatatcagc agcccaatgt agcaggggat gaggagaata cggaggatgg
      421 tcaagctgtt ggggatattc atatcatctg gaacacagaa acccaccacg gcggggggag
      481 atgataggag ggcctttagg tagttgtcaa cggcttgcag tcagcaatgc cccgccctat
      541 aattcaaagt ctcagtctat cccaaccggg tatcacgggg gtcgtaatga gttctcttcc
      601 tctgctcttc aaaagagaag ggctggttga aaagcaccag cttgaaggag tcgaccctag
      661 cgatcggtat ttcaatcgga cggtccttgt caatcgaaca gcctcaggtt acgctgccaa
      721 ggtcatgtat gaagctctga tcgtcgaggg acactcccat ccaaccatca ccgccgcggt
      781 acaagaactg gtcgaatcga tgcagaagtt gggcttcagc aaaatgagaa cccgcgcaaa
      841 tttcaaaggc atgaaatatc tcgccgaaaa agagacctgg gtcgattacc aggaccccgc
      901 gtagccgagc ctgttacaca ggcacaaact cccggtatac caggtcatgg atatagggac
      961 ggaaggtctt gatcttttcg ttcctccggt tcggatgtac ccggttggac aacagcacga
     1021 cttctaattc gcaccgcgga tcaatccaca gtgacgtccc ggtataccct agatgcccga
     1081 acgactcctc ggaaaaaaat gaccctgatg atgacggagc tgatggagtg tcccatccca
     1141 acgcccaact cgatccggcg gacagttctt ggcgggctgt aaactgtctg acaagccctc
     1201 cgtccagcat cgaacttctt ccatgataac cgctgagcca ggccccggag acaaccagca
     1261 ccgattcggc agtcccgaat aacccggcat gaccagcaat accacccata gccgctgcgt
     1321 tttcgtcgtg cacctccccg catagtaacc gattgcgcca ctcatcccat tcggtcggcg
     1381 cgattcttga gatgtcaacg gccgagcatc caacttcgag ttggacttgc cctgccgtgg
     1441 ggcagaacag cagaggatcg gcccctaacg gccggaagat ggcttcttca caccaacgat
     1501 cgagcgcaac tccactgaga cgctccacca gtaatcctaa caacatgaat ccaaggtcgc
     1561 tgtaaatgct tcgctccccg cgcgcataga tcaacggttc gtcgcgaatc atgcgcaata
     1621 cttgttgttt gacgggttga tcccggctag aaagcccgga gataatccct cgtgcatcaa
     1681 gccgttcata gaaaggtcgc catcctggca gacccgagcg atgggttaag agctcccgca
     1741 cagtggcctg accgatcggt gtcccttcaa gttcggcgag tatctgttga acgggatcct
     1801 caagagaaac tttggcggcc tgaatcagga gcaagatgga tgtggccgtt gccagcggtt
     1861 tcgtgagaga agcgagatca tagatggtac aggcttgtac tggagccccg aagggctctg
     1921 aagacaaccg ccctgccatg gccacacatt gcaactcacc gcgcaggcgc accgccagtt
     1981 gggcaccagg aaatacaccg tcgtcaacgg ccgattgcag tgcggcctgg aggacacgag
     2041 aggtggccat aggcaagaca tctgtcatgg gcgcgggaat ctgctgctga gcggcgagaa
     2101 gaaaaccggg atcaa
//
LOCUS       DATMXI010000026         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_216949, whole genome shotgun sequence.
ACCESSION   DATMXI010000026 DATMXI010000000
VERSION     DATMXI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216949"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..232
                     /locus_tag="VN444_00535"
                     /pseudo
     CDS             53..232
                     /locus_tag="VN444_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
     gene            287..664
                     /locus_tag="VN444_00540"
     CDS             287..664
                     /locus_tag="VN444_00540"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HWR20331.1"
                     /translation="MKIQPTATHTIKEVFDHYSTEQDDYAFRRTHLLVALAQTEGASL
                     ISRSQAKRVMARCDRFREVSLDFRGVESIGPAFADEIFRVWRRAHPNITLAPSGMNKE
                     VEKMIRRALATDTPESPPQAQMG"
     gene            964..1764
                     /locus_tag="VN444_00545"
                     /pseudo
     CDS             964..1764
                     /locus_tag="VN444_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          534 a          637 c          657 g          442 t
ORIGIN      
        1 actacgccaa gacgctcgaa ctgcctcgag atatgcatat gaggagtaaa ccatggcgac
       61 atttgccccc ttcgatgctg ccgactatct cgatgatgag gaaaccatcg cggaatacat
      121 taccgccgca ctggaagacc ccaatcccga tgtatttctc actgccgtac gtgacgtggc
      181 ccgcgcccgt ggcatggcac aacttgccaa agatgccggt cttgggcgcg agggcgcaga
      241 gtagctcatt gatgacgcga agccactgtc tgggacctct gtcgcaatga agattcaacc
      301 gacggctaca cacacgatca aggaagtctt tgatcattac tcgaccgagc aggatgacta
      361 cgcatttcga cgaacgcatc tgctcgttgc gctagcacag acggagggtg cgtcgctgat
      421 ctctcgttcg caggcgaagc gagttatggc acgatgcgac cgctttcgag aagtcagcct
      481 tgatttccgt ggcgtcgaaa gcatcggacc tgcgtttgca gacgaaatct tccgtgtgtg
      541 gcggcgggct catcccaaca tcactcttgc tccaagcgga atgaacaaag aagtcgagaa
      601 gatgatccgc agagccttgg ctaccgacac tccagagtcg cccccacagg cacagatggg
      661 gtagcctgac ctcggttcga cgccacccca gtttcaagct gacccactac cgcccagcgc
      721 atgggttgcc tgtcgcatgc cgtttttgtt aatatctgga tcgatgattt ggatcgatcc
      781 tatagtttga ccactggagg aaacgcttcc cgctgagagc ctgtcctgcg accctcatat
      841 tgtgcttcga caggctcagc acgaacggag aatctcaatg tgttcactac ctataccgtt
      901 caccatgagc ttgtcgaatg gtaagcggga atttgtcgga caggctcttg aggggtggaa
      961 tgaatgacag aacctgagat accacgcgtg aagaaagccg gatacgatcc gcacgggatt
     1021 gaagagcact gggctaaagt ctgggaggcg gccggctaca gcaaggtcga tgagtcctcg
     1081 ccgaagccgc cgtttgccat tgtgatgccc ccgcccaata ttacaggctt cctccatatc
     1141 ggccatgcca tgaataatac cctccaggat atcctgatac gatggcgacg gatgcagggg
     1201 tataacgccc tctggatacc tggcaccgac catgcgggca tcgcgaccca aaacgtcgtt
     1261 gagcgacagc tccaggaaga gggacgtggg cgcgaggatc ttggccgcac tgggttcgtg
     1321 gaccgggtct ggcggtggaa ggcggaatcc ggcggaacga ttatccgcca gcttaagcga
     1381 ctgggtgtgt cctgcgactg ggaacgggaa tgtttcacca tggacgagga tcgccagcag
     1441 gcggtccgcg aggtgttcgt gcggttgtac gacgaggggc tgatctaccg aggcgagcgg
     1501 ctgatcaact ggtgcccacg ctgccaaacg gcgctctcag acatcgaggt agagcacgaa
     1561 gacacaaaag gcctcctgta tcatattgcc tatccgctcg cagatgatcc tgcgacagtc
     1621 ctcaccgtag ccaccactcg gcctgagacg atgctggggg acaccgccgt tgccgtccac
     1681 ccggacgacc cgcggtacaa tcgatttatt ggctgtcagg tgaggctccc gctgaccggg
     1741 cgaaccatcc cggtggtggg cgatgcggtc cttgtgaaaa tagggaaaat agggacagac
     1801 actattagaa aatagggaca gacactatta tcgtgataat tcgtggtgtt cgcggtcatg
     1861 atcgtgcctg ctgcggatca cccgaaccgg tgcatccggc tacccgtatc atgtgaggca
     1921 acgagggcat gatcgccagg cggtctttcg gaatgacagc gattcgaagg cgcacgatga
     1981 cagagcggcc actgcaaaga gacgggttca tccgggaggt gcgcagcaaa ctggatgagt
     2041 aatacacgtg tggcagaggg gaagaccaag aaaggatctc aataaagagg gtctgtccct
     2101 atatctcgca gccccgttag gcgcaccaag cacatacccg cgaggtgaga tcctggaatc
     2161 acagcagaca catcgacgag agtccatccc gaaaggaaac acgactacgc cgatgagcct
     2221 caccccccac cacgccaagt atttcgctca cgacctgacg cgtcgtgccg
//
LOCUS       DATMXI010000027         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_303168, whole genome shotgun sequence.
ACCESSION   DATMXI010000027 DATMXI010000000
VERSION     DATMXI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303168"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..427
                     /locus_tag="VN444_00550"
     CDS             71..427
                     /locus_tag="VN444_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWR20332.1"
                     /translation="MRDHIKLRAFELADEVAALIYRATRGFPKEEIYGLASQMRRAAV
                     SVPSNIVEGCARESQVEYLRFLEIAFGSLRELHYQFDSALRLGYTDEPGVCECDAKMM
                     ETEKVLGALIRSMRND"
     gene            689..1162
                     /locus_tag="VN444_00555"
     CDS             689..1162
                     /locus_tag="VN444_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWR20333.1"
                     /translation="MDRLRKKLIGRGNAGFTLIELMVVVAIIGILAAIAIPLYASMQA
                     KARVGRGQADAAVLAEAFSAFGAHCGDVPATVAAANWPAAGGGEAVATCAVNLALLGP
                     AALTRPSTDAAGISAGPFLRTVPQPLATWTYAYTRTGVGTFTVAAAGDGQNSTLP"
     gene            1273..1755
                     /locus_tag="VN444_00560"
     CDS             1273..1755
                     /locus_tag="VN444_00560"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HWR20334.1"
                     /translation="MVCRRNQVDGQLGFTLVELMVAIAVVGIVSVAAIPQFVTFLRAM
                     ETRGASQELATVLQQARELAIARNTNYRVEIEPDNNRLRFVRTSDNVVWTGPGTDAQG
                     YRRLVNQARLTNVTANPTFNPLGTAGGGTITVQNSQGTSSLNVVVSSAGRIRIAQVGQ
                     "
     gene            1767..>2119
                     /locus_tag="VN444_00565"
     CDS             1767..>2119
                     /locus_tag="VN444_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWR20335.1"
                     /translation="MRETRGFTLVEVLIALAILTVAILGVAATTALQSGGGSAGAVSF
                     GQAAVTRGYYLSTATMLAQDRLEQVKRVQYRAGTDPFTPGNPPPGFPDETPVNGFPNF
                     NRQVRVVAGPAADTRR"
BASE COUNT          437 a          584 c          652 g          446 t
ORIGIN      
        1 gaccgtatct acaccatgag taactactta gctagataga ctgtaggctg ttagactgta
       61 ggaacgcgcc ctgcgagacc atatcaagct cagagcgttt gaactggccg acgaagtggc
      121 ggccttgatc tatcgggcaa ccagggggtt tcccaaggaa gagatctatg ggttggcttc
      181 ccagatgaga agagcggctg tttcggttcc ttccaacatc gttgaggggt gtgcacgtga
      241 gagccaagtc gaataccttc gctttcttga aattgccttc gggtccttaa gggaactgca
      301 ttaccagttc gactcggccc tgcgcttggg ttatacggat gagccaggcg tttgtgaatg
      361 tgacgcgaaa atgatggaga ccgaaaaggt tctgggcgcc ttgattcggt ccatgcgcaa
      421 cgactaacag ccttcagcct acagcctaaa cacctgagta gctcccctaa aatcgtcagt
      481 caaccgacac ttttcgtcac ctcctcttcg tctttaagtg cctagatctc ttgctcttga
      541 tccttcctgc cctcttcgag tgctttactt ttcctggctt gatgatcagt aaggatatcg
      601 aagtacagat ggcacggtat atgctcacta acaacactaa atggttgttt ttgtatcgtt
      661 taaccttaga cggtaaagga gaagagcgat ggacaggttg agaaagaagc tgatcggacg
      721 tggtaatgct ggattcacat tgattgagtt gatggtcgtt gtggcgatca tcggcatcct
      781 ggcggcgatc gccattccgc tatatgccag catgcaggcg aaggcgcggg tcggcagagg
      841 ccaagccgat gcggcggtgt tggcggaggc attcagtgcc ttcggggccc actgcggcga
      901 tgtcccagca actgtggctg cggcaaactg gccggcagca ggcggggggg aggccgtcgc
      961 cacctgcgcg gtgaacctcg ctctccttgg accggcagcg ctcactcgcc cttcgacgga
     1021 tgccgcaggg atctcagcgg ggccgttcct tcgaacggtt ccacagccgc tggcaacctg
     1081 gacctacgca tatacgagga ctggggttgg gaccttcacc gttgcagcgg ctggcgatgg
     1141 gcagaacagc acgctgccgt aagcagccgc aagaggcgct actgcggagg ggagcaacta
     1201 ggcttgccac tgtgggacgg tgtgagggcc gtctcacggt ggtgagccat ctctgaagcg
     1261 aagggtgttc agatggtctg tcgacggaat caagtagacg gtcaactggg gtttaccctc
     1321 gtggagctga tggtcgctat tgcggtcgtc gggatcgtca gcgtggcggc tatcccccag
     1381 tttgtaacct ttctcagagc gatggagacg cggggagcat cccaagagct ggcgacggtg
     1441 ctccagcagg cccgcgagct ggccatcgcc cgtaacacca attaccgcgt agagatcgag
     1501 ccggacaaca atcgactccg ctttgtcagg accagcgaca atgtggtatg gaccggccct
     1561 ggaaccgatg ctcagggcta caggcgactg gtcaaccagg cgcgactcac caacgtgact
     1621 gccaacccga ccttcaaccc gcttgggacc gctggcggag gcaccatcac cgtgcagaac
     1681 tcgcagggca cgtcatccct gaatgtcgtt gtcagctctg cgggccgcat tcgaatagca
     1741 caagtcggcc agtgaggagg ggcgagatgc gagaaacgcg cggatttacc ctcgtcgagg
     1801 tcctgattgc cctcgcgatt ctcaccgttg ctatcttagg ggtggcggcg acgaccgcgc
     1861 ttcaaagcgg cggcgggagc gcgggggccg tctccttcgg ccaggccgcc gtcacgcgag
     1921 gctactattt gtcgacggcg accatgctgg ctcaggacag gctggaacag gtgaagcgag
     1981 ttcagtaccg ggcagggact gatcctttta ctccaggtaa ccccccgcca ggttttccgg
     2041 atgaaactcc ggtcaacggc tttcccaatt tcaacaggca ggtcagagtt gtggccggtc
     2101 cggccgcgga taccagacg
//
LOCUS       DATMXI010000028         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_84099, whole genome shotgun sequence.
ACCESSION   DATMXI010000028 DATMXI010000000
VERSION     DATMXI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84099"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN444_00570"
     CDS             <1..378
                     /locus_tag="VN444_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWR20336.1"
                     /translation="TDYAGLGWQRPVLAACMAVFMFSLAGIPPTAGFMGKLYIFSAAL
                     EGRYLGLAVIGVLNSVISVYYYLRVIVIMYMSEAASPRPLVPASVAAVLAVVMSVLGT
                     LHLGLFPAGLLDLARQSIAAVAG"
     gene            complement(829..1332)
                     /locus_tag="VN444_00575"
     CDS             complement(829..1332)
                     /locus_tag="VN444_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1150..1152),aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWR20337.1"
                     /translation="MNNPLRRAMYAALLIGILLVARTAHAAPVPDFTLPLLDGKSIAL
                     KDFRGKPVLINFFHSKUPHCQREAPVLAKIYQQYKGKGLVMLGVNTAWDNEADARRFV
                     ETYKVPYPVGRDSAGDIGRRYQIEGTPTTILVNKDGSLYGRSVGAMTEEEFQKSIDAL
                     LNQQQGK"
     gene            complement(1433..>2001)
                     /locus_tag="VN444_00580"
     CDS             complement(1433..>2001)
                     /locus_tag="VN444_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HWR20338.1"
                     /translation="VFHLTAVTHRRDPLYQTTIVGRPPMEDCHMGTAVERMSRPLLRK
                     QLPEIVDFHMPFAGVFHNLVIVSIDKSYPGHARKIMHAIWGLGQAMFSKVIVVVDKDV
                     NVRDPAEVTWKVLNHIDPERDIEFVMGPVETLDHASRLPRYGSKMGIDGTRKWKEEGF
                     TREWPEEQVMDKETRALVDQRWKEYGLG"
BASE COUNT          362 a          596 c          596 g          447 t
ORIGIN      
        1 accgactacg ccggtcttgg atggcagcgg ccggtcctgg ccgcctgcat ggctgtcttc
       61 atgttctcgc tggccgggat ccctcccaca gccggtttca tgggcaagct gtacatcttc
      121 agcgccgccc ttgaaggccg ctaccttggc ttggcggtga ttggtgtcct gaacagcgta
      181 atctcggtct actattatct ccgcgtcatc gtcatcatgt atatgagtga ggcagcgtcc
      241 ccgcgaccgc tcgttccggc atccgtagcg gccgtcctgg ccgtggtgat gtccgtactg
      301 gggactcttc accttggcct gttcccggca gggttgctgg atctggcccg gcagtcgatc
      361 gcagccgtcg caggatgaga gagccactgt ccatgaaagc gatcaagatt cccgagaaga
      421 ccgtgacccg gccaagattg gtcggcgagt tgaagggatc caggtgatgg ataccggggt
      481 aatcgtcccg gtgtcatccg caagcggaag atcaagatcg ggatcttagc cgttcccgcc
      541 gctcctgccc aggccgtcct cgacgcgctg gtggagggcg ggtcatcgcg gtcctgaagg
      601 ttcattcccc tcagcttgcc gcgagttcgt cataccggcg gaagccggat cggagtccgg
      661 ggcaggcgcc ggtatccaga gggcccgact ggattctgtc aagatccaga atgttgacct
      721 gtcagccccc ctgaagactt tgagctacca tatcgccagg acggagcggc ctacctgtgc
      781 gcgccgcaca caggtaggcc aagctccagg acgccgtgat gccctcgttt atttcccctg
      841 ttgctgattg aggagggcat cgatagattt ttgaaactcc tcctctgtca ttgccccgac
      901 cgagcgtcca tataagctcc cgtctttatt aaccaggatg gtggtcggcg tgccttcgat
      961 ttgataacga cggccgatgt caccggcgct gtcgcgtcct acgggataag gaaccttgta
     1021 cgtttcaaca aacctccgcg cgtcggcttc gttgtcccag gcggtgttca cgcccaacat
     1081 cacgagaccc ttgcccttat actgctggta gatctttgcc agaacgggag cctcccgttg
     1141 acagtgtggt catttggagt ggaagaaatt gatcaggacc ggcttgccgc gaaagtcctt
     1201 cagtgcgatg gactttccat ccaggagtgg gagcgtaaag tccgggacag gcgcagcgtg
     1261 ggccgtccgg gcaactaata gtatgccaat caggagggct gcgtacattg ctcgtcgtag
     1321 gggattgttc atgtctgatt cctcctaaat gtaggggcgc tgcttgctgc gccctctttg
     1381 ggcagggcaa gccccgcccc tacgccaaca actgatcact gatagcttcc catcaaccca
     1441 ggccgtactc tttccagcgt tggtccacga gggccctggt ctccttatcc atgacctgtt
     1501 cctccggcca ctcgcgcgtg aatccctcct ctttccactt acgcgttccg tcgatcccca
     1561 tctttgagcc ataccggggc agccgactgg cgtgatccag ggtttcgacc ggacccatga
     1621 caaactcgat atcgcgctcc gggtcgatat ggttcaggac cttccaggtc acttcggccg
     1681 gatcgcggac gttcacatcc ttgtccacga cgacgatcac cttcgagaac atggcctggc
     1741 cgaggcccca gatcgcgtgc atgatcttgc gcgcgtggcc tgggtaggat ttatcgatac
     1801 tgacgatgac gaggttatga aagaccccgg cgaagggcat atgaaagtcc acgatttcgg
     1861 gcaactgttt tctgaggagg gggcgggaca tccgttccac ggcagtcccc atgtgacagt
     1921 cttccatggg tgggcggccg acgatggtgg tctggtagag cggatcccgg cggtgggtga
     1981 cggccgtcag gtgaaagacg g
//
LOCUS       DATMXI010000029         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_104010, whole genome shotgun sequence.
ACCESSION   DATMXI010000029 DATMXI010000000
VERSION     DATMXI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104010"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VN444_00585"
     CDS             complement(<1..1403)
                     /locus_tag="VN444_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWR20339.1"
                     /translation="MDQRFSRRLLGVAGAISLSVSLLAPTFALSSEEPTRGHSLTIAP
                     QPLRHSGNQLTAAVVLPPGVHSVQPHSATVDSTSRTPSEDERVLRNGLVHSSRGMQAL
                     TAGDRAKAREELEISTSILTTISGGTTSGNLRLQLIEETALLKAALSGASPGQEVMSQ
                     PNDQEEEEDEADTEAAPDLVAPEEAQRLAPAEGDIVSIPELNVSGFDVPIVLNEQVKA
                     YILYYQTRKQGVMNRAFERSGRYLPMMRGIFRDQGLPLDLVNLAYIESTFNYRAYSKA
                     KASGIWQFIKGTGNRYGMKVNYWLDERRDPEKATKGAAAYLKELYGMFQSWPLALAAY
                     NAGEYRIQRAIEQQGTTDFWSLRLPKETQLFVPAFMAITIIAKDPARYGFTAPVEEPW
                     EVERATVPGAIDLRSVARVVGVPSDVIHDLNPALMRGVTPVTSVGHEISLPLGTKEIL
                     LANLDQLPRSSAREVIRA"
     gene            complement(1826..2152)
                     /gene="cutA"
                     /locus_tag="VN444_00590"
     CDS             complement(1826..2152)
                     /gene="cutA"
                     /locus_tag="VN444_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWR20340.1"
                     /translation="MESTTTYIVVLITTSSRQEAEAIGKVLVESRLAACVNISVGVTS
                     LFRWQGAIEHQEEVLMLVKSRSDLLPSITEVVKRLHSYTVPEVVALPILAGSPDYLAW
                     VDESLR"
     gene            2418..4334
                     /gene="dnaK"
                     /locus_tag="VN444_00595"
     CDS             2418..4334
                     /gene="dnaK"
                     /locus_tag="VN444_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWR20341.1"
                     /translation="MPKVLGIDLGTTNSVMAIMEAGDPVVVPNAEGGRLTPSVVAFSK
                     EGERLVGQVAKRQATTNPENTIFSIKRFMGRRNEEVSQEIKLVPYKVVRASNGDVGVE
                     VRGKSYSPPEISAMILQKMKTDAEAYLGEKITQAVITVPAYFNDSQRQATKDAGKIAG
                     LEVLRIVNEPTAAALAYGLDKKRDEKIAVYDLGGGTFDVSILELGEGVFEVKSTNGDT
                     HLGGDDFDEQIISWLVDEFKKDQGIDLRKDRMALQRLKEAAEKAKCELSTVLETEINL
                     PFITADASGPKHLSLKLTRAKLEQLVEDLIQRSIGPCRQALKDAGLDPSNIDEVVLVG
                     GQTRMPKVQALVRELFGKEPHKGVNPDEVVAVGAAIQAGVLVGDVKDVVLLDVTPLSL
                     GIETLGGVMTRLIERNTTIPTRKSELFTTAADSQTSVEIHVLQGERQLARDSRTLGRF
                     HLVGIPSAPRGIPQIEVAFDIDANGILNVAAKDLATSKEQKITITASSGLTKDEIERM
                     VKESDRCAEEDKKRREEIEVRNQLDSLCYQTEKMLNENREKLPIAELGTLETAIEAAK
                     EALKGEDTGKQREALDQLTKASHRLAELLYQQAQSKQTAPGEQAQSEPSQEAPEGGVV
                     DAEFEDLGGKGDKK"
     gene            4357..4809
                     /locus_tag="VN444_00600"
     CDS             4357..4809
                     /locus_tag="VN444_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWR20342.1"
                     /translation="MAIVRWDPFRDVTTLQERMNRLFDQALSRTRMDDDEGLTASMWS
                     PAVDIFETPDSIVMKAELPGVSRDNIDIQVQDNTLTLKGERKFEREVQEENYLRLERS
                     YGAFQRAFSLPTGVQQDKIMAVFKDGVLEVTMPKAEEAKSRQVKIDVK"
     gene            4978..>5260
                     /locus_tag="VN444_00605"
     CDS             4978..>5260
                     /locus_tag="VN444_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWR20343.1"
                     /translation="MNKRDYYEILGVRRGASDKEIKQAYRRLARKHHPDVNPNNKAAE
                     AKFKEITEAHEVLSDPAKRQQYDQFGRQPFGVGHEADQRPGAGPGGFDFS"
BASE COUNT         1239 a         1433 c         1580 g         1008 t
ORIGIN      
        1 gctcgaatca cctcccgtgc agaggatctc ggcaactgat ccagattagc caggaggatc
       61 tccttggtgc caagcggcaa agagatctca tgcccaacgg aggtcacagg ggtgacccca
      121 cgcatcaggg ctgggttgag atcatggatg acatcggacg gaacgcctac cactcgagca
      181 acggaacgca aatcaatggc cccgggcacc gtcgctcgct ctacctccca tggctcctca
      241 accggcgcag tgaaaccgta ccgagctggg tccttggcaa tgatggtaat ggccatgaag
      301 gccgggacaa agagctgggt ttctttcgga agccttaacg accagaaatc ggtggttccc
      361 tgctgttcga tggctcgctg aatccgatac tcccctgcgt tgtaggcggc cagcgccagt
      421 ggccaggatt gaaacattcc gtacagctct ttcaggtagg cggctgcccc tttggtcgcc
      481 ttttccggat cccgccgttc atcaagccag tagttcacct tcatcccgta ccgattcccg
      541 gttcccttga tgaactgcca gatccccgag gccttcgcct tggagtaggc gcgatagtta
      601 aaggtacttt cgatatacgc cagattgaca agatccagag ggaggccctg atcccgaaag
      661 attccgcgca tcatcggcag gtaccgccct gaacgctcga aggcccggtt catcaccccc
      721 tgctttcgag tttgatagta caggatgtac gccttgactt gctcgttgag tacgatcggc
      781 acgtcaaagc cgcttacgtt cagttcagga atcgaaacga tatcgccctc tgcgggcgcc
      841 agcctctgcg cttcttcggg ggcgacgagg tcaggggccg cctccgtatc ggcttcgtcc
      901 tcttcctcct cctggtcgtt tggctggctc atgacctctt gacctggaga agcgccagaa
      961 agtgccgctt tgaggagggc cgtttcttcg atgagctgga ggcgaaggtt acccgaagtt
     1021 gttccccctg agatcgtcgt caggatactc gttgagatct ccaactcttc ccgggccttg
     1081 gctcgatccc ctgccgtcaa tgcttgcatc cccctggaag aatggaccaa cccattccga
     1141 aggaccctct cgtcttcgct gggcgttcga gacgtgctgt caacggttgc gctatgaggt
     1201 tggacgctat gaacaccagg aggcaagacg acagcggctg tgagctgatt accagagtgg
     1261 cgtagcggct gtggggctat agtgaggcta tgaccacggg tcggctcctc ggaagataag
     1321 gcgaaagtag gggcaaggag cgacacgctc aacgaaatag cgcccgctac ccctaggagc
     1381 cttcgggaga atcgctggtc catcttgacc tcctgttagg gttattagcc caaagccggc
     1441 aagcatcaag aagccgtggt gattcgttct ttctgcagag ccccaacagc gacgggcgat
     1501 tgtagggctt tatacccttc ttcgatcggt ttgtcaagca tttcatctga ggaagctatc
     1561 agctttcagc ggtcagtctg atgcgttccc cgcttcatct gaggctgaag gcgatttcct
     1621 gaggaatagt catggctctt cgggccaccc atgccttatg aaaccccgcc aaccccccct
     1681 ttagcaaagg ggggggcacg gggggatttg agtcgtgggc gatcttctga gcaacttaga
     1741 gcaagggtcg tgccaaggtc aactcagcct gaagataggg aagccgggtc gccgtggatg
     1801 gtgacctctg gcgagcctcg gggcgtcacc gtaacgactc gtccacccaa gcgaggtagt
     1861 cgggggaccc ggcgagaata ggtaatgcca ccacttccgg tacagtgtaa gagtgtaacc
     1921 tttttacgac ctcagtaatg gagggaagga gatcgcttcg gctcttcacc agcatgagca
     1981 cctcctcctg gtgctcgatc gctccctgcc accgaaagag agaggtcacg ccaaccgaga
     2041 tgttcacgca ggctgccagt cgggactcga ccagcacctt gccgatcgcc tcagcttcct
     2101 gcctggaact tgttgtgatc aggacaacga tataagtagt tgtcgattcc atcccccctc
     2161 ctcaaataaa gaccgtagac cgagtattct ccttagtatt caatggatta tagcgacatt
     2221 accccacgcg atacggcggg tcaaggggtt ttccaactgg agatgctaga aaagggaaag
     2281 tggaaatcta aaagaattct tgcaatgata gttgacaggt acagactaat gtactatctt
     2341 tgcatacgag aatagtgaag agcagatcca tgcttggttt aattgactgt gacaatttct
     2401 tatcaggagg gtaagaggtg ccaaaagttc tcggtattga cctcggaaca accaattccg
     2461 taatggccat catggaggct ggagacccag tcgtggtccc caatgccgag ggtggtcggt
     2521 tgaccccttc ggtcgtggct ttctcgaagg aaggggagcg actggtgggt caagtcgcca
     2581 agcggcaggc aaccaccaac cctgaaaaca cgatcttttc tattaagcgc ttcatgggtc
     2641 gacgtaatga ggaggtcagc caggagatca agctggtacc gtacaaggtg gttcgggcca
     2701 gcaatggcga tgtcggggtt gaggtaagag ggaagagcta ttcgccacca gagatctccg
     2761 ccatgatcct ccaaaagatg aagacggatg ccgaggccta tctcggggag aagatcacgc
     2821 aggcggtaat caccgttccc gcctatttta acgactcgca gcgccaggcc accaaggatg
     2881 ccggcaagat cgccggcctc gaggtgctcc ggatcgtgaa cgaacctacg gcggcggcgc
     2941 tggcctacgg tttagataag aaaagggatg agaagattgc cgtctacgac ctgggagggg
     3001 ggacattcga cgtgtcgatc ctggagttgg gtgaaggggt ctttgaagtc aaatctacca
     3061 acggcgatac ccacctcgga ggcgacgact tcgacgagca gatcatctcg tggctggtgg
     3121 atgagttcaa gaaggatcag gggatcgacc tgcgtaagga ccggatggcg cttcagcgcc
     3181 tgaaagaggc tgcggaaaag gccaaatgcg aactcagcac ggtgctggag actgagatca
     3241 acctgccatt catcacggcc gacgcctctg gcccgaagca cctgagcctc aagctcacga
     3301 gggcgaaact cgaacaactg gtcgaggacc tgattcaaag gtccatcggg ccctgccgtc
     3361 aggcattgaa ggacgcaggt ctcgatccaa gcaacatcga tgaggtcgtg ctggtaggcg
     3421 gacagaccag gatgccgaag gttcaagcgc tcgtgcgcga gctctttgga aaggagccgc
     3481 ataaaggggt gaatcccgat gaggtggtcg cggtcggcgc agccattcag gccggcgtgt
     3541 tggtcggtga cgtcaaagac gtggtcctct tggatgtgac gcctctgtcg ctggggatcg
     3601 aaaccctggg tggggtgatg actcgactga tcgaacggaa caccaccatc ccgacccgca
     3661 agagcgaact cttcacgacg gcagccgata gtcagaccag cgtggaaatc catgtcctcc
     3721 agggggagcg gcaactggcc agagacagcc ggacgctggg ccgtttccac ctggtgggaa
     3781 tcccatcggc accgcggggt attccacaaa tcgaggtcgc cttcgacatc gacgccaacg
     3841 ggatcctcaa cgttgccgcc aaggaccttg ccaccagcaa ggagcagaag attaccatca
     3901 ccgccagctc cggactcacc aaagacgaga tcgagcgaat ggtcaaagag tccgatcggt
     3961 gcgccgagga agataagaag cggcgcgagg agatcgaggt ccggaaccag ctcgacagtc
     4021 tgtgctacca gacagagaag atgctgaacg agaaccgcga aaagctcccg atcgccgagc
     4081 tgggcacgct ggagaccgcc atcgaggcgg ccaaggaggc cctgaagggc gaggatacgg
     4141 gaaagcagcg cgaagcgctt gatcagctta ccaaggcctc ccaccggctt gctgagttgc
     4201 tctatcagca ggcccagagc aagcaaactg ccccaggtga gcaggctcag agtgagccat
     4261 cgcaggaagc gccagagggt ggggtagtcg atgccgagtt cgaggacctc ggcggtaaag
     4321 gcgacaagaa ataagagaag aaaggaggtg aagcaggtgg cgattgttcg atgggatcca
     4381 ttccgcgatg tcacgacgct tcaggaacgg atgaaccgct tgttcgatca agcactgtcc
     4441 cgaacccgca tggatgatga tgaggggctg acagcctcca tgtggtcgcc cgctgtggat
     4501 atcttcgaga ctccagacag catcgtcatg aaggcagagc tgcccggggt gagccgggac
     4561 aacatcgaca tccaggtgca ggacaacacg ctaacactga agggcgagcg aaagttcgag
     4621 cgggaggttc aggaggaaaa ttacctccgg ctcgagcgct catacggggc gtttcagcgc
     4681 gccttcagct tgcccaccgg tgtccaacag gacaagatca tggcggtgtt caaggatggt
     4741 gtactggagg tcaccatgcc gaaagcggaa gaggccaagt ccaggcaagt gaagatcgac
     4801 gtgaagtgat aggaagcgaa aagtaactac gaggtagata ggctgaaggc cgaaggcttg
     4861 taggggtaga aaccgacttc agccttcagc ctaaacacct aactcgattc gtgccttgcg
     4921 agatcatgta gagccttcag ccttcagcct aaccgcccaa gtagttacgg tgcaagaatg
     4981 aataagcgcg actactatga gatccttggc gtccgcaggg gtgcctcgga taaggagatc
     5041 aaacaggcct accggcgcct ggccaggaag caccaccctg acgtcaaccc caacaacaaa
     5101 gcagccgagg cgaagttcaa agagatcacc gaggcgcacg aggtcttgag tgacccggcc
     5161 aagcgccaac agtacgacca gttcggacgc cagccatttg gcgtggggca tgaggcagac
     5221 cagcggcccg gagcagggcc gggtgggttc gactttagcc
//
LOCUS       DATMXI010000030         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_191379, whole genome shotgun sequence.
ACCESSION   DATMXI010000030 DATMXI010000000
VERSION     DATMXI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191379"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VN444_00610"
     CDS             <1..1266
                     /locus_tag="VN444_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HWR20344.1"
                     /translation="RLEGAGFDAVIGNPPYVRQEGLGDDKPFFEVAHAPVYSGVADLY
                     VYFYHRGLSVCRKGGRFGMITSNKFLRAGYGKALRTFLAGHAVTDIIDFRDLPVFPDA
                     TAYPLILVARREPPPEQHRVRGCTVGSMAEASQIEEVMSRQGPSMPIAALREEGWALQ
                     RPEVLHLLEKIRAAGRPLGEVIGGKFYYGIKTGYNEAFVIDDAKRAELIAADPNTAEI
                     IKPFLRGRDVKRWRVEWAGLYLIKTEIGVNIKRYPVIFEHLKQYQPALEKRWDKGKYW
                     WELRACDYYAEFEKPTIRYQVIATYQQFAFTNEPYLSNDKTWIIPTDDPTLVAILNSK
                     LAWWFLDHIVAKLQGGAFELRSTYVEQIPVPAIDNKDRRALTEHAKFILAAPDDTGAV
                     AEHEQAINEIVYRLYGLTDHEIALVEGLG"
     gene            1367..2008
                     /locus_tag="VN444_00615"
     CDS             1367..2008
                     /locus_tag="VN444_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR20345.1"
                     /translation="MPDQEHCRRRSLRLQGYDYSQAGAYFVTICTQGRKCRFGDIVNG
                     QMHLNEVGRAVHSVWERLSARFPTLETDTFVVMPNHIHGILVLVGAGLALPVEGAASS
                     APTKTTSITLSDVVRAFKSISAIDVNRLLSRLGQPLWQRGFYEHVIRNDESLNRVREY
                     IATNSLRWGLDRENPQRTGRDDFDDWLRTFTIRPERVEIVVESPTSLITRPIV"
     gene            2059..>2741
                     /locus_tag="VN444_00620"
     CDS             2059..>2741
                     /locus_tag="VN444_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20346.1"
                     /translation="MKFSLPEIRHDLAGFDALVRLNAATEDLQFSTIDIDMATTSWFD
                     ADMCAPFGAILYRIGHHMNTVRLTSLQPGIERILSKNGFLSHYGRVPLRDTYGTTIPY
                     QRFDVKDDRFFASYVESEFVQRSEIPRMTPGLMKRFRESVYEIFSNAVIHSQTELGIF
                     SCGQFFPKRNSLDFSVADLGIGIRRNVRERAGLDLPADKAIVWATEERNTTKRGPIPG
                     GLGLKLLSE"
BASE COUNT          597 a          770 c          777 g          597 t
ORIGIN      
        1 cggctggagg gcgcggggtt tgacgccgtg atcggcaatc cgccgtacgt gcggcaggag
       61 ggattaggcg acgacaagcc attcttcgag gtagcccatg ccccggtcta ttccggggtg
      121 gccgacctct acgtctattt ctaccatcgt ggcctgtccg tctgccgcaa gggtggtcgc
      181 tttggcatga ttacctccaa taagtttctg cgtgcaggct atggcaaggc gctacggacc
      241 ttccttgccg gccatgccgt cacagacatc attgacttcc gagacctgcc ggtcttcccg
      301 gacgcgacag cctatccgct catccttgtc gcacggcgtg aaccgccgcc ggagcagcac
      361 cgtgtccgcg gttgcacagt ggggagcatg gctgaagcct cacagatcga ggaagtcatg
      421 agccgtcaag gcccatcgat gcccatcgcc gctttgcgtg aggaaggctg ggcactgcag
      481 cgtccggagg ttctgcatct cctggagaag atccgcgcag ccggacgacc gctgggagaa
      541 gttatcggcg ggaagttcta ctatgggatc aagacaggtt acaacgaggc gttcgtaatc
      601 gacgacgcga aacgtgccga gttgatcgcc gctgatccga acaccgccga gatcatcaag
      661 ccgttcctac gcggccgcga cgtcaaacgc tggcgggtgg agtgggcggg gttgtacctg
      721 attaaaactg agattggcgt gaacatcaaa cgctatcccg tgatctttga acatctcaag
      781 caataccagc ccgctctcga gaaacgatgg gacaagggaa agtattggtg ggagcttcgg
      841 gcgtgtgact actacgccga gttcgagaaa ccgacaattc gttatcaagt gatcgcgacc
      901 taccagcaat ttgcgtttac caacgaaccc tatctctcta acgataagac ctggatcatc
      961 cccaccgacg acccgacgct tgttgccata ctgaactcta agctggcgtg gtggtttctg
     1021 gatcacattg ttgctaaact ccagggcggt gcgtttgagc tccgttccac ctacgttgag
     1081 caaattccgg ttccagccat tgataacaag gatcggcgtg cgctgacaga acatgccaag
     1141 ttcatcctgg ccgcgcccga tgataccggt gccgtggccg agcacgaaca ggccatcaac
     1201 gagatcgtct accgcctgta tggcttgacg gaccatgaaa tcgcactcgt ggaggggttg
     1261 ggatgaccga gaatatcgtc cccgtacctg tcgtaggggc agggcttgcc ctgcccgagg
     1321 cgggcgcagc cagcagcgtc cctacagccc ctgaaggaac gcgttgatgc ctgaccaaga
     1381 acattgccga cgtcgatccc ttcgtctcca ggggtatgat tattcccagg ctggagccta
     1441 ttttgtgacc atctgcacgc agggccggaa atgccggttt ggggatatcg tgaatgggca
     1501 aatgcacctg aacgaggtgg gtcgggcagt ccacagtgtt tgggaacggc tctcagcacg
     1561 gtttccaact ctcgaaacag atacttttgt tgtgatgccg aatcacatcc acggcattct
     1621 tgtgcttgta ggggcagggc ttgccctgcc cgtcgagggc gcagccagca gcgcccctac
     1681 aaaaaccaca tccatcacgt tgagtgatgt agtgcgggcg ttcaaatcca tatccgcgat
     1741 cgacgtgaac cggctcttgt cacgattggg tcagcctctc tggcagcgag gcttttacga
     1801 acacgtcatt cgcaacgacg aatctctgaa tcgtgtccgc gaatacatcg cgactaactc
     1861 cctccgttgg gggctcgacc gagagaatcc ccagcgtacc ggccgggatg acttcgatga
     1921 ctggctgaga acattcacga tccgtccaga gagggtcgaa atcgtagtag aatctcctac
     1981 gtctttgata accagaccca ttgtctgaca gacatcctga atgtccttga gacaggatat
     2041 atcaccagga atagcaggat gaaattctcg ttgccggaaa ttcgtcacga cctcgccggt
     2101 tttgatgcat tagtccgcct gaatgctgca accgaggatc tgcagttttc cacgattgac
     2161 atcgacatgg cgactacctc ctggttcgat gcggacatgt gcgcaccgtt tggcgccatt
     2221 ctctatcgga tcgggcatca catgaatacc gttcgattga ccagtctgca accggggatt
     2281 gaaagaattc tttccaaaaa cggatttctc agccattatg gacgcgtgcc cctccgggac
     2341 acctatggca ccacgatccc gtatcagcgc tttgacgtga aggacgatcg attctttgcg
     2401 agctatgtgg aatcggagtt cgtccaaagg tcggaaatac cccgcatgac gcctgggctt
     2461 atgaaacggt tccgcgagag tgtgtatgag atcttcagca acgcggtcat ccattctcag
     2521 acagagctag gcatcttcag ttgtgggcag tttttcccca agcggaacag cctcgatttt
     2581 tctgtggctg acctcggtat cggcattcgg cgcaacgtga gagagcgcgc cggcctcgat
     2641 ctgccggccg ataaggcgat tgtctgggcc acagaagagc ggaatactac aaaacgaggc
     2701 ccgattcctg gcgggctcgg cctcaagcta ctgagcgagt t
//
LOCUS       DATMXI010000031         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_83847, whole genome shotgun sequence.
ACCESSION   DATMXI010000031 DATMXI010000000
VERSION     DATMXI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83847"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /gene="secD"
                     /locus_tag="VN444_00625"
     CDS             <1..1536
                     /gene="secD"
                     /locus_tag="VN444_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWR20347.1"
                     /translation="YYLFPTLSGRSSLPSLLPPLLPRAERLNLGLDLQGGMHLVLEVV
                     TEKAVENTVDRLIAELRRETEKEKITVEQMTREGIDRIRIRLQAKEGMDGLRRILKNY
                     SNLQQSGAADPAELSLSLTSGEMKRIQESAIRQSLETIRNRIDQFGVAEPHIVQEGDR
                     RIVVQLPGIKEPQRAINLIGKTALLEFKLVVEDANIAEAVAGKIPEGDELLYVRRTDE
                     QDKTSKAPLVVQKRAILTGDLLTSAQVQIEGQTNEPVVSIAFDQEGARLFGEATAANV
                     GRRLAIVMDGTIYSAPVIREKIPGGKAQISGRFTMEDARDLAIVLRAGALPAPVNIVS
                     NLTVGPSLGLDSIEKSVRAAILGGALVIVFMAIYYRLSGVIANFALLLNLIWLLGALA
                     SLRATLTLPGIAGIILGIGMAVDSNVLILERIREELRLGKAVRSAIDGGYDKAFLTII
                     DSHVTTLITAFALFLFGTGPVKGFAVTLSLGVIFNLVTALTGTRVVYDWITQRWNLKT
                     LSI"
     gene            1643..2581
                     /gene="secF"
                     /locus_tag="VN444_00630"
     CDS             1643..2581
                     /gene="secF"
                     /locus_tag="VN444_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWR20348.1"
                     /translation="MIEILGKTKIDFIAWRRTAFAVSAVLCLLGMISIIQIWRGAANL
                     GIDFAGGTSVQLKFSRPVDLGQVRTLLAESGLKDSDPQQFAGGDRIMVRLKRAEGSQA
                     GMAQRVQDIFSKGLPDNPFVVEGTNEVGPAIGKDLQKAALWAIISSMIGIMTYIAWRF
                     EFKFGVAAAIATLHDVLAVLGFFYVLNGEITLLIITALLTLAGYSLTDTVVVFDRIRE
                     NLQGRRKESLGDVINMSINEVLSRTIVTSLTVLLVLLSLFILGGEVLHDFSLALIAGV
                     CVGTYSSWFVASSIVYEWRTWERARLKAPVKAKAKG"
     gene            2791..>2971
                     /locus_tag="VN444_00635"
     CDS             2791..>2971
                     /locus_tag="VN444_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HWR20349.1"
                     /translation="MESGTSHSHRDIAEAIFRESAEIKLAFLEGHLDLVVRAAAAIAG
                     ALRAGRKILIFGNGGS"
BASE COUNT          619 a          791 c          903 g          658 t
ORIGIN      
        1 tactatctct ttcccacgct atccggccgg tcgtcactcc cctcgttatt gccgccgttg
       61 cttccgcgcg ctgaacgact caaccttgga ctcgatctac agggagggat gcacctggtc
      121 ctggaggtcg ttaccgagaa ggcggtggaa aacaccgttg accggctgat tgccgaattg
      181 cgccgggaaa ctgagaagga gaagattacg gtcgagcaga tgacccgcga ggggatcgac
      241 cgtatcagga tcaggcttca ggcgaaggaa ggtatggatg gcttacgccg tatcctgaag
      301 aactatagca acctgcaaca gtccggtgcg gctgatccgg ctgaactcag cctgagcctc
      361 acatcgggtg aaatgaagcg gatccaggaa tccgctatcc gacagagcct ggaaactatc
      421 cggaatcgca tcgatcagtt cggcgtagcc gagcctcaca tcgtacagga aggggaccgg
      481 cggatcgtag tccagcttcc aggtatcaag gagccgcagc gggcgatcaa tctgatcggt
      541 aagaccgccc tcctggagtt taagctggtg gtggaggatg ccaatattgc tgaggcagtg
      601 gcgggaaaga tcccggaggg cgacgagctt ctctacgtac gccgtacgga cgagcaggac
      661 aagacgagca aggctcccct tgtcgtgcag aagcgagcca tcttgacggg tgatctgctc
      721 acctcggctc aggtgcagat cgagggccag accaacgagc cggtggtctc aatagcattt
      781 gatcaggagg gtgcccgtct ctttggcgag gccacggcgg ccaacgtcgg ccgacgcctg
      841 gccatcgtca tggatggcac catctattcg gcgccggtca tcagggagaa gatcccaggc
      901 ggcaaggccc agatctccgg gcgcttcacc atggaggacg ccagggacct ggcgatcgtg
      961 ctacgggccg gcgcgcttcc cgcgcccgtt aacatcgttt cgaatctgac ggttggccca
     1021 tccttgggcc tggactccat cgaaaagagt gtgcgggccg caattctggg cggcgccctc
     1081 gtcattgtct tcatggcaat ctactacaga ttgtccgggg tcatcgccaa ctttgcgcta
     1141 ctcttaaatc ttatctggct gttgggggct ctggccagcc tcagggccac gctgacgctc
     1201 cctggcattg cgggcatcat ccttggaatc gggatggcgg tggactcgaa cgtactgatt
     1261 ctggagcgga tccgtgaaga actccgattg gggaaggcag tccggtcggc cattgacggg
     1321 ggctacgaca aagcattcct caccattatc gactcccatg tgacgacgtt gattaccgcg
     1381 tttgcgttgt ttttgttcgg gacggggccg gtgaaagggt tcgcggtcac cctgagcctc
     1441 ggggtaatct tcaacctggt gacggccttg acgggaacca gggtggtgta cgattggata
     1501 acccagcggt ggaacctgaa aacccttagt atctagggga tagggtggag agggtatagg
     1561 gtttagggta tagggaaaag cggtggcacg caggctccta aactataccc tatacgctat
     1621 accctagacc ctgaggtctt atatgattga gattctcggt aagacaaaga tcgatttcat
     1681 tgcctggcgg aggactgcgt tcgcggtttc tgcagttctc tgccttctcg gaatgatctc
     1741 gatcattcag atctggcgcg gcgcggcgaa tctcgggatt gacttcgccg gcggcacctc
     1801 cgttcagctc aagttcagta ggccggtgga tcttggtcaa gtcagaacgc tgctggcaga
     1861 aagtggcctg aaggatagtg atccccagca gttcgccggt ggggaccgca tcatggttcg
     1921 tttgaagcgg gcggagggca gtcaggccgg aatggcccag cgggtgcaag atatcttctc
     1981 aaaagggtta cctgacaatc cgttcgtggt agaggggacg aatgaggtcg ggccagccat
     2041 cggaaaggac ctccaaaaag ccgccctgtg ggccatcatc agttccatga tcggcatcat
     2101 gacctatatt gcctggcgct ttgagttcaa attcggggta gcggcggcca tcgccaccct
     2161 gcatgatgtc ctggctgttc tggggttttt ttatgtgttg aacggggaga tcaccctcct
     2221 catcatcact gcgttgctta ccctcgccgg ctattcgctg acagataccg tggtggtctt
     2281 cgatcggatt cgtgagaatc ttcaaggccg acggaaagaa tcgctgggag acgtcatcaa
     2341 catgagcatc aatgaggtct taagtcgaac tatcgtgacg tcgctcacag tccttctggt
     2401 cttgctctcc ctcttcatac ttggcgggga ggtactccac gatttctctc tcgcactgat
     2461 cgccggtgtc tgtgttggca cctactcttc atggtttgta gcctcctcaa ttgtctatga
     2521 gtggcgtacg tgggagcgag ccagactgaa agctcccgtc aaggccaagg ccaaggggta
     2581 aacggaagtc gcagacccac gatgtgggga cgcttcgact tttcccttga caactcaact
     2641 cgccataagg tagtacctcc aatagagcta gaattggtgt ggtacaactg tcaattccaa
     2701 acgccaggtt ccgcgttcca cgctgtggac ctgtgaggtt tgagatgcta cctggaactt
     2761 ggagcggctt ttcaacctgg aacccggaac atggaatctg gaacctctca ttcgcatcga
     2821 gacattgccg aggcgatatt ccgtgagagc gctgagatca agcttgcctt cctggaaggc
     2881 catctggact tggtggtccg ggcggcggcg gccattgcgg gggccctacg agccggaagg
     2941 aagatattga tcttcggtaa cggcggaagc g
//
LOCUS       DATMXI010000032         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_16178, whole genome shotgun sequence.
ACCESSION   DATMXI010000032 DATMXI010000000
VERSION     DATMXI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16178"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VN444_00640"
     CDS             complement(<1..944)
                     /locus_tag="VN444_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR20350.1"
                     /translation="MPNVSVSSDLSSDALPVLIQGAFQGFTGAAERLEQAYAALREQV
                     ATLNFALNDTNLRLTQSLQENARIRYYLSNLLKSIEKGIVVVDAAGRITLWSGGAERL
                     TGFLAAEVLGEQADRLLGGEAATLLHGVTGDGREGAREGRIMRKDRYILEAEISASPI
                     YDESGRVLDTIVIFDDISQRKWVEERRRRSTAQAGLEQMAVTIAHGIRNPLTSIELLA
                     TILAEEAGADVRQTHLAQGIQAGVASVNIILINLLAFTRPVKPCLRPLDLHEVIEEAL
                     GTAWYALKERQIDLIREYESGLLEVDGDRELVIQVFLNL"
     gene            complement(983..1351)
                     /locus_tag="VN444_00645"
     CDS             complement(983..1351)
                     /locus_tag="VN444_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20351.1"
                     /translation="MRVLIVDDSATMRRIIRNNLKFAGYDDAVEAGNGVEGLACLTGN
                     PIDLIITDWNMPEMNGIEFVKAVRSKDQLKQLPVLMITTVAERENIMIALAAGVTNYI
                     VKPFDAETLKKKLDQVLAAA"
     gene            1981..>2540
                     /locus_tag="VN444_00650"
     CDS             1981..>2540
                     /locus_tag="VN444_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HWR20352.1"
                     /translation="MLAIIGVVVVFGSVIGGFAIEGGPIPVLIQPVEFLIIGGASIGG
                     LLIAVPIKILKILIRQIMGTLGSGLGKQAYLEVLQVLYDLFTKAKREGFTAVDSDLMS
                     PEKSSIFSKYKGFIKNHHAMTFLCDSLRLVVDGAVDADELEAIMDTELETHHEEGGQT
                     PSIMSKVGDSLPGLGIVAAVLGIVIT"
BASE COUNT          559 a          757 c          677 g          547 t
ORIGIN      
        1 agattgagaa acacctgtat caccagctcc cgatcgccat caacttcgag aaggcctgac
       61 tcatactctc tgatcagatc gatctgacgc tccttgagcg cataccacgc cgtgccgagc
      121 gcctcctcaa tgacctcgtg cagatcgagg ggtcgcaagc agggcttgac aggtctggta
      181 aaggccaaca gattgatcag gatgatgttc acggaggcga cacccgcctg gatgccttgc
      241 gccagatggg tttggcgaac atcggctcct gcctcttcgg cgaggatcgt agccaacagc
      301 tctatcgagg tcagcggatt tctgatcccg tgagcaatcg taaccgccat ctgctctaac
      361 ccggcctgcg cggtcgatcg ccgtcgtcgc tcttcaaccc atttccgctg cgagatgtcg
      421 tcgaagatta caatggtgtc cagaactctt ccgctttcat cgtagatcgg cgacgcactg
      481 atctcggcct ccagaatgta tctatctttc ctcatgatcc gcccctccct ggcgccttca
      541 cgcccgtctc cggtcacgcc atggagaagg gtcgctgcct ccccacccag aagacgatcg
      601 gcctgctccc ccaacacctc ggcagccagg aagccggtca gtcgttctgc gcccccgctc
      661 catagcgtaa tccggccggc ggcatcgacg acaacgatcc ctttttcaat actcttcaga
      721 aggttggaca ggtagtagcg aatacgggcg ttctcctgca ggctttgggt cagccggagg
      781 ttcgtatcat tcagtgcgaa gttgagagtc gctacctgct cccgaagagc ggcgtaggcc
      841 tgctccagcc gctcggccgc cccagtaaat ccctgaaagg ccccctgaat caggaccggg
      901 agcgcatcgc tggagaggtc ggaactgact gacacgttag gcacgggcgt ctcctattcc
      961 agtgataagg caagcgcggc ccttacgcag ccgccaggac ctgatcgagc ttctttttga
     1021 gggtctcagc gtcgaacggt ttgacgatat aattcgtcac ccccgcggcg agcgcgatca
     1081 taatattctc ccgctccgcc actgtcgtaa tcatcagaac cggcaactgt ttgagctgat
     1141 ctttcgaacg aacagccttt acaaactcga tcccgttcat ctccggcatg ttccagtctg
     1201 tgatgatcag atcgatcggg ttgcccgtca gacaggccaa gccttccact ccatttccag
     1261 cctctaccgc atcgtcgtat ccggcaaact tcaggttatt ccggatgatc cgccgcatcg
     1321 tcgccgagtc gtctacgatc aatactcgca tcttctgatt tcctccccgt tctggccggt
     1381 tgtcagctta gagcaatatc tatagctcat actccagaac ccggcacacg atagagcaag
     1441 cctggtgcca agtccaaaat aagccgaaaa ccctcattct gacctcaatc agtgtccgac
     1501 agcggcgatt tacgtgtcat taaagggtag ccagggcgct tcaaatggac gacggcggct
     1561 aggatttggc cacagccggt acgacggagc gggaagttgg cgaaagcagg gtaagaatag
     1621 aggcggaata ggtgaggcat aaatttggcc acggtggaaa aaagcttacc accacaaggg
     1681 gcacgtcatt gcgagtaccc catctagcgg tggctgaccc gcaggtggga aaaacttacc
     1741 agtttctgag tagcgactga aaggagcgcg acaatctcac cgttcttgcc ggtccaacac
     1801 catgactcca aataccatga gattgccacg gtcgctccgc tccctcgcaa tgaccactgg
     1861 ggggactttc gaaccaatga gcggatcacc gcagcaatcc gataaagttc taccgacggt
     1921 gtgccgataa tttaggaggg ttacgcagtc cctacgctga ccagactaga aaggagacgc
     1981 gtgctagcga tcatcggagt cgttgtcgtc tttgggtcgg ttatcggcgg ttttgccata
     2041 gagggcggac cgatccccgt tcttatccag ccggtggagt tcctgatcat cggcggcgcc
     2101 tcgattggcg gcctgctgat cgccgtccca ataaaaatcc tcaagatcct catccgacag
     2161 atcatgggca cactcggatc agggctgggt aagcaggcgt acctggaagt ccttcaggtc
     2221 ctgtacgatc tcttcacaaa ggccaagcgg gaaggattca ccgccgtcga ttcggacctt
     2281 atgagtccgg agaagagctc gatcttttct aagtacaagg gatttatcaa gaaccaccac
     2341 gcgatgacct ttctctgtga ctcgctgcgt ctggtggtag acggggcggt cgatgccgac
     2401 gaactcgaag ccatcatgga caccgagctg gagacacatc acgaagaggg cggtcagacc
     2461 ccctcgatca tgagcaaagt cggcgacagt ctgccgggtc tcggtatcgt cgccgctgtt
     2521 ctcggtatcg ttatcacgat
//
LOCUS       DATMXI010000033         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_215746, whole genome shotgun sequence.
ACCESSION   DATMXI010000033 DATMXI010000000
VERSION     DATMXI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215746"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VN444_00655"
     CDS             complement(<1..559)
                     /locus_tag="VN444_00655"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWR20353.1"
                     /translation="MIYLKHVEGSKKGQVESFDSERIRIGRHPDNDLTFDPEAAREVS
                     GHHAEIVCHKDAVTIKDLQSRNGTYVNGRRINQPTRLTDGDVIQFSSRGPKLVFSIGE
                     PVAGSGTVALKHEEIAAPETTAKKAAGIGTRERIALGAAATVILVVLGLAFWWSWPLF
                     FITLGIMVVVISGGLLAWWWKKRQAR"
     gene            990..2048
                     /locus_tag="VN444_00660"
     CDS             990..2048
                     /locus_tag="VN444_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HWR20354.1"
                     /translation="MRMRVAAPNAMTRLNRTFVLSSVVLATLLGAVATSRSAESSNPW
                     ERGEYRDVKPQASAAKASSGVTVRSIGPVLKLERTAVQRPRLSPGDPTSLLAEYSVTG
                     PAGTIDVKETRIIEFNGTVLSKLVKTVPRSNGPAGSEYRLRIPQDAAEGWYTVTTVLE
                     PAKVTTRSIATEQANTAFYIETPQAAEPPSAIERPQKEGESPRVEPDGGIKIKLGSDK
                     PRYKVGDTITMQFETNRDAYLTLVNVGTSGKITILFPNRFSGGHEIKGKQVYTIPAPD
                     DSYELAVSGPPGVELIYALVTSKPVKFVETDFSQTPEIFQTVTGDMPVLTRDINAVVK
                     KTPLKGQAKAVLELEVVP"
     gene            2086..3228
                     /gene="icmH"
                     /locus_tag="VN444_00665"
     CDS             2086..3228
                     /gene="icmH"
                     /locus_tag="VN444_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HWR20355.1"
                     /translation="MSQPDERETQTTLGDLLTDLMAYALIFCQASGGERPPGLVQARG
                     KITELLGEQEKRVKAGETNWESYREARFAVLAWIDELILNQPWASRAQWQHLMLTYYG
                     TLNAGEEFFDHLEKLPSEAKDVREIYYLCLRLGFQGKYAVAGKPDELRQLRYALYRQV
                     TGPSDDIRQRYHRLFPEAYEKPPAEERRARSAMWQLWCGVAILVPAVLFLTYWFILGR
                     ETNRLLASMERQVVVPAPPVVDWGRSLVEELRRKGIEARETPLGVVITLPGLLFEVNR
                     AELGPGAEGKISDVALVVKRHAPTRTVSVEGHASREPGTLDERNFRLSEDRAKRVAGI
                     LVSNGLGPDKVTAKGLGSSKPVTSNDSEEGRRKNRRVELIVEKAAE"
BASE COUNT          766 a          956 c         1001 g          676 t
ORIGIN      
        1 gtctagcctg acgctttttc caccaccagg ccagcaggcc cccagagatg acaacgacca
       61 tgatgccaag ggtgataaag aacagaggcc aggaccacca gaaggcaagg ccgaggacga
      121 ccaggatcac tgtggccgcc gcacccaggg caatgcgttc gcgagtgccg atacccgcag
      181 ccttcttggc ggttgtctcg ggagctgcga tctcctcgtg cttgagcgcc accgtcccgc
      241 ttccggccac aggttcaccg atggagaaaa ccagtttggg gccccgcgaa gagaactgga
      301 tgacgtcgcc gtcagtaagc cgagtaggct ggttgatgcg ccgcccattc acataggttc
      361 cattcctgct ctggagatcc ttgatcgtga cggcgtcttt gtggcagacg atctcggcgt
      421 ggtgaccgga cacctctcga gcggcctcgg ggtcgaatgt caggtcgtta tccggatgcc
      481 gtccgatcct gatccgctca gaatcgaacg actcgacctg ccccttcttg ctgccttcta
      541 cgtgtttgag atagatcatc gactgatcat gtcaagtcgg tatccatttt aatacagcaa
      601 tgggaaatcg cggtcagtca aaggtgaaca tattagatta aactcggcga acagccaaag
      661 aatcccgctc agtccacgta tggcatttaa tatgctatcc cggaaaggaa gtgtcaagct
      721 ccaaaaacag cgatagaaac ttgccagtgc gaacgaagtg aagcaatctc gcattattgc
      781 actgagcttg ccgcgctgac tttcccagga attgcactct aaggtcgacg ttgggatgag
      841 gatacacgag aaagtcagta caatccagtg tccagataca gaaggtctgg ctgcatctct
      901 tgacatcagc acgacctgcg cttacacttc gttcattctc tggagctgga tgcccgcgtg
      961 gtggatatca catcgccagg aggaacaaaa tgcgcatgag agtcgcagcc ccgaacgcaa
     1021 tgacccggct caaccggacc ttcgttctat cctcagttgt gcttgccacg cttctgggag
     1081 cggttgcgac aagccgctcc gcagaatcat ccaatccctg ggagaggggt gaatatcgcg
     1141 atgtcaagcc tcaagcctcg gccgctaaag cctcctctgg cgtgaccgtc cgctcaatcg
     1201 ggccagtgct caagctcgaa cgcacggccg tacagcgccc caggctgagc cctggtgatc
     1261 ctacttccct cttggccgaa tatagcgtga ccggcccggc cgggaccata gacgtgaagg
     1321 aaacccgcat catcgaattt aatggaacag tactgagcaa actggtcaaa actgtgcctc
     1381 gatcaaacgg gcctgctgga agcgaatatc gactgagaat cccacaagat gcggctgagg
     1441 ggtggtacac ggtgacgacg gtcctggaac ccgcaaaggt gaccacgcgc tcgattgcga
     1501 cagagcaggc caataccgcc ttttacatcg agacgccaca ggccgccgag ccgccctctg
     1561 ctatcgagcg cccgcagaag gaaggcgaaa gtccgcgcgt tgaacccgac ggtggcatca
     1621 agatcaaact cggatccgat aagcctcgat ataaggtggg cgatacgatc acgatgcagt
     1681 tcgagacgaa ccgagacgcg tacctcaccc tcgtcaacgt ggggacgagc ggcaaaatca
     1741 cgatcctgtt tccgaaccgg ttctccggcg gccacgagat caaaggcaaa caggtctaca
     1801 ccattcctgc gccggatgac agctacgagc tcgcagtgag tgggccgccg ggcgttgagc
     1861 tgatctatgc gttggtcacg tcgaaacctg tcaagttcgt cgaaaccgac ttctcccaga
     1921 caccggagat cttccaaacg gtaaccgggg acatgcctgt cctcacccgc gacatcaatg
     1981 ccgtggttaa gaagaccccg ctcaaggggc aggcaaaggc cgtgttggag ctggaggttg
     2041 tgccgtaata aaatgggtgt tgcggtgcgt gagaaggggt cgaatttgag ccagcctgac
     2101 gaacgagaga ctcagaccac actgggagat ctcctcaccg atctgatggc gtatgcatta
     2161 atattctgcc aagcctccgg cggcgagcgc ccccccgggt tagtccaagc gcgcggaaag
     2221 atcacggagc ttctgggcga acaggagaaa cgggtcaagg ccggggagac gaattgggaa
     2281 tcgtaccgcg aggcaaggtt tgcagtcctg gcttggatcg acgagttgat cctgaaccaa
     2341 ccctgggcct ctcgcgccca gtggcaacac ctcatgctca catactatgg cactctgaat
     2401 gccggggaag aatttttcga ccatctggag aagctgccgt cagaggctaa agatgtcaga
     2461 gagatttact acctctgctt gagactgggt ttccagggga aatatgcggt agcgggaaag
     2521 cctgatgagc tccgccagct ccggtatgcc ctttaccggc aggtcaccgg cccttctgac
     2581 gatatccgtc agaggtacca caggctgttt cccgaggcat acgagaagcc accggcagag
     2641 gaacgacgtg cccgctccgc aatgtggcaa ctgtggtgtg gtgtggccat ccttgtgccg
     2701 gccgtgcttt ttctcaccta ttggttcatt ctggggcgcg aaaccaaccg cctgctcgct
     2761 tctatggaac ggcaggtcgt ggttccagcg ccaccggtcg tcgactgggg tcgatctttg
     2821 gtcgaagagc tcaggcgaaa gggaatcgag gcgcgcgaga cgccactagg ggtcgtgatt
     2881 acgcttccag gtctgctctt cgaggtcaat cgagctgagc ttggtcctgg tgctgaagga
     2941 aagatctcgg atgtggcgct tgtcgtgaag cgtcatgctc ctacgcggac tgtgtcggtg
     3001 gaaggccatg cgagtcgaga gccgggaaca ttggacgagc gcaactttcg actttctgag
     3061 gatcgagcaa agcgggttgc tgggattctg gtaagcaatg gtctcggccc tgataaggtc
     3121 acggcgaagg gactgggttc cagcaagccg gtgacctcca acgattcgga agagggacga
     3181 cggaagaacc gccgggtgga acttatcgtc gaaaaggcgg cagagtgata cgcgcgcttg
     3241 ccgtcacctg gcggctctac agaagactgc acacctgagg cttgattgag gagatcgtct
     3301 cctccagatt gccgcgctgt tctctttgcg gccgcatctg gcctgctgct ggctgtggca
     3361 gcctgtagca cgccggctca gagattctct gaacgtgcc
//
LOCUS       DATMXI010000034         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_215675, whole genome shotgun sequence.
ACCESSION   DATMXI010000034 DATMXI010000000
VERSION     DATMXI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215675"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VN444_00670"
     CDS             complement(<1..507)
                     /locus_tag="VN444_00670"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWR20356.1"
                     /translation="MTGIVLAGGKSSRMGSNKAYIEFGGKRLIEATVDCLRALFPEVL
                     IIANDPPLYAYLGVKVISDLIPDSGSLGGIYTGLSAASYPTCFFVACDMPFLNADLIK
                     LLVREAEGWDVVVPRVGGELQPLHAVYARSCLPLMKEAIDTGVLKITRFFPKAKVNII
                     EESILREVD"
     gene            complement(510..950)
                     /gene="rpoZ"
                     /locus_tag="VN444_00675"
     CDS             complement(510..950)
                     /gene="rpoZ"
                     /locus_tag="VN444_00675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWR20357.1"
                     /translation="MPLFPLEQLLTHVDSKYRLVIIAAKRAKQLMHGAEYLIAPKSSK
                     PPYIALEEIGAGKIAYDMKPVEGTGAVELVGPEAGATWFRSLSVGDTLGEEEIVEKEE
                     EEKDGAELEETPAELLAESGEEIEKLEVTDLDVMEEPAEVEDEA"
     gene            complement(970..1578)
                     /gene="gmk"
                     /locus_tag="VN444_00680"
     CDS             complement(970..1578)
                     /gene="gmk"
                     /locus_tag="VN444_00680"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWR20358.1"
                     /translation="MNRQQMMVVVSAPSGAGKTSLCQEAARRLPRLIHSVSYTTRAPR
                     PDELDGRDYNFVNESTFRMMIEADEFAEWAYVHGHLYGTSRPLLEKQFAEGLDVILDI
                     DTQGAAKLRQGYRTGVFVFVVPPTFDLLETRLRQRRTDSEEEIFRRLAMAREELHHYR
                     HYQYIVVNDIFEKAVKQLCCIITAERSRRDRVDLSFLDMGID"
     gene            complement(1575..1856)
                     /locus_tag="VN444_00685"
     CDS             complement(1575..1856)
                     /locus_tag="VN444_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HWR20359.1"
                     /translation="MATKLLNVGFGNMVAVVRVIAIVDPGSAPMKRLKDEAKQAGKLV
                     DATNGRRTRSIVVTDSDHIILSAIQTETLAQRFEADALSDRLGKGSRAE"
     gene            complement(1895..2764)
                     /locus_tag="VN444_00690"
     CDS             complement(1895..2764)
                     /locus_tag="VN444_00690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWR20360.1"
                     /translation="MTGFGQGECVTSSRRYACELQSVNHRYLETRTKLPKRLGTLELQ
                     VQKILQGRFARGRFGVTVLEELTGERSCKLRLNRPLAHAYLDAVKTLQSELGLAGEVT
                     LELLLSQSDLFGLEGEEPGAADADWSAVKTALEGAMNALAEMRREEGWALKADLLDHL
                     EQVEAILATIAARAPEVVQNYKSRLELRLQRLLEGKPVDPGRLEQEVAILADRSDIAE
                     ETARVTSHLRQFRDLIQQQGPHGRRMEFLLQEMQREANTIGAKANDAKTSHDVIALKS
                     ILEQLREQVQNVE"
BASE COUNT          571 a          929 c          776 g          635 t
ORIGIN      
        1 gtccacctcc ctgagtatcg actcctcgat gatgttcacc ttggccttcg ggaagaatcg
       61 ggtaatcttc agtacgccgg tatcaatggc ctctttcatg agcgggaggc acgacctggc
      121 gtatacggca tgcagcggct gtaactcgcc ccctacacgt gggacgacca catcccaccc
      181 ttcggcttcg cggaccaaga gcttgatcag gtcggcgtta agaaaaggca tatcgcaggc
      241 aacaaagaaa cacgtggggt aacttgcagc actgagtccc gtataaatcc caccgagcga
      301 gccggagtcc gggatcagat cagagatgac cttgacaccg aggtacgcat agagcggagg
      361 gtcattggcg ataatcagga cctctgggaa gagcgccctc aggcagtcca ccgtcgcctc
      421 gatcagccgc ttgcccccga attcgatata ggccttattg gatcccatcc gcgaggactt
      481 gccgcccgcc agcacaatac cagtcacctt taggcttcgt cctctacttc cgctggctct
      541 tccatgacat cgagatcggt cacctctaat ttctctatct cctcgcccga ttcggcgagc
      601 agctccgccg gggtctcctc caactcagct ccatcctttt cctcttcttc cttctcgacg
      661 atctcctcct cgccaagggt gtccccaacg gagaggcttc ggaaccaggt agccccagcc
      721 tctggaccaa ccagctccac tgcccccgta ccctctacgg gcttcatgtc ataggctatc
      781 tttccggcgc ctatttcctc cagggcgata tacggcggct tactgctctt cggggcgatc
      841 aggtactcgg caccgtgcat caactgcttc gcgcgcttgg cggcgatgat caccaagcga
      901 tacttactat ccacgtgcgt cagaagttgt tccaaaggga aaagcggcat gtctaagatc
      961 ctccctacct tagtcgattc ccatatccag aaaggagaga tccactcgat ctcttcggga
     1021 tcgctcggca gtgataatgc agcaaagctg cttgacggcc ttttcgaaga tatcgttcac
     1081 gacaatatac tggtaatgcc ggtagtgatg cagctcctct ctggccatag ccaggcgccg
     1141 gaagatctcc tcctccgaat cagtccgccg ctgccgaagc ctggtttcca gcaggtcgaa
     1201 ggtgggggga acgacaaaca caaacacccc cgttcggtag ccctgcctga gcttggctgc
     1261 cccctgcgtg tcgatgtcaa ggatgacatc aaggccttcc gcaaactgtt tctccaacag
     1321 cgggcggctg gttccataga ggtgaccatg cacgtacgcc cactctgcga attcgtccgc
     1381 ctcgatcatc attcggaagg tggactcgtt cacgaaatta tagtcgcgtc cgtccagctc
     1441 gtcgggccgg ggcgcacgcg tcgtataaga gacagagtga atcagccggg gcaaccgccg
     1501 tgcggcctcc tggcacaagg aggtcttccc agccccggag ggggccgaca cgaccaccat
     1561 catctgctgc cgattcattc tgctcgcgaa cccttaccca gacgatcgga aagcgcatcg
     1621 gcctcgaatc gctgggctag cgtctcggtc tggatggccg acagtatgat atggtcacta
     1681 tcggtcacga cgatagaccg ggtacgcctg ccgttggttg cgtcaaccag tttaccggcc
     1741 tgcttggcct cgtccttcaa ccgcttcatt ggggccgaac cgggatcgac aatggcgatg
     1801 accctgacga ccgccaccat atttccgaac cctacgttca gtagctttgt ggccaaggcg
     1861 gtttatccct ttccccgatg tctcgcggac aggcttactc gacgttctgg acctgctccc
     1921 ggagctgctc cagaatactt ttcaatgcta tcacatcgtg cgaggtcttg gcatcgttcg
     1981 ccttggcgcc gatggtgttg gcctcgcgct gcatctcttg caagaggaac tccatccgtc
     2041 ggccgtgcgg accctgttgt tggatgagat cccggaattg tcggagatgg cttgtgaccc
     2101 gcgcggtctc ttccgcgata tccgaacgat cagccaggat tgcgacctcc tgttccagcc
     2161 ggccgggatc gaccggtttc ccctccagca agcgctgaag ccgaagctcc agacgactct
     2221 tgtagttttg caccacttcc ggcgcgcggg cggcgatcgt cgccaagatc gcctcgactt
     2281 gctccaggtg gtcgagcagg tcggccttaa gcgcccatcc ctcttcccgc cgcatctcgg
     2341 cgagggcgtt cattgcccct tcaagggcag tcttgaccgc tgaccaatca gcatccgccg
     2401 cccccggctc ctctccttcc aggccgaaca ggtctgattg ggaaagtagt agttccagcg
     2461 tcacctctcc cgcgagaccg agttccgact gaagcgtctt gacggcgtca agatacgcgt
     2521 gggccagcgg acgattgaga cgtagcttgc aggaccgctc acccgtcaac tcctcaagca
     2581 ccgtcacgcc aaagcgccct cgcgcgaagc gtccctgcag gatcttctgg acctgcaatt
     2641 ccagggtgcc aagccgcttc ggaagcttgg tgcgggtctc caggtaacga tggttgaccg
     2701 actgcagttc acatgcgtat cgtctcgagg aggtgacaca ctccccttgc ccgaacccgg
     2761 tcatactgtt cagcatctcg tcacggcctc gataatgttg gcatgagtgt tgccactacc
     2821 tggataggtg gacgcgaagg gggtcgcgcg gggatcttct gtggcattaa tctagccata
     2881 atcggcagtt ctgtcaaatc aaattctcaa c
//
LOCUS       DATMXI010000035         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_278619, whole genome shotgun sequence.
ACCESSION   DATMXI010000035 DATMXI010000000
VERSION     DATMXI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278619"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VN444_00695"
     CDS             <1..898
                     /locus_tag="VN444_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR20361.1"
                     /translation="NQPVMARATALAMLPEYLSAASFPAVETAIGDVDPLVRSTALAA
                     IETLPPTEQVRLAAPKLRDPVRAVRLAAAHALAGVPRQTLSAGQQANFDSALAELIAS
                     ETVNADRPEAHLNLANLYARLGRAVDAEAELRTALALDPRFVPALVNLADLLRAQGRE
                     ADGERALEQALRVVPDSDEALHALGLLRVRQGRRAEAVALLRRVTQRRPESTRFAYVY
                     AVALYGTGDPGGAIAVLEAAHQRRPANRDVLTALASYLRERGDSRGALRYAERLATLT
                     PSDREVRGLVESLRRQAGGTSP"
     gene            complement(1176..1580)
                     /locus_tag="VN444_00700"
     CDS             complement(1176..1580)
                     /locus_tag="VN444_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWR20362.1"
                     /translation="MCKPVVPPFGKGGQGGFLMILPYNKDLKGCARSLRKNMTDAERT
                     LWSMIRRKQAKGRLFSRQKTIDHYIVDFYCASAALVIELDGGQHYTPEGREQDQRRDD
                     DLAARGLTVLRFSDRDVLTNLEGVQEEIWKHL"
     gene            complement(1948..2076)
                     /locus_tag="VN444_00705"
     CDS             complement(1948..2076)
                     /locus_tag="VN444_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20363.1"
                     /translation="MKYIQRQQADRLRFLLRSFPAVLLVGPRQCGKSTLADQLDLF"
BASE COUNT          463 a          727 c          703 g          463 t
ORIGIN      
        1 gaaccagccc gtcatggcca gggcgacggc actcgcgatg ctccccgagt acctgagtgc
       61 agcatcgttc ccagcggtcg agacggcgat aggtgatgtc gatcctctgg tgcggtccac
      121 ggcgctggct gcgatcgaga cgctcccgcc gacggagcaa gtgcggcttg ccgcccccaa
      181 actccgcgat cccgtgcggg cggtacgcct ggcggcagcc cacgcgctgg ccggagtccc
      241 gcggcagacg ctctctgccg ggcaacaggc caacttcgat tcggccctcg cagagctcat
      301 cgcctcggag acggtcaacg ccgaccgtcc ggaggcgcac ctgaatctgg ccaatctcta
      361 tgcgcgactc ggccgggcgg tcgatgccga ggccgagctg cgcaccgcgc tcgctctcga
      421 cccgcgcttt gtcccggccc tggtcaacct ggccgatctt cttcgggcgc aggggcgcga
      481 ggccgacggc gagcgcgccc tggaacaagc gctccgggtt gtccccgaca gcgacgaagc
      541 gctccatgcg cttggcctgc ttcgggtgcg acaaggtcga cgtgcagagg cggtggctct
      601 cctgcgccgg gtgacgcagc gccggccgga gtccacgcgg ttcgcgtacg tctatgcggt
      661 tgccctctat gggacgggcg acccgggcgg ggctattgcg gtgctcgagg cagctcacca
      721 gcgacgaccg gccaaccggg acgtcctgac cgcactagcg agctatctcc gagaacgcgg
      781 ggacagcagg ggcgcgctcc gctacgccga acggctcgcc acattgacgc ccagtgaccg
      841 tgaggtacgg ggtctggtag agtcgctgcg tcgccaggcc ggcggaactt ccccttgacc
      901 taccgacggg caacatcctt atttttcggc tcttagcagg aacgtgcagg gagatccgtc
      961 cccccgtatc aattcagcca ttgccttacc gttgccggca ttcgacacct tagctcagtt
     1021 gcggagttta tctcacaagc ctctcaagta tttcggttct tccggaatta gcgtgggtga
     1081 acctggaggc aggtaggaat cagaactccc cctcccctta ctttctttct ccccctttag
     1141 caaaggggga ttgagggggt tttcgcctct ttccctcaca gatgtttcca gatctcttct
     1201 tgcacgcctt caagatttgt caggacgtcc cgatcagaaa acctcaacac ggttaaccca
     1261 cgcgccgcca gatcatcatc gcgccgctgg tcctgctcac gaccctccgg cgtataatgt
     1321 tgtcctccat caagttcgat gacgagcgcg gctgaggcgc agtaaaaatc cacaatgtaa
     1381 tggtcaatcg tcttttgacg tgaaaagaga cggcctttcg cttgctttcg cctgatcatg
     1441 gaccacagtg ttcgctcggc atcggtcatg ttcttccgca gggagcgggc acaacctttg
     1501 aggtccttat tgtagggcag gatcatgaga aacccccctt gccccccttt tccaaagggg
     1561 gggaccacag gcttgcacat ccacctcttc tcccccttta gcaaaggggg attgagggga
     1621 gtttctctcc ctccccatgg cctcgtttgg caaagggggg tgtccccatg tccagcgctg
     1681 aagactcgct ctacccacgc tgattccgga agaaccaata tttcagcatc catgaatatt
     1741 cggaatgtca atccgaatct gcacggatca atattactcg tgctacatga tcggcacgcg
     1801 gagcaaggga acgagacctc gctgaagagg acagtgagtc gagccaaggc gatcgcggtg
     1861 tttgagaagt ctcaccaccg gcgtcaccaa tcgtctcgac tgtcgcgccg cgcgcaaccg
     1921 ccggattgag aaggctgaga aataacctca gaacagatcg agttggtcgg cgagcgtgct
     1981 cttcccgcac tggcgcgggc ccacgaggag tacggccggg aaagaccgaa ggaggaaccg
     2041 aagacgatcg gcctgttgtc gctggatata tttcatacca tgtagtattg cactgtagat
     2101 gcaaaaatac aagatataca aatcgagtag cagccggata caatacgcct gcatgatgcg
     2161 aagaaaacga tgagtcgagc tagcctgccg acgggcgtca acacccttgt gtttcggctc
     2221 ttggcggaaa agtgtaggga gattccttcc ccccatgagg gggaagggtg ggatgggggg
     2281 tgaataggaa agccggaacg ctgaggaagc gctcaacctg catgtccggc cactgcccgc
     2341 tcggcgtgtg cgcgtt
//
LOCUS       DATMXI010000036         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_83051, whole genome shotgun sequence.
ACCESSION   DATMXI010000036 DATMXI010000000
VERSION     DATMXI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83051"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1747)
                     /locus_tag="VN444_00710"
     CDS             complement(74..1747)
                     /locus_tag="VN444_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20364.1"
                     /translation="MKDMGTCSECGIAIGPGHRKQHVLAGYQLPQICNEMLYISEAGK
                     ETKAEWLEEGIVLKISDAVRTRTGYEANLKVKHRSEKKDVWRVSDRLNFHKSRARKVL
                     ADQIFEAFQGDNGTLTHDDIGQLLLWVEQYLESHYGGQGQKALDLPDEKDHLTPEEHE
                     AAMALLKSPDLIDQFLAAVDTLGCVGEEVNKTYFLLAFTSRLLPEPIHSILKGEPSAG
                     KNHLLFSVGKLFPEGEIKFLTGASAKALFYSGDDFRHKCLIFIEHVGGEESDYSIRTM
                     QSEQKIVFWTVEKVDGRLETVEKVVEGPVGITQASTAAHLNAENETRCFTPNMDETDD
                     QTRRILRSKATNYKLNGKRSHDDIIKQWQTAMGLLSQVKVVFPEWLDHVVESFPVKPV
                     RVRRDFGRFVALIEASAVLHQHQREVTEFEEGVKIVTPSIEDYRLAYEIVDASSLLDI
                     AVSGITPKCETLMAAIRAWDDARVQVGDNSFSVSDVAHLVDWTLNTVRKHLREAVHVG
                     ALELTTPGGKGRGHIARYKLLSASRVPGRFTMPISPDLLFPHTVIQKDT"
BASE COUNT          632 a          842 c          706 g          632 t
ORIGIN      
        1 gttacataaa taattcaaaa atgtcaaagt gccttgacat tttgggacaa caatgtccca
       61 cttttagtgc taattatgta tccttttgga taacagtatg aggaaacagc aaatcagggg
      121 aaattggcat ggtaaatcga cctggcacgc gagatgcaga gagcagttta tatcgtgcga
      181 tatgtcctcg tccctttccc ccaggagtcg tcaattcgag cgcccccaca tgcacagctt
      241 cacggagatg tttgcgaacc gtgttgagcg tccaatcgac gaggtgggct acgtctgaca
      301 cgctaaacga attgtctccg acctggactc gcgcatcatc ccatgctcgt atcgccgcca
      361 tcaaagtttc acatttcggc gtaatgccgc tgacggcaat atctaacaag gatgacgcat
      421 caacaatctc atacgcgagg cggtagtcct cgatagacgg tgtgacaatc ttcaccccct
      481 cttcgaattc ggtcacctcg cgctgatgtt ggtgcagcac cgccgaggct tcaataaggg
      541 cgacgaaccg gccaaaatct ctccgcacac gaaccggctt gaccgggaac gattcaacga
      601 catggtcgag ccattcaggg aagacgacct tcacttgcga gagaaggccc atcgcggttt
      661 gccactgctt gataatgtcg tcatggctac gctttccatt gagcttataa ttcgtcgcct
      721 tcgaccgcaa gatgcgccga gtctgatcgt cggtctcatc catgttcggc gtaaaacagc
      781 gcgtttcgtt ttcagcgttc aaatgtgccg ccgtgctcgc ctgagtgatc ccaactggcc
      841 cctcaacgac cttctcgacc gtttcgagcc gaccatcgac cttctcaacg gtccaaaaca
      901 caatcttctg ctcggattgc atggtgcgga ttgaataatc gctctcctcg cctccgacat
      961 gctcgataaa aatcaagcat ttatgccgga agtcgtcgcc gctgtagaat agggccttcg
     1021 cggaggctcc cgtcaagaac ttaatctcac cctcagggaa gagcttgccg acactaaaca
     1081 acagatggtt cttcccggcg gacggctccc ccttcaaaat cgagtgaatc ggttcgggca
     1141 ggaggcgtga cgtgaatgcc aacagaaaat acgtcttatt gacctcctcc ccgacgcacc
     1201 ccaacgtatc aaccgccgca agaaactggt caataagatc aggcgacttt aacaatgcca
     1261 ttgccgcttc gtgttcttct ggggtgagat gatccttctc gtccggtaga tcgagcgcct
     1321 tttgcccctg accgccgtag tgcgattcga ggtattgttc cacccacaaa agcagttgcc
     1381 cgatgtcgtc atgcgtcaat gtcccattgt ccccctgaaa cgcctcgaaa atctggtcgg
     1441 ccaggacctt ccgtgcccgc gatttgtgaa agttcagccg atccgagacc cgccacacat
     1501 cctttttctc gctgcgatgc ttaaccttca ggttcgcctc ataccccgtt cgcgtcctca
     1561 ccgcgtcgct gatcttcaga acgatcccct cttccagcca ctccgctttc gtttccttgc
     1621 ccgcctccga gatgtatagc atctcgttgc agatttgcgg cagttgatac ccggccagga
     1681 cgtgttgttt gcgatggccg gggccgatag caatcccgca ttcggagcat gtgcccatat
     1741 ctttcatcaa gtctctccag ggttgaaagt tgcgccagac taccgcctct ttttatccaa
     1801 tcactgaaat cgttgtcgcc cttctcgccg ctcaacggca gccgtaacac cgtcgcggaa
     1861 atctgcgcct tcacgaggaa ttccctgacc cgcaacgcgc catcaatgcc tggcttgtcg
     1921 cagtcgtaga ggatcgtcac ggttttacct gctagttgtg ccgtccattc cggtttccag
     1981 caattcgacc ccccacttgt acacccgacc aggggcgccc ccaatgaata ggccagcagg
     2041 gcgtcccatt ccccctcgca gagcaacagc gtggccaaat tgacatctcc ccaatggaga
     2101 agctgtgcct tcgctccgtc agtgtgtagc cacttgtgtt cttttgcaag ccgaacatgc
     2161 catccacccc caggcagggc aaacccgacc ccggagtcta caacgcgccc ctgacgccac
     2221 acacgccccg gaaagatcga gaggctggag agtaggtccg ggttggcaac cttgccgtgt
     2281 tgggtcaacc atgccagaga cagagggcac cattgacgct gtgtgaacgt cagcatcggc
     2341 ttcggagtcg gattgagtgc agggacagat tcttttcgtg gtgttagggg ccgattctca
     2401 ccaagcgccc ggagaaagta gtcgatgcct cctcgaaaat tacagccaaa gcacacaacc
     2461 gcgttctcat agacgtgtag gcttggcttg gaatcgtcgt gaaatggaca atgccaccac
     2521 ccgcgcctcg atggtccgag ccgggaggcg acaatctcag cggcagagtc agacatcatc
     2581 ggtcaaccgg cacgacggcc aaagaagggg aaaagacagg tggtgaagcg tccatcgagt
     2641 gtgccctccc acgggctccc acggaaagga agaaacagca ggccgggtgg gaagcccggc
     2701 ttttcggata cgtggtccta gctgtttccg gcaagcagac tacgttctct tcgtatcacg
     2761 ccagccgaga tgtgtcaaga ttcggatcgg gagtcgttat tggaaccaga tc
//
LOCUS       DATMXI010000037         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_82602, whole genome shotgun sequence.
ACCESSION   DATMXI010000037 DATMXI010000000
VERSION     DATMXI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82602"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VN444_00715"
     CDS             <1..957
                     /locus_tag="VN444_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20365.1"
                     /translation="VATQGDLLAGVVVLLAIAWGGGLLFGIHRWLVKPLRAIGRSTTI
                     ISTGELDHHIQVASQDELGDLAASINRMAQSLKGIQERLLQAERLAAAGELSSYIAHN
                     IRNPLASIRSAAQAALKGPEQSAEVRLTLQSIVESVDRLNQWVYNFLFALKPITPTLI
                     PFDLDRVVIQAVDVVRPMIEGKQITLDLSPSPTLPLVPLDEGYLEQALVALLANACEA
                     TPRKGNIVITSRLIHEGEGPGGVAIEFADNGEGIPPDILQKVFTPYFTTKSDGVGLGL
                     TMARKFISAHGGSLTVSNSPEGGTTVQVLLPITYPTGADRNG"
     gene            950..2368
                     /locus_tag="VN444_00720"
     CDS             950..2368
                     /locus_tag="VN444_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR20366.1"
                     /translation="MAKILVIDDEVVFARSVAHTLSRHGHECRSLPSAEEGMTSLEAD
                     RPDLILLDIKLAGMSGLDALRRILSLDGSIIVIIMTAYSSVESAVAAMKDGAYDYIQK
                     PIDHDELTFIIEKALETHRLRERLSYFQRKEIQQAAHGEIIGVSAGMTHVIEMIGRIA
                     QLEPRPAGELPTVLLLGETGTGKDLVARAIHRRGSLAGQPFVEIECTSIPKDLAEAEL
                     FGYEKGAFTGATAAKPGLIEAADGGTLFLDEIGELSPDLQAKLLKAIERRQIRRLGSL
                     RERKINVRIIAATNRDLEAAVGAGTFRQDLYYRLKVLIVELPPLRERGADVVLLANHF
                     LRQLTHKYSLPVRRFTEAALHALKKYRWPGNVRELAHLVERALLVCDEQEIGPSALGM
                     DATFPVVASQRRNHPPGADETFQIQCPEQGIDLEEVERQLIKQALDLTDGNVAEAARK
                     LNIGREALRYRIQKHGIVRPAS"
BASE COUNT          541 a          672 c          762 g          515 t
ORIGIN      
        1 gtcgcgactc agggagatct gctggccggg gtcgtcgtac tgctcgccat cgcgtggggc
       61 ggcgggttgc tgttcggaat tcaccgatgg ctggtaaagc ctcttcgggc gatcggtcgc
      121 tcgaccacga tcatcagtac cggggagttg gatcatcata tccaggtcgc gtcccaggat
      181 gaattgggcg atctggcagc ctcgatcaat cgcatggccc agtcgctgaa ggggattcag
      241 gaacgcttgc ttcaagccga acgattggcg gcggccggcg aactctcttc ctatattgcc
      301 cacaatatcc gtaacccatt ggcgagtatc cggtctgccg ctcaggcagc cctcaaagga
      361 ccggagcagt ccgcagaggt gcgactcacg cttcagagca ttgtggagag cgtcgatcgc
      421 ctgaaccaat gggtgtataa ttttctcttt gccttgaaac cgatcacgcc gaccttgatc
      481 cctttcgatt tggaccgagt ggtcatccag gcggttgatg tggttcgtcc gatgatcgag
      541 gggaagcaga tcacgcttga cttaagtcct tccccaacac taccgcttgt tccccttgat
      601 gaggggtatc tggaacaggc cctcgttgcc ctgctcgcta acgcgtgcga agcgaccccc
      661 agaaagggga acattgtgat cacctcccgg ctgatccacg aaggtgaggg gccaggcggc
      721 gttgctattg agttcgctga caatggagag ggaatccccc ccgacatact ccagaaggtg
      781 tttaccccgt actttactac gaagagcgat ggcgtgggcc ttggcctgac aatggcccgg
      841 aagtttatca gcgctcacgg gggaagcctc accgtgtcga acagcccaga gggtgggact
      901 acagtacagg ttctgcttcc gatcacctat cctacaggag ccgatcgcaa tggctaagat
      961 actggtcatt gatgacgagg tcgtgttcgc tcgctccgtg gcgcacaccc tcagcagaca
     1021 tggccatgag tgcaggagtc tgccgagcgc agaggagggg atgacatcgc tcgaggcgga
     1081 tcggcccgat ctgatcctgc tggatatcaa gctggccggt atgagtggac tcgacgcgct
     1141 gaggaggatc ttgagcctcg acgggagcat catcgtcatc atcatgactg cgtatagcag
     1201 cgtcgagagc gctgtggccg cgatgaagga tggggcatac gactacatcc agaagccgat
     1261 cgatcacgac gagctgacat ttatcatcga gaaagcattg gagacgcatc gcctgagaga
     1321 acgcctctcg tacttccaac gtaaggagat ccagcaggcc gctcatggcg agatcatcgg
     1381 cgtctctgcc gggatgacgc atgttatcga gatgatcgga cggatcgcgc agctcgaacc
     1441 cagacctgcg ggcgagctgc cgactgtgtt gctgctgggt gaaaccggga cggggaagga
     1501 cctcgtggca agggcaatcc atcgccgagg aagcctcgcc gggcagccgt tcgtcgagat
     1561 tgagtgtaca tcaatcccga aggatctcgc agaagcggag ctgttcgggt atgagaaagg
     1621 cgccttcacg ggcgccactg ctgcgaagcc tggcctgatc gaggctgccg atggcgggac
     1681 gctctttctg gacgagatcg gcgagctgag tccagatctt caggcgaaac tactgaaggc
     1741 gatcgagcga aggcagattc gacgcctggg aagtctgagg gaacgcaaga ttaacgtccg
     1801 gattattgcg gccactaacc gcgaccttga ggcggcagtc ggagccggca cctttcgcca
     1861 ggacctgtac tatcgtctaa aggtcctgat agtagaattg ccgcctctgc gggaacgcgg
     1921 cgctgatgtg gtcttgctgg cgaaccactt tctcaggcaa ctgacccata agtattcgct
     1981 tcctgtaaga cggttcacag aggccgccct tcacgcgctg aaaaagtacc gctggcctgg
     2041 aaacgttcgt gagctggcgc acttggtcga gcgggcgctt ttggtctgcg atgagcaaga
     2101 gatcggtccc tcggctcttg gcatggacgc aacgttccct gtcgttgcaa gccaaaggcg
     2161 aaatcaccca cccggcgccg atgagacgtt tcagatacag tgtccagaac agggtatcga
     2221 tttggaggag gttgagcgac aattgatcaa gcaggcgcta gacctgacgg acggcaatgt
     2281 cgcggaagcc gccagaaaat tgaacatcgg tagagaagcg ctccgttatc gcatccagaa
     2341 acatgggatc gtccgccccg cgtcataatg agagagcgga ggtttgcttc gtgaatgttt
     2401 gcccttgacc aagaaagaaa ctacgcgggt cttgaagcct cagattattt ttagaccaat
     2461 ctttacagcg gttttggtga gagtcttgtc
//
LOCUS       DATMXI010000038         6306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_214849, whole genome shotgun sequence.
ACCESSION   DATMXI010000038 DATMXI010000000
VERSION     DATMXI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214849"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..326)
                     /locus_tag="VN444_00725"
     CDS             complement(24..326)
                     /locus_tag="VN444_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006529579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HWR20367.1"
                     /translation="MKKSKSSISQARSYTDIGEFWDKHDLSDVWGKTRGVGFDVDIKS
                     EVTYYPIEQNLSEKIQLLARKQGLSSGTLVNLWLEQKMREQGSSSRTARKLTAKNA"
     gene            complement(443..2089)
                     /locus_tag="VN444_00730"
     CDS             complement(443..2089)
                     /locus_tag="VN444_00730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWR20368.1"
                     /translation="MAAKPKLELTWIGKENRPKLEPRILLEDPERSYHAPHRVTDHDL
                     FDNQLIFGDNLLALKALEQEFAGKIKCIYIDPPYNTGSAFSHYDDGLEHSLWLSLVRD
                     RVELLRHLLAQDGSIWITLDDNEVHYMKVLCDEVFGRQNFIANVVWQKKFSPQANAIW
                     LSDSHDHILVYAKDKNTWRPNLLRRTAESDARYSNPDNDLRGSWTSGDFTISLTGGQR
                     GAQFARTGISLNIYEITTPSGRKLMPPRGRCWGTSPDRFKELVADKRIWFGASGNNVP
                     RIKRFLSEVQEGIVCMTIWLRNEVGDNQDAKREVAAFNAEAIFATPKPEKLLQRVLEL
                     ATDPGDWVLDSFAGSGTTGAVAHKMGRRWIMVELGEHCHTHIIPRLKKVIDGEDKGGI
                     TEAVGWKGGGGFRYYRLAPSLLEKDKWDNWVINKAYNSAMLAEAVCKLEGFTYAPSET
                     VYWQHGHSTEQDFIYVTTQNLSHDQLQALSDEVGEHRSLLVLCSAFRGKPDRFPNLTI
                     KKIPKAVIVRCEWGKDDYSLRVENLPKAPPPPGQMELISP"
     gene            complement(2100..2327)
                     /locus_tag="VN444_00735"
     CDS             complement(2100..2327)
                     /locus_tag="VN444_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWR20369.1"
                     /translation="MMCVICKNGTTQLGKATITLEREGTILVIKGVPAQVCANCGEEY
                     VDEATTSLLLKTAEEVVQTGVQVDVRQYAAA"
     gene            complement(2324..2611)
                     /locus_tag="VN444_00740"
     CDS             complement(2324..2611)
                     /locus_tag="VN444_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HWR20370.1"
                     /translation="MNVGPLVFRVHAIKRMFQRRISKNDVRHVLETGETIETYPDDTP
                     YPSRLILGWWNARPLHVVAAENAETRETIVITAYEPDPEEWERGFKRRKRR"
     gene            complement(2835..3167)
                     /locus_tag="VN444_00745"
     CDS             complement(2835..3167)
                     /locus_tag="VN444_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWR20371.1"
                     /translation="MSKSRKSATTDAVEILHRRYYEGRPERLVALEEARANDSVAQKI
                     VVLRVKAGLTQRQLAKLVGTTASVICRLENADYEGHSLAMLNRIAAALNQRVEIRFVQ
                     AREQVQHA"
     gene            complement(3303..6155)
                     /locus_tag="VN444_00750"
     CDS             complement(3303..6155)
                     /locus_tag="VN444_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HWR20372.1"
                     /translation="MNLTDYHAKYFAYELTKRCPSDSLEKLAGALVDAQVDLNPHQIE
                     AALFAFRSPFSKGAILADEVGLGKTIEAGLVLAQKWAERKRRVLVITPSNLRKQWHQE
                     LQEKFFLPCRILETKSYNSAVRQGHVRPFESSETIICSYQFARSKAAEISLMHWDLVV
                     IDEAHRLRNVYKPANVIANTLKQALKDAPKLLLTATPLQNSLLELYGLVSVVDERTFG
                     DLKSFREQFVNLNSKPAFDILQARLQPLCKRTLRRQVLPYVRFTRRLPIVQPFIPSES
                     EDRLYHLVSEYLRRDNVQALPTSQRSLMTLVLRKLLASSTFAIAGTLDALSQRLRRRL
                     QKAAEETPLEEALEEDFEALDAIAEEWGEDEAAPAPLSEADRKAIEQEIEDLEAFRTL
                     AVSITRNAKGLALLTALHIAFGKATELGAAQKAIIFTESRRTQEYLLRVLADSPWKDG
                     IVLFNGSNNDPKSKQIYQDWLQRHQGTDQVTGSRSADMRSALVDYFREQGQIMIATEA
                     GAEGINLQFCSLVVNYDLPWNPQRIEQRIGRCHRYGQQHDVVVVNFLNQNNEADQRVF
                     QLLSEKFKLFEGLFGASDEVLGAIESGVDFEKRIAGIYQRCRTSEEINASFDQLQQEL
                     STQIDEAMTRVRQLLLEHFDDEVREKLRVSDESSRQYLNRYERMLMQLTRRELGDHAE
                     FVTDSSFRLKSCPLQVDIPLGLYELPRRSGEAHLYRLRHPLAQRILEQAKSRLLAPAE
                     IAFDLSQYDGKVSTLEPFRGESGALTLTLLTIESLDQAEDYLLLAAATEAGKPLEEDA
                     ARRLFSLPARVVAELPDRTDNEGLESVLRRRQETITRAISQRNAAFFEAEAEKLDAWA
                     DDLKVGLEREIKELDRQIKEARRAATAALTLEEKLDGQKQVKALESQRNTKRRALFDA
                     QDEIDHRREQLIAEIEGKLQQKVSSQQLFAIHWNLA"
BASE COUNT         1240 a         1806 c         1775 g         1485 t
ORIGIN      
        1 aaacctcatc gcgccggaat gtcctatgcg ttctttgcgg ttaattttct cgccgttcta
       61 cttgaagacc cttgttccct catcttctgc tccagccaaa gattgacgag ggtgccggaa
      121 gaaagcccct gcttgcgagc cagcaactgg atcttctcgg acaagttctg ttctatgggg
      181 tagtaggtca cctctgattt gatgtccaca tcgaacccca ctcctctcgt cttcccccaa
      241 acatcgctga ggtcatgctt gtcccagaac tcgccaatgt ccgtgtacga gcgagcttga
      301 gagatcgagc ttttactttt tttcatatta gaacttcccg cacctcgtcc tactcgacac
      361 ggtgttcacg accgatctta tcgatgatct ctgacagcca gataatccct gaaatcttca
      421 agcatggact cctgtgtctt ctttatggtg aaatcaactc catctgcccc ggtgggggcg
      481 gagccttggg cagattctcc acacgaaggc tgtagtcatc cttgccccat tcgcaacgga
      541 cgatgacggc cttgggaatc ttcttgatcg tcaggtttgg aaagcgatcc ggcttaccgc
      601 ggaaggcaga gcacaggacc agcagtgagc ggtgctcgcc cacctcatcg ctcaaagcct
      661 gcaactggtc gtggctgagg ttctgcgtgg tgacgtagat gaagtcctgt tcagttgagt
      721 ggccatgttg ccaataaacc gtttcgctcg gcgcgtaggt gaagccttcg agcttgcaga
      781 cggcctcggc gagcatcgca gagttgtagg ccttgttgat gacccagttg tcccacttgt
      841 ctttctctaa cagcgaggga gccaggcggt agtaacgaaa cccaccgccg cccttccaac
      901 cgaccgcttc cgtgatccca cccttgtctt caccatcgat gactttcttg agcctcggga
      961 tgatgtgcgt gtggcagtgc tcgcctaact ccaccatgat ccaccgccgc cccatcttgt
     1021 gggcgacggc gccagttgta ccggagccgg caaaggagtc gagaacccag tcgccgggat
     1081 cagtagccaa ttcgagaaca cgttgcagga gcttttctgg cttgggggtc gcgaagatag
     1141 cctcggcatt gaatgccgcg acctcgcgct tggcgtcttg attatccccc acctcgttac
     1201 gcagccagat cgtcatgcag acgattcctt cctgcacctc ggaaaggaac ctcttaatgc
     1261 gcggcacgtt gtttccactt gcaccgaacc agattcgctt atccgctacc agttccttga
     1321 agcgatctgg tgatgtcccc cagcaacgcc ctctcggagg catcagtttg cgcccggaag
     1381 gagtcgttat ttcatagatg tttaagctaa ttccggttcg agcgaactga gccccacgtt
     1441 gtccaccagt aagactgata gtgaagtcgc ctgaagtcca agatcctctc aaatcattat
     1501 cggggttgga gtaacgggcg tccgactctg cggttctcct taataggttg gggcgccacg
     1561 tgttcttatc cttggcgtat acaaggatat ggtcgtggct gtccgaaagc cagattgcat
     1621 ttgcctgagg ggaaaacttt ttctgccaaa cgacattggc aataaaattc tgtcgcccaa
     1681 agacttcgtc gcaaagaact ttcatgtaat gcacttcgtt gtcatccaag gtgatccaga
     1741 ttgagccatc ctgtgcaagt agatgccgaa gaagttcgac acggtcacgg acaagagaca
     1801 accacaggga atgctcaagc ccatcgtcgt aatgactaaa ggccgaaccc gtgttgtatg
     1861 gtgggtcgat gtaaatacac ttaatcttgc ccgcgaactc ctgttcgagc gccttgaggg
     1921 cgagtaggtt gtcgccaaag atgagctggt tatcgaaaag gtcgtggtcc gtgacccggt
     1981 gtggggcgtg gtaggacctc tccgggtcct ccagcaggat gcgcggctcc agcttcggcc
     2041 ggttctcctt cccgatccac gtcagttcga gtttgggctt cgcggccatg ttgattcgtt
     2101 tatgctgctg cgtattggcg aacgtccact tgcacgccag tctgcacgac ttcttcagcg
     2161 gtcttgagca ggagtgaggt cgtggcctca tccacgtact cttcgccgca attcgcgcag
     2221 acttgtgccg gaacaccctt gatcacaagt atcgtgcctt cacgttcaag agtgatcgtg
     2281 gctttgccta attgggttgt cccgtttttg caaatcacgc acatcatcgg cgcttcctcc
     2341 tcttaaaacc ccgttcccat tcttcgggat caggctcgta ggccgtaatc acgatcgttt
     2401 ccctggtctc cgcgttctcg gctgccacca catggagtgg acgggcattc caccatccca
     2461 atatcagtcg gcttgggtac ggtgtgtcat ccggatacgt ttcgatcgtc tcgccggttt
     2521 ccagcacatg ccgcacatca ttcttgctaa tccggcgctg gaacattcgc ttgatggcgt
     2581 ggacccgaaa gaccagaggt cccacgttca tagtctggct ccgagagtat tcgttgccca
     2641 gagctccccc agggaaaagt tctccaacca ggcggtaagt gtgatctcag ccttacagtg
     2701 tggatacgta atcgttggtt ctgtcatacg cctcttcttg aggtgacccc aagcctcgac
     2761 atcggagggg aaatctccgg actcattgca tgcagtccac gcagtaccca acgcccgagg
     2821 gacggcctgt attctcacgc gtgctgaacc tgctcgcggg cctgcacgaa gcggatttct
     2881 acccgctgat ttagcgcggc ggcaattcta ttcaacatcg ccagcgagtg accttcatag
     2941 tctgcattct cgagccggca aatgacggag gccgtggtac cgaccagctt ggccagttgg
     3001 cgctgtgtca atccggcctt gacgcgcagg acgacaattt tctgggcgac gctgtcgttg
     3061 gcgcgcgcct cctcaagcgc tacgagtcgt tcaggtcggc cttcgtaata gcggcggtgg
     3121 agaatttcca cggcatcggt ggtggccgat ttcctacttt tagacatcgt caagcctcct
     3181 gataggtatg ctgctccggc gcccgctcaa acttgtgctt tcgccggagc gcttcattga
     3241 ggtcgcgtgc cggcgtcata tgttcgcatt attgcgaatg gcggtcaagt ggacacttca
     3301 aatcatgcca agttccaatg gatagcaaag agctgctggg agcttacctt ttgctgcagc
     3361 ttgccttcga tctcggcgat gagctgctcc cggcgatgat ctatttcgtc ctgtgcatcg
     3421 aagagcgctc ggcgtttggt gttgcgttga gactccagag ccttcacctg cttctggcca
     3481 tccaactttt cctccagggt cagggcggcc gtagctgcac gccgggcctc cttgatctgg
     3541 cgatccagct ccttgatctc tcgctccaag cccaccttca aatcgtccgc ccaggcgtcc
     3601 agcttttcag cctcggcctc gaaaaacgcg gcattgcgct gcgaaatcgc gcgtgtgatt
     3661 gtctcttgcc gccggcgaag gacgctctcc aagccttcgt tgtcagttct atctggaagc
     3721 tcggcaacta cccgtgccgg taacgagaag aggcgacgtg cggcgtcttc ttccagcggc
     3781 ttgcccgcct cagtcgccgc cgcaaggagc aggtaatcct ctgcctggtc tagcgattct
     3841 attgtgagga gggtgagtgt cagtgcaccg gactcccctc ggaacggttc gagtgtactg
     3901 actttcccat catattggct cagatcgaag gcgatctcgg caggtgcaag caaccggctc
     3961 ttggcctgct cgagaatccg ctgcgcgagc ggatggcgca gacgatacag atgggcctcc
     4021 ccggaacgac ggggcagttc atagagccca agcggaatgt ccacctgcaa tgggcacgac
     4081 ttcaaccgga aggacgaatc cgtgacaaac tcggcgtggt cgcccaactc gcgacgggtg
     4141 agctgcatga gcatacgctc gtaccgattc aagtactgcc gtgaactttc gtcactcact
     4201 ctgagctttt cgcgtacctc atcgtcgaag tgttccagga ggagctgcct gacgcgagtc
     4261 atcgcttcat caatttgtgt gctgagttct tgctggagct gatcgaagga ggcattgatt
     4321 tcctccgacg tgcggcagcg ctgatagatg ccggcaatgc gcttctcgaa gtccacgccg
     4381 gattcaatgg cgcccaatac ctcatcgctt gcgccaaaga ggccttcgaa cagcttgaac
     4441 ttttccgaca aaagctgaaa gacgcgctga tcggcttcgt tgttctgatt gagaaaattc
     4501 acgacgacca cgtcgtgctg ctggccgtag cggtggcagc gaccgatgcg ttgctcgatg
     4561 cgctggggat tccacggtag gtcgtaattc acgacgagcg agcagaactg gaggttgata
     4621 ccttcggcgc ctgcctcggt ggcgatcatg atctgcccct gttctctgaa atagtccacc
     4681 agggctgagc gcatatcggc ggaccgagac cccgtaacct gatcggtgcc ttggtgacgc
     4741 tggagccagt cttggtaaat ctgtttcgat ttaggatcat tgttcgagcc gttgaagagc
     4801 acaatgccgt ctttccacgg actgtcggcc aggacgcgca gcaggtactc ctgcgtgcgg
     4861 cgggattctg tgaaaatgat cgctttctgc gcggcgccga gctccgtcgc tttaccaaag
     4921 gcgatgtgca gggccgtcag cagggccaat cccttagcat tgcgggtgat cgagacggcg
     4981 agcgtgcgaa aagcttcgag gtcctcgatc tcctgctcaa tcgccttgcg gtcggcttca
     5041 gacagcggcg ccggagcagc ttcgtcctca ccccattctt ccgcgattgc gtccaacgct
     5101 tcaaagtctt cctctagcgc ctcctcaagc ggtgtttcct ccgcagcctt ctggaggcgc
     5161 cggcgcaggc gctgactgag agcgtccagc gtcccggcaa tggcgaaagt ggacgacgcg
     5221 agcaacttgc ggagcacgag cgtcatcagt gaccgttggc ttgtcgggag tgcctgcaca
     5281 ttgtcgcggc ggaggtattc ggacaccaga tgatagagcc gatcttcact ctctgacggg
     5341 atgaagggct gcacgatcgg gagtcgtctg gtgaaccgga cgtagggtag cacctgtcgg
     5401 cgaagtgtgc gtttgcagag cggctgcaga cgggcttgca ggatgtcgaa cgccggcttg
     5461 ctgttcagat taacaaactg ctcgcggaag ctcttcagat ccccgaacgt gcgttcgtcc
     5521 acgacgctga ccaacccata cagttcaagc agcgaattct gtaagggcgt agcggtgagg
     5581 agcagctttg gggcatcctt taaggcctgt ttcagggtat tggcgatcac attggcaggc
     5641 ttgtacacgt tgcgcagccg gtgggcctcg tcgatcacca cgaggtccca gtgcatgaga
     5701 ctgatctccg ctgctttgct ccgagcgaac tggtatgagc agatgatggt ctcgctgctt
     5761 tcgaacgggc ggacgtggcc ttggcggacg gcagagttgt aggactttgt ttccaggatt
     5821 cggcagggca ggaagaattt ctcctgtaac tcctggtgcc actgcttgcg gagattagaa
     5881 ggagtgatca ccaggactcg gcgttttcgt tccgcccatt tctgggcaag caccaggcct
     5941 gcctcgatgg tctttccgag ccccacttcg tcggcaagga tggcgccttt cgagaagggc
     6001 gaccgaaacg cgaacagggc ggcctcgatc tggtgaggat tcagatcaac ctgggcatcc
     6061 accaaggccc ctgccagttt ctccaggctg tcagatgggc agcgtttggt cagctcatac
     6121 gcgaaatact ttgcgtggta atccgtgagg ttcatctcgg atccgccaac gtgcataccc
     6181 gtcagagggt cttcagtcgg caaagcatac caccaatggc ggcttaggga aagggccggc
     6241 tcaccaaggg ggcacgcgag agacgaagtg gcttaaagca ccagacctga ggcagtcggg
     6301 gtgggt
//
LOCUS       DATMXI010000039         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_59849, whole genome shotgun sequence.
ACCESSION   DATMXI010000039 DATMXI010000000
VERSION     DATMXI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59849"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VN444_00755"
     CDS             complement(<1..280)
                     /locus_tag="VN444_00755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWR20373.1"
                     /translation="MIILASASPRREALLQALWVEHRIIPSLVVEAGPLPGTTTAFHA
                     ETLALRKAAEVAAQVGEGLVLGADTIVECDGRPLGKPKDLDEARWFLCL"
     gene            complement(277..546)
                     /locus_tag="VN444_00760"
     CDS             complement(277..546)
                     /locus_tag="VN444_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HWR20374.1"
                     /translation="MAKRSDSVIMLLVILILGALIGTVIGEVIAVMAPGGIVEKIFSK
                     GIHPGLSPPATLDLKVLSISFGFTVKINLSSLLGIGLALLLYRKL"
     gene            complement(685..2217)
                     /gene="cobA"
                     /locus_tag="VN444_00765"
     CDS             complement(685..2217)
                     /gene="cobA"
                     /locus_tag="VN444_00765"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWR20375.1"
                     /translation="MKGKVYLIGAGPGDPGLFTLRGVACLREADVIVYDYLANPRLLS
                     YAKPDAELIYVGKKGGNADAATQEEIGRLLIDKALAGKVVARLKGGDPFIFGRGGEEG
                     EELFQAGIPFEVVPGITSAIAVPAYAGIPLTHRDYASTVAILTGHEDPSKQTSVIAWD
                     KVATGIGTLVFLMGAGNLPTIVDKLIGHGRSKDTPVAVMHWGTKPEQETVTGTLENIV
                     ALAQMRGLGPPAVLVVGDVVRLRERLNWFERRPLFGKRILVTRTREQAGRFAELLEGQ
                     GAEVVEIPLIEIVPPESFKPLDQAIERLDIYQWVIFTSANGVDAFFHRLRELRQDARR
                     LGAARICAIGPATAEALERYAVIPDLVPAEFRAEGIIESLKPYDLQGAKILLPRAEVA
                     RDLLPKDLERQGATVEVVPVYRTVRSSTAGKVLKPLLQDQKIDLVTFTSSSTVTNFVE
                     ALAQEDLKALCEGVRVACIGPITQETAERFGLPIDIVPEHYTIPSLVDAIVQYYRPPG
                     AE"
     gene            complement(2214..3149)
                     /gene="hemC"
                     /locus_tag="VN444_00770"
     CDS             complement(2214..3149)
                     /gene="hemC"
                     /locus_tag="VN444_00770"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWR20376.1"
                     /translation="MLKLGTRGSPLALCQARLVSEDLSRQWPGLTVTIIPIKTSGDKF
                     LDVALSQIGGKGLFVKEIEEALLDNRIDLAVHSMKDLPAELAPGLRVGAVTHREDPLD
                     ALVARNGLQFTELPREARIGTSSLRRQVQLLHRRPDLQIVPLRGNVQTRLNKLETLDL
                     EAVVLAAAGLIRLGLQDRITECLPSDLCLPAIGQGALAIEIRQDDQRTAELVETLNHR
                     ETRQTTMAERAFLRHLGGSCVTPIAAFGEIEGEVLLLTGMVAGLDGKRMLRQAIRGEA
                     SAPEQVGQALAEALLAAGAGEILREIETQLSEGRT"
     gene            complement(3192..4460)
                     /gene="hemA"
                     /locus_tag="VN444_00775"
     CDS             complement(3192..4460)
                     /gene="hemA"
                     /locus_tag="VN444_00775"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWR20377.1"
                     /translation="MEIIVLGLSYKTAPIELREKFHVPESELPEVLEQLGGCGQIEER
                     MILSTCNRVETYAVVDDVDGARQFLVEFLAERHKLPSQAFESSLYLLTADQAIRHIFR
                     VSSSLDAMVVGESQILGQVKAAYAVALERDATGMILNALMDRALRVAKRVRTETGIAT
                     LAVSVSTAAIELAKKIFGDLTGRTVMLIGAGKMSELSAKHLLADGVSTVIVTNRHFDR
                     AVELAERWGGRAVPFDHAKSEMVHADIVISSTGAPHQILSKADLQEIVLQRRNRPIFV
                     IDIAVPRDVDPEANEIDNVYLYDLDDLQGVVQANLRERQREADLAEALIDREVRQFAE
                     WLASLHVVPTIVAMRKKVESIREEELQKIFAKLQDLTLEERHAISLMTGSIVNKILHE
                     PTTELKRQSAQKEGHLYVSVLRRLFGIREE"
     gene            complement(4462..5271)
                     /gene="ccsA"
                     /locus_tag="VN444_00780"
     CDS             complement(4462..5271)
                     /gene="ccsA"
                     /locus_tag="VN444_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWR20378.1"
                     /translation="MELTFTYLALLFYLSASLAYMGSLAARPGKLLQVAGILTRAGFV
                     LHTGALAVHLYQQGHPPANLADWLAFYAWATVAVYIVTEARYESKVIGSFAIPLVLLL
                     LGASAALPKTIETLSPPLKNFGVWMHVILTLLGNAAFALACCVAVMFLVQEWLLKSKH
                     PGVLFERLPSLKLLDDLCYFSLVLGFPLLTLGLLSGVLVSYSVWGSFLSWDGRQIWSS
                     ITWVFYAVLLHGRLAGGWRGRKAAILSIIGFCGVAFASLVANLIIRGTHAF"
     gene            complement(5341..5814)
                     /gene="nrdR"
                     /locus_tag="VN444_00785"
     CDS             complement(5341..5814)
                     /gene="nrdR"
                     /locus_tag="VN444_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWR20379.1"
                     /translation="MKCPYCGSLEEKVVDSREGKDGAVVRRRRQCQQCLKRFTTYERI
                     GEIHFMVIKTDGRREPFDRHKVLGGLLKATQKRPVSVVQLERIVDEIEGRLAEKAERE
                     MPSTEIGELIMERLYEIDEVAYVRFASVYRQFKDVRQFVEEVKGLRGGGRRRRAP"
     gene            complement(5836..>6220)
                     /gene="glyA"
                     /locus_tag="VN444_00790"
     CDS             complement(5836..>6220)
                     /gene="glyA"
                     /locus_tag="VN444_00790"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743642.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWR20380.1"
                     /translation="LQGHGFRLVSGGTDTHLLLIDLRGKGLTGKAAEIALDQAGITAN
                     KNAIPFDEEKPTVTSGIRIGTPAVTTRGMREGEMREIANLIADVLKDASNTAALSGVA
                     ARVKELCDSFPLYREQLALSSPHLA"
BASE COUNT         1275 a         1982 c         1771 g         1192 t
ORIGIN      
        1 acaggcatag aaaccagcgg gcctcatcca gatctttcgg cttacccaga gggcgaccgt
       61 cacattcgac gatggtgtcg gcgccaagga ccaacccctc ccctacctga gctgccacct
      121 cggccgcctt tcgaagagcc agggtctcgg cgtggaatgc cgtcgtcgtc ccgggcagcg
      181 gacctgcttc gacaaccaag ctggggatga tccggtgctc gacccacagc gcctgtagca
      241 gcgcctctcg ccggggagaa gcagaggcca ggataatcac aacttccgat agagtaagag
      301 ggccaggccg atgccaagca gactggagag attgatcttc acggtgaagc cgaaggagat
      361 gctcaagacc ttcaggtcga gggtcgcggg tggggagaga ccaggatgga tccccttgct
      421 gaatatcttt tccactatgc cgcccggcgc catgacagca atgacctctc ctattacggt
      481 gccgatcaat gcccccaata tgaggatcac cagcaacata atcacgctgt cacttcgttt
      541 agccaatcga tcctcccatg tggctccctt agaggctgtc agctttcagc catcagcttt
      601 cagaatattg tcgggttacg ctgcgctaac ccgacctacg gttgaatgct gtctcctaag
      661 gaatatcagg atgccttgct ggcgctactc tgcgcctggt ggtcgataat actggacgat
      721 ggcgtcaaca agggatggga tggtgtagtg ctctgggacg atgtcgatcg gcagaccgaa
      781 gcgttcggca gtctcttgtg tgattgggcc gatgcacgcg acgcgcaccc cttcgcaaag
      841 cgccttaagg tcttcctgcg ccagcgcctc cacgaagttg gtgacggtgg aggagctggt
      901 gaaggtgaca aggtcgatct tttggtcctg gagaagcggc ttcaagacct tcccggcagt
      961 gctcgatctt accgtcctgt agacaggcac cacttcaacg gttgcgccct gccgctcgag
     1021 atcctttggc agcagatccc tcgcgacttc ggcccgtggc agtaggatct tcgcaccttg
     1081 cagatcatac ggcttcagcg actcgatgat cccttcggcg cggaattcgg ccgggaccaa
     1141 atctgggatc acggcatatc tttccagtgc ttcggcggtt gcaggtccaa tggcgcagat
     1201 cctggcggct cccaggcgtc tggcatcctg gcgaagctcc cgtagccggt ggaaaaaggc
     1261 atcgacgcca ttcgcactgg taaagatcac ccattggtag atgtcgagcc gctcgatcgc
     1321 ttggtcgaga ggcttgaagc tttcgggcgg cacgatctcg atgaggggaa tctccaccac
     1381 ctctgcccct tgtccctcca gcaactcggc aaagcgaccc gcctgctcac gggtcctggt
     1441 cacaaggatc cgcttcccga agagtggccg ccgctcgaac cagttcagcc gctcccgaag
     1501 ccgaacgaca tcgccgacca ccaggactgc cggcggcccc agtccgcgca tctgggcgag
     1561 cgccacgata ttctcgagcg taccggtgac cgtctcctgc tcgggcttgg tgccccagtg
     1621 catgacggcg acaggcgtat ctttggaccg accatgcccg atgagcttgt cgacgatagt
     1681 tggaagattg ccggcaccca tcagaaacac caatgttccg atgccggtcg ctaccttgtc
     1741 ccaggcgatc accgacgtct gtttcgaggg atcctcatgt ccggtcaaaa tggcaacggt
     1801 cgaggcataa tcgcggtgag tgaggggaat ccctgcatac gcgggcacag caatagcaga
     1861 ggtgatccct ggaacgacct cgaaggggat gcctgcctgg aatagctcct ccccttcttc
     1921 gccgccccgc ccaaaaatga acgggtcgcc ccctttcagc cgtgccacca cctttccggc
     1981 cagagcctta tcgatcagca gacggccgat ctcctcctgt gtggccgcat cggcgttgcc
     2041 gcccttcttg ccgacgtaga tcagctcggc gtcaggcttc gcataggaca ggagacgcgg
     2101 gttggcgagg tagtcgtaca cgatcacatc ggcctcgcgg agacatgcca caccgcgcag
     2161 ggtaaaaagg ccgggatcgc caggcccggc gccgatgaga tagacctttc ccttcatgtg
     2221 cgcccctctg agagctgagt ctcaatctcc cgaaggatct ccccagcacc cgcggcaagc
     2281 agcgcctccg ccaacgcctg tcccacctgc tcgggcgcac tggcctcccc acgaatagct
     2341 tgcctgagca tccgcttgcc gtccagaccg gcgaccatcc cagtgagcag gagcacctct
     2401 ccttcaatct cgccgaaggc ggcgatcggg gtcacacagc tcccgcccag gtgccgcagg
     2461 aaagcccgtt ccgccatcgt ggtctgtcga gtctcacgat gattaagcgt ctcgaccagc
     2521 tcggccgttc gctgatcgtc ctgccggatt tcgatcgcca gcgccccctg gccgatcgcc
     2581 ggcaggcaca gatccgacgg cagacactca gtgatccgat cctgcagacc gaggcggatg
     2641 aggccagcgg ccgccagaac cacggcctca aggtcgagcg tctccaactt attcagccgc
     2701 gtctgtacat tgccgcggag gggaacgatc tggaggtccg ggcgacgatg gaggagttgg
     2761 acctgtcggc gaagactgct ggtgccgatc ctggcctcgc gaggtagttc cgtgaactgt
     2821 aggccgttcc tggccacaag cgcatcgagc gggtcttcgc ggtgcgtgac ggcccccacc
     2881 cgaaggcctg gcgccaactc cgcaggcaga tccttcatgc tgtggaccgc gaggtcgatc
     2941 cggttatcga gcagggcctc ctcgatctcc ttcacaaaga ggcccttccc gccgatctgc
     3001 gaaagggcca catcgaggaa cttatcgccg gaggtcttga tcggaatgat agtcactgtc
     3061 agaccaggcc attgtcggct caggtcttca gaaacgagtc tcgcctgaca cagcgccaga
     3121 ggactccccc gtgtacccag cttcagcata ttatacctcg cacccgtaca tgggcgcagc
     3181 aagcagcgcc cctactcctc cctgatgccg aagagtctgc gcagcacact tacgtagaga
     3241 tgaccctctt tctgggccga ctggcgtttc agctccgtcg tcggctcgtg caggatcttg
     3301 ttcacgatcg agccggtcat gagggagata gcatgacgct cctctagcgt caagtcttgc
     3361 agtttcgcaa aaatcttttg aagttcctcc tcgcggatcg attccacctt cttgcgcatg
     3421 gcaacgatcg tcgggacgac atggaggctt gcaagccact cggcaaactg cctgacctcc
     3481 cggtcgatca atgcctccgc cagatccgcc tcgcgctgcc gctcgcgaag attggcctgt
     3541 accaccccct gaaggtcatc gagatcatag aggtacacat tatcgatctc gttggcctcg
     3601 ggatcgacgt ccctgggaac ggcgatgtcg atcacgaaga tcggacggtt acggcgctgc
     3661 aggacgatct cctggaggtc agccttggac aggatctggt gcggggcgcc ggtcgaactg
     3721 atcacgatat cggcatggac catctctgac ttggcgtgat cgaacggaac ggccctgccg
     3781 ccccatcgct cggccaattc caccgcgcga tcgaaatgcc gattggtcac gatcaccgtg
     3841 ctcacaccat cagccagtaa gtgtttggcc gacaactccg acatcttccc ggccccgatc
     3901 agcatgaccg tgcggcccgt caggtcgccg aagatcttct tggccagctc gatggcggcg
     3961 gtggagaccg agacggctaa ggttgcaatc ccggtctcgg tccgcacccg cttcgcgacg
     4021 cgcaacgccc tgtccataag ggcattgaga atcatccccg tggcatcccg ttcgagggca
     4081 accgcgtacg ccgccttcac ctggcccagg atctgagatt caccaaccac cattgcgtca
     4141 aggctcgacg aaacacggaa gatatgccga atggcctgat cagccgtcaa cagatacaga
     4201 gaggactcga aggcctgcga cggaagcttg tgccgctcgg ccaggaactc cacaaggaac
     4261 tgacgcgcac cgtccacgtc gtccacgaca gcgtacgttt ccacccggtt gcaggtggag
     4321 aggatcatcc gctcttcgat ctgcccgcat ccgccgagct gctccaggac ttccggcagc
     4381 tctgactccg ggacgtgaaa cttctcccga agttcaatcg gcgccgtctt gtagctcaga
     4441 ccgaggacaa tgatttccat attagaacgc gtgtgttcct ctaatgatga gattcgccac
     4501 gagggacgca aaggcaaccc cgcagaatcc gatgatcgac agaatggcgg ccttccggcc
     4561 acgccagccc cccgccagcc gtccatgcaa caggaccgca tagaagaccc atgtgatgga
     4621 cgaccatatc tgtctgccgt cccaggacag gaaggaaccc cacacggaat agctcaccag
     4681 cacaccgctc aacagaccga gggtcagcag cgggaagccc agcacgagac tgaagtagca
     4741 cagatcgtcg aggagcttca atgagggaag gcgctcgaag agcactcctg gatgcttgga
     4801 tttgagcagc cattcctgaa ccaggaacat cactgccacg cagcaggcga gggcaaaggc
     4861 ggcgttcccg aggagggtca ggatcacgtg catccagacc ccgaagtttt tcagcggcgg
     4921 ggagagggtt tctatcgtct taggcagggc ggccgatgcc ccgagcagga ggagcaccag
     4981 cgggatcgca aacgagccga tcaccttact ttcgtagcgc gcctcggtca cgatatacac
     5041 ggcaactgtg gcccacgcat agaaggcgag ccaatcggca agattggctg gtggatggcc
     5101 ctgttggtag agatgaaccg ccagtgcccc cgtatgcaga acaaacccag cccgggttag
     5161 gatacccgcg acctggagga gcttgccggg ccgcgctgca agggagccca tatacgccag
     5221 actcgcgctc aagtagaaca gcaatgcaag gtacgtgaag gtgagttcca tcgtcgcctc
     5281 gagagccgat cttttgcgtc tattgcgtgt agcgtctatt gcgtctacgc gatgacactg
     5341 ttacggggct ctccgacgcc tcccccctcc ccggaggccc ttgacctcct caacgaactg
     5401 acggacgtct ttgaactggc gatagaccga tgcaaagcgg acgtacgcta cctcatcgat
     5461 ctcgtaaagc cgctccataa tcagctcacc gatctcagta gaaggcattt cgcgctccgc
     5521 cttctccgcg aggcggccct caatctcatc gacaatcctc tcgagctgga caacgctgac
     5581 aggccgcttt tgggttgcct tgagcaatcc ccccagaacc ttatgtcggt cgaatggttc
     5641 ccggcgtcca tccgtcttaa tgaccatgaa gtggatctca ccgatccgct cataggtagt
     5701 gaaacgcttg aggcactgct ggcattggcg tcggcggcga accaccgcgc catccttgcc
     5761 ctcacgagaa tcgacgacct tttcttcgag actgccacag tagggacact tcacaggcga
     5821 gccccttgga ggatcctacg ccaggtgggg agaggagagg gcaagctgct ctcggtataa
     5881 cgggaaggag tcacacagct ctttcacacg ggccgcgacc ccggacagag ccgcagtgtt
     5941 cgaggcatct ttcaggacgt cggcaatgag attcgcgatc tctctcatct cgccttctcg
     6001 catcccccga gtcgtcacgg ccggcgtccc gatccgaatg ccggaagtca cggtcggctt
     6061 ttcctcatcg aaggggatag cgttcttatt ggccgtaatt ccagcctgat ccagcgcgat
     6121 ctcagccgcc ttccctgtca gccctttccc tcgcagatcg atcaggagga gatgagtatc
     6181 ggttccgccg gagaccagac ggaagccatg accttgcaac
//
LOCUS       DATMXI010000040         4572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_102970, whole genome shotgun sequence.
ACCESSION   DATMXI010000040 DATMXI010000000
VERSION     DATMXI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102970"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VN444_00795"
     CDS             complement(<1..324)
                     /locus_tag="VN444_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWR20381.1"
                     /translation="MTGASRLESPGAAASSSSPLVQRLAIIGLGLIGASVGLACRARG
                     LAVQIRGADEEPDHCAQAIALGVVDQAFADAAAAVEGADLILLAVPVGAMVPTIERIA
                     PHLAPE"
     gene            complement(321..1334)
                     /gene="aroF"
                     /locus_tag="VN444_00800"
     CDS             complement(321..1334)
                     /gene="aroF"
                     /locus_tag="VN444_00800"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWR20382.1"
                     /translation="MIIILKPTATEAEIGLVVKKIESFGLAAHISKGTERTIIGAIGD
                     ERVLQEGPLEAFPFVEQVLPVLKPYKLASREFKPEGTIVNIDGVLVGGKRLAVMAGPC
                     AVESRDNLLQVARCVKAAGGHILRGGAYKPRTSPYSFQGLGEEGLAYLSEAKAETGLP
                     IATELMDPRDAPQVYQCADLVQIGARNMQNFRLLKEVGCRRKPVLLKRGLSCTVKDLL
                     LAAEYILSEGNYDVILCERGIRTFEDATRNTLDLSAIPLIKRLSHLPVVVDPSHATGK
                     WHLVSPMALAAVAAGADGIMVEVHPHPEEALSDGPQALLPSTFEKLMDDLSRVAQAVG
                     RSL"
     gene            complement(1375..1689)
                     /locus_tag="VN444_00805"
     CDS             complement(1375..1689)
                     /locus_tag="VN444_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWR20383.1"
                     /translation="MSIPNTRERKVRPTHPGEMLREDFLPDYGLTVTSFAKALGVSRQ
                     TVNELLRERRGVSPPMALCLSRLFGTSPEFWLNAQRAVDLWDATKSIKKKIERITPLH
                     AA"
     gene            complement(1703..1984)
                     /locus_tag="VN444_00810"
     CDS             complement(1703..1984)
                     /locus_tag="VN444_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR20384.1"
                     /translation="MIRTFADRHTQELYATGKSKRFPADMWKSAKRRLEYLDISTGVD
                     DLKALHSNRFHILGRDRKGQFAIAINLQWRICFRFVDCDVYDAEITDYH"
     gene            complement(2099..3220)
                     /gene="hisC"
                     /locus_tag="VN444_00815"
     CDS             complement(2099..3220)
                     /gene="hisC"
                     /locus_tag="VN444_00815"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWR20385.1"
                     /translation="MAKLLEDLASPYLHGLVPYTPGKPIGEVERELGITGAVKLASNE
                     NPLGPSPLALHALRDALLESHRYPDGGGYYLKQELARRLDLTPGHLVLGNGCNELLEL
                     MARCFLLPGDEVVIADPAFVIYGMLAHLQGCTTVRVPLKAWTHDLEAMAKAVTPRTKM
                     VFVGNPNNPTGTAVGPTELTAFMDALPADVIVVIDEAYIEYVPQEMVPDSLGYVRQGR
                     WAIVLRTFSKIYGLAGLRIGYGMAPPAMVELLERVRAPFNVNALAQRAALAALDDQAH
                     LSSSRKVNELGNAYLSGEFHRLGLEFPPSVANFLLIDLKQDGQAVADALLRMGVIVRP
                     LGGAHLKTHIRVTIGTPSENERFIRALKAVLQSECAFRS"
     gene            complement(3237..4313)
                     /gene="pheA"
                     /locus_tag="VN444_00820"
     CDS             complement(3237..4313)
                     /gene="pheA"
                     /locus_tag="VN444_00820"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWR20386.1"
                     /translation="MKITKLRHKIDQIDSKIVTLLGERAEVVFRVGQEKAKANMNLHV
                     PQREEEIFTRLMRQNTGRFPTHAIRPVFREIISACRSLEGPLKLAYFGPEGTFTHLAC
                     ARRFGVSAHFVPVRSIGDVFAEVEKGNVEYGVVPIENSSEGVVSHTLDMFVESDLKIC
                     GEILLEVSHSLLSKSGDLRKVKKVYSHPHAFAQCRKWLEANLPRVPLYEASSTAAAAK
                     LATKELTAAAIASELAASLYNLKVISRKIEDTSRNFTRFLIISQTPTAPSNRDKTSLM
                     FSIKDRVGALYRILEPFAKHQINLTKVESRPSKTKAWEYIFYLDIEGHLADEPVKAAL
                     ALLQEECLFLKILGSYPRGSSIEA"
     gene            complement(4371..>4572)
                     /locus_tag="VN444_00825"
     CDS             complement(4371..>4572)
                     /locus_tag="VN444_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HWR20387.1"
                     /translation="WLKLVVHQGRYHEIRRLCDAIGYPVLQLQRVRIGPLVLGKLAKG
                     CWRRLNLGELASIRRVVGLRTA"
BASE COUNT          939 a         1432 c         1276 g          925 t
ORIGIN      
        1 ttccggagcg agatgcgggg cgatccgctc gatggtcggc accatcgcgc ccaccggcac
       61 agccaggagt atcaggtcgg ccccttccac agcggcggcg gcgtccgcaa aggcctgatc
      121 caccacgccc agcgcgatgg cctgcgcgca atggtctggc tcttcgtcgg ctccacgaat
      181 ctgcacggcc agaccacggg cgcgacaggc cagccccaca cttgcaccga tcagacccag
      241 gccaatgatc gcaagtcgct gtacgagggg tgaagacgat gaggcagcag cgccaggcga
      301 ctccagtctt gacgctcccg tcacagcgac cgtcctacgg cctgggccac cctgctgaga
      361 tcgtccatca gtttctcgaa tgtgctgggt agcagcgcct gcggcccatc agacagggct
      421 tcctccgggt gggggtgaac ctctaccatg atgccgtcgg cgccggccgc caccgcggcc
      481 aaggccatcg gcgagaccag gtgccacttg cctgttgcat ggcttgggtc aacaaccacc
      541 ggcaagtgcg aaagccgttt gatcaggggg atagccgaca gatccaaggt gttgcgggtg
      601 gcgtcttcaa aggtcctgat cccgcgttca cagaggatga cgtcgtagtt accctccgac
      661 aagatgtact cggctgcgag caagaggtct ttcacggtac agcttagccc ccgcttcaag
      721 aggaccggct ttcgacggca cccaacctct ttcaggagcc tgaagttctg catgttccgc
      781 gccccgatct ggaccagatc ggcgcactga tagacctggg gggcgtctcg aggatccatg
      841 agctcggtgg cgatgggcaa tccggtctct gccttggcct cggacaaata ggccagcccc
      901 tcttcgccaa gaccttgaaa gctgtacggg gaggtccgag gcttgtaggc gccacctcgc
      961 aggatgtgcc ctccggcagc cttcacgcac ctggcgacct gaagcaggtt atcccgactc
     1021 tcaactgcgc acggcccggc catgaccgcc agccgcttgc caccaaccag caccccgtcg
     1081 atgttgacga tggtaccctc aggtttaaat tcacggctag ccagtttgta cggcttcagt
     1141 acgggcagga cctgttccac aaaggggaag gcctctaacg gtccctcctg tagaacccgc
     1201 tcgtcaccga tcgcaccaat gatagtccgc tctgtccctt tcgagatgtg ggcggccaag
     1261 ccgaagcttt cgatcttctt gacgaccaag ccaatctcgg cctcagtcgc ggtcggtttc
     1321 aagatgataa tcattgatta gccctcggta atcggcgcca gtttcacgcc gcagctatgc
     1381 tgcatgcaat ggagtaatcc gctcgatctt cttcttaatc gactttgtcg catcccaaag
     1441 gtcaacggct cgttgagcgt tgagccaaaa ctctgggctg gtgccgaaca gccgggatag
     1501 acacaaggcc ataggaggac tcacgccgcg acgctcgcgg agcagctcgt tgacggtctg
     1561 gcgcgacacc cccagggcct tcgcaaaact ggtcacagtc aacccgtaat ccggaagaaa
     1621 gtcttcgcga agcatctccc cggggtgagt tgggcgaacc ttcctttccc ttgtattcgg
     1681 gatgctcatg acgttcctct cttcagtggt aatccgttat ctccgcgtcg tatacatcgc
     1741 agtcaacaaa gcgaaaacag atccgccatt gaagattgat ggcaatcgcg aattgtccct
     1801 tgcgatcccg tcccaatata tgaaaacgat tgctatgcag cgctttcagg tcatccacac
     1861 ccgttgagat atccaggtac tccaggcgtc gcttcgcgct tttccacatg tctgccggaa
     1921 accgtttcga cttcccggta gcgtacaact cctgcgtgtg ccggtccgca aacgtcctga
     1981 tcacatctca actgtaatcc atcacgtgac acgtgtcaac acgggcatag catgtcggca
     2041 taacggagcg gcggctgatc cgcaggtaca gaaggcgacc gatagcttca tcggattgct
     2101 atgaacggaa ggcgcactcc gactggagca cggctttgag cgctcggatg aatcgttcgt
     2161 tctctgaggg ggtcccgatg gtcacacgga tgtgggtctt caaatgggct cctccaagag
     2221 ggcgaacgat gacgcccatt cggagtagcg catcggctac cgcctgtcca tcctgcttca
     2281 gatcgatcag caggaaatta gcgactgacg gcgggaactc cagccccaga cgatgaaact
     2341 caccggacag gtacgcattt cccagctcat tgacctttcg gctgctcgac aggtgagctt
     2401 ggtcatcgag ggcagcaagc gctgctctct gggccagggc gttgacgtta aaaggcgccc
     2461 gcacccgctc cagcagttca accatcgctg gcggcgccat cccatatccg atcctcaatc
     2521 ctgctaagcc atagattttg gagaaggtcc gaaggacgat cgcccagcgc ccctgccgga
     2581 cgtacccaag cgaatccgga accatctcct gcggaacgta ctcgatgtat gcctcatcga
     2641 tgaccacaat gacgtctgcc gggagagcgt ccataaacgc ggtcagctcc gttggcccta
     2701 ccgccgtgcc ggtgggattg ttgggattgc cgacaaagac catcttggtc ctgggggtca
     2761 cggccttcgc catggcctca aggtcgtggg tccacgcctt cagcggtacg cggaccgtgg
     2821 tgcacccctg cagatgggcc agcattccgt agatgacaaa tgccggatca gcgatgacca
     2881 cttcgtctcc cgggagcagg aagcatcggg ccatcagctc aaggagttcg ttacagccgt
     2941 tccccagaac cagatggccc ggtgtcagat cgaggcgcct cgccaactcc tgcttcaggt
     3001 aatagccgcc gccatcgggg taccggtggc tctccagcag cgcgtctcgt aaggcgtgca
     3061 gtgccagcgg cgaaggcccc agggggttct catttgacgc cagcttgacg gcccctgtga
     3121 tcccgagctc acgctccact tccccgatag gcttcccggg ggtgtacggc acgagtccat
     3181 gaaggtatgg agaggcgagg tcttccaata acttggccat aactcctgcg gttctcctag
     3241 gcctcgatcg agcttcctct ggggtacgag ccgaggatct tcaggaacag acactcctct
     3301 tgcaggagcg cgagcgccgc cttcacaggc tcgtcggcga ggtggccctc gatgtccaag
     3361 tagaagatat actcccaggc tttggtcttg gatggcctgg actcgacctt ggtcaagttg
     3421 atctgatgct tcgcgaacgg ttccaggata cgatacagcg ctccgacccg gtctttgatc
     3481 gagaacatca aggaggtttt atcgcggtta ctgggtgcag tgggggtctg acttattatc
     3541 aagaaccgcg tgaaattacg agaagtatcc tcaattttcc gagaaatgac tttaaggttg
     3601 tacaggctgg cagccagttc actcgcaatt gcagccgctg tcagctcctt tgtcgcgagc
     3661 ttcgccgccg cagccgtact cgaagcctca tagagcggaa ctcggggtag attcgcctcc
     3721 agccatttcc tgcactgggc gaaagcatgc gggtgcgagt agaccttttt taccttcctc
     3781 agatcgcctg atttggacag aaggctatga gatacctcta aaagaatctc cccacagatc
     3841 ttcaggtcag actccacaaa catatccagg gtatggctga ccacaccttc gctggagttc
     3901 tcgatcggca ccaccccgta ctcgacattt cccttctcca cctcggcaaa gacatcgcca
     3961 atcgagcgga ccggcacaaa gtgggccgat accccgaacc gccgcgcgca ggccaagtga
     4021 gtgaacgttc cctccgggcc aaagtaggcc agctttagcg gaccctccag ggaccgacag
     4081 gctgagatga tctcgcgaaa aacaggccga atcgcgtgag tgggaaagcg cccagtattt
     4141 tgccgcatca ggcgggtgaa gatctcctcc tcgcgttgcg gaacgtggag gttcatattc
     4201 gccttcgcct tttcctgtcc gaccctgaat acaacctcgg cacgctcgcc gagcagcgtc
     4261 acgatcttcg aatcgatctg atcgatctta tgccgtaact tcgtaatctt catcagactc
     4321 cccggcccag gcaggggcgc gcgcttaccc gcgtccgcca ccatgcccca tcaggcagtt
     4381 ctcagtccca ccacgcgacg gatgctggcc agctccccga ggttgagtcg tcgccagcac
     4441 cctttggcca gtttccccaa gacaagaggc ccgatacgaa ccctctggag ctgcagcacc
     4501 gggtagccga tggcatcgca gagccgtcgg atctcgtgat accggccctg gtgtacgaca
     4561 agtttgagcc ac
//
LOCUS       DATMXI010000041         4748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_102961, whole genome shotgun sequence.
ACCESSION   DATMXI010000041 DATMXI010000000
VERSION     DATMXI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102961"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VN444_00830"
     CDS             complement(<1..136)
                     /locus_tag="VN444_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWR20388.1"
                     /translation="MGKAKFERTKEHMNIGTIGHIDHGKTTLTAAITKVLHAANAKVA
                     F"
     gene            complement(240..315)
                     /locus_tag="VN444_00835"
     tRNA            complement(240..315)
                     /locus_tag="VN444_00835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Thr,seq:ggt)
     gene            complement(531..607)
                     /locus_tag="VN444_00840"
     tRNA            complement(531..607)
                     /locus_tag="VN444_00840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(571..573),aa:Gly,seq:tcc)
     gene            complement(701..785)
                     /locus_tag="VN444_00845"
     tRNA            complement(701..785)
                     /locus_tag="VN444_00845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(749..751),aa:Tyr,seq:gta)
     gene            complement(802..877)
                     /locus_tag="VN444_00850"
     tRNA            complement(802..877)
                     /locus_tag="VN444_00850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Thr,seq:tgt)
     gene            complement(962..1750)
                     /gene="rlmB"
                     /locus_tag="VN444_00855"
     CDS             complement(962..1750)
                     /gene="rlmB"
                     /locus_tag="VN444_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWR20389.1"
                     /translation="MLVGPHAVLEALRARRRHIDRIYLARERYDPRIVEIVKRARDLG
                     VPSTQEKRERLDELAKGVTHQGVLAVVSEVGYDDPFALVARIRAATPLPLLLLLDGVQ
                     DPQNLGAIIRTAEAAGADGLFISKHRAVGITPAVAKASAGAVEHLPVARVAGLPAFLT
                     WLKDQGVWILGADPSAVRSIHEIDLRASMGVVIGGEHRGLTVLVRQRCDLLARIPTYG
                     RVGSLNAAAAAAVFLFEIRRQHGIVNQASANGRESAIVSQKSSQ"
     gene            complement(1759..3342)
                     /gene="cysS"
                     /locus_tag="VN444_00860"
     CDS             complement(1759..3342)
                     /gene="cysS"
                     /locus_tag="VN444_00860"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWR20390.1"
                     /translation="MALAIYNTLSQKKEVFEPFVPGEVRMYACGPTVYDHAHIGHARA
                     ALTFDVIWRYLEFRGYKVLYVRNYTDVDDKIIQRAAERGLSFEVLAEEQIEAYRQDMR
                     ALGLRTPTEEPRATQHIREMIEIIQALIVRGVAYSVDGDVYFEVRRAPDYGKLSHRSL
                     DELRAGARVEVDPRKRDPLDFALWKASRPGEPAWKSPWGPGRPGWHIECSAMSMKYLG
                     ETFDIHGGGADLIFPHHENEIAQSEYATGKLFARYWVHNGFVNIRAEKMSKSLGNVLT
                     VRELLGRMSPCALKLFLIGTHHRAPLDFSEDALISAMAAEDRFRNVLDEVQRFRDISS
                     PTADLLRMDPSPLLDQISEAKKEFTDAMDDDFNTPRALAALFNLTRDMNVALKGADGP
                     PKQSLANALGLAGETLCGLGSVLGGLFEGAEGRGYSFIASRSLPLGLADGFPNVSQDE
                     YRGARTIIESTAASGRPVPEDKVRIIVEYRAQCRNRKDWEAADTIRAWLADLGVVVDD
                     TGDGVRWHVKATHVKTQRP"
     gene            complement(3361..3846)
                     /gene="ispF"
                     /locus_tag="VN444_00865"
     CDS             complement(3361..3846)
                     /gene="ispF"
                     /locus_tag="VN444_00865"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWR20391.1"
                     /translation="MTVGIGFDMHPLVPDRRLILGGVEIPFEKGLEGHSDADALAHAV
                     IDAILGAACAGDIGSCFGTDDPQYKDVSSLLLLREAFKRVRALGYTVSHIDATIIAEA
                     PRLAPYIAQMRANLAESVGTPVEGVSVKAATANRVGALGAGDGIACLAIASLQRSQMA
                     L"
     gene            complement(3851..4534)
                     /gene="ispD"
                     /locus_tag="VN444_00870"
     CDS             complement(3851..4534)
                     /gene="ispD"
                     /locus_tag="VN444_00870"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWR20392.1"
                     /translation="MIVTAIVPAAGAGIRFGGAVKKQFIALNGLPILSHTLRALAASK
                     ALSTVIVVVPPGEELRGREALELARIDLETEVIPGGQTRQDSVYIGLQRAKAETDLVL
                     IHDGVRPFVSREVVLATIEAAKEWGGAIAAVPVIDTIKRVDTDGFVVETLQREQLWSI
                     QTPQVFRYALLMQAHRAIRERGIVVTDDAALVERAGGMVKVVRGSYENLKITSEEDLP
                     LAGLILRRG"
     gene            complement(4631..>4748)
                     /locus_tag="VN444_00875"
     CDS             complement(4631..>4748)
                     /locus_tag="VN444_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HWR20393.1"
                     /translation="DSGRRYIGQTVDVRVTTVLPTTAGRMIFSRLKEEAEAA"
BASE COUNT         1026 a         1507 c         1208 g         1007 t
ORIGIN      
        1 cgaaggcgac cttcgcgttc gctgcgtgca ggaccttcgt gatcgccgcg gtcagggtgg
       61 tcttgccgtg gtcgatgtgc ccgatggtcc cgatgttcat gtgctctttc gtccgctcga
      121 acttcgcctt gcccatagtc acctccactc ctgaagggcg attccttctt cccttccggc
      181 actcatccgc caagggcgga ttcagtcgtc ctcatcgagt cgataccaag aagcctatct
      241 ggagcccacg accaggattg aactggtgac ctcatcctta ccaaggatgt gctctgccaa
      301 ctgagctacg tgggctggtt tgctgaggtc ccaatccacg acaggataac tcacgcacat
      361 ggggtagcta cgtgactctt tctctccgac tcctccgaat acacactatc gatcgagggc
      421 tcatccctac ccgatgcttc gccttctgtt ttatctttca cccgtaagtg agcactacga
      481 aataacggtc gagaagtaag ccgacacgaa tagccaattc atacgctctc tggagcggga
      541 aacgggattc gaacccgcga ccctcagctt ggaaggctga cgctctagcc aactgagcta
      601 ttcccgccca cgcttgtagc agttacccca gacgttcgag aatcttgtag agggagcaac
      661 tcgtctctgg tctggcactc cctgcacaag cctattccga tggtggggag aggagggttc
      721 gaacctccga aggcaacgcc agcagattta cagtctgccc cctttggccg cttgggtatc
      781 tccccagatg ctagctcctc gtggagctgg cgaagggatt tgaacccctg accggctgat
      841 tacaaatcag ctgctctgcc agctgagcta cgccagcaca caagctctta gactatgcct
      901 aatttgagaa acgcgcaagg caaatataaa gatactgtag gcaaaaagca agtaataatt
      961 tctattgaga ggatttttga gaaacaatcg cagattctcg tccattcgct gaagcctgat
     1021 ttacgatacc atgttgccga cggatctcaa acaggaagac agcagcagca gcagcagcat
     1081 taagagagcc aacgcgacca tatgtgggga ttcgcgcgag tagatcgcac cgctgtcgca
     1141 ctaacactgt cagtccccgg tgttctcccc cgatgaccac gcccattgaa gcgcgcaaat
     1201 cgatctcgtg aatggaccgt acagcacttg gatcggcccc aagaatccag acaccctgat
     1261 ctttcagcca cgtgagaaac gccggcaggc ccgcgactct cgccacgggt agatgctcca
     1321 cggccccggc agaggccttt gcgaccgccg gagtgatccc tacagcacga tgtttcgaga
     1381 taaagagccc gtctgctcct gcagcctccg cagtacgaat gatcgctcca aggttctgag
     1441 ggtcctgaac tccatcgagt aacagcaata agggcagcgg ggtggcagcc ctgattcgag
     1501 caaccagtgc aaacgggtcg tcgtagccca cctcactgac aacggccagg actccttgat
     1561 gtgtaacccc cttggctaat tcgtcaagcc gttcccgttt ctcctgtgta gagggtacgc
     1621 ctaagtcgcg ggcgcgcttc acaatctcga cgatcctggg atcataccgt tccctcgcca
     1681 gataaatccg atcgatgtga cgtcgcctcg cccgaagcgc ctctaacaca gcatgaggac
     1741 caacgagcat gtgctccgtc atggcctctg tgtcttcacg tgtgtggcct tcacgtgcca
     1801 gcggacccca tcgccggtat cgtccacaac tacccctagg tccgccaacc aggcccgaat
     1861 cgtgtcggcg gcctcccagt ccttcctgtt acggcactga gcgcgatact ctacgatgat
     1921 cctgaccttg tcttccggta ccggccgtcc agacgcagcg gtcgattcaa taatagtacg
     1981 agccccacga tactcgtcct gcgacacgtt cgggaagccg tccgccaatc caagaggtag
     2041 gcttctggaa gcaatgaacg agtagcccct tccctcagca ccctcaaata gaccacccaa
     2101 caccgacccc aacccacaca acgtctcccc cgctaggcct agggcgttcg ccagcgattg
     2161 cttcggtggt ccatcggcac ctttcagagc aacgttcata tctctggtca ggttaaacag
     2221 tgcggccagt gcgcgcggtg tattaaaatc atcatccatc gcatccgtaa actctttctt
     2281 cgcctcgcta atctggtcca aaagaggtga gggatccatg cgtaaaaggt cagccgttgg
     2341 actcgagata tctcggaaac gctgcacttc atccagtaca ttacgaaatc gatcctctgc
     2401 ggccatagcg ctgatcagcg catcttccga aaaatctaaa ggtgcccggt gatgggtgcc
     2461 tatcaaaaag agtttgagcg cacagggact catgcgcccg aggagctctc ggaccgtcag
     2521 gacattgccc agcgacttcg acattttttc ggcgcggatg ttaacaaatc cgttatgaac
     2581 ccagtagcgg gcaaacaact taccggtggc gtattccgac tgcgcgatct cgttctcgtg
     2641 atggggaaag atcagatcag cccctccgcc gtggatgtcg aaggtctcac cgaggtattt
     2701 catcgacatg gctgagcact cgatatgcca acctggccta cctgggcccc acgggctctt
     2761 ccacgcaggc tctcctggcc gcgatgcctt ccacaaggca aaatcgagag gatcacgctt
     2821 tcgcggatcc acctccaccc ttgcccctgc tcgcagctcg tcgagacttc gatgggagag
     2881 tttcccgtag tcaggcgctc gcctgacctc gaaatagaca tcgccatcca cggaatacgc
     2941 gacccccctc actatcaagg cctggatgat ctcgatcatc tcccggatat gctgcgtagc
     3001 ccgaggctcc tccgtcggcg ttctgagacc cagagctcgc atatcctggc gataggcctc
     3061 gatctgctcc tcggccagca cctcaaacga cagcccccgc tctgcggccc gttggataat
     3121 cttatcgtcc acatccgtat aattacgcac gtagaggacc ttgtaccctc ggaactccag
     3181 gtagcgccag atcacatcga aggtcagggc ggcgcgcgcg tgaccgatgt gggcatgatc
     3241 gtacacggtc ggtccgcagg cgtacatccg gacctcgccg ggtacgaacg gttcaaacac
     3301 ctcttttttc tggctcaggg tattgtagat cgctagcgcc atatgactac ccccgtcatg
     3361 ctacagcgcc atctgagagc gctggagaga ggcaatggcg agacaggcaa tgccgtcacc
     3421 tgccccaagc gcgcccaccc gattcgccgt cgccgccttg acgctcactc cctcgaccgg
     3481 ggttccgacg ctttctgcaa ggttggcccg catctgggca atatacggcg caagccttgg
     3541 ggcttctgcg atgatggtcg cgtcgatgtg actcaccgta tagccaaggg cccgcactcg
     3601 tttgaaagcc tccctgagga gcaggagact cgatacgtcc ttatattgag gatcgtccgt
     3661 cccaaagcag gagccgatat caccggcgca cgccgcacca agtatggcgt cgatcaccgc
     3721 atgggccagg gcgtccgcat ccgagtggcc ttcaaggcct ttctcgaatg ggatctccac
     3781 cccacccagg ataagacgtc gatcaggtac taatgggtgc atgtcgaacc cgataccaac
     3841 tgtcattggg ctatccacgc ctcaggatca ggccggccag gggtaaatcc tcttcgctcg
     3901 taattttgag gttctcgtaa cttcctctca caaccttgac cattccccct gctcgctcta
     3961 ccagtgcggc atcatcggtg acaacaatcc cacgctcccg aatcgcccgg tgagcctgca
     4021 tcaagagtgc gtaacggaag acctggggag tctggatcga ccagagctgt tctctctgca
     4081 gcgtctctac cacgaagccg tcggtgtcta ctcgcttgat ggtatcgatg acgggcacgg
     4141 ccgcgatcgc cccaccccat tctttcgcag cctctatggt agccagcaca acctcacgcg
     4201 aaacgaaagg gcgaacgccg tcgtggatca atacgagatc cgtctccgcc ttcgccctct
     4261 gcaaaccgat atagactgaa tcctgccgtg tctgtccccc tgggatcacc tccgtctcca
     4321 gatcaatcct ggccagttcc agtgcttctc gacctcttaa ctcttccccg ggaggaacta
     4381 caacgatgac ggtcgaaagc gccttggatg ccgcaagcgc tcgcaatgtg tggctgagta
     4441 tcggaagacc attcagcgcg ataaactgct tcttgaccgc tcccccgaat cggatccccg
     4501 ctcccgccgc gggaacaatt gccgtaacaa tcatagggta tagtgtctag ggcatcgggt
     4561 atcgggtctg aaatctccgg ggcgtacgtt tccgcctaaa ccctaatccc tagaccctga
     4621 accctgtcct tcaagcggcc tcggcctctt ccttcaggcg tgagaagatc atacgcccag
     4681 ccgtcgtcgg cagcacagta gtaacgcgta catcgacagt ctggccgata tatcgacggc
     4741 cgctatcg
//
LOCUS       DATMXI010000042         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_278013, whole genome shotgun sequence.
ACCESSION   DATMXI010000042 DATMXI010000000
VERSION     DATMXI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278013"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1814
                     /locus_tag="VN444_00880"
     CDS             261..1814
                     /locus_tag="VN444_00880"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWR20394.1"
                     /translation="MISTAELAKAVGQALEYLRSAEQVAEAEVFASSNGNLLARLNYT
                     SHIPCNGVEEPKSSESYGLGITVVFKTEAGITIGFGSEPSDLSLDGVKRALEKARRGA
                     VVDPEFSSLPRATGERRRLRRYHDPQLMKIRDEDLVEVGWRILQGGLKTFAASPQLAE
                     LAGGKGQINALGLIVGGDVTIQQERIAIASTTIPEVQTDESTLIMAFITAMVEAKASK
                     GSGCSAGTRLDQFTDDAGVEAAHNALAAIGGERIKGGEYTVIFGRQPVADLLNNLILP
                     SLSLSAFYSDSSPFMGRLGKRIASKHLTLYDHGNKRGLMGSKGITCEGLSTGKTRLIQ
                     RGRLVGLLTNHYEQQRIVRDPRGTEKLGVDPNQHGNAIVPRNGFRFWAGGGRQFGVQA
                     AVASTNAVVQGGDQSLDELIATVKDGLLIGRIWYTYPINGLRAGDFTCTVVGDSYIIK
                     DGRITTPLKPNTVRINDNIHTILSNIIAIGKEVRETLVWAADEVVYTPEIAVRGVKLD
                     EIASFMEQL"
     gene            1880..>2488
                     /locus_tag="VN444_00885"
     CDS             1880..>2488
                     /locus_tag="VN444_00885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWR20395.1"
                     /translation="MAAAPQTKAILFDFGGTLDADGVAWKDRFYPLYLAEHVAADRDR
                     YDQAYYVGDDALVARGFSDYSFRESLLFQITGVLEALNVADARVASRLCDRFLQDSLD
                     KIRSNLTILRELSARFRLGIISNFYGNLERVCLESGLTPLMSVMVDSARVGFTKPDPR
                     IFQVALTQLQLDPREAIFVGDSLSRDMEGAKRLGMPHIWLVPD"
BASE COUNT          549 a          692 c          760 g          487 t
ORIGIN      
        1 ctgatgaagg tggatgcggt gggaagcgac ttccgtctct atccgatccc caattgcggc
       61 aagggccagc cgatgcagac caagcggctc ggtaacgggg gccccaccat gcgcagccgc
      121 gcccgcctga caggagcgta aaagggtaga gggtataggg tgaagggtat agggtatgag
      181 gctgagaaaa cacccaacac actctttact aaaccctaac ccctaaaccc tcaaccctgg
      241 tattgctggg aggcgacccg atgatctcaa ccgctgaact tgctaaggcc gtggggcagg
      301 ccttggagta tctgaggtca gccgagcagg tggccgaggc cgaggtgttc gcatcaagca
      361 acggcaacct cttggcccgg ctgaactaca cctcgcacat cccctgcaat ggtgtcgaag
      421 agccgaaatc aagcgaatcg tacggccttg ggattacggt agttttcaag acagaggccg
      481 gtatcacgat cgggtttggc agcgaaccga gcgatctgag tctcgatgga gtgaaaagag
      541 ccctcgagaa ggccaggcgc ggcgccgtcg tcgatccaga attctcgtcg ctgccgagag
      601 ccacgggtga gcgacggagg ctccgtcgct accacgaccc tcaactgatg aagattcggg
      661 acgaggatct ggttgaggtc ggctggagga ttttgcaggg gggactgaag acctttgcag
      721 cctcccctca acttgccgaa ctggcgggtg gtaagggcca gatcaatgca ttaggcctca
      781 ttgtaggtgg cgatgtgacc atccagcagg agcggatcgc cattgcctcg accaccatac
      841 ccgaggtcca gaccgacgaa tcgactctga tcatggcctt catcactgca atggtcgagg
      901 ccaaggcctc caagggcagt ggctgctccg ccggcacccg cctggaccag ttcactgacg
      961 acgccggtgt agaagccgca cacaacgccc tcgcggcgat tggcggagag cggatcaagg
     1021 gtggggagta tacggtcatc tttgggcgtc agcccgtagc cgatctcctg aacaatctga
     1081 tcctgccttc tctcagcctc agcgccttct acagtgacag ctctccgttc atgggacggc
     1141 tcgggaagcg aatcgcgtcg aaacatctta ctctctacga ccatggcaat aagcgtgggt
     1201 tgatgggcag caagggaatc acctgcgaag ggctatcgac cgggaaaaca cgcctgatcc
     1261 agcggggcag attggtaggg ctgctcacca accattacga gcagcagcgt attgtaagag
     1321 atcccagggg cacagagaag ctgggggtgg acccgaacca gcatggcaac gcaatcgtac
     1381 cgcggaacgg tttccgattc tgggctggtg gcggtcgaca gttcggtgtc caagccgcag
     1441 tcgcctcaac caatgctgtc gtacagggtg gagaccaaag cctcgatgag ctcatcgcca
     1501 ccgtaaagga cggcctacta atcgggcgga tctggtatac atatcccatc aacgggctgc
     1561 gggccggcga cttcacctgc acagtggttg gtgactctta catcatcaag gacgggcgga
     1621 tcacgacgcc gctgaaaccc aacacggtac ggatcaacga taatatccac acgatcctct
     1681 ctaatatcat cgcgatcggt aaggaggtca gggaaacctt ggtctgggcg gcagacgagg
     1741 tcgtctatac gcccgagatc gccgttcgag gcgtcaagct cgacgagatc gcctccttca
     1801 tggagcagct ctgaggggtt tgagactgcc ttgcgatgaa gacgggagtc gttgaaacgc
     1861 ccagggaggt tactggctga tggctgcagc accacagaca aaagctattc tcttcgattt
     1921 cgggggaacc ctcgatgccg acggagtcgc ctggaaagat aggttctatc cgctctatct
     1981 cgctgagcat gtggcggcag acagggatcg atacgatcaa gcctactatg ttggcgatga
     2041 tgctctggtg gcgcggggat tctcggacta ctcgtttcga gagagcctgc tgtttcagat
     2101 tacgggcgta ctggaggcgt tgaacgtggc tgacgcgagg gtcgcgtcgc gtctatgcga
     2161 caggtttctt caggactcgc tggataagat tcgttctaac cttacgatcc tgcgggagct
     2221 atcagcacga tttcggctcg gcatcatctc gaacttctat ggcaatctcg aacgcgtctg
     2281 cctcgagagc ggactcaccc cgctcatgag tgtaatggtt gattcggcgc gcgtcgggtt
     2341 caccaagccg gaccctagga tctttcaggt cgcgctgacg cagttacaac tcgacccgcg
     2401 agaggcgata ttcgtcggcg actcattatc tcgcgacatg gagggggcga aacggctcgg
     2461 gatgccccat atctggctgg tcccagac
//
LOCUS       DATMXI010000043         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_190334, whole genome shotgun sequence.
ACCESSION   DATMXI010000043 DATMXI010000000
VERSION     DATMXI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190334"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VN444_00890"
     CDS             complement(<1..495)
                     /locus_tag="VN444_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWR20396.1"
                     /translation="MSQTSGPILSILIAIPMCGALLLACIDGAREALIKRLTLAVSGL
                     DLLLSLIVYVYFDAAQAGMQFVERASWIPSIGSSYLLGVDGISMPLLLLTTFLTPIAI
                     LASFSGVASRVKAYMICMLLLLSGMIGVFVALDLVLFYVFWEGMLIPMYFLIGVWGGP
                     RRIYA"
     gene            complement(511..2403)
                     /gene="nuoL"
                     /locus_tag="VN444_00895"
     CDS             complement(511..2403)
                     /gene="nuoL"
                     /locus_tag="VN444_00895"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWR20397.1"
                     /translation="MIWLIPVVPLTGSLVVGLMGRRMPRGLVTGIACGATSLSLLLSL
                     MAFVRLLEMPAEGRLLRSSIGSWIASGDFSVSFGFLFDPLSAIMALVVCGVGLLIHIY
                     SIGYMNEDRDYHRFFSLLNLFLAEMLVLVLADNYLLLFVGWEGVGLCSYLLIGFWFER
                     PAAASAGTKAFLVNRIGDGAVVVGMIWMILLFGSLDFQTVFAAAPTVLAYGSMTALLL
                     TLLLFIGATGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYLVARSAPLFQLA
                     PVSLEIVAWVGGLTALYAASIALVQTDIKRIIAYSTISQLGYMFLGLGVGAYAAGIFH
                     LMTHAFFKALLFLSAGSVIHALAGEQDIGKMGGLRKSLRVTTGSFLVGALANAGIAPF
                     AGFWSKDEILFAAYTSGHRLLWVIGVLTASGTALYMFRLYFLAFEGKSRLDAHTLRHV
                     HEAPWSMRLPLVLLALGSATVGFVGFPPESGPFQHFLGSVFPVPAHEASAEPSSGVVL
                     AVATLVMALLGIGVAFWYYLRDPARPEALAVRYPALHRTLLHKYWVDELYNKAVIHPT
                     ITFARAVSEWFDTRIVDGSVNGIATLATRAGSMLRRLQTGHVPTYILSILVGAVVLLG
                     CLAFYG"
     gene            complement(2431..2742)
                     /gene="nuoK"
                     /locus_tag="VN444_00900"
     CDS             complement(2431..2742)
                     /gene="nuoK"
                     /locus_tag="VN444_00900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWR20398.1"
                     /translation="MTGSVPLSAYLILSAVLFAIGVTGVLIRRNALVIFMSIELMLNA
                     VNLTFVAFSRFLHSMDGQIIVLFVMAVAAAEVAVGLAIIIALYRNKESLNVDEINLLK
                     G"
     gene            complement(2884..3390)
                     /locus_tag="VN444_00905"
     CDS             complement(2884..3390)
                     /locus_tag="VN444_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWR20399.1"
                     /translation="MEWLVFVPLAAMALITAVLVIVLRNPVYCALSLIGTFFALSGIY
                     LLLQAQFIAVVQVIVYAGAIMVLFLFVVMLLDLGHELPAWLQRDRPRILLGICVTLLL
                     LVELAIPIGFRTPHGPQGPYTSELVGAVGNTQLVGRLLFTEFLIPFEITSIILLIAII
                     GVMVLARR"
     gene            complement(3393..4379)
                     /gene="nuoH"
                     /locus_tag="VN444_00910"
     CDS             complement(3393..4379)
                     /gene="nuoH"
                     /locus_tag="VN444_00910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWR20400.1"
                     /translation="MPEGTFFVLMVAKIVVVFGLMLLSVTYLTWLERKILSDIQVRLG
                     PMRVGPHGLLQPIADGLKLLFKEEIVPQAADRTLYLLAPVLALIPAFISFAVIPFGDR
                     IRLFGQSIDLVIADVNIGLLYVFGVASLGIYGIVLGGWASNNKYALLGGLRASAQMIS
                     YELSLSLSVVGVVMLSQSLSLVQVVDAQARVWFIVLQPIGFLIFLICAVAEINRAPFD
                     LPEAETELVAGFHVEYSSMKFAMYFMAEYANMITVSAMATTLFLGGWRGPWLPPVVWF
                     LIKLYLFIFLFIWLRGTLPRFRYDQLMRFGWKVLLPIALVNIMVTAVFVALR"
     gene            complement(4447..>4758)
                     /locus_tag="VN444_00915"
     CDS             complement(4447..>4758)
                     /locus_tag="VN444_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWR20401.1"
                     /translation="GEVVSRFYHHESCGQCTQCREGTAWLHKTLRRIEEGRGRTADLD
                     LLLDICDNMKGKTICPLSDAAAMPIESYLKYFRDEFEQHIVDRACPFQSAYSVPSIIA
                     S"
BASE COUNT         1206 a         1433 c         1354 g          765 t
ORIGIN      
        1 ggcatagatt cgcctcggac cgccccagac gccgatcaga aaatacattg ggatcagcat
       61 tccctcccag aagacgtaga agagcaccag atccagggca acaaacactc caatcatgcc
      121 gcttaggagc agcagcatgc agatcatata cgcttttacc cggctcgcga ccccggagaa
      181 agaggcgagg atggcgatag gcgtgagaaa cgtcgtgagg agcaacagcg gcatgctgat
      241 cccgtcaact ccgagaagat agctgcttcc gatcgaaggg atccacgacg cccgctcaac
      301 gaactgcatg ccggcctggg ctgcatcaaa gtacacatac acgatcaggg aaagcagaag
      361 gtctagaccg gagacggcca gcgtaagcct cttgatgagt gcttctcgcg ctccatcgat
      421 acatgcgagc aaaagagcgc cacacatcgg aatggcaata agtattgata agatcggacc
      481 gctcgtctga ctcatacctt cccctgtgcg ctatccataa aacgccaggc atcccaagag
      541 gaccacagca cccaccagga tcgaaaggat ataggttgga acatggccgg tttgaagtcg
      601 cctcagcatg ctgccggccc gagttgcaag tgtcgcgatg ccgttgactg acccgtcgac
      661 tatacgcgta tcgaaccact ccgatacggc gcgagcgaag gtaatggtcg ggtggatcac
      721 ggccttattg tacagctcat caacccaata tttgtgaagg agggtgcgat gcagcgcggg
      781 ataccgcacg gcaagcgcct cgggtctggc aggatcccga agatagtacc aaaaggccac
      841 accgatcccc agcagcgcca tgacaagggt cgcaaccgca agcacgaccc cggagctcgg
      901 ctctgccgag gcctcatgcg ccgggaccgg gaagaccgaa cccaggaaat gctgaaacgg
      961 gcctgactct ggcggaaagc cgacaaaccc gacggtcgcc gacccgagtg ccaacagcac
     1021 cagcggcagg cgcatgctcc acggcgcctc gtgcacgtgt ctgagcgtat gagcgtcaag
     1081 cctcgacttc ccctcaaagg caaggaaata gaggcgaaac atgtacaatg ccgtacccga
     1141 cgcggtcagt accccaatga cccagagcag ccgatgtccc gaggtatagg cggcaaagag
     1201 gatctcgtcc ttgctccaga agccggcgaa tggggcaatg ccggcgttcg ccagtgcgcc
     1261 cacgagaaag ctgccagtcg tgacacggag ggacttccgc aggcccccca tcttcccgat
     1321 atcctgctcg ccggccagcg catgaatcac cgatccggcc gataggaaga ggagcgcctt
     1381 gaagaaggca tgggtcatca ggtgaaagat ccctgcggcg tacgccccga ctcctaagcc
     1441 taaaaacatg tagccgagct gggagatggt cgagtaggcg ataatccgct tgatgtcggt
     1501 ctgaaccagg gcgatgcttg cggcatagag agccgtgagg ccaccgaccc aggccacgat
     1561 ctccaggctc acgggcgcga gctgaaacag gggagccgaa cgggccacca gatagacgcc
     1621 tgccgtcacc atggtagcgg catggataag cgccgagacc ggcgtaggtc cctccatcgc
     1681 atcaggtaac cagacataca atgggagttg ggccgacttg ccggtcgccc cgatgaacag
     1741 gaggagggta agaagcagtg cggtcataga gccatacgca agtacggtcg gcgctgctgc
     1801 aaacacggtc tggaagtcca gtgacccgaa cagcaggatc atccagatca tccccacaac
     1861 tacggcgccg tcgccgatcc ggttcaccag gaaggccttg gtcccggcgg aggccgcagc
     1921 cggtcgttcg aaccagaaac cgatcaacag gtacgaacag agacctaccc cctcccatcc
     1981 cacaaagagc aggaggtagt tatcggccag gaccaggacc agcatctcgg ccaggaagag
     2041 attgagcagc gagaagaagc gatggtagtc gcggtcctca ttcatataac cgatcgagta
     2101 gatgtggatc agtaggccca cgccgcacac caccagcgcc atgatagccg agaggggatc
     2161 gaacaggaac ccgaacgaga ccgagaaatc gccggaggcg atccaggagc cgatggagga
     2221 gcggagcagg cgtccttccg ccggcatctc aaggagacgg acaaaagcca tcagggaaag
     2281 gagcaacgaa agcgatgtgg cgccgcaggc gatgcccgtc accagccctc tcggcattcg
     2341 gcgacccatc agaccgacga cgaggcttcc cgtcagcgga acaacgggaa tcagccaaat
     2401 catcgtacgc accccgcacc aatcatcgca ctagcccttc aggagattga tctcgtccac
     2461 atttaaggac tctttatttc tgtacagcgc aatgatgatg gccagcccta ctgctacctc
     2521 ggccgccgcc accgccatca caaacaacac aatgatctgt ccatccatcg agtggagaaa
     2581 cctcgagaac gccacaaagg tgagattcac cgcattcagc atcagctcga tcgacatgaa
     2641 gatcaccagc gcattacgcc ggatcagcac cccggtcacc ccgatggcaa agaggacagc
     2701 gctcaagatc aggtacgcgc tcagtggcac ggatccagtc atggcgcctc acaccgcgta
     2761 ccccacgccc atgaaatccc cccatccccc cttttccaaa ggggggcgag gggggatttg
     2821 ccttgaccgt tgaacgttgc acctcgggca gccacaaggg ctgcccctac attccgcact
     2881 tcactacctc ctggccaaga ccataacgcc aataatggca atgagaagga tgatagaagt
     2941 aatctcaaat gggatgagaa attcagtgaa taagagacgg ccgacgagct gagtatttcc
     3001 gacagcgcct acgagttcgg acgtgtaggg accctgggga ccgtgaggag tcctgaagcc
     3061 aatagggatc gctaactcaa caagcaggag cagagtgaca cagatgccga gtaggattct
     3121 tggccgatct cgctgcagcc aggcgggtag ttcatgaccc aggtcgagca gcatgaccac
     3181 gaacaggaac aggaccatga tcgctccggc gtagacgatc acctgcacga cggcgatgaa
     3241 ctgcgcctgc aagagcagat agatccccga tagcgcgaag aacgtcccaa taagcgagag
     3301 ggcgcagtag accggatttc gcaggacgat gaccaggacc gcagtgatga gtgccattgc
     3361 ggccagcgga acgaacacca gccattccat ccttacctca acgccacaaa cacagccgtg
     3421 accataatat tgaccagcgc aatgggcagc agaaccttcc acccaaatcg catcagttga
     3481 tcgtatctaa agcgcggcag cgtaccccta agccagatga agaggaaaat aaacagatag
     3541 agcttaatga gaaaccagac caccggtggc aaccacggcc ccctccaccc gcccaggaac
     3601 agcgttgtcg ccatggccga tacggtgatc atgttggcgt actcagccat aaagtacatg
     3661 gcgaacttca ttgagctgta ctcgacgtga aaaccggcca ccagttccgt ttcggcctcc
     3721 gggagatcga agggggcgcg attgatctcg gcgacagcac agatcaggaa gatgagaaac
     3781 ccgatcggct gaagaacgat aaaccacacc ctggcctgag cgtccacaac ctgcaccagg
     3841 ctcagcgatt gcgacagcat caccacgccc accaccgaca gactcaggga aagctcatag
     3901 ctgatcatct gggccgaggc acgcagcccc ccaagcagcg cgtacttgtt gttcgaggcc
     3961 caccctccca ggacgatccc atagatcccg agagaggcga caccgaatac atacagcagc
     4021 ccgatattga cgtcggcgat gaccaagtcg atgctctgtc cgaagagtcg gatccggtct
     4081 ccgaacggga tgacggcaaa ggagatgaag gcagggatca gcgcaagcac tggggccagg
     4141 aggtagagtg tccggtcagc ggcctgggga acgatctcct ccttgaacag gagcttgagc
     4201 ccgtcggcga tcggctgtag caggccgtgg gggccgaccc gcatcggacc gagccggacc
     4261 tggatgtcgc tgaggatctt gcgctccagc caggtcaggt aggtgacgct tagcaacatc
     4321 agcccgaaga ccacgacgat cttcgctacc ataaggacga agaaggtgcc ctccggcatc
     4381 attcctccgc gtctattgcg tcattgcgtc ggcgcgtctg ttgcgtcggt ggcatccctt
     4441 gtgttgctat gacgcgatga tgctggggac gctgtaggcg ctctggaacg ggcaggcgcg
     4501 atccacgatg tgctgctcga attcatcacg aaagtacttc aggtagcttt caatcggcat
     4561 ggcggcagca tcgcttaagg ggcagatggt tttgcctttc atgttgtcac aaatatccaa
     4621 gaggaggtcg aggtctgccg ttcggccgcg accctcttcg atccttctca gcgtcttgtg
     4681 cagccacgcc gtcccctccc ggcactgcgt gcactgcccg cacgattcgt gatgatagaa
     4741 tcgagaaacc acctctcc
//
LOCUS       DATMXI010000044         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_301448, whole genome shotgun sequence.
ACCESSION   DATMXI010000044 DATMXI010000000
VERSION     DATMXI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301448"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VN444_00920"
     CDS             complement(<1..1284)
                     /locus_tag="VN444_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR20402.1"
                     /translation="MAKTKILIAEDEPASRAGLQELLTSWGYTVTAAANGQEALEKAS
                     ELQPSLIIADLVMPKMDGLTLLRTLKNDATLPSLIILTGQGTIETAVQAMQEGAYDYL
                     TKPVDIGRLRVLVEKALERGAVLKEVKLLRHQVRHLGRFGRLVGNTRAMQEVYRLLEL
                     SAPSTAPVFIWGESGTGKELAARTIHDLSPRKQGPFVPINCAAIPETLLESEIFGHEK
                     GAFTGATERRMGCFELADGGTIFLDEVAEMKVSTQAKFLRILQEGSFRRLGGAREIHI
                     DIRVVAATNKDPSQTVQDGLLREDLYYRLNVFSIHLPPLRERREDLSLLIRSFIEEFK
                     EKYGTAVCSVDAGALALLTRHDWPGNVRELRNVLERAVLVAQGNMITSADLPPNFPSQ
                     RPGPTPEINIPVGFTIDAAEKALILTTLGRTNQNKT"
     gene            1414..1842
                     /locus_tag="VN444_00925"
     CDS             1414..1842
                     /locus_tag="VN444_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWR20403.1"
                     /translation="MRSTGRLTISKLMQRFQIWSLAASVMVLTGCAMLFPEIVPPAGA
                     PAEAKRLSFTMIARNYRCEPSVIVVDREGRSVLVKVSIRSDGATHVFSIPDLEIRRYL
                     EPGQETTIEFLAERSGVFDFGCTRFPLITPLDHKGKLAIK"
BASE COUNT          411 a          579 c          584 g          453 t
ORIGIN      
        1 ggtcttgttc tggttcgtgc gtcccagcgt tgtgagaatc aaggcttttt ctgccgcatc
       61 aatcgtgaac ccgactggaa tgttgatctc cggggtcggc ccaggacgct gactggggaa
      121 gttcggcggc agatcggctg aggtaatcat gtttccctgg gccacaagga cagcccgctc
      181 cagcacattc cgaagctccc ggacgttgcc gggccaatcg tgcctggtta agagcgcgag
      241 cgctcctgca tccacgctgc acaccgccgt gccatatttc tctttaaact cctcgataaa
      301 tgatcggatg aggagcgaga ggtcctcgcg ccgctcccgc aggggtggga ggtggatact
      361 gaacacgttt agccgatagt agaggtcctc gcggagcaag ccatcctgga cagtttgtga
      421 cggatcttta tttgttgcgg cgactacccg gatatctatg tggatttccc tggctccacc
      481 cagtcgacga aagctgccct cctgaaggat ccggaggaac ttggcctggg tggagacctt
      541 catctccgcg acctcatcaa gaaagatggt cccgccatcc gccagctcaa agcatcccat
      601 tcgccgctcg gtagcgccgg taaacgcccc tttctcgtga ccgaaaatct cactctccag
      661 cagcgtctcc ggaatggcgg cgcagttgat cgggacgaag gggccttgct tccgtgggga
      721 gaggtcgtga atcgtcctgg ccgccagttc ctttccggtt ccgctttccc cccagatgaa
      781 gaccggcgca gtgctaggcg ccgacaattc aagcagccgg tagacctcct gcatagcacg
      841 cgtattgcca accaatcggc cgaaccggcc caggtggcgg acctggtgac gcagcagctt
      901 gacctccttc aacaccgccc cacgctcgag agccttctcc acaaggacgc ggagccgccc
      961 gatatcgacc ggcttagtga ggtagtcgta ggccccttct tgcattgcct ggaccgcagt
     1021 ctcgatggtc ccctggccgg ttaaaatgat cagggagggg agggtagcgt cattcttgag
     1081 agttcgcagc agcgtgagac cgtccatctt cggcatcacg agatcagcga tgatgagaga
     1141 gggttgcaac tctgacgcct tctccagagc ctcctgaccg ttggcagcgg cagtgaccgt
     1201 gtaaccccag ctcgtgagaa gctcctggag ccccgctctg gaggcaggtt cgtcctctgc
     1261 gattaagatc tttgtttttg ccatagtgat tctgagagaa aaggttttcg acaagcctgc
     1321 tttatcatag gggctttccc agattttgtg aagtattgtt cgttgaccct gcttgaaaat
     1381 ctctacagtc ctgtggtaat gtgcggcatc aagatgcgaa gcactgggag gctcacaatc
     1441 agcaagctca tgcagcgatt ccagatctgg tcattggcgg cttccgtcat ggtcctgacg
     1501 ggctgcgcca tgctttttcc tgagatcgtc cctccagccg gggcccccgc cgaagcgaag
     1561 cggctttcct tcacgatgat cgcccgcaac taccgctgtg agccctccgt tatcgtggta
     1621 gaccgggaag ggagatcggt gctggttaag gtaagtattc ggagcgacgg cgccacacac
     1681 gtcttttcta tccctgactt agagatcaga cgctatctcg agccgggtca agaaacaacc
     1741 attgagttcc tggcagagcg ttccggagtc ttcgatttcg gctgcacacg gtttccgctg
     1801 atcaccccgc tcgaccacaa gggtaagctg gccatcaaat gaggtatggc agtgaggagg
     1861 tcacgtatac tgagctactc cattgccgaa ggatcaattc ttacgagcgg ctgaggtgct
     1921 ctccactgtc aagacgaaaa tacggattgg ttaaggtaga gatagagcgc ctgagccggg
     1981 gtggtaagcc gcgtcttccc gagatagatc tcgatggggt gcaatag
//
LOCUS       DATMXI010000045         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_81939, whole genome shotgun sequence.
ACCESSION   DATMXI010000045 DATMXI010000000
VERSION     DATMXI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81939"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VN444_00930"
     CDS             <1..1163
                     /locus_tag="VN444_00930"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWR20404.1"
                     /translation="ARNHGARVLGFYDGFKWLARGDTSHMVEIEIDEVSRIHFEGGSI
                     LRTSRENPAKSPDTLRNVAEALDKLEVSCLLTIGGDDTAFAACRLSEAVKGRVAVAHV
                     PKTIDNDLPLPQEVTTFGFTTACNLGKDIVQNLMEDAVTTNRWFFVTVMGRRAGHLAL
                     GIGGAAGATLTVIGEEFPEPRIPLQTLVDILEGAIIKRRASGKGHGVAILSEGLADKL
                     DPADFGSVERDGYGNVRLSELVLVQVLKERVTESLRARGVDVTIIAKDLGYELRCAPP
                     GALDIQYCRSLGYWATRFLLDGHTEAMVTIQGGKMVPIPFREMLDHRTGKIRVRYVDI
                     DSESYQTLRAYMIRLEVQDFETPGQIETLAHGGHLEQAEFVSRFGYLGDRRS"
     gene            1213..1821
                     /locus_tag="VN444_00935"
     CDS             1213..1821
                     /locus_tag="VN444_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20405.1"
                     /translation="MRYWRRSIQRMIFGVVVVLVPCTLWTGPSTLLRTGLADAQEIVF
                     VKSLEEAKQAIQEARDFEADIYAPEEFALALNYLAAAKDEAKIFRSEAQTKQDTHLFS
                     ARKSGEAVSLLAEKAQYQAKVAGAKAIEVKSDREITAIKAQVADTFNTDVNRQFTPLR
                     EKLMGELGAKEAVRKEARRARAQAESVLHTLTLEERAAPAPQ"
     gene            1899..2453
                     /gene="folE"
                     /locus_tag="VN444_00940"
     CDS             1899..2453
                     /gene="folE"
                     /locus_tag="VN444_00940"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWR20406.1"
                     /translation="MIERLVRDLLKEIGEDPDREGLVKTPERVERMFTFLTSGYGKQV
                     DDVLNKAVFQDNYDEIVVVKDIDFFSLCEHHLLPFFGKCHVGYLPDGKIVGLSKLVRL
                     VEMYSRRLQVQERLTSQIANALLEALQPRGVAVVMEAQHLCMMMRGVEKQNSKAVTSS
                     MHGIFRERIESRNEFMQLIRSHAT"
     gene            2610..>3114
                     /locus_tag="VN444_00945"
     CDS             2610..>3114
                     /locus_tag="VN444_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HWR20407.1"
                     /translation="MGVHDHHTEAKKVGPIRYVRLSITDSRKAEDDHSGRLIESLLNE
                     AGHCQIASMILKNDPKGLREVMKKLCEEEIDLIVMTGGTGLSKKDQTIEAISPVLDKV
                     LPGFGELFRSLSFLEVGSAALMSRALCGTAKGKVIVSLPGSEDAVRLALTKLLIPEVQ
                     HLVWQASR"
BASE COUNT          706 a          785 c         1000 g          623 t
ORIGIN      
        1 aggcccgcaa ccatggagcg cgcgtcctcg gcttctatga tgggttcaag tggcttgcgc
       61 gaggcgacac gagtcatatg gtagagatcg agatcgacga ggtgtcccga atccatttcg
      121 aagggggttc tatccttcga acgtccaggg agaatccggc caaatccccg gatacgctcc
      181 gaaatgtagc tgaggcactt gataaactgg aggtgtcgtg cctgttgacg atcgggggtg
      241 atgacaccgc tttcgcggcg tgtcggctgt cggaagccgt gaaggggcga gtcgccgtcg
      301 cgcatgtgcc gaagacgatc gacaacgatc tcccgctccc gcaggaggtg acaacgtttg
      361 gctttacgac ggcctgcaac ctcggcaaag atatcgtcca gaacctgatg gaggatgcgg
      421 tgacgacgaa ccggtggttc ttcgtcacag taatgggccg acgggcaggt catctggcgc
      481 ttggcatcgg aggcgctgcc ggcgccacat taacggtaat cggagaggag tttccagaac
      541 caagaatccc ccttcagacg ctggttgaca ttctggaagg cgcgatcatt aagcggcgcg
      601 cctcagggaa ggggcatggc gtggcgattc tgagcgaagg gctggctgat aaacttgatc
      661 ccgccgattt tggctcggtt gaacgggacg gttatgggaa tgtccgcctc tcggagcttg
      721 tgttggtgca agtactgaaa gagcgggtca ctgaaagcct tcgggcgcga ggcgtcgatg
      781 tgacgatcat agccaaggat cttggctatg aactccgctg cgctccaccc ggcgcactgg
      841 atatccagta ttgtcgcagt cttggctatt gggccacccg ttttctattg gacggtcaca
      901 cggaggccat ggtgaccatc caggggggga agatggtccc gatcccgttt cgagagatgt
      961 tggatcatcg gaccgggaag atccgtgtcc ggtacgtcga tatcgactcc gagtcgtatc
     1021 agacgctccg agcctatatg atccgcctgg aggttcagga ttttgagacg ccagggcaga
     1081 tcgagacgct cgcgcatggc ggacacctcg agcaggctga attcgtgagc cggtttggtt
     1141 atctcggcga tcgacgatct tagaagctgc agaaaccaag gggagcgtgt ttctcgcgaa
     1201 ggagtcagca caatgagata ctggaggcga agcattcagc gcatgatctt cggtgtggtg
     1261 gtcgtactgg ttccgtgtac gctctggaca ggcccttcga ctttgctcag gacaggcttg
     1321 gctgatgcgc aagagatcgt cttcgtaaag tcgctagagg aggcgaaaca agccatccag
     1381 gaggcgagag acttcgaggc ggatatctat gctccggagg agttcgccct tgccctgaac
     1441 tatttggcgg cggccaagga tgaggcaaaa atcttcagat ctgaggcaca gacgaagcag
     1501 gatactcacc tgttcagcgc caggaagtcg ggggaggcgg tcagcctgct cgcggagaag
     1561 gcacagtacc aggcgaaggt ggctggggca aaggcgatag aggtgaagtc cgatcgagag
     1621 atcaccgcca tcaaagcgca ggtcgccgat accttcaaca cggatgtcaa ccgacagttt
     1681 accccgttgc gagagaagtt aatgggcgaa ctgggcgcaa aggaagctgt aaggaaagag
     1741 gcccgcaggg cgcgggcgca ggccgagtcg gtgctacaca cattgacgct cgaagagcgg
     1801 gccgcgccgg caccacagta ggaattttac caggaaccta aaagcattcg cagttgagcg
     1861 tcaaaggtgc cggtgttacc cacacggaga ataggagaat gatcgagcgg ttggtcaggg
     1921 atctgctgaa ggagatcggc gaggatcccg atcgggaagg attggtcaag accccggagc
     1981 gggtagagcg gatgttcacc ttcctcacat ccggatatgg caaacaggtg gatgatgtcc
     2041 tgaacaaggc ggttttccag gataattatg atgagatcgt cgtggttaag gatatcgact
     2101 ttttttcctt atgtgagcat catctgctcc catttttcgg taagtgccac gtcggctatc
     2161 taccggacgg caagattgtc ggattgagca agctggttcg actggtggag atgtacagtc
     2221 gccgactcca ggtgcaggag cgtctgacct cccagatcgc taacgccctt ctggaggccc
     2281 tgcaaccacg cggggtggcc gtcgtcatgg aagcccaaca cctctgcatg atgatgcgtg
     2341 gcgtggagaa acagaatagc aaggccgtga cctcgtcgat gcacgggatc ttccgcgagc
     2401 ggatcgagag ccgaaacgag ttcatgcagt tgattcgctc gcacgcaaca taaagaaact
     2461 gatctctctc ctggagcaag ggaatacggt gacggaacgc gacctgttgc cgctgttcac
     2521 attgaccgga atcgattatt ccactactat tatgagttaa gcgggagctg tcccgggctg
     2581 aacagagcct tgatctcacc gaggaaccca tgggcgttca cgatcaccat acagaggcga
     2641 agaaggttgg gccgatccga tacgtgaggc tcagtatcac cgatagtcgc aaggcggaag
     2701 acgaccactc aggccgactc attgaatcgc tgctgaatga ggccggacat tgccaaatcg
     2761 cctcaatgat cctgaagaac gatcccaaag gcctgaggga ggtcatgaag aagctgtgtg
     2821 aagaggagat tgatctgatc gtcatgaccg gggggacggg gctcagtaag aaggatcaga
     2881 cgattgaagc gatcagcccc gtgttggaca aggtgctgcc gggctttggc gagctgttcc
     2941 ggagcctgag cttcctggaa gtcgggagcg ccgccttgat gagccgcgcc ctgtgcggga
     3001 cagcgaaagg gaaggtgatt gtgtccctcc cgggttcgga ggacgcagtc cgtctcgccc
     3061 tgacaaagct cctgataccg gaggtgcagc acctcgtctg gcaggcgtct cggt
//
LOCUS       DATMXI010000046         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_19817, whole genome shotgun sequence.
ACCESSION   DATMXI010000046 DATMXI010000000
VERSION     DATMXI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19817"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..2035
                     /gene="gspE"
                     /locus_tag="VN444_00950"
     CDS             314..2035
                     /gene="gspE"
                     /locus_tag="VN444_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HWR20408.1"
                     /translation="MGSGQWSGVREPLGEILIREGLITRDQLQRGLARQRELGKRLGE
                     TLVELGYVSEEDIAKALAEQFSLPYLSLASLSITPVPIHHRLSSKYLREHKVFPIEIK
                     DGALTVAMSDLTDPCTLDDLRLSTGLTITVYLAQDREILDAIDQYYGEGVATIEKIVK
                     GYSEEDGGASGEDREDIDHLRDLASEAPVIQLVNLLITRAVEARASDIHIEPFEDTLR
                     IRYRVDGVLVDHESPPKRFQRAVISRVKIMAKMNIAERRLPQDGRIRLQILGKDLDLR
                     VSTIPTLYGESVVMRILDRSSLLVTLGELGMTEDVRLGFQRLIRKPHGMILVTGPTGS
                     GKTTTLYTALSEINSADKKIITIEDPVEYQLQGVNQIHVKPKIGLTFANGLRSIVRQD
                     PDVIMVGEIRDAETADIAIHSALTGHLVFSTLHTNDAPGAITRLLDMGIESYLVSSVL
                     AAVLAQRLVRVICPECREPYQLDGAAVRKMGIKTEVAHVLQVFRGKGCAACNFTGYHG
                     RIGIYEFLVIGEEVQRMILEKADANMIRQKALQLGMKTLWEDGWRNVELGVTTLDDLL
                     RVTKEEA"
     gene            2481..>2823
                     /locus_tag="VN444_00955"
     CDS             2481..>2823
                     /locus_tag="VN444_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspF"
                     /protein_id="HWR20409.1"
                     /translation="MGTFQYTATDRTAKIIHGSMEASDERAVVTWLQANGYYPIKIGQ
                     AGATVEAKPGLARVQIRFARGPSAQEVLAFTQQLATLLEAGMELDRSLSILLDLTDNQ
                     RFRSILRGVLAD"
BASE COUNT          620 a          760 c          841 g          602 t
ORIGIN      
        1 cctctgcagc gctcaacagg gatggcggcc cccaggagac catgagggta tcccaacgga
       61 gatccactgt ggcatttatt tcgttgaggg tagatacccc gcggcttgcc gcagggtagt
      121 tcatttctca ggtgaaaccc caaactgtta agcgaattcg atctagactg ttggtagcag
      181 gcggcgttaa gtccttgaca cgatctttcg agccgatcta tcatccgact agctcaatgg
      241 cttgttttcc gggagttgaa gtagcggatt cagtcgcgta aaagatgccg tagcatcgag
      301 gatggatcac acgatgggca gtgggcagtg gagtggggtg cgcgaaccgc ttggcgaaat
      361 cctgatccgt gagggcttga tcacccgaga tcagctccag cgaggacttg cccgccagcg
      421 ggagcttggc aaacgattgg gcgagaccct tgtggagctc gggtacgtct cggaagagga
      481 cattgcgaag gccctcgcag agcaattctc gcttccctac ctgtccctcg cctccctctc
      541 gattacaccg gttccgattc atcatcgtct ctcatcaaag tacctgcggg aacacaaggt
      601 cttcccgatt gagatcaaag atggcgcgtt gaccgtcgcc atgagcgacc tgactgatcc
      661 gtgtacgctg gacgaccttc ggctgagcac cggtctcact atcacagtct atttagccca
      721 ggaccgggag attctcgatg cgatcgatca gtattacgga gaaggcgtgg caacgatcga
      781 gaagatcgtc aaggggtaca gcgaagagga tggcggggct tccggagagg atcgcgagga
      841 catcgatcac ctgcgcgatc tggcctccga ggcccctgtt atccaacttg tgaacctcct
      901 gatcacccgg gccgtggagg cccgcgccag cgatatccat atcgagccgt tcgaggatac
      961 gcttcggatc cgctaccggg tggatggcgt gctggtagac cacgagtccc ctcccaagcg
     1021 gttccagagg gccgtgatct cccgtgtcaa gatcatggcc aagatgaaca tcgccgagcg
     1081 acgcctccct caggacggcc ggatcaggct tcagatcctg gggaaggacc tggacctccg
     1141 ggtctccacc atcccgacct tgtacggcga aagcgtagta atgcgaatcc tggatcgcag
     1201 tagcctgctc gtaaccctgg gtgaactggg catgacagag gacgttcgtc tggggtttca
     1261 gcgcctgatc cgaaagccgc atggcatgat cctggtgacc ggcccgacgg gaagcggcaa
     1321 gaccactacc ctgtacaccg cattgagcga gatcaactca gccgacaaaa agatcatcac
     1381 tatcgaggat ccggtggagt atcagcttca gggggtcaat cagattcacg tcaagcccaa
     1441 gattggcctg acctttgcca atggcctgcg ctctatcgtc cgccaggatc ccgacgttat
     1501 catggtcggg gagattcggg atgcagaaac ggcggacatc gccatccatt cagccctcac
     1561 aggacacctc gttttcagta ccctgcacac gaacgacgct cctggcgcca ttacccggct
     1621 gctggacatg gggatcgaga gctacctggt ctcgagtgtc ctggcggcgg tcctggctca
     1681 gcgcctggtc cgcgtcatct gccccgagtg tcgcgagccg tatcaattgg atggggctgc
     1741 agtcagaaag atggggatca agaccgaggt tgctcacgtg ctgcaggtgt ttcgtggcaa
     1801 ggggtgcgct gcttgtaact ttaccgggta ccatggccgc ataggcatct acgagtttct
     1861 ggtgatcggc gaggaggttc agcggatgat cctggagaag gctgacgcca atatgatccg
     1921 gcagaaggcg ctccagcttg gcatgaagac gctttgggag gatggctggc ggaatgtcga
     1981 gttaggggtc acgaccctgg atgacctgct tcgggttacc aaagaggagg cgtaagcagg
     2041 accatttcct tatgtaaagt tgacaaccct ttgtcaataa agcagagaga gggtccttgt
     2101 ctgctttatt ttcgaggcca gttccaacga cctacagggc cattgcaaga cctaatttct
     2161 gttgacagtg catcggtagt tgttatagtc ctaaacttat acgtacgatt cggtgtcaat
     2221 aaaggtaaaa cggaactata aggtagatag actgaaggct gttagggcaa agcagtgatc
     2281 gcgcaacact tcgggcagtt gagttggccg atgaagtacg caatgtatca aagcggttga
     2341 gcttcttgcc caatgaggag tcctctctga gagaaccaaa ggtcgtagaa gccgggaagt
     2401 tcttgaatgt gccgtgtgag ataatgtata gccttcagcc ttcagcctca acacctaagt
     2461 ggttacagta aaacctgtgc atggggacat ttcaatacac cgccactgat cgcaccgcca
     2521 agattatcca tgggagcatg gaggcttccg acgaacgggc ggtcgtgacc tggctccagg
     2581 ccaatggcta ctacccgatc aagattggtc aggccggtgc gacggtggag gccaagcctg
     2641 gtctcgctcg ggtccagatt cgatttgcga ggggaccgtc cgcccaggag gtcctggcct
     2701 tcacgcagca gctcgccaca ttgctcgagg ctgggatgga gttggaccgg agtctgtcga
     2761 tcctgctgga cctgaccgac aatcagcggt tccggtcgat tctccgtggc gttctggccg
     2821 aca
//
LOCUS       DATMXI010000047         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_277756, whole genome shotgun sequence.
ACCESSION   DATMXI010000047 DATMXI010000000
VERSION     DATMXI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277756"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..703
                     /locus_tag="VN444_00960"
     CDS             191..703
                     /locus_tag="VN444_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWR20410.1"
                     /translation="MTAIRVHPDNRRIEAPAEMVRFLDEEGLVYRNWDVSKLRGGLRD
                     NYALTEAEKAEILATFEGDLEKLKAEGGYVAADIVVLWEKTPDLEVLLDKFNREHHHT
                     EDEVRFVVDGHGIFTIRGCSGRCFDVGVGPGDLIAVPAGTRHWFTLAEDRRIKCIRLF
                     HDPAGWAAIY"
     gene            803..1114
                     /locus_tag="VN444_00965"
     CDS             803..1114
                     /locus_tag="VN444_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20411.1"
                     /translation="MMSEEAKFTIEVICPYCQARLIVDPERGAVLHHELPPKEAAVTD
                     LKAAVEGLKGEAGRREARFKESMEAEKEKGKLLERKFKELLKKAKDEPVARPIRDIDL
                     E"
     gene            1282..>2200
                     /locus_tag="VN444_00970"
     CDS             1282..>2200
                     /locus_tag="VN444_00970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWR20412.1"
                     /translation="MVSPAEPPTAMNDHDLLCTLLKRVSRSFYLTLKVVPSDVRRPIG
                     LAYLFARAADTIADTTLIGRADRLKHLDLFREAFREGRVERILAIKEVLAERQQVAAE
                     RELLTRLDEGFAILHSLSLSDQTMIRDVVLTLTEGMVMDLATFPGEDEGRLVALQARA
                     DLDRYTYYVAGCVGEFWTDIHMVHRSSLAGWDREAMRRRGVRFGKGLQMTNVLRDLPK
                     DLRIGRCYLPRQELEALGLQPADLLDPTAVVKVKPLLHSLLALTLEHYQEGWAYTLQI
                     PRHEVRMRLACIWPLFIGLKTLALLERSPN"
BASE COUNT          449 a          636 c          698 g          417 t
ORIGIN      
        1 gactatatgt ctggggagcg aatcccttcc aggccaagcg gcacgtggaa gccttcgagt
       61 ttatgttcga atacctgctc cgtcggtgga ctgtaggggc aggccttgtg cctgcccaga
      121 agggcgacca caagggtcgc ccctacgagc tggccgaggc agggtaaggg ggcatcatgg
      181 gagggaaagc atgacggcga tcagggtgca ccccgataac cggcgcatcg aggcgccggc
      241 agagatggtg aggtttctcg atgaggaggg cctcgtctac cgcaactggg atgtgtcgaa
      301 actgcgaggt ggattgcggg acaactacgc cctgaccgag gccgaaaagg cggagatcct
      361 ggcgacgttt gagggcgacc tggagaagct caaggctgaa gggggatacg tggcagccga
      421 catcgttgtc ctctgggaaa agacccccga cctggaagtg ttgctcgaca agttcaatcg
      481 cgagcatcac cacactgagg acgaggtgcg gttcgtggtc gatggccacg gcatcttcac
      541 gatccgcggc tgcagtggtc gctgcttcga tgtaggggta ggcccaggcg atctgatcgc
      601 cgtgcccgct gggactcgtc actggtttac tctggccgaa gatcggcgga tcaagtgcat
      661 tcgcctcttc catgaccctg ccggatgggc ggccatttac tgacccgaga gaccctcttc
      721 cccagtaccc ataccgaccc ggttttgcaa cgggatgtac ggtatgctag tctgtctttg
      781 tgcgaggccg ggttgggatc gaatgatgag cgaagaggcg aagtttacca tcgaggtgat
      841 ctgcccttac tgccaggcga ggctgatcgt tgaccccgaa cgtggcgcag tcttgcatca
      901 tgagctgcca cccaaagagg cggccgtcac agacctgaag gctgcggtcg aggggttgaa
      961 gggcgaggct ggccggcggg aggcgcgctt taaagagtcg atggaagcag agaaagaaaa
     1021 agggaagctg ttagagcgga aattcaagga gctgctcaag aaggcgaaag acgaacccgt
     1081 cgcccggccc attcgggaca tcgacctgga gtagcactac tcaggtgttt gggctgaagg
     1141 ctctagatca tctcacaagg cacgaatcga gcccctcagg accgtgtcgg agtcttgtat
     1201 gatcacacat gacgaacccc taacagcctt cagtcttcag tctatcgacc tgagtatagt
     1261 tccctggatt agcccccacc tgtggtgagt ccagccgaac cgcccaccgc gatgaacgat
     1321 cacgacctgc tctgcacgct cctcaagcga gtcagtcgct cattctattt gaccctgaag
     1381 gtcgttccca gcgatgtgcg gcgaccgatc ggtctggcgt acctcttcgc cagggcggca
     1441 gacactatcg ccgacaccac tctcattggc cgcgcagacc gcctgaagca tctcgatctc
     1501 ttccgcgagg cgttcaggga aggacgggtg gagcgaattc tggcgatcaa agaggtgctc
     1561 gcggaacggc agcaggtcgc ggcggagcgg gaactgctca cccgcctcga cgagggattt
     1621 gccattcttc attccctcag tctgtccgat cagacgatga tccgtgacgt agtactgacc
     1681 ctgacggaag ggatggttat ggatctcgcc accttccccg gagaggatga gggtcgcctc
     1741 gtcgccctgc aggcgcgagc cgacctggac cgttacacct actatgtcgc cgggtgcgtg
     1801 ggggagttct ggaccgacat ccacatggtg catcgctcgt ccctcgccgg gtgggaccgg
     1861 gaggcgatga ggcgacgcgg cgttcgattt gggaaggggc tccagatgac gaacgtgctg
     1921 cgcgatctcc cgaaggacct ccggatagga cgctgttacc tgccgcggca ggagctggaa
     1981 gcgctgggcc ttcagccggc cgacctgctc gacccaaccg ccgtggtgaa ggtcaaaccg
     2041 ctgctacact ccctcctcgc ccttacactc gagcactacc aggaaggctg ggcctatacg
     2101 cttcagattc cgagacacga agtccgaatg cgtctggcct gcatctggcc gctgttcata
     2161 ggcctcaaga cactggcact gctcgagcga tcgcccaatc
//
LOCUS       DATMXI010000048         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_168225, whole genome shotgun sequence.
ACCESSION   DATMXI010000048 DATMXI010000000
VERSION     DATMXI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168225"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VN444_00975"
     CDS             <1..1007
                     /locus_tag="VN444_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20413.1"
                     /translation="YQAAQVIEGAAERCAQLANRLLGFARGGKHLEIPVDLHTAVREV
                     VDLLSRTIDKRITITQRLCAEQAVALGDPSQIQQVILNLAVNARDAMPHGGNLHFESD
                     VVTLDDDYCRRNLGATAGDYVMLSVTDTGMGIPKEIQGRVFEPFFTTKEPGKGSGMGL
                     AMVYGIVKNHGGTIRVYSEEGSGTSFKVYWPLATGVVPAPVSVEQTPPILGQGRILLV
                     DDEEVVRTTSAELLRHIGYEVVTASDGQEAVDYYRQFGRQIDLIIIDMVMPRLSGADC
                     FRALKALEPNVKAILTTGFGLNEAAQRLLDEGMAGFLQKPYLINELSEVVARVLSREA
                     "
     gene            1053..1667
                     /locus_tag="VN444_00980"
     CDS             1053..1667
                     /locus_tag="VN444_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWR20414.1"
                     /translation="MDAIDAEVTETASNRLDLDRWSSIGLVICVGFVLSFIVFLMVCA
                     WEARRIESELERKADAMTHDIMHGVKSGLGTIRAISSIYATAEVVDPMWVRTEAERML
                     LGQDTIRTVAWAPKVADAGREDYESAIRGRFGYRDFEIRERDLQGHVISAGRRAIYFP
                     VSDSLPMGGALARFRLASIWLPTNCSTTRSGPPGRPGSWWLRLR"
     gene            1604..>3182
                     /locus_tag="VN444_00985"
     CDS             1604..>3182
                     /locus_tag="VN444_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWR20415.1"
                     /translation="MLNHALRTAWKTGELVVTPPVKITPGDARESRVVVFAPVYRLKT
                     PSEAFEQSPQNMKGILVGIIKVEDLIEEVLKEWNGEGIEIWVYDRMEEGNLRLLAAPE
                     SREERARGEDPLALGSLGSNRLRSVTTMAVADRRWELQFVGTDQYLVTAKLRSRWEVL
                     VLGLVFTMLVAGYLSAKRRQTAEVQRLVSIRTAELNRVNRELAAEIQERARVAEAFRE
                     SEARFRVMADTAPVMIWMTGLDGRGTFYNKTLLEFTGRSREEELTDWIAGVHPDDRPR
                     LENYWKTFALREPFEIEYRQRRADGVQRWTLDRGMPRFLPDGAFAGYIGCGIDVTELK
                     EAQQAMRQAHDAAIESLRLKSEFLANMSHEIRTPMNGILGMTDLALRTALSVEQREYL
                     TLTKSSGESLLEIINDILDFSKIEAGRLDLEQAPFLLRESMSMAIKPLALRAHSKRIE
                     LSCHVAHDVPEALIGDPVRLRQIVVNLIGNAIKFTERGEIVLRITQEHEGPTGVLTVG
                     NGAPVCRLHCTVTDTGIG"
BASE COUNT          632 a          839 c         1058 g          653 t
ORIGIN      
        1 tctatcaggc ggcgcaggtg atcgagggag cggcggaacg ctgcgcgcaa ctggcgaatc
       61 gcctcctggg attcgcccgt ggcggcaaac atctggagat acctgtggac ctgcacaccg
      121 cagtgcggga ggtggtcgat ctgttgagcc ggacgatcga caagcggatc accatcacgc
      181 agcgtctgtg cgccgagcag gctgttgcgc ttggcgatcc cagccagatc cagcaggtaa
      241 tccttaatct tgccgtgaat gcccgggacg ccatgccgca cgggggcaac ctgcacttcg
      301 agtcggatgt cgtcacactc gatgacgact actgtcgtcg caacctcggg gcgaccgcag
      361 gggactacgt catgctgtcg gtcacagaca ccggcatggg cattcctaaa gagatccaag
      421 ggcgcgtttt cgagccgttc tttacgacca aggagccagg caaggggtca ggaatggggc
      481 tggccatggt ctacggtatt gtcaagaacc atggcggcac catccgcgtc tacagcgagg
      541 aggggtccgg cacctcgttc aaggtctatt ggcctcttgc gacgggtgtc gttcctgcgc
      601 ctgtcagcgt cgaacaaacg ccaccgattc tcggccaggg ccgcatcctc ctcgtagatg
      661 atgaggaggt tgtgcgcacc acatcagcgg agttgctgcg ccacataggc tatgaagtgg
      721 tcactgcttc ggatggtcaa gaggccgtcg attactaccg gcagttcggt cgccagatcg
      781 atctcattat cattgacatg gttatgccgc ggctttcagg cgccgactgc ttccgagcgc
      841 tgaaagcgct cgagccgaat gtcaaggcta tcctgactac gggttttggt ctgaacgagg
      901 ccgcccagcg gcttctcgat gaagggatgg cgggcttcct ccagaaaccg tacctgatca
      961 acgagctttc agaggtggtg gccagggttc tgagtcgtga ggcgtgaggg gccgctggat
     1021 agtaaattga cgcgataggc gcaacagacg aaatggacgc aatagacgca gaagtaacag
     1081 aaaccgcatc caacaggttg gaccttgatc gatggagctc gatagggctg gtcatctgtg
     1141 tcggttttgt tctctctttt atcgtgtttc ttatggtctg cgcgtgggag gcgagacgta
     1201 ttgagagcga gctagagcgc aaggcagacg ccatgacgca cgacatcatg catggcgtta
     1261 agagcggtct tggcaccatt cgtgcgatca gcagcatcta tgccaccgcc gaggtcgtcg
     1321 atccaatgtg ggtgcgcacg gaagccgaga ggatgctatt gggtcaagat accattcgta
     1381 ccgttgcctg ggcgccgaag gttgctgacg ctggacggga ggattatgag tcggcgattc
     1441 gaggccgttt cggatatcgg gatttcgaga tcagggaacg ggacttacag gggcacgtca
     1501 taagtgctgg ccggagggcg atctatttcc cggtttcgga ctccctcccg atgggggggg
     1561 ctttggcgag gttccggctg gcctcgatct ggcttccgac aaactgctca accacgcgct
     1621 ccggaccgcc tggaagaccg gggagctggt ggttacgcct ccggtgaaga tcacgcctgg
     1681 cgacgcgcga gagtcacggg tcgtggtgtt cgcccctgtg tacaggctca aaaccccttc
     1741 cgaggcgttc gagcagtctc ctcagaatat gaaggggatc ctggtcggga tcattaaggt
     1801 cgaggatctc atagaggaag ttcttaagga atggaatggg gaggggatcg agatttgggt
     1861 ttatgaccgg atggaggagg ggaatctgcg tctgctggca gcaccggaga gtcgtgagga
     1921 gcgtgcccgc ggagaggatc cgttagcgct gggttcgctc ggatcgaata ggctgcgatc
     1981 agtcacgact atggcggttg cggatcggcg ctgggagctg cagtttgttg gtaccgacca
     2041 gtacctggtc accgcgaagt tgcggagccg gtgggaggtc ttggtgttgg gcctcgtctt
     2101 cacgatgctt gtggctggtt acttgtctgc gaagaggcgt cagaccgcag aggtgcagcg
     2161 gctcgtgtcg attcggacgg ctgagctcaa ccgggtcaac cgagagttgg cagccgaaat
     2221 ccaggaacgg gcgcgggttg ctgaggcgtt ccgggaaagt gaggcgaggt tccgagtcat
     2281 ggccgatacg gcgccggtca tgatctggat gaccggactg gatggacgcg gcaccttcta
     2341 caataagacg ctgttggagt ttaccgggcg ctcgcgggaa gaggaactga cggactggat
     2401 agccggggtt catcctgacg atcggccgcg gctcgagaac tactggaaga cattcgcgct
     2461 gcgtgaaccg ttcgagatcg agtatcgcca gcgacgggcc gatggcgtcc aacgctggac
     2521 cctggatcgg gggatgcccc gcttcctgcc tgatggcgcg tttgccggat atataggctg
     2581 cgggatcgat gttactgagc tgaaggaggc gcagcaggcc atgcggcagg ctcatgatgc
     2641 cgcgatcgag tccttgcgcc tcaagtccga gttcctggcc aatatgtcgc acgagatccg
     2701 gacgccgatg aacgggattc ttgggatgac ggatcttgcg cttcggactg ctctcagcgt
     2761 tgagcagcgc gagtacctca cactgacgaa aagctccggt gagtccctgc tcgagatcat
     2821 caacgatatc ctcgatttct cgaagatcga ggcaggccgt ctcgatctgg agcaggcgcc
     2881 gttcctgctc cgcgagtcga tgagtatggc gatcaaaccc ctggcgcttc gggcccattc
     2941 aaaaaggatt gagctcagtt gtcacgtcgc acacgatgtt ccggaggctc tgatcgggga
     3001 tccggttcga ttgcgacaga tcgtggtcaa cctgatcggc aacgccatta aatttaccga
     3061 acggggcgag atcgtgttgc gcataacgca ggagcacgaa ggtccgacag gcgttctgac
     3121 cgtcggcaac ggcgctccgg tatgccgcct ccattgtacg gtgaccgaca ccgggatcgg
     3181 ga
//
LOCUS       DATMXI010000049         5769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_301283, whole genome shotgun sequence.
ACCESSION   DATMXI010000049 DATMXI010000000
VERSION     DATMXI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301283"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VN444_00990"
     CDS             complement(<1..1622)
                     /locus_tag="VN444_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWR20416.1"
                     /translation="MRITVRLVLSILVVVSAFVLAFTLVQVRQEKQRQRVDLERRALL
                     LAESLGESIEPLVQQGHSARLHRIVEKFGNRERIAGVAVYDAKGLPLAVTQTFSAQFT
                     TPPEIVMEAITADQTMNDLITVGNKQMFVFVLPLRVEGSEEQVAGALVLFHDATYIQD
                     RLQQIWQHNFLRLLVQALLISLVSLVVVRWTLIRPMQQMTEWMKRLRMGDAAGPSPPS
                     HETLLHPLATEVTHLANSLSAARATAEHEARLRRAGDSLWTAQRLKEHLRTQFQGRSL
                     FVVSNREPYSHVKRGRRIECVVPASGLVTALEPVLAACEGTWIAHGSGDADSLVVDHN
                     DRLRVPHERPQYTLKRVWLTKEEEEGYYYGFANEGLWPLCHIAHTRPIFRAEDWRYYQ
                     LANEKFAQATLAELEGVDEPCVLIQDYHLALLPGLIKKQRPDARVGLFWHIPWPNPES
                     FGICPWQCELLHGMLGADLIGFHIQFHCNNFLETVDRVLESRIEWEHFAVHRGDHRTC
                     VKPFPISIAYSDAPHASAGTTGEEKCKEELLT"
     gene            complement(1724..2932)
                     /locus_tag="VN444_00995"
     CDS             complement(1724..2932)
                     /locus_tag="VN444_00995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20417.1"
                     /translation="MNVIGLDDYQEVTPKGTVQFLRSLAKRVRGRRFLHINSTRYGGG
                     VAEILHRLVPIMTELGVDARWETIKGDPAFFSVTKALHNALQGREALITKEMRGHHAA
                     INRENARHLSLEGDLVLIHDPQPAPLIDYRPAEGKWVWRCHIDLSHPQRSAWRLLRKY
                     VAKYDAVIFSLPKFSQQLPIPQFLVCPSIDPLSEKNRDLSQAEVDGILARLDIPRDKP
                     ILLQVSRFDRFKDPIGVIKAYRLVKKYHDCRLILAGGRAQDDPEGEEVLADVREEAGL
                     DPDIHVLELPPDAHLEINALQRAATIVLQKSLREGFGLTVAEAMWKGKPVVGGAAGGI
                     TVQVIDDVTGYTVNSVEGAAFRVRYLLNNPDLMAKMGEAGREYIRRDFLITRHLADYL
                     AMLMILTGNE"
     gene            complement(2929..3600)
                     /locus_tag="VN444_01000"
     CDS             complement(2929..3600)
                     /locus_tag="VN444_01000"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HWR20418.1"
                     /translation="MLERSEVPFEFFYCIELRQVLGLKAETEKELARLLRQVSLDSVY
                     YHTYGLLLRHRYRAGAYGSDFATWAATQVRDRVLGERLAAVDPLRYNSLADLREAIVC
                     VIDNHLESITVVPRVVYSEPFEFIESKIVGVPTGLEAYTLEEFMQALSETDDTTIYYH
                     AIEARRRLKHTRTDFSAWLRDDLHLPVLAARVQALDPYAGGLERMRTHILAYCRQVLT
                     LGRDL"
     gene            complement(3690..3857)
                     /locus_tag="VN444_01005"
     CDS             complement(3690..3857)
                     /locus_tag="VN444_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20419.1"
                     /translation="MKLELGQAGYVADIGWRTTRIRMLPNNMVKEGIDIPFPQRVVHI
                     HAMDGDAPLSR"
     gene            complement(3951..4241)
                     /locus_tag="VN444_01010"
     CDS             complement(3951..4241)
                     /locus_tag="VN444_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20420.1"
                     /translation="MTKKAVVTAAALVAILVVLALSTGVAFAATRTITGEVISAELSA
                     KTLMIKAQGQEMSFSVADRAATAMADLKPGDQVTVNYTEAEGKRIAQSITKG"
     gene            4670..>5769
                     /locus_tag="VN444_01015"
     CDS             4670..>5769
                     /locus_tag="VN444_01015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWR20421.1"
                     /translation="MTEQTSVISASTPVTGLAGLNAAQQAAVTHGDGPLLIIAGAGTG
                     KTTVIAHRIAHLINSRRARPEQVLALTFTEKAAAEMERRVDLLVPYGFTDTWISTFHA
                     FGDRVLREHALVLGLSPDFRLLTVPEQVIFVQEHLFELPLDYFRPLGSPLRHVQALLQ
                     LFSRAKDEDVGPEKYASYAEELADRATASPDDKALAEQGEREIEIARTYRHYQELMAR
                     EGKLDFGDLMTQTLNLLREHPLVLQRLQERFRYILVDEFQDTNYAQFQIVKLLTAAHR
                     NVTVVGDDDQSIFKFRGASISNILSFRREFPETETVVLLENYRSTPNVLDAAYRLIQH
                     NNPDRLEYQEGVDKRLQPCNGDGPAVESVVFQT"
BASE COUNT         1182 a         1708 c         1629 g         1250 t
ORIGIN      
        1 tctgtgagca actcctcttt acacttttct tcgcctgtag ttcctgctga tgcgtggggg
       61 gcatccgaat aggcgatgct gattgggaac ggcttgacac aagtcctgtg atcgccgcga
      121 tggaccgcga agtgctccca ctcgatgcgg gattccaaca ctcgatccac cgtctccagg
      181 aagttgttgc agtgaaactg gatgtggaac ccgatcagat ctgcacccag cattccatga
      241 agtaactcac attgccatgg gcagatgcca aacgattccg gattaggcca cgggatatgc
      301 caaaagagcc ctacgcgggc atcagggcgc tgtttcttga tcagcccagg tagcaaggca
      361 aggtggtagt cctgaatcag cacgcatggc tcatcaacgc cttcgagttc ggccaacgtg
      421 gcctgcgcaa acttttcgtt cgccagttga tagtaccgcc aatcctcggc tcgaaagatg
      481 ggacgggtgt gggcgatatg acagaggggc catagcccct cattggcaaa gccatagtag
      541 tacccttctt cttcctcctt tgtcagccac acccgcttca gggtatattg cggacgttcg
      601 tgggggaccc tgagtcgatc gttatgatcg acgaccagac tgtcagcgtc accactccca
      661 tgggcaatcc atgttccttc acaggcggcc agcacaggtt ccagggcggt gactagaccg
      721 ctggccggga ccacgcattc gatccgcctc ccccgtttca cgtggctgta gggctcacgg
      781 tttgaaacga cgaagagcga tcgtccttgg aattgtgttc gcaaatgttc cttcagtcgt
      841 tgcgcggtcc aaagggagtc ccctgcccgc ctgagccgcg cctcatgctc agcggtcgct
      901 ctggctgcgg agaggctgtt ggctaggtgg gttacttcgg tggccaatgg gtgcaagagg
      961 gtttcgtgtg aaggaggcga ggggccagcc gcatccccca tgcgcagccg cttcatccac
     1021 tccgtcattt gctgcatggg tcgaataagc gtccaacgga caacgaccaa gcttaccagc
     1081 gagatcaata gggcctgaac cagaagtcgg aggaaattgt gttgccagat ctgctgcaag
     1141 cgatcctgga tgtaggtggc atcgtggaag agaacgagtg cgccggcgac ctgctcttca
     1201 gatccctcaa cgcggagcgg caacacaaat acaaacatct gcttgttgcc gacagtgatc
     1261 agatcgttca tcgtctgatc ggcggtgata gcctccatca caatctctgg cggtgtcgtg
     1321 aactgggcgg agaaggtttg cgtgaccgcc agcgggagcc ccttagcgtc gtatacggcc
     1381 actccggcaa tccgctcccg gtttccgaac ttttcgacga tccgatgaag ccgcgctgaa
     1441 tggccttgct gcaccaaggg ttcaatactc tctcccaggc tttcagctaa cagcaaggcc
     1501 cgccgctcaa gatcgaccct ctgccgctgt ttttcctgcc gaacctgcac gagggtaaat
     1561 gccagcacga aggcactgac aacaaccaga atcgagagca caagccgcac ggtgattcgc
     1621 atacgccttt cctctcgcgc tggagtagtt catggagccc gaagttgaag gaagtcttca
     1681 gggtactcta agctcaccct ctccagccct ctcttcattc gcttcattcg ttcccagtga
     1741 ggatcatgag catggccagg tagtcggcca gatgacgcgt gattaggaag tcacgtcgaa
     1801 tgtattcccg ccccgcctcc cccatcttcg ccatgaggtc tggattgttg aggagatatc
     1861 tgacacggaa cgctgcaccc tcgaccgagt tcacggtgta gccggtcaca tcgtctatca
     1921 cctggacagt gatcccgcca gccgcaccgc ccacgacggg cttgcccttc cacatcgcct
     1981 cggccaccgt gagaccaaac ccttcccgga gcgacttttg caggacaatc gtagcagctc
     2041 gctgaagtgc attgatctcc aggtgagcat cgggtggaag ctcaaggaca tggatatctg
     2101 gatcgaggcc tgcctcttcc cgtacgtccg ccagcacctc ttctccctcc ggatcatcct
     2161 gggctcttcc ccccgccagg atcagtcgac agtcgtggta tttcttaacc aggcggtaag
     2221 ccttgatcac cccgattggg tctttgaaac ggtcgaaccg ggatacctgc aggaggatag
     2281 gcttatccct cgggatgtcg agtcgcgcga gaatcccgtc cacctcagcc tgactgaggt
     2341 cacgattctt ctcgctgagc gggtcaatag acgggcacac aaggaactgc gggatcggga
     2401 gctgttgcga gaacttcggt aacgagaaga tgactgcatc gtatttcgcc acgtactttc
     2461 ggagaagccg ccacgcgctt cgctgcggat gcgacagatc gatatgacag cgccagaccc
     2521 acttcccttc tgctgggcga tagtcgatga gcggggcagg ttgaggatca tgtatcagca
     2581 ctaaatcccc ttcaagcgac aggtgtcgtg cgttttctcg atttatggcc gcatggtggc
     2641 cgcgcatctc ttttgtgatc aacgcttccc gcccctggag ggcgttatga agggcctttg
     2701 taacagagaa aaaggcggga tcccctttga tcgtttccca acgagcatcc acgccaagct
     2761 cggtcatgat cggtaccagt cgatgaagga tctcggctac cccgccacca tatctggtgg
     2821 agttgatgtg caggaaccgt cggcctcgca cacgctttgc gagagatcgg agaaactgca
     2881 cggtcccttt gggggtgacc tcttgatagt cgtcaaggcc tatgacattc ataggtcgcg
     2941 ccctagcgtc aacacctgcc tgcagtaagc caagatatgg gtccgcatcc gttccaggcc
     3001 cccggcgtaa ggatccaggg cctgtaccct ggcggccagt accggtaagt ggaggtcatc
     3061 ccgcagccag gccgaaaagt ccgttcgggt gtgcttcagc cgcctcctgg cctcgatggc
     3121 atggtagtag atagtcgtgt catctgtctc gctcagggcc tgcataaatt cctcaagggt
     3181 gtaagcctcg agtcctgtcg gtacccctac gattttggac tcgatgaatt cgaacggctc
     3241 gctgtacaca acacggggaa cgactgttat gctctccaag tgattgtcga tcacgcagac
     3301 gatcgcctcc ctgagatcgg ccagcgagtt gtaacgaagc ggatccaccg ccgcaaggcg
     3361 ctcgcccagg acccgatcac ggacctgggt cgccgcccac gtcgcgaagt cgctcccgta
     3421 cgccccagcc ctgtaccggt ggcggagcag caatccgtaa gtgtggtagt agaccgaatc
     3481 cagcgaaacc tgtcggagca gcctcgccag ctctttctcg gtttcggcct tcagccccaa
     3541 cacctggcgt agttcgatac aatagaaaaa ctcgaagggc acctcggaac gctcaagcat
     3601 tcaccccacc ccgggtcttc ccttcagcgc cttattaagt gccagggcgc cgtccagccc
     3661 aaacatctct cgtccttctt gtgcctgcct caacgggaaa gaggcgcgtc gccatccatt
     3721 gcgtgaatgt ggaccacccg ctgcgggaaa gggatatcga ttccttcctt aaccatgttg
     3781 tttggcaaca tccgaatccg cgtcgtccgc cagccgatat cggccacata ccccgcctgg
     3841 cccaactcca gcttcatata gtccccaacc tatatcctct cgcctctctc gcaagaagaa
     3901 aaggcgactc tcgatgcgag agccaccttt tctcggcctt tcgcgtcggc tcagcccttc
     3961 gtgatggact gagcgatacg cttcccttcg gcctcggtgt agttgaccgt cacctgatcg
     4021 cccggcttaa ggtcagccat ggctgttgcg gccctgtccg cgacgctaaa ggacatctct
     4081 tgcccctgag ctttgatcat cagggtcttc gcggagagtt ctgccgaaat cacctcaccg
     4141 gtgattgtcc tcgtcgcagc aaaggccaca ccggtggaga gggcgaggac caccaggatg
     4201 gcaacaagag ctgctgcggt tactaccgct ttcttggtca tgtcagcatc acctcctttc
     4261 atgcttgaac ttgactcgca gattccatac caaatcctgg aggctcaccg ctcatcgtga
     4321 tcaacctcct aattcttaag aaattgtttt ggtaacggtc taggcgagct ttagggcaat
     4381 tcaatcttct cttcacagat aagcatgcaa gatctaaaca gcagcttgta agagttacat
     4441 ggttcgggat tacggcccag attcttgtgt ctctttattc gcggcgaccc gtcacgaaca
     4501 ctacgatttc accggttact tcgttccctg ccccgtgtcg tgatttatgc ctgatggggg
     4561 cctctcgcgg catttggcgt gggtcagcgg ggatagcgct atggccggag ggagccacca
     4621 tgatattatg gtgtgacgtg gttgagcgac ctatgagggt tgacgaggaa tgacggaaca
     4681 gacctcagtg atctcagcga gcacgccagt gacgggcttg gcgggcctga atgcggccca
     4741 gcaggcggcg gtcacccacg gcgatggtcc gttgctcatc attgcgggcg ccgggacggg
     4801 gaagaccacg gtaatcgctc accggatcgc tcacctgatc aacagcaggc gggcccggcc
     4861 ggagcaggtg ctggcgctga cctttaccga gaaggcggcg gcggagatgg agcggcgcgt
     4921 ggacctgctg gtgccgtacg ggtttaccga tacatggatc tccaccttcc atgcttttgg
     4981 agaccgcgtc cttcgggagc atgccctggt gctggggttg agtccggact tccgcctgct
     5041 cacggtgccg gagcaggtca tcttcgttca ggagcatctg ttcgagcttc cattagacta
     5101 tttccggccg ctcggcagtc cgctgcggca tgtgcaggcg ctgctccaac tattcagtcg
     5161 agccaaggac gaggatgtcg gtcccgagaa atacgcgagt tacgcggagg aactggcaga
     5221 tcgagcgacg gcctctcccg atgataaggc gctggcagag cagggggagc gggagatcga
     5281 gatcgccagg acctaccgac attaccagga gctgatggcg cgagaaggga agttggattt
     5341 cggcgacctg atgacgcaga cactcaactt acttcgggaa catccgctgg tactccaacg
     5401 attgcaggag cggttccggt atatcctggt tgatgagttc caggatacca actacgccca
     5461 gtttcagatt gtgaagctgc tcacggccgc ccatcggaat gtgacggtgg tgggggatga
     5521 cgaccagtcg atcttcaagt ttcgaggggc ctcgatcagt aacatcctca gcttccgccg
     5581 ggagttcccc gagacggaga cggtcgtcct gttggagaac tatcgctcga cgccgaatgt
     5641 tcttgatgcc gcgtaccgtc tgatccagca caacaaccct gaccgactgg agtaccagga
     5701 aggcgtagac aagcgcctcc agccttgcaa tggtgacggc ccggccgttg agagtgtggt
     5761 cttccagac
//
LOCUS       DATMXI010000050         5837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_213817, whole genome shotgun sequence.
ACCESSION   DATMXI010000050 DATMXI010000000
VERSION     DATMXI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213817"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN444_01020"
     CDS             <1..521
                     /locus_tag="VN444_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20422.1"
                     /translation="RSGQGRLVGERTGGASAPERAVRLMISVITFSPTFFGQTVDRIA
                     LVRRVVLLALMLSVVPLLASQAAAQDDVGLPMHPKAILSSIVRQAGEGDGTRWIRVSF
                     TTNAPYKQVVKFYQEKVGGNAQISEVDSGKLLNTLILFSKDPTNQFSVNINSALGRKV
                     TEVELSRNFVHQ"
     gene            822..1031
                     /locus_tag="VN444_01025"
     CDS             822..1031
                     /locus_tag="VN444_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWR20423.1"
                     /translation="MERVTGTVKWFNDAKGYGFIAREDGDDVFVHYSAITGAGFRSLN
                     EGQAVEFEVVDGPKGKQAANVSRAA"
     gene            complement(1213..1725)
                     /locus_tag="VN444_01030"
     CDS             complement(1213..1725)
                     /locus_tag="VN444_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20424.1"
                     /translation="MRALLITLLGGWIVGTLLMSFVATQNFRTVDRLLSAPTVEFSRA
                     IAPLAHDEARGILRYVVSELNRLFFSAWGFTQLALGAVIVALALGLRPLDRTVITIAV
                     TVFVIVIVSLLLSQSLLSLGRSLDFVPRTLVSLELARFRRFHLIYTALDMLKLIFCVW
                     LLIRSARQAA"
     gene            complement(1779..2261)
                     /gene="sixA"
                     /locus_tag="VN444_01035"
     CDS             complement(1779..2261)
                     /gene="sixA"
                     /locus_tag="VN444_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HWR20425.1"
                     /translation="MVVEKALRHYLVRHGEAKADMEDSARPLSNRGREEVQRVARHAA
                     TLGLEVAQIRHSDKLRARQTAEILAEYLTPRLGIRQIDGLAPGDAPNRVRAELEAAVE
                     PLMLVGHLPHLSRLISALVLGNSEKEIIWPDTGTMVCLAKTERGFRLLWVFTPELAQT
                     "
     gene            complement(2264..2800)
                     /locus_tag="VN444_01040"
     CDS             complement(2264..2800)
                     /locus_tag="VN444_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWR20426.1"
                     /translation="MTPFGVGTRLGIAVGLAALLIGAGGAKMELKSQAFAAGEMIPAK
                     YTCDGQDISPPLRWSDPPAGTIEFALISDDPDAPVGTWVHWVMWNIPVSASALDENLP
                     KTASLLNGTKQGTTDFRRIGYGGPCPPSGTHRYFFKLYALDTALDLPASTTKKDLEKA
                     LQGHILGQAELMGTYRRR"
     gene            complement(2831..4054)
                     /locus_tag="VN444_01045"
     CDS             complement(2831..4054)
                     /locus_tag="VN444_01045"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HWR20427.1"
                     /translation="MVKLTRTERRVRSDYQTALTDWHSTGRPPRGLSSYKLEFGTELL
                     MERPPTAIIESTTIPADHPLFNAHVERPIGEDRPLQRRAFHVAEMATSGSFVPVLSSN
                     DVTIWVCNGDFGEAGKWQPPSRHADCYELYFVHRAHHPLSLITDFGLLKEVREGDFVF
                     LPRGTTYSFLLTGRVSILLYELPKQLFRPYDYWMGDQQPWPFSPAAPIPPEPKPLAAI
                     HPPLSGDETTQVVVKRRLGSFTLLKYAAPVFDVVAWEGEVWPFILRLNDLVALSSPHV
                     HLDPKKLTVFVSEDEGMAMQAFLPRWVHSLPYHHLNWVDEVLFNHKGYGARPEITDGF
                     MTLHPAGLAHGPDLRRLASIARKEASHPKDLPFLEEIAVMVESRSPFVVLKDAEKVEL
                     KGYDRSWYQQSLEIY"
     gene            complement(4100..5503)
                     /locus_tag="VN444_01050"
     CDS             complement(4100..5503)
                     /locus_tag="VN444_01050"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HWR20428.1"
                     /translation="MTHHELRRELIARYGYNLFELKPWDIRVDLLTDSGTTPLSEAQR
                     KAVSRVLRKRRTNERAYAGNESAERLRAALREVFPDFPYVILVHQGRGAERVFCDGFI
                     RSDLWTLSRDPWRQETPHQKRRRPIVIGNAPFDTTRGNLEAAEAEIIDATTRLWHDPN
                     TPLPFKGNVDVELLDHSLMRDGDRMACALITATCNTAAGQPVSTSNFGEVGLRTSSAG
                     TLLVADIARIFLNGFLNKKSIPDDVGITIQEIIKQSLAPVDLCLMSAKKDALSPGGGC
                     ILMKDRALYEALLPYAILSEGGKDYGGMTAETMEAFGVGLKEGLNESFLEEYTGQVAY
                     LGEGLKSAGWPVYEPFGGHAVYLDAGAALDHLAPDDLPGQACAVELYIECGVRACELG
                     TAMAGRDPKTGKNRHPRFEFVRLALPRLRYTRDDLDLVIKGAERLWKRRRDIHGMMFV
                     EPEPPGLRHFTATYKWK"
     gene            complement(5565..>5837)
                     /locus_tag="VN444_01055"
     CDS             complement(5565..>5837)
                     /locus_tag="VN444_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20429.1"
                     /translation="VRYITPILYDDRSRLLQMFTGSLFRLPNPAAGPFIELNQRLDLF
                     DRDPTHFHHATVQALYRCMEEFQEAGTPIWILDFDAIPPDWDPFAE"
BASE COUNT         1212 a         1659 c         1729 g         1237 t
ORIGIN      
        1 ggagatccgg acagggtcgt ctggtagggg agaggacagg gggggcgtcg gcgcctgagc
       61 gagctgttag attgatgatt tcggttatca ccttttcgcc gacattcttt ggtcagacgg
      121 ttgaccgtat agccttggtc agacgtgtgg tgctgttagc gcttatgctc tcagtcgtgc
      181 ccctgctcgc ctcacaggct gcggctcagg acgatgtcgg ccttccaatg catccaaagg
      241 cgattctttc ctccatcgtt cgtcaggccg gagaagggga tgggacgcga tggattcggg
      301 tcagcttcac gaccaatgca ccgtacaagc aagtcgtcaa attctatcag gagaaggtgg
      361 gcggaaatgc gcaaatctct gaggttgact ctgggaagct cctgaacacc cttatcctgt
      421 tcagtaaaga ccccacgaat cagttcagtg tcaatatcaa tagtgcgctt ggaagaaaag
      481 tcactgaggt tgagctatcc cggaactttg ttcatcagta gatagtacgg ggaaggcagg
      541 tagatagact gaaggccgaa gggaagaaaa ggggctatac caaagccttc ggtcttcagc
      601 ctaaacccct gacggctgag cgctgattgc tggattcgat gttgtaagga agtaaaaaag
      661 ttcttgacat cacgatgcat tgcctgtact attcgcttgt cggtgtagca cgcttttgtt
      721 gtggaaggga gagaaccgcg caaagctgct ggatagggct ttcgcggttt ttgtttatcg
      781 gggcatggcc tcgagatttt agatagaggg ggggtgaggt tttggaacga gttactggta
      841 ctgtaaagtg gttcaacgat gcgaagggct acggctttat tgcacgggaa gacggcgacg
      901 atgtattcgt gcactattct gcgattactg gagccggctt ccggtcgctc aatgaagggc
      961 aggctgtgga gttcgaggtt gtggatgggc caaagggtaa gcaggccgcc aacgtctcta
     1021 gagcagccta gttagcttac gttctctctg aaccgttacg acctcccgga gatttctccg
     1081 ggaggtcgtt ttcttttccg agcgctgctt ttctcctgcc ttagcctcga agctcccctt
     1141 cccgctgatg acgaaaacgg cccatgtgac tggcctctcg tcaaccgtcc tcgactcagc
     1201 gctttatggg ggtcaggccg cctggcgagc ggagcgaatc agcagccaga cgcaaaaaat
     1261 cagcttgagc atatcgaggg cggtgtaaat caggtgaaat ctccggaagc gtgccagctc
     1321 aagggacacc agtgtccggg ggacaaagtc caggcttcgt ccgagcgaca ggagtgactg
     1381 gctgagcagc agcgatacga tgacgatgac gaagaccgtt acggcgatag tgatcactgt
     1441 gcggtccagg ggtcttagcc cgagggcgag agctacgatg accgcgccga gcgcaagctg
     1501 ggtgaacccc caggcgctaa agaaaagccg attcaattct gaaacgacgt agcgcaggat
     1561 gccgcgggcc tcatcgtgcg cgaggggggc gatggcgcgc gagaactcaa cggttggcgc
     1621 tgatagcagc cgatcgactg tacgaaaatt ctgcgttgcc acgaacgaca tcagtaaggt
     1681 gccgacgatc cacccaccca gtaacgtgat gagcagcgct ctcatcgttc tcccatcgct
     1741 agcctcagtc ctttcgtttc gacgttcatc ctgatgccct acgtctgagc aagttcaggg
     1801 gtgaagaccc acagcagtcg aaagcccctt tcggtcttgg cgagacagac catcgtcccc
     1861 gtatccggcc agatgatctc cttctcgcta ttgccaagca ccagagccga gatgaggcgg
     1921 ctcaggtgcg gcaggtggcc gacgagcatc agcggctcca cggcagcctc cagttcggcg
     1981 cggaccctgt tcggcgcgtc accgggcgcg agaccgtcga tctgccgaat cccgagccgc
     2041 ggtgtgaggt attcggccag gatttcagcg gtctgccgcg cgcgcagctt atccgagtgg
     2101 cggatctgtg ccacctcaag ccccaaagtc gcggcgtgcc tggccactcg ctgaacctcc
     2161 tcgcgcccgc gattgctcag tgggcgagct gaatcctcca tgtcggcctt cgcctctcca
     2221 tgtcgcacca ggtagtgccg caatgccttt tcgacgacca tccttacctc cggcggtatg
     2281 ttcccatcaa ctccgcttgg ccaaggatat gaccctgcag cgccttctcc agatccttct
     2341 tggtcgtgct tgcggggaga tccaacgccg tatcgagcgc gtacagcttg aagaaatacc
     2401 gatgggtccc ggaaggtgga caggggccac catagccgat ccgccggaaa tcggtagtcc
     2461 cctgtttggt cccgttgagc agcgacgcgg tcttgggaag attctcatcg agcgcactgg
     2521 cgctgacggg gatattccac atgacccagt ggacccacgt gccaactggc gcatcaggat
     2581 cgtccgagat gagggcaaac tctatcgtcc cggccggagg gtctgaccag cgtagtggtg
     2641 gggagatgtc ttgcccatca cacgtgtact tggctggaat catttcacca gcggcaaaag
     2701 cctgactctt cagttccatc ttcgcccctc ctgcgccgat cagcagcgcc gcaagcccaa
     2761 cagcgatacc cagcctggtc cccactccga acggagtcat tgtctcttcc gaagtcttgc
     2821 gacagcgcat tcagtatatc tcaagcgact gctggtacca ggatcgatcg taccccttca
     2881 actctacctt ctccgcatcc ttcaggacaa cgaagggcga cctggattcc accattacgg
     2941 cgatctcctc caggaacggc aggtcctttg ggtgagaagc ctctttccga gcgatgctgg
     3001 ccaagcgcct cagatctggg ccgtgagcca gacctgccgg atgtaacgtc atgaacccgt
     3061 ccgtaatctc gggccgcgcg ccgtacccct tatggttgaa gaggacctca tcgacccaat
     3121 tgagatggtg atacggcagg ctgtggaccc accggggcag gaaagcctgc atcgccatgc
     3181 cttcatcctc gcttacgaag acggtgagct tcttcggatc gagatgaacg tgtggtgagg
     3241 acagcgcaac cagatcgttg aggcgcagga tgaacggcca cacctctccc tcccacgcca
     3301 cgacatcgaa gaccggcgct gcgtatttga gcaacgtgaa actgcccagc cgtcttttga
     3361 cgacaacctg tgtggtctcg tcgccgctga gcggcggatg gatggcggca agaggcttcg
     3421 gctctggcgg aatcggcgca gccggcgaaa agggccaggg ctgctgatcc cccatccagt
     3481 agtcgtacgg gcgaaatagc tgtttcggaa gttcatagag gaggatggac acccttcccg
     3541 tcaagagaaa cgaataggtg gtgcctcgtg gcagaaaaac gaaatcgccc tcccgaactt
     3601 ccttgagcag tccgaaatcg gtaatgaggc tcagggggtg gtgggctcga tggacaaaat
     3661 agagttcata gcagtccgca tgacgagagg gcggttgcca cttcccggct tccccgaaat
     3721 ctccattgca gacccaaatg gtgacatcgt tgcttgagag gacaggaacg aaggagccgg
     3781 acgtggccat ctctgctacg tggaaggctc gcctctggag aggacgatcc tcccctatgg
     3841 gccgttcgac gtgcgcgttg aacaaggggt ggtcagcggg aatcgttgtg ctttcgatga
     3901 tagcggttgg agggcgctcc atcaggagtt ccgtgccgaa ctcgagcttg tagctcgaaa
     3961 ggcccctcgg aggacggccg gtagagtgcc agtccgtaag agctgtctgg taatcagacc
     4021 ggacccgtcg ctcggtccgt gtcagcttca ccatccttgg cacattctcc gtcatgttgt
     4081 cgctccgttc agagagtact cacttccact tatatgttgc ggtaaagtgt ctgaggcctg
     4141 gcggctcagg ttcgacgaac atcattccat ggatgtcgcg gcgtcgtttc cacaatcgct
     4201 ctgcgccctt gatgacgagg tcaagatcgt ctcgtgtata gcgtaggcgt ggaagcgcaa
     4261 gtcgcacaaa ctcgaacctg ggatggcggt tctttcccgt cttgggatcg cgaccggcca
     4321 tcgcggtgcc cagttcgcag gcccggacgc cacactcgat gtagagttca accgcgcacg
     4381 cctgaccagg taagtcgtcc ggcgcgaggt ggtccaacgc agctcccgca tcgaggtaca
     4441 ccgcatgtcc cccgaagggc tcatacaccg gccagccggc agatttcagg ccctcgccga
     4501 gataggcaac ttgtccggtg tactcttcaa ggaacgattc gttgagccct tccttcagcc
     4561 ctacgccaaa cgcttccatg gtctcggcgg tcatgccccc gtaatccttt cccccttcag
     4621 aaagtatcgc gtaaggtagg agagcctcgt agagtgcgcg gtccttcata aggatgcacc
     4681 caccgcctgg actcagcgcg tctttcttgg cggacatgag gcagaggtcg acaggcgcca
     4741 acgattgctt gatgatttct tgtatggtga taccgacatc gtcagggatg ctcttcttat
     4801 tgagaaaacc gttcaggaag atcctggcga tgtcggccac aagaagcgtt ccagccgaag
     4861 aggtccgcaa ccccacctcg ccgaaattgc tcgtgctcac tggctgcccg gccgccgtat
     4921 tgcaggttgc ggtgatcaat gcgcatgcca tgcgatcgcc atcacgcatc aacgagtgat
     4981 cgagcagctc aacatcgaca ttgcccttga aaggcaaggg tgtattcgga tcatgccaga
     5041 ggcgggtcgt cgcgtcaatg atctccgcct ctgccgcctc caggttgccc cgagtcgtat
     5101 caaagggagc gttgccgata acaatgggcc ttcgtcgttt ctggtgcggt gtctcttgtc
     5161 tccaaggatc ccgggacaac gtccagagat ctgatcgaat aaatccatcg cagaacaccc
     5221 gctcggctcc tcgcccctga tggacgagaa tcacatacgg aaaatccggg aagacctctc
     5281 gaagcgccgc tcgcagtcgc tcagcgctct cgttgccggc atacgcccgt tcattcgttc
     5341 tgcgcttcct gagcactcgc gagactgctt ttcgctgcgc ttcagagagc ggcgtggttc
     5401 cagaatcggt caagagatcc acgcgaatgt cccacggctt gagttcaaaa agattgtagc
     5461 cgtatcgggc gattaactct cgacgaagtt catgatgcgt catggcgtgt cgatcctcgc
     5521 ctgtctctct ttgcgacgca gaacgaactg gcaccggtct cgtctcattc tgcgaacggg
     5581 tcccagtccg gcgggatagc gtcgaagtcg aggatccaga tcggtgtgcc ggcctcttgg
     5641 aactcctcca tgcacctata cagcgcctgg accgtggcat ggtggaaatg agtgggatcg
     5701 cgatcaaaca ggtcgaggcg ttgattgagc tcgatgaagg ggccggccgc cgggttcggc
     5761 aagcggaaga gcgagccggt gaacatctgc aggagccggc tgcgatcgtc atacaggatg
     5821 ggggtgatgt agcggac
//
LOCUS       DATMXI010000051         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_173826, whole genome shotgun sequence.
ACCESSION   DATMXI010000051 DATMXI010000000
VERSION     DATMXI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173826"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VN444_01060"
     CDS             complement(<1..534)
                     /locus_tag="VN444_01060"
                     /inference="COORDINATES: protein motif:HMM:NF021210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HWR20430.1"
                     /translation="MAVRSHAQLKQLLDELYHRYSRPVFLSTSALSIPHHYASPKDRE
                     IAAFVTASLAYGNVKQIHRSATTALDAMGRSPARFIRRFDPARDAARFQRFVHRFNSG
                     VDLALLCHLLHQAITAEGSLQAFFLKGYDPTHDDIEPALNSFVERMLSLDVSAFYPSG
                     TLPAKAGVRFFFPSPAQG"
     gene            complement(544..1074)
                     /locus_tag="VN444_01065"
     CDS             complement(544..1074)
                     /locus_tag="VN444_01065"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HWR20431.1"
                     /translation="MELYLSENAFVGLLVSTIEVYRKECFGVLLGQSMSERILVDFVV
                     PYQTANRKFREVHVDLMRSQRVEEAVRSTSRWECVGDYHSHPMYGAVRATTILSAADK
                     HFFKDGNVAIVVAINDSARQQRWNYVQGSGVSGSINGYNIRIAGYYKTNGIIERAPIH
                     CPYAIGFQAKELNSKE"
     gene            complement(1569..2204)
                     /locus_tag="VN444_01070"
     CDS             complement(1569..2204)
                     /locus_tag="VN444_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWR20432.1"
                     /translation="MEWLWDLFHRVYDVETLVRVGGLTALVAIVFVETGLFVGFFLPG
                     DSLLVTAGLFAASGHLELWSLFLFVSLAAIVGDTVGYAIGASTGPKIFTRENSLFFHK
                     KHLTTTKEFYDRYGGITIIIARFMPIVRTFAPLVAGVGGMQYGRFALYNVMGGVGWVV
                     SMTSIGYVLGRTIPDIDRYIHIVIALVIALSLLPGIITFVRSSRRARKPSM"
BASE COUNT          553 a          684 c          722 g          460 t
ORIGIN      
        1 cccttgggct ggtgaaggaa aaaagaagcg aacgccggcc tttgccggga gcgtgccaga
       61 ggggtagaag gcagagacat caagtgacag catccgctca acaaagctgt tcagggcagg
      121 ctcaatatcg tcgtgagtgg ggtcataccc cttaagaaag aaggcctgaa gcgatccctc
      181 cgccgtgatg gcctgatgga gcagatggca gaggagcgcg agatcgacac cggagttgaa
      241 gcggtgaacg aaccgttgga accgggcggc atcgcgggca ggatcgaatc gccggatgaa
      301 cctggctgga ctccttccca tcgcgtcgag cgcagtggtg gcactacggt ggatctgctt
      361 gacgttccca taggcaaggg aggcggtcac gaaggctgcg atctctcggt ccttggggct
      421 cgcgtaatgg tggggaatgc tgagggcgct ggtcgagaga aagacaggtc gactgtagcg
      481 gtgatatagc tcatccagga gctgcttgag ctgcgcgtgg gagcgcacgg ccatggcgac
      541 gactcactcc ttgctgttca gctccttggc ctgaaacccg atggcgtacg ggcagtggat
      601 cggcgctcgc tcgatgatcc cgttggtttt gtagtatccg gcaatccgga tgttgtaccc
      661 gttaatgctg ccggatacgc cgctaccctg aacgtagttc catcgctgct gcctcgctga
      721 atcgttaatg gccaccacaa tggcgacgtt cccatccttg aagaaatgct tatcggccgc
      781 gctcagtatc gtggtcgccc ggacggcgcc atacatcggg tgcgagtgat agtcgcctac
      841 gcactcccag cgcgaggtac tcctgacggc ctcctcgacc cgctgactcc gcatcagatc
      901 gacgtgtacc tcgcgaaact tccggttcgc cgtctggtac ggaaccacga aatcgacgag
      961 gattcgttcg gacatgctct ggccaagcag gaccccgaag cactccttgc ggtagacttc
     1021 aatggtcgag accagtagtc cgacaaaggc gttctcgcta aggtatagct ccatagcctt
     1081 cagtcaccat cggtacgacc taaaaagggt aagctcagct tcgctagatt gaggcgatgt
     1141 gataatgagg cgccgcgact ggtgcgaagc gtgtcgctat cgggttcgcc acggatgatc
     1201 gttcgtgaag atgcgttcgc gtggaggaag aaccggttga agcccctcac tggagattgg
     1261 cggttcgctt acgccgaggc cacttcatca accggaagac ttgccaggca cacagtaagg
     1321 ataacccgcc tcttggaggg aggcaagccc aaaaaaacgg cagccatgcc cggtcaacaa
     1381 ggcggggtta gactggagga acccccgaga agctgcgact attatccgtt tagatacacc
     1441 attgtagggg cgaacctgtg tgttcgcctc atctggacag acatattggt tgtaagagca
     1501 gggcttaccc tgcccaacag cgactaaccc caagcagggc gcagcaagca gcgcccctac
     1561 gggggagatt acatggaagg cttgcgggct cgccggctgc ttcgcacaaa agtaataatc
     1621 cccgggagta aggacagcgc aatgacgagg gcgatgacga tgtgaatgta cctgtcgata
     1681 tccgggattg ttctaccaag gacgtagccg atagatgtca tgcttacaac ccagccgacc
     1741 cctcccatca cgttgtagag agcaaatcga ccgtattgca tgcctcctac gcccgctacg
     1801 aggggggcaa aggtgcggac gatcggcatg aaccgggcga tgatgatcgt aatcccgccg
     1861 taccgatcgt aaaactcttt ggtcgtggtg aggtgcttct tgtggaaaaa cagcgagttt
     1921 tcgcgggtga atattttcgg gccggtgctc gccccgatgg cgtagccgac ggtatccccg
     1981 acaatcgcgg ccaagctgac gaagagaaag aggctccaca actccagatg cccgctggcg
     2041 gcgaacagcc ccgccgtgac aaggagggaa tctcctggga ggaagaagcc gacaaaaagg
     2101 ccggtctcca cgaaaacgat agcgaccagg gccgtgaggc cgccgactcg aaccagggtc
     2161 tctacatcgt acactctatg gaacaaatcc cacagccatt ccatatcagc agcgcctagc
     2221 cgttagccct cagtaagaag caatgatacg cacaacctgc cctacctccg aatacccgaa
     2281 ctcgcctgta accgctctcg ttgttccgca tcggtcccac gctgaaagct gaacgctggt
     2341 ggctgctcaa tcagtaacgg cgccttccga cgctgacgaa accgtgcggg cgtacttggc
     2401 catgacgccg ctcttgtac
//
LOCUS       DATMXI010000052         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_102394, whole genome shotgun sequence.
ACCESSION   DATMXI010000052 DATMXI010000000
VERSION     DATMXI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102394"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VN444_01075"
     CDS             <1..635
                     /locus_tag="VN444_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR20433.1"
                     /translation="ARFERIDLPSPSPFTGVLVDDPLFRIEAVHLDHKIPCLAFALAE
                     VQRINIDPERLKRLGFSAGPWLTEFKRLVRAGASDDTVVRAPCGDETGETFQELLLGD
                     LRDKIVTITNGQRLVYVTDTLYSDENRQKIVALAHDADILFCEAMYLEQDRDRATERH
                     HLTARQAGLLAREANVKELVIFHFSQRYQECQEALYYEAAEAFGGPVRSR"
     gene            752..1354
                     /locus_tag="VN444_01080"
     CDS             752..1354
                     /locus_tag="VN444_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWR20434.1"
                     /translation="MRTIVTAALVLMGVLGPAFPLTWNHVASAEEGTGGPGVMGYRMT
                     PEMMRGFHEMTQGMGIDPSFHRRGGHEGPLISQMLMWQDQLGLTADQERTLRELRANF
                     QKESIKRTAEIDVTELELNGLLEQDKVDMAKVEALAKKSAMLQAELRVGRVKTIEAGK
                     AVLTPEQAEKFDRLGHEWMMGNMGMRMLGPGMPPKRRGAR"
     gene            complement(1405..>2228)
                     /gene="pdxA"
                     /locus_tag="VN444_01085"
     CDS             complement(1405..>2228)
                     /gene="pdxA"
                     /locus_tag="VN444_01085"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWR20435.1"
                     /translation="VTADPGCLEVLDLKNIDIANCPPGVLAPHCGRAAVEYLNKAIDL
                     AIARELDGVVTGPLNKEAMAQAGFKYDGQTELFAERTGTKDYAMLLVVGRMRVLHVST
                     HTSLRTVCDKVKQARVLTVIRLAHQALCDLGSRKKRIGVAGLNPHAGESGRFGREEIE
                     EIAPAVEAAKAEGVRVSGPFPPDTLFHRLKLGEFDAVIAMYHDQGHVPLKLIGFHRGV
                     NVTVGLPIIRTSVDHGTAFDIAGTGTANPRSLVEAILLATKFAHRRRKTARTPDV"
BASE COUNT          454 a          594 c          728 g          452 t
ORIGIN      
        1 gcgctcgctt cgagcgaatc gatctgccgt cgccttcacc gtttaccggc gtactggtcg
       61 acgatccgtt attccggatc gaggcggtac atctcgatca caagatcccg tgtctcgcct
      121 ttgccctggc cgaagtacag cggatcaata tcgaccccga acggctgaag cgcctcggat
      181 tttcggccgg gccctggctg actgaattca agcggctggt ccgcgcgggc gcgtcggacg
      241 atacggtagt cagagcccca tgcggggatg aaaccggcga gacgttccag gagttgttgc
      301 tcggtgacct gagagacaag atcgtgacca tcacgaatgg tcagaggctg gtctatgtca
      361 cagacacgct atattccgat gagaatcggc agaagattgt tgctctggcg catgatgcgg
      421 atattctgtt ttgcgaagcg atgtatctgg agcaggatcg ggatcgtgcg accgagcgac
      481 accacttgac ggcgcggcag gccggactgc ttgcccgcga ggccaacgtg aaggagttag
      541 tgatctttca tttttcccaa cgatatcagg agtgtcagga ggccctctat tatgaggccg
      601 ctgaggcttt cggaggcccg gtccgctcgc gttaaacctt atcccggtat ttggtgtcac
      661 agtacacgga tgtcgcaatc gggcttcggg ttatcccaac ggatatgggt ccatgaaacc
      721 gtagcaacca tttgataagg aggaagtgac tatgcgaaca atcgtgacag ccgcgttggt
      781 tctgatgggt gtgctggggc cggcgtttcc actaacgtgg aaccatgttg cctcggccga
      841 ggagggtacg ggtggaccgg gggtgatggg gtaccggatg accccagaga tgatgcgcgg
      901 gttccatgag atgacgcagg gtatgggtat agacccctcg ttccataggc gggggggcca
      961 tgaagggccg ctcatcagcc agatgctgat gtggcaagac cagttaggac tcaccgcaga
     1021 tcaggagcga accctcagag agcttcgagc caactttcag aaggagtcga tcaagcggac
     1081 tgcggagatc gatgtgacag agctggagtt gaacggccta ctggagcagg acaaggtaga
     1141 tatggccaag gtggaggcgc tggcgaagaa gagtgcgatg ctgcaggctg agcttcgcgt
     1201 gggtcgtgtc aagacgatcg aggccgggaa ggcggtactg acaccagagc aagcggagaa
     1261 gttcgatcga ttgggccacg aatggatgat gggtaacatg gggatgagga tgttgggccc
     1321 aggcatgccc ccgaagcgcc gtggcgcgcg gtgactgacg aatcgatatc gcctgaagat
     1381 agtaggcggg ggtgacgtcc aggactacac gtctggggtg cgggccgtct tgcgccggcg
     1441 atgggcgaac ttcgtggcca gtaggatcgc ctccaccagg ctgcgagggt ttgcggtccc
     1501 tgtccccgcg atgtcaaagg cggtgccatg atccactgag gtgcggataa tggggagacc
     1561 cacggtgaca ttgacccccc ggtggaatcc gatcagcttc agcgggacat ggccctgatc
     1621 atggtacatg gcgatgacgg cgtcgaattc accgagcttg agccgatgga acagggtgtc
     1681 aggggggaaa ggaccgctca ctctgactcc ttcggctttg gcggcctcga ccgcaggcgc
     1741 gatctcttct atctcctctc ggccgaaccg accactctcg ccagcatgag ggttgaggcc
     1801 ggcaacgccg attcgcttct tccgtgatcc gaggtcgcag agagcctgat gagccaggcg
     1861 aatgacggtc aggactctgg cctgtttcac cttgtcgcag acggttcgta acgaggtgtg
     1921 ggtcgagaca tggagaaccc gcatccgccc gacgaccaag agcatcgcgt aatccttggt
     1981 accggttcgc tcggcgaaca gctccgtttg gccatcgtac ttgaaccctg cctgagccat
     2041 cgcctccttg ttaagggggc cggtcacgac tccgtccagt tcacgcgcta tcgcgagatc
     2101 gatcgccttg ttgaggtact ctaccgccgc tctgccacag tggggagcaa ggacgcctgg
     2161 tgggcagttt gcgatatcaa tgttctttaa gtccagcacc tcaagacagc ccgggtctgc
     2221 tgtgacct
//
LOCUS       DATMXI010000053         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_58908, whole genome shotgun sequence.
ACCESSION   DATMXI010000053 DATMXI010000000
VERSION     DATMXI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58908"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /locus_tag="VN444_01090"
     CDS             complement(<1..2006)
                     /locus_tag="VN444_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWR20436.1"
                     /translation="MSHKAGCRIIFNRSIREEYEEMNRPDFTLTRRDLLRLSILTGGA
                     TLIGSRAAWALQDTCGQSSIEDIEQAVTTCGAGLEAMPISPLLLEPFTEDLPIPKALR
                     PGWRRPDGNLDPYTPDAWTCRTSYSGSNNIVYPGPEDGHQDAMGHRPNGAGLPWAGTH
                     QLWPGIDPIYYHIRLQVAEHRVTDKKVRPIGKISKGKGKVIIGPTGADTQLPDSTIYG
                     FNGTFPGPLINAEYGRPVCVRFENDLDKNPDCLDRQDFGAPDWAFLTHLHNGHTAPES
                     DGQPHHLMDNGGGYLPGQWSDNLYLMYPAGNDDREKQSFLWFHDHRMHHTGANVYKGM
                     VGLMPHYDPGVDDGDERHGLRLPGVRINNSDGTFDVKYDIPLALYDCALDDGVTPHAD
                     SHTPLADCGKTHPEWWGKLFFRHYPNHGFVGDIFTVNGKAFPVLRVKRRKYRLRFLGA
                     SIARIYELSLMQGNIGAVPGQQGQYNFGATDRKGNFIRSRGQQCMRLTQIASEGGLLP
                     SPIVRDSFQIWPAKRREFIVDFTKYMDNSPTHDGDVIYLANTMFMPDGRKPEFPGEKA
                     FDANYCVPMLKIIIDGNTPESDDSVMPTTGGLRAMPDRNDPVAQRPTFTLERGGPTEE
                     SQWVINGLEFDPQVALHTVKRGTAEEWTIVNGGGGWVHPMHMHQ"
     gene            complement(2015..>2365)
                     /locus_tag="VN444_01095"
     CDS             complement(2015..>2365)
                     /locus_tag="VN444_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20437.1"
                     /translation="VVEFYDDGGDFPAVGDPARGAAFPTDRRSPLIKLYGDLSVNGLG
                     MAPSEVSDLVAFMLALTDDRVRWERAPFDHPELVIPNGQDGSGNDMTITLPAVGAAGK
                     ATPLRPFLTLDPFQ"
BASE COUNT          432 a          737 c          702 g          494 t
ORIGIN      
        1 tcctggtgca tgtgcatcgg atgaacccag ccacctcctc cgttcacgat ggtccattcc
       61 tcggccgttc cccgcttgac ggtatgcagg gcaacttggg gatcgaactc gaggccgttg
      121 atgacccact ggctctcctc cgtcggaccg ccgcgctcca gcgtgaacgt cgggcgctgc
      181 gcaaccgggt cgttgcgatc cggcatggcg cgcaatccgc cggtagtggg catcacgctg
      241 tcatcagact ccggagtatt accatcgatg atgatcttca gcatcggcac gcaatagttc
      301 gcgtcgaacg ccttctctcc cgggaattcc ggtttgcggc cgtccggcat gaacatggtg
      361 ttcgcgaggt agatcacgtc gccatcatgg gtggggctat tatccatata tttggtaaag
      421 tcgacaataa actcgcgacg cttggccggc cagatctgga aagagtcgcg aacaatcggg
      481 gatggcagca gcccgccctc gctcgcgatc tgggtaagcc gcatgcattg ctgccctcgg
      541 cttcggatga agttgccctt cctgtcggta gcaccgaagt tgtactgccc ctgctggccg
      601 ggaacggcgc cgatattgcc ctgcatgagc gacagctcgt agatccgcgc gatggaggcg
      661 cccaggaaac ggaggcgata tttccgccgc ttgacgcgga ggaccgggaa ggccttgccg
      721 ttgaccgtga agatgtcgcc aacgaaccca tggtttggat agtggcggaa gaacagcttg
      781 ccccaccact cggggtgggt cttaccgcag tcagccaagg gggtatggct atcggcgtgg
      841 ggggtgacac cgtcgtccag ggcacagtcg tacagggcca gcgggatgtc gtacttgacg
      901 tcgaaggtcc cgtcgctgtt attaatccga acgcccggca gacgaagccc gtgcctctcg
      961 tctccgtcgt caaccccggg gtcatagtgc ggcatcaggc ccaccatccc cttgtaaacg
     1021 ttggcccccg tatggtgcat ccgatggtcg tggaaccaga ggaacgactg cttctcccga
     1081 tcatcgttgc cggccggata catcaggtag agattgtcgg accactggcc tggcaggtac
     1141 ccgccgccgt tgtccatcag atgatgcggc tgaccgtcgc tttccggtgc tgtatggcca
     1201 ttgtgcagat gggtcaggaa cgcccagtca ggcgctccga aatcctgacg gtcaagacag
     1261 tcaggattct tatccagatc gttctcgaag cggacgcaaa cgggacggcc atattccgca
     1321 ttgatcagag gcccagggaa ggtcccgttg aagccgtata tggtgctatc cggcagttgc
     1381 gtgtcggccc cggtcggccc gatgatcacc ttcccctttc ctttgctaat cttaccgatc
     1441 ggccgcactt ttttgtcggt cactcgatgc tcagccacct gtagcctgat gtggtaatag
     1501 atagggtcta tccctggcca gagctggtgc gtccccgccc acggaaggcc ggctccgttg
     1561 ggcctgtgtc ccatggcgtc ctggtgacca tcctccggac ctggatacac gatgttgttg
     1621 gagccggaat acgaggtccg acacgtccac gcgtccggtg tgtatggatc caggttgccg
     1681 tcaggcctcc gccacccagg cctcagggcc ttcgggatgg gaaggtcttc cgtgaacggc
     1741 tcgaggagga gcgggctgat cggcatggct tccaggccgg ccccacacgt ggtgaccgcc
     1801 tgttcgatat cctcaatcga cgattgcccg cacgtgtcct gcagcgccca tgcggccctg
     1861 gacccaatga gtgtcgcacc cccggtcaga atcgacaatc tgagcaaatc tcgcctggtc
     1921 aatgtaaagt ccggtctgtt catctcctcg tactcctctc tgatcgaacg attaaaaata
     1981 atacgacagc cagccttgtg actcatgtcc tcgtctattg gaacgggtcc agggttagaa
     2041 atggtctgag cggtgtggcc tttcctgcag cgcctaccgc aggcaacgtt atcgtcatat
     2101 cattcccact cccgtcctgt ccgttcggga tgaccagctc cgggtgatcg aacggcgccc
     2161 tctcccatcg tacgcggtcg tctgtgaggg cgagcataaa tgccaccagg tcgctcacct
     2221 cgctcggcgc catccccagg ccgttcaccg acagatcgcc gtacaacttg atgagcggcg
     2281 agcgacggtc ggtggggaag gcggcgccac gcgccgggtc cccgacggcc ggaaaatccc
     2341 ccccgtcgtc gtagaactcc accac
//
LOCUS       DATMXI010000054         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_277022, whole genome shotgun sequence.
ACCESSION   DATMXI010000054 DATMXI010000000
VERSION     DATMXI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277022"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VN444_01100"
     CDS             complement(<1..1005)
                     /locus_tag="VN444_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWR20438.1"
                     /translation="MATHPMKPERFFLEKFGLTERHLERGLGAALGRQIDDADLYFEY
                     RISESMLLEEGIIKQATKNINQGVGVRALAHEKTGYAFSDEISVENLELAGSRAKGIA
                     ERAGTGSSPPVKVGGTPPHDLYPVRLQPVEIPLEKKIDLLQRIDAIVRKADPRIVQVM
                     ASFACESKIVMIATSAGVMVGDIQPLSRLNITCIAQEGENRQVGSWGGGGRADFEFFL
                     EGGRFERYAKEAARLAIMNLSAADAPAGTMDVVLGPGWPGILLHEAIGHGLEGDFNRK
                     KTSAFSDRIGQQVGSPLVTVVDDGTIPGRRGSLNVDDEGTPTGRTVLIERGILRGYLQ
                     D"
     gene            1319..1897
                     /locus_tag="VN444_01105"
     CDS             1319..1897
                     /locus_tag="VN444_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWR20439.1"
                     /translation="MLQQTQVDRVVPKYKQFLRKYPSIERLASASVSDVEATWRPLGY
                     NVRPVRLHAIAQETVQQHGGKIPASMEKLQAFKGIGRYTAGAVMSFAFRKDTPILDTN
                     VKRLLQRVFLGPMNGNGSKPVKRLWDLSEALIPEGKGYDFNQAMMDLGALICTARKPN
                     CPTCPMRTLCISYPWDKENVTTQVDRLKAEGL"
     gene            2025..2438
                     /locus_tag="VN444_01110"
     CDS             2025..2438
                     /locus_tag="VN444_01110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HWR20440.1"
                     /translation="MSEVARTVEVAAGLIVRDGKILIAQRLSNAPLGGLWEFPGGKRE
                     PGESFEACLMREAMEELGLTIVVDEQVASAEHHDAGRQVRLRFYRCTVLAGEPSPLGC
                     EAFCWVTPAEIAAYSFPPADLPLVYQIASGQRIIA"
BASE COUNT          498 a          768 c          745 g          531 t
ORIGIN      
        1 atcttgcaga tacccccgca ggatcccgcg ctcgatcagg acggtgcgtc cggtcggggt
       61 tccctcgtca tcgacattca gcgagccacg gcgtcctggg atcgtgccat cgtccaccac
      121 ggtgacaagt ggagagccca cctgctgccc gatccgatcg gagaaggccg aggtcttctt
      181 ccggttgaag tcgccttcaa ggccgtggcc gatcgcctcg tgcagcaaga tgccgggcca
      241 tccggggccg aggacgacgt ccatcgttcc ggcaggggcg tcggctgcac tgaggttcat
      301 gatggccagg cgggcggcct ccttggcgta gcgctcgaag cggcctccct ccaggaagaa
      361 ctcaaagtcg gcccggccgc ctccgcccca gctccccact tgtctgttct cgccctcttg
      421 ggcgatgcag gtgatgttca ggcgtgagag cggctggatg tcgccgacca tgacgccggc
      481 agaggtcgcg atcataacga tcttagactc gcaggcaaat gatgccatca cctggacgat
      541 ccggggatcg gctttcctga caatggcgtc aattcgctgg agcagatcga ttttcttctc
      601 cagcggaatc tctaccggct ggagacgaac cgggtacaga tcatggggag gcgtgccgcc
      661 aaccttcacc ggcggagagc tcccggtgcc cgcccgttca gcgatcccct tggccctgga
      721 gccggcgagt tccaggttct ccacactgat ctcgtcggaa aaggcgtacc cggtcttctc
      781 gtgagcgagg gcccggacgc cgaccccctg gttgatattc tttgttgcct gcttgattat
      841 cccctcctcg agcagcatgg actcgctgat ccggtactcg aagtagagat cagcatcatc
      901 gatctgtcgg ccaagggcgg cccccaatcc ccgttcaagg tgccgctccg tcagcccgaa
      961 cttttccagg aaaaaccgtt ccggtttcat cgggtgtgtc gccatctatg tcctcactcc
     1021 cttttagtcg ttcagcaatc agctattagc gatgtagggg cgctgcttgc tgcgccctct
     1081 ctgggcagga caagccctgc ccctacgctg atagctggcg gtgaatgcct gaatggtatt
     1141 gaaatcgacc ttacaacttc ttatactacg gcccatgccg cccgtcaaca atcagttccc
     1201 ggatccagcc atcaggcgaa aatttcagca gagactcttg cgctggtatg cgcgcaatcg
     1261 gcgagaccta ccttggcgaa agacctctga cccgtataag atcctggtct ccgaggtgat
     1321 gctgcaacag actcaggttg atcgggtcgt ccccaagtat aagcagtttc tccggaagta
     1381 cccctcgatc gagcggctcg ccagcgcctc tgtgagcgac gtcgaggcca cctggcggcc
     1441 gcttggctac aatgtccggc cggttcgcct gcacgccatc gcacaggaga ctgttcagca
     1501 acatggcggc aagatcccgg cctccatgga gaagcttcag gcgttcaagg ggattggccg
     1561 ctataccgcc ggtgcggtga tgagcttcgc ctttcgcaag gacaccccga tcctcgatac
     1621 caacgtgaaa aggctgctac agcgggtctt ccttggtccg atgaatggga acggctccaa
     1681 gccagtgaaa cgtctctggg acctctcgga agccttgatc ccagaaggca aagggtatga
     1741 cttcaatcag gccatgatgg acctcggcgc gctcatctgc accgcccgga agcccaactg
     1801 cccgacctgc ccaatgcgga cactctgcat ctcttaccca tgggataagg aaaacgtaac
     1861 gactcaggta gataggctga aggctgaagg cttgtagggg tacgtcatgc gtgatcatac
     1921 acaactccga aaaggttttg aatggcttga ttcgtgcctt gcgagataat ttagaatctt
     1981 cagccttcag cctaaacacc tgagtagtta caggaaagca agtcatgtct gaagttgcgc
     2041 ggacagtcga ggtagcggcc ggcctcatcg tcagggacgg gaagatcctc atcgcccagc
     2101 gcttgagcaa tgctcccttg ggaggactat gggagtttcc cggcggcaag cgagagcctg
     2161 gtgagagctt cgaggcttgc ctgatgagag aggccatgga agaactgggg ttgacgatcg
     2221 ttgttgacga acaggtcgcc tccgccgagc accacgatgc cgggcgccag gtccgactcc
     2281 gtttctatcg gtgcaccgtt ctggccggtg aacctagccc cctcgggtgt gaagcctttt
     2341 gctgggttac tccggcggaa atcgccgcct attcgtttcc gccggcggac ctgcccttgg
     2401 tctaccagat cgcctctggc cagcgtatca tcgcttagtg attccaatag cgactgaact
     2461 cgggaaggta ggacagggtc ttgaggctgg gcatccgatc gataaagagc ttgcccagca
     2521 ggtggtcgcg ctcgtgttgg ag
//
LOCUS       DATMXI010000055         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_151752, whole genome shotgun sequence.
ACCESSION   DATMXI010000055 DATMXI010000000
VERSION     DATMXI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151752"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..695
                     /locus_tag="VN444_01115"
     CDS             201..695
                     /locus_tag="VN444_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWR20441.1"
                     /translation="MRRAIAADPAPPEYQVKAAFLYQLIRFIEWPPQAFRTGHDKVII
                     GVLGEGPMNGALSAVQGKTAKGRTVAIERYKTVADLDYCHVLFISSSMDERLPAILKR
                     MKGWNALTVGDVEGSARRGAMINFITVEDTIRFEVNVAAAKRANLRISSELLELAHAV
                     HGVP"
     gene            697..>2098
                     /locus_tag="VN444_01120"
     CDS             697..>2098
                     /locus_tag="VN444_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR20442.1"
                     /translation="MRSLRDVPIRQKLARITRLAMAVALGMVAVAFLVLELLTFRSIM
                     TKEMRSMAAITGTNSRAALAFVDPAAGKRTLAAFGADPRIMTARLYTKEGRVFATYHR
                     GDVKPDMIPAIPPSNGVSLRARSLTLVQPIHLDGERIGTIYLQADMAALYDRLLQYAL
                     TMIVVLGFASLVALAHSSRLQGMVSGPVTHLAQMVKAVTEQDDYSIRAIKSGQDELGL
                     LTDGFNEMLARIQERDAALQKAREELEERVNLRTQELQLEIAERRRTEEALRKLSRAV
                     EQATDIVLITNREGVIEYVNPAFERHTGYSRDEAIGRTPRLVKSGQQSQEYYERLWQK
                     LLAGEVFHGDFVNRKKDGTLYFEEEIITPLKDSQGAITHFVSAGRDITERKRAEADLV
                     RAHAELQRQTKALRLEVKERTRAEAEARSSQEQLRALTAHLENVREEERTWISREIHD
                     ELGQLLTGIKFDLSWVS"
BASE COUNT          431 a          614 c          675 g          378 t
ORIGIN      
        1 tcgtcattgc gagcgaccga agggagcgtg gcaatctcac cgtcgttgtc ttgaaggact
       61 gtgagattgc ttcggcttcg cctcgcaatg acacggggga cttctaacca atgacgggcg
      121 cactcatgcg atccaatctg agatacttgc tcagtaagac cgtgatctgt atgatgttag
      181 taagctgcgc tggagggcag gtgcgccgtg cgatcgccgc cgatccggcg ccgccggagt
      241 accaggtcaa ggccgcgttt ctgtatcagc tcatcaggtt catcgagtgg ccccctcagg
      301 ccttccggac cggccatgac aaggtgatca tcggcgtgct tggcgaaggc cccatgaacg
      361 gcgcgctgag tgcggtccag ggcaagacag caaagggtcg gacggtggcg attgaacggt
      421 acaagacggt tgcagacctg gactactgtc atgttctgtt tatcagttcg tccatggatg
      481 aacggctccc ggccatcctg aagcggatga agggatggaa cgcgctcacg gttggtgacg
      541 tggaggggtc ggccaggcgc ggcgcgatga tcaacttcat caccgtcgaa gatacgatcc
      601 gttttgaggt caacgtggcg gccgccaagc gcgcgaacct gaggatcagt tcggaactgc
      661 ttgagctggc tcacgctgtc cacggggtgc cgtaacatgc gttcgcttcg ggatgtcccg
      721 atccgacaga agctggcgcg tattacccgg ctcgccatgg ctgtggcgtt agggatggtc
      781 gccgtggcct tcctggtgct cgaattgctg acgttccggt cgatcatgac gaaggagatg
      841 cgcagcatgg ctgccatcac cgggacaaac agcagggcgg cgctggcctt cgtggatccc
      901 gccgccggca agcggaccct ggcggccttt ggcgccgacc cgcggattat gacagcgcgc
      961 ctctacacga aggaggggcg ggtcttcgcc acgtatcatc gcggagacgt taagccggac
     1021 atgattcccg ccatcccccc atccaacggc gtatccctcc gggcgaggag cctgaccctt
     1081 gtccagccca ttcacctgga tggcgagcgc atcggcacga tctacctcca ggccgacatg
     1141 gccgcgctat acgatcgact cctgcagtat gcgctcacca tgattgtggt gctgggattc
     1201 gcctccctgg tggccctggc gcactcatcc cgactccagg gcatggtctc cggaccggtc
     1261 acacatctcg cgcagatggt caaggccgtg acggagcagg atgactactc gatccgtgcg
     1321 atcaagtccg gccaggacga actgggcctg ctcaccgacg gtttcaacga gatgctggcc
     1381 cgaatccagg aacgggatgc ggcgctccag aaggcgcgcg aggagctcga agaacgggtc
     1441 aacctccgca cacaagagtt gcagcttgag atcgccgagc gaaggcggac cgaagaggcg
     1501 ctcagaaaac tctcccgcgc cgtcgaacag gcaaccgaca ttgtgctgat caccaatcga
     1561 gaaggcgtca tcgagtatgt caaccccgcc tttgagcgac acaccggcta ctcacgggat
     1621 gaggcgatcg gccggacgcc acggctcgta aagtcaggcc aacagtcgca agagtactat
     1681 gagcggcttt ggcagaaact gctcgccgga gaggtctttc atggcgactt cgtcaaccga
     1741 aagaaggatg ggaccttgta ttttgaagaa gaaatcatta cgccccttaa ggacagccag
     1801 ggcgctatca cccattttgt gtcggccggg cgcgacatca ccgagcgcaa gcgggcggag
     1861 gcggacctgg tgcgtgccca tgccgaattg cagcgccaga ccaaagcgct gcggctggag
     1921 gtgaaagagc ggacgagggc ggaggcggag gcgcgcagct cacaggagca gcttcgcgcc
     1981 ctgacggctc atctggaaaa tgtgcgggag gaggaacgga cgtggatctc ccgggagatc
     2041 cacgatgagc tgggacaact gctgaccggc atcaagttcg acctctcatg ggtgagcc
//
LOCUS       DATMXI010000056         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_151712, whole genome shotgun sequence.
ACCESSION   DATMXI010000056 DATMXI010000000
VERSION     DATMXI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151712"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /gene="rhuM"
                     /locus_tag="VN444_01125"
     CDS             <1..460
                     /gene="rhuM"
                     /locus_tag="VN444_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HWR20443.1"
                     /translation="IHGHTAAEVIVDRADAGKQHMGLTTWKDAPHGKIQKFDVSVAKN
                     YLTEPEMAQLSRLVNAYLDVAEDMAQRQIPMTMQDWETRLNRFIAATDREILQDAGKV
                     TAEIAKAHAESEFEKYRIVQDRLFVSDFDAEVMRISRNEDKSGNDEGGAE"
     gene            441..1754
                     /locus_tag="VN444_01130"
     CDS             441..1754
                     /locus_tag="VN444_01130"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWR20444.1"
                     /translation="MKEVRSERRTEPTDRGMSDWQKTTVGEIASGFLSGGTPSTSRAD
                     FWEGEIPWITSKWLGDKLELITGEKFVSEEAVKKTATKIVPKGSIIFATRVGVGKVGI
                     NRIDLAINQDLAGVLIDNERYDIKFLAYQLGIDSIQQYVAMNKRGATIKGITRDCLEQ
                     IRLNLPPLPEQKKIARILSTVQRAIESQERIIQTTTELKKALMHKLFTEGLRKEPQKQ
                     TEIGPVPESWEVVGLGEVAKIERGKFSHRPRNEPRFYGGEYPFVQTGDVSNCDGYVRN
                     YTQTLNEDGLAISKMFPAGTILITIAANIGFTGILKFNSACPDSLIGITPNENLHTEF
                     LNYYLITQQSEMDRLAPKGTQKNINIQFLKPWPVPLPSFEEQTDIAEAFSTTDRKIRL
                     ATQKRNEFQDLFRTLLHELMTAKTRVANINLANATCNYFEDLSTP"
     gene            1814..>2136
                     /locus_tag="VN444_01135"
     CDS             1814..>2136
                     /locus_tag="VN444_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HWR20445.1"
                     /translation="MSAEDALEGTLYIGYPIIGTPEGSFPIDALLVSPQKGLILFNLI
                     EGRDFGEYGDAQDEAFNKMQAKLLQHQSLIHKRKLRANIHTVTFAPVVANLNAHTKDD
                     YPLCNS"
BASE COUNT          578 a          620 c          546 g          392 t
ORIGIN      
        1 gatccacggt cacactgccg ccgaggtcat cgtggaccgt gccgatgccg ggaagcaaca
       61 catgggcctg accacatgga aagatgcgcc ccacggaaaa atccagaaat tcgatgtcag
      121 cgtcgccaaa aactacctga ccgaaccgga gatggcgcaa ctctcccgcc tcgtcaacgc
      181 ctacctcgat gtcgccgaag acatggccca gcggcagatc cccatgacca tgcaggactg
      241 ggaaacacgg ctcaaccgct tcatcgcagc caccgaccgg gaaatcctgc aggacgccgg
      301 caaagtcacc gccgaaatcg ccaaagccca cgccgaatcc gaattcgaaa aataccgcat
      361 cgtccaggat cgcttgtttg tctcggactt cgacgccgaa gtcatgcgga tctcaaggaa
      421 tgaggataaa agtggaaacg atgaaggagg tgcggagtga gcgccgaacg gagccaacgg
      481 atcggggtat gagcgactgg cagaaaacta cagttggaga gatcgcctcc ggattcctca
      541 gcggtggaac gccatcgaca tcgcgggccg atttctggga aggagagatt ccttggatca
      601 cgagtaaatg gctcggagac aaacttgagc tcattaccgg agagaaattc gtctccgaag
      661 aagcagtcaa aaagactgca accaagatcg tccccaaggg cagcatcatc ttcgcaacgc
      721 gggttggcgt tggcaaagtc ggcatcaacc ggattgacct cgccatcaac caggacctcg
      781 caggcgtcct gatcgacaac gagcgctacg acatcaagtt tctcgcatac caattgggga
      841 tcgactcgat tcagcaatac gtcgcgatga acaagcgcgg cgcgacgatc aaagggatca
      901 cgcgagattg tctcgaacag attcggctca acctcccccc actccccgag cagaagaaga
      961 tcgcgcgcat cctttcgacg gtgcagcggg cgatcgaatc gcaggagcgg atcattcaga
     1021 ccaccaccga gctgaagaag gccctcatgc acaagctctt caccgaaggc ctccgcaaag
     1081 aaccccaaaa acaaaccgaa atcggccccg tccccgaaag ctgggaggtg gtggggttgg
     1141 gtgaagtcgc caagattgag cgtggcaaat tttcccatcg tccacgcaac gagccccgtt
     1201 tctacggcgg cgagtatccc ttcgtccaaa ccggagacgt ttccaattgc gacggctatg
     1261 tccgcaacta cacgcagacg ctcaacgagg acgggcttgc catcagcaag atgtttcccg
     1321 caggcaccat tctcatcacg attgctgcca acatcggatt tacagggatt ctgaagttta
     1381 attccgcatg cccggacagc ctcatcggga taacacccaa tgagaacctt cacactgagt
     1441 ttctgaacta ctatctgatt acgcagcagt cggagatgga ccgactcgct ccgaagggaa
     1501 cgcaaaagaa cataaacatc cagttcctca aaccctggcc ggtccctctg ccatcgtttg
     1561 aagagcaaac cgatatcgcg gaagcattct cgacgaccga tcggaagatc aggctagcga
     1621 cccaaaagcg taacgaattc caagacctct tccgtaccct ccttcacgaa ttgatgaccg
     1681 caaagacccg agtcgccaat ataaatttgg ctaacgcaac ctgtaactat tttgaagacc
     1741 tatccacacc atgattaaca cagtttgggg tagcactgta aagccgatat ccagcaagca
     1801 actggtgctg actttgtcag cagaagatgc tctggagggc acgctctata tcgggtatcc
     1861 gatcatcggg acgccggaag gctcgtttcc gattgatgca ctattggtat cgccgcagaa
     1921 aggcttgatc ctcttcaatc tcatcgaggg gcgggatttc ggtgaatatg gagatgcaca
     1981 ggacgaggcg tttaacaaaa tgcaagccaa gctcctgcag caccagtcgc tgatccataa
     2041 acgaaaactc cgagcgaaca ttcacactgt gacgtttgcg ccagtggtgg cgaacctcaa
     2101 cgctcacacc aaagacgatt acccgctctg taattc
//
LOCUS       DATMXI010000057         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_263053, whole genome shotgun sequence.
ACCESSION   DATMXI010000057 DATMXI010000000
VERSION     DATMXI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263053"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VN444_01140"
     CDS             <1..810
                     /locus_tag="VN444_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR20446.1"
                     /translation="IQKMVDEFQRLYGPVQEFVKSAEQMDMNLPLEFHVRIEESEFQD
                     QFFNRLNRQVRGTFSGVEESNQLLRRLLQEASFAAADDAVAFAETIDSALRADKRDGQ
                     QERETRLAEQLRKGVEPIEVLDYLFGFDYLSPRYSLTYDGQEIGQLSPGERGLLLLVF
                     YLLVDKDDIPIVIDQPEENLDNQTIYRVLVKCIKKAKERRQVIMVTHNPNLAVVCDAE
                     QIIYASCDKAASRFDYESGAIEQPEIKDRVVQILEGTEPAFKNRQSKYRLG"
     gene            881..1132
                     /locus_tag="VN444_01145"
     CDS             881..1132
                     /locus_tag="VN444_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20447.1"
                     /translation="MSYGGRSFFASVGDWIGGLGVAFRAALSTPLESGRADSDEKTVD
                     PDFDGWQHGDPMVTRDPPSGNCQGTCRLEGLTHGLAHAA"
     gene            complement(1419..>2969)
                     /locus_tag="VN444_01150"
     CDS             complement(1419..>2969)
                     /locus_tag="VN444_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20448.1"
                     /translation="WMQKAASANHRPAQAKLGEAYATGSGLEQDFRKALHWFRAAGFT
                     QPRACLGMGRLYEQGLGVAKDSGQANMWFVCAASFGTAPDLQGKTPEIARLTLAEIAS
                     AYAQLAENFAKGTVDFPQNDVTAYVCYALATQIADNGQYGAALHAAKKKMLSFEPNQV
                     HDWNRMIQEQLNPLIERWRLNILSGWEPTVAVNLLRAFARLGDRDSQSFLATIAGVGR
                     DGLPLDPVSAYAWYSIAQNRHDAQPTAVHTNRAGLVKAIQGNPPSSETARPEELLNKH
                     LSKEQLAEAQLMVGEFFQSGLRVPLGNKFQDYGQAAKWYAMAADNGNAEAQSRLGSAY
                     APDAGAANDPALTQLFNLWSRSDFDIVQEQEHWNKIRANLRKEIEDEWLQKHASEFQQ
                     AHESDQLGLNLVNELLDYDAYRVSENAAQVFTRLAIDILRWWAERNNAEAQYRLGTAK
                     SSGILGKSRIQVDHNEAAQWLLKAAEAGHKEAQFRLANAYEKGLGVTKDPSEARKWSL
                     KSGISHLD"
BASE COUNT          572 a          841 c          895 g          661 t
ORIGIN      
        1 atccagaaga tggtcgacga gttccagcgg ctctacggcc cggtacagga gttcgtgaag
       61 tcggctgagc agatggacat gaacctgccc ttggaattcc acgtacggat tgaggaaagc
      121 gagtttcagg atcagttctt caatcggctc aaccggcagg ttcggggcac tttctccggt
      181 gttgaggaga gcaaccaact cctacggcgc ctgctgcagg aagccagctt tgccgccgcg
      241 gatgacgccg tggcgtttgc cgagacgatc gatagtgcgt tgcgcgcgga caaacgcgat
      301 gggcagcaag aacgagagac tcgcttggct gagcagcttc gcaagggcgt tgagccgatc
      361 gaggtgctcg actacctgtt cggcttcgac tacctctctc cgcgttactc gctgacctac
      421 gacggtcagg agatcggtca gctctccccc ggagagcgag ggctgctact gttggtcttc
      481 tacctgctgg tggacaagga tgacatcccc atcgttattg accagccgga ggagaacctt
      541 gacaaccaga cgatctacag ggtcctggtc aagtgcatca agaaggcgaa ggaacgacgt
      601 caggtgatca tggtcacgca caaccccaac ctggcagtcg tatgtgatgc ggagcagatc
      661 atctacgcat cgtgtgacaa ggctgcctct cgctttgact acgagtctgg cgcgatcgaa
      721 cagcccgaga tcaaggaccg tgtcgttcag attctcgaag gcacagaacc ggcgttcaag
      781 aatcgtcaat cgaagtaccg gctgggatga gcggcagcgg caatgaccga agggcgtgca
      841 gcgtatcctc gactcggtgt gcagggagcg ttgtagccat atgagttacg gcggcaggtc
      901 gtttttcgca tccgtaggcg actggatcgg tggcttgggc gtcgcatttc gtgccgcgct
      961 gtcgactcct ttggagtctg gccgtgcgga ctccgacgaa aagacggtcg atcccgactt
     1021 tgacggctgg cagcacggtg acccaatggt cacccgggat cctccgagcg gtaattgtca
     1081 aggtacctgt cgcctcgaag ggttaaccca cggcttggct catgcggctt gagcctgggt
     1141 gtgggcggtt gtccggtcgc gtggtttctc gggtttgaga aaggcgaccg aaatcggttg
     1201 ccagttgcgg ctgtgtcggc tccagcgctc ggggtgtctg gcccgcgcgg cttcgtaggc
     1261 cgtgctcacg atgcgtcgat cgttcagctg acagtgccat gatgatcgag cagtgcgaca
     1321 gagcggcaca ggcccaattc cccttggatc tggcagcggt tctccaagca gaacgcactt
     1381 accctgcccg tcggcagttc atccgagaag ttcctgtact agtccaaatg agaaatgccg
     1441 gatttgaggc tccacttcct ggcctcggac ggatccttgg ttacaccgag gcccttttca
     1501 taggcgttag cgaggcgaaa ctgggcttcc ttatggccgg cttcagccgc cttgagcaac
     1561 cactgggctg cttcattgtg gtcaacttgg atcctggact ttcccaaaat tccggatgat
     1621 tttgcggtcc cgagtcgata ctgggcctcg gcattattgc gctcggccca ccagcgcaaa
     1681 atatcgatgg ccaggcgagt gaagacttgg gccgcgtttt ccgaaactcg ataggcgtca
     1741 tagtccagga gttcgttgac cagattcaat ccgagctggt cggattcgtg cgcctgctgg
     1801 aactcgctgg catgcttctg gagccactca tcctcaattt cctttcgcag gttcgcacgt
     1861 atcttgttcc agtgttcctg ctcctgaacg atatcgaaat cggatcgaga ccagagattg
     1921 aataactggg tcaaggccgg atcgttcgcc gcgccggcgt ctggtgcgta cgcggagcca
     1981 agtcgggatt gggcttcggc atttccgtta tcggcggcca tcgcgtacca ctttgccgcc
     2041 tgtccgtaat cctgaaattt gtttcccaag ggcactcgaa gccctgactg gaaaaactct
     2101 ccgaccatga gctgagcttc tgcgagttgt tccttgctca ggtgcttgtt gagaagctcc
     2161 tccggtctcg ctgtctcgga ggaaggcgga ttcccttgaa tggccttcac caaaccggcc
     2221 cggttcgtgt gaactgcggt cggctgagca tcgtggcgat tctgggcaat ggaataccag
     2281 gcatatgccg acacgggatc cagtggcagg ccatcacggc ccacgcccgc tattgtcgcc
     2341 aggaaactct gggaatcccg gtcacccaaa cgggcgaacg cgcgcaatag attgaccgcc
     2401 acggtcggtt cccatcccga caggatgttc aagcgccatc gctcgatcaa tgggttgagt
     2461 tgttcctgga tcatgcggtt ccagtcgtgg acctggtttg gctcgaagct gagcattttt
     2521 ttcttcgccg catggagcgc cgcaccgtat tgtccgttgt cggcgatctg agtcgccagc
     2581 gcataacaaa cgtaagcagt gacgtcattt tgcggaaaat ccaccgtgcc cttcgcgaag
     2641 ttttccgcca gctgggcata ggccgaggcg atttccgcga gggtcagtct cgcgatttca
     2701 ggggttttgc cttgaaggtc cggagcggtt ccgaaagatg ccgcgcacac aaaccacata
     2761 tttgcctgcc cggaatcctt tgcaaccccc aggccctgct catagaggcg gcccatgcct
     2821 aggcacgccc tgggttgggt gaagccggcg gcgcggaacc aatgaagtgc cttcctgaaa
     2881 tcctgttcca gacccgagcc ggtggcgtag gcctctccca atttcgcttg ggcggggcga
     2941 tggttggcgg aagctgcttt ctgcatcca
//
LOCUS       DATMXI010000058         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_151116, whole genome shotgun sequence.
ACCESSION   DATMXI010000058 DATMXI010000000
VERSION     DATMXI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151116"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VN444_01155"
     CDS             <1..769
                     /locus_tag="VN444_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWR20449.1"
                     /translation="EILRTSLLPSLMECLASNLRQDEAGPRLFEIGRVYLPRHADVHE
                     ERQMLVIGMAGPRWQRQWSRAAAQLDFYDLKGVVEELLDRLNLGETRFIQIMDQYPFH
                     PGRTTAVYSKDARLGVMGELHPAVARNFEVRVPVCLAEFDLEQLLSVIGGEFLRIVPP
                     PRFPAVRRDLALIVPERVPVSELFEAIRQAGRPLLYHVELFDLFRGEGLAPGQKSCGV
                     GLIFRSSDHTLTDAEVAQVEACIIQQLQRRTGARLRG"
     gene            841..1158
                     /locus_tag="VN444_01160"
     CDS             841..1158
                     /locus_tag="VN444_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20450.1"
                     /translation="MVEHPSDQGRMMLAEKLEDLEGRVRTMVALATKLKEEKAGLGQR
                     MEELQAVIKAQVEQVGALEAARRKDQEQLIRMQEEREEVRLKVDHLLEEIERIEASVE
                     PGD"
     gene            1166..1501
                     /locus_tag="VN444_01165"
     CDS             1166..1501
                     /locus_tag="VN444_01165"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWR20451.1"
                     /translation="MEQLVSVVIRGEQYTIRATEDPSYIRRVAAYVDAKLDGVAKGSQ
                     SLSSTKAIVLASLHIADELFKAEAERERSERLVAMKLGTLSGLLGTALEDGASGPMGG
                     DQESEASGA"
     gene            1553..1738
                     /gene="ssrS"
                     /locus_tag="VN444_01170"
     ncRNA           1553..1738
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VN444_01170"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1912..3477
                     /gene="rny"
                     /locus_tag="VN444_01175"
     CDS             1912..3477
                     /gene="rny"
                     /locus_tag="VN444_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWR20452.1"
                     /translation="MAIGIDQLVIALIVAIVGVVVGYLLRKQVVESKVAEAETLASRI
                     LQEAKKEAETKHREVELEAKELALQAKADLERETKSRRQELEGVQRNLGQKEETLDRR
                     QEQLERRDRELDSLVTALGNREQAAASKETQCAQLMEDAQRQLERIAGLTAEEGKKIL
                     LQNLEQEARIESMALFKQIEDETRATAGEKAKEILAIAVDKCAQEFVMESTVSVVDLP
                     SEEMKGRIIGREGRNIRAFERATGIDVIVDDTPEAVILSGFDPIRRAVAKEALQRLIT
                     DGRIHPARIEEVVEKVKGEIDDDIKKTGERTAFELGISGIHPELMRLVGRLKYRTSYS
                     QNQLIHTNEVTQLAGVIAAELGLDVKVAKRAGLLHDIGKSADVNMEGTHVSISAELAK
                     KYGERPKVVNAIAAHHEDVEITCLEAVVLQIADTLSAARPGARRELLESYVKRLEGLE
                     RIANSFAGVSKTYAIQAGREVRIIVESDKVSDVQAYQLAREIAKRIEEEMQYPGHIKV
                     TVIRETRAVEYAK"
BASE COUNT          818 a          885 c         1183 g          718 t
ORIGIN      
        1 ggagatactt cggacatcgc tgctcccaag cctcatggag tgtctcgcga gtaaccttcg
       61 ccaggatgag gctggtcctc gtctgttcga gatcggtcgg gtctatctac cgcgccacgc
      121 cgacgttcat gaggaacggc agatgctggt catcggtatg gcggggccaa gatggcagag
      181 gcaatggagc cgggcggctg ctcaattgga tttttatgat ctgaagggcg ttgtggaaga
      241 gttgcttgat cgcctgaatc tcggagagac gcgcttcatt cagatcatgg atcagtatcc
      301 gtttcatccc gggcgaacga cggccgtgta ctcgaaggat gcgcggcttg gggtgatggg
      361 agagctccac ccggcggtgg cccgcaactt tgaggttcgg gtcccagtct gcctggctga
      421 gttcgatctg gaacagctcc tgagtgtgat cggtggtgag ttcttacgga tcgtcccgcc
      481 gcctcgcttc cccgcagtgc gaagggacct ggcgctcatc gttccagagc gcgtcccggt
      541 ctcggagctg tttgaggcta tccggcaagc cgggagaccg ctgctctacc atgttgagct
      601 cttcgatctg ttccgtgggg aagggctggc gccagggcag aaatcgtgcg gcgtcgggct
      661 gatcttccgt tcctcggatc acaccctcac cgatgccgaa gtggcccagg tcgaggcctg
      721 cattattcag cagttacagc gacggaccgg agcgcggctg agaggataag tttcgcttta
      781 caagggtaca ttactttggt aggctctcga ttggataagg actgcaaccg cggagacctc
      841 atggtagagc acccttcaga ccaaggccgg atgatgcttg ccgaaaagct ggaagatctt
      901 gaaggccgag tgaggacgat ggttgcgctt gcgacgaagc tgaaggaaga gaaggcaggc
      961 ctggggcagc ggatggagga actgcaggct gtaatcaagg cgcaggtgga gcaggtgggt
     1021 gcgctcgagg ccgcacgaag aaaggatcag gaacagctca ttcgaatgca ggaggaacgt
     1081 gaggaggtcc ggctcaaggt agatcacttg ctggaggaga ttgagagaat cgaggcctcc
     1141 gttgagcctg gcgattgagg gttgcatgga acagcttgtc agcgtcgtga tccgaggaga
     1201 gcagtacacg atccgtgcta cagaggatcc atcgtacatc cgaagggtcg cggcgtatgt
     1261 cgatgcgaaa ctggatggag ttgcgaaggg atctcagtcg ctctcatcca ccaaggcgat
     1321 cgtcttggcc tcattgcata ttgcagatga gctttttaaa gcagaagcag agagggaacg
     1381 atccgagaga ttggtagcga tgaagctggg tacactatca ggactgttag gaaccgccct
     1441 tgaagatgga gcaagcggtc ccatgggcgg tgatcaggag tccgaagcga gtggagcata
     1501 ggtggagaaa gagggctgcg gtgcagtata atgcgccgca tgaagtcagc gagaccccct
     1561 gccgtgttcg tgatgagggc acgtgttttg aaccaacagt gaaaaagagg ggccctgttc
     1621 gtagcgtggt gtgcatagcc ccaccttatg tggggagagc ctgaagcgct gcctgggcaa
     1681 ccacctgatt attcaggttc aaacctcatc cctcaaacgg cacaaaggtg gggggttctt
     1741 ctcttttcac ctctggtgtc accgctcagt tccagatccg gctacccttt gcatccttcc
     1801 tgcctcggtc cgaaggccct cttcgtattc ccctcttggt gatcctctcg tatttctcgc
     1861 catgttttca atggcgttgg cataggtact tcggaaggag gtggacctcc cttggcgatt
     1921 ggaattgatc agctagtgat cgcgttgatc gtggcgatcg taggtgtagt agttgggtat
     1981 ctgctcagaa aacaggtagt cgaaagcaaa gtggccgagg cagagaccct ggcctcgcgt
     2041 atcctgcagg aggcgaagaa agaggcagag accaaacatc gggaggttga gcttgaggcc
     2101 aaggagctcg cccttcaggc aaaggcagat ctggagcgtg agaccaagag tcgtcgccag
     2161 gagctcgagg gcgtgcagcg caacttaggt cagaaggaag agactctcga tcgaaggcaa
     2221 gagcagttgg agcgacgcga ccgcgagctg gattcactgg taacagcgct tggcaacagg
     2281 gagcaggctg ccgctagcaa ggagacacag tgcgcccagt tgatggagga tgcgcagcga
     2341 caactggagc gcatcgccgg cctcacagcc gaagagggta agaagatcct gctccagaat
     2401 ctggagcagg aggccaggat cgagtcgatg gccttgttca aacagattga ggatgagacg
     2461 agggcgacgg caggggagaa ggctaaagag attctggcta ttgccgttga taagtgcgct
     2521 caggagttcg tgatggaatc aacggtttct gtggtggatc tgccgagcga ggagatgaaa
     2581 gggcggatca ttggtcggga gggacgcaac atccgagcct tcgagcgagc cacggggatt
     2641 gatgtgatcg tggacgacac ccccgaagcg gtgatccttt ccggcttcga tccgatccgg
     2701 agagccgtcg ccaaggaggc gctacaacgc ctcatcactg atgggcggat ccatccggct
     2761 cgcatcgagg aggtagtgga gaaggtaaag ggcgagatcg atgatgacat caagaagacc
     2821 ggtgaacgaa cagccttcga actagggatc agcggcatcc accccgagct catgcgcctg
     2881 gttgggcggc tcaagtaccg gaccagctat tcgcagaatc agcttataca caccaatgag
     2941 gtcacacaac tcgctggcgt gatagcggct gagttgggac tcgacgttaa ggttgccaag
     3001 cgggcaggtt tgctccacga tatcgggaag tcagccgacg tcaacatgga agggactcac
     3061 gtctccattt cggccgagtt ggccaagaag tatggtgagc ggccaaaggt ggtgaacgcg
     3121 atcgccgccc atcatgagga cgtggagatc acctgtctgg aggcggtggt actccagatc
     3181 gctgatacgc tctcggcggc caggcccggg gccagacgtg agttgctgga aagttacgtg
     3241 aagcggctcg aggggttaga gaggatcgca aactccttcg cgggtgtgag caagacatac
     3301 gccatccagg ccgggcgcga ggtgcgcatc attgttgaga gcgataaggt atcggacgtg
     3361 caggcgtatc agcttgcgcg ggagatcgcg aagcgaatcg aagaggagat gcagtaccct
     3421 ggtcatatca aagtgaccgt catccgcgag acgcgggcag tggagtacgc caaatagata
     3481 cgttctacgt tcaacgtcac gctcggacgg aagtacaatc ctgaaccttg aacgttgaac
     3541 ttcgaacctg gaacccgatc ccatgcgaat cttattcatc ggtgacatta tcggaaagcc
     3601 gggt
//
LOCUS       DATMXI010000059         3214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_13986, whole genome shotgun sequence.
ACCESSION   DATMXI010000059 DATMXI010000000
VERSION     DATMXI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13986"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..2027)
                     /gene="uvrB"
                     /locus_tag="VN444_01180"
     CDS             complement(27..2027)
                     /gene="uvrB"
                     /locus_tag="VN444_01180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWR20453.1"
                     /translation="MPNFKLVCDYQPKGDQPQAIRKLVDGCLQDRPHQVLLGVTGSGK
                     TFTMANVIAAIERPTLIIAHNKTLAAQLYEEFKAFFPHNAVEYFVSYYDYYQPEAYLP
                     VTDTFIEKDSMINDHIDKLRHSATRSLLERRDVVIVASVSCIYGLGSPEAYYGMMIFL
                     ERGGLCDREQMLRKLVEIQYQRNDYDLHRGSFRVRGDVVEIFPAYADTVVRVELFGDE
                     VEGLWEVDSLTGERIQGLHRVPIYPATHYVTPHDRREEALAAILEELNEQVAAFEKAG
                     KLLEAQRLKQRTFFDLEMIREIGVCKGIENYSRHLSGRTPGEPPPTLMDYLPKDALVI
                     IDESHQSIPQFRGMYHGDRSRKGTLIDYGFRLPSAMDNRPFTFEEFERAVHQVIYVSA
                     TPGPYELAKVKGEIVEQIIRPTGLIDPEIQIRPIRGQIDDLIGEVRAVTARGHRVLVT
                     TLTKRMAEDLTDYLADVGIKIRYLHSDIDTLERNEIIRELRLGKCDVLVGINLLREGL
                     DLPEVALVAILDADKEGFLRSSGSLIQTIGRAARNVEGRVVLYADTVTDSMKRAIEET
                     GRRREVQMAYNLEHGITPESIKKSISDVLSSVSEKDYYTVPATPDVEKAGRLTKEELS
                     AQIEQLEKEMRAAAKRLEFERAAEFRDQIREMEKHHLGIVEG"
     gene            complement(2039..2584)
                     /locus_tag="VN444_01185"
     CDS             complement(2039..2584)
                     /locus_tag="VN444_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWR20454.1"
                     /translation="MDTDKVLVLNNSFEPLHVCAARRAIILLYTGKAERIEDSPSIIH
                     SPSVAFVIPAVIRLYRYVKLPINPTIAFSKKNILRRDAYTCQYCGRNGGERMTIDHVI
                     PRSLGGRTIWENVVSACRACNVKKGNTPLHEARMNLLRKPSRPASIFYLGIMSHSQHR
                     IISWSKYLPREVGGDLQGAGS"
     gene            complement(2708..>3214)
                     /locus_tag="VN444_01190"
     CDS             complement(2708..>3214)
                     /locus_tag="VN444_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HWR20455.1"
                     /translation="EFILARGEMIETIVSTGLGVVKLTEAHLKSDPPTPGELKAVNQV
                     VADRIDRLRAELPDLDGTALIGTAGTPTTLAAIDMGLTVYTPDRVDGHRLSMTRTRQL
                     LHMLATRPLAERSKIPLLEPGRADLIVAGTTLIVTVMGALGYDECMVSDSGLREGVLL
                     DLLRQQTP"
BASE COUNT          660 a          955 c          924 g          675 t
ORIGIN      
        1 tgtaaggcag tggtgagcgc actgccttaa ccctcgacga tgccgaggtg gtgcttctcc
       61 atctcccgga tttgatcccg gaactccgcc gctcgctcga actcgagccg cttcgccgcc
      121 gcccgcatct ctttctccaa ctgctcgatc tgagctgaca actcttcttt cgtaaggcgt
      181 ccggccttct cgacgtcggg cgtggctggg acggtatagt agtccttctc ggagacgctc
      241 gagaggacat cagagattga cttcttgatg ctttccggcg taatcccatg ctcgaggtta
      301 taggccatct ggacctcacg tctccgcccg gtctcctcaa tggcacgctt catcgagtct
      361 gtgacggtat cggcatacag taccacccgt ccctcaacat tccttgcggc ccggccgatg
      421 gtctggatga gggatccgga ggagcgaagg aacccctcct tatcggcatc caagatggcc
      481 accagcgcca cctcagggag atccagtcct tcccgcaaga ggttgatccc gaccagcacg
      541 tcacacttcc caagccgaag ctcgcggatg atctcattcc gctccagggt atcgatgtca
      601 gagtgcaggt agcggatctt gatcccgaca tcggcaaggt agtcggtgag atcctcggcc
      661 atccgcttgg tgagcgtcgt gaccaggacc cgatggcccc tggcagtgac cgcccggact
      721 tcgccgatca ggtcatcgat ctgcccacgg atcggcctga tctggatctc ggggtcgatc
      781 agcccggtgg ggcggataat ctgctccacg atctccccct tgaccttcgc aagttcatag
      841 gggcctggcg tcgccgacac atagatgacc tggtgcacgg cccgctcaaa ctcctcgaag
      901 gtgaacggtc gattatccat agcggacggg aggcggaacc cgtaatcaat cagcgtcccc
      961 ttgcgagaac ggtcgccgtg atacatacca cggaattggg ggatcgactg gtgggattca
     1021 tcgatgatca ccagggcatc cttgggcagg tagtccatca gcgtgggggg cggctcgccc
     1081 ggggtccggc ccgagagatg gcgcgagtag ttctcgatcc ccttgcagac cccgatctcc
     1141 cgaatcatct ccaggtcgaa gaaggttcgc tgcttcagcc gctgcgcctc gaggagcttc
     1201 ccggccttct caaaggccgc gacctgctca ttcagttcct cgagaatcgc agcaagggcc
     1261 tcctctcgac ggtcgtgggg ggtgacataa tgtgtggccg gatagatcgg aacccgatgg
     1321 agtccctgaa tccgctcgcc cgtaagcgag tcgacctccc acaacccttc cacctcgtcg
     1381 ccgaacagct ccacccgaac caccgtgtcg gcgtaggcgg gaaagatctc caccacgtcc
     1441 ccccgcaccc ggaagctgcc gcgatgcaaa tcgtagtcgt ttcgctgata ctggatctcc
     1501 accagcttcc ggagcatctg ctcccgatca cacaagccgc cccgctccag gaagatcatc
     1561 atcccataat aggcttccgg ggagcccaaa ccgtagatgc atgagacaga ggccacgatg
     1621 accacgtcgc gccgctccag cagcgaacgc gtggctgaat ggcgaagctt gtcgatgtga
     1681 tcgttgatca tggaatcctt ctcaatgaag gtatcggtaa caggcaggta cgcctccggc
     1741 tgatagtagt cgtaatagct gacaaagtat tcaacggcgt tgtgcggaaa aaacgcttta
     1801 aactcttcat agagctgtgc cgccagggtc ttgttgtggg caataatcag cgttgggcgt
     1861 tcgatcgcag cgatgacgtt cgccatggta aaggtcttgc cagaaccggt cacccccaga
     1921 agtacctggt gaggcctgtc ctgcaggcac ccgtcaacca gcttcctgat cgcctggggt
     1981 tgatcgccct ttggctgata gtcgcagacg agcttgaaat tgggcatagt cagggcggtc
     2041 aggaaccggc accctggagg tccccgccaa cttcgcgggg gagatacttg ctccaggaga
     2101 tgatccggtg ttgggaatgg gacatgatcc ccaggtaaaa gatagaggcc ggcctggagg
     2161 gtttgcggag cagattcatc ctggcctcgt gaagaggcgt gttccctttc tttacgttgc
     2221 aggcgcggca cgcgctcacc acattctccc agatggttcg tccccccagc gatctgggaa
     2281 tcacatggtc gatggtcatc cgttcaccac cgttgcggcc gcagtactgg caggtatagg
     2341 cgtcccgcct cagaatattt ttcttgctga aggcgatcgt ggggttgatc ggaagcttca
     2401 cgtaacgata gaggcggatc acggcaggaa tgacaaaggc gaccgagggg gagtgaataa
     2461 tgcttggact gtcctcgatc cgttcggcct ttccggtgta cagcagaata atggctcttc
     2521 gcgcggcgca gacgtggagg ggttcaaagg agttattcaa taccaatact ttgtcggtgt
     2581 ccatcgagcg tttcagtagg cttaaggctt gcgaatcctc ctatagtagt tatgatccgt
     2641 agcatacaac ggacacgttg tcaacgtaaa ggaacggaaa aaaggaagga agaatacggc
     2701 gtgagtctca cggggtttgc tgtctgagga ggtcgagaag gaccccttct cgcagaccac
     2761 tgtcgctgac catgcactca tcgtagccaa gcgcgcccat aaccgtcaca atgagggtag
     2821 tccctgcaac aatcagatcg gcacgtcctg gttccaggag cgggatctta ctccgttcgg
     2881 ccagcggtct cgtcgccaac atgtgaagta gttgcctcgt ccgagtcatc gacagccgat
     2941 ggccgtccac tcgatcgggg gtatagacgg tgagccccat atcaatcgca gcgagggtag
     3001 tcggggtccc ggcggtgccg atcagcgccg ttccatcgag atcgggtaat tcagcgcgaa
     3061 gtcggtcaat ccggtccgcg acaacctggt taacggcctt caactcaccg ggggttggag
     3121 gatcgctttt cagatgcgcc tcagtcagct tgactacgcc cagaccagta ctcacgattg
     3181 tctcaatcat ctcccccctg gccaggatga actc
//
LOCUS       DATMXI010000060         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_238311, whole genome shotgun sequence.
ACCESSION   DATMXI010000060 DATMXI010000000
VERSION     DATMXI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238311"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..397)
                     /locus_tag="VN444_01195"
     CDS             complement(26..397)
                     /locus_tag="VN444_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20456.1"
                     /translation="MPHVVIEEAGDLECLYDAFTPIIHRAGSEILKVQEFFLARSGKD
                     ALLDSVAIENGVARTFFVQLKLHEKAITVRLLPATDPEKTPAVKKVMALVAGFIRKVY
                     PGSHYGKTNLQEYLETTTGSK"
     gene            complement(410..1363)
                     /locus_tag="VN444_01200"
     CDS             complement(410..1363)
                     /locus_tag="VN444_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HWR20457.1"
                     /translation="MTTTPLRLGISRCLLGDEVRFDGGHKKDNFLTEVFGRYVEWVPV
                     CPEVEAGLGTPREAMRLVGDPQNPRLVTIKSGADHTRALETLTTNRIAQLMELDLSGY
                     VFKKGSPSCGIERVRLYNEHGMPSRNGVGLFARAFIEEFPLIPVEEEGRLCEPTLREN
                     FIERVFCYRRWQDLVQSGVTRRALVQFHTIHKYLLLAHSPQQYEVLGRLVGQAERHRP
                     KEFAHRYGELFMKALAVKATVRKHVNVLQHIIGHFKERLGAHEKSELLSVIGDYHQEL
                     TPLIVPLTLIKHYVQIFDVGYIRDQVYLNPHPKELMLRNHV"
     gene            complement(1360..>2443)
                     /locus_tag="VN444_01205"
     CDS             complement(1360..>2443)
                     /locus_tag="VN444_01205"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HWR20458.1"
                     /translation="VRTFKDHVVFQAEEVRSATGEPMQRYSAYRARWWTKWQAVKPTA
                     LPVPRFLRAKKAAALPIPPPLPTASDLGYEPVTLWIEPGEQNAQKRLCRFVQGPIHVY
                     ITGRNLPAVDGSSILSPHFRFGTLSARAAVHAALASLGQGRHVSRPDVFTWIDELVWR
                     EFFHQVLAAFPRVVDEPFRQVPAPAPRAPGTERDRFYQAWCEGKTGVPIVDAGMRQLN
                     QTGWMHNRVRMIVASFLVKDLRIDWQSGERYFMQHLIDADVAANNGNWQWCASTGTDA
                     MQGYRIFNPLLQSKKFDPKGEYIRKYVPELATIPTDRIHAPHLMTQEEQASAECRIGT
                     DYPSPIVDHQQARDEYLALGKLQRTR"
BASE COUNT          511 a          710 c          669 g          553 t
ORIGIN      
        1 tgttgatatt cgcgtctgtt cgcactcact ttgaacccgt cgttgtctct agatattcct
       61 gtaagttggt cttgccgtag tgactgcccg ggtacacttt tcgaatgaat cccgccacca
      121 aagccatcac ctttttcacc gcgggggtct tctcaggatc tgtggcaggc aagagtcgga
      181 cggtgatcgc cttctcgtga agcttgagct ggacaaagaa ggtgcgcgca accccgtttt
      241 cgatcgcaac cgaatccagc agcgcgtctt tgccgctcct cgcgagaaag aactcctgga
      301 ccttgaggat ctcactccct gcccgatgaa tgatcggtgt gaaggcatca tataagcatt
      361 ctagatcccc ggcttcttct atgacaacat gcggcatggt tcttcctccc tacacatgat
      421 tgcgcaacat gagctccttg ggatgcggat tgagatagac ctggtcccga atgtacccga
      481 catcgaagat ctggacgtag tgtttgatca gtgtgagagg aacaatcagg ggcgtaagct
      541 cctgatgata atcgccgatc acgctcagca actcagactt ttcgtgggcg ccgagccgct
      601 ccttgaaatg tccaataatg tgttggagca cgttcacatg tttgcgtacc gtcgccttca
      661 cggccaatgc cttcatgaac agttccccat accggtgagc gaattccttg ggacggtgcc
      721 gctctgcctg accgaccagc cgcccgagca cctcgtattg ctgtgggcta tgagctaaca
      781 gcaaatattt gtggatcgtg tggaactgca ccagggcccg cctcgtgacg ccactctgta
      841 cgagatcctg ccagcggcgg taacagaaga cccgttcgat aaaattctct ctcagtgtag
      901 gctcacagag ccgtccttct tcctcgactg gaatcagcgg aaactcctcg ataaaagccc
      961 gcgcaaagag tccgactcca ttccggctgg gcatcccatg ctcattgtag agacgaactc
     1021 gctcgatgcc gcagcttggt gaacctttct taaagacata cccggataag tccagctcca
     1081 tgagttgcgc gatacgattc gtggtcaacg tttccagtgc gcgcgtgtga tcagccccac
     1141 tcttgatcgt cacaagtcgg ggattctgag gatcgccgac cagtcgcatc gcctcgcgag
     1201 gagtgcccag tcctgcctca acctccggac agaccggaac ccactccaca tagcggccaa
     1261 acacttcggt cagaaaattg tccttcttat gtcccccatc gaaccggact tcgtctccga
     1321 ggagacaacg gctgattcca aggcggagcg gcgtggtcgt catctcgtcc tctgtagttt
     1381 gccgagggcc aaatattcat cgcgggcttg ctggtgatcc acgataggcg aagggtagtc
     1441 tgtaccaatg cggcattcgg ccgaggcctg ttcttcctgg gtcatcagat gtggcgcatg
     1501 aatcctgtca gtcggaatag tcgctagctc cggcacatac ttccgaatgt attcgccttt
     1561 tggatcgaac ttcttgctct gtaacagagg attgaaaatc ctgtatcctt gcatcgcatc
     1621 cgtgccggtc gaggcgcacc actgccagtt cccattgttt gctgcaacgt cggcgtcgat
     1681 gagatgctgc atgaaatacc gttcgccgct ctgccagtca atccgcaggt ccttgacgag
     1741 aaacgaggcg acgatcatcc gcacgcggtt gtgcatccat ccggtctggt tcaactggcg
     1801 catgcccgcg tccacgatcg gaacgccggt cttaccctcg caccaggcct gatagaatcg
     1861 atcgcgttcg gtgccaggcg cacgtggagc cggtgccgga acctggcgaa atggctcgtc
     1921 gacgacgcga gggaaggcgg ccagaacctg atggaaaaat tcccgccaga ctaattcgtc
     1981 gatccaggtg aagacatcgg gacgagagac gtgtctgccc tgtcccaacg atgccagggc
     2041 cgcatgtact gcggcgcggg ccgacagggt cccgaatcga aagtggggcg agagaatcga
     2101 actgccgtcg acagcgggaa gatttcgccc cgtgatatag acgtgaatgg ggccttggac
     2161 gaaccgacat aatcgttttt gagcgttttg ttctcccggc tcgatccaga gcgtcaccgg
     2221 ctcatatccc aagtcgcttg cggtgggcaa cggaggcgga atgggcaatg cggcagcctt
     2281 tttagccctc aagaaccggg gaactggaag ggcggtcggt ttcactgctt gccacttcgt
     2341 ccaccagcgt gcacgatagg cgctataccg ctgcatcggc tcgccggtcg cgcttcgaac
     2401 ttcttcggcc tggaagacca catggtcttt gaatgtccgg acg
//
LOCUS       DATMXI010000061         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_172592, whole genome shotgun sequence.
ACCESSION   DATMXI010000061 DATMXI010000000
VERSION     DATMXI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172592"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VN444_01210"
     CDS             complement(<1..340)
                     /locus_tag="VN444_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWR20459.1"
                     /translation="MIEEFLSSWPLFHNTYLVGWSIALLLSLVGVLAVARNQIFIGAA
                     ISQASTLGIALAMWIGAWVGPESPSWLRLDGFLSVMAVAFSVIAALITARGGEESRES
                     HEAITGWVFLV"
     gene            complement(337..1107)
                     /locus_tag="VN444_01215"
     CDS             complement(337..1107)
                     /locus_tag="VN444_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR20460.1"
                     /translation="MENTDVALKATGLTVGYARRTVLQNVTLECRKGEFWFFIGPNGS
                     GKTTLIRTMLGILRPQAGGLWLHPDLAHRKGLGFVPQRCDLNPALPTTVREFVILGLV
                     GIRLGTKEEAERLEWALRKVGLEGMAGRDYWSLSGGQRQRALVARALVRRPGLLILDE
                     PTNGLDLSTEDSFLRLLADLNREEHLTLFFVTHDIAIAARYATHLALFRSGSVEPGSR
                     QQMLNRAALERVYGVGVEVIRDPSGAVAVHVYPPGEHP"
     gene            complement(1164..2225)
                     /locus_tag="VN444_01220"
     CDS             complement(1164..2225)
                     /locus_tag="VN444_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWR20461.1"
                     /translation="MFNRLRRLFRLSVMGWLVASFGLAGSSAFAQEGTKPIAVCATVP
                     DLGSLAREVGGDQVSVTVFAKGTEDAHFIEAKPSFIKALSQCDLYIQMGMDLEIGWAP
                     VLLQNARNGAVLPGGRGYIDASRAILRLEVPTGQVDRSMGDVHPLGNPHYLLDPLNGL
                     KVARVIRDKLVELRPDRKPYFEDRFISFHLRLGTALVGEKLAEKYDTEKLALLYEHGK
                     LATFLKGQGEASLLQGWLGRMLPHFGTKVVADHNLWPYFARRFGIAVIGFMEPKPGIP
                     PTTKQLGTLVDLMRAERVGAVLTVSYYDPRHARFISQQTGTRVVNLAHQAGARDGTDN
                     YLAMIDYNVREMAAALEGA"
BASE COUNT          476 a          727 c          703 g          442 t
ORIGIN      
        1 atacaaggaa tacccatcct gtgatcgctt catggctctc tctcgactct tcgcctccgc
       61 gcgcggtgat cagagccgcg atcacggaga aggccacggc catgacggac aggaacccat
      121 ccaagcgcaa ccaggacggg ctctccggac cgacccacgc cccgatccac atcgcaagag
      181 cgatgccaag ggttgatgcc tgagaaatgg ccgcgccgat gaagatctgg ttacgggcga
      241 ctgcaagcac tccaaccaac gagagcaaca gggcgatcga ccagccgacg aggtacgtgt
      301 tgtgaaacag aggccatgat gagaggaact cctcgatcac ggatgttctc ccggggggta
      361 gacatggact gcgacagcgc ccgatgggtc gcggatcacc tccacgccca ccccgtacac
      421 ccgctcgaga gcggcccgat tgagcatctg ttggcgcgat ccgggctcga cgctacccga
      481 acggaagagc gccaggtgcg tggcgtatcg cgcggcaatg gcgatatcgt gggtgacgaa
      541 gaagagggtc agatgttcct cccggttcag gtcggcgagc aatcgcagaa atgagtcttc
      601 cgttgagagg tcgagaccat tggttggttc gtcgaggatc agcaggccag gccgtcgcac
      661 gagggctcga gccaccagcg ctcgttgtcg ctgcccgccg gaaagggacc agtaatcgcg
      721 ccccgccatc ccctccagac cgaccttgcg gagcgcccac tcgaggcgct ccgcttcctc
      781 tttcgtgccg agccgaatgc ccacgagccc cagtatgacg aattcccgga ccgtggttgg
      841 gagggctgga ttcagatcgc atcgctgcgg gacaaaaccg aggcccttcc gatgcgccag
      901 gtccgggtgc agccataatc cgccggcctg gggtcgaagg atccccagca tggtgcgaat
      961 cagcgtagtc ttgccggagc cgttcggtcc gataaagaac cagaactccc ccttccggca
     1021 ctcgagagtg acgttttgga gtacagtccg gcgcgcgtag ccgacagtca gccctgtcgc
     1081 tttcagtgca acatcagtgt tctccatgtt cctccggaaa tagcggtcag caatcaggga
     1141 tttaggctga aggctgaagt accctatgcg ccttccaagg ctgcggccat ctctctcaca
     1201 ttatagtcaa tcatggcgag gtagttatcg gtaccatcgc gcgcccctgc ctggtgggcc
     1261 aggttcacga ccctggtgcc ggtctgttgc gagataaatc gggcatggcg agggtcatag
     1321 tagctcaccg tcagaaccgc cccgacccgt tccgctcgca ttaggtccac cagcgttccc
     1381 aactgcttgg tcgttggggg gattcctggt ttcggctcca tgaacccgat gacggcgatc
     1441 ccaaaccggc gagcgaagta gggccacaaa ttgtgatcgg ccacaacctt ggttccgaag
     1501 tgaggaagca tcctccccag ccagccctgc aggagcgatg cctctccctg tcccttcagg
     1561 aaagtggcaa gcttgccgtg ttcgtagagt agggcgagct tttcggtatc atacttctcg
     1621 gcgagcttct cgcccactaa tgctgtaccc agccttagat gaaaagagat gaagcgatcc
     1681 tcgaagtatg gcttcctgtc cggtcgcagt tcaaccagct tgtcgcggat caccctcgcc
     1741 actttgaggc cattcaatgg atcgagcaga tagtgtgggt tgcccagggg gtgaacatcg
     1801 cccatcgagc ggtccacctg gccggtgggg acttccagac gcaagatggc cctggaggcg
     1861 tcgatgtagc cgcgcccacc cggcaggacc gccccgttcc tggcgttctg tagcaggacg
     1921 ggagcccaac cgatctccaa gtccattccc atctggatat agagatcgca ctgactcagc
     1981 gccttgatga agctgggctt ggcctcgata aaatgcgcgt cttccgttcc cttggcgaaa
     2041 accgtgaccg agacctggtc tcctcccacc tcgcgggcga ggctccccag gtccggtacg
     2101 gtagcgcaca ccgcaatcgg cttggtgccc tcttgggcga aggcggatga gccggccagg
     2161 ccgaaggagg ccacaagcca gcccatgacg ctcaggcgaa ataagcgacg caatctattg
     2221 aacatagccg atcctccttt ggccctggtt agtatttgtg ggcggggtgg gcgccgtaga
     2281 ggaactcgac ccccaaccag acggaatgag catcctgtcg ttccaggtga tcggcccggt
     2341 cgtagttg
//
LOCUS       DATMXI010000062         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_211601, whole genome shotgun sequence.
ACCESSION   DATMXI010000062 DATMXI010000000
VERSION     DATMXI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211601"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VN444_01225"
     CDS             complement(<1..898)
                     /locus_tag="VN444_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWR20462.1"
                     /translation="MDPRAQVSMVFHLDKCIGCHTCSVACKNIWTDREGAEYMWWNNV
                     ETKPGTGYPTRWEDQERYKGGFEQNGDELRLRLQGRLGALANIFFNPYLPTVDEYYEP
                     FDYDYEKLFSAPEGDDQPAVRAKSMISGEYMEIEAGPNWDDDLGGSPVYAKNDPNLKN
                     LTEAERASLNEIERMVFFYLPRICNHCANPGCVAACPAGAIYKRGEDGIVLIGQDKCR
                     AWRMCISGCPYKKPYFNWKTGKSEKCILCYPRLESGQPPACFHSCVGRIRYLGVLLYD
                     ADQIRSAAGVPDDRLVEAQREMV"
     gene            complement(923..1504)
                     /locus_tag="VN444_01230"
     CDS             complement(923..1504)
                     /locus_tag="VN444_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20463.1"
                     /translation="MQDAWVEQMEQTLNPMSVPMDNPIGTIESKDRQAILAALLVHLW
                     HGYRKMRDCLMELEDAMLQGNREQARSLLRQIMRFCGTSFRYEEDAVLPALDHHIGSE
                     QLHELTAAHDLVIRHVRRLEELLSTSPDGEEQIEQGRPLIHALLMQVACTAGLTLLIE
                     TLPQDALIRILQARERALIDGKDLYEWDKDIRG"
     gene            complement(1512..>2419)
                     /locus_tag="VN444_01235"
     CDS             complement(1512..>2419)
                     /locus_tag="VN444_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWR20464.1"
                     /translation="RTVSWGGQKYPSLETPLDAANLILHLSPETNGEICYEGFKAKGE
                     LVGVELADLAEGHRSQRLNFGDLKRQPRRTLISPCWSGMTNDGRAYSAWTMMVERLVP
                     WRTLTGRQHLYLDHPYFIDFGENLPTYKPKLDPVLIHDITESGQDERALLLNYITPHG
                     KWNIHSTYKDNHRMLTLSRGMDPIWINDKDAARVGLKDNDWVEVYNDNGVVVTRTNVS
                     SRVQSGTCLYYHAVERTIYVPKSQLRGRRAGGHNSLTRTRVNPVQLAGGYGQWSYGFN
                     SLGPIGIMTRNTYCLVRKMDKLQWE"
BASE COUNT          450 a          725 c          706 g          538 t
ORIGIN      
        1 gcaccatctc tcgctgggct tcaaccaggc gatcatcggg gacgccggct gccgatcgta
       61 tctgatccgc atcgtagagg agcacaccca gatagcgaat ccggcccacg cacgaatgaa
      121 aacaggcggg gggctgtccg ctttccagcc tggggtaaca cagaatgcac ttctcggact
      181 tcccggtctt ccagttgaaa tacggtttct tatagggaca gcccgagatg cacatcctcc
      241 aggcgcggca cttgtcctgg ccgatgagca cgatcccgtc ctctccccgt ttataaatgg
      301 cgccggccgg acaggcggcc acgcatccgg gatttgcaca gtggttacag atgcggggca
      361 gatagaagaa gaccatccgc tcgatttcgt tcagcgacgc tcgctccgcc tcggtgaggt
      421 ttttcaggtt gggatcgttc ttcgcgtaca caggggaacc gcccagatca tcgtcccagt
      481 tagggccggc ctcgatctcc atgtattcgc cggagatcat tgatttcgcc cggaccgcgg
      541 gctgatcgtc cccctcgggc gccgaaaaca gtttctcgta atcgtaatcg aacggctcgt
      601 aatactcgtc gacggtaggc aggtacggat tgaagaagat gttggccagc gcgccgagcc
      661 ggccctgcaa tctgagtctc agctcgtccc cattctgctc aaatccgcct ttataccgct
      721 cctgatcctc ccagcgggtc gggtagccgg taccgggttt ggtctccaca ttgttccacc
      781 acatgtattc ggccccttcc cgatcggtcc agatgttctt gcaggccacg ctacacgtgt
      841 ggcacccgat acatttgtcc aggtggaaga ccattgatac ctgtgcgcga ggatccatta
      901 ggttcgcctc cagaattgct ctctagccac gaatgtcttt atcccattca tacagatctt
      961 tcccatcgat cagggcgcgc tcacgggcct gcaggatccg aattaatgcg tcttgcggta
     1021 acgtttcaat caacagcgtc aggcctgcgg tgcaggcaac ctgcatcagc aatgcgtgaa
     1081 tcagcggtcg accctgttca atctgttctt ctccgtcggg agacgtcgag agcagttcct
     1141 ccagccggcg aacgtgccga atcaccagat cgtgggctgc cgtcagttca tgtagctgtt
     1201 cactgccgat gtgatgatcg agcgcaggca ggacagcatc ctcttcgtag cgaaaagacg
     1261 tcccgcaaaa tctcatgatc tgccgaagga gcgaccgggc ctgctcgcga ttgccctgca
     1321 gcatggcgtc ttccagctcc atcaggcaat cccgcatctt gcggtagcca tgccagaggt
     1381 gtacgagcag ggcggccagg atcgcctgac gatccttcga ttcaatggtg ccgatgggat
     1441 tatccatcgg gaccgacatt ggattcagcg tctgctccat ctgctcgacc caggcgtctt
     1501 gcatcagcag tttactccca ttgcagtttg tccattttgc gcaccagaca gtaggtgttg
     1561 cgcgtcatga tcccgatcgg tcccagtgag ttgaatccgt acgaccactg accgtatccg
     1621 ccggcgagct ggaccgggtt cacccgcgtg cgggtgaggc tgttgtggcc gccggcccgc
     1681 cgtccgcgaa gctgagactt cggtacgtag atcgtccgtt cgaccgcgtg ataatacagg
     1741 caggtgccgg actggacgcg ggagctgaca ttggtccggg tgaccacgac gccgttgtcg
     1801 ttgtagacct ccacccagtc gttgtccttg aggcccacgc gggcggcgtc tttatcgttg
     1861 atccagatcg gatccatgcc ccgcgacaaa gtgagcatgc ggtgattgtc tttgtaagtg
     1921 gagtggatgt tccacttgcc gtggggcgtg atgtagttca ggagcagggc gcgttcatcc
     1981 tgtccgcttt cggtgatgtc atggatcagg accgggtcga gcttcggctt gtaggtcggc
     2041 aggttctcgc cgaagtcgat gaaatagggg tgatcgaggt acagatgctg gcggccggtc
     2101 agcgtgcgcc agggcacaag ccgctccacc atcatcgtcc aggccgaata ggcgcgtccg
     2161 tcgttggtca tcccgctcca gcagggagag atcagcgtgc gcctgggctg ccgtttcaga
     2221 tcgccgaagt tcagccgttg gctgcggtgg ccttcggcca gatcagcaag ctctaccccg
     2281 acaagctccc ctttggcctt gaagccttcg tagcagattt cgccgttggt ctcgggcgac
     2341 aggtgcaaaa tcaggttggc ggcatccagc ggcgtttcaa gcgaggggta cttctgtccg
     2401 ccccatgaga ccgtccgct
//
LOCUS       DATMXI010000063         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_194047, whole genome shotgun sequence.
ACCESSION   DATMXI010000063 DATMXI010000000
VERSION     DATMXI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194047"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VN444_01240"
     CDS             <1..852
                     /locus_tag="VN444_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWR20465.1"
                     /translation="PSGIEAHRIVAKLDAVGNTTKALTKGLAIATAVIAAVSLFRSFI
                     DEAHLLETGIQVNLPEVFIGFLIGGAVPFLFCSFAIQAVSRAAFLLVEEVRRQFREKP
                     GIMEFKEKPDYGRCVEIVTAAAQKELLGPGVLSIVSPILVAFAFGAPALGGFLAGAIL
                     TGQLMAVFLSNTGGAWDNAKKKIEEGLFGGKGTDCHKAAVIGDTVGDPFKDTAGPAIN
                     PMIKVMNLVAILIAPLAIREIGFGTRAMIVLACATILGLSVLFSKRGSIVEEEPAVDK
                     AEVSRAH"
     gene            1188..1811
                     /locus_tag="VN444_01245"
     CDS             1188..1811
                     /locus_tag="VN444_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20466.1"
                     /translation="MGFLSDLFSRQSDDERQLEATHARMFQSMMGLSASEARRIARDM
                     IHDAKEELKRSEAPDMAQDIGDQMLQRESTDPSVQEQLAKKRQEGVTDADIRWWWNLP
                     ALERVLIEKVDGLERYTICLKWSGEGHTPGQAAATLRKLHPMYGDPADTSETTGDDRP
                     LPYELRNRVNSYIQKRSQDDPLAYKKDIAHSSTFNALVRRGIRSGQI"
     gene            2020..>2471
                     /locus_tag="VN444_01250"
     CDS             2020..>2471
                     /locus_tag="VN444_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20467.1"
                     /translation="MKIMPLLFSTLRRCPVLDAEGQLVGHLKDLILLPLGVLPPVTKL
                     VVLTSEKDELVLPWEAVARIEREPAAIHLGQAGEALQPVPLRPEEMELAQSLLDRKVV
                     DTKHCEVIRVNDLEFQEAEGRLELMAVEAGLRGLLRHLGSEAMAERIA"
BASE COUNT          497 a          687 c          792 g          495 t
ORIGIN      
        1 ccctcgggaa tagaggccca tcggattgtc gccaagctcg acgcggtcgg caatacgacc
       61 aaggccctaa cgaaagggct ggccatcgcc accgcggtca tcgcggccgt ctccctcttt
      121 cgctccttta tcgacgaggc gcacctctta gagacgggga ttcaggtgaa cctgccagag
      181 gtcttcatcg gcttcctgat cgggggtgcg gttccgttcc tcttctgctc ctttgcgatc
      241 caggcggtaa gccgagcggc cttcctcctg gtggaggagg tccggcggca gttccgggag
      301 aagccgggga tcatggaatt caaagagaag cccgactacg gccggtgcgt ggagatcgtg
      361 acggctgcgg cgcagaagga actgctggga ccgggcgtcc tctcgattgt cagcccgatc
      421 ctggtcgcgt tcgccttcgg cgcaccggcg ctgggtggct tccttgccgg cgcgatcctc
      481 accgggcaac ttatggcggt gttcctgtcg aataccggcg gggcatggga caatgccaag
      541 aagaagatcg aggaggggct gttcggcggc aaggggactg actgccacaa ggccgcagtc
      601 atcggcgata cggtcggcga tccgttcaag gacaccgccg ggccggccat caacccgatg
      661 atcaaggtca tgaacctggt cgcgatcctg atcgcaccct tagccatacg cgaaattggg
      721 tttggcactc gggcgatgat cgttctggcc tgcgcgacca tcctggggct gtcggtactc
      781 ttcagtaagc gaggctcgat cgtggaggag gagcccgcgg tggataaggc ggaggtcagc
      841 cgcgcccact aggccacaga actggcgccc atcctatgcg gcaggtgtgc tgcgttcctg
      901 cctgcgaata aaccagggca gtgcgctagg cgtcccttcc ttttgattcc gttgagggtc
      961 gataccccgc ggcttgccgc gagttcgtca taccggcgga agccggtatc cagagggcct
     1021 gactggattc cagtccttct gacctggatt ttcgcttttg atggaagtac tcgcgctata
     1081 tctgcacgct ctgccggtca gcacgctcaa taggtcgccg ccttttcggt atggtatgct
     1141 gtggaaagct tcgttgtggt ttggaaaaac catagggagg gttacccatg ggctttctct
     1201 ccgatctctt ttcacgccag tcagatgacg aacgccagct tgaggcaact catgctcgga
     1261 tgtttcagtc gatgatggga ctgtcggcca gtgaggcacg acgcatcgct cgagacatga
     1321 tccatgatgc gaaagaggag ttgaagcgat cggaagcccc tgacatggcg caagatatcg
     1381 gcgatcagat gcttcaacga gaatcgacgg atccgtccgt tcaagaacaa cttgccaaga
     1441 agagacagga aggtgtgacg gatgccgata tacggtggtg gtggaatctc cctgcccttg
     1501 agcgagtact catagaaaag gttgacgggc tcgagcggta taccatttgt ctcaaatggt
     1561 cgggggaggg tcacacgcca gggcaagcag ccgccacact caggaagctt cacccgatgt
     1621 acggcgatcc ggctgatacg tctgagacta caggggatga tagaccgttg ccatacgagt
     1681 taaggaatcg agtgaacagc tacatccaaa aacgatccca ggacgatccg ctcgcctaca
     1741 aaaaggatat tgcacactct tctaccttca atgcgctcgt ccgtaggggc atacgaagcg
     1801 gtcagatatg aagggtgggg gagaggatca aagacccgcg gcgaacaatt cgctccacgg
     1861 gccgcgtccc cggcctctcg ccttcgatcg agccctgtga cgcgcccgtg agtttagccg
     1921 attcggcggc aatgggatcg actgccacaa gactgcggtc atcggcaata cggttgacaa
     1981 agggtccggt tcatactagg agaataagaa ggattccgta tgaaaatcat gcctctcctc
     2041 ttctccacgt tgcgacgctg tccggtcctc gatgccgagg gacagctcgt gggtcacctg
     2101 aaggacctga tcctcttgcc attgggggtc ctgcccccgg tgaccaagct cgtggtcctg
     2161 acatccgaga aggacgagct ggtcctgccg tgggaggccg tggcgcgcat agagcgggag
     2221 ccggcggcga ttcacctggg tcaggcgggt gaggcgcttc agccggtgcc gctgaggcct
     2281 gaggagatgg agttggcgca gagcctcctg gaccggaagg tggtggatac caagcactgc
     2341 gaggtaatcc gggtcaacga tcttgaattc caggaggcgg aagggcggct ggagcttatg
     2401 gccgtcgagg ccggtttgcg tggcctgctc aggcatctcg gttccgaggc aatggctgag
     2461 cggatcgcaa g
//
LOCUS       DATMXI010000064         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_216753, whole genome shotgun sequence.
ACCESSION   DATMXI010000064 DATMXI010000000
VERSION     DATMXI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216753"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="VN444_01255"
     CDS             <1..1230
                     /locus_tag="VN444_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20468.1"
                     /translation="LWNNLGVQQEKFGGSALSVKAFKKAVASDPANPLAYTNLTQAYW
                     ELRDPAMTSQFLNRAIRLNPADPFPHLALAELLLEKGDKTSAITHLNIADTQARYDPH
                     LRSYRNTLVAKTNTGNSLQPLAVAKALSPSVKPAADDPLSTPNPRGNLAPPPSMPSAD
                     PPPASITTQVEPSQKPSSHRDSAHFVVQFDGPDDQETWVRIRAILEYAYEEIPPKFGQ
                     VPARPVKVVLHTGQKFSGPAGTPHLADTLFDQKSGSIHFPTEGALDDLALFSRVVRHQ
                     FVHALFHEQAKQNTPAPPTWLMEGLAIHLAEDPWPDIEESRQLNPALMPLPSLHGPWV
                     RLPSTSLPTAYLTATAAIRYLVDRHSMYTIRQLMTVLLAGQPLERAMQQKLSLSYEQF
                     QRQSAESHKHHENERES"
     gene            complement(1270..1941)
                     /locus_tag="VN444_01260"
     CDS             complement(1270..1941)
                     /locus_tag="VN444_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20469.1"
                     /translation="MSVRCVTRLAQVGPVVACTVAGLVTVFGVQSVLAIDVKVSIEEA
                     QKALEAGRAPMEKANTPEEVKKVLQQASMVTRVGAHPEKDPCGASAILRTKRYRLEAF
                     GRQEAAESKKRKMDVRMPDEFIRKVVDMPNMEIEVQLCGDDEYFAEGALIELQQGSKR
                     IKPIDIGKAERGRKNEGSGPIYRSRFTALFAYETFDPTAASTFVINLQDGSEARIAAD
                     FSKVK"
     gene            complement(2010..>2328)
                     /locus_tag="VN444_01265"
     CDS             complement(2010..>2328)
                     /locus_tag="VN444_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWR20470.1"
                     /translation="ALVGSTQKVEVTALSTAVVRMRSFDAQAHERYLETEESLGKAGA
                     YSIQGEGGDLVDSIDGDFPTVVGLPLRLVAQLLRQCGIEVPVDLDELYASKPYANWDR
                     FSG"
BASE COUNT          550 a          741 c          537 g          500 t
ORIGIN      
        1 ctctggaata atctgggcgt acaacaggaa aagttcggcg ggagcgcact ctctgtcaaa
       61 gcctttaaaa aagccgtcgc gtccgatcct gccaatccgc ttgcatacac gaacctgaca
      121 caagcgtact gggaactccg cgacccggca atgacatctc agttcctcaa cagggcaatt
      181 cgactgaacc ccgccgatcc gttccctcat cttgccctcg ctgaactcct attggagaaa
      241 ggcgacaaga cctcggcaat aacccatctc aatatcgcgg atacacaggc ccgatatgat
      301 ccccatctcc ggtcttatag aaatacgctg gtcgcaaaaa cgaataccgg gaactccctg
      361 caacctctcg cagtggcaaa agcactctcc ccctcagtca aaccagcagc agatgatccc
      421 ttatcaactc cgaatccccg tggaaacctc gcacctcctc cctcaatgcc atccgctgat
      481 ccaccgcccg catccatcac gactcaggtg gaaccatcgc aaaaaccgtc atcccatcga
      541 gactccgctc atttcgtcgt tcaattcgac ggccctgacg atcaggaaac gtgggtgcgc
      601 atccgtgcca tactcgaata cgcctatgag gagatccctc cgaagttcgg acaagtaccc
      661 gccagaccag tgaaggttgt cctccataca ggacagaaat tttccggtcc ggcgggaacc
      721 ccccatttgg ccgatacctt gttcgatcag aagtctggtt cgatccattt ccccactgaa
      781 ggcgcactcg acgatctcgc attgttcagc cgcgtggtcc gtcatcaatt tgtgcatgcc
      841 ctctttcacg aacaggctaa acagaacacc cccgctcctc caacatggct catggagggt
      901 ctggccatcc atctggcgga ggacccctgg cccgacattg aagagtcccg acagcttaac
      961 cctgccctca tgcctctgcc ttccctgcac ggaccatggg ttcggctccc ctcaacgtca
     1021 ttaccgaccg catacctcac ggcaaccgct gccattcgat acctcgttga tcgacatagc
     1081 atgtacacca tccgccagct gatgactgtg ctcctggccg gacagccgct agagagggca
     1141 atgcagcaga agttgtccct gtcgtacgaa cagtttcaac gccagtcggc ggaaagccac
     1201 aagcaccacg agaacgagcg cgagtcctaa tgatttaatt aatcggtcgt cagagagcga
     1261 aagaagtgtc tacttcactt tggagaaatc agcggctatc ctcgcttcac ttccgtcctg
     1321 cagattaatg acgaaagtgg acgcggcggt gggatcaaag gtttcatatg caaacagagc
     1381 agtaaaacgc gaccggtaga tcggaccgct tccctcattc tttcgaccac gttcagcttt
     1441 accgatgtcg atgggtttga tcctcttgga gccctgctgc agctcgatca aggcgccttc
     1501 tgcaaagtat tcgtcatcgc cacagagctg aacttcaatc tccatgttgg gcatgtcgac
     1561 gactttcctg atgaattcgt ctggcatccg cacatccatt ttcctcttct tggactccgc
     1621 cgcctcctgt cggccaaagg cttcaagtcg atagcgtttg gtccggagaa tagcgctggc
     1681 tccgcaaggg tctttctctg gatgagctcc tacacgcgta accatggaag cttgctggag
     1741 aactttcttg acctcctctg gggtgttggc cttctccatg ggagcccgcc cggcttccaa
     1801 ggccttctgc gcctcttcta ttgaaacctt cacatcgatc gccaagaccg actgaacacc
     1861 aaagactgtc accagacccg caaccgtaca ggcaacaacg gggccgactt gcgccaacct
     1921 tgtcacacat cgcacactca agactccctc catcagcgag acagcgatca atgagtcggc
     1981 attgtacgaa agcccccatc cctttgcaat cagcctgaaa agcgatccca gttagcgtag
     2041 ggtttgctgg cgtagagttc gtcgagatca actggcactt ctataccgca ttgcctaagc
     2101 agttgagcga ccagacggag gggcaaaccg actaccgttg gaaaatctcc atcaatggaa
     2161 tcgaccaaat cgccgccctc tccttgaatc gagtatgcac cggctttgcc gagactttcc
     2221 tccgtctcca gatagcgttc atgcgcctgt gcatcaaagg acctcatccg aacgacagcg
     2281 gtagacagag cggtaacctc gaccttctga gttgaaccga cgagcgcg
//
LOCUS       DATMXI010000065         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_80095, whole genome shotgun sequence.
ACCESSION   DATMXI010000065 DATMXI010000000
VERSION     DATMXI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80095"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VN444_01270"
     CDS             <1..494
                     /locus_tag="VN444_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20471.1"
                     /translation="LLRNVATSLLIVPVLLAAACAKPVAHQAVPMDFSPPPPGSQPGD
                     RHVLAGEWEYVDTDGAVDRVTLDGEGNGRYNWKDGRFETYTFIGHTWQGKWYQKENNR
                     DGGFAVEFSPDFSEGEGRWWYSRIGADQAPTLKGGTFHLRKKASRTHRSDTLRNRGKH
                     ITP"
     gene            518..832
                     /locus_tag="VN444_01275"
     CDS             518..832
                     /locus_tag="VN444_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWR20472.1"
                     /translation="MKGRKHTRFAVQFPVSFKGDQLSGEGTILNVSGEGCAITAETIA
                     DVAAYLQMMMQLRAGEEPIQVDLAAVRWSSATRFGVEFIKIRPEEEGRLKNFVKALES
                     TT"
     gene            complement(862..>2589)
                     /locus_tag="VN444_01280"
     CDS             complement(862..>2589)
                     /locus_tag="VN444_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWR20473.1"
                     /translation="IEPRAYDALIQEGLIRQTDLVKAIQESLQGATDLEGLLLEKYRI
                     PKAALGKTLSAFFDCPYIPYDERTLADPQLLNNLSKDYLRRHHWLPLKQQGDILDVLI
                     DNPHDLDRGHDIRRSFPGMTIRYALGLRCDIERFLSDTIGDTDCGSIADTLGELVHDA
                     YQEQSLDVNSDGIDENDSAIVRLANQIIAEAFRHDASDIHIEPYSDRKETAIRFRVDG
                     TCSTYMKIPAIYRRALVSRIKIMSSLDISERRKPQDGKIRFKMSQDREIELRVATLPT
                     AGNNEDIVMRLLSAKDPLPLDAMEFAPATLTKLLEIAEKPHGIILCVGPTGSGKTTTL
                     HALLRHLNTDERKIWTAEDPIEITQEGLRQVQVHPKIGFTFASAMRSFLRADPDVIMI
                     GEMRDRETADVAIEASLTGHLVLSTLHTNGAVETVVRLLDLGCDSFNFADAMQGVLAK
                     RLCKRICVTCKEPYHLTAQEYDKLVHGYGAQAWETLGLSYGENFQVYRGRGCSTCNQT
                     GLKGRVALHELLCGSDELRNLIQNRAKTSEMQSLAAKEGMITLVQDGILKVLAGLTTY
                     EQVRSVAMK"
BASE COUNT          536 a          697 c          757 g          599 t
ORIGIN      
        1 tgcttctacg aaacgtcgcg acctctctcc ttattgttcc cgtgttgctc gccgcagctt
       61 gcgccaagcc ggtggctcat caggctgttc ccatggattt ctctcctcca ccgcctggct
      121 ctcagcccgg tgaccgccat gtcctcgccg gtgaatggga atatgtcgat acagatggtg
      181 cggtcgatcg agtaacactc gacggggagg gaaacggtcg ctataactgg aaggatgggc
      241 gcttcgagac atatacattc atcggtcata cctggcaagg taagtggtat cagaaggaga
      301 ataaccggga cggagggttt gcagtcgaat tctcgcctga tttttcagaa ggggagggtc
      361 gctggtggta cagccgcatc ggggccgatc aggcaccgac gctcaagggc ggaacctttc
      421 atctccgaaa aaaggcttct cgtacgcatc gcagcgatac tctgcggaac cgtgggaaac
      481 atatcacgcc ataggctgat gaccagcagg gagcctgatg aaaggacgta aacatacccg
      541 attcgcagta caatttcctg tctcgttcaa gggagatcag ctgtctggcg agggtacgat
      601 cttgaatgtg tcgggcgagg gttgtgccat cactgctgaa accattgccg acgttgctgc
      661 ctatctacag atgatgatgc aactccgggc gggagaagag ccgatccaag ttgatctggc
      721 cgcggtccga tggtcatctg caacaaggtt cggtgttgag tttatcaaga ttcgtcctga
      781 agaagaggga cggctgaaga actttgtcaa ggcactggaa tctacgacct gagggcgtcg
      841 accacggcga gatatggccg gctatttcat ggcgacggag cggacctgtt cataggtggt
      901 caggcccgcg agcactttca gaatcccgtc ctgcacaagt gtaatcattc cttccttcgc
      961 agccaggctc tgcatttccg atgtcttggc ccggttctgg atcaggtttc gtagttcatc
     1021 cgatccgcat aggagttcgt gcaaggcaac ccgacctttg aggcctgtct ggttgcaggt
     1081 gctacaccca cgccctcggt agacctgaaa gttctcgcca taactcagtc cgagtgtttc
     1141 ccaggcctgc gcaccgtatc cgtgcaccaa tttatcgtac tcctgcgccg tcaaatggta
     1201 tggttctttg cacgtgacac agatccgctt gcacaggcgt tttgccagca ccccttgcat
     1261 ggcatcggca aagttgaagg agtcgcagcc caggtcgagg agacggacca cagtctcgac
     1321 cgccccattt gtgtgtaacg tactcaagac caggtggccg gtcagcgagg cctcgatggc
     1381 cacatcagca gtttccctat cccgcatctc accgatcatg atgacatccg ggtcggctcg
     1441 caagaacgat cgcatggcgg acgcgaaggt aaacccaatc ttgggatgga cctgcacctg
     1501 gcgaagccct tcctgagtga tctcgatggg gtcttccgcc gtccagattt ttcgttcgtc
     1561 tgtgttgaga tggcgaagca gagcatgcag agtcgtcgtt tttcctgatc cggtggggcc
     1621 gacgcacaag atgatgccgt gcggtttctc cgcgatctcc aggagcttcg taagggtggc
     1681 tggagcaaac tccatggcat cgagcgggag cggatccttc gccgagagca gacgcatgac
     1741 gatgtcttcg ttgttgccgg cggtcggcaa tgtggctacg cgcagctcaa tttcccgatc
     1801 ctggctcatc ttgaatcgga tcttcccgtc ctgcggtttc cgtcgttcgg atatatcgag
     1861 actggacatg atcttgatac gagacaccag agctcgccga tagatcgcgg gaatcttcat
     1921 gtacgtggag cacgtaccat ccacgcgaaa acgaatggcc gtctccttgc ggtccgagta
     1981 gggttcaata tggatgtcgg aggcgtcatg ccggaatgct tcggcaatga tctgattcgc
     2041 gagccggacg atcgcggaat cgttctcgtc aatgccgtcg gaattgacgt cgagagactg
     2101 ttcctggtac gcgtcgtgga ccagctcgcc caatgtatcc gcaatagacc cacagtctgt
     2161 gtctccgatc gtgtcactca ggaaccgctc gatatcgcac cgtaggccta acgcgtaccg
     2221 gatcgtcatg ccggggaatg agcgccggat gtcatgacct ctatcgagat cgtgggggtt
     2281 atcgatcagg acatccagaa tatcaccctg ctgtttcagg gggagccaat ggtgccttcg
     2341 gagatagtct ttactgagat tattgaggag ttggggatca gccagggtcc gttcgtcgta
     2401 tggaatatag ggacagtcga aaaacgcgct cagggttttg ccgagtgcag ctttcgggat
     2461 tcggtatttc tcgagcagca ggccttccaa gtcggtcgct ccctgcaggg attcctggat
     2521 ggccttcacg aggtccgtct ggcggatgag gccctcctgg atcaaggcat cgtaggcacg
     2581 tggttcgat
//
LOCUS       DATMXI010000066         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_276079, whole genome shotgun sequence.
ACCESSION   DATMXI010000066 DATMXI010000000
VERSION     DATMXI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276079"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1413
                     /gene="bioF"
                     /locus_tag="VN444_01285"
     CDS             253..1413
                     /gene="bioF"
                     /locus_tag="VN444_01285"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWR20474.1"
                     /translation="MLEQRLQELAARHLTRHLTPLHSGTGPSVEMAGRQILLFASNDY
                     LGLAMHPEVVQSAIEATQRFGAGAGAARLISGSLPPHQELESALAQFKGTEAALTFSS
                     GYLANIGTIPALIGRGELVLADRLSHASLIDGCRLSAADFRVYRHKDTDHLKSLLAKR
                     RRGRRTLIVTDGLFSMDGDLAPLPELSQLAQDYEADLYIDDAHGTGVMGLHGRGTAEH
                     FGINADLPFQMGTLGKAFGSSGAYLAGSSTLIRYLMNTSRSFIFTTAPPPSSAAAVTA
                     ALRIMQREPERRARLWANRERLFTGLTRLGFNLSPTVTPIMPILVGDAEIALTFAQHL
                     FREGVYAPAIRPPTVPNATSRVRVTVTSEHTVNHIDQALTAFQRAGELSGLI"
     gene            1576..2634
                     /locus_tag="VN444_01290"
     CDS             1576..2634
                     /locus_tag="VN444_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWR20475.1"
                     /translation="MDISKLLTFTAKEGASDCHISAGEPPMIRINGDLKKLDHPSLTS
                     EETHALIYDMMSDTQRKNFEEKRECDFSFELGDIARFRVNVFVQHRGLGAVFRNIPTE
                     IIPLEKLGMPPIVRQLCDREKGLILVTGPTGSGKSTTLASMVDYLNNTFEGHIITIED
                     PIEFIHKTKKCLVNQREMGVHTLSFANALRASLREDPDIILVGEMRDLETIQLALTAA
                     ETGHLVFGTLHTSSAPKTIDRIIDAFPPNQQSQIRTQLAEALEAVITQTLLKRKAGGR
                     VAALEIMVATTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALFELASRGVVTKGEA
                     QSRSMNPNLFGTSMGGAA"
BASE COUNT          618 a          816 c          715 g          595 t
ORIGIN      
        1 ttgataaccc tgtgatcgat gcagcgtctg gccaagccag ctcagcatat gagcttcatc
       61 gaccggaaga gttttcgccg ggaccttgat gcggtagctc atggatggat tcgtcggaga
      121 gcgtgaggtc tgtttcctcg atttgtgtgc gcgcttatac taaggatgtc acgaatttgt
      181 caatgaggca actactacct gtcgactctc agaaggcact cgctccgcca cgtgtatgac
      241 tggcaccgtc tgatgcttga acaacgacta caagaactgg ctgcgcggca cctgacccgg
      301 cacctcacgc ccctccattc cggtaccggt ccgtcggttg agatggcagg acgccagatt
      361 cttctgttcg cctcgaacga ctatttgggg ttggctatgc acccggaagt ggttcaatcc
      421 gccatcgagg cgacacaacg gtttggtgct ggcgccggcg ccgctcgact gatctcaggc
      481 tcgttgccgc ctcatcaaga gctcgagtcc gctctggcac aattcaaggg aaccgaagcc
      541 gcgctaactt ttagctccgg ctaccttgcc aatatcggaa ctatccctgc attgatcggg
      601 cgaggcgaat tggtcctggc cgaccgtttg agccatgcca gcttgatcga cggctgccgt
      661 ttgagcgcgg ccgattttcg ggtctatcgc cacaaggata ctgatcacct caagtccctt
      721 ctggcaaaac ggcgccgggg gcgacggacc ctgattgtga ccgacggcct gttcagtatg
      781 gatggagacc ttgcgccatt gcccgaactc agtcaattgg ctcaagacta tgaagccgac
      841 ctctatatcg atgatgccca tggcactggc gtaatgggac tgcatggacg aggaacagcc
      901 gaacacttcg gcatcaatgc ggatctcccg tttcagatgg gtacgttggg taaagccttc
      961 ggcagcagcg gcgcttacct tgctgggtct tcgacattga tccgctacct catgaacacg
     1021 agccggtcct tcatctttac cactgcacct ccaccaagtt cggctgccgc cgtaacggca
     1081 gccctacgca tcatgcagcg agagccggaa cgacgtgcac gcctgtgggc taaccgtgaa
     1141 cgcctgttca caggattgac cagattggga ttcaacctgt cccccaccgt cactcccatc
     1201 atgccaatcc tcgtcggcga tgctgagatt gccctcacgt ttgcccagca cctgttcaga
     1261 gaaggtgtct atgctccggc gattcggcct cctaccgttc cgaacgcgac cagccgcgtc
     1321 cgcgtcacgg tgacttccga acacaccgtg aaccacatcg atcaggccct taccgcattt
     1381 caacgtgctg gagaattgtc gggactgatc tgatcgaatc ccctgcctca ctctgtgata
     1441 ggccaccgga gaatatactc agccgcttcc ggctattttc ttgctttttc atcatcctat
     1501 ggcatcatgc tcccacgtag tctagaagtc tgacgcgatc atttcacaat ctgcatttgc
     1561 taaggagtcc ccttcatgga tatttcgaag ctcctgacct tcacggccaa ggagggtgca
     1621 tcggattgtc acatcagtgc gggcgaaccg ccgatgatcc gcatcaacgg agatctgaag
     1681 aaactcgatc acccctcact cacctcggaa gaaactcacg cgctgatcta cgacatgatg
     1741 agcgatacgc agcggaagaa ttttgaggaa aagcgcgagt gcgatttttc ctttgaactg
     1801 ggagacatcg cgcgattccg ggtcaatgtc ttcgtccaac accgcgggct tggcgccgtc
     1861 ttccgaaata ttcccacgga gatcattccg ctggaaaaac tcggaatgcc tcccatcgtg
     1921 cggcagctct gcgatcggga aaagggactg atccttgtga ccggcccaac cggatctggt
     1981 aaatcgacca ccctggcctc catggtcgat tatttgaaca acacgttcga aggccacatc
     2041 atcacaatcg aagatccgat cgaatttatc cacaaaacga aaaaatgcct cgtgaatcag
     2101 cgggagatgg gcgtccatac cctgtcattc gccaacgcac tgagagcatc cctccgcgaa
     2161 gatccggata tcatcctcgt gggtgaaatg cgcgatttgg aaaccatcca gctcgcgttg
     2221 actgcggcgg aaacggggca cttggtcttc ggcacgctcc acacctccag cgcgccgaaa
     2281 accatcgacc gcatcatcga cgcgtttccc ccaaaccagc agtctcaaat ccgcacacag
     2341 ctcgcagaag ccttggaagc agtcattacc caaactctac tgaagagaaa agcaggcgga
     2401 cgtgtcgctg ccctcgagat catggtcgcc acgaccgcgg tccggaatct tatccgagaa
     2461 ggtaaattac accagattcc tggaatcatg caggccagtc agaaagacgg tatgcaaacg
     2521 atggacatgg cgctattcga gctggcgtcc cgcggggtcg tcaccaaagg cgaagcccag
     2581 tctcggagca tgaatcccaa tctgtttgga acctcaatgg gcggcgcagc ctaaagatac
     2641 ctgaaagaat cacgggggtt gcaatggatg ttcgcagtct gttgaaagtc atggtggatc
     2701 aggaagcgtc ggacctgtac ttgaccgtcg actcggctcc cacc
//
LOCUS       DATMXI010000067         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_210997, whole genome shotgun sequence.
ACCESSION   DATMXI010000067 DATMXI010000000
VERSION     DATMXI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210997"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1413
                     /locus_tag="VN444_01295"
     CDS             91..1413
                     /locus_tag="VN444_01295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWR20476.1"
                     /translation="MNIAIIGTGYVGLVTGVCFAEFGLHVTAVDKDEERIDRLAKGEV
                     LIYETQLGEMLRRNLEQGRIQFTTDTARAIRESLVVFIAVGTPSREDGSVDLRYVEEV
                     ARTVARNLNGYKVIATKSTVPVGTGRIIQRIIHEENEGGPRGVFDIASNPEFLREGSA
                     IEDFLRPNRVVIGADNPQAVAILQDLYRPLYLIDIPFVITTIETAELIKYASNSFLAT
                     KITFMNEMANLCEVLGVDIHAVAKAMGLDQRIGSKFLHPGPGFGGSCFPKDTWALVNI
                     ARDHGCRARVVETVIEVNEQQRLRMVQKIIGALGASSDPGSLEGITIGMLGLAFKPNT
                     DDIRQSPSLAIISELQRLGARVQAYDPAAMEQARRVLSEVDYRTDAYSAVEGADVLVL
                     ATEWNQFRNLNLEKVKGLMRRPILVDLRNVYEPERVRMLGFTYCGVGR"
     gene            1940..3106
                     /locus_tag="VN444_01300"
     CDS             1940..3106
                     /locus_tag="VN444_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWR20477.1"
                     /translation="MDILIAGVSARGLVESAVRSGLPHRIVAVDYFGDFDLGLLCTHR
                     SIKKDLNLPYDAHHLIPASSGLAWGALAYVANLENYPSVVETIAGGKPILGNSPAVLA
                     SVRDPARFFECLARAGIPAPKIAFGSKPLNLDADIRWLRKPLRSGGGHGIAVHLPGDR
                     LEADFFLQEYLDGLPCGAVFAADGRDACLLGISEQLVGRTEFGVDGFRYCGSILGPVV
                     AGQAEWGDLVGSIRQIVRAITREFHLVGVNGIDFILKGQTVYPIEINPRYTASMELVE
                     WAYGLNIFKIHLDAFQGRLPDFDLLAHPDTGCFGKAILFASGTLIFHDPQWWFGQGAR
                     DLPLAEEQIAQGKPLCTVFSRGQSRSECFDRLTRIAAEIESSCVHATTPVEEHA"
     gene            3103..>3360
                     /locus_tag="VN444_01305"
     CDS             3103..>3360
                     /locus_tag="VN444_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20478.1"
                     /translation="MIRDKQFSALSATRHVLSQGGPFRVKDGEVWVEGLSCREIVKPL
                     GTPLLVYSAMRLLDNIAEIAAAADASPWPIRIHFALKACYLG"
BASE COUNT          684 a          906 c         1014 g          756 t
ORIGIN      
        1 gtcccaatcg agcgcgccca tgggccgttg cgtgaaacgt agcccgataa ctgaatgttc
       61 gcgcagagtc attcgataag gagtaatccg atgaacatcg ctattattgg aacaggttat
      121 gtgggcctcg tcaccggggt ctgtttcgcg gagtttggtc tgcatgtgac cgccgtggac
      181 aaggatgagg agcgcatcga tcggctcgca aaaggagagg tccttattta cgagacgcag
      241 ttgggcgaga tgctgcgcag aaatctcgag caaggccgca tccagtttac aactgacaca
      301 gcgcgtgcga ttagagagtc tctggtagtc tttatcgccg ttggaacgcc ttcgagagag
      361 gatggttctg tagatctccg ctacgtcgag gaagtcgcca gaaccgtagc aaggaacctg
      421 aacggatata aagtcatcgc gaccaagagc accgtccccg tcgggaccgg gcggataatc
      481 cagagaatta tccacgaaga gaatgaaggc ggaccacggg gagtgtttga tatcgcttcc
      541 aacccggaat tcctgcgcga gggatcggcg attgaggact tcttgcgacc caaccgagtc
      601 gtcattgggg cagataatcc gcaggccgtt gccatcctcc aggatctgta tcgccccctg
      661 tatctcattg atatcccctt tgtgattacg acgatcgaga cggccgaatt gatcaagtac
      721 gcctcaaaca gtttccttgc aacaaaaatt acctttatga acgagatggc gaatctttgc
      781 gaggtcctgg gggtggatat tcacgccgta gccaaagcga tggggcttga tcagcggatt
      841 ggttcaaagt tcttacatcc gggcccgggt tttggtggtt cctgtttccc caaagatacg
      901 tgggcgcttg tcaatattgc gagagaccat ggatgccggg ctcgcgtggt tgaaaccgtc
      961 attgaggtga acgagcagca gaggcttcgg atggtgcaga agattatcgg agccctgggg
     1021 gcctcttcgg atcccggctc tctggaggga attacgatag gcatgctcgg attggccttc
     1081 aaacccaata cagatgacat caggcagtcg ccttcccttg ccattataag cgaactgcag
     1141 cgcctgggcg cccgggtgca ggcctacgat cccgccgcga tggagcaggc gcggcgtgtt
     1201 ctgtcagagg tggattaccg aacagacgcg tacagcgcgg tagaaggggc ggacgtgttg
     1261 gtcctggcca cagaatggaa tcagttccgt aatctcaatc tggagaaggt gaaggggttg
     1321 atgcgccgcc cgatcctggt agatctgcgg aatgtctatg aacccgagcg cgtgcgcatg
     1381 ttaggattta cgtattgcgg ggtcgggcga tagcccgagg agctccagag tgcccccata
     1441 gatttgccgg cttccatcag cccccgcttg cgttgggtgc ggacatgggg gcagctcggg
     1501 ctcgtcactg atgtggtggc taagactatg agcctgaacg gttctcagcg cagggtcact
     1561 acgcttccat gagattattt gtggttgacg ttcgatgatg cgttttagta cactccgcgt
     1621 ccataggtga tgcgaaagcg cgtcaggccc aagcagcttg tttaacagtc gtctccagtc
     1681 cagaatgggg agtaggccag cgccactaca gggtctttaa ccgaagcgct gcttgccgcc
     1741 acagacgtga tcatcaatat tcagcgccct accccggcgg aattactgaa gattcgggaa
     1801 ggcacgacgg tcatcagtct tctgcatccg ctgaccaacc ttgacctcgt tgcaagtgta
     1861 ccccttcctg gatgaggcgg cacccatact ccgcataacc tgctgtcgtt tgctcccaca
     1921 tcagcagaaa gaggaaccgg tggatatcct gatcgcggga gtcagcgcgc gtggcttggt
     1981 ggagtcggcg gtgaggagcg ggctacctca ccgaatcgtt gcggtcgact acttcgggga
     2041 tttcgatctg gggttgctct gtacgcatcg ctcaatcaag aaagatctga acctcccgta
     2101 tgacgcccac cacttgatcc ccgcatcttc aggtctggcg tggggcgccc ttgcctatgt
     2161 tgcgaacctg gaaaattatc catcagtggt tgagacgata gccggaggga agccgattct
     2221 tggcaatagc cccgccgtcc tggcgtcggt gagagaccct gccagattct tcgagtgtct
     2281 tgcgcgagcc ggtatccctg cgccaaagat tgcctttggt tccaagcccc tcaatctcga
     2341 tgctgatatc cgatggctgc gcaaaccgct gcgaagcggg ggtggccatg ggatcgccgt
     2401 tcacctgcct ggggatcgcc ttgaggctga ttttttcctt caggaatatc tggacggcct
     2461 gccatgcggc gcggtgtttg ctgcggatgg tcgggatgcg tgtctgctgg gtatctctga
     2521 gcagcttgtt ggcaggacag agttcggagt ggacggcttt cgctactgcg ggagcatcct
     2581 cgggcccgtg gtggcggggc aggccgaatg gggcgacctc gtgggtagca taagacagat
     2641 cgttcgcgcc attaccagag agtttcacct tgtgggcgtc aatggaatcg atttcatcct
     2701 gaagggtcag accgtctatc cgatcgagat caacccacgc tataccgcct cgatggagtt
     2761 ggtcgaatgg gcctatggtc tgaatatctt caagatacat ctcgacgcct tccaggggag
     2821 acttcctgac tttgatctcc tcgctcaccc cgatacgggc tgcttcggca aggccattct
     2881 ctttgcttcc gggaccctga tcttccatga tccccagtgg tggttcggtc agggcgctcg
     2941 agatctgccg ctcgcagagg aacagatcgc acaggggaag ccgctctgta cggtcttctc
     3001 gcgcggacaa agccggtcgg agtgcttcga tcgactcacg cgtatcgccg ccgagattga
     3061 gtcgtcgtgt gtacacgcga caacgcccgt agaagaacac gcatgatccg cgacaaacag
     3121 ttttccgcat tgtcggccac gcgccacgtc ctcagtcagg gcggcccgtt tcgtgtgaag
     3181 gacggggagg tgtgggtcga aggtctttcc tgccgggaga ttgtaaagcc gctcggcacg
     3241 ccgctcctgg tctattcagc gatgcgcttg ctcgacaaca tcgcagagat tgcagcggca
     3301 gccgatgcat ccccctggcc gatccgtatc cactttgccc tgaaggcctg ctatctgggt
//
LOCUS       DATMXI010000068         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_275744, whole genome shotgun sequence.
ACCESSION   DATMXI010000068 DATMXI010000000
VERSION     DATMXI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275744"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="VN444_01310"
     CDS             <1..1825
                     /locus_tag="VN444_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWR20479.1"
                     /translation="PYTRLYDASRGMPWTRWFLGGRLNIVHNCLDRHIRDGLGNKVAL
                     IWEGDDGGSRRCSYGELHRMVVDLAAAMRMAGVQEGDTVGLCMPISIEAVAVMFAALK
                     IGAACMQIAARSAPQEVAISLQRGNARLLFINDGYPRAGKRFDLGTTARTALKEVPTL
                     TLVVVLQRMGDAPAAEWAGRGEDEKRLIGWRTFQDSGRSAPEPATAVLDSEHTALILF
                     SSGTTGTPKTIVHTHAGALAQIVKEVGYAFDCRPDDTFYWFTNIGWMMAPWELIGVLF
                     FGGSVVLYEGTHLFPTPHRLFELIDRYGITIAGFTPTGMRGLARLGEDYGSHRLTSLR
                     ILGSTGEPLDPETWRWYFQAFGRGRCPIMNISGGTELIGCLLSPLPVMPQKEATLGGP
                     GLGMDVDVVDEDGRSIRGKPGYLVCRKPFPSMTRGFLSDPERFLQTYFPGGTDRWVHG
                     DRAQVDEDGLWYILGRTDDLIVSGGVKHDPARIEAALISFSGVPPIREAATIGVDDPL
                     KGQRIVCFVVAEPGPARYVVQEAIDAMIGHVGRVYDPKGRPDSVHFVSSLPKNLAAKI
                     PRGLIRMAYAGEGVSDISKLENPQALDEITALGQSRSGGAA"
BASE COUNT          453 a          567 c          677 g          490 t
ORIGIN      
        1 gccttacacc aggctctatg acgcctcacg tggaatgccg tggaccagat ggtttttggg
       61 tggcaggctg aatatcgtcc ataattgctt ggatcgccat atccgtgacg gcctaggtaa
      121 caaggtcgcg cttatatggg agggggatga tggggggtcg cggcgctgca gctacggtga
      181 gcttcatagg atggtggtag atcttgccgc ggccatgcga atggcagggg tacaagaggg
      241 ggataccgtg ggcctgtgca tgccgatctc gattgaagcg gttgccgtca tgttcgcagc
      301 cctcaagatc ggcgctgcat gtatgcagat cgctgcccgc tctgcgccgc aagaggtggc
      361 gatatcccta caacgtggca acgccaggct gctgttcatc aatgatggct acccgcgcgc
      421 cggcaagcgg tttgatcttg gaaccaccgc cagaaccgcg ctcaaagagg ttccaaccct
      481 cacgctggtc gtcgtactgc aacgtatggg cgatgcccct gcggcagaat gggctggtag
      541 aggtgaggat gaaaagcgac tgatcggttg gcggaccttt caggattctg gccgttcagc
      601 gcctgaacct gccacggcag tcctcgattc cgagcacaca gcccttatcc tgttcagttc
      661 cggaacgacc ggaacaccga agactattgt ccatacccat gcaggggcgc ttgcgcagat
      721 cgtcaaggag gtcgggtatg cctttgattg ccgccctgat gacaccttct attggtttac
      781 caacataggc tggatgatgg cgccatggga gctgataggc gtcctgttct tcggcggctc
      841 tgtcgtatta tacgaaggga cccatctgtt tcctactcct caccggttgt ttgagttgat
      901 cgacaggtac ggcatcacaa tagccgggtt tacacccaca ggaatgcgcg gccttgcaag
      961 gctgggcgag gactatggct ctcacaggct cacctcgttg cgcattcttg gttcaacagg
     1021 tgaacccctg gatcctgaaa cctggagatg gtattttcag gccttcggtc gaggcaggtg
     1081 cccaatcatg aatatatcgg gggggacgga gttgatcggg tgcctactgt cgcctttgcc
     1141 ggtaatgcct caaaaggaag cgacccttgg cggacccgga cttgggatgg atgttgatgt
     1201 tgtcgatgag gatggcagat cgatccgcgg caaaccaggc tatctggtgt gcagaaagcc
     1261 ctttccgtca atgacaagag gtttccttag tgatccggag aggttcctcc agacatattt
     1321 tccgggtggg accgatcggt gggttcatgg agatcgggcc caggtcgatg aggacggcct
     1381 gtggtacatc cttgggcgca ctgacgatct gatcgtcagt ggcggcgtca agcacgatcc
     1441 ggccaggatc gaggccgccc tgatctcttt ttctggtgtt cctccgatac gggaggctgc
     1501 cacaatcggc gtggatgatc ctctcaaagg ccaacgaatc gtctgttttg tcgtggctga
     1561 accaggaccg gctcgctatg tcgtccagga agccattgat gcaatgatcg gccatgtcgg
     1621 tagggtctac gacccgaagg gccgcccgga ttcggtccat tttgtcagtt cgcttcccaa
     1681 gaacctggcc gcaaagatcc cacgaggtct gatccggatg gcgtatgcag gcgaaggggt
     1741 gagcgatatc tcaaagctgg agaaccctca agcccttgac gagatcaccg cgctcggaca
     1801 gagccgatca ggaggagcag cgtgaacgat gttgccatag tatggaccgg aatgaccccg
     1861 tgtcgatcgc ggtaggttga aaacaaacct tcttggaact ggcgcagatg gccgttgccg
     1921 aggctgtgca taacgcgggc attcacatcc gcgacctgta ggccggcgtt gccggaatcc
     1981 ataacgaccg catgaaattg cctgcaagcc ttgctgaatg cgggtttctg agattgtatg
     2041 ttgcctggaa cactggtagg atatcgtgat agccaccaca cagacggtgc ggcgtatgtg
     2101 ggacgcacgg atcgttcctg gagttcgacc attgaagatg gaacgtaact actgaggtag
     2161 atagactgta ggcttttaga ctgtggg
//
LOCUS       DATMXI010000069         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_299133, whole genome shotgun sequence.
ACCESSION   DATMXI010000069 DATMXI010000000
VERSION     DATMXI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299133"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VN444_01315"
     CDS             <1..566
                     /locus_tag="VN444_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20480.1"
                     /translation="LKMVISEVDRLDRRITHLLSFSRPAPFRPMPEQLPMLIDGLLPT
                     FAGPLRDRAVRLEIDMQSDLPDVYVDPIQVEQALGEIFSNALDAMPKGGSVTIRGYRE
                     PEHGQAGSVVLEIADTGDGIAMPLLPSVCDPFFTTKADGTGLGLAIAKRYVAQNGGHL
                     EIASAPGAGTTVRITLPAVSVDRERTA"
     gene            563..1954
                     /locus_tag="VN444_01320"
     CDS             563..1954
                     /locus_tag="VN444_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR20481.1"
                     /translation="MTPSTLLIVDDERTLARSIKLFMAEQGYEAEVAENADKALDLLD
                     RLRPDLVFLDVCLPGTSGIELLRRIKAFDRNIAVIIMTAYGSIEGAVEAVKLGACDYI
                     KKPVDLDELKLLADRACESSRLRQELSYYRERDVRELPFMGIIGKCDAIREIIARIRQ
                     IAALEEPPPILIAGETGTGKGLVARTLYRQSQRASRSFIEVNCAALPATLMEAELFGY
                     ERGAFTDAKESKMGLFEAADGGFLFLDEVGDTELALQGKLLRAIEEGVIRRVGGLRDR
                     RVDVGIVAATHRDLEHEVHAARFRKDLYYRLAVITIDLPPLRERGEDILMLATHFLNR
                     FNTKYGKTVRAIDDRATRILYEYPWPGNIRELSHVIERAVLWSHGERLSVEQLPLAPS
                     PQIKPPDSLPAAGLAQWALPPEGIDLAAWERSLVEQALRESGGNQTKAATLLHLSRDT
                     LRYRLKKFGLSWP"
     gene            1990..2529
                     /locus_tag="VN444_01325"
     CDS             1990..2529
                     /locus_tag="VN444_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWR20482.1"
                     /translation="MSDKPDTQRGKKTMDTDVLVMGGLAILMVVWAFTRGKDLPLQGV
                     QAGLGLLQEVWLPLLLGFMLAGLFEVLVPRELLVTWMGKESGVQGILLGWLVGLLMPG
                     GPYIVFPLAASLLKEGMGVGPLLTFITAKSLLSPIRMFTWEVPFLGWAFVTARTVPSL
                     LLPPIVGIIGQRLFALFPR"
     gene            complement(2617..>3305)
                     /locus_tag="VN444_01330"
     CDS             complement(2617..>3305)
                     /locus_tag="VN444_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWR20483.1"
                     /translation="QTLFADLEHAQRYLPLRETIKYYLTAEYALIRRALMVLSQKLTL
                     QPEEIFYLFPAELQELLSDLKPAREKMQVRREEREIALLFAKRKWMPRVIFESSLEEI
                     GRPPMLDASQEFQAVPVSSGEAVGMVRIIDPDTLDSLSNGNGCHAHDVMVVPAVSLGM
                     FLHIRGVAGLVMETGGILAHGACLARESGVPAVILENASLLLPDRSRVRIDGNSGKVS
                     LLKCPVSPVD"
BASE COUNT          618 a         1013 c         1016 g          658 t
ORIGIN      
        1 acctgaagat ggtcatttcc gaggtggacc gactggaccg ccgcatcacc catctgctca
       61 gtttctctcg cccggcccct tttcgtccca tgccggagca gcttccgatg ctgattgacg
      121 gcctcttacc caccttcgcc gggccgctca gggatcgtgc ggtccggctt gagatcgaca
      181 tgcagagcga cctgccggat gtctatgtcg atcctattca ggtggagcaa gcgctgggcg
      241 agatcttctc caatgccctc gatgccatgc ccaagggcgg gtctgtcacg atccgggggt
      301 accgcgaacc ggaacacggc caagccggat ccgtggtcct ggaaatcgcc gacaccgggg
      361 atggaatcgc catgccactg ctcccgtcgg tctgtgatcc attcttcacg acgaaggcgg
      421 acgggaccgg ccttggcctc gccatcgcca aacggtacgt ggcacagaat ggcggacatc
      481 ttgagatcgc cagcgcgccg ggagccggca ctacggtgag gatcaccctt ccggccgtat
      541 cggttgaccg ggagcggacc gcatgactcc ctcgacgctg ttgattgtgg acgatgagcg
      601 caccctggcc cgctcgatca agctgttcat ggccgagcaa gggtatgagg cagaagtggc
      661 tgagaacgcc gataaggcgc tggatctgct ggatcgcctc cgtcccgacc tggtcttcct
      721 ggatgtctgc ctgccgggca caagcggcat cgagctgctg agaagaatta aagcattcga
      781 ccgcaatatt gccgtcatta tcatgaccgc ctacggctcc attgaagggg cggtggaggc
      841 ggttaagctt ggggcgtgcg actacatcaa gaaaccggtc gatctggatg agctgaagct
      901 gctggccgac cgggcgtgtg agagctcccg gttgcggcag gagctctcct actaccggga
      961 gcgggacgtc cgcgagcttc ccttcatggg gatcatcggc aaatgtgacg cgattcgcga
     1021 gatcatcgcc aggatccggc agatcgccgc cttggaggag cccccaccca ttctgatcgc
     1081 gggggagacg gggaccggaa aggggctggt ggcgcgtaca ctgtaccgcc aaagtcagcg
     1141 ggcgtcacgc tccttcatcg aggtcaactg cgcggcgtta cccgccacgt tgatggaggc
     1201 ggaactgttc ggatacgagc gcggggcgtt cacagacgcc aaggaatcaa agatgggcct
     1261 gttcgaggcc gctgacggag ggttcctctt cctggacgag gtgggggaca ctgagcttgc
     1321 ccttcaaggc aagctgctgc gagccatcga ggagggggtg atccgccggg tcggggggct
     1381 acgggatcga cgggtcgatg tgggtatcgt tgccgccacc caccgggacc tcgaacacga
     1441 ggttcacgcc gctcggttca ggaaggacct ctactatcgg ctcgccgtca tcacgatcga
     1501 tcttccaccg cttcgcgagc gcggcgaaga tatcctgatg ctcgccaccc acttcctgaa
     1561 taggttcaac accaagtacg gtaagacggt ccgagcgatc gacgataggg caacccgcat
     1621 cctgtacgag tatccgtggc ccggcaacat tcgggagttg agccacgtca tcgagcgggc
     1681 ggtcctgtgg agccacgggg aacgcctgtc ggtcgaacag cttcccctgg ccccttcacc
     1741 gcagatcaag ccgcctgact ccctgcctgc tgctggtctt gcccaatggg ctctcccacc
     1801 ggaggggatc gacctggctg cgtgggagcg gtcgttggtt gagcaggcat tacgggaaag
     1861 cggtgggaat cagaccaagg cggccacgtt actccacctt agtcgagaca ctctccgcta
     1921 tcggctgaag aagttcggcc tatcctggcc ctgattcgtc cttaggcctt tgaccaaagg
     1981 agggctgatt tgtcagacaa accggatacg cagaggggga aaaagacaat ggataccgac
     2041 gtccttgtca tggggggcct ggccatcctg atggtcgtat gggcctttac gagaggaaaa
     2101 gatctgccac tgcagggcgt tcaggccgga ttggggctgc tgcaggaggt ctggcttccg
     2161 ctgctgctcg gcttcatgct ggctggcctc ttcgaggtgc tcgtcccccg ggagctactc
     2221 gtcacatgga tgggcaagga gtccggcgtt cagggcatcc tgctcgggtg gctggtgggc
     2281 ctgctgatgc ccggcgggcc gtatatcgtc ttccctttgg cagcctcact cttgaaagag
     2341 ggaatggggg tcgggccact gctcaccttc attaccgcca agtcgctcct ctcgccgata
     2401 cggatgttca cgtgggaggt cccgttcctt ggctgggcct ttgtcacggc caggacggtc
     2461 cccagtctgc tcctgccccc gatcgtcggc atcatcggcc agcgcctttt cgccctgttt
     2521 ccccgataac gcccgctcct gtccaggtca agtctcttta ctgaccggag ggcagcagct
     2581 catggcgggg atccgcctac ttttgcgtaa gaccagtcaa tccacagggg agaccggaca
     2641 cttcaacagg gagaccttcc cgctgttgcc gtctatcctg acccggctcc tatcaggaag
     2701 tagcaacgag gcgttttcta atattaccgc gggcactcca ctctcacggg ccaggcacgc
     2761 gccgtgcgca agaatgcctc cggtctccat caccagtccg gccacccccc tgatgtggag
     2821 aaacatgccg agactcaccg ccggaacgac catgacgtca tgggcgtggc agccattccc
     2881 attgctcaat gagtcgagcg tatcgggatc gataatccgt accatcccca ccgcttcacc
     2941 ggaagagacg gggacggcct gaaattcctg ggaggcatca agcattggcg gcctaccgat
     3001 ctcctcaagc gagctctcga agattaccct cggcatccac ttgcgctttg caaacagtag
     3061 cgcgatctcc cgctcctcgc gtctgacctg catcttttct cgtgccggct tgagatcact
     3121 gagcagttcc tggagctccg ccggaaacag gtagaagatc tcctcaggct gaagcgtaag
     3181 cttttggctc aagaccatca gcgcccgccg aatgagtgcg tactccgcgg tcaggtaata
     3241 cttgatcgtc tcgcggagcg gcaggtatcg ttgggcatgc tcgagatccg cgaacagtgt
     3301 ctgaa
//
LOCUS       DATMXI010000070         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_299049, whole genome shotgun sequence.
ACCESSION   DATMXI010000070 DATMXI010000000
VERSION     DATMXI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299049"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1015
                     /gene="nadA"
                     /locus_tag="VN444_01335"
     CDS             53..1015
                     /gene="nadA"
                     /locus_tag="VN444_01335"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWR20484.1"
                     /translation="MKIGGLIEETEVSATLDLEGEIERLKEDLHAVVLAHYYQDSEVQ
                     DVADFVGDSLQLSQQAAKTSAEVIVFAGVHFMAETAKILNPSKQVLLPDLNAGCSLAE
                     GCPAPLFGRFRQKYPDHIVISYINCTAEIKAMSDIICTSSNAEKIINQIPKEQPILFA
                     PDQNLGRYLIKKTGRNLVLWPGTCVVHEMFSEKKLVQLMLHHPRAKVLAHPECEVGVL
                     QHADYIGSTSGILNYVKQSEDTEFIIATEPGIIHQMEKTCPNKTFIPAPPDGDCACNQ
                     CPYMRLNSLEKLYLCMKHRAPEITLDEELRLLALRPIQRMLDMS"
     gene            1035..1898
                     /gene="nadC"
                     /locus_tag="VN444_01340"
     CDS             1035..1898
                     /gene="nadC"
                     /locus_tag="VN444_01340"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWR20485.1"
                     /translation="MPLSLVQASRREALKRFLQEDIGQGDVTTLAIIPPDQQAIGHFV
                     AKAPLVLAGIDLAIETLTLLDEGLAVESRHRDGDELCEGDLAASVRGQARALLTGERV
                     ATNLLQRLCGIATLTRRFVEAVRGTQAKILDTRKTTPGLRMFEKYAVTVGGGINHRFG
                     LDDAVLIKDNHIRLAGGIRTAIETARPHKSRSHRFEVEVTTLKELQEALQYDLDAVLL
                     DNMNPDMVRQAVTCVRNHEGGSKIVVEASGGMTLDNVRAFAEAGVDWISVGALTHAAS
                     AVDMSFKIHPA"
     gene            1906..3459
                     /gene="nadB"
                     /locus_tag="VN444_01345"
     CDS             1906..3459
                     /gene="nadB"
                     /locus_tag="VN444_01345"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWR20486.1"
                     /translation="MKTDILIIGSGLAGYAAALAAAKQGAEVTLLTRSPHPEESSTFW
                     AQGGIIYQGADDSPQKLVADIVAAGAGLSSPEAASLVSREGPRLVKAILIDELGVPFD
                     ESSEDSTRWDLTAEAAHSLPRILHQKDRTGSAIQRAFIEKMASYPKVKLRCAATAVDL
                     LTISHHSVEPLDVYKPPTCIGAYVLDQASGEIFPILAKETILATGGLGRVFLHTTNPA
                     GTRGDGIAMAYRAGTRCINMQYVQFHPTTFFHPSGRFLISEAMRGEGARLVDGKGREF
                     MTDYHPDGSLAPRDVVARGIHQMMLESGEPCAYLDISHKPADQVRERFPGIYAHCLKY
                     GIDMTKESVPVVPAAHYSCGGIAVDEWGRSSLHRLRAVGEVACSGLHGANRLASTSLL
                     ECLVWGTRAGAQAAAFIARGDEYYFPQIAPWRYEREPVDPALIAQDWLSIQQTMWNYV
                     GLVRSEKRLNRAHEILRELHLEILRFYEKAEVTDAMVGLRNGIQTALAILLAAMECRQ
                     SLGCHYRID"
     gene            3721..>3907
                     /locus_tag="VN444_01350"
     CDS             3721..>3907
                     /locus_tag="VN444_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HWR20487.1"
                     /translation="MKAVRFHEYGGPEVLRYEDTPDPTIAPHEVLVKVKACALNHLDL
                     WCRKGMLGMQIPLPHISG"
BASE COUNT          874 a         1084 c         1124 g          825 t
ORIGIN      
        1 cctgtggtat actccgaata caggcgaaac attgcagttg cggagctaag agatgaaaat
       61 cggcggtctt attgaagaaa ccgaggtgag tgccacgctt gatctcgaag gagagatcga
      121 gcgactgaaa gaagatttac atgcagtggt gctagcgcat tactaccagg actctgaagt
      181 tcaggatgtc gccgatttcg tcggcgatag cctgcaactc tcccagcaag cggcaaaaac
      241 gagcgccgag gtaattgtct ttgccggcgt ccactttatg gcggaaacgg caaaaatcct
      301 aaatccctcg aagcaggtgc tgcttcccga tcttaatgcc ggttgctcac tggcggaagg
      361 gtgcccggca cctcttttcg gacgcttccg acaaaaatat ccggatcaca tcgtcatcag
      421 ctacattaat tgtacggccg agatcaaagc aatgagtgac atcatctgca catcgagcaa
      481 cgcagaaaag atcatcaacc agatcccgaa ggaacagcca attctttttg cgccagatca
      541 gaatctaggt cgttatctta tcaaaaagac cggtcgtaat ctggtactgt ggcctggtac
      601 ctgtgtggtg cacgagatgt tttccgaaaa gaagctcgtg cagcttatgc tacatcatcc
      661 tcgcgccaag gtgctggcac accccgaatg cgaggtcggt gtgcttcagc atgccgacta
      721 catcggatca acgagcggaa tcctgaatta tgtgaaacaa agcgaggata ccgaattcat
      781 catcgcaacc gagcccggca tcattcacca gatggagaag acctgcccca acaagacatt
      841 tattccagcc cctccagatg gggattgtgc ctgtaaccag tgcccttata tgcgcctcaa
      901 tagcctggag aagctctacc tgtgcatgaa acaccgtgcg ccggaaatta cgctggatga
      961 agagctgcga cttcttgcct tacggccaat ccagcgtatg ctggacatga gttaggactc
     1021 tctcctttac tcccatgccc ctctccctcg ttcaggccag tcgccgagaa gccctcaaga
     1081 gattccttca ggaagatatt ggacaaggtg atgtgacaac cctcgcgatt ataccgcccg
     1141 atcaacaggc gattggccat tttgtggcca aggcgcctct cgtgctggcg gggatcgatc
     1201 tggctatcga gaccctcacc cttttggatg aaggcttagc cgttgagagc cggcatcgtg
     1261 atggtgacga gctgtgcgaa ggcgatctgg cagcatccgt tcgtggtcag gccagggctc
     1321 tgctgactgg cgagcgtgtg gccacaaatc tgctccagcg actctgcgga attgctacgc
     1381 tgacaagacg cttcgttgag gctgtccgcg gaacgcaggc gaaaattctc gacacacgaa
     1441 aaactacgcc agggctgcgg atgtttgaga agtacgcggt gaccgtcggc ggcgggatca
     1501 accaccgctt tgggcttgat gatgcggtct tgatcaaaga taaccacatc aggctggcgg
     1561 gcggtatccg tacggctatt gaaacagcac ggccgcataa gagccgttcg caccggtttg
     1621 aggtcgaggt caccacactc aaagagttgc aggaggctct ccagtatgac cttgacgccg
     1681 ttcttcttga caacatgaac ccggacatgg ttcgtcaggc ggtgacgtgc gtgcgcaatc
     1741 acgagggcgg cagcaagatc gtggttgaag cgtcaggtgg aatgactctc gacaatgttc
     1801 gggcattcgc ggaagccggc gtcgattgga tctccgttgg agccttgacg catgccgcct
     1861 cagccgttga catgagtttc aaaattcacc ccgcgtaaac cggccatgaa aacggatatc
     1921 ctgataatcg gcagcggcct cgcaggatat gccgcggcgc tggcggcggc taaacagggc
     1981 gctgaggtca cgctgctcac gagatcgcct cacccggagg agagcagtac cttctgggcc
     2041 caagggggga tcatctatca gggggcggac gactcccctc aaaagctggt ggccgacatt
     2101 gttgccgctg gcgcaggcct gagttcaccc gaggcagcgt cgctggtcag tcgtgagggc
     2161 cctaggctgg tgaaggccat cctcatcgat gagcttgggg ttccgttcga cgaatcctcc
     2221 gaagattcaa cccgatggga tctgactgcc gaggccgcac attcgcttcc acgtatcctg
     2281 catcagaaag atcggaccgg atcggcaatc cagcgcgcgt tcatcgaaaa gatggcctca
     2341 tatccaaagg tcaagctgcg ctgcgcggcc accgcagttg atctgctcac gatttctcac
     2401 cattcggtcg aaccgcttga tgtctataaa cctccgacgt gcattggggc gtatgtactg
     2461 gaccaggcga gcggcgagat ctttcccatc ctcgcgaagg aaacgatcct ggcgacggga
     2521 gggctgggtc gcgtctttct gcataccacc aatcctgccg gcacccgcgg cgatggcatt
     2581 gcgatggcct accgggccgg cacccgttgc atcaatatgc agtacgtcca gttccacccc
     2641 acaacgttct tccatcccag cggccgattc ctcatctctg aagcaatgcg gggggaaggg
     2701 gctcgtctgg tggacggtaa gggccgggag tttatgaccg attatcatcc cgatggatca
     2761 ctggccccgc gggatgttgt cgcgcggggg attcaccaga tgatgttgga atcaggcgag
     2821 ccgtgcgcat atctcgatat ctcccacaaa ccggctgatc aggtccgcga gcggtttccg
     2881 gggatttatg cgcactgctt gaagtacgga attgacatga ccaaagaatc cgtcccagtc
     2941 gtcccggccg cacactacag ttgcggcggc attgccgttg atgagtgggg acgatcaagt
     3001 ctgcatcgtc tgcgcgccgt tggtgaggtc gcctgttcgg ggttacatgg cgccaatcgc
     3061 ctggccagta cgtccttgtt ggaatgcctg gtgtggggaa cccgggcagg cgctcaggcc
     3121 gcagcgttca tcgcgcgggg tgatgagtat tattttcccc agattgcgcc ctggcggtat
     3181 gagcgtgagc cggttgatcc ggcgctgatc gcacaggact ggctcagcat ccaacagacc
     3241 atgtggaatt acgtgggcct cgttcgcagc gaaaaacggc tgaaccgagc ccacgagatc
     3301 ctgcgagagc tccaccttga aatcttgagg ttctacgaaa aggccgaggt gactgacgca
     3361 atggtgggcc ttcgtaacgg cattcagacc gcgctggcca tcctgctggc tgctatggaa
     3421 tgccgccaaa gcctgggctg ccactatcgc atcgattagg ttgtctagag taactactta
     3481 ggctgataga ctgtagactg ttagactgta ggaacacgca cggatgagcc gggcattttc
     3541 cgaatacgac gcgacaacga tggagaccga aaaggttctg ggcgtcttga ttcggtccat
     3601 gcgtaacgac taacagcctt cagcctacag cctaaacacc tgagtagtta tgtcccggat
     3661 tccagattcc gggtatcaag agaggcaagg ccccactcta cccaagaagg aggctgctac
     3721 gtgaaggcgg tgcgattcca tgagtacggc gggcccgagg tccttcggta cgaagatact
     3781 cctgatccca ccatcgcccc ccatgaggtg ctggtcaagg tcaaggcctg tgctctaaat
     3841 cacctggatc tctggtgccg gaaagggatg ctcgggatgc agatcccgct gccccacatc
     3901 tccggtt
//
LOCUS       DATMXI010000071         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_166128, whole genome shotgun sequence.
ACCESSION   DATMXI010000071 DATMXI010000000
VERSION     DATMXI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166128"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VN444_01355"
     CDS             <1..202
                     /locus_tag="VN444_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR20488.1"
                     /translation="GPTPEINIPVGITIDAAEKALILTTLGRTNQNKTRAAEILGISL
                     KTLHNKLARYREEASSLANAQE"
     gene            209..2014
                     /locus_tag="VN444_01360"
     CDS             209..2014
                     /locus_tag="VN444_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20489.1"
                     /translation="MTLGIRGKEILGISLLVFLIVSLTLVVHLSTTTRLVVQHAAEKG
                     NLQAQQIFSGSTRVMARARGSRPGVALRSDQELRALLDASIGYSPHLVYAMITDRSDT
                     VLMHSQRSREGHKAPSRPKIEQLQEMNPLRQLLALARRHQVFETTLPLSLNDRPFGAI
                     RLGLAGSLLWNELSPAIWGSLLLALFTFPLAWAVAFGLSSLVLVPLRQIAQGVDRMAR
                     GEFDTPVAFDRHDEVGQIAEKLNLLGRQLQEDRSSLMSEKTRLEGIVHCLEDAIILLN
                     REQVVIFANPVAEALLACPLMNAVGQSLAHILGEAHLLAEAVGEVFLHQQPVRNLALT
                     VPGDRDTPLDILASAYPIMDKNGVVGGMILLKNTEPLRQIQSLVDYSRKLSDLGKLTS
                     GVAHEVKNPLNAMIIHLELLKQKLNCPTEEVARSLDVLGGEIYRLDRVVQGFLRFVRP
                     QTLDLHPLDPNKLLREVAQLTEVQGTHQGIAFSFCLDREIPPINGDHELLRQAFLNLV
                     LNACQAMPDGGTVTLTTDRTAEGRICAHVIDQGVGIPPEDLDKIFRLYYTTKPNGNGI
                     GLSLVYRIVQMHGGTIEVASKVGQGTTMTVSFPTS"
     gene            2039..2461
                     /locus_tag="VN444_01365"
     CDS             2039..2461
                     /locus_tag="VN444_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20490.1"
                     /translation="MASRTIITLLSLFLAAGCAEIKAVFTPTPSPRLPARQSPPHVPA
                     RPARQEPPPPRLSPQVSSDRETQLTEEVNAMSHGVERTLLSIDRQKLKADQWETYQTI
                     QSFLTQAREALTIKDFQQAMNLVQKAHVLSGELSKAVR"
BASE COUNT          533 a          763 c          691 g          499 t
ORIGIN      
        1 tgggccgacc ccggagatca acatcccagt cgggatcacg attgatgcgg cagaaaaggc
       61 cttgattctc acaacgctgg gacgcacgaa ccagaataag acccgggctg cggagatcct
      121 tgggatcagc ctgaagacgc tgcacaacaa gctagcccgc tatcgggaag aagcctcctc
      181 cttggctaac gcgcaggaat aagtccacat gacgcttggc atccgaggta aggaaatcct
      241 cggcatcagt cttctggtct tcctgatcgt ctcactcacg ttagttgttc acctctcgac
      301 tactacccgc ctggtggttc agcatgcggc ggaaaagggc aacctgcagg cacagcagat
      361 cttctccggt agtactcgag tgatggctcg tgcacggggg agccgtccag gggtggctct
      421 gcgcagtgac caagagctcc gcgcgcttct cgatgccagc atcggttatt ccccgcacct
      481 ggtctatgca atgatcactg accgctcgga tacagtcctc atgcacagcc agcgttcccg
      541 cgaagggcac aaggccccat cccggccgaa gatcgaacag ttgcaggaga tgaaccctct
      601 tcgtcaactc ctggcgctag cccgccggca ccaggtattt gagacgacgc tgcctcttag
      661 cctcaacgac cggcccttcg gggcgatccg actcgggctg gctggaagcc tcctgtggaa
      721 cgagctctca cccgccattt gggggagcct gctactggcg ctgttcacct tccctctagc
      781 ctgggccgtg gcctttggcc tctccagcct tgtcctggtg ccgttgcgtc agatcgccca
      841 aggcgttgac cggatggcgc gcggcgagtt cgacacgccc gtcgcgtttg atcgtcatga
      901 tgaagtcggg cagatcgccg aaaagctcaa cctcttggga cgccagctcc aggaggaccg
      961 gtcctccctg atgagcgaaa agaccaggct ggaagggatc gtgcattgcc tcgaagacgc
     1021 catcattctg ctgaacagag agcaggtggt catcttcgct aatcccgtgg ccgaggccct
     1081 gctggcctgc ccactcatga atgctgtcgg tcaatcgtta gcgcatatcc tgggggaggc
     1141 tcacctctta gccgaggcag tgggcgaagt cttcctacac caacagccgg tgagaaacct
     1201 tgcgcttacg gtaccgggcg accgcgacac accccttgat atcctggcct ccgcctatcc
     1261 aatcatggac aagaacggcg tagtcggggg gatgatcctc ttaaagaaca ccgaaccgct
     1321 ccggcagatc cagtcgctcg tggactattc tcgaaagcta tccgaccttg ggaaactcac
     1381 atcgggtgtt gcccacgaag tcaaaaaccc actgaacgcc atgatcattc atctggagct
     1441 gttaaaacag aagctcaact gccctacgga ggaggtagct cgaagcctgg acgtgctggg
     1501 aggagagatt tatcgccttg accgtgtggt gcagggcttt cttcgttttg tccgtcccca
     1561 gactcttgat cttcaccctc tggacccgaa caagctcctg cgggaggtcg cccagttgac
     1621 cgaagtacaa ggaacgcatc aggggatcgc gttcagtttt tgtctagata gggagattcc
     1681 tccgatcaac ggcgatcatg agttacttcg gcaggccttc cttaacctgg tcctcaatgc
     1741 ctgccaggcg atgccagatg gaggaacggt gacccttacg acagaccgga ccgctgaggg
     1801 caggatctgt gcccacgtga tcgatcaggg ggtaggtatc cctcctgagg acctcgacaa
     1861 gatattccgt ctctactata ctactaagcc gaacgggaac gggatcgggc tttcgttggt
     1921 ctaccggatc gttcagatgc acggcggaac catcgaggtg gcctcaaaag tggggcaagg
     1981 gaccacgatg acggtcagct ttcctacctc ttagctcgga gcgaagggtt tagcagccat
     2041 ggcctcccgg acgatcatca cgttactgag cctttttctg gcggctggct gcgccgagat
     2101 taaagcggtc ttcaccccga cgccttcgcc acgccttcca gcacgccagt cgccgccaca
     2161 cgtccccgca cgcccagcac gccaggagcc gccaccacca cgcttatccc cccaggtaag
     2221 ctctgatcgt gagacgcaac tgacggagga ggtcaatgcc atgagccatg gagtcgagcg
     2281 gactttgcta tcgatcgacc gccagaagct gaaggccgac caatgggaga cctaccaaac
     2341 catccagagt tttcttacgc aggccagaga ggcgcttacg atcaaggact tccagcaagc
     2401 catgaatttg gtccaaaagg cgcacgttct gtccggcgaa ctctccaaag cggttcgtta
     2461 acgagtaata attacccccc tcgcac
//
LOCUS       DATMXI010000072         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_275164, whole genome shotgun sequence.
ACCESSION   DATMXI010000072 DATMXI010000000
VERSION     DATMXI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275164"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VN444_01370"
     CDS             complement(<1..184)
                     /locus_tag="VN444_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20491.1"
                     /translation="MPLTVVSFKHITQILGVTDSLGLNREWVEIPLSPQSPGAVRRLT
                     NGKLEIVVDADQPFEDW"
     gene            complement(244..1275)
                     /locus_tag="VN444_01375"
     CDS             complement(244..1275)
                     /locus_tag="VN444_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HWR20492.1"
                     /translation="MIDLRSDTVTKPTDAMREAMARADVGDDVYGEDPTVNRLQDMTA
                     AMFGKKAALFVPSGTMANQLAIRLHSQPGQEVIVESRAHIVRYEQGAAGALAGVQLHW
                     VAGDRGLISAEQIEAAVRPKDPHTIQTALICLESTHNSGGGTIYPLATIERIRAVAAT
                     HGIPMHLDGARLFNAIAATTLPPASYAQHFDTLSVCLSKGLGAPVGSLLMMNDLTLLD
                     KAKRLRRMYGGSMRQSGILAAAGIYALEHHIERLKVDHDHAKRLARGLQNVPTVRINP
                     HDVDTNIVIFDVIGHRLAPPAIVAALKKEGVLINALGGNSFRAVTHLDISSAMTDEAC
                     AILSRVLAN"
BASE COUNT          441 a          583 c          581 g          496 t
ORIGIN      
        1 gccaatcctc gaagggttgg tccgcatcga cgacaatttc cagcttaccg ttagtcaatc
       61 gtctgacggc gcccgggctt tgtggggaca agggaatttc gacccattcc cggttcagtc
      121 caagagaatc agtgacccct aggatttggg tgatgtgttt gaacgataca acggtcaacg
      181 gcataggtac cccatgactg gcattctaga tttcaggggg agagctgtca atgagtcgtg
      241 atttcagttt gccagaacac gggaaaggat cgcgcaggct tcatcagtca tggcactgga
      301 gatatccaga tgcgtcacgg cacgaaaact attgccgccg agagcattga tcaagacccc
      361 ttctttcttc agcgccgcca caattgccgg gggagcaagg cggtgaccga tgacatcgaa
      421 gatcacgatg ttcgtatcga catcgtgggg gttgattctg accgtcggaa cattttgcag
      481 ccctcgtgcc aaccgcttgg cgtggtcatg gtccactttc aatcgctcga tatggtgttc
      541 cagggcatag atgccggctg cggcgaggat accggattga cgcatcgagc cgccatacat
      601 gcggcgcagc cgtttggctt tgtcgaggag cgtcagatca ttcatcatca gcagcgagcc
      661 caccggcgcc cccagtccct ttgagagaca caccgaaagc gtgtcaaaat gctgggcata
      721 ggacgccggt ggcagggtcg ttgcggcgat cgcattgaac agccgtgccc cgtccaagtg
      781 catgggaatg ccatgcgttg cagccacagc acgaatccgc tcgattgttg ccagaggata
      841 tatggtcccc ccgccgctat tgtgagtgct ctcaaggcag atcagcgctg tttgaatagt
      901 atgtggatct ttggggcgga cggctgcctc gatctgctcg gctgagatga gccctcgatc
      961 gcctgcgacc cagtggagtt ggactcctgc cagtgctcca gcggctccct gttcataccg
     1021 cacgatatgg gccctgcttt cgacgatcac ctcttggccc ggttgactat ggaggcgaat
     1081 cgcaagttga ttcgccatcg tgccagaggg gacgaagaga gcggccttct tcccaaacat
     1141 tgccgcagtc atgtcttgga gccggttgac agtgggatct tctccgtaga catcgtctcc
     1201 cacatctgcg cgtgccatgg cctctcgcat cgcgtctgtc ggtttcgtga ccgtatcgct
     1261 tcgcaggtcg atcatagtgg tcctttggga tgccgcaaga ttgtggcggc gcattctagc
     1321 aggctgttga caaagtccgc cagagttagg cgaaggttaa ggctgaggct aaggttgagc
     1381 aaacaagaac ctgtcgacac ttagccttaa cctcaacata agccttcttc actcggtgct
     1441 gctcgttttc cctcgtctgg aacagtaccc ctgtcaggcc tgactgcggc cgtcgaacga
     1501 ggttacagat aatccctcca agctcgctcg tgttctctca agggatgggc tgatggatct
     1561 tccactgcgc gcagcttttt taccggcgca tctattctat cctattcctt ctcattgtcc
     1621 cccacttcgt cttcaaaggg agtcgccgga ctgtccttca ctgcgcgcat cgagcgctat
     1681 gtatgctccc tccaagcttg ctcgttttcc ctctagggat gggggctgat tgatcttcta
     1741 ttgcgcgcgt ccaacgaggg gagtccacga ccgcgcgttg cgcgagcaca gaagatcatc
     1801 agcctccatc ccgtcttctg ctttacctct gaaaaaaccg cgtgctatag tcgccgccac
     1861 atgtttgatg acgtcaaaca gggataacat actttgcgcc atggcacata cttcgtgagg
     1921 atataatgcc tgaagttcca gctaagctct accttgataa actcttgaag gattgcagga
     1981 aaaccgcgcg agatctgaga cttctctctg gtcctgtaaa ggaaaaagcc ctgcgggcca
     2041 tggcggaccg attagcggct gacgaggaga agatcctatc ggcaaatgac aaggatgtgg
     2101 a
//
LOCUS       DATMXI010000073         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_12448, whole genome shotgun sequence.
ACCESSION   DATMXI010000073 DATMXI010000000
VERSION     DATMXI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12448"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VN444_01380"
     CDS             <1..519
                     /locus_tag="VN444_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWR20493.1"
                     /translation="GIPPPEGWSNFLGAVFSDCREGQRTPGETTGEIPMKLSHPLFKL
                     NDQKPHSLLDRRYAERVPIRYRVAYSGAEGARIVRGEGILKNLSKTGCKILSTATISL
                     GSRLTLLLYLEDAQSPVRLNDTLVSWVAKDSFSVRFPKLSPEERKRLQGVIFKNISLS
                     AVDHRRTGFRIV"
     gene            548..946
                     /locus_tag="VN444_01385"
     CDS             548..946
                     /locus_tag="VN444_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20494.1"
                     /translation="MSIQITSPVTSVAEHPDQADRRHGNRTPAVFPLFYSGMDKGQFL
                     VADGIGTNLSLRGVTIYGNRSVIPGMRIALFVSLPGMEKPLCIAQNRVSWVAGRRFGV
                     TLGPLTLKEKNHLRVFLWDRVTRLDHDHRI"
     gene            970..1353
                     /locus_tag="VN444_01390"
     CDS             970..1353
                     /locus_tag="VN444_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWR20495.1"
                     /translation="MKRVQVALGKGLQSIAESHNRRQNHRPPVEFGLMYSAQNGFGEV
                     LMGDGMVTDLSPTGLGIRGNTPVRPEMELMIFLYLPGQDPLFVMETRVAWTSGRRFGV
                     EILKMNLREQNRLRYFLRSNLAYSD"
     gene            1381..1836
                     /locus_tag="VN444_01395"
     CDS             1381..1836
                     /locus_tag="VN444_01395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWR20496.1"
                     /translation="MSGQRRNGKKMTTDTKAQTTDLRTAQRASVQVVARFSATDRFQL
                     GNGMTLDLSEIGCKLSSTERLPVGSSWNLYLTVSETSRPVLISEVQVVWAAENECGIE
                     FPDVTTRELTRLRQFIWKHLNRSTLTGGPPLFTLVEPPSPQGPHLKSVP"
     gene            1987..2652
                     /locus_tag="VN444_01400"
     CDS             1987..2652
                     /locus_tag="VN444_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWR20497.1"
                     /translation="MNRRPTQATQGNGQRASAYDRQASLIAAAASLFAAKGFNGTTTK
                     AIAKAAHVSEALVFKHFPTKRTLYAAILAEKVTVNELIEPLEDAAKKHDDHRVFTIIA
                     SYRIRPGVDSTFLRLLLFSALEGHELSEMFFGKHLKVFYDHLAAYIHTRIKDGVFRAV
                     DPLLAARAFIGVVVHHRLLHEIFGVPLHQSHEDTVAAYVDLFLTGLIKRPDDRKTVGE
                     KTR"
     gene            2649..>3175
                     /locus_tag="VN444_01405"
     CDS             2649..>3175
                     /locus_tag="VN444_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWR20498.1"
                     /translation="MNPLKQHPIVTLGVIVFFAITALVVFRLSSGAKSDARKARSITV
                     ATVAPLKQDLDIRLTYTADITPNQTVNLFSRVNGYIARLHVDKGDLVKANQLLIEIDH
                     TDYQHAVNQAKANLSAARARVAQQDASVRNATLTLNRMQALIKDQFVSQQDLDNAQVA
                     YDAAVAALDSLRAQV"
BASE COUNT          763 a          942 c          799 g          671 t
ORIGIN      
        1 ggaatccccc ctcctgaggg atggtcgaat ttcttgggtg cggtattctc agactgtcgc
       61 gaggggcaaa gaacacccgg cgagaccact ggggagatac cgatgaaact gtcccacccc
      121 ttattcaagc tcaacgatca gaaacctcat tctctcttgg accgacgcta tgccgaacga
      181 gttccaattc ggtaccgagt agcctattca ggagcagaag gggcacgaat cgtcagagga
      241 gaaggtattc tgaaaaatct ctcgaaaacc ggctgcaaaa tattgagcac agccacgatc
      301 tcgctgggta gccgcctcac cctgctccta tatctagagg atgcgcaatc ccccgtacga
      361 ctcaacgaca cgctcgtttc ctgggttgcc aaagactctt tttctgtcag gtttcccaag
      421 ctgtcaccag aagaacggaa acggctgcaa ggagtgatct ttaaaaatat cagcttgtca
      481 gccgtagacc atcggcgaac ggggtttcgc atcgtttaag catcagccca atgtcaggga
      541 ggaaaccatg tctattcaga tcacctctcc cgttacctca gttgctgaac atcccgatca
      601 agccgatcgt cgccatggca atcgtacccc cgcagtcttc ccacttttct attccggcat
      661 ggacaagggc cagtttctgg tcgccgacgg catcggcacc aatctctcac tacgcggggt
      721 taccatatac gggaatcgct cggtcatacc cggcatgaga atagccctct ttgtcagcct
      781 tcctggaatg gagaagccgt tgtgcattgc ccaaaaccga gtctcctggg ttgctggacg
      841 tcgttttgga gtgacattgg gccccctgac gttgaaggaa aagaatcatc tccgagtctt
      901 cttgtgggat cgcgtcaccc gcttagacca cgaccatcgc atctgaaact gtcacaagga
      961 ggtgccctca tgaaacgagt acaggttgca ttggggaagg ggcttcagtc catagcagaa
     1021 tcacacaatc ggaggcaaaa ccaccggcct ccggtagagt ttgggttgat gtactcggcc
     1081 caaaacggct tcggcgaggt cctcatgggc gacggcatgg tcacagatct ctcgcctacg
     1141 ggtttaggta ttcgcggcaa caccccggtc aggcctgaga tggaactcat gatctttctg
     1201 tacctcccag gacaagaccc gctcttcgtg atggaaacga gggtggcttg gacctcagga
     1261 cgtcggttcg gagtcgagat cctcaagatg aatctccggg aacaaaaccg actgcggtac
     1321 ttcctgcgat cgaaccttgc gtattctgat tagattgact tctgtcacgt ggagtacccc
     1381 atgagcggac agcgacggaa tggcaaaaaa atgacaacag acacaaaggc tcaaaccact
     1441 gatctgagga cagctcagcg ggcttctgtt caagtcgtag cccggttctc agcaacagac
     1501 cgatttcaac ttggcaatgg gatgaccctt gacctgtccg agatcggctg caaactctcc
     1561 agtacggagc ggttgcctgt agggagctcc tggaatctct acctgacagt ctcagagacg
     1621 tcccgtccgg ttctcatctc agaagttcaa gtggtctggg ctgcggaaaa cgaatgcggg
     1681 atcgaatttc ctgacgtcac gacacgagaa ctgaccaggc tacgccagtt catctggaaa
     1741 cacttgaacc gctctaccct cacaggcggg ccgcccctat tcactctggt tgagcctccg
     1801 agcccacagg ggccgcatct taagtctgtg ccctaaccca gagtatttat aggcactgcg
     1861 gctgcatcct gcctctgcat acctatacct tcccgttacc tcccccaaga gtttcaatac
     1921 cactccaatg cttgccctct aaaaatggag catgctacga tcttggtgag tgagtactta
     1981 cttaccatga atagacgacc cacgcaggcc acacagggga acgggcagcg tgcctctgca
     2041 tacgatcgtc aagccagtct cattgctgct gccgcttcac tcttcgccgc aaaagggttc
     2101 aacggtacga ccacaaaagc catcgccaaa gcggcgcacg tgagcgaggc tttggtcttc
     2161 aaacattttc cgacgaaacg gaccctctac gccgcaatcc tggcagaaaa agtgaccgtc
     2221 aacgaactca tcgaaccctt ggaagacgcc gccaagaaac acgacgatca tcgggtgttt
     2281 acgatcatcg caagctatcg cattcgcccc ggcgttgatt cgacattctt acggcttctc
     2341 ctgttcagcg ccctggaagg gcacgagctt tccgagatgt tctttggcaa acatctaaaa
     2401 gtcttctatg accacctcgc tgcgtacatc cacacgagaa ttaaagacgg cgtattcagg
     2461 gcggtcgacc cactcctggc agcccgcgca ttcatcggtg tggtcgtcca tcaccgtctc
     2521 cttcacgaga tattcggagt gcccctgcat caatcccatg aggacacagt cgcggcctac
     2581 gtcgacctgt tcctcaccgg actgatcaag aggccggacg accggaagac ggtgggagaa
     2641 aagacgcgat gaatcccctg aagcaacatc cgattgtcac actcggggtt atcgtgttct
     2701 tcgcgatcac tgcccttgtg gtatttcgcc tcagcagcgg ggccaagagc gatgcccgca
     2761 aggccagatc catcacggtc gccacagtgg caccgctgaa acaggacctg gacatacgac
     2821 tgacctacac cgccgacatc acccccaatc aaaccgtcaa tctgttttca cgggttaacg
     2881 gctacattgc cagacttcac gtcgacaaag gcgatctggt caaagcgaac cagcttctga
     2941 tcgagatcga tcacacggat tatcagcatg ccgtcaacca ggccaaagcc aacctgtctg
     3001 cagccagggc gagagtcgca cagcaggacg cgagcgtccg taacgccacc ctcacgctca
     3061 atcggatgca agcgctgatc aaagatcagt ttgtctctca acaggatctg gataacgccc
     3121 aagtcgcgta cgatgcggca gtggcggcgc tcgactcgct ccgcgcccag gtcca
//
LOCUS       DATMXI010000074         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_298507, whole genome shotgun sequence.
ACCESSION   DATMXI010000074 DATMXI010000000
VERSION     DATMXI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298507"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2162
                     /locus_tag="VN444_01410"
     CDS             <1..>2162
                     /locus_tag="VN444_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWR20499.1"
                     /translation="SSVTLAATAFSGLVLVQSVPFWLALPTAIILVVSLFHAGGVLFL
                     RRAMAQITDSPTLWNALLIGAFAILLIDLLVVSRDLLPAGIHFLVVLLGIKLLTLQQR
                     RDYRHLYVISLMAILASAALTTEAWYVPIFMLYLLTAVWTLLLYHLTQETSEIPAAVT
                     PSGMGACRATFPSRITHRVFWLTNGIVVLTVALTLAIFFLIPRIGVGVLQKTSGEAVR
                     TTGFSDRVDLGTIGSVKQDPQIVMRVELPDQSAVEKDRFYLRGLAFDQYNGQSWSHSG
                     TRQRSLNLAADGTFLVRPAGSYPLDRPSQAIRQDILLETLDTSVLFAAPFAEVVSGEF
                     LTLQADAMSGLHLPFPPSSRIRYSVVSHVPQLVANERTAASLDYPDSIRFHYLQVPTG
                     SEQVADLAHRVTQRDSTPFEQTQAILQHLLENYRYSLESDAAQLDHPLEEFLFTRKTG
                     YCEHYATAMVVMLRTVGIPARLVTGFLATEWNDYGGYFTVRQRDAHAWVEVYFPHSGW
                     ITMDPTPAVSAAVAPSGWEPLSRVAESVRLQWDRLFVHYSARDQLAVVHGVREGSDVV
                     RERMSRWASLLAAPIGQVFTRLTQMVQTFHSGMLGLITGSIVVGLSLALLMLRDRIGL
                     WAVTHFLPKSHPQVAIAQLYSRMLRILERHGVSKPPAATASEFARLVEREWKAAGPMV
                     TDVTALYHQGRFSRAPLTQGDLSRVTEQLGRLQSLART"
BASE COUNT          411 a          652 c          583 g          516 t
ORIGIN      
        1 cagttccgtc accctcgctg ctacggcgtt tagcgggcta gtgttggtac agagcgtacc
       61 cttctggctc gccctcccta cagccatcat ccttgttgtc tcgctgtttc acgcgggagg
      121 ggtgctgttc cttcgacggg cgatggcgca gatcaccgac tctccaactc tgtggaatgc
      181 tctgttgatc ggcgcgttcg ccatcttgct gatcgacctc ctcgtggtgt caagagacct
      241 tcttccggca ggtatccatt ttttggttgt gcttctgggt atcaagttac tcacgctcca
      301 acagcggcgt gactatcggc atctctatgt cattagtctc atggcaatcc tggcttccgc
      361 tgcgctgacg acagaggcgt ggtatgtccc catcttcatg ctgtatttgc ttacggccgt
      421 gtggacactc ttgctctatc acttgaccca ggaaacgagc gaaatccctg ccgccgtcac
      481 cccttcaggt atgggtgcct gtcgcgcgac attcccgagc cgtatcaccc acagagtgtt
      541 ttggttgaca aacggaatcg tcgtcctgac ggttgccctg acgcttgcca ttttctttct
      601 catcccccgt attggagtag gcgtattaca aaagacgagc ggggaagcag tgcggacgac
      661 agggttttcc gatcgggtcg atctggggac gatcggatca gtcaagcaag atcctcagat
      721 cgtgatgcgt gtcgaactac cggaccaatc agctgttgaa aaggatcgct tctatctgag
      781 aggccttgcc ttcgatcagt acaatggaca atcatggagc cacagcggaa cccgccagcg
      841 ctcgttaaat ctcgccgctg acggcacatt cctcgtccgg cctgctggca gctaccctct
      901 cgatagacca tcgcaagcca tccgtcagga tatactgctg gaaaccctcg acacctctgt
      961 gttatttgcg gcccctttcg ctgaggttgt gagcggcgag tttctcactc tgcaggccga
     1021 tgccatgagt gggctgcatt taccatttcc cccttcttcc cgtatcaggt actccgtcgt
     1081 ctctcatgtt ccgcagcttg tcgcgaacga acgaaccgcg gcgagcctgg actaccccga
     1141 ctcaattcgt ttccactacc ttcaagttcc cacggggtct gaacaggtcg cagacctggc
     1201 gcatcgcgtc acccaacggg actcgactcc cttcgagcag acgcaagcga tcctgcaaca
     1261 tctcctagag aactaccggt acagccttga aagcgacgcg gcccagctcg atcatccgct
     1321 cgaagaattt ctgttcacaa gaaaaaccgg gtactgtgaa cattatgcga cggccatggt
     1381 ggtgatgctg cgaacggtcg gcatcccggc tcgacttgtg acagggtttc tggccacgga
     1441 atggaacgac tacggaggtt acttcaccgt gcgccagcgg gatgcccatg cctgggtcga
     1501 ggtctacttt cctcactcag gctggatcac gatggacccc actcctgccg tcagcgctgc
     1561 agtcgctccc tctggttggg agcctctcag ccgagtagcc gaatctgttc ggctccaatg
     1621 ggatcgcttg ttcgtccatt atagtgcgag ggatcaactg gcagtcgttc atggagtacg
     1681 agagggtagt gacgtggtgc gtgaaagaat gagccgctgg gcttcgttgc tggctgctcc
     1741 gataggccag gtgttcaccc gcctgactca aatggtccaa acgtttcatt caggcatgtt
     1801 gggactcatc acgggatcga ttgtcgttgg cctgtccttg gccctcctca tgctccgcga
     1861 tagaataggg ctctgggccg tcacgcactt tcttcctaag tcccacccgc aagtcgcgat
     1921 cgcccagctc tatagccgca tgctccgtat cctggagaga catggcgtga gcaaacctcc
     1981 agccgcgaca gccagtgaat ttgccagact ggttgaacgg gaatggaagg cagccggtcc
     2041 tatggtgact gatgtgaccg ccctgtacca tcaggggcga ttcagccgtg ctccgctcac
     2101 ccagggcgac ctctctcgtg tcactgaaca gttggggcgt ctgcaatccc tcgcacgcac
     2161 ag
//
LOCUS       DATMXI010000075         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_147876, whole genome shotgun sequence.
ACCESSION   DATMXI010000075 DATMXI010000000
VERSION     DATMXI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147876"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VN444_01415"
     CDS             complement(<1..519)
                     /locus_tag="VN444_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWR20500.1"
                     /translation="MNEAGAGSAVIEESWHSPKGRAVREVVFGMHDGLITTIGFLSGV
                     NAANASLRVIVISGLAEAFAQTLSMGFGAYLSTKSEREFYQREIARERLEIETAPEKE
                     RDEMRQVYRSKGFPEDEVEMVVARVTANKELWLKEMMMEELGLIEKRFDDPLKVGLLI
                     GASSFVGAFLPIL"
     gene            complement(557..985)
                     /locus_tag="VN444_01420"
     CDS             complement(557..985)
                     /locus_tag="VN444_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWR20501.1"
                     /translation="MVIRHETFTVATEERLQFTDLTKRVRDLLQRYEIKQGLLTLSSL
                     HTTTALFINEWQEALLHDIQLLLDRLINEHDGYRHNDPNYSDCDRSNADSHLRSLLLG
                     RQLSIPVVDGEMSLGTFESIIFAELDGPRERQIQLQVLAE"
     gene            complement(1138..1599)
                     /gene="nuoI"
                     /locus_tag="VN444_01425"
     CDS             complement(1138..1599)
                     /gene="nuoI"
                     /locus_tag="VN444_01425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWR20502.1"
                     /translation="MIAEILKGMATTFKHIFRKPVTVSYPEERLPLAPRYRGLHMLVV
                     GEDGMERCVGCELCAVACPADAIYVEAAENTEQERHSKGERYAKVYKIHMLRCIFCGY
                     CEEACPEEAIVLGKQYELASYNRGGFVYGKDRLMMPLAEAMKQRPDLHADW"
     gene            complement(1634..2179)
                     /locus_tag="VN444_01430"
     CDS             complement(1634..2179)
                     /locus_tag="VN444_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWR20503.1"
                     /translation="MIQLTNNDTLISKGEAEEFERGLLLTTLDSLINWARKSSIWPAS
                     FGLACCAIEMMATGASRFDLARFGAEVFRGSPRQSDLMIVAGRVSRKMAPVLRRIYDQ
                     MPEPKWVIAMGACASCGGIFNTYTIVQGVDQIVPVDVYVPGCPPRPEQLIHGIMLLQE
                     KIAKERPSSHGLFHLPWRRTA"
     gene            complement(2329..2631)
                     /locus_tag="VN444_01435"
     CDS             complement(2329..2631)
                     /locus_tag="VN444_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWR20504.1"
                     /translation="MITLTESAATKVADLLKTQASSEEGLRVRVVGGGCSGLSYEMEF
                     DHPQAADQVFETNGVKVLVDPQSYTYLAGSEIDYIDSLQGAGFSLKNPNPKADCGC"
     gene            complement(2732..4351)
                     /locus_tag="VN444_01440"
     CDS             complement(2732..4351)
                     /locus_tag="VN444_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HWR20505.1"
                     /translation="MVIEGAQEKRLRTKGGQEGIARIAIISPRFEVSFWGHEYALAIV
                     GKRAITPVAALPLLAALTPKEYVVTLIDENVESINYDALADMDIIGVTGMSVQRFRMK
                     HILCELKKRGCFTVVGGAWVTVQEDYFEDLADVIFVGEAEETWPAFLHDWQVGRWKRR
                     YEQSTKSDMTRVPTPRFDLLKMHDYLYAGIQISRGCPFLCEFCDIIVTFGRLPRLKTS
                     AQVLAELDSLRVQNVKQVFIVDDNLIGNKAAIKVVLRDIVKYQQGYGYPFNFFTEATL
                     DLAEDDELMSLMLDANIEGIFIGIETPNEDSLRETKKHHNVRPAAGSLLDRVHTIQHA
                     GIEVTCGMIVGFDHDDATIFQAQQQFLSDASIPHAMLGLLYATPKTPLYDRLNKEGRL
                     DPSDISEFGTNVIPHNLSRRQLLDGYISTMQSVYEANAYFDRLEALHLRGYLGQGQLG
                     GVKYLKRHPWLRVKHEVINAAVAVVLLCRLIKSVKEKGLRREYCQRLWRALQVETLPS
                     FVSGYIVNCILHYHYHKLVQGLSSGRFVSTM"
     gene            complement(4441..5667)
                     /locus_tag="VN444_01445"
     CDS             complement(4441..5667)
                     /locus_tag="VN444_01445"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWR20506.1"
                     /translation="MALTLPIYMDHLATTPVAPEVFEAMRPYFCERFGNPASRSHSFG
                     WVAEEAVEQARVQVAHLLGCKAAEIVWTSGATEANNLAIKGVAAAYQEKGRHLITSRI
                     EHHAVLDTCKRLEREGCQVTYLPVDRTGRVDPGDVERAIRKDTVLISIMAANNEIGTL
                     QPIAEIGRIAKRHGVLFHTDAAQYVGKLPLSVDDWQVDLLSASAHKCYGPKGVGVLYV
                     RMTKPRVKLIPQMDGGGHEKGRRSGTLNVPGCVGFGAACVLAERELQTEPARLLALRE
                     RLRNDLWSRIDYLHLNGHPTDRLPGNLNVSFEFVEGESLLMALKGIAVSSGSACTSDT
                     LEPSYVLLALGMNAELAHASIRFGLGRSNTEEEVDHVSAMVRESVARLRALSPLYETA
                     REGIDVKGAQWSLRAC"
     gene            complement(5719..>5915)
                     /locus_tag="VN444_01450"
     CDS             complement(5719..>5915)
                     /locus_tag="VN444_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20507.1"
                     /translation="GKEGREPAPACIKQGGDFNNAVHIRSFKNPMLDRMVEHNAVYGD
                     CHSSIEIDNYVIVFLELSWS"
BASE COUNT         1286 a         1811 c         1511 g         1307 t
ORIGIN      
        1 gaggatcggg aggaaggccc caacgaagga ggaggcccca atcagcaggc cgaccttgag
       61 cgggtcgtcg aatcgcttct cgatcagccc caactcctcc atcatcatct ccttgagcca
      121 cagttccttg ttcgcagtca cgcgtgcgac caccatctcg acctcatctt caggaaagcc
      181 cttgctccgg tagacctggc gcatctcgtc gcgttccttc tccggcgccg tctcgatttc
      241 caaccgttcc cgagcaatct cccgctggta gaactctcgt tccgatttgg tcgagagata
      301 ggccccgaat cccatcgaaa gcgtttgggc aaaggcctcc gccaagccgg aaataacaat
      361 cacgcgtaag ctggcgtttg cagcattgac cccggacaga aaaccaatcg tcgtgatgag
      421 gccatcgtgc atcccaaaga cgacctcgcg aacggcccgt cccttcggag aatgccagct
      481 ctcctcaatg acagcactcc cggcgccagc ctcgttcatt ctctctccgc tcgcgcgggg
      541 tccgacacca acccctttat tcggccagta cctggagttg gatttgccgc tctctcggtc
      601 cgtccagctc ggcgaagata atcgactcaa aggtaccgag gctcatctcc ccatccacca
      661 ctgggatcga aagctgccgg ccaagcagga gggagcgaag gtgcgagtcg gcgttgctcc
      721 gatcacaatc agagtaattt ggatcgttgt ggcgatagcc gtcatgctca ttgatcaggc
      781 ggtccagcag gagctggatg tcatgaagga gagcctcctg ccactcattg atgaacagtg
      841 cagtcgtcgt gtgaagggaa ctgagggtga gcaacccctg tttgatctca taccgctgaa
      901 gaagatctcg cacacgcttt gtcagatccg tgaactggag tcgctcctcc gtagccaccg
      961 taaaggtttc gtgcctgatc accatgcatc cctccgttga tttgtcggac tcttcacaca
     1021 gatgtctgtc gctgaggata gcggcttccc agcctcctca agcccagaca cacaccactt
     1081 tcgattgtga acgccatgcc tccgcactgg acttaccccc cgctcgcttt gactcagcta
     1141 ccagtcagcg tggagatcag gccgctgctt catcgcctcc gctagtggca tcatcaggcg
     1201 atcctttccg tacacgaagc cacccctgtt gtaggaggca agctcgtact gtttccccag
     1261 tacaatcgcc tcctccggac aggcctcttc acagtagccg caaaagatgc agcgcaacat
     1321 gtggatcttg tacaccttcg cgtagcgctc acccttggag tgacgctctt gctcggtgtt
     1381 ctcggctgcc tcaacataga tcgcatcggc agggcaggcg acagcacaca gctcgcagcc
     1441 cacgcaccgc tccatcccgt cctcgcccac tacgagcatg tgtaggccac ggtagcgagg
     1501 cgcaagcgga agccgctctt ccggatagga gaccgtcacc ggcttccgaa agatatgctt
     1561 gaacgtcgtc gccatcccct tgagaatctc ggcaatcatc gtagccgctc ctgacgcaac
     1621 atgcgcaact tccctatgcc gtccttcgcc aagggaggtg gaagaggcca tggctgctcg
     1681 gccgttcctt ggcgatcttt tcctgcagca gcatgatccc atggatcagt tgctccggcc
     1741 tgggcgggca gccggggacg tagacgtcca ccggaacgat ctggtccaca ccctgaacga
     1801 tggtataggt attgaagatc ccaccgcaag acgcacacgc gcccatggcg atcacccact
     1861 ttggctctgg catctgatcg tagatacgtc tcaggactgg ggccatcttg cggctcacgc
     1921 ggccggccac aatcatcagg tcggactgac gtggcgaccc acgaaagacc tccgccccga
     1981 atctggcaag atcgaaacgc gacgcgcccg ttgccatcat ctcaatagca cagcaggcaa
     2041 gcccgaagct cgccggccag atcgacgact tcctcgccca attgatcagg ctgtccaggg
     2101 ttgtaagaag gaggccccgc tcaaattcct ccgcttcacc tttggagatc agggtatcgt
     2161 tattcgtcaa ctgaatcata cgatgtatcc tcgtcaccac ttaggtgttt aggttgaagg
     2221 tgatgcattg tctcgcaagg tacgaatcag atgcccgaag ttttatgtac ttactgcttc
     2281 aactctacac gccttcagcc ttcagtctct ctacctgaat tgtgacaatc agcagccgca
     2341 gtccgccttc gggttggggt tcttcagact gaagccggcc ccctggaggc tgtcgatgta
     2401 gtcgatctcg gagccggcca ggtaggtgta gctctgcggg tccaccagga ccttgacacc
     2461 gttcgtctcg aagacttgat cggccgcctg ggggtggtcg aactccatct cgtaggagag
     2521 gccggagcag ccaccgccga cgacgcggac gcgcaagcct tcctcagaac tcgcctgtgt
     2581 cttgagcaaa tcagccactt tcgttgcagc cgactcggta agggtaatca ttgggacctc
     2641 cttattattg ggagctgttg gtcgacgcct cgactcctca ctgaagagct cgtgccgttg
     2701 gctcacacga actatagcac cccttcgata cctacatcgt gctgacaaac cgcccgctac
     2761 ttaatccttg cacgagcttg tgataatggt agtgcaatat acagtttacg atgtatccgc
     2821 ttacaaaact tggcagggtt tctacctgta atgcccgcca cagtcgctgg caatactcgc
     2881 gtcgtaaacc cttctccttc acgctcttga taagccggca cagtaacacg accgcaacag
     2941 cggcatttat gacctcatgc ttcactcgca gccatggatg ccgtttcaag tacttcacgc
     3001 cgcccaactg tccctgtccc aaataccctc tcagatgcag cgcctctaaa cggtcaaagt
     3061 acgcattggc ctcatacacc gattgcatgg tactgatata gccatccagc agttgacgac
     3121 ggcttaggtt gtgaggaatc acatttgtcc caaattctga gatatcggac ggatcgagac
     3181 gaccttcctt gttcaatcgg tcgtacaggg gtgttttagg agtggcatag agcaacccga
     3241 gcatcgcgtg aggaatggag gcatctgaga ggaactgctg ttgcgcctgg aagatcgtcg
     3301 catcatcgtg atcgaatcca acaatcatac cgcaggtgac ctcaatgccg gcgtgctgaa
     3361 tggtatggac gcgatccagc aggctgcctg ccgccggcct gacattgtga tgcttcttcg
     3421 tctctcgtaa cgagtcttca tttggagtct caattccgat gaagatccct tcgatgttcg
     3481 catcgagcat caagctcata agctcgtcgt cttccgcgag atcgagtgtg gcttccgtaa
     3541 agaaattaaa tggatatccg tacccctgtt gatacttgac gatgtctctt agcacgactt
     3601 taatggcagc cttattccca atgagattgt cgtcgacgat aaacacttgt ttcacgtttt
     3661 gaacacgcaa actgtcgagt tccgccagaa cttgcgcgct cgttttcaat cgtgggaggc
     3721 gcccaaaggt gacgatgatg tcacaaaatt cgcacaggaa gggacaaccc ctggaaattt
     3781 gaatgcccgc ataaagataa tcgtgcatct tgagtaaatc aaagcgtggc gtgggaacgc
     3841 gggtcatgtc cgattttgtg ctttgttcat agcgtcgttt ccacctgccc acttgccaat
     3901 catgtaagaa tgcaggccat gtttcttccg cttctcccac aaagatgaca tccgccaaat
     3961 cttcaaagta gtcttcctgg accgtaaccc aggcgcctcc gacaaccgta aagcaacccc
     4021 gcttcttcaa ttcgcaaaga atgtgtttca tgcggaaacg ctgaacactc atcccggtca
     4081 caccaataat gtccatgtcc gctaacgcgt catagttaat ggactctacg ttttcatcaa
     4141 tcagcgtaac cacatactcc ttgggcgtca gtgctgcaag cagcggtagc gccgcgaccg
     4201 gcgtgatggc tcgtttgccg acgatggcta atgcatactc gtgcccccaa aatgacactt
     4261 caaatcgtgg gctaattatc gcaatgcgag caatgccttc ttgtccacct tttgtgcgca
     4321 ggcgtttctc ttgggccccc tcaatgacca tcaaggttgc ctttttctaa cgagaacggt
     4381 tcgctatcgt ctcgataatc cacacgcaca acgaaccgcg tgcctctcac gccatcctcc
     4441 ttagcatgcg cggagactcc actgcgcacc ttttacgtcg atcccctcac gcgccgtctc
     4501 atacaagggt gaaagcgccc gcaggcgagc cacgctctct ctcaccatgg cggagacatg
     4561 gtccacctct tcctcagtat tcgaccggcc gaggccgaaa cggatgctgg catgcgcaag
     4621 ctctgcgttc ataccgagcg ccagcaggac gtacgagggc tcaagcgtat cgctggtaca
     4681 ggcagaaccg gaggagacgg caatcccctt gagtgccatg agtagcgact ccccctcgac
     4741 aaactcaaac gacacattca ggttgccggg aaggcgatca gtcggatgac cgttgaggtg
     4801 caggtagtca atccgagacc agagatcgtt ccgtaagcgc tctcgaagcg cgagaagccg
     4861 cgcaggctcg gtctgaagct cgcgctcggc gaggacacag gccgcgccga aaccgacaca
     4921 cccggggaca ttgagtgtcc cggaacggcg gcccttctcg tggccgccgc catccatctg
     4981 cgggataagc ttgacacgag gcttcgtcat ccgcacatac agcacgccta cccctttggg
     5041 gccataacat ttatgcgccg acgccgagag aagatcgacc tgccagtcgt ctaccgacag
     5101 cggaagcttg cccacatact gagcggcgtc ggtatgaaac aggacgccat gccgcttggc
     5161 aatgcggccg atctcggcaa tgggttgcag cgtcccaatc tcgttgttcg ccgccatgat
     5221 tgaaataaga accgtgtcct tcctgatcgc tcgctctaca tcgcccggat cgacccgccc
     5281 cgttcgatcg accggaaggt aggtgacctg acagccctcc cgttccagtc gcttgcaggt
     5341 gtcgaggaca gcgtgatgct caatccgaga ggtgatgagg tgccggccct tctcctggta
     5401 ggcggcggcc acccctttta tagcgaggtt attcgcctcc gtggcgccgc tcgtccagac
     5461 gatctccgcc gctttacagc caagtaggtg cgcgacttgg acgcgggctt gttccactgc
     5521 ctcctcggcc acccagccaa aagagtggct gcgagaggcc gggttgccga accgctcgca
     5581 gaagtaagga cgcatcgcct caaaaacctc cggggcgacg ggagtcgttg ccagatgatc
     5641 catgtagatg ggaagggtga gtgccatgat gtgaccctat tcctttaccg ttttctccgg
     5701 actcagcccg caaccaactt aactccagct taattctaag aaaactatga cgtagttgtc
     5761 aatttcaatg ctggagtggc agtctccgta gacagcatta tgctccacca tcctgtccaa
     5821 cataggattt ttgaaagacc ggatgtggac agcattgttg aaatctcccc cctgcttaat
     5881 acatgcaggg gcaggctctc gcccctcttt tccaa
//
LOCUS       DATMXI010000076         3025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_43167, whole genome shotgun sequence.
ACCESSION   DATMXI010000076 DATMXI010000000
VERSION     DATMXI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43167"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VN444_01455"
     CDS             complement(<1..1462)
                     /locus_tag="VN444_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWR20508.1"
                     /translation="MSHAGHRHRHDLQAIARQAMLARGLLPDFSPEVVLEVNRLTRSA
                     TKRASAIQDLRSLLWCSIDNDDSRDLDQLTVAEPMDDGAVKILVAVADVDALVRKGTA
                     TDAHAQHNTTSVYTEAEIFPMLPLPLSTDLTSLNEDEDRLAIVIEMVVNQDGSLGRSD
                     IYRAWVVNHAKLTYHSVAAWLDGREPAPERVATVAGLDEQLRIQDRVAQTLRTVRHVH
                     GALSLETIEPRAVFENDTLTDLRVEGKNRAQELIEDLMIAANRVTARYLETRGLPSLR
                     RILRSPDRWQRIVELAAPLGAQLPPEPDAIALETFLSKRRRIDPVGFPDLSLTVVKLI
                     GRGEYVVEDSGQTSPGHFGLAVQAYMHSTAPNRRFPDLLTQRLLKAALAEHRVPYSLE
                     ELSALAQHCTEQEDHASKVERRVRKSAAALLLESKIGQRFDAIVTGASRKGTWVRIVR
                     PSAEGKVVQGFEGLDVGDRVRVELLDTNVEQGFIDFA"
     gene            complement(2122..2340)
                     /locus_tag="VN444_01460"
     CDS             complement(2122..2340)
                     /locus_tag="VN444_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20509.1"
                     /translation="MYPNSSPFRPVARSIADACRRILAVNTPIEAGGFFLGGIKGGGV
                     MGLRRLYRSIRLTVTACSSSSFGTSRYS"
     gene            complement(2385..2615)
                     /locus_tag="VN444_01465"
     CDS             complement(2385..2615)
                     /locus_tag="VN444_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002791102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HWR20510.1"
                     /translation="MFRCHVCGATESSNKLVREVFDIDGRPVLVEEIPATICAHCGEP
                     VFSRETTERVRLLVHGAAKPVKSVTMDVFAFR"
     gene            complement(2764..>3025)
                     /locus_tag="VN444_01470"
     CDS             complement(2764..>3025)
                     /locus_tag="VN444_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20511.1"
                     /translation="TQPPATRSTPTRFHGTATLDSKRVGRDAGRIADEVVAHLAGIVG
                     ANVKVTLEIEADIPSGASDHVVRTVTENCRTLKFDSQGFEQE"
BASE COUNT          610 a          910 c          891 g          614 t
ORIGIN      
        1 gcgcgaagtc gatgaaccct tgctccacat tggtgtcgag gagttcgacg cggacccgat
       61 cgccgacatc aagcccctcg aacccctgaa caaccttgcc ttctgcggag gggcgaacga
      121 tacgcaccca ggtccctttc ctggacgcgc cggtaacgat ggcgtcgaag cgctgcccga
      181 tcttcgattc aaggagcagc gcggccgccg atttccggac ccgccgctcg accttgctcg
      241 catggtcttc ctgctcggtg cagtgctgcg cgagcgcgct gagttcctca aggctgtacg
      301 gaacccggtg ctcagccagc gcggccttga gcagtcgctg cgtcagcaga tcggggaagc
      361 gccggttcgg ggccgtggag tgcatgtagg cctggaccgc caggccgaag tgaccaggag
      421 acgtctggcc ggaatcctct accacgtact cgccccgtcc gatcagcttg acgacggtca
      481 gcgagagatc gggaaaccct accgggtcga tccggcgtcg cttcgacaga aacgtctcca
      541 gggcgatagc gtccggctcg ggtggaagtt gtgcccccag gggggccgcc agctccacga
      601 ttcgctgcca tcggtccggc gaacgtaaga tccggcgcag cgacggcagg ccccgcgtct
      661 cgaggtagcg ggccgtgaca cgattcgccg cgatcatgag gtcctcgatc agctcctggg
      721 cccggttctt accctctacg cgcagatcgg tcagcgtgtc gttctcaaat acggcgcgcg
      781 gctcgatcgt ttccaggctg agcgcgccgt gcacatgtcg cacggtccgg agtgtctgag
      841 ccacccgatc ctggatgcgc aactgctcgt cgagcccggc cacggtggcc actcgctccg
      901 gagcaggctc tctgccgtcg agccaggcgg ccacgctgtg ataggtaagc ttcgcgtggt
      961 tgacgaccca tgcgcgatag atatccgagc gtccgagtga gccatcctgg ttgaccacca
     1021 tctcgatcac gatggccagc cggtcctcat cctcgttgag cgacgtaaga tcggtagaga
     1081 ggggcagcgg gagcatcggg aagatctcgg cctctgtgta gacggacgtg gtgttgtgct
     1141 gagcgtgggc atcggtggcg gttcccttac gtaccagcgc gtcgacatcc gcaaccgcta
     1201 ccaggatctt caccgctcca tcgtccatgg gctcggcaac cgtgagctga tcgaggtcgc
     1261 gggagtcgtc gttgtcaatc gagcaccaga gcaagcttcg cagatcctga atggccgatg
     1321 cacgcttggt cgctgagcgg gtcagtctgt tgacctccag aacaacctca ggtgaaaaat
     1381 ccggaagcag cccacgcgcc agcatcgcct gacgggcaat cgcttgcaga tcgtggcgat
     1441 gtctgtgtcc ggcgtgactc atctcatcgt atgtcttttt cacagactct cctatgatca
     1501 gcgattaccg gttgagtctt tcgatggcga cggcgcagat cttctgacgg cgtcggaaag
     1561 aaacgcgatc aggcgagaga gagcacggga accgaaatga actcagtagc catctcgctc
     1621 ctctctacga cctgatcgat cctctgcatg acctctgggc taaggtaaat ctctccgcat
     1681 tgctcacaga cgtcggctgg aagatcgtta atggggaaca tccctacctt ccagcgatgc
     1741 acatgctcga ttcgtctgtg gacaattggt cctctacaaa aataacaacg cgccatgttt
     1801 acggtgcctc ccttcggact cgccaattga tccagtattg tggctctccg tggtatcaac
     1861 accgctccag aaaccaacgg tctttctgat gcctgtatta tatggctttc ggcagtgccg
     1921 tcaagaaatc gggcgagcga cagtgtgcgg ctcaggagag ccactctccc ttttcgggtc
     1981 gagtcccgct ggagccacca atgatctccg tattcccctc tgccttgttt tctgaggaca
     2041 ggattgatgg cgccttcgaa gtcgcaggcg aagtgttcaa gaagacaaag ctgatcatga
     2101 aggacatttc acgaaacttc ttcaggaata tcgggaggtg ccgaacgagc ttgaagaaca
     2161 ggcggtaacc gtgagcctaa tcgaacggta gagtcgtcgc aggcccatca ccccgccgcc
     2221 tttgatgccg cctaaaaaga accccccagc ttcgataggg gtattcacgg cgagaatgcg
     2281 gcggcaggca tcggcaatgc ttcgtgccac cgggcgaaaa ggggaactgt tggggtacac
     2341 cttttctggt cctcccgcgg aacaaccaag ggaccctgtc gagtttaccg aaatgcgaat
     2401 acgtccatcg tgacggattt tacgggtttg gccgccccat gcaccaggag cctgactcgt
     2461 tcggtagtct cccggctaaa cacaggctca ccacaatgcg cacagatggt ggcaggaatc
     2521 tcttccacca aaacgggccg cccgtcgatg tcgaacactt ctcggacgag cttattcgat
     2581 gattcggtcg ctccgcacac atgacagcga aacatggtta cctccgcttg acataatcat
     2641 accactctgc aggatcaggc tcatagagcg taatgagttg tgttgtcatg gggtcgacgg
     2701 ccaggaaaac gctggtcagg cgctctattc gatggtgaag taggagaaac agagggagaa
     2761 ggactactct tgctcaaaac cctggctatc gaatttcaga gtgcggcaat tctcggtcac
     2821 agtacgcacc acgtggtccg atgcgccgga tggaatgtcc gcctcgatct ccagggtgac
     2881 tttcacattc gctcccacaa ttccggcgag atgggcaaca acctcgtccg cgatgcgccc
     2941 ggcatcccgt ccgactcgct tggagtccag agtcgcggtc ccgtggaaac gtgtcggggt
     3001 cgaccgcgtc gcgggtggct gcgtg
//
LOCUS       DATMXI010000077         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_12298, whole genome shotgun sequence.
ACCESSION   DATMXI010000077 DATMXI010000000
VERSION     DATMXI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12298"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN444_01475"
     CDS             complement(<1..414)
                     /locus_tag="VN444_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWR20512.1"
                     /translation="MQLIPIVVEQTSRGERAYDIFSRLLKDRILFIGTPIDDADSNLV
                     IAQLLFLEAEDPDKDIHIYINSPGGSVTASLAIYDTMQYIKPAIVTICMGQAASGAAL
                     LLTAGAKGKRYALPHARIMIHQPYGGAQGQASDIQI"
     gene            complement(441..1736)
                     /gene="tig"
                     /locus_tag="VN444_01480"
     CDS             complement(441..1736)
                     /gene="tig"
                     /locus_tag="VN444_01480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWR20513.1"
                     /translation="MKIDIEEISSTKRALRIELPPQSLLEKLEVAYLHLGKKARLPGF
                     RPGKIPRDVLRSRFKEQARQEAFQELVPESYSQALKEANLDPVSEPRLDEVVCEEGKP
                     FSYRATFDVKPVLQLSDYTGVEVHKEKVEATDPEVDRALEYLRERAAEYVPMDGWPAL
                     QEDLLVVDYEGFAGGKPLKGVSGQNASILLGAHQFIPEFETQLHGLKKGEVKEFPLEF
                     PTDYGRRELAGKRVLFRVTIKEVKKKQMPPLDNDFARSAGACENIHEFREKVKQDLRT
                     HKEREQVGRLKSQILEKLRAANPFDPPESLVDAEVEAILADLRRDTGGHRATPIQSEA
                     EATRTKAKELALRRVQNSLLLEAVAKQEGLVVSEEELHNEVESTAVALNQKPEAFREL
                     LQREGRIETIRMQVLERKAIDLLYERANVVEGVNLVIPA"
     gene            complement(1766..1852)
                     /locus_tag="VN444_01485"
     tRNA            complement(1766..1852)
                     /locus_tag="VN444_01485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1817..1819),aa:Leu,seq:tag)
     gene            complement(2094..>2986)
                     /gene="alr"
                     /locus_tag="VN444_01490"
     CDS             complement(2094..>2986)
                     /gene="alr"
                     /locus_tag="VN444_01490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWR20514.1"
                     /translation="PILLFGPVICAEEIDALVEHRLQPTVCSLNQARRLSEAGLGTIP
                     IHLKVDTGMSRLGVAWQEAQDLLNALKLLPNITVSSLYSHLATADAADPVTTTQQFER
                     FAKLVDALEAQGARLPLVHLANSAATLALPDTHFDLVRIGLAQYGLYPASHFQEIVVL
                     HPALSVKARIIFIKTVPPGTGVSYGHTFRTGRPTRLATVSIGYGDGIPRALSNRIDFL
                     VRGRRARQVGTITMDQCLIDVTDVPETFEGEITTLLGQDGDERILVAEWAERLGTIPY
                     EILTVLSPRLPRIICKEASE"
BASE COUNT          574 a          903 c          856 g          653 t
ORIGIN      
        1 gatctggatg tctgacgcct gcccctgggc tcccccgtag ggctggtgga tcataatccg
       61 tgcgtggggg agggcatagc gcttcccctt ggcgccggct gtcaacagga gcgcggcgcc
      121 actggccgct tgccccatgc agattgtcac gatggccggc ttgatatact gcatggtgtc
      181 atagatagcg agtgaggcag tcaccgaccc gcccggtgag ttgatataga tatggatgtc
      241 tttgtcgggg tcttcggctt ccagaaagag gagctgggcg atcaccagat ttgagtcagc
      301 gtcatcgatc ggggtaccga tgaacagtat ccggtccttc aagaggcggg agaagatgtc
      361 gtaggctcgc tcaccccggc tggtctgttc cactactatt ggaatcagtt gcattgcgtg
      421 ctactccctt tgcgtgacgc tcaggcgggg atcacaagat tgaccccttc caccacattg
      481 gctcgctcgt acagaagatc aatcgccttc cgctccagca cttgcattcg aatggtctca
      541 attcgcccct cccgctgtag caactcgcgg aaggcttccg gtttctggtt caaggcgacg
      601 gcggtcgatt cgacctcgtt gtgcaattcc tcctccgaca cgacaagccc ttcctgcttc
      661 gccaccgcct ccagcaaaag agagttttgc acccgtctta aggcaagttc tttcgccttg
      721 gtccgcgtcg cctctgcttc gctctgaata ggggtcgccc tgtgcccgcc agtgtcacgc
      781 cttagatcag cgaggatcgc ctcgacctct gcatcaacca gcgactccgg aggatcgaag
      841 gggttggcgg cacgcaactt ttcaaggatc tggctcttca ggcgccccac ctgctcccgc
      901 tccttgtgcg tccgcagatc ctgcttcacc ttctccctga actcgtggat attctcgcac
      961 gcacccgctg acctggcgaa atcattatcc agcggcggca tctgtttctt cttgacctct
     1021 ttgatggtaa ctctgaagag cacccgcttt cccgccaact cccgtcgtcc gtaatcggtc
     1081 gggaattcca gagggaactc cttgacctct cctttcttca gaccatggag ttgggtctca
     1141 aactcgggga tgaactggtg ggcgccgaga aggatcgagg cattttggcc actcacgccc
     1201 ttgagcggtt taccgccggc aaagccttcg tagtcgacca ccaggaggtc ttcctgcagg
     1261 gcgggccagc catccatcgg tacgtactct gccgctcgct cccgaaggta ctccagggca
     1321 cgatccacct ccgggtctgt cgcctcgacc ttctccttgt ggacctcaac ccccgtgtaa
     1381 tccgagagct gcagtacagg ctttacgtcg aaggtagccc ggtagctgaa aggcttcccc
     1441 tcctcgcaga ctacctcatc cagcctgggc tcgctcaccg gatccagatt ggcctccttg
     1501 agcgcctggc tatagctttc cgggacgagt tcctggaagg cctcctgcct cgcctgctcc
     1561 ttgaagcgcg aacgcaagac atctcgagga atctttccgg ggcggaatcc tggcaggcga
     1621 gcctttttac cgagatgcag atacgccacc tccagtttct ccaggaggct ttgggggggc
     1681 aattcgattc gaagcgcacg tttggtgctg ctgatctctt cgatgtctat tttcatgggc
     1741 accttggaat cctacgtcag attcatggtg cgagaggggg gacttgaacc cccatccgcc
     1801 aaaggcggac tggatcctaa gtccagcgcg tctgccattc cgccactctc gcgtgagccg
     1861 tcgagcgagg atcatggtga ctataccata cggtcaagcc cctgtccaca acactgctct
     1921 tgcgctcttg ccctaaaata gacattggga tcagtcattg cgagcgcagc gaagcaatcc
     1981 aaccgttctt cgtgccaagt ggtcgaagtt cacgagcaca gaacggtgag attgccgcgc
     2041 acccttcggg tgctcgcaat gacaggcaca tgaacgggtt gggcgatcaa tctctattct
     2101 gaggcttcct tacagataat cctcggtagg cgaggggaga ggaccgtgag gatctcgtac
     2161 gggatagtcc ccaggcgctc tgcccactcc gcgaccagga tacgctcgtc cccgtcctgc
     2221 ccaagcagcg tggtgatctc tccctcgaac gtctccggca cgtcggtgac atcgatcagg
     2281 cattggtcca tcgtgattgt tcccacctgc cgagcccgcc gaccgcgaac caggaaatcg
     2341 atcctatttg agagggcgcg ggggatacca tcgccatagc cgatagatac ggtggccagt
     2401 cgcgtcgggc gccccgttcg aaaggtgtga ccgtaactga ccccggtccc tggtggaacc
     2461 gtcttgatga agatgatcct tgctttgaca gagagggcgg gatgaaggac cacaatttcc
     2521 tgaaagtgag aggctgggta caggccatac tgagccaacc cgatccggac cagatcgaag
     2581 tgagtgtcgg gaagcgctaa ggtagcggct gaattagcca gatgcaccaa agggagcctg
     2641 gccccctgcg cctccagcgc atctaccagc ttggcaaacc gctcgaattg ctgggtggtt
     2701 gtgacgggat ctgctgcatc cgctgtggcc aaatggctgt agaggctgga gacagtgata
     2761 ttgggcaaga gtttgagggc gttgaggaga tcctgagcct cctgccaggc gactcccagg
     2821 cgggacatgc cggtatcgac cttcaggtga attgggatcg tccccagacc tgcctcggac
     2881 aagcgacggg cctggttgag gctgcagaca gtcggttgca gccgatgctc gacaagcgcg
     2941 tcgatctcct ctgcgcagat cacggggccg aagaggagaa tcggcg
//
LOCUS       DATMXI010000078         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_130631, whole genome shotgun sequence.
ACCESSION   DATMXI010000078 DATMXI010000000
VERSION     DATMXI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130631"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1609)
                     /locus_tag="VN444_01495"
     CDS             complement(431..1609)
                     /locus_tag="VN444_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR20515.1"
                     /translation="MRANFSPDQKRTIATLSVTIAIRMLGIFLVLPVFTLYGEQLTSS
                     KPLIGLAFGSYGLTNALLQIPFGRLSDRFGRKPLLLIGLVLHSVGSILAAVPPNIFAL
                     IAARLIQGTGAVSSAAFALVADSVDEKNRATAMAFLGATIGLSFIGGILAGPMIASFS
                     GYASLFWLSGLLSLLAAGYLALAVKEPPRERTTTDISSDRLSVVSVLKIPDIVKLDIC
                     GFLTSFFISSFFFYFPLLARRHLPLHNYYLLLGPMLLVGVAVMFGASRAADRGWAKSA
                     GVTAFVVLSISAWLLFREASLGPHAHPLMVLMIAGMFFFAGFSSLEPILPSLITKASP
                     KTVYGTALGTYHSLQFLGSFAGGVTAGFLSTLGMEFLLVALLVAAASGIILMATVKTA
                     "
     gene            1726..2451
                     /locus_tag="VN444_01500"
     CDS             1726..2451
                     /locus_tag="VN444_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWR20516.1"
                     /translation="MNIEVAFSRLDPACRSASGRAVAVIDVIRATTTITMALHQGCAG
                     VIPVRTLSEARTVARRLDKGALLAGERGAERAAGFDLGNSPAEYGRERVKGKTVVLTT
                     TNGTRTFHAVAGAQVIIACSFLNVSAAAHWLIATGFDILIVCAGRQGRFCLEDCVGSG
                     MLIDRVLNISDDSIECSDAAGAAHTLFTAYRDDPLRMLRGCEWGREIIHKGFGSDLEI
                     CAQVDLTDIVPVMRKGCLVAERA"
     gene            complement(2597..>3038)
                     /locus_tag="VN444_01505"
     CDS             complement(2597..>3038)
                     /locus_tag="VN444_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20517.1"
                     /translation="FFTFPNSVVFEGVEELGDEPQQKLGETGGQTPFMHFQLAATGID
                     HDDDPYFPRMRQYMSQPCWELVEFVKQTSNIRSFILERRQNKALVQSYNALIQAIPLD
                     WRIVHMSLVDDFIAQFGEGHGTGKPPLDWLRTLHKKAASYLIEL"
BASE COUNT          676 a          795 c          956 g          611 t
ORIGIN      
        1 atggaggggg ttttcatgat gcatgggtgg ccccccggcc aagactgtta gtaaaataac
       61 gaacttgaga agattcaact caatagagcc tcgctagaac ctcttcgggc acctgggctc
      121 gcaggcccgg gtacacctcc aggagccgcg caatatcttg gccagatcct tctgccgctt
      181 gctgggccgt ctggtcggat cgagaactgc ccggatttac cctggaggac gtcttccact
      241 ctcgccacca gtagcatcat gccaaggaca ttgcgtgccg tcgcagcggg caggaacggg
      301 aaataacgag gacatgagaa ggttcatgtg cgcttgacct ttgtgaggcc ggtcggtaca
      361 ctgccgatgg tgtagacggt gtagttcgtg acggagaagg cgaaatagcg aaagagatcg
      421 tactgcccga ttatgcggtt tttaccgtgg ccatcaatat aatgcctgac gctgcggcaa
      481 ccagcagcgc gactagcagg aattccatcc ccagagtgct gagaaatccc gcagtgactc
      541 cgcccgcgaa actgccgagg aattgcagag aatggtacgt ccctagtgcc gttccataca
      601 cggtcttagg tgatgctttg gtaatgaggc tggggaggat cggttcgaga ctcgagaagc
      661 cggcaaagaa aaacatgcct gcaatcatga gtaccatgag cggatgagcg tgagggccaa
      721 ggctggcttc tcgaaagagt agccatgcgc tgattgacag gacgacgaag gccgtcacgc
      781 ctgctgattt tgcccagccc ctgtcggctg ctcgggaggc cccgaacatc acggcaacac
      841 cgacgaggag catcgggcca agcaagaggt agtagttgtg caggggcagg tggcggcgag
      901 ccagtaaggg aaagtagaag aagaagctgc tgatgaagaa cgacgtcagg aatccacaga
      961 tatccagctt gacgatatcg gggatcttga gcaccgagac gaccgagagc cggtcgctgg
     1021 agatgtcggt cgttgttcgc tcccgtggcg gctccttgac ggcaagcgcc aggtagccgg
     1081 ctgcgagcag actcagaagg ccggacagcc agaagagaga cgcgtaaccg ctaaaactcg
     1141 cgatcatcgg cccggccagg atgccgccga tgaaggaaag gccgatggtg gccccgagga
     1201 aggccattgc ggttgcgcgg ttcttctcgt cgaccgaatc ggcgaccaga gcgaatgcgg
     1261 cagagctgac ggcgccggtc ccctggatca ggcgggcggc gataagagcg aagatgttgg
     1321 gaggcacggc ggccagaatg gagcctacac tgtgaagcac aaggccgata agcaagagcg
     1381 gtttccgccc aaatcgatcc gacagccggc caaatgggat ctgaagcagg gcattggtca
     1441 gtccgtagct accgaaggca aggccgatca gcggttttga gctggtcaat tgctctccat
     1501 agagcgtaaa gacgggcagc acgagaaaga tgccgagcat ccgaatcgcg atggtgacgc
     1561 tcaacgtggc gatcgtgcgc ttttgatccg gcgaaaaatt tgcacgcatt gtgtggaagc
     1621 tttctgctaa agggagatgg ccgtctgttc atagcaggtc tacagagggg tgtcaataat
     1681 atcagcgcag gatgccggca tggttactga tctgggatca ccattgtgaa catcgaggtg
     1741 gcattcagtc ggttggatcc agcctgccgg tctgcctccg gtcgagcggt agccgtcatc
     1801 gacgtgatac gggcgacgac gaccatcacg atggcgctgc accagggttg cgccggtgtc
     1861 attcctgtgc ggacgctcag cgaggccagg accgtggcgc ggaggcttga caaaggggcc
     1921 ttgcttgcag gagaacgggg cgcagaaagg gcggcaggct ttgacctcgg gaattctccg
     1981 gctgagtacg ggcgagagcg cgtcaagggg aagacggttg ttctgacgac gacaaacggg
     2041 actcgtacct tccatgctgt tgcaggggct caggtaatca tcgcttgctc attcctgaat
     2101 gtatccgctg ccgcacattg gcttatagct accggctttg acatcctcat tgtatgcgcg
     2161 ggccgacagg gccgtttctg cttggaagat tgtgtgggca gcgggatgtt gatcgatcgc
     2221 gtactcaaca tctctgatga ttcgattgag tgcagtgatg ccgccggggc cgcccacaca
     2281 ctctttacag cctatcgaga cgacccgctt cggatgcttc gaggctgcga atggggaaga
     2341 gagatcatcc acaaaggctt tgggtcggac ctggagatct gcgcgcaagt cgatctgacc
     2401 gacatcgtac ccgtcatgcg caaaggttgc ctcgtggccg agcgcgcatg agcggcgata
     2461 gtcggccagc taatcgttcg ctgccgtagt cttttgcgtt gcactcctat tcgtcggcct
     2521 tcggtgtaca gtacagtatt ctaacgggtc aaggataccg atacgatgaa actgatcttg
     2581 gagggcatgg gaatccttac agctcgatga ggtatgacgc ggcttttttg tgcagcgtac
     2641 gcagccagtc aagcggcggc ttccctgtac cgtgcccctc gccgaactgc gcgatgaagt
     2701 catctacaag actcatgtgc acaattcgcc agtcaagcgg gatggcctga atcagggcgt
     2761 tgtacgactg cacaagcgcc ttattttgcc gtcgctcaag aataaatgat cggatgttcg
     2821 aggtctgctt gacgaattcg acgagttccc agcagggctg tgacatgtac tgccgcatac
     2881 gcggaaagta cgggtcgtcg tcatggtcga tgccggtcgc cgcaagctgg aaatgcatga
     2941 aaggggtttg tccccctgtt tcccctaact tttgttgagg ctcgtccccc agttcctcca
     3001 cgccctcgaa caccacgctg ttggggaagg tgaagaac
//
LOCUS       DATMXI010000079         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_187370, whole genome shotgun sequence.
ACCESSION   DATMXI010000079 DATMXI010000000
VERSION     DATMXI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187370"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..458
                     /locus_tag="VN444_01510"
     CDS             240..458
                     /locus_tag="VN444_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR20518.1"
                     /translation="MNKHLLRVDEAAMLLSVSRWTVYRWVQEGRLEATKVGKGSLRIF
                     QTSVATLIEQNRTWGAKPSKVPQSPTRC"
     gene            497..1507
                     /locus_tag="VN444_01515"
     CDS             497..1507
                     /locus_tag="VN444_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWR20519.1"
                     /translation="MIDLLTPLSVIVLWLDFAGLTTLIGTLAFQYLIIRPVSLSPRGF
                     EPFEGLLRRVEAGALMLVALVSVLELLLRALVMGGGILSNAAGALPSMLLDTRQGTIW
                     VVRIALVGILGLGWLFGPQRTWFTGIRLLGAVAIALTRSLSGHAADWGDVTLPVLIDW
                     LHLLAVSIWIGGLFVMGFVLRAALTPTSNEDAAMGFALIARRFSILATVCVVGLLTTG
                     LFHPRLRLMSFSTLFWIPYGWTLMVKLSLVLLMLMLAALNRSYLLPRLGRGSDGRARL
                     LVTTIGRLEWLLAVFVLVSSAWLTQLTPARHISRYEYREPHAVHRQAGDVAPAPSPTE
                     RQ"
     gene            1683..2054
                     /locus_tag="VN444_01520"
     CDS             1683..2054
                     /locus_tag="VN444_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWR20520.1"
                     /translation="MTPRKGLVLFVAMAAVLFLGSFAPPIEAGQVTLHLVDVDAQGVQ
                     IWTPTTIIAKKGDTVTLMLNNSLREEHGYEIEGVGVKEVVGANQTKTVSFKATKAGIF
                     PIKCQLHKATLHMAGQLLVLE"
     gene            2841..>3030
                     /locus_tag="VN444_01525"
     CDS             2841..>3030
                     /locus_tag="VN444_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20521.1"
                     /translation="MGTTGGVIVYNAHIERPARTTSRRISNSKRAHGSRDYFSEALRI
                     LDNDRTLEPQREHLAALWG"
BASE COUNT          591 a          922 c          842 g          675 t
ORIGIN      
        1 ccatcgcttg tctcgatccc gcccacggac gcgctcctcc ttctttcact gcctgaactt
       61 cacgctattg ctcagaaaat ggcatccggc agtcagcact cagccgtagg tcgggttagc
      121 gcagcgtaac ccgacaactc ggccaaggcg aaggaagtat cagaggctga acgctgaccg
      181 ctgaaagctg atcacttagg cagaattgca ttgacgagtc attagaagga gtaacagcca
      241 tgaacaagca tttattgcgc gtcgatgaag cggcgatgct gctgagcgtc agccgttgga
      301 ccgtgtatcg ttgggtgcag gagggtcgat tggaggcgac caaggtcggg aaagggagtc
      361 tccgcatctt tcagacctct gtggctacgc tgatagagca gaaccggact tggggcgcca
      421 aacccagcaa ggtcccccag agcccgacgc ggtgctaggc ccttgacggg ccagagatga
      481 accgttcgtg acaaccatga tcgaccttct aacgcccctc tccgtcatcg tcctctggct
      541 cgacttcgca gggctcacga cgctgatcgg cactcttgcg tttcaatacc tgatcatccg
      601 accagtctct ctatcccctc gggggttcga gccctttgag gggctgctcc ggcgggtaga
      661 ggccggggcg ctcatgttgg tggccctggt gagcgtacta gagctgctcc tcagggcgct
      721 ggtcatgggc ggcgggatcc tgagcaacgc ggctggcgcc cttccgtcga tgcttctcga
      781 tactcgccaa ggcaccatct gggtcgtacg aatcgccctt gttggaatac ttggcctcgg
      841 ctggctgttt ggcccccagc gcacctggtt cactggtatc cgtcttctcg gggctgtcgc
      901 tatcgccctc acccgaagcc tatcgggtca tgcagcagac tggggagatg tgacactccc
      961 agtcctgatc gactggctcc atctgctcgc agtctccatc tggattgggg ggctgtttgt
     1021 gatgggattt gtgctgcgcg ccgcgttgac accgaccagt aacgaggacg ccgccatggg
     1081 cttcgccttg atcgccagac gattctcaat cctagcgacc gtctgcgtcg tcggccttct
     1141 cacaacggga ctcttccacc cacggttgcg actgatgtct ttctcgaccc tcttctggat
     1201 tccctacgga tggaccctca tggtgaagct ctcgcttgtc ttgctaatgc tgatgctcgc
     1261 agcgctgaac cgctcctacc tcctcccccg actaggccgg ggttcggatg gccgagcccg
     1321 attgctcgtc acgaccatag gtcggctaga gtggctccta gctgtgttcg tgctggtctc
     1381 cagtgcatgg cttactcaac tcacgcccgc ccgccatatt tctcgttacg aataccgtga
     1441 accgcatgct gtccaccggc aggcagggga cgtagcccca gcaccgtcgc ccacagaaag
     1501 acagtgatac gcttcgcgac atggtatgta aaacgactga gtgaaataat agtgtcacag
     1561 gcatcggcaa ccgtgagcac cattgttaga cgagaagctg tgacagggta cacacttgag
     1621 tatgaggttt tttgagcgct ccacggttgt ctaaccaggc tgattgaaga aggagaagaa
     1681 acatgacgcc cagaaaggga ttggtactct tcgttgcaat ggcggccgta ctcttccttg
     1741 gcagcttcgc tccgcccatc gaagccggac aggtcaccct gcacctggtg gacgtcgacg
     1801 cccaaggtgt ccagatatgg acgcccacca cgatcattgc caagaaggga gacacggtga
     1861 cgctcatgct caacaacagc ctgcgggagg agcacggcta cgagatcgag ggtgttggtg
     1921 tgaaggaggt ggtcggtgcc aatcagacca agaccgtatc cttcaaggcc actaaggcag
     1981 ggatcttccc cattaagtgt cagcttcaca aggccacttt gcatatggcc ggccaactgt
     2041 tggtcctcga gtagcagagg cggctccggc tcctttggcc gagcggaact ccggccttga
     2101 gggattgcgg taatccctta aggccggttc ttattttgcc gggattccgg catagccgcc
     2161 cacctccgat cgcggcgtgg gcgttcagca ttttgtagca gcactgagca ggcatctgta
     2221 gacacgctcg tccgaagaaa gtataaagcc ctgtagaact gtgttctccc tctgtgtcgt
     2281 ctgctaaagg agacggagtt ttgcccgttg cgctcttgaa gagaccatcc cacaaagctc
     2341 agcctctgag gcccctggcg gtctcggcgc ttggcctcta tctactggtt cacgtattcc
     2401 ttgtcctgtg tctggtccat cctcacaatg cccacctcca agaccaggca gatagtcatc
     2461 ccctctctgt ctgcgcctgg gttcataaga cagtgtcctc gcacgtcccc tcgtcaggga
     2521 tcgtcctgcc cctggttgcg gctgcgctgc tcgtctacct tctattgcct cagcattgtc
     2581 ctcaaatctc ccccatcaga ctgaccggtc gttctccacc cctactcgtc ttctcgtaac
     2641 tctttccatc cgcgtactcg acccatacac actacgatta ttcgcttgaa ccaagacgcc
     2701 taagcctatt ccaggacttg ggcaggttct gagaaccagt ggttccgttg tagccgccta
     2761 taggctctac ggccactggc gcgcgtcggc atcgcaatat cgtgttgctc gctgtcctcc
     2821 gacataggga ggccgtcgac gtgggcacaa caggaggtgt catcgtgtac aacgcgcaca
     2881 ttgaacgacc agctcgaact acgagtcgac gcatatccaa cagcaagagg gcccacgggt
     2941 ctcgtgacta cttcagtgaa gccctccgga tcctggacaa cgacagaacg cttgaaccgc
     3001 agcgggagca cttggcggcg ctgtgggggc
//
LOCUS       DATMXI010000080         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_42834, whole genome shotgun sequence.
ACCESSION   DATMXI010000080 DATMXI010000000
VERSION     DATMXI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42834"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..519
                     /locus_tag="VN444_01530"
                     /pseudo
     CDS             412..519
                     /locus_tag="VN444_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
     gene            699..1883
                     /locus_tag="VN444_01535"
     CDS             699..1883
                     /locus_tag="VN444_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20522.1"
                     /translation="MMIVRRITALVQQRLKSYPAVALIGPRQSGKTTLARSLATDYFD
                     LEQEPERLRLDVQWPSLIKTKRLVVLDEAQTWPELFPRLRGAIDEERKRHNRFLLLGS
                     VAPSLMKHVSESLAGRLSLVELSPFSLNELADTPLTELWLRGGFPDGGILTPARYPQW
                     QKDYLDLLTQRDLPNWGLSAKPHVMQRLLKMLAAVHGQLWNASQIGQSLGLAYHTVNN
                     YVHFLEGAFLIRKLHPWSANLRKRLVRSPKCYWRDTGILHSLLGVQDYDALLNQPWVG
                     ASWEGFVIEQILDTLNATGRPVDPWFFRTADGSEIDLLFQWDHSLWAIEVKLTSHPSL
                     QDFDSLNRAADLVGADKRVLVSQTTQSAIGKDQISCSLPELLKIFVQPTLYRKRRLRG
                     MS"
     gene            complement(2171..>2677)
                     /locus_tag="VN444_01540"
     CDS             complement(2171..>2677)
                     /locus_tag="VN444_01540"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HWR20523.1"
                     /translation="VLKANGVAHRAWLAEGHGDPREQFAHALIAACAGVNTVLAYNAP
                     FECRCIDGLIEVLPHMEGELVALSRRIRDLLPIVRDHVYHPDFGGSFSIKTVLPALVP
                     GLGYDDLEIHDGGTAAAALETLLLGADTLTAEQQRSVRRELLRYCERDTLGMVRLYER
                     LRALAGLR"
BASE COUNT          552 a          763 c          786 g          576 t
ORIGIN      
        1 cgtcctgggt cagcgtctga tcgatccgga tcgcgacttc tttacccggg atcagccccc
       61 cggcgacccg gtgcgcctca aaaatcttct gaaccaggtt cattcccatc gcttcaccct
      121 tccaacgatc cttgatgcag ccttattcga gatctggaat tgcattaaac gtgctgctcc
      181 acagccgttt gagcctacca aaagcatgct actggaagct cctcgcatcg tcaacggaaa
      241 aggcccagcg ggtagtgaga tgacaagctg caccgaggcg cggtatgatc tgatgtagtg
      301 cagggtacgg tctttggacc gtgatgggga tgggatgccg cgcgaagggc tctgccgccg
      361 aggagagcag tgaggcccat gcaggcttat ggcgatcgaa caggggagca gatgaggtgg
      421 atgctccata ccgacatgga cgccttctat gcctctgtgg agcagcgcga ttacccggcc
      481 tctcgcggac gccccgtgat cgtgggggcc gatccccacg gcggcagagg ccgcgggatc
      541 gcccggggta gagcgcgagc gcgttatgga gcgaggccga aatactactc acgccccagt
      601 accgaccctt gattttccac tgcgcaccgg cgggtacccc tcgcaaccat tgtagaaata
      661 ccttttaatt gcaagataga tattctattc tattttgcat gatgatcgta agaagaatca
      721 cagcgctggt acaacagcgc ctgaaatcgt acccggcagt ggcgttaatc ggacctcgtc
      781 agtccggcaa aaccacgctg gcccgctctt tggcgactga ttatttcgat ctggaacaag
      841 aaccggaacg actgcgtctg gacgtgcaat ggccgtcgtt gatcaagacc aaacgtctcg
      901 tcgtgctcga tgaagcccag acatggccgg aattatttcc acgtttgcgt ggggcaattg
      961 acgaggaacg caagcggcat aaccgcttcc tgctgttggg ctccgtcgcc ccatcgctca
     1021 tgaagcacgt gtctgaatcc ctggcggggc gtctttcgct ggttgagctc agtcccttta
     1081 gtctcaatga gcttgctgac accccactga cggaactgtg gttgagaggc ggcttccctg
     1141 atggcggaat attgacgccc gcccgttacc cccaatggca aaaggactat cttgacctcc
     1201 tgacgcagcg cgatttgcca aactggggac tgagcgccaa accacacgtg atgcagcgcc
     1261 tgctgaagat gttagcagcc gttcatggcc agttgtggaa tgcctcgcag attggccaaa
     1321 gcctcggtct tgcgtaccac acggtcaaca actatgtcca ttttctggaa ggggcttttc
     1381 tcattcgcaa gcttcatcca tggtcggcca atctgaggaa gcggctcgtc cgaagcccca
     1441 agtgttactg gcgtgatacg ggcattctcc acagtttgct cggcgttcag gattatgacg
     1501 cgctcttgaa ccaaccgtgg gtaggagcca gttgggaagg ttttgttatt gagcagatat
     1561 tggacacgtt gaatgccacc ggacggccgg tggatccatg gtttttccgt acggctgacg
     1621 gctccgagat cgatctcttg ttccagtggg atcatagctt gtgggccatt gaagtgaagc
     1681 tgacatcgca tccttcccta caagattttg acagcctcaa ccgtgcggca gatctggttg
     1741 gagcggacaa gcgcgtactg gtctcccaga caactcaatc ggcgataggc aaggatcaga
     1801 tctcatgctc gttaccagag ttgttgaaga tattcgttca acccacgctg tatcgtaagc
     1861 gtaggttgcg ggggatgtca tgaaccccaa ccaatgcggc gccatagcca tagtttccca
     1921 cgaatggctt tcgggtaggg ggtgacggtc ttgaaaatag ggacagacac tataggaaaa
     1981 tagggacaga cactattatt gtggcgttta cggtcatgat cgtgcatgcc acggattact
     2041 cgaatctaca catgcagttg cgtgagcttc tcttcgcgtt gggcatcgtc tggttcgctc
     2101 ggtggatcca tgcaggcgat cacgcaccga aggtgccccg ggttggctag ctgggacaag
     2161 atcgaagcac tcatcgaagc cccgcaagtg cgcgcagcct ctcgtacagc cgcaccatac
     2221 cgagggtatc gcgctcgcaa tagcgaagca gctcgcgccg tacgctccgc tgttgctccg
     2281 cggtgagggt gtcggcgccg agcagcagtg tctcgagtgc cgccgccgcc gtgccgccgt
     2341 catggatctc gaggtcgtcg tagccgagcc cgggtacgag cgccggcagt accgtcttga
     2401 tgctgaaact tcccccgaag tccgggtggt agacgtggtc gcgcacaatc ggaagaagat
     2461 cgcggatccg gcgcgagagc gccaccagtt cgccctccat gtgcgggagc acttcgatca
     2521 gtccgtcgat gcaccggcac tcgaatggcg cgttgtaggc gagcaccgtg ttgacgcccg
     2581 cgcacgccgc gatgagcgcg tgcgcgaact gctcacgcgg gtcgccgtgg ccctcagcca
     2641 accatgcgcg gtgtgcgact ccgttggcct tgagtac
//
LOCUS       DATMXI010000081         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_99755, whole genome shotgun sequence.
ACCESSION   DATMXI010000081 DATMXI010000000
VERSION     DATMXI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99755"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VN444_01545"
     CDS             complement(<1..518)
                     /locus_tag="VN444_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HWR20524.1"
                     /translation="MNNAQSGILESTPPVARYLIFSLTDHGDARRSLRALRDIADGRQ
                     TVVGLGQSLVLALGATVPGLRTFPSYAGGGFDVPSTPFALWCWLRGDDRGELLHRARR
                     INHALAPAFRLDHVIDAFTYGTGRDLTGYEDGTENPKDERALEVAFVRGQGDGLDGSS
                     FVAVQQWVHDLDR"
     gene            complement(550..1308)
                     /locus_tag="VN444_01550"
     CDS             complement(550..1308)
                     /locus_tag="VN444_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20525.1"
                     /translation="MASSVLIGAWVAAGLTLFMFSFLYKDNPFFRFGEHLYVGVSMGY
                     TIVRIYYDVMVKSLYVPVTQEGKWWFLIAALLGLLILTRFIPKVSWLSRISFAVIVGF
                     GSGVAIPRIISSNILQQVQGTLKPILNSGQSPFGMAQFNALLILVGVITVLIYFFFSI
                     EHKGPIKVAARIGIYFLMINFGAGFGYTVMARMSLLIGRFDDLILFASRDYGYASLIL
                     LGLIAFGLFLWERRSSALSDPDQDRRTAGSDPHA"
     gene            complement(1308..>2290)
                     /locus_tag="VN444_01555"
     CDS             complement(1308..>2290)
                     /locus_tag="VN444_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20526.1"
                     /translation="TWGAEIERVYSVRDDATTQQTRQRNRRIRGEPRLRKLADKLLRL
                     DRRVIFVLIALATLIPLLRPIGLPVRISPEVRKVYDYIESLPSGSVFLLSLDFDPASK
                     PELYPMAVALLNHAFKRDLRVIGMTLLVTGTGMAEKVVSGVASEYGKQRGRDYTFLGY
                     SPGGSNVIISMGQDLAAAFPTDHYGQRTMDLPVLNGVNSLRQVNYVISLAAGTPGVES
                     WYVYGKEKYGFELGGGVTAVIAPGLYPFLDTGQINGLIGGLRGAAEYETLIGMKGKAV
                     AGMDAQSATHFVIIGLILLCNLFYFLTRYTTIRFLGSPQDRPGSPREAGR"
BASE COUNT          544 a          679 c          646 g          421 t
ORIGIN      
        1 cgatcgagat catgtaccca ttgctggacc gcgacgaagc tcgatccatc cagcccatca
       61 ccttggcccc gaacgaatgc gacctctagc gcgcgctcat ctttcgggtt ctcggtcccg
      121 tcttcatatc ccgttaaatc acggcctgta ccataggtaa aggcatcaat gacgtggtcc
      181 aggcgaaagg cgggcgccag cgcgtggtta atgcgcctag cccgatgcag taactcaccc
      241 ctatcatccc ccctcaacca acaccacaac gcgaacggtg tggaaggcac gtcaaacccg
      301 ccgccggcat agctcggaaa cgtgcggagg ccgggtacgg ttgccccaag tgcaagcaca
      361 agcgattgac caagcccgac aacagtctgt cgaccgtcgg ctatgtcacg gagcgcccgt
      421 agactcctac gggcgtcgcc gtggtcggtc agagagaaga tgagataccg agcgaccgga
      481 ggggtcgatt ccaggattcc agattgcgca ttattcatag catctccagt acgtgatgtt
      541 cagttaaccc tacgcgtgcg gatcgcttcc agcggtacgc ctgtcctgat cggggtcgga
      601 gagggcgctc gatcgacgct cccataggaa caggccaaag gcgatcagcc ccaggagaat
      661 taacgaggca tagccgtagt cgcgggaggc aaagaggatc aggtcgtcga accggccgat
      721 gaggagcgac attctggcca tcaccgtata gccgaaccct gcgccgaagt tgatcatcaa
      781 aaaatagatc ccaatcctcg ccgcgacctt gatcggccct ttatgctcga tggagaagaa
      841 gaagtagatc aggactgtga tgacgccgac caggatcaga agggcattga attgggccat
      901 cccgaaaggg gactgcccgg aattcaggat cggcttcaat gtcccttgta cttgttgaag
      961 gatgttggag gagatgatgc gggggatggc tacgccggaa ccgaaaccca cgattacggc
     1021 aaacgagatc cggctcagcc agcttacttt cgggatgaaa cgagtcaaga tcaagaggcc
     1081 cagaagggca gcaatcagga accaccattt cccttcctgg gtgactggga catagaggct
     1141 tttgaccatg acgtcgtagt agatccggac gatcgtgtac cccatcgaga caccaacgta
     1201 caggtgctct ccgaatcgga aaaaggggtt gtccttgtac aggaaactga acatgaatag
     1261 ggtgagacca gcggccaccc aggcaccgat gagaacagaa gaggccatca gcgccctgcc
     1321 tcccgcggag agccgggcct gtcctgaggg ctgcctaaga atcggatcgt cgtgtagcgc
     1381 gtcagaaagt aaaagagatt acagagcaga atgaggccga tgatgacaaa atgcgtggct
     1441 gattgtgcat ccattccagc caccgccttc cccttcatcc cgatcaacgt ctcatactcg
     1501 gctgccccac gcaacccgcc gatcaagcca ttgatctgcc cggtatcgag gaagggatag
     1561 aggccagggg caatcacggc cgtcacccca ccacctaact cgaagccgta cttttctttg
     1621 ccatagacgt accagctttc aacacccggg gtacctgccg ccagactgat cacgtagttg
     1681 acctgcctga gcgagttgac tccattgagg acaggaaggt ccatagtccg ctggccgtaa
     1741 tggtcggtcg gaaaggcggc agccaggtcc tggcccatgc tgatgatgac gttggacccg
     1801 ccaggcgagt agcccaggaa ggtatagtcc cgtccccgtt gtttcccata ctcactcgcc
     1861 acaccactca ccaccttttc ggccattccg gtcccggtaa ccaagagggt cataccgatc
     1921 actcgcaggt cccgcttgaa ggcatggttg agcaacgcca cggccatcgg atagagttcc
     1981 ggtttcgagg ccggatcgaa atcaagtgac agcagaaaca ccgatccgga cggaagcgac
     2041 tcgatgtaat cgtagacctt cctcacctca ggcgagatcc ggaccggcaa gccgatcggg
     2101 cggagaagcg ggatcaacgt ggcaagcgcg atcaggacga agatcacccg gcggtccagg
     2161 cgcagcagct tatcggccag cttcctcaat ctcggctccc cacgaatgcg tctgttgcgt
     2221 tgtcgcgtct gttgcgttgt cgcgtcatcg cgaacgctat agacgcgctc aatctcggcc
     2281 ccccaggtac
//
LOCUS       DATMXI010000082         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_187153, whole genome shotgun sequence.
ACCESSION   DATMXI010000082 DATMXI010000000
VERSION     DATMXI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187153"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..807
                     /locus_tag="VN444_01560"
     CDS             115..807
                     /locus_tag="VN444_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWR20527.1"
                     /translation="MAEVVLKGQVRTRVGKGVAKQLRQQRLIPAVVYGGSSGPMAVTV
                     NPVEMLKLLGTGAGENVLITLSLDGDTERSRTVILKELQRDPVRWEPLHADFFEISMK
                     RRIRVQVPLRIVGEAVGVKLRAGILEQHLREVTVECLPGTIPSHIQVDVSHLDLGHAI
                     HVRELTVGEDLRVLDDPARPVVAVMVQRVAVEEAAAATEEKAAEPEVVVGKKEAKEGK
                     EGKQEGGKGKTA"
     gene            826..1407
                     /gene="pth"
                     /locus_tag="VN444_01565"
     CDS             826..1407
                     /gene="pth"
                     /locus_tag="VN444_01565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWR20528.1"
                     /translation="MVVGLGNPGPEYETSRHNVGFRVVETLAGRVGMALNRCRYRSFF
                     MRGSLHGVPVLLVKPLTFMNDSGFAVSRWQQALCLEPSRIIVVHDDLDLPTSQLRIRV
                     GGGHGGHRGVRSVLEAMGSADFLRVKVGIGRPREGQDTVKHVLGPFEEREGDAIQAVV
                     ERAADAVEVLLQESLEVAMNRYNVRGARQARKA"
     gene            1427..1807
                     /gene="rpsF"
                     /locus_tag="VN444_01570"
     CDS             1427..1807
                     /gene="rpsF"
                     /locus_tag="VN444_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWR20529.1"
                     /translation="MNQHEVIIILDPALTEDGVEAEISGLRTVAAKKGGEVLEVQKWG
                     KKRLAYEVKKRREGQYVLMKVDGMGGVVVDIERHFQITEAILKGMVVRAEEPKRKRFK
                     SKSQATLGSSTVITTQEVGDGELQ"
     gene            1791..>2147
                     /gene="ssb"
                     /locus_tag="VN444_01575"
     CDS             1791..>2147
                     /gene="ssb"
                     /locus_tag="VN444_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWR20530.1"
                     /translation="MVSFNKVILLGNLTRDPELRHTPAGMAVCNFDLAVNRSFTAKGG
                     ERREEVCFITVVVWDKQAQSCVEFLSKGRQALVEGRLQQRSWDTPDGQKRSKYEVVAE
                     RVQFIGGRKDAAVSEEE"
BASE COUNT          474 a          455 c          774 g          444 t
ORIGIN      
        1 gaggaatctg tgagctgcct tttcgtctga cggtgaagat agtggatttg atgtgtaggt
       61 gcgggaaggg attccacctg cgcctcgtga gaagctcgtg agaaggaggg tacaatggca
      121 gaagtggtac tgaaagggca ggtccggacg cgggttggca agggagttgc gaaacaactt
      181 cggcagcagc ggctgattcc ggccgttgtg tacggcggta gctccgggcc gatggcggtg
      241 acagtcaatc ccgttgaaat gctgaagctc ctgggaactg gggcgggtga gaatgtcctg
      301 attaccctct ctctggatgg ggatactgaa cgatcgagga ctgtcattct gaaggagtta
      361 cagcgagacc ctgtacgatg ggagccgctg cacgccgatt tctttgagat ctccatgaag
      421 cgaagaatca gggttcaggt cccgctcagg atagtggggg aagccgtcgg tgtcaaactg
      481 agagcaggta ttttagagca gcatctacgc gaggtgactg ttgagtgcct gccagggacg
      541 attcccagtc acatccaggt ggatgtcagt caccttgatc ttggccacgc cattcacgtc
      601 cgcgagctta ccgtggggga ggacctcagg gtgcttgatg atcccgccag gcctgtcgtt
      661 gcggtaatgg ttcagcgggt ggcggttgag gaggctgcgg ctgcaacgga ggagaaagcg
      721 gctgagccag aggtggtggt tggtaagaag gaggcgaaag aaggcaaaga gggtaagcag
      781 gagggcggca agggaaagac cgcctagcgg ggtgcctcgt tgtggatggt ggtgggctta
      841 ggcaatcccg gtccggagta tgaaacgagt cgccataatg tgggatttcg ggtggtggaa
      901 acgttggccg gtcgggttgg catggcactc aatcgctgtc gataccgttc gtttttcatg
      961 agaggctccc tacatggggt gccggttcta ttggtcaagc ccttgacctt catgaacgat
     1021 agcggattcg ctgtgtctag gtggcaacag gccttatgcc ttgagcccag tcgaattatt
     1081 gtggttcatg acgacctgga cctgcctacc tctcagttac ggatcagggt tggcggtggt
     1141 cacgggggcc ataggggtgt ccgttccgtc ctggaggcga tgggcagcgc tgattttttg
     1201 cgagtcaagg tgggaatcgg acggcctcgg gagggacagg atacggtaaa gcacgtgtta
     1261 gggccattcg aggaacgtga gggtgacgcg atacaagcgg tggtagagcg ggccgccgat
     1321 gctgtagagg tgctgttgca ggaaagtctc gaggtggcga tgaaccgata caacgttcga
     1381 ggcgcccgtc aggctcgcaa ggcgtaatct gaaggaggtg aagagagtga atcagcacga
     1441 ggtcattatc attctggatc cagccctcac ggaggatggt gtcgaggcgg agattagcgg
     1501 acttcgcaca gtcgccgcca agaagggggg ggaggtactg gaggttcaga agtggggaaa
     1561 aaagcgactg gcctacgagg tgaaaaagcg acgggagggc cagtacgtcc tgatgaaagt
     1621 ggatggcatg ggcggagtcg tggtcgatat agaacggcat tttcagatta ctgaggccat
     1681 cttgaaaggg atggtcgtca gggctgagga gcccaagaga aagcgcttca agtcgaagtc
     1741 gcaagcgacg ctcggcagta gtaccgtgat aactacccag gaggtcggcg atggtgagct
     1801 tcaataaagt gattttgctg gggaacttga cacgggaccc ggaacttcga catacgccgg
     1861 ccggcatggc cgtgtgtaac tttgacctgg cggtgaatcg ctcttttaca gcgaaaggtg
     1921 gggagcggcg agaggaagtc tgtttcatta cagtggtggt ctgggataag caggcgcagt
     1981 cctgcgtcga gtttctgagt aaggggcgcc aggcactggt cgaaggtcgg cttcagcaac
     2041 ggtcatggga tacgccggat ggccagaagc ggagcaaata tgaagtggtt gcagagcggg
     2101 tgcaatttat cggtgggcga aaggatgcag cggtctccga ggaggag
//
LOCUS       DATMXI010000083         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_147005, whole genome shotgun sequence.
ACCESSION   DATMXI010000083 DATMXI010000000
VERSION     DATMXI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147005"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..2070)
                     /gene="metG"
                     /locus_tag="VN444_01580"
     CDS             complement(157..2070)
                     /gene="metG"
                     /locus_tag="VN444_01580"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWR20531.1"
                     /translation="MSHETFYLTTPIYYVNATPHLGHAYTTILADTMARFQQLRLGPE
                     RVYFLTGTDEHGDKIAQAAAQTGESPQAYADRISGIFQETWRRLGLAPNQFIRTTSEP
                     HKRAVQRLLQQIYDAGDIYFGEYGGQYCFGCERFYTEKELQDGRCPDHQTAPTFIKEQ
                     NYFFRMGKYQDWLIDHIRHHPDFIRPERFRNEVLSFLKDPLEDLCISRPRTRLEWGIP
                     LPFDDKYVTYVWFDALVNYISALGWPDGEAFQRFWPSAQHLIAKDILKPHAIYWPIML
                     KAAGLPLYRHLNVHGYWKIEEAKMSKSRGSVVRPLDLADKYGVEAFRYFVLREMTFGL
                     DASFSEEALVARLNADLANDLGNLYSRVLKLIRRYYDGRIAESPGERGLVDAVRAAVT
                     DVTAATERFAFSEALAAIWNLVSATNKYLVTNEPWKQDPGDARTKAVLFSAAETLRAI
                     AILLWPFLPQTAERMLQGLGAPEPLTSIHFDELLSSSWHLGSRQGRVQEVEPLFPRIQ
                     ATAAKPLPVKTGSTAVLDPVAEQITIEEFRRIDLRVAEVIEATAIPGSKKLVKLRVRL
                     QDEERTVVAGLKEHYPPESWAGKRIILVANLKPTKLMGIASQGMVLAAEDESGRIVLL
                     TPEQPTSSGAKVR"
BASE COUNT          432 a          656 c          640 g          439 t
ORIGIN      
        1 tgcgtatcga tgaacactag cgtagtatcc ccagcacctc tttacatttg ccgttcaccc
       61 tgagccagtc gaagggcaac gttttcaagg cgttccatct gtggttcgac aagctcacca
      121 cgaacggtcc gtaaaggtat ctatgggaca ctacactcag cggaccttgg cacccgagga
      181 ggttggctgt tccggtgtga gcagcacgat tcggcctgac tcgtcttcag cggccagcac
      241 catcccctga gaggcaatcc ccatcagctt ggtcggcttc aggttagcca ccaggataat
      301 gcgcttccct gcccagcttt ccggcggata gtgctctttc aggccggcta cgacggtccg
      361 ctcctcgtcc tgaagcctca cccgcagttt gaccagcttc ttcgagccgg ggatcgcggt
      421 cgcctcgatc acttccgcga cgcgcaggtc gatgcgccgg aactcctcaa tggtgatctg
      481 ttcggctacg ggatcgagaa cggcagtcga tcccgttttc actggtaacg gctttgcggc
      541 ggtcgcctgg atacggggaa agaggggctc cacctcctgg actctcccct gccgtgaccc
      601 gagatgccac gatgatgaaa gcagttcatc gaagtgaatc gaggtgagcg gctctggggc
      661 gccaagaccc tggagcatgc gttctgcggt ctggggaaga aacggccaca ggagaatggc
      721 gatcgcacgg agggtctcgg ctgcactgaa cagaaccgct ttcgtccgag catcccctgg
      781 gtcctgcttc cacggttcgt tggtcaccag gtatttgttc gtggccgaga ccagattcca
      841 gatggccgcc agcgcctcac tgaatgcaaa gcgttccgta gcggctgtca cgtccgtgac
      901 ggcagcgcgc acggcatcaa cgagtccccg ttcaccaggc gactcagcaa tcctgccgtc
      961 atagtatcgc cggatcagct tcaggacacg gctgtagaga ttccccaggt cattggccag
     1021 gtccgcgttc agtcgggcca caagcgcctc ctcactgaag ctggcatcca gaccgaacgt
     1081 catctctcgc agcacgaagt agcgaaaggc ttcaacgccg tacttgtcag ccagatcaag
     1141 ggggcggacg accgagcccc ggctcttgga catcttggcc tcttcgatct tccagtagcc
     1201 gtggacgttc aagtggcgat acagtgggag tccggccgcc ttcagcatga tcggccagta
     1261 gatcgcatgc ggtttcagga tatccttggc gatcagatgc tgggcgcttg gccagaaacg
     1321 ctggaacgcc tcaccatctg gccagcccag agccgagatg tagttaacga gagcatcgaa
     1381 ccagacatag gtgacatatt tgtcgtcaaa ggggagcggg atcccccact caaggcgagt
     1441 ccgaggccgc gagatgcaga ggtcctccag cgggtctttc agaaaggaca ggacctcgtt
     1501 cctgaagcgc tccggccgga tgaagtcggg atggtggcga atgtgatcga tcagccaatc
     1561 ctggtacttc cccatccgaa agaagtagtt ctgctccttg ataaaggtcg gggctgtctg
     1621 atgatcgggg catcggccgt cctgaagttc cttctcggtg tagaatcgtt cgcagccaaa
     1681 acagtactgg ccgccgtact ccccaaaata gatgtccccg gcatcataga tctgttgcag
     1741 caaccgctgc acggcacgct tgtgaggttc gctggtcgtg cgaatgaact ggtttggcgc
     1801 aagtcccagc cgcctccacg tctcctgaaa gatgccgctg atccgatcgg cataggcctg
     1861 ggggctctcg ccggtctgtg cagccgcctg ggcgatctta tcgccgtgct cgtcggtacc
     1921 ggtaaggaaa tagacccgct caggccccag ccgaagctgc tggaagcggg ccatcgtatc
     1981 ggccaggatg gtggtgtagg cgtgccccag atgcggcgtg gcgttgacat aatagatcgg
     2041 ggtggtcagg tagaaggtct cgtgactcat aagttgttac ggtccggcgc agagctaacc
     2101 ccacagccac cactgctgca gccaccgcct ccgccaccgc caccgcaacc catccggggc
     2161 gatgtat
//
LOCUS       DATMXI010000084         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_12848, whole genome shotgun sequence.
ACCESSION   DATMXI010000084 DATMXI010000000
VERSION     DATMXI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12848"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN444_01585"
     CDS             complement(<1..389)
                     /locus_tag="VN444_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20532.1"
                     /translation="MNRRGWLLAIFFTILILPIPARGERVWDNDLKRYLTEQEMTHAE
                     VFLSEDEALKLMFPKSERIRKESLRLTSEKKMQVEERIGWKFPEEVFEVYIGETGTQV
                     DGYALIQNTIGKHKPMTYMVGVDAQGRV"
     gene            complement(386..844)
                     /locus_tag="VN444_01590"
     CDS             complement(386..844)
                     /locus_tag="VN444_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20533.1"
                     /translation="MRARLIAFIGLIAWSGCASLSGSNDQFFVCSYDLVWDAALESVK
                     DRPIQVKDKDKGLIETGWMEMGGQERAYGIFTREGFGNRERARMTVAVKRLNDVTSVS
                     VLENRQRWHLKGGVTQQATKWWPVDPSEEDETAVVTRLNRRLKEKGCIAS"
     gene            complement(909..2396)
                     /locus_tag="VN444_01595"
     CDS             complement(909..2396)
                     /locus_tag="VN444_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20534.1"
                     /translation="MRIRSILGALVITALPFTPVWAENMTPEDIQKLVDEAVNKRMQE
                     LEKREGAMERREPAPPAGQYPVPVGPMTDVKVERPGEEKVPLSFGATGSGRLVYAKPF
                     VSAPKAIVGGYMDIQYRSQRKGSIENGYGGGTNGFDQQRFVPFIYADITEHVKFASEL
                     EIEHGIRGSTGDNEISLEFAHIDYLINEPVNIRAGIVLIPIGKFNLLHDSPLNDLTDR
                     PLVSQLLIPTTMSETGTGFYGTFYPGRTSKLDYELYVTTGPCGYNSDGSPRVNESSGT
                     RNSRQRKCASDDGLDINNGKAVSGRVAFSPMLGVEVAGSSYYGNSSLNGTYNPLSITA
                     IDWTLQRGPFELIGEAAWAYSRGNSRAIQGNQIAFSPGSLLTGIGGNSGPGIPPQRMM
                     GYYIQGNYHFMPAFLTKLSPSRFGEGSTFTAVIRYDRVNTNLDDTNGTGGWGNLEQIS
                     FGLNYRPIEDTVFKISYQYQPMAFNANTDQRIHDSALVVSAATYF"
     gene            3176..>3434
                     /locus_tag="VN444_01600"
     CDS             3176..>3434
                     /locus_tag="VN444_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20535.1"
                     /translation="MAQLTGSFVLEPDVVTFLGTWHFGVDSPKYGRQVMISIIHDEQD
                     HLRAQQFVKDSYPTFDAKPVVVLIPEPSHEQARLFEVMPYPN"
BASE COUNT          771 a          911 c          881 g          871 t
ORIGIN      
        1 acgcggccct gtgcgtcgac gccgaccata taagtcatgg gcttgtgttt gccgatggtg
       61 ttctgtatta gggcatagcc atcgacctgc gttccggtct cgccgatgta gacctcaaaa
      121 acttcttcag gaaatttcca gccgatgcgt tcctctacct gcattttctt ctctgacgtc
      181 agccgaaggg attctttccg aattcgttcc gatttcggaa acatgagttt taacgcctcg
      241 tcctctgaca gaaagacctc ggcgtgggtc atctcctgtt cagtgagata gcgtttgaga
      301 tcgttatccc agactcgttc tcctcgcgca gggatgggaa gaatgagaat ggtgaagaat
      361 attgccagga gccagcctcg acgattcatg aggcaataca gcctttctct ttcaatctgc
      421 gattgagtcg ggtcacgact gccgtctcgt cttcttcaga tggatcaaca ggccaccact
      481 tggtggcctg ttgtgtgact ccacctttga ggtgccaccg ctgccggttt tccagcacac
      541 tcacagaggt gacgtcgttc agccgcttca ctgccaccgt catacgagca cgttcccgat
      601 tcccaaaccc ttcgcgggtg aatatcccgt aggcacgctc ttggcctccc atctccatcc
      661 agccggtctc gatcagccct ttgtccttgt cttttacctg tattggtcgg tctttcacag
      721 attccaaagc ggcatcccat accaggtcat acgaacagac aaagaattga tcgttgctgc
      781 cgctcaggga agcacagcca ctccaagcga tgagtcctat gaaggctata agtcttgctc
      841 gcacgtgatg ttatgctccg tggtggattc tgcgaaccct cccgcaagag ggttcgcaga
      901 ccactggttt agaaatatgt cgcggctgaa actaccaaag cgctgtcgtg gattcgctgg
      961 tctgtattgg cattaaaggc catgggctga tattgataac tgattttgaa gaccgtatct
     1021 tcgatgggcc gatagttgag accgaaagaa atctgctcca gattacccca gccgcctgtc
     1081 ccattcgtat catccaagtt ggtgttcact ctgtcgtacc ggatcacagc ggtgaaggtc
     1141 gatccctcgc caaacctgct cggggaaagc ttggtcaaga acgccggcat gaaatgatag
     1201 ttgccctgga tgtagtagcc catcatacgc tgcggaggga tgccggggcc gctgtttccc
     1261 ccgatgcccg tcagtagaga tcctggggaa aatgcaatct gatttccttg aatggcacgt
     1321 gaattccctc tcgaataggc ccaagcagct tcaccgatga gttcgaaggg acctcgttgg
     1381 agcgtccagt cgatagctgt aatgctaagg gggttgtagg tgccattgag ggacgaattg
     1441 ccatagtagc ttgatcctgc cacttcaact ccgagcatgg ggctgaaggc cacacggcct
     1501 gaaaccgctt ttccgttatt gatgtccagt ccgtcatcgg atgcacattt gcgttgacgg
     1561 gagtttctcg tgccgctcga ctcattgacg cgcggggaac catcactgtt atagccgcaa
     1621 gggccggtcg tgacatagag ctcatagtcg agcttactgg ttcgtccagg gtagaaggtg
     1681 ccatagaagc ccgtgcctgt ctcggacatg gtagtaggaa tcagaagctg gctgacaagc
     1741 ggacgatcag tcaagtcatt caacggcgag tcatgaagca agttaaactt gccgatggga
     1801 atgagcacaa ttcccgctcg aatattcact ggctcattga tgagatagtc aatatgggca
     1861 aattccaagc tgatctcgtt gtcgccagta ctccctctaa tcccatgctc aatctcaagt
     1921 tcagatgcga acttcacatg ctcggtaatg tccgcataga taaatgggac gaaccgttgt
     1981 tgatcaaacc cgttcgtacc gcctccataa ccattttcga tactgccttt gcgttgggaa
     2041 cgatactgaa tgtccatata cccaccgaca atggcctttg gtgccgagac aaacggtttg
     2101 gcatagacca accgtcctga accagtggcg ccgaacgaga gcggcacctt ttcttcgcca
     2161 ggccgctcaa ccttgacatc cgtcatggga ccgaccggca cgggatattg tccagccggt
     2221 ggtgcaggtt ctcgacgctc catagctcct tcccgcttct caagttcctg catccgcttg
     2281 ttgacggctt cgtcgactaa tttttgaatg tcctctgggg tcatgttctc agcccaaacc
     2341 ggagtaaacg gcaaagctgt aatcacgaga gccccgagga ttgacctgat cctcattgca
     2401 aacctccatg gataccatat tgtgataggt ctgactcggg actcgatggt ctctggtcgt
     2461 tattgttgac aacgatagat cgtattttgg tggattggtg cgaagcctcc tttcgttgaa
     2521 gagcgcatca tgatgcgacc ctgctccatc ctttgataga agagaaaggg atgacaacga
     2581 tacgtttgca gcgtttgcac ttaagttcca gcccctgtcc atgcacctta gcgatcagtt
     2641 gaccgcactc gcagcgagtc tcactgcagt ctgcagagtg gtcgaccgga gatatcgcac
     2701 tagccattat tggggccgcc tcatccaagt gaaattgaga acgattatta atatcctatt
     2761 tttggattcg aagtcaagcg gtgaacgtgt tcgccaggat gcaggatgta ttggcatcgc
     2821 aggaggccta tactatctgt gcatgcttca gaagctgttg agacagcagt ggtggtggca
     2881 atctctctgt gcattgagct tgaatctctc aggctgtgcc acagaacttc ctgtaacagt
     2941 ggcagaggct gatgtatcgg gggctttggt agtggggcgg gtcgccacga ccatcaccgg
     3001 gcactcgcaa cgagtgtatc cacctgaact ccgttccttt gagctgatca atacagagaa
     3061 ccaggaacga tttaagatat atgtgaaatc tgcagacggg tatttttcgc tttccctccc
     3121 tcccggacgc tatgcaatta atcgtgtaca agtcagtgaa ggtccattct tgtccatggc
     3181 gcagctcacg ggaagctttg tgctcgagcc tgacgtagtg accttcctcg gtacgtggca
     3241 ttttggagtg gactctccga aatacggacg ccaagtgatg atctccatca tccatgacga
     3301 acaagatcat ctgcgagccc aacagttcgt taaggattca tatcccacct ttgacgccaa
     3361 gcctgtggtg gtgctgatac cggagccatc gcacgagcag gcccggttat tcgaagtgat
     3421 gccctatcca aact
//
LOCUS       DATMXI010000085         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_208765, whole genome shotgun sequence.
ACCESSION   DATMXI010000085 DATMXI010000000
VERSION     DATMXI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208765"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..980
                     /locus_tag="VN444_01605"
     CDS             465..980
                     /locus_tag="VN444_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HWR20536.1"
                     /translation="MTKLMKIPFLLFIGMVVSSMFTTTVALADCYRDCMDASGCWSTE
                     PGVNVSYCSDTQARCSSDCLNKGPSGRGKIYGAIAYSKENGAHGYSHGWTNQKKAETV
                     ALKNCSEHGSGCESVVWFHNSCGAVASDGRHVTWGQADSAQVAMKQALDKCNKSFFRG
                     KCKNVVSHCSG"
     gene            complement(1043..1174)
                     /locus_tag="VN444_01610"
     CDS             complement(1043..1174)
                     /locus_tag="VN444_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWR20537.1"
                     /translation="MLVIPAIDLKGGQVVRLSQGDPLRQIAYSAARSTYSTRTMMAK"
     gene            complement(1175..1819)
                     /gene="hisH"
                     /locus_tag="VN444_01615"
     CDS             complement(1175..1819)
                     /gene="hisH"
                     /locus_tag="VN444_01615"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWR20538.1"
                     /translation="MIAIIDSGVANLRSVQKGFERVEADARIVEYPLTLRDASGIVLP
                     GVGAFADGISKLRDGGFVEPLLRAIEEGKPVLGICLGLHFLFSESEEFGHHAGLNVIK
                     GRVVRFTNNLPVPPFRKGGVGGFLKIPHMGWNRIRIEQQAPIFKGIPDGAFFYFVHSY
                     YVQPEEKSVIAATTEYGLRFTSVLWRDNLFACQFHPEKSQALGLQLLKNFASLT"
     gene            complement(1884..2471)
                     /gene="hisB"
                     /locus_tag="VN444_01620"
     CDS             complement(1884..2471)
                     /gene="hisB"
                     /locus_tag="VN444_01620"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWR20539.1"
                     /translation="MARTARVCRKTLETDVAVELNLDGVGRHTLQTQIPFLDHMLAQL
                     ATHGRFDLSIEARGDLQVDLHHTVEDVGIALGEAFAQAVGDKRGIRRFASALVPLDEA
                     LARVVVDISGRPYLVYQAPQLQGEIGSFDVTLVKEFMRALAMNMKVNLHVEVLHGENL
                     HHCVEAIFKALAKSLDQATALDARSADVPSTKGSL"
     gene            complement(2529..3644)
                     /gene="hisC"
                     /locus_tag="VN444_01625"
     CDS             complement(2529..3644)
                     /gene="hisC"
                     /locus_tag="VN444_01625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWR20540.1"
                     /translation="MKTPRLLDLVKPEVLSLTAYRAEEPRPDLIKLDANESPFPLPEE
                     LRRELRHALDQVAVHRYPDPKEDRLRSVLAAQLQVASGALVLGNGSDELIQLLLLATS
                     GPGTTILAPVPTFSMYELIARAQGLRFEGVPLGSRFELDLPRLIDTIERRNPKVIFLA
                     TPNNPTGGVLSEAEICKILAVAPGLVVVDEAYGPYAGRTMLPHLVDQERLVILSSLSK
                     IGLAGLRIGYLVAHPALTAELEKVRLPFNLNAFSQAAAAVLLKHQEWIDASVREVVRE
                     RVRVMNRLVALPGVEAFPSEANFILFRTTSPGNDVFEAVLGQGILVRNLGSVPGLQNC
                     LRVTVGTKSENDRFLDALTKALKPPTPPFTKGGHGGI"
     gene            complement(3641..>4003)
                     /locus_tag="VN444_01630"
     CDS             complement(3641..>4003)
                     /locus_tag="VN444_01630"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWR20541.1"
                     /translation="VKGLDEATALVNEIAPEHLELLVKDPWGLLPQIRHAGAIFMGGA
                     SPEVVGDYLAGPNHVLPTGGTARFASPLSVDDFQRRTSLIAFSRQKLEALEPTLVELA
                     RLEGLEAHARAASIRRLA"
BASE COUNT          866 a         1169 c         1123 g          845 t
ORIGIN      
        1 agtcggtcga tgtacccaag ctcctgcgca tccaagcttt ccacgagaag gtccgctaac
       61 cgcgcccgcg attcgatcgg tagggtcatg gcctgctcgg ccagcttttc cacagttgta
      121 gccatggcca aaagatactc caactctcct caattctcaa gcggcagaca ttagcgtgta
      181 ggaactttca tcgcggtttt tctccatcgg gctgagtata gacaaggtgg tgcatggtgt
      241 caatgggggg aaatcgagga gcttttgggg gaggcagttc cagggagacc aatccgtttc
      301 ttgtgatagc gcttggtctt attgaagtac ttcttaattg agaggtcgtg tagtattatc
      361 agtaagagtt taacggtgtc tcgtatgtag caccatttat ccttatcgat tacggcagca
      421 ttgcattttc aggcttcact ctcttttaat aaggaactct acagatgaca aagctaatga
      481 aaataccctt tctccttttt attggtatgg ttgtgtcgtc catgttcacc acaactgttg
      541 ccttggccga ttgttaccgg gactgtatgg acgcgtctgg ctgttggagt acagaaccag
      601 gggttaacgt aagttattgc agcgacaccc aggctcgatg ttcatcagat tgcctcaata
      661 aaggcccaag cggccgcggt aaaatctatg gcgccatcgc ctacagcaaa gaaaacggag
      721 cgcatggcta ctctcacggc tggacgaacc agaaaaaagc cgaaacagtc gcgcttaaaa
      781 actgctcgga acacggatcg gggtgcgaat ctgtggtgtg gtttcacaat agctgcggcg
      841 cagtggcaag cgatggcagg catgtgacct gggggcaagc tgattctgcg caggtcgcca
      901 tgaagcaagc cctcgacaaa tgcaataaaa gcttttttag gggaaagtgc aaaaacgtgg
      961 tatcccactg ttcaggatag accacgccat cgtttaaaaa gtctcggccg ccccatcaga
     1021 catgccatta attcatcggg gcttattttg ccatcatcgt tcgcgtcgag tacgtcgaac
     1081 gagcggcgga ataggcgatt tgacgcaggg gatcgccttg cgacaaccga acgacttggc
     1141 ccccttttag gtcaatcgct ggaatgacta gcatctacgt gagggaggcg aagttcttca
     1201 acagttgcaa acctagcgct tggctctttt cggggtgaaa ctggcaggca aagaggttat
     1261 ctcgccacag cactgaggtg aatcggaggc catactcggt cgtggcggcg atcacgcttt
     1321 tctcctctgg ttgcacatag taggagtgga cgaagtagaa aaatgcccca tcgggaatgc
     1381 ccttgaagat cggggcctgc tgttcgattc ggatccgatt ccaccccatg tgcgggattt
     1441 tgagaaatcc ccccaccccc cctttacgaa aggggggcac ggggagatta ttggtaaatc
     1501 ggaccacccg gcccttgatg acgttgaggc ccgcatgatg cccgaactcc tcactctcac
     1561 tgaacaggaa gtgcagtccc aggcagatgc ccaggaccgg cttaccctcc tcgatcgctc
     1621 gaagtagcgg ctcgacaaat ccaccatccc gaagcttgct gatcccatcg gcaaacgcgc
     1681 ccaccccagg aaggacaatg ccggatgcgt cccggagcgt cagcggatac tctacgatcc
     1741 tggcatcagc ctcaacacgc tcgaacccct tctggacgct ccggaggttc gcaacaccgg
     1801 aatcgatgat ggcaatcata gttacccgcg ttcaaagttc aacgttctag gctcaaggtt
     1861 ctaatcgcga cactgagaac tgtttataga ctccctttgg tcgagggaac gtcggcactt
     1921 cgcgcatcga gagctgtcgc ctggtcgagg ctcttggcta atgccttgaa aatcgcttcg
     1981 acgcagtggt gcaggttctc accgtgcagg acctcaacat ggaggttcac cttcatattc
     2041 atggcgagag cgcgcataaa ctccttcacg agggtgacgt cgaaactccc gatttcccct
     2101 tgcagttgcg gcgcctgata gaccagatag gggcggccgc tgatgtcaac caccacgcgg
     2161 gccagcgcct cgtccagcgg gacaagtgct gaggcaaacc gccggatgcc ccgcttgtcc
     2221 cccacggctt gggcgaacgc ttcgccgagc gcgatcccga catcctccac ggtgtggtgc
     2281 agatcgacct ggaggtcgcc ccgcgcctca atgctgaggt cgaagcggcc gtgcgtcgcc
     2341 agttgggcca gcatgtgatc gaggaagggg atctgggtct gaagtgtgtg tcggcccacc
     2401 ccgtccaggt tgagctccac cgctacatcg gtctctaagg tcttcctgca caccctcgct
     2461 gtcctggcca tcatctgatc tccgtctgct ccattgcact ttgtccttcg cccttcgcac
     2521 cacgcacctc aaatcccccc gtgcccccct ttcgtaaagg ggggggtggg gggtttcagc
     2581 gccttcgtca atgcatccaa aaaccgatca ttctccgatt tcgtgcccac agtgactcgc
     2641 aggcagtttt gcagacccgg cactgatcct aaatttcgca ccaggatgcc ctgccccaac
     2701 accgcttcga atacatcgtt ccccggagat gtcgtccgga aaagaatgaa gtttgcctct
     2761 gacgggaagg cctcaacacc ggggagcgcg accaagcggt tcatcaccct cacccgctcc
     2821 cggaccacct cacgaacgct cgcatcgatc cactcctggt gcttgagcaa cacggcggcg
     2881 gccgcctggg agaaggcgtt gaggttaaac ggaaggcgga ccttctccag ctcggctgtg
     2941 agtgcaggat gggcaacaag gtagccgatg cgcagaccgg ccagcccgat tttggaaagg
     3001 gagctgagga tcaccagccg ttcctgatcg accaggtgcg gcaacatcgt tcgcccggcg
     3061 tacggtccat aggcttcgtc cacaacgacc aatccgggag caacggccaa aatcttgcag
     3121 atctccgcct cagagaggac gccgccggtt ggattgtttg gtgtagccag aaagatgacc
     3181 ttggggttac gtcgctcgat ggtatcgatc agcctgggca ggtcaagctc gaagcgagat
     3241 ccgaggggta ctccttcaaa gcgcagcccc tgagccctcg cgatcagctc atacatcgaa
     3301 aaggttggaa ctggagcgag aatcgtggtc ccaggtcctg aagtcgccag taataacagt
     3361 tgaatcagct catcagaccc attgcccagg acaagtgccc ccgacgccac ctggagctgt
     3421 gcggccagaa ccgatcggag ccgatcctcc tttggatcgg ggtagcggtg tactgccacc
     3481 tgatccaacg cgtggcggag ctcccggcga agctcctccg gaagagggaa cgggctttcg
     3541 ttggcatcga gcttgataag atcgggtcgt ggttcctcag cccgatacgc cgtgaggctc
     3601 aggacttcgg gttttaccag atcgagaagg cgtggcgtct tcatgcgagc ctcctgatag
     3661 aagccgccct cgcgtgggcc tcaagcccct ccaagcgggc cagctcgacc aatgttggct
     3721 ccagcgcctc cagcttctgc cggctgaagg cgatcagcga ggtccggcgc tgaaaatcgt
     3781 ccacggaaag gggggaggcg aagcgcgccg tccctccggt cggcaggaca tgattcggcc
     3841 cggccaggta atcgcccacc acctccggac ttgcgccccc catgaagatg gcgcccgcgt
     3901 gcctgatctg gggcaatagt ccccagggat ccttcaccag cagttccagg tgttcgggcg
     3961 cgatctcatt gacaagggca gtggcctcgt ccagcccctt tac
//
LOCUS       DATMXI010000086         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_280199, whole genome shotgun sequence.
ACCESSION   DATMXI010000086 DATMXI010000000
VERSION     DATMXI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280199"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /gene="der"
                     /locus_tag="VN444_01635"
     CDS             <1..1330
                     /gene="der"
                     /locus_tag="VN444_01635"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWR20542.1"
                     /translation="PYPLRIPIVAIIGRPNVGKSTLFNRILGKRTAIVDDVPGVTRDR
                     NYADATYRNRPFRLVDTGGLDPSASEGMLVLIKRQSELAIAEADILILLMDGRTGLTT
                     PDQEVVRLLRGTPKPLFVVVNKIDQPKVEMLVADFYQLGTETLYPISAEHGIGVSELL
                     DAIYPFLPAPDENPEQTAMPRIAVVGRPNVGKSTLVNAVLGEDRVVVSDVPGTTRDSI
                     DSIALHRGRRYVFTDTAGIRRRGKIDRGIEGYSVVRSHLAIGRSDLGILLLDATEGVT
                     EQDTKIAGIIIKQGRACILLVNKWDLREGDNQARQEVELELRRRFPFLTWAPVLFASA
                     AHPDSLDQLFPTIDRVVAAFSKRIPTGALNKFLQDILTTHPLPVRKGKPTKITKSAFM
                     TQVTTQPPVFALFVGHPDNITPAYLRFLENQLREEYGFEGTPIRILVRKK"
     gene            2102..>2616
                     /locus_tag="VN444_01640"
     CDS             2102..>2616
                     /locus_tag="VN444_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20543.1"
                     /translation="MRDSFPSRLQRRAIEALCAGVVVATLCGPIYAEEPVGPAETASP
                     QAIESLAQSPAATAQAPVDPFIKLQGSVWRTKAGIVFLKTPIGLLTLTSKTTLKDLKA
                     SQEVRFWLHDRHSIVEIRKRADGSLVHRYLSGPMTSGTDVPRTLRWWGPDGDQTVHVG
                     TQEERLTDYREG"
BASE COUNT          544 a          787 c          689 g          596 t
ORIGIN      
        1 cccttacccc ttacgcattc cgattgtggc cattatcggc cgccccaacg tcggcaagtc
       61 gaccctcttc aatcgtattc tcggcaagcg aacggctatc gtggacgatg tccctggtgt
      121 cacgcgggat cgtaactatg ccgatgccac ctatcggaat cggccgttcc gcctggttga
      181 tacagggggt ctggacccgt ctgcctctga aggcatgttg gttctcatca agcgtcaatc
      241 cgagcttgct atagcggaag cggacattct gatcctgctc atggatggtc gcactgggtt
      301 gacgactccg gaccaggaag tggtccgtct cttgcgcggg acaccgaagc cgttatttgt
      361 cgtggttaac aagatcgacc agcccaaggt tgaaatgctc gtggcggact tctatcaatt
      421 gggcacagag acgctctatc ccatctctgc ggagcatggt atcggtgtct ccgaactgct
      481 ggatgcgatc tacccgttcc tcccggcccc agatgagaac cctgaacaga cggccatgcc
      541 tcgcatagct gtcgtggggc gcccgaatgt cggcaaatcc accctggtca atgccgtact
      601 cggggaagat cgagtcgtag tcagcgatgt gccgggaacg actcgggatt cgattgactc
      661 catcgcactc catcggggac ggcggtatgt cttcaccgat acggctggaa ttcgtcggcg
      721 tggaaagatc gacagaggga tcgaaggcta tagtgtggtc cgatcgcacc tggcgatcgg
      781 tcggtccgac ctcgggatct tgttgctcga tgctacggaa ggcgtgactg agcaggatac
      841 caaaatcgcc gggatcatca ttaagcaggg aagggcctgc atcctcctgg tcaataaatg
      901 ggaccttcgg gaaggcgata accaggcccg gcaggaggtc gagctggaac ttcgtcgtcg
      961 atttcctttt ttgacctggg ccccggtcct cttcgcctct gcagctcatc ccgactcgct
     1021 tgaccagctc tttcctacca ttgacagggt agtcgcagct ttctcaaaac ggatcccgac
     1081 cggagccttg aacaagtttc tccaggacat tctcaccacc cacccgctgc cggtgcgaaa
     1141 aggcaagccg acgaagatca ccaaatcagc cttcatgacc caggttacga cacaaccgcc
     1201 ggtctttgcg ttgtttgtcg ggcacccgga caatatcaca cccgcctacc tgcgctttct
     1261 cgagaatcaa ctccgcgagg agtatggatt cgagggaacc ccgattcgca tactggttcg
     1321 aaagaaatag ggcgtggtcc aatttccgcc gctcttcgac cctaattgtg cgccaacggc
     1381 ctctgagtgc cacgttatgg tcggtattcg cagggtagtt atcctttttg catctcaggt
     1441 ttgggtgcaa ctcttgcaca acccgaagaa catttttccg aaaggatctc tatgccgcga
     1501 tcagccctgc aaaaccaagg taactctccg cgactcctgc ttccttcccc tgactcaggc
     1561 atgatcgtat tttcttcgtc cgctcgtatc ctgcacatga atggatcagc tcgtgcgctc
     1621 atggcgttgt tcggtacgtc ccacgagctc tggccgcaga tggcgccaga atctatgcca
     1681 tctatcctca cggaattctg cggggatgtc ctggtacaac ttcgacaccg ggtcgaggca
     1741 caggactggg cacagttcga aatgcgccgc atatgccata tggtcacacc agcgctcttg
     1801 ctcacgggat ttggtgtgcc caatgcaacc aatcgagaga ttcgggtcat cctgaccctc
     1861 acccctctgt catcaatgcc ttgcacagct cagaacccac ggcattccca attatcggcc
     1921 gacatactcg cttcagtcag tcacgcactt tcatagggca acgccttctg aaccactgcc
     1981 gtgaccggtt ttcaccttga ctccctctga gacatcatgg tattcgctca atctggtgac
     2041 ggatttcaga tttagaggga ctcttcgccc tgcggattgc cacgacaaac gaggtccggg
     2101 gatgcgcgat tcctttccca gcaggcttca gcgacgtgcc atcgaggccc tctgcgccgg
     2161 ggtggtcgtc gccactctct gcggtcccat ctatgccgag gaaccagttg ggccagccga
     2221 aacggcttca ccccaagcaa ttgaatctct tgctcagtct cctgctgcga ctgcccaggc
     2281 cccggtcgat ccctttataa aattacaagg atctgtgtgg cggaccaagg caggtatcgt
     2341 ctttctcaag accccgatag gattattaac gctcacttcc aagacaactc tgaaggatct
     2401 caaagcctcg caagaagtac gtttttggct gcacgaccgc cattccatcg ttgagatccg
     2461 aaagagggcc gacggatcgt tggtccatcg ctacctcagc gggccaatga cctccgggac
     2521 cgatgttccg agaacgttac gctggtgggg gcctgacggt gaccagactg tccatgtcgg
     2581 cacacaggaa gaacgcctca ccgactatcg cgaggg
//
LOCUS       DATMXI010000087         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_234635, whole genome shotgun sequence.
ACCESSION   DATMXI010000087 DATMXI010000000
VERSION     DATMXI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234635"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VN444_01645"
     CDS             <1..869
                     /locus_tag="VN444_01645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWR20544.1"
                     /translation="HELVHREEASMVGFHIAWNLIFGVPFLLHSLLYRSHLDHHHPRK
                     FGTPAEGEYLPLASAPIRETLLYLAQIPAVPLLAAFRFLVLVPLSFLYPPSRRWLLEQ
                     WSSYTINPYYRRVIPPTEPLGLWAVLDLLCLLWLVVVIGLILKGFIPWTMVGMVYGLS
                     ISTIALNWVRTLAAHRFLNTGGNMTYVEQIEDSQTIEGRSLLTLLMFPIGLRYHALHH
                     LFPLMPYHSMGEAHRRLMSALPEDSPYKKGIFPGLWAALRELLRGARLAGRAGHNAMQ
                     AWRHPGTATYAR"
     gene            859..1821
                     /locus_tag="VN444_01650"
     CDS             859..1821
                     /locus_tag="VN444_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20545.1"
                     /translation="MRGRREAWTQLASSIEQHSARPVAPGVRALADTLLASYGNAVVA
                     ILAYGSCFRNQTDEGLVDLCVLVDSYRSLRGTLWQRWLYRTLPPTVFSMEIECGGRAI
                     RTKYTVISCTDFERGTSVRCFHSHLWGRYAQPTGVLYVRDEHGAQRVFKALAQAVVTF
                     IGCVLPVLPSTFNARELWQQALTLSYGTELRPERADAVVRLVDDSLDYYEQVTRIGMG
                     CLQLDVRLVEEGGPSRYHAVIPRSTRSITGLAWRIRKVQGKTLNILRLLKGVFTFQGG
                     ASYILWKIERHSGMRAEVPPHLQHHPWLATALILGKLYWRRAYR"
     gene            1826..>2278
                     /locus_tag="VN444_01655"
     CDS             1826..>2278
                     /locus_tag="VN444_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HWR20546.1"
                     /translation="MMHRALSVVSGLWLTCLLLLSWGAPGRIEAIERACDRTSTAIDV
                     RVRGVRSDRGNIMFVLYGDNPDDFLVKGKKVLKQRIPAKRGIVAFCVIAPKAGVYAAA
                     VYHDENGNGKFDRNRIGLPSEGGGFSNNPSLFLITPSHEQVAFHVSNSQ"
BASE COUNT          439 a          638 c          651 g          550 t
ORIGIN      
        1 tccatgagct tgtccacagg gaggaggcct ccatggtagg atttcatatc gcctggaatc
       61 ttattttcgg agtgcctttt cttctccatt cactcctgta ccgcagtcat ctcgaccatc
      121 atcatcctcg taagtttggc actccggctg agggagagta tctccctctg gcctcggcgc
      181 ctatccgaga aactctgctc tatctcgcgc agatcccggc cgtgccgctc ctggccgctt
      241 ttcgcttcct ggtcttggtg ccgctgtcat ttctctatcc cccctctcgg cggtggctcc
      301 tggaacaatg gtcctcgtat accatcaatc cctactatcg gcgtgtcatc ccaccaacgg
      361 aacctctcgg tctctgggcg gtactggacc ttctctgtct gctgtggttg gtcgtcgtca
      421 tcgggctgat tctcaaagga tttatcccgt ggacgatggt tggaatggtc tatggtctgt
      481 cgatctctac aatcgctttg aactgggttc gcacgctggc tgcgcaccgt ttcctgaata
      541 cgggaggcaa catgacctac gtggagcaga tcgaggactc tcagactatc gagggacgtt
      601 ccttattgac actgctcatg tttccaattg gattgcgtta tcatgccctc caccatctgt
      661 ttccactgat gccctatcac tctatgggcg aggcccatcg ccgcttgatg agcgcacttc
      721 ccgaggactc gccgtacaag aaagggattt ttcccggttt gtgggctgcg ctccgcgaac
      781 tgttgcgagg cgcaagacta gcaggaaggg ccgggcacaa tgcgatgcag gcgtggcgtc
      841 acccaggaac ggcgacgtat gcgcggtaga cgggaagctt ggacgcagct cgcctcttcc
      901 atcgagcaac atagtgcgcg acccgttgcg cctggagttc gcgccctggc tgacacgctt
      961 ctggcgagct acggcaacgc cgtagtcgcg attcttgcgt atggctcctg cttccgaaat
     1021 cagacggacg agggacttgt cgatctttgt gtgctggttg acagctaccg gtctctccgg
     1081 gggaccttgt ggcagcgctg gctgtaccgt accttaccgc cgaccgtgtt ttctatggag
     1141 atcgagtgtg gtggtcgtgc catccgaaca aagtataccg tgatctcatg tacggatttc
     1201 gaacgaggaa cctcagtgcg ctgttttcat tcgcacctct ggggaaggta cgcacaacct
     1261 accggggtac tgtacgttcg cgatgagcat ggagcccagc gggtgtttaa ggcattagcc
     1321 caggctgtcg tgacatttat cggttgcgtt cttccggttc tgccatcgac atttaatgct
     1381 cgcgaactgt ggcagcaggc gctgaccctg agttatggca ccgaactacg gcctgaacgg
     1441 gcggatgccg tagtgcggtt agtcgatgat tcgcttgatt actatgagca ggtcacccgc
     1501 atcgggatgg ggtgtctgca actcgacgta cggctggtcg aagaaggcgg gccaagtaga
     1561 tatcatgcgg tcataccccg ctctacccgc tccatcaccg gccttgcatg gcgaatccgg
     1621 aaagtgcagg gaaaaactct gaacatcctg cgcctcctca agggggtgtt cacgtttcag
     1681 ggcggtgcga gctatatcct gtggaagatc gaacggcatt ccggtatgcg ggcagaagtg
     1741 ccaccgcatc tacaacatca cccatggctt gcgacggcgc tgatcctcgg taaactctac
     1801 tggcgcaggg catatcgata gcagcatgat gcaccgagca ttgtcagtag tatcgggcct
     1861 ctggcttact tgcctgctgt tgctctcatg gggtgcacct ggccggatcg aggcgatcga
     1921 gcgggcatgt gaccgcactt ctaccgccat cgatgtccga gtgcggggag tccggagcga
     1981 tcgcgggaat atcatgttcg tcctgtatgg cgacaatccg gatgatttcc tcgtgaaagg
     2041 caagaaagtc ttgaagcaac gcattccggc gaagcgcggg attgtcgcgt tttgcgtgat
     2101 tgcaccaaag gcaggcgtgt acgccgctgc cgtgtatcac gatgaaaacg gcaatgggaa
     2161 atttgacagg aaccggatcg ggttacctag cgaaggcggc ggattctcga acaaccctag
     2221 cttattcctg atcacaccca gccatgagca agtggcattt cacgtctcga atagtcag
//
LOCUS       DATMXI010000088         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_41761, whole genome shotgun sequence.
ACCESSION   DATMXI010000088 DATMXI010000000
VERSION     DATMXI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41761"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VN444_01660"
     CDS             <1..327
                     /locus_tag="VN444_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20547.1"
                     /translation="GQHRLTIVVDEADWANHRLRVQLVDVSTKGELLNSNVAFTLTHF
                     DFPLTDNTLFPDGNRIALALSSVHAEGGLKSVALKLIWFPKDYFTPRERPLDSSEFLK
                     LVGKKT"
     gene            891..1598
                     /locus_tag="VN444_01665"
     CDS             891..1598
                     /locus_tag="VN444_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20548.1"
                     /translation="MTGRVTALICGVMGLVLITGCRSRSELRLKAVNVPASAIYCLFD
                     PSCAVTFTNSSTTPIPISSGGTSFLHARSFAGKPGTPASGLYGYEYRIDLSKAVETIV
                     DVEDVGKVKYMPCLQTIALEFGPIIDTLDYDGDGKAGDLAYVVASGGPGKIGLDSFER
                     YHNMLTFRFDSPICAGGPHSEGDSTYFFGLVSARPSRFVTATIKETTGLSSVSPKMKK
                     NLRHKVQVRAPQIGTAE"
     gene            1906..>3034
                     /locus_tag="VN444_01670"
     CDS             1906..>3034
                     /locus_tag="VN444_01670"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR20549.1"
                     /translation="MPPALQSQAEVGKRALVPLGPRMVTGYIVGLHTFSDQPSAVRHQ
                     ASELKPIDTILDPEPLLDHHMLELTRRVADYYLTSWGLVIRAALPPGIDRRTARTVEL
                     IESPDLFPHALNGERLAQKAGKLGPIQQQMLASLQAQRRMLLSSLKQRWPNEEVDRLI
                     RMLVRQNLAKVEYRECLPLIRPAYRSLLSLAVDQASAEAELAALRHRAPRQASLLERL
                     LQSDSKLTSAEATVLAGASGVRALIAKGFIHRVTEEVERSPWEETTAATGSWPEPNSA
                     QQIAIDGLLEGLPSHTFVPALLHGATGSGKTEVYLRVIGEVVRQGRQALVLVPEIALT
                     PVTADRFRSRFGDRVALLHSGLSPGERLDQWHRIKRGMADIV"
BASE COUNT          634 a          908 c          871 g          621 t
ORIGIN      
        1 gggcaacacc gattgaccat tgtcgtagac gaagccgact gggccaacca tcggttgagg
       61 gtgcagctcg tcgacgtcag cacaaaaggg gagttgctga actcaaacgt agcattcacc
      121 ctcacccact tcgactttcc ccttaccgac aacaccctct tcccggatgg caacaggatc
      181 gcgctcgctc tctccagcgt ccacgctgag gggggactaa aaagcgtagc gctcaagctc
      241 atctggttcc caaaggacta ttttacgcct cgggaacgac ccctcgacag tagtgagttc
      301 ctgaaactgg tggggaagaa aacatagggt acgaattcag tggcatcttc caggggagcc
      361 cgtccaggct acgaatacat cctcggcagg gactggagcc ctgctctggt acgcttgttc
      421 atccgggcat cgaggcgccg atgcactccg ccagcgcctc ggccaatgca caccgtcact
      481 ggcaactctt cccgcagccc tgcttctgcc tgtaggcgta ctaccgggct gttcgccacg
      541 cgccgtaccg gttcaagaat acgctggtgt gtctcgacgc tatcgtggtt gcccactgtg
      601 ttgcctgatt tcgatagaac tcattttcgt cgtacgcaag gggccatcaa gttgctataa
      661 tagagccgga atgtgagaga gctggatcag gacgtctcct cctctaaaag aaggcgggag
      721 agtggaggaa ggcgaacgta gtgcgcgggc aacgaacaga gttggcaaca tgctcatctt
      781 ttcagctcgg acttgagaaa ccgctgagcc ctgtgagccg atcgtaaaga aaacagatga
      841 atgcttcttg acgcccaggc gtgactatga gggaaagggg gagtacggag atgaccggaa
      901 gggtaactgc cttgatctgt ggggtgatgg gtttggtcct tattacggga tgccggagca
      961 ggagcgaact ccgtctcaag gccgttaatg tgcctgcttc agccatttac tgcctcttcg
     1021 atccatcctg cgcggtgacc tttaccaaca gcagcaccac tcctatcccg atatcgtccg
     1081 ggggaacgag tttcctccat gcccgctcct tcgcgggcaa gccgggtacg ccggcgagtg
     1141 gtctgtacgg gtatgagtac cggattgacc tctcgaaggc cgttgagacc atagttgatg
     1201 tcgaggatgt cggtaaggtt aagtacatgc cttgtctgca gactatagcg cttgagttcg
     1261 gtcccatcat cgacacactg gattatgatg gcgatgggaa ggcaggagat ctggcctatg
     1321 tcgtcgctag cggcggtcct ggaaagatag gcctcgattc ctttgagcgg taccacaaca
     1381 tgctcacgtt tcgcttcgac tctcccatct gcgccggagg cccccatagc gagggcgata
     1441 gtacctactt ctttggcctt gtctccgcac ggccgtcgag attcgtcaca gcaaccatca
     1501 aggagacgac cggtctaagc tctgtgtcgc caaaaatgaa gaaaaatcta cggcacaaag
     1561 tgcaggtccg tgcacctcag ataggcacgg cggagtgagc cggatcgccc cggaagtctt
     1621 tgagatcgtt tctgggattg tcagagtagc ctgtggcgat ctggcggata tgatccgaat
     1681 catgccggcc aaaggacgga atagagagcc aggacggact agcttgagct taatgaccat
     1741 agtcgatctt cgaccaggaa gtcttttcca gacagcgctc atcttcaccg cgatccttta
     1801 caggggaggg ccgcctcggc tatagtcggg gtatggccac cacacctgaa tcttctcgct
     1861 gaaatcgccc tgccggtccc gccacgtaag gtcctgacgt ataccatccc gcccgcccta
     1921 cagagccagg ctgaggtcgg caagcgagcc ctcgtcccgc tcggcccacg catggtgacc
     1981 gggtatattg ttggtctcca cacgttcagc gatcaaccgt cagccgtcag gcatcaggca
     2041 tcagagctca agccgatcga cacgatcctg gacccggagc cattgctcga ccaccacatg
     2101 ctggagctaa cacgccgcgt agcggactac tatctgacgt cgtgggggct ggttattcgg
     2161 gcagccctgc cccccgggat cgatcgtagg acggctcgaa ctgtcgagct catcgagtct
     2221 ccagacctgt tcccacatgc gttaaacggg gagcgcctcg cccaaaaagc cggcaagctt
     2281 ggccccatcc agcagcagat gcttgcctct ctgcaagcgc aacgccggat gctgctttca
     2341 tccctcaaac agcggtggcc gaacgaagag gtcgatcggc tcatccgcat gcttgtcaga
     2401 caaaacctgg cgaaggtgga ataccgcgaa tgtctaccgt tgattcggcc tgcctatcgg
     2461 tccttgctca gtctagccgt cgaccaggcc tctgcggagg ccgaattggc cgctctccga
     2521 caccgtgcgc cgcgacaagc ctcgttgctc gagcgcctgc tgcaatccga ctcaaagctc
     2581 acatctgcgg aggccacagt cctcgccggc gcctccgggg ttcgtgccct catcgccaaa
     2641 ggcttcatcc atcgagtcac ggaggaggtt gagcggtctc cctgggaaga gaccactgct
     2701 gccactggct cctggcccga gcctaactca gcccaacaaa tagcgattga cgggctcctg
     2761 gagggactac cctctcacac atttgtcccg gcgctactcc atggcgcaac ggggagcggg
     2821 aaaaccgagg tttatcttcg agtgatcggc gaggtggtac ggcagggaag gcaggcgctc
     2881 gtgctcgttc cggaaatcgc cctgacccct gttactgccg atcgattccg ctcccgcttc
     2941 ggggatcggg tcgcactgct gcacagcggc ctctcgcctg gcgagcggct ggatcagtgg
     3001 caccgcatca agcgcgggat ggccgatatc gtcg
//
LOCUS       DATMXI010000089         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_11512, whole genome shotgun sequence.
ACCESSION   DATMXI010000089 DATMXI010000000
VERSION     DATMXI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11512"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VN444_01675"
     CDS             complement(<1..637)
                     /locus_tag="VN444_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HWR20550.1"
                     /translation="MSEQDIEVATRLNVFERYLSIWVALCIVTGVAIGKVSPGLVGTL
                     SRMEVSHVNLPVAVLIWLMIYPMMLRIDFSAIRGVGRRPKGILIVLFVNWLVKPFSMA
                     FLGWLFFKHLFAGIIGPSLGDQYLAGTIILAAAPCTAMVFVWSYLTDGDPAYTLVQVA
                     LNDLIMLFAFAPIVICLLGVSNITVPYDVLLYSVLLYIVIPLAAGAASRTTL"
     gene            complement(693..1019)
                     /locus_tag="VN444_01680"
     CDS             complement(693..1019)
                     /locus_tag="VN444_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWR20551.1"
                     /translation="MPSLISKGVEQAVRRFHALADETRLQIIERLCDGEQCVCDLSGM
                     LGTAQSLLSFHLKTLKDAGILRDRREGRWVYYSLNPEAIAELADLIGSIKPRGRGLLV
                     ASNRCK"
     gene            complement(1294..>2536)
                     /locus_tag="VN444_01685"
     CDS             complement(1294..>2536)
                     /locus_tag="VN444_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20552.1"
                     /translation="MADGDFSCKISALSSDEIGQLGGALNLMTRRLEDRLAALEEERA
                     KGAAILDSMVEGVLAVDGESRILLINAGACRLFNVSSVGTEGKPFLEVIRNKEMLDLL
                     NHTLREGVFAKQELQIFTPVQRVLQVHASPLKGRERTSGTMLVMHDVTELRRLETIRT
                     EFVANVSHELRTPLTSIRGYLETLLEGGLEDRAHARPFLDVIHKHTERLGRLLDDLLD
                     LSNLELGKVTLHRQPTVLAEVVHNVMTIYGPQAAKQEIELRAELPHDLPHVLADRDRL
                     VQILINLLDNGLKFTPKGGRVTVTAKMVRGSRFEGHGSESEIRNPKSETPSLSEAVEV
                     SVQDTGIGIPSQDLPRITERFYRVDRARSREMGGTGLGLAIVKHLVKAHGGELVIESQ
                     LNQGTTVRFTLPIAVADPEAS"
BASE COUNT          536 a          749 c          742 g          509 t
ORIGIN      
        1 ggagtgtcgt ccgagaggct gctccggctg caagagggat gacgatgtag agtaggaccg
       61 agtagagcag cacgtcgtac ggtaccgtga tgttcgagac gcccaggagg cagatcacga
      121 tgggcgcaaa ggcgaagagc ataataaggt cgttcagcgc cacctggacc agcgtgtagg
      181 ctgggtcgcc gtcagtcagg tagctccaga cgaagaccat ggccgtgcac ggggcagccg
      241 ccaggatgat ggtcccggcg aggtactgat ctccgagcga gggaccgata atcccggcaa
      301 agaggtgctt gaagaagagc cagccgagga aggccatgct aaaaggcttt accagccagt
      361 tcacgaagag gacgatcagg attcccttcg gtcgccgccc gacacccctg atggcagaga
      421 agtcgatccg cagcatcatc gggtagatca tcagccagat caggaccgcg acagggaggt
      481 tgacgtgact cacctccatc cggcttaagg tacccaccag tccggggctg accttaccga
      541 tggcgacgcc cgtcacgata cagagcgcca cccagataga gaggtagcgc tcgaagacgt
      601 tcagccgcgt tgcaacttcg atgtcctgct cactcatgtt ggcctcccgc tatccaaatc
      661 aagattcatt gatacatgag cgccgaaaaa agtcacttgc agcggttcga tgccacgagc
      721 aagccacgac ccctcggctt gattgaaccg atgaggtctg ccaactccgc aatcgcctct
      781 ggattcagtg agtagtagac ccaccgcccc tcccgtcggt cacgtagaat tccggcgtcc
      841 ttgagcgtct tcagatgaaa ggaaaggagc gactgggccg ttccgagcat gccgctgagg
      901 tcacagacgc actgctcgcc gtcacacaag cgctcgatta tctgtaagcg cgtctcatcg
      961 gccagcgcat ggaaccggcg caccgcctgc tcgacacctt tcgaaatcag agatggcata
     1021 cggctaatat atcaagatct attgatttgt caaattcctg gatttgtcga gcatgatgaa
     1081 ctaccccgag acaatctgcg gggtatcgtc ttctgttctg gcccgtcatt ccgtgcttga
     1141 cacggaatcc agtcgggccc tctggatacc ggcttccgcc ggtatgacga actcgcggca
     1201 cgccgcgggg aatgaacccc cagtggattc aagatggcaa cacagaactt tcgggtctga
     1261 ttggcagcag gcaaacgagc ggctttggct cagtcacgag gcctcagggt cggctacggc
     1321 gatcgggagc gtgaagcgga cggtggtccc ctggtttaat tggctttcga tgactagctc
     1381 tccgccgtgc gccttgacca ggtgcttcac aatggccaag ccaagtcctg tcccgcccat
     1441 ctctcgagag cgggctctgt ccacccggta gaaccgctcc gtgatcctgg gcaagtcctg
     1501 ggaggggatg ccgatgccgg tatcctggac ggagacttcg accgcctcgc tgaggctcgg
     1561 tgtttcggat ttcggatttc ggatttcgga ttctgaaccg tgcccctcga accttgaacc
     1621 tcgaaccatc ttcgcagtga ccgttactcg cccgcccttt ggtgtgaact tgaggccgtt
     1681 gtccaggaga ttgatcagga tctgcaccag acggtcgcgg tcggccagaa cgtgcggcag
     1741 atcatgggga agctcggccc gaagctcgat ctcctgcttg gctgcctgcg gtccatagat
     1801 ggtcatgaca ttgtgtacta cctcggcaag gactgtcggt tgccgatgaa gcgtgacctt
     1861 tcccaattcg aggttggaga ggtccagcag gtcgtccagg agccggccga gtcgttcggt
     1921 atgcttatgg atcacatcga gaaacggacg ggcatgcgcc cggtcctcca gcccgccttc
     1981 cagtagtgtc tccaaatatc ctctgatgga ggtgaggggg gtgcgaagtt catgagagac
     2041 gttggccaca aactccgtcc gaatcgtctc gagacggcgc aactcggtga catcgtgcat
     2101 caccagcatt gtgcctgatg tccgctcccg gcccttgagg ggagaggcgt gcacttgaag
     2161 aactcgctgt acgggagtaa agatctgaag ctcctgcttg gcgaagaccc cctccctgag
     2221 cgtgtgattc aagagatcta acatctcctt gttgcggatc acctcgagaa acggctttcc
     2281 ctccgtcccg actgacgaga cgttaaagag ccggcaggcg ccggcattga tcaacaggat
     2341 ccggctctcc ccatcaaccg ccagcacccc ttccaccatg ctgtcaagga tggcagcccc
     2401 tttagcccgt tcctcttcca gcgcggcgag ccggtcctcc agccttctgg tcatcagatt
     2461 cagggcgccc cctaactggc cgatttcatc ggaggagagc gcggaaatct tgcaggagaa
     2521 gtccccatcg gccatg
//
LOCUS       DATMXI010000090         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_306296, whole genome shotgun sequence.
ACCESSION   DATMXI010000090 DATMXI010000000
VERSION     DATMXI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306296"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VN444_01690"
     CDS             complement(<1..532)
                     /locus_tag="VN444_01690"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HWR20553.1"
                     /translation="MQLGAHEKREAMDRNLALELVRATEMAAISSARWMGLGNPSAAE
                     QAAIDAMRHAFDSVSFCGSIVIGKGERGKAPTLYVGEVFGQGDAPEVDVALDAVESGT
                     IVANGRQNAISVIAAAEKGSLRHIPDAHMEKIAVGPKAAGAIDITAPPEKNLRAIAEA
                     MNCSVEDLTVVILDRPR"
     gene            complement(654..1007)
                     /locus_tag="VN444_01695"
     CDS             complement(654..1007)
                     /locus_tag="VN444_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWR20554.1"
                     /translation="MACALPPRGGHDASVMVLVRQEGEAASFRAHLQPKASREAIVGE
                     ADGVLRLRVTAPPIDGRANEACLRLLAKALDLPVSRLRIAGGQHARIKTIRIADTSAG
                     LLRTALCNLLEHPKR"
     gene            complement(962..1261)
                     /locus_tag="VN444_01700"
     CDS             complement(962..1261)
                     /locus_tag="VN444_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWR20555.1"
                     /translation="MPFVAHFIDAFASILGQVLGLYTWVLIIRAVISWVSPDPWNPIV
                     QFLCRVTDPVLQPIQRLLPPWRLGIDISPIVAILAIQFIQQWLVPSLREVAMMLQ"
     gene            complement(1267..2082)
                     /gene="proC"
                     /locus_tag="VN444_01705"
     CDS             complement(1267..2082)
                     /gene="proC"
                     /locus_tag="VN444_01705"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWR20556.1"
                     /translation="MLQGRTIGFIGAGNMAEALIRGLLEARLVTADQLIASDIVEAKR
                     QQIHQRHGIQTVTEGRDVAMKASILVLAVKPQDMETALKGIAASVDQAKTIISVAAGI
                     TIAFIAERLPAKTRIIRAMPNAPALVLAGAAGIAKGEHATAEDLQCAEAIFAAVGKAV
                     VVEETHLDAVTGLSGSGPAYAFLFIEALADAGVKMGLARDVARLLAAQTVLGAAKMVL
                     ESGRHPAELKDMVASPGGTTIAGLHVLERGGLRGTLIEAVEAATIRSRELGRR"
BASE COUNT          448 a          697 c          671 g          409 t
ORIGIN      
        1 gacgaggtcg gtcaaggatg accaccgtga gatcttctac agaacagttc atcgcttcag
       61 cgatggcgcg gagattcttc tctggggggg cagtgatgtc aatcgccccg gccgccttag
      121 gcccgacggc gatcttttcc atgtgggcat ccggaatatg gcggagcgat cccttctcag
      181 cggcggcaat gacggagatg gcattttggc ggccgttggc cacgattgtc ccgctttcaa
      241 cggcgtccag cgccacgtct acttcagggg cgtcaccctg gccaaagacc tctccgacat
      301 agagggttgg cgccttgccg cgctcaccct tgccgatgac gatggaacca caaaagctga
      361 cgctatcgaa ggcgtggcgc atggcgtcaa tcgccgcctg ttcagccgcg ctcggattgc
      421 ctaaccccat ccagcgagct gacgaaatag cggccatctc agtcgcccgc actaactcaa
      481 gcgccagatt ccggtccatc gcctctctct tctcatgagc cccgagctgc aagcggctca
      541 ctcctgttcg ctgtacgctc cacacccata aaatcgatag aagtatagga aatacgcggg
      601 atcgagtcaa gcccaacttc tgcagcttca cggaccctca gacagagtgg gggctagcgc
      661 tttggatgct ccagcagatt gcagagtgca gtacggagaa gaccggcgga ggtgtcggca
      721 atccggatag tttttatgcg cgcgtgctga ccaccggcaa ttcgaaggcg ggagaccggc
      781 agatcaaggg ccttggcaag caggcggagg caggcctcgt tggcccgacc atctatcggg
      841 ggggcggtta cccgaagccg cagaacaccg tcggcttcgc cgacaatcgc ttcgcgggac
      901 gccttcggct gaagatgggc acgaaaggag gcggcctcgc cctcctgtcg aaccagaacc
      961 attactgaag catcatggcc acctcgcgga gggagggcac aagccattgc tggatgaact
     1021 ggatcgccag aatagcgacg atgggagaga tatcgatccc caatcgccac ggtggcagta
     1081 atctctgaat cggctgtagt accgggtcgg taacacggca gaggaattga acgatcgggt
     1141 tccaggggtc ggggctgacc caggagatga ccgcccggat gatgagaacc caggtgtaga
     1201 gccccaacac ctggcccaga atggacgcaa atgcatcaat gaagtgggcg acgaacggca
     1261 tagtgattat ctcctgccca gttcccggga tcgaatcgtg gcggcctcta ccgcctctat
     1321 cagggtcccg cgcaatccgc cgcgctccag aacatgcagc ccggcaatag tcgtcccacc
     1381 gggggaggcg accatgtcct tcagttcggc cggatgacga ccgctctcga gcaccatctt
     1441 tgccgcgccc aggacggttt gagcggccag cagtctggcg acatcccgcg caagccccat
     1501 cttcacaccg gcgtcagcca gcgcctcgat aaacaggaac gcgtaggctg ggccgctgcc
     1561 gctcagtccc gtcacggcgt ccaggtgtgt ctcctcaacc acgaccgctt tcccgaccgc
     1621 ggcgaagatc gcctctgcac actgcaggtc ttcggctgtc gcgtgctcac ccttcgcaat
     1681 gccggcggca ccggccagga ccagggccgg cgcattgggc atggcgcgaa tgatccgcgt
     1741 cttggcgggc aggcgctcgg cgatgaaggc gatcgtgatg ccggcagcaa ccgagatgat
     1801 cgtcttggcc tgatccacag aagcggcgat ccctttcagc gccgtctcca tgtcctgggg
     1861 cttgacggcc aggaccagga tcgaggcctt catggccacg tcgcgaccct cggtcaccgt
     1921 ctggatgccg tggcgctggt ggatctgctg tcgtttcgcc tcgacgatgt cagaagcgat
     1981 aagctgatcc gccgtcacca gcctggcttc aagcaggccg cggattaacg cctcggccat
     2041 gttgccggcc ccgatgaacc cgatcgttcg accttgtaac atgccgcgct ccttacgtcc
     2101 aaaaggcctc tcgacttata gcgccaaacc ccaggcgagt caaggagatt tgcctatcaa
     2161 ctactccttg cggtcgtcga gaaattacgg tatatggtgg ggaccgccga tgctggatgc
     2221 ggaag
//
LOCUS       DATMXI010000091         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_258241, whole genome shotgun sequence.
ACCESSION   DATMXI010000091 DATMXI010000000
VERSION     DATMXI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258241"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2572
                     /gene="fdhF"
                     /locus_tag="VN444_01710"
     CDS             <1..>2572
                     /gene="fdhF"
                     /locus_tag="VN444_01710"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWR20557.1"
                     /translation="CKDPDRPALGACRTCLVKVEGMRGFPASCHLPASEGMVVSTNDP
                     VLTRIRNGVLDLTCGMATPSFDRPGFGQLSDACARLGLFHRRHMPWRHTPVDDSKSFF
                     ILDRDACILCGRCTVACDDVQQIGAIALLGRGGSTTVGVFGDGSLASSVCTSCGQCVA
                     TCPTGAIRPKEAPAPIVREVETTCPYCGVGCGITLRVRADGRLALMADDVPTNRSSLG
                     MLCVKGRFGTGFVHAPDRITRPMVRDGGRWREVSWDTALDAAAEGLVRNRGRFGVLAS
                     AKATNEDGYIAQKFSRVVMGTNNIDHCARLCHSPSVVAMLASLGSGATSNSYTDFEEA
                     GCLMVVGSDPSSNHPVIAVRLRRTVGRGARLIVVNPKRIELCDQADLWLRPRPGTDVT
                     LFNAIARVIVDEGLANLQFVQSRTEGFEAWRQSLEPYTLEFAERMTAVPREQIRQAAR
                     WYAKPPFAGSCLLWGMGVTQHTNGTANAHALLNLSLVTGQMGFAGSGISPLRGQNNVQ
                     GCGDAGCLPTHLPGYQRYTPEALEKFEAAWGVRPPSSEGMKLTEMVDGCLDGTIRAMY
                     MVGENPLLTEPDLHHAKKALSNLDCLVVQELFLHETAELAHVFLPTAAFAEKEGTFTN
                     SERRVQRVRKALDPPGEARPDWWITCELARRVAQRLSVPSSGFGHSHPAEIFDEMARM
                     VSFLGGISHDRLDREGGIQWPCPTSDHPGTRFLYAESFPVGKAKFIPVVQTAEAAELP
                     DHDFPFLLNTGRILYHWHGGTLTRRVQGLLELAPRLEVAINPDDARRLGLEQGALVRL
                     TSRRGELTAYAQITEAVRAGEVFIPFVKLAESGANILTNSVYDPAAKIPEYKVCAVKI
                     E"
BASE COUNT          458 a          841 c          794 g          479 t
ORIGIN      
        1 tgcaaggatc cggaccggcc tgcccttggg gcctgccgga cctgcctggt caaggtcgaa
       61 gggatgcggg gcttcccggc ttcctgtcat ctcccagcca gcgaggggat ggttgtctcg
      121 accaacgatc ccgttcttac acggatccgg aacggagtcc ttgacctcac gtgcggtatg
      181 gcgaccccct cgtttgatcg gccgggattc ggtcagctct ccgatgcctg cgccagactt
      241 gggctcttcc accgccgcca tatgccatgg cgacacacgc ctgtggacga tagcaaatcg
      301 ttcttcatcc tcgatcggga cgcctgcatc ctgtgtggcc gctgcaccgt agcctgcgac
      361 gacgtccagc agatcggcgc tatcgccttg ttggggcgag gcggctcgac tacggtggga
      421 gtcttcggcg acggatcgct cgcttcctcc gtctgcacct catgtgggca gtgcgtcgcc
      481 acctgtccca cgggggccat ccggccgaag gaagcgcccg cgcccatcgt gcgtgaggtt
      541 gagacaacct gcccctactg cggcgttggt tgcggcataa cccttcgcgt tcgcgccgac
      601 gggcgcctgg cgctgatggc cgacgatgtg cccacgaatc gatcgagtct cggtatgctc
      661 tgcgtgaagg gacgattcgg gaccggattc gtccatgctc ccgaccggat cactcgtccc
      721 atggtgcggg atggcgggcg ctggcgggag gtctcgtggg atacggcgct cgacgccgcg
      781 gcggagggct tggtgagaaa tcgaggacgc ttcggcgtgc tggcctcggc aaaggccacc
      841 aacgaggacg gttacatcgc tcagaaattc tctcgggtgg tgatgggcac caacaacatc
      901 gaccactgcg cccgcctctg ccattcgccc tcggtggtgg caatgctcgc ctcgttgggc
      961 tctggggcca cctccaactc ctacaccgat ttcgaggagg ccggctgcct catggtcgtc
     1021 ggctccgatc cgtcatcgaa ccatccggtg atcgccgtcc gtctccgccg caccgtgggc
     1081 cgtggcgcca ggctcatcgt cgtgaacccg aagcggatcg agctgtgcga ccaggcggac
     1141 ctgtggctgc gcccgcgacc gggtaccgat gtgacgctct tcaacgccat agcgagggtg
     1201 atcgtggacg aaggactggc aaatcttcag ttcgtgcaaa gccgcaccga agggttcgag
     1261 gcgtggcgac aatctttgga accgtatacc cttgagttcg ccgaacggat gacggccgtc
     1321 ccacgcgagc agatcagaca ggcagcccgc tggtacgcaa agcccccctt cgccggctcc
     1381 tgcctgctct gggggatggg ggtgacgcag cacacgaacg gaaccgccaa cgcccatgcc
     1441 ctcctgaacc tctcccttgt caccggacag atgggttttg ccggctctgg catctcaccg
     1501 ctccggggcc aaaataacgt ccaggggtgc ggggacgccg ggtgtctccc tacccatctg
     1561 cccggctatc agcgctacac ccccgaagcc ttagaaaagt tcgaggcggc ctggggcgtt
     1621 cgacctccca gcagcgaagg catgaagctc actgaaatgg tggatggatg cttggatgga
     1681 acgatccggg ccatgtacat ggtcggcgaa aaccccctcc ttacagaacc cgatcttcat
     1741 cacgcgaaga aggccctttc caacctcgac tgcctcgtag tccaggagct gttcctgcac
     1801 gagacggcag aactggccca cgtcttcctc cccacggccg cctttgccga aaaagagggg
     1861 acgttcacca actcggagcg gcgagtccag cgcgtgagaa aggcgctcga tccaccggga
     1921 gaggccaggc cggactggtg gattacctgc gaattggcgc gacgggtcgc ccagaggctt
     1981 agtgtaccga gttctgggtt tgggcactcc cacccggcag agatcttcga cgagatggcg
     2041 aggatggtct cgtttcttgg tgggatctca cacgaccgac tggaccgcga gggcggtatc
     2101 cagtggccct gcccgacatc agatcatccg ggcacccgct tcctgtatgc cgagtcattt
     2161 cctgtcggga aggcgaaatt cattccggta gttcaaacgg ccgaggcggc cgaactaccc
     2221 gatcacgatt ttccgttcct cttgaatacc ggtcgcatcc tgtaccactg gcacggcggg
     2281 acactcaccc ggcgggtcca ggggctcctc gaactggcac cacggctcga ggtcgctatc
     2341 aacccggacg atgcccggcg acttggcctc gaacaggggg cgctcgttcg actcacctcc
     2401 cgtcgcggcg agctgaccgc atacgcccag atcaccgagg ccgtgcgagc aggagaggtc
     2461 ttcatcccct tcgtgaagct tgccgagtcg ggagccaata tcctgaccaa ctcggtctac
     2521 gatcctgccg ccaagatccc ggaatacaaa gtctgcgccg tcaaaatcga ac
//
LOCUS       DATMXI010000092         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_258225, whole genome shotgun sequence.
ACCESSION   DATMXI010000092 DATMXI010000000
VERSION     DATMXI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258225"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..779
                     /locus_tag="VN444_01715"
     CDS             447..779
                     /locus_tag="VN444_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HWR20558.1"
                     /translation="MNTEIIAGHGTVEARKEVLVKDLKSVVGDADALLKEVASSTAEG
                     FSAARTNVERRLGEAKSRLVYARLAVTEKARHAAGATNEYVKENPWRVLGVAAASGLI
                     IGFLLSRR"
     gene            905..>3374
                     /locus_tag="VN444_01720"
     CDS             905..>3374
                     /locus_tag="VN444_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWR20559.1"
                     /translation="MNEALISVLLVEDDLADAELIKGALAGMGDSPFRVDWVTSLSDA
                     LERLGREGIDVVMLDLTLPDSQGIEAFDKILQAAPNALILVLSGATDEETARQAVQRG
                     AHDYFTKGHVDAHWLPRALRYIIERKAIQHALKNSEVRFRSMSDASPLGIFVSDEQGS
                     CVYTNAAYHKISGLTFEQTLGTNWSMAIHPEDRERVLAEWRIAARSQDPFQTEYRFQQ
                     EDEGVVWARVNSAAMNDGMKSFGLVQTVEDITDRKVAEFELTAAQEALFVEKERAQVT
                     LNSIGDAVLTTDLLGTVTYMNLVAEAMTGWSRERALGRPLSEVFKIIDGTTRQAAANP
                     AERAIEEDRTVGLAADSVLVSCDGLEFAIEDSAAPIHNRDGRVAGAVIVFHDVSETRA
                     MALKMSYLAQHDFLTGLPNRALLTERLSQSIGLAQRHRKQVALLFLDLDFFKLINDSL
                     GHAIGDQLLQSISHRLTACVRATDTVCRQGGDEFVILLAEIERPQDAANVAEKMLAAL
                     AEPHHIGENEIHVTLSIGISVYPDEGTNAETVMQNADTAMYHAKAKGRNNYQFFRAEM
                     NTHAVRRLFVETSLRRALKLGEFLLHYQPKIDIASGEMTGCEALIRWQDPDLGLIYPE
                     QFVPIAEECGLIVPIGRWVLREACRQVQTWLDSGLCAVPVAVNISAVEFKHKDFLEGV
                     ALILKETGLAPHYIEIEITESILMHEAESSTSVLEALKDIGVKLAIDDFGTGYSSLSY
                     LKRFPIDTLKIDQSFVRDIATDADDAAIVGAVIEMGKNLKQRVIAEGVETHEQFAFLQ
                     ARRCEEGQGFHFSHPLCAEDFTPLL"
BASE COUNT          756 a          898 c         1004 g          716 t
ORIGIN      
        1 gtccagcgtt gcgaaaacgt gcccagccag gctcgccccg actattggga tgtcacctac
       61 aacttccggg gccaggagta tcgggtccag atgaccgctc cccccggcaa cacggttact
      121 gtcaacgagc agggggaacc ccgggtataa cgattgacgc agtggattga tcgaaacggc
      181 ctcgcggggc cgtttcgctt tgtgctgcgc ccgtaaggtt gtgcacacgc ttgggagcgg
      241 cagactctgg gtgcggtggc gtacagactg ccggcctggc atggatcagc atgcgatcta
      301 cggggcggat tcgctcgaaa gcctgactgc ttgtcatgat tgactggcgt gtcaaatgaa
      361 aaagcgattc tgttcctctc tgatttactg aacagatgtg acgcgagcgc tttgcgctat
      421 ttttaactta tgctccagga gacacgatga atactgaaat aatcgcaggc catggcactg
      481 tcgaagccag gaaggaagtc cttgtgaagg atctcaagag cgttgtcggc gatgccgacg
      541 ccctgcttaa ggaggtggct agctcaacag ccgagggatt ctctgcggcg cgcaccaatg
      601 tcgaaagaag gctgggtgag gcgaaatcca gactcgtcta tgcgcgactc gcggtcactg
      661 aaaaggcccg acacgctgca ggcgccacca atgaatacgt aaaggagaat ccctggaggg
      721 tgctcggggt tgcggcagct tcagggctta tcatcggttt tcttttaagc cgccgctgat
      781 caggtgtttt ctagaaatag cctgtattca agacgatcaa tagacgcctc atagctgttg
      841 attggctcag gcatagtcaa ctatccgttg tattcaatcg atccccattg cgaacgacta
      901 tgccatgaat gaagccctga tctctgtgct tctcgtcgag gacgatctcg ccgatgccga
      961 gttgatcaag ggcgcactcg caggtatggg agatagtccg tttcgtgtcg attgggtaac
     1021 gagtctttcc gatgcgctcg aacgactggg cagggaaggt atcgatgtgg ttatgcttga
     1081 ccttacgctt cccgacagcc aaggtatcga ggccttcgat aagatattgc aggccgcacc
     1141 gaatgctctg atcctggttc tgagtggtgc gaccgatgag gagaccgccc gccaggctgt
     1201 gcaacgcggc gcgcatgact atttcacaaa aggccatgtc gacgcccatt ggttgccgcg
     1261 cgcactgcgc tatatcatcg agcgaaaggc tatccagcac gcactaaaga acagcgaggt
     1321 gcgctttcgc tcgatgagtg atgcttcccc gctgggcatt ttcgtttccg atgaacaggg
     1381 gagttgcgta tataccaacg cggcctatca caagatatcg gggctgacat tcgaacagac
     1441 cctgggcacc aactggagca tggcgatcca cccggaagat cgcgagcgtg tcctggccga
     1501 gtggcgcatc gcagcgcgga gccaggatcc attccagacc gaatatcgct ttcagcagga
     1561 agacgaggga gtcgtgtggg cgcgcgtcaa tagtgccgcc atgaacgacg gaatgaaatc
     1621 gttcggtctt gtgcagacgg ttgaggatat caccgaccgc aaagtggcgg agttcgagtt
     1681 gacggcggca caagaggcct tattcgtgga aaaggagcgc gcccaggtca cgctcaactc
     1741 cattggcgac gccgtactga ctaccgacct tcttggcacc gtgacttata tgaatctggt
     1801 ggcggaggca atgacaggct ggtctcgcga gcgcgcgttg ggccggcctc tttctgaggt
     1861 tttcaagatc atcgacggca cgacacgtca ggccgctgcg aatcctgcgg agcgcgccat
     1921 tgaagaagac aggaccgtag gattggctgc ggatagtgtg cttgtcagct gcgacggtct
     1981 cgagttcgcg atcgaggatt ccgccgctcc catccacaac cgcgacggtc gggtcgccgg
     2041 cgcagtgatc gtcttccacg acgtcagcga aacgcgggcc atggcactga agatgtccta
     2101 tctggcccag cacgactttc tcacaggatt acccaatcgg gcgcttctga cagaaaggct
     2161 ctcacagtca atcgggttgg cgcaacggca tcgcaagcag gttgcgctgc tttttctgga
     2221 cctggatttt ttcaaactaa tcaatgactc actaggccat gccatagggg accagttgct
     2281 gcagtcgata agtcaccgct tgacggcgtg tgttcgcgca acagatacgg tctgccgcca
     2341 gggaggtgat gaatttgtga tactgcttgc cgaaatagag aggccacaag atgcggccaa
     2401 cgttgcagaa aagatgctcg ctgcgctcgc tgagccgcat cacatcggcg aaaacgaaat
     2461 ccatgtcact ttgagcatcg gtatcagcgt ctatccggat gaaggcacaa atgctgagac
     2521 cgtgatgcag aacgcggaca ccgcgatgta ccacgcgaag gcaaaagggc gcaacaacta
     2581 ccagttcttc agggccgaga tgaacacgca cgcggttcgc cgtctgttcg ttgaaaccag
     2641 ccttcgccgc gccctgaagc tgggggagtt cctgctgcat taccagccga agatcgatat
     2701 tgcttcaggg gagatgacag gatgcgaggc gctgattcgc tggcaggatc cggatctcgg
     2761 gctcatctat ccggagcagt ttgtaccgat tgcggaggag tgcggcctta tcgtgccgat
     2821 agggcgatgg gtgctgcgtg aagcctgcag gcaagtccag acctggctgg attcgggact
     2881 gtgcgccgtg ccagtggcag tcaacatatc tgcagtggag ttcaagcaca aggatttcct
     2941 cgaaggcgtt gccttgatcc ttaaggagac cggcctggca ccgcactata tcgagatcga
     3001 gatcacagaa agcatcctca tgcacgaagc cgaatcatcg acttcggtgc ttgaagcact
     3061 caaggatatt ggtgtgaaac tggccatcga tgacttcggt acaggctatt cgagtctgag
     3121 ttatctgaaa cgtttcccaa tcgataccct gaagatcgac caatcctttg tacgcgacat
     3181 cgctacagat gccgacgacg cagccatcgt tggcgcggta atcgagatgg ggaagaacct
     3241 caagcagcgg gtcattgcgg aaggcgtgga aacccacgag cagttcgcct tcctgcaggc
     3301 acggcgatgc gaggagggac agggatttca tttcagtcac cctttgtgcg ctgaggactt
     3361 cactccattg ctcg
//
LOCUS       DATMXI010000093        10520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_103083, whole genome shotgun sequence.
ACCESSION   DATMXI010000093 DATMXI010000000
VERSION     DATMXI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103083"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VN444_01725"
     CDS             complement(<1..136)
                     /locus_tag="VN444_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWR20560.1"
                     /translation="MGKAKFERTKEHMNIGTIGHIDHGKTTLTAAITKVLHAANAKVA
                     F"
     gene            complement(170..2263)
                     /gene="fusA"
                     /locus_tag="VN444_01730"
     CDS             complement(170..2263)
                     /gene="fusA"
                     /locus_tag="VN444_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWR20561.1"
                     /translation="MPDIYPIETVRNIGIMAHIDAGKTTTTERILYYTGRTYKIGEVH
                     DGSTEMDWMEQERERGITITSATTTCFWRDHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VALFDAVAGVEPQSETVWHQADKYGVPRIAFINKMDRMGANFDMCVRMIAERLGAVPL
                     VVQLPIGREDHFEGVVDLIRMKAVIWDDETLGASYREDEISEEMSSLAQTARERMLET
                     IAEVDDSFLVRYVDGLAVGPEEIKSAIRSAALKLLLVPVLAGASFKNKGVQLLLDAVV
                     DYLPSPVDLPPIEGLDSTRTKSVVRVPKVNDPFAALVFKIMTDPYVGQLAFFRVYSGT
                     LASGSHVYNSTRGTRERIGRLLQMHANKREEIKEVFAGDIAAAVGLKGAKTGDTLCDE
                     GNQIILESIVFPEPVISVAIEPKTKEGQDRLATGLQALANEDPTFRASTDEETGQTII
                     SGMGELHLEIIVDRLTREFRVEANVGKPEVAYRETVTITAEAEGKYVRQTGGRGQYGH
                     VWLKVEPSTAQSGFEFVNKIVGGVVPKEYISAVEKGIKEALHTGVVAGYPVIDLKVTL
                     FDGSYHEVDSSEMSFKIAGSMAFKEATKRAKPVLLEPIMRVDVSTPEAFLGEVIGNLN
                     SRRGRVIGIEVRSVAQVIQADVPLSEMFGYATDLRSATQGRAAHSMQFSRYEPVPASI
                     AGEIVARMGGRHGGR"
     gene            complement(2332..2802)
                     /gene="rpsG"
                     /locus_tag="VN444_01735"
     CDS             complement(2332..2802)
                     /gene="rpsG"
                     /locus_tag="VN444_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWR20562.1"
                     /translation="MPRRKVIEKRETLADPVYSNRMVTKFINSMMVQGKKSVAESIVY
                     GSMKIIEDRAKTDPLRMFKQAVDNVKPVLEVKSRRVGGATYQVPVEVRAERRNSLAFR
                     WIIGFSRKRTEKTMAERLAAELIEAAANRGSSVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(2908..3285)
                     /gene="rpsL"
                     /locus_tag="VN444_01740"
     CDS             complement(2908..3285)
                     /gene="rpsL"
                     /locus_tag="VN444_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWR20563.1"
                     /translation="MPTIHQLVRKGRSAAVKKAKTPALQGCPQRRGVCIRVYTTTPKK
                     PNSALRKVTRVRLSNGIEVTTYIPGVGHNLQEHSIILIRGGRVKDLPGVRYHVIRGAL
                     DTAGVQDRKQGRSLYGAKRPKIS"
     gene            complement(3361..7458)
                     /gene="rpoC"
                     /locus_tag="VN444_01745"
     CDS             complement(3361..7458)
                     /gene="rpoC"
                     /locus_tag="VN444_01745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWR20564.1"
                     /translation="MDKFFGFYDEKTIDPTSFKAIRIGLASPEKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCSKIFGPTKDWECNCGKYKGIKHKGVVCDKCGVEVIRSKARRQR
                     LGHIELVSPVVHVWFYKGVPSRIGYLLDISLRDLERVLYFEAYVVLNPGKTPLSLKQL
                     LTEEQHRLAYEEYGDGFKAGMGAGAIRDLLKRLNLRELAEELHAQMPQETSQQGRKKI
                     TKRLRIVEAFINSSNRPEWMILDAIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLRRLIELRAPDIIVRNEKRMLQEAVDALFDNGRRGRALKGQNNRPLKSLSEMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIFRKLLKDGVV
                     TTIKSAKKLVEKGKPEVWDALEEVIHEHPVLLNRAPTLHRLGVQAFEPVLVEGEAIKI
                     HPLVCAAFNADFDGDQMAVHVPLSPEAQIEASVLMLASHNILSPANGLPVVTPSQDIV
                     LGCYYLTRSRAGEKGEGYVFSDMDEVRAAYDAEEVALLARIKVRVEGEMIETTVGRCI
                     FNEQLPETLRFVNQEMNRRELTRLVAQCCYLLGNTVTVKLLDNLKDLGFRYATLAGIS
                     IGIDDMHIPSTKGKVIDDANKEVVKVEREYQDGLITKGERYNKIIDIWTHVTERVSDE
                     MFREIEAEEKGEFNPIFMMADSGARGSRQQIRQLAGMRGLMAKPSGEIIETPITANFR
                     EGLTVLQYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVAEVDCGTPNGIWV
                     TPLIEGGEVIQPLRDRILGRVALDEILDPFTGEVLVGANQEIVEVLASMIENAGLDRV
                     KIRSVLTCEARRGICIRCYGRNLATGRLVELGEACGVLAAQSIGEPGTQLTMRTFHIG
                     GTASRSVEQSTLECRGAGIVRFAGLRTVKAPQGDYVVMNRNGAISIIDEKGRKKENYP
                     AVYGAHMKVADGATVKAGQVLMEWDPFTNAILTEHGGKVHFRDVLEGVTIREEVDEVT
                     GLSQRVVVEHGREDLQPRVSIKDEHGATVFRCMLPVSAHLMVSEGQMVSAGDSVSKIP
                     RETMKTKDITGGLPRVAELFEARRPKDAAVISEIDGRVELGGIVKGMRKLSVRDEHGE
                     LREYLIPRRRHINVLEGDEVKAGEPFMDGPINPHDILDVLGEQELQKYLVNEIQEVYR
                     LQGVQINDKHIEVIVRQMLKRVLVEASGDANFLIGEHVDKAVFLEENQRAMASGGAAA
                     TAKPLLLGITKASLSTDSFISAASFQETTKVLTEAAINGARDELRGLKENVIMGRLIP
                     AGTGMRWYRETTISTPEVALSKAALTGAIVAVDRGPDELDASSDN"
     gene            complement(7518..>10520)
                     /gene="rpoB"
                     /locus_tag="VN444_01750"
     CDS             complement(7518..>10520)
                     /gene="rpoB"
                     /locus_tag="VN444_01750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWR20565.1"
                     /translation="ELSSFAVLASRVGQDMFEIRENIIRDSTRSEKEGLTEIYRRMRP
                     GDPPNEDATKAFLETIFFNPKRYDLSKVGRLKINRKLGLDVPLEVHTLMCRRYRPAGM
                     EGRTGQVDDIVEAIRYLLRLKHGEAGTSVDDIDHLGNRRIRSAGELLEEQFRVGLARM
                     ERAVRERMSTQELETMMPHDLVNAKPVTAALKEFFGSSQLSQFMDQTNPLAELTHKRR
                     LSALGPGGLSRERAGFEVRDVHPTHYGRMCPIETPEGPNVGLIASLSTYARVNDFGFI
                     ETPYRKVRGMVVTDEIEYLTADEEEKHTIAQANAEVDGRGRFTSDRVSARFDGNFVTV
                     PPTNVDYMDVAPRQLVGVSTSLVPFLEHDDANRALMGANMQRQAVPLLRPEAPVVGTG
                     MEYPAAKDSGAVVVAQRGGIVESVTADRIIIRSSDGKSEDGGADSGVDIYSLVKFRRS
                     NQNTCITQKPIVSKGERIAKGQVIADGPATQNGELALGQNVLTAFMVWGGYNFEDAIL
                     ISERLIKEDRYTSIHIEEFEIEARETKLGKEEITRDTPNVGEDALKDLDESGIVRIGA
                     EVKPGDILVGKVTPKGETVLTPEERLLRAIFGEKAEDVRDASLYVPPGIFGTIVDVKV
                     FSRKGIEKDERAKSIEDEEVSRLRKDFEDEINIIAADRDRKLRSLLDGLSVNREVRSR
                     LTRKVIVPKGKKLTGETLETLPHEDLVDLAGRLDEELGRKASRIGDAADNQVHVLQTL
                     LEERISRATRGDELAPGVFKMVKVYVAMKRKLSVGDKMAGRHGNKGVIAKILPEEDMP
                     YLPDGTPVEVVLNPLGVPSRMNVGQVLETHLGWAARALGLRVASPVFDGATEKEIKAW
                     LKRANLPVTGKTVLYDGRTGKPFHQEVTVGYIYLMKLAHLVDDKIHARSIGPYSLISQ
                     QPLGGKAQFGGQRFGEMEVWALEGYGAAHILQEILTVKSDDVVGRTKMYESIVRGDCT
                     LEPGLPESFNVLVKELQSLALDVELKKE"
BASE COUNT         2158 a         3304 c         2651 g         2407 t
ORIGIN      
        1 cgaaggcgac cttcgcgttc gctgcgtgca ggaccttcgt gatcgccgcg gtcagggtgg
       61 tcttgccgtg gtcgatgtgc ccgatggtcc cgatgttcat gtgctctttc gtccgctcga
      121 acttcgcctt gcccatagtc aactccttta gatatgaaac cgaatccgat tatcgtccgc
      181 cgtgccgtcc acccatacgg gccacaatct ccccagcaat actggcaggg accggttcgt
      241 agcgtgaaaa ctgcatcgaa tgggcggccc gcccctgtgt cgccgaacga aggtcagtgg
      301 cgtacccaaa catctcggag agcggcacat ccgcttgaat cacctgggcg acagatctga
      361 cctcgatccc aatcactcga ccacgacgcg agttcagatt accgataacc tcacccagga
      421 aggcttccgg tgttgagaca tcgactcgca taatcggttc cagcagtacc ggcttcgccc
      481 gcttggtcgc ctctttgaaa gccattgagc cggcgatctt aaacgacatc tccgaggagt
      541 ctacctcgtg atatgatccg tcaaagaggg taaccttcaa atcgatgacg gggtagccgg
      601 ccaccacacc ggtatggagt gcctccttaa tgcctttctc aaccgcggaa atatattctt
      661 tgggcacgac tccacccacg atcttattga caaattcaaa tcccgattga gcggtggagg
      721 gttccacttt gagccagacg tgtccgtact gtccacgccc accggtttgc cgtacatact
      781 taccctcagc ctcagccgtg attgtcacgg tttcacgata ggccacctct ggcttaccga
      841 cattggcctc aacccggaac tcgcgcgtga gcctatcgac gataatctca agatgcagct
      901 ctcccattcc ggagatgatc gtctggccgg tctcttcatc cgtactggcg cgaaatgtcg
      961 gatcctcatt agccaatgct tgaagacctg tcgcgagccg atcttgccct tctttcgtct
     1021 ttggctcaat agccacagag atcaccggct caggaaaaac gatcgattcc agaatgatct
     1081 ggttgccctc atcgcacagg gtgtcgcccg tttttgcgcc cttgagcccc actgcagccg
     1141 caatgtcacc agcaaagacc tctttgatct cctcgcgctt gttagcgtgc atctgcagca
     1201 gccgcccaat gcgctcccgg gtgccccgcg tggagttgta cacgtgactc cctgaggcga
     1261 gggtgccaga gtagacccta aagaaggcca gttgtcccac atagggatcc gtcataatct
     1321 taaacactaa agctgcgaaa gggtcattga ccttcgggac gcgcactaca gatttcgtgc
     1381 gtgtcgaatc gagcccctca atgggcggca ggtcaaccgg ggacgggaga tagtccacca
     1441 cggcatcgag taatagctgg acgcccttgt tcttgaacga ggcgcccgcg aggaccggga
     1501 ccagcaggag cttgagagct gcgctacgaa tcgcggattt gatctcctcc ggtccaaccg
     1561 cgaggccatc cacatatctg acgaggaagc tatcgtccac ttcagcgatg gtctccagca
     1621 tccgctcgcg agcagtctga gccagtgagc tcatctcctc agagatttca tcctcccggt
     1681 agctcgcccc cagggtctca tcgtcccaga tcaccgcctt catccggatc aggtctacga
     1741 caccctcgaa atgatcctca cggccaatgg gaagctgcac aaccaatgga acagctccca
     1801 gacgctcggc gatcattcgc acgcacatat caaaattggc ccccatacga tccatcttat
     1861 taatgaaggc gatacgtgga acaccgtact tatccgcttg gtgccacacc gtctcagact
     1921 ggggttctac gccggccacc gcgtcgaaga gggccacggc accatccagc acgcgaagcg
     1981 atcgctcaac ctcaaccgta aaatcgacgt gcccaggtgt atcgataata ttgatacgat
     2041 ggtctcgcca gaagcaggtg gtggtggctg aagtgatcgt aattcccctc tctcgctcct
     2101 gctccatcca gtccatctca gtggaaccat cgtgcacctc tccgatctta taggtcctgc
     2161 cggtatagta caggatccgc tcggtagtgg tggtcttccc agcatcgatg tgggccataa
     2221 tcccgatgtt tcggaccgtc tctatcggat agatatcagg catctcaaat tccagtttcc
     2281 agtttcagga tcccaaggag tgcacgccaa cttggaatcc ggcacagttt ctcaccagcg
     2341 ataatgggca aatgccttat tcgcttccgc catcttgtgc gtatcttcct tcttcttcac
     2401 cgagcttccg cggttagccg ccgcttcgat cagctcagca gccagccgct ctgccatcgt
     2461 cttctctgta cgctttctgg agaagccgat gatccaacga aacgccaagg agttccgacg
     2521 ctccgctcgg acctctaccg gcacctgata ggtcgcaccc cctactcgac gcgacttcac
     2581 ttcaagtacc ggtttgacgt tatcaacggc ctgcttgaac attcgcaacg gatcggtttt
     2641 cgcccgatcc tcaatgatct tcattgaccc gtacacaatc gactcggcaa cgcttttttt
     2701 accttggacc atcatactat taataaactt cgtaaccatt cggttgctat aaacaggatc
     2761 agcgagcgtc tctcgtttct caattacttt tcgtctgggc attgcgcact ctccgaaata
     2821 gtaagtctca cacgtaacca gaaaattaaa atcggcgctt caggcaaggt ccatgaagaa
     2881 ccgatctctc ttcagtaccg aagggtgtta actaatcttt ggtctcttag cgccgtagag
     2941 agatcggccc tgtttgcgat cctggacacc tgccgtatcg agagcgccgc gaatcacatg
     3001 atagcggacg cctggcagat ccttgacgcg acctcccctg atcagaataa tggagtgctc
     3061 ctgcagattg tgcccgactc ctggaatata cgttgtcacc tcaattccat tactgaggcg
     3121 aaccctggtc acctttcgaa gggccgaatt cggcttcttt ggggtagtcg tatagacacg
     3181 aatacagacc ccacgccgct gcggacaccc ctgtaaggcc ggcgtcttgg ccttcttgac
     3241 ggctgcggat cgacccttac gaaccaactg gtgaatggtc ggcactgctt cctccctctc
     3301 taaggcaatc tagccattat acagactata tgtctattgt caagcccttt aattacttat
     3361 ttaattatca gaagacgcat ccagctcatc cgggccccgg tcaactgcca cgatcgcacc
     3421 cgttaaggcc gccttcgaca gcgccacctc gggtgtggaa atcgtcgtct ctctatacca
     3481 cctcatccct gtgccagccg gaatcagtcg ccccatgata acgttctctt tcaggccacg
     3541 tagttcatcc ctcgccccat tgatggccgc ctctgtcaag accttcgtgg tttcctgaaa
     3601 cgaggcggca gagatgaagc tgtcagtgga aagtgacgcc ttagttatcc ccaggagaag
     3661 aggcttagcg gtggccgcgg ctccgcctga ggccattgcc cgctggttct cttcgaggaa
     3721 gacagcttta tcaacatgct cgccgatcaa gaagttcgca tcgccagacg cctcgaccag
     3781 cacccgcttg agcatctgcc tcacgatcac ttcaatatgc ttgtcgttga tctgcacccc
     3841 ctggagtcgg tagacttctt gtatttcatt tacgagatac ttctgcagtt cctgttcgcc
     3901 caatacgtcc aggatatcat gggggtttat ggggccgtcc ataaagggtt cgccggcctt
     3961 aacctcatcc ccctccaaca cattgatatg cctgcgtcga ggaatcaagt attctctgag
     4021 ctcgccgtgc tcgtccctga cggaaagctt acgcatgccc ttaacgatcc ctcccaattc
     4081 tacacgacca tcgatctcgg agatgacagc agcatctttt ggcctacgtg cctcgaatag
     4141 ctcagccacc cgggggagac cacccgtgat gtccttggtt ttcatcgtct cccgcggaat
     4201 cttcgaaacg ctgtctccag ccgacaccat ctgcccctcg cttaccatga ggtgagcact
     4261 gacaggcagc atacagcgga agacagtcgc cccatgctcg tccttaattg agacccgagg
     4321 ctgcaagtcc tcccgtccgt gctccactac gactcgctga gataggccgg tgacctcatc
     4381 cacttcctcc cggatggtca ctccctcgag gacatctcga aaatgtacct ttccgccgtg
     4441 ctctgtcaag atcgcattcg taaagggatc ccactccatc aacacctgcc ccgctttcac
     4501 cgtcgcacca tccgccacct tcatgtgagc gccgtagacc gctgggtagt tttctttctt
     4561 acggcccttc tcatcgatga tgctgatcgc gccattccga ttcatcacca cgtagtcccc
     4621 ttggggggcc ttaaccgtac gcaggcccgc gaacctgacg attcccgctc ccctacattc
     4681 aagagtactc tgctcgacag aacggctcgc agtgccccct atgtggaaag ttcgcatggt
     4741 tagctgagtc ccgggctcac cgattgactg cgctgccagg acaccgcacg cctctcccaa
     4801 ttccaccagc ctgccggtag caaggttgcg gccgtagcac ctgatgcaga tgccccgtcg
     4861 cgcctcacag gtaaggacag agcggatttt aaccctatcg aggccggcat tttcgatcat
     4921 gctggccagc acctccacga tctcctgatt ggcccccacc aacacttctc cagtaaaggg
     4981 atcaaggatt tcatccaggg ctacccgacc gaggattcgg tcacgcaggg gctggataac
     5041 ctcgcctcct tcgatgaggg gggtaaccca tatcccgttg ggcgtaccgc aatccacctc
     5101 ggccacaatg acatcctgcg ccacgtccac cagacggcgc gtgagatagc cggaatcggc
     5161 ggtctttaat gccgtgtccg ccaggccctt tctcgccccg tgagttgaga taaagtactg
     5221 aaggacagtg agcccctccc ggaagttcgc ggtaatgggg gtctcgatga tctccccgga
     5281 gggtttggcc atcaagccgc gcatgcctgc caactgcctg atctgctgcc ggctgcctct
     5341 cgcaccagag tcagccatca tgaagatcgg gttaaattcc cccttctcct cagcctcgat
     5401 ctctcggaac atctcgtccg acacacgttc agttacgtgg gtccagatgt cgataatctt
     5461 gttgtaacgc tcgcccttgg tgatcaggcc atcctgatat tcacgctcga ccttgacgac
     5521 ctccttgttg gcatcgtcga tcaccttacc tttggtggac gggatgtgca tgtcgtcgat
     5581 gccaatcgag ataccggcga gcgtggcgta cctgaacccg aggtccttca ggttgtccag
     5641 gagcttgacc gttaccgtat tccccaagag atagcagcac tgcgcgacga gacgggtcag
     5701 ctcccgtcta ttcatctctt ggttgacgaa ccgaagcgtc tctggcaact gttcattaaa
     5761 gatgcagcgc ccgacggtgg tctcgatcat ttcgccttcg acgcggacct tgatccgggc
     5821 cagcagagct acttcttcgg cgtcataggc cgcccgaacc tcatccatat cggagaatac
     5881 atagccctcg cccttctcgc cagctctact ccgggtcaga tagtagcaac ccagcacgat
     5941 atcctggctg ggggtaacga cgggtaaacc atttgccgga gaaaggatat tatgggaagc
     6001 cagcatcaga accgaggcct caatctgggc ctccggagac agaggcacat ggacggccat
     6061 ctggtcaccg tcaaagtcag cgttgaacgc cgcgcagacc aggggatgga tcttgatcgc
     6121 ttcgccttcc accagtacgg gttcaaaggc ctgcactccc agccgatgca gcgtcggggc
     6181 acggttcagg aggaccgggt gctcgtgaat cacctcctcc agggcatccc acacctcagg
     6241 cttacccttt tctaccagtt tcttggcact cttgatggtg gtgacgacgc cgtctttgag
     6301 gagcttcctg aagatgaagg gtttgaatag ctcgagagcc atcttctttg gcagaccaca
     6361 ctggtgcagc ttcaattcgg gccccaccac gatcaccgat cgcccggaat agtccaccct
     6421 ttttccgagc aggttctggc ggaagcgccc ctgctttccc ttcagcattt cagaaaggga
     6481 cttgagggga cggttattct ggcccttcag cgcgcggccg cgacggccat tgtcgaacag
     6541 agcgtcaacc gcttcttgca gcatccgctt ctcgttacga acgatgatat caggtgcccg
     6601 gagttcaatg agacgcctga gtcggttgtt gcgattaatt acccgtcgat agagatcgtt
     6661 caggtccgat gtggcaaaac gccccccgtc aagagggacc agcggccgaa gctcgggtgg
     6721 caggactgga atcgcgtcca gaatcatcca ttccggccga ttgctggagt tgatgaaggc
     6781 ctccacgatt cgcagccgct tggtgatctt cttacgaccc tgttgagagg tctcttgggg
     6841 catctgcgcg tgaagctctt ccgccagctc gcgaagattc agccgcttca acagatcccg
     6901 aattgcccct gcgcccatcc ccgccttaaa gccgtccccg tactcttcgt aggccaggcg
     6961 atgctgctcc tcggtcaaga gctgtttcaa tgagagagga gtcttacccg ggttcagcac
     7021 cacatacgct tcgaaataga ggaccctctc cagatctcgg agggatatat caaggagata
     7081 gccgatccga ctgggtacgc ctttgtagaa ccagacgtgg accaccgggg agaccagctc
     7141 gatgtggcct agccgctgcc gtcgcgcctt gctcctgatc acctcgacgc cgcacttgtc
     7201 gcagacaact cccttatgct tgatgccctt atacttaccg cagttgcact cccagtcttt
     7261 tgtcggaccg aagatcttag agcagaagag cccatctcgc tccggcttaa acgtccggta
     7321 gttaatggtc tccggtttct tcacctcccc gaaggaccac gaccgaatct tctctggtga
     7381 agcaagtccg attcggatgg ctttgaaact ggtgggatcg atggtctttt cgtcgtagaa
     7441 cccgaaaaac ttatccaata ctaccacctc cacataccct accgattgcg gaattctgca
     7501 ctccgcactc cgcagggcta ctcctttttc agctcgacat ccagcgcaag gctttgtagc
     7561 tccttgacca ggacattgaa ggattccggg agtccaggct cgagggtaca gtcgcccctg
     7621 acaatcgact cgtacatctt cgttcgcccc accacatcgt ctgacttcac ggtcaggatc
     7681 tcttgtagga tgtgggcggc cccgtagccc tccagggccc acacctccat ctctccgaat
     7741 cgctgtccgc cgaattgagc cttaccccct aggggctgct gagagatcaa tgagtaggga
     7801 ccaatggagc gagcgtgaat tttgtcatcc acgagatggg ccaacttcat gaggtagatg
     7861 tagccaaccg tcacctcctg gtggaacggc ttcccggttc gtccgtcgta gaggacagtc
     7921 ttccccgtga cggggagatt ggctcgcttg agccaggcct tgatctcttt ctccgtagct
     7981 ccatcaaaga ccggtgaggc gacccgcagc cccaaggccc tggccgccca gcccagatgg
     8041 gtctctagga cctgacccac gttcatcctg gaaggtacgc ccagaggatt caggaccacc
     8101 tcgacaggcg tcccgtcggg tagatagggc atatcctcct cggggaggat cttcgcaata
     8161 acgcccttgt tcccatgtcg accggccatc ttatcgccga cagagagttt tcgcttcatg
     8221 gctacgtaga ccttgaccat cttgaagacc ccaggagcga gttcatcacc tcgggtcgct
     8281 ctgctgattc gttcttccag gagcgtttgc aagacgtgga cctggttatc ggccgcatca
     8341 ccgatcctgc tcgccttccg acccaactct tcgtccaatc gccctgcgag atccaccaga
     8401 tcctcatggg gcagcgtttc cagggtctcg ccagtcagct tcttgccctt gggaactata
     8461 accttacgcg tgagcctact ccggacctcc ctgttgacac ttagaccatc gagaagggag
     8521 cgcagcttcc tatcccgatc tgcagcaatg atgttgatct cgtcttcgaa gtccttgcgg
     8581 agtcgactga cctcttcgtc ctcgatcgac ttggcccgct cgtctttctc gatccctttt
     8641 cgggagaaga ccttcacatc gacgatggtg ccgaaaatcc caggtgggac atagagcgaa
     8701 gcgtcccgga catcctcggc cttctcgcca aagatagctc gcaacaatcg ctcttctgga
     8761 gtaagcacgg tttcaccttt gggggtaacc ttccccacca gaatatcccc gggcttcacc
     8821 tcagctccga tccgaacgat cccactctcg tcgagatctt tgagcgcatc ctcacccacg
     8881 ttcggggtgt ctcgcgtgat ctcctctttc cccagcttgg tctcgcgagc ttcgatctcg
     8941 aactcctcga tgtggataga ggtatagcga tcttccttga tgagccgctc actgatcagg
     9001 attgcatcct caaagttata cccaccccat accataaatg ccgtcaggac attctgcccg
     9061 agcgccagtt cgccgttctg agtcgccgga ccatctgcga tcacctggcc cttggcgatc
     9121 cgctctccct tgctcacaat cggcttctgc gtgatgcagg tattctggtt gctacggcga
     9181 aacttcacca gagagtagat atctacccct gaatcggccc cgccgtcttc actttttccg
     9241 tccgaggagc ggatgatgat ccgatcagcg gtcaccgatt cgacaatccc tccacgctgg
     9301 gccaccacga ccgccccaga atccttcgcc gcgggatatt ccattccggt tccgaccact
     9361 ggagcctccg gtcgcaggag ggggacagcc tggcgttgca tgttagcgcc catcagggca
     9421 cgattggcgt catcgtgttc aaggaatggg acaagcgaag tagacactcc aacaagttgc
     9481 ctcggggcga catccatgta atcgacattg gttgggggaa ccgtgacaaa gttaccgtcg
     9541 aatcgtgccg agacccgatc tgacgtgaaa cgtccccgtc cgtctacctc cgcgttggcc
     9601 tgtgcaatag tgtgcttctc ctcttcatcg gccgtcaggt actcgatctc gtcggttacg
     9661 accatacccc ggaccttacg ataaggcgtc tcgatgaagc caaaatcgtt gacgcgggca
     9721 taggtagaga gactggcgat aagaccaacg ttggggcctt ccggcgtttc gatgggacac
     9781 atccgaccgt agtgtgtcgg gtgtacgtcc cggacctcga accctgcccg ctcccgcgac
     9841 agccctccag gacccaaggc tgacagccgc cgcttatggg tcagctcagc gagcgggttg
     9901 gtctggtcca tgaactgaga gagctgggag ctgccgaaga actccttcag cgccgccgta
     9961 accggcttgg cattcaccaa gtcgtgtggc atcatggtct ccagctcctg tgtactcatt
    10021 cgctctcgga ctgcccgttc cattcgagcc aagccgaccc ggaactgttc ttccaggagt
    10081 tccccagccg accgaatccg gcggttgccc agatggtcga tgtcatccac ggaggtcccg
    10141 gcctctccgt gcttcagtct gaggaggtat cggatggcct ctacgatatc gtctacctgg
    10201 cctgtccgtc cctccatacc ggcaggccga tatcggcggc acatgagggt atgaacctca
    10261 agcggcacgt cgaggccaag tttgcggttg atcttcaggc gcccgacctt ggaaagatcg
    10321 tagcgcttcg gattaaagaa gatggtttcg aggaacgcct tggtcgcgtc ctcgttaggc
    10381 ggatcccccg gtcgcattct tcgataaatc tcggtcaggc cctccttttc agagcgagtg
    10441 gagtcgcgga taatgttctc ccgaatctcg aacatatcct gaccaactcg gctggcaagc
    10501 acggcgaagc tgctcagttc
//
LOCUS       DATMXI010000094         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_164972, whole genome shotgun sequence.
ACCESSION   DATMXI010000094 DATMXI010000000
VERSION     DATMXI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164972"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VN444_01755"
     CDS             complement(<1..814)
                     /locus_tag="VN444_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWR20566.1"
                     /translation="MGRSLRIIGGEVYDPANGIDGAIKEICVEDGKVVQSCTGPAEVI
                     DATNLVVMPGGVDIHTHIGSPAVNAARGFRPEDHRHVHFSKKPGLRSGVGSTVPSTFA
                     TGYLYAQMGYTTVMEGANAPIGVRHTHEELIDTPIVDKGLYIELGSNEFILKFIKAGE
                     MEKVKRYVAWLIRSLKAYGVKLVNPGGVEQWKYGKDVKNLDDKVHYFDVTPRQILKAL
                     AWANESLGLPHSIHLHANSLGTPNNYLTTLDTMQTLEGSRLHVAHAQFHSYGG"
     gene            complement(826..>2080)
                     /locus_tag="VN444_01760"
     CDS             complement(826..>2080)
                     /locus_tag="VN444_01760"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HWR20567.1"
                     /translation="VKVENGKITEVKNACVLGKATFLHHLEGLSTPRLYGKPATIDEC
                     IDAAAGILAKAKYPLIYGLDSTELGAQRASIELADLIGANIDHTSSVCHAPSYQAVMT
                     VGIPTCTSGETKNRADLVIFWGCNPAEAHPRHATRYSVTPKGMFTPKGKKDRMVIQVD
                     VRPTPTTRIADAFIQMKPNSDYEVISALRALVKGHELEQAEVGGIAVDELKALVEKMK
                     SCKFGIVYWGMGLTQTRGKYLNLVALLKLAQDLNEFTKFSCAAMRGHGNVVGMAQVLT
                     WQTGYPFHVNMSRGYPRFNPGEFSLVDLLARKEVDAALIVAGDAIGNFPGSLAEHLRS
                     IPLIAIDPKESDTTKVADIVIPSAQGAIGAGGMAYRMDHIPLMFKKVVESPYPSDREI
                     LDRIIAKVKAMKSRGNGAPAPAGRG"
BASE COUNT          415 a          644 c          554 g          467 t
ORIGIN      
        1 ccccgccata actgtgaaac tgggcgtgcg ccacatggag ccttgagccc tcgagggtct
       61 gcatcgtgtc cagggtcgtc aggtagttgt tgggtgtgcc caagctattg gcgtgcaggt
      121 ggatcgagtg gggcaatccg aggctttcgt tggcccaggc gagcgccttc agaatctggc
      181 gcggcgtgac gtcgaagtag tggaccttgt cgtccaggtt cttcacatcc ttgccgtatt
      241 tccattgctc taccccgccg gggttgacca gcttgacgcc atacgccttt agcgaccgga
      301 tgagccaggc gacatatcgc ttgaccttct ccatctcgcc cgctttgatg aacttcagga
      361 taaactcgtt actccccaat tcgatataca gccccttatc cacgatcggg gtgtcgatca
      421 actcctcgtg ggtatgccga acgccgatgg gagcgtttgc accttccatc accgtggtat
      481 agcccatctg ggcgtagagg tagccggtcg caaaggtgga gggaaccgta gaaccaacgc
      541 cggacctgag gccgggcttc ttgctgaaat gcacgtgccg gtgatcttcc ggcctgaaac
      601 cgcgagccgc gttgaccgcc ggactgccga tatgcgtatg aatatcgaca ccgcccggca
      661 ttacgaccag attcgtagca tcaataacct cagcgggccc ggtacaggac tgcacaacct
      721 ttccatcctc aacacagatc tctttgatgg ctccgtcgat cccgttagcc gggtcataga
      781 cctcaccgcc gatgattctt aacgagcgtc ccatgtcgat tatcctcagc ctctaccggc
      841 cggggcggga gcgccattgc cgcgactctt catcgccttc accttcgcga tgatacggtc
      901 gaggatctcc cgatccgatg ggtagggtga ttcaaccacc tttttgaaca tcagggggat
      961 atggtccatc ctgtacgcca tccccccggc accaattgca ccctgcgctg acggaatcac
     1021 gatgtcggct actttggtcg tatcgctctc tttggggtcg atggcaatca acggaatcga
     1081 tcgcagatgc tctgccaggc tcccgggaaa gttcccgatg gcgtctccgg cgacaatcag
     1141 cgcggcatcg acctctttac gggccaacag atcgaccaac gagaattccc cagggttgaa
     1201 tcgaggatag ccgcgactca tattgacatg aaagggatag cccgtttgcc acgtcagcac
     1261 ctgagccata cccaccacat tgccatgccc cctcatcgcc gcacacgaaa atttcgtaaa
     1321 ctcgttgaga tcctgagcaa gcttgagcag cgcgacgaga ttcagatatt ttcccctggt
     1381 ctgggtcagg cccatccccc aatagacgat cccgaactta cagctcttca tcttttctac
     1441 aagagctttc aattcatcga ccgctatgcc tccaacctcg gcctgttcca attcgtggcc
     1501 cttcacaaga gccctgagag cagagatgac ctcatagtcg gagttcggtt tcatctggat
     1561 gaaggcgtcg gcgattcgcg tggtcggcgt cggcctcaca tcgacctgaa tgaccatccg
     1621 gtccttcttc cccttcgggg tgaacatccc cttcggagtc accgagtacc gagtggcatg
     1681 ccgtgggtgc gcctcggcgg gattacatcc ccagaagatt accagatcgg cccggttctt
     1741 cgtctctccc gacgtgcagg tcgggatgcc gacggtcatt accgcctgat agctcggcgc
     1801 gtggcagaca gaagaggtat gatcgatatt cgcgccgatc agatcggcca gttcgatcga
     1861 tgcccgctgc gcacccagct cggtcgagtc gagtccatag atgaggggat acttggcctt
     1921 cgcgagaatc cctgccgccg cgtcgataca ctcatcaatc gtcgccggtt tcccgtaaag
     1981 cctcggcgtc gacaatccct ccaagtgatg gagaaaggtg gctttaccca ggacacaggc
     2041 gttcttgacc tccgtgatct tcccgttctc gaccttgact
//
LOCUS       DATMXI010000095         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_129275, whole genome shotgun sequence.
ACCESSION   DATMXI010000095 DATMXI010000000
VERSION     DATMXI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129275"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VN444_01765"
     CDS             complement(<1..241)
                     /locus_tag="VN444_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HWR20568.1"
                     /translation="MMQQSDVLQEKVKGLPDAPGVYLFRDPRGHVLYIGKALSLKKRV
                     SSYFTEAPDVPERSQIRLMMEQVTDLEFIITANELE"
     gene            399..587
                     /locus_tag="VN444_01770"
     CDS             399..587
                     /locus_tag="VN444_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20569.1"
                     /translation="MAGKFQPYRGERRRKETARKTKKEEKLRKKLGKKEGALPTEESE
                     TTEGSPPADEPPPHPISS"
     gene            complement(608..2437)
                     /gene="typA"
                     /locus_tag="VN444_01775"
     CDS             complement(608..2437)
                     /gene="typA"
                     /locus_tag="VN444_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWR20570.1"
                     /translation="MSQQRTDLRNLAIIAHVDHGKTTLVDAMLKQSHIFRDNQVMIER
                     VMDSNPLERERGITILAKNTAVTYGKVKINIVDTPGHADFGGEVERVLNMVDGVLLLV
                     DAVEGPMPQTRFVLRKALELHHKVVVVVNKIDRANARIDYATNATFDLFIDLGATEEQ
                     TDFPIVYTDGLRGRAGLSPTSLAPDLQPLFETILSSLPPPMVERDGPTQMLVNLLAYD
                     DYKGRIAIGRLYAGRLRRAQEVIRIAKDGTCYPGKIAQVFVHQGLDRVEVEEAEAGEI
                     VAVTGLAEIGIGETIADKDTPIALPPLLVDEPTVQMTFSVNTSPFAGREGKWSSSRSL
                     RERLFKELEHNVSLRVYDTESPDSFLVAGRGELHLVVVIETMRREGYELQVSRPEVIF
                     QTDLQSGERLEPFERVSIEVPEEQAGLVMEMLGVRRGRLQDMRTGDQGFVHYEFTVPT
                     RGLLGFRQAFLTATRGTGLLNSLFHAYEPMVGEIRGRDRGSLVAWEAGVATSYGLASA
                     EASGILFIGPGTEAYEGMIVGEAAKERDLEVNVAKRKHLTNMRASTSDIAVRLTPPRQ
                     MSLDNCVEYLAEDELLEVTPKSLRLRKKVLDRHGRKRVRVAGR"
     gene            2804..>3268
                     /locus_tag="VN444_01780"
     CDS             2804..>3268
                     /locus_tag="VN444_01780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWR20571.1"
                     /translation="MKRTRYVSMLTTTLVMILALTASLSGCSPGSGSQTVPPETAPVK
                     AKTGDPAIGNGSTVKLEYTLTDDKGALLDTNKEKEPLVYTHGQGQIIPGLEKALIGLR
                     TGDAKHVVIRPEEAYGPIRPEAVVEVPKERIPEKLQTVGAHLVAQRKDGQPMH"
BASE COUNT          674 a         1022 c          948 g          624 t
ORIGIN      
        1 cctccagctc gtttgccgtg atgatgaact ccaggtcggt cacctgctcc atcatgagcc
       61 ggatctggga ccgctctggt acgtccggcg cttccgtaaa gtaggaggag acgcgtttct
      121 tcagcgacag ggccttgccg atgtacagca cgtgccctcg tgggtcacgg aatagataga
      181 cgcctggcgc gtccggcagg cctttgacct tttcttgaag cacatcgctc tgctgcatca
      241 tagctgaaag atgtcatagg cacccgacgc tgtcaaggca cgctgccgct caagaccctt
      301 gacatcgtta tcccgaaggc gtaggctatt ccatagttct tgcggcttcg tccgaggctg
      361 cgaaagagaa ggaaacgatc gcggaaagga gaaagtgaat ggcgggaaag tttcaaccgt
      421 accgagggga gaggcggcga aaggagaccg cacgcaagac caagaaggag gagaagctcc
      481 gaaagaagct ggggaagaag gaaggggctc taccgaccga ggagagtgaa actacagagg
      541 gctcgcctcc cgccgacgaa ccgccacccc acccgatctc atcatagtgt cgggtggggt
      601 cagcgcgtca tcgccccgcg accctcaccc gctttctacc atgcctatcc agcaccttct
      661 tgcgtagccg tagactcttc ggtgtgacct ccagcaattc gtcttctgcc aaatactcaa
      721 cgcaattgtc gaggctcatc tggcgtggcg gcgtcagacg cacggcaata tcagaggtcg
      781 aggctcgcat attcgtcagg tgcttccgct tcgcgacatt gacctccagg tctcgctcct
      841 ttgccgcctc gccgacgatc atcccttcat acgcctcggt tcctggtccg atgaaaagga
      901 tcccgctcgc ctccgcactc gccaggccgt aagacgtagc cacccccgcc tcccacgcga
      961 ccagcgaccc gcggtcacgc ccgcggatct cgccgaccat gggttcatag gcgtgaaaca
     1021 ggctgttgag cagcccggtc ccgcgcgtcg cggtcaggaa cgcctgccga aatcccagca
     1081 accctcgcgt ggggaccgtg aactcatagt ggacgaaacc ctgatcaccc gtccgcatat
     1141 cctgcagacg ccctcgtcgc actcccaaca tctccatcac cagccctgcc tgctcctccg
     1201 gcacctcgat gctgacccgt tcgaacggct ccaaccgctc cccgctctgc aaatcggttt
     1261 gaaagatgac ctcgggcctc gagacttgca gctcataccc ctcgcgtcgc atcgtctcga
     1321 tcacgacgac caggtgtaac tcgcctcgtc cggccaccag gaagctgtcg gggctctctg
     1381 tgtcatagac tctcaggctg acattgtgct cgagctcctt aaacagacgc tcgcggaggc
     1441 tgcggctcga gctccacttc ccctcccgtc ccgcaaaggg gctggtattc acgctgaagg
     1501 tcatctgtac ggtcggctcg tctaccagga gcgggggaag ggcaataggg gtgtccttgt
     1561 cggcaatcgt ctctccgatc ccaatctccg caaggccagt caccgccaca atttcgccgg
     1621 cctccgcctc ctcgacctcg actcggtcca acccctgatg cacaaagacc tgagctatct
     1681 taccggggta gcaggttcca tccttcgcga tccggatgac ctcctgagct cggcgcagac
     1741 ggccggcgta caggcggccg atggcgatcc ggcccttata atcatcatag gccaggaggt
     1801 tgaccagcat ctgcgtggga ccatcccgct cgaccatggg gggaggcagg gagctgagga
     1861 tggtctcgaa caggggctgc aggtcagggg ccagtgaggt tggggaaagg cctgcccgtc
     1921 cacggaggcc atccgtatag acgatcggga aatccgtctg ctcttctgtt gcgccaagat
     1981 cgatgaagag atcgaaggtc gcgttggtcg cgtagtcgat tcgggcgttc gcgcggtcga
     2041 tcttgttgac aaccaccaca accttgtgat ggagttccaa ggccttgcgc agcacgaacc
     2101 gggtctgggg catcggcccc tccacagcat ccaccagcag caacaccccg tccaccatgt
     2161 tcaggactcg ctcgacctcg cccccgaagt cggcatgccc tggcgtgtcc acgatgttga
     2221 ttttcacttt cccataggtg acggcggtgt tcttggccag gatggtgatg ccgcgctctc
     2281 gttccagagg gttggagtcc ataacccgtt cgatcatcac ctggttgtcc ctgaagatat
     2341 ggctctgctt cagcatggcg tccacgagcg ttgtcttgcc atggtccacg tgcgcgatga
     2401 tggccagatt cctgagatcg gtgcgctgct gtgacataat gtgccttctt cgatgcggtt
     2461 ataggctgcg ccgtgcccgg cagtgggcgg cacgaatgcg gaaaacgtaa cgtacgctca
     2521 aaactcagta tggggaggcg cgcgaaaggt cagagcccga agggaaaccg gctgcggacc
     2581 tgctgaggcc gttctaacag atgcggcgtg tcaagctgca acggcacgag tcaatcacct
     2641 catgaatcca taacactata tctcaaaggg gcgggttcgt caaacaattc cagcgccgag
     2701 cgtggtgaga cttttcggtt gcgccgcgcg gctcggaggc gtactatgcc ccggtacgcg
     2761 tgagcgattc agttgactgt gtcacaccca tggaggatga agcatgaaga ggacccgata
     2821 cgtatcgatg cttacgacta ccctggtgat gatccttgcc ctgacggcga gtctttcggg
     2881 ctgcagccca ggttcaggca gccagaccgt gccgcccgaa acggcgccgg tcaaggccaa
     2941 gactggcgac cccgcaattg gtaacggctc caccgtgaaa ctggagtaca cactgaccga
     3001 tgacaagggc gcactcctgg acaccaacaa ggagaaggag cccttggtgt acacccacgg
     3061 gcagggacag atcatccctg ggctggagaa ggcgcttatc ggcttgcgca caggcgacgc
     3121 aaagcacgtt gttatccggc ccgaagaggc ctacggaccg atcaggcccg aagccgtcgt
     3181 cgaggttccc aaagaacgaa ttccggagaa attacagaca gttggggccc atcttgtggc
     3241 ccaaaggaag gacgggcaac ccatgcac
//
LOCUS       DATMXI010000096         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_258152, whole genome shotgun sequence.
ACCESSION   DATMXI010000096 DATMXI010000000
VERSION     DATMXI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258152"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VN444_01785"
     CDS             complement(<1..685)
                     /locus_tag="VN444_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR20572.1"
                     /translation="MARSEGEMGSTEGNPTITIGQLLQDARTAKGLTIEAAAAASKVP
                     LSFVRVMEQEQFHLVPDLLYLIRFMTEYCVFLGLDPKQVAAQWKDQANAARMSGLSHP
                     VPSIGSRIDFRRLAMYLVPAVVAIPLIFIGLSLFSGRPPAPPLESQQTTEPTPEAGTA
                     ALPGPQAAAVGAGQPEGVNPARPLTDPAVQRPQGPPPRYALKAEAKQKTWLSVAVDGA
                     PRKGVLLYPG"
     gene            complement(996..1592)
                     /locus_tag="VN444_01790"
     CDS             complement(996..1592)
                     /locus_tag="VN444_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20573.1"
                     /translation="MKIGIDLDDVLADSLPHYLQAFNRRFGLDVELADGAWRIADRFP
                     HIPRQEADDFFTELIDDGFFSSRSLIPHAKEAVETLADDGHDLYIITGRTPRDERITM
                     DWLTRVGVLSHFEAVMHRTREPVGHHKSSAASELQLDLFIEDELAVALAVAETAIPVL
                     LFDHPWNHGPLPGNVHRIRSWGEALAQIGKLNGGQGDR"
     gene            complement(1589..>4465)
                     /locus_tag="VN444_01795"
     CDS             complement(1589..>4465)
                     /locus_tag="VN444_01795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWR20574.1"
                     /translation="PLLIIAGAGTGKTTVIAHRIAHLINSKRVRPEQVLALTFTEKAA
                     AEMERRVDLLVPYGFTDTWISTFHAFGDRVLREHALVLGLSPDFRLLTAAEQIIFFQE
                     HLSEFPLDYFRPLGNPMRHVQALLQLFSRAKDEDIGPEKYASYAEELARRATVSPDDK
                     ALAERAEREVEIARTYQQYQELMAREGKLDFGDLMTRTLALLREHPLVLQRLQERFRY
                     ILVDEFQDTNYAQFQIVKLLTASHRNVTVVGDDDQSIFKFRGASISNILSFRREFPET
                     ETVVLLENYRSTPSVLDAAYRLIQHNNPDRLEYQEGVDKCLRPCSSDGPAVESIVFQT
                     YQEEADWVAGKVEELIEAKACALSDIAILVRRNSDADPLLRALNMKGIPWSFSGARGL
                     YDREEIRLVISFLRLLADPHDSLSLFHLAGSDIYGMPAADLTLCNAYARRKHRSLYEV
                     LLDLTRRPGAVPELSGVSTEGIAAATALLSDLHRYLDKAAREVTGRILYEFLMEQPGY
                     GKRLLHSNSPRDHQRVGNLALFFGLVQRFGEVAPQDRVAGFVPYLTMLIEAGENPPVA
                     EGEAAEEEVAVLTLHKAKGLEFEAVFLVGLVEKRFPSIDRREAIPLPDELIKETLPSG
                     DFHLQEERRLFYVGMTRAKRYLFLTRARDYGTKREWKRSRFLVEAMALPKEEEEQPWR
                     GTAEAVIGRYAPPPQVAIDLAIRTDTPLNLSHYQIDDYLTCPLKYKYVHLLRVPVLRD
                     HTVIYGAALHRAVQAYNTHRLAGQTMSLEDLIDTFEAHWVNEGFISREHEERRLQEGR
                     DALARFHASAGTKQPPTSVEKRFRFQLGDDIVTGIMDRVDEREGETVIIDYKSSSVRD
                     QKAADKGARESQQLGLYALAYRESAGRLPDRVELHFLTPGGVIVGQAVKKEKDLDQAI
                     DRIREAATGIRGGLFQAKPSQWVCDFCVFRTICPATVWIGDRQG"
BASE COUNT          896 a         1416 c         1263 g          890 t
ORIGIN      
        1 gacccggata gagcaggact cccttgcgag gcgccccgtc aactgcgacc gaaagccagg
       61 tcttctgctt cgcctcagcc ttgagcgcat agcgaggcgg tgggccctga ggcctctgca
      121 ccgcgggatc agtgagtggg cgggctggat tcaccccttc tggctgcccc gcgccaacag
      181 cggcggcttg aggaccgggt agggctgcgg tgccggcctc cggggtcggc tcggtcgtct
      241 gctgagactc tagcggcggc gctggcggtc gcccggagaa aagcgagagc ccgatgaaga
      301 tcaaggggat cgcaacgacg gccggcacca ggtacatagc caggcgacgg aaatcgatcc
      361 gcgaacctat tgatggcacc ggatgcgaca gcccgctcat ccttgccgca ttcgcctggt
      421 ccttccactg cgccgcgacc tgctttggat caaggccaag aaacacgcag tactccgtca
      481 taaatcggat gaggtataac agatctggaa ccaggtggaa ttgctcctgc tccatcaccc
      541 gaacgaagga gagaggaacc ttactggcgg cagcagccgc ttcgatcgtc aggccctttg
      601 ccgtcctggc gtcctgcaag agctggccga tcgtgatcgt cggattgcct tcagtgctac
      661 ccatttcacc ctctgatcgt gccaccagtg ttccctttca ttgatgaccg aagcatgctc
      721 ttccgtgcgg ggctgaacgt tcgatccatg ttatcgacaa atggtcacta ctcaggtgtt
      781 taggctgtag actgaaggct gttagtcgtt acgcatggac cgaatcaagg tgcccagaac
      841 cttttcgatc tccatcgttt tcgcgtcata ttcgaaaacg cctggctcat ccgtgcgcgt
      901 tcctacagcc taacagccta tagtctatct acctaagtag ttacgacaat tgtacgctac
      961 cataccggcg catcagaggc aagcggagcg caaggctatc ggtctccctg gcctccgttc
     1021 agtttgccga tctgcgccag cgcctcgccc catgaccgaa tccggtgcac gttgccaggg
     1081 agcgggccat gattccacgg gtgatcgaac agcaggactg gaatagccgt ttccgccacg
     1141 gcaagagcga ccgccagctc atcctcgatg aagagatcga gttgtaattc tgatgccgca
     1201 ctcgacttat ggtgtccaac cggctcgcgc gtcctgtgca tcacagcctc aaagtgagac
     1261 agtacgccga cgcgcgtcag ccagtccatc gtgatcctct cgtctcgcgg ggtccggccg
     1321 gtgatgatat acagatcatg gccgtcgtcg gccaatgttt ccaccgcttc tttcgcgtga
     1381 gggatcagcg atctggatga gaagaagccg tcgtcgatca gttccgtgaa aaaatcgtcg
     1441 gcttcctgcc gcggaatatg aggaaacctg tcggcgatcc gccaagcgcc atcagcaagc
     1501 tcaacatcaa ggccaaagcg tctattaaac gcctgcaggt aatgcgggag tgaatcggcc
     1561 aggacatcat cgaggtcgat accgatcttc atccctggcg atccccaatc cacacggtag
     1621 cggggcagat ggtcctgaag acgcagaaat cgcataccca ttgactgggc ttcgcctgaa
     1681 agaggcctcc acgaatcccg gttgcggcct cccgaatccg atcgatcgcc tgatcgagat
     1741 ccttttcctt cttcaccgcc tgcccgacga tgacgccgcc aggggtcagg aagtgcaact
     1801 ccacccgatc cggtaaccgg ccggccgact cccggtaggc cagcgcatac agcccaagct
     1861 gttggctttc ccgagcccct ttgtctgctg ctttttgatc ccggaccgac gatgacttat
     1921 agtcaatgat cacggtctcg ccctcacgct cgtccactcg atccataatg ccggtgacga
     1981 tatcgtcacc gagctggaat cggaaccgtt tctcaacaga cgtcggcggc tgcttcgtcc
     2041 cggcactggc atgaaaccgg gcgagggcat cccgaccttc ctgcaaccgc cgctcttcat
     2101 gctcccggga gatgaaccct tcattgaccc agtgggcttc aaaggtgtca atcagatctt
     2161 caagcgacat cgtctgcccg gcaaggcggt gggtattgta cgcctgaacg gcccgatgca
     2221 gtgcggcgcc atagatgacc gtatgatccc tgagaacagg cacccgcagg agatggacgt
     2281 atttgtattt caacgggcag gtcaggtagt catcgatctg atagtgggag agattgagcg
     2341 gtgtatcggt ccggatcgca agatcgatgg cgacctgggg cggcggcgcg tatcgaccga
     2401 tgaccgcctc tgccgtccct cgccaaggct gctcctcctc ctctttcggc agcgccatcg
     2461 cctccaccag gaaccggctg cgcttccatt cccgctttgt cccgtaatct ctggcccgcg
     2521 tgagaaacag gtatcgcttg gcccgagtca tgccgacgta gaacaggcgc cgctcctctt
     2581 gcaggtgaaa gtcgccggac ggtagcgtct ctttgatcag ctcatcggga agcgggatcg
     2641 cctcccgccg atcgattgag ggaaaccgtt tctcaaccag gcccacaaga aacacggcct
     2701 caaactcgag gcccttggcc ttgtgcagcg tgagcacggc cacctcttcc tccgccgcct
     2761 caccctccgc gacgggcggg ttctctcccg cctcgatcaa catcgtgaga tacgggacaa
     2821 agccggccac gcggtcctga ggcgccacct cgccaaaccg ctgaaccagg ccgaagaata
     2881 gagcaaggtt cccgacccgt tgatgatcgc gtggggagtt cgagtggagc agtctcttcc
     2941 cgtagcccgg ttgttccatc aggaactcgt acaggatacg tcccgtcacc tcccgagccg
     3001 ctttgtccag atagcgatgc aggtcgctca ggagggccgt ggcagcagca atcccttcgg
     3061 tcgagacccc cgaaagctcg ggtacggcgc caggccgcct cgtcagatcg agcagcacct
     3121 cgtagaggct tcgatgcttt cgacgcgcgt aggcgttgca gagggtcaga tccgccgccg
     3181 gcatcccgta gatatcggag ccggccagat gaaagaggct cagggagtcg tgtgggtcag
     3241 ccaggaggcg gagaaacgag atcaccagcc gtatctcctc cctgtcgtac aggcccctgg
     3301 caccgctgaa gctccacgga atccccttca tgttcagcgc ccgcagcagc gggtcagcat
     3361 cgctgtttct ccgaacgaga atggcgatgt cgctaagcgc gcacgccttc gcttcaatca
     3421 gctcctcgac cttcccggcc acccagtcgg cctcctcctg ataggtctgg aagactatac
     3481 tctcaacggc cgggccgtca ctgctgcaag gcctgaggca cttgtcgacg ccttcctggt
     3541 actccaggcg gtccggatta ttgtgttgga tcagacggta cgcggcatca aggacgcttg
     3601 gcgtcgagcg atagttctcc aacaggacga ccgtctccgt ctcggggaac tcccggcgga
     3661 agctgaggat gttactaatt gaggcccctc gaaacttgaa gatcgactgg tcgtcatccc
     3721 ccaccaccgt cacattccga tgggaggccg tgagcagctt cacaatctga aactgggcgt
     3781 agttggtatc ctggaactca tcgaccagga tataccggaa ccgctcctgc aaccgttgga
     3841 gtaccagcgg gtgctcccga agcaaggcaa gtgtccgcgt catcaggtcg ccaaagtcta
     3901 acttcccttc ccgagccatc agctcctgat actgttggta ggtcctggcg atctccacct
     3961 cccgctctgc ccgctccgcc agagccttat cgtcaggaga caccgtcgct cgacgtgcca
     4021 gttcctccgc gtaactcgcg tatttctcag gaccgatatc ctcgtcctta gctcgactga
     4081 acagttggag cagcgcctgc acatgccgca tcggatttcc gagtggccgg aaatagtcta
     4141 gtggaaactc ggacagatgc tcctgaaaga agatgatctg ctccgccgcc gtgagcaggc
     4201 ggaagtccgg actcaacccc aacaccaagg cgtgctctcg aagcacacgg tctccaaagg
     4261 cgtggaaggt ggagatccag gtatcggtga acccgtatgg taccagcaga tccacgcgcc
     4321 gctccatctc agccgccgcc ttctcggtaa aggtcagcgc cagcacctgc tccggacgaa
     4381 cccgcttgct attgatcagg tgggcgatcc ggtgagcgat caccgtggtc ttccccgtcc
     4441 cggcgcctgc aatgatgagc aaggg
//
LOCUS       DATMXI010000097         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_297576, whole genome shotgun sequence.
ACCESSION   DATMXI010000097 DATMXI010000000
VERSION     DATMXI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297576"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..365)
                     /locus_tag="VN444_01800"
     CDS             complement(27..365)
                     /locus_tag="VN444_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR20575.1"
                     /translation="MISKEEFRDPFERINPETAKGLIDKGGMVVVDVREAAEWSHGHI
                     AESVHIPLGTLMNRPRELLQQDGIIFVCAEGIRSAVACEVAAAIGKTQLYNLEGGTIA
                     WLKQGYPLTR"
     gene            complement(497..1567)
                     /locus_tag="VN444_01805"
     CDS             complement(497..1567)
                     /locus_tag="VN444_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWR20576.1"
                     /translation="MDHWLRIGRMCWTAVAIVAISASAVGAAEQENAIVKEEFLVQGR
                     DQGVKLFVREKRLSNLPKIAKENVVLFVHGLPGPGSVIFDLALPGYSWLEYVAERGFD
                     AFTVDIRGFGRSTRPPEMKESPDQNLPLVRADQVMRDIDVAVDYIRSKRKVDRVNIIG
                     HSIGASWSALYATMHPEKVNKLVMYGAIYGKDSTFVSTFGDPTNPDRPYLEMGAYRYL
                     SRKVLLEQWDGWIKPELQDDWRDREVIDAWISTLLSSDPTAKQRSPESIQVPNGPYID
                     WHEIHAGRSLFDPARIKAPTMIVRGSAEELMSNEAAEDLLQKLTSAPSKRRLDIGDST
                     HYAILEKNRLLLYRGVQNFLEE"
     gene            1684..2616
                     /gene="hpnD"
                     /locus_tag="VN444_01810"
     CDS             1684..2616
                     /gene="hpnD"
                     /locus_tag="VN444_01810"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWR20577.1"
                     /translation="MVEHYNADTSHCRLPPGPDFLIIRFMNQVDDLRTVSRQVTKKSG
                     SNFYYSFLFLPKPKREAIYAVYAFCRLSDDLVDESKVGGDPAAALTRWRKALDTYFQD
                     GVGPPVIMAVGQAAHRFTIPKVYFEELLNGMEMDLTRTRYATFEELYPYCYRVASIVG
                     LICIEIFGYTNPQTKTYAEQLGIAFQLTNIIRDVGVDAQRGRIYLPQDELKRFGYSEE
                     ELLAGRYNRAFMELMRFQCERARGFFQAASAALPVEDERSLLAAEIMRAIYYRLLLRI
                     EAQQYDVFHGNITLGKPQKLLLAAGLWLRSILRA"
BASE COUNT          584 a          801 c          703 g          634 t
ORIGIN      
        1 gtcagatgat aataaacaga tcgtgatcat cgcgtgaggg ggtagccctg cttgagccaa
       61 gcaatggttc ccccttcaag gttgtagagc tgggtcttac caatggccgc cgctacctcg
      121 caggccactg cgctccgaat cccttcggcg cacacgaaga tgatcccgtc ctgctgcagc
      181 agctcacgcg gacggttcat cagcgttcct agagggatat gcacggactc ggcgatatgc
      241 ccatggctcc actccgccgc ctcccgcaca tcgaccacga ccatgccccc tttatcgatc
      301 agccccttgg ccgtctccgg gttgatacgc tcaaatggat ctcgaaactc ttctttcgat
      361 atcatatctt cccttcccaa gcttgacgat gcatcgccgc atcatccgca acaggggaaa
      421 ccgcatgtcc tggcagtgaa tcactacact catcatagcg catcgtccgc ccttgcttca
      481 aggacgatgc gcggcttcac tcctccagga agttctgaac gcctcgatag agcaggaggc
      541 ggttcttctc cagtatggcg taatgggttg aatccccaat gtccaggcgc cgtttggatg
      601 gcgccgatgt gagcttctgc aggagatcct cagcggcctc attgctcatc aactcttcgg
      661 cgctccctcg aacgatcatg gtcggcgcct tgatcctcgc tggatcgaac aacgatcggc
      721 ccgcgtggat ttcgtgccaa tcgatgtacg ggccgttggg aacctggatc gattccgggc
      781 tgcgttgttt ggctgtgggg tcggagctca agagcgtact gatccaagca tcgatgacct
      841 ccctgtctcg ccagtcatcc tggagctccg gcttgatcca cccatcccat tgctcaagga
      901 gcaccttccg agatagatag cgataggccc ccatttcaag atacggtcgg tccgggttgg
      961 tcggatcacc gaacgtactg acaaaggtag aatccttccc gtaaatcgcc ccgtacatca
     1021 ccaacttatt caccttttcc gggtgcatcg tcgcatacag ggcggaccag gaagcgccaa
     1081 tagagtgccc aatgatgttg actctatcca cctttcgctt ggaacggata taatcgacgg
     1141 cgacatcaat atcccgcatc acctgatctg cccgaaccag gggaaggttc tgatccggag
     1201 attccttcat ctccggtggc ctggtcgagc gtccgaatcc cctgatatcc acggtaaaag
     1261 cgtcaaagcc gcgttcagcc acatactcca accaggaata tcctggaagc gcaaggtcaa
     1321 agatgactga gccaggacct ggaagaccgt gaacgaacag gaccacattc tctttggcaa
     1381 tctttggcag gttcgagagc cgcttctctc gaacgaacag tttgaccccc tgatccctgc
     1441 cctgcaccaa aaattcttcc ttgacgatgg cgttctcctg ctccgccgca cctacggcag
     1501 aagcgcttat cgcgacaatc gcgaccgccg tccagcacat cctgccgatt cgcaaccagt
     1561 gatccatcct gcgctccttt ctccgcgaat tatgtcgaag catcaatttc ttctgtattg
     1621 aatgaagtcc cattagtata agactaggag aggcaggagt gtcaacttct tttcatggtg
     1681 cagatggtgg aacactataa cgctgatacc agtcattgcc gtttgcctcc gggacctgat
     1741 tttcttataa tacggtttat gaatcaggtg gatgaccttc ggactgttag tcgccaggtg
     1801 accaaaaaga gcggctcgaa cttctactac tcctttctct tcctcccaaa gccaaaacgc
     1861 gaggcgatct acgccgttta cgccttctgc cgattgagcg acgatctggt ggacgagtca
     1921 aaggttggag gtgatccagc ggccgcgctg acccgatggc gaaaagcgct cgatacctac
     1981 tttcaggacg gcgttggccc ccctgtcatc atggctgttg gccaagccgc gcaccggttc
     2041 actatcccga aggtctactt tgaggagctg ctcaatggca tggagatgga tctcacgcgc
     2101 accagatatg cgacgtttga ggaactctac ccgtactgct atcgcgtggc gtcgattgtg
     2161 ggcctcatct gcatcgaaat ctttggctac accaatccgc agacaaagac gtacgccgaa
     2221 caactgggca tcgcctttca actgaccaac attattcggg atgtgggggt tgatgcacaa
     2281 cgaggacgga tctaccttcc gcaggatgag ttgaagcggt ttggatattc tgaagaagag
     2341 ctgctggccg ggcgatacaa ccgcgccttc atggagttga tgcggtttca gtgtgaacga
     2401 gcccgaggtt tttttcaagc agcctcagcc gcgttgccgg tagaggatga gcggtcgctg
     2461 ctggccgcag agatcatgcg ggccatctac taccgcctgt tgctcaggat cgaagcgcag
     2521 cagtatgatg tcttccacgg gaatatcacc cttggtaaac cccagaagct tttgctggcg
     2581 gccggcctgt ggctccgatc gatactgcgc gcctgatcat cacgctgact ggtgtggtga
     2641 ctctcaaatt cctttacata tccccgttcg ccctgagcct gtcgaagggt gcatattttg
     2701 ggcccttcgg cattgctcag ga
//
LOCUS       DATMXI010000098         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_276712, whole genome shotgun sequence.
ACCESSION   DATMXI010000098 DATMXI010000000
VERSION     DATMXI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276712"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VN444_01815"
     CDS             <1..258
                     /locus_tag="VN444_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWR20578.1"
                     /translation="IQPQSGSEPSALHLHNLIAMIKKNRIKVVVSEVQLSQKIPDLLA
                     KETGARVVVLTTLPGGLPHTETYLDMLRYNVLQLANALETT"
     gene            309..1100
                     /locus_tag="VN444_01820"
     CDS             309..1100
                     /locus_tag="VN444_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWR20579.1"
                     /translation="MAIQPPPFIHFDHATFGFPGVVALEDISLEIQPGEFVGVIGPNG
                     SGKTTLCRAVLGLMAPLKGHLRIFDCACDELRCHHRAKIGYLPQKGVVDRNFPVTVLE
                     TVTMGRYGALGLFKRPGRKDREIAMEALAHVGMDMHKDTALGLLSGGQQQRVFIARAL
                     AQQPQILLLDEPTTGLDITTQHNVVELVQHLHEELGLTVLLITHDINMIRSRVDRLVL
                     LKTRLYAAGPPADVLKPDILSQVYGKDLVITDKDLVIVEDYHHHH"
     gene            1113..1937
                     /locus_tag="VN444_01825"
     CDS             1113..1937
                     /locus_tag="VN444_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWR20580.1"
                     /translation="MLELFAYDFMQRSLLAAALVGALCSTIGVFVVLRGLAFMGAGTA
                     HAAFAGVALGYLMGWPPLLIALLFGLATVWVTGWVEEKGRMKLDVSIGILYTATMALA
                     ILFIGLMKTYNAEVYGYLFGNVLAVTNEELLTIAALSIIVIGTIVLFSKELYFISFDQ
                     EMAEASGVPARRIFFILLTLVALTVVISLKTVGGILVFAMILIPASTAYQLTHSLSTL
                     TWYSIIIGVSCSVGGVLISSYWDIPSGPAIVLLATLLFFIAILLSPKRQRRSLALN"
     gene            2244..2876
                     /locus_tag="VN444_01830"
     CDS             2244..2876
                     /locus_tag="VN444_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWR20581.1"
                     /translation="MSVVMVRWGLVGFMLLSSLALGSHASAEAWKMEQGGIEPGKVVL
                     GMRAGFAPLTQSLTDNTSTEVGSLVNFQGMYSLNKWLLLGMMLEWERHSVNVEQPDVD
                     LGHQDTVSVLPTVEVRPVKFGPVIPYANMSFGVNVNSFGEDVGTTISPSNNFAWRLGW
                     GADYMFTRQFALNTEMAYKRNDGHATIGGARVDDWNASSFGFLFGAKLFF"
BASE COUNT          657 a          903 c          868 g          756 t
ORIGIN      
        1 atccaacccc agtccgggag cgaaccatcg gcgctccatc ttcacaacct catcgcgatg
       61 atcaagaaga accggatcaa ggtcgtggtc tcggaagttc agctcagcca aaagattccc
      121 gacctgttgg ccaaagagac cggcgcgcga gtggtggtgc tcaccacact cccgggaggg
      181 ctcccgcaca cagagaccta tctcgacatg ctccgttata atgtgctcca attggccaac
      241 gcactggaga caacctagct gttgctagag cgcctccagc ggcctgttcc gataggagtc
      301 ttaccgacgt ggccattcag cctcctcctt tcattcactt cgaccatgcc acctttggat
      361 tccccggggt ggttgcgctc gaagatattt cactcgagat tcaacctggc gagttcgtag
      421 gggttattgg acccaacggg tcagggaaga caacactctg ccgcgcagtt ctcgggttga
      481 tggctccact gaaggggcac ctgagaatct tcgattgtgc ctgtgatgag ttgcgctgtc
      541 atcacagggc caagatcggc taccttccac agaagggagt cgtggaccgt aattttcctg
      601 tgacggtgct tgagacagtg acaatgggcc ggtatggggc cttaggcctg ttcaaacgcc
      661 cggggcggaa ggatcgcgag atcgcgatgg aagcactggc tcacgtcggg atggatatgc
      721 acaaggacac agcgctgggc ctgctctccg gaggacaaca gcagcgggtc ttcatcgccc
      781 gagcgttagc gcagcagcca cagatcttgc tacttgacga gccaacgacc ggactggata
      841 tcacaacgca gcacaatgtc gtcgagttgg tgcagcatct gcacgaagaa ttaggtttga
      901 ctgttctctt gatcacccac gacatcaaca tgatccgttc gcgggtagac cggctcgtgc
      961 tcctcaaaac ccgtctctac gccgcaggac cgcctgccga tgtcctgaaa ccggacatcc
     1021 tcagccaggt ctatgggaaa gaccttgtta ttacagacaa agatctcgtc atcgttgagg
     1081 attatcacca ccatcattga tcagaacgta cgatgcttga actattcgcc tacgacttca
     1141 tgcaacgctc gctgctggct gcggcgctgg tcggcgcctt gtgctcgacg attggggtct
     1201 tcgtcgtgct ccgtggactg gcgtttatgg gagccggtac ggcacatgcg gcgtttgccg
     1261 gggttgcgct cggctatctc atgggctggc caccgctcct catagcgctg ctctttggcc
     1321 ttgccaccgt ttgggtcacg ggatgggtcg aagaaaaagg ccgcatgaag ctggatgtct
     1381 ccatcggcat cctctacacc gctaccatgg ccctggccat tctcttcatc ggcctgatga
     1441 agacctataa tgccgaagtc tatggctacc tcttcggaaa cgtgctcgca gtgaccaatg
     1501 aagagctact cacgattgcc gcattaagca tcatcgtcat cggcacaatt gtgctcttct
     1561 cgaaggagct ttacttcatt tcgttcgacc aagagatggc ggaagcctcc ggagtaccgg
     1621 ccagacgcat cttcttcatc ctattgaccc tggtcgcgct caccgtggtg atctctctca
     1681 agaccgtcgg cgggatcctg gtctttgcga tgatcctgat tccagcttcc accgcctacc
     1741 aattgacgca tagtctttcg acgttgacct ggtattccat catcatcgga gtctcctgtt
     1801 cagtcggagg agtcctcatc tcttcttact gggatatccc atccggtcct gccattgtgc
     1861 tgcttgccac gttgctcttc tttatcgcaa tcttgctttc tcccaagaga caacggcggt
     1921 ctcttgccct caattgattt caactccctc tcacgtgtgc tatctgagtc ctcgcacatt
     1981 tcttgaggct cccccctgta gggctacgga tacaccctgt gtcggcaatg agccattaat
     2041 cccgaagtaa aacgggatca aggctgccca tgaatcactc agcaaccata atctggttat
     2101 gatttcttac ttattgagca attttgttta ccagaccaca atcgactcag tggatatccg
     2161 ggcatcgaac ttgctctctt gacgcaggct gaacggaaac ctgtggccaa tttacatcat
     2221 cacctcacgg aggttttatt attatgagtg ttgtcatggt tcggtggggg ttggtaggtt
     2281 tcatgctgct tagctcgctt gccctgggca gccacgcaag cgcagaagcc tggaagatgg
     2341 agcagggcgg gatagaaccg ggtaaggtcg tgctcggtat gcgcgcagga tttgcgccgt
     2401 tgacccaatc gttgactgat aatacctcaa ccgaagtggg ctccttagtc aattttcaag
     2461 ggatgtatag cctcaacaag tggctcttgc tcgggatgat gttggagtgg gaacgccatt
     2521 ccgtaaatgt agaacagccc gacgtggact tgggccatca agacacagtc tctgtgcttc
     2581 cgacggtgga agtccgtccg gtcaaattcg gcccggtcat tccttacgcg aacatgagct
     2641 ttggcgtgaa cgtcaacagc tttggtgaag acgtcggaac taccatttca cccagcaaca
     2701 actttgcctg gcgactcggc tggggcgccg actacatgtt caccagacag ttcgcactga
     2761 acacggaaat ggcctacaag cgcaacgatg gtcacgccac gatcggcggg gctcgtgtgg
     2821 acgactggaa cgcctcgtcc ttcggctttc tcttcggtgc caaactcttc ttttagcagg
     2881 cagttgacaa agtccgccag caggggggat ggcccggtga gtcccctgtg ctcgcgcaac
     2941 gcgcggtcgc ggactcccct cgttggacgc gcgcagtagg ggactcatcc gggccatcct
     3001 taggagaccg taaacgagcg cgcttggaag cacgatcttt ggtcgcttgc tgcggccttg
     3061 cctgcggaat ggcgcgtctt ggcgcgccgg ggttgggcgg gtgagaagaa tagcctgttt
     3121 gagcggcctg cgagaggata gttacaacag acaattagcg cgatcgccgt ttgctctttg
     3181 ccgt
//
LOCUS       DATMXI010000099         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_19866, whole genome shotgun sequence.
ACCESSION   DATMXI010000099 DATMXI010000000
VERSION     DATMXI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19866"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VN444_01835"
     CDS             complement(<1..256)
                     /locus_tag="VN444_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWR20582.1"
                     /translation="MTKFNAMAVVLSLILFQGVAHAVSYERPAIPLPEPRGYVSDHAG
                     AIDGDWKERIRSVCQDLERKTGVEMVVVTVPSIKPFRSANE"
     gene            complement(253..1011)
                     /locus_tag="VN444_01840"
     CDS             complement(253..1011)
                     /locus_tag="VN444_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20583.1"
                     /translation="MAERLIEGLPDDTQKLLQTYVKGVKGQFGELLEGVLLYGSAIRG
                     EFLPGRSNLNILLLVSSYDSAVLKQYSALHRQWSKEQIVAPLFLTEEELRISAEVFPL
                     EFIEIQEQHRVLGGRDPFIGFHVKTDRLKEAVVQGFMSHLLRLRQRYVEGNGTDEATA
                     ILLPLSITSLLPLLRGVQRLLDRPALFLSEAVIKDVAEQLELDLQGLQDILLLKRGQI
                     SPGHHEMPRLFDRYLQTTTLLTRAVVQLSQPGQR"
     gene            complement(1024..2298)
                     /locus_tag="VN444_01845"
     CDS             complement(1024..2298)
                     /locus_tag="VN444_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWR20584.1"
                     /translation="MTEETDVAVVGAGGGGAVLALALAKQGVSTMVLDQAVGPPKGLR
                     GEILQPNGQQILDRLGVLPSLPTDATRSVRHFHFCRAGGTRLCSIDYGDLPAPYNRAI
                     VTLPNVAHHAIVAAVQQQKSVTLRYGTSFTGLVREGPRVVGLSTTGPDSIRTIRAKIV
                     VGADGAFSKVREALKIPADLYLYPDGYLIAILESEDPVSEAFYYIGERAILGLFPATG
                     NKVYIFYMIPKDSMEAVKQRGIPALQQAWSQIAPQFAGLFRHLNDWTQTAYMPTGRVR
                     TPTWVADGAVLIGDAAHAMNPHASQGRMQAMVDAMALSDLLPGCLVDRDYSAARLSQF
                     ERARRPQVTMLQRLADQQVFYWNTANPVVAFLRDRVFQTLDRNARLRYQVLSTTAGLR
                     TVPPFNMIDRVIAAGFLPDVRSNERSPHPMSL"
     gene            complement(2417..>2915)
                     /locus_tag="VN444_01850"
     CDS             complement(2417..>2915)
                     /locus_tag="VN444_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWR20585.1"
                     /translation="MLIAACPVGAGWSATVEVYYGPDDAPLDRLTALYRRATRYLYVA
                     VYGLTSPRAVAAMVAAKKRGVDVRMITDRQRTEDVKQRTALTTLHLAGIPILVNEHDG
                     LMHLKQVVIDDEVNTTGSMNHTTSGNSYNDERLDVITDPAITVRAREKFLAMWNDHTR
                     FKEWK"
BASE COUNT          647 a          878 c          752 g          638 t
ORIGIN      
        1 actcattggc cgaccggaat ggcttgatac tcggcaccgt gaccaccacc atctcgacgc
       61 cggtctttcg ttcaagatcc tgacagacgg aacgaatgcg ctctttccaa tccccatcga
      121 tggcccctgc gtgatcgctc acataccccc gtggttcagg caacggaatc gcgggccgtt
      181 cgtaggacac cgcatgggct acgccctgga acagaataag ggaaagtacc acagccatag
      241 cattgaactt tgtcatcgct gtcccggctg cgatagttgt acgactgcgc gagtcagcaa
      301 cgtcgtggtc tgtagatacc ggtcgaagag ccgaggcatt tcatggtggc ccggagatat
      361 ctggccgcgt ttgagcaata ggatatcctg caatccctgc agatccagct ccaactgctc
      421 agcgacgtcc ttgataactg cctctgagag aaagagggcc ggtcgatcga gaagacgctg
      481 cacgccgcgc agcagtggaa gaagtgacgt gatcgagagg ggcagtaaaa ttgccgtcgc
      541 ctcgtcagtc ccgttccctt cgacataccg ctggcgtaga cgcaagagat ggctcatgaa
      601 accctggacc acggcctctt tcagccgatc ggtcttcacg tggaatccaa tgaagggatc
      661 acgcccgcct aagacgcgat gctgttcctg aatttctata aactcaaggg gaaatacctc
      721 tgcagatatg cgcagctcct cttccgtcag aaatagggga gccacaatct gttccttgct
      781 ccactgacga tgcaatgcgc tgtactgttt aaggactgcg ctgtcatatg acgacaccag
      841 gaggaggata ttcaaattgg atcgccctgg gagaaattca ccccggatag cactgccata
      901 gagcaataca ccttccaaca actccccgaa ctggcctttg acccccttca cgtaggtctg
      961 cagcagcttc tgtgtgtcgt cgggcaaccc ctcgatcaga cgttcagcca ttccaatggt
     1021 tcttcacaac gacatcggat gtggagatcg ctcattcgaa cgcacatcgg gaagaaaccc
     1081 cgcagcgatc acgcgatcga tcatattaaa aggcggtacg gttcgtagcc ctgccgtggt
     1141 cgacagaacc tgatagcgca gccgcgcatt tcgatccaga gtttgaaata cgcgatcacg
     1201 caaaaaggcc acaacaggat ttgccgtatt ccaataaaat acctgttgat cagccaatcg
     1261 ctgcagcatt gtcacttgcg gccgcctcgc tcgttcaaac tggctcagcc tggctgctga
     1321 atagtctctg tcaacgagac agcccggcaa caaatcggat agtgccatgg catccaccat
     1381 cgcctgcata cgcccctgtg acgcatgggg attcatcgca tgggcggcat caccaatcag
     1441 cacggcacca tctgcgaccc atgttggcgt acgcacccgc cctgtcggca tataggcggt
     1501 ctgcgtccaa tcgttcaggt ggcgaaatag ccctgcaaac tgcggcgcga tctggctcca
     1561 tgcctgctgt aatgcaggga tcccccgttg cttcaccgcc tccatcgaat ccttgggaat
     1621 catgtagaaa atatagacct tgtttcctgt cgctgggaag agtcctaaga tcgctctctc
     1681 cccgatgtag tagaatgctt ccgagaccgg gtcctccgac tcgagaatgg caatgagata
     1741 gccatccggg tatagataca gatccgccgg gattttcagt gcctcgcgaa cctttgaaaa
     1801 tgctccatcg gctccgacga cgatcttcgc acggatcgtg cggatactgt cgggccctgt
     1861 cgtagacaga ccgactacgc gagggccctc ccgcacgagc cctgtaaatg atgttccata
     1921 acggagcgtg acgctctttt gctgctgaac cgccgcaaca atggcatggt gagctacatt
     1981 cggaagcgtc acgatggcac ggttgtacgg cgccgggaga tcaccatagt cgatactgca
     2041 gaggcgtgtc ccccctgctc gacaaaaatg gaagtgccgt acggatctcg tggcatcggt
     2101 cggcaatgat ggcaagacgc ctaaacggtc gagtatctgc tgcccgttag gttggagaat
     2161 ctctccccgc aggcccttcg gcggtccgac agcctggtcc aacaccatgg tgctgacccc
     2221 ttgtttggcc agcgccaggg ccaagacggc gccgcctcct cccgccccta cgactgcaac
     2281 atccgtctcc tcagtcatcg ctcctcatac agaaaggttt aggttgaggt cgaggttgag
     2341 cctgaaggta caagagagtt agcagaactc tcctctttct cgaccttaac cttaacctta
     2401 gcctcaacct tcttgctcat ttccattctt taaatctcgt gtgatcgttc cacattgcca
     2461 ggaacttctc ccgggccctg accgtaatag cagggtcggt aatgacatcg agccgttcat
     2521 cgttataact attgccgctc gtcgtgtggt tcatcgaccc cgtcgtgttc acttcgtcat
     2581 cgatcacaac ctgcttcaga tgcatgagcc catcgtgctc gtttacgaga ataggaattc
     2641 ccgccagatg cagcgtggtg agtgctgtgc gctgtttgac atcttctgtg cgctggcgat
     2701 cggtgatcat tcgcacatcg actccccgtt tctttgctgc aaccattgcc gccactgcac
     2761 ggggagaggt cagcccatac accgccacat agagatagcg tgtcgcccgc cggtagagtg
     2821 ccgtcagacg atccaacggc gcgtcgtccg gcccataata gacttccacc gtcgcgctcc
     2881 agccggcccc gactggacac gcagctatca gcatg
//
LOCUS       DATMXI010000100         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_41176, whole genome shotgun sequence.
ACCESSION   DATMXI010000100 DATMXI010000000
VERSION     DATMXI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41176"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VN444_01855"
     CDS             complement(<1..708)
                     /locus_tag="VN444_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20586.1"
                     /translation="MRLEVGRSAGPEWTRWVERFEGNIYHSQEWAEICRSEHVYPLFF
                     RGLDEQGRCVGVALGIERRSSRPVIGHFSRRLDFETFPAVQGNDVQLASRMISLLVEF
                     AKAGGYRGLTIQSHYAGMMVADLDRLGLMITPRVECIVDLTRSEEERWKQLSTHHQRK
                     IKKAGRHGLVLEEVCTAEAMQELRRLQIGSRDRRTARGEYFGLQDEAYYEQMGRRYFE
                     RNLGRLFIMRHEGHAVSA"
     gene            complement(698..2098)
                     /locus_tag="VN444_01860"
     CDS             complement(698..2098)
                     /locus_tag="VN444_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20587.1"
                     /translation="MGKKVLLISYHYPPSVEVGGLRCANFSRHLPRFGWIPYVLTLKD
                     EYLDTADPEKLKCVGSVKIFKAGRMPTVSQAYVGIKRIAHWLFRKSSESVTRSDGGTG
                     PGQSSSRVAETWSQKLRRYILSFLSLPDEQRNWVLPAVIQGVRIIKREKIDCIFTSSP
                     PYSVNLVGWLLKAITGVRWIADFRDPWMTGGSKKLYVTCAVSLGIERWLERRVVEKAD
                     LLVANTEMLSEAFRKAYGSQLSDRCVCITNGFDGELFSSFGPVEKEKVFTITYTGTLY
                     FGRTPEPVFQAVHELIHEKRIDAQSIRIRLIGHCQVIDGRPTGEIVHDYQLEGIVEVL
                     KPVSYKRAIEMVKQSQLALLLAPNQPYQIPAKAYDYMGTGTRILALTEDGATCDLVRS
                     TGVGAAFRPSDTAGIKEFLLRSMSPDCLSIDSKSKGPVSEYEIQSLTRRLAQYLDSFG
                     GQAERAERVGGSGHAA"
     gene            complement(2105..3169)
                     /locus_tag="VN444_01865"
     CDS             complement(2105..3169)
                     /locus_tag="VN444_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20588.1"
                     /translation="MGRRLLRVERLAARIRKIWEEKRNSAGQTYVHHRVSEYQKIWRN
                     VAAKVGARFTVLADSVWELELNGKTTRILNDKLEFDNPVTLEIAGMKPLVYQLFAERG
                     LRVPEHRTYQLDDWKQAAEFLKSHPKGCVVKPANGTSSGQGVTTHILTESEVKTASIL
                     ASLYCSDLLIEPMIPGECYRLLVLDGELVHAVRRTGPRLVGDGVSTIASLLQVDNEFR
                     RSRGEQPLDADRDCLFTLDYQGLSLESVPLHGQAVLVKSVNDPRRKCVEVRTVYNDVV
                     THLICDSLRHNAEAAAKILNSRLVGVDFITVDPTVPLDKSGGVINEVNTTPGLHHHYD
                     AAWESFPEPAPRILQSLFSR"
BASE COUNT          719 a          985 c          815 g          735 t
ORIGIN      
        1 ggccgaaaca gcgtgccctt catgccgcat gatgaacagg cggcctagat ttctttcaaa
       61 gtaccgccga cccatctgtt cgtaataggc ttcgtcctgc aagccgaaat actcgccccg
      121 tgccgttctt cggtcgcgcg aaccgatctg gagcctcctc aactcctgca tagcctcggc
      181 tgtgcagacc tcctccaaga ccagaccatg ccggccggct tttttgatct tcctctgatg
      241 gtgggtcgaa agctgcttcc accgctcctc ttcagacctc gtcagatcga caatgcactc
      301 aacccttggt gtgatcatca gacccaggcg gtccagatcg gcaaccatca tcccggcata
      361 gtgagactgg atcgtgagtc cacgatatcc tccggccttc gcaaactcga ccaacaggct
      421 gatcatccgg ctggctagtt ggacgtcatt cccctgaacc gcggggaatg tctcgaaatc
      481 cagtctcctt gaaaaatgcc cgatcaccgg ccttgaagag cggcgctcga ttccaagcgc
      541 gaccccgacg caccggccct gttcatccag tcctcggaaa aacaatgggt agacatgctc
      601 cgatcgacaa atctcggccc actcctggct gtgatagata ttgccctcga accgctcaac
      661 ccatcgggtc cattccggtc cggctgacct tccgacttca agccgcatgt cctgatcctc
      721 ctacccgttc agcgcgttcc gcctggccgc cgaagctatc cagatattgc gcgagacgtc
      781 gtgtcagaga ttgaatctcg tactcggaga ctggaccttt gctcttggag tcgatcgaca
      841 agcagtctgg actcatggat cgaagcagaa actccttgat ccctgccgta tcggaagggc
      901 gaaacgccgc tccaaccccg gtagagcgaa ctaggtcgca cgtcgctcca tcttctgtca
      961 aagccaggat tctggtccca gttcccatat agtcgtaggc tttcgctgga atctgatacg
     1021 gctggttcgg cgctaagagc agcgcgagtt ggctttgctt aaccatctcg atcgcccgct
     1081 tatatgaaac cggtttcagg acctccacaa tcccttctaa ctgatagtcg tgcacgatct
     1141 cccctgtggg cctgccatca atgacctgac aatgccctat caatcgaatc cggatcgatt
     1201 gggcatcaat ccgtttctca tggatcagct catgaacagc ctgaaacacc ggctctggtg
     1261 tccttccgaa atacagggtc ccggtatagg tgatcgtaaa gaccttttcc ttctccacag
     1321 ggccgaaact tgagaacaat tccccatcga atccgttcgt gatacagaca caccggtcag
     1381 acagctgaga gccatacgcc tttctgaagg cctcggataa catctcggta ttcgccacga
     1441 gcaaatcggc tttctcaacc actctccgtt ccagccatcg ctcgattccc aacgacaccg
     1501 cacaggtcac gtacagtttc ttggaacccc cggtcatcca tggatccctg aaatcggcaa
     1561 tccagcgcac acccgtaata gccttgagca accatccgac cagattgacc gaatacggtg
     1621 ggctcgacgt gaagatacag tcgatcttct ctcgtttgat aatacgaacg ccttgaatca
     1681 cagcaggcaa gacccagttc cgctgctcgt ccggaagcga caaaaaagat aggatgtatc
     1741 gacggagttt ctgagaccag gtctcggcga cgcgactgga tgattgacca ggtccagtac
     1801 cgccgtctga tcgagtgacc gattcacttg attttctgaa cagccagtgc gctatgcgtt
     1861 tgattcccac ataggcttgt gagactgtgg gcatcctgcc agccttgaaa atcttgacgg
     1921 agccaacgca cttcagcttc tcaggatctg ctgtatcaag atattcgtct ttcaaggtca
     1981 ggacgtaggg aatccatccg aagcggggca ggtgcctgga aaagtttgca catcgcagac
     2041 cacccacctc cacgctgggc ggatagtgat aggaaatgag cagtaccttc ttgcccatgg
     2101 acggttaccg actgaacaac gattggagta tccgcggggc aggctcggga aaagattccc
     2161 aggccgcgtc atagtggtga tgcagcccgg gtgtcgtatt gacctcgttg atcacccctc
     2221 cgctcttgtc caaaggaacc gtgggatcga ccgtgatgaa atccaccccg accaggcgcg
     2281 agttcagaat cttggccgct gcctctgcat tatgtctcaa cgagtcgcag atgagatgag
     2341 tcacgacatc gttgtagacg gtccgaacct cgacgcactt cctccgggga tcgttcacgc
     2401 ttttgacgag gacggcctgt ccatggaggg gcaccgattc caatgagagc ccttgataat
     2461 cgagggtgaa gaggcaatcg cgatcggcat caaggggctg ctcacctcga ctcctccgga
     2521 attcattgtc cacctgaagc aggctggcta tcgttgagac tccatccccg accagtcttg
     2581 gccctgtccg acggacagca tggaccaatt cgccatctaa gaccaggaga cggtagcact
     2641 ctccggggat catcggctca atcaagagat ccgagcaata gagagacgct agaatggagg
     2701 ctgtcttcac ctcactctcc gtcaaaatat gggtcgtgac accttgaccg gacgaggttc
     2761 cattcgcggg tttgacgaca caccctttcg gatggctttt gagaaattcg gcggcttgtt
     2821 tccagtcgtc gagttggtat gtccgatgct ccggcacccg gagtccacgt tcggcgaata
     2881 gctggtagac caacggcttc atgccggcga tctcgagcgt aaccggatta tcgaactcca
     2941 gcttgtcgtt gagaatccgt gttgtctttc cattcagctc cagttcccaa accgaatccg
     3001 ccaagactgt aaagcgagct ccgaccttcg ctgccacatt tctccagatc ttctgatatt
     3061 ctgaaacgcg gtgatggaca tacgtttgcc cggcagaatt ccgtttctcc tcccagatct
     3121 tgcggatcct ggcggctagt cgttctactc tcagcaggcg tcgtcccatc ttcacctctc
     3181 aatcaccaga ggcctgatct tggccaagcg gcgcgtcgag gcattgcagt gagccgcacg
     3241 ggtagatccg gacc
//
LOCUS       DATMXI010000101         6561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_55462, whole genome shotgun sequence.
ACCESSION   DATMXI010000101 DATMXI010000000
VERSION     DATMXI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55462"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VN444_01870"
     CDS             <1..755
                     /locus_tag="VN444_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWR20589.1"
                     /translation="LERLDSEAIMIVLPADHYVPDGEAFAAGVRRATELLASYPDWVV
                     MFGIPPTRPETGYGYLEVGELLAGSLSAFRIRQFLEKPDRQTAECLATDGCHYWNSGI
                     FLWRNATIQSLLARHMPEVWAGLCRIREAWGVQEALVREYSALKKQSVDYGVLERMEE
                     GVAMVRADFAWDDLGTWDALARVLPVDDDGNVIVGEVQSLDTTQSVIVSAGPRVATVG
                     LSEMIVVASKDGVLVCPKGRAQEVRRLAGESR"
     gene            752..1726
                     /gene="gmd"
                     /locus_tag="VN444_01875"
     CDS             752..1726
                     /gene="gmd"
                     /locus_tag="VN444_01875"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWR20590.1"
                     /translation="MTARRALITGITGQDGSYLAEFLLQRGYTVAGMVRWSSTEPSDR
                     IAHMLHRITLQQADLLDQASLIHLIREVRPHEIYNLASQSFVPTSWEQAVLTAEFTAV
                     GVTRLLEAVRLVDPGIRVYQASTSEMFGKAQEVPQHERTPFYPRSPYGVAKLYGHWIT
                     VNYRENYGLFACSGILFNHESPRRAKEFVSRKITDGVARIKRGLATELKLGNLEARRD
                     WGYAGDYVKAMWLMLQQESPDDYVIATGETHSVRELVQIAFARAGLDWREHVVEDPAL
                     KRPAEVDLLQGDASKAKRVLGWQPNVGFRELIEMMVDADLERYAEALS"
     gene            complement(1813..2724)
                     /locus_tag="VN444_01880"
     CDS             complement(1813..2724)
                     /locus_tag="VN444_01880"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWR20591.1"
                     /translation="MTYAYFKGQFVPLDQAKVSIQNNTFQYGTGIFEGIRAYWNPDEG
                     QLYLFRMVEHYIRLLRNCRVLKLTISKDERELSEITLELLRKNHPETDTYIRPIAYVD
                     SDGIGPKFIGYSTGFAMYTLPLGDYIDVSRGIKVGFSSWRRINDNAIPARCKVTGGYV
                     NSALAKTEALEHGYDEAIFLTEGGFISEGSAENIFLIRGGKLITPALSEDILEGITRE
                     TVIELAREELGIETIERPIGRTELYVADEAFLCGTGAQISPMIEVDKRPLGTGKIGPI
                     SAKIQALYFDVVKGKQKKYAHWLTPVY"
     gene            2929..3315
                     /locus_tag="VN444_01885"
     CDS             2929..3315
                     /locus_tag="VN444_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWR20592.1"
                     /translation="MVRKATVQVILLTALVVGCTGAWTAVPATAEPKAPEKFMFCAPC
                     HGPAGEGNGPAAAALNPKPRNFTDGKYMNVRTDAQVINVIKNGSASEKFSPLMIGFGN
                     LLNDMEIKDIVAYIRSLAVPKYQPKK"
     gene            complement(3427..4626)
                     /gene="thiI"
                     /locus_tag="VN444_01890"
     CDS             complement(3427..4626)
                     /gene="thiI"
                     /locus_tag="VN444_01890"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HWR20593.1"
                     /translation="MKSGGNAIARSPAKGALIHYHEIALKGKNRGFFLRQLEANLLLA
                     TRDLDCGPLRRPAGRLFLQMREETSWEILRQRLSRVFGIANFAPAFTMAPNLELLAKR
                     IEEEVSGRSFRSFRVAARRAFKTFPQTSQEINEIIGARVQRICGASVDLTNPDLTIYI
                     ELLPHEAFVYFEKVSGPGGLPVGTGGTVVCLLSGGIDSPVAAYRMMKRGCRVVFVHFH
                     SQPFADRTSQEKAIELVRLLTRYQFASCLHLVPFGEIQQEVVSHVTGRLRVLVYRRLM
                     LRIAEQIAQKEGAQALVTGESLGQVASQTIENIATIEQASSLPILRPLIGMDKDEITQ
                     QAQQIGSYDVSVIPDQDCCSMFLPRQVATHTTHREIEFAERPLDLDRLVTQAHSVAHS
                     LELSFPV"
     gene            4968..5645
                     /locus_tag="VN444_01895"
     CDS             4968..5645
                     /locus_tag="VN444_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR20594.1"
                     /translation="MPRIARIVAPGHPYHVTQRGNYRQAVFRNDEDRSQYLSWVNEYS
                     RKFHLSIWAYCLMDNHVHFIVQPQEEASLAKVFSVAHMRYAQYFNKERKASGHLWQGR
                     FYSCLLDEPYLMAAIRYVERNPVRSRMVNKPWQWKWSSAAAHIGGTDEMIDVIEIKES
                     LGVSMESWREHIDSDDNAPDVEAIRRHTMTGRPLGRDGFIGELSRKLGRVISVLRRGR
                     PRKDLNK"
     gene            6036..>6561
                     /locus_tag="VN444_01900"
     CDS             6036..>6561
                     /locus_tag="VN444_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20595.1"
                     /translation="MKKPLSIATGEGAGADRRCPTPSIEERSTLPLIIQGGMGAAVSD
                     WRLANAVARTGQMGVVSGTGIDTVFVRRLQDGDCGGHMRRGIEQFPIRRAADEAIRRY
                     YRPEGRAPGEPYTMLPMYRQVVAPARQELTMLATFVEVFLAKEGHDAPVGINLLTKVQ
                     MLTLPTLYGAMLAGV"
BASE COUNT         1375 a         1779 c         2019 g         1388 t
ORIGIN      
        1 atctcgagcg gcttgactca gaagcgataa tgattgtctt accggctgat cactacgtcc
       61 cggacggcga ggcgtttgct gcaggagtgc gccgggcgac cgagctgctc gcctcgtacc
      121 cggactgggt cgtgatgttc gggatccccc ctacccggcc agagaccggg tacgggtatt
      181 tggaggtcgg tgagctgctt gccgggtctc ttagcgcctt ccgcatccgt caatttctgg
      241 agaaaccaga tcgacagacg gccgagtgtt tggcaacaga cgggtgccac tactggaaca
      301 gcggcatctt tctttggcgg aatgccacca ttcaaagtct gctcgccagg catatgccgg
      361 aagtgtgggc gggcctttgt cgtatccgcg aggcgtgggg ggtgcaggag gcgctggtcc
      421 gggagtacag cgcgcttaag aagcagtcgg tggactacgg cgtgctggag cggatggagg
      481 agggggttgc gatggtcagg gccgatttcg cctgggatga tctggggacc tgggatgcgt
      541 tggctcgagt gctgcctgtg gacgatgacg gcaatgtgat cgtgggtgag gtgcagtccc
      601 tggacacgac gcagtcggtc attgtctcgg ccgggccgcg ggtggcaacc gttgggctgt
      661 cggagatgat tgtggtagcg tctaaggacg gcgtactggt ctgtcccaag gggcgggccc
      721 aggaggtcag aaggcttgcg ggggagtccc gatgactgcg cggcgggcgt taattactgg
      781 cattacgggc caggacggct cctatctggc cgagtttctt ctccagcgag gatacacggt
      841 ggccgggatg gtacggtggt cgagtaccga gccgtccgac cggatcgcgc acatgcttca
      901 taggatcacc ctgcagcagg cggacctcct tgaccaggcg tctctgattc atctcatccg
      961 ggaggtccgg ccgcacgaga tctacaacct cgcttcgcag tcgttcgtcc cgacctcctg
     1021 ggagcaggcg gtcctgacgg cggagttcac cgccgtgggg gtgacccgcc tactggaggc
     1081 agtccgcctt gtggatcctg gcatacgggt ctaccaggcc agcaccagcg agatgttcgg
     1141 caaggcccag gaggtcccgc aacacgagcg gacgccgttc tatccgagaa gcccatacgg
     1201 tgtggccaag ctctacggcc actggatcac ggtcaactac cgggagaact acgggttgtt
     1261 cgcctgttct ggcattctgt tcaaccacga gtcccctcgc cgggccaagg aatttgtgag
     1321 ccgaaagatc accgatggcg tcgcgcgtat taagcgcggc ttggccacgg agctgaagct
     1381 gggaaacctg gaggcgcggc gggattgggg gtatgccggt gactatgtga aggcgatgtg
     1441 gttgatgctg caacaggaga gcccggatga ctacgtcatc gccacgggcg agacccattc
     1501 agtccgggag ctcgtccaga tcgcgttcgc gagagcaggg ctcgattggc gggagcatgt
     1561 ggttgaagat cctgcgctga aacgacccgc tgaggtcgat ctgctgcagg gggacgcatc
     1621 caaggcgaag cgcgtgttag gctggcaacc gaatgtcggc tttcgtgagt tgatcgagat
     1681 gatggtagat gccgatctgg agcgttacgc cgaggcctta tcctgatctg ggcatagcgg
     1741 ggaggtaggg ctggtggaga cctgcattgt ggggacgggg taagtcggcc tcgttacctg
     1801 ggcgtgggct ttctaataga ctggcgttag ccagtgcgcg tacttcttct gctttccctt
     1861 gaccacgtcg aaatagagcg cttgaatctt cgcgctgatc ggccctatct taccggtacc
     1921 aagcggtcgt ttatccacct caatcatcgg cgagatctgg gcgcccgtcc cgcagagaaa
     1981 cgcctcatcg gcgacatata gctccgtcct gccgattggc cgctcgatcg tctcgatgcc
     2041 cagctcctcc cgagctagct cgatcactgt ctcgcgcgtg atcccttcca ggatatcctc
     2101 ggacagggca ggcgtgatca actttccccc cctgatgaga aagatgttct ccgccgatcc
     2161 ttcagagata aagcccccct cggtgagaaa gatcgcctca tcgtagccat gttcgagggc
     2221 ctcggtcttg gccagtgcgg agttgacgta gccgcccgtg accttgcagc gggccggaat
     2281 ggcgttatcg ttgatccggc gccaggaaga aaagcccacc ttgatccccc ttgagacgtc
     2341 gatgtagtcg ccaagcggca gcgtgtacat ggcgaaaccg gtggaatagc caatgaactt
     2401 cgggccgatc ccatcagaat cgacatatgc aatcggccgg atataggtat cggtttcggg
     2461 gtgatttttc ctgagcaatt cgagggtgat ttccgacaac tccctctcgt ccttactgat
     2521 cgtgagcttc aggactcggc agttcctgag cagtcgaatg tagtgttcca ccatgcggaa
     2581 caggtagagc tgcccctcat ccgggttcca gtaggcgcgg ataccctcga agatgccggt
     2641 cccgtactgg aaggtattgt tctggatgct gaccttggct tgatcaagcg gaacgaactg
     2701 ccccttgaaa taggcgtacg tcatcgtgcc cctctctccg caccaccaaa caatacccgc
     2761 ataccgggct caacctagcc ggaatcaaat gaaaagtcaa caggttttgg gcttctcaag
     2821 gacaggtcaa cttcctcttg acatgggaaa atctagagcg cacaatttaa caagatgtta
     2881 gtttcttatt gtatgtgatg agcgggaacc actttacagg aggaatcgat ggtgagaaaa
     2941 gcgaccgttc aagtgatctt gctaactgct ttggtcgtgg gctgcacagg agcctggaca
     3001 gccgtgccgg ctaccgccga gcccaaggct ccagagaagt ttatgttctg tgctccgtgt
     3061 cacggtccag cgggtgaagg gaatgggccg gcagcggctg ctctgaatcc caagccacgg
     3121 aactttacgg acggcaagta tatgaatgtc agaaccgatg cgcaagtgat taatgtcatc
     3181 aagaatggca gtgcgtcaga gaagttcagc ccgctgatga tcggctttgg gaatttactc
     3241 aatgacatgg agatcaagga catcgtcgcc tatattcgct cactggccgt cccgaagtac
     3301 cagccgaaga agtgaagcac agctcttgag cgatcagccg tcagcaaaat gatgggtcgt
     3361 taaaggcgat ccgcaattct ggaagcgaag gcgctgaccg atgattgctg aaagccgaac
     3421 cgctgttcaa acgggaaacg acagttcgag ggagtgcgca acagagtgcg cctgcgtcac
     3481 gagtcggtcc aggtcaaggg ggcgctcggc gaattcgatc tcgcggtgag tcgtgtgcgt
     3541 cgccacctgc cttggcagaa acattgagca gcagtcttgg tcggggatga cagagacatc
     3601 gtagctacca atctgctgcg cctgctgcgt gatctcgtcc ttgtccattc cgatcaaggg
     3661 ccgcagaatc ggcagagagg atgcctgctc gatggtggcg atgttttcaa tggtttgtga
     3721 cgccacctga ccgagactct cgccggtcac cagggcttgg gcgccctcct tttgggcgat
     3781 ctgttcagca atccgcagca tcagccgtcg atacacaagg accctcaggc gccccgtgac
     3841 gtgagagact acctcctgct gaatttcgcc gaagggcacc aggtggaggc aagaggcaaa
     3901 ctggtaccgg gtgaggagtc gcaccagttc gatcgccttt tcttgtgagg ttcggtcggc
     3961 gaatggctgg ctatggaagt ggacaaagac gacacggcag ccccgcttca tcatccggta
     4021 ggccgccacc ggcgaatcga tcccgcccga tagcagacag acgaccgttc cgccggtccc
     4081 caccggcaag cccccagggc ccgagacctt ctcgaaatag acaaacgctt cgtgggggag
     4141 tagttcgatg tagatggtca ggtccggatt ggtgagatcg acgctggcgc cacagatgcg
     4201 ctgcaccctg gcccctatta tctcgttgat ttcctgggag gtctggggaa aggtcttgaa
     4261 ggcacgacgg gccgctacgc gaaagctgcg aaacgaacgg cccgagacct cctcttcgat
     4321 ccgtttcgcg agcaactcca ggttgggtgc catcgtgaag gcgggggcga agttcgcaat
     4381 cccgaagact cggctcagcc gctgtcgcag gatctcccac gaggtctctt cgcgcatctg
     4441 taggaacagc cgaccggcag gcctccggag cgggccgcaa tcgagatcgc gcgtggctag
     4501 caggagattg gcctcaagct gcctcagaaa gaagccccgg tttttcccct tgagggcgat
     4561 ctcgtggtaa tggatgaggg cgcccttcgc cggtgatcgt gctatcgcgt tgcctccact
     4621 cttcacaatg cctcctggcc tcccccatcc taatgtgtag tgtctcgcaa atatctttac
     4681 aatccgttcg tggtgagcag ttcgacagac tcaccacagg cttgccgaac catgaacgaa
     4741 acttgtcgaa atccttctca cctttcgaca agctcagggc gaacggaacg tggaaagaag
     4801 cgttggacac actacactag cacgttctcc acgatcggca atcgatttcc gctcgcatgc
     4861 aaaggactca cgcgaggttt atttaaatag ggacattaaa taggacatta aatagggaca
     4921 gacactattt atttgacaat ttgtggtgtt tgctatagtg atcttgcatg ccacggattg
     4981 ctcggatcgt tgcgccaggc cacccttacc atgttacgca aagagggaat tatcgccagg
     5041 cggtctttcg gaacgacgaa gatcgttctc aatatttatc ctgggtgaat gaatacagcc
     5101 gcaaatttca cctgtcgata tgggcctatt gcttaatgga caaccatgtc catttcattg
     5161 tccagccgca ggaagaggca tcgttggcta aagtattcag cgtggcacat atgcggtatg
     5221 ctcaatattt caacaaagag aggaaggcct ccgggcactt gtggcaggga cgcttttatt
     5281 cctgtctgct tgatgagcct tatcttatgg cggcaatacg gtatgttgag aggaatccgg
     5341 tgcggtccag gatggtcaac aagccttggc agtggaaatg gtccagtgcg gccgctcaca
     5401 tcggtggaac ggatgagatg atcgatgtca ttgagataaa ggagtcactg ggtgtttcca
     5461 tggagtcatg gagagaacac attgactcgg acgataatgc cccagacgtg gaagcgattc
     5521 gaaggcacac gatgacaggg cgaccactgg ggagggatgg gttcatcgga gaattgagta
     5581 ggaagctggg gcgagtaata agcgttttgc ggagaggaag accaagaaag gatctcaata
     5641 aatagtgtct gtccctattt cctatttcaa ctctgataca cttttcatga ccatttgtgg
     5701 tacatttcct tgtgaccggt gacacacata cgtaatccgt atgtgcggta gtgtgggagg
     5761 agaggggccg cgaggcctat ccctattccg actgcggacg tagaaaacga ttcctgggca
     5821 agatgcataa aacagtgacc tcctagaatg cgccataatc aacgatctca tgcctgggaa
     5881 gggtgttcgg caatagtagg cggtggtgcg cggagcaggc tggtggccgg ctcaaatcgc
     5941 acgcgattca ctgcagcatg ccgggcttgc gatgcggatc gagccgagcc ccaccactcg
     6001 ccctcgcgta gttgcgtaac cggtcgcgtg gcggtatgaa gaagccctta tcgatcgcaa
     6061 ccggtgaagg cgcgggagcg gatcgtcgat gccctactcc ttccatcgaa gagcgctcaa
     6121 cgcttccact gatcattcaa ggcggtatgg gtgccgctgt ttcagactgg cggctggcca
     6181 acgccgtggc acgaactggc cagatggggg tcgtctccgg caccggcatc gatacggtgt
     6241 tcgtacggcg cctgcaggat ggcgattgcg gcggccacat gcgccgaggg atcgagcagt
     6301 tcccgatccg tcgcgccgca gacgaggcca tacgccgcta ctatcggcca gagggtcggg
     6361 cgcctggcga gccgtacacg atgctcccca tgtaccgcca ggtggtcgct cccgctcggc
     6421 aggagctcac catgctggcc acattcgtcg aagtgttcct cgccaaggag ggacacgatg
     6481 caccggtcgg catcaacctg ctcaccaaag ttcagatgct cacgctgccg acgctctacg
     6541 gcgcgatgct cgccggggtc g
//
LOCUS       DATMXI010000102         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_145802, whole genome shotgun sequence.
ACCESSION   DATMXI010000102 DATMXI010000000
VERSION     DATMXI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145802"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VN444_01905"
     CDS             complement(<1..651)
                     /locus_tag="VN444_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWR20596.1"
                     /translation="MNYKETLNLPNTAFPMKADLPVLEPSILKRWDASGLYDRLREVR
                     ADRQVWILHDGPPYANGHIHIGHALNKILKDFVVKSKSMFGYNATYVPGWDCHGLPIE
                     HQVDKNLGSKKTEVSLVEKRQLCREYAAGFVDIQREEFRRLGVLGNWSAPYLTMDYMY
                     EAAIVRELGRLFGTGAVYKGKKPVHWCASCRTALAEAEVEYADHTSPSIYVKFPLTP"
     gene            complement(776..1876)
                     /gene="rodA"
                     /locus_tag="VN444_01910"
     CDS             complement(776..1876)
                     /gene="rodA"
                     /locus_tag="VN444_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWR20597.1"
                     /translation="MLIDRRALPAFDWRLAASAAGLAALSVLIIYSTSGLHSSLFRRS
                     LYLKQATWAVMGLFAMILLCGLHYRTIWRLAYPLYGVILATLLLTALIGRAGMGAQRW
                     LSIGSFAFQPSEFMKLSLILLLARYFEDHKDDLRAPRIFLPPILLTLLPAAFVLRQPD
                     LGTAILLLFTSASILVLMGLKIRYFVMLGTLGAVITPVLWRFLHDYQKSRLLVFIDPG
                     MDPMGAGYHLAQSKIAVGSGGLLGKGWMAATQSQLNFLPANHTDFIFAGFAEQWGFIG
                     SLGLLLLYAYLFSKGLRLAKDARDLFTMVTSFGIVSMMALQVVINVGMVVGIIPVVGV
                     PLPLLSYGGSAMLVNMLAVGLLLNIQKHRYLY"
     gene            complement(1893..>2804)
                     /locus_tag="VN444_01915"
     CDS             complement(1893..>2804)
                     /locus_tag="VN444_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWR20598.1"
                     /translation="KKGLQGQYPPGSIFKLVTALAALEKGAITPETKFSCDGSFSLGS
                     HVFHDWKKGGHGTLNLQQGIANSCNIYFYNTALKTGIEEITRVARELGLGATSGLGLG
                     DEARGVIPSPGSTSTKPGGWYPGNTLMAGIGQGMVTVTPMQAVMMVSAIANGGTLYRP
                     WVVRRVETLSGELIEEYGPEFVRKVNIDSDNLAIVREGMQAVVSEGTGGRAKIPGLRV
                     AGKTGTAQVVGNGGPLRGDQRDHAWFVAFAPADDPQIAIAVVVEHGGFGGQVAAPIAK
                     SLLETWFTRPKESQTVQAAEPEPAEGD"
BASE COUNT          601 a          839 c          841 g          523 t
ORIGIN      
        1 cggggtaagc ggaaacttga cgtagatgga cggcgaggta tgatcggcat actcgacctc
       61 ggcctcagct aaggcggtcc ggcatgaggc gcaccagtga accggcttct tgcctttgta
      121 cactgctccc gttccgaata acctccccag ctcacggacg atcgccgcct catacatgta
      181 atccatggtg agatacgggg ccgaccagtt gccgagcacc ccgaggcgtc tgaactcttc
      241 ccgctgaata tcgacgaacc cggcggcgta ctcacgacag agctgtcgct tttcgaccag
      301 cgacacctcc gtcttcttgc tgccaagatt cttatcgacc tggtgttcga tcggaagccc
      361 gtggcaatcc catcccggga cgtaggtcgc gttatagccg aacatcgact tcgacttgac
      421 cacgaagtcc ttcaggatct tattgagcgc atgaccgata tgaatatggc cgttcgcata
      481 gggaggaccg tcgtggagga tccagacctg tcgatcagcc cggacctcgc ggagcctatc
      541 gtacagaccc gaagcgtccc accgcttcag gatggacggc tcgagcaccg gcagatccgc
      601 cttcatcgga aaggcggtgt tgggcaggtt cagtgtctct ttatagttca tagcaatcag
      661 ccgtcagcat tcagctatca gcctctgaca cagcaggggg attccataaa aaccggccgc
      721 agaacgctta ctttcataac agtgacaacg gcgtctgtca aggcctcatg cccgctcaat
      781 acagatagcg atgcttctgg atattgagca ggagaccgac tgccagcatg ttcactagca
      841 tggcagagcc gccgtaggag agtagtggga gagggacgcc gaccaccggt ataatcccga
      901 ccaccatccc gacgttgatc acaacctgca gcgccatcat gctgacgata ccgaagctcg
      961 tgaccatggt gaacaggtcg cgggcatctt tggcgaggcg caggcccttt gaaaagagat
     1021 aggcgtagag caggagcagg ccgagggagc caatgaagcc ccattgctcc gcaaagccgg
     1081 cgaagatgaa gtcggtgtga ttcgccggca gaaagttaag ctggctctgc gtggccgcca
     1141 tccatccctt tccgaggagt cctccagagc cgacggcgat cttcgattgg gcgaggtgat
     1201 agcccgcgcc catgggatcc atgccaggat caataaacac gaggagccgg ctcttttggt
     1261 agtcatgtag gaagcgccag agcacagggg tgatgaccgc tcccaacgta ccgagcataa
     1321 cgaagtagcg tatcttcagc cccatcagca ccagaatgct ggcggaggtg aagagcagca
     1381 ggatggcggt gccaagatcg ggttgccgca ggacgaaagc cgcaggcaag agggtcagga
     1441 ggatcggcgg gagaaagatc cgaggtgctc gtagatcgtc cttgtgatct tcaaagtagc
     1501 gggccagcag gaggatgagt gacagcttca tgaactctga cggttgaaat gcgaacgatc
     1561 cgatgctgag ccatcgctgg gcgcccatgc cggctcggcc gatcagggcg gtgagcaaca
     1621 gggtcgcaag aatcacgcca tagagaggat aggcaaggcg ccagatcgtc cggtagtgga
     1681 gcccgcagag caggatcatg gcgaagagcc ccatcaccgc ccaggtcgcc tgcttgaggt
     1741 acagcgatct ccggaagagg gaggaatgca ggccgctcgt gctgtagatg atcagtacgc
     1801 tcagggcggc aagcccggca gcggaggcag ccaatcgcca gtcgaaggcg ggcagggcgc
     1861 gacggtcgat caacatgaga caggcgcttt catcagtccc cttcggccgg ttccggctca
     1921 gcagcctgaa ctgtctgtga ctccttgggg cgcgtgaacc acgtctccag gaggctcttc
     1981 gcaatcggtg cagctacttg gccaccgaac ccgccatgct ccactactac ggcaatagcg
     2041 atctgaggat cgtctgctgg cgcgaaggcg acgaaccagg catggtcccg ttgatcccct
     2101 ctgagcgggc caccattccc caccacctgg gccgtccccg tctttccggc caccctgagc
     2161 ccggggatct tggcgcggcc gcctgttccc tcgctcacaa ccgcctgcat cccctcccga
     2221 acgatggcga ggttatcgga gtcgatgttc accttccgga cgaactccgg gccgtactct
     2281 tcaatcagtt ccccgctcag cgtttctact cttcgcacga cccatgggcg gtagagggtc
     2341 ccaccgtttg cgatggcgga gaccatcatc accgcctgca tcggcgtgac ggtaaccatc
     2401 ccttgaccga tcccggccat caaggtgttg ccgggatacc atccaccagg ctttgtcgag
     2461 gtgctcccag gggatgggat gacccccctc gcttcatcgc ccagcccaag cccagaggtg
     2521 gcgccaagcc ccagctcacg ggcaacgcgg gtgatctcct cgatcccggt tttgagggca
     2581 gtgttgtaga agtagatgtt gcaagagttg gcgatcccct gctgaagatt cagcgtcccg
     2641 tgaccgccct tcttccagtc atggaagacg tgagagccga ggctgaagga gccatcacag
     2701 gaaaacttcg tctcgggagt gatggcgcct ttctcgaggg cggcaagcgc ggttaccaac
     2761 ttgaagatag agccgggtgg gtactggccc tggagcccct tctt
//
LOCUS       DATMXI010000103         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_164530, whole genome shotgun sequence.
ACCESSION   DATMXI010000103 DATMXI010000000
VERSION     DATMXI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164530"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..825
                     /locus_tag="VN444_01920"
     CDS             67..825
                     /locus_tag="VN444_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWR20599.1"
                     /translation="MASLPRVHKERLDRVLAAQGLVPSREAATRAVLAGEVSVDGVVV
                     DKPAKLVPLDARIQVVQPALYVSRSGDKLAAALDAFQIDPKGTIGLDVGCSTGGFTDC
                     LLQRGATRIYAVDVGYGQFEWRLRQDSRVVLLERTNIRYVDRTEVPEPIDLAVIDVSF
                     ISLTLVLPAVVHLLNSAAVIVALVKPQFEVGKGQVGRGGIVRDDVQREAVTEKVIHCA
                     TQLGFQLTGVLDSPVIGRKGNREILVGFARTITA"
     gene            880..1803
                     /locus_tag="VN444_01925"
     CDS             880..1803
                     /locus_tag="VN444_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWR20600.1"
                     /translation="MKVLVTGGAGFIGSHLVDRLIQEGHEVVVVDNLSTGKRRNLNRA
                     ARFVKLDIQSSGLERVFRNERPHMVMHLAAQMDVRKSVEDPMFDAQVNVLGTLNVLQQ
                     SIKHGVRKVVFSSSGGAIYGEQEIYPAPESHVTRPLSPYGVSKLCGEQYLSYYQRVNG
                     LQIVSLRYANVYGPRQDPEGEAGVVAIFIQKLLNNEQAIINGNGRQTRDFVYVEDVVE
                     ANLAAMGQDIQGTYNVGTGEETSVNDLLGILVRLTNSTCKEVHGPAKGGEQARSVIDS
                     GKLRHELSWESRTELSEGLKRTVDYFRERMG"
     gene            complement(1864..2184)
                     /locus_tag="VN444_01930"
     CDS             complement(1864..2184)
                     /locus_tag="VN444_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR20601.1"
                     /translation="MGFVISPKELKTRLDKGDKLVLLDVREDWEYSLAKLDGSTLIPL
                     GTLPQSLSKLSHDSEIIAICHHGMRSADATNFLLQQGFPNVKNLVGGIDAWSTQVDGT
                     VPRY"
BASE COUNT          560 a          561 c          768 g          647 t
ORIGIN      
        1 tcctagcgtt ccttgcatag gtttctgctc ctgttcaggc gctcatgcgc ggtctccctg
       61 gtcgtgatgg cttctctccc gcgtgttcac aaagaacggt tggaccgtgt gcttgcagcc
      121 cagggtttgg tgccgagtcg ggaagctgca acgagagctg tcttggcagg agaagtatcc
      181 gtcgacgggg tcgtggtgga taagcctgcg aagttggtgc cgttggatgc gcggatccag
      241 gttgtgcagc ctgccttgta cgtcagtcga tctggtgaca aactcgccgc ggcccttgat
      301 gcctttcaga tcgacccgaa ggggacaatc ggtttggacg taggctgttc gacgggtgga
      361 tttaccgatt gtttgttgca gcgaggggcc actcgtattt atgctgttga cgtcgggtat
      421 gggcagtttg agtggaggct tcgtcaagat tcacgtgttg tcttgctcga acgaaccaac
      481 attcggtatg ttgaccgtac agaagttcct gagccgatcg atctggcggt gatcgacgtg
      541 tcatttatct ctctgacact ggtcttgccc gcggtcgttc acctactcaa tagcgcagcg
      601 gtgatcgtgg cgttagtgaa gccacaattt gaggtcggta agggccaggt ggggcgaggg
      661 gggattgtgc gcgatgatgt gcagcgtgag gcggtgactg agaaggttat tcactgtgcc
      721 acgcagttag gatttcagct gacaggggtc ctcgactctc ccgtgattgg acggaaaggg
      781 aatcgggaaa ttcttgttgg ttttgcgcgc acgatcacag cctgatcagg cacacgatgg
      841 atagatgatg atgcggagtc ataggcgatg ggggcatgca tgaaagtact tgtgaccgga
      901 ggcgctggtt ttatcggttc gcacttagtc gatcgtctga tccaggaagg gcatgaggtt
      961 gttgtggtgg acaatctgtc cactggaaaa cggcgaaatc tcaatcgcgc tgcccggttc
     1021 gttaagcttg atatccagag ctcgggactt gagcgggtgt ttcgcaacga aagacctcat
     1081 atggtcatgc accttgctgc gcagatggat gtccgaaaat ctgtggaaga tccgatgttt
     1141 gatgcccagg tgaatgtcct tgggacacta aacgtcctgc agcaatcgat caagcatggc
     1201 gtgcggaaag tggtgttctc gtcatcaggt ggggctatct acggcgagca agagatctat
     1261 ccggcccctg agtcccatgt cacgagacca ctgtcgccat acggtgtcag taaactgtgt
     1321 ggggagcagt atctttccta ctatcaacga gtgaacggac ttcaaatcgt aagcctccgt
     1381 tatgccaatg tgtatggtcc acggcaagat cctgagggag aggcaggggt ggtcgcgatt
     1441 tttattcaga agctattgaa caacgagcag gcgatcatca atgggaatgg acggcaaacg
     1501 cgagactttg tgtatgtaga agatgtcgtt gaagccaatc ttgcggcgat ggggcaggat
     1561 atacaaggga cgtataacgt cggaacaggg gaagagacct ccgtcaatga tttgctcgga
     1621 attttagtcc gccttacgaa ttcgacatgc aaggaagttc atgggccggc caaaggtggc
     1681 gagcaggcac ggagtgtcat tgattcaggg aagctccggc acgaattatc ctgggagtca
     1741 agaactgaac ttagtgaagg gctcaagcgc accgtcgatt atttccgcga acgcatgggc
     1801 taggtctgac gccttggtca tggcggggca cgcaggcgta ccacgcgcgg tgatcaggtt
     1861 gggtcaatat cgaggaacgg ttccgtctac ctgggttgac caggcgtcaa ttcctcccac
     1921 caaattcttc acatttggga agccctgttg aagcaggaaa ttcgtggcat cggcgcttct
     1981 catgccatgg tggcagatgg cgatgatttc tgagtcgtgg ctcaatttag atagcgattg
     2041 agggagcgtt ccaagaggga tgagcgttga gccatccagt tttgcgaggg aatattccca
     2101 gtcctctctc acatcaagta ggaccaattt gtcgccttta tcaaggcgtg tcttcagctc
     2161 tttcggggaa atgacaaagc ccatagggtg ccctccttac ttgtgtcgtt aataatagtg
     2221 agcggagaaa agcttgtcaa tgagaccgat ggtgcctccg gtctgtattg ttcgtataga
     2281 cattagtcaa ctttttccga gccttgagtt ttcttcttca tggagagggt agtcggacat
     2341 actgcacatt tttgtcaggt atgtctgcat attgattcat agcaggtcag ttttaatatg
     2401 acgccagctg cccaaggtga ggctgttagt tagcttatgt ataagatatt gattggttgt
     2461 gacaatcata tgcgcacata cgtggattgg gcccagaaat tgctttactt caagagagaa
     2521 cgtggcaggc caggaa
//
LOCUS       DATMXI010000104         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_233507, whole genome shotgun sequence.
ACCESSION   DATMXI010000104 DATMXI010000000
VERSION     DATMXI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233507"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN444_01935"
     CDS             <1..214
                     /locus_tag="VN444_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWR20602.1"
                     /translation="KRWLMGTHQGAIRAQHLDYYLDEFTFRFNRRTSRSRGKLFYRLV
                     QQAAAVEPVPYTAMLKHTRGRKPRHH"
     gene            426..>2475
                     /locus_tag="VN444_01940"
     CDS             426..>2475
                     /locus_tag="VN444_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR20603.1"
                     /translation="MGSLLWTILTPQGFEPHGYCFLWTRPLLWLYIVSDSLITLSYYS
                     IPIALIYFVRKRRDLAFKRVFLFFAVFIIACGTTHLMALWTIWSPLYWLDAVVKAVTA
                     TVSVVTAVALWPLIPKALALPSPAQLEAANLALQEEVRERHRAQEALHAAYDEMEGRV
                     CERTRELTSTTEVLQAEIAEHELAEQALRESEAKYWALVTQINDGLFVVDVQGVITFG
                     NQALARIHGVDSPEQLVGRSLFEFIAPIALDHVKELFTGAVETGITADVVETQIVRSD
                     GETAFVEVKSVPILKDDRIAGFRGVLRDVTERKRSELALRETYDLLKAVIDASAAGIT
                     ILDREGNVKLWSPAAERMFGWQKEEVLERPLPTVRSDRREEHRTLLERALTGDTFTGV
                     EVVRQKKDGSPIEISLWTAPLRDEKDGVTGVVGIMVDITERKQAEARIRLQAAALEAA
                     ANGIIITDRNGTIVWVNPAFTRLTGYTAEEAGGRTPRLLRSGRHDQAFYRNLWETILS
                     GRVWQGETVNRRRDGSLYTEEQTITPVPDERGDIAHFIAIKQDITERRHLEDQLHQAQ
                     KLEAIGLLAGGVAHDFNNLLNGIIGFAEVALRELPEGSKGASYLSRVPRLGHQAAGLI
                     GQLLAFARKAPLERQPLDLNPLPKESGKLLQRTFPETITIQVEPAFETLVANADPGNV
                     Q"
BASE COUNT          525 a          712 c          765 g          473 t
ORIGIN      
        1 gaagcggtgg ctgatgggga cacaccaggg ggccattcgt gcacagcatc tggactatta
       61 cctcgacgag ttcacgttcc ggttcaaccg ccggacctcg cggtcccgcg ggaagctgtt
      121 ctaccgcctg gtccagcagg cggcggcggt cgagccggtg ccgtacaccg cgatgctcaa
      181 gcatacgcga ggacgaaaac cgagacacca ctagatgtag cgtcaagtgg agtcaagtgc
      241 atacccctat attacagatc atatgtgcat tgcatatgat atctccggtt attatcagat
      301 catataagat acttgcaatg aggttagcgt atgattcacg ttcatcgccc tctcgggtgt
      361 aatctggcct gagagggcat tcgcgtattt gggcggcaag attagaggca ggaggaagga
      421 ggcaaatggg ctcgctctta tggaccatct taaccccaca aggcttcgag ccgcacggct
      481 actgctttct gtggacacgc ccgttgttgt ggctctatat cgtctcggat tcgctgatca
      541 cgctctcgta ttattccatc ccaatcgcgc tcatctactt cgtccgcaag aggcgtgacc
      601 tcgccttcaa gcgggtgttt ctgttctttg cggtgttcat cattgcgtgc gggaccaccc
      661 acctcatggc gttatggacc atatggagtc ccctgtattg gctcgatgcg gtcgtgaagg
      721 ctgtgacggc cactgtatcg gtcgtgaccg cggtagcact gtggccgttg atcccaaagg
      781 cgctggcgct acccagtcca gcccaactgg aagccgccaa tctcgcgctc caggaagagg
      841 tccgcgaacg ccaccgagcg caagaggcgt tgcacgcggc gtacgacgag atggaggggc
      901 gagtttgcga acggaccagg gaactgacaa gtaccactga agtattacag gctgaaatcg
      961 ccgaacacga gctggcggag caagctctgc gggaaagcga agcgaaatat tgggcgcttg
     1021 tgacccagat caacgatggg ctgtttgtcg tggatgtcca gggcgtcatc acctttggaa
     1081 accaggcact agccaggatc catggcgttg acagtccgga gcaactcgta ggccgcagcc
     1141 tcttcgagtt catcgcccct atcgcgctgg accacgtgaa ggagctgttt accggtgcgg
     1201 tagagacagg gatcacggcc gatgtcgtag agactcagat cgtacgctct gatggagaga
     1261 ccgcattcgt tgaggtgaag tcggtgccta tcctgaagga tgaccgcatt gcgggcttcc
     1321 gtggggtctt gcgcgatgtc actgagcgga agcggtcgga gctggccctg cgcgagacgt
     1381 acgatcttct gaaagcggtc atcgacgcct cagcagcggg catcaccatt ttggatcgtg
     1441 agggcaacgt taagctgtgg agcccggcgg cggaacggat gttcggctgg caaaaggaag
     1501 aggttctcga acgtccgctg ccgacggttc ggtccgatag acgcgaagag caccggacac
     1561 ttctcgaacg agcactgacg ggcgacactt tcaccggcgt cgaggtcgtt cgtcaaaaaa
     1621 aagacggctc tccgatcgag atcagcctct ggacagcgcc gcttcgagat gagaaagatg
     1681 gcgtcaccgg cgtcgtcggg atcatggtgg atattaccga gcgcaagcag gccgaagcga
     1741 gaatccgcct tcaggcggcg gcgctggagg cggctgccaa cggcatcatc attacggatc
     1801 gtaacgggac gatcgtgtgg gtcaacccgg cctttacgag actgaccggc tataccgccg
     1861 aggaggccgg cggccggacc ccgcgcttgc tcaggtcggg ccgacacgat caggcgttct
     1921 accgcaacct gtgggagact atcctttccg ggagagtatg gcagggtgag accgtcaatc
     1981 gtcgccggga cggcagcctg tacaccgagg agcaaactat tacgcccgtc cccgacgaac
     2041 gcggtgacat cgcccatttc atcgccatca aacaggatat caccgagcgc agacacctcg
     2101 aagaccagct tcaccaggcg cagaagctgg aggcgatcgg cctcctggcc gggggggtcg
     2161 cgcacgactt caacaatctc ctgaacggca tcatcgggtt cgcagaggtc gccttaaggg
     2221 agttgccgga aggctcaaag ggggcctcgt acctcagccg ggtcccgaga ctgggccacc
     2281 aggccgccgg tctgatcggc caactgctgg ccttcgcgcg caaggcccct ctggagcgac
     2341 agccgcttga cctcaacccg ctgcccaagg agagcgggaa gctgctgcag cggacattcc
     2401 ccgagacgat caccatccag gtcgaacccg ctttcgagac ccttgttgcg aatgcagatc
     2461 cgggcaacgt acagc
//
LOCUS       DATMXI010000105         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_233162, whole genome shotgun sequence.
ACCESSION   DATMXI010000105 DATMXI010000000
VERSION     DATMXI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233162"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN444_01945"
     CDS             <1..401
                     /locus_tag="VN444_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20604.1"
                     /translation="IEWQVQEFQSRTGIRTHLTAEPSEIELDREHSTTAFRILQEALT
                     NVAGHAQAAAVSVNLTADAKRLILKVIDNGKGITPQALADRRSFGLLGMRERALLMGG
                     QVTISGRPDQGTTVTLVMPLKRPAITHPVG"
     gene            443..1075
                     /locus_tag="VN444_01950"
     CDS             443..1075
                     /locus_tag="VN444_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR20605.1"
                     /translation="MLRILIVDDHAVVRQGLKQIVGAEPDMRVTGEAANATELLAMAR
                     QASYDVVVLDIHMPGISGLDALKELKHDCPQLPVLVLSMHPEDQVAVRTLKMGASGYL
                     TKESVPEELIKAIRKVVSGGRYVSPTLAERLVADLAIGTDRPPHECLSSREYQVLCMI
                     GSGKSVNNIAAQLNLSVKTISTYRARILAKLQATHTADLIAYAIRNRLVD"
     gene            1366..1779
                     /locus_tag="VN444_01955"
     CDS             1366..1779
                     /locus_tag="VN444_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR20606.1"
                     /translation="MRVFIVDDSAPVLERLAAVVSELAVTIELVGQAQDAPGAAEAIR
                     RLKPDLVILDLRIPGGGGIHVLREIKQHAPAPIVVILTNYPYPQYRKQCHEAGADFFF
                     DKSMDFDKVPALLQQLARGKSNGNHVSDEPRQCSA"
     gene            1931..>2470
                     /locus_tag="VN444_01960"
     CDS             1931..>2470
                     /locus_tag="VN444_01960"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWR20607.1"
                     /translation="MDRRGCLKRARQSGQPARTVAATCLATVAVLGLGLMTRDASAQV
                     QTYFCPSGFEVGVAPAPPIPLTSLKTIPNPVLPNGPNGAPRDDLAVYIANQTAAIRLG
                     KALFWDMQAGSDNKTACATCHAQAGADVRNKNQLNPGANGVFDSFVPNDVLFAGDFPF
                     STPATDDTDNIAGKEYVIRD"
BASE COUNT          543 a          744 c          731 g          452 t
ORIGIN      
        1 cgattgaatg gcaggtacag gagtttcaat cgcgcacggg tatccggaca cacctgaccg
       61 ctgagccctc cgaaattgaa ctggatcgcg agcactccac gaccgctttc cgcatcctgc
      121 aggaagccct gaccaatgtg gccggccatg cgcaggccgc cgctgtctcc gtcaacctga
      181 cagcggacgc gaaacggctc atcttgaagg tcatcgacaa cggcaagggc atcactccgc
      241 aggcgcttgc cgaccggcgc tccttcgggc tgctgggtat gcgggagcgc gcgctgctga
      301 tgggcggtca ggtcaccatc tctggccgac cggaccaggg aaccacggtc acgctggtga
      361 tgccgctgaa acggccggcc atcacccacc ccgtgggtta acaacgcgca acccgcaata
      421 ataataagag gacggacagg ccatgctacg cattctgatc gttgacgatc atgccgtggt
      481 acggcagggg ctcaaacaga tcgtgggcgc agaacccgac atgcgggtga cgggcgaagc
      541 ggccaatgcg accgagctgc tcgcgatggc ccgacaagcg tcctacgacg tcgtcgtgct
      601 ggatatccac atgccgggga tcagcgggct tgacgcgctc aaagagttaa aacatgactg
      661 tccccaactg cctgtgctgg tgctgagcat gcacccggaa gatcaggtgg cggtccgaac
      721 gctgaagatg ggcgcgtcgg ggtatctgac gaaggagagc gtgcccgagg agctgatcaa
      781 ggcgatccgc aaggtcgtga gcggcgggcg gtatgtcagt ccgacgctgg ccgagcgatt
      841 ggtcgcggat ctcgcgatcg ggaccgaccg gcccccgcac gagtgcctgt ccagccgaga
      901 gtatcaggtc ctgtgtatga tcggctccgg caaaagcgtc aacaacatcg ccgcccagtt
      961 gaatctcagc gtgaagacca tcagcaccta tcgggcacgg atcctggcga agctgcaggc
     1021 gacacacacg gcggatctca tcgcctacgc catccgcaac cggctggtgg actgacaaac
     1081 cagggtgtag ggtggagggg ttagggtata gggtttcaaa gaaaacctct ggctcgcaac
     1141 cccgcactcc atctcgatac ctttcaccct acaccctgtc ttttccaccc gctacctaga
     1201 ccctgtcttt tatgccctat accctatctt gtaggacata ccccgacagc ttcatctcca
     1261 aacgtctgac acaaagaaca gacgcctgcc gatacctctt cccttcgtga gacgctaacg
     1321 tttctgtgag taacgttggt agtcgaattc acaggaggag ccgccatgcg agtgttcatc
     1381 gtagatgatt cggcccctgt gctcgaacgc cttgccgcgg tggtctcgga gctggcagtt
     1441 accatcgagc tggtcgggca ggcgcaagat gccccgggag ccgctgaggc aatccggcga
     1501 ctgaagcctg atctggtgat tctggacctt cggattccgg gaggcggggg gatccacgta
     1561 ctgcgagaga tcaagcaaca cgcgccggcc cccatcgtgg tgatcctcac caattacccc
     1621 tacccccaat atcggaagca atgtcacgag gccggggccg atttcttctt cgacaaatcg
     1681 atggactttg acaaggttcc cgcgctgctg cagcagttag ctcgaggaaa gagtaatggc
     1741 aaccacgtgt cagacgaacc ccgacaatgc agcgcataaa agtcggacac aacaatcagc
     1801 gtttgaccga tttccctgca gtcaatgaaa gaccatgctt tgagtaaagg ctgacgtgaa
     1861 ttgaccatac ctcttgctag gctttgaccc ccgaaaggag ggtcagagcc acgttaacaa
     1921 ggagtgcgtc atggacagac gaggatgttt gaagcgagcg aggcaatccg gtcaaccagc
     1981 cagaaccgtt gcggccacgt gtctggcgac agtcgctgtg ctcggtctgg ggctgatgac
     2041 acgggacgca agcgcacagg tacagacata tttctgccca agcggctttg aagtgggagt
     2101 ggcgccggcg ccgcctattc ccctcacgtc cctcaagacg attccgaatc cggtcctccc
     2161 gaacggcccg aacggtgcgc cgagggacga tctggcagtg tatattgcga atcaaaccgc
     2221 cgcgatccgg ttgggtaagg cgctgttctg ggatatgcag gcggggagcg acaataagac
     2281 ggcgtgcgcc acctgccacg cccaggccgg cgccgacgtg cggaacaaaa accagctcaa
     2341 tccgggcgcc aatggggtat tcgactcctt tgtcccgaat gacgtactct ttgccggcga
     2401 ttttccgttc tcgacccccg caacggacga caccgataac atcgccggca aagagtacgt
     2461 cattcgggac
//
LOCUS       DATMXI010000106         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_256904, whole genome shotgun sequence.
ACCESSION   DATMXI010000106 DATMXI010000000
VERSION     DATMXI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256904"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..868
                     /locus_tag="VN444_01965"
     CDS             473..868
                     /locus_tag="VN444_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR20608.1"
                     /translation="MVSHQAFGAYLRECRLKVGYGLRSFAEAIEMQPSNLSNIEHGRM
                     TPPQDPTTLARIADTLGLPEGSKERTRLFDLAVAHKQPALPPDVAAFAARTPGIPVLL
                     RTIENKRLTREELTILTDYINQHLRKPKR"
     gene            865..1509
                     /locus_tag="VN444_01970"
     CDS             865..1509
                     /locus_tag="VN444_01970"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HWR20609.1"
                     /translation="MNPEEVPTYSIQRLEQIAKLLLEERWPGLTIPVDIDYVVEQEPG
                     TILDYLPGLRAVHGVAGAVISHPQEARFTILIDQEVADGNLSFYRFTVAEEFAHLRLH
                     RKLLATVTSLKEVIQLHEWEGYSESDRHAKWLASALLIPAGAVLEDAHRLYPEMVRRV
                     GFANPEAVKDYVVDRLSRKYLVSPSTMRYRLQRWPVDVLKKIDTAMRDELDFLE"
     gene            1669..2025
                     /locus_tag="VN444_01975"
     CDS             1669..2025
                     /locus_tag="VN444_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20610.1"
                     /translation="MVLMGQATTGRVPKVIEAEEFILRDDTGKERAAMRVSADDIVRL
                     EFFGNDGKHCAGLVLLADGSPSLKLRDAMRNRAVLGHTELESRRMDSVQQQPVSSVGI
                     IRQGWSRHVDGAVATK"
     gene            2455..2934
                     /locus_tag="VN444_01980"
     CDS             2455..2934
                     /locus_tag="VN444_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWR20611.1"
                     /translation="MNLQVVSKEWPPGPPMMYGSVPTMSRGYLRICPITVHKRHPEVS
                     PLRVPPVVADDPYAGLTSLDDVIAHLTSTEAGRRAYEQANREWERKAEELIAKGRLSR
                     LRYRRIKAGLTQEALADRVGIKQPNLQRLERPSYRGRLATYQRLAAVLGCDYRELLP"
     gene            2931..3341
                     /locus_tag="VN444_01985"
     CDS             2931..3341
                     /locus_tag="VN444_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20612.1"
                     /translation="MTRSKEAKLDYWLLCDMIRREDNGKLIIVGAYTPDIAVQAVPVM
                     LPMLSFLFKWDLKPGPLRAGSIVLNDPAGSQIFEFPIEEKTSGEAVESAFFAFGISPF
                     GIEKPGTFTLIYRRGNRLSALGSFRVIIQQAEKP"
     gene            3473..3598
                     /locus_tag="VN444_01990"
     CDS             3473..3598
                     /locus_tag="VN444_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20613.1"
                     /translation="MNKRRKTVELFEQMRREYEQYEHGEGTINGVTCKFGVHRRG"
     gene            3876..>4086
                     /locus_tag="VN444_01995"
     CDS             3876..>4086
                     /locus_tag="VN444_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWR20614.1"
                     /translation="MRINEVIPISELRKRQAEVLAALEKGPAILTQHGKGAAVLLSFD
                     AYNRLLEELEDLQDAMDALEAREAPG"
BASE COUNT          944 a         1122 c         1145 g          875 t
ORIGIN      
        1 cccgtaagcc ggtagttcgc gaggatctcg gtccaaatcc acccgcaacc tcattctcac
       61 cacgatgcac ctctctgatt actagagatc atacagttaa cttataccac cttggcctag
      121 gttcgcgtca agcaagaatg acaaccttgc gtctatcgac ttacgtccca catcgcgtcc
      181 caatcgctgc ctaagcgctc acccctccga agttgagcgg aagttgcaga ttcgcgtgaa
      241 atctaccccg ttttgccgcg tcttacacgg ctgagaatta gaggctgtaa tttcttgtcc
      301 tcggcggagc gataacggat tagcgggctc gaccctcaca cgcctccgtc accctctcgc
      361 taactcctgg aaaatggggg ctgtccctgt atttcccctg gcaccccttg acagctctcc
      421 tcctggggca tatagttata ttagatatat aacagccaca cagggggtga cgatggtttc
      481 gcatcaagcc tttggagcat accttcgaga gtgccgccta aaggtcggat atggcctgcg
      541 cagcttcgct gaggcgattg agatgcaacc atctaacctc agcaatatcg aacatggccg
      601 aatgacccct ccccaagacc ctaccacgct ggcgagaatc gccgacaccc tcggcctccc
      661 cgaggggtcc aaggagcgca cacgattatt tgatcttgcg gtcgcgcaca agcagcccgc
      721 cctgccgcca gatgtcgcgg ccttcgctgc gaggacccca ggcattccgg tgttgcttcg
      781 cacgattgag aataagcggc tgacccgaga ggaactgacc atactcaccg actacatcaa
      841 ccagcacctc aggaagccca aacggtgaac ccggaggagg ttccaaccta ctccattcag
      901 aggctcgaac agatcgccaa gctcctctta gaggaacggt ggcccgggct cacgattccg
      961 gtcgatatcg attacgttgt ggagcaggaa ccaggaacca tacttgacta ccttcctgga
     1021 ttacgagcgg tccacggcgt cgcgggtgcg gtgatcagcc acccccaaga ggctcgcttc
     1081 accattctca tcgatcagga agtcgcagat ggaaatctct ctttctaccg atttaccgtc
     1141 gccgaagaat ttgcccatct taggctccac cggaagttgc tggcaacggt cacgtcgttg
     1201 aaagaggtca ttcagcttca tgaatgggag ggatactccg aaagtgatcg acacgcgaag
     1261 tggctcgcct ccgcattact gattcccgct ggagctgttc ttgaggatgc ccaccgactc
     1321 taccccgaga tggtccggcg cgttggattt gcgaaccccg aggcggtcaa agactacgtg
     1381 gtagaccgtc tcagccggaa gtatctcgta tccccttcaa ccatgcgtta tcgattgcaa
     1441 cgctggccgg ttgacgtact gaagaaaatc gataccgcca tgagggacga actggacttt
     1501 ctggagtaag atgtctcaat gtagggtcaa ccccgtagtt gatggaggag ggagtaatga
     1561 gtccaccggc gatggacacg gagagccttg ggcaacggct gggccgtctg gagcgaaagg
     1621 accgctgatt gaagcgggtg tggcgttacg cttcttggag tcgccgccgt ggtgctgatg
     1681 gggcaggcca ccacaggccg ggtgccgaag gtcattgagg cggaggagtt catccttcgg
     1741 gacgacaccg ggaaagaacg cgccgcgatg cgtgtgtcgg ctgacgacat agtacgcctt
     1801 gagttctttg gcaacgatgg caagcactgc gctgggctag tgctcctggc tgacggctca
     1861 ccgagtctta aactccgcga tgcgatgaga aaccgcgctg tcttaggtca tacggagctt
     1921 gagagcaggc gcatggactc ggtccagcag cagcccgtct cttcagttgg tattattcga
     1981 caaggatgga gccgtcatgt ggatggcgcc gtagccacca agtgagtgaa aatcccagca
     2041 ccatgttcat atcgaatgtg atatgatgca gttgggctat gaggttagag cggaggagcc
     2101 tgcgaaatcg gccttgtatt cacttatgag taaggaccca tcctcagcag cgggaatcgt
     2161 tcgagcactc gaagtactca agatcgatcc caatgtccac attacgggag tcaagttcac
     2221 cgagtcaaag gccgttgaga ttttacgtca acagggtttt agagtacgca ctctgaaagg
     2281 gttcgatttc tcaaggtata gagtattcta cttcgtagat agagcgagga gcacggtggt
     2341 agtcaaagaa gtgattcgaa gagccgctga tacttatgat ccacaggccc cgcacatcca
     2401 acgcctacgg aacaactttt taaaatatca cctctttctg gggagggggc aacgatgaac
     2461 ctacaagtgg tgagcaagga gtggcctcct ggaccaccga tgatgtatgg ctctgtgccg
     2521 acaatgagtc gcggctacct tcggatttgc cccatcactg tacacaaacg acacccggag
     2581 gtgagcccac tgcgcgtgcc tcctgtcgtg gcagacgatc cctatgcggg gctaacgtcg
     2641 ctagatgacg taattgcgca tttgacatcc accgaggcgg gccgacgcgc gtatgaacag
     2701 gctaaccggg aatgggagcg gaaggctgag gaattgatcg ccaaagggcg tttaagccgc
     2761 ctccgctatc gccgcatcaa ggcgggcttg acgcaagaag ccttggcaga tcgggtagga
     2821 attaaacaac ccaacctcca gcgacttgaa cgcccgagct accgaggccg tctcgcaacg
     2881 taccagcggc tcgcggcggt gttgggctgc gattaccggg agcttttgcc atgacccgca
     2941 gtaaagaagc gaagcttgat tactggctgt tgtgcgacat gatccgccgg gaggataatg
     3001 gcaagctgat catcgttggt gcatacaccc cagacattgc cgttcaagcc gtacccgtga
     3061 tgcttccaat gctctcattt cttttcaaat gggacctgaa gccgggccca ctgcgtgcag
     3121 gctctatcgt cttgaatgat ccggccggaa gccagatctt cgaatttccc atagaggaaa
     3181 agacatccgg agaagccgtc gaatcagcct tctttgcctt cgggatctcg cctttcggta
     3241 tcgagaagcc gggcaccttt acgctcatct accgtagggg caacagattg agcgcattgg
     3301 gttcattcag agtcattatt caacaagctg agaagcccta atcctaattt tgtgaaagcc
     3361 ccatgggttt gacaacttga tttggcccac ttcgatcatc tgatttggcc caccctctac
     3421 caccacgccg gcttatcatg gtccgggccg aatggccagg aagggaggcc gggtgaacaa
     3481 gaggagaaag acggtggagc tattcgagca gatgaggcgg gaatatgagc aatatgagca
     3541 cggagaggga acgatcaatg gggtaacgtg taagttcgga gtgcaccggc ggggatagta
     3601 cggcaggcct tagctggcgc agtgccaccc gcgcggaaga ggccggcgcg acagcggccg
     3661 aagctcgggt cggtcccggc cttcatcgat gagatctcct gaaaatggag gctgtccctg
     3721 tatttcctgt atttcgcatc gcctccctcc cccctccatg agtcgcttaa cccccttcga
     3781 attatctttg tggcatcgct tgacactcag aacaccctcc gtctatattg gtagcatcta
     3841 acgaccatta gttgcttcca caaggagagt tggaaatgcg gatcaacgaa gtgattccca
     3901 tctctgaatt gaggaaacgg caagcggagg tcctggcggc actggagaaa ggaccggcca
     3961 ttctcactca gcatggcaaa ggggctgcgg ttttgctgag ttttgacgcc tataatcgcc
     4021 tgctggaaga gctggaagac ttacaggacg cgatggatgc cttagaagcg cgggaggctc
     4081 ctggag
//
LOCUS       DATMXI010000107         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_210880, whole genome shotgun sequence.
ACCESSION   DATMXI010000107 DATMXI010000000
VERSION     DATMXI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210880"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..487)
                     /locus_tag="VN444_02000"
     CDS             complement(230..487)
                     /locus_tag="VN444_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWR20615.1"
                     /translation="MNIAEIVPKENHLLYIKADDGQTGLFDVTPYLESEAFAPLKDRS
                     AFEHIHNGGYFIEWECGADLSADTIQARWMPTTNKNAQQGV"
     gene            complement(484..756)
                     /locus_tag="VN444_02005"
     CDS             complement(484..756)
                     /locus_tag="VN444_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HWR20616.1"
                     /translation="MPTISMFYGILIRMFFYDMDKHHVPHIHAEYQGQVAVYSIHDGS
                     VLAGEFPPKKHKLVVAWIEIHQDDLLADWELAVNGKKPFPIRGLDQ"
     gene            complement(1122..1415)
                     /locus_tag="VN444_02010"
     CDS             complement(1122..1415)
                     /locus_tag="VN444_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR20617.1"
                     /translation="MRPRTFCEGCTSPGGPAAADDHRVPDRAAVRLRQESTRNLLDDW
                     KRLRDLERELVMRALHEAGGTQARAAASLGITPRQFAYKMKKYRIIKEFRIEG"
     gene            complement(1393..2730)
                     /locus_tag="VN444_02015"
     CDS             complement(1393..2730)
                     /locus_tag="VN444_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWR20618.1"
                     /translation="MGGVLLQAPAMVLAQQVPPPEVSSSAQAPESPPPAAPKIDSGDT
                     AWMLTSSALVLLMTAPGLALFYAGLVRRKNALGTIMQSFIALALISIQWILFGYSLAF
                     SSDIGGVIGNLAWVGLQGVGLDPNPDYGATVPHQAFMAYQMMFAVITPALITGAFAER
                     MKFSSFLLFILLWSTFIYDPLAHWVWGVGGWLRKMGALDFAGGTAVHISSGVSALAAA
                     LVIGRRRGHPTHPMPPHNLTMAVTGAALLWFGWFGFNAGSAIAANKLAVSAFVVTHVA
                     AAAGTLAWMFTEWSSRGKPTVLGAASGAVAGLVAITPASGFVGPLSAIIIGAGAGVLC
                     YSACILKARLGYDDSLDVVGVHGVGGTWGALATGLFASKAINPDGPNGLFFGNPSQLG
                     VQAIAVLASMALAFVGTIILLKIVDALMGLRVSDEEEGIGLDLSQHDETAYIL"
BASE COUNT          586 a          797 c          792 g          558 t
ORIGIN      
        1 attgataagc ggtccattcc gcttgtggca cggcgcgtca ggtcgtgggc gaagtacttg
       61 gcgtggtgag gggtgaggct catcggcggg ggcgtgtttc ttttcgggat gggctctcgt
      121 cgatgcgtct gctgtgattc cagatgctca cctcgcggac gtgtgccggg tacgcctaac
      181 gcgccgctaa ccggctgccg aagacacgca gcgtgctcgg cagtccgagc taaacgcctt
      241 gttgggcatt cttatttgtc gtaggcatcc accgcgcttg aatggtatct gcagaaaggt
      301 ctgctccaca ctcccattcg ataaagtacc ctccgttgtg aatatgttcg aacgcacttc
      361 ggtctttcag gggtgcgaag gcttccgatt cgaggtaggg cgttacgtca aatagaccgg
      421 tttgcccatc atcagccttg atgtacagca ggtgattctc tttcggaaca atttcggcaa
      481 tgttcattga tcaagtcctc gaatgggaaa aggtttctta ccattaaccg ccagttccca
      541 atcggcgagc agatcgtcct gatgtatttc aatccatgcg acgaccaatt tgtgcttctt
      601 aggtggaaac tcgccagcca acacagaacc atcgtgaatc gagtacacag caacttgccc
      661 ctgatattct gcatgaatat gcggcacgtg gtgcttatcc atatcataaa aaaacatccg
      721 gataaggata ccgtaaaaca tcgaaattgt gggcataggt acggtgctct ccttttacgc
      781 ccaacggcaa ggatcacacg caagcaaggg cgcgtcgcgc gctatttctt gtcgcgctcg
      841 tgaccttcag acccgtgtcg acaaagtccg gatctgcctc acggatcacc tcccattatt
      901 acaataccaa tggggccgtg gtctctcaaa ggcagaacta ttaagtgagc tgcggtaggt
      961 tggggttatt ccggcggcct atctcagcgc tggccccggt aagcccttgt aacgtagaag
     1021 tgcccgcatt gtcgctctgt tcgcagaacc tgtcaaggcc ctgttttgat tgggataggc
     1081 tgccggctgg gatcgagaca ccacgattca agacgtgtgg gtcagccttc gattcgaaac
     1141 tctttgatga tccggtactt cttcatcttg taggcgaact gtcgtggcgt gatgcccagg
     1201 ctggcggcag cccttgcctg ggtgccgccc gcctcgtgta aggcccgcat gaccaactcc
     1261 cgttcgagat cacgaagtct cttccaatcg tccagaaggt ttcgtgtcga ctcctgccgg
     1321 agcctgacgg ctgcacgatc agggacgcga tggtcgtcag cagccgcagg tcctcccgga
     1381 gatgtgcatc cctcacagaa tgtacgcggt ctcatcgtgc tggctcaggt cgaggccgat
     1441 tccctcctcc tcatcgctga ctctcaggcc catcagcgca tcaacgatct tgagcagaat
     1501 aatcgtgccg acgaatgcca gtgccatgct cgcgagcacc gcaatcgcct gcacccccaa
     1561 ctggctggga ttgccaaaga acagcccatt ggggccatcg ggattgatcg ccttcgaggc
     1621 gaacagaccg gttgccaggg ctccccaggt cccgccaacg ccgtgaacgc ccacgacgtc
     1681 aagcgaatcg tcgtagccga gccgcgcctt gaggatacag gcagagtagc agaggacccc
     1741 tgcgccggcc ccgatgatga tggctgataa cggaccgacg aatccggatg caggagtgat
     1801 ggccacgagc ccggccacag cgccgctggc cgcccccaga acggttggtt tgcccctgga
     1861 gctccactcc gtaaacatcc aggccagggt ccctgcggcc gccgcgacgt gcgttacgac
     1921 gaaggcgctt acagccagct tgttggcggc gatagcgctc cctgcgttaa agccgaacca
     1981 gccgaaccag aggagggcag cgcccgtgac cgccatggtg aggttgtgtg gtggcatcgg
     2041 gtgcgtagga tggccgcgac gtcggccaat gacgagggca gcggccaggg ccgaaacacc
     2101 ggaactgatg tggacggccg ttccgccggc aaaatcaagc gcccccatct tgcgtagcca
     2161 tccaccaacg ccccacaccc agtgcgcgag tggatcatag atgaacgtcg accagagaag
     2221 gataaagagc aggaacgaac tgaacttcat ccgttctgcg aaggcaccgg tgataagcgc
     2281 aggggtgatc acagcaaaca tcatttgata tgccataaat gcctggtgcg ggacggtagc
     2341 cccatagtcg gggttggggt ccagccctac cccttgaagc cctacccagg cgaggttccc
     2401 gataacgccc ccaatatccg agctgaatgc cagagagtac ccaaacagta tccattgaat
     2461 gctgatcagg gccaaggcga tgaagctttg catgatcgtt cccagcgcat tctttcgacg
     2521 gacaagcccg gcgtaaaaca gcgcaagccc aggcgccgtc atcagcagga ccaatgccga
     2581 ggaggtgagc atccatgccg tatctcccga gtcgatcttc ggcgctgctg gcgggggcga
     2641 ttcaggggct tgggctgaag aagagacttc gggaggagga acctgttggg cgaggaccat
     2701 tgctggggct tgcagcagta cgccccccag aac
//
LOCUS       DATMXI010000108         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_40157, whole genome shotgun sequence.
ACCESSION   DATMXI010000108 DATMXI010000000
VERSION     DATMXI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40157"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VN444_02020"
     CDS             complement(<1..931)
                     /locus_tag="VN444_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWR20619.1"
                     /translation="MVTPGLTLDTVIAALREIKYPGMSRDIVSIGVVKDARLDGADVY
                     LDLQIPSEDREVIAKVEAAVRETLARVPGIGDMRIQAGPRPASPDSAPGPSPLPGVRR
                     IIAVASGKGGVGKTTVSVNLALALAQSGAAVGLLDADIYGPNVPRMLGEPGRPKADDG
                     KIVPLVRYGLKVISVGYLLGDRSPIIWRGPLVAQALRQLLHDVRWGELDYLIVDLPPG
                     TGDAQLTLVQAVPLTGGVIVTTPSAVALMDAEKGLQMFREARVPILGIVENMSYFICP
                     HCQGETDIFSRGGGRKVSESLGVSFLGEIPINPA"
     gene            complement(925..1266)
                     /locus_tag="VN444_02025"
     CDS             complement(925..1266)
                     /locus_tag="VN444_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWR20620.1"
                     /translation="MTESETSPVTEEQIYNTLRKLIDPELGVNIVDLGLIYNVQIDDG
                     QVGIRMTLTTRGCPLHGTFVQAIERALNELDGVTGVAVEVVWEPAWNPDMITPEGKQA
                     MAGAGRGGPAW"
     gene            complement(1335..2660)
                     /locus_tag="VN444_02030"
     CDS             complement(1335..2660)
                     /locus_tag="VN444_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20621.1"
                     /translation="MAGSKPGSGNSAPARLPSLWVPLRYFVTAQVAFVTAMLWAPWQV
                     SNLLDFYYQGHDLALTHLLTLGWITMTIMGASFQLVPVALETTLWSERLACWQYWIML
                     FGVAMMVSHFWIGHHEGLAFGAGLVLVAVILFLINMGRTLWQLPHWDIVGRHMAAALV
                     YLASTVIMGNLMALDKIFDFLGGQVLSTIHAHAHLAGIGWVTMMIFGASYKLIPMFSL
                     SELRDERPAYWEFWLLHVGLAGLYVTLLLQSPWATLFALLIAAAVGLFLWTMRDVMRV
                     RRRPQLDWGMRHTMSAMTTLAIVTILGLWLSTGWVPSEEFAARLAFGYGVLALLGWVS
                     AMIIGLMYKIIPFLVWHHRYSDLIGLRSVPAAAQILGESTPRIEFWLLYAGIAMTAAG
                     VVFTSWLLLQAGTLVLALAGLTFAVAVCRIYCHLVPRLTPLPEAQIVGT"
     gene            complement(2629..3240)
                     /locus_tag="VN444_02035"
     CDS             complement(2629..3240)
                     /locus_tag="VN444_02035"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HWR20622.1"
                     /translation="MTNESSCHEGHAKPTPEPKSCDTPTGTPMPDVLRDLPDDRVVIL
                     DIREHVRGGEEPFQRIMQTVMSLRDDQVLKLCNIFEPVPLYAALAQRGFAHWTQRRGP
                     EDWCIMFYRAGTEAATSPASCTSSRPPEPVGDAIVVDARGLEPPQPMAKILENLPQIA
                     AGGKILAITDRRPMLLYPKLEERGFVFSTEETTHGWFETRIWK"
     gene            complement(3360..>3793)
                     /locus_tag="VN444_02040"
     CDS             complement(3360..>3793)
                     /locus_tag="VN444_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWR20623.1"
                     /translation="FNEVPIFDGGPNPANAQAVEPAALWGIRREGMRRLVEEHPAIAI
                     GFLKAFAGKLRYFTRKVEDLSFRSVTSRVAKFLLEMAEDDGRGGLRLKQQFTQQEIAS
                     VVGTAREMIGRAFKALEKEGAIKLDRHQVIIVSRAAMIRML"
BASE COUNT          774 a         1221 c         1112 g          686 t
ORIGIN      
        1 tagccgggtt gatggggatc tcccccagga aagacactcc aagcgattca ctcaccttgc
       61 gtccaccgcc ccggctgaag atgtcggttt ctccctggca gtggggacag ataaagtagc
      121 tcatattctc cacaataccc aggatcggga cgcgggcctc acgaaacatc tgcagacctt
      181 tctctgcgtc catcaaggcc acggcggagg gggtagtcac aatgacccca ccggtgagcg
      241 gcacggcctg cacaagggtc aactgggcat cccccgtccc tggggggaga tctacgatca
      301 ggtagtccag ctcgccccaa cgtacatcat gcaggagctg gcgcaaggcc tgggctacca
      361 agggcccacg ccagattatc ggcgaccgat cccccagcag ataccccacc gatattactt
      421 tcagaccgta ccgcacaagc gggacaatct ttccatcatc tgccttggga cggcccggct
      481 cgcccagcat ccgtggaaca ttcggtccgt aaatgtcagc atccagaaga ccgaccgcag
      541 ccccagactg cgccagagcc aaggccaggt tgacggatac agtagtcttg cccacaccgc
      601 ctttaccgga agcgacggcg atgatacgcc gaacgccagg gagtggagac ggtccgggcg
      661 cggaatccgg ggatgcgggg cgtggaccgg cctggatccg catatcaccg atcccaggca
      721 cgcgggcgag cgtctcgcgg accgcagcct ccaccttggc aatcacctca cggtcctccg
      781 acgggatctg gagatcgagg tacacgtcag cgccatccag ccgcgcatcc tttaccacac
      841 caatggacac gatgtcgcga ctcatccccg ggtactttat ctcgcgaagc gccgcgatca
      901 ccgtatcgag cgtcaaccct ggcgtcacca tgcggggcct ccccgtccag cgccggccat
      961 agcttgcttc ccctcagggg tgatcatgtc cggattccag gcgggctccc acacgacctc
     1021 gacggccacg ccggtcacgc cgtccagttc gttgagggcg cgctcgatcg cctggacgaa
     1081 ggtgccatgc agggggcagc cgcgcgtggt cagggtcata cggatgccga cctggccatc
     1141 atcaatctgc acgttgtaga tgaggcccag atctacgatg ttgactccca actccggatc
     1201 aatgagcttt cggagcgtgt tgtagatctg ctcttcagtc actggtgatg tttctgattc
     1261 cgtcatcgtg cctccttttc atgtaccttt agtaccttca cttcttgaac cttcccgcgg
     1321 atcaaccgcg ggtcttatgt tcccacaatc tgcgcctccg gaagcggcgt caggcgtgga
     1381 accagatggc agtagatccg acagacggcg acagcgaacg tcagacccgc cagcgccaat
     1441 acgagcgtac ctgcctgcag gagcagccag gacgtaaaga ccactccggc cgcggtcatc
     1501 gcgatgcccg cataaagcag ccagaactct atccgaggcg ttgactcacc aagaatctgg
     1561 gcggcggctg gaacagaccg cagtcctatg agatcgctgt aacggtgatg ccacaccaga
     1621 aacgggatga tcttatacat caggccgatg atcatggcgg agacccagcc caggagtgcc
     1681 aggaccccat agccaaaggc cagccgggcg gcaaactcct cgctcgggac ccaaccggta
     1741 ctcagccaca accccaggat cgtcacgatc gccagcgtgg tcatggcgct catggtatgc
     1801 cgcatccccc agtccaattg cggacgccgg cgcacccgca tgacatcgcg cattgtccag
     1861 aggaacaggc ccaccgctgc ggcgatcagg agggcgaaca gcgtggccca cggactctgc
     1921 agtaggagcg tcacatagag gcccgcgaga ccgacgtgga gcagccagaa ctcccagtag
     1981 gccggccgct catcgcgaag ctcgctgagg ctgaacatgg ggatcagctt gtagctcgcc
     2041 ccgaagatca tcatagtgac ccagccgatg ccggccaggt gggcgtgggc gtggatcgta
     2101 ctcaagacct gacctcctaa gaagtcgaaa atcttatcga gcgccatcag gtttcccatg
     2161 atcacggtgg aggccaggta gacgagcgcg gccgccatgt gtcgccccac gatgtcccag
     2221 tgggggagct gccacagcgt gcgccccata ttgatcagaa agaggattac ggcaaccagt
     2281 accaggccgg cgccgaaggc caacccctcg tgatgaccga tccagaaatg gctcaccatc
     2341 atcgcgacac cgaacagcat gatccagtac tgccagcagg ccaggcgctc gctccacagc
     2401 gtggtctcca gggcgaccgg gaccaactgg aacgacgccc ccatgatcgt catcgtgatc
     2461 cacccaagag tcagcagatg ggtcagggca aggtcatgcc cctggtagta gaagtccagc
     2521 agattgctga cctgccatgg cgcccagagc atggcggtca cgaaggctac ctgggccgtc
     2581 acaaaatagc gtagcggcac ccagagggac ggcagcctgg ccggggcgct atttccagat
     2641 ccgggtttcg aaccagccat gcgttgtttc ctcggtggag aagacgaagc cccgctcctc
     2701 aagcttcggg tagagcagca tcggccgccg gtcggttatg gccaggatct ttccacccgc
     2761 tgcgatctga gggagattct ccaggatctt cgccatcggc tgcggtggct cgagtccacg
     2821 ggcgtcaaca acgatggcgt ccccgacagg ctccggcggc ctcgacgagg tgcaggacgc
     2881 cggagaggtc gcggcctcgg tccccgctcg ataaaacatg atgcaccagt cttcagggcc
     2941 acgccgctgc gtccagtggg cgaaaccccg ttgcgccaag gcagcgtata gcggtaccgg
     3001 ctcgaagata ttacagagct tgagcacctg gtcgtcccgg agggacatga cggtttgcat
     3061 gatcctctga aacggctctt cgcctccccg cacatgttca cgaatatcca ggatcaccac
     3121 gcgatcatcg ggcaggtccc gaagtacgtc gggcattggc gtacccgtcg gggtgtcgca
     3181 gctctttggt tcgggcgttg gcttggcgtg cccttcatgg caactgctct cgttggtcat
     3241 aagtcttttg cctccttttt tctcgggtta cttactcgtc gtcgagtata gcataggggg
     3301 ttgattctgg ggtgtctgcg actttagtct cacgtagtgc gagaagggca acagtgaggt
     3361 cagagcatgc gaatcatggc ggcccggctt acgatgatga cctggtggcg atcgagcttg
     3421 atggctcctt ccttttccag ggccttgaag gcccggccga tcatctctcg ggccgttccc
     3481 acaacggacg ctatctcctg ttgcgtaaac tgctgcttca ggcgcagacc tcctctgccg
     3541 tcatcctcgg ccatctccaa gaggaacttg gccacgcggc ttgtcacgct gcgaaacgac
     3601 aggtcctcca ccttgcgggt aaagtagcga agcttcccgg caaatgcctt gagaaagcca
     3661 atcgcgatgg cagggtgctc ctcgaccagg cgccgcatcc cctcccgccg gatgccccag
     3721 agcgccgccg gctccaccgc ctgggcgttg gcggggttcg gcccgccatc gaagatcggg
     3781 acctcattga aac
//
LOCUS       DATMXI010000109         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_98390, whole genome shotgun sequence.
ACCESSION   DATMXI010000109 DATMXI010000000
VERSION     DATMXI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98390"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VN444_02045"
     CDS             complement(<1..1380)
                     /locus_tag="VN444_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWR20624.1"
                     /translation="MKLIRTLLDRLSDSLSLSVPEMRGLATELSNVPPLRLVSDKSVL
                     SAPIDSATRRQANPISQVDTLDDAIRRSQAWFLSRQHPAEGYWVAELEADTTLTAEYL
                     MLRRFLDRVDPERERRAVYYLRAMQLPDGGWPIFYGGPSEISASVKAYFALKLSGISA
                     DEPFMVRARDRILAMGGVVQANVFTKIALAQFDQYDWEGIPHMPVELMLLPKGFYFNI
                     YAISYWSRCVLTPLLIIFAHRPVCQIPREQGIEELYHIPRKEIRYWKFPPFNKDQKWF
                     TLHNFFVALDAMLKLYDRVPLMWLREKALHRATTWMLEHMKGSGGSGAIYPAMANSIV
                     ALRCLAYQVDDPLVQKALHEIESLEVYDTVSIGDQRVEALHLQPCHSPIWDTALLVNA
                     LIETGMPEDHPSLQKAAAYMVSRQTKTVGDWKISSPDAEPGGWYFQFENEFYPDVDDS
                     AVVLMALSKV"
     gene            complement(1579..>2089)
                     /locus_tag="VN444_02050"
     CDS             complement(1579..>2089)
                     /locus_tag="VN444_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWR20625.1"
                     /translation="RINRTTKDMFASTFAELQQKFSEIFVKFFPGGRAELQLVEVPAD
                     ETGEGRGPQEPGVDIVVQPPGKRLKSITMLSGGEKTLTAMALLFASFLIRPTPFCILD
                     EIDAPLDEENIGRFTGVLRELSRDAQFMVITHSKQTMAIADSLFGVTMEEPGISKIVS
                     VRLSNLQPV"
BASE COUNT          464 a          615 c          563 g          447 t
ORIGIN      
        1 gactttcgac agcgccatca acaccaccgc cgagtcatcg acgtcgggat agaattcatt
       61 ctcaaactgg aagtaccacc ctcccggctc cgcatcggga gacgagattt tccaatctcc
      121 caccgttttc gtttggcgcg acaccatgta cgccgccgcc ttttgaagcg atgggtggtc
      181 ctccggcatc ccggtctcga tcaaggcgtt cacgagcaac gccgtgtccc aaatcggcga
      241 gtggcagggc tgaagatgca gtgcctcgac gcgctgatca ccgatcgata cggtgtcgta
      301 cacttccagc gattcgattt cgtggagcgc cttctgcacc aatggatcgt cgacctgata
      361 ggcgaggcag cgaagggcca cgattgaatt tgccatggcc ggataaatcg cccccgaccc
      421 gccgctgccc ttcatgtgct ccaacatcca ggtcgtggca cgatgcagcg ctttctcgcg
      481 caaccacatg agaggcaccc ggtcgtagag tttcagcatc gcgtccagcg cgacaaagaa
      541 attatgcagg gtaaaccact tctgatcttt gttgaacggc gggaacttcc aatagcggat
      601 ctccttccgc gggatatgat acaactcctc aatcccctgc tcccgcggga tctgacacac
      661 cggtcggtgc gcgaagatga tcagcagtgg agttaagacg cagcgggacc aataggaaat
      721 ggcatagatg ttaaagtaga acccctttgg gagcagcatc agctcaaccg gcatgtgggg
      781 gatgccttcc cagtcatatt gatcgaactg cgccagcgcg atcttggtaa acacattcgc
      841 ttgaaccact ccacccatgg ccagaattcg gtcacgggcc cgaaccatga acggctcgtc
      901 tgcggaaatg ccgctcaact tcaaggcaaa ataggccttc acagaggcgc tgatctcgga
      961 aggtcctccg tagaaaatcg gccatccgcc atcgggcaat tgcatggcgc ggagatagta
     1021 cacggctcgg cgctctcgtt ctggatcgac ccgatcgaga aaccgccgca gcatgagata
     1081 ctcagctgtc aacgtcgtat cggcttcaag ctccgcaacc cagtacccct ctgctggatg
     1141 ttgcctggat agaaaccagg cttgactgcg ccggatcgcg tcgtcgagcg tatccacttg
     1201 gctgatcggg ttggcctggc gacgtgtcgc agaatcgatg ggtgcagaca gcacggactt
     1261 gtccgacacc agccgcaacg gcggaacgtt ggataactcg gtcgccagcc cgcgcatctc
     1321 cggaacggag agggagaggc tatcggatag acgatcgagg agtgttcgaa tcagcttcat
     1381 tctatcggtc tattgaaaat tgtagatgat gaattgaacc cttcgcgtga ctcgcttggc
     1441 cccacaaagc tcacgcgacg agcggcccgc ccataggtcg ggctctgtgg ggtatataac
     1501 agagccccta ctctgacgtc aagaaacaga ggtacggaag tcctctgaag tatggatgtt
     1561 ttagccgacc tgctcttttc aaactggctg caaattgctg agccttaccg agacaatttt
     1621 tgagatccca ggctcttcca tggtgacccc gaacagcgag tctgcaatgg ccatcgtctg
     1681 cttactgtgg gtgatgacca tgaactgtgc gtcccgggac agttcacgca atacgccggt
     1741 aaaacgtccg atgttctctt catccaacgg ggcgtcgatt tcgtccagaa tacagaacgg
     1801 cgtcggtctg attaagaagc tcgcaaacag caacgccatt gccgtcaagg ttttttcacc
     1861 acccgagagc atcgtgatgc tcttcaatcg tttgcccggt ggttgcacca cgatatcgac
     1921 acccggttcc tgcgggccgc gcccttctcc tgtctcgtcc gccggcacct cgaccagctg
     1981 cagttcggct cgaccacccg ggaaaaactt tacgaatatc tcgctgaact tctgttggag
     2041 ctcggcaaac gtgctggcga acatgtcttt cgtcgtacgg ttgatgcgc
//
LOCUS       DATMXI010000110         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_257932, whole genome shotgun sequence.
ACCESSION   DATMXI010000110 DATMXI010000000
VERSION     DATMXI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257932"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VN444_02055"
     CDS             complement(<1..960)
                     /locus_tag="VN444_02055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWR20626.1"
                     /translation="MNFPIIRPTLPSLSDIVRLVGPSWDSGTVTVGPTVRALEEEVCR
                     QTGARYCVALSSCTAGLMLIPGALGLPGGKEVIVPSFTFAATAQALLWNGLIPVFADC
                     LPGTCTIDPEDVEPNLSPDTAAICGVSIFGLPPDYGALLELSRRKGVPVYFDSAQGLG
                     ATYHGQPLGGFGVCEIFSMSPTKVVTAVEGGLLTTNDQTLAERIRSMRDYGKDPVKGE
                     EMHHLGLSARMSELHAAVGLLSLRRMGELVKARKELISIYRDRLGRLPGCWVQKFPAD
                     RTTSGNYFVLFIAESAKRSRDEVYEALKAVGIQTKRYFYPPVHE"
     gene            complement(1023..1652)
                     /locus_tag="VN444_02060"
     CDS             complement(1023..1652)
                     /locus_tag="VN444_02060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWR20627.1"
                     /translation="MDLTVKRLFDIVGSFLILLLFGPVMVLVAIGIVVTMGRPVLFRQ
                     RPPGLGGETFTVIKFRTMRVIEDLAHQGQLLIDQQCLTPLGRILRHSSLDELPQLFNV
                     LLGTMSLVGPRPLLPRYLPYYTERECQRHSVKPGITGWAQVNGRNAISWDQRLAFDVW
                     YVEHQSLLLDFRILSLTFLRVLQRRDINQPCADYVPLDEERRHSPTPLL"
     gene            complement(1726..>2122)
                     /locus_tag="VN444_02065"
     CDS             complement(1726..>2122)
                     /locus_tag="VN444_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20628.1"
                     /translation="NWIVDGDFGFLDMAETIIHLDFPLSLCLWRTTMRSIKRFLKWDF
                     GTMNVLAAVPVRITQLLRLLSEVYWYPSEAVATPQPEPAAGSSKAVIVLKSPKELELF
                     LSSIELPAQPCHLSAERKITPSAIVSTKN"
BASE COUNT          494 a          596 c          587 g          445 t
ORIGIN      
        1 ctcatgaacc ggcggataga agtatcgctt cgtctgaatc ccaaccgcct tcaatgcctc
       61 gtagacctca tcacgactcc gtttcgcgct ctctgcgata aacaacacaa agtaattccc
      121 gctcgttgtc cgatcggctg gaaacttctg gacccaacag cctgggagtc tgcccaaccg
      181 atctcgatag atcgaaatga gttccttgcg cgccttcacc agttctccca tgcggcgcag
      241 ggagagcagc ccgaccgccg cgtgcagctc gctcattcgg gcggacaggc ccagatgatg
      301 catctcttct cccttcacag gatccttgcc gtaatcccgc atcgacctga ttcgctccgc
      361 gagggtttgg tcattcgtcg tcagcagtcc tccttcgacc gctgtcacga ctttcgtcgg
      421 gctcatcgaa aagatctcac aaacgccgaa tccccccagc ggctgaccgt gataggtcgc
      481 gcccagccct tgcgcgctgt cgaaatagac gggcactcct ttccggcgac ttagctcgag
      541 caaggccccg tagtcggggg gcaagccgaa gattgataca ccgcaaatcg ccgccgtgtc
      601 cggagagagg ttcggttcga cgtcttcggg atcgatggtg caggttcccg gcagacaatc
      661 ggcgaacacc ggaatcagcc cattccacaa caaggcctgg gctgtcgcag cgaaggtaaa
      721 cgacggcacg atgacttctt tcccgcccgg caatcccagg gctcctggga tcaacatcaa
      781 tccggccgta caggacgaca gcgccacgca gtatcgcgcg ccggtttggc ggcacacctc
      841 ctcttcgagg gccctgacgg tgggaccgac tgtgacggta ccggaatccc agctcggacc
      901 gacgagtcgt acgatatcgc tcaatgacgg gagtgtaggt cgaatgatgg gaaaattcat
      961 aaccctcctt tggcttggtg ccgctacaat ggccgatggt gttgcacgtg acgatagaaa
     1021 tgttacagaa gcggagttgg ggaatgtctg cgctcctcgt caagcgggac ataatctgca
     1081 caaggttgat tgatatcacg acgttggaga actctgagaa aagttagaga caggatgcga
     1141 aaatcaagga gcagtgattg gtgctctacg taccagacgt caaatgcgag tctttgatcc
     1201 caggaaatgg cattccgccc gttaacctga gcccacccgg taatgcctgg tttgacggaa
     1261 tgccgctggc attcgcgctc agtataatag ggaagatacc tgggcaagag aggacgtgga
     1321 ccgaccagac tcatcgtccc cagaaggaca ttaaagagtt gcggtagctc gtcgaggctg
     1381 ctgtgtcgaa gtattctgcc tagcggcgtc aggcactgct ggtcgatcag caattgtcct
     1441 tgatgagcga ggtcctcgat tacacgcatc gttcggaatt tgatgacggt aaatgtctcg
     1501 cctccgaggc ccggaggcct ctgtcggaac agaaccggcc gtcccatggt cacaacaata
     1561 ccgatggcta ccagaaccat cacaggcccg aagagcaaca ggatgaggaa agatccgaca
     1621 atatcgaaga gacgcttcac agtcaggtcc atggctaata ccgattcatg gtaagagatg
     1681 actcgttccc tgacgcacag aacacagctc tctcctgcgt catgtttaat tcttcgtaga
     1741 aactatggcg ctaggagtga tctttctctc cgccgatagg tgacacggtt gtgccggcag
     1801 ctcaatcgat gacaggaaaa gctccagttc cttgggcgat ttaagaacga tcacggcttt
     1861 agaggatccc gctgctggct caggctgtgg cgtagccact gcttcggatg ggtaccagta
     1921 cacctcggat agaaggcgaa ggagttgggt gatccttact ggaactgctg ctaacacgtt
     1981 catcgtcccg aagtcccact tcagaaatcg tttgatgctg cgcatggtgg tccgccagag
     2041 acaaagagag aggggaaaat caagatggat gatggtttcg gccatgtcga ggaatccgaa
     2101 gtcaccgtcc acgatccagt tc
//
LOCUS       DATMXI010000111         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_98336, whole genome shotgun sequence.
ACCESSION   DATMXI010000111 DATMXI010000000
VERSION     DATMXI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98336"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VN444_02070"
     CDS             <1..1201
                     /locus_tag="VN444_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWR20629.1"
                     /translation="YRALDETDEALFAARTIQDHVANDATRYDDYAIFYRTNAQSRVL
                     EEALRQALIPYVIVGGLRFYERKEIKDLLAYLRWVVNPADSISFKRLVNAPARGIGPA
                     TIAKLELLAMQDKSTIWEACQRAVRERLFNVKQQAAMEGLLHLIEEVRAKSAFTQIPD
                     LIAELITASGYAEELQRDGTPEDQSRLENLKELVTAAQEFVERTAEGGLPAFLDSVAL
                     ISDLDAYTEGRGAVTLMTLHTAKGLEFDTVFMVGLEEGIFPHALAMPDERELEEERRL
                     CYVGMTRAKRRLYLASARQRRLYGNSGFNLPSRFLDEIPPEVLQVQDPWESRGAPPPA
                     FHKHQEEDEPFVDRLHPGARIRHPDFGIGVIRERSGSGDDLKIVVRFNGAGEKKLMVK
                     YAQLEQA"
     gene            1247..1537
                     /gene="gatC"
                     /locus_tag="VN444_02075"
     CDS             1247..1537
                     /gene="gatC"
                     /locus_tag="VN444_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWR20630.1"
                     /translation="MKITLKEVEHVARLARLELTTEEKERMRAQIDSILSYIDKLNEL
                     DTSAVEPTSHVLPMTNVFREDEVMPSLSQEEVLVNAPDRHELFFRVPRILEE"
     gene            1542..>2047
                     /locus_tag="VN444_02080"
     CDS             1542..>2047
                     /locus_tag="VN444_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWR20631.1"
                     /translation="MDPTQLTIHDMQALLATREISAVELVRSVLDRITRLDGQVMAYT
                     TLTEERALEQAGAVDRLLAVGTPLPPLAGIPLAIKDVICTKGVRTTCASKILEPYVPP
                     YDATVIRRLKAQEAVLLGKTNMDEFAMGSSTENSAFFGTRNPWALDYVPGGSSGGSAA
                     AVAAELCAA"
BASE COUNT          437 a          599 c          623 g          388 t
ORIGIN      
        1 ctaccgagcc ctcgatgaaa ccgatgaggc cctcttcgcc gccaggacga tccaggatca
       61 cgtagccaat gacgcaacgc ggtacgacga ctacgcgatc ttctaccgga caaacgcgca
      121 gtctcgcgtc ctggaggagg ccttgcgtca ggccctgatc ccgtacgtca ttgtgggtgg
      181 gctccgcttc tacgaacgga aggagattaa ggatctcctg gcctatttac gctgggtggt
      241 caatcccgcc gatagcatca gtttcaagcg gctcgtgaat gcgccggcgc gcggtatcgg
      301 accggccacg atcgccaaac tcgaactgtt ggccatgcaa gacaagagta cgatatggga
      361 agcgtgccaa cgcgcggtac gggagagact gttcaacgtc aagcaacagg cggccatgga
      421 gggattgctc cacctgattg aagaggtcag ggcaaaatcc gccttcaccc agattccaga
      481 cctgatcgca gaactgatca ccgcttccgg gtacgctgaa gagcttcagc gcgatggaac
      541 gccggaggat cagagccgcc tggaaaacct gaaagagctc gttactgctg cccaagagtt
      601 tgtggagcgc actgcggaag gggggctgcc ggcgtttctc gactccgtcg ccctgattag
      661 tgacctcgac gcgtataccg aaggccgggg cgcggtcacg cttatgacgc tccatacggc
      721 taagggtctg gagtttgaca cggtctttat ggtcgggcta gaggaaggga tctttcctca
      781 cgcccttgcc atgcctgacg agcgtgagtt agaggaagaa cgccgactct gttatgttgg
      841 catgaccagg gcaaagcgac ggctctacct tgcgtcggca cgacagcgac ggctctacgg
      901 caacagtggg tttaacctgc cgtcacgatt cctggatgaa atcccgcccg aagttctcca
      961 ggtccaagat ccgtgggaat ccaggggcgc accgccccct gcctttcata agcatcaaga
     1021 agaggatgag ccctttgtcg accgcctgca tccaggagcc cgcatccggc atcctgactt
     1081 cggcatcggg gtgatccggg aacggagtgg cagcggggat gatttgaaga tcgtcgtgag
     1141 gttcaatggc gccggtgaaa agaagctcat ggtcaagtat gcccaactgg agcaggcgta
     1201 gataaccgct tgccacgcag gacactaatg caagaggaca tgtgccatga agatcacact
     1261 taaagaggtc gagcatgtgg cccggctggc ccgtttggag cttaccacgg aggaaaaaga
     1321 acggatgcgg gctcagatcg actcgatcct gagctatatc gacaagctga atgagttgga
     1381 cacgtctgct gtcgagccga cctctcacgt cctgccgatg accaatgtct tccgtgagga
     1441 tgaggtcatg ccttcgctct ctcaggaaga ggtcctggtc aatgcgccag accggcatga
     1501 gctcttcttc cgggtcccga gaatcctgga ggagtaacgc cgtggacccc actcaactca
     1561 ccatccacga catgcaggcg ctgcttgcga cacgcgagat cagcgctgtc gagcttgtcc
     1621 gctcggtgct ggatcggatc acgcgcctcg atggacaggt catggcgtac acgaccctga
     1681 ccgaggaaag agcccttgag caagccgggg ccgtcgatcg gttgctcgcc gtgggcactc
     1741 ctctcccgcc gttggccggc atcccactgg cgattaaaga tgtcatctgc acgaaagggg
     1801 tgcggaccac ctgcgcatcg aagatcctgg aaccctacgt accaccgtac gatgcaacgg
     1861 tcatccggcg cctcaaagcg caagaggcgg tcctgctggg gaagacgaac atggacgagt
     1921 tcgctatggg ctcctcgacg gagaactcgg ccttctttgg aacccggaat ccctgggcac
     1981 tcgactatgt ccccggcggc tcttctggcg gctctgcggc cgccgtggcg gcggagctgt
     2041 gcgctgc
//
LOCUS       DATMXI010000112         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_127066, whole genome shotgun sequence.
ACCESSION   DATMXI010000112 DATMXI010000000
VERSION     DATMXI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127066"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1819
                     /locus_tag="VN444_02085"
     CDS             335..1819
                     /locus_tag="VN444_02085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWR20632.1"
                     /translation="MAPLQHPAWKSLVVQQGKTLRSAVPTQSVSNGEFAPFGQTADQA
                     RVEQIAAAIIEQSAARQGLTRRDFLKTTGGVAGALLAMNLVFGRFFDIGEIELFESAA
                     FAEQQGDPYFIFDVQTHYVGSHYDPTEAESHRRGGVSKQALLSLRRRIREAGLNPKLA
                     GDTGTLDDLSWRNFVKEVFLDSETNIGLISTPPGPYPQEAVVPPQEMAHIRDEVNRVA
                     GSQRMLAHGLVTPQLGIADLDFMALQAETLKVEAWKCYTGSCPKGFDRGWWMDDERIA
                     YPMLEQARKLNVKRVCVHKGLPLGPVPDYNHPRDLIKASKDFPDIDFLVYHSGLKSTA
                     NMNEGTLRTGEIPWTTEFCRMKQQEPGISNIYMELGSTFGQLVTTQPSACAHLLGQII
                     QTFGADHVLWGTDSIWYGTPQWQIEAFRRFQIPDQLLDRHRYQPLTRRVKEQIFGLNA
                     ARIFGIDVKAKRHEVPKDALGQIRMSYLDEGPEPSHRVYGWVTG"
     gene            1877..>2163
                     /locus_tag="VN444_02090"
     CDS             1877..>2163
                     /locus_tag="VN444_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWR20633.1"
                     /translation="MDKPHKKLDLWNTALELAVDLYRATEGFPKDERYSLTDQIRRAA
                     VSVPSNVAEGAGRQTRKEFINYLHMAQGSLSELDTQLELAKRLGYVEHDNWL"
BASE COUNT          542 a          574 c          603 g          444 t
ORIGIN      
        1 ctttaccggt cttcccatgc tgctcgatca gcagaggggc gtgatgtata gtgtcgggca
       61 agacggcaag gatcaggaag gcgattccct gcgcgatgtc gtcgcaacaa ttcccaagtt
      121 caatcgatcg taaacgagag cgctgctccc tgttcccgcc ccggtccaaa taatccacta
      181 caccctttcg aagccggaga tttctgattt ccccctcttc atggattgat ttctacgtca
      241 aagagccgga tgggctatct ggttaggaga tgcttgacac tgacggtagt atttcacaga
      301 atcgcgcaca ggccagtgca gaggataatc cgccatggcg cctctccaac atcccgcttg
      361 gaaaagcctc gtagtacaac aaggcaaaac ccttcgttct gcggtaccga ctcagagcgt
      421 ctcaaacggg gagtttgcgc ccttcggcca gaccgcagac caagctcgcg ttgaacagat
      481 cgcagcagcg ataattgaac aatccgccgc acgacagggc cttacccgca gagactttct
      541 caaaacgacg ggcggagtgg ccggtgcgtt gctggcgatg aatttggtct tcggacggtt
      601 cttcgacata ggcgaaatcg agttattcga gtctgccgcc tttgcagaac aacagggaga
      661 cccctacttc atctttgacg tacagaccca ttatgtggga tctcattacg accccacgga
      721 ggcggagagt catcgcagag gaggagtgtc caaacaggca ttgctatctc tgcgccgcag
      781 gatccgcgaa gcgggactta accccaagct ggcaggcgat acaggtacac ttgacgacct
      841 ctcctggcgc aactttgtga aagaagtctt tctcgacagt gagacaaata tcggactgat
      901 cagtacgcca ccaggaccct atccgcaaga agccgttgtg ccgccgcagg agatggcgca
      961 tattcgcgat gaggtgaatc gtgttgccgg ttcccagcgc atgttggccc atggactcgt
     1021 cacaccacag cttggcatag cagatctcga cttcatggcc ctacaggcgg aaacgctcaa
     1081 ggtcgaggcc tggaagtgct ataccggatc ctgtccaaag ggattcgatc ggggctggtg
     1141 gatggatgat gagcggattg cctatcccat gttggagcag gcacgcaagc tcaacgtaaa
     1201 gagggtctgt gtccacaagg gattgccgct tggccccgtg ccggactaca accacccaag
     1261 ggatttgatc aaagcctcga aggatttccc cgacatcgac tttttagtct atcactcggg
     1321 gctcaagagc acggcaaata tgaacgaggg cacgctaagg acgggcgaga ttccctggac
     1381 gaccgagttt tgccgcatga aacagcaaga gccaggaatc agcaatatct acatggaact
     1441 cggctcgacc ttcggccaac ttgtgacaac tcagccgtca gcctgcgcgc atctgctggg
     1501 gcagattatt caaacattcg gtgccgatca tgtgctctgg ggaactgact cgatttggta
     1561 cgggacgccg caatggcaaa ttgaggcatt cagacgattt cagattcccg accaactgct
     1621 cgacagacat cgctatcaac ctctcacacg acgggtgaag gagcagatct ttggactcaa
     1681 cgcggccaga atttttggaa tcgatgtgaa ggcgaaacgg catgaagtgc ccaaggatgc
     1741 gctaggccaa atcaggatga gttacctcga tgaaggaccg gagccgagtc accgagtata
     1801 tggatgggta acggggtaga tgccgtgagg cgttaggcgt aaggtgtgag gcgatatctc
     1861 aggaagcggc caaggcatgg acaagccgca taaaaaacta gacctgtgga atacagctct
     1921 ggaactggcg gtcgaccttt atcgtgctac agaaggcttt cccaaggacg aacgctatag
     1981 tctcaccgac caaatccgaa gggctgctgt gagtgttccg agcaatgtcg ccgagggagc
     2041 aggtcgtcaa acccgaaaag agttcatcaa ctacctgcac atggcgcaag ggtcgctgag
     2101 cgaattagat actcaactcg aacttgcaaa aaggcttggg tacgttgagc acgacaattg
     2161 gct
//
LOCUS       DATMXI010000113         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_185534, whole genome shotgun sequence.
ACCESSION   DATMXI010000113 DATMXI010000000
VERSION     DATMXI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185534"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1592)
                     /gene="trmFO"
                     /locus_tag="VN444_02095"
     CDS             complement(216..1592)
                     /gene="trmFO"
                     /locus_tag="VN444_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWR20634.1"
                     /translation="MSVCLPVPTPQTGRTQTEIVVVGGGLAGSEAAWQAAERGAHVRL
                     YEMRPGSVTPAHKTDRLAELVCSNSLKSDSPDDCHGLLKRELTAYGSVIMAAARAHAI
                     PAGSALAVDRDAFATEVTARLARHPRITIVRDEVKAIPEERPVIIATGPLTSERLAGA
                     LRDRFVNYPASADASANAEDLLYFYDAISPIVTGDSIDRTVAFAASRYDKGGDDYLNC
                     PLTREEYRLFWQSIRTAELAPIHPFEEARYFEGCLPIEVLAARGEDALRFGPMKPVGL
                     IDPRTGRRPYAVVQLRLENREGTMYNMVGFQTRLKWGEQHRILRTIPGLKSADFLRYG
                     SIHRNTFINAPTLLLETMQLKDDPGILLAGQLTGVEGYLESAGSGLLAGVNAVRVLRG
                     EAPVVLPPTTAIGSLIRHICHANPRTFQPMNVNFGLLPPLDAPVRAKRERRQALAARA
                     LRDLPDLS"
     gene            complement(1598..4075)
                     /gene="topA"
                     /locus_tag="VN444_02100"
     CDS             complement(1598..4075)
                     /gene="topA"
                     /locus_tag="VN444_02100"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWR20635.1"
                     /translation="MPKSLVVVESPAKAKTIEKYLGKEFVVTASMGHVRDLPAKSLSI
                     DIARDFAPQYEIIEGREKVMSALRKAAKTAEAIYLAADPDREGEAICWHLQQELGAAN
                     KPVHRVTFNEITKRAVREAFNHPSTIDQRLVDAQQARRILDRLVGYQISPLLWDKVKR
                     GLSAGRVQSVALRLIVDREREIKVFVKAEYWSVDVALEGRTPPSLRAGLYKIDGERVN
                     LPDQAQTDQVVRELREQEFRVTDLTRKEKRRNPVPPFTTSKLQQEAVRKLRLTARRAM
                     QIAQQLYEGIEIGEEGAVGLITYMRTDSVRVSSEAQIEAARYIAERFGQPFVPDRPPA
                     YRSGRGAQEAHEAIRPTSVYRDPVSLRQFLTKDQLALYTLIWSRFVASQMPPALYDVT
                     TVTIRGGRFTLRAGGRHQRFAGFMQVYIEGRDEVPETKPTEAKDETAEETAVEEEAEL
                     TLPPLEVGEPLRLLDVTPAQHFTQPPPRFTEATLVKELEERGIGRPSTYAAILSVIQN
                     RDYVVKAQGKFRPTELGGIVVDLLVESFPRVMDYEFTARMETTLDEIEEGQKNWLEEM
                     RRFYEPFSQWVKEAAVGMRNIKAMEEKTEEICERCGSGMVIRWGRFGRFLACTNYPEC
                     KGTRELTAELKGEHGTDEDTDVSAEAVTAPCENCGRPMVMKRGRFGPFLGCSGYPECK
                     TIRKLSQAEAASARPAPQPTDEVCEKCGSPMVLREGRYGRFLSCSTYPTCKHIKPIAI
                     GVKCPQCGSPLSERRTKRGRVFYGCTAYPKCAFAIWDRPIQEPCPQCGAAFLVEKKLK
                     GGGVSIRCSAEGCAYAQDAEAAAQVKA"
BASE COUNT          782 a         1350 c         1195 g          809 t
ORIGIN      
        1 gaagccgctc gctccgcctg aagatacaag agatacgcct caatctgctc caacacctgc
       61 tcacttctcc ttcgttcctg cccggtacga tacgaggaat aggtcgaaag atcaagccca
      121 aacgtgggag ttccaggtgc caggttcaga tctgaattct caggaagcaa tgccgaacaa
      181 cttagaacgt gagaacgtgg aacctgggac tgtcttcagg acagatcagg caggtcgcgg
      241 agggcgcggg cggccagggc ctgccgcctt tcgcgcttgg cccgaactgg cgcatcaagc
      301 ggcggcagca ggccgaagtt cacattcatc ggctggaagg tgcggggatt cgcatggcag
      361 atgtgacgga tcagggaccc gatcgcggtc gtcggaggga gcacaaccgg agcctcgcct
      421 cggaggactc gaactgcatt gacgccggcc aggagtccgc tcccggctga ttccaggtat
      481 ccctccacgc cggtgagttg tccggccagc aagatccccg gatcgtcttt caactgcatc
      541 gtctccaaga gcagcgtcgg cgcgttgata aaggtgttgc gatggatcga cccgtatcgg
      601 aggaagtcgg cgctcttgag gccgggaatc gttcggagga tccggtgctg ctcgccccat
      661 ttcaggcgcg tctgaaaccc caccatgttg tacatcgtcc cttcccggtt ctccagtcgg
      721 agttgcacga cggcgtaagg ccggcgtccg gtcctgggat cgatcaggcc aaccggtttc
      781 atcggcccga accgcaaggc atcctcgccc cgagccgcca acacctcgat cgggagacac
      841 ccttcgaaat atcgcgcctc ctcgaacggg tggatcggcg ccaactccgc cgtcctgatt
      901 gactgccaaa agaggcgata ctcctcacgg gtcagtggac aattcaggta atcgtcccct
      961 cctttatcgt agcgggaggc ggcgaacgcc acggtacgat caatagagtc cccagtgacg
     1021 atcggcgaga tggcgtcata gaaatagagg agatcctctg cgttggcaga cgcatctgct
     1081 gatgccggat agttcacgaa ccgatcacgc agcgcgccgg ccagccgctc ggaggtcaac
     1141 ggaccggtgg caataatcac cggcctctct tccggtatcg ccttgacctc gtcccgaaca
     1201 atagtgatac gcggatgacg cgcaagcctg gcagtcacct cggtggcaaa ggcgtcacga
     1261 tcaacggcta acgccgatcc ggcagggatg gcgtgcgccc gcgcggcagc catgatcacc
     1321 gacccatacg cggtcagctc ccgtttcagc aggccgtggc aatcatccgg cgaatccgac
     1381 ttcaaggagt tgctgcagac cagttcagcc agccggtcgg tcttgtgcgc aggggtgacg
     1441 ctcccaggcc gcatctcata taggcgcacg tgggcgcccc gctccgccgc ctgccaggcg
     1501 gcttcgcttc cggccagacc accgcccacc acaacgatct cggtctgcgt gcgacctgtc
     1561 tgcggtgtcg gcacaggcag gcatacagac aggtcggtca cgcctttacc tgcgctgccg
     1621 cctcggcatc ctgcgcgtag gcgcagcctt cggcactgca acggatcgag accccgccgc
     1681 ccttcaactt cttctccacc aggaaggcgg ccccgcattg cgggcaaggt tcctggatcg
     1741 gacgatccca gatggcaaag gcacatttag gatacgccgt acacccatag aagacccgac
     1801 cccgtttggt gcgtcgctcc gacagagggc tcccgcattg cgggcacttg accccgatcg
     1861 caataggctt gatatgcttg caggtgggat aggtggagca ggacagaaag cggccatatc
     1921 gtccctcccg caacaccatc ggactaccac acttttcgca gacctcgtcg gtcggctgcg
     1981 gcgcaggccg cgcactcgcc gcctccgcct gcgagagctt ccggatcgtc ttgcactcag
     2041 ggtatccgga gcaacccagg aagggcccga atcggccgcg tttcatcacc ataggccgtc
     2101 cgcagttctc gcaaggcgcc gtaacagcct cagcggagac gtcggtgtcc tcatccgtcc
     2161 catgctctcc tttcagttcg gccgtcaact cgcgcgtgcc cttacactcc gggtaattgg
     2221 tacaggccag gaagcggccg aatcgacccc agcggatcac cattccactg ccgcaccgct
     2281 cgcagatctc ctcagtcttt tcctccatcg ccttgatatt ccgcatgccg acagcggcct
     2341 cttttaccca ctgggaaaac ggctcataga agcgacgcat ctcctcaagc cagttcttct
     2401 gtccctcttc gatctcatcc agggtcgtct ccatcctggc cgtaaactcg tagtccatca
     2461 cgcgagggaa gctttccacc agcaggtcaa caacgatccc gcccagctca gtgggtcgga
     2521 atttgccctg cgccttcacc acatagtcgc ggttctgaat gacgctcagg atggccgcgt
     2581 aggtgctggg gcgtccgatc ccccgctcct ccaactcctt caccagtgtc gcctcggtga
     2641 agcgtggcgg aggctgcgta aagtgctgag ccggggtcac atcgagcagc cgcaacggtt
     2701 ccccgacctc gagagggggg agcgttaatt cggcctcttc ctcaacggcc gtctcctcgg
     2761 ccgtttcgtc cttggcctcg gtcggcttgg tctccggcac ctcatccctc ccctcgatat
     2821 agacctgcat gaaaccggcg aaccgctggt gccgaccgcc ggccctgagg gtaaatcgcc
     2881 cgccacgaat cgtgacagtg gtaacgtcat agagggccgg cggcatctga gacgccacga
     2941 atcggctcca gatcagggta tagagggcca gttgatcctt cgtcagaaac tgtcgaagcg
     3001 ataccggatc ccggtacact gaggtcgggc ggatcgcttc gtgggcctcc tgggcgccgc
     3061 ggccgctgcg ataggcaggc ggtcgatccg gcacaaacgg ctgcccgaat cgctcggcaa
     3121 tatagcgggc cgcttcgatc tgggcttcgc tggagacacg aaccgagtcg gtccgcatat
     3181 aggtgatcag tcctacggcg ccctcttcgc cgatctcgat cccttcgtag agttgctgag
     3241 cgatctgcat cgcccgtctg gccgtcaagc gaagcttccg gacggcttcc tgttgcagct
     3301 tgctggtcgt aaacggcgga accgggttgc ggcgcttttc cttcctggtc aggtcggtga
     3361 cccggaactc ttgctccctc agctctcgaa caacctgatc ggtctgcgcc tggtccggca
     3421 ggttcacccg ctcgccgtcg atcttataca accccgccct caatgacgga ggcgttcggc
     3481 cttccaaggc cacatcgacc gaccagtact cggccttgac gaacaccttg atctcccgtt
     3541 ctcgatcgac gataaggcgc agcgcgaccg actggacccg tccggcggac agcccgcgct
     3601 tgaccttatc ccagaggagc ggactgatct ggtagccgac cagacgatcc agaattcgtc
     3661 tggcctgctg agcatcgaca agccgctggt ctatggtgga cggatggttg aatgcctccc
     3721 gcaccgcgcg cttcgtgatc tcgttgaagg tgacgcgatg gaccggttta ttggcggcgc
     3781 cgagctcctg ctgcaggtgc cagcagatgg cctctccctc ccgatccgga tcggccgcaa
     3841 ggtagatggc ttcggcagtc ttggccgcct ttctcaacgc cgacatcacc ttctctcgtc
     3901 cttcgatgat ttcatactgc ggggcgaaat cgcgcgcgat gtcaatgctc agcgacttgg
     3961 ccggcagatc gcggacatgg cccatcgagg ccgttacgac gaactcctta ccgagatatt
     4021 tctcgatcgt tttggccttc gcaggcgact cgacaactac taatgacttt ggcacgctct
     4081 ctgtttcctc cttaaagact agggtaaagg gtatagggga tagggtaaaa gattac
//
LOCUS       DATMXI010000114         4887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_297155, whole genome shotgun sequence.
ACCESSION   DATMXI010000114 DATMXI010000000
VERSION     DATMXI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297155"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VN444_02105"
     CDS             complement(<1..1331)
                     /locus_tag="VN444_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20636.1"
                     /translation="MTTGERPTAVVVNDDLSQLLLMSKLLEKDGLHVRPCRGAEEALT
                     VMCEQGPPDVIVTDLYMPKVDGWRFCRLLRSPEYTAFNRIPILVVSWTFSGADAEQIA
                     ANLGANAFLSAPYEPSELRASVQALLEGRRPQLSMRALIVEDSPMQSKIVSRAFAAHG
                     YTVLTASTGEEARRLFRDHSPQIAILDYHLPDMTADQLLREFKQAAVRMVAIMITANP
                     SPELALQFMRMGADGHVRKPFDPVHLIDLCEKAWRERSLLAVEDLLEERTRQLRERER
                     QYAQLFENANDVIYTHDLSGRFTAFNRMAEQITGYTRSEALTMHVAQVIAPEHLGLHH
                     EITGQNVRGEISGDVCELEIVTRGGHRVPLEVSSGPIYRNGEVVGVQGIARDVSERKR
                     LEAQLRHAQKMEAIGTLAGGVAHDFNNLLTPILGYSRLLKLRGQPGEDVYQA"
     gene            complement(1328..3301)
                     /locus_tag="VN444_02110"
     CDS             complement(1328..3301)
                     /locus_tag="VN444_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20637.1"
                     /translation="MAISETQLDSIFGQEVSRDRSTDSFAMNDHADAIAYLETRLRQQ
                     RQQYQVFQLEHEQLMAAMLGERDARLTEQRTYLERLEQEVQDRTREVRDYAVTLETAN
                     KALEHARAQAEIAARLKSEFLANMSHEIRTPMNGIIGMTELVLDTHLSTEQREYLGLV
                     KSSAESLLGLLNDILDCSKIEAGKLNLEAIPFSLGEILHATLRPLALQAGQKDLPLTW
                     EILPDVLDTLVGDPTRLRQIVVNLVSNAIKFTERGGIAVRAQAIVEADLRQCVLYCSV
                     TDTGIGIPPEKQHVIFDPFIQVDGSTTRQYGGTGLGLTITAHLVTMMGGRIWVESEPG
                     LGSTFHFTARFGVQSPSAKASNGGVAASVIRDRVVPNPAACGHLRILLAEDQVVNRML
                     AVRLLEKAGHTVAVAGTGREVLAALEREQFDLVMMDVQMPEMDGISATAAIRERERRS
                     GAHLPILALTAHAMNGDRERCLAAGMDDYLSKPIRAKALYEAIARLCRSASDLRQDET
                     LTDVTRDGAQAGADSADPSGCRLSSAGADASISLVSGFDREAAMVLVDGDVGLLRRLI
                     CLFLDDCPKRLQEIRDAVRRGDGAALMQVAHSLKGAVGMFGARIAVEALGRLEAMGRD
                     GDVAHAGQTFATMEAALARLTPALAQFMKEGEA"
     gene            complement(3311..4138)
                     /locus_tag="VN444_02115"
     CDS             complement(3311..4138)
                     /locus_tag="VN444_02115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWR20638.1"
                     /translation="MVAQIREISTIPHIALQIIEVVNNPRSSAAALEAVVGSDPSLAV
                     RILRTANSAAYGLRRPVQTVTWAINILGTNEVRNLAMTALVAERFKKDAVIGSYTRRG
                     LWRHMVCVALASRLVAARAGLRTFSEAFLAGLLHDVGIVLLDQYLYQPFAGIVTALKP
                     GISLCQAEREQLGFDHAQLGARVAEQWRFSPVTLAAIRHHHASERCSDESRLIVQTVE
                     VANFICASKGIRSMGASHIAPPSASTLATLSIDRIGLKVLYDDMDGEMAKAHALINI"
     gene            complement(4529..>4887)
                     /locus_tag="VN444_02120"
     CDS             complement(4529..>4887)
                     /locus_tag="VN444_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20639.1"
                     /translation="PGWLKRLLTARDRNPDLAMVGPAMNDGLVPQQVKADYRGTGKAL
                     RRFGVRRAHQYGKQLAVTESLAPSCIVFNPAVCRSIGLLRKDLDLAVSLTDYFARIKQ
                     AGYAVAVALDAYVHCE"
BASE COUNT          986 a         1554 c         1389 g          958 t
ORIGIN      
        1 gcctgataga cgtcctcacc gggctgcccg cgcaacttca gcagcctgga ataacctaaa
       61 atcggggtta acaggttgtt aaaatcgtgc gcgacgccgc ccgccagcgt gcctatcgcc
      121 tccatctttt gtgcgtgtcg gagctgcgcc tccagacgct tccgctcgct gacgtctctg
      181 gcgattccct gcaccccgac cacctccccg ttccggtaga tcggcccgct gctcacttcg
      241 agcggcacgc gatgcccgcc cctggtgaca atctccagct cacagacgtc ccccgatatc
      301 tcaccgcgca cattctgccc tgtgatttcg tgatggagcc ccaggtgctc cggggcgatg
      361 acctgggcga catgcatagt gagcgcttca ctgcgtgtgt agcccgtaat ctgttcggcc
      421 atccggttga acgcggtaaa gcgtccgctc aggtcgtgcg tatagatgac gtcgttggcg
      481 ttctcgaaca actgggcgta ctgccgctcc cgctcgcgca gttgccgagt ccgctcctcc
      541 aacagatctt caaccgccag gagggaccgt tcgcgccacg ccttctcgca gaggtcgatc
      601 aggtgcacag gatcgaacgg cttacgcacg tgcccatccg cccccatccg catgaactgc
      661 agggccaact ccggactcgg attggcggtg atcatgatcg ccaccatccg tacggctgcc
      721 tgcttaaact ccctgagcag ttgatcggcg gtcatatcag gtagatggta gtcgaggatt
      781 gctatctgcg gagaatggtc gcggaagaga cgccgggcct cctcgccggt agacgccgtc
      841 aagaccgtgt agccatgcgc cgcaaaggcg cgactgacga tcttggactg catcgggcta
      901 tcttcgacga tcagcgcacg catcgacagt tggggcctgc gcccttccag cagggcttgc
      961 acgctggccc gcaactcgga gggttcatag ggggccgaca aaaaggcatt cgcgccaagg
     1021 ttggcagcaa tctgctcggc gtcggcaccg gagaacgtcc aggagacgac cagaatcggg
     1081 atccggttga aggccgtata ttccggagac ctcagcagtc ggcagaaacg ccagccatcg
     1141 accttaggca tatacagatc cgtcacgatg acgtctggcg gcccctgttc acacataaca
     1201 gtaagtgcct cttcggcgcc cctgcatggg cggacgtgca gtccgtcctt ctccagaagc
     1261 ttcgacatga gcagtaattg cgacagatca tcattgacga cgacggcggt gggacgttca
     1321 ccggtggtca tgcctctccc tccttcatga attgggcgag cgccggggta agccgcgcga
     1381 gtgcggcctc catggtcgcg aacgtctgcc cggcgtgagc cacatctccg tcacgaccca
     1441 tcgcttccaa ccgcccgagc gcctcaacgg caatccgcgc gccgaacatg ccgactgcgc
     1501 ccttcaagct atgagcgacc tgcataagcg ccgcgccatc accacgtctg acagcatcgc
     1561 gaatctcctg aagccgcttc gggcagtcat cgaggaacag acatatcagc ctccgcagca
     1621 ggcccacgtc accatcgacg agaaccatgg cagcctcccg gtcgaagcct gacacgagcg
     1681 agattgacgc gtccgcccct gccgacgaga gtcggcaacc cgaaggatcc gccgagtcgg
     1741 cgcccgcctg cgctccgtct cgggtcacat cggtgagagt ctcgtcctga cgcaggtcag
     1801 acgccgatct gcacagacgc gcaatagcct cgtagagcgc cttggcccgg atcggctttg
     1861 agaggtaatc gtccatccct gccgccaagc agcgctctcg gtcgccattc atggcatgag
     1921 cagtgagcgc gagaatcggc agatgagcgc cacttcttcg ttcacgctcg cggatggcgg
     1981 cagtggctga gatgccatcc atttccggca tctgtacgtc catcatcacc aggtcgaact
     2041 gctcccgctc cagggcagcc aatacctctc gaccggtgcc ggccacagcc acggtgtgcc
     2101 ccgccttctc cagcagtcga accgccagca tccggttcac cacttggtct tcagccagga
     2161 gaatacggag gtggccacac gcagccgggt ttgggaccac ccgatcccgg attacgctgg
     2221 ccgccacgcc accgttcgaa gccttggctg aaggcgactg cacgccgaat cgcgcggtaa
     2281 aatggaatgt gctccccaac cccggctcac tctcaaccca tatccggccg cccatcattg
     2341 tgaccaggtg ggccgtaatg gtcagaccca aaccggtccc gccgtactgg cgtgtcgtcg
     2401 aaccgtccac ctggatgaag ggatcgaaga ttacgtgttg tttctcaggc gggatgccga
     2461 tccccgtgtc ggtcaccgag cagtacagca cacattgtct aagatcagct tctacaatgg
     2521 cctgtgcgcg gactgctatt ccgcctcgtt cggtaaactt gatggcattg ctgaccaggt
     2581 tgaccacaat ctggcgcaag cgtgtcggat cgcccaccag cgtatccagt acgtcaggca
     2641 gaatctccca ggtaagcggc agatcctttt ggccggcctg cagcgcgagc ggccggagcg
     2701 tcgcatgaag aatctcgccc aggctgaacg ggatagcctc caggttgagc ttgcccgcct
     2761 cgatctttga acaatcaagg atgtcgttca acagaccgag aagcgactcg gcggaactct
     2821 tcaccaggcc aaggtactcc cgctgctccg tactcagatg cgtgtccagt acgagttccg
     2881 tcatcccgat aatgccgttc atcggcgtcc ggatctcatg cgacatattc gcgagaaact
     2941 ccgatttgag gcgggcagcg atttccgcct gggctctggc gtgttccagc gctttattgg
     3001 cggtctcgag cgtcacagcg taatcacgca cctctcgggt gcgatcctgg acctcttgtt
     3061 caaggcgctc caggtaggtt cgttgctccg tcaatcgagc gtcgcgctct cccagcatcg
     3121 cagccataag ttgctcatgc tcaagctgga agacctgata ctgttgcctc tgttgccgaa
     3181 ggcgtgtctc gagataggct atggcatcag cgtgatcgtt catcgcgaaa ctgtcggtcg
     3241 accggtctcg gctcacctcc tgaccaaaga tgctgtcaag ttgtgtttcg gaaatagcca
     3301 cggtgtatcc tcagatgttg atcagggcgt gagccttcgc catctcgccg tccatatcgt
     3361 catataagac ctttaaacct atccggtcga tcgacaacgt agccagggtt gaggcactcg
     3421 gaggggcaat atgagaggcg cccatcgaac gaatgccctt tgacgcgcag atgaagttgg
     3481 cgacctctac tgtctgcacg atcaaccggg attcatcact gcaacgctca gatgcatggt
     3541 gatggcggat cgcagccagg gtgaccggtg aaaagcgcca ttgctcggcc actcttgccc
     3601 ccaattgcgc gtgatcgaag ccaagctgct cacgctcggc ctgacaaagg gatatgccgg
     3661 gcttgagggc cgtcacgatc ccggcaaatg gctgatagag gtactgatcc agcaggacga
     3721 tgcccacatc gtgaaggaga cctgccagaa aggcttcgct gaatgttcgt aacccggcgc
     3781 gcgcggcgac cagacgcgac gccaaggcca cacagaccat atgtcgccac aaccccctgc
     3841 gggtatacga tccgatcact gcgtctttct taaagcgctc ggcaaccaac gcggtcatgg
     3901 ccaggtttct gacctcgttg gtacccaaga tattgatggc ccatgtcacg gtctgaacgg
     3961 ggcggcgaag accgtaggcc gccgagttcg ccgtacgcag gattcgaacc gccagggagg
     4021 gatcgcttcc gaccaccgcc tcaagggcgg cggcggaaga tcgcgggttg tttaccacct
     4081 caatgatctg aagggcgata tgcgggattg tggagatctc gcggatctgc gcaaccatct
     4141 cctctaatga tggtgcggac gtgttaaccg ttgctccgaa agcggtcaat gtggtcgctc
     4201 cctctcctga atctgcgttc tgttgtcgcc ccgccaaccc cgcacccctc ggcgttctcc
     4261 agacaagcgc gataaacctc ctggtaaggg gtaatcagga ctatgacccg acgaatatat
     4321 tatcgtgccg gcaggccgat ttctttaggg gaatctgacg cggctcactt ccccacactc
     4381 acctcatggg gtatcgagga gcaaaggcgt tgacactctg caagccggtg cacacatggc
     4441 gattcgccga aacgcttcca actcacaaac agtgactcgc atgttgccat tgggcaggac
     4501 aaacaggctt gagattcaag cgcgggcttt attcacaatg cacatacgca tccagcgcca
     4561 cagccacggc atagccggcc tgtttaatcc tggcgaagta gtcggtgagt gagactgcga
     4621 ggtccagatc tttacgaagc agcccaatag accggcacac cgccggatta aagacgatgc
     4681 aagagggggc aagactctcc gtcacggcaa gttgtttccc gtattggtgg gcccggcgca
     4741 cgccgaaccg ccgaagcgcc ttcccggtcc ctctgtagtc ggccttgacc tgctggggga
     4801 ccagaccatc gttcatcgca ggaccgacca tggcgaggtc cgggttgcga tcacgggcgg
     4861 tcagcaggcg cttcaaccac cctggtg
//
LOCUS       DATMXI010000115         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_258619, whole genome shotgun sequence.
ACCESSION   DATMXI010000115 DATMXI010000000
VERSION     DATMXI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258619"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VN444_02125"
     CDS             complement(<1..519)
                     /locus_tag="VN444_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWR20640.1"
                     /translation="MKAVIMAGGFGTRLRPLTISISKPMIPMATKPMMEYIVALLKDH
                     GFDDLITLLYFQPEVIERYFGDGSEFGVKMVYAAATEDYGTAGAVKNAEAFLDDTFLI
                     ISGDLLTDFDLTEAVKFHKDRGALATIVLTRVENPLQYGVVITSHDGRITHFLEKPSW
                     SEVFSDTINTGIY"
     gene            1316..1603
                     /gene="groES"
                     /locus_tag="VN444_02130"
     CDS             1316..1603
                     /gene="groES"
                     /locus_tag="VN444_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWR20641.1"
                     /translation="MKVKPLHDRILVKRLEEKEIRKGGIIIPDSAKEKPQEGEVIAVG
                     PGKVGDDGKRQPMDVKAGDKILFGKYSGSEVKIDDKELLIMREEDVLCILQ"
     gene            1704..>2563
                     /gene="groEL"
                     /locus_tag="VN444_02135"
     CDS             1704..>2563
                     /gene="groEL"
                     /locus_tag="VN444_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWR20642.1"
                     /translation="MPAKQLLFDEEARRKIQKGVDLLARAVKVTLGPKGRNVVIDKKF
                     GAPNITKDGVTVAKEIELEDSFENMGAQMVKEVASKTSDIAGDGTTTATVLAQAIFRE
                     GIRNVTAGANPMALKRGIEKAVEGVVEELKKISKPTKGKKEISQVATISANNDKTIGD
                     LIADAMEKVGKDGVITVEEAKSMETTLDVVEGMQFDRGYTSPYFVTDAERMEVVLENP
                     LILIHEKKISNLKDLLPILEQIAKMGKPLMVIAEEVEGEALATLVVNKLRGTLTCAAV
                     KAPGFGDRRK"
BASE COUNT          624 a          608 c          787 g          544 t
ORIGIN      
        1 atagatgccg gtattgatgg tgtcgctaaa gacctcgctc caactcggct tctccagaaa
       61 gtgtgtgatg cggccgtcgt gagacgtgat gaccacgccg tactggagcg ggttctcgac
      121 cctggttaag acgatggtgg ccaaggcacc gcgatccttg tgaaacttga ccgcctccgt
      181 gagatcgaaa tcagtgagga gatcgccgct gatgatcagg aaggtatcgt cgagaaaggc
      241 ttcggcgttc ttgaccgctc cggcggtgcc gtagtcctcg gtggcagcgg catacaccat
      301 cttgacgccg aattcgctac cgtcgccgaa gtagcgctcg atgacctctg gctggaagta
      361 gaggagggtg atgagatcgt caaagccgtg atccttgagg agcgccacaa tgtactccat
      421 cattggcttg gtggccattg ggatcatcgg cttggagatg ctaatcgtta gcggacgcag
      481 tcgcgttccg aatccgccgg ccatgataac ggctttcatg cgctttcctc tgagtttata
      541 tgcaactcgc ccgggaactg gcgtctcttt atcgtaggag tataggttcc agcgcggaat
      601 actgtcaaga ccattatcag ccaaaacgaa gcattaggcg cgtgggtaga tcaaggtctc
      661 tgcggtagtg gaatgaagaa tggatagaac cgcttcagcg cattgcatgc gagcgagtag
      721 ctgcaaggtg tttaggctga aggctattag tctttgcgca cggaccgact caaggcgccc
      781 agaacctttt cagcctccat tattgtcgcg tcgcactcgg aaaacgcctg gctcatctgt
      841 gcgcgttcct acagtctaaa agcctacagt ctatctatct aagtggttac gagagcgaga
      901 gttttgcgag cgggttttcg cctgaagctg cattgatctg acttataggg gtgactcctt
      961 cgggcccttg gtcgaaccta aaagacagat caccataaag aacatctgca tatttcagcg
     1021 ataaacagct caataattga tttaacctct tctaatggcg attatttcca actttcatcc
     1081 gatatatccg atcgtttggc cttgcgaatg agggtcaaag cgcctatact gccttagcac
     1141 tcacttcatg agagtgctaa cagaatcgtt gcgagggaga gatcgttgtt gggtgcgttg
     1201 aggtaaaccg tattgcctgt ctgcgtgtat cgcactggca gggagttgcc ggagcacatc
     1261 tgctccgcgc ctgcctgtcg gcagatagca gacagggaga aaggagtaag ggatcgtgaa
     1321 ggtgaagcca ctgcacgacc gaatcctggt gaagcgtctg gaagagaaag agatcaggaa
     1381 gggcgggatc atcattcccg acagtgcgaa agagaaacct caagagggtg aagtcattgc
     1441 cgttggccca ggtaaggtcg gcgatgatgg gaaaagacag ccgatggatg tgaaggccgg
     1501 cgataagatc ctctttggca agtactccgg ctcggaggtg aagatcgacg acaaggaatt
     1561 gctgatcatg cgggaggaag acgttctatg catcctgcaa tagcaggttt gtaggacgtt
     1621 gacgacgaga tcgccgtttc gcggttaaac cttgtccccg actccgatcg gggaactgat
     1681 acagattagg gaggaaagct gatatgccag cgaagcaatt actgttcgat gaggaggcaa
     1741 ggcgaaagat tcaaaaaggt gttgacctcc ttgcccgtgc cgtgaaggtg accctgggcc
     1801 ccaaggggcg caatgtggtg atcgacaaga agtttggcgc cccgaatatt acgaaggacg
     1861 gggtcacggt ggccaaggag atcgagcttg aggatagctt tgagaatatg ggcgcccaga
     1921 tggtgaagga ggtggcgagc aagacctctg atatagccgg agacggcact accaccgcca
     1981 ctgtattggc tcaggcgatc ttccgggagg ggatcaggaa cgtgacggcc ggcgcgaacc
     2041 caatggcgct gaagcgggga atcgagaagg ccgttgaggg cgtcgtagag gagctgaaga
     2101 aaatctccaa gccgaccaag gggaagaagg agatctccca ggtcgccacc atctcggcca
     2161 acaatgacaa gacgatcggc gaccttatcg ccgatgccat ggagaaggtc ggtaaggacg
     2221 gggtcatcac cgtcgaagaa gccaagagta tggaaacgac tctcgatgtg gttgagggga
     2281 tgcagtttga ccgaggttac acctcgcctt acttcgtaac cgatgctgag cggatggagg
     2341 tggtactgga aaaccctctg atcctgatcc acgaaaagaa gatcagcaac ctcaaggatc
     2401 tcctgccgat tctggagcag atcgccaaga tgggcaagcc gctgatggtg attgctgaag
     2461 aggttgaggg cgaggcgctg gccaccctgg tggtcaacaa gttgcgtggg accctgacct
     2521 gtgcggcggt taaggctcca ggctttggcg accggcgcaa gga
//
LOCUS       DATMXI010000116         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_323519, whole genome shotgun sequence.
ACCESSION   DATMXI010000116 DATMXI010000000
VERSION     DATMXI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323519"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="murG"
                     /locus_tag="VN444_02140"
     CDS             complement(<1..872)
                     /gene="murG"
                     /locus_tag="VN444_02140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWR20643.1"
                     /translation="MKAIIAGGGTGGHLFPAIALAEELRSRRPDLSLLFVGVEGGFEA
                     SLLATRGWDFEGIRASGLQGKRLLSRLRSLTLIPSGLIRSLSILRRFRPDVVVGFGGY
                     ASAAMVLSGVLARVPTVIHEQNALPGLANRWLGRIVDRVAVAFEEASEFFPKSKVRVT
                     GNPVRAELFGVSRAEAATRLGLDPNRFTLLIFGGSQGAHRLNQAVMEALPMLTDERER
                     IQFIHATGPRDLSLVRQGYDALGCPAVVEPFFQAMAVAYATADLCFCRAGAGTVAELC
                     ALGKPSVLVPFPFAA"
     gene            complement(869..1984)
                     /gene="ftsW"
                     /locus_tag="VN444_02145"
     CDS             complement(869..1984)
                     /gene="ftsW"
                     /locus_tag="VN444_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWR20644.1"
                     /translation="MITKRLSYDRLLCGVALLLVGLGIVMVYSASAIKAQDKYGDPFF
                     FLKKQILWALIGFAVMVWAMYRDYRIFQRYAPLLFFVSLFLLILVLTPSIGVKVNSAR
                     RWLRLFGVSFQPSELARLSIVLLTARLLARSADREESFAKRFLKPLILSGLVCGLIVF
                     QPHIGMAAVLMCAVLALCFVAGVRLSHLSIVALVVVTLASLFVLTQPYARTRVMTLLD
                     PNHASSQAAHQTHQSIYALGPGGLLGRGLGDSFGKLGYLPESHTEFIFAVVGEETGFV
                     GALLVVFLFGILLWRGTRIALRAPDLFGAYAAMGITFTIVTQAAINFGVVVGLLPITG
                     LPLPLISFGGTSLITTCLCIGILLSISRSQTARGRLA"
     gene            complement(1981..>2328)
                     /locus_tag="VN444_02150"
     CDS             complement(1981..>2328)
                     /locus_tag="VN444_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWR20645.1"
                     /translation="FTNPVVLIAGGRDKHGDFAPLIPLVRERVKRLILIGEAAETLRR
                     TLALACPTEDASSLEEAVQRAAAAASPGEVVLLSPACASFDMFTDFEERGRAFKAAVQ
                     ELLPPAYSIRGHA"
BASE COUNT          551 a          729 c          688 g          360 t
ORIGIN      
        1 gcggcaaagg ggaacggaac gagcaccgac ggcttcccca gggcgcagag ctctgccaca
       61 gtgccggcgc cggcccgaca gaaacagaga tcggcggtgg catacgccac ggccatcgcc
      121 tgaaagaacg gctctaccac cgcggggcac ccaagagcgt catacccttg ccggacaaga
      181 gaaaggtcgc gcggacccgt ggcatggatg aactggatcc gctctcgctc atctgtaagc
      241 atggggagcg cctccatgac cgcctggttc agccggtgag ccccctggct gccgccgaag
      301 atcagaaggg taaatcgatt tggatcgaga cccaagcggg tcgctgcctc tgccctgctc
      361 acgccgaaga gctccgctcg gactggattg ccggtcaccc gcaccttact cttggggaaa
      421 aactcggatg cctcttcgaa ggcaactgcg acgcgatcaa cgatcctccc caaccatcgg
      481 ttagcaagcc ctggcaaggc gttctgttca tggatcacgg taggaaccct cgcaagcact
      541 cccgacagta ccatggcagc cgaggcgtag ccaccaaacc cgaccacaac atcgggacgg
      601 aaccgtcgaa ggatcgatag agaccggatg aggcctgagg ggatcagcgt aaggctccga
      661 agacgcgaca ggagccgctt cccctgtagg ccggaagccc tgatcccctc gaagtcccaa
      721 cctctcgtag ccagcaaaga tgcctcaaac cctccctcga cgccaacaaa taggagcgac
      781 aggtccggcc ttcttgacct gagctcctca gctaacgcaa tggcaggaaa gaggtgaccg
      841 ccagtcccgc ctcctgcaat gatggccttc acgcgagtcg tcctctcgcc gtctgggaac
      901 gagagatgct gagcaggatc ccaatacaca agcaggtggt gatcagggat gtgccgccga
      961 agctgatcaa cggcaaaggc aaacccgtga tgggtagcag tccgacaacc accccaaagt
     1021 tgatggcggc ctgtgtcacg atggtaaagg taattcccat cgccgcgtag gcaccgaaca
     1081 gatcaggcgc tcgaagtgcg atccgggtcc cgcgccacag gagaataccg aagaggaaga
     1141 ccaccaacag agcccccacg aatcctgtct cctccccgac gacggcaaag atgaactcag
     1201 tatgggactc gggcagataa cccagcttcc caaagctgtc accgagcccc cgtcccagaa
     1261 gtccccccgg tcccaatgcg tagattgact ggtgggtctg gtgcgctgct tgggaggagg
     1321 cgtgattcgg gtccaacagc gtcatcactc gcgttctggc atacggctgg gtcaggacga
     1381 acagggaggc aagcgtcacg accacgagag caacaatgct cagatggctg agacggacgc
     1441 ctgccacgaa acacagtgcc aatactgcac acatcaggac cgccgccatc ccaatgtgtg
     1501 gctgaaacac aatcaggccg cacacgagtc cggagaggat aagcggtttg agaaagcgtt
     1561 tggcgaagga ctcctcccga tcggcgcttc tggcgaggag cctggccgtc agtagaacga
     1621 tggagagtct ggcaagctcg gaaggttgaa aggagacccc gaacagccgg agccatcgcc
     1681 gggcgctatt gaccttgacc cctattgacg gggtcagcac caggatgagg aggaacagcg
     1741 agacgaagaa taacaggggc gcataccgtt gaaatatccg gtagtcgcgg tacatggccc
     1801 agaccatgac cgcgaacccg atcaaggccc agaggatctg cttcttcagg aaaaagaatg
     1861 ggtcgccgta tttatcctgg gccttgatgg cgctcgcgct atagaccatg acgataccga
     1921 gtccgaccaa caacagcgcg acaccgcaca gcagcctatc ataggagagg cgcttcgtga
     1981 tcatgcgtgc ccccgtatgc tgtaagcggg aggcagaagc tcctgtaccg ccgccttaaa
     2041 cgcgcgcccc cgctcttcaa aatcggtaaa catatcgaag ctggcgcagg ccggcgaaag
     2101 cagcacaacc tctcctggcg atgcggcggc tgcggcgcgc tgcactgcct cctcgagact
     2161 ggacgcatcc tccgtcggac acgcgagggc cagcgttcgc cgcagcgtct cagcggcttc
     2221 accgatcagg atcagccgtt tgacccgctc gcggaccagc gggatgagcg gcgcgaaatc
     2281 gccatgcttg tccctaccgc cggcgatgag cacaacgggg ttcgtaaa
//
LOCUS       DATMXI010000117         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_145346, whole genome shotgun sequence.
ACCESSION   DATMXI010000117 DATMXI010000000
VERSION     DATMXI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145346"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /gene="lptE"
                     /locus_tag="VN444_02155"
     CDS             complement(<1..502)
                     /gene="lptE"
                     /locus_tag="VN444_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWR20646.1"
                     /translation="MVRPETKNGAIRNEGAYRLFMLTAHVSALCLFLVLLGACGYQFR
                     VEGAGPTIGGAAAALASTSPPPRLVVRTLENRTFEPNLETRYTNYLRHEFSSGSGAQV
                     VSDSEAADLVLSGQILSVLLPTLSFTQTTTLESRAEVVVIVKVEETRTQRLVWAQTAK
                     GASEFFV"
     gene            complement(514..>2342)
                     /gene="leuS"
                     /locus_tag="VN444_02160"
     CDS             complement(514..>2342)
                     /gene="leuS"
                     /locus_tag="VN444_02160"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWR20647.1"
                     /translation="LEDLKLVEWPASTLEMQRNWIGRSIGAEVEFDLAGVPGTVRIFT
                     TRPDTVFGATYMVLAPEHPLVAVVTATDRRTEVTAYREAAARKSDLQRQELEKEKTGV
                     FTGGYALNPVNKEPLPIWIADYVLMGYGTGAIMAVPAHDERDWVFAKTYGLPIREVIA
                     GGRIEQEAFVATDKGTVINSTTPDRSFSIDGLTPEDAIPKITAWLESQGKGRKAVNYK
                     LRDWLFARQRYWGEPFPIVWVDGQAVPLPEEQLPILLPETNNFKPSGTGESPLANLTE
                     WLTTTDPISGAAARRETNTMPQWAGSCWYYLRFIDPKNQKQLVDPAQERYWMPVDLYI
                     GGSEHAVLHLLYARFWHKVLYDIGVVSTPEPFRKLVHQGIVLGEDNQKMSKSRGNVVN
                     PDEMMDQFGADAVRLYEMFMGPLEAVKPWSTRGVEGVTRFLERAWRLMVNEEGRLSSA
                     VTNTVPTLEHQRVLHQTIKKVTEDIEGLRFNTAISQMMVFTNEMTKAEQRSRILLEPF
                     VLLLAPFAPHLAEELWEVLGRQPSVSQQPWPVFDPELIISDRMIIPIQVNGKLRAKLD
                     VGADATREQVEGLARAQAAEWLQGNEPKKVIYVEKKLINFVI"
BASE COUNT          499 a          736 c          603 g          504 t
ORIGIN      
        1 tcacgaaaaa ctccgaggcg cccttcgccg tttgcgccca cacgagacgt tgcgtcctgg
       61 tttcctccac cttaacgatc actacaacct ctgcgcggct ttcgagcgtg gtggtctgag
      121 tgaaactgag cgtgggaaga agaaccgaga ggatttgccc ggacaggacc agatcagccg
      181 cctcagaatc agaaacaacc tgggccccac tgcccgagga aaattcatgc cgcagatagt
      241 tcgtatagcg ggtttccaag ttcggctcaa acgttcggtt ttccaacgtc cggacgacca
      301 accgtggtgg cggtgacgtc gatgcgaggg ccgcagcagc tcctccaata gtgggcccag
      361 ctccctccac acggaattga tagccgcagg cccctagaag cacaaggaac aggcagagtg
      421 ctgagacgtg agcggtgagc atgaaaaggc ggtacgcccc ctcgttccga atcgcaccat
      481 tcttggtctc aggtcttacc actccgcact tccttagatg acaaagttta tgagcttttt
      541 ctccacgtag atcacttttt tgggttcgtt accctgcaac cactccgcag cctgggctcg
      601 agccagccct tcgacctgtt cgcgagtggc gtcagcgccc acatcaagtt tggcccgcaa
      661 ttttccattc acctggatcg gaataatcat ccgatcactg atgatcagct caggatcgaa
      721 gaccggccaa ggctgttgag agacactcgg ctggcgaccc agcacctccc atagttcttc
      781 cgccagatgg ggagcgaagg gcgcaagaag aagaacgaaa ggttcaagca agatccgaga
      841 gcgctgctcg gccttcgtca tctcgttcgt aaacaccatc atctgggaga tcgccgtatt
      901 gaaccgcaat ccttcgatgt cctcggtcac tttcttgatc gtctggtgga gcacacgctg
      961 gtgttcgaga gtggggactg tgttcgtcac ggcactcgac aatcggccct cttcattcac
     1021 catgagccgc catgcccgtt cgagaaatcg cgtcacccct tccacacccc tcgtactcca
     1081 aggcttcacg gcctccagcg gacccatgaa catttcatag agccgcaccg catcggcccc
     1141 gaactggtcc atcatctcat cggggttcac gacattgccg cgggatttcg acatcttctg
     1201 gttgtcttct cctaacacga ttccctgatg cacaagtttc ctaaacggct ccggcgtgct
     1261 gacaactccg atgtcgtaga gaaccttgtg ccaaaaccgc gcgtagagca aatgcagcac
     1321 ggcatgttcg ctgccaccga tatagagatc caccggcatc caatagcgct cctgtgcagg
     1381 atcaacgagc tgcttctgat tcttcggatc gataaaccgt aaataatacc aacaggagcc
     1441 ggcccactga ggcatcgtgt tggtttcacg gcgtgccgct gccccgctga tcgggtcggt
     1501 ggtcgtcagc cattcagtga gattcgcgag cgggctttcg ccggtcccgg acggtttgaa
     1561 attgttcgtt tccggcaaca atatcggcaa ctgctcttca ggaagcggga cggcctggcc
     1621 atctacccac acaatcggaa agggctcgcc ccaataccgc tggcgcgcaa atagccaatc
     1681 ccgcaattta tagttcacgg ccttgcggcc cttgccctgc gattctaacc aggccgtgat
     1741 cttcggaatc gcatcctccg gtgtcagccc atcaatggaa aagcttctat ccggcgtcgt
     1801 ggaattgatt accgtcccct tatcggtcgc aacgaacgcc tcttgttcaa tccgaccacc
     1861 cgcgatgact tcacggatcg gcaacccata ggtcttcgca aaaacccagt cgcgctcgtc
     1921 gtgcgcaggc acagccatga tggccccggt cccatacccc atcaacacgt aatcggcgat
     1981 ccaaatgggc agtggttcct tgttcaccgg attcaatgca tagccgccgg tgaagactcc
     2041 agtcttttct ttttcaagct cctgccgttg gaggtcgctt tttctcgccg ctgcctcgcg
     2101 ataggcggtg acctcagtgc ggcgatcggt ggccgtgacg accgcaacta acggatgctc
     2161 cggagccagc accatatagg tcgcgccgaa cacggtatcg ggccgcgtcg taaagatccg
     2221 aaccgtgccc gggacgccgg ccagatcgaa ctcgacttcc gcgccgatcg atcggccgat
     2281 ccaatttctc tgcatctcca acgtgctggc cggccattcg accagcttca agtcttcgag
     2341 ca
//
LOCUS       DATMXI010000118         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_273036, whole genome shotgun sequence.
ACCESSION   DATMXI010000118 DATMXI010000000
VERSION     DATMXI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273036"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1621
                     /gene="trpE"
                     /locus_tag="VN444_02165"
     CDS             125..1621
                     /gene="trpE"
                     /locus_tag="VN444_02165"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWR20648.1"
                     /translation="MAPPEYSLSLDQLWQYAGEGNLVPIYREILADYETPVSAFAKID
                     HGPSAYLLESIEGGEKWARYSFLGSGSPVVVYEDGGDLLVVQGTSKKRIPSRGNPLER
                     LRELMEAYRPVTVPGLPPFVGGAVGYLSYDMVRTFEDLATRKKDPLNLPDFAFLLTDT
                     MLIFDNVVQKIKVVANAHVTAKGERGIKTAYRSATERIERTIARLKRPLRRTRPHRRR
                     RPVSFTANMSKADFEKMVVRTKEYIRAGDIVQAVLSQRWETSVQASPLQLYRALRVVN
                     PSPYMYYLRIAGVELVGSSPETLVRCENGVISVRPIAGTRRRGATPEEDQQMERRLLA
                     DKKERAEHVMLVDLGRNDVGRVAKPGSVVVDSLMHVERYSHVMHIVSNVTGQLDEAKT
                     SYDVLRACFPAGTVSGAPKVRAMQIIDELEPTRRGPYAGAVGYFSFSGNMDMCINIRT
                     VVVKKHQAFIQAGAGIVADSNPEHEYEETCNKAKAMMKAVELAEQGLE"
     gene            1713..>2203
                     /locus_tag="VN444_02170"
     CDS             1713..>2203
                     /locus_tag="VN444_02170"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWR20649.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDIRVFRNDKITIDQIESLQP
                     SRIVISPGPCTPVEAGVSVETIRHFGGRLPLLGVCLGHQSLAVAFGGEVVRAERLMHG
                     KTSKIRHDEKTIFHQLPNPFDATRYHSLIVKRETLPSCFEISAETAEGEIMGLRHRSL
                     GIEG"
BASE COUNT          490 a          585 c          644 g          484 t
ORIGIN      
        1 ctatcggatc agtcggcaat atggagtcac ggtcgagaag gtcagaaagt ggaacaagtt
       61 gtctgacgac attatcgagg taggacagaa gctcatcgtg ggccaagaat aataggctga
      121 ccacgtggcc ccgccagaat actcactcag tttggaccaa ctttggcagt acgccggaga
      181 aggcaatctc gttccgatct atcgagagat tcttgcggac tatgaaaccc ccgtttctgc
      241 ctttgccaaa atcgatcatg gcccttcagc ctacctccta gagtccatcg agggcggcga
      301 gaagtgggct cgctattcct ttcttggaag tgggtcgcct gttgtcgtgt acgaggatgg
      361 aggcgacctg ctggtcgtgc aaggcaccag caagaagcgc attcccagcc gtggaaaccc
      421 tctcgaacgt ctgcgggagc tgatggaggc ctatcgtccg gtaaccgtac cggggctacc
      481 cccctttgtc ggcggcgcag tcggttatct gagctacgac atggtgcgca ccttcgagga
      541 cttggctacc cggaagaaag accctctcaa tctgccggat tttgccttcc tccttaccga
      601 tacgatgctg atcttcgaca atgttgtgca gaagattaaa gtcgtggcta acgcccatgt
      661 gactgcaaag ggggaacgcg gtatcaagac cgcctaccga tcggcgacag aacggatcga
      721 acgcacgata gcaagactca aacgtccgct ccgccgaacg cgtccacatc gccgccgccg
      781 accagtgtcc tttacggcga acatgagcaa ggcggatttt gagaagatgg tggtacggac
      841 gaaggaatac attcgggccg gtgatatcgt gcaagcagtg ttatcccagc ggtgggaaac
      901 gagcgtgcag gcatcgccgt tgcaactgta tcgggcgttg cgcgtagtga atccctctcc
      961 ctatatgtat tacctgcgca tcgccggggt cgaattggtc ggctcctctc cagagacgct
     1021 cgtccgttgt gaaaacggcg tcatttccgt ccgtcctatt gcgggcacgc gccggcgtgg
     1081 tgcgacgcct gaagaagatc aacagatgga gcggcggtta ttggcggata aaaaggaacg
     1141 ggctgagcat gtgatgctcg tggatcttgg gcggaatgat gtcggtcgtg tggcgaaacc
     1201 aggctcggtc gttgtggact cgctcatgca cgtcgagcgg tattcacacg tgatgcacat
     1261 tgtgtcgaat gtgacggggc agcttgatga agcgaagacc tcgtacgacg tgttgcgcgc
     1321 ctgctttccc gccggcaccg tttccggcgc gccgaaggtg cgagcgatgc agatcatcga
     1381 tgaactcgag ccgacaagac gcggtcccta tgccggagcg gtgggctact ttagcttttc
     1441 tggtaacatg gatatgtgca tcaatatcag gaccgttgtc gtgaaaaaac accaggcctt
     1501 cattcaagca ggggccggta tcgtcgcaga ttcgaatcca gaacacgagt atgaagagac
     1561 ctgcaataag gccaaggcca tgatgaaggc ggtcgaattg gccgaacagg ggctggaatg
     1621 agcacgtaag gcgtgagtgg taagtggtga agtgatcagg atctcgccca cttttcccct
     1681 tacgcctgac gcctaccacc tcacggttta ttatgttgct gatgatcgac aactacgatt
     1741 ctttcaccta taacctcgtg caatacttcg gggaactcgg tgaagacatt cgggtgttcc
     1801 gtaacgataa gattacgatc gatcagatcg agtcgctgca acccagccgc atcgtcattt
     1861 ctcccgggcc ctgcacgccg gtggaagctg gtgtatcggt cgagaccatc cggcattttg
     1921 gcgggcgcct tcctctcttg ggtgtctgtc taggccatca atctctggct gtggcgtttg
     1981 gcggggaagt cgtccgggcc gaacgattga tgcatggcaa aacctcgaag atccgccatg
     2041 acgagaaaac gatcttccac cagctgccga atcccttcga cgcgacacgc taccattctc
     2101 tcatcgtgaa gcgagagacg ctgccttctt gttttgagat ctccgccgaa actgccgaag
     2161 gggaaattat gggattgcgc catcgatcgt taggcatcga agg
//
LOCUS       DATMXI010000119         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_126785, whole genome shotgun sequence.
ACCESSION   DATMXI010000119 DATMXI010000000
VERSION     DATMXI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126785"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..602)
                     /locus_tag="VN444_02175"
     CDS             complement(192..602)
                     /locus_tag="VN444_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20650.1"
                     /translation="MQQLTDAAKITRDYSRKRGLSLLLRTMHLVSFGILLGGHTFAID
                     ADRLLPALYLTIASGGVLMTLEISTIGPHWLFMGKRATVLLKLLPLLAIPYAWDYRLP
                     LLLLVVAIAVGSHMPGRYRHYSILQGRIVQSGRL"
     gene            complement(635..2131)
                     /locus_tag="VN444_02180"
     CDS             complement(635..2131)
                     /locus_tag="VN444_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWR20651.1"
                     /translation="MEQRKWWIRGVVLGSALLLAPVGAWADKLTELEQAFETQQKSLQ
                     QLQQEMHRLRQDRTAQQTDIDRRVMEVEKKAADAAASSLLTGYEGGKGFFLKSADDQF
                     RLNLSGYVQNWMQVEGAKEDEHFPAASVAAGNARHDASTFKMRRARIILSGTIYKDFG
                     FHIEPELVSGGQGTRLEAAFATYTYAPWAKVKVGQFRDMYSLEMATASQDLDFAERAV
                     AVRALAPDLQMGLMFSGNLKLANVLPVYYGLGIFNGCGRVDQCSIDNDGDKEFTGRFA
                     FSPPMPIGNLTIGLNADHRNFDIVRGNGARDLVTAPATSTAHRFNPIGPTGTTLAGNG
                     NGLQNGFLINGDRNTGGVDFVFDWYPFILKGEYHYALQKRNGLDPVNVGKDLDELQMQ
                     GTYGTIGYWIFGNKRNGLLANTRYEYMRVDDRKGHFNSATTGAGTEETLEMQSGTLGL
                     TWYINPSVWVRGNYIVTDLSPNKNQVGMSNSHGSNVAQQGIAELMVKF"
     gene            3039..>3785
                     /locus_tag="VN444_02185"
     CDS             3039..>3785
                     /locus_tag="VN444_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWR20652.1"
                     /translation="MAYPGTGSKRLVGTRRTGRRQDAGVRAGDAVAPRVDEECEYRPV
                     VDEKASQGEPGDTQPSESLKAAFRLFEETADGVFISSPSGEILFCNRAAETILEASAQ
                     QVVGQECREFFNGRDSNGNQLCQWPCPLKMSMGRGDLIQHFEMATRTRTGKPLWIDVS
                     CVALPCDDNQPPTVAHLFRDVTAAHQLEVLVRQQLAQTQLAMSEEIPPHIVELTRREL
                     QVVTLMRTGATTAAIAEQLFISKTTVRNHIQ"
BASE COUNT          771 a         1107 c         1127 g          780 t
ORIGIN      
        1 tattggccgt aggtgacata agcggacttc atcccctcgt aacaggcggc aggaagatcg
       61 ttttccagct tggcccatgc ccccggcctt accttggcta aagtaaggaa cttcatgatt
      121 ggctaccctc cattattcga ctaaggacgg tgaggttgtg tgtccggcgt tctctctcgg
      181 cgaggtcacg cttagagcct ccccgattgg acaatacgac cttgaaggat cgaatagtgg
      241 cggtaccgcc caggcatgtg tgaccccacg gcgatcgcca cgaccagcaa aagaagaggc
      301 aaccgataat cccaggcata gggaatagcc agaagaggca gcagcttgag aagcacggtt
      361 gcccgtttgc ccatgaagag ccagtgtggt ccgatggtgg agatctccag cgtcatcaga
      421 acgcccccgc tcgcgatcgt aaggtacagc gcaggtagca aacggtctgc gtcgatcgcg
      481 aacgtgtgcc cccccagcag gatgccgaag ctcaccagat gcatggttcg tagcagaagc
      541 gacagacccc gctttcgcga ataatcccgc gttatcttcg cagcgtctgt cagttgttgc
      601 atctggtgaa ctccggctga agcctgccat caatttagaa cttcaccatc aactcggcga
      661 tcccttgctg agcaacattg ctgccgtggc tattgctcat tccgacttga ttcttgttag
      721 ggctgaggtc ggttacaatg tagttacccc gcacccagac gctggggttg atataccagg
      781 tcaggccgag ggtgccggac tgcatctcca atgtctcctc ggtgcctgca cctgtcgtgg
      841 cagaattaaa gtggccctta cggtcatcga cgcgcatgta ctcgtagcgc gtattggcca
      901 gcaggccatt gcgcttatta ccgaagatcc agtagccgat cgtcccatac gtaccctgca
      961 tttggagctc atcgagatcc ttgcctacgt tgacagggtc caggccgtta cgcttctgga
     1021 gggcgtagtg gtattcgccc ttgagaataa acgggtacca gtcgaacacg aagtcgacgc
     1081 cgccagtgtt tcggtcgccg ttgatcagga agccgttctg caggccgtta ccgttaccgg
     1141 ccagcgtggt gccggtcggg ccaatcggat taaagcggtg agccgtcgag gtggcgggcg
     1201 ccgtcaccag gtcgcgagcg ccattacctc tcacgatgtc gaagttacgg tgatcggcat
     1261 tcaagccgat ggtgagattc ccgatgggca tcgggggtga aaaggcaaat cgaccggtga
     1321 actccttgtc gccgtcgttg tcgatggagc actgatcgac ccggccgcag ccgttaaaga
     1381 ttcccaatcc atagtagacg ggtagcacgt tggcgagttt cagattgccc gagaacatga
     1441 ggcccatctg caggtccggg gcaagcgccc tgacggcgac cgcccgctcg gcgaagtcca
     1501 ggtcttgaga ggccgtggcc atctcgaggc tgtacatgtc cctgaactgc cctaccttca
     1561 cctttgccca aggggcatac gtgtaggtgg cgaaggccgc ttcgagtcgc gtgccctgtc
     1621 cgccgctcac gagctccggc tcgatgtgga agccgaagtc cttgtagatc gtaccgctca
     1681 agatgatccg ggcgcggcgc atcttaaagg tgctcgcgtc gtgccgtgca ttcccggctg
     1741 ccactgatgc agcggggaaa tgttcgtcct cttttgcccc ttcaacctgc atccagttct
     1801 ggacataccc gctcaggttc aggcggaact ggtcgtcggc tgacttcagg aagaatccct
     1861 tgccgccctc atacccggtg agcagcgacg aggccgcggc gtcggcggcc ttcttctcca
     1921 cctccatcac gcgcctgtcg atatcggtct gctgcgcggt ccggtcttgc cgtagacggt
     1981 gcatctcctg ctgcaactgc tggagcgact tctgctgcgt ctcaaatgcc tgctcgagct
     2041 ccgtgagttt gtcagcccac gcccccaccg gggcgagcag caaagcgctg cccaggacga
     2101 caccgcgaat ccaccacttt ctttgttcca tgactccccc tctgcgtttt aaggttaatc
     2161 accgcactcc cgaattcgga gcgcttttcc ggtgcggggc gctgcagtag ccccgtcccg
     2221 atcacgcaat cccgcgctga gtgaacgcga gcttcagacc ctgaagcgag aatcgaaaca
     2281 tcctatgcaa ctcctgctac atggtgcgac actattgccc agtttgcgcc cccctgtcaa
     2341 ggattttctt cggccggcca ggatggcaca tttcggtcga aggcgatcga cgaaaatggg
     2401 agcgtacatg ccgggcgaca tgcccctacg gtggatagcc gcatggggat gaacgcggag
     2461 agacgggagg cttcagactg atgagtccac agggggtcat tccgttgaga gttgataccc
     2521 cgcggcttgc cgcaagttcg tcataccggc ggaagccggt atccagaggc cccgattgaa
     2581 ttccgtgtca agcacggaat gacgggccag aacagaagac gataccccgc agcttgctgc
     2641 gggggagttc gttggacgcc agagggcgcc gcccggctcc tggagcagcg ccagttgccg
     2701 gcggaaaagg tgtaaatcga gtgtcgcgat gtggcgtccg tggcgcacgc gatccggact
     2761 aagtagatct actatcgaga tcggcgcggc agcacgtacg ccacaagtca gggaagaaga
     2821 tgacacgtga gggaactctg tagtgagctt atcgaaccgc acgctggtgt acttgggcgg
     2881 gaccggaagt agcgccgcct ctataaggcc gtggagctcg tacggatact taccgcagcg
     2941 ggggccaggt tcgggtcgag gactgaaggc gtggatgggc actctgctca tacccttttt
     3001 ggcttgacag gttttactga ggactgtaca ttagcatcat ggcatatcca ggcaccggat
     3061 cgaaacgatt ggtggggacc cggcggacag gccgtaggca ggatgccgga gttcgtgcag
     3121 gtgatgcggt tgccccccgt gttgacgagg aatgcgagta tcggcctgtg gttgatgaaa
     3181 aagcctcgca aggcgagcca ggagacacac agccttcgga atcgctgaag gctgccttca
     3241 ggcttttcga ggagacggcc gatggggtat ttatcagttc accgtcgggt gagattctgt
     3301 tctgcaaccg ggccgctgaa accattctgg aagcttccgc gcaacaagtc gtcgggcagg
     3361 agtgccgcga atttttcaac ggccgtgaca gtaacggcaa tcagctctgc cagtggcctt
     3421 gcccactcaa gatgtctatg ggccgcggag acctgatcca gcattttgag atggctactc
     3481 ggacgaggac aggcaaaccg ctgtggatcg acgtgagctg tgttgccctt ccctgtgacg
     3541 acaaccagcc tccgacggtc gcccacctct tccgcgatgt caccgcggca caccagcttg
     3601 aagtcctcgt tcgccaacaa ctggctcaga ctcaactcgc gatgagcgag gagatccctc
     3661 cccacatcgt tgaactgact cggcgggaac tgcaggtcgt cacccttatg cggactggcg
     3721 caaccacggc ggccatcgcg gagcagcttt tcatcagtaa aaccacggtg agaaaccata
     3781 tccag
//
LOCUS       DATMXI010000120         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_323470, whole genome shotgun sequence.
ACCESSION   DATMXI010000120 DATMXI010000000
VERSION     DATMXI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323470"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..396)
                     /locus_tag="VN444_02190"
     CDS             complement(55..396)
                     /locus_tag="VN444_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWR20653.1"
                     /translation="MAYIVAEPCIGTKDHACVDVCPVECFYEGEEILWIHPEECIDCA
                     ACEPECPVAAIFEATQVPEQWQQYIQMNADACKDGHTLPVAVQRAVWEAQKEEEGSVA
                     NLYYKKYPAKH"
     gene            complement(464..775)
                     /locus_tag="VN444_02195"
     CDS             complement(464..775)
                     /locus_tag="VN444_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWR20654.1"
                     /translation="MNTSEIEAEVIEALRTVYDPEIPVNIYDLGLIYEVKVEPSGLAR
                     VVMTLTSPHCPAAAELPAGVEVKVRCVPGVVDAQVEVVWDPPWDSSKLSEAAKLQLGF
                     L"
     gene            complement(874..1326)
                     /locus_tag="VN444_02200"
     CDS             complement(874..1326)
                     /locus_tag="VN444_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWR20655.1"
                     /translation="MSDLRELYQEVILDHNKRPRNFQKLEGANRAADGYNPLCGDQIT
                     VYLLVEDHVIRDIAFQGSGCAISKASASMMTAAVKGKTTAEAEGLFETFHRMVTADLS
                     AACDPLVVGKLAAFAGVREFPVRVKCATLPWHTLQAALEGRGQISSTE"
     gene            complement(1326..2579)
                     /locus_tag="VN444_02205"
     CDS             complement(1326..2579)
                     /locus_tag="VN444_02205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWR20656.1"
                     /translation="MTAQDTHPTARSAFDVTRIREDFPALRQQIRGKPLVYLDNAATS
                     QKPMRVIETIEKYYLTENSNVHRGIHLLSERATEAFEGARAKVARFLNASDVREIIFV
                     RGATEGINLVAHSFARPLLTVGDEILISEMEHHSNIVPWQIVCKETGAVLRVVPINDD
                     GELRLDEYERLLGPRTRLVSIVHVSNALGTINPVQQMIEMAHERGVPVLVDGAQAAPH
                     LPVDVQALGCDFYVCSGHKLFGPTGIGIVFGKARLLDAMPPYQGGGDMILSVTFEKTI
                     YNEAPLKFEAGTPHIAGAIGLGAAIDYVSSIGLDQIAAYEGELLAYATDAVSAIPGLR
                     IIGTAKEKASILSFVLDGVHAHDIGTILDQEGIAIRTGHHCAQPVLQRFGVPATARAS
                     VAFYNTRGEIDALVKAIHKVTELFG"
     gene            complement(2594..>3114)
                     /locus_tag="VN444_02210"
     CDS             complement(2594..>3114)
                     /locus_tag="VN444_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWR20657.1"
                     /translation="LNVALDGEGAECTLNGLYMVTGRQHVDNHTRIDHAQPHCSSRQL
                     YKGILDGKSKGVFSGKIVVHKAAQKTDAKQTNKNLLLSEDAVIDSKPQLEIFNNDVKC
                     THGTTIGQHNPEAMFYLRSRGVDLVAAGSLLTYAFASEMLGRIRVEPVRAQLEALLLT
                     RLCDGAAPEETP"
BASE COUNT          619 a          981 c          859 g          655 t
ORIGIN      
        1 atataaaggg gggggatttc gtaccccgtt cggagggctg ccacaagagc ttccttagtg
       61 tttcgcgggg tacttcttgt aatagaggtt ggcaacgctc ccctcctcct ccttctgggc
      121 ttcccacaca gctcgctgga ccgccactgg aagggtatgt ccatccttgc acgcatcagc
      181 gttcatctgg atgtactgtt gccactgctc cggcacctgg gtcgcttcga agattgcggc
      241 aaccgggcac tccggctcgc aggcggcgca atcgatgcac tcctccgggt gaatccaaag
      301 gatctcctca ccctcgtaga agcactccac agggcagaca tctacgcacg catggtcttt
      361 ggtaccgata catggctcgg ctacaatgta cgccatcgtt cttctcctcg ctggttaagt
      421 ggtttgggtt gactgaagtt ctgatgcacg cactaagctt ttactacagg aagccaagct
      481 ggagcttagc ggcttcggac agcttggacg aatcccatgg gggatcccag accacctcga
      541 cttgggcatc caccacgcca ggaacgcacc gcaccttcac ctcgactcct gccggcagct
      601 ctgctgcggc tggacaatga ggtgacgtga gggtcatcac caccctggct aagccagaag
      661 gctccacctt cacctcatag atcagcccca gatcgtagat gttgacaggg atctcaggat
      721 cataaacggt gcgcagggcc tcgatgacct ctgcctcgat ttcactcgta ttcataagta
      781 atcagctccc agcgatcagc cgtcagctca tgcaagaacg ctgagtgccg aaggctgatc
      841 gctgccgttg ttggccttga tccgagaaac atcctattcc gtcgaggaga tctgccctct
      901 cccctccagc gcagcctgta aggtatgcca aggcaaagta gcacatttaa cccgaactgg
      961 gaactctcgt acacccgcaa aggcggccaa cttcccaact acgagcggat cgcatgcagc
     1021 gctcagatcg gccgtaacca ttctatgaaa ggtctcgaaa aggccctcgg cttccgctgt
     1081 agtcttgcct ttcacggccg cagtcatcat tgatgcggag gctttcgaaa ttgcgcagcc
     1141 cgatccctga aaggcgatat ccctgatcac atgatcttcg acaagcaggt acaccgtaat
     1201 ctgatcgccg cacagcggat tgtaaccgtc cgccgcacgg ttcgcgcctt ccagcttctg
     1261 gaaatttcgc ggccgcttat tatggtcaag gatgacctcc tggtagagct cacgcaggtc
     1321 cgacatcagc caaacagctc ggtgaccttg tggatcgcct ttaccagtgc gtcgatctcg
     1381 cctcgtgtat tgtaaaaagc caccgacgcc ctggcggtgg ccggcacgcc gaaccgctgc
     1441 agcaccggct gggcacagtg atggcccgtc ctgatcgcaa tcccctcttg atccaggatc
     1501 gtgccgatgt cgtgcgcgtg gacaccgtca aggacgaagg acaggatgct cgccttctct
     1561 ttggcggtcc cgatgatccg taagcccggg atggcggaga cggcgtccgt cgcgtacgcc
     1621 agcaactcgc cctcgtaggc ggcgatctga tccagcccga tgctgctcac gtaatcgatc
     1681 gccgcgccga gcccgatggc gcccgcgata tggggtgtcc cggcctcgaa ctttaacggc
     1741 gcctcgttat agatcgtctt ctcgaaggtg accgagagga tcatgtcgcc cccgccctgg
     1801 tatggaggca tagcatcgag gaggcgcgcc ttaccgaaca caatgccgat gccggtcgga
     1861 ccaaacagct tgtggccgga gcacacatag aagtcgcagc cgagcgcctg cacatccacc
     1921 ggcaaatggg gcgcggcctg ggccccatcg accaggacgg gcacaccccg ctcgtgcgcc
     1981 atctcaatca tctgctgcac cgggttgatc gtaccaaggg catttgagac gtgtacgatc
     2041 gacaccagcc gcgttctcgg gccaagcagc cgctcatact cgtcgagtcg taactcaccg
     2101 tcgtcgttga tcggcacgac ccgcaggacc gcgccagttt ccttacacac gatctgccat
     2161 gggacgatat tcgagtggtg ctccatctcg gagatgagga tctcgtcccc caccgtaaga
     2221 agtggcctgg cgaagctgtg ggccaccagg ttaatcccct ctgtggcgcc gcgaacgaag
     2281 atgatctcgc gcacatcact tgcgttaaga aagcgcgcca ccttggccct ggcaccctca
     2341 aaggcctccg tggccctctc gctcaagagg tggatccccc ggtgtacatt cgagttttca
     2401 gtcaggtaat acttctcgat cgtctcgatg acacgcatcg gcttctgact cgtagccgcg
     2461 ttgtcgaggt acaccagcgg tttgccgcgg atctgctgcc gtagagcagg gaagtcctcc
     2521 ctgattcgcg tcacgtcgaa tgccgagcgc gccgtggggt gcgtatcctg tgcggtcatc
     2581 gccgcgaccg ctctcatggc gtctcctcgg gcgcggcgcc atcgcaaagc cgcgtaagca
     2641 ggagcgcttc gagctgggct cggaccggct caaccctgat ccggccgagc atctcgcttg
     2701 caaacgcata ggtcagcaga ctcccggcag ccacaagatc gacgccgcga gaacgcagat
     2761 aaaacatcgc ctccgggtta tgctggccaa tggtcgtacc atgggtacat ttcacgtcat
     2821 tgttgaagat ctctagctgc ggcttgctgt cgataacagc gtcctcggac agcaggaggt
     2881 tcttgttggt ctgcttggcg tcagttttct gggcagcctt gtgtacgacg atcttgccgc
     2941 taaagacccc cttcgacttc ccatcaagga tacccttata gagctgacgg ctgctgcaat
     3001 gtggctgcgc atgatcgatt cgcgtatggt tatccacgtg ctgccgaccc gtcaccatat
     3061 agaggccatt gagagtgcat tcggcgccct ccccgtcgag cgccacgttc agat
//
LOCUS       DATMXI010000121         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_323465, whole genome shotgun sequence.
ACCESSION   DATMXI010000121 DATMXI010000000
VERSION     DATMXI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323465"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="lon"
                     /locus_tag="VN444_02215"
     CDS             <1..1755
                     /gene="lon"
                     /locus_tag="VN444_02215"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWR20658.1"
                     /translation="KAIQKELGETDDRSQELQVLEKKIQKAKMPAAVESEAKAQLGRL
                     SRMHPDAAEASVIRTYMDWLIELPWSRPTKDKLSIKQASKILNEDHYDLEKVKERILE
                     YLAVRKLKKKMKGPILCFVGPPGVGKTSLGRSIARAMGRKFIRISLGGVRDEAEIRGH
                     RRTYIGALPGRIIQGIKQAGSNNPVFMIDEVDKVGTDFRGDPSSALLEVLDPEQNYGF
                     SDHYLGVAFDLSNVMFICTANTADPIISALRDRLEIIDISGYTEEEKLHIANRYLVPR
                     QYQEHGIDEKRLLITDEAILKMINEYTREAGLRNLEREIATICRKIARLVAEGQKGQT
                     KVTPAVLQRFLGAPKFLADSEQEADEVGVATGLAWTQTGGDIIYIECSILRGKGSLSL
                     TGHLGEVMKESAQAALSYARSRSADLGIKDEFFTKSDIHIHVPAGAIPKDGPSAGITM
                     AVALLSALTKVPVRRDVAMTGEVTLRGKVLPIGGIKEKALAARRMGIHTVVVPARNDK
                     DIKELPANVKRGMEFVFVNHMDQVLEIALVKQTGAKRRLAAALSAKRGATPSTGLRTR
                     LPRHRHPAAPAQGTSYSV"
     gene            1748..>3166
                     /locus_tag="VN444_02220"
     CDS             1748..>3166
                     /locus_tag="VN444_02220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWR20659.1"
                     /translation="MSNDRTVRIVPLGGLGEIGLNMMIVEAAGDLLVIDAGLMFPDDE
                     VLGIDYVIPDMSYLLTHREKVRAVLLTHGHEDHIGALPYLLSKIKAPVYGTRLSLGLA
                     AEKLKEANLLSDADLKPIRPRDRLQFGLFEVEFLQVCHSIPDGVALAIRTPGGLIIHT
                     GDFKFDQSPVDVQLTDYRRLAELGDGGVLALLSDSTNAGRDGFTPSEQVVGQAFDAIF
                     RETQGRVIVACFASNIHRVQQILDAAAATGKRVAICGKSMVANVRIATELGCLRTPDA
                     TLVSLDELERLPIAQRVIVTTGSQGEPLSAIARMAVGAHKQVQVSPGDTVIFSSRVIP
                     GNERSVARTIDGLYRQGARVITEEVAGVHVSGHASREELKLMLNLTRPTFFVPIHGEY
                     RHLHLHAHLAREVGVPEARTLVIEDGDILEFDDSSARIVGRAPVGRIFIDGKGVGNVG
                     DAVIRDRQRLAQEGVVVVVLAVD"
BASE COUNT          654 a          883 c         1028 g          601 t
ORIGIN      
        1 aaggccatcc agaaagagct gggtgagaca gatgaccgaa gccaggagct gcaggtgttg
       61 gagaagaaga tccagaaggc taagatgccc gcagcggtcg agtctgaggc gaaggcgcag
      121 ctagggcggc tcagccgcat gcaccccgac gcggcagagg cctctgtcat ccggacctat
      181 atggactggt tgatcgagct gccttggagt cggccgacca aggacaagct atcgatcaag
      241 caggcatcga agatcctcaa cgaggaccac tacgacctcg aaaaggtgaa ggagcgaatc
      301 ctcgaatatc tggctgttcg caagctgaag aagaagatga aggggccgat tctctgcttc
      361 gtcggcccgc ctggggtggg gaagacctcc cttggccgtt ccattgcccg cgccatgggg
      421 cgcaaattca tccggatctc gttgggcggc gttcgtgacg aggcggagat tcgcggtcat
      481 cggcggacgt atatcggggc gctacccggt cgtatcatcc aggggatcaa gcaggccgga
      541 tcgaacaatc ccgtcttcat gatcgacgag gtggacaagg tcgggaccga ttttcgtggc
      601 gacccttcct cggccctgct ggaggtgctg gacccggagc agaactatgg cttcagcgac
      661 cactacctcg gtgttgcttt tgatctctct aacgtgatgt tcatctgtac ggccaacacg
      721 gctgacccga tcatctcggc gctgcgcgat cggctggaga tcatcgatat ctccgggtac
      781 accgaggagg agaaactcca tatcgccaat cgctatctgg ttcccagaca gtaccaggag
      841 catgggatcg acgagaagcg cctgctgatc accgacgaag cgatcctgaa gatgatcaac
      901 gagtataccc gggaggccgg gctccggaac ctggagcgag aaatcgccac tatctgcagg
      961 aagattgccc gcctggtggc ggaagggcaa aaggggcaga ccaaggtgac cccggcggtc
     1021 ctgcagcggt ttctgggcgc accaaagttt ttggccgact cggaacagga ggccgatgag
     1081 gtcggtgtcg ccacgggtct cgcgtggacg cagactggcg gggacatcat ctacatcgag
     1141 tgcagcatcc tgcgcgggaa gggcagcctc tcccttacgg ggcatctggg tgaggtgatg
     1201 aaagagtcgg cccaagccgc cttgagctat gcacgctcgc ggtccgccga tctggggatc
     1261 aaggatgaat ttttcaccaa atctgatatc catatccacg tcccggccgg agcgatcccc
     1321 aaggatggtc cgtcggccgg catcacaatg gccgtggcgc tgctgtccgc cctgaccaag
     1381 gtgcccgtga ggagggacgt ggcgatgacc ggcgaggtga ctcttcgcgg caaggtcctg
     1441 ccgatcggcg gaatcaagga aaaggcattg gcggcgagaa gaatgggaat ccatacagtg
     1501 gtggtcccgg cgaggaacga caaggacatc aaagagctcc ctgccaatgt gaagcgtggt
     1561 atggagttcg tattcgtgaa ccatatggac caagtgctcg aaatcgccct ggtcaagcag
     1621 acaggggcca agcgccgcct cgctgcggct ctcagcgcca agcgtggggc aaccccttcg
     1681 accgggctca gaacgcgctt gccgaggcac cggcaccccg cggcccctgc gcaaggaact
     1741 tcatacagtg tctaacgacc ggacggttcg gatcgtcccc ttgggaggtt tgggggagat
     1801 cggattgaac atgatgatcg tcgaagcggc gggcgatctg ctggtgatcg atgccgggtt
     1861 gatgttcccg gatgatgagg tgctcgggat cgactacgtg attccggata tgagctatct
     1921 gcttacgcat cgagagaagg tgcgggcagt gctgctcact cacggccacg aggatcatat
     1981 cggcgcgctt ccttacctgc tgagcaaaat caaggctcca gtttatggta cgcgcctctc
     2041 gctggggctg gcggcggaaa aactgaagga agcgaatctc ctgtccgatg ccgacctcaa
     2101 gcccattcgt ccgcgagatc gccttcagtt cggcctcttc gaggttgagt ttcttcaggt
     2161 ctgccacagc attccagacg gcgtggcact ggccatcagg acaccaggtg gcctgattat
     2221 acacaccggc gatttcaaat ttgatcagag cccggtggat gtccaactga ccgattatcg
     2281 gcggcttgcc gagctcgggg atggtggcgt gctggccctt ctctcggata gtacgaatgc
     2341 gggccgggat ggatttacgc cttcggagca ggtggtaggg caggctttcg acgcaatctt
     2401 tcgagagact caggggcgcg tgatcgtggc ctgtttcgcc tcaaacatcc atcgcgtcca
     2461 acagattctc gatgcggcgg cagctacggg aaagcgggtg gcgatttgtg gcaagagcat
     2521 ggtggccaac gtgcggatcg cgacggagtt gggatgcctc cggacccctg atgccacgct
     2581 cgttagtctg gacgagttag agcgcctccc gattgcgcaa cgggtgatcg tgaccactgg
     2641 gagccaaggc gaaccactat ccgctatcgc cagaatggct gtcggggcgc acaaacaggt
     2701 ccaagtgtcg cccggcgata ccgtcatctt ttcatcgcgc gtcattcctg gcaatgaaag
     2761 atcggtggct cgaacaattg acggcctcta ccgacagggg gcccgtgtca tcactgagga
     2821 ggtagccggg gtccatgtct cgggacatgc cagccgggaa gagctgaagc tgatgctgaa
     2881 tctgacccgt cccaccttct ttgtcccgat tcacggagaa taccgccacc tgcacctgca
     2941 cgctcacctg gcacgagagg tcggggtgcc ggaagcccgg accctcgtca tcgaggacgg
     3001 cgacatcctg gagttcgatg acagcagcgc ccggatcgtc ggtagggcgc ccgtcggtcg
     3061 gatcttcatc gacgggaagg gggtcggaaa tgttggggac gccgtcattc gcgaccggca
     3121 gcggttggcg caggaggggg tcgtggtcgt ggtccttgca gtagat
//
LOCUS       DATMXI010000122         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_38906, whole genome shotgun sequence.
ACCESSION   DATMXI010000122 DATMXI010000000
VERSION     DATMXI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38906"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VN444_02225"
     CDS             complement(<1..963)
                     /locus_tag="VN444_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HWR20660.1"
                     /translation="MTRFWKSGDPFIWLTGGALALSLLMVAGLVLLVLLNGLGFFWPS
                     PIVHLTLSDGKELLGQVMQQEVIPQPDAPPGTATRYRIQVKVGNRDLYGADFVWVEEA
                     TIARRDFPAEAVLIERREWGNLYGFLKEVRGGDNERAVVQGPEPAWSALQALLPDATS
                     TFKEIRRIEKKEIGAINSAQEKIRLKLKKLQLSGQEGGSEAARLNKEIEGWAAKYREQ
                     EAKLATVRQLSRQTLIVSVIGDQETELPLQQIVRIVRPNSMGVWSKSGVYLSRLWEFI
                     SGDPRESNTEGGVFPAIFGTVMMVMIMSLLVAPLGVLAAFYLREY"
     gene            complement(960..3230)
                     /locus_tag="VN444_02230"
     CDS             complement(960..3230)
                     /locus_tag="VN444_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWR20661.1"
                     /translation="MEDSTKVAVPGEPLQVARPVIVPREKIARRLLLDRLARWVVTLG
                     GAAIIVSILAILFVIAAEVYPLFRKPTAVLLGALTTSLDSAPLAVGVDEYREIVYVVT
                     ASGVHFVSVKDGTLLTSNRLQELHGATVVGTSALGRGQFALGLSDGRAIPAEVGFSVT
                     FPEGKRRIEAEFVTGDPITVDPERHPLARLAYASTPNGPMTAAAVGPQALILVTVKET
                     TALIGPSTKEESRERLTLPVEGEITQLLLDGRGENLFAGTSSGQVIRVDLQNPSDPTV
                     AGVVPATIRPGIGVSAMGFLIGDRTLVVGDTMGGVSSWQLVQADGEGQRLAKVHDFLA
                     HTGPVVAFAPSRRDKGFVTADASGIIHIHYGTTGKTLLTLKAADVGLSAATFAPKVDG
                     VMTVDAKGGLSHWTVENPHPEISWGSLFGKIWYEGYPAPAYVWQSTGGTDDFEAKFSL
                     APLIFGTIKGTFYALLIAIPLALLGALYTSQFMHPTLRGIVKPTVEIMAALPSVVLGF
                     LAGLWLAPLVEQVVPGLFLMPIVTTLLILIVVFCWRFVPIGVRSRVKAGTEVALLIPI
                     VILGGWISFQLGGAVERLLLSGDYRGWLLNVLGLTYDQRNCLVVGIAMGFAIIPIIFT
                     IAEDSLSNVPQHLVAGSLALGATRWQTALRVVLPTASPGIFSAIMIGFGRAVGETMIV
                     LMATGNTPVMDWSIFNGFRALSANIAVELPEAPDGGTLFRVLFLAALLLFVMTFLVNT
                     LAELVRLKLRQRYRAL"
     gene            complement(3250..>3592)
                     /locus_tag="VN444_02235"
     CDS             complement(3250..>3592)
                     /locus_tag="VN444_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HWR20662.1"
                     /translation="GSASVVQGVAVDRYAMGYSGIGYTTAGVRAVPLAEKEGGACGEA
                     TAANAYSGKYPLSRFLFIYVNRAPGKRLDPLTSEFIGLVLSKEGQAVVIKDGFFPIPN
                     SVANEELSKAR"
BASE COUNT          710 a         1122 c         1136 g          624 t
ORIGIN      
        1 gtactcccga aggtagaacg cggccagtac gcccagcggc gcgacgagga ggctcataat
       61 catcaccatc atcaccgtgc cgaatatggc ggggaagacg ccaccctcgg tgttcgattc
      121 cctgggatcg ccggagatga actcccagag cctggacaga tacacgccgc tcttggacca
      181 cacccccatc gagttcggtc ggacgattcg gacgatctgc tgaagcggta gctccgtctc
      241 ctggtcgccg atgaccgaga cgatgagcgt ctgtcgggag agctgacgga ccgtggcgag
      301 ctttgcctcc tgttcgcggt acttggccgc ccaaccctca atctccttgt tgagccgtgc
      361 tgcttctgaa cccccctctt gccccgagag ctggagcttt ttgagcttga ggcggatctt
      421 ctcttgggcg gagttgatcg ccccgatctc ttttttctcg atccgcctga tctccttgaa
      481 ggtcgatgtc gcgtccggga ggagagcctg aagagcgctc catgccggct caggtccctg
      541 aactacggcg cgctcgttgt cccccccacg aacctccttg agaaagccat agagattgcc
      601 ccattcccgt cgctcgatga ggacagcctc cgcagggaag tcgcggcgag cgatcgtggc
      661 ctcctcgacc cagacgaagt cggcgccgta caggtcacga ttgccgacct taacctgaat
      721 ccggtagcgc gtggcggtcc ctgggggtgc gtccggttgc gggattacct cctgttgcat
      781 cacctggcct aatagctcct taccgtccga cagcgtcaga tgcacaatcg gcgacggcca
      841 aaagaagccc agcccgttga gcagcaccag gaggacaagc ccggccacca tgagaaggct
      901 gagggctaat gcccctccag tgagccagat gaacggatcg ccgctcttcc agaatcgagt
      961 catagggcgc gatacctctg ccggagcttc aggcggacca gttcggccag ggtgttcacg
     1021 aggaatgtca tgacaaagag caggagggct gccaggaaga gcacccggaa gagcgtccca
     1081 ccatcaggcg cttcggggag ctccacggcg atgttggccg agagggcccg aaagccgttg
     1141 aagatgctcc agtccatcac cggcgtattc cctgtggcca tcaggacgat catcgtctcg
     1201 cccaccgccc gtccgaagcc gatcataata gcggagaaga tccccgggct cgccgtcggc
     1261 aggacgaccc tcagcgcggt ctgccaccgg gtggcgccaa gcgcgagcga accggccaca
     1321 aggtgttgag gcacgttgga gagcgaatcc tcggcaatgg taaaaatgat cgggatgatg
     1381 gcgaagccca tggcgatgcc cacaaccagg cagtttcgtt gatcataggt gagcccgagg
     1441 acattcaaga gccagcctcg atagtcgcct gacagcaaca ggcgctcgac cgccccaccg
     1501 agttggaagg agatccagcc tccgaggatc acgatcggga tgagcaaggc gacctcggtg
     1561 cccgccttga ctcgactgcg gacgccaatg ggaacgaatc gccagcaaaa gacgacgatc
     1621 aggatgagga gtgtggtcac gatcggcatg aggaagaggc cgggaacgac ctgttcgacg
     1681 aggggcgcaa gccaaaggcc tgccaggaag cccaggacga cgctcggaag cgcagccata
     1741 atctccacgg tcggcttcac gatccccctg agcgtcgggt gcatgaactg ggaggtgtac
     1801 agggcgccca ggagcgccag cgggatcgcg atgagcaggg cgtagaatgt ccctttgatc
     1861 gtcccgaaga tgagcggggc gaggctgaac ttcgcctcaa agtcgtcggt ccctccagtg
     1921 gactgccaga cgtatgccgg cgcggggtac ccttcgtacc agatcttccc gaacaggctc
     1981 ccccaactga tctcggggtg ggggttctcc acggtccagt gtgagaggcc ccccttcgca
     2041 tcaactgtca tgacgccatc gactttgggg gcgaaggtgg cggcgctgag tcccacatct
     2101 gcagccttga gcgtcagcaa ggtcttgccc gtggttccat agtggatgtg gatgatgccg
     2161 gaggcgtccg ccgtcacaaa ccccttatcg cggcgggacg gggcgaaggc gacgacgggc
     2221 cccgtgtggg ccagaaagtc atggactttc gccaggcgct gcccttcgcc atccgcctgc
     2281 acaagctgcc aggagctgac gccgcccatc gtatcaccca ccaccagggt gcggtccccg
     2341 atcaggaacc ccatcgcgct gacgccgatc cccggtcgga tcgtggcagg gacaacgccg
     2401 gcaaccgtcg ggtcgctcgg attttgcaga tcgaccctga tgacctggcc ggacgaggta
     2461 ccggcgaaca ggttctcgcc gcggccatcg aggaggagct gagtgatctc cccctcgacc
     2521 ggcagagtga ggcgctcacg tgactcctcc ttcgtggatg gaccgatcag ggccgtcgtc
     2581 tctttgacgg tgacgaggat gagagcttgt ggaccgacgg cagccgcggt catgggcccg
     2641 ttgggggtgg aggcgtaggc gagccgggcg agcggatgcc gctccggatc gaccgtgatc
     2701 gggtcgccgg tcacgaattc agcttcgatg cggcgctttc cttccgggaa ggtgaccgag
     2761 aatccgacct cagcggggat cgcacgccca tccgagagtc ccagtgcgaa ctggcctcgt
     2821 cccaaggccg atgtcccgac gaccgtggcg ccgtgtagtt cctgcagccg gttggatgtc
     2881 agtaaggttc cgtccttgac ggaaacgaag tgtacgccgg aggccgtcac cacgtatacg
     2941 atctcacgat attcatccac ccctactgcc agcggggccg aatcgaggct cgtggtgagc
     3001 gctcccagca gtaccgctgt gggctttctg aagaggggat agacctccgc ggcaataacg
     3061 aacaggatgg ccagaataga gacaatgatc gccgcccctc cgagcgtgac gacccaacgg
     3121 gcgaggcgat ccagcaggag gcgccgcgca atcttttcgc gcgggacgat aaccgggcga
     3181 gccacctgca gcggctcgcc cggtacggca accttcgtgc tatcttccat ggagcggctt
     3241 agtaggccat tagcgtgctt tgctcagctc ttcgttagca acggagttcg ggatcgggaa
     3301 aaatccgtcc ttgatcacga ccgcctgccc ctccttcgag agcacaagcc caatgaactc
     3361 actggtgagg gggtcaaggc gtttgcctgg cgcccgattg acgtagatga acagaaaccg
     3421 tgacagtggg tactttccgg aataggcgtt ggccgcagtg gcctcgccgc acgcgccgcc
     3481 ttctttctcg gccagtggaa cggcccgcac gccggcggtg gtatagccga tgccactata
     3541 gcccatggcg tagcgatcaa cagccacccc ctggacgaca gaggccgagc cg
//
LOCUS       DATMXI010000123         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_297258, whole genome shotgun sequence.
ACCESSION   DATMXI010000123 DATMXI010000000
VERSION     DATMXI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297258"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VN444_02240"
     CDS             <1..781
                     /locus_tag="VN444_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWR20663.1"
                     /translation="CGRCRYCRHGSESMCPQFKATNIDPGGFAEFVRIPALQIEQTAF
                     ALPEELPDDAGIFMEPLACAVRAVKRSDVKAGDQVVVVGAGCMGLLVAQTIAAFGARP
                     ICVDLRDERLELARMLGVEVTLNVARSDPRMALRSVTEEDGIDGAILTAVSAPMLEAV
                     LGVLRAGGKINIFADAGDAGRMAIDLSAFYRQELSLMATYSATPVELGEAIQLLASGQ
                     IQTFPLISHRFDLSRFDEGARLQREGQATKVLFLPSVVSTS"
     gene            778..1857
                     /locus_tag="VN444_02245"
     CDS             778..1857
                     /locus_tag="VN444_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWR20664.1"
                     /translation="MIPVNVLSRTMVAAVLHGAKDVRLEEVPIPRLGPEDLLARVEAA
                     SIDFTDRKVYLRGSHPMIEIPGLFGHEWAGIVVARGEQADARWQPGMRVVAANSAPCT
                     DPVPAARCRACRRSRQGMCERLLYNNGAFAPYIRIPGRIAEVNLYELPPQIPFEEAAL
                     AEPLACVMHAIRRVQVSDGDRVVVVGAGPIGLMFISALRNRYGQAIRLLSLDHHDDRL
                     ALARSFGADEALNTAGGPLMAGVREAFGAERADVVIEAVGSTQAHQEAFELVGRGGTL
                     VPFGGVAEGDLLSLDLHRLHYEEIRIVPIYHHTPGDFASAVHAIVRREVPVARLITAR
                     LPLMELPRALEMVQERTTLRTILFS"
BASE COUNT          337 a          578 c          695 g          421 t
ORIGIN      
        1 gtgtggccgg tgccgctact gtcgccatgg cagcgagtcg atgtgtcccc agttcaaggc
       61 gaccaatata gatcccggcg gcttcgctga gttcgtccgg attccagccc tgcagataga
      121 gcagacagcc tttgcgcttc ctgaggagct tcctgacgac gcaggtatct tcatggagcc
      181 attggcgtgc gctgtgcggg cggtcaagcg atccgatgtc aaggctggtg atcaggtcgt
      241 ggtagtcggc gccgggtgca tggggctgct ggtcgcgcaa accatcgctg cttttggcgc
      301 gaggccgatc tgtgtggatc ttcgggatga gcgtcttgag ttggctcgaa tgctcggcgt
      361 tgaagtgacc ctgaatgtgg ccagatcgga tccacgaatg gctcttcgct cggtcacgga
      421 agaggacggg atcgacgggg ctatcctcac tgcggtcagc gccccgatgt tggaagcggt
      481 gctcggcgtc ctgcgtgccg gggggaagat caatattttc gcggatgcgg gcgacgccgg
      541 acgcatggcg attgatctga gcgccttcta ccggcaggag ctgtccctca tggcgaccta
      601 ttcggccacg ccggtggaac ttggcgaggc gatccaactc ttggcctccg ggcaaatcca
      661 gacttttccg cttatcagcc atcgcttcga cctgagccga ttcgatgagg gggcgcgtct
      721 acagcgggaa gggcaggcca cgaaggtcct tttcttaccg tccgtggtaa gcacgtcgtg
      781 atccctgtga atgtcctgag ccggacgatg gtggcggcgg ttcttcatgg cgcgaaagat
      841 gtgcgcttag aagaggtgcc gattccgcgg cttggcccgg aggatctcct ggctagggtc
      901 gaggccgctt ccatcgattt cacggatcgc aaggtgtacc tgcgtggatc gcacccgatg
      961 attgagatcc ccgggctctt cgggcacgag tgggccggga tcgtggtggc gcgaggggag
     1021 caggctgatg ccaggtggca gccggggatg cgggtggtcg cagccaactc ggccccctgt
     1081 accgatccgg ttccggcggc ccgctgtcgt gcgtgccggc gttctcgtca gggtatgtgc
     1141 gagcgactgc tgtacaataa cggcgccttt gccccctaca ttcgcatccc tggacggatc
     1201 gcggaggtga acctgtatga gctgcccccg cagatcccct ttgaggaggc agctctggcc
     1261 gagccgctcg cgtgcgtcat gcacgccatc cggcgcgtgc aggtgtccga tggagatcgt
     1321 gtcgtggtcg tgggagccgg gccgatcggc ctgatgttta tatccgccct ccgtaaccgg
     1381 tacgggcagg ccattcgtct cctcagcttg gaccatcacg atgaccgtct ggccctggca
     1441 aggagcttcg gtgcggacga ggcgctaaac actgccggag ggccgctgat ggcaggagtg
     1501 agagaggcct tcggggcaga gagggctgat gttgtcattg aggcggtggg aagtacgcag
     1561 gcgcaccagg aagcatttga gctagtgggc cgggggggca cgctggtccc cttcggagga
     1621 gtggcggagg gcgatctcct ttccctcgac ctgcatcggt tgcattacga ggagattagg
     1681 attgtcccga tctaccacca tacccctggc gatttcgcca gcgcggtcca cgccatcgtt
     1741 cgccgcgagg ttccagtggc ccgtctcatc actgcccgcc tccccctcat ggagttgccg
     1801 agagcactcg agatggtgca agagcggacc acccttcgga cgatcctgtt ttcatgaggt
     1861 cggcttagca tttgagaagg tccatgatga agaagctggg gaatgggact caacggatga
     1921 ggacgatccc gctggcctcg ttgtcgggcg ttacgcgcga aagacggtca gtcgtgccgg
     1981 ctcgtgtgga ccagcatgcg ccggttcaag gtcgaggcga ctctacttca c
//
LOCUS       DATMXI010000124         7116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_126640, whole genome shotgun sequence.
ACCESSION   DATMXI010000124 DATMXI010000000
VERSION     DATMXI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126640"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1165
                     /locus_tag="VN444_02250"
     CDS             326..1165
                     /locus_tag="VN444_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR20665.1"
                     /translation="MAIGDLTRINTNIAAFNALNSLRTIGNQLSTAQLRLQTGKRINE
                     AADDPAGFSIVSKLTSRARGLATALDSVGTSTNLLSIAEGSAQSIHDNLLNIQDLIRQ
                     ATSDNLGTSERNAIEQQIDDLSAEITRLRDATTFNGVKLLDGTFTGKRILTGSESTET
                     ILASISQDFSLASLGITDASIAVDTATNASLSLTYVDAALTSVRLEIQNVGALGSRLR
                     ATQDNLAVAITNTRAAASRVQDADVAAEQVNAVRLQILQQLATAQLAQANSGPQQVLA
                     LFR"
     gene            1277..1696
                     /locus_tag="VN444_02255"
     CDS             1277..1696
                     /locus_tag="VN444_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HWR20666.1"
                     /translation="MPSHVGADSVAVRSDSLRAAPLTGSPMAAYQSAQVLGASPMQLI
                     LIVYDVALAACGRRDAVRARRAITELIAALNFDYEEIAVPLFRLYEYCLNAIGSGSFH
                     EASKILRQLKETWETASRQAPVEQNLKSDVRSVRRKE"
     gene            1700..3163
                     /gene="fliD"
                     /locus_tag="VN444_02260"
     CDS             1700..3163
                     /gene="fliD"
                     /locus_tag="VN444_02260"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HWR20667.1"
                     /translation="MAITEILSASAFQLNIEALVSSVMTVERRPLAGLQSDQMALSRR
                     SATLSDLKSSLSALRSKAQAVAQVGTLSPFQVKAVSSSNMSVATATASSSALAAVHAL
                     SVSQLAKSSTVVSKQLTSTGTDVVTVEGAGTKSVKVTCDGTDPATSGTAVTVNVTLNA
                     GDTDSTILTNLAAAINNDATLGAKVSASVVNDTSDTSRLVLTGKQTGLANKVRMADAA
                     GTLLNAIELNSEVASSGTAGGYLFADATLDAKFKLDGLDITRSSNSVSDVLTGVTINL
                     LGLSATDVTLTVSPDKSAMKTSVQEFLDAYNKALTFLRGRIAVTVSATTSGTNATEVN
                     SVIRGPLAGEPTYTALVSTLRSEVGSSVSGVQTGNPALLAEVGITAAANGTLSISDTT
                     KFDSAIATKLSGLIDLFASGSGVSTRLIARLDGFVNSGALIDGSLSTVTSKTNNINQQ
                     ITRLQERLDRREAALRKQLLDLQKALSALSAQRFILE"
     gene            3192..3557
                     /locus_tag="VN444_02265"
     CDS             3192..3557
                     /locus_tag="VN444_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HWR20668.1"
                     /translation="MADIGHESKPIEPMMGVRSSGGEISAALPPERGSHNAAGSGASS
                     LARLEVAMPDLKKVLENLSPHQNVGLAYVIDRESHSVIIKVIDRDTNEVIRQVPSEEM
                     TKLRAVMRGLFGLLFKAEA"
     gene            3598..3933
                     /locus_tag="VN444_02270"
     CDS             3598..3933
                     /locus_tag="VN444_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20669.1"
                     /translation="MNAEETTRQYDEILELTLQQREAIGSGDLERLLTLLARRETLLA
                     SLAAQNGVRYQAWRRQIAELDSANEAALLTWREQATVELASIRRGRTGLGGYRACAPV
                     EDTFIDRIS"
     gene            3945..4292
                     /locus_tag="VN444_02275"
     CDS             3945..4292
                     /locus_tag="VN444_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20670.1"
                     /translation="MRIDQIRILEVVRAYEKSRAGVGRRQEQDADTYPAVRDEVIIST
                     EAKRRQILDRVLGQVVERLKSLPPSEIRLPDIDAVLEEAAQDIGRAPLDPEEKAHLRE
                     TGLADLRRARVLR"
     gene            4292..4972
                     /locus_tag="VN444_02280"
     CDS             4292..4972
                     /locus_tag="VN444_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20671.1"
                     /translation="MERSVHSSTTEADVTAGPYSLLIVDDDELVLSVLYDLLAREAGH
                     RISTAADPRTALELLRNEPFDLLITDYRMPEITGLDLIREATRLHPDLIGILITGFVW
                     PDAAAEATEAGAYDLMLKPLNIGEVCVRVRNACERIRLVRENRRCQRELEQAMRMTTR
                     DAQRMTPSEAIAEIGLFPPNLSSAPKGTRKEEQMLNQLERLGKLYQMGLLTKEEFVLS
                     KSKLFGRT"
     gene            4992..6464
                     /locus_tag="VN444_02285"
     CDS             4992..6464
                     /locus_tag="VN444_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWR20672.1"
                     /translation="MAEELRVLVAEDNPDILELLGELLEAERCRVTLCSDGASALAQI
                     AAEPFHLILSDITMPGASGLEILDRSSTMAAGIPVVLISGNVDLEAALFALRRGAFDC
                     LLKPFRLDDIRDLVRRVQRLRGLTENGAGELVARASRPNGHRPQEILIRKSRALRLIA
                     QSGRVLGGTHDLGRLMATILELAMRGTGAELGALLVMSEAEERLRVTASKGFGDTVKP
                     GDEVESIDELWIVAGLPPGSASTVVPLIVKGRHVGGMALASRHRSGSFLVADCEMLDI
                     LAQQASMAIENVSLYTAVESSVFEGMRALVATLEAKDPYTEGHSLRLAKAAVAICRQM
                     ELGSHVENLVRYAGALHDIGKVGIPDAILRKPGRLTPEEYERMKEHPVLSWKILLPFS
                     FLQEEAIIVRHHHEWINGNGYPDGLSGSALPLASRILAAADAYDAMTTTRPYRTPRAP
                     EQAIEELRRCAGFQFDSDVVEALAALSPCPYHLDPSDLSR"
     gene            6770..>7116
                     /locus_tag="VN444_02290"
     CDS             6770..>7116
                     /locus_tag="VN444_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20673.1"
                     /translation="MDRVAVLGEQRSTIDLVVTAFEHQGVEVSRIEHQSDVAATLANK
                     PYLLVIFCLEKMESTDLNGLSRLVAASADTPIVPAVFTLSLEMALAAIRLGAFDVLVL
                     PSTTEAVRNLLVRA"
BASE COUNT         1523 a         2020 c         2205 g         1368 t
ORIGIN      
        1 ggagcggtgg gagtcgcgaa atcctcctac ccccccttta caaaaggggg gcacgggagg
       61 atttggcgtg cgaggtgcga ggtacgaagg acgtcgacac ccgacaccag acagctcgca
      121 ccaggcgcag caagcagcgc ccctacacac cctacatcct acatcctata ccctacatca
      181 tatggctgag gatggccgcg ctccgccccc tgaaccgtga accccgaact gtgaaccgtg
      241 aacgcttttt tgggtcgggc aaggatgtcc gacctatata ccaggcaacg tgaagtcgtg
      301 ttgccgctac acggaggtag ccacaatggc cattggtgat ctgacccgta tcaacacgaa
      361 cattgcagcc ttcaatgcat tgaacagcct caggaccatc ggcaatcaac tgagcaccgc
      421 ccagcttcgg ctccagaccg gtaagcggat taacgaagcg gccgacgatc cggccggctt
      481 ctcgatcgtc agcaaactga cttcgcgcgc ccggggctta gccaccgccc tcgattccgt
      541 cggcacatcg accaacctgc tctccatcgc tgagggatct gcgcagtcga tccatgacaa
      601 cctcttgaac atccaggacc tgattcgaca ggctacctcg gataatctcg ggaccagcga
      661 gcgcaacgcc atcgagcagc agatcgacga tctgtcggct gagattaccc gtctgcgcga
      721 cgcgaccacc tttaatgggg tcaagctgct tgatgggacc ttcaccggga aacggatcct
      781 gaccggaagc gagtcaaccg agacaatcct cgcgagcatc agccaggact tctcgctggc
      841 gagccttggc attacggacg ccagcattgc tgtcgatacc gcaaccaacg catccctgtc
      901 gctgacctac gtagacgccg cgctcacgtc ggtcaggttg gagatccaga acgtcggagc
      961 gttgggcagc cggttgcggg ccactcagga taacctcgca gtcgcgatca ccaacaccag
     1021 ggcggcggca tcaagggttc aggatgccga cgtggccgcc gaacaggtca acgccgtgag
     1081 actgcagatc ttgcagcagt tagcgacagc ccagcttgcc caggcgaata gcgggccaca
     1141 gcaggtcctg gcgctcttca ggtaagtacg atagccatca gccatcagcc atcagcagct
     1201 tgtagccccc tcccctccga ggaggggagg gggtgaggct gaagtggtgg ctgaacaccg
     1261 attctaggag gagtaaatgc catcacatgt cggtgctgat tccgtggcag ttcgatctga
     1321 ttcattgcgg gcggcccccc tcaccgggag cccgatggca gcgtaccaga gcgcccaggt
     1381 tctcggggct tccccgatgc aactcattct catcgtatac gacgtggcgc tcgctgcctg
     1441 cggccgccgc gatgccgtac gtgcgcgacg cgccatcacc gagctgattg cggccctgaa
     1501 ctttgactat gaggagattg cagttccctt gtttcgcctg tacgaatact gcctcaatgc
     1561 gatcggttcg gggtcattcc atgaggcgtc aaagatcttg aggcaattga aagagacgtg
     1621 ggagacggcg tcacgccaag ctccggtcga acagaatctg aagtctgacg tacggagtgt
     1681 aagacgtaag gagtagacta tggcgatcac ggagatcctg tcggcgtcgg ctttccaact
     1741 caacatcgaa gcgctcgtat cgagtgtcat gaccgttgag cggcgaccgc tggccggatt
     1801 acagtccgat cagatggcgc tctcacgccg gtctgcgacg ctcagcgacc taaagagttc
     1861 gctgagtgcg ctgcgatcca aggctcaggc cgtggcccag gtgggaacgc tgtcgccctt
     1921 tcaagtcaag gcggtcagct cgagcaatat gtccgtcgcg acggcaaccg cctcatcttc
     1981 cgccctggcc gcagttcatg ccctttctgt ctcgcaactt gccaagtcga gcacggtcgt
     2041 ttcaaagcag cttacctcta cggggaccga tgtggtgacg gtggaggggg ccggcacgaa
     2101 gagcgtgaag gtgacctgtg acggcaccga tccggcgacg agcggtacgg ctgtcacggt
     2161 aaatgtcaca ttgaacgccg gggacaccga tagcacgatc ctcaccaacc tcgctgcagc
     2221 aatcaacaat gacgcgacac tcggcgccaa agtcagtgcc tcagtagtca acgacacctc
     2281 cgacacgagt cgactggtcc tgaccggtaa acaaaccggc ctggccaaca aggttcggat
     2341 ggccgatgca gccggcaccc tgctcaatgc gatcgagctc aacagtgagg tcgcctcgtc
     2401 ggggacggca ggcggctatc tctttgccga tgcaacgctt gatgccaagt tcaaactgga
     2461 tggcctggat attacgcgga gcagcaacag cgtcagcgac gttctgacag gggtgacgat
     2521 caacctgctc ggactctcag ccactgatgt gacgctcacg gtcagcccgg acaagagcgc
     2581 tatgaagacg agcgttcagg agtttctgga tgcgtacaac aaggcgttga ccttcttacg
     2641 ggggcggatc gcagtcacgg tcagcgcaac cacctccggg accaacgcca cagaggtcaa
     2701 ctcggtgatc aggggtccat tggcaggcga gccgacgtat acggcgttgg tgtcgaccct
     2761 cagatccgag gttggcagct cggtgagcgg tgtacagaca ggcaatccgg ccctcctggc
     2821 cgaagtcggc attacggcgg ctgcgaacgg aaccctttct atcagcgata ccaccaagtt
     2881 cgacagcgcg atcgcgacga aactcagtgg gctgatcgat ctctttgctt ctgggagtgg
     2941 cgtctccacg cggctgatcg cccgcctgga tggatttgtc aatagcgggg cactgatcga
     3001 cggcagcctc tctaccgtga catcgaagac gaacaatatc aaccagcaga tcacccggct
     3061 gcaagaacga ctggacaggc gagaagcggc cttgcgcaaa caactgttgg atctgcaaaa
     3121 ggcgctgagc gcactgagcg ctcaacgatt tattctagag taggaggccc ctcagcagag
     3181 gggggaaggt tatggcagac ataggtcacg agagcaagcc gatcgagccg atgatggggg
     3241 tcagatcatc cggaggcgag atcagcgcag ctttaccgcc ggagaggggg agccacaatg
     3301 cggccggatc aggagcgtct tctctcgcaa ggctggaggt cgccatgccc gatctgaaaa
     3361 aggtcttaga gaacctcagc ccgcaccaga atgtcgggct cgcctacgtc atcgaccggg
     3421 agagccacag cgttattatc aaggtgatcg accgcgatac gaacgaggtg ataaggcagg
     3481 tcccttcgga agagatgacg aaactgagag ccgtgatgag gggtctcttc ggattactgt
     3541 tcaaggccga ggcatagggc gttgcgacac ctgcggtgtg tgaaggggca gagataagtg
     3601 aacgctgaag agacgacgcg acagtatgac gagatccttg agctgacgct gcaacagcgt
     3661 gaggccatcg ggagcggcga tctggagcgg ctgctcacgc tgctcgctcg tcgggagact
     3721 cttctggcgt ccctggccgc gcagaacgga gtaaggtatc aggcgtggcg gcggcagatt
     3781 gcggagttgg acagcgcaaa tgaggcggcc ctgcttacct ggcgcgagca ggcgacagtt
     3841 gagcttgcct caatccggcg cggacggacc ggacttggag gctatcgagc ctgcgctccg
     3901 gtcgaggata ccttcatcga tcggatctcc tgaaggacac ggctgtgcga atcgatcaga
     3961 tccggatact cgaggtggtg agggcgtacg agaagagccg tgcaggcgtc ggcaggcgcc
     4021 aggagcagga tgctgatacc tacccggccg ttcgcgacga ggtcatcatc tccaccgagg
     4081 cgaagcgacg gcagatcctg gatcgagtct tgggacaggt ggtcgagcgt ctgaagagtc
     4141 ttccgccatc ggagatccgc cttccggaca tcgacgctgt cctcgaggag gcggctcagg
     4201 atatcgggcg agcgccgctg gacccggagg agaaggcgca tctccgtgag acgggccttg
     4261 ccgacctccg cagggcgagg gtcttgcggt gatggagcgg tctgttcaca gttccacgac
     4321 agaggcagac gtgacggctg ggccgtactc gctgctcatc gtcgatgatg atgaactggt
     4381 cctgtcggtc ctatacgatc ttctggcgcg tgaggccggg catcgtatct ccactgccgc
     4441 cgatccgcgg acggccttgg agctgttacg gaatgagcca ttcgatctgc tcattaccga
     4501 ctaccgcatg cccgagatca cgggactcga cctcattcgc gaagcgacgc gtttacatcc
     4561 cgatctgatc ggaattctga ttacaggatt cgtgtggcct gacgcggccg ctgaggcaac
     4621 ggaggccggt gcgtacgatc tgatgctcaa gccgctgaac attggagagg tctgtgtcag
     4681 ggttcggaac gcctgtgaac gaatccggct ggtacgagaa aaccgccgct gtcaacgtga
     4741 gttggaacag gcgatgcgga tgactacgcg agatgctcag cgaatgacac cgtcagaggc
     4801 tattgcggag atcggtcttt tcccgcccaa cctgtcttct gcccctaagg ggacgcggaa
     4861 ggaagagcag atgctgaacc aattggaacg actggggaag ctctatcaga tgggcctcct
     4921 gacgaaggag gagttcgttc tgagcaaaag caagttgttc ggccggacct aacctcatgc
     4981 gaggattcgc tatggcagag gagttgcggg tgttggttgc tgaggacaat ccggatatcc
     5041 tggagctcct tggagaactg ctggaggcgg aacggtgccg ggtgactctg tgctcggatg
     5101 gggcgtcagc cctggcgcag atcgctgcgg agccgtttca cctcattctg agcgatatca
     5161 cgatgcctgg cgcctccggc ctcgagattc tcgaccgatc gagcaccatg gccgcaggca
     5221 ttccggttgt tctgatctcg gggaacgtcg atctggaggc cgcgcttttt gctctccggc
     5281 gcggcgcctt cgactgtctg ctcaagccat ttcgcctgga cgacatccgg gatctcgttc
     5341 ggcgcgtcca gcgactgcgc ggcctgacgg agaacggggc cggggagctg gtggccagag
     5401 cgtcgagacc gaacggtcat cggcctcaag agatcctgat caggaagagt cgagccctcc
     5461 ggttgattgc acagtccggt agggtgttgg gtggaaccca cgacctcgga cgactcatgg
     5521 caacaatcct ggagctggcc atgcggggta cgggagcaga actgggggca ctcttagtca
     5581 tgtcggaggc tgaagagaga ctgagggtta ccgcgagtaa gggcttcggc gataccgtga
     5641 agcctggcga tgaggtagag agtatagacg aactttggat tgtcgcaggt cttccgccag
     5701 ggagtgcgtc aaccgtggtt ccgctgatcg tgaaggggcg gcacgtcggc ggaatggcac
     5761 tggccagccg gcacaggtcc ggttcattct tagtggcgga ctgcgagatg ctggatatcc
     5821 tcgcccaaca agcttccatg gcgatcgaga atgtatccct ctacacggcg gtcgagtcga
     5881 gcgtctttga gggaatgcgc gccctggttg caacgcttga ggccaaggat ccgtataccg
     5941 agggacactc gttgcggttg gccaaggctg ctgtggcgat ctgtcggcag atggagctgg
     6001 gaagccacgt cgaaaatctg gtccgatacg ccggggctct ccacgacatc ggcaaggtcg
     6061 ggattcctga tgccattcta aggaaacccg gacggcttac ccctgaggaa tacgaacgaa
     6121 tgaaagaaca cccggtattg agctggaaga tcctgctgcc gttcagtttc cttcaagaag
     6181 aggcgataat tgttcgacac caccacgagt ggatcaacgg taatgggtat cctgacggac
     6241 tttccgggtc ggcgctcccg ctggcgtcca gaatcctggc ggcagccgac gcctacgatg
     6301 ccatgaccac cacacgccca tatcgaacgc cacgagcgcc tgagcaggcg attgaagaac
     6361 ttcgtcgttg tgcaggcttc cagttcgaca gcgatgtcgt tgaagcctta gccgcactct
     6421 ctccttgtcc gtatcacctg gacccaagcg atctttcccg gtagtgcccg agtagagaca
     6481 gcgatcccca gcgcgctggc agggtgtcca cgggaggaaa aaagttcgcc tcttgcccgt
     6541 catcctcaat ccccgttgat attcgctaga tgccggagaa cctagtaaat aatagctaat
     6601 tcggttagca aataatactt agtgcttgac gacctggaaa acacgtcata atatactacg
     6661 gttattttgg agaccgttta gtatcagatg tgagtccagg cagtgggtag atccgtcatt
     6721 gcctcctctc gcgcgagaga ggagatacgt gaggtagagc tttcggctta tggatcgggt
     6781 tgcggtgctg ggggagcaga gatccacgat agatctggtg gtaaccgctt tcgagcacca
     6841 aggagtagaa gtcagtagaa ttgagcacca atccgacgtt gcggcaactc tcgcgaacaa
     6901 gccttatctc ctcgtgatct tttgcctgga gaagatggag agtacggatc tgaatggtct
     6961 ttcccgcctg gtcgccgcct ccgccgacac gccgatcgta ccggccgtgt tcacactaag
     7021 cctcgaaatg gcgctcgcag cgattcgtct cggggcgttc gatgtgctgg tccttccttc
     7081 aactacagag gccgtaagga acctcctggt cagggc
//
LOCUS       DATMXI010000125         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_188158, whole genome shotgun sequence.
ACCESSION   DATMXI010000125 DATMXI010000000
VERSION     DATMXI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188158"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..826)
                     /locus_tag="VN444_02295"
     CDS             complement(365..826)
                     /locus_tag="VN444_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20674.1"
                     /translation="MKQILTFTGAVTSALLFSTWAFAQYQPPVIPTVPTISDIKTGVE
                     QKAAEERGKAEGKAKGAIERAEEKSKNAADKAAMKAEQAQKKAQQETEKAAAKVEQRK
                     RKAETKAIQTQQKVKEQTEKAHDTAVGHVEKAETKTSQEIEKAERKLLLPR"
     gene            complement(1237..2076)
                     /locus_tag="VN444_02300"
     CDS             complement(1237..2076)
                     /locus_tag="VN444_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HWR20675.1"
                     /translation="METRLIRIGHSPDPDDAFMFYALAKERLDTGQFRFQHILEAIDV
                     LNGWAMEGRLEVTAISAHAYAYVSDRYILLPHGASIGRNYGPILVAKGALDPADLRGK
                     RIAVPGRLTTAFLVLQLYLSEFQAVEVPFDQVFDAVERGEADAGLVIHEGQLTFETSG
                     FTKLLDLGTWWYEQTALPLPLGANAIRKDLGEPCCLEVSGYLQASINYGLAHRQEALQ
                     YAMQFGRGLDVALTDRFVGMYVNEDTRTWNQETRRGLEHLLNLAWEQGLITKQIRPEF
                     LPG"
     gene            complement(2091..2786)
                     /locus_tag="VN444_02305"
     CDS             complement(2091..2786)
                     /locus_tag="VN444_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HWR20676.1"
                     /translation="MPTSAKEQFINFAFYKVDSAWRRLPKEEREVSKKEFSSAVEQWH
                     EQMLLIPYSTVGLRPDTDFMLWRIGGSLKLLQEMSRQLNGTALGAYLTTPYSFVAMSR
                     RSTYVAKHLHPGQEGRRATIIPGDHQYLFVYPFVKQRDWYLLPLDRRQEMMKVHIEVG
                     HKFPSVKLNTTYSFGLDDQEFVVAFESDRPADFVELIMALRETEASRFTLRDTPIFTC
                     IQKTIHETLNDLG"
     gene            3271..3552
                     /gene="rpsU"
                     /locus_tag="VN444_02310"
     CDS             3271..3552
                     /gene="rpsU"
                     /locus_tag="VN444_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWR20677.1"
                     /translation="MALDDGTEEASGGSRYDKGTRHRPLEVKVDGRGVESALRLFKKL
                     ILRDGILKELKRRAHYEKPGEKRRRKVREAARRLRRQAARAVRRDPAEF"
     gene            3672..>3940
                     /locus_tag="VN444_02315"
     CDS             3672..>3940
                     /locus_tag="VN444_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWR20678.1"
                     /translation="MGTRVYVGNLPFDTDASALRALFEEGDRKVTDVKIITDRDTGRP
                     RGFAFVEMESADDAQAAIQALNGRDVGGRALTVNEAKEQAPRRSGG"
BASE COUNT          813 a         1073 c         1148 g          906 t
ORIGIN      
        1 ttgctataca tagcgtcgta agtcgcaaca atgaacggca ttcccgcgaa gcttgccccc
       61 gcatgtttta agtggggagc gggaatccag aaaggcactg gattcccccc gtattaagta
      121 cggggcaggc ttgtcaagcc cggaatgaca aactgcctga gacttatgtc gtcgtgtata
      181 gtacttggtt gcggaagtta gcgtgtatac attgtgagga cgcccttcgg cccgcccgca
      241 tcggttacgc catcggtggc ctcagcctca tgatccgatt tgtcatgcca ccgtcattgc
      301 atcttacggg atcgccaagg ttatgacgcg caaggaacga tacagattat ctacgattcc
      361 cttcttaccg agggagcaag agtttccttt cggccttctc tatctcctgt gaggtcttcg
      421 tttcggcttt ctctacgtgc cctacggccg tgtcgtgcgc cttctctgtt tgttccttga
      481 ctttctgctg tgtctggatc gccttggtct ccgctttgcg cttccgctgc tcgacctttg
      541 ccgccgcctt ctccgtctcc tgctgggctt tcttctgcgc ctgttcagct ttcatggctg
      601 ccttgtccgc ggcgttcttt gatttctcct ccgccctctc tatggcccct ttggccttcc
      661 cttccgcttt cccccgttcc tccgccgcct tttgttcaac cccggtcttg atgtccgaga
      721 tcgtcggtac tgtcgggatg acgggcggtt ggtattgggc aaaggcccac gtgctgaaca
      781 gcagcgcgct cgtgacggca ccggtaaatg tgagaatctg tttcatctgc gagccctcct
      841 tggtgagaat gctgttgatg aacgctacgc caactagtgt agtgtcccag tgataggtta
      901 acaatgaacc cgttcgggct gagcctgtcg aagcctgtcc tgagctaagc cgaagggccc
      961 agtttacgca cccttcgaca agctcagggc gaacggagct gtaaaggtat ttacgggaca
     1021 ctacactaga gtgtaagaac tttcaccacg gtgtttctct atcgagctga gcatagacaa
     1081 ggcattgcac ggtgtcaaga gtggtgattg gggaggggtg gggagagcgt gggaaggcat
     1141 ggaatagcga ccagatgcgt tactgagaag tacgacttgc cgacggtagt gggtcggatc
     1201 ggatcgaggg tgtaaagcaa cgaggggagt gggggcttaa cccgggagga actccgggcg
     1261 gatttgcttg gtgatcaggc cttgctccca tgctaaattc aggagatgct cgaggccccg
     1321 cctggtttcc tgattccagg tacgggtatc ctcgttcaca tacataccga cgaatcgatc
     1381 ggttagagcc acatctaatc cacgaccaaa ctgcatagcg tattgaagcg cctcttgtcg
     1441 gtgagccagg ccatagttta tactggcctg aagatatccc gaaacttcca gacaacacgg
     1501 ctcacccaga tccttgcgga tagcattggc ccccagaggc agcggcagcg cggtctgctc
     1561 gtaccaccag gttccgagat ccagcaactt ggtaaaaccg ctggtctcga aggtgagctg
     1621 cccctcgtgg atgacaagtc cggcatccgc ctctcctctt tcgaccgcgt cgaagacctg
     1681 gtcgaagggg acctcgacag cctgaaactc actcaggtag agctgcagca caaggaatgc
     1741 cgtcgtgagt ctaccgggaa cggcaattcg cttgccacgg aggtcggccg gatcgagcgc
     1801 gcccttcgcc acgaggatcg gcccgtagtt ccgtccgatg ctggcgccat ggggcagcag
     1861 aatgtagcgg tcagacacat acgcgtacgc atgggctgag atcgctgtca cctccagcct
     1921 gccttccatc gcccaaccgt tcagaacgtc gatcgcttcg aggatatgct ggaagcgaaa
     1981 ctggcctgtg tcgagtcgct ccttggccaa ggcgtaaaac ataaatgcat catcagggtc
     2041 cggactatgt ccgatccgga tgagacgtgt ctccatgcgt tgtccgcttt ctagcccagg
     2101 tcgttcaggg tctcgtgaat ggtcttttga atgcaggtga agatcggcgt gtcgcgaagg
     2161 gtgaaacggc ttgcttcggt ctcacggagt gccatgataa gctccacgaa atcagccggc
     2221 cggtcgctct caaacgcgac cacaaactcc tggtcatcga ggccgaatga gtaggtggtg
     2281 ttcagtttca ccgatggaaa cttgtggccc acctcgatat ggactttcat catctcctgt
     2341 cgccgatcga gcggcaacag gtaccagtcc cgttgcttga cgaatggata gacgaatagg
     2401 tattgatggt caccggggat gattgtcgct cgacggccct cttggccagg atggagatgc
     2461 ttggccacat aggtcgagcg cctggacatg gcaacgaatg agtagggcgt cgtcaggtag
     2521 gcgccaagcg ccgtcccgtt gagctgtcgt gacatctcct gcaacagctt cagcgagcca
     2581 ccgatccgcc agagcataaa gtcggtgtcc ggtcgcagcc ctacagtgga gtaggggatc
     2641 agcagcatct gttcatgcca ctgctccacc gcggagctga actccttttt acttacctcg
     2701 cgctcttctt tcggaaggcg tcgccaagcc gagtcaactt tgtagaaagc gaaatttata
     2761 aattgttctt tggccgaggt cggcatgtca tcgtcctccg taactactta ggtagataga
     2821 ctgtagtctt ttagactgta ggagcgcgcc atgcgagacc ataccaagct cagagcgttt
     2881 gaactagccg acgaagcgcc ttgattcggt ccgtgcgcaa cgactaacag cctttagtct
     2941 acagcctaaa cacctgagtc gttaccgtcc tccttcatcc tacttcgggt tgggccgccg
     3001 cccccgctgc gccacgcgtg tcaccttcgc cacatccctt atatacatca ggatcagggt
     3061 gggcgcaagg actggtttag gtgtcgaccg tggtgttggc tgattatcct tgacacatct
     3121 gccggggatg cttatactca gtcgattttt ggtctgaggg ctgcatttgc gtttactgaa
     3181 aacaccgcga ggaccagggg ctcgcggtat tttcgtttct aagaccgtga attatgtcag
     3241 cgggaatcga cactgtgatg aagagggata atggctcttg acgatggtac tgaggaagcg
     3301 agcgggggtt cgaggtacga taaagggact cggcatcgcc ccctcgaggt caaggtagat
     3361 ggccgagggg tcgaatcagc tcttcgactc ttcaagaagc tcatcttgcg cgatgggatc
     3421 ttgaaggaac tgaaacgaag agcacactac gaaaaaccag gggagaagcg tcgccgaaag
     3481 gttcgcgagg ccgcacgcag acttcgccgc caggcggcgc gggcggttcg ccgcgacccg
     3541 gcggaattct gagtcccccc gggaggtccg ggtggatgaa gccagccatg gaaggcggga
     3601 ccatggcgat ggcgctaatt gaactacccg tgagaagtcg gccgcaggcc gacggaagga
     3661 gtttcgaaag catgggaaca cgagtgtatg ttggtaatct cccgtttgat acggatgcat
     3721 ctgcgctccg cgccctcttc gaagagggtg atcgtaaggt caccgatgta aagatcatta
     3781 cagatcggga tactggacga ccccggggct ttgcctttgt cgaaatggag agcgcggacg
     3841 acgcacaggc cgctattcag gccttgaacg gacgagatgt tggtggtcgg gcgctgacgg
     3901 tcaatgaggc aaaagaacag gctcctcgcc gaagcggtgg
//
LOCUS       DATMXI010000126         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_143776, whole genome shotgun sequence.
ACCESSION   DATMXI010000126 DATMXI010000000
VERSION     DATMXI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143776"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1775)
                     /locus_tag="VN444_02320"
     CDS             complement(222..1775)
                     /locus_tag="VN444_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWR20679.1"
                     /translation="MNGSTRSGTLSFVVVILLGTFLCLLSLDGRAFAQSEEDQRAGFG
                     ALNSLSSERMIADVRTLSSPAFNGRQAGSADDLRSAQWVAQELTLAGVQLPLIDNKGI
                     AFPSPGASKEEPAGIMASMVSTPLIEPDPVVRTGATDQLVTAQLGRDYLPVFDSPSAD
                     LQGQVVFVGYGIVDPAQGIDDYAGVDVKNRIVLFLRGKPDHYPSPVSHADKVRFARAR
                     GALAYLTATGPIISPYEIRRGATGRPSALYGQLSPDQALPGACISTKLAETLLAGSSD
                     ESNPDHLRTLQEQLNNAPAARSRLVNRYASLHWKTTIADGLLTNVVGMIPGTGPDTIV
                     IGAHRDHFGRPAGLLFPGADDNASGTAIVLEVARALGKIGLRPQRTILFLSFSGHERD
                     SLGSRLYTSRPVIPLGSTKAMINIDHVGVGDGVLILRVTEFKKSTLKEAGYAVGLVRK
                     LDYYGFLPGGDDEPFKEAGISTVSIASGGAHPHMHQPTDTADTINPEILRNIARYVLA
                     LTWQLANAQ"
     gene            1882..>2012
                     /locus_tag="VN444_02325"
     CDS             1882..>2012
                     /locus_tag="VN444_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HWR20680.1"
                     /translation="MTDPALPIDRSPNRLITEASPYLRQHAKNPVDWYPWGKEALSRA
                     "
BASE COUNT          439 a          548 c          593 g          432 t
ORIGIN      
        1 gactgatcca aggcgccaat cagaccggct caccgatcgt ccttcccggt cctccacgcc
       61 aaaccgcgcc gttgccatcg attcagtcga cgattctcga tgacatcccc tgacacaatg
      121 ctgaaaaaga ccgccggctt ctaagaaggc taaggtcaag gttggaatga gaagaaaatg
      181 atcttcggtc aaccacgacc tcgacctgag ccggtggcat tctattgagc attcgccagt
      241 tgccaggtca aggcgaggac ataccgtgct atgttccgca aaatttccgg attgatcgtg
      301 tcggcggtgt cggtaggctg atgcatgtgc gggtgggccc cgccgcttgc gatgctgacg
      361 gttgaaattc ctgcctcttt gaacggttcg tcgtcgccgc ctggcaagaa tccgtaatag
      421 tcaagctttc ttaccaaacc cactgcgtac cctgcttcct ttaacgtgga tttcttaaac
      481 tctgtgacac ggaggattaa cactccgtct cccactccga catggtcaat gttgatcatg
      541 gctttcgttg agccgagggg gatgacaggt ctcgatgtat agaggcgtga cccgagcgaa
      601 tctctctcat gtccactgaa tgataaaaag aggatcgtcc gttgaggacg cagaccgatt
      661 ttccctaggg cccgtgcgac ctccagtact atggccgttc ctgatgcatt gtcgtcagcg
      721 ccaggaaata agaggcctgc agggcggcca aaatgatctc gatgcgcgcc gatgacgatg
      781 gtatccggcc ctgtaccagg aatcatcccc accacgttcg tcagcaagcc gtccgctatc
      841 gtggttttcc aatggagaga tgcatatcga ttgactaggc gcgatcgcgc tgctggggcg
      901 ttattgagct gttcttggag ggtgcgaagg tggtcaggat tggactcatc gctgcttccg
      961 gcgaggagag tctcggccaa tttcgtgctg atgcatgcgc cagggagggc ttggtcaggg
     1021 gagagctgcc catacaatgc gctgggcctt cccgtggcgc cgcggcgaat ttcatatggg
     1081 ctgataatcg gccctgttgc ggtcagatag gccaaggctc ctcgagctcg tgcgaagcgg
     1141 accttgtcgg cgtggctgac agggcttggg tagtgatccg gtttgccacg caggaagagc
     1201 acgatgcggt tcttcacatc gacgccggcg taatcgtcga ttccttgagc gggatcgaca
     1261 atgccgtaac cgacgaagac gacctgaccc tggagatcgg cagaggggga gtcaaagacc
     1321 gggaggtagt ctctacccaa ctgcgctgtg accaactggt ccgttgcgcc ggtgcgaaca
     1381 accgggtcgg gttcgatgag tggcgtcgag accatcgagg ccatgatgcc agcgggctct
     1441 tctttgctgg ccccgggaga ggggaaggcg attcccttgt tgtcaataag cggtagttgt
     1501 acccctgcca aagtcaattc ctgtgcaacc cattgcgcag agcgaaggtc gtcggcggat
     1561 ccggcttgcc gtccgttgaa cgccggactg cttaacgtgc gcacgtcggc gatcatccgt
     1621 tcgctcgaca gactattgag tgcaccaaac cctgcacgct ggtcttcctc cgattgtgcg
     1681 aaggcgcgcc catcgaggga aagcagacaa aggaaagttc ccaggagaat gacgacaaca
     1741 aaggagagag tgccgctgcg cgtcgagccg ttcatgcagt gaacctttta aggtaatgca
     1801 gaactataac atagtctgac tggttcattg cagagcagcc tgtagcaagg tactatggcg
     1861 aatgatggga acgaagaagc gatgacagat ccagctctac ccatagaccg atctccgaac
     1921 cggctcatca cagaagccag tccctatctt cgccagcatg cgaagaatcc ggtggattgg
     1981 tatccctggg gcaaggaagc cctgtcacgg gc
//
LOCUS       DATMXI010000127         6265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_206915, whole genome shotgun sequence.
ACCESSION   DATMXI010000127 DATMXI010000000
VERSION     DATMXI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206915"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VN444_02330"
     CDS             <1..677
                     /locus_tag="VN444_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HWR20681.1"
                     /translation="LGGMDGGGFGLMDMVILGAIGFAIYWVVKQRRQAAPVGGPYQRV
                     SVGQADAAWGERGQERYQGAASQATIVQEPDLDQGLAHIRQMDPGFEEGRFREACTDL
                     FFKVQAAWGNRDLESVRAILTPQMYSQLAEDVGRLKTERRINRLENIAVRSVELTEAW
                     QEKGQDYVTVRFLANLLDYTVDEGTSQVVEGSRTNPVKFEEFWTVMRPVGPNPWILSA
                     INQAEN"
     gene            complement(905..1108)
                     /locus_tag="VN444_02335"
     CDS             complement(905..1108)
                     /locus_tag="VN444_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HWR20682.1"
                     /translation="MRVKGKIKWFNDSKGYGFIERPDGDDVFVHFSAIQGSGFKTLAE
                     GQEVEFEVVDGPKGKQAANVVGL"
     gene            complement(1568..3895)
                     /locus_tag="VN444_02340"
     CDS             complement(1568..3895)
                     /locus_tag="VN444_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR20683.1"
                     /translation="MTTSGRSAALAIKEPMHQCAESQQPLLLPQANKYRTLRIVLIKP
                     SQYDDDGYVIRFWKGVLPSNTLSVLRGLTEDIERRRALGDIAIQVDTFDEVAEKVPVE
                     QIVTWNRHPSTKLVVCLVGVQTPQFPRALALGKQFREYGIDVMIGGFHTSGTINMLGD
                     QGPDIQELYRESIVVVSGEVEGHWEGMLADLLNGQLKPLYSFAQDLKSLVDIEDAPLP
                     VVSPKTMKHFARPNFGTVETSRGCPFSCSFCTIINVQGRMMRERSPEAITELIRRNFA
                     ERDINFYFFTDDNFARKKWWRETFEAIIRLREEGIKITFMMQVDLARKPQEFVRLAAE
                     AGCTQVFVGMESVNPHNLKVQGKGQNKVEEYQAIIREWHDAGIAVHVAYIIGMPFDTK
                     AQVALDIQYLIDEIQADQASFFMLTPMPGSQDHKEMQEHGEWMDPDLNKRDSFHATTA
                     HPYMTTEEWTAAYEAAWKSFYSKENLARVLSRWSHAPQQYWNLFNVYVWYKNAALIEK
                     QHPMVAGFFRLKGRLTRRSGFSIDSLPVHLWKRTKEVCRLFVTWAHFVKEMEEVWLQT
                     RPRSEREKQFFDEVQRIQGEIWQTLKIAEWQKAYSDAKTAMPAKVRALLDPFEDLSSK
                     LLLSRNDLNAFLKKWGSLQAKIQTLHRGWAWGDGPAKKWLEQLHTLHRDAWQSVKVRE
                     WQEAYSGFKGGLPSRLDLLYARFDALSNRMVCSRQDLNTFQARTRGHLSGMRFWYIRP
                     FRLAGTFFKEFLLIIAFARSVFASLRSSLPRATQH"
     gene            complement(4029..5027)
                     /locus_tag="VN444_02345"
     CDS             complement(4029..5027)
                     /locus_tag="VN444_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWR20684.1"
                     /translation="MRALCVNSTRPIEDRPLALIELPTPIPGPGELRLRVQACGLCRT
                     DLHIIEGDLPLPTLPIVPGHQIVGTVDQVGEGVTHYQAGDRLGVPWLYSTCGRCAFCR
                     RDQENLCDAARFTGYHVNGGYAEFVVAPEASAYPLPTGISTAHLAPLLCAGVIGFRAL
                     RLSEVRPGERLGLYGFGSSAHITIQVAVHWGCEVYVFTRSEAHRALARQLGAHWAGRV
                     EDELPGLLDSAVIFAPVGRLVQQALRILRKGGTIALAGITMSPIPELDYALLYQERTI
                     RSVANSTRQDVRDLLRLAVDIPIRTEVRVFPFEEANHALQLLKQSRFSGAGVLVIS"
     gene            complement(5050..5799)
                     /gene="recO"
                     /locus_tag="VN444_02350"
     CDS             complement(5050..5799)
                     /gene="recO"
                     /locus_tag="VN444_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWR20685.1"
                     /translation="MPLQTTEAIVIGGHNLGEADRIIPFFTRQLGKVRAVARGARRIR
                     SRYGGSLELFTLGQLVFFEWPNRTLHKINEFSVIEPFVELKADLGRLGRGAYLVELAG
                     ASVEEAEPNEEIFLLLRDALTLLALHDDPRLLRAFEIRLLRIVGYLLELYRCLICQST
                     LEHSAEPAISPTRGGLVCSRCRTRAQDNLPISLESLGFLRSALHGGLEQSLASPLVHP
                     HTASLQEVLRVCIAHFFGKRLRSIAFLNLVE"
     gene            complement(5844..>6265)
                     /gene="era"
                     /locus_tag="VN444_02355"
     CDS             complement(5844..>6265)
                     /gene="era"
                     /locus_tag="VN444_02355"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWR20686.1"
                     /translation="ALLESLLVQYLPEGPPLYPLDQLTDQPEWFIIAELIRERVFRLV
                     YQEVPYAVAVLVEQVRMREGRALTDVEATIYVEKDSQKAIIIGRGGAMLKKIGELARP
                     EIEKLLGTQVFLKLWVKVRSDWQTDDEALKRLGYLQS"
BASE COUNT         1342 a         1834 c         1774 g         1315 t
ORIGIN      
        1 gccttggcgg gatggatggt ggtgggttcg gcttgatgga catggtgatc ctgggcgcga
       61 tcggcttcgc gatctattgg gtggtgaagc agaggaggca ggcggcgcca gtcggggggc
      121 catatcagcg cgtgtcggtc ggtcaggcag atgcggcgtg gggcgagcgc gggcaggaac
      181 gctaccaggg ggcggcttcg caagcaacaa tagtccaaga acccgatctg gaccagggct
      241 tagcgcatat ccgccagatg gaccctggtt ttgaggaggg gcgctttcga gaggcgtgca
      301 ccgatctctt tttcaaggtc caggcggctt gggggaatcg tgatcttgaa tcggtccgtg
      361 cgatcctcac gccccagatg tactcccagc ttgcagagga cgtggggcgg ctcaagacgg
      421 aacggaggat caaccgcctg gagaatatcg ccgtccgctc cgtcgaactg accgaagcgt
      481 ggcaggaaaa agggcaggac tatgtcactg tccgattcct ggcgaacctc ctcgattaca
      541 cagtggacga agggacctcc caggttgtgg aaggaagccg gacgaatccc gtgaagtttg
      601 aggagttctg gactgtcatg cgcccggtcg gtccgaaccc ttggatcctc tcggccatca
      661 accaggcaga aaactgagcg ttcagcaatg aactaccccg cgcagccttc ggggcatcgt
      721 cttctgttct ggcccgtcat tccgtgcttg acacggaatc cagtctggcc ctctggatac
      781 ctgcttccgc cggtatgacg aactcacggc aagccgcggg gaatgaaccc ctagttgtgg
      841 agtcaacaaa aagcccccgc gagtttcctc gcgggggctt tttgttcgtt actgaggatc
      901 gtcgttaaag tccgacgacg ttcgcggcct gcttcccctt tggcccatcg acgacctcaa
      961 actcgacctc ctgaccctcg gccagcgtct tgaagccgga gccctgaata gcggaaaaat
     1021 gtacgaacac gtcatcgcca tccggtcgct caataaagcc gtaccccttg ctgtcattaa
     1081 accacttgat cttgcctttt accctcactt ctttcttccc tcctttttca tagctctctc
     1141 aggaccgcca gaaggtcttc ccgactatgg gggccctggc agaccagaga gatggttcct
     1201 ctctacgcat gcgactcccc gttcgcactc cggtacattc catgtaacta ctcaggtagt
     1261 taggctgaag actaaaggct tttaggagta agccatgcgt tcagacaaaa ctccgaaaag
     1321 gtcttgaatg gctcgatttg tgccttgcga gatcattgag aaccttcagc cttcagtcta
     1381 aacaccttgt agttaccaca ttccatagca gcgagactac aaggccatcg gtgagcctgc
     1441 tgcgagaagt ctgtccccga aatcgttgac atataaccat acttgatatg tattgtcaag
     1501 atgatgatgc cgaattacgt caaggcgagt ctgaaagctt tcgggatgac gaccgcctcg
     1561 catcacgtca atgctgcgtc gccctcggca gtgacgagcg aagcgatgcg aagacgctcc
     1621 gagcgaacgc gatgatcagc agaaactcct tgaaaaaggt acccgcaagc ctgaagggcc
     1681 ggatatacca gaatcgcatc ccgctcaggt gcccccgtgt tctggcctga aacgtgttga
     1741 gatcctggcg ggaacagacc atccgattgc tcaaggcatc aaacctggca tagagtaaat
     1801 ccagcctcga cggaagtccc cccttgaaac cggagtaggc ctcttgccat tcgcggacct
     1861 tcacgctctg ccaggcgtct cgatggagag tgtggagctg ttcaagccat ttcttggcag
     1921 gtccgtcccc ccatgcccag ccgcgatgga gcgtctggat cttggcctga aggcttcccc
     1981 acttcttcag gaacgcattc aagtcattgc ggctgagcaa cagcttggag gagagatctt
     2041 caaagggatc gagcagggcc cggaccttag cgggcatcgc cgtcttggca tcactatacg
     2101 ccttttgcca ctctgcgatc ttcaaggtct gccagatctc tccttggatt cgctgaacct
     2161 catcgaaaaa ctgcttctcc ctttcactgc gcggacgggt ctgaagccac acttcctcca
     2221 tctccttcac gaagtgggcc cacgttacga acagacggca tacctctttc gtacgcttcc
     2281 agagatggac cggcaacgaa tcgattgaaa accccgatct ccgtgtaagc cggcccttta
     2341 gacggaaaaa gccggcaacc atcgggtgtt gcttctcgat caacgcagcg ttcttgtacc
     2401 acacgtacac attaaagaga ttccaatact gctgaggggc gtggctccat cgggagagga
     2461 cccttgcaag gttctctttg ctataaaacg acttccaggc tgcttcatac gcggcagtcc
     2521 actcctcagt agtcatgtag gggtgtgcgg tcgtggcgtg aaaagaatct cgcttattga
     2581 gatcggggtc catccactcg ccgtgttcct gcatttcttt gtggtcctga gatcccggca
     2641 tcggggtcag cataaagaag gaagcctgat ccgcctggat ctcatctatg agatactgaa
     2701 tgtcaagggc tacctgcgcc ttcgtgtcga agggcatgcc gatgatgtag gctacgtgga
     2761 cagcgatgcc ggcatcgtgc cactcgcgga tgatcgcctg atactcctct accttgttct
     2821 gtcccttacc ctgaaccttg agattgtgag ggttgacgct ctccataccg acgaacacct
     2881 gagtacagcc ggcctcggct gcgaggcgca cgaactcttg cggcttacgg gccaggtcca
     2941 cctgcatcat gaaggtgatt ttgatccctt cctctcgcag ccggatgatc gcctcaaagg
     3001 tctcgcgcca ccacttcttc cgggcaaagt tgtcgtcggt gaagaagtag aagttgatgt
     3061 cgcgttcagc gaaatttctc cgtataagct ccgtaatggc ttccggagag cgttcccgca
     3121 tcattcggcc ctgcacgttg atgatcgtac agaagctgca cgaaaatggg cagcctcgag
     3181 atgtttcaac cgtcccgaag ttgggcctgg caaagtgctt cattgtttta gggctaacga
     3241 caggtagggg ggcatcttcg atgtccacca ggctcttgag gtcctgagca aaggagtaga
     3301 ggggtttcaa ttgtccgttg aggagatcgg ccagcatccc ctcccaatgg ccttcaacct
     3361 cgccggatac gactacgatt gattctcgat agagctcctg gatgtccggc ccttgatcgc
     3421 cgagcatatt gatggttccg ctcgtgtgga atccgccaat catgacgtca atcccgtact
     3481 cacggaactg cttccccaaa gcgagcgccc tcgggaactg gggggtctgg accccaacca
     3541 ggcacaccac cagcttcgtg gaaggatggc gattccatgt gactatctgc tccactggca
     3601 ccttttccgc cacctcatca aaggtatcga cttggatggc gatgtcgcca agggcgcggc
     3661 ggcgctcgat atcttcggtt aagccccgga gtacactgag ggtattgctg ggcagcacgc
     3721 ccttccaaaa gcggatgacg tacccgtcat catcgtattg tgagggtttg ataaggacga
     3781 tccgaagagt cctgtacttg ttcgcttgag ggaggagaag gggctgttgc gactcagcgc
     3841 actggtgcat gggttcctta atggcgagtg ctgcactccg accagaggtt gtcatccctg
     3901 ttccttatat tctccggcgc cgttctcccg agaacatgac aatcacatta agagccgacg
     3961 ctgctaatta ccgtataaca aacgccccaa cctgtcaacg gagaaaataa aacccgaagc
     4021 aggtgacgct aggagataac caggacgccg gcgccgctga atcggctttg cttaaggagt
     4081 tggagggcgt gattggcctc ttcgaagggg aagacgcgga cttcggtccg gatcgggata
     4141 tccactgcca ggcgcaggag gtcgcgcaca tcctgtcggg tgctgttggc cacactccgg
     4201 atagtccgct cctggtatag cagggcgtag tcaagctccg gaatcgggct catcgtgatg
     4261 ccggcaagcg cgatcgtgcc ccctttcctg aggatccgga gcgcctgctg caccagccgc
     4321 ccgacaggcg caaagatgac cgcgctatcg agtagcccag gcagctcatc ctcgacccgt
     4381 cctgcccagt gggcgcctag ctgccgggcc agggcgcggt gcgcctcgct cctggtgaag
     4441 acatagacct cgcatcccca gtgaaccgca acctggatcg tgatgtgagc ggagctgccg
     4501 aacccataca gccccaagcg ctcgccgggc ctgacctcgc tcagccgcag ggctcggaag
     4561 ccgatgactc ctgcgcagag taagggggcc aagtgggcgg tcgagatgcc ggtcgggagc
     4621 gggtacgcag atgcctctgg cgcaaccacg aactccgcat accccccatt cacatggtag
     4681 ccggtaaagc gggctgcgtc gcagaggttt tcctgatcgc gccgacagaa ggcgcaccgg
     4741 ccacaggtgg agtatagcca gggaacgccc agccgatcgc cagcctgata gtgcgtcacg
     4801 ccctctccga cctgatcgac ggtaccgacg atctgatggc ctggcacgat ggggagcgtg
     4861 gggaggggca ggtcgccttc gatgatgtgt aggtcggtcc ggcatagacc gcaggcctga
     4921 acccgcaggc gaagctcacc cgggcccggg atcggagtcg gaagctcgat cagcgcgagc
     4981 ggacgatctt cgatcgggcg cgtactattc acacacagcg cccgcatcta cgccccctcc
     5041 actaacgccc tattcaacga ggttgaggaa cgcaatggat cgaagtcttt taccgaaaaa
     5101 atgcgcaata cacactcgca agacctcctg aaggctggca gtatgaggat ggacaagagg
     5161 tgaggcaagc gactgttcca gtccgccatg cagggccgag cgtagaaatc ccagtgattc
     5221 caaggagata ggtaagttgt cttgcgctcg tgtccgacat ctggaacaga ccagcccgcc
     5281 acgggttggg ctgatggcgg gctcagcact gtgctctagc gtagactggc atatcaggca
     5341 gcgatagagc tccaaaaggt atccgacaat cctgaggagg cggatttcaa aggctctgag
     5401 aagtcgcggg tcatcatgta gggcgagcag cgtaagggca tcccgaagta agaggaagat
     5461 ctcctcgttt ggctccgcct cctcgacgga tgcacctgcc aactccacga ggtaggcccc
     5521 ccggcccagt ctccccagat ctgctttgag ctcgacgaac ggttcgataa ccgagaactc
     5581 gttgattttg tggagggtcc ggttaggcca ctcgaaaaag acgagttgtc cgagtgtgaa
     5641 cagctcgagg ctcccgccat atcggctacg aatccgcctt gccccacgag caactgcccg
     5701 aaccttcccc aattgcctcg tgaagaacgg aataatccgg tctgcctcgc caagattgtg
     5761 tccgccaatc acaatcgctt cggttgtttg cagtggcatg ggtctaccaa aaggtgcgga
     5821 gggtggagcg ggcttcgcgc atgttacgat tgcagatagc caagtcgctt tagcgcctca
     5881 tcatccgtct gccaatcgct gcgaaccttt acccacagct tcaagaacac ctgggtgccg
     5941 agcagcttct cgatctccgg cctggctaac tccccgattt tcttcagcat tgcccccccg
     6001 cgcccgatta tgatcgcctt ctgcgaatcc ttctcgacat aaatagtcgc ctcgacgtcg
     6061 gtgagtgccc gcccttcccg catcctcacc tgctctacca aaacggccac cgcatacggc
     6121 acctcctggt agaccaagcg gaagacccgc tcacggatca gctcggcgat gatgaaccac
     6181 tcgggttggt cagttaactg atcaagagga taaagaggcg gtccctccgg gaggtattgg
     6241 accagcagcg actcaaggag cgcaa
//
LOCUS       DATMXI010000128         2180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_173209, whole genome shotgun sequence.
ACCESSION   DATMXI010000128 DATMXI010000000
VERSION     DATMXI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173209"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VN444_02360"
     CDS             <1..556
                     /locus_tag="VN444_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016724035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWR20687.1"
                     /translation="IDESALAVTWVVDFPLLEYDHDQGRWQAMHHPFTAPLDEDLPLF
                     GTDPGKVRAKAYDLVVNGQELGGGSIRIHRRDVQSQMFAALGIGEDEARAKFGFLMEA
                     LEYGAPPHGGIAFGLDRIIALLAGVTSIRDVIAFPKTQKAQDLMTKAPSSVDSRQLRD
                     LKIKLDLDAKGSVLAASFQPSTIS"
     gene            730..1722
                     /locus_tag="VN444_02365"
     CDS             730..1722
                     /locus_tag="VN444_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWR20688.1"
                     /translation="MTREQVLPGHKVSLSIGEEIQQLFGRKDEIRKLIEEALQVKLVA
                     RDGLVSIQGEASYVAVGEQVVAELISVLARGERVTPQDVKLALRLFIKKEKEEFRQIQ
                     GEVIDVSPKKRPVRPKGPGQRSYIEAIRHHDIVFAIGPAGTGKTYLAMAMAVSALLRH
                     EVSRIILTRPAVEAGEKLGFLPGTLYDKINPYLRPLYDALYDMVELERVTRLIEMGTI
                     EIAPLAFMRGRTLNDAFIVLDEAQNTTSEQMKMFLTRLGFGSKTVITGDITQVDLPTG
                     RLSGLIEVQRILKGIEGIKFAYFGEEDVVRHELVQQIVRAYEAYQAATLPAEGQ"
     gene            complement(1797..>2180)
                     /locus_tag="VN444_02370"
     CDS             complement(1797..>2180)
                     /locus_tag="VN444_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20689.1"
                     /translation="PTTNSPHSAPSTPTVANVACPTFFRKFVSSLIFLCRFVTCNAIR
                     PPSTGRLLKGKSQILLETPKREADQEVDPEERKDPRLECRGRRYEKNDRENSQDGEDV
                     DRAWMVLKAPSGPTEPGGHRTRSDG"
BASE COUNT          447 a          604 c          697 g          432 t
ORIGIN      
        1 gatcgacgag agcgcgcttg cggtcacatg ggtcgtcgac tttccgctcc ttgaatacga
       61 tcatgatcaa gggcggtggc aggcgatgca tcaccccttt acggcgccac tggacgagga
      121 tctgccgctc tttggcaccg atcccgggaa ggtccgcgcc aaggcctatg acctggtcgt
      181 gaacgggcag gagcttggcg gcggcagcat caggatccac cgccgggatg tccagagcca
      241 gatgttcgcc gccttgggaa tcggtgagga tgaggcgaga gccaagttcg ggtttctcat
      301 ggaggcgctt gaatacggcg ctccacccca cggcgggatc gccttcggcc tggatcggat
      361 catagcgctg ctcgctgggg tcacctcgat tcgggacgtc attgcctttc ccaagaccca
      421 aaaggcgcag gatctgatga cgaaggcccc ctcgtctgtc gactcgaggc agctcaggga
      481 tctgaagatc aaactggatc tcgatgcgaa gggcagcgtt ttagctgcca gctttcagcc
      541 ttcaacgatt agctgatcgc tgacagctaa ttgctgcccc tgttgacagg tccacggggt
      601 ggagatatgc ttgatgtagg ccgaaggagc agcttgaagc tgtcagcctt gagcgataag
      661 aggatcgtaa gttgctgacg gctgatcgct gatagctgaa cgttgcgtga tgagtagcga
      721 agcgacgaga tgacgaggga gcaggttctg ccggggcaca aggtctctct gtccatcggg
      781 gaagagatcc agcagctttt cggtcgcaag gacgagatca ggaagctcat cgaagaggcg
      841 ttgcaggtca agctggtcgc gcgagatggc ctcgtgagca tccagggtga ggcttcatat
      901 gtggctgtgg gagagcaggt tgtagcggag ttgatctcgg tgctggcgcg cggcgagcgt
      961 gtcacccctc aagatgtgaa gctggccctg cgcctcttca tcaagaaaga aaaggaggag
     1021 ttcaggcaga tccagggtga ggtaatcgat gtttcgccaa agaagcggcc ggtccggcca
     1081 aagggccccg gccagcgaag ctacatcgaa gccatccgcc atcacgacat tgttttcgcc
     1141 atagggccgg ccgggaccgg taagacgtat ctggccatgg ctatggccgt gtcggccctc
     1201 ctgaggcacg aggtcagccg gatcatcctg accaggcccg ccgtggaggc gggggaaaag
     1261 ctgggtttcc ttccaggcac actctatgac aagatcaacc cctaccttcg acccctgtac
     1321 gacgcgctct acgatatggt tgagctggag cgcgtcaccc gtctgattga gatgggcacc
     1381 atcgagatcg cccccctggc cttcatgcga ggccggaccc tgaacgacgc gtttatcgta
     1441 ctggacgaag cccagaatac gacctcagag cagatgaaga tgtttctgac gcgccttggc
     1501 ttcggttcga agaccgtcat cacgggggac attacccaag tcgatctgcc gacaggcaga
     1561 ctctccggcc tgatcgaggt tcagcgcatc ctgaaaggga tcgaggggat taaattcgcc
     1621 tatttcggtg aggaggacgt ggtgcgacac gagttggtgc agcagattgt cagggcctac
     1681 gaagcgtacc aggccgccac cctaccagcc gaagggcaat aggatggcgc cagatggcgc
     1741 cctatagggc cggcggcgac ccaatcacca ccgtgcagtt atcggcgaca cgcaggctag
     1801 ccatcgctcc gcgtgcgatg gcctcctggt tccgttgggc cgctgggcgc cttgagcacc
     1861 atccaggcgc gatccacatc ctctccgtcc tggctgttct cgcgatcatt cttctcatag
     1921 cgtcttcccc ggcactccag gcggggatcc ttccgctcct cgggatctac ctcctggtcg
     1981 gcttcccgct tgggagtctc gaggagtatc tgggatttgc ccttgaggag tctgccggta
     2041 gaagggggac gaatggcatt acaggtcaca aaccgacaaa ggaagatcag gctcgatacg
     2101 aactttctaa aaaaggtcgg acaggctaca ttagccacag tgggggttga aggggcggag
     2161 tgcggactgt tggtggtggg
//
LOCUS       DATMXI010000129         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_322942, whole genome shotgun sequence.
ACCESSION   DATMXI010000129 DATMXI010000000
VERSION     DATMXI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322942"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..633)
                     /locus_tag="VN444_02375"
     CDS             complement(193..633)
                     /locus_tag="VN444_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20690.1"
                     /translation="MDGEDEATIGASGSFASGDGKANKSLEAMMATALGLFSAFADFV
                     KTLSVSVPALQFIGIVLVAFLLGLLLGRITKRVGQQAVGNSNEREVGRVVLDFERLTP
                     LGPAEEEEVDNSVMDISNLRPDQMPERPAMSDREEQQEPTLLKE"
     gene            complement(791..1120)
                     /locus_tag="VN444_02380"
     CDS             complement(791..1120)
                     /locus_tag="VN444_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWR20691.1"
                     /translation="MRLPSRTSIVFIAVLATAAFPLLQACAPTATRESTGEYLDDSAI
                     TAKIKAKLLGDSTISGFAITVETFRGRVILAGFVNSQAQVDRAIALSREVAGVREVQS
                     ALVIKSR"
     gene            1254..2288
                     /gene="pdxA"
                     /locus_tag="VN444_02385"
     CDS             1254..2288
                     /gene="pdxA"
                     /locus_tag="VN444_02385"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWR20692.1"
                     /translation="MPRHTTFRPFLGLTIGDPAGIGPEIVAKAVTQEEVRAACRLLII
                     GEAEIMRRAIRLCHLDLLVRSITSPAEVTADPGCLEVLDLKNIDTANCPPGVLAPHCG
                     RAAVEYLNKAIDLAIARELDGVVTGPLNKEAMALAGFKYDGQTELFAERTGTKEYAML
                     LVVGRMRVLHVSTHTSLRTVCDKVKQARILTVIRLAHQVLCDLGSRKKRIGVAGLNPH
                     AGESGRFGREEIEEIMPAIEAARAEGIRVGGPFPPDTLFHRLKLGEFDAVVAMYHDQG
                     HVPLKLIGFHRGVNVTVGLPIIRTSVDHGTAFDIAGTGTANPRSLVEAILLATKFAHR
                     RRKTPRALDV"
BASE COUNT          525 a          760 c          696 g          505 t
ORIGIN      
        1 ctgtggtcct gatcgacacc gagcgctcca tcggctgtct gtgcaaagat ctccgcgagc
       61 gcactcgtca tcagactgat cgtctccatt gtgtgtgcca ttcggtgggt gagcaattga
      121 tcagggaacg tacctgctaa aggtaccgaa aactactgga aaaatcaagg aggcggcaaa
      181 ctgtgatgtg gatcattctt tgagtagtgt cggctcctgt tgctcctctc gatcactcat
      241 cgcaggtctc tctggcatct ggtcaggccg gaggttagag atatccatca ccgaattgtc
      301 tacctcttcc tcttcagcgg gaccaagcgg tgtcaacctc tcgaaatcta atacgacgcg
      361 gcccacttct cgctcgtttg aattcccaac ggcctgttga cccacacgct tcgtgatccg
      421 cccgaggagc aagccgagca gaaacgcgac gaggacaatt ccgataaact ggagcgccgg
      481 cacagaaaca gataacgtct tcacgaagtc agcaaaggcg gagaacaggc cgagcgcagt
      541 cgccatcatc gcctctaggc tcttgttggc tttcccatcg cccgatgcga agcttccgct
      601 ggcgccaatc gtggcctcat cctcgccgtc caagcaccag tggggttttc acacgactgt
      661 gggcaatagg aggttgcgtg aggcttacaa acaggtgggc ggtagtgtgt gagctggcag
      721 gaaagagaag cgggacgaaa acgccattac ggagcacgcc tctcatcatc aacagcggtc
      781 tcctcggtcg ctacctactc ttgatcacca gcgccgactg tacctccctg acgccggcca
      841 cttctctgga cagggcgatc gctcgatcaa cttgggcctg agagttgaca aacccggcca
      901 ggatcacccg cccgcggaag gtctctaccg taattgcgaa gccactgatt gtagagtcgc
      961 caagcagttt cgccttgatc ttggcggtga tagccgagtc atcgagatac tccccggtgc
     1021 tctcgcgagt ggccgttgga gcgcaagcct gaagcaaggg aaaagcagcg gtagcaagaa
     1081 cagcaatgaa cactatgctg gttcgactcg gcagacgcat cagtgcctcc tccggttact
     1141 cgattcgcca tcaaggacgc ctacaggctt agcaccatag ggtcactata ccagaaatgc
     1201 ctgtggagaa gaagcgcttt atgctatcat tcaactcact ttcaggagga atcatgcccc
     1261 ggcatacgac ctttcgtcca tttttgggat tgaccatcgg cgacccggca gggatcggtc
     1321 cggaaatcgt ggccaaggct gtaacacaag aggaggtgcg ggcggcctgt cgtctactga
     1381 tcatcggtga ggctgaaatc atgcggcggg ccatcaggct ttgccacctc gacctcctcg
     1441 ttcgatccat cacctccccg gctgaggtca cagcggaccc gggctgtctt gaggtactgg
     1501 acctgaagaa tattgatacc gcgaactgtc caccaggcgt tctcgctccc cactgcggca
     1561 gagccgcggt ggagtacctc aataaggcga tcgatctcgc gatagcgcgc gaactggatg
     1621 gagtcgtgac cggccccctc aacaaggagg caatggccct ggcggggttc aagtacgatg
     1681 gccaaacgga gctattcgcc gagcggaccg gcaccaagga atacgcgatg ctgttggtcg
     1741 tcgggcggat gcgggtcctc catgtctcga cccacacctc attacgaacc gtctgcgaca
     1801 aggtgaaaca ggccagaatc ctgaccgtca ttcgcctagc tcatcaggtt ctctgcgatc
     1861 tcggatcacg gaagaagcga atcggcgttg ccggcctcaa ccctcatgct ggcgagagtg
     1921 gtcggttcgg ccgggaggag atagaagaga tcatgccggc gatcgaggcc gcccgggccg
     1981 agggaatcag ggtaggcggc cccttcccac ctgacaccct gttccatcgg ctcaagctcg
     2041 gtgaattcga cgccgtcgtc gccatgtacc atgatcaggg ccatgtcccg ctcaagctga
     2101 tcgggttcca tcgaggggtc aatgtcaccg tagggctccc cattatccgc acctcagtgg
     2161 atcatggtac cgccttcgac atcgcgggga cagggaccgc caatcctcgc agcctggtgg
     2221 aggccatcct gctggccaca aagttcgccc atcgccggcg caagacaccc cgcgctctcg
     2281 acgtgtagtc ctggacggcg cccctgccta ctatactcag gcgatattga ttcgtcagtc
     2341 accgcggccc agggcgcctc ggtggcgtac ctgggtccga catcctcatc cccatgccac
     2401 ccatcatcga ttcgtgaccc aatcgatcga gtttctccat ttgctccggt gtcagcaccg
     2461 ccttcccggc ctcgatcgtc ttgaca
//
LOCUS       DATMXI010000130         4049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_76622, whole genome shotgun sequence.
ACCESSION   DATMXI010000130 DATMXI010000000
VERSION     DATMXI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76622"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..656
                     /locus_tag="VN444_02390"
     CDS             117..656
                     /locus_tag="VN444_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HWR20693.1"
                     /translation="MSRLTTERLSTLLEEYVRQTGVSVDLLLVGGLALQAYGYADRAT
                     QDVDGEVTGDLDPLVEFLRQHNIPADLGENMSGWSVIAMPPGYRDRTSVMREQPGLRV
                     RLLHPTDFVIAKLRRGTELDLEDAEYVVRRFDLTPEAVQAAAAAALMASPKDTALFLF
                     RKTVEIFCARLIGQPRDSS"
     gene            complement(849..1265)
                     /locus_tag="VN444_02395"
     CDS             complement(849..1265)
                     /locus_tag="VN444_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HWR20694.1"
                     /translation="MRAVADTNVYISALNFGGTPEEILGLGRARTIQLFISPSILEEI
                     EEVLLRKFYWSARNIREASRAIQEFAQLIHPREAIHLITEDEPDNRILECAVEANADC
                     VITDDRHLRQLETFRGISIVSSREFIGAYGARDLGG"
     gene            complement(1265..1504)
                     /locus_tag="VN444_02400"
     CDS             complement(1265..1504)
                     /locus_tag="VN444_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWR20695.1"
                     /translation="MRTSKILSLSLPPTLLQEAERLAKKEGRTKSELFREALRRYLQE
                     QRWAALRGYDAQQARKLGAKETDIERLIAEYRKGR"
     gene            1935..2960
                     /locus_tag="VN444_02405"
     CDS             1935..2960
                     /locus_tag="VN444_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20696.1"
                     /translation="MPTPKPAPSKAKKPKAEIKREFEEIVEEVTSAKEASTAKAEAIL
                     KQKEMDVREAVTDLTVEGVVQGITNLNLEISRFLAGLSDKLVREVNTLQSVREAITLE
                     QAEIERLHKIDVATTALDILVKEYQQQKEALEAEIGAARAAWDEQDRQREREQKEYED
                     ALKKQRQREVEEYEYKKTLERKKAQDKYEEELRLLEKKNREKQEALEKSWQSRDAELK
                     EKESAFARLSKEAEEFQGRLDREVERAVAEAVTSAGRSHEQQVVLLRKDVEVERRLAE
                     LQIRSLQETVLRQTTHIESLERRLEEAKGQVQDIAIKAIEGASGAKALSHVEQIAMEQ
                     AKTRVQP"
     gene            3166..>4049
                     /locus_tag="VN444_02410"
     CDS             3166..>4049
                     /locus_tag="VN444_02410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWR20697.1"
                     /translation="MSPMKVSLTWLSEYVDVAVPAKQLAHQLTMAGTEVGGIEERGNG
                     WPGIIVAQIESIDPHPTADSLTLVGLGLPGERMTVVSGATNLKVGDRVPFAPVGARLI
                     DAQTGRSTVVEVAQIQGVASRGVLCSEKELGLSEAHEGVLILPSEAEVGLSLNAVLGD
                     TILDLEVTPNRPDCLGMLGVAREVAAIMEAPLHLPDLTYQEEGSDVRDEVQVEILAPD
                     LCQRYSASVIHGVTVGPSPLWVQRRLTAAGVRPINNIVDITNYVMLEYGQPLHAFDYD
                     TIAGRRIAVRRATGGESLR"
BASE COUNT          887 a         1061 c         1261 g          840 t
ORIGIN      
        1 gtggtatacc acctaccgga gcctgatcca gaccttgctc ggccgttcgc ccctccctga
       61 tgatccaggc ccagtcgatc cccgcgagta ccgtctattt gcggaggcga tccgatttgt
      121 cgcgactcac gactgagcga ctttcaacgc tgctcgaaga gtacgtacgc cagaccggcg
      181 tgtctgtcga tctcttgctg gtcggtggtc tcgcactgca ggcatacggc tatgccgatc
      241 gtgcgactca ggacgtcgac ggcgaggtga cgggcgatct cgatcccttg gttgaatttc
      301 tccggcagca caatatccca gccgatctcg gagaaaatat gtccgggtgg tcggttatcg
      361 ccatgccgcc cggataccgt gatcggacct cggttatgcg tgagcagccc ggccttcgcg
      421 tgcggctctt acatccgacc gattttgtca tcgcaaagct acggcgagga acagagctcg
      481 atctggagga tgcggaatat gtcgtacgtc gcttcgacct tacccccgaa gcggttcagg
      541 cggctgcagc ggccgctctc atggcttcgc cgaaagatac agcgcttttc ctcttccgca
      601 agacagtcga gatattctgt gcgcgactga tcgggcagcc tagagattct tcgtagatct
      661 ccatcacctc actgcgctaa ccgcaaatca agctactcac catcttctcg gttccggttc
      721 ttaacagcct tcagcctatc tgcctgagta acaccacagc aaaaatactt gccagagggg
      781 gagccttatg agtgatagtc aacccgctgt ctttacagga tgaggcgctt ctccgtcttt
      841 gggctcactc aaccgccgag atctctggct ccgtaggctc ctatgaattc tctagagctt
      901 acgatagaga tgcctcgaaa cgtctctagt tgtcggagat gtcgatcgtc ggtaattaca
      961 caatcggcgt tagcctcgac ggcacactcc aggatacggt tgtccggttc atcctcggtg
     1021 atgaggtgga ttgcctcgcg aggatggatg agctgcgcaa actcctggat ggctctactg
     1081 gcctcgcgga tgttcctggc ggaccagtag aattttcgga gcagaacctc ctcgatctct
     1141 tcgaggatcg aaggggaaat gaagagctgg atggtcctcg ccctcccgag tcctaagatc
     1201 tcctcgggtg tgccgccgaa gttgagggca gagatgtaga cattggtgtc ggctaccgcc
     1261 cgcactaccg gcctttccga tactccgcaa tgagacgctc aatgtcagtc tctttagcgc
     1321 cgagcttacg cgcctgttgg gcgtcgtacc cgcgaagagc tgcccaccgc tgttcctgga
     1381 ggtaccgtcg gagcgcttcg cggaagagct cgctcttggt tctcccctcc ttctttgcga
     1441 ggcgctcagc ctcttggagc agggtaggtg ggagcgagag ggaaagaatc ttgcttgttc
     1501 gcatggctgc ctccttcata atacttgtta ttacttatgc tactcccagg tgagttgcat
     1561 gttaagcgag cgtattggta cggggagcgt gagcggggaa acgactgccg tgccatcctg
     1621 tgatgtgatg gagtagcgtg aactgaggtg ggtaatcaac tgggttcgct gaacccggcg
     1681 cagcactctg tgaagcaaag gggggtgaac catgtccatg cccgcgtgtc gaacgcgcct
     1741 cccatcgctg acgccattgc agaaaaaagg gagtcgccca gcctcaggag gaggagtccg
     1801 caccgtggag atggctctcc cctcgttggt tgacatttac cggccattga catagtctcc
     1861 ggcggtagcc gtgatgatcc gaatgtggaa tcgaaccgac tctgacattg cgaaatcgag
     1921 gagagggggt atacatgcca acgccgaaac cggcgcccag taaggcaaaa aagccaaagg
     1981 ccgagattaa acgggagttc gaggagatcg ttgaagaggt gacgtcggcc aaggaggcgt
     2041 cgaccgcgaa ggccgaggcg attctgaagc agaaagagat ggatgttcgc gaagccgtga
     2101 ctgatctcac agtggaaggg gtcgtccagg ggatcacgaa tctgaatctc gaaatctcca
     2161 gattcctcgc cggtctttcg gacaagctgg tgcgtgaggt gaatacgctc cagtcggttc
     2221 gggaggctat tacgctggag caggcggaga tcgaaagact gcacaagatc gacgtcgcta
     2281 cgacggcttt ggatatactg gtcaaggagt accagcagca gaaagaggcc ctggaagccg
     2341 agatcggcgc tgcgagggct gcctgggatg aacaggatcg gcaacgggaa cgggaacaga
     2401 aggaatatga ggatgccctc aagaagcagc gccagcgtga ggtcgaggaa tacgaataca
     2461 agaagacgct ggaacgcaag aaggctcagg acaaatacga agaggagctg cgattactcg
     2521 agaagaagaa ccgcgaaaaa caggaggcac tggagaaaag ctggcagtcg agagacgctg
     2581 agttaaagga gaaagagtcg gcatttgcgc gattatcgaa agaggccgaa gagttccagg
     2641 gtcggctcga tcgagaagtt gaacgggctg ttgcggaggc ggtgacatcg gccgggcgaa
     2701 gccatgagca acaggtggta cttctgagaa aggatgtgga ggtcgagcga cgtctggccg
     2761 aattgcagat acgatccctc caggagaccg tattacgcca gaccacacat atcgagtcgc
     2821 tcgagaggcg gctggaagaa gcgaaaggcc aggttcaaga tattgctatc aaggcgatag
     2881 agggagcttc aggcgccaag gccttgtcgc atgttgagca gattgctatg gagcaggcca
     2941 aaacacgggt gcaaccgtaa ggttggagag gactgctttg acgttgacac cagcagtttt
     3001 ttcgcgctat cgtccttgca cgaaaacccc ctcaatcccc ctttacgaaa gggggaggta
     3061 aggaaacgca tcagcccccc ctttttttga aaggggggat cagaggggtt tgtctgaagc
     3121 tgacggctga acgctgattg ctgaccgcta tttctgagga atctgatgag cccgatgaaa
     3181 gtgtcgctca catggctgtc tgaatatgtc gatgtcgcgg taccagccaa gcaactggcc
     3241 catcagttga ccatggcggg aaccgaggta ggcgggatcg aagagcgcgg caacggctgg
     3301 cctgggatta tcgtcgcgca gatcgaatct attgaccctc acccaacggc tgactctctc
     3361 accttggtgg gcttagggtt gcctggcgag aggatgacgg ttgtgagcgg cgccacgaat
     3421 ctcaaggtcg gcgatagggt gccgtttgcg ccggttgggg cgcgcctgat agacgctcag
     3481 acaggtcgat ccaccgtggt ggaggtggcc cagattcagg gggtggcctc gcgaggcgtc
     3541 ctctgctccg agaaggagct gggtctgtcg gaggctcacg agggagttct gatccttccc
     3601 agtgaggccg aggttggcct gtcgctcaat gcggtgctag gggacaccat tctcgacctc
     3661 gaggtcacgc cgaatagacc agattgtctg gggatgcttg gggttgcgcg cgaggtggct
     3721 gccattatgg aggcgccact tcatctgcct gatctgacct accaggagga aggttcagac
     3781 gtccgggatg aggtgcaggt cgagatcctg gcgcccgacc tttgccagcg gtatagcgca
     3841 tccgtcattc atggggtcac ggttggtccc tcgcccttat gggtccagag acgccttacg
     3901 gcagccggcg tgcgcccaat caataatatc gtggatatta ccaactatgt catgctcgag
     3961 tacgggcagc cgctgcatgc ctttgactac gacacgattg cgggcaggcg gatcgcggtg
     4021 cggcgcgcga caggtggaga atcgcttcg
//
LOCUS       DATMXI010000131         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_322646, whole genome shotgun sequence.
ACCESSION   DATMXI010000131 DATMXI010000000
VERSION     DATMXI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322646"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VN444_02415"
     CDS             <1..294
                     /locus_tag="VN444_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HWR20698.1"
                     /translation="VCVVMASRGYPGPYDQGALISGLEEAAAERGVMIFHAGTSRTKG
                     QILTGGGRVLGVTGLGRDIQGAIATTYRAVKKIHWEGAHYRTDIGARALARTS"
     gene            372..878
                     /gene="purE"
                     /locus_tag="VN444_02420"
     CDS             372..878
                     /gene="purE"
                     /locus_tag="VN444_02420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWR20699.1"
                     /translation="MARQPVVGIVMGSDSDLAVMELTAKALERFGIPFELKISSAHRS
                     LDATLRYIRQAETRGIKVIIAGAGAAAHLAGVIAGQTTLPVIGVPLASSSLKGLDALL
                     SVVQMPGGVPVATMAIGEAGARNAGILAARILALSDEALQRTLQSFKEALASEVEEKD
                     RTLQKRRS"
     gene            919..2475
                     /locus_tag="VN444_02425"
     CDS             919..2475
                     /locus_tag="VN444_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWR20700.1"
                     /translation="MNLAYPASGRQASLLFLFFLGLVTTFHLWFINSGHFNLAPDEAH
                     YWVWSKRLDWSYYSKGPMVAYLIALSTRLGGDTEFFIRLPAVLLSTGTALLTFLLAVR
                     LCRSTIAGLEAVLLLAAIPLYEAGSILMTIDAPLVFFWSLTLLLIHRALTANGNVWWI
                     FAGISLGLGLLSKYTMAVIVPQTFLYLALSRDHRFWMRRSGPYLALGVGLILFSPVIY
                     WNATHGLVSFRHLLEQLGGGKDTVLPLKNLGEFVVSQVGVVTPVLFVVLMIGLWEVGR
                     AGLTRCGDDASLFLFCASVPLLLACVITGLWTKVQANWAAPAFIAMAIAAAKWRSGAP
                     LQGYSAVRWTRSRALFAGALLTGFLVSAIGHFPRALASVGLPLPRKLDLTRRLRGWAD
                     LGAQVSAVYQEMSRNRSTFVFSDRYQIASEAMFYVPAHPTTFNIQLGRRMNQFDVWGG
                     TEEVRGWDAIFVTDRSDLPDAVLRSFDIVKLERPAHPLQVDHTRDLDSWSIFRCYGFR
                     GFPPAQQLGY"
     gene            2476..>2862
                     /locus_tag="VN444_02430"
     CDS             2476..>2862
                     /locus_tag="VN444_02430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20701.1"
                     /translation="MSDASPLCASNHDLQRAPWLSIIIPFCNEEENIRPLHDQIIAAM
                     VGLGQPYEVVAVDDGSTDRTLATLEAIVKDDSQWSVVVLRRNFGQTAALSAGFDHAKG
                     DVIVTLDGDLQNDPADIPRLLKSIQDY"
BASE COUNT          527 a          871 c          844 g          620 t
ORIGIN      
        1 gtctgcgtgg tcatggcatc aagaggctat cccggcccgt acgaccaggg cgccctgatc
       61 tccgggcttg aggaagcggc agcagaacgt ggcgtcatga tcttccacgc cgggacaagc
      121 cgtacgaaag gtcagatcct caccggaggg gggcgggtcc tgggtgtcac cggcctcggg
      181 cgagatatcc agggggcaat cgccaccacc taccgggcgg tgaagaagat tcattgggag
      241 ggcgcgcact accggaccga catcggcgct cgcgccctcg ccaggacctc ataacagtac
      301 ggcaaggcag cccgtagtcg ggatagaaga agaatacgtg gaaggagcga cagttgacca
      361 ctcagaacag tatggcaagg cagcccgtag ttgggatcgt catgggcagc gactccgatc
      421 tggcagtcat ggaactcacc gccaaggcgc tggagcggtt cgggatcccg ttcgaactca
      481 aaatcagctc tgcccatcgt tcccttgacg ccacactacg ctatatccgt caagccgaga
      541 cgcgagggat caaggtgatc atcgccggcg ccggcgccgc ggctcacctc gcaggcgtca
      601 ttgcaggcca gaccacgctt ccggtcatcg gggttccact ggcctcgagc tcgctgaagg
      661 gtctggacgc gcttctctcc gtcgtccaga tgcccggggg ggttccggtt gccaccatgg
      721 ccatcggaga ggcaggggca aggaacgccg gcatcctggc cgcgcggatt ctagcgctgt
      781 cagatgaggc cctccagcgc acgctgcaat catttaagga ggccctggcc tccgaagtgg
      841 aggaaaaaga tcgcacgctc caaaagcgcc gctcctgaga tccccttacc acagacccct
      901 tactccaggg aatcgttcat gaatttggcg tacccggcga gtggccggca ggcgtcgctg
      961 ttgttcctct tcttccttgg tctcgtcacc acctttcacc tctggttcat caactcaggc
     1021 cacttcaatc tggcgccgga cgaggcgcac tattgggtct ggtcgaagcg actggactgg
     1081 agctactata gcaaggggcc gatggtggcc tacctcatcg ccctctccac ccggttaggg
     1141 ggcgacacag aattctttat ccggctccct gcggtcctac tttcaactgg aaccgccctg
     1201 ctcacgttcc tgttggcagt tcggctctgc cgctccacta ttgccggcct ggaagcggta
     1261 ctgctccttg ccgccatacc gctgtacgag gcggggtcga tcctgatgac gatcgatgcg
     1321 ccgctggtat tcttctggtc tctcacgctg ttgctgatcc accgcgctct cacggcgaac
     1381 ggcaacgttt ggtggatctt cgcaggaatc agcctcggcc ttggtttgct cagtaaatat
     1441 acgatggcag tcatagtacc gcagactttc ctctatcttg cattgtcacg ggaccatcgg
     1501 ttctggatgc ggcggtcagg tccctacctc gctcttgggg tggggctcat cctctttagc
     1561 cctgtcatat attggaatgc gacgcacggc ttggtctcgt ttcgccacct cctggagcag
     1621 cttggagggg gaaaagatac tgttctgccg ctgaagaatc tgggggaatt tgtagtatcc
     1681 caggtaggcg tggtgacccc cgtcctgttc gttgtgctca tgatcgggct ctgggaggtc
     1741 ggacgggccg gacttaccag gtgcggtgac gacgcctcac tatttctctt ctgtgcctcc
     1801 gtgcccctgc tgctcgcctg tgtcatcacc ggcctgtgga caaaggtgca ggcgaactgg
     1861 gccgcgccgg cctttatcgc tatggccata gctgccgcta agtggcgatc aggcgcacct
     1921 ctccaaggtt actctgccgt gcgatggacg cgtagtcgcg cccttttcgc gggcgcctta
     1981 ctgaccgggt tcctcgtcag cgccattggt cactttccgc gcgcacttgc ctcggtcggc
     2041 ctgccgcttc ctcgcaagct cgatctgacc cggcgcctca gaggatgggc agatctcggc
     2101 gcgcaagtga gtgccgtcta tcaagagatg agtcgaaata gatcgacatt cgtcttcagc
     2161 gatcggtatc agattgccag tgaggcgatg ttctatgtac cggcccatcc gaccaccttt
     2221 aatatccaac tcgggcggcg aatgaatcag tttgacgtat ggggaggaac cgaggaagtt
     2281 cgaggctggg acgccatctt tgtgacggat cggtctgacc tcccggatgc cgttctccgc
     2341 tccttcgaca tcgtgaaact agaacggcca gctcatccct tacaggttga ccatacgcgt
     2401 gaccttgact cgtggtcaat ctttcgctgc tatggtttcc gtggattccc tccggctcag
     2461 cagctcgggt actagatgag tgacgcctca cccctatgcg cttcgaatca cgatcttcag
     2521 cgggcgccgt ggttgtccat catcatcccc ttttgtaacg aagaagaaaa catccggccc
     2581 ctccacgacc agatcatcgc ggccatggtc ggtcttggac agccctatga ggtggtcgcc
     2641 gtggacgatg gcagcaccga tcggacactg gccacccttg aggcgatcgt gaaggacgat
     2701 tcgcaatgga gtgtggtggt gcttcgacga aacttcgggc agactgccgc cttatccgct
     2761 ggctttgatc acgccaaggg cgacgtgatc gtgacccttg atggggacct tcagaacgat
     2821 ccagccgata tcccgaggct gctgaagtcg atccaggact ac
//
LOCUS       DATMXI010000132         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_150722, whole genome shotgun sequence.
ACCESSION   DATMXI010000132 DATMXI010000000
VERSION     DATMXI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150722"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..988
                     /gene="ttcA"
                     /locus_tag="VN444_02435"
     CDS             116..988
                     /gene="ttcA"
                     /locus_tag="VN444_02435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWR20702.1"
                     /translation="MSPLANPSHTRPRLDEITEQLQTKLLRLVGDTIGQYGLVQDGDK
                     VMVCLSGGKDSYALLDLLMVLQRRAPIHFDLVAVNLDQKQPGFPAHVLPNYLSDIGIP
                     FHIEERDTYSIVKRLIPEGQTTCSLCSRLRRGILYRVATELGATKIALGHHRDDLVET
                     LFLNLLYTGKLKTMPPKLRSKDGRHVVIRPLSSVRESDLARYAELRAFPIIPCDLCGS
                     QENLKRKQVKEFLHEWEQRSPGCTDSMFAALSNVAPPLLLDRRLFDFIGLHASDQTKG
                     EDDAWLDQDHSSFV"
     gene            1053..1706
                     /gene="mtnB"
                     /locus_tag="VN444_02440"
     CDS             1053..1706
                     /gene="mtnB"
                     /locus_tag="VN444_02440"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HWR20703.1"
                     /translation="MPHYPDFKTFAEQLVQTARWTSRMGWAPATSTNYSVRLPADAAP
                     AYCAITSSGVDKTAIEVEHILSVDQHGNALDVDGLKPSAETPLHLLLYRTMGAGAVLH
                     THSLPAALLSYLARDEGRVLLSGWELLKGLSGIDTHEVDISLPVFPNSQDMQALSMLV
                     ERSLSSERSCYGFLLAGHGLYAWGKDIPEAKRHLEVFEYLLQCQREVMWHGYAANTR"
     gene            1681..2208
                     /locus_tag="VN444_02445"
     CDS             1681..2208
                     /locus_tag="VN444_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HWR20704.1"
                     /translation="MATLRIPDKNVTLQDRFAVRQFLDLRGIVNERWSADRDLPSSAT
                     NEQVLAAYDYLLKPLMKKYRYHTTDVIAVDGQTPNLEGIREKFLREHTHSEDEVRVFV
                     EGHGLFWFHTEGDDDEVFALLCEQGDLISVPANTKHWFDLGDPPKVRAIRIFTDQAGW
                     VPHYTNSGIDQRYRW"
     gene            2216..2905
                     /gene="mtnC"
                     /locus_tag="VN444_02450"
     CDS             2216..2905
                     /gene="mtnC"
                     /locus_tag="VN444_02450"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HWR20705.1"
                     /translation="MVRYILMDIEGTLISVAFVREVLFPFAKHRLAPFLKERRHDPTV
                     LQLAAACQDVMERETGTRPAYEELPAMLTGWIDQDRKMAGLKALQGLIWDEGYRQGAF
                     APQLYDDVLPVLTQWRTLGIRLGIYSSGSEQAQRLLFAYTNVGDVTSLFEHFFDTTVG
                     EKKTSSSYRKISDQIDLPPPDILFLSDAELELDAAASIGFRTAHIVRPGTDASTRHPI
                     HPDLTLLPPAE"
     gene            complement(2955..3518)
                     /gene="msrA"
                     /locus_tag="VN444_02455"
     CDS             complement(2955..3518)
                     /gene="msrA"
                     /locus_tag="VN444_02455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWR20706.1"
                     /translation="MDQTAPPVQKGFSIATVAGGCFWCLEAVYDQMKGVVAVESGYIG
                     GHVDHPTYEAVCSGRTGHAEAVRVMFDPTVVSYRELLEVFFVIHDPTTLNRQGHDVGT
                     QYRSAVFFHGPEQKQIAEEVIAGMTKEKLYANPIVTEITPAGTWYEAEPYHQEYFARN
                     PFQGYCTAVVGPKVVKFRKQFASKLKP"
BASE COUNT          824 a         1041 c          943 g          852 t
ORIGIN      
        1 gtacatacga atagtactga cccaattttc ctcctggatc gattctcatc cttctccatg
       61 ggacctccga ctaacgatcg acagacatac agttgggcgc gtagaatgcc aacccatgtc
      121 acctttggca aacccttcgc acacacgtcc cagactggac gaaatcaccg aacagctgca
      181 gacgaagcta ctccgtctcg tcggggatac catcggccag tatggtcttg ttcaggatgg
      241 cgacaaggtt atggtatgcc tttccggcgg gaaagacagt tatgctctgc ttgacctatt
      301 gatggtctta caacggcgcg ctccgattca cttcgacctt gttgcggtga acttggatca
      361 aaagcagcca ggttttcctg cccatgtgct cccaaactat ctgtcagata tcgggatacc
      421 atttcatatc gaagaacgag atacatactc aatcgtcaaa cgcctcatcc ccgagggaca
      481 gaccacttgt tcgctctgtt ccagactccg ccgcggcata ctctatcgcg tcgccacgga
      541 attgggcgcg accaagatcg cattgggtca ccaccgcgat gatcttgttg aaaccctgtt
      601 cctgaacttg ctctatacag gcaagctcaa gacgatgccc ccgaaactga ggtcgaaaga
      661 tggccggcat gtggtcattc gtccattgtc ctctgtgcgg gagagcgatc tcgcgcggta
      721 tgcggaactc cgtgcgtttc ccatcattcc ctgcgatctc tgtggctcac aggaaaacct
      781 caagcggaaa caggtgaagg agtttctgca cgaatgggaa caacggtcgc ctggctgcac
      841 ggacagtatg ttcgccgccc tgtcgaacgt ggcgcctccg cttcttctgg atcgacggct
      901 gttcgacttc atagggctgc atgcttctga ccagacgaag ggggaagatg acgcatggtt
      961 ggatcaagat cattcctcat tcgtatgagc cttaccattc tcccgaatgg gagtaagatc
     1021 agcggcatct actttagcgc aggttgtagg ctgtgccaca ctatcccgat ttcaaaacat
     1081 tcgctgaaca gctagtccag actgcccgat ggacgtctcg catgggctgg gctcctgcca
     1141 caagcacaaa ctatagcgtg cggttaccgg ccgatgctgc ccccgcctat tgcgcaatta
     1201 ccagttccgg ggtggacaaa accgcaatcg aggttgaaca cattctctcc gtcgatcagc
     1261 atggcaatgc tctcgatgtt gacggactga aaccttcggc tgaaacccct ctccatttac
     1321 ttctctatcg cacgatggga gccggagccg tacttcacac ccactcactc cctgcagcat
     1381 tgctctctta cctggcccgc gatgaaggcc gagttctcct ctccgggtgg gaattgctga
     1441 agggcttgag cggcatcgat actcatgaag tagacatctc cttgccggtc tttccgaact
     1501 cacaggatat gcaggcattg tcgatgcttg tggaacgatc tctgtcgagt gagagatcct
     1561 gctatggttt tttgctggca ggacacggcc tctatgcctg gggcaaggat ataccagagg
     1621 cgaaacgaca tctcgaagtc ttcgaatatc ttctccaatg ccaacgagag gtgatgtggc
     1681 atggctacgc tgcgaatacc cgataagaac gtgacgttgc aggatcgatt tgccgtccga
     1741 caatttctcg acctgcgagg catcgtcaat gaacggtggt ccgcggacag agacctacct
     1801 tccagcgcca cgaacgagca ggtcctcgcc gcctacgact acctactcaa acccttgatg
     1861 aaaaaataca ggtaccacac gacagatgtc attgcggtgg atgggcagac accgaacttg
     1921 gaagggattc gcgaaaaatt tctccgagaa cacactcaca gcgaggatga agtgcgtgtc
     1981 tttgttgaag gccacggcct cttttggttt catacagaag gggacgacga tgaagttttc
     2041 gccctgttgt gcgagcaagg tgatctgatt tctgttccgg ccaacacaaa acactggttc
     2101 gacttaggtg atcctccaaa ggtgcgggca attcggatct tcaccgacca ggccggctgg
     2161 gttccccact atacaaattc cggaatcgat cagcgctatc gctggtagcc gcctcatggt
     2221 ccgatacatc ctcatggaca tcgaaggcac tctcatttcc gtcgcctttg tccgcgaggt
     2281 cctctttccc tttgccaaac accgtctcgc gccctttctc aaagagcggc gccatgatcc
     2341 cactgtgcta cagttggctg cagcatgtca ggatgtcatg gagcgcgaaa caggaacgcg
     2401 ccccgcgtat gaggagctgc cagccatgct gaccgggtgg atcgaccagg accgaaaaat
     2461 ggccggcctc aaagcattgc agggactgat ctgggatgag ggctatcgac agggggcttt
     2521 tgcaccgcaa ctgtatgacg atgtcctgcc tgtcttaaca caatggcgca ctcttggtat
     2581 ccgcttggga atctactcct caggttcaga acaggctcag cggctcctct ttgcatatac
     2641 caacgtcggg gatgtgactt ccttgtttga gcactttttc gataccactg tcggcgagaa
     2701 aaagacctcg tcgagttacc gcaagatcag cgatcagatc gatctaccgc ctcctgacat
     2761 tctttttctg tcggacgcgg aactcgaact ggatgctgct gcatcgatag gcttcaggac
     2821 cgcccacatc gtgcggcctg gaaccgatgc gagtacccgc catcctattc atccagacct
     2881 cacgctcctt ccccctgcag aataggaatt cgggcgaacg aatcgtttgg gatacctcaa
     2941 tgaagtggga agggttacgg ctttaacttc gacgcgaatt gctttcggaa cttaaccact
     3001 ttcggcccga ccacagctgt acagtagccc tggaagggat tccgtgcaaa atattcctgg
     3061 tgatagggct cggcttcgta ccaggtcccg gcgggagtga tttccgttac gatcgggttg
     3121 gcatagagct tttccttcgt catgcctgcg atcacctcct cagcaatctg tttctgctca
     3181 ggtccatgga agaagactgc tgagcggtat tgagtcccga catcgtgccc ctgccgattg
     3241 agggtcgtag gatcgtggat gacaaagaac acctccagca actcgcggta gctgacgacg
     3301 gtgggatcga acatgacacg gactgcttct gcgtgaccag tcctgccgct acacacggct
     3361 tcatacgttg gatggtctac gtgaccacca atgtagccgg actcgaccgc caccacaccc
     3421 ttcatctgat catagaccgc ttcgaggcac caaaagcagc cgccggccac ggttgcaatg
     3481 ctgaatccct tctgtaccgg aggcgcagtc tgatccatcg tacagacttc tgattacgcg
     3541 gtctgaaaat gagtcttggg aggtctttct ttcagctcct gtcgagcctt ataccataaa
     3601 gaatcgggag gctgcgacca gttcgtgaac gaacagggag atcctcacgc gtcactgtag
//
LOCUS       DATMXI010000133         6898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_298896, whole genome shotgun sequence.
ACCESSION   DATMXI010000133 DATMXI010000000
VERSION     DATMXI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298896"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1226
                     /locus_tag="VN444_02460"
     CDS             282..1226
                     /locus_tag="VN444_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR20707.1"
                     /translation="MKSGQPIRVGVVGVGYVGQHHARIYSEMAGVELVGVADIDETRL
                     QELGARYPIQLCRDYREILGTVDAVSVAVPTLLHYSIAKEFLERGVDVLVEKPIAQRL
                     DQADELVGVAKADDRIFQVGHIERFNGAVKALEAVVRSPGFIECHRLGPFANRNTDVD
                     VVLDLMIHDIDIVLNLVKSPVVAITAVGVPVISDQVDIANARLQFESGCVANLTASRV
                     SVERVRRVRIFQRDTFISLDYAQQEIAVYHRIPAADPGAETTVTIMKEDIPIDKAEPL
                     RVEIESFIECVRTRKRPLVSGEDGREALKVASQIIEKL"
     gene            1232..2404
                     /gene="lpxB"
                     /locus_tag="VN444_02465"
     CDS             1232..2404
                     /gene="lpxB"
                     /locus_tag="VN444_02465"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWR20708.1"
                     /translation="MRDGRILIVAGEPSGDLHAACVVQELLRRAPHLTIEGIGGDRMR
                     QAGVRLHAHAGDLAVVGIVEVAARLPAIWRAYRSMIRCLRERRPDLMILVDFPDFNLR
                     LARRAFRLGIPVVYFISPQVWAWRAGRIRSIAKYVRRLLVIFPFEEGFYRERGVEAVY
                     VGYPLLDRLTSLPSMEEARRRLELEGAAPVLGLLPGSRVGEIMRHLPILLRSIRHLMV
                     ERPGLRVIIAVADGLPLDLIESCLNQEAISARVVQGRTYEVMAASDLILVASGTATLE
                     AAIIGTPMVIVYRLAFLSWLLGRVLIRVPYVGMVNLVAGRRVVPELIQFHATPERIAD
                     EARRLLLSTEQRRRMRQELQQVRDRLGPPGAVDRTVDAILECLQPGAGEGMAVQRG"
     gene            2404..3099
                     /locus_tag="VN444_02470"
     CDS             2404..3099
                     /locus_tag="VN444_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWR20709.1"
                     /translation="MEKSPFSRAVAALREDRLVLRIVPWLAARFMQCLFRLLCVVHVG
                     RTYPESCWARGERIIVVFWHGRLLMMPFVYPGKPGALLVSQHRDGEYFSRIATILGFE
                     VIRGSATRGGMRALKQMIRVIKERLNLVVTPDGPKGPRAKVRPGVIEVAKLTGAPIVP
                     VSFSASRRRFLKSWDAFLVPVPFSRAVYIWGEPMYVPPTATRDEVTNYQETLEERLNL
                     LTMKADDYFRADL"
     gene            3187..4473
                     /locus_tag="VN444_02475"
     CDS             3187..4473
                     /locus_tag="VN444_02475"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWR20710.1"
                     /translation="MYFTYSLLLTLVLLAWSPSVILRALRSPYYRGGWRERLGYYPKP
                     LLSRLQAVQPVWIHAVSVGEVGVASILANLWTARRPTLPLLVSTVTGTGREVARRTFP
                     QTAAVVYFPIDLPMIVHRTLATVRPRLILLTETEIWPNFLHECAASKIPVAMINGRIS
                     ERSFSRYRLVRPFIRQVLQCVDLFCMQTGTDAKRILALGASPERVNVVGNLKFDAAPH
                     ADASSLAEQWRRELHIEAERPILVAGSTHAGEEEVLLQVFGRLRGEFPDLLLILAPRH
                     PERVAQVETVVAGHGLAAVRRSASAQGRDGARDVILMDTVGELSALYAVGSIAFVGGS
                     LIPRGGHNLLEPALHGRPVLFGPHMENFIEASAYFIERGAAIQVSDAADLTRQIARLL
                     RDPAARERMGQAAMAALAVHQGACERTVALLEQLVL"
     gene            4470..5594
                     /gene="lpxK"
                     /locus_tag="VN444_02480"
     CDS             4470..5594
                     /gene="lpxK"
                     /locus_tag="VN444_02480"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWR20711.1"
                     /translation="MKGIEALSAWTQHCMAEASEQWTAQAWLTCLRPASYAYGAINSL
                     RAALFASGLARTHRLPVPVLSVGNVSAGGSGKTPFVEMLAARLREQGQQVVIISRGYR
                     GGSKKPTIVSDGRDLRCEPPVAADEAYLLARHLPGVAVLTGANRYRVGEVALEHVDCD
                     VIILDDGFQHLRLYRDLDIVLVDAVNPLGYGRLLPSGLLRESPEAFGRADLVVITHAD
                     AGRNLDLAIQAIRRHAPITPIALAVHRPVNLIDVRSGEYLDFDRLTGQRLLAVSGIAN
                     PSRFEATLVQLGAFVAAHRIFPDHHRYSIADLEIINGAAMDSGASMVVITEKDMVKLT
                     CLDLAKVGAPLYALSISLELVEGAEVLDAMLSRLVKPSPS"
     gene            5591..6574
                     /locus_tag="VN444_02485"
     CDS             5591..6574
                     /locus_tag="VN444_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWR20712.1"
                     /translation="MKSKTMGGIAAYLEYLLAMGLAKGLLYLPSSVAYYAGEGLAGLL
                     YRFDHKHRLIAHENLHRAFHGELSSMRIAELTRLCFINLGRTVVETCRSLKIDRENFR
                     QFIRVEGYEHIQQAKRRGKGIIYITAHLGSWELLPLAVGLMGEPMSAVTRPLDNPYLD
                     RAINRLRTAWGTRVLAKKQVMPVLVRALLRGESVAILMDQNTTWKEGVFVDFFGMQAC
                     TALAPALLALRTDASVLPAAIIRCGRDRHTVIVEKEIPLIRTGRLKVDVVANTASFTK
                     AIEAFIRREPDQWLWVHRRWKTQPRATCQTPSLESGTWHVEPGTARQNPVQ"
     gene            6571..>6898
                     /locus_tag="VN444_02490"
     CDS             6571..>6898
                     /locus_tag="VN444_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20713.1"
                     /translation="MSGTTLRVKRTFEPDRLRSILAVSPNWLGDAVLALPALANLRRT
                     FPDASISLLVRPWLSQLFRSLPFIDELVELPRRGELLWAATALRKRNFELALLLPNSF
                     RTALISR"
BASE COUNT         1302 a         1907 c         2162 g         1527 t
ORIGIN      
        1 actgtggtgg ccctcgaaga tgcgggcgcg actgcgcttg ccctggatgc cgatcagacc
       61 ttgattctcg atcgggagaa ggtggtggcg actgccgacc ggcttgggtt ggcgattgtg
      121 gctgattagc agcaggacgg cggtcagccg tcagcgcagg ggcaggactt gccctgcccg
      181 aatagggcgc agcaagcagc gcccctacgt ctctgaatgc tgatcgctgc cgttaagagc
      241 gagttcgccc ataccgatgc aagaggaagg aaaaattggg aatgaagagc gggcagccaa
      301 ttcgagtcgg tgtagtgggt gtcggctatg tggggcagca ccacgccagg atctattcgg
      361 agatggcggg cgttgagctg gtcggggtgg ccgatatcga cgagacccgc ctgcaggagt
      421 tgggggcgcg gtacccgatt caactgtgtc gcgattaccg cgagatcctg gggacggtag
      481 atgccgtcag cgtggcggtg ccgaccctgc tgcactattc gatcgctaag gagttcctgg
      541 agcgcggggt ggacgtcctg gtagagaaac cgatcgcgca gcgcctcgat caggccgacg
      601 aactggttgg ggttgccaag gctgacgatc gaatcttcca ggttggtcat atcgagcgat
      661 tcaatggagc agtaaaggcg ctcgaggccg tcgtgaggag tcctggtttt attgaatgcc
      721 accgtcttgg ccctttcgcc aaccgcaaca cggatgttga tgtcgtcctc gacctgatga
      781 tccacgatat tgacatcgtg ctaaacctgg tcaagtcgcc tgtcgttgca ataacggctg
      841 tcggtgttcc ggtcatctcc gatcaggtgg acatcgcgaa tgcacggctc caatttgagt
      901 cgggatgtgt cgcgaacctg accgccagcc gggtgagcgt tgaacgagtt cgacgggtgc
      961 ggatatttca gcgggacacc ttcatctctc ttgactatgc tcagcaagag atcgctgtct
     1021 accatcgcat tccggcggcg gatccgggtg ccgaaacaac cgtcacgatt atgaaggaag
     1081 atatccctat tgataaagct gagccgcttc gcgtcgagat cgagagcttt attgagtgcg
     1141 tccggactag gaagagaccg ctggtctcag gcgaggacgg ccgggaggcc ctgaaggtgg
     1201 cctctcagat tatagagaaa ctgtaggcca gatgcgagat ggccggattc tgatcgtcgc
     1261 aggggagccc tcaggagacc tgcatgccgc ttgcgtagtt caagaacttc tgcggcgcgc
     1321 cccccacctg actatagagg gaattggagg agaccggatg cgccaggcgg gtgtccgtct
     1381 ccatgcgcat gcgggcgatc tggcggtggt cggcatcgtc gaggttgctg ccaggcttcc
     1441 tgccatctgg cgggcatacc ggagcatgat ccggtgttta cgcgaacgac gcccagatct
     1501 gatgatcctt gtggattttc cggacttcaa cctgaggctc gcccgccggg catttcggct
     1561 tggcatcccc gttgtctact tcatcagtcc gcaggtgtgg gcctggcgtg ccggccggat
     1621 acggtcgatc gcgaagtatg tccgacggct cctggtgatc tttccctttg aggagggttt
     1681 ctatcgcgaa aggggggtcg aggccgtgta tgtcggttac ccactcctgg atcggttaac
     1741 gtctttaccg tcaatggagg aggcgagacg ccgcctggaa ctggaggggg cggccccggt
     1801 gttggggctc ttgcccggca gccgcgtggg tgagatcatg cgacatctgc ccatcttgct
     1861 cagatcgatc agacatctga tggttgagcg gccgggtctt cgcgtcatca ttgccgtagc
     1921 cgacggtctt cctctcgacc tcatcgaatc ctgcttgaat caagaggcta tctcggcgag
     1981 agttgtacag gggcgaacct atgaggtcat ggctgcgtcg gacctgatcc ttgtggcttc
     2041 aggtaccgcg accctggagg cagccatcat cggtacccct atggtgatcg tgtatcgcct
     2101 cgcatttctt tcgtggctac tgggccgcgt gttgatcagg gttccgtacg tcggcatggt
     2161 aaatctggtg gcgggaagac gagttgtgcc cgagctgatt caatttcacg cgacgccgga
     2221 gcggatcgct gacgaagcgc gccgattgct cctatcgaca gagcaacgtc gccggatgcg
     2281 gcaggagctc cagcaggtgc gtgatcgatt ggggcctcct ggagccgtgg accggacggt
     2341 agacgctatc cttgagtgct tgcagcctgg cgccggggag gggatggcgg tccagagagg
     2401 gtgatggaga agtcgccctt ttcccgggcg gttgctgcgc tcagggaaga tcgccttgtt
     2461 ctgcgtattg tcccatggct tgctgcgagg ttcatgcaat gcctatttcg gctgctttgt
     2521 gttgttcatg tgggccgtac gtacccggaa agctgctggg caaggggaga gcgaattatc
     2581 gtggtcttct ggcacggccg gctcctgatg atgccctttg tctatcccgg gaagcctgga
     2641 gcgctcctcg tcagccagca tcgggatggg gagtatttca gccgaattgc aacgattctc
     2701 gggttcgagg tgattcgagg ctcagcgacg cgaggcggca tgcgcgctct caagcagatg
     2761 attcgcgtca tcaaagagag gctgaacctg gttgttaccc cggatggccc aaaaggaccg
     2821 cgagcaaagg tcaggcccgg tgtcattgag gtggcgaagc tgacgggggc tcctattgtg
     2881 ccggtgagct ttagtgcgtc gcgacgccga ttcctgaaga gttgggacgc cttcctcgtg
     2941 ccggtcccgt tctcccgtgc cgtatacatc tggggggaac cgatgtatgt tcctccaacg
     3001 gcgaccaggg atgaggtgac aaattatcag gaaaccctcg aagaacgcct caacctgctc
     3061 acgatgaagg ccgacgatta cttcagggcg gatctgtgac gaccgataaa tatggcgtct
     3121 gtggcgttta ttgcgtctaa gacattgcta caacaacgca atccacgtcg cacgcaatga
     3181 acgcacatgt attttaccta ctccctcctg ctgaccctcg ttttacttgc ctggtcaccc
     3241 tctgtcattt tgagggccct tcgaagcccc tactaccggg ggggatggcg tgagcgtctg
     3301 ggttattacc ctaaaccgct actctcacgg cttcaagccg ttcagcctgt ctggatacat
     3361 gccgtctcag tgggggaggt cggtgttgcc tccatcctgg ctaatctctg gacagctcgt
     3421 cggccaacgc taccgcttct ggtctcaaca gtaaccggaa ccggacgaga ggttgccaga
     3481 cgaacgtttc cgcagacagc ggcagtggtg tacttcccga tcgatctccc catgatcgtt
     3541 catcggaccc tggctaccgt gagaccccgc ctcatccttc tcacggagac ggaaatctgg
     3601 ccaaattttc tccacgaatg tgctgcctca aagattccgg ttgccatgat caatggtcgg
     3661 atatcggagc gctcgttttc acgctaccgc ctcgtccgac ccttcatccg tcaggtcctt
     3721 cagtgtgttg atctcttttg tatgcaaacc ggcacagacg ccaagcggat tcttgcgcta
     3781 ggcgccagcc ctgagcgggt gaatgtggtg ggcaatctca agttcgatgc agccccccac
     3841 gctgatgcct catcgcttgc cgaacagtgg cgtcgggagc ttcacatcga agccgagcga
     3901 ccgatcctgg tggcaggaag cactcatgca ggcgaggaag aggtgttgct ccaggtcttt
     3961 ggccgcttgc gcggtgagtt cccggacctt ctgctgattc tggcgccacg ccatcccgag
     4021 cgcgtagccc aggtagagac ggtagttgcc ggtcatggcc tggccgcagt gaggcggagc
     4081 gcctcggctc aaggaagaga tggcgcaagg gatgtgatcc tcatggacac cgtcggtgag
     4141 ctgtcggcgc tgtatgcggt ggggagtatt gcctttgtcg gagggagcct catacccaga
     4201 ggtggccaca acctgcttga gccggcccta cacggtcgtc cggtgctgtt cggtccccat
     4261 atggagaatt tcattgaagc gagcgcgtac tttatcgagc ggggggcggc tatccaggtg
     4321 agcgatgcag cggaccttac ccggcagatt gccagactcc tgcgtgatcc cgccgcaagg
     4381 gagaggatgg ggcaagccgc aatggcagcc ttggcggtcc atcagggcgc gtgcgagcgg
     4441 acagtggccc tcctggagca gctcgttttg tgaaaggcat agaggcgctc tcagcctgga
     4501 cgcaacactg catggcggag gcttcggagc agtggaccgc tcaagcctgg ctgacgtgct
     4561 tgcgtccggc ttcgtatgcg tatggggcaa taaactcgct tcgagctgcg ctctttgcgt
     4621 ccggcctcgc caggactcac cgacttccag ttccggtact gagcgttgga aacgtcagtg
     4681 ccggcggaag cgggaagacc ccgttcgttg agatgttggc tgctcggctg cgcgagcaag
     4741 gccagcaggt ggttatcatt tcacgggggt atcgaggtgg ttcgaagaag ccaacgattg
     4801 tcagcgatgg cagagattta cgatgtgaac cgccggtagc ggctgatgag gcctatctgc
     4861 tggccaggca tctgccgggc gtcgctgtct tgaccggagc caaccgatac cgggttggtg
     4921 aggttgccct tgagcatgtg gattgcgacg tcatcattct ggatgatggg ttccagcatc
     4981 tgaggcttta ccgcgacctg gatatcgtcc ttgttgacgc ggtcaatcct cttggatacg
     5041 gtcggctgtt gccgtcgggg ttgttgcggg agtcgccgga ggcgttcgga cgggccgacc
     5101 tcgtggtcat cacccacgct gacgccggac gcaacctgga cctcgccata caggcgatca
     5161 gaaggcatgc gccgatcacc ccgatcgctc tggctgtcca tcgtcccgtc aacctcatcg
     5221 acgtgaggag tggagaatac ctggattttg atcgtctcac tggccagcgg ttgttggcag
     5281 tctctggcat tgccaatccg agtcggtttg aggctaccct tgttcagctt ggggccttcg
     5341 tcgcggctca ccgcatcttt cccgatcacc accgctattc tatcgccgat ctggagatca
     5401 ttaacggagc ggccatggat agcggtgcct ccatggtagt catcacagaa aaggatatgg
     5461 taaaattgac gtgcttggac cttgcaaagg tgggggcacc gctatacgcc ctgtcgatct
     5521 cactcgaatt ggtggaaggg gcggaggtat tagatgccat gttgagccgc ctggtgaaac
     5581 cgagcccctc atgaagtcca agaccatggg cggtattgcc gcctatctcg aatacctcct
     5641 cgcgatggga ctcgcgaagg gattgttgta cctgccatct tcagtggcct actatgcagg
     5701 tgaggggttg gcggggcttc tctaccgctt tgatcacaag catcgtctca ttgctcatga
     5761 gaacctgcat cgggcctttc atggtgagct ttcctctatg aggattgctg aactgacgcg
     5821 cttatgcttt atcaatctcg gccgaaccgt tgttgagacc tgtcggagtc tcaagatcga
     5881 tcgagagaac tttcggcaat tcatccgggt cgaagggtat gagcacatcc agcaggccaa
     5941 gcgcagaggg aaaggaatca tctacatcac cgcgcacttg ggttcgtggg aactgcttcc
     6001 gcttgcggta ggccttatgg gcgagccgat gtctgctgtg acgcgccctc tggataatcc
     6061 atacctcgat agggcaatca accggttgcg gaccgcctgg ggaacgaggg tgttagccaa
     6121 gaaacaggtg atgccggtgc tggtgcgggc gctgcttcgt ggggagagcg tcgcgatcct
     6181 catggatcaa aacaccacct ggaaagaggg ggtctttgtc gatttctttg gtatgcaggc
     6241 ctgcacggct cttgcgccgg ctctcctggc cctcagaacc gacgcatcgg ttcttcctgc
     6301 cgccatcata cggtgcgggc gggaccggca cactgttatt gtggagaaag agatcccctt
     6361 aatcaggacc gggcgcctga aggtggacgt cgttgccaac accgcctctt tcaccaaggc
     6421 catcgaagcg ttcatccggc gggagccgga ccagtggctc tgggtccatc gcagatggaa
     6481 aacgcaacca cgtgccacgt gccagactcc aagcctagaa tccggaacct ggcacgtgga
     6541 acctggaact gcgaggcaga atccggtaca atgagtggga cgacgcttcg cgtaaagcga
     6601 accttcgaac cggatcggct tcgatccatc ctggccgtaa gccctaattg gttgggtgac
     6661 gcagtcctcg ctctacctgc gctcgccaac cttcgccgaa cctttcctga tgcaagcatc
     6721 tcactcctcg tacgcccgtg gcttagccag ttgtttcgat ccttaccatt catcgacgaa
     6781 ctcgtcgagt taccacggcg gggtgaactg ctatgggcgg cgacagcact gcggaaacga
     6841 aactttgaat tggctctgct cttaccgaac agcttccgga cggcgcttat cagtaggc
//
LOCUS       DATMXI010000134         3081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_290822, whole genome shotgun sequence.
ACCESSION   DATMXI010000134 DATMXI010000000
VERSION     DATMXI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290822"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VN444_02495"
     CDS             complement(<1..391)
                     /locus_tag="VN444_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HWR20714.1"
                     /translation="MSEILTQEEIDSLLSAAAKGAVPAAGAPTQVKRQFIRYDFRRPN
                     RISKEQLQALQMLHDRFAKQMGGTLSPLLRTFVEIRPTLVEQMAYAEYIASVAYPACL
                     GIFGMKPLKGGAIVELPPRLVFYIIDRI"
     gene            complement(761..1312)
                     /locus_tag="VN444_02500"
     CDS             complement(761..1312)
                     /locus_tag="VN444_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HWR20715.1"
                     /translation="MADDAKAEIREVGGSESLGGEEKKASAAGSGKLKAIAIQAGIVM
                     ALTGAAFGINVYVLKPMLVVNGKEQAKESAAKPKAEHHAGKILPLDPAIVNIAGTNGR
                     RYLKVTVQIQIPEEEKLVKEVEARKALLSDRLIQILSGKPLEEVTSTGSLQALKQQIA
                     DQFGKELGADRLQDVYLTEFVIQ"
     gene            complement(1337..1528)
                     /locus_tag="VN444_02505"
     CDS             complement(1337..1528)
                     /locus_tag="VN444_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HWR20716.1"
                     /translation="MVKLSRINGAEVTVNAELIEIIEATPDTIVSLTTGKKLMVVESV
                     DQVVEKVMAYRRVIATPLR"
     gene            complement(1559..2719)
                     /locus_tag="VN444_02510"
     CDS             complement(1559..2719)
                     /locus_tag="VN444_02510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWR20717.1"
                     /translation="MPLINVRREIPPQGLRILYLLADQTGCGWYRCLLPGIYLRNLYG
                     IDTRAATSLTDEVRAFARDADILVFQRQLSDTALDFLHAQKAMGKKIVYELDDDFWHL
                     PVKNPVYRYYQADGLRRLAGFIRVADVVTVSTEPLKRVVDTMHDDVVILPNAVDPDLA
                     DTIVGSRLGRQTCRHAMVRIGWSGSNFHEGDLDCVIDALITLTKRPDVQLVFFGWVPE
                     RIQREVPIGRLEIHPFVPTNSYYHAMAALRLDIGLAPLRDNRFNEAKSNLKYLEYSMF
                     KVPTVASPIYPYACTITDGEDGILVKKNRHQEWLRQLTRLVEDRAERDRLARNAYATV
                     RERFHLKSHVRQWLDLYRGLRCGSDGTAISTSSHGLSSMDRSRDEAEVAAVC"
     gene            complement(2797..>3081)
                     /locus_tag="VN444_02515"
     CDS             complement(2797..>3081)
                     /locus_tag="VN444_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWR20718.1"
                     /translation="VIDTLKLASFSNPAGLLRLANGQFGETPNSGVAQFGAAGTGGRG
                     TIVAGALEQSNVDLGTELVNLIIAQRGYEANAQVISVANQIQQTTLNLLR"
BASE COUNT          673 a          909 c          812 g          687 t
ORIGIN      
        1 ggatccgatc gataatataa aagacaagcc gcggcggaag ttcgacaata gcgccgccct
       61 tcagcggctt catcccgaag atacccaggc aggcgggata ggcgaccgat gcgatatatt
      121 cggcatacgc catctgctct accagggtcg gccggatctc aacaaatgtt ctcaggagcg
      181 gcgagagggt acctcccatc tgtttcgcga agcggtcgtg aagcatctga agggcttgga
      241 gctgttcctt ggagatccta ttgggcctgc gaaaatcgta acggatgaat tgtcgcttga
      301 cctgggtcgg cgcgccagcc gccggtacgg cgccctttgc ggccgccgac aacagcgagt
      361 cgatctcttc ctgtgtcaga atctcactca tcaaatgatc tcgctcggtg gttaatgatc
      421 ggtgatcggt tgtcgctgat cgttggaagc tgaccgcgac tcatggaggt atagcaagtg
      481 taatgccagc agaagcagaa cacttttgta acgattggag cgattttcaa ttagatgaga
      541 agagcggata cggccccgcg cctctcggcg tgaccaatac ctcaccacct gattcgtagc
      601 gacctgggaa accgcgtgat cataagcaga tagcagagat gatgaaattt gatcggatgg
      661 aaaaaaatta gccgtgcggg tacaagggat gtaatggacg agtagaaagt tcgcggtgtg
      721 aggcacaaaa tatagggtgg gcgtagggta tggatgaagc ctactggatt acgaactcgg
      781 taagatagac atcctgcagt cgatcggccc ccagttcctt accgaactga tccgcgatct
      841 gctgcttcag ggcctgcaga cttcccgtac tcgtcacctc ttccagcggc ttaccggaaa
      901 gaatctggat caaccgatcg cttaagaggg ctttgcgtgc ctctacctct ttaaccagtt
      961 tctcttcttc cggaatctgg atctgaacgg tgactttcag atagcgacgt ccatttgttc
     1021 cggcaatgtt gacaatagcg ggatcgagag ggagaatttt cccggcgtga tgctctgcct
     1081 tcggcttagc ggccgactcc ttcgcctgct ccttcccatt taccactagc atcggtttca
     1141 acacatagac gttgatacca aaggcagcgc cggtcaatgc catgacgatt cccgcctgaa
     1201 tggcgatcgc cttgagcttc ccgctacccg cagccgaggc tttcttctcc tccccgccaa
     1261 gcgattccga acctccgacc tcacggatct cagcttttgc gtcgtcagcc acctgtcgcc
     1321 tcccgcactc ccctcatcaa cggaggggcg ttgcgatcac acgtcgatag gccatgacct
     1381 tctcaaccac ctgatcgacg ctctccacaa ccatcagttt cttccccgtg gtgagtgaga
     1441 cgatcgtatc gggggttgcc tcgatgatct cgatcagctc cgcattcacc gttacctcag
     1501 cgccgtttat tctgctcagc tttaccatca tcagttcgag tgtccatccg tgtcaatctc
     1561 aacagacagc cgccacctca gcttcgtcac gcgagcgatc catcgacgac aacccatggg
     1621 acgacgtcga gatcgccgtt ccgtctgacc cacagcggag acccctgtaa agatccaacc
     1681 actgtcggac atggctcttc agatgaaatc tttctcttac cgtcgcatag gcgttgcgag
     1741 ccagtcgatc ccgttccgct ctgtcctcta ccagcctcgt caactgtctg agccactcct
     1801 gatggcgatt cttttttacc aggatgccgt cctctccatc agtgatagtg caagcatacg
     1861 gatagatggg agaggccaca gtcggcacct tgaacatact gtactcgaga tatttcagat
     1921 tgctcttcgc ctcattgaaa cggttatcgc gcagcggcgc caagccgatg tccagccgga
     1981 gagccgccat ggcatggtag tagctgttgg tcggcacgaa cggatgaatc tcgagtctgc
     2041 ctatcggcac ctctcgctga atccgctcag gtacccagcc gaagaagacc aattgtacgt
     2101 cgggacgctt cgtcaaggtg ataagggcgt cgattacgca gtcgagatcg ccctcgtgaa
     2161 aattcgatcc ggaccagccg atcctcacca tcgcatgacg acaggtctgc cgccccaagc
     2221 ggcttccaac aatcgtatca gcgagatcgg gatcgaccgc gttgggcagt atgacgacat
     2281 cgtcgtgcat cgtgtctacc actcgcttga gcggctccgt cgagaccgtc acaacgtcag
     2341 ccacgcggat aaatccggca agccttctaa gcccatcggc ctgatagtat cggtagaccg
     2401 ggttcttcac cggcaagtgc cagaagtcat cgtcgagttc atagacgatt ttcttaccca
     2461 tcgccttctg cgcatgcagg aagtccaggg cagtgtcaga aagctgccgt tgaaatacta
     2521 ggatgtcggc gtcccgcgca aaggctctca cctcgtcggt taaggaggta gcagccctcg
     2581 tatcgattcc gtagaggttg cgcaggtaga tcccgggcag cagacaccgg taccacccgc
     2641 acccggtctg atcggccagc aggtagagga ttctaaggcc ctgcggcggt atctctcttc
     2701 ttacgttgat cagcggcatc acatacaccc agcaagaagc tttcagcggt cagccgtcag
     2761 cttttgctga tcgctgaaag ctgaccgctt gattcatcat cgcaacaagt tgagggtggt
     2821 ctgttgaatc tgattagcca ccgagatcac ctgcgcattc gcctcgtacc cgcgctgggc
     2881 aatgataagg ttaaccaact cggtgcccag atcgacattc gattgctcaa gggcccccgc
     2941 gacgatcgta cccctgccac cggtgccggc agcgccaaat tgggccacgc ctgagttcgg
     3001 ggtctcgccg aactgcccgt tggcgagcct caacagaccg gccggattcg agaaactggc
     3061 cagcttcagc gtgtcgatga c
//
LOCUS       DATMXI010000135         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_111515, whole genome shotgun sequence.
ACCESSION   DATMXI010000135 DATMXI010000000
VERSION     DATMXI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111515"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VN444_02520"
     CDS             complement(<1..223)
                     /locus_tag="VN444_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20719.1"
                     /translation="MPTGERRLCAGYQEERLDGNLTSEGRRQSPVGEDSVAGWLETLR
                     GCRLQRQQSGADEAAPEISMVSQEFQRGAA"
     gene            349..1662
                     /locus_tag="VN444_02525"
     CDS             349..1662
                     /locus_tag="VN444_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20720.1"
                     /translation="MLETIKSIILDFQDARLETGVPRHVRIETVHGKAAVCIGVRRSG
                     KSTYMFQVIQRLLDGGVPRQNILYLNFFDDRLHTLRQDNLGLIAEAYYALYPEKKHTE
                     TVYCFFDEIQATPGWEPFVDRLMRTEQCEVYLTGSSARMLSKEIATQMRGRALSWELF
                     PFSFREFLDYKGVESEGALSTKKRLLVQKAFEAYWETGGFPEVAGLGRSLRIKIHQEY
                     LHAILFRDLVERHDVSHPRAVTDLAHWLVDNTASHYSVNRLTGYLKSLGHKVPKASVS
                     DYLEWFEDAYFLFSVRLFDPSLARSHTNPKKVYCVDHALVTSVSSGMLVNSGHLLENL
                     VFTALRRLHPEIYYYKTKSGREVDFIVPMRGRPRTLVQVCESLAEPQTRKRETTALGE
                     AMAELGFETGTIVTRNEAQRIKTSGSTIEVVPIWRYLLDLPDATA"
     gene            complement(1890..>2513)
                     /locus_tag="VN444_02530"
     CDS             complement(1890..>2513)
                     /locus_tag="VN444_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWR20721.1"
                     /translation="ALLCLVFVAVGCATARITEQRVVSGRITDQQGRPVPGTPVLLVG
                     RQLDFTTRFDYAELDRRGLKVLTADDGWYRAEFIPSELGNNLYLFFYAEKGFDSVRFQ
                     KPDGIDITKRLKEGRELRLDQTFLDHPQWKEVQRQIAQFGADSARGKILRQLGLPERI
                     DRGAGDQSTETWWYYNKGISYHFTGPVIGGSYTFQPIQGVLPLPLTK"
BASE COUNT          521 a          785 c          705 g          502 t
ORIGIN      
        1 gcgcagcacc gcgttggaat tcctgggaca ccatactgat ttctggggca gcctcgtctg
       61 cccctgactg ctggcgctga aggcgacagc ctcgtagggt ctccagccat ccggcaacgc
      121 tgtcctcccc tacggggctc tgcctgcgcc cttctgatgt cagattccca tcgagtcttt
      181 cctcctgata ccccgcgcag agacgcctct cgccggtggg caagcctgaa caatctgggg
      241 acgttcacac cgaccctggg aatggctctt gacgccaacg gcacaaaccg gctatattta
      301 tgaccagata acactattat cggaaataat tatgaccact gtgaccagat gctagagaca
      361 attaaatcca tcatcctgga cttccaggat gcccggctgg agaccggggt accccggcat
      421 gtgcgcatcg agacggttca cggcaaagcc gccgtctgca tcggagtgcg acggagcggc
      481 aagtcgacct acatgttcca ggtgatccag cggttactgg atggcggagt cccccgacag
      541 aacatcctat acctgaactt tttcgacgac cgcctccaca cgctacggca ggacaatctc
      601 gggctgatcg ccgaggccta ttacgccctc tatccggaga agaaacatac ggagacggtc
      661 tactgcttct ttgacgagat tcaggcgacc cccggctggg agcccttcgt cgaccgcttg
      721 atgcgcacag aacaatgcga ggtctatctc acgggctcgt cggccaggat gctctcgaag
      781 gagatcgcca cgcagatgcg cgggcgagcg ttgtcatggg agctgttccc gttctcgttt
      841 cgtgagttct tggactacaa aggcgtcgaa agcgaaggcg cgctgtcaac caagaagcgc
      901 ctcctcgtcc agaaggcctt tgaggcatac tgggagaccg gcgggttccc cgaggtcgcc
      961 ggccttggcc gaagcttgcg gattaagatc caccaggaat accttcacgc gatcctgttt
     1021 cgggacctgg tcgaacgtca tgacgtatcg caccccaggg cggtgaccga tctggcgcac
     1081 tggctggtgg ataatacggc ctcccactac tccgtcaacc gcctcacggg atatctcaaa
     1141 tcgctgggcc acaaggtccc caaggcctcg gtatcggatt acctggagtg gttcgaggat
     1201 gcctatttcc tgttcagcgt gcggctcttc gacccatcgc tcgcccgcag ccacaccaac
     1261 ccgaaaaagg tctactgcgt cgatcacgcc ctggttacgt cggtgtcgtc ggggatgctg
     1321 gtcaactccg gacatctcct ggagaatctc gtattcacgg cgcttcggcg tcttcacccg
     1381 gagatctact attacaagac gaagagcggc cgggaggtag actttatcgt cccgatgcgc
     1441 ggtcgcccgc ggacgctggt ccaggtgtgt gagtccttgg ccgaaccgca gacgcgaaaa
     1501 cgggaaacga cggccctggg cgaggcgatg gccgaattgg gctttgagac cggaaccatc
     1561 gtgacgagga acgaagccca gcgaatcaag accagcggca gcaccatcga ggtggttccg
     1621 atatggcgat acctcctcga tctgccggac gcgacggcat agctccatga gattcctact
     1681 gattcagaca cccacggatc attcggcatc atcgaccaat tgaccgcctg gcctgcctcg
     1741 cggcaggcgc ctgtctcaac gtcaccggaa tcctccatgc cgacctgtct gcgtgccccg
     1801 cttcgacagg ctcagggcaa acgggatgtg gaaagaagcg ttggatacac tacaccggta
     1861 aggtgacagg gcgaaagagg ttgcctgcgc tacttcgtga gaggaagcgg cagtacgcct
     1921 tggataggct ggaacgtata cgaacccccg atcacaggcc cggtaaaatg gtagctgatc
     1981 cccttattgt aataccacca cgtctccgta gattgatcgc cggctccacg gtcgattcgc
     2041 tccggaagac ccaactgacg caggatcttt ccccttgctg aatccgcccc gaactgcgcg
     2101 atctgtcgct gcacctcctt ccattgcgga tggtcgagaa acgtctgatc caatctcagc
     2161 tctctgccct cctttagccg cttggtgata tcgatcccat ccggcttttg aaaccggacg
     2221 ctgtcgaacc ccttttcggc atagaagaac aggtagaggt tatttccgag ttcgctgggg
     2281 atgaactcgg ccctgtacca gccgtcgtcg gcagtcagga ctttcagccc ccgccggtca
     2341 agttccgcgt agtcaaacct cgtggtgaaa tcaagctgtc gacccaccag cagcacaggc
     2401 gtcccaggca caggccggcc ctgttgatcg gtgatccggc cgctgacgac ccgttgctcc
     2461 gtgatccgag ccgttgcgca tccgaccgca acgaacacca ggcacaggag cgc
//
LOCUS       DATMXI010000136         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_267457, whole genome shotgun sequence.
ACCESSION   DATMXI010000136 DATMXI010000000
VERSION     DATMXI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267457"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN444_02535"
     CDS             complement(<1..254)
                     /locus_tag="VN444_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWR20722.1"
                     /translation="MRWLWVLMLLLPNLATAYEAQPLAANPQAEARLKALAVELRCLV
                     CQNQTLADSNAPLAEDLRREVREMIAKDMTDKEIIEFLVTR"
     gene            complement(251..778)
                     /locus_tag="VN444_02540"
     CDS             complement(251..778)
                     /locus_tag="VN444_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWR20723.1"
                     /translation="MSRFLIPLGIFVVVLAFLGVGLTLNPRELPSPLVGKAAPEFSLP
                     QLHDQGKTISSNDLKGKVWLLNFWASWCGGCKDEHPVLIHLAQSGVVPIYGMDYKDQR
                     NEALAWLKEWGNPYQIVAVDEPGRVGINYGVYGVPETYVIDKQGVIRYKQIGPLREDI
                     LEKKILPLVRELQNQ"
     gene            complement(775..>2121)
                     /locus_tag="VN444_02545"
     CDS             complement(775..>2121)
                     /locus_tag="VN444_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HWR20724.1"
                     /translation="MFLTLGIMLGSWWAYYELGWGGWWFWDPVENASFMPWLAGTALI
                     HSLAVTDKRGSFKAWSVLLAILAFSLSLLGTFLVRSGVLTSVHAFATDPKRGLFILVF
                     LAVVIGGSLMLYAWRAPRVGLGGSFSIVSREGMLLANNVLLVAALGAVLLGTLYPLFL
                     DALDLGKISVGPPYFDTVFVPLMTPAIFLMGIGPLAQWKKASLPELAVRLRWAFGVSV
                     ATALVVPILMGHWSPLVSLGLLLALWIATTIAVDLRERLTRTDGSSFMGKLAMLPRAY
                     WGMVIAHFGLALFITGVTMVKGFEVEQDVRMNVGETATLGGYTFRFDGTEQYRGPNYI
                     AARGSIHVSQDGRDVTVMYPEKRRYTVQNQTMTEAAISPGFLRDLYVSLGEPLEGGAW
                     SVRLYHKPFIDWIWGGCLVMAVGGVLAISDRRYRLAWRRKEVEVPAGTQMARQTVS"
BASE COUNT          503 a          669 c          561 g          388 t
ORIGIN      
        1 cgtgtgacga ggaactcgat gatctccttg tcggtcatat ctttggcgat catctcacgc
       61 acttctctgc gcaagtcttc agccaacggc gcgttcgagt cggccaaggt ttggttctga
      121 cagaccagac agcgcagttc gacggcgagg gctttcaaac gcgcctctgc ctgcgggttc
      181 gcggcaagcg gctgggcctc gtacgccgta gcgagattgg gaaggagcag catgagcacc
      241 cacagccatc tcactggttt tgcaactccc tgaccagagg caaaatcttt ttttccaaga
      301 tgtcctcgcg caacgggccg atctgcttgt agcggataac gccctgttta tcgatcacgt
      361 aggtttccgg cacgccgtag acaccatagt tgatgccgac gcgccccggc tcatcaaccg
      421 caacgatctg atagggattg ccccactcct tcaaccacgc cagagcctcg ttccgctgat
      481 ccttatagtc catgccgtag atgggcacca ctccggattg tgcgaggtgg atgagtaccg
      541 ggtgctcgtc cttgcagccc ccgcaccagg acgcccagaa gttgagcagc cagaccttgc
      601 ccttgagatc gtttgacgaa atcgttttac cttgatcgtg gagctgcggc agcgaaaatt
      661 caggggccgc ctttccgact aagggagacg gcaattcacg cgggttgagc gtcaggccga
      721 caccaagaaa cgcgagcacg accacaaaga tgccgagcgg gataagaaac cgactcatga
      781 gactgtctgc cgagccatct gtgtccctgc cggaacctcg acctctttgc ggcgccacgc
      841 gagtcggtac cggcgatcac tgatggctaa cacgcccccc actgccataa ccagacatcc
      901 gccccagatc caatcgataa agggcttgtg atagagccgg acactccagg ctccgccttc
      961 gagtggctca cccaacgaca catagaggtc gcgcagaaac ccgggagaga tcgcggcttc
     1021 ggtcatggtc tgattctgaa cggtgtagcg gcgcttttca ggatacatca ccgtcacgtc
     1081 acgtccatcc tgactcacgt gaatcgatcc acgcgccgcg atatagttcg gtcccctgta
     1141 ctgctcggtt ccatcaaacc ggaacgtata gccaccgagc gttgccgtct ctcctacgtt
     1201 catccgtaca tcctgctcaa cttcgaaccc cttcaccatg gtcacaccgg tgatgaacag
     1261 ggcaagtccg aagtgggcta tgaccattcc ccagtaggcc cgagggagca tggccagttt
     1321 ccccatgaag ctgcttccat ccgtacgagt aagcctttca cgcaagtcaa ccgcaatcgt
     1381 tgtcgcaatc cagagcgcca gtaatagacc aaggctcacg agcggcgacc agtgacccat
     1441 gagaatcggc accaccaggg cggttgcgac actcaccccg aaggcccaac gcagccgcac
     1501 cgctaattcc ggcaggctgg ctttcttcca ttgcgccaga ggaccgatcc ccatgaggaa
     1561 gatggcaggc gtcatcaatg ggacaaagac tgtatcaaaa tagggtggac cgactgaaat
     1621 cttaccaagg tccagcgcgt ccaggaacaa aggatagagc gtgcccaaga gcactgcccc
     1681 caaggccgca accagtaata cgttgttcgc cagtaacatc ccctcgcgtg aaacaattga
     1741 gaagctgccg cccagcccca cgcgcggcgc acgccatgcg tacaacatca gggagcctcc
     1801 gatgaccacg gccaggaaca ccagaataaa cagaccacgc tttggatcag tggcaaacgc
     1861 gtggaccgag gtcaagacac cggaacggac caagaaggtc cccaggagac tgagtgaaaa
     1921 cgccagaatg gcgagcagca cactccaggc tttgaagcta cctcgtttat ccgtgaccgc
     1981 cagggaatgg atcaatgctg ttccagccaa ccaaggcatg aacgaggcat tttcgaccgg
     2041 atcccagaac caccacccgc cccagccgag ctcgtaatac gcccaccagc tgccgagcat
     2101 gatgccgagc gtcaggaaca t
//
LOCUS       DATMXI010000137         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_146711, whole genome shotgun sequence.
ACCESSION   DATMXI010000137 DATMXI010000000
VERSION     DATMXI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146711"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN444_02550"
     CDS             <1..469
                     /locus_tag="VN444_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20725.1"
                     /translation="ASITRLLGRKKRSLAALLAPAKEVTLEGDRLIVMLENGTTFARS
                     TLDDLENRRLVTEAASEAFGRPMTVECRFDSPSNVPARSGSTQPTTGQGELRQPSRSI
                     EAHREARDPQQTAVPEPQMAQSDQESLRGHPLVQRAMELFDGQVVRVRAKQPE"
     gene            489..803
                     /locus_tag="VN444_02555"
     CDS             489..803
                     /locus_tag="VN444_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWR20726.1"
                     /translation="MKNLGNLMKQAQRMQAEAERIQAEAATKRVEGTAGGGIVTVVCN
                     GRGEVVEVKIDPEVAGPEELEMLQDLVVAAANEALRKARELLNHEMGRLTGGLGLPPG
                     LV"
     gene            811..1410
                     /gene="recR"
                     /locus_tag="VN444_02560"
     CDS             811..1410
                     /gene="recR"
                     /locus_tag="VN444_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWR20727.1"
                     /translation="MARYAPTMVRLVGELVRLPGIGPKTAQRLTFHLLKATKEEAVAL
                     AQAIIELKDRIQSCAVCYNISEGDQCPICADPTRDGSALCVVEEANDLWAIEKTTSFK
                     GRYHVLGGSLSPLEGRGPEQLTAKALLRRLEGGEVKEVILATNPNVEGEATAMYISRL
                     LKPLGVRATRIARGLPMGSDIEYADEVTLSRALEGRREI"
     gene            1594..1668
                     /locus_tag="VN444_02565"
     tRNA            1594..1668
                     /locus_tag="VN444_02565"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1626..1628,aa:Asn,seq:gtt)
     gene            1849..>2837
                     /locus_tag="VN444_02570"
     CDS             1849..>2837
                     /locus_tag="VN444_02570"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWR20728.1"
                     /translation="MPQHRALEQWVDQVARVTRPDRIHWCDGSEEEYQVLIEGMLRDG
                     TLIGLDHQRYPECYLHRSHPSDVARTEHLTFICTSRKEDAGPTNNWMEPKEARAGIGK
                     LFEGSMKGRTMFVVPYLMGPAGSPYSKIGVEITDSPYVVVNMRIMTRMGKVALERLGE
                     SDSFVRGLHSLGDLSPDRRFILHFPEERLIWSIGSGYGGNALLGKKCHALRIASWIGR
                     EEGWLAEHTLIIGVEDPSGQVTYMAAAFPSACGKTNLAMLVAPLHLSGYKVWTVGDDI
                     AWMHVGPDGRLRAINPEAGFFGVAPGTGVKTNPNMMAAIGRNVIFTNVAVTPDG"
BASE COUNT          593 a          787 c          921 g          536 t
ORIGIN      
        1 ggccagcatc acgcggctgc tgggacggaa gaagcggtcc cttgccgcat tgctcgcccc
       61 ggccaaggag gtgacgcttg agggagacag gctcattgtc atgctggaaa acgggacgac
      121 ctttgcgcga tcaacgctgg acgaccttga aaaccggcgc ctcgtgaccg aagccgcgtc
      181 cgaagccttt gggcggccca tgacggttga gtgtcgattc gacagtccaa gcaatgtacc
      241 tgcgaggagc ggttcgacgc agcccaccac aggccagggc gagctgcgtc agccttcgcg
      301 atctattgag gctcaccgcg aagcacgcga cccccaacaa acggccgttc cggagccgca
      361 gatggcgcaa tctgatcagg agtcgctgcg aggtcatccc ctggtgcagc gagctatgga
      421 gctatttgac ggccaagtcg ttcgggtcag agcgaaacag cccgaatagg gcgtgacagg
      481 agaatgaaat gaagaacctc gggaacctga tgaaacaggc gcagcgaatg caggctgagg
      541 cggaaagaat tcaggcggag gctgcgacga aacgggttga agggaccgca ggcggcggga
      601 tagtcaccgt cgtatgcaac ggtcggggcg aggttgtcga ggtgaagatc gatccggagg
      661 tcgccggccc ggaggaattg gagatgctgc aggaccttgt tgtggctgcg gcgaatgagg
      721 cacttcgaaa agctcgcgaa ctactgaacc atgagatggg tcggctgacc ggcgggttgg
      781 gactgccccc ggggctggtg tagagcagcc gtggcccgct atgcccctac catggttcgg
      841 ctggtcggcg agttggtccg ccttcccggc atcggtccca agaccgccca acgtctgacc
      901 tttcacctcc tcaaagccac caaggaggag gcggtggcgt tggcccaggc catcattgag
      961 ctgaaggatc ggatccagag ctgtgcggtc tgctacaaca tctctgaggg cgaccaatgt
     1021 cccatctgcg ccgacccgac tcgagacgga tcggcgctgt gcgtggtgga agaggccaac
     1081 gacctctggg ccatcgagaa gacgacgagc ttcaagggcc ggtaccacgt gctggggggg
     1141 tcgctgtcgc cgctggaggg acgcggccct gaacagctca cggccaaggc tcttctgcgg
     1201 cgcctggaag ggggagaggt aaaagaagtc atcctggcca cgaatccgaa cgtcgagggc
     1261 gaggcgacag cgatgtacat ctcgcgcctg ttgaaaccgt taggcgttcg ggcaacgcga
     1321 atcgctcgcg gcctcccaat gggcagcgac attgagtacg ccgacgaggt gaccctttcc
     1381 agggcactag aggggcgacg cgagatctga cgctcgcgga gtagaaagtg gttgctcgcg
     1441 ctcatcgggt gcgctaaatt acacaatgca gctttcagtt ttcagctatc agccatcagc
     1501 tttcagcaaa aatgggacga tgcgagattc gccgagaatc ctgccatcgc gctgaaagct
     1561 gactgctgat cgctattctt acgctgcctt tgatcccggg tagctcaacg gtggagcgag
     1621 cggctgttaa ccgctaggtt gtaggttcga gtcctacccc gggagccatt ttgttgttat
     1681 cggaaattga ctggctagtg tagtgcatcc aacacttctt taacatattc cgctcgccct
     1741 tgcctgtcga aagggtgaag tatttcaatg ggttccgttc atggttcgac cagctcacca
     1801 cgaacggaat gtaaaaggat ttatgatgca ctacacgagg gagacccgat gcctcagcat
     1861 agagcgttag agcagtgggt ggatcaggtg gcgagggtga cccgtccgga ccgcatccat
     1921 tggtgcgatg ggtcagagga ggaatatcag gtcctgattg aggggatgct acgggatggg
     1981 accctgatcg gactggacca tcagcgctac cccgaatgtt acttacatcg gagccatccg
     2041 tcggatgtcg ccaggaccga acacctgacc ttcatctgta cgtctcgaaa ggaggatgcg
     2101 ggccccacca ataactggat ggaaccaaaa gaggcgcgcg ccgggatcgg gaagctcttt
     2161 gaaggcagca tgaaaggccg gacgatgttt gtcgtcccct atcttatggg cccggccggc
     2221 tctccctaca gcaagatcgg cgtagaaatt acggacagcc cctacgtcgt cgtcaatatg
     2281 cggatcatga cccgcatggg gaaggtcgcg cttgagcggc ttggcgaatc agacagtttt
     2341 gtccgtggcc tgcactcgct tggtgacctg agtccggacc gccgcttcat cctccacttc
     2401 cccgaagagc gtctcatctg gagcatcggg tcagggtatg ggggcaacgc cctgttgggg
     2461 aagaagtgtc acgcccttcg tatcgccagt tggatcggtc gagaggaggg ctggttggct
     2521 gagcataccc tgatcattgg cgtggaagat ccgtcagggc aggtcaccta tatggcggcc
     2581 gcctttccga gcgcttgcgg caagaccaac ttggcaatgc ttgtcgcccc cctccatctc
     2641 tcgggctaca aggtgtggac cgtgggcgac gacatcgcgt ggatgcacgt cgggcccgac
     2701 ggacggctgc gggccatcaa tccggaggcc gggttcttcg gcgtcgctcc cggcaccggc
     2761 gtcaagacga atccgaacat gatggcggcg atcggccgaa atgtgatctt caccaatgtg
     2821 gccgtgacgc cggatgg
//
LOCUS       DATMXI010000138         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_285515, whole genome shotgun sequence.
ACCESSION   DATMXI010000138 DATMXI010000000
VERSION     DATMXI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285515"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..>2011
                     /locus_tag="VN444_02575"
     CDS             260..>2011
                     /locus_tag="VN444_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWR20729.1"
                     /translation="MTIRKLLSATVLSYPRTALVLAAILSILSVLIAIQRLRFTSTHD
                     ALSSLNGRMGQVQEQYNRAFGDPDRAVIVIEAKNREQAKRFAAALALRLKAMATDIEE
                     VIYRFDLRSLEDRFLMYLSPEALADLKGKLHEHRAMLEELSAEPGLNRLFQITRREIS
                     AALVGHLFTGFLDEQEGEVKRPVELAPLLKLLTQLNAWAAAPRTYTSPWSQFFVEANE
                     NGDREGYLWSNDKQFLFVLADVKADTTNLLKFERPIKGIRKEIRLLQSQYPEVKAGVT
                     GSPALEYDEVTAAQRDSGLMTAISLLGVALLVVVTFRSVARPLMGILALVMGVCWAFG
                     FAAVTVGHLNMLSMVLAPLLIGIGMDYGIHLLARYEEERAAGHSIRQALEQAFEGAGP
                     GILHAALTTSVALFTLILTGIGVLQELGVITGCGLLFTLISTFVVLPPLLILLDRRPI
                     VSSVEGRTGDACAPRRLLPRPPDFLEFCYRWPRAVLALSTIGTMAALYAVSSLEFDGN
                     VLRLQAEGTESVTWELKIIRQSERSISYGVILAKSLEEVREKSLALEALPSVSEVESI
                     AMVIPKDQERKLQLAREL"
BASE COUNT          405 a          569 c          597 g          440 t
ORIGIN      
        1 gaacactgac agctaaacgc tatcttctgg aaggctcctt ccagctcgtg gcggatggat
       61 acatattgaa agtgtaacta ctcaggtaga taggctgaag gctgaaggct tttaggggta
      121 agtcatgcgt gatcaaacaa aattccgaaa agagcttgaa tggctcgatt cgtgccttgc
      181 gagataattt agagccttca gcctaaacac cttgtagtta taagaagcga atggttgaac
      241 tctacttaag gtctaatcga tgacgatccg aaaactgctc tctgccacgg tcctcagcta
      301 tccgcggact gcactggtcc tggcggcgat cttgtccatc ctgtcagtcc tgatagcgat
      361 ccagcggctg cgtttcacct ccacacatga tgcgctgtct tccctcaacg gaaggatggg
      421 ccaggttcaa gaacaataca accgggcgtt tggcgatccg gacagggcgg tcatcgtcat
      481 cgaggccaag aatcgggaac aggccaagcg ctttgccgcc gcactcgccc ttcggctgaa
      541 ggctatggcc acggatatcg aggaggtcat ttaccgcttc gacctccggt cattggagga
      601 ccgattcctc atgtaccttt cccccgaagc gctggctgac ctgaagggga agctccacga
      661 gcatcgcgca atgctggagg agctgtctgc cgagccgggc ttaaatcgcc tgttccagat
      721 cacccgtcga gagatcagcg ccgccttggt gggtcacctg ttcaccggct ttctggacga
      781 gcaggagggt gaggtcaagc gtcccgttga actggccccg ctcctgaaac tgcttacgca
      841 actgaacgcc tgggccgctg ccccacggac ctacacgtca ccatggagtc agttcttcgt
      901 cgaggccaac gagaatggcg atcgggaagg gtacctatgg tccaacgaca agcagttcct
      961 gttcgtcctg gccgacgtga aggcggacac cacgaacttg ctcaagttcg agcgaccgat
     1021 caaagggatc cgcaaagaga tccggttgct gcagtcacag tatcccgaag tcaaggccgg
     1081 cgtaaccggg agtccggccc tcgagtatga tgaggtcacc gccgctcagc gcgattccgg
     1141 cctgatgacc gccatctcac tcctcggcgt ggccctgctg gtggtggtga cctttcgcag
     1201 tgtggcaaga cccctgatgg ggatcctcgc cctcgtcatg ggtgtctgct gggcgttcgg
     1261 gtttgccgcc gtcacggttg gccatctgaa catgctgtcg atggtcttag cgccactgct
     1321 gatcgggatc ggaatggact acgggatcca tctcctggcc cggtacgaag aggagcgagc
     1381 cgccggccat tccatccggc aggcgttgga gcaggccttc gagggcgctg gtccaggcat
     1441 cctccatgct gcgctgacaa cctctgtggc tctttttact cttatcctca cggggattgg
     1501 cgtattacag gagttgggcg tgatcaccgg gtgtggcctg ctgtttacgc tgatttcgac
     1561 cttcgtggtc ctgccaccgc tgcttatatt gttggacagg cgacctatcg taagttccgt
     1621 cgaagggagg accggggacg cgtgtgcgcc acgcaggcta ttgcccagac ctccagattt
     1681 tctggaattc tgctatcggt ggcctcgtgc cgtccttgcg ctgtccacga tcggcacgat
     1741 ggccgccctg tatgcggtga gtagtctgga attcgacggt aacgtcctcc gtcttcaggc
     1801 ggaagggacc gagtcggtga cctgggagct gaagatcatc cgacaatccg agcgctcgat
     1861 ctcctacggg gtaatcctgg ccaagagcct cgaagaggtg agagagaaga gtttggccct
     1921 cgaggccctt ccttctgtga gcgaagtgga gagcatcgcg atggtcatcc caaaggatca
     1981 ggaacgaaag ctgcagttgg ctcgcgagct c
//
LOCUS       DATMXI010000139         5123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_166996, whole genome shotgun sequence.
ACCESSION   DATMXI010000139 DATMXI010000000
VERSION     DATMXI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166996"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..922
                     /gene="cmk"
                     /locus_tag="VN444_02580"
     CDS             248..922
                     /gene="cmk"
                     /locus_tag="VN444_02580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWR20730.1"
                     /translation="MDGFIIAVDGPVGAGKSTAARLLAKRLGYRYIDSGAMYRALAWK
                     ALQAGLDLDDEPCLQRVADETCIALEPAGDRDLILVDGEDVSGQIRKREVEQASSRIS
                     THPGVRCVMVAHQQRMAAQGGIVMDGRDIGTVVFPDADVKFYLTARLEVRANRRHLEA
                     QAAGNTRGVEELAEEITARDERDMGRRASPLRKADEAVTIDTSDLRASEVIDAMEEEI
                     RRKAAT"
     gene            983..1636
                     /locus_tag="VN444_02585"
     CDS             983..1636
                     /locus_tag="VN444_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWR20731.1"
                     /translation="MWIAFFRGLSLKKAIAHSVSTTGRFLCLGIARVIFRLHIEGEEF
                     IPRTGPTILAANHASFIDPIIIMASVRRPVRFMAKKELFRFPLFGWLLRQFGTFPINR
                     NRINLQAFKQATSLLEAGEVVAMFPEGTRGDGIELRPAKPGIGLIAARTGVPVIPVLH
                     RGTEKVLPRGAWFPRPHRIVIKFGAPLRFGEGQTGKRQDQAELFSQTIMEKIAALKT"
     gene            1769..3505
                     /locus_tag="VN444_02590"
     CDS             1769..3505
                     /locus_tag="VN444_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HWR20732.1"
                     /translation="MMSETDKLEEAVYTSTDELDRELHDQEYSQELADLYAQSLQEIT
                     EGEIVKGTVLEIRNEMVLIDIGYKSEGAIPIKEFQTPSGELTVKVGDVVDVYLEQKED
                     NDGLIVLSREKAEKTKVWDDIRLAYEQGEAISGMILGRTKGGLTVDIGVRAFLPGSQV
                     DLRPVRDLDRLIGKSFPMKVIKLNQRRGNIVLSRRELLEEERRGLKDKTLQSLEEGKV
                     IRGKVKNITEYGAFIDLGGLDGLLHITDMSWGRVGHPSELFTVGDEIEVVVLKFDRAA
                     ERVSLGHKQRMKDPWEDVDQRFPVGSRIRGKVVSLTDYGAFVELAEGIEGLVHISEMS
                     WTQRVKHPSKIVSVGDSIEIMVLDVDKVNKRISLGLRQIEPNPWLSIEESYPVGTRVE
                     GTVRNLTDFGAFVELDEGIDGLIHVSDMSWTKRVRHPSEILKRGDKVEAVVLHTDKTN
                     RRISLGLKQSQPDPWQSTVLDKYRVGMDVKAKVVRLTDFGAFVELEDGVEGLLHISEL
                     SHERVAKPEDVVSIDQELSLRIIKLDANERKLGLSLRAYLDSQDAPNTNTDETSPDQR
                     PSDPQGEELEAS"
     gene            3521..4390
                     /gene="sppA"
                     /locus_tag="VN444_02595"
     CDS             3521..4390
                     /gene="sppA"
                     /locus_tag="VN444_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWR20733.1"
                     /translation="MKRTWLVVSLAVFVSLLLLFGLAFSLGRWEQMGGSKVALITVEG
                     VILDSKEVIEQLEKYRTNPSVKAIVIRINSPGGGVAPSQEIYEEILKSRQVNKTPIVA
                     SVGSLAASGGYYIASATDLIMANPGSITGSIGVLLQVPNISGLLQKIGVKSVVVKSGQ
                     HKDLASPTREMTDSERQILQTMLDDVHNQFIDVVAKGRQLDRKKVEAIADGRIFSGRE
                     AQSLGLVDQLGNLQDAIDRAGALAGIRGKPTVIQERKRRPFLLDLVRGLSPLNIDLPI
                     HPPLTLSVNYLLG"
     gene            4452..4733
                     /locus_tag="VN444_02600"
     CDS             4452..4733
                     /locus_tag="VN444_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWR20734.1"
                     /translation="MTKADLVELVAVQVGLTKKDTEVVIDTILESISNALASKDDGKV
                     ELRGFGSFRTRQRLARQGRNPQSGESVHVSPKRIPFFKPGKELRRLIDS"
BASE COUNT         1207 a         1237 c         1550 g         1129 t
ORIGIN      
        1 attggacgca atcctgccag gggcagccgt tccgttagag gcctagcgct ataccgataa
       61 ccgtcgcatc tcggcgtcct cgggtcgtcg ccgatcctca cgtacaaccg cgtacgctcc
      121 ggtcggctcc ttcctgcgtc cttgatctgc tcgattctgg gcatggcgca ggtcatagga
      181 atcgtgcaga tgttgggcgc gcaagcagca tagcgctgag ggagagaacg accagttgag
      241 aaggacgatg gacggtttca ttattgcggt cgacgggccc gtaggggcag gaaagagcac
      301 ggcggcgcgc ctgctggcca agcggctagg gtaccgttac atcgacagcg gggccatgta
      361 tcgtgcgctg gcgtggaagg cgctgcaggc gggacttgat ctggacgatg agccatgcct
      421 acagcgggtg gccgatgaaa cctgcatcgc gctcgaacct gccggcgatc gagatctgat
      481 tcttgttgat ggtgaggatg tcagcgggca gatcaggaag cgcgaggtag aacaggcgtc
      541 ttcgagaatc tctacccacc cgggtgttcg ttgtgtgatg gtggcacacc agcaacgaat
      601 ggctgcgcag ggagggatcg tgatggacgg gcgtgatatt ggtacggttg tcttcccgga
      661 cgctgatgtg aaattctatc ttaccgctcg tttagaggtg cgggcgaacc gccggcacct
      721 tgaggcgcag gcggcaggta atacccgagg ggtggaggag cttgccgagg agattacggc
      781 gcgagatgaa agagatatgg gccgacgcgc ctcgccactg cgaaaggccg acgaggctgt
      841 gacgatcgat actagcgatt tgcgagcctc cgaggttatt gatgcgatgg aagaggaaat
      901 cagacggaag gcggctacct gaaaagcctt catgtgagtg aggcgaattt gttcttgcct
      961 ttgcgggagg taatcctata tcatgtggat tgcttttttt cgggggcttt ctctcaagaa
     1021 ggcgatcgct cattccgttt ctactacggg tcgctttttg tgcctcggta tcgcaagggt
     1081 catattccgc ctccacatcg agggggagga gtttattccc cgtactggtc caacgatcct
     1141 ggcggcgaat cacgcaagct ttatagatcc catcattata atggcctcgg tgcggcgtcc
     1201 ggtccgtttc atggctaaaa aggagttgtt tcgtttcccc ttatttggtt ggctgcttcg
     1261 gcagttcggt acatttccaa tcaatcggaa ccgaatcaac ctgcaggcat ttaagcaggc
     1321 gacctcgctt ttggaagcag gagaggtcgt tgcaatgttt cctgaaggga cgcgcggaga
     1381 tggaattgag ttacggcctg ccaagcctgg gattggactg attgcggcgc gaacgggcgt
     1441 accagtgatt ccggtgttac atcggggaac ggagaaggtg ctccccagag gcgcatggtt
     1501 tccaaggccg catcggatcg ttataaagtt tggggcgcca ttacggtttg gcgagggaca
     1561 aacagggaaa agacaggacc aggccgagct gtttagtcag accattatgg aaaaaatcgc
     1621 cgctcttaag acctgatcag ggagcggttc tcaatgagga catgattgcg cttgcactac
     1681 aggagacgct atgccgacaa agaagcgagg gatcaatgag taaacgtgga aaggattcgc
     1741 accttatcaa agggaggaac taaagggtat gatgtctgag acggacaagt tggaggaggc
     1801 tgtgtacaca tcaacggacg agctcgatcg cgagcttcac gaccaagagt actcccaaga
     1861 actggctgat ctctatgccc agagcctgca ggaaatcact gagggggaga tcgtcaaggg
     1921 aactgtgctg gagatcagga acgaaatggt cctgatcgat atcggctata agtctgaagg
     1981 ggccatacca atcaaggagt tccagacccc ttctggagag ctcacggtca aggttgggga
     2041 tgttgtcgat gtctacctgg agcagaaaga ggataacgac ggcctcattg tcctctcgag
     2101 agagaaagca gagaagacca aggtctggga tgatattcgg ctggcctatg agcaaggcga
     2161 agcgatcagc ggaatgatct tgggccgcac aaaaggtggc ttgacggtgg atattggcgt
     2221 gagggccttt ctcccaggct cccaggttga tctccgacct gttcgtgacc tcgatagatt
     2281 gatcgggaag agcttcccga tgaaagtgat aaaattaaat cagcgtcgag gcaatatcgt
     2341 attgtcccga cgggagttac tcgaggaaga gcggcgaggc ctcaaggaca agaccttgca
     2401 gtcgctggaa gaggggaaag ttatccgggg caaggtcaag aacattaccg agtacggcgc
     2461 gttcattgac ctcggcggac tggacgggtt gctccatatc accgatatgt cctggggtcg
     2521 cgtcggtcat ccctccgagc tctttactgt cggcgatgag attgaggtcg tggtccttaa
     2581 gttcgaccga gcggcggaac gcgtctccct tggccataag cagcgcatga aggatccgtg
     2641 ggaggatgtc gatcagcggt ttcctgtcgg ctcccgtatt cgaggcaagg tggtgagctt
     2701 gaccgactat ggcgccttcg ttgagctcgc ggaggggatc gaggggctgg ttcatatctc
     2761 cgaaatgtcc tggactcaga gggtaaaaca cccctctaag atcgtctcag tcggggactc
     2821 catagagatt atggttttgg acgtagataa ggtcaacaag cggatctcat tggggcttcg
     2881 tcagattgaa cccaacccat ggttgtcaat cgaggaaagc tacccggtgg ggacgcgcgt
     2941 cgaagggacc gtcaggaacc ttaccgactt tggcgcgttc gttgagttgg acgaggggat
     3001 agatggactg attcatgtgt ccgatatgtc ctggaccaag cgggtccgcc acccctctga
     3061 gatcttaaag cgaggagaca aagttgaggc ggttgtcttg cacaccgata aaacgaatcg
     3121 tcgaatctct cttggcctga agcagagcca acccgaccca tggcaatcta ccgtcctcga
     3181 taagtaccgg gtaggcatgg atgtgaaagc caaggtggtc cgtctgaccg atttcggagc
     3241 gttcgtcgaa ttggaagacg gggtggaagg cctccttcat atctctgaac taagccatga
     3301 acgggtcgcg aagcccgagg atgtggtctc catcgaccag gagctctcgc tcaggatcat
     3361 caagttagat gccaacgagc gtaagcttgg gctgagcctt cgcgcctatc tggacagcca
     3421 ggacgctccc aacacgaata ccgatgagac ctccccagat cagcgtccat cagatcccca
     3481 gggggaggaa cttgaagcct cgtagagggc gagtggggca atgaaacgga cttggttggt
     3541 cgtcagcctg gcagtgttcg tgagcttgct gctcctgttt ggcttggcct tctcgcttgg
     3601 cagatgggag cagatgggcg gaagcaaggt tgcgctgatc acggtcgaag gggtaatcct
     3661 ggattcaaag gaggttatcg agcagctcga aaaataccga acgaatccat ctgttaaggc
     3721 catcgtcatc aggatcaaca gtccaggggg cggggtggcg ccatcccagg agatttatga
     3781 agaaattctc aagagtcgtc aggtcaacaa gacacctata gtcgcgtccg tgggaagcct
     3841 tgctgcgtca ggtgggtact atatcgcgag cgcaacggat ctcatcatgg ctaatccagg
     3901 atctatcact ggaagcatag gggtgctgct acaagttccg aatatctctg gtctcttgca
     3961 aaagatcgga gtgaagtcgg tggtcgtcaa gagcggccag cacaaggatc tcgcttctcc
     4021 cacccgcgaa atgactgatt ctgagcgcca gatacttcaa acgatgcttg acgatgtaca
     4081 taaccagttt attgacgtgg tagccaaggg tagacaactg gatcgtaaaa aggtcgaggc
     4141 catagcggac gggcgaattt tcagtggacg agaggcgcaa tcgcttggat tggttgacca
     4201 acttggaaat cttcaggacg ctattgaccg ggcaggcgct ctggctggaa tccgcgggaa
     4261 accaacagtg attcaggagc ggaagcggcg acccttcctc cttgatctgg tccgcggtct
     4321 gagccctctt aacatcgatc tgccaattca tccaccctta accctctctg taaattatct
     4381 tctagggtga ttgcaccgta ccaactgatg agtgaccgat acagttcgtg aatatgaagg
     4441 agtttccagt gatgacaaag gcagacttgg tagaattggt tgctgtccaa gtgggtctca
     4501 ctaaaaaaga taccgaagtc gtcattgata ccatcctcga aagcatctcg aacgcgttgg
     4561 cttcaaagga tgacgggaag gtggagctcc gtggcttcgg tagcttccgc accaggcaac
     4621 gtctcgcacg gcaaggccgc aatccgcaaa gcggagaatc ggttcacgtt tccccaaagc
     4681 gaatcccctt ctttaagcca ggcaaagaat tgaggcggct catcgacagc tagccttcgg
     4741 aacagcgtta tccctcacct ccgccctcat cctccccagt cgcgggagag gggagttctg
     4801 attcgtacct gcccgtgcgt agccgtacgc agagaggttc acctacgtaa aatcggaaga
     4861 atcaaatcta cgtatttgcc acgacaacga tgtcgtaaaa agagcttgcg acagcgaagc
     4921 tcgaatgtta aatttagctt ggttggtcat gtagataggg tagagactga cgcgtggggc
     4981 ttcttgccca atgaggggtc ctgtctcaga gaatcaaggg tcgtagaacc cgaaaaggtc
     5041 ttggctggct tgattcgtgc tttacgagat catgtataac cttcagtttt cagcctaaat
     5101 gcctaatagt tatggaatca tta
//
LOCUS       DATMXI010000140         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_41418, whole genome shotgun sequence.
ACCESSION   DATMXI010000140 DATMXI010000000
VERSION     DATMXI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41418"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1513)
                     /locus_tag="VN444_02605"
     CDS             complement(140..1513)
                     /locus_tag="VN444_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWR20735.1"
                     /translation="MSEKSSLYPELIRTIKPATGTLGVLLPGLGAVATTLIAGVHLIN
                     KGLAQPHGSLTQMQRMRLGKRTNPRFALIKEVVPLTDLNDLVFGGWDIFPENAYQIAC
                     HAGVLSRELLDQVKDQLESVRPWPAVFEQAYVRRLSGPHLKSGPSKKHLADMLIEDIE
                     AFRRREGVERAVMVWCGSTEVFMQPSPVHETIEAFEAGLKANAPEISPCMIYAYAAIS
                     AGVPFANGSPNLAVDIPALVEYARKRRVPIAGKDFKTGQTFLKTVVAPGLKAKMLGLT
                     GWFSTNILGNRDGEVLDDQGSFRTKEVSKSSVLGAILQPELYPDLYGQVFHKVRIEYY
                     PPRGDAKEGWDNIDIFGWLGQPMQIKINFLCRDSILAAPVALDLALFLDLAQRAGLSG
                     IQEWLSFYFKSPMARADLKPEHDLFIQHLKLTNTLRILIGEEVLDHSGLDYYESGDAY
                     RAGMGGA"
     gene            complement(1510..2703)
                     /locus_tag="VN444_02610"
     CDS             complement(1510..2703)
                     /locus_tag="VN444_02610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWR20736.1"
                     /translation="MKGTTALIMMTKDSGEISPDTVVAGIPLLRRIVMAASRAGCHEI
                     LVLDTGWPGLMKLLTGTRAVLLPPDKLTDYPHSSRILVLAGNLLPRPSLLTHLTEMPL
                     RAETMHVPVSGIAAVETADPKAMLSMIGRAGDDLSVFSGLERTYQRAGTLIDGAGWVR
                     VSNRADLRNAERWLLRGLVKDTEGFMSRHFERKISLAVTRCLVRTGITPNQMTAVSVA
                     IGLLGALFFLSSMPAYQLTGALLFLLHSILDGCDGEIARLKYLESRLGGILDFWGDNV
                     VHSAVFVCIGLGWQMAIKAPVPLVLAGSAVIGTLLSAGFVYRQTMRNKVSEGPLFTSI
                     TNSEAPSRLTTLADSLARRDFIYLVVILSALGKAHWFLVLAAFGAPLFFFILLWTSRR
                     ERVKA"
     gene            complement(2772..>3354)
                     /locus_tag="VN444_02615"
     CDS             complement(2772..>3354)
                     /locus_tag="VN444_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR20737.1"
                     /translation="SRACPESSRGGASHDPRSASRIGNWRLDPAMAGGGILLDHGWHA
                     MYIILRWLCQNPRTISATLEKRAHPEWPVEDTATVQVNFPEAVADIFLTWDASSRGNS
                     AVIEGTCGAIRVDDDILLLTGKSGEQRWKFEQSLSQGSHHPEWFCQVRDDFLQEISGA
                     ERSGENLATASRCAQLLHLAKESHAIGGRHLSV"
BASE COUNT          718 a         1040 c          972 g          624 t
ORIGIN      
        1 cgataaggtc atccgggaca agtcgtatga agacctcgtc aagaagatgc gcctcaagcg
       61 ggaggcggag gggctgggat cgacagcaaa gaaggggtcg atgcagtagc tccttcgatg
      121 aggccatgac agagctgctt caagccccgc ccatgccggc ccgataggca tctccggatt
      181 cgtagtaatc aagcccggag tggtcaagta cctcctcccc gatcaggatg cgcaaggtat
      241 ttgtcagctt cagatgttgg atgaacaggt catgctccgg cttcaggtcc gctcgggcca
      301 tcggagactt gaagtagaac gagagccact cctgaatgcc cgataatccg gcccgttgtg
      361 ccaggtccag aaacagagcc aggtccagag ccaccggcgc tgccaggatg gagtcccgac
      421 agaggaagtt gatcttgatc tgcatcggct gaccgagcca gccaaagatg tcaatgttat
      481 cccacccctc tttggcatca ccccgcggcg gataatactc aattcggacc ttgtggaaca
      541 cctggccata caggtccgga tagagctccg gctgcaggat cgcgcccagc accgagctct
      601 tgctcacctc cttggttcta aacgatccct gatcatccag cacctcgccg tcccggttcc
      661 ccaggatgtt ggtggaaaac cagccggtca gccccagcat cttggccttc agccctggcg
      721 cgaccacagt tttcagaaag gtctgtcccg tcttgaagtc cttcccggcg atcgggacgc
      781 gccttttgcg agcgtactct accagcgccg ggatatccac ggccagattg ggtgagccat
      841 tcgcaaaggg cacgccagcc gagatagcgg cataggcata gatcatgcac ggggagatct
      901 ccggagcgtt cgccttcagg ccagcctcaa aggcctcgat cgtctcgtgt acgggggacg
      961 gctgcatgaa gacctctgtt gatccacacc agaccatcac ggcccgctcg accccctccc
     1021 tccggcggaa tgcctcgata tcttcgatca gcatgtccgc caggtgcttc ttgctcgggc
     1081 cggatttgag gtgcggtcca gacaacctcc gcacataggc ctgctcaaag accgccggcc
     1141 acggcctgac agactccagt tggtctttga cctggtccag caactccctg gacagaaccc
     1201 cggcatggca cgcaatctga taggcgttct caggaaagat gtcccagcca ccgaagacca
     1261 gatcgttaag atcagtgagg gggaccacct ccttgatcag ggcaaaccga gggttggtcc
     1321 gcttgcccag gcgcatccgc tgcatctgcg tgagggaccc gtgcggttgg gcaagccctt
     1381 tattgatcag gtggacccct gcgatcaggg tagtggccac cgcccccaac cccgggagca
     1441 gcaccccaag cgtacccgtg gccggtttaa tcgtcctgat cagttctgga tagagagaac
     1501 tcttttcgct catgccttta ccctttccct gcgtgaggtc cacagcaata tgaagaaaaa
     1561 gagcggcgca ccgaaggcgg ccagcaccaa aaaccaatga gcctttccta atgccgatag
     1621 gatgacgaca aggtagatga aatcccgcct tgcgagggaa tcggcaaggg tggtaaggcg
     1681 ggatggggct tctgagttgg tgatggaggt aaagagcggc ccctcggaaa ccttgttacg
     1741 catggtctgt cggtagacga aaccggccga caggagggtt ccgatcaccg cggaacccgc
     1801 taagaccaag gggacgggag ccttgatggc catctgccat cccagaccga tgcatacaaa
     1861 gactgccgag tgtacaacat tatctcccca aaagtccagg atgccgccca ggcgagattc
     1921 cagatacttg agcctggcga tctccccatc gcagccgtcc aggatcgaat gcaagaggaa
     1981 caggagcgcg ccggtcagtt gatacgcggg catggacgag aggaagaaca gggcccccaa
     2041 tagacctatc gccacgctca cggctgtcat ctggttcggg gtgatgcccg tgcgcacgag
     2101 gcaccgggtg actgccagtg agatcttccg ctcgaaatgc ctcgacatga agccttccgt
     2161 gtctttgacc agaccgcgca ggagccaccg ctcggcatta cgaagatccg cccggttcga
     2221 gacccggacc cagccagcgc catctatgag ggtgcccgct ctttgatagg tccgctccag
     2281 tccggagaat acagacagat catcgccagc acgcccaatc attgacagca tagccttggg
     2341 gtcagccgtc tcaacggcag cgatcccgct gactgggaca tgcatcgtct cagcgcgtaa
     2401 cggcatctcg gtgagatgcg tgagcagcga ggggcggggg aggaggttcc cggcgagcac
     2461 caggatacgg ctggagtgcg ggtaatcggt aagcttatct ggaggaagca gcacggcccg
     2521 cgttcctgtc aatagcttca tgagcccggg ccagccggta tccagtacca gaatctcatg
     2581 gcagcccgcg cgggaggcgg ccatgacaat gcgccgaagc aacgggatgc cagccacaac
     2641 ggtgtccgga gagatctctc ccgaatcctt cgtcatcatg atcagggccg tcgttccctt
     2701 catgaccata cgttcacacg caatgtaggg gcgctgcttg ctgcgccctc tgggcagggc
     2761 aagccctacc cctacacgga caaatgccgg ccccctatgg cgtgcgattc cttggcgagg
     2821 tgcagaagtt gcgcgcaacg cgaagccgtg gcgagattct ctccgctccg ctcagcgccc
     2881 gaaatctcct gcagaaaatc gtccctcacc tggcagaacc actccggatg gtgggagccc
     2941 tgcgagagcg actgttcgaa cttccatctc tgctcaccac tcttacctgt caggagcagg
     3001 atgtcgtcat cgacccggat ggccccacag gtcccctcga tcacagcgct attgccccgt
     3061 gaagacgcat cccacgtcag aaagatatcg gctactgcct ctggaaagtt cacctggacg
     3121 gtcgctgtat cctcaacagg ccattctgga tgcgctcgct tctcgagcgt ggcggagatc
     3181 gtcctcgggt tttgacaaag ccagcgcagg atgatgtaca tggcgtgcca accgtggtca
     3241 agcaggattc cgccacccgc catagccgga tcgagtctcc agttgcctat cctcgacgcc
     3301 gatcggggat cgtgcgatgc tccccctcgg ctagactccg ggcaggcccg gctg
//
LOCUS       DATMXI010000141         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_221225, whole genome shotgun sequence.
ACCESSION   DATMXI010000141 DATMXI010000000
VERSION     DATMXI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221225"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..767)
                     /gene="fsa"
                     /locus_tag="VN444_02620"
     CDS             complement(120..767)
                     /gene="fsa"
                     /locus_tag="VN444_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWR20738.1"
                     /translation="MKFFLDTANVKEIQEAASLGLLDGVTTNPSLVAKEGRSFKEMLV
                     EICNIVDGPISAEVVSLEADAMVKEGKGLAKIHKNIVVKVPLIAEGLKATKRLASEGI
                     KVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQILTIYRNYDYKTLV
                     LVASVRHPQHVVEAALAGGHICTMPFSIFQQMVKHPLTDSGLKKFLADWEGQAKK"
     gene            964..1275
                     /locus_tag="VN444_02625"
     CDS             964..1275
                     /locus_tag="VN444_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20739.1"
                     /translation="MQRGVWVLLFSLALAGCGGSNPYLEQSLKPAELQGKDKAWFEKN
                     WGAPSGKAPRFFGGETWTYYRIAGGKSGPPLFNFSPNECQITLKFDKEEKLSDYSYSG
                     C"
     gene            complement(1272..1532)
                     /locus_tag="VN444_02630"
     CDS             complement(1272..1532)
                     /locus_tag="VN444_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWR20740.1"
                     /translation="MYRIFLAIGLLIVLYFLVRRAVTAVTKIKGRSEPDRLPPGKNHM
                     VQDPVCLVFVPRGTAITEEIGGQTYYFCSQSCAHKFQEKLAG"
BASE COUNT          500 a          589 c          545 g          506 t
ORIGIN      
        1 atccgagcca gcaaaatacc ttcacaggaa tactgaccag gccgatgttt caccatatct
       61 ttgcaaccgc tcgacaacga cttgccgagc cggaacgcac acgagccggc caataggcct
      121 tacttcttgg cttgcccctc ccagtccgcc agaaacttct tgagtccgct atccgtcagc
      181 ggatgtttca ccatctgctg aaaaatcgag aagggcatcg tacagatatg cccaccagct
      241 agcgcagcct cgactacgtg ctgaggatga cgaacgctgg ccaccagcac cagggtttta
      301 tagtcataat tgcgatagat ggtcaggatc tgtcggatca gttccatccc gttcgaactg
      361 atgtcgtcga gtcgaccgat gaagggtgac acgcaccagg cgccagcttt tgccgccagg
      421 agtgcctggg tcggagaaaa gcacaacgtg acgttcacct tgataccttc ggaggccaat
      481 ctcttggtgg ccttcaatcc ctccgcaatc aacggcacct tcacgacgat gttcttgtgg
      541 atcttcgcaa gccccttccc ttccttcacc atggcatcgg cttcgaggct gacgacttcc
      601 gcgctaatcg gcccgtccac aatgttgcag atctcgacga gcatttcctt aaaactgcgc
      661 ccttccttgg cgacgagcga cgggtttgtc gtcactccat ccagaagtcc gaggctggct
      721 gcctcctgaa tttctttgac gttggcagta tcaagaaaga atttcatggg cttgtccttt
      781 catgtcgtga tgagaaattt tcacgattga ttcgttatcg gctattctat gaagaactca
      841 acctgactgc aatctcctgc cggcatacga cggtttgaca ggctccgaac ggccggttag
      901 aattcgattg ctcccgatgg cttgttactt catgtatctt taccgccgat aggagaatgc
      961 gcgatgcaac gaggggtttg ggtgctccta tttagtctgg ccctggccgg gtgcggcggt
     1021 agcaacccct atcttgaaca atcactcaag ccagccgaac ttcaaggtaa ggacaaggcc
     1081 tggtttgaaa agaactgggg ggcaccgagc ggcaaggccc cgagattttt tgggggcgaa
     1141 acatggactt attatcgtat tgccggtggc aagtcaggcc cgcctctgtt taatttttcc
     1201 cccaacgaat gccagatcac gctcaaattc gacaaagaag agaaactctc cgactacagt
     1261 tactcgggct gttagcctgc gagcttttcc tgaaacttgt gtgcacagga ctggctgcag
     1321 aagtaatacg tctgcccccc gatttcttcc gttattgccg ttccccttgg aacaaatacc
     1381 aggcagacgg gatcctgtac catgtgattt ttaccaggag ggagccgatc cggttcgctc
     1441 cgccccttga tcttcgtgac ggcggtgacg gcgcgccgga cgagaaagta cagtacaata
     1501 agaagcccga tagcgaggaa aattctatac atagtggtgg acttttctta acaccacaat
     1561 cctatgggac agcaccacgg ctgtcaagga cccatgcccg gttcagggct attcgttaac
     1621 gaactcccat tccttccctc tcctcaaatg gcctagctcc atatgagcgg aagagcctac
     1681 ctctccagga agacccagta cttcaggtgg atatgcttcc atgccttagt atggtagatt
     1741 gccgccatca tgatgatttg tgacaaatgc cgtggggcga tgttaccgga acgggcagtg
     1801 gacctgaacg caggtcttgc aatcacagtg tttgcttgtt tgaattgtgg tcgcaggaaa
     1861 tcagcagatc aggaacctcg gccgattacc gcgagacatt aaccggcgct atgatagcga
     1921 aaccgctcac gtacgacgag cataaggcag ctgaagctgc ctttcgcgga tgtccgccga
     1981 gcgaggattg gacggactcc gcctatgaag tttatgcccg tctatctgct gcgatcgcca
     2041 aacgaagggc gatcaatctt aatcaggcag acgaacctga ccttgaggaa gtcactcgat
     2101 agtttgtgaa gcgagctatc acctgcacgt agtgcagaat
//
LOCUS       DATMXI010000142         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_91458, whole genome shotgun sequence.
ACCESSION   DATMXI010000142 DATMXI010000000
VERSION     DATMXI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91458"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VN444_02635"
     CDS             <1..696
                     /locus_tag="VN444_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWR20741.1"
                     /translation="VAMFAGALAASLFGLGIGALTLRMRGPYLALTTLAFSEILRIFI
                     TAEYQLTQGSMGLKAPLMFAGDKNYCYYAGLFLFGLIYVLLQVLLRSRTGLFLVAMRE
                     DEDGAATRGVNTVAFRLLAFVVTSAAAGLAGGFYAHVVGLVSPQMIRIGEMGLVLAMS
                     VFGGLESLLGAALGAVALQALTEYLRVFVEWRLAIFSALVLLTLKFAPEGILPRFFKH
                     SERLVGRILGWSQ"
     gene            693..1412
                     /locus_tag="VN444_02640"
     CDS             693..1412
                     /locus_tag="VN444_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR20742.1"
                     /translation="MKIMTIQALSKSFGGLQALSNVNVSFESGEFTGLVGPNGSGKTT
                     LLNCISGLYSPDKGEIEFSGRRIASLPAHRICKLGIGRTFQVSKVFNRLTVADNLKIP
                     ALAEGRLSARELHNRASEILKQIKLEHLSRSNAENLSGGQRKLLELGMLLMTDPELLL
                     LDEPFGGVHPELKSELEDYLLLLNRSGKTIILVSHEMPSVFKICRRLVVLDRGLLLTE
                     GNPEAVRSDERVINAYLGGQA"
     gene            1409..2122
                     /locus_tag="VN444_02645"
     CDS             1409..2122
                     /locus_tag="VN444_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013962054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR20743.1"
                     /translation="MKLSVTDLHAAYQRDLFILRGVDIEVAEGSITIVIGPNGSGKST
                     LLKSIAGILNPRKGTVVLGGNEITGIELNSIIRQGIAYIPQDRAVFPQMTVSENLELG
                     CWIFRKEQARVRAQVKWALDRFPALRDKSDFLAGSLSGGLQRTLDIAKALLSDPSLLL
                     VDEPSVGLSPVMAQAIYSELLSLKKEGRTILLVDQDVRSAMQIADYVYVLELGKVKAH
                     APRQAFEGKLGEIVRSWLI"
     gene            2132..3307
                     /locus_tag="VN444_02650"
     CDS             2132..3307
                     /locus_tag="VN444_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWR20744.1"
                     /translation="MSPLDGKSILATIETICSFGTRWMGGDGAEQTLRHAIAEFKRAG
                     LKVELQPFRYPHYQPLGAGISVEGHALLCEPVALSGSNATPFEGQLVYGGTCSHDELQ
                     EIRDKGIDLADAIVLSDNLRSFVAYPEVEAAGAAGFVSMTNLPEDTIRCGCARLDGKV
                     GTIPAVAIGGADRHHILTRMSSGASLRAVLQSQGSIEERQGFNVVASLAGVSLPKILV
                     TAHYDCFWNGVMAMDNCAGVAIVLALSQTFTPMMLSGTEFVLFGAEEVGCWGASGYIR
                     QRSSSLGGLKGNLNLDTFGSNRSGIEVGCTSDLEALCRSVANQTGIAVDVWSIPPRSA
                     SDHQKFVENGYPAVWIANGGTDTRYHTPLDIPSEMSEESLETVGRLAYSLAAKLLGS"
     gene            complement(3833..>4347)
                     /locus_tag="VN444_02655"
     CDS             complement(3833..>4347)
                     /locus_tag="VN444_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR20745.1"
                     /translation="RQAVANCHKYGIMVVAGMIFGFPDDDEEDIIENYRFLKAIEADT
                     SYCQILTPYPKTGMRQYLSDEGLVTNADDFTRYNGMWANVRTRHLSAEQLQYLVWYHQ
                     QNVMGWWEPSERVRKRGPLWTGIWIYAFRPFLKVFLGRQLRKYGWRGRYERHIQRIPE
                     VNRFRDLDLA"
BASE COUNT          869 a         1210 c         1278 g          990 t
ORIGIN      
        1 gtcgccatgt ttgcaggtgc cctcgcggcc tcgctctttg ggctcggtat cggtgcgctt
       61 acgttacgga tgcgcgggcc ttatctcgca ctgacgaccc ttgccttctc cgagatcctc
      121 cgcattttta ttaccgccga gtaccagttg acccagggtt cgatgggcct gaaggccccg
      181 ctcatgttcg ctggagataa aaactattgt tattatgccg gcctttttct ctttgggctc
      241 atctacgttt tgctgcaggt tctgcttcgg tccaggacag ggttgtttct ggttgcaatg
      301 cgggaggacg aggacggtgc ggccacgcgc ggcgtcaaca ctgtggcctt caggctgctg
      361 gccttcgtgg tgacgagtgc cgcggcagga ctcgcgggcg ggttttacgc gcacgtggtc
      421 ggcctcgtga gcccacaaat gatccgaatc ggggagatgg ggctggtcct cgccatgagc
      481 gtgttcggag gactcgagtc gctactgggt gcggccttag gggcggtggc gctgcaggca
      541 cttactgaat atctccgcgt gtttgtcgaa tggcggctcg ccatttttag tgcgttggtg
      601 ctcctcacgc ttaaattcgc tcctgaaggg attctgcccc ggttcttcaa gcattccgag
      661 cgcctcgtgg gtcgcatctt gggatggtcc caatgaagat aatgaccatc caggcgttga
      721 gcaaatcctt cggtgggctg caggcccttt caaatgtcaa cgtctccttt gaaagcggcg
      781 aattcaccgg gctcgtcgga ccgaacggct ccggcaagac aacgcttttg aactgcattt
      841 ctgggctcta cagcccggat aagggcgaga tcgaattcag tggtaggcgc atagcaagcc
      901 tcccggcgca tcggatttgc aaacttggga tcggtcgcac atttcaggtt tcgaaggtct
      961 tcaaccggtt gactgtcgcc gacaacctga aaatacccgc gctcgctgag ggccggctga
     1021 gcgcgcgcga gctgcacaac cgtgcctccg agatcctgaa gcagatcaag ctcgagcacc
     1081 tatcgcgctc taacgccgaa aacctttctg gcggtcagcg taagctcctc gagcttggga
     1141 tgctcttgat gacagacccg gagttactgc ttctggacga accctttggc ggggtgcacc
     1201 cagagctcaa atcggagctg gaggactatt tgctcctcct aaaccgcagc gggaagacga
     1261 tcattctggt tagccacgaa atgccttcgg tgttcaaaat atgcagacga ctggtcgtgc
     1321 tcgaccgcgg tttgctcctg accgaaggca atcccgaggc cgtccgctcc gatgagcggg
     1381 tcatcaatgc ctatctcgga gggcaggcat gaagctctcg gtaactgacc tgcatgccgc
     1441 ctaccagaga gatctcttca tactgagggg tgtcgatatc gaagtggccg agggcagcat
     1501 caccatcgtt atcgggccta acggctcagg caaatctacc ctgctcaaat ccatagccgg
     1561 aattttgaat ccccgtaagg gcactgttgt tctcggtggg aacgagatca ccggcatcga
     1621 gctcaacagc atcatcaggc agggaatcgc ctacatcccc caggaccgag cagtttttcc
     1681 tcaaatgacc gtgagcgaga acctcgagct cggctgctgg atttttcgga aggagcaggc
     1741 gagggtccgg gcacaggtca agtgggccct tgaccgcttc cctgcgctga gggataaatc
     1801 tgattttcta gccgggagcc tctccggggg gctgcagcgg actctagaca tcgccaaggc
     1861 gcttctatcc gatccctcgc tgctgctggt ggatgaaccc tctgtcggcc tttcgccggt
     1921 catggcgcag gccatttaca gcgagcttct ttcgttgaag aaagaaggac ggacgatcct
     1981 gctggtggac caagacgtgc gctccgcgat gcagattgcc gactacgtct acgttctcga
     2041 gcttggcaaa gtgaaggccc atgcgccccg gcaagcgttc gaggggaagc tcggggaaat
     2101 tgtgcgcagc tggttaatat aagaggtgac cttgagtcct ctagatggca aatccattct
     2161 tgcgacaatc gagactatct gctcgttcgg cactcggtgg atgggagggg acggagcaga
     2221 acaaacgctg cggcatgcga ttgcggagtt caaacgcgcg ggtctcaagg tggagcttca
     2281 gccgtttcgc tacccgcact accagccgct tggggcaggg atatcagtcg aaggccacgc
     2341 gctcctctgc gagccggtcg cgctctcggg ctcgaatgcg acccccttcg aggggcagct
     2401 cgtctatggt ggcacctgct cgcacgacga gctccaggag atccgggata aagggatcga
     2461 cctggccgac gcgatcgttc tctctgacaa cctgcgatcg ttcgtggcct accccgaggt
     2521 cgaggccgcc ggcgccgccg ggttcgtctc catgacgaac cttcccgaag acacgattcg
     2581 ctgcggatgc gcgcggctgg atggaaaagt cggcaccatc cccgcagtag caatcggagg
     2641 ggctgatagg caccacatcc tcacacgcat gtcctccggc gcgagccttc gtgccgttct
     2701 ccaatcccag gggtcgatcg aggaacgaca agggttcaac gttgtcgcat ccctggcagg
     2761 agtcagtctt cccaagatcc tggtgactgc ccattacgac tgcttctgga acggagtgat
     2821 ggccatggat aactgtgctg gtgtggccat tgtccttgcg ttgagccaga cattcacccc
     2881 tatgatgctt agtggcaccg agtttgtgct gttcggtgct gaggaggtgg gctgctgggg
     2941 ggcatccggg tacattcgcc aacggagctc aagcctgggt ggcctcaagg gcaacctcaa
     3001 tttggacacc tttggttcca accggagtgg cattgaagta gggtgcacga gcgacctcga
     3061 ggccctttgc cgtagcgtag caaatcagac cggtatcgcg gtcgatgtct ggagcatccc
     3121 tccacgttcg gcgagtgatc atcagaaatt cgtggaaaac ggttatcccg cagtatggat
     3181 tgccaacggc gggaccgata cccgctacca cacgcctctc gatatcccct ctgaaatgag
     3241 tgaagaaagt ctggaaacgg ttggtcgctt agcttacagc ctagcggcta agctattggg
     3301 atcctgaggg ggagtcggcc gatctaagga ctggcactgc tgaccacctg attttgagcg
     3361 aatgtctttt atccgtattg agcagggcgc aagctgaatg aacctgcggc aaacattcct
     3421 tttagcttca atgagttaat agatgctgac gattggactg cggcgaatca aatcttcgag
     3481 gaatttcatg ttcttgatgg ttttggtcag ttaatgggaa acacaaggcg tcgccataaa
     3541 gtctgaatat gattttgcct gatagaaatc tggatagata gtttctccgg cgtatatcca
     3601 acgaaaattg gttgccgcca tcctggcgtt attgacagca tagctgcatt ccacttccat
     3661 ttccaaaccg acagatactc gatctatagt tctttagtca atgcatcgaa aaaatgcatc
     3721 gaaaaaataa gaaccagctc ggtgattccc ttttcacaac cttctgagtt atagggcaag
     3781 acaggcggcg ttcggggatc tgtgtggctc aggtgcggct atttgcctcc ggtcaggcga
     3841 gatccaggtc gcgaaaccgg ttgacctccg ggatccgttg gatatgccgc tcgtagcggc
     3901 cccgccagcc gtatttcctg agctggcggc ccaggaagac tttcagaaac ggccggaagg
     3961 cgtatatcca gatcccggtc cacagcgggc cccgcttgcg cactctctcc gacggctccc
     4021 accatcccat cacgttctgc tggtggtacc acaccaggta ttgcagctgc tccgcactga
     4081 gatggcgggt cctgacgttg gcccacatgc cgttgtagcg cgtgaagtcg tccgcgttgg
     4141 tcaccagacc ttcgtccgaa aggtattggc gcattccggt tttcgggtag ggtgtcagga
     4201 tctggcaata ggaggtgtcc gcctcgatcg ctttgaggaa tcggtagttt tcgatgatgt
     4261 cctcttcgtc gtcgtccggg aagccgaaga tcatcccggc caccaccatg atgccgtact
     4321 tgtggcaatt ggcgaccgcc tgacggg
//
LOCUS       DATMXI010000143         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_90190, whole genome shotgun sequence.
ACCESSION   DATMXI010000143 DATMXI010000000
VERSION     DATMXI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90190"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VN444_02660"
     CDS             <1..354
                     /locus_tag="VN444_02660"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HWR20746.1"
                     /translation="SMSRELLQRAIGFKMQEQACGGLTRPARLRLAALRSAAGRLNGV
                     GAQASAPTLKPGTKLLREWQGKVHDVLALEDGQFAFAGKTYRSLTTVARLITGAHWSG
                     PRFFGLKNPKVMKDG"
     gene            347..2044
                     /locus_tag="VN444_02665"
     CDS             347..2044
                     /locus_tag="VN444_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWR20747.1"
                     /translation="MADRPAVRRCAIYTRKSSDDGLEQSFNSLDAQRDACAAYIASQR
                     LEGWVLVEKQYDDGGLSGGSLNRPAIQTLLADMAKGEIDIVVVYKVDRLTRSLADFAK
                     LTELFDKNQASFVAVTQQFNTSTSMGRLTLNVLLSFAQFEREVAGERIRDKIAASKRR
                     GMWMGGHPPLGYDVKDRKLVINEQEAERVRHICTRYLELQSVRALKKDLDASDIRSKR
                     HVWKDGTAHGGVPFSRGALYLILKSPLYRGLIPHKGQIHHGEHEPIIDENLFAAVQAT
                     LDSQGPGETVRKKRASLALLKGLLFDGKGHRLQATHSNKHGRRYRYYVSAPMLRDVQS
                     YPDGLRVPAADLEKLIVDTLAARLRDKTWLTAMVGQVDEASRINQVIDAASLLAIEVE
                     QQPIMKTGILNRLIRRIGVSTKTIKLQIDMGAVLKQLLPNRTDGTLPETMEPGLEIAI
                     AGQFIRCGKQVRLVIGNDDIDQAKPDDRLIQEVVRAQQWFDDLLNGRASSITELAKRD
                     KCSAPYISRKINLAFLAPDILQSIIAGTQPRSLTQERLKKVCPLPISWQEQHAVLLA"
BASE COUNT          468 a          659 c          632 g          454 t
ORIGIN      
        1 tccatgagcc gcgagttgct gcaacgggcc atcggattca aaatgcagga gcaggcttgt
       61 ggtggtttga cccgccctgc acgcctccga ctggcagccc tccggtccgc tgcagggcga
      121 ttgaatggtg tgggcgccca agcctctgct ccgaccctca agccggggac caaactgctc
      181 cgagagtggc agggcaaggt ccatgacgtc ctggccctcg aagatggaca gtttgccttt
      241 gcgggcaaga cctatcggag cttgacgacc gtcgcccgac tgatcaccgg cgcccactgg
      301 tcggggcctc gtttcttcgg cctcaagaat cccaaggtca tgaaggatgg ctgatcgtcc
      361 cgcggtccgg cgctgcgcca tctatacccg caagtcctca gatgatgggc tggagcagag
      421 cttcaactcc ctggatgctc agcgggatgc ttgtgctgcc tatatcgcca gccagaggct
      481 ggaaggatgg gttctcgtcg agaaacagta tgatgatggg ggtctttccg gcggttccct
      541 caaccgccct gccatccaga cactactggc agacatggcc aagggcgaga tcgacattgt
      601 cgtcgtttac aaagtcgatc gcctgacccg ctccttagcc gactttgcca agctgacgga
      661 attgttcgac aagaatcaag cttccttcgt tgccgttacc cagcagttca atacgtcgac
      721 ctccatgggg cggctgaccc tcaatgtcct gctctccttc gcccagttcg agcgggaggt
      781 tgccggcgag cggatccggg acaaaattgc cgcctccaag agacggggca tgtggatggg
      841 aggccatccg ccactggggt acgacgtgaa ggaccggaag ctggtgatca acgaacaaga
      901 agccgagaga gttcggcaca tctgcacccg gtatctggaa ctccagtctg tccgggccct
      961 gaagaaggac cttgatgcat cagacatccg gtccaaacgc catgtctgga aagacggcac
     1021 cgcacatggt ggcgtcccct tttctcgggg cgccctatat ctgatcctca agagcccact
     1081 ttaccgcgga ctgatccccc acaagggcca gattcatcat ggagagcacg agccgatcat
     1141 cgacgaaaac ctgttcgccg ccgtccaggc aaccctcgat tcccaaggcc cgggcgaaac
     1201 ggtgagaaag aagcgtgcat cgcttgctct cttgaaaggc ctgctcttcg atgggaaggg
     1261 tcaccggctt caagctacac actccaataa gcatggtcgg aggtacaggt actatgtctc
     1321 agcccccatg cttcgggatg tgcagagcta tcccgatggt cttcgtgttc cggcggccga
     1381 tctggagaaa ctcatagttg ataccctcgc cgcccggctt cgggacaaga cgtggctgac
     1441 ggccatggtt ggtcaggttg acgaggcgtc acggattaat caggtgatcg atgctgcgtc
     1501 cttgcttgcc attgaggtcg aacaacagcc gattatgaag accgggattc tcaacagact
     1561 catccggcga atcggcgtca gcaccaagac gatcaagcta cagatcgata tgggcgctgt
     1621 tctcaagcaa ctcttgccga atcgcacaga tggaacactt cctgaaacaa tggagccggg
     1681 tctcgaaatt gccatcgccg gacagttcat tcgatgtggc aagcaggtcc gtctggtgat
     1741 cggcaatgat gatattgatc aggccaaacc ggacgaccgc ctcattcagg aagtcgtccg
     1801 tgcccagcag tggtttgatg atctcctgaa tggccgagcg tcgagcatca cggaactcgc
     1861 caaacgcgac aagtgctctg caccctacat cagtcgcaag atcaacctcg ccttcctggc
     1921 gcctgacatc cttcagtcga tcatcgcggg aacgcagcct cggtcactga cgcaggagcg
     1981 tctcaagaag gtctgtcccc tgccgatctc ctggcaagag cagcacgccg tcttactggc
     2041 gtaatcgtac cgacactcca ctgttgttcc gccggtcgtg tggtgttgtt gcgcattcgg
     2101 atcgcccaat ctgcttgcga cgcacataat aagcggtggg gaagggtctg gttgatgcgc
     2161 cctgttcatt gtccccaatg cagacgctgc gcgtaccata accggaattc ggg
//
LOCUS       DATMXI010000144        13361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_117191, whole genome shotgun sequence.
ACCESSION   DATMXI010000144 DATMXI010000000
VERSION     DATMXI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13361
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117191"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /locus_tag="VN444_02670"
     CDS             <1..2094
                     /locus_tag="VN444_02670"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HWR20748.1"
                     /translation="ERASTLAALALTYGLLLWYLKPSLLLKPTITGGGDTISHYVAAH
                     YLRNYLLPHGKLIGWMPGNFAGYPLFLFYFPLAFLLIAGLSFVISFPVAFKLGTIAGT
                     FGLPIASLVALRWIGCPFPIPALGAILTLPFLLNAGNSMWGGNLPSTLAGEFSHSLSL
                     ALLVLFMGALYRGVLERRYVGWCAVLFALVSLAHGYGLVMAAGFGLYFLAVESDFRGT
                     LLYLVRIYAIGGLLLGFWLIPLVWYLPYTTGFPITWEFQSILEIVPVILWPILVIGAV
                     TVGLAAWEKPVDKRWLQIVYYLTYPVVLSVTLYFVSPQLNLVDVRFLAFAQFFLVLLA
                     AAGLGRFLIRLPQSLRRAAPPIIAVLVIAWVMPQVSFIPKWIRWNYEGMEQKPWWPYY
                     RVVNSVALAGGPHSPRVVYQHSVLHDKAGTIRAFEALPLFSGRPTLEGAYMQSSPTSP
                     FVFYIQSELTETPSCPFVPYKCTTFDPERALAHLELFNVKEVIAVTDKVKAALGSSPG
                     YREEGEIGPYKIFQVRQGSGQYVVPLRHQPVLVTDGDWKRLAYDWFQKPEWLEVPLLF
                     PRPGDPAPPGAVPFHDLQQGPVKQPFSQECRVNDALGSEEVRFDTDCPGRPHLIKISY
                     HPKWRVEGADRVYLASPAFMLVYPTARQVRLVFGNRWPDYVGRAATVVGIAWLLAETL
                     VFLSRKRYSSLENPS"
     gene            2198..3556
                     /locus_tag="VN444_02675"
     CDS             2198..3556
                     /locus_tag="VN444_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR20749.1"
                     /translation="MAGRRLKVLLVNPSWDGLVSRKGRRFNRTWPPLDLLNCAALLEQ
                     DGLSVSLIDARAIPTPLDAIRDAAARHDLVFVTSSPIDRWQCPNLDLSPFLAVTHLVE
                     SGKLHVMGTHGTVAPEVLLTMTGARTLIRGEPELTVRALCEGKDPSEVPGVTYSQGDT
                     IINNPEEPVLDLTSLPMPAFHLLNLRNYRYEILGDRFALLEATRGCHYRCAFCLLEMY
                     GKGYRKKDPEQVVREVTYLVREAGARTGYFIDLEFTAVRDYVWNVCERLAAAGLPFEW
                     ACQTRADYVDEPLLTLMKRAGCRLIHFGVESGSARILAETNKRITLEQIERGIAETKQ
                     AGIDQVCFFMFGFPGETAEDMEATIAFAKRLNPTYASFHAVTPYLGTKLYEMSGSSEL
                     FPEVLSKEHNPLLLQAIADRAFREFYLRPAVLWSRLQRMDLRHWRRQAALFRHFVRPA
                     FS"
     gene            3611..4333
                     /locus_tag="VN444_02680"
     CDS             3611..4333
                     /locus_tag="VN444_02680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20750.1"
                     /translation="MTFIIPAYNEEAILAANVDRLRCYLKDREIAAYEMLLVSNGSTD
                     RTVEIARACAEGRTDLTVIELSRRGVGLAFKEGVRHARYERLISLDLDLTIDLGFIES
                     AAGLLGQVHLVIGSKQVGSQQRSWVRRLASATFIACTRTLLGLRFTDYSIGAKAYQKG
                     IIEPYLPYLADGSAYVLQLMAWRAWAGSPIAEVPVRCEDYRKSRFNLLHEGIYRFGSL
                     VPLLFQRWGRKTTPVRYGCDGP"
     gene            complement(4375..4530)
                     /locus_tag="VN444_02685"
     CDS             complement(4375..4530)
                     /locus_tag="VN444_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20751.1"
                     /translation="MPDLVDTLFLDMDAIGPEFSRLARDLISSGWVITHDESGILLAR
                     GVLKPTG"
     gene            complement(4728..5639)
                     /locus_tag="VN444_02690"
     CDS             complement(4728..5639)
                     /locus_tag="VN444_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20752.1"
                     /translation="MIMMRRGAIIVILLGAIIAFAFSRAVDAATNAELVPGSRIVFPY
                     YDLRPGFATFLYLTNVSSTPVSVALEFYNTSCSRQDAHIDLSAHDIDSLDLSEMISGN
                     ASESFLQGFVDVTTSADVLIGTAVVVNMADDWAIVYNGAPARRLAAGITPFEPYPAGL
                     FLPAFLTPGQLGGSVLADGLLIVAAPNPTRPGGKLPNTPIRASAEILLRQEQTTMSGD
                     QHRPHEMIQISSRFSGHHLILPIGAMTGPLAFPMLGWLSLTNHATDKKGVPIGLVGLY
                     IQTLVGPSSASAMAIRLSGDPSAASTP"
     gene            6000..6512
                     /gene="xrtH"
                     /locus_tag="VN444_02695"
     CDS             6000..6512
                     /gene="xrtH"
                     /locus_tag="VN444_02695"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HWR20753.1"
                     /translation="MDVRFLINKSLVRAYLTFFVCIIVFYTLLRTSIMREIIGHPLAL
                     AFAAVSGFILNLLSLKTTASGTLLQVEGFAARVDDVCTGVFVVAIYCAAVLAYPSQIK
                     EKLKGVLLGTSVIFSLNLIRVVSLMFIGRFFPSFFETAHLLIWQSLIIFAALLAWIYW
                     TERFVSAPQH"
     gene            6496..7125
                     /locus_tag="VN444_02700"
     CDS             6496..7125
                     /locus_tag="VN444_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20754.1"
                     /translation="MPPNTKALLVFAGRFLLVFFLILPLWFVITPGYNRLLASCANIL
                     LTLTEDPHVHRLVGWKYFVLITRLDSPFTEGMKVQAFTGYLTHFNLILMTGLVLAQQH
                     VAWRRRCVILAVALSVLFITHILYLVIGVKFFEQPELEAFQSTAGRVYVWGVNFYLSI
                     VAQLVPVLIWMALYRTLGEMSAEVREVDEESASGRRGKASGRRKADRVP"
     gene            complement(7300..8313)
                     /locus_tag="VN444_02705"
     CDS             complement(7300..8313)
                     /locus_tag="VN444_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR20755.1"
                     /translation="MALEGAGATCYTAAAMNYPPHLSVIIPLLNEAEGLPELYRELRQ
                     ALRPWADSHELIFVDDGSTDGSFAALEKLHAEDPRVVVLRFRRNQGKATALMAGFGRA
                     RGEILVTLDADLQDDPREIPRFLEKLEEGFDLVSGWKVERQDPIIRLVLSRGFNRVTA
                     LLTGVALHDFNCGFKCYRRDVIQEIRLHGELHRFIPALASWRGFRIAEIEVAHRARKY
                     GQSKYGSERIPRGFFDLLTVLMLTRYHLRPLHLFGTLGFLLSLAGSTVLAYLIVGWFF
                     GQWIGDRPLLSLSILTMITGLQFVFFGLLAEIIVYSSRQTDIPPVAAVLDQGDSSPPT
                     PVA"
     gene            8439..9971
                     /locus_tag="VN444_02710"
     CDS             8439..9971
                     /locus_tag="VN444_02710"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWR20756.1"
                     /translation="MTVIARSRFRLPALLLMLTVAGCAMQRPSPEEAAPDSMRLTTPE
                     TQPGQHQQIVLQMAMGAHSEAYLVWMRAGREEKDWTFLFSRSEDGGATWSGPLWSRKP
                     EQGMFIEAIKLATGSKGQVYLAWRERNAEKTDTRVLMTRSLDGGKHWDETPRQLAAGD
                     HLGPPYLQPGPDEVLYVAWLGGDESRRVLEVATSLDSGGTFTPTPIQPALAESDRGIN
                     NPRLAADEKGRLYVAWEEGTKGSRRRAGIYLSRSEDQGRTWSEPILVSRLGADSRGAH
                     VPEIAAASDGQVYLIWEQSVERTVKIEGRRRPVWPIDRLVYFNRSLDGGRTWLSEPIR
                     LGQPNPMSLNRLRSTGTQVVADGRGHVYAAFIEAEGTELWRLVVRHSADSGATWEAPT
                     ELSRTSRVKGRPITAVLAYGGEGRLWLVWQEHAFGPRYGWFVLMNRSEDHGRKWAKQS
                     VIIGEPTVGQTTDRDVLVGVGENSLILTAWDHGARVYQPIALNRSVDGGKSWSSSRLP
                     LD"
     gene            9998..11773
                     /locus_tag="VN444_02715"
     CDS             9998..11773
                     /locus_tag="VN444_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR20757.1"
                     /translation="MGKRTELKPPSAGGWLGRQSIALLILLMAVAAVYANSLDNEFLF
                     DDVETIVELHRPGAGPFDQIKALLVGGLAYRPVRSASYAMDYAISGLNPWGYHLTNIA
                     LHGLSAVFVYLIARTLFASPPPALLAALLFAVHPIQTESVTYLSGRRDVLSGLFVLAG
                     FYMFLRYRQSGRLRDLVLSILLCPLAFFTKESGIILPLLCLGYDVVSRIKKSGPDGEV
                     SGVRDVWAAASAAVREGRWFYLPAGALTGALTFYVLFLVRGTSQRTWHGGSIEMTLLT
                     MARVFLRYLVLLLFPATLNADYSYNAFPVTTSWGDPTAWLAVAIWGGLGVAWYRLLLR
                     RPPAAFGGLWLLIALLPVSQIVPHHDLMAEHFLYVPSVGFALLVAGLLEPVLNRPRPS
                     PAVAAACILALSVLAVRTVVRNANWKDELTLWRKTVQTTPESARARNNLGAAYLRQGQ
                     RTLAEEQFAAAVAIQPDLAVAHGNLGKLFLDRGDLAQAEEELERALAIQSRDTIPRLW
                     LGAVYLKQGKATEAEAQFRQALSHPRSAPYAHNNLGILLARRDQWVEAEAAFREALHL
                     MPNLREAQDNLRRLSARRERSTGNP"
     gene            11958..13115
                     /locus_tag="VN444_02720"
     CDS             11958..13115
                     /locus_tag="VN444_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20758.1"
                     /translation="MTRREFLQAASIVAGGLALSGALPKAAAGAQSPVSVPKVLDGTQ
                     TILTPLITRHARLLDDPWVLMHGVRAMGPGFTVGSERAVDLLCSRFLKTQRVAGKEYL
                     YMPVEHEGHPNACLKTLLEVGVPLSHPFTLNGRRYTVGDLVNGAKALFTFDPKTIDRD
                     NLAWTLIAFSLQTAPSQDTWTNAWGQQIRLADVVRFGLDTLDETTRQFRRAKAQGVMP
                     TEKDTIMGLTCGGTHLAYSLASCVANGHGGEEGRARLQDYLALHIWRLQADGYLMDRF
                     YHQAAPPKDASPALQRLAALYYHDARLKFYGHSFEIISYAEHHRLLAPATAEARIIEQ
                     GARTLHDAVKAIEGVDFFEFRKTNLRLFHHLVGDSCHAYHGIRMALSVNQA"
BASE COUNT         2539 a         3830 c         4087 g         2905 t
ORIGIN      
        1 gagagggcga gcacgttggc cgcccttgcg ctgacctacg ggctgctgct gtggtacctg
       61 aagccctccc tcctattgaa gccgaccatt accggcggcg gcgatacgat ttcccattat
      121 gtcgccgcac actatcttcg aaattacctt ctgcctcatg gcaaactcat cggctggatg
      181 cctggtaact ttgccgggta tcccctcttt ctcttctatt ttcccctcgc attcctgctg
      241 attgcaggat tgagttttgt tatttcattt cctgttgcgt tcaaactggg aacaatcgcc
      301 ggtacgttcg gcctaccgat cgcctcgttg gtcgcgcttc gctggatcgg ctgtccgttt
      361 cccattccgg cccttggcgc catcctgact ctcccattcc tattgaacgc aggcaacagc
      421 atgtggggcg gcaacctccc cagcacacta gccggcgaat tttcccatag cctctcgctc
      481 gcgcttctcg tgttattcat gggcgcactg tatcgaggtg ttcttgaacg acgctacgtc
      541 ggctggtgtg ctgtactctt cgccctcgtc agcctggctc atggctacgg gctggtgatg
      601 gcggcggggt ttgggctcta ctttctcgcc gtggagtctg atttccgggg cacactactg
      661 tatctggtgc gcatctatgc gattgggggg ctgctcctag gattctggtt gattccgctg
      721 gtctggtatc ttccctatac cactgggttc cccattacgt gggagtttca atccattttg
      781 gagatcgttc cggtcatact gtggccgatc ctggtcatag gggcggtcac ggtgggcttg
      841 gcagcatggg agaagcctgt cgacaagaga tggctccaaa tcgtctacta cctgacctac
      901 cctgtggtgc tgtcggttac gctgtacttt gtctctcccc aactgaacct cgtagacgtc
      961 aggttcctgg ccttcgctca gttttttctc gtcctgttgg cggcagccgg tctcggtcgt
     1021 ttcctgatca ggctgccgca gtcgctcagg cgagcggcgc ctccaatcat cgccgtgctg
     1081 gtaattgcct gggtcatgcc tcaggtatcg tttatcccaa aatggatccg ctggaactac
     1141 gaagggatgg aacagaagcc atggtggcca tactatcgtg ttgtgaattc agtagctttg
     1201 gctggaggcc cccacagccc gcgggtcgtg taccagcact cggttctgca tgacaaggcc
     1261 ggtacgatca gggcgttcga ggccttgcca ctcttctcgg ggcgtcccac gcttgaaggg
     1321 gcatacatgc agtcctcccc gacctccccg ttcgtatttt acatccagtc ggaactgacg
     1381 gaaaccccct cttgcccctt tgttccgtac aagtgcacga cctttgaccc ggaacgggcg
     1441 ctcgcccatt tagaactgtt caatgtgaaa gaagtgattg cagtcacgga taaggtcaag
     1501 gcggccctgg ggtcgagccc tgggtatcgc gaggagggcg agattggacc atacaaaatc
     1561 ttccaggttc gccagggcag cggccagtat gttgtaccgc ttcggcacca gccggtgttg
     1621 gtgaccgatg gcgactggaa acggctcgct tatgactggt tccaaaaacc ggaatggctg
     1681 gaggtgccgc ttctcttccc gcgacccggc gatccggcgc cgccgggagc cgtgccgttc
     1741 catgacctcc agcaggggcc ggtgaaacag cctttttctc aggagtgtcg ggtgaacgat
     1801 gcgctgggga gtgaagaggt ccgatttgat accgactgtc ccggtcgccc gcacctgatc
     1861 aagatctcgt atcatccgaa atggcgcgtt gagggcgccg atcgggtcta tctggcgtca
     1921 ccggcattca tgctggtcta tcccacggcc aggcaggtcc ggctcgtgtt cggcaatcgc
     1981 tggcccgatt atgttggacg ggccgctaca gtggttggga ttgcttggct ccttgcggaa
     2041 acgcttgtgt ttctttcgag gaaaagatac agttccttag aaaatccctc ctaacctccc
     2101 ttttccaaat ggaggaataa attccctctt tggcaaagag gggtaagggg agatttcaga
     2161 ataactgttt aatatgatgg gatgaggtga tctcagcatg gcgggaaggc gactgaaagt
     2221 cctgctggtc aatccaagct gggacgggct ggtgagccga aaaggacgcc ggttcaatcg
     2281 gacttggcct ccccttgacc tgctgaactg cgcggcgctg ctggaacagg atgggctgag
     2341 cgtcagtctc atcgatgccc gcgccatacc caccccgctc gacgcgattc gagacgccgc
     2401 agcccggcac gatctggtct tcgtgacctc gtcgccgatc gaccggtggc aatgccccaa
     2461 cctcgatctg agcccattcc tggctgtcac acacctggtg gaatccggta agctgcacgt
     2521 gatgggtacg catgggactg ttgcaccgga agtgctgctc accatgacgg gggcgaggac
     2581 gctcatccgt ggcgagccag agctgacggt ccgagctctc tgtgagggca aagacccttc
     2641 tgaggtaccg ggtgtcacct attcgcaagg ggacacgatc atcaacaacc cggaggagcc
     2701 ggtactcgac ctgacgagcc tcccgatgcc cgcttttcac cttctcaacc ttcgcaacta
     2761 ccgttacgaa attctcggcg ataggtttgc cctacttgaa gcaacgcgcg gttgtcacta
     2821 tcggtgcgct ttctgtctcc tcgagatgta cgggaaaggc taccgtaaga aagaccccga
     2881 gcaggttgtc cgtgaggtga cgtatctggt ccgagaagcg ggagcccgaa ccgggtactt
     2941 tatcgatctg gagtttacgg cagtgcggga ctatgtctgg aatgtgtgtg agcggctggc
     3001 agcggccggg ttaccctttg aatgggcatg tcagacgagg gccgattatg tagacgaacc
     3061 gcttctgacc ctgatgaagc gcgccggttg tcggctgatc cactttggcg ttgaaagcgg
     3121 ttcggccaga attttggcag aaacgaacaa gcgcattact cttgagcaga tcgaacgggg
     3181 aattgcggag acgaagcagg cagggatcga tcaggtctgt tttttcatgt tcggcttccc
     3241 tggtgagacc gccgaagata tggaggcgac gatcgccttt gccaagcgac tcaacccaac
     3301 ctatgcgtca tttcatgctg tgacacccta cctgggcacg aagctgtacg agatgagcgg
     3361 ctcctcggaa ctgttcccag aagtgctgag caaggagcac aatcccctcc ttcttcaggc
     3421 catcgccgac cgggccttcc gtgagtttta cctgcggcca gcggtactct ggtccaggct
     3481 tcagaggatg gatctgcgcc attggcgtcg gcaggccgca ctgttccggc actttgtgcg
     3541 accggccttt tcctaggccc tttgcgccat gcccctagag ccgcttgaga cttgccaaca
     3601 ccgggtgccc ttgacattca tcatcccggc ctataatgaa gaagccatcc ttgccgccaa
     3661 cgttgaccgt ctgagatgct acctgaagga ccgggagatt gcggcgtatg aaatgcttct
     3721 ggtgagcaac ggttcgaccg accggactgt tgagattgcc agggcatgcg cggaggggcg
     3781 caccgacctc acggtgattg agttgtcgcg gcgcggcgtc ggcttggctt tcaaagaagg
     3841 cgtccggcac gctcggtatg aacgactgat ctcgctcgac ctcgatctga cgatcgatct
     3901 tgggttcatc gagtcggccg cgggtctgtt ggggcaggtc catctggtga tcggctcgaa
     3961 acaggtcgga tcacagcagc gctcgtgggt cagacgcctg gcaagtgcga cctttattgc
     4021 gtgtacgcgt acgctgttgg gcctcagatt caccgattac tctatcggcg ccaaggcata
     4081 tcaaaaaggc atcatcgagc cctacctgcc atatctggcc gacggcagcg cctatgtgtt
     4141 gcagctcatg gcctggagag cgtgggccgg gtcgccgatc gctgaggtcc ctgttcgatg
     4201 cgaagattat cgaaagagtc ggtttaacct gcttcatgag ggtatctatc ggtttggtag
     4261 cctagttcca ctcttgttcc agagatgggg acgcaagaca actcctgtga gatacggctg
     4321 tgacggaccg tgaatgcgta agaatggacg caactgtgga agttcggccg gtcttcatcc
     4381 ggtaggcttg agaactcccc gtgccagcaa gattcccgat tcatcgtgag taatgaccca
     4441 ccccgacgag ataaggtcgc gcgccagccg gctgaattcc ggcccgatgg cgtccatatc
     4501 gaggaatagc gtatcaacca ggtccggcac gggcaggttc tcgccgttga ttccccagta
     4561 cgccgatttc cacggatggg aacataaaga tccaattccg accgactgtg acacggcgag
     4621 agtggaaaag tcgtatccac gtgctgacgg agcttgcgct ttgcgaccct atggagactg
     4681 ggcgccgcca tggtttgtgg aatgcggcgt aaactgtgtt cacgctgcta tggagtcgag
     4741 gcagcggatg gatcgccgga tagtcgaatg gccattgccg aggcgctact tggtccgacc
     4801 aacgtctgga tgtacagacc aaccagcccg atagggaccc ctttcttatc cgtcgcatga
     4861 ttcgtgaggg acagccatcc caacatcgga aaggctaagg gaccggtcat tgcccctatc
     4921 ggcagtatga gatgatgccc actgaacctg gaagagattt ggatcatctc atgaggccgg
     4981 tgttggtccc cgctcatggt ggtttgctcc tgtcgcagga ggatttcggc cgaagcccgg
     5041 atcggcgtat tcggcagctt gccgcccgga cgggtgggat tgggcgcggc gacgatcagc
     5101 aggccatcgg ctagcacact gccacccagt tgtccagggg tcaggaacgc gggtaaaaag
     5161 aggccggccg ggtagggttc gaatggcgtt atgccggctg ctagtcggcg agccggcgcc
     5221 ccgttataca cgatcgccca gtcgtctgcc atattgacga caactgccgt gccgatcaga
     5281 acgtccgcgc ttgtggtgac atcgacgaag ccctgcaaaa agctctcgga tgcgttgccg
     5341 gagatcattt cggataggtc gagtgaatcg atatcgtggg ctgacaggtc tatgtgggcg
     5401 tcctgacggc tacatgatgt attgtaaaac tccaaggcga ccgacacggg ggttgagctg
     5461 acgttggtca ggtacaggaa ggtcgcaaat ccaggtcgca ggtcatagta ggggaagacg
     5521 atcctcgacc ccggaaccag ctcggcattc gtggcggcgt cgaccgcccg cgagaacgca
     5581 aaggcgatga tcgcacccaa gaggatgacg ataatggcac cacgacgcat cataatcatc
     5641 tcgacaacgc ctcctccaat ccagccgacc ttcgccatca accgggttgc ggagatgcag
     5701 acaacggctg atcataccat agcagtgccg ttgcaaccct gtcaaggata acgaccgaga
     5761 ttatgttgtc agtagcaagt cgagatgtcc ggttgttaca gaaaatgaaa tgtccggtta
     5821 gtcatggtgt ccgtcgctcc gatcctgtac gacgacgcag gcgccgggta ttgacaacgg
     5881 gcttcatcct gttgtattga tgtagctgga ttgctgagcc gtatgtccgc gtaatgtgat
     5941 tctgaggttc catgagggag taggaagagc aatatggcgt ggaggtgagc aaggcggcga
     6001 tggacgtgcg atttcttatc aataagtcgt tggtcagggc gtacctcacc ttttttgtgt
     6061 gcatcatcgt attttacacg ctcctacgaa caagcatcat gcgggagatc atcgggcatc
     6121 cgctggcgct ggcgtttgcc gcggtcagcg ggttcatcct gaacctcctt tccctcaaga
     6181 cgacggcttc gggaaccctg ctccaggttg aggggtttgc ggcccgggtt gatgatgtat
     6241 gcaccggtgt ctttgtcgtc gcgatctact gtgccgccgt cctggcctat ccaagtcaga
     6301 taaaagagaa actcaaaggt gtcttgctcg ggacatcagt catcttctcc ctcaatctca
     6361 tccgggtggt gagcctcatg tttatcggcc gcttttttcc gtcattcttc gagacggccc
     6421 atctgttgat ctggcaatcg ttgatcatct ttgccgccct cctagcctgg atctactgga
     6481 cggagcggtt tgtcagtgcc ccccaacact aaagcgctgc tggtttttgc cggccgcttt
     6541 ctgctggtct tcttcctcat ccttcccctg tggtttgtca tcacccctgg ctacaaccgc
     6601 ctccttgctt cctgtgccaa tattctgctt actctcacag aggaccctca tgttcatcgc
     6661 cttgtaggtt ggaaatactt cgttctgatc acgcgcttgg atagcccttt tacggaaggg
     6721 atgaaagtcc aggcattcac agggtatctg acccacttca atctgatcct catgacaggc
     6781 ctcgtgttgg cccagcagca cgttgcgtgg aggcgtcggt gtgtaatcct ggcggttgcg
     6841 ttgagtgttc tgtttattac acacattcta taccttgtga ttggggtaaa atttttcgag
     6901 cagccggaac ttgaagcctt ccagagcact gcaggtcgcg tctatgtctg gggggttaat
     6961 ttctatcttt cgattgtggc tcagttggtg cctgtgttga tttggatggc cctgtatcgg
     7021 actttgggcg aaatgtcagc cgaggtgcgt gaagtcgatg aggagagcgc ttccggacgg
     7081 agaggaaaag cgagcgggag gcgcaaggca gatcgcgttc cgtgaaatga cgaagcatcc
     7141 aacccacgtt cgcgtcactg cgagcgacca tcgggagcgc ggcaatctca ccgtagttgt
     7201 cctgaacgac tgtgagattg cttcggcttc gcctcgcaat gaccgctgag ggagactttc
     7261 tggataatga cgatcaaaag aacacgtgat gctgcgagct caggcgacgg gagttggagg
     7321 ggaggagtct ccctgatcga ggacggcggc gactgggggg atgtcggttt gcctgcttga
     7381 gtagacgata atctcggcca ggagtccgaa gaagacgaac tgcaggccgg tgatcatcgt
     7441 cagaatgctg aggctgagga gggggcggtc accgatccat tgcccgaaga accagccgac
     7501 aatcagatac gccagaacgg tggaacctgc caggctgagc aagaatccta aggtcccgaa
     7561 gaggtgaagc ggcctcaggt ggtagcgggt cagcatcaag acggtcagca ggtcgaagaa
     7621 gccgcgaggg atccgttcgg agccgtattt cgactggccg tatttccgcg cccggtgtgc
     7681 gacctcgatc tccgctatcc tgaaacccct ccaactcgcc aacgccggaa tgaaccggtg
     7741 cagctccccg tggagcctga tctcctggat gacatcccgc cggtagcatt tgaacccgca
     7801 attaaaatcg tgcagcgcga cccctgtgag cagcgccgtc acacggttga agcctcgcga
     7861 aagtaccagc ctgatgatgg ggtcctggcg ttcgaccttc catcctgaca ccagatcgaa
     7921 cccttcttcc agcttttcaa gaaatcgcgg gatctcccgc ggatcatcct gcaggtcggc
     7981 gtcaagcgta accaggattt cgcctctcgc ccggccaaac cccgccatca acgcggttgc
     8041 ctttccctga ttccggcgaa agcgaaggac gaccacccgt gggtcttctg catgaagctt
     8101 ttcgagggcg gcaaacgagc catcggtgct gccgtcatcc acaaagatca gctcatggct
     8161 gtcggcccat gggcggaggg cctgtcggag ctcccgatag agttccggca aaccttcggc
     8221 ctcgttcaag agggggatga tgaccgacag atgtggcgga tagttcattg cggccgcagt
     8281 atagcaggtc gcccctgccc cctcaagcgc catttccaac tgaccgttgg tgttgacagc
     8341 aggcggcgtt ttgtgagaac gtataaagct tttacgtgtg cgcgctcgat ctataatcgg
     8401 gcggcagtgg ccttttacat caatggaggg gactctacat gactgtgatt gctcgaagcc
     8461 ggtttcgcct accggcgctc ctgctgatgt tgactgtagc agggtgtgcc atgcagcggc
     8521 cgagcccgga agaggcggcc cctgatagca tgcgactcac gacgccagag acacagcctg
     8581 ggcagcatca gcaaatagtg ctccagatgg ccatgggggc gcatagcgag gcatatctgg
     8641 tctggatgag ggccggacgg gaggaaaagg actggacatt cctattctcg cgttccgagg
     8701 atggcggcgc cacctggtca ggtccgctgt ggtcccgaaa accggaacag gggatgttca
     8761 ttgaagcgat caagctggcc accgggtcca aaggccaggt gtatctggcc tggcgggaac
     8821 ggaatgccga gaagaccgat acgcgcgttt tgatgaccag gtccctggat ggcgggaagc
     8881 actgggatga gacgcctcgt caactggctg ccggggacca tcttggacct ccttacctgc
     8941 agccggggcc ggatgaggtc ctgtacgtgg cctggctggg tggagatgag tcccgacggg
     9001 ttttggaggt ggctacctcc ctggattccg gcgggacctt tacgccgacg ccgatccagc
     9061 cggctcttgc ggagtcggac agaggaatca acaatccgag actggcagcc gatgaaaaag
     9121 gccgcctgta tgtcgcctgg gaagagggaa cgaagggatc gaggcgacgc gccggaatct
     9181 acctgagtcg ctcggaagac cagggccgaa cctggtcaga gccgatcctt gtcagccgcc
     9241 ttggggcgga ttctcgtggc gcccatgtgc cggaaatcgc cgcggcatcc gacggacagg
     9301 tctatctgat ctgggaacag tcggtagagc gaaccgtcaa aatagagggc cgacgtcgac
     9361 cggtgtggcc gatagaccgg ttggtctact ttaaccgttc tctcgacggc ggtcggacct
     9421 ggttaagtga gccgatccgc ctgggtcagc ccaacccgat gtcactgaac cgactcagga
     9481 gtacgggcac tcaggttgtc gccgacggac gcgggcatgt ctatgccgct ttcatagagg
     9541 cggaggggac cgagctgtgg cgtctggtgg tgcgtcattc cgccgattcc ggggcgacgt
     9601 gggaagcgcc caccgaatta agccggacga gtcgggtcaa gggccgaccc atcactgccg
     9661 tgctggcgta tggcggtgag ggccgcctct ggcttgtctg gcaggaacac gcctttggcc
     9721 ccaggtacgg gtggttcgtc ctgatgaatc ggtccgagga ccacggccga aagtgggcca
     9781 agcagtccgt catcataggc gagccgacgg tcggacagac gaccgataga gacgtgttgg
     9841 ttggcgtggg cgaaaacagc cttatcctga cggcctggga tcatggcgcg cgagtgtacc
     9901 agccgatcgc gctgaaccgg tccgttgacg gcgggaagag ctggtcgtcg agtcgccttc
     9961 cgctcgactg accgccgcag gggaaggatg agggatagtg ggtaagcgga ccgagctgaa
    10021 gccgccgtcc gctggaggct ggctcggccg acagtcgatc gccctgctta tcctgcttat
    10081 ggcggtcgcc gctgtctacg ccaacagcct cgacaacgaa ttcctgttcg atgacgtcga
    10141 aacgattgtc gaactccacc gccccggcgc cggaccgttc gatcagatca aggcgttgct
    10201 ggtcggcgga ttggcgtatc gtcctgtcag aagcgcctcg tacgccatgg attacgccat
    10261 ctccggcctc aatccatggg ggtaccacct caccaacatc gcgctgcacg gcctctcggc
    10321 cgtcttcgtc tacctgatcg cccggacact gttcgcctcg ccgcctccgg ccctccttgc
    10381 cgcgctactc ttcgcggtcc acccgatcca gaccgaatcg gtgacctacc tgtccggacg
    10441 ccgggatgtc ctgtcgggct tgtttgtcct ggccggattt tatatgttcc tgcgctaccg
    10501 tcagtccggt cggttgcggg atcttgtgct gtcgattctg ttgtgtccgc tggccttctt
    10561 caccaaagag agcgggatta tcctgccgct gctgtgtctc ggctatgatg tcgtaagccg
    10621 catcaagaag tcggggccgg atggggaggt ctccggcgtt cgggacgtct gggcggcggc
    10681 gtctgccgca gtccgggagg gacgatggtt ttacctgcct gccggcgcct tgaccggagc
    10741 cctgaccttc tatgtgctgt ttcttgtgcg ggggacctcg caacggacct ggcatggcgg
    10801 cagcatcgag atgacgctgt tgacgatggc ccgcgtgttc ctgcgctacc tcgtcctcct
    10861 gctgttcccc gcgacgctga atgccgacta ctcgtataac gcttttcccg tgaccacctc
    10921 ctggggcgac ccgaccgctt ggttggcggt ggcgatctgg ggagggctgg gagtggcctg
    10981 gtaccgcctg ctgctccgac ggccgccggc ggcattcggc ggcctctggt tgctaatcgc
    11041 cttgctgccc gtctctcaga ttgtccccca ccatgatctg atggccgagc atttcctgta
    11101 tgtcccttcc gtgggcttcg cgttactggt cgcggggttg ctcgagccgg tactgaaccg
    11161 accccggcca tcccccgctg tcgcggccgc ctgcattctg gcgctcagcg tcctcgccgt
    11221 ccgtacggtg gttcgcaacg caaactggaa agacgagctg accctgtggc gcaaaacggt
    11281 acagacgact cccgagtcag ccagggcgcg gaacaacctg ggggcggcct atctgcgcca
    11341 gggtcagcgc acgctggcgg aagagcagtt tgctgccgcc gtggcgatcc aaccggacct
    11401 cgcagtggcc cacggcaact tgggcaaact attcctggat cgtggtgatc tggcccaagc
    11461 tgaggaagag ttggagcgag cgctcgccat acaatcgcgg gacactatcc cgcgcctgtg
    11521 gttgggagcg gtctacctga agcagggtaa ggcgaccgag gccgaggcgc agtttcggca
    11581 ggcgctcagc catccgagat cggcccccta cgcccacaac aaccttggca tcctgttggc
    11641 tcgacgtgac caatgggtcg aggccgaggc ggcgtttcga gaagcgttgc acctcatgcc
    11701 gaaccttcgg gaggcgcaag acaaccttcg ccgcctgtcg gcccgccggg agcgctcgac
    11761 tggaaatcct tgacacgccg cgcataatcc gatacaaagt aggctgcctt gcactctcct
    11821 gctcgcgtgg ttcgacttgc caaccgttcg tcctgaactt gtccaaggag acggttggcc
    11881 tgctcaggca ggctcacgag ccaaacggct ccgtacgcaa ggttgaagag aggaaataca
    11941 cctgaatgtg tatgtgtatg acgaggcgtg agttcctgca ggccgcatcg atcgtggccg
    12001 gaggactcgc gttgtccgga gccctgccga aggcggcggc cggtgcgcag tcaccggtgt
    12061 ccgttccaaa ggtcctggat gggacgcaga cgatcctcac ccccctcatc acgcggcacg
    12121 cgcggcttct ggatgatccc tgggtgctga tgcacggggt ccgggccatg ggaccgggct
    12181 ttaccgtggg cagcgagcgt gccgtcgatc tgctctgctc gcgcttcctc aagacacagc
    12241 gagtggcggg caaggagtat ctgtacatgc cggtggagca tgaggggcat cccaacgcct
    12301 gtctcaagac gctcctggag gtcggcgtcc cactgagtca cccctttacg ctgaacggcc
    12361 gccgctacac cgtcggcgac ctggtgaacg gcgcgaaggc gctgttcacg tttgatccga
    12421 agaccattga tcgcgacaac ctggcctgga cgctgatcgc cttttcgttg cagactgctc
    12481 catcccagga tacctggacg aacgcctggg ggcagcagat tcgattggcc gatgtggttc
    12541 ggttcggcct ggacacgctg gacgagacga cgcgacagtt ccgacgcgcg aaggcgcagg
    12601 gggtcatgcc gaccgagaaa gacaccatca tggggttgac ctgcggcggc acgcatctgg
    12661 cctatagcct ggcctcctgc gtggcgaatg ggcatggcgg tgaagagggg cgggcgcgcc
    12721 tgcaagacta tctggctctg cacatctggc ggttacaggc tgacggctac ctgatggacc
    12781 ggttttatca ccaggcggca ccccctaagg atgcctctcc ggccttgcag aggcttgccg
    12841 cactctacta tcacgacgcg aggctcaaat tctatggcca cagcttcgag attatcagct
    12901 atgccgagca tcatcgcctc ttagcccctg ccaccgcgga ggcgcgtatc atcgaacagg
    12961 gggcgaggac gctgcacgac gcggtcaagg cgattgaggg ggtcgatttc ttcgagttca
    13021 ggaagaccaa cctacggctg tttcaccacc tggtcggcga ttcgtgccat gcctatcatg
    13081 gcattcgcat ggcccttagt gtgaaccagg cgtaggaggc ggcgtgctgc gtccagtccg
    13141 cagtgcaact caaaagtgga ggttggcaac ccgatcttta tcatttgtcc gtcattgcga
    13201 gagaccgaag ggagcgcggc aacccaaccg ttctgcttcg ctggtcttga agaacggtga
    13261 gattgcttcg gtcgcgccgc tcctcgcaat gacggcgcgg gagactttcg aagcgatatt
    13321 tgcacaggca tagattggaa tgagtcgaat cggtgcggag g
//
LOCUS       DATMXI010000145         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_197833, whole genome shotgun sequence.
ACCESSION   DATMXI010000145 DATMXI010000000
VERSION     DATMXI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197833"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VN444_02725"
     CDS             <1..1396
                     /locus_tag="VN444_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWR20759.1"
                     /translation="LKILPGMQAAFVDLGLAKDAFLYVRDIFEDVEEYEQMLIIGEEG
                     EPGEPLSEEPRPSFSRGRRPQASIEEILKEGQEIVAQVAREPLGTKGARITSHITLPG
                     RHLVYMPTEQHVGVSRKIEDEAERSRLKQIIEEINPQREGVIVRTAGIGKERAEIEAD
                     LEFLRSLWKKIKDKAETLTAPAVVQKDLDLIFRIFRDLFTKEVVRLVVDSPAEYERCL
                     EYAESLHPDLKSHLFLYTEDEPIFKSFGIEREIEKALRHKAWLKSGGYIVLEETEALV
                     SIDVNTGKYVGKHDFEETVLKTNLEAAREIARQVRLRDLGGIIIIDFIDMARQESRDR
                     VLQDLKEALKPDRSPTNVSLLSELGLVEMTRKRVRQGLNKALSASCPACGGLGYVRST
                     PSIAHQVLREVEWRLFSKRIPMIRIRAHPDLIDWFRAEDGEVIEALQQTYGGEIILVP
                     EESLAPGKYQLLEG"
     gene            1852..>2182
                     /locus_tag="VN444_02730"
     CDS             1852..>2182
                     /locus_tag="VN444_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HWR20760.1"
                     /translation="MQAKTYLVGHRERLRARYRLNGEAALEDYELLELLLTYAIPRHD
                     TKLLAKKLLERFGTLARVFEAEPAALEAIDGIGPQAATLISLIRPLATRFVTQAPKAK
                     TVLRSTAD"
BASE COUNT          475 a          645 c          651 g          411 t
ORIGIN      
        1 gctaaaaatc cttcctggga tgcaggcggc cttcgtggat ttgggcttgg cgaaggatgc
       61 ctttctgtac gtccgggaca ttttcgagga cgtggaggag tatgagcaga tgctgatcat
      121 cggggaggaa ggtgagcctg gagaacccct ctccgaggag ccaaggccca gtttctctcg
      181 aggtcgtcgc ccacaggcca gcatcgagga aatcctgaag gagggccagg agattgtcgc
      241 gcaggtagcc cgtgagcccc tcgggaccaa gggcgctcgt atcacctccc acatcaccct
      301 ccccggtcgc cacctggtct atatgcccac cgagcagcac gtcggggttt cgcggaagat
      361 cgaggacgag gcggaacgat cccggctgaa gcagatcatc gaagagatta accctcaacg
      421 ggagggggtc atcgtcagga ctgctgggat cggcaaggaa agagcggaga tcgaggccga
      481 cctcgaattt cttcggtccc tctggaaaaa gatcaaagac aaggccgaaa cactcaccgc
      541 cccggcagtg gtccagaagg acctggacct gatcttcagg attttccgcg acctcttcac
      601 gaaggaggtg gtccgcctgg tggtagacag tccggcggag tacgagcggt gcctggagta
      661 tgccgagtca ctgcatcccg atctgaagtc gcatctattt ctgtacaccg aggatgagcc
      721 gatcttcaag tcgttcggca ttgaacgaga gatcgagaag gcgctgcgac acaaggcgtg
      781 gctcaagtcg gggggctaca tcgtgttgga ggagacggag gcgctcgtct ccatcgatgt
      841 caacaccgga aagtatgtcg gtaaacacga ttttgaagag acggtcctca agaccaacct
      901 ggaagcggcc cgggagatcg cgcgccaggt gcgcttacga gacctgggtg gaatcattat
      961 cattgacttc atcgacatgg ctcggcagga gagccgggat cgggtgctcc aggacctgaa
     1021 ggaggcgctg aagcccgacc gctcccccac caacgtctcg ctcctgtcag agctgggcct
     1081 ggtcgagatg acgcggaagc gggtccgcca agggttgaat aaagccctga gcgcttcctg
     1141 ccccgcgtgc ggcggtcttg gctacgtccg gtcaaccccc tccatcgccc atcaagtcct
     1201 tcgagaggta gagtggcgac tgttcagcaa gcggatcccc atgatccgga tccgtgccca
     1261 tcccgacctg atcgactggt tccgagctga ggatggtgag gtaatcgaag cgctccagca
     1321 gacctacggc ggagagatta tcctggttcc ggaagaatcg ttggctccag ggaaatacca
     1381 gttgctggag gggtaggcgc cagactccat aacgcttccc ccccttgcac gcgttcccgt
     1441 cggattcttt gagaagcccc gcagaccaca acatgaagcg cctcacgtta tccaatagcc
     1501 gcctgggatt gtataccggg agcccccgtt gcttctggct ggacatcaac gccaggacca
     1561 ggcgcccaga aactatcttc ccgagcctcc ctggtgggct ggaccttcag ttcaagcgat
     1621 actttgatca gttcatggac cgtagcgagc tgccccctgc ttctactacc cgctggacta
     1681 caaaacccgt gggtatgccc caaaggccgg cacccatgca ttctaccaaa accagatgaa
     1741 cctctatacg ctactgctgg aaggtaacgg ccacaaaact aaacgtctcg cgtatctgat
     1801 ctactatcat ccggtcgagg tgcaagcgca cggcctcatt cagttccagt catgcaggcc
     1861 aagacctatt tagtggggca tcgggagcgg ctgcgggctc gctaccggct caatggcgag
     1921 gcggcgctcg aagattacga gctgctggaa ctcctcctga cgtatgccat tcccaggcac
     1981 gacaccaaac tgctcgctaa gaagctcctg gaacgattcg ggacgctggc ccgtgtcttc
     2041 gaggctgagc cggcagccct ggaagcaata gacgggatcg ggccccaggc ggctacgctg
     2101 atttcgctga ttcgacccct cgcgacgcgc tttgtcacac aggccccaaa ggcgaaaacg
     2161 gtgctcaggt ccaccgcgga tg
//
LOCUS       DATMXI010000146         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_69382, whole genome shotgun sequence.
ACCESSION   DATMXI010000146 DATMXI010000000
VERSION     DATMXI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69382"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="lpxI"
                     /locus_tag="VN444_02735"
     CDS             complement(<1..700)
                     /gene="lpxI"
                     /locus_tag="VN444_02735"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HWR20761.1"
                     /translation="MECLGIIAGGGPLPVVAAREARMQGFKVVAVAVEEAASADLANE
                     VDAICWVGAGQLGRLIAALKHEKVTNAVMLGKIPLDLLFSRVKTDLDGLLFYLKLKDR
                     RGDTILASVGEVLKQKGITLHDCRRFLSSIVLRKGLLTIRAPSSLEHQDISFGRELAR
                     AIAQLRIGQTVVVKRRTVLAVEAIEGTDAAIRRGGKLGNGGVVVIKVGRPDQDMRFDL
                     PVIGPETVVALEDAG"
     gene            complement(789..1565)
                     /gene="lpxA"
                     /locus_tag="VN444_02740"
     CDS             complement(789..1565)
                     /gene="lpxA"
                     /locus_tag="VN444_02740"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWR20762.1"
                     /translation="MIQIHPSAVVAPEAAFGPDCSVGAFSVIGPDVTVGSGTAIGSHT
                     LIEGITEIGERCQIFSHVVLGAAPQIFHDRSEMTRLVIGDETVIREFASVHRGSSKGR
                     GATVLGRRNYIMAYAHIAHDCLLRDDVVVASQAGLAGHVEVEDRAVIGGQAGIHQFVR
                     IGQYAMVGACSAVSQDIPPFVKAQGDRAKCFGLNTVGLRRHGVCQEAILRLKQAYRLL
                     FLSNLNTSQALERIASEVSSCPEIDHLIHFIKLSTRGIAR"
     gene            complement(1562..2044)
                     /gene="fabZ"
                     /locus_tag="VN444_02745"
     CDS             complement(1562..2044)
                     /gene="fabZ"
                     /locus_tag="VN444_02745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWR20763.1"
                     /translation="MIDCRQIQEMLPHRYPFLLVDRILEIEPGKRVVGLKNVTINEAF
                     FQGHFPGQPIMPGVLVIEAMAQVGGILLMRTLNVSAEKKLLYLAGIDRAKFRRPVLPG
                     DQVRFEVELIQLRSRTCRMRASALVDGKLAVEAELSCIVVDRESPNLPPGVPVAMGEQ
                     "
     gene            complement(2041..>2622)
                     /locus_tag="VN444_02750"
     CDS             complement(2041..>2622)
                     /locus_tag="VN444_02750"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWR20764.1"
                     /translation="IIHSGAVIGSDGFGYLRDGQGVRIKIPQVGRVILEDDVEIGANV
                     TIDRATMGATRIKRGTKIDNLVQIAHNVVVGADTVIAAMSGISGSTTIGDRVTLAGQV
                     GIADHVEIGDDVTVGAQAGVTKSLPPGGVFLGFPAVPHLAFKRSVAAVNRLPHILNTL
                     KRIEARLALLEGAASERRGESQPSHPAPDGGPQ"
BASE COUNT          559 a          865 c          677 g          521 t
ORIGIN      
        1 cgcccgcatc ttcgagggcc accacagtct ccggtccgat aaccggaaga tcgaagcgca
       61 tatcctggtc gggacgtcca accttgatga ccaccacccc cccattccca agtttgccgc
      121 cgcggcgaat agcggcatca gttccctcga tcgcctccac tgcgaggaca gttcggcgtt
      181 tgaccacgac agtctgaccg attcgcaatt gcgcgatggc cctggcaagc tctcttccaa
      241 agctgatatc ttgatgctcc aaggagcttg gcgcccgtat cgtcagcagt cctttccgca
      301 atacgatcga agaaagaaac cgccgacaat catgcagcgt gatccctttc tgcttcaaca
      361 cctcaccgac actcgcgagg atcgtgtcac cgcgtcggtc tttgagcttc agatagaaca
      421 gcaggccatc cagatcggtc ttcacgcgcg agaagaggag gtcgagcggg atcttcccaa
      481 gcatcacagc attcgtcacc ttctcatgct tcagcgccgc gatcaaccgg ccaagctgtc
      541 cggctcccac ccaacagatc gcatccactt cattggccag atcggcagag gcggcctctt
      601 cgacggccac cgccacgacc ttgaacccct gcatgcgtgc ttcccgtgcc gctacgaccg
      661 gaagcggccc tccgccggct atgattccaa ggcattccat tgcaatccga ttcgcggtgt
      721 attgcgttta ttgcgtgcgg cgttcattgc gtgcggcgta acgctgcaca ccacacactg
      781 catgcctttc aacgcgcgat ccctctcgtc gataatttga tgaagtgaat caggtgatcg
      841 atctctggac aggaactcac ctccgatgca atcctctcca gagcctgaga cgtattaagg
      901 ttggacagga agagtaatcg atacgcttgc ttgagacgaa ggatcgcctc ttgacagacg
      961 ccatggcggc gaagaccaac tgtattcaaa ccgaagcact tggcccgatc cccctgcgcc
     1021 ttgacgaagg gtgggatgtc ctgcgaaacc gccgagcagg cgccaaccat ggcgtattgt
     1081 ccgatacgga cgaactgatg gatccctgcc tgcccaccga taacagctcg gtcttcgacc
     1141 tcgacgtggc cggccaaccc agcttggctg gcaaccacca cgtcatcacg taagaggcaa
     1201 tcgtgtgcga tgtgcgcata agccatgatg tagttccggc gccccagcac cgtcgcaccc
     1261 ctgcctttcg atgatccgcg atgaaccgat gcgaactccc ggatgactgt ctcatcaccg
     1321 atcaccagcc gggtcatctc acttcgatca tgaaagatct gaggcgcggc gccaaggact
     1381 acgtgagaga agatctggca ccgttccccg atctctgtga tcccctcgat gagggtatgc
     1441 gaaccgatgg cggtcccgga ccccacggtg acatcagggc cgatgaccga aaaggcgccg
     1501 acgctacagt ctggtccaaa cgcggcctct ggcgcaacaa ccgctgacgg atgaatctgt
     1561 atcattgttc ccccatcgcc actggaacgc ctggaggaag gttcggagat tcccggtcaa
     1621 cgacaatgca cgacaactca gcctctacag ccagcttacc atccaccaag gcgctggctc
     1681 gcattcgaca ggtgcggctc ctgagttgga tcagctcgac ctcgaatctc acctgatccc
     1741 caggcagcac aggcctgcga aacttggccc ggtcgatgcc ggcaagatac aggagcttct
     1801 tttcggcgct cacgttcagt gtccgcatca acaggatccc tccgacctgc gccatcgcct
     1861 cgatcaccag cactcccggc ataatcggct gccctggaaa gtgaccctga aagaatgcct
     1921 cgttaatggt cacgttcttg agccctacca ccctcttccc aggctcgatc tcgagaatcc
     1981 tatcgaccag aaggaaggga taccggtggg gaagcatctc ctggatctgt cgacaatcga
     2041 tcactggggt cccccatcag gagccggatg agaaggctgc gactcccctc tccgttctga
     2101 agcggcgccc tctaacaatg ccaggcgagc ctcaatgcgc ttaagggtat tcaggatgtg
     2161 ggggagccga ttcaccgcgg cgacgctgcg cttgaatgcg agatgcggca cggcgggaaa
     2221 acccaggaag acgcctccag gcggaaggct cttggtcact ccagcctgcg ctcccacggt
     2281 gacatcatcg ccgatctcga cgtgatcggc aattccaacc tggccggcca gcgtcacccg
     2341 atccccgatc gtggtgctgc ctgagatgcc gctcatggcc gcgatcaccg tatctgctcc
     2401 aacgacaaca ttatgtgcaa tctggaccag attatcgatc ttggtcccac gcttgatccg
     2461 cgtcgccccc attgtcgctc gatcgattgt cacgttggcg ccgatctcca catcatcctc
     2521 gagtatcacc cgtcccacct gtggaatctt gatacggacc ccctgtccgt ccctcagata
     2581 cccgaagcca tcgctaccga tcacagcgcc gctgtggata at
//
LOCUS       DATMXI010000147         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_47658, whole genome shotgun sequence.
ACCESSION   DATMXI010000147 DATMXI010000000
VERSION     DATMXI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47658"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VN444_02755"
     CDS             complement(<1..319)
                     /locus_tag="VN444_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20765.1"
                     /translation="MGEPEKRELPSGMLSDLKGRRGAIIAGWLVYAFASLGFGAASQP
                     WHIWALIGFYGLYFGLAEGRERALTADLVSPDRQAYGLWPLSLLYRDRRLGRPFSPPA
                     PHLA"
     gene            676..1593
                     /locus_tag="VN444_02760"
     CDS             676..1593
                     /locus_tag="VN444_02760"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HWR20766.1"
                     /translation="MSKKHPVIAVAGSSGAGTTFVKRAFENIFRREGITSAIIEGDSF
                     HSVTRTQFKERSAVEHNFSHFGPGANDFHALEALFKSYGEAGVGKKRHYLHNAEEAAF
                     HCKRMADAGVTCNAGSGEFTPWEEIESNTDVLFYEGLHGLVKDVSPDQKYGGYDVAQH
                     VDLGIGVVPSVNLEWIQKIHRDHAERGYSPEATVDTIMRRMPDYINHLAPQFSRTHVN
                     FQRVPTVDTSNPFIARDIPSADESFVVIRFVDPKRFGVDFPILLAMINGSFISRRNTI
                     VVPGGKMVLAMELILAPIIHIMIENKLKA"
     gene            complement(2047..3225)
                     /locus_tag="VN444_02765"
     CDS             complement(2047..3225)
                     /locus_tag="VN444_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR20767.1"
                     /translation="MFGLSRNVVALGLVSLLTDVSAEMIYPLLPLFLTTVLGAGQTFV
                     GLVEGIAESAASLTKLFSGWLSDRLGRRKGLVVVGYTLSTLTRPLVAFALIPWHVLMV
                     RFADRLGKGLRTSPRDALIAGSTDLAIRGRAYGFHRSMDHLGAVAGPALAYVLLLLLG
                     GGQLRTIFLLAAIPGILSVLILILKVSDIQVNPTAAPKATSGTGRMNRQLTRFLFVVT
                     LFTLGNSSDAFLLLRAQEVGIAVAHLPLLWMYFHVVKAATGMPGGMLSDLRGRRGAII
                     AGWLVYAVAYLGFGAASQPWHIWVLLGFYGLYFGLTEGGERALIADLVPPDRQASAFG
                     LFHSCIGIAALPSSLLMGYLYQSFGAWPAFTVGAALAGLSALLLFILFRPPSPQTSRV
                     "
     gene            complement(3239..3745)
                     /locus_tag="VN444_02770"
     CDS             complement(3239..3745)
                     /locus_tag="VN444_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWR20768.1"
                     /translation="MNFKSLPIGNSAPVIVNAVVEIPSGSSNKYEYDVILGVFRLDRV
                     LYSPVHYPADYGFIPGTLGQDGDPLDVLILISQPTFPGCLVEARPVGMLDMVDDKGID
                     EKILAVAHGDPRYQRIEDLAGVEPHVLKEIEHFFNVYKNLEGRTSLTSGWLGRGAAHE
                     RIETYRVR"
     gene            complement(3783..4487)
                     /locus_tag="VN444_02775"
     CDS             complement(3783..4487)
                     /locus_tag="VN444_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWR20769.1"
                     /translation="MRILVLSDLHANLAALEAATAEEFDRLICLGDLVGYGPSPNEVI
                     RFIRERAFVAIRGNHDNALALDVECRCSPANKALAEATLAYHRTLLSSEDLDWLRALP
                     GSVEFTLDGYRFAAFHATPRDPLYGYEITPELPDDQLREQIEGVKADFILLGHTHLPM
                     VRGIGSRVIVNPGSVGQPRDGNPECSYAIIEDGVVELRRAKYDLSGTVDALRKLPLSG
                     ELIDQLISILEQSGPQ"
     gene            4659..>4916
                     /locus_tag="VN444_02780"
     CDS             4659..>4916
                     /locus_tag="VN444_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWR20770.1"
                     /translation="MHSFRQVIVDEILPAVSKPSRYIGMEWNAVKKDPANAKVKVALA
                     FPDTYEIGMSHLGLKILYQVLNRHPEFMAERAYSPWVDAERL"
BASE COUNT         1095 a         1417 c         1420 g          984 t
ORIGIN      
        1 gagcaagatg aggagcagga gggctgaaag gccggccaag gcggcgatcc cgatacagaa
       61 gtgaaagcgg ccaaaggccg taggcctggc ggtccggaga gaccaggtcg gcagttaggg
      121 cccgttctct cccttcggcg agtccgaagt ataggccata gaaacctatg agcgcccata
      181 tatgccaagg ctgactggcg gcaccgaaac ccagggaggc gaaggcgtac accagccatc
      241 cggcgatgat tgcgccccgc ctccctttga ggtcggagag catcccgctc ggcagctctc
      301 gcttctcagg ctcgcccacc cgacgcgcga tcagtcagtc ctctccgtgc ttctccagac
      361 tcacgcacgg ttccagcaca tgacaggccc ttgtgccttg gccagatccc tcccccatca
      421 ccacccttgg catcttgcaa cactgcgtct atgcttagct cgatggagaa aaatcgcgat
      481 aacagttctt ccacgcatac gcttagacgc gagacttttt gtgcataggt ctctagcttg
      541 cttattggca atgggtagcg taccatttgt ctacttaatg gatggttcct atcaaaaggc
      601 tatacagccc acctggtgtt cggctagaat gtcccgggat tgcgcatcta aaaaaatctt
      661 ctgaggagaa gttccatgtc aaaaaagcat ccagttattg cagttgccgg ctcatctggg
      721 gcgggtacta cctttgttaa acgtgcgttt gaaaatattt tccgccgcga gggaattacc
      781 tccgcgatta ttgaaggtga tagtttccac agcgtcactc gcacgcaatt caaggaacgc
      841 tctgcagttg aacacaattt cagccacttc ggccccggag ccaacgactt ccacgcactc
      901 gaagcgctgt tcaagagcta tggtgaagcg ggggttggca aaaagcgtca ctacctgcat
      961 aacgctgaag aagcggcatt tcattgcaag cgtatggcgg acgcgggtgt cacctgcaac
     1021 gctggttcag gcgaatttac cccatgggaa gagattgaaa gcaacaccga cgttctgttc
     1081 tatgaaggct tgcacggttt ggtgaaagac gtttccccag atcagaaata cggcggctat
     1141 gacgtagccc agcacgttga cctcggcatc ggggtagtac ctagcgtcaa cttggaatgg
     1201 attcaaaaaa tccaccgtga ccatgctgag cgcggctatt cgcccgaagc aacggtagac
     1261 accatcatgc gccggatgcc ggactacatt aaccatctgg ccccgcaatt ctcacgcacc
     1321 cacgtcaact tccagcgcgt gcctactgtg gatacctcca acccattcat cgcgcgtgat
     1381 atcccttccg cagatgaaag ctttgtggtg atccgctttg ttgatccgaa acgtttcggg
     1441 gttgatttcc cgatcctgtt ggcaatgatc aacggctcat tcatctcacg ccgtaacacc
     1501 atcgtggttc cgggcggcaa gatggtcttg gcaatggaac tgatcttagc cccgatcatt
     1561 cacatcatga tcgagaacaa gctcaaggcg taacgtaacg cccaatgtgc tccgccagcc
     1621 tttgcgccaa tacagggctg gcagttccta acgttgccag aattatggtc actactcagg
     1681 tgtttaggct gcaggctgtt agttgttgcg tatggaccga atcaaggcac ccagaacctt
     1741 ttcggtcccc ttcattttcg ttgagggtcg ataccccgcg gcttgccgcg agttcgtcat
     1801 accggcggaa gccggtatcc agagggcccg actggattcc gtgtcaagca cggaatgacg
     1861 ggccagaaca gaagacgcgc cttgcctgcc gaccagttcg ggtgaaggta ttgcaagctg
     1921 ttggtgcggt ttgccaattt catcttcgca cccctcccgc tgcacaacac tcctacccat
     1981 cccccatctc gctgcccgtt gcggcctcat gtcatcgaaa acgtctcaca aggtgattct
     2041 atcgcactac acccttgatg tctggggaga cggaggacgg aacaggataa agagcaggag
     2101 ggctgaaagg ccggccaggg cggcgccgac ggtgaaggcc ggccaggcgc cgaaggactg
     2161 gtagaggtag cccatcagta ggctgctggg cagggcggcg atcccgatac aggagtgaaa
     2221 cagaccaaag gcagaggctt ggcggtccgg tgggaccagg tcggcgatca aggcccgctc
     2281 tcccccttca gtgagtccga aatacagccc atagaaacca aggagcaccc agatatgcca
     2341 gggttgactg gcggcgccga aacccaggta ggcgacggcg tacaccagcc atccggcgat
     2401 gattgcgccc cgtcttcccc tgaggtcgga gagcatcccg cccggcattc ctgtggccgc
     2461 cttgaccaca tggaaataca tccacagcag cggtaaatgg gcgacggcga tgccgacttc
     2521 ctgcgctcgc aggaggagaa aggcatcgct ggagttgccc agggtaaata gcgtcacaac
     2581 gaagaggaaa cgtgtcagtt gtctattcat gcggccagtg ccagacgttg ctttgggagc
     2641 cgctgtcgga ttgacctgga tgtcgctcac cttcaagata aggatcagga cagagaggat
     2701 gccggggatc gccgcgagca gaaaaatggt cctgagttga ccaccaccca gaagcaggag
     2761 gagcacatag gccagggccg ggcctgctac cgctcccagg tgatccatcg aacgatggaa
     2821 gccataggcc cttcccctga tggccaagtc ggtggagccg gcgataaggg catccctcgg
     2881 ggaggtgcgc agccccttgc caagacggtc cgcaaaccgc accatcagga cgtgccaggg
     2941 gatcagggcg aaggcaacaa gggggcgggt aagcgtggac agggtatagc cgacaaccac
     3001 cagccccttg cgccggccga gccgatcgga cagccacccg gagaatagct tagtcaggct
     3061 ggccgcgctc tcggcgatcc cctccaccag gccgacgaag gtctggcctg ccccgagtac
     3121 ggtggtcaga aacagcggca ggagcggata gatcatctcc gcgctgacgt cggtgagcag
     3181 actgaccagt ccaagcgcca ccacgttccg gctgaggcca aacatgcgaa ccctctgact
     3241 acctgacgcg gtaggtctct atacgctcat gagcggcgcc tcgacccagc caaccggacg
     3301 tcaggctcgt tcgcccttcc aagttcttgt agacgttaaa gaagtgctcg atttctttca
     3361 gtacatgagg ctccacgccg gcaagatcct cgatccgctg atagcgcgga tcgccgtggg
     3421 ctacggcgag gatcttctca tcgatcccct tatcatcgac catgtccagc atcccaacgg
     3481 gccgggcctc gaccaggcaa ccggggaatg tcggctggga gatgaggatc agaacgtcga
     3541 gcgggtcccc atcttgaccg agcgtgcccg ggataaatcc gtagtcggcc ggatagtgca
     3601 ccggtgagta caatacgcgg tccagccgaa acacgcccaa gatcacatcg tattcgtact
     3661 tgttgctgga tcccgaaggg atttccacga cggcgttgac aataaccggg gcactattgc
     3721 caatcggcaa gcttttaaag ttcaccgtcg aatcctcctc atttgactta cactaatgag
     3781 actcattggg gtccgctctg ctcaaggata gagatcagtt gatcgatcag ctcaccgcta
     3841 agcggtagct tgcgcagcgc atccacggtt ccggacagat catacttggc gcgcctcagc
     3901 tcaacaacgc cgtcttcgat gatagcgtag ctacactcag ggttaccatc tcttggctgg
     3961 cccacgctgc cggggttgac gatgacccgc gagccgatac cccgcaccat cggcaggtgc
     4021 gtgtgtccca ggaggatgaa gtcagcctta accccttcta tctgctcgcg gagctgatcg
     4081 tcgggtagtt caggcgtgat ctcatatcca tacagcgggt cacgaggtgt ggcgtggaag
     4141 gctgcaaagc ggtagccgtc gagcgtgaac tcgaccgagc cgggtagcgc ccgcagccag
     4201 tcgaggtcct cgctgctcag gagggtgcga tggtaggcga gggtcgcttc cgccaacgcc
     4261 ttattcgctg gcgagcagcg acactcgacg tcaagcgcca aggcattgtc gtgattgccg
     4321 cggatggcca caaaggcgcg ctctctgatg aacctgatga cctcgtttgg ggagggtcca
     4381 tagccaacca gatcgccaag gcagatcaag cggtcaaact cctccgctgt ggcagcttcg
     4441 agtgccgcca gattggcgtg cagatcggag agtaccagta ttctcattga gtggtcacct
     4501 ctgggttcgc gccattatct cacggtgggg cctgtctcga aagaacttcg tgacgcggcc
     4561 tgaatcaccc gaatcggccg aagattgcct ttgaggtagc catagtgtat gatttagcgg
     4621 atatggcgtg atccgtgtgt gaacaaggag acgcgtgagt gcatagcttt cggcaagtga
     4681 tagtggatga gattctccct gcagtgagta aaccgtcccg gtacatcggg atggaatgga
     4741 atgccgtcaa gaaggacccc gccaacgcga aggtcaaagt cgcacttgcc tttcctgata
     4801 cgtacgaaat cggcatgtct catctgggct tgaagatcct gtaccaggtc ctgaaccggc
     4861 atccagagtt tatggcggag cgggcctatt cgccgtgggt ggatgcggag cggctg
//
LOCUS       DATMXI010000148         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_310561, whole genome shotgun sequence.
ACCESSION   DATMXI010000148 DATMXI010000000
VERSION     DATMXI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310561"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1584)
                     /locus_tag="VN444_02785"
     CDS             complement(232..1584)
                     /locus_tag="VN444_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWR20771.1"
                     /translation="MRQRIQRLRGQGMAMAWLVLCAILAPGLVDAQVAARVKESALEN
                     GLKILLLEEHKAPVVTIHIWYRVGARNEQPGTTGLSHLLEHMMFKGTSKLGPGQFSRV
                     IKRNGGRDNAFTSEDYTGYFETFASDRVELALELEADRMRGLLIDRKEAESERAVVME
                     ERRLRTEDEPISALREVMGATAFQVHPYRQPVIGWMSDIEKLDREDLVRHYNTYYVPN
                     NAVLIVVGDFKSDELLPKIRQHFGAIPRAADPPLVRAIEPEQRGERRLVLKKEAELPF
                     VFMGYHVPNLKHPDNFALEVLAYILSGGKSARIYKSLVYEKQLALFAGGGYDRESIDP
                     NLFPFYASVMPGKTAEEVETALTAEIERVKNEPIPDRELQKAKNQIEAGFLFGQDSVF
                     NLARQLAEYEIVAGWRAWEAYLPGIRAVTAADLQRVARVYLTPDHRTVAVLIPEKMKK
                     "
     gene            complement(1603..2073)
                     /locus_tag="VN444_02790"
     CDS             complement(1603..2073)
                     /locus_tag="VN444_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HWR20772.1"
                     /translation="MRAIQNLFIQGLPFFFPLLLYVLLASDVSALQGGGVVIDGRVTI
                     AVEVARTAQEQARGLGGRSSLPKGGGMLFPFDVAERRIFWMKGMLIPLDILWIREGKI
                     MAIDANVAPPRSHETPAIVSHVADLVLEVPAGFALEMGISVGQTVRVRYEGSSR"
     gene            complement(2070..3188)
                     /locus_tag="VN444_02795"
     CDS             complement(2070..3188)
                     /locus_tag="VN444_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWR20773.1"
                     /translation="MPAHFSVQVRRAVLTALLTVGCLAVGTHALAQKTQVESPGLSPE
                     EQIIISVYKHASPGVVHITSTALAYDVFFNAVPQKGTGSGFVVDDRGYILTNNHVVEE
                     ADSLEVTLTDKSKVPAKLIGRDPSNDLAVIKISVPKEKLFPLKLGSSDALQVGQMAIA
                     IGNPFGLDRTVTRGVVSSVGRTLRSGSGRQIRGIIQTDAPINPGNSGGPLLNSHGEVI
                     GMNSAIYTPSGGSVGIGFAIPIDTAKRLLPQLIAKGRVSHPWLGVAGLDITPEVASAL
                     KLPVRQGIVVMQVSPKGPVDRAGIRGSTRKARVGNMLVSVGGDIIVAVESRKVTSIDD
                     LTAFLDGERKVGDQVKIELLRDGRPLALSVRLGELPEK"
     gene            complement(3370..3516)
                     /locus_tag="VN444_02800"
     CDS             complement(3370..3516)
                     /locus_tag="VN444_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWR20774.1"
                     /translation="MAKDPVCKMMVDENRAAATAVYQGVTYYFCAVGCKRAFEQSPEK
                     YIST"
     gene            complement(3528..3947)
                     /locus_tag="VN444_02805"
     CDS             complement(3528..3947)
                     /locus_tag="VN444_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3630..3632),aa:Sec)
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HWR20775.1"
                     /translation="MKRDEVEQPDRRLALRILVGGLFGLFVAPVAGWAAGGDSAALAT
                     PRRPRPATPSPALFTGKLAETYRIAKEAPELLEQMPCYCGCGNMANHQNNLDCYADRH
                     ADTUVMCQDITLDAYAMYKQGKKIAEIRAAIDRKYAR"
BASE COUNT          825 a         1321 c         1036 g          850 t
ORIGIN      
        1 gagtagcagg agacctaatg ttgtgaccaa tcggtatcgt cgcattacgc tatgtatcgc
       61 ctttcgcatc ctgcaccaaa ccccctcccc ccttatcaaa ggggaggagt gagacaacgg
      121 gcgcagcaag cggcgcccct acaccatgca ccaaatcttt ccgttccccc ctttgataaa
      181 ggggggtgag ggggggttac tccacactga gggcagccgc aagggctgcc cctacttctt
      241 cattttctcc gggatcagca ccgcgacggt gcggtgatct ggggtcaggt agactctcgc
      301 gacccgttgc agatccgccg ccgtgacggc gcggataccg gggaggtacg cctcccatgc
      361 gcgccagccg gccacgatct catactcggc cagttgcctg gccagattga acaccgaatc
      421 ctgcccgaag aggaagccgg cttcgatctg attcttcgcc ttctgcagct cccggtccgg
      481 gataggctcg ttcttcacac gctcgatctc tgccgtcaga gccgtctcaa cttcctcagc
      541 cgttttgccc ggcatcacgc tggcgtaaaa cgggaagagg ttcgggtcga tgctttcccg
      601 gtcatacccc ccgccagcaa acagggcaag ctgtttctcg tagaccaggc tcttgtaaat
      661 cctggcgctc ttgccgccgg acaggatata ggccagaacc tcgagggcaa agttatcggg
      721 gtgtttcaga ttcgggacat ggtaccccat aaacacaaac ggcagttccg cttccttctt
      781 caggacaagc ctccgctctc cccgttgctc cggttcgata gcgcggacta agggcgggtc
      841 agcagcccgc ggaatcgcgc cgaagtgctg tcggattttt ggaagcagct catcgctctt
      901 gaaatcgccg accacgatca gtacggcgtt attcggaaca tagtaggtgt tataatggcg
      961 taccagatcc tcccggtcca gcttctcgat gtcagacatc cagccaatga caggctgccg
     1021 gtacgggtgc acctgaaacg cagtcgcccc catcacctcc ctcagggcag agatgggctc
     1081 atcttctgtc cgcagccggc gctcctccat aactactgcc ctctccgatt ccgcctcctt
     1141 ccgatcaatg aggagaccac gcatccgatc ggcctcgagc tccagggcca gttcgacccg
     1201 atccgaggca aacgtctcga agtagccggt ataatcctcg ctggtgaagg cgttgtcgcg
     1261 accgccgttt ctcttgatga ccctcgaaaa ctggcctggc cccagcttcg aggtcccctt
     1321 gaacatcatg tgctccagca ggtgagacag gccggtggtt ccgggttgct cgttgcgcgc
     1381 gccaacccga taccagatgt ggatcgttac cacgggcgcc ttatgctctt caagcagcag
     1441 gatcttcagt ccgttctcga gcgccgactc cttcaccctg gccgcgactt gcgcgtccac
     1501 gaggcctgga gccaggatag cacagagaac caaccaggcc atcgccattc cctgtcctcg
     1561 caggcgctgt atccgctgtc tcatcgccgc ttccatggcg cgctaacgac tcgatccttc
     1621 atatctgaca cggaccgtct gacctacgct gatgcccatc tccagggcga agcctgccgg
     1681 gacctccagc accagatcag ccacgtgact gacgatggcg ggggtctcat gtgaacgcgg
     1741 cggagcgacg ttcgcatcaa ttgccatgat cttaccctcc cggatccaaa gaatatcgag
     1801 cgggatcagc atccccttca tccagaatat ccggcgctct gccacgtcaa agggaaagag
     1861 catccctcca ccctttggaa gcgatgatcg accccctagc cccctcgcct gctcctgtgc
     1921 ggttcgcgct acctcaacgg caatcgtgac ccggccgtca atgaccaccc cgcccccttg
     1981 aagggccgag acatcagagg ccaggagtac gtatagcaga agagggaaaa agaaagggag
     2041 cccctggata aagagattct gaatcgccct catttctcag gcagctctcc cagtcgaacc
     2101 gagagggcga ggggccggcc atccctgaga agctcgatct tcacctggtc tcccaccttt
     2161 cgttccccat ccagaaaggc cgtcaggtcg tctatcgatg tcacctttcg gctctcgacc
     2221 gcaacgatga tgtcccctcc cacactgacc agcatgttac cgacccgcgc ctttcttgta
     2281 cttcccctga tacccgcacg gtccaccgga ccttttggag agacctgcat caccacgatc
     2341 ccctgtcgaa ccggaagctt cagggcgctg gccacctctg gcgtgatgtc caggccggcg
     2401 acgcccagcc atggatgact gacccgcccc ttggcgatca actgggggag cagtcgcttc
     2461 gccgtatcga tggggatggc aaaaccgatt ccgacagacc cgccactcgg cgtgtagatg
     2521 gcgctgttca ttccaatcac ctcgccatgc gagttgagca ggggaccacc tgaattgcca
     2581 ggattgatcg gcgcgtcagt ctggatgatg cctcgaatct ggcgaccgct cccggagcgg
     2641 agtgtccgac ccaccgagct gaccacccca cgcgtgaccg ttcgatccag accgaacgga
     2701 ttgccgatgg cgatggccat ctgcccaacc tgcaaggcat cgctacttcc cagcttcaaa
     2761 gggaacagct tctccttcgg aacagaaatc tttatcactg ccagatcgtt gctagggtca
     2821 cggccgatca actttgccgg caccttactc ttatcggtta acgtcacctc cagactgtca
     2881 gcctcttcca ccacatggtt attagtcagg atataccccc ggtcatccac cacgaagccc
     2941 gatccggtcc ctttctgggg caccgcatta aagaacacat cgtacgctaa agcggtgctg
     3001 gtaatgtgaa caacgcccgg gctggcatgt ttatagactg agatgatgat ctgttcttca
     3061 gggctgagac caggactttc cacctgggtt ttttgtgcta aggcgtgggt cccaactgcg
     3121 aggcatccaa cagtcaagag cgcagtcaac actgcgcgcc ggacctgaac ggaaaagtgg
     3181 gcaggcaaat gtctcgctcc ttcgattgcg tcagtagcca aaaaagaaac gtggaccctc
     3241 cgtccacctc gatgactgta gaacaccacc gtgggcttgt caacgcccaa ggcggctaat
     3301 ccagctttgc gagcaattag agtctacagg gcgaggccca tagcacttcc cctctctggc
     3361 ctcgtactcc tacgttgaaa tgtatttctc cggcgactgc tcaaaggccc gcttgcagcc
     3421 tacagcgcag aagtagtagg tcaccccttg atagacggct gtggccgccg cccgattttc
     3481 atccaccatc atcttgcaca cgggatcttt ggccatcgtt cctcctctta cctggcatat
     3541 tttcggtcga ttgccgctct gatctctgcg atcttctttc cctgcttgta catggcatac
     3601 gcatccagcg taatgtcttg gcacatcact cacgtgtccg catgccgatc cgcgtagcaa
     3661 tccaggttgt tctgatgatt ggccatgttg ccacaaccac aatagcacgg catctgctcg
     3721 agcaactccg gcgcctcctt ggcgatccga taggtttctg ccagcttccc agtgaacaat
     3781 gccggagagg gtgtagccgg ccgcggccgt ctcggggtcg ccaaagccgc actatcgcct
     3841 cctgctgccc aaccggcaac aggcgcaaca aataaaccga acagcccccc gacaaggatc
     3901 ctgagagcca atcgtcggtc aggctgttcc acttcatccc gcttcatacg tcttcacccc
     3961 cttccttgtg catggaccca aaacagtatc ctgctcaaca atagttgtct attgcctgta
     4021 tggtccccca tc
//
LOCUS       DATMXI010000149         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_142609, whole genome shotgun sequence.
ACCESSION   DATMXI010000149 DATMXI010000000
VERSION     DATMXI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142609"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN444_02810"
     CDS             complement(<1..300)
                     /locus_tag="VN444_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20776.1"
                     /translation="MKITRLKDHALLKTASAVSREYIAKYNPDDPKNSPSASRKESYA
                     IETLLSVGNVVSCIDQLHFSMDLLSGYRSSSTPDKMSRYDYIVFGIENYYLRLTSV"
     gene            complement(388..1212)
                     /locus_tag="VN444_02815"
     CDS             complement(388..1212)
                     /locus_tag="VN444_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HWR20777.1"
                     /translation="MARLSKKAAERIAVGLKRFQPILASAKSRDVNESDTVVIVADLL
                     QDIFGYDKYAEITSEHMIRGTFCDLAVKLEGTWAFLIEVKAIGLELKEQHIKQAVDYA
                     ANQGCEWVALTNATVWKVYKVTFSKPIQHELVVDLDLLALNHRNSEHIELVGLLAKEG
                     WQKAQLGEYHSQKQALSRFTLAALVLSDPVLEVLRREIRRLSPDVRVETEEIKSALET
                     DVLKREVLEGEKADVARKHAAKASGRALRASKSGSPSPAESTSTGEAATFPSEVPK"
     gene            complement(1726..>2142)
                     /locus_tag="VN444_02820"
     CDS             complement(1726..>2142)
                     /locus_tag="VN444_02820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWR20778.1"
                     /translation="AQVGEGLVLGADTIVECDGRPLGKPKDLDEARWFLCLLSGRSHL
                     VVTGLALVQTGDARTEVGHEVTEVQMRALTGAEIDTYVRTDEPLDKAGGYAIQGAAES
                     FIEGIKGSFTNVVGLPLGKLRTLLLRFGIDPLHRQV"
BASE COUNT          502 a          605 c          557 g          478 t
ORIGIN      
        1 aactgacgtt aagcgcaagt aataattttc aatgccaaat acgatgtagt cgtatcggct
       61 catcttgtcg ggtgtgctac tcgacctata gccagacagc aagtccatag aaaaatgaag
      121 ctgatctata caactaacaa cgttgccaac agaaagaaga gtctcgatgg cgtaactttc
      181 cttcctggac gccgaaggac tattcttggg atcgtctggg ttgtacttgg caatatactc
      241 tctactgacg gccgatgctg tcttaagaag cgcatgatct ttgagacgtg taatcttcat
      301 ctatctaccg tataacgaaa acactgagtc gccgaccaca aggcaactgt aaaatcgcct
      361 ccagtaaatt gttggacagc gcaaaggtta ctttggtacc tctgacggaa aagtggcggc
      421 ttcaccggtc gatgtcgact ctgcgggaga tggagacccg gacttggaag cgcgaagtgc
      481 cctgccggat gctttggccg catgcttccg cgcaacatct gccttttctc cttcgagaac
      541 ctctcgtttt aggacgtcgg tttccagagc gctcttgatc tcctccgtct ccactcgaac
      601 atctggcgat agacggcgga tttctcggcg tagaacctca agcactggat cgctcagaac
      661 gagggcggcc agagtgaacc ggctcagggc ctgtttttgc gagtgatatt cgccaagttg
      721 ggccttttgc caaccctcct tggcaagaag gccgacgagt tcgatgtgct ccgagttccg
      781 gtgattaagc gccagcaagt ctaggtcgac aacgagttca tgttggatgg gcttcgaaaa
      841 agtgacttta tacaccttcc agactgttgc gttcgtgagc gctacccact cgcacccttg
      901 gttcgcagca tagtcaacag cttgcttgat gtgttgttcc ttgagctcga ggccgatggc
      961 cttcacctcg attaggaaag cccacgttcc ttcaagcttg accgccaaat cgcaaaaggt
     1021 cccacgaatc atgtgttcgg aggtaatctc ggcgtacttg tcgtaaccga agatgtcctg
     1081 caggagatct gctacaatga caacggtatc ggactcgttc acatcgcggg acttcgcaga
     1141 agcgagtatc ggctgaaacc gcttcaatcc tacggcgatc cgctctgctg ccttctttga
     1201 aagtcttgcc atgagtctcc tccctgctat acggttcgag atgcgacatc caacagatat
     1261 tagccgacac ttcttggagg gactctacga tagtgatgca taaaaagcaa cacgatactc
     1321 acggcgcaac aacagagaga tagtggcgtg tcattaagcg tggaaggtaa cagtgtggga
     1381 gactttctgg gcaatatcgc atgcgcctgc ggtacaccca tgtgcatcaa agtctagacc
     1441 acgcaccctc agtaaaaccc cccacacccc ccttttgcaa agggggggac aggaagattt
     1501 caggggaagg aatcttccca gactttcctg ccaagagcgg tcaaacggga tgcggtcgaa
     1561 gcgggcgggt aggtcattcg cacggtccag gaacttctcg gccacgcaga cgttagcacg
     1621 acgatggtct acacacacat gcggggcaga gggccatgtg taacgttcag cgatgagcca
     1681 gagcaccgct gccaactcct gatggcggct cgctaaagac tgccctcaga cctgccgatg
     1741 gagcggatcg atgccgaatc gcaggaggag cgtgcgcagc ttgccgagcg ggagtccgac
     1801 cacgttggta aagctgcctt tgatcccttc aatgaaactc tcagcagctc cctggatcgc
     1861 gtagccccca gccttatcaa gcggctcatc cgtccgcaca taggtgtcga tctccgcacc
     1921 cgtcaaggct cgcatctgta cctcggttac ctcgtgtccg acctctgttc tggcatcgcc
     1981 agtctgcacg agggcgagcc cggtcaccac cagatgactc cgaccactca acaggcatag
     2041 aaaccagcgg gcctcatcca gatctttcgg cttacccaga gggcgaccgt cacattcgac
     2101 gatggtgtcg gcgccaagga ccaacccctc ccctacctga gc
//
LOCUS       DATMXI010000150         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_311300, whole genome shotgun sequence.
ACCESSION   DATMXI010000150 DATMXI010000000
VERSION     DATMXI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311300"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN444_02825"
     CDS             <1..445
                     /locus_tag="VN444_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWR20779.1"
                     /translation="LQIDMPERVFAEPLNEEVARFVGIETILRGHVQSVSQGLATVEI
                     PGWALEVAAPLTPGERVLVCLRPEEITLFPRGATLTTSSARNQLCGRVVRHLPAGSTY
                     RVTIDCGALIVATVTRQSWEQLDLHDGAEVVAAFKATAPHVIHCA"
     gene            655..903
                     /locus_tag="VN444_02830"
     CDS             655..903
                     /locus_tag="VN444_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20780.1"
                     /translation="MEETETQLFARLWEENSEFQRLAEKHREFDLKISEFDRIYYLTS
                     EQERKRKELQKLKLTIKDQMHVIMHQYRSNHTPTTSKK"
     gene            900..1397
                     /gene="moaC"
                     /locus_tag="VN444_02835"
     CDS             900..1397
                     /gene="moaC"
                     /locus_tag="VN444_02835"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWR20781.1"
                     /translation="MKRLTHVDRHGQARMVDISEKDETRREAVARGTVTMQPVTLRMI
                     QKGMVPKGDVLAAARIAGVMAAKRVPDLIPLCHSLLLSSAEVEFTPVEKAGRLDIESR
                     VKVTGRTGAEMEALAAVAMAALTVYDMCKAVDKEMVIGSIRLVAKTGGKSGVYRRLGE
                     NGAGG"
     gene            1448..1885
                     /locus_tag="VN444_02840"
     CDS             1448..1885
                     /locus_tag="VN444_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20782.1"
                     /translation="MPSGLTTAFMYTPRFLEFDYGPGHPLRNERLGLTYGLISARGVL
                     SLPSIRYVEPEPATDDELLVFLKPDYLKVLKAADAGDAPAGAFQYDLGTPDNPILPGI
                     FRWSALDVSCFGCEVAGSGSSKLEIRNQELSDKAYPWRRFEGM"
     gene            2062..2352
                     /locus_tag="VN444_02845"
     CDS             2062..2352
                     /locus_tag="VN444_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HWR20783.1"
                     /translation="MAFQDRSLTCVDCGQQFVFTAGEQEFYEQKGFMNEPKRCKGCKA
                     VRKGTGGHGGPRQEFEVVCSACGQQTSVPFKPTLDKPVFCKPCFVAKRSTMA"
     gene            2746..>3822
                     /gene="selA"
                     /locus_tag="VN444_02850"
     CDS             2746..>3822
                     /gene="selA"
                     /locus_tag="VN444_02850"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HWR20784.1"
                     /translation="MDSRTRLLLRQLPSVDDLLQEPSIREMARTLPRWAVVEAIREVL
                     ERWRRMLATGQSESAPADLPSRAALVVEVKRIALRLNRPALRRLINATGVAIHTNLGR
                     APLAEAGIERIVEVARGYSNLEYDLERGDRGSRQDHVERLLCRLTGAEAALAVNNNAA
                     AVLLAINTLAEGKEVIVSRGELVEIGDSFRIPDIMRRAGGVLREVGTTNRTYLRDYEE
                     AVGATSAIILKVHTSNFRIQGFASQVPVAELASLGEKTGLPVVEDVGSGALVDLSQIG
                     LSKEPMPSESIRAGADLVTFSGDKLLGGPQAGLIVGKRELVEKLRRNPLARAVRIDKL
                     TLAALEATLRLYLDERRAFAHIPVL"
BASE COUNT          813 a         1013 c         1235 g          761 t
ORIGIN      
        1 gctgcagata gacatgccgg agcgagtctt tgccgagcct ctcaacgagg aggtggcccg
       61 tttcgtcggc attgaaacga tcctgagggg ccacgtgcag tccgtaagcc aggggctcgc
      121 cactgtcgag attccggggt gggccctgga agtggcagcg ccattgacac cgggggagcg
      181 cgtgctggtc tgcctcaggc cggaggagat cacgctcttc cctcgcggag caaccttgac
      241 cacctccagc gcgcggaatc agctctgcgg gcgggtggtc cgccatctgc cggccggctc
      301 cacctatcgg gtgacgatcg actgcggcgc cctgatcgtg gcaacagtca cccggcagtc
      361 gtgggaacaa ctcgacctgc acgacggggc cgaagttgtt gccgcattca aagccaccgc
      421 tccccatgtt atccactgcg cctgacggta ttgtccttga cttccgtccc atagtttggt
      481 aagttaaaaa acaaaatggg gtggccctca caacatatgc ctgcctttac gtataagcag
      541 tccagcctgc cagcgctgag aggcgatcgt cggcgtgagc cttttggcgt cgagttccat
      601 ccctcttcga gatgtcgcca ttgcggtcaa aatccacccg ggaggaggta aaagatggaa
      661 gagacggaaa cacaactctt tgctcgtctc tgggaggaga attcagagtt tcagcggcta
      721 gccgagaagc atcgagaatt cgatctgaag atcagcgagt tcgaccggat ctactacctg
      781 acgagcgagc aggagcgaaa gcggaaggag ctacaaaagc tgaagctgac aatcaaggac
      841 cagatgcacg taattatgca tcaatatcgg agcaaccaca cgccgactac gtccaaaaaa
      901 tgaaacggct gactcacgta gaccgacatg gccaggcccg catggtcgat atcagcgaga
      961 aggatgaaac caggcgggag gccgtagcta gaggcacagt taccatgcag cccgtgaccc
     1021 ttcgcatgat ccagaaaggg atggtcccga agggcgatgt cctggccgct gccaggatcg
     1081 ccggggtcat ggcggcaaaa agggtgcctg acctcattcc gctctgtcat tctctcctgc
     1141 tctccagcgc cgaggtcgag tttacgccgg tagagaaagc cggccgactc gatatcgaat
     1201 cacgggtcaa ggttacggga cgaaccgggg cagagatgga ggccctcgct gctgtggcta
     1261 tggcagccct gaccgtctat gacatgtgta aggcggtaga caaggagatg gtgatcggat
     1321 caatccgtct ggtcgctaaa acagggggaa agagtggggt gtatcgtcgg cttggcgaga
     1381 acggagccgg gggctgaaag gcccctgtaa gggattaagg gccccggtat acagctcggc
     1441 gattaggatg ccttcaggac tgacgacagc gttcatgtac accccgcggt tcctggaatt
     1501 cgactatgga ccggggcatc cccttcgcaa tgagcggctg gggctgacct acggtttgat
     1561 cagcgcccgt ggggtactgt cccttccttc tatccgctat gtcgaacctg agcccgccac
     1621 tgacgacgaa ctcctggtct ttctgaagcc cgactatttg aaggttctca aggcggccga
     1681 tgctggtgac gctcctgccg gagcattcca gtacgacctc ggcactccag acaatccaat
     1741 tctgccggga atattccggt ggtctgcgct ggatgtttcg tgtttcgggt gtgaagttgc
     1801 gggttcaggg tcctcgaaac ttgagatacg gaaccaggaa ctttccgaca aggcgtatcc
     1861 ttggaggagg ttcgagggga tgtagggctt ctcaagaggt tggtgtttcc gcggcacggc
     1921 atctgatgtc ggaccgcttt tatcggtggg gagagcctat gatgaggcta tgagacgggc
     1981 tagacgggat tgatgacagg acagggcgtc gaggtgctgg agcagggcaa aaacgggttt
     2041 cggtttggca ggaggcagcc aatggcattc caggataggt cactcacgtg tgttgattgt
     2101 gggcagcagt ttgtctttac cgcgggagag caggagtttt acgagcagaa aggattcatg
     2161 aatgagccga aacgttgcaa gggctgcaag gcggtgcgca aagggacagg cggccacggc
     2221 ggtccccgcc aagagttcga ggtagtctgc tcggcctgtg gccagcagac ctctgttcct
     2281 ttcaagccga cactcgacaa accggtcttc tgtaagccct gtttcgtggc caagcgatcg
     2341 acaatggcgt gagatggtaa cgacttaggc agatagatgt aggctgttag actataggaa
     2401 cgcgcacgga tgagccaggc gtttccgact gcgacgcgac aatgatggag accgaaaagg
     2461 ttctgggccc cttgattcgg tccatgcgca acgactaaca gccttcagcc ttcagcctaa
     2521 acacctgagt agttacgtga gatgtgcaaa tctgaatagc ctgtcgttgt aagtgtgtat
     2581 gaagggccgg gcgagtctat gacagcccgg cccttcgcct tccggatggg atcacagagg
     2641 cgatctctga gttgaatcga gaggggaagt atgatagcgt gacgataagg cagctatcag
     2701 ctaacagctt tcagcgatca gctattgact gaaggaaaag gttctgtgga tagtcgaacc
     2761 aggctcctgc ttagacagct tccctcggtg gatgatctgt tacaggagcc gtcgatccga
     2821 gagatggctc ggacgctgcc gcgctgggcg gtcgttgagg cgatccgaga ggtgttggag
     2881 cgttggcggc ggatgttggc taccgggcag tccgaatcgg cgccggccga tctgccatcc
     2941 agggctgctc tggttgtcga ggtaaagcgg atcgcgctgc ggctgaatcg gccggccctg
     3001 cggcgtctca ttaacgcaac cggcgtggcc atccatacaa acctcggtcg cgccccactc
     3061 gccgaggccg gaatcgagcg gattgtcgaa gtagcgcggg gctactcgaa tctggagtat
     3121 gaccttgaac ggggggaccg ggggtccagg caggaccatg tggaacggct cttatgccgt
     3181 ctcactgggg ccgaggccgc gcttgcagta aacaataatg cggcggcggt gctgctcgcg
     3241 attaatacgc tcgccgaggg gaaggaagtc atcgtctcgc gcggagagct ggtagagatc
     3301 ggcgactcgt ttcggatccc cgatattatg cgtcgcgcag gcggggtctt gcgggaagta
     3361 gggactacca accggaccta cctgagggat tatgaggaag ctgtcggggc gacgagtgcg
     3421 atcatcctga aggttcacac cagcaacttc cgaatccagg gctttgccag tcaggttccg
     3481 gtggccgagt tggcgagcct gggcgagaag accggccttc cagtggtgga ggatgtgggc
     3541 agcggcgccc tcgtcgatct gtcacaaatc gggctctcca aggagccgat gccatcggag
     3601 agcatccgtg ccggagctga cctggtgacc ttcagcgggg acaagctgct gggaggacct
     3661 caggcaggcc tgatcgtagg gaagcgagag ttggtcgaga agctccgtcg caatccgctc
     3721 gcgcgtgccg tgcggatcga caagctcacg ttagctgctc tggaggcgac gctgcgcctc
     3781 tacctcgatg agaggcgagc ctttgcccac attccggtac tc
//
LOCUS       DATMXI010000151         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_198819, whole genome shotgun sequence.
ACCESSION   DATMXI010000151 DATMXI010000000
VERSION     DATMXI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198819"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="nth"
                     /locus_tag="VN444_02855"
     CDS             complement(<1..630)
                     /gene="nth"
                     /locus_tag="VN444_02855"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWR20785.1"
                     /translation="MITNRQIDKVLQSVRRAISVWEPAVVGKIAEDSRDPFRVLISCI
                     LSQQTKDPVTGEASERLYRLADQPDTMLALSELHIAQAIYPVSFYKTKARTIRKVCQA
                     LLTQFAGRVPDTIEALLSLNGVGRKTANLVVTVGYRKSGICVDTHVHRISNRWGYVGT
                     NTPDHTEMALRLKLPKRHWIHYNDLLVPFGQNLCRPISPFCSRCPIERWC"
     gene            complement(688..1344)
                     /locus_tag="VN444_02860"
     CDS             complement(688..1344)
                     /locus_tag="VN444_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWR20786.1"
                     /translation="MKFPFTLDRLAAQDIRMIFRVVAEFVDGLEALAAIPPAVSVFGS
                     THIGPDDPAYTMAEEIGRLLAQHGYAVLTGGGPGAMEAANKGAYEAGGVSIGLNIELP
                     QQQEPNRYLTSLVNFQHFFVRKVMFVKHSIAFVILPGGYGTLDEMFESITLIQTRKIK
                     PFPVILVGNDYWHGLHEWLRDPVMNEGKITPNDFATLKIAHNPEEVIQLIKDSSTSGL
                     "
     gene            1502..1846
                     /locus_tag="VN444_02865"
     CDS             1502..1846
                     /locus_tag="VN444_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20787.1"
                     /translation="MLDIRYRIDRMKVLQALRESGLTETQAQRLDELCQARDEDGMLA
                     LLEGATLTPLARKTFEILRQARLVGERLTELSRTIPLPHEKIQELYPQMRNIKLEYER
                     LTTEADRAMTRV"
     gene            1888..2103
                     /locus_tag="VN444_02870"
     CDS             1888..2103
                     /locus_tag="VN444_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263597940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1924..1926,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HWR20788.1"
                     /translation="METLIVIYGKDTUPYTSAAREDYAKRNVPFTYLNVKNDPKALAQ
                     MLEVSKGSREVPVIIEKGKVTIGFGGT"
BASE COUNT          553 a          637 c          740 g          489 t
ORIGIN      
        1 gcaccagcgc tcgatagggc agcggctgca aaagggagaa atcggtcggc agaggttctg
       61 accgaatggg accagaaggt cgttgtaatg gatccagtgc cgcttcggca gtttcagccg
      121 aagagccatc tcggtgtgat ctggagtgtt ggtaccgacg taaccccagc gatttgagat
      181 ccgatggaca tgcgtatcga cgcagatccc cgactttctg tagccgaccg tcacgacaag
      241 gttggcggtc ttccggccaa ccccattgag gctgaggagg gcctcgatag tatcggggac
      301 gcgtccggca aattgggtga ggagagcctg gcagaccttg cggatggttc gagccttcgt
      361 cttatagaaa ctgaccgggt agatggcttg ggcgatgtgc agctcactga gtgcgagcat
      421 cgtgtcaggc tgatcggcaa gccgatacag ccgctccgag gcttcacccg tgacgggatc
      481 cttggtctgc tggctcagga tgcaagagat caagacccga aacggatcgc gggagtcctc
      541 ggcgatctta cctacgacgg cgggctccca gacggagatt gctcggcgaa cagactgtaa
      601 caccttatca atctgcctgt tggtgatcat actcctatcc tggaggatgc cgacctggac
      661 gctgatgaaa agggccggct agggggttta gagtccggag gtcgaagagt ccttaatgag
      721 ttggatgacc tcttcagggt tatgggcaat cttgagcgtt gcgaagtcgt tcggcgtgat
      781 cttcccttcg ttcatgacgg gatcgcggag ccactcgtgt aagccgtgcc agtagtcatt
      841 gcctaccagg atgactggga acggcttgat cttcctagtc tggatcagcg tgatcgactc
      901 gaacatctcg tccagcgtgc catagccacc cggtaggatg acgaaagcga tggagtgttt
      961 cacgaacatg accttccgga cgaagaagtg ctggaaattc acaaggctgg tcaggtatcg
     1021 gttgggttcc tgctgttgag ggagctcgat gttgaggccg atcgaaacgc cgcccgcctc
     1081 gtaggcgcct ttgtttgccg cctccatggc tccaggaccc ccgccggtga gaacggcata
     1141 cccgtgctga gccaataagc ggccgatctc ctcggccatg gtgtaggcgg gatcgtcggg
     1201 tccgatatgg gtcgatccga agacagagac ggccggcgga atcgcggcga gcgcctccag
     1261 cccgtcgaca aactctgcca cgacgcggaa gatcattcgg atgtcttgcg cggccaggcg
     1321 atcaagggta aatgggaatt tcatgctggg tccgtttcct cctctaccat gccttcataa
     1381 attgtaacac atcaaatcat ggccagatag tggcccctgc gttcaagttt tggcaccgag
     1441 gcgtaaggtg tggtatgatt aaacccgata aatcatcaga gtgcgagcct aaggagagac
     1501 gatgctcgat atccgctatc gaatcgaccg gatgaaggtg ctgcaggccc tgagggaaag
     1561 cggtctcacc gagacccagg cgcagcggtt ggacgagcta tgccaggcac gagatgagga
     1621 cgggatgctt gccctcctgg agggggcgac gttgaccccg ctagccagaa agacgttcga
     1681 gatcctcaga caggctcggc tggttgggga gcggctgact gagctgagcc ggaccatccc
     1741 gctcccccac gagaagattc aggagctata cccgcagatg cgcaatatca agcttgaata
     1801 tgagcggtta accacagaag ctgacagggc catgacacgg gtctaacgcg cagaaatcag
     1861 acggaatgta gagagtggag gaggcagatg gagacattaa tcgtcattta cgggaaggat
     1921 acctgaccct acacctcggc ggcccgtgag gactacgcca agcggaacgt cccgttcacc
     1981 tacctcaatg tcaagaacga cccgaaggcg ctggcgcaga tgttggaggt ttcgaagggt
     2041 tcgcgcgagg ttcccgtcat tatcgagaag ggcaaagtaa ccatcggatt tggcgggacc
     2101 tgagcagtct agaggcgcca acggtcgttg gcttaggtga ggaagagaga tgaaatttga
     2161 aacgcggcgc agcgtcagta taatttcagg attagtcctg cttgcaacct ccatgttcat
     2221 gatgaagtac cttgtaaagc tgttaatctg gttgggataa actctaactc cgggttccga
     2281 cacctcccgc agtagactga tacccgcagg actcatccat ccgtttttcc cgaacctccc
     2341 ccattccgac gaggcgctcg taaaaaccaa ggcgaaccca gcttacgcac gaatacccac
     2401 aagccgcgtg gggggcaag
//
LOCUS       DATMXI010000152         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_233144, whole genome shotgun sequence.
ACCESSION   DATMXI010000152 DATMXI010000000
VERSION     DATMXI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233144"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /gene="leuS"
                     /locus_tag="VN444_02875"
     CDS             complement(<1..2212)
                     /gene="leuS"
                     /locus_tag="VN444_02875"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWR20789.1"
                     /translation="MSKTYNHQALEEKWQAYWEQHRTFRVAEDQTKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMKGFNVLHPMGWDAFGLPAEQYAVKTGVHPAITT
                     AQNIETFKRQMKRIGLSYDWEREISTIDPDYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALGTVLANEEIIDGKSEVGGFDVVRKPMRQWVLKITAYADRLLEDLKLVEWPASTLE
                     MQKNWIGRSIGAEVEFDLAGATGTVRVYTTRPDTLFGATYMVLAPEHPLVKVVTTAEY
                     LTLVTAYCEAASRKSDLQRQELEKEKTGVFTGGYAVNPVNNEPLPIWIADYVLMGYGT
                     GAIMAVPAHDERDWAFAKTYDLPIREVIAGGQVEQAAFVATDKGTVVNSTTPDGSFTI
                     DGLAPSDAIPKITSWLESRGKGRKAVNYKLRDWLFARQRYWGEPFPIVWVDGQALPLP
                     EEQLPVLLPETSNFKPSGSGESPLANLTDWLTTTDPVSGVPARRETNTMPQWAGSCWY
                     YLRFIDPRNQGSLIDLTKERYWMPVDLYIGGSEHAVLHLLYARFWHKILFDIGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEIMDHFGADAVRLYEMFMGPLEAVKPW
                     STRGVEGVTRFLERAWRLMVNEEGHVSSSVVETVPTLEQQRLLHQTIKKVTEDIEALR
                     FNTAISQMMVFTNEMTKAEQRSRGLLEPFVLILAPFAPHLAEELWEVLGHQPSVS"
     gene            complement(2381..>3144)
                     /locus_tag="VN444_02880"
     CDS             complement(2381..>3144)
                     /locus_tag="VN444_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWR20790.1"
                     /translation="TGVSTKTTALVFVPGIRYTLLVTPYPLPLTPHVVDWSQIDDVLL
                     DMDGTLLDRHFDNFFFEEELPRRYAALHDLQLEESRDTLLEMYRSVEGELAWTDLHYW
                     TRRVGIDVVALHRELDHMIGFLSGAEEFLQHLHQLGKRVTIVTNAHESGVAIKVAKTG
                     LDRHVDRIVNAFEVGYLKMRPEYWPSCQRLLGFDVARSFFMDDDEHCLTAAKEFGVAH
                     LIHSAKSSSQLPPTPLPQFVSVTDFSPLFSRRPVI"
BASE COUNT          693 a          886 c          837 g          728 t
ORIGIN      
        1 gagaaacgct tggttggtga ccgagcactt cccataattc ttccgccagg tggggggcga
       61 aaggcgccag gatcaagacg aatggttcga gtaagccacg ggaacgctgc tcggccttcg
      121 tcatctcatt ggtaaatacc atcatctggg agatggcggt attgaaccgc aacgcttcta
      181 tatcctccgt cacctttttg atcgtctgat ggagcaaccg ttgctgttcg agggtaggaa
      241 ctgtctccac cacactactt gagacatgcc cctcttcatt caccatgagc cgccaggccc
      301 gttcgagaaa ccgtgtcact ccttctacgc ccctcgtact ccagggcttc acggcttcca
      361 gcgggcccat gaacatttca tagagccgca ccgcgtcggc tccaaagtga tccattatct
      421 cgtctggatt gactacgttg ccgcgtgatt tcgacatctt ctggttgtct tctcccagca
      481 cgatcccctg atgcaccaat ttcttgaacg gctccggtgt actgaccacc ccgatgtcga
      541 acaaaatctt gtgccaaaac cgcgcataga gcaaatgcaa caccgcatgt tcgctcccgc
      601 cgatgtagag atccaccggc atccaatagc gttctttggt cagatcgatc aacgaacctt
      661 gatttctcgg atcgataaat cggaggtagt accagcagga gccggcccac tgaggcatgg
      721 tgttggtttc gcgacgtgcc ggcaccccgc tgaccgggtc agtggtcgtc agccagtcgg
      781 tgagatttgc aagcggactt tcgccgcttc cggacggttt gaaattgctg gtttcgggta
      841 atagcaccgg cagctgctct tccggaagcg gaagggcttg gccatctacc cacacaatcg
      901 gaaagggttc gccccaatac cgctgacggg caaagagcca atcccgtaac ttgtagttta
      961 ctgccttgcg tcccttccca cgtgactcta accaggacgt aattttcgga atcgcgtcac
     1021 tcggcgccag tccatcgatg gtaaagcttc catccggcgt tgtggagttg acgacagtcc
     1081 ccttgtcggt tgcgacaaag gccgcctgtt ctacctgacc gccggcgatg acttcacgga
     1141 tcggcagatc ataggttttt gcaaaggccc agtcgcgctc gtcgtgcgct ggtacagcca
     1201 tgatggcccc ggtcccatag cccattaata catagtcggc aatccaaatg ggtagcggct
     1261 cattgttcac cggattcacc gcatacccgc cggtgaagac cccggtcttt tccttttcga
     1321 gttcctgccg ttggagatcg ctttttctgg acgccgcctc gcagtaggcg gtgaccaggg
     1381 tgagatattc agcagtcgtg acgaccttca ctaacggatg ttctggtgcc aacaccatat
     1441 aggttgcacc gaatagggta tcgggccttg tcgtatagac ccgaactgtt ccggtggcgc
     1501 cagccaaatc gaactcgact tctgccccga ttgatcggcc gatccaattt ttctgcattt
     1561 ccagcgtgct ggcaggccat tcgaccagct tcaaatcttc gaggagtcgg tcggcatagg
     1621 ccgtgatttt caatacccat tgacgcattg gcttgcggac cacatcgaac ccaccgactt
     1681 cactcttgcc atcgatgatt tcttcatttg cgagcaccgt ccccagtgcc ggacaccaat
     1741 tcaccggcac ttccgccacg taggccagcc cccgttcgaa caatttcaag aagatccact
     1801 gagtccagcg ataataatcg ggatcgatgg tgctgatctc acgctcccag tcgtaagaaa
     1861 ggccgatacg tttcatctgg cgtttgaacg tctcgatgtt ctgagctgtc gtgatggcag
     1921 gatggacccc ggtctttacg gcgtattgct ctgctggcag tccgaacgcg tcccacccca
     1981 tcgggtgcag cacgttgaat cccttcatcc gcttgtagcg ggacacgatg tctgtcgcag
     2041 tatagccttc caaatgaccc acatggagtc ccgaccctga gggatagggg aacatatcaa
     2101 ggcagtagaa ttttggcttg gtctggtctt cagccacacg gaaagtccgg tgctgctccc
     2161 aataggcctg ccatttctct tcaagggctt ggtgattgta ggttttgctc atacagcaac
     2221 cggctgccga tccatagaaa agagggagga ctctaacata gacctacggg agcaacaaga
     2281 atcgaggagg ggtttgtttg gttcaagcgg acgaagcgga tagacccggt gggtgagaca
     2341 ggccagatca tgacagaccg ctggaccttc cagcatatga ctaaatcacg ggccgtctgc
     2401 tgaatagcgg tgaaaaatct gtgaccgaga cgaactgggg gagcggggtg gggggcagtt
     2461 gagaactcga cttggcacta tgaatgagat gggcgactcc aaattctttt gctgcagtca
     2521 ggcaatgctc atcatcgtcc atgaagaagg atcttgccac gtcgaatccc agtaaccgct
     2581 ggcaactggg ccagtactcc ggacgcattt tcagataccc gacttcaaac gcgttcacaa
     2641 ttcgatcgac atgccggtcc agcccggtct tggcgacttt gatggctacg cccgattcat
     2701 gggcattcgt cacgatcgtc accctctttc ccagctggtg taggtgttgc agaaattctt
     2761 ccgcacccga aaggaatccg atcatgtgat ccaattcccg atgcaaggcg acgacatcga
     2821 ttcccacacg cctggtccaa taatgcagat cagtccaggc taactcgcct tcgactgagc
     2881 ggtacatttc cagtagtgtg tcgcgagatt cctcaagctg tagatcatga agtgcggcat
     2941 aacgacgggg cagctcctct tcgaaaaaaa agttgtcgaa atgccggtcg agcagagtcc
     3001 cgtccatgtc gagcaggaca tcgtcgatct ggctccagtc gacgacgtga ggggtaaggg
     3061 gtaaggggta aggggtcacg agcagagtat agcgaatgcc agggacgaac acgagagccg
     3121 ttgtctttgt cgaaactcct gtgt
//
LOCUS       DATMXI010000153         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_90879, whole genome shotgun sequence.
ACCESSION   DATMXI010000153 DATMXI010000000
VERSION     DATMXI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90879"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VN444_02885"
     CDS             <1..258
                     /locus_tag="VN444_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWR20791.1"
                     /translation="LGGMLVGSIVTGLFVAISMTSGGGAWDNAKKYIEEQGKKGTDTH
                     KAAVTGDTVGDPYKDTAGPAINPMIKVINIVALLIVSLIVK"
     gene            513..710
                     /locus_tag="VN444_02890"
     CDS             513..710
                     /locus_tag="VN444_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HWR20792.1"
                     /translation="MEKQERKQEPRREPQGKEEVKANPKVVEAGKKLKEDIDKLVDEI
                     DDVLETNAEEFVKNYVQKGGE"
     gene            906..>2414
                     /gene="recJ"
                     /locus_tag="VN444_02895"
     CDS             906..>2414
                     /gene="recJ"
                     /locus_tag="VN444_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWR20793.1"
                     /translation="MKSKLWVFRDINMSQRASLAQALLISPAAASLFLARGVTTPHEA
                     TRLMSLQSPHDPFLVPDMEQAIERLHRAVNTHEPICFYGDYDVDGITATSLYLSFFGG
                     LGAKVRAYVPHRLREGYGLNLGAVQRLHDEGISLLVTSDCGTTSHKEIELAAQLGMDV
                     VVTDHHQSDEVMPQAVAVLNPHRTDTLYPFRGLCSAALAYKVAQAYQVRYGVAGVPLE
                     SLLDLVALATVADVVPLQDENRSFVREGLHQLSRGARCGIRALKQAAGVTRDCTAETI
                     AFKLAPRINAAGRLDDAMLGVRLLTTEIPSEAQQLADRLEQLNHERQRIEMDIMTEAL
                     ASLKDSALPRVLVLASRHWHLGVVGIVAARLVDRFQCPAIVMAINEQGIAKGSARTTG
                     GFDLYQGLAACREVLESFGGHPSAAGLTIRESRIDEFRAMFSDVVAGWVHDGYKVPTL
                     NVDAEVRLNEVNLQLIQEIGSLHPFGAGNPEPTFAVTGLEVMESRTVGEKHLK"
BASE COUNT          511 a          612 c          736 g          555 t
ORIGIN      
        1 ttgggcggca tgctcgtcgg cagtatcgtc accggtctgt ttgtcgccat ctcaatgacg
       61 agcggcggcg gtgcctggga taatgccaag aagtatattg aagagcaggg caagaagggg
      121 acggataccc acaaggcagc agtgactggt gacacggtcg gcgatcccta caaagatacg
      181 gctggtccgg cgattaatcc gatgatcaag gtcatcaaca ttgtggcctt actcattgtg
      241 tcgctgattg tgaaatagcg agacgcgctg gacgtgcgag aaaagcggga cgagccagac
      301 ggaagagact tcgaggttgt cggaactgtg gatcctgaat ctcgaatttc gtgtcgcgcc
      361 tgtcgcgcac atctcgcttg tcacgctcct gcctcgcttc ccgtttccag gctcggcttg
      421 acggctgaaa gaatcgtgag gtaaggtgtt cgtacgggat catgctggtg gctgatccac
      481 gagagttccc cagattcacg ggaggtgctc ggatggagaa acaggagcgt aagcaggaac
      541 ccaggagaga gccgcagggg aaggaagagg tcaaggccaa tccgaaggtc gtcgaagccg
      601 gtaagaaatt gaaggaagac atcgataagc tggtcgatga aatcgacgac gtgcttgaaa
      661 ccaacgcaga agaattcgtc aaaaactatg tccaaaaagg gggggaatag ccacggctgc
      721 cctgtggcct attctctcct gttccttcct cctcatacta ctccggtgct cagggagcgc
      781 ctctgtccct gttcagcatg ccattcgatt tctccggttt gacaacgcag cactgagcgg
      841 ctagtatccc cttaattgtc agatagttag cttctcttga gactcaggac ccttgaagag
      901 gccttatgaa gtccaagctc tgggtcttcc gagacatcaa tatgtcccag cgggcttcat
      961 tggcccaggc cctattgatt tcacctgccg ccgcatcact gtttctcgct cgtggtgtga
     1021 ctacgccaca tgaagcgacg cggttgatgt cgctacagtc gccgcatgac ccgtttttgg
     1081 ttcccgacat ggaacaggcc attgagcgac tgcatcgggc tgtgaatact cacgagccga
     1141 tttgtttcta cggcgactat gacgtcgacg ggattactgc gaccagtcta tatctgtcct
     1201 tctttggagg tttgggagca aaggttcgcg cctatgttcc acatcgtctc cgcgaaggct
     1261 atggacttaa tctcggcgca gttcagcgat tacatgacga aggcatctcc ctcctggtga
     1321 catctgattg cgggacaact tcgcacaaag aaattgagtt ggcggcccag ctgggtatgg
     1381 atgtggtagt gacggaccat caccagagcg acgaggtgat gccacaggct gtggcggtgc
     1441 tcaaccccca tcgaaccgat accctttatc ctttccgtgg cctttgttct gcggcgctgg
     1501 cctacaaagt ggcgcaggcg taccaggtac ggtacggggt cgctggtgtg ccgttggagt
     1561 cgctgctcga tctcgtggca ttggccacgg tcgcggatgt tgtacccttg caggatgaga
     1621 atcgaagctt tgtgcgcgaa ggcttgcatc agttgtcgcg tggcgcccgc tgtggcattc
     1681 gcgcactcaa gcaggcggct ggcgtgacgc gcgactgtac ggcggagaca attgccttca
     1741 aactggcgcc gcgcatcaat gctgcggggc gattggacga tgctatgctg ggtgtgaggt
     1801 tattaacgac cgaaattccg tccgaagcgc agcaattggc agacaggctt gaacaactga
     1861 atcatgagcg gcagcgtatc gagatggata tcatgacgga ggcactggct tcgcttaaag
     1921 attccgcatt accgcgcgtg ctcgtattgg cttcccggca ctggcatctt ggggtggttg
     1981 gtatcgtcgc ggcacgcctg gtcgatcggt ttcaatgccc ggccattgtc atggctatca
     2041 atgagcaggg tatagcgaaa ggctcggcca ggacgacggg cgggttcgat ctctatcaag
     2101 gattggctgc ttgtcgagag gtgttggaat cgttcggggg acatccgagt gccgcaggtc
     2161 taactattcg cgagtctcga atcgacgagt ttcgtgccat gttttccgac gtggtggccg
     2221 ggtgggtgca tgacggctat aaggttccga cgttgaatgt cgatgctgag gtgcgtctga
     2281 atgaggtgaa tctccagttg atacaggaaa tcggctcgct gcacccgttc ggtgcgggca
     2341 atccagagcc gacgtttgcg gtgaccggtc tcgaagtcat ggagtcacgc accgtcggtg
     2401 aaaaacactt gaaa
//
LOCUS       DATMXI010000154         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_23787, whole genome shotgun sequence.
ACCESSION   DATMXI010000154 DATMXI010000000
VERSION     DATMXI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23787"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..631
                     /locus_tag="VN444_02900"
     CDS             419..631
                     /locus_tag="VN444_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20794.1"
                     /translation="MAKNTADESDETRLKKKIAARGADHKGSQEGGALRSLRKRLKRT
                     QRKRRSLALRKKNSASKKTTAPEAKK"
     gene            682..1500
                     /locus_tag="VN444_02905"
     CDS             682..1500
                     /locus_tag="VN444_02905"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWR20795.1"
                     /translation="MEQLQKKEERTNPDGSELVTDELTDQVAADFTGEQAGRVEPVTA
                     TTELVLLEEEKVKDEIEIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLCHALRDGD
                     WQVREVAIEGLGQIGSPAVELLIRLLRDWDVRKYAIAALGKIRDERVLDPLMLQLRND
                     EFKDDALNALVELGQPALSRLIKALKDKDENIRKQAVLALGRIKEAEAIEPLIEMLGD
                     GDWFTRLTAAAALEAIGDERGREAIKPLMKDPDMVVRMRVERILAKWKKQSATA"
     gene            complement(1512..1967)
                     /locus_tag="VN444_02910"
     CDS             complement(1512..1967)
                     /locus_tag="VN444_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20796.1"
                     /translation="MWKFVAVLVLIAASFGVGFYFGQRPIGTLEETVSGLQHSLKDTS
                     RNIIDTTMGIERDLRRRQGLIDSKSRVVQAKSELLDKNFGEAAKQLGEAAETLETVVK
                     GAKQDESILALRALTGTLQEVRLELSMGKPVSMKKLDEIQLEVDRQLNK"
     gene            complement(2094..>2356)
                     /locus_tag="VN444_02915"
     CDS             complement(2094..>2356)
                     /locus_tag="VN444_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWR20797.1"
                     /translation="PSIRAAAAHTLSATSDKQAVAILQTLLQDQQPQPRLAAVKALGK
                     SGAPVTPLLKQGLQDSDVAIRLAAAGSLLQQLRRPTTAPKAR"
BASE COUNT          561 a          610 c          690 g          495 t
ORIGIN      
        1 gtactggcca ccatcgaaca gccccacaaa atccctgtca gcaatagccc ggacccatag
       61 cgaagccaca tcgtcttcac ctgtatcaag ccttggtctc agtgtctttg ctaataagag
      121 aagcatagca tatggtctgg aatttgccgg atcggaggca ggccctctca tgagaaatac
      181 catagcgaga tcactgggat acaaaggggg agggcgccag tacttttaaa aggcagtgaa
      241 gcatggcgtc acagtcaggc agcctgcgac cttcacgagt tcattggaag atctgttccg
      301 aagtcggggt atgggctcat gttgacaggc tgcgaagcgc tccactacac tgcttccttg
      361 ctctggggac taaaaggcga attcaatcaa tcaggtatgc agggaggccc gtatccggat
      421 ggcgaaaaac acggcagatg aatcggacga aaccagactg aaaaagaaga ttgcagcgag
      481 aggcgctgac cacaaaggct ctcaagaggg tggagccctt cgctccctca ggaaacggct
      541 gaaacggaca cagcggaaac ggcgttccct ggccctgcgc aagaaaaatt ctgcgagtaa
      601 gaaaacgacc gcccccgaag ccaaaaagta atgtccctgt cgggacaatc gattggcgag
      661 tgacgtatcc gggtgataca gatggaacaa ctccaaaaaa aagaagagcg gacgaatcct
      721 gacggttcag agttggtgac cgatgagttg actgaccagg tggcggccga ttttaccgga
      781 gagcaggcgg gacgagttga accggtaaca gcgacgaccg agctggttct tcttgaagaa
      841 gagaaggtca aggatgagat cgagatccaa atcgatctgc tcaaggatcc cgactgggtg
      901 gtccggcgcg aggccgccat cacgctcgga gagatgggcg atgaacgttg tgtggaaccc
      961 ctgtgccatg ccttgcgcga cggcgactgg caagttcgtg aagttgcaat cgaagggttg
     1021 ggacagatcg gttctccagc cgtcgaactg ttgatcaggc tgctccggga ttgggacgtt
     1081 cgcaagtacg ccattgccgc gctagggaag attcgtgacg aacgtgttct cgaccccctc
     1141 atgcttcaac tgcgcaacga cgagttcaaa gacgatgcgc tcaacgcgtt ggtcgagctg
     1201 ggtcaaccgg cgttatcgcg attaatcaag gcgctgaaag acaaagacga gaacattcgg
     1261 aagcaggcgg tgctggcttt gggccggatc aaggaggctg aagcgatcga gcctctcatc
     1321 gagatgctcg gcgatggtga ttggtttacc aggttgacgg ccgctgccgc gctcgaggcc
     1381 atcggcgatg agcggggtcg cgaggccatc aaaccgttga tgaaggatcc ggatatggtg
     1441 gtgcggatgc gggtagagag gatcttggcg aagtggaaga aacagtccgc aaccgcgtag
     1501 caaaagtcag cttatttatt cagctgccgg tcaacttcca gctgaatctc atcgagcttc
     1561 ttcatcgaca ccggtttccc catcgacaat tccagccgta cttcctgaag cgttccggtg
     1621 agtgcccgta gggcgaggat cgactcgtcc tgctttgcgc ccttcaccac cgtctccagc
     1681 gtctcggcag cctcacccaa ttgcttagct gcctctccaa agttcttatc gagcagttct
     1741 gattttgcct gtaccactcg agacttgctg tcgatcagtc cctgtcgcct gcggaggtct
     1801 cgctcgattc ccattgtggt atcgatgata ttgcgcgatg tgtctttaag cgaatgttgg
     1861 aggcccgaga ccgtctcctc cagtgtcccg atcggacgtt ggccgaagta aaatcctaca
     1921 ccgaacgacg cagcgatcaa gacaagaact gcgacaaatt tccacatcac gcactccttt
     1981 cctctaacaa ggcggtgcaa actacttaag tatgacagaa atgcgatgtg tagacgactc
     2041 acagagtcgt tcttcactct tccaaggtgc tggcggatgt tatcaacagc ttattagcga
     2101 gctttgggag cagtcgtggg gcggcggagt tgttggagaa ggctgccagc agcagccaga
     2161 cgaatggcga cgtctgaatc ctgtagccct tgtttgagca atggcgtcac cggtgctcca
     2221 ctcttaccca atgccttcac cgcagcgagc cgtggttgtg gctgctgatc ttgcaggagc
     2281 gtttgtaaaa ttgctaccgc ctgcttgtca gatgtggccg agagcgtgtg ggccgctgca
     2341 gctcggatgg aggggt
//
LOCUS       DATMXI010000155         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_268387, whole genome shotgun sequence.
ACCESSION   DATMXI010000155 DATMXI010000000
VERSION     DATMXI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268387"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..486
                     /gene="tatB"
                     /locus_tag="VN444_02920"
     CDS             187..486
                     /gene="tatB"
                     /locus_tag="VN444_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HWR20798.1"
                     /translation="MFGLGIGEVLIILVIAFLLFGPKQLPEVARQVGKMVKGFKETAE
                     DLRKSVEPELNMIQQEIKTVEQDFQASIKDAEEQITAATKPAEQQADSKGSANQT"
     gene            934..2583
                     /locus_tag="VN444_02925"
     CDS             934..2583
                     /locus_tag="VN444_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWR20799.1"
                     /translation="MVATGMSFETSPAQAAFELPAGEKITHTAVVARAIPDKEAYELY
                     DPKIGKNFDINKFWMRADLRVRYEMRNNICFGSAMAGNTLGACNTFNGQTSANGTPGK
                     ANDSYAMQKARLGFGYDLSPDVNFYMELQYASVWGANGLQGTPVQDNQRTQNGQNCAT
                     QPCWGNGGVLGVRAAYMLIRNFAGAEGLSVKAGRQYIVFGNHSIFGAFDWSNTGFSFD
                     GIMLQYSTKAWDSYAGWFRTSESDLEQGAPLGALHNNLPVSGSDNPTLNNGSANIDSD
                     VFLFYNQIKSVPGFVIEPYYILYSNRYNSGDNRNQGLGTPKHSNQTRHMIGNRIELRK
                     GNIDGWNETIWQFGQMGDNGGANAGYGNQKNLSINAWATRTWIGYTHYEWEWKPRLAF
                     NFDYASGDGRANCTLPGGEATGCKTANTFENLWGTNHIHMGYMDTMGWKNMMKPSINL
                     QFRPTKDDHIEIWATSLNLANTKDNWYRASQTVYVYSKTNNTTRHVGNELDIIWTRFF
                     MDGKLSFQAGYGHLFAGDYIEQNLGTSTDQDWAYAQLWVNF"
BASE COUNT          665 a          721 c          713 g          546 t
ORIGIN      
        1 cgcctgaagc gccaccctcg caggtagcgc aggccgctcc gccggccacg gcgcctgttc
       61 aatcagggac gcaggtcaag caagggtaga acgtcgtgaa cagtgatgtg tgatgcgtga
      121 tgagagaagg atctcgaaag cgatcactga tcacccatca ccgatcattc atcactgggt
      181 tgcaacatgt tcggattggg catcggcgaa gttcttatta ttttggtgat tgcattcttg
      241 ctgtttggcc ccaagcagct gcctgaagtc gctcgccagg tcgggaaaat ggtcaagggg
      301 ttcaaggaga cagcggaaga cttgcggaag tcggtggagc cggaactcaa tatgatccag
      361 caggaaataa agacggtcga gcaagacttt caagcttcaa tcaaagacgc agaagagcag
      421 atcactgccg caacgaagcc ggcagaacaa caagctgatt caaaaggatc tgccaatcag
      481 acctagcggt acagaatccg gtctagagat accttccaga gtttttctag gttactctcc
      541 ccttcacgaa ccagttgcat tctctccccc acatcattag tattggcgcc acaaaaattt
      601 ccccatcgat ttcgtgcccg ttagaaatgt gaatcactca atcagcgtgt gaggctgagc
      661 gtcttacaaa agagccggcg tgaagccgct tcatgctacc ccagccgcag tcgtcttttg
      721 tgtcatccgc ctgaaggata gggctatcta tatgatggcc gtatcctctc ccgtagcgtc
      781 ggcagatttc caaagaccag agaattcggc tcacgggtcc ggggcaggtc agtagacggc
      841 ctcccctagc gactaccgct ggagaggaga acccttgtga gaatcatcgg gcagatagga
      901 caggccagtc ttgctattct ctgctgtgcg gccgtggtcg caacagggat gtcattcgag
      961 acgtccccgg cacaagcggc atttgaattg cccgcgggtg aaaaaatcac ccatactgcg
     1021 gtcgtggctc gagcgattcc ggacaaagaa gcctacgaac tctacgaccc gaagatcggg
     1081 aaaaactttg acatcaataa attctggatg cgtgccgacc ttcgcgtgcg gtatgaaatg
     1141 cggaacaata tttgcttcgg gagcgccatg gctggtaata ctttaggagc ctgcaatacg
     1201 ttcaacggcc aaacctctgc gaacggtact cctggtaagg caaacgacag ttacgccatg
     1261 cagaaggcac gtttaggttt cggctatgac ctctccccgg acgtgaattt ctacatggaa
     1321 ctccaatacg catccgtgtg gggtgccaac gggctacagg gtaccccggt ccaagacaac
     1381 cagaggactc agaacggcca gaactgcgcg acacagcctt gttgggggaa cggcggcgtc
     1441 ctgggcgtcc gagccgccta tatgttgatc cggaactttg ccggggccga gggcctgagc
     1501 gtgaaggccg gccggcaata tatcgtgttc ggcaaccact ctatattcgg cgccttcgac
     1561 tggtccaaca caggattttc ctttgacggt atcatgttgc aatacagcac caaggcctgg
     1621 gatagctacg ccggttggtt ccgcacttct gagtccgatc tcgagcaagg agcaccgctc
     1681 ggcgctctcc ataataacct tcctgtcagt ggtagcgaca accccacatt gaataacggc
     1741 agcgcgaaca tcgattccga cgtgtttctc ttctacaacc agatcaagtc ggtgccggga
     1801 ttcgtgatcg agccctacta catcctctat tccaaccggt acaactcagg ggacaaccgg
     1861 aaccagggtc tcggcacacc gaagcactcg aaccagactc gtcacatgat cggaaaccgg
     1921 atcgagctgc gcaaaggcaa catagacgga tggaacgaga cgatctggca gttcggtcaa
     1981 atgggtgaca acggtggagc caacgcaggc tatggcaatc aaaagaatct cagcatcaat
     2041 gcctgggcca ccaggacctg gatcggatac acacattacg agtgggaatg gaaaccccgg
     2101 ttggcgttca acttcgacta tgcctccggc gatggcaggg caaactgcac gctccctggc
     2161 ggtgaggcca caggttgtaa gactgccaac acgtttgaga atttgtgggg gaccaaccat
     2221 atccacatgg gctatatgga taccatgggc tggaagaaca tgatgaaacc ctcgattaac
     2281 ctccaatttc gcccgaccaa ggatgaccac attgaaattt gggccaccag cctgaacctg
     2341 gccaatacga aggataactg gtatcgcgcc tcccaaactg tctatgtcta ttcgaagacg
     2401 aacaacacca cgaggcacgt cggcaacgaa ttggatatca tttggacgcg ctttttcatg
     2461 gatggcaagt tatccttcca agccggctat ggtcacctct tcgccggcga ctatatcgag
     2521 cagaacctgg gcaccagcac cgatcaggat tgggcctatg cccagctctg ggtgaacttc
     2581 taagcaggag atgggaaggg taacctagtc gtcctcctgc tcgcggaacg cgcacgatag
     2641 gaatg
//
LOCUS       DATMXI010000156         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_179826, whole genome shotgun sequence.
ACCESSION   DATMXI010000156 DATMXI010000000
VERSION     DATMXI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179826"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VN444_02930"
     CDS             complement(<1..995)
                     /locus_tag="VN444_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR20800.1"
                     /translation="MLPNLLEKLGPYVSQALGLPTESLLQAFRPAIEGDLPGILELRQ
                     AVLRDALWWDDEAFVRWRYFDNKSATEPTPYWVFEKEKEIIGGIGLEPLVLVVDGEPR
                     PAVRTLDIMVRPDFDGQGLGVLMNLVIFKHFPITLVTGGNERSRNLISRLFRHALDLH
                     VWKAIIKSRERVERRLGPGPVASAVAMVGDLFLAMDRRNWRHPLPKKLEIRELTRFDS
                     WVMVLSQEFERTGRILVRRSPEYLNWRFVRNPRCRYRILGGLVGGKIVGYVVTRFNLS
                     RPNPRKEAEIVDCLALPTIGGEISPLPFLIRAAVDQLVREGACLVSCTAFDDAIQT"
     gene            complement(1035..1310)
                     /locus_tag="VN444_02935"
     CDS             complement(1035..1310)
                     /locus_tag="VN444_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWR20801.1"
                     /translation="MATERRDSTDAVSVDEALAWVAEMFEEPRENVRENTPRDDIAAW
                     DSLGQLVLMAKLDERFGIRLTQADLRSLQSVRAILDVLCANGRLNGV"
     gene            complement(1642..2415)
                     /locus_tag="VN444_02940"
     CDS             complement(1642..2415)
                     /locus_tag="VN444_02940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR20802.1"
                     /translation="MTLVKQSFLDFSEKWVVVTGASAGIGQACALELGKHGARLVLVG
                     RNESALAKTFSLLDGEGHEVLRLDLSDLPKISAEVLRIAKKVGRLYGLCHAAGITATS
                     PLSTSTFDVIQPMMTVNVLAGLELARIVTRRDVMEPEGGSMVFLSSIYGRGGVAGETG
                     YCATKGAITAAVRAMAIELARRRIRVNSISPGLVKTPMTDKALSVLNSAQVTAIEEKH
                     PLGPGTALDVARAAVFLLAPATSWITGIDLAVDGGYSAQ"
     gene            complement(2412..3467)
                     /locus_tag="VN444_02945"
     CDS             complement(2412..3467)
                     /locus_tag="VN444_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HWR20803.1"
                     /translation="MMAQSVITGASIKGLATCVPARRFNNLTDTTAFTKDEVRKVVAM
                     AGVTERRVVDSNTCSTDLCFAAAESLLEMVEWDRASIDGLIMVTQTPDYFMPSSSCVL
                     HKRLKLSEHCAAFDLGLGCSGYIYGLWLASMMVKTGGLRRVLLLHGETPTLYTHESDR
                     AVSLLFGDAGSATAIEGSSTAESTDWYFTLQTDGTGYDDLIIEGGGFRDRFCGDIRSH
                     YVKMNGANVFNFTIKVIPPLIEDILRLANRRPEEVDYYVFHQSNRFIIKYLMGGCALP
                     PEKVPIILDRYGNPGGPSIPLTITQGIAPRPGRHRLWLMLLGYGVGLSWGAALIPFGA
                     DAAVRHVEWPSSEARTA"
     gene            complement(3619..3906)
                     /locus_tag="VN444_02950"
     CDS             complement(3619..3906)
                     /locus_tag="VN444_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20804.1"
                     /translation="MAMLVTWPVFVPAYFLQSLGASLWIAGFIHGGVPAALMVLAFYY
                     SDLYAVDQTASAGEMLLRLMNDFGLFCPVVGEQRLSFRSWDPEKCISARRC"
BASE COUNT          965 a         1317 c         1156 g          868 t
ORIGIN      
        1 gtctggatgg catcgtcgaa ggcggtgcag ctcacgagac aggcgccttc ccggacgagc
       61 tggtcaacgg ccgctcgaat aagaaacggc aaaggggaga tctcgccgcc gatggtgggt
      121 agggcaagac aatccacgat ctcggcctcc ttcctggggt tgggcctgga caggttgaag
      181 cgcgtgacca catagccaac gatcttcccc ccaaccaatc ccccaaggat ccgatagcga
      241 cacctcgggt tacgaacaaa tcgccagttc aagtactcgg gactgcgccg caccaggatc
      301 cggccagtcc gctcgaactc ttggctgagc accatcaccc atgaatcgaa ccgcgttagc
      361 tcgcgaatct ccagcttttt cggcaatgga tgtcgccaat tccgccggtc catcgcgaga
      421 aagagatcgc cgaccattgc aacagcactg gcgactggac ccggacccag tcggcgttcg
      481 acgcgctcgc ggcttttgat gatcgccttc cagacgtgaa ggtcaagggc atgacggaac
      541 aaccgagaga taaggttgcg tgagcgctca ttgcctcctg tgaccagggt aatagggaaa
      601 tgcttaaaga tcaccagatt catcagtacg cccaggcctt ggccgtcgaa gtcaggccgg
      661 accataatat cgagcgtgcg aacagcaggg cggggctcgc catcgacgac cagaaccaag
      721 ggctccagcc ctattccccc aatgatctct ttctctttct cgaaaaccca gtaaggtgtg
      781 ggttccgtcg cgctcttatt gtcgaagtac cgccaacgga cgaacgcctc gtcatcccac
      841 caaagggcgt ccctgagaac agcttggcgc agctcaagga tgccgggcaa atctccctcg
      901 atggcgggac ggaaggcctg gaggaggctc tcagtaggaa gacccaaggc ctggctcaca
      961 taagggccaa gcttttcgag taagttgggc agcatctgaa ttagtcagac atgccaatgg
     1021 gcaacgcaaa gattttacac accattgaga cgaccgttag cgcaaagcac atccaaaatg
     1081 gctcgtacgg actgaagcga acgcagatcg gcttgggtca ggcgaatacc gaagcgctcg
     1141 tctagcttag ccatcagaac caactgaccc aaagagtccc aggccgcaat atcatcgcgg
     1201 ggcgtgttct cccgcacgtt ctctctgggc tcctcaaaca tctctgccac ccaggccagc
     1261 gcctcgtcaa cgctgactgc atcagtggaa tctctacgtt cagtcgccat tcgccaatct
     1321 catgatcagg tcgttttctg ggtctctcgc tgcttgaagc gggtaaagcc ttcgagtata
     1381 ctctctggga gaacaggcta tttggttact caccctcatg cgaggaacca acaatgtgaa
     1441 tactaatata actggcagcc attccttctg caatagccca ttaggcgtat ttttcgagac
     1501 aacgtggcct agacctacgc cctgagatcc aatcgctatc agcccgagca acgagcacaa
     1561 ttcctactca actttgtctt acccacataa aatagcgagg aaccgttttc tgtccgttcg
     1621 tcccgtatgg ggaaacgcgg ctcactgcgc tgagtagcct ccgtcgaccg caaggtctat
     1681 acctgtgatc cagctcgttg cgggggcgag taggaacact gcggcccgcg cgacatccaa
     1741 ggctgttccc ggcccgagtg ggtgcttttc ttcaatcgct gtcacctgcg cgctattgag
     1801 aacgctcagc gctttgtccg tcatcggcgt cttcaccagc cccggagaaa tggagttaac
     1861 gcgtattctc cggcgtgcga gctcgatcgc catggcgcgt actgctgcag tgatggcgcc
     1921 cttggtagcg cagtatccgg tctcaccggc tactccaccc ctcccgtaga tcgatgagag
     1981 gaacaccatg ctgcctccct ccggttccat cacgtctcgc cgagttacga ttctcgccaa
     2041 ttccagaccc gcgaggacat tgaccgtcat catcggctgg atgacgtcga acgtgctggt
     2101 actgagggga ctggtcgccg taatcccggc ggcatggcaa agcccgtaaa gtctcccaac
     2161 ttttttcgct atgcgcagga cttctgcgct tatcttcggc aaatcgctca ggtccaagcg
     2221 caaaacctcg tgcccctccc catcgagcaa cgaaaaagtc ttcgccaagg ctgactcgtt
     2281 ccgcccgacg agcacgagtc tggcgccatg cttgcccagc tccagcgcgc atgcctgacc
     2341 gatacccgcg gaggcgccgg taacaactac ccacttttcg gagaagtcca ggaaagattg
     2401 cttcaccaac gtcatgctgt cctcgcttca ctcgacggcc actccacgtg acgaaccgcg
     2461 gcgtcagccc cgaacgggat cagggctgcc ccccaggaca gcccgacgcc gtagccgagc
     2521 agcatgagcc ataacctgtg ccttccaggc cgcggcgcaa tcccctgcgt aatggtcagc
     2581 ggtatcgaag gtccgcccgg attcccatag cgatccagga tgatcggcac tttttcaggt
     2641 gggagagcgc acccgcccat caagtacttg ataatgaagc ggttggactg gtggaagacg
     2701 taatagtcca cttcttccgg ccttcggttg gccaagcgca ggatatcctc aatcaagggc
     2761 ggaatcactt tgatcgtgaa gttgaagaca ttggcgccgt tcatcttcac atagtgagac
     2821 ctgatgtcac cgcagaaacg gtcgcgaaat cctccgccct cgataatcag gtcgtcgtaa
     2881 cccgttccgt cggtctgaag cgtgaaatac cagtcagtcg actctgctgt ggaactccct
     2941 tcgatcgcgg tagccgaccc cgcgtcgccg aagagcaggg acacggcccg gtcgctttca
     3001 tgcgtgtaga gagtcggcgt ttcgccgtgc aacaagagca cgcgcctgag accaccggtc
     3061 ttgaccatca tcgacgccag ccagaggccg tagatgtaac cggagcagcc caagcccaag
     3121 tcgaaggccg cacaatgctc agacagtttg aggcgtttgt gtagcacaca actggaggac
     3181 ggcatgaagt aatcgggggt ctgtgtgacc atgatcagcc cgtcgatcga ggctcgatcc
     3241 cattccacca tctccaggag agactcggcg gccgcgaagc acaggtcggt gctgcacgtg
     3301 ttgctgtcga caacccggcg ctcggtgacc ccagccatcg cgacaacctt ccgaacttcg
     3361 tccttcgtga aggccgtcgt gtccgtcagg ttgttgaatc tgcgcgcggg aacacaggtg
     3421 gccagacctt tgatcgaggc gccggttatc actgactgag ccatcattca gacccaacgc
     3481 gaagaaaatg ccggagttat cttcttcatc gtatccgttt ccaaccgcgg cccgacaaag
     3541 cccatgtccc ctttgcggat attaatcatc tgagggaact cgtcgatccg gagcgccaga
     3601 ggaaaaggcc gatctccatc agcaccgcct cgccgagata cacttttctg gatcccaact
     3661 ccggaatgag agccgttgtt cccccacgac cggacagaac aggccgaaat cattcatcag
     3721 gcggagcagc atctctccgg ccgaggcagt ttgatcgacg gcgtagagat ctgagtagta
     3781 gaaggcgagc accatcagcg cagccggcac ccctccgtga ataaagcccg caatccaaag
     3841 agaggcgccc aacgactgga ggaagtacgc cggaacgaag acgggccacg tgaccaacat
     3901 cgccacgccg acatcaccca gtaggagcac taagtcgcgt aggctgagta gtagcgattg
     3961 aagatcatcc tgactctacg tttacagaac gccgaacggc ctgaagattt tttgtttgtc
     4021 tgataaggta ttacttttga tgggggaagt ataagcagcg acggacttgt gtgtcaaggc
     4081 aatattgcga tcagcggtcg gctcttggct ggaggctaaa cgcattgggc tgatacctcg
     4141 ttctatcatg agcaccatac agcataaggc ccccctgaag caacaggagg gcctctactt
     4201 ctgcctgcgc ttcggcttct acacccttgc ttaccttcct tgccctctat gccgtgcatg
     4261 agccgctcgt cgtcccctgc acctgcctgt cagcgcgctt caggat
//
LOCUS       DATMXI010000157         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_5241, whole genome shotgun sequence.
ACCESSION   DATMXI010000157 DATMXI010000000
VERSION     DATMXI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5241"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VN444_02955"
     CDS             complement(<1..349)
                     /locus_tag="VN444_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWR20805.1"
                     /translation="MAYQALIHTVKGSVATITLNRPDRYNALNKQMVEELLEVVIACQ
                     EDRAVRAVVLMGAGQAFCAGGDVREFIEHTSTIALYVKRLLMSLHSVITCICRMSKPV
                     IAGVSGVASGAGMS"
     gene            complement(384..1136)
                     /locus_tag="VN444_02960"
     CDS             complement(384..1136)
                     /locus_tag="VN444_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWR20806.1"
                     /translation="MNEEKRLALFIDFENIAIGIKQAKKQFEVGLVLERLVEKGTIMV
                     KRAYADWGRYAEYKRHLHEAAIELIDIPQKRISGKNSADIRLAVDAMDMAWSKEHLNT
                     FVIVSGDSDFSPLVSKLRENNKEVIGLGVKNSVSELLVDNCDEFIYYEDLIRSPKKPP
                     VIAGLPEKKAEAFDLLSDSIQALMRENKEVLWGSMVKQTMIRKRPSFNEGYYGYSTFS
                     KLLEDAAKHNIIELRRDPKSGTYIITSFAEGA"
     gene            complement(1353..2558)
                     /locus_tag="VN444_02965"
     CDS             complement(1353..2558)
                     /locus_tag="VN444_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HWR20807.1"
                     /translation="MSVKLRLIAAILLPLQWLSIRLFDVHLPPQWEVVGAGLAIFGAA
                     FLLSWAAEVAQVDIPQALALAFLALVAVLPEYAVDIYFAWSAGKDPTYIAYATANMTG
                     ANRLLIGVGWASVVMTFWFKTRHRQVVLDRSRYIELLHLVLATIYSLIIPLKGTLSAL
                     DSFILVAIFVSYMISASKAQIIEPELDGGVGELLMQLRPNLRRSATIAMFVYAGLGIF
                     LAAEPFAEGLLATGRIFAIEEFLLVQWLAPLASESPEFIVAIVFALRGNPGASMGTLL
                     SSKVNQWTLLIGTLPLAYNLSAGHLGAMHLDARQTEEIFLTAAQSLFAIAVLANLSFS
                     LAEAGLIFVLFGTQLFFTDPISRHLYAIGYIVLAVAWFIVSRSSRQGLAAMVVERWTG
                     KGIATSQQK"
     gene            complement(2602..3345)
                     /locus_tag="VN444_02970"
     CDS             complement(2602..3345)
                     /locus_tag="VN444_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWR20808.1"
                     /translation="MKKTVMIATVLIAVAGLASAYALFNEWLDLGRYWEWIEPSANEQ
                     ALARYIKQSGAWAPLVFIGIQALQIVVAPIPGEVTGIIGGYLFGTFSGLLYSTIGLTI
                     GSCLAFSMGRWLGHHFARRFVTPKTYEMFFFLTRTQGKLITFLLFLIPGFPKDVLCYI
                     LGASPLSFGAFFLLTAVGRLPGTWLLSMQGRQVRTAHYGSLVALIFFLSVACLLLYLY
                     REPLFRWIKLDHYRRQRRKRDIVKDQEVA"
BASE COUNT          842 a         1068 c         1068 g          743 t
ORIGIN      
        1 agctcatccc ggcgccggag gccaccccgc tcaccccagc gatgaccggc tttgacatcc
       61 gacagataca tgtgatcaca ctgtgaagag acatcaacag ccgttttaca tacagggcga
      121 tggtgctagt atgttcgatg aactctctga catccccgcc ggcgcaaaag gcctggccgg
      181 cgcccatcag cacgactgcg cgaactgccc ggtcctcctg gcaggcgatg accacctcca
      241 gcagctcctc caccatctgc ttgttcaggg cattgtaacg atcaggacgg ttcagggtga
      301 tggtggccac cgaacccttt accgtgtgga tcagtgcctg ataggccatt ctgcccccac
      361 caaagccttc agccttcagc ctcttatgcg ccttccgcga agctggtgat gatatacgtc
      421 ccgctcttag ggtcccgcct cagctcgatg atattgtgtt tggcggcgtc ttcgagcagc
      481 ttggaaaatg tgctgtaccc gtagtaccct tcgttaaagg agggccgttt gcggatcatc
      541 gtctgcttga ccattgaccc ccagagtacc tctttgttct ctctcatgag ggcctggata
      601 gagtcgctca gaaggtcgaa cgcctccgcc ttcttctcag gcagaccggc tatgacgggt
      661 ggcttcttgg gggagcgaat caggtcttcg tagtagatga attcgtcgca gttgtccacc
      721 agcagttccg agaccgaatt cttcacgccg agaccgatga cctccttatt attctctcgc
      781 aatttggaga ccaggggcga aaaatcgcta tcaccagaga cgatgacaaa ggtgttcagg
      841 tgctccttgg accacgccat atccatcgca tccaccgcca gccggatatc ggcgctattc
      901 ttgccgctga ttcgcttctg tgggatgtcg atcagctcga tagccgcttc gtggaggtgc
      961 cgtttgtact cggcatagcg tccccagtcg gcgtaggcac gcttgaccat aattgttccc
     1021 ttctccacga gccgctcaag cacaagaccg acctcgaact gcttcttggc ctgcttgata
     1081 ccaatcgcga tgttctcaaa gtcgatgaag agggcgagtc tcttctcttc gttcattgaa
     1141 cccctttctg cgtgacgccc ttcaggccgc gccgatggca tgacggatga tctgcatttc
     1201 ccacagtacc ccgttggctt aagataagcc taaacatgcc acctgtagcc tgtcaacagg
     1261 tttaaacgga ctgagtctct gcttgggagg caccactccc atccccagat cagctcttcg
     1321 gccgtcacct accctcgtcg gatcgtctct tttcatttct gttgggaagt tgctatgcct
     1381 tttccagtcc atcgctccac caccatggcg gcaaggcctt gccggcttga gcggctgacg
     1441 atgaaccacg ccactgcgag cacaatgtaa ccgatggcat agaggtgacg ggaaatagga
     1501 tcggtgaaga acagttgagt gccaaagaga acgaagatga gaccagcctc tgccagagag
     1561 aacgaaagat tggccagcac ggcaatcgcg aagagggatt gagccgctgt cagaaatatc
     1621 tcctcagtct gcctggcatc caggtgcatc gccccaagat ggcccgcaga aaggttgtag
     1681 gcaagcggga gcgtgccgat cagaagggtc cattgattga ccttggaaga taggagcgtg
     1741 cccatgctgg ccccggggtt cccacgaagc gcgaagacga tcgccacgat gaactcaggc
     1801 gactcagagg cgagcggcgc cagccactgc accaggagaa actcctcgat cgcgaatatc
     1861 cggccggtgg ccaaaaggcc ctcggcgaag ggttcggccg cgagaaagat accgagcccg
     1921 gcatagacga acatcgcgat ggtagccgac cgacgcaggt tcggccgcag ttgcatcagc
     1981 agttccccga cccctccatc cagttctggt tcgatgatct gcgccttcga ggcggaaatc
     2041 atgtatgaca cgaaaatcgc caccaggatg aacgagtcga gcgccgagag ggttcctttc
     2101 agtgggatga tgaggctata gatggtggcg agcacaagat gaaggagttc gatgtagcga
     2161 gatcggtcga ggacgacctg ccgatgtcgg gtcttaaacc agaatgtcat gacaacggag
     2221 gcccacccaa caccgatcaa caggcgattt gccccggtca tgttggcggt ggcgtaagcg
     2281 atgtaggtcg ggtccttgcc ggcagaccag gcgaagtaga tgtccaccgc atattccggg
     2341 agtacggcaa ccagcgcgag gaaggcgagc gcgagcgcct gagggatatc aacttgtgcc
     2401 acctcagccg cccaggagag gaggaacgcg gccccgaaga tggccaatcc agcgcccacc
     2461 acctcccatt gaggtggcag gtgaacatcg aagagtctga tgctcagcca ctgaaggggc
     2521 aaaaggatgg cggcgatcaa ccgcagcttt acgctcatcg cctgggtccc gcttgacttc
     2581 ccgcacccac cttccagagg ctcatgcgac ttcctgatcc tttactatgt ctcgctttcg
     2641 ccgctgccgc cgataatgat cgagcttgat ccatctgaag agcggctccc ggtacaggta
     2701 gagtagcaga cacgctacag acaggaagaa gatgagggca acgaggctgc cgtagtgagc
     2761 ggtccggacc tgcctcccct gcatggagag gagccaggtg cctgggagac ggcctacggc
     2821 ggtgagcagg aagaaggcgc cgaacgacag ggggctggca ccaaggatgt agcacaggac
     2881 gtccttcggg aaaccgggga tgagaaagag gaggaaggtg atcaacttgc cctgggtccg
     2941 cgtgagaaag aagaacatct cgtaggtctt gggtgtgacg aatcggcgag cgaaatgatg
     3001 tcccagccaa cgacccatgc tgaaggcgag gcaggagccg atggtcaggc cgatggtgga
     3061 atagaggaga ccggaaaacg tcccgaagag atagccaccg atgatgcccg tcacctcgcc
     3121 ggggatcggc gcaacaacga tctggagggc ctggatccct atgaaaacaa gaggcgccca
     3181 cgctccggac tgtttgatgt accgggctaa ggcctgttcg ttggcggagg gctcaatcca
     3241 ttcccagtac cttcccaggt cgagccactc gttaaacagg gcataggcgg atgccagacc
     3301 ggctacggca atgaggaccg tagcaatcat cacggttttc ttcattaacg atcgttgctc
     3361 gagcgttgta ggcaacacga aactagtcgc ggcgtccgac agggagcggt cgaacctggc
     3421 cggcctgagt caaaattcgc ctcgcctcat tgagctgatc agaccgcggg tatggctcag
     3481 tacggaagcc cgtcagtgat cgcgctgaga tgaaacgaca aggtgtagag cttgacttgg
     3541 agtgattggg gtgcagtatc gtcatatctc gcaactctct tacctgctaa gccacactac
     3601 tgtcaagaaa aatccttcca acctgcctga cggtagactg gtgccagcct ctttatctca
     3661 gaaccgatag actcgccatc ttgacgttac gggaccagcg gcttatccta ctgtacacga
     3721 c
//
LOCUS       DATMXI010000158         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_52541, whole genome shotgun sequence.
ACCESSION   DATMXI010000158 DATMXI010000000
VERSION     DATMXI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52541"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..893)
                     /locus_tag="VN444_02975"
     CDS             complement(33..893)
                     /locus_tag="VN444_02975"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018949746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR20809.1"
                     /translation="MATILIAGCGYIGTALGCQLAADGHTVWGLRRHPATLPPDIRPF
                     AADLTSPDTLQTLPPAIEWVVYSAAPDTHDDTAYRSVYVDGLNNLLHALTVQQAHPRR
                     VFLTSSTGVYGESSGAWVDETSPTQPKEFGGIRLLEGERLLLDGPFPATVLRLGGLYG
                     PGRASLIEQVRRGQIAWDNEPPVYFNRIHRDDAAGALRHLMMLQSPDPVYLGVDHEPA
                     TLAVLLDWLADALGVSPTRPGESSKTRTARHPANKRCRNAKLVASGYVFRYPTFREGY
                     AALLTEQTGP"
     gene            complement(1121..2029)
                     /locus_tag="VN444_02980"
     CDS             complement(1121..2029)
                     /locus_tag="VN444_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWR20810.1"
                     /translation="MAQLHILVTGSTGFVGSALVPFLAAGGHRITRLVRTTPEHGIAE
                     VQWDPEAGVMDIARLEGLDAVVHLAGENIAAGRWTAEKKVKIHDSRVGGTRLLCDSLA
                     GLKQPPKVLVCASAIGYYGDRGDELLTEESASGTGFLAGVCREWEAAASPAVQKGIRV
                     VHLRFGMVLSGAGGALARLLQPFKMGLGGVLGTGHQYISWIALDDLLGVIAHALTTET
                     LQGPVNAVTPNPVTNQEFTRTLGRIIGRFTPFAMPAVAARLTFGEIADELLLASQRAQ
                     PTRLLATGYRFRYPDLEDALRHSLGE"
     gene            complement(2151..2684)
                     /locus_tag="VN444_02985"
     CDS             complement(2151..2684)
                     /locus_tag="VN444_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20811.1"
                     /translation="MIKGMGERMLRAAKLDGSLYEEIKGDRRAPVQAAMVVILSAMAA
                     GVGAIVHDGLGGFFFVTMQAMVQWYVWAFFTYLVGTRLLQEPQTKMDCGTMIRIISFA
                     SAPGVVRLLGVIPYLGGFVSGLASVWMLGATVIAVRKTLNYTTTMRAVGACVIGWVAQ
                     YLFMGILENLRGPIPGA"
     gene            complement(3280..3675)
                     /locus_tag="VN444_02990"
     CDS             complement(3280..3675)
                     /locus_tag="VN444_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20812.1"
                     /translation="MKFLRKIFSTKPMAALEDNDESEEATADGPAPRKVKGVLILTRR
                     PLGDQHRLMEQIINHQRSQGYNIAWDLISRTSVTQGDFDDKAFLEATIRKQFVKLGSD
                     DIMDRTEVFPCQDTVGNSGVYCIIFNRRP"
BASE COUNT          824 a         1198 c         1088 g          831 t
ORIGIN      
        1 atgttgcggt atcggcgtga tcgtacacac catcatggac cagtctgctc tgtgagcagg
       61 gctgcatacc cctcgcggaa ggtcggatag cggaacacat agccggaggc aacgagcttg
      121 gcgttgcgac aacgcttgtt ggcgggatgc cgggccgtac gagtcttcga agattcacca
      181 ggccgagtcg gcgacacgcc caacgcgtca gccagccaat caaggagtac ggccagcgtg
      241 gccggttcgt gatcgactcc aaggtagacg gggtccgggc tctggagcat catcagatgg
      301 cgcagggccc ccgcggcatc atcccggtga atacgattaa aatagacggg cggctcatta
      361 tcccatgcga tctggcctcg acgcacctgc tcgatcagcg aggcgcgacc gggaccgtag
      421 agaccgccca agcgtaatac ggtagccggg aacggaccat ccagcagtag ccgctcgcct
      481 tcgagaagac gaattccacc aaactccttg ggttgggtag gggaggtctc atccacccat
      541 gcgccggaag actccccata tactcccgta ctcgatgtca ggaagacacg ccggggatgc
      601 gcctgctgca ccgtgagtgc gtgaagcagg ttgttgagcc cgtccacata gacggaccga
      661 taggccgtat cgtcatgggt atcgggggcg gccgagtaga ccacccactc tatcgccggc
      721 ggcagcgtct gaagtgtatc gggtgaagtc aggtcggctg caaatggccg aatgtccggc
      781 ggaagcgtag cgggatggcg ccgcagtccc cagacggtgt gcccatcagc cgcgagctga
      841 cagcccagcg ccgtgccgat atacccacag ccggctatca ggatcgtcgc cattttacaa
      901 tagcagcttt cagttatcag agttattacg gggagcacga actagattgc actcttgcac
      961 aacatctcaa gcaacctccc cctttgcgaa agggggattg agggggattt cctgatcgtt
     1021 caaatccccc cttacccccc tttgcgaaag gggggggcaa actattttat gctccgagta
     1081 atagccgacg gctttacccg ttcgcgctgg tagctgagcg ctactccccg agcgagtgcc
     1141 gaagcgcgtc ttcgagatcg gggtaacgaa agcgataccc tgtagccagg agccgggtgg
     1201 gctgggcgcg ctgactggcc agcagcaatt cgtcggctat ctcgccaaag gtgagacgtg
     1261 ctgcgacggc aggcattgca aacggggtga atcggccgat aatccgcccc aacgtccgcg
     1321 tgaattcctg attggtaacc ggattcggcg tcaccgcatt gaccggaccc tggagcgtct
     1381 cagtcgtgag cgcgtgcgca atcacaccca ggaggtcatc cagggcgatc cagcttatat
     1441 attggtgccc cgtccccaat acccctccaa gacccatttt gaacggctgg agcagcctcg
     1501 ccaacgctcc acccgctcca ctgagtacca ttccgaagcg gaggtgaacg acgcggatcc
     1561 ccttttggac ggccgggctg gccgctgcct cccattcgcg gcagaccccg gcgagaaagc
     1621 ccgttcccga ggcgctttcc tccgtgagga gttcatcgcc acgatcgccg tagtagccga
     1681 tagcagacgc acacaccagc accttaggcg gttgtttaag cccggcgagc gagtcgcaga
     1741 gcaacctggt gccgcccacc cggctgtcgt ggatctttac tttcttctcg gccgtccacc
     1801 ttccagccgc gatattttct cccgcgaggt ggaccaccgc atccagccct tcgaggcggg
     1861 cgatatccat cactcccgcc tctgggtccc actgaacctc tgcaatcccg tgctcaggag
     1921 tggtgcgaac caggcgcgta atacgatgtc cgccggccgc gagaaacgga accagcgccg
     1981 accccacgaa cccggtcgat cctgtgacga ggatgtgtaa ctgtgccatg agatacgctg
     2041 ccgcctttca gcgatgagcg atcagcttct gaggcccccc atcgccctgg ccgagttgtc
     2101 gggttacgct gcgctaaccc gacctacggc tgcatgctga tcgcttttgt tcatgcccct
     2161 ggaatcggac ctctcaggtt ctctaagatg cccataaaaa ggtactgcgc gacccatcca
     2221 atcacgcacg ctcccaccgc cctcatagtt gtcgtgtagt tgagggtctt tctcaccgca
     2281 atgaccgtgg ctcccagcat ccagaccgat gccagtccag agacgaagcc gcccaggtaa
     2341 gggatgacgc ccaacagcct cactactccc ggagcgctgg caaaactgat tatccgtatc
     2401 atcgtaccac agtccatttt ggtctgtggc tcctggagca atcgcgtgcc gaccaggtag
     2461 gtaaagaacg cccagacata ccactgcacc atcgcctgca tagtcacaaa aaagaaaccg
     2521 cctagcccgt cgtgaacgat ggcaccgact ccggcggcca tagccgatag gatcaccacc
     2581 atcgcggcct gtaccggggc ccgtcgatcg cccttaattt cctcataaag gcttccatcc
     2641 agcttggcag cccggagcat ccgctcgccc atccccttaa tcacctcttc tcctccacgc
     2701 cgctacattt acctcgcgtt gtccttggta gccgccactg ctgcctctgt ggcatctagt
     2761 tgagacctat acgcgaaaag cgctctgacg atgcaaacct atggtatcag ggtgagggtg
     2821 tcaactgttt tcggaatgga ggcaggcacc ccttggcgat ctgtagtgag gaacaaatgg
     2881 agtaagctct tggacctaca acccctcatg ggaggccggc cacttcaaca gggagaccgt
     2941 cccgctgctg ccatctactg tagcgtttca taactacggt gagaagggcc gttcaccttg
     3001 agcttgtcga agggcgagct ttttcaatgt gttccgttca tggttcgaca ggctcaccac
     3061 gaacggaatg ttgatggatg tatgaaacac gacactagaa gttttaggtg gtgaagcagt
     3121 ggtggagagg ataacagggc tgccgtgggc gccgccaagg ccaccctgaa gtccagtgcc
     3181 aacgatgtct tcagggtggc cgtctctacc ggtcgtctca acccttatgg gtgagcccgc
     3241 gccccgtgat atgagagcca tgcgtggtaa aacttcatac taaggacgac gattgaagat
     3301 gatgcaatac acgccagagt taccaaccgt atcctgacat ggaaacactt ctgtcctgtc
     3361 catgatgtca tcgctgccaa gtttcacaaa ctgttttcga atcgttgcct caagaaatgc
     3421 tttgtcatca aagtctcctt gagtcaccga tgtccttgag atcaagtccc aggcaatatt
     3481 gtacccttga gagcgctggt ggttaatgat ttgttccatc aatctgtgtt ggtcacccag
     3541 gggtcgacga gtaaggatca gcacaccctt cactttcctt ggcgcgggac cgtctgccgt
     3601 tgcctcttcg gactcgtcat tatcctccag cgctgccatt ggtttcgtgc tgaatatttt
     3661 acgcaggaac ttcattgtcg cttctccttt aagtgaaatg gtctcgaagt cactgatcgc
     3721 cgtgaccggt acttagcggt ctgcatctcc gatgttctcg gtttccagtt cagagtgtct
     3781 tttgtcgtca tgcgtctccg aggttacgtc ccgccggctt caagcaagat gactgtggac
     3841 atgaatcgct tcatgacgac gccttctgtt cgcttgcgtg aatctgtacg tcatgatcgc
     3901 agccagcgcc atgggcgcgg cgccgagcgc tatgaacacc g
//
LOCUS       DATMXI010000159         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_91231, whole genome shotgun sequence.
ACCESSION   DATMXI010000159 DATMXI010000000
VERSION     DATMXI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91231"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1313)
                     /locus_tag="VN444_02995"
     CDS             complement(99..1313)
                     /locus_tag="VN444_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HWR20813.1"
                     /translation="MKQLKGVLYRTVVMGVCLLSLSCGAEAAESIRVLLAADVSRLDI
                     RANGSLWVTDAKGRDQVLRAPVQVVADGAGFLLKGIRIQTEQLTLHGGEQGLVLTFPR
                     LTRKTNGVAMSTGDSSTEISLSGLVQLVRRGKGFLVINRVDLEEYVKGVVPAEVSSTW
                     HQEMLKAQAVAARTYALYQKMLSATREYDVAATVQDQVYRGKQGIDGGILRAVEETRG
                     LVVTYQNAPIYAAFSSTAAGLTEDAMNVWSKEYPYLKGVECPFDLASPYYQWKSSFKI
                     DTLEESLRQQGFSVGTIATIAPLSFSRGGRVATLRVVHSGGELVLRGEELRKAVGYTI
                     IPSTQFAIEFIGQDLVLSGFGAGHAVGMCQWGAKELAELGYSFSTILQYYYPGTELQS
                     LTLVRPPTPPSS"
     gene            complement(1310..1564)
                     /locus_tag="VN444_03000"
     CDS             complement(1310..1564)
                     /locus_tag="VN444_03000"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWR20814.1"
                     /translation="MTEWGILGKIFIGVGACVVLAGLLMLVADRIPGVGSMLGWFGKL
                     PGDISIRRDNFSFSFPIVTSLLLSVILSLVLYLITWIFRR"
     gene            complement(1564..2586)
                     /locus_tag="VN444_03005"
     CDS             complement(1564..2586)
                     /locus_tag="VN444_03005"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWR20815.1"
                     /translation="MLKKKPGYAVAIVGATGAVGKETLEILEERKFPLTVLRLFASKR
                     SAGEVLTCQDREWKVEELTPEVSFAGVDIAFISATDDISREYGRRLGAAGVVVIDDSG
                     VFRMEADVPLVVPEVNAHALRAMPRGIVSIPNCTTTPLVMALKPLQDAVGVKRVVVTT
                     FQSVSGTGAAAMDELMTQTKDLLAFRDAKAEVYPYQIAFNLLPHIGSFNNDGDCSEEV
                     KIAKETRKILEAPTLRVTATTVRVPVLRCHSEAINVELERPLKPNEARAALAAMPGVI
                     VYDDPSKKLYPMPLDATGKDDVYIGRIREDESITNGLNLWVVSDNLRKGAALNAVQIA
                     EHLAKG"
BASE COUNT          618 a          809 c          662 g          567 t
ORIGIN      
        1 cggtcgcaga acgagcagcc tggcctgatc gcgcgggaga caggggtaag cggcaatcag
       61 cgaagggtcg aatggaaaat cgaattcgga cagctgcatt acgaggacgg cggggtagga
      121 ggtcggacca gtgtcaggga ttgcagttcg gtgccgggat aatagtactg cagaatcgtg
      181 gaaaatgaat accctaattc agccagctcc ttcgctcccc actggcacat cccgacggcg
      241 tgacctgcgc caaagccgga aagcaccaga tcttggccga taaattcgat ggcaaactgt
      301 gtgctgggaa tgatggtgta accaactgct ttccgcaact cttctccgcg gagaaccaac
      361 tctccaccag agtggacgac acgcagcgtc gccacacgtc ccccgcggct gaaggacagg
      421 ggtgcgatcg ttgctatcgt ccccaccgaa aatccctgct gtcgcaaact ttcctcgagc
      481 gtatcgattt tgaacgagga cttccattga taatagggag aggcaagatc gaagggacat
      541 tcaacaccct tgagataggg gtactctttc gaccacacat tcatagcatc ttccgtcagg
      601 cctgcggctg tcgatgagaa ggctgcgtag atcggcgcat tctgataggt gacaaccagc
      661 ccacgggtct cttcgaccgc gcgcaggatc cccccatcaa tcccctgctt tccacgatag
      721 acttgatcct gcaccgtcgc cgccacatcg tactcccgcg tagcactcaa cattttctgg
      781 tagagcgcat aggtccgtgc ggcgaccgcc tgcgctttca acatttcctg gtgccaggtt
      841 gaactcacct ctgcgggaac cacccccttg acgtactctt caagatccac ccgattgatg
      901 actaaaaacc ccttacctct tcgcacgagc tggacaaggc cgctcagcga aatctccgtg
      961 ctggagtcgc ctgttgacat cgccactcca ttggtcttac gggtaagtcg tgggaaggtc
     1021 agcacgaggc cttgctctcc gccatgtaat gtcagttgtt ccgtctgtat ccggatgccc
     1081 tttagcagaa accccgctcc gtcggccaca acctggactg gggctcgcag aacctgatca
     1141 cgaccctttg catccgtcac ccacaaggaa ccattggctc gaatatctag cctggacaca
     1201 tcggccgcta acagaacacg gatcgattca gccgcctcgg ccccgcagga caggctgaga
     1261 agacacaccc ccatcacgac ggttcgatac agtacaccct tcagttgttt caccgtcgaa
     1321 agatccatgt tatgagatag agtaccagac tcaggatgac gctgaggagt aggctcgtga
     1381 cgatgggaaa cgaaaagctg aaattatctc gtcggatcga gatgtcgccg gggagcttgc
     1441 cgaaccatcc cagcatactc ccaactcctg gtatacggtc tgccaccaac atcaacaggc
     1501 cggcgagtac cacacaagcc ccgactccga tgaatatttt ccctagaatt ccccactcag
     1561 tcatcagcct ttggccaggt gttctgcgat ctgcaccgca ttcagcgcgg cccctttgcg
     1621 aagattgtct gataccaccc agaggttcag accattggta atcgattcgt cttctctaat
     1681 gcgaccgatg taaacatcat ccttcccggt cgcatccagc ggcatcggat agagtttctt
     1741 gcttggatca tcgtacacga tcacgccagg catcgccgcc aacgccgcgc gggcttcatt
     1801 cggcttgagc ggccgttcta attcaacatt gatcgcctca gagtggcaac gcagcaccgg
     1861 aacccgtacc gtcgtcgccg tcacacgcag cgtcggggct tcaaggatct tccttgtctc
     1921 cttggcgatt ttcacttcct cagaacaatc cccatcattg ttgaaggagc cgatgtgcgg
     1981 caagagattg aaagcgatct ggtaaggata tacttcggcc tttgcatcac gaaatgccaa
     2041 cagatctttc gtttgggtca tgagctcatc catcgctgcg gcaccggttc cagacacgga
     2101 ctgaaacgtc gtgacgacaa cacgtttcac ccccacagca tcctgcaacg gcttaagcgc
     2161 catgacgagc ggggttgtcg tacagttggg aatcgagacg attccgcgag gcattgctct
     2221 cagcgcatga gcattcacct ccggcaccac tagtggaaca tcggcctcca tgcgaaagac
     2281 cccgctatcg tcaatcacca cgacaccggc cgctccaagc cgacggccat attcgcggct
     2341 gatgtcgtcg gtcgcagaaa taaatgcaat gtcgacaccg gcaaacgaga cttcaggcgt
     2401 cagctcctcc actttccatt ccctgtcctg acaagtcaac acttcgccgg ccgaacgttt
     2461 cgaagcgaat agtcgaagga cggtcaatgg aaacttcctc tcttcaagaa tctcgagcgt
     2521 ttcttttccg accgccccag tcgcaccgac gattgcgacg gcatatcctg gtttcttttt
     2581 caacataagc ttcgacccta cctagagtac gatttcacga atgcgatgat taaaggtatc
     2641 ggccacgagc acatgc
//
LOCUS       DATMXI010000160         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_199939, whole genome shotgun sequence.
ACCESSION   DATMXI010000160 DATMXI010000000
VERSION     DATMXI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199939"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VN444_03010"
     CDS             <1..347
                     /locus_tag="VN444_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWR20816.1"
                     /translation="EQVRLTAGLQVVFSRRGERADQVIVRFVEKAPSGAVVVTSDRAL
                     ADDVARTGALVLSAEEFHERLDLALREGDGGEFQKDDDSVPESPGPRILKGPARRPSR
                     QAKRRITTLKRL"
     gene            complement(593..865)
                     /locus_tag="VN444_03015"
     CDS             complement(593..865)
                     /locus_tag="VN444_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWR20817.1"
                     /translation="MTKAELVDKIAKDACITKKAAEQALCSVTAGVRDSLKKGKKVTL
                     VGFGTFSVAKRAARNGRNPQTGAVIKIKAAKIPRFRAGKALKDAVK"
     gene            1307..1780
                     /gene="smpB"
                     /locus_tag="VN444_03020"
     CDS             1307..1780
                     /gene="smpB"
                     /locus_tag="VN444_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWR20818.1"
                     /translation="MASTQERRILCSNRRARYEYQIEEVIEAGIALTGTEVKSLRDGK
                     ADLKDSYAAIEGQEAYLFNCHISPYTAGNRFNPDPNRKRKLLLNRMEIARFTGKVQEK
                     GLTLIPLSFYLVRRKIKLELALARGKKLYDKRETLKQRAMTKEMDQLARGRADRG"
     gene            1869..2219
                     /gene="ssrA"
                     /locus_tag="VN444_03025"
     tmRNA           1869..2219
                     /gene="ssrA"
                     /locus_tag="VN444_03025"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          538 a          688 c          695 g          506 t
ORIGIN      
        1 gcgagcaggt gagactcacc gctggcctgc aggtggtctt ctcgcgccgc ggggagcggg
       61 ccgatcaggt catcgtgcgg tttgttgaga aagccccctc aggggcggtc gtggttacct
      121 cagatcgggc tttggcagac gacgtggcgc ggaccggcgc cttggtcctc tcggccgagg
      181 agtttcacga gcgcctggat ctcgcgcttc gagaagggga tgggggcgag tttcaaaaag
      241 atgacgattc ggtccccgaa tccccagggc ctcgaatact aaagggtccc gcaagacgcc
      301 catcgaggca agccaagcgc cggatcacca ctctcaagcg cttgtgagag tttcaggggt
      361 ttaggctgaa gaccgaagac tgaagcatat caatcattac gcaaggcacg aatcgagtcg
      421 ttcaagacct tctcggtttc tacccctaaa agccttcagc cttcagtcta tctacctaag
      481 tagtcccgaa ccggtcacaa atacgaagag gagagacacg gtctctcctc ttcgtcgtat
      541 actatgacgt ctgatgcgat agtcgatgga ttagggcacc agcaaactcc acttacttga
      601 cggcgtcctt caatgccttt cccgccctga accgcgggat cttcgctgcc ttgatcttga
      661 taacagcacc ggtctgagga ttgcgcccgt tccgggccgc tcgcttcgcc accgagaacg
      721 tcccaaagcc gaccagggtg accttcttcc ctttcttgag agagtcccgc acccctgctg
      781 tgacactgca cagggcttgt tctgccgcct ttttcgtgat gcacgcatcc ttggcaatct
      841 tatctacgag ttccgccttg gtcatgcccc cccctttctc aatggattca ctaataataa
      901 gtagatcacc atcggtcctt gccgacttgc ctagaaagct cttaccaaac tcccccatga
      961 tctgtcaagg gaaaaaagac cttagtaatt aaacgtagca cagaacagtt caggcgtatg
     1021 gtctccagcc ctcgagttgg tgaagagctg ctgtcccaat gcccgtatcc aacataggca
     1081 ggggtggacg tcactcctca ggtaaatagg ctgaaggctg aaggctttta gtggtaaagc
     1141 gatgaagacg cacagtgtcg aagcgtgtgg gcttcttgcc caacgaggag tcctctttga
     1201 gagaaccaca ggtataggcc gccttaagcc ttcagcctaa acgcctaagt agtggacctg
     1261 gaattccact tgccgcgtag tgttgggcta cgctatagtt cataaaatgg catcgacgca
     1321 ggaaagaaga atcctctgct ccaaccggcg ggcgaggtat gagtaccaga tcgaggaggt
     1381 catcgaggcc gggatagctc ttaccggaac cgaggtgaag tcgctgcggg atggcaaggc
     1441 cgacctgaaa gatagttatg ccgccattga ggggcaggag gcatatctat tcaactgcca
     1501 catcagtcca tacaccgcgg gaaatcgatt taacccggac ccgaatcgta agcggaaact
     1561 tctcctgaac aggatggaga tcgccaggtt tacggggaag gtccaggaaa agggcctcac
     1621 cttaattcct ctgagtttct atctggttcg acgaaagatc aagctggagc tggcgctggc
     1681 ccgtgggaaa aaactgtacg ataagcggga aaccctgaag cagcgcgcga tgacgaaaga
     1741 gatggaccaa ctcgctcgcg gccgtgcgga ccgggggtag ctgttgactt tttcagccag
     1801 gcggcataac ataaaggtag tctgttacct ccaggcccaa ccgggtgtgg catgcgcgtt
     1861 attattgcgg gggcgctccg gtttcgacgg ggataaggga gtaagggctg cgtgccgagg
     1921 ccccatcgcc tcgttaaaag gtgggaaaac aagcaactgc caaccacgaa ttggcactgg
     1981 ccgcctaaga gtggccacgt tcaccccacc tcgctcgtcg ggtgggatct gggcgccgac
     2041 tagacgggct ggcttgcctc tcgtgctctg gggaggcggg taagatccac atgagctagc
     2101 cgctccggga ccctgcctgt gggggccaag agaggcgaat attcatacac aggctacgca
     2161 cgtagatgtt ctttccgaca tgttctcgga cgcgggttcg actcccgccg cctccaccaa
     2221 cgcaatgcag ttgaaccgcc ctccgggccc cacaactcgg agggcggttc cataagcgcc
     2281 aggacaaggt ccttaactcc acggcaagct gtgggggatc gtcttctgtt ctggcccgtc
     2341 attccgtgct tgacacggaa tccagtcggg ctctctggat accggcgcct gccccggacc
     2401 ccgatccggg gtccgccggt atgacga
//
LOCUS       DATMXI010000161         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_27877, whole genome shotgun sequence.
ACCESSION   DATMXI010000161 DATMXI010000000
VERSION     DATMXI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27877"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1312)
                     /locus_tag="VN444_03030"
     CDS             complement(92..1312)
                     /locus_tag="VN444_03030"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HWR20819.1"
                     /translation="MMKLPIFLDNHSTTPVDPRVLETMLPYFSEKFGNAASRNHAFGW
                     AAEEAVENARKQIAKLIKADPKEIVFTSGATESDNLAIKGVLDMYKEKGDHIITSSTE
                     HRAVLDTVKSLEGRGKASATYLPVDKFGMVNPEDVRKAITEKTILISVMLANNEIGTI
                     NPVKEIGKIAKEKGILFHCDATQGVGKIPVDVQDMGIDLMSFAAHKMYGPKGVGALYV
                     RKKNPRVRIVAQMDGGGHERGMRSGTLPVPLIVGFGKACEICEQEMAKEAARLTAMRN
                     RLEASIMAALEESYLNGHPTNRLPGNLNISFAYVEGESLLMGMKDIALSSGSACTSST
                     LEPSYVLRALGVGTELAHSSIRFGLGRFNTDEEIDYTIKKVIEVVTKLREMSPLYEMA
                     KEGVDLKSVRWAAH"
     gene            complement(1309..1779)
                     /locus_tag="VN444_03035"
     CDS             complement(1309..1779)
                     /locus_tag="VN444_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWR20820.1"
                     /translation="MLKISKKADYALMALQHIATVQFGDVMPGRVVNTKEIAEEYNIP
                     LELLAKVLQTLAKNGLIESHNGPKGGYVLARRAHQITIAQILESIEGPLGITDCSHEK
                     DGELCMQRENCHIRTPLLKVQDSIAQLLNSMTLQDMMGGTPLITIQSPTAQGVE"
     gene            1908..2324
                     /locus_tag="VN444_03040"
     CDS             1908..2324
                     /locus_tag="VN444_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWR20821.1"
                     /translation="MGGTNPYIEKAEYELPRTAYTVTFIAPDGMVTKVEVDPAKIPYG
                     PTGLPGSLLDVAMGNGVALEHVCGGVCACSTCHVIVKQGLESCNEGTDDEFDQLEEAP
                     MTTLQSRLGCQCVPNGTKDILVEIPAVNKNLVKEGH"
     gene            complement(2331..>3006)
                     /locus_tag="VN444_03045"
     CDS             complement(2331..>3006)
                     /locus_tag="VN444_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR20822.1"
                     /translation="LSITDLTPNVRQLVVLPQTQNIAFRPGQWVSLKLPVGARPPLNR
                     AYSMAAPGTTSGELTLVFDRVVGGLGSGFLYGLKPGDTVQLSGPYGNFTVPEPFDREL
                     LLIARYTGLVPIRCILKQLYAQRQTGSVLLIAVAPAEDEFLFHQELLTLAVTNPGLRY
                     LPLVAAGGEQQGVDLTLSMLRPLIEDAPKVIPMLCGTKAFVRPLRAYFVQTGYDRKEV
                     KVETYD"
BASE COUNT          668 a          812 c          825 g          701 t
ORIGIN      
        1 ctgtaagcca taaatacctc ctgtatctcg ttgatcgtga agcgtatctc gtttgtttcg
       61 cttcacgcgg tacgtttcac gtttcacgcg ttcagtgcgc ggcccaccgg actgatttca
      121 gatcgacgcc ttcttttgcc atctcgtaca gcggggacat ctcgcgaagc ttggtcacga
      181 cctcaataac cttcttgatc gtatagtcaa tctcctcgtc cgtattgaaa cggccaagac
      241 cgaagcggat cgaggaatgt gccaactccg tcccgacacc taacgcacgg agcacgtatg
      301 aaggttccaa ggtggacgag gtacaggcag aaccggacga gagggcgata tccttcatac
      361 ccatcagcaa ggattcacct tccacatagg caaaggagat attgaggttg ccggggagcc
      421 ggttggtcgg atggccgttc agatagcttt cttcgagtgc cgccatgata ctggcctcca
      481 gccgattgcg catcgcagtc aaccgggcag cctccttggc catctcctgc tcgcaaattt
      541 cacaggcctt accgaatccc acgattaagg gcaccggaag tgtcccggaa cgcatgccgc
      601 gctcatgccc gccgccgtcc atttgcgcga cgatgcgaac tcgaggattt ttctttctca
      661 cgtagagcgc cccgaccccc ttgggcccat acatcttgtg ggcggcgaac gacatcagat
      721 cgatgcccat atcctggaca tcgaccggga tcttgccgac accctgcgtc gcatcgcagt
      781 gaaaaaggat gcccttttcc ttggcaattt taccgatctc cttgacggga ttgatcgtgc
      841 cgatttcgtt gtttgccagc atgacggaaa tgaggatggt cttctctgta atggctttcc
      901 gcacatcctc gggattcacc atcccgaatt tatcgacggg gagataggtc gctgatgctt
      961 tccctctgcc ctctaacgat ttgactgtat cgagcactgc ccggtgttcg gtggacgatg
     1021 taataatgtg gtcgcccttc tccttgtaca tgtccagcac gcccttgatc gcgagattat
     1081 ccgattcggt ggcgccgctc gtaaagacga tctcttttgg atcggccttg atcagtttcg
     1141 cgatctgttt ccgtgcgttt tcgacggcct cctctgcagc ccacccgaaa gcatggttac
     1201 ggctggccgc gttcccgaac ttctcggaga agtaagggag catggtttcc aatacgcgcg
     1261 gatctaccgg tgtcgtggaa tggttgtcca aaaaaatcgg cagtttcatc attcaactcc
     1321 ttgtgccgtg ggagactgga ttgtaatgag gggtgtgcca cccatcatgt cttgcagggt
     1381 catgctattg agtaattgag cgatactgtc ctggaccttc aagagcggtg tgcggatatg
     1441 gcaattttcc cgctgcatac agagttcccc atccttctca tgagaacaat cggtgatacc
     1501 taaggggccc tcgatgcttt cgagaatctg ggcgattgta atctggtgtg cacgtcgggc
     1561 cagcacgtag ccgcccttgg gaccattatg actttcaatc agtccgttct tggcgagggt
     1621 ttggagcact ttggccagca gttcgagggg gatgttgtac tcctccgcga tctccttcgt
     1681 attcacgaca cgtccaggca tcacgtcgcc gaactgaaca gtcgcgatgt gctgaagtgc
     1741 catgagggca tagtctgctt ttttcgaaat ctttaacatt gctattgccg atcataacag
     1801 tcggagacta taatgatctc cgatcaataa ggtcggacta agaatagcat gcacatttct
     1861 aagctgtcaa cagcaagacc tcagtaggct gagaaaggac cagatacatg ggcggaacca
     1921 atccttatat tgaaaaagct gaatatgagc ttcctcggac agcctatacg gtcaccttca
     1981 ttgcccccga cggcatggtc acgaaggtcg aagtcgaccc ggcaaaaatc ccctatggcc
     2041 cgacggggct ccctgggagc cttctggatg tggcgatggg aaatggagta gccttagaac
     2101 atgtctgcgg cggggtttgc gcctgttcaa cctgccacgt catcgtgaag cagggccttg
     2161 agagttgtaa cgaggggacg gatgatgaat tcgatcagtt ggaagaagcg ccgatgacga
     2221 cgctgcaatc acggctcggt tgccaatgtg tgccaaacgg gaccaaggat attcttgtcg
     2281 agattccggc ggtcaataaa aacctcgtta aggaaggcca ttgactttct tcagtcatac
     2341 gtttcgacct tcacttcttt tcggtcgtac ccagtctgga cgaaataggc gcgcaacgga
     2401 cgcacaaaag ccttcgtccc gcacagcatg ggaatcactt tcggcgcgtc ttcgatcaag
     2461 gggcggagca tcgacagcgt gagatcgacc ccttgctgtt ccccgccggc agcgaccaga
     2521 ggcaaatagc ggagcccggg attggtcaca gccagcgtga gcaattcctg atgaaaaaga
     2581 aattcgtctt ctgcgggggc cactgcgatc aacaagacgg acccggtctg tcgctgggca
     2641 tagagctgtt ttaatatgca gcgtatgggg acgaggccgg tgtagcgcgc gatgagcaac
     2701 agctcccggt cgaacggctc tggaacggtg aaatttccat agggcccgga tagctgtact
     2761 gtgtcgccgg gtttgagccc atagagaaag ccggatccca aacctccgac cacacgatca
     2821 aacaccaggg tcagttcgcc ggaagttgta ccgggggcag ccatcgaata ggcacgattg
     2881 aggggaggcc tcgctccgac aggcagcttc aatgacaccc actgaccagg ccggaaggcg
     2941 atgttctgtg tctgaggaag cacaacaagc tgccggacat tgggagtgag atcggtgatc
     3001 gagagg
//
LOCUS       DATMXI010000162         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_223019, whole genome shotgun sequence.
ACCESSION   DATMXI010000162 DATMXI010000000
VERSION     DATMXI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223019"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VN444_03050"
     CDS             <1..293
                     /locus_tag="VN444_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWR20823.1"
                     /translation="ELRRWNDNDLRGQTKIRPGQALQVVAQAVKGNREARRSAPASTA
                     PALHHRVKRGDTLWAIARVHDVTPEELRRWNDLGRQAKLRTGQDLMIRLSES"
     gene            395..646
                     /locus_tag="VN444_03055"
     CDS             395..646
                     /locus_tag="VN444_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20824.1"
                     /translation="MRGPVVPPGGPDRHFRFKAGVTFIAASFLVYPAYVAIPFLPLST
                     TLKLNITLLASLLSWGAFSIGSYLSGREGYEWLKRRLRR"
     gene            1001..>2100
                     /locus_tag="VN444_03060"
     CDS             1001..>2100
                     /locus_tag="VN444_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWR20825.1"
                     /translation="MNEVSISNLLVRGKRVGLTLVAALTAAVTAVSPVSAAQSPQVPL
                     DASTIPQFAQSLPLLSIGGDGTSGIATFGGIVPVEIHICEFNAKVLPPATPGVTANTR
                     SWGYVYGTACPDPSDATYTRDSFIGPVFVAIRDMGTQLKVVNDLGTAATTGVLAYKYS
                     VDQTVHWADPLNGEAGDCHHAAQMGEIPTPGSECAENYAGPIPAAVHLHGGLVPAELD
                     GGPDAWVTSTGIKGHGYYTSATAPANGFVYNYPNRQEASALIYHDHILGALRLNLYAG
                     IAGAYVIEDPAIIPTDATGVWVSNAIASPPINSCNPKSKKCLPSNLPGSAEIIPVVIQ
                     DRIFDTNGQLFWPADTAGGVQSSPNPQHPYWAP"
BASE COUNT          419 a          644 c          602 g          435 t
ORIGIN      
        1 aagaactccg ccgatggaac gataacgatc tgaggggcca gacgaaaatc aggccggggc
       61 aagcattaca ggtagtcgct caggccgtca aggggaaccg tgaggctcgc cggagcgctc
      121 ctgcttccac agcccctgcc ctgcaccacc gggtgaaaag aggcgatacc ctgtgggcga
      181 tcgccagagt ccacgatgtc acgccggaag aactccgccg atggaatgac cttgggcgtc
      241 aggccaagct ccggactggt caggatctga tgatccgcct cagcgagagc taacgaggca
      301 tcttcgacct cttaccagac tttttccagc ccttttggcg tcatagcgtg aggcgcagtg
      361 cgccctgaga gctggagcag gttctttcaa cgccatgcgg ggaccagtcg tgccaccagg
      421 cggtcctgac cgacattttc ggttcaaggc aggcgttacc ttcattgctg caagcttctt
      481 agtctatcca gcctatgttg cgatcccgtt tcttccgctt tccactacgc tgaagctcaa
      541 catcaccctc ctcgcgtcgc ttctgagctg gggggccttt tccatcggct cctacctgag
      601 tgggcgagaa gggtatgagt ggctcaagcg acgcctgcgt cgctgaagcc gttccgggca
      661 aatcgatcgc ctccttggtt cgtcgcaaat gaggctttgg acgccctgcg cacacctctt
      721 tttcccttgt ccctactaca caggataaga ctatatacgc caaaaagcgt attgacagac
      781 accgcgactg agcatatgct cccccactgt agaatgtagt gtttcagtag tggttctgct
      841 gctccgctct gccttgccgt cgaactacct acggcaagct cgtcgaagct gctcgccacg
      901 aacggagcgt aaaagaattg gtgtaacact acgataagct gcggtgttcc atggtggcgg
      961 atcttccgcc gacacttatc accatgtggg aggcgtacat atgaatgagg tgagtatcag
     1021 caacttactg gtaaggggta agagggtggg gcttacgctg gtcgccgcgt tgacggctgc
     1081 ggtgacggcg gtttccccgg tatcggctgc ccaaagcccg caggttccgc tagatgccag
     1141 taccattccg cagttcgcgc agtcgctgcc gctgctctct atcggcggcg acggtacttc
     1201 tggcattgcc accttcggcg gtatcgtgcc ggtcgagatc catatctgcg agttcaatgc
     1261 aaaggtgtta ccacccgcca ccccgggtgt cacggccaat acacgttcgt ggggctatgt
     1321 atacggtacg gcctgtccgg atcccagcga tgcgacctac acgcgagaca gctttatcgg
     1381 cccggtattc gtagctattc gcgatatggg gacgcaactg aaggtcgtca acgatctcgg
     1441 aaccgccgcg acgactgggg tgctggccta caagtattct gtggatcaga cggtacattg
     1501 ggccgatccg ctgaacggtg aggccggcga ttgccatcat gccgcacaga tgggagagat
     1561 cccgacgccc ggaagtgagt gcgccgaaaa ctatgccggc cctatccccg cggcggtcca
     1621 cctgcacggc ggcctcgtcc cggcagagct ggacggcgga ccggatgcct gggtcacgag
     1681 cactggcata aaaggccatg gctactatac ctccgccacg gctccagcca acggcttcgt
     1741 ctataactat cccaaccggc aggaggcctc ggcgctgatc tatcatgatc acatcctggg
     1801 tgccctgcgt ctgaacctgt acgcggggat cgcgggcgct tacgtgattg aagaccccgc
     1861 gattattccg accgacgcaa ccggggtgtg ggtgagtaac gcaattgcca gtcctcccat
     1921 caactcctgt aatccgaagt cgaagaaatg tctgccttcc aacttgcccg gctccgccga
     1981 gatcatcccg gtggtcatcc aggaccggat attcgacacc aacgggcagc ttttctggcc
     2041 ggctgacact gccggcggcg tccagtcgag cccgaacccc caacatccct actgggcgcc
//
LOCUS       DATMXI010000163         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_222983, whole genome shotgun sequence.
ACCESSION   DATMXI010000163 DATMXI010000000
VERSION     DATMXI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222983"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..908
                     /gene="pstB"
                     /locus_tag="VN444_03065"
     CDS             144..908
                     /gene="pstB"
                     /locus_tag="VN444_03065"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWR20826.1"
                     /translation="MMITEPMIEISKLTLKYGEKLALQDISLDIPKHQVTAFIGPSGC
                     GKTTLLRCLNRMNDLIDGVTVSGTIRIGGLDIHDPAMEITELRKRVGMVFQKSNPFPK
                     TIYDNVAYGPRILGVRGQSTLNEIVERSLRAAALWNEVQDRLRSSALGLSGGQQQRLC
                     IARAIAVEPEVLLMDEPCSALDPIATGKIEELMADLKQQYTIVIVTHNMQQAARVSDY
                     TAFLYLGQLVEYDLTKQLFVKPSKKQTEDYITGRFG"
     gene            926..2275
                     /gene="phoU"
                     /locus_tag="VN444_03070"
     CDS             926..2275
                     /gene="phoU"
                     /locus_tag="VN444_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWR20827.1"
                     /translation="MQRHFDEQLQELREHLLAMGSLAETMIVKSVKALMDRSEALVQE
                     VFTHEEEMDQRCIETDDRCFTLLALQQPMARDLRFIAAAIKINSDIERIGDLAVNIAQ
                     ATMSLIRQPILKPLIDIPRIAQLCQEMVKKSLDAFVAQDPELARIVIESDDSVDLLRD
                     QIFRELLSYMISDPTTIPRALDLVLISRYLERIADHATNIAEDVVYIVRGQDIRERGD
                     KEIRKGLRRPAGISPEIPGVDREAALAAHRLMPEEREFLSLIESAAHNLLDAARSLQA
                     MFEDYTDPAEKWRTIREAEHQGDAITHDIMKKLNQTFIPFIDRQNLHALTSALDDVVD
                     FIEAAASRMVLYKIDQPTSESREMVALIVASAEQVAKAVSQLPRFDTVQEICVEINRL
                     ENTADDLYRRAIARLFEGDLPVLEVTKWKEIYESLEGVTDRCEDVANVVETIALKYA"
     gene            complement(2321..>2799)
                     /locus_tag="VN444_03075"
     CDS             complement(2321..>2799)
                     /locus_tag="VN444_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20828.1"
                     /translation="AKPSSLVDPGQREVGPFLIRGAVTEFNEIAQASGESSGGSLGAL
                     GAALGIAGAIAGNTAAGIAGGAVALANPTYENTVARRTGSVAMDLRVVKPTDGRLIGS
                     VVANGSFTSESAASGFSLFGFGKASNAFAASALGQANRAAMNSAATQIFKQLQNIR"
BASE COUNT          634 a          816 c          832 g          517 t
ORIGIN      
        1 ggataccggc ttccgccggt atgacgaact cgcggcaagc cgcggggtat caaccctcaa
       61 cggaatgaat gtaaagaagc ggcgggcggc gcctggtggg aatggtgcag ggccatgaga
      121 tttcgaatgg actgtaataa ccgatgatga taacggagcc gatgatcgaa attagtaagt
      181 tgaccctcaa atacggcgag aaactcgccc tccaggatat cagcctcgat atccccaagc
      241 accaggtcac ggccttcatc gggccatctg ggtgcgggaa gacgacgctc ctgcgatgcc
      301 tgaaccgcat gaacgacctc atcgacgggg taaccgtcag tgggactatc cggatcggcg
      361 gactcgacat ccatgacccg gccatggaaa tcacggaact ccgcaagcgg gtaggcatgg
      421 tgttccagaa atcgaatccc tttccgaaga cgatctatga caacgtcgcc tatgggccaa
      481 ggatcctcgg tgtacgcgga cagtcgacgc tgaacgagat cgtggaaagg agtctgcggg
      541 cagccgccct ctggaacgag gtccaggatc gcctgcgcag tagcgcgctc ggcctgtcag
      601 ggggacaaca gcagcgactg tgcatcgcca gggccattgc tgtcgaaccg gaggttctct
      661 tgatggacga accgtgctcc gcgctggatc cgattgcaac gggaaagatc gaagagctga
      721 tggccgacct gaagcaacag tacacgatcg tgatcgtcac ccacaatatg cagcaggcgg
      781 ccagagtctc ggattacacg gctttccttt accttggcca attagtggag tacgatctga
      841 ccaagcaact ttttgtgaaa ccctccaaga aacagactga ggactacatc acgggccgat
      901 tcggctagga cacgagggcg gagccatgca gcgtcacttt gatgagcaat tgcaggaact
      961 gagagaacac ctgttggcga tggggagtct ggccgagacg atgatcgtga agagcgtcaa
     1021 ggcgttgatg gatcggagtg aggctctggt gcaggaggtg tttacccacg aggaggagat
     1081 ggatcagcgg tgcatcgaaa ccgatgatcg atgcttcacc cttctggccc ttcagcagcc
     1141 aatggctaga gacctccgtt tcatcgcggc agccatcaag atcaacagcg acatcgagcg
     1201 aatcggtgac ctggcggtca acatcgcgca ggcgaccatg tcgctcatca gacagccaat
     1261 cctgaagccc cttatcgata tcccacggat agcccagctc tgccaggaga tggtgaagaa
     1321 aagcctggat gcttttgtgg cacaagaccc ggagctggca aggatcgtca tcgaatcgga
     1381 cgattccgtc gatctcctgc gggaccagat cttcagagag cttctcagct atatgatcag
     1441 cgatcccacc acgattccca gggccttaga cctggtgctc atctcgcgct acctcgagcg
     1501 gatcgccgat catgcgacta atattgcaga ggatgtcgtc tatatcgttc ggggacagga
     1561 tatccgggag cgtggcgaca aggagatacg aaagggcttg agacgcccgg ccggtatctc
     1621 accagaaatt ccaggagttg atcgagaggc agcccttgcg gcccacaggc tgatgccgga
     1681 ggagcgtgag tttctcagcc tgatcgaatc cgcagcgcac aatctcctcg atgccgccag
     1741 gtccctgcag gccatgttcg aggattacac cgatcccgct gagaagtgga gaacaatcag
     1801 ggaggctgag caccaagggg acgctatcac ccacgacatc atgaagaagc taaaccagac
     1861 cttcatcccg ttcatcgacc ggcagaacct ccatgccctg acgtcagccc tcgatgacgt
     1921 cgtggacttc atcgaggcgg ccgcctccag gatggtgctc tataagatcg atcagccgac
     1981 ctccgaatct cgcgagatgg tcgccctcat tgtggcatcg gctgagcagg tcgcgaaggc
     2041 cgtcagccaa ctgccaaggt tcgacaccgt gcaggagatc tgcgtggaga tcaaccggtt
     2101 ggagaacacc gcagacgacc tctatcgccg cgccattgcc cgcctgtttg agggcgatct
     2161 tccggtcctc gaggtgacca agtggaagga gatctacgaa tcgctggaag gtgtgaccga
     2221 ccgctgtgag gatgtggcga acgtagtcga aacgatcgcc ctcaagtatg cgtgagtctg
     2281 agccactcgt ctaacctgga agtcggaaca agcccctcgt tcaacgtatg ttttgcagtt
     2341 gcttaaagat ctgggtagcc gccgaattca tcgcggcacg attggcctgt ccgagcgcac
     2401 tggccgcgaa ggcgttgctc gccttaccga atccgaacag cgagaagccg ctcgccgccg
     2461 actcggacgt gaagctgccg tttgccacca cgctgccgat aagtcgtccg tccgtcggct
     2521 tcaccaccct gagatccatc gccaccgaac cggtccgtcg cgccaccgta ttctcatagg
     2581 tcgggttggc cagcgcgacc gccccgccgg cgatgccagc agcagtattg ccggcgatgg
     2641 ccccggcgat accgagagcg gccccgagcg cgccgagcga gccgccgctg ctctcaccag
     2701 aggcttgagc gatctcgttg aactcggtca ctgcgccgcg gatgagaaag ggtccgacct
     2761 cccgctggcc ggggtccacg agacttgacg gcttcgcct
//
LOCUS       DATMXI010000164         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_267189, whole genome shotgun sequence.
ACCESSION   DATMXI010000164 DATMXI010000000
VERSION     DATMXI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267189"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1272)
                     /locus_tag="VN444_03080"
     CDS             complement(298..1272)
                     /locus_tag="VN444_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWR20829.1"
                     /translation="MNLDERNDDRPIRSGELPIVADPPAAPSDRPVDDEFEETPFSKW
                     TLPISLFIATIFTTLWAGAYQVYNGPIRGPVNLLLEEPTILWRGIPFAATLLAILVTH
                     ECGHYVLSRIHRVPASLPLFIPGPPYFIGTFGAIIRLHGPIMNRRALFDVGVSGPLAG
                     FIAAVMALVVGLNLSTVVEHPSTHGMQLGEPLLLQFIAWLIMGSIPPQADIILHPIGF
                     AAWFGLFVTSLNLLPIGQLDGGHVAYALWGRHQRTMAFVMVPILIVLGYVGWPGWWLW
                     AFMAGLWGFGHPPVHDLHVPLGRGRIAVGWIALAVFVVTFAPVPFSFR"
     gene            complement(1286..2125)
                     /locus_tag="VN444_03085"
     CDS             complement(1286..2125)
                     /locus_tag="VN444_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR20830.1"
                     /translation="MPLEDDFSDILKKARTGQGLSVGDIARATGLSTEAITTLERGDQ
                     PHDRAEVQILAKALGLRSAPLEQIVIDKWEPIPQRMPVWIETVHGSINGYGVQGYILH
                     DGGEALLIDTAYNAPAMIAWLESHCVRLTGICLTHGHADHAEGIQELLARWQVPVYLG
                     ADDVSLLHWKPSKDRLTAPNDDRTIPVGRLSVRCLTTPGHTPGGICYRIDAGSQPVCF
                     VGDTVFAGSIGRSNPHTLYRTHLDSVQQRVLKLPDDCILLPGHGPATTVREELDHNPF
                     AQD"
     gene            complement(2231..2524)
                     /locus_tag="VN444_03090"
     CDS             complement(2231..2524)
                     /locus_tag="VN444_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWR20831.1"
                     /translation="MVTAVMLLNWTDHGIKNVKESPKRLDGVKKLAKVMGGEVKSVYM
                     TLGVYDLVLIVDMPNNDKLASFGLKLGSLGNVRSTTLKAFPEDDYRRIISGLS"
     gene            complement(2751..>2962)
                     /locus_tag="VN444_03095"
     CDS             complement(2751..>2962)
                     /locus_tag="VN444_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWR20832.1"
                     /translation="LEYDGLPKDFLQQLRARVIALTKEDILAAAKKHFHPERLTILAV
                     GSGEALPKLLSGFGEVKEINLAPES"
BASE COUNT          657 a          847 c          871 g          587 t
ORIGIN      
        1 gataatcccg catttcacac actcggtcgc tccgtcaggc tgctcagcct gacacttggg
       61 acaggacagg gtcggggtcg tagcggatgc catagcgaca tggtagggct gagtacgcag
      121 ttgaacaaga gaatctctag attaatccaa tctgctgaat aagttcgatt gcgggatgct
      181 caaaaaggcc gtccagcaag gccgcagccg atgaaagcac ctgaggcgta tcctcagggc
      241 tacgttgagg atgctttcga ggggagaacg acggtggcgg actttttcag catcccgtta
      301 gcgaaaggag aagggaacag gagcaaaagt aacgacaaac accgcgagtg cgatccatcc
      361 gacagcgatg cgtcctcggc ccagcggcac gtggagatcg tgtaccggcg gatgtccaaa
      421 accccacaag cctgccatga aggcccagag ccaccaaccg ggccatccga cgtatcccag
      481 cacgatgaga attggaacca tgacaaaggc catcgtgcgt tggtgtctac cccacagggc
      541 ataggcgaca tgtcctccgt cgagttggcc gatgggaagg agattcagtg acgtgacaaa
      601 caggccaaac catgcggcga atccgatagg atgcaagatg atatcggcct gcggaggaat
      661 ggatcccata atcaaccagg cgatgaattg caagagcaac ggttcgccca actgcattcc
      721 atgagtagac gggtgttcga caaccgttga taggttcagg ccgacaacca atgccatcac
      781 tgcggcgatg aaaccagcca acggaccgga tacgccgaca tcgaacagtg cgcgtcggtt
      841 catgatcggc ccgtgcaggc ggatgatcgc cccgaaggtg ccgatgaagt aggggggacc
      901 aggaatgaac agcggcagcg acgccggcac cctgtgaata cgtgaaagca catagtgccc
      961 gcactcatgg gtgaccagaa tcgccagcag cgtggcggca aagggaatgc cgcgccagag
     1021 tattgtgggt tcttcgagca gaagatttac cggcccacgg atcggcccgt tgtagacttg
     1081 ataggccccg gcccatagtg tggtaaagat cgtcgcgata aagaggctga tgggcagggt
     1141 ccatttcgag aagggggtct cctcgaattc gtcgtccaca gggcgatctg agggggccgc
     1201 aggcgggtct gccacaatcg gcagttctcc gctgcggatc ggccggtcgt cgttccgctc
     1261 gtcgagattc ataacgccgt cttcactaat cctgcgcaaa ggggttgtgg tcgagttctt
     1321 cccgaaccgt cgtggccggt ccgtgacccg gcaacaggat gcaatcgtca ggtaatttca
     1381 acacgcgctg ctgcacggag tccaggtggg ttctgtagag ggtgtgggga ttggaccgcc
     1441 caatcgatcc ggcaaatacg gtgtcaccca cgaagcagac aggctgggac ccggcatcaa
     1501 tccgatagca gataccgcca ggtgtatggc ctggagtagt gagacaacgg acgctcagac
     1561 gcccgactgg gatggtgcgg tcatcgttcg gggcagtaag acgatccttc gaaggcttcc
     1621 aatgcaggag actgacatcg tcagcgccca gataaacggg aacttgccat cgtgccagga
     1681 gttcttgaat cccttcggcg tggtccgcat gcccatgggt gaggcagatt ccggtcagcc
     1741 ggacgcaatg ggattcgagc caagcaatca tggcgggcgc attgtaggcc gtgtcgatta
     1801 acaaggcttc tcccccgtcg tggagaatgt acccctgcac tccatagccg ttgatggagc
     1861 catggaccgt ctcgatccag accggcatgc gttgcgggat cggttcccat ttgtcgatca
     1921 caatctgttc aagcggtgca gatcgcagac ccaatgcctt cgccagtatc tgtacctcag
     1981 cacgatcgtg tggctggtct cctcgttcca gggttgtaat ggcttcggtg gataagcccg
     2041 tcgcacgagc gatgtcaccg accgaaagcc cttgcccggt ccgcgccttc ttgaggatat
     2101 cggaaaagtc gtcttcaagc ggcatgtgaa aagccttcag ctgtcagcag tcagctctca
     2161 ggttcggatt cggtctttgc ggaaacctga aggctgaccg ctatcgattt tgttccacat
     2221 ctacccattc ttagcttagg ccgctaatga tacgtcggta atcgtcctca ggaaatgctt
     2281 ttaacgtcgt ggaacgtaca ttacccagcg atcccagctt gagaccgaaa ctcgctagct
     2341 tgtcgttgtt gggcatatcc acaatgagca ccagatcgta cacccccagc gtcatataaa
     2401 ccgatttgac ctcgccaccc atgacctttg cgagcttctt caccccatcc agccgcttcg
     2461 gcgactcctt cacgttcttg attccgtgat cggtccagtt gagcagcatc acagcggtca
     2521 ccatcggaac ctccttatct actctgtcac tcaaagctcc ggaggcttta cctctctgga
     2581 gcgggtggcg ctgatcaggg cgcccgaccg cccgcagcgg ggtccctaca ccgggcgggg
     2641 taagagggcg cacggcgcga tgaataaaga gcgccacgtt tgtgcgcgcc gccgagccgg
     2701 tgaggcggcc ggaccggtcg ggctggtcgg cgccaggacc cgctcactcc tcagctctcc
     2761 ggagccaggt ttatttcctt tacctccccg aatcccgaca gcagcttcgg cagggcttcg
     2821 ccggagccga ctgccaaaat ggtcagccgc tcagggtgaa aatgcttctt cgccgccgcc
     2881 aggatgtcct ctttcgtcaa tgcgatcacg cgagcacgca attgctggag gaaatccttg
     2941 ggaagcccgt catattccag tg
//
LOCUS       DATMXI010000165         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_185440, whole genome shotgun sequence.
ACCESSION   DATMXI010000165 DATMXI010000000
VERSION     DATMXI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185440"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1007
                     /gene="rph"
                     /locus_tag="VN444_03100"
     CDS             264..1007
                     /gene="rph"
                     /locus_tag="VN444_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWR20833.1"
                     /translation="MNSSTGLLRVDGRRNDQLRPVKVTRNFIKHAEGSVLIEMGDTKV
                     ICTASIEEKVPPFLKGKGQGWVTAEYSMLPRSTHERSPREAVKGKQGGRTLEIQRLVG
                     RALRAVTDMGQMGERSIWLDCDVIQADGGTRTASITGAFIALADACTVLKKKGLIKKR
                     PLTDYLAAISVGKVGGEIFVDLAYTEDSMAEVDMNIVMTGQGRYVEVQGTAERTPFAK
                     QEMDQFMAIGWGGIQTRTALQKDLIGELE"
     gene            1018..1635
                     /locus_tag="VN444_03105"
     CDS             1018..1635
                     /locus_tag="VN444_03105"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HWR20834.1"
                     /translation="MIFELVLATHNRHKATELIQLLGGLGVTIRTLDEFPEAPEVVED
                     GDTCEANAVKKACAIAEYTGLPAVADDTGLEVDALGGRPGVYAARYAGEGATYEDNCR
                     KLLRELMGVPREQRTARFLTVAALALPSDGIRVVRGTLDGLIAEEASGTAGFGYDPVF
                     LIPELGMTLAQLSADHKNRISHRAKAFTKMREIVKRLGQQSFVNG"
     gene            2701..2994
                     /locus_tag="VN444_03110"
     CDS             2701..2994
                     /locus_tag="VN444_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20835.1"
                     /translation="MPFAEAFSSNNAPASAAQGELTLPEYLALPTWIAQALDAPEVSV
                     RLGALDTWAQQGAQAPLDPLIVALDDENDAVREKAMTIIEQHWVVEQDAEPQQ"
BASE COUNT          713 a          774 c          893 g          678 t
ORIGIN      
        1 ggattcatcg ctgcgaccgt gttttcatgt gaggcactcg ccttattact ttggaatctg
       61 gtggatcgtt cggccggagt acgcgcaggt ccgtacacaa ttagccgttc gcgccgcgta
      121 cttttgtaca atcgaccggg aaacgtgaca ggggcaccga gcattccgtc ctgcgctact
      181 cgtgatcagg gcgtgacacc gtcttcattg cgctttaata agtccttcac tataatgcgc
      241 ggcaccatag aggggagtga tcaatgaaca gctcgacggg tctcttgcgt gtcgatgggc
      301 ggcgaaacga tcagctacgt ccggtgaaag tgacgcggaa tttcatcaaa catgccgaag
      361 ggtctgtact catcgaaatg ggcgacacca aagtgatctg cacggcttct atcgaagaga
      421 aggttcctcc cttcctgaag gggaaagggc aaggatgggt gacggcagag tattcgatgc
      481 ttccccgttc aacccatgag cgatctccgc gtgaagcggt caaaggaaaa cagggcggca
      541 ggacattgga gattcaacgt ctcgtaggac gggcacttcg tgcagtgaca gatatgggtc
      601 agatgggaga acggtcaatc tggctcgatt gcgatgtgat ccaggctgat ggaggaacca
      661 gaactgcgtc gatcacaggc gcgttcattg ccttggccga tgcctgtacc gtcctcaaaa
      721 agaagggttt gatcaagaag cgtcctttga cggattacct cgctgccatt agtgtgggaa
      781 aagtcggtgg ggagatcttc gtggatctgg cctatacgga agactcgatg gccgaagtcg
      841 atatgaatat cgtcatgacc ggccaagggc gctacgtcga agttcagggc acggcggaac
      901 ggaccccctt tgccaagcag gaaatggatc agtttatggc cataggatgg ggcggaattc
      961 agactcgcac cgcgctgcag aaggatctga tcggagagct ggaatagggc cgtgagcatg
     1021 atctttgaat tggtactcgc gacgcataat cggcataagg caacagaact cattcagctt
     1081 ttaggaggat tgggcgtcac gattcgaact ctcgatgaat ttccggaggc tcctgaggtg
     1141 gtcgaagacg gggatacttg tgaggccaac gcggtcaaga aggcctgcgc cattgccgag
     1201 tatacggggc tgccggctgt cgccgacgat acagggctgg aagtcgatgc gcttggagga
     1261 cggccgggtg tgtacgctgc ccgttatgct ggcgaagggg cgacttacga agacaactgt
     1321 cgaaagttgc tgcgagagct gatgggcgtg cctcgtgaac aacgaaccgc ccgcttttta
     1381 acagtcgctg cgctcgctct gccttcagac ggaatacggg tggtacgagg gacgttggac
     1441 ggactcattg cagaagaggc aagcggaaca gcgggattcg gatatgatcc tgtgtttctc
     1501 atcccagaat tgggaatgac cttggcccag ctatcggcag atcacaaaaa caggataagc
     1561 caccgcgcaa aggcgttcac caaaatgcga gaaattgtga aacgccttgg tcaacagtca
     1621 ttcgtcaatg ggtgacaggg cctaggatcc tctctcatcg gtaatgaatg ccgatgaatc
     1681 attcttcagt ctggcggggc atagcgcgta aataagagga ttgctgaagt tcttgatcgg
     1741 agcagcaatc ctctgtgttt atacgtagtt cagctctcag atctgtactc tcttgtctct
     1801 cttcatttct gtctaatcat cttggccttt acattaaggc ctcatgctcc tctccttggt
     1861 gcgcgagcaa agataaaaag caaccagacg cctggcgtcg catacgatat ttatactgtc
     1921 cgcgcagaac aagagagagt ggaccccccg ccttcaacaa cacaacccac cacacgaccg
     1981 gctgcgtact caatgctgcg ctgtaccgca ttcgcgtgtc gttggcatcc tgagtgcgca
     2041 tggctcagag aggggattca ggcatcatcc aagaggcgca ccctccagcc agcttccata
     2101 gtgttcctgc aatattggat gtgacgcaca aagattccta gcccaacgat ggccgccagg
     2161 ttgtttggcg caaactggct ctgcaagcca ttttacggca tattctgcgg tgccaatttc
     2221 tgtgctccaa ggggccaatt tcggcacttc ttgggcttag aacaggacaa acccacagta
     2281 acttctccca tcgaattgat ccaagtcgat ggattctcat cgggtttagc cacaccgaat
     2341 ctgaacggtt ccctgatggc atggcgattg cgctacggga gggtaagagt cgcgaaggtt
     2401 cgctaggcag tacgaaaggg ttaatgaaat atgctaggca cgtcatacgt agggaatcat
     2461 gctgtatctt gtcatgtcga tttgtccgtc tctatgtctt ctaacatgtg ttcgtcaaag
     2521 catcttccgc ttcagctctt tccgggcctg gcgtggttct tgtgcatgat ggttctcgca
     2581 ggcctttccg gttgtggacc gggggtatcc gaccacacgt cgaatccgga ggcccgcgct
     2641 agcgtgggaa tgtcatccga gcctaagccc gtttcgcaga acactccgga aacgagccgc
     2701 atgcctttcg ccgaagcgtt ctcgtcaaat aatgcgccag catcagcagc ccaaggagag
     2761 ctgacactgc ccgagtatct ggccctgcct acctggatcg cgcaggcact ggacgctccg
     2821 gaagtctccg tgaggctcgg agccctggac acgtgggcgc agcagggagc ccaagcaccc
     2881 ttggacccgc ttattgtggc tctggatgac gagaacgacg ccgtccggga aaaagcgatg
     2941 acgattatcg aacagcactg ggtggtcgaa caggatgcgg agcctcagca atgaagttat
     3001 gcgaggtaga gagggcagag gcaaggcaag aacgacaaga tgttgaggag ggacgtgt
//
LOCUS       DATMXI010000166         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_203843, whole genome shotgun sequence.
ACCESSION   DATMXI010000166 DATMXI010000000
VERSION     DATMXI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203843"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN444_03115"
     CDS             <1..237
                     /locus_tag="VN444_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20836.1"
                     /translation="VRVSDSGCGIAEADLEKIFLPFFTTKPKGAGLGLAIIERILERH
                     GARVSLESRIGQGTTFTLLFRHRRRDTASSQGEI"
     gene            239..1624
                     /locus_tag="VN444_03120"
     CDS             239..1624
                     /locus_tag="VN444_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR20837.1"
                     /translation="MKPRILVVDDEPLVRTALSETIRRKGCESVMACDGEDALLQFKR
                     FPASLVFADVRMPRMDGLEFLKQIKMVGCPPRVVLITAYGSVETAVQAMKEGAYDFLM
                     KPFSAKRVEDILERAMAAEGPHPDDGPASVIISRAPKILSLLGTIRRVADDAASILIQ
                     GESGTGKELFARQAHLWSKRRRGPFVAVNCAAIPESLLESELFGYERGAFTGAVTRRV
                     GKFEAADGGTLLLDEISEMPAVLQAKLLRALQERTIDRIGGLRPVEIDIRVVATTNRD
                     LKEEIRHGRFRPDLYYRLAVVPISLPPLRERRGDISLLAQHFVMRYAGGSGSVVREIS
                     PAAIKRLEEWPWPGNVRELESCIHRSVLLCDRIRLEPSDLILETLVEPATNDQIQEAA
                     GGSFRAMEKQLILETLAKVGGNRMKAAEILGVSVRTIRNKLRDYREAGKILPMTNRDL
                     AASLASVRESP"
     gene            1787..2149
                     /locus_tag="VN444_03125"
     CDS             1787..2149
                     /locus_tag="VN444_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWR20838.1"
                     /translation="MQLLFDDTSDLLTEMVRAASLRHQVLARNIANIDTPGYRPMEVD
                     FSEELRLASEAGEPPATVVRATVAADRSVGAGRYDGNAVDLDRQMAKMAENALWHNTM
                     IQILNSRMNLLRTVIRGG"
     gene            2159..2590
                     /gene="flgC"
                     /locus_tag="VN444_03130"
     CDS             2159..2590
                     /gene="flgC"
                     /locus_tag="VN444_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWR20839.1"
                     /translation="MGSMFASMEINATALAAQRVRMNLIAENLANAEVTRTPIGGPYR
                     RKGVVLGTRGTSAFEGLLNAGAAPGGVEVLKIVESQAPPTLVYNPSHPDADTAGYVAM
                     PNINPVMEMVDLMSASRAYEANISAIQIAKSMMTKTLEIGR"
     gene            2688..2990
                     /gene="fliE"
                     /locus_tag="VN444_03135"
     CDS             2688..2990
                     /gene="fliE"
                     /locus_tag="VN444_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWR20840.1"
                     /translation="MSSNPILPVRLGQGLPVPSATKTGVSGEGSFAGLLREAVSQTDR
                     LQHEADALVEQVARGQSGDLAATMIAIEKAQISFQLMLQVRNKVVEAYQEIMRMPV"
BASE COUNT          623 a          817 c         1025 g          598 t
ORIGIN      
        1 gtccgggtgt cggatagcgg atgcggcatt gccgaagccg atctggaaaa gatattcctg
       61 ccgttcttta cgacaaagcc gaagggtgcg gggctcggct tagctattat cgagcggatt
      121 cttgagcggc atggagccag ggtaagcctg gaaagtcgga taggccaagg gactaccttc
      181 acgcttctgt tcagacaccg acgccgggat acggcgtctt cccaaggaga aatctgacat
      241 gaagcctcgt attctggtgg tggacgatga gccgttggtg cgaacggccc tgtcggagac
      301 gatccgacga aaggggtgtg aaagcgtcat ggcctgcgac ggcgaggatg cgctgctcca
      361 gttcaagcga ttccccgcct ccctggtctt tgccgacgtc cgaatgccta gaatggacgg
      421 gcttgaattc ctgaagcaga tcaagatggt cgggtgtccg ccacgcgtgg tcctgatcac
      481 agcgtacgga tcggtggaga cggccgtgca ggcaatgaag gaaggggcct atgacttcct
      541 gatgaagccg ttttcggcga agcgggtcga ggacatcctg gagcgggcta tggctgcgga
      601 ggggcctcat ccggatgacg ggcccgcgtc cgtcatcatc agccgtgccc cgaagatact
      661 cagtctgctt ggcactatca ggcgagtggc ggacgacgcg gcgtcgattc tcatccaggg
      721 cgagagcggg acgggtaagg agctgttcgc ccgtcaggcg catctctgga gcaagaggcg
      781 ccgtgggccc ttcgttgcgg tcaactgcgc ggcgatcccg gagtcgttgc tggagagcga
      841 gttgttcggc tatgagcggg gcgcctttac gggagcggtc actcgtcgtg tcgggaagtt
      901 tgaggcggca gacggcggca ccctgctgct cgacgagatc agcgagatgc cggcagtatt
      961 gcaggccaag ctgctcaggg cgctgcagga gcggaccatc gaccggatcg gcgggttgcg
     1021 gccggtcgag atcgatatcc gcgttgttgc gaccaccaat cgggatctca aggaggagat
     1081 ccgtcatggg cgttttcgtc ccgatctgta ctaccgtttg gcggtcgttc cgatctccct
     1141 gcctccgctt cgagagcgga gaggcgacat ctcactgttg gcgcaacatt ttgtcatgcg
     1201 gtatgcgggc gggtcgggca gtgtggtgcg ggagatctcg cccgccgcca tcaagcgatt
     1261 agaggagtgg ccgtggcccg gcaatgtgcg ggagctcgag agctgtattc accggtcggt
     1321 cctgctgtgc gatcgaatcc gcctcgagcc gtcggatctg atattggaaa ctctggtcga
     1381 gcctgcaacc aacgatcaga ttcaggaagc ggcaggcggc tcgttccgcg ccatggaaaa
     1441 gcaattgatc ttggagacgc tggcgaaagt aggcggcaat cggatgaagg cggcggagat
     1501 tctcggcgtg agcgtgcgga cgattcgaaa taagctgagg gattatcgcg aggccgggaa
     1561 aattttgcct atgaccaatc gggatcttgc cgcctctttg gcttcagttc gggaaagccc
     1621 gtgaaaaaca gggctttccg catggcacac cctttgctgc ttcgagaggc tgaaggttga
     1681 taggctgaag gctgttaggt aagtcccctt tagccttcgg tcttcagtct gaactcctaa
     1741 ctgctgagtg gtgaccgcta aacgcctttc taaggaagga ttacggatgc aactgttgtt
     1801 cgacgacacc tcagacctgc tcaccgagat ggtccgagct gcgtcgctgc ggcaccaggt
     1861 gctcgctcgg aacattgcga atatcgacac cccgggctat cgcccgatgg aggtggactt
     1921 cagcgaagag ctcagacttg cgtctgaggc gggcgagcct ccagctacgg tcgtcagggc
     1981 caccgtggcg gccgatcgat cggtcggagc aggacggtac gacggcaatg cggtcgatct
     2041 cgatcggcag atggcgaaga tggcggagaa tgcattatgg cacaatacga tgattcagat
     2101 cctgaactca cggatgaatc ttctgcgaac cgtcattcgg ggcggataag gagacggcat
     2161 gggatcaatg tttgcgtcga tggagatcaa tgcgacggcg ttggcagccc agcgggttcg
     2221 gatgaacctt attgccgaaa acctcgccaa cgccgaggtc acgcggacgc caatcggcgg
     2281 accgtatcgc cgcaagggag tggtgctcgg cacgcgcggg acgtcggcct ttgagggact
     2341 cttgaacgcg ggcgccgcac ctggcggggt cgaggtgctg aagatcgttg agagccaggc
     2401 gccgccaacc ctggtctata acccgtcgca tcccgatgcc gacaccgcgg ggtacgtggc
     2461 gatgccgaat atcaacccgg tgatggagat ggtcgatctg atgtcggcat cccgcgccta
     2521 tgaagcgaat atctcggcga tccagattgc gaagagtatg atgacaaaga cgttggagat
     2581 aggcaggtga gaatcagcgt tcagccctca gcgatcagca gccagcggtc ggttctccga
     2641 cggatgaaag ccgatagccg gacgctgatc gcttaaggag tcatggcatg tcaagcaatc
     2701 cgatcttacc ggtaaggcta ggccagggat tgcccgtccc atctgcgact aagacgggcg
     2761 tcagcggcga aggctcattc gccggcctct tacgggaggc cgtgagccag acggaccggt
     2821 tgcagcacga ggccgacgcc ctcgttgaac aggtggccag agggcagtcg ggcgacctcg
     2881 ccgcaaccat gatcgccatc gagaaggcgc agatctcatt tcaactcatg ctccaggttc
     2941 ggaataaggt cgtcgaggcg taccaggaga ttatgcggat gccggtatag agtggaggct
     3001 ggggacgcgc gtgcgaccgt atcccctgac aacagatgcc tgatgccatc acaaaggttg
     3061 ttg
//
LOCUS       DATMXI010000167         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_320001, whole genome shotgun sequence.
ACCESSION   DATMXI010000167 DATMXI010000000
VERSION     DATMXI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320001"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1702)
                     /locus_tag="VN444_03140"
     CDS             complement(398..1702)
                     /locus_tag="VN444_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWR20841.1"
                     /translation="MNAIDAIDAIAVIGLGYVGLPLATAFAQHLPTIGFDVNPKRIQA
                     LRQGADWNGELSANALSAPHLRLTDDPSHLQEASFLIVAVPTPIDRAKRPDLGPRIEA
                     SRLVGRHLSPGAIVVYESTVYPGCTEEVCIPVLERESGLKAGRDFTVGYSPERINPGD
                     PVHTLERVVKVVASQDEATTAMMARVYGLVVRAGIHTAPDIKTAEAAKVIENIQRDLN
                     IALMNELALLFHRLGLDTHEGLKAAGTKWNFLPFDPGLVGGHCIPIDPYYLTHKAEEV
                     GYHPDVIMAGRRINDAIGRYVARETVRLLIQVGKVIKGARVLVLGLAFKEDVRDARNT
                     RVLELVDELTQHGLDVAVTDPVVGSPDIQRAGLTEAPDPFGAASRYDALILAVPHQVF
                     RDQSVDAYLRLLNDDNGPGVMVDVKGVFRDALRGTKHLHWSL"
     gene            complement(1799..2776)
                     /locus_tag="VN444_03145"
     CDS             complement(1799..2776)
                     /locus_tag="VN444_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWR20842.1"
                     /translation="MKRALVCGAGGFIGGHLVKKLKREGYWVCGVDIKTHEFVPTQAD
                     GFRLLDLRDPQNCRTALTLSDGGSFDEVYQLAADMGGMGFISRAECEVLHNNALINIH
                     MAHTAAEMGVPRYFFSSSVCIYRDMQPDEPPVTEDDAVPANPDNEYGWEKLYAERVAM
                     AYGRRYPMQVRIARFENCYGPEGTWTGGREKAPAALCRKVAEAEDGGTIEVWGDGTAI
                     RVFTYVDDLVDGIFALMHSDMDGPVNLGSEERVTVADLVRTVIEASDKRMTVKYVPGP
                     VGVHARNFSKARIASLGWKPNVSLREGIARTYPWIQAQVSVARGSAHRA"
     gene            complement(2856..>4197)
                     /locus_tag="VN444_03150"
     CDS             complement(2856..>4197)
                     /locus_tag="VN444_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWR20843.1"
                     /translation="TVEPWSIAVAEIGIYTIAVVWGLAMVSAGEIRIERTALNLCWLL
                     VLIFGLLQVIPLPFSIIRMISPKAAALYEQMGFDSHLVASWHTLSLVPYATRQALMRL
                     LALALLFWVVVNHLQTREQVDRIVRVVMMTGFGLALFGIIQHFAGNGKLYWVRELTQG
                     GSLFGPYVNHNHFAGYMEMVIPLTIGYIVANRRPISNSAEGWRIRLLRWGTPQASRSL
                     LAFFGGIIMSVALLLTGSRAGLFSFFCSILFMGFLLSARRLRSKRLWGMLASFVAVGL
                     TYALWLNPDRVLQTFAILWRGTDDPSFQGRILVWQDTLRLGDDFRWSGTGLDTYLWAF
                     PLYKQPLIGQAVYDYAHNDYLQAFAEGGLPLVAIFALALLWGGTQLLNGWSQHEGTHT
                     RGIGLGLLAGLVAMLVHSAYDFNLHILANAILFVLLLALASRVLLLRRSRVDAR"
BASE COUNT          841 a         1311 c         1247 g          798 t
ORIGIN      
        1 actcttgcca ttatgaagag acaccgcctg acgcatatcg caacgaatga tcctgatttt
       61 gaacgggttg agtttctcaa ggtctggaaa ccctaacctc tatcgttctc tgctagttcc
      121 atctacaaca ccttatccac aatctgagtt ccgctccgaa gtgcctgagg cgggtgttca
      181 ggctaccggc aaggatgacg cgtttcatgc gtcgatgtcg tcgatggcgt gcggcgttta
      241 tggcgtctat agcgtcgatt gcggcggagc gtcattgcgg ggattgcgtc tgttgcgtct
      301 attgcgtgcg gcgttgattg cgtccatggc gtcattgcgt ctgttgcgtc gattgcggct
      361 ggttggtcgg tgacgagaga gacggaatag acccaagtca gaggctccag tggaggtgct
      421 tggtgccgcg gagggcgtcc cggaacacgc ccttgacatc gaccattaca cctgggccgt
      481 tgtcatcatt cagcagtcgc agataggcat ccaccgactg gtctcggaag acctggtggg
      541 ggacggccag gatcagggca tcatagcggc tggccgcccc gaagggatcc ggcgcctccg
      601 tgagtccagc ccgttgaatg tcgggtgagc cgacgacggg gtcggtcacg gccacgtcca
      661 gcccatgctg ggtcagctcg tcgacgagct ccagcacccg cgtgttgcgg gcatcccgaa
      721 catcctcctt gaaggcgaga ccgaggacca ggacccttgc ccccttgatc accttgccta
      781 cctggatcag gagcctgacc gtttcccgcg ccacataccg cccaatcgca tcgttgatcc
      841 gccggccggc cataatcaca tcagggtggt agcccacctc ttccgcttta tgggtgaggt
      901 agtagggatc gatcgggata cagtggccgc cgaccaggcc gggatcgaag ggcaagaagt
      961 tccatttggt gcccgcggcc ttgagcccct cgtgggtgtc caggccaagg cggtgaaaca
     1021 ggagggccag ctcgttcatc agggcgatgt tcaggtcccg ctggatgttc tcgatgactt
     1081 tggcggcctc agcggtcttg atgtcgggag ctgtgtggat gccggccctg accacgaggc
     1141 cgtagactct ggccatcatg gcggtggtcg cctcgtcctg tgaggccacc accttgacca
     1201 cccgttcaag ggtgtggacg ggatcgcccg ggttgatccg ctcgggtgag tagcctacgg
     1261 taaagtcgcg gccggccttc agcccggatt cgcgctctaa caccgggata cacacctctt
     1321 cggtacagcc tggatagacg gtagactcgt acacgacgat cgccccgggg ctcaggtgcc
     1381 gccctaccag gcgactcgcc tcgatgcggg ggccaaggtc cgggcgcttg gcccgatcga
     1441 tgggggtcgg cacagccaca atgaggaagg aggcctcttg caggtgcgat ggatcgtccg
     1501 tcagacgtag atgcggggca ctgagcgcat tagcgctcag ctcgccattc cagtcagcgc
     1561 cttgtcgcaa ggcctggatc cgcttcgggt tcacatcgaa gccgatggtg ggtaggtgct
     1621 gggcaaatgc cgtcgccagc gggagaccca cgtaccccaa gccgatgacg gcgatggcgt
     1681 ctattgcgtc gattgcgttc atggcgtcga ttgcgtcgtt gcgtcatcgc gttgattacg
     1741 taccgcgttt gttgcgccta tctgatcgat tacgtctgtt acgcggtttg tcctcggctc
     1801 aagcgcggtg cgctgagcct cgcgctacgc tgacctgcgc ctggatccac ggataggtac
     1861 gcgcaatccc ctcacgaagc gacacgttgg gcttccaccc cagcgacgcg atacgcgcct
     1921 tgctgaaatt gcgggcatgc actcccacag ggcctggtac gtacttgacg gtcatgcgct
     1981 tgtcggatgc ctcgatcacc gtccgtacca gatcagccac cgtcacccgt tcttcactcc
     2041 ccaggttcac ggggccgtcc atatctgagt gcatcagcgc gaagatcccg tccaccagat
     2101 catccacata ggtgaacacc cggatggccg tcccatcacc ccaaacctcg atggtcccgc
     2161 catcctccgc ctccgccacc ttccggcaga gggccgccgg cgctttttcg cggccgccgg
     2221 tccaggttcc ttccggaccg tagcagtttt cgaagcgagc gatgcgcacc tgcatggggt
     2281 aacggcgccc gtaggccatt gccactcgct cggcgtagag cttctcccaa ccgtactcgt
     2341 tgtccgggtt ggccgggacg gcgtcgtcct cggtcacggg cggctcgtcg ggctgcatgt
     2401 cgcgatagat gcataccgac gaagagaaga agtagcgagg aacgcccatc tcggccgcag
     2461 tgtgagccat gtggatattg atcaaggcgt tgttgtgcaa cacctcgcac tccgcccggg
     2521 agataaatcc catcccgccc atatcggcag cgagttggta gacctcatcg aagcttccgc
     2581 cgtctgacag agtcagggcc gtccggcagt tctgggggtc gcgcaagtcc agcagccgga
     2641 acccatcggc ctgggtgggc acaaattcgt gcgttttaat atccacgccg cacacccagt
     2701 agccctcgcg cttaagcttt ttcaccaggt gcccaccgat gaacccgccg gcaccgcaga
     2761 ccaaggctcg cttcatgtct tgcgtctcct ttgttgtaga gcaacgctcg cgaatacctc
     2821 cgcgacgcaa tgaacgcaat agacgccgca cgcaatcacc gggcatcaac ccgtgaacgc
     2881 ctaaggagga gaacccggga cgccagcgcc aacaggagaa cgaaaaggat ggcgttcgcc
     2941 aggatgtgga ggttaaaatc gtaggcgcta tgaaccagca tcgctaccag cccggccagc
     3001 agaccaagtc cgatcccccg cgtgtgcgtt ccttcatgct gggaccaccc gttcagcaac
     3061 tgcgtcccgc cccacagcaa tgccagcgcg aagatcgcca ccaagggcaa tcccccttcc
     3121 gcgaatgcct gcaggtagtc gttgtgggcg tagtcgtaca cagcctgacc gatcaggggc
     3181 tgcttgtaga gcggaaaagc ccagagatat gtgtccaggc ccgtgccgct ccagcggaag
     3241 tcatcgccca ggcgcaacgt atcctgccag acgaggatac gaccctggaa ggagggatcg
     3301 tcagtcccac gccacagaat cgcaaaggtc tgcaggaccc tgtccgggtt cagccagaga
     3361 gcatacgtca gccccaccgc cacaaatgag gccagcatgc cccatagccg tttactgcgc
     3421 aatcgccggg cagagagcag gaagcccatg aagaggatgg agcagaagaa gctgaacagc
     3481 cctgctcgtg atccggtcag cagcagagct acagacatga tgatcccccc aaaaaacgca
     3541 agcagcgacc ggctcgcctg cggtgtgccc caacgaagca accggatccg ccagccttcc
     3601 gcactattag atatcggtcg cctattcgcc acgatatagc cgatcgtcag cggaatcacc
     3661 atctccatgt aaccggcgaa gtgattatga ttgacatagg gtccaaagag gctgccgccc
     3721 tgggtcagtt cccgaaccca atacagcttg ccgttcccgg caaaatgctg aatgatcccg
     3781 aaaagcgcaa gcccgaatcc cgtcatcatc accacgcgaa cgattcgatc gacctgctca
     3841 cgggtctgca ggtggttgac gacgacccag aagaggagcg ctaaggccag gagcctcatc
     3901 agcgcctgtc tggtcgcgta cggcacgagt gagagcgtat gccatgaagc cacaagatgg
     3961 ctgtcaaacc ccatctgctc gtagagcgcg gcggctttcg gcgagatcat gcgaatgatg
     4021 gaaaacggaa gcgggatcac ctgcaacagt ccgaaaatca gaaccagaag ccagcacagg
     4081 ttcagggccg ttcgctcgat gcggatttcg ccggcgctca ccatggcgag tccccacacg
     4141 actgcgatcg tgtagatccc tatctccgcg acggcgatcg accagggctc gacggtt
//
LOCUS       DATMXI010000168         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_154507, whole genome shotgun sequence.
ACCESSION   DATMXI010000168 DATMXI010000000
VERSION     DATMXI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154507"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="VN444_03155"
     CDS             <1..84
                     /locus_tag="VN444_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWR20844.1"
                     /translation="AMEKELRFAIREGGRTVGAGVVSEVVD"
     gene            187..336
                     /gene="rpmG"
                     /locus_tag="VN444_03160"
     CDS             187..336
                     /gene="rpmG"
                     /locus_tag="VN444_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWR20845.1"
                     /translation="MREIITLACSECKRRNYTTTKSKQNTPDRLELSKYCRWCRKHVA
                     HKEAK"
     gene            406..481
                     /locus_tag="VN444_03165"
     tRNA            406..481
                     /locus_tag="VN444_03165"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Trp,seq:cca)
     gene            646..840
                     /gene="secE"
                     /locus_tag="VN444_03170"
     CDS             646..840
                     /gene="secE"
                     /locus_tag="VN444_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWR20846.1"
                     /translation="MRERWQQLVQFLQEVRTELKKVHWPTRKEVVGSTVVVIVSVVIV
                     SFFLGGIDVILQRLLTLVIG"
     gene            872..1402
                     /gene="nusG"
                     /locus_tag="VN444_03175"
     CDS             872..1402
                     /gene="nusG"
                     /locus_tag="VN444_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWR20847.1"
                     /translation="MPQSWYVIHTYSGFENKVKGSIEQRAAALGLSDKISQVVIPTED
                     VVELKKGKRTVSSRKFLPGYVLIKAEMSEDIWYLVKNTPKVTGFVGPATQPTPVPEEQ
                     VQAILQQVEEGAERPKHRVMFLRGESVRVIDGPFANFTGLVDEVKPEKGRLKVMVSIF
                     GRPTPVDLEFLQVEKV"
     gene            1452..1874
                     /gene="rplK"
                     /locus_tag="VN444_03180"
     CDS             1452..1874
                     /gene="rplK"
                     /locus_tag="VN444_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWR20848.1"
                     /translation="MAKKVTAIVKLQVPAGKANPAPPVGPALGQHGVNIMEFCKAFNA
                     QTASEEGLVIPVVITIYADRSFSFITKTPPAAILLKQAVGIAKASKEPNRTKVGKVTR
                     KQVEEIARTKMPDLNALSLESAVKIIEGTARTMGIEVV"
     gene            1986..2693
                     /gene="rplA"
                     /locus_tag="VN444_03185"
     CDS             1986..2693
                     /gene="rplA"
                     /locus_tag="VN444_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWR20849.1"
                     /translation="MSKVGKRYGVATEAVTRVGSCDLAKAIEAVKSNAGAKFDETMEI
                     AVRLGVDPKHADQMVRGTVVLPHGTGKSVRVLVFMKGEQEKDAREAGADYVGCEDLIE
                     KIQQGWLEFDRAIATPNVMGLVGRLGKVLGPRGLMPNPKTGTVTFDVGKAVREFKGGK
                     IEYRTEKAGIVHAPFGKASFTADQLRENAVALLEALLRAKPASSKGRYLNGVTISSTM
                     GPGIKVELDTLVGLAKS"
     gene            2724..3239
                     /gene="rplJ"
                     /locus_tag="VN444_03190"
     CDS             2724..3239
                     /gene="rplJ"
                     /locus_tag="VN444_03190"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWR20850.1"
                     /translation="MNQAEKAAVVDELRTGLAGATVAMLADPRGLSVSDLTELRKLLR
                     QQGVALRVVKNTLARLAITGTELQELKPYLVGPTAIVHGKGDPTVPVKLLASFIKTKS
                     TFQIKAGFAEGRVLASQEVMVLADLPPREVLAARLAGILQSPLRGLVAVLSGPLRSLL
                     MVLEAVKQQKE"
     gene            3317..3709
                     /gene="rplL"
                     /locus_tag="VN444_03195"
     CDS             3317..3709
                     /gene="rplL"
                     /locus_tag="VN444_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWR20851.1"
                     /translation="MAKVTADDVLDSIEQWSVLDLNKLVKGIEEKFGVSATAMMPAMA
                     VGQGGASVAAAAPTEEKTEFSVILASVGEKKIQVIKEVRAITGLGLKEAKDLVEGAPK
                     PVKEGLSKAEAEAIKTKLEATGATAELK"
     gene            3736..>4819
                     /locus_tag="VN444_03200"
     CDS             3736..>4819
                     /locus_tag="VN444_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWR20852.1"
                     /translation="MALVKKSVAAERWDFSKIKEVISIPNLIEIQRRSFDQFLQMKVP
                     PHAREEIGLQGAFASIFPIFNYDSSASLGFVKYEFGVPKYGAEESLEKGMTYSVPLKV
                     TLRLMVWDKPVSSEASSIRDIKEQEVYLGEMPLMTPQGTFIINGTERVVVSQLHRSPG
                     VFFDHDNGKTHPSGKILYSARLIPYRGSWLEFEFDSSDVLHVRVDRRRKFLASILLRA
                     IGYGSNEEILNLFYERDVLRIEKGKDFLLHVDSPGATSFRVSRDIPDPHTKQLILGAT
                     KRITKVAQKRLQALKITEVPLYKEDLIGRIAAADIVDTKSGEVILECAQEITEEVLEQ
                     VLKSELSSFAVLASRVGQDMFEIRENI"
BASE COUNT         1172 a         1110 c         1501 g         1036 t
ORIGIN      
        1 gccatggaga aggagctgcg tttcgccatc cgcgaggggg gccgcacagt tggggccggc
       61 gtggtgagcg aggttgtaga ttaggggtct atctggtcca tttcgtctat ctggttgaga
      121 ccgaacagac cgaatggacc aaacgacgag acggaccaaa cgactaaata gacgtacgga
      181 gcatcgatgc gtgagataat tacattagcc tgtagcgaat gtaagaggcg aaattacaca
      241 acgacgaaga gtaaacagaa tacgcccgat aggctcgaac tgagtaagta ctgtcgttgg
      301 tgccgtaagc acgtcgccca taaagaagcg aagtaaaaat gttccaattt ccaggctcca
      361 ggtcgattgc tgagacgccg gggcatggaa tctggaactt ctgagaggcc agtagctcta
      421 acggtagagc accggactcc aaatccgggt gttgggggtt caaatccctc ctggcctgcc
      481 acatgatagc gtgaggacct ttcgcagttc tagcggggga atttttggta gcgggtcggt
      541 ggtattagat tcaccctccg aacaccgcag atctgtgtgt aaggtctttg gaattggagt
      601 gagatttccg ctgaagtcgg agagctagag gagcagaaag agacgatgag ggaacgatgg
      661 cagcagttgg ttcaattctt acaagaggtc aggactgagc tgaagaaggt ccattggccc
      721 acaaggaaag aggttgtggg ctctaccgtt gttgtcatcg tgtcagttgt tattgtctcg
      781 ttctttcttg ggggtataga cgtcatattg cagaggctgc tcactttggt gatcgggtag
      841 cgaggagtag ccgatgccag caggtcagac tatgccgcaa agttggtatg taatccatac
      901 gtattcgggt tttgagaata aggtgaaggg gagcattgag cagcgggctg ccgccttggg
      961 gctatctgac aagatctccc aggtcgtcat tcccactgaa gatgtcgtcg agctcaagaa
     1021 gggaaaacga acggtctcct cgcggaaatt tcttccaggg tacgtcctga tcaaggcaga
     1081 aatgtcggag gatatttggt atctcgtgaa gaataccccg aaagtaactg gctttgttgg
     1141 cccggctacg caaccaactc cagttccaga ggaacaggtg caagccatcc tgcagcaggt
     1201 agaggagggg gccgagcggc cgaaacatag ggtcatgttc cttcgtggag aaagtgttcg
     1261 agttatcgat ggtccgttcg ccaacttcac tggcctcgtt gatgaggtga agccagaaaa
     1321 ggggcggcta aaggtgatgg tcagcatctt cggaaggcca accccggtgg accttgagtt
     1381 cctgcaggtt gaaaaggtct gaaagaacag ctcacagtta atggctaaca gcttctgcat
     1441 ttgaggtcct tatggcgaaa aaagttacag cgatcgtaaa gcttcaggtc cctgccggga
     1501 aggccaaccc agcgccgccg gtcggccctg cactcggaca acatggcgtc aacattatgg
     1561 agttctgcaa ggcctttaat gcacagacgg catcggaaga aggcctggtg atccccgttg
     1621 ttatcactat ctatgcagac cggtccttca gtttcatcac caagactccc cctgcggcaa
     1681 tccttttaaa gcaggccgtt gggatcgcga aagcctcgaa ggaaccgaac cgtacgaagg
     1741 taggaaaggt gaccaggaag caggtggagg agattgcgcg aacgaagatg ccggacttga
     1801 atgcgctatc acttgagtcg gctgtcaaga ttatcgaggg aacggcgagg accatgggga
     1861 ttgaggtagt ctaagaacag ggttgagggt cttaagggtt tagggtttca ggggaacgcg
     1921 atccgcctct tctcctctat accctgcacc cttcaccctg ataccctttc tttcaggggt
     1981 gggatatgtc gaaagttgga aagcgctatg gtgtggccac tgaagcagtg acacgggtgg
     2041 gctcgtgcga cttggctaag gcgatagagg cagtaaagtc gaatgccggc gcgaagtttg
     2101 atgagaccat ggaaattgct gttcgcctgg gggttgatcc gaagcatgct gatcagatgg
     2161 tgagaggcac agtcgtattg ccccatggaa cagggaagag tgttcgagta cttgtcttca
     2221 tgaagggcga acaagagaaa gatgctcgtg aggctggtgc cgattatgtt ggctgcgaag
     2281 acctgataga gaaaattcaa cagggatggc ttgagttcga ccgggcaatc gctaccccga
     2341 acgtcatggg tctagtggga cgtctcggaa aggttctcgg acctcgggga ttgatgccta
     2401 accctaagac cggcaccgtc accttcgatg ttggaaaagc ggtcagggag tttaagggtg
     2461 gaaagattga gtatcgcacg gagaaggcag ggatcgtgca tgcgccgttc ggtaaggcct
     2521 cctttacggc ggaccagctc cgtgagaatg ccgtagcatt gctggaggcg ttgctccgag
     2581 ccaagccggc ctccagtaag ggtcgatacc tcaatggggt caccatatcg tcgacaatgg
     2641 gaccgggtat aaaggtggag cttgacacct tagtggggtt ggcaaagagc tagggaagcc
     2701 accttgcctt gcaggagggg acagtgaatc aggcggaaaa ggcagcggta gttgatgaac
     2761 tcaggaccgg gctagcaggg gctacggtcg ccatgttggc agatcctcgg gggctcagtg
     2821 taagcgattt gactgaactg agaaaactgc tccggcagca gggggttgct ctccgggtcg
     2881 ttaagaatac tctcgccaga ttggccatta caggaacgga actccaggag cttaagcctt
     2941 accttgtcgg cccgacagcg attgtccacg ggaaaggtga tcctaccgtg ccggttaagc
     3001 tcttagcctc tttcattaag acgaaatcca ccttccaaat caaagccggt ttcgcggagg
     3061 gaagggtgct ggccagccaa gaggtaatgg tcctcgccga tctcccccca cgtgaggtgc
     3121 ttgcggccag gctcgctggt atcctgcaat ccccccttcg cggcctcgtg gcggtgcttt
     3181 ctgggccgct tcgttcgctt ctgatggtcc tggaggcggt gaaacaacag aaagagtaaa
     3241 gaaccggccc tggccctaaa gtggcccggg catcgtcgac acatgtggta ctgcgtagtc
     3301 aaggagagga gtgacgatgg caaaggtcac ggctgatgat gtattagact cgattgagca
     3361 gtggagtgtc ttggacctga acaagctggt gaaggggatt gaggaaaagt ttggcgtttc
     3421 cgctacggcg atgatgcctg cgatggcggt tggccaagga ggggcttccg ttgctgcagc
     3481 ggcaccaact gaggaaaaga cggaattctc ggttatcctt gcttctgtgg gggaaaagaa
     3541 gattcaggtg atcaaggagg tgagagcaat caccggcctt ggcctgaaag aggcaaagga
     3601 cctggtcgag ggcgctccta agccggtaaa ggaagggttg tcgaaagctg aggctgaagc
     3661 gataaaaacg aagctcgagg cgaccggcgc gactgcagaa cttaagtaaa gcaggaagaa
     3721 aaggagttag gacgtatggc cctcgtaaag aagagcgtgg cagccgagcg ctgggatttt
     3781 agcaaaatca aagaggtgat ctctatcccg aatctgattg agatccagcg gcggtccttc
     3841 gatcagttcc tgcaaatgaa ggttccaccg catgcgcgag aggagatcgg tcttcaaggg
     3901 gccttcgcca gtatcttccc gatttttaat tacgacagtt cggcgtccct gggttttgtc
     3961 aagtacgagt tcggagtgcc caagtacggc gcagaggaat ccctggagaa ggggatgacg
     4021 tactccgttc cgctgaaggt gacgcttcgc ctgatggtct gggataagcc agtcagctcc
     4081 gaagccagca gcatcaggga cattaaggag caagaggttt acttgggtga gatgcccttg
     4141 atgacgccgc agggtacctt cattatcaat ggaacggagc gggtggtggt cagtcaactc
     4201 caccgctcac cgggggtctt cttcgaccat gacaacggta agactcaccc gagcggcaag
     4261 atcctctact ccgctcggct cattccatac cgaggttctt ggctggaatt cgagtttgat
     4321 tcgagcgatg tactccatgt ccgggtggac cgaagacgga agtttctagc ctcgatcctg
     4381 ctgcgggcga tcggctacgg gagtaatgag gagatcctga acctcttcta tgagcgggat
     4441 gtattgcgca tagagaaggg gaaggatttc ctgcttcatg tggattcacc tggcgcgacc
     4501 agctttcggg tgagcaggga tattcccgat ccccacacga agcaactgat tcttggggca
     4561 accaagcgaa tcacgaaggt ggcccagaag cgactgcagg ctttgaagat cacggaagta
     4621 cccctgtata aagaagatct catcggccga attgcagccg ctgatattgt ggacacaaag
     4681 agcggcgagg tcatccttga atgtgcgcag gagatcacag aagaagtgct ggagcaggtc
     4741 ctcaagagcg aactgagcag cttcgccgtg cttgccagcc gagttggtca ggatatgttc
     4801 gagattcggg agaacatta
//
LOCUS       DATMXI010000169         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_99814, whole genome shotgun sequence.
ACCESSION   DATMXI010000169 DATMXI010000000
VERSION     DATMXI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99814"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VN444_03205"
     CDS             <1..627
                     /locus_tag="VN444_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWR20853.1"
                     /translation="ALVTVEIEAPSSLRAEAGERAIRLSWVAPTRRVDASPLAMVPRY
                     NIYRGTSPGRYDPFPINRTPVRSTRFQDAGLINDQTYYYSVMAVESQEPPWQEGLSSS
                     EVSAAPVDLTPPAPPRGVRAVTGPGAVVSLSWELNRELDLLGYLVYRSDAAERLPQRL
                     TEAPLKSPILTDRSVRSGGRYTYTVTAVDASRRRNESVASAAIEVRVP"
     gene            complement(677..1345)
                     /locus_tag="VN444_03210"
     CDS             complement(677..1345)
                     /locus_tag="VN444_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009916 - alternative oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative oxidase"
                     /protein_id="HWR20854.1"
                     /translation="MGSNLKKLTAEQRRQAQQVTLRSPRMRYGILTRLLFMTMDLVYG
                     RKKTFSKFKVLELIARVPYQSWENVAYVAITHMYAQRDFARRVFDRVKEAREAQDNEM
                     WHLLILEELTHARGMKENMLLYRIIPQVIAFTYYHICWFLYVIKPSWSYLVNAQFEDH
                     AEHEYMEFVAENPQFESEPFKSRFEEDYASSESVADIFRQIGHDERMHKEKSLEWIAT
                     ARFQ"
     gene            complement(1538..1828)
                     /locus_tag="VN444_03215"
     CDS             complement(1538..1828)
                     /locus_tag="VN444_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWR20855.1"
                     /translation="MYWDVKTVKPLSDYRIFVETGDGRRGIFDVKPYLNHGVFRELRD
                     THYFNQVGILFGAVTWPHAQDIAPETLLAEMIPVESMPNEMLQTDPPQAAHR"
     gene            2229..>2642
                     /locus_tag="VN444_03220"
     CDS             2229..>2642
                     /locus_tag="VN444_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWR20856.1"
                     /translation="MKHIIIGTAGHIDHGKTSLVKALTGIDTDRLKEEKERGISIELG
                     FANLALSDDLQLGIVDVPGHERFVKTMLAGVGGIDLVVLVISADEGVMPQTREHLHIC
                     ELLQVKRGVVALTKADLVESDWLEMVQADLKGFLAG"
BASE COUNT          576 a          740 c          771 g          555 t
ORIGIN      
        1 gcccttgtca ccgtcgagat tgaggccccc tctagcctac gggcagaggc cggcgaacga
       61 gcgatccgcc tctcatgggt tgcgccgacc cggcgagtcg atgcaagtcc gctggccatg
      121 gtcccaaggt ataacatcta ccggggcacc agcccaggcc gatacgatcc attcccgatc
      181 aatcggacgc cggtccggag cacgcggttt caggatgcgg gtctaattaa cgatcagacg
      241 tactactaca gcgtgatggc ggtggagagt caggagccgc cctggcagga gggattgtcg
      301 tccagcgagg tgagcgccgc gccggttgat ctgacgcctc cggcccctcc ccgaggtgta
      361 cgagcggtga ctggacctgg tgcggtagtc tcgctgagct gggagctcaa ccgggaattg
      421 gacctgctcg gctatctggt ctatcgaagc gacgccgcag agcgtcttcc ccaacgactg
      481 acagaagccc cgcttaagtc gcccatcctg accgaccgat cggttcgatc aggaggtcgg
      541 tacacctaca cggtcacggc cgttgacgct tctcgtagac gcaacgaaag cgtcgcctcg
      601 gcagccatcg aggtccgcgt cccctaaagc atttcccatt ggcgatgacg ccctctcctg
      661 cagagccgct caccgttcac tgaaatcggg ctgtcgcgat ccattccaaa ctcttttcct
      721 tgtgcatccg ctcgtcatgt ccgatttgac gaaatatgtc cgctacgctc tcggaggagg
      781 catagtcctc ctcaaagcgg ctcttgaaag gctccgactc gaactggggg ttttccgcca
      841 cgaattccat gtattcgtgc tcggcatggt cctcaaattg ggcgttcacc agataactcc
      901 acgacggctt gatgacgtag aggaaccagc agatgtgata gtaggtgaag gcgatcacct
      961 gagggatgat gcgatagagg agcatatttt ccttcattcc gcgggcgtgg gtgagttcct
     1021 ccaggatgag caagtgccac atctcgttat cttgagcctc gcgggcctcc ttgacccggt
     1081 cgaagacccg gcgggcaaaa tcacgctgtg cgtacatgtg ggtaatggcg acgtaggcca
     1141 cgttctccca ggactgatac gggacgcggg cgatgagctc cagtaccttg aatttgctga
     1201 acgtcttttt gcgaccatat acaaggtcca tcgtcatgaa gaggaggcgg gtcaagatac
     1261 cgtatcgcat cctcggagag cgaagcgtga cttgctgcgc ttggcggcgc tgttcggccg
     1321 ttaatttctt caggttcgaa cccatagcaa gacctccgtc gatattgcca acatcggtga
     1381 tatatatcca caaaacaggg aaataagatg tcccccgaac tgaaccctca ggctcttctc
     1441 ttgggatcgg ccataaaccc aggaggtcac cgatgctcag gaaggaggac tggatgatga
     1501 ttcaagcaca gttggagcga ggcatctacg tcgtagctca gcggtgcgcc gcttgcggcg
     1561 ggtccgtctg gagcatctcg ttaggcatgg attcgacggg tatcatttca gcaagcaagg
     1621 tttcaggagc gatgtcctga gcgtgaggcc aggtaactgc gccgaatagg atacccacct
     1681 gattgaaata gtgtgtatcc cgaagctcgc gaaaaacacc gtgattcagg taaggcttca
     1741 cgtcgaaaat cccccgccgc ccgtctccag tctcaacgaa gatgcggtag tcggataatg
     1801 gtttgacggt tttgacatcc caatacatag gacacctcac aggggagcaa tcttgtaagg
     1861 cgtttcgcca ttgacggcca gtttcgtagt tccgtaggtt gggttagccc aagaggcgta
     1921 acccaacaaa caaaatcttc accgcggagg gagaacgcgg agaaaatgat aacaatttgc
     1981 ttttgcagcg ggcggctcta acacctcaac gaaccgcccc ttgctaccca aaaagtagtc
     2041 tcagtctggt caactcttgt ccctcttgtc aagcaaatag tcgtggctta ttctcaaacg
     2101 acgttccccg atcaggcgag gagtgtaggt tacaaccacg aatctgcaaa gtctcgatgt
     2161 aacgaatcgc ttgcgggact tcaaccttgc tgccgcgaag ccccgtatgt tacgatgacg
     2221 gtgtgacgat gaagcatatc atcatcggga ctgcaggcca catcgatcat ggcaagacct
     2281 cgctcgtcaa ggccctgacc ggcatcgaca ccgaccggtt gaaggaggag aaggagcggg
     2341 ggatctccat cgagctgggg ttcgccaacc tggccctgtc tgatgacctc cagctcggga
     2401 tcgtggacgt tccgggacac gaacggttcg tcaagacgat gcttgcgggc gtcggcggga
     2461 tcgacctggt ggtgctggtc atcagcgccg acgagggggt gatgccgcag acgcgggaac
     2521 accttcatat ctgcgagctg cttcaggtca agcggggcgt cgtagcgctg acaaaggccg
     2581 atctcgtcga gtccgattgg ctggagatgg tccaggccga tctgaaaggg ttcctggctg
     2641 gg
//
LOCUS       DATMXI010000170         3918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_71768, whole genome shotgun sequence.
ACCESSION   DATMXI010000170 DATMXI010000000
VERSION     DATMXI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71768"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..694
                     /locus_tag="VN444_03225"
                     /pseudo
     CDS             261..694
                     /locus_tag="VN444_03225"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
     gene            945..1169
                     /locus_tag="VN444_03230"
     CDS             945..1169
                     /locus_tag="VN444_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWR20857.1"
                     /translation="MPVSISLRLPEATAKALDELASATERSRTYLIRKALEAYMAEYA
                     DYQVALDRLRDKDDPVISSAELRKRLGRKN"
     gene            1153..1425
                     /locus_tag="VN444_03235"
     CDS             1153..1425
                     /locus_tag="VN444_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR20858.1"
                     /translation="MAARIEYKASVARDLRKLSHAAAVRVLLKVERMLASEGYQGEAL
                     SREFAGLYKLRVGDYRVIYARTEKGFLVLRIGHREDVYRKGPPEVN"
     gene            1839..>3918
                     /locus_tag="VN444_03240"
     CDS             1839..>3918
                     /locus_tag="VN444_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HWR20859.1"
                     /translation="MDRTEPNEVAEMSPERDKAIRLFTYLKDLCALRTTQVRNVATYD
                     QVFWLSDLPRHKLCRCTIWHLTDPSSPTSEQHSDLWIEIRKPALKSPPELPDELEPWI
                     KDEEITDSSLNEPGFYEQIPLSTLQDDSVNTDPNAFALINDYPHVFDKWVEYVEAKWK
                     PWASEDRELQKVQKAYNQLFNIYQRQEKLGEQYEVILGAGLLLWKSPNSGEIKRHLLA
                     IQSRIEFDRVRGIMSVGPALDGPQPMLECSMLETTDRPNPTDLTGIEKDAISLDGDPW
                     DAVVLECVLRGFANSLPIAGDYVPSLEHAGGASGKPIVRLAPALILRKRTRRTFEDFY
                     RQIIDQIKEGEEIPENIRRIIEIVDDSPSEGKEDVQIEAGTAPPALSDNELYFPLPAN
                     DEQKRIVHKVEHGRGVLVQGPPGTGKSHTIANLICHFLAKGKRILITSETPRALGVLR
                     SKLPSEIEELCVVWLGSGPDSQKALEKSVLGITQRKANWNASTELSLINQYVRRLDAE
                     RKEQARLRHDLRACRESDIYQHSSVFGRYSGTLQQIAVQIHEDRDRFQWFVDRPTDEI
                     DPVVTADELLRMAQIHRKLTPEIVALRKYRLFPLEHLIQPNEFSRLVEAEKKADLAHK
                     EAQDKRSYPGYAQLSALNREKRETVKRMIEGILATQAALSKHFHSWADRASREISGDQ
                     DRVWRQILAST"
BASE COUNT          944 a         1051 c         1096 g          827 t
ORIGIN      
        1 tgacagagcg accactgcga agggatgggt tcatcgggaa gtgagcaaaa aaattggacg
       61 aggaatacgc gcgttgcaga ggggaagacc aagaaaggat ctcaataaat agtgtctgtc
      121 cctataccct ataccggcgg gtatcgaagt atggcgtagg gtctgagaga cggtaagatc
      181 tagaaaaagg aggacgctgg atgaagcgct ggacgttcga acctggacat accgcagccg
      241 agttctgcgt cagacacatg atggtcacgg atgtccgggg gcacttcaag aacgtgcatg
      301 gcacgctcga gttcgatccg gagtttcccg ccttgtctcg cgtcgaggcg gtcatcgatg
      361 cgcggggcct ctggagcggt gagcgtgatc gcgataccca tctcaagggt cctgacttcc
      421 tgatgtagag cgctttcccg agattacctt tcgcggaagc caggttcgcc tccgcggggc
      481 caacgaggct gtcgttctcg gggatctcac tattcgtgga gtgacccgct ccatcgaact
      541 ccaagtccac tatctcgggc agtggcagac gccgtggcgc agagacgagt ccctccgatg
      601 ccgtgttcag tccgattttg ccaacattca atcgcgaccg accctcattc gctctttctt
      661 tcatgcgcca cgcctcgcct ccactaggga ctgagtagta agcgtgtcgg tcgggtggat
      721 ccttccaggc tcacccgacc gctttgtctg accagatagg gatcccgatc aagcccacca
      781 cggtataatc gggttcgtgc ttcctaccca ttgtctttcc agcgtggacc accagggact
      841 tcttagactc cgcgttcaac gagccgacct ccacttgaac cttgatctcc gtgcccgcta
      901 gccgtatact tgtcttgcat gtattacaca tgaggaggta cgcaatgccg gtgtctatct
      961 cactacgact tcctgaagcc actgcgaagg ccctcgacga gctggcgagc gctaccgagc
     1021 ggtcgaggac ctaccttatt cggaaggcgc tggaagccta catggcggag tacgctgact
     1081 accaggtggc gttggaccga ttgagggaca aggacgaccc tgtcatctcc tcggctgagc
     1141 tacggaagcg ccttggccgc aagaattgag tacaaagcct ccgtcgcgcg ggatctgcgg
     1201 aagctcagtc acgccgcagc ggtacgagtt ctgctgaaag tcgagcggat gctggcttcc
     1261 gagggctacc agggggaagc cctctccaga gaattcgcag gactgtacaa acttcgcgta
     1321 ggagactatc gcgtcatcta cgcccgaacg gagaaaggat tcctcgtgct ccggatcggt
     1381 caccgcgaag atgtctacag aaaagggcct cctgaggtca actaacgagc tattcgcggc
     1441 taagattgtt caccttcgat tcactggagg gtttggcgcg accacagttc cattaaaaag
     1501 gcagcgccgt ccaaaagctc ccataaagtg caagcacaag tcaacgctgg gtttcgtttt
     1561 ccgcttgacg ctgcttctca gagggtatta ctattcggcc acttcgatga ctcccgttca
     1621 atgtgaagcg aaggagcatg acgatggcag tcggtgaggc aacggctgtg agaagtcaga
     1681 ctcgcacgtc tggatggagc tgtaggtatg gcggcccttc agggcggttc aagccccgaa
     1741 gggccgttgt gtttcgaggc taccctcggc gttgtcgagt agaccgatcg aggggacaaa
     1801 agcggcccga agcagcgcgg ctgtggaatt gagatcttat ggatagaact gaaccaaacg
     1861 aagtcgcaga aatgtcgcct gagcgtgaca aggccatacg acttttcacg tatctaaagg
     1921 acctctgcgc tttgcgtacg acccaagtcc gcaacgtcgc gacgtacgat caggtcttct
     1981 ggcttagcga tcttccgcgc cacaagctgt gtcgttgtac aatctggcat ctgaccgacc
     2041 catcgtcacc gacatccgag cagcattccg atctatggat cgaaatccgc aaacccgcgc
     2101 tcaagagccc ccccgaactt cccgacgagt tagagccgtg gatcaaagat gaggagatta
     2161 ccgattcttc gttgaatgaa cctggcttct acgagcagat tccgctctcg acacttcaag
     2221 acgattccgt caatacagac cccaatgcct tcgcattgat taacgattat ccgcatgtct
     2281 ttgataagtg ggtcgagtac gttgaggcga agtggaaacc ttgggcgagc gaggatcgcg
     2341 agctacaaaa ggtccagaag gcttacaacc agctatttaa catttaccag cggcaagaga
     2401 aacttggcga acagtatgag gttattttag gggctggact cctcttgtgg aaatcgccga
     2461 acagcgggga gatcaagcgc catctccttg ccatccaatc gcgaatcgaa tttgacagag
     2521 tcagaggaat aatgagtgtt ggccctgcgc ttgatggtcc ccaaccgatg ctcgaatgta
     2581 gcatgctgga gacgacggat agaccgaacc caaccgacct cacgggcatc gaaaaggacg
     2641 caatctcact ggacggtgac ccgtgggacg ctgttgtact cgaatgcgtt ttgcgagggt
     2701 tcgccaattc gcttccgatt gctggggatt atgttccgtc attggaacat gccggtggag
     2761 catccggtaa acctatcgtc aggctcgccc ccgctttgat tcttcgcaag agaacacgac
     2821 ggaccttcga ggacttttac cgccagataa ttgaccagat taaggaaggg gaagaaatac
     2881 ccgagaatat tcggaggatc atcgagattg tcgatgactc acctagtgag ggaaaagagg
     2941 acgtccagat cgaagcaggc actgccccgc ctgcactgtc tgataatgaa ctgtacttcc
     3001 cgcttcctgc gaacgatgag cagaagcgga ttgtccataa agtcgagcat ggacgaggcg
     3061 tgcttgtcca aggtccgcca ggtaccggca agtcacacac aatcgcgaat ctcatctgtc
     3121 attttctggc aaaaggtaaa cgtatcctca tcaccagcga gacaccgcga gccttggggg
     3181 tattgcgcag caagttgccc agcgagattg aagagctttg cgtggtctgg ctagggtcag
     3241 ggcccgactc acaaaaggcg cttgagaaat cggtgcttgg aatcacacag cggaaagcaa
     3301 actggaatgc atcgacggaa ctcagcctga ttaaccagta cgtgcgccga cttgatgcag
     3361 agcgaaaaga acaggcgcga ctccgccatg atctgcgagc atgcagagag tcagacattt
     3421 accaacattc aagcgtgttt ggtcgctact cggggacgct tcaacagatt gcagtgcaga
     3481 tccacgagga tcgtgaccgg tttcagtggt tcgtcgatag accaacagat gagatcgatc
     3541 ccgtcgtcac ggcagatgaa ttgttgagga tggcgcagat acatcggaag ctgactcccg
     3601 agattgtggc cctacgcaag taccgccttt tcccgttaga acatctgatc cagcccaatg
     3661 agttttctcg tctcgttgag gccgagaaaa aggccgatct tgcgcacaaa gaggcacaag
     3721 acaagcgaag ctatcctgga tacgctcaat tatctgctct taatcgcgag aagcgggaga
     3781 cggtcaagag aatgatcgaa gggatcctcg caacgcaagc cgcgctctcc aagcatttcc
     3841 attcatgggc ggatcgtgcc tcccgcgaaa tttcaggaga tcaggatcgc gtctggagac
     3901 aaatcctcgc gtcaacga
//
LOCUS       DATMXI010000171         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_14714, whole genome shotgun sequence.
ACCESSION   DATMXI010000171 DATMXI010000000
VERSION     DATMXI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14714"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2138)
                     /locus_tag="VN444_03245"
     CDS             complement(<1..>2138)
                     /locus_tag="VN444_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWR20860.1"
                     /translation="HPTVILVDELAHTNAPDSRHTKRWQDVMELLGAGIHVYTTLNVQ
                     HLESLNDIVTKITGTVVRETIPDSVLEQADEIELIDLPPDDLLQRLKEGKIYVPELAK
                     EAIDNFFRKGNLTALRELALRRTADRVDAQMRAYMSDQAIPTTWPVTERLIVLVGPSP
                     HSAQTVRGAKRMAAALRAEWIAVYVETEAYARLSETDRRRVAENLRLAEQLGAEVVTL
                     SGSQTNESEAVLRYANERNVTKIILGKPTRPLWRRIVAGSIVDALVRGSGDIDIYVIS
                     GAGIPHAPVARVERVPKPDWSAYGRAATVVALCTAVAWLMNPYFELSNLIMVYLLGVT
                     GVAARSGPGPSILASFLSVAAFDFFFVAPHFTFRVSDAQYLVTFAVMLVVALVISGFT
                     VRIRIQAESARQRERRTAALYALSRELASARGVEHVLRAATRHIAEVFGGQVAVLLPD
                     PSGHLGLQVGPSAQFEVTPSERGVAQWVYEHGQTAGCGTSTLPGARVLYLPLVASQGI
                     LGVLGLLPAEPRSLEAPEQLHQLETFANQTALALERTQLAEAAQEAQIRAETERLRSS
                     LLSSVSHDLRTPLATITGAASSLLEGDKILDDQTQKDLLESLSEEAERLNRLVNNLLE
                     MTRMESGTLQVRKEWHVLEEVVGAALGRLAKLLRDRPVTTSLPANLPLVPIDDVLLEQ
                     VLINLLDNAVKHTPDGGPLEITAWAHDGAV"
BASE COUNT          383 a          688 c          687 g          380 t
ORIGIN      
        1 tgactgctcc gtcatgcgcc cacgccgtaa tctctagagg gcccccatca ggggtatgct
       61 tgacggcgtt gtccagcaga ttaatcagca cctgctcgag cagaacgtcg tcgatcggaa
      121 cgaggggcag attagccggc aacgacgtgg tcacggggcg gtcgcgcaag agcttcgcca
      181 agcggcccag cgcggcaccg acgacctcct ccagcacatg ccactccttg cgaacctgga
      241 gggtcccgga ctccatccgc gtcatctcga gcaggttgtt caccaggcgg ttgaggcgct
      301 cggcctcctc ggagagcgat tccagcagat ctttctgggt ctggtcgtcg aggattttgt
      361 ctccttccaa caggctgctc gcggcgccgg tgatggtggc gaggggcgtc ctgagatcgt
      421 gggacaccga actcaggagc gagctgcgca gccgctcagt ctccgcgcgt atctgggcct
      481 cttgcgcggc ctcagcaagc tgggtgcgct cgagtgccag tgcggtctga ttggcaaatg
      541 tctcgagctg atggagttgc tcaggcgctt cgagcgatcg cggctccgcc ggcagcaacc
      601 ccaacacgcc caggataccc tgcgacgcga ccaaaggcag atacaagacc ctcgctcccg
      661 gtaacgttga cgtcccacaa ccagccgtct gtccatgctc atacacccac tgggccaccc
      721 cacgctccga gggggtcacc tcaaactggg cggacgggcc tacctgaagg ccgaggtggc
      781 cgctcgggtc cgggagcagc accgcgacct ggccgccgaa gacttcagcg atgtgtcggg
      841 tggcggcccg caacacgtgt tctacgcctc gcgcgctcgc cagttcccga ctcagcgcgt
      901 acagcgcggc cgttcgccgc tcgcgctggc gagcggactc cgcctggatg cgaatccgca
      961 ccgtgaagcc gctgatcaca agggctacga cgagcatgac cgcaaacgtc acgaggtatt
     1021 gggcatcaga tacccgaaac gtaaagtgcg gggcgacgaa aaagaaatcg aaggctgcca
     1081 cactgaggaa agaggccagg atggagggac caggtccgga gcgggcggca acgccggtca
     1141 ccccgagcag atataccatg atgagattgg agagctcgaa atacgggttc atcagccatg
     1201 ccaccgccgt gcagagcgcg acaacggtcg cggcacggcc ataggctgac cagtccggct
     1261 tggggactcg ctccaccctg gcaaccggcg cgtgcgggat ccccgctccg ctgataacgt
     1321 agatatcgat gtcaccactg ccgcgcacaa gagcgtcaac gatggacccg gccacgatcc
     1381 gcctccacaa ggggcgggtc ggtttgccca gaatgatctt ggtgacgtta cgctcgttgg
     1441 cataccgcag gactgcctca ctctcattgg tctgcgaccc gctgagggtg accacctccg
     1501 ccccgagctg ctcggccagc cggagattct cggcgacgcg ccttcggtcc gtctcggaca
     1561 gccgggcgta cgcttccgtc tcgacataga ccgcgatcca ttccgcccga agcgctgccg
     1621 ccatccgttt ggcgcctcgc acggtctggg cagaatgtgg gcttgggccg accaagacta
     1681 tgaggcgctc cgtgacgggc caggtcgtgg ggatggcctg gtcactcata taggcgcgca
     1741 tctgcgcgtc cacgcggtcg gcagtgcgcc ggagcgccag ttcccgcagc gcggtcagat
     1801 tgcctttccg gaagaagttg tcgatcgcct ccttcgccag ctccgggacg tagatcttgc
     1861 cttccttgag acgctggagc aggtcgtcag ggggcaggtc gatcagctcg atctcatcgg
     1921 cctgttcgag gaccgagtcg ggaatcgttt cgcgcacgac tgtcccagtg atcttggtga
     1981 cgatgtcgtt gaggctctcg aggtgttgga cgttcagcgt ggtgtagacg tggatcccgg
     2041 cgccgagcag ttccatgacg tcctgccagc gcttcgtgtg tcggctgtcc ggcgcgttgg
     2101 tatgcgccag ctcatcgacc aggatcacgg tgggatgc
//
LOCUS       DATMXI010000172         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_138015, whole genome shotgun sequence.
ACCESSION   DATMXI010000172 DATMXI010000000
VERSION     DATMXI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138015"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VN444_03250"
     CDS             complement(<1..424)
                     /locus_tag="VN444_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HWR20861.1"
                     /translation="MKPALGKVAYINCEPVYYGIEQGAIPAECRIVEGTPAELNRMLQ
                     AGELDLSVISAIEYACHADRYLILPDLAIGSDGPAESVLLLSRVEPGDLDGEPVRLSR
                     DSLTSVFLVKLLLAKAFGVRPRFLPAGAETADSIPEDVA"
     gene            complement(421..1623)
                     /locus_tag="VN444_03255"
     CDS             complement(421..1623)
                     /locus_tag="VN444_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWR20862.1"
                     /translation="MIRDGAICAVGKASALIRDFAAEPHDDLGSAVLLPGLVNAHTHL
                     ELTGLHGRLPVGRSFTEWATALLNLRSELDGAFFAASARLGTATLLRSGVTCVADITT
                     SGRSVAPLKAAGLRGIIFQEILGLDPEQATERLDAAEEALRSLQLQASGSLLSVGLSP
                     HAPYSLSEPLLLRCAELLRRRRLPATIHVAESPEEVAYIGLGLGSIASELLPAVGRHS
                     PSHRVCGESPVAFLDRAGLLSEQLLAVHGVHVGGSDLELLKQRGVALAMCPRSNDYLN
                     VGTAPLPRYLASGLRVGLGTDSLASNETLSIWDEMQFAHRLYGGVVKAQQLVTMATLG
                     GATVLGMAGAIGSLTPGKRADLTALAIDHLDDTDPYGSLLRQASDGLVVLSMVEGKIV
                     YQREGAER"
     gene            complement(1699..>2257)
                     /locus_tag="VN444_03260"
     CDS             complement(1699..>2257)
                     /locus_tag="VN444_03260"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWR20863.1"
                     /translation="AYIEFGGKRLIEATVDCLRALFPEILIIANDPPLYAYLGVKVIP
                     DLIPDSGSLGGIYTGLSAASYPACFFVACDMPFLNADLIKLLIREAEGWDVVVPRVGG
                     ELQPLHAVYAKSCLPLIKEAIDGGVLKIARFFPKAKVKIIEEPAIRAVDPHLLGFMNV
                     NTPPELEQAEAIRRHPYRYQRSSDS"
BASE COUNT          470 a          725 c          675 g          387 t
ORIGIN      
        1 cagcaacatc ctccgggatc gagtcggccg tctctgcccc ggctggcaga aaccgaggcc
       61 tcaccccgaa tgccttcgcg agcaggagct tcaccagaaa gaccgaagtg agggaatccc
      121 tgctcaggcg gaccggttcc ccatcgaggt cgccgggctc cacccggctc aagagcagga
      181 cgctttcagc cgggccatcc gagccgatcg ccaggtccgg gaggatcaga tatctgtccg
      241 catgacacgc atactcaatg gcggagatga ctgacagatc gagttcccca gcctggagca
      301 tacggttcag ctcggcaggg gtcccctcaa cgatccggca ttctgccggg atcgcgccct
      361 gctcgatccc gtaatagacc ggctcacaat tgatatatgc taccttgccc agtgccggct
      421 tcaccgctcc gctccctcgc gttggtacac gatcttccct tccaccatac tcaagacgac
      481 caagccatca gaggcctggc ggagaagcga tccatacggg tcggtatcgt cgaggtgatc
      541 gatggccagg gcagtgagat ccgcccgctt accgggcgta agcgacccga tggcccctgc
      601 catcccgagt accgtagccc cgccgagcgt tgccattgtc acaagctgct gcgccttgac
      661 caccccgcca tagagccgat gcgcaaattg catctcgtcc caaatgctca gcgtctcgtt
      721 gctggccagt gaatcggttc ccaggccgac tcgaagtccg gacgccaggt accgggggag
      781 gggagcagtt cccacgttga gatagtcatt acttctggga cacatggcca gtgccacccc
      841 tcgctgtttc agcaactcga gatctgaccc ccccacatgc accccgtgga cggccagtaa
      901 ctgctccgag agcaggccgg ctcggtcgag aaacgcgacc gggctttcgc cgcagactcg
      961 atgggatggc gaatgccggc ccacggcggg tagcagctca ctagcgatcg acccgagccc
     1021 tagcccgata tacgccacct cctcgggaga ttcggccacg tgaattgtcg ccgggaggcg
     1081 ccgtcgccgc agcagttcgg cgcagcgcag cagcagtggc tctgacaggc tgtacggcgc
     1141 gtgtggtgac aggccgaccg acaacagact tccgcttgct tggagctgaa gtgaccgaag
     1201 agcctcctcg gccgcgtcga gccgttctgt ggcctgctca gggtcaagtc ccaggatctc
     1261 ctgaaagatg atacccctga gccccgctgc cttgagaggg gctacgcttc tgccggacgt
     1321 cgtaatatcg gcgacacagg tgacgccgct tcggagcagg gtcgcggtac ccaggcgagc
     1381 tgacgcggcg aagaacgcgc catccaactc ggagcggaga ttgagcaggg cggttgccca
     1441 ttcggtaaac gatctgccaa caggaaggcg gccgtgcagc ccggtcagct ccaggtgggt
     1501 gtgcgcattg accaagccgg ggaggagaac tgcactcccc aaatcatcgt gaggctccgc
     1561 cgcgaaatcc ctgatgagcg cggacgcctt acccactgcg cagatggccc cgtcgcggat
     1621 caagaggcca ccgtccaaaa tgggagggct ggaaatggga aacaaaaaac gagcggtcag
     1681 aatcacggca gccgcatttc agctatcaga tgatcgctga tacctgtatg ggtgtcgcct
     1741 gatcgcctcg gcctgctcaa gctccggtgg agtattgacg ttcataaacc cgaggagatg
     1801 agggtccacc gccctgatcg ccggctcctc gatgatcttc accttggcct tcgggaagaa
     1861 ccgggcaatc ttcagcacgc caccgtcgat ggcctcttta atgagcggga ggcacgactt
     1921 cgcgtacacg gcatgcagcg gctgcaactc acctcccaca cgtgggacca cgacgtccca
     1981 gccttcggcc tcgcggatca agagcttgat caggtcggcg ttaaggaaag gcatatcaca
     2041 ggcaacgaag aaacacgcag gataactcgc agcgctcagc ccggtataga tcccgccgag
     2101 cgagccggaa tcggggatca gatctgggat gaccttgaca ccgaggtacg catagagcgg
     2161 agggtcattg gcgataatca ggatctctgg gaagagcgct ctcaggcagt ccaccgtcgc
     2221 ctcgatcagc cgcttgcccc cgaattcgat ataggcc
//
LOCUS       DATMXI010000173         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_136114, whole genome shotgun sequence.
ACCESSION   DATMXI010000173 DATMXI010000000
VERSION     DATMXI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136114"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VN444_03265"
     CDS             complement(<1..231)
                     /locus_tag="VN444_03265"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWR20864.1"
                     /translation="MRFTAAAKVFVLFGDPVEHSLSPAMQNAALQTAGIDGLYIPWRV
                     KSADLPTAFESLRGMENFGGANVTLPHKEQAVS"
     gene            complement(403..>2017)
                     /locus_tag="VN444_03270"
     CDS             complement(403..>2017)
                     /locus_tag="VN444_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWR20865.1"
                     /translation="CIHVTPKNRGLFREDPVRLLKVFWYAQQMGYTLSQEIKDYIRAD
                     THLIDEGVRRSSRALGFLLAILREPKGVAATLRSMHELGVLGTYIPEFAKLTRLVQFD
                     FYHRYTVDEHTFLAVETLEHLDEISRFYGEEFRSIASDAKRLEILRLALLLHDIGKGE
                     GPDHVHKGASLVEASLVRMGIPEPDAEAIRFLVARHLEMSHIAQRLDLDDEAIVIDFA
                     KKVQTPDRLKMLYLLTYADIKAVGPGVWTEWKGTLLWELYIKTHTILIRGIPEGEDDL
                     ARAERVKSQLTQELSPEFGVEAVKRHLEEAPARYLLTTSLHRVASHMRLIQHVKRGEE
                     AASQWAAFPLAGYSEFTLCAYGRHGRFAQIVGTLTANGMNILSAQIFTLTSGMVIRHF
                     RVDSGGGAAIEDATVWDRAVADLRDVLAGGVAVRELIKSRRKAVLTQPIHKGALPPTK
                     VEFDNMVSEAYTVLDVRTRDRLGLLYLISSTLSDLGVDLRSAKIMTEAEQVVDVFYVT
                     NKDGSKLMDEGRREQIRLALERALSEGLN"
BASE COUNT          407 a          619 c          557 g          434 t
ORIGIN      
        1 cgaaacggcc tgctccttgt ggggaagcgt gacattcgcg ccgccgaagt tttccatccc
       61 gcgaagtgac tcgaacgcgg tcggcaagtc ggcgctcttc actcgccatg gaatatagag
      121 gccatcgatt ccggccgtct ggagtgcggc attttgcatg gccggtgaga ggctatgctc
      181 cacaggatca ccgaagagta cgaacacctt tgcggcggca gtaaacctca tttgtcgcta
      241 ctcaagtaga tagactgaag actgaaggcc tttaggggta gaaaccgaga agatcttgaa
      301 gggctcgact cgcgccttgc gagatattta tagtcttcag ccttcagctt cttcctgacc
      361 ttgctgatgg ctgatggctg accgctgaag gctgctttta ctttaattga gcccctccga
      421 gagggcccgc tccagtgcga gcctgatctg ctctctccgc ccctcatcca tcagcttgct
      481 cccgtcctta tttgtcacat agaagacgtc cacaacctgc tcggcctcgg tcatgatctt
      541 ggcggagcgc agatccacgc cgagatcaga gagcgtgctg gagatgagat acaggagtcc
      601 cagtctatcg cgtgtccgga catccagaac ggtgtaagcc tccgagacca tgttgtcaaa
      661 ctccactttg gttgggggca aggcgccctt gtggatcggc tgggtcagga ctgccttccg
      721 gcgactcttg atcagctcac ggacagccac cccgcccgcg agaacatccc gcaggtccgc
      781 tacggcccga tcccagacgg tcgcgtcctc tatggccgcg ccgcctccac tgtcgaccct
      841 gaagtgccgg atcaccatcc cgcttgtcag ggtgaaaatc tgagcactca gaatgttcat
      901 cccgtttgcg gtgagtgtcc ctacaatctg agcgaaccgc ccgtggcggc cataggcaca
      961 gagagtaaac tcggagtagc cggccaacgg aaatgccgcc cattggctgg ctgcctcttc
     1021 accccgtttc acgtgctgga tcaagcgcat gtgcgaggcg accctgtgaa gtgaagtcgt
     1081 gagcaggtac cgtgcaggcg cctcctccag gtgccgcttt accgcctcca cgccgaactc
     1141 tggtgacagt tcctgggtaa gctgggattt cacccgctcg gcacgagcca gatcatcctc
     1201 accctccggg atgcctctga tgaggatggt atgggtcttg atgtacagtt cccacagcag
     1261 cgtccctttc cattcagtcc agacaccggg cccaaccgcc ttgatgtcgg cgtaggtgag
     1321 caggtacagc atcttcagcc gatcgggtgt ctgcaccttt ttggcaaagt cgatcacgat
     1381 ggcctcatcg tcgaggtcca agcgctgggc gatgtgcgac atctccagat ggcgcgccac
     1441 taagaagcgg atggcttcag cgtccggctc cgggatgccc atccgcacca agctcgcctc
     1501 gactaaagac gcccccttgt gtacgtggtc gggcccttcc cctttgccga tgtcatggag
     1561 caagagcgcc aagcggagga tttccaatct cttcgcatca gatgcgatcg aacgaaactc
     1621 ttcgccgtaa aatcttgaga tctcatccag gtgttcgagg gtttcaacgg ccaggaaggt
     1681 gtgctcatcc accgtgtacc tatggtagaa atcgaactgg accagacgag tgagcttggc
     1741 gaactccggg atataggttc cgagcactcc aagttcatgc atgctccgaa gcgtcgcggc
     1801 cacccccttc ggttctcgaa ggatggccag caagaagccc agggcccggc tcgatcgcct
     1861 caccccctca tcgatgagat gcgtatcggc tctgatgtag tccttaatct cctggctcag
     1921 cgtgtatccc atttgctgcg cataccagaa gaccttcagc aaccggacag ggtcctcccg
     1981 aaacagaccc cgattcttcg gcgtgacatg gatgcag
//
LOCUS       DATMXI010000174         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_166947, whole genome shotgun sequence.
ACCESSION   DATMXI010000174 DATMXI010000000
VERSION     DATMXI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166947"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..746
                     /gene="ilvN"
                     /locus_tag="VN444_03275"
     CDS             159..746
                     /gene="ilvN"
                     /locus_tag="VN444_03275"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWR20866.1"
                     /translation="MSDKIRKTPKEVGARHIITLLVENRAGVLARIAALIAAKGYNID
                     SLTVGETMDPSISRMSLVVRGDDSVVEQAVKQLNRLIDVIRVTDLTGEEFVERELVLV
                     KVKAKPEAKAEVLRIADIFRAKVVDVAPASYMVELTGAEDKLNAFIELLRPYGIQEFA
                     RTGMTVMTRGSKILSKQTEETVKGPDTKKVVGLAE"
     gene            769..1773
                     /gene="ilvC"
                     /locus_tag="VN444_03280"
     CDS             769..1773
                     /gene="ilvC"
                     /locus_tag="VN444_03280"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWR20867.1"
                     /translation="MTKLYYDQDADLNLLKGKAIAIMGYGSQGHAHALNFKDSGLDVI
                     VGLYRESKSWDKVKSDGLKVATCEEAALSADVIMMLLPDQTQRQVYQESIERALTPGK
                     TLMFAHGFNIHFHQILPPPTVDVSMIAPKAPGHLVRQVFTEGGGVPALLAIHQDVSGN
                     AKAMALAYAKGIGCTRAGVLETTFREETETDLFGEQAVLCGGASALVKAGFETLVNAG
                     YQPELAFFECMHELKLIVDLMYQGGLAYMRYSISDTAEYGDYSRGPRVVDERVKAEMR
                     KILEEIQSGAFAREWILENQAGRPSFLAIRKQEAEHPIEKVGKDLRSMMTWIKGPGTH
                     "
     gene            2026..2898
                     /gene="metF"
                     /locus_tag="VN444_03285"
     CDS             2026..2898
                     /gene="metF"
                     /locus_tag="VN444_03285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HWR20868.1"
                     /translation="MKIVDVYGTGKVGLSFEIFPPKTEAGECLLFAALEALMAYRPSF
                     VSCTYGAVGSTRENTLELTAKISKTFGVTTAAHRTCVGSTVEEILRWLEEAADLGIEN
                     IVALRGDPPKGQAEFTKPEGGFAHANELVTLIRQKFRHFGIAVAGYPETHQEAPSPAV
                     DLANLGRKVKAGADAVITQLFYDNRDFFDFRRRYEEARIAAPLIPGILPVVNLGQVQR
                     ITSMCGAKMPAPFLAELEAYRDDPDGQVGVGVRYAIRQCRELLDAGIPGLHFYLLNKS
                     EATFQILQALKLSG"
     gene            2965..3909
                     /gene="miaA"
                     /locus_tag="VN444_03290"
     CDS             2965..3909
                     /gene="miaA"
                     /locus_tag="VN444_03290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWR20869.1"
                     /translation="MPLIVLVGPTAVGKSSMALAVAERVGGEIIAADSMQVYRGLDIG
                     TAKPSADEQGRIPHHLLDLVDPDQPFTAADYARLASAAIADIRARGRLPILVGGTGLY
                     LRALFRGLFSGPWEMTPLRETLYQEAERVGSATLHQRLETSDPEAAAAIHPNDLFRIV
                     RALEVAAVSGRPISTLRAEARRNHRPILGPVLQFGLERNRHELYQRIEARVEAMMVQG
                     LLREVQGLLDRGYSPALRPLRAIGYRHMIGHLKGHIGLNDTVASLKRDTRRYAKRQLT
                     WFRHEDEIEWLFVEGSTLSERVLRLLVERIETAWSRTV"
     gene            3891..>4798
                     /locus_tag="VN444_03295"
     CDS             3891..>4798
                     /locus_tag="VN444_03295"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWR20870.1"
                     /translation="MVKNSVGRMRVNPVGPLKGELTLPGDKSITHRAIILGSIADGVT
                     EIAGALRSDDCRCTAKALGTMGATVEELSDDRLRIRGCGLQGLKEPDTILDVGNSGTT
                     MRLLAGVLAAQPFFSVLTGDQYLRARPMARVAMPLRSMGATILGREGGNFPPLAIMGT
                     HLKAIDYVSPIASAQVKSAILLAGLFTEGETTVTEPSLSRDHTERMFEAVGIPIDRNG
                     LSLRVGSVKRIPSFQVAIPGDFSAAAFFIVAALVIPGSELTLRDVGINPTRTGLLDAL
                     QSMGAVIEVSRPRIISGEPVADLHVR"
BASE COUNT         1023 a         1262 c         1523 g          990 t
ORIGIN      
        1 gtcctggaga aggcctttgc tatgccagga ccggttattg tggacatcgt cacagagcgt
       61 gaagaaaatt gctttcctat gattccacca ggtggggcga ttaaggatat catcgactac
      121 ggtgatccga ttcctgagaa gctgtttcag ggtttgagat gagcgataaa atcaggaaga
      181 caccgaaaga ggtcggggcg cgtcatatca tcaccctgct cgtcgagaac cgtgcaggag
      241 tcctggctcg cattgccgct cttattgccg ccaaagggta caacatcgac agcctcaccg
      301 tgggtgagac catggatcca tccatctccc ggatgagcct tgtggtgagg ggggacgact
      361 cggtggtgga acaagcggtc aagcagctca accggctcat tgacgtgatc cgtgttactg
      421 acctgaccgg tgaggagttc gtcgaacggg agcttgtgct tgtgaaggtg aaagccaaac
      481 cggaggcaaa ggctgaggtc ctccgaatcg ccgatatctt tcgggctaag gtggtagatg
      541 tggctcccgc atcgtacatg gtggaactga ccggcgccga ggataagttg aacgccttta
      601 ttgaactcct caggccgtat ggcattcagg agttcgcgag aacaggcatg acggttatga
      661 ccagaggcag caagatactc agcaaacaga cagaagagac ggtgaagggg ccggacacca
      721 agaaggtggt tgggctcgcc gagtagggga gaggggtgtg gaggggatat gaccaagctg
      781 tactacgacc aggatgctga tctgaatctg ctaaaaggga aggccattgc catcatgggg
      841 tatggaagtc agggccatgc ccatgcgctc aattttaaag atagtggcct cgatgtgatc
      901 gtcggtctct accgggagag caaatcgtgg gacaaggtca agtctgatgg gctgaaggtc
      961 gccacctgcg aggaagccgc cttaagcgcc gatgtcatca tgatgctgct gcctgaccaa
     1021 acccagcgac aggtctacca ggagtcgatc gaaagggcgc tcacgccagg gaagaccctg
     1081 atgtttgccc atggcttcaa catccacttc catcaaatcc tccctccacc aactgtcgat
     1141 gtgtcgatga ttgcgcccaa ggcgccggga catcttgtgc gccaggtctt tacagaaggg
     1201 ggcggcgttc cggcgctgct ggctatccac caggatgtgt cggggaacgc gaaggcaatg
     1261 gccttggcct atgccaaagg gatcggatgt acgagggccg gcgtgctcga gacaaccttt
     1321 cgcgaggaga ctgagactga cctgttcggt gaacaggccg tcttgtgcgg aggggcctcc
     1381 gccctggtca aggccggatt tgagacgctg gtgaatgccg gctaccagcc tgagttggcc
     1441 ttcttcgaat gcatgcatga gctgaagctg atcgttgacc tcatgtacca gggaggcttg
     1501 gcgtacatgc gctattcgat cagcgatacg gcagaatacg gggactatag ccgcggtccc
     1561 cgcgttgttg acgagcgtgt caaggccgag atgagaaaga tccttgagga gatccagagt
     1621 ggcgcctttg ccagagagtg gatcctcgaa aaccaggccg gccggccaag cttcctggcg
     1681 atacgcaagc aggaggcaga gcatccgatc gagaaggttg gtaaggatct gcggtccatg
     1741 atgacctgga tcaagggacc ggggacgcat taagcagcag tctcacgggg aagaagcctt
     1801 cagctatcag cgatcagcgg tcagcaagac tgggggctga cggctaatcg ctgtatactt
     1861 acgcatacga caggccctcg cgacgggtgg acagaggcga gggcctttgt ctatggcgcc
     1921 ccatggcaat taagggccct cccgcatggt gttgggtgct gttgcatttt tgctcgcgta
     1981 ctcgcttgaa ggcagacggt ggtagaggag tagtggctga gacgaatgaa gatcgtcgac
     2041 gtatacggca caggtaaggt tggactttcc ttcgagatat tccctcccaa gactgaggcc
     2101 ggggagtgcc tgttgttcgc cgccctcgag gccctgatgg cgtaccgtcc ctcctttgtg
     2161 tcatgcacct atggcgccgt aggatcgact cgggaaaata ccctggagct gactgccaag
     2221 atcagcaaga catttggcgt gaccacagca gctcaccgaa cctgcgtggg atcgaccgtg
     2281 gaggagattc tgcggtggtt ggaggaggct gcagatctcg gcattgagaa catcgtcgcc
     2341 ttgcgcggtg atcctcctaa ggggcaggca gagtttacaa agccggaggg tgggtttgcg
     2401 catgccaatg aactggtgac gctgattcgg caaaagtttc gtcactttgg tattgccgtt
     2461 gccggctatc ccgaaacgca tcaggaggcg ccaagccctg cggttgattt ggcgaatctg
     2521 gggcggaagg tcaaagcggg agccgacgcc gtgatcacgc agctctttta tgacaatcgc
     2581 gatttcttcg actttcgcag gcgttacgaa gaggccagga ttgcagcgcc tctgattcca
     2641 ggtatcctgc cggtcgtcaa cttgggccag gttcaacgta ttacatctat gtgtggggct
     2701 aaaatgccgg ccccgtttct tgccgaactg gaagcgtatc gggatgatcc tgatggacag
     2761 gtgggtgtgg gggtgcggta cgccatccgg caatgtcgcg aactgcttga cgcaggtatc
     2821 cccggcctgc acttttacct tctcaataaa tctgaagcca ccttccagat cttgcaggca
     2881 ttgaagctgt ccgggtaaca ggcagataga ctgaaacctg aaggctttta ggggtaaaaa
     2941 ggaggcagca atctcttgag atcggtgccg ctcattgtgc tggtgggtcc gactgcagtc
     3001 gggaagtcat ccatggctct cgccgtagcg gagagggtag ggggcgagat catcgcggcc
     3061 gactcgatgc aggtctaccg cggccttgac atcgggacag caaagccgag cgctgacgaa
     3121 caagggcgca tcccgcacca tctcctggac ttggtggacc ctgatcagcc attcacggca
     3181 gccgattatg cgaggctggc ttctgctgcc attgccgata tccgcgctcg tggacgtctc
     3241 ccgatcctgg taggaggaac cggcctctat ctccgtgcgc tctttcgtgg cctgtttagc
     3301 ggaccatggg agatgacccc actcagagag acactgtacc aggaggccga acgggtgggt
     3361 agcgcaaccc ttcaccagcg actcgaaacc agcgaccccg aggcggctgc ggcaatccat
     3421 cccaacgacc tctttcggat cgtgcgggcg cttgaggtgg cagccgtaag tggccgtccc
     3481 atctctacgc ttcgggcaga ggcacgtcgc aaccacagac cgatcctggg gcccgtgctg
     3541 cagtttgggc ttgagcggaa tcgtcatgag ctgtatcagc ggattgaggc gagggtagag
     3601 gcgatgatgg tccagggatt gttgcgtgag gtccagggtc tgctcgatcg gggctacagc
     3661 ccggcactgc ggccgctacg agccatcggg tatcgtcata tgatcgggca tctgaaagga
     3721 cacatcggcc ttaatgacac ggtcgcgtct ctcaagcgtg atacacgacg gtatgccaaa
     3781 cgtcagctca catggttccg tcacgaagac gagatcgagt ggctttttgt agaaggatcg
     3841 acgctgagcg agcgcgtgct tcgcttgctc gtcgagcgaa tcgagaccgc atggtcaaga
     3901 acagtgtagg gcggatgcgg gtgaaccctg tcggcccgct gaagggtgaa ctgacccttc
     3961 caggcgacaa gtcgatcacc catcgcgcga tcatcctggg ctcgatagct gatggagtca
     4021 ccgagattgc cggcgcgctt cgaagcgacg actgccgttg caccgctaag gccttgggca
     4081 caatgggcgc aacggttgaa gagttgagtg acgatcgact gcggattcga ggatgcgggc
     4141 tgcagggcct gaaggaaccg gacacgatcc tggacgtcgg gaattccgga acaaccatga
     4201 ggctcctggc gggcgtactg gcggcgcagc cattcttttc cgtgctgact ggcgaccagt
     4261 atctgcgtgc gcggccgatg gccagggttg ccatgcccct gaggtcgatg ggggcaacta
     4321 tcctgggtcg tgagggcggc aacttccctc ccctcgccat catgggaaca cacctgaagg
     4381 cgatcgacta tgtcagtccg atcgccagcg cccaggtcaa atctgcgatc ctgctggccg
     4441 gtctctttac cgaaggggag acgacagtca cagagccatc tctctctcgc gatcatacag
     4501 aacggatgtt cgaggcagtt ggcataccca tcgaccgcaa cggcttgagc cttcgagtag
     4561 gtagcgtcaa gaggatcccg tcgtttcagg tcgccattcc gggcgatttt tcagcggcgg
     4621 cattctttat tgtagcggct ctggtgattc cagggtcaga actgacattg cgggatgtgg
     4681 gtatcaatcc gaccagaacc ggcctgttag atgcactaca gtcgatgggt gcggtgatcg
     4741 aggtaagccg accgcgtatc atctctggtg agccggttgc cgacctccat gtcaggag
//
LOCUS       DATMXI010000175         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_306833, whole genome shotgun sequence.
ACCESSION   DATMXI010000175 DATMXI010000000
VERSION     DATMXI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306833"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..3675
                     /gene="gltB"
                     /locus_tag="VN444_03300"
                     /pseudo
     CDS             1..3675
                     /gene="gltB"
                     /locus_tag="VN444_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809670.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
BASE COUNT          719 a         1144 c         1141 g          671 t
ORIGIN      
        1 gctggtcttt ctgcctactg acccttcgca ggccgcaaaa tgccaggcga tcttcgagga
       61 gctgatcctg gaagaggggc agacgctctt ggggtggcgg gacgtgccga ccgacgactc
      121 gttgatcggc cccagcgcca aggccgccga gccgaccttc aagcagatct tcattggccg
      181 taaccccgcc atccaggacg acctggcgtt cgaacggaag ctctacgtga tccgcaagca
      241 ggtcgagcat gtggtctacg gctcggccct gccgcagagg cagctcttct atctgccaag
      301 cctctcctca aatacgctga tctacaaggg gatgctttcg gcggatcaga tcgagacgat
      361 cttccaggac ctcatggacc cggctgtcga atcggcgctg gcgctggttc accagcgctt
      421 ctccaccaac accttcccct cctggccgtt ggcccacccg tatcggtata tggcgcataa
      481 tggcgagatc aacacgctac gcggcaacat caactggatg cgcgcccgcg aggcgctgtg
      541 cgaatccgag ctactgccag acctcaagag gatctttccg atcgtgctgg agggtgggag
      601 cgactcggcg atcttcgaca acgtcctgga gttcctggtc atggccgggc gttccctgcc
      661 ccacgccgtc ctcatgatga tcccggaggc ctggagcggt catgaatcga tgagcgagga
      721 acgcaaggcc ttctacgagt accatagttg cctgatggag ccatgggacg ggccggcctc
      781 gattgccttt accgacggga cggtgatcgg agcggtgctg gaccgtaacg ggttacgccc
      841 gtcgcgctac tacgtcacga aggacggcct ggtggtcatg gcctccgagg tgggggtgct
      901 ggacatcccg cccgagaatg tgctgattaa agagcgcctg cacccaggca ggatctttct
      961 ggtggacacc gcccagggga ggatcatcga cgatactgag ctgaagcgcg ccgttgctac
     1021 cgagcacccg tatcgggagt ggcttgaggc gaacctggtc ccgttggagg agctgccggc
     1081 gccctcgcac gtccatgagc cggatcacga gaccgtactg cagcggtagc agctcttcgg
     1141 ctacacccac gaagacctgc gcctcctgat aggaccaatg gccttgaagg gtgaggagcc
     1201 ggtcggatct atggggaccg atacgccgct cgccgtcctc tccaaccgtc cgcgtttgct
     1261 ctatgattat ttccagcaac tctttgccca ggtgaccaac cccccgctgg acgcgatccg
     1321 ggaggagttg gtgactcaaa tggcgacttc catcgggccg gaacggaacc tgctcaaacc
     1381 ggagcctgaa agctgccggc agatcaaatt gaagacgccg gtcctcgaca acgaggagtt
     1441 ggcgcgcatc cgctatatga atctacccgg cttcaggtcg atcacgctgc cgatgctgtt
     1501 ccaggcagtc gaaggtggca agggtctcga tcatgcgcta gaggagctgt gccttcaggc
     1561 gagccaggct attgccgatg gctacacctt cctcattctg tccgaccgtg gtgtgtgtaa
     1621 ggaactggcc ccgatcccgg ccctcctcgc caccgccggc gtccatcatc acctggtccg
     1681 ggaaggaacc cgcacgcggg tcgggctggt gatcgagagc ggcgagccgc gcgaagtcca
     1741 ccatgcggcg ttgctcatcg ggtacggcgc gggcgctatc aacccgtacc tggccttcga
     1801 gaccctcgac gacatgatcc gcgaggggct gcttccggag atggaccata agaaggccgt
     1861 caagcactac atcaaggcgc tcaacaaggg cgtcctgaag gtgatctcca agatggggat
     1921 ctccacaatc caatcctacc gcggcgccca gatcttcgag gcgatcgggc tggataaggc
     1981 gttcgtggac cggtgcttca cctggaccgc ttcccggatc ggcggcatcg gtatagacgg
     2041 gattgccgaa gaggtgatcc tccgccatcg caaggccttc cctgaccgac ccgtcggcca
     2101 ggctgacctt gagtggggcg gtgaatacca gtggcggcga gacggtgagt accatatcct
     2161 taaccctgat acggtcttca agctgcagca ctccacacgg accggccaat acacggtctt
     2221 caaagagtac tcagagctgg tgaacaatca tagccagagc ctgtgtaccc tccgggggct
     2281 gttcgatctc aagcgcgccg agcagccgat cccgatcgag gaggttgagc ccgtcgaatc
     2341 tatcctcaag cgcttcgcta cgggagccat gtcgtatggc tctatcagcc aggaggccca
     2401 cgagacgctg gccattgcca tgaaccggtt gggcgccagg tcgaataccg gagaaggggg
     2461 cgaggatccg gcccgcttca cgccggaccc caacggcgac tggcgccgca gcgccatcaa
     2521 gcaagtggcc tccggccgct tcggagtcac tagcgaatac ctggtcaatg ccaccgacct
     2581 gcagatcaag atggcgcagg gctctaagcc tggtgagggc ggccagctcc ccgggacaaa
     2641 agtctatccc tggatcgccg cggtgcggca ttcgactcct ggcgtgggcc tcatctcgcc
     2701 accaccgcac cacgacatct actctatcga agacatcaag cagctcatcc atgacctgaa
     2761 gaacagtaat ccacgtgcgc gtattcacgt gaagcttgtc tctgaagtcg gggtcgggac
     2821 gatcgcggcc ggggtggcca aggccttctc tgatgtggtc ctgatctcag gccacgatgg
     2881 cggtaccgga gcctcctcac tgagttccat caagcacgcc ggcctgccat gggaactcgg
     2941 gctagccgag acccagcagg tactggtgat gaacaggctc cgggatcgga tcagcgtcca
     3001 ggtcgatggt cagatgaaga cagggcgaga cgtcgttatc gccgcgcggc tgggcgcaga
     3061 ggagtatggc ttctccacgg caccgctggt tgtatcgggc tgcatcatga tgcgcgtctg
     3121 ccacctgaac acctgcccgg ttggggtcgc cactcaggat cccgaactca gaaagaactt
     3181 ctccggcaag cccgagtatg tggaaaactt cttccgcttc gtcgcccagg aggtacgcga
     3241 gctgatggcg gaacttggct ttcgcaccat ggatgagatg atcggccgtg tggataggct
     3301 cgatatgaag aaggcggtag agcactggaa ggccaagggg ctggactact cgtcgatcct
     3361 ctatcgcccg caggtcgggc cagaggtggc catccgtaag gtgcgcgaac aggaccacgg
     3421 cctggaacag tcgctggaca tgaccactat cgtcccgctc tgccagcccg cgctcgagcg
     3481 gcgtgagccg gtgggcctgc gcctgccgat caggaacgtc aaccgcacgg tcggcaccat
     3541 tctgggctcc ctggtgacca gccgctacgg aggcgacggc ctgccggagg atacgatccg
     3601 gatccacttc acagggtcgg ccgggcaaag tttcggcgcc ttcatccctc aagggatcac
     3661 gctggccctg gaggg
//
LOCUS       DATMXI010000176         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_249088, whole genome shotgun sequence.
ACCESSION   DATMXI010000176 DATMXI010000000
VERSION     DATMXI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249088"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1587)
                     /gene="rmuC"
                     /locus_tag="VN444_03305"
     CDS             complement(199..1587)
                     /gene="rmuC"
                     /locus_tag="VN444_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWR20871.1"
                     /translation="MTEWLTLLLGLWIGAVLGWAISIARAKAQVGAILREHEARTAAA
                     EARVEEVRQQLASARHDFDILRENLRQAETARTAAETRVVEIEKNLTEQKALLEDAKT
                     RLSDTFRSLAAEALAGNNAGFLILAEEKFKALKDEAAVDLEGRRKAIETMIQPLGETL
                     VAYQQETKALEDKRLREYSTVGEQLRAVAMGQTTLQNETAKLVNALRSPQVRGRWGEI
                     ALRKTAELAGMSPHCDFVEQESVTTGEGRFRPDMVVKLPAGREVVVDSKVPLGGFLEA
                     LEAKTDEDREMALLKHAAQVNQHVTKLASKEYWDQFAAAPEFVVLFIPNDSFLAAAAE
                     KDPVLVESALSKKVVIATPATFIALLRAIAYGWRQELLTENAQRISILGQELADRMAT
                     LAEHLIRVGGAIGKAVDSYNAAVASFESRVFPTARKFQVLGAGGKKEIQELQPIDQKP
                     RALNTFDIDDAE"
     gene            complement(1647..>2128)
                     /locus_tag="VN444_03310"
     CDS             complement(1647..>2128)
                     /locus_tag="VN444_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWR20872.1"
                     /translation="STVYDVAHNIVKVEEHEVDGRRMKLAVHRKGATRAFPPGHPELP
                     AHYQAIGQPVLIPGDMGRASFVLVGTGAMEQTFGSTCHGAGRVMSRHAAIRAAKGRAI
                     QRELEDQGIIVKASGGESLAEEMPEAYKDATQVVTVVHRAGLSKMVARLRPMGVIKG"
BASE COUNT          388 a          684 c          575 g          481 t
ORIGIN      
        1 ggcgacggtt ccagccgccc tctcagcctg ctcccgcaga ctcccgtact tgcttcctat
       61 catgttccaa tctagcctca atcaagaaag gtgtcaagaa tccctcagaa gcaggtctat
      121 ctgctctatc tggtctgttt ggtcctttgg tctgtgacgc gataggcgaa atagaccaaa
      181 tagacgaaat ggccagattt attctgcgtc gtcgatgtcg aatgtattca aggcgcgggg
      241 cttctgatcg atcggttgta gctcctggat ctccttcttg ccaccggctc cgagcacctg
      301 aaactttcgg gccgtcggga agacgcgact ctcaaacgag gctaccgcgg cgttatacga
      361 atccacggcc ttgccgatgg ccccgccaac cctgataaga tgctcggcaa gcgtcgccat
      421 ccgatcggcg agttcctggc ccaggatgct gatgcgctgg gcattctcgg taagcagttc
      481 ctgtcgccat ccataggcaa tggcccgcag taaggcgata aaggtggcgg gcgtcgcgat
      541 gacgaccttc ttcgagaggg ccgattcaac cagtaccggg tccttctcag cggccgcggc
      601 aagaaaggag tcgttcggaa taaagagcac aacaaactcc ggagctgccg cgaactggtc
      661 ccagtactcc ttcgaggcca acttcgtcac gtgctggttg acctgagccg catgcttgag
      721 gagggccatt tcacgatcct cgtccgtttt tgcttcgagc gcctcaagaa atccgccgag
      781 ggggaccttc gagtcaacca ctacctcgcg cccggccggc agcttgacga ccatatcggg
      841 acggaagcgt ccctcgccgg ttgtcacgct ctcctgttcc acaaaatcgc agtggggtga
      901 cattccggca agttccgctg ttttccgcag cgcaatctcc ccccatcgac cgcgaacctg
      961 tggagacctc agtgcgttga ccaacttggc tgtttcgttc tgaagtgtcg tctgacccat
     1021 cgcgactgcg cgcagttgtt cgccgaccgt actgtactcc ctgagccgct tatcttcgag
     1081 tgccttggtt tcctgctgat aggccacaag ggtttcgccg agcggctgaa tcattgtctc
     1141 gatcgctttt ctgcgaccct ccaaatcaac agccgcctca tccttcagcg ccttgaattt
     1201 ctcctcagcc aggatgagga atcccgcgtt gttgccggcc agcgcttcgg ccgccagcga
     1261 tctgaaggta tcagataatc tggtcttcgc atcttccagg agggccttct gttcagtcag
     1321 gttcttttcg atctcgacta ctcgggtttc agcggcagtt ctggcggttt cagcctgtcg
     1381 taaattctcg cggaggatat cgaagtcatg tcttgccgag gcaagctgct gtcgcacctc
     1441 ctccacccgc gcctctgcag cggccgttcg cgcctcgtgc tctcggagga tggcacccac
     1501 ttgagccttg gcccgggcta tactgatcgc ccatccgagg acggctccaa tccagagtcc
     1561 caacaacagg gtcagccatt cggtcatttc gagttccttc gaaaatagcg ttcagcgatc
     1621 agcattcagc cgccagcttc ttaccctcac cccttgatca cacccatggg gcggaggcgg
     1681 gcgaccatct tggagaggcc ggcgcggtgc acaaccgtca cgacctgcgt agcatctttg
     1741 tacgcctcgg gcatctcctc cgccaacgat tcgccgccgg aggccttgac gatgatcccc
     1801 tggtcctcca gctcccgttg gatggcccga cccttagccg ctctgatggc cgcatgtcga
     1861 ctcatgaccc gcccggcgcc gtgacacgtg ctcccgaaag tctgctccat agcgcccgtt
     1921 cccaccagta caaatgaggc gcggcccatg tcgcccggga tcagcacagg ttgaccgatg
     1981 gcctggtagt gagccggtag ctccggatgc ccgggtggga aggctcgggt cgctcctttg
     2041 cgatgaaccg ccagcttcat ccgtctgccg tcaacctcgt gctcctctac cttgacgatg
     2101 ttgtgggcca catcgtagac cgttgaca
//
LOCUS       DATMXI010000177         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_194322, whole genome shotgun sequence.
ACCESSION   DATMXI010000177 DATMXI010000000
VERSION     DATMXI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194322"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VN444_03315"
     CDS             complement(<1..1739)
                     /locus_tag="VN444_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWR20873.1"
                     /translation="MTKFRTKVLWSIIPMILALCVLFGGMSLYQHNRLLHREFVKHGK
                     ALASNLAASGELGVFSEDERFLSASLRGITGEEDVAYVFIYNDAGTLLIGGGRALTQP
                     GPTLTADTLSNEIRTQILATRQPVSRTLKGAGGESFLEFYAPILSAEVRLIEEQFFGM
                     PGSNRGPSENRTRVIGIARVGLSMRNIDVHSAYLIKLWAMLSIVFLAAGALAAYALSR
                     RITHPITRLTESAARMAQGQIDQEIPVDSRDEIGTLATTYNEMAKALKRTLDERTKVA
                     TELRDLNRNLEDRIRERTSQLRDLHQLGVSMQEPLPLSARLDLILKGVHEVIGFDRII
                     IWLPDREGLSLEMAAAVGFSSASGTVQVPISDEVPTLARAYLDRVEIIIEERHEVPPE
                     LRVHPPHDKVPALRSRSFVVLPLISRRRSVGVLAADNAVSRKPIAPQLDLLRIFASQA
                     AVAIENAQLFQEVEETNRQLARASRHKSEFLANMSHELRTPLNAILGFTELIIDEVYG
                     KVPDELREPIEDIHINGRHLLRLISDVLDLSKIEAGHMQLNLGEYSVQSFIDSVISAT
                     RSLAAEKRLELVGR"
     gene            complement(1792..2739)
                     /locus_tag="VN444_03320"
     CDS             complement(1792..2739)
                     /locus_tag="VN444_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR20874.1"
                     /translation="MSLLTTRTASLIFAACVIVGAIIHSSTPAEAAKIIALKSADVDV
                     YDEAMEGFKSVLRESTIVEYDMEGDLQKGKKFLARLKSGPKPDLILAVGIWALQVVLE
                     EIQDIPVVFAMVLNPTAVIRQEVRNVTGASMNVPNEQQLALLKKVSPQVRRIGVIYDP
                     SQTGFLVRQAERIAKDQGLRLVTKAITSPKDSFATLDAMQDEIDALWILPDLTVLAPE
                     SVQYMLLFSFRHKIPVLGLSENQARMGTLLSLSFESGWDIGSQAAELANGILSGRSVE
                     EIPFTTARKVRLTVNLKTAGKLGVHIPKELLDRAVVVIR"
     gene            complement(2758..>3423)
                     /locus_tag="VN444_03325"
     CDS             complement(2758..>3423)
                     /locus_tag="VN444_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWR20875.1"
                     /translation="GLVVPLIHVRLFARASADIFHVDTLGVTKPGTTQIGNLPIHQFG
                     PNSTPPDASGGGEETGLGDIILRTKYNFLRNSGGLTPDLAFLFEVKLPTGDAKRLLGT
                     GGTNLAGLLIASKTYGRWLTPHVNVGYEINTKELEQNTIRYALGFDARLLSGLSFDAD
                     VIGRQKPEGNGTGDHTVDLSLGVKWNPLNSLIFRANVQIPLDKNSGLRADFIPTIGVE
                     YLF"
BASE COUNT          756 a          946 c          952 g          769 t
ORIGIN      
        1 cggccgacca actccagccg cttttctgct gcgagggatc gcgttgctga gatcacagag
       61 tcgataaatg actgaacaga gtattcaccc agattcaact gcatgtgtcc ggcttcgatt
      121 ttcgagagat ccaacacatc gctgatgagc ctgagcaaat gccgaccgtt tatgtggata
      181 tcttcgatcg gctcgcgcaa ctcgtctgga accttgccgt agacctcatc gatgatcaac
      241 tcggtaaatc ccaggatcgc gttcaggggc gtccgtaact catgactcat gttggccagg
      301 aattctgatt tgtggcgact cgcccgcgcg agctgacggt tcgtctcttc tacctcctgg
      361 aagagctggg cgttctctat agctactgcc gcctgagagg cgaagatacg gagcaggtcg
      421 agctgcggcg caatcggctt tcggctcacc gcgttgtcgg cggcaaggac tccaacagaa
      481 cgtcttcgag agatcagcgg taagaccaca aagctgcgag agcgcaacgc cggcacctta
      541 tcgtgcgggg gatgcactcg aagctccggc ggaacctcat gcctctcctc aatgatgatc
      601 tcgactcgat ccagataggc ccgcgctaac gtaggcacct cgtcggagat tggcacctga
      661 actgtacctg atgctgatga aaaaccgaca gcggcagcca tctcaagaga aagaccctcg
      721 cgatccggta gccagataat aattcgatca aacccaataa cttcatgcac ccccttcaga
      781 attaagtcca gccgtgctga cagggggagt ggttcctgca tagatacgcc gagctggtgc
      841 aggtcgcgta gttgagatgt ccgctcgcga atccgatctt ccaggttccg gttcaagtct
      901 cggagctctg tggctacctt cgtccgctca tcgagggttc gcttcaacgc cttggccatc
      961 tcattgtacg ttgttgctag tgtcccgatc tcatcccggg aatcgaccgg gatttcctga
     1021 tcgatctgcc cctgggccat tcgtgcggca gattctgtca atcgggtaat cggatgcgtg
     1081 atcctccggg agagagcgta tgcggcgagg gcgccggcag ccaaaaagac aatagacagc
     1141 atggcccaga gtttgatcaa gtaggctgag tggacatcga tgtttcgcat cgacaggccg
     1201 acccttgcaa tcccgataac tcgagtccgg ttctcactag ggcctcgatt ggacccgggc
     1261 atcccgaaga attgttcctc gatcaggcgg acttcggcag agagaatcgg ggcgtagaac
     1321 tccaggaacg actcgccccc ggcgcccttg agggtcctgc tgacaggttg gcgggtggcc
     1381 aggatctgcg tgcggatctc attgctcaag gtgtcggcgg tcaaggtcgg gccgggttgg
     1441 gtcagggccc tgcctccacc gatcagaagc gttccagcat cgttgtaaat aaagacgtag
     1501 gcaacatctt cttcaccggt gatcccgcgt aatgaggcac tcagaaatcg ctcgtcctca
     1561 ctgaacacgc ccagttcgcc gctggcggcc agattactgg cgagagcctt tccatgtttc
     1621 acgaactctc gatgcagcaa tctattgtgc tgatagagcg acattccacc aaagaggacg
     1681 cagagcgcca ggatcatcgg aatgatcgac cacaagacct tcgttctgaa tttggtcatc
     1741 cgtatgagta ccatctgttc accctttatg ccaccccggg tctcccttga gctatcggat
     1801 caccacgact gcccggtcga ggagctcctt cgggatgtgt acgcccaact tgccggcggt
     1861 tttcagattc accgtcaacc ggacttttct cgctgtcgtg aacggaatct cttcgacgct
     1921 tctgccggaa aggatcccgt tcgccagctc agcggcctgg cttccgatat cccatccgct
     1981 ttcaaaggag agactgagta gggtgcccat ccgagcctga ttttctgaga gacccagaac
     2041 aggaatctta tgtctgaagg agaagagcag catatactgg accgattccg gcgccagaac
     2101 ggtcagatcc ggcaagatcc agagggcgtc gatctcatcc tgcatggcat cgagggtagc
     2161 gaacgagtcc ttgggtgagg tgatggcttt tgtgacgagc ctgagaccct gatctttggc
     2221 gattcgttca gcctgtctca ctaaaaagcc ggtctgagag ggatcataaa tgacgccgat
     2281 tcttcttacc tggggcgaga ctttttttag cagggcaagc tgttgctcat tgggaacatt
     2341 catactggcc ccggtgacat tgcggacttc ttgtctgatc actgcggtag ggttcaacac
     2401 catggcaaag acaactggga tatcttgtat ctcttcgagt acaacctgta aggcccagat
     2461 ccctaccgca aggatgagat ccggcttggg accggacttg agtcgtgcca ggaatttctt
     2521 acccttctgg aggtctccct ccatatcgta ctcaacgata gtcgactccc gcaaaacgct
     2581 cttgaacccc tccatcgctt catcatagac atcgacgtcg gcgctcttga gcgcaataat
     2641 ctttgctgcc tctgccggcg tcgaggagtg aataatcgct ccaacgatga cgcaagccgc
     2701 aaagataagg gaagcagtac gagttgtcag gagtgacatt caggagatcc tgggacctta
     2761 gaaaagatac tcgaccccta ttgtgggaat aaaatccgcc cggaggccgc tatttttgtc
     2821 cagtgggatc tggacgttcg cccgaaagat caaggaattg agaggattcc attttacgcc
     2881 gagggacagg tccacggtgt ggtccccggt tccatttcct tcaggcttct ggcgacctat
     2941 cacgtcggcg tcaaacgata gccctgatag gaggcgcgcg tcgaaaccga gggcatacct
     3001 gatggtgttc tgctcaagct ccttagtatt gatctcatag ccaacgttga cgtgaggcgt
     3061 gagccatcgc ccataggttt tagatgctat aaggaggcca gccaagttcg tcccgccggt
     3121 tcccaggagc cgtttcgcat ctcctgtcgg aagcttcact tcgaacagaa atgccaggtc
     3181 cggggtcaag ccgccggagt tgcgcaagaa gttgtatttc gttcgaagga tgatatcccc
     3241 gaggccggtc tcctcaccac ctccggaggc gtcgggtgga gtcgaattag gtccgaactg
     3301 gtggatcggg aggttgccaa tttgggtagt gcctggcttg gtgacaccga gagtgtcaac
     3361 atggaagata tcagcagaag ccctggcaaa gagccgaaca tggataaggg ggacgacgag
     3421 ccc
//
LOCUS       DATMXI010000178         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_309900, whole genome shotgun sequence.
ACCESSION   DATMXI010000178 DATMXI010000000
VERSION     DATMXI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309900"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VN444_03330"
     CDS             <1..700
                     /locus_tag="VN444_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWR20876.1"
                     /translation="SSVSRKLATLRSFFRYLCREGVLQANPAKLVPTPKLPKRLPAYL
                     SVDEVDRLLVSPSEEDPAGARDLAILELFYASGIRLSELTGLDVRDMDIRESLVRVKG
                     KGNKERIVPVGSKATAALRRYLGRRSDLIQGAKRETPEPLALFLNRQGGRLSQRGAAR
                     IVLKYLNRSGLGPKITPHGLRHSYATHLLQAGADLRAIQELLGHSRLSTTQRYTHLNL
                     DHLMEVYDKAHPRA"
     gene            720..1244
                     /gene="hslV"
                     /locus_tag="VN444_03335"
     CDS             720..1244
                     /gene="hslV"
                     /locus_tag="VN444_03335"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWR20877.1"
                     /translation="MRSTTILAVRHKGRVVVAGDGQVSFGGTVMKHTARKVRRMWNDR
                     VVTGFAGAAADAFALFTKFEAKLEEYGGNLPRAAVELAKDWRTDRALRRLEALLVVVD
                     QEHSLVISGTGDVIEPDDGVIGIGSGGPYAAAAARALVGFSELDARRIAEEAMKIAAS
                     ICVYTNDTITIEEL"
     gene            1402..2757
                     /gene="hslU"
                     /locus_tag="VN444_03340"
     CDS             1402..2757
                     /gene="hslU"
                     /locus_tag="VN444_03340"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWR20878.1"
                     /translation="MEQLTPRQIVAELDKYIIGQKDAKRAVAIALRNRWRRQRLPEDL
                     RDEVAPKNIIMIGPTGVGKTEIARRLAKLVDAPFIKVEASKYTEVGYVGRDVESMVRD
                     LTALAVDMVKEERTKAVQERARELAEERLLDLLLPVGRVATTPGVEQASDVSAITSAA
                     ETREKLRKRLHEGKLDDRTVELEVKDRGTPMVEIFSNSGIEEMDINVKEMLGNLMPQR
                     AKRRKVKVREAQRILAQEEGDKLIDIDEVRSEAVRRVEDSGIIFLDEIDKIAGRESSH
                     GPDVSRQGVQRDLLPIVEGCTVTTKYGLVRTDHVLFIAAGAFHVTKPSDLIPELQGRF
                     PLRVELASLTQGDFVRILTEPRNALIKQYVALLETEEVRLDFSEDAVQEIAAIASTVN
                     QATENIGARRLYTILERLLEEISFEAPAMQGARVDINAAYVRERLQEIVKNEDLSRYI
                     L"
BASE COUNT          590 a          742 c          946 g          529 t
ORIGIN      
        1 aagtagtgtc tcgcggaagc ttgcgaccct gcgatcgttc ttccgatatc tgtgccggga
       61 gggcgtactt caggcgaatc cggcgaagct tgtgccgacg ccaaaactgc caaagcggct
      121 tccggcgtat ctttccgtgg acgaggttga tcgcctcctg gtgtcgccca gcgaggagga
      181 tcccgcgggc gcccgcgatc tggcgatcct cgaactcttc tacgcctcgg gtatccgatt
      241 gagcgagctt acggggttgg atgtgcggga catggacatt cgggagagtc ttgttcgggt
      301 aaaaggcaag gggaataagg agcggatcgt cccggtggga tcgaaggcga ctgccgccct
      361 gaggcgctat ctcggtcggc ggagtgatct gatccagggg gcgaagcggg aaacccccga
      421 gccattggcg ttgttcctca accgccaggg cggccgtttg agccaacgtg gcgccgctcg
      481 aatcgtattg aagtacctca atcggagcgg cctgggtccg aagatcacac cgcacgggct
      541 gcgccatagc tatgccacgc atctgcttca agccggggcc gacctccgag cgattcaaga
      601 gttgctgggt cattcacgtc tctcaaccac ccagcggtat acccacctga atctggacca
      661 cttgatggag gtctatgata aagcgcatcc gagagcctga agagaaaggg taccctaaga
      721 tgcggagcac cacgatcctg gcggtgcggc acaaggggag ggtggtggtg gccggggacg
      781 gacaggtctc cttcggcgga acggtgatga agcacactgc gcggaaggtc cgtcggatgt
      841 ggaacgatcg ggtggttacc ggctttgccg gtgccgccgc cgatgcgttt gccctcttta
      901 ccaagttcga ggccaagctg gaggagtacg gcggcaacct gccgcgcgct gcggttgagt
      961 tggcaaagga ttggcgcacc gaccgggccc tccgccgatt agaggcgctt ttggtggtgg
     1021 tcgatcagga gcattcactg gtcatctctg ggactggtga tgtcatcgag cccgacgacg
     1081 gggtgatcgg tatcggctcc gggggcccat acgcagccgc cgcggctcga gcccttgtag
     1141 gcttctcaga gcttgacgcg agaaggatcg ctgaggaggc gatgaagatt gccgcctcaa
     1201 tctgcgtcta taccaacgac acgatcacga tagaagagct gtagataaac atcagcgttc
     1261 agcggttagc gatcagcggt cagcagagct tctgaagacg caaggttgta cccagaggtt
     1321 agtcacaagt tgtagccggg ttctggttgt tccttgattg tgctgaacgc tgatggctga
     1381 tagctgacct tggagcttct aatggaacag ttgacaccgc gacagatcgt ggccgaactt
     1441 gataaataca tcatcggtca aaaggacgca aagcgggccg tagccattgc gctgcgcaat
     1501 cggtggcggc ggcagaggct gccggaggac ctgcgtgatg aggttgcgcc gaagaacatc
     1561 attatgatcg gcccgaccgg cgtgggcaag acagagatcg cccgccgcct ggccaagctg
     1621 gtggatgccc cattcatcaa ggtggaggcc agtaagtaca cggaggtggg ctacgtagga
     1681 cgcgatgttg aatcgatggt gcgagacctg actgcactgg cggtggatat ggtgaaggag
     1741 gaaaggacca aagcagtaca ggagcgggcg cgggagttgg cagaggagcg gcttctggac
     1801 ctgctccttc ctgttggcag ggtggcgacg acgccgggcg tcgagcaagc ctctgatgtg
     1861 agcgcaatca cctctgctgc cgagactcgt gagaagctca ggaagcggct gcatgaggga
     1921 aagctggatg accgtaccgt agagctggag gtcaaggatc gaggcacacc gatggtggag
     1981 atcttcagca actccggcat cgaggagatg gatatcaacg tcaaggagat gctggggaac
     2041 ctcatgcccc agcgggccaa acgacgcaag gtcaaggtcc gggaagccca gcggatcctc
     2101 gcccaggaag agggcgataa gctcatcgac atcgacgagg tgagatcaga ggcggtccgt
     2161 agggtcgagg attccgggat catcttcctg gacgagatcg acaagatcgc cggccgcgag
     2221 tcgtcgcacg ggccggatgt ctcgcgccag ggggtgcaac gcgatctcct gccgattgtc
     2281 gaggggtgta cggttaccac caaatacggc ctggtgcgga ccgatcacgt cctgttcatt
     2341 gcggccgggg cgtttcatgt caccaaaccg tccgatctga tcccggagtt gcagggacgt
     2401 tttccgctgc gcgtggagct ggcgagcctt acgcagggcg acttcgtccg gatcctcacg
     2461 gagccgcgga atgccttgat caagcagtat gtcgcccttc tggagacaga ggaggtgaga
     2521 ctggactttt cggaggacgc tgtgcaggag atcgccgcca ttgccagtac cgtcaatcag
     2581 gcaaccgaga atatcggcgc ccgccggctc tacaccatcc tggagcggct gctggaggag
     2641 atttcgtttg aggcgcctgc aatgcagggg gcacgggtcg atatcaacgc agcctatgtc
     2701 cgggagcggc ttcaggagat cgtgaagaac gaggatctga gccgctatat cttgtaagac
     2761 agcgatcagc tatcaggtga aagcgttgca agcagcggag taaatag
//
LOCUS       DATMXI010000179         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_237253, whole genome shotgun sequence.
ACCESSION   DATMXI010000179 DATMXI010000000
VERSION     DATMXI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237253"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VN444_03345"
     CDS             <1..993
                     /locus_tag="VN444_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR20879.1"
                     /translation="WRKLDRLMEDEAGDHRDEIVNEKLEAVGRHLSLVFHRFLSGEVK
                     GYPKISLTINGHPIAAFDPFCRKNPSTQVLPEEIVRIGEAEVRLQPYVLPHHSRLSAS
                     EYDYYQDRSDFISNQGGYVYRNGRLMAWGDWFRLVPKGEATKLARVQIDFPNSLDEAW
                     TIDIKKSRARPPHAVRERLRQIIARITGRSVTVHRGRGQRLFQETQAPFWERYADHDG
                     IRFAINGQHPLIALLGTRLSPEDTDLLRVLLDSIAASLPVEMIYSDYSTHPREINQKA
                     VDESQTLERLKSLRQVLYGDGPGDPDAFLQIVRSTHLFDGQIELAEKFISETFS"
     gene            990..>3151
                     /locus_tag="VN444_03350"
     CDS             990..>3151
                     /locus_tag="VN444_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HWR20880.1"
                     /translation="MSITVITEERNIANALISGLANMPQTPTREQVEEKAQQIAAIFG
                     YTADLRNIVTEAMISVDTRMGAGISLVDVTAKHDDQWVHKREDINWTYAKSYEEFLRN
                     DGWPPQMVQSLSDVTTRILGHLQDPLSEGTTWNRRGLVIGHVQSGKTANYTGLIARAA
                     DAGYKFIIVIAGIHNNLRKQTQQRVDEAFIGRSSDPEDRHNIGVGLAPGYPHPATLTN
                     INEDFNKNTAAKSGWKINDFSKPIILVIKKNVTTLTALHKWLKELNAEGDGRISDVPM
                     LLIDDEADNASINTNKEDLDPTRTNAMIRRILGLFAKSCYVGYTATPFANIFIHPDAY
                     DDDVREELFPRDFIYCLDAPTTYFGAEKVFLNEETSQSIVRPVDDCEDLIPYAHKRDD
                     PVHELPPSLYRALDGFIVARTIRNLRGQSGKHCSMMVNVSRFVPVQKAVRDFLSLREK
                     KIREAVRANYAMPEDVSSRNTYMQGLKHAFDAEYADAGFSWAEVKAVLNGVFEHLHLY
                     VINSKSDEVLDYTRYEKEGVGLTAIAVGGLSLSRGLTIEGLTVSYMYRNTRMYDTLMQ
                     MGRWFGYRPGFEDLCRVHLSPDSINWYSHIADAAEELVQQVKRMRRDGLSPKDFGLYV
                     RSHPDSLLITASNKMRSGQEVMVEQNFSGRLRESYIVSTDPDVNARNFALIANHWRNG
                     FGGRQEEPTEKGVIFRDVPVEAIDEFLTRFECHSSFAG"
BASE COUNT          720 a          915 c          899 g          617 t
ORIGIN      
        1 tggaggaagc tcgaccgatt gatggaggat gaggccgggg atcatcggga cgagatcgtc
       61 aacgagaaac tggaagccgt gggaagacat ctgtcccttg tcttccatcg attcctctcc
      121 ggcgaggtca agggatatcc caagatttcg ctcaccatca acggtcaccc gatcgcggca
      181 ttcgacccct tctgccgaaa gaacccttcc actcaggtgc ttccggagga gatcgtccgt
      241 atcggcgagg ccgaggtgcg cctgcagcct tatgtattgc cacaccacag tcgcctgtcc
      301 gcatcagagt acgactacta tcaggatcgc agcgatttca tctccaatca ggggggctac
      361 gtttatcgaa acgggcgcct gatggcctgg ggcgactggt tcagactggt gccgaaaggt
      421 gaggccacca agctggctcg ggtgcaaatc gacttcccca acagtctgga cgaagcctgg
      481 accattgata tcaagaaatc gcgtgcccgc ccgccccatg cggtgcgaga gcgcctgcgt
      541 cagattattg ccaggatcac tgggcgaagc gtgacggtgc atcgtgggcg tgggcagaga
      601 ctgttccagg aaacccaggc ccctttctgg gagcgctatg ctgatcacga cggtatccgt
      661 ttcgcgatca acggacagca cccgctcatc gcattgctgg gcacaagact ctcgcccgag
      721 gatactgacc tgcttcgtgt cctgctcgat tcgatagctg cctccctgcc ggtggagatg
      781 atctattccg attactcaac ccatccacgc gagatcaacc agaaggcggt ggatgaaagc
      841 cagactctgg agcgcctgaa gagcctcagg caggtgctct atggggatgg gccgggcgat
      901 ccggatgcct ttctccagat cgtgcgctcg acgcatcttt tcgatggcca gatcgagttg
      961 gccgaaaaat tcatcagcga gaccttttca tgagcatcac agtcatcacg gaagaacgga
     1021 acatcgccaa tgcgctcatc tcgggtttgg ccaatatgcc ccaaacgcca acgcgcgagc
     1081 aggtggagga gaaggcacag cagatagccg caatctttgg ctatacggct gacctccgca
     1141 atatcgtcac cgaggcgatg atctcggtag atacgcgcat gggagccggc atttcgctgg
     1201 tggacgtcac tgcgaaacat gacgatcagt gggtccacaa gcgggaagac atcaattgga
     1261 cctacgcaaa atcctatgag gagtttcttc gcaatgatgg ctggcctccg cagatggtcc
     1321 agtcgctgag cgacgtgacg acacgcattc tcggccacct tcaggatcca ctgagcgaag
     1381 gcacaacctg gaaccgtcgg ggcctcgtca tcggccatgt gcagtcgggc aagacggcca
     1441 actacacggg cctgatagcg cgtgcggccg atgccggcta caaattcatc atcgtcatcg
     1501 ccggcattca caacaatctg cgcaagcaga cacagcaacg cgttgatgag gccttcatcg
     1561 gccgatcaag cgaccccgag gatcgtcaca acatcggcgt cggcctcgcg ccaggctatc
     1621 cgcatccggc aacgcttacc aacatcaatg aggatttcaa caagaacaca gcagccaaga
     1681 gcggctggaa gatcaacgac ttcagcaagc cgatcattct ggtcatcaag aagaacgtca
     1741 cgacgctcac ggcactgcac aagtggctga aggagctgaa tgccgagggc gatggccgaa
     1801 tttccgatgt gccgatgctg ctgatcgatg acgaggcgga caacgcatcg atcaacacga
     1861 acaaggaaga tctggatccg acccggacga atgcaatgat ccggcgcatc ttgggcctgt
     1921 tcgcgaagtc gtgctatgtc ggctacacgg caactccgtt cgccaacatc ttcatccacc
     1981 ccgatgccta tgacgatgat gtgagggagg aactgttccc tcgcgacttc atctattgcc
     2041 tggatgcgcc aacgacatat ttcggtgccg agaaggtgtt cctcaacgag gagacgagcc
     2101 aatccatcgt gaggccggtc gacgactgcg aggacctcat cccctacgcc cacaagcgcg
     2161 acgatcctgt ccacgagttg ccgcccagcc tctatcgtgc actcgacggg ttcatcgtcg
     2221 cccgtaccat ccgaaatctg cggggacaga gcggcaagca ctgctccatg atggtcaatg
     2281 tgtcgcgctt cgtgccagtg cagaaggctg tgcgtgactt cctgagtctc agggagaaga
     2341 agatcaggga agcagtccgc gccaactacg cgatgccgga agacgtttcc tcaagaaaca
     2401 cgtacatgca gggcttgaaa catgccttcg atgccgagta tgccgatgcc ggcttctctt
     2461 gggcagaagt gaaggcagtc ctcaatggag tcttcgagca ccttcacctc tatgtcatca
     2521 acagcaagag tgacgaggta cttgactaca cccggtacga gaaggaaggc gtcggattga
     2581 cagcgattgc agtcggcggt ctgagcctat cgcgcggtct gaccatcgaa gggctgactg
     2641 tcagctatat gtaccgcaac acgaggatgt acgacaccct catgcagatg ggtcgctggt
     2701 tcggctaccg accgggcttc gaggacctct gccgtgtcca tctctcgccc gactccatca
     2761 actggtattc ccatatcgct gacgccgccg aagagcttgt gcagcaggtc aagcggatgc
     2821 ggcgcgatgg cctcagcccg aaggatttcg gcctttacgt ccgctcgcat ccggacagtc
     2881 tgctgataac tgcttccaac aagatgcgat cgggtcagga ggtcatggtc gagcagaact
     2941 tcagtggacg actgcgtgag agctacattg tctccaccga ccccgacgtg aatgccagaa
     3001 acttcgcgct gatcgccaat cactggcgga atggttttgg tgggcgacag gaagagccta
     3061 cggaaaaagg cgtcatcttt agggacgtgc cagtcgaggc aatcgacgag tttctcaccc
     3121 ggttcgaatg ccattcgtcc tttgcgggca t
//
LOCUS       DATMXI010000180         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_108928, whole genome shotgun sequence.
ACCESSION   DATMXI010000180 DATMXI010000000
VERSION     DATMXI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108928"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1444)
                     /locus_tag="VN444_03355"
     CDS             complement(107..1444)
                     /locus_tag="VN444_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWR20881.1"
                     /translation="MRSGDLFRKGRKCLVVVLTLVLLIIGGGNHEVTAVEDNYERLKV
                     FTEVLSLIQANYVEETKPGDLIYSGIKGMLEVLDPHSAFMPPDTFKEMQVETQGSFGG
                     LGIEIAVKDRMLTVVAPIEGTPADRAGIHPGDRIVKIDGNPTKDMTLMEAVKKLRGPK
                     GTSVTLAILREESPGPFELTLVREVIEVKSVKAKDLGDGIAYVRISAFQERTGKDLLK
                     AIEQLGHGVMSAMVLDLRNNPGGLLNQAVQVSDLFLDQGQLIVYTEGRLKNQNLRFSA
                     EHGAQFPRIPIVVLVNGGSASASEIVAGALQDWKRAVILGTKTFGKGSVQTVVPLSDG
                     SGLRLTTAKYFTPQGRSIHGTGLVPDIIVETPRPTVAKAQGGPGEKESEPGKERPEKR
                     QEKKISEEEGDASLQISRREGPDPNSDIQLKRAMEILKASQILEKGFVKGSAG"
     gene            complement(1514..2761)
                     /locus_tag="VN444_03360"
     CDS             complement(1514..2761)
                     /locus_tag="VN444_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWR20882.1"
                     /translation="MKTMARRSPLTLGVVVALMASLLLCPPSGVAGETRTGSSSRLRE
                     KREELKQVKKELDRERKQAQQVARKERSLSEELARIDRELQQKGRELKELQARLKIST
                     ERLQTTQRDIRLTRTHLDQIQGLFRMRVRAIYKQGRYGYVQALLSSENLSGAGRRVRY
                     LAAIANQDQRMVAAYTGAIETLGAQQAELERSKAELTMSQKAVTAKREEILEEQQARR
                     LLLANVQEQKKNHLSAVRELELAARELHGLIGRLQQQKRPQVSRAPHPESLRVPGLSE
                     GPVSAFALLKGRLPWPTTGDVVSTFGRQEHARYRTITFNRGVEIRAPEGQKIASVFEG
                     VVLYADWFKGYGRLIVLDHGEGYYTVYAHAAEILVKVGNRVAKGQAIGLVGNTGSVSG
                     SQLYFEVRHRGGPQDPVAWLAPN"
     gene            complement(2777..3673)
                     /gene="ftsX"
                     /locus_tag="VN444_03365"
     CDS             complement(2777..3673)
                     /gene="ftsX"
                     /locus_tag="VN444_03365"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWR20883.1"
                     /translation="MIEKLKHLLAHALINLARAGWGGVASIASITVSFVIIGIFLLLI
                     QHFNALVTEWKEQFQVSVFLDNQITPLQLEMLRRRIENEAAVKAFTFTTKEEALANFR
                     RELKGQESLLEGLGDNPLPASFQLKIREGYQSPEALKGLSAFLSRLEGVEDIQYWQEL
                     VERISHAARLIRLLGIIIGSVLTLGSAMIVSNIIRLAVYARAQEIEIMRLVGATKAYI
                     RAPFLVEGMLQGGLGAVLALGVLFAAYRSVAPTFNLASQLFPAAAGQGFLESSMMAGL
                     VGGGAVIGALGSSIAVSRFLKM"
     gene            complement(3740..4444)
                     /locus_tag="VN444_03370"
     CDS             complement(3740..4444)
                     /locus_tag="VN444_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR20884.1"
                     /translation="MIQMFHVYKTFGKDLEALVDVDLHIHKGEMVFLAGPNGAGKSTL
                     LRLIFCDEFPTSGQILVNGRTTVHMKPRAIPYLRRTMGIVFQDFKLLRDRTIEENLAL
                     VALVAGGTAIQARQKVAQLLKLVGLSHKAQMVPCKLSSSEQQRVAMARALMNDPLILL
                     ADEPTGNLDTEQAADAIRLLFEINAQGTTVLVATHNLKLAEEAGCRTVFLKQGRIVED
                     RQRVNLPTGPAQQKRV"
     gene            complement(4626..5525)
                     /locus_tag="VN444_03375"
     CDS             complement(4626..5525)
                     /locus_tag="VN444_03375"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWR20885.1"
                     /translation="MTQKPQQFAGADLLLGAHMSIAGGVDLAPLRGQQVGCRTIQLFT
                     KSSNQWRARSLPSEEIDRFRANLQAAAIAPAVAHGAYLINLASTDPALHQRSMAACLE
                     ELERAEALGIPYLVIHPGAHMGAGEEAGIRQVANSLRELLKQTKGCRVQVVLETTAGQ
                     GTALGHRFEHLALLLDQIDLPERTGVCLDTCHLFAAGYDIRTFDDYYGVLRLFDQVVG
                     IPSLKVIHVNDCKKELGCRVDRHEHIGKGTIGLEAFRCLVTDPRLRGIPMIIETPKDD
                     DFVTADRRNLETLRGLAEEQRAE"
     gene            complement(5557..5796)
                     /locus_tag="VN444_03380"
     CDS             complement(5557..5796)
                     /locus_tag="VN444_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20886.1"
                     /translation="MPTSEELKEEERKLRQLRVVVSLTMSTISQTTLSLEEASKMVTA
                     TRALALRLFPGKELAFELIYQPRFRRLLAERFRLH"
     gene            6103..6375
                     /locus_tag="VN444_03385"
     CDS             6103..6375
                     /locus_tag="VN444_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWR20887.1"
                     /translation="MKIRVERNPSPEQLATLKIEDWPIWSKEVSIFPWSYDSTETCYI
                     LEGEVIVTPEGGEPVQIGKGDLVTFPVGMACTWDIRRPVKKYYQFD"
     gene            6405..>6635
                     /locus_tag="VN444_03390"
     CDS             6405..>6635
                     /locus_tag="VN444_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWR20888.1"
                     /translation="MARILAIGTATLDLIFTLTHYPAEDVELRAIELRTSPGGNAANT
                     LVVLSQLGHACAFGGVLADGSEAAPILECMARH"
BASE COUNT         1312 a         2058 c         1858 g         1407 t
ORIGIN      
        1 gtttcaatcc ctaaggttag gtgtgccatt gtgaagagca gctatcagct tttagcagtc
       61 agcttgaagc tgatagctga tcgctgatag ctgatcgctg catttatcac cctgcactcc
      121 ctttcacgaa tcccttctcg aggatctggc tcgccttgag aatctccatc gcccgcttta
      181 actggatgtc gctgttggga tcgggtccct cgcgcctgct aatctggaga ctcgcgtctc
      241 cctcctcttc ggaaatcttc ttctcctgcc gtttctcagg ccgctccttt ccaggctcac
      301 tctccttttc accggggcca ccctgcgcct tggccacggt gggccgtggc gtctctacga
      361 tgatatcagg cacaagcccg gtgccgtgga tcgaccgtcc ctgcggcgtg aagtacttcg
      421 ccgtggtcaa gcgcaggccg gaaccatcac tcagcggaac aaccgtctgt accgacccct
      481 tcccgaaggt ctttgtcccg aggatcaccg ctctcttcca gtcctgaagg gcgcccgcga
      541 cgatctcgga ggcgctggcg gagcccccat tcaccaggac cactataggg atcctgggaa
      601 actgagcccc gtgttcggcc gagaagcgga gattctggtt cttgagccga ccctcggtgt
      661 agacgatgag ctgcccttgg tccaggaaca gatcggacac ctggacggcc tgattcagga
      721 ggccgccagg gttgttgcga aggtccagca ccatggccga catcacgcca tgtcccaact
      781 gctcgatcgc cttcagaagg tctttcccgg tccgctcctg gaacgcgctg atccggacat
      841 aggcgatccc atcgcccaga tccttggcct tgacgctctt cacctcgata acttcccgaa
      901 ccaacgtcag ctcgaacggg ccgggggact cctcacggag gatagccagg gtgacgctcg
      961 tgcccttggg gccacggagc tttttcaccg cctccatgag ggtcatgtct ttagtcgggt
     1021 tcccgtcgat cttcacaatc cggtcgcccg gatgaatgcc ggctcgatcg gctggggtcc
     1081 cttcgattgg cgccaccacc gtgagcatcc ggtccttgac cgcgatctcg atccccaggc
     1141 cgccgaacga accctgggtt tcgacctgca tctctttgaa ggtgtcgggc ggcatgaatg
     1201 cgctgtgcgg atcgagtacc tccagcatcc ctttaatgcc gctgtagata aggtcaccgg
     1261 gcttcgtctc ctccacgtaa ttcgcctgga tcagcgacag aacctcggtg aagaccttca
     1321 gccgctcata gttgtcctcg acagccgtga cctcatggtt gccgccgccg atgatcaaca
     1381 gcaccagggt caatacaacg accagacact tccgcccctt ccgaaaaaga tctccagatc
     1441 tcatcgattc ctcccagtta accagctatc agcattcagc tatgagctgg cggctgatag
     1501 ctgaatgctg catttagttg ggggcaagcc aagccacggg atcctggggc ccgccacgat
     1561 gccgcacttc gaaatagagc tgcgatccgg acactgaccc cgtgttaccc accaacccga
     1621 tagcctgtcc tttcgccact cgattgccca ccttgacgag gatctcggcg gcatgcgcat
     1681 agacggtgta gtagccttcc ccgtggtcaa ggacgatcaa gcggccatac cccttgaacc
     1741 aatcggcgta cagcacaaca ccttcaaaga ccgaagcgat cttttgcccc tcgggagcac
     1801 ggatttcgac gccgcggttg aaggttatcg tcctgtagcg cgcatgctcc tgccggccga
     1861 aagtagagac cacgtcgccg gttgtcggcc acggcaagcg gcccttgagc aatgcaaacg
     1921 cgctaactgg tccttcagag aggccaggta cccgcaacga ctccgggtga ggcgctctgc
     1981 tgacctgagg ccgcttctgt tgttgcaggc gaccgatgag accatggagt tcccgggccg
     2041 cgagctccag ctctcggacg gcggacagat gatttttttt ctgctcctga acattggcta
     2101 gcaacagacg tctggcctgc tgctcctcta agatctcttc gcgctttgct gtgacggcct
     2161 tctggctcat agtcagctca gccttcgatc gttccagctc ggcctgttgg gcgcccaggg
     2221 tctcgatcgc acccgtgtag gcagcaacca tccgctggtc ctgattcgca atcgcagcaa
     2281 ggtatcgtac gcggcgccct gcgcccgata gattctccga ggacaggagg gcctggacgt
     2341 agccataccg cccctgcttg tagatcgcgc ggacacgcat ccggaacagg ccctgtatct
     2401 ggtccaggtg agtccgggtc agcctgatat cgcgctgggt ggtctgaagc cgttccgtac
     2461 ttatcttcag cctggcctgc agctccttta gctcccgtcc cttctgctga agctcccgat
     2521 caatccgagc caactcctcg gaaagtgatc gctccttccg cgccacctgc tgcgcctgct
     2581 tccgctcccg atccagttcc ttttttacct gcttgagctc ttcgcgcttc tcgcggaggc
     2641 gagaagatga tcccgtcctt gtttcgcctg ccacgccaga tggcgggcag aggagcaggg
     2701 acgccatgag agccaccact accccaaggg taagaggcga acgacgagcc attgtcttca
     2761 ttcgagcgat tgctggctac atcttcagga agcggctgac agcgatcgag ctacccagtg
     2821 cgccgatcac tgcgcccccg cccaccaaac ctgccatcat cgacgattcc aggaatcctt
     2881 gacccgccgc tgccgggaag agctgggagg ctaaattgaa ggtcggtgcg acggatcggt
     2941 aggccgcgaa cagtacaccc aaggcgagca cggcgccaag gccgccctga agcatcccct
     3001 ccaccaggaa gggggcacgg atataggcct tggtggcacc tacgaggcgc atgatctcaa
     3061 tctcctgtgc acgggcatag actgcgagtc gaatgatatt cgagacaatc atggctgatc
     3121 ctagcgtcag caccgaaccg ataatgatgc cgagcagcct gatgagccgt gcggcatggg
     3181 aaatacgctc gaccaattcc tgccagtact ggatatcttc caccccctca aggcggctca
     3241 ggaacgcgct gagccctttg agggcctccg gactttgata cccctcgcgg atcttgagct
     3301 ggaatgaagc gggcagcgga ttatcgccta ggccctcgag gagactttcc tgccccttga
     3361 gttcccggcg gaagtttgcg agcgcctctt ccttcgttgt gaaagtgaag gccttgacgg
     3421 ccgcctcgtt ttcgattcgt cgcctgagca tctctagctg caacggggtg atctgattat
     3481 ccagaaagac agacacctga aactgttcct tccactcagt aacaagcgcg ttgaaatgct
     3541 ggatcaaaag gagaaagatg ccgatgatga caaacgacac tgtaatgctg gcgatcgacg
     3601 ccaccccacc ccacccagcg cgagccaggt tgatcagagc atgagccaat agatgtttca
     3661 gcttctcaat catcagccaa atggaagatc tacagaacga gatgagagac gagccggttt
     3721 gaaccctcta ccatacgccc tacactcttt tctgttgagc cggacctgtc ggcaggttca
     3781 cccgctgccg gtcctcgacg atccttcctt gtttgagaaa tactgtgcgg cagcccgcct
     3841 cctctgccag tttgagattg tgagtggcca ccaggacggt ggtcccctgc gcgttaatct
     3901 caaaaagaag tcgtatggca tctgcggcct gttcagtatc cagattccct gttggctcgt
     3961 cggccaggag gatgagagga tcattcatta gcgccctcgc catcgccacc cgctgctgct
     4021 cgctgcttga aagcttgcac ggaaccatct gtgccttgtg agaaagcccc accagcttca
     4081 acagttgtgc gaccttttgc cgtgcctgta tcgccgtccc gccggccact agggccacca
     4141 gggcaagatt ttcctcgata gtccgatcgc gaaggagctt gaaatcctga aagacgatcc
     4201 ccatagtccg cctcagatac gggattgccc tcggtttcat gtggacggtg gtgcggccgt
     4261 tgaccaggat ctgaccggag gtaggaaatt cgtcacaaaa aatcaagcgg agtagagtgc
     4321 tttttccggc accgttggga ccagccagga agaccatctc ccctttgtga atatgaagat
     4381 cgacatcgac caaggcctcc agatcttttc caaacgtctt gtacacatgg aacatctgga
     4441 tcatagcgaa acagccctgg ttgacctcct tgcatcttct cttccggtag cccgcggcag
     4501 ggcccaggcg cgaacctatt tccacctctc cttgcggccc tcacgttagc cccttttcag
     4561 aatcaatgca actaaaaatc ttcccggcga tggcggagcg atcaatgaag tcacacggca
     4621 ggctatcact cagcccgctg ctcctccgcg agacctcgga gggtctccag attccggcga
     4681 tcggctgtta cgaagtcgtc gtccttcgga gtttcgatga tcatcgggat cccgcgtagg
     4741 cggggatcag tgaccaggca gcggaacgcc tcgagaccga tcgttccctt gccgatgtgc
     4801 tcgtgacggt ccacgcgaca tcccagctcc ttcttgcagt cgttgacgtg aatgaccttg
     4861 agggagggta tgcccacgac ctgatcgaac agacggagga caccgtagta atcgtcgaag
     4921 gttcggatgt cgtagccggc ggcaaacaag tggcaggtat ccaggcagac gccggttcgc
     4981 tccggaagat cgatctgatc caggagcagc gcgagatgct cgaaacggtg ccccagggcg
     5041 gttccctggc cggcggtcgt ctcgagaact acctggaccc gacagccctt cgtctgcttc
     5101 aagagctccc ggagactgtt cgccacctgt cgtattcccg cctcctcgcc ggcccccatg
     5161 tgggcccctg gatggatcac cagatatggg atgcccagtg cctcggctcg ctcgagctcc
     5221 tccaggcagg ctgccatcga ccgctgatgc aacgcggggt cggtggaggc tagattgatc
     5281 aggtaggcgc cgtgggccac ggcaggagcg attgcagcag cctgaagatt tgcgcggaat
     5341 cggtcgatct cttcggaggg caaagacctg gcccgccact ggttgctgct tttggtgaaa
     5401 agctggatgg tccgacagcc gacctgctgg cccctcagcg gcgccagatc gacccctccc
     5461 gcgatcgaca tatgggcgcc aaggagcaga tcggccccag cgaactgttg cggtttctgt
     5521 gtcatcacta gtgaggatgc cgaggggatc ccaacctcaa tggaggcgga accgttcggc
     5581 caggagcctc cggaagcggg gctgatagat cagctcgaag gccagctcct tcccgggaaa
     5641 gaggcgcagc gccaacgccc tcgtggcggt gaccatcttg gaggcctcct ccagcgaaag
     5701 tgtcgtctga ctgatcgtgc tcatcgtgag gctgaccacg acccgaagtt ggcgtaactt
     5761 tctttcctcc tccttgagtt cctcagatgt tggcatggcc tatcctcctc tgccgttcac
     5821 tgccatgata acaaagagat ccctcgtttc caagggtcgt ccgagaaggc gagaatagat
     5881 tatagcgcaa ctctttcatt ggtccttcat tgcgagtgac cgaagggagc gcggcgatct
     5941 cactgtactt tcactgagcg attgcttcgt cgcatccgct cctcgcaatg acgcttcgat
     6001 gaatgggttt ggaatagttt gggcttgacc gcttcgccag gggcatctta ccatgcggcc
     6061 actggctgag gggacatgct gaaacgaaag gaggacggat gtatgaagat ccgagtggag
     6121 cgcaaccctt ccccggagca actcgcaacg ctcaagatcg aggactggcc aatctggagt
     6181 aaagaggtct ccatctttcc ctggagctac gacagcacgg agacttgtta tatcctggag
     6241 ggggaggtga tcgtgacgcc agagggcggg gagccggtgc agatcggcaa gggggatctc
     6301 gtcacctttc ccgtcgggat ggcctgcacc tgggacatcc gccgccccgt caaaaagtat
     6361 taccagtttg actgatctgg ctgccaccac aagggggaac cggcatggcg cgtatcctgg
     6421 ctatcgggac ggcgacgctt gacctcatct tcaccctcac gcactaccct gctgaggatg
     6481 tcgaactccg cgccatcgag ttgcgcacga gtcctggtgg taatgccgcc aacaccctgg
     6541 tggtcctcag tcaactaggt catgcgtgcg cctttggtgg ggtactggcg gatggttcgg
     6601 aggccgcccc gatcctcgag tgcatggcca ggcac
//
LOCUS       DATMXI010000181         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_157975, whole genome shotgun sequence.
ACCESSION   DATMXI010000181 DATMXI010000000
VERSION     DATMXI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157975"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VN444_03395"
     CDS             complement(<1..365)
                     /locus_tag="VN444_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWR20889.1"
                     /translation="MPQVLIQRLVQPPIGEQRIEIVERKGLGHPDSICDAVMEEVARA
                     INADYQQRFGRVLHNNIDKGLLVAGRVRRRFGGGRVLRPMELVIGDRATFRVGKVRVP
                     IKEIAVEAAKEWFRKSLRHV"
     gene            complement(377..892)
                     /locus_tag="VN444_03400"
     CDS             complement(377..892)
                     /locus_tag="VN444_03400"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HWR20890.1"
                     /translation="MKTPVIARKRTASFRKKVDTETDSYLAKGAPGKMVACHECHALS
                     TGKRWYQDEAVYTKLLKAGTVTEVFCPACEKIRDSYPNGQVTLKGPFLAEHHDEILHI
                     ITNEEKRARKLNPLHRIMSLREENGQLEVTTTDEKLAQRIGRELHKACGGTVAYGWSH
                     NDKFLRVQWER"
     gene            complement(979..1425)
                     /locus_tag="VN444_03405"
     CDS             complement(979..1425)
                     /locus_tag="VN444_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWR20891.1"
                     /translation="MMALVKWTPFGDLPVFRREVERLFDRFLGEMPRLDLSGMGWTPH
                     LDVTETKESLVIKAELPGLDVKDLDITISGNTLTLKGEKRQAKEEKDEHHHLIERSYG
                     AFTRTVELPAPVAADKVKAAFKNGVLTVTLPKTEEAKRKPISIEVD"
     gene            complement(1531..2397)
                     /locus_tag="VN444_03410"
     CDS             complement(1531..2397)
                     /locus_tag="VN444_03410"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWR20892.1"
                     /translation="MAWVAWLTGLPGSGKSSVGREVARRLEERGILVRVLELDEIRRV
                     VTPSPSYTAEEREVLYRALAYMAWLLYNEGVSVIIDATAHRRRFRDVARALIPAFAEI
                     FIRASLPACLARAGYRHGGHAPFDVYGQAGRKGSTVPGVDEMYEPPLHPELVLDTEEL
                     QVTQAADRVVRFLETFQVGQATVSLNADYGQADRGGGMMNVLSDLQAPDSAGEQGERS
                     EACPYLRRTTPNSCYCIEGYCLAHPYGGLQVVTVAEFREMCTQAEYVRCQLYRRRHAQ
                     TVVDEDTERAAA"
     gene            complement(2408..>3236)
                     /locus_tag="VN444_03415"
     CDS             complement(2408..>3236)
                     /locus_tag="VN444_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWR20893.1"
                     /translation="ATYGKLPRHVKSLDVGFFTGEGQLQSAGRAEEYFLLVEKVEGSV
                     YRVDLERIRTEGKATASDFDRARALSGYLAGIHAEKGQDVQLYFRRIRDLIGHGEGIM
                     GILDGYPADYLLLSSKQQYLIESGCMAWRHYLKEKAERLSIVHGDFHPWNILFREGTD
                     FTLLDRSRGEWGEPADDIAALSINYLLFSLLRSGQMEGPFRELFDLFYGEYLERTHDL
                     DLNSVIPPFYVFRALVIASPRWYPDLPEPVRVKLFRFAQAMLRVEQFDHKDLNRYLT"
BASE COUNT          657 a         1058 c          836 g          685 t
ORIGIN      
        1 acgtgacgca acgacttgcg gaaccactcc ttggccgcct ccaccgcgat ctccttgatc
       61 ggaaccctca ccttaccgac gcggaaggtg gcgcgatcgc cgatcaccaa ctccatcggt
      121 cgaagcacgc gccctccgcc gaacctccgg cgcacccgcc ctgctaccag gagccccttg
      181 tcgatattat tgtggaggac tcggccaaag cgttgttgat aatcggcatt gatggcccgt
      241 gccacctcct ccatcaccgc atcgcagatc gagtccggat gacccaaccc tttccgctcg
      301 acaatctcta tccgctgctc gccaatgggc ggctgcacta atcgctgaat aagaacctgc
      361 ggcatcggtc ttcccttcag cgctcccact gcactcgtag aaacttatcg ttgtgcgacc
      421 agccgtatgc gacggtcccc ccgcaggcct tgtgaagctc ccgtccgatc cgctgggcca
      481 gtttctcgtc ggttgtcgtg acctcaagct gcccgttctc ctcacggagg ctcatgatcc
      541 gatgaagcgg gttgagcttc ctggcccgtt tctcctcgtt ggtaatgatg tgcaggatct
      601 catcatgatg ctcagccagg aatggtccct tgagcgtcac ctgcccgttc gggtagctgt
      661 cgcgaatctt ttcacacgcc ggacagaaca cctctgtgac cgttcctgct ttgaggagct
      721 tggtatagac cgcctcatcc tgataccatc gcttccccgt cgagagggca tggcactcgt
      781 gacacgccac catcttgccg ggggctccct tggccaggta ggaatcggtc tcggtatcca
      841 ccttcttcct gaacgatgcg gtccgcttgc gcgctattac tggtgttttc atcgatctct
      901 ccctgtaact gggttccacg tttcaggttc caggaacctg aaacgtggaa cctcaaacat
      961 ggaacgtgaa accggttctt agtcaacctc aatcgagatc ggcttccgct tggcctcctc
     1021 cgtcttcgga agggtaacgg tcaacacccc gtttttaaag gcggccttaa ctttatcagc
     1081 ggcaaccggc gccggcagct cgaccgtcct ggtaaaggcg ccataggatc gctcaatcag
     1141 gtggtggtgt tcgtccttct cctctttcgc ctgtcgcttc tcccccttca gggtcagcgt
     1201 attcccggag atggtaatat ccaggtcttt gacatcgaga ccgggaagct cagccttgat
     1261 aaccagagat tctttagttt cagtcacatc cagatgcgga gtccacccca tcccggacag
     1321 gtccagccga ggcatctcac ccaagaaccg atcaaataac cgctctacct ctcgcctaaa
     1381 aacaggcagg tctccgaatg gtgtccactt tacgagtgcc atcatccttc ctccttcctt
     1441 cgaaaatcac ctattccgtc tatctggtct gtctcgtctg ttcggtcatt tggtctagac
     1501 caaatggacc agatagagcc agaattacat tcaggctgct gcgcgttccg tatcctcatc
     1561 gactactgtc tgagcgtgcc gcctgcggta cagttggcac cgaacgtact cggcctgtgt
     1621 gcacatctcg cgaaactcgg caacagtcac aacttgaagc cccccataag ggtgcgccaa
     1681 gcagtatcct tcgatgcaat agcaggagtt gggggtggtt cgtcgcagat aggggcacgc
     1741 ctcacttcgc tcaccctgct cacctgcgct atccggcgcc tgtaggtccg acagcacatt
     1801 catcatgcct cctccgcggt cagcctgtcc ataatccgcg ttcaacgaaa ctgtcgcctg
     1861 gccgacctga aaggtttcaa gaaacctcac cacccgatcc gcagcttgcg tcacttgaag
     1921 ctcctcggta tcaagtacga gctcagggtg aagcggcggc tcgtacatct catcgacgcc
     1981 cggaacggtc gatcccttgc ggcccgcttg cccatagaca tcgaaagggg cgtggccgcc
     2041 atgccgatac cccgcccgcg ccagacaagc cgggagcgac gcgcggataa agatctcagc
     2101 aaaggccggg atcagcgccc tggcgacatc gcggaaccgt cgtctgtggg cggtcgcatc
     2161 gatgatcacg ctcaccccct cgttgtagag aagccacgcc atataggcga gggcgcgata
     2221 gagaacctcg cgttcctcgg ccgtatagct cggtgagggg gttaccaccc ttcggatctc
     2281 atcgagctcc aatacccgca ccagaattcc gcgctcctcc aggcgccgag cgacctcacg
     2341 ccccacgcta ctcttgccgc ttccgggaag tcctgtgagc cacgccaccc acgccaccgc
     2401 aatctcctca cgtcaagtat cgattgagat ccttgtgatc gaactgctca actcggagca
     2461 tcgcctgggc gaaccggaat agcttcacgc gcaccggctc cggaaggtca ggataccagc
     2521 gcgggctcgc gatcactaaa gcccgaaaga cgtagaaggg cgggatgacg gagttgagat
     2581 ccagatcgtg tgtccgctca aggtactcgc cgtagaagag atcgaacagc tcccggaatg
     2641 gcccctccat ttgccccgac cgcaagagcg agaagagaag ataattgatg ctcagggccg
     2701 cgatgtcatc ggccggctcc ccccactccc cacgactccg gtcgagcagc gtgaaatcgg
     2761 tcccttctcg aaagaggatg ttccaggggt gaaaatcgcc gtgaacgata ctcaggcgct
     2821 cggccttttc tttcagatag tgtcgccagg ccatgcatcc gctctcgatg aggtactgct
     2881 gtttggaaga gaggaggaga taatcggccg ggtacccatc caggatcccc atgatcccct
     2941 caccgtggcc gatcagatcc cggatccgac ggaagtacaa ctggacatcc tgacctttct
     3001 ctgcgtgaat ccccgccaga taccccgaga gcgccctggc gcggtcaaag tcggaagcgg
     3061 tcgccttccc ctcggttcga atccgttcaa gatcgacccg atacacagac ccctcaacct
     3121 tctctacaag gaggaagtac tcctctgcgc gcccggccga ttgcaactgt ccttcaccgg
     3181 taaagaagcc gacgtcaagg ctcttcacgt ggcgcggaag cttaccgtag gtggca
//
LOCUS       DATMXI010000182         2707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_45255, whole genome shotgun sequence.
ACCESSION   DATMXI010000182 DATMXI010000000
VERSION     DATMXI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45255"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN444_03420"
     CDS             <1..412
                     /locus_tag="VN444_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWR20894.1"
                     /translation="ITGLASHRRAAEELLSKLPRERILNLTGATSLRQLLEILAGARF
                     LLANDSGNLHMARLVGTLVVGVFGPTVPEQRFYDLSGGFMTIRHPLPCSPCAHTARYL
                     RCPGPYRQCLAELKGSAAEEALLSACRMVADRVA"
     gene            409..1515
                     /locus_tag="VN444_03425"
     CDS             409..1515
                     /locus_tag="VN444_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR20895.1"
                     /translation="MIRVAIVGCGRMGENHCRIVKELQEVSIVGAVDPNLDRAKALAA
                     GYAVEQAAAHLDELLARTHIDAVHILTPPPTHAPLACTALQAGCHVLVEKPMALTVVE
                     ATRMLDAATQHHRILTVGHNHLFESAVREAHARVAQGRLGRLVGLDVFHGCLPGSPPW
                     VADLPSGPWINDMSHPLYLSELFLGEPLTIRAVGHPPAGRQKVREVRAVAQHAEGVST
                     LTLSTDAAPFRHKITLFGTHRTLEVDLIHEILVETRPFGGHRWMRKGRAVLDVSTQLL
                     VGAGRNAYQVLAGQGRGWQGLRALIQAFYTAIQTGGPSPVPATQGLRVVTYLEEISRQ
                     LMAMPDPVQDAVGKTRDNALSACRTETDKWIGQL"
     gene            complement(1833..2273)
                     /locus_tag="VN444_03430"
     CDS             complement(1833..2273)
                     /locus_tag="VN444_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20896.1"
                     /translation="MEVVVKNPQFCQLQQGRQQRGSRSDNGCRVKIDQYQGRQVGPLF
                     PQEYIVRFQIQVKDLIPVQQTNHLGKTVDDLLPFGGFGLRQISNCGGCAVLLERHGIS
                     TPLCEDEAAPYSEETFFFDDGNDGRRVNTYALESLRDSKIAEGQ"
BASE COUNT          537 a          798 c          755 g          617 t
ORIGIN      
        1 tatcaccggt ctcgcgtcac accgcagggc ggcggaagaa ctgctgagta agcttcctcg
       61 tgagcggatc ctgaacctta caggcgccac ctcccttcgt cagttgttgg agatcctcgc
      121 aggagcgcgc ttcctgctgg ccaatgatag cgggaacctt cacatggccc gtctcgtcgg
      181 aacgctggtt gtgggcgtgt ttggtccgac ggttcctgaa caacggtttt acgatctatc
      241 aggaggattt atgacgattc gacacccgct gccctgttcg ccctgcgccc acaccgcccg
      301 gtacctcagg tgccctggac catatcgcca atgcctcgcc gagctgaagg ggtctgccgc
      361 tgaagaggcg ctgctgagcg cctgccgaat ggtggccgac cgagtcgcat gatccgggtg
      421 gcgatcgtag gctgcgggcg gatgggggag aaccactgcc gcattgtcaa agagcttcag
      481 gaggtctcta ttgtgggtgc ggttgatccc aatctcgaca gggcgaaagc gctcgcggca
      541 ggttatgccg ttgagcaggc ggcagcacac ctggacgaat tgctggcgcg tactcacatc
      601 gatgcggtcc atatcctcac cccgccgccg actcacgctc ccttagcctg tacagctctg
      661 caagcgggat gtcacgtgct tgtcgaaaaa ccgatggccc tcacagttgt agaggccacg
      721 cggatgctgg acgcggcgac acagcatcac cgtattctga ccgtcggtca caatcacctt
      781 tttgagtctg ccgtccgcga ggcccacgcc cgggttgctc agggtcgtct gggccgactc
      841 gttggcctcg atgtctttca tggatgtctt ccaggctccc ctccctgggt tgccgacctg
      901 ccgtccggac cctggatcaa tgatatgtcc catcccttgt atctgtcgga gctttttctg
      961 ggcgagccgt tgaccatccg ggcagtgggc catccccccg ccggccgtca gaaggtgagg
     1021 gaggtccgtg ccgtagcgca acacgctgag ggggtgagta cccttaccct ttccacggac
     1081 gccgcccctt tccgtcacaa gatcaccctc tttggaacgc accgcacgct cgaggtcgat
     1141 ctgattcacg aaatccttgt ggaaacccgt ccgtttggcg ggcatcgctg gatgaggaag
     1201 gggcgcgccg tcctggacgt cagtacccag cttctggtcg gtgccgggcg gaacgcgtat
     1261 caggtcttgg cgggacaagg acgaggatgg caggggcttc gcgcgctgat tcaggccttc
     1321 tacactgcga ttcagacagg gggtccttct cccgttcccg cgacacaggg gctccgcgtt
     1381 gttacctatt tagaggagat cagccggcag cttatggcaa tgccggatcc cgtccaagac
     1441 gcagtcggca agactcgtga caacgctctt tccgcctgcc gtacagagac cgacaaatgg
     1501 atcggacaac tatagacagc cctttaactg aagccgggtt gtgcgggtcg actgcgctcc
     1561 ggagaacggt gcaccaagat tacagggtct tccgaaccgg acggctgttt ggttacgttc
     1621 atgaagcgtt cgccggcaac ggcctgtgcg atctacttcc tggtctgctt gcatcgcctc
     1681 cgtctttaga aaaagttctc taccggagtt actcgaatag agatgtgttt atgattaatg
     1741 tcccgattcg cggcgaccta caggtcatgt gcctcaaaag tgttcgatgt aagagctggt
     1801 ggcgcctgat ctttgtagat cctattatgc cctcactggc cttccgctat cttgctgtcg
     1861 cggagactct caagggcata ggtattgaca cgccgaccgt cattgccgtc gtcgaagaaa
     1921 aatgtttctt cggagtaagg cgcagcttcg tcctcacaga gtggtgtcga aataccgtgg
     1981 cgctccaaaa gtacggcgca gccaccacaa ttcgagatct gtcgcaaacc gaatccgccg
     2041 aacggcaaga gatcgtcgac cgttttgcca agatggttcg tctgctgcac cggaataaga
     2101 tctttcacct ggatctgaaa ccggacaata tactcttgag gaaagagagg tccgacctgc
     2161 cttccctgat actgatcgat cttgactcgg cagccattgt ccgaccggga acccctttgt
     2221 tgtcgaccct gttgaagttg acagaactgt ggattcttca caaccacttc catcccatca
     2281 cgcacttgaa agaacgaata cggttcctta cggtctactc gggtaaagtg ctttctcgca
     2341 agtcggctcg aagaactcga ctattccttc tcaggtctcc agtgctgata catttgtata
     2401 aatgtcagcg gagaatgagt gtcatccata ccctcaggag attgctgcta tgcctgagag
     2461 tcctgagatg agtacacaac cttctatgtg ccctgacaca ccaggatcgg gagagagtac
     2521 cggtgactat ctcatacgtt atgtttggtc acagtcactt ccttgggaac cttcttgaca
     2581 tcattcatgc gcgtcaaggc cgcctctcgc agattgtcga gaatgtgccc gagcccctgc
     2641 atcctgagcg gccaccgctc gacaagcgcc tggcgagaat cccctaccct gtagaccgag
     2701 ttcgtct
//
LOCUS       DATMXI010000183         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_19383, whole genome shotgun sequence.
ACCESSION   DATMXI010000183 DATMXI010000000
VERSION     DATMXI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19383"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1316
                     /locus_tag="VN444_03435"
     CDS             309..1316
                     /locus_tag="VN444_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20897.1"
                     /translation="MTQHFTIHGIGVRVDTDIPLLATAIGELLRRFAHPPATDSQPLR
                     FIYERSGEAPDPLSNGSADGGGPLLFSTSWENAFDLAGRLGVNWDVYGRDGCLLLDYH
                     RHGRLRLDVSEGVLEGSLTEPLDLHPALLPSIFFFFPLAQLLARRGLHIIHAAALERN
                     GRGVLIPGLSGSGKSTSCVALMRGGYRCLSDDKPFLRETENSLELLAFPEMIDVTDQT
                     IAFFPELRERTAAIEVGYRKKRFCAETLYPGSTADAATPSVILFPQISGESTSRIEPL
                     SKIRALQALLPHSLLCFDREVSVRHFGLLSRLVETTACYRLYFGRDVMDLPRLIDPLL
                     G"
     gene            1343..>2235
                     /locus_tag="VN444_03440"
     CDS             1343..>2235
                     /locus_tag="VN444_03440"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWR20898.1"
                     /translation="MSMLQVSLSETSSDPAIVLSALDWSMLQRLVRHHRVGPLLSYSV
                     RRSRIRGIPDWLRSEWDSQRRETVATALYHVQALEELAGLFEARDIPFILLKGEALSK
                     TCYPDEGLRPYSDIDLLIKESSYEAARAALTAVGFCPRHPHLETEKRTLFGEVEFDRQ
                     GPRTLTVDLHWDTLMVSWGPPSLLSAAETWGLADQIQIGRQLVSVLGGETLLLHLCVH
                     FTFHHAFDGLILLCDLFLVLMREGGRIDWDRLLRKVDRYRCRYAVYYALTCVQSLLGA
                     HIPSPILKRLRPAIPVRLLMPV"
BASE COUNT          405 a          676 c          660 g          494 t
ORIGIN      
        1 accctatacg aaaccgactc tgaccaagca caagccgcta cgggatatca cggcgaccgg
       61 cgggtcacct agatagagag ggaagagcag ctatgaagcc aactccaaag cagccctaca
      121 cgaaaccaac gctgacgaag cacaagccgc tgcgggatat tacggcagca aatccctcga
      181 ttgcataagt ctttatcggt tgggaatatg cgtggatgtg acgacgcttg cctgtgaggt
      241 tgcgtcgcgt cgcccaccgc cgatgagggc ggcttggcat gcaaagtaat ggcactatga
      301 gcggtttctt gacgcagcat tttactattc acggcatcgg cgttcgggtg gacactgata
      361 ttccgttgtt ggccaccgcg attggtgagt tgctgcgccg gtttgcccat cctcccgcga
      421 cggacagtca gccgctgcgg ttcatctatg aacgtagcgg ggaggccccg gacccgcttt
      481 caaacggatc agccgacgga ggcgggccgt tgctcttctc gacctcctgg gaaaatgcct
      541 tcgatcttgc tggccggctc ggggtcaact gggacgtcta tggtcgggat ggttgtctgt
      601 tactggatta tcatcggcat ggccggctac ggcttgacgt ctcggaaggg gtactcgagg
      661 gatcgctcac tgagcctttg gatctccacc ctgcactgct ccccagcatt ttcttctttt
      721 ttccgctcgc gcagcttctg gcccggcggg gactccatat aattcatgca gccgcactgg
      781 aacggaacgg gcgtggggtg ctgatcccag gcttgagcgg gagcggcaag agcaccagtt
      841 gtgtggcgct catgcgcgga gggtatcgct gcctctctga tgacaaaccg tttctccggg
      901 agactgagaa cagccttgag ctgcttgcct tccccgagat gatcgatgtg accgatcaga
      961 ccatcgcctt ctttccggag cttcgtgaga gaacggccgc gatcgaggtg ggttatcgaa
     1021 agaagcggtt ctgtgccgag acgctctatc cgggctcaac ggccgatgcc gccacgccca
     1081 gcgtgatcct gtttccacag atcagtgggg agtcgacgag cagaatcgag ccgctttcaa
     1141 agatccgggc gcttcaggcg ctcctgcctc atagtctcct ctgctttgat cgcgaggtct
     1201 cggtgcgtca cttcgggttg ctctcacgcc tcgtcgagac gactgcctgc tatcgtctgt
     1261 attttggccg tgacgtcatg gacctgcccc gcctgattga tccgctcctg ggatgaaaag
     1321 ttccccagag gggcacgctc tcatgagcat gctccaggtc tcgctgtctg agacctcttc
     1381 cgatccggca atcgtcctct ccgctctcga ctggtccatg ctccagcgct tggtccgtca
     1441 ccaccgggtc ggaccgctcc tctcgtacag cgtgcgccgg tcccggattc gtgggatccc
     1501 agactggcta cgatcggaat gggactccca acgccgcgag acggtcgcta ccgccctgta
     1561 ccacgtgcag gctctagagg agctggcagg gctgttcgag gctcgtgaca tcccattcat
     1621 cctcctgaag ggcgaggccc tctcgaagac atgctatccg gatgaaggac tgcggcccta
     1681 cagcgacatc gatctcctca tcaaagagag ctcctacgag gcggcgaggg ccgcgctgac
     1741 agcggtcggg ttttgccctc ggcacccgca cctcgagacc gagaagcgca cgctattcgg
     1801 agaggtagag ttcgatcggc aaggtccaag aacgctgacg gtggatctcc attgggatac
     1861 cctcatggtc tcctgggggc cgccatccct gttgagcgct gcagagacat ggggcctcgc
     1921 agatcagatt cagatcggga ggcaactcgt atcggtactc ggaggcgaga ctctactgct
     1981 ccacctctgc gtccacttta cgttccacca tgcctttgac ggcctgatcc tcttgtgtga
     2041 tctgttcctg gtcctcatgc gggaaggtgg ccggattgac tgggatcgtc tcttgcggaa
     2101 ggttgatcgc tatcggtgcc gctatgcggt ctactatgcc ctgacttgtg tacagtcgct
     2161 cctgggggcc cacatcccgt ccccaatcct aaagcgactg aggcctgcga ttcccgttcg
     2221 gctcctcatg ccggt
//
LOCUS       DATMXI010000184         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_72308, whole genome shotgun sequence.
ACCESSION   DATMXI010000184 DATMXI010000000
VERSION     DATMXI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72308"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(601..1028)
                     /gene="rnpB"
                     /locus_tag="VN444_03445"
     ncRNA           complement(601..1028)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN444_03445"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1094..1954)
                     /locus_tag="VN444_03450"
     CDS             complement(1094..1954)
                     /locus_tag="VN444_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWR20899.1"
                     /translation="MQAWRLFSPGAVVIVVGIGMMVVSGCETNPYTGRSQLLMTSVSE
                     EMQLGAQAYSQVKNDPKLRQSQDPREIEPVKRIAARIIEAAKRSKYAEMAQQFQWEVT
                     VIKDDKTANAFALPGGKMAVYTGIFPMAKTEAGLAAVMGHEVVHALARHGAERMSQGQ
                     LTNVGLQVVGAAAGATGGGGTLGQAAMAALGVGAQVGVLLPFSRKHESEADYVGILLA
                     ADAGYDPREAVALWERMGQMSGGAPSEFLSTHPSHETRITQLKRWMPEAMAIYQTRPP
                     APVALLPSVQ"
     gene            2068..>2660
                     /gene="rnhC"
                     /locus_tag="VN444_03455"
     CDS             2068..>2660
                     /gene="rnhC"
                     /locus_tag="VN444_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HWR20900.1"
                     /translation="MTKTTEKTRIERIGIDESGKGDYFGPLVIAAVFVDATTQDELTL
                     MQVRDSKKISDGRILDMAPDIRMICPHSIIAIGPQRYNELYAKIHNLNRLLAWGHAKA
                     LETLLDRVSCERAIADQFGDERLILNALQEKGRTITLEQRHKAESDLAVAAASILARA
                     EFLQRLKRLSDEIGTTLPKGASPAVELAGRMIVKKHG"
BASE COUNT          586 a          831 c          661 g          582 t
ORIGIN      
        1 ccaggaagcg caaaaatcgt cttcacgcct tcccgtttga gacattcgat gaggatctca
       61 gcccctgtaa gcctcatggc agaacctccc tccataccac cggtgaaaac caggtagcgg
      121 agtcggcaca atctaggata acgtacagct cgtcaaagtt tagacgagat atagctctca
      181 cgattgttca gaacattctg aaaaatcagg gagaaagaga aagatggcta atcctatcac
      241 cacgcgtttc catgcgtcaa tagtggggcc ggtctgagag cctccttccc tgacagtcta
      301 ccttcgcaca tgatatcttc agcactccaa cacattggct cggatcgcgc ctctatgagc
      361 atgcctatcc gcgtaaccat tttatctcgt gaagattgcc atctctgcga tgtcgtctac
      421 cgtattgcag cacatcttca gtcagagctg cacattgaaa tgaacatcgt ggatgttgac
      481 agtgacagtt ccttaacaga acggtacggg acgagggttc ctgtcgtctt actcgacgag
      541 gtcgaacact tcgcagggag ggtgacggaa gaagaactgc gtcgagcgat aaaaaaagcg
      601 cggtggagaa gacctataag ccggattctg tcccgcctag gatttgctcc caaacggtga
      661 tgatcatttc tctgggattc gagttacctc gaacctcaag cgacctaccc gaagacctcg
      721 gccgggccag ccggtcgccg accctccgaa gaaagccgac agatgtcttc ctatttggcc
      781 ttgcatcggg cgacgcttac cctgccggtg atgtcgccac caccgcggtg ggctcttact
      841 ccaccgtttc acccttacct gagccaagct gacgggattg gaatcccatc gcttcgccca
      901 tcggcggtct attctctgtg gtgctggtgt cggatcactc cgcctgggaa ttgcccagcg
      961 ccctgcccat gaagtccgga ctttcctctt acagcgttac cgctccaagc gatcacccgg
     1021 tcttctccct cgcgccacag tactatagga ctgactgccc gccctttcaa gggctcatcc
     1081 agcatgcctc gacctactgc acagacggca acagcgctac gggcgctggg ggcctggtct
     1141 ggtaaatagc catggcttcc ggcatccatc ttttcagctg agtgatgcgg gtctcgtggc
     1201 ttggatgcgt agacagaaat tcagacggcg cgccgccgga catctgcccc atcctttccc
     1261 acagggctac agcctcgcgc gggtcgtacc ccgcgtctgc tgcgagcagt atccccacat
     1321 aatccgcctc cgattcgtgc tttcgactga agggcaatag tacaccgact tgtgccccga
     1381 cacctaacgc cgccatcgcc gcttgcccca acgtcccccc tccgcctgtg gcgccggctg
     1441 ccgcgccaac gacctgaagt cccacattcg tcagctggcc ctggctcatc cgttccgcac
     1501 catgccgagc cagcgcatgg acgacttcat gtcccatgac ggcggctaac cctgcttcag
     1561 tcttggccat gggaaaaata ccggtataga ccgccatctt gccgccgggc agcgcaaagg
     1621 catttgccgt cttgtcatcc ttgatcaccg tcacttccca ttgaaactgc tgtgccatct
     1681 cagcatattt ggatcgtttc gccgcctcta taatcctggc agcgatccgc ttgaccggtt
     1741 cgatctctcg cggatcttgc gactgccgga gcttgggatc gttcttcact tgactatagg
     1801 cctgtgcgcc taattgcatc tcctctgata ccgaagtcat caacaactgt gatcgccctg
     1861 tgtagggatt cgtttcgcac ccactgacca ccatcatccc gattcccacg acaattacca
     1921 ccgctccagg cgagaacaac ctccatgcct gcatcaccca tcctcctcaa taaagactcg
     1981 ctgtcttcga cttgagaaat tgaccgtccc ttgaggctct gttagattac cgggccgccc
     2041 ggagaatctc tactaagcca cagacctgtg accaaaacca ctgaaaaaac ccgcattgag
     2101 cgaatcggta ttgatgagtc cggcaaaggg gactattttg gtcccctggt catcgccgcc
     2161 gtctttgtcg acgcgacgac acaggatgaa ctgactctca tgcaggtgcg ggacagcaag
     2221 aaaatttccg acggccgcat tctcgacatg gcgcctgaca tccggatgat ttgcccgcat
     2281 agcattatcg cgatcggccc gcagcgatac aacgaactgt acgcgaagat ccacaacttg
     2341 aatcgcctgt tggcctgggg acatgccaaa gctctcgaga ctctcctaga cagggtttca
     2401 tgtgagcgcg caatagccga tcagttcggc gacgaacggt tgattctcaa cgcactacag
     2461 gaaaaggggc gcaccatcac tctggaacaa cggcataaag ccgagtccga cctggccgtg
     2521 gccgctgcgt cgatcctggc ccgtgcagaa ttccttcagc gattgaaacg tctctcggat
     2581 gagatcggca caacgttgcc caaaggcgca tcaccagccg ttgagctggc cggacgcatg
     2641 atcgtgaaga agcatggaga
//
LOCUS       DATMXI010000185         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_104677, whole genome shotgun sequence.
ACCESSION   DATMXI010000185 DATMXI010000000
VERSION     DATMXI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104677"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VN444_03460"
     CDS             complement(<1..909)
                     /locus_tag="VN444_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWR20901.1"
                     /translation="MAGRSLHLIDGSSYLFRAYHALPPLTNSKGVPTGAVYGFTKMLL
                     KIIRDERPEAMVVVFDSAGPTERHTQFADYKANRAQMPDDLSRQLPYIHRVVEAMRIP
                     LLMQQGQEADDLIGSLARQAAAQDCQVTIATSDKDMLQLVGPEIRIYDWMKEKVYNEA
                     EVQERFGVSPGQVVEMMGLMGDPIDNIPGVHGIGEKTARSLIQQFGSIEEMVSRLHEV
                     KSAKVREILRRQIEQARLSRDLARIMTDLPVRLDLGQAALQAPDYPALQALFRELEFA
                     ELQRAFTPATTRSSLRMVIIDREEEID"
     gene            complement(1068..2159)
                     /gene="dnaJ"
                     /locus_tag="VN444_03465"
     CDS             complement(1068..2159)
                     /gene="dnaJ"
                     /locus_tag="VN444_03465"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWR20902.1"
                     /translation="MSKRDYYQVLGVDQEAPPEEIKRAYRRLAHKYHPDKNSGDKASE
                     EQFKEATEAYEILSNPEKRAAYDRFGVAGEGAGFGGFGDARAGSVFEDLFEGFFGGSA
                     RRATSRGADLRYNLEISLEEAVIGTAKEITIPRLEPCGACKGSGAKPGTSPIACRSCR
                     GSGQVRYSQGFLTISQTCSACRGEGRVVEHRCRDCRGTGRSRSDRSLTVKIPAGVETG
                     MRLKLQGEGEAGPQWGDRGDLYVVITVKEHPLFSRHGDDLSCEVPVSFVQAALGAELE
                     VPSFFGMTKLKIPPGTQSGAEFRIRGKGVANVRGHGLGDLAVRIVVEVPRRLSIQQRE
                     LLEAYAALEGGDEGPLVTSFFEKVKSLFS"
     gene            complement(2246..2788)
                     /locus_tag="VN444_03470"
     CDS             complement(2246..2788)
                     /locus_tag="VN444_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWR20903.1"
                     /translation="MDQESEEAKVSTSDSDNTQESPVVSSTELESTISKLQAGLKEKT
                     TEIESLNDRLLRLQAEFENYKKRAARERSEFVRFANEGLLLDLLPVVDSLEHAATTTR
                     SGIDAQGVAEGLDVIRRLFQATLEKAAVKFIEALGHEFDPNFHQAVAQVESTDGRDNI
                     VVEEVRKGYLLKDVCCGRRW"
     gene            complement(2876..3916)
                     /gene="hrcA"
                     /locus_tag="VN444_03475"
     CDS             complement(2876..3916)
                     /gene="hrcA"
                     /locus_tag="VN444_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWR20904.1"
                     /translation="MRTHGLTPRECQVLKVIIHDYITSGEPVGSRSIARRHLGHLSSA
                     TIRNVMADLEEIGYLSQPHASAGRIPTDSGYRFYVDSLMQRPKLSRVEESRIEQGIRP
                     SHGEAEELVQGVSRILSDLSRYASVVLAPKFAQNTWRRINFVHLNRERILVVLMADSG
                     LVQQKVIAIDELIEQPELDRISNYLSSILGGVTLHEVRKRIIARMAEERDEFDRLMQR
                     ALELSNKTIEGEEEHVYIGGTANIAHQPEFADVKKMKNIFAAFEEKSKLVKILDYCLT
                     QEGLRIIIGGESEVREMRELSLIAAPYKSGDHAVGVLGIVGPKRIAYDRMAALVDCTA
                     RLVSKLLTGADV"
     gene            complement(4282..5442)
                     /gene="hemW"
                     /locus_tag="VN444_03480"
     CDS             complement(4282..5442)
                     /gene="hemW"
                     /locus_tag="VN444_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWR20905.1"
                     /translation="MDPGSSPDPKSIGLYIHIPYCLSRCHYCDFNTYRLDADHVQQYV
                     DGLAQEITLRASAMAIRDRQVCSVFFGGGTPSILQASQLIGILDHCRAAFTFEDGVEV
                     SLEANPGTVDLPKLRTLWEGGVTRLSVGVQAVQDRLLQRIGRAHTACEAKQAFWLMRE
                     AGFSNINLDVMFGLPGQSTGDWSETLDWAISVGPEHVSPYGLILEEGTLLHREHCQGE
                     IGLPDEETETAMYRMAVDRLLDAGFEHYEISNFARPGFRCRHNLVYWRHQEYLGIGAG
                     AHSFLSGCRFYNELLPASYVCAIGERGTAVAGNEELSAEMLRSERLMLGLRLRAGLDV
                     QTFKDVLGIEDLTASDRVARLLDDGLLRVKEGRVQITERGLLVANELIVQLL"
     gene            complement(5472..6110)
                     /gene="lepB"
                     /locus_tag="VN444_03485"
     CDS             complement(5472..6110)
                     /gene="lepB"
                     /locus_tag="VN444_03485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWR20906.1"
                     /translation="MTRQMRDETIKKDQEHTSQPGAKPEKSVIRQYAEAVIIAIILAL
                     VIRSFIVQAFKIPSGSMLQTLQIGDHILVNKFLYWFTDPQHGDIIVFKYPQDEGRDFI
                     KRVVALPGEKMEVREKQVYINDKPLAEPYTVHLDPAIIGDPGSSRDNFGPVMVAPGQL
                     FMMGDNRDYSMDSRFWGFLDMKKIKGKAFIVYWSWDHERFRPRWERIGMLVR"
BASE COUNT         1299 a         1921 c         1598 g         1318 t
ORIGIN      
        1 atcgatctcc tcctcgcgat caatgatgac catgcggagc gagctacgtg tagtcgcagg
       61 ggtaaacgcc ctttgaagtt ccgcaaactc cagctcccga aagagagcct gtagagcagg
      121 atagtcgggc gcctgcaggg ccgcctgccc caggtccagc ctcaccggaa gatctgtcat
      181 gatgcgggca agatcccggc tgaggcgggc ctgctcgatc tgacgtctca gaatctcccg
      241 gacctttgcg gacttgacct catgcaagcg actcaccatc tcttcgatgc tcccgaattg
      301 ctggatgagg ctcctggcgg tcttctcccc gatcccgtgc acgccaggga tattgtcgat
      361 cggatcgccc atcagtccca tcatctccac cacctgaccg ggggacacac cgaagcgctc
      421 ctgaacctct gcttcgttgt agaccttctc cttcatccaa tcatagatcc ggatctcggg
      481 cccaacgagt tggagcatgt ccttatcgct cgtagcaatg gttacttgac aatcctgcgc
      541 cgctgcctgt ctcgccagcg agccgatcag atcgtccgcc tcttgcccct gttgcatcag
      601 gagcgggatt cgcatcgcct ccaccacacg atggatgtag ggaagctgac ggctcagatc
      661 gtccggcatc tgcgcgcgat tggccttgta gtcagcgaat tgcgtatgtc gttcagtcgg
      721 tcccgcagaa tcgaagacca cgaccatggc ctccggacgc tcgtcccgga taatcttcag
      781 aagcatcttg gtgaaaccgt atacggctcc ggtcggaacc cccttgctat tcgtgagggg
      841 tggaagcgca tggtaggccc gaaacagata ggaactcccg tcaatgagat gcagcgatct
      901 acccgccatg gattagcatc cttaataaat agccgtcagc gatcagcgct cagtcatcag
      961 cttcggacaa ggcaggggac gcatcggaaa aagctgacag cttctctacc tctgtctagc
     1021 agcaatcgcg ggatatggaa tttgagagca gcatgcagca gggcagatta gctgaaaagg
     1081 ctcttgacct tctcaaagaa gctcgtgacc agagggccct cgtctccgcc ctccagtgca
     1141 gcgtaggcct caagcaactc gcgctgctgt atggagagcc tcctgggcac ctcgacgacg
     1201 attctgaccg caaggtctcc caggccatgg ccccgcacgt ttgccacgcc ctttccacgg
     1261 atgcggaact cagcgccgga ctgggttcct ggggggatct tgagctttgt catcccgaaa
     1321 aagctcggaa cctccagctc ggcccccaag gctgcctgaa caaagctgac ggggacctca
     1381 caggagaggt cgtcgccgtg ccgtgagaag agcggatgtt ccttcacggt gatgacgaca
     1441 tacaggtcgc ctcgatcccc ccactgtgga ccggcctcgc cctcgccttg aagcttcagg
     1501 cgcatcccag tttccacgcc cgcaggaatc ttcaccgtaa gggaccgatc ggaccgggac
     1561 cgccccgtgc ctcgacagtc ccggcatcga tgttcgacga cacgcccctc gcctcgacag
     1621 gccgagcagg tctggctgat cgtgagaaag ccttgtgagt accggacctg gccgctaccg
     1681 cggcaggaac gacaggcgat tggggacgtc ccaggcttcg cgccgctccc cttgcaggcg
     1741 ccgcagggct ccaaacgcgg gatggtgatc tctttcgccg tcccgatgac cgcttcctca
     1801 aggctgatct caaggttgta gcgaagatcg gcgcctcggg aggtagcccg tcgagcggac
     1861 cctccaaaga acccctcgaa gagatcctcg aacacagacc cagccctggc atcgccaaag
     1921 cccccgaagc cagccccttc gccggcaacc ccgaaccggt cataggccgc tcgtttctcg
     1981 ggattgctca ggatctcata ggcctccgtg gcctctttga actgctcctc cgacgcctta
     2041 tcgccagagt tcttgtccgg gtgatactta tgggccagcc gacggtaggc tcgtttaatc
     2101 tcctccggag gggcttcctg atccaccccc agcacctggt agtagtcgcg cttgctcatg
     2161 gctcacacgt ttcgagtttc gggttcatcc tcatcgacac tcgaagctcg aaactcagaa
     2221 ctcagaccct tcgcttttga taccttcacc atcgccggcc gcagcaaacg tccttcagga
     2281 ggtacccctt tcggacctcc tcgactacga tgttgtcccg gccatcagtc gactctacct
     2341 gagccacagc ctgatggaaa ttcggatcga actcatgccc caacgcctcg atgaacttca
     2401 ccgctgcctt ctccaatgtt gcctgaaaaa gccgacgaat gacatcgaga ccctcggcga
     2461 caccttgagc gtctatcccc gacctggtcg tggtggcagc atgctccaga ctgtctacga
     2521 cgggtagtag atccaacagt agcccttcgt tcgcgaacct cacgaactcg ctccgctcgc
     2581 gggccgctcg cttcttgtag ttctcaaact ccgcctgcag acgaaggagg cggtcgttaa
     2641 gcgactctat ctcggtagtc ttttccttga ggccggcctg cagcttgctg atcgtcgact
     2701 ctaactcggt cgaggagacg acagggctct cctgagtgtt atcgctatcg cttgtcgata
     2761 ctttggcttc ttcgctttct tgatccatta tctgatcgat tccctttcgt ccatcggtag
     2821 atccggcaga tcagtccgtc aggctacaca gcacaccatc actcttgaga acaggctaca
     2881 catcggctcc tgtcagaagc ttactgacga gcctcgcggt gcaatcgacg agggccgcca
     2941 tgcggtcgta ggcgatccgt ttcggtccca cgattccgag cacgccaacg gcatgatccc
     3001 cactcttgta cggcgcggca atcaggctga gctcgcgcat ctctctgacc tcactctctc
     3061 cgccgatgat aatccttaac ccctcctggg tcaggcagta atcaaggatt ttcaccagct
     3121 ttgatttttc ctcgaaggcc gcgaagatgt tcttcatctt ttttacgtcg gcaaactccg
     3181 gctgatgagc gatgttcgcc gtccctccaa tatagacgtg ctcttcctca ccttctatcg
     3241 tcttgttgct gagctctaga gcgcgctgca tcagacggtc gaactcatcc cgctcctcgg
     3301 ccatccgggc aatgatcctc ttcctcacct cgtgaagggt caccccaccc aatatactgc
     3361 tcaggtagtt agagatccga tccagctccg gctgctcgat cagctcatcg atagcgatca
     3421 ccttctgctg tacgagcccg gaatcagcca taaggactac caggatccgc tcccgattga
     3481 ggtggacaaa gttaatgcgt cgccaggtgt tctgagcaaa ctttggcgca aggacgacgg
     3541 aggcgtaccg tgatagatcg gagaggattc ggctgactcc ctgcaccaat tcctcggctt
     3601 caccatggct cggacggatc ccctgctcga ttcggctttc ctcgaccctc gataatttag
     3661 ggcgctgcat gaggctatca acgtagaagc ggtacccgga atcggttggg atcctgccgg
     3721 ctgaagcgtg cggttgagag agatagccta tctcctcaag atccgccata acattgcgga
     3781 tagtagcaga gctgaggtgg cccagatggc gtctggcgat acttctggat cctacgggct
     3841 ccccggaggt gatatagtca tggataatca ccttcaggac ttgacactcc cttggagtaa
     3901 gtccatgtgt ccgcatcagc cccctctgcc ccctttcctt gcccttcttt caacgcttgg
     3961 cactccccta aggagagcgc caagtgccta aaaaatacta aagacaaaca gccactcatg
     4021 tcaagcggat ttgccgtccc caagaacaga gattgacagc gtaacccgtt cattctcctg
     4081 tcatgacata ttcgtagggg cggggttgat ccccgcccgt tgatcgggag ggcataaagc
     4141 cctcccctac agtgcgaaca cccgacgggt gcgcggcact ctcactgcga ggagcgagat
     4201 tgcttcgcta cactcgcaat gacacatcgc aatctctatt cttggggccg tcggtcgcaa
     4261 gctagaaaac atgctataga atcagaggag ttggacaatc agttcattag ccacgaggag
     4321 cccccgctct gtgatctgca cccgcccctc cttcacgcga agaagcccat catccaggag
     4381 acgggcaact cgatcagacg cagtgagatc ttcaatgcca agtacgtcct tgaatgtctg
     4441 cacgtccagt ccggcccgga gccgaagccc cagcatcaga cgctcagatc gcagcatctc
     4501 agcggaaagc tcttcgttgc cggccacggc tgtcccacgc tcgccaatgg cgcacacgta
     4561 gcttgcaggc agtaattcgt tataaaaccg gcacccggaa aggaatgagt gagctcctgc
     4621 cccaatgccg aggtactcct ggtggcgcca gtaaaccaga ttgtggcgac aacggaagcc
     4681 gggacgcgca aagtttgaga tttcgtaatg ctcgaatccg gcgtcaagca gccggtctac
     4741 ggccatccgg tacatcgctg tctctgtctc ttcgtcgggt agcccaatct ccccttggca
     4801 atgctcccgg tgcagaagtg tcccctcttc gaggatcagc ccatagggag agacatgttc
     4861 aggacccaca ctgatggccc agtcaagggt ctccgaccag tcacccgtac tctgaccggg
     4921 caggccgaac atcacgtcca ggttgatgtt gctgaagccg gcttcacgca tcagccagaa
     4981 agcctgctta gcctcgcaag cagtatgagc acgaccgatc cgctggagga gtcgatcctg
     5041 aacggcttga actcctacgc ttagacgggt caccccgcct tcccacagcg tacgaagctt
     5101 cggaaggtcc accgtcccag gattggcctc gagactgacc tctacgccgt cctcgaaggt
     5161 gaaagcggct cggcagtgat cgaggatgcc gatgagttgg gacgcctgga gtattgatgg
     5221 cgtcccgccg ccaaagaaga cggagcagac ctgccgatct cggatcgcca tagcggaggc
     5281 gcggagcgtt atctcttgcg ccagcccatc cacatactgc tgtacgtgat cggcatcgag
     5341 acgatacgtg ttgaaatcgc agtaatgaca gcgtgacagg caataaggga tatggatgta
     5401 taaaccaata gatttcgggt caggagaaga tcctgggtcc aaaactcaaa gctccaaact
     5461 cgaaactgcc ttcatctgac caacatccca attcgctccc agcggggcct gaagcgctcg
     5521 tgatcccacg accagtagac aatgaaggct ttccccttaa tcttcttcat gtcaagaaag
     5581 ccccagaacc tgctatccat actgtagtcc cggttatctc ccatcataaa gagttgaccc
     5641 ggcgcaacca tgaccggacc gaaattgtcc cgagacgaac ccggatcccc gatgatggcg
     5701 ggatccaggt gaaccgtgta cggctcagcg agaggcttat cgttgatgta gacctgtttt
     5761 tcccgtacct ccatcttctc gccaggcagg gcaaccactc gcttaataaa gtctctcccc
     5821 tcatcctggg gatacttgaa cacgataatg tccccatgct gcggatcggt aaaccaatac
     5881 aggaacttat tgaccaggat gtgatcaccg atctgcagcg tctggagcat tgatccagaa
     5941 gggattttga aggcttggac tataaagctc ctgatcacca gcgccaggat aatggcaatg
     6001 atgacggcct cggcgtactg gcggatgact gacttctccg gtttcgcgcc tggctgcgaa
     6061 gtgtgctcct ggtctttctt tatcgtctca tccctcatct gccgcgtcat gggcttttca
     6121 ccttcaggac cgccat
//
LOCUS       DATMXI010000186         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_56020, whole genome shotgun sequence.
ACCESSION   DATMXI010000186 DATMXI010000000
VERSION     DATMXI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56020"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN444_03490"
     CDS             <1..460
                     /locus_tag="VN444_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20907.1"
                     /translation="TGYSPANPPNTPAPNLIFSEDVEEADTHGAAVVHHGRFLWVADR
                     GRNFLFVVDTSRDVIVNRINLIGDASNDPTPDLLFPSPQGTRVYMSLRGPNPLTADPH
                     VSTGSTPGVGVIKVKRGGRHGDFFGIAPVSNVDASGIERADVHALAVRRR"
     gene            778..1209
                     /locus_tag="VN444_03495"
     CDS             778..1209
                     /locus_tag="VN444_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HWR20908.1"
                     /translation="MNVSKNTSRPELTLVLLLLVAGLSACAASDAGQARVFFTSPSDG
                     ATVTSPVKVAMGAENFIVEPAGTVRAGAGHLHIMVDANCVPAGQVVPFDAAHLHYGKG
                     QLEAELALSPGTHTLCLQAADGAHIALAGAGMTQTITLTVK"
     gene            1406..1957
                     /locus_tag="VN444_03500"
     CDS             1406..1957
                     /locus_tag="VN444_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20909.1"
                     /translation="MGEFEARPIDSFLVWSAAGYDNWRMIMGIIRCGVSSIIALFVVV
                     GSALFAFEAALAEEPVLESYTIGHGGNLGARPDGVAATLTDKFLGNGTAKARIKLKAK
                     EDSGTLSLRVSRSVGRGKITFALIVERIEVRGYDAAGALVYVRDLGGFVFGDSKSGKW
                     EEKIEDLPLEAQQVRIIFIGNYA"
BASE COUNT          497 a          673 c          753 g          485 t
ORIGIN      
        1 gactgggtac tcgcccgcta atccacccaa tacgccagcc cccaacttga tcttcagtga
       61 agacgtcgag gaggccgaca cccacggtgc ggcagtcgtc catcatgggc ggttcctttg
      121 ggtggccgac cggggccgta actttctgtt cgtggttgat acgtcgaggg acgtgatcgt
      181 gaaccgtatc aatctgattg gcgatgcgag caatgacccg actcctgatc tcctcttccc
      241 gtcgccgcag ggaacccgtg tctatatgtc gctacgcggg ccgaacccgc tcacggcaga
      301 tccccacgtc agcacgggaa gcacgcccgg ggtcggcgta attaaggtga agcgtggcgg
      361 gcgtcacggc gacttcttcg ggatcgcgcc cgtgagtaac gtcgacgcct caggtattga
      421 gcgagcggac gtacacgctc tggcggtgcg caggaggtag cgaatatcac ccacggctgt
      481 gcgcatgaaa ttcccaaggt gaccgcctaa ccatcgcatc aactcagact gccaactacg
      541 ccgcgcttcg ttggcagccg gttatgcggc acgttctccc tgcgcttcac tcatggagaa
      601 cacggggctg agccggtgcc ccttccctcc gctccgctcc gggagaacca ggggcacgaa
      661 accgaccgct tcgcggcggc tcagccccgt gttgggccgc cgacaagtgt cacacgacct
      721 taatgagcca ttcgccagcg ccaggagtgg ctcattgggc catccgcgag gtgtatcatg
      781 aacgtttcaa aaaacacgtc tcgaccggaa ctgaccttgg tgctgctcct gctcgtggcc
      841 ggactctcgg cctgcgcggc atcggacgcc ggccaagccc gcgtcttctt tacctcgccg
      901 agcgatgggg cgaccgtcac cagcccagtg aaggtggcaa tgggcgcaga gaactttatc
      961 gtcgaacccg ccggtacagt tagagccgga gcgggtcatc tccacatcat ggtggatgcc
     1021 aactgcgtac cagccggcca agtggtgccg ttcgatgccg cccacctgca ctacggtaag
     1081 ggtcagttgg aagctgaatt ggctttatcg cccgggacac acaccctgtg tttacaggcc
     1141 gccgatgggg cacatattgc actggcaggt gccggtatga cgcagacaat taccctcacc
     1201 gtgaagtgac atgcatggta gtcagagcgg ccggtgagaa gcgataagcc gaccgaaccg
     1261 cgttcagcgg gaggaatctg tcacagcccc ttgggagaca ggaagcccga agggctgtcc
     1321 tgtttccagg ggatcctccc gtagcgtctg aggtaagtcg tgatgggggc gacgacaagc
     1381 ggttgctgcg cgttgaaccg tagcggtggg agagttcgaa gctcgaccga tcgattcctt
     1441 tttggtgtgg agcgcagcgg gatacgataa ttggaggatg atcatgggta taataagatg
     1501 tggggtcagt tccatcatcg cgctcttcgt agtagtcggg tctgctcttt tcgccttcga
     1561 ggctgcattg gccgaggagc cagtactcga gagttacact atcggtcatg ggggaaacct
     1621 tggcgcgcgc cctgatggcg tcgcggcgac actcacagat aagtttctag gaaacggtac
     1681 ggcaaaggct cgtatcaagc tcaaagccaa ggaggattct ggcaccttat ctctgagagt
     1741 ctcaaggagt gttggtcgag ggaaaataac ctttgcgttg atagtagaac gtatcgaggt
     1801 ccggggctac gacgcggctg gcgcgctcgt ttacgtccgt gatctgggag ggtttgtatt
     1861 tggggatagc aagtcgggaa agtgggagga gaagatagaa gacctgccgc tggaagctca
     1921 acaggtcagg ataatcttta tcgggaatta cgcatgactg ttgcgccaga gccttgcctg
     1981 taaacgatca ggatggacac gagcggcacg gtcgttttcg actccttggt cagggtctac
     2041 aacgtccacg atcccccggg gttactgctg cctcgggcca tactgcgcgg tgctcagcgt
     2101 gaaagcgcga ggaagctgca cagccctttg gggtacggtg agccccaaag ggctgtcctg
     2161 tttccagggg ccctcccccc ccggagcgtc tgaagcacga gatggtgagt acaacagtga
     2221 acgatagcca cgtgttgaag cgtggcggga gggaggggga ccacggtcca gccgatcaac
     2281 tgggcttcgg aaaataggga cagacactat tatcgtgaca atttgtagta tttgcagtca
     2341 tgatcgtgca taccacggat tgctcgaatc gttgcgcccg gccacccgca tcatgtgacg
     2401 caacgagg
//
LOCUS       DATMXI010000187         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_4312, whole genome shotgun sequence.
ACCESSION   DATMXI010000187 DATMXI010000000
VERSION     DATMXI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4312"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..576)
                     /locus_tag="VN444_03505"
     CDS             complement(205..576)
                     /locus_tag="VN444_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20910.1"
                     /translation="MARALMDKTEAQQLANHTVFSGVKPGSICTRCGGLMVDDFYMDI
                     LNNVGEPKFAAQRCVQCGEVVDPVILRNRGTKLEPVTAQAAWKMTPSNHVTNVHDRFA
                     LQTHGTGFRCARGLESQAPQK"
     gene            715..1632
                     /locus_tag="VN444_03510"
     CDS             715..1632
                     /locus_tag="VN444_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWR20911.1"
                     /translation="MNTHRIHRVAKLTGLSKDVIRVWERRFGILQPTRGANRYRNYSD
                     EDVALLRFLKEQLDAGGSIGELAKLGREELLGRARASAPRVSFVDNTFSRLLGELLSA
                     LNPFNRVIFEKRLNGAVAVVPFEEALHGILLPLQEQVGQLWHESQIDVSIEHYVTKHI
                     QQKIFSAMNQLPVAEFGAKVVVACPPGEEHDIAALTVTYRCRVRGCRVYHLGANVPVA
                     SLANLCGRVEPDLTIMSFPLALSDDMATELIQALADEVSPVSNLAVGGHGAIAMRDVF
                     ERSHIEVLEDFTALDHKLDRLMRQPLSRE"
     gene            1651..2061
                     /locus_tag="VN444_03515"
     CDS             1651..2061
                     /locus_tag="VN444_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HWR20912.1"
                     /translation="MAQYPLTVFFDGACPICDREIALMKRLDRRRQLEFCDFSAQEYD
                     AASIGFTAADLATVIHARWADGRVITGVEVFRAMWEAVGLRFLAKLSRLSVVEPLVLK
                     AYAWFARNRLRLTGRSHACTEDTCRSALSSRRNR"
BASE COUNT          469 a          572 c          585 g          524 t
ORIGIN      
        1 accgttgaga gttccattta tacgcgtcac cttcttctgt ggctcaccga gcagataggc
       61 ccttccgggg tgtagagagg ggcctatcta cctcagcggg aggcgccgtg cggagtcgca
      121 gcgcccgcca agactgggaa aagaaaccgg cctccgtatc tctgctgtgg tcatctagtc
      181 tcatcgcacc accacgttga ccgtctattt ctgcggggcc tggctttcga gcccccgtgc
      241 acaacgaaag cctgtcccat gggtctgcag cgcgaatctg tcatgcacgt tcgtcacatg
      301 attgcttggc gtcatcttcc atgcagcctg agctgtcacc ggctctagtt tagttcctcg
      361 attacgcaga atgaccggat cgacaacctc accacattgc acacagcgct gggcagcaaa
      421 tttcggctca ccgacgttat taagtatgtc catgtagaag tcatccacca tgagtccgcc
      481 gcacctggtg catatcgatc caggtttgac cccagaaaac actgtatgat ttgccagttg
      541 ttgagcttcg gttttatcca ttaatgctct tgccataatg gcctccctag tttttctgtt
      601 gactgtttgt agcgactatg tctaatgtat aaaccatctt gtccaatgtg tcaagtgtta
      661 tatggcatat tatttgacaa acattaaaca tcgtaataga atgacatcac aaacatgaat
      721 actcatagaa ttcatagagt tgcaaaactc acaggtctct cgaaagatgt catccgggta
      781 tgggagcgcc ggtttggaat cctgcaaccg accagaggtg ccaatcgtta tcgtaattat
      841 tccgatgagg atgttgcgtt gctgaggttc ctgaaggagc agttggatgc tggtggttcg
      901 atcggagagc tggcgaaatt ggggcgcgag gagctattgg ggcgagcacg agctagtgcg
      961 ccgcgtgtgt cgttcgtcga caatacattc agtaggctct tgggggaact tctgtccgcc
     1021 ctgaatccct tcaatcgtgt gatctttgaa aagcgtttga acggcgccgt tgcggtcgtt
     1081 ccattcgagg aagcgcttca cgggatcttg ctcccgctcc aggaacaggt agggcagctg
     1141 tggcacgaga gtcaaataga cgtgtccatt gagcattacg ttacgaagca cattcaacaa
     1201 aaaatattct cagccatgaa tcagctgcca gtggccgagt tcggagccaa agtcgtcgtg
     1261 gcctgcccgc ccggtgagga acacgacatt gccgcgttga cggttaccta tcgatgtcgt
     1321 gtccgcggct gccgagtcta ccatttggga gccaacgtgc ccgtagcgtc gctggccaat
     1381 ctctgcggca gggttgagcc agacctgacg atcatgtcat ttcctctcgc actgtctgat
     1441 gacatggcca cggaactgat ccaggccctc gcggacgaag tgagtcccgt ctccaatctc
     1501 gctgtcggcg gacatggggc gatcgccatg cgggatgttt tcgagagatc tcacattgag
     1561 gtcttggagg acttcactgc gctggaccac aagctcgatc gattgatgcg gcagccctta
     1621 tcccgggagt agcaaggaga ggccgatcaa atggcacaat atccgctcac cgtattcttc
     1681 gacggagcct gcccgatttg cgatcgagag attgcgctca tgaaacggtt agaccggcgg
     1741 cgacagttgg aattctgcga cttctcagcc caggaatatg atgcggcatc catcggcttt
     1801 accgcagcgg atctagccac agtgattcat gcccgttggg ctgacgggag ggtgatcacc
     1861 ggcgtcgagg tgtttcgagc catgtgggag gcagtcggcc tgagattctt ggctaagctc
     1921 agccgtcttt ctgtagttga gccacttgtc ctgaaggctt atgcctggtt cgcacgtaac
     1981 cgcttgcggc tcaccggtcg ctcgcacgcg tgtactgaag acacatgcag atcagctctt
     2041 tcaagtcggc gcaatcggtg acctgattac caccattcac gcaacaaact ctgtgtgatc
     2101 gcgaccgata gagagtcata atgagtgcct atcctcggag ccaacagagt
//
LOCUS       DATMXI010000188         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_133745, whole genome shotgun sequence.
ACCESSION   DATMXI010000188 DATMXI010000000
VERSION     DATMXI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133745"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VN444_03520"
     CDS             complement(<1..1421)
                     /locus_tag="VN444_03520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWR20913.1"
                     /translation="MDCSNSKKRFSEKEYSCDELQDSQEKQGVRGGYIARFGQLSRVT
                     FYVLRLTATMQALLDSLKAKFPGAILAIRVDAARAETSVSVAATGLLEIARYLHDAPE
                     AAFDHLTDICSVDYPEDQLRFEVVYHLHSLPLQQRLRLKARITEDDPTIASVTGVWKG
                     ADFLEREVYDMMGIHFSGHPDLRRILMPEDYTEGYPLRKDFPTEGRGWRSQFDFIPRF
                     DEASVDVIESEISDAQKSVFRAEPDVPSSQRKEELLLNMGPQHPSTHGVLRVVLLLDG
                     ERVVKATPDLGYLHRGVEKLSEGLTYMQIIPHTDRLDYVCAMSNNYAYVRAVEKLLEI
                     EVPIRAEYIRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFRERETLLDMFEKLCG
                     ARLTLNYYRIGGVDSDFIPELVQQMKAFLETFPEKVKEYDSLIAANRIWIGRTKNVAV
                     LSAEDAINFGCTGPVLRGSGVAY"
     gene            complement(1366..1845)
                     /locus_tag="VN444_03525"
     CDS             complement(1366..1845)
                     /locus_tag="VN444_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWR20914.1"
                     /translation="MSFLERQFEANILTTNLDAVVNWSRKSALWPMTFGLACCAIEMI
                     ATVSSRYDLDRFGAGVFRASPRQADLMIIAGTVTRKMAPVIRRIYDQMPEPRYVISMG
                     SCATSGNHYNSYAVVQGVDQIIPVDVYVPGCPPRPEALLDGLLKLQEKIQRERVFVR"
     gene            complement(1851..>2042)
                     /locus_tag="VN444_03530"
     CDS             complement(1851..>2042)
                     /locus_tag="VN444_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20915.1"
                     /translation="CSCLSPFYWPGSGMPGRRGRWNGIEQGTVRMPYVLAERAASEGP
                     RSTRAVKGIPTIPFQLLVE"
BASE COUNT          468 a          603 c          558 g          413 t
ORIGIN      
        1 tcgtaggcga cgcccgaccc gcgtaagacc ggtccggtac agccgaaatt gatcgcatct
       61 tcggctgaga gcacagccac gttcttcgta cgaccgatcc agatgcggtt cgccgcgatg
      121 agcgaatcgt actccttaac cttctcggga aaggtctcga ggaacgcctt catctgttgg
      181 accagttcag ggataaaatc gctatcgaca ccgccgatcc gatagtagtt caaggtcagc
      241 cgggccccgc agagtttttc gaacatgtcc aacagtgttt cgcgctcccg gaatgtccaa
      301 aagaacacgg tcatcgcgcc gatatcgagg gcctgcgtac cgagccaaaa gaggtgcccg
      361 atgatgcgtt gcatctcagc cacgatggtc cggatatatt cagcccggat cggcacctcg
      421 atctcgagga gtttttctac ggcacgcacg tacgcatagt tgttggacat ggcacagaca
      481 tagtcgagcc ggtccgtgtg cgggatgatc tgcatgtagg tcaatccctc tgacaatttc
      541 tcgacacccc gatgcaggta gccgaggtct ggcgtggcct tcacaacccg ttcgccgtcc
      601 aataagagca cgacacgcaa cacaccatgc gtactcggat gctgtggccc catgttcagc
      661 agtaattcct ctttccgctg ggaactgggc acatcgggtt cagcacgaaa gacgcttttc
      721 tgggcgtcgg agatttcgct ctcgatgaca tccaccgagg cctcatcgaa tctcggaatg
      781 aagtcgaact ggctgcgcca gccacgccct tcggtcggaa agtcctttct caacgggtaa
      841 ccctctgtat aatcctccgg catcagaatg cgccggagat cgggatgacc ggaaaaatga
      901 atacccatca tgtcgtacac ttcacgttcc agaaaatcgg cacccttcca gactccagtg
      961 accgaggcaa tcgtcggatc atcctcggta atacgcgcct tgagacgaag acgttgctga
     1021 aggggaagcg agtgcaagtg gtagacgact tcgaatcgca gctgatcttc cggatagtcg
     1081 accgagcaaa tatcggtgag atgatcgaag gcggcctccg gcgcatcgtg caagtagcga
     1141 gcaatctcca gcagacccgt agcggcaaca gagacggagg tctcggcacg agcagcatcc
     1201 acgcggatcg cgaggatcgc acctggaaat ttcgccttca ggctgtcgag cagagcttgc
     1261 atagtcgccg ttaaacgtaa gacgtaaaat gtgacacgtg agagctgtcc gaaccgtgcg
     1321 atgtagccgc cccgcacacc ttgcttctct tgcgaatctt gtagctcatc gcacgaatac
     1381 tctttctcgc tgaatctttt cttggagttt gagcagtcca tcgagcaacg cttccgggcg
     1441 gggcggacag cctggcacat agacatcgac cggaataatc tggtccacgc cctggacaac
     1501 ggcgtaacta ttatagtggt tacccgaggt ggcgcaggaa cccatagaaa tgacgtaccg
     1561 cggttccggc atctggtcgt agatgcggcg gatcaccggc gccatcttgc gcgtcaccgt
     1621 gccggcgatg atcatgagat cggcttgccg cggggatgct cgaaagacac cggcgccgaa
     1681 acgatccagg tcgtaccgcg acgagaccgt ggcaatcatc tcgatcgcac agcaagcgag
     1741 gccgaacgtc atcggccata aggccgactt ccgagaccaa ttcacgacgg cgtccagatt
     1801 ggtcgtcagg atattcgcct caaactgtcg ttctagaaaa ctcatctctt ctactccacc
     1861 aatagctgga aggggattgt cgggatgccc ttcactgcgc gcgtcgaacg agggccttct
     1921 gaggccgcgc gttccgcgag cacgtagggc atcctgacag tcccctgctc aatcccattc
     1981 cagcgccccc ttcttccagg cataccagag cccggccaat agaatggcga taaacacgag
     2041 ca
//
LOCUS       DATMXI010000189         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_152566, whole genome shotgun sequence.
ACCESSION   DATMXI010000189 DATMXI010000000
VERSION     DATMXI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152566"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1486)
                     /locus_tag="VN444_03535"
     CDS             complement(14..1486)
                     /locus_tag="VN444_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWR20916.1"
                     /translation="MGDTTVEKRRIVQELAAIVGREWVISDPVELAVYECDGMTYLEK
                     ALPDVVVLPDGTEQVAEVVKLCDREQLPFLPRGAGTGLSGGAIAVQGGVIIGLNRMNR
                     ILEIDLANQRAVVEPGLVNIAMTQAVVDRGYYFAPDPASQSASSIGGNVAENAGGPHC
                     LKYGTTTMHILGLELVLASGEIVQVGGKALDCPGYDLTGLFVGSEGTLGIATKVTVRL
                     LRQAEAVKTLLASFRTIDAASEVVSEIIATGIIPSALEMMDRHIIKALEEWLQIGYPE
                     GAGAVLLIELDGPTAEIEAHAKRIERICAQHGVLDLGIAKDEQERTLLWKGRKGAVAA
                     VGRITHEFYLQDGVIPRTTLPQVLRDVEAISERNALVIANVFHAGDGNLHPLICFDSR
                     REGELQRAIAAGAEIMGLCLSVGGSITGEHGIGMEKIDFVPLMYSPDDLEAMRRVRVA
                     FDPSLRCNPGKLLPATRSCSETNVAYRPHRVEREGLSQRY"
     gene            complement(1518..2879)
                     /locus_tag="VN444_03540"
     CDS             complement(1518..2879)
                     /locus_tag="VN444_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWR20917.1"
                     /translation="MNSERLEGRLAGIVGVDHVVAAESCAPYAVDDKAPKAVVFPADA
                     AELSEVMKLASSEQLSVVPWGSGTKISLGGIPARVDLVVGLKRLNQIIDYEPGDLTAT
                     FQAGILLREAQTFLHSSGQFLPFDPAHCERATIGGILATNSSGPWRHRYGTARDLVIG
                     IRVVHADGTLTKGGAKVVKSVSGYDMNKLYVGSLGTLGIILEATLRLYPLPAVERTWI
                     ALFLTGEAAACAVAQILHSTIVCTRVELLSSFAAQSVGRQAGCTLPADTVAVAVSVGS
                     MSEAVDTQIAAIRKFCYQEGGVAGFLVEGSAQESLWRAICDFPSASGDGWSWATLKAS
                     VPLTKVLETIHRAEALGRDLGLESTAVSDAGCGIVRLYWRGETGSLERDPALVAKRVE
                     DLRSWVVLNGGSLVILSAPPAIKAKVDVWGPVGNALFLMQELKQRFDPQGLLNPGRFV
                     GGI"
     gene            complement(2993..>3896)
                     /locus_tag="VN444_03545"
     CDS             complement(2993..>3896)
                     /locus_tag="VN444_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWR20918.1"
                     /translation="GWHDYAAVAVRPPFDRPVSAGVPQGVLDEILICPPNDWDAFTAL
                     ASGRDDIAGVILEPGGGSCGTISTDHDFLTRLREFTRAQGVVLIFDEVISGFRLAPGG
                     AQQRYGIYADLTTLAKILAGGLPGGAVVGSAPLMDQLAFRDDPTWNREERVGHAGTFN
                     ANPLSAAAGLTTLQLLADGQTQIEADRAGQTLRNGLTEVIKALGADACVYGESSIVNL
                     FLGPHRHGLVLDRPSCKPDHHILMDHPNQEAYHRLRCALILHGVDFPLFHGWISAAHS
                     DEDVEKTIRAFEAALRLMQDEGAL"
BASE COUNT          785 a         1303 c         1096 g          712 t
ORIGIN      
        1 ggccagaagg aggtcagtag cgctgggaga ggccctcccg ttcaacacgg tgtggccgat
       61 aggcaacatt ggtttcgctg caggacctgg tggcgggcaa gagcttgccg gggttacacc
      121 gcaaagaggg gtcgaaagct acccgaactc tccgcatcgc ctcgagatcg tccgggctat
      181 acatgagcgg gacgaagtca atcttctcca tcccgatccc atgctccccc gtgatgctac
      241 cgccgacgga caaacagagc cccatgatct ccgccccagc cgcaatggcc ctctgcaact
      301 cgccttccct gcgggagtca aagcagatca gaggatggag gttcccatca cctgcgtgga
      361 agacattcgc gatcactaaa gcgttgcgct ccgagatcgc ttcgacatcc ctgagcacct
      421 gaggaagtgt ggtccgaggg atcacgccat cctgcaggta gaattcgtgg gtgatccggc
      481 ctacggccgc aaccgcgccc ttccgcccct tccagaggag tgtccgctcc tgctcgtcct
      541 tcgcaatgcc aaggtcaagg acaccatgtt gggcgcaaat ccgctcgatc cttttagcat
      601 gggcctcgat ctctgcggtc ggcccatcca actcaatcag cagtaccgcg ccggcgccct
      661 cggggtagcc gatctgcaac cactcctcaa gcgccttgat gatgtgacga tccatcatct
      721 ccagggctga ggggataatg cccgtggcaa tgatctccga caccacctcg ctggccgcgt
      781 cgatggtgcg gaatgaggcc agcagcgttt ttacggcctc ggcctggcgg aggagacgaa
      841 cggtcacctt cgtggcgatc cccaaggttc cttccgagcc gacgaagagg ccggtaaggt
      901 cgtagccggg gcagtccagc gccttaccgc caacctgcac aatctctccc gatgccagga
      961 ccagttccag tccgaggata tgcatcgtcg tggtcccgta cttcaggcag tgggggcctc
     1021 cggcattctc cgccacgttg ccgccgatgc tggaggcgga ctgactcgct ggatccggag
     1081 caaagtaata cccacggtca acaacggcct gggtcatggc gatgttgacc aggcccggct
     1141 ctaccacagc tcgctgatta gcaagatcga tctccagaat cctgttcatg cggttcagcc
     1201 cgatgatcac cccgccctga acagcaatgg cgccacccga cagcccggtc cccgctccac
     1261 gcggcagaaa gggaagttgc tccctgtcac agagcttcac cacctcggcc acctgctctg
     1321 tgccgtctgg aaggaccaca acatcaggca gggccttctc caggtaagtc atcccgtcgc
     1381 actcgtagac tgccagctca accggatccg agatgaccca ttcccggccc acgatggctg
     1441 ccagttcctg cacaatcctc cgcttttcca cagtcgtatc ccccataccc tatcccctaa
     1501 accctatacc ctttccccta gatgcctccc acgaagcgac caggattaag gagcccctgg
     1561 ggatcgaacc gctgcttcaa ttcctgcatc aggaacaggg cgtttccaac cggaccccac
     1621 acatcgacct tagccttgat cgccggcgga gccgacagga tcaccaggct tccaccattg
     1681 agcacaaccc aactccgaag atcctcgacc ctcttcgcga caagggccgg atccctctct
     1741 agcgaacccg tctcacctct ccaatacagt cggacgatcc cgcagccggc atcagagacg
     1801 gcggtcgact ccaggccgag gtctcggcca agcgcttcag cgcggtggat ggtctcaagg
     1861 acctttgtca gcgggacact cgccttcagc gtcgcccaac tccatccatc gccgctcgct
     1921 gacggaaagt cgcagatggc tctccacagg ctttcttgcg cgctcccctc tacgaggaat
     1981 ccagccacgc ccccttcctg gtagcagaac ttcctgatgg ccgcgatctg ggtatccacc
     2041 gcctctgaca tacttcccac tgacaccgcc accgcgacag tgtccgccgg aagagtacag
     2101 ccggcctgcc tgccaaccga ttgcgccgca aaggacgaga ggagttcgac tctggtgcag
     2161 acgatggttg agtggaggat ctgagctacc gcacacgcag ccgcctcacc cgtcaggaac
     2221 aacgctatcc aggttcgctc cacggccggc agtggataga gcctcaaggt ggcctcgaga
     2281 ataatcccaa gagtccctag cgagccgacg tagagtttgt tcatgtcgta cccgctgacg
     2341 ctcttgacca ccttcgcgcc gcccttcgtg agagtgccat cggcatggac aacgcgaatc
     2401 ccgatgacca ggtcgcgggc ggtcccgtac cgatgccgcc acggaccgct cgaattggtg
     2461 gcgaggatac ctccaatcgt tgcccgctcg cagtgcgctg gatcgaaggg tagaaactgg
     2521 ccgctgctat ggagaaacgt ctgagcctcg cgaagcagta tcccggcctg aaaggtcgcc
     2581 gtaagatcgc ccggttcgta atcgatgatc tgattgagcc gctttagccc aacaaccaga
     2641 tcgactctcg caggaatgcc cccaaggctg atcttcgttc cgctccccca cggcacaacc
     2701 gaaagctgct cggaggacgc gagcttcatg acctcggaga gctcagccgc atccgcagga
     2761 aagacaaccg cctttggagc cttgtcgtct acggcgtatg gggcacagga ctccgccgct
     2821 acgacatggt caactccgac gatcccagcc agccgtcctt ccaggcgttc agagttcatg
     2881 gttcatggtt ttcggtccac cgtttacggt tgacggttca cagtgcgggg acacacttga
     2941 ctcctgcaat ggtgaatccg tctatgaacc cttaaccctg aactatcaac tcctacaggg
     3001 caccctcgtc ctgcatcagg cggagcgcag cctcgaaggc tcggatcgtc ttctcgacgt
     3061 cctcatccga gtgcgccgct gagatccacc cgtggaagag ggggaaatcg acgccgtgca
     3121 ggatcagcgc gcaccgtagc cggtgatagg cctcctggtt cggatggtcc atgaggatgt
     3181 ggtggtcagg cttgcaggag ggccggtcga ggacaaggcc atgacgatga ggcccgagga
     3241 agagattgac gattgaagat tcaccgtaga cacaggcatc cgccccaagc gccttgatga
     3301 cctcggtaag cccgttgcgc aacgtctgtc cggcacgatc ggcctcgatc tgggtctgtc
     3361 cgtcagccag aagctggagc gtcgtcagac cagccgccgc tgagagcgga ttcgcgttga
     3421 aggtcccggc atggcccacc cgctcttccc ggttccatgt cggatcgtcc ctgaaggcca
     3481 actggtccat cagaggggcg ctgccgacta cggccccacc agggagcccc ccagccagga
     3541 tcttggccag cgtggtcagg tctgcgtaaa tcccataccg ctgctgcgcg ccaccagggg
     3601 ccaaacggaa cccgctaatc acctcgtcga agatgagaac cacgccttgg gctctggtga
     3661 attcccggag ccgggtcagg aagtcgtggt cggtcgagat cgtgccgcaa ctgccacccc
     3721 cgggctccag tattacaccg gcgatatcgt ccctgccgga ggccagagcc gtgaacgcgt
     3781 cccagtcgtt cggtgggcaa atgaggatct cgtcaaggac gccttgcggt acgccagcgg
     3841 acacaggccg gtcaaagggc ggacggaccg cgaccgccgc atagtcgtgc catccg
//
LOCUS       DATMXI010000190         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_265639, whole genome shotgun sequence.
ACCESSION   DATMXI010000190 DATMXI010000000
VERSION     DATMXI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265639"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            585..1496
                     /gene="rsmH"
                     /locus_tag="VN444_03550"
     CDS             585..1496
                     /gene="rsmH"
                     /locus_tag="VN444_03550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWR20919.1"
                     /translation="MEAPRHIPVMADEVLFWLVHEGARIYLDCTVGYSGHAEKLLEAT
                     GPDSRLIGLDRDAAAIAASRDRLARFGDRVRLLYGHFMGLKQHLAASGIGRVDGILFD
                     LGVSSAQLEEPTRGFSFQGDSLLDMRMDQSMSGTAADLVNRWPEAQLADTIFQFGEER
                     FSRRIARAIVHARERHPLATTKELVSVIEGAVPANYRHGRIHCATRTFQAFRIAVNQE
                     LDCLEPALRDAVDVLSPGGRLCVISFHSLEDRIVKHTFRALSGKNDPALWVLTKKPQV
                     PTREESNRNPRSRSAKLRAAQRISREG"
     gene            1499..1834
                     /locus_tag="VN444_03555"
     CDS             1499..1834
                     /locus_tag="VN444_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20920.1"
                     /translation="MKTLTIVAGVMLVFVFVWERVDVVRVGYHIERLKGQKVLLERER
                     DQLQVKFSALTAPERIAKVATDKFGLIPPMQGQVLMVHEPGEVPSAPLLPAEQVRVAR
                     HISSGRAGE"
     gene            1831..>2079
                     /locus_tag="VN444_03560"
     CDS             1831..>2079
                     /locus_tag="VN444_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HWR20921.1"
                     /translation="MTSGPSRGRRYVMLLLLLCGFGIILFRLVTLQVLQAAELTAKAD
                     RQHQKTVSFEGARGMVSDRHGKVLAMNVEVPSVFGVPTL"
BASE COUNT          441 a          522 c          615 g          501 t
ORIGIN      
        1 ccaatagaga ggtgatgccg ctgacgccgc tttgactgtt ataccgttga acgtaaaggt
       61 gcccccccca atcacaacaa tcaggcagag cgttctcacg gttatgaatg atcgcatcaa
      121 atcattctca atccgactcg aattcttgtc aataaaactc tcaacttcgt aggcttgagg
      181 gctcttcagt ggccatgagc caatggctat gctcctgcca atttctcacc ggacatgtat
      241 tcacgtggcg attaagggaa atggtagggc tcactgtgtt gcccaagagg taatggagct
      301 tcactagcgt ggcctggcgg agcgaacacc acacggcgag agcttgattg ttaggcgtca
      361 ccatcctgcc tgatgcactc cggtgaccta agcagtatga gcgaaatccc gacataccga
      421 cgatttcccc acacccctcc agatggcgca cacgtttccc tcgtcaaaaa ccagagactc
      481 ttagacttca taagtccatg tatttcctat tgacaagtct tttttgcggt gtatactgcc
      541 tgcgctggtg ggagaaagtg ggtgaatctg gccgtaaatg atttatggag gctccgcgac
      601 atattccggt tatggcggat gaggtcctgt tttggctagt tcatgaaggt gcccgaatct
      661 atcttgattg taccgtggga tacagtgggc atgctgaaaa acttcttgaa gcgactggtc
      721 cagacagcag gcttataggg cttgatcgtg atgcagcagc catagcagcg agtcgagaca
      781 ggctcgcgcg atttggggac cgtgtgcgcc ttctctatgg tcacttcatg ggtctgaagc
      841 agcatcttgc tgccagtggt attgggcgag tcgatggaat cttgtttgat cttggggtct
      901 cgtccgcgca gctggaagag cccactcgcg ggtttagctt tcagggggat agcctgttgg
      961 acatgcgtat ggatcaatcg atgagcggga ccgcagctga cctggtcaac cgatggccgg
     1021 aggcacagtt ggccgacacg attttccaat tcggcgaaga acggttttct aggcggattg
     1081 ctcgtgccat tgttcatgcg cgtgaacgcc atccgctggc cacgacgaaa gagttggtct
     1141 ccgtgattga aggggcggtt ccggccaatt atcggcatgg ccgtatccac tgtgcgacga
     1201 gaacgttcca agcctttcgc attgcagtga atcaagagct tgactgtctg gagccggcct
     1261 tgcgtgatgc ggttgacgtg ttgtctcccg gcggccgcct ctgtgtgatt tcattccatt
     1321 cgctcgaaga tcggattgtc aaacacacgt tccgggcgct ctccgggaag aacgatcccg
     1381 cactgtgggt gctcaccaag aagcctcagg ttcccaccag agaagagtcg aaccggaatc
     1441 ctcgatcccg cagtgccaaa ttgcgggccg ctcaacggat atccagggag ggttagttat
     1501 gaagacattg accattgtgg ctggtgtgat gttggtcttc gtgtttgtgt gggaacgtgt
     1561 cgatgtcgtt cgggtgggtt atcacatcga acggttgaag ggccagaaag tattgctgga
     1621 gcgagagcgg gatcagttgc aagtgaaatt ttccgcgctg acggctccag agcgaattgc
     1681 gaaggtggcg acagacaagt tcgggctgat tccgccgatg cagggtcaag tactgatggt
     1741 ccatgaaccg ggtgaggtgc cgagcgcacc actcctacct gcggagcagg tccgtgtggc
     1801 gagacatatc tcaagcggga gggctggcga gtgacttccg gcccttctcg cggtcgccgc
     1861 tacgtcatgt tgctcctact cctctgtggg ttcgggatta ttttatttcg actggtcacg
     1921 ctccaagtgc ttcaagctgc agaactcacg gccaaggcag atcggcaaca tcaaaagacc
     1981 gtgtccttcg agggagccag gggcatggtg tcggatcggc acggcaaagt gctcgcgatg
     2041 aatgtggagg tgccatctgt gttcggcgta ccgacgttg
//
LOCUS       DATMXI010000191         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_266471, whole genome shotgun sequence.
ACCESSION   DATMXI010000191 DATMXI010000000
VERSION     DATMXI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266471"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VN444_03565"
     CDS             complement(<1..840)
                     /locus_tag="VN444_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HWR20922.1"
                     /translation="MAKLMAPGGTRTMAFSVLEAGADTVYVGVRGWSRRGSDTELTDA
                     EVRELCAAARAQGKHVRVVLNTMPSSEEVPLLLKKVDMYAGWGVTGFMISDIGSMVQV
                     RSHFPDITIHVSVGAGLSNALEVKFYHELGASVVILPYRMGIEDVRAIKRSLDVGLEV
                     FLFRTENLDGIVCPGKCTMSSYFSSNRWLDNEGKDYFYGSANRGGDCLRVCQVGWEAT
                     ADDREIDGKFGLKGNPLLWLQELSDYIQAGVDYFKVPGRDRSDDLVCDIVRFYRKVVD
                     DLQA"
     gene            complement(866..1786)
                     /locus_tag="VN444_03570"
     CDS             complement(866..1786)
                     /locus_tag="VN444_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HWR20923.1"
                     /translation="MFELSTHIQGPKQLDEIDLSAYDALYLGDYTCPLYPGNFSRHIE
                     TLTSGVERVKSMGKRCYLSLYAIPKDSDLVWIKELLQGARGLSLDGVEVHNMGLLRIV
                     REILGDIPIHLGVFGNLYTHETARVLQAYGVERVFPNAELSLEELIYIRDHSPVEVIV
                     PLHGKIPLAISGTCFVTDYTGQVPLHCEESCSQGHWLTHEEWELKSIGRANLSGKDLC
                     MLEYLDRLVQSNLGLFYIYTLGETAAYVETAGRVYREALQNVGSNGEVSVVRWLDQLR
                     SVSHSGLCNGFYFGVSGQEYLSPQTLSPIA"
BASE COUNT          685 a          962 c          778 g          661 t
ORIGIN      
        1 ggcctgcaga tcgtccacga ccttccggta gaagcggaca atatcgcaga ccaggtcatc
       61 agaccgatcc ctccccggca ccttgaagta gtccacacct gcctggatat agtcggacag
      121 ctcttgcagc cagagtagcg ggttaccttt caggccgaac tttccgtcga tctcccgatc
      181 atcggccgtc gcctcccacc cgacctgaca gacccgcaag caatcaccgc cccgattggc
      241 gctgccgtaa aagtaatcct tcccctcgtt atcaagccac cggttggagc tgaaatagct
      301 gctcatcgta cacttgcccg gacagacgat cccgtccaag ttctcggtcc tgaacaggaa
      361 gacctccaga ccgacgtcga gggatcgctt gatggcccgg acatcctcga tccccatccg
      421 gtatggcagg atgaccacac tggcccccag ctcgtggtaa aacttcacct cgagggcgtt
      481 gctcagcccg gcccccacgc tgacatggat cgtgatatca ggaaagtggg atcgtacctg
      541 aaccatggag ccgatatcgc tgatcatgaa cccggtaacg ccccacccgg catacatgtc
      601 caccttcttc aagaggagcg ggacctcttc cgaactgggc atcgtgttca gaacaaccct
      661 cacatgctta ccctgagcgc gcgccgcagc gcagagctcc cggacctccg catccgtcag
      721 ttcggtgtcg gatccccgtc gactccatcc cctcacgccg acatagaccg tatcggcccc
      781 cgcttccagg accgagaaag ccatcgtccg tgttccccca ggcgccatca gcttcgccat
      841 cgttcgttcc tcctcgcgcc cttcgtcacg caatcggaga cagcgtctgg gggctgagat
      901 attcctgccc ggagacgccg aaataaaatc cgttgcaaag gccgctatgc gacaccgatc
      961 tcaactgatc cagccaccgt acaacggaca cctcaccgtt tgatccgaca ttctggagcg
     1021 cctcgcggta aacgcgtcct gccgtttcaa cataggctgc cgtttcaccc agtgtataga
     1081 tgtagaacag gccgaggttg ctctggacca ggcgatccag gtactcgagc atgcacagat
     1141 ccttgccgct caggtttgcc ctgccgatgc tcttgagctc ccactcttca tgggtcagcc
     1201 aatgcccctg ggagcaactc tcttcgcaat gaagcggcac ctgaccggta tagtcggtga
     1261 caaagcaggt tccagaaatt gccagcggga ttttgccatg cagagggaca atcacctcaa
     1321 ccggcgagtg atcccggata tagatcagtt cttccaggct caattcggcg ttcggaaaga
     1381 cccgttccac cccatacgcc tggagcaccc gcgcggtttc gtgtgtatac aggttaccga
     1441 agaccccaag atggatcggg atatcgccaa ggatctcgcg tacgatcctc agcaggccca
     1501 tgttatggac ttcgacgccg tcgagggaca gcccgcgagc accctggagc agctccttaa
     1561 tccagaccag gtcactgtcc ttcgggatgg catacaggct cagatagcat cgcttcccca
     1621 tcgacttcac ccgctccacc ccagaggtga gggtctcgat atggcgggag aagttgccgg
     1681 gatagagggg acaggtatag tcgcccagat agagcgcatc gtacgcagac agatcgatct
     1741 cgtctagctg cttgggacct tggatgtgcg tggataattc aaacatctcc acctccgccg
     1801 ttcgtttgag aatgagtagc gagtacgctg tagcccaaca gggcttaaaa gaacaatgca
     1861 cgtgcagtgc caatcgctct cggacatgag tgatgtaact cattgattcg attggtggag
     1921 tctggtagga aaaaggacgt tgtcggacta actcaccgag gtaaaataga caaaggggca
     1981 aacaccccaa actgggtcaa ttgccccatc tcatacgtgt ttcactcgga agagacgcca
     2041 gggcttatct caacatatag ggcaggcgct ttggagcggc ttacaagggg gaggttctcg
     2101 gacttcatct ccaggctttg cgaccgccga ggccacctcg gccccacaca tccagtgttt
     2161 gtgcgatctg agaatctacc ttttccacgc ccgctatacg gtttgcgtta gacgaaaacc
     2221 caagtggtgt actgcctcca ggcttgccct ccgacagcca ggcgaaggct caggtcctga
     2281 ctcaccagac cgaagaaact tgacagagca taaagatcgg tctagtttta tgcatacagg
     2341 aggatccaca atgagaacaa cgctagagat cgacgacaag ctcctcaagc aggcgcaggt
     2401 gcttactaag gccaagacga agaggtgatc caccgttctc tgaaggccct tatccgccag
     2461 cagccgatcg agcgtctcct tggaaaactt ggccactttc ctctggactt aaccccgggg
     2521 aaactcgcca agcttcgcgc cgatggctga tggatgggtt ctgatcgata ccgctgtcgc
     2581 tttgagctac cggtgtcatc ttcttcaccg cgataagcac tttgacctca tggcgcgcca
     2641 tatcgggctt cctgtctgga caacatagcg cacagccctt ctcgatccag aactccactg
     2701 tctgttgatg tggactccaa ttggccgtca cccatgggcg gcttcagcgt gactatatag
     2761 agggacagac actatttatt gaaatccttt cttggtcttc cactctgcaa cacgtgtatt
     2821 actcgtccag tttcctgcgc acctcccaga cgaacccgtc tcttcgcagt ggccgttctg
     2881 tcgtcgtgtg cattcgaatc tctgtcgttc cgaaagaccg cctggccata attccctcgt
     2941 tgcctcacat gatacgggtg gtctgatgca acgattcgag caatccgtgg catgcacgat
     3001 cctgaccgcg agccccacga atcgtcacaa taatggtgtc tgtccctatt tcctccatag
     3061 cggctcttca gcctgcccac ttatca
//
LOCUS       DATMXI010000192         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_72369, whole genome shotgun sequence.
ACCESSION   DATMXI010000192 DATMXI010000000
VERSION     DATMXI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72369"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VN444_03575"
     CDS             complement(<1..1087)
                     /locus_tag="VN444_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR20924.1"
                     /translation="MVNTETLSTEDVALRRQIKWLIGLRLLVAFLFLGSAGILALREH
                     PPFALTPLFVFIACACLLTILYLLALNRTTRLRRLCGLQVWIDVVLVTALVHYTGGIE
                     SPFAFVYIFPVLAAAILLSRRSSLLLAGGSSILYGALINIQLYGLMERAQFLSTGSVA
                     HDPAYALLQLFVNSVTLFLVALLGSQLAGRLKEAGRELEERRIDLRNLRTLHQDIVEN
                     IPSGIVTLDLEGKIVSLNRDAQRIIGVTAEQIQDKSWRETPFGAIKGLETFFSASTST
                     FGGLSQELEITRQDGQSIPIGINLTPMKSSEGRLVGLVGIFQDLTERKKTEARLRQAD
                     RLATAGQLAAGLAHEVRNPLAAISGCIE"
     gene            complement(1294..2294)
                     /locus_tag="VN444_03580"
                     /pseudo
     CDS             complement(1294..2294)
                     /locus_tag="VN444_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745544.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
BASE COUNT          511 a          682 c          654 g          447 t
ORIGIN      
        1 gctcaataca gccgctgatc gcggctaagg gattcctgac ttcatgcgcc aatccggccg
       61 ccagttgccc agccgttgca agtcggtcgg cttgacgaag tctggcctcc gtctttttcc
      121 gttccgtcaa gtcctgaaag atgcctacga gacctacgag cctgccctcg gatgacttca
      181 tgggcgtgag gttgatcccg atcgggatag attggccatc ttgacgcgta atctcaagct
      241 cctgggagag tccgccaaag gtggacgtag aagcggagaa gaacgtctca agccccttga
      301 tcgctccaaa aggggtctct cgccagctct tatcttgtat ctgctcggcc gtgacgccga
      361 tgatcctctg cgcgtcccgg ttcaacgaga caatcttccc ctcgaggtcc agtgtcacga
      421 tcccgctcgg gatattctcg acgatgtcct ggtgaagggt tcgaaggttg cggaggtcga
      481 ttcgtcgttc ttccagctca cgacccgcct ctttgagccg cccggcaagc tggctgccca
      541 ggagagccac aagaaaaaga gtcacgctgt tgacgaagag ctggaggagc gcgtatgcgg
      601 gatcgtgcgc gacgcttccc gtggagagga attgcgcccg ctccatgagc ccatagagct
      661 ggatgttgat cagcgctcca tagaggatgc tgcttccgcc ggcgagcaac aggctcgacc
      721 gtcgggacag gaggatggcc gcggccagga ccggaaagat gtagacaaac gcaaagggac
      781 tctcgatccc gccggtatag tgcaccaggg cagtaaccag gacgacgtcg atccagacct
      841 gcaggccaca gagccgcctc agccgcgtgg tgcggttcag cgcgagaaga tagaggatgg
      901 tgagcaggca ggcgcaggcg atgaagacga agaggggggt cagtgcgaag gggggatgct
      961 cccggagggc gaggatgcct gctgagccca aaaacaggaa tgcgacgaga agccggaggc
     1021 cgatcagcca cttgatctgt cgtctcagcg caacatcttc cgtcgacagc gtttccgtat
     1081 tgaccatcag cggtcagctc tcagcgcgtc attccgtgct tgacacgcct gccccgtacg
     1141 tgatacgggg gaatccagtc gggccctctg gataccggct tccgccggta tgacgaactc
     1201 gcggcaagcc gcggggtatc gacccgcagc gaaaatgacg accttggaat caatgtgaag
     1261 aagtggttga tgcactacac tataagctgt cccttagcgc cctattaccg tcaccaactt
     1321 gaagatgggc agatacatcg ccacgatcat gccgcccacg accactccga ggaataccat
     1381 cagtatcggc tcaatcaggg cggtcagatt tgcgaccgcg tgatcgacct cggcatcgta
     1441 gaaatcggca atcttctcca acatcgagtc aagcgctcct gtctgctccc cgacgctgat
     1501 catctgcacc accatgggcg gaaagacccc ggacgtcttc aacgggtcgg cgatagtctt
     1561 cccttcggcg atgctcacac gggtcttcat aatcgcctct tccacaacct tgttcccggc
     1621 ggttttcgcc gtaatatcca atccgtcaag gatggggacc ccgctggcga ttaaagttcc
     1681 cagggttcgc gtaaactttg caactgccac cttccggagt agcgatccga acaccggaat
     1741 ccacagaagc agcgtatcag cagccctcct acccttctcc gtcttataac tgaaccgaat
     1801 gccaacgatc cccgcaatga ggaagccgag gccggccaaa agatacgtct gaatgagacg
     1861 actcaactcc atgacaattc gagttggcag gggaagctcg acccccgccc cctcgaacaa
     1921 cttcacgaag gtcggaatca cgaagaccag caaaaagatg acaaccgcga tggcaaccgt
     1981 cacgatcgtg ctaggataaa tcaaggcgcc tttgaccttc cgccgaaggg tcatcgcctt
     2041 ctcgatgtac accgcaagac ggctcaggat ggtgtcgagc aaccctccag tctcgcccgc
     2101 ctcgaccatg ttggcgtaca ggggattgaa tacggtcgga tggcgtttga gagctgccgc
     2161 aaaggtcgaa cccgcctcca cgtcccctca gatctcagtg agggttttct tgaactgttt
     2221 attcggctgt tgggatgcca aggtatccag gcattgcacc agggggagtc cggcgtcgat
     2281 catggtagca agct
//
LOCUS       DATMXI010000193         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_276535, whole genome shotgun sequence.
ACCESSION   DATMXI010000193 DATMXI010000000
VERSION     DATMXI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276535"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VN444_03585"
     CDS             <1..1343
                     /locus_tag="VN444_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20925.1"
                     /translation="HMPAKSVVIGGLRKRSDLGFRGLTVGELTQMAGRAGRRGIDPEG
                     TCLLALDSAEAAEDAVRLVRGDPEPIESQFRIGYSSAALLIALYREPEAIRKTIEKSF
                     GQFQNRRKIETIRREQAGVIRRLADAEGIASPCCSVSTLIEYRERRAALEQERERLMA
                     LRVATARAGRTGGRGRRRGRARPGPSSLSFFTGESADEGRAKLDAMALGLSQMPCHRC
                     PERGRRERQIKQSRQLGQMLERHHQTQQQLQDSYWEQFLRVVAILQHFGYIENGRLGA
                     EGLLIASLRHDNELLVARVAFSGLLDGLSPEELAALLSCLIEEPRATEQVAAKLFLRD
                     QAHLRRRVKALEGLSQEVDRVQRSYQVNLPVSMHTTYLAATHRWAAGEDDWIALVEQS
                     FGGHEGDLIRAFRRLIDLCRQLEESPELPADLTRTLSRATAMLDRGIVLESALI"
     gene            1545..3017
                     /locus_tag="VN444_03590"
     CDS             1545..3017
                     /locus_tag="VN444_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWR20926.1"
                     /translation="MVARRVTTPQAIGIGGSPLASPYEGVALLADPIHRYIVFTVPKG
                     DRSERTEKEIIDSPWVQRLRRIHQLQSSWWIYPSGEHSRFQHVLGTMHMAGAFARHLY
                     PSLKETFGEALPSEPFIEELLRLAGLLHDVGHGPFGHFFDDHFLQQFHVGGERLNHEI
                     LGMAIITKRLGGIIKAIRRSPSGPFPSNERLDPRQIAFLIKKPEAGPVADFPDWLLAL
                     RPLFSGIYTVDNLDYVQRDAYMTGFSLDMVDIERLLLYTFFTPKGLTLHKAGESALMR
                     FLNAKIALYVNVYHHRTTRAIDLHIQEIFKGTMARIAPYNPYKTLDRYLELTDWFLLE
                     RVREWATSKDPIERSLGAEWGKILGRKVKWKMAYDFVSTIEESQRMVQFQTATHLEEA
                     IRRHLSKRLCGIEFKVDMAALDTRPLNPLAEGSKQIFMYNPATGEVSPRPLMDLFRDI
                     PPKVARYRVFTTDRRHVKALSEASEKALTGSAGESVPTNI"
     gene            3197..>3440
                     /locus_tag="VN444_03595"
     CDS             3197..>3440
                     /locus_tag="VN444_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR20927.1"
                     /translation="MTICTRNRECLFGDVADGAMHLNDAGRLVQTVWDELPARCPGVC
                     LDAFVVMPNHVHGIITVGAPLVGAQEGIPDDRATTRV"
BASE COUNT          682 a         1001 c         1093 g          664 t
ORIGIN      
        1 ttcacatgcc ggccaaaagc gtggtgatcg gaggacttcg taagcggagc gatctcgggt
       61 tccgtggact caccgtgggg gagctgactc agatggccgg gcgagccggg agacggggga
      121 tcgatcctga agggacctgc ctcctggcgc tcgattctgc tgaggcggca gaggacgccg
      181 ttcgtctagt gcgaggcgat ccggagccga tcgagagcca attccggatc ggctacagca
      241 gcgccgcctt gctgatcgct ctgtaccggg agccggaggc gatccgcaag accatcgaga
      301 aaagctttgg ccagttccag aatcgtcgga agatcgagac catccggagg gaacaggcgg
      361 gagtgatccg caggctagcc gacgccgagg gcatagcatc accctgttgc tcggtcagta
      421 cgctgatcga gtatcgggag cggcgcgccg cgctcgagca agagcgggaa cggcttatgg
      481 cgttgcgggt ggcgaccgcg cgagccggcc gtacaggggg tcgtggccgt cgtcgtggtc
      541 gtgcccgacc tggtccctca tcgctcagtt tcttcaccgg agaatcggcg gatgagggca
      601 gggcgaaact cgacgcgatg gctcttggcc tttcccagat gccctgtcac cgctgccccg
      661 aacgggggcg ccgagagcga cagatcaagc agtcgcgaca actgggacag atgctggagc
      721 gtcaccacca gacgcagcag cagctccagg attcctactg ggaacagttc ctgcgggttg
      781 tggcgatcct gcagcacttt ggttacatcg agaatgggcg gttgggcgcg gaaggtctcc
      841 tcattgcatc actgcgccac gataacgaac tgttggtggc ccgggtcgct ttctccggtc
      901 ttctggatgg gctttcaccc gaggagcttg cagcgctgct ttcgtgcctg atagaggaac
      961 cgcgagcaac cgagcaggtg gccgccaagc tgttcctccg cgatcaggcg caccttcgcc
     1021 ggcgcgtgaa ggcgctggaa gggctgagcc aggaggtgga cagggttcag cgatcctacc
     1081 aggtcaacct tccagtctcg atgcacacga cctatctggc ggccacccat cggtgggccg
     1141 caggcgagga cgactggatt gcgctggtcg agcagagttt cggagggcac gagggtgacc
     1201 ttattcgggc attccgccga ctgatcgacc tgtgccggca gctcgaagag agcccggagc
     1261 tgccggcgga tctgacgcga acgctgtcgc gggcaactgc gatgttggat cgtggcattg
     1321 tcctggaatc cgcattaatc taagagggtg ataactactc aggtgtttag gctgtaggct
     1381 gaaggctgtt agtcgttgcg catggaccga atcaaggcgt ccagaacatt ttcggtctcc
     1441 atcattattc gcgtcgcatt cggaaacgcc tggcttatct gtgcgcgttc ctacagtcta
     1501 aaagactaca gtctatctag ctaggtagtt acaaggagca atcgatggtt gcgcgacgcg
     1561 tcaccacacc gcaagcgatt gggatcgggg ggtcgccact cgcgtcgccc tacgaagggg
     1621 tggcgttact ggcggacccg atccaccgct atattgtctt caccgtgccg aagggtgacc
     1681 ggtcagaacg gaccgagaag gagatcattg atagcccgtg ggtccaacga ttgcgccgca
     1741 tccaccaact acagagcagt tggtggatct acccatccgg cgaacacagt cggtttcagc
     1801 atgtcctggg gaccatgcac atggccggcg cgttcgcccg acacctctat cccagtctga
     1861 aggagacatt cggggaggcc ctgccgtcgg agccgttcat cgaagaactc ctgagacttg
     1921 ccgggttgct ccacgacgta ggacacggcc ccttcgggca ttttttcgac gaccactttc
     1981 tacagcagtt tcacgtcggc ggcgagcggc tgaaccacga gatcctgggg atggctatta
     2041 ttacgaagag gctagggggg atcatcaaag cgatccgccg aagcccgagc gggccgtttc
     2101 cgtcgaatga gcggctcgac cccaggcaga tcgcctttct gatcaagaag ccggaggccg
     2161 gccccgtggc tgactttcca gattggctgc tggcgctacg cccgctcttc tccgggatct
     2221 acaccgtgga caaccttgat tacgtccagc gagatgccta tatgaccggc ttctcgcttg
     2281 atatggtgga catcgagcgg ctgctgctgt acacgttttt cacgccgaag gggctgaccc
     2341 tgcacaaagc cggagagtcg gccctgatgc ggttcctgaa cgcgaagatc gccctttacg
     2401 tgaacgtcta ccatcaccgg acgacccggg ccatcgacct gcacatacag gagatcttca
     2461 aggggacgat ggcgcggatc gccccgtata atccgtacaa aacgctagat cgatatctgg
     2521 agctgaccga ttggttcctg ctcgagcggg tccgcgagtg ggccacctcg aaggatccga
     2581 tcgagcggtc gctgggagcg gaatggggga agatcttggg ccggaaggtc aagtggaaga
     2641 tggcctatga cttcgtgtcc accatcgaag agtcccagcg gatggtccag tttcagaccg
     2701 ccacacatct ggaggaggcg atccgccgcc atctatccaa gcgtctgtgt gggatcgagt
     2761 tcaaggtgga catggccgcg ctcgataccc gtcccctcaa cccgctggcg gaagggagca
     2821 agcagatctt tatgtacaat ccggccactg gcgaggtgtc gcccagaccc cttatggatc
     2881 tatttcgcga tatcccaccc aaggtggccc gctaccgggt cttcaccaca gaccggcggc
     2941 atgtcaaagc cctcagcgag gcctccgaga aggcgctgac cggttccgcc ggcgagtcgg
     3001 ttccgacgaa tatctgatcc agatgccctc gctgagccat tcgccggggt atgtcactgt
     3061 aggggcgctg cttgctgcgc ccctcataat cagagcaagc agcggcccca cgggcgcagc
     3121 aagcagcgcc cctacgaacc gccgtcagtc ccttcgcctg aaagaattcg attattccga
     3181 ggagggcgcc tatttcgtga cgatctgcac acggaaccga gagtgcctct ttggtgatgt
     3241 tgcggatggg gcgatgcacc taaacgacgc aggtcggctg gttcagacag tatgggacga
     3301 attgcctgct cggtgtcccg gcgtatgcct ggatgcattt gtggtgatgc cgaaccacgt
     3361 ccatggaatc atcaccgtag gggcacccct cgtgggtgcc caagagggaa tccctgacga
     3421 tagggcaacc acaagggttg
//
LOCUS       DATMXI010000194         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_158990, whole genome shotgun sequence.
ACCESSION   DATMXI010000194 DATMXI010000000
VERSION     DATMXI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158990"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..376)
                     /locus_tag="VN444_03600"
     CDS             complement(71..376)
                     /locus_tag="VN444_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20928.1"
                     /translation="MKTWSSLICASVLFLAGCAEGAKLVQDTGDGGLVTYPFKGEQGA
                     MLSSFRSEALALMRDKCSGSYTIVREGETRGRARADSVEGSQEMIHERRWGIQFQCK"
     gene            451..876
                     /locus_tag="VN444_03605"
     CDS             451..876
                     /locus_tag="VN444_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR20929.1"
                     /translation="MNTRIAMFVAGLVVFLAATVTVVSAHHSYLLTVQQLKVGLTKAP
                     SMAEKGFVLIDVRSPEEHLQGFIPGTDINIDFREMRTRHREIGAQLEDHIVVYCQSGH
                     RSNIAAETLADLGYRHVYNVSGSMNAWLAAGFPVESTRR"
     gene            complement(896..1786)
                     /locus_tag="VN444_03610"
     CDS             complement(896..1786)
                     /locus_tag="VN444_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20930.1"
                     /translation="MAQNTHFQLSLPVARVVLSITLFMFSAVSTTPAQTITEDQLGDG
                     LSVSVWNPQTQCPDVAPLVAVEIDPDRYRFSVHYYQQDGFSSPLDIRQWQERTGHDLV
                     FNAGLFRENYAYLGLLYGNGRSLGSKRHPTWLGLFVAEPSVDRTRRARVLDLTFDTFD
                     EGHPPYREVAQSLMLLDRTGTIRVRQTGKRAHQTLVAEKGDGHLLLFKTTEVTSLHAL
                     GECLRDAFPPLRQVMAMDGGSSSDMAVSSALRQARPKMAGLHSWLSLLDGSNLTHIGL
                     PAVIGVSPRQEQGGAKTGAR"
     gene            complement(1877..3502)
                     /locus_tag="VN444_03615"
     CDS             complement(1877..3502)
                     /locus_tag="VN444_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20931.1"
                     /translation="MKPPLRLLHLEDDSVDAELVATTLVEGGIPCQSQVVDTRQAFVA
                     ALKECRMDLILADYSIPGFDGMTALTLARQHCPDVPFLFVSATIGEELAIDAMHHGAT
                     DYVLKQRLGRLVPSVQRALRELDDRAERKRAEEALRQSEKQFRQSQKMEAVGRLAGGI
                     AHDFNNLLTVIMGYSQVLLTELGPQHPLRGKIDETLKAGERAATLIRQLLTFSSKQSL
                     DPKILSLNTAVTSLESLLRRLIGEHIQLVCRLDPTHARLKADQSQLEQVLANLVVNAR
                     DAMPSGGTLTIETAQVELTRSPVYHLTPLLPGQYVRLTVSDTGCGMDRKTEAHIFEPF
                     FTTKAEGKGTGLGLSTVFGIVTQCGGAIDVTSRVGHGTRFDLYFPSVESDIPATVTPG
                     PLAQPQRGTETILLVEDEPSVRTLVRDELRKLGYRVVEAKNGVDACLLATQQAGSFQL
                     LLTDVVMPGMGGRELAQHLSVIKPDLRTLFISGYMDDIGIMAGQEEGISAFLQKPFTP
                     EVLAHAVRNLLDSSAVSETAGASIPSTIPKMPR"
     gene            complement(3531..3974)
                     /locus_tag="VN444_03620"
     CDS             complement(3531..3974)
                     /locus_tag="VN444_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR20932.1"
                     /translation="MTTVKPILLAEDNPRDAELALAAMEEHHIADKVVVCRDGAEVLD
                     YLYCRGIFSTRQEGNPVVVFLDLKMPKINGLEVLRTIKSDVNLKPIPVVMLTSSREES
                     DLAQSYALGANAYVVKPVEFHQFITAVKELGVFWGVINEPPPEGS"
BASE COUNT          868 a         1134 c         1152 g          899 t
ORIGIN      
        1 gtacaggaag gggtctcgct gatcagtctg ctgacgatcg acgatctgac caaagatctc
       61 tccacatgag ttatttgcac tgaaactgaa taccccaccg gcgctcatgg atcatctctt
      121 gcgagccctc aacgctgtcg gcacgtgccc ttccccttgt ctctccttcc cgcacaatcg
      181 tgtaggaccc gctgcactta tcacgcatca acgcgagtgc ttcgcttcga aatgaagaga
      241 gcatggcccc ttgttccccc ttgaacgggt aggtcaccag ccctccgtca cctgtgtcct
      301 gcacgagttt tgccccctcg gcgcagccag ccaggaagag aacggatgca cagatgaggc
      361 ttgaccaagt cttcatggag tccctatcat aatcatgaag gtcgttctct gtcgagtatg
      421 tccatcatgg ctgtcatcgg gaggcctatc atgaatacac gaatcgccat gttcgtcgcc
      481 ggtcttgttg tttttctcgc cgcgaccgtg accgtggtgt cagcccatca ctcgtatctc
      541 ttgaccgtcc agcaactgaa agtcggactg acgaaagccc cttcgatggc cgaaaagggg
      601 tttgtgttga tcgatgttcg atccccagaa gaacatctgc aaggcttcat ccctgggact
      661 gacatcaaca tcgattttcg tgaaatgagg acacgccacc gtgagatcgg cgcgcagttg
      721 gaggaccaca ttgtggtcta ttgtcaatca ggccatcgca gcaacattgc agcagaaaca
      781 ctggccgatc tgggctatcg ccatgtctac aacgtcagcg gaagcatgaa tgcctggctc
      841 gcggctggct tcccggttga atcaacgcgt cgttagctca cagatgtgac cgaactcatc
      901 tggcaccagt tttcgcacca ccttgttcct ggcgtgggct gaccccgatc acagccggaa
      961 gtcctatgtg ggtcagattg cttccgtcca acagtgaaag ccaactatgc aatccggcca
     1021 tcttaggcct ggcttgtcgc agtgcggagc tgacagccat gtccgacgag gatcccccat
     1081 ccatggccat gacctgacgc agggggggaa aggcgtcccg caggcattcc ccaagggcat
     1141 ggagcgacgt cacttcggtc gtcttgaata agagaagatg cccatcgcct ttttcggcaa
     1201 ccagagtctg atgcgcgcgt ttgcctgttt gacgcacgcg gattgttccc gtccggtcaa
     1261 ggagcatgag ggattgtgca acctcgcgat agggagggtg cccctcatcg aaggtatcga
     1321 aggtcaagtc caagacccgc gctcgccttg tacgatccac cgatggctcc gccacaaata
     1381 atcccagcca cgtggggtgc cgcttgcttc ccagcgaacg gccatttcca tagagcagac
     1441 cgagatacgc gtaattctca cgaaagagcc ccgcattgaa caccagatcg tggccggttc
     1501 gttcctgcca ttggcgaata tcgagcgggc ttgagaaacc gtcttgttgg taatagtgca
     1561 cggaaaagcg atagcgatcg gggtcgatct cgacggcgac caatggcgca acgtccgggc
     1621 attgtgtctg aggattccag acagaaacgg ataaaccgtc tccgagttga tcctctgtga
     1681 tggtctgagc cggcgtcgtc gagacagcac tgaacatgaa aagagtgata gacagcacaa
     1741 cccgagcgac agggagagac agttggaagt gagtgttctg tgccatggtg tctcacgatc
     1801 gagtatagag agcgctggta cagagcgtca aatcattcga ggaagagggt cctcagctgc
     1861 gatgttgctc ttgcggtcag cgcggcattt ttggaattgt cgatgggatg gaagcgccgg
     1921 ccgtctcgga cactgcactg gagtcaagca ggttacggac cgcatgggcg agtacctcag
     1981 gagtaaaggg tttctgcaag aaagcgctta taccctcttc ttgccctgcc ataatgccga
     2041 tgtcatccat atagcctgaa atgaacaggg ttcgtaggtc cggtttgatg acggagagat
     2101 gttgtgccag ttcccgtcca cccatgcccg gcatgacgac gtcggtcagg agaagctgaa
     2161 acgatcctgc ttgttgcgtc gccagcaagc aggcatcgac tccgttcttg gcttctacga
     2221 ctcggtaacc cagtttccgc agctcgtctc gcacgagcgt gcggacgctt ggttcatctt
     2281 ccaccaagag aatcgtttcc gtgcctctct gaggctgggc caagggtccg ggcgtcacgg
     2341 tggcagggat gtcagactcg acactgggga agtacagatc aaaacgcgta ccgtgaccca
     2401 ctcggctggt aacatcgatg gcaccgccgc attgagtaac gatcccgaac accgttgaga
     2461 ggcctaaacc cgtacccttc ccctccgcct tcgtggtgaa gaacggctcg aagatatggg
     2521 cttctgtctt acggtccatg ccacagccgg tatcgctgac agtcaaccgt acatactgac
     2581 ctggcagtaa cggggtgagg tgatagacag gactgcgggt taattccact tgggcggttt
     2641 cgatcgtgag cgttccgcca ctcggcatgg cgtcccgcgc attgacgacg aggttggcca
     2701 gaacctgttc aagctgagat tggtcggcct tgagtcgcgc gtgtgtcgga tcgagtctac
     2761 agacgagctg gatgtgctcg ccaataagac ggcgcagcag actttccagg cttgtgacag
     2821 ccgtattgag ggacagaatt tttgggtcca gcgactgttt gctgctgaac gtgagtagtt
     2881 gtcggatcag cgtcgctgcc cgttcaccgg ctttcagtgt ttcgtctatc tttccgcgaa
     2941 gaggatgttg agggcccagt tcggtcaaga gcacttgact gtagcccatg atgacggtca
     3001 ggagattatt gaaatcgtgc gcgataccac cggccagcct cccaaccgct tccatttttt
     3061 gggactgccg aaattgtttc tcgctctgcc gaagggcctc ttctgcccgt ttcctttcgg
     3121 cgcggtcatc gagctcacgc agtgcacgct ggaccgaggg cacaagccga ccgagccgtt
     3181 gtttgagcac atagtccgtc gcgccatgat gcatggcgtc gatggccagc tcctctccga
     3241 tggtggccga gacgaaaaga aagggaacat cgggacagtg ctggcgggcc aaggtcaggg
     3301 ccgtcatgcc gtcaaagccc gggatcgaat agtccgccag gatgagatcc atccggcatt
     3361 ccttgagagc cgccacaaat gcctgtctcg tgtcgacgac ttgcgactgg cagggaatcc
     3421 cgccctcgac caaggtggtt gcaacgagtt ctgcatcaac cgaatcgtct tcgaggtgaa
     3481 gtagacggag tgggggtttc acaggtcctt cgtttggatg ttacttgagg ctaagagcct
     3541 tcggggggcg gttcgttgat tacgccccaa aagacaccaa gctcttttac ggccgtgatg
     3601 aattggtgaa actcaaccgg tttcaccacg taggcgttgg cacccaaggc gtagctttgg
     3661 gccaaatcgc tctcctcgcg cgaggatgtg agcatgacga cgggaatcgg tttcagattg
     3721 acgtcactct tgatcgtcct cagaacttcg agcccattaa tcttcggcat cttgaggtcg
     3781 aggaacacga ccacgggatt cccctcctgc ctggtggaaa agatgccacg gcaatagagg
     3841 tagtcgagca cttccgcccc gtcacgacat actacgacct tgtcggcaat gtgatgttct
     3901 tccatggcgg ccagcgcaag ctcggcatca cgagggttat cctccgcgag aagaatgggt
     3961 ttcacagtgg tcatctgaga gacctcgcga gtggaagtga aaagtagaag gtggcccctt
     4021 ggtccagcgc tccttcagcc cacgtccttc ccc
//
LOCUS       DATMXI010000195         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_44576, whole genome shotgun sequence.
ACCESSION   DATMXI010000195 DATMXI010000000
VERSION     DATMXI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44576"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VN444_03625"
     CDS             complement(<1..919)
                     /locus_tag="VN444_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWR20933.1"
                     /translation="MTTLLLGVGLLVFFYRLDSLSLFDADEPAYAEAAREMLISGDWV
                     TPHFNFQPRFDKPILFYWLIALAYKGFGIGEYAARFWSAAFATGLTLSIYLFGRQLLG
                     RRGACIAALAFATNVGTIVLGRAAVTDMTLAFCMAWTLFCFFDVYRATDKARESLLFV
                     GYLAMALAVLTKGPIGLLLPGLVIGLFLIIRRKAQATLSRLRLLSGLALFTVIALPWY
                     VLVLRENGWAFVQGFFVQHHLNRYLGVVSSHVGGPLYFLPVVALGFFPWSGTLPNAFG
                     HLWAIRSRLRTELTERQELLLFLWVWCGVV"
     gene            complement(972..1637)
                     /locus_tag="VN444_03630"
     CDS             complement(972..1637)
                     /locus_tag="VN444_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWR20934.1"
                     /translation="MRSRLQARSKPSGSLARSFDGKVLGYTAALFAVLPFLVLYVDGS
                     VKTFILSVQSDWGLIMAGAITALGYGPVAAAVAATLLVIGLKAGKHREAIAGKFGLLA
                     VILGGLSGQVVKNLACRSRPLAEKSGQFFVEFPCLGKGAGFISFPSGHSVTAFAIAFV
                     LSRTYPRYAFLFYGLAGLVALSRVYLAKHFPSDVVAGAAIGLLAGWIVCRFSAFSSVH
                     GHT"
     gene            complement(1651..1911)
                     /locus_tag="VN444_03635"
     CDS             complement(1651..1911)
                     /locus_tag="VN444_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWR20935.1"
                     /translation="MMMRWEEMTPEPWFWGWGILWMVMITAFWLLILLVLVLTVRWLW
                     RAGSGIRYVQLPEESAQEILKKRYAKGEIGKEEFEAKKRDLL"
     gene            complement(1953..2954)
                     /locus_tag="VN444_03640"
     CDS             complement(1953..2954)
                     /locus_tag="VN444_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HWR20936.1"
                     /translation="MIRLRIAVLFLAAVVITGIAGYRFLEGYTWLEAFYMTIITISTV
                     GFQEVRPLSPAGRLFTIGLLFAGLGVVLYTAGTVTAKIVEGEFQQFFGRRRMEKQIDA
                     LTNHYLVCGYGRIGEVICRELASKPVPFVVIELEEERVRKVEEAGYLLLKGDATDEKV
                     LLVAGVIRAKGLFATMPIDADNVFMVLTAKDLNPSIFVVARAETERSEKTLAHAGANK
                     VISPYAMGGHRMAQAALRPAVVDIIELATHYRSLELQLEEIVVPVGSPCEGVTLGDSG
                     LCKEPGVIVVAIKRALGEMIFHPSTNERIEAGDRLVAMGEIARLRWLERQVESTSST"
     gene            complement(3022..3609)
                     /locus_tag="VN444_03645"
     CDS             complement(3022..3609)
                     /locus_tag="VN444_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWR20937.1"
                     /translation="MAAKKILMLVGDFVEDYEAMVPFQMLTMVGHTVHAVCPGKRAGD
                     TVRTAVHDFEGDQTYSEKRGHNFALNATFAKIRPKNYDALVIPGGRSPEYLRLNEQVL
                     DIVRHFAKENKPIASICHGQQLLTAAGVVEGKRCTSYPAVRPELIRAGAKWEEVNATF
                     SNAYVDGNLVTAAAWPGHPEWMRKFLDVLGSKIEP"
BASE COUNT         1022 a         1263 c         1118 g          781 t
ORIGIN      
        1 cgacgacacc acaccagacc cataaaaaga gcaacaactc ctgccgttcg gtcagttcag
       61 tacgtaatcg acttcggata gcccagagat gaccaaaggc attgggtagc gtcccgctcc
      121 acggaaaaaa accgagggcg accaccggca agaaatacag gggtcccccg acatggcttg
      181 acaccacacc taggtagcgg ttcaggtgat gttgtacaaa gaatccttga acgaacgccc
      241 atccgttctc gcgcaggacc aagacgtacc aggggagggc tatcacggta aatagcgcaa
      301 gtccgctgag taaccgaagc ctggaaagag tcgcttgggc ctttctgcga atgatcaaaa
      361 ataggccgat aaccagcccg gggagcaaaa gaccgatcgg gcccttcgtg agaacggcga
      421 gcgccatagc cagatatcca acaaagagga gactctcgcg cgccttatct gtggctcggt
      481 agacatcgaa aaagcagaag agtgtccagg ccatacaaaa ggcgagcgtc atgtcggtaa
      541 ccgccgcccg ccccaggacg atggtcccga cgttcgtggc aaaggccaga gcggcaatac
      601 atgcgccccg ccggccaagc agttgccgtc cgaacaggta gatcgacagc gtgagtccgg
      661 tcgcgaaggc ggcagaccag aagcgagccg cgtactcgcc gatcccgaaa cctttgtagg
      721 ccagcgcaat cagccagtag aataagatcg gtttatcgaa gcggggctgg aagttaaagt
      781 ggggtgtgac ccagtctcca gagatcagca tctccctcgc cgcctcagca taggccggct
      841 catctgcgtc aaacagcgag agcgaatcca ggcgatagaa gaagacgaga agacccacac
      901 caaggagcag ggtcgtcacg gcaacttcta gaacccatcg gcgagagcta ccgacagcag
      961 gtgtcgcctt ctcaggtgtg cccatgcaca gaggaaaagg ccgaaaaccg acagacgatc
     1021 caacctgcca gaagcccgat cgcggctccg gccacaacgt cagaggggaa atgcttggcg
     1081 agatagaccc tggagagggc gaccaggccg gccaatccgt agaaaaggaa cgcatacctg
     1141 ggataggtgc gggagagtac aaatgctatg gcgaaggcag tcacagaatg gccggatgga
     1201 aaggaaatga agccagcgcc cttacccagg caagggaact cgacgaaaaa ctggcccgac
     1261 ttttcagcga gggggcggga tcggcaagcc agatttttca cgacctgacc ggagagacca
     1321 ccgagaatca ccgcaagtaa tccgaatttg cccgcgatgg cctctcgatg ctttccagcc
     1381 ttcaggccga tgaccaaaag ggtagcagcc acggcggcag ccacggggcc atacccaagg
     1441 gcggtgatcg cacctgccat tatcaacccc caatcgctct gcaccgatag gatgaaggtc
     1501 ttcaccgaac cgtctacata cagcacaaga aagggtagta cggcgaataa cgctgcggta
     1561 taacccaaca ccttgccgtc gaacgaccgg gcgagggacc cggaaggctt cgagcgagct
     1621 tgtaatcttg accgcatcct gtctatcccc tcacagcaaa tcgcgcttct tggcctcgaa
     1681 ctcttctttc ccgatctcac ctttggcata ccgcttcttg aggatctcct gggccgattc
     1741 ctccggcaac tggacgtatc taatcccgga accagcccgc caaagccagc gaaccgttag
     1801 caccagcacc agcaaaataa gaagccagaa ggccgtaatc atgaccatcc aaaggatccc
     1861 ccatccccag aaccagggct caggcgtcat ctcctcccac cgcatcatca tgacccttac
     1921 tccgcgctcg cacaagggtg ggaacgcatc ttttatgttg aagaagtcga ttccacctgc
     1981 cgctccaacc acctcaggcg agcaatctct cccatggcga caagacgatc cccggcctca
     2041 atcctttcat ttgtggaggg atgaaagatc atttcaccca atgcccgttt gattgccacc
     2101 acaatcaccc caggctcctt gcacagccct gagtcgccca gcgtgacccc ctcgcaggga
     2161 gatcccaccg gcaccacaat ctcctcaagc tgcaactcca ggctccgata gtgcgtggcc
     2221 agctcaatga tatccactac ggcggggcgg agggctgcct gagccatgcg gtgtcccccc
     2281 attgcgtagg gcgaaataac cttattggct cctgcgtggg caagggtctt ttcgctccgc
     2341 tccgtctcgg ccctggcgac tacaaagata gaggggttga gatccttggc cgtgaggacc
     2401 atgaagacat tgtccgcatc gatcggcata gtggcgaaga gacctttggc ccgtatcacc
     2461 ccggcaacca ggaggacctt ctcatcggtg gcatccccct tgagcaaaag atagcccgcc
     2521 tcctccacct tgcggacccg ctcttcctcc agctcgatga ccacaaacgg gacgggcttt
     2581 gaagccaact cgcgacagat cacctccccg atgcgaccat agccacacac gagatagtgg
     2641 tttgtcaggg cgtcgatttg cttctccatt ctcctcctcc cgaagaactg ctggaactcc
     2701 ccctccacga tttttgcggt aacagttcct gctgtataga ggaccacccc caggccggca
     2761 aagaggagcc cgatcgtgaa gagcctgccg gccgggctca agggtcttac ctcttgaaac
     2821 cccaccgttg agatggtgat gatggtcata tagaaggcct caagccacgt atacccctca
     2881 aggaagcgat accctgcaat ccctgtgatc actactgccg cgagaaacag tacagcgatc
     2941 cttagtcgta tcaagggagt gactcctctg tcacccggtc agtcctcacc gtcgagagtt
     3001 gagacgaatt catgatgata tttagggctc gatcttggaa cctaacacgt ccaggaactt
     3061 gcgcatccat tcaggatggc ctggccaggc tgcggctgtg acgagattac catctacata
     3121 cgcattggag aaggtagcgt tcacctcttc ccacttggcg ccggctcgga tcagctcagg
     3181 cctcacggcc ggataggagg tgcagcgttt gccttccacg acaccggccg ccgtcaagag
     3241 ttgctgtcca tgacaaatgg aggcgatagg tttgttctcc ttcgcaaagt gtcggacgat
     3301 gtcgagcacc tgctcgttca ggcgaagata ttccggtgac cgtcctcccg gaatcaccag
     3361 ggcgtcgtaa ttcttgggcc tgatcttagc gaaggtggcg ttgagggcga agttgtgacc
     3421 ccgcttctcg ctgtacgtct ggtccccctc gaagtcgtgg acggctgtcc gtaccgtatc
     3481 gccggctcgc ttgccggggc agaccgcgtg aaccgtatgc ccgaccatgg tcaacatctg
     3541 aaaggggacc atcgcctcgt agtcctcgac gaaatccccc acaagcatca gaatcttttt
     3601 cgcagccatc atcttcctcc ctgtacacac gagatataca cgagggcata ttaactgtat
     3661 caaaattgaa ttggcatcga ttacgaaaat ctccccctac ccctcttttc caaagagggg
     3721 tcaatcctcc ctttggcaaa gggaggtcag gagggattct tcgagaacgt acagtgatta
     3781 ttctgagaca gttaatacgt ctcagccagt ttgtcattcc gggcttgacc cggaatccag
     3841 cacccttctg gattcccgct tgcgcgggaa tgacgtccat tgttgccagg gatgacttat
     3901 gtatagcagg cccagaatcc atcccagggc gaggctatca aagcgaccct cactccgcaa
     3961 gcagatttca caagcgccac caggcagggc aagcagatga tatcactccg gtgaggatgg
     4021 agatcatggg aaaagatcag atagaatgaa gtggggaagg gctagaccag ccagtcaccg
     4081 ggatgggcct actcttctcg aaactccggc gcgccgggtt tcccgggctc tggcacctca
     4141 tgggtgcagc gtaaacgtct cagaacccaa ccgctagggt gcga
//
LOCUS       DATMXI010000196         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_285911, whole genome shotgun sequence.
ACCESSION   DATMXI010000196 DATMXI010000000
VERSION     DATMXI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285911"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..2222
                     /locus_tag="VN444_03650"
     CDS             60..2222
                     /locus_tag="VN444_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20938.1"
                     /translation="MASTKRPAREGPASYEDHDGIRAYLEAVEESDRQAREVREREAL
                     YHLLLYIGLQWVTWDRLLGTYRPEGLKPTTPQPVTNKFAVGINALVASTSAFNPPLAF
                     HPATEDADDIAAANVADHVLEVVKHEVKHRDLLQIEAKWLFLTGNVFRVTHYQPDLLG
                     PMSFVQHERCQMCQQVSPPAVLLEQNNTCPHCGEQGPFVPAEDEVGQPIGDTVPRGVM
                     TCEAHSVLTVRAASEADSLEESPYVVIDQQKTMEWVERTYGEEIAAKVDHETSESPSR
                     WIMQAAIHSTGAGLMTGSGQQDQPSPQVRVRRLWMRPTPTFPGGLYAEVVGNQVVHSA
                     EWPYHDREGRPILNITHTRCDVVPGRLWGKTRASDVAQKQVQRNKLEAVMTLFERRSS
                     NDILMVPSGSGITKVTGQPGLMLQYNAMAGVPPPHREPGHPPPPYFIQRIQQIDEEID
                     ALFGTYEILRGDAPKGVASYAGMQLLDERARSGQSAILTNWGLGLEHWARIVLNIWRE
                     YADDERTLATGEGAWAIQKFSKADLKGGVDVTVDLGMNRPYTQIGRRAVIDQGVRLGV
                     ISPYEPAERFRVLQLLGIPEIMEDYKRERMDASRENDLLMQGLPVRPPMPWENHPVHL
                     ETHKAILTGERFEAMPPEGQQAAEQHAQMHFMNMQQSQAPAGPGQIAPKPPGPNGGNG
                     GTAKGDDGGEADMVHQDAQMASPDMMTGGMPPGGGMMA"
BASE COUNT          499 a          683 c          816 g          398 t
ORIGIN      
        1 gttcgaggaa gatcctgccg tagcggcgga gattcggaaa ctgataacgg agaccctgca
       61 tggccagcac taaacgtccg gcacgcgagg gacccgccag ctacgaggac cacgacggca
      121 tccgggccta cctggaggca gttgaggagt ccgaccggca ggcccgcgag gtccgcgagc
      181 gcgaagcgct ctaccatctg ctgctctaca tcggcttaca gtgggtgacg tgggatcggt
      241 tgctgggtac gtatcgcccg gaggggttga agccgacgac gccgcagccg gtgacgaata
      301 agtttgccgt agggatcaac gccttggttg cctcgacctc agcgttcaat ccgcctctcg
      361 cgtttcatcc ggcgactgag gatgcggacg atattgccgc cgcgaacgtg gccgatcacg
      421 tcctggaggt cgtcaagcac gaggtcaagc accgggattt gcttcagatc gaggcgaagt
      481 ggttgttcct gaccgggaat gtgttccggg tgacgcacta tcaacccgat ctgttgggac
      541 caatgagctt cgtgcagcac gaacgctgcc agatgtgtca acaggtgtcg ccacctgccg
      601 tcctgctgga acagaacaac acctgtccgc actgtgggga acaagggccg tttgtccctg
      661 ctgaggatga agttgggcaa ccgattgggg ataccgtccc gcgtggcgtg atgacgtgtg
      721 aggcccattc ggtgttgacg gtgcgggcgg cctctgaggc ggactccctg gaagagtcgc
      781 cctatgtcgt gattgaccaa cagaagacaa tggagtgggt cgagcgcacc tatggggaag
      841 agatcgcggc gaaggtggac catgagacat cagaatcgcc gtcacggtgg attatgcagg
      901 ccgcgattca ctcgacgggc gctgggttga tgaccggctc cggccagcag gaccagccga
      961 gcccgcaggt ccgggtacgc cgcctctgga tgcgcccaac gccgacgttt ccaggtggac
     1021 tctatgcgga ggtggtgggg aaccaggtcg tccattcggc ggagtggccc taccatgacc
     1081 gcgagggtcg tccgatcctg aacatcacac atacccgctg cgatgtggtg ccggggcgcc
     1141 tgtggggcaa gacacgggcg tcggatgtgg cacagaaaca ggtgcagcgc aataagctcg
     1201 aagcggtgat gacgctcttt gagcggcggt cctcgaacga catcctcatg gtgccgtctg
     1261 gatcggggat tacgaaggtc acagggcaac cgggactgat gttgcagtac aacgccatgg
     1321 ccggggtccc gccgccgcat cgggagccgg gccatccccc gcctccctac ttcatccagc
     1381 ggattcagca gatcgatgag gaaattgacg ccctgtttgg cacgtatgag attttgcgcg
     1441 gcgatgcgcc caaaggcgta gcctcctatg ccgggatgca actgctggat gagcgggcgc
     1501 gatcggggca gtcggccatt cttaccaact gggggttggg gttggagcac tgggcacgca
     1561 tcgtcctcaa tatctggcgg gaatacgccg acgacgaacg cacactggcg accggcgagg
     1621 gggcgtgggc gatccagaag ttcagcaaag ccgatctgaa aggtggcgtg gatgtgacgg
     1681 tggacctcgg catgaaccga ccctatacgc agattgggcg ccgggccgtg atcgatcagg
     1741 gggtgcgatt gggcgtcatc agcccctacg aaccggcgga acgcttccgg gtcctgcaac
     1801 tccttggcat tcctgagatc atggaggact acaagcgcga acggatggac gccagccgag
     1861 agaacgatct gctcatgcag ggcctcccgg ttcgtccacc gatgccgtgg gagaatcatc
     1921 cggtgcactt ggagacccat aaggcgatcc tgaccgggga gcggttcgag gcgatgccac
     1981 cggaaggaca acaggcggca gagcaacacg cgcagatgca cttcatgaac atgcagcagt
     2041 cgcaggcccc ggctgggccg ggacagattg ccccgaaacc cccagggccg aacgggggca
     2101 atggcgggac ggccaaaggg gatgatggtg gcgaggctga catggtgcat caagacgccc
     2161 agatggcgtc accagatatg atgacgggtg gaatgccgcc aggtggcgga atgatggcgt
     2221 aagaccaaca acgcacaggc cggatcgtac ccggcacaag gagtcatgat ggatgagtta
     2281 gagtacccgg acgaatcagc cgagtcgtcc ccggcagacc aggaccccag tcagtacgag
     2341 gagcccgcgg acccccacgc ggctggcggc gaatcgccag acgaggaccc tgacag
//
LOCUS       DATMXI010000197         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_3226, whole genome shotgun sequence.
ACCESSION   DATMXI010000197 DATMXI010000000
VERSION     DATMXI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="msrP"
                     /locus_tag="VN444_03655"
     CDS             complement(<1..657)
                     /gene="msrP"
                     /locus_tag="VN444_03655"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HWR20939.1"
                     /translation="MLIRHAQDIAPSEITDYQLYLNRRSFLIGMAALALSPTRSAVAA
                     PPAGAHLPAARNERFTLEDPQTPLESITTYNNFWEMGPNKDDPARLAHLLKPRPWTLQ
                     IDGHVARPRRYDVEELMRLFPLEERVYRLRCVEGWSMVIPWIGYPLASLIKRVEPTSK
                     ARFVEFTTLHDPAQFPGQRKSLLSFSSLEWPYVEGLRLDEALHPLTLLTFGLYGQVLP
                     N"
     gene            complement(818..1378)
                     /locus_tag="VN444_03660"
     CDS             complement(818..1378)
                     /locus_tag="VN444_03660"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWR20940.1"
                     /translation="MVKKAHALAEQAWKTLSSRPIYQNRWLSLREDLIELPDGRTTIY
                     GVVTCGECAGILPFLNSHTVLLVRQYRYVAKRVTWEMPTGGVHGGESIEAAAQRELNE
                     EIGYQAGRLTRISTYHTSKSIMDETAHLFIGEELARLERPPDETEFIEVRAFPFSDAL
                     QMVLQGDIVDGMTIIAILHAARLRGL"
     gene            1924..>2466
                     /locus_tag="VN444_03665"
     CDS             1924..>2466
                     /locus_tag="VN444_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HWR20941.1"
                     /translation="MAQVEDLPHLLQLPIFQPGEPSLLYDDEGRVLGPIVPEYRAFVP
                     LSRMPLKLRQAIIAAEDARFYEHGAVDLRGMARATIKNLMAASVKEGGSTITQQLTKT
                     LFLSRERTLERKLKELGLAREIEQSYTKDQILEMYLNCIYFGHGAYGVEAAARTYFSK
                     SVTDLTLPEAALLAGLPRAPG"
BASE COUNT          509 a          712 c          735 g          510 t
ORIGIN      
        1 gttcggtaac acctgcccgt acagtccgaa tgtgagcagc gtgagcgggt ggagcgcctc
       61 atccagccgc aacccctcca cgtatggcca ctcgagtgag gagaagctca aaagactttt
      121 gcgctgtccg gggaactgtg ccggatcgtg gagcgtggtg aactcgacga atctggcctt
      181 gctcgtcggt tctacccgtt tgatgaggga agcgagcggg tagccgatcc aggggatcac
      241 catcgaccac ccctcgacac agcgcaggcg atacacccgt tcctccagtg ggaacagtcg
      301 catcagttcc tcgacgtcgt accgcctggg gcgtgccacg tgcccatcga tctgaagcgt
      361 ccatgggcgt ggcttgagca gatgtgccag ccgcgcaggg tcgtccttat tcggacccat
      421 ttcccaaaaa ttattgtagg tagtgatgct ctcaaggggg gtctggggat cctccagagt
      481 gaaccgctcg ttgcgggcgg cagggagatg tgcgccggcg ggcggagccg ccacagccga
      541 tctggtcggg gacagcgcca gggccgccat tccgatcaga aacgaccgcc gattgagata
      601 gagctggtag tccgtaatct ctgatggtgc aatatcttgg gcatgcctga tcaacatacg
      661 attctcctgc aatttcctag aaaacagcgt ttagcactta gcattcagcc gtaggtcggg
      721 ttagcacagc gtaacccgac aacactcggc ttttatggtt gcgtctatgc tctctgtctt
      781 cttagatgct acagagcctc atatggttca gtcgacacta cagacccctc aagcgggcgg
      841 cgtggaggat ggcgatgatc gtcattccgt cgacgatgtc accttgcagg accatctgga
      901 gggcatcgga gaaggggaag gcgcggacct cgatgaactc tgtctcgtcc ggaggccgct
      961 ccagcctggc cagttcctcc ccgatgaaga ggtgggcggt ctcgtccata atactcttgc
     1021 tcgtgtgata ggtgctgatc cgggtaagcc ttcccgcctg atacccgatc tcctcattga
     1081 gctctcgctg ggccgccgcc tcgatcgatt cgcccccgtg gaccccacct gtgggcatct
     1141 cccaggtaac ccgcttcgcg acgtaccggt actgcctcac gagcaggacc gtgtggctgt
     1201 tgaggaaggg gaggataccg gcacactcgc cacaggtcac cacgccgtag atcgtagtcc
     1261 gtccgtcagg gagctcgatg aggtcttcgc ggagggagag ccatctgttc tgatagatcg
     1321 gccggctgga aagggtcttc cacgcctgtt ctgctaacgc atgcgccttc ttcaccatgt
     1381 ctgctccatg agaaatcgcc tcgtgtgctg ggcctgtcct gctctggact gttcgaggga
     1441 gagccgcagg cgtttcaagt tgggtggcgc agggttcacc catcggtgtg gcctcgcgta
     1501 cacgtgtatc gtgacgactg gtctatgtca atgagcaaat agcggccaat ggatctcatc
     1561 aagagatcgc cattccatga tatagtatgg caaggtttgg cgggagatat atggatggat
     1621 tcagacggct ccctgcgtgc aaccctgcga acagtatggg taacggaatc cccgtggcca
     1681 tcagcaggct ggacaaaatg aacaaaagcc caaggtcata agcatgtgca gcgaaaagat
     1741 gaacgagtcc ggaaaggcgg gttctcagga ccacgaatgt cttctttaat gcgcggtgtg
     1801 cctgataggg gggtggtacc agttccctta cggcaagaac gcaaccgtag aataagatgg
     1861 cgggcagcga ccgcgacgct gttcatcacc atgtccctcg tcacggccgc atggggcgcg
     1921 cctttggcgc aagtcgagga cctgcctcac ctgttgcagc tcccgatctt tcagcccggc
     1981 gagccaagcc tcctctacga tgatgaagga agggtattgg gtcccatcgt tccggaatac
     2041 cgggcctttg ttccgctctc ccggatgccc ttgaagttgc gacaggctat catcgccgcc
     2101 gaggacgccc gcttttatga acatggggcg gtcgatcttc gcggcatggc gagggcgacg
     2161 atcaaaaacc tcatggccgc atccgtgaag gaagggggca gcaccatcac gcagcaactc
     2221 acgaagaccc tcttcctgag ccgcgaacga accctggagc ggaagctgaa agaattaggg
     2281 cttgccagag agatcgagca atcctataca aaagaccaga tcctggagat gtacctgaat
     2341 tgtatctact tcggccatgg ggcatacggg gtcgaagccg ccgccaggac ctatttcagc
     2401 aagagcgtca ctgatctcac ccttcctgaa gccgcgcttc tcgctggact cccgcgcgcg
     2461 cccggc
//
LOCUS       DATMXI010000198         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_290902, whole genome shotgun sequence.
ACCESSION   DATMXI010000198 DATMXI010000000
VERSION     DATMXI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290902"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1179)
                     /gene="nuoH"
                     /locus_tag="VN444_03670"
     CDS             complement(100..1179)
                     /gene="nuoH"
                     /locus_tag="VN444_03670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWR20942.1"
                     /translation="MRLAVSFVQILAECSIVMLTVAIMTLAERKVLGWMQDRMGPMEV
                     GPYGILQPLADGVKLFFKEDIVPSGANKAMFALAPMLTMVVSLIGFAVIPFGPNVTLD
                     INGLMIKPFVIADINIGILYILAIASIGAYGIILGGWSSNSKYSLLGGLRSAAQVISY
                     ELNVGLAIVGVLLLSGSLSLVEITEAQAGGFWHWFIWGARPDGFFPIHTQVFAFVVFL
                     ISGVAETNRIPFDLPEAESELVAGFFTEYSGMKFAFFVSAEYASMVLVSCVAASLFLG
                     GWNAPYPGTIFEYVGLEQLAWIEHIMWFAGKVYFFLFVFIWLRATLPRLRYDQLMRFG
                     WKVLLPIALANIIITAITVYFFPRG"
     gene            complement(1244..>2606)
                     /locus_tag="VN444_03675"
     CDS             complement(1244..>2606)
                     /locus_tag="VN444_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWR20943.1"
                     /translation="HFCVAPSQFGAAVLGLLKAVVEGDYVDSSIRQRASAYVSAVAHA
                     VGLLSWDDLALSTGISRESFTQMAVSVARGRRVVVLAGQTLLRSQGSYGICLNLLDLL
                     LLSGKLSEQGCGLAPLAEENNDQGAVEMGAVAELLPGARDLSDQDSRALMTRAWKEEL
                     PPVPGGTLVEMIDRAGAGTIKSLFVIGENPAGSLPPQLRVKEALGKLDLLVCQELFLT
                     ETAALAHVVLPAASAMEKAGTFTNTEGHVQAVRPAIDPVGDSRPDWEILSAFSVFLGA
                     PLEYGEAKEILKEIRSVIPGYGSLGPAPTAPTVDPAAIERYITEGYRRDVAVRYTLPA
                     RTPRQEETVQLGLVQSLFHSGKLSTHSKGLLQIEPSGFLQMNPLDAARFSLSPGDRAC
                     LSNNRGSFTTTVKLFDRVPEGLAWFPDHFAQEALQLFDCVIDPDTKAPSFRTTVVSVK
                     KTT"
BASE COUNT          613 a          774 c          730 g          489 t
ORIGIN      
        1 gtgttggctg cctggtttat gtggttgtgc tggtttgtct agttgggttc gatgtctgcg
       61 ccctctggcc agagaaacga agcaaccaga caacccgttt cagcccctcg gaaagaaata
      121 cacggtgatg gctgtgatga tgatgttggc caaggcgatc ggcaagagaa ccttccagcc
      181 gaaccgcatc aattgatcat agcgcagccg aggcaacgtc gcacgtaacc agataaatac
      241 gaagaggaaa aaatagacct tccccgcaaa ccacatgatg tgctcgatcc acgccagttg
      301 ttctaggccg acatactcaa agatcgtgcc gggatatggc gcattccaac cacccaggaa
      361 cagcgacgcg gccacgcaag acacaagaac catactcgcg tattccgcgc tgacgaagaa
      421 ggcgaacttc ataccgctgt attccgtgaa gaatccggcc acgagttcac tttcggcctc
      481 cggcaaatcg aaggggatac gattggtttc ggccacgccg gaaatcagga acacgacaaa
      541 ggcaaagact tgtgtatgaa tcgggaagaa cccgtcaggg cgcgctcccc agatgaacca
      601 atgccagaag ccgcctgcct gcgcctccgt aatctctacg aggctcaagg atccggacaa
      661 cagcaggacg ccgacgatag ccagcccgac gttcaactcg taactgatca cctgggctgc
      721 ggaacgcaaa cctccgagca gtgaatactt actgttcgat gaccagcctc ccaggataat
      781 gccgtacgcg ccgatagagg cgatcgccag gatgtataag atcccgatgt tgatgtccgc
      841 gatgacaaac ggtttgatca tgagcccatt aatatccagc gtgacgttcg ggccgaaggg
      901 gatgacggcg aatccgatca gggagacaac catggtcagc atcggcgcga gggcgaacat
      961 cgccttgttc gcaccggacg gcacaatgtc ctccttgaag aacagtttga cgccatcggc
     1021 aagcggttgg aggattccgt aaggaccgac ctccatcggg cccatacgat cctgcatcca
     1081 gccaagcacc ttccgctcgg ccagggtcat gatcgcgacc gtcaacatca cgatgctaca
     1141 ctccgccagt atctggacga aggaaactgc aagtcgcaaa cttagctcag tcactgcagc
     1201 actcccggat gacaagtgaa aaatacaatg gcttctcgtc cgttcatgtg gttttcttca
     1261 cggacacaac cgtcgtccta aaggacgggg ctttcgtatc cggatcgatc acacagtcga
     1321 acagttgtag ggcctcctgc gcgaaatgat cggggaacca ggccaacccc tcgggcaccc
     1381 gatcgaacag cttcaccgtg gttgtgaatg agccacggtt gtttgagaga caggcccggt
     1441 ctcccgggct gagagagaat cgggctgcat ccaggggatt catctgcaag aacccgctcg
     1501 gttcgatctg cagcagtccc ttcgaatgag tcgacagttt tcctgaatga aacaggcttt
     1561 ggaccagtcc aagctggacc gtttcctcct gacgcggagt tcgcgcgggc agagtatacc
     1621 gaaccgccac gtcgcggcga tatccctcgg tgatatatcg ttcgatggcg gcaggatcca
     1681 cggtgggagc agtcggcgct ggcccaagcg atccatagcc ggggatcacc gaccggatct
     1741 ccttgaggat ttctttggcc tcgccgtatt ccagcggcgc gccgagaaag actgaaaacg
     1801 ccgagaggat ttcccagtct ggccggctgt ctcccaccgg atcgattgcg ggccgaacgg
     1861 cctggacatg gccctcggta ttggtgaagg tcccggcttt ttccatggcg gacgcagcgg
     1921 gaagcaccac atgggcgagt gccgcggttt ccgtgagaaa gagttcctgg catacaagaa
     1981 gatcgagttt acccagcgcc tctttgactc tgagctgagg cggaaggctc ccggcaggat
     2041 tctctccgat gacgaagagg ctcttgatgg tcccggcgcc tgcccggtcg atcatctcga
     2101 ccagcgtccc tcccggtacc ggggggagtt cctctttcca ggcacgagtc atgagagccc
     2161 gagagtcctg atcagaaagg tctcgagcgc ccggcaacaa ctccgcaacc gcccccattt
     2221 caaccgctcc ctgatcgttg ttttcttccg ccaagggagc caagccgcag ccctgttcgc
     2281 tcaattttcc ggacaagaga agcagatcga gaagattgag gcagatgcca taactgccct
     2341 ggctgcgtaa cagagtttgg ccggcgagca ccacgactcg gcgtcctctt gccacagaga
     2401 cggccatctg ggtgaacgac tcacgcgaga tccccgtgct cagagccaga tcgtcccagg
     2461 aaagcagccc gaccgcgtga gcaacggccg agacgtaggc cgacgcccgt tgacggatgg
     2521 aactatcgac atagtctccc tcgaccacag ccttcagcaa tcccagaacc gccgcaccga
     2581 actgggacgg cgcaacgcag aaatgt
//
LOCUS       DATMXI010000199         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_182182, whole genome shotgun sequence.
ACCESSION   DATMXI010000199 DATMXI010000000
VERSION     DATMXI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182182"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VN444_03680"
     CDS             <1..517
                     /locus_tag="VN444_03680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWR20944.1"
                     /translation="LSSRPIYQNRWLSLREDLVELPDGRTTIYGVVTCGECVGILPFL
                     DSHTVLLVKQYRYVAKRVTWEMPTGGVHGGESIEAAAQRELTEEIGYQAGKLTRISTY
                     HTSKSVMDETAHLFIGEELTKLERPPDETEFIEVRAFPFSNALQMVLQGEIVDGMTII
                     AILHAARLRGF"
     gene            complement(958..1986)
                     /locus_tag="VN444_03685"
     CDS             complement(958..1986)
                     /locus_tag="VN444_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20945.1"
                     /translation="MPDNERLPNWWHTVPGILTAIAGVVTAITGLLLALNQIGVFERK
                     EKAQAPVMRPDGASTSPAKRPPQILQKKPPDTAAKPTPPPVEATFKSGTRRESPRTAQ
                     MDYDAVQALYGKHKRTDDCTEIRRIVAEISEYAGNQQPVPQDARYSVRFPSMKADPTI
                     ADLVTDRITRVRSVKAECFPPQAGFFVREDKVVPQVQVQPSKVPPDSSTLSADRAPTA
                     QKAIQSYGFTFVLDQCKHVRQAVDCQFKIISNQGNRKLIILASSNSVANSGRQFSPEA
                     TVQIGPGKVQIGDTIVFMSGVSRQLKITFTDVPETTDMFAVMQLKLRSQADFFSVQFS
                     DVPIEKGS"
     gene            2220..2603
                     /locus_tag="VN444_03690"
     CDS             2220..2603
                     /locus_tag="VN444_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20946.1"
                     /translation="MVHLIREQVIWHRLNWFRKVQELHSVTDACCFFGISRKTYYKCR
                     NRCTESQGDRLSSFHTSKSVMDETAHLFIGEELTRLERPPDDTEFIEVRAFPFTDALR
                     MVLQGEIVDGMTIIAILHAARLRGL"
BASE COUNT          603 a          747 c          731 g          644 t
ORIGIN      
        1 cctttccagc cggccgatct atcagaaccg atggctctcc ctccgcgaag acctcgtcga
       61 gctccctgac ggacggacca cgatctacgg cgtggtcacc tgcggcgagt gtgtcggcat
      121 cctccccttc ctcgacagcc atacggtcct gctcgtgaag cagtaccggt acgtcgcgaa
      181 gcgggtcacc tgggagatgc cgacaggtgg ggtccatggg ggcgaatcga tcgaggcggc
      241 agcccagcgg gagctcactg aggagatcgg gtatcaggcg gggaagctta cccggatcag
      301 cacctatcac acgagcaaga gtgtgatgga cgagacggcc cacctcttca tcggggagga
      361 gttgactaag ctggagcggc ctccggacga gacagagttc atcgaagtcc gcgccttccc
      421 cttctccaat gccctccaga tggtcctgca aggtgaaatc gtagacggga tgacgatcat
      481 cgccatcctc cacgccgccc gcctgagggg tttctagtgt aggcatttcg aggcggtctg
      541 gggtggaagc tcctcaagct gtggagcgat gaggggggta aaccccatca acttcagtac
      601 ggtgcagggc gtccgcgcta tcgtttagga taagccgctc gggtcacatc gcggcacgcc
      661 ttatatctcc cgtgtgccta cctattacgt agaatggtca gccttgtatc tttatagtgg
      721 agccatgaaa tagcgtagac tcatggtcaa gcacaacccg aacgtgacag accgatcccc
      781 tcttggttgc gagatgccgt ggagtagttg ggtcgtctca tccgtgccgc ttaaggcttc
      841 ttgtacaaga atttgagacg accccaataa agcctcgtga tcgtctcaat ccttattggt
      901 gaggcacagt tggccccagc agaggtacag cgccaagaaa catccattcg taacttctta
      961 actgcccttt tcaatcggga catcgctaaa ctggacactg aagaaatcag cctgggatcg
     1021 taactttagc tgcattactg caaacatatc agttgtctct ggtacatcgg tgaaggtgat
     1081 cttaagctgc ctcgaaactc ctgacataaa aacaatcgta tcgccaatct gtacttttcc
     1141 tggaccaatc tgtactgttg cttctgggct gaattgcctg ccggagttag caacgctatt
     1201 agagctagca agaattatca gcttcctatt gccctgattg cttattattt tgaactgaca
     1261 atccactgct tgcctcacat gcttacattg atcaagaaca aaagtgaatc catagctttg
     1321 tatagctttt tgagcggttg gggctctgtc agcagatagg gttgagctgt ccggcggcac
     1381 ctttgagggt tgaacttgaa cctgagggac tactttatct tccctgacaa aaaacccagc
     1441 ttgcggcggg aagcattcag ccttgacgct ccttacccga gtaatccggt cagtgactag
     1501 gtcggcgatg gtagggtccg ccttcatcga cggaaatctg acactataac gagcatcttg
     1561 tgggaccggc tgttgatttc cagcgtattc actgatctct gccacgatgc gacggatttc
     1621 ggtacagtca tcggtcctct tgtgcttgcc atagagggct tgtaccgcat cgtagtccat
     1681 ctgggcagta cgcggacttt cccggcgagt gccactcttg aacgttgcct ccactggtgg
     1741 gggcgtaggc ttcgccgccg tatctggcgg cttcttttgc agaatttgcg gagggcgttt
     1801 tgccggagaa gttgaagcgc cgtctggccg cataacagga gcttgggctt tctcctttcg
     1861 ctcaaatacc cctatttgat tcagcgcgag aagtagccct gtaatggctg tcacaacccc
     1921 ggcaatagct gtaaggatgc caggaaccgt atgccaccag tttgggagtc tctcgttatc
     1981 aggcacctta cttctccctg tggactgcta tacgcagacc taactattgc atagattggc
     2041 cagcctatct tgcatacaaa aacctccgat ggttgcgctg attctcctgc ctttctaaag
     2101 acttgtcaag gtcctagacg acccgccagt attgttatca tacatgggta acactccctc
     2161 ctgtggaaga agagtggcaa gagtccaacg ctcccaccca accaggaagg aggttactcg
     2221 tggtacacct tatcagggaa caggtcattt ggcatcggtt gaactggttt cgcaaggtcc
     2281 aagaacttca ctctgtcacc gatgcttgct gcttcttcgg gatctcccgg aagacctact
     2341 ataagtgccg caaccgctgt accgagagcc agggggatcg gctgagcagt tttcacacga
     2401 gcaagagcgt gatggacgag acggctcacc tcttcatcgg ggaggagttg accaggctgg
     2461 agcggcctcc ggacgacacc gagttcatcg aggtccgcgc cttccccttc actgacgccc
     2521 tccggatggt cctgcagggt gaaatcgtcg acgggatgac gatcatcgcc atcctccacg
     2581 ccgcccgcct gaggggtctg tagtgtcaac tgaaccatat gaggctctgt agcatctaag
     2641 aagacagaga atatagacgc aaaccatgaa agccgagtat tgtcgggtta cgctgcgcta
     2701 acccgaccta cggctgaatg ctaag
//
LOCUS       DATMXI010000200         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_57547, whole genome shotgun sequence.
ACCESSION   DATMXI010000200 DATMXI010000000
VERSION     DATMXI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57547"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VN444_03695"
     CDS             complement(<1..259)
                     /locus_tag="VN444_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20947.1"
                     /translation="MAKEVISKLFEREIDRVMDPIPDTLDRIAGEFSERGALHSGGYV
                     KAVYDLLVSGLRELTLKRVEIEKEVWRHQAKKPTVADGPTCK"
     gene            complement(402..>2955)
                     /locus_tag="VN444_03700"
     CDS             complement(402..>2955)
                     /locus_tag="VN444_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HWR20948.1"
                     /translation="PPEERSGSLHASEDKLNTHPEWPGLTHRPVAFRELTLERLHERL
                     GELRSRVDDLQTWIDFKALEKRFTEAGLGGFLKGLQEGPPPASQLIDVFHKAIYQAWV
                     SSIVAGDPDLREFRTRHHEQLIEEFREVDRKLVRLSSHRVTEQCNSRRPQRVVMAGDS
                     EVTTLLREANKQRRHLPIRQLSERIPNLLLRLKPCLMMSPISVSQFLNPERLHFDLVI
                     FDEASQICPEDAVGSVYRGKQLIVAGDDQQLPPTAFFQQAMAEEYDWEDISDEEFAVF
                     DSILKECSSIGLPARMLHWHYRSKHESLIAFSNKQFYNNELITFPSAWAKHEHLGIKF
                     VYVPDGVYDRGGKRDNRREAEVVTDLVFEHFARYPEKTLGVVAFSIAQMTAIEDEVEQ
                     RVRSKQEFERFFKDGRLEGFFVKNLENVQGDERDVMIFSIGYGKDQQRRSTMNFGPLN
                     KPGGERRLNVAVTRAREKVMLVSSIRAADIDLSKTQAPGVLQLYRYLDYAERGEAALE
                     LTHPQGGEPESDLESDVAGEIRRLGYDVVHQIGCSGYRIDLGVVDPAEPGRFLLGVEC
                     DGATYHSAYTARDRDRLRQQVLEGVGWRIHRIWSPDWAVRRQTEVERLKRAIEEVRQF
                     ASRGDSLQDSTPSIDQVNNELARTERIEIITPKGGNIPGAVPYKVAGLRPHGRVGRDF
                     HLPQWRQEQCHLLAQLVREEGPVHIDYAVKRIVSAWGLERTGSRIVDAIMEAVTICQE
                     KGSLRRKGKFLWPLNLTKVPIRVPDPNIPESFRDIEHIPVEEIQSALLLIVRHTVGIT
                     CESLLVEAARLFGFDRTGSNIRSILIEVLGKMESSGLVFRNGDSIGLGPMQI"
BASE COUNT          667 a          834 c          732 g          722 t
ORIGIN      
        1 ccttacacgt gggtccatca gcaactgtgg gtttcttggc ctggtgacgc cacacttcct
       61 tttcaatctc gacccgcttc agcgtgagtt ctcgtaaacc tgaaacgagg agatcgtaaa
      121 cggctttcac atatccgccg ctgtgaagtg cgccccgctc actgaattct ccggctattc
      181 tatcgagggt atctggaatc ggatccatga cccgatcaat ttctctctca aacaacttcg
      241 agattacttc tttcgccatt tacacttcct gcaggcaact cagattgcaa atagaagcac
      301 acattcatgt cggggtatta tggtcgatac tatcacgctc ctcggcttgt cacgtgagaa
      361 tagatattga accccccgct cctgacgcta tgcgtacccg ttcatatctg cattggccct
      421 aacccgatgg aatcgccgtt tctgaaaaca agcccgctac tctccatctt tccaagaacc
      481 tcgataagaa tgcttcgaat atttgatccg gtcctgtcga atccaaagag ccgcgcggct
      541 tcaactagga gagattcgca tgtgatgccg accgtatgcc ggacgatcag aagcaacgcg
      601 ctttgaattt cctccacagg aatatgctcg atatcgcgaa acgattctgg gatatttgga
      661 tccggaaccc gaataggaac cttcgtcaga ttgagcggcc acaggaattt ccccttgcgt
      721 ctcaggctgc ccttttcttg gcagatggtt acggcttcca tgatggcatc cacgattcgt
      781 gaaccagtac gttcaagacc ccaagccgat actatccgtt tgacggcata atcgatgtgc
      841 accggaccct cttctctgac taactgtgcc agaagatggc actgctcctg tcgccattgc
      901 ggtaagtgaa aatctcttcc gacacgaccg tgagggcgga gtccagcaac cttgtaaggg
      961 actgccccag gaatgttccc tcccttgggc gttatgattt cgatgcgctc agttctcgct
     1021 aactcattgt tcacctgatc tattgaggga gtagaatctt gaagactgtc cccacgggaa
     1081 gcaaactgtc tcacctcttc gatggctcgc ttcaaacgtt caacttcagt ttgccgtctg
     1141 accgcccaat ctggtgacca gatccgatgg atgcgccagc caacgccttc taatacctgc
     1201 tgtctgagac gatctcggtc cctcgcagta taggctgagt gatatgttgc gccgtcacat
     1261 tcgacaccga ggaggaaacg tcctggctcc gcaggatcga cgacgccgag gtcgatacgg
     1321 taaccactgc aaccgatctg gtgtactaca tcatagccca gccgccgaat ctcaccggcg
     1381 acatccgatt cgaggtccga ctcgggctcg ccaccttgag gatgggtaag ctcaagggcc
     1441 gcttcgcctc tctcggcata gtcgagataa cgatatagct gtaacacgcc gggtgcctgc
     1501 gttttgctca agtcgatgtc agcggcccta atagagctaa ccagcatcac cttctctcgc
     1561 gcacgggtca cagcgacatt caggcgcctc tctcctccag gcttgttgag tggaccgaag
     1621 ttcatcgtgc tacgcctttg ctgatcctta ccatagccga tgctgaaaat catcacgtcg
     1681 cgctcatcgc cctgaacgtt ctcaagattc ttcacgaaaa acccttcaag gcggccatct
     1741 ttaaagaagc gttcgaactc ctgcttggac cgtacacgct gctccacttc atcctctatc
     1801 gctgtcatct gggcaatgct aaacgccacg accccaagcg tcttttcggg gtaacgggca
     1861 aagtgctcaa agaccagatc agtgactact tccgcctctc ggcgattgtc acgctttcca
     1921 ccacggtcat aaaccccgtc aggaacatag acaaacttaa tccccagatg ctcatgtttg
     1981 gcccaagccg agggaaaggt gattagttcg ttgttgtaaa attgtttgtt ggagaacgcg
     2041 atcagcgatt cgtgcttgct gcggtaatgc caatgaagca tcctcgctgg gagtccgata
     2101 gaagaacact ccttgaggat gctatcaaag accgcgaatt cctcgtcgct gatgtcttcc
     2161 cagtcatatt cctcggccat ggcctgctgg aagaaggctg ttggcgggag ctgctggtca
     2221 tcgcccgcga caataagttg ttttccgcgg tacacggagc ccacagcatc ttcagggcag
     2281 atttgcgagg cttcatcgaa gatgacaagg tcgaagtgca ggcgttcagg gtttaagaac
     2341 tggctgacgg aaataggact catcatgagg cagggcttga gcctcagtag cagattggga
     2401 atcctctcag aaagctgacg gatcggtaaa tgtcttcttt gcttattggc ttccctcaac
     2461 agggtagtga cctcggaatc tcctgccatc acgacgcgct gtggtcttcg gctgttacac
     2521 tgctcggtca cgcgatggct tgagaggcga acgagtttcc ggtcgacctc ccgaaactct
     2581 tcgatgagtt gctcatggtg tctggtacgg aattcacgta aatcgggatc tccggcaaca
     2641 atgcttgaaa cccaggcctg atagatagcc ttgtgaaata catcgattag ttgagaggcc
     2701 ggaggcggcc cttcctggag ccccttgagg aagcccccga gaccggcttc ggtaaatcgt
     2761 ttttctaagg ccttaaagtc aatccaggtc tggaggtcgt ccactcggga gcgaagctct
     2821 cccagccgtt catgtaggcg ttcaagagtc aattcacgga acgctaccgg ccgatgagta
     2881 agaccgggcc attcaggatg agtgttcagt ttatcctccg aagcgtgtag agatcccgac
     2941 ctttcctctg gcggc
//
LOCUS       DATMXI010000201         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_241831, whole genome shotgun sequence.
ACCESSION   DATMXI010000201 DATMXI010000000
VERSION     DATMXI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241831"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..938)
                     /locus_tag="VN444_03705"
     CDS             complement(75..938)
                     /locus_tag="VN444_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR20949.1"
                     /translation="MTEIDPIQLIEQDQALKAREIVQGLPSDDPVRVQVETLTGHVAS
                     PEAHQAYYQTMRGGVCPESEVFRRHEQVKRFKKIRELILERKHQTVLDLGCLDGWQLL
                     NLAASGVKGIGVDVCGEAIAVARERALKWGFEVNFLAAPIEEVNLCCGPPGMPIADIS
                     EDTPVTQWLFDAVLMSEVLEHVLDPLTCFKTAVKHLKPNGILYVSVPATAIPHHGKLE
                     DAREHLRVFSEQDILDLAPRLASIGLPITKSSIRRIKASDSPTGRSASGERQSPSIPA
                     TSPEAGTPAIQ"
     gene            complement(935..1750)
                     /locus_tag="VN444_03710"
     CDS             complement(935..1750)
                     /locus_tag="VN444_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR20950.1"
                     /translation="MISTIIPTRNAPSILALTLAHYWANAHDDRLVDSVTLLDNASTA
                     PGMEAVLADAHRRGAHVIRHEKNIGVWASVNRGLALSRSEKVFVLTSDILLAPDALTW
                     LHAIQDDAGVQFLGPKVISDQQEYLWTLYEDPLPAQTVDLSNYNGAAWLFTRDCIEKV
                     GYFDPRFYVCFADVDYTQRVKDAGLSYGVTDAVRCLHLDKCSRRADHTADQDTSIEIR
                     DWERFAEKWAHRPDVMAKHPKPDRIVYNMLKDRYWGEPKETYSNFSTAMEVSA"
     gene            complement(1747..2595)
                     /locus_tag="VN444_03715"
     CDS             complement(1747..2595)
                     /locus_tag="VN444_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWR20951.1"
                     /translation="MDQSNEPIIPHVIIPCAGSGTRFKEQGYQVDKPLIPVGGRRMLD
                     WVLDVIPDVWRNRVITVVRDNQSRLRDELWDWDHGSSVGVKTCIIPGSTQGAACTVLA
                     AAVGLPPDEPVLIMNADQFVKVGTHKMLWAVLADNVRGARHMPDKEIYDDVTGLDGLC
                     KYAFYRWDGFMLTFPGTGPAWSYAVTNGENRVVHVIEKKQVSEHATCGIYFWRKASDL
                     VRSICMMVASHDTTKGEYYLAPSLNHLPLADKDVRIVQVEEFYGLGTPEQVKAFEAKL
                     AEGWRP"
     gene            complement(2728..3396)
                     /locus_tag="VN444_03720"
     CDS             complement(2728..3396)
                     /locus_tag="VN444_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWR20952.1"
                     /translation="MRQRAGRVAFDLDGVIWNSDFSHFEAVNIALAPYGERITEEEHQ
                     TTFKGLPTKRKLAMLTEMGRLPVSAHADVERRKKDATLQTIESVMPRPEVTMLLLALR
                     AAEWQICCCSNSIRDTVQAVMLKMNLLDLMDFTLSNEDVDRAKPAPDIYTKAARIWHI
                     QPEHMVVVEDGEAGKQAARQAGCHLIEVAGPEEVEPWLIHRILEAGRTTTYVHDHTML
                     PVLA"
     gene            complement(3393..3557)
                     /locus_tag="VN444_03725"
     CDS             complement(3393..3557)
                     /locus_tag="VN444_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20953.1"
                     /translation="MKSDIEWVLICPWCDERTIGTWGVREMILSKPCDTPGCQWTWEY
                     IPEPIDMVTA"
BASE COUNT          800 a         1143 c          999 g          883 t
ORIGIN      
        1 gaccgtgacg agatggccct gccgtgccca ggcttcagcg gtctttacga ccagctcttc
       61 cgagccaccg agtgtcattg gatcgctggg gttccagcct ccggtgacgt ggccggtata
      121 gacggtgatt gtcgctcgcc ggaagctgat cgtccggttg gagaatcgct ggccttgatc
      181 ctcctgattg atgatttcgt gatcggcaat ccgatggagg ccaacctggg cgctaagtcg
      241 agtatgtcct gctcgctgaa cacccgcaga tgctctctgg cgtcttcgag tttgccgtga
      301 tgggggatcg ctgtcgccgg aacactgacg tacaggatgc cgttcggctt caagtgtttc
      361 actgccgtct tgaagcaggt cagcgggtca aggacgtgtt caagcacttc cgacatgagg
      421 accgcatcga agagccactg cgtgactggc gtgtcttccg agatgtcagc gatcggcata
      481 cctggaggcc cacagcacag attgacttct tcgatgggcg ctgcgaggaa attcacctca
      541 aacccccact tcagcgccct ttcccttgcc accgcaatcg cctcgccaca cacatcgacg
      601 ccgatgccct tcacgccact ggctgcgaga ttgagcaact gccagccgtc gaggcatcca
      661 aggtcaagca cggtctgatg tttgcgctct aggatcagtt cccgaatctt tttgaaccgc
      721 tttacctgct catgccgcct gaagacctcg ctctcaggac acacaccgcc gcgcatcgtc
      781 tggtagtacg cctgatgggc ctccggcgat gcgacatgcc cggtcaaggt ctcgacctgt
      841 acacgcacag ggtcgtcgga cggcaagcct tgaacgatct cacgcgcctt caaggcttgg
      901 tcctgttcaa tcaattggat cgggtcgatc tcggtcacgc ggacacctcc atagcggttg
      961 aaaagtttga atacgtctct ttcggctcac cccaatatct atccttcagc atgttgtata
     1021 cgatacgatc aggcttcgga tgcttcgcca tcacatcggg ccgatgcgcc cacttctccg
     1081 cgaatcgctc ccagtctcga atttcaatcg aggtatcctg atcggcggta tggtcggcgc
     1141 gacgtgagca cttatcgaga tgcaggcatc gcacggcgtc agtcacgcca tacgacaaac
     1201 ccgcatcttt gacgcgctgt gtgtaatcga catccgcaaa gcacacatag aatctcggat
     1261 cgaaatagcc gactttttcg atgcagtctc gcgtgaacag ccatgccgcc ccgttgtaat
     1321 tcgagaggtc taccgtctgc gccggtagcg ggtcttcata gagcgtccac aaatactcct
     1381 gttggtcact gatgaccttc gggccgagaa actgaacgcc cgcatcatcc tggattgcgt
     1441 gcagccacgt cagcgcatcg ggcgcgagca gaatgtccga ggttaacaca aataccttct
     1501 cggatcggct aagcgcgagt ccgcgattca ctgacgccca gacgccgata ttcttctcgt
     1561 gtcgaatgac gtgagcacca cgccgatgcg cgtcagcgag caccgcctcc ataccgggag
     1621 ccgttgacgc attgtcgagc agcgtcacgg aatcgacaag ccggtcatca tgcgcgttcg
     1681 cccagtagtg cgcgagagtg agcgcgagaa ttgagggagc gttgcgtgtg gggatgatgg
     1741 tggaaatcac ggcctccacc cctctgccag cttcgcctcg aatgccttga cttgttcagg
     1801 tgtaccgagg ccgtagaatt cttcgacctg gacaattcgc acatctttat cagctagagg
     1861 aaggtggttt aggctaggag caagataata ttcgccttta gtagtgtcat gagaagcgac
     1921 catcatacaa atggagcgca caagatcaga ggcttttcgc cagaaatata tcccgcacgt
     1981 tgcgtgttcg cttacctgct tcttctcgat gacgtggaca actcgatttt cgccattcgt
     2041 gacggcgtag ctccatgccg ggccggtccc tggaaatgtg agcatgaaac catcccaccg
     2101 gtagaaggca tatttgcaga gtccatcgag accagtcaca tcatcgtaaa tttctttatc
     2161 tggcatgtga cgtgcgcctc gtacattgtc tgccaataca gcccacagca tcttatgggt
     2221 tccgactttc acgaattgat cggcattcat aatcagcacc ggctcatcgg gaggcaatcc
     2281 tacggctgcg gctaagaccg tacacgccgc gccttgcgtc gatccaggga tgatgcaggt
     2341 tttcacgccg acgctgctgc catgatccca gtcccataat tcgtcgcgca gcctcgactg
     2401 gttatctctg accaccgtaa tgacgcgatt gcgccacaca tctggaatca catcgagcac
     2461 ccaatcgagc atccggcgtc cacctacagg aatcagcggt ttgtctacct gatagccttg
     2521 ttccttgaac cgcgtgccgc tgcctgcgca gggaatgatg acatggggaa tgattggttc
     2581 gttcgactgg tccactatgg gcacctcctg ggtgtgaagg gatcatatga cgacacaaga
     2641 gaatcgaccg ataccgcctt ttggcaatac gggcaatagg tggacgtaat ggctgcagcc
     2701 ttcacgtggc ctgtcaacag gtagatgtca cgccaacact ggcagcatcg tatgatcgtg
     2761 cacataggtc gtcgttctgc cagcctcaag aatccgatga atcagccatg gttcgacttc
     2821 ttccgggcct gcgacctcga tgaggtgaca tcccgcttgc ctcgccgcct gctttccagc
     2881 ttcgccatct tcgacgacaa ccatatgctc cggctggata tgccaaatac gagcggcctt
     2941 cgtgtagatg tccggcgcag gcttggctcg gtcaacatcc tcgttgctca acgtaaagtc
     3001 catgaggtca agtaggttca tcttcagcat gacggcttgt acggtgtctc tgatcgaatt
     3061 cgagcagcag catatctgcc actcagcggc cctgagcgcg aggagaagca tcgtgacttc
     3121 cggtcgtggc atcacagact cgatggtctg taacgtcgca tcctttttgc ggcgctcgac
     3181 atcggcatgg gccgacactg gcaagcgacc catctcggtc aacatcgcca acttgcgctt
     3241 tgtcggcagg cccttgaagg tcgtttgatg ttcttcctcg gtaatccgtt caccgtaggg
     3301 ggccagcgcg atattgacgg cctcaaaatg agaaaaatcg gaattccaga ttacgccgtc
     3361 caagtcgaat gcgactcttc ctgctctctg tctcacgccg tcaccatgtc tatcggttcc
     3421 gggatatatt cccaggtcca ctggcatcct ggcgtgtcgc aaggcttcga cagtatcatc
     3481 tcccggacgc cccatgtccc gatggtgcgc tcgtcgcacc atggacagat caagacccac
     3541 tcgatatcac tcttcatagc ggcttcttga tccaccagat agactctggt gtctcttgat
     3601 taaaccgcca accacgcatg tccatataga aggacacggt acgcttgacc ccgtcttgat
     3661 ttaaatcgtg tccacacagt attccacccg gcttcactcg tttttcccat gccgtgacat
     3721 cagccagtcc cgcatctcga cggtcggaca cgtcacgttc atgtgtgagc caccctcgat
     3781 caggaatgag cgttctatga ccaccccgct cgcatcatca tgccg
//
LOCUS       DATMXI010000202         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_115404, whole genome shotgun sequence.
ACCESSION   DATMXI010000202 DATMXI010000000
VERSION     DATMXI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115404"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VN444_03730"
     CDS             complement(<1..307)
                     /locus_tag="VN444_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWR20954.1"
                     /translation="MEALRQELEDLRAAALDQIQQSTDAAQLERARVHFLGRKASLTA
                     ILRQLVTLPPQERPVIGLLANQIKQAIEARIAAQRAALESIPSDEVLATDQIDISLPG
                     "
     gene            complement(510..869)
                     /gene="rplT"
                     /locus_tag="VN444_03735"
     CDS             complement(510..869)
                     /gene="rplT"
                     /locus_tag="VN444_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWR20955.1"
                     /translation="MPRAKGGFKTRQRRNRVLKEAEGFWGKRSKAYRSAQEAVDRARK
                     YAYRDRKARKRDFRGLWIIRINAAARLSGMSYSALMGGLKKAGVAVDRKALADLAIHD
                     PSTFNKLAETAREQAAA"
     gene            complement(913..1116)
                     /gene="rpmI"
                     /locus_tag="VN444_03740"
     CDS             complement(913..1116)
                     /gene="rpmI"
                     /locus_tag="VN444_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWR20956.1"
                     /translation="MPKIKTLKGAAKRFKVTGTNKVARHKAAKSHLLTGKSRKRKRTL
                     RQSDLVSKADTARMRRLIPYLAK"
     gene            complement(1132..1623)
                     /gene="infC"
                     /locus_tag="VN444_03745"
     CDS             complement(1132..1623)
                     /gene="infC"
                     /locus_tag="VN444_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWR20957.1"
                     /translation="MNDRIRVKEVRVISAEGAQLGILPIQEAIDAAQKLSLDLVEVAP
                     DAKPPVCRIMNYGKYRYEQSKKVREARKKQTVVQIKEIKLRPKTEDHDFQFKAKHAER
                     FLKEGNKAKITMMFRGREMVHIDRGKVLLDRFAEALKEAAMIEQRPRQEGRNMVMILT
                     PKH"
     gene            complement(1634..>3401)
                     /gene="thrS"
                     /locus_tag="VN444_03750"
     CDS             complement(1634..>3401)
                     /gene="thrS"
                     /locus_tag="VN444_03750"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWR20958.1"
                     /translation="LLPGSQFAIGPAIQDGFYYDIEPPRPLTPDDLPVIEAKMRELSE
                     QRLPFARLEVSLEEAIAKATALNQPYKVELLQTIQDRMMACTKAAEQDELAHEVDPAA
                     ERVSFYATGDFIDLCRGPHVLDTSVIRFFRLTHLAGAYWRGDERRPMLTRIYGIAFPT
                     QDALEAHLFLLEEAKRRDHRRLGRELDLFSMDDEVGGGLILWHPKGALVRKLIEDLWR
                     EQHLNNGYDLVSTPHIGRAKLWETSGHLDFYQEYMYPRMEMEGNDYYVKPMNCPFHIK
                     IFQSKVRSYRELPVRFAELGTVYRFERIGVLHGLLRVRGFTQDDAHIFCTPAQMVDEV
                     ARAVNFSVAFLGAFGFDRYDAYIATRPEKAIGDLKLWDDATEALRQAADQAGLSYQID
                     EGGGAFYGPKIDLKVKDALGRSWQCTTVQFDFNLPERFDITYVGEDNRPHRPFMVHRA
                     VLGSLERFFGVLTEHHAGAFPAWLAPVQVRLVPVADRFQPYAQQVSSRLRATGVRTEV
                     DVRNEKVGYKIRDGEMQKIPYILVVGEKESTLGAVSVRKRGGRDLGVMPIEGFVAAIQ
                     EELKPGVKATGLAQHEGGSVHQ"
BASE COUNT          631 a         1141 c          890 g          739 t
ORIGIN      
        1 gccctggaag gctgatgtcg atctggtctg ttgccagcac ctcatcactc gggatcgatt
       61 ccagagccgc ccgctgggct gcaatacgcg cttcaatcgc ttgcttaatc tggttggcca
      121 gcagaccgat cactggccgc tcctgaggcg gcagcgtaac caattgccga aggattgccg
      181 tcaaactggc cttgcggccg agaaaatgca cgcgcgcccg ttcgagctgg gccgcgtccg
      241 tgctctgctg aatctgatcg agagccgccg ctctcagatc ctccagctcc tgccgcagcg
      301 cctccacctc tcctccataa cacaaagccc cgacactggt cggggcatcc cctaggccac
      361 aactacacga tccttcggcc caatactaat cacttgagcc gttcatggtg agcggttcga
      421 caggctcact ataggcgcgt cgaatcacaa tgacccttcg acaggctcaa ggcgaacggt
      481 tgggagggcc tacgtgaaca gcgttgttct taggccgccg cctgctcccg cgcggtctcc
      541 gccaatttgt taaacgtcga cggatcatgg atggccagat cggccaaggc cttcctgtcg
      601 acagccacgc cggccttttt gaggccaccc atcagggcgc tgtacgacat accactcagg
      661 cgcgccgccg cattaatccg tataatccac aggccgcgga agtcccgctt acgagcctta
      721 cggtcacgat aggcgtactt cctcgcccga tcgaccgcct cctgcgcgct cctgtaggcc
      781 ttacttcgct ttccccagaa tccctcggct tccttgagga ccctgttcct gcgttgtctc
      841 gtcttaaatc caccttttgc gcgtggcatc ctattctccc agcctccttg attagccctg
      901 gcccccagcc actcacttcg ccagataggg gatcaggcgc cgcattctgg cagtatccgc
      961 cttagacacc agatccgact gccgcagagt tcgtttccgc tttctcgatt taccggtcag
     1021 caagtggctc ttcgccgctt tgtgacgtgc aactttgttc gttcccgtca ccttgaaccg
     1081 cttggccgct cctttgaggg tcttgatctt cggcaccgtc gctcctctct atcaatgctt
     1141 gggggtgagg atcatcacca tattccgacc ctcctgcctg gggcgctgtt cgatcatggc
     1201 ggcctctttg agcgcctctg cgaagcggtc caagagcacc ttcccgcgat cgatatgaac
     1261 catttcccgt cctcggaaca tcatggttat cttggcctta ttcccctcct tcaagaagcg
     1321 ctcggcgtgc ttggccttaa actgaaaatc gtgatcctcg gtcttgggcc ggagcttgat
     1381 ctctttgatc tggacgaccg tctgcttctt tcttgcctct cgtaccttct tgctctgctc
     1441 gtaccgatac ttcccgtagt tcatgatccg gcagacaggc ggcttcgcgt ccggcgccac
     1501 ttctaccaga tcgagcgaga gtttctgggc agcatcgatg gcttcctgaa tcggcaggat
     1561 gccaagttgc gccccctctg cactgatcac cctcacctct ttgacccgaa tccgatcatt
     1621 cacccgaaca cttctactga tggacgctcc ctccttcatg ttgagccagc ccggtagcct
     1681 tcacgcctgg cttgagttcc tcttggatcg cggctacgaa tccctcgatc ggcatcacgc
     1741 ccaaatctcg cccgccgcgc tttcgcaccg agacggcgcc tagtgtactc tctttttctc
     1801 ctaccacaag aatataggga atcttctgca tttcgccatc tcgaatctta tagccgacct
     1861 tctcgttccg cacgtccact tccgtacgca cccctgtcgc tcttagccgg ctggagacct
     1921 gctgggcgta cggctggaag cgatctgcca ccggtaccag ccggacctgc accggcgcaa
     1981 gccaggccgg gaacgcgccg gcatggtgct cggtcaatac cccaaaaaac cgctccagcg
     2041 atccgagaac cgcgcgatgg accatgaagg gccgatgcgg ccggttatcc tccccgacat
     2101 aggtgatgtc gaagcgttcg ggcaaattga agtcgaactg cactgtcgta cactgccacg
     2161 agcggcccag cgcatctttt accttgagat cgatcttcgg cccatagaag gcgccgcccc
     2221 cttcgtcgat ctgatacgag agccccgcct gatcggctgc ctggcgtaat gcctcggtcg
     2281 cgtcatccca aagcttcagg tccccgatcg ccttctccgg ccgggtggca atgtaggcgt
     2341 cataccgatc aaagccaaag gcgccgagga acgcgacact gaaattgacg gcgcgggcca
     2401 cctcatccac catctgcgcc ggcgtacaga agatgtgcgc gtcgtcttgt gtaaaccccc
     2461 gaacgcgcag gagcccatgc aacacgccaa tgcgctcgaa acgatagacc gttcccaact
     2521 cggcgaaccg caccggcagc tctcggtagc tcctgacctt ggactgaaag atcttgatat
     2581 ggaaggggca gttcatcggc ttgacgtaat agtcgttccc ttccatctcc attctcggat
     2641 acatgtactc ctggtagaag tcaagatggc cgctggtctc ccacaacttc gcccggccga
     2701 tgtgcggcgt agagacgaga tcgtagccgt tgttgaggtg ctgctcacgc cacaggtcct
     2761 caatcagctt ccggaccaac gcccccttag gatgccacag gatcagcccg ccgccaacct
     2821 catcatccat gctgaagagg tccagctcac ggcccagacg tcgatggtcc cgccgtttcg
     2881 cctcctccag gagaaacaga tgcgcctcga gcgcatcctg ggtcggaaag gcgatcccgt
     2941 agattctggt cagcatcggt cgccgctcgt cgccccgcca gtaggcgccg gccagatggg
     3001 tcagcctgaa gaaccggatc accgaggtat ccagcacgtg ggggccacgg cagagatcga
     3061 taaaatcccc ggtggcgtag aagctaaccc gctctgcggc gggatctacc tcatgcgcca
     3121 gttcatcctg ctcggcggct ttcgtgcagg ccatcatccg gtcctggatg gtctgcagga
     3181 gctccacctt gtacggctgg ttcaatgccg tcgctttcgc aatcgcctct tccagcgaca
     3241 cctcaagcct ggcgaacggg agccgctgct ccgacagctc gcgcatcttg gcctcaatga
     3301 ccggcagatc gtccggggtg agcggacgcg gcggctcgat gtcatagtag aagccatcct
     3361 ggatcgccgg accgatggca aattgtgaac cgggcagaag c
//
LOCUS       DATMXI010000203         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_74763, whole genome shotgun sequence.
ACCESSION   DATMXI010000203 DATMXI010000000
VERSION     DATMXI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74763"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN444_03755"
     CDS             <1..180
                     /locus_tag="VN444_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20959.1"
                     /translation="LTRPPDRAPQRLLRADTTPNARGPSTPEVRPILRRLPSSIVVTF
                     SLTGSSSVLYRISQD"
     gene            836..1093
                     /locus_tag="VN444_03760"
     CDS             836..1093
                     /locus_tag="VN444_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20960.1"
                     /translation="MRIGEGNDPDSDSGAIGANPPAYGEPNAGQVIEATWEILPAARF
                     ATIRLMAAAWQGQEEVGGDHRTAKEVDNRTTSEGRIPASTG"
     gene            1279..1995
                     /locus_tag="VN444_03765"
     CDS             1279..1995
                     /locus_tag="VN444_03765"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HWR20961.1"
                     /translation="MGVAMELASHVHWVQVDDVVFVLDVRRGEYFGLDPRTSDLWLRL
                     TRAGGYLPEECDHAALWGIVEAARVRGWLAESGQTVLDDRKDRLGAVRHWRPFLTLSA
                     YMCLVRAFAFLKCLGFSRTYGWAQSIVLASSVRNQNNIDASMLERDLTAFFAAEHVFI
                     SPLGLDDCLPRSLALFVFLRRCGIPVRHLIGVRRYPFRAHAWVEYDGLPLLAPVMYGR
                     TTRLASADLLGDFTPLATIG"
     gene            2106..3794
                     /locus_tag="VN444_03770"
     CDS             2106..3794
                     /locus_tag="VN444_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWR20962.1"
                     /translation="MALVGHLARDPTKDTPATPADRLVRAYRRHGTRLSATLLGQYAA
                     VLVDPLERRLLLVQDSLGIHPLFYTLERGRLVVSANLETLVAFLRPISLNETYFAEYL
                     VNGFPPHQPTPYSCIERLAFGTTLLIEPARRIALQPWSPPMQQEQTRPEDAALRLRTL
                     LDDAVSSTLPAEGRILCELSGGLDSTSVLATAYRQRPDVQALTISSGRGMSGDDESFA
                     GDAVNALGVQWHRIDGDLYPHCSSLPTHFSGEPGNEMKIALQTAYRDVLAREGIDVVL
                     TGSAGDVVFGYGSLRPIHLADALFRGHLLTGIKAARKWSHHAGGQRPWTHYFLHFGVL
                     PAWRHLRRQSLIIHDGDRPPRWLAPDFARRHGFDGSASLQSAPRVSLPGSQYLWEGIY
                     ELAGHASPSFRLHLDADTRHPLFYRPLVEFMLGLDPNLRHGPEGDRVLQRKALVDRLP
                     EAIRTRQTKGSDQQLSEKAQMYNDRWYRALTEDSHLVARGWVNPQRWRAQVERARFGV
                     YNGIASYNAAVMIEWWLRTIECHPPEPSPQLYEPRVPAVQHPAHIRRPALVLSA"
     gene            complement(3961..>5312)
                     /locus_tag="VN444_03775"
     CDS             complement(3961..>5312)
                     /locus_tag="VN444_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HWR20963.1"
                     /translation="TDGLRGRAGHTPTSLAPDLQPLFETILNSLPPPVVEPDGPTQML
                     VNLLAYDDYKGRIAIGRLYAGRLRRTQEVIRIAKDGTYHPDKVAQVFVHQGLDRIEVE
                     EAEAGEIVAVAGLGEIGIGETIADKDNPLALPPLLVDEPTVQMTFSVNTSPFAGREGR
                     WSSSRSLRERLFKELEHNVSLRVHDTESPDSFLVAGRGELHLVVLIETMRREGYELQV
                     SRPEVIFQTDPETGARLEPFERVSIEVPEEQAGLVMEMLGVRRGRLMDMRTGEQGFVH
                     YEFIVPTRGLLGFRQAFLTATRGTGLLNSLFHAYEPMAGEIRARDRGSLVAWEAGVAT
                     AYGLASAEGSGILFIGPGTEAYEGMVVGEAAKERDLEVNVAKRKHLTNMRSSTSDVAV
                     RLTPPRQMSLDNCVEYLAEDELLEVTPKSLRLRKKVLDRHGRKRVRVARNQKPSSVP"
BASE COUNT         1017 a         1644 c         1512 g         1139 t
ORIGIN      
        1 ctgacacgac cgcctgaccg cgctcctcag cgcctgctac gagcagatac cacccccaac
       61 gcccgtggcc cgtccacccc ggaagtccgg cccatccttc gcaggcttcc gtcttccatc
      121 gtcgtcacat tttccttgac aggctcttct tccgtgctgt accgtatctc gcaagactaa
      181 taatggtcct ttatgtcggc tacccccaag cacgacgtga gccgaacccg cgctcaagac
      241 tgagcctaag caggatgagg aaggtgcctg tgcaatgcat ccatgctggc cattgagtcc
      301 taacccccct gatatccgtg tcgaggggaa taataaccct attcaaagga gaatcacatg
      361 aaagaggatc gcttgaaaac agcaccgaag ttgagagcac ttcttggggt tgaccgaccg
      421 gagccgacag cgcatggctc gcccgataac acacccgggg acccgttcat ttctcttggt
      481 acggtggtcg ccttgactgg tcacgacatg catggcaggc agcccgacgg gggaggtaac
      541 ccgggatatg tgtataagga ttgacgtaat aaggaacact aattggtcaa gaggtcatgt
      601 caagaggtgt gcagcgtgcc aggccaaagg tctctatcgc cagcgggtaa aagtcccgtc
      661 cgggtaaccg ccaaagggcc tggaagccag ccccggtcat tcattgagag gtgagtgact
      721 aagcgggatt acaaaccttc ttcgggaagg gggcgtttct gcagtccgta tcataaaggg
      781 aatcctgccg cgtcgtgaat tgaccgccga aaggcgtaag aagaggccaa cccgtgtgcg
      841 catcggcgaa ggcaacgatc ccgactctga ttctggagcg atcggtgcga atcctccggc
      901 gtatggggag ccgaatgcag gacaggtgat agaggcaacc tgggagatcc tccctgctgc
      961 aaggtttgcg actataaggc tgatggctgc agcgtggcaa gggcaggagg aggtcggagg
     1021 ggatcatcgt accgcgaagg aggtggacaa cagaaccacc tcggagggaa ggatccctgc
     1081 ttcaaccggg tgaccgagaa gggggtatgc tggagaaaac cacagctggc taaagactcc
     1141 cacgagaagg tgcgccccct tcagagagag cactgtatca agcggccaag agaagcccga
     1201 agcgttcgat ctcaaccgcc gttgtgtagc attccttcca gccgatcact ctgtagtgcg
     1261 ctgtctggag ggacttgagt gggggtcgcc atggagcttg cctcccatgt gcactgggtg
     1321 caggtggacg atgtcgtatt tgtactggac gtgcgacgcg gcgagtactt cggcctagac
     1381 ccgcgcacgt cggatttatg gctccggctc actcgcgccg gaggctacct acccgaggaa
     1441 tgtgatcacg cagcgctctg ggggatagtg gaggcggctc gtgtccgagg ttggttggct
     1501 gaatctgggc agacagtcct cgatgacaga aaagaccgtt taggcgctgt gcgacattgg
     1561 cggcccttcc tcacgctcag cgcctatatg tgtttggtgc gagctttcgc gttcctcaaa
     1621 tgtctgggct tctcgcggac ctatggttgg gcgcaatcaa tcgtactggc aagttccgtg
     1681 cgaaaccaga ataatataga cgcatccatg ctcgaacggg atcttacggc ctttttcgct
     1741 gcagaacatg tcttcatctc gccgctcggc ctagatgact gccttcctcg gtcgctggca
     1801 ctgtttgtat ttctccgccg ttgcggtatc ccggtccgtc atcttatcgg ggtccggcgt
     1861 tatcccttca gagcccatgc ctgggtagag tatgacggac ttccacttct cgcgccggtg
     1921 atgtatggcc gaactacacg ccttgcatca gccgatcttc tcggcgactt tacgccgctt
     1981 gccactatcg gctagcagtg ggtactagga tcacaaaccc gttctctagc tttctcatga
     2041 tggttaatct ggagacattc accgtccggg gtaaccctcc gcctcagccc gcgcaggggc
     2101 ttttcgtggc gcttgtcggc catttggcga gagatcccac caaagacacc ccagcaacac
     2161 cggctgaccg attggtgcgt gcctaccggc gccacggaac gaggctaagc gctactctgc
     2221 tgggccagta tgctgctgtg ttggttgatc ctttagagcg gcgcttgctg ctcgtgcagg
     2281 attcccttgg catccatcca ctgttctaca cgttggagcg tggtcggctc gtagtctctg
     2341 ccaatttgga aaccttggtg gcgttcttac gtcctataag cctcaacgag acctacttcg
     2401 ctgaatatct cgtcaacggt tttcccccgc accagcctac gccatattcg tgcattgagc
     2461 ggttggcctt cggcactacc ctgttgattg agcccgctcg tcggatagcg cttcagccct
     2521 ggtcaccacc catgcaacag gaacaaacca ggcctgaaga cgctgctttg cggttgcgta
     2581 ctttactcga tgacgccgtt agttccacgc tgccggcgga gggtcgaatt ttgtgcgagc
     2641 tctcgggtgg tctcgattcc actagcgtgc tcgccaccgc atacaggcag cgtccagatg
     2701 ttcaggccct gacgatttcc agtggacggg ggatgtccgg tgacgacgag tcttttgcgg
     2761 gggatgctgt gaacgcactc ggtgtccaat ggcaccggat tgacggtgat ctgtaccctc
     2821 attgttcttc tcttcccacc catttctctg gggagcccgg gaacgagatg aagatcgcct
     2881 tacagacagc ctatcgtgac gtgctggctc gcgaaggtat tgatgtggtt ctgacaggct
     2941 cggctggcga tgtggtgttc ggatatggtt ccttacgtcc aatccatctt gctgatgcgc
     3001 tcttcagggg ccacttattg accggaatca aggcggctcg gaaatggagc caccatgctg
     3061 gcggccagcg cccatggacc cattactttc tgcatttcgg cgtgctgccc gcctggcgtc
     3121 acctgcggcg gcaaagtctc ataatacacg atggcgaccg tccacccaga tggctcgcgc
     3181 ctgatttcgc acgccgccac gggtttgatg gctcagctag ccttcagtct gcaccgaggg
     3241 taagccttcc tggctcgcaa tacctttggg agggcatcta cgaactggcc ggtcatgcaa
     3301 gcccctcctt ccgactgcat ctcgacgccg acacacgtca tccactattc taccgccccc
     3361 tagtcgagtt catgctgggc ctcgatccga accttcgcca tggccctgag ggcgatcgcg
     3421 tcttgcaacg taaggcatta gtcgatcgcc ttcccgaagc tatccgtaca cgtcaaacca
     3481 agggctccga tcagcagctg agtgagaaag cgcagatgta caacgaccgg tggtatcgcg
     3541 cgctaaccga agactcccat cttgtcgccc gcggctgggt caacccgcaa cgctggcgcg
     3601 ctcaggtcga gcgggcacgt ttcggcgtct acaacgggat cgccagttac aatgctgctg
     3661 ttatgatcga gtggtggctt cgcacgatcg aatgtcaccc ccccgaacca tcaccccaac
     3721 tgtatgaacc gcgagtacca gcggtgcagc atccagccca tattagacgc ccagcgttgg
     3781 ttttgtctgc ttgactgagg cgctggattc accaccccct ctagttgggc taggctgcct
     3841 cgggggggaa tcgcttgtgg gccatccctg agagccgctg gtcgggtcta ccgaagctcg
     3901 ggcgcactcg cgagatctcg cgacactgat cgtgccaatc cgttcaggtc cgactgctcg
     3961 ctatggcaca gacgacggct tctggttcct cgcgactctc acccgctttc taccatgcct
     4021 atctaatacc ttcttacgta gccgtagact cttcggtgta acctccagga gctcgtcttc
     4081 tgccagatac tccacgcagt tgtcaaggct catctgcctc ggcggcgtga gacgcactgc
     4141 aacatcggag gtcgaggatc gcatattcgt caggtgcttc cgcttcgcga cattgacctc
     4201 caggtctcgc tccttagccg cctcgcccac gaccatccct tcatacgcct cggtccctgg
     4261 tccgatgaag aggatcccgc tcccctccgc actcgccagg ccgtatgccg tggccacccc
     4321 agcctcccac gcgactagcg acccacggtc gcgcgcgcgg atctcgccgg ccataggttc
     4381 ataggcgtga aacaggctgt tgagcagccc ggtcccgcgc gtcgcggtca ggaacgcctg
     4441 gcgaaatccc agcaaccctc gcgtggggac tatgaactca tagtggacga acccctgttc
     4501 acccgtccgc atatccatca gacgccctcg ccgcaccccc aacatctcca tcaccaaccc
     4561 tgcctgctcc tccggcacct cgatgctgac ccgctcgaac ggctccagcc gcgccccggt
     4621 ctccggatcg gtctgaaaga tgacctctgg cctggagacc tgcagttcat acccctcgcg
     4681 ccgcatcgtt tcgatcagga cgaccagatg taactcgcct cgtccggcca ccaggaagct
     4741 gtcggggctc tcggtgtcgt ggaccctcag actgacattg tgctcgagtt ccttaaacag
     4801 gcgttcgcga aggctgcgac tcgagctcca tcgcccctct cgccccgcaa agggactggt
     4861 gttgacgctg aaggtcatct gcacggtcgg ctcgtctacc aggagcgggg gaagggccag
     4921 agggttatcc ttgtcggcaa tcgtctcccc gatcccaatc tccccaaggc cggccaccgc
     4981 tacgatttcg ccggcctccg cctcctcgac ctcgattcgg tccaacccct gatgcacaaa
     5041 gacctgagcc accttgtcgg ggtggtaggt tccatccttc gcgatccgga tgacctcctg
     5101 ggttcggcgc agacggccgg cgtacaggcg gccgatggcg atccggccct tataatcatc
     5161 gtaggccagg aggttgacca gcatctgcgt gggaccatcc ggctccacca cgggaggagg
     5221 cagggagttg aggatagttt cgaacagggg ctgcaggtca ggagccagtg aggttggcgt
     5281 gtggccggcc cgtccacgga ggccgtccgt at
//
LOCUS       DATMXI010000204         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_241632, whole genome shotgun sequence.
ACCESSION   DATMXI010000204 DATMXI010000000
VERSION     DATMXI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241632"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /gene="pyrF"
                     /locus_tag="VN444_03780"
     CDS             complement(<1..514)
                     /gene="pyrF"
                     /locus_tag="VN444_03780"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWR20964.1"
                     /translation="MQKTPELIVALDVEDLASAAALVEQLYPTVTLFKVGLQLFTAEG
                     PRAVEVVRQRGGAVFLDLKLHDIPNTVAGAVREAARLGAVMCTLHASGGRSMLQAATA
                     AVAASGSPMRLLAVTVLTSLDSRGLEEAVGGKLDLTAQVVRLASLAREAGLDGVVASP
                     HEIGLLRATLG"
     gene            complement(613..1365)
                     /locus_tag="VN444_03785"
     CDS             complement(613..1365)
                     /locus_tag="VN444_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR20965.1"
                     /translation="MQLTILGSGTAIPSLTRGSPGLLVEVAGSSLLFDGGSGTLPRLL
                     KAGISVTELDRVFYTHLHPDHTGDLVPLLFALRNPIWRRTKPLHLTGPQGFLAFYEGL
                     AHLYGDWIAAKTYELVLNETLQDVTDAEGFRVIPREVVHMQYSLCYRIESADGKSLVF
                     SGDTIYCDAMIEAAKGADLLVLECSAPDEQPMKVHLTPTEAGQIAALSGCRRLVLTHL
                     YPICEAYDLVTPCKKVFDGEVILAEDLMRLDV"
     gene            complement(1518..1925)
                     /locus_tag="VN444_03790"
     CDS             complement(1518..1925)
                     /locus_tag="VN444_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20966.1"
                     /translation="MRAWKRLGIGLLTTTMLIGGGMASAQKEQPKEQQKESDPFQNLG
                     LSADQRSKLETAKKEQMSAQNGRQQKLMELRKKLTSLVTDKKASDKEIDQVANQLEIT
                     TREAMKTEMKFFKTLRQILTQEQLTTLSKGGSK"
     gene            complement(2159..3643)
                     /gene="glgA"
                     /locus_tag="VN444_03795"
     CDS             complement(2159..3643)
                     /gene="glgA"
                     /locus_tag="VN444_03795"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWR20967.1"
                     /translation="MKILFAASEAVPFAHTGGLGDVAGALPKALSRLGHDVRLIMPLY
                     RAVEVEQHRLDVVTKGIGIPAASGLQAVDLLEGHLSNGMPVYFVRHDPSFNRTGLYQN
                     PSAEDYPDNAERFALFCRAALEVCRAVSFHPEVLHAHDWQTALLPVYLKTTLRADPFF
                     RQTATVFTIHNLGYQGLFPPEVLPRLLLPLELFTPAGLEFYGKGNLLKGGLLFADLLT
                     TVSRRYSQEIQTAERGFGLDGILRERKSDLFGVLNGIDPEEWNPASDPHIAAHYTVDD
                     LAGKARCKADLQQRFGLPVEATVPLLAVISRLAGQKGLDLLRDNLETLMALNLQLVLL
                     GSGEKELEVAFREAAAKYPSKLAVQIGFDIPLSHQIEAGADLFLMPSRYEPCGLNQMY
                     SLAYGTIPVVHATGGLDDTIVQFDPVTGDGNGFRFRESTATAFLQSIGQALALYPEKA
                     LWPRLITNAMTADFTWDRSAREYEQLYRRAVEKKGEQQRNYSGR"
     gene            complement(3686..3762)
                     /locus_tag="VN444_03800"
     tRNA            complement(3686..3762)
                     /locus_tag="VN444_03800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3726..3728),aa:Asp,seq:gtc)
BASE COUNT          794 a         1182 c         1096 g          777 t
ORIGIN      
        1 gtcccagcgt ggcccgcagc aagccgattt cgtgaggcga agccacaacg ccatccaatc
       61 cggcctctcg cgcgagcgag gcaagacgaa cgacctgcgc cgtgagatca agcttgccgc
      121 cgacggcctc ttcaaggcct cgcgagtcca ggctggtgag gaccgtcacc gccagcagcc
      181 gcatgggcga gccgcttgca gcgacggcgg ccgttgccgc ttgcagcatc gaccgtccgc
      241 cggaggcgtg gagcgtacac attaccgccc cgagccttgc agcctcacgg acggctccgg
      301 cgacggtgtt cgggatgtcg tgcagcttca gatccagaaa gaccgctccg ccccgctgcc
      361 ttaccacctc taccgcccgc ggaccctcag ccgtaaagag ttggaggccc accttgaaga
      421 gtgtcaccgt cggatacagt tgctcaacca gcgcggcggc tgaagccagg tcttccacat
      481 ccagggccac gatcagttcg ggcgtcttct gcatgtcctc gctttactgc ttaggggttt
      541 aggctgaagg ctgaagtatt ctgacagcca tccaccttca gccttcagcc tatctaccta
      601 cacgctgctc ctttacacat ccaggcgcat caggtcttcc gccaggatta cctcaccgtc
      661 aaagaccttc ttgcaggggg tgacgaggtc gtacgcctcg cagatcgggt acaagtgcgt
      721 cagaaccagc cggcggcagc cggacagggc cgcaatctgg ccggcctccg tcggggtcag
      781 gtggaccttc atcggctgct catcgggggc agagcactcc agaacaagca gatccgcccc
      841 cttggccgcc tcgatcatcg catcacagta gatcgtgtcg ccggaaaaaa ccaggctctt
      901 cccatcggcc gactcgatgc ggtagcagag gctatactgc atgtgcacca cctcgcgagg
      961 aatcacccga aacccttcgg cgtccgtaac atcctggagc gtctcattga gcaccaactc
     1021 atacgtcttc gcagcaatcc agtcaccgta cagatgggcc agaccctcgt agaaggctag
     1081 aaatccctga gggcctgtga ggtgcagcgg cttggtccgt cgccagatcg gattcctcag
     1141 agcaaacagg agcgggacca gatcgccggt gtgatccgga tggaggtggg tgtagaagac
     1201 tcggtccagt tcagtaaccg aaatccccgc cttgaggagt ctcggcagcg tgcccgatcc
     1261 gccgtcgaac agaagcgagg aaccggccac ctcgactaag aggccgggtg atcctcgtgt
     1321 gaggcttgga atggcggtcc cggagcctag aatagtgagc tgcatggcgt cacgatacca
     1381 tgaagccatg gcattgacaa gggagggcag gatggagacg gcgcgccaaa catctccagt
     1441 caccgggccg gaggagaatc cgagatcgcc ctagctacgc tacccaagga ccatgctgag
     1501 gagggaatcc ccagcggtta tttgctgccc cctttgctca gagtcgtgag ctgctcctgg
     1561 gtgaggatct gccgtaacgt cttgaaaaac ttcatttccg tcttcatagc ctctctggtg
     1621 gttatctcga gttggttggc cacctggtcg atctccttgt ccgatgcctt cttgtcagtg
     1681 acgaggctcg tcagcttctt ccgaagctcc ataagcttct gctgtcgccc gttctgcgcc
     1741 gacatctgct ccttcttggc cgtctcaagc ttactccgct gatcggccga aagtcctaga
     1801 ttttggaagg ggtctgactc cttttgctgc tcttttggct gctccttttg agctgaagcc
     1861 atgccccctc cgatcagcat cgttgtcgtc agcagtccaa tccccaatct cttccatgcg
     1921 cgcatgtagt tttccccttt ccgttaggcc tgtccccgca tgctttaagc ggggacagga
     1981 ttacccatcg ccaaatgtaa cgactcaggt gtttaggctg taggctgaag gctgttagtc
     2041 gttgcgcatg gaccgaatca aagggcccag aaccttttcg gtctccatca ttgtcgcgtc
     2101 gcattcggaa aacgcctggc tcatctgtgc gcgttcctat agtctaacag cctacagtct
     2161 atctacctga gtagttacgc tgctgttcac ctttcttctc gacagcacgc cgatagagct
     2221 gctcgtattc tcgggcagag cggtcccaag tgaaatcggc agtcatagca ttcgtgatca
     2281 gccgaggcca cagggctttc tcaggataca aagccaaagc ctgcccgatg gactgcagaa
     2341 aggcagtcgc agtcgattcc ctaaacctga atccgttccc atccccagtc acgggatcga
     2401 actggacaat ggtgtcgtcc agcccgcccg tggcgtggac gaccggaatc gttccgtaag
     2461 ccaaactgta catctggttc aggccgcacg gttcataccg cgatggcatc aggaagagat
     2521 cggcgccggc ttcaatctga tgcgacaacg ggatgtcgaa gccgatctgg acggccagct
     2581 tggacggata cttggccgct gcctcacgaa aggctacttc aagctccttc tcaccgctcc
     2641 ctagcaggac gagttgcagg ttcagcgcca tcagcgtctc caggttgtcg cgcagcaggt
     2701 cgagcccctt ctgtccagcc agccgggaga ttacggctaa tagcggcaca gtggcctcga
     2761 ccgggagccc gaatcgttgc tggagatccg ccttacatcg ggcctttccg gcaaggtcat
     2821 caaccgtgta gtgagcagcg atgtgaggat cgctggccgg gttccattcc tcgggatcga
     2881 tcccatttaa tacgccgaag agatcgctct tccgctctct gaggatgccg tccagaccga
     2941 acccacgctc tgcggtctgg atctcctggc tgtagcgacg gcttacggtg gtgagcaggt
     3001 cggcgaagag caacccgcct ttcagcagat tgcccttccc ataaaactcg aggcccgccg
     3061 gcgtgaatag ctccagaggc aacagcaacc ttggcagcac ctcagggggg aagagacctt
     3121 ggtagcccag gttgtgaatg gtgaagacgg tcgcggtctg ccggaaaaag ggatcagccc
     3181 gaagggtagt cttcagatag acagggagca gcgccgtctg ccaatcgtga gcgtgcagaa
     3241 cctctggatg gaaagagacc gcccggcaca cctccaaggc tgcccggcag aacagcgcaa
     3301 accgctccgc attgtccggg taatcctctg cggatgggtt ctgatagagg ccggtacggt
     3361 tgaacgaggg atcgtggcga acaaagtaga ccggcatccc gtttgagagg tgtccctcaa
     3421 gcaggtccac cgcttgcagg ccggaagccg ccggaatccc gatccctttt gtcacgacat
     3481 cgagccggtg ctgctccacc tccacggcgc ggtagagcgg catgatgagc cggacatcgt
     3541 gcccaagccg actgagcgcc tttggcagcg cgccagccac atcgccgagc ccaccggtgt
     3601 gggcaaacgg cacggcctca gaagccgcaa aaagaatttt cacctgggcg atcctgccct
     3661 atgagcggca tgtgatgatg agtagtggcg gggccgacgg gactcgaacc cgcgacctcc
     3721 ggcgtgacag gccggcgttc taaccaactg aactacgacc ccccttttca aaggcgccga
     3781 ataataaccc aataatttgc attgtgcaag aaggattctt atatttcggt ttttactgaa
     3841 tacaaatag
//
LOCUS       DATMXI010000205         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_244444, whole genome shotgun sequence.
ACCESSION   DATMXI010000205 DATMXI010000000
VERSION     DATMXI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244444"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VN444_03805"
     CDS             <1..414
                     /locus_tag="VN444_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWR20968.1"
                     /translation="NHAMPTWVVGKVVEALNRMGKSLNGAKVLVLGVAYKPNIDDQRE
                     SPALEILTLLRQQGAEVNYSDPHVPRCYGHRHYPDLDLTALPLTAETIQAQDVVILAT
                     HHSAFDLELIGRHARLIVDTRNAFKGTPPGKVVRA"
     gene            708..1931
                     /gene="lhgO"
                     /locus_tag="VN444_03810"
     CDS             708..1931
                     /gene="lhgO"
                     /locus_tag="VN444_03810"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HWR20969.1"
                     /translation="MSIQRYDIAIIGGGIVGMATAMALVSRWRPSLIVLEAEDRLAAH
                     QTGHNSGVIHSGLYYKPGSMKARTCVEGREAMFRFCQEHGIPHERCGKLVVATHVSEL
                     PALEELERRGTANGLRGLRRLGPEEIHEHEPHASGVAGLLVPETGIVDYTQVTHALAE
                     LTGKAGATIQTGARVYRIHSNTGELLLETTRGEVRCRYLINCGGLQSDRIARMTGVDP
                     GLQIIPFRGEYYELVPERHSLARNLIYPVPDPRFPFLGVHFTRMIHGGVEAGPNAVLA
                     LRREGYRQWSFAVADTQEMASYAGFWRMARRYWKTGFGECYRSLNKRAFVKALQRLIP
                     ELDLDDVRRAGAGVRAQAVEPSGYLVDDFRIVETERMIHVLNAPSPAATASISIGRTI
                     ANLAAKRFDLPEARV"
     gene            1943..>2574
                     /locus_tag="VN444_03815"
     CDS             1943..>2574
                     /locus_tag="VN444_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20970.1"
                     /translation="MKPIWFDRLLQWLLATLLIFTPLAFGTVEAWSIAVAELLVLSMG
                     VVWIIRMIVEGRIHFEKTSCNLPIILFLILMLFQMVPMPMTVIKHLSPTAYVMYRQAS
                     SLNPEIGWRTISLDPTATRKEFVEVLTYAILFWVILNNFRQRHQIEAIAAIIIGVGVF
                     LAVFGIFQKYYWNGKIYWVRKLPGYSQPFGPYVNRNHFAGYMEMVIPWTMG"
BASE COUNT          542 a          768 c          729 g          535 t
ORIGIN      
        1 aatcacgcca tgcctacctg ggtggtagga aaggtcgtcg aagctctgaa caggatgggt
       61 aagtcgctga acggcgccaa ggtcctggtc ctgggggtgg catacaagcc gaatattgac
      121 gatcaacggg agagcccggc cctggagatc ctgacgttgc ttcggcaaca gggcgctgag
      181 gtgaattact cagatcccca cgtcccacgg tgctatggtc atcgtcacta tcctgatctc
      241 gatctgaccg ccctccccct gacggctgag accatccagg cgcaggatgt ggtcatcctg
      301 gcaacacacc actcggcatt tgaccttgag ttgatcggtc gccacgcccg tctgatcgtc
      361 gatacccgca acgccttcaa gggcacaccc ccaggcaagg tcgtcagggc gtaaccaaca
      421 ggacagagcc gaggtagata tgttgtaaaa gtttctttgc catgttttct catctcaacc
      481 gcgcaattct gctcctcagc cacgagctcg aagacctccg gtggcacatc acatgcaaca
      541 catgatcgag acaatggtag gtcatggaaa ggaccccagt gtccaccaca agcgctcagt
      601 agagtgtatt ctcagagttg gtggccggca gaccatcgca gcgagaacgc catcgcggtt
      661 gtgttcgcgc gcgcgcgccg gttcggcccc atagagcgtg attgaccgtg agcatacagc
      721 gatacgatat tgccatcatc ggtgggggaa tcgtaggcat ggccacggcc atggcgctcg
      781 tgtcgcgatg gcgcccctct cttattgtgc ttgaagcaga ggatcggctg gcggctcacc
      841 agaccggaca caacagtggc gtgatccatt ccggtctcta ctacaagcca ggatcgatga
      901 aggcgcggac ctgcgtcgag ggccgggagg cgatgttccg cttctgccag gagcacggaa
      961 tcccgcatga acggtgcggg aagctcgtcg tcgctacgca tgtcagcgag ctgcctgcct
     1021 tggaagagct ggaacggagg ggcactgcaa acggccttcg agggcttcga agattaggcc
     1081 ctgaggagat ccacgagcac gaaccccatg cgtctggggt tgccgggctg ttggttcccg
     1141 aaacaggcat cgtggactac acccaggtga cccatgcact tgccgagctt accggcaaag
     1201 ccggcgccac catccagacc ggggcacggg tctatcgaat ccattccaac accggcgaat
     1261 tgctcctcga aaccacccgg ggagaagtac gctgtcgcta tctgatcaac tgcggcgggc
     1321 tccaatccga ccgaatcgcg cgaatgaccg gggtcgaccc gggactccag atcatccctt
     1381 tccgcggtga atactacgaa ctggtccctg agcgccactc cctggcgaga aacctcatct
     1441 acccggtccc tgaccccaga ttcccgttcc tcggcgttca cttcacgcgg atgattcacg
     1501 ggggcgttga ggccggaccg aacgctgtcc ttgccttacg gcgtgagggc tatcggcaat
     1561 ggtcctttgc cgtagcagac acccaggaga tggcgtccta cgcggggttc tggcggatgg
     1621 cccggagata ttggaagaca gggtttggtg agtgttaccg ctccctgaac aagcgagcct
     1681 tcgtgaaggc cctgcagcgg ctgattcccg agctcgacct tgacgatgtt cgtcgagcag
     1741 gtgcgggggt tcgcgcacaa gccgtcgagc caagcggcta cctcgtggac gacttccgta
     1801 tcgtcgaaac tgagagaatg atccatgtcc tgaacgcgcc gtctccagcg gcgacagctt
     1861 ctatcagcat tgggcggacc atcgccaatc tggccgcaaa gcgatttgac ctacctgagg
     1921 ctcgggtgtg accgagtcgt gaatgaaacc aatctggttc gatcgtctcc tccaatggct
     1981 gcttgctacg ctcctcatat tcacccctct ggcctttggc accgttgaag cgtggtccat
     2041 cgctgtcgcc gagctcctgg tcctctccat gggggtcgtg tggatcatcc gaatgatcgt
     2101 tgaggggaga atccacttcg agaagacatc atgtaacctc cccatcatcc tattccttat
     2161 actgatgctc tttcagatgg tcccgatgcc catgaccgtg atcaagcatc tctcgcccac
     2221 cgcttacgtg atgtaccgcc aggcatcctc cctcaaccca gagataggct ggcgaacaat
     2281 ttcgctcgat ccaacagcca cccgtaaaga gtttgtcgaa gtcctgacct acgccatact
     2341 cttctgggtt atcctcaata acttccggca acgtcaccag attgaagcca ttgcagcaat
     2401 catcatcggg gtgggcgtgt tcctggcggt gttcggcata ttccaaaagt attattggaa
     2461 cgggaagatc tactgggtcc gtaagctacc aggatacagt cagcctttcg gcccctacgt
     2521 gaacagaaat cacttcgccg ggtatatgga aatggtgatc ccatggacta tggg
//
LOCUS       DATMXI010000206         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_98747, whole genome shotgun sequence.
ACCESSION   DATMXI010000206 DATMXI010000000
VERSION     DATMXI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98747"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..993)
                     /locus_tag="VN444_03820"
     CDS             complement(229..993)
                     /locus_tag="VN444_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20971.1"
                     /translation="MKYLISLCFATLLLVAWTSQARAHGGESMSGMDEADACVLKKGH
                     YTVHFTAYQQQYGASEIGMLYEVGSEKVKKEFKSYCEGIPKTGKFSIAFDLFNEEMRS
                     LPISVRIVKASEGHEDKGSSQHSHDILSLPPTVYRDGAVRLDADIATAGQYMAIMKLE
                     SVGPGIAHKPHSASEPGDLDTVTHSHGSGDPTEAEIHAVDPTFSIPFTVGLETQSRLP
                     SYFSNVGKDAAGVLLGMSVVIGGIIFYRYGMRKKEA"
     gene            complement(1176..1475)
                     /locus_tag="VN444_03825"
     CDS             complement(1176..1475)
                     /locus_tag="VN444_03825"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWR20972.1"
                     /translation="MNQFSLIGFLILAVVIATFSIQNSGEAIVTFIWWQFQSSLVVVI
                     LISTALGAIMAIFLSLPGTFRLRIRLREQSQRIAELEQRLQECETTHTKDIPGTR"
     gene            complement(1472..2812)
                     /locus_tag="VN444_03830"
     CDS             complement(1472..2812)
                     /locus_tag="VN444_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWR20973.1"
                     /translation="MEGLGSGTLVLETVFIFMLILVNGFFAGSEVAIISLRRSRVREL
                     VEAGDGRASTVQRLKDDPDRFLATIQIGINLVSTLASAIGGALAVRVIRPLLDRLPGG
                     GLGATDELVALGIVVVVITYLTIVFGELVPKSLALRYAEQVALAIAGPHEAFGRWFSL
                     IGRPLTASSRLILSLFGAAPQGTTGFVSEAEIKLMVQEGKEKGIFDQTEQELIHSVFE
                     FTETSVKEVMIPRPQIEAIELETPLAEALKLVVDTGYSRYPVYHKSLDDICGVLYYKD
                     LLRLQLENREVSLKAITHPAYFVPETMQVSQLLKELQRRRLSMAIVVDEHGGVDGLVT
                     MEDLLEEIVGEIHDEYEAAEKPVERLRDGSLIIDASLNIKDLRDEHGLLFPESPTYET
                     LAGFMLNQLQRMPKAGDIIIYQDKKLTIVDMDGRRLARIKVEELPATHRAGDVE"
     gene            complement(2924..3694)
                     /locus_tag="VN444_03835"
     CDS             complement(2924..3694)
                     /locus_tag="VN444_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20974.1"
                     /translation="MGWPDVCFLGGGEEDLKRWLEAFEGQVKGVSLPSYNQATPISLK
                     MLSPEERQEVLMALYRGWEVFRKRYDELAARTQGLLRAFFDGGLSPPEALRAPAAFTM
                     AHNLEESLRQWLSDGGRGLYRSLLLLADEAKRLDLLQQEQLQDFLSMAFGARVRRLRE
                     CPDLNRLRGVQEVVDIADRMGYALDQLEFVILMYELLTHDVPPLIERVLQTESREECN
                     LVSAVLRLGERFGFSVGRLRQRLRPIEEQLAADPGLWP"
     gene            complement(3624..>4307)
                     /locus_tag="VN444_03840"
     CDS             complement(3624..>4307)
                     /locus_tag="VN444_03840"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWR20975.1"
                     /translation="QADRLFTDVWAARDGYIALLLDRSNSSIEEFFGAHARRSLSLQD
                     RVAALQLLEMQRHALLMQSSDGWFFSDISDIEAAQNLKHAARALELASAFVAVNVEAK
                     FLARLQTAKSNIPSERDGRRVWEVRVRTARVDLTHPAALYAMRSLVENLSEPYRVYTF
                     DLHRLSLERFPLINGTMIAGGVEVCSSFTLEREEFGFVLKWLDTDGMAGCLFPWGGGG
                     GSEAMAGGV"
BASE COUNT          920 a         1295 c         1189 g          903 t
ORIGIN      
        1 ctacctgtgt agtgagaaag cgagagaggg aagccgtagc ttacaatatg gcggattcct
       61 caaaaagcct aaacgccatg aagactagca acctagcctt cagtctgtca agggaaaacc
      121 tgggaaatca ctgaaaacgg actaggcatg gcaccaccct gtaacgagct tgggggaatg
      181 ggcctgacgt ccgttggagg gtaagccggg taagcctgtg tacccctttt aggcttcctt
      241 tttccgcatg ccgtatctgt agaatattat gccgcctatg acgaccgaca tgccgagtaa
      301 tacgccggct gcatccttcc ccacgtttga gaaatacgag gggaggcgcg actgtgtttc
      361 gagacctacc gtaaagggaa tgctgaatgt cggatcaacg gcgtgtattt ctgcctctgt
      421 tgggtcaccg ctaccgtgac tatgcgtgac cgtgtccagg tccccaggtt cggaagcgga
      481 atgaggctta tgggcaatcc caggcccgac actttccaac ttcataatgg ccatatactg
      541 accggccgtg gcaatgtcgg catcgagtct gaccgcccca tcacggtaca ctgtcggcgg
      601 cagggagagg atgtcatggg agtgttgact gctgcccttg tcctcgtggc cttcggaggc
      661 cttaacgatc cggacggaga tgggcaggct tcgcatctct tcattgaaca ggtcaaaggc
      721 tatcgagaat tttcccgtct tcgggatccc ttcgcagtac gacttaaatt ccttttttac
      781 cttttcggac cctacttcat acaacatgcc gatctctgat gcgccgtatt gttgctgata
      841 ggccgtaaag tgaaccgtgt agtggccctt ctttaggacg catgcatccg cttcgtccat
      901 gccactcatc gactcaccgc cgtgggcacg ggcctgggat gtccaggcaa caagcagaag
      961 cgtcgcgaag cataacgaga tcaggtattt catgtttcaa ttcctccttc agttgtaact
     1021 aagatttcca tgttgcgtga tccagcaata agtattatat accaagttac cttccacttt
     1081 ccacttttgg ggagtcctgt ttacggtcta gacgctcccg gcacctaccc tggaacgcct
     1141 tcggcaagaa acccgagaca gagattcccg gtcgatcacc gagttccggg tatgtccttg
     1201 gtgtgtgtcg tttcgcattc ctggagccgc tgctccagct cagcgattcg ctgtgactgc
     1261 tcccgtaatc gtattcgcag ccggaaggtt ccgggaaggc tcaggaagat cgccatgatc
     1321 gctccgaggg cggttgaaat caggatgacg accaccagcg aactctggaa ctgccaccag
     1381 atgaaggtga cgatcgcctc gcccgagttc tgaatggaaa aggttgcgat gaccactgcg
     1441 agaatcagga agcctatgag ggagaactga ttcattcaac gtctcctgct cgatgcgttg
     1501 ccggcagctc ttcgaccttg attcgggcga gccgtcggcc gtccatgtcc acgattgtca
     1561 gcttcttgtc ttgatagatg atgatatctc cggctttggg cattcgctga agttgattca
     1621 gcataaaacc cgccagggtc tcgtaggtag gcgactcagg gaatagtaac ccatgctcgt
     1681 cccgcaggtc cttgatattc agagaagcgt cgatgatgag cgagccgtcc ctcagccgtt
     1741 cgacgggctt ctcggcagcc tcgtactcgt cgtggatctc tccgacaatc tcttcgagga
     1801 gatcctccat ggtgaccagc ccgtccacgc ccccatgctc atccaccacg atcgccatcg
     1861 agagccgccg ccgttgcagc tccttcagga gttgactgac ctgcatcgtc tccgggacga
     1921 agtaggcggg gtgcgtgatg gccttgaggg agacctcgcg gttctccagt tgtagccgca
     1981 gcaggtcctt gtagtatagg acgccgcaga tgtcgtcaag actcttgtgg tagacggggt
     2041 agcgagagta gcccgtgtcc accacgagct tgagtgcctc tgccagtggg gtctccagct
     2101 caatcgcctc aatctggggg cgagggatca tgacctcctt gaccgaggtc tccgtaaatt
     2161 cgaagacact gtggatcagc tcctgctctg tctggtcgaa gatccccttc tccttcccct
     2221 cctgaaccat cagcttgatc tctgcctcgc tgacaaaccc tgttgtcccc tgaggggcag
     2281 cgccgaagag cgagaggatc aggcgacttg acgcggtcag tggacgaccg atcaacgaga
     2341 accaacggcc aaaggcctcg tgaggtccgg cgatcgcgag ggcaacctgt tccgcataac
     2401 ggagggcaag cgatttgggg accagctctc cgaagacgat cgtgagatag gtgatgacaa
     2461 cgaccacgat cccaagggcg accaactcgt ccgttgcccc cagtcctccc cctggaaggc
     2521 gatcaagcaa ggggcggatc accctcactg ccagcgcccc gccaatagcg gaggccagcg
     2581 tactgacaag attgatcccg atctggatgg tagccagaaa tcggtcgggg tcatccttga
     2641 gtcgctgaac tgtcgatgcc cggccatctc ctgcttccac aagctcccgt acccggcttc
     2701 gccggagcga gatgatcgcg acctcagagc ccgcgaagaa gccgttgacc aggatcagca
     2761 tgaagatgaa caccgtctcc agcaccagtg ttccactgcc taagccttcc atcagattac
     2821 tccttgctta gccatcagcc gtcagtgtag gggcagggct tgccctgccc gatgagggcg
     2881 cagcaagcgg cgcccctacg ttgctgaccg ccgatagccg cctttaaggc cagaggccgg
     2941 gatccgccgc cagttgctct tcgatcggac gaaggcgctg ccgaagcctt ccgacagaaa
     3001 acccaaaccg ttcgcctaga cgaagaacgg ctgagaccag gttacactcc tccctggact
     3061 cagtctgaag tacccgctcg atcagcggcg gcacatcgtg cgtaagaagt tcatacatca
     3121 agatgacgaa ttccagttgg tccagggcat agcccatccg gtcagcgatg tcgacaacct
     3181 cctgtacccc ccggagtcta ttcaggtccg gacactcgcg caaccgcctc acccgcgccc
     3241 cgaatgccat ggacaggaaa tcctgcaact gctcctgctg cagcaggtcg agtcgcttcg
     3301 cctcatcggc cagcaggagc aggctgcggt acagacctct cccgccatcg gacagccact
     3361 gccgtaggga ctcctccagg ttgtgggcca tggtgaacgc agccggggct ctcagtgcct
     3421 cagggggaga gagtcccccg tcgaagaacg cgcggagtaa cccctgggtt cgggccgcca
     3481 gttcatcgta gcgtttgcgg aacacttccc accctctgta gagcgccatt aagacctcct
     3541 gtcgctcttc cggggaaagc atctttaatg aaatcggcgt cgcctggttg taactcggca
     3601 gcgatacgcc tttcacctgc ccctcaaacg cctccagcca tcgcttcaga tcctcctccc
     3661 cccccccaag gaaacagaca tccggccatc ccatcggtat ccaaccactt caggacaaac
     3721 ccgaactcct cgcgctccag ggtgaaggat gaacatacct cgacgccgcc ggcgatcatc
     3781 gttccattga taaggggaaa gcgctcaaga ctcagccgat ggaggtcaaa ggtgtacacg
     3841 cgatagggct cagacaggtt ctctaccagc gatcgcatgg cgtacagggc tgcggggtga
     3901 gtgaggtcga cccgtgctgt tcgaactcgg acctcccaga cgcgccggcc gtcgcgctcg
     3961 gaaggaatat tactcttcgc ggtctgaagc ctggccagga atttcgcctc cacgttcaca
     4021 gcgacaaagg cagacgcgag ttcgagggcc cgcgctgcgt gtttaagatt ctgcgcggcc
     4081 tcgatgtcag aaatgtcgct aaagaaccaa ccgtcactgg attgcatcag gagggcatgc
     4141 cgttgcatct ccagtagttg tagtgccgcc actctgtcct gtagcgagag tgatcggcgg
     4201 gcgtgcgccc caaagaactc ctcgatcgag gagttgctcc gatcaaggag caaggcaatg
     4261 taaccgtccc tcgcggccca gacatcggtg aacaaccgat ctgcctg
//
LOCUS       DATMXI010000207         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_195417, whole genome shotgun sequence.
ACCESSION   DATMXI010000207 DATMXI010000000
VERSION     DATMXI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195417"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VN444_03845"
     CDS             <1..1906
                     /locus_tag="VN444_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWR20976.1"
                     /translation="SYSTGANLSTLRNTLSTDLRRHRIGFGETGTSGPFPNTALNAIL
                     QQQPRTQASWNMTDATLQELVRGYGSAKLYAQHADEPQSQAEIDAINDPNDEVQRYRR
                     NGVPELLTVSWSREGDYIGWQANVDIFAAVEFALIPFTKAREKLNAGKHVWSYVAGIQ
                     PNEAPNWLLDYDLINQRIVPWLDYSTGLTGFLYWTPVNWCPGDPWTNAGSATNPCNID
                     TNRNLDGVFYFPGHKVGAPNAAIPSARLKAIRDGVEDYDYLALLKSLGDPTLAQTLAQ
                     TLAPAWDSWNHDPAALLAAREQAAGRILHLSGTSSSSSDAIPPTISAVAVSGLTATSA
                     TVTWTTSEAADTQVEYGPTTSYGTVSPLDGARVTSHTVTLTGLNAGTLYHYRVKSRDA
                     AGNLAVAADATFTPAGATPAANLVGYWPFEDGWGTLAADASGTGNPGTLLNGLAWRSG
                     QVGGALAFDGVNDAVVVADSPSLDTLGAAFTVAAWVQGTESDDGVDDDRAILGKGDTG
                     SAFKNTFTFGLDNAKRLTLRIANGSSYNRVYGATSLQPHVWYHVVGTFDGTQLNVYVN
                     GVRDAAPFTTTLSGSLINSGNLVLGRQSAADCASPYNACWKGLLDEVRVYTRALSATE
                     VQALYNEFSF"
BASE COUNT          406 a          680 c          652 g          383 t
ORIGIN      
        1 ctcctactcc acgggggcga acctcagcac gctcaggaat acgctctcca ccgatttgcg
       61 acggcatcgg atcggcttcg gggagacggg gaccagcggc cccttcccga acaccgccct
      121 caatgccatc ctgcagcagc agccacgaac ccaggcatct tggaacatga ccgacgcgac
      181 cctccaggaa cttgtgaggg ggtacggctc ggcaaagctt tacgcccagc acgcggatga
      241 gccacaaagc caagcagaga tcgatgcgat caacgatccg aacgatgagg tgcagcggta
      301 ccggcggaat ggcgtcccag agttactgac cgtatcctgg tcgcgtgagg gtgattatat
      361 cggctggcag gccaatgtcg atatcttcgc ggccgtcgag tttgcgttga ttccgtttac
      421 caaggcgcgg gaaaaactta acgctggtaa gcacgtctgg agctacgttg caggcattca
      481 gccgaacgag gccccgaact ggttgctgga ctatgatctc atcaatcagc gcatcgtccc
      541 gtggctcgac tattcgacgg gtctcaccgg cttcctgtac tggacccccg tcaactggtg
      601 tcctggcgat ccgtggacca acgctggaag tgcaacaaat ccgtgcaata tcgacaccaa
      661 ccgcaacctg gacggggtct tctacttccc cggccacaag gtgggcgccc ccaacgcggc
      721 catcccctcg gcccgcctga aggcgattcg ggatggggtg gaggattacg actacctggc
      781 gctgctcaag agtctcggcg acccgacgct ggcccagacg ctcgcccaga cgctcgcgcc
      841 ggcatgggat tcctggaacc atgatccggc ggcactcctc gcggcgcgcg agcaggcggc
      901 ggggcggatc ctgcacctga gcgggacctc gtcctcctcg tccgatgcca ttcccccgac
      961 gatctcggcg gtggcggtct ccgggcttac cgccaccagc gccacagtga cctggacgac
     1021 cagtgaagct gctgatacgc aggtggagta tgggccgaca accagctacg gtacggtgag
     1081 tcccctggat ggcgctcgag tcacgagcca tacggtcaca ctcacagggc tcaacgccgg
     1141 gacgctgtac cactatcgcg tgaagtcgcg ggatgccgcc ggcaacctgg cggtcgctgc
     1201 ggatgcgacg ttcacccccg ctggggcgac ccccgcagcc aacctcgtcg gctactggcc
     1261 gtttgaggac ggctggggaa ccctcgccgc cgatgcctcc ggcaccggca atcccggtac
     1321 gctcctcaat ggcttggcgt ggcggagcgg gcaggtgggc ggggcactcg cctttgatgg
     1381 cgtcaacgac gcggtcgtcg tcgcggattc gcccagcctc gatacgctcg gggcggcctt
     1441 cacggtcgcg gcctgggtcc aggggaccga gtcggacgat ggggtcgatg atgaccgcgc
     1501 tattctcggg aagggggata cggggtccgc gttcaagaac accttcacgt tcgggctgga
     1561 taacgccaag cggctcaccc tgcggatcgc caatggcagc tcgtacaacc gcgtctacgg
     1621 ggccacgtcc ctgcaacccc atgtctggta tcacgtggtg gggaccttcg acgggaccca
     1681 actgaacgtc tacgtcaatg gggtgcggga tgcggcgccg ttcaccacga cgctcagcgg
     1741 cagcctgatc aactccggca atctcgtcct cgggcggcag tcggccgcgg actgcgccag
     1801 cccgtacaat gcctgttgga aggggctgct cgatgaggtc cgcgtgtaca cccgggcgct
     1861 ctccgcgacg gaggtgcagg ccctgtataa cgagtttagt ttctaagctc caatcagagc
     1921 actgcgtgac cacgccaggc atgctgcacc cttcagtcag caaggagtgg tcacgcagtg
     1981 ctgacttacg gagcataata acatagtaac agatacgatc tcactgtatt gactatggaa
     2041 catcgtttta caccgcatta agtaattttg tctcatgtcc gccagttagt cgagcacata
     2101 ttacgcaatc cctcgcggtg c
//
LOCUS       DATMXI010000208         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_117928, whole genome shotgun sequence.
ACCESSION   DATMXI010000208 DATMXI010000000
VERSION     DATMXI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117928"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VN444_03850"
     CDS             <1..160
                     /locus_tag="VN444_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008308577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWR20977.1"
                     /translation="ELYGEVFEPLKDPEFFKQVAVNGETNTIEWPNGADFAPEFLYKI
                     GEQIKQVA"
     gene            367..588
                     /locus_tag="VN444_03855"
     CDS             367..588
                     /locus_tag="VN444_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HWR20978.1"
                     /translation="MIKELDTVVLTEDLSEHDLQRGDLGTVVLVHPAGGYEVEFVTLN
                     GETFTVVSLFPHQVRTAGRREIAHARLVA"
     gene            1037..1258
                     /locus_tag="VN444_03860"
     CDS             1037..1258
                     /locus_tag="VN444_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HWR20979.1"
                     /translation="MAKTVDKLTAEIHDLPDLEKLRLVDAILTDLDRPDPEIDRVWAE
                     EARKRWAAYKAGRVPTVSYETVMSKHRPS"
     gene            1268..1435
                     /locus_tag="VN444_03865"
     CDS             1268..1435
                     /locus_tag="VN444_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HWR20980.1"
                     /translation="MFAHNHPSGELQPSDADLRMHKQLTEAGKILGLRVLDHVIVTRK
                     GYFSFQEAGLI"
     gene            1766..1948
                     /locus_tag="VN444_03870"
     CDS             1766..1948
                     /locus_tag="VN444_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20981.1"
                     /translation="MSGSTTKQKALEAIETLPADATLEDAIERLVLLAKIERGLAELD
                     AGKGVGHTEATRRLRR"
     gene            2037..2231
                     /locus_tag="VN444_03875"
     CDS             2037..2231
                     /locus_tag="VN444_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR20982.1"
                     /translation="MVERIVAAVARLENHPLFGRVVPEVGDESLREVIHGNYRIVYRP
                     KHDIVEIVTVFHGARLFRAD"
     gene            2391..>2671
                     /locus_tag="VN444_03880"
     CDS             2391..>2671
                     /locus_tag="VN444_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20983.1"
                     /translation="MRLIGKSISGRRAIAIGTAFLLWTVLWQQSAFGGEPTAKPIQSK
                     VAAKIDDHVITLDEVEKALAPQLAKLEDQKFQLLESKLEELIEKRLLAV"
BASE COUNT          628 a          697 c          794 g          552 t
ORIGIN      
        1 ggaactctac ggagaggtgt tcgagcccct gaaggatccg gagtttttca aacaggtagc
       61 cgtaaatggg gagacgaaca ctatcgagtg gcccaatggg gcagattttg cccctgagtt
      121 tttgtacaag atcggcgaac agatcaagca agtcgcttaa gcatctgcgc tgagggttac
      181 ggtccaacaa agcaatgaag ccttcggtct ccactggcgc tcgggggctg ccgatgatta
      241 tgtggtcgaa gaacgatatc aacttcgggt ggccggatgt taaacgtgca caccgtgtgg
      301 ttcgttgaga cagggcatag tacaccccgg ttcgtgaccg cgtatccccc ttgaaaggag
      361 ataccgatga ttaaagaact ggacaccgta gtgttgaccg aagacctatc ggaacacgac
      421 ctgcagcgcg gcgatctggg gaccgttgtc ctggtccacc ctgcgggagg ttatgaggtc
      481 gaattcgtaa cgctgaacgg tgagacattc actgtggtgt cgctgttccc acatcaggtg
      541 cggaccgcag gccgacgcga aattgcgcac gcacgcttgg ttgcgtaacc caccccgaca
      601 acctcttgcc cactcctcca ggacgtggtt ttctcagcat cccctcccgt cgccctcacc
      661 tcctagggag gaaggtcaag acagagggtg aaggatcctt ttcgtgcgtc gtggtacggt
      721 gtagcctcgt ggggtgtggc tcaagggaac agaaaatacc tggacctggg tactgaggag
      781 acatgcatcg cctacgtcga gcgcggtggc gagacggacg agattctgaa aaggcttaac
      841 gaatggtagg tcttaattct gtgagccaca ggcccaaact cgcgaatctc cgaacctcaa
      901 cctgagactg aaaagaccgt agaaggccga cctcagatcg aaacaacctc ctctcatgtc
      961 ccttaagctt ccaaaagaca tctcctgcga cgagcgtcac gctgcttgga tccgcgtggt
     1021 cgagcggatc gctgatatgg ctaagacagt ggacaaactc acggcggaga ttcatgatct
     1081 gccggacctg gagaaattgc ggctggtgga cgccatcctg accgatttgg acaggccgga
     1141 cccagagatc gaccgagtgt gggcggaaga ggcgcgcaaa cgctgggcgg cctataaagc
     1201 gggccgagtc ccaacggtat cctatgagac cgttatgtcg aagcatcgcc cctcatgaaa
     1261 gtccactatc ttcgcacaca accacccatc cggcgaactt caacccagcg acgccgatct
     1321 tcgcatgcac aagcagttga ccgaggccgg aaagattctc ggacttcgtg tcctggacca
     1381 tgtgatcgtg acgcggaaag gctacttcag ctttcaggaa gccggcttga tttgatgaga
     1441 gggctcggcg aagacaacag gtcaaggatc caggccaacg aatcatcgtt gccagcggat
     1501 tggctggtct gccttcacgt tatgcttgaa gtggcgatgc gacctgcatg ccactggcac
     1561 ggcgctgaac cgcggaatat actggcgggc catgtgagga tggcgggata gagattcagc
     1621 cgacagacgt gtgtcgtgat atactgactc gcagatggag gatgtccgac ggcacagtaa
     1681 ttgcgttgtt aggcactgag gtagagcgcc tactgtgtta gcgctactta agctagcaat
     1741 gtggagcgca gcgagggagg catcgatgtc aggaagcacc accaaacaga aggcccttga
     1801 ggcgattgag acgctgcccg cagatgccac gctggaagat gctatcgagc ggctagttct
     1861 cttagcgaaa atcgagcggg ggttggcgga actggacgcc ggaaagggcg tcggccacac
     1921 tgaagccacg cgccgacttc gccggtgact cggatcatct gggcacccca ggcgatcgag
     1981 gacgtcgagg cgatacgggc ctatgtggcc gcgactcctc tcactacgcc gacctggtgg
     2041 tagagcggat cgttgcagca gtggccagac tcgagaatca ccctctgttt ggacgagtgg
     2101 tccctgaggt tggtgacgag tcgctccgcg aggtcattca cggtaactac cgaatcgtgt
     2161 atcgcccaaa gcacgacatt gtcgagattg tcaccgtgtt tcacggtgcc cgattgtttc
     2221 gagccgacta aggcaagcac ctattaaagt attgcttgaa tgtaaagtgc gtaatatctc
     2281 attagaccag ctttctgcgc acgctatata gttggtgttt gttttccgac acttactagt
     2341 acatcatcgg tttcgtctga caccaatgac aaaatgacaa ggaggcaata atgcggttaa
     2401 tcggaaagag catctctgga cgccgggcta tcgcgatcgg tacggccttc ctgttgtgga
     2461 cagtgctgtg gcagcagtcc gcgtttggcg gcgagccgac ggccaaaccg attcaatcga
     2521 aggtggctgc aaagatagat gaccatgtga ttacgcttga tgaggtcgag aaggctctgg
     2581 ccccgcagct tgccaaattg gaagatcaaa agttccagct cctggagtca aagctggagg
     2641 agctgatcga aaagcgcctt ctggcagtcg a
//
LOCUS       DATMXI010000209         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_33416, whole genome shotgun sequence.
ACCESSION   DATMXI010000209 DATMXI010000000
VERSION     DATMXI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33416"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..653)
                     /locus_tag="VN444_03885"
     CDS             complement(159..653)
                     /locus_tag="VN444_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWR20984.1"
                     /translation="MSPVELMVLTQPELNEIFAHAEEAYPDEACGVVIGKPGDPNTNI
                     VRRCANLANQYQRDDPIRYPRDAKTAYIMDPKDLLKIQSEADAKGLEFVLIYHSHPEH
                     EAYFSETDRDLALFDGEPLWPQLRYLVVSVKNGRVSYFKVFRWDSIVKQFTEESSAVF
                     ERGG"
     gene            complement(661..1596)
                     /locus_tag="VN444_03890"
     CDS             complement(661..1596)
                     /locus_tag="VN444_03890"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWR20985.1"
                     /translation="MKSVDILDAIGHTPLIELPRMSPKKEVRIFAKLEGVNPTGSLKD
                     RIAKYMIEQAERSGELTKDKTILEPTSGNTGIALAMIGRRKGYKVKAVIPENATPERR
                     QLLEIFGAELIYSDGTKGSNGAIELAQKLVAQDPTLYMPFQYGNPANPLAHYETTGVE
                     ILNDLPEVDVLVAGLGTGGTLMGVGRRLKECNPKTKIIAVEPNPGDLVQGLRSLEEGF
                     IPPILDASLLDGKIMVDSRCAFAATKDLTNKEGIFAGVSSGAVVNVAIRTAHRMEKGN
                     IVVILCDGGWKYVSLGVWHKEFPALGENMYSHLWW"
     gene            complement(1649..1894)
                     /locus_tag="VN444_03895"
     CDS             complement(1649..1894)
                     /locus_tag="VN444_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWR20986.1"
                     /translation="MNGVPEQIAESLDLKGEVCPYTFVKTKLALDELQSGQALRIIVD
                     NPGSAENVPRSLRSEGHIVADATKLNDTDWAITVIKA"
     gene            complement(1891..2145)
                     /locus_tag="VN444_03900"
     CDS             complement(1891..2145)
                     /locus_tag="VN444_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWR20987.1"
                     /translation="MTKYSLDVTGEICPYPLMLTKQKMGKLTAGDQLVVIVDYAKSVE
                     DIPRWAKEEGYAVLAISEVGRTQWEIVLEKASPALLEEGK"
     gene            complement(2190..>2404)
                     /locus_tag="VN444_03905"
     CDS             complement(2190..>2404)
                     /locus_tag="VN444_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HWR20988.1"
                     /translation="LQALGGGLLMGIGAGIAGGCNIGHGLTGVPLFGLSSVTATLSII
                     LGVWVGAYLLFGSVAFERGTRNAQRA"
BASE COUNT          510 a          758 c          567 g          569 t
ORIGIN      
        1 tgacggcggc cgcaaagagg gtaatgccta tgagcgtgat ctgcccgatg tcaaggctca
       61 ttatgcccct tacttccatt catcgcacgg ccatccatcc cggcttcgga ggctctgcac
      121 actacttctc actccctcaa gaaaccctgg caaagaagct agccgccccg ctcaaagacg
      181 gcagatgact cttctgtaaa ctgttttacg atagagtccc acctgaatac cttgaagtac
      241 gaaactcttc catttttcac tgacactaca agataacgta actgaggcca caatggttca
      301 ccatcgaaca gcgccagatc tcgatcggtc tcggagaagt aggcttcatg ttcggggtgc
      361 gaatgataga tgaggacgaa ttcgaggcct ttggcatcag cttcgctctg aattttcagg
      421 aggtccttcg gatccatgat gtaggcagtc ttcgcatctc tcggataacg aatcggatcg
      481 tcccgctgat actgattcgc cagattcgcg catctgcgga caatgtttgt attcggatcg
      541 cctggcttcc ctatgacgac cccgcaggcc tcatcaggat atgcctcctc ggcgtgagcg
      601 aagatttcgt tcagctccgg ttgcgtaagg accatcagtt cgacagggct caccacaggt
      661 ctaccaccaa aggtgactgt acatgttctc gcccagcgcg ggaaactcct tgtgccagac
      721 acccagactc acatacttcc acccgccatc gcagaggatt accacgatgt tgcccttctc
      781 cattcgatgc gccgtccgga tcgccacatt aacgacagct ccagaggaca cgcccgcaaa
      841 gatcccttcc ttgttggtca gatctttggt ggcggcaaaa gcgcagcggg aatcaaccat
      901 gattttcccg tccagaagac tggcgtcaag gatcggtggg atgaaaccct cctccaggct
      961 gcgaagcccc tggaccaagt cgccaggatt cggctccacg gcaataatct tggtcttggg
     1021 gttacactcc ttcagccgtc tccccacgcc catcagggtt ccgccggtcc caagaccggc
     1081 cacaagaaca tcaacctccg ggaggtcgtt caggatctcc accccggtcg tttcatagtg
     1141 ggccagaggg ttggccggat taccgtactg aaagggcatg tagagggttg gatcctgcgc
     1201 cacaagcttt tgagccaact caatagcgcc gttgctccct ttcgtcccat cagaatagat
     1261 caactcggcg ccgaagatct caaggagttg acgtctctcc ggggtggcgt tttccgggat
     1321 caccgccttg accttgtacc ctttcctccg cccgatcatg gccagggcga tgccggtgtt
     1381 gccgctcgtt ggttccagaa tcgttttgtc cttcgtcagt tcgccactcc gctcggcctg
     1441 ctcgatcatg tacttcgcta tccgatcctt caagctgccg gtcgggttca ctccctccag
     1501 cttggcgaag atccgaacct ctttcttagg actcatccgc ggcagttcga tcagcggcgt
     1561 gtgcccgatc gcatcaagaa tatcgactga tttcatccat gtcctctttt tcggctttct
     1621 cggctccggc ccaccttcac cttacgggtc aggcctttat taccgtgatc gcccagtcag
     1681 tgtcattcaa cttcgtcgca tccgcaacga tatgcccttc actgcgtaga ctcctgggaa
     1741 cgttctcagc ggagccaggg ttatccacga tgattctcag cgcctgaccg ctttgtagct
     1801 cgtccagcgc caatttggtc ttcacgaacg tgtaggggca tacctcccct ttcagatcga
     1861 gggactcggc tatctgctca ggtaccccat tcatttcccc tcctccaata atgcaggtga
     1921 ggctttttcc aggacgattt cccattgagt tctgccaact tccgaaatgg cgaggaccgc
     1981 atacccttcc tccttcgccc atctggggat atcttcgacc gatttcgcgt aatcgacaat
     2041 gaccaccagt tgatcaccgg ccgtaagctt ccccattttc tgcttagtca acatcaaagg
     2101 atatggacaa atctctccag tcacatcaag ggagtacttc gtcatcactg cccccatgat
     2161 cttacgatcg ccaccacgtt cggaagactc tacgcccgct gggcgtttcg cgtcccacgc
     2221 tcaaacgcaa cgctaccaaa taatagatag gctccgaccc agactccaag gataatgctg
     2281 agtgtcgccg tcacactgct cagtccaaag agtggaactc cagtcagccc atggccgatg
     2341 ttgcatccac ccgcaatgcc agcccctatg cccatcagca gaccaccacc gagcgcctgg
     2401 agaa
//
LOCUS       DATMXI010000210         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_274514, whole genome shotgun sequence.
ACCESSION   DATMXI010000210 DATMXI010000000
VERSION     DATMXI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274514"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VN444_03910"
     CDS             <1..972
                     /locus_tag="VN444_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR20989.1"
                     /translation="VQDPRFKVRGFDSNPQPPTTGASAPAVSELGVLQRELEQVKGMV
                     SGLYGRGHLPSDLPQTLGRMYWSLLSQEVEEGVARRWVVSVRDRLGEAALSDSTPISA
                     LLAEVLAREIVGLPVPKAGNGRRIVVLVGPTGVGKTTTIAKLAAGCRFREGKRVALIT
                     TDTYRIAGAQQLKNYAELIGLPYCVAPLPADLKRALASDRHADVIFIDTVGRSARRQD
                     QLEELATYSGSDDGYEVHLVLSATTKRADLLQAVEAYRPLGFSSIIATKLDETATVGP
                     VCEAALTAAVPISYLTTGQEVPDDIEEAQPLRLAWRLVGESEGGKER"
     gene            969..1832
                     /locus_tag="VN444_03915"
     CDS             969..1832
                     /locus_tag="VN444_03915"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HWR20990.1"
                     /translation="MRQMQDQASKLRGSAIATERGLRTIAITSGKGGVGKTSLAVNLG
                     LLLAGEGHRVALLDGDMGLANVDVLLGLAPKFTLRHVVEGQKELQDIMLEGPNGLYVI
                     PASRGVEAMANLAPAEQARLLGRLGQLEGLIDILLLDTAAGISPNVLSLILAADEAIV
                     VTVPEPTAITDAYAVIKVLSRHRADPRAGILMNMVEGASQAEELFEQLQRVIRHFLKV
                     EVGFVGHVLRDDCVGRAVCEQKPFSVCFPYAKASRSLVDLAKTLTTSSCRAPQPEPFW
                     DRIVYWTSQLS"
     gene            1941..3320
                     /locus_tag="VN444_03920"
     CDS             1941..3320
                     /locus_tag="VN444_03920"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWR20991.1"
                     /translation="MEITREDWKAYRQTKDPGLRDRLITANISLVRQVAGRLSLQLPS
                     CVELDDLESAGAVGLVSAVEHYDPDQPSEFATYAQYRIRGAILDDLRAQDFAPRSLRT
                     KAREIQQTLVRLTVGLGREPSDDEVAAALEMDRETYWRTLGEIRGMVLVSLDEPGVEA
                     DGSESDRPIWELPDRAGRDPSTEAARKERVQLLGGIIEALPAQERLVLTLYYFEELTM
                     KEIGRVLEVSESRVSQIHTSAILRMRARLLQIKLHRDDLIAEESATFTLRELVLAVLL
                     SVGVLVGGCVSPAAPPTLQPVVISREGRTVVLPALVSVEDRPLVEEVARLFVLELSHA
                     GIDTRGPDWVREEARARGWDWRGGQWPQISDSILDTRQSTVEMGGVNWDALGVRRVMM
                     VNLYGVDQHWEGTVRVTRVGVEARLIALPSGALLWAGKIAPGSAGTVGWSFEHATRIA
                     VRRLVESIQ"
     gene            3334..>3542
                     /locus_tag="VN444_03925"
     CDS             3334..>3542
                     /locus_tag="VN444_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR20992.1"
                     /translation="MDSLPRINEPLPDVEAVERANDIRRQARGGPKGTPQRRPKKGEP
                     RPATVSRDRHESHAEEDGGRPLQTGS"
BASE COUNT          661 a          973 c         1253 g          655 t
ORIGIN      
        1 gttcaagacc caagattcaa ggttcggggt ttcgattcca acccccaacc cccaaccacc
       61 ggtgcgtccg cgccggccgt gtccgaactc ggagtgttgc aacgggagct tgagcaggtc
      121 aaagggatgg tgtccggtct gtacgggcga gggcacctgc cgtccgatct gccgcagacg
      181 ctcggccgga tgtactggtc gctgctctcc caggaggtgg aggagggcgt agcccgtcgg
      241 tgggtggtct ccgtacggga cagacttgga gaggccgcat tatccgattc gacgccgata
      301 tcggcgttgc tcgctgaggt gctggctcgg gagatcgtcg gcctgcccgt tcccaaggcc
      361 ggcaacgggc gacggatagt ggtgctggtt gggccgactg gcgtcggcaa gactacgacc
      421 atcgcaaagc tggcggccgg atgtcgtttc cgcgagggaa aaagagttgc gctgattacc
      481 accgacacgt atcggatcgc cggcgcccag caactgaaga actacgccga actgatcgga
      541 ctgccctatt gtgtggcgcc gcttccagcg gacctgaagc gggcgcttgc gagcgatcgg
      601 cacgccgatg tgatcttcat tgatacggtc ggacggagtg cgaggcggca ggatcaattg
      661 gaggagttag cgacctattc cggcagcgac gacggatacg aggtgcacct ggtgttgagc
      721 gcaactacca agcgggctga tctgttgcag gccgtcgagg cgtacaggcc gctgggcttc
      781 agttccatta tcgccaccaa actcgatgaa accgctaccg tggggccggt gtgtgaggcc
      841 gcactgacgg ctgctgttcc catttcatat cttacaacgg gtcaggaagt tccggacgac
      901 atcgaagagg cgcagccgtt acggctggca tggcgtctgg tcggtgagtc ggagggcggg
      961 aaggagagat gaggcagatg caggatcagg catcgaagct ccgcggcagc gcgatcgcga
     1021 ccgaacgagg gctccggacg attgcgatca ccagcggcaa aggaggcgtc ggcaagacgt
     1081 cgttggccgt gaatcttggc ctgttgctgg ccggggaagg ccaccgcgta gccctcctcg
     1141 acggcgatat gggtcttgcg aatgtagatg tgctgttggg gctggcgccg aagtttacgc
     1201 ttcgtcatgt cgtcgaagga cagaaggagc ttcaggacat tatgctcgaa ggcccgaacg
     1261 gcctgtacgt catcccggcg agtcgagggg tcgaagcgat ggccaacctc gcgcctgcgg
     1321 agcaggccag gctgctgggg cggctgggac aactggaggg attgatcgac atcctgctgc
     1381 tggataccgc ggcgggaatc tcgcccaacg tcctcagtct gatcctggcg gccgacgagg
     1441 cgatcgtcgt cacggtcccg gagcccaccg ccatcaccga tgcctacgcg gtgatcaagg
     1501 ttctgtcgcg ccatcgcgcc gatccgcgtg cgggcatcct gatgaatatg gtggaagggg
     1561 cctctcaagc cgaagagctg ttcgaacagt tgcagcgggt gatccggcac ttcctgaagg
     1621 tggaggtcgg gtttgtgggc catgtgcttc gggatgactg cgtcggacgc gcagtctgcg
     1681 aacagaagcc gttctcggtc tgcttcccct acgcgaaagc ctcgcgctcg cttgtcgatc
     1741 tggcaaagac gctcacgacg tcgagctgtc gcgcaccgca accggagccg ttctgggacc
     1801 gaatagtgta ctggacgtcg cagttatcgt aaagccgtcg gcatgcagcg atcagcaatc
     1861 agcttttccg atacgctgtt ccgttttgtc tgaagctgac gactgaacgc tgactgctga
     1921 ttgccattgt ctgaggaaaa atggagatca cgcgggaaga ctggaaagcc taccggcaga
     1981 cgaaggatcc cggtctgaga gatcggttga ttacggcgaa catctccctt gtgcgccagg
     2041 tcgccgggcg gctctcgttg caactgccgt cgtgtgtcga gttggacgat ctggagagtg
     2101 ccggtgcggt aggtctggtg agcgccgttg agcactatga cccggatcag ccgtcggagt
     2161 tcgcgaccta tgcccaatac cggatccgtg gcgccattct ggacgatctc agagcgcagg
     2221 attttgcgcc acgctcgctc cgaacgaagg cgcgggagat ccagcagacg ctggtccggc
     2281 tgacggttgg cctgggacgg gagcccagtg atgacgaggt cgctgcggcg ctcgaaatgg
     2341 atcgggagac ctactggcgc acgcttggcg agatcagggg gatggtgttg gtgtcgctcg
     2401 atgagcccgg cgttgaggcc gacggctcgg agagcgatcg gcccatatgg gagctgccgg
     2461 accgggcggg tcgagatccg tccactgaag cggcccgtaa ggagcgggtg caactcctcg
     2521 gcggaattat tgaggcgttg ccggctcagg agcggttggt gctcaccttg tactattttg
     2581 aggaactgac catgaaagag atcggccggg tgctggaggt ctcggaatca cgcgtctcac
     2641 agatccatac ctcggccatt ctccgaatgc gggccagact tcttcagatc aagctccacc
     2701 gggacgacct catcgcagaa gagagcgcga ccttcaccct tcgcgagctg gtgttggccg
     2761 tcctcttgag cgtgggtgta ctggtcgggg gatgcgtatc gccggccgct ccgcccacac
     2821 tccagccggt tgtcatctcc agggagggtc ggaccgtagt cctgccggcg cttgtgtccg
     2881 ttgaggatcg accgttggtc gaggaggtgg ccaggctctt tgtccttgag ttgtcgcacg
     2941 ccgggatcga caccagggga cccgattggg tgcgggagga ggctcgagcg cgaggctggg
     3001 attggcgcgg cggccagtgg ccacagatca gcgactcgat actcgatact cgacagtcga
     3061 cagtcgaaat gggaggggtc aattgggacg cgcttggggt acggcgggtc atgatggtca
     3121 atctatatgg cgtggatcag cattgggagg gaacggtcag ggtgacgaga gtcggggtag
     3181 aggcgaggct catcgccctg ccgtccgggg cgctgctgtg ggccgggaag atcgcgccgg
     3241 ggagcgccgg gacggtcgga tggtcatttg agcacgccac ccgcatcgcg gtacggaggc
     3301 tggtggagag catccagtga aggggtacgg gtgatggaca gtctgccgag gatcaatgag
     3361 ccgttgccgg atgtcgaggc ggtagagcgg gccaacgaca tacgccggca ggcgagaggt
     3421 gggccaaaag ggacgccgca gcgcagaccg aagaaaggcg agccaaggcc ggcgacggtg
     3481 tcacgcgaca ggcatgagtc tcacgcggag gaggatggcg gtcggccgct gcaaaccggt
     3541 tc
//
LOCUS       DATMXI010000211         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_286179, whole genome shotgun sequence.
ACCESSION   DATMXI010000211 DATMXI010000000
VERSION     DATMXI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286179"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VN444_03930"
     CDS             <1..150
                     /locus_tag="VN444_03930"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWR20993.1"
                     /translation="IMHCLPAHRGEEITDEVLDGPHSIVYDEAENRLHVQKAILAKLL
                     GHHTP"
     gene            183..1475
                     /locus_tag="VN444_03935"
     CDS             183..1475
                     /locus_tag="VN444_03935"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWR20994.1"
                     /translation="MQAKRVKKIVLAYSGGLDTSVILRWLIETYQAEVIAFCADLGQG
                     EELDEVREKALKTGASKVYIKDLREEFVRDFVFPCVKANAVYEGRYLLGTSMARPLIA
                     KHQMAVASKEGADAVAHGSTGKGNDQVRFELSYYAIDPHIQVIAPWREWRLKSRSDLI
                     AYAKRHDIPVTTTKARPYSTDRNLFHISFEGGVLEDPWQEPPEEMFVLSVSPEKAPDR
                     ATYVEVEFQDGQPVALDGKRLSPARLLQRLNTIGGEHGIGRVDVIENRYVGMKSRGVY
                     ETPGGTILHAAHRAVESLTMDREVMHLRDSLIPRFSELVYYGYWFAPEMELLMRTIEA
                     SQQGVTGTARVKLYKGNCEVVGRKSAVSLYDPAFATFEADQVYRQSDAEGFIRLNALR
                     LRIRASKKQCKNPPTPPLQKGGTGGFRAMGTKGKRSGR"
     gene            1472..>2759
                     /gene="argH"
                     /locus_tag="VN444_03940"
     CDS             1472..>2759
                     /gene="argH"
                     /locus_tag="VN444_03940"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWR20995.1"
                     /translation="MTVKRKAPKPWGGRFAQATDPGVEAYTASIQFDRRLYKYDIAGS
                     VAHARMLAKCGLLSKAEADKIVAGLEEIAGEIERGEFRFDPSLEDIHMAIEVRLIEKV
                     GEAGAKLHTGRSRNDQVALDLRLYQREEIGEVCRLIVGLERAMIVQAEAHLDLIMPGY
                     THMQRAQPVLLAHHLMAYVEMLERDRARLHDALRRVDVLPLGSGALAGVGLPIDRRFV
                     ARELRFRALSANSLDAVSDRDFVIEPLFVFALLMTHLSRLAEEIVLWASAEFGFIELP
                     DAFATGSSMMPQKKNPDVAELARGKTGRVFGALVALLTIVKGLPLSYNRDLQEDKEPL
                     FDSVESVKATLLVMASLVSRLTFCADRMRQAAEDGFLNATDLADYLVRKGMPFRQAHE
                     VVGLLVRRALAMRCRLEELSLKELQVASPLFEKDVFT"
BASE COUNT          539 a          779 c          918 g          523 t
ORIGIN      
        1 atcatgcact gcctgccggc gcatcgtggc gaagagatta ccgatgaggt ccttgatggc
       61 ccacactcga ttgtgtatga cgaggctgaa aatcgactgc atgtgcagaa ggccatcctg
      121 gcgaagctgt tgggacatca cacaccgtaa tggggatgag aaaagctcct atgaggtcac
      181 aaatccaggc gaagcgagtg aagaagatcg tactggccta ctccggcggg ctcgacacgt
      241 cggtgatcct gcgctggctc atcgagacct accaggcaga ggtcattgcc ttctgcgccg
      301 atctggggca gggggaggag ctggatgagg ttcgcgagaa agcgctcaag acgggcgcca
      361 gcaaggtcta catcaaagat ttgagggaag agtttgtccg ggacttcgtc tttccctgtg
      421 tgaaggcgaa tgccgtctat gagggacgct acctgctcgg tacctcgatg gcgaggccgc
      481 tgattgcgaa gcaccagatg gcggtggcga gcaaggaggg cgcggacgcg gtggcgcacg
      541 gctcgaccgg caaagggaac gatcaggtcc gctttgagct ttcctactat gccatcgacc
      601 cgcacatcca ggtcatcgcg ccatggcgcg aatggcgtct caagtcgcgg tctgatctta
      661 ttgcctacgc gaagaggcac gacatcccgg tgacgaccac gaaggcgcgg ccgtacagca
      721 ctgaccgtaa cctgtttcac atcagcttcg agggcggggt attggaggac ccttggcagg
      781 agccaccgga agagatgttt gtcttgagcg tctcgccgga gaaggcgccg gatcgcgcga
      841 cctatgtcga ggtggagttc caggatggtc agcctgtcgc actggatggt aagcgcctct
      901 cgccagccag gctgcttcag cgcttgaaca cgatcggcgg agaacacggg atcggccgcg
      961 tcgatgtgat cgaaaaccgc tacgttggga tgaagtcgag aggggtgtac gagacgcctg
     1021 ggggaaccat cctgcatgcc gcccaccgcg ccgtggaatc gctcacgatg gatcgcgagg
     1081 tgatgcacct ccgcgactcc ctcatccccc gcttctcgga gctggtctac tatggctatt
     1141 ggttcgcccc ggagatggag ctgctgatgc ggacgatcga ggcgtcgcag cagggggtga
     1201 ccggtacggc gcgggtcaag ctctacaaag gcaactgcga ggtcgtgggg cgcaagtcgg
     1261 ccgtctcgct gtacgatccg gccttcgcga catttgaggc ggaccaggtg taccgccagt
     1321 cggacgccga ggggttcatc cgcctgaacg cgttacggct gaggattcgg gcgtcaaaaa
     1381 aacagtgcaa aaatcccccc accccccctt tacaaaaggg gggtacggga ggatttaggg
     1441 cgatgggtac gaaagggaag cgctcaggcc gatgacggtg aaacgaaaag cccccaagcc
     1501 ctggggcggg cggttcgcgc aggcgaccga tccgggagtg gaagcgtata ccgcctccat
     1561 ccagtttgac cgtcgcctct acaagtacga cattgccggc agtgtcgcgc acgcgcggat
     1621 gctggcgaag tgcggcctgt tgagtaaggc cgaggcggat aagatcgtgg ctgggcttga
     1681 agagatcgcg ggcgagatcg agcgcggaga gtttcgtttc gatccctcgt tggaagacat
     1741 ccacatggcg atcgaggtcc ggctgattga aaaagtggga gaggccggcg cgaagctcca
     1801 caccggccgg agccggaatg accaggttgc actcgatctt cgcctctatc agcgggagga
     1861 gatcggagag gtttgccgcc tgatcgtagg gcttgagcgg gccatgatcg tgcaggccga
     1921 agcccacctt gacctgatca tgcccggcta cacccacatg cagcgggctc agccggttct
     1981 cctggcgcac cacctgatgg cctatgtcga gatgctggag cgggatcgcg ccaggcttca
     2041 cgatgcgctt cgcagggtag atgtgctccc gctcggctca ggcgccctgg ctggggtcgg
     2101 cctgccgatc gatcgccgct tcgtcgccag ggagcttcgg tttcgggcgc tgtcggccaa
     2161 ctcgctggat gcggtgtcgg accgtgactt tgtcatcgag ccgctgttcg tcttcgcgct
     2221 cctgatgacg cacctgtccc gcctggcaga agagatcgtc ctgtgggcat cggcagagtt
     2281 tggattcatc gagctgcccg atgccttcgc taccggctcc agtatgatgc cccagaaaaa
     2341 gaacccggat gtggcggagc tggcacgagg gaagaccggc cgcgtgttcg gcgccctggt
     2401 ggcgctgctt accattgtca agggactccc gcttagctac aaccgcgatc tgcaagagga
     2461 caaggaaccc ctcttcgaca gcgtggagag cgttaaggct accctcctcg ttatggcgtc
     2521 gctggtgagc aggctcacgt tctgtgcgga tcgtatgcgg caggcggcag aggacggatt
     2581 cttaaatgcc accgatctgg ccgactacct ggtgcgcaag ggaatgccat tccggcaagc
     2641 ccacgaggtg gtgggccttc tcgttcgacg cgcgcttgcc atgcggtgtc ggctcgagga
     2701 gctttcgctg aaagagttgc aggttgcttc acccctgttc gagaaggacg tctttacgt
//
LOCUS       DATMXI010000212         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_60476, whole genome shotgun sequence.
ACCESSION   DATMXI010000212 DATMXI010000000
VERSION     DATMXI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60476"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..256)
                     /locus_tag="VN444_03945"
     tRNA            complement(179..256)
                     /locus_tag="VN444_03945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Arg,seq:gcg)
     gene            complement(521..1519)
                     /locus_tag="VN444_03950"
     CDS             complement(521..1519)
                     /locus_tag="VN444_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWR20996.1"
                     /translation="MGELRRDPVKNRWVIIDPERPDREAALKVEAQPPPPLSGPCPLC
                     PGNELMTPPEIVAFGEPLRQRNQPGWWVRVTPDLQPLCRIEGDFDRRPEGPFDVMNAV
                     GAHEIVVESPEHHLTWAELPEQQLERVLRAYRLRSLDLRLDGRFRSLIVVKNHADAAS
                     ILQHPHSHVLAFPFVPYGIEEELRGCREFYARKERCAFCDIMLHERVSRVRRVEETDH
                     FVVLAPFASRFPFETWVLPLRHASDFASIGDGELVDLAGLMKRMMRMLGKVLGNQSCT
                     IVLHTAPFDEPHTDDYHWHLEILPKTATVEGFGWGARLFVNPVTPEEAAARLRQAI"
     gene            complement(1623..2510)
                     /locus_tag="VN444_03955"
     CDS             complement(1623..2510)
                     /locus_tag="VN444_03955"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWR20997.1"
                     /translation="MKRMVMAIVAAVTLLTAIPGLTMAAGYGNPQFLVDTNWLALHLN
                     DRDLRIIDMRNSPEEYAAGHIPGAVHLAVNQVRLTVKESGFAMPPDYEIEERLGQLGI
                     TKETMVVAYDDQGGLNAARLFFTLEYAGHKKVAVLNGGITKWTAEKRSLSNTLPQVSK
                     TVYQVHAETQRVAPAGWIVTNLGKPNLALVDARSPAEFRGEDLRAKRGGHIPGAVNIE
                     WTQTLAGDKTFKPADELMALYSQAGVTKDKTIVSYCQTMHRGAVTYFTLRLLGYPDVR
                     GYDRSWSEWGNDPALPVQW"
     gene            complement(2678..3151)
                     /locus_tag="VN444_03960"
     CDS             complement(2678..3151)
                     /locus_tag="VN444_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR20998.1"
                     /translation="MIPRYRKLLVTTDFSALGNSAIPHAYAILAERGGTVILCHVTEV
                     HGPPNPLYAHYSPWSGLSGQERAELRQTLLSSLESLRPEEASVEGMVATEVRVVETPL
                     LVHEAICQEATELEVDLIVMASHGHSGIARLFLGSVTEHVLRSADRPVLIVRSRG"
BASE COUNT          738 a         1108 c         1020 g          720 t
ORIGIN      
        1 tggagtaccc tggttccagt gggatggcca tgcggtcctc ccgctccgag aggcaaaggt
       61 tgtgacgatc cttactccaa acctgggagg cggtcaagcc tttttggcgg cgactcagga
      121 atcgaagcgt agcgcctcgg caggacggta agagaaaata tgacgagggg gattttagtg
      181 gtgcgcccgg gcagatttga actgccggcc ttttgctccg caggcaaacg ctctatccgg
      241 gctgagctac gggcgcaaag caactgtgga aggaacgggc cgactgtagc atgaccacca
      301 tgaacggtca agccgtaagc agccgtctaa cagcaggcgc gtaaccggcc tgtggcagcc
      361 tctgcttact gcgggtccgt gttgacgcgc gggttaggct ggtccacctc taagctgctt
      421 atggtagatc ttggatgatg atactaccac ccaccacgac cagggcgtca ccctggtcca
      481 gggtgaactt ctggggcccc agtgggcaca tcgggtcacc ctatatcgcc tgtcgtaacc
      541 gggcggctgc ctcctcaggt gtaaccgggt tcacaaagag ccgcgcaccc cacccgaacc
      601 cctccacagt cgcagtcttc ggcagtattt cgagatgcca gtgatagtca tccgtatgtg
      661 gttcgtcaaa cggggcggta tggaggacta tcgtacagga ttggttgcca aggaccttcc
      721 cgagcatccg catcatccgc ttcatgagac cggcaagatc gaccagctcc ccatcgccga
      781 tactcgcgaa gtcagaggcg tgacgcagtg ggagcaccca cgtctcgaaa ggaaagcgag
      841 aggcaaaggg cgccagcacc acgaagtggt cagtctcctc cacccttctg acgcgggaga
      901 ctcgctcgtg gagcatgatg tcgcaaaagg cgcagcgctc ttttctcgca tagaactccc
      961 gacagccgcg tagttcctct tcaatcccgt acgggacgaa cgggaaagcc agcacgtgag
     1021 aatgggggtg ctggaggatg ctcgctgcgt ctgcatggtt cttcaccacg atcagcgatc
     1081 ggaaccgccc atccaggcgg agatccaggc tccgcagtct gtaggcacgc aggacccgct
     1141 ccaactgttg ctccgggagt tctgcccacg ttaaatgatg ctctggactc tccacgacga
     1201 tctcatgggc ccccacggcg ttcatgacgt cgaacggccc ctccggcctt cgatcgaaat
     1261 caccctcaat ccgacaaaga ggctgcaggt ccggggtgac tcgaacccac cagcccggct
     1321 gattccgctg acgcaaaggt tcgccaaacg cgacaatctc aggcggagtc atcagctcat
     1381 tgcctggaca gagcgggcat ggacccgaaa gcggtggagg tggctgtgcc tccaccttca
     1441 aggcagcctc tcgatctggc cgctccgggt cgatgatcac ccaacgattt ttcaccggat
     1501 cgcgccgcaa ctcacccatg tctctcctta cgtcacaaca aaaggtggca gcctacctca
     1561 gtgaagatct gccgcgcgtc cagccgcccg gccgcagaac gtctttgacc ttcaccgcca
     1621 actcaccact gcaccggcag cgcagggtcg ttaccccact cgctccagga gcgatcgtac
     1681 ccccgaacgt ccggataccc caggagccgg agtgtgaagt aggtgacggc tccgcgatgc
     1741 atggtctgac agtagctcac gatcgtcttg tccttcgtca cccctgcctg gctatagagg
     1801 gccatgagtt catcggccgg cttgaatgtc ttgtctccgg ccagggtttg cgtccactca
     1861 atgttcacgg cccctgggat gtggcctccc cgcttggccc gcagatcctc accgcgaaac
     1921 tccgcaggcg accgggcgtc taccagggcg agattcggct ttcccaggtt tgtcacgatc
     1981 cagcccgccg gcgcgacgcg ctgcgtctca gcatggactt ggtagaccgt cttactcacc
     2041 tgtggcagcg tattcgagag ggaccgcttt tcggctgtcc acttcgtgat acctccgttc
     2101 aacaccgcaa ccttcttgtg gccggcatat tcgagggtga agaacaggcg tgcggcgtta
     2161 aggccgccct ggtcgtcgta cgcaactacc atcgtctctt tggtaatccc aagctgtccc
     2221 aaccgctcct cgatttcata atcgggaggc atggcgaaac ctgactcttt cacggtcaga
     2281 cggacctgat tgacggcaag gtggaccgca cccggaatgt gtccggccgc gtactcctcc
     2341 gggctgttgc gcatgtcgat gatccgcaga tcccggtcat tgaggtgcaa ggccagccag
     2401 ttagtatcca cgagaaactg tgggttgcca tagccggcag ccatagtcaa ccctgggatc
     2461 gctgtgagca gcgtcactgc tgccactatc gccatcacca ttcgcttcat ctcttccctc
     2521 ctgtcccaga taccgcggaa aagcaatccg attcgccacc tctgtttgcc aagcggctcg
     2581 ctctcccaca cattatagat ttccaccaag gcttgacgca agcgcgatgt cagcgtagcg
     2641 cgcgcgatag cgctgcacct tcaccggcat aagaagctca ccctcggcta cggacaatga
     2701 ggacggggcg atcggctgac cgtaacacat gctctgtcac tgagcctaag aacaggcgag
     2761 ctatcccgga atgaccatgg gatgccatca cgatgaggtc cacctccagt tccgtggcct
     2821 cctggcagat cgcctcgtgg accagcaaag gggtctctac cacccgcacc tccgttgcta
     2881 ccatcccctc tacgctggcc tcctcaggcc taagtgattc aagcgagctg agcagtgtct
     2941 gccggagttc cgctcgctcc tgccccgaga gtccgctcca tggcgagtag tgggcataga
     3001 gcggatttgg cggcccgtgc acctcagtaa cgtggcagag gataacggtc ccgccgcgtt
     3061 ctgccaggat agcataggca tgggggatgg cactattgcc aagggctgag aaatcggtgg
     3121 tcaccagcag tttccgatac ctcggtatca tggtatgttg gctccttccg tcaagcaggt
     3181 tctactgcca tccgctcact gacattgggc agatcgtcct gacgaccctc tagcgtacct
     3241 gtcaggttta acatctggga gagcttcctc agtataacct tcatcgccac ctttgtgaag
     3301 gagacccggt cactgggctg ccggaacaca gcatcatccc gcacttgatt gatgggtctt
     3361 tcagagtcgc agggaaagat cccgtccggc actatctgaa ggcaggctga tcggccaagt
     3421 ggatgaggag tgggccttga aaataagtgg ccatcgcact atactagtag tgagggagcc
     3481 caagtgatcg ggcaatggct gaagaaatgc gagcgagcac gaagtaatat gtgcttgcag
     3541 agcaagaggg cttagcctgg gaccagaacc cgttcggaag gggctt
//
LOCUS       DATMXI010000213         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_124637, whole genome shotgun sequence.
ACCESSION   DATMXI010000213 DATMXI010000000
VERSION     DATMXI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124637"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /gene="rpsD"
                     /locus_tag="VN444_03965"
     CDS             <1..433
                     /gene="rpsD"
                     /locus_tag="VN444_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWR20999.1"
                     /translation="IYGVLETQFRKYYRLAERQTGITGENLVKILEQRLDSVVHRLGF
                     ATSRAQARILITHGHILVHGGKTDIASYRVRPGETIEVRPKSREIAAIKTAFEVAKRR
                     TLPSWLELDPTNMKGVVRSIPSREEIAVPVEEQLIVALYSK"
     gene            455..1483
                     /locus_tag="VN444_03970"
     CDS             455..1483
                     /locus_tag="VN444_03970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWR21000.1"
                     /translation="MIQKFKGLQKPKRLECELESLTSTYGKFITEPFESGFGLTIGNA
                     LRRILLSAIEGAAVTAIRITGALHEFTTIPGVKEDVSDIILNLKGLRIKLHVDHPKTL
                     HLKVFSEGEVRAAHITPDPDVEILNPDLHIATLALDSSLDLELEVCHGRGYVPAERNK
                     REGQPVDVIAVDSMFSPIRKVAFLVEATRVGQITDYDKLTLEVWTDGSILPRDAIAYA
                     AKTLKDHVSIFTNFEEEPEREGVVVDEAKKQLLDNLSRSVDELELSVRSANCLKHSDI
                     RNIYELVVKSEAEMLKTKNFGRKSLNEIKEILLGMGLTLGMKLEGLSVGESFGKRGDK
                     TSKVLTGA"
     gene            1524..2015
                     /gene="rplQ"
                     /locus_tag="VN444_03975"
     CDS             1524..2015
                     /gene="rplQ"
                     /locus_tag="VN444_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWR21001.1"
                     /translation="MRHRNAGRKLGRTTAHREMLLRNLVTSLFQYEKIVTTEAKAKEL
                     RKLADKIVTLAKRGDLHARRQAAEVVQDETVLKKLFDSIGSRYKDRNGGYTRMTKLNY
                     RMGDGAPLAAIELAGVGADAEPAAKPASRRGRREDKGKVPKPRQAKPRVASPPAIESA
                     VSP"
     gene            2182..4266
                     /gene="fusA"
                     /locus_tag="VN444_03980"
     CDS             2182..4266
                     /gene="fusA"
                     /locus_tag="VN444_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWR21002.1"
                     /translation="MSGIGIARIRNVAIVAHGGAGKTTLTEAMLFDAGATSRLGKVED
                     GTTVTDFDEDEIKRKMSVSSAFAFCEWKGYKLNLIDTPGASIFLTDTRNCMRVVDGAV
                     VVVSGVSGVKVQTEKVWVYAEAEGLARAIIINKMDREQADFFRALEDIRQHLCPVATP
                     IQIPIGTQASFMGVVDLLRMKALIYQGERTGKFSEAEISPDLRPKAEQMQTALVEAVA
                     DSDDRLLEKYLETGTLSCDELKAGLQRAVIRGKLVPVLCGSALKNIGVQPLLDLLTEV
                     FPSPTDREAIVGIDPRNGEPLIRESREDAPLSALVFKTLVDPYAGKMTLFRVYSGMLS
                     SDSSVYNSTKGCKERIGQVVLLRGKHQVPVPAIGAGDLGAVVKLKETGTGDTLCDERS
                     PIRLEPVRIPSPIIEYAIAPKTRGDDEKMSLGLHRLQEEDPSLQVRRDPQTKEIILAG
                     MGKAHLEMAVERLKRRFGLEVQMKTPRVPYKETIHGRTEVQGKHKKQTGGHGQYGDCW
                     IRLESLPRGAGYEFVNHVVGGAIPKQYIPAVERGIMEAMEEGSLVGYPVVDVKVTLYD
                     GSYHAVDSSEMAFRVAGSLAFKRGVLQAHPTLLEPIMTVEVTAPDECMGDVIGDLNSR
                     RGHVMGVEVKGKGQTIKAQVPMAEVLEYATQLKAITGDRGDYTVEFSHYDEVPQHLKE
                     RVIAELKKVKGE"
     gene            4382..4843
                     /gene="rimP"
                     /locus_tag="VN444_03985"
     CDS             4382..4843
                     /gene="rimP"
                     /locus_tag="VN444_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWR21003.1"
                     /translation="MTGEVVERVKLIAIPLFTELGLELVDVEFRREASGWVLRLYLDK
                     PGGVVVGDCQRVSEELSDLLDIEDLIAHPYTLEVSSPGLNRPLRLESDFLRFVGQRAR
                     ISTSSAVGGQRRFLGVLRGYEDGQVLLEREDGTIALIPYALIVKSRLDPVL"
     gene            4940..>5606
                     /locus_tag="VN444_03990"
     CDS             4940..>5606
                     /locus_tag="VN444_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HWR21004.1"
                     /translation="MGIDLLQVIEQVGREKEIDSAVLIEAVSAAILSASRKTIGSALD
                     LRVEFNQRARCFTLYAVRKVVEEVLNPHVEISIDEARSINPGAQLGDEIKSEMKAKEF
                     GRIAAQTAKQVIIQRVREAERESVFQAFKARVGEMVGGVVQRVVKGNVIVNLGKAEAI
                     LPPREQLPREDHRTGDRVRAYVLDVKKLPRGSQIVLSRTHPGLLAKLLEIEVPEIYEG
                     IVEI"
BASE COUNT         1334 a         1354 c         1735 g         1183 t
ORIGIN      
        1 aatctacggg gtgctggaaa cacagttccg aaaatactat cgtctggccg agcgacagac
       61 agggatcaca ggcgaaaacc ttgtcaagat tctggagcaa cggctcgata gcgttgtcca
      121 tcgtctcgga tttgccacat cccgcgcaca ggcccggata ttgattaccc acggtcatat
      181 cctcgtccat ggaggaaaga ccgacattgc gtcctaccgg gttcgtccgg gcgagaccat
      241 cgaggtgcgg ccaaagagtc gcgaaatagc ggcaattaag acggccttcg aagtggcaaa
      301 gcggcgaacg ttgccgagct ggctagagct tgatccgacc aacatgaagg gggttgtccg
      361 atcgatccca tcccgagagg agatcgctgt ccccgtggag gagcagttga ttgttgcgct
      421 gtactccaaa taatggtggg cgtgggaggt tttaatgatt cagaagttta agggtctcca
      481 gaagcctaag cggctggagt gcgagctaga atctctgacc agcacatacg gcaagtttat
      541 cacagagcct tttgaaagtg ggtttggttt gaccatcggt aatgcgcttc gacgcatcct
      601 cctctccgcg attgagggag ctgcggtaac agcgatccga atcaccgggg cgctgcatga
      661 attcacaacc atccctggag tgaaagaaga tgtgagcgat atcatcctca acctcaaggg
      721 gttgcggatc aagctgcacg ttgaccaccc caagaccctg cacctcaagg tgttttcaga
      781 gggagaggtt cgagccgcgc atatcactcc cgatcctgat gtcgagatcc taaaccctga
      841 tttgcatatt gcgaccctgg cgttggatag cagtctcgat ttggagttgg aggtgtgcca
      901 tgggcgaggg tatgtccctg cagagcgaaa taagcgagag ggacaacctg ttgatgttat
      961 cgccgtagac tctatgttct ccccgattcg gaaggttgcc ttcctcgttg aagctacccg
     1021 tgtcggccag attaccgact acgataagct taccctggag gtctggactg acggcagcat
     1081 cctgcctcgg gacgccatcg cgtatgcggc aaagaccctc aaggatcatg tgagcatatt
     1141 taccaacttt gaggaagagc cagagaggga gggggttgtc gtcgacgagg caaagaaaca
     1201 gctgctggac aacctgagtc gaagtgtcga cgagttggag ctttcggtgc ggtcggctaa
     1261 ttgtctgaag cactctgaca ttagaaatat ctacgagttg gtcgtcaaga gtgaggccga
     1321 gatgttgaag acgaagaatt tcggtcggaa gtctctgaat gagatcaagg agatccttct
     1381 cggtatgggg ttgaccttag gaatgaaact ggaaggtctc tctgttggcg aatccttcgg
     1441 aaaacgaggc gataagacct cgaaggtctt aacgggggcc taagcctggc aggattttag
     1501 tcggccaggc aggagtatct gctatgcggc ataggaacgc tgggaggaag ctcggccgga
     1561 caacggctca tcgagagatg ctcctccgta atctggtaac gtcactcttc cagtacgaga
     1621 agatcgtcac aaccgaggcg aaggccaaag agctacgcaa gctggcagac aagattgtta
     1681 cgctagccaa gcgtggggat ttgcatgcgc ggcgacaggc agccgaggtt gttcaggacg
     1741 agactgtctt aaaaaagctg tttgactcaa tcggcagccg ctataaggat cgaaacgggg
     1801 gctacacgcg gatgacgaag ctgaattaca gaatggggga cggcgccccg cttgcggcca
     1861 tcgaactggc aggggtcggc gccgatgccg agcctgccgc aaagccagcc agccgccgtg
     1921 gtcggcgtga ggacaaagga aaagtaccga aaccaaggca ggccaaaccc agggtggctt
     1981 ctccacccgc catcgaaagt gcggtcagtc cttaaggata atgaggtgac ttacgcacag
     2041 gatgcccttt ccgactcgtt cgggaagggc atcgttgctt tcatgaggag atgatttccc
     2101 ctttacaact gagcggccgc tgtagtccga aaagaacctc tggactgttg agtggctgcg
     2161 cgagatatgg gggtaagggt gatgagcggg atagggattg ccaggatccg caacgtcgcg
     2221 atcgtagccc acggaggcgc aggtaagacg acgctgaccg aggcgatgct tttcgatgcc
     2281 ggtgccactt ctcgactcgg gaaggtcgaa gatgggacga ccgtcaccga cttcgacgag
     2341 gacgagatca agcggaagat gagcgtcagc tcagcctttg ccttctgtga gtggaaaggg
     2401 tacaagctta acctgattga tacccctgga gcgtccatat tcttgactga tacccgaaac
     2461 tgtatgaggg tcgttgacgg cgcagtcgtg gtggtgagtg gcgtctccgg cgtgaaggta
     2521 cagacagaga aggtatgggt gtacgctgaa gcagaaggcc ttgcgagagc gattattatc
     2581 aataagatgg atcgggaaca ggccgacttt tttcgagccc tggaagacat tcgtcagcac
     2641 ctatgcccgg tcgcgacacc catccagatt ccaataggaa cgcaagcgtc ctttatgggt
     2701 gtggtcgatc tgctgcggat gaaagccctg atctaccagg gcgagaggac ggggaagttc
     2761 tccgaagcgg agatttcacc ggacctgaga ccgaaggcag agcagatgca aacggcgctg
     2821 gtggaggcgg ttgcggacag tgatgatagg ctgttggaga agtatctgga aacagggacg
     2881 ctttcatgcg atgagctgaa ggccgggctg cagcgagcgg ttattagggg aaagcttgtt
     2941 ccagttctct gcggttcggc cctcaagaat atcggcgtac agcctttgct ggatctcctg
     3001 acagaggtct ttccatcccc gacagatcgt gaagccatcg tgggaatcga tccgaggaac
     3061 ggggagccgc ttattcgaga aagcagagag gatgccccat tgtccgctct ggtgtttaaa
     3121 acactcgtcg atccctacgc tggcaaaatg acccttttcc gggtttactc cggcatgctc
     3181 tcttcagatt ccagcgtcta caatagcacc aaagggtgta aggaaaggat cggccaagtg
     3241 gtgctgctcc gaggtaaaca tcaggttccg gttccggcaa tcggggcagg agatctgggg
     3301 gctgtggtga agctgaagga gacaggtaca ggtgacaccc tctgcgatga gcgaagtcca
     3361 atccgcctgg aaccggtgag gatcccaagc cccattatcg agtatgccat tgcgcccaag
     3421 acgagagggg atgatgaaaa aatgagcttg ggactccatc gacttcagga agaggatccc
     3481 tcccttcagg ttcgacgcga tccacagacc aaggagatca tcctggccgg gatgggcaag
     3541 gcccacttgg aaatggcggt cgagcgcctc aagcggaggt tcggactcga ggtccagatg
     3601 aagacgccac gcgtaccgta caaggaaaca attcacgggc gaacggaggt tcagggaaag
     3661 cataagaaac agacaggggg ccatggacag tacggtgatt gctggatcag acttgagtcg
     3721 ctccctcgag gcgcggggta cgaattcgtg aaccacgttg tggggggggc tatccccaag
     3781 cagtacatcc ctgcggtaga gagagggatc atggaggcga tggaggaagg gagcctggtc
     3841 gggtatcctg ttgtggatgt gaaggtcacc ctctacgatg gctcgtacca tgctgttgat
     3901 tcctcggaaa tggccttcag ggttgctggt tcattggcgt tcaaaagggg cgtcctgcag
     3961 gcacacccaa ctcttctgga gccgatcatg acggtcgagg tgacggcccc cgatgagtgt
     4021 atgggggatg tgatcggcga cctgaacagc aggcgtggac acgtgatggg ggtagaggtt
     4081 aaggggaaag ggcagactat caaggcccag gttcccatgg ctgaagtgtt ggagtatgcc
     4141 acacagctca aggctatcac cggcgaccga ggtgactata ccgtggagtt ctctcactat
     4201 gacgaggttc cccagcatct gaaagagcgc gtgattgcag aattgaaaaa ggtgaaagga
     4261 gaatagacct cccttcatcc acttctcctt gatttccctt gtacagtcca agagaaatgt
     4321 gctagcttgt taagagtggg ccatcgctgc ccgctctttg ttttataagg ggtgctgtga
     4381 tatgactggg gaggtcgtag agcgggttaa gctgatcgcc attccgctct tcacagagtt
     4441 ggggctggag ctggtagacg ttgagtttcg tagggaagca agcggatggg tcctccgcct
     4501 ctacctcgac aagcctggtg gtgtggtcgt gggggattgc cagcgagtca gcgaagagct
     4561 gagcgacctt ctcgacatag aggatcttat tgctcatccc tacacgcttg aagtctcctc
     4621 tcctggtttg aaccggccac tccgtttgga gtcggatttt cttcgcttcg taggccaacg
     4681 ggcgaggata agcacctcat cagccgttgg cggccaacgg cgatttcttg gcgtcctaag
     4741 gggttacgag gatgggcagg tattgctgga acgggaagat ggcacgattg ccctcatccc
     4801 gtatgcgctg attgtgaagt ctcgactgga cccagtcctg taaagagcag ggtatagggt
     4861 tcagggtata gaaaataaga aggcatttag ctcttttcta aaccctatac cctaaaccct
     4921 ataccctggc gtgagactaa tgggcatcga tttactgcag gttatagaac aagtcggacg
     4981 cgagaaagag atagactcgg cagttctgat cgaggcggtc agcgcggcga tcctctccgc
     5041 gtcccgcaag accattggat ccgcccttga tctgcgcgtg gagtttaatc aacgggcccg
     5101 ttgctttacg ctgtacgcag tacgaaaggt tgtcgaggag gttctgaatc cccatgtcga
     5161 gatctccatc gatgaggccc gatcgataaa ccctggggcc caacttggcg atgagatcaa
     5221 atctgagatg aaggcaaaag agttcggacg gattgctgcc caaacagcga aacaggtcat
     5281 catccagcgg gtcagagagg ccgaacggga gagtgtgttt caggctttta aggccagagt
     5341 gggtgaaatg gtgggtggcg tcgtacagag agttgtgaag ggcaacgtca tagtcaatct
     5401 cgggaaggct gaggcgattc ttccccccag agagcagctt ccccgcgaag atcacaggac
     5461 cggcgatcgg gttcgggcgt atgttctgga tgtaaagaaa ttaccaaggg gatcccagat
     5521 tgtcctttca cgaacgcacc ccggcctctt ggctaagctg ctcgagatcg aggttcccga
     5581 gatctacgag gggatcgttg agatca
//
LOCUS       DATMXI010000214         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_8249, whole genome shotgun sequence.
ACCESSION   DATMXI010000214 DATMXI010000000
VERSION     DATMXI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8249"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VN444_03995"
     CDS             <1..594
                     /locus_tag="VN444_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21005.1"
                     /translation="LSDLETRIKGEPVTQTPAMKLGAAFHKVAEGDLDLEPYFGSHSG
                     FTAASFYCADGMLFDVDSTDEALALRGPGVAEVWCSRQLLTVPYAPLLRGRSDWLSGI
                     IPYEIKTKEKDFDPTDYAASLQWQCYLHMLSTEECRYLLVRLEERVEGVWWVRDADVL
                     PLFRYVGMEEHINQWATRFMHFCEQRNLLSYLDKELL"
     gene            591..1022
                     /locus_tag="VN444_04000"
     CDS             591..1022
                     /locus_tag="VN444_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21006.1"
                     /translation="MMMPAGTYRAQAVDGHLGLTSKSNEQIVVKFVIVDGPQKDQTVS
                     WYGFFSDACFDRTIQSLRYCGWQGDDVSDMAGIDRNDVDIVVEHDEYQGKVTPKVRWI
                     NQVGGGAGMSAAQAADFAQSIKPRIAGMKSADPDAEESVPF"
     gene            1060..1284
                     /locus_tag="VN444_04005"
     CDS             1060..1284
                     /locus_tag="VN444_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21007.1"
                     /translation="MPIARPQWANEIERARANIAWGKAHPGADIRTCPREYLICLEPE
                     DFLDLIGEGTDAPEDNAELYLDNPSYGSGD"
     gene            1295..1627
                     /locus_tag="VN444_04010"
     CDS             1295..1627
                     /locus_tag="VN444_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21008.1"
                     /translation="MLRPCLMCGSLTQGVYIGRPFCLDHYLDGTLATWLQTEPTEDTM
                     TDGPSLTLRETTPPPIDLTTFAQQIEIQETPTFAGLLELMGNELYRRGMLSSGTDLKD
                     VARALREG"
     gene            1847..2044
                     /locus_tag="VN444_04015"
     CDS             1847..2044
                     /locus_tag="VN444_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21009.1"
                     /translation="MKLSILLRTQEFRGDHSADVLQAHDLLEGETVEALAGRLLQDAN
                     KWADKFNNVIEIRLIKTESTR"
     gene            2041..>2316
                     /locus_tag="VN444_04020"
     CDS             2041..>2316
                     /locus_tag="VN444_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21010.1"
                     /translation="MKDTEPVTIHSLDPPRKIGDGELFLGGHGLPMVRQFNRAPGTQY
                     DVWAFPNNWCLKGRPGPLLDAWLAIEQAMSDLAEWQRRHEGCGRTEPR"
BASE COUNT          535 a          609 c          710 g          462 t
ORIGIN      
        1 ctctctgacc tcgaaacccg gatcaagggc gagccggtca cgcagacgcc agccatgaag
       61 ctcggagccg ccttccataa ggttgccgag ggggacctgg atcttgaacc atacttcggt
      121 tctcactctg ggttcacggc agcaagtttt tactgcgctg atgggatgct ctttgacgta
      181 gatagcaccg atgaggctct cgcgcttcgt ggtccaggcg tggctgaggt ctggtgttcg
      241 cgccaactgc tcaccgtgcc ctacgcgcct ttactgaggg gccgctcgga ttggctgagc
      301 ggaatcatcc cttatgaaat taagacgaag gagaaagact tcgaccctac cgattacgcg
      361 gccagcctcc aatggcagtg ctatttgcac atgctcagca ctgaggagtg ccggtatttg
      421 ctggtgcgcc tagaggagcg agtcgaggga gtatggtggg tgagggatgc cgatgtgctc
      481 ccgctatttc gctacgttgg gatggaagaa catatcaatc aatgggctac ccgtttcatg
      541 cacttttgcg aacaacgcaa cctactcagt tatctcgata aggagcttct atgatgatgc
      601 cggccggaac gtaccgcgcc caagcggtcg atggacactt gggactgacc tcgaagagca
      661 acgagcagat tgtcgtgaag ttcgtgattg tcgatgggcc gcagaaagac cagacggttt
      721 cttggtatgg ctttttctcg gacgcctgct ttgaccgaac cattcagagc cttcgctatt
      781 gcggctggca aggagatgat gtctcggaca tggccgggat tgaccgcaat gacgtggaca
      841 tcgttgtcga acatgacgaa taccagggca aggtcacgcc gaaggtacgc tggatcaacc
      901 aagtcggggg cggggccggg atgagcgccg cccaggccgc cgacttcgcc cagtcgatca
      961 agccgcggat tgccgggatg aagagcgcgg atccggacgc ggaagagagc gtgccgtttt
     1021 gagccatcag ggccttgtct aatctgaaag gaggacatca tgccgatagc cagaccacaa
     1081 tgggcaaatg aaatcgaacg ggccagagcc aatatcgcat gggggaaggc tcaccccggt
     1141 gcagacatac gaacgtgtcc aagggaatac ctgatctgcc ttgagccgga agattttcta
     1201 gatttgatag gcgagggaac cgatgcgccg gaggacaatg cagaattgta cctcgataac
     1261 ccgtcctatg ggtcaggaga ctaaggagga cacgatgctc cgcccctgtc tgatgtgcgg
     1321 cagtctcact cagggcgtct atattgggcg gccattctgc ctcgaccact acctagacgg
     1381 caccctggcc acatggctcc aaaccgagcc gacggaggat accatgactg atggcccaag
     1441 cttgacccta cgagagacga caccaccgcc aattgacctc actaccttcg cgcaacagat
     1501 cgagattcag gagactccaa ccttcgcggg cttactagag cttatgggca atgaactgta
     1561 tcggcgcgga atgttgagca gcggaaccga cctgaaggat gtcgcaaggg cgctgaggga
     1621 ggggtgagag atgaaggctg acacagagcc agcgcaaata tggagcatcg acccgccgcg
     1681 gaagctctac gatgcggagt tatccatcgg atcccagggc cttgttgaat gcatggtcct
     1741 ttccggcgaa ctggcacctc aagggccgcc taggcgtgct gctggatgct tggcgacaga
     1801 tcgaacagat taggttagag ttggcgagat ggaaggagga taagtgatga agctctcaat
     1861 cttactccga actcaagagt ttcgcggcga tcatagcgcg gacgtactgc aagcgcacga
     1921 cctgttagag ggcgaaactg tcgaagccct cgcggggcgg ttgcttcagg atgcgaataa
     1981 gtgggcagat aagtttaaca acgtaataga gatccgccta attaagacgg agtcaacgag
     2041 atgaaggaca ctgagcccgt gactattcac tcgctcgacc cgccgcgcaa aatcggcgac
     2101 ggcgagttgt tccttggcgg tcacggcctg ccgatggtac ggcaatttaa tcgtgcaccc
     2161 ggtacacagt acgacgtgtg ggccttcccg aataattggt gtctcaaggg ccgtcccggt
     2221 cctctcctag atgcctggct cgccatcgag caggcgatga gcgatttggc ggaatggcaa
     2281 cggaggcatg agggttgcgg tagaacggag ccgcgc
//
LOCUS       DATMXI010000215         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_33579, whole genome shotgun sequence.
ACCESSION   DATMXI010000215 DATMXI010000000
VERSION     DATMXI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33579"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VN444_04025"
     CDS             <1..1264
                     /locus_tag="VN444_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWR21011.1"
                     /translation="PLSARAEYTGVNWGINLWLLAHALEFASFLMGGVNFLTTAITMR
                     APGMTFFRLPLVIWQLLTASVIFLLSVGPLIAAAVMLLMDRLLETSFFVPEGGGDPML
                     WEHLFWFFGHPEVYVILLPGLGAALEVLAVFSRKPIFGYRWIIYSTITTGVLSMIVWA
                     HHMFVSGMDPRLVMPFSISTILISVPIALIIFSMILTLWKGSIRLTTPMLWALGFIVM
                     FILGGLTGIVLGSAPANIQVHGTYFVVAHFHTVLLSVVIFGGFTGLYFWFPKMFGRMM
                     NEPLGKLHFLLTLIFFNLTFFPMHAVGLAGMPRRIANPLQYEFLTAAQPLNVQITISA
                     IGLIIAQLPFLINFAWSIVAGKKAEPNPWEAATLEWTAPSPPPHDNWGETIPTVYRGP
                     YEYSPPGVREDYLPQDRALTARSAQEKR"
     gene            1270..1836
                     /locus_tag="VN444_04030"
     CDS             1270..1836
                     /locus_tag="VN444_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWR21012.1"
                     /translation="MAAVSEQSTPALRGKIAIWWVIASEVMIFGGAVGSYIMARAASP
                     GWSAEAAHLSTSLATINTFVLLTSSMTMAMAFAANQKDDRRRVQKFLLLTILLGLAFL
                     GIKAYEYTSHIMEGAAPWSGPFWAFYYLLTGLHALHVIAGVIVNVILLLAAGKGLSTG
                     YRIEIAGLYWHFVDIVWIFLFPLLYLSY"
     gene            2113..2391
                     /locus_tag="VN444_04035"
     CDS             2113..2391
                     /locus_tag="VN444_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWR21013.1"
                     /translation="MTTERVHHNYVAIWVWLIVLMVAGVLATRLPLGKSAVNNLIFAI
                     AAIKAALVALNYMHLKSENWLIYALAIVPVLLVIAMTLVLFPDIVFHR"
     gene            2420..>2884
                     /locus_tag="VN444_04040"
     CDS             2420..>2884
                     /locus_tag="VN444_04040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR21014.1"
                     /translation="MAKGANVIIIGGGIIGVACAYALTRAHVNRVVLIERGQLGREAS
                     RASAGMLVPQGEAEGPGPFLDLCLAARDRYRTLADEIRDATGEDIEYCKWGLLYLLDP
                     TEHEAAKGRAAWQRAAGLRVEELSGREVRAMEPVLNPEIGGALFFPDEAHIRP"
BASE COUNT          522 a          850 c          808 g          704 t
ORIGIN      
        1 gccgctgagc gcgagagcag aatataccgg tgtaaactgg gggataaacc tctggcttct
       61 tgctcacgcc cttgagtttg cctccttcct gatgggtggc gtgaattttc tcacgaccgc
      121 tatcaccatg cgagcaccgg ggatgacctt tttccgtctt ccgctcgtta tctggcagct
      181 tctgaccgct tccgtcatct tcttgctctc tgtcggaccg ctgatcgcgg cagccgtgat
      241 gctgttgatg gatcggctcc tggaaaccag cttctttgta cccgaggggg gtggcgaccc
      301 catgctctgg gagcatctct tctggttctt cggtcatcca gaggtctacg tcatcctgtt
      361 gccagggctt ggggcggcac tggaggtcct ggctgttttc tcgaggaaac caatctttgg
      421 ctaccggtgg atcatctact cgaccatcac tacaggggtc ctcagcatga tcgtctgggc
      481 gcatcacatg ttcgtcagtg gcatggatcc ccggctcgtc atgccgttta gcatttccac
      541 catcctgatc tccgtgccga ttgcgctcat cattttctca atgattctta ccctctggaa
      601 aggctcgatc cgcctgacga cgccgatgct ctgggccctg ggctttatcg tgatgttcat
      661 tcttggagga ctcaccggta ttgtccttgg ttctgcgccg gccaacattc aggtccacgg
      721 gacctacttt gtcgtggccc actttcatac cgttctgtta agcgtcgtca tattcggcgg
      781 ttttaccggc ctctacttct ggtttccaaa gatgttcggt cggatgatga atgagcccct
      841 gggaaagctg cactttctgc tcacgctcat tttctttaat ctgaccttct tcccgatgca
      901 tgccgtcggc ctcgctggga tgccgcgccg tatcgccaac ccgctccagt acgagttcct
      961 gacagcggct caaccgctta acgtccagat tacgataagt gcgatcgggc tcatcatcgc
     1021 tcagcttccg ttcctgatca actttgcctg gagcatcgtg gccggtaaga aggcggagcc
     1081 taatccctgg gaggcagcaa cgctcgagtg gacagccccc tcgcctcccc ctcatgacaa
     1141 ctggggagaa acgatcccga ccgtctatcg cggcccgtat gagtacagcc ctccaggcgt
     1201 tcgtgaagat taccttccgc aggatcgcgc cctgacagcg agatccgccc aggagaagcg
     1261 ttgacgcgca tggccgccgt atccgaacag tccactccag cgcttcgtgg caagatcgcc
     1321 atctggtggg tgatcgcctc tgaggtaatg atttttggag gcgccgtggg cagttatatt
     1381 atggctcggg cggccagtcc gggatggtcg gcagaggccg cccatctgag cacctccctg
     1441 gcgacgatca acacctttgt cctgctgacc agcagtatga ccatggcgat ggcctttgcc
     1501 gccaaccaaa aggatgatcg gcgtagggtg caaaaattcc tgctgctcac catcctgctc
     1561 gggctggcgt tcctcggaat caaagcctat gagtatacct cccacatcat ggagggcgcc
     1621 gcaccttgga gcggcccctt ctgggccttt tattacctgc tgacgggact acacgcgcta
     1681 cacgttattg ccggggttat tgtgaacgtt atcctgttgc tggcagcagg gaaggggttg
     1741 agcactgggt atcggatcga gattgcggga ttgtactggc actttgtcga catcgtctgg
     1801 atctttctct tcccgcttct ctatctttcg tactgaaatg tattatgggg cttccgcaaa
     1861 ttgtgcgggt gactatcact cgtcaacgcg ccatccggat cgtaggggcg ctgcttgctg
     1921 cacccttctc aggattacgt actgccgggc agggcaaacc ctgcccctac aataccgatg
     1981 ccattcacta taccaatggg gaaatgaccc tcgcgaccca cacgaaaccc gaaagagcct
     2041 atctcataat gtctcgtctt gctgcacgta ccgccgagga gcggtgacgc ccttgtctgg
     2101 aggaggcccc aagtgaccac tgaacgggtt caccataact atgtggctat ctgggtgtgg
     2161 ctgatcgtgt tgatggtcgc cggggtcctc gcaacccgtt tgcccttggg aaagtcggcc
     2221 gtcaataacc tgatctttgc cattgcagcc ataaaggcag cgctggtcgc gctgaactac
     2281 atgcacctga aatctgagaa ttggctgatc tacgccctgg cgatcgttcc agtgctgctc
     2341 gtcattgcca tgactttagt tctttttcca gacatcgtct ttcaccgttg agcgtgtctt
     2401 atggtattct cgtataacca tggctaaagg cgcgaacgtc atcatcatcg gcggcgggat
     2461 tatcggcgta gcctgcgcct acgctctgac gcgcgctcac gtcaatcgtg tcgtcctgat
     2521 cgagcgtgga cagcttgggc gggaggcatc gcgggcttca gcaggcatgc tggtgcctca
     2581 gggagaggcg gaaggaccag ggcccttcct cgacctctgc ctcgccgcga gggatcgcta
     2641 ccggacgctg gccgatgaga ttcgggacgc gaccggggag gacattgagt attgcaagtg
     2701 gggacttctc tacctcctcg atccgactga gcatgaagcg gccaaaggac gagcagcctg
     2761 gcaacgggct gccggactgc gtgtggagga gctttccggg cgtgaggtgc gtgccatgga
     2821 gcccgtcctg aatcccgaga tcggaggcgc gctcttcttt ccggatgagg cccatatccg
     2881 accc
//
LOCUS       DATMXI010000216         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_198892, whole genome shotgun sequence.
ACCESSION   DATMXI010000216 DATMXI010000000
VERSION     DATMXI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198892"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VN444_04045"
     CDS             <1..464
                     /locus_tag="VN444_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWR21015.1"
                     /translation="RAKARRLKAEHNIGMVVIDYLQLISGRSRSENRQQEVSEICRSL
                     KAMAKELKVPVVALSQLARRTEERERPQLSDLRESGAIEQDSDVVIFLYRPSYYQVRK
                     AGAEPDTKTEIIIAKQRNGPTGTVEMAFLREYVKFGALDLVHQGPDETEET"
     gene            561..1955
                     /gene="radA"
                     /locus_tag="VN444_04050"
     CDS             561..1955
                     /gene="radA"
                     /locus_tag="VN444_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWR21016.1"
                     /translation="MAKSLSHYLCQSCGYQTARWMGRCPDCGEWGSLLEERAASERHR
                     GPGDGASSVLHLATPITAVDISTLHRMSTGLTELDRVLGGGIVPGSFVLLSGDPGIGK
                     STLLLQASMQIAQGEGIVLYVSGEESLQQTRSRASRLGPLSDRLLLLAETSLQVILDQ
                     TDRIRPTILVIDSIQTIASEELESPPGSFSQVREAAVRLMAMAKKKGISTVVIGHITK
                     EGAIAGPKTMEHLVDAVVFMEGEGHQIHRLLRAVKNRFGPTPEVGVLEMTAAGLQELV
                     NPSEVFLSQRPSGASGSVVIPTLEGSRPLLVELQALVAAPSAAIPRRVFNGVDSNRGV
                     LLLAVLEKRLGLPLSACDVYVNVVGGVRVGETAADLGMAAAILSSARDLLLDPGLCVF
                     GEIGLTGEVRAVPMAERRLEEAARLGLSHCLMPRHNLNRTQPDCSSLRVSGLCTLEEL
                     AEKLETRQAAKYLD"
BASE COUNT          400 a          542 c          677 g          418 t
ORIGIN      
        1 tgcgggcgaa ggcgcggagg ctgaaagccg agcataacat cgggatggtg gtgattgact
       61 acctccagct tatctcgggt cgttcccggt ccgagaatcg ccagcaggaa gtctcagaga
      121 tctgtcggag tctgaaggcg atggccaagg agttgaaggt cccggtggtg gcgctgtcgc
      181 agctcgcgcg gcgaaccgag gagcgggagc gcccgcagct atcggacctc cgggaatccg
      241 gggccattga gcaggattcg gatgtcgtga tcttcctgta ccggcctagc tactatcagg
      301 tcagaaaggc cggggctgag ccggacacca agaccgagat catcatcgcc aagcaacgga
      361 acggcccgac cggaaccgtg gagatggcct tcctcaggga gtatgtcaaa ttcggcgctc
      421 ttgacctcgt ccatcaaggg ccggatgaaa cggaggagac atgatgggtg gcggttcaac
      481 gttcaaagtt tacgattcaa cgttggacat tgcacgttga acgttgaacg gttcggtgtg
      541 ccaccgcgga gagatgagcg atggcgaaaa gcctgagcca ttacctctgt cagagctgcg
      601 gctatcagac cgctcgctgg atgggtcgct gccctgactg cggagagtgg ggaagcttgt
      661 tagaggagcg cgccgcgagc gagcggcatc gtggccctgg cgacggggcg tcctcggttc
      721 tacatcttgc gacaccgatc accgccgtag atatcagtac gctccaccgg atgagcaccg
      781 gtttgacaga gttggatcgg gtgctgggag gtgggatcgt tcctggctcg ttcgtgctct
      841 tgagcggtga tccggggatc gggaagtcaa ccctgctcct tcaggcatca atgcagatcg
      901 cgcaggggga ggggatagtc ctgtatgtct ctggtgagga atcgcttcag cagacccgat
      961 ccagggcgag ccggctgggt cccctctccg atcgtctcct gcttctcgcc gagacctccc
     1021 tccaggtgat tctggatcag accgatcgaa ttcggccaac cattctggtg atcgattcca
     1081 tccagactat tgcctcggaa gaactggagt cccccccagg tagtttcagc caggttcgcg
     1141 aagctgccgt tcggctgatg gcgatggcca agaagaaggg aatcagtacc gtggtcattg
     1201 gccatatcac gaaggaaggg gctatcgcgg gtccgaagac gatggagcac ctcgtagacg
     1261 cggtcgtatt catggagggg gaggggcatc agatccatcg tctgctgcga gccgtgaaga
     1321 atcgttttgg ccctactccc gaggtcggtg tgcttgagat gacggcggcc ggactgcaag
     1381 aactcgtaaa tccgtcggag gtctttctct ctcagcgccc ctcgggggcg tcgggctcag
     1441 ttgtcattcc gaccctggag gggagtcggc cacttctcgt ggagctgcag gcgttggtgg
     1501 cggcaccaag cgctgcaatt cccagacggg tgttcaatgg ggtagacagc aaccggggcg
     1561 tcctgctgct ggctgtgttg gaaaagcggc tcggtctgcc gctcagcgcg tgtgatgttt
     1621 acgtaaacgt tgtggggggc gtcagagtag gagagaccgc cgccgatctt ggcatggccg
     1681 cggcgatcct ctcaagcgcc agggaccttc tactcgatcc cgggctctgc gtgttcggcg
     1741 agatcggtct aacaggtgag gtgcgggcgg tgccgatggc ggagcgacgg cttgaggagg
     1801 ccgcccgcct ggggttgtcc cattgcttga tgccccggca caacctcaat cggacccagc
     1861 ctgactgttc ctctttacgg gttagtggac tatgtaccct ggaggagttg gcggagaagc
     1921 tagagacacg gcaggcagca aaatatcttg actagtcaga gaatccctgt catagtctat
     1981 agttaatgat gtatagtacc gggacaaaag tagtctatcc cacccatgga gttgggt
//
LOCUS       DATMXI010000217         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_69376, whole genome shotgun sequence.
ACCESSION   DATMXI010000217 DATMXI010000000
VERSION     DATMXI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69376"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1361
                     /gene="galT"
                     /locus_tag="VN444_04055"
     CDS             363..1361
                     /gene="galT"
                     /locus_tag="VN444_04055"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HWR21017.1"
                     /translation="MPQLRKDPITSRWVIISTERGKRPSDFSTAPPPRQLGFCPFCPG
                     NEDKTPPEIMAYRKDGNLPDRKGWSLRVIPNKFPALQIEGDLDREGEGIYDKMHGLGA
                     HEVIIESPNHNDTLSAMSEEQVEDVLWAYRDRILDLKKDDRFRYILIFKNHGEQAGAS
                     LEHPHSQLIATPIVPKRACEEVDGAKAYFKYKDRCIYCDIIRQELQQESRVIALNDEF
                     VAIAPFASRFPFETWILPRNHEPFFQDSKKVDMVSCSRILKEVLGRMDQVLTNPPYNY
                     LIHSAPLQETEVNHYHWHLEIMPTLVKVAGFEWGTGFYINPVPPEDAAKYLREARL"
     gene            1631..1969
                     /locus_tag="VN444_04060"
     CDS             1631..1969
                     /locus_tag="VN444_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWR21018.1"
                     /translation="MKKIEAIIKPFKLDEVKSALAEVGIQGLTVSEVKGFGRQKGHTE
                     LYRGSEYTIDFLPKVKIEVVVPDDKCDKVVETILSSAKTGRIGDGKIFIVSIDEVVRI
                     RTGERGEAAI"
     gene            2055..3470
                     /gene="glnA"
                     /locus_tag="VN444_04065"
     CDS             2055..3470
                     /gene="glnA"
                     /locus_tag="VN444_04065"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWR21019.1"
                     /translation="MTPKDVITLANERGAKIVDLRFIDMPGLWQHFSISRRELTEDLF
                     TDGIGFDGSSIRGFQAIHESDMLLIPDPSTAMMDPFMVVPTLVLICNIMDPVTRQLYS
                     RDPRYIAQKAEAYLTSTALADTAYVGPELEFFVFDDVRFDQSYQHGYYFIDSEEGFWN
                     SGKDEKPNLGYKPRYKQGYFPVPPMDKLHDLRSEMVLAMESVGIPIEVHHHEVATAGQ
                     CEIDMRFDTLTKMADRVQWFKYCVKNTARKYGKTATFMPKPIFQDNGSGMHVHQSLWK
                     GGKNTFWELGGYGDLSATARYYIGGLLKHAPALCALIAPTTNSYRRLVPGYEAPINLV
                     YSQRNRSAAARIPLYSKSERAKRIEFRTPDPSCNAYLAFSAMLMAGIDGIQKKIDPGQ
                     PVDKDLYELEPEETKDIRQLPGSLGDVLDALEADHEFLLKGDVFTPDLIDTWIKYKRK
                     NEVDPIRLRPHPWEFALYFDI"
BASE COUNT          891 a          994 c         1145 g          799 t
ORIGIN      
        1 gtacggcctg gctatcgggc atgtcgaaac aagcctcacg tgggagagcg aggggatcag
       61 gttcttagaa gtcgttgtag tggatgaaca gagccacatg agcaacgcat tgcgagcacg
      121 tgtcgcggtc acccgctggt gagcgtcggg tttcttggat tgcgcaggcg cgacacctct
      181 cgggaaacct ggtcacggtg ggatgtggcg gcgccaagca gcggttgagc atggtggacc
      241 ttccaggaaa tggcgcctct cactgctttc agtaactacc cattgacatt cccaggctcg
      301 tgatttaatg tgatacgctt gtcaagcgag gggatctcgg gacaaaggag gaaaacaacc
      361 ggatgccgca gctccgcaag gatccgatta cttcgcgatg ggtcattatt tcgacagagc
      421 gagggaagcg tccatcggac ttttccactg ctccgccacc gaggcagttg gggttttgcc
      481 cgttctgtcc agggaatgag gataaaacgc ctcctgagat catggcctat cggaaggacg
      541 gtaacctgcc tgatcgcaag ggatggagcc ttcgcgttat accaaataag ttccccgcac
      601 tccagatcga gggagacctg gatcgggagg gtgaagggat ctacgacaag atgcacgggc
      661 taggtgctca cgaggtgatc atcgagtctc ccaaccacaa tgacacgctc tccgctatgt
      721 cagaggaaca agtagaggat gtcctgtggg cctaccggga ccggatactc gatctcaaga
      781 aagacgatcg atttcgctac attttgatct ttaagaacca cggtgagcaa gccggcgcct
      841 ctttggagca ccctcattct cagctcattg ctacccctat cgttccgaag cgggcctgtg
      901 aagaggtgga tggcgccaag gcctacttca aatacaagga tcgctgcatc tactgcgaca
      961 ttatccggca ggaactgcag caggaaagta gggttatcgc gttgaatgat gagttcgtgg
     1021 ctatcgcccc cttcgcatcg cgctttccgt tcgaaacctg gatcctgcct aggaatcatg
     1081 agccattctt ccaggactcg aagaaggtag acatggtgag ttgctccagg attttgaagg
     1141 aggtccttgg caggatggat caggtgctca ccaacccccc ctacaattac ctgattcaca
     1201 gcgctccgct ccaggagacc gaggtgaacc attatcactg gcatcttgag attatgccga
     1261 ccctcgtcaa ggttgcaggc ttcgagtggg ggaccggttt ttacatcaat cctgtcccgc
     1321 cggaggatgc ggccaagtat ctccgggagg ctcggctata gttgcataaa aaaaggtcag
     1381 ccaagcaaag gtgtgcgtaa agttttggca aaaacggagg ctcaggcggc ggcgtaatga
     1441 ctaggaaggt ccaaaggatt gcgggtttag tatgccgaca gcgcaatggc gcagggttaa
     1501 agatgttagg agccttggca tgcgatttgc tcgtttaggc ggcgggtgca aagactagca
     1561 gtggtgtctg gagtgggggc gtaagacccc cggcggggag gcaaacgcct tcagagacgg
     1621 aggatgtcgg atgaagaaga tcgaagcgat cattaagccc ttcaagctcg acgaggtgaa
     1681 gagcgctctg gctgaggtgg ggatccaggg cctcaccgtg agcgaggtca agggtttcgg
     1741 ccggcagaaa ggccacaccg aattgtacag aggcagtgaa tacactatcg actttctacc
     1801 caaggtgaag atcgaggtcg tggttccgga cgacaagtgc gataaggtcg tggagacgat
     1861 cctgtcctcg gcgaagacgg gtcgaatcgg agatgggaag atctttatcg tgtcgatcga
     1921 tgaggtcgtc cggatcagaa ccggcgagag gggtgaggcg gcgatttgag gatgtgcggt
     1981 gaaggagaat tccaccgctt cataaactgt tcaacgacga atcattcgac ccaagaagga
     2041 ggaaaggcag ccctatgaca ccgaaggatg tcatcacgct ggcgaacgag cgaggcgcga
     2101 agatcgttga cctcagattc atcgacatgc ctggtctgtg gcagcacttt tctatttcca
     2161 ggcgagaatt gacggaagat ctgttcacgg acggaatcgg gttcgatgga tcctccattc
     2221 gaggcttcca ggcaatccat gagtcggaca tgctcctgat tcccgatccg tcgacggcga
     2281 tgatggatcc cttcatggtc gttcccaccc tggtcttgat ctgtaatatc atggatccag
     2341 tgacaaggca gctctacagc cgagatccgc gatatattgc gcaaaaggcc gaggcgtacc
     2401 tcacgtcaac agcgcttgca gataccgcct acgttggtcc tgaactggag ttcttcgtct
     2461 ttgacgacgt tcggttcgat cagagctacc agcatggcta ctacttcatt gactcggagg
     2521 aggggttttg gaactccggg aaggacgaaa agccgaacct gggctacaag cctcgctaca
     2581 aacagggcta tttccccgta ccaccgatgg acaagctcca cgacctccgc tcggagatgg
     2641 tgttagcgat ggagtctgtc gggattccga ttgaggtcca ccaccatgaa gtcgcgacgg
     2701 ccggtcagtg cgagatcgac atgcggttcg acacgctcac caagatggcg gacagggtac
     2761 aatggttcaa gtactgtgtg aagaacaccg ctcgtaagta cggtaagacg gccaccttca
     2821 tgcccaagcc gatcttccag gacaacggct ccggcatgca cgtccaccag agcctctgga
     2881 agggtggcaa gaataccttc tgggaactag gagggtatgg agatctgtcc gcgacggctc
     2941 gctactatat cggtggattg ctcaagcatg cgcccgcgct ctgcgccctc atcgccccaa
     3001 ccacgaattc gtaccgtcgg ctcgtgccag gctacgaggc gccaatcaac ctcgtgtaca
     3061 gccaacggaa ccgatcggcc gcggccagga tccccctcta ctcaaagagc gagagggcaa
     3121 agcggatcga atttcggacg cccgatccca gttgtaacgc ctatctggcc ttcagcgcga
     3181 tgctcatggc cggtattgac ggcatccaga agaagattga ccctggacag ccggtcgaca
     3241 aagatttgta cgagctcgag ccggaagaga cgaaagatat caggcagtta ccgggcagcc
     3301 tgggggatgt cctggacgct ctggaagccg accacgagtt cctcctgaag ggcgatgtct
     3361 ttaccccgga tctcatcgac acctggatca agtacaagcg gaagaacgag gtcgatccga
     3421 tccgcctccg ccctcatccc tgggagttcg ccctctactt tgacatctaa gcattctcgg
     3481 ttcttctttc tgaaaacggc cccggatgat ctatccgggg ccgttttgct tttttcctga
     3541 gcctcaggac gtggctgaac tcgatgcagg ttgcacgcgc cagagctgca tgctagtatt
     3601 ttgtcgattt tcagtgtaac tcatgtcggc cctgggctga gcacagagga gggaagagat
     3661 gaagcgtgca ggaagttggt tagttctgat cggacttggg gtcgcgatca caggctgtgc
     3721 aacgacggag ccgcggccca atgtgtacgt ttaccctaaa caagggcaga cggtggagca
     3781 gctcgaccga gatcggtttg agtgccagga gtgggcgaga cagcagacc
//
LOCUS       DATMXI010000218         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_129073, whole genome shotgun sequence.
ACCESSION   DATMXI010000218 DATMXI010000000
VERSION     DATMXI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129073"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2229)
                     /locus_tag="VN444_04070"
     CDS             complement(199..2229)
                     /locus_tag="VN444_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWR21020.1"
                     /translation="MNTRIRTALLPLLLIIGWAVMPAQAQPPPPPDLTELPLEQLMMI
                     KVTTVSKREEILFNTAAAAYVITGEEIHRSGVRSIPEALRMAPGVDVAQLDANKWAIS
                     IRGFNGRFSNKLLVLIDGRSVYTPLFAGVFWDVQDTLLEDIDRIEVIRGPGGTLWGAN
                     AVNGVINIITKKAGVAQGGYVEGGGGTEERGFGSVRYGGQFAEGGFYRAYAKYFRRDR
                     FITPAGQDAADDWETYRGGFRTDWDLSADDSLTVQGDMYRGTYGQTTMIASLFPPFSE
                     TSEPTNTFSGGNVLARWRHRLTERSETILQLYYDRTHRNESLFREDRDTVDLDFQHRV
                     GLGARHDVVWGVDARLSFDDIEGSSTISFTRSNRMFHRVGGYIQDQITLIADRLYLTL
                     GTKVEHALFSGFQVQPSARLLWTPADGHAVWAAVSRAVRTPSRAEVDVRSDALTVPPD
                     PTDPSGLPTLFRVAGSHDFGSEEVIASEIGYRVKPTDWVMIDITAFYNIYNNLRTTEP
                     GTPFLSSSPAPPHIEVPLRVDNKLAGRTVGVEIATTWRPVSFWKLQLHYSFLDIHLDP
                     DPSSRDTVTKTDEGLSPRHQVQLLSHLNLPWRLQFDTAAYFVDRLPTFDLRRYLRLDV
                     RLGWRPTQAFEINLVGQNLLEGSHREFGTILGDIINTTRVQRSVLVYATWRF"
     gene            complement(2440..>2792)
                     /locus_tag="VN444_04075"
     CDS             complement(2440..>2792)
                     /locus_tag="VN444_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR21021.1"
                     /translation="AMITMYAAVATRTSEIGALRALGFRRRSILIAFLAEALFLGLTG
                     WLLGLVFASAMQLVQISTMNWQSFSELAFRFTLTPEIVWKSLVFALVMGLSGGFLPAV
                     RAARLKIVDALRAA"
BASE COUNT          553 a          824 c          911 g          504 t
ORIGIN      
        1 gagcaatgac gcatcccaat gtctgtgctt ggggagcgga tcgcatgagc ctgcgcgaca
       61 ctgtgacggc tagtgcttaa ttgcaataat gcccgtaacg tcattcccgc agtccttaag
      121 cgggaatcca ttggttttac cggatacccg ctttcgcggg tatgacggtt tatgtacgct
      181 aactttcgca attaagcgct agaatcgcca tgtggcgtac accaatacgc tccgttgcac
      241 cctggtggtg ttgatgatgt cgccgaggat cgtgccgaac tcccgatgcg agccctccag
      301 caggttctgg ccgaccagat tgatctcaaa cgcctgcgtc ggacgccagc cgaggcggac
      361 atccagccga aggtagcgac ggagatcgaa cgtggggagc cggtccacaa agtaggccgc
      421 tgtgtcaaac tgcaggcgcc agggcagatt cagatgcgag aggagctgaa cctgatggcg
      481 ggggctcagc ccctcgtcgg tctttgtgac ggtgtcgcga cttgaagggt cgggatccag
      541 gtggatgtcg aggaatgagt agtggagctg tagtttccag aagctgacgg gacgccaggt
      601 ggtggcaatc tcgacgccga cggtgcggcc ggccagcttg ttgtcaaccc ggagcgggac
      661 ctcgatgtgc ggcggcgcag ggctggacga gaggaacggg gttccgggct cggtggtgcg
      721 gagattgttg tagatgttgt aaaaggcggt gatgtcgatc atgacccaat cggtcggttt
      781 gacccggtag ccgatctccg acgcgatcac ttcctcggat ccgaagtcgt ggctgccggc
      841 gacgcggaaa agggtcggta gcccgctcgg atcggtcgga tcggggggaa cggtgagcgc
      901 atcggaccgc acatccacct cggcccggga cggggttcgg acggcgcgag agacggccgc
      961 ccagaccgcg tgtccgtcgg cgggcgtcca gagcagcctg gcgctcggct gcacctgaaa
     1021 cccggaaaag agggcatgct ccacctttgt gccgagcgtc agatacagcc ggtcagcgat
     1081 cagggtgatc tgatcctgaa tgtagccgcc cacgcggtgg aacatgcgat tcgacctggt
     1141 aaacgagatg gtggaactcc cctcgatgtc atcgaaggac aggcgcgcat cgacgcccca
     1201 cacgacatcg tggcgggccc ccaggccgac acgatgctga aagtcgaggt cgaccgtatc
     1261 ccgatcctca cggaacaggc tttcgttccg atgtgtcctg tcgtaataga gttgtaagat
     1321 cgtctccgat cgctcggtaa ggcgatgccg ccatcgagcc agcacgttgc cgcccgaaaa
     1381 ggtattggtg ggttcggagg tctcggagaa aggggggaag agcgatgcga tcatcgtggt
     1441 ctggccgtac gtgcccctgt acatatcgcc ctgcaccgtg aggctgtcat cagccgagag
     1501 gtcccagtcg gtgcggaagc ctcccctata ggtttcccaa tcatcggcgg cgtcctgacc
     1561 ggccggggtg atgaaccggt cgcgcctgaa gtatttcgca taggcccggt agaaaccccc
     1621 ttccgcaaac tgaccgccat agcggacgct cccgaatcct cgctcttcag tgccgccgcc
     1681 gccttcgacg tatccgccct gtgccacgcc ggccttcttg gtaatgatgt taatcacgcc
     1741 gttgaccgca ttcgcgcccc acaaggtgcc accgggacct cggatgacct cgatccggtc
     1801 gatatcctcg aggagggtgt cctgtacgtc ccagaagacc ccggcgaaga ggggggtgta
     1861 cacactccgg ccatcgatga ggaccagcag cttgttcgag aagcggccgt tgaacccgcg
     1921 gatagagatg gcccacttgt tggcgtcgag ctgcgcgacg tcgacccccg gcgccatgcg
     1981 caacgcttcg gggatgctcc gtacgcctga gcggtggatc tcttcgccag taatcacgta
     2041 ggccgccgca gcggtgttga agaggatctc ctcccgcttg gagacggtgg tcaccttgat
     2101 catcatcagt tgctcgagcg gcagttccgt gagatccggt ggcggtggag gctgggcctg
     2161 ggcgggcatg acggcccacc cgatgatgag cagcaacggc agcagcgcag ttcggatgcg
     2221 cgtgttcacg gtccctccat tcttcgccaa ggagtggaag cggagccgag tagagcctat
     2281 aaaaaactca tagtatgaca aacggggcta ctcttcaagc gtaaacggcg cagagaagcg
     2341 ggaagggtaa ggaagcgcgt cagtctacca gtcgatgctg gagcgcatat cggatcagtt
     2401 ccgcgttggt ctcgaccccg agatcacgcg gcgcagactc tacgccgccc gcagggcgtc
     2461 gacgatcttc aggcgcgccg cgcgcaccgc gggcaggaac ccgccgctca gccccatgac
     2521 gagcgcgaag acaagcgatt tccagacgat ctccggcgtg agcgtgaacc gaaaggcgag
     2581 ctccgagaac gactgccagt tcattgtcga gatctgcacc agttgcatcg ccgaggcgaa
     2641 caccagcccc agcagccagc cggtcagccc gaggaagagc gcttctgcga gaaacgctat
     2701 aaggatcgag cggcgccgga atccgagggc gcgcagcgcg ccgatctccg acgtgcgggt
     2761 cgctaccgcg gcgtacatgg tgatcatcgc ac
//
LOCUS       DATMXI010000219         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_323385, whole genome shotgun sequence.
ACCESSION   DATMXI010000219 DATMXI010000000
VERSION     DATMXI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323385"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VN444_04080"
     CDS             <1..294
                     /locus_tag="VN444_04080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR21022.1"
                     /translation="LYDSFANVTLEAAAAGLPILTSRQNGASELIEDGRSGLIVSSPT
                     ELSEIGGKLTELFDMTKRRALGWAARDVAIQFPVRRYAQGTMQVYQAIMEEKS"
     gene            291..1505
                     /locus_tag="VN444_04085"
     CDS             291..1505
                     /locus_tag="VN444_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWR21023.1"
                     /translation="MMNKALASGLETGGRVDYPDLVFMLGLSIMILFLPLSETLKNVG
                     YALALAGWIIKKAVNRRFQIRMTPFGGVLSLYLLTSLLSAAFAVDPREGLRGAWDVFR
                     PLSLFVMMVNDVETLPKIRLCLLLFIISTTIGVAWGLFDNLSGRDFRLEIRSLGYPNH
                     TATYLAMMLAVLISLLLLMEWSTRVKMLVWTVAGATMLALFLTYSRGGWIAFAACLLF
                     LSISLKQWKPIVAIALLGTGILVGLQVTGRLWTRQITMLAHLDQDENALQRLRMWRGS
                     VLSLKDRPLLGVGPRNFKNLDHERYGLPRFDHAHSLFFTVLAERGLLGFLALMAVLAC
                     YLYEAIKLRPLQDVLSRALRHAAIGSFIALVVAGTVNTTLRGEVAIALCSFMALALAA
                     AKGATSAGQDPS"
     gene            1526..>2349
                     /gene="rfaQ"
                     /locus_tag="VN444_04090"
     CDS             1526..>2349
                     /gene="rfaQ"
                     /locus_tag="VN444_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HWR21024.1"
                     /translation="MMTTVSNGAPERVLIIKLRYLGDVLLSTPVLAALRTAFPQARLS
                     MLVNPGTEAMLAENPHLDEVLIVERGRSPLRQLRFVAALRRRRFDLIVDLTDGDRAAI
                     LSRLIGAAVRVGFNRGDRWRGRLYTHLVPVRQQPVSMVRQHLMALETLGIPVALSPPV
                     LNVRAADESAALAALAAIEITPAERFVAVHPGARWWFKSWPADRFAGLIDYIQGKLGL
                     KVVLLGGDQEHGIAKAILDYVETGCRSLVGRLTLLELAALLRRAALFVGNDSGPMH"
BASE COUNT          396 a          668 c          754 g          531 t
ORIGIN      
        1 ctctacgatt ccttcgcgaa tgtgaccctg gaggcggcag cggctggtct tcccattctg
       61 accagtcgcc agaatggcgc gtcggagctg atcgaagacg gacggagcgg attgatcgtc
      121 agcagcccga cggagctttc ggagattggc gggaagctga cggaactgtt cgatatgacg
      181 aagaggcgtg cgctgggttg ggcggcgcgc gacgtggcca tacagtttcc tgtgcgtcgg
      241 tacgcccagg ggacaatgca ggtgtatcag gcgattatgg aagaaaagtc atgatgaaca
      301 aggcattggc gagcggcctg gagaccgggg gccgggtgga ctatcccgac ctggtcttta
      361 tgcttggcct gagcatcatg atcctgtttt tgcctctttc cgagaccctc aagaatgtcg
      421 ggtatgcctt ggcccttgcg gggtggatca taaaaaaggc cgtcaaccgc cggttccaga
      481 ttcgcatgac accctttggg ggcgtcctga gcctgtacct cctgacatcg ctgctcagtg
      541 ccgcatttgc cgtcgatccg cgagaggggc tccgcggggc gtgggatgta ttccgtcctc
      601 tttcactctt cgtgatgatg gtcaacgatg tggagaccct ccccaaaatc cgcctatgcc
      661 tcctgctctt tattatctcg accacgatcg gcgttgcctg gggcctcttc gacaacctga
      721 gcggtcgcga ctttcgctta gagatcagat cgctcggcta tccgaaccat acggccacct
      781 acctggcgat gatgcttgcc gtcttgatca gtctgttgct cctgatggaa tggtccactc
      841 gagtgaagat gctggtctgg actgtcgccg gagcgacgat gctggcgctt tttttgacct
      901 attcgagagg gggatggatc gcctttgcag cctgtctgct gttcctctct atttctctca
      961 agcagtggaa gccgattgta gccatcgccc tgctcgggac ggggatcctg gtcggccttc
     1021 aggtcacagg caggctgtgg accaggcaga tcaccatgct ggctcacctt gaccaagatg
     1081 aaaacgcgtt acagcggttg cgcatgtggc gtgggtcggt tctcagcttg aaagaccgcc
     1141 cgctcctggg cgttggtccc aggaacttta agaatctcga tcatgagcgg tatggtttac
     1201 cccgcttcga tcacgcccat agcctatttt tcaccgtact ggcagaacga ggtctcctgg
     1261 ggtttcttgc cctcatggcc gtgttggcgt gctatctgta tgaagcgatc aagctgaggc
     1321 ctttgcagga cgttctcagc agggcccttc ggcatgccgc gataggcagc ttcatcgccc
     1381 tcgttgtggc ggggacggtc aacacgaccc tccgtggaga ggtagccatt gcgctctgtt
     1441 cgtttatggc gctggcgctg gctgctgcga agggggcaac atccgccggg caggatccgt
     1501 cgtgatcgac gccgggagga acactatgat gactacggtg agcaacgggg cgccggagcg
     1561 agttctgatc atcaagctcc ggtatctcgg ggatgtgctg ctgagcacac cggttctcgc
     1621 ggcactcagg acagcctttc ctcaggcccg tctctcgatg ctggtcaacc ctggtaccga
     1681 agccatgctg gcggagaacc cgcacctgga cgaggtgctg atcgtggagc ggggccgatc
     1741 gccgcttcgc cagcttcgat tcgttgcggc gctgcgtcgg cgacgctttg atcttattgt
     1801 cgatctgacg gatggcgacc gagcggcgat cctgagtcgg ctcatcgggg ccgccgtcag
     1861 agtgggtttc aatcgaggcg accgctggcg cggccgactg tacacccatc tggtcccggt
     1921 gcggcagcag ccggtttcga tggttcgcca gcacctgatg gcgctggaga cgctgggaat
     1981 tccggtcgcg ctgtcgccgc ccgtgctgaa cgttcgggct gccgacgagt cggccgccct
     2041 cgccgccctc gcggccatcg agattacacc tgccgagcgc ttcgtggcgg tgcatcccgg
     2101 ggcccggtgg tggttcaaga gctggcctgc cgatcggttt gccggtctca tcgactatat
     2161 tcagggcaag ttggggctga aggtggtgct gctgggggga gatcaagaac acggcatcgc
     2221 caaggcgatt ttggactatg tagaaaccgg ctgtcggtca ttggtcgggc gcctgacgct
     2281 cctggaactg gctgcgctgt tgcggcgggc tgccctcttt gttggaaacg acagcggacc
     2341 gatgcatat
//
LOCUS       DATMXI010000220         6009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_124125, whole genome shotgun sequence.
ACCESSION   DATMXI010000220 DATMXI010000000
VERSION     DATMXI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124125"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VN444_04095"
     CDS             <1..314
                     /locus_tag="VN444_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWR21025.1"
                     /translation="PDALTLPDVPKAGHHVFNQYVVRTRRRDELHDFLASRGIGTAVY
                     YPKPLHMQACLAFLGYREGDFPEAERAARETLALPVYPELTVQMQDRVIESIDTFFKG
                     R"
     gene            849..1205
                     /locus_tag="VN444_04100"
     CDS             849..1205
                     /locus_tag="VN444_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21026.1"
                     /translation="MAEAIQLRVATFFPRPCVFMLLAFYLTAASIVLFQQIDRALAAE
                     PTLDRGVKPIQCGVILYYLILVFNLSMTFDAGEMLRGIIGITGVLLYIPIVWMVFTRY
                     WQWSAAHRYVSRGALL"
     gene            1202..2203
                     /gene="hpnH"
                     /locus_tag="VN444_04105"
     CDS             1202..2203
                     /gene="hpnH"
                     /locus_tag="VN444_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWR21027.1"
                     /translation="MRFPLHITTDMLKHQIRHGLKGSKRYPFVLMLEPLYTCNLACLG
                     CAVERHTGKIEDRLTLEECLRAADDSGAPVVSICGGEPTIYPELKGLVEGMIARKRHI
                     YLCTNGLMLDRTVYGLIAPHNRLTINIHMDGLRRTHDQVCDREGVFDKALDMIKEGKR
                     LGYRVTTNTTIFKETDLDEVEALCHLLREHQVDGMLLSPGYSYASAESDFFLAREEIH
                     HKFKKVLELSKQYRLISTPMFLEFAAGLREYPCSPWSTVTYTPEGWRGPCYLIGEKYF
                     RTFEELWQSTDWDYWESRQDRRCYNCKMHSGFEASVVRGLRNSPKDMLRMAIWNFLE"
     gene            2209..4197
                     /gene="shc"
                     /locus_tag="VN444_04110"
     CDS             2209..4197
                     /gene="shc"
                     /locus_tag="VN444_04110"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWR21028.1"
                     /translation="MPVSSERNWQVEMMRTDPRADRDPLDEAIEQARSRLLAMQDPTG
                     FWVAELEADSTLTSEYIMLRYLLGKVDKTKQRKAAAYLLDTQLPDGGWNLYHGGSSHL
                     STTVKAYFALKLCGYSSEEPFMRRAKEVILSQGGLLQANVFTKFALALFGQFDWHDVP
                     AMPIELFLLPKHSFFNMHEISYWSRTVLTPLMIIFAHKPVTPVPAGADLMELRGEPRP
                     KHADWFPGDSRLFSKRNLFLAADRLLHWYEWRPISFLRQAAMERATTWMLRRMGEGGL
                     GGIYPAMANSIIALRCLGYEVEHSLVARAFKQIEALEVEDDRTLHIQPCLSPIWDTCL
                     AVNALAASGLPADHSALVSACEWLLSKQTRTVGDWKVKAPDAEPGGWYFQFENEFYPD
                     VDDSAVVMTALIKTILPDEAAKGTALRQALKWVLPMQSSDGGWAAYDKDNNKLFLNEL
                     LVADHKALLDPSTADLTGRMIEMLGHLGWTSTAPAAQRAIAFLKREQEPQGCWFGRWG
                     VNYLYGTWAVLAGLQAIGEPMDQPYIRRAVDWLIDHQNPDGGWGESCHSYEDPQTAGQ
                     GPSTPSQTAWALLGLLHAGCVQHSAVKSGIDYLLRTQCADGGWEEQEFTGTGFPRVFY
                     LRYHLYRLYFPLWALSLYRTLLQQAASDHDENGATRPD"
     gene            4169..4987
                     /locus_tag="VN444_04115"
     CDS             4169..4987
                     /locus_tag="VN444_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21029.1"
                     /translation="MKTARPARTSTVCGAPLAIFVATQAEMKPIATALQPTDPSASRS
                     DLIVRIEVGGRDLLLVKTGVGPDRAEAAARHLFEAEPVVAALSLGVAGGLSPQVQTGD
                     LIVGDRVILRRGSGRALFGEEDARPENFPCDSSLQEAALTVIRRLGSQYYLGSILTVD
                     RIVLTPKEKRLLAAESGAMALDMESAAIASAASAYSIPFLTIRGVLDPVHEDLAIDFD
                     RFLDAEGEPSLPRLMRYLITHPFTLPRLVGLGRRTTAVCARLGRLLHELSTTLS"
     gene            5033..>6009
                     /locus_tag="VN444_04120"
     CDS             5033..>6009
                     /locus_tag="VN444_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWR21030.1"
                     /translation="MGNPDLSKLSIDRTATRVSHAQRRWWPWAVGLALLVALAVLGFS
                     GGLGGSVTVETASVTTAYPSQAVTMLNATGYVVAQRKAAVASKATGRLEWLGVMEGSR
                     VKKDEVVARLESRDVAAGREQAAASVKVAEANLEQAQAELRDAERNLTRWRELSQNKL
                     ISELELDTAIARADKARAAVRSSEAAIAVARANLKAADVSFDQTLIRAPFDGVVLTKN
                     ANVGDNITPFSSAVDTKGAVVTIADMSTLEVEADVAESSLSRIKVGQPCEIQLDAVPD
                     TRFAGVVNRIVPTVDRAKATVMVKIGFVDRDERLLPDMSAKVAFLEREVA"
BASE COUNT         1189 a         1801 c         1797 g         1222 t
ORIGIN      
        1 gccctgatgc gcttaccctg ccagacgtgc cgaaggccgg gcatcatgtc ttcaatcagt
       61 atgtcgtccg gacacgccga cgggatgagc tccacgactt cctcgcgtcg cgggggatcg
      121 gtacggctgt ctactacccc aagccgcttc acatgcaggc ctgcctcgcg ttcctcgggt
      181 atcgagaggg agatttcccg gaagccgagc gagccgctcg tgagaccctg gcactacccg
      241 tctatccgga gctgacagta cagatgcaag accgcgtcat cgagagcata gacaccttct
      301 tcaaggggcg atgagcgcta cctgcacaga cctatagtcc tgctggaaaa aggaacgtcc
      361 cccaccttcc cacctggagc accgtcacct tttgttgcga tgcaaggcaa gttgatagct
      421 ccctctcgtc cctggaccgt cctcgctctt tggcgacagg agcaaaataa gaaaatggaa
      481 aatagggaca gacactattc tctcaccatc tctcaaataa tagtgtctgt ccctattttc
      541 cattttaagg ttgccagcgc cgggttggat atgatatatg tgagaatctc gcgtgaacgg
      601 ccaatggccg atcagtctca gtggcccata ttgccacaat gtggtaggtc tgcgatttgg
      661 agtgcctcct acccttgggg tgattatcca ttccaatggc cggagaaccc agaaggctgg
      721 gcttcgactt cgtctctgac ccgccaggcc caccactgag ccacaactgt tcacgcagga
      781 ttcaggatga cattttgcta tatttcggat gctggatgtc agtgggctgg ttactacgag
      841 ggagctgaat ggcggaagcg atccaacttc gtgtcgctac gtttttccca agaccctgcg
      901 tttttatgtt gctggctttc tatctgacgg cggcatctat cgtcctgttt caacagatcg
      961 atcgtgctct ggcggcagag ccgaccttag accgaggggt gaaaccgatc cagtgtggcg
     1021 tcatactcta ctatctgatt ctagtcttta acctctcgat gacctttgac gccggcgaga
     1081 tgctgcgggg gataattggg ataaccggag ttttactgta catcccaatc gtttggatgg
     1141 tcttcacacg ctactggcag tggtcagcgg cccacagata tgtcagtcgt ggagcgctct
     1201 tatgagattt ccgctgcaca tcacgacgga tatgctcaag caccagatcc gccacggact
     1261 caaagggagc aagcgatatc cattcgtcct tatgctcgaa ccgctgtaca cctgcaacct
     1321 ggcctgtcta ggctgcgccg ttgagcggca caccgggaaa atcgaggatc ggttgacgct
     1381 ggaggagtgc ctgagggcgg cggatgactc gggcgctccg gtcgtctcga tctgtggcgg
     1441 ggagcccacc atctaccctg aactaaaagg actggtggag gggatgattg cccgtaagcg
     1501 gcacatttac ctgtgtacca acgggttgat gcttgatcga accgtctatg gcctgatcgc
     1561 gccgcacaac cggcttacta tcaacatcca catggatgga ctaaggcgga cccacgacca
     1621 ggtctgcgat agggaaggtg tcttcgataa ggcgctcgac atgatcaagg aaggcaagcg
     1681 gctcggctac cgggtgacaa caaatacgac gatcttcaag gagaccgatc ttgacgaggt
     1741 tgaggcactg tgccacctgc tgcgggagca tcaggtggac gggatgctcc tctcgcctgg
     1801 gtatagctat gcctcagccg agtctgattt cttcctggct cgcgaggaga tccaccacaa
     1861 gttcaaaaag gttctcgaac tatcgaagca gtatcgactg atctccaccc cgatgttcct
     1921 cgaatttgcg gccggcctac gcgagtaccc ttgctccccg tggagcactg tcacctatac
     1981 ccctgaaggt tggagggggc catgctatct catcggggag aaatacttcc ggacgtttga
     2041 ggagctctgg caaagtacgg attgggacta ctgggagtcc cgccaggacc ggcgctgtta
     2101 taactgcaag atgcactccg gcttcgaggc ttctgtcgtc cgcgggctcc gaaacagccc
     2161 gaaggacatg ctcaggatgg ccatctggaa ctttttggag tgagcgccat gccggtgtca
     2221 tccgaaagaa attggcaggt tgagatgatg aggaccgacc ctcgggccga cagggacccg
     2281 cttgacgagg caatcgaaca ggcccggtct cggctcctgg ccatgcaaga tcctacaggc
     2341 ttctgggtgg cggagttgga ggccgactcc acgctcacct ccgagtacat catgctccgc
     2401 tacctgctcg gcaaggtgga caagaccaag cagcgcaagg ccgcggccta cctgctcgac
     2461 acgcaactgc ccgatggcgg ctggaatctc taccatggtg ggtcaagcca cctgagcacc
     2521 acggtcaagg cctattttgc gctcaagctc tgcggctact ccagtgagga gccgttcatg
     2581 cgacgggcca aagaggtcat tcttagtcag ggcggcctcc ttcaggctaa cgtcttcacc
     2641 aagtttgccc tcgccctctt cggccagttt gactggcacg acgtcccggc catgcccatc
     2701 gaactgttcc tcctccccaa gcattcgttt ttcaatatgc atgagatctc ctactggtcc
     2761 agaaccgtgc tcaccccttt gatgattatc ttcgcccata agccggtcac gccggtccca
     2821 gccggagccg atcttatgga gcttcgcggg gaacccaggc cgaagcatgc cgactggttt
     2881 cctggagatt cacgactgtt ttcgaagcga aatctttttc tggccgcaga ccggctgctc
     2941 cactggtatg agtggcgtcc aatctcattc ctccgccagg cggcgatgga gcgggctacg
     3001 acgtggatgc ttcgacgaat gggggaaggc ggccttggcg ggatctatcc ggcaatggca
     3061 aactcgatca tcgcgcttcg ctgtctgggc tatgaggtcg agcactcttt ggtggccagg
     3121 gccttcaagc agatcgaagc gcttgaagtg gaagacgatc ggactcttca catacaaccc
     3181 tgtctctccc ctatatggga tacctgcctg gcggtgaatg cgcttgccgc atcgggactt
     3241 cccgccgacc actccgccct ggtcagcgca tgcgaatggc tcctctcgaa acagacccga
     3301 acggtggggg actggaaggt caaggctccc gacgccgagc ctggcggctg gtactttcag
     3361 ttcgagaacg agttttaccc ggatgtcgac gacagtgcgg tggtgatgac cgccctgatc
     3421 aaaaccattc tccccgatga ggcggcgaaa gggaccgcgc tcagacaggc gctcaagtgg
     3481 gtcctgccga tgcagtcaag tgatggaggc tgggcagcat acgacaagga taacaacaag
     3541 ctgttcctga acgagctcct cgtcgccgac cacaaggcgc tgctcgatcc gtccacggcg
     3601 gacctgaccg gacggatgat cgagatgctt ggccacttgg gttggacctc tacagccccc
     3661 gcggctcagc gcgctatcgc gttcctcaaa cgggagcagg aacctcaggg atgctggttc
     3721 ggtcgatggg gcgtcaatta cctctacggg acatgggcgg tcctggcggg ccttcaagcc
     3781 atcggagagc cgatggacca gccgtacatc cggcgagctg ttgactggct catcgaccac
     3841 cagaaccccg acggtggctg gggcgaatcg tgccattctt acgaggaccc acaaacagcc
     3901 ggccagggcc caagcacccc ctcgcaaacc gcctgggcgc tcttggggct cttacacgcg
     3961 ggctgcgtac agcattctgc ggtgaaaagc ggcatcgact atctcctccg gacccagtgc
     4021 gcggatggcg gatgggagga acaagaattc accggaacgg gattcccccg cgtcttttat
     4081 cttcggtatc atctctaccg tctctacttt ccgctctggg ccctttccct ctatcgcacc
     4141 cttctccagc aggccgcctc cgaccacgat gaaaacggcg cgacccgccc ggactagtac
     4201 ggtctgcggc gcgccactcg ccatcttcgt ggcgactcag gcagagatga agcccatcgc
     4261 cacagcctta cagccgactg atccgtcagc ctcccgatcg gacttgatag tccggattga
     4321 ggtagggggc cgcgatctgc tgctggtaaa gaccggagtg gggccggacc gcgccgaagc
     4381 cgctgcccgc catctcttcg aagctgagcc tgttgttgcc gccttatcgc ttggtgttgc
     4441 aggcggactg agccctcagg tacagacagg ggacctgatt gtgggagacc gggtaatcct
     4501 tcgacgtgga tcaggacggg ccttattcgg ggaggaggac gcgaggccgg agaacttccc
     4561 gtgcgattcc agcttgcagg aggcagccct gaccgtcatc cgacgacttg gcagtcagta
     4621 ctatctcggc tcaattctca ccgtcgacag gattgttctg acgccaaagg agaagcgcct
     4681 actggcggcg gagtcgggcg ccatggccct ggacatggag agcgccgcca tcgcgtctgc
     4741 ggcctcggcg tattctattc ctttcctgac cattcgtggc gtcctcgatc cggtccacga
     4801 agatctcgcg atcgacttcg atcgatttct cgatgcagag ggggaaccaa gcctgccccg
     4861 attgatgcga tatctgatca cacatccgtt taccctgcct cgcttggtcg gactggggcg
     4921 tcggacgacg gcggtctgtg ctcgtctcgg tcgactgctg catgagctct caaccaccct
     4981 cagttgagcc gctgagggcc gtcatcgtag cgcgtttctc tggagatgtg tcttgggcaa
     5041 tcccgatctc tcaaaactga gtattgaccg gaccgcgact cgcgtatcgc acgcgcaacg
     5101 acggtggtgg ccctgggcgg tgggcctcgc cctgttggtc gctctcgcgg tactcggatt
     5161 ttccggcggc ctcggcggct ctgtgacggt ggagaccgcc tcggttacga ccgcgtatcc
     5221 gtcgcaggcg gtgaccatgc tgaacgctac cggctacgtc gttgcgcagc gcaaggccgc
     5281 agtggcgtca aaggcgacgg gacgcctcga gtggcttggc gtgatggaag gcagccgcgt
     5341 caagaaggac gaggtcgtcg cgcggcttga gagccgcgac gtcgcagcgg gccgcgagca
     5401 ggcggcggcg agcgtcaagg tcgccgaggc gaacctcgag caggcacagg ccgagctgcg
     5461 cgacgccgaa cgcaacttga cgcgctggcg cgagctgtcc cagaacaagc ttatcagcga
     5521 attggagctc gacaccgcga tagcacgggc cgacaaggcg cgcgcggcgg tgcggtcgag
     5581 tgaggccgcc atcgcggttg cgcgagcgaa cctgaaggcg gcggacgtct cgttcgacca
     5641 gacgttgatc cgcgcgccgt tcgacggggt agtgctcacc aagaacgcaa acgtcggcga
     5701 caatatcacc ccgttctcga gcgccgtcga tacgaagggc gcggtcgtga ccatagccga
     5761 tatgtcgacg ctcgaggtcg aggcggacgt ggcagagtcc tcgctcagtc ggatcaaggt
     5821 cggccaaccg tgtgagattc agctcgacgc cgtacccgac acacgctttg ccggcgtggt
     5881 gaaccgaatc gttccgaccg tcgaccgcgc gaaggcgacg gtgatggtaa agatcggatt
     5941 cgtagaccgc gacgagcgcc tactgcccga catgagcgcc aaggtcgcgt tcctcgagcg
     6001 cgaggtcgc
//
LOCUS       DATMXI010000221         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_57172, whole genome shotgun sequence.
ACCESSION   DATMXI010000221 DATMXI010000000
VERSION     DATMXI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57172"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..596
                     /gene="rfaE2"
                     /locus_tag="VN444_04125"
     CDS             90..596
                     /gene="rfaE2"
                     /locus_tag="VN444_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWR21031.1"
                     /translation="MTVGADKIRTLCELGAIVRQHRALGEQVVFTNGCFDLLHRGHTR
                     LLQQARALGDLLIVGLNSDASVRLLKGPFRPVLLQDERAELLSALASVDYVVIFEEPD
                     PRCVIEALEPDVLVKGADWAKEEVVGRETVERRGGRVVTIPLVEGSSTSSIMRRIMEM
                     APPQHVES"
     gene            820..>3907
                     /gene="mfd"
                     /locus_tag="VN444_04130"
     CDS             820..>3907
                     /gene="mfd"
                     /locus_tag="VN444_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWR21032.1"
                     /translation="MEQLEDLLIPEIAEIATRLDSGADALSLTGLFGASKALILSQVA
                     RLARRPLVVLASSSAEAELLAKDLQVFFRGPVGFLPERDEDPETGYQRIACLAGLATK
                     ELPLAVVSLQAALERLSPPSALLGAAFTLYVGRLIARDELLGVLDVGGYRRVNQVTDR
                     GEYSLRGHLLDLFPPKSVLSRIEESDLPVRAEFFGDEVLELRAFDPATQRSVRPIEQV
                     AVLPVVEVPLTGEACQQALEEPADLLQYVAPDTVWVLVDPAGLQAAARNFTEHGLRAS
                     HQIPPVPPLIKGGAGGFSFLDWSDFEQRLSLRPRLLLEEFLPTDSLAGEATIGFQVRT
                     ITAYRGRMMELIRDLEGWRRQSRRIHLVCRSEAQGRRLAEVLKEHDVAASLNPGMALP
                     SGITILTGELSGGFHLDEASLTYITESEIFGTRHIPPRRLRPKEVSPLASYQDLAYGD
                     FVVHEDHGIGLYKGLRQLAAGETEGDYLLIVYADHAKLYVPTSKLNLVYRYAGADGNS
                     PALDRLGSASWTKAKERVRASIREMAQELLTLYAARQVVKGYAFPPDTPWQREFEAGF
                     PYEETPGQLQAIADAKADMERDRPMDRLICGDVGYGKTEVAMRAAFKAVMGGKQVAVL
                     VPTTVLALQHFRTFSERFGNFPIKVEMLSRFRSRKEQGDVLRGVRDGIVDIVIGTHRL
                     LQKDVHFRDLGLLVVDEEHRFGVAAKERMKQLRRQVNVLTLTATPIPRTMHMSILGVR
                     DISTIETPPEDRLSIRTTVARFDQAIIKEAIEQELDRGGQVFFVHNRVESIHSVARLI
                     KRLVPRARLTVAHGKLPEERLERIMCDFYGGTFDVLLCTTIIESGLDVGAANTIIIDR
                     ADALGLAQLYQLRGRVGRDKHRAYAYLLVPEDTVLSEVAKKRLQAIAELTDLGSGFKV
                     AARDLEIRGAGNLLGPEQHGQIAAVGFDLYCRLIESTVRELKGEAAAESIEPSIRLEA
                     AGYVPEAYVEDPNVRFQLYKRLAALTSTQEVSAFREELIDRFGEPPQETERLLTAMV"
BASE COUNT          742 a         1127 c         1233 g          805 t
ORIGIN      
        1 acgcatgact tacccctaaa agccttcagc cttcagccta tctaccttgt agttatctcc
       61 cttttaccat ggaaggtcga ggaagcggaa tgacggtcgg cgccgataag atcaggacgc
      121 tctgcgaact gggtgccatc gtccggcagc accgggcgct gggagagcag gtggttttta
      181 ccaatgggtg tttcgatctg ctccatcggg gacatacccg cttgttgcag caagcgaggg
      241 cgctgggtga cctgctgatc gtcggcctga acagtgatgc atcggttcgg cttctcaagg
      301 gtccattccg gcctgtactc ttgcaggatg agcgggcaga gctgctctcg gccctggcct
      361 cggtcgatta cgtggtgatc ttcgaggagc cggacccgcg ctgcgtcatc gaagcgcttg
      421 agcccgatgt gttggttaag ggcgccgact gggcgaagga agaggtcgtt ggccgggaga
      481 cggtagagag acgtggtggg cgggtcgtca cgatcccgtt ggtggagggc tcttccacga
      541 gtagcatcat gcggcgcatc atggagatgg cgcctccgca acacgttgaa agctaagggc
      601 tgagtaacta cccaggtaga tagactgtag gctgttagac tgtaggaacg cgcacagatg
      661 agccatgcgt tttccgaatg cgacgcgaca atgatggaga ccaaaaagat gctgggcgcc
      721 ttgattcgat ccgtgcgcaa cgactcacag ctttcagcct acagcctaaa tatctgagta
      781 gttacatggt tgagagctga ccttatggtg atacgatcaa tggagcagtt agaggatctc
      841 ctgatccctg agattgctga gatcgcgact cgacttgaca gcggggccga cgcgctgtct
      901 ctgaccggtc tcttcggggc ttcgaaggcg ttgatcctct cacaggtcgc tcgcctggcg
      961 aggcgtcccc tcgtggtatt ggcctcctcg tccgccgagg ccgagctgct ggcgaaggat
     1021 ctgcaagtct tcttccgcgg cccggtgggc ttcctgccag aacgtgatga ggatcccgag
     1081 accggatatc agcgaattgc ctgcctggcg ggtctcgcca ccaaggagct tcccttggcg
     1141 gttgtctctc tccaggctgc cctcgaacgc ctctctcccc catcggccct cctgggagca
     1201 gccttcacgc tgtacgttgg acgactgatt gcgcgggatg agcttctagg cgtcctggat
     1261 gtaggtggtt accgtcgagt gaatcaggtg acagatcgcg gcgaatacag tctgcgcggg
     1321 catcttctcg acctcttccc ccccaaatct gttctgagcc gtatcgagga gtcggatctt
     1381 cccgttcgcg ctgagttttt tggcgacgag gtgcttgagc tccgcgcatt cgatccggcc
     1441 acccagcggt ccgtccgccc tatcgagcag gtcgccgtcc tgcctgttgt agaagtgcca
     1501 ttgacgggtg aagcgtgtca gcaggctctg gaggaaccag ccgatctgct gcagtacgtc
     1561 gcgcccgata ccgtgtgggt cctggtagat ccggcaggcc tccaggccgc tgcgcgcaat
     1621 tttactgaac acggccttcg cgcctcacac caaatccccc cagtcccccc tttgataaag
     1681 gggggtgcgg ggggtttttc attcctcgac tggagcgact ttgagcagag actctccctg
     1741 aggccgcggc tcctcctgga ggagttctta ccaacggaca gccttgcggg agaggcgact
     1801 atcggtttcc aggtgcgcac cattacggcc taccgtggac gaatgatgga gctcatacgc
     1861 gacctcgaag ggtggcgtcg acagagccgg agaatccacc tggtctgcag gagcgaggcc
     1921 cagggccggc gcctggctga ggtgctcaag gaacatgacg tcgcagcctc tctcaaccca
     1981 gggatggcgt tgccaagcgg gatcacgatc ctcactggcg aactcagcgg cggcttccac
     2041 ctggatgagg cttcactgac ctatatcacc gagtctgaga tctttgggac tcgacacatt
     2101 ccgccccgcc gcttacgacc gaaggaagtc tcccctctcg cctcgtatca ggacctggcc
     2161 tatggcgatt tcgttgtcca cgaggatcac gggatcggcc tgtacaaagg gcttcggcag
     2221 ctcgccgccg gcgagacaga gggtgactac ctgctcatcg tctatgccga tcacgctaag
     2281 ctctatgtac caaccagcaa gctcaatctg gtctatcgat atgcgggcgc tgatggcaat
     2341 tcacccgccc tcgaccggtt ggggagcgct tcctggacca aggccaagga gcgggtcagg
     2401 gcttccatcc gcgagatggc ccaggagctg ctcacgctgt atgcggccag gcaggttgtc
     2461 aagggctacg ccttccctcc tgatacgcct tggcagcggg aattcgaagc tggattccca
     2521 tacgaggaaa ccccgggcca gctccaggcg atcgccgatg ccaaggccga catggagcgg
     2581 gaccggccga tggaccgcct gatctgcggt gatgtcgggt atggcaagac cgaggtggcg
     2641 atgcgggctg cttttaaggc ggtcatgggg gggaagcagg tggcggtcct ggtcccgacc
     2701 actgtcctcg ccctgcagca tttccggacc ttttccgagc ggttcggcaa ctttccgatc
     2761 aaggtggaga tgctctcgcg tttccgcagt cgtaaggagc agggcgacgt gctgcgtggc
     2821 gtccgtgacg gcatcgtaga tatcgtcatc ggtacccacc gcctgcttca gaaggatgtc
     2881 cattttcggg accttgggtt gcttgtggtg gatgaggagc accgtttcgg cgtcgcagcc
     2941 aaggagcgca tgaagcagct ccggcgacag gtcaatgtct tgacgcttac cgcaacgccg
     3001 atcccgagga cgatgcacat gtcgatactg ggcgtcaggg acatcagcac gatcgagacc
     3061 cctccggagg atcgcctctc gatcaggacg accgtagcca ggttcgatca ggccatcatc
     3121 aaggaggcga tagagcagga gctggacaga gggggacagg tcttcttcgt tcataatcgc
     3181 gtagagagca ttcatagcgt ggcacgcctg atcaagcggt tggtcccccg ggcgaggctg
     3241 acggtggctc acggcaagct gccggaagag cgcctggaac ggattatgtg cgacttctac
     3301 ggcggtacgt tcgatgtcct gctgtgcact accatcatcg aatccggtct ggatgtcggc
     3361 gccgccaaca ccatcatcat cgatcgagcc gatgcgttgg gcctcgcgca gctctaccag
     3421 cttcgcgggc gggtcggtcg ggacaagcat cgagcgtacg cctatctctt ggtgccggag
     3481 gatactgtgc tctcagaggt ggcaaagaag aggctccagg ccatcgcgga actgaccgat
     3541 ctcggctctg gattcaaggt ggcggcaagg gacctggaga tcagaggagc gggcaacctc
     3601 ctgggacccg agcagcacgg tcagatcgcc gccgtgggat ttgatctgta ctgtagactg
     3661 atcgagtcaa cggtcaggga actgaagggt gaagcggctg cggagtctat cgagccttcc
     3721 atcaggctgg aggcggcagg gtatgtgccg gaggcctacg tggaggatcc caacgtccgg
     3781 ttccagctct ataaacggtt ggctgcgctc acatctacac aggaggtctc cgcttttcga
     3841 gaggaactga tcgatcgctt cggtgagcct ccccaagaga cagagcggct actgaccgca
     3901 atggtcc
//
LOCUS       DATMXI010000222         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_285066, whole genome shotgun sequence.
ACCESSION   DATMXI010000222 DATMXI010000000
VERSION     DATMXI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285066"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="gap"
                     /locus_tag="VN444_04135"
     CDS             complement(<1..960)
                     /gene="gap"
                     /locus_tag="VN444_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWR21033.1"
                     /translation="MAIKVAINGFGRIGRNVFRAALGDRELEFVAVNDITNAKTLAHL
                     LKYDSIHGVLQADVQAKDNAIVVNGREVKICAQRDPAALPWKELGVQVVVESTGRFTD
                     KAGASKHLQAGAKKVIISAPAKDPDVTIVLGVNEKMYDPDKHAIISNASCTTNCLAPI
                     AKVIMENFGIRHGLMTTIHSYTNDQQILDLPHEDLRRARAAALSQIPTSTGAAKAVGL
                     VLPALQGKMHGLAIRVPTANVSLVDLVAETERVVTVEEVNAALRKAADGELKGILGFC
                     EEPLVSVDFCGNSLSSIVDSLSTSVVDGTLIKVLSWYDNEWGYS"
     gene            complement(1294..1752)
                     /locus_tag="VN444_04140"
     CDS             complement(1294..1752)
                     /locus_tag="VN444_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWR21034.1"
                     /translation="MRQAILLFKYGGRRTLARHLGRLMVEGAGRLFDPRQFDFLIPVP
                     LHPKRERARGFNQAALLAKEIGVGWDLRVGHRVLQRVRATEAQSGGRREREENVKGAF
                     AVLRPERVKGMRLLLIDDVFTTGATAGECAKVLLAAGVAEVGVYTLARVA"
     gene            complement(2028..2381)
                     /locus_tag="VN444_04145"
     CDS             complement(2028..2381)
                     /locus_tag="VN444_04145"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWR21035.1"
                     /translation="MPLRAALLERLKEKLLEKRRALMNTVREKQADNLETGSDGTQDI
                     ADQATTAYTKEFLLSISDTERQLLKQVDAALEKMRLKKYGECERCGEPIGEKRLEALA
                     FALFCIACQEEEERS"
     gene            complement(2560..2952)
                     /gene="rsfS"
                     /locus_tag="VN444_04150"
     CDS             complement(2560..2952)
                     /gene="rsfS"
                     /locus_tag="VN444_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019542740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWR21036.1"
                     /translation="MSRDARERIDTDTLLRLAAAAAEEIKPISMVHLDLRSLCSFTDH
                     FLIVSAPSIRQVRAIAERIEERLREEGVQIFHREDDKEARWILLDYSDVIIHIFDEEM
                     RLYYDLEGLWADAPKQELVRRGSSGLSG"
     gene            complement(2973..>3385)
                     /locus_tag="VN444_04155"
     CDS             complement(2973..>3385)
                     /locus_tag="VN444_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21037.1"
                     /translation="FIMGADAFLDIATWKEAQELLSLAQVIVTARPGWRLNEVERSMT
                     SEHRQLLGNPRLKYVKISDVTRETAKVHHEPRSVLLVEVVSLDISSSEIRQLVKEGRS
                     IRYLVTDTVAAYIDKNRLYHPGRKGPIGEREAST"
BASE COUNT          742 a         1040 c          892 g          711 t
ORIGIN      
        1 ggagtagccc cactcgttgt cgtaccagga caggaccttg atcagggtac cgtctacaac
       61 cgatgtcgaa aggctatcaa caatggacga aagcgaattg ccgcaaaaat cgacagagac
      121 caacggctcc tcacagaaac cgaggatccc tttcaattca ccatcggctg ccttccgaag
      181 ggctgcattc acctcctcga ccgtgaccac ccgttccgtc tccgcaacga gatcgaccaa
      241 agacacgttt gccgtcggga cgcggatagc aagcccgtgc atctttccct gtagcgcagg
      301 gagcaccagg cccaccgcct tggccgctcc agtgctggtg gggatctggg aaagggcagc
      361 agccctcgcc cggcggaggt cctcatgggg aaggtccagg atctgctgat cattcgtata
      421 ggaatggatc gtggtcataa ggccatggcg aattccgaag ttctccatga tcaccttcgc
      481 gataggcgca aggcagttgg tggtacagga tgcattgctg atgatcgcgt gcttgtccgg
      541 atcgtacatc ttctcgttaa cgccaagcac gatcgtaaca tccggatcct tcgccggtgc
      601 tgaaatgatg accttcttcg ccccagcctg caggtgcttg ctcgccccag ccttgtcggt
      661 aaagcgtccg gtcgattcga cgactacctg tacccccagc tctttccagg gaagcgccgc
      721 cgggtctctc tgggcacaga tcttaacctc gcgaccattg accacgatgg cattatcctt
      781 agcctgcaca tccgcttgta gcaccccatg gatagaatcg tacttcaaca ggtgggcgag
      841 cgtcttggcg ttggtaatgt cgttcaccgc gacgaactcc aactctcggt ctccgagcgc
      901 tgcccgaaag acattccggc cgatgcggcc gaatccgtta atcgcaacct tgatcgccat
      961 gcctcgcttc tcctccttga agtaacgaac cgccgactgt tgttccggtg gacgaacaaa
     1021 caaccagaac gtattccgct agaaggcaaa tcagcccttt tttataaggc ctttgatctt
     1081 aaatgtcaac cccttaaggt caattccaca tagaattcgt aacgactcag gtgtttaggc
     1141 tgaaggctga aggcttgtag gggtaaggtg tacatgatca tacaaaactc cgaaaaggtc
     1201 ttgaatggct cgatacgtgc cttgcgagat gattgagaac cttcagcctt cagtctaaac
     1261 acctaagtag ttagcggagt tccacgggca gattcacgcc acccgggcga gtgtgtaaac
     1321 tccgacttcc gcgacaccag cagcgagcag aacctttgca cactcgccgg ctgtggcgcc
     1381 ggtggtaaag acatcgtcaa taaggaggag tctcatgccc ttcacccgct caggccgcag
     1441 aacagcaaag gcccccttga cgttttcctc tcgctctcgc cgacccccgc tctgagcctc
     1501 cgtggcccgg acccgttgta atacacggtg accgacgcga agatcccagc caaccccaat
     1561 ctccttcgcc aagagcgccg cctggttgaa acctcgcgca cgctcccgct tgggatggag
     1621 tggaaccgga atcaggaaat cgaactggcg gggatcgaag agtcgtccgg ctccctcgac
     1681 catcagacga ccgaggtggc gagcaagcgt gcgacggccg ccgtacttga acagcagaat
     1741 ggcctgacgc atcgtccggt ccgcttcgta caacgctgcc gccctcgcca tagcaaaggg
     1801 tggaagctgc cctcggcagg gtccgcacag gtggcctggg ctttcctcaa gggttctcgg
     1861 agaggcgaat ggcctgccgc agcggggaca gaacggttcg gaaacgagcc gaatctcaag
     1921 ccagcagcgg ccgcagatga cggaccggcg ggtagcgtcc aggggttgat gacacactcg
     1981 gcagggggaa ggaaaaatga gatgaagaaa ggctgccagc agctccttca gctccgctcc
     2041 tcttcctcct ggcaggcaat acagaatagg gcaaaggcca acgcctcgag ccgcttctcg
     2101 ccgattggct cgccgcaccg ttcgcactcc ccgtacttct tcagccgcat cttctccagg
     2161 gcagcgtcca cctgcttcaa caactggcgc tcagtatcgc tgatagagag caaaaattcc
     2221 ttcgtgtagg ctgtcgtggc ttgatcggcg atatcctgcg taccgtcgct accggtctcc
     2281 agattgtccg cctgcttctc ccgcactgtg ttcatcaacg cacgtcgctt ctctaacagc
     2341 ttttccttta atcgttccag cagtgctgcc ctcaacggca tccccatcac ctctctgcct
     2401 gtgtgtggcc gcacgcaggc aagccacccc agcgattagg aagatcaccc tcaccctcaa
     2461 aagggagagt ggaggctgca gccacggcta atccccaata aaaaaagaag cgaggctcat
     2521 gagcccccgc ctcccggata tcgacttcat tatggcggtc tatccagaaa gacccgacga
     2581 tccgcgacga actaactcct gtttcggcgc gtcggcccac aacccctcca gatcatagta
     2641 cagccgcatc tcttcatcga aaatatgaat gatcacgtcg ctgtagtcaa gaagaatcca
     2701 acgggcctcc ttgtcgtcct cccgatggaa tatctgaact ccttcttccc gaagccgctc
     2761 ctcgatccgt tcggcaatgg cccggacctg tctgatcgag ggggcactca cgatgaggaa
     2821 atggtcggtg aatgagcaga ggctacgaag atccaggtgt accatggaaa taggtttaat
     2881 ctcttctgcc gctgctgctg caagtcgcaa cagcgtgtcg gtgtcgatcc gctctctcgc
     2941 gtctcgtgac aatacaggtc gtttgtctga actcacgtcg aagcctcgcg ttcgcctatt
     3001 ggcccttttc gcccaggatg atacaggcgg ttcttgtcta tgtaagcggc tacagtgtcg
     3061 gtcaccagat accggatact cctcccctcc ttcacgagtt gccggatctc gctggaggag
     3121 atatccagcg agaccacttc aaccaacaga accgatcggg gttcatggtg tacttttgcg
     3181 gtctctctcg tgacgtcaga gatcttcacg tacttcaatc gggggttacc caggagctgc
     3241 cgatgctcgg acgtcatgga gcgctccacc tcattaagcc gccagccggg ccgggcagtg
     3301 acgataacct gggcaagtga cagcaactcc tgtgcttctt tccaggtggc gatatcaagg
     3361 aacgcatctg cccccatgat gaagt
//
LOCUS       DATMXI010000223         9551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_132937, whole genome shotgun sequence.
ACCESSION   DATMXI010000223 DATMXI010000000
VERSION     DATMXI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132937"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1150)
                     /gene="mqnE"
                     /locus_tag="VN444_04160"
     CDS             complement(5..1150)
                     /gene="mqnE"
                     /locus_tag="VN444_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HWR21038.1"
                     /translation="MESRLAASGLDKVYQKILQGIRLDFCDGLALYQTRDLTSVAFLA
                     NLVRERLNGDLTYFVRNLHINYTNICNKGCKFCSFYAPPSDPRGYVLSIQDIQDQIRK
                     HDEVPIREIHIVAGINPKLPYQYYLDLVRAVKDVRPGIQVKAFTMIELAQIQRVANKP
                     MEEVLADLKAAGLDCCPGGGAEVFSDRVHEELFRAKLDNDEWFDVARAVHRAGIRSNA
                     TMLYGHIETVEEKVTHLLHIRDLQDETGGLMCFIPLAFDPANTELSHLPITTGYANLR
                     EIAIARLLLDNVPHIKAFWIMTTPAVAQIALWYGADDLDGTVTHYEITHALGDTSHHQ
                     ELAHAELLAMIHEAGRHPVERDALYNPLHSHSSGRLEGSRAGRVSVA"
     gene            complement(1159..1479)
                     /locus_tag="VN444_04165"
     CDS             complement(1159..1479)
                     /locus_tag="VN444_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21039.1"
                     /translation="MEILIITLLVFLALLPVIIPFFRSSEGSPLGSEQTELEDLLAEK
                     QMVYAAIKELEFDHQAGKLSLEDYEQARHSYELRAIALLKQIDGMGAQQGSGDVKSRE
                     KPSR"
     gene            complement(1484..1933)
                     /locus_tag="VN444_04170"
     CDS             complement(1484..1933)
                     /locus_tag="VN444_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWR21040.1"
                     /translation="MCLLLTIALGVWPSVILAGSIEEQTHLLAAELRCPVCQNLSVAD
                     SPSEMAIQMREVIFEKLKNGESPEQIRGYFVSRYGEWILLAPTRKGFNWIAWLLPFVV
                     ILGGAGIIILRIRRAIGRGHGPNADASHPLDPSYTSQLEAELKESER"
     gene            complement(2050..2568)
                     /locus_tag="VN444_04175"
     CDS             complement(2050..2568)
                     /locus_tag="VN444_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWR21041.1"
                     /translation="MATWKKLSLLLSIIPLLLLLAYGFKTNPRDVPSPLVGHQAPPFA
                     LSMFDGSSNSLEQLRGKVVILNFWASWCFPACYEEAPHLEAAWQTYRDRGVMVIGVDM
                     QDRDTEAKAFIERLKLSFPNGPDPQGKISINYGIYGVPETFFIRKDGTIHYKHVGALD
                     AKLLKAKIEEML"
     gene            complement(2547..4547)
                     /locus_tag="VN444_04180"
     CDS             complement(2547..4547)
                     /locus_tag="VN444_04180"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWR21042.1"
                     /translation="MIATLGRFSLWLAFAIAIYGAFVSLLGVRRRRPALIESGQGAVF
                     AIFGLTTFAVLLMEAGLVGRDFSLEYVARNASLDTPLFYTIIALWGALEGSILLWVWL
                     LALMTLLTVMIDGKRQPELIPYVTAVLLCISAFFLLLVTFPASPFTTMLPVPLDGRGP
                     NPLLQNHPLMAIHPPGLYAGYVGWSIPYAFGIAALITGRLGEGWIRTVRRWSIVSWAF
                     LTVGIIVGGRWSYEVLGWGGYWAWDPVENASLLPWLTGTAFLHSIMIQERRKMLKNWN
                     VALVIITFALTIFGTFLTRSGILSSVHAFSDSTVGPLFLVFIGLILFFSFGLLVYRSG
                     KLQAESDIDSLVSRESAFLANNLLLLGFAFAVLLGTLFPLLSEAVRGVKVSVGEPFFN
                     AVNVPIGLGLIFLMAIGPLIAWRHASLDSLRRTFIRPLCLTILGAGICWFLGVRGLYP
                     LLAFALSLFTAITVIQEFAGGAMTRRRNTGEGYLIALSKLTSRGRRRYGGLIVHLGVA
                     LVVVGITASSVFKLEQEVTLKKGESLQLGRYQVRFDELSAWQEPHRFVVQGSFTVFNS
                     NHKVAEMRPAKRFYPAEQQPIGTVDVRSTAREDIYLVLSSFAQDGTTATVKALVRPLV
                     MWIWFGGWVMVLGSLIAIWPDRRRALATDQVEERMAWQPGRS"
     gene            complement(4544..4993)
                     /locus_tag="VN444_04185"
     CDS             complement(4544..4993)
                     /locus_tag="VN444_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWR21043.1"
                     /translation="MTKKTKLLLGAVVIASTIGYLIVGGIKEAAVYYITPTELKAKGP
                     ATTDRAFRLGGMVVAGSRRWDPQTLKLAFSLSDDKEQVAVEYVGSPPDLFKEGAGAIV
                     EGKYMNGLFRADTILAKHSEEYRPPEEGQATAKDHYRTLVKPPAARP"
     gene            complement(4990..5136)
                     /locus_tag="VN444_04190"
     CDS             complement(4990..5136)
                     /locus_tag="VN444_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21044.1"
                     /translation="MKPWSYNLAAYLLSAVGLLWYLVSVGRRMKAVRAQIMALKHQWK
                     DMAN"
     gene            complement(5133..5816)
                     /gene="ccsA"
                     /locus_tag="VN444_04195"
     CDS             complement(5133..5816)
                     /gene="ccsA"
                     /locus_tag="VN444_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWR21045.1"
                     /translation="MTLQRIEQVLGVCTALGLLIGLYIGLIYAPPDAVQGDVQRLMYL
                     HVPLILVSYLAFFVVFVASILYLWRRRRQYDAIAHSSAEIGTLFTALAIATGSIWGRP
                     TWGAWWTWDARLTSTAILLLIFLGYLMLRALMEDESRGAVFCAVLGIIGFLDIPIIHM
                     SVVWWRTLHQPASILKSGLWTVAPDMLMALLTNLAAFVLLYGYLLAKRLRLEGAKEEL
                     FNLRMELLG"
     gene            complement(5821..6498)
                     /locus_tag="VN444_04200"
     CDS             complement(5821..6498)
                     /locus_tag="VN444_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWR21046.1"
                     /translation="MTFARRVLAIAWKDLVAEWRDRESITAMCFFAFLVLFLFSFALN
                     GDQALIRGASSGLLWLAFAFTGVLGLARSVQSELANDCLDGLLLYPAEREAIYLGKLL
                     GNFSVILLVELISFPIFAILYNMDLWPQLPKLLLITVPATLGFAAAGTLLSTMTAGLR
                     AREAMLPFLLFPMTIPLILAAVRGTEVVLRHESFELAMPWLKLMAAFDLLFLVGSLLT
                     FEFLVEE"
     gene            complement(6495..7247)
                     /gene="ccmA"
                     /locus_tag="VN444_04205"
     CDS             complement(6495..7247)
                     /gene="ccmA"
                     /locus_tag="VN444_04205"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWR21047.1"
                     /translation="MRTDGAAGLHGVAPAVETRGLVKWFGAHPALRGINLSVAKGEIL
                     ALFGPNGAGKSTLLRILAGLMRPTAGSAQVAGLDVGRDGDSVRRVIGVLASGHQLYEA
                     LTGRENLLFAATMLGLDRHAERVAKVLEKVGLEKAAEGRVRTFSSGMKRRLALAKLML
                     HEPKIMLLDEPFTNLDLQATKLLEEFLMTSKITGATTVLATHNLAIGFALADRMAILR
                     QGRLVFDARRDDVSLESLRSQFALHGELWEVE"
     gene            complement(7550..8557)
                     /locus_tag="VN444_04210"
     CDS             complement(7550..8557)
                     /locus_tag="VN444_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWR21048.1"
                     /translation="MDPERPPLQTILVVDDQEVNIVLVRAILAPQGYTILAASNGEQA
                     IELVAAQPPDLILLDIMMPGENGFDVCARLKEDERTRLVPIVMLTSLNSPKDKIRGIE
                     VGADDFLSKPFHSAELSARVRSLLKLKQFTDELENAEDVLFTLALSVEAKDAYTDGHC
                     ERLSLYSVALGRRLGLTREQLTALHRGGYLHDVGKIAVPESILNKKTGLTDEERRSMR
                     EHPIIGERICRPLKSLKLVLPIIRHHHERWDGSGYPDGLKGQEIPLSARIIQTVDIYD
                     ALTTARPYKPSLETCEVFSIMRQASEKGSCDPRLIEQFISLLQSGETLMGLEKVRHRR
                     L"
     gene            complement(8557..9288)
                     /gene="lipB"
                     /locus_tag="VN444_04215"
     CDS             complement(8557..9288)
                     /gene="lipB"
                     /locus_tag="VN444_04215"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWR21049.1"
                     /translation="MPICSLIDLGLVPYDESLMLQRRLATLRVDDRLGDVLLLVEHPP
                     VITLGRAGRMAHLRVPEPSLTALGIEFFEVERGGDMTYHGPGQLVGYPILNLVEHGRD
                     LHRYLRQLEEVLIMTLSGFGITAGRSLGRTGVWVGDEKIASLGIHVSRWVTRHGFALN
                     VNMDLAPFELIVPCGIQGARTTSMTQELSRPISVREVMAALIERFEVVFGASLLSTSL
                     PELFRLRSQAGRIERGGPAIVRGAS"
     gene            complement(9424..>9551)
                     /locus_tag="VN444_04220"
     CDS             complement(9424..>9551)
                     /locus_tag="VN444_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21050.1"
                     /translation="GMAIALEATPEEIARSIHAHPTLSEAMGEAALAALGRAVHV"
BASE COUNT         2093 a         2941 c         2700 g         1817 t
ORIGIN      
        1 cgggttacgc tacgctaacc cgacctgctc gactcccctc gaggcgacca gaagaatggg
       61 agtggagggg gttgtagagc gcgtcgcgct cgactgggtg gcggcctgcc tcgtggatca
      121 tggcgagaag ttcggcgtga gcaagctctt gatgatggga ggtgtcgccc agggcgtggg
      181 taatctcata gtgggtgacc gtcccatcga ggtcatccgc cccgtaccag agggcgatct
      241 gcgccaccgc cggtgtggtc atgatccaga acgccttgat gtgcgggaca ttgtccagca
      301 ggagcctcgc aatcgcgatc tcacggaggt tggcataccc tgtcgtgatc gggagatggc
      361 tgagctcggt attggccgga tcgaacgcca gcgggataaa gcacatcaag ccgcccgttt
      421 cgtcctgcag gtcgcggata tgcagcaggt gcgtgacctt ttcttccacc gtttcgatat
      481 ggccatagag catcgtggcg tttgaacgga taccggcccg atgtacggcc ctggccacgt
      541 cgaaccattc gtcgttatcc agtttggccc ggaacagctc ttcgtggacg cggtcactga
      601 acacctctgc cccgccgcca gggcaacaat cgagccccgc cgccttcagg tcggccaaga
      661 cctcttccat cggcttgttg gccactcgct ggatctgcgc cagttcgatc atggtgaacg
      721 ccttgacctg gatgcccggc ctgacatcct tcactgccct aacgaggtcg aggtagtact
      781 ggtacgggag cttcggattg atcccggcca ctatgtggat ctcccggatg ggaacctcgt
      841 cgtgcttgcg gatctgatcc tggatatcct ggatgctcag cacataccct cgtgggtcac
      901 tgggcggggc ataaaacgag caaaacttgc accccttgtt gcagatgttc gtgtagttga
      961 tgtgcaggtt gcgaacgaaa taggtgaggt cgccgttcag gcgctcgcgc accaggttgg
     1021 cgagaaaggc caccgaggtc aagtcgcgcg tctgatagag cgccagtccg tcacagaagt
     1081 ccaggcggat gccctgcagt atcttctgat acaccttgtc caggcccgag gcagcgagcc
     1141 gagactccat gaggcggctc atcggctcgg cttctctctg gactttacgt ctccgcttcc
     1201 ctgttgcgca cccatgccgt cgatctgttt gaggagggca atcgctctca gttcatagct
     1261 atggcgcgcc tgctcatagt cttcgaggct cagcttccca gcctggtgat cgaactcaag
     1321 ctccttaatt gcggcgtaaa ccatctgttt ctcggccaga agatcctcca gctcggtctg
     1381 ttctgacccc agtggagatc cctcagacga tctgaagaat ggaatgatca ccggcaagag
     1441 ggcgagaaac acaagcaagg tgataatcag gatttccacg tcgtcagcgt tccgattcct
     1501 tgagttccgc ttcgagctga ctggtgtagc tggggtcaag ggggtgcgat gcatccgcgt
     1561 ttggcccgtg acctctccca atcgcgcgac ggatcctgag aatgatgatc ccagctccgc
     1621 ccaggatgac gacgaatggc agcagccacg cgatccagtt aaaaccctta cgcgtcggcg
     1681 cgagcaaaat ccactcgcca tagcggctca cgaagtagcc tcgaatctgc tccgggcttt
     1741 caccgttctt gagtttctcg aagattacct cgcgcatctg gatcgccatc tctgagggcg
     1801 agtcggctac cgagaggttc tgacacaccg ggcagcgcaa ctcggcagcc agtaggtggg
     1861 tctgctcctc gatgctgccg gcaagtatga cggatggcca taccccaagc gcaatcgtca
     1921 gaagcaggca cacgacccag cgccctcttc tgccatccga attctgtgtc aaattctccg
     1981 tacctcgcat ttcgcacccc gcaccaaatc ccccctcgcc cccctttgct aaagggaggt
     2041 tggggggatt tagagcatct cctcgatctt tgccttcagc agcttcgcgt cgagggctcc
     2101 gacgtgcttg taatgaatcg taccatcctt cctgatgaag aaggtctccg gcacgccata
     2161 gatgccgtag ttaatggaga tctttccctg cgggtcagga ccgtttggaa agctgagttt
     2221 gagccgctcg atgaacgcct ttgcctcagt atcccggtcc tgcatatcca ccccgatgac
     2281 catcacccct cgatcccggt aggtctgcca ggcggcctcc aggtgcggcg cttcttcgta
     2341 gcaggccggg aagcaccagg aggcccaaaa gttcaggatc acgaccttcc cgcggagctg
     2401 ctccaggctg ttactgctcc catcaaacat ggaaagagcg aacgggggcg cctggtgtcc
     2461 caccagcggc gatggtacat ccctcgggtt ggtcttgaag ccataggcca ggagtagcag
     2521 gagcgggata atactgagca gcaggcttag cttcttccag gttgccatgc catgcgctcc
     2581 tctacctggt ctgtagcgag ggcccgtctc cggtcgggcc agattgcgat cagcgaaccc
     2641 aggaccatca cccagccacc aaaccagatc cacatcacca gcgggcgaac cagcgccttc
     2701 acggtggcgg tagttccatc ctgtgcaaac gacgaaagga ccaggtagat atcctcccgt
     2761 gccgtcgagc ggacatcgac ggtgccgata ggctgctgct ctgccgggta gaaccgcttg
     2821 gcaggtcgca tctcggctac cttatggttc gagttgaaga ccgtaaagct cccctgcacc
     2881 acaaagcgat gcggctcctg ccaggcgctg agttcgtcaa atcgaacctg gtaccgacca
     2941 agctggagcg actcgccttt cttcagggtg acctcctgct ccagtttgaa gaccgacgag
     3001 gcggtaatgc cgaccacgac gagcgctacc cccagatgga cgatgaggcc gccatagcgc
     3061 cgtcggcccc ggctggtgag tttcgagagt gcgatcagat acccctctcc tgtgttccgg
     3121 cgtcgcgtca tggcgccacc ggcaaactcc tgaatgacag tgatcgcggt aaataggctg
     3181 agggcaaagg ccaggagcgg atataatcca cgcaccccaa ggaaccagca gatgcccgca
     3241 cccaggatgg tgaggcacaa gggtctgata aaggtccggc gcaggctatc gagcgaggcg
     3301 tgtcgccagg cgatcagcgg cccgatagcc atgaggaaga tcaggcccag gccgatgggg
     3361 acgttcaccg cgttgaagaa cggctcgccc acgctgacct ttaccccgcg aactgcctcg
     3421 gacaggaggg gaaacagcgt gcctagcaga accgcaaacg cgaatcccag cagcagcaga
     3481 ttgttcgcca ggaaggcgct ctcgcgggac accagcgaat cgatatcact ctccgcctgt
     3541 aacttgccgc tccggtacac caggagaccg aaggagaaaa acaggatcag gcctatgaag
     3601 accagaaaca acggcccgac ggtcgagtca gagaaggcgt gaacggacga aaggatgccg
     3661 cttcgcgtga gaaaggtgcc gaagatggtc agcgcgaagg tgataatgac cagcgccacg
     3721 ttccagttct tcagcatctt tcgtcgttcc tggatcataa tcgagtggag aaatgccgtt
     3781 ccggtaagcc agggcagcag cgaggcgttc tccaccgggt cccaggccca gtagccgccc
     3841 caccccaaca cctcgtagga ccaccgccct ccgacgatga tgccgaccgt caggaaggcc
     3901 caggatacga tcgaccagcg acgtacggtt ctgatccatc cctcgccgag ccgtccggtg
     3961 atcagggcgg ctattccgaa ggcatacgga atggaccatc cgacataacc ggcgtacaag
     4021 cccggggggt gaatcgccat caggggatga ttttgcagga gcggattcgg cccgcgtccg
     4081 tcaagtggca ccgggagcat cgtcgtaaag ggactggccg gaaaggtcac caggaggagg
     4141 aaaaacgccg aaatgcacag caggacagcc gtcacgtacg gaatcaattc gggctgccgc
     4201 ttcccatcga tcatcaccgt aaggagggtc atcagggcca gtagccagac ccagagcagg
     4261 attgagccct cgagcgctcc ccacagtgcg atgatcgtgt agaacagcgg tgtatcaagg
     4321 ctggcattcc gggccacgta ctcaaggcta aaatctcggc caaccagccc agcctccatc
     4381 agcagaactg cgaaggtggt gaggccaaag atggcgaaga ccgccccttg accgctctca
     4441 atcagggccg gccgacgtct ccgtactccc aggagggaga cgaacgcgcc gtagatcgcg
     4501 atggcgaagg ccaaccaaag actgaaccgg ccgagcgtcg caatcatggt cgcgcagccg
     4561 gcggcttcac cagtgttcgg tagtgatcct tcgccgtggc ctgaccctcc tcaggcggcc
     4621 ggtactcctc agaatgcttg gccaggatcg tatccgcccg aaagagaccg ttcatatact
     4681 tcccctccac aatcgcaccg gctccctcct tgaacaggtc aggtggcgag cccacgtact
     4741 cgaccgctac ctgctctttg tcatcgctca agctgaaggc cagcttcaga gtctgcggat
     4801 cccagcggcg cgatccagcc accaccatcc cgcccaggcg gaaagctcta tccgtcgtcg
     4861 ctggtccctt tgccttcagt tccgtcggtg tgatgtagta gacggcggcc tctttgattc
     4921 cgccaacgat cagatagccg atggtcgacg cgatgacgac cgcccccaga agcaatttag
     4981 tctttttcgt caattggcca tgtccttcca ctggtgcttg agggccatga tctgcgccct
     5041 cactgccttc atacgcctcc cgaccgagac gagataccag agcaggccca cggcggacag
     5101 cagatacgcc gccagattat aggaccacgg cttcacccga gtagctccat cctcaggtta
     5161 aacagctctt ccttcgcccc ctcgagccga agtcgcttgg ccagaagata tccgtacaac
     5221 agcacaaagg cagcgaggtt cgtcaggagc gccatcagca tgtccggagc caccgtccag
     5281 agaccagatt tcaggatcga cgcgggttgg tgcagggtgc gccaccacac gacggacatg
     5341 tggatgatcg gaatatcgag aaagccgata atcccaagga cggcgcagaa gacggcccct
     5401 cgcgactcat cctccatcag cgcccgaagc atcagatagc cgaggaagat gagcaacagg
     5461 atagcggtcg aggtcagtcg cgcgtcccac gtccaccagg ccccccacgt cggtcgcccc
     5521 catattgagc cggttgcgat cgccagggcg gtgaagagcg tgccgatctc ggcggacgaa
     5581 tgcgcgatcg catcgtactg ccggcgacga cgccacagat acaggatgct cgccacgaag
     5641 acgacaaaaa aggccaggta gctgacgagg atcaagggga cgtgcaggta catcaaccgc
     5701 tgtacgtctc cctgcaccgc atcgggcggc gcatagatca accctatgta caggccgatc
     5761 agtaatccaa gggcggtaca cacacccagc acctgttcga tccgttgcaa ggtcattgtc
     5821 tcattcctca accagaaact cgaatgtcaa cagcgacccc acaaggaata gcagatcaaa
     5881 cgccgccatc agcttgagcc agggcatggc aagttcgaat gactcgtgcc gcagaacgac
     5941 ttccgtcccc ctgacggcag ccaggatcaa ggggatcgtc atgggaaaca ggaggaacgg
     6001 cagcatggcc tcccgcgccc tcaggcccgc tgtcatggta gacaacaggg tgcctgccgc
     6061 ggcgaatccg agcgtcgccg gcaccgtgat taacaggagc ttgggcagtt gcggccagag
     6121 gtccatattg tacaggatcg caaaaatggg aaaacttatc agctctacca ggagaatgac
     6181 gctgaagttg cccaacagtt ttcccaggta aatcgcctcc cgctcggcag gatacaggag
     6241 caggccatct aaacagtcat tcgccagttc gctctgaact gatcgagcca gaccaagcac
     6301 gccggtgaat gcaaacgcca gccacaacag gccggatgag gctccccgga taagcgcctg
     6361 atccccgttg agcgcgaagc tgaagaggaa cagaaccagg aaggcgaaaa aacacatggc
     6421 ggtgatgctt tcccgatctc gccattctgc gaccagatcc ttccacgcga tcgccaggac
     6481 cctccgggca aaggtcattc gacctcccac aactccccat gaagcgcaaa ttgggagcgt
     6541 aacgattcca gactcacatc atcccgtcgg gcgtcaaaga ccaaccgccc ctgccgcaag
     6601 atagccatgc ggtccgccag ggcaaaccct attgccaggt tgtgggtggc caatacggtc
     6661 gttgcaccgg tgatttttga ggtcatgagg aattcctcca gcagcttggt cgcctggagg
     6721 tcaagattgg tgaatggctc gtcgagcagc ataatctttg gctcatggag catcagcttt
     6781 gccagggcaa ggcgccgctt catcccactg gagaaggtcc tgacccgacc ctctgccgct
     6841 ttctctagtc cgaccttctc cagaaccttt gctactcgtt cggcgtgacg atccagtccc
     6901 agcatggtcg ccgcgaagag aaggttttct cgcccggtca gagcctcata cagttggtga
     6961 ccgctggcaa gtacaccgat aacccgcctt acactatcgc catccctgcc cacatcgagc
     7021 ccggcgacct gcgccgatcc ggcagtgggt cgcatcagtc cggccaggat tctcagcagg
     7081 gtgcttttgc ccgctccgtt cgggccaaac agagccagga tctctccctt ggcaacggaa
     7141 aggttaatgc cacgcagggc cggatgcgcg ccgaaccact tgaccaaacc cctggtctca
     7201 acggctggcg caaccccatg gagccccgct gctccatcag tcctcaccgt tcgagactct
     7261 gagtatggga agctacggca ccatcaacgc ccccgtcaca taagacgcgg agccgcaact
     7321 gctctgccaa cgtcagcatt gccatcatct gcttgcagag aggagcgcta cagggagacg
     7381 ttctgacgga caatcacgca tcgttgacag ggcgcctgat cccaaccacg gattcacgcg
     7441 gatggcgaaa atggagtcga atacgcgttc gaatcctgtt ctcaagacag gcttagttta
     7501 gcagagatta tgtggaggaa caacctaaaa tggccgacag ggcaccaagc tacagccgcc
     7561 tgtgacgtac cttctccaag cccatcaggg tctcaccaga ctgcagtaag ctgataaact
     7621 gctctattaa cctggggtcg catgaaccct tctctgatgc ctgccgcatg atcgaaaaga
     7681 cctcgcaggt ctcgagtgag ggcttatagg ggcgggccgt cgtgagcgca tcgtagatat
     7741 caacggtctg aatgatcctg gccgagagag gaatctcctg tcccttcaag ccatcgggat
     7801 acccgcttcc gtcccatcgt tcatggtggt ggcgaatgat gggaagaacc aatttcagcg
     7861 acttgagcgg cctgcaaatc cgctccccga ttatcgggtg ttcccgcatg ctccgccttt
     7921 cttcatcggt caatcccgtc tttttattca ggatcgactc cggtacagca atcttgccga
     7981 cgtcatgcag gtagcctcca cggtgtagcg ccgtcaactg ctcacgggta aggcccaggc
     8041 gccggccaag ggcaaccgag taaagggata atcgctcgca gtgtccgtcg gtgtacgcat
     8101 ccttggcctc gacgctcagg gccaacgtaa ataggacgtc ctccgcattt tccagctcat
     8161 cggtaaactg cttgagcttc agcagggatc ggactcgcgc gctcaactct gccgagtgaa
     8221 aaggcttgct caaaaagtcg tctgccccaa cctcgatccc acggatttta tctttcggag
     8281 agttcaggga ggtcagcatc acgatgggca caagtcgggt tcgttcgtcc tccttgagcc
     8341 gggcgcagac gtcgaacccg ttctcgccag gcatcatgat gtcaagcagg ataagatccg
     8401 gaggctgggc tgccaccagt tcgatggcct gttccccatt cgatgccgcg agaatcgtat
     8461 aaccctgcgg ggccaggatg gcccgaacca acacgatgtt gacttcctga tcgtcgacga
     8521 ccaaaatcgt ctggaggggt gggcgttccg ggtccatcaa ctcgcgcctc gaacgatagc
     8581 tggtccgccc ctctcgattc tgcctgcctg gctcctcaaa cggaaaagtt caggcagcga
     8641 cgtagacaga agagacgccc cgaacacaac ctcgaaacgt tcgataagag ctgccatcac
     8701 ttcacgaacg gagattggac gagacaactc ctgtgtcatg cttgtcgtcc tggcgccctg
     8761 gataccgcac ggaacaatca gctcaaacgg cgccagatcc atattgacgt tcagcgcaaa
     8821 gccatgacgg gtgacccacc ggctgacatg aatgcctagt gaggcaatct tctcgtcgcc
     8881 cacccagacg cctgttctac cgagtgagcg accagcagtg attccgaagc ccgatagggt
     8941 catgatcagc acctcttcga gctgccgaag gtatcgatgc aggtcacgac catgttcaac
     9001 cagattgagg atcgggtatc ctactaactg gccagggccg tgataggtca tgtcgccacc
     9061 gcgctccact tcgaaaaact cgatccccag cgcggtcaag gagggttccg gtacgcgaag
     9121 gtgagccatc cgcccggctc gccctagggt gataaccggc gggtgttcga cgaggagcag
     9181 gacgtccccc aggcgatcat caacccgaag cgtcgccagc cgtcgttgca gcattaacga
     9241 ctcgtcgtat ggcacgagcc caaggtcgat caggctacag atcggcatca gtagtacagg
     9301 gtttagggta gagggtagag ggtttagggt gcagggaaag gaccggagca caactttcca
     9361 tacttctttt atcctatctt atcctctacc ctatacccta taccctatac cctctaccct
     9421 gccttataca tggacggccc ggccaagcgc ggcaagggcg gcctctccca tcgcctcgga
     9481 aagggttggg tgggcatgaa tcgacctggc aatctcttcc ggggtggcct ccaatgcgat
     9541 cgccatgccg g
//
LOCUS       DATMXI010000224         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_46421, whole genome shotgun sequence.
ACCESSION   DATMXI010000224 DATMXI010000000
VERSION     DATMXI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46421"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VN444_04225"
     CDS             <1..379
                     /locus_tag="VN444_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWR21051.1"
                     /translation="GSGGAALQPSAFSLQPVLDPGLVADICAGFQEAVVDVLVRVSLA
                     AAKVSTSRRLVLAGGVACNGRLRSKLTERAAEEGVQVYYPKPSLCTDNAAMIAAAGYP
                     RLLRGERALLSLNADANLALGLT"
     gene            390..893
                     /gene="def"
                     /locus_tag="VN444_04230"
     CDS             390..893
                     /gene="def"
                     /locus_tag="VN444_04230"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWR21052.1"
                     /translation="MAKLPILLYPSPVIRKKSLPVTSIDGELQRFIDDMVETMYAAPG
                     MGLAAPQVGALKRVIVLDPSDDRTSGRPMTLINPVLVAGEGQIVDEEGCLCIPDLTEP
                     ISRFRQVVVKAYDRNEKEIVLEGADLLARILQHEIDHLNGILFIDRLSTAKRLLLKRR
                     LKKAAKD"
     gene            complement(947..1987)
                     /locus_tag="VN444_04235"
     CDS             complement(947..1987)
                     /locus_tag="VN444_04235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWR21053.1"
                     /translation="MRQCEVRGAKTPHPPFTKGGYGGIWSAGCAAVALLFSILIASDS
                     EAVILDRVVAVVNDDVITLTEVQEEGLQAIRRIVRETLGIEREQQLRSTERQILDELI
                     LRRLQLQEAKKERIEATPAEVQSAIEELKKRNGLASDEDLWAALSRELLSEEQFRRGI
                     SEQVIMTKLVTRRVRSKVVVLDDEVQQYYEQQQGQFKDMSQFKIRHLLVAVPQQSTPS
                     EVKRAKNRIEEAQRLIKSGTNFSTVAQQYSEGPLAASGGEIWTMKRGELSPEVEQAAL
                     TLPIGQASDIITTPAGFHLIVVEERVPGLALLFDQVKEQIRTILFNQKTEATFKEWIE
                     SLKAKANVEMKL"
BASE COUNT          382 a          643 c          592 g          447 t
ORIGIN      
        1 gggttcaggg ggagcagccc ttcagccttc agccttcagc cttcagcctg ttcttgaccc
       61 tggtcttgtg gcggacatct gcgctggatt ccaagaggcg gtggtggatg tgctggttcg
      121 cgtgagcttg gcggcggcga aagtgtccac ctctcgccgt ctcgtcctgg ccggaggggt
      181 ggcctgcaat ggcaggctcc ggtcgaagct cacagagcgg gcggcagagg aaggcgtgca
      241 ggtctactac cccaagccgt cgctctgcac cgataacgcg gccatgatcg cggccgccgg
      301 ttaccctcgc cttctgcgcg gcgagcgagc cttgctttcg ttgaacgccg atgccaatct
      361 cgcgctcggc ctgacgtaag ggaacacgaa tggcgaagct accgatcctg ctctacccat
      421 cccccgtcat ccggaagaag tcgctgccag tcacatcgat cgatggtgaa cttcagcgct
      481 tcatcgatga catggtggaa accatgtatg cggccccagg catggggctt gccgctcccc
      541 aagtgggagc gctcaagcgg gtcatcgttc tggacccatc cgacgatcgc acgtcgggtc
      601 ggcctatgac ccttatcaat cccgtactgg tcgccggtga aggacagatc gtggatgagg
      661 agggatgtct ctgtatcccg gacctgacgg agccaatctc gcgattcagg caggtggtgg
      721 tgaaggccta tgatcggaat gagaaggaga tcgttctgga gggggccgac ctgctggcgc
      781 gaatcctaca gcatgagatc gatcacctca atggcatcct gttcatcgac cgcctgagca
      841 cggcaaagcg tctcctgctc aagcgacggc tcaaaaaggc cgctaaagac taaggtgtag
      901 gggcgctgct tgctgcgccc gctttttggc agggcaagcc ctgcccctac aatttcatct
      961 caacgttagc cttggccttg agactctcaa tccactcctt gaatgtggcc tcggtcttct
     1021 gattaaatag gatggtgcga atctgctcct tcacctgatc gaatagaaga gccagtccag
     1081 gaacccgctc ctcgacgaca atgaggtgga agccggccgg agtcgtgatg atgtcgctgg
     1141 cctgcccgat cggcagggta agcgcggcct gctccacctc cggagagagc tctccacgct
     1201 tcatcgtcca gatctcaccg ccggatgccg cgagcggccc ttcagagtac tgctgagcga
     1261 ccgtagagaa gttggtcccc gacttgataa gcctctgcgc ctcctcaatc cggttcttgg
     1321 cgcgctttac ctcactgggc gttgactgct gcggtacagc cacaaggaga tgacgaatct
     1381 tgaattggga catgtccttg aattgccctt gctgttgctc gtaatattgc tggacctcgt
     1441 cgtcaagaac gacgaccttg gatcgcaccc gcctcgttac cagcttcgtc atgatgacct
     1501 gctcggagat ccccctccga aactgctcct cgctgagcag ttctctggac agagcagccc
     1561 acagatcctc gtcgctggcc aacccgttgc gcttctttaa ttcctcaatg gcggactgaa
     1621 cctccgcagg ggtcgcctcg atcctctcct tctttgcctc ctggagttgc aacctccgga
     1681 gaatcagctc gtccaggatc tggcgttccg tgctacgaag ctgctgctct cgctctatcc
     1741 ccagcgtttc tcgcacgatc cttcggatgg cctgaagccc ttcctcttgc acctcggtca
     1801 gggtgatcac gtcatcattg acaacggcca cgacgcgatc gaggatgaca gcctcagagt
     1861 ccgaagctat gaggatgctg aacaggaggg ctacggcggc gcaccccgca ctccaaatcc
     1921 ccccgtaccc cccttttgta aagggggggt ggggggtttt tgcgcctcgc acctcgcact
     1981 gtctcatcga cactcctgaa gtcgcgacag cagggtctga gcggccgcga ttttgtcgcc
     2041 cccgactact gcgtattcca gggc
//
LOCUS       DATMXI010000225         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_138940, whole genome shotgun sequence.
ACCESSION   DATMXI010000225 DATMXI010000000
VERSION     DATMXI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138940"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VN444_04240"
     CDS             <1..421
                     /locus_tag="VN444_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21054.1"
                     /translation="RWAELLRDGPKNAKENEKADEWKEDIRQFLSNLMLRRGFSPAYQ
                     YTQEIEVPEDLLTDIVDLLVGGWASSKWSLKALTGNPEKDRRAWADTIEAGNHHVILA
                     DVTEWVSMRGRTRAYNAVPAIKCDIDLIGDVGMFLIG"
     gene            complement(488..763)
                     /locus_tag="VN444_04245"
     CDS             complement(488..763)
                     /locus_tag="VN444_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21055.1"
                     /translation="MTWNHRIIRNAPGGYGLYEVYYDDKGNITDWTTAPVDVSGDSPK
                     DIKALLTLMLKDCDRPILNATTLEKLKGGDMMAAKKKAAKPAKTKKK"
     gene            complement(777..1052)
                     /locus_tag="VN444_04250"
     CDS             complement(777..1052)
                     /locus_tag="VN444_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21056.1"
                     /translation="MSSYLTEAELDALQDSGLDPFTPFAIQNASMGQLSFARHYGGCR
                     YNGREYLYIPTTDELVRNDVVKWIVARRRETARAAKQEAEMKQMEMM"
     gene            complement(1045..1266)
                     /locus_tag="VN444_04255"
     CDS             complement(1045..1266)
                     /locus_tag="VN444_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21057.1"
                     /translation="MASTLKLYRVTCRGAGIGWVFFAVSTDATSAYRKVRDDLDEHNR
                     GYTSSRELERIELLAEQTCYPDCGTILYV"
     gene            complement(1266..1436)
                     /locus_tag="VN444_04260"
     CDS             complement(1266..1436)
                     /locus_tag="VN444_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21058.1"
                     /translation="MNIRDALSRALNHLAQARDALRNEQIESIDLLRTQIEMALDQGR
                     RLLRDLDAGRGI"
     gene            complement(1731..1943)
                     /locus_tag="VN444_04265"
     CDS             complement(1731..1943)
                     /locus_tag="VN444_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21059.1"
                     /translation="MTPEQQAAIIYAASARASIRALGMASENAERMLRGEMRAYSEDA
                     FLAIIREEGIDHDNVLLALGYVPPSL"
     gene            complement(1940..2176)
                     /locus_tag="VN444_04270"
     CDS             complement(1940..2176)
                     /locus_tag="VN444_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21060.1"
                     /translation="MKVVDLGWCMARLGDDGLVIVSPSCGGTATYEKAESVTISGTRN
                     LIALRDALNEAFPPITPEARPDRIREYRRVEMQP"
     gene            complement(2194..2808)
                     /locus_tag="VN444_04275"
     CDS             complement(2194..2808)
                     /locus_tag="VN444_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21061.1"
                     /translation="MNKLYLFGAFILLALASTVEAHKGYHYAGLAGTAPTDYTAAGMM
                     MPHSPIDISKHSHAIGWTILWDAYDTGQFVEIGIGYNRAESKKVVLWWASPQHPTIKK
                     VASVPFGTWVDVSLRKVPGEPYVIATWTWSGNEITKRIAVPGWTSNVGYHPTKAEIYS
                     KKSDHPNNVEIRFEDVTTAPGDTLRWHIEGPWEPYGDYSTFTVR"
     gene            complement(2805..>3010)
                     /locus_tag="VN444_04280"
     CDS             complement(2805..>3010)
                     /locus_tag="VN444_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21062.1"
                     /translation="LVIACLTGFLACYAWQGVKIEALAGNVEAWRQVAETEYRTHHNV
                     VKVNKAQAQVTSTQIEQRKETRP"
BASE COUNT          593 a          877 c          809 g          731 t
ORIGIN      
        1 acgatgggca gagctgttac gcgatgggcc gaagaatgcc aaggagaacg agaaggctga
       61 tgagtggaag gaagacatcc ggcaattcct ctcgaacctc atgctccgtc gaggattctc
      121 accggcctat cagtacaccc aagaaattga ggtcccggag gatctactga ctgacattgt
      181 agacctgcta gttggaggat gggcgtcatc gaagtggagc ttgaaggcgc tcacaggcaa
      241 tcccgagaag gatcgtcggg cgtgggcaga tacgatagag gctggcaacc atcacgtcat
      301 ccttgcagat gtgaccgagt gggtatccat gcggggccga acgagagcgt acaatgcagt
      361 acccgcgatc aagtgcgaca tcgacctgat cggtgacgtg ggcatgttcc tgattggata
      421 aaagacagcc cccgctcacg cttccagtcg tggacggggg ccgactcctt cggatctgta
      481 aacgcgctta tttcttcttc gtcttcgcag gcttcgcggc tttcttcttt gctgccatca
      541 tatcacctcc tttcagcttc tcaagtgttg ttgcgttgag aatcggccga tcacagtcct
      601 tgagcatgag cgtaagcagc gccttaatgt ccttcgggga gtcgccgcta acgtcaactg
      661 gagcagttgt ccagtcggtg atgttgccct tgtcatcata gtagacctcg tacaggccgt
      721 acccccctgg cgcattccga atgattcgat ggttccaggt catagcagct ccttttctac
      781 atcatctcca tctgcttcat ttccgcctct tgtttcgccg ctcttgctgt ctctcttcgc
      841 cgtgcgacaa tccatttcac cacatcgttt cgcaccagtt catcagtggt cgggatgtag
      901 agatactcgc gcccgttata ccgacatcca ccgtagtgcc gtgcaaacga gagttggccc
      961 atactcgcat tctggatcgc gaatggcgta aacgggtcta ggccgctgtc ctgaagggcg
     1021 tcgagttcag cctcggtgag gtagctagac atagagaatc gtcccgcaat cgggatagca
     1081 tgtctgttca gccagcaatt caatgcgttc tagttctctg gacgaagtgt aaccgcgatt
     1141 gtgttcatcg aggtcatccc gcacctttcg atacgcactg gtggcgtcag tggacacagc
     1201 gaagaaaacc catccaatgc cagcccctcg gcacgtgacg cggtagagtt tgagcgtgct
     1261 cgccatcaga ttcccctccc ggcatccagg tcgcggagca accgacgccc ctgatcgagc
     1321 gccatctcaa tctgcgtgcg tagcaaatct atgctctcga tctgctcgtt tcgcagcgca
     1381 tcgcgggcct gggcgagatg gttaagggca cggctgaggg catcgcggat gttcatattg
     1441 ttcacgctcc tttcgtaggc tgttatacgc atcttcgatc cggatgactt cacggatcag
     1501 ccatcggatt gcgtcgtcac catacaaacc gcgatcctta agccactgga acatctcatc
     1561 ctcgcggatg atcgttgtca tatcccttct ccttcttcgg cctgatcctg tctagtatcg
     1621 cctgcgtaca ctgccgacac agttcatgtt tgggacgctt ccgatacttc cccgacgtac
     1681 gcggtgactt tgcggggttg acgaccacga cgaccgttct cccgcactcg ctacaggctc
     1741 ggcgggacat agcctaaagc gagcagtaca ttgtcgtggt caatcccctc ttcgcggata
     1801 atggcaagga aggcatcttc gctatatgct cgcatttcgc cacgtagcat tctctctgca
     1861 ttttcacttg ccattccaag cgcccggatt gaggctctag cggaggcagc gtagataatg
     1921 gcggcttgct gttctggtgt catggttgca tctccacgcg ccgatattca cggatgcgat
     1981 ctgggcgtgc ttcaggagta attggtggaa acgcctcatt gagcgcgtca cggagagcaa
     2041 tcagattgcg cgttccgcta atagtgacgc tttctgcttt ttcatacgtc gccgtaccgc
     2101 cacatgaggg tgagacgatt acaagcccat catcgcccaa ccgcgccata caccatccca
     2161 ggtcgacgac tttcatagtc tctcccttcg ccgttatctg acagtaaacg tgctgtaatc
     2221 gccatagggc tcccacggcc cctcgatatg ccagcggagc gtatcgcctg gggccgtcgt
     2281 cacgtcctcg aagcgaatct cgacattatt agggtggtca cttttcttcg agtagatctc
     2341 cgccttcgtg gggtgatacc cgacgttact tgtccagccg ggtaccgcga tccgcttcgt
     2401 gatctcgttg ccgctccacg tccatgtcgc aatcacatag ggctcgccgg ggaccttacg
     2461 gagagagaca tcgacccatg ttccgaacgg cacactcgcc accttcttaa tcgtgggatg
     2521 ctgtggactg gcccaccaga gcaccacctt cttactctcg gcacgattat atccgattcc
     2581 gatctctaca aattggcctg tgtcgtaggc atcccacagg atcgtccagc caatagcgtg
     2641 gctgtgcttg ctgatgtcta tggggctgtg gggcatcatc atgcccgcgg cggtgtaatc
     2701 ggttggcgcg gtgccagcca gaccggcata gtggtagccc ttgtgcgcct cgacagtgga
     2761 ggcgagggcc agtaggatga atgcaccgaa taggtagagt ttgttcatgg tcgcgtctcc
     2821 tttcgctgtt caatctgtgt gctggtgact tgcgcttgag ccttgtttac cttcacgacg
     2881 ttatggtgcg tgcgatactc cgtttccgcc acctgccgcc atgcctcgac gttgcctgcc
     2941 agtgcctcga tcttgacccc ctgccaggca tagcaggcga ggaagccggt cagacacgcg
     3001 atcaccaagg
//
LOCUS       DATMXI010000226         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_307360, whole genome shotgun sequence.
ACCESSION   DATMXI010000226 DATMXI010000000
VERSION     DATMXI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307360"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN444_04285"
     CDS             <1..212
                     /locus_tag="VN444_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21063.1"
                     /translation="YARMLETIERVAATRMKRSPIDSNTACASGAPMGINVRDTPHPR
                     LPPQGGKESVEGDFLGNTQGNSRCA"
     gene            203..1138
                     /gene="fmt"
                     /locus_tag="VN444_04290"
     CDS             203..1138
                     /gene="fmt"
                     /locus_tag="VN444_04290"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWR21064.1"
                     /translation="MRVSFMGTPMFALPSLRALIAAGHDICLVVTQPDRPAGRGRVPT
                     PPPVKLAAQELGLPILQPEKVGEPAVISALQAAQPEAIVVVAYGQLLPKSILTLPPYG
                     CLNIHASLLPKYRGAAPIPWAIIQGENVTGITMMQIEARMDAGPILMQQPEPIGPRDT
                     AGTLGERLAVTGAELLCQALDQVVRRTAQPIPQDEQFATYAPKLLPADTHLDWVRDAR
                     DLDCLIRGLCPAPGAVTSFGGRRVKVLQVEAEAAAGSPPGAVCAIDRAKGVFVAAGQG
                     GLWLSQVQPENRRVMTAAEFASGYRVRPGDLFGSL"
     gene            1149..2507
                     /gene="rsmB"
                     /locus_tag="VN444_04295"
     CDS             1149..2507
                     /gene="rsmB"
                     /locus_tag="VN444_04295"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWR21065.1"
                     /translation="MHARRLAFEILTQVEEQRAYASLLLDARLQRSRLSPQERALATE
                     LTYGVLRWQGRLDYLLAAVTDRPWERVDPALRRLLRLGAYQILFLTRIPAYAAVNETV
                     ALTQEVIRSQLKPVAKAFVNAILRRLLERHATIRFPDPSGDPVGALAARWSHPPWLVA
                     RWLKRLGVEETEALLQANNDTPALSVMVNSLKRQSEEVQERLSKIAGSVTPGRFVPGA
                     FHLNDGAEALRDSAFADGWYFPMDEAAALPVLLLDPQPGDVVLDACAGGGGKTALLLG
                     QLAGRGRVIALDPSARAHRRLREARTRLSLDRVFPVQADARQAAQLFTRPVDRILVDA
                     PCSGLGTLRRHPERRWQQQEAGLAALACLQRELLHGVAPLLKPGGFLVYSTCSLEPEE
                     TDAVIETFLRNFPEFTAVNGPGSLPTTVAELIDLKGALRTWPHRHGCDGFYAIRLRRR
                     DR"
BASE COUNT          424 a          848 c          830 g          434 t
ORIGIN      
        1 attacgctcg gatgctggaa accatcgaga gggtagctgc cacaaggatg aaacgatcgc
       61 cgatcgattc caataccgca tgcgccagcg gcgcgcccat gggcataaac gtccgcgata
      121 ccccccatcc ccgccttccc ccgcaagggg ggaaggagag tgttgagggg gactttctgg
      181 gcaatacaca agggaacagc cgatgcgcgt gagctttatg ggtacaccaa tgttcgccct
      241 gccatcgctc agggcgctca ttgccgcggg ccacgacatc tgcctggttg tgacccaacc
      301 ggaccggccg gccggacgcg gtcgcgtacc taccccgcca ccggtgaagc tcgccgccca
      361 ggaactcgga ctcccgatcc tgcagccgga gaaggtgggc gagccggcgg tcatctcggc
      421 gctacaggcc gcccaaccgg aggccatcgt cgttgtcgcc tatggtcagc ttctgccgaa
      481 gtcgatcctc accctcccac cgtatggctg cctgaacatc catgcctcgc tcctgccgaa
      541 gtaccgcggt gcggcgccga tcccatgggc gattatccag ggagagaacg tcacagggat
      601 caccatgatg cagattgagg cgcggatgga tgccggtcct atcctgatgc agcagccgga
      661 gccgatcggg cctcgcgata ccgccgggac cctgggcgag cggctggccg tcactggggc
      721 tgagctgttg tgccaagccc tcgatcaagt ggtacgccgg acagcccagc cgattccgca
      781 ggatgagcag tttgcgacct atgcgccaaa gctgctcccc gctgatacgc acctggactg
      841 ggttcgcgac gcgcgagacc tcgactgctt gattcggggc ctgtgtccgg cgccgggagc
      901 cgtgaccagc ttcgggggac gccgcgtgaa ggtgctgcaa gtcgaggcag aggcggcagc
      961 cggctctccc ccaggcgccg tgtgtgcgat cgatcgcgcg aagggggtgt tcgtcgccgc
     1021 cgggcagggg gggctgtggc tgtctcaggt gcaaccggag aatcgccgtg tcatgaccgc
     1081 agccgagttt gccagtgggt accgtgtccg gcccggcgac ctgtttggct cactttagag
     1141 gcacgcagat gcacgcacgg cggctcgcat tcgagatatt gacccaggtg gaggagcagc
     1201 gggcctacgc aagtctgctc ctcgacgcga gacttcagcg ctcgcgcctg tcgccacagg
     1261 agcgggctct ggcaaccgag ctgacgtacg gcgtcctccg ctggcagggt cgtctcgact
     1321 acctgttggc cgccgtgacc gaccgcccat gggagcgggt cgatccggcg ctacgccgcc
     1381 tgctgcgcct gggcgcctat cagatcctct tcttgacgcg cataccggcc tatgccgccg
     1441 tgaacgagac cgttgccctg actcaagaag tcatacgcag ccagctcaag cctgtcgcca
     1501 aagccttcgt caatgcgatc ttgcgccgat tgctggagcg acatgccacg atccgctttc
     1561 ctgatccgtc cggcgatccg gtcggcgccc tcgcggcgcg ctggtcgcat ccgccctggc
     1621 tggttgcccg ctggctgaag cggctggggg tcgaagagac cgaagccctt cttcaagcca
     1681 acaacgacac cccggcgctg tccgtcatgg tcaacagcct gaagcgtcag tctgaggagg
     1741 tccaggagcg cctctccaag atcgcgggat cggtgacgcc gggccggttc gtcccaggcg
     1801 cctttcacct caacgatggc gccgaggcgc tgcgggattc ggcattcgcg gacggctggt
     1861 actttccgat ggatgaggcg gcagccttac cggtcctcct gctggatccg cagcccggcg
     1921 acgtcgtatt ggatgcctgc gccggcggcg gcgggaagac ggccctgctc ctgggacagc
     1981 tcgccggacg cgggcgagtg atcgcgctcg acccgagcgc tcgcgcccac cgacgcctcc
     2041 gagaggccag gacgcgcctg tctcttgatc gagtcttccc ggtccaggcc gatgccaggc
     2101 aggccgcgca gctcttcacg cgcccggtgg acaggatcct ggtggatgcg ccatgtagcg
     2161 gcctcggcac cttgagacgc catcccgagc gtaggtggca gcagcaagag gcaggcttgg
     2221 cagccctggc gtgtctgcaa cgtgagctgc tgcacggcgt cgcgccgctg ctcaagcctg
     2281 gaggattcct ggtctacagt acctgttccc tggagccgga ggagaccgac gccgtgattg
     2341 agacgttcct gcgtaacttc ccggagttta ccgccgtcaa cggcccaggc agcctcccta
     2401 caacggtcgc agagttgatc gacctcaagg gcgcgcttcg cacctggccg caccgccacg
     2461 ggtgcgacgg cttctacgcc atccgactgc gcaggcgaga ccgatgagac tcctccgact
     2521 gattggcaaa ggactg
//
LOCUS       DATMXI010000227         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_246198, whole genome shotgun sequence.
ACCESSION   DATMXI010000227 DATMXI010000000
VERSION     DATMXI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246198"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..494)
                     /locus_tag="VN444_04300"
     CDS             complement(261..494)
                     /locus_tag="VN444_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWR21066.1"
                     /translation="MRKTVEQAVGRQMVADVPVGAFLSGGLDSSAVVALARRHTTDRL
                     QCFTVDEPQADPAALSALFICKLSRSGFGARAG"
     gene            809..1975
                     /locus_tag="VN444_04305"
     CDS             809..1975
                     /locus_tag="VN444_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21067.1"
                     /translation="MPVVVVTGMRQTGKSTFLQQQPELQRRRFITLDDLSWLSAAREN
                     PEGLVDTDEPLIIDEAQRCPELFIAIKRAVDKKRTPGQFILSGSANFLLMKNIAESLA
                     GRAAYFNLHPFTRRELHGQTGEVPFLKRFFTEPTPVINGGEESAPITLDEVRQGGMPL
                     VSLGEVRDKDIWFKGYEQTYLERDIRDLSRIGNIIPFRNFLRLAAFRTGQLLRPSELG
                     RDARLTTATVSSYLSLLEVSCVMYRLAPYLRNRASRLVKSPKFYLGDSGLACYLAGTD
                     NLTDHPLKGAMMETYVAQNLVGLLDATWPHATISFWNIQGRHEVDFVIEVGHRCIAVE
                     TKASPLWDGKDLAGLKSFMAATPDCLAGILAHNGTTAVRIGERLWAIPLSLLLS"
BASE COUNT          478 a          740 c          616 g          476 t
ORIGIN      
        1 gtgtcagtcc ccatcgaccg atcagcggac tcaatcgact ccatcgtctc gcttttcccg
       61 ctcgtctcgc tcgtcctgca gatcatccag acgcaatcaa cgctgcacgc tgcacgcaat
      121 agacgcggca cgcgcacttc cacaccgggg gccatggaca ttttatccgt atctcaagca
      181 tagattggga ggtgtcattg cgaatggagt gacccaaatc cccccaaccc ccctttgata
      241 aaggggggcg agaggggatt tcaaccagcc ctcgcaccga atccggatcg agacagcttg
      301 cagatgaaca gggcgctcag ggcggcgggg tcggcctgcg gctcgtcgac ggtgaagcac
      361 tggagcctgt ccgtggtgtg ccgccgggcc agcgccacca ccgcgctcga atccaaccca
      421 cccgacagga aagcccctac cggcacgtcc gccaccatct ggcgccccac cgcctgctct
      481 accgtcttcc gcactgcctc gaccgcgctg ctctcagaca gcgcgccccg ctctcgaccg
      541 gagggccgat catagaaacg ccgaaacgcc agtgcccgcc tgtctgcgtg cggacacgca
      601 cagataggga cttaccacgc ctgccccgta cttgatacgg ggctttctac gctggattct
      661 tgctcccggc ttacaacatg ccgggacaag cttcactgga atgtcacttt agtagttgca
      721 tgcctgtcat tatgatgcta tattctatgt cactatgaca tatctagcgc gagatattac
      781 ccattcgata ctcgcggcgt tgcagagcat gccagtcgtc gtggtcaccg gcatgcgaca
      841 gactggaaaa agcaccttcc tccagcagca gccggaactc caacgaagaa gatttattac
      901 gctcgacgat ctttcctggc tgtccgccgc cagagaaaat cctgaaggat tggtggatac
      961 ggacgagcca ctcatcatcg acgaggcaca gcggtgtcct gagctgttca tcgccatcaa
     1021 gcgggcggtg gacaaaaaga ggacgcctgg ccaattcatc ctctccggct ccgccaattt
     1081 cctgctcatg aagaatatcg ccgagagtct cgcggggcgg gcggcgtact tcaacctgca
     1141 cccttttaca cgtcgcgaac tgcacggcca gacaggagaa gtccccttcc tcaaacggtt
     1201 ctttactgag ccgacgccgg tgatcaatgg gggggaggag tcggcgccga tcaccttgga
     1261 tgaggtgaga caaggcggca tgccgctggt cagtcttggc gaggtacggg acaaggacat
     1321 ctggttcaag ggatacgagc agacctacct ggagcgagac atccgcgacc tgagccgcat
     1381 aggcaacatc atccctttca ggaacttcct tcgcctggcg gcattccgga ccggccagct
     1441 cttacgtccc agcgagctgg gacgggatgc gcgcctcacc acggcaaccg tttccagcta
     1501 tctctctctc ctggaggtct cctgtgtgat gtatcgcctc gccccctatc tcaggaacag
     1561 agccagcaga ctcgtgaagt cgccaaagtt ctacctgggg gattcaggcc tggcgtgcta
     1621 cctggcgggt acggacaatc tcaccgacca cccgctgaaa ggggccatga tggaaaccta
     1681 cgtggcgcag aatcttgtgg gcctcctcga cgccacatgg cctcacgcca ccatctcctt
     1741 ctggaacatc cagggacgtc acgaggttga cttcgtcatc gaggtgggcc atcggtgcat
     1801 tgccgtggag accaaggcct cgcccctatg ggacgggaaa gacctcgcag ggctcaaatc
     1861 cttcatggcg gccacacccg actgcctcgc cggcatactc gcgcacaacg ggacgacagc
     1921 cgtcagaata ggcgagaggt tatgggcgat tccgctttcg ttactcctat cctgatgcgc
     1981 tggacatggg cgtgtgccat gcccaaggtg tgtacgcttc ctgacactgc ttgtagagca
     2041 actgtcacta ctcaggtggt gaggctgaag gcgatctcgg tttctacccc taacggcctt
     2101 cagtcttcag tctatctacc tgaatagcta tccaaaaacg tcctgcacca cacctcgatg
     2161 cacacgaccg cgaagatcgt gtaggccgcg tcaatatgac caaacagacc atcttggtct
     2221 atctcgtctt tctggtctat tccgtctctc tcgtcaccga ccacacggac cattttggtc
     2281 tatctcgtct ctctcgtctc ttccgtcttt
//
LOCUS       DATMXI010000228         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_265916, whole genome shotgun sequence.
ACCESSION   DATMXI010000228 DATMXI010000000
VERSION     DATMXI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265916"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VN444_04310"
     CDS             <1..902
                     /locus_tag="VN444_04310"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWR21068.1"
                     /translation="TRLLSSQLPELRAIGDELKDSCQRSPINLWGELCGQSAGAHEPL
                     APTLARHATSNDYQTSVYQDLAKYAKEALRGTGLDQPTAWGAPEPVDLIELHDPMDEI
                     VTTLLYRVSHASYRNILALVRSWTEKQKQEAVDVATSARGPYDELIKEFRSGYAFTFD
                     VLMDIGGWRDMHRHRRCQQIQQNFTTVHGYEVPPPLVQAGLDQEYRQAMDAVRSDIEQ
                     LRKTSAEGSLYATPFGFKVRCLFKMDYAEAEYIAKLRSGVKGHWSYRTVAWLMKQKLS
                     ARYPALGDRIQATSPDIEDTLTR"
     gene            1060..1959
                     /locus_tag="VN444_04315"
     CDS             1060..1959
                     /locus_tag="VN444_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21069.1"
                     /translation="MIHLLRGEFAEAWNTHSKCLQEPEDIAQIKAWIEGIVMQQSERA
                     NAHLVMGLFLAQSGQSEQSIACYKKAINLEPESAHPHYFLAQIHERANHLEMAIKEYR
                     EAVQLDPSYAPARTNLGVSYQEQGRLEMAIPQYREVIKLHPNDPVAHANLACALVEQG
                     KLEPALQSYKEALRLNPMDAAVHLALGGLYETRGRLDLAQKAYDAALIADPNFGPAHS
                     AIGWLALGKQQMQAAYESFNRALKSNAEDPRAYHGIAEYYSHKGKRESAMENFTNALK
                     YYKDPEMKNAILNQLFQEGQMAD"
BASE COUNT          497 a          592 c          575 g          387 t
ORIGIN      
        1 tcaccagact gttgtcatca cagctgccgg agcttcgtgc gatcggagac gaactcaaag
       61 attcctgcca acggtcaccc atcaatctct ggggtgagct gtgtggtcag tcggccggag
      121 cgcatgagcc cctcgccccg accctcgccc gccatgcgac gtcaaacgac taccagacct
      181 cggtctacca ggacctcgcc aaatacgcga aggaagcact caggggaacc gggttggatc
      241 aaccgacagc ctggggggcg ccggagccgg tggacctcat cgaactccac gatccaatgg
      301 acgaaatcgt gaccaccttg ctctaccgtg tctcacacgc atcctatcgg aacatcctcg
      361 cgctcgtccg ctcctggaca gaaaagcaaa aacaggaggc tgtcgacgtg gcgacgagcg
      421 cacgagggcc ttatgacgaa ctgatcaaag aatttcgaag cggttatgcg ttcacgttcg
      481 atgtgctgat ggacatcggg ggctggcgcg acatgcatcg gcatcggcgt tgtcagcaga
      541 ttcaacagaa ctttacgacg gtgcatggct atgaagtccc accaccgctc gtgcaggcag
      601 gattggatca ggagtaccgg caggcgatgg acgcggtccg ctcggacatt gaacaactga
      661 ggaaaacgag cgcggagggg tcgctctacg cgacgccgtt tggattcaaa gtgcgttgcc
      721 ttttcaagat ggactatgca gaagccgagt acatcgcgaa actgcgctct ggtgtgaaag
      781 gccactggtc gtaccggacg gtggcttggt tgatgaaaca gaaactttcg gcgcgctacc
      841 ccgcacttgg agatcgcatc caggcgacct cgccggacat tgaagacacc ctcacgagat
      901 aaccattcac gaccgatccc aatgaccgac tatcaagcac agtgtgaaac agcgctgttg
      961 gaaggccaat gggatcaggc gcaacaggcg gcgctggcct gggcccgcca tcccgtcagc
     1021 ggcacagaca gagatccgag gccccagttt gctctcaacg tgatccatct gctccgtgga
     1081 gagttcgccg aagcctggaa cacccattcg aagtgcctac aagaaccgga agatattgct
     1141 caaatcaaag cctggatcga aggtattgtg atgcagcagt cggagcgggc caacgcccat
     1201 ctcgtaatgg gattattcct ggcccaatca ggccagtcgg aacagtccat tgcctgttat
     1261 aagaaggcga tcaacctcga accagagtcc gcccatcctc attattttct tgcccagatt
     1321 catgagcggg ccaaccatct tgaaatggcg atcaaggaat acagagaggc cgtgcaactt
     1381 gatccttcct atgcgccggc gcggaccaat ctgggcgtgt cctatcaaga gcaaggacgg
     1441 ctggagatgg cgattcccca atatcgcgaa gtgatcaagt tgcacccaaa cgacccggtg
     1501 gcgcatgcca atcttgcctg cgcgcttgtc gagcagggca agttggagcc tgccttgcag
     1561 tcctataagg aggcattgcg cctgaacccc atggatgcag ctgtccatct tgcgctggga
     1621 ggtctctatg aaacgcgagg ccgtctggat ctggcacaga aggcgtatga cgcggctctc
     1681 atcgccgatc cgaatttcgg cccagcgcac tcggcaatcg gctggttggc gctgggcaaa
     1741 caacagatgc aggccgccta cgaatccttc aatcgcgccc tcaaatccaa cgccgaagat
     1801 cctcgagcct accatggcat tgctgagtat tattcccata aggggaaacg agagagcgca
     1861 atggaaaact ttaccaatgc gctcaagtac tacaaagatc ccgaaatgaa aaacgcgatt
     1921 ttgaatcagc tattccaaga aggccaaatg gcggattgag gacctgaggc tccttcttcc
     1981 ttgctcgcag aggccccacg atgaaacggt ggtccctcga tgcgcgcagt cgaagaagga
     2041 ggcctcaggc c
//
LOCUS       DATMXI010000229         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_306649, whole genome shotgun sequence.
ACCESSION   DATMXI010000229 DATMXI010000000
VERSION     DATMXI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306649"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1990
                     /locus_tag="VN444_04320"
     CDS             356..1990
                     /locus_tag="VN444_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWR21070.1"
                     /translation="MVELIVDGMPVQAPAGTSILEAVLRTGGEIPHFCYHPKLRVVGS
                     CRMCQVEVQGAPKLVISCATPVADGMEVFTASERVKKARNAVLEFLLLNHPLDCPVCD
                     KGGECPLQNYTLKFGPGESRFIEPKIHRIKHQPIGQFIIFDAERCILCTRCVRFCQDV
                     TGTSELGVFGRGDRSEIGLFPGQSLDNSYSGNVIDLCPVGALTSRDYRFQARPWDLVK
                     QVPSICGLCSAGCNITVDVRHKEQGRQILRIRPRVNNEVNGHWICDEGRFEFHFAEDP
                     GRIGQPLIRRDGELLPVSWGEAIGRIAGDIARILREKGAEAIGVIISARLTNEEAFLI
                     QQLFGERLGILNMDYRVRRSQEPGGDAPEDHLLRRTDKHPNSVGMRTLGLLPGAHGMG
                     TKKMLGAATEGRLAALLVFGEDLVTALSGELAVAESLKNLELLVAHDLFLTATAKLAH
                     VVLPGLSFYEKEGTFTNFAGRVQRLQPALEPFGRAISLAEILQRLACQLELPLVEGNA
                     EAVWNEIAQSVSPYAGVTYESIGELGTPLTDTLAEG"
     gene            2188..2715
                     /locus_tag="VN444_04325"
     CDS             2188..2715
                     /locus_tag="VN444_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWR21071.1"
                     /translation="MGLFDNKQIEPGVVTTTVEWLFNWARKSSPWPMTFGLACCAIEM
                     MAAGASRFDLDRFGAGVFRPSPRQSDVMIVAGTVTEKMAPRIKTLYEQMPDPKWVIAM
                     GACAISGGPFYYDTYHVVKGVDLLVPVDVYVPGCPPTPEALIFGILTLQEQITRGKRA
                     QPGGRPTLPEPLVAA"
     gene            2726..3262
                     /locus_tag="VN444_04330"
     CDS             2726..3262
                     /locus_tag="VN444_04330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWR21072.1"
                     /translation="MGRYFSDLFGGSASILRSMWVTLRYLFTHAITVQYPDEHRPQPL
                     RALNRHVLRTDETTGQLKCTACEACARICPTRCIELTGTGKGKNRHPSAFTIDHNLCM
                     YCNLCVEVCPFDAITMWTRIGELSSDARSGLVYDLKALTADRYYPSPMTPEKPSVPEK
                     PPVPEPEKVEAGATVPSS"
BASE COUNT          672 a          927 c          986 g          747 t
ORIGIN      
        1 gcctcccgac cttgacgcca aagtgctctg aaacgccaag ccttcctttc tcctacctca
       61 tctctcaatt acgccttctt agtatcttta tgatagtcct cgtgtgacgc gaagtcaagg
      121 tgaaagcctg gatggatggc gtgcgaaacg tgtagaatcg gctgctttgc agttgacagt
      181 ctccggaagc tctactatat ttagctagaa aatgtctgtt tcgtctatct cgtctgttcg
      241 gtctgttccg tcgtttggtc tagaccaaat ggaccagata gaccaaagag acccagaatt
      301 ctccagaatt gcattatcgt gggttgtggt gaggcctagc ctcatagggt gtgacttggt
      361 tgagctgatc gttgatggta tgccggtgca ggctcccgcg gggacgtcaa ttctcgaggc
      421 ggttctgcgg accggtggcg agatcccaca tttttgctat catccaaagc tccgcgtggt
      481 gggcagttgt cggatgtgcc aggtggaggt gcagggagcc ccaaagctgg tgatctcatg
      541 cgctacccca gttgcggatg ggatggaggt gttcacagcc tctgaacgcg tgaaaaaggc
      601 caggaatgcg gtgctcgagt ttctgttgct gaatcaccca ctcgattgtc ccgtctgcga
      661 taagggtgga gagtgtccgc ttcagaatta cacactcaag ttcggcccag gcgaaagccg
      721 ttttatcgaa ccgaagatcc atcgcatcaa gcatcagccg atcggccagt ttatcatctt
      781 cgacgcggag cggtgcattc tgtgtacgcg ctgtgtccga ttttgtcagg atgtgactgg
      841 cacttcagag ctgggcgtgt tcggccgagg cgaccgatcc gagatcggcc tcttccctgg
      901 tcagagcctg gataacagct actcgggaaa cgtgatcgac ctctgccctg taggggcact
      961 gacaagccgg gactatcgct ttcaggcgcg gccatgggat ctggtcaaac aggttccgtc
     1021 gatatgtggg ctgtgcagcg ccgggtgcaa cattacagtg gatgttcggc ataaggagca
     1081 gggccggcag attctccgca tccgccccag agtgaacaat gaggtcaacg ggcactggat
     1141 ctgtgacgag ggccgcttcg agtttcattt tgctgaagac ccaggacgga tcggtcagcc
     1201 attgatacgg cgagatggtg agttgctgcc ggtgagttgg ggcgaggcga tcggccgtat
     1261 tgccggcgac atcgctcgaa tcctccggga gaagggagcc gaggccatcg gcgtgatcat
     1321 atcggcacgg ctgaccaacg aggaggcatt cctcattcaa caactgtttg gggagaggct
     1381 cggtattcta aacatggatt accgggtgag acgatctcag gagcctgggg gtgatgcgcc
     1441 ggaagatcat ctgcttcggc gaactgataa gcaccctaat tcggtcggca tgcgtacttt
     1501 gggacttctt cccggagccc atgggatggg gaccaaaaag atgctgggcg ccgcaaccga
     1561 aggtcggttg gctgccttgc tcgtctttgg tgaggatctg gtgactgcac tgtcgggaga
     1621 actcgccgtt gcggagtcgc tgaagaatct agaactgctg gtcgctcacg atttattctt
     1681 gacagcgacg gcaaagctgg cgcatgttgt ccttccggga ttgagctttt acgaaaaaga
     1741 ggggacgttc acaaattttg cagggcgggt tcagcgcctc cagcctgcgc tggaaccatt
     1801 tggtcgcgcg atttcgcttg ccgagatcct tcagcgtctc gcatgccaac tggaacttcc
     1861 cctcgtggaa gggaacgcag aagcggtgtg gaatgagatc gcccagtcgg tttcgccata
     1921 tgcaggcgtg acgtacgaga gtattggtga gttgggaacg ccgttgacgg atacgctggc
     1981 ggaaggttag cggctgtagt agtgtagcgt atacaacgct tctttacatt aatttcgaag
     2041 ccgtcattcc cgcgaaagcg ggaatccagg acagaaaaac tggattccgg gtcaagcccg
     2101 gaatgacgat cagagtaaag agacttttgg tacaccacac tcgaatcgat agacgcgata
     2161 gacattttcc gagcaaggag atttctgatg ggattgttcg acaacaaaca aattgagccg
     2221 ggtgttgtca ctacgacggt cgagtggctt ttcaactggg cccgtaagtc ttccccctgg
     2281 ccgatgacct ttggtctggc ctgctgtgcg atcgagatga tggccgccgg cgcttcccgc
     2341 ttcgatcttg accgttttgg ggcgggagtc tttcgcccat cgccgcgcca gtccgacgtc
     2401 atgatcgtcg ctgggaccgt taccgagaag atggctcctc gcatcaagac gctttacgag
     2461 cagatgcctg accccaagtg ggtcatcgcc atgggcgcct gcgccatttc cggcggccca
     2521 ttctactacg atacctacca cgttgtgaag ggggtggacc tgctcgttcc ggtcgatgtc
     2581 tacgttcccg gctgcccacc cacccctgag gccctcatct tcggtatcct caccctccag
     2641 gagcagatca cccgtggcaa gcgcgcacaa cccggtggcc ggccgaccct gcccgagcct
     2701 ctggtggcgg cctaaggggg acaccatggg acgctacttc tcagacctgt tcggcggcag
     2761 cgcgagcatc cttcgctcca tgtgggtaac gctccgctac ctcttcaccc acgctattac
     2821 cgttcagtac cccgatgagc atcggccgca accgttgcgc gcgttgaacc gccacgtact
     2881 gaggaccgac gagacgaccg gacagctcaa gtgcaccgcc tgcgaggcct gcgcccggat
     2941 ctgtcccacc cgctgcatcg agctgaccgg taccggcaag ggcaagaatc gccatccgtc
     3001 cgcctttact atcgaccaca acctctgcat gtactgtaac ctttgtgtcg aggtctgccc
     3061 ctttgacgcc attacgatgt ggacccggat cggtgagctc agctccgacg cgcgcagcgg
     3121 cttggtctac gatctgaagg ccctgaccgc cgaccgctac tacccctctc cgatgacccc
     3181 agaaaagccg tcagttcccg aaaagccgcc ggttccagag cccgagaagg tggaggctgg
     3241 agctacggta ccctcttcct gagatgagta ctccagtgga agaggtaatc attcttggtt
     3301 caggcccggc gggcctgacg gcggccttat ac
//
LOCUS       DATMXI010000230         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_326368, whole genome shotgun sequence.
ACCESSION   DATMXI010000230 DATMXI010000000
VERSION     DATMXI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326368"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VN444_04335"
     CDS             <1..832
                     /locus_tag="VN444_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWR21073.1"
                     /translation="DKGGVEGFRTQHSPEVSGLQVEGHLRSDVLRWGNLVLTGAEGDL
                     GYCCGQLTIRQLKGGFFGGNLSGDAALSFNGRAPRVRVTTHLEGVQTEPFMKAIQEPR
                     WTLRGMMTLDSKMEFSGPLGPGALAKVSGQTDMAVMNGRVTGYAPLDKLSKTADPILK
                     SLGIPSLNLNEFDRLSAHWTLDSGILRTRDLTLLRDGAKFYAVGSANLLNQTLDFDVT
                     AKLAKTTLEAKVQGPSSDPVVTPQMGRIEGRIKTEVGKLLGKDRDKELGKMLKQLFNR
                     "
     gene            841..1887
                     /locus_tag="VN444_04340"
     CDS             841..1887
                     /locus_tag="VN444_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR21074.1"
                     /translation="MRLGIVTPFYFPSVRGNSITVQRIESGLREQGVNVRVWSLEDGP
                     PPGEILQALVAFTPDLVHGFHISASGRIVTDAAFRLGIPSILSVTGTDVNTDLFDPSR
                     RTEVMDVLQRATRIVVFHNVMRIKLVGELPQVEDRIRVIGQTVRYQETPFDLRRRLGL
                     TPDDLIFLIPAGIRRVKDVTFCLKPLDALRIQYPQVKAVFVGPVIEKAEGIRLREALR
                     DYPWAFYLGPVPHEQLCAMLSSVDVVINSSLSEGGMANSILEAMSRGRAVLASDIEGN
                     RSVVRDGIDGLLFASESEFACKAERLIREPGLRDELGRNGKEKIEREFSPEREIQDYL
                     RLYQEVLGACSHSE"
     gene            1898..2797
                     /locus_tag="VN444_04345"
     CDS             1898..2797
                     /locus_tag="VN444_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HWR21075.1"
                     /translation="MDLHTLELFCRIVESGSFSKAANAFYLTQPTVSGHIKKLEEEVG
                     VRLLDRLGHRTTPTKAGDLLYHYAKRILALRQETQQALDELRGGLKGELILGASSVPG
                     GYLLPPLIGRFRAQYPDISVVLKVSDSKEIIEAVITGAYEVGAVGAQFDDGRIEYQKF
                     AEDEMVLVVPPTHPWASRRSVRVKELPSQPFLIRERGSGTRKILEQALEQHHLSMGAF
                     KVIGEMGSNEAIRQAVKTGGGIAIISKLAVASDLKCGELTAIPVAGLKLTRPFYLVTH
                     RHRSRSPICNAFLAFLDTPTPSG"
BASE COUNT          633 a          845 c          875 g          569 t
ORIGIN      
        1 tgataaaggg ggggtggagg gatttcgcac tcagcactcc ccagaagtca gcggcctcca
       61 ggttgaggga cacctgcgct ccgatgtcct gcgttggggg aatctggtcc tgaccggcgc
      121 agaaggcgac cttggttact gttgtggaca gctcaccatc cgtcaactca agggcggttt
      181 cttcggcgga aatttatcag gcgatgcagc gctctccttc aatgggcggg cgccccgtgt
      241 aagagtgacc acgcatctgg agggggtcca gactgaaccg ttcatgaagg cgatccagga
      301 gccgcggtgg accttacgcg gaatgatgac gctggactcg aagatggagt tttcgggacc
      361 gctcggccct ggggcgctcg ccaaggtctc aggacaaacc gacatggccg tgatgaacgg
      421 gcgcgtgacc ggctacgctc ctttagacaa gctctccaaa actgcggatc ctatcctgaa
      481 gagcttgggc attccttccc tcaatctgaa cgagttcgac cgcctcagcg cccactggac
      541 cctcgacagt gggatcctgc gaacccgtga tctgacacta cttcgggacg gggccaagtt
      601 ctatgctgtc ggaagcgcca atctgctcaa tcagaccctg gatttcgacg tcacggccaa
      661 gctggcgaaa acgacgcttg aggccaaggt acaaggaccc tcatccgatc cggtcgtcac
      721 gcctcaaatg ggtcgcatcg agggacgcat caaaacagaa gtcggcaaac tcctggggaa
      781 agatcgggac aaggagctgg ggaagatgct aaagcaactc ttcaaccgat gaggaggcag
      841 atgcgactgg gtattgtcac cccattttac ttcccctccg ttcgtggcaa ttcgatcacg
      901 gtccagcgta tcgaatcggg gttgcgggag caaggcgtaa acgtccgcgt ctggtccctg
      961 gaggatggcc ccccgcctgg ggagatcctg caggcattag tagcattcac tcctgatctg
     1021 gtccatggat tccatatcag tgcgtcgggg cggatcgtca cggatgctgc gtttcggttg
     1081 ggtatcccct cgatcctcag cgtaaccgga acagatgtca acaccgacct cttcgatcct
     1141 tccaggagga cggaggtcat ggacgtcctt caacgggcaa cgcggatcgt ggtctttcac
     1201 aacgtgatgc ggatcaagct tgtcggcgaa ctgccgcagg tagaagaccg tatccgggta
     1261 atcggccaga cagtacgcta tcaggagacc ccattcgatc tccggcgccg gttaggtctt
     1321 acgccggatg atctgatctt cctcattccc gcaggcatcc ggcgcgtaaa agatgtcacc
     1381 ttctgcctga agccactgga tgcgctgaga atacagtacc cccaggtcaa agccgtcttc
     1441 gtaggaccgg tcatcgaaaa ggctgaaggg atcagattac gggaagcctt acgggactat
     1501 ccctgggcct tctacctggg accggtccct cacgaacagc tctgcgccat gctttcgtcc
     1561 gttgatgtgg tcattaactc ctcgctctcg gaaggcggca tggcgaatag catcctggag
     1621 gcgatgagtc gggggcgagc ggtcctcgcc tcggatatcg aggggaaccg atcggtcgta
     1681 cgagacggga tcgatgggct cctcttcgcg tcagagtcgg agttcgcctg caaggcggaa
     1741 cggctcatca gagaacccgg cctccgcgat gaactcggga ggaacggaaa ggagaagatc
     1801 gaacgggagt tctccccgga aagagagatt caggactatc tccgcctgta tcaggaagtg
     1861 ctcggcgcct gctcccacag cgagtagaag acgcatcatg gatctccaca ccctcgaact
     1921 gttttgcagg atcgtcgaat ctggaagctt ctcgaaggca gcgaacgcgt tctacttgac
     1981 tcaacctacg gtgagtggtc acatcaagaa actggaggaa gaggtggggg tcaggctcct
     2041 cgatcgcctc ggacaccgga cgaccccaac caaggccgga gacctgctgt atcactatgc
     2101 taagcggatc ctagcgctcc gtcaagaaac ccaacaggcc ctcgacgagc tgagaggagg
     2161 gctgaaagga gaactgatac tcggcgccag cagcgtccca ggcggctatt tgctgccgcc
     2221 cctgatcggg cggttcaggg cccagtatcc ggacatctcc gttgtcctca aggtctcaga
     2281 ctcaaaagag atcatcgaag cggtgatcac cggcgcgtac gaggtcggcg ccgtaggcgc
     2341 acaattcgac gatggcagga tcgagtacca gaaatttgcc gaagacgaga tggtcctggt
     2401 tgtgccaccc acgcaccctt gggcgtcccg aaggagcgtc agggtgaagg agctgccgag
     2461 ccagccgttt ctcattcggg agcgaggctc gggaacgcgc aagattctgg agcaggcatt
     2521 agagcagcac catctgtcga tgggagcctt caaggtgatc ggagagatgg gcagtaacga
     2581 ggcgatccgg caggcggtca agacaggcgg cggcatcgcc atcatctcga agctcgccgt
     2641 tgcgagcgac ctcaagtgcg gcgaactcac cgccattccc gtcgcaggcc tgaaactgac
     2701 cagaccgttc tacctcgtca cccatcgcca tcgttcgcgc tcacctatct gcaacgcctt
     2761 tctcgccttt ctcgacaccc ccaccccatc agggtaagca ccgcttattt gagatgctac
     2821 gctatcaaac caagacgacg tagcgcttct tcgttccagt tggtggcggc aaggatttgt
     2881 cggagagtgc cgctgggcag cgtgtctcca ccgtggtgat ag
//
LOCUS       DATMXI010000231         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_262233, whole genome shotgun sequence.
ACCESSION   DATMXI010000231 DATMXI010000000
VERSION     DATMXI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262233"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="aroE"
                     /locus_tag="VN444_04350"
     CDS             <1..687
                     /gene="aroE"
                     /locus_tag="VN444_04350"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWR21076.1"
                     /translation="GMDNFGGANVTIPHKEQAFALVDTLTKETAAVGAVNTVIAIGGR
                     LLGANTDGQGFLRSLHEEAAFLPHGKPAVILGAGGAARAVSVSLAEAGAEEIVIVNRT
                     IERAQSLAGFVNRETGVSAIGLGLDDPRMPARVRDCALVVNATSVGLSPSDPSPIDPS
                     LLQPGMLVYDLIYRPRETALLREATKRGCQVMGGLGMLLYQGALAFELWTGQKPSGEA
                     MRQALVAAIA"
     gene            776..2473
                     /gene="pilB"
                     /locus_tag="VN444_04355"
     CDS             776..2473
                     /gene="pilB"
                     /locus_tag="VN444_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HWR21077.1"
                     /translation="MAQVRVGEMLVKANLITPEQLDQALAKQKTTSERIGVILAKLGY
                     ISEEEISSCLGRQYGVPSVDLGTIEIDPAVISLISGEVARKHQLIPLSWSGSSLTVAM
                     ADPSDFGAIDELRFITGHNIQPMVASNVTIQNTLNRYYDSAEVAQGLVKDFQQTEMVG
                     DEPDHGEINLAELYRSTEDAPVVKLVNLILTDAINKGASDIHLEPYEKTFRIRFRLDG
                     ILYEVMSPPLTLHAAVISRVKIMARLDIAERRMPQDGRIKVKMLKRELDLRISTVPTL
                     FGEKVVMRLLDRASIELDMSKLGLDPEELVRLEKAVLAPHGMILVTGPTGSGKTTTLY
                     SALTRLNSIGTNIMTAEDPIEYNLTGINQAQVKPDIGLTFASLLRSFLRQDPDIIMVG
                     EIRDVETAEIAIKAALTGHLVLSTVHTNDAISTVGRLISMGVPAYLVSASLNLLLAQR
                     LVRRLCAECRAEVSVPVSSLEDIGFSPQEAKTLTCYRGKGCIACRDTGYRGRIGLYEV
                     VLLNEQMREAILTGASVSELRLLQAKYGMRSLVESGLQKIRQGITTVEEVVRVTSFG"
BASE COUNT          516 a          692 c          792 g          501 t
ORIGIN      
        1 gggatggaca acttcggagg cgcgaacgtc acgattcccc acaaggagca ggccttcgcg
       61 ctcgtcgata ccctgacaaa agagaccgcc gcggtgggcg cggtcaatac agttattgcc
      121 ataggtggac gtctactcgg agccaatact gacggacagg ggtttctccg atcgcttcat
      181 gaggaggcgg cctttctgcc gcatgggaag cctgctgtca tccttggggc aggcggggcg
      241 gcacgggctg tatccgtcag tctggccgag gctggcgctg aagagatcgt tattgtcaac
      301 cggaccatcg agcgcgcgca gtcgctggct gggttcgtga accgggaaac cggggtctcg
      361 gctatcggac tggggcttga cgatcctcgg atgccggcca gggtgagaga ctgtgccctg
      421 gtggtcaatg ccacatcggt tgggctcagc ccttcagatc cttcaccaat cgacccgagc
      481 cttcttcagc ccgggatgct ggtatacgac ctgatctacc gtccgcggga gaccgcgtta
      541 ctcagggagg caacgaagcg ggggtgtcag gtcatgggtg gactggggat gttgctgtac
      601 cagggcgcct tggccttcga gctgtggacc gggcaaaagc cttcagggga ggccatgcgg
      661 caagccctcg ttgctgcgat tgcttgactt atctgaagta tggtgaaata gtgttgtagc
      721 gcgaatgact gtgttagtcg tgatgcgaca gcgctaggca tgaaaggata gatgcatggc
      781 acaagtacga gtgggcgaga tgctggtgaa ggccaacctg atcacgcccg agcagttaga
      841 tcaggccctc gcaaagcaga agactaccag cgagcggatt ggagtgattc tcgccaagct
      901 cgggtatatc agcgaggagg agatctcatc gtgtctaggc cgccagtacg gggtgccgtc
      961 cgttgatctg ggcacgatcg agatcgatcc tgctgtcatc agtctcattt ctggcgaggt
     1021 ggctcgtaaa catcagctca tccctctgag ttggtccggg agcagcctca cggttgctat
     1081 ggcggacccg tccgacttcg gtgccatcga tgaactcaga ttcatcaccg gccacaacat
     1141 acaaccgatg gtcgcctcga acgtcacgat ccagaatacc ctcaataggt actatgactc
     1201 tgccgaagtc gcccagggct tggtcaagga ctttcagcaa acggagatgg tgggcgacga
     1261 gccggatcat ggggaaatta atctggccga gctgtatcga tccaccgaag acgccccggt
     1321 ggtgaagctg gtcaacctca tcctgaccga cgcgatcaac aagggcgcct ctgatattca
     1381 cctggagccc tacgagaaaa cattccggat tcgcttcaga ctcgatggca tcctctatga
     1441 ggtgatgtcc ccgcccctca cgctccacgc cgcggtcatc tctcgcgtca agatcatggc
     1501 gcgattggat attgctgaac ggcgcatgcc tcaggacggg cggatcaagg tcaagatgtt
     1561 aaagcgggag ctggaccttc ggatctctac agttccgacg ctgtttggcg agaaggtggt
     1621 catgcgactg ttggaccgcg cgagcatcga attggacatg agcaagctcg ggctcgatcc
     1681 tgaggagctg gtcaggctcg aaaaggctgt cctcgccccg cacggcatga tcctggtgac
     1741 cggcccgacc gggagcggga agacgaccac gctgtattcg gcgcttaccc ggctcaacag
     1801 tatcggtacc aacatcatga ccgcggaaga cccgatcgag tacaacctga ccgggatcaa
     1861 ccaggctcag gtcaagccgg atatcggcct gacctttgcg tccctactca ggtcgttcct
     1921 ccgacaagat ccggatatca tcatggtggg agagatccgc gatgtcgaga cggccgagat
     1981 cgcgatcaag gctgccctta ccggccacct ggtgttgagc acagtccata ccaacgacgc
     2041 aattagtacg gtggggcgac tgatcagtat gggcgtgccg gcgtatctgg tgagcgcctc
     2101 gctcaacctg ctgctcgccc agcgtctggt ccggcggctc tgcgcggagt gccgggcgga
     2161 ggtctcggtt cccgtctcct ccctggagga tatcggtttc agtccgcagg aggcgaagac
     2221 cctgacctgc tatcggggaa aggggtgcat cgcctgtcgt gacaccggct atcggggcag
     2281 gatcggcctc tacgaggtag tgctgctcaa cgaacagatg cgggaggcga ttctgactgg
     2341 cgcctccgtc agtgagcttc gtctgcttca agccaagtac gggatgagga gcttggtgga
     2401 gagcggcctg caaaagattc ggcaaggcat caccactgtc gaggaggtcg ttcgagttac
     2461 cagcttcggc taatacaggt agataggctg aaggctgaag g
//
LOCUS       DATMXI010000232         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_115376, whole genome shotgun sequence.
ACCESSION   DATMXI010000232 DATMXI010000000
VERSION     DATMXI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115376"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..329
                     /gene="rpsT"
                     /locus_tag="VN444_04360"
     CDS             57..329
                     /gene="rpsT"
                     /locus_tag="VN444_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWR21078.1"
                     /translation="MPIIKSAQKHMRQSQKRRLRNRAAKSSLRTVIKKVRVGIEERDR
                     DAAQKAYLLAVPLIDRAASKGFIHKNAAARYKSRLALQLQALPSPS"
     gene            complement(333..1337)
                     /gene="holA"
                     /locus_tag="VN444_04365"
     CDS             complement(333..1337)
                     /gene="holA"
                     /locus_tag="VN444_04365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWR21079.1"
                     /translation="MGRLVKTGHSVAEQVRRGEIALVYCLYGEDEYRREQALNQLLDA
                     LLTEGERDLNLDQIRPGEAETRSILGSARTLPFLGSRRVVLVRGVEDLSRAQQEELLA
                     YLNDPSPTSCLVLAGKSLDLRTRLAAAIQKKGMVLHFDHMKADSLREALVAAAKERGV
                     RLQPDATSLLIELVGDDLRQLIYAVEKLALFVGEGVEIRPHDIEAMVGETRVRSIFQL
                     TDTVSGRDLDGALRSLTSLLETGEEPLKILGMLARQIRLLFQAKALREQGTPVSRMTI
                     GRLSPRVVATLAEQGASRSWRQLTDALQSISRADMAIKTGRAEAPMVLNELVWGLCRT
                     "
     gene            complement(1337..1843)
                     /locus_tag="VN444_04370"
     CDS             complement(1337..1843)
                     /locus_tag="VN444_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HWR21080.1"
                     /translation="MRAIICMAVLLALAGCGYRSLGSGEVSALQPSVKTISIGILKNR
                     TLRPTIHPALKDALIRRFATDGRIRVVEAGGDVLLDGTIEGFGEDALAFSSSDQAKRV
                     RLSISFSFTVKNGLEDKVLLRDGVAGVAYYFTGSGVTSTRSAEDEATLRAVADLADQV
                     VSRVLDGV"
     gene            complement(1843..>2217)
                     /locus_tag="VN444_04375"
     CDS             complement(1843..>2217)
                     /locus_tag="VN444_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWR21081.1"
                     /translation="PTDEADERRFAYSYAVETLLLLLSPFAPHLCEELWERLGRSGSI
                     FHAAWPTYDPAVITAEEVVVVVQVDGKVRSRLFMPAGADDGAMREAALADERVKTWLA
                     DRPTRKVIVVPKKLVNIVTGGV"
BASE COUNT          470 a          736 c          600 g          411 t
ORIGIN      
        1 atttatcctc ccgtgtaccg gggggacacg acacgtcata aaggagaaga ggaacaatgc
       61 caataatcaa gtcagcgcag aagcacatga ggcaaagcca gaagcgacgg ttgcgcaatc
      121 gcgcagcgaa gagcagctta aggacagtca tcaaaaaggt gcgagtgggg atagaggagc
      181 gcgacaggga tgctgcgcag aaggcatact tactggccgt gccgttaatt gacagggccg
      241 ccagcaaagg cttcatccac aagaacgccg cggcccgcta taagtcaaga ctcgctctcc
      301 agctccaggc ccttccgtct ccgtcgtagg cctcacgtcc tgcagaggcc ccagaccagt
      361 tcgttcaaga ccataggggc ttctgctctc cccgtcttga tggccatgtc ggcccgtgag
      421 atcgactgga gggcgtctgt gagctgcctc caggaacgcg aggcgccttg ttccgccaag
      481 gtcgcgacaa cgcgaggcga taggcggccg atggtcatcc tgctcaccgg ggtcccctgc
      541 tcccgtaatg cttttgcctg aaacagcagg cggatctggc gggccagcat cccgaggatt
      601 tttaagggct cttcgccggt ctccaagaga ctcgtgagac tgcgaagcgc cccatccagg
      661 tcccttcctg atacggtatc ggtcagttgg aagatcgatc gtacccgggt ctcaccaacc
      721 atggcttcga tatcgtgcgg ccgtatttcc acgccctccc ccacaaagag cgcgagcttt
      781 tccacggcat atatcaactg gcgaagatcg tcaccgacca gctcaatgag cagactggtc
      841 gcgtccggct gcaaccgtac gccccgctcc ttggccgctg ccaccagcgc ctcccgtaac
      901 gaatcggcct tcatgtgatc aaagtgaagg accattcctt tcttttgaat cgccgccgcc
      961 aatcgggtcc tgaggtcaag actcttcccc gccagcacca ggcaacttgt gggagatgga
     1021 tcgttcagat aggcgagcaa ctcctcctgc tgcgcccgcg agagatcctc gacacctcgg
     1081 accaggacaa cccgacgcga ccccagaaac ggtagcgtcc gagcgctccc caggatcgac
     1141 cgtgtctctg cctcccccgg ccgaatctgg tcgagattca ggtctctctc gccctccgtc
     1201 agcaatgcat caaggagctg attaagcgcc tgttcccggc ggtactcatc ctccccgtag
     1261 agacagtaga cgagcgcaat ctcccctctg cggacctgtt cggcaaccga gtgacctgtc
     1321 ttgaccaagc gtcccatcac accccgtcca gcaccctact caccacctgg tcggccaggt
     1381 ccgccaccgc ccgaagcgtg gcctcatcct cagccgacct ggtagaagtg actcctgatc
     1441 ccgtaaagta gtaggccaca cctgcaacgc cgtcccggag gagcacctta tcctccagcc
     1501 catttttcac cgtaaaggaa aacgagatgc tcaaccgcac gcgcttggcc tggtctgagg
     1561 aactaaaggc aagggcatct tcaccgaacc cttcaatggt cccatccaaa aggacgtcgc
     1621 cgcccgcctc cacaactcgg atccggccgt cggtcgcaaa cctgcgtatc agcgcatctt
     1681 tcagggcggg gtgaatggtg ggacgaaggg ttcggttttt gaggatccca atactgatcg
     1741 tcttgacgga gggctgaaga gcgctcacct ccccggatcc cagcgatcga tagccacatc
     1801 cagccagcgc caggagtacc gccatacaaa tgatcgccct catcagactc ctccagtcac
     1861 aatattcacc agcttcttcg gtaccacgat caccttccgg gtcggtctgt ccgcaagcca
     1921 cgtcttaact cgctcgtccg cgagggctgc ctcacgcatg gcgccatcgt cggcgcccgc
     1981 aggcatgaag agtcggctcc ggacctttcc gtccacctgg acgaccacca caacctcctc
     2041 tgccgtgatg acggccgggt cgtaggtcgg ccaggccgcg tgaaagatgc ttccgctacg
     2101 accgagtcgt tcccacagtt cctcgcacag atgcggcgca aagggtgaca ggagcagcag
     2161 aagggtctcg accgcatagc tgtaggcgaa gcgccgctca tcagcttcgt cggtagg
//
LOCUS       DATMXI010000233         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_218051, whole genome shotgun sequence.
ACCESSION   DATMXI010000233 DATMXI010000000
VERSION     DATMXI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218051"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..990)
                     /locus_tag="VN444_04380"
     CDS             complement(82..990)
                     /locus_tag="VN444_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWR21082.1"
                     /translation="MRSYLRRQLFMAGCLIVLLSVSGCATLDLFSPKQAETPAGSDRE
                     LMSQAEAAIALKEYADGRKHLQRLINQFPESELVPTARLSVGRAYFDEKRYDEARAEY
                     QRFIELFPQHEQLDEAQYYTALSYFRQVEKVDRDQTMTRKAVRELRTLISEFRSSPFV
                     PDAQAMLAESQRQLAHRELFVGKFYFHREAYGAAISRFESVLKEHQGSEYDDQALYYL
                     GESLWELEQKEPAKAAFQRLIAEFPDSDMAPQAAKRIGVAFVQNPRSRKPPTSFLSTV
                     SATISDTMSELKNAILDSSIWAPALP"
     gene            complement(1146..2393)
                     /gene="clpX"
                     /locus_tag="VN444_04385"
     CDS             complement(1146..2393)
                     /gene="clpX"
                     /locus_tag="VN444_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWR21083.1"
                     /translation="MAKAVKGGGKLTCSFCGKSQDDVRKLIAGPTVYICDECIELCND
                     IIAEEWEEEKGKGFGELPKPIEIKSVLDQYVIGQDRAKKSLAVAVHNHYKRINTRMAF
                     DDVELTKSNILLIGPTGCGKTLLAQTLAKILDVPFTIADATTLTEAGYVGEDVENIIL
                     RLLQVADYDVERAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPQQEYLQVDTTNILFICGGAFVGLEKVIEQRIGQKSMGFGAEIQGRRGK
                     RTSESLANSQPEDLLRYGLIPELVGRLPVVAVVHELDEKALVRILVEPKNALLRQYQR
                     FFDFEHVKLTLTDEAISAVAQEAVKRQTGARGLRAILEEIMLDIMYEVPSQSGIAECI
                     VNRESVVERKPPIVLYKKKAESA"
     gene            complement(2498..>2620)
                     /locus_tag="VN444_04390"
     CDS             complement(2498..>2620)
                     /locus_tag="VN444_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWR21084.1"
                     /translation="EKDSDRDFFMSPEEAKEYGLIDEVIHSRVALPFSTTAPAS"
BASE COUNT          528 a          754 c          730 g          608 t
ORIGIN      
        1 gaagtaaggt aggggcgggg tttatccccg cccgataaac gggagggcat aaagccctcc
       61 cctacgaacg catcgccatt gctatggtaa cgcgggtgcc cagatcgaac tatccaggat
      121 agcgttcttc aactctgaca tggtatcgga tatcgtggcg ctgacggtgc ttaggaagct
      181 agtagggggc ttacggctac gcggattctg gacgaaggcg acgccgatcc gtttcgcggc
      241 ctgcggagcc atgtcggaat ccggaaactc tgcgatgagg cgctgaaagg cagccttggc
      301 cggttccttc tgctcgagtt cccagagcga ctctccaaga tagtacaggg cctggtcgtc
      361 gtactcggat ccctggtgct ccttcaggac cgactcgaac cgcgagatgg cggcgccata
      421 tgcctcacgg tgaaagtaaa acttgccgac gaatagctcc ctatgggcca gttgacgttg
      481 gctctctgcc aacatggcct gcgcgtctgg tacgaacggg gagctacgga attcgctgat
      541 cagggtccga agctccctca ccgcctttcg ggtcattgtt tgatcacggt ccaccttctc
      601 tacctggcgg aagtaagaaa gtgccgtgta atactgggct tcatcaagtt gctcatgttg
      661 tgggaacagt tcaatgaacc gttggtactc ggcgcgcgct tcatcgtatc tcttctcatc
      721 gaagtaggca cgaccaacgc tcaggcgagc tgtcggaacc aattcgcttt ctggaaattg
      781 gttgatcagg cgctgtaggt gtttgcgtcc atcagcatac tccttcaggg cgatggccgc
      841 ctcagcctgg ctcatcaact cccggtccga gccggctggt gtctcggcct gcttcggtga
      901 aaagaggtcg agagtcgcgc accccgagac ggagaggagg acaatcaggc aacccgccat
      961 gaagagttga cgccgcagat aggatctcat atcgagcacc tctgagctgt tcgagaaaca
     1021 ctcgttcctc tcgtcatgca gcgaacaaaa gactatcgag gagcgataga attacagact
     1081 tcggattgcc ggatggcaaa acaaggagag gaggaggagc cgtcaatccg taatgggttt
     1141 ggcgatcagg ctgattcggc ttttttctta tagaggacga tgggcggttt ccgttcaacc
     1201 acggattcac ggttgacgat gcactccgca atccccgact gtgaggggac ctcatacatg
     1261 atatccagca tgatctcttc gaggatcgcc cggagcccac gggcgccggt ctgccgtttt
     1321 accgcctcct gagccacagc gctgatcgcc tcgtcggtaa gggtcaattt cacgtgctcg
     1381 aaatcgaaga acctttgata ctgccgtaaa agggcattct ttggttctac aaggatccgc
     1441 accagcgcct tctcgtcaag ctcgtgtacg accgccacca ccggcagtcg ccccaccaac
     1501 tcagggatga ggccataccg aagcaggtct tccggttggg aattggccag tgactcactg
     1561 gtccgctttc cccgtcttcc ctggatctcg gcgccgaatc ccatcgactt ctggccaatc
     1621 cgctgttcaa tgaccttctc tagcccaacg aatgctccgc cgcagataaa caggatgttc
     1681 gttgtgtcaa cctgcagata ctcctgttgg ggatgcttgc gcccgccttg cggcgggaca
     1741 ttggccacgg ttccctcgag gatcttcagg agggcctgtt ggacgccctc tccggagacg
     1801 tcccgcgtga tggatgggtt ttcagacttg cgcgcgatct tgtcgatttc gtcgatgtaa
     1861 atgatccctc gctctgcccg ctccacatcg tagtctgcga cctgcagcag tcgaagaatg
     1921 atgttctcca cgtcctcccc gacatatccc gcctcagtga gggtggtggc atcggcgata
     1981 gtgaacggca cgtccaggat cttagccagg gtttgcgcca gcagggtctt gccgcatccg
     2041 gttggaccga tgaggagaat atttgacttg gtcagttcga cgtcgtcaaa ggccatccgg
     2101 gtgttgatac gcttgtagtg attgtggacg gcgactgcca gactcttctt ggcccggtcc
     2161 tggccgatga catactgatc caggaccgac ttaatctcga taggcttggg gagctctccg
     2221 aaacccttac ccttttcctc ttcccattcc tcagcgatga tgtcgttgca gagctcaatg
     2281 cactcgtcgc agatgtagac tgtgggaccg gcgatcagct tccgaacgtc gtcctgactt
     2341 tttccgcaaa aggagcaggt gagctttccc ccgcctttca ccgctttggc catctacccc
     2401 tccctctagc gttccaggtg tcgagttcga cgtcccaagt gaaatcttcg ccacgcgctg
     2461 gtctttgctt tggccatata cctgaaatac acaaccatta tgacgctggg gctgtggtcg
     2521 agaagggcag ggccacacgc gagtggatca cctcgtcaat caggccgtat tccttggcct
     2581 cctcaggtga catgaaaaag tcgcgatcgc tgtctttctc
//
LOCUS       DATMXI010000234         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_233350, whole genome shotgun sequence.
ACCESSION   DATMXI010000234 DATMXI010000000
VERSION     DATMXI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233350"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VN444_04395"
     CDS             <1..806
                     /locus_tag="VN444_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWR21085.1"
                     /translation="LANEVLQERQRPRADIFIATEAGVCEILAKEGLLEPYASLSART
                     IPAAHKSARGLWTGISGRARVIIYNKNLVRENDIPSSIFDLTDAKWKGKIAIAGTRER
                     TTLAWLSALVEVMGEGKAKAYIDRLIENGLKVLPDNSDVWRGVGSGEFAVGLTNSPNY
                     HLALEARLPVGVVYPDQGPKGIGVLVNPNAVAIVKGTKNLDQARQFVDFLLSKPAQEL
                     LTHHAFEIPLLPGIDPGQVRPLAGFKALQISQERLAGLEEKTITIFPGL"
     gene            862..2484
                     /locus_tag="VN444_04400"
     CDS             862..2484
                     /locus_tag="VN444_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWR21086.1"
                     /translation="MRRVELRQLATLWRSQAVGLQPAAIAGAALIILLTASPLVNLLT
                     SAFAGGLKAFSILMNSSMLSLIAETMLLATLATLWAMVLGLPPAWILTRTDLPGRRVL
                     QAVGVLPLAIPPYIGAFTYITLLGPVGWVNTVYQAIGGSGPIVNIYGLWGGALVLGLF
                     TYPYILLIVGSALKGSDPALEEAARAAGLGPVGIFLRVTFPLLRPSLLAGALLVFLYA
                     LSDFGAVSLLRVNTFTTEIFHQLNTRFDQRNAAVLSAILVFLTAVVLMAQRKSLGRRS
                     FVQQRSGSRPATIYPLGRWKALVLCCTYLILGGSVFLPVALLLYQTGSLMSLLHTLIA
                     GYRYLWNSLWTAAVAATVASGLGLFVAYLTQRRKGSAGLLLTSVTQLGYAIPGTVLGL
                     SLILLYNAYLPWIYGTAAMVILGYLLRFLPQAVQSSTTALAQVNPNLEEAARSLGRSP
                     WQALREVTVPLIRPGIAAGWMLVFISSMKELAATLLLRPAGFDTLPVRIWIASIEVDY
                     AGAAATSLILIVITALPLFLISRLDATVSQLN"
     gene            2493..>3404
                     /locus_tag="VN444_04405"
     CDS             2493..>3404
                     /locus_tag="VN444_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR21087.1"
                     /translation="MGNLAKKRIGEIAAAGTNIISWKQVSSPRPMVIRLDRVSKHYGG
                     DGTPAVEEISFSVEQGEILVLLGPSGCGKTTTLRLIAGFEAPDGGRIEIGGRTVAHST
                     IFLPPEQRGVGMVFQDYALFPHLTVQENVAFGLRHVSVEQQQNRIAEALELVGLSTLQ
                     GRYPYELSGGQQQRVALARALAPGPQVVLLDEPFSNLDADMRAQIREDVSAILRQAGT
                     TAIFVTHDQEEAFVIADRVGVLNHGRLEQLDHSEAIYHTPATRFIAGFVGSADFVPGL
                     VQGERIITELGTFPNQQELSVGQAIEMM"
BASE COUNT          632 a         1055 c         1011 g          706 t
ORIGIN      
        1 gcctcgcgaa tgaggtgctc caggaacggc agcggccgag ggccgatatc tttatcgcca
       61 ccgaggccgg tgtctgcgag atccttgcaa aggaagggct cctggagccg tatgcctctc
      121 taagcgcccg tacgattcca gccgcgcaca agagcgctcg tgggctctgg accggtatct
      181 ccgggcgtgc cagggtcatc atttacaaca agaacctcgt aagggagaac gatatcccaa
      241 gctcgatatt cgacctgact gacgccaagt ggaaggggaa gatcgccatc gccggtacgc
      301 gcgagcgaac gaccctcgcc tggctgagcg ccttggtgga ggtcatgggt gagggaaagg
      361 ccaaggccta catcgatagg ctgatcgaga acggtctgaa ggtcctacct gacaactccg
      421 atgtctggcg cggcgtaggg agcggtgagt tcgccgtggg attgaccaac tcacccaact
      481 accacttggc ccttgaagcc agactaccgg ttggggttgt ctatccggac caggggccaa
      541 aagggatagg cgttttggtc aacccgaatg ctgtggcaat cgtaaagggt accaagaacc
      601 tcgatcaggc caggcaattc gtcgatttcc tcttaagcaa gccggcccag gaactcctga
      661 cccaccacgc cttcgagatc ccgctgcttc ctgggataga ccccggccag gttcggccgc
      721 tcgcggggtt caaggcgctc cagatcagtc aagagcgtct ggctggcctt gaggagaaaa
      781 ctatcactat cttccccggt ctgtaaggtc cggcccaaca ccgaacaaca tggtgaagaa
      841 cgaggcatgg taagcagcaa cgtgcgacgc gtagaactta ggcagctagc cacgctgtgg
      901 cggtcgcagg cggtggggct tcagcctgca gccattgccg gggcggccct gattatcctt
      961 ctgacggcct ctcccttggt caatctttta acctctgcct tcgccggagg gctgaaggct
     1021 ttttcgatcc tcatgaacag ctccatgctc tcgctgatcg cagagacgat gttgctcgcc
     1081 accctcgcca cgctgtgggc tatggtcctc gggctgccac cagcctggat cctcacccgt
     1141 acggacctcc ctggacggcg tgtccttcag gccgtggggg tcctgccgct ggcgattccc
     1201 ccatacatcg gcgccttcac gtacatcacg ctgctcggtc ccgtcggctg ggtcaacacg
     1261 gtgtaccagg ccataggtgg tagcgggcca atcgtgaaca tttatggcct ctggggtggg
     1321 gccttggtgc tcggcctgtt tacctatccg tacatcctcc tcattgtcgg tagcgcgtta
     1381 aaggggagcg atcctgcgct cgaagaagca gcccgagccg ccggccttgg gccggtcggc
     1441 atcttccttc gggtcacgtt tcccctgctg cgccctagcc tcctggccgg ggcgctgctg
     1501 gtcttcctct acgccctctc ggacttcggc gctgtctcgt tgctccgggt caacaccttt
     1561 accaccgaga tcttccatca actcaataca cgctttgacc agcggaatgc ggccgtcctc
     1621 tccgctatcc tggtattcct cacggccgtg gttctgatgg cccagcggaa aagcctgggc
     1681 cgtcgcagtt ttgtccagca acgaagcggt tccaggccgg ccaccatcta cccacttgga
     1741 cgatggaagg cgctggttct gtgctgcacc taccttatcc tcggcggctc ggtctttctg
     1801 ccggttgcgc tactgctcta ccaaaccggc tcactgatga gcctcctgca cacactcatt
     1861 gccggctacc gctatctgtg gaacagtctc tggacagcag ccgtggcggc tacggtggca
     1921 tcgggacttg ggctcttcgt cgcctacctt acccagcgcc gaaaagggtc ggcaggactc
     1981 ctcctcacgt ccgtcaccca actcgggtac gccatccccg gcacagtcct tgggctgagt
     2041 ctgatcctcc tctacaatgc gtacctaccc tggatctacg ggaccgccgc catggtgatc
     2101 cttggctacc tcttgcggtt tctcccccaa gcagtccaga gcagtacaac cgcgctggct
     2161 caggtcaatc cgaacctgga ggaggcggcc cgtagtcttg ggcggtctcc ctggcaggcg
     2221 cttcgtgagg ttaccgttcc cctcatccga ccggggatcg cagcgggatg gatgctcgtc
     2281 tttatctcgt cgatgaagga actggcggcc acgcttctcc tccgcccggc tggattcgac
     2341 accctgccgg taagaatttg gatcgcctcc attgaggtcg attacgccgg ggccgcggcg
     2401 acctcactga tacttatcgt catcaccgcg ctccccctct tcctcatcag taggcttgac
     2461 gccaccgtat cacagctcaa ttgagtgcag tgatgggcaa cttagcgaaa aagaggatcg
     2521 gagagatcgc ggcagcaggg acgaatatta tctcgtggaa acaggtctca tcaccgcgcc
     2581 ccatggtcat ccggctagat cgggtgagta agcactacgg aggagacggt acccccgcgg
     2641 tcgaggagat ctccttctcc gtcgaacaag gagagatcct ggtgttgctt ggacccagcg
     2701 gctgcggaaa gaccacgacg ctacgcctca tcgccgggtt tgaggccccc gatggcgggc
     2761 ggattgagat tggcggccga acggtagccc acagcacgat cttcctgccg ccggagcagc
     2821 ggggcgtggg aatggtcttc caggactatg cgcttttccc gcacctgaca gtccaggaaa
     2881 acgtcgcatt cggcctgcgc catgtgagtg tagagcagca gcagaaccga atcgcggagg
     2941 cgttggaatt ggtagggctc tcaacgcttc aggggcggta cccatatgag ctctccggcg
     3001 ggcagcagca acgtgttgct ctagcgcgcg cgctggcgcc aggcccgcag gtggtcttat
     3061 tggatgagcc gttcagtaat ctggatgccg atatgcgagc gcagatacgg gaagatgtca
     3121 gtgcgatcct ccgccaagcc gggaccactg ccattttcgt gacccatgat caggaggagg
     3181 catttgtcat cgctgatcga gtcggggtgc tgaaccatgg gcgcctggag cagctcgacc
     3241 attcggaggc gatctatcat acgccggcta cccgttttat tgccgggttc gtcggttcgg
     3301 ccgatttcgt ccccggcctc gtccaaggcg agagaattat tacggagctg ggcacgttcc
     3361 cgaaccagca ggagctttcg gtagggcaag ccatagagat gatg
//
LOCUS       DATMXI010000235         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_116150, whole genome shotgun sequence.
ACCESSION   DATMXI010000235 DATMXI010000000
VERSION     DATMXI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116150"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VN444_04410"
     CDS             complement(<1..722)
                     /locus_tag="VN444_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HWR21088.1"
                     /translation="MAGRRRENTSVPLTAKAQLSAATKRARDIMRKDAGLNGDLDRIP
                     QLSWIMFLKCFDDLEQRREVTEKKYRLAIEPPYRWRDWASDPDRGLTGESLLKFINDE
                     MFPYLRSLTGTVAGDARDVLAAVFKETYNRMLSGYLLRDVVNLVNRLNFNSSDDIHTL
                     AHLYESMLREMRDAAGDSGEFYTPRPVIRLIVQQINPRLGETVLDPAAGTGGFLVETY
                     EHLKPQVKSVEDRRRLQEETIFG"
     gene            complement(732..1595)
                     /locus_tag="VN444_04415"
     CDS             complement(732..1595)
                     /locus_tag="VN444_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HWR21089.1"
                     /translation="MRYANAAAFRMALEERIRQRSAADGVAIQRLRKRVTFERFLSRL
                     QAPPDSPWFLKGAFALDLRFGDRSRTTKDLDLAVDLAIAPNVPTTPAGVAERLQAAAA
                     YELDDFIGFSVSAEGEQIQQEPSARTYRFHVRALLDRRPFEDFKVDVGTVTPLIAPTE
                     EIPESDTLLFAGIVPRRFRAISLAQHFAEKIHAITFPREDWEKTRVKDFVDIVLILEV
                     NAPDPGTARDALQAVFDGRGTHSLPSQISDPPPSWTGSYTATARELTLTHATIEDGTR
                     LLHQFWAKVFP"
     gene            complement(1595..2200)
                     /locus_tag="VN444_04420"
     CDS             complement(1595..2200)
                     /locus_tag="VN444_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HWR21090.1"
                     /translation="MGKRDPQEARRRLYEYVVAQGGYFTATQARIAGYPKQLQYYHVR
                     RGNWVREDRGIFRLWEWPRSSYEDLIRWTLWSRGAAVVSHQSAMAVHEISDLMPAKIH
                     MTVPPGFRKTPSPVMVLHRDRLAPHEIEQRDGFRVTTPLRTLIDAARVDVDPERLSAA
                     VRDAILKGLVADRHIDAAIETLLGAAAERLYQALIVARRAA"
     gene            complement(2300..3358)
                     /locus_tag="VN444_04425"
     CDS             complement(2300..3358)
                     /locus_tag="VN444_04425"
                     /inference="COORDINATES: protein motif:HMM:NF020052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HWR21091.1"
                     /translation="MRAALHKANEDLAQQYPHYVARVTADEGKVGRGHLDDFVDPERD
                     MPVILTTSKLLSTGVDAPTCRNIVLFKPIGSIVEFKQIIGRGTRLYPDKDKLWFTILD
                     YAGATRLFADPNFDGEPERTTEERIYPSSEEQGPPAMVGEGPSHGREEDEDVPVGDWQ
                     EQLHRKYYVDGVEVRITAEVVYELDSSGKKQRVVKYTDYAAEQVRRLCPTPAELRTEW
                     MDAEHRGAIQQALSERGIDFSTLVEATHQPDADPFDLLIHVAWNAPLRTRRERVERVR
                     RERKDFWDLYTPQARAVLNDLLDKYTDYGVTQLDDLQILEVPPLSERGTPMEIAGFFG
                     GVDHLRSAVAELQSILYK"
     gene            complement(3374..>4406)
                     /locus_tag="VN444_04430"
     CDS             complement(3374..>4406)
                     /locus_tag="VN444_04430"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWR21092.1"
                     /translation="QQAMEYAEILGLRFAYSTNGHGIVEHDYTTGRQQVLDIFPSPDE
                     LWRRLRAAEGLADDKAAEDLLFPFNRELRNPDGSIKTPRYFQEIAIHRAVQAVLQGKP
                     RILITMATGTGKTFVAVQIVWKLWKTGHKARILYLVDRNILVDQPLVREFNIFGDAVW
                     KIQGEAKKGREIYFALYQALAEDESRLGLYKEYPPDYFDLIIVDECHRGSATDQSRWR
                     RILEYFDPATQIGMTATPLREETRDTYRYFGNPIYTYGLSQGIEDGFLAPYRVHRIVP
                     SIDATGWQPEQGQLDRFGREIPNGVYETKDFERVVSLLSRTEVVAQHLTAYLKRTDRF
                     AKTMVFCVD"
BASE COUNT          897 a         1263 c         1246 g         1000 t
ORIGIN      
        1 ccgaagatcg tttcttcctg gaggcgccgc cggtcctcca cgcttttcac ctggggcttt
       61 aggtgttcat aggtctctac gaggaagccg ccggttcctg ctgccgggtc cagcaccgtc
      121 tctcccaggc gagggtttat ctgctgaaca ataagccgaa tgacgggccg gggtgtgtag
      181 aactcgccag agtcccccgc cgcgtctcgc atttcgcgaa gcatagactc gtacaagtgg
      241 gccagcgtgt ggatgtcgtc agaagaatta aaattaaggc ggttgaccag gttcacgaca
      301 tcccggagga ggtatccgga aagcattcgg ttataggttt ccttgaagac ggccgccaga
      361 acgtctcgcg catcccctgc cacagtcccc gtaaggctcc gcagatatgg gaacatctcg
      421 tcgttgatga acttgagaag cgactctcct gtcagtcccc tgtccgggtc gcttgcccag
      481 tcgcgccacc ggtaaggtgg ctcgattgcg agccgatact tcttctccgt cacctcgcgc
      541 cgctgttcaa ggtcgtcaaa gcattttaag aacatgatcc aggagagctg gggaatacga
      601 tccaggtcac cgttcaatcc ggcatccttg cgcatgatgt cccgtgctct cttggtggcc
      661 gctgacagtt gtgcctttgc tgtcagcggc actgaggtat tttctcttct gcgccctgcc
      721 atactctcac ctcacggaaa aactttggcc caaaattgat gaagaagcct ggtgccatct
      781 tctatggtcg cgtgagtgag tgtgagctcc cttgcagtgg cggtatatga cccagtccat
      841 gagggcggcg gatcggaaat ctgagaggga agagaatgcg tgcctcttcc atcgaacacc
      901 gcttggaggg catcccgggc tgtgccggga tcaggtgcgt taacctcgag gatcagcaca
      961 atgtccacaa aatccttcac cctggttttt tcccaatctt cccgcggaaa ggtgatcgcg
     1021 tgaatctttt cggcaaagtg ctgagcgagg gagatcgctc ggaaccgtcg tgggacaatc
     1081 cctgcaaaga gcaacgtgtc gctctcagga atctcttccg tgggtgcaat cagtggcgtt
     1141 acggtaccga catcgacctt gaagtcttcg aaaggacgtc tatccaggag tgcccggacg
     1201 tggaaccgat aggtgcgagc gcttggttct tgctggatct gttctccttc agccgagaca
     1261 ctaaaaccga tgaagtcgtc tagctcgtat gctgcggccg cctgcagacg ctccgcgacc
     1321 cccgctgggg tcgtggggac attgggcgcg attgcaaggt caactgcgag gtcgaggtct
     1381 ttcgtggtgc gggatcgatc tccaaatcga agatccaggg cgaatgcccc tttcaagaac
     1441 caagggctgt ccggtggggc ttgaagacga ctcagaaatc gctcgaatgt gacgcgcttt
     1501 ctgagtcgtt ggatcgccac tccatcggcg gcactacgct gtctgatgcg ctcctccagc
     1561 gccatccgga aggctgccgc attggcgtat ctcatcaagc agccctccga gccacgatga
     1621 gcgcctgata caggcgttct gccgccgctc cgaggagtgt ctctatggca gcgtcgatgt
     1681 ggcgatctgc aacgaggcct ttcaaaatgg catcccggac cgccgcggat agacgctcag
     1741 ggtctacatc gacgcgggcg gcatcaatca aagtcctaag aggtgtcgta acacgaaacc
     1801 cgtcacgctg ttcgatttcg tggggagcaa gacggtcccg atgaaggacc atgactgggg
     1861 atggggtttt gcgaaaccct ggcggaaccg tcatgtgtat tttggccggc atcagatcgc
     1921 tgatctcatg caccgccatc gcgctctgat gggaaaccac cgctgcgccc cgactccaca
     1981 acgtccagcg aatgaggtct tcgtatgagc ttctcggcca ttcccagagg cgaaagatac
     2041 cacggtcttc tcgaacccag ttgccacgcc tgacatggta gtattggagc tgcttgggat
     2101 agccggctat cctagcctgg gtagccgtaa aatagccgcc ctgagcaact acatattcgt
     2161 aaagacgacg cctggcctct tgtggatctc gctttcccat tgtgacaata aagtaccatt
     2221 cgatatgacc tttatcaata ataatatgat gataatcata taaggtaata ttctatttta
     2281 cttaactgta tcactacgtc tatttgtaca agatggactg aagttcagcc actgcagatc
     2341 gcaggtgatc gactcctcca aagaaaccgg caatttccat aggagttccg cgctcggaga
     2401 ggggtggaac ttcaagaatc tggagatcat cgagctgcgt gacaccgtaa tctgtgtatt
     2461 tatccagcag atcattgaga acagcgcggg cctgaggtgt gtacaggtcc caaaagtcct
     2521 tcctctccct ccggacacgc tcgacgcgct cccgcctggt tcggagggga gcgttccacg
     2581 ccacatggat gagaagatcg aacgggtcgg catccggctg gtgtgtggcc tcgaccaggg
     2641 tggagaagtc aatgccgcgt tcggagaggg cctgctgaat cgccccccgg tgttctgcgt
     2701 ccatccactc tgtccgaagt tcagcggggg tggggcacag ccggcgcact tgctcagccg
     2761 catagtccgt gtacttgacg actcgttgct tcttccctga actatctaac tcatacacca
     2821 cctctgcggt aatccgcacc tcgaccccat ccacgtagta cttcctatgc agttgctcct
     2881 gccagtcgcc cacgggcacg tcttcgtctt cttctcgtcc atgcgaaggg ccttctccga
     2941 ccatcgcggg tggcccttgc tcctcgctag atgggtaaat cctttcctca gtagtccgtt
     3001 caggctcccc atcaaagttg ggatcggcga ataagcgggt agccccggcg tagtccagga
     3061 tagtaaacca aagcttatcc ttgtccggat agaggcgggt gcctcgaccg atgatctgct
     3121 tgaactccac gatggagccg ataggcttaa acaggacaat gtttcggcac gtgggggcgt
     3181 ccactccggt gctgaggagc ttagatgtcg tcaggatcac cggcatgtcg cgctcgggat
     3241 ctacgaagtc atccagatgg ccccgaccca ccttcccttc gtcggctgtt acgcgtgcca
     3301 cgtagtgtgg atattgctgg gcgaggtctt cattggcctt gtgcagcgcg gcacgcatgt
     3361 cttcggcgcg ctcttagtcc acgcaaaaca ccatggtctt ggcgaatcgg tctgttcttt
     3421 tgagataggc ggtcagatgc tgggccacga cctcggtccg actgagaagg gagaccacgc
     3481 gctcgaagtc cttggtctcg tacacgccgt tcggaatctc ccgcccgaag cggtcaagtt
     3541 ggccctgctc tggctgccac ccggtggcgt ctatgctggg aacgatgcgg tgcacgcgat
     3601 agggagccag gaacccatcc tcgataccct gactcaagcc gtaggtatag atggggttgc
     3661 cgaagtaccg gtaggtatcc cgcgtctctt cgcgcaaagg cgtagccgtc atcccgatct
     3721 gagtggccgg gtcaaagtac tctaggattc ggcgccagcg ggactgatcc gtcgcgctcc
     3781 cacggtggca ctcgtccacg atgatcaggt cgaagtaatc cggcgggtat tctttataga
     3841 ggcccagccg cgactcatcc tccgccaggg cctgatagag cgcgaagtag atctctcgtc
     3901 ccttcttcgc ctctccttgg atcttccaga cggcatcacc gaagatgttg aactcgcgaa
     3961 ccagaggctg atccacgagg atgtttcggt caaccagata cagtatccgc gctttgtgcc
     4021 ccgttttcca gagcttccac acgatctgaa cggcaacaaa ggttttgccg gtccccgtgg
     4081 ccatggtgat caggatgcga ggtttgcctt ggaggacggc ctgcacagcg cgatggatgg
     4141 caatctcctg aaaataccgt ggggttttga tggaaccgtc cggattgcga agttcgcggt
     4201 taaacggaaa caacaggtct tcggctgcct tgtcgtcggc caggccttcg gccgcacgaa
     4261 gccgacgcca cagttcatca ggagagggaa atatgtccag gacctgctgc ctacctgtgg
     4321 tgtagtcgtg ctctacgatt ccatggccgt tcgtcgaata ggcaaatcta agcccgagga
     4381 tttccgcata ctccattgcc tgctga
//
LOCUS       DATMXI010000236         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_75087, whole genome shotgun sequence.
ACCESSION   DATMXI010000236 DATMXI010000000
VERSION     DATMXI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75087"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            552..1547
                     /locus_tag="VN444_04435"
     CDS             552..1547
                     /locus_tag="VN444_04435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWR21093.1"
                     /translation="MSRIALITGLTGQDGSYLAELLIDRGYDVHGIVRRVALEHPDHR
                     LWRIRHLLDRVHLHAASLDSLSSLYRVFHAVQPEECYHLAAQSFVATSFEDEFSTMQT
                     NITGTHHVLSALKDCAPQCRVYFAASSEMFGQVASSPQDESTPFHPRSPYGISKVAGF
                     DLTRYYREAHHLHASSGILYNHESPRRGLEFVTRKITSTVARIHIGLTDQLELGNLDA
                     RRDWGHSRDYVQAMHLMLQQDTPDDYVIATGEQHSVLDFVREAFRCVGIDWQEAMQYV
                     TINPAFFRPADVETLCGDASKAKRILGWKPTTSFTQFVREMVESDIAYYVTQQRS"
     gene            1549..1755
                     /locus_tag="VN444_04440"
     CDS             1549..1755
                     /locus_tag="VN444_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21094.1"
                     /translation="MLMKVAGVWRQRLAITDIGEDLSTCLTPEENARFSAFLEQYEGD
                     VETRKRVRFAGHTAHRRAKKGEGT"
     gene            1752..2138
                     /locus_tag="VN444_04445"
     CDS             1752..2138
                     /locus_tag="VN444_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21095.1"
                     /translation="MTRYGTLAVLVLFTAACALPLNQQYRPIMDLKGRDDHTAAYDQF
                     QCHQLAEQAALQNNIGADAAGGLVAGAALGALLGAVSGHAGSGAAYGSILGVAAGAGS
                     SAGDQLSTYKHALRKCLAGRGYSVLN"
     gene            2233..2598
                     /locus_tag="VN444_04450"
     CDS             2233..2598
                     /locus_tag="VN444_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR21096.1"
                     /translation="MSRAISDAGWAEFGRMLAYKTQWYGSELRVIDRFAPSSKTCSDC
                     GAINKALRLSDRVWVCEACGCIHDRDDNASINIREFGLNDTGSHRPESEASASRACGD
                     RISKRRSMKQEASTPRCQR"
     gene            2884..3336
                     /locus_tag="VN444_04455"
                     /pseudo
     CDS             2884..3336
                     /locus_tag="VN444_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
     gene            3341..3922
                     /locus_tag="VN444_04460"
     CDS             3341..3922
                     /locus_tag="VN444_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21097.1"
                     /translation="MANNRIAIDRVIVETLYLAEKKSLSEVGGILGCNTATIRNRMKE
                     WGIPRRTVAVSLKNRKHTWGDKISAALQGRVISQEMRDKISSTRLGLNLPAWNKGLRK
                     ATHPQLITYGCVGPDHWAWQGGISAENALLRQSSEYKQWRDEVFHRDDFTCQFCERKG
                     GYLEAHHIISMAVLLKCEVTRVRVKPLPLGMGR"
     gene            3974..>4890
                     /locus_tag="VN444_04465"
     CDS             3974..>4890
                     /locus_tag="VN444_04465"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR21098.1"
                     /translation="MKLIINLKLKPTIAQQKTLKETLERCNAACNWLSDQAFKSKVFG
                     QYALQKAHYYAIREKFGLTAQASIRCLAKVADAYKLKNRRTVKREFRNWAAQPYDDRI
                     FRFAKGDTVNLWTLDGRQTIPFQCGKYQRDLIPFRKGEVDLMFIKKKWYVACIVDVDE
                     AEPITPKGVLGIDLGIVNIASDSTGESFSGKQVEEIRSHYAKRRATLQSIGTKAAKRR
                     LKSMSGKQERFQKITNHTISKAIVSKAKRLQLSIAVEDLTGIRSSVKAKKAQRGKLHN
                     WSFRDLRSKIEYKAKRQGIPCVAIDPRNT"
BASE COUNT         1195 a         1303 c         1316 g         1076 t
ORIGIN      
        1 atattattgc ctatcaagac ggaccaataa aatcaagcac ttgcaaatcc atgccaaggt
       61 tgttggcatg agacttgccg aggaaggaaa aattgttgag agggggaggt ggttttaagg
      121 ggtctcccta ttctcccccg ctgtcacatt ttccactctt gcaacaaatc tcttctcgta
      181 tcttcgcaaa aacgctctct gtacgcacat cacccttgcc aaaacatacc agatatagta
      241 cattatatac tatatgtagg acttttcgtt gaaaggagca agcaagatgt ttccctgccc
      301 tcagtgtaaa cacacgaaaa ccacagttgt aacgacgagg aatcggcagc accggcccgg
      361 cattgtccgg tatcgccggt gtccgacatg tcgagcgaca ttcaagacac tcgaactcct
      421 cgtcgagaag aaggagcaac atgaggaacc gcgcagaaca tgagagaata gtgcaagatt
      481 ttacttgaca cgtaatttgt tgcgctgtat attgctgtag cggtatcact cacacacaaa
      541 aaggagtgtc aatgtcgcgc atcgcgctga ttaccggcct aaccggccaa gatggcagct
      601 accttgccga actcctgatt gatcgtggct atgatgtcca cgggattgtc cgccgtgtcg
      661 ccttagaaca tcccgatcat cgtctctggc ggattcggca cctcctcgac cgagtacatc
      721 ttcatgccgc ctcgctcgac agcctctcta gtctctaccg ggtgttccac gccgtgcagc
      781 ctgaggagtg ctatcacctc gcggcccagt cgtttgtcgc gacctccttc gaggatgaat
      841 tctcgacgat gcagacgaac atcaccggca cccatcacgt cctgtcggcg ctcaaggact
      901 gtgctccgca gtgccgagtc tacttcgccg caagttccga gatgttcggc caggtcgcct
      961 cgtcacccca ggacgaatcg acgccctttc acccccgttc cccttatggc atctcaaagg
     1021 tggcgggatt cgatctcacc cggtactacc gggaagctca tcacctccat gcgtcatcag
     1081 ggatattgta caatcacgaa tcgccccgtc gcggactcga atttgtgacc cgcaagatta
     1141 cctcaaccgt cgcgaggatt cacatcggcc tcaccgatca gcttgaactc ggcaatcttg
     1201 acgcacggcg tgactggggg catagtcggg actacgtgca ggccatgcac ctcatgcttc
     1261 aacaggacac cccagacgat tacgtgattg cgaccggcga acagcattcc gtcttagact
     1321 tcgttcgaga agcctttcgg tgtgtcggca tcgactggca ggaggcgatg caatacgtga
     1381 cgatcaatcc ggcgttcttc aggcccgccg acgtggagac cctctgtggc gatgcgtcaa
     1441 aagcgaagcg gatattggga tggaagccga caacatcatt cacacagttt gtgcgggaga
     1501 tggtggaatc tgacatcgcg tattacgtca cacagcaaag gagttgagat gctcatgaag
     1561 gtcgcaggcg tctggcgtca acgactggct ataaccgaca tcggagaaga cctgagcacc
     1621 tgcttgacac ctgaagagaa cgctcggttc agcgccttcc tcgaacaata cgaaggcgac
     1681 gttgagaccc gcaagcgcgt ccggtttgcc ggacatacgg cccaccgacg ggcgaagaaa
     1741 ggagaaggca catgacccgc tacggtacgc tcgcagtcct tgtactcttc accgcagcct
     1801 gtgcgctgcc cctcaaccag cagtatcgcc cgattatgga tttgaagggc cgcgatgacc
     1861 acacggcagc ctacgatcaa ttccagtgtc accaactcgc ggaacaggcg gcccttcaga
     1921 acaacatcgg ggccgatgcc gcaggaggac tcgttgccgg agccgcgctt ggggcactcc
     1981 tcggagccgt cagcggtcac gccgggtcag gagccgccta cgggtcgatt ctcggcgtcg
     2041 cagccggagc cggatcatcg gcaggcgatc aactgtctac atacaaacac gccctgcgca
     2101 aatgtcttgc cggacgcggg tattcggtgt tgaattagta cgcgaatcgc gcaacataat
     2161 cgttgacagc aacacgctaa cgtcgtagga tgcacatatc acgaagctgc tctatagcga
     2221 agacaagggt gtttgtcccg cgccatttcg gatgcggggt gggcggagtt cggtcggatg
     2281 cttgcgtata agactcagtg gtatgggtcg gaattgcgtg ttatcgaccg ctttgcgccg
     2341 agcagtaaga cgtgttctga ctgtggcgca atcaacaaag cattgcgact atcggatcgt
     2401 gtgtgggtct gcgaggcgtg tggttgcatc catgatcggg acgataacgc gagcatcaac
     2461 attagagagt ttggattgaa cgatacgggc agtcatcgcc cggaatcaga ggcttccgcc
     2521 tctcgcgcct gtggagatcg gatcagtaag cgccggtcga tgaagcagga agccagcaca
     2581 cctcgatgtc agaggtgata agtcttgcgg aacggttcga atcccgtcgc atcgtaagat
     2641 gtgtagctca aacggtagag cgcgaaaagc tcccttgtcg tttgttctta gagcatcatt
     2701 gctgcggatg gcgaaggcgg cggttacttc ttggatgaaa atgaccgccg tcgctggttc
     2761 ctccgcaatt cacagaaagg gtagcgtaga gttgagttgc ttcggttaga cggcgcaagc
     2821 cgtggggttt caacacccct acactcaatt cggatgttct ccctttcctt taaaggagac
     2881 agcatggcga agaccaacac aagacctgct cccatccaca ctcatgaagg cgcagtcgcc
     2941 tcccatatca atcccaccct tcaactccgc cggtcagtca tggcctgtct gctctgggaa
     3001 gatagttttt atgaagacgg ccagagcatt gccgaacgga tcattcagac tattcctaca
     3061 gtcgccccgg aggcggtcgc cgcaatggcg attgaggcca gagaacagat gaagcttcgg
     3121 catgtgccgc tcttgatggt ccgcgaaatg gcgaggctcg acacgcacaa acacctcgtc
     3181 gctccaacac tcgcccggat cattcagcga gcggacgaat tggctgaatt cctcgcgctg
     3241 tattggcggg acaagcgtca accgttgtcg gctcaggtca aacgggggct tgcggcggca
     3301 ttccagaaat ttaatcgtta tcaactgagc aagtatcgtc atggctaaca accggatcgc
     3361 catcgacaga gtcatagtgg aaaccctcta cctcgcagag aagaaatctt tatcagaggt
     3421 gggtggcatt ctgggatgca atacggcaac gattcggaat cggatgaaag aatggggcat
     3481 ccctcgcagg acggttgctg tgagtctgaa gaatcgtaag cacacatggg gagataaaat
     3541 aagcgcggcc ctgcaagggc gtgtaatatc gcaggagatg cgggataaaa tatcctccac
     3601 tcgcttggga ctcaatctcc ctgcttggaa taaaggactc cggaaggcca cacatccaca
     3661 actgatcacg tatgggtgtg tcggacctga tcactgggca tggcaaggcg ggatcagcgc
     3721 cgaaaatgcc ctactgagac agtcgtcgga atataagcaa tggcgcgacg aagtgtttca
     3781 tcgggatgat tttacgtgcc agttttgcga gagaaaaggc ggttatttgg aggcgcacca
     3841 tattatatct atggcggtgc tgttgaagtg tgaagtcacc agggttcgcg tgaagcccct
     3901 gcctttaggc atggggaggt aagcgaacgg tttttagctt gacttttatt caaattgcct
     3961 ctatacttga aatatgaaac tgattataaa tctgaagctc aaacctacta ttgcccagca
     4021 aaaaaccctc aaggagaccc ttgaacgctg caatgctgcg tgtaactggc tttccgacca
     4081 agcgtttaag tccaaggtct ttggacaata tgctttgcag aaagcgcatt actacgccat
     4141 tcgggagaag tttggcctta ccgcgcaagc ctccattcga tgccttgcaa aggttgctga
     4201 tgcctataag ctcaagaatc ggcgtaccgt aaagcgtgag tttcgcaatt gggcggctca
     4261 gccttacgat gatagaatct ttcgctttgc gaagggcgat accgtcaacc tctggacact
     4321 ggacggcagg cagacgattc cttttcagtg cggaaaatat cagcgcgacc ttatcccctt
     4381 ccgcaagggc gaggttgatc tcatgttcat caagaagaaa tggtatgtcg cctgtatcgt
     4441 tgacgttgac gaagctgaac cgattacccc gaaaggcgta cttggaattg atctgggaat
     4501 tgttaatatt gcatctgaca gcacaggcga atctttcagt ggcaagcagg ttgaagagat
     4561 tcgatctcat tacgcgaagc gccgcgcaac ccttcagagc ataggcacca aggctgcgaa
     4621 gcgtcgcttg aagtcgatga gtggcaaaca ggaacggttt cagaagatca cgaatcatac
     4681 catctctaaa gcaatcgtct ctaaggccaa acgcttgcag ttgagcatcg ccgttgaaga
     4741 cctgacgggc atacgctctt ctgttaaggc caagaaggcc caaagaggca aattgcacaa
     4801 ctggtctttc cgtgatcttc gctccaaaat tgagtacaag gccaagcggc aaggaattcc
     4861 gtgcgttgcc atagaccctc ggaataccag
//
LOCUS       DATMXI010000237         4921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_212341, whole genome shotgun sequence.
ACCESSION   DATMXI010000237 DATMXI010000000
VERSION     DATMXI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212341"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VN444_04470"
     CDS             complement(<1..398)
                     /locus_tag="VN444_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWR21099.1"
                     /translation="MITLKPAKGDIRAATPATTKKILTILEETYPDACVTLDFKSPFQ
                     LLIATILAAQCTDERVNQVTKGLFRRYPTPKSFADAEPVELEEAIRSTGFYRNKARSI
                     IGCCKKLVEEFGGQVPQTMEELITLSGVWR"
     gene            complement(395..1333)
                     /locus_tag="VN444_04475"
     CDS             complement(395..1333)
                     /locus_tag="VN444_04475"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWR21100.1"
                     /translation="MKTDLSVTVAGIRMQNPVMTASGTFGYAQEFEPFLDLSRLGAIV
                     VKTITRTPRAGNPPPRIVETPAGMLNAIGLQNVGVQAFIEEKLPYLRTLGPPVIVNIA
                     GESIEDYVELAKRLSDHEGISGFELNISCPNVADGLIFGCDATLANKLVACVRQATPL
                     PLIPKLSPNVTDAVEIARALADAGADALSLINTLIGMAIDVRSRRPKLGNVTGGLSGP
                     AIRPIAVRMVWEVARAVKLPLIGMGGIMTADDALEFLIAGATAVAVGTANFTSPTSTE
                     RVIDGIEAYMIEHRVKHMTDLIGSLDLTGTAREVTE"
     gene            complement(1330..2226)
                     /locus_tag="VN444_04480"
     CDS             complement(1330..2226)
                     /locus_tag="VN444_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWR21101.1"
                     /translation="MAVVYLNGRYVQAERAKVSAFDRGFSYGDGLFETIRAYTGWIFG
                     LERHLGRLKKGADQIGIPFEGDVGTWRKVMGELLRRNRLQDADASLRLTVTRGPDVMG
                     SLLPPDAPPSPTLLLVAKPLDAGIAERQQVGIGAVTIRWGSPFNPLRIKSLDYLYNML
                     AMAHAKREGAVEALFVGADGCVIEGTTSNLFSISQGMLTTPPEDSGLLPGITREVVIE
                     LARREGLIVHEAPVPLDELLATDEAFLTGSLKEITPLIAIDGCTIGAGSPGPVTRRLQ
                     QCYQAAAQEELTQGRRSMKGKA"
     gene            complement(2236..3702)
                     /gene="pabB"
                     /locus_tag="VN444_04485"
     CDS             complement(2236..3702)
                     /gene="pabB"
                     /locus_tag="VN444_04485"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWR21102.1"
                     /translation="MTLSFPTSMRGTSHTDPHLLIEEVSNPPALTDLLGLVAAEPGFS
                     LLDADPWAVNHWRSPCFGYDPFLTFSAKGDEITISLQGESRRVRGDPFQHLQAALRQY
                     GDVTSAGDVPIAGAIGYFGYDLRHHLERLPAVAVDDLRLPDCVIHFYDRLFWFEPANG
                     ELLVTSSGLPLPPGIARERRALERLQEGRDLIARAGLTGGSGPPELSHLETPLESNMS
                     KAQYCKALETVLEYIAAGDIYQANISQRFATQFSGEPWALYQRLRSRFPAPFGAYLHC
                     GPFHVLSNSPERFLLVEGNRISTCPIKGTRPRGMTVEDDARIIGHLRHDPKECAEHVM
                     IVDLERNDLGRICQVGSVHVEQFETIETYHTLHHMVSTVAGTLQDGTDPIDCLRATFP
                     GGSITGAPKIRAMEVIDEVEPTARGLYTGAIGFIGFSGGMELNIAIRTAIVTGRRIYF
                     QTGGGIVADSSPAREYEETLLKAETFFRTLGVSAGFGR"
     gene            complement(3699..4655)
                     /locus_tag="VN444_04490"
     CDS             complement(3699..4655)
                     /locus_tag="VN444_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWR21103.1"
                     /translation="MSPTECQVEVTANREIAPSYFSMRLAGPARLTRFRPGQFLMLGW
                     TDGRDPLLPRPMSIRRVREVQGSRFKVQGVEPQTRNPKPETRMAEVEILYKVCGKGTT
                     LLATTRPGRSLRLLGPLGNGFEIPRKVTSAVLIAGGIGVPPIAALAEALAARRAARGT
                     KMAVFLGGRSKADLLCVSDFRRIGATVHLTTEDGSLGHKGLVTNLLEQFLNTQYSALN
                     TRLYACGPHPMLSAIAPIAKKYNLPYQASMEANMACGFGACMGCVVPVKGDDPGRAYR
                     LVCKDGPVFDGHEVLWSNSSIPFVRSTDQINRIDQMDKMDRI"
     gene            complement(4725..>4921)
                     /locus_tag="VN444_04495"
     CDS             complement(4725..>4921)
                     /locus_tag="VN444_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21104.1"
                     /translation="IGLVINTPEGHHARLDSYSIRRTAVTMGIPYFTTMAAACAAVEA
                     IQAMRHGKLRVQALQEYHAE"
BASE COUNT          974 a         1518 c         1455 g          974 t
ORIGIN      
        1 ttgcgccaca ctccggacag cgtgatcaac tcctccatcg tctgcggcac ctgaccgccg
       61 aactcctcga ccagtttctt gcagcagccg atgatgctcc tggccttgtt gcggtaaaag
      121 cccgtcgaac ggatcgcctc ctccagctcc accggctcgg catcggcaaa cgacttcggc
      181 gtcggatacc gtctgaacag ccccttcgtg acctgattca cccgctcgtc ggtgcactgg
      241 gccgccagga tcgtggcgat cagcaattgg aacggactct tgaagtccag cgtcacacac
      301 gcatcagggt aggtctcctc aagaatggtg aggatctttt tcgtggtggc aggggtggca
      361 gcacgaatat cgccttttgc cggtttcaac gtaatcattc agtgacctct cgggcggtgc
      421 cggtcagatc tagggagccg atgagatcgg tcatatgttt aacccggtgc tcaatcatat
      481 acgcctcgat cccatcaatg actcgttccg tactggtcgg tgaggtgaag ttagccgttc
      541 caaccgcgac ggccgtggcg ccggcgatca ggaactccag cgcgtcgtca gccgtcatga
      601 tcccgcccat cccgatcagc gggagcttga ccgcccgcgc cacctcccat accattcgga
      661 ccgcaatagg ccggatggcc ggaccggaga gcccaccagt gacgttgcca agctttggac
      721 gacggcttcg cacgtcaatg gccataccga tcagcgtatt aatgagggag agcgcgtcgg
      781 ccccggcatc cgccagcgcc cgcgcaattt ccaccgcatc ggtaacattc ggggacagtt
      841 tgggaatgag ggggaggggc gtggcctgtc gcacgcaggc gaccagtttg ttggccagcg
      901 tggcatcgca gccgaagatc aggccgtcag ccacgttggg acaggagatg ttcaactcga
      961 atccgctaat gccttcgtga tcgctgagcc gtttcgccag ctcaacatag tcctcgatcg
     1021 actcaccggc gatattcaca atgacaggcg gacccaacgt cctgaggtag gggagtttct
     1081 cctcgatgaa cgcctgcacg ccgacattct gcaggccgat ggcattgagc atcccggccg
     1141 gggtctcgac gatacggggc ggcggattcc cggcccgcgg cgtacgtgtg atcgtcttga
     1201 cgacgatggc gcccaggcga gagaggtcca gaaacggttc gaactcctga gcatagccaa
     1261 aggtcccgga ggcggtcatc accgggttct gcatacggat gcccgcaaca gtgacgctta
     1321 aatccgtttt cacgccttgc ccttcatgct tcgcctacct tgcgtcagtt cctcttgagc
     1381 ggctgcttgg tagcactgct gaagacggcg cgtgactgga ccggggctgc ccgcaccgat
     1441 cgtgcagccg tcaatcgcga tcaagggcgt gatctccttg agggagccgg tgagaaacgc
     1501 ctcatcagtg gctaggagct catcgagcgg gaccggagcc tcatggacga tcaggccttc
     1561 tcgccttgcc agctcgatga ccacttccct ggtgattccc ggtaagagcc ccgagtcctc
     1621 tgggggagtc gtcagcatcc cttgtgagat cgaaaacaga ttgctcgtgg tcccctcgat
     1681 gacgcagcca tcagcgccca caaacaacgc ctccactgcg ccttcacgtt tcgcgtgcgc
     1741 catcgccagc atgttataga gatagtcaag cgacttgatc cggagtgggt tgaacggact
     1801 cccccagcga atcgtgaccg cgccgatccc gacctgctgg cgctctgcga ttcccgcatc
     1861 caaaggcttg gcgaccaata gtagcgtggg tgacgggggc gcgtctggag gcaggaggga
     1921 acccatcacg tccgggcctc tcgttaccgt cagccgcaaa gacgcatcgg cgtcctgaag
     1981 gcgattgcgc ctgagcaact cacccatcac cttgcgccag gtgcccacgt cgccttcaaa
     2041 cggaatcccg atctggtcgg cgcctttctt gaggcgcccc aggtgccgct caaggccaaa
     2101 gatccacccg gtatacgccc ggatggtttc gaagagacca tcgccatacg agaacccgcg
     2161 atcgaacgcc gataccttcg cccgctcggc ctgcacatag cgtccattca gatagactac
     2221 ggccattcgc ttcctttacc tcccaaaacc ggcgctgacg ccgagcgtcc gaaagaaggt
     2281 ttcggccttg agcagcgtct cctcgtactc ccgtgccggc gaggaatcgg ccacgatccc
     2341 tccacccgtc tggaaataga tccgtctgcc tgtcacgatg gctgtccgaa tggcaatgtt
     2401 cagttccatc ccccccgaaa acccgatgaa tccgatcgcc ccggtataga ggccacgagc
     2461 ggtcggctcc acctcgtcga tcacttccat cgcgcggatc ttcggggccc cggtgatcga
     2521 cccgccgggg aaggtcgctc gcagacagtc gatcgggtct gttccgtcct gaagggtgcc
     2581 cgccacggtc gacaccatgt ggtggagggt gtggtaggtc tcgatcgtct cgaactgctc
     2641 gacatggacg gagccgacct gacagattct gcccagatcg ttgcgctcca gatccacgat
     2701 catgacatgt tcggcgcatt ctttcggatc gtgacgaagg tgaccgatga tccgcgcatc
     2761 gtcctccacg gtcatcccgc gagggcgagt tcccttaatg gggcaggttg agatccgatt
     2821 cccctccacc agcagaaatc gttccgggga gttggacagt acatggaagg ggccacagtg
     2881 aaggtacgcc ccgaagggcg cgggaaagcg actccggagg cgttggtaca gcgcccatgg
     2941 ttcgccggag aactgtgtgg cgaagcgctg cgagatattg gcctgataga tatcgccggc
     3001 tgcgatatat tccaacaccg tctccagcgc cttacagtac tgcgctttcg acatgttgct
     3061 ctcgaggggg gtctcgagat gcgacaactc aggcggcccg ctcccgccgg tcagcccggc
     3121 gcgcgcgatc aggtcacgac cctcctgcaa ccgttccagg gctcttcgct cacgcgcgat
     3181 acccggcggc agcggcaaac cggaggaggt gacgagcagc tcgccatttg caggctcaaa
     3241 ccagaagaga cggtcgtaaa aatggatcac gcagtcaggc agcctcaggt cgtctaccgc
     3301 tacggccggc agtctttcga ggtggtgccg gaggtcatat ccgaagtagc cgatggcgcc
     3361 ggctatcggc acgtccccgg cagaggtcac gtcaccatac tgtctgagag ccgcctgaag
     3421 gtgttggaac ggatcacctc tgacccggcg cgactcgcct tggagtgaga tggtgatctc
     3481 atctcctttt gccgagaagg tgaggaacgg atcgtaaccg aagcatggag accgccagtg
     3541 attcacagcc catggatcag cgtccaaaag gctgaaaccg ggctcggcgg caaccagacc
     3601 caacagatcc gtgagggccg gcggattcga gacttcctca atgaggaggt gaggatcggt
     3661 gtgcgaggtc ccgcgcatgg aggtgggaaa cgacaaggtc atatccggtc cattttgtcc
     3721 atttggtcta tccggtttat ctggtctgtt gaccgaacaa acggaataga cgagttagac
     3781 cagaggactt cgtggccgtc gaagacgggc ccatccttac agacaagccg gtaggcccgc
     3841 cctgggtcgt ctcccttcac cggtacgacg cagcccatgc aggcgccgaa cccgcaagcc
     3901 atgttcgcct ccatggaggc ttggtatggc aggttgtatt ttttggcaat cggcgcaatg
     3961 gccgacagca ttgggtgcgg gccgcaggcg tagagacgag tgttgagtgc tgagtattga
     4021 gtgttgagaa actgctccag caggttcgtg accagtcctt tatggccgag actgccgtct
     4081 tctgtagtca ggtggaccgt ggcgccgatt cgccgaaagt cggaaacaca cagcaggtca
     4141 gccttggatc gtccgccgag aaaaacggcc atcttcgttc cgcgtgccgc gcgacgcgca
     4201 gccagcgctt cggcaagagc ggcgataggg ggtacgccga ttccaccggc aatcaggacg
     4261 gcgctcgtca cttttctggg aatctcaaag ccatttccga gcggaccgag caatcgcaaa
     4321 gatcttccag gacgtgtcgt ggccaggagc gtcgtacctt tgccgcacac cttgtagagg
     4381 atctccacct ccgccatccg ggtttcgggt ttggggtttc gggtttgggg ttctacacct
     4441 tgaaccttga accttgaacc ttgaacctct ctcacgcgtc tgatgctcat gggccgaggc
     4501 aacagcggat ctcgtccatc ggtccatccg agcatgagaa actgcccggg acggaatcgt
     4561 gtcaggcgtg ccggaccggc cagacgcatc gaaaagtatg acggcgcaat ttcccgattt
     4621 gccgtgactt caacctgaca ctcggttggg ctcatcaccg gactcattcc tgcggttcca
     4681 tgctccgggt tctatcctca aaactcgcaa ctcgaaactc aaaactattc tgcgtgatac
     4741 tcttgcagcg cctggaccct gagcttcccg tgtcgcatcg cctggattgc ttcgaccgcc
     4801 gcacacgccg cggccatcgt ggtaaagtag gggatgccca tcgtcacggc tgtccgccgg
     4861 atggagtagg agtcgagacg cgcatggtga ccctcaggag tattaatgac caggccgatc
     4921 t
//
LOCUS       DATMXI010000238         5850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_3588, whole genome shotgun sequence.
ACCESSION   DATMXI010000238 DATMXI010000000
VERSION     DATMXI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3588"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VN444_04500"
     CDS             <1..441
                     /locus_tag="VN444_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR21105.1"
                     /translation="AKFATKVTLIHRRDKLRASKIMQERAFKNPKIEFLWNRTIVEIL
                     DVSQGKVTGVTTRVDGSTSLETLACDGVFVAIGHSPNTALFSRQLEMDERGYIVTHHG
                     TMTSIPGVFAAGDVQDHVYKQAITAAGSGCMAALDAERYLENLG"
     gene            542..1738
                     /gene="glp"
                     /locus_tag="VN444_04505"
     CDS             542..1738
                     /gene="glp"
                     /locus_tag="VN444_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWR21106.1"
                     /translation="MKTLLGLEEGMRIVMDAAQPIERTEQVALLEAAGRVLSEEICAS
                     MDVPPFARAAMDGYAVRAEDTFGAGNFSPKILELIEVIHAGESARRSVRPGSCIQVAT
                     GAPMPEGADAVVMVEDTELDSAKVKVYKPVYPQQHASPMGEDIPQGSLVLQRGVRLDP
                     SKIGVLAALGLQEVGVYQKPKVAVIPSGNEILMPGEVLSPGKIYDINSYTLSALINEN
                     GGLPHIFPIMKDTLESTISTIREALAYDLIVLSGGSSVGERDMMLEAVQRMGELKFHG
                     IAVKPGKPTLCGIVEGHLLLGMPGYPTSCLTNGYGILAPALRKMARLSQRTLASLKAP
                     MSRRYTSTIGRHQYLPVRLDAGEVVPAFKESGAITSMADAEGYIEIPANVDLLEKGEV
                     VAVLLF"
     gene            2024..3226
                     /locus_tag="VN444_04510"
     CDS             2024..3226
                     /locus_tag="VN444_04510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWR21107.1"
                     /translation="MDLTLVKQLAISSSSKIVLLVADGLGGLPRETDGRSEMEVARLP
                     NLDALAARSLCGLIDMVGPGIIPGSGPGHLALFGYDPFTYQIGRGVLEACGIDLELRS
                     SDVASRGNFCTLDEEGRVIDRRAGRITTETCQRLCAKLDQIRIEGVEVIVRPVKEHRF
                     VVVFRGEGLSDALSESDPLVTGERPLPVKGIGPGAERTAELVNQFLDQAREALKEEKP
                     ANMVLLRGFAAPPQLPAFPDLLRLRAVAITCYPMYRGLAKLIGMEPLPFCADLDDELR
                     TLSRNYDQYDFFYVHFKGTDRAGEDGDFDAKVAALEELDRRIPHFLDLHPDVFIVTGD
                     HSTPSILKGHSWHPVPFLLWSRWSRSAGVSRFTERECAQGPLGRMPAVDLMPLAMANA
                     LRFKKFGA"
     gene            3237..4439
                     /locus_tag="VN444_04515"
     CDS             3237..4439
                     /locus_tag="VN444_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR21108.1"
                     /translation="MSKVGLYWRLLQGVVENPRYYYFILRRSAYDKTVRQLANNNLPE
                     KGTFYPVKLDLRVIYGCNLRCKMCGQWGDTGTYFSYDTAKLQRKLDLQVIEGVVKELV
                     PHGLRYVDLEGGETFLYPQIIELLRMLKRHRLFVKPVTNGTLLKKYAGELIDTGIDAI
                     HVSVDGDKEAHNFVRQADWAYDKMLEGLEAIIVERQRRNRHTPLVHVSFTMTRHNKSL
                     SVYKLCEELAGKGLLDVLCIKSNPIWVSAANGEAYNKMIAEYFDISGLTSWTGFVEDY
                     RDFEEEAKEIAQTIRQLKTKDYDFFVHHLPSIPLTEIPKLYTDYNWNLGRTHCPVPYL
                     EPTIDVDGHVYPCNLFTDEPLSMGNVNEQPFLDIWFGDKYQTFRRMLSEQGGLLPICN
                     RCCQLTEH"
     gene            4451..5497
                     /locus_tag="VN444_04520"
     CDS             4451..5497
                     /locus_tag="VN444_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWR21109.1"
                     /translation="MSQETNTRSSQIFHVLAPLAGVVLFSYLIYKAGARELWEGVVRF
                     GFGIVLFLALEGVGDVMRAIATRHCFSSDSPKVPLVSLLYMRCTGAAYNFITPTSGFG
                     GEVVKVMLLEKHVSRSEAARVVIIDKFTYGIVQFGMAFLGSAAVLFWTPLDLGLKLTF
                     WAASVAMWGGFIGLLVLQRQGWFSGFLGRAVGVVAGRKAREWIETNLAELDGRLRSHY
                     EKQGKDLILAMFWHALAFTMGIVQAGLFLWFVFGVDDWAKASAIWFFGSWFDCIIFMV
                     PAGLGTQELTRALIFERAIGFTFSEGVAFSMILRINQIFWTAVGLGTYALEMSLSRRR
                     LGGALVKIESDTGL"
BASE COUNT         1199 a         1560 c         1807 g         1284 t
ORIGIN      
        1 gcgaagttcg cgacaaaggt cacgctgatt catcggcgtg ataagcttcg ggcgtcgaag
       61 atcatgcaag aacgggcctt caagaatccg aagatcgagt tcttgtggaa tcgaacgatt
      121 gtagagatcc ttgacgtgtc acaaggaaag gtcaccgggg ttacgaccag ggtcgatggt
      181 tcgacctcgc tggagacgct cgcctgcgat ggggtgttcg ttgcgatcgg tcattcccct
      241 aataccgcgc tgttctcgag gcagttagag atggatgagc gagggtacat tgtgacgcat
      301 cacgggacga tgaccagcat ccccggtgtc tttgctgccg gcgatgtgca ggaccatgtc
      361 tacaagcagg ccatcaccgc cgccggatcg gggtgtatgg ccgcccttga tgccgagcgc
      421 tacctggaga acctcggata ggaaggtaag cgatcagccg tcagctatca gcttacagca
      481 agggtcccgg ttctgattgc tgattgctga aggctgaact aagatcatgc caatgaaacc
      541 gatgaagacc ctcctcggcc tggaggaggg gatgcgaatt gttatggatg cggcccagcc
      601 gatagagcgg actgagcagg tggcgctgct tgaggcggcc ggccgggttc tgtccgagga
      661 gatctgcgca tcgatggatg tcccgccctt tgctcgggcg gccatggacg ggtatgcggt
      721 cagggcagag gatacgttcg gcgctgggaa cttttcaccc aagattctgg agctcattga
      781 ggtgattcac gccggagagt ccgcccgccg gagcgtccgt ccggggagct gcatccaggt
      841 ggcgacgggc gccccgatgc ccgagggcgc cgatgccgtc gtgatggtgg aagatacgga
      901 gctggacagc gccaaggtga aggtctacaa gcccgtctat ccccaacagc acgcctcgcc
      961 gatgggcgag gacattccgc agggcagcct cgttctgcaa cgcggggtac gcctggaccc
     1021 gagcaagatc ggcgtgctgg cggcccttgg gctgcaagag gttggggtct atcagaagcc
     1081 caaggtggcc gtgatccctt ccggaaacga aatcctcatg cccggagagg ttcttagtcc
     1141 cgggaaaatc tacgacatca actcgtatac cctctcagca ctcatcaacg agaacggagg
     1201 ccttccgcac atcttcccga tcatgaagga cacgttggaa tccacgatct cgacgatccg
     1261 agaggccctc gcgtacgatc tcatcgtcct gtctggaggc agctctgtgg gagagcggga
     1321 tatgatgctg gaggcggtgc aacggatggg ggagctgaag ttccacggta tcgccgtcaa
     1381 gcctggaaag cccaccctgt gcggcatcgt ggagggccat ctgttgctcg ggatgccggg
     1441 ttatcccacg tcatgcctga ccaacggcta tgggatcctg gcccccgcgc ttcggaagat
     1501 ggcaaggctg agccagcgta ctctcgcctc gctgaaagcc ccgatgtccc gtcggtacac
     1561 ctcgacgatc gggcggcacc aatacttgcc cgtccgtctc gatgccgggg aggttgtccc
     1621 ggccttcaaa gagtcgggcg ccatcacctc gatggcagac gcagaggggt atatcgagat
     1681 ccctgccaat gtagacctcc tcgagaaggg ggaagtcgtg gcggtcctcc tgttttgacc
     1741 attcgtccgg ttatcctcgg cgccatttcc tggcagcgtt gtccggaggc gataccttca
     1801 tggatcgtaa ctacaaggta gataggctga aggcttttag gggtacatca tgcgtgatca
     1861 cacaaaactt ctggcatgtg aattggttga tgaagtagca atggtggttt atcgggttga
     1921 actaaagatc gtagaaaccg agaaggtctt gaatggcttg attcgtgctt tgcgagataa
     1981 ttcagagcct tcagccttca gcctaaacac ctgagtcgtt accatggatc taacacttgt
     2041 caagcagcta gccatttcct catcctccaa gatcgttctg ttggtagctg atgggttggg
     2101 gggcctaccg agagagacgg atggccgaag cgagatggag gtggccagac tccccaatct
     2161 cgacgccctc gcagcgcgat ctctctgcgg tctcatcgac atggtgggtc ccgggattat
     2221 ccctggcagt ggaccggggc atctcgccct ctttggttac gatcctttca cataccaaat
     2281 tggacgaggg gttcttgaag cgtgcggcat cgacctggaa cttcgttcaa gcgatgtggc
     2341 aagccggggc aacttctgta cactcgatga agagggaagg gttatcgatc ggcgcgccgg
     2401 tcgaattacg actgagacct gccagcgcct ctgtgccaag ctcgatcaga tccggatcga
     2461 gggggtggag gttattgtca ggcccgtcaa ggagcatcgg tttgtcgtcg tgtttcgggg
     2521 ggaggggctc tctgatgccc tttccgaatc cgatcccctg gtcacaggtg agcgacccct
     2581 tcccgtaaag ggtatcggcc cgggagcgga acggacggcg gagcttgtca atcagttcct
     2641 ggatcaggcg cgtgaggcac tgaaggagga aaagccggca aatatggtgc tactcagggg
     2701 atttgccgcg ccgccacagc tccctgcatt tccggatctg ttgcggctcc gggcggtagc
     2761 gattacctgt tatccgatgt accgcggtct cgccaagctc atcgggatgg agccgttacc
     2821 cttttgtgcc gacctggatg acgagctgcg cacattgtcc cggaactacg accagtatga
     2881 ctttttctac gtgcatttca aagggaccga tcgggccgga gaggacggcg actttgacgc
     2941 gaaagttgcc gcactggaag agctggatcg gcgaattccg cacttccttg atttacaccc
     3001 cgatgtgttt atcgtgaccg gcgaccattc gacgccgtct atcctgaaag gccatagctg
     3061 gcatccggtg cccttcctgc tctggtcacg atggtctcgt tccgccgggg tcagcagatt
     3121 tacggaaaga gagtgcgctc agggaccttt agggaggatg ccggcagtcg atcttatgcc
     3181 cctggcaatg gccaatgctc tgaggttcaa gaagtttggg gcatgagggg taatgtatgt
     3241 caaaagtcgg cctctactgg cgcctccttc agggcgttgt cgaaaatccg cgctactact
     3301 acttcatcct ccgaaggagc gcctacgaca agactgtcag acaactcgcg aacaataacc
     3361 ttcctgaaaa ggggaccttt tatcccgtca agctcgacct gcgcgtcatc tacggctgta
     3421 acctccgctg taagatgtgc ggccagtggg gtgacacggg aacgtatttc agttacgata
     3481 ccgccaagct ccagcgtaaa ctcgatcttc aggtgatcga gggggttgtg aaggagcttg
     3541 tgccgcacgg cctcagatac gtcgatttgg aggggggcga gacgtttctc tatccgcaga
     3601 tcatcgagct cctccgaatg ctcaagcgcc atcgtctctt cgtgaagcct gtcaccaacg
     3661 gcacgctctt aaaaaagtat gcgggcgagt taatcgacac ggggatcgat gcgatccatg
     3721 tgtcggtcga tggcgacaag gaggcgcaca acttcgtacg tcaagcagat tgggcgtacg
     3781 ataagatgct ggaggggttg gaggcgatta ttgtggagcg gcagcgccgc aaccgtcata
     3841 cgccgcttgt gcatgtgagc ttcaccatga cgcgccacaa caagtccctg tcggtctata
     3901 agctctgcga ggagctggcc gggaaggggc tgctggacgt cctgtgcatc aagtcgaacc
     3961 cgatttgggt gtctgcggcc aatggcgagg cctataataa gatgatcgca gagtacttcg
     4021 atatcagcgg cctcacgagc tggacggggt tcgtcgagga ctatcgcgat ttcgaagagg
     4081 aggcgaagga gatcgcgcag actatccgcc agctcaagac caaggattat gactttttcg
     4141 tccaccacct tccctcgatt ccattgacgg agataccgaa actctacacg gactacaact
     4201 ggaacctcgg tcgcacgcac tgcccagtgc cctaccttga gccgacgatc gatgtcgacg
     4261 gtcacgtcta cccgtgcaac ctctttacag atgagccgct ttcgatgggg aacgtcaacg
     4321 agcagccgtt tctcgatatc tggttcggcg acaagtatca gaccttccgg cggatgctgt
     4381 cggagcaggg cggcctgctg ccaatctgca accgctgctg tcagcttacc gaacattaga
     4441 tgaaccggca atgagccagg aaacgaatac gcgcagttcc caaatcttcc acgttcttgc
     4501 gccgcttgcc ggcgttgtgc tcttcagcta cctgatctat aaagcgggag cgcgtgagct
     4561 gtgggaagga gtcgtacgct tcgggtttgg gattgtcctc ttcctggcgc tggagggggt
     4621 gggcgacgtc atgcgcgcca tagcgacacg ccactgcttc agctcggaca gcccgaaggt
     4681 tccgctggtg agcctgctgt acatgcgctg taccggagcg gcatacaact tcatcactcc
     4741 gacctctggg tttggtggtg aagtggtcaa ggtgatgctc ctggaaaagc atgtctcgcg
     4801 tagcgaggcg gcgagagtgg tgatcatcga caagttcacg tacggcatcg tacagttcgg
     4861 gatggccttt ctggggtcgg cggcggtcct attctggacg ccgctagacc tgggcctgaa
     4921 gctcacattc tgggccgcga gcgtggccat gtggggcggc ttcatcggtc ttctggtctt
     4981 gcagcggcag gggtggttca gcggatttct cggccgtgcc gtaggggttg ttgccgggcg
     5041 gaaggcgcgc gagtggatcg agacgaatct tgccgagttg gacggccggc tgcgcagtca
     5101 ctacgaaaag caggggaagg atttgattct ggcgatgttc tggcacgccc tggcgtttac
     5161 gatggggatc gtgcaggcgg ggctcttcct ctggtttgtc ttcggcgtgg atgactgggc
     5221 caaggcgtcc gcgatctggt tcttcggcag ttggttcgac tgcattattt tcatggttcc
     5281 ggccgggctc gggacgcagg agctgactcg cgcgctgatc ttcgaaaggg cgatcggctt
     5341 taccttcagc gagggtgtgg cgttttcgat gatcctgcga atcaaccaga tattctggac
     5401 tgcagtcggc ctggggacgt acgcgctgga gatgtcgctg agccggcgac ggctgggagg
     5461 cgcgcttgtc aagatagaat cagacaccgg actgtaaggt gaactccgtc gtgaatcggc
     5521 tttgtcggat aaactatgat gagatgttgg acgcgtatcc ttatcctgta ctcctacttt
     5581 caaatccccc tcaatccccc tttctcaaag ggggaggtag agtgaatacc cctctttgga
     5641 aaagaggggc gagagcctgc ccctgcatgt attaagcagg ggggagattt caacaatgct
     5701 gtccacatcc ggtctttcac aaatcctata ttggacagga tggtggagca taatgcttct
     5761 gcggagactg ccactccagc attgaaattg acaatcgtgt aacagtatct ttagaatata
     5821 actgttgagc aatcagcgat cagtaagagc
//
LOCUS       DATMXI010000239         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_233261, whole genome shotgun sequence.
ACCESSION   DATMXI010000239 DATMXI010000000
VERSION     DATMXI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233261"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VN444_04525"
     CDS             <1..945
                     /locus_tag="VN444_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWR21110.1"
                     /translation="TVTSEGFAQTFIRTKTSANSQWTTVAVDQTAGSNLSTRIDGIIT
                     GQTFDYRASRVNILNPSLYRDTDITSKTAPSDASAPGAPGTPVVLDKHLKSITLTWTA
                     ASGGDIDDYEWEVRTAASGGGSQVATGRAGTIKTSLTLNTIAYSATRYFRVRAKDYSG
                     NVGAWSADRSFSFSKVLTIDVDDDQVTTPKLPNNAITASGAYSNDGTATANTGSQVVE
                     IGTVTVTTDGGAVIVVAKAMTQIDAGAYGFVRLRKTSTSGTVLDEVPLYTTYDSVGLI
                     GYDASPSASQTYKLVLEWVSGANIYAFYRRLVTFNLKK"
     gene            955..1233
                     /locus_tag="VN444_04530"
     CDS             955..1233
                     /locus_tag="VN444_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21111.1"
                     /translation="MKAALLIDKLTNTLDGTIIVSVDSDIDFVQPTDAHDVLTIPTDH
                     PAVYEQLEWEAPKKILLGAEWGRELKRKVREPIVPPSEARRSDREPTT"
     gene            1230..1463
                     /locus_tag="VN444_04535"
     CDS             1230..1463
                     /locus_tag="VN444_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21112.1"
                     /translation="MTDLKPIIDLIQAALIPGLLYGIKLLWDIRDHLARLNGRVTKCE
                     ELRQAHERSDKEKHDSCEERIAFIEHTMIGRKG"
     gene            1562..2392
                     /locus_tag="VN444_04540"
     CDS             1562..2392
                     /locus_tag="VN444_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21113.1"
                     /translation="MKTISLLLALLLSTSPALACDPWSIDRRESYGMNLSASDLLDPA
                     TTACRLTTYLREIEEEGFAKQVRIIFTPDWGPQILRDWADILRSKGFRVVAILSQGHS
                     DSELAAQQAWAQYGLPQIADILDAVQPQNESNDTTGWSPEQYAQWHRALVPVIRAAVP
                     GVPIMSPTLNNGANWVSWHHGTGLIPGQDYDILAANVTNWSKKTLKKVAKIAVAEGLP
                     VWLTETRWDEAAKLHAMGVNVQRSYVFVWNEWEGGSMAVRPGGTTPPTCPTYREGYPQ
                     "
     gene            2389..2829
                     /locus_tag="VN444_04545"
     CDS             2389..2829
                     /locus_tag="VN444_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HWR21114.1"
                     /translation="MITSAQCLTRFGDPTDERQRLAFESKWMTLWVPPPGIPALPRRI
                     YCHKWLMPRLIDFYERVEDAGIKDQIRTWDGCFNVRQKRGATSLSMHSWGLAFDINAA
                     WNRFGEPSTMSHELVACIELAGLDWGGRWRKPDSMHGQLAVWPS"
     gene            2953..3126
                     /locus_tag="VN444_04550"
     CDS             2953..3126
                     /locus_tag="VN444_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21115.1"
                     /translation="MIVVTVERQGGTMPDRALDGQTILHLLGVGGITTICLFVGYGLV
                     WVAIHIVEWWERG"
     gene            3133..3321
                     /locus_tag="VN444_04555"
     CDS             3133..3321
                     /locus_tag="VN444_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21116.1"
                     /translation="MDTKSLLTSKTFWGTVVQIAAMAAMFLKIDIGDQGGWVEAIVGV
                     VGAAVTIYGRIVAIKKIG"
     gene            3788..4309
                     /locus_tag="VN444_04560"
     CDS             3788..4309
                     /locus_tag="VN444_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21117.1"
                     /translation="MIPAALMIEAIPLVMGLARLIRGVLPDEETKLLVRWANLLKDGP
                     KNEAENEKAVEWKHDIRQFLSNLTLRRGFSPAYQYDQEIAIPEDLLTDIVDLLVGGWA
                     SSKWSLKTLTSNPENSRQGWAETVASGKPDLILAELTEWVARRGRTRSYSAVPAIRVD
                     ADWACDVGMFLIG"
BASE COUNT          982 a         1121 c         1345 g          884 t
ORIGIN      
        1 accgtgacga gcgagggctt cgcgcagacg ttcatccgca ccaaaacgtc cgcgaattcg
       61 caatggacga cggtcgctgt agaccaaacc gcaggatcga acctctccac tcggattgat
      121 gggattatca ccgggcagac atttgactac cgagcgagcc gtgtcaatat cctgaacccc
      181 tcgctctatc gggataccga tattaccagc aagaccgcgc cgagcgatgc cagcgcaccg
      241 ggagcaccgg ggacgccggt cgtgctcgac aagcacctca agagcatcac cttgacatgg
      301 acagcggcga gcggtgggga catcgatgac tacgagtggg aagtgcgtac agcagcgagc
      361 ggaggtggct cgcaagtcgc aacaggacgt gcaggaacga tcaagaccag cctcaccctg
      421 aatacgattg cctactctgc cactcgctat ttccgggtac gagccaaaga ctacagcggc
      481 aatgtcgggg cgtggagtgc cgaccggagc ttcagtttct cgaaagtcct cacgattgat
      541 gtcgatgacg atcaagtgac gacgccgaag ctcccgaata atgcgattac ggcgagcggg
      601 gcctatagca atgatggaac ggcaacggca aacactggat cacaggtcgt cgaaatcggg
      661 acagtcaccg tcacgacgga tgggggggcc gtcatagtcg tcgcgaaagc catgacgcaa
      721 attgatgcag gggcctatgg gtttgtacgg ctccgcaaga ctagcacctc cgggactgtg
      781 ctagatgagg tgccgctgta caccacatac gattctgtgg gcctcattgg atatgatgcg
      841 agtcccagcg catcacaaac ctacaaatta gttttagagt gggtctcagg ggccaacatc
      901 tatgcgtttt atcggcggct tgttacattc aatctgaaaa aataggcggg ggccatgaag
      961 gctgcattgt tgatcgataa gcttacaaac acgcttgacg gcactattat cgtctcagtc
     1021 gatagtgata ttgacttcgt gcagcccaca gatgcgcatg acgtattgac tatacccacc
     1081 gatcatccag cggtctacga gcagttagag tgggaagccc caaagaaaat cctcttaggt
     1141 gctgaatggg gtcgagaatt gaaaagaaaa gtacgagagc cgattgttcc cccatcagag
     1201 gcgcgacgct cagacaggga gccgacaaca tgacagacct gaagcctatc attgacctga
     1261 tccaagcggc actgatcccc ggcctgttat acggaatcaa gctcctgtgg gacatccgcg
     1321 atcacctggc acgactgaat ggacgagtga caaaatgtga ggaactccgc caggcgcacg
     1381 agcggagtga taaggaaaag catgattcgt gcgaagagcg tatcgcgttt atcgagcaca
     1441 ccatgatcgg gcgaaaggga taaggtggag cgctggcttg gcgtcaacta tccctgtaat
     1501 gctgcctgtt gtctcgcgct tcgggggtcg gtgtatccgc taccctgcaa aaaggagtcc
     1561 aatgaaaacg atcagcctac tacttgccct gctgctctcc acctcgcccg cccttgcatg
     1621 tgatccctgg tcaattgata gacgtgagag ctatggcatg aatttatcag ccagtgactt
     1681 attagatccg gccacgacgg cgtgtcggtt gacgacctat cttcgggaga ttgaggagga
     1741 aggattcgcc aagcaggtca ggatcatctt tacgcctgat tggggaccgc agattctcag
     1801 ggattgggct gacatcctcc gtagtaaggg gtttcgggtc gtggcgatcc tgagccaggg
     1861 gcacagcgat tcagaactcg cggcgcaaca ggcatgggcg cagtacggcc tgccgcagat
     1921 tgccgacatc ctggatgcgg tccagccgca gaatgagtcg aacgatacga cggggtggag
     1981 tccggagcag tatgcccagt ggcatcgggc gctcgtcccg gtgattcggg cggccgtccc
     2041 cggcgtgccg atcatgtcac cgaccctgaa caacggcgcg aattgggtct catggcacca
     2101 cggcacaggg ctgattcctg gtcaggacta tgacattctc gcggccaatg tgacgaattg
     2161 gtccaagaag acgttgaaga aagtggcaaa gatcgctgtg gccgaggggt tgccggtgtg
     2221 gctgactgag acacgatggg atgaagccgc gaaactccat gcgatgggcg tcaacgtgca
     2281 acggtcatat gtgtttgtgt ggaatgagtg ggaaggtggc tcaatggctg tccggccagg
     2341 ggggacaacg ccgccgacct gtccgacgta ccgagagggg tatccacaat gattacatct
     2401 gcacaatgtc tcacccgctt tggcgacccg accgacgaac ggcaacggct cgcctttgag
     2461 tcgaaatgga tgacgctttg ggtgcccccg ccgggtattc cggcgctgcc acggcgcatc
     2521 tattgtcaca agtggctgat gcctcgcttg atcgacttct atgagcgggt cgaggatgcg
     2581 ggaatcaagg atcagatccg gacgtgggac ggctgtttca acgtccgcca gaagcgtggg
     2641 gccactagcc tgtccatgca ctcatggggg ttggcattcg acattaatgc cgcctggaat
     2701 cgattcggcg agcccagcac gatgagtcat gagttggtcg cgtgcatcga gctggcgggc
     2761 cttgactggg gtgggcgatg gagaaagcca gatagcatgc acggccagct tgctgtgtgg
     2821 ccaagctgat gtacggcgcg tgtcaggccg taacctgtta tggcccctgt ccagggcgag
     2881 ccgtctcgcc gaccgtgaag gtctgctact ggcacctgga acagcgacac ggtgagcagg
     2941 agcgatgtca gggtgatcgt tgtgactgtc gagagacagg gggggaccat gccggacagg
     3001 gcgcttgacg gccagacgat tctgcacctg ctcggtgtag gcggcattac gacgatctgc
     3061 ttattcgtgg gctacggtct ggtgtgggtc gcgatacaca ttgtcgaatg gtgggagagg
     3121 gggtgagcag agatggatac aaagagcctg ctaacgtcaa aaacattctg gggcaccgtc
     3181 gtacaaattg ctgcgatggc cgcgatgttc ctcaagatcg acatcggcga tcaaggcgga
     3241 tgggtcgagg cgattgttgg ggtggtcggt gctgccgtaa caatctacgg gcgaattgtc
     3301 gcaatcaaaa agatcggcta acacctgcac tgcctcaatg gtccgaaccc ggcgaggcag
     3361 cactataagg gggcgtgaca tgcggcgatg ggtgattgtg ttccttgtca tcattggact
     3421 atccgggtgt accgtcgatc caaggtgcgt gctatttttt gacctatcga agtgcccagg
     3481 gcagcgataa catgcacaca acagctctat cgtggtcgat gcaagtctcg tgcaattggt
     3541 tgtattggtg tcagcagtgt aagatacccc taccccaggg ctatgcctgt ccgcgttgcc
     3601 gcaccgactg gtatatgtgg gggccgtgtc gatcctcaac tgatacagca cggtggaaac
     3661 gagtcgttgc gatggtgtgc atcgtcattc tgctgagcgc ctgcggtgga cgttacagcg
     3721 ttccagacca gcgtgatatt caaaatccgg agatcggggg ccgagtgttt ggtccggttg
     3781 tgccgaaatg atcccggcag ctctgatgat cgaggcgatc cccttggtga tgggccttgc
     3841 acggctcatt cgaggtgtcc tgcctgatga agagacgaag ctgctggtac gctgggctaa
     3901 cctgctcaaa gatgggccaa agaatgaggc agagaacgag aaggccgtcg agtggaaaca
     3961 cgacatccgg caattcctgt cgaatcttac actacgccgt ggcttctcgc cagcctacca
     4021 atatgaccag gagattgcca tcccggagga tctactgacc gacatcgtag acctgctggt
     4081 tggaggatgg gcgtcatcga agtggagtct caaaaccttg acgagcaatc ctgagaatag
     4141 tcggcaagga tgggctgaga cagtcgcatc ggggaaacct gatctgattc tcgcggaatt
     4201 gaccgaatgg gtcgccaggc ggggccgtac ccggtcatac agcgcggtcc cggcgattcg
     4261 agtcgatgcg gattgggcgt gtgatgtggg catgttcctg attggataaa agacagcccc
     4321 cgctcacgct tc
//
LOCUS       DATMXI010000240         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_197587, whole genome shotgun sequence.
ACCESSION   DATMXI010000240 DATMXI010000000
VERSION     DATMXI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197587"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VN444_04565"
     CDS             <1..122
                     /locus_tag="VN444_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWR21118.1"
                     /translation="KEWGLTDCTSFIVMEDQRLREAFTADHHFQQAGYTCLLR"
     gene            complement(308..478)
                     /locus_tag="VN444_04570"
     CDS             complement(308..478)
                     /locus_tag="VN444_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21119.1"
                     /translation="MYGQILGRRCDQPLLRKLTLCFSLLKCLSGLFGLFGQLGSEVPG
                     RQAIFMIIGTSA"
     gene            566..862
                     /locus_tag="VN444_04575"
     CDS             566..862
                     /locus_tag="VN444_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21120.1"
                     /translation="MDRSTHVHELLERATAILPASDEDLIYKGIAAGVSERIIALKKA
                     QARLQQKYHTVEELERKMTIEGVSPDDHTLYADYLEWRAIKHELTELVHIFETL"
     gene            859..1236
                     /locus_tag="VN444_04580"
     CDS             859..1236
                     /locus_tag="VN444_04580"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HWR21121.1"
                     /translation="MRVWYKAVIETLQASGLFSDVSLVGAKVRASLDDTRFLDIHLDP
                     TAQSYSYALIDLRLPYPGDKRVFGWDDYPHQGVVEIQHLERYPHHFQKRAEDGSWIFE
                     PSPMRGNIELDKRLEFCHISHVK"
     gene            1261..1566
                     /locus_tag="VN444_04585"
     CDS             1261..1566
                     /locus_tag="VN444_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR21122.1"
                     /translation="MTIVKASSKGQIVLPKEIRQKLDIRPGQKVVLRLVGDHAEIKPL
                     PRDPIESLRGIFKDYPGSLADELLTERRKDREREETEIARFARRPGLPQRRKGARKG"
     gene            1490..1891
                     /locus_tag="VN444_04590"
     CDS             1490..1891
                     /locus_tag="VN444_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWR21123.1"
                     /translation="MKKLKLLDSHAALVYLKGEKGHEKVKAALADAERSGVPLLMSEI
                     NIGEVGYILLRAKLTNNLDDFLSQFLSLPIRPVPVDFDLVIEAAKIKARYPLSYADAF
                     AVATALRNDAAIITGDPEFKAVMKLVQIDWL"
     gene            complement(1978..2136)
                     /locus_tag="VN444_04595"
     CDS             complement(1978..2136)
                     /locus_tag="VN444_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21124.1"
                     /translation="MTPQWLDSGTTPIDLRNAVMAKIDRQTPIGWSGPQGHGVAVQCG
                     TEAKDPPT"
BASE COUNT          509 a          665 c          538 g          478 t
ORIGIN      
        1 ataaggaatg gggactcaca gactgcacaa gctttatcgt catggaagac cagcgcctcc
       61 gcgaggcctt taccgccgac catcattttc agcaggccgg ctacacctgc ctccttcgct
      121 gatcttctcg tccgcctccc agcctctagc tttcccgcct ccccgcctta caggtcacca
      181 ttcgtagcga tcctttgggc caattccctg gccaaccgat aatgcatcgg tttctcgatg
      241 tagtggccga cctcctcgtc tcaaatcccc ccaacctccc tttacaaagg ggggcgaggg
      301 gggatttcta tgccgacgtc ccgataatca tgaaaatcgc ctggcgtcca ggcacctcgg
      361 aacccaactg accaaagaga ccaaagagac cagacagaca tttcagcagg ctgaagcaga
      421 gggttaactt gcgcaacagt ggctgatcac accgccggcc caatatctgg ccatacatgg
      481 ctctccttgt tttgctcgaa gggcagatcg gtggtatagt aaaaaagcca aagcggacaa
      541 acccccaggc ggaaggagcc acgatatgga cagatcgact cacgttcacg aactgctgga
      601 aagagccaca gccatcctgc cggccagcga tgaagatctc atctataagg gaatcgccgc
      661 cggcgtgtca gagcgcatca tagccctcaa aaaagcgcag gcccggttgc agcagaaata
      721 tcacacggtg gaagagctgg agcgcaaaat gacgattgag ggtgtctcgc cagatgatca
      781 cacgctatac gctgattacc tcgagtggcg agcgattaag catgaactga ctgagcttgt
      841 gcacatcttc gagaccttat gagggtatgg tataaggcgg tcatagaaac gctgcaagcc
      901 agtggtcttt ttagcgatgt cagcctcgta ggtgccaagg ttcgcgcctc ccttgatgac
      961 actcgtttcc tggacatcca ccttgatccg actgcccaga gctactccta tgcactcatt
     1021 gatcttcggc ttccgtaccc tggcgataag cgagtgttcg gctgggatga ttatcctcac
     1081 caaggggtgg ttgagatcca gcacctggaa cgctacccgc accacttcca gaaacgagcg
     1141 gaagatggaa gctggatatt cgagccttct cccatgcgtg gcaatatcga gcttgacaaa
     1201 cggcttgagt tctgtcatat ttcacacgta aagtaaaacc tttttaccgg ggtaaaacac
     1261 atgaccattg taaaagcatc ctccaaagga cagattgttc ttcccaagga aatccgccag
     1321 aaactggaca ttcgtcccgg ccagaaggtc gtcttgcgcc ttgtgggaga tcacgctgag
     1381 atcaaaccgc tgccccggga cccgatcgag tcgctccgcg gcattttcaa agactatccc
     1441 ggatctctcg ccgatgagct tttaacagaa cgccgaaagg atcgagaacg tgaagaaact
     1501 gaaattgctc gattcgcacg ccgccctggt ctacctcaaa ggcgaaaagg ggcacgaaaa
     1561 ggttaaggcc gctctcgcgg acgccgaaag gtccggagtc cctctcctga tgtccgaaat
     1621 caacatcggt gaagttggct atatcctcct ccgcgcaaag ctgaccaaca acctcgatga
     1681 tttcttgagc caattcttat cccttccgat tcgcccggtg ccggttgact tcgatctcgt
     1741 catcgaggcc gcaaaaataa aagcgcgtta tccgctctcc tatgcagacg ccttcgccgt
     1801 cgccaccgcc ctccgaaacg acgcggcgat catcaccggc gacccagagt tcaaggcggt
     1861 gatgaaactg gtacaaatcg actggctgta gctccttcat ctactcttgc gctccttgtg
     1921 catgattctc cctcaagcct ttctttctgt cgccccgatc cccacttagg gccacgatca
     1981 ggtcggcgga tctttcgcct cagtaccgca ttgcacggct accccgtggc cttgcggccc
     2041 actccaaccg atgggcgtct ggcggtctat ttttgccatc accgcgttgc ggagatcgat
     2101 aggcgttgtc ccgctgtcta accactgtgg tgtcacctat gtctccgaac acctgttacc
     2161 ctgaccccgg tctgtacacc tcctccgcac
//
LOCUS       DATMXI010000241         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_271781, whole genome shotgun sequence.
ACCESSION   DATMXI010000241 DATMXI010000000
VERSION     DATMXI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271781"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VN444_04600"
     CDS             complement(<1..441)
                     /locus_tag="VN444_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR21125.1"
                     /translation="MRKRHNAAFKAKVALEAIKEERTLAQLSSDYGVHANQIGQWRKQ
                     LLEQLPTVFSERRKAGEQAREEQEAELYRQIGQLKVRGGGVEKQVTPASVSERRQMIE
                     PGHDRIPVGRQCELLGLPRASFYYRPRPITALEERLLRLIDEVYT"
     gene            complement(749..1486)
                     /locus_tag="VN444_04605"
     CDS             complement(749..1486)
                     /locus_tag="VN444_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWR21126.1"
                     /translation="MLSGGAAHAKSASDKDCSQVPSHSDLKAALTTARTAANGGFNLD
                     MWGTIVNRDGFVCAVAFTGADRGSQWPGSRVISAQKANTANAFSLTALSLSTANLWAA
                     VQPGGSLFGLQHSNPVDTGEAYDGSSSKFGTSTDPLVGGRMGGVNVFGGGLALYNSSH
                     VLVGALGVSGDTSCADHNIAWRTRHALNMDYVPSGVGADATRADNINYQGLVTVPSVA
                     NDFSHPICKIGGVDGVSAISLALPVTQ"
     gene            1949..2524
                     /locus_tag="VN444_04610"
     CDS             1949..2524
                     /locus_tag="VN444_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWR21127.1"
                     /translation="MERLTQKDVVRADAAEMTLLSQALELSGQGVILLTKNGRVLLIS
                     PRARAWLAGYFGASPSWSTNRLPDSLRRWRKRQEAQLGGADGVPPPRTPFISERGGKR
                     LVVRHLCNAETCHLFLEEQPTARKAAAFAPLGLTRREIEVLQWVAQGKTNMEIGSILG
                     LSHRTVQKHLEHVFKKLGVETRIAAATLVLG"
BASE COUNT          551 a          833 c          786 g          583 t
ORIGIN      
        1 ggtgtacacc tcgtcgatca gccgcaggag gcgttcctcc agcgcggtga tgggtcgcgg
       61 ccgatagtag aacgaggcgc gcggcagtcc cagcaactcg cactggcgcc ccacagggat
      121 acggtcgtgt ccgggctcga tcatctgtcg tcgctcactc accgaagctg gtgtgacttg
      181 tttttcaacc cctccacctc gaaccttcag ctgcccaatc tgccggtaca gctcggcctc
      241 ctgctcctcg cgggcttgtt ccccggcctt ccgcctctcc gagaagaccg tgggcagctg
      301 ctcgagcaac tgcttccgcc actgcccgat ctggttggca tggaccccgt agtcgctcga
      361 cagctgggcc agcgtgcgct cttccttgat cgcctcgagg gcgaccttgg ccttgaaggc
      421 tgcgttgtga cgcttcctca ttcccacgct cattacgctt ccttgttcac agcccgctca
      481 gcctctttgt ccaccttatt cccctgtcta gtgtttgggg agcattatag accccgcggc
      541 aagccgcggg gtatcgaccc tcaacgaaaa tgaacgccag gagggcggag cctcctggcg
      601 ttctagtgta gtgatgggtt tcagcgatca ttcgaacatc aaatcacgat acgcgctcgt
      661 caagccttct cttgacccgt cctgccatgc cagcgtcgcg caagacaact taggcgtcac
      721 ccaatgactc gtcttgccgt cacccaagtt attgcgttac cggcaacgca aggctgatcg
      781 ctgaaacacc gtcgacgcca ccaatcttac agatcggatg ggagaagtca ttcgccacgc
      841 ttggcaccgt cacaagccct tgataattga tgttatcggc gcgagttgcg tcggcaccaa
      901 ctccacttgg cacatagtcc atgttcaagg cgtgccgggt tcgccaggcg atgttatggt
      961 cagcgcacga cgtgtcaccg ctgacgccaa gagcgcctac tagtacgtgg gaagagttgt
     1021 acagggccaa accgccgccg aacacattca cgcctcccat cctgccacca accaggggat
     1081 cggtcgacgt accgaatttt gacgacgaac catcgtaggc ttccccggta tcgaccgggt
     1141 tgctgtgctg taagccaaac agcgaaccac cgggctgcac agccgcccac aagtttgcag
     1201 tggacagcga gagggccgtc aagctgaacg cattggctgt gttcgccttc tgggcggaga
     1261 tcacccggct gcccggccat tgagagccgc gatcggcgcc agtgaaggcg actgcgcaga
     1321 caaagccatc gcggttgacg atagtgcccc acatgtcgag gttaaatccc ccgttggctg
     1381 cggtacgagc ggtcgtgagg gccgccttga ggtccgagtg gctgggcact tgactgcagt
     1441 ctttgtcgga agcgcttttt gcgtgggcgg cgcctccgga cagcatgatt gtactgagga
     1501 gcatgccgct gatggcgaac gtgacggaca tcttcgtctt gaatcttttg ctgatcatca
     1561 taatgttctc cccatattca ttggccagcc ttagaatctc agactaccct ctgtgtgagt
     1621 gctccacgtt gtgactcaat gttatcccaa cactgcttgc aactcatccg tctgacatct
     1681 catggcggat ttcacgcctc gtggccccag aagcgtcttt atcgttcagc gctctacagc
     1741 ctcacctcct ttccagcctg ccacccgcac attgccgctc aaaactaaag ggatgcagac
     1801 gccgttgagg acaaaactcg ctggtctaca gtatacagat cgtcaagacc ggaccccttt
     1861 gattttcgtt gttatatcat gggcagccgt gtagtatcaa tacgcatttt ggcgtatata
     1921 gctgtgcctc cagcaggtgg agcatgggat ggagcggttg acgcagaagg atgtggtacg
     1981 cgcagatgcg gcggaaatga cacttctgag tcaggctctg gagctgagtg gacagggggt
     2041 gatcttgctc accaaaaacg ggcgggtgct gctgatctcg ccccgcgcgc gagcgtggct
     2101 cgcggggtac ttcggggcga gtccctcttg gtcaacaaat cgcctgcctg actccctccg
     2161 gcgttggaga aagcgtcagg aggcacaatt gggcggagcc gacggtgtcc cgcccccccg
     2221 cacaccgttc atatccgaac gaggaggaaa gcgcctcgtg gtgcggcacc tctgcaacgc
     2281 ggagacgtgc cacctgttcc tggaggagca gccaacggcg cggaaggccg cggctttcgc
     2341 acctctgggc ctgacccgtc gtgagatcga agtgctccaa tgggtggccc agggcaagac
     2401 caatatggag atcgggagca tcctcggact gagccatcgg actgtgcaga agcacctgga
     2461 gcacgtcttc aagaaactcg gggtggaaac gcgcatcgcg gccgctacct tggtgctggg
     2521 ttgacactcc agggcgtaaa ctgccccagg gaggagtccg gtcaaagaaa acaggagact
     2581 cgagcgcatg cagggccaga tcctcattgt gcaaacggcc ttttcaaact gcttgacagc
     2641 cgatctcatc tcttctaata taaatcccaa acgcatgact gcccttgctg gagagtccgg
     2701 tgagggcatt gctgcattaa gcgcaaatcg ggagggatct atggcttcag gta
//
LOCUS       DATMXI010000242         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_175939, whole genome shotgun sequence.
ACCESSION   DATMXI010000242 DATMXI010000000
VERSION     DATMXI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175939"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VN444_04615"
     CDS             complement(<1..538)
                     /locus_tag="VN444_04615"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWR21128.1"
                     /translation="MANRTYTVAVAGATGAVGETMLRLLEERNFPVRRLRLLASERSA
                     GKSLTFKGEEIKVENLGDGSFQGIEIALFSAGATRSLEFAPAAVKAGAVVIDNSSAFR
                     MQSDIPLVIPEINPDAIAGYQARGIIANPNCTTIVMLMPLKPLHDYGRVRRVIASSYQ
                     AVSGAGAKGIKELRRQTLA"
     gene            complement(554..2101)
                     /locus_tag="VN444_04620"
     CDS             complement(554..2101)
                     /locus_tag="VN444_04620"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWR21129.1"
                     /translation="MGNRILIFDTTLRDGEQAPGCSMNTREKLEMARQLARLKVDVIE
                     AGFAIASEDDFEAVKTIAQNLKGGPIIASLCRTRDIDIDRAWEALKYADRPRIHIFIA
                     TSEIHITYKLKSTQEEVMQAAIAAVKHARRYTDDVEFSPEDAHRSEQDYLCKVVEEVI
                     KAGATTINIPDTVGYGLPWEFGARIKNLFDRVPNIDKAVISCHCHNDLGLAVANSLEA
                     IRNGARQIECTINGIGERAGNASLEEIVMALRTRKDLLDFDTGINTEEIYRSSKLLTS
                     IIGIPVQPNKAIVGANAFAHEAGVHQHGMLQKPLTYEIMTPESVGVPQSRLVLGKHSG
                     RHAFKKRLEELGVMLPEDALNRAFTRFKVVADKKKEVFDDDLLAIVEEDALAAGETYT
                     LEYLQFTSGINIVPTATVRLKRENEIFQESGWGDGPVDAAYKAIDQITKVQGRLADYS
                     IRAITAGKDALGEVVLKLEVNGHTVIGRATSTDVIEASVRAYLNAINKIVGAARPPKD
                     MAAPRGL"
BASE COUNT          431 a          724 c          669 g          521 t
ORIGIN      
        1 aggccaaggt ctgtcgtctc aactccttga tccccttcgc ccccgcgccg gacaccgcct
       61 gatagctcga ggcgatgacg cgcctgaccc ggccatagtc gtgcagcggc ttgagcggca
      121 tgagcatgac gatcgtggtg cagttggggt tggcgatgat accacgagcc tgatacccgg
      181 caatcgcgtc cgggttgatc tccggaatga cgagtgggat atcggactgc atccgaaacg
      241 cggagctgtt atcgatgacg acggcgccag ccttgacagc cgcaggggcg aactccagac
      301 tgcgggtggc gccggccgaa aagagggcga tctcaatccc ctgaaacgag ccatcgccca
      361 ggttctcaac cttgatctcc tcccccttaa aggtgaggct cttcccggct gagcgctctg
      421 aggccaaaag cctcagacgc cggacgggaa agttccgctc ttccagcagt cgaagcattg
      481 tctctccgac cgcgccggtg gccccggcga ccgctaccgt gtatgttctg ttcgccatgt
      541 cacgtcctcg cggtcacagt ccccttggcg cggccatatc tttggggggc cgcgccgcgc
      601 cgacaatctt attgatcgcg ttcaggtaag ctctcacgct agcctcgatg acatcagttg
      661 aggttgctct gccgatcacg gtatggccat tgacttccag cttcaggacc acttcgccca
      721 acgcgtcctt ccctgcggtg atggccctga tcgagtagtc agccagccgc ccctggacct
      781 tcgtgatctg atcgatcgct ttgtaagccg cgtccaccgg gccatcgccc cagcctgatt
      841 cctggaagat ctcgttctcc cgcttgagcc gcacggtggc cgtggggacg atgttgatac
      901 cgctcgtgaa ctgcagatac tcgagggtat aggtctcgcc ggcagccagg gcatcctcct
      961 cgacgatggc cagcaggtcg tcgtcgaaca cctctttctt cttatcggcc acaaccttga
     1021 accgggtaaa ggccctgttt aaggcgtctt caggcaacat cacgcccagc tcctccagcc
     1081 gcttcttgaa ggcgtgccgc ccggagtgct tgccaaggac cagtcggctc tgaggcaccc
     1141 ccaccgattc cggggtcata atctcatagg tgagcggctt ctgcagcatg ccgtgttggt
     1201 gtacgccggc ctcgtgcgcg aaggcgttgg cgccgacgat ggccttgttc ggctggacgg
     1261 ggatcccgat gatgctggtc aggagcttgc tggaccggta gatctcctcc gtgttgattc
     1321 cggtgtcaaa gtcgagcagg tcttttcgtg tccgcagcgc catcacgatc tcttcgagag
     1381 aggcatttcc ggcgcgctcg ccgattccgt taatggtgca ttcgatctgt ctggccccgt
     1441 ttctgatcgc ctccagcgag ttcgccaccg ccagtccaag gtcgttatga cagtggcagc
     1501 ttatcaccgc cttatcgata ttgggcacac gatcgaacag attcttgatc cgggctccaa
     1561 actcccaggg gaggccatag ccgaccgtat caggaatgtt gatcgtggtg gcgcctgcct
     1621 tgatcacctc ttccacaacc ttacagagat agtcctgctc gctccggtgg gcatcctccg
     1681 gagagaactc gacatcgtcg gtatagcgcc tggcatgctt gaccgccgca atggcggcct
     1741 gcatgacctc ttcctgagtc gatttcagct tgtacgtgat gtgaatctcg gaggtggcga
     1801 tgaagatatg gatccgtggg cgatcagcgt atttcaacgc ctcccaggcg cggtcgatgt
     1861 cgatatcgcg agtccggcag aggctggcaa tgatcggccc acccttcagg ttctgggcga
     1921 tcgtcttgac cgcctcaaag tcgtcttcgg aggcgatggc gaaaccggcc tcgatcacgt
     1981 ccaccttcag acgcgcaagt tgccgggcca tctccagctt ctccctggta ttcatgctgc
     2041 agcccggcgc ctgctcgcca tccctgagcg tggtgtcgaa gatcagaatc cgattgccca
     2101 tcttatcctc tagggtatcc ctttatattc cgttcgtggt gagctggtcg aaccatgaac
     2161 ggaacttgtc gaaatgcttc acccttcgac cagctcaggg cgaacggaat gtaaagaagc
     2221 gttaggcgca cctggttatt ttccgcagat agcacaacga atcataacga ccagagcagt
     2281 agtgcgacga tgagactcgc gatgacgatc gcgacgatgg ccatggttct tgtcaatctc
     2341 gattg
//
LOCUS       DATMXI010000243         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_67930, whole genome shotgun sequence.
ACCESSION   DATMXI010000243 DATMXI010000000
VERSION     DATMXI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67930"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VN444_04625"
     CDS             complement(<1..643)
                     /locus_tag="VN444_04625"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWR21130.1"
                     /translation="MGYCNRVGRRSVLLLVAVSLLGLGSPLRTYGFTIISEAEENEIG
                     RKADQEILGQFGRYRDQQLQAYVESVGQRLLDGIGPTSFHYSFKIVDIPEVNAMALPG
                     GYIYITRGMLAMLNSEAQLAGVLGHEIGHATSRHAAHQLTKALGAQILSLGLMAASPG
                     GRQNTGEWARVSGAIFDHILLGYGREAEMEADELGLRTAYRAGYDPREMVIFLK"
     gene            complement(669..1124)
                     /locus_tag="VN444_04630"
     CDS             complement(669..1124)
                     /locus_tag="VN444_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21131.1"
                     /translation="MVALGPYSWNRCVKKLHLIGLAATLVVLVLQGASFALEGKSSET
                     DGKPAANRTTTAEAHYELGLSYHERMFADLNQAIAEYEQAIKLRSDFAEVHYHLGLSY
                     HTKAKLGSDDKVLYRNALKEYKLYLKYLPKGPLAEKARQNIKVVESRLR"
     gene            complement(1306..1381)
                     /locus_tag="VN444_04635"
     tRNA            complement(1306..1381)
                     /locus_tag="VN444_04635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:cgt)
     gene            complement(1421..1840)
                     /locus_tag="VN444_04640"
     CDS             complement(1421..1840)
                     /locus_tag="VN444_04640"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWR21132.1"
                     /translation="MAKQVLDHLTGRRRFSGRINLSCLTPFQQRVLRKIRTIPTGQVR
                     SYQWVAKEIGAARAARAVGTALAKNPIPLLIPCHRVVRSDGRLGEYSGGGPSVKAKLL
                     AFEGVDLENLTRLRIRREIAIGRRRKGGTDRPMSSVA"
     gene            complement(2068..3192)
                     /locus_tag="VN444_04645"
     CDS             complement(2068..3192)
                     /locus_tag="VN444_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWR21133.1"
                     /translation="MPEQIYSASRLSTYESCPLQYRFRYLDRIPREEESIEAFLGSRV
                     HEVLHKLYRDLQPGEDPSLADLLNDYHGQWGQHWHDQVKIVKQDYSIDYYKQFGERCL
                     GNYYQAHKPFNHGQVLGLEYQVATSLDPNSCYNIQGYIDRLVRIGPGCYEIHDYKTSG
                     RLPNQEKIDVDRQLALYQLAIEGMWPDAKEIELVWHYLAFGRERRSRRTPTALESLKS
                     STIALIDRIEADTEFKPIKTNLCHWCAYKNVCPLWNDHLDSTHAQSVTASGNGRSAEV
                     QTVVDLGGIDLQSISCDALREAIVMAAEHEGTEIRFAEHGQLWICASQLQLRANLDST
                     VDGWVRLSSESLVMLFRQVELARLSAPFVEKLQRYLQTRV"
     gene            complement(3185..3862)
                     /locus_tag="VN444_04650"
     CDS             complement(3185..3862)
                     /locus_tag="VN444_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR21134.1"
                     /translation="MQPTLYLKQVEVGQMANYVYLIGSTKTREVAVVDPAWEIDRIVE
                     LAQADEMSITHILATHTHPDHVGGKLYGLQIQGIAELLERVDAKVVVHRAEADHLTPL
                     AGSNIMRVDHGDTLNLGDLTMTMIHTPGHTPGSQCFLVQDRLFSGDTLFIGACGRVDL
                     PGSSPDQMYDSLTNRLMKLDDKTVLLPGHNYAAGRTSSSIGEERTRNPYVQCRSLAEF
                     LRTVGYA"
     gene            complement(3924..>4525)
                     /locus_tag="VN444_04655"
     CDS             complement(3924..>4525)
                     /locus_tag="VN444_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR21135.1"
                     /translation="TVEAVERLGQRAFAIQADLAEPDQVEALADYAVQACGKIDVLVN
                     SAAVFRMTPLESLTAQDWEQFLRVNLTGPFLLARRLGLLMRRQGAGKIINVADVAGIK
                     PWANFLPYSISKGMLITITQGLAKALAPEVQVNAVVPGTVLLSEDYGEKERESIVKGT
                     LLKRIGDPVDIARTVLFLVEGSDFITGQVVVVDGGQSIR"
BASE COUNT          950 a         1363 c         1248 g          964 t
ORIGIN      
        1 ctttaaggaa gatcaccatt tctctgggat cgtatccagc gcggtaggcc gtgcggagcc
       61 ctagctcgtc agcttccatc tccgcctctc gaccataccc caagaggatg tgatcgaaaa
      121 tggctcccga caccctcgcc cactcccctg tattctgacg accgccaggg ctcgcagcca
      181 taagacccag cgacaggatt tgcgcaccca atgctttggt aagctgatga gccgcatggc
      241 gagaggtggc gtggccgatc tcatgaccga ggaccccggc gagctgcgcc tcgctattca
      301 gcatcgccag catccctcgt gtgatgtaga tgtagccgcc gggcagggcc atcgcgttta
      361 cttccgggat atcgacgatc ttgaagctat agtggaaact ggttgggcca atcccgtcga
      421 gcagtcgctg accgaccgac tccacatacg cttgcagttg ctgatcgcga tagcgcccaa
      481 actgcccgag gatctcttga tccgctttcc tgccaatctc gttctcctca gcttcagaga
      541 taatggtaaa accataggtg cgtagagggg aaccgagtcc aagcaaggac accgcgacca
      601 acagcaacac actacgccta ccgaccctgt tgcagtagcc catcactcac aatcctcata
      661 ccgaacgctt atcgaagcct cgactccact accttaatgt tctgccgggc cttttcagcc
      721 aatggaccct ttggaaggta cttcagatac agcttgtatt ctttcagcgc attgcgatac
      781 aggaccttat cgtcactacc caatttggcc ttcgtatggt acgagagccc cagatggtag
      841 tgtacctcgg cgaagtcgct ccgaagcttg atcgcctgct cgtactcggc aatcgcctga
      901 ttgaggtcgg cgaacattcg ctcatggtaa gaaagcccga gttcgtaatg cgcctctgcc
      961 gttgtcgtcc gattagcagc cggcttgcca tcggtttctg acgatttgcc ctcaagggcg
     1021 aaacttgccc cctgaaggac aagcaccacc aacgtcgcag ccagaccaat gaggtgcagc
     1081 ttcttcacgc aacgattcca gctatagggt cccaatgcga ccattttgcc tcgtagtgtc
     1141 agagcgatta ggaaatgcga tcgacaggta acgatctata gcctctgcaa cacgcctcgc
     1201 gatggcatca tatcgtggta ggattgctga atctcttgaa ggcagaaaat gcgcgatccc
     1261 gttttgcggc gatacgctca gcgtgcaatg cgccgatcaa acggctggag ccggcgatgg
     1321 gaatcgaacc cgcgacctgc tgattacgaa tcagctgctc taccaactga gctacgccgg
     1381 cgcgagggtg caactactat agccaagggt ctcagggctg tcaagctaca gaagacatgg
     1441 ggcgatccgt gccgccctta cggcggcggc cgattgcgat ctcgcggcgg atccgcaggc
     1501 gcgtcaggtt ctccagatcg acgccttcaa aggccagcag cttcgccttg acggaagggc
     1561 cacctccgga atactccccg aggcggccat cgctcctgac cacccggtgg catgggatca
     1621 gaagcgggat cgggttcttg gccaaggctg tcccgaccgc acgggctgcc cgtgccgcgc
     1681 caatttcctt tgcgacccat tggtatgagc gcacctgacc ggtggggatt gtccggatct
     1741 ttcgcaagac cctctgctgg aaaggtgtga gacaggacag gttgattcga ccagagaaac
     1801 gccgccgccc tgtcagatga tccagtacct gtttggccat gtctcccggt aggcggggat
     1861 cccgtatcgg tcgaatgccg aatagcctct cgcatacccg ctcaaagcca cgcccatccg
     1921 acccgagggc aacacagcac acagccttgc cacgataggc cacataaagc cgtttaagcg
     1981 gcgttgaaac cgatgcgtac cgcataaggc gagaagtgcg acacatgagg ttcatgatgt
     2041 agacgacggc gcgctatgtt ctttacctta taccctggtc tgcaggtacc gctgtagctt
     2101 ttcaacaaag ggggcactca gccgcgcgag ttccacctgc ctaaagagca tgactaagct
     2161 ttcagaggac agtctgaccc acccgtccac cgtactatcc aggttggccc tcagttgtaa
     2221 ttggctcgca cagatccaga gctgaccgtg ttcggcaaaa cggatctctg ttccttcatg
     2281 ctcggccgcc atcacgatcg cctcccggag cgcgtcgcaa gaaatagatt gcaggtcaat
     2341 ccccccaaga tcgaccacgg tctggacctc cgccgatcgc ccattgccgg aggccgtgac
     2401 agattgggca tgcgttgaat cgaggtgatc attccaaagc ggacagacgt tcttgtaggc
     2461 gcaccaatgg caaaggttgg ttttgattgg cttgaactcg gtatcggcct caatccggtc
     2521 gatgagggcg atcgtcgaac ttttcaggct ctcgagcgct gtcggtgttc gccttgaacg
     2581 gcgctccctt ccgaaagcca ggtagtgcca gaccagctcg atctctttgg cgtcaggcca
     2641 catcccttct atcgcgagct gatagagggc tagttgccga tcaacgtcga tcttttcctg
     2701 gttcggcaga cgaccggacg tcttgtagtc atggatctcg tagcatcctg gcccgatcct
     2761 caccagacgg tcaatgtatc cttgaatgtt gtagcagcta ttggggtcaa gcgaggtggc
     2821 cacctgatat tccaatccca atacctgtcc gtggttgaac ggcttatgag cctgatagta
     2881 gttacccaga caacgctcgc caaactgctt gtaatagtcg atcgaatagt cctgtttgac
     2941 gatcttcacc tggtcgtgcc agtgctgccc ccactgcccg tgatagtcgt tcagcagatc
     3001 agcgaggctc ggatcttcac cgggctgcag gtctcgatag agcttatgca gcacctcatg
     3061 cacccgtgat ccgaggaatg cctcgatcga ctcctcctca cgggggatcc gatcgaggta
     3121 gcggaacctg tactgaaggg ggcaggactc gtaggtggag agccgggatg ctgagtagat
     3181 ctgctcaggc atatcccacg gtgcgaagga actcggcgag agagcgacac tgaacatagg
     3241 ggttccgcgt ccgctcctcg ccgatgctgc tggaggtgcg gccagcggcg taattatgac
     3301 cgggcaggag tacggtctta tcgtccagct tcatcaatct attggtcagc gaatcgtaca
     3361 tctgatcggg gctgctacca gggagatcca ctcggccgca cgcgccgatg aatagcgtat
     3421 cgcctgaaaa cagccggtcc tgaaccagaa aacattgtga cccgggcgtg tggccgggag
     3481 tatggatcat cgtcatggtg aggtcgccaa ggttcagggt gtcgccatga tcgacccgca
     3541 tgatgttcga tccagcgagg ggggtcaggt gatccgcttc agctctatga acgaccacct
     3601 tggcgtctac ccgctcgagc aactccgcga ttccctgaat ctgaagacca tacagcttcc
     3661 cacccacatg atcgggatgg gtatgggtgg cgaggatgtg ggtgatgctc atctcatcgg
     3721 cctgggcaag ttccacgatc ctatcgatct cccaggccgg atcgaccaca gcgacctccc
     3781 tcgtcttggt cgagccgatg agatagacgt agttggccat ctggcctact tcaacttgct
     3841 tcaggtagag ggttggctgc atgctcatag cgtccatagc gtcgttgcgt ctgtagcgtc
     3901 atcgcgtaga cgcaatagac gcattaccga atcgattgac ctccatccac taccacgact
     3961 tgaccggtaa tgaaatccga gccctccaca aggaagagga cggtcctggc aatgtcgacc
     4021 gggtcgccga tccgcttgag cagcgtgccc ttgacgatcg actcccgctc cttctcgcca
     4081 tagtcctccg acagcagcac ggtaccaggc accacggcgt tcacctggac ctccggcgcc
     4141 agcgccttcg ccagcccttg ggttatggta atcaacatcc ctttggagat ggaatagggt
     4201 aggaagttgg cccacggttt gatccctgcg acatcggcga cattgatgat cttccccgct
     4261 ccttgccgcc gcatcagcag gccaagtcgt ctggccagca ggaacggtcc cgtgaggttg
     4321 acgcgcagga actgttccca gtcctgcgcg gtgaggcttt caaggggcgt catgcggaat
     4381 acggccgcgc tgttcaccag gacgtcaatc tttccacacg cctggactgc gtagtcggcg
     4441 agcgcctcga cctgatccgg ctccgccaga tcagcctgga tagcgaatgc tcgctggccg
     4501 agacgctcca ccgcctctac cgtct
//
LOCUS       DATMXI010000244         5835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_7198, whole genome shotgun sequence.
ACCESSION   DATMXI010000244 DATMXI010000000
VERSION     DATMXI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7198"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VN444_04660"
     CDS             <1..269
                     /locus_tag="VN444_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWR21136.1"
                     /translation="LLHPRTGMPANSALSATIIAKSAMRADGLATAAMIHGAGAMAFI
                     QSVSGVEGIVVHPLARHPGKVSVQITPGLRGRVELVDRSAELER"
     gene            315..1754
                     /locus_tag="VN444_04665"
     CDS             315..1754
                     /locus_tag="VN444_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21137.1"
                     /translation="MKVLRCLIAALMVVIASSSMAIAATLGDLEGQLKAIQSEIERLK
                     QDKQAQDQRVRELEAAQKEQAVKEAQAQQEQARRREEQERKTVILSEEMEALRSKFFL
                     PETVEYKSLYGLGPAASKVYQMPRGLSIGGYGEANANFFVGDNEGPGKKRDVGDLLRF
                     VTYIGYKFSDRIILNTEIEYEHAKVQPTTSSSGGDVEIEFATLDFLLTERANIRAGLL
                     LMPMGFINEMHEPTTYFGNLRPEVEQRIIPSTWRELGVGLHGTILPGLTYRTYVTNSL
                     NAKGFSKTNIRGTRQNGNRALFEDFAWTTRLDYTPTPGLLLGGSIFWGDTGQDQLFDG
                     KEIAANLRLYEFHGQYQYRGLQLRALFAQGHISDADVLSAELKTPISRRIVGGYAEAS
                     YDILPLVFPGTDMSLSPFIRFERLDTQADVPSGFTADKSKDIRVINAGLSFKPIPNVV
                     LKLDYRNLDGKGKEGQIADELNVGLGFIF"
     gene            1863..2411
                     /locus_tag="VN444_04670"
     CDS             1863..2411
                     /locus_tag="VN444_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWR21138.1"
                     /translation="MRALAGLTFLLVLHLCAPVGLLAKVFYAKDEAVRAAFPEADVIE
                     KQTFFLTDDQKKQIETLARTPLDSKLVTMYIGKRGQKLLGYAMIDVHTVRTLPEAVMV
                     VLSPDGRVASTLILAFYEPLEYLPNERWLKQFDQARLTPDLRVGGRIAGITGATLTAR
                     AMTESVRKVLALYQILIEKGGR"
     gene            2411..2920
                     /locus_tag="VN444_04675"
     CDS             2411..2920
                     /locus_tag="VN444_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013817298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21139.1"
                     /translation="MRFVITGEWTQNRLLRLIIVWFLFYSAGLWLTNALLFVNQMGLR
                     YESVVAFYRGSEAQFLLPRSYKVLLEISHFHLLAMGIFILTLSHLVLFVPFTPRVKYW
                     LIHLTFLSAISDEAAGWLTRFAHPMFAYVKIGAFILLQTTMAVLIIAVLLAVCRKARS
                     AYTESGGIS"
     gene            2917..4068
                     /locus_tag="VN444_04680"
     CDS             2917..4068
                     /locus_tag="VN444_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWR21140.1"
                     /translation="MNNSRQSFACYRRGQYQGLKVLLLLFVTMLIGCTSALAQQTRTY
                     HIAAIEVDWDYAPTGINQISGNAFGESENVFVQGGKERIGKVYQKAAYREYTDGTFTT
                     PKTVEPKWQHLGIMGPVIRGEVGDTIQVIFKNMTSFPNSMHPHGVLYAKSSEGAPYND
                     ATSGSDKADDAVPPGGTHTYIWQVPERSGPGPMDASSVLWMYHAHTDEPKDTNTGLVG
                     PLIITRQGSAYADGTPKDVDREFVTLFTVFDENASHYLEQNITRFTSKPRRVLKKRLE
                     DPEFQESNLMHSVNGYVYGNLPGLTMKKGERVRWYLLSIGTEVDLHTPHWHGQTVLMM
                     GMRMDMVELLAGSMKTVDMAPDNPGTWLYHCHVNDHITAGMMALFTVTE"
     gene            4636..4902
                     /locus_tag="VN444_04685"
     CDS             4636..4902
                     /locus_tag="VN444_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21141.1"
                     /translation="MRKYEKKAQVLLTERQYRDLQAIAEQLQKPLGALLRDAAEEVYL
                     KQKRSHDKAQAVQALLSLTETEVPDEYHDWEEQYLSRKASHGSD"
     gene            4889..5326
                     /locus_tag="VN444_04690"
     CDS             4889..5326
                     /locus_tag="VN444_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWR21142.1"
                     /translation="MAATETLFLDTNIIMYAIGTDHPLKPACVAALRQVEAGTIQVVT
                     NVEVFQELLHRYTAIGRHEVAAHAFTHLKALCEQVLPVLEADLDRAFAILNDHPHISV
                     RDALHAATMLNNDLRTILSTDTHFDAIPEITRIDPRTLLFPNN"
BASE COUNT         1324 a         1714 c         1624 g         1173 t
ORIGIN      
        1 atctgttgca cccccggacc ggcatgccgg cgaacagcgc gctgagcgcg accatcatcg
       61 caaagagcgc catgcgcgcc gacggcctgg cgaccgccgc catgatccac ggggcaggcg
      121 cgatggcgtt catacaatcg gtgagcgggg tcgaagggat cgtggtccac cctctcgctc
      181 gccatccagg gaaggtctcg gttcagataa cgccgggact tcgtggccgg gtggagcttg
      241 tggatcgctc ggccgagctt gaacggtaat ctactcgtac aaaccggtaa ccaaggaatc
      301 gaaaggagaa ggagatgaag gtgttgcgat gtctcatagc tgcattgatg gtcgtgatcg
      361 cctcgtccag catggcgatc gcggcgacat tgggtgatct ggaggggcaa ctgaaggcga
      421 ttcagtcgga gattgaacga ctcaagcagg acaagcaagc acaggatcaa agggtacgtg
      481 agctcgaggc cgctcagaag gagcaggcgg tcaaggaggc ccaggctcaa caagagcagg
      541 cgaggcggcg agaagaacag gagcggaaga cggtgatcct ctcggaggag atggaggcgc
      601 tcaggagcaa gttcttcctg cccgaaacgg tcgagtacaa gagcctctac ggcctgggtc
      661 cggcggcctc gaaggtctac cagatgccga gagggctgtc aatcggtggc tacggcgagg
      721 ccaacgccaa cttcttcgtc ggcgacaatg aggggccggg caagaagaga gatgtcggcg
      781 atctcttgcg cttcgtcacc tatatcgggt acaagttctc cgaccggatc atcctgaata
      841 cggagatcga gtacgagcat gcgaaggtcc aacccacgac gagctcaagc ggtggggatg
      901 tcgagatcga attcgccacg ctggacttcc tcctgactga gcgggcaaac atcagagcgg
      961 gactactcct gatgccgatg ggattcatca acgagatgca tgagcccaca acttacttcg
     1021 gcaacctccg cccggaggtg gagcagcgga tcatcccgag cacctggcgg gagcttgggg
     1081 tagggctcca cggcacgatt ctgccggggc tgacctaccg gacctatgtc acaaacagtc
     1141 tgaacgccaa gggcttttcc aagaccaata tccggggcac ccggcaaaat gggaaccgag
     1201 ccctgtttga ggacttcgcc tggaccacgc gcctcgatta caccccgacg ccgggtctcc
     1261 tgctcggagg gtcgatcttc tggggcgaca ccggacagga tcaactgttc gatgggaaag
     1321 agatcgctgc aaacctcaga ctctacgagt tccatggcca gtatcagtat cgagggctgc
     1381 aactacgcgc actgttcgcc caggggcaca tcagcgacgc cgatgttctg agcgccgagt
     1441 taaagacacc catcagccgg cgtatcgtag ggggttacgc ggaggccagc tatgacattc
     1501 ttccgctcgt cttccccggg accgacatgt cgctgtctcc gttcatccgg ttcgagcggc
     1561 tcgacaccca ggccgatgtt ccctccggct ttaccgcaga caaatcaaag gacatcagag
     1621 tgatcaacgc tgggctaagc ttcaaaccga tccccaacgt ggtgctcaag ctggactacc
     1681 ggaacctcga tgggaagggg aaggagggcc agatcgccga cgagctcaac gtcggcctcg
     1741 gcttcatctt ctagccggaa ccgggcaacc taaagagtgg tgtgaggcgg gggctcagga
     1801 gcatccgtaa ggtcgtccga gccctcgccc tcatgtctcc aaccctatca aggagaaagg
     1861 ctatgcgtgc cctggcgggt ctgaccttcc tgctcgtctt gcacctctgc gcacccgttg
     1921 gcctcctcgc caaggtgttt tacgccaagg acgaggccgt ccgggcggcg ttcccggagg
     1981 ccgacgtcat cgagaagcag acattttttc tgacagacga ccagaaaaag cagatcgaga
     2041 ccctcgcacg cacgccactg gattccaagc ttgtgacgat gtacatcgga aaacgaggtc
     2101 agaagctact cggctatgca atgatcgacg ttcacacggt acggaccctg ccggaggctg
     2161 tcatggttgt gctctctccg gacgggcggg tggcctccac gctcatccta gccttctatg
     2221 agccactgga atacctccca aatgaacgct ggctcaagca gttcgatcag gcaaggctca
     2281 ccccggacct ccgggtggga ggaaggatcg cgggaatcac cggggcgacc ttgacggctc
     2341 gggcaatgac cgagtcggtc cgtaaggttc tggccctcta ccagatcttg atcgaaaaag
     2401 gagggcgctg atgcgcttcg tcatcacggg agagtggacc cagaaccgcc tccttcgtct
     2461 catcattgtg tggtttctct tctacagtgc aggcctttgg ctgaccaacg cattactctt
     2521 cgttaatcag atgggacttc gttacgagtc cgtcgtcgcc ttctaccggg gatccgaggc
     2581 gcagtttttg ctgccacgca gctacaaggt cttgctcgag atcagtcatt tccatctcct
     2641 tgcgatgggc atcttcatct tgacgctgag tcacctggtg ctgttcgtgc cttttacgcc
     2701 tcgagtcaag tactggctga tccacctgac cttcctatca gcgatttcag acgaggccgc
     2761 aggctggcta acccgattcg cacatcccat gttcgcctac gtcaagatcg gagccttcat
     2821 tcttcttcag acaaccatgg cagtactgat catcgctgtc ctgctcgccg tgtgccggaa
     2881 ggcacgcagc gcatacacag aatcaggagg gatatcatga acaactcgcg acaatcgttt
     2941 gcctgctacc ggcggggtca atatcaggga ttgaaggttc tcttgctact gttcgtcacg
     3001 atgctgatcg ggtgcacatc cgctctagcc cagcagaccc gcacctatca catcgcggct
     3061 atcgaggtgg actgggatta cgcgccgacc ggtatcaacc agatcagcgg gaacgcgttt
     3121 ggtgagagcg aaaatgtctt cgttcaaggt ggcaaagagc ggatcggcaa agtgtatcag
     3181 aaagctgcct accgcgagta cacggatggg accttcacaa cacccaagac tgtcgagccg
     3241 aaatggcaac acctgggtat catgggtccc gtcatccgag gggaggttgg ggataccatc
     3301 caggtcatct ttaagaacat gaccagcttc cccaacagca tgcacccgca cggcgtcctg
     3361 tatgcaaaga gttccgaggg ggcgccgtac aacgacgcca cgtcaggatc cgacaaggcc
     3421 gacgacgctg tgccgccggg cggtacgcat acgtacatct ggcaggtccc ggaacggtct
     3481 ggtccaggcc caatggatgc cagttccgtc ctttggatgt atcacgccca caccgatgag
     3541 ccgaaagata caaataccgg gttggtcgga ccactcatta ttacacgtca aggaagtgct
     3601 tacgctgacg gcacaccgaa ggacgtcgat cgagagtttg tcacgctgtt caccgtcttt
     3661 gatgaaaatg ccagccatta tttggagcag aacattacac gattcacttc caagccgcgt
     3721 agggtgttga agaagcggct ggaggaccct gagtttcagg aaagcaacct tatgcattcg
     3781 gtcaacggct acgtctatgg caacctgcct gggttgacca tgaagaaggg cgagcgagtg
     3841 cgctggtacc tactcagcat aggaacagag gttgatctgc acacgccgca ctggcatggc
     3901 cagaccgtgc tgatgatggg aatgcgcatg gacatggtgg aactgctggc tgggagcatg
     3961 aagacggtag acatggcgcc ggacaacccg ggtacctggc tgtatcactg ccacgtgaac
     4021 gaccacatca ccgcggggat gatggcgctc tttacggtca ccgaatagta agaacactga
     4081 ggaggctgtg agctttcggc taacggctga gtgccgacta ttagaagcta tttttgagca
     4141 cacttgtatg aaatgtgggg aaagggctgg gagcaaccga ctgaggagag gatcggaagg
     4201 gggtatccac ttgacgcaag tcggcccaag catttgtggt gtagaggcat caatccccac
     4261 atgatcttgt ggttactgga gtgaagtgga gacccccttg atcggaatac gcacaagtcg
     4321 tgcgtcaaga gttttggcgt catgatgacg ccctaaatac ggagcagcca gcgcgtcccc
     4381 tatctgacac gattaacccg ccttacaagt cgataactca cggatttttc agagaaaatc
     4441 ctgccatatc ctcagcctca gttccaatcc tttggacggc gtataatagc cactatacgc
     4501 cgcccagacc gccacgcatg cgtttccaca cagatattcc caacctcata cgcttggtca
     4561 gatactctct agagtattca agagtcttga cagacaaatc atctatgcga tacagtctct
     4621 atgaggttat gtctgatgcg aaaatatgag aaaaaagccc aggtcctcct gacggaacgg
     4681 cagtaccggg atctccaggc gatcgccgaa caactgcaaa aaccgcttgg cgcgttgctt
     4741 cgcgatgccg ctgaagaggt ctatctcaaa cagaagcgct cccacgataa ggcccaggcg
     4801 gtccaggccc tcctctctct caccgaaacc gaggtaccag acgagtatca cgactgggag
     4861 gagcaatacc tctctcgcaa ggccagtcat ggcagcgact gagactctct tcctcgacac
     4921 caacatcatc atgtacgcca ttggcacaga ccatcccctc aagcccgcct gtgtagcggc
     4981 actgaggcag gtcgaggccg ggaccatcca ggtcgtcacc aacgtggagg tcttccagga
     5041 gctgctgcac cggtacactg ccattggacg ccatgaggta gccgcccacg ccttcaccca
     5101 cctcaaggcc ctgtgtgagc aggtcctccc tgtcctcgaa gccgatctcg accgtgcctt
     5161 cgcgatcctt aacgaccacc cccacatcag cgtccgcgac gctctccatg cagcgaccat
     5221 gctcaacaac gacctacgca cgattctgag caccgacacg cacttcgatg ccatccccga
     5281 gatcactcgg atcgatccac gcaccctcct cttccccaat aactgatcac gcccaactct
     5341 aactggctag agacactctc cgccttcgaa cgctagccca aggccaaagc aggcgacccc
     5401 tcaccattaa atcgcaccga tacaacatcg cgctgcttgc atagggataa tagggacaga
     5461 cactatttat tgggctcctt tcttggtctt cccctctgca acacgtgtat tactcgttct
     5521 gtttcctgcg cacttcccaa atgaacccat ccctccccag cagtggtcgc tctgtcatcg
     5581 tgtgccttcg aatcgctgtc gttcgaaaga ccgcccggtg atcatgccct cgttgcctca
     5641 catgctacgg gtggcctggt gcaacagtgc gggtaatccg tggcatacac gagcatgacc
     5701 gtacacacca cgaatcgtca cgataatagt gtctgtccct attttcccta ttttccctat
     5761 tttctcggaa tctctgtggg tcctggagca gtgcttcgaa gattcgcccg ctcatgagcg
     5821 aacggatgtc ccggt
//
LOCUS       DATMXI010000245         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_198285, whole genome shotgun sequence.
ACCESSION   DATMXI010000245 DATMXI010000000
VERSION     DATMXI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198285"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VN444_04695"
     CDS             complement(<1..1434)
                     /locus_tag="VN444_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWR21143.1"
                     /translation="MGWIQDLFKPERREWEDFYRNRWQYDRIVRSTHGVNCTGGCSWE
                     VYVKDGIIVWEMQQIDYPMLEDKLPPYEPRGCQRGISASWYVYSPIRVKYPYIRGPLL
                     DFWREAKQQHENPVEAWRSIVEDEPKRKRYQRARGKGGFRRASWDEALEIIAAASLYT
                     AKKYGPDRLFSFSPIPAMSYLSYAAGSRFTQLFGGASMSFYDWYADLPNQFPETWGDQ
                     TDVCESADWYNSKFIVSVGANLNMTRTPDVHFISEGRYEGAKFVVVAPDFSQVAKYSD
                     WWIPVKAGEDNAMWMAVNHVILKEFHADRTIDYFIDYLKRYSDSPFLVKLDKESDGSV
                     KPGRLLTAGEIERYGSEEHNAWKFLIQDSLSDELRCPKGSVGDHWGSRKGRWNLKLED
                     SLDDSRIDPQLSFLNQCDEVMQVKVYDFAGKRYLLRGVPVRHVPTKDGPAPVATVYDL
                     LFAHYGVSRGLPGDYPNGYDDTAPYTPA"
     gene            complement(1434..>2552)
                     /locus_tag="VN444_04700"
     CDS             complement(1434..>2552)
                     /locus_tag="VN444_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWR21144.1"
                     /translation="AQECAACHGADGRGKGKAADLLEIKPRNFVTAKYRMVSTWERLP
                     TDEDLFNAITRGLPGTAMPSWEHLPELQRWALVQYVKSFAETPIAIKPQKAPAPDASG
                     GEGIIAVPPESPYDPVVAKRAKELYADGCASCHGPTGKGDGVQEQLDEDGVPTHPRDL
                     TEGVFKGNPDPETLYRRILAGMPGTPMPMSDWAVGKDAWDLVHYVLSLSTPEQRDKAN
                     TWTPPEITGLMRGDPDKGKSLFTGAERLAGGGASCRACHSVSGIGALGGGVLGPDLSG
                     AYHKFGDGMITWTEHVAPMKLIYTKKTLTDEEKRHLLAFFTTGIGRATNQIYQLAGIG
                     AVGAVVFLLMIQGIWRNRGRDVRRTMYERSYIDQGGVS"
BASE COUNT          447 a          805 c          759 g          541 t
ORIGIN      
        1 ggcgggcgtg tacggcgcgg tgtcgtcgta cccgttgggg taatcgcccg gcagccctcg
       61 cgagacgcca taatgcgcga acagcaggtc gtagacggtc gcgaccggcg ccgggccgtc
      121 ctttgtgggc acatggcgga caggcacccc gcgcagcaga taccgctttc cggcaaaatc
      181 atagaccttg acctgcatca cctcatcgca ctgattgagg aagctgagct gcgggtcgat
      241 ccgcgagtcg tcgagtgaat cctccagctt gagattccat ctgcctttcc tgctgcccca
      301 gtggtccccg acgctgccct tcggacagcg caactcgtcc gaaaggctat cctgaataag
      361 aaacttccag gcgttgtgtt cttcgctgcc gtaccgttcg atttcccccg cagtcagcag
      421 gcggccgggt ttgacgcttc catccgattc cttgtcgagc ttgacgagaa acggactatc
      481 tgaatagcgc ttgaggtaat cgatgaagta gtcgatcgtt cggtcggcgt gaaactcctt
      541 gaggatgaca tggttgacag ccatccacat ggcgttgtct tcgccggcct tgacggggat
      601 ccaccagtcg gagtacttgg ccacctggct gaagtctggg gccaccacca cgaacttggc
      661 cccttcatag cggccctcag agataaaatg gacatcgggg gtgcgggtca tgttcaggtt
      721 ggcccccacc gacacgatga acttcgagtt gtaccaatcc gcgctttcac agacgtcggt
      781 ttgatcgccc caggtctccg ggaactggtt gggcagatcc gcgtaccagt cgtaaaagct
      841 catgctggcg ccgccgaaaa gctgggtaaa gcgcgagccg gccgcgtacg acagatacga
      901 catggccgga atgggcgaga agctgaacag ccggtccggt ccgtatttct ttgcggtata
      961 caggctggcg gccgcgatga tctccaacgc ctcgtcccac gacgcgcgcc ggaatccgcc
     1021 cttgccgcgc gctcgttgat accgtttgcg tttgggttcg tcttcgacga tggatcgcca
     1081 ggcttctacc gggttctcat gctgttgctt cgcctccctc cagaaatcga gcagggggcc
     1141 gcggatatac gggtatttca cccggatggg gctgtacaca taccaggagg cggagatacc
     1201 acgctgacat ccgcgaggtt cgtacggggg gagcttgtcc tcgagcatgg ggtagtcaat
     1261 ctgctgcatc tcccatacga taatcccgtc cttgacgtac acctcccagg aacatccgcc
     1321 ggtgcagttg acgccgtggg tgctgcgcac gatccggtcg tactgccaac ggttccggta
     1381 gaaatcctcc cactcgcggc gctcgggctt gaacaggtcc tggatccaac ccatcaggac
     1441 acccctcctt ggtcgatgta ggaccgttca tacatggttc ggcgcacatc ccggccccga
     1501 ttcctccata tcccctgaat catcaacagg aagaccacgg ctccgacggc accgatgccg
     1561 gcaagctgat agatctggtt ggttgcccga ccgatgcccg tggtgaagaa ggccaacagg
     1621 tgacgctttt cctcatccgt cagcgttttc ttggtgtaga tcagtttcat gggcgccacg
     1681 tgctctgtcc acgtgatcat cccatcaccg aatttgtgat acgctccgga caggtccggc
     1741 cccaggaccc cgcctccgag cgctccgatg ccgctgacgg agtggcacgc ccggcacgaa
     1801 gccccgccgc cggcgagtct ctcggcgccg gtaaacaggc tcttaccctt atccgggtcc
     1861 cccctcatca gccccgttat ctccggaggg gtccatgtat tcgccttgtc gcgctgctcc
     1921 ggcgtggaga gggacagcac atagtgaacc agatcccatg cgtccttgcc taccgcccag
     1981 tcgctcatcg gcatcggtgt tccgggcatt cccgcgagaa tccttcggta gagggtctca
     2041 gggtccgggt tccctttgaa gacgccctct gtcaggtcgc gcggatgcgt gggcacacca
     2101 tcttcatcca actgctcctg taccccatcc cctttgcctg tcggtccgtg gcaggaggcg
     2161 cagccgtcgg catagagttc cttcgcgcgc ttggcgacga cgggatcgta cggggactcc
     2221 ggcgggacag cgatgatgcc ttctcctccg cttgcgtcag gggcgggtgc tttctgcggc
     2281 tttatagcga tcggcgtttc ggcgaacgac ttgacatact ggaccaaggc ccagcgttgt
     2341 agttccggca ggtgctccca cgagggcatc gcggttccgg gtagcccgcg tgtaatcgcg
     2401 ttgaaaaggt cctcatcggt agggaggcgt tcccacgtgg ataccatgcg gtattttgcc
     2461 gtgacgaagt tacgcggctt gatctccagc aggtcggcgg ctttaccttt gccgcggccg
     2521 tccgctccgt ggcaggcggc gcactcctgg gc
//
LOCUS       DATMXI010000246         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_309923, whole genome shotgun sequence.
ACCESSION   DATMXI010000246 DATMXI010000000
VERSION     DATMXI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309923"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VN444_04705"
     CDS             <1..1316
                     /locus_tag="VN444_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWR21145.1"
                     /translation="RDALVPRVGSGMGWVYDHKTLHIRPDLQRDQVFLEDKWYVQEGL
                     GRMINLPLLVRDRCVGILNIGSIESGAPDPGDLEFLTQVAMQIAYAIDHVQAYEQIDR
                     LRDQLAKENVYLTEELKLMKNVGSLVGKSLAFRHMIGLARDVASTPTTVFVTGETGTG
                     KELVAQAIHDWSPRHLKPLVRVNCAAFPAGLVESELFGHERGAFTGADRAREGRFELA
                     HGGTLFLDEIGEMPLETQAKLLRVLQDGLVDRLGGKQPVRVDVRVIAATNRDLPAAVK
                     AGTFRADLFYRLNVFPINVPPLRNRPDDIPLLARHFLKQYGVKLGRSCKDIDEESLER
                     LIRYTWPGNVRELENVIERAMILSRDPLLRIDEHILGSQGAPSMVLPPPAGLKDLEQR
                     HILQTLTLTNWHIEGPDGAASRLRIPPSTLRSRMKQFGLKRPVSS"
     gene            complement(2046..>2840)
                     /locus_tag="VN444_04710"
     CDS             complement(2046..>2840)
                     /locus_tag="VN444_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase adenylyltransferase"
                     /protein_id="HWR21146.1"
                     /translation="VYGGSGNTNGKNEIENSEYFERLAQELLGWIEAKQEGIFRIDVR
                     LRPHGGKGALANAFEEICRYYSPAGLAAPFERQALIKLRHIAGNSTLGRKLETHRDSF
                     VYGDAPWDIPTALELRRQQLKQLVAPGKVNVKYSAGGLIDIEYTAQYLQVMHGHKHPG
                     LHTPNTLQALAALVNAGLIARTDGAGLRKAYIFFRMLVDGLRMVRGNTKDLVLPPPDS
                     EEFVSLARRIGYTRDDWRAGARELQSDIQQHMALTKTFYKRTFGAL"
BASE COUNT          614 a          750 c          792 g          684 t
ORIGIN      
        1 aacgagatgc ccttgttcca cgtgttggaa gcggtatggg ctgggtctac gatcataaaa
       61 ctctacacat ccgtcctgac ctgcagcggg atcaggtctt tcttgaagac aaatggtacg
      121 ttcaagaagg ccttgggcga atgatcaatc tgcctcttct cgtgagagac agatgtgtag
      181 gcattctcaa tattggcagt atagagtcgg gtgctcccga tccaggggac ctcgagttct
      241 tgacgcaggt ggccatgcag atcgcctatg cgatcgatca tgtccaagcg tacgaacaga
      301 tcgatcgatt gcgagaccaa cttgccaagg aaaacgtcta cctcaccgaa gaactgaaac
      361 tcatgaagaa cgtaggctca ttggtgggga agagcctggc ctttcggcat atgataggcc
      421 ttgcacgcga cgtggcgtcc acaccgacga ccgtgttcgt tacgggtgaa acgggaaccg
      481 gcaaggagtt agttgcgcaa gcgatccatg attggagccc ccggcacctg aaacccttgg
      541 ttcgggtcaa ttgcgccgct ttcccagcag ggttggtcga aagtgaattg tttggtcatg
      601 aacgaggcgc gttcaccggg gccgaccgcg cacgcgaggg ccggtttgag ctggcccatg
      661 gtggaaccct cttcttggat gaaatcggtg aaatgccttt ggagacacag gccaagttgt
      721 tacgagtcct acaagacggc ttggttgatc gtctgggtgg gaaacagccc gttcgggttg
      781 atgttcgcgt gatagccgcc accaatcgcg acttgcccgc cgccgtcaaa gccggcacat
      841 ttcgggcgga cctattttat cgtctgaacg tctttcctat caacgtgcct ccactcagga
      901 atcgccctga tgatatcccc ctactggctc gacattttct gaaacaatat ggcgtgaaac
      961 tcggccgttc gtgcaaggat attgacgagg aatctctaga acggttgatt cgctacacct
     1021 ggcctggcaa tgtccgtgaa ctggaaaacg tcattgagcg agcgatgatt ctttctcgtg
     1081 atcctctctt gcggatcgac gaacacatcc tgggttcgca gggtgcccct tccatggtgt
     1141 tgccaccacc cgccggtctc aaagatctcg aacaacggca catcctgcag acccttacgc
     1201 tgacaaactg gcacatcgaa ggtcccgatg gtgcagcctc gcgcctgagg attcctccca
     1261 gcacattgcg cagccggatg aagcagtttg gtctgaagcg gccggtttct agctgaagta
     1321 atagaataac cctctcctgt acgacctcgc gctccattcc ctcagaacga gcaacccacg
     1381 atctgtcgtg ggctgagccc acgacgggcc gcggcccgtc gtggaagtta gccgagcttt
     1441 agcgatgctc cttcatgtta tagaaaaact aataagaatc tcaatatgtt gagaattcta
     1501 tgctaaagga tgttgagcgg cacgccccgt gcaacacata cattcagcac gcagattcaa
     1561 ctcatttgaa aggagattta acatgaccgc tgctttttta atgaatggcg cactatatat
     1621 ttgtttgatt ggagttttgg caatcgcatg gggatggttg cagagagtgg atcctgagtg
     1681 agctagaaag cggggtatca cagtgggcgc gtctaaggag tctggataat acacgagact
     1741 ggtttctaaa gagatggagc gtcggatacg actttacggg cgcactgagt gtaccgatag
     1801 agtcatttga tgggcatcgc agggttcggt tactgctgac gaaactcgtg tcctccaaac
     1861 gtgctcgttt tttcttcagg gagggtggcc tgcttgatct ccccgtgtgc gcatcgaacg
     1921 agcacattcc tatcgtgcgc gttctgcgag caagaaggag accgtctggc tgctcccttc
     1981 ccacccttct gcggccgcgc gttgcgcgag cacaggagat ccacaggcca ctctcccgtt
     2041 ccccttcata acgctccgaa cgtccgtttg tagaaagtct tggtgagtgc catgtgctgc
     2101 tggatgtcag actgtaattc acgtgcgccg gctcgccagt cgtctcttgt atagccgatg
     2161 cggcgagcaa ggctgacgaa ctcttctgaa tccggaggag ggagtacaag gtctttggtg
     2221 tttcccctga ccatgcgtaa tccgtcgact aacatccgga agaagatata ggcttttcga
     2281 agaccggctc catcggtgcg cgcgatcaac cccgcattca ccaaggcggc gagtgcctgc
     2341 agggtattgg gcgtatgtag acctgggtgc ttatggccat gcatgacttg gagatattgc
     2401 gcggtatatt cgatatcgat gagcccacct gcgctatact tcacgttgac cttgcccgga
     2461 gcgactagtt gtttgagctg ttgtctcctg agctccaatg ctgtgggaat gtcccagggg
     2521 gcgtctccgt agacgaaaga gtcccggtgt gtttccagtt tacggcccag ggtggagttg
     2581 ccggcgatat gtcgtaactt gatcagggct tgacgctcga acggcgcggc aagtcccgca
     2641 ggactgtagt agcggcagat ttcctcgaac gcattggcga gcgcaccttt tcctccgtgc
     2701 ggtcgaagcc ggacgtcaat gcgaaagatg ccttcctgct tggcttcgat ccatcccagg
     2761 agttcttgtg ccagccgttc gaagtattcg ctattctcaa tttcattctt gccattggtg
     2821 ttcccggatc caccatagac
//
LOCUS       DATMXI010000247         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_140857, whole genome shotgun sequence.
ACCESSION   DATMXI010000247 DATMXI010000000
VERSION     DATMXI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140857"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2569)
                     /locus_tag="VN444_04715"
     CDS             complement(<1..2569)
                     /locus_tag="VN444_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWR21147.1"
                     /translation="MVKRLIDLVFSLVALVLLLPVFLFVAIAIKLDSPGLVFYAQERA
                     GRRGKTFRVLKFRSMVVNAERLGPLVTGNADPRVTRVGRLLRLLKIDELPQFINVLKG
                     DMSFVGPRPEIPIIVEQYTEEQRKVLEAKPGITGPTQLAWVGESDMFPPGVNVSEHYT
                     KQFMQEKLKSDLEYVRTRSLAGDLWYVIRTPMAIGGRILTTLNVPPHIQQYARLTLDC
                     LAVVAANLSAFRVRFDWALPEQEVQHLLYGLPIVCAAYLVSFVTLRTHRSIWKFAGVE
                     DLWQIIKASALGGSLHGLAVVLLGWHGYPRSVLILTAPLTVLLTGGMRLLVRSSAQRQ
                     EAMPSIANGRKVVIIGAGETGELIAREILGNPSLGYGLIGFVDDDPKKNGATIHGRPV
                     LGTIDRLPDIAKTYAIQEAIIAISTATSNELRCISSICARTGLEFRTLPSFTQLVRGD
                     GKLRYLPTVNVDELLGRESVAINREAIAECFRGKRVMVTGAGGSIGSELCRQLIDCGV
                     ASLIMVERAENALHDISLEIRQRSSEADVTAALADVKHIPRMAEIFERTRPQVVFHAA
                     AYKHVPILEDHPGEAVLNNIIGTKRLAEVAQKFDVNTFVFISTDKAVRPKSLMGATKK
                     VSEMYITALNRVLRQSRGVSGTPRCFVVRFGNVLGSNGSVVPLFQKQIENGEPITITD
                     SQMTRFFMTIAEAVGLVLQSSTMDREEDVFILNMGEPIKIDDLVNDLALTSGLNPSRI
                     TKRYIGLRPGEKLHESLWDDEEVLTSEYDRIFAVRQPQVSFGDLEERLSRLETLAMIG
                     DVPALLRQIHDIVPTYVPSLKEPAFSIPEIGEKYRVLVIDDDEAICEMLKAVVNGTYE
                     "
     regulatory      complement(2591..2671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          630 a          804 c          720 g          654 t
ORIGIN      
        1 cctcatacgt gccgttcacg accgctttca gcatctcaca gatagcctca tcgtcatcga
       61 tgacgagaac gcgatacttt tcaccaatct caggaatcga gaatgcaggc tccttaaggg
      121 aaggcacata cgttggaaca atgtcatgta tctgtcgcaa aagggcgggc acgtcgccga
      181 tcatcgccag ggtttcgagg cggctcaggc gctcttccag atccccgaag gagacctgcg
      241 gctgccgtac agcgaagatg cggtcatatt ccgaagtcag gacttcttcg tcatcccaca
      301 agctctcatg caacttctcc cctgggcgca atcctatgta tcgctttgtt atgcgcgatg
      361 ggttcaggcc agaggtgagg gcgaggtcat tcaccagatc gtcgatcttg atcggctctc
      421 ccatgttcaa gataaacacg tcctcttcgc ggtccatcgt actgctctgc aacaccagtc
      481 cgaccgcttc ggctatcgtc atgaagaagc gggtcatctg ggagtccgtg atggtgatgg
      541 gctctccgtt ctcgatctgc ttctgaaaga ggggtacaac gctcccattg ctaccgagca
      601 cattaccgaa tcgcacaaca aagcaacgag gcgtcccgct taccccgcgc gactgtcgaa
      661 ggactcgatt aagggccgtg atgtacatct cgcttacctt tttcgtcgcc cccatcaagc
      721 tcttcggcct cacggcctta tcggtcgaaa tgaatacgaa ggtgttaacg tcaaactttt
      781 gcgcaacttc agccaagcgt ttcgttccga tgatattatt aaggaccgcc tctcccggat
      841 gatcctccag gatcggcacg tgtttatagg ctgcggcgtg gaacacaacc tgcggccttg
      901 tgcgttcgaa tatctccgcc atgcgtggaa tatgcttcac gtccgcaagg gctgccgtca
      961 cgtcagcctc ggaagaacgt tgtcggatct ccagtgagat atcgtgcagg gcattctcgg
     1021 cgcgttcgac catgatgagc gatgcaacgc cacaatcgat cagttgccga cacagttccg
     1081 aaccgatgga tccccccgct ccggtcacca tgacccgctt tccgcggaaa cactcggcaa
     1141 tcgcctctcg gttgatggcc accgattctc gcccaagcaa ttcatcaacg ttcaccgttg
     1201 gaagatagcg cagctttcca tctccccgca cgagctgggt aaaagacggg agggtcctga
     1261 attccaaacc ggttctggcg cagatactgc tgatgcagcg tagctcgtta ctcgttgcgg
     1321 tcgaaatcgc gatgatcgct tcctgaatcg catacgtctt cgcgatgtca ggaagcctgt
     1381 cgattgtgcc gagcacaggc cggccatgaa tggtcgcccc attctttttg gggtcgtcgt
     1441 cgacgaatcc gatgagtccg taacccaacg acggattgcc aaggatttcc cttgcgatca
     1501 attcgccggt ttcccctgcc ccgataatga caaccttgcg gccatttgct atagatggca
     1561 tagcttcctg tctctgggcg cttgaccgca caaggagtcg catccctcct gtaagcagga
     1621 ctgtcaaggg ggcggtcaat atgaggacag atctcggata gccatgccat cccagaagga
     1681 ctacggcgag accgtgcaga cttcctccta acgctgatgc tttgatgatc tgccacaggt
     1741 cctccactcc tgcgaacttc cagatactcc tatgggtccg gagtgttacg aaggatacaa
     1801 gataggccgc gcagacaatg ggaagaccgt acaggagatg ctgcacctcc tgctcaggaa
     1861 gtgcccaatc aaatctgact ctgaaggccg ataggtttgc cgcaacaacg gcaagacagt
     1921 ccagcgtcag cctcgcatat tgttgtatgt gggggggaac gttcagtgtt gtgaggatcc
     1981 ttcccccgat cgccatgggt gtgcgaatga cgtaccagag gtcaccggca agcgagcgtg
     2041 tccggacgta ctccaggtcg ctcttgagtt tctcctgcat gaactgcttg gtatagtgtt
     2101 cagagacgtt aactccggga ggaaacatat ccgattcacc tacccaggca agttgcgttg
     2161 gaccagtgat tcccggttta gcttccaaga ccttacgttg ttcttcggtg tattgttcaa
     2221 ctattatagg aatctctgga cgaggtccga caaagctcat gtcgcctttc aggacattga
     2281 tgaactgagg caactcgtcg atcttcagca gcctgagcaa tctacccacc cgtgtgacac
     2341 gaggatcggc attccctgtc acgagaggcc caagtcgctc tgcatttacg accatggatc
     2401 tgaatttcag gaccctgaag gtcttcccgc gcctcccggc acgctcctga gcatagaaga
     2461 ctagacctgg ggaatcgagc ttaatggcga tggccacgaa gagaaagacg ggcaacagca
     2521 gcacgagcgc taccagggag aatacgagat ctatcaatcg cttgaccatc acctatctcc
     2581 agctcaactc ttcggttgtc cggctatccc aacccatttg ggacccgtgg ctttgcgtcc
     2641 cagccttacg actggtttgc ctttatcgga aaggaccctt ctacaccact gatatgatgc
     2701 gatattcgtg ccagcaagta atcgcaggta catttggcga actcattttt gtgcccagcg
     2761 ttttcaatga gttagaaaat aactgcagag agctgcagac cttctgaa
//
LOCUS       DATMXI010000248         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_200552, whole genome shotgun sequence.
ACCESSION   DATMXI010000248 DATMXI010000000
VERSION     DATMXI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200552"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VN444_04720"
     CDS             complement(<1..444)
                     /locus_tag="VN444_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21148.1"
                     /translation="MQIHEISSRPARLLLVDDNAQNIELLTVLMEAEGHEVISAADGL
                     EALAHVAATPPDLILLDIMMPKLDGYAVCRRLKEETSTRLVPVVLLTALGEEAARIRG
                     IEAGADDFITKPFSRTELKARIRSLLRLKTFTDELEHAETMLLALG"
     gene            complement(610..3537)
                     /locus_tag="VN444_04725"
     CDS             complement(610..3537)
                     /locus_tag="VN444_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR21149.1"
                     /translation="MDREATHRSTSLLQRPRLRVSVRTRLLLLILLAIGPALGLALYA
                     GLQHRRMAAVQAQQEALRLARIAVGDHERLIHGAQQLLIVLSSLPVVRAHDARACDAY
                     LAALLKQYPRYANLGMVTPDGDVVCSALPLPGPINLADRAYVRRAVETRDFAVGDYQI
                     GRITGKATIDFGYPVVSDTGEIETVVFAALDLAWFSRLVTDAQLPPDSVLAVIDRNGT
                     ILARYPNPEKWVGQSASGMPIVKTVLARGEGVLEAPGLDGISRLYGFTPCVSAPHGPG
                     MYVWIGIPGSVAYAEANRILARSLIELGLAGALTIVAAWVFGDLFILRGVRALVHGTE
                     RLKAGDMASRVQVTGRDELSALATSFNDMADRLQAAYSQLTAHVKELTHRERELSLLR
                     EIDQAMLAETPLQNLLSKAVEAFTRLADSQTCVLVTADPESGELKPVANWGPDPEAIR
                     RYFATLRPRVGEEGGCLGRAIATREAVTSPDVACDPSMERYRDAVLTLGIRAVLAVPL
                     LTDQQVLGAAGIGYPTVRRFSETEVRSMEGFANQLAVALEQARLREEAKDHRRLEEAL
                     HASRQLFQAVVQSATDAVIVADGSSRIVACNAGAQRLFGYTEAELLGMSVMTLVPERY
                     REAHRQGLERLEATGEAPVIGRTIEVDALRKDGSDVPVELSLAGWKTGDRVFYSAIVH
                     DITERKQYTTHLEQTVQEKTRTLRATNQRLEAASRHKSEFLANMSHEFRTPLNSIIGF
                     SDILRDPAFGPLTEKQARFAQNILSSGQHLLSLINDLLDLSKVEAGKIDLRPEPFECQ
                     EAIRAAMAEIQPQADLKQLEVTPQTDEAPSTIIADPVRFKQILLNLLSNAVKFTPEGG
                     RITVTARRAARQEAESRRQETEGRTWNLSTLHPGDFVEIAVADTGIGMKPDDLPRLFQ
                     KFTRLDAAVAQRTKGTGLGLALTKRLVELHGGEIAAASDGEGQGSTFTVRLPMTPADH
                     YAL"
BASE COUNT          659 a         1136 c         1083 g          696 t
ORIGIN      
        1 gcccagtgcc agcagcatgg tttcggcgtg ctccaactcg tcggtaaagg tcttgagccg
       61 gagcagtgaa cggatgcgag cctttagctc cgtccgactg aacggcttcg tgatgaagtc
      121 atcagccccc gcctcaattc ctcggattcg ggccgcttcc tcgccgagag ccgtaagcaa
      181 cacgacaggc accaggcggg tggacgtctc ctctttcagg cgacggcaga ccgcgtaacc
      241 atccagcttc ggcatcatga tatccagtag aatcaggtcc ggaggggtgg cagccacatg
      301 agccagggcc tccagaccgt cagcggccga gatcacctca tgtccctcgg cctccatcag
      361 gaccgtcagc agctcgatgt tctgtgcatt gtcgtcgaca agaagcagtc gtgccggcct
      421 tgaagagatc tcgtgaattt gcatggcggc atctcactcc tgcaagtgtt cagtgctatt
      481 ttgtacttta taaggatcat acctgacggc cattaaaaga tcaagcgccg atccctctgc
      541 gcgtcgtctt ctccgagcgc aggtgtcgtg taggctgtac cgtcttgaaa ccgtcggcgt
      601 gaacgtctat tagagagcgt agtggtcggc aggggtcatg ggaagccgga ccgtgaaggt
      661 gctgccctgt ccttcaccgt ccgaggcggc agcgatttcc cccccgtgca gctcgacgag
      721 tcgcttcgtc agggcgaggc caagtccggt tcccttcgtc cgctgggcga ccgccgcatc
      781 aaggcgggtg aacttctgga agagtcttgg cagatcgtcc ggcttcatcc cgatccccgt
      841 atcggcgacg gcgatttcga caaaatcgcc cgggtgcaga gtgctgagat tccaagtccg
      901 gccttctgtc tcctgcctcc tactttctgc ttcctggcgc gcggcgcggc gcgcagtgac
      961 cgtgatccgt ccgccttccg gcgtaaactt gacggcattg gaaaggaggt tcaacaggat
     1021 ctgcttgaaa cggacgggat cggcgatgat cgtggacggc gcctcatccg tctgcggcgt
     1081 cacctcaagc tgtttgaggt cggcctgggg ctggatttcc gccatggcgg cgcgtatggc
     1141 ctcttggcac tcgaagggtt cgggacgcaa gtcgatcttg ccggcctcta cctttgacag
     1201 gtccagcagg tcattgatca gcgacaacag gtgctgcccg ctcgacagga tattctgtgc
     1261 gaaccgcgct tgtttctccg tcaaggggcc gaaggccggg tctctgagga tatcggaaaa
     1321 gccgatgatc gagttgagcg gcgtccggaa ttcatgcgac atgttggcca gaaactccga
     1381 cttgtgtcgg gacgcggctt cgagtcgttg attggtcgct cgcagcgtcc gcgttttctc
     1441 ctggaccgtc tgttccaggt gtgtcgtata ctgtttgcgc tcagtgatat cgtgcacgat
     1501 agcgctgtag aaaacccggt ctcctgtctt ccaaccggcg agcgacaact ccaccggtac
     1561 gtcgctgccg tccttccgca gcgcgtcaac ctcgatcgtt ctaccgataa caggggcctc
     1621 gcccgttgcc tccaaccgct ccagtccctg tcggtgtgcc tcccggtatc gctctggcac
     1681 cagggtcatg accgacatac ccagcagctc tgcttcggta tagccaaaga ggcgctgggc
     1741 gcctgcgttg catgccacaa tgcggctgct gccgtcggcg acaatgaccg cgtcggtggc
     1801 cgactgcact accgcctgga acaactgccg actcgcatgg agcgcctcct ccagccgccg
     1861 gtgatccttc gcttcctccc gaaggcgggc ctgctccaaa gccacggcga gttggttcgc
     1921 gaagccctcc atggaccgga cctccgtctc cgagaagcgc cgtactgtcg gatagccgat
     1981 ccctgcggcc cccagcacct gctgatccgt aagcaacggc accgcgagaa ccgcgcggat
     2041 accgagggtc aacacagcat cgcggtaccg ctccatcgaa ggatcgcagg caacgtccgg
     2101 ggacgtgacg gcctcccgcg tggcgatcgc cctcccaaga cagccgccct cctctcctac
     2161 tcgcggccga agggtcgcga agtaccgccg gatcgcctcc gggtccggcc cccagttcgc
     2221 aaccggcttc aactctccgc tctccgggtc ggcagtgacc aagacgcagg tctgcgagtc
     2281 tgcgagccga gtgaacgcct caacggcctt cgagagaagg ttctggagag gtgtctcggc
     2341 cagcatcgcc tggtcgatct cccggaggag gctgagctcc cgctcgcgat gcgtaagctc
     2401 cttgacatgg gcggtgagtt ggctgtaggc agcctggagg cgatccgcca tgtcattaaa
     2461 gctggtggcc agggcgctaa gctcgtcgcg tccggtcacc tgcactcggc tagccatatc
     2521 tccagccttc agtcgctcgg tcccatggac cagagccctc accccgcgca ggatgaacag
     2581 gtcaccgaat acccacgccg ccacaatggt cagcgcacca gcgaggccga gttctatgag
     2641 gctacgggcc agtatccggt tagcctcagc atacgctact gaccctggaa tgccgatcca
     2701 tacgtacatg cctggtccat gtggtgcgct gacacacggg gtgaacccgt agaggcggga
     2761 gatgccgtcc aggccaggag cctcaagtac gccttcgccc ctagcgagaa cggttttgac
     2821 aatgggcatt cccgatgcgg actgcccaac ccatttctcc ggattgggat agcgggccaa
     2881 gattgtgcca ttgcggtcga ttacggccaa taccgaatcc ggtggcaact gggcatcagt
     2941 gactagccgg ctgaaccagg ccaaatccag cgcagcgaag actactgtct cgatttcccc
     3001 ggtatcactg acgacgggat agccgaaatc gatcgtggct ttgccggtga tacggccgat
     3061 ctggtagtcg ccgacggcga agtcgcgggt ctccacggcg cgccgaacat acgcccgatc
     3121 ggcgaggttg atggggccgg gcagcggcag cgcgctgcac acgacatcgc cgtctggcgt
     3181 caccataccc aggttggcgt atcgaggata ctgcttcagg agagcggcaa ggtacgcgtc
     3241 acacgcgcga gcatcatgcg cgcggactac gggcagcgac gacagcacga tgagaagttg
     3301 ctgcgccccg tgtatcagtc gttcgtggtc gccgacggcg atcctcgcca gccgcagcgc
     3361 ctcttgttgg gcctgaacgg cggccatccg gcgatgttgc aggcccgcgt agagcgcaag
     3421 tcccaatgcc ggcccgatcg ctaagaggat aagcagcaac aggcgggtgc gaacacttac
     3481 ccgcagccgc gggcgttgca acagagaagt cgatcgatgc gtggcctctc gatccatccg
     3541 aaatcacttc gttcaagccg ggcgagtcgt cgtt
//
LOCUS       DATMXI010000249         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_288614, whole genome shotgun sequence.
ACCESSION   DATMXI010000249 DATMXI010000000
VERSION     DATMXI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288614"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VN444_04730"
     CDS             complement(<1..1015)
                     /locus_tag="VN444_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR21150.1"
                     /translation="MEGYSTKVLIVDDDASARKILQSRLRAMNVQVLVASGGSEALEQ
                     IRQETPAVVLLDLQMPKMSGIDVLRSLKREGLEATVIVVTAHATIETAVEAMKEGAYD
                     FITKPVDVKHLEIVLGKAFERESLRAQSRFLQTEMEGRLVQVVTENPAINNLLQLARR
                     AASSSSTILILGESGTGKEVLARSIHRWSPRANYPFTVVNCVAIPDQLLESELFGHER
                     GAFTGAHQLKRGKFEIADRGTVFLDEIVEVPASIQTKLLRVLQDHEFERVGGTRTIRT
                     DIRVIAATNGDLERAMREGGFREDLYYRLNVVSLKLPPLRERKEDIPVLIEYFLRKYA
                     GELK"
     gene            complement(1097..1468)
                     /locus_tag="VN444_04735"
     CDS             complement(1097..1468)
                     /locus_tag="VN444_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21151.1"
                     /translation="MSGKLILHVEDNEYNRKIIRDLLSKNSYEIVEAHNGEAALDALA
                     RQHPDLILMDVQLPKLSGLDATRRIRADPLLAQIPVIAITSFALSGDDRLAIEAGCNA
                     YIAKPFRPRDLLEMIRRFIGP"
     gene            complement(1465..>2059)
                     /locus_tag="VN444_04740"
     CDS             complement(1465..>2059)
                     /locus_tag="VN444_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21152.1"
                     /translation="DVLDLSKIEAGHMQLNLGEYSVQSFIDSVISATRSLAAEKRLEL
                     VGRVEEGLPTAVGDSKRIIQILMNLVGNAIKFTPAGGSVIITARCVRAKERAVPGARC
                     EEEEAKPWNSGDQIEITVADTGIGIPAEELKSIFGEFRQVDSSITREYGGSGLGLSIA
                     KRLVEMHGGSIWAESQVGKGSTFYFRIPLRTQWGGSP"
BASE COUNT          406 a          640 c          534 g          479 t
ORIGIN      
        1 tcttcaactc gcccgcatac tttcgcagga agtactcgat cagaaccggg atatcctctt
       61 ttcgttctct cagcggcggt agcttcaggc tgacgacgtt cagtcgatag tagagatcct
      121 cgcgaaagcc gccctcccgc atagctcgtt cgaggtcgcc attggtagcg gcgataacgc
      181 gaatgtcggt cctgatcgta cgcgttcccc caacccgttc aaactcatgg tcttgcagga
      241 cccgaagcag tttcgtttgg atgcttgccg ggacctccac gatttcgtcg agaaacaccg
      301 tgccgcgatc cgcaatctcg aacttccccc ttttgagctg atgggcgcct gtgaaggctc
      361 ctctttcgtg gccgaagagc tcactttcca gtaactggtc cgggatggcc acgcaattga
      421 cgacagtgaa tggatagttg gcccgaggac tccaccggtg aatgcttctg gccaggacct
      481 ctttccctgt cccgctctcg ccaaggatca ggatggttga actgctgctg gcggcgcgac
      541 gggcaagctg cagcaggtta tttatagctg gattttccgt gacgacctgg acgaggcgac
      601 cttccatttc tgtctgcaaa aagcgacttt gtgctcgcag tgactcccgc tcgaatgcct
      661 tacccaggac gatctccaga tgctttacat cgaccggctt ggtgataaag tcgtaggcgc
      721 cctctttcat ggcctctacc gcggtctcga tggttgcgtg cgcggtgaca acgatcaccg
      781 tcgcttccag cccttcgcgt ttcaatgatc tgagaacgtc gatccctgac atcttcggca
      841 tctgcagatc aagcaggaca actgcgggcg tctcctgccg gatttgttcc agggcctcag
      901 agccgccgga cgcgacaagg acctgcacgt tcatcgcgcg caaccggctc tggagaatct
      961 tcctggccga tgcatcatcg tcgacgatca gaacttttgt gctatatcct tccatctctc
     1021 ctccagcaaa gagtccctcc tgcactcacc agtaacccct gcctgccggt ttcgacagtc
     1081 tcttcccaaa cagcgctcat ggacctatga agcgtcgaat catctccaag aggtcccgtg
     1141 gccggaaggg ctttgcgata taggcgttac accccgcctc gatggccagc cggtcatcgc
     1201 cgctcagggc aaacgaggtg atggcgatga ccgggatctg ggcaagcaag ggatccgctc
     1261 tgatcctgcg agtcgcatcg aggccggata gcttgggcaa ctgaacatcc attaggatga
     1321 gatcagggtg ctgtctagcc agcgcatcga gcgcggcctc cccattgtga gcttccacga
     1381 tctcgtaact gtttttggag agaaggtccc tgatgatctt ccgattgtat tcgttgtcct
     1441 caacatgtag gatcagcttg ccgctcacgg gctgccaccc cactgggtcc ggagcggaat
     1501 cctgaagtaa aaggtcgacc cctttccgac ctggctttcc gcccagatag accccccatg
     1561 catctcgacc aaccgcttgg cgatgctgag ccccaaaccg cttccgccgt attctctggt
     1621 gatggagctg tcgacctggc ggaattcgcc gaagatactc ttcagctctt ctgcgggaat
     1681 tccgattcca gtatctgcca cggtgatctc aatctggtca ccggaattcc agggtttagc
     1741 ctcttcctcc tcacatctcg cccctggtac cgcgcgctcc ttagcacgta cacaacgggc
     1801 ggtgatgatg accgaccccc ccgctggggt gaacttgatg gcattgccca ccagattcat
     1861 caggatctgg ataatgcgct tgctatcgcc caccgccgtc ggcagaccct cttcaacccg
     1921 gccgaccaac tccagccgct tttctgctgc gagggatcgc gttgctgaga tcacagagtc
     1981 gataaatgac tgaacagagt attcacccag attcaactgc atgtgtccgg cttcgatttt
     2041 cgagagatcc aacacatcg
//
LOCUS       DATMXI010000250         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_112146, whole genome shotgun sequence.
ACCESSION   DATMXI010000250 DATMXI010000000
VERSION     DATMXI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112146"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="VN444_04745"
     CDS             complement(<1..2258)
                     /locus_tag="VN444_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21153.1"
                     /translation="MGDFCLELHSHKANKRQVVAELKRCLEERPVPQRSLSALEFEKL
                     KRLKDTLNNYVIALHRLRSPLERSVYEVLSLLARLDATPRVAMELSEPNKLTPGRLQE
                     VEEMVRRLATVWQVIEEGEDFSWKGFTGDQFTIDLRSAMISLLDQLMSSIDRLSLVSA
                     QYASEIGLDIPLRLDEVEWLISVSNLLISSPGPDSSWLTHPHLDRLVAEARHYQELCS
                     SYRTMRDALKERHTPTFFDLPRGLAVELANARTQTSHLLAPGDFDGALLLKQRNELRQ
                     FVMNTQRTVTEWGRDATEIARGLGLPSNDLTMDKARQLAQLAILCHSESRPAASWLDP
                     IRAQHLRDILPRVRADYEKYNETKRQLLEFYDESFLELELDRLIEQYQGPYRGIFRWF
                     IPRFHRDRRTIARTTQTGVVPESILEDLLTARKLRRLATQLAAERDHVRDLLGGYDQG
                     YDTKFDLVERALAVAAEAIALAGTTPVPNELIRNISLGTTPSPEIRISGTRLRDSLED
                     WKQTAHAFSSLLFGCKAAIDPNRVSPSDRLPGSDYSLLQTPVSEIERWAHEIRTPLDS
                     LCRLTEQVLNTCIGEPPIDYRAILTDLEKLKKLVEVESGLAAESERLRSQFGARYNGV
                     DADWANVLAALDWTGQVQKLFGSRTIPDRLCLIASQGPMAAPSNGELTQACLLTKVLL
                     QSLEARFDLPTPPEERPGFLQAAEDKLKARSERSGLTHRAVAFRELTLERLHDRLGEL
                     RSRVDDLQTWID"
BASE COUNT          511 a          626 c          622 g          523 t
ORIGIN      
        1 aagtcaatcc aggtctggag gtcgtccact cgggagcgaa gctctcccag ccgatcatgt
       61 aggcgttcaa gcgtcaattc acggaacgct actgcccgat gagtgagacc ggaccgttcg
      121 gatcgagctt taagtttgtc ctccgcagct tgcagaaatc ccggcctttc ctctggcggc
      181 gttgggagat caaatcgagc ctcaagggat tggaggagaa ccttagttag cagacaagct
      241 tgggttagct caccatttga gggggccgcc atcggtccct gggaggcgat caagcagagc
      301 cgatcgggga ttgtgcgtga gccgaacagc ttctgaacct gccctgtcca gtctagcgct
      361 gctaggacgt tggcccaatc tgcatcaacg ccgttgtacc gggcgccgaa ttgtgatcga
      421 agccgttccg actccgctgc aaggccggat tctacttcaa ccaacttctt caacttctcc
      481 aggtcagtaa gaatcgctcg atagtcaatc gggggttcgc cgatgcaggt attgagtacc
      541 tgctctgtaa gacgacagag ggagtcaagg ggtgttcgta tctcatgcgc ccagcgctca
      601 atctccgata ccggggtctg caagagagaa taatcagaac caggcagccg atcggatggg
      661 ctgacgcggt taggatcgat agcagcctta catccgaaaa ggagtgaaga gaaggcgtgc
      721 gccgtttgct tccaatcttc caaagagtcc ctgagacggg ttccactaat gcggatttcg
      781 ggagaaggcg ttgtccccag cgagatattt cgaataagtt catttgggac cggggtagta
      841 ccagccagcg caatggcctc ggcagccaca gcaagggcgc gttcaaccaa atcgaatttc
      901 gtgtcgtaac cctggtcata tccccccagc agatcgcgaa catggtcccg ctcagccgcg
      961 agttgcgttg caagacgccg cagcttccgt gccgtaagca aatcctccag gatcgactcc
     1021 ggtaccacgc cagtctgggt cgtacgggct atggttcgcc tgtcccggtg gaatcttgga
     1081 atgaaccatc gaaagattcc tcggtaggga ccttggtatt gctcgatgag ccggtcaagc
     1141 tctaattcaa ggaaactttc gtcgtaaaac tccaggagtt ggcgttttgt ctcgttgtat
     1201 ttctcgtaat ccgccctcac ccgaggcaag atatcgcgta gatgttgcgc acgaattggg
     1261 tccagccaac tcgcggccgg ccggctctcg gaatgacaca ggatggccag ttgcgcgagt
     1321 tgccgtgcct tatccatggt gaggtcgtta ctcggaagac cgagacctct ggcgatctca
     1381 gttgcatcgc gcccccattc cgtaactgtt cgttgggtat tcatgacgaa ctgccggagt
     1441 tcattgcgct gctttaatag cagagcccca tcaaaatcgc ctggggcgag caaatgcgat
     1501 gtctgagtcc gcgcgtttgc aagctcaact gcaagccctc gcggcagatc aaagaacgta
     1561 ggagtatgac gttccttgag agcatctctc attgttctat acgaactaca cagctcttga
     1621 tagtgccgcg cctcagcaac caaacggtct aggtgcggat gagttaacca gctcgaatca
     1681 ggacctgggc tactaataag aaggttgctg acgctgatta accactccac ttcgtcaagg
     1741 cgtaatggaa tatccaggcc gatttctgag gcatactggg ctgacaccaa actgagtcgg
     1801 tcaattgaag acatcaattg gtcgaggagg cttatcattg ccgaacgcag gtcgatggta
     1861 aattgatccc ccgtgaatcc cttccacgag aaatcctccc cttcctcgat gacctgccaa
     1921 accgtcgcta ggcggcgtac catttcctcg acctcctgca accgtcctgg cgtcagtttg
     1981 tttggttcag acaactccat tgcaacacga ggggtggcgt ctaatcttgc caggagactg
     2041 aggacttcgt agacgcttcg ctccaagggg ctccgcagtc gatggagcgc gatcacgtag
     2101 ttatttagcg tatcctttag ccgtttcaac ttctcaaatt cgagcgctga taacgacctc
     2161 tgtgggacag gacgttcctc gaggcaacgt ttgagctcgg ctacgacctg ccgcttgttg
     2221 gctttgtgac tgtggagttc cagacaaaag tcacccaaac catcctcccg aagccgtttg
     2281 ta
//
LOCUS       DATMXI010000251         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_291689, whole genome shotgun sequence.
ACCESSION   DATMXI010000251 DATMXI010000000
VERSION     DATMXI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291689"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..858)
                     /locus_tag="VN444_04750"
     CDS             complement(49..858)
                     /locus_tag="VN444_04750"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HWR21154.1"
                     /translation="MPPVVLCMKEAPTSIPLEAESISPDLFVQRSQAEIEAMPVALGN
                     ETLRLGDFFKVEGERSDEIVLEGCTGRVKWIGSGMTRGRIVIRGDVGMHAGSYMRGGE
                     ILVEGNADDFLGVEMEGGLIRVNGSAGHRAGAAYRGSKYGMQGGTILVDGNVGHEVGA
                     YMRRGLIVIKGNAEDFLGAMMATGTICLFGQAGIRTGGGMQKGTIICMRPVDLLPTFA
                     YDCTYAPVFLRILFKELKQLGVECPAQADGLVRRYHGDLADVGKGEILIAA"
     gene            complement(892..1806)
                     /gene="fhcD"
                     /locus_tag="VN444_04755"
     CDS             complement(892..1806)
                     /gene="fhcD"
                     /locus_tag="VN444_04755"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HWR21155.1"
                     /translation="MEINGVYIDETYAEAFGAYAGRVLITACSKTWAQEAARSMAGFG
                     TSVIGCGCEVAIEAIEPADTPDDRPGVSVLLFAMSKKAVQEQLINRIGQCVMTAPTSA
                     CYNALEAEDRLSIGGKLKFFGDGFQISKRLDSRMTGNGSDPRRFWRIPVMDGEFIVED
                     RFGVKKGVAGGNFLLLGRELPGTLQAAERAIEAMRKVPDVIMPFPGGVVRSGSKVGSR
                     YKFLNASSNTAFCPSLRRAVKSELPDGVGAVLEIVIDGLTPEAVGEAMRVGIRAACGP
                     GVMQITAGNYGGKLGKHHFHLHQLLAEG"
     gene            complement(1834..>2759)
                     /locus_tag="VN444_04760"
     CDS             complement(1834..>2759)
                     /locus_tag="VN444_04760"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HWR21156.1"
                     /translation="TPNNYLTTLDTMQTLEGSRLHVAHAQFHSYGGENWGGFCSKAAE
                     IAEYINTHPNITTDIGQIVFGDATTITADGPWQYRLYKLSKNKWYNQDIEVESGCGIV
                     PYTYRENNFVNSLQWAIGLELFLLIRDPWQVFLSTDHPNGGPFYLYPWIIRLLMDKPF
                     RDEMLKRVHKRVMPETILKALDREYTLYEIAIISRAGPARALGLKHKGHLGVGADADI
                     TIYSKDSDKEQMFEHPVYVIKAGETVLRDGEIVHSPDGKTLFVVPPDVGEMDPTFKTE
                     FENYYTIRYQNFPIDIEDIPNREAIPVGDPR"
BASE COUNT          581 a          887 c          708 g          583 t
ORIGIN      
        1 atctcacctg cccggatatt cagactgagc gacaaggtcg cgtcctcctc aggcggcaat
       61 cagaatttcg cccttgccga catcagccag gtccccatga tagcgccgaa caaggccatc
      121 cgcctgagca gggcactcca cccctagctg tttcagttcc ttaaacagaa ttcggagaaa
      181 gaccggcgca taggtgcagt cataagcgaa ggtcggcagg aggtcaacag gacgcatgca
      241 gatgatcgtc cccttctgca ttccgcctcc tgttctgatg cctgcctggc caaagaggca
      301 gatcgtccca gtcgccatca tggcccccag aaagtcctcg gcgttcccct tgatgacaat
      361 gagacctcgc cgcatatagg cgcccacttc gtgcccgacg tttccgtcga ccaggatagt
      421 cccgccctgc ataccgtact tgctcccccg ataggcggcc ccggcacgat gccctgcact
      481 accattgacc cggatcagcc cgccttccat ttcgacaccg aggaagtcat ccgcattccc
      541 ttcaacgagg atctcgccgc ctcgcatata cgatccggcg tgcatcccga cgtcgccgcg
      601 gatcacaatt cgaccgcgcg tcatccctga gccgatccac ttgactcgtc ccgtgcagcc
      661 ctctagaacg atctcatccg atcgctcccc ctccaccttg aaaaagtcgc caaggcgaag
      721 ggtttcattc cccaacgcga cgggcatggc ctcaatctcc gcctggctgc gctgcacgaa
      781 cagatcgggc gagatcgact cggcctcgag cggaatactc gtcggggcct ctttcatgca
      841 aagcacgact ggtggcatgt taccctcttc tcacaagtga gccatcagct atcagccctc
      901 agccaacagt tgatggaggt gaaagtgatg tttccccaac ttcccgccgt agttaccagc
      961 ggtgatctgc atgacgcctg gaccgcacgc ggcccggatg ccaactcgca ttgcctcccc
     1021 taccgcctcc ggcgtcaggc catcgatgac gatctcgagg accgccccaa ccccgtccgg
     1081 tagttcgctc ttgaccgctc gcctgagcga tggacagaag gctgtgttgc ttgaggcatt
     1141 caggaactta tagcgcgagc cgaccttact accggacctc acgacgccgc cagggaacgg
     1201 catgatgaca tccggcacct ttcgcatcgc ctcgatcgcc cgctccgcag cctgcagggt
     1261 cccaggcagt tcgcgcccca gcagtaagaa gttgccgccg gccactccct ttttcacccc
     1321 gaaacggtct tcgacaatga actcaccgtc catcacagga atccgccaga agcgccgcgg
     1381 atcgctccca tttccggtca tccgtgagtc gagccgctta ctgatctgaa atccgtctcc
     1441 aaaaaacttg agcttgcctc ctatcgacag gcgatcttcc gcctccaggg cgttataaca
     1501 ggccgaggta ggagcggtca tcacacactg accgatccgg ttgatgagct gctcctgaac
     1561 cgcctttttt gacatggcaa acagcaacac actcacgccg gggcggtcat ccggcgtgtc
     1621 cgccggctcg atcgcctcaa tggcaacctc gcaaccacag ccgattaccg aggtcccgaa
     1681 gcccgccata ctccgagccg cctcctgggc ccacgtcttg ctgcacgcgg taatcagcac
     1741 tcgaccggca taagcgccga atgcctctgc gtatgtctca tcgatgtaaa cgccattgat
     1801 ctccatcgct tctccgtcag atcgacaaca atatcatcga ggatctccca caggaatcgc
     1861 ctcccgattg gggatgtctt cgatatcgat tgggaaattc tgataccgaa tcgtgtagta
     1921 gttttcgaat tcagtcttaa aggttgggtc catctcgccg acgtcaggcg ggacaacaaa
     1981 tagggtcttt ccatcgggac tgtgaacgat ctcaccatct cgaaggaccg tctcgccggc
     2041 cttgataaca taaacgggat gctcgaacat ctgttcctta tcgctatcct tggaatagat
     2101 agtaatatcg gcatccgccc cgactcccag gtggccctta tgcttcaatc ccaaggctct
     2161 tgcgggtcca gccctggaga tgatggcgat ctcgtaaagg gtatactcgc ggtcaagggc
     2221 cttcaggatg gtctccggca tcacacgctt atggacgcgc ttcagcatct catcacggaa
     2281 cggtttgtcc atgagcagcc tgatgatcca gggatagagg tagaaggggc cgccgttggg
     2341 atgatcggta gagaggaata cctgccacgg atcacgaatc aggaggaaga gctccagtcc
     2401 aatggcccat tgaagggagt tcacaaagtt attctctcga taggtgtacg ggacgatccc
     2461 gcatcccgac tcgacctcta tgtcctggtt gtaccattta ttcttactga gcttgtatag
     2521 ccggtactgc cacgggccat ccgccgtgat agtggtagca tcgccgaaga caatctggcc
     2581 gatgtccgtc gtgatgttgg gatgggtgtt gatatactcc gcgatctcgg ccgcctttga
     2641 gcaaaaaccg ccccagtttt caccaccgta actatggaac tgggcatgcg ccacgtgaag
     2701 ccgggagccc tcaagcgtct gcatcgtgtc cagggtggtc aggtagttgt tgggtgtgc
//
LOCUS       DATMXI010000252         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_306582, whole genome shotgun sequence.
ACCESSION   DATMXI010000252 DATMXI010000000
VERSION     DATMXI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306582"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VN444_04765"
     CDS             complement(<1..1007)
                     /locus_tag="VN444_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21157.1"
                     /translation="MNQVDQHTLDVLEWPAIQARLAALAGSSVGRELAQSTRPLPTLE
                     EAKKVRSEVEEFRALLSRETSLPFDQLFDTRESVRRSRPEGAVLAAMDFVRVATSLEA
                     AEAIRHAIARSRNLCPQLHALASRLDDHTGLVREIHAAIDAAGEVKDTASRKLNQLRL
                     RLHEIRNLIYSRLQSLLTDPHLQPSIAEPLVTLRNERFVIPVKPNYRTVLKGVVQDRS
                     VSGATIFLEPQEVVELNNHLRLLQQSEEEEIRRVLVALTTSLRSQAEAVLSTILLTAE
                     LDARCAAARFADRLHCAPVSLKDTGPLVLREARHPLLLEQTEAAGTIQAIPIDLRLGD
                     S"
     gene            complement(1165..1617)
                     /locus_tag="VN444_04770"
     CDS             complement(1165..1617)
                     /locus_tag="VN444_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWR21158.1"
                     /translation="MSVSKARLAEDLKAALKGGDRLRTSVLRLLCSLIKNKEIEKRGE
                     LDDSEIIQAVIASCKLRKEAIEQYTKGGRDDLAAKEEAELKLLEAYLPPPLSSEELRK
                     KIEEALAASQASSLKDMGKAMALLMPEIAGRADGKVVSQMVKDALSRR"
     gene            complement(1621..1818)
                     /gene="rpsU"
                     /locus_tag="VN444_04775"
     CDS             complement(1621..1818)
                     /gene="rpsU"
                     /locus_tag="VN444_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWR21159.1"
                     /translation="MASVTVKENEPFEVALRRFKKLCERTGVLSELRKREHYEKPSVR
                     RKKKSIAARKKTLKRVSFTVE"
     gene            complement(2027..2215)
                     /locus_tag="VN444_04780"
     CDS             complement(2027..2215)
                     /locus_tag="VN444_04780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWR21160.1"
                     /translation="MATAKTAAPNSAGSQLYITMAPQPALDSGYTVFGQVVEGMDVFM
                     KIKRGDIMTKVTVVEATP"
     gene            complement(2270..>2445)
                     /locus_tag="VN444_04785"
     CDS             complement(2270..>2445)
                     /locus_tag="VN444_04785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWR21161.1"
                     /translation="QFFLTVAPTPFLDGKHSVFGEIVEGLDVAIAISNVPRDSNDRPR
                     EDVKITKVTIVRE"
BASE COUNT          471 a          733 c          686 g          555 t
ORIGIN      
        1 aagctatcgc ccagacgcag gtcgatcggg atggcctgga tcgtcccggc cgcttccgtc
       61 tgttccagta agagtgggtg ccgggcttcc cgcagcacca ggggacccgt gtccttcaag
      121 gagacagggg cacagtgaag ccggtcagca aatcgcgccg cggcacatcg ggcgtccagc
      181 tcagccgtga gaagtatcgt cgagagaact gcctccgcct gagaccggag agaggtggtg
      241 agcgcgacca gcacccttcg aatctcctcc tcttcggatt gctgcaacaa ccgtagatgg
      301 ttattgagtt ccacaacctc ctggggttcc aggaagatgg tggcgccgct gacggatcga
      361 tcctgaacga cacccttcag gacggtccgg tagttgggct tgacagggat cacgaaccgc
      421 tcgtttcgga gcgttacgag cggttcggcg atagagggct gaagatgagg atccgtcagt
      481 agggactgta gacgagaata gatcaggtta cggatctcat ggagtcgaag tcgaagttgg
      541 ttgagcttcc ggctggcggt gtccttgacc tctccggctg catcgatggc cgcgtgaatc
      601 tcccttacca ggccagtatg atcatcgagt ctggaggcga gagcatgaag ctgcgggcac
      661 aggttcctgg agcgggcgat cgcgtgacgg atcgcctccg ccgcttccag cgatgtggcg
      721 accctgacga aatccattgc cgccaggacg gccccttccg gtcgagaccg ccgaaccgac
      781 tccctggtgt caaacagttg gtcaaatgga agtgaggttt ccctggacag cagcgcccgg
      841 aactcctcaa cctcgctccg gactttcttc gcttcctcca gcgtggggag tgggcgggta
      901 gactgtgcca actcccttcc cacggacgat ccggccaggg cggccaatct cgcctggatg
      961 gcaggccact ccaggacatc gagggtgtgc tgatcgacct gattcatctg acgcgagccg
     1021 ctctagatgg cttcagccgc agcgagcgca cattccgagt gcgtggatgc accataaata
     1081 ctacagggga ctcgatttcg agccccctga gcgcggacca ccccagcacg acggtacact
     1141 cacgatgaga caattgcgca gttatcaccg tcgcgacaat gcatctttta ccatctggct
     1201 aactaccttc ccatcagccc ggcccgcgat ctccggcatc aggagggcca tggccttccc
     1261 catatccttg agggaactcg cctgcgacgc ggcaagcgcc tcctctatct tttttcgaag
     1321 ctcttcagac gagagcggtg gagggagata ggcctcgagg agcttgagct cggcctcttc
     1381 cttcgcggcc agatcgtctc gtcccccctt cgtatactgt tcgatggcct cttttcggag
     1441 cttgcaggag gcaatcactg cctggatgat ttcagagtcg tctaactccc cccgcttttc
     1501 tatttccttg ttcttgatga gcgagcagag aagccgaagc actgacgtcc tcaaccgatc
     1561 ccctcccttg agggccgctt tgaggtcttc ggctaatcgt gcctttgaaa cgctcacgtt
     1621 ctactctaca gtgaagctga cgcgcttcaa ggtcttcttc ctggcggcta tcgacttttt
     1681 cttgcggcgg acactgggct tctcgtaatg ctcgcgcttg cggagctctg acaggactcc
     1741 agtcctctcg cacagtttct tgaaacggcg gagagcgacc tcgaacggct cgttctcctt
     1801 gaccgtcaca ctcgccatcc ccttcacacc cccttagtaa tagcgatgag ataagctaca
     1861 gtttgatacg atgctttacg gaatccaggc gctgctgcgt cgagtttcga cgagtgctat
     1921 catggcacct ttcgtacata aatgtcaata tgtttgccag cgccgggtct ctccaaactc
     1981 ggggatccag atgcgctcag gctcgattcc tcgccgcctg atcggctcag ggggtggcct
     2041 cgacaacggt aaccttcgtc atgatgtccc ctcgcttgat cttcatgaag acatccatcc
     2101 cctccacgac ctggccgaac actgtgtacc cgctgtccag cgcgggttgt ggcgccatgg
     2161 tgatgtagag ctgactcccc gcgctattgg gcgcagcggt ctttgccgtg gccatcgttc
     2221 cggtcaggtg tttgcgcgta ttgacctcat ccttatcctt taactttcgc tattctcgaa
     2281 cgatagtcac tttggttatt ttcacatctt cgcgcggtcg atcattgctg tcgcggggaa
     2341 cgttgctgat agcgatggcc acatcaagac cttctacgat ttcaccgaag actgagtgct
     2401 tgccgtcaag aaacggagtg ggcgcaacgg tgagaaaaaa ttgac
//
LOCUS       DATMXI010000253         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_286433, whole genome shotgun sequence.
ACCESSION   DATMXI010000253 DATMXI010000000
VERSION     DATMXI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286433"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="VN444_04790"
     CDS             complement(<1..2096)
                     /locus_tag="VN444_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21162.1"
                     /translation="MKFSLSEQSLRVIHCGKPFDQADVEAICSVGETTKELTDIGRFG
                     IGFKSVYAFTDRPQIHSGQEDFAIEDYIRPYAISPSDREVEETVILIPRKPSDEGFDF
                     EIEAGLNRLGPSTLLFLREIEEIEWSVDGGPSGLYLRSQPERLGDGVRRITVIGQAEG
                     HPDVEESWLVFSKPVCTPEGNQAGYVEIAFSLAKDDKSGQQRLHPVSRSRLVVFFPTV
                     VDTYLGFLVQGPYRTTPSRDNVPPHDAWNRYCVNETATVLLEALHWLRDKDLLDVGVL
                     RCLPLDRSKFGERTMFAPIFEATKEALANEPLLPRYGGGHVAARQATMARTQELRELF
                     SPAQLAALLGADGELGWLSGDISPDRSPDLRDYLIDELDIEEVRPETILPKLNRMFLE
                     AQPEEWVRRLYEFLGGQKALLWRAKELPLIRLTDGKHVRAHANGQPQAFLPGAIETGF
                     PTVHPAVCNTEESRAFLRTLGLTEPDPVDDVVRNLLPKYCVDGTNVGEEEYHVDIRRI
                     LAAFKTDSDSQRKKLVAALRQTPFVIAVDAGDCSRRMSRPGETYLATERLTELFSGVR
                     GVLLVDNSRLSLRGDEVRKLLEASGGNPCLRPIPVTPNFTWEELRYMRIAVGCESMSS
                     WEPLEDSTLYGLDEVLRHLVTLDPQNRKTRAAHLWAALGDLEDRRGTGVFSGTYQWTY
                     YHRRTAPFDAAFVRLL"
BASE COUNT          503 a          734 c          662 g          507 t
ORIGIN      
        1 ttgagtaacc gcacgaaggc agcatcgaac ggggcagttc gtcgatggta gtaggtccac
       61 tggtaggtgc cggagaacac acctgttccc cggcggtcct ccaagtcacc gagcgctgcc
      121 caaagatgcg cggcccgcgt cttacgattc tgcggatcga gcgtgaccaa gtgtctcagt
      181 acctcgtcca gaccatacag cgtactgtct tcgagcggtt cccaggaaga catgctttcg
      241 cagccaaccg cgatgcgcat gtagcgcagt tcttcccatg taaagtttgg cgtaaccggg
      301 atcggccgca ggcacgggtt tcctccgctg gcttcaagca acttcctcac ctcatcgcca
      361 cgaagagata aacggctgtt gtcgaccagc agaacccctc taacaccaga gaaaagctcc
      421 gtcagccgct ccgtggccaa gtaggtctcg cccggtctcg acatccttcg agagcaatca
      481 cccgcgtcga ctgcaatcac gaacggcgtc tgccgtagtg ccgccaccag cttcttgcgt
      541 tggctatctg aatcggtctt gaatgcagcg aggatccgac ggatgtcgac gtggtactct
      601 tcttccccga catttgtccc gtcgacgcag tacttgggca gaaggttccg aacaacgtcg
      661 tccacaggat caggctcggt caggccgagc gttcgcagga acgcccgcga ctcctccgta
      721 ttgcacacgg ctggatgcac ggtgggaaag cccgtttcga tagctcctgg cagaaacgcc
      781 tgcggctgac cgttggcatg ggctcggacg tgcttaccgt ctgtgagccg gatcagtggc
      841 agctccttcg ccctccaaag caacgccttt tgaccgccga ggaactcgta taaacggcgg
      901 acccattctt cgggctgcgc ttccagaaac atcctattta acttcggcaa gatcgtctcg
      961 ggtcgtacct cctcgatatc gagttcatca atcaggtaat cccgcagatc cggtgaacgg
     1021 tccggcgaga tgtcgccgct cagccatccc aattcgccat cggcaccgag caaggcagcc
     1081 agttgcgctg gactgaacaa ctcccgcagt tcctgggtcc gcgccatggt agcctgtctc
     1141 gccgcgacgt gaccgccgcc ataacgcggc aggagcggct cgttcgcaag cgcctctttg
     1201 gtcgcctcga atatcggtgc gaacatcgtg cgctcgccaa acttcgagcg atccaatggg
     1261 aggcagcgca acacgccgac atcaagcaga tccttgtcac gcagccaatg cagggcttcg
     1321 agtaaaacgg ttgccgtttc attcacgcaa taccggttcc acgcgtcgtg aggggggacg
     1381 ttgtcgcggc tcggcgtggt gcgatagggg ccctgcacca ggaatccgag gtacgtgtcc
     1441 accacagtcg ggaagaacac gaccagccgc gagcgtgaaa cagggtgtaa cctctgttgt
     1501 ccggacttgt cgtctttggc cagagagaac gcgatttcga cgtaacctgc ttggttgcct
     1561 tccggtgtac agacaggctt ggagaacacc agccaggact cctcgacatc cggatgcccc
     1621 tccgcttggc cgatcaccgt gatgcgccgt accccatcac caaggcgctc cggctggctt
     1681 cggaggtaca ggccggaagg accgccatcg acgctccact cgatctcctc gatttcgcgt
     1741 aggaacagga gagtgctggg gccgaggcgg ttgagcccag cttcgatctc aaaatcaaaa
     1801 ccctcatcag acggcttccg cgggatgagt atgacggtct cctcgacctc gcggtcgctc
     1861 ggcgatatgg catatggccg aatgtagtcc tcgatagcaa agtcctcttg tcctgaatgg
     1921 atctgaggcc tatccgtgaa cgcatacaca gacttgaacc caatgccgaa gcgtccaatg
     1981 tcagtcagct ccttggtggt ctcgcccaca ctgcaaatcg cttcaacatc cgcttgatcg
     2041 aacggcttcc cgcaatgaat cacgcgtagg ctctgttcgg aaagactgaa tttcaccgct
     2101 ctagaaccgt accatccatt cggtcgtctc ctcagcgcat cctcagcgtt ttggagtaat
     2161 tcaaatatga aatgtaccgg atccgcatat agggtggata gcgtacgaat gctgtttcgt
     2221 gcggtccgct caactcgctc tcgatcgtta gcgattacct cgtacctagg agcctgtcgg
     2281 agaaatgctc tcaggcccat ttgagcaact tttcgagggt cctgaaatgg aggatcgagc
     2341 cgagatcgtc gatcctgagc caaatgtgag ggtcgttttt cttatgcgcg atcgctaact
     2401 cgaata
//
LOCUS       DATMXI010000254        10496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_177602, whole genome shotgun sequence.
ACCESSION   DATMXI010000254 DATMXI010000000
VERSION     DATMXI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177602"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VN444_04795"
     CDS             <1..1131
                     /locus_tag="VN444_04795"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWR21163.1"
                     /translation="ILKRDVDTYRQIYSGILERQKQAGVSQGLAASNVQIVDTAEAPM
                     VPFSPNKTRNILLGIVLGFTVAIGMAFFFDYLDNTVKQPEELERTLGIPNLALIPSLA
                     SAIPRQERGRLSRNGSNGDHQNGKPVFALVAHSDLRSTLTEAFRTLRTSLLFSSPGSP
                     PKSIMFTSAQPSEGKSGLSINTAITLSQLGGSVLLIDGDMRRPTCHTHLELPLKSGLS
                     DYLTGNADLVSIIKRTTVPNLHCIPAGTIPVNPAELLASPRMKETIDLLSQRFDYIIV
                     DSPPIFGVADALILSTVVKGVILVVQGGRTPREMVQRAFKNLIELNAPVLGAVLNNVD
                     IRGNGYPYYYHYHYSHYYQQTPADAAHLPDINEITKETKQTI"
     gene            1135..2418
                     /locus_tag="VN444_04800"
     CDS             1135..2418
                     /locus_tag="VN444_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21164.1"
                     /translation="MVIRLPARPSRIALSGILVLGFAFASWSALRPWIAQRVAGVEPD
                     ENATRHALTIDPDNDRIHAIRATLFHYSLLLRDYPAALTAYRSILRNNPLDSASWLHL
                     GKLYERLGQPSEADQAFRLATHLGPGNSALLWEIAVAYLDQEQTPKTLDTLARFLSAA
                     NNPADLAKGHELARRLASPDEVLEKMIALDIDHYTHYANYLLDRNLGDQALAVWDRLH
                     EMAARTDESIDSDLQLRIVDLLMATGKLDQAHQLWATLMSQMKPGAALTAANLVSNGN
                     FEHKETIGRGFDWRIGGAPGVAWTLDSDMAYSGRRSLRLSFLKSRADFSNLSQLIPVQ
                     PYSMYALQAHIKTNGFAGAKGITVEVIDSAEGILATTEAIGGTRNWSPVAVKFRTSGA
                     ARTITLRVRSEPPPPYLPALSGSAWIDNVSLMKVE"
     gene            2455..3795
                     /locus_tag="VN444_04805"
     CDS             2455..3795
                     /locus_tag="VN444_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWR21165.1"
                     /translation="MPRTRQNLKSVNPDEAGCLQLIEALADGNPIPDISTICQDRQRL
                     IALLAEISTGERSELFPSTRQVAERRGLSAHEIASRAAFLLACLDTPGADDPYTILGV
                     DPTATDAEIKEAWLSRLSLYHPDRHPEHSDWFTRHAARLNEAYHTLKDPTRRHAYDER
                     RRRELLARERGSPFAIQPDSSIPSPMPSQPSRPARHRVPALIATASLAAAGLVLMALS
                     TRPPAGPQLYLETVQPVAAVTLPSPTPINNPLPARLIRGGPPSPNAPVTRPVDHQPER
                     RLITRSQQVSLPVGLPAGLSNSSDDSAVSERSAADQILLAQALPPIVPEPKGLDRQEI
                     DALLDEYVDAYEKADVERVMATLSTRVREKGTLDYQTIRNAYIKGFTGRDQIIYRLKN
                     LQVEIKGEQATVTAQYLISARNAAQSTKGTTVSGRIEWKIQRESDKLKIIAINY"
     gene            complement(3926..5185)
                     /locus_tag="VN444_04810"
     CDS             complement(3926..5185)
                     /locus_tag="VN444_04810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWR21166.1"
                     /translation="MYDILIIGGGPAGLYAAHCLAKAGRSVAVFEEHQKIGEPAHCTG
                     LMATESFSRFNLPREAIRATLGSARFHSPAGYMLSVASEQELAVVVDRAAFDQGLADQ
                     AVAAGADIFLGHRVEALRRFRRSLVARTSHSGGVGRLVRGRLGILATGASYGLHRGLG
                     LGLPGRFVHGVQVEVKFEETSEVEVYFGNEVAPGSFAWVVPFTRQGVAMAKIGVLSSR
                     EAVRYLTRFLRSPQVASRVRPGRVCPYLRRPVPVWPLSGTFADHVLAIGDAAGLAKPT
                     TGGGVYYSLLSAELAAATIGEALDSGDGSARFLSRYQTAWRAVLGSEIRTGAMFRTFA
                     SQLSDAQIDEAFHLVAREPMARLIRDHAAFNWHRSIILALWRSSGVRGFLWRVLMRRG
                     GQMIGTLRPPFQASPAIQLPEETAAAD"
     gene            complement(5222..6334)
                     /locus_tag="VN444_04815"
     CDS             complement(5222..6334)
                     /locus_tag="VN444_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWR21167.1"
                     /translation="MPHLYDRTTRFVTLARDWLLPLLWLGTLIAVGNADLVPVRGTDT
                     PLFALRKLIHVGEYAILSVLICLALALDRQQVGRRLGIIALTLTVASGGIDEWQQSFV
                     AGRAPRLTDLGFDTIGAALGLAWVSIVRQSRRTESLRHGYALNERRRVHMLSAKRGFD
                     LLLSGLGLVASAPLWGVIAAAIKLDDGGPVFFRDDRVGLNGRIFTAYKFRTMVHNADA
                     LFGPCQAIEDDPRVTRVGRLLRATAMDELPQLWNIFRGDMSFVGPRALRPGEIESRGS
                     RFNVQGSTCKVQDSVAEAVTPLEQIPGYSKRHVVRPGLTGIAQVFADRDIPSRQKFRY
                     DLLYIRRRSFWLDVRLVALSFWVTFRGRWEVRGTKF"
     gene            complement(6468..6641)
                     /locus_tag="VN444_04820"
     CDS             complement(6468..6641)
                     /locus_tag="VN444_04820"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWR21168.1"
                     /translation="MTGQDGSYLAEFLLARGYTVHGLIRRASSGAAADALPRKSRRQG
                     RVSARSRPGASAG"
     gene            complement(6694..6876)
                     /locus_tag="VN444_04825"
     CDS             complement(6694..6876)
                     /locus_tag="VN444_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21169.1"
                     /translation="MKELQPVDLYQFRHHLELWVAGDDRRVVSEGGGDGKGVSMGERI
                     TRFYPGWVYSPRLAAG"
     gene            7147..7371
                     /locus_tag="VN444_04830"
     CDS             7147..7371
                     /locus_tag="VN444_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR21170.1"
                     /translation="MRISKISSKGQVTLPKEVREATGLQPGDMIAYEVNDGLIILRHV
                     EPFDAAFHAALSNTLDEWATPEDEKAFRDL"
     gene            7361..7699
                     /locus_tag="VN444_04835"
     CDS             7361..7699
                     /locus_tag="VN444_04835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWR21171.1"
                     /translation="MISERWDVVVVPFPFSERPDTKRRPALALSALSFNNSGHTVLAM
                     ITSKAHPPWPGDTDIEALEPAGLNTRCMIRLKIFTLDNRLILKKIGRLSPGDRTGVAE
                     QIRRYLPVTQ"
     gene            complement(8296..8622)
                     /locus_tag="VN444_04840"
     CDS             complement(8296..8622)
                     /locus_tag="VN444_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWR21172.1"
                     /translation="MRPQSIAQQDSSAAVTPPIRPRMPWRVVEARPLDGFRLHVRFVD
                     GLEGEVDMSSLVRSPTAGVFARLADPSLFAQVFVEHGAVMWPGELDLAPDAMHAEIKK
                     EGKWVL"
     gene            complement(8577..8897)
                     /locus_tag="VN444_04845"
     CDS             complement(8577..8897)
                     /locus_tag="VN444_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21173.1"
                     /translation="MCAIAGFQGSFNAGLLARMSEAIAHRGPDGCGAFHRPDHGIGLA
                     HRRLAIIDLSHAADQPMCDETSGLAIVFNGELYNYRELRAELMEDWELCAHNQSPNKI
                     RPLP"
     gene            complement(8936..10126)
                     /locus_tag="VN444_04850"
     CDS             complement(8936..10126)
                     /locus_tag="VN444_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21174.1"
                     /translation="MLTRKLASTLQRLSGSFPVVVITGPRQSGKTTLARAAFADKPYV
                     SLEDPIERAFAEDDPRGFLTRFPDGAVFDEAQRWPDMFSHLQGMVDQARHPGMFILTG
                     SQQFGLLAGVTQSLAGRVGTTHLLPLSLAEIPAVREGSVSLDAVLVTGGYPALHAYEI
                     APTDWFSGYVATYVERDVRQVLNVQDLSAFQRFLRLCAGRTGQVLNLSALAGEAGISH
                     STARAWLSVLESSYLVFLLPPYHRNFGKRLVKTPKLYFVDVGLAAWLLGIRSVEVLEL
                     HPMRGALFETLVVGEFVKARYNAGLPADLFFWRDNNGLEADLVFEQGMRLQPVEIKSG
                     QTVTSDYIRAGQKSARFAGDKAAKPWLVYGGNESYERSGVMVMGWRALANDGGAHVTT
                     DSAE"
     gene            complement(10307..>10496)
                     /locus_tag="VN444_04855"
     CDS             complement(10307..>10496)
                     /locus_tag="VN444_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21175.1"
                     /translation="LGVFPPKLAARAVESRLLISGSHFPVPGAAGLRVLPVEMLIHLW
                     AWVREVGRHGIPMTSYTI"
BASE COUNT         2282 a         3368 c         2823 g         2023 t
ORIGIN      
        1 attctgaagc gtgatgtcga tacctatcgc caaatttata gcggtatcct ggagcgccaa
       61 aagcaagcgg gtgtctccca ggggcttgca gcgagtaacg tccagatcgt ggacacggcc
      121 gaggccccga tggtcccgtt cagcccgaac aagacccgga acatcctact gggcatcgtc
      181 ctgggattca ctgtggccat aggcatggcc tttttctttg actacctgga caacaccgtg
      241 aagcagccgg aggaactcga gcgcacactc ggcatcccga acctggcgct gatcccctcg
      301 ctggcttctg cgatccctcg acaagagcgg ggtaggctct cacgaaacgg cagcaacggg
      361 gatcatcaga atggtaagcc ggtctttgcg ctggtcgcgc acagtgatct gcgttccacg
      421 ctgacagagg cgtttcggac actccggacc tcgctcctct tctcctcccc cgggtcgcct
      481 ccgaagtcga ttatgttcac cagcgcccag ccttctgaag gcaagtccgg attgtccatc
      541 aacaccgcca tcaccctctc tcagttaggc ggcagcgtgc ttctcatcga cggcgacatg
      601 cggcgaccca cctgtcatac ccatctggag cttcccctta agtcaggcct ctcggactat
      661 ctgaccggta acgcggacct ggtctcgatc atcaagcgga ccacagtgcc gaatctgcac
      721 tgcatccccg caggaaccat tcccgtaaat cccgcggagc tattggcctc tccgaggatg
      781 aaggaaacga ttgatctcct ctctcaacga ttcgactaca tcatcgtaga ctcgcccccg
      841 atcttcggag tggcggatgc gctgatcctc tcgacagtgg tcaagggcgt gatcctggtg
      901 gtccagggtg ggcggacgcc acgcgagatg gttcagcgcg ccttcaaaaa cctcatcgag
      961 ctcaatgcac cggtcctggg tgctgtgctg aataatgtcg atatccgggg taacggctat
     1021 ccgtactact accactacca ttatagccat tactaccagc agacccccgc cgatgcggct
     1081 catcttcctg acatcaacga aataactaaa gagaccaaac agaccatata gaccatggtc
     1141 attcgcctgc ccgctcggcc atcacgaatc gcgctctcgg gcatcctggt ccttggcttc
     1201 gcattcgcct cgtggtctgc gctccgccca tggatcgctc aacgggttgc aggtgtggag
     1261 ccggatgaga acgccactcg gcatgccctg acgattgatc ccgacaacga ccgcattcat
     1321 gccatacgtg ctaccctgtt tcattacagc ctgcttctca gggattaccc tgcggcgctc
     1381 actgcctatc ggtcaatcct gcgcaacaat ccgctcgata gcgcctcctg gctgcacctc
     1441 gggaagctgt acgagaggct cggccagcca tcggaggccg atcaggcatt ccgtctcgcc
     1501 acacacctcg gcccgggcaa ctcagcgctg ctctgggaga tcgccgttgc gtatttagac
     1561 caggaacaga cacccaagac gctggacaca ttagcccggt ttctttcagc cgcgaacaac
     1621 cccgctgacc tcgcaaaagg gcacgagctg gcgcgcagac tcgcctcgcc ggacgaggtg
     1681 ctcgagaaaa tgatcgccct tgatatcgat cactatacac actacgcgaa ttatctgctc
     1741 gatcgtaatc tcggcgatca ggctcttgca gtatgggacc ggctgcatga aatggctgca
     1801 cggaccgacg agtcgataga ctcagacctt cagcttcgga tcgttgatct gctgatggcc
     1861 acgggcaagc ttgatcaggc gcatcagttg tgggcgaccc tgatgtcgca gatgaagcct
     1921 ggcgcagccc tcaccgcagc gaaccttgtg tccaacggta attttgagca caaagagaca
     1981 atcggcaggg ggttcgactg gaggatcgga ggcgcgccgg gagttgcctg gacccttgat
     2041 tccgacatgg cctactcagg gcgccgatcc ctcagacttt ccttcctgaa aagtcgtgcc
     2101 gatttctcaa acctctcaca actcatcccg gtccagccgt attccatgta cgccctccag
     2161 gctcacatca agactaatgg attcgccgga gccaagggga tcaccgtcga ggtgatcgat
     2221 tccgctgagg ggattctcgc tacaaccgag gcgattggcg ggacacgaaa ctggagtccg
     2281 gttgcggtga aatttcgaac atcaggcgcc gcccggacta tcacgcttcg agtccgcagc
     2341 gagcctcctc ctccctatct tcctgcgctt tcagggtcgg catggattga caacgtctcg
     2401 ctgatgaagg tagagtagcg tgcaacattc aaggttcaag gttcaaggtt aaaaatgcca
     2461 cgaacaaggc agaacctgaa gagtgttaat ccggatgaag cgggttgcct tcaactgatt
     2521 gaggcgttgg ccgacggcaa tccgattccc gacatctcga ccatctgcca ggaccggcag
     2581 cgactcatcg cccttctcgc agagatcagt acgggagagc gatcggagct tttcccgagc
     2641 acccggcagg tggcggagcg tcgagggctc tccgcgcacg agatcgcgtc cagggcagct
     2701 ttcctcctcg cctgcctgga tacccccggc gccgatgacc cgtataccat ccttggggtc
     2761 gatccgaccg caacggacgc ggagatcaaa gaggcttggc tcagtcgcct cagcctgtat
     2821 cacccggacc gtcacccgga acatagcgac tggttcacgc gacacgcggc gcgtttgaac
     2881 gaggcctacc acacactgaa agaccccacg cgacgacacg cctacgatga gcgaagacgc
     2941 cgcgagctgc ttgcgcggga gcggggcagc cccttcgcga tccaacccga ttcgtccata
     3001 ccgtctccca tgccgtctca gccttcgcgc ccagcgcgtc accgagtgcc ggcactcatt
     3061 gcgaccgcct cgctggcagc ggccggattg gttctgatgg cgctgtccac tcgccctccg
     3121 gccggaccgc agctctatct cgagacggtt cagcccgtgg ccgcggtcac ccttccgtcc
     3181 cccaccccga tcaacaaccc tctacccgca aggctgatca gaggcggacc tccttctccg
     3241 aatgcgcccg tcacccgtcc tgtggatcac caaccagagc ggaggcttat cacgcgctcg
     3301 cagcaagtct ccttgcctgt gggcctcccg gcgggtctat ccaactcatc cgacgactcg
     3361 gccgtctctg aacgttcagc agcggaccag atactactgg cccaggccct tccgcccatt
     3421 gtgcctgagc cgaaaggcct cgatcggcag gagatcgacg ccctgctcga cgagtatgtt
     3481 gacgcctatg aaaaagctga tgtggagcgc gtgatggcca ccctctcgac ccgggttcgg
     3541 gagaagggga ccctggatta tcagaccatc cgcaacgcct acataaaagg gtttaccggg
     3601 cgagaccaga tcatctaccg gctcaagaac ctgcaggtcg agatcaaagg cgagcaagcc
     3661 accgtgaccg cgcagtattt gatctcggcg aggaatgccg ctcagtcaac caaaggaacc
     3721 accgtatcgg ggcgtatcga atggaagatc cagcgggaga gcgacaagct taagattata
     3781 gcaatcaatt actgataggt tcgaggtgcg gagttcaagg ttcaaggttc aacgtacaaa
     3841 gttcaaatcc ataagtgagt gagaagaaca taccccgcgg acgtgacacc gtggggcatg
     3901 ttatttctcc tcccgtttcg ttcgatcagt ccgcggcagc ggtctcctct ggaagctgga
     3961 tggcgggaga tgcttggaag ggaggtcgca acgtgccaat catctggccg ccacgtctca
     4021 ttaacactcg ccacagaaag ccgcgcacac cagaactacg ccagagggca aggatgatgc
     4081 tcctgtgcca gttaaatgcg gcgtggtcac ggatgagcct ggccatcggc tctcgggcga
     4141 ccagatgaaa ggcctcatca atctgcgcat cagagagttg agatgcaaag gttcggaaca
     4201 tcgctcccgt ccgaatctcc gacccgagaa cggcgcgcca ggcagtctga taccggctca
     4261 agaaccgagc agagccatcc cccgagtcca acgcctcacc aatggtagca gccgccagtt
     4321 ccgcgctcaa taagctgtaa tagacccctc cgccggtcgt cggtttggcg aggccggcag
     4381 catcgccgat cgccagcacg tggtcggcga aggtccctga aagaggccaa acggggaccg
     4441 gtcggcgcag atacgggcag actcgacctg gtcgcaccct ggatgccacc tggggcgatc
     4501 gaaggaagcg ggtcaggtat cgtacagcct ccctggacga caggaccccg atcttggcca
     4561 tcgccacccc ttgccgcgta aatggcacaa cccacgcgaa cgatccgggc gccacctcat
     4621 tcccgaagta cacctccacc tcggacgtct cttcaaactt gacttcgacc tggacaccgt
     4681 ggacgaagcg accaggcaag ccaagcccga gcccccggtg cagtccgtaa gacgcaccgg
     4741 tggccaggat accaagtcgc ccgcgaacca gccgccccac gccaccgctg tgagaggtcc
     4801 tagcaaccag actccttctg aaccggcgaa gcgcctcgac tcggtggccc agaaagatat
     4861 cggcgccggc tgcgactgct tggtctgcga gcccctggtc aaacgctgca cggtccacca
     4921 ctaccgccag ctcctgctct gaagccacag acagcatata ccccgccgga gaatgaaacc
     4981 gagcgctccc cagtgttgcc cggatagcct cccgcggcaa gttgaaccgt gagaagctct
     5041 cggtggccat caatccggtg cagtgcgccg gctccccgat cttctggtgc tcttcgaaga
     5101 ccgccacgct tcgtcccgcc ttggcgagac agtgggccgc atacagccca gccggccctc
     5161 ctccaataat tagaatgtca tacatatgta aggtccaagg ttcaaggttg aacgtttatc
     5221 cttaaaactt cgttcccctg acttcccagc gccctcgaaa ggtcacccag aacgagagcg
     5281 cgaccaaccg gacgtccagc cagaagcttc gtcgacggat gtacaggagg tcataccgga
     5341 acttctgacg gctaggaata tcccggtctg caaagacctg cgcgatcccc gtaagcccag
     5401 gtcgaacaac gtgccgcttc gaatatccgg ggatctgctc caatggcgtc accgcctccg
     5461 ccactgaatc ttgaaccttg cacgttgaac cttgcacgtt gaaccttgaa cctcgactct
     5521 ctatctcccc cggtcggagc gcccgtggtc ccacgaagct catgtctcct cgaaagatat
     5581 tccagagctg aggcaactca tccatcgccg tggcccgcag cagccgacct actcgcgtca
     5641 ctcgcggatc gtcctcaatt gcctgacatg ggccgaagag agcatcggca ttgtgcacca
     5701 tcgtacgaaa cttatatgcc gtaaagatcc tcccattcag ccctacacgg tcgtcccgga
     5761 agaacaccgg cccgccatca tccagcttga tggcagcggc aatcacaccc cacagcgggg
     5821 ccgaagccac caggcccagc ccggacagga gcagatcaaa gccgcgcttt gcgctgagca
     5881 tatgaacccg gcgcctctcg ttcaaggcgt atccatgacg cagagactcc gttcgccggc
     5941 tctgtcgaac gatggaaacc caagcgagcc caagagcggc cccgatggtg tcgaaaccaa
     6001 ggtccgtcag gcgaggcgca cggccagcta cgaaggactg ctgccactca tcaatccctc
     6061 ctgacgctac ggtcagggtc agggcgatga ttcccagtcg acgccccacc tgttgtctat
     6121 ccaaggcgag ggccagacag ataagaacgc tcaggatcgc gtactctccc acgtgaatca
     6181 gcttacgcag cgcgaacaag ggggtgtcgg tccccctcac cggcacgaga tcggcattcc
     6241 ccacggcaat gagggtcccc agccagagga gcggcaggag ccaatctcgc gcaagtgtca
     6301 cgaatcgcgt ggtacgatca tagaggtgtg gcatcaccgg ccatatcatc tattcctagc
     6361 cattcctacc catcaggcca gattaatata ccattttatg ctacctatta ctactgatta
     6421 ccactaatca ctctttttcc acctatttgt ctttccttag ggcctcctca gccagccgaa
     6481 gcgcccggcc tcgatcgcgc gcttaccctc ccctggcgtc gagatttccg aggtagagcg
     6541 tctgcggccg ccccgctgct cgcgcgccgg atcagcccgt ggaccgtgta gcccctggcg
     6601 agcaggaact cggccaggta cgagccgtcc tgacctgtga tgtcgggatg agagccacct
     6661 ttggcatgag gcctcctgtg gtgaagtaac gaactacccc gcggcaagcc gtggggaata
     6721 gacccacccc ggataaaagc gcgttatccg ctctcccatg ctgacacctt taccgtcgcc
     6781 accgccctcc gaaacgacgc ggcgatcatc accggcgacc cagagttcaa ggtggtgacg
     6841 aaactggtac aaatcgactg gctgtagctc cttcatctgt cctgccattt cccctacatg
     6901 tctttctccc ttcctccctc cgcccttaaa gactgcatcg acctctcaat cgccactgcc
     6961 acctcatgta tgacaaccca cttgagggcg gaggtgtgag gcttgaggcc tcatgttgga
     7021 ggccactcgc aaggaatttc tgagcccgtt gatctttctc ttcatggcct cattgccacg
     7081 catcccaact tgacaagaag ggctatgtat cgtatcttta ctctaaagag taaaggaggt
     7141 tttaccatgc ggatcagtaa gatttctagc aagggtcagg tcacgcttcc aaaagaagtt
     7201 cgtgaggcta ccggtctgca acccggtgac atgatcgcct atgaggttaa tgacggcctg
     7261 atcatactca gacatgtcga gccctttgac gctgcctttc atgccgccct ctccaacaca
     7321 ctcgacgaat gggccacccc tgaagacgaa aaggcattcc gtgatctctg aacgctggga
     7381 tgtggtcgtc gtccccttcc ccttttccga gaggccggac acgaagcgac ggcccgcgct
     7441 ggcgttgagc gctttgtctt tcaataacag cggccacact gtccttgcga tgatcacgag
     7501 caaagcccac cccccatggc cgggagatac cgacattgag gcgcttgagc cagccgggct
     7561 caacacacgc tgcatgatcc gcttgaaaat ttttaccctc gacaacaggc tcatcctgaa
     7621 aaagatcggc cgcctctcac cgggcgaccg caccggggtc gctgagcaga tccgtcgcta
     7681 cctccccgtg actcaatagc caatccgtca tacccgatgc ttttcctgcc gtagcgttcc
     7741 gtccagaaac cccgcccagt gcttcacccg acggaatcgc tcttcctcga cggcggctac
     7801 aagctcgcca tcgtgcagaa gcacggctga cgcgcctttt caacgaagtt ggctctccca
     7861 aactccacga gcatccgctc cgtgccggcg gagatcagcg atcgcgtggt gcggatcaac
     7921 aactgacccg tgcccacacg tgggcaaggc acttcagcca gttcagtgac gccgctattc
     7981 aagtttcgga ggatttgttt catccaaacc ttagtgctct ttgtggcttg gtgggagcct
     8041 taccctcgtg tcatttcttg atcagccgca gatcggtaag gaatggctgc agtttgccga
     8101 tctcgatctt ggcgaaagcc gggtgcaggg gatggagtag gtagtaaccg tctcacaata
     8161 atcactgtac gtgatcgaag aatccctcct gacctctctt tagcaaagag gggtaggggg
     8221 agattttctt aatcgatgtc aattcaatgt tgagacggtt aatatatggc agcatgctga
     8281 cgttcgtacc attgcttaca ggacccattt cccttctttc ttgatctcgg cgtgcatggc
     8341 atccggagcc aggtcgagtt caccgggcca catcacagca ccatgctcca caaagacctg
     8401 tgcgaacagg gaaggatcgg caagccgggc gaagacgccg gcagtgggcg aacgcactag
     8461 cgatgacata tccacttcac cctccaggcc atcgacgaag cggacgtgca agcggaagcc
     8521 gtcgagcggt cgcgcctcga ccacacgcca cggcatccgt ggccgaatgg gcggcgttac
     8581 ggcagcggac gaatcttgtt gggcgattga ttgtgggcgc atagttccca atcctccatc
     8641 agttcggcgc gaagttcccg gtagttgtag agttcaccgt tgaagacgat agcaaggcct
     8701 gacgtttcgt cacacatggg ctgatcggcg gcgtgggaca gatcgatgat ggccaggcgc
     8761 cgatgcgcaa gcccaatgcc gtgatcaggc cggtggaagg cgccgcagcc gtccgggcca
     8821 cggtgggcga tggcttcgct catcctcgcc agcaaaccag cgttaaaact cccctgaaac
     8881 ccagcgatcg cgcacacgat ggatttatcg tctcaacact ttgccgcggc gattctcatt
     8941 ccgcgctgtc cgtcgtcacg tgtgccccac catcattggc cagcgcccgc catcccatca
     9001 ccatgacccc gcttcgttca taggactcgt tgcccccgta caccagccac ggcttggcgg
     9061 ccttgtcccc ggcgaatcgc gcggactttt gccccgcgcg aatgtagtcg ctggtcaccg
     9121 tttggcccga cttgatttca accggctgaa ggcgcatgcc ctgctcgaac accaggtcag
     9181 cctccaggcc gttgttgtcc cgccaaaaaa acagatcggc tggtagccca gcgttgtaac
     9241 gggccttgac gaactcgccg acgacgagcg tctcgaacaa ggccccgcgc atcggatgca
     9301 gttccagaac ctcgacggaa cgaatgccca acagccaggc ggccagaccc acatcgacaa
     9361 aatacagctt gggcgtcttg accaggcgct tgccgaagtt gcggtgatag ggcggcaaca
     9421 ggaacaccag gtagcttgat tccagcaccg acagccaggc gcgtgcggtg ctgtgcgaga
     9481 tgccggcctc gcccgccagc gcactgagat tgagcacctg tccggtccgt ccggcgcaca
     9541 agcgcaagaa gcgctgaaac gcggacaggt cctgcacatt caacacctgc cgcacatcgc
     9601 gctccacata ggtcgcgacg tagccggaga accagtccgt cggcgcaatc tcatacgcat
     9661 gcagggcggg gtagccaccg gtcaccagta cggcgtctag tgaaactgac ccctcccgca
     9721 cggctggaat ttccgcaagc gacaacggca agaggtgtgt cgtcccaacc cgccctgcca
     9781 gggactgggt cacaccggcg agcaaaccga actgctgcga accggtcagg atgaacatcc
     9841 ccggatggcg cgcctggtcc accatcccct gcagatgcga aaacatgtct ggccaccgtt
     9901 gcgcctcatc gaacaccgct ccgtccggga accgcgtcag gaaccccctc gggtcatcct
     9961 cggcgaaggc gcgttcgatc ggatcttcca gactcacata aggcttgtcc gcgaaagccg
    10021 ccctggccaa ggtcgtcttg cccgactgac gcggcccggt gataacgaca acaggaaaac
    10081 tgccggaaag gcgctgaagc gttgaggcaa gtttacgagt tagcatgtta ttaacggtct
    10141 cgacatagga tttaccttga ttggaaaatc tcccctcgcc cctcttttcc aaagaggggt
    10201 aaaatcctcc ctttgacaaa gggaggtgtg gagggatttt acagatagtg tcgttattat
    10261 tatgagaccg ttattaaagt aattatacaa atagacgacg taatagtcaa atagtataac
    10321 tcgtcatggg aatgccgtgc ctacccacct cacgaaccca tgcccacagg tggatcagca
    10381 tctcaaccgg tagaacacgt aagccagcgg ctccaggaac gggaaaatga ctacccgaaa
    10441 taagtaatct ggattcaacc gcccgcgcag ccagctttgg cggaaagaca cccagc
//
LOCUS       DATMXI010000255         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_228633, whole genome shotgun sequence.
ACCESSION   DATMXI010000255 DATMXI010000000
VERSION     DATMXI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228633"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VN444_04860"
     CDS             <1..592
                     /locus_tag="VN444_04860"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWR21176.1"
                     /translation="TQVLIGTQMVGKGHDFPGITLVGVLSADASMVLPDFRAGERTYA
                     LLTQVAGRAGRGNRPGHVLVQTYNPDHYSILAARNHDYEALYRIEQPLREKRSLPPFG
                     FLVLLLVTSSNEGHAQEKAERLANLLLKRAVSSLTVEGPAPAPLYRLKGRYRWQILAK
                     GPDPSTLHHWVKETITLLPPSEQAGIEVDVDPVDLC"
     gene            705..1559
                     /locus_tag="VN444_04865"
     CDS             705..1559
                     /locus_tag="VN444_04865"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HWR21177.1"
                     /translation="MSGAIQQGTVERVSSMVCHASTIPAPQTDLWLGKGESLDDLGIS
                     GLKVIQSLTGFRHSMDALLLAQWATPRSTDRILDLGCGNGAIALLLAHRYPRAHIVGI
                     EIQSALADRARRGARLNGLQSRIEIVEGDLRQIKGLLPSAGVDMVLCNPPYRELGRGR
                     LSPDPEVRQAKHELTATLQEAVAAIRYVLAPKGRACLIYHASRLVELLSDLRVNRLEP
                     KRMRLIYSYPGAEAELSLIEARRDGRPGLQILAPLFVYQARGGPLSPSMEAIYRSLAL
                     SDVRSGEA"
     gene            1556..2413
                     /locus_tag="VN444_04870"
     CDS             1556..2413
                     /locus_tag="VN444_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWR21178.1"
                     /translation="MSRHIIALAQISPALGDLERNLALHEKTAEEAIGRGAGLLVFPE
                     LSLTGYFLKDLVSSVALPMTSPILGRLRDLSRRIDLVVGLVEESPEHLFYNAAIYLSM
                     GEIQHVHRKVYLPTYGIFDEQRYFAEGGRIRTFRARVGRSAILICEDMWHPSAAYVAS
                     LDGMEILISPSASPGRGGLEEGKFFANARAWETINRAYAQLFTCYVLFANRVGYEDGA
                     CFWGGSEVIAPSGEPVTKAEYLSEEILVVEIDPTEVRRARTVNPLLRDERLDVTLREL
                     ERVKREGVR"
     gene            2410..>3157
                     /locus_tag="VN444_04875"
     CDS             2410..>3157
                     /locus_tag="VN444_04875"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWR21179.1"
                     /translation="MSGLSLHCEFVQQILTGFIANEVRKTGFSRVVVGLSGGVDSALS
                     AYLGVEALGAENVLALLMPYRTSNPESREHAELVVKQLGIQSDVIEITPMVDAYFERF
                     PESDQIRRGNKMARERMTILFDHSAKLKALVLGTSNKTELLLGYGTQYGDMASAINPL
                     GDLYKTQVRHLARHIGIPEIIVRKAPSGDLWVGQTDEAELGFTYEEVDRVLCLMVDRR
                     CEIPELIAAGFDERFVRTVLARIQASQYKRR"
BASE COUNT          606 a          943 c          971 g          637 t
ORIGIN      
        1 gacgcaggtc ctgatcggga cgcagatggt tgggaagggg cacgacttcc ccgggattac
       61 cttggtcggg gtcctctctg ccgatgcctc gatggtactc cccgatttca gggccgggga
      121 gcgaacctat gccctgctca cacaggtggc cgggcgagct ggtcgtggta accggcctgg
      181 tcatgtgctg gtccagacct acaacccgga tcactatagc atcctcgcag cccgcaacca
      241 cgattatgaa gcgctgtacc gaatcgagca gccactcaga gagaagcgca gccttccccc
      301 cttcggcttc ctcgtcctgc tcctggtcac ctcttcaaac gagggtcacg cgcaagagaa
      361 agccgaacga cttgccaacc ttctcctgaa acgggccgtt tcgtccctta ccgtcgaggg
      421 accagcgcca gcgccgcttt accgactgaa aggacgctat cgctggcaga tcctcgccaa
      481 aggtcctgac ccgagtacgc tacatcactg ggtcaaggag acgattacgc tactcccgcc
      541 gtcagaacag gcgggcatcg aggtcgatgt cgaccctgtc gatctatgct aagcgagcag
      601 cgaccagcca tcagcgttca gcttctctgg ctgactgctg accgctgata gctgactgct
      661 gaccatttgt cttttcctga gggatgcatt acagtaatgg cacgatgagc ggagcgattc
      721 agcagggaac ggtggaacgg gtttccagta tggtctgcca cgcgagtacg attcctgccc
      781 cccagactga tttgtggctt ggcaagggcg aatcgctgga cgaccttggc atctcgggtc
      841 tgaaggtcat ccagagtctt acaggcttcc gccattcgat ggatgcgctg ttactggcgc
      901 agtgggccac accgcgatcc accgaccgaa tcctcgatct cggttgcgga aatggggcga
      961 ttgcgctgct gctggctcat cggtatccgc gcgcccacat cgttgggatt gagatccaat
     1021 ctgccctggc tgaccgcgcc cggcggggag cgcgactcaa tgggttgcag agccgcatcg
     1081 agatcgtgga gggcgacctc cgtcagatca aagggctgct gccgtcggcg ggtgttgata
     1141 tggttctctg caacccgccg tatcgagagc ttggccgagg gcggctcagc cctgacccag
     1201 aggtccgaca ggccaagcat gagctgaccg ctacccttca agaggcggtt gcggccatcc
     1261 gatacgtgct cgcccccaaa gggcgagcgt gcctgatcta ccatgcctcg cggctcgtcg
     1321 agctgctgag cgacctcagg gttaatcggt tagagccgaa aaggatgcgg ctgatctatt
     1381 cctaccctgg cgcagaggca gagctgagct tgattgaggc gcgacgagat gggcgaccag
     1441 ggctgcagat tcttgcccct ctgttcgtct atcaagcacg gggcggccct ttgtcaccca
     1501 gtatggaagc catctatcgc agcctggcct tgtctgacgt acggagtgga gaagcgtgag
     1561 ccgacacatc atcgcccttg cacagattag ccccgctctc ggcgatctgg agcggaacct
     1621 ggccctgcat gaaaagacgg ctgaagaggc gatcggccgt ggcgccggcc tgctggtctt
     1681 tcctgaactg agcctgaccg gatacttcct caaggacctc gtctcatccg tggcccttcc
     1741 catgaccagt cccatcctcg gtaggctcag agatctaagc cgccgcatcg atctcgttgt
     1801 gggactggtt gaggagtcac ccgaacacct gttctacaat gccgccattt acctctctat
     1861 gggtgagatc cagcacgtcc accgcaaggt ctacttgcct acgtacggaa tcttcgatga
     1921 gcaacggtat tttgcggaag gcggccgaat caggacattt cgcgcgaggg tcggtcggtc
     1981 tgcgatcctg atctgcgagg acatgtggca tccctcggcg gcgtatgtcg cctccctcga
     2041 tggaatggag attctcatct cgccttctgc cagtccgggg cgcgggggtc tggaggaagg
     2101 caagttcttc gcgaacgcca gggcctggga gacgatcaac cgggcctacg cccagctctt
     2161 tacctgctat gtcctcttcg cgaatcgggt cggttacgag gatggggcct gcttctgggg
     2221 cggctcagaa gtgatcgccc cttccggcga gccggtgacc aaggcagagt acctcagcga
     2281 ggagatcctg gtggtcgaga tcgatcccac agaggtacgg cgggccagaa cggtgaatcc
     2341 gttgcttcga gatgagcgat tggatgtcac gcttcgagag ttggagcggg tgaaaagaga
     2401 gggagtcagg tgagcggtct ctccttacac tgcgaatttg tccaacagat cctgaccgga
     2461 tttattgcta acgaggtccg aaagaccggg tttagccgcg tcgtggttgg gctttccggc
     2521 ggggtggatt cagcgctgtc cgcctatctc ggggtcgagg ctctcggcgc cgagaacgtc
     2581 ctggccctcc tgatgccgta ccggaccagc aatccggaga gccgggagca cgccgaactg
     2641 gtagtgaagc aactgggcat ccaatcggac gtcatcgaga tcacgccgat ggtggacgcc
     2701 tacttcgaac ggtttcccga gtcagatcag attcgccgcg gcaacaagat ggcccgcgag
     2761 cggatgacta tcctcttcga tcattcggcc aagctcaagg ccctggtcct ggggaccagc
     2821 aataagacgg agctgttgct ggggtacggc acgcagtatg gcgacatggc cagcgccatc
     2881 aatccccttg gcgacctgta caagacccag gtccggcacc tggcgcgcca cattggcatc
     2941 ccggagatta tcgtccgaaa ggcgcccagt ggcgacctct gggttgggca gactgacgag
     3001 gcggagctcg gctttaccta cgaggaggtc gatcgggtcc tctgtctgat ggtggaccga
     3061 cgttgcgaaa tcccggagct tatcgcagcc ggttttgacg aacgcttcgt caggaccgtt
     3121 ctcgccagga tccaggcctc ccagtacaag cggcgcc
//
LOCUS       DATMXI010000256         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_159699, whole genome shotgun sequence.
ACCESSION   DATMXI010000256 DATMXI010000000
VERSION     DATMXI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159699"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VN444_04880"
     CDS             <1..791
                     /locus_tag="VN444_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWR21180.1"
                     /translation="GGFAVRMGLQYVKGLGEDDWKRIEDARRIAPFTSLADFVRRTEL
                     DQGSLSALAEAGAFEGFGSFGVARDRPFDTVRPEPVEGTNGTHGRLDRRTALWEVLGF
                     KALETSLPTVVQETSPLFTLLDAVEEIAWDYRRTDHSPRGHPLAPLREVLQARGLPDA
                     RTVASMRHGSRVRYAGLVICRQRPGTAGGVTFMTLEDETGFVNVVLWPNVFKRHATLA
                     KTARFLGVTGTLQAEHGVVHLVADRLWHPRVHADLATAPSRDFH"
     gene            796..948
                     /locus_tag="VN444_04885"
                     /pseudo
     CDS             796..948
                     /locus_tag="VN444_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1752..2039)
                     /locus_tag="VN444_04890"
                     /pseudo
     CDS             complement(1752..2039)
                     /locus_tag="VN444_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2184..3320
                     /locus_tag="VN444_04895"
     CDS             2184..3320
                     /locus_tag="VN444_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21181.1"
                     /translation="MANPWVSVQKLAVVPTFNTQFDTGPVPSDWDSLVMQRVVYDPDP
                     MTGADRSLRSYLGAISYGLATLDAKLFPRAFSDGPGVIEAAWQSLPEGHGYPYVLCVI
                     PRDDGGKHRGGFFTTVNKNDVNAVARVAMFSGAAVLQQRQIMGVWAMEVLHAIARLPD
                     LYAKETNPYPDPPIDDYDNMCFNAGTHSCAYLKRAAGWLPGGAMVSHKGTGQNYSLHA
                     IGMSPPPPGRVSAVSIRSRTSNQSFIVEARLKVDLYEKGFAPLTTGKLEFRGLKEEGV
                     IVYEVTTPARLEQVKLIRSGLQVGQSYSNSAEGFTIRVTAAINGGMSIRITRTPDPRC
                     ADISEEIAEINKDLAEPGLDVDTRKGLLKQRGKLKDEAKRLGCP"
     gene            complement(3816..>4051)
                     /locus_tag="VN444_04900"
     CDS             complement(3816..>4051)
                     /locus_tag="VN444_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21182.1"
                     /translation="SPTSSVPLEDVTTRIQLAYGFAVTAPDAERDAPSPMACPHCGGH
                     LIYRMMILPQHDRLLRGSPAWPSARPLQEVSSG"
BASE COUNT          882 a         1131 c         1242 g          796 t
ORIGIN      
        1 cgggcggctt cgccgtgcgg atgggcctgc aatacgtgaa ggggctgggt gaggacgact
       61 ggaagcgaat tgaggatgct cggcgcatcg cacccttcac gtcgcttgca gatttcgtcc
      121 gccgcaccga gttggatcaa ggatctctga gcgccctggc cgaggcgggc gccttcgaag
      181 ggttcggttc tttcggcgtc gctcgggaca ggccctttga caccgttcgt cctgagcctg
      241 tcgaaggaac gaacggtact catggcaggc tggaccggcg gacagcgctg tgggaggtac
      301 tgggattcaa ggcactagaa acctcattgc cgacggtcgt ccaagaaacc agccccctct
      361 ttacactgct cgacgctgtc gaagagatcg cctgggacta ccggcgcacc gaccacagcc
      421 cccggggcca tcccctcgcg cccctgcggg aggtactgca ggcccggggg ctcccggatg
      481 ctcgcacagt tgcctcgatg cgccatggat cgcgggtgcg ctacgccggc ctggtcatct
      541 gtcgccagcg gcctggcacg gccggcggcg tcaccttcat gaccttggag gacgagaccg
      601 gcttcgtcaa cgttgtcctc tggccaaacg tttttaagcg gcacgccacc ctggccaaaa
      661 ccgccagatt cctcggcgtc accgggaccc tccaggccga gcatggggtg gtccacctcg
      721 tcgccgaccg tctctggcac ccgcgagttc acgccgacct cgctaccgcc cccagccgcg
      781 acttccattg atgctcgtta ttccttcgcc atccatctgc tagaagaggg tcacgatatt
      841 cggaccattc aagagttgct tggccatcgg gatgtgagca ctacgatgat ctacactcac
      901 gtgttgaacc ggggcggacg gggggtcaat agcccggctg atcggctatg gcgcggccga
      961 gataggccgc taattattag ggagataggc cgccaaagct ggcagtgttt atcgggcaga
     1021 atcggcttga ccggttgggt taagtgtggg agattacgag taaattcatt gttgagaagt
     1081 agagccgcct cgggagatag gccgccagcc tttatcagct tctcagcggc tcactaatag
     1141 ttaggctggc aagcgcccgt tatatacagg ttgggtgcaa tgaccatcat ctgcaaaata
     1201 gccggacatg tgtgggaagg atgtaaatgc atcaggtgtg gtgctctgca ggataacttc
     1261 cacttatggc gaggttgtaa ttgtacaaaa tgtggggtaa cacgacatga ttggcaaggt
     1321 tgtatctgcc gacggtgccg gttcgtagac cgacaacgaa tagtaaacgc gatataccag
     1381 aagttggcag ccaaacgtgt cgcgcacgaa gaaatcggat tcgtcctagg tgaggaacat
     1441 atacgacgag ctgatgaact agttcaaaca cacggggact ttgaagttgt ctaccatcct
     1501 ccagagggag aaataggcag gggcgcggat gtcgctgacc ttgggggaat gccgtggcta
     1561 gtagacccaa acaaaccagt atattggggt acacccccaa gcatggatgt tgtccctccg
     1621 attagggaat ctaatggagc atagctaaac cagcgtaggc tgggggcgaa aaggggacag
     1681 ctttatttat agcctcgcta gaaccgttgc gaattccctt gactggccta acattatggt
     1741 gctccccatt gtcaagacaa aaataggggt tgattaaggt ggatagcgag aggctgagat
     1801 ggggtagtgg taagccgctc cgtcccgtgg tagacctcac agggggtgcg gtagcccaat
     1861 gcctcatgga gccgttccgt gttatagaac aggaaatagt gccccaagct ctccttcgct
     1921 tccctgaccg tctggtaggc cttcaggtat acctcctcgt acttgaccga tcgccagagc
     1981 cgctcgacaa agatgttatc gaagacccgg ccgcgaccgt catgactgat caagatgccg
     2041 gcatgactca gtcgttcggt gaactcggcg ctcgaaccga ccggctggag cttggggctc
     2101 gtcgcgagcg acacgcggcc ggcggttcag cgccacgtta gaccgcttct agaaaatcga
     2161 atcaacacca caggaggtaa gtcatggcta acccttgggt aagcgttcag aaacttgcag
     2221 tcgttcccac tttcaacacg cagttcgata ccggccccgt acccagcgat tgggacagcc
     2281 tagttatgca gcgcgtcgtt tacgatcccg acccgatgac cggggcggat cgttccctgc
     2341 gctcttatct gggagcgatc tcttacggcc tcgctaccct cgatgccaag cttttcccgc
     2401 gcgctttctc ggacggcccg ggagtcatcg aagccgcatg gcaatcgctg ccggagggtc
     2461 atggctaccc gtatgtgctc tgcgtcatcc cgagggatga cggtggcaaa caccgaggag
     2521 gctttttcac cacggtgaat aagaacgacg tcaatgccgt cgcgcgtgta gccatgttct
     2581 cgggggccgc cgtgttgcaa cagcggcaga tcatgggtgt ctgggcgatg gaagtgctcc
     2641 atgcaatcgc ccgtctaccg gacctgtatg ccaaagagac caacccgtac ccggacccgc
     2701 ccatagatga ctatgacaac atgtgcttta acgccggtac gcatagctgc gcgtatctga
     2761 agcgggcggc tggctggctc cccggaggtg ccatggtgtc gcataagggc acggggcaaa
     2821 attactctct gcacgccatc gggatgtcgc ccccaccgcc gggccgggtt tccgctgtta
     2881 gcatccgctc gcgaaccagc aatcagagct ttattgtgga ggcgagacta aaagtggacc
     2941 tctatgagaa aggcttcgcg ccgctgacta caggaaagct tgagttccga ggactgaagg
     3001 aagagggcgt gatcgtctac gaagttacaa cccctgctcg tctggaacag gtcaaactaa
     3061 tccgctccgg cctacaggtc ggtcagagtt acagcaacag tgccgaggga ttcaccatcc
     3121 gcgtgaccgc tgccataaat ggagggatga gcatccgcat cacgcgcacc cccgaccccc
     3181 gctgcgcgga catttctgaa gaaatagcgg aaattaataa agacctcgcc gaaccgggat
     3241 tggatgtgga cacaagaaaa ggcctgctta agcagagggg caaactcaag gatgaagcca
     3301 aacgactcgg ctgtccttga gacgataagc aaatccacgg caaatatgcc gaaactcgtc
     3361 gcggcatgtg gcactgctat ggtcaggcag cctgaacaga gcagcatgag ataagcagaa
     3421 gaacggctgt cgagtgacca tgccgcatct gagaggcggt ctaaccaata aggattgaga
     3481 cgaccggcac ggtcgtttca atctcttgtt caagaagccc gttggtggcg tcggctcgtg
     3541 atcaataaga aggagattgc ttccgaggct ggcgggtagg gggatggttg atgaggcagg
     3601 aagcgactca gtgaggcctt ggggtgccct gtatcggcag tgaccaccgt gaaaacgagg
     3661 tctcggtacg tacgcgtgag cgccgcccac gatgacaaca gccagcaatc ctggtgccag
     3721 gcataggtgg tcctgtcccg ctcgacggtg ggtgccgagc agggcgcaag gtcaatgggt
     3781 gtagacggtt ccggctcgtg ccgagaattg ggtcgctacc ccgatgacac ctcctgcagc
     3841 ggcctcgctg atggccatgc gggagacccc cggaggagac ggtcgtgctg cggcaggatc
     3901 atcatccggt agatcaggtg tccgccacag tggggacacg ccatgggcga cggcgcgtcc
     3961 cgttcggcat cgggtgcggt caccgcaaag ccgtaggcca actggatccg tgtggtcacg
     4021 tcctcaagcg ggaccgacga ggtgggcgac a
//
LOCUS       DATMXI010000257         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_230379, whole genome shotgun sequence.
ACCESSION   DATMXI010000257 DATMXI010000000
VERSION     DATMXI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230379"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2705)
                     /gene="metH"
                     /locus_tag="VN444_04905"
     CDS             complement(<1..>2705)
                     /gene="metH"
                     /locus_tag="VN444_04905"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWR21183.1"
                     /translation="EAFWNSIAHMPLLSVGINCALGAKQMRPYIEELAQIAPVFLSCY
                     PNAGLPNAFGGFDETPAIMAADLGDFARSGWLNIVGGCCGTTPAHIEAIAAAVQDLQP
                     RIPPQPQPHTRLSGLEPLTICPGVGFVNIGERTNVSGSTKFAKLIRNGEYEAAVSVAR
                     QQVEGGAQIIDVNMDEGMLDSKEAMVTFLHLVATEPDIARVPIMIDSSDWSVIEAGLK
                     CVQGKPVVNSLSLKEGEQAFIQRARLIKRYGAAVVVMAFDELGQADTLQRKIEICARA
                     YRILTETAGLPPQDIIFDPNILTVATGLEEHNNYAVNFIEACRWIKANLLHCKVSGGI
                     SNISFSFRGNNLIREAMHSAFLYHAIQAGLDMGIVNAGQLTVYEAIPKDLLDLVEDVL
                     LNRRSDATDRLVRFAETVTQQGQVAGKDEAWRRGTVEERLTHALVKGIVEYIESDVEE
                     ARQQYDRPLQVIEGPLMAGMNLVGQLFGSGQMFLPQVIKTARVMKRAVAYLLPFIEAT
                     RLASDSKRNQRKVLMATVKGDVHDIGKNIVGVVLGCNNYEIIDLGVMVPCDTILKTAR
                     EQQVDIIGLSGLITPSLDEMAHVARELMREGLHVPLLIGGATTSRTHTAVKIAPLYDQ
                     PVVHVSDASRAVGVVGHLLSQEQRSAFVESNRVDQERVRQAHQDRELRALLTLTQARD
                     RKHPIDWGVVDIPKPSFTGIRVLDEFPLDQIVPLIDWTPFFHAWEVRGRYPEVLQKPK
                     AKELFEDGQRLLEQIVSQRWLIARAVYGFFPANSVDDDIELYTDDSRSQVLATFHTLR
                     QQLHKAEGQCNLALADFIAPRSTGLPDHLGAFAVTTGHGLDTLCKRYETSHDDYTSIL
                     AKALADRLAEAFAECLHRRVRAEWGYGVGERLSNEDLIRERYRGIR"
BASE COUNT          535 a          852 c          764 g          554 t
ORIGIN      
        1 cggatccctc gatacctctc tcgaatcaga tcctcgttgc tgagccgttc tccgactcca
       61 tacccccact ccgctcgaac ccggcgatgc aagcactccg cgaaggcctc cgccagacga
      121 tcagccaacg ctttggccag aatagacgtg tagtcgtcat gggaggtttc atatcgcttg
      181 cagagggtat cgaggccatg gccggtcgtt acagcaaacg cgccgagatg gtccggaagt
      241 ccggtcgatc tgggtgcgat aaaatcggcc agcgccagat tacactgccc ttcggccttg
      301 tgcaattgct ggcggagggt gtgaaaggtc gccagcacct gcgaccgcga gtcatccgta
      361 tagagttcaa tatcgtcgtc cacgctgttc gccgggaaga acccatagac cgcccgggcg
      421 atgagccagc gctgactgac aatctgctcc agcaagcgct ggccatcctc gaacagctct
      481 ttggctttgg gcttctgtag gacctcagga taccggcccc gcacctccca cgcatgaaag
      541 aacggcgtcc aatcaatcaa cggcacgatc tggtcgagcg gaaactcgtc cagcacgcgg
      601 atgccggtaa aggacggctt tgggatgtct actacccccc aatcaatggg gtgtttccga
      661 tctcgcgcct gggtaagcgt gagcaatgcc ctcagctcgc ggtcttggtg cgcttgtctc
      721 acccgctcct ggtcaactcg attgctttcg acaaacgccg atcgctgttc ctggcttaag
      781 agatggccaa cgacccccac ggcccgggag gcatccgaga cgtggaccac cggctggtca
      841 tagagggggg cgattttcac ggccgtgtgg gttctgctgg tggtcgcgcc gccgatcaag
      901 agcggaacgt gaagcccttc tcgcatcagc tcccgcgcga cgtgcgccat ctcatcgagc
      961 gacggggtga tcagcccgct cagaccaata atgtccacct gctgttcccg ggcggtcttg
     1021 aggatcgtgt cgcacggcac catcaccccc aggtcgatga tctcatagtt gttgcagccg
     1081 agtacgaccc cgacaatgtt cttgccgata tcgtgcacat ctcccttcac ggtggccatg
     1141 aggacttttc gctggttgcg cttactgtca gatgccaggc gcgtggcttc aataaagggg
     1201 aggaggtagg ccaccgccct cttcatgaca cgcgcggttt tgatcacctg cggcaagaac
     1261 atctgcccgg agccgaagag ttggccgacg aggttcatgc cggccattaa cggaccctca
     1321 atgacctgaa gcggcctgtc atactgctgc cgcgcttctt ccacgtccga ctcgatatac
     1381 tccacgatcc ccttcaccaa cgcatgcgtg agccgttctt cgaccgttcc ccgtcgccag
     1441 gcctcgtctt tgccggcaac ctgaccctgc tgtgtcacag tctccgcaaa cctcaccagc
     1501 cgatcggtcg catcggaacg gcggttgagc agcacgtctt caaccagatc gagcaggtct
     1561 ttaggaatcg cctcgtagac cgtcagttgc ccggcattca caatccccat atccaggccg
     1621 gcctggatgg catggtacag gaatgccgag tgcatcgcct cgcggatcag attgttccca
     1681 cgaaacgaga acgaaatgtt gctgatcccg ccgctgacct tgcagtgaag caggttggcc
     1741 ttaatccagc ggcaggcctc aatgaagttc accgcgtagt tgttatgctc ctcgagcccg
     1801 gtcgccaccg tcagaatgtt cggatcgaag atgatgtctt gtggtggcaa cccggccgtc
     1861 tcggtcagaa tccggtatgc gcgggcacag atctcgatct tgcgctgcag tgtgtcggcc
     1921 tgaccaagct catcaaaggc catgaccacg accgcagccc catatcgttt gatgagccgg
     1981 gcccgctgaa tgaatgcctg ttcgccctct ttgaggctga gtgagttcac caccggcttg
     2041 ccctgtacgc acttcagccc cgcctcaatc accgaccagt cggagctatc gatcatgatg
     2101 ggcacccgcg caatgtcagg ctcggtggcg accaggtgca gaaatgtcac catcgcctct
     2161 ttcgagtcca gcataccctc atccatgttg acatcgatga tctgggcgcc gccctcgacc
     2221 tgttgccgcg ctacggagac ggcggcctcg tactcgccgt tccgaatgag cttggcaaac
     2281 ttcgtggacc cgctgacatt ggtccgctcc ccgatattga cgaaacccac gccgggacaa
     2341 attgtcaatg gctccagacc gcttaatcgg gtgtgcggct gaggttgagg gggaatgcgc
     2401 ggctgcaagt cctggacggc ggccgcaatc gcctcaatat gagccggggt cgtgccgcag
     2461 cagccaccga cgatgttgag ccagccgctc ctcgcaaagt cgccgagatc agcagccatg
     2521 atggccggtg tttcatcgaa tcccccgaat gcgttgggta agccggcgtt gggatagcag
     2581 ctcaggaata ccggggcaat ctgagcaagc tcctcgatgt acgggcgcat ctgcttcgcc
     2641 ccaagcgcac agttgatgcc tacagagagc agcggcatgt gggcaatgga gttccaaaaa
     2701 gcttc
//
LOCUS       DATMXI010000258         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_67641, whole genome shotgun sequence.
ACCESSION   DATMXI010000258 DATMXI010000000
VERSION     DATMXI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67641"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VN444_04910"
     CDS             complement(<1..142)
                     /locus_tag="VN444_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HWR21184.1"
                     /translation="MGRRLTAEAIGTFWLVFIGCGSAVLSASIPQLGIGVFGIAFAFG
                     VAV"
     gene            330..920
                     /locus_tag="VN444_04915"
     CDS             330..920
                     /locus_tag="VN444_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR21185.1"
                     /translation="MSPTSSGFSMLVLSGDPEIQSQFKQAFKDVSVTVAKDMSALSKD
                     MMRRSFDAVIVESKPGSSDRTGTNPPNIDLSHTMVITGSRTVLKRASRFMQMMVRQNS
                     ARPNGKDALSLEDYLEWKMGDFVKGMRHGSGRNLHPMLITAIERPLITRALQETKGNQ
                     IQAAELLGLNRNTLRKKIHDLHIPVKRSRTVKIREA"
     gene            998..1270
                     /locus_tag="VN444_04920"
     CDS             998..1270
                     /locus_tag="VN444_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWR21186.1"
                     /translation="MTKEELIAKMAASAGITKVAAGTALQAFTGAVTTSLKKGQRVSL
                     VNFGTFTVARRKARIGRNPRTGEALRIPAAKVPKFSAGKILKSAVR"
     gene            1572..2390
                     /locus_tag="VN444_04925"
     CDS             1572..2390
                     /locus_tag="VN444_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWR21187.1"
                     /translation="MKQYRVKIGRKLSLKQHDPDGTGNYKKDDDGRASAKAETDKHIA
                     TISQLQERLYANSARALLIVLQGMDTSGKDGTIRSVMSGVNPQGCKVVSFKAPSSQEL
                     AHDFLWRVHREVPAKGQIGVFNRSHYEDVLITRVHGWVSDEVVERRFEQIKEFEELLT
                     ENGTTILKFFLHISKKEQKERLEERVRDPEKRWKFNEGDLEERRLWNRYMVAFEDMMA
                     ATSTAHAPWYIVPANRKWYRNLVVAGRVVEAMEDMKLKTPPPSPGVNFDTLKIV"
BASE COUNT          609 a          619 c          689 g          531 t
ORIGIN      
        1 ctacggctac cccgaaggcg aacgcgatcc caaagacccc aatgccgagc tgtgggattg
       61 aggcgctcaa gaccgcgctc ccgcagccta tgaataccaa ccagaacgtg ccgatcgctt
      121 ctgccgttag tcgtcttccc atcagtgcct ccacgcaaga ggtttgctgc ccgcactatg
      181 cccgtgggag cgaaaaagcg caagcggcga aatgcccaca tcctgttccc tgtgcccatg
      241 ggaggctggc cagacatttt cctcattgac atcaaattgg ccctgggcaa taatccgcac
      301 ctcttctttc cttttcttct ggaacgttca tgtcgcctac gtcatctggt ttcagtatgt
      361 tggtgctcag cggtgatccg gagatccaga gccagttcaa acaggccttc aaagatgtgt
      421 ctgtgactgt ggcgaaagat atgtccgcgc tgtcgaagga catgatgcgt cgatcgtttg
      481 atgcggtgat cgtcgagtcg aaaccgggct catcggatag aaccggcacc aatcctccca
      541 acatcgatct gtcacatacc atggtcatca ccggttctcg aaccgtcctg aagcgagcct
      601 ccaggttcat gcagatgatg gtccggcaga attcagctcg accgaacggg aaagacgctc
      661 tgtcgcttga ggactatctt gaatggaaga tgggagattt cgtgaagggg atgcgacatg
      721 gttcaggacg caacctgcat ccgatgttga tcaccgccat tgaacgtccc ttgatcacca
      781 gagctcttca ggagaccaag ggcaatcaga ttcaggcagc agaactgctg ggtttaaatc
      841 gcaatactct caggaagaag atccatgatt tgcatattcc cgtgaaacgg agtcggactg
      901 tgaagatacg ggaggcctga ctcatgtcat gtggtggggt acagcgagcg tggtaacaaa
      961 gagttgagta gtttaaccca ataacaggag gggcatgatg acgaaagaag agttgattgc
     1021 aaagatggcg gcatcggctg gtattaccaa ggttgcagca ggcactgcgt tgcaggcttt
     1081 caccggagcc gtaaccacct ccctgaaaaa gggacaacgt gtctcgctgg tgaattttgg
     1141 aacctttacg gttgccaggc gaaaagccag gatcggacgg aaccctagaa ccggtgaggc
     1201 gcttcggatc cctgcagcca aagtccccaa gttctcggca gggaagatct tgaagtcagc
     1261 cgtcaggtaa catcgcacga tcgtcagatc gtatgtccgg caagcaaaag agagggtagt
     1321 gcacgtgacc tcatgggtgc gctccttctc ttttgtgctt tggttatgac gtctcattga
     1381 ccggctcgca ggattgagcc gcaacaaagc tcgtcgtttg tgaaatgcga gacccggctg
     1441 gtctctcttg tctgtctgct ctgtctagtt atccgattct ccaaaccaga tagaccggaa
     1501 agaccgaaaa gaccagacag acccatctcc gccctgctct gtatctgagt gagagaagaa
     1561 agcaggttct catgaaacag tatcgtgtga agataggccg caagttatct ctgaagcagc
     1621 acgacccaga cggtaccggc aactacaaga aagacgacga cggcagagca tctgcaaagg
     1681 ccgagacgga taagcacatt gccacaatca gtcagctgca agagcgtctc tatgccaaca
     1741 gcgcacgggc gttattgatc gtccttcagg gcatggatac cagcgggaaa gatggcacga
     1801 tccgcagtgt gatgtccggc gtgaatcctc agggctgtaa ggttgtgtcg ttcaaggcgc
     1861 cttcctccca agaactggcc catgactttc tctggcgcgt acatagggaa gtcccggcga
     1921 aagggcagat aggggtgttc aaccggtcgc attatgagga tgtgctgatc actcgcgtgc
     1981 atggttgggt atcagacgag gtggtggagc gtcggttcga gcagatcaag gagtttgagg
     2041 aactgcttac ggaaaatggg acgactattc tcaaattttt tctccacatc tcgaagaagg
     2101 aacagaagga gcgcttggag gaacgcgtgc gcgatccaga aaaacgctgg aaatttaacg
     2161 aaggggatct tgaagaacgg agactgtgga acaggtacat ggttgcgttc gaagatatga
     2221 tggctgccac aagcaccgct catgctccct ggtatatcgt gcctgctaac cggaaatggt
     2281 atcgaaacct tgtggtcgcc ggtcgagtcg tagaggcgat ggaggacatg aaactgaaaa
     2341 caccgccgcc ctccccagga gtcaacttcg atacgttgaa gattgtctag gagcctggac
     2401 gagccgtttc ttgacacatt gggcagggct atattagcat gcgcctcc
//
LOCUS       DATMXI010000259         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_17929, whole genome shotgun sequence.
ACCESSION   DATMXI010000259 DATMXI010000000
VERSION     DATMXI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17929"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            469..2295
                     /gene="infB"
                     /locus_tag="VN444_04930"
     CDS             469..2295
                     /gene="infB"
                     /locus_tag="VN444_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWR21188.1"
                     /translation="MVTVIPPVPQPQVQAEQAEREPLPPLRPIVKIAETITVKELAEK
                     IAMSPSEIIKQLIKMGIMTTINQPLDVEVVKGAADKLGFSVEVTPLEETAAGAKELED
                     LSLLLPRPAVVTIMGHVDHGKTSLLDAIRQTNVIASEAGGITQHIGAYQVDLPGGKIT
                     FLDTPGHEAFTAMRARGAQATDIVVLVVAADDGVMPQTVEAINHAKDAGVPILVAINK
                     IDKPGADSNRARQQMAEYGLVPEDWGGQTVYVEVSAKKKEGIDHLLEMLLLLAEVAEL
                     RANPHRAAKGVIIEAELDRSRGPVATVLVQNGTLNVGDVIVAGLHSGRVRAMNNETGK
                     KVQMAGPATPVEVLGLSGVPMAGDTFVVVSDERKGRLIAISRQQKHREERIGSKHRMT
                     LEDLHRRIREGEVKELRMIIKGDFQGSVGPLRESLERISTEAVRLKVIHASVGAINET
                     DVMLASASNAVIVGFNVRPEPKTQKLAELEGVEIRLYTVIYNAINEIRQAMEGLLEPK
                     YVERSIGRVEVRQVYTVPKVGAIAGSYVVEGKVCRDSQVRLIRDGKVIHKGRVGSLRR
                     FKEDAREVQSGFECGVGLMNFNDVRLGDILEVFDLESVAQKL"
     gene            2387..2674
                     /locus_tag="VN444_04935"
     CDS             2387..2674
                     /locus_tag="VN444_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HWR21189.1"
                     /translation="MTVGTCRVELRLAGNDSLKGKRRVVKSLKDRIRGRFNVSVAEVD
                     RLDEWQRATLGIVCVSNSSRLVDETLAKVVNLIEADADAVMLDYEIELMAH"
     gene            2709..3071
                     /gene="rbfA"
                     /locus_tag="VN444_04940"
     CDS             2709..3071
                     /gene="rbfA"
                     /locus_tag="VN444_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWR21190.1"
                     /translation="MQGRRADRVSTLIQEEISRLILQSVKDPRIGFVAVTRVSVTDDL
                     RQAMVYIASIKGEEKQRQESLIGLKRAAGFLRGELGRRLTLRYVPEILFSLDDSLEQE
                     LHMDKLFRQIEATETKEQ"
     gene            3074..>3767
                     /locus_tag="VN444_04945"
     CDS             3074..>3767
                     /locus_tag="VN444_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWR21191.1"
                     /translation="MASRVDLHLHTKASDGALRPEELVKAADMIGIRVMAVTDHDSVE
                     GIAEAQAAASGLAIEVIPGIEISTNLDGDELHILGYLLNAGDPFLQKALRRLQDDRLA
                     QAKAMVERLGALGCPLEWDRVMAIADGGSVGRPHIAMALVERGYVASVDEAFSRFLRR
                     GGPGYVEGPKLLPQEAVSLIRAAHGVPSLAHPIIVGASDYHLDLPRLLPMMMDAGLEG
                     IEVYYKGYTSEIT"
BASE COUNT          829 a          987 c         1217 g          734 t
ORIGIN      
        1 gatgaggacc atgtaaggtc gcttctggca gccgtaccgc ctcagaagaa aagccgtccc
       61 aagcctaaac cgctggaaac ggttccttcg gctccggagg tccacgccac gcccgtggaa
      121 agccgacggc ccaaggcagg aaaggccgac gtgtcgacaa caacgaccaa gctctcatct
      181 gacaaaccca ctcgatccga gcttatcgag acaaaggcgc cgtcaaagat caagccaata
      241 agcgaagggg cgagacccgt tgcgagagtg agctcgtttg aacaaaaggg acctcagaag
      301 cccaatgcag tctctgtccc accatcacct gagaggacag cgattctcga acggccaccc
      361 tctgtgtcgt tagagcctcc tcacaaggct ccggtcgcga cagcagacgt gaaaaaggag
      421 ccatcccgga ctccgttgcc acctctatca ccatccgtcc acgggcagat ggtgaccgtg
      481 atcccgccag tccctcaacc tcaggtccag gcagagcagg ccgagcgcga gccccttcct
      541 ccgctacgcc cgatagtcaa gattgcggag acgatcaccg tgaaggagct agcggagaag
      601 atcgcgatga gccctagcga gatcatcaag cagttgatca agatggggat catgaccact
      661 attaaccagc cgctcgatgt ggaagtggtc aaaggtgcgg ccgacaagct agggttctca
      721 gtggaagtga cgccgctgga agagactgcg gccggggcga aggagcttga ggacctgtcg
      781 ttgctcctgc ccaggcctgc ggtggtcacg atcatgggtc atgtcgatca cggcaaaacc
      841 tccctgctgg atgcgatccg gcagaccaat gtcatcgcct cggaggcagg cgggattacc
      901 caacacatcg gcgcctatca agtcgatctg ccgggtggca agatcacgtt cctggacacg
      961 ccgggccacg aggcgtttac cgccatgcgg gcgcgtgggg cgcaggcgac cgatatcgtg
     1021 gtcttagtgg tggcggccga tgacggggtg atgccccaga ccgtggaggc gattaatcac
     1081 gccaaggacg ctggcgtccc gatcctggtc gcgattaaca agatcgataa gcctggcgcc
     1141 gattcgaacc gtgccaggca acagatggcg gagtacggtc tggtcccgga agattgggga
     1201 gggcagacgg tctatgtgga ggtctcggcc aagaagaagg agggtataga tcaccttctc
     1261 gaaatgctgc ttctgctggc cgaggttgcc gaactgagag cgaacccgca cagggcagcg
     1321 aagggtgtca tcatcgaggc cgaactggat cgtagtcggg gaccagtcgc gacagtgttg
     1381 gtgcagaatg ggaccctgaa tgtgggggac gtgatcgtcg ccggattgca ttcgggtcga
     1441 gtgcgggcta tgaacaatga gacggggaaa aaggttcaga tggccgggcc cgcgaccccg
     1501 gtggaggtgc tgggtctttc gggtgtcccc atggccggag atacgtttgt tgtggtatcc
     1561 gacgagcgga agggacggct gatcgccatc agccggcagc agaaacatcg cgaggagagg
     1621 atcggctcga agcaccgtat gaccctggag gatttgcacc gtcgcatccg ggagggtgaa
     1681 gtcaaagaac ttcggatgat tattaagggg gacttccagg gctccgtcgg gccgcttcgc
     1741 gaatcgctgg agcggattag cactgaggcg gtcaggttga aggtgatcca tgcgtcggtc
     1801 ggcgccatca acgaaactga cgtgatgctg gcgtcagcct ccaacgctgt cattgtagga
     1861 ttcaacgtgc gtcctgagcc aaagacccag aagctggccg agctggaagg tgttgagatt
     1921 cggctctaca ccgtcatcta caatgccatc aacgagatca ggcaggcgat ggaggggctg
     1981 ctggagccca aatatgtcga gcggtcaatc ggtcgcgtag aggttcgtca ggtctatact
     2041 gtgcccaaag tgggcgccat tgccggctca tacgttgtgg agggtaaggt ctgccgggat
     2101 agtcaggtcc gtcttattcg ggatggcaag gtgatccaca aggggcgagt tgggtcactt
     2161 cgccgcttca aggaggatgc ccgagaggtg cagagcgggt ttgagtgcgg tgtcggcctg
     2221 atgaacttca acgacgtcag acttggcgat atcctggaag ttttcgacct tgaatcggta
     2281 gcccagaaac tctaataaag atgtccaagg ttcaaagttc aacgtgcaag gttcaagggt
     2341 gtaagaatct gaaactttga actttgaacg ttgaactgtg ttgaccatga ccgttggaac
     2401 gtgccgcgtc gagttgcgtc tagctggcaa cgactccctc aagggtaagc gacgggttgt
     2461 caagagcctc aaagatcgta tccgaggccg cttcaatgtc tctgtcgctg aggtggatcg
     2521 tcttgatgaa tggcaacgcg ccacgcttgg gatcgtctgc gtcagtaaca gttctcgatt
     2581 agtagacgag actctagcca aagtggtgaa ccttatcgag gctgacgccg acgccgtaat
     2641 gctcgattac gagatcgagc tgatggctca ttgatgctac cgggctcttg gctgcggaga
     2701 ggatggtcat gcaaggtagg cgcgctgacc gcgtgagtac cctgatacag gaggagatca
     2761 gccgtctgat cttgcagtcg gtcaaggacc cgagaattgg ttttgtcgcg gtcacccgtg
     2821 tcagtgtgac cgatgactta cgacaggcga tggtctatat tgcctcgatc aaaggtgagg
     2881 agaagcagcg ccaggagtcg ctgatcggcc taaagcgtgc ggctggcttt ctccgtggag
     2941 agctagggcg gaggcttact cttcgctatg tcccggaaat tctcttctcg cttgatgatt
     3001 cgctggagca ggagttgcac atggataagc tgttccggca gatcgaggct accgagacta
     3061 aggagcaatg acgatggcga gtcgtgtcga tctacacctt cacacgaagg cctccgacgg
     3121 tgcccttcgg ccggaagagc tggtgaaggc cgccgacatg attgggatcc gcgtcatggc
     3181 tgtaaccgac cacgatagcg tagaggggat cgccgaggcg caagcggccg catcaggcct
     3241 ggcaatcgag gtaattccgg ggatcgagat cagcacgaac ctggacggag acgagctcca
     3301 tatcctgggt tacctgctca acgcgggcga cccgttcctg caaaaggccc tccgtcgatt
     3361 gcaggacgat agactcgctc aagccaaagc catggtcgag cggttaggtg cgctcggatg
     3421 tccgctcgag tgggaccgcg tgatggccat agcggatggg ggatcggttg gacggccaca
     3481 tattgcgatg gcccttgtag agcgtggcta cgtcgcgtcg gtggatgagg cgttctcgcg
     3541 ttttctaagg cgcgggggcc ctggctatgt tgagggtccg aaactccttc cacaggaggc
     3601 ggtcagcctg atcagggcgg cccacggtgt gccctcgctg gcgcatccga tcatcgtggg
     3661 ggcaagcgat taccacctgg atctgccgcg actgctgccg atgatgatgg atgctggctt
     3721 ggaagggatc gaggtctact acaaagggta tacctcggaa attacag
//
LOCUS       DATMXI010000260         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_235803, whole genome shotgun sequence.
ACCESSION   DATMXI010000260 DATMXI010000000
VERSION     DATMXI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235803"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN444_04950"
     CDS             complement(<1..255)
                     /locus_tag="VN444_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HWR21192.1"
                     /translation="MAQGTSIEWTDATWNPVTGCTKISPGCQHCYAERLSHRLQAMGN
                     YNYRNDFEVTLQEQILRLPFSWKRPHRVFVNSMSDLFHEEV"
     gene            complement(321..803)
                     /locus_tag="VN444_04955"
     CDS             complement(321..803)
                     /locus_tag="VN444_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HWR21193.1"
                     /translation="MWSQRHQRRGNARSRTVKLYTIGFTKTTAESFFSRLSKAGVKKV
                     VDVRLNNVSQLAGFAKRNDLQYFLKAICEIDYEHRPELAPTQDILDAYKKHRGDWAIY
                     EQQFLELMIRRKIEDTIPRSAIDEACLLCSENQPHHCHRRVVAEYLKERWGDLDIVHL
                     "
     gene            complement(973..1566)
                     /gene="lexA"
                     /locus_tag="VN444_04960"
     CDS             complement(973..1566)
                     /gene="lexA"
                     /locus_tag="VN444_04960"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWR21194.1"
                     /translation="MVLTDRQRQILNFIATYKARHSAPPTQREIARHVKIYIRGVQYH
                     LERLEKAGYLTRTPKRARAINLRREQRAALTPLLGRVAAGRPVLAEEHIETSYPLPQE
                     WTGSGKTFLLKVHGDSMRDARIFDGDLVLVTVQPEAHPGEIVVAMVEDEVTVKRFQLD
                     GTAIVLQPENEQFAPIRIENGERVRILGKVIGVFRKL"
     gene            1949..2161
                     /locus_tag="VN444_04965"
     CDS             1949..2161
                     /locus_tag="VN444_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HWR21195.1"
                     /translation="MSDPLHGITLQAILERLVEHYGWEALGRRIRIRCFMSDPSIKSS
                     LTFLRKTPWARLKVENLYLALIGEAE"
     gene            2158..>2407
                     /locus_tag="VN444_04970"
     CDS             2158..>2407
                     /locus_tag="VN444_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HWR21196.1"
                     /translation="MIQIYVDSDACPVKAEVIRVAKRYGLDVTFVSNFWMRLPEEWGA
                     QLVVVGGQFDAADDWIVEHVMKKDVVVTGDIPLAHRCIK"
BASE COUNT          479 a          674 c          681 g          573 t
ORIGIN      
        1 cacctcttca tgaaagagat cgctcattga attgacgaag acccgatggg gccgcttcca
       61 actgaatgga agccggagta tctgttcctg aagcgtcacc tcgaagtcgt ttcggtagtt
      121 gtagttgccc atggcctgca aacgatggga cagccgctcc gcgtagcagt gctggcaccc
      181 tgggctgatt ttcgtgcaac ccgtgacagg attccaggtc gcatcggtcc attcaatgct
      241 cgtaccttgg gccatagccg atctcctctg acagactcat gtctcaggta tcagccgcac
      301 gcgtcgttgg acctgagcag ctacaggtga acgatatcga ggtcgcccca tctctctttc
      361 aagtactccg cgacgacgcg acgatgacag tgatgcggct gattttcact acacaggagg
      421 catgcctcgt cgattgccga acgcgggatg gtatcctcga tcttacgtct gatcatcaac
      481 tcaaggaact gttgttcata gattgcccag tcgccgcgat gcttcttgta cgcatcaagg
      541 atgtcctgag tcggcgccag ctccggcctg tgttcgtaat cgatctcaca aatcgccttc
      601 aggaagtact gcaggtcgtt cctcttcgcg aagccagcga gctgggagac gttgttcagg
      661 cgcacatcga cgaccttctt gacacccgcc ttcgagagac gtgaaaagaa ggactctgct
      721 gttgtcttcg taaacccgat tgtatacagc ttcaccgtcc gactcctcgc attgcctcgt
      781 ctctgatgac gctgactcca cataggcgat ctgttgcccg cacgatgagg gaggcggcca
      841 cccatgccgc atggctgcgg aggttttggc aacaaagcat cggcgccggc agccttgtta
      901 ttgagaccgg cgataccccc acatctcgaa gacggtcgag cattttaatg gcgcatcacg
      961 tcgtatgcta tcttacagct tccgaaagac cccgatgacc ttccccagaa ttctgacacg
     1021 ctctccgttc tcgatcctga tgggcgcgaa ctgctcattc tccggttgga gaacaattgc
     1081 cgtgccgtct agttggaagc gcttgaccgt gacttcgtcc tcgaccatgg cgacaacgat
     1141 ctcgccaggg tgggcctccg gctgtaccgt cacgagcacg aggtctccgt cgaagatccg
     1201 agcatcgcgc atgctgtcgc catgaacctt taagaggaaa gtcttcccgc ttccggtcca
     1261 ttcctggggg agcggatagc tcgtctcgat gtgctcttct gccaggaccg gtcgaccggc
     1321 cgccacccgg ccgagcaggg gggtgagggc agcgcgttgc tcccggcgca ggttgatggc
     1381 ccgtgcccgc ttgggcgtcc gggtcaggta cccagccttc tccagtcgct cgagatgata
     1441 ctggaccccg cgaatgtaga tcttcacgtg cctggcgatc tcgcgctgcg tcgggggggc
     1501 actatgccgg gccttatagg tggcgatgaa gttcaggatt tgccgctgcc ggtcggtgag
     1561 taccatcggg tctccacggt tgttctcttc gcaactgctt actctttagt attatacgta
     1621 tgactactac gtcaagaaaa aaatggcatc gcgctctccg ggttgcaacg gacggcggcg
     1681 atgggcggac gcgttgagat tactgaagtg gagcagggag tggtcctgat tcgtagttgc
     1741 gcggggtctt gtgtcgatcg gctgttgtca gttatactcc accggtgttg cgccacagaa
     1801 gaatggctcg caatagctca attgctgata tagatgtgct accacagagg gtttagtggt
     1861 tcctgtgctt tcccctccat ccccatgtat cctctgtttg ccctaccgaa tcgaagagag
     1921 gacattaggc acttgaaaac atattccaat gagtgatcct ctacacggca taacgctcca
     1981 ggctattctc gagcgtcttg ttgaacatta cggctgggaa gcattaggac ggcggatacg
     2041 catccgttgt tttatgtccg atccgagtat caagtcgagc cttacgtttc tccggaagac
     2101 tccatgggct cgccttaaag tagaaaactt gtatctcgcg ctgatcggag aggccgagtg
     2161 attcagatct atgtcgactc cgatgcctgc ccggtcaagg cggaggtgat ccgcgtggct
     2221 aaacggtacg ggctcgacgt gacgttcgta tccaatttct ggatgcgcct tcccgaagag
     2281 tggggcgcac agcttgtcgt agtcgggggg cagttcgacg cagcagacga ctggatcgtc
     2341 gagcatgtta tgaagaagga tgttgtcgtg acgggggaca ttcctctcgc tcaccgctgt
     2401 atcaagg
//
LOCUS       DATMXI010000261         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_285771, whole genome shotgun sequence.
ACCESSION   DATMXI010000261 DATMXI010000000
VERSION     DATMXI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285771"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..337
                     /locus_tag="VN444_04975"
     CDS             44..337
                     /locus_tag="VN444_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21197.1"
                     /translation="MGSGLGCQPDRHVSNYYPDFLVKLSNKRIVIVETKGQEELDVPL
                     KMERLRQWCEDINRVQSDVTYDFVYVDEESFEKYKPTLFRQLVEGFREYKGKI"
     gene            460..876
                     /locus_tag="VN444_04980"
     CDS             460..876
                     /locus_tag="VN444_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWR21198.1"
                     /translation="MKRMGLLLTLTLIVGIALGVIGTQALNAQQEPMKRTVLLKTDLA
                     GIEGKEGVLALVEFAPGAATQSHYHPGEEMAYLLEGTVSLEVQGKSPITFKPGDTFHQ
                     PPKQVHRVKNLSSTMPAKALAFTIAGKDQPLTVPVK"
     gene            1044..1646
                     /locus_tag="VN444_04985"
     CDS             1044..1646
                     /locus_tag="VN444_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWR21199.1"
                     /translation="MAYEARNYEQLLGIEGLSDPLMKNHFTLYQGYVANTNKLADALC
                     QMLKDGKTAAPEYAELKRRFGWEFNGMRLHEYYFGNMVKGGRGLDPASELCQKLAADF
                     GSYDNWQKDFKGSGAMRGIGWVVLYLDPVANRMCNAWIGEHDTGHLAGGVPLLIMDVF
                     EHAFMIDYGLKRADYIEAFFKGIDWSAVTSRYANAPKVTL"
     gene            1663..1866
                     /gene="iscX"
                     /locus_tag="VN444_04990"
     CDS             1663..1866
                     /gene="iscX"
                     /locus_tag="VN444_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HWR21200.1"
                     /translation="MSLYWDDTYEIAQALIQSHPEQDPLEVPFTTLHKWITELADFDD
                     NPNGASEARLEAVQMAWYEEATG"
     gene            1892..>3132
                     /locus_tag="VN444_04995"
     CDS             1892..>3132
                     /locus_tag="VN444_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWR21201.1"
                     /translation="MPVRSYDTLQGTLERITYVNEENHYVVARLQVSGRRDLATIVGN
                     LPTVTPGETLKLTGEWVQHNRYGEQFKIETFETITPATVAGIEKYLGSGLIKGIGPIF
                     AGRLVEIFGIDTLRIIEEEPSRLLTVDGIGEVRLQRIRTAWEEQKEIREVMIFLQGHG
                     VSSAYAAKIFKTYGKSSIAIVQENPYRLAKDIYGIGFKTADRIAQAIGIEPHSPLRVE
                     AGVIHVLNELAGEGHVYYPLDGLTKASAGILEVDEDLTTQAVERLRHEERVICEPGPQ
                     GVAVYLASLYAAEEGVARRLHALAEGGALPADIDIERAILWSEQVNRLSLAEQQKEAI
                     REALIRKLLVITGGPGTGKTTILRCILQILEKKHRRMLLCSPTGRAAKRMSEATGRAA
                     KTIHRLLEFSPKDGRFKRDQH"
BASE COUNT          722 a          835 c          978 g          597 t
ORIGIN      
        1 gcacagcgga gagtggacgc acccgagaca gtagaagccc cctatgggta gtggtcttgg
       61 gtgtcaaccg gataggcatg tctccaacta ctacccggat tttctggtca agctatccaa
      121 caagcgaatt gtcattgtcg agaccaaagg ccaggaagag ctggacgtgc cgctaaagat
      181 ggagcggttg cggcaatggt gcgaggacat caaccgggtg cagtcggatg tgacctatga
      241 ctttgtgtac gtggacgaag agagctttga gaaatacaag cccaccttat tccggcaatt
      301 ggttgagggc ttcagggaat acaaagggaa aatataaagg atttttgccg aattgtcggg
      361 ttacgctgcg ctaacccgac ctacgaccta cggtctgata aggctcaatt gacgcaatag
      421 acacgacaga cgctcttctg gggaattaag gagggaatta tgaagcgaat gggtttactg
      481 cttaccttga ccttgattgt tgggatcgcg ctgggggtga tcgggactca ggctttgaat
      541 gcgcaacagg agccgatgaa gcgaaccgta ctcctcaaga cagatctggc agggattgag
      601 gggaaagaag gtgtgctggc cttggtggaa ttcgcgcctg gagcggcgac tcagtcccac
      661 tatcaccctg gggaggaaat ggcgtatctt cttgaaggca cggtcagcct ggaggtccag
      721 gggaaatcgc ccatcacgtt caagcccggc gacacgtttc atcagccgcc gaaacaggtg
      781 catcgcgtga agaacctcag ctcgaccatg ccggccaagg ccttggcgtt caccatcgca
      841 gggaaggacc agccactcac cgtccccgtg aagtagtctt atattgccca gaaagtcccc
      901 cccatcctca ccttccccct cgtgggggga agggacacct cacgcgaact cctcccccct
      961 tgagggggag gatacaggtg ggggggtgat gggtccggaa acacgggtca acccttatcg
     1021 gttcgtcaga ggaggaacaa ccaatggctt acgaggcacg gaactacgaa cagttgctgg
     1081 gaatcgaagg attgagcgat ccgctcatga aaaatcattt tacgctctac caggggtatg
     1141 tcgctaatac caataaactg gccgacgcgt tgtgtcagat gctgaaagac gggaagaccg
     1201 ccgcgccgga atacgcggag ttgaagcggc gattcggctg ggaattcaac ggaatgcgct
     1261 tgcatgagta ctattttggc aatatggtga aaggcggaag gggcctggat ccggcctcag
     1321 agctctgtca gaaacttgca gcagacttcg gctcatacga caactggcag aaggacttca
     1381 agggatcggg agccatgagg gggatcggtt gggtggtctt atacctggac ccggtcgcca
     1441 atcggatgtg caatgcctgg atcggtgaac atgacaccgg gcacctggcc ggaggcgtcc
     1501 cgctgctgat tatggatgtg tttgaacatg cctttatgat cgactacggg ctcaagcggg
     1561 ctgattacat cgaagccttc ttcaagggca ttgactggtc ggctgtgaca tcgcggtacg
     1621 ccaacgctcc gaaggtaacc ttatagaggt aggctgagta agatgagcct gtattgggac
     1681 gacacgtacg agattgcgca ggcattgatt cagtctcatc ctgaacagga tcccctggag
     1741 gtgccgttta ccactctgca caagtggatc accgagctcg ctgatttcga cgacaacccc
     1801 aacggcgcct ctgaggcgag gttagaggca gtccagatgg cctggtacga ggaggcgacg
     1861 ggctaaaccc tcacgtcgga gtggcgccct gatgcctgtg cggtcatacg acacgctcca
     1921 ggggacgctg gagcggatca cctatgtcaa tgaagagaac cactatgtgg tggccaggct
     1981 ccaggtgtct gggcggcgcg acttggccac cattgtgggc aacctgccaa ccgtcacgcc
     2041 aggcgagacc ctcaagctga ccggagagtg ggtccagcac aacaggtatg gcgaacagtt
     2101 taagatcgag acgttcgaga ccatcacccc cgccacggta gccggaatcg agaaatacct
     2161 gggctccggc ctcatcaaag ggatcggccc gatcttcgcc ggaaggttgg tggagatctt
     2221 tggcatcgac accctgcgca tcatcgagga ggagccctcc cgcctgctca cggtggatgg
     2281 gatcggcgag gtacgacttc agcgaatccg gaccgcctgg gaagagcaga aagagatccg
     2341 ggaggtcatg atcttcttgc agggtcacgg cgtctcctcc gcctatgccg ccaagatctt
     2401 caagacctac ggaaaatcct cgatcgccat cgtccaggag aacccctacc ggctggccaa
     2461 ggatatttac ggaattggat tcaagaccgc ggaccgaatc gcccaggcga ttgggatcga
     2521 gccgcattcg ccgctgcggg tagaggctgg ggtgatccat gtcttaaacg agctggccgg
     2581 ggagggccac gtttactacc cccttgacgg cctcacgaag gcgagcgccg gtattttaga
     2641 ggtggacgag gatctgacga cccaggcggt tgagaggctt cgtcacgagg aacgggtcat
     2701 ctgtgagcct gggccgcaag gggtggcggt ctatctcgcc tcgctgtacg cagcggagga
     2761 aggagttgcc cggcgcctgc atgccctggc tgaaggcggc gcgctccctg cggacatcga
     2821 catcgaacgc gccatcctct ggtcggagca ggtgaatagg ctgagcctgg cggagcaaca
     2881 gaaggaggcg attcgtgagg ccctcatacg taagctgctg gtcatcaccg gcggtcccgg
     2941 caccggcaag acgaccatcc tgagatgcat cctgcagatc ctggagaaga aacatcgtcg
     3001 aatgctcctc tgctctccta cggggcgggc ggcaaagcgg atgagtgagg ccacgggacg
     3061 agcggccaaa accatccacc gcctcctgga gttcagcccc aaggatggtc ggttcaagcg
     3121 ggatcaacac ag
//
LOCUS       DATMXI010000262         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_135304, whole genome shotgun sequence.
ACCESSION   DATMXI010000262 DATMXI010000000
VERSION     DATMXI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135304"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /gene="lnt"
                     /locus_tag="VN444_05000"
     CDS             <1..829
                     /gene="lnt"
                     /locus_tag="VN444_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWR21202.1"
                     /translation="YRRLSVEATKYVPALIVWPETAVPFFLQYEPALQGRVLDIAAET
                     GSYLLVGSPDAEPSAAANGGTRYHNSAFLISPKRELLSRYDKIHMVPFGEYVPLKSIL
                     FFVHKLAYGIGDFEGGRTYTIFDIPGGRFGATICYEAIFPDQVRRYVKEGAEFLVNIT
                     NDAWFGRSAAPTQHLAMAVLRAAENRRYLIRAANTGISAIVDSSGRILQASDIFVPTV
                     ITEQIRAERTHTFYTRYGDLFAWVCVIFIIAVFMARWTWSAVRIAPVPAAASQRRTQ"
     gene            826..1942
                     /gene="prfB"
                     /locus_tag="VN444_05005"
     CDS             join(826..894,896..1942)
                     /gene="prfB"
                     /locus_tag="VN444_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107563908.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWR21203.1"
                     /translation="MIPEFARTLDELKEKLHTLRVYLDVAGKQLRLAAIEELLHSQEF
                     WADTSSAREIMKEQRALKDVVDQPEAIGRELEDLTILLGLLREEEDPQVLKELESSLR
                     NLESQVVALELMTVMTGEYDQGNAILTINPGAGGTESQDWAQMLLRMYLRWAEAGRYK
                     TEVIDLLPAEEAGIKTATVTVTGKYAYGRLKAEIGVHRLVRISPFDANHRRHTSFASI
                     FVYPEIDETIDVSIDEKDLRIDTYRSSGAGGQHVNVTDSAVRITHLPTKIVVSCQNER
                     SQHKNKAMAMKVLRARLYDHYRREQEKEMAKLEGEKKDIAWGSQIRSYVLAPYQLVKD
                     HRTGLETGNVEKVLDGEVEPFIDAFLLKGRSVSEAAK"
BASE COUNT          446 a          549 c          610 g          427 t
ORIGIN      
        1 atatcgccgt ctcagtgtgg aggcgacaaa gtacgtgccg gccctcatcg tgtggcctga
       61 aactgccgtt cccttctttc ttcagtatga accggcgttg caagggcgcg tcctggatat
      121 cgctgctgaa accggcagct atctgctggt gggcagtccg gatgccgagc cgtccgcggc
      181 cgccaatggc gggacccgct atcataatag cgcgttcctg atctctccca agcgggaact
      241 cctcagccgg tacgacaaaa ttcacatggt tccttttggg gagtatgtcc ccctcaaatc
      301 gatcctattt tttgtccata agctggcata tggaatcggc gacttcgagg ggggccggac
      361 ctacacgatc tttgatatcc cgggcggccg tttcggagcc accatctgct atgaggcgat
      421 ctttccggat caggttcgac gctacgtgaa agaaggggcg gagttcctgg tcaatatcac
      481 gaacgatgcg tggttcggcc ggtcggctgc cccgactcag catctggcaa tggcggtcct
      541 tcgggctgcc gagaaccgcc gctacctgat ccgagcggcc aataccggca tttccgctat
      601 tgtggattcg agcggccgga tcctccaggc ctccgatatc tttgtaccca ccgtgattac
      661 agaacagatt cgcgcagagc gaacgcacac gttttacacc cgttatggtg atctcttcgc
      721 gtgggtctgt gtcatcttta ttattgcagt attcatggca aggtggacct ggagtgccgt
      781 tcggatcgct ccggtgccgg ctgccgcttc acaaaggagg acgcaatgat cccagagttc
      841 gcgcgcacac tcgatgagtt gaaggagaag ctgcataccc tgcgggtgta tctttgacgt
      901 cgcgggaaag cagttacgcc tggccgcgat tgaagagctg ctccattcac aggaattctg
      961 ggccgacacc tccagtgctc gggagatcat gaaggagcaa cgcgcgctga aggatgtggt
     1021 tgaccaaccg gaggccatcg gacgcgaact ggaggatctg acgatcttgc tcgggctact
     1081 gcgggaagaa gaagacccac aggttctgaa agagttggag agttcgcttc ggaacctgga
     1141 gagtcaggtt gttgcgctcg agctgatgac ggtcatgacg ggagaatacg accagggtaa
     1201 cgcgatcctt accatcaatc caggcgccgg cggtaccgag tcgcaggatt gggcacagat
     1261 gctgctgcgg atgtacctgc gttgggcgga agccggtcgc tataagaccg aggtcatcga
     1321 tctgctccct gcggaggagg ccgggatcaa gactgccacg gtgacggtga cgggcaagta
     1381 tgcctatggc cgcctgaaag ccgagattgg ggtccaccgt ctggttcgca tctctccgtt
     1441 cgatgccaac caccgccgtc atacctcctt tgcgtcgatc ttcgtctacc ctgagatcga
     1501 tgagaccatc gatgtatcca tcgacgagaa ggatctgcgt attgacacct accgctccag
     1561 cggggcaggc gggcagcatg tgaacgtcac cgactcggcg gtacggatca cacacctgcc
     1621 gacaaaaatc gtggtctcct gtcagaatga acgctcgcag cataagaata aggccatggc
     1681 catgaaggtc ctgcgagcca ggctgtacga ccactatcgt cgagagcaag agaaggaaat
     1741 ggccaaactc gaaggcgaaa agaaggacat tgcgtggggc agtcagattc gttcgtacgt
     1801 gctcgcgccc taccagctcg tgaaggatca ccggaccggc ctggagacgg gcaatgtcga
     1861 gaaagtactc gacggagagg ttgagccatt catcgatgcc tttctgctga aagggcgaag
     1921 cgtgtctgag gctgcgaaat gagatctgca gtattgaggt aacactcagg taaatagacc
     1981 gaagactgaa ggcttttagg ggtaatcatg cgtggtcaca caacacttcg ag
//
LOCUS       DATMXI010000263         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_12020, whole genome shotgun sequence.
ACCESSION   DATMXI010000263 DATMXI010000000
VERSION     DATMXI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12020"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="VN444_05010"
     CDS             complement(<1..1664)
                     /locus_tag="VN444_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWR21204.1"
                     /translation="MGLRQGKVVAVKGDPEAPVNRGLLCIKGYSLPKILYGPDRYSVP
                     LLRQGNKFVKISWNQALDIMASKFKEALQQHGPESVAVYFSGQTTIFEGYAINKLMKA
                     GIGSNNVEGNPRLCMASAVTGFYSTFGMDEPMGCYDDIELTDTFFVWGSNFAEAHPIL
                     YSRMMERKHQDSNVKIVSLQTFLNRSTDEPADIVAIFKPHSDLALANAMAHVIVKEGL
                     TNETFIQQHVTFKKGLTGIGYGVEENFEYGGAPEASWKTYKPFTDKAQPISFEEYKKF
                     LETYTPEYAEILSGIPADTIRQIARLLGDPKRKAVSCWTMGFNQHVRGTWINNLVYNL
                     HLLTGKIAEPGNGPLSLTEQPSACGTTREVGVLSHALPGGMFVANPEHRKKTAHIWNV
                     PVEKISSRPGFHTMEMFRALDRGEIKVIWINTTNPFQTLPHVGRYRRGARKGGERFVV
                     VSDVYPSETARHADLILPSAMWVEKEGMFGNTERRSQHWFKMVDPPGEAKDDLWQIVE
                     LAKRLDLGHLFDYGEVPLQKALFEEYRKFGEGSGKDLAPYDVYAKVR"
     gene            complement(1891..>2091)
                     /locus_tag="VN444_05015"
     CDS             complement(1891..>2091)
                     /locus_tag="VN444_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HWR21205.1"
                     /translation="VIKTLNPGPGATHPQFTPKGEAAYVSLMDGGKVVVYDTATFKVL
                     KEFPADHPSGIFFTNRAHKFGM"
BASE COUNT          467 a          600 c          557 g          467 t
ORIGIN      
        1 cggaccttcg cgtacacgtc ataaggcgcg agatcttttc ccgacccctc gccgaatttt
       61 cgatactcct caaagagcgc cttttgcaga ggcacctcgc cgtaatcgaa caggtgtccc
      121 aaatcaagac gtttggctaa ctcgacgatc tgccagagat cgtccttggc ttcgccaggc
      181 gggtcgacca tcttgaacca gtgctgtgag cgccgctcag tgttaccgaa catcccctcc
      241 ttctcgaccc acatggcaga cggaaggatg agatcggcgt gtcgagcggt ttcgctggga
      301 tacacatcgg agaccacaac gaaccgctcg cccccctttc gcgcacccct gcgatagcga
      361 ccgacatggg gcaaagtctg aaaggggttg gtggtgttga tccagatcac cttgatctct
      421 ccgcgatcca gtgcgcgaaa catctccatg gtatgaaacc ctggtctgga tgaaatcttc
      481 tcaaccggaa cgttccaaat gtgcgcggtt tttttccgat gctccggatt cgcgacaaac
      541 atgccgccgg gaagagcgtg ggatagtacg cctacctccc gcgtagtccc gcacgctgaa
      601 ggctgctcgg tcagcgataa ggggccgttg ccaggctcgg caatttttcc ggtcagcagg
      661 tggaggttat agacaaggtt gttgatccag gtgccgcgta cgtgctggtt gaagcccatc
      721 gtccaacagg acacggcctt acgcttggga tcgcccaata gtcgagcaat ctgacggatc
      781 gtgtcagccg ggataccgga gagtatttca gcatattccg gtgtataggt ttccaggaat
      841 ttcttgtact cctcgaagct gatcggttga gccttgtcag taaaaggttt gtacgtcttc
      901 caactggctt ccggagcccc gccatactca aagttctcct ctacaccata gccgatccct
      961 gtcagcccct tcttgaaggt gacatgctgc tggataaacg tctcgttggt gagcccctct
     1021 ttcacgatga catgcgccat ggcattcgcg agggcgagat ctgagtgagg cttgaaaata
     1081 gccacgatgt ctgcgggctc atcggtgctg cgattgagaa aggtttgcag gctgacgatc
     1141 ttgacgttgg aatcctggtg cttccgctcc atcatcctcg agtagaggat tggatgcgcc
     1201 tcagcgaagt tagagcccca gacaaaaaag gtatcggtta gctcaatatc gtcgtaacac
     1261 cccatgggct catccatgcc gaaggtcgag tagaaaccag tcacagccga ggccatgcag
     1321 aggcgcggat tgccctcgac gttgttggag ccaatccccg ccttcatcag cttattgatg
     1381 gcgtagcctt caaagatcgt tgtttgaccc gagaagtaca ccgctaccga ctccggacca
     1441 tgctgttgga gagcctcttt aaacttagag gccatgatat ccaacgcctg gttccaagag
     1501 atcttgacaa acttgttacc ctggcgaagc agcggtacac tatagcggtc aggaccataa
     1561 agaattttgg gtaaactata gcctttgatg cagagaaggc ctcgattgac gggagcctcc
     1621 ggatctccct tgacagccac aaccttacct tgtcgaagac ccactagcac gccacatccg
     1681 acaccgcaga agcgacaagg actcttcttc cattcctccg ccgacgcctc ggcagcctcc
     1741 gctagaccct cagaggcagg gagtagtgcg tcaggcgagg agaggagcgc agcacctgct
     1801 ccagccaagg tctttaagag ctctctgcga gaaagtttca taatcgccct ctgctgtccg
     1861 gattaagccg aaaaccggca ccgtaatctc tcacatcccg aacttgtgcg cccgattggt
     1921 aaaaaagatc ccggagggat gatccgccgg gaactccttc agcaccttaa aggtcgccgt
     1981 gtcgtagaca accactttgc cgccgtccat aagagagacg tatgcggctt ctccctttgg
     2041 cgtgaactgc gggtgcgtgg ccccaggccc gggattgagc gttttgataa c
//
LOCUS       DATMXI010000264         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_251686, whole genome shotgun sequence.
ACCESSION   DATMXI010000264 DATMXI010000000
VERSION     DATMXI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251686"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VN444_05020"
     CDS             <1..318
                     /locus_tag="VN444_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWR21206.1"
                     /translation="DQILALLRAQIPEALDAFDPIFGQHAGEKNAALYARCAEILGYD
                     ASEILVIEDSERGFKAANGAGLPCAVVYNEYTRGQDFTGAELVVRNLEHLDLDLLERL
                     CLS"
     gene            complement(815..1321)
                     /locus_tag="VN444_05025"
     CDS             complement(815..1321)
                     /locus_tag="VN444_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWR21207.1"
                     /translation="MTYSANLHGRIHRLAVKSMGSPVPVHIDGKTYEVDFSAVDGSLL
                     SLLVMGRSYEADVVEEAEGVLTVWVQGELYRIEYQEEGRRPRRAASVTGQGMRGRQMI
                     VAPMPGKVVALLVSPEQEVSAGQGVIVIEAMKMENELKASGSGVIKEIKVQEGASVSG
                     GDVLVVIE"
     gene            complement(1318..>2428)
                     /locus_tag="VN444_05030"
     CDS             complement(1318..>2428)
                     /locus_tag="VN444_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWR21208.1"
                     /translation="PGTTRDLTDQEVREAVREFGLPVVIKASAGGGGKGMRIVSTEAM
                     LGSAIRAARSEASSAFGNAAIYVERHLGSARHIEMQIMADTRGNVVYLGERECSLQRR
                     HQKVIEESPSPFMDLELRHRMGEAAVRLARAVDYTNAGTMEFLVDSSKNFYFLEMNTR
                     LQVEHSVTEMVTGLDLVKEQIRIAAGEPLSVRQDEIQLYGHAIECRIYAEDPFNNFMP
                     SPGRIRALRTPGGPGLRIESAIYEGCEVPIYYDPLISKLIVWGRDRGEAIGRMRRALA
                     EYVVLGVKTNIQFHEQVLNLSQFVTGQIHTEFLESWLANGLKSEDGAFAEIALIAGAL
                     YLHTRKRVAPSPIAAGGQVESAWKLAARQDGLRRR"
BASE COUNT          521 a          729 c          638 g          540 t
ORIGIN      
        1 gatcagatcc tcgcgctcct gagggcgcag atccctgagg cgctcgatgc cttcgacccg
       61 attttcggtc agcatgccgg cgagaagaat gcggccctgt atgctcggtg cgccgagata
      121 ctcggatatg atgcgtcgga gatcctggtc attgaggatt ccgagagagg gttcaaggcg
      181 gctaatggag ccggcttacc gtgcgccgtc gtttataatg agtatacacg cggccaggac
      241 ttcaccggcg ccgagcttgt ggtccggaat ctagagcatc ttgacctcga tttgctggaa
      301 aggctatgtc tgagctgacc ataaagggcg caatcgcgtg catgggatcg atccttgaca
      361 tcgccacgaa tgatatgtca ctgtctggca actactcagg caggcaggct gaagactgaa
      421 ggctgttagg ggtaacgtag tgaccacacc atgcttgtcc aagataggat ttttgaaaga
      481 ccggatttgg acagcattct tgaaatctcc cctcgcccct ctttgccaaa gaggggtatt
      541 caccatattt ccccctttga gaaaggggga ttgaggggga tttgcacgtg ggaatatttg
      601 tcttagccat ttggacagca tattgtgata gcggcaacct catttttcag gatgttacag
      661 gtatcaatga aatcacttgc ttcacttgtc caaaaaagat gttttatgaa tattttggac
      721 agcagtgtca gaaaatagct tttaacagtc agccgtcagt ctcagacaca gcggggaacg
      781 tatcagaaga gctgaaagct gatagcatgg gctattactc aatcacaacc aggacatcgc
      841 ctccactgac actggcccct tcttgtacct tgatctcctt gattacacca gaccccgaag
      901 ctttcaactc gttttccatc ttcatcgcct cgatgacaat caccccctga cccgcactca
      961 cctcttgttc tggtgagacg agtaacgcca caacctttcc cggcatcggg gccacaatca
     1021 tctggcggcc tctcatgcct tggccggtaa cgctggcagc tcttcgtgga cgacgcccct
     1081 cctcctggta ctcgattcgg tacagctcgc cctgcaccca gaccgtaagg accccctctg
     1141 cctcttcgac cacatcggcc tcgtacgagc ggcccatcac gagaagcgag agaaggctgc
     1201 cgtcaacagc gctgaaatcg acctcatagg tcttcccgtc aatgtgaacc ggcactggcg
     1261 atcccatact cttcaccgca agcctgtgaa ttctgccatg cagattagcg gagtaggtca
     1321 tcgtcgtcgc agcccgtcct gacgggcggc cagcttccat gcactttcga cctggccgcc
     1381 agccgcgatg ggcgaggggg ctacgcgctt cctggtatgg agatagagcg ctccagcgat
     1441 cagcgcgatc tcagcgaagg ctccgtcctc ggacttcagt ccgttggcca accagctctc
     1501 cagaaattcc gtgtggatct ggcccgtaac gaactgcgaa aggttgagga cctgttcgtg
     1561 gaactggata ttcgtcttca cgccgagcac gacatactca gccagagcgc gtcgcattcg
     1621 tcctatcgcc tcgccccggt cgcgtcccca gacgatcaat tttgaaatca ggggatcata
     1681 gtagattgga acctcgcagc cttcgtagat cgcgctctcg atccgcaggc cgggcccacc
     1741 gggcgtacgc agcgcccgaa ttcgtccggg cgacggcatg aagttattga agggatcctc
     1801 ggcgtagata cgacactcga tcgcatgccc gtacaactga atctcatctt gccgcactga
     1861 gagcggctcc ccagcggcaa tccgaatctg ctctttcacc aggtcaagcc cggtcaccat
     1921 ctcggtgacc gaatgctcta cctgtaaccg cgtgttcatc tccaggaaat agaagttctt
     1981 ggacgaatcg accaagaact ccattgtccc ggcgttcgtg tagtctactg ctctggctaa
     2041 cctcaccgcc gcctctccca tccggtgcct cagttcgagg tccataaagg gtgacggcga
     2101 ctcctcaatg accttctggt gacgccgctg tagtgagcat tcgcgctccc caaggtacac
     2161 cacgttgccc ctggtatcgg ccataatctg catctcgatg tggcgagcgg aacccagatg
     2221 ccgctctaca tagatagccg cgttgccgaa tgcagaggag gcttcagagc gagcagcgcg
     2281 gatggcgcta ccgagcatcg cctcggttga gacgattcgc atccctttcc cgcccccgcc
     2341 agcagaagcc ttgatgacca caggcagacc aaactctcgg acagcctcgc gcacctcctg
     2401 atcggtgagg tctcgggtcg tccctggg
//
LOCUS       DATMXI010000265         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_196568, whole genome shotgun sequence.
ACCESSION   DATMXI010000265 DATMXI010000000
VERSION     DATMXI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196568"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VN444_05035"
     CDS             <1..258
                     /locus_tag="VN444_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWR21209.1"
                     /translation="LAVDQQCGKLVTGPQIICSGFVHAQDSKALAEGIKILVCSVLEN
                     ASEEDRTDLRLMEQRIKTAVKKQLQKEIERRPMILSVIMEV"
     gene            274..2538
                     /locus_tag="VN444_05040"
     CDS             274..2538
                     /locus_tag="VN444_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWR21210.1"
                     /translation="MATEGTPKPIATQREPRGGDRSPVSQLLTHPKTHEALGILGIAV
                     TLFLLASLLSYDSLDPSFFNSGGGPGHQVYNYGGRWGAELAGDLLELLGVSALALPFF
                     LVLLSWRFLRAKATPFVIWKLVGCVLLLLSLGLLAQFFARAGLAVGRIWERPGGFVGE
                     ELHRIFSPLVGRVGLPLLGLTTLVLGMVCLSSRPLAGLSFGFRGVIERVATRMRERRA
                     AKAQLPGRIRPPYTPPAEEEPQIESRSFPVLTEPAPGVATAEPETPAIDPPPQPSFPF
                     AISKEGFQIPPLSLLDLPVGAEGGLSDEERETNAQILERKLLDFGVEGRVTQAQPGPV
                     ITRYEIEPGPGIKINRIVALADDLALALRALSVRVVAPIPGKAVVGVEIPNRRRAMVH
                     LREVLASKEFKGSAAHLPLALGKDIAGEPYVVDLGQMPHLLIAGATGSGKSVCINALI
                     VSLVYKATAESIRLLLIDPKRVELSVYDGIPHLAERVVCDPKEAAKRLQRLVIHMEGR
                     YKLFARLGARNIASYNRLIRIAKREGGGEVFQPLPYLVVVIDELADLMLTASADVERA
                     IARLAQMARAVGIHLIVATQRPSVDVITGIIKANFPARLAFQVSSKVDSRTILDMNGA
                     EQLLGDGDMLFIPPSSSKPHRIHGSFVSDIEIKRVVDFLKAQGKAEEFPWSLLPAEEE
                     QESSGDEDDELYRQAVEIVVTTRQASISLIQRRLRIGFNRAARMIERMEQERIVSRIE
                     GGGPRAVLVEPQNM"
BASE COUNT          516 a          734 c          873 g          545 t
ORIGIN      
        1 cttgccgtag atcagcagtg cggcaagttg gtgacaggac ctcagataat ctgcagcggg
       61 ttcgtccatg cacaggattc gaaggccctc gcggagggca tcaaaatcct ggtctgttcc
      121 gtactggaaa atgcctctga agaagaccgg accgatctgc gcctcatgga gcaacggatc
      181 aagactgcgg tgaagaaaca gttgcagaag gagatcgaac gacggcctat gatcctgtca
      241 gtgatcatgg aggtgtaacg aaggaggcgg ttgatggcga ctgaaggcac gccgaagccg
      301 atagcgaccc aaagggagcc gcgtggagga gatcgatcgc cagtgagcca gctcctcaca
      361 catcccaaga ctcacgaggc gttgggcatc ctcgggatcg ctgtaaccct cttcctcctg
      421 gcaagtctcc tgtcctacga ttccctcgac ccctcgttct tcaactccgg aggcggcccc
      481 ggacatcagg tgtacaatta tggggggcgg tggggggcag agcttgctgg tgatcttctg
      541 gagttattgg gtgtcagcgc gctggcctta ccattcttcc tggtcctgtt gagttggagg
      601 tttctcagag cgaaggctac tccttttgtc atctggaagc tggtcgggtg cgtcctgctc
      661 ctcctgagcc ttggactact ggcccagttc ttcgccaggg caggactggc tgttggccga
      721 atctgggaac gccccggtgg ctttgttggc gaagagctgc atcggatctt cagtccgctg
      781 gtgggccgcg tgggtctgcc gttgctgggg ctcacgaccc tcgtcctcgg tatggtgtgt
      841 ctgagcagtc ggccgcttgc gggtctctcc tttgggttcc ggggtgtgat cgagcgggta
      901 gcgactcgta tgagagaacg gcgagcggcg aaggcgcaac tgccgggcag aatccggccg
      961 ccctataccc ctcccgctga ggaagaacct cagatcgaga gtcggtcgtt tcccgtgctg
     1021 acggagcctg ctcctggggt ggccacagca gagcctgaaa cccccgcaat cgatccccct
     1081 ccccagccat cctttccctt tgcgatttcg aaagagggtt ttcagatccc accgctatcg
     1141 ttgctcgatc tgccggtagg ggcggagggc ggactctccg atgaggagcg ggagacgaac
     1201 gcgcagatcc tcgagcgtaa gctgttggac ttcggggtcg aggggagggt cacgcaggca
     1261 cagccagggc ctgtcattac ccgatatgag attgagccgg ggcccgggat caagatcaac
     1321 cggattgttg ctttggctga cgatctggcc ttggccttac gggcgctcag cgtgcgcgtc
     1381 gtggcgccga ttccagggaa agcggttgtt ggcgtagaga tcccgaaccg ccgtcgtgct
     1441 atggtgcacc tgcgtgaggt gctggcatcc aaagaattta agggatctgc tgcacacctt
     1501 ccccttgcct tagggaagga cattgccggc gaaccctatg tggtcgatct gggccagatg
     1561 ccacacctgc ttatcgccgg ggcgacaggt tccggcaaaa gcgtatgcat caatgccctg
     1621 attgtgagcc tggtgtacaa ggccacagca gaaagcattc gtctgctgct gatcgatccg
     1681 aagcgggtgg aactctccgt ctatgacggg attccgcatc tggctgagcg cgttgtctgc
     1741 gatccgaagg aggcggcgaa acggcttcag cgccttgtga tccacatgga agggcgatac
     1801 aaactgtttg ccaggctcgg ggctagaaat attgccagct acaaccggct gattcggatc
     1861 gcgaagcgag aagggggcgg tgaggtcttt cagccgctcc cctacctggt ggtggtcatc
     1921 gacgaactgg ccgatctgat gctgaccgct tccgccgacg tcgagcgggc catcgccagg
     1981 ctggcgcaga tggctcgggc ggtaggcatc catctgatcg tggcaacgca gcgcccttcg
     2041 gtcgatgtga taaccgggat catcaaggcc aattttccgg cgagactcgc attccaggtc
     2101 tcctcgaagg tcgattcccg gacgatcctc gacatgaatg gcgccgagca actgttaggc
     2161 gatggcgata tgctgttcat cccgccttca agctcaaagc cacaccggat tcacggatcg
     2221 ttcgtatccg atatcgaaat taagcgcgtc gtcgattttc tgaaagcgca ggggaaggcc
     2281 gaggagtttc cgtggtcgct actgccggcc gaggaggagc aggaatcgtc aggggatgaa
     2341 gacgacgagt tgtaccggca ggcggtcgag attgtcgtga ccacgcgtca ggcctcgatc
     2401 tcccttattc agcgacgact caggattggg tttaaccggg cggcgcgaat gatcgagcgg
     2461 atggagcagg aacggattgt gagccgcatt gaagggggcg ggccgcgagc ggtcctcgtg
     2521 gaaccgcaga atatgtgagt gacgtgcgag gtgcccactc cctcgccgac gtaggggcaa
     2581 ccctggcggt cgccagatcc gggcaggcac aaggcctgcc cctacaaggg gaagggggta
     2641 ctgcgtgcga agagaatggg gcggagtg
//
LOCUS       DATMXI010000266         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_114308, whole genome shotgun sequence.
ACCESSION   DATMXI010000266 DATMXI010000000
VERSION     DATMXI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114308"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN444_05045"
     CDS             complement(<1..351)
                     /locus_tag="VN444_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWR21211.1"
                     /translation="MQDKLRFLLVLHNHQPLGNFDEVIQSLLDRAYRPLLEAVYARPA
                     LKFTLHLSGPLLLWLERRAPDYLDLIGELVQRGRLELLSGGLYEPILAAIPHEDRIAQ
                     ITLMSERVRSRFGVR"
     gene            complement(402..>2603)
                     /gene="glgB"
                     /locus_tag="VN444_05050"
     CDS             complement(402..>2603)
                     /gene="glgB"
                     /locus_tag="VN444_05050"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWR21212.1"
                     /translation="IEAIVQGEHGDPFAVLGPHEVRVASEPCVAVRAFLPDAREATVV
                     HADGVSKDQPMELVHPDGLFEVVVPTRDGLFPYRLRVVDRQGQGCEIEDPYRFPPTLS
                     DYDLHLIGEGSHLRNYEKLGAHLITLGGVPGVCFAVWAPNAKRVSVVGDFNHWDGRRH
                     PMRNHPGNGIWDLFIPGLTEDALYKFEIKSQSGEPLALKADPFAFAFEPPPRTASRVF
                     NIDTYRWGDALWMDARSRQNVLERPVSIYEVHLGSWMRVSQEENRFLTYRELAHRLAD
                     YVTEMGYTHVELLPITEHPFYGSWGYQTIGYFAPTCRYGTPTDFMYFVDYLHQRGIGV
                     ILDWVPSHFPRDPHGLAYFDGTHLYEHEDPRKGEHREWGTLTFNYGRKEVDNFLLGNA
                     LFWLERYHLDGLRVDAVASMLYLDYSREPGEWIPNIYGGNENLEAVAFLRRFNELVYA
                     YHSGVMTIAEESTAWPQVSRPTYVGGLGFGLKWNMGWMHDMLGYMALDPIHRSYHHND
                     LTFGLLYAFTENFTLPLSHDEVVHGKGSLLGKMSGDDWQKFANLRCFSVYMYGHPGKK
                     LLFMGGEFGQRREWDHDQNLDWHLLTEGPHHKGLQLLMRNLNHLYRSQPALYEVDFEP
                     RGFEWIDCHDWQGSAVAFLRRAKDQDDFVVVVCNFTPVPRHDYRIGVPTGGYYVELLN
                     SDAAIYGGSNIGNGGGIAAEPIPDHGRPFSLRLTIPPLGGLILKPATGVAL"
BASE COUNT          543 a          762 c          796 g          502 t
ORIGIN      
        1 ccgaacaccg aaacgggatc gaacgcgctc actcatcagc gtgatctggg cgattcgatc
       61 ctcgtgcgga atggcggcca gaatcggctc gtataaccct cctgacagga gctccagccg
      121 gccgcgctgg accagttcgc caatgagatc gaggtaatcc ggggctcggc gttccagcca
      181 cagtagaagc ggaccgctca gatgcagggt gaatttgagc gccggtctgg cgtagacggc
      241 ctcaaggagg ggtcgataag ccctgtccag gagcgactga atcacttcat caaaattacc
      301 aagaggctga tggttgtgca ggacaagcag aaaacggagc ttgtcctgca ctgagcctgc
      361 tctgagtcta gttgaaggag gctgctcagc gggccggtct gtcatagcgc gacacctgtg
      421 gcaggcttaa ggatcaatcc gcccagtgga ggaatagtga ggcgcaatga gaacggtcgc
      481 ccatgatcgg ggatcggctc tgccgcaatg ccgccaccat tcccgatgtt gctgccaccg
      541 tagatcgcgg catctgagtt cagtagctcc acatagtagc ccccggtcgg cacaccgatg
      601 cgatagtcgt gtcggggtac gggggtgaag ttgcagacca cgaccacaaa gtcgtcctgg
      661 tccttcgccc ggcgcaagaa ggcgacggcg ctcccctgcc agtcatggca atcgatccat
      721 tcgaatccgc gaggctcgaa atcgacctcg tagagggccg gttgtgaccg gtagaggtgg
      781 ttcaggttgc gcatcagtaa ctgtaagcct ttgtggtgtg ggccctccgt gaggaggtgc
      841 cagtccaggt tctggtcgtg atcccactcg cgccgctggc cgaactctcc tcccatgaac
      901 aagagcttct tgccaggatg tccatacatg tagacagaga agcagcgcag gttggcgaac
      961 ttctgccagt catcacccga catctttccg agcagtgagc ctttcccatg caccacctca
     1021 tcgtgcgaga ggggcagggt gaagttctcg gtgaaggcgt acagcagacc gaaggtgaga
     1081 tcattgtggt ggtagcttcg atggattggg tcgagcgcca tgtacccgag catgtcgtgc
     1141 atccacccca tgttccactt caatccaaag ccgagcccgc ctacgtaggt cgggcgcgag
     1201 acctggggcc aggccgtgga ctcttcggca atggtcatca cgccagaatg gtacgcatag
     1261 accagctcgt tgaagcgccg aaggaaggcg actgcctcca gattctcgtt accgccatag
     1321 atgttaggga tccactctcc gggttccctg gaatagtcca ggtagagcat cgaggccacc
     1381 gcgtccaccc gcagcccatc caggtgatag cgttcaagcc agaagagcgc attgcctagc
     1441 aggaagttgt ccacttcctt tcggccgtaa ttgaaggtga gcgtacccca ctcgcgatgt
     1501 tcgcccttgc ggggatcttc atgctcatag aggtgggtcc cgtcgaagta ggctaacccg
     1561 tgcgggtcgc gggggaaatg ggacgggacc cagtcaagga tcacgccgat cccgcgctgg
     1621 tgcaggtaat caacgaagta catgaagtcg gttggcgttc cataccggca ggttggcgcg
     1681 aagtagccga ttgtttggta cccccacgag ccgtaaaacg ggtgctccgt gatcgggagt
     1741 agttcgacgt gggtgtaccc catctcggtt acgtaatccg caagccggtg agccagctct
     1801 cgataggtca gaaaacgatt ctcctcctgg gacacccgca tccaggagcc gagatgcacc
     1861 tcgtagatcg ataccggtcg ctcaagcacg ttctggcgag atcgcgcgtc catccataac
     1921 gcatcccccc accggtaggt atcaatgttg aacacacgcg aggcggttcg gggtggtggt
     1981 tcgaaggcaa aggcgaacgg gtcggctttg agggcgagcg gctcgccgga ttgcgacttg
     2041 atctcgaatt tgtacagcgc gtcttctgtg agcccaggga tgaagagatc ccatatgccg
     2101 ttcccgggat ggtttcgcat cgggtggcgc cggccgtccc agtgattgaa atctccgacg
     2161 acgctgactc ttttggcatt gggggcccag accgcaaagc acaccccagg cacgccaccg
     2221 agggttatca ggtgggcccc caacttttca tagtttctga gatggctgcc ctcgccaatc
     2281 aggtgcaggt catagtcact caaggtcgga ggaaaacggt aagggtcctc gatctcgcat
     2341 ccctgtccct gccgatctac tacacgcagt cgatagggaa agaggccgtc ccgcgtcggg
     2401 actacgacct cgaagaggcc atccgggtgc accagctcca tgggctggtc cttgctcacg
     2461 ccatcagcat ggacgaccgt cgcctcccgg gcatccggga ggaaggcgcg cacggcgacg
     2521 catggctcag aggcgacgcg cacctcatgc gggcccagca cagcgaacgg atcgccatgc
     2581 tcaccctgga cgatcgcctc gat
//
LOCUS       DATMXI010000267         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_111186, whole genome shotgun sequence.
ACCESSION   DATMXI010000267 DATMXI010000000
VERSION     DATMXI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111186"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /gene="mutL"
                     /locus_tag="VN444_05055"
     CDS             <1..1481
                     /gene="mutL"
                     /locus_tag="VN444_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWR21213.1"
                     /translation="ATRQDTTVGSQFTLVAGGSGSVVDAPAVPGTRIEVSNLFFNQPA
                     RRKFLKSATTEFSHISRVVQLAGLAWPSVHFRLTHNGQEILNYPAVTTDRDRILQVYR
                     PAFLDRTIEARGRIAGMSIRGVMVDPLHARSSRTPQELFVNRRPIRNATVFHAIVDGY
                     GSFLPKGHHPTFVLFLDIEPDRLDVNVHPTKKEVRFADTEALHQLVRQTVRHALDGSE
                     RKVVLGLTQGGASQTIPESLPTAVRQVDLLSGSRQAGLIPATGRDSRIMMKDTETLSG
                     IVEGSQVAFAHEPVAMYQRAEKPDIVPLGQILRTYLVAQVGDELQVIDQHTAHERVLF
                     QRLWRGWQSREIPSQPLLIPEPVELPAAQSLLLLKRSDDLEKLGLLVEPFGATGVAIR
                     GVPVGLGKVDAAVLVQDLLEDLTEWDRASSLELRVQPVLASMACHGAVRAGRTLALPE
                     IQQLIYDWVQEGLIMTCPHGRRTAFRLSTDELAQLFGRVGWS"
     gene            1478..>2286
                     /gene="miaA"
                     /locus_tag="VN444_05060"
     CDS             1478..>2286
                     /gene="miaA"
                     /locus_tag="VN444_05060"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWR21214.1"
                     /translation="MMTIPAKATCRLSGKQVCCKPVVVIVGPTAVGKSRIAVEVAKTF
                     ETEVLTADSRQVYRGMDIGTDKPFPEERQGVPHRLIDLVGPGELFNAGVYRRQAVDEI
                     ERLYKDRRLPLVVGGTGLYVRTLLKGLCSAPPSDPILRKVLQQEAIDQGHDRLYARLA
                     AVDPMVAAQLHPRDESKVIRALEVYQLSGRRMSEFQQEHGFTERPFSALIIGLNRDRG
                     ALYRRIESRIDWQLAHGLIEETRQLLAQGYRRESGAMKGLGYRQVAEHLAG"
BASE COUNT          461 a          616 c          705 g          504 t
ORIGIN      
        1 ctgcaacaag gcaagacacc actgtgggat cgcagttcac cctggtcgca ggcggcagcg
       61 gttccgtcgt ggacgccccc gccgtgccgg gcaccaggat cgaagtgtcc aatttgtttt
      121 tcaatcagcc agccagaagg aagtttctga aatcagccac gacggagttc tctcatatca
      181 gccgtgtcgt tcaactcgcc ggccttgcct ggccatcggt tcatttccgt ctgacccata
      241 acggccagga gattctcaac tatcctgccg tcaccacgga tcgtgatcgg atccttcaag
      301 tctatcgccc agcctttctc gatcgaacta ttgaggcgcg gggtcggata gcaggaatgt
      361 cgatccgtgg cgtgatggtc gatccgcttc atgcgagatc ctcgagaaca ccacaggaat
      421 tgtttgtgaa tcggcggcct atccgcaatg cgactgtgtt tcatgcgatc gtggatgggt
      481 atgggtcgtt cctgcccaag gggcatcacc caacgttcgt gctcttcctg gatatcgaac
      541 ccgaccgact cgatgtcaac gtacacccta cgaagaaaga ggtccgtttt gcagacacgg
      601 aagctcttca tcagttggtg cgtcagaccg tccgtcatgc actcgatgga tctgagcgga
      661 aggttgtgct gggtctgacg cagggagggg catcgcagac gataccggag tcgcttccta
      721 ctgccgtgag gcaggtcgat ctactcagtg gctctcgcca ggctggcttg atcccagcca
      781 ctggccggga ttcccgaatc atgatgaagg atacggagac cctatccggc atcgtcgaag
      841 gaagtcaggt tgccttcgct cacgagccag ttgcgatgta tcagcgggcg gagaaacctg
      901 acatcgtgcc actcgggcaa atcctccgga cctatctcgt ggctcaggtg ggtgatgaac
      961 tgcaagtgat cgatcaacat acggcgcatg agcgtgttct tttccaacgg ctttggagag
     1021 gctggcagag ccgggaaatc ccgtcgcagc cgctcctgat tccggaaccg gtcgaactgc
     1081 cggcagcgca gagtctactg ctgctcaagc gcagcgacga tctggaaaaa ctcggtctgc
     1141 tggttgaacc ttttggcgca acaggcgtgg cgattcgggg agtgccggtc gggttgggca
     1201 aggtggatgc ggcggtgttg gtacaggacc tgctcgagga cttgaccgag tgggatcgag
     1261 cctcgtcctt ggaattacgc gttcaaccgg tcttggcgtc tatggcctgt catggggccg
     1321 tacgcgctgg gcgtaccttg gcgcttcctg agattcagca gttgatctac gattgggtac
     1381 aggaagggtt gatcatgacc tgcccgcatg ggcgtcgcac ggcctttcgt ctttccacag
     1441 atgaactcgc ccagctgttc ggcagggttg gatggtcatg atgaccattc cagcgaaagc
     1501 aacctgccgt ctctcaggga agcaagtctg ttgcaagcct gtggttgtga tcgtgggtcc
     1561 gactgctgtg gggaagagcc gcattgcggt cgaggtagcc aagactttcg agaccgaggt
     1621 gctcacggca gattcccgtc aagtctatcg tgggatggac attgggacgg ataagccatt
     1681 tccggaggaa cgccaggggg ttccccaccg actaatcgac ctcgtcggtc ctggtgagct
     1741 atttaatgcc ggtgtatatc gacgtcaggc agtggatgag atcgaacgcc tgtacaaaga
     1801 ccgccgtctt cctctcgttg taggcggcac agggctctat gtaaggaccc tcctgaaggg
     1861 cttgtgtagc gcgccaccgt ccgatccaat tctgaggaag gttctgcagc aggaggcgat
     1921 tgaccaagga cacgatcgcc tctatgctcg acttgcagcg gtcgacccaa tggtcgcggc
     1981 gcaattgcat ccccgcgacg aatcgaaagt aatccgtgcg ctggaagtgt accagttatc
     2041 cggtcgccgg atgtcggagt ttcagcagga acatggattt accgaaaggc cattttcagc
     2101 cttgatcatc ggcttgaacc gggaccgcgg tgcgctgtat cggcgtatcg agagccggat
     2161 tgactggcaa ctggctcatg gtttgataga agaaaccaga cagctcctgg cccaggggta
     2221 tcggcgtgag agtggggcca tgaagggttt ggggtatcgc caggtggcag agcacttggc
     2281 cggtag
//
LOCUS       DATMXI010000268         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_161829, whole genome shotgun sequence.
ACCESSION   DATMXI010000268 DATMXI010000000
VERSION     DATMXI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161829"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /gene="trmFO"
                     /locus_tag="VN444_05065"
     CDS             complement(<1..1256)
                     /gene="trmFO"
                     /locus_tag="VN444_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HWR21215.1"
                     /translation="MTDVVVVGGGLAGSEAAWQAAERGASVRLHEMRPVSSTPAHKTD
                     RMAELVCSNSLKSDSPEDCHGLLKRELTSYGSVIMLAARAHAIPAGSALAVDREAFAA
                     EVTARLAQHPRITIVREEVKAIPEEKPVIIATGPLTSDRLAGALRDRFVDYLASADPS
                     ANTRDLLYFYDAISPIIAGDSIDRTVAFAASRYDKGGDDYLNCPMTREEYRLFWQSIR
                     AAELTPIHPFEEARYFEGCLPIEVLAARGEDTLRFGPMKPVGLIDPSTGRQPYAVVQL
                     RLENREGTMSNMVGFQTRLKWDEQRRILRMIPGLSDAEFLRYGSIHRNTFIAAPALLQ
                     ETMQYKGDPGIFLAGQLTGVEGYLESAGSGLLAGVNAVRFLRGEAPVVLPPTTALGSL
                     IHHICHADPRTFQPMNVNFGLLPPLDA"
     gene            complement(1253..>2911)
                     /locus_tag="VN444_05070"
     CDS             complement(1253..>2911)
                     /locus_tag="VN444_05070"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWR21216.1"
                     /translation="RAMQIAQQLYEGIEIGEEGAVGLITYMRTDSVRVSGEAQAEAAR
                     YIAERFGQAFVPERPPVYRSGRGAQEAHEAVRPTSVYRDPASLRGFLTKDQLALYSLI
                     WNRFVASQMPPALYDVTTVAIQGGRFTLRASGRHQRFAGFMQVYIEGKDEASKTKPEE
                     TKDETAEEAAVEEEAELAVPPLEVGEPLRLIGVTPAQHFTQPPPRFTEATLVKELEEQ
                     GIGRPSTYAAILSVIQNRDYVVKAQGTFRPTELGGIVVDLLVESFPRVMDYEFTAKME
                     TTLDEIEEGQKQWLEELHRFYDAFSQWVKEAAVGMRNIKAMEEKTDEVCERCSSQMVI
                     RWGRFGRFLACTNYPECKGTRELPPELKEETGIEEEANAAGEAKAEPCDNCGRPMVMK
                     RGRFGAFLGCSGYPECKTIRKLSQAEASSARPAPQPTNEVCDKCGNPMVLREGRYGRF
                     LSCGSYPTCKNIKPISIGVNCPQCRSPLSERRTKRGRVFYGCTAYPKCAFALWDRPVQ
                     EPCPRCGAAFLVEKKLKGGGVSIRCAAEGCAYVRETDAAVKANT"
BASE COUNT          536 a          955 c          865 g          555 t
ORIGIN      
        1 gcatcaagcg gcggcagcag gccgaagttc acattcatcg gctggaaagt gcgggggtcg
       61 gcgtggcaaa tgtgatggat gagggacccg agtgcagtgg tcggaggaag tacaactggg
      121 gcctcacctc ggaggaatcg aaccgcatta actccggcca ggagtccgct tccggccgat
      181 tccaggtacc cctccacgcc ggtgagctgt ccggccagga agatcccagg atcgccttta
      241 tactgcatcg tctcctggag tagcgccggc gcggcgatga aggtgttgcg atggatcgat
      301 ccgtatcgga ggaactcggc gtcgctgaga ccgggaatca ttcggaggat gcggcgctgc
      361 tcgtcccact tcaggcgggt ctgaaacccc accatgttag acatcgtccc ttcccggttc
      421 tccagccgga gctgcacgac ggcgtagggc tggcgtccgg tcgagggatc gatcaggccc
      481 accggtttca tcggtccgaa ccgcaacgtg tcctcgccac gagccgccag cacctcgatg
      541 gggagacacc cttcgaagta tcgcgcctct tcgaacgggt ggatcggtgt cagctccgcg
      601 gcccggatcg actgccagaa gaggcgatac tcctcccggg tcatcgggca attcaggtag
      661 tcgtcccctc ctttgtcgta gcgggatgcg gcaaacgcca cggtacgatc gatagaatcc
      721 ccagcgatga tcggcgagat ggcgtcatag aagtagagca gatcccgcgt attggcagac
      781 gggtctgctg atgccagata gtccacgaac cgatcacgca gcgcgccggc cagccggtcg
      841 gaggtcagtg gaccggtggc gataatcacc ggcttctctt ccggtatcgc cttgacctcc
      901 tcccgaacga tggtgatgcg gggatgttgc gcgagccgcg cggtcacctc ggcagcaaag
      961 gcctcacgat caacggccaa ggccgatcct gccgggatgg catgcgccct ggcagcaagc
     1021 atgatcaccg atccatacga ggtgagctcc cgtttcagca ggccatgaca atcctccggc
     1081 gaatccgact tcagggagtt gctgcacacc aactcggcca tccggtcggt cttgtgggcg
     1141 ggggtggaac tcaccggccg catctcatgc aggcgcacgc tggcgccccg ctccgccgcc
     1201 tgccaggcgg cttcgctccc ggctagacct ccaccaacta cgacaacatc ggtcacgtat
     1261 tcgcctttac tgccgcatcg gtctcccgta cgtaggcaca tccttcggcg gcacaacgga
     1321 tcgagacccc gccgcccttc agcttcttct ctaccaggaa ggcggctccg caccgtggac
     1381 agggttcctg gaccggacga tcccagaggg caaaggcgca cttgggatac gcggtacacc
     1441 catagaagac ccggccccgt ttggtgcgtc gctccgagag agggctcctg cattgcggac
     1501 agttgacccc aatcgagatg ggctttatgt tcttgcaggt agggtagctc ccgcacgaaa
     1561 ggaagcggcc gtaccgtccc tcccgcaaca ccatcgggtt gccgcacttg tcacagacct
     1621 cattagtcgg ctgcggcgca ggccgcgcgc tcgaagcctc cgcctgcgag agcttccgga
     1681 ttgtcttaca ctcagggtat ccggagcaac ccaggaaggc tccaaaccga ccgcgcttca
     1741 tcaccatagg ccgcccgcag ttgtcgcagg gttctgcctt ggcctcgcca gcggcgttag
     1801 cctcctcctc gatcccggtc tcctctttga gctctggcgg caattcgcgg gtccccttgc
     1861 actcagggta attggtgcag gccaggaagc ggccgaaccg gccccagcga atcaccatct
     1921 gactgctgca ccgctcgcag acctcgtcgg tcttttcctc catcgccttg atatttcgca
     1981 tcccgaccgc ggcttccttc acccactggg aaaaggcgtc atagaaacga tgcaactcct
     2041 ccagccattg cttctgcccc tcttcgatct catccagggt ggtctccatc ttggccgtga
     2101 actcgtagtc catcacgcga gggaagctct ccaccaggag atcgacgacg atcccgccca
     2161 gctcagtagg ccgaaatgta ccctgcgcct tcactacata gtcgcggttc tggatgacac
     2221 tcaggatagc cgcataggta gagggtcgtc cgatcccctg ctcttccaac tccttcacca
     2281 gggtcgcctc tgtgaagcgg ggcggcggct gagtaaagtg ctgggcaggg gtcacaccaa
     2341 tgagccgcag cggttctcca acctccagag ggggtaccgc taattcggcc tcttcctcga
     2401 cggccgcctc ctcagctgtt tcgtctttgg tctcctcggg cttcgtcttg gacgcctcat
     2461 ccttcccctc gatgtagacc tgcatgaatc cggcgaatcg ctggtgccga ccgcttgccc
     2521 gaagcgtgaa ccgcccaccc tggatcgcga cagtagtcac gtcgtagaga gccggcggca
     2581 tctgagacgc aacgaagcgg ttccagatca ggctgtagag ggcgagttga tccttcgtca
     2641 gaaacccccg gagcgatgcc ggatcccgat agactgaggt cgggcgaact gcttcgtggg
     2701 cttcctgggc gccacggccg ctgcggtaga ccgggggtcg ctcaggcaca aatgcctgac
     2761 caaaccgttc ggcgatatag cgggccgcct cggcctgggc ttctccagag acgcggaccg
     2821 agtcagtccg catgtaggtg ataagaccaa cggcgccctc ctcgccgatc tcgatccctt
     2881 cgtagagttg ctgagcgatc tgcatcgccc g
//
LOCUS       DATMXI010000269         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_297722, whole genome shotgun sequence.
ACCESSION   DATMXI010000269 DATMXI010000000
VERSION     DATMXI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297722"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VN444_05075"
     CDS             <1..182
                     /locus_tag="VN444_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HWR21217.1"
                     /translation="LGASWGFMLPVSTPPNAIVYGSGMVPITAMIRAGIFFDILGGLV
                     IWAGLYFLLPMAGLA"
     gene            complement(405..1223)
                     /locus_tag="VN444_05080"
     CDS             complement(405..1223)
                     /locus_tag="VN444_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWR21218.1"
                     /translation="MKTKWGRIETRAWATGSYFPLGTVLLIAMVALMLAATVQELRAE
                     SEIPFSRTRMIIEYNSSADDIGVQVSLDGEPWRSLKIENPNGRTILDIIANRSLRKQG
                     LTELFFESSEPSLADVPIEEFFARFPEGVYEFEGTTVEGQAIEGEAVFTHVIPAGPVI
                     VSPLEGAEVNPKALDIVWNRVTETRDGDPVKIVAYQVIVERTDNAELGAAPRLFDIKL
                     PPSPGEPQRVTVPSQFLEPGKEYKFEVLAIEEGGNQTITEGGPFTTKSAPIRAR"
     gene            complement(1556..>2092)
                     /locus_tag="VN444_05085"
     CDS             complement(1556..>2092)
                     /locus_tag="VN444_05085"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HWR21219.1"
                     /translation="DGTLSNGLLVEAIGILDAVGAEMSATEIGPGDEISVADANEIEV
                     TGFVTALFSLSEFTVGNQRVQSDVGTLFVDGTQADVALGAKIEVSGILSNGVLYATEI
                     EFWQPDQIEFEGPVAAIASANEFTVGSQLVRTDSGTVFEGGTPEDIVVGVLLEIKGVP
                     VDTARSIVVADKVSFENE"
BASE COUNT          415 a          617 c          551 g          509 t
ORIGIN      
        1 ccctcggcgc cagctggggc ttcatgctgc cggtctccac gccgccgaat gcgatcgtct
       61 acggctcggg catggtgccg atcacggcga tgatccgggc cggcatcttt tttgacatcc
      121 tgggcgggct ggtgatctgg gccgggctct acttcctgct gccgatggcc gggctggcat
      181 agtggtgccg gtgaaaagct gcattctttt tttccttaat caattttaat gggaggccct
      241 cgatacgagg cgagcctggt tcagcctgag atgtgataac ctactaaagt ccaatccaaa
      301 taaaagaggc agagcaaaag ctctgcctct tttttctcga accgactctt gtttcttatt
      361 cggatcagga ttctggattc atttcacgga gacccttaac ggacctatct ggctcgaatc
      421 ggagcactct tggtcgtgaa gggacctcct tccgtgatgg tctggtttcc gccctcctct
      481 atcgccaaca cttcgaactt gtactctttg cctggctcca ggaattgcga gggaaccgtt
      541 actctctggg gctctccggg gctgggcgga agtttaatgt caaatagccg cggggcggcg
      601 ccgagctcgg cattatccgt ccgctcgacg atgacctggt aggcgacaat cttaacaggg
      661 tcaccatcgc gcgtctcagt aacacgattc cacacgatat caagcgcctt agggtttacc
      721 tctgcgccct ctaaaggaga gacaatcacg ggtccggccg gtatgacatg ggtgaatacg
      781 gcctcccctt ctatcgcctg tccttcgacc gtcgtcccct cgaattcgta aactccctcg
      841 ggaaagcggg caaagaattc ttcgatgggg acatcggcaa gcgacggctc cgagctctcg
      901 aagaacagct cggtcaggcc ctgttttctc aggctgcggt tggcgatgat gtccaggatc
      961 gtccgcccgt tggggttttc gattttgagc gaccgccagg gttctccgtc gagagagacc
     1021 tgaacaccaa tatcgtccgc cgaggagttg tactcgatga tcatcctcgt cctgctgaac
     1081 ggtatttcgc tctctgcccg gagctcctgg acggtggccg ccagcatcag ggccaccatc
     1141 gcgatcagaa gaacggtccc caaagggaaa taggacccgg tggcccatgc ccttgtttca
     1201 attctgcccc attttgtttt catcgcaccc acctctttct ttggtttgga gtttccggtt
     1261 agtggtttcg ttttccaatt gaagaaatga cagagttgga tttgacaatt tcgccgatgc
     1321 ttcgcggttg cacctccttt gttgggatta aggtgtcgca ggggtccttt ggggccatag
     1381 gggctccccg tcagagtcat aaaaaaaggc cccccgcgtt gatccggaga gcctgttgac
     1441 agtatgcgcc tgcttgcgta tatttgtacg caatcgagcg ccgcaaggcc ctcgggacct
     1501 ccaagccaag caccctgacc cgccaaagtc gcgtgcttgg cttttttatt tttccctatt
     1561 cgttctcgaa actgactttg tcggcaacga caatgctgcg agcggtgtct accggaacgc
     1621 ccttaatctc aagcaagaca ccgacaacaa tgtcttcggg cgtcccccct tcgaagactg
     1681 tgccggaatc cgtccgcacc aactggctgc cgacagtgaa ctcgttagcc gaagcgatag
     1741 cggcaaccgg accctcgaac tcgatctgat ccggctgcca gaactcgatc tccgtggcgt
     1801 ataggacccc gtttgacaga atgcctgaaa cctctatctt ggcaccgagg gctacgtccg
     1861 cctgcgttcc atccacaaac aaggttccca catcggactg tacccgctgg ttccccacgg
     1921 tgaattcgga tagcgaaaac agggcagtga caaaaccagt cacttcgatc tcattggcat
     1981 cggcaacgct gatctcgtct ccgggtccta tttctgttgc agacatttcc gcgccgacgg
     2041 catcaaggat ccctatcgct tcgaccagca acccattgga tagtgttccg tc
//
LOCUS       DATMXI010000270         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_107329, whole genome shotgun sequence.
ACCESSION   DATMXI010000270 DATMXI010000000
VERSION     DATMXI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107329"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VN444_05090"
     CDS             complement(<1..361)
                     /locus_tag="VN444_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21220.1"
                     /translation="MSTIPLLWYFVYAPVHELSHLLGAYLVGGQIVEVKLIPRFWAGE
                     TGGAWIKSEGVANEWSQLIMTIAPYFLDVVSSAVGVYVLQRKLSRNAFLIGFLFMVLC
                     LRPFFDVVCETIGFATGF"
     gene            complement(651..971)
                     /locus_tag="VN444_05095"
     CDS             complement(651..971)
                     /locus_tag="VN444_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21221.1"
                     /translation="MKYHHLGIPTLVPHPGETYLEKHKTFCTDNENNPFGIQWMRYEE
                     DCPLPEIVKRIPHVAFEVEDLQEALAGQTILIKPNSPSDGVLVAFIECNGAPIEFLQF
                     VKQP"
     gene            complement(1189..1692)
                     /locus_tag="VN444_05100"
     CDS             complement(1189..1692)
                     /locus_tag="VN444_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWR21222.1"
                     /translation="MNTLPDSSKSGGALPPDFTQRIEREVGRVFNTQAPSLVFRNVFS
                     GSQDSLTSLRGKVVMVSIWNSGCKPCIAEMPALFAVQSELGGRGFVLLTLSGEDTTRQ
                     KRFFAARKLTHGGIAGSMRTQDYEYPFQYLINPAGYIIDRKGVLMDFWLGAKTQDELV
                     KKIMKYL"
BASE COUNT          480 a          577 c          524 g          496 t
ORIGIN      
        1 tgaagcctgt cgcaaatcca atagtttcac atactacgtc aaaaaaaggt ctcaaacaga
       61 gcaccatgaa gagaaatccg atgagaaacg cgttacgtga gagcttccgt tggagaacgt
      121 acaccccgac tgcactgctc acaacgtcga gaaagtaagg cgcgatcgtc atgatcaact
      181 ggctccattc gttggcgacg ccctctgatt tgatccacgc cccgccagtc tcccctgccc
      241 aaaacctagg aatgagtttg acttcgacaa tctgaccacc aacaaggtat gcgccaagca
      301 gatggctcaa ttcatgcaca ggcgcgtaga cgaaatacca cagcaatgga atggtagaca
      361 tgcacgccac catcgtttgt tgtttccagg ttcccctgag ataaagcaca agaaggggaa
      421 caacgacggt gaagagtatt tcgaacgtga tcaacagatg gttcatgaca atggcggcaa
      481 tttaacgggt ggcggctcac ttacggctga tggacctctg agtcccccac cgcatctcat
      541 gagccgtatc acttcatgat tgacgtgcat ctttagcctt gctcaaaccc ggtcacttcc
      601 ccacgaccga attgtccgtc aggtgcagcc gcttgcgagg ccgctcgaac tcagggttgc
      661 ttgacgaact gaagaaactc gatcggtgcg ccgttgcact caatgaatgc tacaagaact
      721 ccatctgaag ggctgttggg ttttatgaga atggtctgtc cagcgagtgc ctcctgtaga
      781 tcctccactt caaacgccac atgtggaatg cgtttgacta tttcaggaag gggacagtct
      841 tcctcgtatc gcatccactg gatgccaaac ggattgtttt cattatccgt gcagaatgtc
      901 ttgtgtttct caaggtaggt ctctccgggg tgcggcacaa gcgtcgggat gccaaggtgg
      961 tggtacttca tcggtgtagt atctccgtgc attcttaccg gttgccgtga gggccgccta
     1021 acgggcggag gctcacctgc ggcagatgga catcacaccc cacgacggca cttactgacc
     1081 cgtcccgtgc ttttgatcga cgtgaaactt gaacattgct caaacgctgt cgctctccac
     1141 caccggatct tccgtcaggc gcagcgcttg ttaggtcacc gcgtcggact acagatactt
     1201 catgatcttc ttgacaagct cgtcctgagt cttggcaccg agccagaagt ccatcaagac
     1261 tcctttgcga tcaataatgt accccgcggg atttatcagg tactgaaatg ggtattcata
     1321 gtcttgggtt cgcattgagc cggcaatacc cccgtgagtg agttttctcg ccgcgaagaa
     1381 ccgtttctgt cttgtggtgt cctcgccgga aagcgtgaga aggacgaatc ctctcccacc
     1441 aagttcggac tgtactgcaa agagggctgg catctcagct atgcatggct tgcatccgct
     1501 attccatatg ctgaccatga caaccttccc acgcaatgat gtgagtgaat cctgactccc
     1561 tgagaatacg ttgcgaaaca cgagagaggg agcttgagta ttgaaaactc gtccaacttc
     1621 tctctcgatc ctctgtgtga agtctggagg aagcgcaccg cctgattttg aactatcagg
     1681 aagggtattc atcacccagt cctgggcaaa cgctgaacag gtgcagagga cgacaaccaa
     1741 ggacatacga attgcgttca tctttgtgtc cctccatgag atttcaatcg cagcggccta
     1801 actggctgcg gcccacctgc gattgatgga cctttcggtc ctctaccgac cgtcctgagc
     1861 cgtaccgctt tttgactgat gtgaatcttg aactttgctc acactttgtc acttcccctc
     1921 cgccgactct tccgtcaggc gcagccgctt gttaggcggt gggactttcc ggccactccg
     1981 cgtcgggtcg gcgaggttgc ggctgatcct cacccaatcg ggctgctgaa atctcagcat
     2041 agatgaaatt ctccggtggt acgggactgc cgcacaa
//
LOCUS       DATMXI010000271         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_177152, whole genome shotgun sequence.
ACCESSION   DATMXI010000271 DATMXI010000000
VERSION     DATMXI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177152"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1838
                     /locus_tag="VN444_05105"
     CDS             201..1838
                     /locus_tag="VN444_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21223.1"
                     /translation="MVATFLVIGIMCWLVMFSPVEAASADQIEASPLFDDLGSLHHPI
                     TTTSELAQQYFDQGLRLVYAFNHEEAIRSFEAAARQDPQAAMPYWGIALALGPNINSE
                     MGRQDERRAIEMVQKARRLATNAIPREQAYIEALTARYVGRKGVKRKGLDEAYAKAMR
                     LVMQQFPDDLDAATLFAEALMDLRPWDLWKQDGRPQPGTEEIVTTLESVLAQNPDHPG
                     ACHYYLHAVEASQQPERALPCAERLPGLMPGAGHLVHMPAHIYMKLGKYHEAVERNQE
                     AAHVDQLYLAGRNQGGEYADGYYTHNLHFLWASLMMEGRNDDALKAARDLTATIALEE
                     VRKDRGKELYLSAPIFSMIRFGRWEELLREPAPPKGLRLLDGMWRLGRGLALVATGRL
                     PGAEGEHVVLAGLTKQIRRDRTPEEKTERALLKIAERVLAGELAARRQHYDDAIRLLE
                     EAAKMEEALPYSEPPLWPLSVRHHLGAVLLSADRPSEAEAVYHADLLHHPDNGWALTG
                     LIQSLSIQQKDDQAAEAEERFKKAWAHADYSPSNSRM"
     gene            2040..2660
                     /gene="msrA"
                     /locus_tag="VN444_05110"
     CDS             2040..2660
                     /gene="msrA"
                     /locus_tag="VN444_05110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWR21224.1"
                     /translation="MRRRATLSVFALAIFLIGSWTVQSASQAAGNGIPSKAYFAGGCF
                     WCMEEAFEKVEGVLSATSGYMGGTVANPSYEAVSAGRTGHAESVEVVYDPAKVSYQRL
                     LDAFWHNVDPVTPNAQFCDHGSQYRSAIFFQTEEEKRASDASKQAIEQSKRFNEPIVT
                     QIVMASQFYPAEEYHQDYYKKNPFRYKVYKYNCGRAKRLEELWGNP"
     gene            complement(2805..3185)
                     /locus_tag="VN444_05115"
     CDS             complement(2805..3185)
                     /locus_tag="VN444_05115"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21225.1"
                     /translation="MSQGRVLIVDDESDVRTSVRLILTKAGYDVDEAEDPEAGVALVK
                     STRSQVPLSAIVTDLNMPKINEIIVIPYYRSQYPTCPIIVLSGSPKLEKASNMFKAAG
                     VEFLPKPINQEQLLSALKNAVKAG"
     gene            complement(3344..>3605)
                     /locus_tag="VN444_05120"
     CDS             complement(3344..>3605)
                     /locus_tag="VN444_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21226.1"
                     /translation="SYPSGFSAQAIICDINLPKVSGHEFIAFIRQQLPTVPVIVLTGY
                     PDVQGAAALFKQGVVDYLIKPGRADTLLDAVRRAMGEQALIG"
BASE COUNT          788 a          971 c         1044 g          802 t
ORIGIN      
        1 agcaagcttc tcgtgccggc gatgacgctc aacgacttca acttttccag cgtcactcga
       61 ttctgaggtt tgcccggagt ggagtttctc gtttcatgtt tcacgtttgg ccttgatgtt
      121 tccgcaattc gaaacgaaca acttgaaatc agaaacatga aacttgaaac gctcgaatga
      181 gatacgttgt acgaggatcg atggtcgcta ctttcttggt gatcggtatt atgtgttggc
      241 tcgtgatgtt ctctccggtg gaagctgcgt ctgctgacca gattgaagct tcgccgctct
      301 tcgacgatct tggttccctc catcatccga tcaccactac atcggaattg gcccaacagt
      361 actttgatca ggggctccga ctcgtctatg ccttcaatca cgaagaagcg atccgttcgt
      421 ttgaagctgc ggctcgtcag gatccgcagg cggctatgcc ctattggggg attgcattgg
      481 cgctgggccc caatatcaat agtgagatgg ggaggcaaga tgaacgtcgt gcgatcgaaa
      541 tggttcagaa ggcccgacgg ctggcaacca acgcgattcc acgggagcaa gcgtatatag
      601 aggcgttgac tgcgcggtac gtgggccgga agggtgtgaa gcgtaagggt ctcgatgagg
      661 cctatgcgaa ggccatgcga ttggtgatgc agcagtttcc cgatgacctc gatgccgcca
      721 cgctgtttgc ggaagcgctg atggatctcc ggccctggga tttgtggaag caggacggga
      781 gaccacagcc gggcaccgag gagatcgtca cgacgttgga gtccgtactc gcgcagaacc
      841 ccgaccatcc aggagcctgc cactactacc ttcatgcagt cgaggcgtca cagcagccgg
      901 aacgggcttt gccctgtgcg gaacggttgc ccggtctgat gccgggagcc ggtcatcttg
      961 ttcatatgcc cgcccacatt tacatgaagc ttgggaagta ccacgaagcg gtcgaacgga
     1021 atcaggaggc ggcccatgtc gatcaactat atctggccgg ccggaaccag gggggcgaat
     1081 atgcagacgg gtattacacc cacaatctcc attttctctg ggcctccttg atgatggagg
     1141 ggcgcaatga cgatgctctg aaagcggcgc gcgacttgac cgccacgatc gcactggagg
     1201 aggtccggaa ggatcgcggg aaagaactct acctatcggc gccgatcttc tcgatgatac
     1261 ggtttggccg gtgggaggag ttactccggg aaccagcgcc tcccaaggga ttgcggttgt
     1321 tggatggaat gtggcggctg ggtcgagggc tcgccctcgt ggcgacaggt cggcttcccg
     1381 gagctgaagg ggaacatgtg gtcttggccg gtctcacgaa gcagatccgt cgtgatcgta
     1441 cgccagaaga aaagactgag cgggcgcttc tcaagattgc tgagcgggtt ctggcaggag
     1501 agcttgctgc gcgccgtcaa cattatgatg acgcgatccg gttgcttgaa gaggcagcca
     1561 aaatggaaga agcgctcccc tattccgagc cgccgctctg gcccctgtcc gtgcggcatc
     1621 acttgggcgc tgtgctcttg tcggccgatc gcccatccga agctgaagcg gtgtatcacg
     1681 cggatctcct acaccatccg gacaacggct gggccttgac cggactaatc cagagcctca
     1741 gtatccaaca gaaagacgat caggctgctg aggcagagga gcgtttcaag aaagcctggg
     1801 cccatgcaga ctacagtccg tctaattctc ggatgtaagg cggagcgagc cgagcgagat
     1861 gtgcgcgaca ggcgcgacgt gcaaagaagt tctgagttat caggaaccaa ctcagcactc
     1921 agaattcata actcagaact gggtttattt ggcccgtccc gcttttctcg caagtctcgc
     1981 atgtgaggat gtaagacctc acacacctat tcgactgaat cagtgaggag gacaatccca
     2041 tgagaagacg agcaaccctg tcagtttttg ctctggccat tttcctcata ggatcttgga
     2101 ctgttcagtc ggccagccag gctgcgggga atggaatccc cagcaaagcc tacttcgcgg
     2161 ggggctgttt ctggtgcatg gaagaagcct ttgaaaaggt cgaaggggtg ttgtccgcga
     2221 cttcaggcta tatggggggt acggttgcta atccgagcta tgaagcagtg tcggctgggc
     2281 ggacgggcca tgcggaatcg gtcgaagtcg tctatgaccc ggccaaggtg agttaccaga
     2341 ggttactgga cgccttttgg cataatgtcg atccggttac accgaatgca cagttctgcg
     2401 accacggcag ccagtatcgc tccgctattt ttttccagac agaggaagag aaacgtgcgt
     2461 cggacgcctc caaacaggcg atcgaacagt cgaaacggtt taatgagccg atcgtcacgc
     2521 agatcgtgat ggcctcacag ttctatccgg ccgaggagta ccaccaggac tactacaaga
     2581 aaaatccttt ccgttacaag gtctacaaat acaactgcgg tcgcgccaag cggttggagg
     2641 aattgtgggg aaatccataa cccgcatgat tcaagcgcgg ttctgctact gcgatgttgg
     2701 agactggcac ccttcttttt ggtgcctgtc tccgtcctag gggtgggaca ggcaccctcc
     2761 tcagcccaga ggcgacggag gggccagtcc ctcttgtctt tcaattaccc ggccttgacg
     2821 gcattcttca gggcgctcaa caactgctct tggttaatcg gttttggaag gaattccaca
     2881 ccggctgcct tgaacatgtt ggaggctttc tccagcttcg gtgatccact cagcacaatg
     2941 atcggacagg tggggtattg ggaacgataa tagggaatca cgatgatctc gttgatcttc
     3001 ggcatattga gatcggtcac aatcgcactc aagggcacct gactacgtgt tgatttcacc
     3061 aaggcgactc ctgcctctgg atcttcggct tcatccacat cgtatcctgc cttcgttaag
     3121 ataaggcgca cggatgttcg cacgtccgac tcatcgtcca cgataagcac tctcccttga
     3181 ctcataatgc ctcccctctg tattaggccc ttagtcccct aaccccttcc tcagtccgcg
     3241 gctatcaggt atcacgggat cacgatatct gcctatcccc tcaaatgagg ggggtggctc
     3301 ccaggatgcg cgagagatac gagacacgcc gccgcgtggc ggtctacccg atcagcgcct
     3361 gttcacccat agccctcctg actgcatcca acaacgtatc tgccctgcca ggcttgatga
     3421 gataatcgac tacaccttgt ttgaacagtg ccgcagctcc ctggacatcg ggatacccgg
     3481 tcagcacgat gacgggaacg gtcggaagct gctgccggat gaatgcgatg aactcgtgcc
     3541 cgctcacttt gggaaggtta atgtcacaga taattgcttg ggctgagaag ccggagggat
     3601 aggat
//
LOCUS       DATMXI010000272         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_200911, whole genome shotgun sequence.
ACCESSION   DATMXI010000272 DATMXI010000000
VERSION     DATMXI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200911"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VN444_05125"
     CDS             <1..236
                     /locus_tag="VN444_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21227.1"
                     /translation="AGIRLEGQDRADTRSLASFDGELIEAGKAKQVQTVETAFNRHGM
                     LEIIGEGGAGQQQRAVQSKYYYAPEAVFDLPLE"
     gene            309..551
                     /locus_tag="VN444_05130"
     CDS             309..551
                     /locus_tag="VN444_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWR21228.1"
                     /translation="MAKSKIAISLDENTLDRLDRLVQGEVFPNRSQAIQVAVEERLER
                     LKRGRLAQECAKLDPAFEKALAEEGLSGDISEWPEY"
     gene            536..877
                     /locus_tag="VN444_05135"
     CDS             536..877
                     /locus_tag="VN444_05135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWR21229.1"
                     /translation="MARILRGEIRWANLNLVRGKEQTRLRPVLILSHDVFNERSGTVI
                     AVAITSQPQRAGFPLTLELSAKGFPKQSWAKISQVRTLAVERIGKRLGDATSEEVSQV
                     IEGFIEIMGGV"
     gene            877..1458
                     /locus_tag="VN444_05140"
     CDS             877..1458
                     /locus_tag="VN444_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWR21230.1"
                     /translation="MSTIRDEVLAANKAYAVQFGEKGKLPMPPGRRFAILTCMDARLD
                     PAKYAGLSEGDAHVIRNAGGRASDDAIRSLVISYKLLGTREWFVIHHTNCGMETFTDE
                     IMRGLLVRSLKTATIDAGGWHDTGQGPGSTEGAFIDWLTVGDQVESVCADVQRIRAHP
                     LVPHDIPVYGYIYDVRTGQLVEVPEATRIGKAA"
     gene            1775..2206
                     /locus_tag="VN444_05145"
     CDS             1775..2206
                     /locus_tag="VN444_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWR21231.1"
                     /translation="MKIVSWYASDKIIYESEKETIRDTVIEAVRQEFPLMNADLRNAN
                     LRDADLQGAILGGADLRGANLQSTNFRGANLRDANLSGANLQSTNFRDANLAGTNLGD
                     TNLGDADLFHVKLWGRGGLTKIKKAQVESFLKALGIVVEDE"
BASE COUNT          555 a          588 c          687 g          515 t
ORIGIN      
        1 atgcgggaat ccggctggaa ggtcaagatc gggcggacac acgctcgctc gcttcatttg
       61 acggtgagtt gattgaagcg ggaaaagcga aacaggttca gacggtggaa acggccttca
      121 accgacacgg aatgctcgag atcatcggtg aaggcggcgc cggccagcag cagcgcgccg
      181 ttcagagcaa gtattactac gcgccggaag ccgtcttcga cctgccacta gagtaggctc
      241 agtccttatg cctgtgcttg acttggccag tggtatgaca tattgtaata ctcatggagg
      301 tgccgcagat ggctaagtca aagatcgcaa tatctctcga tgagaacacc cttgatcgac
      361 tcgaccgtct tgtgcaaggt gaggtcttcc cgaatcgcag ccaggcgatc caggtagcgg
      421 ttgaagagag gttggaacgc ctgaagcggg gccgtcttgc tcaggaatgc gcgaaactgg
      481 atcctgcctt tgagaaggcc cttgcggagg aggggctttc gggggatata tccgaatggc
      541 ccgaatactg aggggggaga tccgctgggc gaatctgaat ctggtccgtg gaaaagagca
      601 gacgagattg cgcccggttc tgatcttaag ccacgacgtg ttcaatgagc gctccgggac
      661 agtgatcgct gtggcgatca ccagccagcc ccagcgtgcg ggttttccgc tgaccctgga
      721 attgagtgcg aaaggttttc cgaaacagtc gtgggccaag atcagccagg tccgcacgct
      781 ggccgtggaa cgaattggga agcggttggg agacgccact tcggaagagg tgtctcaggt
      841 tattgaaggg ttcatagaaa tcatgggagg agtctaatga gcacgattcg agatgaggta
      901 ctcgccgcca ataaggccta tgcggtacag tttggtgaga agggtaagct acccatgccg
      961 ccgggacgac gatttgccat cctgacctgt atggatgcgc ggcttgatcc ggccaagtac
     1021 gcgggactat cggaggggga tgcccatgta attcgcaatg cgggtggtcg cgccagcgac
     1081 gacgccatcc gatccctggt catttcctac aaacttctcg ggacgcggga atggttcgtc
     1141 atccaccaca ccaattgcgg tatggagacc tttaccgacg agatcatgcg cgggctgctg
     1201 gtgaggagcc tcaaaaccgc gaccatcgat gccggcggat ggcacgatac tggtcaaggc
     1261 ccagggtcaa cggaaggggc atttatcgac tggttgaccg tcggggacca ggtcgagagt
     1321 gtctgtgcgg atgtacaacg cattcgggcg cacccgctag tccctcacga catccccgtc
     1381 tatggctaca tctacgacgt gaggaccggg cagcttgtcg aggtccctga agccacacga
     1441 atcgggaagg cagcgtaaaa gatcctgata catcttgcta tattattttc gtaacgactc
     1501 agtatagata ggctgaaggc ttttaggggt aaggcgtgca tgatcataca aaactccaaa
     1561 aaggtcttga atggctcggc tcgtgcctta cgagataatt tagggccttc agccttcagt
     1621 ctaagcagct aagtagttac ttattttctt gacacgtcgc tgagcccaat tgtattaact
     1681 gcgtctagtc gtgttttgtt ctacggtagg ggaggatgcg gccatggcgg ttggcaggaa
     1741 cccgaaaccg aagcagtaac aataggtaac tattatgaaa attgtttctt ggtacgccag
     1801 tgataagatt atttatgaat cagagaaaga gacgattcgc gataccgtga tcgaagcagt
     1861 cagacaagag ttccccctca tgaacgccga cctgagaaat gccaatctca gggatgccga
     1921 tcttcagggc gccatcctcg gtggcgccga tctgagaggt gccaacctcc agagcaccaa
     1981 cttccggggc gctaacctcc gggacgccaa cctctctggt gccaacctcc agagcaccaa
     2041 cttccgggac gccaatctcg cgggcaccaa cctcggggac accaatctcg gagacgccga
     2101 tctctttcac gtgaaattgt ggggacgtgg cggtttaaca aaaattaaga aagcacaagt
     2161 cgagtctttc ctgaaagcgt taggaatagt tgttgaagac gagtaactac ttaggcgttt
     2221 aggctgaagg cttttaggtt tagctctctg cttccttcag cctatctacc tgtcttcctc
     2281 agaaaatgca ttttcgtgcg ccgtggtacg gcgatgtcgc atgagccgtt gcttacacct
     2341 tacta
//
LOCUS       DATMXI010000273         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_22113, whole genome shotgun sequence.
ACCESSION   DATMXI010000273 DATMXI010000000
VERSION     DATMXI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22113"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            580..717
                     /gene="rpmH"
                     /locus_tag="VN444_05150"
     CDS             580..717
                     /gene="rpmH"
                     /locus_tag="VN444_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005307156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWR21232.1"
                     /translation="MSFTFQQPSNLKKKREHGFRKRMSTKNGRKVLARRRAKGRARLA
                     V"
     gene            888..1250
                     /gene="rnpA"
                     /locus_tag="VN444_05155"
     CDS             888..1250
                     /gene="rnpA"
                     /locus_tag="VN444_05155"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWR21233.1"
                     /translation="MPQGGGDRDLFLKRSLDIEYAKEHGRRISTSLFNMVICRADQGS
                     SRIGIVVGKRFGGAVSRNRAKRLFRELTRQLRGHLLTGHSLVVFPKRDSIVQPFAVLK
                     EVWLSSLRRQRLLRSDED"
     gene            1319..3019
                     /gene="yidC"
                     /locus_tag="VN444_05160"
     CDS             1319..3019
                     /gene="yidC"
                     /locus_tag="VN444_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWR21234.1"
                     /translation="MDKRIIVFIVLSLGVILGFDLFMRQMGWMPEPPPAENTTATDSS
                     SQGQEPASTNVTSQSGTPTGSSIPTQSGQKSGAPSPANSPTILEQTITVETDLVRIGL
                     SNRGGVIQSLELKRYHTKPPEAKPVQLVYHGGKFRGPLSITVPNADIDKTIREGLYSI
                     EKDFTSLDAAHPVGHVTMQFRDPVTHLSVEKRLTFHHGSYMVDVAVAMEGVTESYEVG
                     LGTNFGIVEWGDGFIGLIGSASRVDDKIDKDTPEKELELKGAVQWVALQDKYFLTVMM
                     PKQGEAVLVKTEAPKVVSAGIRMPASGSTSSVALQLYAGPKEYDTLRSLNVGLEDMID
                     FGWFIYGSWTIVKSVAKPIFYVLRFIHEYIPNYGVCIILLTLGIKLLFVPLQYKSYKS
                     MKMMKAIQPKIAALQEKFKGDRDRLNKELMKLYREQKVNPLGSILPMLLQMPVFVALF
                     NVLYMSIDLRQAPFMLWVTDLSVEDPFYVLPIIMGATMFIQQKITPNTMDPTQAKIML
                     ILPIGMTFLFINFPAGLVLYWMTNNILTITQQLVTDRFLLHDKALIAAGTEQSRSTNA
                     "
     gene            3028..>4405
                     /gene="mnmE"
                     /locus_tag="VN444_05165"
     CDS             3028..>4405
                     /gene="mnmE"
                     /locus_tag="VN444_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWR21235.1"
                     /translation="MRGGLDDTICAIATPAGQGGIGIVRLSGPHSLSIASQVVRLRSG
                     DSLSSVPSHTLHLADLVIPALEKHGEVRAVRNRLPVSGLIDEALVVYMKAPRSFTAED
                     VVEIQSHGGMLVLELVCRVCLESGARMAEPGEFTKRAFLNGRLDLSQAEAVLDTIRAA
                     SSAGLNIAQRQLRGDLAHEIEQARASLLTVLAHVEAGIDFVDEDISFLQQDEFVRIVS
                     HARVVVQKLEATARDGRILKEGAHVVILGRPNVGKSSLLNRLLKEERAIVTAIPGTTR
                     DVIEESIDIDGVMIHLADTAGMRETDDVVEREGIRRARAAQDEADLLLVVVDGSVPLT
                     SDDRELLRSVRDRKHMVLLNKADLAETVERDPVLTDRLVYTMSAKTGMGVELVKSALL
                     AQLVSGGFEAGESLMVTNARHRDALRRASTSLDQALESVQGGMAGELISIDVRAAADA
                     LGEITGAIT"
BASE COUNT          955 a         1104 c         1302 g         1044 t
ORIGIN      
        1 gtgtaatcgg cactgtgaaa tgattccagc ctgacaggat ccacgcgatc ttcgtgccag
       61 tcgaacaggg ccaagtaggt tttcgtaccc ccgatatctc cggcaagaat catcctaccc
      121 tcacgcgctt gtactccgat gctctggagg ctcggaagca actgctcagt cgagatgcca
      181 aatgaggcgg ccgccctctg gtgctaccaa tgatgcagga agctgccgtt ctgcctcggt
      241 ctttggattg aggatggctc atacaactcc tgctttgccg gctcccacga cgagaaacag
      301 tatcacactc gcctggattg atcacaccta aagtcattgt caatctggtc ggaggatctt
      361 ttggagattg ggtcacggag atcaggcttc ggttgtcccg atggtattgt ggatcgacgg
      421 atgggaaata tcccgcttca tgcgactgtc atggccgaga tgaccggtga tcgcgagact
      481 tgttttcttg ccgacgggct agcacctcgt ttgacaggct ggcggcccct ccctataata
      541 gcctgttatt cgtgagtctt ttaggccagg agtgattgta tgtcgtttac atttcagcag
      601 ccatcgaacc tcaagaagaa acgtgaacat ggatttcgta agaggatgag cacaaagaac
      661 ggccgaaagg tcttggctcg tcgccgtgcg aaggggcggg cccgcttggc cgtataggtc
      721 aggcacgctg gctagggctt cacgaaggaa ttttccccct ccgttacgtc ccccagactc
      781 ggtagttccc aacagcatga tgacattgtg tggtcattcc cgagtccgga gatcgggcct
      841 cgaaatggat gtgcggtacg aacctgcctt ctcccaggat gatggtcatg ccgcagggtg
      901 gaggcgatag ggatctgttt ctcaagcgca gcctcgatat cgagtatgcc aaagagcatg
      961 gtcggcgtat ttcgacctcc ttgtttaata tggtgatttg ccgggctgac caggggagca
     1021 gccggattgg aattgtggta ggaaagcgat ttgggggcgc agtcagccga aatcgagcga
     1081 agcgcttgtt tcgggaactc actcggcaat tgcgcggcca cttactcaca ggacacagcc
     1141 ttgtggtttt ccccaaacgt gattcgatcg tgcaaccctt tgctgtcttg aaagaggtgt
     1201 ggctatcatc gctccggcgc caacggttgc tccgtagcga tgaggactag cctgcgctga
     1261 tccagtgaat tcgtagagtc agcattgctc atgttcacat cagggtagcg gtttgcttat
     1321 ggacaaacgc attatcgtct ttatcgttct ctcgttaggg gtcatcctgg ggtttgactt
     1381 gttcatgagg cagatggggt ggatgcctga accgcctcct gcggagaata ccacagccac
     1441 ggactcttct tcccaggggc aagagccagc ttccaccaac gtgacgagtc agagcggtac
     1501 cccgaccggt tcgagcattc caactcaatc cggccaaaaa tccggcgccc cttcaccggc
     1561 taattctccg acaatattgg aacagacgat cacggttgaa accgatctgg tccgcatcgg
     1621 actttcgaat cgtggtgggg tgattcaaag cttagaactg aagcgctacc ataccaagcc
     1681 tcccgaagcg aagccggtac agctggtcta tcatggcggg aagtttaggg gcccgctctc
     1741 gattacggtc ccgaatgctg acatcgataa gacaattcga gaagggctct atagcattga
     1801 gaaagacttc acaagtctcg atgccgctca tcccgtcggg catgtcacga tgcaattccg
     1861 tgatccagtc acccatctca gcgtggagaa acggctcacg ttccaccatg gcagttacat
     1921 ggtggatgtc gcggtcgcca tggagggagt gactgagtcc tacgaggtcg ggttggggac
     1981 gaacttcggt atcgtggaat ggggtgatgg atttatcggt ttgatcggct cggcctctcg
     2041 tgtagacgac aaaatagaca aggacacacc tgagaaggag cttgagctga agggggcggt
     2101 tcagtgggtc gccttacagg ataaatattt cctcacggtc atgatgccaa agcagggtga
     2161 ggccgttctc gtcaagacgg aagctccaaa ggtcgtgtcg gcagggatac gaatgcccgc
     2221 atcaggatca acatcctcgg ttgcacttca gctgtatgca ggcccgaagg agtatgacac
     2281 gctccgatcg ttgaacgtgg gcctggagga catgatcgat ttcggatggt tcatctacgg
     2341 gagttggacc attgtgaaat ccgtggcgaa gccgatcttt tatgtgctga gatttattca
     2401 tgagtatatc cctaactatg gggtctgcat catcctgctc acgctgggga tcaagctgct
     2461 ctttgtgccg ttgcaataca agagttacaa gtcgatgaaa atgatgaagg cgatccaacc
     2521 caagatcgct gcgctccagg aaaaatttaa aggtgatcgt gaccgcctga acaaggagct
     2581 gatgaagctg taccgggagc agaaagtcaa tccgctgggc agtattctcc cgatgctgtt
     2641 gcagatgccg gtcttcgtgg ccctgttcaa tgtcctctac atgtcgattg atttgcgtca
     2701 agctcccttc atgctctggg tgaccgattt gtcggtggaa gatcccttct atgtgctgcc
     2761 gataatcatg ggcgcgacca tgtttattca acagaagatt acgcccaaca cgatggatcc
     2821 gactcaggcg aagatcatgt taattcttcc gatcggtatg acctttctgt tcataaactt
     2881 tcccgctggc cttgtcttgt attggatgac gaacaatatt ctgactatca ctcaacagtt
     2941 ggtgacggat cggtttctgc tgcatgacaa ggcactcatc gcggcaggga ccgagcagag
     3001 ccgcagcacg aacgcataga agtcatcatg cgtggagggc tcgacgacac gatctgtgct
     3061 attgccaccc ctgcaggcca aggcggcatc gggatcgttc gtttaagcgg ccctcactcg
     3121 ctttcgatcg cgtcccaagt tgtccggctt cggtctggtg attccctctc atccgttcct
     3181 tcccatacac tgcatcttgc cgatcttgtc atacctgcat tggagaagca tggcgaagtt
     3241 cgtgccgttc gtaaccgcct gcccgtatcg ggcctgatcg atgaagcgct cgtggtctat
     3301 atgaaggccc cgcgatcgtt cacggctgaa gacgtggtgg agattcagtc gcatggggga
     3361 atgttggtgc ttgagctggt gtgccgggtc tgtctggagt ccggtgcaag gatggctgag
     3421 ccgggggagt ttacgaagcg ggcttttctc aatgggcgcc tggatctctc ccaggcagaa
     3481 gcggtgctcg atacgatcag ggccgcatca tccgccggat tgaatatcgc gcagcgccag
     3541 ctacggggtg atctcgctca tgagatcgag caggctcggg cctccctcct gactgtgctg
     3601 gcccatgtcg aggcagggat tgattttgtt gacgaagata tttcctttct tcaacaggat
     3661 gaattcgtac ggatcgtgag tcacgcccgt gtagtcgtcc aaaaactgga agcgacggcg
     3721 cgggatggac gaattttgaa ggaaggagca cacgtcgtca tcttgggacg cccgaatgtt
     3781 ggtaagtcca gtttgttgaa ccggcttctg aaggaagaac gtgcgattgt aactgcgatt
     3841 cctgggacaa cgagggatgt gatcgaagaa tccatcgata ttgacggggt gatgattcat
     3901 ctggcggaca cggccggcat gagagagacg gacgatgtgg ttgagcggga aggcatcaga
     3961 cgtgcgcgtg ccgcacagga tgaggcagac ctgttgttgg tggtcgtcga tgggtcggtg
     4021 cccctcacga gcgatgaccg ggagctgttg aggtcggtca gggatcgcaa gcacatggtg
     4081 ttgctgaata aggcggatct ggctgaaact gtggagaggg accccgtgct gactgaccgc
     4141 ctggtctata ccatgtctgc aaaaacaggg atgggggttg agttggtgaa gtcggctctt
     4201 ctcgctcagc tcgtgtccgg aggttttgag gcaggagaaa gtctcatggt gacaaacgca
     4261 agacatcgtg acgcgttacg ccgggctagt acttccttgg accaggctct cgagtctgtg
     4321 caaggcggga tggccgggga acttatttcg atcgatgtgc gggctgcagc cgacgcgctt
     4381 ggggaaatca cgggcgccat tacga
//
LOCUS       DATMXI010000274         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_134442, whole genome shotgun sequence.
ACCESSION   DATMXI010000274 DATMXI010000000
VERSION     DATMXI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134442"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..>2551
                     /gene="gyrA"
                     /locus_tag="VN444_05170"
     CDS             190..>2551
                     /gene="gyrA"
                     /locus_tag="VN444_05170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWR21236.1"
                     /translation="MPDEQQGALGRIGEVRPTDIHEEMRRSYMDYAMSVIIGRALPDV
                     RDGLKPVHRRVLYAMQELGLPFNRPYKKAARVVGEVLGKYHPHGDTAVYDTLVRLVQD
                     FSMRYPLIDGQGNFGSVDGDSPAAMRYTEVRLARIAQEMLRDIDKETVDWAPNFDDTL
                     QEPTLLPAALPNLLVNGSSGIAVGMATNIPPHNLGETVDALLLQLDDPEVTLDRLMEA
                     LPGPDFPTAAYIHGRQGIRDAYTTGRGLIRMRAKAFVEKGRGGRESIIVSALPYQVNK
                     AKLIERIAELVRDRKIEGISDLRDESDREGMRIFIELKKDQMAPPILNQLYKHTPMQS
                     TFGVIMLALVNNQPKVLALKEMLHHFIEHRKTIVIRRTRFDLRKAEERAHILEGYRIA
                     LDHLDAVIALIRRSRSVDDARTGLMDQFGMSQIQAQAILDLRLQRLTQLERQKIQDEY
                     SEILATIERLRSILVSDALVRQIVKEELLAVKDTYGDPRRTQILEETADIDLEDMLAD
                     EEMVITITHGGYIKRSNLNVYRSQRRGGKGTTGMATKEEDYVEHLFVATTHSYILLFT
                     NRGRVHWLKVHELPQLGRAAKGKALVNFLQLGAGETMTTVIPIRQFEVDRYLLMATKR
                     GIIKKTELNAYDNPRTGGIIAITLDEGDELIAVRMTKGDDEVLLGTRRGMAIRFKEEE
                     ARPVGRAARGVIGISLEEGDAVVGAEVVSPGAAVLTVTEHGYGKRTEPEEYRLQGRGG
                     KGIINIRTTERNGPAVGVMQVQPGDQIMMISQEGKITRMRVDEISLI"
BASE COUNT          547 a          732 c          835 g          437 t
ORIGIN      
        1 acagaggtaa gcagcctgga tatctaactc agagttcatg gttcagggtt catggacgaa
       61 gggcgaccct tggttgtcgg cagaatcggg ctggtttacc atgaaccatg aactatcgac
      121 tatgaacccc catcgcgtgg aggcccggcg atcgggccgt tttcatttgt gtggctcgcg
      181 gaggagtgaa tgcctgacga acaacaaggt gcgctaggcc gaattggtga ggtgaggccg
      241 accgacatcc acgaggagat gcgccggtcg tacatggact acgccatgag cgtgatcatt
      301 ggccgggcct tgcctgacgt acgggacggg ctgaagccgg tgcaccgccg ggtcctgtac
      361 gctatgcagg agttgggact cccctttaac cgcccataca agaaggcggc ccgcgtcgta
      421 ggtgaggtct tgggcaagta ccacccacat ggcgatacgg cggtctatga caccctcgtt
      481 cgtctggtcc aggatttctc gatgcggtac cccctcatcg acggccaggg aaacttcggc
      541 tcagtggatg gcgactcgcc ggcggcgatg aggtatacag aggttcgcct tgccaggatc
      601 gcccaggaga tgctccggga catcgacaag gagacggtgg actgggctcc caactttgac
      661 gacacgcttc aagagccgac gctcctgccg gcggcccttc caaacctgct cgtcaacggc
      721 tcttccggga tcgcggttgg gatggccacc aacatcccgc cccacaatct cggcgaaacc
      781 gtggacgccc tcctgttgca gctcgatgat cccgaggtga cgctggatcg gctcatggag
      841 gcgctacctg gaccagactt tccaacggcc gcgtacatcc acggcaggca ggggatccgg
      901 gatgcctaca cgacaggacg aggcctgatc cgtatgcgtg cgaaggcgtt tgtggagaag
      961 ggcaggggcg gcagggagag catcatcgtc tcggcgctcc cgtaccaggt gaacaaagcc
     1021 aagctgatcg agcggatcgc tgagctggtt cgggacagga agatcgaggg gatttcggat
     1081 ctgcgggacg agtcggatcg cgaggggatg cggatcttca tcgagctcaa aaaggatcag
     1141 atggccccgc cgatcctgaa ccagctctac aagcacaccc cgatgcaatc aaccttcggt
     1201 gtcatcatgc tggcccttgt gaacaaccag ccgaaggtgc tggcgctgaa ggagatgctc
     1261 catcacttca tcgagcaccg gaagaccatc gtcattcgca ggacgcgctt cgacctccgg
     1321 aaagccgaag agcgggccca cattttggag gggtaccgga tcgcgttaga ccatctggac
     1381 gcggttattg ccctcatccg ccggtcccgg tcggtggacg acgcccgcac cgggttgatg
     1441 gaccagttcg ggatgagcca gatccaggcc caggcaatcc tcgatcttcg tctgcagcgc
     1501 ctgacacagc ttgagcggca gaagatccag gacgagtaca gcgagatcct tgccacgatt
     1561 gagcgccttc gtagcatcct ggtcagtgat gccctggttc gccagatcgt caaagaggag
     1621 ctgctggccg tgaaggacac gtatggggac ccgcgccgca cgcagatcct ggaggagacc
     1681 gccgacatcg atctggagga catgctggcc gacgaggaga tggtgatcac cattacgcat
     1741 ggcggctata tcaaacggag caatctcaat gtataccgga gccagcgtcg aggcggtaag
     1801 gggacgaccg gcatggcgac caaggaagag gactacgtgg agcacctctt cgtcgccacc
     1861 acccacagct acatcctcct cttcaccaac cgggggaggg ttcactggct caaggtccac
     1921 gagctgcccc agctcgggcg ggccgcgaag ggcaaggctc tcgtcaactt cctgcaactc
     1981 ggagccggcg aaacgatgac gaccgtgatt ccgatccgcc agttcgaggt tgatcgatac
     2041 ctcctgatgg cgaccaagcg cgggattatc aagaagactg agctcaacgc gtatgacaac
     2101 ccgcgcacgg ggggcatcat cgcgattacg ctcgatgaag gggacgagtt gatcgcggtc
     2161 cgcatgacca agggagacga cgaggtcctg ctcgggacta gacgggggat ggcgatccga
     2221 ttcaaagagg aagaggcccg gccggtgggc cgggcggccc gaggcgtcat cgggatctcc
     2281 ctggaggagg gcgatgcggt ggttggggcc gaggtggtgt ccccaggcgc ggcggtcctc
     2341 acggtgaccg agcacggcta tggcaagcgg accgagcccg aagagtaccg gctgcagggc
     2401 cggggcggca aagggatcat caacattcgg accaccgaaa gaaacggtcc ggcggtcgga
     2461 gtgatgcagg tacagcccgg cgatcagatc atgatgatct ctcaggaagg aaagattacg
     2521 cggatgcggg tggacgagat cagcctcatc g
//
LOCUS       DATMXI010000275         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_229634, whole genome shotgun sequence.
ACCESSION   DATMXI010000275 DATMXI010000000
VERSION     DATMXI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229634"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VN444_05175"
     CDS             <1..132
                     /locus_tag="VN444_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR21237.1"
                     /translation="LPLGRFGRPEEVAAVVIFLASERASMVTGACVTVDGCQSRSLI"
     gene            complement(151..2643)
                     /locus_tag="VN444_05180"
     CDS             complement(151..2643)
                     /locus_tag="VN444_05180"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWR21238.1"
                     /translation="MSNGRPEQYTGSVSRKHGHAALTVSALQDAIRYHVRYSLGKRWE
                     SCSGRELFTAVAFTVRDSLIDRMLDTEDRYQLADPKRVHYLSMEYLPGRSLKNNLSNL
                     GMLDLCRDALHKMGVNLDQVLEGECEAGLGNGGLGRLAACFLDSLATMSMPAYGYGIN
                     YEYGLFKQDIDNGYQKEKPDNWLTHGSPWQIEHPDEACIVPLYGRIEHGIDRTGQYNP
                     MWMDWKMIIGVPHDMPIVGFGGQTVNVLRLYSAQSSEEFDIQIFNDGDYLKAVEQQIS
                     SETISKVLYPSDLVEAGRELRLIQEYLLVACTLRDIVRRYEKSHATFDQFASKVAIHL
                     NDTHPALAVAELMRILVDEHAQPWEKAWETTQAALSYTNHTLMPEALEKWSVSLFEYV
                     LPRHLQIIYEINRRFLTQVASMWPGEHERLRHMSLIEEGEHKAVRMVNLAIVGSHATN
                     GVSDLHTHMVKTSLVPDFYQLWPDKFSTKTNGITPRRWLLQANPLLADLITSTIGNGW
                     ITDLGKLRALEPYAQDPDFQQAFIKVKRSSKAILTRVIHDRCRVAVNPDALFDVHVKR
                     IHEYKRQLLNVMQIIHQYLRMTQDGTIPPVPRVYIFAGKAAPGYWAAKQLIKLITSVG
                     QVINNDPRVQGLIKVVFIPDYRVSLAEQIVPAADVSEQISMAGTEASGTGNMKFAMNG
                     ALTIGTHDGATIEIMEEVGQENTFLFGLQVEEIRKMRERGSYHPRSYYQRNPNIRRLM
                     DTFASDLFCPNQPGLFRWIYESVLDRGDPYFHLADLESYIETQELVAQAFKDPAVWAC
                     KAILNVARMGKFSSDRAIAEYAREIWNIKPIL"
     gene            complement(2647..3117)
                     /locus_tag="VN444_05185"
     CDS             complement(2647..3117)
                     /locus_tag="VN444_05185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21239.1"
                     /translation="MSGKHITLAITSDLQNVPLIGQAVEQLCALIPLSDIESHQIALC
                     VVEAVTNAIVHAYGKETEHKIEVIFGQYPDHLNLQICDTGRAMDPKRLDPQAPSPLDF
                     DPCRLNAVPEKGLGLAIIKATMDEVTYTTCEGKNILTLTRLFRPCQAEAEPLRG"
     gene            complement(3131..3472)
                     /locus_tag="VN444_05190"
     CDS             complement(3131..3472)
                     /locus_tag="VN444_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWR21240.1"
                     /translation="MQLEQRKAGEALVVAPLEERLDARVATDFKERMAELIASGNTRI
                     VLDLSRVEFIDSSGLGAIVSSLKRMGGRGDLVVCGLQETTMTLFRLTRMDRVFQVFDS
                     EQQAVSALGGS"
     gene            complement(3490..3804)
                     /locus_tag="VN444_05195"
     CDS             complement(3490..3804)
                     /locus_tag="VN444_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWR21241.1"
                     /translation="MGEPEMVVYVDEELADLIPEFLENRRRDVEAITRLVQEGKHAEL
                     TRLGHTMKGTGGGYGFPEISEIGQGIEEAGARGDRKAITTLCERLATYLATVAVKVRR
                     QE"
BASE COUNT          821 a         1169 c         1034 g          914 t
ORIGIN      
        1 ctgccgcttg gccgattcgg tcgccccgag gaggtcgcag cggttgtgat cttcctagcg
       61 tccgaacgag ccagcatggt gaccggagcg tgtgtcacgg tcgatggctg tcagtcgcgg
      121 tcgctcatct aaccggccgc gtcgggcggg ctagaggatg ggcttgatgt tccagatctc
      181 ccgggcgtac tcagcgatgg cgcggtcgct ggagaattta cccatcctgg caacgttcaa
      241 gatcgccttg catgcccaga ccgccgggtc tttgaacgcc tgagcgacca gttcctgggt
      301 ctcgatatac gactccagat cggctagatg gaagtacggg tccccccggt ccaggacaga
      361 ctcataaatc cagcgaaaca ggccaggctg atttgggcaa aacaggtctg atgcaaacgt
      421 gtccatgagc cgtcttatat tggggttgcg ctgatagtag gatcgaggat gatatgatcc
      481 tcgctcacgc atcttccgga tctcttcgac ctgtaatccg aagaggaagg tattttcctg
      541 acccacctct tccatgatct cgatagtggc cccatcatgg gtaccaatgg tgagcgcccc
      601 attcatcgcg aacttcatgt tccccgtgcc tgaagcttcc gtcccagcca tggaaatctg
      661 ctcgctcaca tctgcggcgg gaacgatctg ttccgccagg gagacacggt agtctggaat
      721 gaagaccacc ttgatcagcc cttgaactcg gggatcgtta ttgatgacct gacctacact
      781 ggtgatcaat ttgataagct gcttcgccgc ccaatacccc ggcgccgcct tacccgcaaa
      841 gatatacaca cgcggcacag gaggtattgt cccatcctgg gtcatgcgca gatactggtg
      901 gatgatctgc atgacgttga gcaattgccg tttgtactcg tggatccgtt tgacatgaac
      961 atcaaacagc gcatcgggat tgactgccac ccggcagcgg tcatggatga cccgggtgag
     1021 gatcgccttg ctggatcgct tcacctttat gaatgcctgt tggaaatccg gatcctgtgc
     1081 atagggttcc agggctctga gcttaccaag atcagtgatc caaccgttcc caatggtgct
     1141 ggtgatgaga tcggctaaca gaggattggc ttgaagcaac cagcgtcgcg gcgtgatccc
     1201 attcgtcttc gtgctaaatt tgtccggcca gagctgatag aaatctggca ccagggaggt
     1261 tttcaccatg tgggtgtgga gatccgacac cccatttgta gcatggctgc cgacaatcgc
     1321 caggttgacc atgcgcacag ctttatgctc cccctcctca atcagtgaca tgtgtcgtaa
     1381 tcgctcgtgc tcacctggcc acatggaggc cacctgcgta aggaaccggc ggttgatctc
     1441 gtaaatgatc tgaagatgcc gtggcaagac gtactcgaac agtgagaccg accatttctc
     1501 cagcgcttcg ggcatcaagg tgtgattggt atacgacagt gcggcctgag tcgtctccca
     1561 ggctttctcc cagggttggg catgttcgtc aaccagaatg cgcatcaatt ctgcaacggc
     1621 cagggctgga tgggtatcgt tgaggtgaat ggcgaccttg gaggcgaact ggtcaaaagt
     1681 agcatgactc ttttcatacc gcctgacaat gtctctaagg gtacatgcca caaggaggta
     1741 ttcttgaatc agtcgcagtt cccggcccgc ttcgaccagg tcagaggggt agaggacctt
     1801 ggagatggtc tccgaggaga tctgctgttc gaccgccttc aggtagtctc catcattgaa
     1861 gatctggata tcaaattcct cggacgattg cgccgagtac aatcgcaaca cattcaccgt
     1921 ctggccaccg aaccccacga tcggcatgtc gtgtggtaca ccgatgatca tcttccaatc
     1981 catccacata gggttgtatt gaccggtgcg atcgatgccg tgttcgatgc gaccgtacag
     2041 aggaacgatg caggcctcat cgggatgctc aatctgccag gggcttccgt gggtcagcca
     2101 gttatccggt ttttctttct gatacccatt gtcgatgtcc tgtttgaaca agccatactc
     2161 atagttaata ccgtatccat acgccggcat actcatggtg gccagggagt ccagaaaaca
     2221 ggctgctaag cgaccgagcc cgccattgcc cagaccggcc tcacactcgc cctccagtac
     2281 ctgatcgaga ttgacaccca tcttgtgcag cgcatcccgg caaagatcca gcatccccag
     2341 attgctcagg ttatttttca aggaccgacc ggggagatac tccatggaaa ggtaatgcac
     2401 gcgcttggga tcggccagtt gataccgatc ctcggtgtcc aacattctgt cgatgaggga
     2461 gtctcgaaca gtaaatgcga cagccgtaaa cagctcgcgg ccggaacagc tctcccacct
     2521 tttgcctaat gagtaccgga catgatagcg gatcgcatct tggagggcgg ataccgtcag
     2581 ggccgcatga ccgtgcttgc gggataccga ccccgtatac tgctcaggtc tcccgttcga
     2641 catcggtcac cctctcaatg gttctgcttc ggcttgacac ggtctgaaca acctggtcag
     2701 ggtcaggata tttttcccct cgcaggtggt atacgttact tcgtccatcg tcgctttgat
     2761 aatggccagt cccaacccct tttcgggaac agcattcagg cgacaaggat caaaatccag
     2821 gggtgaaggc gcctgaggat cgaggcgctt cggatccatg gctcttcccg tatcacagat
     2881 ctgaaggttc agatggtcgg gatactgtcc gaagatcacc tctatcttat gctctgtctc
     2941 cttgccgtag gcatgcacga tggcattggt gacggcctcg accacgcata gcgcgatctg
     3001 gtgtgattcg atatcggaaa gcgggataag ggcgcagagt tgctctaccg cctgaccgat
     3061 gagcggcaca ttctgtagat cgctggtgat ggccagtgtg atgtgttttc cgctcacgtc
     3121 gcctcatcct ctatgagccg cctaaagctg agacggcctg ctgctcactg tcaaagacct
     3181 gaaacacccg gtccatcctt gtcagccgga acaaggtcat agtggtctcc tgcaacccgc
     3241 acacgaccaa atccccccgt ccccccattc gcttcaggct tgagacaatc gctcccaggc
     3301 cgctactgtc aatgaactcc accctggaca gatccaacac aatcctcgta ttcccggaag
     3361 cgatcagttc agccatccgc tccttaaaat cagtcgcgac tctggcatcc agccgctcct
     3421 ctagcggcgc aaccaccaac gcctcgcctg cttttcgctg ctcgagctgc atcgcatgtg
     3481 ctcctttgcc tattcctgcc gccggacctt caccgcaaca gttgccagat atgttgctag
     3541 tcgctcgcac agcgtcgtga tggcctttcg atcgccccgc gcgccggcct cctcgattcc
     3601 ctgtcctatc tcgctgatct ctgggaaccc atagccgcct ccggtccctt tcatcgtatg
     3661 gcctaaccgc gtgagctccg catgcttacc ttcctggacc agccgcgtga tcgcctcaac
     3721 atcccggcgc cgattctcta aaaactccgg gatcaggtct gccagttcct cgtcaacata
     3781 gacgaccatc tccggctccc ccatctccat ctccttccga aaatcccact tatcaaatcc
     3841 ccacgtggcc cttctttgct aaggggggag tggggggtcg taatcggtca gatagcgggc
     3901 caccgtagca agcatgagct ccttcctcag cggctttg
//
LOCUS       DATMXI010000276         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_262584, whole genome shotgun sequence.
ACCESSION   DATMXI010000276 DATMXI010000000
VERSION     DATMXI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262584"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /gene="tyrS"
                     /locus_tag="VN444_05200"
     CDS             <1..941
                     /gene="tyrS"
                     /locus_tag="VN444_05200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWR21242.1"
                     /translation="EIQANAETYKRQIFQILDQSRTEIRFNSEWLSRMDFAHVIRLAA
                     QYTVARLLERDDFQKRYREGRPIGVHEFLYPLAQGHDSVVLRADVELGGTDQKFNLLV
                     GRELQRDHGQEPQVALITPLLEGTDGVQKMSKSLGNYIGIGEPAREVYGKTMSIPDAL
                     IVKYAELVAGMSPEAIEAMEEGLKLGTLHPRIAKAEVARKLVALYHGERAAEEATCEF
                     DRIFREKGLPDDINLFMVADEMTAVDIAWLIKESGGTRSLSEAKRLIRQGGVSLDGEV
                     VRDEYAKILMDREYLLRVGKRFFRRVKKNAQPLNNT"
     gene            1180..>2291
                     /locus_tag="VN444_05205"
     rRNA            1180..>2291
                     /locus_tag="VN444_05205"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          556 a          529 c          749 g          457 t
ORIGIN      
        1 aggagattca ggccaacgca gaaacctata agaggcagat ctttcagatc ctcgatcaaa
       61 gccgaaccga gatccgtttc aatagcgaat ggctgagtcg aatggacttt gcccacgtga
      121 tccgcctggc ggcgcagtac acggttgctc ggcttctgga gcgggacgat tttcaaaagc
      181 gctatcggga ggggcggcca attggcgtcc atgagttcct ctacccgctg gctcaagggc
      241 atgactccgt ggtactgagg gccgatgtcg agttgggtgg taccgatcaa aagtttaacc
      301 tgcttgtggg gcgggagcta cagcgggatc atggccagga gcctcaggtt gcgctcatca
      361 cgcccctgct tgaaggaacc gatggggttc aaaagatgag caagagcctc ggcaactaca
      421 ttgggattgg tgagccagcc agggaggtat acgggaagac gatgtcgatc ccggacgcgc
      481 tcattgtgaa atatgccgag ctggtagccg ggatgtcgcc ggaggctata gaggcaatgg
      541 aagaaggact gaagcttggc acgcttcatc ccagaatcgc taaagcagag gtggccagga
      601 agctggttgc gctctatcat ggagagcggg ccgccgaaga ggcgacttgc gagttcgatc
      661 gtattttcag ggaaaagggg ctaccggacg acatcaatct tttcatggta gcagatgaga
      721 tgaccgccgt tgatatcgcg tggttgatta aggaatcagg agggacgagg agcctctcag
      781 aggccaagcg gctcatccgt cagggtggcg tcagtcttga tggggaagta gtgcgggatg
      841 aatatgcaaa gatcttaatg gatcgggagt acttgctgag agtgggaaag cgatttttta
      901 gaagggttaa aaagaacgct caaccactaa ataacacttg acagccataa agcagttgct
      961 gtatgctccc attcgtagtt gtcagtgcct gccattgaca cctgggactt gatgccatat
     1021 actaatccat agcttgatgg gcattaaggc gcaacagctt aatacctgct ctttgacaat
     1081 agaatattgt tgctactgac ttgatggcga atgaattcag ccagagcggg tgacccggta
     1141 acggagagcc tgcttgtagg ctgttactat gtagtttcca atttggagag tttgatcctg
     1201 gctcagaacg aacgctggcg gcgtgcctaa cacatgcaag tcgagcgaga aagtcccggc
     1261 aacgggatga gtaaagcggc aaacgggtga gtaacgcgtg ggtaatctac cctacggatc
     1321 gggataacct gccgaaaggt ggactaatac cggatgatgt tctcgcggtg attccgtggg
     1381 aaccaaaggg ggcgagcgta agcagctctc accgaaggag gagcctgcgt ccgattagct
     1441 agttggtggg gtaatggcct accaaggcga cgatcggtag ccggcctgag agggcgatcg
     1501 gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg
     1561 cgcaatgggc gaaagcctga cgcagcgacg ccgcgtgggt gatgaaggcc ttcgggtcgt
     1621 aaagccctgt ctggtggaac gaaacgcctg gcggccaata cctgccgggt ttgacggtac
     1681 caccgaagga agccccggct aactacgtgc cagcagccgc ggtaagacgt agggggcgag
     1741 cgttgttcgg atttactggg cgtaaagcgc gcgtaggcgg ccagataagt cgggtgtgaa
     1801 agccctcggc tcaaccgatg tttctgacag ggtactgcag gacttgagtg tggaagaggt
     1861 gagcggaact cccggtgtaa gggtgaaatc tgtagatatc gggaagaaca cccgtggcga
     1921 aggcggctct ctggactagt actgacgctg aggcgcgaaa gctaggggag cgaacgggat
     1981 tagatacccc ggtagtccta gccgtaaacg atgggcacta ggtgtgggag gtattgaccc
     2041 cttccgcgcc gaagctaacg cattaagtgc cccgcctggg gagtacggcc gcaaggctaa
     2101 aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttc aattcgacgc
     2161 aacgcgaaga accttaccag ggcttgacat cccacgaacc tgcctgaaag ggcggggtgc
     2221 ccgcaaggga gcgtggagac aggtggtgca tggctgtcgt cagctcgtgt cgtgagatgt
     2281 tgggttaagt c
//
LOCUS       DATMXI010000277         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_112972, whole genome shotgun sequence.
ACCESSION   DATMXI010000277 DATMXI010000000
VERSION     DATMXI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112972"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2603
                     /locus_tag="VN444_05210"
     CDS             <1..>2603
                     /locus_tag="VN444_05210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR21243.1"
                     /translation="AELKDVVERQGITKIYFRDATFTLNQERIARLCDLIVAAGWTLE
                     WVCETRVDCLGDALLEKMREAGCVGLLVGVETGDEQVMHLREGKKGLTIPMLAHLREK
                     THQLGIRLHFLLIVGLPQETRESLVATYDLIQRYKPDTIGVTIITPYPGTPLHKEGLR
                     EGWIDSQQWKDYGGHQIPMHTPNLARADMEAGKQFLEEGFALLQRRQVGGHSKPLEAM
                     AQQHYERLLRWAYRLDGPVAQLRELLAAAPKLPQASAQPSVNHAVRQAPSPARFALTV
                     VIPTYNRRAILRKTLLALMSQTFAPELFEVVVVDDGSSDDTVSMLRQFKAPFALRVLA
                     AEHGGANAARNLGIEAAQGRVVLITGDDMIPEPSFLEAHATFHERHPGDMDAMLGFID
                     WSPEIAVTPFMKFIVSAEGGQQFAFHEVRRGKADFRLFYTSNLSVKRDLLLNQPVLFD
                     QDFTYPAYDDVELGYRLSAQGLQLHYNAMAVTCHHHDITLAGFVQRQRKAGHMAVVLA
                     RKHPELSQTYLKIDDALKARNAFGETQVARIMQVAQELEKPDLQQLALIRSGAERFDR
                     TYLQRVLHPVYQTLLQSAYAWGICEAVEQGVATIPLATACVASKPRFKVSIIIPVFNK
                     LELTSQCLTTLASLPTVPEYEVIVVDNASSDGTAEFLATLGGDVQVIRNPENYGFAVA
                     CNQGAKAARGAFLLFLNNDTIPTEGWLNALVDEVERHPDVGVVGSKLLYEDGTIQHAG
                     VAFSRAVLLPYHIYRQFSADSPMVNRRREFQCVTGACMLVRREVFEQVGRFDEGFKNG
                     FEDVDLCLKIREQGWHIIYRPDSVVYHLESQTPGRKAHEKDNARRLLERWAHKWWIPD
                     EDALYFSDGLC"
BASE COUNT          536 a          754 c          757 g          556 t
ORIGIN      
        1 gctgagttga aagacgtcgt cgaacggcag gggatcacca agatctactt tcgcgatgcg
       61 accttcactc tcaatcagga gcgaatcgcc aggctctgcg acctcatcgt cgccgcaggc
      121 tggacgctcg agtgggtctg cgaaacgcgg gtcgattgtc tcggcgatgc cttgctggag
      181 aagatgcgcg aagccggctg tgtcggtctg ctggtcgggg tggagacagg cgatgaacag
      241 gtcatgcacc tgcgagaagg gaaaaagggc ctgacaatcc cgatgctggc ccatctgcgc
      301 gagaagactc accagcttgg catccggctt cactttctcc tcatcgtcgg ccttccgcaa
      361 gaaacccgcg aatcgctcgt cgccacctac gatttgattc agcgatacaa gccggacacg
      421 atcggcgtca cgattattac tccctaccct ggtacacccc ttcacaagga gggtcttcgc
      481 gagggctgga tcgattcaca gcaatggaaa gactatggcg gccaccagat tcccatgcat
      541 acgcccaacc ttgccagagc agacatggag gccggcaagc agtttttaga agaaggcttc
      601 gcgcttctgc aaagacggca agtcggcggg cattccaaac ccttggaggc tatggcgcaa
      661 caacattatg agcggttact gcggtgggcc tatcgcctcg acggaccggt tgcgcaactt
      721 cgggaactgc ttgcggcggc gccaaagttg ccgcaagcca gcgcacagcc ctctgtgaat
      781 cacgcggtac ggcaggctcc ctctcctgcg cgatttgcgc tcaccgtcgt gatcccgacc
      841 tataatcgac gggccatcct acgaaagact ctgctggcct tgatgtctca gacctttgcg
      901 ccggagctgt ttgaagtggt cgtcgtggat gacggatcct ccgatgacac ggtttcaatg
      961 ctccggcagt tcaaggctcc cttcgccctt cgcgtcctgg cggcagaaca tggcggcgcc
     1021 aatgcggcgc gcaatctcgg tattgaagct gctcaaggtc gcgtggtcct catcacgggc
     1081 gacgatatga ttccggagcc atcgttcctc gaggcgcatg cgacgtttca cgagcgccat
     1141 ccaggcgaca tggatgccat gctgggtttc atcgattggt ctcctgaaat tgcagtgacc
     1201 ccctttatga agttcatcgt gtcggcggag ggaggccagc agtttgcatt ccatgaggtg
     1261 cggagaggca aggccgactt tcgactcttc tacaccagca atctctcagt aaagcgggat
     1321 ctcttgctca atcagcccgt gctctttgat caagacttca cctatccagc ctatgacgat
     1381 gtggagttgg gctaccgttt gagcgcgcaa ggtctgcagt tgcattacaa cgccatggct
     1441 gtgacctgtc accaccatga catcacgctg gcggggttcg ttcaacgcca gcgcaaggcc
     1501 ggccacatgg cggtcgttct ggctcgtaaa catcctgaat tgagccagac ctatctgaaa
     1561 atcgacgatg ccctcaaggc ccgcaacgca tttggggaaa ctcaggttgc tcggatcatg
     1621 caggttgcgc aggagctgga aaagcccgat ctccagcagc tggcgttgat ccgctccggc
     1681 gctgaacggt ttgaccggac ctatctccaa cgagtgctcc atccggtcta tcaaacgctc
     1741 ttacagtccg cctacgcatg gggtatctgc gaggcggtcg aacagggtgt ggcgaccatc
     1801 ccgcttgcca ctgcatgtgt tgcgtcgaag cctcgcttca aggtttcgat catcatccct
     1861 gttttcaaca aactcgagct gaccagccaa tgtctcacga cactggcctc tctccccacg
     1921 gtgccggagt atgaggtgat cgtcgtggac aatgcttcaa gcgatggcac agcggagttt
     1981 ctcgctacgc tgggtggaga cgtccaggtc atccgcaatc ctgaaaacta tggctttgcc
     2041 gtcgcctgta accagggggc caaagcggcg cggggagcgt ttctcctgtt cttgaacaac
     2101 gacaccatcc cgaccgaggg ctggttgaat gccctggtgg atgaggtcga acgccaccct
     2161 gatgtcgggg tagtcggaag caagctgctg tacgaagacg gcaccattca acatgccggc
     2221 gtcgcattct cgcgggcggt attactgccc taccatatct atcgccagtt ttcggctgac
     2281 tcgccgatgg tcaatcggcg gcgtgaattc caatgtgtga ccggggcctg catgctggtc
     2341 cggcgcgagg tatttgaaca ggtcggccgg ttcgatgagg ggttcaagaa cggattcgaa
     2401 gacgtcgatc tctgtttgaa gattcgcgaa cagggttggc acatcatcta tcggccggac
     2461 agcgtcgtct atcacctgga aagtcaaacc cccggccgga aggcgcacga gaaagacaat
     2521 gcgcggcgat tgcttgaacg gtgggcccac aagtggtgga ttccggacga agatgccttg
     2581 tacttctcgg acggactctg ctg
//
LOCUS       DATMXI010000278         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_164333, whole genome shotgun sequence.
ACCESSION   DATMXI010000278 DATMXI010000000
VERSION     DATMXI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164333"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1391)
                     /gene="senA"
                     /locus_tag="VN444_05215"
     CDS             complement(75..1391)
                     /gene="senA"
                     /locus_tag="VN444_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HWR21244.1"
                     /translation="MPTTIPASQLACMVTDAHERTIGLVQDLAETQLVVPQTETVNPF
                     LWELGHTAFFYDVFLLPVLGSTKFLLEGAESFYDSFKIDHCDRWGLPLPSREATLDYK
                     NRVLDSVLKRLASDQPGAEETYLYLLSVLHEDMHEEAFTYMRQTLEYPAPGLSIAENG
                     SSAHGLGGGPWPGDVEIPGGVFQLGATPDLPFVFDNEKWAHPVDISPFRIARAPVTNA
                     QFAEFVGDRGYLQRKLWSPQGWIWRTKTGAQRPIYWNRGQNGWLRRHFDQLVPLEAHA
                     PVLHVNWYEADAYCHWAGRRLPTEAEWEMAASAEPAPGGTRITGHKRRYPWGDEPPTP
                     EHANLDSRMLGCVDVGAYAAGDSAFGCRQMIGNIWEWTASPFFPFPGFTVDSPYREYS
                     APWFGYRKVLRGGAWATRSRLVRNSFRNFFLPDRRDVFAGFRTCAL"
     gene            1591..>2531
                     /locus_tag="VN444_05220"
     CDS             1591..>2531
                     /locus_tag="VN444_05220"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWR21245.1"
                     /translation="MNTPYDLERLIRDLPKAELHVHIEGTLEPDLLLRIAGRNHVTLR
                     FATAEQLREAYQFHNLQSFLDIYYEATRALLSEQDFFDLTWAYLERAAQQHVRHVEIF
                     FDPQAHTGRGVPFETVIRGIHRALEEAIPRLSISSKLIMCFLRHLTADAAMQTLEASL
                     QFKDWIVAIGLDSSELNHPPNKFVEVFQRARVEGYLTVAHAGEEGPPDYIWQALDLLN
                     VSRIDHGVRCLEDPALVQRLARERIPLTVCPVSNVRLCVFKSLKEHNLKKLLDLGLCV
                     TVNSDDPAYFGGYITDNLLASQKALGLTRYDIYTLAR"
BASE COUNT          560 a          748 c          699 g          524 t
ORIGIN      
        1 gcctcatgtt cctgggtcag gctgaaatcc ataactcaca ccctctaccc tagaccctac
       61 accctgtgtt gatgttacag ggcgcaggta cgaaaccccg caaatacgtc acgtcggtcc
      121 ggcaggaaga aattgcgaaa cgagttgcgg accaggcgag atcgggtggc ccacgccccc
      181 cctctcaaga ccttgcgata gccgaaccaa ggcgccgaat attctctata tggcgaatcg
      241 accgtaaatc cggggaatgg aaagaagggg gaagcggtcc actcccagat attgccgatc
      301 atctggcggc agccaaaggc gctgtcaccg gccgcgtacg ctcccacatc aacgcagccc
      361 aacatacgtg aatccaggtt ggcgtgttcg ggcgtaggcg gctcatctcc ccaggggtag
      421 cgtcgcttgt gcccggtgat gcgtgtgccg ccaggggcgg gctccgcgct ggccgccatc
      481 tcccactctg cctcggtggg aagccgtctg cccgcccaat gacagtacgc atccgcctcg
      541 taccagttca cgtggaggac gggggcatgc gcttcgagtg gaacgagttg gtcgaaatgc
      601 ctgcgcagcc agccgttctg tccacggttc caatagatcg gccgttgcgc tcccgtcttg
      661 gtgcgccaga tccacccttg aggactccat aacttacgct gaaggtaacc ccgatccccg
      721 acgaactcgg cgaactgcgc gttggtcacc ggagctctgg cgatcctgaa tggagagatg
      781 tcgaccggat gagcccattt ctcattatcg aacacaaagg gcagatcagg cgtggcgccc
      841 aactgaaata ccccccctgg gatctcgacg tctccgggcc atgggccgcc gcccagcccg
      901 tgtgcgctcg acccattctc ggcgatacta agccctgggg cggggtattc cagggtctgc
      961 ctcatgtagg tgaatgcctc ctcgtgcatg tcttcgtgca gtacggagag cagatacagg
     1021 taggtctcct cagcgccggg ctggtcggaa gccagacgct tgaggacaga gtcgagtacc
     1081 ctgttcttgt aatcaagggt agcctctctc gaaggcaggg gcaggcccca gcgatcacag
     1141 tgatctatct tgaacgagtc gtaaaaactc tccgccccct ccagcaggaa cttggtggaa
     1201 ccaagaaccg gcaggagaaa gacatcataa aaaaaggccg tatgcccaag ctcccagagg
     1261 aaaggattga cagtctccgt ttggggaaca accagttgcg tctccgccag atcttgaacc
     1321 agcccgatgg ttcgctcgtg ggcgtcagta accatacagg caagctgaga cgcgggaatg
     1381 gtggttggca tctgatcctc cttagtgttc agtaagggcg ccgtactttt ttcacgcggg
     1441 aggccgcgct tgctgcggat tataagccca ccttcaggag cgggcaaggg ttctttgaat
     1501 caccggaagg cgctcaaaaa gtatggacgc aggagacagg tggtgtattc tctcaataag
     1561 gaacacccat cgggcgacga gaaaaacaag atgaataccc cctacgacct ggagcgactt
     1621 attcgtgatc ttccgaaggc agaattacat gtgcatattg agggtacatt agaaccagat
     1681 ctgctgctcc ggatcgccgg acgaaatcat gtcaccctgc gatttgccac agcagagcaa
     1741 ctgcgcgagg cctatcaatt ccacaacctc caatcgttcc tggatatcta ttacgaagca
     1801 acccgcgcac tgttgtccga gcaggatttc tttgatttga cctgggccta tcttgagcgt
     1861 gccgcgcaac agcatgttcg ccacgtggaa atcttcttcg atccccaggc ccacaccggt
     1921 cgaggcgtcc cgtttgaaac ggttatcagg ggcatccatc gcgccctcga agaggccatc
     1981 ccacgactca gcatctcatc aaagctgatt atgtgcttcc tccgccacct gaccgcggat
     2041 gcggctatgc agacgctcga ggcgtccctc caattcaagg actggattgt ggccatcgga
     2101 ttagattcct cagagctgaa tcatcccccc aacaagttcg ttgaggtgtt tcagcgggcc
     2161 agagtggaag ggtatttgac cgtcgcccac gcaggggaag aaggtccccc ggactatatc
     2221 tggcaggcgc tcgacctgct caacgtttcg cggatagacc atggcgtccg atgtcttgag
     2281 gatccagcgc tggtccaacg actggcccgg gaaaggattc ccctcaccgt gtgtcctgtc
     2341 tccaatgtca gactgtgtgt cttcaagtcg ttaaaggagc ataatctcaa gaagctgctc
     2401 gatctagggc tgtgcgttac cgtcaattct gatgatcccg cctactttgg cggttatatc
     2461 accgacaatc tcctggcttc acaaaaggct ctcggactta cccggtatga tatctacacg
     2521 ctcgccagga a
//
LOCUS       DATMXI010000279         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_322736, whole genome shotgun sequence.
ACCESSION   DATMXI010000279 DATMXI010000000
VERSION     DATMXI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322736"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VN444_05225"
     CDS             complement(<1..333)
                     /locus_tag="VN444_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21246.1"
                     /translation="MIRLQQVAKIYSLGKVEVRALDGVTLTIEQGEFVALVGPSGCGK
                     TTLLNLMAGIDRPTSGEVWLEGERLDSLSDNRLTRLRRTKVGIVYQLFNLLPTLTARE
                     NVALPLLLD"
     gene            611..1138
                     /gene="pal"
                     /locus_tag="VN444_05230"
     CDS             611..1138
                     /gene="pal"
                     /locus_tag="VN444_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWR21247.1"
                     /translation="MRRPWRLWPISSIGLLLTIAFLLSGCPMRAELAGEEEVKPSTPI
                     QEPRATGAPSAQQPAMASKESPLKDIFFDFDKSTMRDDAKPNLNEDLQWLNANPTEQI
                     TIEGHCDERGTAEYNLGLGERRAKAIRDYLVAAGIDARRIKTVSFGKERPFVLGHDEA
                     AWAWNRRAHFVVGER"
     gene            complement(1149..1310)
                     /locus_tag="VN444_05235"
                     /pseudo
     CDS             complement(1149..1310)
                     /locus_tag="VN444_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
     gene            complement(1445..1720)
                     /locus_tag="VN444_05240"
     CDS             complement(1445..1720)
                     /locus_tag="VN444_05240"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HWR21248.1"
                     /translation="MFRRSRILTSTSEERWRSERWATLKTPFIGFAILTRSHTKQQAP
                     YSTIVEVAVIFAGIFATMIPALAILEACRGTTLLTAISVGTVFMGAK"
     gene            1635..2636
                     /locus_tag="VN444_05245"
     CDS             1635..2636
                     /locus_tag="VN444_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWR21249.1"
                     /translation="MNGVFRVAHLSDLHLSSLVDVKIRDLLNKRALGYLSWRFRRRHE
                     HAPEILAALLHDLSGRALDHIVITGDLTQIGLPGEFQQARQWLETLGSPTDVTVVPGN
                     HDAYVRTPWADTFALWTPYMASDQGLQTGEQQVPDRTLFPSVRVRGHAVLIGVTSAHP
                     AAPFFATGSLGAAQMDRLGDILDLTRRQGLFRILLIHHPPMAAMVAWRKRLTDGAALM
                     ALLARHGVEMILHGHAHRSSVVYLPEVRGTIPIIGVPSASARGRKPEQRAGYHVYEVR
                     PSSHGWEVQTSVRSYSLRDHCFFAGDQRVIRVTDSATGSLYTTVHPHSHPTQHDQRA"
     gene            complement(2577..3644)
                     /gene="lptG"
                     /locus_tag="VN444_05250"
     CDS             complement(2577..3644)
                     /gene="lptG"
                     /locus_tag="VN444_05250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HWR21250.1"
                     /translation="MSTIDRHIWLRVIRSYALVMAVLLSVFSLMAFVTELDSIGRGTY
                     ALGDAVLNVLLTVPSRIIELAPATALLGSIIGLGELASSHELIAMQVLGTSPLRIGWS
                     VTVTGILLMIAVVGIQEWVAPSTDQLAYTRRIQAISNPEALHTKQGFWTRDGRQFIRV
                     HQVLPGRILSDVEIYEFDDHDRLRLVTWARRADPKDSDRWVLADVVQQTTEVEQVVTK
                     RLASLPWAGALTPAQVELLVLPVEILSRSTLGQYIAFLKRTGQDAERLEIRLWQQIAM
                     PLSTLMMVLVAIPFVLGPLRTATAGKRILHGSLVGAAFHLSSLSVAHLGAILHLNAAL
                     TVLSPLVVLGGVTVWVYRRVQ"
     gene            complement(3641..4705)
                     /gene="lptF"
                     /locus_tag="VN444_05255"
     CDS             complement(3641..4705)
                     /gene="lptF"
                     /locus_tag="VN444_05255"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWR21251.1"
                     /translation="MSILDRYLTREIAKPLFIFCGGLLLLFANFTAIRYLNQVADGVM
                     PAQSVLPLVLVRAVVALEILLPIALHLSIVVTLGRLYTDSEITALFGSGVSPARIIRI
                     VFAGSLVIAGIVAYLSIYARPWANETRYRLERQAVADIDLNDMKPGHFYENSSGTVLF
                     FQQRDRSSGRMQKVFIQSRSGDLVRLIWAEEGYYPGPVEMNAGQTLVCSGAQIYEISR
                     DQGRVRRATSDGLVLEWEKSHAPSEEYRRKAAQTWQLARSDSPKDIAEFQWRLSTPVA
                     TFLMGLLGFPLGRARARQGKYAKLFVSVLCYVIFYNLNMMARTWVAQGVVNTVPGIWW
                     VHLLLGVLVAGLLWRRITHP"
     gene            complement(5027..>5323)
                     /locus_tag="VN444_05260"
     CDS             complement(5027..>5323)
                     /locus_tag="VN444_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA-like NTP transferase domain containing
                     protein"
                     /protein_id="HWR21252.1"
                     /translation="VCGCNLYAFLTVRGRSLVEFWGQVEGLRKKPIRLISKLGWVPAL
                     GFFFGWLSLRQALARLSQRLDISIKVVMLPFPEAALDVDTVSDWELAQRIAGGK"
BASE COUNT         1206 a         1559 c         1475 g         1083 t
ORIGIN      
        1 gtctaataga aggggcaggg cgacgttctc tctggcggtg agtgtcggga gcagattgaa
       61 gagctggtag acgatcccga cttttgtccg acgaagccgt gtgaggcggt tgtcagaaag
      121 ggaatcgaga cgctctccct ctaaccacac ctcgccagag gtcggacggt cgattccggc
      181 catcagattg aggagagtgg ttttgccgca gccgctcggt ccaaccaatg cgacgaactc
      241 cccctgctca atggtcagcg taaccccatc cagagcccgc acctcaacct tgcccagcga
      301 atagatcttt gcaacctgct gcagcctgat catccacgcc ccctcaccct gatccaaccc
      361 attattacgt gtaccttccg ccgagtcaac gcactcgcaa agaccgggaa ttcgattgac
      421 aagcccacag gcgaatagta taggtacctc catgctgaaa tagcccgaaa ttacttcggt
      481 ccttacccgt ttccgtcttg cctgtcggca ggagactatt cgtcgtttgt gtggcgtgga
      541 tcgagtcaag gttgcggtga gcgttgcgag tcgctctgcc gcaaggaaca cttttgagga
      601 ggcgaaagca atgaggagac catggagact ttggcccatc tcaagcatag ggctgttgtt
      661 gactatcgcg tttctgctct caggctgccc gatgcgtgcg gaacttgccg gtgaagagga
      721 ggtcaaacca tctacaccga ttcaggagcc acgggcaaca ggggcaccct ccgcgcaaca
      781 accggcaatg gcaagcaagg agtcgccgct caaggacatc ttcttcgatt tcgacaaatc
      841 gaccatgcgc gacgatgcaa agccgaatct caacgaggat cttcagtggc tcaacgccaa
      901 tccgacggag cagatcacga tcgagggcca ctgcgacgaa cgcgggacgg cagaatataa
      961 tctgggcctg ggagaacgac gggccaaggc tatcagggac tacctggtag cggctggaat
     1021 cgatgccagg cgtatcaaga ccgtgagttt cgggaaggag cgtccattcg tcctgggtca
     1081 tgatgaggca gcctgggcat ggaaccgaag ggcgcacttt gtggtgggcg agcggtagca
     1141 ccacgaagct atgggcggaa gaagatgagg gtgaccagga gaaacagggg aaggaggatg
     1201 ccgacggtac acttcatgta cccaaagaaa gacggcatct tgaccccggc ctcttccgcg
     1261 atcgccttga ccatgaagtt ggggccgttg ccgatatagg tattcgctcc gtaactacaa
     1321 ggtgtttaga ctgaaggcta tgaatcatct cgcaaggtac gaatcgagtt aggtgttgag
     1381 gctgaaggcg atctcggttt ctacccctaa cagccttcag ccttcagtct atctacctga
     1441 gtagttactt cgctcccatg aagacggtgc caacagagat cgccgttagg agcgtcgtcc
     1501 ctctacatgc ctccaggatg gccagcgcag ggatcattgt cgcgaagatc cctgcgaaga
     1561 tcactgccac ctccacaatg gtggaatagg gagcttgttg ctttgtatgg ctcctcgtca
     1621 ggatggcaaa gccgatgaac ggggtcttca gggtcgccca cctctctgat ctccatctct
     1681 cttcactggt tgacgtcaag atccgcgacc tcctgaacaa acgcgcgctc ggttatctgt
     1741 cctggcggtt ccggcgtcgt catgagcacg cacctgagat cttagctgcc ctgcttcacg
     1801 atctgagtgg gcgggcgctg gaccatatcg tgattacggg tgatctgacc cagattggcc
     1861 tccccggtga atttcaacaa gcccgacaat ggctggagac ccttggttca ccgactgacg
     1921 tgaccgttgt cccgggtaac cacgatgcat acgtccgcac tccgtgggcg gacaccttcg
     1981 ccctgtggac accctatatg gcctccgatc agggattgca gaccggagaa cagcaggtgc
     2041 cagaccgtac actctttcca agcgtacgtg tgcgcgggca tgccgttctg attggagtga
     2101 cctccgcaca cccagccgct ccttttttcg ccacaggaag cctgggagct gcgcagatgg
     2161 acaggctcgg cgacatcctc gatctcaccc gccgacaggg cctgttcaga atcctcctca
     2221 ttcatcatcc ccctatggcg gccatggtcg cgtggcggaa gcgactcacc gatggggccg
     2281 cattgatggc cctgctcgct cggcacggcg tagagatgat tttgcacgga catgcccatc
     2341 gctcttccgt ggtgtacctt ccagaggtgc gaggcactat tcccattata ggagttcctt
     2401 ccgcctccgc gcgcggtcga aagccggagc agcgtgcggg gtatcatgtc tacgaggtgc
     2461 gtccgtcgtc tcacggatgg gaggtccaaa cgtcggtgcg cagctactcc ctgagggacc
     2521 actgtttttt tgcaggggat cagcgagtga taagggtgac ggactcggcg acgggctcac
     2581 tgtacacgac ggtacaccca cacagtcacc ccacccagca cgaccagcgg gcttaacaca
     2641 gtcagcgccg cgttcaaatg caagatggcc ccaagatggg ccacagaaag gctgctcagg
     2701 tgaaaggcag ccccgaccag cgatccgtga aggattcgct tgcccgccgt cgccgtccga
     2761 aggggaccga gcacaaaggg gattgcgacg agcaccatca tcagggtcga caggggcatc
     2821 gcgatctgct gccataacct gatctctaag cgttctgcgt cctggccggt tcttttcaga
     2881 aatgctatat actggccgag ggtcgaacgg gacagaatct cgacgggcag gacaagcaac
     2941 tccacctgtg ctggtgtcag tgctccagcc cagggaagac tggcaaggcg tttcgtcacg
     3001 acttgttcca cttcggttgt ttgttgcacc acatccgcga gcacccaccg atccgaatcc
     3061 ttgggatcgg ctcgccgtgc ccacgttacc agacgtaacc ggtcgtggtc gtcgaattcg
     3121 tagatctcga catcggagag aatgcgaccg ggcaacacct gatgcacccg gataaattgc
     3181 cgcccatcgc gcgtccagaa tccctgcttg gtgtgcaggg cttccggatt cgagatcgct
     3241 tggatacgac gcgtgtaggc gagttgatcc gtggacggag ccacccactc ctgaatccct
     3301 accaccgcaa tcatgaggag gatgccggtc acggtcaccg accatccaat acgcagcggc
     3361 gaggtgccta agacctgcat cgcgatgagt tcgtggctac tcgctaattc tccaaggccg
     3421 atgatgctcc ccagtagggc ggttgccggt gccagttcga ttatccggct cggtaccgtc
     3481 aagaggacgt tcaggaccgc gtcgcccaac gcgtaagtgc cgcgccctat cgagtccaac
     3541 tcggtaacga atgccatcag gctgaagaca gacaacagga cggccatcac aagggcatag
     3601 ctcctgatca ccctaagcca aatatggcga tcgattgtac tcatgggtgc gttatccgtc
     3661 tccacaacag gcctgcaacc aggaccccca agagaaggtg gacccaccag atacccggaa
     3721 cggtattcac aaccccctgc gcaacccatg tcctcgccat catgttcaga ttatagaaga
     3781 tgacataaca cagcaccgac acgaacaact tggcatactt accctggcgt gccctggcac
     3841 gaccgagagg aaagcccagc aatcccatca gaaaggtggc caccggggtt gagagtcgcc
     3901 attgaaactc cgcgatatcc ttgggcgaat cggagcgggc taactgccag gtttgggcgg
     3961 cttttcgcct gtactcctcc gatggggcat gcgatttctc ccactcgaga actaacccgt
     4021 cagatgtggc ccgtctcacc ctgccctgat ctcgagagat ctcatagatt tgtgctccag
     4081 agcagaccag agtctggccc gcattcatct ccaccggccc agggtaataa ccctcctccg
     4141 cccagatcag tcgaacaaga tcccccgacc gggactggat aaagaccttc tgcattcgac
     4201 cactgctccg atcgcgctgt tgaaagaaca agaccgtgcc ggaagagttt tcgtagaagt
     4261 gacccggttt catatcattt aggtcgatat ccgcaaccgc ttggcgttcc agacgatagc
     4321 gggtttcgtt ggcccatggt cgcgcataga tggacaaata ggccacgatc cccgctataa
     4381 cgagagaacc cgcgaatacg atgcgtatta tccgtgccgg actgacaccg gaaccaaaga
     4441 gggcggttat ttcggagtcg gtgtagagac gtcccagggt cacgacaatc gacaggtgca
     4501 gtgcgatcgg cagcaggatc tccagcgcga ccacagccct gaccaggacc agcggcagga
     4561 cgctttgggc gggcatgacg ccgtcggcta cctgattcag gtaacggatg gcggtaaaat
     4621 tggcaaatag cagtaacagc cctccgcaaa agatgaagag tggcttggcg atttcccggg
     4681 taagatatcg atcgagtatc gacatggtgt aactactcag gcgtttaggc tgaaggctga
     4741 aggttctgaa ttatatcgca agtcacgaat caagccgttc aagaccttct cggtttctat
     4801 gacttcttgt tcaacccgat aataccaaca ttgctacttc atcagccaac tcaaatacca
     4861 taagttttgt gtgctcacgc atgatttgtc cctaaaaacc ttcagccttc agcctatcta
     4921 ccttgtagtt acggtgcatc atcactatgc gcaggataag gacgacgcct gctcctgctt
     4981 gttgcgtcat ggccagacaa ctcggggcat ccggcctcgc ttgcgattac ttgcccccag
     5041 caattctctg agccagctcc caatcactca ccgtgtctac gtccaatgcc gcctcaggga
     5101 atgggagcat gacgaccttg atcgagatgt ccagacgctg cgagagtctg gcgagagcct
     5161 gacgaagaga gagccatccg aagaaaaacc ctagggcggg aacccatccc agtttcgaga
     5221 taagccggat cggtttcttg cgtagtcctt caacctgacc ccagaactcc accagggaac
     5281 ggcctcgtac ggtcagaaac gcgtacaggt tgcatccgca gac
//
LOCUS       DATMXI010000280         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_93241, whole genome shotgun sequence.
ACCESSION   DATMXI010000280 DATMXI010000000
VERSION     DATMXI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93241"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            685..2616
                     /locus_tag="VN444_05265"
     CDS             685..2616
                     /locus_tag="VN444_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR21253.1"
                     /translation="MDVVEKKKKKLKMSATPAVTDDAQTSLLASIVRSSDDAIIGKTP
                     DGVITSWNPAAVAMYGYTEEEAVGRPISILLPPDRQREMDEILARIRGGERVEHYETL
                     RKHKDGHLINVSLTVSPIYDASGRLIGASSIKRDITERKQAEERLLVTSQYARSLIEA
                     SLDPLVTISPEGKITDVNEATIKVTGISRDGLIGTDFSNYFTEPDNAREGYRQVFEKG
                     FVTDYPLTIRHKDGRLTDVLYNASVYRDVGGNVLGVFAAARDVTAQKEAEAKVAEQRA
                     REEQRARELERLAELERFQKLTVGRELKMVELKKEIEELKRENEQVRKGLVAERADRV
                     TPLTKPAQPDTNAGDYPRAMLNILEDFAGEKLRLEDTQRAVLNILEDFDVERSKTEAA
                     NLELREAFDSLRRAKDAAEAANRELEAFSYSVSHDLRAPLRAIDGFSQALLDDYHDLL
                     DEQGRDYLRRVRAATQRMARLIDDLLRLSRLTRGDLSIDLIDLSALARSVATELQKSH
                     PERQVAFTIANGLTARTDPHLLKVVIENLLDNAWKFTSKQEEAAIEFGVLPMNPTTAY
                     FVRDNGAGFDMTYADKLFATFQRLHTDKEFPGTGIGLSLVQRIVHRLGGQVWAKGEVG
                     RGATFYFTLKDNNEDGVKS"
     gene            2610..3062
                     /locus_tag="VN444_05270"
     CDS             2610..3062
                     /locus_tag="VN444_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21254.1"
                     /translation="MVTSGKTILLVEDNPDDVALTQRAFRNSKILNRLVVVQDGAEAL
                     DYLFATGPWEGLAPSDVPALILLDLKLPKIDGLEVLRRLRSDPRTKLIPVVILTSSVE
                     EQDVIRGYDLGCNSYIHKPVDFTQFVEAAQQLGMYWLVLNQPPLAVGT"
     gene            3064..4134
                     /locus_tag="VN444_05275"
     CDS             3064..4134
                     /locus_tag="VN444_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21255.1"
                     /translation="MEKPLRLLIVEDSEDDVVLVVRHLRQGGFAPVFERVETAESMAA
                     ALHRQPWDLVIADYSLPRFSGLAALALLKETEFDLPFIVVSGTIGEDLAVEAMKAGAH
                     DYILKGNLTRLLPAVQRELREAKMRQERRQATEDLRRSRDELRALTRHLESVREEERN
                     QIARDLHDETGGLLVAVYIVLDDCARDLPPDAQDRLKEVRALLDQVEEQLRRLSHELR
                     PAILEDLGVVPALQWLAEGVSKRTDLAITVEGDTKGRLPASVETVLYRCVQEGLTNAA
                     RHAKANSMRIQLQREAQQIRCTIRDNGIGFDLPAVLAQTGDRGLGLLGMRERLKALDG
                     ALQITTAPGQGTALLITIPLGA"
     gene            4136..>4421
                     /locus_tag="VN444_05280"
     CDS             4136..>4421
                     /locus_tag="VN444_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR21256.1"
                     /translation="MPIQVLLADDHPIVREGVKTLLEREGIDVVGEASDGREAVRLAQ
                     TLRPNVAVLDLSMPLLNGLLAGQEILHVSPRTKPILLTMHVEDHYVLHALR"
BASE COUNT          892 a         1375 c         1368 g          786 t
ORIGIN      
        1 agatctaccg caagacggcg tatgaatgcg gagccgacgc ctttctgacc aagaagaccg
       61 tgggagtcca gatccttccc acgattgtgg gcttctggtt tcctccgtca gtagactccg
      121 gcctttccgc gtttctaccg gcacctacct ggcctggaat cagcgcttga cctgctgctc
      181 catccccccc ggtaagcagc gcccaatagc tcgacagcgc gcatgacccg gattgtgagc
      241 cctcgctccc tcagaccctt ctcgatctgg acagggtgag ggcttcagac tgcgaggaca
      301 ttctgaagac ttttgacttg acttgctcgg ctcccgggga tacatttacc aaaacctacc
      361 agcctaccac caggtgagtt ctagcttgcc ctctgcaagg atgttgatgc gtctgagcca
      421 atggtagtga aatacggtaa cgcctgccac ttgccagcgc tatcgcgtgc ccgtagccgg
      481 tcctgtcctg agccttgtcg aagggcgcgt gaggtcgcat caaagtccgt ctccgcaccg
      541 attcgactca atccactctt caagatcaag aaatctgagt ctattgagtc gtcgagtctg
      601 ttgggtctgc cgagccgact caaaggactc aatcgactca atggacacaa tgacccagag
      661 ccagaggagg aaccgtaact gcctatggac gtcgttgaga agaaaaagaa gaagctgaag
      721 atgtcggcca cgccggcggt gacggacgat gcgcagacgt cactcctggc ctctatcgtt
      781 cgttcctccg acgacgccat catcgggaag acgcccgacg gggtaatcac gagctggaac
      841 cctgctgcgg tagcgatgta cggctatacg gaggaggagg ccgtcggcag gcccatctcc
      901 atcctcttgc cgcctgaccg gcagcgggag atggacgaga tcctcgcgag gatccgcggg
      961 ggcgagcggg tcgagcacta cgaaacgctc cgcaaacaca aagacggcca cctcattaat
     1021 gtgtcgctca ccgtctcgcc gatctatgac gcctccggcc gcctgattgg cgcgtcgtct
     1081 atcaaacggg acatcaccga acggaagcag gcggaggagc ggctgctggt gacatcccag
     1141 tacgcccgca gcctcatcga ggcgagcctg gaccccctgg tcaccatcag cccggaaggc
     1201 aagatcaccg acgtcaacga ggccacgatc aaagtgacgg gcatctcgcg ggacgggctg
     1261 atcggcaccg acttctcgaa ctattttacg gagccggata acgcgagaga ggggtatcgg
     1321 caggtcttcg agaaggggtt cgtcaccgac taccccctca ccatccgcca taaggacggg
     1381 cggctcacgg acgtcctcta caacgcctcg gtctaccgcg atgtgggcgg caacgtcctc
     1441 ggcgtcttcg ctgccgcccg cgacgtgacg gcccagaagg aggccgaggc aaaggtcgcc
     1501 gagcagcgcg cacgtgaaga gcagcgggcg cgcgagctcg agaggttggc ggagctcgag
     1561 cggttccaga agctgaccgt cggtcgcgag ctgaagatgg tcgagctgaa gaaagagatc
     1621 gaggagttga agcgggagaa cgaacaggtt cgcaagggcc tggtcgctga aagggctgac
     1681 cgggtgaccc cactgaccaa accggcgcag cccgacacca acgctggcga ttacccgcgg
     1741 gcaatgctca acatcctcga ggactttgcc ggcgagaagc tccgcctgga ggacacccag
     1801 cgcgcggtcc tcaacatcct cgaggacttc gacgtggagc gatccaagac cgaagccgcg
     1861 aacctggaac tgcgcgaggc atttgactcg ctgcgacggg ccaaggacgc ggccgaggcc
     1921 gccaaccgcg agctcgaggc cttcagctac tccgtgtccc acgaccttcg agcgccgctc
     1981 cgggccatcg acggcttcag ccaggcgctg ctggacgatt accacgacct gctcgatgaa
     2041 caaggccgcg actacctgag aagggtccgc gccgccaccc agcggatggc caggctcatc
     2101 gacgacctgt tgcggctgtc ccgcctgacc aggggcgatc tcagcatcga cctcatcgac
     2161 ctctcggccc tggccaggtc agtggcaacc gagttgcaga agtcgcaccc ggaacggcag
     2221 gttgcattca ccatcgcgaa tggacttacc gccaggaccg atccccatct gctcaaggtg
     2281 gttatcgaga acctcctgga caacgcctgg aagttcacga gcaagcagga agaggcggcg
     2341 atcgagttcg gcgtcctgcc gatgaatccg acgacggcct attttgttcg cgacaacggc
     2401 gccggcttcg acatgaccta tgcggacaag ctctttgcca ccttccagcg tctgcacacc
     2461 gacaaggagt tcccgggcac cggcatcggc ctgtcccttg tccagcgaat cgtccaccgc
     2521 ctgggcggcc aggtgtgggc gaaaggagag gtgggtcgcg gggccacctt ttatttcacc
     2581 ctgaaggata acaacgaaga cggagtcaaa tcgtgaccag cggaaagact attctacttg
     2641 tggaagataa tcccgatgac gtcgcgttaa cccaacgggc ctttagaaac agtaaaatcc
     2701 tcaaccgcct ggtcgtggta caggacgggg ctgaggcctt agactatctc tttgccaccg
     2761 gcccgtggga gggcctggcg ccttcagacg tgccggcgct gatcttgctt gacctcaagc
     2821 tccccaagat cgacggcctg gaggtgttgc ggcggctgcg ttcagatcct cggaccaagc
     2881 tgatcccggt ggtaatcctc acctcttccg tggaggagca agacgtcatt cgaggctacg
     2941 accttgggtg caacagctac attcacaagc cggtggactt tacccagttc gtcgaggcgg
     3001 cccagcagct tggcatgtac tggctggtgc tgaatcagcc gcctctcgct gtggggacct
     3061 agcgtggaaa agccgttgcg gctgctcatt gtcgaggact ctgaagatga cgtcgtgctc
     3121 gtggtgcggc atctgcggca gggcggcttc gctcctgtct ttgagcgggt cgagacggcc
     3181 gaatcgatgg cagccgccct tcacagacag ccctgggatc tggtcattgc cgattactcg
     3241 ctcccacgat tcagcggcct ggcggcgctc gcgcttctca aggagacgga gttcgacctg
     3301 ccgttcatcg tggtctcggg cacgatcggc gaggacctgg ccgtagaggc catgaaagcg
     3361 ggggctcacg actacatcct gaagggcaac ctgacacgac tgctgccggc tgttcagcgc
     3421 gaacttcggg aggccaagat gcggcaggaa cggcggcagg ccacggaaga tctgcggagg
     3481 tcgcgggacg agctgcgcgc cctgacgagg caccttgagt ccgtacgaga ggaggagcgg
     3541 aatcagatcg cccgcgatct gcatgatgag acaggcggac tcctcgtcgc cgtctatatc
     3601 gtactcgatg actgtgcccg cgacctgccg cctgacgctc aagatcgcct gaaggaggtc
     3661 agggcgctcc tcgatcaggt cgaggagcag ttgcggcgcc tctcgcatga gctccgtccc
     3721 gcgatcctcg aggaccttgg ggtcgtcccg gcccttcagt ggctggccga gggggtctcg
     3781 aaacggacgg atctcgcgat taccgtggaa ggcgacacaa agggcaggct cccggcctcg
     3841 gttgaaaccg ttctctaccg atgcgtccag gagggcctga ccaatgcggc gaggcacgcg
     3901 aaagccaaca gcatgaggat tcagcttcag cgggaggccc agcagatccg ttgcacgatc
     3961 agggacaacg ggatcggctt tgatttgccc gcggtcctgg cccaaacggg cgaccggggt
     4021 ctcggtttgc tcggtatgcg agaacgactc aaggccttag atggggcgct tcagatcacg
     4081 acagcccccg gccagggaac ggcgctgctc atcaccatcc ctctgggagc gtaagatgcc
     4141 gattcaggtt ctcctcgccg atgaccaccc gattgttcgc gagggggtca agaccctcct
     4201 ggagcgggaa gggatcgatg tggtcggcga ggcctcggac ggccgcgagg cagtgcggct
     4261 tgcccaaacg ctccgtccga acgtggccgt cctcgacctc tccatgccgc tgttgaacgg
     4321 cctattggcc ggccaggaga tcctgcacgt ctctccccgg acgaagccga tcttgctgac
     4381 catgcacgta gaggaccact acgtcctcca tgcgcttcgg a
//
LOCUS       DATMXI010000281         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_102937, whole genome shotgun sequence.
ACCESSION   DATMXI010000281 DATMXI010000000
VERSION     DATMXI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102937"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VN444_05285"
     CDS             complement(<1..645)
                     /locus_tag="VN444_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR21257.1"
                     /translation="MNDIVIEKLSKAYPSGWPGRPSFVALSGLSLTVGRGEIFGFLGP
                     NGAGKTTTLKILLGLVRATSGTAILLGKPAGDVETRRRVGFLPESPYFYDYLTAEEFL
                     GFYGQLAGLDRAGIAPRITTLLELVGLVEARTRQLRKFSKGMLQRIGLAQALIHDPQL
                     VILDEPMTGLDPVGRKQVRDLILGLRDRGKTVFFSTHILPDVEMICDRVGIVMKG"
     gene            complement(718..1611)
                     /locus_tag="VN444_05290"
     CDS             complement(718..1611)
                     /locus_tag="VN444_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21258.1"
                     /translation="MGTVPLKHLSIMAAVGLILFAGAAGLLSVLEQQRNASSRAEQLG
                     YLPKGEYLRLAVVGYRQLVADLIWLQAVQHIGAARDTQEGYSWTYHAVDVLTDLDPQF
                     VPPYQATGVLLGVLVGHHGEGIAILDKGMRNNPDVWQLPFLAGYISYYERCDPVAGGQ
                     YFRRAAQVPGSPAYLPRLAARMTVESGDPAAALEFLERFARSVQDERVREALVLRIKE
                     VSQEQDIRMLEEGVRLYYAQHQQFPSKLDDLLLRGIVRQLPVDLLGGEYQIDPMNGSV
                     SVSSRRERLQIDKKVACRVAG"
     gene            complement(1627..>2145)
                     /locus_tag="VN444_05295"
     CDS             complement(1627..>2145)
                     /locus_tag="VN444_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21259.1"
                     /translation="FERSGNEEAFRVAHAEYAEAMSLNPLDSRIPALMGQLHAFATRV
                     PRASSILEEQRVAQVRLALEGYSRAISLAPFSALYRYEQVKLHWLLGEKSNAERQAKE
                     VERLEPNFLPARMFMAQLALEENRIEEVKNQLREIQERQARYQHWQKNALEQLFLDVD
                     VEPIRVAIDKKG"
BASE COUNT          520 a          656 c          501 g          468 t
ORIGIN      
        1 acccttcatg acgataccaa cacgatcaca aatcatttcg acatcaggga gaatgtgtgt
       61 actgaaaaac accgtcttcc cccgatcacg caggccgaga atcaaatccc gcacttgctt
      121 ccgcccgaca ggatccagcc ccgtcatggg ctcatcaagg atgacaagtt ggggatcgtg
      181 gatcagtgcc tgggcgagcc cgatacgttg caacatccct ttcgagaact tgcggagttg
      241 gcgggttctc gcttcaacaa gccccacaag ctccagcagt gtagtgatgc gaggagcaat
      301 gcctgcgcga tccagccctg caagttgccc atagaacccc agaaactcct ctgccgtgag
      361 ataatcgtaa aaataaggcg actcaggaag aaatcccacc ctgcggcggg tttcaacgtc
      421 gccagccggc ttccccagta atattgcggt tccgcttgtc gcccgcacaa gacccagaag
      481 aatttttaag gttgtggttt tccccgcccc attcggacca agaaatccaa agatctctcc
      541 ccgaccgaca gtcaaagaca gccccgacaa tgcgacgaac gacggacgcc ccggccaacc
      601 tgaaggatag gccttgctca atttttcgat cacgatgtcg ttcacagctc tcctcctcat
      661 tacttcgtac cacccaggcc aggtcagcga accctggtag cgcttaacct caagggttta
      721 cccagcgacg cgacaggcca ccttcttatc gatctgaagc cgctcacgtc tagaggacac
      781 actaaccgac ccattcatag gatcgatctg atactcacca cccaatagat ctacagggag
      841 ttggcgaaca atccctcgta gcaggagatc atcaagtttt gaggggaact gctgatgctg
      901 agcgtagtac aggcgaacac cttcctcaag cattcggata tcctgctctt gcgaaacctc
      961 tttgatcctg agtacaagag cttcacgaac acgctcgtcc tgaacactgc gggcgaatcg
     1021 ttccaagaac tccaatgccg ctgcgggatc accactctcc accgtcatgc gggcggccag
     1081 ccttggcagg taggccggag agccgggcac ctgagcggcg cgccgaaaat attgccctcc
     1141 tgcaaccgga tcacaccgtt cgtagtacga gatatagccg gccaagaagg gaagctgcca
     1201 tacgtcgggg ttgttacgca tacccttatc gagaatcgcg attccttccc cgtggtgacc
     1261 gaccaatact ccgagtaaaa cacctgtcgc ctgatacgga ggtacgaact gcggatctag
     1321 atcagtaagc acatcaaccg catggtaggt ccaactatag ccctcttgcg tatctcgagc
     1381 tgcgccaatg tgctgcaccg cttgcagcca gatgagatct gcaacaagtt gccggtatcc
     1441 gaccactgct aatcgcaaat attctccctt tgggagataa cctaattgtt ccgctctgga
     1501 agatgcattc cgttgttgct caagaacgga caacagcccg gctgctccgg caaacaaaat
     1561 aagcccgact gccgccatga tcgacagatg cttcaaagga acagtcccca tttgccattc
     1621 atctcgctat ccctttttgt caattgcgac cctgataggc tcgacatcca catctagaaa
     1681 tagttgctcg agcgcattct tctgccagtg ttggtaccta gcctgacgtt cttgaatttc
     1741 gcggagttga ttcttgacct cctcgatccg gttctcctca agcgctaact gagccatgaa
     1801 catccgagca ggaagaaaat tgggctctaa tctctccact tctttcgctt gacgttccgc
     1861 gttactcttc tccccgagta accaatggag cttgacttgt tcatatcggt agagtgcaga
     1921 gaacggcgcg aggctaatcg ctctactgta gccttccagc gccagcctta cttgggcaac
     1981 tcgttgctcc tccaaaatgg aagaagcccg cggcaccctc gttgcgaacg catgcagctg
     2041 gcccataagc gccggaatcc gactatccaa cgggttgaga ctcatcgctt cggcatactc
     2101 cgcatgagca actctgaacg cttcctcatt gccactccgc tcaaa
//
LOCUS       DATMXI010000282         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_93961, whole genome shotgun sequence.
ACCESSION   DATMXI010000282 DATMXI010000000
VERSION     DATMXI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93961"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..>2156
                     /gene="secA"
                     /locus_tag="VN444_05300"
     CDS             188..>2156
                     /gene="secA"
                     /locus_tag="VN444_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWR21260.1"
                     /translation="MFMKLVSKIVGTKNERELKRIKPMVVAINELEPKLKALSDDDLR
                     GKTTEFRERIVQGATVDDLLIEAFAVVREAGRRVLNMRHFDVQMLGGIVLHEGKIAEM
                     ATGEGKTLVATLPVYLNALEGKGVHVVTVNDYLAKRDSQWMGGIYRFLGLSVGLIQHD
                     IDDATRKLSYGADVTYGTNNEYGFDYLRDNMKFSAADFVQRNLHYAIVDEVDSILIDE
                     ARTPLIISGPAEESTEKYYQIDQIIPRLKQGATIVGGKMYEAEAQVSGDYMVDEKAKS
                     VALTDSGMAKVEALLGVKNLYDPAHIEFVHHVQQALKAHVLFKRDVDYVVKDGEVIIV
                     DEFTGRLMPGRRWSDGLHQAVEAKERVKIERENQTLATITFQNYFRMYKKLAGMTGTA
                     DTEAAEFAQIYNLDVMVMPTNQPMVRTNYPDVIYKSVREKYDAVVEEIAELHKTGRPI
                     LVGTTSIEKNEKLSTLLKRKGIPHQVLNAKQHEREAEIVAQAGRFKAVTIATNMAGRG
                     TDILLGGSPKFLAAELLGRGEAPENGVDPHELASTLEEVRQMQHYGLLDLAVNAEEYA
                     AALAVVRKQTEAEHRQVVGQDGMHIIGTERHEARRIDNQLRGRAGRQGDPGSSRFYLS
                     LEDDLLRLFGSDRISTIMEKLGIDEGEPIEHS"
BASE COUNT          480 a          599 c          695 g          382 t
ORIGIN      
        1 ttgcgtaggg gcagggcttg ccctgcccct gatgggcgca gcaagcagcg cccctacgct
       61 cgacttcgtc agcctcgcaa taccccctgg cgcccacgtt acgtgccgct ttcgaattga
      121 aggcttccct cttctatggt aatatccgcc cggattcgtc agatattgga gaggagagaa
      181 ctcgaagatg ttcatgaagt tggtgtctaa gatcgttgga accaaaaacg agcgggagct
      241 caagcggatc aagccgatgg tcgtggcgat caatgagctg gagccaaaac tgaaggcgct
      301 ctccgatgac gaccttcgcg gcaagacgac cgagttcagg gagcggattg tacagggggc
      361 aaccgtcgac gatctcctca ttgaggcttt cgcggtcgtg cgggaggcag ggcgccgcgt
      421 gcttaatatg cgccacttcg atgtccagat gcttggcggc atcgtcctgc acgagggcaa
      481 gatcgccgag atggcgaccg gcgaaggcaa aaccctggtg gccacgctcc ctgtctacct
      541 gaatgccctg gagggaaagg gcgtccatgt ggtcaccgtg aacgactatc tggccaagcg
      601 ggatagtcag tggatgggcg ggatctaccg gttcctggga ctctccgtgg gcctgatcca
      661 gcacgacata gacgacgcga ctcgaaaact gtcctatggc gccgatgtga cctacgggac
      721 caacaacgaa tacggctttg attacctgcg cgataacatg aagttttccg cagcggactt
      781 cgtgcagcgg aatctgcact atgccatcgt ggatgaggtg gactcaatcc tgatcgatga
      841 ggcccgaacg ccgctcatca tctccgggcc ggcggaggaa tcgaccgaga agtactacca
      901 gatcgaccag atcatcccgc ggctgaagca gggggccacc attgtgggcg gcaagatgta
      961 cgaggcagag gctcaagtat ccggggatta tatggttgac gaaaaggcga agtcggtcgc
     1021 cctgaccgat agtggtatgg ccaaggtgga ggccctcctg ggggtgaaga acctctacga
     1081 tcctgcccac atagagttcg tccaccacgt ccagcaggca ctgaaggctc atgtcctctt
     1141 caagcgggac gtggactatg tggtcaagga tggtgaggtg ataatcgtcg atgagttcac
     1201 cggccgtctg atgcccggga ggcggtggag tgatgggctc catcaggcgg tagaggccaa
     1261 agagcgggtc aagatcgaac gggagaacca gaccctggcc accatcacct tccagaacta
     1321 cttccggatg tacaaaaagc tggcggggat gaccggaacg gccgatacgg aggcggcgga
     1381 attcgcccag atctacaatc tggacgtgat ggtgatgccg acgaaccagc cgatggtccg
     1441 gaccaactac ccggatgtca tctacaagag cgtacgggaa aagtacgacg ccgtcgtgga
     1501 ggagatcgcc gagttacaca agaccggacg gccgatcctg gtcgggacca cctcgatcga
     1561 aaagaacgaa aagctctcga ctctcctgaa acgaaagggg atccctcatc aggtgctgaa
     1621 cgccaagcag cacgagcgtg aggccgagat cgtggctcag gccggccgct ttaaggcggt
     1681 caccatcgcc accaatatgg ccggccgcgg gaccgatatc ctcctgggtg ggagtccgaa
     1741 gttcctggcg gcggagctgc tcggacgggg tgaggcgccg gagaatgggg tcgatccgca
     1801 tgagcttgcg agcacgctcg aggaagtccg tcagatgcag cactatggtc tgctggatct
     1861 ggcggtgaac gccgaggagt atgccgcggc gctggccgtc gttcgaaagc agaccgaggc
     1921 cgaacaccgg caggtggtag gccaggacgg catgcacatc atcgggaccg agcgccatga
     1981 ggcccgacgc atcgataatc agctccgggg gcgcgccggt cgtcagggcg accctggttc
     2041 atcccgcttc tacctctcgc tggaagacga tcttcttcgc ctgttcggat ccgaccgcat
     2101 cagcaccatc atggagaagc tggggattga cgagggggag ccgatcgagc acagca
//
LOCUS       DATMXI010000283         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_164558, whole genome shotgun sequence.
ACCESSION   DATMXI010000283 DATMXI010000000
VERSION     DATMXI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164558"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VN444_05305"
     CDS             <1..687
                     /locus_tag="VN444_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWR21261.1"
                     /translation="LLEVVIACHEDRAVRAVVLMGAGQAFCAGGDVREFIEHTDTIAL
                     YVKRLLMSLHSVITCICRMSKPVIAGVGGVAAGAGMGLAMACDLAVAAESARFTMAYT
                     KIGATPDASSSYFLPRLVGLRRAMELTFTNRVLTADEAKEWGLVNRVVPDAELVTAVD
                     AMANALATGPTLALGRAKRLLFMSDHASLETHMENEAQLIALSSQTADFREGVQAFAE
                     KRTPAFSGQE"
     gene            697..978
                     /gene="thiS"
                     /locus_tag="VN444_05310"
     CDS             697..978
                     /gene="thiS"
                     /locus_tag="VN444_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWR21262.1"
                     /translation="MTSRHTWAKVTTQKHSAISAVKGQRDMELTVNGKKLEAADGATI
                     TALLEELGINPLRVAVQLNQRIIKRELHENSVLQAGDALEIITFMAGGS"
     gene            1011..1787
                     /locus_tag="VN444_05315"
     CDS             1011..1787
                     /locus_tag="VN444_05315"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWR21263.1"
                     /translation="MENGDTLKFAGREFRSRLIVGTGKFPDHRTMQQAHEASGAEMVT
                     VAVRRVNLDRTQESLLDYIDTKRYALLPNTAGCYTADEAIKTAMLAREVGLSDMVKLE
                     VIGDQRTLFPDNEELLKATKVLVKEGFVVLPYTNDDPVMAKKLEDAGAAVVMPLAAPI
                     GSGLGIRNPHNIRIILEAARVPIIIDAGVGTASDASIAMELGCDGVLMNTAIACAKDP
                     VAMAAAMRHAMIAGRLAYLAGRIPKKLYANASSPLEGMIE"
     gene            1863..2549
                     /gene="thiE"
                     /locus_tag="VN444_05320"
     CDS             1863..2549
                     /gene="thiE"
                     /locus_tag="VN444_05320"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWR21264.1"
                     /translation="MDPRTFFSTQPSAFSLQPPSSWGLYVVTNRSMTNGRPLEVVVEA
                     ALAGGAKAIQLREKDLSTRDLYELVERLLPLVHGRGACLLINDRVDLALALPIDGVHL
                     SRTSLPPAETRALLGPARLIGVSCHSLEEAIEAEREGADFIVLGPLFPTPSKAAYGPP
                     IGLVRLKAVRQRVRLPIFGIGGITSSNTASVMAAGADGAAVISAIMTADDPADAVSTL
                     LQVVRSAARD"
BASE COUNT          502 a          727 c          838 g          509 t
ORIGIN      
        1 ctgctggagg tggtcatcgc ctgccatgag gaccgagcag ttcgcgcagt agtgctgatg
       61 ggcgccggcc aagccttttg cgccggcgga gatgtcaggg agttcatcga gcatactgac
      121 accatagccc tgtacgtaaa acggctgttg atgtctcttc atagtgtgat cacatgtatc
      181 tgtcggatgt caaagccggt catcgctggg gtgggcgggg tggccgcagg cgccgggatg
      241 ggcttggcca tggcctgcga tctggcggta gcggctgagt ccgctcgctt caccatggcc
      301 tatacgaaaa tcggcgccac cccagatgcc agttcgagtt attttcttcc ccgattggtg
      361 gggttgcggc gggcgatgga gttgaccttc accaaccggg ttctgacggc agacgaggcg
      421 aaagaatggg ggctggtgaa ccgggtggtg ccggacgctg agctcgttac cgctgtagac
      481 gccatggcga acgcgttggc caccggcccg acgcttgccc tcgggcgggc caagcggctc
      541 ctgttcatga gcgaccatgc aagcctggag acccacatgg agaacgaggc ccagctcatc
      601 gcgctgagta gccagactgc ggactttcgc gaaggggtac aggcctttgc cgagaagcgc
      661 acaccggcat tcagtgggca ggagtaagat ggtgcgttga caagtcgtca tacgtgggct
      721 aaggtcacaa cgcagaagca ctcagcgatc agcgcagtga aagggcagcg tgacatggaa
      781 ctcactgtca acgggaagaa gctcgaagcg gcagatggcg ccacgatcac cgcgctgctt
      841 gaggagttgg ggattaaccc gctcagggtt gcggttcagc tcaatcagcg gatcatcaag
      901 cgcgagctgc atgagaactc tgtgttgcaa gcaggggatg cgctggagat tatcaccttc
      961 atggctggcg ggtcctaatg aggagcgaga cagtgctgga gcagaatgtg atggagaacg
     1021 gcgataccct taaatttgcc ggtagggagt ttcggtcgcg gctcatcgtc ggtacgggca
     1081 agtttccaga ccatcgaacg atgcagcagg cacacgaggc ctccggcgcc gagatggtga
     1141 cagtagcggt tagaagggtg aatctggatc gaacccagga gtcgctcctg gactatatcg
     1201 acaccaagcg gtacgcgctc ctgcctaata ccgccggctg ctacactgcg gacgaggcga
     1261 tcaagaccgc gatgctggcc cgagaggtcg gcctctcgga catggttaag ctggaggtga
     1321 tcggcgatca gcggaccctc ttcccggaca acgaggagct gctgaaggcc accaaggtct
     1381 tggtcaagga gggcttcgtg gtcttgccct acaccaacga cgatccagtc atggcgaaga
     1441 agctggagga tgccggcgca gccgtcgtga tgccgcttgc cgccccgatc gggtccggcc
     1501 ttgggatacg aaatccgcac aacatcagga tcatcctcga ggcggcgcga gtcccgatca
     1561 ttatcgatgc gggagtgggt acggcttcgg atgcctctat cgctatggag ctgggatgcg
     1621 atggcgtcct gatgaacacg gcgatcgcct gcgccaaaga tcccgttgcg atggcggcag
     1681 cgatgcggca cgcgatgatt gccgggcgat tggcgtacct ggccggacgg ataccgaaga
     1741 agctctacgc caacgcctca agcccgctcg aaggcatgat cgagtaacgc cagtgtccag
     1801 ttgtgagtgt tgagtgttca gttgaaagac ctttgcgccg gcttcagata ggtacgtagc
     1861 ccgtggaccc tcggacattc ttctcaaccc agccttcagc cttcagcctt cagcctcctt
     1921 cttcctgggg tctgtacgtc gtaaccaacc gctccatgac gaacggtcgg ccccttgagg
     1981 tggtcgtcga ggccgcgctt gccggtgggg cgaaagccat tcagcttcgc gagaaggatc
     2041 tctcgacacg ggatctctat gaactggttg agcgactcct tcctcttgtg catgggcgag
     2101 gggcctgcct cctcatcaat gaccgagtcg acctggcgtt ggcccttccg attgacggtg
     2161 tccacctttc cagaacgagc cttccgcctg ccgagacacg ggctttgctg ggaccagcgc
     2221 ggctgatcgg cgtctcctgc cactctctcg aagaggcgat cgaggcggag agagaggggg
     2281 ccgacttcat cgtactcggt ccgctgttcc cgacgccatc caaggcagcg tacggaccgc
     2341 ccattggcct ggtgcggctt aaggctgtga gacagcgggt ccggctgccg atctttggca
     2401 ttggaggaat cacatcctca aataccgctt ccgtgatggc ggcaggtgcc gacggggcgg
     2461 ccgtgatctc cgcaatcatg acggcggacg atccagccga cgctgtgtct accctgctcc
     2521 aggtcgtccg ctctgcagcc agggattagg tgctgtccat gtggattgtg attgat
//
LOCUS       DATMXI010000284         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_202799, whole genome shotgun sequence.
ACCESSION   DATMXI010000284 DATMXI010000000
VERSION     DATMXI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202799"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VN444_05325"
     CDS             <1..1382
                     /locus_tag="VN444_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWR21265.1"
                     /translation="EMVLPICRCLLRDGHVIPLGAAEREAIVDRLNAFAGSALRVLAL
                     AYRELPEAAQLPLMEETERDLIFVGLIAMYDPPRPEVPDAVARCRRAGIKPIMITGDN
                     SHTALAIGRAIGMVRGDEALVLEGSRVERMRDEELKAALASPEILFARMTPTQKMRVV
                     TLLKEMGEVVAVTGDGVNDAPALKKADIGVAMGIAGTDVAKEAADIVLLDDNFATIVG
                     AVEEGRAVYENIRKFVTYILASNIPEIVPYLASVVFQIPLLLTVVQILGVDLGTDMLP
                     ALGLGAEPPDANTMDRPPRSREERLLNFPLLARSYLFLGPIEAAAAMSAGLWYLTTGG
                     WEWGIDLSEASPLYRQATTVAFAAIVICQVANVYVCRSQRASILSMGLFRNRLIVWGI
                     AVEISILGLIVYSQIGNRIFGTAAFDGRFWWSLLVFAALLLLAEEARKSIVRRLDPGR
                     GLRYQEGTA"
     gene            1379..1705
                     /locus_tag="VN444_05330"
     CDS             1379..1705
                     /locus_tag="VN444_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21266.1"
                     /translation="MTPKAAPSQKRQTEATEIEPSRDLGIEVERPLDAEDVALSLLDA
                     KVRAEEARADLSSADPHADLSVRLPRTVILRLSAEAANRNITPSELIKRALMQYLPLQ
                     KAGPGR"
     gene            1769..2332
                     /locus_tag="VN444_05335"
     CDS             1769..2332
                     /locus_tag="VN444_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR21267.1"
                     /translation="MLAGFPKQVTLQTGTSVTIRPMVKEDAEKLHAFFCRVPREDRLF
                     LRDDVSLREVIDSWTEELDYEKVLPLVAEVDDTIVADATLHRRKFGWTSHVGKVRLVV
                     DADYRGKGLGTLIVKELIDTARKAGLEILVAEIMGGQASSLSALKRLGFEKEAVFYNY
                     VKDQTGEEHNLVVMMKNLQIEPAMVPF"
BASE COUNT          578 a          766 c          902 g          541 t
ORIGIN      
        1 ccgagatggt gttgccgatc tgccgctgcc tccttcgtga cgggcacgtg atcccacttg
       61 gggcggccga gcgtgaggcg atcgttgacc ggctgaatgc gtttgccgga tcggccctgc
      121 gggtattggc cctggcgtac cgggagttgc cggaggccgc ccagctcccg ttgatggaag
      181 agacggagcg ggatctgatc tttgtcggtc tgatcgccat gtacgatccg ccaagaccgg
      241 aggtccccga tgcggtagcc cgctgcagga gggctgggat caagccgatc atgatcaccg
      301 gggataacag ccataccgcc ctggccatcg gacgggccat cgggatggtc cgcggggatg
      361 aggcgctggt tcttgagggt agtcgggtcg aacggatgcg ggacgaggag ctcaaggccg
      421 cactcgccag tccggagatt ctctttgcca ggatgacccc cacccagaag atgcgggttg
      481 tgacgctcct gaaggagatg ggagaggtag ttgctgtcac aggcgacggg gtcaatgacg
      541 ccccggcctt gaagaaggcc gacatcgggg tggcgatggg gatcgccggg accgatgtcg
      601 ccaaggaggc cgccgacatc gttctgctcg atgacaactt cgccaccatc gtcggcgccg
      661 tcgaagaggg gcgagcagtc tatgagaaca tccggaagtt cgtcacctac atccttgcga
      721 gcaacattcc ggagatcgtt ccctatctgg cctcggtggt gtttcagatc cccctcctgc
      781 tcacggtggt tcagatcctg ggcgtcgact taggcactga tatgctgccg gccctgggcc
      841 tcggggccga gccgccggat gcgaacacga tggatcgccc gccgaggtcc agagaggagc
      901 gccttttgaa tttcccgttg ctggccagat cgtacctttt tctcggaccg atcgaagcgg
      961 ctgcggccat gagcgccggg ctgtggtatc ttactaccgg tggatgggag tggggcatcg
     1021 acctgtcaga ggcaagcccg ctctacagac aagcgacgac ggtggcgttt gccgcgatcg
     1081 tcatctgcca ggtggcgaac gtctacgtgt gccggagcca gagggcatcg atcctctcta
     1141 tgggcctctt caggaatcga ctcatcgtct ggggtatcgc ggtcgagatt tcgattctcg
     1201 gcctgatcgt ctacagccag atcgggaacc ggatcttcgg gaccgccgcc ttcgatggaa
     1261 ggttctggtg gtctctgctt gtctttgcgg cgctgttgct ccttgcggaa gaggcgagga
     1321 agagcatcgt tcgccgcctc gatccaggga gaggacttcg ctatcaggag gggaccgcat
     1381 gacaccaaag gctgcgccat cacagaagcg acagacggaa gcgacagaga tcgaaccttc
     1441 gagggatctc gggatagagg tcgagaggcc gctcgatgcc gaggatgtag ccctgtcctt
     1501 gctcgatgca aaggtgcggg cagaagaggc gcgggcagat ctcagttcgg cagacccaca
     1561 tgcagacttg agcgtacgtc ttccgcgcac cgtgattttg cgcctctcgg cagaggctgc
     1621 caatcgtaac atcaccccgt cagagcttat caaacgggcc ctcatgcagt atctacccct
     1681 gcagaaggcc gggcccggca ggtaatgacg gacacacagg catgggccac gacagaagat
     1741 cggccctcac gataaaggag gctctctcat gctagccgga tttccaaagc aggtcacact
     1801 tcagaccgga acgagcgtca cgattcgccc tatggtcaaa gaggatgcgg agaaacttca
     1861 tgccttcttc tgcagggtgc cgcgcgagga tcgtctcttt ctgcgggacg atgtgtccct
     1921 ccgggaggtg atcgattcgt ggacggaaga gcttgattac gagaaggtcc ttccgctcgt
     1981 ggctgaggtg gacgacacca tcgtggctga tgccaccttg catcgccgaa aattcggttg
     2041 gaccagccat gtgggaaagg tacgcctggt cgtcgatgca gactaccggg ggaagggcct
     2101 cgggacgctc atagtcaagg agctgatcga caccgccagg aaggcaggcc ttgaaattct
     2161 cgtcgccgag atcatggggg gtcaggcgag tagtctgagc gccctcaagc ggttaggatt
     2221 cgaaaaggag gcagtcttct acaactatgt caaagatcaa acaggagagg aacacaacct
     2281 cgtggtcatg atgaagaacc tgcagattga gccggccatg gttccctttt aatgcggaag
     2341 gtaactactt aggtagatag actgtaggct tttagactgt aggaacgtgc catgcgagac
     2401 catgccaagc tcagagcgtt tgaactggcc gacgaagtgc accttgattc ggtccgtgcg
     2461 caacggctaa cggccttcag cctacagcct aaacacctga gtagttctgc cgaaggctga
     2521 tcgacccgcg ctgcagggcg acagcgaaga ttttggactg tgaggcatga cctatgaagc
     2581 tggtgatcag gctgaagcca cccaagagac gggcccccca tcggcctaca aaggtagagc
     2641 cacagagggt tcgctacgat cggaagaggg ccaagcggtc ggttcgggag gagttgaagg
     2701 gagatgggtg agacgatggc cagtccattg aaatctgata cgctccgtaa gatctgccct
     2761 ccggagagcc tccccttcga agggact
//
LOCUS       DATMXI010000285         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_281660, whole genome shotgun sequence.
ACCESSION   DATMXI010000285 DATMXI010000000
VERSION     DATMXI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281660"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VN444_05340"
     CDS             <1..698
                     /locus_tag="VN444_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWR21268.1"
                     /translation="VAQAMDTTGLMNSSLTEIFSNAADGVLGVDQDHMITLWNEAAER
                     LVGYAAAEVMGRPCSEVWAGRDRIGCRLCGADCSPITSARKEEPVEGREIMIHTKAGR
                     PLWLHVSTIVVPTGAPNLFTMVHFFHDVTRQVETEVLLGKVQSLLGGEGAIHDGGRMS
                     PLQSASPLKVLTPREQEVLRFIARGESAKGIAKQLCISTTTARNHTQKILAKLGLHTK
                     LEAVAVAFRYKHF"
     gene            838..1557
                     /locus_tag="VN444_05345"
     CDS             838..1557
                     /locus_tag="VN444_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWR21269.1"
                     /translation="MNAVSLVVQAGLVAKAVLLLLLGFSLMSWTLIFMKFGVFRRGQR
                     ESTQFLHIFRSAKNLTTVFEESKRFAKSPIVSVFQEGYRELSQLVKGSQGPANQWPAA
                     NEPRATVAEIPIHKEPLELISRTLRHASMKEVAQQERHLIFLATTGNVAPFVGLFGTV
                     WGIMDAFASIGQAGSANLGAVAPGVAEALITTAAGLGAAIPAVIAYNYFLNRVRRQAT
                     EMEMFGLEFLTLAERILGRSR"
     gene            1560..2000
                     /locus_tag="VN444_05350"
     CDS             1560..2000
                     /locus_tag="VN444_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWR21270.1"
                     /translation="MMGSSGTGSGERPGGSALSEINVTPFVDVVLVLLVIFLITAPMM
                     LRGIDVKVPKTEAKNVGPEERLMLTVTKEKAVYLDDQPITLARLEGVLVGLRQRNAKA
                     AVFLRADEGVAYGVVVKVMDAVKKAGIERLGMVTEPIPPVAKGR"
     gene            2002..>2435
                     /locus_tag="VN444_05355"
     CDS             2002..>2435
                     /locus_tag="VN444_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21271.1"
                     /translation="MAVGAIGRRLPLPEVSSSCGLSFLLHGLLALAVLYVPQWLHGKP
                     FKVPVSYEVTLVSPKETDSKQKPDSPLRIGQKVATATAKAAAQPRDVLMLPSLPKPSI
                     SGAAVRPHAAPTRTEEMALSGRHRPSERQPVVPMVAPPAPPK"
BASE COUNT          490 a          670 c          765 g          510 t
ORIGIN      
        1 gcgtggcaca ggcaatggat acgacaggtc tgatgaatag ttcactgacg gagatctttt
       61 cgaacgcggc agatggggtt ctcggtgtcg atcaggacca catgatcacc ctgtggaacg
      121 aggcggctga gcgcctcgtg gggtacgcgg ccgcagaggt tatggggcga ccctgttctg
      181 aggtctgggc tgggagagac cgaatagggt gccgactgtg cggagctgac tgctccccga
      241 tcacctcagc caggaaagag gagccggtcg aggggcgtga gatcatgatc cataccaagg
      301 ccgggcgccc cctctggctc cacgtcagca ccatcgtcgt tcctaccggt gcgccgaacc
      361 tcttcaccat ggtgcacttc tttcacgacg tgacccggca ggtagagacc gaggtcctcc
      421 tcggcaaggt gcaatcgttg ctggggggtg agggagccat ccatgatggt ggtagaatga
      481 gccccctaca gagcgcctct cccttgaagg tgctaacgcc ccgagagcag gaggtcctcc
      541 ggttcatcgc ccgtggcgag agtgccaagg ggattgccaa acagctgtgc atcagcacca
      601 ccacggctcg caaccatacc cagaagattc tcgccaaact tggcctccac actaaacttg
      661 aggcggtggc ggtcgccttc cgctacaagc atttctagcc tcgtttcggc ttatgtcttc
      721 cagcgttgac gatgcggctt ccccctactt gaagggcttg ctccctcaat cacgccggtt
      781 gaatgatctt gaccatgata ggcggtgatg gtataagcac aacttaagga ctatgagatg
      841 aatgccgtaa gcctcgttgt acaagcaggg ctcgttgcga aggcggtgct gctcctcttg
      901 ttgggttttt ccttgatgag ctggacgctg atcttcatga agttcggagt gttccggcga
      961 ggccaacggg aatcgactca gttcctccac atctttcgat cagccaagaa cctcacgacc
     1021 gtctttgaag aatcgaagcg atttgcaaaa agccctattg tgagtgtgtt tcaggagggg
     1081 taccgagagc tcagccagtt ggtgaaaggc agccaaggtc cagccaatca atggcctgcc
     1141 gccaatgagc caagggcaac ggtagccgaa atacccattc acaaggagcc actcgagctc
     1201 atcagccgga ccctccgcca tgcgagcatg aaggaggttg cgcaacaaga gcgccatctg
     1261 atcttcctgg cgacgacagg caacgtggcg ccgttcgtcg gtctctttgg gacggtatgg
     1321 gggatcatgg atgcctttgc cagtattggt caggcgggtt ccgcgaactt gggtgctgtg
     1381 gcgcctgggg tcgcagaagc cctgatcact acggcggctg gactgggggc ggccatccct
     1441 gcggtgattg cctataacta tttcttgaat cgagtcagaa ggcaggcaac ggagatggaa
     1501 atgttcggcc tggagtttct caccttggcc gaacggattc tgggaaggag ccgctgacga
     1561 tgatgggctc atcgggcaca ggttcgggtg aacgaccagg gggtagcgcg ctctctgaga
     1621 tcaacgtcac gccgtttgtc gatgtggtct tggtcctcct cgtcatcttc cttatcactg
     1681 caccgatgat gctcaggggg atcgatgtca aggtgccgaa gaccgaggcc aagaatgtcg
     1741 ggcccgagga gcggctgatg ctgacggtca ccaaggagaa ggccgtctac ctggatgatc
     1801 agcctatcac ccttgcccgg ctggaggggg tgcttgtggg gcttcgacag cgcaatgcaa
     1861 aggctgccgt attccttcga gcggatgaag gggtggctta cggtgttgtc gtgaaggtta
     1921 tggatgcggt gaagaaggcc ggcattgaac ggttggggat ggtgaccgaa ccgattcctc
     1981 ctgtggcgaa gggacggtag cgtggcggtg ggagcgatcg ggcggcgcct ccctttgccg
     2041 gaagtgtcgt cctcgtgtgg tctctcgttt ttactccatg gacttctggc gcttgcggtg
     2101 ttgtatgtcc cacagtggct tcacggtaag ccattcaagg tgccggtcag ctacgaagtg
     2161 acgctcgtat ctccaaagga gacagacagc aagcagaagc cggattctcc tctccggata
     2221 gggcagaagg tcgcaaccgc aacggcgaaa gccgccgctc agccgcggga tgttctcatg
     2281 ctaccatcgc ttcccaaacc gagtatttcg ggggcggcgg tccgacccca cgccgcccca
     2341 acgcggaccg aggagatggc tttgtcaggt aggcaccggc catccgagcg ccagcctgtt
     2401 gtacctatgg tagcaccccc tgctcccccg aagat
//
LOCUS       DATMXI010000286         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_44631, whole genome shotgun sequence.
ACCESSION   DATMXI010000286 DATMXI010000000
VERSION     DATMXI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44631"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /gene="bamA"
                     /locus_tag="VN444_05360"
     CDS             complement(<1..2006)
                     /gene="bamA"
                     /locus_tag="VN444_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWR21272.1"
                     /translation="MQAFANSVKVLVASSLVFFLALACGEAYGQEQAQVKQLDIKGSR
                     KIDEATIRLKLKTRVGELFSLEKIREDVKTVYRLGFYDDVAADAEVFEGGLKITFILT
                     EKPTIREVKIRGNKQIATDKIKEKLALAEGGVFSPQAVTANAGKVRLFYEEEGYYQAK
                     VLPQIDKTAEGDVAVTFQIDEGGKFEISTIRILGAKGLSEQEIKAHMATRELFLFFFF
                     GTLKRDELQRDLDRIKAFYLDNGYLDIKVGEPEIRVIETRQKLDISMRVEEGPRYRVG
                     EVRVTGNTVFSTEEVLKPLQIARQGVFSREVLQRDMLTLTDRYSERGYLFADIAPTIN
                     TDRENHIVDVGLEIGEGKQAFLERIEISGNTKTRDKVIRREIPLIEGDLYNSRLLARG
                     RQNLTNLGYFEDVKVETRRGTSEDRVDVDVTVKEKPTGSFSIGGGFSSIDGMMGSGSI
                     TQDNFLGLGQRVSLSAQLGVRATRFVMNFFDPHILDTETSLDLSLFNQRLLFNEQVGY
                     NQDIKGGSIALGRRLYKQLFGTLGYRYEVNRIFDVNENAPDAIKDQKGTNVTGRVSTG
                     LSLDLRDNPRDPTRGFSGAATYQIAGTFLGGENKFQRLNLDLGYYHPLIWKLVGHVRG
                     NLIVAEPYGKKDLPVQELIYLGGTNTVRGFKTFHLSPIDPATGER"
     gene            complement(2152..4581)
                     /locus_tag="VN444_05365"
     CDS             complement(2152..4581)
                     /locus_tag="VN444_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWR21273.1"
                     /translation="MFERFTERARKVIILAREEAIRLGHNFVGTEHLLLGLIREGDGL
                     AVAILKKLNVNISSVKGEIEKIVSVGLEFSPAGEIPFTPQAKKVLEYAISEARSLGHN
                     YIGTEHLLLGLIREGEGIASLVLRDFGVSVASAKAQAQELLGEQASKPTSSTRTPALD
                     EFGVDLTAMARQDRLDPVIGRETEIERVIQILSRRTKNNPVLIGEAGVGKTAIVEGLA
                     QRIVASNVPETLLRKRVVQLDLAGMVAGTKYRGQFEERLKAVVREIQQTQSIILFIDE
                     LHTLVGAGAAEGAIDAASMLKPALARGELQCIGATTLDEYRRHIEKDRALERRFQAVQ
                     VGPPSVEETIHILREIKDRYEAHHCAVITDEAVAAAARLSQRYIADRFLPDKAIDVID
                     EAGSRARLKTLMLPQDLREMENEVERLRAQKEDAIRTQAFEVAARLRDSERKLRTELE
                     EKKARWKESRAKEKTVVTAEEVAYIVSKWTGIPLYQIEEEETAKLIRMEQDLAKRVVG
                     QTEAIESVSRAIRRSRAGIKSPSRPVGSFIFLGPTGVGKTELAKALAEFLFGTEDALI
                     RVDMSEYMERFSTSRLIGAPPGYIGYDDSSQLTEKVRRRPFSVILLDEIEKAHPEVFN
                     LLLQIFEDGRLTGSYGRIVDFKNTILIMTSNIGARQIGLHAAMGFAKGGDETVYDKMK
                     DTVLGELKRAFNPELLNRIDEVIVFHQLSKNELCKIVDLMLAHLQLQLAERKISLAVD
                     ESAKDFLINRGFDPTLGARPLRRAIQRYVEDPLAEEVLKGRFTEGGILRVTLEGDALT
                     FEEVSLLEASK"
BASE COUNT          968 a         1498 c         1261 g         1071 t
ORIGIN      
        1 cgctcccctg ttgcaggatc gatagggctc agatggaagg tcttgaaccc ccgaacggtg
       61 ttggtaccgc cgaggtagat cagttcctgg accggcaggt ccttcttgcc gtagggctcc
      121 gccacaatga ggttgccacg gacatgaccg accagcttcc agatcagcgg gtggtagtaa
      181 cccagatcga ggttgaggcg ctgaaacttg ttttccccac ccagaaaggt ccctgcaatc
      241 tgatatgttg cagccccgct gaagcctctg gtcggatcac gggggttgtc cctgaggtct
      301 aacgagaggc cagtagagac ccgcccagtc acattcgtcc ctttctggtc cttgatcgca
      361 tccggggcgt tctcgttaac atcaaagatc cggttcacct cataccggta cccaagggtc
      421 ccgaacaact gcttatagag cctccggccg agggcgatcg agccgccctt gatatcctga
      481 ttatagccga cctgctcgtt gaagagcaga cgctgattga acagcgagag gtcaagcgat
      541 gtctcagtgt ccagaatgtg gggatcaaag aagttcatga caaaccgagt agccctgacg
      601 ccaagctgcg ctgagagcga gacccgttgc cccagtccga gaaagttatc ctgggtgatg
      661 gagccagaac ccatcatccc atcaatcgaa ctgaagccgc ctccgatact aaaggagccg
      721 gtaggcttct ccttcaccgt cacatcgacg tccacccgat cctcggacgt gccacgacgg
      781 gtctcgacct tgacatcctc gaagtagcca aggttggtca gattctggcg accgcgcgcc
      841 agcagacggc tattatacaa gtcgccctcg atcagaggga tctcccttcg gatcactttg
      901 tctcgggtct ttgtgttgcc tgagatttcg atccgctcca ggaaggcctg tttcccttca
      961 ccgatttcca ggcccacatc cacaatatgg ttctctcgat cagtgttaat ggtcggggcg
     1021 atatcggcga agagatagcc acgctccgaa taccggtcgg tgagggtcag catgtcccgc
     1081 tggagtacct cccggctaaa gacgccctgt ctggcgatct gaagcggctt caacacctcc
     1141 tcggtggaga agacggtgtt gcccgtcacc cgtacctctc cgacccggta ccgcgggccc
     1201 tcttccacgc gcatgctgat atcgagcttc tgccttgtct cgatcacccg aatctccggc
     1261 tcccccactt taatgtcaag atagccgtta tccaggtaga aggccttgat ccgatcgaga
     1321 tcgcgttgca gctcatcacg tttcagcgtc ccaaaaaaga agaacaggaa tagttccctg
     1381 gtggccatgt gcgccttgat ctcctgttcg cttaatcctt tggctccaag gatccgaatg
     1441 gtagaaatct caaactttcc cccttcatcg atctggaagg tgaccgcgac atccccctcc
     1501 gcagtcttgt ctatttgcgg gagaaccttg gcctggtaat acccctcttc ttcataaaac
     1561 aaccggacct tgcctgcgtt cgccgtcacg gcctgcgggc tgaacactcc cccctcggcc
     1621 agtgcgagct tctccttgat cttgtcggtg gcaatctgct tattgcctcg gatctttact
     1681 tcccggatgg tcggcttttc ggtgaggatg aaggtaatct tcaggccccc ttcaaacact
     1741 tcggcatccg cagcgacatc atcgtagaag cccaaccggt agacggtctt gacatcctcc
     1801 ctgatctttt ccagagagaa cagctctcca accctggtct tcagcttcag ccggatcgtg
     1861 gcctcatcga tctttcggct tcccttgata tcgagctgtt tgacctgtgc ctgttcctgg
     1921 ccataagctt caccacacgc caatgccagg aagaaaacga gagaactggc gacaagaact
     1981 tttacgctat tcgcgaaggc ctgcaccgat cgctatcgct cctcgattct ggacgagatg
     2041 cctgcactca aatgaaattc agtatttata ctacctttgc cgcacaagca caaggagaaa
     2101 ctcaccagga cggatcgtaa gtgaagagat atggggtgga aagaggtaag gctatttaga
     2161 ggcttcgagg aggctgacct cttcgaatgt caaggcatca ccctcgagtg taaccctgag
     2221 gatgcctcct tctgtaaatc gccccttcaa gacctcttca gctaatggat cctccacata
     2281 gcgctgaatg gcacggcgga ggggcctggc accaagggtt gggtcaaaac ctcggttgat
     2341 gagaaaatct ttcgcgctct cgtcgaccgc aagcgagatc ttgcgctccg caagctgaag
     2401 ctggagatga gcgagcatca gatctacgat cttgcagagt tcgtttttgc tcaactggtg
     2461 gaagacgatc acttcgtcga tccggttgag cagttccgga ttgaaggccc gtttcagctc
     2521 gcccaggaca gtatccttca tcttatcgta gaccgtctcg tcaccgccct tggcgaagcc
     2581 cattgcggca tggaggccga tctgacgggc gccgatgttg ctcgtcatga tcagtatagt
     2641 attcttgaag tcaacgatgc gcccatagct gccagtaaga cggccatcct caaagatctg
     2701 gaggagcaga ttgaatacct ccggatgagc tttttctatc tcatcgagga gaatgacgga
     2761 gaatggccgg cgccggacct tctcagtgag ctggctcgaa tcgtcgtacc cgatataccc
     2821 cggaggggcg ccgatgagac gggatgtgga gaagcgctcc atgtactccg acatatcgac
     2881 gcggatcagc gcgtcctccg ttccgaacag gaattccgcc agcgccttgg ccagctcggt
     2941 cttccccact ccggttggtc caaggaagat gaacgatccg accggacggc tcgggctctt
     3001 aatcccggcc cgcgaacggc gaatcgcacg actcacgctc tcgatggcct cagtctgccc
     3061 caccacccgc ttggccagat cctgctccat ccggatcagc ttggctgttt cctcttcctc
     3121 aatctggtac agcggaatac ctgtccactt ggagacgatg taggcgacct cctcggctgt
     3181 caccacggtc ttctccttcg ccctggactc cttccagcga gcctttttct cttccaactc
     3241 ggttcggagc ttgcgctccg aatctcggag cctagccgca acctcaaagg cctgggtccg
     3301 aatcgcgtct tccttctggg cccggaggcg ctcgacttcg ttctccatct cccggagatc
     3361 ctgtggcaac atcagggttt tcagccgcgc tcgcgagccg gcctcgtcaa tgacatcaat
     3421 agccttatca ggcaaaaagc ggtcggcgat gtagcgctga gacagacgcg cagcggccgc
     3481 gacggcctcg tcagtgatga ccgcgcagtg atgcgcctcg tagcgatcct taatctcccg
     3541 gaggatgtga atcgtctcct ccacgctcgg cggcccgacc tggactgcct gaaaccgtcg
     3601 ctccagcgct cggtctttct caatatggcg gcgatactcg tcgagggtgg tagcaccgat
     3661 gcactgcagc tccccgcgcg cgagtgccgg cttcaacata ctggctgcgt cgatcgcgcc
     3721 ctctgcggca ccggcgccca ctaacgtgtg caactcatcg atgaacagga tgatgctctg
     3781 tgtttgctgt atctccctga cgacggcctt caaccgctcc tcgaactgac cgcgatactt
     3841 ggtcccggcc accatgccgg cgaggtcaag ctggacgacc cgcttccgga gcagggtctc
     3901 cggtacgtta ctggccacaa tgcgttgcgc aagcccttcc acgatggcag tctttcccac
     3961 gccggcctcc ccgatcagga ccggattatt ctttgtccgg cgcgagagaa tctggatgac
     4021 ccgctcgatc tccgtctcac ggccgatgac gggatccagg cgatcctggc gcgccatcgc
     4081 ggtcaggtct acgccgaact catccagcgc gggtgtcctg gttgacgacg tgggcttcga
     4141 ggcttgctca cccagcagct cttgggcctg cgccttcgca gaggctacgc tgacgccgaa
     4201 atccctcaag accaaggagg cgatcccttc gccctcgcgg atcagtccga gcagcaggtg
     4261 ctcagtgccg atatagttgt gtccgagcga tctcgcttca gagatggcat attccagaac
     4321 cttcttcgcc tgcggggtaa aggggatctc gccggccggg ctgaactcta agccgactga
     4381 gacgatcttc tcgatctctc ccttcaccga ggagatattc acgttcagct tcttgagaat
     4441 cgcgaccgcg agtccgtcgc cctcgcggat cagtccgagc agcaggtgct cagtgccgac
     4501 gaagttgtgt ccgaggcgga tcgcctcctc ccgggccagg ataatcacct tgcgggctcg
     4561 ctctgtaaat cgctcgaaca tctcctcgct cccctctctt cagcacccta gaatcactag
     4621 cgttttcagt atagccgatc ctttcagaat ttcaagcccc ctctgagcct gtcctgcaaa
     4681 ccccatgtcg cctacttcga ccttgctcac cacagcctcc gacgagcccg tcctgagtac
     4741 agtcgaagaa ctcatgcttc gacaagctca gcggaacggg aaaatatcag tgcttccg
//
LOCUS       DATMXI010000287         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_221112, whole genome shotgun sequence.
ACCESSION   DATMXI010000287 DATMXI010000000
VERSION     DATMXI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221112"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..430)
                     /locus_tag="VN444_05370"
     CDS             complement(92..430)
                     /locus_tag="VN444_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21274.1"
                     /translation="MKLNRRLTALLALCLLVQMTVASWARAEETSLGADVSAGAVSVI
                     ATVITLPLKLVACAATVALGGTVYGLTMGTSEIIREELAAGTNQTCGGRYYVSPQQVK
                     QLVREPEQQR"
     gene            complement(580..1356)
                     /gene="uppS"
                     /locus_tag="VN444_05375"
     CDS             complement(580..1356)
                     /gene="uppS"
                     /locus_tag="VN444_05375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWR21275.1"
                     /translation="MFKHLLYRLYEGRLFQEVRGGELPRHIGLILDGNRRYAREYGYS
                     TILEGHRMGAEKLEEVLNWCEELEIRMVTVWIFSTENVSRSQEEVDDLMALIEKKMRD
                     IARDPKVHRKRMRIRAIGKMELLPPSTVKAIHEAEEATRDYDAFFLNVAVGYGGRQEI
                     ADAIAAMLRDKSAKGVPLEKIIDDISIDEIGKYLYTYDLPDPDLIIRTSGEVRLSGFL
                     LWQSAYSEYYFCDAYWPIFRKIDFFRAIRSFQQRERRFGV"
     gene            complement(1389..2477)
                     /locus_tag="VN444_05380"
     CDS             complement(1389..2477)
                     /locus_tag="VN444_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWR21276.1"
                     /translation="MASWLNPEEDALFFLEREMAGVFAITESRLLDGMVRFKGILLVD
                     PGKAVAELTKRLAPHGYYPLLRSQEDLTILRAMPSKRPFRSGPWLNILLLLATLVTTV
                     FVGATNRGAHPLANPWSVMQGLPFAITLLSILGVHEFGHYFTARWYGITVTLPYFIPA
                     PVGLGTFGAFIKMKSPVTDRRALFDVGIAGPLAGLCVALPAIVVGLHWSELILTGSEG
                     HVGIALGTPLMFSLLQWLTLGPIPQGGDVLLHPVAFAGWIGLLVTALNLLPIGQLDGG
                     HIAYALVGKHHRKVAIFALLVLVGMGTLYWPGWLFWASLSLILGLKHPPPLNDVTPLD
                     KRRRLVGFASLLLLLSVITPSPFNLSEL"
     gene            complement(2590..3522)
                     /locus_tag="VN444_05385"
     CDS             complement(2590..3522)
                     /locus_tag="VN444_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWR21277.1"
                     /translation="MMSAEHPQIRNFIVFSLLAHLLFVGLLSAMQLFTRLPADRPLQV
                     RIIDRSVRTRVSPPRDRRMLEKFAARAQRPSKKVAQQRTPNSSRFSRPQSAPLPTASL
                     TPPPASSAIAGSGAPSAHSAALTPWVPPPLRTAPGLAATPSPKVAHVEPLEGEGVDGS
                     SRSSDLRGQLAMLWKSLNAQQYPDQKADTGEEGDASSPAGPTVSLDSQDSRFAPYLLG
                     VKRRIESVWGYPQAARGLTGNLVVTFGITRDGHLSDLQLTQTSGIAPLDNEAIRAIRQ
                     ATPFSPFPERMHFERLNIRAAFYYHVSRGSLGDR"
     gene            complement(3593..4435)
                     /locus_tag="VN444_05390"
     CDS             complement(3593..4435)
                     /locus_tag="VN444_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR21278.1"
                     /translation="MAIRPGIRLDAVHFTYPSGFGLTGIDLCIGRGERVAILGPNGAG
                     KSTLLKLMLGLLHPGEGAISFEGSALGRMSRMELARTIAMVPQELLLPYALTAREVVL
                     LGRTPYLHRYRGPARDDLEAVQTAMVATDLLASADRPYNELSGGERQRVILAMALAQQ
                     PRLLLLDEPTRSLDLSHQLRILSLIRDLNVKHGLTVVSSMHDLNLSSLYFDRLVLLSS
                     GRIVADGPPDEVIQPDMIQEVFGVSVLVGQHPVRGIPWVTLLPDNLDLFSARKDASAE
                     TINP"
BASE COUNT          995 a         1356 c         1283 g          879 t
ORIGIN      
        1 ggtttgggga gccgctctgc cgcctccgaa gcgcattgcg cgtaccccat ggattgatga
       61 cgacgacgag cagaggtaga caaactaccg cctacctctg ctgttccggt tctctcacaa
      121 gctgcttgac ctgctgaggc gagacatagt atcgcccgcc gcaggtctgg ttggtcccgg
      181 ccgccagttc ttctcgaatg atctcactag tccccatggt cagtccatag accgtcccgc
      241 caagcgccac cgtcgcagca caggcgacaa gcttgagcgg aagcgtgatc acggtggcaa
      301 tcacgctgac cgcaccagcg gacacatccg ctcccaggct ggtctcctca gccctcgccc
      361 aggatgccac tgtcatctgc accaagaggc atagcgcgag gagcgctgtc agtcgtctgt
      421 taagcttcat gctatcaccc tcccgactcc aaggccatct gaggccgacc tttccaccgc
      481 cccggaacac gctttttctt cataccacac cttgcacttc gaaccttgaa cctcgcactc
      541 cgcaccagac atgggcgcag caagccgcgc ccctacattt cataccccaa accggcgttc
      601 gcgttgctga aagcttcgaa tggcccgaaa gaagtcgatc tttctgaaaa tcggccagta
      661 ggcgtcacaa aagtaatact cgctgtacgc gctctgccag agcaggaacc cgctcagtcg
      721 aacttcaccg ctggtccgga tgatgagatc gggatctggc aggtcgtatg tatagagata
      781 cttaccaatc tcgtcgatgg aaatatcatc gatgatcttc tccagtggga cacctttcgc
      841 tgatttgtcc ctgagcatgg ccgcgatggc atccgcgatc tcctgtctcc cgccatatcc
      901 cacggcgaca ttcaggaaaa aggcgtcgta gtcccgcgtc gcctcctctg cctcgtggat
      961 cgccttcacc gtggacgggg ggaggagctc catcttgccg atagccctga tccgcatccg
     1021 cttccggtgc accttgggat ccctggcaat gtcgcgcatc ttcttctcta tcagcgccat
     1081 gaggtcgtcc acctcttcct ggcttcggga tacgttttca gtggaaaaga tccacacggt
     1141 gaccatcctg atctcaagct cttcgcacca gttcaagacc tcttccagct tctccgctcc
     1201 catccggtgt ccttcaagga tggtggaata cccgtactct ctggcgtacc gccgattgcc
     1261 atcaaggatc agaccgatgt gccggggcag ctctccgcct cgaacttcct gaaacagccg
     1321 tccttcgtac agacggtaca gcagatgctt gaacaccccg tatcgttcct tcctcttcat
     1381 caaggggctt acaattccga taaattgaag ggggagggcg tgatcacgct cagcaaaagt
     1441 agcagcgatg cgaagcccac cagccttctg cgtttatcca gcggagtcac atcgtttagg
     1501 gggggaggat gtttcaggcc caggatcagg gagagggacg cccagaacag ccaccctggc
     1561 cagtacaggg tccccatgcc gaccagtacg agcagggcga agatcgccac cttccggtga
     1621 tgcttaccta ccagtgcata cgcgatgtgg ccaccatcca gttgaccgat cgggagcaga
     1681 ttcagcgccg tcacgagcag accgatccag ccggcgaaag ccaccgggtg caacagcacg
     1741 tcgcctcctt ggggtatcgg tcccagcgtc aaccactgca ggagcgaaaa catgagcggt
     1801 gtccctagag cgatgccaac atgtccctcc gagcctgtga gtatcagctc cgaccagtgt
     1861 agtcccacga cgatggctgg gagtgccacg caaagccctg caagcggtcc tgcgattccg
     1921 acgtcgaaga gcgccctgcg atcagtaacc ggcgacttca tcttgatgaa tgccccgaag
     1981 gttccgagtc caaccggggc agggataaaa taggggagcg tgactgtgat gccataccat
     2041 cgagcggtaa agtagtgtcc gaactcatgg acaccgagga ttgaaaggag cgtgattgcg
     2101 aaaggaagtc cttgcattac cgaccaagga ttcgccaggg gatgggcacc gcgattggtg
     2161 gcacccacga acacagtggt aacaagggta gccaacagga gcaggatgtt cagccatggg
     2221 cctgaacgaa acggacgttt tgagggcatc gcccggagga tcgtcaggtc ctcctggctt
     2281 ctgagcagcg ggtaatagcc gtggggggca agtcgcttcg tcagctcagc gacagccttg
     2341 ccgggatcga ccagtaaaat tcctttgaaa cggaccatcc cgtcaagcag gcgagactct
     2401 gtgatcgcaa agacgcctgc catctcccgt tcaagaaaaa agagggcatc ctcttcaggg
     2461 ttcagccaac ttgccatatc ttgcttatac cacggaccct gcgagtcgtc aaaaccttct
     2521 gtcgcctctc cgcgagagcc tgattcctca taacggcggg cacttcgacg cgtatcccga
     2581 tggtgaagct catcgatccc caaggcttcc acggcttaca tggtagtaaa aggccgctcg
     2641 gatattcagt cgttcgaagt gcatccgctc agggaagggg ctgaaggggg tagcttgccg
     2701 gattgcccga atcgcctcat tatccagcgg ggcgataccg gaagtctgcg tgagctgcaa
     2761 gtcgctcaag tggccatcgc gggtgatccc aaaggttacg acgagattcc cggtcaggcc
     2821 tcgcgccgcc tgagggtaac cccaaacact ctcgattcgt cttttcaccc caagcagata
     2881 gggcgcaaat cgagaatcct ggctgtccaa cgagacagtt ggcccagcag gagaactggc
     2941 atcgccttcc tctccggtat cagccttctg gtcaggatat tgctgagcat tgaggctttt
     3001 ccaaagcatg gccagttgac ccctgagatc ggatgagcga gacgacccat cgacaccctc
     3061 gccctcaagc ggttctacgt gagccacctt cgggctgggg gtggcggcta gcccaggcgc
     3121 tgtccgcagc gggggcggta cccaaggggt gagcgcagcg ctatgagcag aaggggcgcc
     3181 ggatcccgct atagccgacg acgccggtgg aggagtcaag cttgctgtag ggaggggagc
     3241 cgattgtgga cgcgagaaac gtgaactatt cggcgttctc tgctgggcta cctttttcga
     3301 aggccgttgt gcgcgcgcag cgaacttctc gagcatccta cggtcacgcg ggggtgagac
     3361 ccgcgttcga acagatcgat cgataatccg aacctggagt ggccgatcgg caggtagtcg
     3421 ggtaaacaac tgcatcgcgc tgagcaggcc gacaaagagc agatgggcta gcagcgaaaa
     3481 gacgataaag ttgcggatct gcggatgctc agcagacatc atccgcgacc cggcatcttg
     3541 aatcttgact cttaacacta gggattacaa tagtctgcag ccaccaaatt tgtcaagggt
     3601 tgatcgtctc tgcggaggcg tctttgcggg cggagaacag gtcgagattg tctggcaaga
     3661 gggtcaccca gggaataccg cgtacgggat gctgccctac aagtacggac acgccaaaga
     3721 cctcctggat catatccggc tggatgacct cgtccggggg gccatccgcc acgatgcgac
     3781 ccgatgatag aagaaccaga cggtcgaaat agagcgaaga caggttcaga tcgtgcatgg
     3841 atgagacaac cgtcagaccg tgcttcacgt tgaggtccct gatcagcgag aggatccgga
     3901 gctggtgact gagatcgagc gagcgagtcg gttcgtcaag aagcaacagg cgtggctgtt
     3961 gtgccagcgc catcgccagg atgacccgct ggcgttctcc cccggagagt tcgttgtacg
     4021 gacggtctgc ggaggccaga agatcggtgg cgaccatcgc tgtctgtact gcctccagat
     4081 cgtcgcgagc aggaccacgg tagcgatgca gatacggtgt ccgtcccagc agtaccacct
     4141 ccctggctgt gagggcatac ggcagcaata gctcctgggg aaccatggca atcgtccgcg
     4201 ccagctccat ccggctcatt ctgcccaagg cgctaccctc gaaagagatg gccccctccc
     4261 ctgggtgtaa cagacccagc atcaacttca ggagggttga ctttcccgct ccattcggac
     4321 ccaggattgc gactcgttca ccccgcccga tacacagatc aatgcctgta agcccgaagc
     4381 cactcggata ggtgaaatgc acggcatcga gacggatccc cggtcttatc gccatccgga
     4441 ctcccgcccg ttgcgtcgaa gcaagaaaag gaaaagggga ccgccaagca gagctgtgaa
     4501 aattcctaca ggg
//
LOCUS       DATMXI010000288         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_72213, whole genome shotgun sequence.
ACCESSION   DATMXI010000288 DATMXI010000000
VERSION     DATMXI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72213"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VN444_05395"
     CDS             <1..1172
                     /locus_tag="VN444_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR21279.1"
                     /translation="SVSSSADLSAIVDRKDRLIREFANYRIQQLRDPAFALYESPGRF
                     CSPREIDIAGARLSAHTFIIATGSSPSEVAIPGLSDVDFFTSDTILDLRTQPASLLVL
                     GAGPVALELGQFFARLGTKVTILQRGRTVLSQMDGDVGLALEAALKEDGIEVVTEAQV
                     LRVTCQEGRKSVWVQHHGQERVFAAEAILQALGRRPNIAGLQLDAAGVDVTGGRIVVD
                     AAMRTSQPHIFAVGDVNDLNPIVHLAIQQGEIAGYNATHAGKLARHIDHRLDAEVVFT
                     EPQVAVVGLNERMCRSKGIRYLTASYPFADHGKALCLGASHGFVKLLCTPPTGELLGA
                     QIVGPEAGELIHELIAVMYYHGTVADLLQMPHYHPTLAEIVTYPAESLMEQLSAG"
     gene            1278..1520
                     /locus_tag="VN444_05400"
     CDS             1278..1520
                     /locus_tag="VN444_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21280.1"
                     /translation="MARQSEPARINLHMVGMIARSVGIVAIALGFVIGIYGLEETGTW
                     WLQTALGLLVTGMLAQGCALYCSIKRMQQLHNRPKL"
     gene            1526..1942
                     /locus_tag="VN444_05405"
     CDS             1526..1942
                     /locus_tag="VN444_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21281.1"
                     /translation="MGRRLFTAGAILCAGAMLACGGPWRDRYFDKGVDRLTQEEISER
                     MGPPHTAKVPTLGGESVWTYRVPISDKDLASVTLSGLADPVQEVTALMGKVAPDSPKA
                     MLYCYRYTLTFNEQKVLKQWKREECTPGTHDTLNAN"
BASE COUNT          438 a          572 c          633 g          465 t
ORIGIN      
        1 cctcggtgtc gtccagtgca gatctgtcgg cgatcgtgga tcgaaaagat aggctgattc
       61 gcgaattcgc taattacagg atccaacaac ttcgcgatcc cgcctttgca ctttacgaat
      121 ctcctggtag gttttgctcg cctcgtgaaa ttgacatagc aggcgccagg ctctctgcgc
      181 acaccttcat tattgcaacc ggctccagcc cttccgaggt cgctatccct ggtctatcgg
      241 acgtagattt cttcacgagc gatacgattc tggacctgcg tacgcaacca gcttctctgt
      301 tggtattggg ggcagggcct gtagcactcg agctgggtca gttctttgcc aggctcggca
      361 cgaaggttac gattctccag aggggtcgca cggtcttgtc gcagatggac ggggatgtcg
      421 ggctggcgct tgaagcggcg ttgaaagagg acggcatcga agtcgtgacg gaggcgcagg
      481 tgctccgcgt cacctgccaa gagggtcgga aatcagtgtg ggtacaacat catggccagg
      541 aacgggtatt cgcggccgaa gcgattctgc aggcgttagg tcgtcgcccc aatattgccg
      601 ggctgcaact ggatgcggcg ggggtggatg tgacgggagg acggatcgtc gtcgacgcgg
      661 cgatgcgaac atcgcagcca catatctttg ccgtgggtga tgtgaacgat ttgaatccga
      721 tcgtgcatct cgccatccaa cagggtgaga ttgccgggta caatgcgacc cacgcgggaa
      781 agcttgcaag acacatcgat catcggcttg atgccgaggt ggtcttcaca gaaccacaag
      841 tagcagtcgt gggactgaat gaacggatgt gtcgctcgaa gggaatccgt tatcttaccg
      901 cttcttatcc ctttgccgat catgggaagg cgctgtgtct cggggcatcg catggcttcg
      961 tgaaactcct ttgtacgccg ccgacaggcg aactccttgg cgcgcagatt gtcgggcctg
     1021 aggcgggtga gctgatccat gaactgatcg cggtgatgta ttaccacggg accgttgccg
     1081 acctcttgca gatgccgcat taccacccca cgctggcaga aatcgtgacc tatccagcgg
     1141 aatcccttat ggaacagttg agcgcaggat gaaaagactc acgatgggtg agcgtcgatc
     1201 tgacgtgctt cttcgcacct tcagcatgga tctttcagag ttgaaccagc tgcgccatcc
     1261 agtgtagcgt actccctatg gcgagacaga gcgagccggc tcgaatcaat ctgcacatgg
     1321 tcgggatgat cgcacgatcc gtggggattg tggcgattgc actcggattc gtcatcggga
     1381 tctatggact ggaagagacg gggacctggt ggctgcagac agccttggga ttgttggtga
     1441 ctgggatgtt ggctcaaggg tgcgccctct actgctcgat caaacgcatg cagcaactac
     1501 acaacaggcc aaagttatga gaggaatggg tcggcgtctg ttcaccgccg gtgcgatcct
     1561 ctgcgcaggg gcgatgctgg cctgcggtgg cccctggcgc gaccggtatt tcgacaaggg
     1621 cgtcgatcga ttgacacaag aggagatttc tgaacgtatg gggccgcccc atacggccaa
     1681 ggttccgacg ctcggtggcg agtcggtctg gacctaccgc gttccgatca gcgacaaaga
     1741 cctggcctca gtgacccttt ccggcctggc tgatccggta caggaagtga cggcattgat
     1801 ggggaaggtt gctcctgata gccccaaggc catgttgtat tgctaccgct acactctgac
     1861 atttaacgaa cagaaggttc tgaaacaatg gaagcgtgaa gaatgcacgc cgggaaccca
     1921 cgatacgctc aatgcgaact agtattctga ttccctaatc catgcctgcc tggctaccgc
     1981 acatcgacag ctcgatcctt ctggacagcc tcaactcgtt cttgctgctc gtcacgctcc
     2041 tcatcacccg tgcgctgatt gtccgcgtga tcgccaagaa ctcggccctg tcgatggaag
     2101 ccaagcga
//
LOCUS       DATMXI010000289         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_133490, whole genome shotgun sequence.
ACCESSION   DATMXI010000289 DATMXI010000000
VERSION     DATMXI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133490"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VN444_05410"
     CDS             <1..605
                     /locus_tag="VN444_05410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWR21282.1"
                     /translation="RLEDVTGFTPMPQEYPLAASEAKFILIDLAEQFLGAYEYGRLAF
                     SAPIATGEKRNETPAGEFTITAAHRRHRSSLYTIEKTNIPYPMNYALLFHVSKEGVAF
                     WIHGRDIPGYPASHGCIGLYDEPMQKTHYGYPRDPILEDARTLYTWVLAPLPEDEKYR
                     ILDNGPRMHIVGQAPLPLRRSSQDGSRVRPVPRIAPEAMQ"
     gene            905..1225
                     /locus_tag="VN444_05415"
     CDS             905..1225
                     /locus_tag="VN444_05415"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HWR21283.1"
                     /translation="MLSKRIELKASRREVLQQMRQLGPWVEGTLVATARRCGKKNCAC
                     HHDGPKHPVLFLTWKEFGKTVSLYIPRAMEAEVRIWAENYKKLKELIRRASEAQKQLI
                     RLRD"
     gene            1486..2607
                     /locus_tag="VN444_05420"
                     /pseudo
     CDS             1486..2607
                     /locus_tag="VN444_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2869..3186
                     /locus_tag="VN444_05425"
     CDS             2869..3186
                     /locus_tag="VN444_05425"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HWR21284.1"
                     /translation="MDAATIRRLVAEGRYEFSKHAEREREADMIPTTELEEALKSSDI
                     IEDYPDDPRGPSCLVLGFAGQRPIHAVCSLRRDPEELFLITVYDPSKRPEHWMNHYSR
                     RRR"
     gene            3191..3439
                     /locus_tag="VN444_05430"
     CDS             3191..3439
                     /locus_tag="VN444_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HWR21285.1"
                     /translation="MARVYHCCYFCGGEVVEQRVTVDYRWGDAFLVVIRHVPAGVCEQ
                     CGEQYLKAEVVKEMEKVAHSQEEANEVLRIPVRELKVA"
     gene            complement(3417..>3851)
                     /locus_tag="VN444_05435"
     CDS             complement(3417..>3851)
                     /locus_tag="VN444_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HWR21286.1"
                     /translation="ALDVCPTPNAFHLLLDFPAEKAVESLRKEDYLMKAHLLEELVDA
                     MTVEHRIKPKGIRDAALLLYLNTHPTLKLDGAIALWRKGRISLAKAAEIAGLTVPEFK
                     DVLAAKEIVRDTEGKSAKAMDEKLHHLLPSFPEINPMPLSVP"
BASE COUNT          834 a         1091 c         1118 g          808 t
ORIGIN      
        1 ggcgcctcga ggatgtcacc ggcttcacac cgatgcctca ggagtacccc ttagcagcca
       61 gcgaggccaa gtttatcctg atcgatctcg cagagcagtt cctcggcgcc tacgagtatg
      121 gtcggctggc gttttcggcg ccgatcgcca caggcgagaa aagaaacgaa accccggcag
      181 gcgaatttac aattaccgct gcccaccggc gccaccgttc atccctctat accatcgaga
      241 agaccaacat cccgtatccg atgaactacg cccttctctt ccatgtcagt aaggaaggcg
      301 tcgcattctg gattcatggg cgtgatattc ccggatatcc agcctcgcac ggctgcatcg
      361 gactgtacga cgagccgatg cagaaaacgc actatggata tccccgcgac ccgatccttg
      421 aggatgccag gaccctctac acatgggtgc tggcgcctct gccggaggac gagaagtatc
      481 ggatcctgga caatgggcca cggatgcaca ttgtcggcca agcgccgctt ccgctgcgac
      541 gatcaagcca ggacggctca cgtgttcggc ccgtccctcg aatcgctcct gaagcgatgc
      601 aataagaggg ggctttcgcg tgccatcgct ccctccccca ggccgagcgt gagtatctgt
      661 gtaacccctt ggcgaatgtt catcgcgggg ttacctgaaa tacttccaat actccgtgcc
      721 attcacctag ccgtaatcct tcagtgaagc gcgggtcgtt ttatagcctt cccataaccc
      781 gaggtgcatt ctttctcgtc cgccgctgtt caaatcacat cttctgatcc gtgcacatca
      841 gatcactgac cttggcaact cattttcttc ttgcatgtcc gtaatgtata gatacaagac
      901 agtcatgctt tctaaacgta ttgaactcaa ggcctcgcgg cgagaggtgc tccaacaaat
      961 gaggcagctg ggtccgtggg ttgaaggcac cctggtcgcc accgctcggc ggtgcggcaa
     1021 gaaaaactgt gcctgtcacc acgatggtcc gaaacatccc gtgctctttt taacctggaa
     1081 agaattcggc aaaacggtgt cgctgtatat tcctcgcgct atggaggccg aagtgcgcat
     1141 ctgggccgag aactacaaaa agctcaaaga gttgatccgg cgcgcctcgg aagcacaaaa
     1201 acagctcatt cgcctgaggg attgatggcg ctcaacttct gttccgtctg ttttgacaag
     1261 caaatgacca tcgatcgttt ggaacaggag aatgcccggc tgcggcagaa gttgcgctcc
     1321 gaagagcgtc gaggcaagga aggatttttc ctctccgcga cgccctcgtc caagttgccg
     1381 ggaacgatca ataccccagc gccgaccttg cgcaagcccc ggggggcaag gcagggacat
     1441 caggggagcg gtcgcacgcg catcgaggaa accgctgcgg accggatgct tgacatcgcc
     1501 gcggatcccg aatgctgctg cccggtgtgc catggcatcc tggagcaaaa ggggtcccaa
     1561 tcgagacacg tcatcgagag ccgtccgatc agagccgagc gggaggtcta ccacttaccc
     1621 aagatgtatt gcccacgctg tcgcaaggcg taccacactc cgttgccggg cgtgctccca
     1681 cgcagtctgt acggtaacgc tctgattacg acggcggtga cgatgcacta tctgcacgga
     1741 attccgatgg gacgaatttg cgaacagatc ggcatcgggc cgggcagcct ggttgaagtg
     1801 ttccatcggc tctcgcgtct ttttgaacac gttccagaag gactgatcga gcaataccgc
     1861 acagcgccgg tcaagcacgc cgatgagacc ggctggcgca cggagggcaa gaacggctac
     1921 acgtggttgt tcgccacgcc gacgctgagc atttttcagt ttcgcaagac gcggtcggcc
     1981 gaggtgcccc gagcgatctt tggccaagag cccttgcctg gaactttagt ggttgatcgt
     2041 tacgccggct acaacaaggt cccgtgcgct attcagtact gctacgcaca tctgttgcgg
     2101 gaagttgaac gctgagaaca ggagtttccc gatgccgccg aagtcaaagc ctttgccagc
     2161 actctggcgc cgctgctggc gttggccatg ggcctgcgca atcaacacat ctcggatcaa
     2221 caattctctg ccaccagtgc cgaagtgaaa gcgcagatca tcgctgcggt cgatagtccc
     2281 gccgctcatc tgggtatccg tcgaatccaa aacatcttcc gcgaacatgc ttcgcgcctc
     2341 taccattggg cggcggaccg cgccattccg gctgagaaca accttgcgga gcgagacctc
     2401 aggcccaccg tgatcgcccg caaggtcagc ttcggctccc agtccgacgc cggggccaaa
     2461 accagaggaa tcctcatgtc cgtccttgtt actctgaaaa cacgcggctt cgatgtgacg
     2521 actcgcctca aagaagttct ggatgagatc gccaggaacc ccacccaaaa cgccttccca
     2581 ctcctgtttc caagagccgg tccgtgatct gtgccaggcc ggcgctcggt ttcactcgat
     2641 aacaacaata ttgctgcacg ttgagcacgg ctccgtcatc gaccaagggc gacatcccgc
     2701 gcttcactga aggattactc gaggatatct cccgttattg acagccgata gtgaccgggt
     2761 tacagtcgaa ggtgcttggg gcggtggaac tcggaaagag gggtcttgga gattgttcca
     2821 aggcattgga ggggggaaag agccatcgca tcaaaagagt ggtgaggaat ggatgcagca
     2881 actatacgcc ggttagttgc cgaaggacgg tatgaatttt caaaacacgc cgagagggaa
     2941 cgagaggccg atatgatccc gaccacggag ttggaagagg ccctgaagag cagcgacatc
     3001 atcgaggatt atccggatga cccgcgagga ccgagctgtc tggtcctcgg atttgcgggt
     3061 cagaggccta tccacgccgt gtgtagcctt cgacgagatc ctgaggagct gttcctcatt
     3121 acggtgtacg atccttccaa acggcccgag cactggatga accattatag ccggcgaagg
     3181 aggtgatgct atggccaggg tgtatcattg ctgctacttc tgtggcgggg aggtagtcga
     3241 gcaacgagtg accgtggatt atcggtgggg ggacgcgttc ttggtggtca ttcgccatgt
     3301 gccggctggg gtctgcgaac agtgtgggga gcagtatttg aaggctgagg tggtcaaaga
     3361 gatggaaaag gtggcccact ctcaggagga ggcaaacgag gtccttcgaa tccctgttag
     3421 ggaactgaaa gtggcatagg attgatctcc ggaaatgacg ggaggagatg gtggagtttc
     3481 tcatccatcg ctttggcgct cttgccctct gtgtcacgaa cgatttcttt tgcggccagc
     3541 acatccttga actccggcac ggtgagtccg gcaatctccg cggccttggc cagggagatc
     3601 cgtccttttc gccacagcgc aatcgctccg tcgagtttga gcgtggggtg tgtgttcaga
     3661 tacaggagaa gagccgcatc ccgaatcccc ttcggcttga tgcgatgctc gactgtcatg
     3721 gcgtctacga gttcttcgag cagatgtgcc ttcatcagat agtcctcctt tctcagtgac
     3781 tctactgctt tttctgcggg gaagtcaagg aggaggtgga aggcgttggg ggtggggcag
     3841 acgtcgaggg c
//
LOCUS       DATMXI010000290         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_39449, whole genome shotgun sequence.
ACCESSION   DATMXI010000290 DATMXI010000000
VERSION     DATMXI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39449"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VN444_05440"
     CDS             <1..248
                     /locus_tag="VN444_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21287.1"
                     /translation="THALAHTWIPDPRNIGNELLIVRLKLFVPSGATGYEIVVCRRPK
                     MGQPAHAPDTLRGSVRLLEESPVNRVTCSKMEVKDAH"
     gene            238..1005
                     /locus_tag="VN444_05445"
     CDS             238..1005
                     /locus_tag="VN444_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HWR21288.1"
                     /translation="MPIDIRKPLKRFLPYLLEARTNGLNEADTVLRLCRFFENVLGYD
                     GLQDISREAEMKRKYVDICVKVDGAVRLLVEAKAADQKLRDRHIEKAQSYASQNNFRW
                     VVLTNGMDWHLYHLTFDEGIEYERAFTVSLDNPDLFGDAAERLALLHKQSLKKGELER
                     FWEKATALGPGSIGKVLFTDRVLALIRREIRRDTGLLIDPEDLAKSLNEMLSAEAREQ
                     IGPVRVRKRRRRSKGAQTGRPSVEVGAESASNDPTTA"
     gene            1002..1154
                     /locus_tag="VN444_05450"
     CDS             1002..1154
                     /locus_tag="VN444_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21289.1"
                     /translation="MIARPGTGCTLRVGRTKGLQATAAMEAFGEIVAPRLGGRSLAAA
                     FGHETI"
     gene            1151..2068
                     /locus_tag="VN444_05455"
     CDS             1151..2068
                     /locus_tag="VN444_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21290.1"
                     /translation="MKVFNLFSKRQKRGRGEVPDVYQYKTIPDVLRVQVVHIMRDAFG
                     ELDRYNDGARNAYKFIHETLCREYGVFTLGGRHNSDFESVVDFLLQTQETEKAIDVIE
                     LSFRFLDRVVRENAYQYEGGKISPDDAIAELNYRFREHGVGYQYESGTIIRVDSQLIH
                     SEVVRPVLSMLSDPMYEGANAEFLSAHEHYRTRKYKECLNDCLKAFESCIKTICAKRG
                     WAYSASDTAKRLVEIVFERELIPTFMQSHFSALRSTLEAGVPTVRNRLSGHGQGSEEV
                     SVPESIAAYALHLTASNILLLARADDEMK"
     gene            2329..2547
                     /locus_tag="VN444_05460"
     CDS             2329..2547
                     /locus_tag="VN444_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HWR21291.1"
                     /translation="MRKHYDFEKMKGQRNPYVKQLKQPITIRLDKATITYFKALADEL
                     GIPYQNLINLYLRDCALHHKKLEFKRAS"
     gene            2628..2855
                     /locus_tag="VN444_05465"
     CDS             2628..2855
                     /locus_tag="VN444_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWR21292.1"
                     /translation="MRWQDRITITPGVRSGKPCIRGTRITVYDVLEYLAGGMTDAQIL
                     ADFPDLTAEDIHACLAFAAARERRLASPPAA"
     gene            2852..3199
                     /locus_tag="VN444_05470"
     CDS             2852..3199
                     /locus_tag="VN444_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWR21293.1"
                     /translation="MKLLFDENLSPGLVQSLEREHPGSAHVRTLGLLGATDWAIWERA
                     RLDVYTIVSKDNDFRQLSFLHGTPPKVIWLSVGNAGTGAILHFLRSQRAVIQAFETDT
                     EASLLVLTLTEEV"
BASE COUNT          841 a          923 c          958 g          748 t
ORIGIN      
        1 cgacgcacgc attggcgcac acatggattc ctgatccgag gaatatcggc aacgagttgc
       61 tcattgtgcg tttaaagctg tttgtgccaa gcggcgcaac gggctatgag attgtggtct
      121 gtcgtcggcc gaaaatgggc cagccagcac acgcaccgga cacgcttcgt ggctccgttc
      181 ggctgcttga ggagtcacct gtcaatcgag ttacttgttc aaaaatggag gtaaaagatg
      241 cccattgata tccgcaagcc cctgaagcgc ttcctgccat atcttcttga ggcacgtacc
      301 aacggtttga acgaggccga tacagtcctc cgactctgtc gtttttttga gaacgttctc
      361 ggctacgacg gccttcaaga catcagtcga gaggccgaga tgaagcgcaa gtacgttgac
      421 atctgcgtca aggttgatgg cgcagtacgg cttcttgtcg aagcgaaggc cgccgaccaa
      481 aagctccgtg atcgccacat tgaaaaggca caatcctacg cttcgcaaaa caacttcagg
      541 tgggtcgttc ttacgaacgg catggattgg cacttgtacc acctgacctt tgacgaaggc
      601 atcgagtatg aacgtgcatt tactgtctca ctggacaacc cagatctctt cggtgatgct
      661 gcagagcgcc tcgcccttct acacaagcag tccctcaaaa agggagaact agagcgcttc
      721 tgggagaaag ccactgctct gggtccgggg tcgatcggca aggtactgtt cactgacaga
      781 gtcctcgcac tcatcagacg tgagattcga cgcgataccg gcttgcttat cgatcccgag
      841 gatctcgcca agtccctcaa cgaaatgctt tccgcggagg cacgggagca gatcgggccc
      901 gtgcgagtcc ggaagcgtcg gcgccgatcg aagggtgcgc agaccggtcg accatccgta
      961 gaggttggcg ccgagtccgc cagcaatgat cctacaacgg catgatagct cgtcctggaa
     1021 cagggtgtac cctgcgcgtg ggccgaacaa aagggctgca ggcgacggcc gccatggagg
     1081 cattcggtga aatcgttgca ccgcgtcttg gcggccgctc cttagccgcg gccttcggtc
     1141 atgaaacgat atgaaagtct ttaacctttt ctcgaaacgg cagaagcgtg gtcggggcga
     1201 agttcctgac gtataccagt acaagaccat tcctgatgta ttacgggttc aggttgtcca
     1261 catcatgcgg gatgccttcg gggagctcga tcgctacaac gacggtgcca gaaatgcata
     1321 caagttcatc cacgagaccc tgtgccgcga atacggtgtc ttcacactcg gcggacgcca
     1381 taattccgac ttcgagtcag tcgtcgactt cctccttcag acacaagaga cagagaaggc
     1441 aattgatgtc attgaactat cattccgctt tctagatcgc gtcgtccgtg aaaatgcata
     1501 tcaatacgag ggagggaaga tctcacccga cgatgcgatt gctgagctga attacaggtt
     1561 ccgcgaacac ggcgtaggat atcagtatga atccggcact ataatccgag ttgattctca
     1621 gttgatccac tctgaagtag tgcggcctgt actgagtatg ctctccgacc caatgtacga
     1681 aggcgcaaat gcggaattcc tgagcgcaca cgaacactat cgaacgagaa agtacaaaga
     1741 atgcctgaac gactgtctca aggcatttga gagttgtatc aaaacgattt gcgcaaagag
     1801 ggggtgggcc tacagtgcca gcgataccgc caagcggctg gttgagattg tattcgagcg
     1861 cgagttgatc ccaactttca tgcaatcaca cttctccgca ctgaggagca cgttggaggc
     1921 tggtgttccc actgttcgca atcgcctatc aggacacgga caagggagtg aagaggtgtc
     1981 cgttccggaa tctatagctg catacgctct tcacttgacg gcatccaaca ttctcttgtt
     2041 ggcgagagct gatgatgaga tgaaatagga accgaacaat gccttctagg tgatacctaa
     2101 gggcacgcct gaaggcttac gttcggccac aataaggcct tgatcctcct acaagacata
     2161 cgtacagcgt acatacagtg tacatgtgga tgacattcag tccgagtggg atgaagagaa
     2221 gagttgtgtg ccactgcttt agggtgggcg atgccgtcat ccggatcatt tccgcacgga
     2281 aagcaaaccg aagggaggag gcggtctatt ggaactggag atgacatcat gagaaagcat
     2341 tacgatttcg agaagatgaa gggtcagcgg aacccctacg tcaagcagct taagcagccc
     2401 attacgatcc gccttgacaa ggcgacgatc acctacttca aggcactcgc cgatgagctt
     2461 gggataccct accagaacct tattaacctc tacttgaggg attgtgcttt gcaccacaag
     2521 aagctagagt tcaaacgggc atcatgaagg ggccgaccat ggcagtcgag cgtaccgctg
     2581 gctcgcatgc agtcgccagc cagccaagga gggataggct tatgaccatg cgctggcagg
     2641 atcgcattac catcactccc ggggtgcgga gcggaaagcc ctgcatcagg ggaacccgta
     2701 ttaccgtgta tgacgtcctc gaataccttg ctggggggat gaccgatgcc cagattctgg
     2761 ccgattttcc cgacctcaca gcagaagata ttcacgcgtg tctggccttc gcggctgccc
     2821 gcgaacgccg gctcgcgagc cctccagcgg cgtgaagtta ctcttcgacg agaacctgag
     2881 tccggggctc gtccaatccc tcgaacggga gcatccaggg agtgctcatg tccgcacgct
     2941 gggcttactg ggggcgacag actgggcaat ctgggagcgg gcgcggctcg atgtgtacac
     3001 gattgtctcc aaagacaacg actttcggca gctcagtttc ctccacggca ctccgcccaa
     3061 ggtcatctgg ttatcggtgg gcaatgctgg gacgggggcc atcctgcact tcttacgaag
     3121 tcagcgcgca gtgatccagg cttttgagac tgataccgag gcttccctcc tcgtccttac
     3181 gctcactgaa gaggtatgat cggtcgggga gataggtaac aaaaaagtcc ggggacatgg
     3241 gtgacaccct gagacatttg acgttaccat aatcattctc gacgcgcatg tatcgttcat
     3301 gcaaccaacc gagtatggaa atgagcgctc tcacgaaaga ccttcaaagt cattgggcca
     3361 atatccgccc catcctaacg attcgcaatg agcgcgaata tgactcagcg attaagcgat
     3421 taaataagtt gttggatgaa atcggtgata atgagcgcca tcccctgtat
//
LOCUS       DATMXI010000291         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_135332, whole genome shotgun sequence.
ACCESSION   DATMXI010000291 DATMXI010000000
VERSION     DATMXI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135332"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VN444_05475"
     CDS             <1..446
                     /locus_tag="VN444_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HWR21294.1"
                     /translation="LGPLPLESQVFQNGAASQTWVAVAADAPAERIETLLRRGLTVLE
                     AGGSHGRVSLEHLLKRLGEREVASVMIEGGEGIFTSAVEEGIVDKFLLFVAPLLVGGK
                     TAPSLFGGAGVEEIGQALRLVRLHIEQLGEDLLIKGYRSRHEGTC"
     gene            456..1106
                     /locus_tag="VN444_05480"
     CDS             456..1106
                     /locus_tag="VN444_05480"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWR21295.1"
                     /translation="MFTGLIEEVGHVQRVRREGVIAKLAITGQQSASSLSIGDSIAVD
                     GSCLTVTMTAHALFEADLSGETLTRTTLGELQVGDPVNLERPCRPTDRLGGHFVTGHI
                     DGVGTIREIRETGGMWWFSIAYPEALRPLLVEKGSVAMDGISVTVAALNDRSFGAAII
                     PHTYHNTTLRSKQVGRRVNLEADLLGKYVVRYLEGLESNTSKAALTESRLRDLGFA"
     gene            1127..2341
                     /locus_tag="VN444_05485"
     CDS             1127..2341
                     /locus_tag="VN444_05485"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWR21296.1"
                     /translation="MEKTIHFHTIEEAIRDLHDGKMVIVIDDDGRENEGDITMAAEKV
                     TPEAINFMATQGRGLICMPVIGERLDELRIPQMVTDNTSPHETAFSVSVDAKHKVTTG
                     ISAHDRAITIHTIIDPKATADDLTSPGHTFPLRGREGGVLTRAGHSEAAIDLARLAGL
                     YPAGVICEVMHDDGTMARTPELHRFARTHGLKLITVKSLIEFRLKRERFVSRIATTTL
                     PTPYGRFTAILYHSQIEKKDHLALIMGEINAVEETLVRVHSECLTGDVFGSLRCDCGP
                     QMEKSLELIASEGKGVFLYIRQEGRGIGLANKLRAYELQDAGLDTVEANLQLGFKPDL
                     REYGIGAQILSDLGLCNIRLLTNNPRKIVGLEAYGLHVVQRIPIEVHPTAENRAYLQA
                     KQEKLGHLLETL"
     gene            2434..2898
                     /gene="ribE"
                     /locus_tag="VN444_05490"
     CDS             2434..2898
                     /gene="ribE"
                     /locus_tag="VN444_05490"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWR21297.1"
                     /translation="MKTTEGIYLAKGFRFALVVTRMNELITKRLLEGALDCLFRHGAE
                     DGDLHLVKVPGAFELPYAAKRLAASNRYDAIIALGAVIRGATAHFDYVAGEAAKGIAA
                     TSLDTGVPVIFGVITANTLEEAIERAGTKGGNKGFDAALSAIEMASLFAQLT"
BASE COUNT          663 a          877 c          955 g          605 t
ORIGIN      
        1 ggctcggtcc gctcccactg gagagccagg tctttcagaa tggagccgcc tcacaaacct
       61 gggttgccgt ggccgccgat gcgcccgctg agcggatcga gactctgctg cgccgtgggc
      121 tgacggtgct cgaagctggc ggctcccatg gacgcgtcag ccttgagcac ctcctgaagc
      181 gcctcggcga gcgcgaggtc gcgagcgtga tgatcgaggg cggagagggc atcttcacct
      241 ccgccgttga agaggggatc gtcgacaagt ttctactatt cgtggccccc ctgctggtgg
      301 ggggaaagac cgctccaagt ctttttgggg gagcaggtgt tgaagagatc gggcaggccc
      361 tccggctggt ccgcttgcac atcgaacaac tgggggagga cctactgatt aaggggtacc
      421 gctcgaggca tgaagggacc tgctaggcac atcggatgtt taccgggcta atcgaagagg
      481 tgggtcacgt tcagcgggta cgccgggaag gcgttatcgc aaagcttgcc atcacagggc
      541 aacagagcgc ctcttccctc agcatcgggg acagcatcgc ggtcgatggg agctgcctca
      601 cggtcacgat gacggcacat gccctgttcg aggccgatct gtctggggag accctcacac
      661 gtacaaccct gggagagctc caggtcggcg atcccgtaaa tctggaacgg ccctgtcggc
      721 cgacggatcg actcgggggc cacttcgtga ccggccacat cgacggggtc ggaaccatcc
      781 gcgagattcg ggagaccggg gggatgtggt ggttctcgat cgcctaccct gaagcactgc
      841 ggcctctgct tgtggaaaag ggatccgtgg cgatggatgg gattagcgtg acggtggctg
      901 ctctgaatga ccgctctttt ggggcggcca tcattccgca cacctaccac aataccacac
      961 tgaggagcaa gcaggtcgga cggcgcgtca acctggaagc ggacctgctt ggcaaatacg
     1021 tcgtccgata tcttgaggga ctcgaatcca acacgagtaa ggcagcgctc accgaatcgc
     1081 gactccggga tctggggttc gcttgagtcg agtgtacgga gtagccatgg agaaaacgat
     1141 ccattttcat actatcgaag aagcgatccg ggacctgcac gacggcaaga tggtgatcgt
     1201 catcgacgac gacggtcggg agaacgaggg cgacatcacg atggccgccg agaaggtaac
     1261 cccggaggct attaacttta tggccacgca ggggcggggg ttaatctgta tgccggtcat
     1321 cggcgaacgg ttggatgaac tccgcatccc ccagatggtc acggacaaca cctcccccca
     1381 cgaaaccgcc ttctctgttt ccgtcgatgc caagcacaag gtgacaaccg gaatctcggc
     1441 ccatgaccgg gcgatcacca tccacactat catcgaccct aaggcaacag ccgacgattt
     1501 aaccagtcca ggacatacct ttccgctccg cggacgggag ggaggggtgc tcactcgggc
     1561 aggacattcc gaagccgcta ttgacctcgc cagattggcc ggcctgtacc ctgccggggt
     1621 gatctgcgag gttatgcatg atgacggtac gatggcccgg acgccagagc tacatcgctt
     1681 tgctcgtacg cacggtctaa agctaattac ggtcaaaagt ctgatcgaat ttcgattgaa
     1741 gcgagaacga ttcgtcagtc gcattgccac gaccaccttg ccgactccat acggccgatt
     1801 caccgcgatc ctgtatcaca gccagatcga gaagaaagac catctggccc tgatcatggg
     1861 agagattaac gccgtcgaag agacgctggt gcgggtccac tccgagtgtc ttaccggcga
     1921 tgtcttcggt tctcttcggt gcgattgcgg accgcaaatg gagaagtcgc tcgagttgat
     1981 tgctagcgag ggaaaggggg tctttctgta tattcgacag gaaggcagag gcatcggcct
     2041 ggcgaacaag ctgcgggcct atgagctgca ggacgctggc ctggacacgg tggaggccaa
     2101 tctgcagttg gggttcaagc ctgatctcag agagtatggg atcggcgccc agatcctgtc
     2161 tgaccttgga ttgtgcaata ttcggctgct gaccaacaat ccacgcaaga tcgtcgggct
     2221 cgaggcgtac ggtctccatg tagtgcagcg gattcccatc gaggttcacc caacggccga
     2281 gaaccgtgcg tatctgcaag ccaaacagga gaaactgggc catctgctcg aaacgttgta
     2341 gggaaaatca gcaatcagct atcagcgttc agccgtcagc tacgtgctga ccgcagagtg
     2401 ctgaacgatt cctttctgcc acggaggtgt gcgttgaaaa cgaccgaagg aatatacctt
     2461 gccaaagggt ttcgattcgc cctggtggta actcgcatga atgagttgat caccaaacgg
     2521 ctgctggaag gggcgcttga ttgtctgttc cgtcacggtg cggaggatgg cgacctacat
     2581 ctagtgaagg tccccggcgc ttttgagctg ccgtacgcgg caaagcgctt ggccgcctcg
     2641 aaccgctacg acgccattat cgcgcttggt gcggtcatcc gaggcgctac cgcccacttc
     2701 gattacgtcg cgggagaggc ggcaaagggg atcgccgcga ccagtctcga cactggtgtg
     2761 cccgttatct ttggcgtcat caccgcaaac acgcttgagg aggcgatcga acgggccggg
     2821 acgaagggcg gcaacaaggg attcgacgct gcgctttcgg ctattgaaat ggccagtctc
     2881 ttcgctcaac tcacatagtg tacacgcgag agggttctac ccctcacacg ggcaatggat
     2941 aaacgccacc gagccaggga gcttgcgctc tcactcctgt atcaacttga atttcacccg
     3001 ctagaggctt ttgaggagca gtatcaggcc ttctgggcag agcaccccat caaatcagag
     3061 acccagtcgt tggctgatga gctcgtccgg ggaaccctcc
//
LOCUS       DATMXI010000292         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_2076, whole genome shotgun sequence.
ACCESSION   DATMXI010000292 DATMXI010000000
VERSION     DATMXI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /gene="ilvB"
                     /locus_tag="VN444_05495"
     CDS             complement(<1..1622)
                     /gene="ilvB"
                     /locus_tag="VN444_05495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWR21298.1"
                     /translation="MKLTGSEILLRSLVEEGVDTVFGMPGGVILHTYDVMTDSAAAPK
                     IRHILCRHEQGATHAAEGYYKASGKVGTVMVTSGPGACNTVTGVTDALLDSMAIVVFT
                     GQVPTTAMGCDAFQEADVVGTTRTCTKHNFLALKTEDIPRIVKEAYYIARSGRPGPVL
                     VDLPKNVIMGRAEYRGHPQELSIRGYKPTTQGHAGQIKRAVELMRTAKRPLIYGGGGI
                     IHSEAYEELRELVAMTRIPVGLTLMGLGAFDTSDPLSLGMVGMHGTYWSNMAMIHCDL
                     MIAIGSRFSDRVTGKLSEFGKQCKIIHIDIDPTSIRKTVHVDVPIVGDVRQVLGELNK
                     EVCQIERNWAGHFEEWYAQIAEWKAKHPLRYAPMQDIIKPQYAIDTVFEVSEEMNPII
                     ATGVGQHQMWAAQRYRGHRPRRWLSSGGLGTMGYGFPAAMGAQAAFPGDLVICIDGDG
                     SFQMTNQDLITCVENNLPVKVVIINNGYLGMVRQWQDLFYNRRYSQVDLTVQPDFVKL
                     AEAYGAVGLRARRPDEVKPVLEQAFATPGPVIV"
     gene            complement(1769..>3398)
                     /gene="murJ"
                     /locus_tag="VN444_05500"
     CDS             complement(1769..>3398)
                     /gene="murJ"
                     /locus_tag="VN444_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWR21299.1"
                     /translation="GETVLLIKMERHSLVKDFLMEQERPGRIARAAGVVSGATLLSRI
                     LGFVRDLIIARAFGAGTATDAFFAAFRLPNMLRELLGEGALSAAFIPVFTEAFTKRGR
                     ESAWRLAWTILTLLTVLLVLVSIAGILLAPWLIRLIAPGFQAIPSKLHLAIYLTRLMF
                     PYILFIGVAALFMAILNSQGHFATPALSPSILNIAMIGCAIFLTPYVDPPIMALAIGV
                     LIGGVGQLLIQIPVVWKRGMGAHRGFDLSDPAVGRITRLMTPGVAGLAITQVNVFIGT
                     LLASLMGEGGISILYYAFRLIQLPIGVFGVAIATAAFPAMARQAANRSLGEVGGTVAY
                     AIRLVLFVTLPAMVGLMVFRIPIVQLLFERGAFDRTVTLATAEVVLFYAFGLGAYVSN
                     RILVPAFYSLQDTATPVKIGMVAVLVNIAASLLLMRPLGLAGLALATTLSSFVNLGLL
                     LIALRRRLGQLPGLSFRSLAPIGGAAVAMALIAVSLVHFRDPVTVEPVLRRAGLLTFE
                     LVTSFTVFLAAVACMGSEELKGLLQWFIGWRRRV"
BASE COUNT          775 a         1042 c          937 g          644 t
ORIGIN      
        1 acaatgaccg gtcctggtgt ggcgaaggcc tgctccaaga cgggcttgac ctcgtccggc
       61 cgccgggcgc gaaggccaac cgccccatac gcttctgcga gcttcacaaa gtccggctga
      121 accgtcaggt ccacctggga atatcgccgg ttgtaaaaga ggtcctgcca ctgccgaacc
      181 atcccgagat aaccgttgtt gataatcacc accttgactg gcaggttatt ctcaacgcag
      241 gtaatcagat cctgattggt catctggaag ctcccgtcgc cgtcgataca aataaccaga
      301 tctccgggga aggccgcctg ggcgcccatg gccgctggga atccatagcc catcgtccca
      361 agaccgcctg aggacagcca tcggcgcggc ctgtggcctc gatagcgctg tgccgcccac
      421 atctggtgct ggcccacgcc ggtcgcgata atcgggttca tctcctcact gacctcgaag
      481 accgtgtcaa tggcatactg cggcttgatt atgtcctgca tcggggcata ccgtagcggg
      541 tgtttggcct tccattccgc gatctgcgca taccactcct caaagtggcc ggcccaattc
      601 cgctcgatct ggcaaacttc tttgttgagt tcaccaagca cttgcctgac atcgcccaca
      661 atcgggacat cgacatgcac agtttttcta attgaggtag gatcgatgtc gatatggata
      721 atcttgcact gtttgccgaa ttcggacagt ttgccagtca cccgatcgct aaatcgagac
      781 ccgatggcga tcatcagatc gcaatgtatc atcgccatat ttgaccagta ggtcccgtgc
      841 attcccacca taccgagcga gagcggatcg gatgtatcga atgcgcccaa gcccatcagt
      901 gtcagcccaa ctgggatgcg agtcatggcg accagctcac ggagctcttc gtacgcctcc
      961 gagtgaatga tccccccgcc accgtatatc agcggccgct tggcggttcg catcaactca
     1021 accgcccgct taatctggcc ggcatggcct tgcgtcgttg gcttgtaccc gcggatgctc
     1081 aactcttgcg gatggccgcg atactctgcg cgtcccataa tcacgttctt tggaaggtcc
     1141 accagcaccg gccctggtcg gccagagcgg gcgatgtagt acgcttcctt gacaatccgg
     1201 gggatatcct cggtcttgag ggcaaggaag ttatgcttgg tacaggtgcg cgtggtgccg
     1261 acgacatcgg cctcctgaaa ggcgtcgcag cccatggccg tcgtaggcac ctgaccggta
     1321 aagaccacaa tcgccatcga atccagcaag gcatcggtta cacctgtaac ggtattgcag
     1381 gcccccggcc cggaggtcac catgacagtc cccaccttac cgctggcctt atagtaacct
     1441 tccgcggcgt gcgttgcgcc ctgctcatgc cggcagagga tatgccggat ctttggggct
     1501 gcggcactat cggtcatcac gtcataggta tgcaggatca cgccgcccgg catgccgaag
     1561 acggtatcaa cgccctcctc gaccagactc ctcagtaata tctccgatcc cgtcaatttc
     1621 attcccgtat ctcctcgtac ccctgacccc ctaaacttac agctttttcg agacgccgaa
     1681 ttggctcagc atcgattggt aacttgccaa tttatctagc acgtccccct cttagcgtca
     1741 atgaaaatct tcccctacgg ggtatccatc agacccgtcg cctccagcca atgaaccact
     1801 gcagtaagcc tttcagctcc tcagaaccca tacaggctac cgcagcgagg aatacggtaa
     1861 aacttgtcac aagctcgaac gtgagcagcc cagcgcgacg aagcaccggt tcaaccgtca
     1921 ccggatcacg gaagtggaca agcgatacgg caatcagagc cattgcgacg gcagccccgc
     1981 ctatcggcgc gagcgagcgg aagcttaacc ctgggagctg ccccagacgc cgcctcaggg
     2041 caatcagcag cagacccagg ttcacgaagg atgagagggt tgtcgccagg gccaagcccg
     2101 caagtcccag tgggcgcatc agcaagagag aagcggcaat gttgaccagg acggccacca
     2161 taccgatctt gaccggcgtc gcagtatcct gaagcgagta gaaagctgga acaaggatcc
     2221 ggttcgatac ataggcccca aggccgaagg cgtagaacaa cacgacctcg gcggtggcga
     2281 gggtcacggt ccgatcaaat gcgccccgct caaagaggag ttggacaatc gggatcctga
     2341 acaccatcag acctaccata gctggtagtg tgacgaagag aacaagacgg atcgcgtagg
     2401 ccacagtgcc cccaacctct ccaagggaac ggtttgccgc ctgtctggcc atggcgggga
     2461 acgctgccgt ggcaattgcc acaccaaaga caccgatcgg aagctggatc agccgaaagg
     2521 cgtagtacag gatcgagatc cccccctccc ccatcagcga agcgagtaag gtcccgataa
     2581 aaacattgac ctgcgtgatg gccagtcctg cgacccctgg cgtcatgaga cgtgtaatac
     2641 gcccgacggc aggatcgctg agatcgaatc cccggtgggc gcccatccca cgtttccaca
     2701 ccactggaat ttggatcagg agctgaccta cgcctccgat caggacgccg attgccagtg
     2761 ccatgatcgg cggatctaca tagggagtca gaaaaatggc gcacccgatc atggcgatgt
     2821 tcagtatgct gggggacagc gccggcgtgg cgaagtgacc ctgcgaattg agaatcgcca
     2881 tgaagagcgc cgccaccccg ataaatagga tatacgggaa catcaatcgg gtgagataga
     2941 tggcaagatg aagtttcgac gggatggctt ggaatccggg ggcgatgagc cgaatcaacc
     3001 acggcgcaag caaaataccg gcgatggaga ccagtacgag cagtactgtc aacagggtca
     3061 agatagtcca tgccagccgc caggcactct cgcgtccacg cttggtgaac gcttcagtga
     3121 aaaccgggat gaacgctgcc gagagggccc cctccccaag tagctcgcgt aacatgtttg
     3181 ggaggcggaa ggcagcaaag aaggcgtcgg ttgcggttcc ggcgccgaac gccctggcaa
     3241 tgatcagatc gcggacaaag ccaaggatac ggcttaacag tgtggccccg cttaccacac
     3301 cggcagctcg cgcgatcctc ccgggacgtt cctgctccat cagaaagtcc ttgacaagtg
     3361 agtgccgctc cattttaatg aggagcacgg tttctcct
//
LOCUS       DATMXI010000293         7542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_176585, whole genome shotgun sequence.
ACCESSION   DATMXI010000293 DATMXI010000000
VERSION     DATMXI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176585"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VN444_05505"
     CDS             complement(<1..257)
                     /locus_tag="VN444_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWR21300.1"
                     /translation="MNEETIQHILSRYPDRRSALLPLMHLWQEEAGYLTEGAMRELAA
                     RLDLPPTQVAEVAMFYDMFRLKPGGRYEIWVCHNLSCALLGA"
     gene            complement(308..1462)
                     /gene="nuoD"
                     /locus_tag="VN444_05510"
     CDS             complement(308..1462)
                     /gene="nuoD"
                     /locus_tag="VN444_05510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWR21301.1"
                     /translation="MTINMGPQHPSTHGVLRLVLELDGEIVVRCTPHIGYLHTGMEKI
                     AESKRYQQVIPITDRMDYLAPLSNNLAYVLAVEKLLDIEVPQRAKVIRVMLTELTRIG
                     SHLVWLATHAIDIGAMSVFLYAFREREAILDMSEQVSGARMMSSYFRIGGLFADLPEG
                     FEGVVHAFVESFPDRLAEYEDLLTRNPIWIERTKGVGVIKPEDAIDLGLSGPSLRACG
                     IPWDIRKANPYSGYEQFGFEVSRGIHGDVYDRYLCRIFEMQQSVAIVRQTLECLPEGP
                     IAVANPKLTPPPKPMIKQSMEALIHHFLLWSAGFAVPAGEVYQSIESPRGEYGVYLVS
                     DGSNKPYRLHFRTPSFVNLESLSRMVEGRLVADLVAIIGSIDIVLGEVDR"
     gene            complement(1473..1979)
                     /locus_tag="VN444_05515"
     CDS             complement(1473..1979)
                     /locus_tag="VN444_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWR21302.1"
                     /translation="MDNVKGVEENRTVSQLRERFPEATLPARSFRNETTILVRPGDII
                     RICRHLKEDPGLLYDFLSDLTAVDRLGDHPRFEVVYHLYSLQYKWRIRLKVPVEEGEA
                     VPSVTAVWSAANWHEREVFDMFGIGFDGHPDLRRILMPEEWEGFPLRKDYPVQSSPKW
                     WAEGAAGD"
     gene            complement(1967..2326)
                     /gene="ndhC"
                     /locus_tag="VN444_05520"
     CDS             complement(1967..2326)
                     /gene="ndhC"
                     /locus_tag="VN444_05520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWR21303.1"
                     /translation="MLFSYLPIFILILLVAGFALATLFVSHALGPRRPTQAKLAPYEC
                     GIDPVGSARERFSVKFYLVAMLFIIFDIEVVFLYPWAVILNSLRLFGLVEMIVFLGIL
                     LIGLLYVWRRGGLEWTT"
     gene            complement(2375..2593)
                     /gene="atpE"
                     /locus_tag="VN444_05525"
     CDS             complement(2375..2593)
                     /gene="atpE"
                     /locus_tag="VN444_05525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWR21304.1"
                     /translation="MAIASGAAAIGQSRAIVSALEAIGRQPAAAPRIQVAMIIGLALI
                     ESLAIYVLLVALIIFFANPFIKYVVPGA"
     gene            complement(2769..3488)
                     /gene="atpB"
                     /locus_tag="VN444_05530"
     CDS             complement(2769..3488)
                     /gene="atpB"
                     /locus_tag="VN444_05530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWR21305.1"
                     /translation="MEHHPTLIQIPNFLGALGVPGAWVPEHVAMAWVVMAILVGVSYL
                     ATRRLDAVPGPMQNFMEVVVEMFVDLLTQMIGPKGKRYLPLIGTAGLFILVGNLLGTV
                     PGFKPPTANLNTTAALAITVFLSYNYFGIREQGIAAYLRHFCGPILWLAPIMFPIEFI
                     GHLARPISLSIRLFGNIFGEESVIAILLSLIWLGIPYVIYLGIMMPLSLFTSFVQSFI
                     FVMLSMVYIAGAVQSEHEEHH"
     gene            complement(3537..3752)
                     /locus_tag="VN444_05535"
     CDS             complement(3537..3752)
                     /locus_tag="VN444_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWR21306.1"
                     /translation="MKDNQARLWRQLAGLSSLGITFAASIAIGAAIGIALDRWLGTSP
                     WLTILFFIFGVAAGFTNLLKDLKRWGS"
     gene            complement(3935..5386)
                     /locus_tag="VN444_05540"
     CDS             complement(3935..5386)
                     /locus_tag="VN444_05540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWR21307.1"
                     /translation="MTDVFNMVDISTASPEVVMGLVAMVILMLDFIAPKGGRDWLGYL
                     SILGVLATFTTLMGQRGATQLAFSGQYLSDPFAFFFKIVFLVSAALILLMSIGHLKSE
                     GIDKGEFYGLILFATLGMMLMVSAVDLLILYIGLETMSISIYILAGFLKRERRSGEAA
                     LKYLLMGGFSSAIMLYGIVMLYGLTGTIGLREIASTLSADTVSNPALILGMVMLVAGF
                     GFKIAAVPFHMYIPDVYEGAPTPVAALLAAASEAAGLAILLRVFLVAIPELQDRWTLL
                     FYALSLLTMTVGNVVAIAQSNIKRMLAYSSIAHIGYLLIGVVAGRELGISAILLYTLI
                     YALMTLGVFAMVILLCVGKVKGERIDDFTGLAQRSPMAAAAMLIFLLSLAGVPPTAGF
                     VGKLYLFGAAIEQGYIWLAVIAVINSAISVFYYMKVVVAMYMRDLPPQGLTLSASRPL
                     RVALFVTLAGTIAIGIYPGPFLELARASVAGLW"
     gene            complement(5379..7046)
                     /locus_tag="VN444_05545"
     CDS             complement(5379..7046)
                     /locus_tag="VN444_05545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWR21308.1"
                     /translation="MNVLQFPILSLMTYLPLVGVFFISLLPKECKGGVRWTALAFTIA
                     NLLASLWIPANFDSTTAEMQFVEKVSWIPSLGVTYFFGLDGISLWLVLLTTFLSVIAV
                     ICSWESVSMRLKEYYAFILMLETGMLGVFFSLDFFLFYIFWEVMLVPMYFLIGIWGSD
                     RRLYSAIKFFLYTLFGGVIMLLGILAVYFYHGAQTGVYTFDILDLMKLSYPSTPILTL
                     LGFSFSFQDLVWLAFFLGFAIKVPMFPFHTWLPDAHTDAPTAGSVILAGVLLKMGTYG
                     FIRINLPLLPESTQHFVPMIMILSIIAIIYGALVCMVQTDMKRLIAYSSVSHMGFIVL
                     GMFALNPQGVQGSIIQMVNHGLSTGALFLIVGLIYDRRHTRQISEFGGLSKQMPVYAT
                     LFAIIMFSSMGLPGLNGFIGEFLILIGAFKENYVWAAFAVTGIVLGAAYMLWLYQRTM
                     FGALENPKNADLPDLSFRELTTLVPIIVMCFWIGLYPSPFLSRTEASVNYILARVHKQ
                     QAVAEPALSDSTQLATGHIEACAEPCQSGPVLPVKGPALDNAGASRD"
     gene            complement(7170..>7542)
                     /locus_tag="VN444_05550"
     CDS             complement(7170..>7542)
                     /locus_tag="VN444_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWR21309.1"
                     /translation="DIPAALADKLRGLYNLLLNKYWVDELYEAIFIRFGKRFCGFLWG
                     FDARVVDGAVNGSGWLTMRLSVISAWNDLKIVDGLVNAIADLIQGGSVTLRRLQTGAI
                     QNYILAMALGILGMVVFYLFV"
BASE COUNT         1859 a         2232 c         2083 g         1368 t
ORIGIN      
        1 gcccctaaga gcgcacaact cagattgtga cagacccaga tctcgtaccg cccacccggc
       61 ttcagacgga acatgtcgta aaacatcgcg acctccgcca cctgggtcgg cggtaagtca
      121 agacgggcgg ccagctcacg catggccccc tctgtcagat agcctgcctc ctcctgccac
      181 aggtgcatca gcggcaacag ggcggagcgg cgatcgggat atctggacag gatgtgctgg
      241 atcgtctctt cgttcatctc atcctaaaaa gctgttcgct gacagctcat agctgatagc
      301 tgtcgtttta tcgatccacc tcgccgagta cgatatcgat gctaccgatg atcgctacca
      361 gatcggccac cagtcgcccc tctaccatcc tcgacaacga ctccaggttc acgaaggagg
      421 gtgtccgaaa atggaggcga tagggtttgt tgctgccatc gctcaccaaa tagaccccgt
      481 actcgcccct cggtgactcg atactctgat acacctctcc tgccggcacg gcaaaccctg
      541 ccgaccagag caggaagtgg tgaatgaggg cctccatgct ctgtttgatc atcggcttag
      601 gcggcggggt gagcttcgga ttagccacag cgattggccc ctccggaagg cattccagtg
      661 tctggcgaac gatagccacg ctctgctgca tctcaaagat ccggcaaagg tatcggtcgt
      721 agacatcgcc gtggattccc ctggacacct cgaaaccaaa ttgctcatag ccggaatagg
      781 gattagcctt acgaatgtcc caagggatcc cgcaggctcg gaggcttgga ccgcttaagc
      841 caagatctat ggcgtcttcc ggcttaatta caccaacccc cttggtccgt tcgatccaga
      901 tcgggttcct cgtcagcagg tcttcgtact cggccaggcg gtcaggaaag ctttcgacga
      961 aagcgtggac caccccctcg aacccctctg gcagatcggc aaacaggcca ccaatccgga
     1021 agtagctgga catcatcctg gccccggaca cctgttcgga catgtccagg atcgcctcac
     1081 gctcccgaaa ggcatagagg aagacgctca tggccccgat atcgatagca tgtgtcgcaa
     1141 gccagaccag gtggcttcca atccgggtca gctcggtcaa catgactcgg attaccttcg
     1201 cccgctgagg cacctcgatg tcaaggagct tctcgacggc caacacatag gccaggttat
     1261 tgctgagggg ggcgaggtaa tccatccgat ccgtaatggg gatgacctgc tggtaccgct
     1321 tgctttccgc gatcttctcc atcccggtgt gcaggtaccc gatatgcgga gtgcaccgga
     1381 ccacgatctc cccatccagc tccaggacca gacgcagcac gccgtgggtg ctggggtgct
     1441 ggggccccat gttgatggtc atggtgcgcc gctcagtcac ctgccgcccc ctctgcccac
     1501 catttcggcg aggactgcac cgggtagtcc ttccggagcg ggaacccctc ccattcctcc
     1561 ggcatgagta ttcgccggag atccgggtgc ccatcaaagc cgatgccgaa catatcgaat
     1621 acttcacgtt cgtgccaatt ggcggcgctc cagacggcgg tcacactggg caccgcctca
     1681 ccctcttcaa ccggcacctt caaccggatt cgccacttgt actgaaggga atagagatgg
     1741 tagaccacct cgaaccgtgg gtggtccccg agccgatcga ccgcagtcag atcagagagg
     1801 aaatcgtaca gaaggcctgg atcctctttc aggtggcgac agatacgaat gatgtcacca
     1861 ggcctgacaa ggatggtcgt ttcgttgcga aagcttctgg ccgggagggt cgcctccggg
     1921 aatctttctc gcaactgtga aactgtgcgg ttctcctcca cccctttcac gttgtccatt
     1981 ccagtccccc cctcctccag acatacagga ggccgatcag gaggatgccg agaaagacaa
     2041 tcatctcgac caacccgaat agtctcagac tattcaggat caccgcccag ggatacaaga
     2101 acacgacctc gatgtcgaag atgatgaaca acatcgcgac caggtaaaac ttgaccgaga
     2161 accgctccct ggcggagccc accgggtcga tcccgcactc gtacggagcg agcttcgcct
     2221 gtgtcggccg tcgcggcccg agtgcgtggg acacaaagag ggtagcgagg gcaaaaccgg
     2281 cgaccagaag gatcaggata aagatcggca gatacgagaa gagcactgcg tctctcccgg
     2341 gcaatcggtc atcgccctga agccttaccc tcctctatgc gcctggaacc acgtacttga
     2401 tgaagggatt cgcgaagaag atgatcaggg cgacaagcag gacatagatg gccagcgact
     2461 cgatgagcgc gagaccgata atcattgcga cctggatccg cggagctgct gccggctgcc
     2521 tcccgatcgc ctccagcgca ctcacgatgg cccggctctg tccgatcgcc gctgcgccag
     2581 aggcgatggc catggcgaat ccccccgtca ggaccgagac gacaaaaaag gttgtggcct
     2641 ctgggttagc cgcttcagcg gcaaatgcga cctgagaccc aagggcaagt agaaccgtca
     2701 ccaacagtag aacgcgcgct ccttgtgtgc gactcatcga tacgactcct ttcagaataa
     2761 agatggggtt agtggtgctc ctcgtgctca gattggaccg ctcctgcaat atacaccatg
     2821 gaaagcatca caaaaatgaa gctctggaca aagctggtaa agagactcag gggcatcatg
     2881 atacccagat agatgacata cggaatgcct aaccagatca acgacagcag gatagcgatc
     2941 acgctctcct caccgaagat attgccgaac aaccgaatcg atagagagat cggccgggcg
     3001 aggtgaccga taaactcgat ggggaacatg ataggggcca gccacagaat cggtccacaa
     3061 aagtggcgca gataggcagc gattccctgc tcccgtatgc caaagtagtt ataggagaga
     3121 aagacggtga tggctagggc cgcagtggtg ttgagattcg ccgttggagg cttgaagccg
     3181 gggacagttc ccaggagatt cccaacgaga atgaagagac cggccgtacc gatcagggga
     3241 agataccgtt tccccttggg cccgatcatc tgcgtgagca ggtccacgaa catctcgacg
     3301 actacctcca tgaagttctg catcggcccg ggcacagcat cgagccgtct cgtggccaga
     3361 tacgacacgc cgaccaggat agccatcaca acccaggcca tcgcgacatg ctccggcacc
     3421 caagctccag gaacccctaa cgcccctaga aaattaggaa tctggataag cgtaggatgg
     3481 tgctccatca cgcctccccg ttattgtgcg ggaaatcata tacacagttc aacaaatcaa
     3541 ctcccccaac gcttgaggtc ttttagaaga ttggtaaagc cagccgcaac gccgaaaata
     3601 aaaaacagaa tcgtcaacca cggcgacgtc cccagccagc gatccaaggc gataccgatc
     3661 gccgccccga tcgcaatcga agcggcaaaa gtaatcccca gtgagctcaa ccccgcgagc
     3721 tgacgccaca gtcgagcctg attgtccttc atcgttccgc cgatgaaatc tcccttggcc
     3781 ctcctttaca aaaggcgggc tgggggattt tctcttaccg cgttcggcca cactctagcc
     3841 ctctccctcc gctctgtcaa ggctccgcct gcctgcaatc aacacagacc ggcatcagac
     3901 gctgacgcct gatagctgaa tgcttactcg ctgcctacca gagaccggcg accgaggccc
     3961 tggccagctc caggaagggg cctgggtaga tcccgatagc gatggttcct gccagggtca
     4021 cgaatagcgc gacccgcaga ggccgggaag cactcaaggt caggccctgt ggcggaagat
     4081 cccgcatgta catcgccacc actaccttca tgtaatagaa caccgagatg gctgaattga
     4141 taacggcaat caccgcgagc cagatgtacc cctgttcaat ggctgcaccg aagagataga
     4201 gcttcccgac aaagcctgcg gtcgggggga cgccagccaa tgagaggaga aagatcaaca
     4261 tagcggctgc cgccataggg cttcgctggg ccaggccagt gaagtcgtcg atccgctccc
     4321 ccttcacctt gcctacgcag agaaggatca ccatagcaaa gacgccgagt gtcatcaggg
     4381 catagatcag ggtatacagc agtatggctg agatccccag ctcccgtccc gccacaactc
     4441 cgatcagcag gtacccgatg tgggcaatgg agctgtaggc cagcatccgc ttgatattac
     4501 tctgcgcaat cgctacaaca tttcccaccg tcatcgtgag caaggacagg gcgtaaaaga
     4561 gaagtgtcca gcgatcctgc agttctggga tggccaccag aaagacccgc aagaggatgg
     4621 cgagaccggc agcctctgag gccgccgcca ggagggccgc cacaggggtc ggggccccct
     4681 catacacgtc agggatgtac atgtgaaagg ggaccgccgc gatcttgaac ccgaagccgg
     4741 ccaccagcat caccataccc agaatgagcg ccggattcga gacggtgtct gctgacagcg
     4801 tagaggcaat ctctctcagg ccgatggtgc cggtaagacc atacagcatc acgatgccgt
     4861 acagcatgat cgcggaggag aaccctccca tgagcaggta cttcagggcg gcctcgccgc
     4921 ttcgccgctc ccgcttcagg aatccggcga gaatgtaaat ggaaatcgac atcgtctcca
     4981 ggccgatata gagaatcagg aggtctacag cactgaccat cagcatcatt cccagtgtgg
     5041 cgaagaggat cagcccgtag aactcccctt tgtcgattcc ttcgctcttc aggtggccga
     5101 tcgacatgag cagaatgagc gcggccgaga ccagaaatac gatcttgaag aagaacgcga
     5161 agggatcact gaggtattgc ccgctgaagg cgagctgcgt cgccccccgt tggcccatca
     5221 gggtcgtgaa ggtggcaagt acgccaagga tgctcagata tcccaaccag tctcgtccgc
     5281 ctttgggggc tatgaagtcg agcataagga ttaccatggc caccaggccc atgaccacct
     5341 ctggtgaggc agtggagatg tctaccatgt tgaacacgtc agtcacgaga cgctccggcg
     5401 ttgtccaggg caggcccctt gaccggcaag acaggcccgc tctgacaagg ctcagcgcag
     5461 gcttcgatat gtcctgtggc gagctgtgtc gaatcactca gggcaggctc ggcaacggcc
     5521 tgctgtttgt gaacccgtgc caggatataa ttcaccgacg cctccgtccg gctcagaaac
     5581 ggggaagggt agaggccaat ccagaaacac atgacgatga tcggaacgag agtcgtcagc
     5641 tccctgaaac tcaggtcggg gagatccgca ttcttcgggt tctccagcgc cccgaacatg
     5701 gtccgctggt acagccacag catataggcc gcaccgagca cgatcccggt gacagcaaaa
     5761 gcggcccaga cataattttc cttgaaggcc ccgataagaa tcaaaaactc gccgatgaag
     5821 ccgttgagtc caggaagccc catggaagaa aacataataa tggcgaacag ggtggcgtag
     5881 accggcatct gtttcgacag acctccaaac tcagagatct gccttgtatg tcgacgatca
     5941 tagatcaggc ccacgatcag gaagagggcg ccggtcgaga ggccgtggtt caccatctgg
     6001 atgatgctgc cctggacccc ttgggggttc aaggcgaaca tgcccagcac aatgaacccc
     6061 atgtggctga cagagctgta ggcaatcaac cgtttcatgt cggtctgaac catacagacc
     6121 agagccccgt aaatgatcgc gatgatcgat aggatcataa tcatcggaac gaagtgttgg
     6181 gtggactctg ggagcagcgg cagattgatc cgtatgaagc cgtaggtccc catcttcagg
     6241 aggacgccag ccagaatgac actgccggca gtcggggcat cggtgtgggc gtcaggcagc
     6301 cacgtatgaa agggaaacat cgggaccttg atggcaaaac ccaaaaagaa ggcaagccag
     6361 accagatcct ggaacgagaa cgaaaacccc agcagcgtga ggataggagt tgaggggtac
     6421 gagagcttca tcagatcgag gatgtcgaat gtgtagaccc cggtctgagc gccatgatag
     6481 aagtagaccg ccaggattcc cagcagcatg atgacgccgc cgaagagcgt atacagaaag
     6541 aatttgatgg ccgagtagag gcgacggtcg cttccccaga taccgatgag gaagtacatc
     6601 ggaaccagca tcacttccca aaagatatag aagagaaaga aatcgaggga aaaaaagact
     6661 ccgagcattc ccgtctccag catcaggata aaggcgtaat actccttcag ccgcatactg
     6721 accgattccc atgagcagat gacggcgatc acggagagga aagtggtcag caagacgagc
     6781 cacaggctga tcccatcgag gccgaaaaaa taggtgacac caagggatgg aatccatgag
     6841 accttctcta cgaactgcat ctccgcagtc gtcgagtcga agttggcggg gatccacagc
     6901 gaagccagga ggttggctat cgtgaaggcc agggcggtcc agcggacacc gcccttgcac
     6961 tccttcggga gcagcgagat gaagaacacc ccgacgaggg gcaggtaggt catcagggat
     7021 aggatcggaa actgaagaac attcatggga atgccagtcc tcctgtctta agttccaggt
     7081 tccgcgttcc gggtttcggg ttccggattc cataccgact tcgcggatct tcaactcgga
     7141 acccggaact tgaaacccga aatcttttgt tacacgaaca gatagaacac caccatgcca
     7201 aggatgccaa gcgccatggc caggatatag ttttggatag cccccgtctg caatcgacgg
     7261 agggtcacgc ttccaccctg gatcagatcc gcgatggcgt tgaccaggcc atccacaatc
     7321 ttcaggtcgt tccaggcaga aatcacactc aaccgcatgg tcagccagcc acttccattg
     7381 actgcgccgt ccacgacccg agcatcgaat ccccacagaa acccacagaa gcgcttgccg
     7441 aaacggatga agatcgcttc gtatagttca tcgacccaat atttgttcag taacagatta
     7501 tagagaccgc gcagcttgtc cgccagcgcc gcgggtatat cc
//
LOCUS       DATMXI010000294         5549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_263070, whole genome shotgun sequence.
ACCESSION   DATMXI010000294 DATMXI010000000
VERSION     DATMXI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263070"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..752
                     /locus_tag="VN444_05555"
     CDS             297..752
                     /locus_tag="VN444_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21310.1"
                     /translation="MAAFGCRDSRQGHALMQELAIVRKMLGRLEAKLLEDDTDHFPKG
                     DDQELRLALQRIGDDRLTQLMLSYAVCVRGLTAPMASRTWKQELAEEAEDLHRPLVSR
                     LQRLERLARSHAGRLWLRLQARLYRVFHQSPHPQRLPSPKENGNRGQLL"
     gene            752..1447
                     /locus_tag="VN444_05560"
     CDS             752..1447
                     /locus_tag="VN444_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWR21311.1"
                     /translation="MGKLIRWLIVFPLLGLLLYVGHPFLLRAMGRYLITEDRLQKAGA
                     IAVLAGDGGVGRTLEAVRLYQDGYAPRIVLTHQRLPKGYESLARLGITVPEERDIQWM
                     VLKSMRIPATVVLRVSERADSTEREMVYLARLLKEYRIETVILVTDRSHSTRASKILA
                     RISGGRIAIISRPTRYDTFDPEGWWRSRADAKEVLLEYEKLINYLVQSASAGLTGWRK
                     GGSDSAVPAEGTF"
     gene            1513..1974
                     /locus_tag="VN444_05565"
     CDS             1513..1974
                     /locus_tag="VN444_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWR21312.1"
                     /translation="MQEKRGMFDSIWGALGPTSPEPTQQAPMMEMSTSAFIVSEKIQI
                     RGDLTGEGDIQLMGGFFGTIDLVGTVVIGESAQVEADIAATNIRVGGHVKGNLIASGR
                     VDLLPTGSVTGNVKTGSIAAAEGASLKGEIEINRRQAQGQMEEEPDRFSRA"
     gene            2052..4640
                     /gene="clpB"
                     /locus_tag="VN444_05570"
     CDS             2052..4640
                     /gene="clpB"
                     /locus_tag="VN444_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWR21313.1"
                     /translation="MRFDKFTFKAQEAIQQAQKLAEEREHQAIDVEHLLLALIDQAEG
                     VVQPILRKMGATPGQVASRLIEELRRLPKVSGLPQGQVHITPRLEKVLNAALSEAERL
                     KDEYVSTEHLLLAISDEGGGAVGKILREAGITKDRIYAALQEFRGSQRVTDQTPEEKY
                     QALERYARDLTDLARKGKLDPVIGRDDEIRRVIQVLARRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPESLKNKRVMALDIGALVAGSKYRGEFEDRLKAVLREVTEQEGQIV
                     LFIDELHTLVGAGAAEGAMDASNMLKPALARGELRCVGATTLDEYRKRVEKDAALERR
                     FQPVLVGEPSVEDTISILRGLKERYEVHHGVRIKDSALVSAAVLSNRYIADRFLPDKA
                     IDLIDEAASRLRMEIDSMPTELDELSRRARQLEIEREALKKETDPESQERLERLADEL
                     TEIRAKEEGLKARWEQEKGFIQRIRSLKERIEAAKLQEQVAERQGELGKVAELRYGMV
                     ASLQKEMDAEKRGLEQVQQDGAMLKEEVDEEDVAEVVSKWTGIPVTRMLEGETQKLLT
                     MEDKLRERVVGQYEAVTAVSDAIRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFLFDDERAMVRLDMSEYMEKHTVSRLIGAPPGYVGFEEGGQLTEAVRRRPYCVLLLD
                     EIEKAHTDVFNILLQILDDGRLTDGQGRTVDFKNTIIIMTSNLGSQVIQELGGRDDEE
                     MRRRVLEAVRTHFRPEFLNRVDEILIFHTLSLAQIKAIVEIQLKRVGKRLAERKLDLE
                     VTDAAKDFLAKEGYDPVFGARPLRRAIQRVLLDPLARRLLEHEFAEGDTVRVDAARGE
                     LILSKD"
     gene            4802..5275
                     /locus_tag="VN444_05575"
     CDS             4802..5275
                     /locus_tag="VN444_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HWR21314.1"
                     /translation="MAPVAYIEQIRRLYSSEPPYQWTVNLTSPWTPMVKPVRECRIAL
                     ISSGGIYHPSQEPFNPVKNDLTFREIPKTADLADLRISHYSKNARDVKDLNTIFPLGR
                     FRELEAQGRIGELASVAFTFMGRIFTRTRLQEEMAPHLIGRLKALQVDAAFLVPV"
     gene            5294..>5549
                     /locus_tag="VN444_05580"
     CDS             5294..>5549
                     /locus_tag="VN444_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HWR21315.1"
                     /translation="MGLFARAIEEAGIPTVMLSAALDITSSVKPSRAVFVNFPLGHQA
                     GKPCDPEGQTRIVLDALDILETATTPGTLIQLPYKWDERDP"
BASE COUNT         1166 a         1526 c         1826 g         1031 t
ORIGIN      
        1 gcaccaggcc tcattcatgc ggtgttccgc ttggtaacgg acgatctcgc cgatcgtttt
       61 cgaaaccctg agggcagcga acgaagaggg atcgaaaagg ccggccttca aggaagaggt
      121 tgtcatggtt acgtatccac ccagaggcct atttgaatcc agcgctgaca ggtagcgtga
      181 tactctttct gaggtgttga aatgaggttg actaagcttg catagcatac tatcctcggc
      241 ctggcaagca cttgtcacaa aagaacacac catgcgacca catgggaagg gtggtgatgg
      301 ccgcgttcgg gtgtcgtgat tcgcggcagg gtcatgcgct gatgcaggaa ctcgccatag
      361 tccgaaagat gctcggccga ctcgaagcca aactgctgga ggacgatact gatcatttcc
      421 caaaggggga tgatcaggaa cttcgcctcg cactgcagcg tattggtgat gaccggttga
      481 ctcaactgat gctgagttat gccgtctgtg tccgggggct cactgcgccg atggcatctc
      541 gtacctggaa acaggagctg gcggaggagg cggaggatct ccaccgtccc ctcgtgagcc
      601 gcctgcagag gctagagcgt ctggcccggt cccacgcggg acgtctgtgg ttgcggttgc
      661 aggcccgtct ttaccgtgtc tttcatcagt ctccccatcc acagcgcctg ccttcaccca
      721 aggagaatgg caaccgtggg cagttgcttt gatgggaaaa ctgatccggt ggctgatcgt
      781 tttcccgctc cttggccttc tgctctacgt cggacaccca tttctcctga gagccatggg
      841 ccgctacctg atcacggagg atcgtctaca aaaggccggc gcgatcgcgg ttctggcagg
      901 agacggaggc gtcggccgca cgctcgaggc tgtcagactt taccaggacg gctatgcccc
      961 aaggatcgtc ctgacccatc agcgcttacc gaaagggtac gaatcgctgg cacggctggg
     1021 cattaccgtc ccggaagagc gggatatcca atggatggtc ctcaagtcga tgcggatccc
     1081 tgccaccgtc gtcctccggg tcagtgagcg ggccgatagt accgagaggg agatggtcta
     1141 tctcgctcgc cttctcaagg agtaccggat agaaacagtc attcttgtca cggacagatc
     1201 gcattcaacg cgggcaagta agattctggc ccgcatctcc ggtgggagaa ttgcgatcat
     1261 cagccggccc acccgctacg atacctttga tccggaaggg tggtggcgct ctcgcgctga
     1321 cgcgaaagag gttctcctgg aatacgagaa gctaatcaac taccttgtgc agtcggcgag
     1381 tgccggcctc accggctggc ggaaaggcgg gagtgattcg gccgttccgg ccgagggtac
     1441 attctgacca tcaggatctc aggtctggtc gctgcgagag ggttaacctg ttcatggatg
     1501 gagggacatg ccatgcaaga gaagcgaggg atgtttgact caatttgggg ggcattggga
     1561 ccgaccagcc cggaaccgac acagcaagct ccaatgatgg agatgtcgac gagtgctttt
     1621 atcgtcagcg agaagatcca gattcgcggg gatctgactg gcgaaggcga cattcagctc
     1681 atgggtggat tttttgggac cattgatctt gtgggaacgg ttgtgatcgg agagtcggct
     1741 caagtagagg ccgatatcgc tgcgaccaat attcgtgttg gcggccacgt gaaaggtaat
     1801 ctgatcgcca gtggacgggt cgatctcctg ccaaccggca gcgtaacggg taacgtgaag
     1861 accggcagca tcgccgccgc agagggtgcc tccttgaagg gcgagattga gatcaatcgc
     1921 cggcaggccc aaggccagat ggaggaggag cctgatcggt tcagcagagc ctgagaagcc
     1981 tcgttgcgct cagcctttag ccgtacgcgc gtcgaggagg tacggggcga atagggggac
     2041 gtatggcctt tatgcggttt gataaattca cctttaaggc gcaggaggcg atccagcagg
     2101 ctcagaagct ggccgaggag cgggagcatc aggcgattga cgttgagcac ctgttgctgg
     2161 cgttgatcga tcaggcggaa ggggtagttc aaccaatctt gaggaagatg ggggcaaccc
     2221 ctggccaggt cgcttcacga ctcatagagg agctgcggcg gctccccaag gtctccggct
     2281 tgccacaagg tcaggtccat atcacgccac gcctggaaaa ggtattgaac gctgcgctgt
     2341 ccgaggcgga acggctgaag gacgagtatg tcagtaccga gcatctgctc ctggcgatct
     2401 ccgacgaggg cggcggcgct gtcgggaaga tcctgcgcga ggccgggatc acgaaagaca
     2461 ggatctacgc ggccttgcag gagttccgcg gcagccagcg cgtcaccgac cagactccag
     2521 aggagaagta ccaggcgttg gaacgatacg cccgcgatct gaccgatctg gccagaaaag
     2581 ggaagctgga cccggtcatc ggtcgggacg atgaaatccg ccgggtgatc caggtcctgg
     2641 cgcgccggac caagaacaac ccggtcctga tcggtgagcc cggagtgggc aagactgcca
     2701 tcgtggaagg actggcgcag cggatcgtta acggggatgt gccggagtcg ctgaagaaca
     2761 agcgcgtcat ggcgctggac atcggcgcgc ttgtggccgg gagcaagtac cgtggggagt
     2821 ttgaggatcg gctgaaggcg gtcctgcgcg aggtcactga gcaggagggg cagatcgtcc
     2881 tcttcatcga cgagttgcac acccttgttg gggccggcgc tgctgaaggg gccatggacg
     2941 cctcgaacat gctcaagcct gcgctggcgc gaggtgagct tcgatgcgtg ggagctacga
     3001 cactggacga ataccgcaag cgggtagaga aggacgctgc gcttgagcga cgcttccaac
     3061 cggtcctggt cggtgagccg tcagtcgagg ataccatctc gatcctccgc ggcctgaagg
     3121 agcggtacga ggtccaccat ggcgttcgga tcaaggactc ggcactggtg tctgccgcgg
     3181 tcctcagcaa ccggtacatc gccgaccggt ttctgccgga caaggcgatc gacctgatcg
     3241 atgaggcggc gtcacgcctc cggatggaga tcgacagtat gccgacagag ctggacgaac
     3301 tcagtcggcg ggccagacag ctcgagattg aacgtgaggc cctgaagaag gagaccgatc
     3361 cggagtcgca ggagcgcctg gagcgtctcg cagacgagct cacggagatc cgggccaaag
     3421 aggaagggct caaggctcgc tgggaacagg agaagggatt catccagcgg atcaggagcc
     3481 tcaaggagcg gattgaggcg gcgaaactcc aggagcaggt ggccgagcgg cagggagagc
     3541 tggggaaggt cgccgagctc cgctatggca tggtcgcctc tctacagaag gagatggacg
     3601 ccgaaaagcg cggcctcgag caggtccagc aggacggcgc catgctcaag gaggaggtcg
     3661 atgaggaaga cgtcgccgaa gtcgtctcca agtggaccgg gattccggtc accaggatgt
     3721 tggaggggga gacgcagaag cttctgacca tggaggacaa gctcagggag cgcgtggtgg
     3781 ggcagtatga ggcagtcacc gcggtgagcg atgccatccg tcgcgcccgc tcggggctcc
     3841 aggacccgaa tcggccgatc ggctcgttca tcttcctggg gccgacgggg gtggggaaga
     3901 ccgagctggc ccgggcgctg gccgagttcc tgttcgacga cgagcgcgcg atggtccgcc
     3961 tggacatgag cgagtacatg gagaagcata ccgtttcccg tcttatcggt gcgccgccgg
     4021 gctacgtcgg gttcgaggag gggggacagc tcactgaggc ggttcggcgt cgcccctact
     4081 gcgtcctgct cctggacgag atcgaaaagg cccacaccga tgtcttcaat atcctgctgc
     4141 agattctgga tgatggacgg ctgaccgatg ggcaggggcg aaccgtggac ttcaaaaaca
     4201 cgatcatcat catgaccagc aacctgggga gtcaggtcat tcaggagtta ggcggtcgcg
     4261 atgatgagga gatgcgtcga cgagtgctgg aggccgtaag aacccacttt cggccggagt
     4321 tcctgaaccg agtagacgag atactgattt tccacacgct ctcactggct cagatcaaag
     4381 cgattgtgga gatccagctc aagcgggtgg ggaagcggct tgccgagcgg aagctcgatc
     4441 ttgaggttac ggatgcggca aaggactttc tggccaaaga aggatacgat ccggtctttg
     4501 gggcgagacc gctcaggcga gccatccagc gagtgctgct ggatcccttg gcgcgtcgtc
     4561 tgttggagca tgagtttgcc gaaggcgaca ccgtaagggt cgatgcggct cgcggtgagt
     4621 tgatcctgag caaggattga cggaagcgca tcgaatgaca cgtcatcgct acgaaagccc
     4681 cttgggttat tgcgaggcga agccgaagca atctcacagt ccttcaagac aacgacggtg
     4741 ggattgccgc gctcccgttg gtcgctcgca atgacgatct gaaggagaga ggggagggaa
     4801 gatggcgccg gttgcctaca tcgagcagat ccggagactc tactcgagcg agccgcctta
     4861 ccagtggacg gtgaacctga cgagtccctg gaccccaatg gtcaagccgg tccgagagtg
     4921 ccggatcgcc ctgatcagct ccggggggat ataccacccc tcgcaagaac cgttcaaccc
     4981 cgtcaagaat gacctgactt tcagggagat cccgaaaaca gccgatctgg ccgacctccg
     5041 aatctcccac tactccaaga acgcccggga cgtcaaagac ctcaatacga tctttcccct
     5101 cggacggttc agggagttgg aggcgcaagg acgtatcggt gagctggcct ctgttgcctt
     5161 caccttcatg ggacggatct ttacgcggac caggctccag gaagagatgg cgccgcatct
     5221 catcgggcgt ctgaaggcac tgcaggttga tgcggccttc ctggttccgg tctgacagtt
     5281 gtgccaccag tccgtgggac tgttcgcccg cgccatcgag gaggccggca ttccaaccgt
     5341 catgctttct gcggcccttg atattacctc ctcggtcaag ccgtcccgcg ccgtcttcgt
     5401 gaactttccc ctcggtcacc aggccggcaa gccatgtgac ccggaggggc agacccggat
     5461 cgttctcgac gcacttgata ttctggagac ggcgaccact cccggtaccc tcatccagct
     5521 tccctacaag tgggacgagc gcgatccgc
//
LOCUS       DATMXI010000295         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_164614, whole genome shotgun sequence.
ACCESSION   DATMXI010000295 DATMXI010000000
VERSION     DATMXI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164614"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(866..1636)
                     /locus_tag="VN444_05585"
     CDS             complement(866..1636)
                     /locus_tag="VN444_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWR21316.1"
                     /translation="MGHWGRRSLSWLGELAVAVIIVGLVGASSAMAEDPQSTGAVAAQ
                     QVEGAVMLETEQIEEYDPWEPYNEKIFDFNHNVFDRYVLKPVATTWDYLPDPVQESLG
                     NAFDNIAMPRRVVNNLLQAKFKGAGTELARFGINTTVGIVGLFDVAKTYGLEKSDADT
                     GQTMGIWGVGPGPYLILPFLPPLTARDAFGFAADVAMDPLNYLIPFAALAGRGTGDVV
                     NTRSQNLELFESVEESTVDLYSAVRNGYLQRRHRAIAK"
     gene            complement(1839..>2575)
                     /locus_tag="VN444_05590"
     CDS             complement(1839..>2575)
                     /locus_tag="VN444_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWR21317.1"
                     /translation="PPPPVVLSERFPRDRLHGWKFIRFGPDGWLYIPVGAPCNVCERE
                     DKRYASITRMKPDETRLEIFARGVRNTVGFDWHPKTRELWFTDNGRDWMGDDLPPDEL
                     NHAPRPGLHFGFPYCHGRTLPDPRFGKGRTCQEFTPPAVELGPHVAALGARFYTGTMF
                     PDEFRNHLFIAEHGSWNRSAPIGYRVTLVRLENQRTLPYEVFAEGWLQGGHAWGRPVD
                     LLVMPDGAMLVSDDKADVIYRISYRK"
BASE COUNT          589 a          738 c          706 g          542 t
ORIGIN      
        1 gcccaggtgg cagcgaatcc cgcgagcaga agcagggggg tccctaggcg agttatcttt
       61 cgactggagg atggcatctg cccactcatc gctatcgtaa tcgaatccag ttgtaggaga
      121 gggggacgat ggcaatctga agcgcacgtg caacgtcaat acggatggca actatcgtgc
      181 catgttgagc actgggagat gatgctcgga aaagcgccca cgcgacgcac agttgaccgt
      241 gccgcactca gggtggttcg atctcctgag agttggaatt gcgtgcgagg agagtaaatt
      301 tttctcgctg ttcaacgaga aaactgcata gcggtcacgt aactactcag gtagatagcc
      361 tgtaggctgt tagactgtag gaacgcgcac agatgagtca ggcgttttcc gaatgcgacg
      421 cgacaatgat ggagaccgaa aaggttctgg acgccttgat tcggtccgtg cgcaacgact
      481 aacagcctgc agcctacagc ctaaatacct gagtagttac acgatcaatg ccaatgggtt
      541 aggagacgtg acccgtgtct gtcggaggtc gagaagggag acctcaagaa agagattctt
      601 ccggctgtta cgtgggtcct tcggtgtggg cgtgagtagg caggaggcgg aacggatact
      661 tcgacgaagc tcagtacagg cttcgccgaa ggagcggggg cccactgaga cgagaaggtc
      721 gcccagaggg gcgtagaaac gacgactatc tgctacgctc taaagcgcat ccgggatatt
      781 gtttactcac acaagatccg gaagagctga cagtggtgac agccgcttta cgtcacaagg
      841 ccgtgagaga gttgaatgta tacgattact tcgcgatggc ccggtgcctc cgctgcaggt
      901 agccattccg caccgcactg tacaagtcaa cggtcgactc ttcaacgctc tcgaacaatt
      961 ccaggttctg cgacctggta ttgacgacgt ccccggttcc ccttccagcc aatgcagcga
     1021 acgggatgag gtaattcagt gggtccatgg ccacgtccgc cgcaaagcca aaggcgtcac
     1081 gggccgttag cggcggcaag aagggcaaaa ttaaataggg accggggcct actccccaga
     1141 tacccatcgt ttggccggta tcggcgtcgc tcttttcgag gccataggtc ttcgctacat
     1201 cgaagagacc gacgatgccg actgttgtgt tgatgccgaa gcgagccagt tcggttccgg
     1261 cccccttgaa tttggcctgc agcaggttat tgactacacg gcgaggcatg gcgatgttat
     1321 cgaaggcgtt accgaggctc tcctgcacag ggtccggcag ataatcccat gtagtcgcca
     1381 ccggtttcag gacatatcga tcaaacacat tgtggttgaa gtcgaagatc ttttcgttgt
     1441 aaggctccca ggggtcgtat tcctctattt gctctgtctc cagcatcacc gcgccttcca
     1501 cttgttgggc agcgactgca ccggtcgatt gcggatcctc tgccattgcg ctcgacgcgc
     1561 cgaccaagcc tacgattatc actgcaacgg ccaattcacc caaccacgat aacgatctcc
     1621 tgccccagtg tcccaccctt tctctccttt ccttggtcaa agccgatgtt cgcgattgct
     1681 tatcgacacc acttccctgt tgcctcacct tcgccgctcc tcaaaaaggc ctatcatctt
     1741 taccataaat tgtctttctt gtcgcgcaaa aatatgcagc aaaaatatac aacccgcgca
     1801 atcgcccgaa agccttcctt gctaaaatat gagataggtc attttctgta gctgatccga
     1861 taaattacat ctgccttatc gtccgaaacc agcatcgcgc catcaggcat gacaagcagg
     1921 tccacaggac gtccccacgc gtgaccgcct tggagccacc cctcggcgaa gacctcgtac
     1981 gggagcgtgc gctggttttc aagtcgaaca agggtcacac gatacccgat cggcgcgctc
     2041 cgattccacg atccgtgttc cgcaatgaac aggtgatttc gaaactcgtc cggaaacatc
     2101 gtccccgtgt aaaagcgcgc gccgagcgct gccacgtgcg gcccgagttc aaccgctggt
     2161 ggggtgaact cctgacacgt tcgccccttc ccgaacctgg gatcgggaag agttcgccca
     2221 tggcaatagg gaaagccaaa gtgcagccct ggacgcggcg catgattcag ttcatccggt
     2281 ggcagatcgt cgcccatcca gtctcgcccg ttatccgtaa accagagctc acgcgtcttc
     2341 ggatgccaat cgaacccaac cgtattgcgg acgccccgcg cgaagatctc caatcgagtc
     2401 tcatccggct tcatgcgcgt gatcgacgcg tagcgtttat cttcgcgctc acagacatta
     2461 cacggagctc cgacggggat gtagagccac ccatccggac caaagcgaat gaacttccag
     2521 ccatgaaggc gatctcgagg gaagcgctca ctgagcacta caggcggggg cgggt
//
LOCUS       DATMXI010000296         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_199264, whole genome shotgun sequence.
ACCESSION   DATMXI010000296 DATMXI010000000
VERSION     DATMXI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199264"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="rplN"
                     /locus_tag="VN444_05595"
     CDS             <1..238
                     /gene="rplN"
                     /locus_tag="VN444_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWR21318.1"
                     /translation="EAVPEGSVKKGEVVKAVVVRTAKEMRRKDGSYIKFDRNAAVLLN
                     EQNNPIGTRIFGPVARELREARFMKIISLAPEVV"
     gene            266..598
                     /gene="rplX"
                     /locus_tag="VN444_05600"
     CDS             266..598
                     /gene="rplX"
                     /locus_tag="VN444_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWR21319.1"
                     /translation="MMAVGQGLHVKKNDLVAVIAGKDKGKRGKVLKVIPKTMRALVEK
                     VNLVKRHTKPGATSKQGGILERENPMHASNLMLVCNKCDRPVRVGVSRLADGKKVRVC
                     KKCGETVG"
     gene            681..1223
                     /gene="rplE"
                     /locus_tag="VN444_05605"
     CDS             681..1223
                     /gene="rplE"
                     /locus_tag="VN444_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWR21320.1"
                     /translation="MPRLRERFRDVIAPALMKQFRYKNIWQVPRPSKVVINMGLGEAV
                     ANVKVIDIAIEELSVVTGQKPIVTRARKSEAGFKLREGIPIGCKVTLRGDRMYEFLDR
                     FVSVALPRIRDFRGVPAKSFDGRGNYNLGVREQLIFPEIKYDKVEAVRGMDIAIETSA
                     KTDEEARALLEHFGFPFQKS"
     gene            1318..1503
                     /locus_tag="VN444_05610"
     CDS             1318..1503
                     /locus_tag="VN444_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWR21321.1"
                     /translation="MAKLSLILKSQRQPKFQVRGYHRCRACGRPRGYIRKFEMCRICF
                     RDLALRGEIPGVIKASW"
     gene            1591..1986
                     /gene="rpsH"
                     /locus_tag="VN444_05615"
     CDS             1591..1986
                     /gene="rpsH"
                     /locus_tag="VN444_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWR21322.1"
                     /translation="MMTDPIADMLTRIRNANLVVHDKVHIPASKVKVEIAKVLREEGY
                     IRNFKLLDVDNRRVLRIYLKYGPGDQRIITGLRRVSRPGLRIYATSRRLPRVMNGLGI
                     AILSTSHGIMAERSARDRGAGGEVLCYVW"
     gene            2077..2613
                     /gene="rplF"
                     /locus_tag="VN444_05620"
     CDS             2077..2613
                     /gene="rplF"
                     /locus_tag="VN444_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWR21323.1"
                     /translation="MSRIGRKLIPLSDQVTVEISRGSIHVEGPKGKLSLRLHPSMTVK
                     LEDRQLHCVRPSDNKLHRSLHGLTRTLIANMVEGVTKGFEKKLEMIGVGYRASVQGRN
                     LALMLGYSHPLILPLPEGINVSVESQNLLTISGVDKQAVGEVAAKIRSLRPPEPYKGK
                     GVKYTGERIRRKAGKTGA"
     gene            2647..3015
                     /gene="rplR"
                     /locus_tag="VN444_05625"
     CDS             2647..3015
                     /gene="rplR"
                     /locus_tag="VN444_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWR21324.1"
                     /translation="MTGDGGKQERRLRRHRRIRKRIVGSAACPRLCVFRSARHIYAQI
                     VDDEQGKTLAAASTLSREIREKEQVGDKTAAARLVGELLASKALAAHISRVVFDRGGF
                     KFHGRVKALAAGLGEKGVRI"
     gene            3135..3644
                     /gene="rpsE"
                     /locus_tag="VN444_05630"
     CDS             3135..3644
                     /gene="rpsE"
                     /locus_tag="VN444_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWR21325.1"
                     /translation="MKQIKADALNLAERIVHINRVAKVVKGGRRFHFSALVVVGDQNG
                     HVGIGLGKAHEVPEAIRKGIEAAKKELIGIQLQRTSIPHEVVGRYGAAKVILRPASQG
                     TGVVAGKAARAVLEAVGVRDVLSKSLGSDNPHNVVKATLKGLQLLRSPEEVARARGVR
                     QEGLVAQGA"
     gene            3646..3846
                     /gene="rpmD"
                     /locus_tag="VN444_05635"
     CDS             3646..3846
                     /gene="rpmD"
                     /locus_tag="VN444_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWR21326.1"
                     /translation="MDKGLRIILRKSKIGHPAHQARVLAGLGLRKINGSVVRADTPTI
                     RGMIRKVVHLVDVQQVDSEEKG"
     gene            3843..4289
                     /gene="rplO"
                     /locus_tag="VN444_05640"
     CDS             3843..4289
                     /gene="rplO"
                     /locus_tag="VN444_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWR21327.1"
                     /translation="MKLHELKPPAGASQRRKRVGRGTGSGHGKTSGRGEKGQKARSGA
                     HIHPWFEGGQLPLHRRVPKRGFTNKFRKVYATVNLKDLERFEAGTTVTPGLLQERRIV
                     KDLKVGLKVLAEGVLSKPLNVAAHKFSKRSIEKILASGGMVEVISA"
     gene            4286..5596
                     /gene="secY"
                     /locus_tag="VN444_05645"
     CDS             4286..5596
                     /gene="secY"
                     /locus_tag="VN444_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWR21328.1"
                     /translation="MTDWAGNIFRVPELKKRIIFTALALIAYRLGSHIPTPGIDAHAL
                     LSFFQQAGGTLLGFFDLFSGGALRRLSILALGIMPYISASIILQLLAVVFPPLEKLSK
                     EGEAGRKKISQYTRYGTVLLAAIQAMGIAIGLENMRSPTGEQIVVDPGVVFRLMTTIT
                     LTTGSIFLMWLGEQITERGIGNGISLLIFAGIIVRVPEAIVNTWRLLTTGELQALVLL
                     IVLALMVLVVAGVIIMTLGQRPIVVQYAKRIVGRKIYGGQSTHIPLRINTAGVIPVIF
                     ASSIIVFPATIAQFINHPWMQGVARALSPGTVTYTALYAVAIIFFTYFYTAIVFNPTD
                     VADNMRKHGGFIPGIRPGSRTAEFIEKVLDRITLVGAIYLTLISILPELMITTMSVPF
                     FFGGTSLLIVVGVALDTVQQVESHLLMRNYEGFLKKGRVKGRLG"
     gene            5604..6251
                     /locus_tag="VN444_05650"
     CDS             5604..6251
                     /locus_tag="VN444_05650"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWR21329.1"
                     /translation="MRLVLLGPPGAGKGTQARLLTAKSDVVHVSAGDLLRQAVADGSE
                     LGQKAKSIMAQGSLVPDGVVTGIIEERLRRPDCVSGYILDGFPRTLRQAEALSDVLRL
                     LQAPLDWVISVEVSEDDLVRRLAGRRICRACGAMFHVDTKPPTQHGICDSCGDQLFQR
                     DDDKEDTIRHRLRVYREQTEPLIAYYHTMGLLRRINGQGTIEEIAQRIHQALGDG"
     gene            6260..7039
                     /gene="map"
                     /locus_tag="VN444_05655"
     CDS             6260..7039
                     /gene="map"
                     /locus_tag="VN444_05655"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWR21330.1"
                     /translation="MMVLKSPWEIDLMRKSSRLVAETLGRLTRLIEPGLTTLELDRAA
                     EAYILRRGGKPAFKGYRGYPYTLCASVNEQVVHAFPSARKLEEGDIVSLDLGVIVDGY
                     YGDAAITVPVGKVSEGARRLIAATREALTRATMAALPGNHLSDISHAVQSVVEARGFS
                     VVRLFVGHGIGRSLHEEPQIPNFGPPAQGPMLKPGLVLAIEPMANAGGPDVMILDDHW
                     TAVTCDSSLSAHFEHTVALTEDGVEVLSCLTEDESPQGGGR"
     gene            7039..7257
                     /gene="infA"
                     /locus_tag="VN444_05660"
     CDS             7039..7257
                     /gene="infA"
                     /locus_tag="VN444_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWR21331.1"
                     /translation="MPKEEAVEVEGTVVEPLPNAMFRVELETGHKVLAHISGKMRMHF
                     IRILPGDKVIVELSPYDLTRGRITYRYK"
     gene            7312..7425
                     /gene="rpmJ"
                     /locus_tag="VN444_05665"
     CDS             7312..7425
                     /gene="rpmJ"
                     /locus_tag="VN444_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWR21332.1"
                     /translation="MKVRASVKPMCEKCKVIRRKRVLRVVCDNPRHKQRQG"
     gene            7521..7895
                     /gene="rpsM"
                     /locus_tag="VN444_05670"
     CDS             7521..7895
                     /gene="rpsM"
                     /locus_tag="VN444_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWR21333.1"
                     /translation="MARIAGVDLPREKRLEIALTYIFGVGRAASHKILQDSGVTPDVR
                     VKNLTEEEIAKLRRTIEGNYRVEGDLRREISLNIKRLMDIGAYRGLRHRRGLPVRGQR
                     TSTNARTRKGPRRTVGMKSKKV"
     gene            8014..8424
                     /gene="rpsK"
                     /locus_tag="VN444_05675"
     CDS             8014..8424
                     /gene="rpsK"
                     /locus_tag="VN444_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWR21334.1"
                     /translation="MAEEVKPRRPAGRTGGRKETKNIAHGIACVQATFNNTIVTITDT
                     TGNVVAWASAGSVGFKGSRKSTPFAAQRAADSAAQRAMSQGMKEVKVYIKGPGAGREA
                     AIRALQAAGMEISTIKDVTPIPHNGCRASKRRRV"
     gene            8542..>9072
                     /gene="rpsD"
                     /locus_tag="VN444_05680"
     CDS             8542..>9072
                     /gene="rpsD"
                     /locus_tag="VN444_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWR21335.1"
                     /translation="MARYLDSACKLCRREGLKLFLKGDRCLSTKCAIEKRNFIPGVHG
                     QRRTKVSDYCRQLREKQKMRRIYGVLETQFRIYYRLAERQTGITGENLVKILEQRLDS
                     VVHRLGFAASRAQARILITHGHILVNGRKTDIASYRVRPGETIEVRPKSREIAAIKTA
                     FEVAKRRTLPSWLELDP"
BASE COUNT         2063 a         2127 c         2898 g         1984 t
ORIGIN      
        1 agaggcggtc ccggagggtt cggtgaagaa gggcgaggta gtaaaagcgg tggtggtcag
       61 gacggcgaag gagatgcggc gcaaagacgg ctcttacatc aagttcgatc gaaacgctgc
      121 cgtactcctg aacgagcaga acaacccgat tggtaccaga atctttggtc cggttgcccg
      181 agagctgcgt gaagctcggt tcatgaagat tatctccctg gcgccagagg tcgtgtaaga
      241 tggcgctggc tgaatctgtg agggcatgat ggcggtggga caggggcttc atgtcaagaa
      301 gaatgatctg gttgccgtaa tcgctgggaa ggacaagggg aagcggggta aggttctgaa
      361 ggtgatcccc aagaccatgc gggccttggt ggaaaaggtc aatctcgtca agcggcatac
      421 caagccgggg gcgacctcaa agcagggggg aatccttgag cgtgagaacc ctatgcacgc
      481 gtcgaatctg atgttggtgt gcaataaatg cgaccgccct gtccgtgtcg gcgttagtcg
      541 cctcgccgac ggcaagaagg tgcgagtttg taaaaagtgt ggcgagacag ttggttaggg
      601 gaagattgat ggcagacgag agcaaaaaca agaagaaaga gaagtcggac gtgaaagagt
      661 ctcgcgtgga gtctcgcgcc ctgccacgac tccgcgagcg ctttcgtgat gtgatcgctc
      721 ctgcccttat gaagcagttc aggtataaga atatctggca ggttccgcgc ccctcgaagg
      781 tcgtcatcaa tatgggactg ggagaggctg tagctaacgt gaaggtgatc gacatcgcga
      841 tcgaggagct ctcggtcgtg acgggacaga agccaatcgt cacgcgcgcc aggaagtctg
      901 aagcaggttt caagcttagg gaggggatac ctatcggttg caaggtcacc ctgcgcggag
      961 ataggatgta cgagttcctc gatcggttcg taagtgtcgc gctgccacga attcgtgact
     1021 tcagaggcgt gccggcgaag tcgttcgatg gccgtggtaa ttacaatctg ggtgtgaggg
     1081 agcaacttat cttcccggag atcaaatacg ataaggtgga agcagttcgg ggtatggata
     1141 tcgccattga gacttcagct aaaacggatg aggaggcgcg cgcgctcctc gagcatttcg
     1201 gtttcccctt tcagaagagc tagcggcaca aagagcggtc agctatcagc gaagacatgt
     1261 cgcgcgtttg tgttattgaa agctgactgc tgattgctgg aagccaggga gtaacgtatg
     1321 gcaaagcttt cactaattct taagagccaa cggcagccaa aattccaggt acgcggctat
     1381 caccgctgtc gtgcctgcgg gcgaccgcgc ggttacatta ggaagtttga gatgtgtagg
     1441 atctgctttc gggatctggc ccttcgtggt gagattccag gcgtcatcaa agcgagttgg
     1501 taggaatgtt atggccctca ggctcgaagc cttgagctct cttggcgtcg aacctcgaac
     1561 actaaacgtg gaacttggag taagccgacc atgatgaccg atcccatagc ggacatgctg
     1621 accaggatca ggaacgcgaa cctggtcgtt cacgacaaag tccatatccc cgcctccaag
     1681 gtgaaggttg agattgccaa agtcctacgg gaggaggggt atatcaggaa cttcaaactt
     1741 cttgacgtcg acaaccggcg ggtgcttcga atctacctga agtatggtcc gggggaccag
     1801 cggatcatta ctggtcttcg ccgagtgagt cggccaggac ttcggatata tgcgacgtcg
     1861 cgacggctcc ctcgggtcat gaacggtctc ggtattgcga ttctttcgac ctcgcatggg
     1921 atcatggcag agcgttcggc cagagaccgc ggagcaggtg gtgaggtgct gtgctacgtc
     1981 tggtaaaggg gtcgaggttt acgggagggg ctgcgaagag ttagggcgag tgtcagaagc
     2041 attggagcgt tgcgctacag acggaatagg tagggtatgt cacgaatcgg aagaaagtta
     2101 atacctctct cagatcaggt gacagtagag atttcgagag gcagtatcca tgttgagggg
     2161 ccaaagggga agctatcgct tcgcctccat ccctcgatga cggtgaaact ggaggaccgc
     2221 cagcttcatt gcgtgcggcc ctctgacaat aaactccatc gttcgcttca tgggctcact
     2281 cgtacgctga ttgccaatat ggtagagggg gtcaccaagg gatttgagaa gaagctggag
     2341 atgatcgggg ttggctatcg tgcatcggta caagggagga atctcgcgct gatgttgggc
     2401 tattcgcatc ctctcattct tccgctgcct gaagggatta atgtttcagt ggagagtcag
     2461 aatctgctga cgatctctgg agtagataaa caggcggtcg gagaagtggc cgccaagatt
     2521 aggagtctca gaccgcctga gccatataag ggcaagggag tcaagtacac gggtgagcgt
     2581 attcgccgta aggccgggaa gactggggcg tagagcggat tacgtatctc ggggggggga
     2641 tttgagttga ctggagacgg gggaaaacag gagcgtcggc tacggcgcca tcgccgaatc
     2701 aggaagcgca ttgtcggcag cgcagcttgt ccgcgactgt gtgtatttcg gagcgctcgc
     2761 catatttacg cccagattgt cgacgacgag caggggaaga cgcttgctgc cgcttccacc
     2821 ctttcgaggg agatcagaga aaaagagcag gtgggggaca agactgctgc cgctcggtta
     2881 gtcggtgagc tattggcgag taaagccttg gcagctcata tttctcgggt ggtctttgac
     2941 cggggtgggt tcaagttcca tggacgcgtc aaggcgctgg ccgctggcct tggtgaaaaa
     3001 ggagtgagga tctagtccgt cggagcgcat gcctgaccgt atagctatat cgcacgctac
     3061 taaggtcacc tcgatgatgt tatcagttgg tcccctcgac gagttgccgg catagtggaa
     3121 gagaaggaga agatttgaag cagattaaag ctgacgcgct taacctggcc gagcgtatcg
     3181 ttcatatcaa ccgggtcgct aaggtggtta agggtgggcg gcgatttcac tttagtgccc
     3241 ttgtcgtggt aggcgatcaa aatggacacg tcggaatagg attgggaaaa gcgcacgagg
     3301 taccggaggc gattcgcaag gggatcgagg ctgccaagaa ggaattgatt ggcatacaac
     3361 tgcagcgaac gagtattccc catgaggttg tcggaaggta cggcgcagcc aaggtgatct
     3421 tgaggccggc ctcacaagga actggggtcg tggcgggaaa ggcggcccgc gcagttctgg
     3481 aggcggttgg tgttcgggat gtcctgtcta aatcgctggg atcagacaat ccccacaatg
     3541 ttgtgaaggc aacattgaag ggcttacagt tacttcggtc gcctgaggaa gtcgctcgcg
     3601 ccagaggggt gcgccaggaa ggactggtcg cgcaaggggc ataagatgga caaaggtctt
     3661 cgcatcattc tccgaaaaag taagatcggc cacccggcac atcaggcccg ggttctcgcg
     3721 ggattagggt tgcgcaagat caatggatct gtggttcgcg ccgacacccc tactattcga
     3781 gggatgatca ggaaggtggt ccacctggtc gatgtacagc aggttgatag cgaggagaag
     3841 ggatgaagct gcacgagcta aagccgccag ccggcgccag tcaacgcaga aaacgcgttg
     3901 gacgcggtac cggctcgggg catggaaaga catccggtag aggagaaaaa ggtcagaagg
     3961 ctcgctctgg tgcccatatc catccctggt tcgagggcgg tcagcttccg ctacaccgcc
     4021 gcgtgccaaa gcggggattt actaacaagt ttagaaaagt ctacgctacc gtgaacctga
     4081 aagatctgga gcgattcgaa gcaggcacca cagtgacccc aggactgctt caggagaggc
     4141 ggattgtcaa ggatctgaag gtggggttga aggtgcttgc cgaaggggtg cttagcaagc
     4201 ctctgaacgt agctgcgcat aagttctcca agcgatcaat tgagaagatt ctcgcctccg
     4261 gtgggatggt cgaggttatt agtgcatgac tgattgggct ggcaacatct tcagggttcc
     4321 tgaacttaag aagcggatta tctttaccgc tctcgcgctg atcgcgtacc ggctagggtc
     4381 ccacatcccg acgccaggga tcgatgctca tgccctttta tcgttttttc agcaggcagg
     4441 cgggacgctt cttggcttct ttgatctctt ttcgggtggc gcactgcgtc gcctcagtat
     4501 tctcgcgctc ggtatcatgc cgtatattag cgcgtctatt atccttcagc ttcttgcagt
     4561 cgttttcccc cccttagaga agctgagtaa agagggggag gctgggcgca agaagatctc
     4621 tcaatatacg cggtacggta ccgtcctgct tgcggctatc caggccatgg gaatcgccat
     4681 cggccttgag aacatgcgga gcccgactgg ggagcagatc gtggtggatc cgggagtagt
     4741 ctttcgcctg atgaccacga ttacgctgac gacagggtcg atcttcctca tgtggcttgg
     4801 cgagcagatt accgagcgag gaatcgggaa cggaatctct ctgctgatct ttgccggtat
     4861 cattgtccgg gtgcctgagg cgatcgtcaa tacttggcgg cttctgacca cgggggagtt
     4921 gcaggctctc gtgctgctga tcgtcctggc cctgatggtg ttggttgtgg ccggagtaat
     4981 catcatgacg ctcggccaaa gaccgatagt agtacagtat gctaaaagga tcgtcgggcg
     5041 taagatctat ggaggccaga gtacgcatat tccgttgagg atcaatacgg ccggtgtcat
     5101 tcccgtaatc tttgcttcct ctatcatcgt gtttcccgct acgatcgcac aatttattaa
     5161 tcatccctgg atgcagggtg tggcccgggc gctttctccg ggtactgtga cctatacggc
     5221 tctgtatgcg gtcgcgatca ttttctttac atatttttat acggccatcg tcttcaaccc
     5281 gactgacgtt gctgataaca tgagaaagca tgggggcttc atcccgggca ttcggccagg
     5341 atcgaggacg gcggaattta tcgagaaggt gttggatcgg attacacttg ttggggcgat
     5401 ctacctgacg ctgatatcaa ttcttccgga attgatgatc accacgatga gcgttccgtt
     5461 cttctttggg gggacatccc tcttgattgt ggttggcgtg gccctggata ccgtacagca
     5521 agtggagtct cacttgctca tgcgtaacta tgagggattt ctaaagaagg gtcgagttaa
     5581 aggcaggctg gggtagttga gctatgcggc ttgtcttgct gggaccgcct ggcgctggta
     5641 aggggacaca ggccaggctg ttgacggcta agtctgatgt cgtgcatgtt tctgctggtg
     5701 atctgctgcg gcaagctgtg gctgacggct ccgagctggg gcaaaaagcc aagtcgatca
     5761 tggcgcaagg gtcccttgtt ccagatggag tggtcaccgg gattatcgaa gagcggttgc
     5821 gcaggcccga ttgtgtcagt gggtacatcc ttgatgggtt cccaaggaca cttcgtcagg
     5881 cagaggcgct ctcggatgtt ctgaggcttt tgcaggcccc gttagattgg gttatcagcg
     5941 tagaggtgtc cgaggacgac ctggtcagac ggcttgccgg gcgacggatc tgccgggcct
     6001 gtggggctat gtttcacgtt gacacgaaac cgccaacgca acatgggatc tgtgatagct
     6061 gcggggacca actcttccag agggacgacg ataaggagga tacgatccgc catcgccttc
     6121 gagtctaccg ggagcaaaca gagcctctga tcgcatatta ccatacgatg gggctgctga
     6181 ggcggatcaa tggtcagggg acgatcgagg agattgctca acgcatccat caggccttag
     6241 gggacgggta agcataatta tgatggtctt gaagtcccca tgggagatcg atttgatgcg
     6301 aaagagcagt cggctcgtgg cggaaactct cgggaggttg acgagactta tagagccggg
     6361 gctaacgacg ttggagttag atcgcgctgc agaggcttat atcttgaggc gagggggtaa
     6421 gccggcgttc aagggctacc gaggctaccc ttatacgctc tgtgcgtccg tgaacgagca
     6481 ggtggtccac gcgttcccgt cggcgaggaa gcttgaggag ggtgacatcg taagcctgga
     6541 tctcggagtg attgtcgatg gctactacgg ggatgctgcg attacggttc ctgtagggaa
     6601 ggtatcggaa ggggccaggc gtttgattgc cgcaactcga gaggccctta cgcgggctac
     6661 catggcggca ctgccaggga atcatctctc cgacatatct catgctgtgc agtccgtcgt
     6721 tgaggctaga ggattctccg tagtgcgcct gtttgttggt cacgggattg gccggtccct
     6781 tcatgaggaa ccacagatac cgaatttcgg gccaccagcc caaggcccca tgctcaaacc
     6841 tgggttagtc ctggccatcg agccgatggc caatgccgga ggcccggacg tgatgatcct
     6901 cgatgatcac tggacagcgg tgacatgcga cagttccctt tccgctcatt ttgagcacac
     6961 cgttgcgctg accgaggatg gggtagaggt gctgagctgc ctgaccgagg atgagtcacc
     7021 tcaaggaggg ggccgctagt gccaaaggaa gaggcggttg aggttgaggg gacggtggtt
     7081 gagcctttac ctaacgcgat gtttcgtgta gaactggaaa ccggacacaa ggtccttgcg
     7141 cacatctctg gaaaaatgcg catgcatttc attcggattc ttcctggaga taaggtgatt
     7201 gtggagttgt ctccatacga tttgacgcga gggcggatca cgtaccgata taaataggca
     7261 tcacgaagga tggtcaatgg cgatcagtga tgctgtgatg cgggggctac tatgaaggtt
     7321 cgggcgtcag ttaagccgat gtgcgagaag tgtaaggtca ttcgacgtaa aagggtgctg
     7381 cgggtcgtgt gcgacaatcc tcggcataaa cagcgacagg ggtagatggg ttcacagttc
     7441 agagtttatg ccagagatgg tgtcatgtat ttgagcgccg ttctatgaac tatgcactct
     7501 gaactaagaa agggagttgt atggcacgca tcgccggggt agatcttccg agggaaaaac
     7561 gtcttgagat tgcgctgacg tatattttcg gcgtcggtcg tgctgcttcg cataagatct
     7621 tgcaggactc cggagtcacc ccggatgtcc gcgttaagaa cctcactgaa gaggagatcg
     7681 cgaagctgcg gcgaaccatt gaaggaaact acagggtgga aggcgacctg agacgtgaga
     7741 tctctctgaa catcaagcgg ctcatggaca ttggcgccta ccgtggtctt aggcatcgcc
     7801 ggggtcttcc tgttcgtggc cagcgaacca gcaccaatgc caggacgcgc aaggggcctc
     7861 gccggacggt cggcatgaaa agtaagaaag tgtagtcgga taggttcata gttcagagtt
     7921 catggtgggt tgtcaaacac aggccacgat ctctgatctc taaacgatac gctatgaact
     7981 gtgaaccatg aacactcgct tgatggagga agaatggcgg aggaggtaaa accgagacgt
     8041 ccggccggac gtacgggcgg caggaaagag accaagaaca tcgctcacgg aatcgcctgt
     8101 gtccaggcga cattcaacaa tactattgtc accatcacag acacgacagg caatgttgtt
     8161 gcatgggcta gtgctggctc tgtggggttc aaaggttccc gaaagagcac cccgtttgct
     8221 gcgcagaggg cggcggatag tgctgcgcag agggcgatga gccaaggcat gaaagaagtg
     8281 aaggtgtata tcaaaggacc aggcgccggt cgagaagctg ccattcgggc cctacaggcg
     8341 gcaggcatgg agatctcgac gattaaggac gtgacgccga ttccccacaa cggttgtcga
     8401 gcgtccaagc ggagacgagt atgatacagt tgttctggcc gatatatcgt ccagtcaccc
     8461 tcagaaggga ggccaggatc gtaatccgcg gaagtgtcag tgagacgggg ggtggcacta
     8521 ccccctgggg aggaggggtt cgtggctagg tatctggatt ctgcgtgcaa gctttgcagg
     8581 cgggaagggc tgaagctgtt cctgaaaggg gatcgatgcc tctctaccaa atgtgccatt
     8641 gagaagcgga acttcattcc aggcgtgcat ggccaacgac gaacaaaggt ttcggattac
     8701 tgcaggcagc ttcgcgagaa gcagaagatg cgccgaatct acggggtgct ggagacgcag
     8761 ttccggatat actatcgtct ggctgagcga caaacaggga tcacaggcga aaaccttgtc
     8821 aagattttgg agcaacggct cgatagcgtt gttcatcgtc tcggatttgc cgcatcccgc
     8881 gcacaagcga ggatattgat tacccacggt cacatcctcg tcaatggacg aaagaccgac
     8941 attgcgtcct atcgggttcg tccaggagag accatcgagg tgcggccaaa gagtcgcgaa
     9001 atagcggcaa ttaagacggc cttcgaagtg gcaaagcggc gaacgttgcc gagctggcta
     9061 gagcttgatc cg
//
LOCUS       DATMXI010000297         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_98693, whole genome shotgun sequence.
ACCESSION   DATMXI010000297 DATMXI010000000
VERSION     DATMXI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98693"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..597
                     /locus_tag="VN444_05685"
     CDS             283..597
                     /locus_tag="VN444_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21336.1"
                     /translation="MKVTRYCYLLTMHDRKRHEHTTVEGKLTRFVVQDETFVEGEWHP
                     VVRYDTAHGFPHVDKMYADGTRAKIPLLTMDMGEALTVADEDIEENWERYKAAYSKGK
                     ER"
     gene            594..863
                     /locus_tag="VN444_05690"
     CDS             594..863
                     /locus_tag="VN444_05690"
                     /inference="COORDINATES: protein motif:HMM:NF039814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5647 family protein"
                     /protein_id="HWR21337.1"
                     /translation="MTEHELFVKNLRLSHEFDLYLTEHPEVAEQIPENVLIVLLPEED
                     PELCAKNRELAMARRQPGQPLVYVRVEKLTPPRSRLVNPRVEVAA"
     gene            1198..1584
                     /locus_tag="VN444_05695"
     CDS             1198..1584
                     /locus_tag="VN444_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWR21338.1"
                     /translation="MPTALELTREGWKPYLEAARRRPAPPEPTPAERRERARLLARVR
                     EAAEALKARFEVRRVVLFGSLAHEAWFVPDSDVDLAVEGLAGSDYWRAWRLVEEVIGD
                     RLVDLIEIETAGKSLREAIERYGVEL"
     gene            1605..2084
                     /locus_tag="VN444_05700"
     CDS             1605..2084
                     /locus_tag="VN444_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HWR21339.1"
                     /translation="MERIRGEIPDLDRVVQRASAAWAQAKRMPGESAYLDSVALNLHA
                     FYSGLERLFELIARHVDGALPSGEAWHRDLLQQMAQDMARVRPAVLRQDSALTLDEFR
                     RFRHLVRSVYTMSLLPEKMTGLMAALSTLWPALRAELLAFADFLEVLGQAGQSEEQR"
     gene            2245..2661
                     /locus_tag="VN444_05705"
     CDS             2245..2661
                     /locus_tag="VN444_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21340.1"
                     /translation="MKKKKWHSYGVADIVRDAQRGIPSGLDSFCGKAEVQKEMRKKQK
                     ISTEVLRALNYSSPEEAALDMIFLSARSRYSEFSQEVRQFEEKYHMDIETFQRTADVR
                     VREENFGQEEDLMAWKFAKEAAEYWRQKIEELERAA"
     gene            2651..3043
                     /locus_tag="VN444_05710"
     CDS             2651..3043
                     /locus_tag="VN444_05710"
                     /inference="COORDINATES: protein motif:HMM:NF039803.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HWR21341.1"
                     /translation="MLLDLAKQYADIVQGVEVEKFRIVGDSYELRATFLLKDGSRLFV
                     KDYLFLDGTRKYAYHWQDRPGRLINRWDNVPHWNELQSYPHHRHVGAADVVEASDVRT
                     IEDALKYIANSFRPGSRRLSQAAKRRGT"
BASE COUNT          738 a          792 c          913 g          620 t
ORIGIN      
        1 gtcgcaacgt cacttcacct ggacgccatg aacgctatcg acgccatcgt ctcgcctttc
       61 ccgctcgtcc cgcttctctc gccgcgctga cgccataaac gcaataaacg ccgcacgcaa
      121 tcgactcaaa aggagctgca ttctggacac ggtccctcct ttagcctata gtgactacgg
      181 accctttcag cgctggaggg gttccgttag tgcattgcgg aggtctagac tggttcccgc
      241 actgaaggcg gaagagccag atgtggtgaa ggatgccacc tcgtgaaggt aacccggtac
      301 tgctacctgc tgacaatgca cgacaggaag cggcacgaac acacaacagt tgagggaaag
      361 ctcactcggt ttgtcgtgca agatgaaacc ttcgtggagg gagagtggca cccagtcgtc
      421 cgatacgata cggcccatgg attcccccac gtggataaga tgtacgcgga tggtacaaga
      481 gcaaagatcc cgctgcttac aatggatatg ggcgaagcgc tgacggtggc tgatgaggac
      541 atcgaggaaa actgggaacg gtataaggct gcgtactcga aagggaaaga gagatgacgg
      601 aacacgagtt gtttgtcaag aacctcagat tgtcccatga attcgatctc tatctaacgg
      661 aacatccgga ggtggcggag cagattccgg agaacgttct gatcgtgctc cttccagaag
      721 aggacccgga attgtgtgcc aagaacaggg aactggcgat ggcccggcga cagcctggcc
      781 aacccctggt ctatgtccgg gtcgagaaac tcaccccacc ccgttctcgg ctagtgaacc
      841 cccgcgttga ggtagcagcg tgagccaagc cggccggctc taccaggact ccatcgactc
      901 gccacacgcc atcgacgcaa tagcctcgct catctcgctt gtcccgcaga ccaaatagac
      961 caaatggacc agacagacca aacgaccagg taaacgccat gaacgccgca cgcaacgaac
     1021 gcctttgtcc tgtagcggcg tatttacttt actcagatga ctcccggggt taacgtagtc
     1081 gtgtggacga agactacccg aaccccttag cacgtgaggt caaagggttc gctcctcatt
     1141 ccttgctgag gttcgcatgg acaatatcgc aggatgggca cgagaggaga agggggtatg
     1201 ccgacagctt tggaattgac ccgagaggga tggaagccgt atctcgaggc cgcccggcgc
     1261 cgtccggctc caccggagcc aactccggcg gagcgccgcg aacgtgcacg tctgctggcc
     1321 cgcgttcggg aggctgcgga ggcgttaaag gcccgcttcg aggtccgacg ggtggtgctc
     1381 tttggctcgc tggctcatga agcctggttt gtgccggact ccgatgtgga cctggctgtt
     1441 gaaggtctgg caggctctga ttactggcgg gcctggagac tggtagaaga ggtgattggt
     1501 gatcgcctgg tggatttgat cgagatcgag acggcgggga aatcgctgcg tgaggcgatt
     1561 gagcggtatg gagtagaact ctgaacgcgc tctaccagga attggtggag cgaatccgtg
     1621 gtgagatccc tgatctcgac cgtgtggttc aacgggcctc agccgcctgg gcccaggcga
     1681 aacggatgcc gggcgagagc gcgtacctgg attccgtagc cttgaacttg catgcctttt
     1741 attcgggatt ggagcgcctg tttgaactaa tcgccaggca tgtagacggc gcgctcccca
     1801 gtggcgaagc ctggcatcga gacttactcc agcagatggc acaggacatg gcccgcgtgc
     1861 ggcctgccgt gctcagacag gacagcgcac tgactttgga tgaatttcgg cggttccgcc
     1921 acctggtgcg cagcgtgtat accatgagcc tgctgccgga gaaaatgacc gggctgatgg
     1981 cagccttgtc caccctgtgg cctgcgctgc gtgctgagtt gctggccttt gcggactttc
     2041 tggaagtgct cgggcaggca ggccaatccg aagaacagag ataaacaaag gagaaaagcc
     2101 gtacgcaata aacgcggcac gcaatcaacg ccatcgtctc gcctttcccg ctcgtctcgc
     2161 tctcgcaaca cgcgcaatcg acgcaataga cgccgcacgc aatgacgcaa tgacgccatc
     2221 gacgcgataa actcataggt agccatgaag aagaaaaaat ggcatagtta cggtgtggct
     2281 gacatcgtaa gagatgctca gaggggcata ccctcagggc tcgattcttt ctgcggaaag
     2341 gcggaggttc agaaagagat gcggaaaaaa caaaaaatat cgactgaagt tctaagggct
     2401 ttaaattatt caagtccaga ggaagcggca ctggacatga tcttcttatc tgctcggtcg
     2461 cgatactcgg agttcagtca agaagtcaga caatttgaag agaagtatca tatggatatt
     2521 gaaacgttcc aaaggacagc cgacgtacgg gtaagggaag aaaatttcgg acaggaggag
     2581 gatctgatgg cgtggaagtt cgccaaagag gcagccgagt actggcggca gaagattgag
     2641 gagttggagc gtgctgcttg acctggcgaa gcagtatgct gatattgttc agggtgttga
     2701 ggttgagaaa tttcgcattg ttggagacag ctacgaatta agggcgacct tcttactcaa
     2761 ggacgggtca aggttgttcg taaaagatta cctttttctg gatggtacca ggaaatacgc
     2821 ctaccattgg caagataggc ctgggcgcct gatcaaccgg tgggataatg ttccccattg
     2881 gaatgagtta caatcgtatc cacatcatcg gcatgtgggg gcagcagacg tcgttgaggc
     2941 ctctgatgta cgaacgatag aggatgctct gaaatacatt gccaactctt tcaggccagg
     3001 tagcagacgc ctatcccagg ctgctaagag acgtggaaca taaatcgcgc ctactctcca
     3061 atc
//
LOCUS       DATMXI010000298         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_309030, whole genome shotgun sequence.
ACCESSION   DATMXI010000298 DATMXI010000000
VERSION     DATMXI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309030"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="lpxD"
                     /locus_tag="VN444_05715"
     CDS             complement(<1..972)
                     /gene="lpxD"
                     /locus_tag="VN444_05715"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWR21342.1"
                     /translation="MQLRELAEKLRCRLVGNGEVEVYRLAPLHEAGEGDLAFVVNARD
                     LPTLEVTRATAVIVREGSPPCSKPALLTNDPYLAFVQALRLFYTPDRPTPGVHSSSIV
                     QEGVRLAEDVAIGPLSVVEAGVTIGQRSVVGAQVYIGEGSRIGVDCRLYPQVMIRDGV
                     EIGDRVIIHSGAVIGSDGFGYLRDGQGVRIKIPQVGRVILEDDVEIGANVTIDRATMG
                     ATRIKRGTKIDNLVQIAHNVVVGADTVIVALSGISGSTKIGDRVTLASQVGIVDHVEI
                     GDDVTVGAQAGVSKSLPPGGVFLGSPAVPHLAFKRSVAAVNRLPQILN"
     gene            complement(981..1529)
                     /locus_tag="VN444_05720"
     CDS             complement(981..1529)
                     /locus_tag="VN444_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWR21343.1"
                     /translation="MARSNRGGRWWLFLGATAVVLMASTSWAADPPMRLGFVDFQMVI
                     SQSKEGQAAMATVKAEATEKQKDISAKEAEIKQMDADLQKQASALSDAAKKDREEEIR
                     RKLRDLKRVTEDFNRDLAKRESEMVNDLLRDLTVVVRDYGKEKGFTLIVEKGQSGVIY
                     SNDAADLTKEILERYNTRRSKK"
     gene            complement(1549..>2713)
                     /gene="bamA"
                     /locus_tag="VN444_05725"
     CDS             complement(1549..>2713)
                     /gene="bamA"
                     /locus_tag="VN444_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWR21344.1"
                     /translation="IRREIPLIEGDLYNSRLLARGRQNLNNLGYFEDVKVETRRGTSE
                     DRVDVDVVVKEKPTGSFSIGGGFSSIDGMMGSGSITQDNFLGLGQRVSLSAQLGVRAT
                     RFVMNFFDPHILDTETSLDLSLFNQRLLFNEQVGYNQDIKGGSIALGRRLYKQLFGTL
                     GYRYEVNRIFDVNENAPDEIKDQKGTNMTGRVSTGLSLDLRDNPRDPTRGFSGAVTYQ
                     IAGTFLGGENKFQRLNLDLGYYHPLIWKLVGHVRGNLIVAEPYGKKDLPVQELIYLGG
                     TNTVRGFKTFHLSPIDPATGERIGGNKAIYFNNEVMFPIYEPLGLRGLLFFDAGNVWT
                     KKESFSLDLRPTAGGGVRVATPFGLVRVELGLNLDKRPGESSSAMHLTVGSTF"
BASE COUNT          583 a          876 c          672 g          582 t
ORIGIN      
        1 attcaggatt tgggggagcc gattcaccgc ggcgacgctg cgcttgaatg cgagatgcgg
       61 tacggcggga gaacccagga agacaccccc aggcggaagg ctcttactca ctccagcctg
      121 cgctcccacg gtaacatcgt cgccgatctc gacatgatcg acgatcccga cctggctggc
      181 cagcgtcacc cgatctccga tcttcgtact tccggagatg ccgctcaggg ccacaatgac
      241 cgtatctgct ccaacgacaa cattatgtgc aatctggacc agattatcga tcttggtccc
      301 acgcttgatc cgcgtcgccc ccattgtcgc tcggtcgatt gtcacgttgg caccgatctc
      361 cacatcatcc tcaagaatca cccgccccac ctgcgggatc ttgatacgga ccccctgtcc
      421 gtccctcaga tacccgaagc catcgctacc gatcacagcg ccgctgtgga taattactcg
      481 atcgccaatc tcaacgccgt cccgaatcat gacctgaggg tagagccggc agtcaacacc
      541 gatgcggctc ccctcgccga tgtagacctg ggcacctaca acgcttctct ggccaatcgt
      601 cacaccagcc tcgacgaccg agagcggacc gatcgcaaca tcctccgcaa gacgtacgcc
      661 ctcctgcacg atactggatg aatggacgcc aggcgttgga cggtccgggg tgtaaaaaag
      721 gcggagggcc tggacaaaag ccagatacgg atcgttcgtc agtagcgccg gcttagaaca
      781 gggcgggctc ccttcgcgta caatcacagc agtggccctg gtcacctcca gcgttggcag
      841 gtcgcgggcg ttcaccacaa aggccaggtc tccctcaccc gcctcgtgca gtggagcgag
      901 acgatagacc tcgacctcac cgttgcctac cagccggcac ctcagctttt cagccagctc
      961 cctcagttgc atcaccacaa ctacttttta cttcggcggg tattgtagcg ttccaggatt
     1021 tctttggtga ggtcggccgc atcgttgcta tagatcacac cgctctgtcc cttctcaacg
     1081 atgagggtga accctttctc tttgccatag tcccggacca caacggtaag atccctgagc
     1141 agatcgttca ccatctcgct ttcccgcttg gccagatcac gattaaaatc ctcggtcact
     1201 cgcttgagat cacgaagctt gcgtcgaatc tcttcctctc tatccttctt tgcagcatca
     1261 ctcagggcgg atgcctgctt ctggagatcc gcatccatct gcttgatctc agcctctttc
     1321 gcgctgatat ctttctgttt ctcggttgcc tccgccttca ccgtagccat cgccgcctgc
     1381 ccctctttcg actgggagat gaccatctga aaatctacaa acccgagcct catcggggga
     1441 tccgcagccc agctcgtgga tgccatcaat accaccgccg tagcccccaa gaacagccac
     1501 catcgcccac cacgattcga tcgcgccata cgttgtttcc tcctagtgct aaaacgttga
     1561 tccgaccgtc aagtgcatgg cactgctcga ctcccctggc cgcttgtcca ggttgaggcc
     1621 caactcaacc cgcaccagac caaagggggt tgcaacgcga actccgcctc cggcagtcgg
     1681 tcgcagatcc aacgagaaac tctccttctt ggtccagaca ttgcccgcat cgaaaaagag
     1741 taagcccctc aggcccaatg gctcatagat tggaaacatc acctcgttgt tgaagtagat
     1801 ggccttatta ccgccgatcc gctcccctgt tgcaggatcg atagggctca gatggaaggt
     1861 cttgaacccc cgaacggtgt tggtaccgcc gaggtagatc agttcctgga ccggcaggtc
     1921 cttcttgccg tagggctccg ccacaatgag gttgccccga acatgaccga ccagcttcca
     1981 gatcagtggg tggtagtaac ccagatcgag gttgaggcgc tgaaacttgt tttccccacc
     2041 cagaaaggtc ccggcaatct gatatgttac agccccgctg aagcctctgg tcggatcacg
     2101 ggggttgtcc ctgaggtcta acgagagacc agtagagacc cgcccagtca tatttgttcc
     2161 tttctggtcc ttgatctcat ccggggcgtt ctcgttcaca tcaaagatgc ggttcacctc
     2221 ataccggtac ccaagggtac cgaacaattg cttatagagc ctccggccaa gggcgatcga
     2281 gccgcccttg atatcctgat tatagccaac ctgctcgttg aagagcagac gctgattgaa
     2341 cagcgagagg tcaagcgatg tctcagtgtc cagaatgtgg ggatcaaaga agttcatgac
     2401 aaaccgagta gccctgacgc caagctgcgc tgaaagcgag acccgttgtc ccaatccgag
     2461 aaagttatcc tgggtgatgg agccagagcc catcatccca tcaatcgaac tgaagccgcc
     2521 tccgatacta aaggagccgg taggcttctc cttcaccacc acatccacgt ctacccgatc
     2581 ctcggacgtt ccacgacggg tctcgacttt gacatcctcg aagtagccaa ggttgttcag
     2641 attctggcga ccgcgcgcca gcagacggct attatacaag tcgccctcga tcagagggat
     2701 ctcccttcgg atc
//
LOCUS       DATMXI010000299         5761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_224946, whole genome shotgun sequence.
ACCESSION   DATMXI010000299 DATMXI010000000
VERSION     DATMXI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224946"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VN444_05730"
     CDS             <1..1774
                     /locus_tag="VN444_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWR21345.1"
                     /translation="LEEVLAGGSEASSIEHHLRLLEEEIATAPPGVVDELLSHYGALQ
                     TKYEQLGGYSVEARAKEILFGLGFKEKHLSRPLENFSGGQRMRASLAQILLSSPDALL
                     LDEPTNHLDLESVVWLEKFLSGYAGAILLISHDRNFMNRLANRVVEVERQQLIAYTGN
                     YDRFVVAKAEAQQILEATVKNQQKKIEDTQAFIDRFRSKATKARQVQSRIKLLEKMDK
                     AELTPQQKRVRFSFPEPPRSGETVIRLIDIDKSYDNNPVYRALNVELRRGERVALTGP
                     NGAGKSTLLKLLAGVLPFERGERTLGHNVTLAYYAQHQLELLNPASTVLDEITLAAPM
                     EESSFLRAILGRFLFSGDDVKKKVAVLSGGEKSRLALAKMLIRPANLLLLDEPTNHLD
                     IPSRDVLEEALRDFTGTICFITHDRHFIRAVANRIIDVRDGEATPYAGDYDYYLYKKQ
                     LIAEEAAGSGQAGPGSTDLDAGGTLRSSPDTPVSLAQGPGVKEGRSKERKTKEQKRAE
                     AEARNRTHRTSRPLQSSLSTIEEEVAEAEKILQDLTQALADPEIYQNKARCLEALDAH
                     AQAKQRVADLTAEWDRISSLMVTT"
     gene            2056..2694
                     /locus_tag="VN444_05735"
     CDS             2056..2694
                     /locus_tag="VN444_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21346.1"
                     /translation="MSDARPSLSTHTTGACPACGARRVPGASACWQCGGTFATTPPTA
                     PASSLRWVTLPWVGVIPFGLFFLPWVIAGPYGVHRVLSVAGLELALGRTLRGRLVPHE
                     PLLWLIPIASLLLAALLTWARRRTPGPRYWLLVATIAWGAFLLFLIKAVQWLWVSPSP
                     GTPWVVHWLTTEYLVAISAFFVSGLSALRVWMGARIPRPQSSSSSPSEDTPW"
     gene            2694..3503
                     /locus_tag="VN444_05740"
     CDS             2694..3503
                     /locus_tag="VN444_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWR21347.1"
                     /translation="MEGQFDSASQGDTADPFAGQTKVGTCGFAIGRQEYYTTFPVVEI
                     QQTFYKLPRVSTGVRWRAGAPIGYEFTMKAWQLITHEPSSPTYRRLAKPIPLELKACY
                     GAFRPTEEVLEAWAQTRAFAAALGATIIVFQCPPSFTPTSEHIANLRHFFTTIDRTGW
                     QAAWELRSAWAADMILGLCRELDLIHAVDPLKEPSLHGAICYYRLHGLTGYRYVHTDQ
                     DLERLKTVCKGDLPTYCLFNNLFMAEDAVRFQALLGEKAKPLRARRGRAEP"
     gene            3484..4647
                     /gene="queG"
                     /locus_tag="VN444_05745"
     CDS             3484..4647
                     /gene="queG"
                     /locus_tag="VN444_05745"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWR21348.1"
                     /translation="MAVQSPEQLAQAIKDEAGRLGFKLVGISPVSDPPHEQSFADWLQ
                     GGYSGEMAYLTRTEQARRHPETWLPWARSVVSVAMNYYTPFLRETHQTGVPRGWISRY
                     AWGEDYHTILESRLDALFGWIRHTVGEEVQGKACVDAGPVLEKGFAGLAGIGWIGKNT
                     LLVSPKHGSYFFLGELFLSLELPADEPIRDRCGSCDLCLKACPTDAFVGPYRLDARRC
                     ISYLTIELKGSIPAQMRPLIGNHVFGCDICQEVCPYNVNVEASKEPVFQPREGLHAPG
                     LIPLLALNEEQFRSQFKGSPIRRPKRRGVLRNVAVALGNLGSTESVPALAKLLSDPES
                     LVRGHVAWALGQIGTTEAHAALNEAFDRETDPDVKAEIEQALTACALSPSPGP"
     gene            complement(4610..5320)
                     /locus_tag="VN444_05750"
     CDS             complement(4610..5320)
                     /locus_tag="VN444_05750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR21349.1"
                     /translation="MVEWKRQRVERTYRPGERHEAKHGSLSDTLLRLIREESLRERTV
                     LDVGCGKGRLTFALAEEAGRIIGIDWSDQLIQEAGQHARTLGFDHITFMCGDAERIEY
                     RELGPIDLVVAHLCMSDEIIRRAAAVLAPGCCIAFSTLHQDQWKESGRSSRFAYGEQD
                     VETALTAAGFDPVYLEAEHEVLSFAAAADALAYMEALDLIGKWKTDSRWEGFLAYLER
                     GGRELTIRARVTVKARRR"
     gene            5495..>5761
                     /locus_tag="VN444_05755"
     CDS             5495..>5761
                     /locus_tag="VN444_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWR21350.1"
                     /translation="MRQQLSNFKAEFFKALAHPLRISILDALRTGELTVNEISRQFGV
                     EPANASQQLGVLRNKGIVVARKEGSNVYYSVSDPAIFTLLDAARE"
BASE COUNT         1151 a         1742 c         1739 g         1129 t
ORIGIN      
        1 gcttgaagag gttctggccg gcggctcgga ggcgtcgtct atcgagcacc atctccgcct
       61 tctggaagag gagatcgcca cggccccacc gggggtggtg gacgagctgt tatcccatta
      121 cggtgcgctc cagaccaagt acgaacagct cggcggatat tccgtggagg ccagggcaaa
      181 ggagatcctc ttcggcctcg gtttcaagga gaagcacctt tccaggcctc tggagaattt
      241 ctccgggggc cagcggatgc gcgcctcttt ggcccagatc ctcctctcct ctccggacgc
      301 gctcctgttg gacgaaccca cgaaccatct ggatctggag tcggtcgtct ggctggagaa
      361 gttcctgtcc ggatatgccg gcgcgatcct gctcatctcc cacgatcgga atttcatgaa
      421 ccgtctggcc aaccgggtgg tggaagtgga acgacaacaa ctgatcgcct ataccggaaa
      481 ctacgaccgg ttcgtagtgg ccaaggccga agcccagcag atcttagaag ccaccgtgaa
      541 gaaccagcag aaaaagattg aggacaccca ggccttcatc gaccgtttcc ggtccaaggc
      601 caccaaggcc cgccaggtcc agagccggat caagctgctg gaaaagatgg acaaggcgga
      661 actgaccccc cagcagaaac gagtccggtt ctcatttccg gagcctccac ggagcggaga
      721 gacggtcatc cggctgatcg atatagacaa gtcctatgac aacaatccgg tctaccgcgc
      781 cctgaatgtt gagcttcgcc ggggggaacg ggtggccctg acaggcccaa acggggcggg
      841 gaaatccacc cttctcaaac ttctggccgg cgtcctgccg ttcgagaggg gcgaacggac
      901 actgggccac aatgtgacac tcgcctatta tgcccagcac cagttggaac tcttgaaccc
      961 cgccagtacg gtccttgacg aaattacatt ggccgccccc atggaagagt cgtccttcct
     1021 gcgggcaatt ctggggcggt ttctcttttc cggggatgac gtaaagaaga aggtcgccgt
     1081 cctatcaggc ggagagaaga gccgattggc gctagccaag atgttgatcc gtccggccaa
     1141 ccttcttctg ttggacgagc cgacgaacca cctggacatc ccctcccgcg acgtgctgga
     1201 agaggcgcta cgcgacttca ccggaacgat ctgcttcatc acccatgacc ggcattttat
     1261 ccgggccgtg gccaacagga tcatcgacgt tcgggatgga gaggccacac cgtatgccgg
     1321 ggattacgat tactacctct ataaaaagca actgatagcg gaagaggccg ccggtagcgg
     1381 ccaggccgga cccggttcca cggacctgga tgccgggggc acactccgct cttctcccga
     1441 cacccctgtc tccctggcac aaggacccgg ggtgaaggag ggacgttcga aggaacggaa
     1501 aacgaaggaa cagaaacggg ccgaggccga agcgcgcaac cggacgcacc ggacgtcacg
     1561 cccgctccaa tcgtccctgt ccacaatcga ggaggaggtg gccgaagccg agaagatcct
     1621 gcaggatctc actcaggctc tcgccgaccc ggagatatac cagaataagg cgcgatgcct
     1681 tgaagcgctg gatgcgcatg ctcaggccaa acaaagggtg gcggatctga cggcggaatg
     1741 ggatcgaata tccagtctga tggtaactac ttaggtggat agactgtagg cttttagact
     1801 gtaggaacgc gcacagaagg gccaggcgtt ttccgaatgc gacgcgaaaa tgatggagat
     1861 cgaaaaggtt ctgggtacct tgattcggtc catgcgcaac gactaacagc cttcagccta
     1921 cagcctaaac acctgagtag tgacctgctc ggatagacag gctgaaggct atgaaggcct
     1981 tgcacggatg gcaagacctt gactggcaga accctggcct tcgcagtata attatgtggg
     2041 gaggtagacc ttccgatgag tgatgcgcgg ccatcgttga gcactcacac gactggcgcc
     2101 tgtccggcct gtggtgcgcg tagagtgcct ggagccagcg cgtgctggca gtgcggcggg
     2161 acgttcgcta ctaccccacc aaccgcacca gcttcttctc tacgctgggt cacgttgccg
     2221 tgggtggggg tgatcccctt cgggcttttc ttcctgcctt gggtcatcgc cggcccatac
     2281 ggggtccatc gcgtcctctc ggttgcgggc ctggaactcg ccctcggccg gacactcaga
     2341 ggccgtcttg taccgcacga gccgcttctg tggctcatcc ccatcgcatc actcctgctg
     2401 gccgcactgc tgacctgggc ccggcggcgg acacctgggc cgcggtactg gctgctcgtg
     2461 gcaaccatcg cctggggggc ctttctgctg ttcctcatca aggcggtgca gtggctgtgg
     2521 gtcagcccgt cccctgggac gccctgggta gtacactggc tgaccaccga atacctggtc
     2581 gccatctcgg ctttcttcgt gtctggcctg tccgccttgc gagtgtggat gggcgcgagg
     2641 atcccccggc cgcaatcatc ctcttcttct ccgtccgaag acaccccatg gtgatggagg
     2701 gacaattcga tagcgcgagt cagggcgaca ctgccgatcc tttcgcgggc cagaccaagg
     2761 tcgggacctg tggcttcgcc attggaaggc aggagtacta cacgaccttc ccggttgttg
     2821 agatccagca gaccttttac aaacttccgc gggtcagcac aggggtgcga tggcgagccg
     2881 gggcgccgat cgggtacgag tttacgatga aggcatggca gctaatcacg cacgagcctt
     2941 caagcccgac ctatcgccgc ctcgccaagc cgatcccact ggagctgaaa gcgtgctacg
     3001 gggcattccg gcctacggag gaagtcctcg aagcctgggc ccagacgcgg gcatttgcgg
     3061 cagccctggg cgccacgatc atcgtcttcc agtgcccgcc gagtttcacg ccgacgtccg
     3121 aacacatcgc gaacctgcgc cacttcttca ccaccatcga ccgcaccggc tggcaagccg
     3181 cctgggagct acgcagcgcc tgggcggcgg atatgatcct ggggctctgt cgcgagttag
     3241 acctgatcca cgcggtcgat ccgctcaagg agccatcgct tcatggcgcg atctgctact
     3301 accggctgca cggtctcacc ggctaccggt acgtccacac cgatcaggac cttgagcgac
     3361 tgaagacggt gtgcaagggg gatctcccca cctactgcct gttcaataat ctctttatgg
     3421 ccgaggatgc ggttcgattc caggcgctgc tgggggagaa ggcgaaaccg ttacgagcgc
     3481 gacgtggccg tgcagagccc tgagcagttg gctcaggcga tcaaagatga ggccggccgt
     3541 ctggggttca agctggtcgg gatctcgccg gtcagcgatc ctccccatga acaatcattc
     3601 gccgactggc tacaaggggg gtacagtggc gagatggcct atctgactcg cactgagcag
     3661 gcccgacgcc acccggagac gtggcttccc tgggcgcgct cggtggtctc tgtcgctatg
     3721 aactactaca cccccttcct acgcgagaca caccagacag gtgtgcccag aggatggatt
     3781 tcccgctatg cgtggggaga ggactatcac acgatcctgg agagccgact tgacgcgctg
     3841 ttcggctgga ttcgacacac cgtcggcgag gaggttcagg ggaaggcctg cgtcgatgcc
     3901 gggccggtat tggagaaggg gttcgccggc ttggcgggaa tcgggtggat cggcaagaat
     3961 acgctgctcg tctccccaaa gcacggctcg tatttcttcc ttggcgagct gttcctgagc
     4021 ctcgaactgc cggcagatga accgatcagg gaccggtgcg gctcatgcga tctatgcctc
     4081 aaagcctgtc caactgatgc ctttgtcggc ccatatcgat tggatgcccg tcgctgcatc
     4141 tcgtacctga cgatcgagct gaagggcagc atcccggcgc agatgcggcc actgatcggc
     4201 aatcacgttt ttggctgtga tatctgccag gaggtctgcc cgtataatgt caacgtcgag
     4261 gcctcgaagg agcctgtttt tcagcctcgt gagggccttc atgcgccggg gttgatccct
     4321 ctccttgcgc tcaacgaaga acagtttcgt tcgcagttca aggggagccc tatccggcgt
     4381 ccaaagcgac ggggtgttct acgaaacgtg gcagtggcgc ttggcaacct gggctccacg
     4441 gaatcggtcc cggccctggc gaagctcctc tccgatcctg aatcgctggt ccggggccat
     4501 gtcgcctggg ctctgggcca gatcggtaca actgaggcgc atgcagcgct caacgaggcg
     4561 tttgaccgtg agaccgatcc tgatgtgaaa gcggagattg agcaggcgct taccgcctgc
     4621 gcgctttcac cgtcacccgg gccctgatcg tcagctctct cccgccgcgc tccagatagg
     4681 cgagaaaccc ctcccagcgg ctatcggtct tccatttccc gatgagatct aaggcttcca
     4741 tgtaggccag ggcgtcagcc gcggcggcaa aggacagtac ctcgtgctcg gcttcgagat
     4801 agacggggtc gaatcccgct gctgtgagcg ccgtttcgac atcttgttct ccgtaggcga
     4861 atcgggaact cctgccgctc tccttccatt ggtcttggtg aagggtcgaa aaggcgatgc
     4921 agcagccagg agccaggact gcggccgctc gtcgaatgat ttcgtcggac atgcagaggt
     4981 gggcgaccac cagatcgatc ggaccaagct cgcgatactc gatccgctcg gcgtcgccgc
     5041 acataaacgt gatatggtca aaaccaaggg tacgggcatg ctgccctgcc tcctggatga
     5101 gctgatccga ccagtcgatc ccgatgatcc ggccggcctc ttctgccagg gcaaaggtca
     5161 agcgcccctt accgcacccg acatccagca ctgtccgctc gcgtagcgac tcttcccgta
     5221 tgaggcgtaa gagcgtgtcg ctgagactac cgtgtttcgc ttcgtgcctt tcgccgggcc
     5281 gataggttct ctcgacccgc tgccgcttcc actcgaccat cgctcctcct tattccggtt
     5341 gcattgtata ccacgagttc agacagcgcg actctttcgg taaaagcggg agcgaagact
     5401 gacgttgatg cttgacgaga gtacacatag ggtatagcat atagtaatac ttgcagatgc
     5461 aatactacgc aatcgttcag atgttggagg ggcaatgagg caacaactga gcaacttcaa
     5521 ggcggaattc tttaaagcgc tggcccaccc gctgaggatc tcgatccttg atgcgttgcg
     5581 gacgggagag ctcacggtga acgagatcag ccggcaattt ggtgtcgaac ccgccaacgc
     5641 ctcgcaacaa ctcggagtgt tgcggaataa gggcatcgtg gtagcccgca aagaggggtc
     5701 gaatgtctac tattctgtga gcgacccggc catctttacg ttactcgacg cggccaggga
     5761 g
//
LOCUS       DATMXI010000300         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_309072, whole genome shotgun sequence.
ACCESSION   DATMXI010000300 DATMXI010000000
VERSION     DATMXI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309072"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            968..1447
                     /locus_tag="VN444_05760"
     CDS             968..1447
                     /locus_tag="VN444_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21351.1"
                     /translation="MNRSMSAAVLFAALIVSQPVWAHTDESLDAMPAPHGGQVRAAGP
                     YHLELVAKDGELVLHVTDHANNEINTTGAEGTANVQQGKAGSKTKVKLEPSQTNTLAG
                     SGEFQVTPETVIVVFVKMPEQEAYAARFTPLKPKSVGTGKQTGGDHDHGHGDQHKGH"
     gene            1565..2044
                     /locus_tag="VN444_05765"
     CDS             1565..2044
                     /locus_tag="VN444_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWR21352.1"
                     /translation="MSERETIRGFFEQDHDRLDELFKTFQQLKRSDFTKAEQAFKEFK
                     VGLQRHIVWEEDLLFPLWEKKTGMSDSGPTPAMRNEHRQIGQQLEAIHRKVETQNVDS
                     DQEEQGLLNLLGSHNRKEERALYPAIDQVASAEEREGVFLNLTNIPEERYKLCCGEH"
     gene            2115..2750
                     /locus_tag="VN444_05770"
     CDS             2115..2750
                     /locus_tag="VN444_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21353.1"
                     /translation="MSRIKELRASFDKKLDALEHQALAFEAQLTQTKDEAMQRLEQRK
                     QQLQDLLTQVQTDVQQSKEMAEQAKTEVRAKLEHLQVQLALGKADARDSFEEQRTKIL
                     KALTEFESVADQKLKGAAFESGRVWEDLVGRASTLEAELEALKGRFETEKTKQQVTLE
                     SKKQELLTELVSFKDRLKAKRASVQAKTDVFETDLRDGLDQIKTGFRRLFE"
BASE COUNT          694 a          635 c          802 g          621 t
ORIGIN      
        1 gtctctcttc tccttggagg gtgtgattcg aagttccctt gaggcttgga ggcttcttgt
       61 cattcgatcg gttgttcatg agaggggtgc ttcactctgt tctcacccaa ggagcctgtc
      121 ttgattgccg ggcgggggag accggttcca agattattta tcccacagca gagcggtgtc
      181 acaaaataca ttcgtaaatc caacaggttg agcctctggt ggataaggtg atgtagttgc
      241 gcagtccccc ttttatgcag atccattcga agattccatc cgagtagcgt cagcctatcg
      301 tggcgaacga tccgcgagca tctgcgacgt tctcggatcc cagaatccct aacaggttcc
      361 cacaaacaag cctccgattt ttccaggcct tgggtcacta ttctgtacgc acctcatttc
      421 cctcatgacg aacggttttt gtcgagccgc catacaagac aatggaggcg gcgtgaaact
      481 ctagaggcct gtcgggttgt tgccttttgc cactgcactc ttttatccta gggtctagaa
      541 tgcctcatgg tcatctttcc taccttcaaa cgcctcctca ggggcttgtt aattcacagg
      601 aattagagat tccctgggtc agcttgtctg gcacggcctt cgcttttcaa tggggcaagt
      661 cacgttactc cgaggaagaa tcgcgatgga caatcaccat ttggaatgtt cgttacaggc
      721 tggtgcagtc gcatactcag agggactacg gctggaaggg agaggtagtg gcaaggtcga
      781 gcaagagccc ttgaaagaga aagatgatac ctgtcgtaat tgagggcatg ttgtggttgt
      841 cactgtgagc gcatgggtcc ggctgaggat cgaggtcccg gcatccaccc tttgatgtga
      901 tagcggggac ttcaaaatgg cttagaacga tgtccggtca ttccgatatg tcatgtgagg
      961 agagaaaatg aatagatcga tgagtgccgc tgtgctgttt gccgctctga tagtatctca
     1021 gcctgtttgg gcgcataccg acgagtctct ggatgcgatg ccggcgccac atggtggaca
     1081 agtccgtgcg gcaggcccgt atcatttgga gcttgttgcc aaggatggag agctcgtact
     1141 gcatgtaact gatcatgcga ataatgagat aaacaccacc ggcgcggaag gtacggcaaa
     1201 cgttcaacag ggtaaagctg gcagcaagac aaaagtcaaa ctggaaccgt cacagaccaa
     1261 cacattggct ggtagcggcg agtttcaagt cactcctgaa accgtgatcg tggtatttgt
     1321 taaaatgccg gaacaggagg cctatgccgc tcggtttacc ccgctcaaac caaagtctgt
     1381 cgggacggga aagcaaacgg ggggagacca tgatcatggc catggcgatc agcataaagg
     1441 gcattgagct gtagaaggtt gcgtcgtcag gatcatgggc aaggatattg tttcttgagt
     1501 ctggatcata ggcgctcgtc tgtctgccta tagggttgcc gtcggcgtca atgaaggaga
     1561 catcatgagt gaaagggaaa caatcagggg gttttttgag caggatcatg accggttgga
     1621 cgagttgttc aagacctttc agcaattgaa gcggtctgac tttacgaagg ctgagcaagc
     1681 gttcaaagaa tttaaggtcg gcttgcagcg ccatatcgtg tgggaagagg atctgctctt
     1741 tcctctctgg gaaaagaaaa ccggcatgtc cgatagcggg ccgacgcccg ctatgcggaa
     1801 cgagcatcgc cagattgggc aacagcttga agccattcac cgcaaggtgg aaacccagaa
     1861 tgtggatagc gatcaagaag agcagggtct gttgaacctg ctcggctccc ataataggaa
     1921 ggaagagcgt gcgctctatc ccgccatcga tcaggttgcc agtgcggaag agcgagaagg
     1981 agtgttcctc aacttgacca atatcccaga agagcgttac aaactctgtt gtggagagca
     2041 ctgactagta tcgctaaaac acgcgatgtg gtgaccggtg tctatggtcc gtcttacaca
     2101 cagaggaggg tgttatgtcg agaattaagg aactcagagc ctcgttcgac aagaagttgg
     2161 atgccttgga gcaccaggca ttggcatttg aggcacaact gacccaaacg aaagatgagg
     2221 caatgcagcg actggaacag cgtaagcaac aacttcaaga tttgcttacg caggtccaga
     2281 ctgatgtgca gcagtcgaag gagatggcag agcaagcgaa gactgaagtg cgggccaagc
     2341 tcgaacatct tcaagtgcaa ttagctctcg gtaaagctga tgcgcgcgac tcgtttgagg
     2401 agcaaagaac gaagattctt aaagccctga ccgagtttga gtccgtagcg gaccagaaac
     2461 taaagggggc tgcgtttgaa tcgggcaggg tctgggagga tctggtcggg cgggcgagca
     2521 cgctcgaagc cgaacttgaa gcactcaagg gccggtttga aactgagaaa accaagcagc
     2581 aagtcacgct ggaatcaaag aaacaggagc tactcaccga attggtgtcg ttcaaggacc
     2641 ggctcaaggc caaacgcgca tccgtgcagg ccaagaccga tgtatttgag accgacttgc
     2701 gcgatggact ggaccagatt aagacggggt ttaggaggtt gttcgaatag gg
//
LOCUS       DATMXI010000301         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_44564, whole genome shotgun sequence.
ACCESSION   DATMXI010000301 DATMXI010000000
VERSION     DATMXI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44564"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VN444_05775"
     CDS             complement(<1..1212)
                     /locus_tag="VN444_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWR21354.1"
                     /translation="MGLKPATNPEVEAAEIELSIDGKSVMAKSGVSLYDVISSTGKII
                     PAMCYHYTFDPFGSCGMCLVMQEGKKAPVRSCSAKATAGMIIRTEGDDLFQARKKAVE
                     KHLSVHPLDCPVCDADGQCELQDTAFQHGVTNLANAKQKFIPEDTRSPALDFNMNRCI
                     ACAECINVCKDVLMIDALQFMKKGGFNQVVPKGDLALSCEFCGDCLAVCPVGAITNKF
                     SKYLYKPWQMKKTTTTCNYCGDGCQMYLETKDAEVVRVTSPLSWKNKWGDRADTAKGH
                     GGLCVRGRFGFEYIDSPARLKQPLLRKGNQLVESPWLETMHQVVDRFSEIQRKHGPDA
                     IAGLITARCTNEELYLFQKLMRAGFRTNHLDSSARYGHLNFVQASRHALGIGRNPNDW
                     EDLTRAKALLLI"
     gene            complement(1304..2548)
                     /gene="nuoD"
                     /locus_tag="VN444_05780"
     CDS             complement(1304..2548)
                     /gene="nuoD"
                     /locus_tag="VN444_05780"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWR21355.1"
                     /translation="MAIEDQRTTVYKIDPAHPESETLPTLRSEELLLNMGPQHPATHG
                     VLKVILELEGERLVKSTPVLGYLHRGVEKLAEDGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLMNITVPDRAEYLRTIVAEIQRIIGHQFWLGTQALDIGAMGVFFYCFRDREI
                     LMDWFDELCGARLTTSWYRIGGVERDFTPSLFGKLKQFLDYFPPKIDEYVVFLEKNRI
                     WLGRTKGVAIISAEDAVSFGLSGPTLRGSGVDYDLRKAEPYSAYAKCEFSVPLGKNGD
                     TYDRYWIRVQELYESIKIIRQCLEQMQDGPIMADVPSVTLPPKQRVFTNLESMIQQFK
                     LFSQGFDAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSRG
                     YMISDAVTIFGTYDIVMGECDR"
     gene            complement(2557..3060)
                     /locus_tag="VN444_05785"
     CDS             complement(2557..3060)
                     /locus_tag="VN444_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWR21356.1"
                     /translation="MSLQQLASRLEESFATGFVKAVEWRGELAVTVMRDRLHDVAQFL
                     RDDPAMDFDYIVHVSSVDWPDDDDRFEVVYEFYSIKKCHRIRLKTRVPESDCIVDSLT
                     DLWKGADFMEREVYDMMGIRFRHHPDLRRILLPDDYSEGYPLRKDFPLRGKGWRDTFE
                     FLDETTR"
     gene            complement(3071..3598)
                     /locus_tag="VN444_05790"
     CDS             complement(3071..3598)
                     /locus_tag="VN444_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWR21357.1"
                     /translation="MGLIQIGRHSKDSAPDIVTTTVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMLVAGTVCRRMAPVIRKLYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMLKR
                     VFVRQPDQVKPSHKI"
     gene            complement(3589..3972)
                     /gene="ndhC"
                     /locus_tag="VN444_05795"
     CDS             complement(3589..3972)
                     /gene="ndhC"
                     /locus_tag="VN444_05795"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWR21358.1"
                     /translation="MSGFELLSEYLTHYFPILFFVVVALFFGIWSLVISYLVQPKYPE
                     PEKLSSYECGSEPFSDARMPFPVRYYIFAMLFVIFDIEVIFLYPWAVVFTKIELIGLI
                     EMLVFIGLFLVAYVYAWRKGALEWD"
     gene            4589..>5848
                     /locus_tag="VN444_05800"
     CDS             4589..>5848
                     /locus_tag="VN444_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWR21359.1"
                     /translation="MANYSMKHVVSLMVWSLATPLLPLAWGADNLDSIRFGEKALAYS
                     SGSIQQSTPQDGFVNVITGDNQTTGDHMILGSRDVLYLRLNHPSDAALGDLFTIYRRT
                     HKVFHPATGQYLGHLVNRLAVVEVSQIDQELTTVQVIRAYAAVSPGDPVMRFVLPADE
                     EAAVDQPSAGNMEGQVVDFQSNMGTMNLVAQRNVVYLDRGREDGIRPGDRMKVIRTGG
                     SLPQRVVGELKILSTEDRTATALITKSTSRILKGDRFSRVAVPATEVIPVSQTPQQSL
                     TVSPTEAPSPSKFHVQNAAGETRISLSDFMKQIRFESGEATIKSEGYTVLDQLIAYLK
                     TAAGDQWIRVEGHADNMEIGPSLQSLYHTNWDLSKARARAVLRYLNEKGGIDSVKLSS
                     VGYGDTKPLASNATEVGRQQNRRVDIVL"
BASE COUNT         1407 a         1585 c         1510 g         1346 t
ORIGIN      
        1 gatgagtagc agggccttgg ctcttgtgag atcttcccag tcgtttgggt tccgtccgat
       61 tcccaaagcg tgcctggagg cctgtacgaa attgaggtgg ccgtagcggg cgctgctgtc
      121 aagatggttg gtccgaaacc cggcgcgcat gagtttctgg aagagataga gttcttcatt
      181 cgtgcaacgg gccgtgatta agccggcaat cgcgtcaggt ccgtgcttcc gttgaatttc
      241 tgagaaccga tcaaccacct ggtgcatggt ctcgagccac ggggattcaa cgagttgatt
      301 gcctttgcga aggagcggtt gtttcagacg tgccggacta tcgatgtact cgaacccgaa
      361 gcgcccccga acacagagtc caccgtgccc tttagccgta tcggccctgt cgccccattt
      421 gttcttccag gacagcggcg aggtcacgcg gaccacttca gcatccttgg tctcaaggta
      481 catctgacaa ccatccccgc agtaattaca cgtcgtggtt gttttcttca tctgccacgg
      541 cttgtacaaa tattttgaga acttgttcgt gatggcgccg accgggcaga cggcgagaca
      601 gtccccgcaa aactcacagg acagtgcgag atctcccttt ggcacgactt gattgaaccc
      661 cccctttttc atgaactgaa gcgcgtcgat catcagcacg tctttgcaca cgttgatgca
      721 ttccgcgcag gcaatgcaac ggttcatatt aaagtcgagc gcggggctgc gagtatcttc
      781 cggaataaat ttctgtttcg cgttcgccag gttcgtcacc ccatgctgaa aggctgtgtc
      841 ttgcagttca cattgaccgt cggcgtcaca gactgggcag tcgaggggat ggaccgagag
      901 atgtttttcc acggcctttt tcctggcttg gaagagatca tctccctctg tcctgatgat
      961 catcccggcg gtcgctttgg cagagcagga acgaacaggc gccttcttgc cttcctgcat
     1021 gaccaaacac atgccacaag agccgaatgg atcgaaggta tagtggtagc acatggcagg
     1081 gataatcttt cccgtgctcg agatgacgtc atagagcgag accccgctct ttgccatcac
     1141 agactttccg tcgatcgaca gttcgatctc ggctgcttcc acctcaggat tagttgccgg
     1201 ctttagtccc atattgctcc gaatgttgaa tgtttaattt tcaatgttga attgaaaaac
     1261 cgtcccacca ttcacaattg aacattagca ttaaacattt ctgttatctg tcacactccc
     1321 ccatcactat gtcataggtc ccgaagatcg tgaccgcatc ggagatcata tagcccctgg
     1381 acatatggtc gaaggcgccc atgtgaataa aagagggggc gcgaattttc aagcggtagg
     1441 gtttcccgcc gccggtgctg acgatgtaga atcccaattc ccccttgtgc gcttcggtcc
     1501 cgcaatagat ttcccccgga ggagcgtcga agccctggga gaacagcttg aattgctgga
     1561 tcatggactc caaattcgta aagactcgtt gcttcggcgg caacgtcacg ctcggcacat
     1621 cagccataat aggtccatcc tgcatctgtt ccaggcattg ccggatgatt ttgatgctct
     1681 cgtacagttc ttgaacgcga atccaatacc gatcatacgt gtcaccgttc ttaccgagag
     1741 gcacactgaa ttcgcatttc gcataggccg aatagggctc ggccttacgg aggtcgtaat
     1801 cgacacctga accacgaagc gtcggcccgc tcagcccgaa actcaccgcg tcttccgccg
     1861 agatgatggc cactcccttg gtccttccga gccagattcg gttcttttcg aggaacacca
     1921 catactcgtc tattttcggc ggaaaatagt ccagaaactg cttcagcttc ccgaacagcg
     1981 agggcgtgaa gtctcgctcg acgccgccga tgcgatacca actggttgtg aggcgcgcgc
     2041 cgcagagttc gtcgaaccag tccatcagaa tttcccggtc ccggaaacaa tagaaaaaga
     2101 cgcccatggc tccgatgtcg agggcctggg taccgagcca aaactgatgc ccgatgatcc
     2161 gttgtatctc cgccacgatg gtgcgtagat attctgcacg gtcgggcacg gtgatattca
     2221 taagcttctc gaccgcgcgg cagtatgcaa aattgttgta catcgcgcag acatagtcca
     2281 accggtcggt gtggggaata aattggtgat aagtgccgtc ttctgccagc ttctccacac
     2341 cgcggtgcaa atatcccagc acaggagtcg atttgacgag gcgttctcct tccaattcga
     2401 gaatgacttt caataccccg tgagtcgcag ggtgttgagg ccccatgttg agcaggagtt
     2461 cctctgaacg taacgttggg agagtctcgc tttccggatg agccggatcg attttgtaga
     2521 cggttgttcg ctgatcttct atggccatcg tctgtcctac cgagtcgttt cgtcgaggaa
     2581 ctcaaacgtg tcgcgccagc ccttgccacg gagcggaaaa tctttccgta gcggatagcc
     2641 ttctgagtaa tcgtccggta aaaggatccg tcggagatcc ggatgatgcc ggaagcggat
     2701 tcccatcatg tcgtacactt cgcgctccat aaaatccgcg cccttccaga gatccgtgag
     2761 ggaatccacg atacagtccg attctggtac cctggtcttg agcctgatgc gatggcactt
     2821 cttgatggag tagaactcat acacgacttc aaaccgatcg tcgtcgtcgg gccaatcgac
     2881 cgaactcaca tgcacaatat aatcgaagtc catggccggg tcgtcacgaa ggaattgtgc
     2941 aacgtcatgg agtcgatccc gcatcacggt cacggcaagc tcgccacgcc attcgacggc
     3001 ctttacgaag ccggtggcaa agctttcttc aagtcggctg gccagttgct gcaaggacat
     3061 tggtcgagac ctatattttg tggctaggct tgacctgatc cggttgtctc acgaacacac
     3121 gctttagcat gattcgctct tgtagcttga ggataccgtc aaagagcgcc tcaggcgtgg
     3181 gagggcaacc gggcacatag atatcgactg gcacgaaacg atcgacaccc tggacgacgc
     3241 tataactgtc atagatattg cctgacgtgg cgcaagatcc catcgcgatg acatatttcg
     3301 gctcaggcat ctgatcgtac agttttcgaa tgacgggggc catccgtcgg caaactgtac
     3361 cggccacgag catcagatcg gactggcgtg gcgaggcccg gaacacaccg gcaccatagc
     3421 ggtccatatc gtaccgtgac gagactgcgg caatcatttc gattgcacag caggccaggc
     3481 caaacgtcat aggccacagc gatcccttgc gagcccaatt gaccgccttt tccaccgttg
     3541 tagtgacgat gtcgggggcg ctgtccttgc tatgacgacc aatttggatc agtcccattc
     3601 cagagccccc ttgcgccaag catagacata ggccaccaaa aacaatccga tgaagaccag
     3661 catttcaatc aatccgatca actcaatctt cgtgaagacc actgcccatg gatacaaaaa
     3721 gatgacctca atatcgaaaa taacaaagag catcgcgaaa atgtaatatc gaaccgggaa
     3781 aggcattcga gcatctgaaa atggctctga tccgcactca taggaagaga gcttttccgg
     3841 ctctggatat ttcggctgga ccagataact gataaccagc gaccagatgc caaaaaacag
     3901 cgcaaccaca acaaagaaca agatcggaaa gtagtgggtc agatactcgg agagtaactc
     3961 aaagccgctc attgctctga cctttcaaca ccttgagata caaggaaagc ttgatattat
     4021 cttctgggtt aagggatagc aagcgttcaa aggacctgct ggatcgaccc aatagtccta
     4081 gacactgctg cttgacaagt catcatccag cgatgagaaa tcaacagctt atggaaactt
     4141 ttctgaagtt cgtatgggtg gagccgaagc cgaagggatt ccatctcccc acgacctgct
     4201 tatgcctgtc ggagcagatc tacttatgca gaattctccg cttcgtgact gtggtaggtt
     4261 ccatacgatc tgatgacata cgcaaactgg agggcggcat accagaagtt tgatcccact
     4321 caagctccag agccgagaag gccttggctt tcttgcgtcg gtgaatctgg gtcttgatac
     4381 ggggaggatc tcgacgttga gcatcatgga cgggcacctg gtcaaattcc ataaatggaa
     4441 cccctttgtc agcagccaaa atctgaatca ggctagcatc gagtaaatcc gcgggtcaag
     4501 cagtgaaaga ccctaatatc cttgacaata caagactatt tgctatggtt ttcacagcaa
     4561 tacttgcctc acatgaagga caaccattat ggctaactat tcaatgaaac atgtcgtctc
     4621 cctgatggtc tggagcttag ccacaccgct ccttcctctg gcctggggag cagacaatct
     4681 cgattcgatc agattcggag aaaaggcttt ggcctattca tccggctcca tccagcagtc
     4741 gactccacag gatgggttcg tgaatgtgat aaccggcgac aatcagacaa ccggagatca
     4801 catgatattg ggaagccggg atgtcctcta cctccggctg aaccatccca gcgatgccgc
     4861 cctaggagat cttttcacta tctacagacg aacgcacaag gtattccatc cggccaccgg
     4921 ccaatacctc ggtcatctag ttaatcggct cgcagtggtt gaggtcagcc aaattgatca
     4981 ggagctgacc actgttcagg tcattcgggc ctacgcagct gtatcacctg gtgatccggt
     5041 gatgagattc gtacttccgg ccgacgagga agcagcagtg gaccagccct ctgccggtaa
     5101 tatggaaggg caagttgtgg actttcaatc caacatgggg acaatgaacc ttgtggcaca
     5161 aaggaacgtc gtgtacctgg accggggacg ggaagatggg atcagaccgg gggatcgaat
     5221 gaaggtaatc cggaccggag ggagtcttcc ccagcgagtc gtgggtgaat taaaaatcct
     5281 gtccacggaa gaccgaaccg ccacagcctt gattacaaaa tcaacctccc gcattctcaa
     5341 gggtgatcgc ttcagtaggg tcgcggttcc tgctactgag gtcatcccgg tctctcaaac
     5401 gccccagcag tcattaactg tgtctccaac tgaggctcct tccccaagca agtttcacgt
     5461 acagaatgct gctggcgaaa ctcgcatcag tctcagtgac ttcatgaagc agattcggtt
     5521 tgaatccggc gaagccacaa tcaagtccga gggctatacg gtactcgatc aactcattgc
     5581 gtatctcaag accgctgccg gtgatcagtg gattcgagtc gagggtcatg cggataacat
     5641 ggaaatcggc ccttcgctcc agtcgctcta ccacacgaat tgggacttgt cgaaagctcg
     5701 ggctagagct gtccttcgct acttgaacga gaaaggcggg atcgattcgg tgaaactgtc
     5761 atcggttggc tatggcgata cgaagccgct tgccagcaat gcgacagaag tcggtcgaca
     5821 gcagaaccgt cgtgtggata tcgtactc
//
LOCUS       DATMXI010000302         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_221422, whole genome shotgun sequence.
ACCESSION   DATMXI010000302 DATMXI010000000
VERSION     DATMXI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221422"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VN444_05805"
     CDS             complement(<1..636)
                     /locus_tag="VN444_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWR21360.1"
                     /translation="MPLLDLRAQYLTIKDEVRAELDEVYESQHFILGPKVAALEQAIA
                     RTCGAAYAVGVSSGTDALLSALMALGLSTGDEVITTPYTFFATAGSIVRAGARPIFID
                     IDPASFNLEPSQVQDRITRRTKVLLPVHLFGRCAEMEPLRTLAAQRHLAIIEDAAQAI
                     GARTAEGGKAGGIGTLGCLSFYPTKNLGGFGDGGMVLTSDPRLADILRSLRQ"
     gene            946..1380
                     /gene="speD"
                     /locus_tag="VN444_05810"
     CDS             946..1380
                     /gene="speD"
                     /locus_tag="VN444_05810"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HWR21361.1"
                     /translation="MQALGKHLLVELHGCNPELLKKVDVIKEILVCAANACKATIVDT
                     SFHEFNPFGVSGVVVIAESHISIHTWPEYRYAAVDIFTCGDVIKPDVAVEYIATRFQC
                     KKPSVVEMRRGIIPGHHGKLAHKLSASMEKAVDADQELSLVH"
     gene            1349..2266
                     /gene="speE"
                     /locus_tag="VN444_05815"
     CDS             1349..2266
                     /gene="speE"
                     /locus_tag="VN444_05815"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HWR21362.1"
                     /translation="MPIKNSRWFIEALSPEEGHLHGIRRVLFSTQTPFQSLDIMELGS
                     YGKALILDDKVQSSLLDEFIYHEALVHPAMFSHPAPKKVFIVGGGEGATLREVLRHPT
                     VERALMVDIDEEVVNRCKELLPEWHRGAFDDPRAEVRFLDARRYLEETDERFDVIIID
                     ISEPVEEGPAYLLFTREFYQIVHRCLTDQGIIALQAGSVSLSNLSCFTAIHETLRTAF
                     PVVAPYWASVPSFALPWGFSVASKRPDPRALGPDTIDRLIAERIGPDLRYYDGLTHQM
                     SFLLPKYVRRHLEEEKRIIEDNHPLFTFH"
     gene            2387..>3359
                     /locus_tag="VN444_05820"
     CDS             2387..>3359
                     /locus_tag="VN444_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWR21363.1"
                     /translation="MVPERQYRTPLFDAMVNLAESRKISFHTPGHKSGKGISTRFRKY
                     VGPKIFTIDLTTLDEVDCLQRPVGVIKEAQELAAEAYGADHSFFLINGTTGGNHAMIL
                     STASPGDEILIARNAHKSILTGIILSGATPRFFLPAFDQDLGMLLNATVEGVERAMAQ
                     YPKAKVLSLTSPNYYGVAADLRAIVSLAKSRGILVLVDEAHGPHLHFHQGLPTSALDA
                     GADLCVQSTHKILGGMTQSSMLHLRGPDVDYPRVVKLLLLLQSTSPSYILMASLDLAR
                     MQMATEGRKLLAKAIELAEEARARINRIAGLRCLDAAQVEAPQSGDTV"
BASE COUNT          682 a          979 c          976 g          722 t
ORIGIN      
        1 ctgcctgagg ctcctgagaa tgtctgccaa tctaggatca ctggtcagca ccatgccgcc
       61 atcgccaaat ccacccagat tttttgtcgg gtagaaagag aggcagccga gcgttcctat
      121 tccgcctgcc ttcccaccct cagccgtcct ggcgccgatt gcctgagcgg catcttcgat
      181 gatggcgagg tggcgttgcg ccgccagcgt acgaagcggt tccatctctg cgcagcgacc
      241 gaacagatgg acggggagga gaaccttggt ccgccgcgta atccgatcct gtacctgaga
      301 cggttccaga ttgaagctgg cagggtcgat gtcgatgaag atcgggcgcg ccccagccct
      361 gacgatagag ccggcagtcg caaagaaggt gtacggagtg gtgatgacct cgtcgcccgt
      421 gctgaggccg agcgccatga gggcgctcag gagggcatcg gtgccggatg agacccccac
      481 ggcataggcg gctccgcagg tacgagcgat cgcctgttcc agggcagcca ccttcgggcc
      541 gaggatgaaa tgctgacttt cgtagacttc atccaattcg gcacgcacct catccttgat
      601 cgtgaggtac tgcgcgcgaa ggtccaggag tggcacgttc aagtcggttc cccgatgaga
      661 cctacccaag gtagggaaga cgttgatggt tcaccgtgac agttgcccgt ccacggccgc
      721 tgcgtagtcc atggtagacc gtcagcgatg atccatcgat caggggtggc gtgctagagt
      781 ggacccattt ctacccaaca aaggaccggg ttgtcaacca gaatttatgt tagcccccca
      841 tttttttctt gcaattttgt cgaaacgcgg tataacgcgc ttgaacaatg acgcaccacg
      901 gaggtggggc atcgattatc aacttttttg aaggggggta gatcgttgca agcactcggg
      961 aaacacctgt tggtggaact acacggttgt aacccggagc tgctcaaaaa agtcgatgtt
     1021 atcaaggaga tcctggtttg cgccgcgaac gcctgcaagg ccacaatcgt tgacacctcc
     1081 ttccacgagt tcaatccttt cggtgtgagc ggcgttgtgg tgatcgccga atctcacatc
     1141 tctatccata cctggcccga gtatcggtat gcggcggtgg acatcttcac ctgtggagat
     1201 gtcattaagc ccgatgtggc ggtggaatat atcgccaccc ggtttcagtg caaaaagccg
     1261 tcggtcgtcg agatgcggcg gggcatcatc cccgggcacc acggaaagct tgcccacaaa
     1321 ttgagtgcgt ccatggagaa ggctgtcgat gccgatcaag aactctcgct ggttcattga
     1381 agccttgagc cctgaggagg ggcatctgca cgggattcga cgagtcctgt tttccacaca
     1441 gacccccttt cagtccctcg acatcatgga gcttggcagc tatgggaagg cccttatcct
     1501 tgatgataag gttcagtcaa gcctcctcga tgagttcatc taccacgagg cattagtcca
     1561 tccggcaatg ttcagtcatc ccgcgcccaa aaaggtattt atcgtgggcg gtggggaagg
     1621 ggccaccttg cgggaagtcc tccggcatcc cactgttgag cgggccctga tggtggatat
     1681 cgatgaggag gtcgtcaacc gatgtaagga gttgctgccc gaatggcacc ggggcgcctt
     1741 cgacgatccg cgcgccgagg tccgcttcct tgatgcccga cgatatctgg aggagacaga
     1801 cgagcggttc gacgtgatca tcatcgacat ctcggagccg gttgaagagg gcccggcata
     1861 tctcctcttt acgcgggagt tttatcaaat cgtgcaccga tgcctgacgg atcaggggat
     1921 catcgcgttg caagcgggtt cggtcagtct ctccaacctg tcctgtttta ccgccatcca
     1981 cgagaccttg cggaccgcat tccccgtcgt ggccccctac tgggccagcg tccccagctt
     2041 tgcccttccc tggggattct ccgttgcctc caagcggcct gacccacgag cgctgggacc
     2101 ggatactatc gatcgcctca tcgccgaacg gatcgggcct gacctgcgat attacgatgg
     2161 tcttacgcat cagatgtctt ttctcctccc gaagtatgtg cgccggcacc ttgaggagga
     2221 gaagcggatc atcgaggata accaccccct ctttaccttc cactgagaga gtacagttca
     2281 tagtctaggt atggctagat tgaattgtac gatcttccag cgattgggtg tcatctatga
     2341 actctgaact acgaactctg aacgcctttt cccgctcacc tttctaatgg ttcccgaacg
     2401 acagtacagg acgcccctct tcgacgcgat ggtcaacctc gcggagagtc gaaagatctc
     2461 gttccacacc cctgggcaca agagcggtaa gggcatctct acgcgcttcc gaaagtacgt
     2521 cggcccgaag atctttacca ttgacctgac aacgcttgac gaggtcgatt gtctgcagcg
     2581 gccagtgggg gtcattaagg aggcccagga gttggccgcc gaggcgtatg gagctgacca
     2641 ttccttcttc ctgatcaacg ggaccaccgg gggcaatcat gcgatgatcc tgtctaccgc
     2701 gagccctgga gacgagatcc tcatcgcacg gaatgcccac aaatcgatcc tgaccgggat
     2761 catcctcagc ggcgcgacac cgcggttctt cctgccggcc ttcgatcagg atttgggcat
     2821 gctgttaaac gcaacggttg aaggggtgga acgcgccatg gcgcagtatc cgaaggccaa
     2881 ggtcctctcc cttaccagtc ccaattacta cggggtggcg gccgatctgc gcgccatcgt
     2941 ctccctcgcc aagagccggg gtatcctggt cctggtggat gaggcccacg gcccccacct
     3001 tcattttcac caggggctgc ccacatcggc gctcgacgct ggagccgatc tctgtgttca
     3061 gtccacccat aagatcttgg gggggatgac ccagtcctcc atgctgcatc tgaggggtcc
     3121 agatgtcgat tacccgaggg ttgtaaagct gttactcctc cttcaaagta cgagcccctc
     3181 ttacattctg atggcctcgt tggatctggc gcgcatgcaa atggcgacgg aagggcggaa
     3241 actcctggcg aaggcgatcg aactggcgga ggaggcgcgg gcgcggatca accgtatcgc
     3301 cggactgcgg tgcctcgacg ctgcgcaggt cgaggcaccg cagtccggcg atacggttg
//
LOCUS       DATMXI010000303         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_145352, whole genome shotgun sequence.
ACCESSION   DATMXI010000303 DATMXI010000000
VERSION     DATMXI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145352"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2859)
                     /gene="alaS"
                     /locus_tag="VN444_05825"
     CDS             complement(199..2859)
                     /gene="alaS"
                     /locus_tag="VN444_05825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWR21364.1"
                     /translation="MTGGAIRERFLRFFERNDHTVVASSSLVPADDPTLLFTNAGMVP
                     FKGVFLGTDPRPYRRATSIQKCLRVSGKHNDLENVGRTARHHTFFEMLGNFSFGDYFK
                     ESAIEYGWAFLTRELELPVDRLWATVYKDDDAAFDLWQKLAGLPPDRVVRLGDKDNFW
                     TMGETGPCGPCSELIFDQGPTVGCGRPTCSIECGCDRYLELWNLVFMQYNRDAAGTLT
                     PLPKPSIDTGAGLERMAAVCQGVNSNFETDLIRPLIATVEELSQKRYGADEKDDVSMR
                     VIADHARAVTFALADGVLPSNEGRGYVLRRILRRALRHGRLLGLDEPFLASATDKVID
                     LMTEAYPDLPASRAHVARFAWIEEDRFGIVLREALPRLHDLIQNVKTRTAERAILSGS
                     TLFELYDTYGVPRDLMDEIAIEQSVECDWGGFETELRHQREKSRAHLKTFGDVQGVAE
                     AFRDLAKERRTVFLGYERLDVQAAVVAVLADGTQVERIDAGQEGEVVLDQTPFYAESG
                     GQVGDTGYLRGEALIAEVLDTKRPLTGLIVHRVRVKRGDLRRGQRLLASVDVDRRTTT
                     IKNHTATHLVHAALRQILGDHVRQEGSLVAPDRLRFDFRHYGPLSPAEITQIEEMVNT
                     KLWANQPVVIEEMRMDEALAKGALAFFGDKYGDEVRVVSIADFSIELCGGAHTKATGE
                     IGLFKISHEAGVAAGVRRIEALTGPGAFQYLKREGEVLRESADRLKSKPLEVPEKVDR
                     LFDSTRALEREVQHLRAKLGAEMVEELLKKAIQVSGVTVVAGLAEWCDQRALRELADR
                     LRSRIGSGVIVLATSSDGRVSWVAAVTPDLTSRLHAGKLVKEVASITGGSGGGRADLA
                     EAGGKDPDKLDRALQLVPELVGRFPASTRC"
     gene            complement(2880..>3130)
                     /locus_tag="VN444_05830"
     CDS             complement(2880..>3130)
                     /locus_tag="VN444_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HWR21365.1"
                     /translation="GSYRLGKELEVKGVEEQLVREVLEDLYEEGEEPAARRAIAGKLS
                     ALGRLPASARIIRVGRFLHRRGFPSELIWRLLHEEQQG"
BASE COUNT          622 a         1067 c          835 g          606 t
ORIGIN      
        1 ttatttagtc gtttagggct gaaggctgaa agttatacat taactcacag gaccacaaat
       61 caatctaatt cgaacgcccg aagttttgca taatcgcgca tgacttaccc cgaaaagcct
      121 tcagcctatc tacctgggta attaagtgat tctactcatt cataccatgg ctggcggcga
      181 ccgccaaggg aaataaggtc aacaacgggt tgacgcggga aaccgtccga ccagctccgg
      241 gacaagctgg agcgcccgat ccagcttgtc aggatccttc ccgcccgctt cggcgagatc
      301 ggcccggccg cccccgctcc caccggtgat acttgccacc tccttcacga gcttgccggc
      361 atgcagccgc gaggtgaggt caggtgtcac cgccgccacc cagcttaccc gtccgtccga
      421 ggacgttgcc aggacgatca ccccgctgcc gatcctggac ctcagtcgat ccgcgagctc
      481 ccgtagagcc cgctgatcac accactcggc caggcccgcc actaccgtga cgccggagac
      541 ttggattgct ttcttcaaca actcctccac catttccgca cccagcttgg cccgaaggtg
      601 ctggacctct cgctcgaggg cgcgcgtaga gtcgaagagg cggtcaacct tttccggaac
      661 ttcaagcggc ttgctcttca gccgatcggc cgattcccgc aggacctccc cctcacgctt
      721 caggtactga aaagccccag ggccggtcag ggcctcgatc cgtcgtacgc cggcagcgac
      781 gccggcttca tgcgagatct tgaacaggcc gatctccccc gtagccttgg tgtgggcccc
      841 tccgcacagc tcgatactaa agtcggcgat actgaccacc cgcacctcgt ccccatactt
      901 gtcgccgaaa aaggccaggg cgcctttggc cagcgcctcg tccatccgca tctcttcgat
      961 cacaaccggc tgattggccc agagcttcgt attgaccatc tcttcgatct gagtgatctc
     1021 ggcgggactc agaggcccgt agtgccggaa gtcaaaacgc aagcggtccg gcgcaaccag
     1081 cgagccttcc tgtcgtacat ggtctccgag gatctgccga agcgccgcgt ggacgaggtg
     1141 ggttgctgta tgatttttta tcgtcgtggt gcgccgatcc acatcaaccg aagccaggag
     1201 ccgctgacct ctcctgaggt cgccccgctt cacccggacc cggtgaacga tcaatcctgt
     1261 aagcggccgt ttggtgtcca ggacctccgc gatgagcgcc tcacctcgaa ggtacccggt
     1321 gtcacccacc tgtccaccgg attctgcata gaagggggtc tgatcgagca cgacctcgcc
     1381 ctcctgcccc gcgtcgatcc gctcgacctg tgtcccgtca gctagaaccg caacgaccgc
     1441 cgcctgtaca tcgagacgct cgtagcccag gaataccgtc cgcctctcct tcgcaaggtc
     1501 acggaaggcc tctgcgaccc cttggacgtc tccaaatgtt ttcagatggg ctcgcgactt
     1561 ttcccgttgg tgtctcaact ccgtctcgaa cccgccccaa tcgcattcca ccgactgctc
     1621 aatggcaatc tcgtccatca ggtcccgagg cactccgtat gtatcgtaca actcaaagag
     1681 cgttgaccct gaaaggatgg cccgctccgc tgtccgggtc ttcacgttct ggatcagatc
     1741 atgaagccgg ggcagcgcct cgcgcagcac gataccgaac cggtcctcct cgatccaggc
     1801 gaaacgcgcg acatgcgccc ggctcgccgg gagatccgga taggcctccg tcatcaggtc
     1861 gatcaccttg tcggtcgcag aggccaggaa cggctcgtcc agtccaagga gacggccgtg
     1921 tcggagcgcg cgccggagga tccgtcgcag tacgtagccc cgcccctcat tgcttggcag
     1981 gacaccgtcg gccaacgcga aggtgaccgc tctcgcgtga tcggcgatca ccctcatcga
     2041 cacatcatcc ttctcgtccg ccccgtaacg cttctgcgaa agctcctcga ccgtcgcgat
     2101 tagcggccgg atcaggtcgg tctcgaaatt actgttgacc ccttgacata ccgcggccat
     2161 tcgctccagc ccagcgccgg tgtcgatgct cggcttcggc aggggcgtaa gggttccggc
     2221 ggcatcacga ttgtactgca tgaacaccag attccagagc tccagatagc gatcgcaccc
     2281 gcactcaata ctacaggtcg gccgtccgca gcccaccgtc ggaccctggt cgaagatcag
     2341 ctcagagcag ggcccacatg ggcccgtctc gcccatcgtc cagaaattgt ccttgtcccc
     2401 caggcgaacg actcgatctg gcggcaggcc ggcaagcttc tgccacaggt cgaacgccgc
     2461 atcatcatcc ttataaacgg tggcccagag ccgatctaca ggtagctcga gctcgcgcgt
     2521 caggaacgcc cagccatatt caatggcact ctccttgaag taatcgccaa aggagaagtt
     2581 gccgagcatc tcgaaaaagg tatggtggcg agcggttcga ccgacattct ccaggtcgtt
     2641 atgtttaccg ctgacgcgca aacacttctg gatcgaagtg gcgcgacgat agggacgcgg
     2701 atcggtccca agaaaaactc ctttgaacgg caccatcccc gcattggtga agagcagcgt
     2761 cggatcgtcg gctgggacca gtgaggaact ggcaaccacc gtatggtcgt tccgctcaaa
     2821 gaatcgcaag aaccgctcgc ggatcgctcc gcctgtcaac cccagcatga cgtgttccgc
     2881 tacccctgct gctcctcgtg aagcagtcgc cagataagtt cgcttggaaa ccctctccgg
     2941 tggagaaacc gccccactcg gatgatccgg gctgaagccg gaagacgccc aagcgccgag
     3001 agctttcctg ctatagcacg gcgggctgcc ggctcttctc cctcttcgta aaggtcctct
     3061 agaacctcac gtacgagctg ttcctcaacc cccttgacct cgagctcctt gccaagtcga
     3121 tacgacccca
//
LOCUS       DATMXI010000304         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_24382, whole genome shotgun sequence.
ACCESSION   DATMXI010000304 DATMXI010000000
VERSION     DATMXI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24382"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VN444_05835"
     CDS             <1..647
                     /locus_tag="VN444_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HWR21366.1"
                     /translation="EEQKLQMPLFPKPFTLTPQPFRVVAYDCGIKLNILRKLVEAGCD
                     VTVVPPDTPASTVMGLAPDGVFLSNGPGDPEGVPYLINNVQKLIGATPIFGICLGHQI
                     LGLALGGRTYKLKFGHHGGNQPVKDLTTGKVEITTQNHGFALDIASIPDKEIELTHIN
                     LNDQTVEGMRHRCLPIFSVQYHPEASPGPHDAAYLFQRFVDLMTQERNRAGAQA"
     gene            655..849
                     /locus_tag="VN444_05840"
     CDS             655..849
                     /locus_tag="VN444_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HWR21367.1"
                     /translation="MKVKYDQEVDVLTIEFSDAQVEESDQDKPGVILDYDKDGNIVGM
                     EILNASKRVQNPKSVEYAVA"
     gene            884..>2345
                     /gene="carB"
                     /locus_tag="VN444_05845"
     CDS             884..>2345
                     /gene="carB"
                     /locus_tag="VN444_05845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWR21368.1"
                     /translation="MPKRTDIESILIIGSGPIVIGQACEFDYSGTQACKALREEGYRV
                     ILVNSNPATIMTDPEMADRTYLEPLTVPILSQIIAREKPDALLPTLGGQTGLNLAVAL
                     AEEGILDRYGVEMIGAKLPAIKKAEDRDLFKQAMQKIGVEVAESGHARSLEEARAIVE
                     RIGYPAIIRPSFTIGGTGGSIVYNRDELDEQVQWGLNVSPVHQVLVEASVIGWKEFEL
                     EVMRDLKDNVVIICSIENFDPMGVHTGDSITVAPAQTLSDKEYQLMRDAAIAIIREIG
                     VETGGSNIQFAVNPENGRMLAIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     INNDITKETTACFEPTIDYCVVKFPRFSFEKFAGADETLTTQMKSVGEAMAIGRTFKE
                     ALQKVIRSLEIDTYGLESRIFKGKTVRGAAVSEADLQLVRDRLRIANWERVFYIADAL
                     RLGMPVQEVHKLTAIDPWFIENIKEIVDFEDRLIGLA"
BASE COUNT          511 a          667 c          718 g          449 t
ORIGIN      
        1 gtgaagaaca gaagctccaa atgcctctct tccctaaacc ctttacccta acccctcaac
       61 cattccgtgt ggtggcctac gactgcggga tcaagttgaa catcctgcgg aagttggtgg
      121 aggcagggtg cgacgtgacg gtggttccgc cggacacccc tgcgtccacg gtcatgggtc
      181 tggccccgga tggggtcttc ttgagtaatg gccccggcga cccggagggc gtgccgtacc
      241 tgattaacaa cgtacaaaag ctgattggcg ccacgccgat cttcggcatc tgcttgggcc
      301 atcagatcct gggtctcgcc ctcgggggac ggacctacaa gctgaagttc ggtcatcacg
      361 gcggcaacca gcctgtcaag gatctgacga ccgggaaggt ggagatcacc acccagaacc
      421 acggctttgc cttggacatc gcctcgatcc cggataaaga gatcgagctg acccacatca
      481 acctcaacga ccaaaccgtc gaggggatgc gacatcgttg cctcccgatc ttttcggtgc
      541 aataccaccc ggaagcctcc cctggtcccc atgatgccgc atacctcttt cagcgcttcg
      601 tagatcttat gacgcaggaa cgcaatcgag cgggcgcaca agcttgaggg acccatgaag
      661 gtcaaatatg accaagaagt ggatgtactc acaatcgaat tcagtgacgc acaggttgag
      721 gagagtgacc aggacaaacc cggggtgatt ctggactacg acaaggacgg caacattgtg
      781 ggcatggaaa tcctcaacgc gtccaagcgg gtccagaatc ctaaatccgt agagtacgct
      841 gtcgcttgac gcgtgaggtt cgatccgtgg gaatcttgta gctatgccga agcgaaccga
      901 cattgagagc atcttaatta tcggctcggg cccgatcgtc atcgggcagg cgtgcgagtt
      961 cgactactca gggacccagg catgtaaagc ccttcgggaa gaaggctacc gggtgatcct
     1021 ggtcaactcg aatccggcca cgatcatgac cgacccggag atggcggacc ggacctacct
     1081 ggagccgctg accgtgccta tactctcgca gatcatcgcc cgggagaaac cggatgcgct
     1141 gttgcccacg ctcggggggc agaccggcct caacctggcc gtcgcgttgg ccgaggaggg
     1201 aatcctggac cgatacgggg ttgagatgat cggggcgaag ctgccggcca tcaagaaggc
     1261 cgaagaccgc gatctcttca agcaggcgat gcagaagatc ggggttgagg tggctgagag
     1321 cggccacgcc cgttccctcg aagaggctcg tgctatcgtc gagcggatcg gttacccggc
     1381 gatcatccgt ccttcgttca cgatcggcgg caccggcggc agcatcgtct acaaccggga
     1441 tgagctcgac gagcaggtcc agtggggcct caatgtgagc cctgtccacc aggtcctggt
     1501 cgaagcctcc gtgatcggct ggaaggagtt cgagctggaa gtgatgcgcg acctgaagga
     1561 taacgtcgtg atcatctgtt ccatcgagaa cttcgacccg atgggggttc acaccggcga
     1621 ctcgatcacg gtggccccag cgcagaccct gtctgacaaa gagtaccagc tcatgcggga
     1681 cgccgcgatc gctatcatcc gggagatcgg tgtcgagacg ggcggcagca acatccagtt
     1741 cgccgtcaat cccgagaacg ggcggatgct ggcgattgag atgaatccaa gggtttctcg
     1801 atcctcggca ttggccagta aggcgaccgg gttccccatc gcgaagatcg ccgccaagct
     1861 ggctgtgggt tatacgctgg atgagatcaa taacgatatc acgaaggaga cgacagcctg
     1921 ttttgagccg accatcgact actgcgtcgt caagttccct cgattctcgt ttgagaagtt
     1981 tgccggtgcg gacgagaccc tgacgacgca gatgaaatcg gttggggagg cgatggcgat
     2041 cggccggacc tttaaggagg ctttgcagaa ggtgatccgc tcgctcgaga tcgataccta
     2101 tggactcgag agccggatct tcaagggtaa gacagtgcgg ggtgcggcgg taagcgaggc
     2161 cgacctccag ttggtccgag accgccttcg cattgccaat tgggagcggg tcttctacat
     2221 tgccgatgcg ctccggctgg ggatgcccgt gcaggaggtc cacaagctca cggccattga
     2281 tccctggttc atcgaaaaca tcaaagagat cgtggacttt gaggaccgat tgatcggcct
     2341 cgctt
//
LOCUS       DATMXI010000305        10319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_28781, whole genome shotgun sequence.
ACCESSION   DATMXI010000305 DATMXI010000000
VERSION     DATMXI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28781"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VN444_05850"
     CDS             complement(<1..230)
                     /locus_tag="VN444_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HWR21369.1"
                     /translation="MSEITVIIGLGLIMGLVLVLPFSVRWVEEELEAFLLVMGCLAVT
                     MSGLWSRHLVGEALTEPVKISAAVLVFGLLFR"
     gene            complement(665..3208)
                     /locus_tag="VN444_05855"
     CDS             complement(665..3208)
                     /locus_tag="VN444_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWR21370.1"
                     /translation="MADIENSRRVLSYQPSTGKLAETGDSVRILTYNTYLLSRIFLCK
                     WDPLCHLGDANVEPRAEQLGAVVAMTNEKYDIVAFNEVWDEDTKDVLEERLGGIYPSY
                     VKYISKSGPDMIQLKKDWGFLGGNFEDSGLMLFSKFLFQRLPFVKNDYEATDFKPWWI
                     KKRLAFKEFHDCAFAFADCRAAKGVALVQVELPSKRSLHVLLTHTQADSGDSTHPEVR
                     KNQLADMQDIVETTLGKTFASWTDTEKLVALGDLNIDGLGGVSQALGLPADFLADATQ
                     EWVDRFKAPAQAGVTPLYDSWAETTSPQDQGHSRYNKNERLDYILVSRPIADTYAGQI
                     LNDNEGEIPWPPADLSVTCVQHIWIPQELEGLSNHRPVAADINLFAPQCNPRLAQTVG
                     DADLGTIGKGLKSTSWLHHELKHRGSMQWFRLEAPGTYAIAISGINAADTPLLHYEIY
                     SPDNLSVPLAGAYQLENTKLVVCGTGVPVQERIPGPATTAAPVSECGLVDAQKFVLPT
                     GPHYVRVFSKQRTWQGKYAVAFYKFNCTARDDACDLLPNSPQEFAFLSGKPLNAEDAA
                     WFRVRIEDQAGSGQAQSLKFSSRHLSGALAAPMIDFVQDDGVTPLADLSGNPLIGPLV
                     QDNGATYNSQTNQNQTLYLRIKRPVANLQKHTLAVSWRTNLTLFGGSMVGPGIPVLIC
                     KDETNGTLGNEYSTDEIHMEVNVDGQGWTKIPASGYVEFDCNDWEDAKIWGEQLGVIG
                     FLDSVKIRMVEEDDSGDDDTSPSVSLEAWDMNMDMDQIPDNLKTADRKTKLQWEWEGG
                     EYHLGTFNMSRWRIKNGSSGNRVGMAGAGTDFAEGVGTHRAAAGKEHGK"
     gene            complement(4201..4986)
                     /gene="otsB"
                     /locus_tag="VN444_05860"
     CDS             complement(4201..4986)
                     /gene="otsB"
                     /locus_tag="VN444_05860"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWR21371.1"
                     /translation="MEWLWSQWPPVAAEIVSSSHTVLLLDYDSTLTWFASCSEQATLP
                     VSTREVLQELAGHPRLTVAVISGRRLDDLRRLVRVRNLIYIGHYGLEIWHDGRQVKMN
                     APPPFQEAVTRIRSQLTNLVAEIPGALVEDKGLSISLHYRLVPHELAPHLVGVFVRDI
                     LPVARLSGLTVLHGKNVIELRPQLNRTKGHAALWVMQHSRRRSALPIYIGNDRTDEDA
                     FRALAEGITIRVGALDGSEAHYYVRDVKEVMALLQWMAATCST"
     gene            complement(5057..5707)
                     /locus_tag="VN444_05865"
     CDS             complement(5057..5707)
                     /locus_tag="VN444_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR21372.1"
                     /translation="MKRIRVLLLDDHILFREGLNRLLQSEPDFEIVGVCGTSAEALEI
                     LSRVPVDVVLLDFDLGEDYGSRLILSARGGGYTGKILMVTAGMNAAESSVALKLGASG
                     IFLKHSPPDALVTAIRRVVSGEAWVDQRIIQLMADAVNQPEEQEDHKRLTEREEQVLQ
                     AVFEGLTNKEAATQLGVTEGAVKATLQHLFQKTGVRTRSQLVRVAIEGWRRAGRKF"
     gene            complement(5679..7142)
                     /locus_tag="VN444_05870"
     CDS             complement(5679..7142)
                     /locus_tag="VN444_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21373.1"
                     /translation="MGWAMIFPPFVARRHIVLTCAAILIGTVAVIDWRVDIDVSFGFL
                     YVFPVMLAASVLPRGAIALMAVLCTVLSDLFDPFPLTPSVGLLRDLSVFAALTGAGLF
                     VHEVIRSRRGEMEHLQRIEQEVVARRQAEEQLAFLIDSSPVAILTMAGDGAILQANSS
                     AHRLFGAPNGNLPGRNISRYVSALGAVPSVENTRQTFRTEMQCRGAREDGSIFPARVF
                     FSTYTTAAGPRLAALIVDASEELREREESSLEHLLAGSRVVVGAVSHEIRNVCSAIAV
                     LYENLTRDGRLLANKDFEALGSLVETLNKIASLEFKHSRSGARTTQIDGADLAELFDS
                     LRIVLGPSCEGAGITVDWEIPEGLPPVWADNHLLLQALLNLTKNAERALERADVKRIA
                     ISVSVGKGSVSIRVTDTGPGIGSAEKLFRPFQAGAESTGLGLFLSRALVRSFRGDLRY
                     DPTVPGCSFVIDLAVARSTEMNDGSTGTHEANTGAVA"
     gene            7937..8818
                     /locus_tag="VN444_05875"
     CDS             7937..8818
                     /locus_tag="VN444_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HWR21374.1"
                     /translation="MDEREIFMAKQAMQSALMKVPVMLVLMFVAILVGFLILHPWVQI
                     GAGERGVVLNFGAVQEDVLGEGLHFRIPIMQRIIPMDVKVQKAVTDAASASADLQDVT
                     LAVALNYHILPDKANVVYQTLGIQFKERIIDPAIQEVMKAVSARYTAEELITKRPAVS
                     EAIRAALSERLLVYNIVVDAFSIVTFSFSRVFTEAIEAKQTAEQLALKAKRDLDRIKV
                     EAEQTIAAAKAEAESLRLQKANISPDLIELRKIEANLRAIEKWNGILPQVTGAGAVPF
                     IGVGGTQESKGRKEKDG"
     gene            complement(9281..9748)
                     /locus_tag="VN444_05880"
     CDS             complement(9281..9748)
                     /locus_tag="VN444_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR21375.1"
                     /translation="MKDGATKSCVQAYNAQAVVDGQAQIIVAAAVTQRANDTAQLVPM
                     LQHVEANLDRKPTQVSADAGYFSAPHVTDMTLQGIDLYVPPDRQKHGELSPSDSDPPP
                     PDAPVIAQMRHKLRTVAGQAIYALRKAIVEPVFGQIKAPGGASGALRSGDRPR"
     gene            complement(9745..9942)
                     /locus_tag="VN444_05885"
                     /pseudo
     CDS             complement(9745..9942)
                     /locus_tag="VN444_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(10075..>10319)
                     /locus_tag="VN444_05890"
     CDS             complement(10075..>10319)
                     /locus_tag="VN444_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21376.1"
                     /translation="VGPALSGMGAMHSAEYFVETIIDPNASVAWRITHHKAEHKGYLG
                     PDGTSKMPSYNDSMTVQQLIDLVAYMKSLTAGEHRH"
BASE COUNT         2230 a         2969 c         2820 g         2300 t
ORIGIN      
        1 cagcgaaaaa gcaatccaaa gacaagaacg gccgctgaga tcttcacggg ctctgtgagc
       61 gcctctccga caaggtgccg gctccacagc ccgctcatcg tgacagccaa gcaccccatg
      121 accaggagga acgcctccaa ctcctcctcc acccaacgga cggagaacgg caataccagg
      181 accagcccca tgatcagccc caggccgatg attaccgtaa tctctgacat cgtcttctgc
      241 ctccaggctt gcggtcgcaa gcgaatgaaa taaaaagccg cctccgcttc tcgcggatgg
      301 cggcccgacc acccctgtat cctccggaaa taactggatg ctatccccgg acccccctgg
      361 aagactccat cacactgtta tgtcatatga agttacgttg ataatcagca acaaccccca
      421 acgctctacg aacgggaaac ggcgcgacca tgcagcctcg gtaggcgtca gtttcggatt
      481 tgctaccaga ttgcaccaac gctcactata gccaaaaatc gagcaaggtc aaggtaaatc
      541 gggctcaaat ttattggcag atctcggtga ccgcgtaaga ttggtggcgt ggcgttcgaa
      601 gtacgctccg ccgtgtcccg ggtctgccgg gcggcagtct tcggaaattc cctcgcatgc
      661 tcggtcactt tccgtgttcc ttcccggcag cggctcggtg ggtccccact ccttcggcga
      721 agtccgttcc ggctcctgcc atacccacac gatttccgga agagccattc ttaattcgcc
      781 agcgactcat gttaaaggtt cccaaatggt attcgccgcc ttcccattcc cactgcagct
      841 tcgtttttct gtcggcggtc ttcaagttgt ccgggatctg atccatatcc atattcatgt
      901 cccaggcttc gagtgacact gacggtgagg tgtcatcatc gccggaatcg tcctcttcca
      961 ccatacggat ctttaccgag tcgagaaagc cgataacgcc cagttgctcg ccccatatct
     1021 tggcgtcctc ccagtcgttg cagtcgaact ccacatatcc agaagcggga atcttggtcc
     1081 aaccctgacc atcgacgtta acctccatat ggatctcgtc agtgctatat tcatttccca
     1141 gggtcccgtt cgtctcatct ttgcagatga ggacgggaat accaggccct accatgcttc
     1201 caccgaacag tgttaggttg gtgcgccagc taacggcgag ggtatgcttc tgaagattgg
     1261 ccaccggccg tttgatacgc aggtataatg tctggttctg gttcgtctgg ctgttgtagg
     1321 ttgcaccgtt atcctgtacc agcggaccga tcagcgggtt tccgctgaga tcggctagag
     1381 gcgttacacc atcgtcctga acgaaatcga tcattggcgc ggcgagtgca cccgacagat
     1441 gccgactgga gaacttgaga gactgggcct gacccgaacc ggcctgatcc tcgatcctga
     1501 ctcggaacca ggcggcatct tcggcattga gcggtttccc gctgaggaag gcaaactcct
     1561 gcgggctatt gggcaagaga tcgcacgcat cgtccctggc ggtgcagttg aacttgtaga
     1621 aggcgacagc gtattttccc tgccacgtgc gctgcttgct gaatacacgc acatagtgtg
     1681 gaccggtggg caagacaaac ttttgcgcgt ctaccaggcc acactcgctc acaggcgcag
     1741 cggtggtcgc aggaccagga attctttcct gtaccggaac tccggtgccg caaacgacga
     1801 gcttggtgtt ttccagttgg taagcgccgg ccagcggtac ggacaagttg tctggggaat
     1861 agatctcgta gtgcaatagc ggggtatcgg ctgcattgat gccactgatg gcaatagcat
     1921 aggtgccagg tgcttccagg cgaaaccact gcatgctgcc gcgatgcttg agttcgtgat
     1981 gcagccatga tgtgcttttg agtcccttcc cgattgtccc aaggtctgca tcgcctactg
     2041 tctgtgcgag cctcgggttg cactggggcg caaacagatt gatgtccgcc gccaccgggc
     2101 ggtggttgct caacccctcc aattcctgag gaatccagat atgttgcacg caggtgacgg
     2161 atagatcggc ggggggccac ggtatttcgc cttcgttgtc gttcaagatc tgtcctgcgt
     2221 atgtgtcggc gatcggacgg ctgacgagga tgtagtcgag acgttcattt ttgttgtaac
     2281 gcgagtggcc ctgatcctgg ggtgaggtag tctccgccca gctatcgtat agcggtgtca
     2341 caccggcttg cgcaggcgcc ttgaaacgat ccacccattc ctgcgttgca tcggcaagga
     2401 aatcagcagg taaaccaagc gcctggctca cgccaccgag accgtctata ttgagatcgc
     2461 ccaatgccac caacttttcg gtatccgtcc acgaggcgaa cgtcttccca agcgtcgtct
     2521 ccacgatgtc ctgcatgtcg gccagttggt tcttacgcac ctccgggtgt gttgaatcgc
     2581 cactatcggc ctgggtgtgg gtgagcaaca cgtgaaggga gcgctttgac ggcaactcca
     2641 cctggaccag cgccacgccc ttggccgccc ggcaatctgc gaaggcgaag gcgcagtcgt
     2701 gaaattcctt gaaggcaagc cgcttcttga tccaccaagg cttaaagtct gtggcctcgt
     2761 aatcgttctt cacaaaaggt aatctttgga agaggaactt gctaaacaac atgaggccac
     2821 tgtcctcgaa attgccaccg aggaagcccc aatccttttt caactggatc atgtccggac
     2881 cggacttgct gatgtatttt acatagctgg gataaatccc gcccagcctt tcttccaaca
     2941 cgtccttcgt gtcctcatcc cacacctcat tgaaggcaac gatgtcatat ttctcgttgg
     3001 tcatcgccac tacggcaccg agctgctccg cacgcggttc aacattggca tcgcctaaat
     3061 ggcacaaggg gtcccacttg cacaaaaata tccgcgacag caggtaggta ttgtaggtaa
     3121 gaatgcgcac ggagtcgccg gtctctgcca actttcccgt tgacggttga tagctaagaa
     3181 cgcgccggga gttctcgata tcggccaaca ctttcgttgc cggttgaccg attgtgaatc
     3241 cacaaccgac agccacaacg cttagtagca gtgtaactgc agcgcatatt ctttttttat
     3301 ctatcatgct tcactatggt ccggctgcgt gttatgagaa tctatcaggg attgttacag
     3361 ccgatgatac attccccagt tgtggcgtgc aaatatcggg attaagtcag gatgaaagta
     3421 tggcgagtgt ctacacccat gccttgaatc gaggcggtcg gggaacctat agctcggctg
     3481 atcgcctcgg tccggccagg tgcaccccat cctagcgaca gcgggccagt gagctggaag
     3541 cctgatcaca ctgacagcac gcgaggcatg acggattgat gatccttacg attggcaggg
     3601 cgcacccctc caggcaggag gcaggctgcg tccgtgcttc cagtgtatcc cggatacgtc
     3661 gccgtttccg ggtagcgccg cacctgcatt ggattatgtt cggcctttcc tgccattctt
     3721 gcgcctcgca gcagctcgcg gaccgcagcc caataacccg gcacgatcgt tttcctgtat
     3781 accgggtcct cgggcagtcc ctccattaaa ccaggaagtc gatccaatag aggacgggct
     3841 taggcgtgag cagatcctgc acaagccttt tggcctcagg gcgcgaaaac ggtcgcaaac
     3901 ccgacgtcgc gggggctggc cattccggtc cctgcttggc ggcagaagac gccactttct
     3961 tatcgtcgta caatctccgg acgtcaccgc gaatgcgcgc gggggtacgt ccctaagaat
     4021 gtgttcacca agtgcacatt ccgtgtttat cggtagccct cgcgggatga gagcttccac
     4081 agcatgcctg aagcctctcc aatatcgttc acgctttgct ttacatcgac tgcgtttgaa
     4141 cggggatagg tatggatccc attgctctcc gatcgcgccg gccctgaggc tcctccatta
     4201 tcacgtggag caggttgccg ccatccattg aaggagcgcc atcacttcct tcacgtcccg
     4261 cacgtaatag tgggcctccg atccatcaag ggcgccgact cgtatcgtga tgccctcggc
     4321 gagtgcccga aaggcatctt catccgttcg atcgttaccg atgtaaatcg gtagagccga
     4381 gcgtcggcga ctgtgctgca tcacccacag cgcggcatgc ccttttgtcc ggttgagttg
     4441 tgggcggagt tcaatgacgt tcttaccgtg aaggactgtc agtcccgata agcgagcaac
     4501 gggaaggatg tcgcgtacaa atacccccac aagatgaggc gccagttcgt gaggtaccaa
     4561 ccggtaatgc aggctgatcg acagtccttt gtcctcgaca agcgcgccgg ggatctcggc
     4621 tacaagattg gtgagctgcg accgaatcct ggtgacggcc tcttgaaatg gcggtggcgc
     4681 gttcatcttg acctgccgcc catcgtgcca gatctccagc ccataatgac cgatgtagat
     4741 caggttacgt acccggacca gccgtcgcag atcatcgagc cgtcgtccgc tgatcacggc
     4801 gaccgtcaat cgaggatgac ccgcaagctc ctggagcact tcccgtgtcg atacgggaag
     4861 tgtcgcctgc tccgaacaag aggcgaacca cgttagtgtg ctgtcgtaat ccagaagcag
     4921 cacggtgtga ctactgctca ctatttccgc cgcaaccggc ggccactggg accagagcca
     4981 ttccatcggg gcgtctccgg tcaggtcatt gagacctgcg actcctcaac acgaacctgc
     5041 acgagttcac cgctgctcag aacttcctcc cggctcggcg ccagccttcg atcgcgactc
     5101 ggacgagctg actgcgtgtt cgtaccccgg tcttctgaaa caaatgctgc agcgtcgcct
     5161 tcactgctcc ctccgtcact ccgagttggg tagcggcttc cttattcgtg aggccctcaa
     5221 atacagcctg gagtacctgt tcctctcgtt cggtgaggcg cttatgatcc tcttgttcct
     5281 caggctgatt caccgcatcg gccatgagtt ggatgatcct ctgatcgacc catgcctccc
     5341 cacttacgac tcgccgaatg gctgtgacga gggcgtcagg cgggctgtgc ttgagaaaga
     5401 tacccgatgc gccgagcttc aatgccactg aggattccgc tgcgttcatc cccgcagtga
     5461 ccattaagat tttccctgtg tagcctcccc cgcgagccga taagataagc cgactcccgt
     5521 aatcctctcc gagatcaaag tccagcaata cgacatcaac gggtacacgg ctcaggatct
     5581 caagcgcctc ggctgaggta ccgcagaccc ctactatttc gaaatcgggc tccgattgca
     5641 gaagccggtt caagccctct cgaaatagga tgtgatcgtc aagcaacagc acccgtattc
     5701 gcttcatgtg tgcctgtact tccatcattc atctcagtgg aacgggctac tgccaagtca
     5761 atgacaaagg agcaccctgg taccgttgga tcgtaccgaa gatccccgcg aaacgatcgc
     5821 acgagcgctc gagagagaaa taagccgagg cccgttgatt cggcgcctgc ttgaaaaggt
     5881 cgaaacagct tttcggccga accgatccca ggtccggtgt cggtaacccg gatggacacg
     5941 ctgcctttcc cgacagagac ggagatagcg attcgcttca cgtcggcgcg ctccaaggct
     6001 cgttcggcgt tctttgtcag gttcagtagg gcctgcaata agaggtgatt atcggcccac
     6061 accggaggaa gtccttcagg gatctcccag tctactgtaa tgccggcccc ctcgcaggac
     6121 ggtcccagga cgatccgaag actgtcgaac agctcagcaa gatcggcccc gtcgatctgc
     6181 gtagtccttg ccccgcttct actgtgcttg aactccaatg aggcgatctt attcagtgtt
     6241 tcgaccagcg acccgagagc ctcgaaatcc ttatttgcca acaaacgccc gtcccgtgtg
     6301 aggttctcgt agagaacggc gatagcgctg cacacgtttc ggatttcatg tgagaccgca
     6361 ccaacaacga ctcgcgagcc ggccaagagg tgctcaagac tcgactcctc gcgttccctc
     6421 aattcttctg aagcatcgac gatcaaggca gccagtcgag gacctgccgc ggtcgtgtag
     6481 gttgagaaga agacgcgagc cgggaagatg ctaccgtctt ctcgcgcccc acggcattgc
     6541 atttcagtcc gaaaggtctg gcgtgtgttc tcaacagacg ggacagcacc caacgccgaa
     6601 acgtaacggc tgatatttct cccgggcagg tttccattcg gcgctccaaa caggcgatgc
     6661 gccgatgaat ttgcctgaag aattgcgcca tcgccggcca ttgtaaggat tgcaaccggg
     6721 ctgctgtcga tgagaaatgc taactgctcc tccgcttggc gccgagcaac tacttcctgt
     6781 tcaatccgct ggaggtgttc catctcccct cggcggctcc tgataacctc atggacgaac
     6841 aaccccgccc ctgtcaatgc ggcgaaaacc gagaggtcgc gtagcagccc gacgctggga
     6901 gtcaaaggaa aggggtcgaa gagatcggag agcaccgtac aaagaaccgc catcaaggca
     6961 atcgccccgc gcggcagcac actagctgcc agcatcacgg gaaatacata caggaatccg
     7021 aaagagacat cgatatccac gcgccaatcg atgacagcga cggttccaat gagaattgcc
     7081 gcacaggtaa gtacaatatg ccttctggca acgaacggag ggaaaatcat agcccagccc
     7141 acgtcggcgc acctccgcca tatcttagca tgcggttcct ccctgcccga aatcgctacg
     7201 ttcaacaggg ttcaagagat ccgtctgcct taggcgcggt tacggtcctg atgtgtccaa
     7261 atttgcgtca acctccgtcc ctttaggaag ggtccggata agcttgctgg agtacttcgg
     7321 ccttcactat tcatttagca cctccaacct tcgctcgcta gctatctttc ggctcagcga
     7381 aagctatctt tcggatgagt tggggcacaa gcgatgtgca gcggatgagt cgcttctctg
     7441 ggtgtgggga ccggagcctc tcggatggcg gactttgcgc ggcgtcggat gtcacgaggg
     7501 attctcgatg acgggaagta gcgtcctgct acagcgttcg cacgctgctc ggttcatctt
     7561 cgcagggagc ttcgagggcg gtcgccactg caacattaaa aaccactctc tgcgaaccgg
     7621 accgaatctc cttgtttatc gtatccctct gtgtcagcat gaatgaaata tctcccttcg
     7681 gtaaattgca gtagaggacg gagaggagaa aacactatga gagccaaaac gatcaacgca
     7741 gtccggggca tctccgtcgc tctccggtcg tcccggaggc cgtatggatc aatcaaccga
     7801 ggccggtgga acagagcgga gaagcgctat actaaattag gacaacagat gtcccaaagt
     7861 cattgacaga ttccgaacgg tcaagttgat caagttattt cgcgaaggct cataaagccg
     7921 gaaggggcat atagatatgg atgagagaga gatcttcatg gctaagcagg caatgcagtc
     7981 tgcactgatg aaagtccctg tgatgttagt gctgatgttt gtagcaattc tggtcggttt
     8041 tctgatttta catccatggg ttcagattgg tgcaggtgaa agaggggtcg tccttaattt
     8101 tggcgctgtt caggaggatg tgcttggaga aggactgcat ttcaggatac ctataatgca
     8161 acgaattatc ccaatggatg tcaaggttca gaaggcagtg actgatgccg catctgcatc
     8221 ggctgatctt caggatgtga ctttagcggt tgcgcttaat tatcatatcc tacctgataa
     8281 ggcaaatgtt gtctatcaaa cccttgggat ccagttcaag gagcgtatta tagaccctgc
     8341 aatacaagag gtaatgaagg ctgtgtcggc cagatacaca gcggaagagc ttatcacgaa
     8401 gaggccggcg gtaagcgagg caataagagc agctctttct gaaagactcc tggtgtataa
     8461 catagtagtg gatgcgtttt ctattgtcac attcagcttc tcgcgagtat ttactgaggc
     8521 tattgaggca aagcagacag cagagcagct tgcactaaag gcaaagagag acctggatag
     8581 gataaaggta gaggcagagc agacgattgc agcagccaag gcagaggcag agtccttgag
     8641 gctgcagaaa gcaaatattt caccggacct tatagagctt agaaagatag aggccaatct
     8701 cagggctata gaaaaatgga atggcatact tccccaggtt acaggcgctg gggctgtgcc
     8761 atttattggc gtaggtggga cgcaggagag caaggggaga aaggagaaag acggttagtc
     8821 gtcaggattg gtaactaact actacccatg aaacgaatac gacttcacat tgaccgtggc
     8881 agttccatat ctgcggctta tttctttaag gcttcttcag tactaattct gagctcagtc
     8941 gttcgatcga aaaacctcat cgcttgttcc tcgatggcga attcgtaact tcggccttgc
     9001 aggaaggaca ccgtgatgtc cgagggaagc ctggccaata catgggcatt tccgaactgc
     9061 tcccctagga gcctgtcgga gtattcacgg tgatactgtg tgcacgcagc aacattccgg
     9121 gtcctaagcg agcgcgggag ggcggtcggg taatccgcgt cccggggcgg cctatagccg
     9181 agaagattcc cgagtgcccc tcacgcaggc tgcaggcttt gcctcgcccg gaacagcttg
     9241 agcaggttgt gcgtgaggca gatcagcagc cactccgcgg tcaccttggc ctgtccccgg
     9301 aacgcaaagc gccggaagcc ccgcccggcg ccttgatctg gccgaatacg ggttcgacga
     9361 tcgccttacg cagcgcgtag atcgcctgtc cggcgacggt ccggagtttg tgccgcatct
     9421 gcgcgatgac cggcgcatcg ggcggcggcg ggtcgctgtc ggacggggac agctccccat
     9481 gtttctgccg gtccgggggc acgtacaggt cgatgccctg cagagtcatg tccgtcacat
     9541 gtggtgcgct gaagtatccc gcatccgccg agacctgcgt cggcttccgg tcgagattgg
     9601 cctccacgtg ctgcagcatc ggcacgagtt gcgctgtatc gttggcccgc tgggtgacgg
     9661 cggcggccac aatgatctgc gcctggccgt ctacgactgc ctgcgcgttg taggcctgga
     9721 cacaactctt cgtggcccca tccttcatga tccgagactc ggggtcggtg aagttgcgct
     9781 gggccgatag tgggccttcg acccctcaag ggcaccgact cgtatcgtga tgccatcggc
     9841 cagcgcctca aacgcatcct cgtctgttcg atcgtcaccg atgtaaatcg gtaagactga
     9901 gcgtcggcgg atgtgcttca tcacccacag cgcggcatgc ccttttgtcc aactgaaata
     9961 gtttttcgat catccgttca ccttcactct tctcgaaccc cccgtggctt tcccgctgaa
    10021 cctgtataag taaatgcttt tttcagcttg gcatctggaa cccagaacct cttgttaatg
    10081 cctatgctct ccagcggtca ggctcttcat gtaggccacc agatcgatga gttgctggac
    10141 cgtcatggag tcgttgtagc tgggcatctt tgatgtgcca tcagggccga gatacccttt
    10201 gtgttcggcc ttgtggtgtg tgatccgcca ggctacggag gcattcggat cgatgatcgt
    10261 ctcgacgaaa tactccgcgg agtgcatcgc ccccatcccc gagagggcag gccccacat
//
LOCUS       DATMXI010000306         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_255473, whole genome shotgun sequence.
ACCESSION   DATMXI010000306 DATMXI010000000
VERSION     DATMXI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255473"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..210)
                     /locus_tag="VN444_05895"
     tRNA            complement(124..210)
                     /locus_tag="VN444_05895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Leu,seq:cag)
     gene            293..691
                     /locus_tag="VN444_05900"
     CDS             293..691
                     /locus_tag="VN444_05900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWR21377.1"
                     /translation="MDYEDDHMCFVCGKQNGAGLHLEFELIGDDRIRTEFTPPKRFQG
                     WKDVLHGGIIATILDEVMVNCAYLRQIMAVTTKLQITLRRPAAIGERLIFYGQILKQG
                     TRTVNMKAWVEQEDGTIVAEATGLLMKIRG"
     gene            705..1532
                     /locus_tag="VN444_05905"
     CDS             705..1532
                     /locus_tag="VN444_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWR21378.1"
                     /translation="MRRVVTLLTDFGLSDPFVGIMKGVILSINPHAVIVDLCHDCKTY
                     DAAEAAFALITAYRYFPQGTIHVAVIDPGVGGPRRPILVTCDGHLFIGPDNGLLAPLA
                     EKAGSSGVRTIAATQYFLHPVSATFHGRDVFAPVAGHLSLGAKPAEIGEPIDDYVRLA
                     LPHAALSGTSLIKGEILHVDRFGNLVTNVTRTDLEHLASGDFPMAFFVRIAGQEVPVV
                     AYYGQVAPATPGAVIGSADYLEIFVNQGDASRLLGVGRGAEVMVGRKDASDRGESPT"
     gene            1690..2550
                     /locus_tag="VN444_05910"
     CDS             1690..2550
                     /locus_tag="VN444_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HWR21379.1"
                     /translation="MAKRSFADFYKGVSDDALGAYLKRIAQVPLLKKEEELLLGKAAQ
                     RGDEAALKQLVEANLRFVVKVALRYRGYGLSLSDLINEGNIGMLEAARRYSPEYNVKF
                     ITYAVWWIRQAIMQALAAGGGAVRLPLRKARLASQLNDIRADLSQQLQAEPTDSEVTK
                     ELDLSASALEGALHRAGQLAFFSDEIGLEQRIGRELYESKQLPPADYELIRRSFREEV
                     ERMLSGLTSRERLIVELRFGLGKEDAMTLKEVGKRLTLSRERIRQIEERAKQKLRVMA
                     KSRHLKDYLN"
     gene            2788..3447
                     /locus_tag="VN444_05915"
     CDS             2788..3447
                     /locus_tag="VN444_05915"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWR21380.1"
                     /translation="MADRYESAEAARWRMVEQQLRARDIVDERVLTAMGKVPRHLFIE
                     PAMECFAYEDRPLSIGAGQTISQPYIVALMIQLLRVQPSHRVLEVGTGSGYQAAILAE
                     LAAEVYTVEIIPSLAEGACAHLEALEYHNVHVRHGDGYEGWPEVAPFDGIVVAAGAPR
                     IPLPLIEQLREGGRMVIPIGIARETQDLILGEKRGGQFMVRSIAPVRFVPLTGKGGAE
                     Q"
     gene            3491..4168
                     /locus_tag="VN444_05920"
     CDS             3491..4168
                     /locus_tag="VN444_05920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWR21381.1"
                     /translation="MIPLRDNIPSSRTPVVTVGLIAANILVYVGQLMLPPHEAVQFIH
                     LYGLIPLEISGGDLLVPHRVPLYATVFTSMFVHGGLFHLGGNMLYLWIFGDNVEDRIG
                     RLKFLIFYLLSGLAAAAAQIWTDPVSKIPMVGASGAISGVLGAYLFLFPHARVLTLIP
                     LGFFYRLVEVPALIILGFWIIVQVLHGVGTLGVQVGGVAWFAHIGGFVAGLVMVVLLS
                     GGRKQLH"
     gene            4183..4578
                     /locus_tag="VN444_05925"
     CDS             4183..4578
                     /locus_tag="VN444_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWR21382.1"
                     /translation="MSAAKRRMALSRQEFRQLVSQAIASLPPLVMDRLTNVEVVVKDQ
                     PTHEELAVAGIDPTETLFGLYSGIPLTKRSSSYGMVLPDKITLYQRSIEEGCRTKQEI
                     QEQIRTTLLHEIGHHFGLSEDELEEAGYK"
     gene            4678..>6243
                     /locus_tag="VN444_05930"
     CDS             4678..>6243
                     /locus_tag="VN444_05930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWR21383.1"
                     /translation="MATRGRRREARFYELYCIVCGRTCTEQESSTRCVSCGKPLGVRY
                     DYTYIRARLNRYSLKTSPIKALKYLDFYPILNLDLVVSLDEGGTPLYRCHRLAEELGI
                     KQLYIKNEGLNPTGVFKDRGTLVEITKAKEQGAKAICVASTGNMAGSVAAYASIAGLP
                     CYVAVPEGTPIGKMAQALSYGARVLQIRGTYNDAASIAEQMSQRYKFYLAGDYAFRIE
                     GQKSQAFEIVEQLDWQAPSVVIVPMGCGTNIAALWKGFKEFHELGLISSLPRMIGVQP
                     VGCQPIVAAFNQGSDDIVPVKKPESVASALIAGDPLDGLKALAALRESGGCALSLSDG
                     EILQAQQQLARQESIFVEPSGALPVGALALLLTSGRVRADEAVVCLATGNGLKDPRAA
                     LRILPSPATIDPSMQEVEKFLKLRLYEIRAAGAKNGDKNLFEQAPSAAEVVTKVRQEF
                     GVKLTAEYGGKVRSLIEEFVKKGKPITKADLQYIVENVLKGLSVHKLVLTVEDFRVST
                     SLHGQAEAAVWVLF"
BASE COUNT         1265 a         1750 c         1912 g         1316 t
ORIGIN      
        1 gtgcaggaag gctacgttaa ggagaattct gcggccagac gcgagagatc gatatgttgc
       61 agagataagg agtaaaagac gaagaactct gctaatctga ggactgcaat tccgtaagtc
      121 tggtggtgcc gaagcgggga ctcgaacccc gatgagcgta cgcccactgc gccctgaacg
      181 cagcgcgtct accagttccg ccacttcggc acggtatgtc gtaagctgct tacgctcact
      241 acttgtagat ggtcaacgct gaaaagtcaa cggaaaagcg taggaagaac ctatggatta
      301 cgaagacgat catatgtgtt ttgtctgcgg caagcagaat ggagctgggc tgcacctcga
      361 attcgaactg atcggtgatg atcgcatccg gaccgagttt accccaccga agcggtttca
      421 gggctggaag gacgtcctac atgggggtat tattgcgacc attctcgatg aggtgatggt
      481 caactgcgcc tatttgcgac agatcatggc cgtgaccacc aaactccaga taacgcttcg
      541 acgccctgcc gccattggag agcggttgat tttctacggg cagatcctga agcagggcac
      601 cagaacagtc aacatgaagg cctgggtcga gcaggaggac gggacgatcg ttgccgaggc
      661 caccggcctc ctgatgaaga tccgaggata agaaggggga tcaggtgcgg cgggtcgtca
      721 cgttgctgac ggacttcggg ttgagcgacc cgttcgtcgg aataatgaaa ggggtgatcc
      781 tcagcatcaa cccccatgcg gtgatcgtcg atctgtgcca tgattgtaag acctacgacg
      841 ctgccgaggc ggcatttgct ctcatcactg cgtatcgcta cttccctcaa ggcacgatcc
      901 acgtcgcggt gattgaccca ggggtcggcg ggcctcgccg cccaatcctg gttacctgcg
      961 acggccatct gttcattggt ccggacaacg gactcctggc cccgctcgca gagaaggccg
     1021 gatcgtcggg cgtcaggacc atcgctgcta cccagtattt cctccatccg gtcagcgcca
     1081 cctttcacgg ccgcgacgtc tttgcgccgg tggcaggtca cctgtcgctc ggggccaagc
     1141 ctgccgagat tggagaaccg atcgatgact atgtccgtct cgcgctccca cacgcggcgc
     1201 tatccgggac ctcgttgatc aagggggaaa tcctccacgt tgatcggttc ggtaacctgg
     1261 tgacgaacgt tacccgcacc gacctggaac atttagcgtc cggggatttt cctatggcat
     1321 tcttcgttcg tattgccggc caggaggttc cggttgttgc ctactacggc caggtcgccc
     1381 ctgccacacc tggcgccgtc attggaagcg cggactattt ggagatcttt gtgaatcagg
     1441 gggatgcgtc caggctcctc ggcgtaggac gaggagccga ggttatggtc ggcaggaaag
     1501 atgccagcga tcggggtgaa tcccccactt gatcctaccc gaacgcctcc ttactggtgt
     1561 tgttctggtc ttccggccgc tcccctgtct ttgacaatct gtaaaacact gtgttaggct
     1621 ctagccacat agtagcccgg cttgttcatc acgcacatga ataccgactt aacgcacagg
     1681 tgagacacag tggcgaaaag gtcattcgcg gatttttaca aaggggtctc cgacgacgcg
     1741 ctgggtgcct atctgaagcg gattgctcaa gttccactcc tgaagaaaga ggaggagctt
     1801 ctcctgggaa aggcggctca acgtggggat gaggcagcct tgaagcaact ggtggaggcc
     1861 aatctccgtt tcgtggtcaa ggtagccctc cgctatcgcg ggtacggtct gtcgctatca
     1921 gacctgatta acgaaggcaa catcggcatg ctcgaagccg cgcgccgcta ctctccggag
     1981 tataacgtta agtttatcac ctatgcagtc tggtggatcc ggcaagcgat catgcaggcc
     2041 ttggccgcag gcggaggggc tgtgaggctt ccgctccgca aggctcgcct cgcatcgcaa
     2101 ctgaacgaca tccgcgccga tctctcccag cagttgcaag ctgaacctac cgactcagag
     2161 gtgacgaagg aattagacct cagtgcgagc gccctggagg gggcattgca ccgggctggc
     2221 caactggcgt ttttcagcga tgaaatcggc cttgaacaac ggattggaag ggagttatat
     2281 gaatcgaagc agctccctcc tgctgactac gaactgatcc ggaggtcgtt ccgtgaagag
     2341 gtggagcgga tgcttagcgg cctcacctcc agagagcggc tcattgtgga gttgcgcttc
     2401 gggttgggga aggaagatgc gatgaccctc aaagaggttg ggaagcgact cacgctctcg
     2461 cgggagcgga tccgacagat cgaggagcgt gccaagcaga aattgcgcgt catggccaaa
     2521 tccaggcatc tgaaagatta cttgaattag atcccctcag ctaccgagca attcaccatc
     2581 acgagaaggg tgggatattt cgcgtgaaaa tggattgcgg ttggatcgat aagccctgac
     2641 ctttcaccgg agatcggggc ttttgttttt acccagtatg gggcctaccc tctttacctg
     2701 gcagtcgcat gatgagaaat cgtgaagccc ggcatgggta cttcgccctt gcgtagctct
     2761 tcagtctgat cgtttcaggg ggatgcaatg gccgaccgat atgaaagcgc ggaggcggcg
     2821 cgatggcgga tggtagagca gcagctcagg gcccgagata tcgtcgatga gcgggtcctc
     2881 acggcgatgg ggaaggtccc acgtcacctg ttcatagagc cggccatgga atgtttcgcc
     2941 tacgaagacc gtcctctctc tatcggtgcc ggacagacta tttcccagcc ctatattgtt
     3001 gcgctcatga tccaactcct ccgggtgcaa ccgtcgcatc gggtccttga ggttgggacc
     3061 ggttcgggct atcaggccgc tatcctggct gaactggcgg ccgaggtcta taccgtcgag
     3121 atcatcccat cgctcgcgga aggagcctgc gcccacctgg aggctctcga ataccacaat
     3181 gtgcatgtcc gacatgggga tggatacgag ggatggcccg aggttgctcc ctttgacgga
     3241 atcgtggtcg ctgccggcgc tccgcggatc ccgctcccgc tgatcgagca gctacgcgag
     3301 ggagggcgaa tggtgatccc gatagggatt gcgagggaaa cgcaggatct gatcctcgga
     3361 gagaagcgtg gcgggcaatt catggtccgc tcgattgcac ccgtacgctt cgtgccgttg
     3421 actggaaagg gaggcgccga gcaatagctg aaagctgact gctgagggct gacagcttgc
     3481 gtaagcatgt atgatcccac tcagggataa tatcccctcc tctcgcaccc ccgtcgtcac
     3541 ggttggactc atcgccgcta acatccttgt ctacgtgggt cagttgatgc tgccaccgca
     3601 tgaggcggta cagtttatcc acctgtatgg tctcatccca ctggagatca gcggtggcga
     3661 tctactggta ccgcaccgcg ttccgctcta cgccaccgtc ttcacctcca tgttcgtaca
     3721 tgggggcctg ttccaccttg gcggcaacat gctctacctc tggatcttcg gcgataacgt
     3781 ggaagatagg attggacggc tcaagttcct gatcttttat ctgctctctg gactggcggc
     3841 tgccgctgcc caaatctgga ctgatcccgt ttcaaagatc ccaatggtcg gggccagcgg
     3901 agcgatctcc ggcgtgctgg gagcctatct cttccttttt ccccatgccc gcgtcctcac
     3961 ccttataccg ctcggttttt tctaccggct cgtcgaagtc ccggcgttga ttatcctggg
     4021 gttctggatc atcgtccagg tactgcacgg cgtgggaaca ctgggcgttc aggtgggggg
     4081 cgtcgcctgg ttcgctcata taggagggtt cgtcgcaggc ctcgttatgg tagtactgtt
     4141 gtccggcggt cgtaagcagc ttcactgaag ggatcggcta ttatgagcgc ggcgaagcga
     4201 cgaatggccc tgagccgcca ggagttccgt caactggtct ctcaggcgat cgcgtccctt
     4261 ccgccgcttg tcatggatcg cctcacgaac gtcgaggtcg tggtgaagga tcagcccacg
     4321 cacgaggagt tggcggtggc cggaatcgat cccacagaga ccctcttcgg cctctactcc
     4381 ggcatcccgc tgaccaagcg gagcagctcc tacggaatgg tcttacctga taagatcacc
     4441 ctgtaccagc gatcgattga ggaaggctgc cggaccaagc aagagattca agaacagatc
     4501 cgcacgaccc tgttgcacga gatcggtcac catttcggcc tctctgaaga cgaattggaa
     4561 gaggccgggt acaagtgatg ccacggttct cgcttctgga atactcttga cactttcggg
     4621 taacccccga taagatcgcg cctgttttgc gtctgtactg aggatcgagg aggatcgatg
     4681 gctacaaggg gacggcgtag ggaggccagg ttctacgagc tctactgcat cgtgtgcggc
     4741 aggacctgca ccgagcagga gagcagcacg cgttgcgtca gttgcggcaa gccgctcggc
     4801 gtacgctatg actataccta tatcagggct cgcctgaatc gatactcgct caagacctct
     4861 ccgatcaagg cgctcaaata cctcgatttc tatcccattc ttaacctcga tctggtggtc
     4921 tctcttgatg agggcgggac gccgctgtac cggtgccatc ggctggcgga agagttgggg
     4981 atcaaacagc tctacatcaa gaacgaaggg ttgaatccga ccggcgtttt caaggataga
     5041 ggaacgctcg tggagatcac caaggccaaa gagcaaggcg ccaaggcgat ctgcgtggcg
     5101 tccaccggga atatggccgg ttccgtcgct gcctacgcct cgatcgccgg gctgccgtgc
     5161 tatgtggcgg tgccggaggg aaccccgatc ggaaagatgg cccaggccct ctcttatgga
     5221 gcccgggtcc tccagattcg agggacgtac aacgacgcgg cctccatcgc cgagcagatg
     5281 agccagcgct acaagtttta cctggcaggc gattacgcct tccgcatcga aggccagaag
     5341 tcgcaggcgt tcgaaattgt ggagcaattg gactggcagg cccccagcgt cgtcattgtc
     5401 cccatgggtt gcggcacgaa catcgccgcc ctctggaaag ggttcaaaga gtttcatgaa
     5461 ctgggcttga tctcgtctct gccgcggatg atcggcgtcc agccggtcgg ctgtcagccg
     5521 atcgtggccg cgtttaatca ggggagcgat gacattgtgc cggtaaaaaa accggagtca
     5581 gttgcctcgg cgctgatcgc aggcgatcca ctggacgggc tgaaggcgct ggcggcactc
     5641 cgcgaatcgg gtgggtgtgc cctgtcgctc agcgatggcg aaatcctgca ggcccagcag
     5701 cagctcgctc gccaggagtc gattttcgtg gaaccgtccg gggcgctccc ggtcggggcg
     5761 ctcgcgctgc tgctgaccag cggacgcgtg agggcggacg aggcagtggt ctgtctcgcg
     5821 acaggcaacg ggctcaagga tcccagggca gcactgcgaa ttctcccgtc cccggccact
     5881 atcgatccct ccatgcagga ggtcgagaag ttcctgaagc tgcggctcta cgagatccgg
     5941 gccgccgggg ctaagaacgg cgataagaac ctgtttgagc aagccccgtc ggcggccgag
     6001 gtcgtcacga aggtgcggca ggagttcggg gtgaagctca ctgcggagta cgggggcaag
     6061 gtgcggtctt tgatcgagga gtttgtgaag aaggggaagc cgatcaccaa ggcggacctt
     6121 cagtatatcg tggagaatgt tcttaagggg ctttcagtcc acaagctcgt cctcacggta
     6181 gaggatttca gggtctcgac gtcgctccac ggccaggcgg aggcggccgt ttgggtcctg
     6241 ttc
//
LOCUS       DATMXI010000307         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_227820, whole genome shotgun sequence.
ACCESSION   DATMXI010000307 DATMXI010000000
VERSION     DATMXI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227820"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VN444_05935"
     CDS             <1..747
                     /locus_tag="VN444_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWR21384.1"
                     /translation="SVILGQVLRKAATAATGSLVLNAVRETKRLVGLNTNLGIALFFA
                     PLAKAALRRGHCPLRSRLRSILTSLTPVDGQRVYEAIRLAAPGGLGDADQFDVGATHG
                     RIPLLDAMRVAADRDSIAREYVTDFEITFTIGAPSLERFLQESGDPGPAIIQTYLTLL
                     SRIPDSLIARKCGAHEADRISREAGKILAVGGAFTQEGRRRLKHLDRTLRRDGNRLNP
                     GTTADLTASALFVVLLEHGIQCVLGRGNRL"
     gene            complement(742..1770)
                     /locus_tag="VN444_05940"
     CDS             complement(742..1770)
                     /locus_tag="VN444_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HWR21385.1"
                     /translation="MGVTVKAHARLHFGFIDPDGSLGRRFGSIGLAIDEPAVVLEAAP
                     ADRLEAVGRERGRVLTLARRFLRHYNIRQGVRIAVKTTIPAHTGLGSGTQLALSVASA
                     LARLFSIDADVRELAGVMGRGGRSGIGIAAFERGGFIVDAGHRVSATSEPVSGFGFRV
                     SGGLKQTETRRLDLVPPTIARYPFPKDWTFVVAIPRSGRGLAGVKEVRAFGRLAGRPA
                     AAAGRLSRILLMQMLPAVLERDPVVFGESLTMIQRIVGGWFRSVQGGIFATTQGAELA
                     RVMRRAGALGVGQSSWGPAVYGLAKDQEMAKSIMEKMSKVVPAKIHADIFPAQASNRG
                     AEISLSSL"
     gene            complement(1761..2318)
                     /locus_tag="VN444_05945"
     CDS             complement(1761..2318)
                     /locus_tag="VN444_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWR21386.1"
                     /translation="MIIESIVTTLNESGEANFAPMGVTIGKEEILIRPYTDSATCRNL
                     VATGVAVVNLTDNCRLFAESAIASPQFPHFPAELVTGLVLKDACSYYECSVMHADTAA
                     PRATFRCKIVKKGVLREFIGFNRAKNAIIEAAILATRVRLLEMETILQEYRRLSEIVR
                     KTGGEQEAQAMRYLQDYVERQPRWG"
     gene            complement(2435..>2583)
                     /locus_tag="VN444_05950"
     CDS             complement(2435..>2583)
                     /locus_tag="VN444_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWR21387.1"
                     /translation="HLGPEPIGHAFYLGRELTKARLAMLLGKKYIQEDDLRWGYLEHG
                     LPGD"
BASE COUNT          510 a          799 c          768 g          506 t
ORIGIN      
        1 tccgtcatcc tgggacaggt cctgcggaag gcggccaccg ctgctaccgg ctcgctggtc
       61 ctcaacgcgg ttcgagagac caagcggttg gtcggcctga acaccaacct tgggatcgcg
      121 ctcttctttg ctccgttggc aaaggcggcc ctgcgtagag gacactgccc gcttcggtca
      181 cgccttcgca gcatactgac ttccctgaca ccggtcgacg ggcaacgggt ctatgaggcg
      241 attcgcctgg cagcacccgg cgggctcggg gacgcggacc agttcgatgt cggcgcgaca
      301 catggccgga ttccgctcct tgacgcgatg cgcgtcgcag ccgatcggga ctcaattgcg
      361 cgcgagtatg tcaccgactt cgagatcacc ttcaccatcg gcgcgccgtc gctcgaacgg
      421 tttctccagg agtccggaga tccggggccc gcaatcattc agacttatct cacgctgttg
      481 tctcggatcc cggattccct gatcgcgcgc aaatgcgggg cgcacgaggc ggaccggatc
      541 tcacgggaag cgggaaagat cctggctgtc ggtggggcgt tcacgcagga ggggcgacgg
      601 aggcttaagc atttggaccg aactctgcgg cgagacggca accgtctcaa tcctggaacg
      661 accgccgatc tgaccgcctc ggctcttttc gtggtcttgt tggaacacgg gatacagtgt
      721 gtgctgggca gaggaaatag gctatagaga ggaaaggctg atttccgctc cgcggttgga
      781 ggcttgtgcc ggaaagatgt cagcgtgaat ctttgcagga acgaccttgc tcatcttttc
      841 cataatcgat ttggccatct cctgatcctt tgccaggcca tagactgccg gtccccatga
      901 gctttgccct actcctaacg cccccgccct tcgcatcacc ctggccagct ccgccccctg
      961 tgtggtggca aagattccgc cctgcacaga tcggaaccag cctccgacaa ttcgctgaat
     1021 catcgtgagc gattcgccga agacgacggg atcgcgctcg aggacggccg ggagcatctg
     1081 cataagcagg attcgactga gccgtcccgc ggcagcggcg ggacgaccgg cgagccggcc
     1141 aaacgcgcgc acctctttca ctccagcaag cccgcggccg gagcggggaa tggccacgac
     1201 aaaggtccag tctttcggaa aggggtaacg agcgattgtc ggcggaacga gatccaagcg
     1261 tcgagtttcg gtttgcttca acccacccga aacccgaaac ccgaaacccg aaactggttc
     1321 gctggtggcg ctcacccggt gtccggcgtc tacgatgaag ccgcctcgct cgaaggcggc
     1381 gatgccgatc cctgaccgac ctcctctgcc cattacgcct gccaactccc gaacatcagc
     1441 gtcgatagag aacaggcgag ccagcgcaga agccacgctg agtgcaagct gcgttcctga
     1501 gcccaatccg gtatgggccg ggatcgtcgt cttgacggcg atgcgtaccc cttggcgaat
     1561 gttgtaatga cggaggaacc ggcgcgcgag cgtaaggact cgaccccgct ctcgcccaac
     1621 ggcttcaagg cggtctgccg gcgcagcctc gaggacgact gcgggttcgt cgatggccag
     1681 cccgatactc ccaaaccgtc gccccaatga gccgtccggg tcgatgaatc cgaagtgaag
     1741 cctggcatgg gccttgaccg tcacccccat cgaggctgcc gctccacgta gtcctgaagg
     1801 tatcgcattg cctgcgcttc ctgttctcca ccggtcttcc gcacaatctc ggacagacgg
     1861 cgatactcct gaaggatcgt ctccatttcc aggagtcgaa ccctggtagc caggattgcc
     1921 gcctcaatga tggcgttctt cgctcggtta aacccgataa attcccgcag cacccctttc
     1981 ttaacgatct tgcagcggaa ggttgcgcgc ggagcagcgg tatcggcgtg cattactgag
     2041 cactcataat acgagcaggc gtctttgagg acaagaccgg tgaccagctc ggccggaaag
     2101 tgtggaaact gagggctggc gatggcgctt tcggcgaaca gccggcagtt gtcggttagg
     2161 ttgacgacag cgacaccggt tgcgacaaga ttccgacagg tagccgaatc ggtatagggg
     2221 cggatgagga tctcctcttt cccgatagtg acccccatcg gcgcgaagtt cgcttcccca
     2281 gactcattca gtgtcgtaac gatgctctca ataatcatag ccctataatt cagcgttcag
     2341 cgttcagcgg tcagcaatca gccgtcagca taaaaggtga tacggccgct gaaagctgag
     2401 agctgaccgc tgatggctaa tagccgctac tcggtcaatc cccggggagg ccatgttcaa
     2461 gatagcccca tctgaggtcg tcttcctgga tatatttttt tccgagcagc attgcgagcc
     2521 gtgccttcgt taactcacga cccaggtaaa aggcgtggcc gatcggttcc gggccgaggt
     2581 gcg
//
LOCUS       DATMXI010000308         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_245860, whole genome shotgun sequence.
ACCESSION   DATMXI010000308 DATMXI010000000
VERSION     DATMXI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245860"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VN444_05955"
     CDS             complement(<1..1303)
                     /locus_tag="VN444_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWR21388.1"
                     /translation="MQWWRGRRTSKLAEQRSLRVLFFIVGMLFLLVTIWAVWDEGKTR
                     RPWKAYQQEFNRLEHERVARELTTERSKSGAPEVRTALDKLHEDLKAAQARLAGAESA
                     QAQQTLAQRETEYTEMNMKAQFIKSELDEALYWVEHAFHNKQDSTKPRAKVAAIEKQL
                     RGLTAQVNILNARVEEARDVVKRFQVDVDTIRGRIDELASPVITLEKRLESIGARPLE
                     IKQIVVEGLAINEFKIPILTVDRCATCHLAIDRPGFDHVKQPYRTHPYREVLFGNHPI
                     ARFGCTSCHQGQGPTLDVEAAHGEVSHWARPLLRGDFAQTSCRKCHAEKKEFALAPVY
                     SRGRQMVEDLGCFGCHTMAGFEKIQKVGPDLTRVASKVDPNWLVRWIKKPREYLPKTK
                     MPHFGLSEDDALSIAAYLLRASQPVPELQSAFKAPASVERGR"
     gene            complement(1520..2329)
                     /locus_tag="VN444_05960"
     CDS             complement(1520..2329)
                     /locus_tag="VN444_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HWR21389.1"
                     /translation="MAEAKQNTEAKPAVQSAGTNEPAVADRVHVWPYLVRNEFIASII
                     VMIILTVWSITIDAPLEEAANPTQTPNPSKAPWYFLGLQELLVYFDPWFAGVVLPSLI
                     IVGLMVIPYVDINPKGNGYYTFRERPFAIATFLFGFLFLWVSLIITGVFFRGPGWNLF
                     WPWETWDPHLVVAITNVDLSTWGPFKWMGNPKIFGVEIIGFVLIVLYFSTAVVFYYLR
                     RDMSDAIRQLGPVRYGIVAFLFLTMMSLPIKIILRLAFNIKNVLVTPWFNI"
     gene            complement(2389..>2980)
                     /locus_tag="VN444_05965"
     CDS             complement(2389..>2980)
                     /locus_tag="VN444_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWR21390.1"
                     /translation="CMGGITFLVFLVTVVTGVILMFYYRPVAEYAYADMKYLEHDVPF
                     GMIMRNMHRWAAHGMVITIWLHMFRVFMTGSYKPPREFNWVVGVVLLTITLLLSFTGY
                     LLPWDQLSIWAVTVGTNMARATPLLGSEGPFGEVVGVTPRYDARSFLLGGTLVGPSAL
                     LRFYVLHCILLPILASLLMIVHFWRIRKDGGVSGPL"
BASE COUNT          705 a          875 c          780 g          620 t
ORIGIN      
        1 gccttccacg ttcgactgaa gcgggagcct taaacgcact ctgaagctcc ggcacaggct
       61 gagaagcacg gagaagatac gccgcgatac tcaatgcgtc atcctcagac agcccaaaat
      121 ggggcatctt cgtcttaggg agatactctc ttggcttttt gatccaccga acgagccagt
      181 tcggatcgac cttgcttgct acccgtgtca gatccggtcc cactttctga atcttctcga
      241 accccgccat cgtgtgacag ccgaagcagc cgagatcctc taccatctgt ctgccgcgcg
      301 aatagaccgg cgccagcgca aactccttct tttcggcatg gcactttcgg caactcgtct
      361 gcgcgaagtc gccacgcagc agcgggcgcg cccagtgaga gacctcgccg tgcgccgctt
      421 caacgtcgag ggtcggcccc tgtccctgat ggcaagatgt gcaaccgaaa cgggcgatcg
      481 gatgattgcc aaacaggacc tcccgatacg gatgggtgcg gtagggctgc ttgacatggt
      541 caaacccagg ccgatcgatc gcaaggtggc atgtcgcgca gcgatcgacg gtcaggatcg
      601 gtatcttgaa ctcgttgatc gccaggccct ccacgacaat ctgtttgatc tcaagggggc
      661 gagccccgat tgactccagc cgtttctcaa gggtgataac cggagaggcc aactcgtcaa
      721 tcctgccacg aatagtatcc acatcaacct gaaatcgctt gacaacatcc cgagcttcct
      781 ctacccgtgc gttcagtata ttgacctgcg cggtgagtcc tcgcaactgc ttttcaatcg
      841 ccgccacctt cgctcggggc tttgtgctgt cctgtttgtt atgaaaggca tgctccaccc
      901 agtagagcgc ttcatccagc tcgctcttga taaactgggc cttcatgttc atctctgtat
      961 actcggtctc acgttgagca agagtttgtt gtgcttgagc ggactctgct ccggccaacc
     1021 tcgcctgcgc cgctttcagg tcctcgtgca acttgtcgag tgcggtccga acctccggcg
     1081 caccggattt cgatcgttct gtagtgagtt cacgagcgac ccgttcatgc tccagccgat
     1141 tgaactcttg ctggtatgct ttccacgggc gtctggtctt cccctcgtcc cacactgccc
     1201 atatagtcac caggagaaag agcatgccca caataaagaa caagacccgc aaggatcgct
     1261 gctctgcaag cttactcgtc cgccgccctc tccaccactg caccaggcaa cctcaactga
     1321 actgtaacgt caaatggtaa cgactcaggt gtttagactg aaggctgaag gctgttagtc
     1381 gttgcgcatg gaccgactca aggcgcccag aaccttttcg gtctccatca tgttcacgtc
     1441 gaatccagaa aacgcctggc tcatctgtgc gcgttcctat agtctaacag cctacagtct
     1501 atctacctaa gtagtgacgt caaatattga accatggagt caccaagaca ttcttgatgt
     1561 tgaatgccag gcggaggata atcttgatcg gaagactcat catcgtcaga aagaggaagg
     1621 ccacgatccc gtagcgcaca ggacccaact gacggatagc gtcgctcatg tcccgcctga
     1681 gatagtaaaa cacgaccgcc gtgctgaagt agagaacgat cagcacaaac ccgattatct
     1741 caaccccaaa gatcttgggg ttgcccatcc acttgaacgg accccaagtg gaaagatcga
     1801 catttgtgat cgcaacgacc aggtggggat cccaggtctc ccacggccag aagagattcc
     1861 acccggggcc cctgaagaaa acgccagtaa tgatcagcga gacccacagg aataaaaagc
     1921 cgaagagaaa cgtcgcgatg gcgaagggcc gctctctgaa ggtgtaatac ccgttgccct
     1981 tgggattgat atcgacatac gggatgacca tgagcccaac gatgatcaag ctcggcagga
     2041 cgacgccggc aaaccaagga tcgaaataga ccagcagttc ctgaagccca aggaaatacc
     2101 acggagcctt acttggattg ggggtctgtg tgggattggc tgcctcctcc agaggggcat
     2161 cgatcgtgat cgaccacacg gtcagaataa tcatcacaat gatcgaggcg atgaactcgt
     2221 tgcggacaag gtagggccag acgtggactc gatcggccac tgcgggttca ttcgtgccgg
     2281 cagattgtac agccggtttc gcctctgtat tctgttttgc ttcagccatc gcttcgctcg
     2341 tcgctgatcg ctagttgtta ctcttcactc atcattagcc actcatcact ataacggacc
     2401 cgagactccc ccatctttcc tgatcctcca gaaatgtacg atcatgagga ggctggccag
     2461 gatgggtaac aagatacaat gcaggacgta aaacctgagg agcgcagaag gtccaacgag
     2521 ggtaccacca agcaagaagg acctggcgtc ataccgcggg gtgacgccga ccacctcgcc
     2581 aaatgggccc tcactcccaa gaagtggggt ggcgcgggcc atgttggttc cgaccgttac
     2641 ggcccagatc gaaagctggt cccatggcag gaggtagccg gtaaaactca ggagtagcgt
     2701 gatggtgagc agcacgaccc ctacaaccca gttgaactcc ctcggcggtt tgtaggagcc
     2761 ggtcatgaag acgcggaaca tgtgcagcca gatcgtaatc accatgccat gggccgccca
     2821 ccgatgcatg ttgcgcataa tcataccaaa cgggacatcg tgctccaggt atttcatgtc
     2881 ggcataagcg tactcagcta ccgggcgata gtagaacatc aggatgacgc ctgtcacgac
     2941 cgtcaccagg aacaccagga aggtgatgcc acccatgcac
//
LOCUS       DATMXI010000309        10789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_12869, whole genome shotgun sequence.
ACCESSION   DATMXI010000309 DATMXI010000000
VERSION     DATMXI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12869"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VN444_05970"
     CDS             complement(<1..1067)
                     /locus_tag="VN444_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWR21391.1"
                     /translation="MSTFSTAITGLKAYQTDLGVIGNNIANANTTGYKASRAQFSDLL
                     VQTLEGASAPSATTGGTDPLQIGAGVIVGGVQNIQTQGAIEPTGRSSDLAIQGEGFYV
                     LSNGENQIYTRVGTFELDANGNLVDSGTGFKVQGINGDISVPLGSNAAAGTTEVELAG
                     NLDASAANDPITPTDESALETSFFIIDSLGGKHQVNVTFTLPGSANTGTWNYAVSSPD
                     GAFTGASAGTITFASGGSIDASLTTGPASAGGVFTFNPGNAAASGQEFSIDLGDLTGF
                     AALSNVSMKRQDGVPPGSLSSFNVGSDGKVNGIFSNGLVKEIDTLKLASFSNPAGLLR
                     LANGQFAETPNSGVAQFGAAGT"
     gene            complement(1150..1848)
                     /locus_tag="VN444_05975"
     CDS             complement(1150..1848)
                     /locus_tag="VN444_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWR21392.1"
                     /translation="MEITSGSATTVQSPQGGGVATGSPLDRGLGKDAFLKLLVAQLRN
                     QNPLNPLQGTEFIAQTAQFSSLEQLQQINTSLAKLSTSSAGTDRASLDAVLASSYIGK
                     VVAANGTIIELGENNPAPLRYSLPAAATVAVQVRDLQGNTVRTIPLGAQVAGRYELTF
                     DGLGADGYPLPPGRYLYKVVATDAAGVGVAGAGTASGVVTSIHFEETQPFLIVDGSMV
                     PLSAISQMSLASQG"
     gene            complement(1906..2649)
                     /locus_tag="VN444_05980"
     CDS             complement(1906..2649)
                     /locus_tag="VN444_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HWR21393.1"
                     /translation="MIPVLAAVGIKVLSALAGKTITALMEQGLDSSTAVDRTERSFDT
                     ILERSRSMRSTRSDARPVGPLSSQGLAPTVISGSAADASVGLLTARLATRRTFAPATA
                     LPTVPQGPVSARAAADLIGRKIAANGSAIELRGSVPSTLLYRLPTAAESVQIEVRDLQ
                     GTIVRTVQLGPQPGGFHQMMFDGRGLQSGLYVYRVIATDTAGQPMARVSTAFGRVMGV
                     QFENGQPFLNVGSALVPLTGIFEVSSDQR"
     gene            complement(2701..4914)
                     /locus_tag="VN444_05985"
     CDS             complement(2701..4914)
                     /locus_tag="VN444_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWR21394.1"
                     /translation="MIPLQVADAAVFSAPQGIAAETMCPQGAADFLSLFEQMIGSGVA
                     AQVADETVPGGPIGALDVLTGEKRLPACRSSSDGSSEKGQAERNIDPAAAILVAIFSG
                     STQSAVLEPSAGCDYIADLLPSDMAAAPAAVTFLEVSTQPLFTREAGSMLESADIHAV
                     DSVGVAISVDSTRLLSVEIDPHLKPAVSSQETGATLVTSGPSLRPQGLSIMTLPADAE
                     THTAYGDGLLMSMQVRTTLPPFSLQVSSWRGEGQSEGDLRGHDPHLAEQPTEPARGVQ
                     EVVRIERFEARSDVDRVADLTAPAPAVVLSYAPSTEGTGLTPPRPPGAPAATTTSRRS
                     VFLEGDAATYPKTDISEESTIVSAIPEMVSPSPDTGLSAREVWTEPSGTVQQPPAVPQ
                     GSSQTSKPDAARPTLVMPLVNNERSAIGNQQSAGGGVNIPATSAKEVQDGINRLNDAQ
                     APGQVGGLLPIATLLGDSAKVTVALSEEAAGRSSFPQRPAVRITISSGVSEALGSEAR
                     DSEGFRDQTQVNADDDRAALRQESPRIETTQWLKQDESASDSESGSSAPDRSSSPSSD
                     RPGLHADCTLSAASRTEGLRSGPVVPFWHPRTLSDQLAEPIVMAARLSQRNEGKHVQL
                     RLHPQTLGELVIEVSWKDSGIVAAIKVQHHVAGELLTNDLGRLRIALVEQGIPISDLG
                     VQVGLDLHQWSYAGNGSQDSHAIGYLPEVMLQHDQGSIPPMIPVMGSDSLIDITV"
     gene            complement(4911..5675)
                     /locus_tag="VN444_05990"
     CDS             complement(4911..5675)
                     /locus_tag="VN444_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21395.1"
                     /translation="MKSRIALYAGYFVAVLLLASYLTGVLQQGILPRFGRPAAAPQQD
                     EQPAISDQPSTVQESDQAHDTGSAPADQASLQPPEEKPLAQPALQKSGASASHLPQQT
                     AAATGTPPSPSGEQRPVISDQEKALAEKRAELLRLEEQIRKRKEENQIEEKWLAELKA
                     TGAKLTKERDARREAGVKRLATLYEGMDPEAAASILSKLKREMATEVLAAMKARQASK
                     VLAAMNGQKAKELSERLEDAGVDRTINQKKAGEATP"
     gene            complement(5672..6136)
                     /locus_tag="VN444_05995"
     CDS             complement(5672..6136)
                     /locus_tag="VN444_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis chaperone"
                     /protein_id="HWR21396.1"
                     /translation="MKRFRFSLQSVLAVREAKADQAEAALAERQRACGALRERLAKLR
                     DAEAEALAELAVGQNIAQPESSSLAVRCAYLLGVGKQIRALEAMLAAEQAETVRLRRM
                     LLERSRDEQIVEHLKKRKIRVFRSEIAREQQAEIDEVAGGRRAANAFRGSVS"
     gene            complement(6152..7591)
                     /locus_tag="VN444_06000"
     CDS             complement(6152..7591)
                     /locus_tag="VN444_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HWR21397.1"
                     /translation="MSDARFDWGRARARVDKANLIRSLGKVTKVVGLVIEAVGQEAFI
                     GELCRIRSAGRHDVWAEVVGFHGEILLLMPLGEIHGIRPGSEVVAAGRPFEVPVGRSM
                     LGRVINGLGRPIDGKAPVEVEARCSVHRHPPSPMTRAPIIEPLATGIRVIDGLLTCGK
                     GQRVGIFAGSGVGKSTLLGMIARYTEADVNVIALVGERGREVREFITKELGEDGLRRS
                     VVVVATSNEPSLLRRQAAYVATAIAEYFRDQDKHVLLMMDSLTRFALAQREIGLSVGE
                     PPTTRGYPPSAFAMLPGLLERAGTADHGGSITGLYTILVEGDDMNEPVADHARAILDG
                     HIVLTRELAERNHYPPVDFLGSVSRLMSVVAPREVRVAASKLRELLAAHRQAQDLINI
                     GAYVSGSNPKVDEALRYMTPIEAFLRQSSDERSDFPGMVERLVTLFAPETGAEAPPRR
                     KKPDPRVEASYLAAASMARTGVAATQAVA"
     gene            complement(7588..8277)
                     /locus_tag="VN444_06005"
     CDS             complement(7588..8277)
                     /locus_tag="VN444_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HWR21398.1"
                     /translation="MSKIVTAGVGGWERASFEELVYSAGRSAGGAWATEHEPAQSGYE
                     SIRAAEREAEAIILSAREAAQQIEREAEREGRAKGRQDAIDEMRRRFEPLETLLQETC
                     RQVASARQAVITNAEEELVRLAVAVAERVLRSELGARRDAAVQFARVALEVAGSRKIM
                     AIRVNPGDYELLMEHRGELLGSPESARIIPDPAISSWGCVVEVESGLVDARVESQLHE
                     AARLLGRDEIS"
     gene            complement(8270..9286)
                     /gene="fliG"
                     /locus_tag="VN444_06010"
     CDS             complement(8270..9286)
                     /gene="fliG"
                     /locus_tag="VN444_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWR21399.1"
                     /translation="MTSHETLTGPQKAAVLLLTLGVESAAQIFKQLEDEEIEAITGEI
                     TRMKGVSAKASIAVIEEFQQMARAQEFMSIGGIRYAQDVLEKAVGRNKASAIIERLQG
                     LKGASFFSALKKVDPRFLLDSVRQEHPQTIAVVLSHLEPTTSASVLAGLPQAVRADVV
                     MRIANMDKTSPEVIGEIEHILDHRLSSVVNQEVSISGGVKQVAEILNSMDRNSERGVF
                     QVIQENDPYMADEIRKLMFTFDDVVLVDDRGIQRVLKEVEQKELALAMKAAGPEVAEK
                     LFKNMSERAGGLLKEEIEYLGPVKLRDVEASQQKIVAIVRRLEEAGEIIVQGRGGGAE
                     EIIV"
     gene            complement(9283..>10789)
                     /gene="fliF"
                     /locus_tag="VN444_06015"
     CDS             complement(9283..>10789)
                     /gene="fliF"
                     /locus_tag="VN444_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWR21400.1"
                     /translation="LWIAFSTHRPDMTTLFSQLNSSDAGAIVDELKSSKVSYRVVDGG
                     TRILVPSAVVHEIRLHLATRGLPQGGGVGFEIFDRTTLGTTDFVQRLNYQRALQGELA
                     RTIGQLKEVTAARVHLVLPQPSVFTEQEKPATASVVLNLRPGARLTPEQVRGIVHLVS
                     SSVEGLNADRVTVVDTSGKLIARPAEGRLGSGSTGQFEVQEAVEVELERRVRTMLEEI
                     LGPNKATVRVAAQMDFTSVERTEERFDPRGVIKSEQRTTETQQSSTATPTAVAGVASN
                     VPDQAATQSQSGKSTAKSTKEAETVQYDVSKVLERKVFSPGELKRVSVAVMVDGTYKA
                     VPDGKGGERKEYVPRKSDELEKIKVAVKNAVGFSAARGDEIEVVEFPFDTSAMEKERA
                     LMEEAERKDFWYSLVKPILMVVGVLLVLVFGLRPLIRSLKERRLPTFDAPGMLSMSQP
                     SPTAIETGPDQAPALDVASDDPLRAGLMELARNNPNVVAQLVRAWMVKRPS"
BASE COUNT         2063 a         3550 c         2983 g         2193 t
ORIGIN      
        1 gtgccggcag cgccgaattg ggccaccccg gagttcgggg tctccgcaaa ctgtccattg
       61 gcgagcctca gcagaccggc cgggttcgag aagctggcga gcttcagcgt atcgatctcc
      121 ttcactagcc cattactgaa gatcccattg accttaccgt cacttccgac attgaaggag
      181 ctaagactgc ctggcggcac tccatcctgc cgcttcatgg aaacattcga caaggccgca
      241 aatccggtga gatcgcctaa gtcgatgcta aactcctgac cggaagccgc cgcgttcccc
      301 gggttaaagg taaagacacc tcccgcggag gctggtcccg tggtgaggga ggcatcgata
      361 ctaccgccgc tggcaaaggt gatggtccct gccgaggcac cggtgaaggc gccgtccgga
      421 ctcgacacgg catagttcca tgtacccgta ttggccgacc ccggcagcgt gaatgtgacg
      481 ttcacctggt gcttgcctcc cagagagtcg ataatgaaga agctcgtctc aagggcggat
      541 tcgtccgttg gtgttatagg atcattcgcg gcactcgcat cgaggttacc cgccaattcg
      601 acctccgtgg tccccgcggc ggcattgctc cccaacggga cagagatgtc gccattgatc
      661 ccctgcacct taaagccggt cccggagtcg accagattac cgttggcatc gagctcgaag
      721 gtgcccaccc gcgtgtagat ctgattttca ccgttgctca ggacgtagaa gccctcaccc
      781 tggatggcga gatcggacga tcgaccggtc ggctcgatcg ccccctgtgt ctgaatattc
      841 tggacgccgc cgacgatgac ccccgcgccg atctgcagtg gatcggtccc gccggtcgtc
      901 gccgacggtg ccgacgctcc ctcgagagtc tgaaccagca gatcagagaa ttgtgcccgg
      961 cttgccttat agccggtcgt gttcgcattc gcgatgttgt tgccgattac acccaaatcg
     1021 gtctgatagg cctttaaccc ggtgatagca gtgctgaatg tgctcatcga tcatccctcc
     1081 tgttgtgtag gggcagggct tgccctgccc aaatagggcg cagcaagcag cgcccctacg
     1141 tctcagcgct tatccctgtg atgcgagtga catctgcgag atggcactta agggaaccat
     1201 actcccatcc acgatcagga aaggttgggt ctcttcgaag tgaatgctcg tcaccacgcc
     1261 ggaagcggtg ccggccccag cgacgcccac gccagccgca tccgtagcca ccaccttata
     1321 gagatagcgc ccaggcggca gcggatatcc atccgcgccc agcccgtcga aagtgagctc
     1381 gtaccgtccg gccacctgcg cccccaacgg aatcgtccgg accgtattgc cttgcagatc
     1441 cctgacctgg acggcaaccg tggcagccgc aggcagacta tagcggagcg gagctgggtt
     1501 gttttctcca agctcgatga tggtcccgtt cgctgcaacg acttttccga tatagcttga
     1561 ggccaggacg gcatccaacg acgcgcggtc ggtcccggcg gaggaggtcg agagcttggc
     1621 aagcgatgta ttaatctgct ggagctgctc cagtgagctg aactgggctg tttgtgcgat
     1681 gaactcggtg ccctgcaacg gattcaacgg attttgatta cgtagctggg cgaccagcaa
     1741 cttcaggaag gcatccttcc caaggcctct gtcgagcggg gaaccggtcg cgacgccccc
     1801 accctgcggg ctttgcacgg tggtggcgct cccgcttgtg atctccatcg atcatccctc
     1861 ctttcttaag ccttcagcta tcagcagtca gctatcagct aaaggctacc gctgatcgct
     1921 actgacctca aaaatgcccg taagcggtac cagcgcgctt ccaacgttaa ggaacggctg
     1981 cccgttctcg aactgtactc ccatcactcg cccgaatgca gtactgacgc gagccatcgg
     2041 ttgacctgct gtatcggtgg cgatgaccct gtacacgtaa agaccggact ggagccctcg
     2101 cccatcgaac atcatctgat ggaatcctcc tggctgcggc ccgagttgga cggtcctgac
     2161 tatcgtcccc tggaggtccc gcacctcaat ctggaccgat tcggcagcgg tggggaggcg
     2221 atacagaaga gtcgatggaa ccgacccgcg cagttcgatg gccgagccgt tcgctgctat
     2281 cttccgaccg atcaagtcgg cggctgcgcg ggcacttacc ggcccctgag ggactgtggg
     2341 tagagctgtc gctggggcaa acgtccgtct tgtcgcaagc cgggccgtca gcaggccgac
     2401 agaggcatca gcagcagagc cgctgataac ggttggcgcc aaaccctggg atgagagagg
     2461 accaacaggc ctcgcgtccg atcgagtcga tctcatagac cggctccgct ccaggattgt
     2521 atcgaatgat ctttcggtgc gatcaaccgc cgtactgcta tcgagacctt gttccataag
     2581 ggcggtgata gtcttccctg ccagcgcact cagcaccttg atcccgacgg cggcaagcac
     2641 aggtatcatc ttgggtctcc cctttgtttg aagcagtcag tcgagagctg aagactgctc
     2701 ttagacagtg atatcgatca agctgtccga tcccatcacg gggatcatcg gcggtattga
     2761 tccctggtca tgttggagca tcacctccgg aaggtacccg atggcatgcg agtcttgaga
     2821 tccgttccct gcgtagctcc attggtgaag atccaacccg acctggacgc ccagatcgga
     2881 aatcggaatc ccctgctcca cgagggctat cctgaggcga cccaaatcgt tggtcaacag
     2941 ctcacccgcg acgtgatgtt ggaccttgat ggctgcgaca attccgctgt ccttccacga
     3001 cacctcgatc acaagttcgc caagcgtctg gggatgaaga cggagttgga cgtgtttccc
     3061 ctcattcctc tggcttaatc tcgccgccat aacgataggc tcggctaact gatcggacag
     3121 cgtccgtgga tgccagaacg gcaccacagg tccggatcga agtccctccg tcctggacgc
     3181 agcgctgagc gtacaatcgg catgaagccc tggtctgtcg ctggaaggag atgatgaacg
     3241 gtcaggcgca gagcttccgc tttctgaatc ggaggccgac tcgtcctgct tcagccattg
     3301 cgtagtctcg atcctcggcg actcttgccg tagtgccgct cggtcgtcgt ctgcgttcac
     3361 ctgtgtctga tcccggaatc cctcactatc acgcgcctca gaccccagcg cctccgatac
     3421 accggaggat atcgtgattc gaaccgccgg gcgttggggg aatgacgagc gacctgcggc
     3481 ctcctcggac agcgcaaccg taaccttcgc cgagtctccc aacaaagtcg ctatcggcag
     3541 caagccgccg acctgccccg gtgcctgagc gtcgttcaac cggttaatac cgtcctgaac
     3601 ttcctttgct gaggtcgcgg ggatgttgac ccctccaccc gctgattgct gattgccgat
     3661 cgctgatcgc tcgttgttga caagcggcat gacgagggtc ggtcgcgcgg catccggctt
     3721 agacgtctgg cttgatcctt gcggaacggc cggcggctgc tgaaccgtac cggaaggctc
     3781 agtccatact tccctggctg ataatccggt atccggcgaa ggactgacca tctccggtat
     3841 ggcgctgact atggtgctct cctctgagat atccgtcttc ggataagttg cggcatcacc
     3901 ttcgagaaac acagaccgac gagaggtggt cgtggcggca ggcgctccag gcggccgcgg
     3961 cggcgttaag cctgttccct cagtggaagg cgcgtacgac agcacgacag ccggagcggg
     4021 agccgtgagg tctgcaacgc ggtcgacgtc ggatcgggcc tcgaaccgtt cgatccgaac
     4081 aacctcctgc acacctcggg ccggttctgt cggctgctcc gccaagtgag gatcgtggcc
     4141 tcgaaggtct ccctcactct ggccctctcc gcgccaagag gatacctgga gagagaatgg
     4201 aggtaaggtg gtgcggactt gcatgctcat gagcaggcca tcgccgtatg cggtatgggt
     4261 ctctgcatcg gcgggtaacg tcatgatact cagcccttgc ggacgaagcg aaggtccgga
     4321 agtgacgaga gtcgctccgg tttcttgact cgagaccgct ggcttgaggt gtggatcgat
     4381 ctcgacggag agcaatctcg ttgagtcgac cgatatcgcc acaccaacgg aatccaccgc
     4441 gtggatatca gcggactcga gcatagagcc ggcttcccgg gtaaagagag gttgcgtaga
     4501 cacctccaag aaggttacgg cggcaggcgc agcggccata tccgagggca agagatccgc
     4561 gatgtaatca caacctgccg agggttccag cacggcgctc tgcgtggacc cgctaaagat
     4621 agcgacaaga atggctgcag cgggatcgat attgcgctcc gcctgaccct tctcgctact
     4681 gccgtctgat gaggaccgac acgccgggag acgtttttca cctgtcaaca cgtcgagcgc
     4741 gccgatcgga cctcccggta cagtctcatc agctacctga gccgcaacac cactcccgat
     4801 catttgctcg aataatgaca ggaaatcggc cgccccttgt ggacacatcg tttctgctgc
     4861 aatgccctga ggcgcgctga aaacggccgc gtcggcaacc tgcagcggga tcatggtgtc
     4921 gcctcgccgg cctttttctg gttgatcgtc cggtctactc ccgcatcttc aagccgttca
     4981 ctcagttctt tcgccttctg tccattcatc gcggccagca ctttcgaggc ctgtcgagcc
     5041 ttcatcgcag cgagtacctc cgtcgccatc tcccgcttca gcttgctcag aatcgacgcc
     5101 gcagcctccg gatccattcc ctcgtagagt gtcgccagcc tctttactcc ggcttctcgc
     5161 ctggcatctc gctctttcgt gagcttcgcg ccggtcgcct ttaactcggc gagccatttc
     5221 tcctcgatct ggttctcctc cttccgcttg cgtatctgct cctccagccg gagcagctcc
     5281 gctcgtttct ccgcgagtgc cttctcctga tcgctgatca ctggtcgctg ttcgcctgac
     5341 ggcgacggtg gcgtccctgt cgcggcagca gtctgctgtg gtagatgtga tgcgctcgcg
     5401 ccggacttct gtagcgccgg ctgagccaac ggtttctcct ccggcggctg cagggaagcc
     5461 tgatctgccg gcgccgatcc cgtatcgtga gcctggtctg actcctgcac ggtcgagggc
     5521 tgatcgctga ttgccggctg ctcatcctgt tgaggggcag cggctggacg gccaaagcgc
     5581 gggagaattc cctgctgcag gacaccggtc aggtaagagg caagcagcaa caccgcgacg
     5641 aaatatccgg catacagcgc gattcgactc ttcacgacac agaccccctg aacgcgttcg
     5701 ccgcccttcg tccgcccgct acctcgtcga tctcagcctg ttgctcacga gctatctcac
     5761 tgcgaaatac cctgatcttc cgcttcttca ggtgctcaac gatctgttcg tctctcgatc
     5821 gttccagcaa catgcgccga agtctgaccg tctccgcctg ttcggcggcg agcatcgctt
     5881 cgagcgccct gatctgtttc ccaacgccca acaggtaggc gcaacgaacc gcgagcgagg
     5941 atgattcagg ctgagcgatg ttctgtccca ccgccagttc agccagtgct tccgcttcgg
     6001 catccctgag tttcgccagc cgttcgcgta atgcgccgca ggctcgctgt cgctcagcca
     6061 atgcagcctc cgcctgatct gccttcgcct ctctgacggc gagaaccgac tgaagggaaa
     6121 accgaaatcg cttcatcgtt ttcgtcctct actatgcgac cgcttgagtc gccgccacac
     6181 cggttctggc cattgaagca gcggccagat acgacgcctc gaccctgggg tccggtttct
     6241 tgcgccgcgg cggcgcctcg gcgccggtct ccggcgcgaa cagggtcacc aatcgctcca
     6301 ccattcccgg aaagtctgat cgttcgtcgg acgattgccg aagaaacgcc tcgatcggcg
     6361 tcatgtaccg taaggcttcg tccaccttcg gattgcttcc ggacacatac gccccgatgt
     6421 tgataagatc ctgggcctgt cgatgagccg ccagcaactc ccgaagcttg gacgccgcaa
     6481 ctctcacctc ccggggcgcc accaccgaca tcaaccggct gacgctcccg agaaaatcga
     6541 ccggggggta gtgattccgc tcggcgagct ccctggtgag aacaatgtgg ccatccagaa
     6601 tggcccgagc gtgatcggcc accggttcgt tcatgtcgtc tccctcaaca agaatggtgt
     6661 acagccccgt aatactcccg ccatgatccg ccgtcccggc ccgctctaag agtccaggca
     6721 acatggcaaa tgccgaaggc ggatatccgc gcgtcgtcgg aggctctccc accgacaggc
     6781 cgatttcccg ctgagccagc gcgaaccgcg tgaggctatc catcatcagc agaacatgct
     6841 tgtcctgatc ccgaaaatat tcggcaatcg ccgtcgcaac gtacgccgcc tgtcgtcgca
     6901 gcagggaggg ctcattggag gtggccacta ccaccaccga gcgcctcagc ccatcctctc
     6961 ccaactcctt agtaataaac tcccggacct cccgtccccg ctctcctacc agggcaatga
     7021 cgttcacatc ggcctcggta tagcgggcga tcatgccgag gagggtgctc ttcccgacgc
     7081 cgctccctgc aaagatgccg acgcgctgtc ccttaccaca ggtgagcagt ccatcgatca
     7141 cccgaattcc ggtcgccagc ggctcgatga taggggcgcg cgtcataggc gaagggggat
     7201 gacggtgaac agaacagcgg gcctcgacct ccaccggagc cttcccgtcg ataggacgac
     7261 caagaccgtt gatgacgcgg cccagcatcg atcgtcccac cggcacttca aacggtcggc
     7321 cggccgcgac cacctcgctt cccggacgga tcccgtggat ctcgcccagc ggcattaaga
     7381 gcaggatctc cccgtgaaac cccaccactt cagcccatac atcgtgcctc cccgccgaac
     7441 ggattcggca cagctcaccg ataaacgcct cctgccctac cgcctctatc accagaccga
     7501 ccaccttggt cacctttcca agcgatcgaa tgaggttcgc cttatccacg cgagcgcgcg
     7561 cacgacccca gtcgaatctg gcgtcgctca tgagatctca tctcttccga gcaggcgagc
     7621 agcctcatga agttggctct ccacgcgcgc atccacaaga ccggactcaa cctccaccac
     7681 gcagccccag gaggagatcg cgggatcagg gatgattcgg gccgactccg gtgagccgag
     7741 cagttctccc cgatgctcca tcagtagttc gtagtcaccg ggattgaccc gaatagccat
     7801 aatctttcgg ctcccagcga cttccagggc gactctggca aactgtacgg ccgcatctcg
     7861 gcgcgccccg agttcggatc ggagcactcg ctccgcgacg gcaacagcaa ggcggaccaa
     7921 ctcctcctcc gcattcgtga tgaccgcttg gcgagcggac gcaacctgtc gacaggtctc
     7981 ctgcagcaac gtctccaacg gctcaaaccg acgccgcatc tcgtctatcg catcctgtcg
     8041 gcccttggcg cgaccctccc gttcggcctc gcgctcgatc tgttgcgccg cttcacgagc
     8101 cgagagtatg atcgcctcag cctcgcgctc ggctgctcgg atcgactcat atcccgactg
     8161 tgcaggctcg tgctctgtcg cccacgcccc gccggccgat cggcccgcgc tatacaccaa
     8221 ctcctcaaag gaggctcgct cccatccccc aactccggcc gtcacaatct tagacaatga
     8281 tctcctccgc gccaccgcca cgcccctgta caataatctc acccgcctct tcgaggcggc
     8341 gcacgatcgc aacaatcttc tgctgagacg cctccacatc tcggagtttc accggcccca
     8401 aatactcgat ctcttccttg agcagaccgc ccgcccgctc cgacatattt ttgaagagtt
     8461 tctccgccac ctcaggaccg gctgccttca tcgcaagagc aagttccttc tgttccacct
     8521 ccttaagaac gcgctgaatg ccacgatcgt ctaccaggac gacatcatca aaggtgaaca
     8581 tcagtttgcg gatctcgtcc gccatataag ggtcgttctc ctgaatgacc tgaaacacac
     8641 cgcgttcgct atttctatcc atggaattca ggatctccgc tacctgcttg accccgccgc
     8701 tgatagagac ctcctggttg actaccgagg aaagacggtg atccaggatg tgttcgatct
     8761 caccgatcac ctcagggctc gtcttgtcca tgttggcgat gcgcataacg acatccgccc
     8821 tgaccgcttg cggtagaccg gccaggacgc tcgcggaggt cgtcggctca aggtgggaca
     8881 ggacgacggc aatggtctgt gggtgctcct gtcggactga gtcaagcaaa aaccgggggt
     8941 ccaccttctt gagggcactg aaaaagctgg cgcccttcag gccctggagg cgttcaatga
     9001 tcgccgacgc cttattcctg ccgacggcct tttcaaggac atcctgcgcg tagcggatac
     9061 caccaatcga cataaactct tgcgcgcggg ccatctgctg gaactcctcg atgaccgcaa
     9121 tcgacgcctt cgcggagaca cccttcatcc tggtaatctc accggtgatc gcctcgattt
     9181 cctcgtcctc aagctgcttg aagatttgcg cagccgactc gactccgagc gtcagcagca
     9241 ggacagcggc cttttgggga ccggtcaatg tctcatggga ggtcatgacg gcctcttcac
     9301 catccaggcc ctgacgagct gggcgacgac attcgggtta ttgcgggcca gttccatcag
     9361 ccccgccctc agcggatcgt ccgaggcgac gtccagggcc ggggcctgat ccggtccggt
     9421 ttcaatcgcg gtgggacttg gttgcgacat tgaaagcatt cccggcgcgt caaaggtcgg
     9481 aagccggcgc tccttcaacg aacgaatcag cggccgcagc ccaaacacca gtacgaggag
     9541 gacaccgaca accatcaaga tcggtttcac cagcgagtac cagaagtcct tgcgctccgc
     9601 ctcctccatg agggcccgct ccttctccat agccgaggta tcgaagggaa actccaccac
     9661 ctcaatctca tccccgcgag ccgcactaaa tccgactgca ttttttacgg ctaccttgat
     9721 cttctcgagt tcatcggact tccgagggac atactctttc cgctcgccgc ccttaccgtc
     9781 cggaactgcc ttgtaggtcc cgtccaccat gaccgctacc gacacccgct tcaactcccc
     9841 aggcgaaaag accttccgtt caaggacctt gctcacatcg tattgaaccg tttcggcctc
     9901 ttttgtcgat ttcgcggtgc tcttacccga ctgcgactgc gtagcggctt gatccggaac
     9961 atttgaggca acccctgcta ctgccgtagg agtcgcggta gagctctgct gggtttctgt
    10021 cgtccgctgt tcgctcttaa tgacgccgcg cgggtcaaac cgctcctctg tccgctctac
    10081 actggtaaag tccatctgag cggcgaccct caccgtcgct ttattcgggc caaggatctc
    10141 ttcgagcatg gtccggacgc ggcgctcaag ctcgacctcg acggcctcct gcacctcgaa
    10201 ctgtccggta cttcctgagc cgagcctgcc ttcggccggc cgcgcgatca acttcccgga
    10261 tgtatccacc accgtcaccc gatcggcatt gagcccctct accgagctcg agaccaggtg
    10321 tacgatcccc cgtacctgct caggcgtcag gcgagcaccg ggacggaggt tcaggacaac
    10381 tgaagcagtc gcgggcttct cctgctcggt aaacacagaa ggctggggga ggacaaggtg
    10441 aacacgagcc gccgtaacct ctttgagttg cccgattgtc ctggcaagct caccttggag
    10501 ggcgcgctgg taattgagtc gctgaacaaa gtcggtcgtt cccagtgtcg tgcggtcaaa
    10561 gatctcgaag cccacccctc cgccttgcgg cagaccacgg gtcgccagat gcagtcggat
    10621 ctcgtggacg acggccgatg ggacaaggat cctggtcccg ccgtcaacga cccgatagga
    10681 caccttgctg cttttcagct cgtcgacgat agcaccggca tcggaggagt tcaactgcga
    10741 gaagagggtg gtcatatcag gacggtgcgt cgagaaggca atccagagg
//
LOCUS       DATMXI010000310         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_179902, whole genome shotgun sequence.
ACCESSION   DATMXI010000310 DATMXI010000000
VERSION     DATMXI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179902"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VN444_06020"
     CDS             <1..188
                     /locus_tag="VN444_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21401.1"
                     /translation="SNDLTAPSKLARSLSIGMTPVMVPLRPSSEHILIVRAPGARDQH
                     WCHSILLFLDKKIERRL"
     gene            244..774
                     /locus_tag="VN444_06025"
     CDS             244..774
                     /locus_tag="VN444_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HWR21402.1"
                     /translation="MTIAQMDPAVTLADLQASKPEKVTIVLLSGDMDRAMAAFIIATG
                     AAAMGMQVTVFFTFWGLNAIRRKGATSSAKDWLRQLFGLLNKGGADSLPLSRFHFWGL
                     GTKMMQQVMRQNRMPGVPELMETALGLGVHFIACTTTMGLMGITKDTLIEGIDQFAGV
                     TTYLAEAKQGSVNLFI"
     gene            817..1062
                     /locus_tag="VN444_06030"
     CDS             817..1062
                     /locus_tag="VN444_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWR21403.1"
                     /translation="MIQADVKLDTLGYFCPMPIIMTLKKIKELKLGQVLEVVSDDEGI
                     KKDMPAWCDTTGHQMVGLEEEQTKAGTVYKAFVKKTK"
     gene            1355..>2235
                     /gene="ftcD"
                     /locus_tag="VN444_06035"
     CDS             1355..>2235
                     /gene="ftcD"
                     /locus_tag="VN444_06035"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWR21404.1"
                     /translation="MDRIVECVPNFSEGRNQTTVHALARAVESVPGVWLLDQTMDRDH
                     HRSVLSFAGEPDAVAEAAFRAIRVATDLIDLREHRGVHPRVGATDVVPFVPLRGATMQ
                     DCIHLARRLGKQVGAELEIPVFLYEQAALHRDHAPLESVRRGGLQGLAFRMASDPDWT
                     PDFGPPHLHKTAGAIVIGARAPLIAFNVNLRSTDLRLARSIAKDIRQSNGGLPHLKAI
                     GVELASRRLVQVAINLTDYVITPIHVAFEAVRTSAAAQGVAVAGSEVIGLVPQAALLQ
                     AAAHGLALEQFDELQVL"
BASE COUNT          512 a          612 c          641 g          470 t
ORIGIN      
        1 catcgaacga tttaactgct ccttccaagc tcgctcgttc tctctccatt ggaatgacac
       61 ccgtgatggt ccccctgcgc ccgtcgagcg agcacattct gatcgtgcgc gctccgggag
      121 caagggatca gcactggtgt cattccatcc tcctcttcct tgacaagaaa attgagcgga
      181 gactatagtc aaattcctat tgaaatcgcc aaattcatgc ggacatgaga ggatagacga
      241 cgcatgacaa ttgctcagat ggatccagca gtcacgttgg cagacctcca ggcttctaag
      301 cccgagaagg tgacgatcgt gctgctaagc ggcgacatgg accgggcgat ggcggcgttc
      361 atcatcgcca ccggggctgc tgccatggga atgcaggtca cagtattttt caccttctgg
      421 ggtctcaacg ccatccgccg gaaaggagca accagctcag ccaaggactg gcttcgtcaa
      481 ctgttcggcc tgctcaataa aggaggcgcg gacagccttc ctctctcgcg gttccatttc
      541 tgggggttgg gcacaaagat gatgcaacag gtcatgaggc aaaaccggat gcctggtgtg
      601 ccggaactga tggaaacagc cctgggcctc ggtgttcact tcattgcctg tacgaccacc
      661 atggggttga tggggatcac gaaggatacg ctcatcgagg gtatcgatca gttcgcagga
      721 gtgaccacct atcttgcaga agccaaacaa ggcagtgtca atttgttcat ttgaacaagc
      781 gggacaagcg cgaaaggcga gacgtggagt gaagagatga ttcaagccga cgtgaaactc
      841 gatacgcttg gctacttctg cccgatgccg attattatga ccttgaaaaa gatcaaggaa
      901 ctgaagctgg gccaggttct tgaagtcgtc tcagacgacg agggcatcaa gaaggacatg
      961 cctgcctggt gcgacacgac cggccatcag atggtcgggc tcgaagaaga acagaccaaa
     1021 gcgggaacgg tctataaagc atttgtgaaa aagacaaagt aaccgtacgg acagagcttt
     1081 cacaggctgg aaccttccat aaacagtctc aatgtcaaca tagcctcagg atgctcaaaa
     1141 aggccgttca gcaaggccgc agcgaatgaa gaaggctgag gtcgaggtta aggttaagtg
     1201 tcgaaccggt ttttgtttgc tcaaccttag cctcagcctt aaccttccca taacgctggc
     1261 ggtctttttc agcatcctat tagtggagag agaagccgga gggctgacgg tcagacctcc
     1321 ggcttcgttg tgttgatatg tgatgaggcg gggcgtggat cgcatcgtcg aatgtgtgcc
     1381 gaatttcagc gagggacgaa accagacaac ggtgcatgcg ctggcacgtg ctgtggaatc
     1441 tgttcctggg gtctggctgt tggaccagac aatggatcga gaccaccatc gctcagtctt
     1501 gagctttgcc ggtgaaccgg acgccgtcgc tgaagccgcg tttcgcgcga ttcgcgtggc
     1561 gaccgatctc atcgatcttc gagagcatag aggtgtccat ccgcgggtgg gggcgacaga
     1621 tgtcgtgccc tttgtcccac tgcgcggggc aacgatgcag gactgtatcc atctggcaag
     1681 gcgattgggg aagcaggtcg gggcagagct ggagatcccc gtcttcctct acgaacaagc
     1741 ggccttgcac cgcgatcatg cccccctcga atcggtccgc cgaggagggc ttcaaggcct
     1801 cgccttccgc atggcctccg atcctgactg gacaccggat tttggcccgc cccacctcca
     1861 caagacagcc ggcgccatcg tcatcggcgc gcgcgctccc ttgatcgcat tcaacgtgaa
     1921 tctccgctcg accgacctga ggttggctcg atccattgcg aaagacattc gccaatcgaa
     1981 cggaggactg ccccatctca aagcaatcgg cgtggagtta gccagccgac ggttggttca
     2041 ggtggccatc aatctcaccg attatgtcat cacccctatc catgtcgcct ttgaagcggt
     2101 ccgtaccagc gcggccgcac agggcgtggc agtagctggg agcgaagtaa tcggccttgt
     2161 cccacaagcg gcactgcttc aagctgctgc acatggcctt gcgctcgaac agttcgatga
     2221 attacaggta ttgga
//
LOCUS       DATMXI010000311         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_290182, whole genome shotgun sequence.
ACCESSION   DATMXI010000311 DATMXI010000000
VERSION     DATMXI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290182"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1739)
                     /locus_tag="VN444_06040"
     CDS             complement(99..1739)
                     /locus_tag="VN444_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWR21405.1"
                     /translation="MPQIDSRVEPPSAMPDERISAGEQLFETWRKRLGAILTLPAFFL
                     TYVLCSGLRPAGQKLAAILATVTVLWISEVIPLPVSALLGAVPEEPGKSPAAVVLAYF
                     ADPIVFVFIGGFMIARAMTIHGLDRRIALGFLSIPWVGSSPARTMVGLGLVTAVISMW
                     VSNSATTAMMLPIALGILRALHDVRVARGLAQDSSDVLNWPFATGMMLMVAFSASIGG
                     IGTPVGSPPNLIGIGLIRSATGIDISFFRWMAVAVPLLLVMGLTLFTLLYLLHPAGKA
                     ADRAACNQGDGTAQVTPNALTADIGSHLLHYIRGERSKLGPWTWGQINTVVAFGVAVT
                     LWVMPGIMQLPWLAGSWWGLWMSTHLPESIVAISAAILLFLLPVNLAQWEFTLTWPEA
                     VKIDWGTILLFGGGLALGSLMFKTGVAEAIGNALTRHLGVSSLWGLTGLSTAMAIVMS
                     EAASNTASANMIIPVVIAIAQAAGVNPLPPALGACLGASFGFMLPVDPPQCHRIRLRP
                     RPAPENDTRRHPVGYRRLLHYLGRLVRAASDTGASVTT"
     gene            complement(1942..2265)
                     /locus_tag="VN444_06045"
     CDS             complement(1942..2265)
                     /locus_tag="VN444_06045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWR21406.1"
                     /translation="MAISRFEVYLVNLDPTVGHEIKRVRPCLVVSPDEMNHHIRTVIV
                     APMTTKGRAYPTRVPCRFKGKTGQIVLDQLRTVDSVRLVKRLGHISQKTVSDVLAVLQ
                     EMFAP"
     gene            complement(2256..2513)
                     /locus_tag="VN444_06050"
     CDS             complement(2256..2513)
                     /locus_tag="VN444_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR21407.1"
                     /translation="MKARIVQIGNSQGIRIPKPLIKQTGLGEEVEIQVEGNRLIIAPA
                     GRPRCGWDSAFEKMACSGSDVVLDTKAHVPTEWEEDEWEWQ"
     gene            complement(2697..2882)
                     /locus_tag="VN444_06055"
     CDS             complement(2697..2882)
                     /locus_tag="VN444_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR21408.1"
                     /translation="MAADNLGQFPEIGHTFRSEAEGDIRIVLYGHYRIAYLLRPSSSI
                     DILGVFHGAIDIDRYLP"
BASE COUNT          678 a          919 c          872 g          641 t
ORIGIN      
        1 ggagagtccc ggtgacgccc aggaacgtgg cggtcttgcc cagggtggct gccgtgcgaa
       61 gacgttcggc cagagcgacg ttacctggtt gcgaatcctc acgtggttac agaagcccca
      121 gtatctgatg cagcacgtac aagccgcccc agataatgaa gaagcctgcg atatccaaca
      181 ggatgccggc gcgtatcatt ttcgggagcg ggacgaggcc tgagccgtat acgatggcat
      241 tggggggggt caacggggag catgaacccg aagctggcgc ctaggcacgc ccctagggcc
      301 ggcggcaacg ggttgacgcc ggcggcctgc gcaattgcaa tcacaaccgg aatgatcatg
      361 tttgccgacg cagtattgct cgccgcctca gacatcacaa tcgccatcgc ggtcgataag
      421 cctgtcagtc cccacaggga gctgacgccg aggtgcctgg tgagggcatt gcctatcgcc
      481 tcggccacgc cggttttgaa catcaatgaa ccgagtgcca acccgccgcc gaacagtagg
      541 atcgtacccc agtcgatctt gacggcctcg ggccacgtta acgtgaactc ccattgcgcc
      601 aggttgaccg ggagcaggaa caggaggatc gcagcgctga tcgcgacgat cgactccggt
      661 aggtgtgtgc tcatccatag gccccaccag gatcctgcca gccacggcag ttgcatgatg
      721 cccggcatca cccacagtgt gaccgcaacg ccgaatgcga cgaccgtgtt aatttgtccc
      781 cacgtccacg ggccgagctt actccgttcg ccacggatgt agtgcagcaa atggctccct
      841 atgtcggcgg tgagagcatt aggtgtgacc tgagctgtac cgtcaccctg attgcacgcc
      901 gccctgtccg ccgccttgcc cgcaggatgc agcagataga gtagcgtgaa cagcgtgagt
      961 cccatcacca ggagcagcgg gacggccact gccatccagc ggaaaaaact gatgtcgata
     1021 cctgttgctg aacggatgag cccaataccg atcaggttgg ggggcgatcc gaccggcgtc
     1081 ccaatgccgc cgatcgacgc gctgaatgcc accatcagca tcatgcccgt ggcgaaaggc
     1141 cagttcagga catcgctcga atcctgcgcg agccccctgg cgacgcgcac atcgtgcagc
     1201 gcacgcagaa tacctaatgc aatcgggagc atcatggcgg tagtggcact gttgctgacc
     1261 cacatggata tcacagcggt caccaaccca aggcccacca tggttctcgc cggagaggaa
     1321 ccgacccacg gaatcgacag gaaccctaac gcgatacgac ggtcgagtcc gtggatcgtc
     1381 atcgcccgag cgatcatgaa ccctccgatg aacacgaaga cgattgggtc ggcaaagtag
     1441 gccaacacca cggccgccgg cgatttgcct ggctcttccg gcaccgcgcc cagcagcgcc
     1501 gacacgggca gcgggatgac ctcgctgatc cacaacaccg tcacagtcgc aaggatcgca
     1561 gccagcttct gacctgccgg tctgagtcca ctacacagga cgtaggtcag gaaaaacgcg
     1621 gggagcgtga gaatcgcgcc tagccgtttt cgccaggttt cgaacagttg ctcaccggcg
     1681 gagatccgtt cgtccggcat tgctgagggg ggttcgactc gcgagtctat ctgcggcatt
     1741 gcatattctc aaatcaagga gttacgccgg ttcgacccgg ttctcggttc tcgttctccg
     1801 ttctcgtcaa gacgtaaccc ccttcttttg tgacatgcgg cccgcggcgc gtaagaccgc
     1861 actgggcggc atgataccag acgataccct tgtcgcgcgc ggtttgtgcg ttgcagtgta
     1921 gccgctggtt agggtacagc ttcaaggtgc gaacatttcc tgtagaaccg caagtacgtc
     1981 cgagactgtt ttctggctta tgtgtcccaa tctcttcact aggcgcactg agtcaacagt
     2041 ccgaagttgg tccaagacga tttggcccgt cttgcccttg aacctgcaag gaaccctcgt
     2101 gggataggct cgcccctttg tcgtcatagg ggctacgatc accgtgcgga tgtgatgatt
     2161 catctcgtct ggtgagacga ccaggcacgg acgtaccctc ttgatctcgt ggccaaccgt
     2221 cgggtccaaa ttcacaaggt agacctcaaa acggcttatt gccattccca ctcatcttct
     2281 tcccactctg tagggacgtg tgccttcgta tctagtacga catcgctacc cgaacaggcc
     2341 atcttttcga aggctgaatc ccatccacaa cggggacgac ctgcgggagc gattatgagc
     2401 cgattgccct cgacctgaat ctccacctct tcgcccagac ccgtttgttt gataagaggt
     2461 ttcggaatgc gaataccctg ggaattgccg atttgtacta tacgtgcttt catgattaat
     2521 gctcctcctt gtaatcacat tgtaatcact acatggagtc gtgtcaagcc gtcagtttcc
     2581 ccggaggtgg atatggaagc cctattcctg tgatcctacg cagagctaac ctgcgaacta
     2641 taaggtgcga agcgccgacg tggcagtcag gttgagcagc gggttatgct cacgaattac
     2701 ggcagatacc tgtcaatgtc gatcgcgcca tgaaacacac ctaagatgtc gatgctggat
     2761 gatggcctta gaagatatgc gatccggtaa tggccataga gtacgattct aatgtcgccc
     2821 tctgcctcgg agcgaaatgt gtgcccgatc tctggaaatt ggccaaggtt atccgcagca
     2881 atgtagtcgt atacttcgtg gacgaactat acgatattgg cgcgtcacat gcgacaagat
     2941 actcgtgaca cacccctagg agcatccttg aacaaaacgc ttgcacagga acccactgtt
     3001 gtatagcctg tttagtcaga tatggatgat gcccaatatc tcaggcgcgg acctgtagcc
     3061 tccttcaacc cagatcttcg ctggccagca gggtgcttgc gggtagtcat
//
LOCUS       DATMXI010000312         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_136271, whole genome shotgun sequence.
ACCESSION   DATMXI010000312 DATMXI010000000
VERSION     DATMXI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136271"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="VN444_06060"
     CDS             <1..1737
                     /locus_tag="VN444_06060"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWR21409.1"
                     /translation="KIAFLSPSPELSAQVANAVAENFIEQSIEQKLDATKYAGDFLAK
                     QIRDVRIKLESSDELLQQFAKQKQYLVLDEKQEQTTKQLSLLTDALMKARSERLAKEA
                     LHRQTQGQDFQSLPSVLENSLIAGLKTEYFRLQAEYRKLSETFLPDYPRMVALKSNIE
                     EVKVRLDGEVQKIVDGLRSSYEAAVKAEHLLQSSVDTQKQVTLKTNEDSIQYNILKRE
                     VDINRELYAGLLQRKKETAVSAGLDSTNIQITDRAKVPISPDRPRKLLNLTLGVVLGL
                     SLGIGLAFFADYFDNTVNNIEEVESTFALPILGAVPALASVGQKKRLKGTTNGQEEKS
                     FELIMHQDRASLLSEAIRSIRTSILFSLPENTPRLLLVTSAEPGDGKTGVAVNLAIAL
                     SQLGGDTLLIDADMRCPDCHRILGLDRTPGLSNFLVGDAELSAVIKPTAIPTLLYLLP
                     AGQSPLNPAELLGSERMSATLDLLTQQFRYVIIDSPPVLGFTDSVLLSTLVEGTLFVI
                     RAGKTSRDAAQRAVGTLDAVNAKILGVVLNHLNLQGNGYSYYQDYHDYYRRKSEHQGV
                     MAKEQRDRNNTP"
     gene            1866..>2714
                     /locus_tag="VN444_06065"
     CDS             1866..>2714
                     /locus_tag="VN444_06065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWR21410.1"
                     /translation="MPLVTVSNMQDGQQPRIPFHLPSIGEEEIAEVVEVLRSGWLTTG
                     PKARRFEEDFAAYVGAKHAIAVNSCTAALHLALEAVGVGPGDEVILPTYTFAATGEVV
                     AYLGARPVLADCRPDTLNIDVVTVAPLITPKTRAIIPVHIAGQACDMDPIMELARKHG
                     LHVIEDAAHALPTTYTGRMVGTIGDLTAFSFYATKPITTGEGGMVTTMRDDHAARMKR
                     MSLHGLSGDAWNRYSDKGHWYYEILDFGFKYNLTDLAAALGIQQLRRSDDFYKRRREI
                     ARMYSEG"
BASE COUNT          626 a          745 c          782 g          561 t
ORIGIN      
        1 aagatcgcct tccttagccc ctcaccggag ttgtccgccc aggttgccaa cgcggtggca
       61 gagaacttta tcgagcagag catcgagcag aagctggacg cgacgaaata tgccggcgac
      121 ttcctggcga aacagattcg agacgtgagg atcaagctcg agagctccga tgagctgctc
      181 cagcagtttg ccaagcagaa gcaatatctc gtgctcgatg aaaaacaaga acagaccacc
      241 aagcagctct cactgctcac tgatgccctg atgaaagcgc gcagcgagcg gctggcgaag
      301 gaggcgctgc atcggcagac tcagggacag gattttcagt cgctcccatc ggtgttggaa
      361 aattcgctga tcgctggctt aaagactgag tatttccgcc ttcaggccga atacaggaag
      421 ctctctgaga cgttcttgcc ggactatccc aggatggtgg cgctgaagag caatatcgag
      481 gaagtgaagg taaggcttga tggtgaggtc caaaagattg ttgatggtct ccggtcgagt
      541 tatgaggcgg ccgtaaaagc agaacatctg ctccaatcgt cggtggatac gcaaaaacag
      601 gtaaccctca agaccaatga ggattccatt caatataaca ttctgaagcg agaggttgac
      661 atcaaccgcg aactgtacgc cggcctgttg cagcgcaaga aggagacggc cgtctctgct
      721 gggctcgatt ccacaaatat ccagattact gaccgggcaa aggtccccat ctcacccgat
      781 cgtcccagga aattgctcaa cctgacgctt ggggtcgttc ttggtttgag cctggggatc
      841 ggccttgcgt ttttcgccga ctacttcgac aacactgtca ataatatcga agaggtcgaa
      901 tcgacgttcg cattgccgat tctgggggcc gtgccggcat tagcttccgt cggacaaaag
      961 aagcggctga aaggaacgac caacggccag gaggagaagt cgtttgaatt gatcatgcat
     1021 caggatcggg cctcgctgct cagcgaggcc attcgaagca tccggacctc aatcctcttc
     1081 tcccttccgg agaacactcc caggctgctc cttgtgacca gtgcggaacc gggcgacggc
     1141 aaaactggtg tcgcagtcaa tctcgctatc gcgctgtcgc aacttggtgg cgacactctg
     1201 ctgatcgatg cggatatgcg ctgtccggat tgccaccgga tcctggggct ggatagaacg
     1261 ccgggactgt caaactttct tgtaggggac gcggagctta gcgctgtcat caaaccgacg
     1321 gcgataccca cccttctcta tctgctaccg gccgggcaat ccccgctgaa cccagcagag
     1381 ctgctgggtt ccgagcggat gagcgccacg ctggatttgc ttactcagca gttcagatat
     1441 gtgattatcg actcgccgcc cgtactgggg tttaccgaca gcgtcctgct gtccacattg
     1501 gtcgagggta ccctgtttgt catccgggca ggcaaaacat ctcgagatgc cgctcaaagg
     1561 gccgttggaa cactcgacgc agtcaatgcg aaaatcttgg gcgtcgtcct gaaccacctg
     1621 aatctgcagg gtaacggata ttcgtactac caagactatc acgactacta tcgtaggaaa
     1681 tcagagcatc aaggtgtcat ggcgaaggag caacgcgacc gaaacaatac cccatgagcc
     1741 tcaccctccc ccttccgagg gggatgtagg ggcggggttt atccccgccc atcccacggg
     1801 agggcgtcaa gccctccttt acaacaaaga agaggttgag cgtgagggat ggcaagagat
     1861 agcgaatgcc gcttgttact gtgagcaaca tgcaggacgg acaacaacca aggattccgt
     1921 tccacctccc ttcgatcggt gaggaagaga ttgccgaagt cgtcgaagtg cttcgctccg
     1981 gctggctgac gactggtcca aaggccagac gattcgagga ggattttgcc gcctacgttg
     2041 gggccaagca tgctattgcc gtaaactctt gtactgccgc gcttcatctg gccctggagg
     2101 cggttggggt cggtcctggt gacgaggtga tcctgcccac ctatacgttt gccgccaccg
     2161 gagaagtggt ggcttacctc ggggcccgtc cggtcctggc cgactgccgg cctgacacct
     2221 tgaatatcga tgtggtcacc gtggcgccgc tgatcacacc gaagaccagg gccatcatcc
     2281 ccgttcacat cgccgggcag gcatgtgata tggatccgat catggaactt gcgcggaagc
     2341 atggtcttca cgtcattgag gatgctgccc atgcccttcc gaccacatat acgggaagga
     2401 tggttggaac cattggtgac cttacggcct tcagctttta tgccaccaag ccgataacca
     2461 ccggagaggg cgggatggtc acgacgatgc gcgacgacca cgccgccaga atgaagcgga
     2521 tgagcctcca tggtctgagt ggcgatgcct ggaaccggta ctccgataag ggacactggt
     2581 actacgagat cctcgatttc ggattcaaat acaacttgac cgatctggct gcggcgttgg
     2641 gaatccagca actcaggagg tcggatgact tttacaagcg acggcgggag attgctcgga
     2701 tgtatagtga gggc
//
LOCUS       DATMXI010000313         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_59505, whole genome shotgun sequence.
ACCESSION   DATMXI010000313 DATMXI010000000
VERSION     DATMXI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59505"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN444_06070"
     CDS             <1..272
                     /locus_tag="VN444_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWR21411.1"
                     /translation="EILSGIRDEAQFRELEVHLAAFPDLPLTTADHVTAAQFFNLCRT
                     KGIQGSNTDFLICAVAIHHTLAIFTTDNDFTLYAARLPIVLHGRT"
     gene            333..878
                     /locus_tag="VN444_06075"
     CDS             333..878
                     /locus_tag="VN444_06075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWR21412.1"
                     /translation="MDENSVAAVLGKLDFEIFVLTAAHQDRCSGQIVCWVVPATIVTK
                     VPRILVGVGRTTFTRELIEVSRRFALNLLGKDQWPLVAHFGFRSGRDMDKFATVPCER
                     GVTGSPILPGTVGYLECEVRTVLDTGAHLFYLADVIEGKVVADRSPLCLHHLPELLPP
                     QDFATMRRLLEHDAQCNLGLL"
     gene            complement(1200..1925)
                     /locus_tag="VN444_06080"
     CDS             complement(1200..1925)
                     /locus_tag="VN444_06080"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWR21413.1"
                     /translation="MIIEVAFSRLDPACRSASGRAVAVIDVIRATTTITMALHHGCAG
                     IIPVRTLSEARAVAQRLGGGALLAGEREADRAVGFELGNSPAEYGRERVKGRVVVLTT
                     TNGTRAFQAAAGAQAIIACSFLNVAAAARWLAGTGLDILIVCAGTHGRFCLEDAVGSG
                     MLIDRLLSISTRALELSDAARASHQLFTTYRDDLLGMLRGCEWGKEIIRKGFGVDLEI
                     CAQVDLTDVVPVMQKDRLVAKRA"
BASE COUNT          462 a          686 c          585 g          479 t
ORIGIN      
        1 aggaaatcct ctcaggcatt cgtgatgagg cgcagttcag ggaactcgag gtccatctgg
       61 ctgctttccc ggacctacca ctcacaaccg cagatcacgt gacggcagcg cagttcttta
      121 acctctgccg cacgaaaggg atccaaggct caaacaccga ttttctcatc tgcgccgtcg
      181 ctattcacca cacgctcgcc atttttacga cggacaacga ctttacgtta tatgctgcac
      241 gccttccgat cgtattgcat ggcaggacct gaggcatcac acaaccgcct cttgcgctag
      301 aaaagggcaa tcgtaatcgg taaggagatg ccgtggacga aaacagtgtt gcggcggtac
      361 tcggtaagct ggattttgag atctttgtcc ttacggctgc ccatcaggac cgatgtagtg
      421 gccagattgt gtgctgggtg gtgccggcga cgatcgtcac gaaggtcccc cgcattctgg
      481 tcggggttgg caggacgacc tttacccgcg agcttatcga ggtcagccga aggtttgcgc
      541 tgaacctact cgggaaggat caatggccgc tggtggccca cttcggtttc cggtccggcc
      601 gggatatgga caagtttgcg accgtccctt gtgagcgcgg ggtgactggc agcccaatcc
      661 tgccagggac tgtcggctac ctggagtgcg aggtcaggac agtcctcgat accggagctc
      721 acctctttta cctggctgac gtcattgagg ggaaggtcgt ggccgaccga agccctctct
      781 gcctccacca cttgcccgaa ctgttgcccc cgcaggattt cgccaccatg cggcggctcc
      841 tggagcatga cgcccagtgt aatcttgggc tcctctgaaa aagcagtaca gccccttagg
      901 caggcggcag gacctaaagg gctgtacact cacctcgaca tcttgcgaat ttgaacgctc
      961 aagcatttct acttccgtac cgcccccacg gattacctcc tctccatggg gcacgcttat
     1021 acacatcgcc gccttcacaa taaatatagt aaccaacctc attcctgtca aggcagcaag
     1081 ctgtctggcc tgcggggaga tctatctgat ccgtctcgtc tgttccgtct atttcgtcat
     1141 agaccaaatg accaaataga cgaaatggcc agatagaccg gatggccgac cattgcccct
     1201 catgcgcgct tcgccacaag acggtctttt tgcatgacgg gcacgacgtc ggtcagatcg
     1261 acttgcgcgc agatctccag atccacccca aagccttttc gaatgatctc ttttccccat
     1321 tcgcagcctc ggagcatccc aagcaggtcg tcccggtagg ttgtaaagag ttgatgggag
     1381 gccctggcgg cgtcgctaag ctcaagcgca cgagtagaga tgctgagcaa gcgatcgatc
     1441 aacatcccgc tgcccacagc atcttccaaa cagaaacggc catgcgtacc cgcgcataca
     1501 ataaggatgt cgagcccggt tcctgcgagc cagcgcgcgg cggcggccac gttcaggaat
     1561 gagcaggcaa ttattgcctg agctcctgcg gcagcctgga atgcgcgggt tccgttggtt
     1621 gtcgtcagga caactaccct acctttgaca cgctctcgcc catattcggc tggagaattc
     1681 ccaagctcaa agcctaccgc cctatctgct tcccgttctc ccgcaagcag ggcacccccg
     1741 cctagccttt gcgcgacggc tctggcctcg ctgagcgtcc gtacagggat gataccagcg
     1801 caaccgtggt gcagcgccat ggtgatggtc gtcgtggctc gtatcacgtc gatcacggcc
     1861 actgctcgac cggaggcgga ccggcaggct ggatccaatc gactgaatgc cacctcgatg
     1921 atcacgatgg ttctcctggc tcagtcaccc aaccaacgcc ctgcaccaac tccattgaca
     1981 cccctctgaa gacctgctat gaacaggcgg ttatcacctt tcagcgggaa ggttccggac
     2041 acaatgcgtg cgaaattttc gtcggatcaa aagcgaacta tagccacgct gagcgtcgcc
     2101 atcgcgattc ggatgctcgg catctttctc gtacttccca tctttaccct ctatggggag
     2161 caatttacca gctcaaaacc gctgatcggc cttgccttcg gcgcctatgg gc
//
LOCUS       DATMXI010000314         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_188203, whole genome shotgun sequence.
ACCESSION   DATMXI010000314 DATMXI010000000
VERSION     DATMXI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188203"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="ruvA"
                     /locus_tag="VN444_06085"
     CDS             <1..551
                     /gene="ruvA"
                     /locus_tag="VN444_06085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWR21414.1"
                     /translation="QVFVPLSTFYQLPEIQQEVRLRIYTHVREDAIQLYGFHALEERM
                     TFELLTGVSGIGPRLAANILSGIAVEEFIPAVLEGDIARLKAIPGVGRKTAERIILEL
                     KDKVIEIPCRGRVVVDRQPSPERDRTIEDVVSALLNLGCSRKEASAAAEAAHHTVGDG
                     TDFETLVKQALKHLSEGTGKRP"
     gene            548..1603
                     /gene="ruvB"
                     /locus_tag="VN444_06090"
     CDS             548..1603
                     /gene="ruvB"
                     /locus_tag="VN444_06090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWR21415.1"
                     /translation="MTTAKQKQESGRERVANRQLQDEDQELEQSLRPKAWDDYIGQEK
                     VKANLQVFVRGAKARREPLDHLLFYGPPGLGKTSLAFMIASEMGVNIRVTSGPVIERQ
                     KDLAAILSSLREQDVLFIDEIHRLNPLVEETLYPAMEDYRLDLIIGQGPSAQSYRLKL
                     PRFTLIGATTRAGLLASPLLNRFGVVQRLDFYDETDLFRIVLRSAQILGVSIMEDGAW
                     EIARRSRGTPRIVNRLLRRVRDFAQVLGDGVITREVAQSALERLEVDTNGFDEMDRRI
                     LSTIIEKFAGGPVGIETLAVAVGEEKDTIEDVYEPFLIQQGFLARTPRGRTVTRLAFD
                     HFKLPRPAELQGDLWHG"
     gene            1648..1863
                     /locus_tag="VN444_06095"
     CDS             1648..1863
                     /locus_tag="VN444_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWR21416.1"
                     /translation="MDSLARMLILFGLVLAAIGGLLLFIGKIPFIGRLPGDIYIQRKN
                     FSFYFPLATSILLSILLTILFSLFRRR"
     gene            1898..3037
                     /gene="queA"
                     /locus_tag="VN444_06100"
     CDS             1898..3037
                     /gene="queA"
                     /locus_tag="VN444_06100"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWR21417.1"
                     /translation="MSISLRTADFNYTLPPELIAQAPVPERDRSRLLVLERNTGALHD
                     RIFRDLPEYLVPGDLLVINEAKVIPARLFGRSERRHQIEVLLLCEVEVDPDMGPAGTD
                     CWEALIKPSRQIRAGDRLTLADDTITAEVIEKRGEGRHLLKLVYDGRLADLLSRFGQM
                     PVPPYIKRQGIGYRVQGIGQTIPTSLHPTPSTLDPSTLDRERYQTVYAKHEGAVAAPT
                     AGLHFTPELIGTLTRQGIAVTPITLFVGPGTFRPVRMEEVAQHRMEPERYVIPEQTAL
                     AVKAARREGRRVIAVGTTTVRTLEHAAAMGEIRAGAGLTDLFIYPGYRFTSIDALITN
                     FHLPCSTLFMLVSAFAGRETMLAAYREAIARRYRFYSYGDAMLIV"
     gene            3098..>3256
                     /locus_tag="VN444_06105"
     CDS             3098..>3256
                     /locus_tag="VN444_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21418.1"
                     /translation="MSFELLKVDTATGARRGRLKTPHGTVETPVFMPCTCCSIDLQQL
                     ERHDFSSDA"
BASE COUNT          665 a          931 c          986 g          674 t
ORIGIN      
        1 accaggtctt tgttccgctt tcaacgttct atcagctccc ggagattcaa caggaggtgc
       61 gccttcgcat ctacacgcac gtccgcgagg atgccatcca gctttacggg tttcatgccc
      121 tcgaggagcg gatgacgttc gaactactga cgggggtctc cggaatcgga cctcgactcg
      181 cggccaacat cctgtcagga atcgcggtgg aggagttcat cccggctgtt ctggagggcg
      241 acatcgcaag gcttaaggcg attccagggg taggtcggaa aacggccgaa cggatcatcc
      301 tggagttaaa ggacaaagtc atagagatcc cctgtagagg tcgggtcgtg gtcgaccgcc
      361 agccgagccc ggaacgagat cggaccattg aggatgtcgt ctccgcgctg ctgaacctgg
      421 gctgcagtcg caaagaagcc tcggcggcgg cggaggcggc gcatcatacc gtcggcgacg
      481 ggacagactt tgaaacgttg gtcaagcagg ccctgaagca tctctccgag gggacgggga
      541 agcggccatg acaacggcga agcaaaagca ggagagcggc cgggaacggg tggccaatcg
      601 gcagcttcag gacgaggatc aggaactcga gcaaagcctc cgcccaaaag cctgggatga
      661 ctatattggt caggagaagg tgaaggcgaa tctccaggtt ttcgtccgtg gagcgaaagc
      721 gcgaagggaa cccctggacc acttgctctt ctacggtccg ccggggcttg gcaagacctc
      781 tctcgccttt atgattgcct cggagatggg ggtaaatatc cgggtcacct ccggaccggt
      841 catcgaacgg cagaaagacc tggcggccat cctctcgagt ctcagggaac aggatgttct
      901 gttcatcgac gagatccacc ggctgaatcc cctggtcgaa gagacgctct acccggccat
      961 ggaagattac cggttggacc tgatcatcgg tcagggtccg agcgcacaga gctaccggct
     1021 gaagctgccg cgttttacct tgatcggggc cacgactcgc gcagggcttc tggcctcgcc
     1081 tctgcttaat cggttcggtg tggtccagcg tctggacttt tacgatgaga ccgatctctt
     1141 caggatcgtc ctacggtcgg cacagattct aggggtatcg atcatggagg atggcgcgtg
     1201 ggaaatcgca cggcgctccc gtggaacgcc tcgcatcgtc aatcgcctgc tgcggcgcgt
     1261 cagggacttc gcccaggtgc ttggggatgg tgtcatcact cgtgaagttg cgcagagcgc
     1321 actggaacgg ctcgaggtgg acaccaatgg gttcgacgag atggatcggc ggatcctgtc
     1381 caccatcatc gaaaagtttg ccggcgggcc ggtgggaatc gagacactcg cagtcgccgt
     1441 aggagaggaa aaggatacga tcgaggacgt ctacgagccg tttttgattc agcaagggtt
     1501 tctggccaga actccacggg gccgtaccgt cactcgcctg gcctttgatc acttcaagct
     1561 cccccgcccg gccgagttgc agggcgacct atggcacggc tgacgggagc gctcaccatt
     1621 actggagaca ggggagcagt gcgggggatg gattcacttg ccaggatgtt gatcctgttc
     1681 ggcctcgtcc tggccgccat agggggactc ctcctattca tcggaaagat cccttttatt
     1741 gggaggttgc ctggggacat ctatattcaa cgaaagaact tctcattcta ttttcccctc
     1801 gcgacctcaa tactcctgag tatcctcctg accatactct tttcattatt tcgccgccga
     1861 tgagcggcga cgatgccgcc aggagctgac tcctcccgtg tccatttcgc ttcggacagc
     1921 cgactttaac tacaccctgc cccccgagct gattgcgcaa gcgcccgttc ctgagcgcga
     1981 tcgctcgagg ctcctggttc tggaacggaa cacaggcgcg ctgcacgatc gaatcttccg
     2041 cgatctcccg gagtacctcg ttccaggaga cctgttggtc attaatgagg cgaaggtgat
     2101 ccccgctagg ctcttcggcc gatccgagcg gcggcatcag atcgaggtgt tgctcctgtg
     2161 tgaggttgag gtcgatcccg acatgggccc tgccggaaca gactgctggg aggccttgat
     2221 caagccatcc aggcagattc gggccgggga tcgcctcacg ttggccgatg atacgatcac
     2281 cgccgaggtg atagaaaaac gcggtgaggg acgccatctg ctcaagcttg tgtatgacgg
     2341 aagactcgcc gacctcttat cgcgcttcgg tcagatgccg gttccacctt acatcaaaag
     2401 acagggtata gggtataggg ttcagggtat agggcaaacc attccgacat ctctacaccc
     2461 tacaccctct accctagacc ctagcacttt ggatcgcgag cggtaccaga cggtctacgc
     2521 aaagcatgaa ggggccgtcg cggcgcccac ggccggcctc cacttcacgc cggaactgat
     2581 tgggacactc acacggcaag gtatcgctgt cactccgatc acgctctttg tcggccccgg
     2641 tacgtttcgt cctgttcgaa tggaggaggt tgcacagcat cggatggagc cggagcgcta
     2701 tgtcattccg gagcagacgg ccttggcggt caaggccgcc aggagagagg ggcggagggt
     2761 gatcgcggtg ggaacgacga ccgtccggac cctggagcat gccgcagcaa tgggcgagat
     2821 acgggctggg gcaggcctga cagacctctt tatctacccg ggctatcgtt ttacgagtat
     2881 cgacgccctc attaccaact ttcatctgcc ttgctctacc ctctttatgc tggtctctgc
     2941 cttcgcaggc cgggagacga tgttagccgc gtatcgcgag gccatcgcca ggcgctaccg
     3001 tttctactcc tatggcgacg ccatgttgat tgtgtgatga ggcaaccgaa caatatccga
     3061 ctgaaggttg aaggctgata gcctcatagg agagattgtg agcttcgagt tattgaaggt
     3121 cgatactgca acaggtgcac ggcggggtcg tctgaagacg cctcatggca cggtagagac
     3181 gcctgtattc atgccgtgca cctgttgcag catcgatctt caacaactcg aacgtcacga
     3241 cttctcctct gatgcc
//
LOCUS       DATMXI010000315         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_49175, whole genome shotgun sequence.
ACCESSION   DATMXI010000315 DATMXI010000000
VERSION     DATMXI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49175"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VN444_06110"
     CDS             <1..187
                     /locus_tag="VN444_06110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWR21419.1"
                     /translation="DRLMGSRSLCRDDAMLRIRSQMPQEEKRRHAHYVIENEGLLEET
                     ERQVKAVWEQLCAKASS"
     gene            876..2123
                     /gene="rho"
                     /locus_tag="VN444_06115"
     CDS             876..2123
                     /gene="rho"
                     /locus_tag="VN444_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWR21420.1"
                     /translation="MNIVELKEKTISELSGIARILNVVGASGLRKQELIFKILEAQTE
                     KSGLIFAEGVLEVLPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFDLRTGDTVSGQVR
                     PPKEGERYFALLKVEAVNFENPELVKDKILFDNLTPLFPNQRIRLETTQDEMNMRVMD
                     LLTPIGKGQRGLIVAPPRTGKTILLQKIANSITKNHPEVVLIVLLIDERPEEVTDFQR
                     SVKAEVVSSTFDEPPTRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLGRAYNTIVP
                     PSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKG
                     TGNCELVLDRRLVDKRVFPAIDIFRSGTRKEELLLTQEELNRMWILRKVLQTMGVVEA
                     MELLLAKLRQAKSNEDFMRAMNS"
     gene            2453..2653
                     /gene="rpmE"
                     /locus_tag="VN444_06120"
                     /pseudo
     CDS             2453..2653
                     /gene="rpmE"
                     /locus_tag="VN444_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
BASE COUNT          657 a          753 c          843 g          609 t
ORIGIN      
        1 cgaccgtctg atggggtcca ggagcctctg ccgggatgac gcgatgctgc gcatcagatc
       61 gcagatgccg caggaagaga agcgtcgcca cgcccactat gttatcgaga atgaaggact
      121 gcttgaggag acggagcgtc aagtgaaggc ggtatgggaa cagttatgcg ccaaggcttc
      181 gtcgtaggcg cttgcctgtg gtttcggcag cccgcgatga gcgttgtcgc gtcaaacacg
      241 gtgcaaccca cactgagttg gatctgctac tgaaaggctt cgaaaaaagg cttgacaaag
      301 cgcatgggct ccactaggtt tatactaaca atctcgttga gggtataact tcttcagagt
      361 gtcgcttgtc ggtcggccct ggactgcgca aaattgaggt taagggcgac cggcgctgcc
      421 tgagatcaag ctcatctcac tttccgattc agaccgctgt ttcccacgca tccggcaggg
      481 gccttattcc tggtgaccgg tttctactat tcgcgcggta actactcagg tgtttagact
      541 gaaggctgaa gactgaaggt tctaagttat ctcgcaaggc acgagtcgag ccattcaaga
      601 tcttttcgga gttttgtata atcacgcatg acttacccct aaaagccttc agccttcagt
      661 ctatctacct gagttacttc acgcgcatca tactgcaaat cggtcacacg gagaggctgg
      721 tatttgattg agatcgcgaa gtagattcat cgttaacagt cttacttgag gaagaggagt
      781 tcgcgtagat ggcctcatca gtgtctgaat cgaccagacg gtccagcatg tcagaagtga
      841 aggagagcgt tgcggcgctt cccaggggtg gttcgatgaa catcgtagag ttgaaagaga
      901 agaccatctc tgagctgagc ggcattgcca ggatcctgaa tgtggtgggt gccagcggcc
      961 tccgcaagca agagctcatc tttaagatcc ttgaggcaca aaccgagaag agcgggctga
     1021 tcttcgcgga gggcgtactc gaggttctgc cggatgggtt tggcttcctt cgggcgccgg
     1081 actacaatta cctgcctggg ccggacgaca tttacgtatc gccgtcgcag attcggcgct
     1141 ttgacctgag gactggcgac accgtctcgg gacaggtgag accccccaag gaaggggagc
     1201 gttacttcgc ccttctcaag gtcgaggcgg tgaacttcga aaacccggag ctggtgaagg
     1261 acaagatcct cttcgataac ctgactcccc tcttcccgaa tcaacggatc cgactggaga
     1321 ccacacaaga cgagatgaac atgcgggtga tggatctcct cacgccgatc ggcaaggggc
     1381 agcgtgggct gatcgtcgcc ccgccgagga cgggcaagac cattcttctg caaaagatcg
     1441 ccaacagcat caccaagaat caccctgagg tggtcctgat cgtcctcctg atcgacgaac
     1501 ggccggagga ggtcacggac ttccagcgct ccgtcaaggc tgaggtggta agttccacct
     1561 tcgacgaacc gcctacgcgt cacgttcagg tggccgagat ggtgatcgag aaggccaagc
     1621 ggctggtgga acacaagcgg gacgtagtga tcttgctgga ttctatcacc cgactgggaa
     1681 gggcctacaa caccattgtc ccgccgagcg gtaaggttct ctccggcggc gttgacagca
     1741 acgcgttgca gcgtcctaaa cggttcttcg gggcggcgcg gaatatcgaa gagggcggca
     1801 gcctgacgat catagccaca gcccttatcg acaccggtag ccggatggac gacgtcatct
     1861 tcgaggagtt caaagggacc ggcaactgcg agcttgtcct ggatcgaagg ttggtggaca
     1921 agcgggtctt tcctgccatc gatatcttca ggtcaggcac acggaaggag gaactcctgc
     1981 tcacccagga ggagcttaac cggatgtgga ttcttcgcaa ggtcctccag actatgggcg
     2041 tggtggaggc gatggaactc cttcttgcga agttgaggca agccaaatcg aacgaggatt
     2101 tcatgagggc aatgaattcg taggtgattt tcccggaatc gatgcgaccc aacagcccgt
     2161 cctccgacgg gctgtatttt ttggtaacta ccaggtagat agactgtagg ctgttagact
     2221 gtaggaacgc gcacagatga gccaggcgtt ttccgaatgc gacgcgacaa tgatggagac
     2281 cggaaaggtt ccggacgcct tgattcggtc catgcgcgac tgctaacagc cttcagccta
     2341 cagcctaaac acctgggtag ttatattttt tgttgagagg ccccagtctt gggggtataa
     2401 tcagccgcgg ctaagtaact gaaaaatccg gaatatctcg ggagggacaa tcatgaaggc
     2461 aagcattcac cctgattatc aactgactac gatcacgtgc gcgtgcggcg aggtgatcca
     2521 cacccggtcc accgtactcg ctctccgggt agagatctgc tccaagtgcc acccgctctt
     2581 taccgggcgt cagaagctga tcgacaccga agggcgagtc gatcggttcc ggcggaagta
     2641 ccagaagaag ccaaaggcgg acatatctgt cccagagact gtcctgactg aacccctcgc
     2701 tgcagcgcct ggagaagcgg tctcctaaca gggagccgct cgtgtgtggg tgacgtcgat
     2761 gctgcagttg cttgaacgcc tgaagaccat cgaacagcga tcttctgaga tcgggctgca
     2821 gatgagtgat ccggctatca ttagcgatcg gccccgtttc ca
//
LOCUS       DATMXI010000316         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_29845, whole genome shotgun sequence.
ACCESSION   DATMXI010000316 DATMXI010000000
VERSION     DATMXI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29845"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..987)
                     /gene="dapA"
                     /locus_tag="VN444_06125"
     CDS             complement(115..987)
                     /gene="dapA"
                     /locus_tag="VN444_06125"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWR21421.1"
                     /translation="MFIGTLTAIVTPFRNGKLDEQAFGDLIEWQIANGTNGIVPCGTT
                     GESATLTHQEHHRVVELTVEVVNRRIPVIAGTGSNSTAEAVSLTKHAKQAGADGALII
                     TPYYNKPMQEGLYRHYKAIAESVDLPQILYNIPGRTGVNMLPSTVARLCGLQNIVGIK
                     EGSGSVQQASEIVSTCGDRITVLAGDDALTLPMMAVGGKGVISVTSNIVPSQMAQLVQ
                     AFLSSRIDEARRIHSALSPLFNALFYETNPIPVKTALGLMGKIDPELRLPLCAMATET
                     KDKLTRVLKDAGLI"
     gene            complement(1032..2294)
                     /gene="lysA"
                     /locus_tag="VN444_06130"
     CDS             complement(1032..2294)
                     /gene="lysA"
                     /locus_tag="VN444_06130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWR21422.1"
                     /translation="MHSFEYRQGELYCEQVPVSRIAKEVGTPCYIYSYETLVRHFRAY
                     DGAFKNVPHVIAFAMKANSNIAILRLMAQEGSGVDIVSGGELFRALKAGVPPSKIVFA
                     GVGKNAEEIREALKADILMFNVESSAELRALNEVAAAVGTRARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALEEFALASSLANIDVVGVHKHIGSQLTEVTPFVEAVKKVV
                     KLVEALKAQGINIRYVNIGGGLGITYSDETPPHPQDLADAVSPLLRDLNVTLIMEPGR
                     VIVGNAGILVTKVLYRKDGEAKRFIIVDAAMNDLIRPSLYGAYHEIRPLSEAVLQRPK
                     HNVDVVGPVCESGDFLAKDRSLPEVNPDDMLAVMSAGAYGFVMASNYNSRPRVPEVLI
                     KGGEIHVIKTRETYDDLVKGETIPAFLN"
     gene            complement(2287..>2816)
                     /locus_tag="VN444_06135"
     CDS             complement(2287..>2816)
                     /locus_tag="VN444_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21423.1"
                     /translation="RKLVKRTCPKGSVPPLAGVLRNLQAKLPVLDKEGLIPSALHALK
                     QHLDPNALSAQQMILHIKAKRHDKFAALFVDLDGTLDRLNALELIKRIKEAANHARMD
                     IIINFEHLKLATPDALKTLMDSDAMKVAIPNVKVRFRKFKDAFEASVQGFSLAGLQGF
                     SLAGLEHLNEDSSDA"
BASE COUNT          602 a          797 c          756 g          661 t
ORIGIN      
        1 accggacaac gtcaaggtgg tcgagtccct aattagggca accagccgac agcccatgcg
       61 accagcagcc cctgcaacga tgacgttgat catccgaacc cctcatacct cgcgttaaat
      121 gaggcctgcg tcttttaaga cccgagtaag cttgtccttt gtctcagttg ccatggcaca
      181 aagcggaaga cgcagctccg ggtcgatctt ccccatcagg cctaacgcag ttttcacagg
      241 gattggattg gtctcgtaaa atagggcgtt gaacaacggc gacaaagcag aatgaatccg
      301 ccgagcctcg tcaatacggc tgcttaaaaa tgcctggacc aattgagcca tttgggacgg
      361 gacaatgttt gacgtcacgc taatgacacc cttccctccg actgccatca tgggcaacgt
      421 gagagcatca tctcccgcca ggaccgttat ccggtcgccg cacgtactca ctatctcgga
      481 ggcttgctgg actgacccgc tcccttcttt gatcccgacg atgttctgta acccgcagag
      541 cctcgcgaca gtggacggca acatattcac gccggtacgt ccgggaatgt tatacaaaat
      601 ctgtggaagg tccacggact ccgcgattgc cttgtaatgt cggtacagtc cctcttgcat
      661 cggcttgttg taatagggag taatgatcag cgccccatcg gctcctgcct gcttcgcatg
      721 tttcgtgaga gacacggctt cggccgtgct gttggacccg gttcctgcaa tgacagggat
      781 gcgtcggttg accacctcaa cggtcagctc gactacgcgg tggtgctcct ggtgcgtgag
      841 ggtggcagat tcacctgtgg tgccgcaggg cacaatcccg ttcgtcccgt tggcgatttg
      901 ccattctatg aggtcgccga aggcttgctc gtccagtttg ccattgcgga aaggagtcac
      961 aatggcggtt aaagtaccta taaacatggg attctttccc agagggcgca ggcgtcacgc
     1021 ctctctatcg attaattgag aaatgccgga atagtttcgc ccttcaccaa atcgtcgtag
     1081 gtctctctgg tcttgatcac gtggatctct ccgcctttta tcaggacctc cggcactcgt
     1141 ggtcgcgaat tataattgga ggccatcaca aagccatagg cccccgcgct catgaccgcg
     1201 agcatatcgt ccggattgac ttccggcaat gaccggtcct tcgccagaaa atcgccggac
     1261 tcacatacag gtccgaccac atccacattg tgtttgggcc gttgcaacac tgcctcggac
     1321 aaggggcgaa tttcatggta ggccccatag aggctcggcc tgatcaggtc gttcatcgct
     1381 gcatcaacaa taatgaatcg tttcgcttct ccgtcttttc gatacaatac ctttgtcaca
     1441 aggatgccgg cattgccgac gatgacgcgg cctggctcca tgatgagagt cacattcaaa
     1501 tccctcagaa gcggcgatac ggcgtccgca aggtcttgcg gatgcggcgg cgtttcgtcc
     1561 gagtaggtaa tgccaagacc gccgccgata ttgacgtacc ggatgttgat accctgggct
     1621 tttaaggcct cgaccagctt gacgaccttc ttcacggcct cgacaaacgg cgtcacttcc
     1681 gtcaactgcg acccgatgtg cttatggacc ccgacgacat cgatgttggc aagtgaggaa
     1741 gccagcgcga attcttcgag ggcgcggtcg gcggcgattc cgaatttgct tttcttgaga
     1801 ccggtcgaga tgtagggatg cgtctttggg tcgatgtcgg gattgatccg cagggcgact
     1861 ctcgccctgg ttccgacagc agcagctact tcattcagtg cacgcagctc agctgaagat
     1921 tcgacgttga acatgaggat gtcggccttc agggcctctc gaatctcctc agcgttcttg
     1981 ccgacgcctg cgaatacgat ctttgaggga ggaaccccag ccttgagggc tcgaaagagc
     2041 tcccccccgg acacaatatc cactccgctc ccttcttgtg ccatgagacg gaggattgcg
     2101 atattggagt ttgccttcat ggcgaaggcg atcacatggg gtacgttctt gaaggctcca
     2161 tcgtaggctc gaaaatgacg gacgagggtt tcatagctgt agatatagca gggtgtcccg
     2221 acctcttttg caatccggct gaccggcacc tgttcgcaat agagttctcc ctgtcgatac
     2281 tcgaaactat gcatcagatg aatcttcgtt cagatgttcg agccctgcga gcgagaagcc
     2341 ctgcaagccc gcaagggaga aaccctgaac ggaagcctca aaggcatcct taaacttacg
     2401 aaagcgaacc ttcacattcg gaatcgcgac cttcatcgcg tctgaatcca tcaatgtctt
     2461 gagggcgtct ggcgtcgcca gcttgaggtg ttcgaagttg ataatgatgt ccatgcgtgc
     2521 atgattggcc gcttccttga tccgcttgat caattccaag gcattcagac ggtcgagggt
     2581 gccatcaagg tccacgaaca atgccgcaaa cttgtcatgc cgcttggcct tgatgtgcaa
     2641 tatcatttgt tgcgcggaga gcgcgtttgg atcgagatgc tgtttcagcg catgcagcgc
     2701 gctgggaatc aatccctctt tatccaagac cgggagcttc gcctggagat ttctcaacac
     2761 tcccgcaagc ggcggtacgg accccttggg gcaggttcgc ttgaccagtt tccgga
//
LOCUS       DATMXI010000317         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_70465, whole genome shotgun sequence.
ACCESSION   DATMXI010000317 DATMXI010000000
VERSION     DATMXI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70465"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2091)
                     /locus_tag="VN444_06140"
     CDS             complement(<1..>2091)
                     /locus_tag="VN444_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HWR21424.1"
                     /translation="ADQDLDVIRVDGVTRTNAHASIGDYVQVRKAPWRDAQKVTLAPA
                     RKGLRAVAPGEVLRQALLYRPVVRGDLISVGTASRSKEIVPPGMYPEELFRGLLGSLA
                     IGLGEVRLVVAGTVPSGIVRINPQTEVELLPEYVETKETRLPDITYDDIGGLGEVINE
                     IREVVELPLKHPELFDRLGIAPPKGVLLHGPPGTGKTLLAQALANEAKAHFATINGPE
                     IMGRFYGESEERLRAIFQEGQDNPPAIIFIDELDSIAPKREAVTGEVERRVVAQLLTL
                     MDGLMPRGNVIVIGATNRVGAIDLALRRPGRFDREIELRVPDRNGRRQILTIYTRAMP
                     LAPDVNLNWVADLTHGCVGSDLAALCREAALNALRRVLPDLDLRLETFPAEVLQRLIV
                     THEDFNQALRRIRPSALRELFIEVPRVTWDDVGGLADVKRALRESVELPLTHPQAFER
                     LGIKPPKGVLLYGPPGTGKTLLAKAVANEAKANFMLAKGSDLLSKWYGESEQRIREFF
                     AKARQVAPAIVFFDEVDALVPRRGMAAGEPHVTERIVNQLLSELDGLEELRGVVILGA
                     TNRPDLIDPALLRPGRFDALVYVPVPDAAARHEILAVHTRRMALAEDVDLKDLVRRTD
                     RFTGADLALLCMRAAQLALRKDLEAKAVTHADFLAALTETLPSVTEAMEREYAEVGKR
                     LRQAVPQQDSTLGHY"
BASE COUNT          378 a          669 c          677 g          367 t
ORIGIN      
        1 gtagtggccg agcgtggagt cctgttgtgg cacggcctga cgcaagcgtt taccgacctc
       61 ggcgtactct cgctccatcg cctcggtgac agagggcagt gtctcagtaa gcgcggccag
      121 gaagtcggcg tgcgtgaccg ccttggcctc caggtccttc cgcagggcaa gctgggcagc
      181 ccgcatgcaa agcagggcaa ggtcggcccc ggtaaatcga tcggtcctgc ggacaaggtc
      241 cttcaggtct acgtcttcgg cgagggccat gcggcgggtg tgaacagcaa ggatctcgtg
      301 gcgggcggcg gcatccggta ccggaacata caccagcgca tcgaagcgcc ctgggcgcag
      361 gagggccggg tcgatgagat caggccggtt cgtggccccc aggatgacaa ccccgcgcag
      421 ttcctccagg ccgtccagct cggacaggag ctggttcacg atccgctcgg tcacatgcgg
      481 ttcgcctgca gccatcccgc gtcgcggcac aagggcatcc acctcgtcga agaacacgat
      541 ggcgggagct acctggcggg ccttggcgaa gaactctcga atccgttgct ctgactcgcc
      601 gtaccacttg gagagcagat cgctcccttt ggccagcatg aagttagcct tcgcctcgtt
      661 ggcgacggcc ttagccagta gggtcttccc ggtccccggc ggaccataga gcaggacccc
      721 tttgggtggc ttgatgccca ggcgctcgaa ggcctgagga tgggtcagag gcagctccac
      781 agactcccgg agggccctct tcacgtcggc caggcccccg acatcgtccc aggtgacccg
      841 agggacttcg atgaacagct cccgaagcgc agaaggacgg atccgtcgca aggcctggtt
      901 gaagtcctca tgggtgacga tcaggcgctg caggacctcg gccgggaacg tttcaagcct
      961 caaatccaga tccgggagga cgcgccgtag cgcattcagg gcggcctcac gacagagggc
     1021 agccaagtct gaaccgacgc agccatgcgt gaggtctgcc acccaattca ggttcacgtc
     1081 cggggcaagg ggcatggccc gcgtgtagat cgtgaggatc tgacgccggc cattgcggtc
     1141 cggtacgcgc agctcaatct cccggtcgaa gcggccgggg cggcgtagtg ccaaatcgat
     1201 tgcgccgacg cggttcgtgg cccctatgac gatcacgttg ccgcgtggca tgaggccatc
     1261 cattaatgtc agaagctggg ccacgacccg ccgttccact tcacccgtca ccgcctcgcg
     1321 cttgggggcg atggaatcaa gctcgtcgat gaagatgatg gcgggtgggt tgtcttgccc
     1381 ctcctgaaag atggctcgca gccgttcctc tgattcgcca tagaagcgcc ccatgatctc
     1441 gggtccattg atggtggcga agtgggcttt ggcctcattg gccagggcct gagccaggag
     1501 agtcttgccg gtccccgggg ggccgtgaag gagcacaccc ttgggcggtg caatcccaag
     1561 ccgatcgaac agctctggat gcttgagcgg taactcgacg acctcccgga tctcattaat
     1621 cacctcgccc aacccgccga tgtcgtcgta ggtgatatcc gggaggcggg tctccttggt
     1681 ctccacgtac tccggcagga gctccacctc tgtttgggga ttgatgcgca caatgccgga
     1741 cgggacggtg ccggcgacga ccaggcgcac ctcgcccagc ccgatcgcca gggaccccag
     1801 caagcctcga aagagctcct ccggatacat cccgggtggg acgatctcct tcgaacgtga
     1861 ggctgtcccg accgagatca ggtcgccacg aaccacagga cggtagagga gcgcctggcg
     1921 gagaacctcg cccggggcta cggcacgcaa gccttttcgg gcgggagcca gcgtcacctt
     1981 ctgcgcatcc cgccagggag cctttcgcac ctgtacgtaa tcccctatgc tggcatgggc
     2041 attggtgcga gtcacgccgt cgacccggat cacatccagg tcttgatcgg c
//
LOCUS       DATMXI010000318         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_268234, whole genome shotgun sequence.
ACCESSION   DATMXI010000318 DATMXI010000000
VERSION     DATMXI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268234"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..376)
                     /gene="rpsL"
                     /locus_tag="VN444_06145"
     CDS             complement(5..376)
                     /gene="rpsL"
                     /locus_tag="VN444_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWR21425.1"
                     /translation="MPTINQLVRKGRQLAHAKTKSPALKSCPQKRGVCLRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DAVGVADRKQSRSKYGAKRPK"
     gene            complement(497..>3116)
                     /locus_tag="VN444_06150"
     CDS             complement(497..>3116)
                     /locus_tag="VN444_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWR21426.1"
                     /translation="CKGEGKVFGSPEEVRVAFDSRELDVHARIKVRIEGSLVQTTVGR
                     VILSDVLPPGLPFSVANKLMTKKEMTKLIDTVYRQCGHRETVTFLDKVKDTGFEYATR
                     AGISICIDNMHIPTKKQDLLGKAQHEVTEIERQYAEGLITNGERYNKVIDIWAHVTEQ
                     IANEMMKELGAGGDPTKAEAFNPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIE
                     TPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDIAQDVIINEIDC
                     GTTDGIIVSALVEGGEIIQPIEERVLGRLAAEDIRDPVTGEIIVSFNEEIDEERTKAI
                     VEAAVDHVKIRSVLTCQSRRGVCRSCYGRDLARGRLVEKGEPVGVIAAQSIGEPGTQL
                     TMRTFHIGGTASKVVEQTVTEAKHAGTIKFMSFDAKKNADFPNAGIAVQNKEGEWVVM
                     NRNAKIAIVDETGREREKYGVVYGAKIKIKNGGRVEVGQKLVEWDPYSLTILTEVGGK
                     VAYGDIIEGVSMKEEFDEVTGLSRKVIVEHSGATLRPRVSIKDEGGKTAKVAAAANVA
                     RYLLPVGAHIFVEKGQLVTPGDVLAKIPRETTKTKDITGGLPRVAELFEARKPKETAV
                     ISEIDGEVSYDGFVKGMRKVLVNNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGS
                     ANPHDILDVLGPNELQKYLVDEVQDVYRLQGVTINDKHIEIIVRQMLRKIRIEDPGDT
                     EFLPGSQISKMLFDEENERVLAKGGQPGLGKPVLLGITKAALTTDSFISAASFQETTR
                     VLTEAAINGRIDNLLGLKENVIVGRLIPAGTGFEEYRDTFVVSPKPEPVVVGQGEVAA
                     LAQENVATGLGEPARS"
BASE COUNT          639 a          982 c          744 g          751 t
ORIGIN      
        1 cttcctactt cgggcgcttc gccccatact tcgaacggct ctgctttcgg tccgccacgc
       61 caacggcatc aagagcgcct ctgacgatgt gatagcgtac tccaggcaag tccttcacgc
      121 ggccgccacg gacgagcacg atagaatgtt cttgaaggtt gtggccgaca ccggggatat
      181 aggtcgtcac ttccattcca ttcgtcagcc gaacgcgcgc gactttccgc agcgctgagt
      241 tcggcttctt tggcgtcgag gtatagacac gaagacagac tccgcgcttc tgcggacagg
      301 acttcagtgc ggggctcttc gtcttcgcgt gcgccagctg gcgacctttt ctgactaact
      361 gattaatcgt gggcattagt acttcgctct cattttcggt atagtttaac gctcttactc
      421 aacccatcaa gggcaaagcc tgaggattgt agtgaccctt ggtctctctg tcaagcctgc
      481 tccggcgccc catggatcaa gagcgcgcag gctcacccaa tcccgtcgcg acattctctt
      541 gcgcaagagc tgcaacctct ccctggccga caaccacagg ctctggcttg ggacttacaa
      601 cgaaggtatc ccgatactct tcaaagccgg tcccggccgg aatcaaacga ccgacgatca
      661 cattttcttt cagccccaac agattatcga tgcgtccgtt gatcgctgct tccgtcaaga
      721 cacgcgtggt ctcctggaat gaggccgcag aaataaagct atccgtggtc aacgccgcct
      781 tggtaatacc gagcaatacc ggcttcccaa gaccaggctg tccacccttc gccaatactc
      841 gctcattctc ttcatcaaat agcatcttac tgatttgact gccgggcagg aactccgtat
      901 cgcccggatc ttcgattctg atcttgcgca acatctgccg cacaatgatc tcgatgtgct
      961 tgtcgttgat ggtcacacct tgcagccggt agacatcctg cacctcgtcg acaagatact
     1021 tctgcaattc gttcgggccc aacacgtcga gaatgtcgtg cggattggca gatccatcca
     1081 tcaaaggctc gccagccctg acccaatcgc cctcatgaac attgacgtgc ttgcctttgg
     1141 ggatgaaata ttctttcaca tcccccattt tgttattgac cagcactttc cgcatgccct
     1201 tcacgaaacc gtcgtaggag acttcgccat cgatctcgct gatgacggcg gtttccttcg
     1261 gctttcgagc ctcgaacaat tcagccacac gaggcaaacc accggtaata tctttcgtct
     1321 tggtcgtctc acggggaatc ttggccaaga catcaccggg agtgaccagt tggcccttct
     1381 cgacgaagat gtgagccccg accggaagca gatagcgagc cacgttggcc gcagctgcaa
     1441 ccttcgccgt ctttccaccc tcatccttga tcgaaacacg cgggcgcaat gtcgcaccgc
     1501 tatgttcaac gatgacctta cgggagaggc cggtgacttc atcgaattct tccttcatgg
     1561 atacaccttc gatgatatct ccatacgcca ctttaccgcc gacctctgtg aggatggtca
     1621 aggagtaggg atcccattcg accaatttct ggcccacttc gacccggcca ccgttcttga
     1681 tcttaatttt ggcgccgtag acgaccccgt acttctcgcg ctcacgtcct gtctcatcga
     1741 caatggcgat ctttgcattc cgattcatga cgacccattc gccctcttta ttctggacag
     1801 caatcccggc gttagggaag tcggcattct tcttggcgtc gaagctcatg aacttgattg
     1861 tcccggcatg cttggcttcg gtcacagtct gctctaccac cttgctcgcg gttccaccga
     1921 tgtgaaacgt ccgcatggtc aactgtgttc ccggttcgcc gatcgactgt gcagcaatga
     1981 caccgaccgg ctcgcctttt tcgaccagcc gtccgcgggc caaatcccgc ccatagcagg
     2041 accgacagac gcctcggcgc gactgacagg tcagcacaga acgaatcttg acatgatcga
     2101 ctgcggcctc cacaatcgcc ttcgtccgct cctcgtcgat ctcctcattg aacgagacaa
     2161 taatttcacc cgtcactgga tcgcggatat cttctgcagc caaccgtccc aacacccgct
     2221 cttcgatcgg ctgaatgatt tcaccaccct cgaccaatgc actcacgatg atgccgtcgg
     2281 tcgtgccaca atcgatctcg ttgatgatca catcttgcgc gatgtccacc aatcgacgcg
     2341 tgagatagcc ggagttggcc gtcttcaacg ctgtgtcggc caatccctta cgagcgccgt
     2401 gggttgaaat gaagtattgc aacacggtca gcccttcgcg gaagttggcg gtaatcgggg
     2461 tctcgatgat ttcacctgag ggcttcgcca tcaaacctcg cataccaccc aactggcgga
     2521 tctgctgcga gcttcctctg gcgccggaat ccgccatcat gaagatcgga ttgaaagcct
     2581 cagccttggt gggatcgcca ccggcgccca attccttcat catttcattg gcaatctgct
     2641 ccgtcacatg ggcccagata tcgatcacct tgttataacg ctccccgttg gtgatcagtc
     2701 cctccgcata ttgccgttcg atctccgtca cctcgtgctg agccttaccc agcagatcct
     2761 gtttcttggt cggaatatgc atattgtcga tgcagatcga gatgccggcg cgcgtggcat
     2821 attcaaaccc tgtatccttg accttatcga ggaatgtgac cgtctcacgg tgaccgcact
     2881 ggcggtagac cgtgtcgatc agtttggtca tttccttctt ggtcatgagc ttattggcga
     2941 cggaaaaagg caaccctgga gggagcacat ccgacaggat aactcgtccg acggtggtct
     3001 gaacgaggct gccctcgatc cgcaccttga tccgagcgtg cacatccaat tctcgcgaat
     3061 cgaatgcaac acgcacttcc tccggcgaac cgaacacttt cccctctccc ttgcat
//
LOCUS       DATMXI010000319         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_118664, whole genome shotgun sequence.
ACCESSION   DATMXI010000319 DATMXI010000000
VERSION     DATMXI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118664"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(520..1275)
                     /locus_tag="VN444_06155"
     CDS             complement(520..1275)
                     /locus_tag="VN444_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21427.1"
                     /translation="MMKRVAVALAALILSLPTIAVAAPVVFEASGANPADIQATVDDF
                     RAFLGTNNGVGGSFPDGRREINWDGVPDAFSAPNNLPANFFNFNSPRGVVFFTPGTGF
                     QVSADSDNPTNTRVRFGNIHRVLPKLFSTFSPERLFTALGSNITEILFFVPGSPTGAT
                     VNGFGAVFTDVNRDDSTTIEYFDVNGALLFSENVLRGPTKRRSLSFLGVGFDAGERVF
                     LVRITSGNRILKTPDRDVVVMDDFIYGEPQALP"
     gene            1612..1953
                     /locus_tag="VN444_06160"
     CDS             1612..1953
                     /locus_tag="VN444_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21428.1"
                     /translation="MAAVCVLFAIGFLLLAPDPHDHGDAGSLGGLLRLFLPNSSQTAD
                     PHQSEDEPQSETNGSCPIHFWHQVAATGLLFALLLQFSLLSHLHIPSFVAVPYVTELG
                     SFHSRAPPVFS"
     gene            2076..>2851
                     /locus_tag="VN444_06165"
     CDS             2076..>2851
                     /locus_tag="VN444_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21429.1"
                     /translation="MCNLSTTQLGLMTITSLTALILGTGVTWAEVKNPELEALKKEVE
                     ELRRRDAEKEKRLEKLQRQIEAMRSQLPTAEKPATPQVALEEALKEIEQSAPVPVPTD
                     LVSRQVGETTFRLIDISLDALFAAGTSTKREDSIRRLQGGDHDPRKRGFTVQQVELSL
                     TGAVDPYLTGEAHIIYLIDPEVGETRVELEEAFLTTQALPYGLQLKAGHFFTEFGLIN
                     PVHPHAWDWLDQPIINTRLFGPDGLRQTGVRLGWLTPLPW"
BASE COUNT          619 a          839 c          803 g          590 t
ORIGIN      
        1 tggaggaaga cagatgagaa tcaactgtac gggatattga gggtctcccg ttgacaactg
       61 acctatttgg cattccggcg gtaaaggcgg gagctcctgt gccggcgcca cagccggaag
      121 actcacactc aacgcgaccg cacaggctaa caccaatcga atgtacgtgg ccatcaaaca
      181 cctcctgtga ctgattgaac tggcttttgt ttcggcttcc aaggtcgtgt ctggttgtca
      241 cagtcgttct cgtcgctcat gctaaaagcc catcatgccg aaggcgggac ttcacctgct
      301 gccgtcggtg caacaatgca ggaggcggaa ggagtcccag aagtccctct cgagttacat
      361 caaagcatca cagcaccgtc ttgaagggcc tctccgtgat tatcttgttt ccgcttgtct
      421 ctatcgccaa cgcctcaacc ttgtagtctg cctgcgtgag aggcgcgccg attaaggcgc
      481 gcccactcca cgcgacggtc aggcatggtt ccgcagagct cagggtaagg cctgaggttc
      541 gccgtaaatg aagtcatcca tcacgacgac gtctcgatcg ggtgttttca agatgcgatt
      601 cccgctggta atccgcacca gaaaaacacg ttcgccagca tcaaacccca cccccaggaa
      661 cgagaggctg cgccgcttcg tgggccctcg caagacattc tcgctgaaga gcagtgcgcc
      721 attgacgtcg aaatattcga tcgttgtgct atcgtcacga ttgacgtcgg tgaagaccgc
      781 gccaaacccg ttgactgttg cccccgtggg actgccaggc acaaaaaaga ggatctctgt
      841 gatgttactg cccaaagcag tgaaaagccg ctcggggctg aaggtactga agagcttggg
      901 aaggacacgg tggatattgc caaacctgac ccgagtgttc gtcgggttat cgctgtcagc
      961 gctcacctgg aaacctgtcc ccggggtaaa gaagacgaca ccgcgcggag agttgaagtt
     1021 aaagaagttg gctgggagat tgttcggggc tgaaaaggcg tctgggacac catcccaatt
     1081 gatctcccgg cggccatcgg ggaatgagcc ccccacgccg ttgttggtcc ccagaaacgc
     1141 ccggaaatca tcgaccgtgg cctggatatc ggccgggttt gcccccgatg cttcaaacac
     1201 gaccggagcg gccacagcga ttgttggtaa cgataggatt aacgcggcta aggcaacagc
     1261 aacacgcttc atcatggtgt cctcccttcg gtaacagtag cacttgatct ccgcaccccc
     1321 tcttttacag tccgagatta ctctctcggc tgaaataacc gctatcgggt aagtccttga
     1381 ctgacgggcg gaaaatccct gatggcacca cgatgacaga tgccgccggg agtttcggct
     1441 tgacacggct ggaccgcctt ggtacttggt atgagatgcc tgtagttcgg aaaccaggag
     1501 gcaccagcag tcgctccatg cctgacggca gggaagtacg cctgccagtc catgagatgg
     1561 gagatgggag acagaaacac gcaaagcgaa aaaggtcgtg gaaacagacg attggcagcg
     1621 gtctgtgtgc tcttcgctat cggattcctg ctgttggccc ccgatccaca tgaccatggg
     1681 gatgcgggct cactcggtgg gctgctgcgg ctgttccttc ccaactcgag ccagactgct
     1741 gatcctcatc agtccgaaga cgagcctcag tcggagacga acgggtcttg cccgatccat
     1801 ttctggcacc aggtcgccgc gaccggcctc cttttcgcgc ttctgctgca attctctctc
     1861 ctctcacacc tacacattcc ttcatttgtc gcagttcctt acgtcaccga actcggatcg
     1921 tttcacagcc gcgccccgcc cgttttctcc tagcatccga atcgttctga cctgcggccg
     1981 gtaagctcgc gatacccctg ccttctcggt tgccctttcg tccggggtgg ttccagctcc
     2041 ggtcggtgcc gtcgtctttt gaggaggaac catcgatgtg taatctctcc accacacaac
     2101 taggcctgat gacgatcacg tccctaactg cactgattct cggaacggga gtgacgtggg
     2161 cagaggtcaa gaacccggaa ctggaggccc tcaagaagga ggttgaagaa cttcgccggc
     2221 gcgacgccga gaaagagaag aggctggaga aactccagcg tcaaattgaa gcaatgcgat
     2281 ctcagttgcc gacagccgag aagcctgcaa ctcctcaagt agcgcttgaa gaggctttga
     2341 aggagatcga gcagtcggcg ccggtgcctg taccgactga tctcgtctcc aggcaggtcg
     2401 gcgaaaccac attccggttg atcgatatct cgctggatgc gctgttcgcc gcaggaacct
     2461 ccaccaagcg ggaggattcc atccggaggc tccagggcgg cgaccacgat cctcgcaagc
     2521 gcggctttac agtgcagcag gtagagctgt cgttaactgg tgccgtggac ccgtacctca
     2581 cgggcgaggc ccatatcatc tatctcatcg acccggaagt tggtgaaacc agagtcgagt
     2641 tggaggaagc cttcctgacc acgcaggcac tgccgtacgg actgcaactg aaagcaggac
     2701 atttctttac cgagttcggg ctgatcaatc cggtccaccc gcatgcgtgg gattggctgg
     2761 accagccgat catcaatacc cggctgtttg gacccgacgg cctgcgacag accggcgtcc
     2821 ggctaggatg gctcacgccg ctcccctggt a
//
LOCUS       DATMXI010000320         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_278233, whole genome shotgun sequence.
ACCESSION   DATMXI010000320 DATMXI010000000
VERSION     DATMXI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278233"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..289)
                     /locus_tag="VN444_06170"
     tRNA            complement(216..289)
                     /locus_tag="VN444_06170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Met,seq:cat)
     gene            complement(298..1365)
                     /gene="rlmN"
                     /locus_tag="VN444_06175"
     CDS             complement(298..1365)
                     /gene="rlmN"
                     /locus_tag="VN444_06175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWR21430.1"
                     /translation="MPNKIDLKGLSLDEIERFVTDHGEPVYRGRQLFHWIYGRGATTF
                     AEMTDLPIALRARLAELASIGALAPLGREVSQDGTRKYLFGCADGRQIETVLIPDEGR
                     MTACLSSQVGCALACAFCLTGTMGFVRHLQAAEIVDQVLALQRDLQPGERIGNLVLMG
                     MGEPLHNYDATVKALTILAHPLGLAYPPRRIALSTVGLVPEIVRLGQSGLPVNLAVSL
                     HAATDELRDRLVPINRRYPLKVLMATLRAYPLPSRRRLTFEYVLIDGVNDRPEDAREL
                     VKLLRGLRCKINLLPLNEAPAIPFHRPSRERVEAFQRILKAAGLLTTIRESRGMDISA
                     ACGLLVTASAEKRLDTAGHLA"
     gene            complement(1403..2155)
                     /locus_tag="VN444_06180"
     CDS             complement(1403..2155)
                     /locus_tag="VN444_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21431.1"
                     /translation="MMRGLAAIVLLGCFLAACATEQIAAEREKADSHYNIGIARLASG
                     DAKQAIAEFSQAIDNASDNPAYHNALGLAYLVEHRTDLAIASLQRAVELDPKFSDAYN
                     NLGSAYVQRAGYDQAIKAFRQALSNPAYLSPEQAHLNLGNIYVIQGRAADAVVEFKRA
                     LDVAPDFAEAHNRLGHVYLAQGRLELAIAELTLAIKQAPDLATAYQSLGFAHLSAGEK
                     DRARQAFQKVVELSQTSEMAAEAMRQIKQLSQ"
     gene            complement(2152..3171)
                     /gene="mltG"
                     /locus_tag="VN444_06185"
     CDS             complement(2152..3171)
                     /gene="mltG"
                     /locus_tag="VN444_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWR21432.1"
                     /translation="MSATLSSPPLPQRALAICLGLLVGGGVLWYVLGGPVPSTSEVTR
                     AVVIRPQTGAFDIARTLKEARVIRSRAAFLAVAVARGTQRRLLAGEYEFTPGLNLLEV
                     VRRLEQGKGFVHQVTIPEGFAARQIAEALQEKGLVDQDRFMGLLRDRQLLAQYRVDGE
                     SLEGYLFPDTYRLVKGLSDEAIIGRMVQRFSEVFGPAERARATEMKMSVAEVVTIASL
                     IEREAMAEEERPLISAVFHNRLRLRMPLQSDPTVLYSLSRFSGKLTRANLRAPSPYNT
                     YLHRGLPPGPIASPGRASIMAALYPASSRYLYFVSKNDRTHAFSNTLREHDAMVRRYQ
                     IRRAG"
     gene            complement(3168..3590)
                     /gene="ruvX"
                     /locus_tag="VN444_06190"
     CDS             complement(3168..3590)
                     /gene="ruvX"
                     /locus_tag="VN444_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWR21433.1"
                     /translation="MGIDFGVRRIGVAVSDELGITAQPLPSLEPSSEQEALSVIQGLI
                     DQHGVIEVVVGLPKNMNGSIGPAAEAALAFARRLGEGGAAKVTMWDERLTSRAAERLL
                     IEADLSRAKRKQRVDQVAAVLILQGFLDRYHRQQERSS"
BASE COUNT          739 a         1138 c         1053 g          665 t
ORIGIN      
        1 ctatgcgggt tcgactcccg cccccggcac caacgtatct gagtagttag ctgccccccc
       61 accgcctaca tcctagagcg catccaggat accgttcacc tacacaagat ccggaagagc
      121 ctcttactct aatcggttga aaggttgtga gcctactggg ggcatttcat aaagaatggg
      181 ctaggcgaaa ccgcctagcc cagtaaattg agaggttgcg ggggcaggat ttgaacctgc
      241 gacctttggg ttatgagccc aacgagctac cagactgctc caccccgcga agacatctta
      301 ggcaaggtgg ccagcagtgt caagcctttt ctcggcgctt gcggtgacca acaagccaca
      361 ggcggccgag atgtccatgc cgcggctctc gcggatggtg gtcaggaggc cggctgcctt
      421 caggatgcgt tggaaggcct caacgcgctc ccgcgaaggt cgatgaaacg ggatggcagg
      481 ggcctcattc aaaggcagga ggttaatctt gcaccgaagg ccgcgcagca gctttaccag
      541 ctcacgggcg tcctccggtc ggtcgttcac cccatcgatg agcacatact caaaggtgag
      601 acggcggcga gacggaagcg gatacgccct gagcgtcgcc atcaacacct tgagcgggta
      661 gcggcggttg atcgggacca gacggtcgcg aagctcatcg gtggccgcgt ggagcgacac
      721 ggccaggttc accggaagtc cactttggcc gagtcgtacg atctccggga ccaggccgac
      781 agtggagagg gcgatccgac gcggagggta cgcaaggccc agggggtgcg ccaggatcgt
      841 gagcgccttc acggtggcgt cgtagttgtg gagcggttcc cccatcccca tcaacaccag
      901 atttccgatt cgctctcctg gctgaagatc tcgctggagc gcgagcacct gatcgacgat
      961 ctcagccgcc tgcaagtgcc ggacaaaacc cattgttcca gtcagacaaa aggcgcaggc
     1021 cagggcacac cctacctggc tggagagaca cgcagtcatc cgcccctcat cagggatcag
     1081 taccgtctcg atctgtcgcc cgtccgcgca gccaaacagg tatttccgcg taccatcctg
     1141 ggagacctct ctgccgagag gcgcgagggc gccaatgctc gccagctcgg ccagcctcgc
     1201 gcgtaacgcg atcggcagat cggtcatctc cgcgaaggta gttgcgccgc gcccgtagat
     1261 ccagtggaag agctggcggc cacggtagac cggctcgccg tggtcggtaa caaagcgctc
     1321 tatctcgtca aggctcagcc ctttgaggtc aattttgttg ggcacttttt cactcaatag
     1381 ataggaaaga ggtgcgttga ggctattggc taagctgttt gatctgacgc atcgcctcgg
     1441 ccgccatctc gctcgtctgg ctcagttcca ccaccttctg aaaagcctgc ctggcccggt
     1501 ctttctcccc agccgacaga tgggcaaagc ccagactctg atatgcagtg gcgagatctg
     1561 gcgcctgttt tatggccagt gtcagttcgg cgatcgccag ctccaaccgt ccttgcgcca
     1621 ggtagacgtg tcccagccga ttatgggcct cggcgaaatc gggggctacg tccagagcac
     1681 gtttgaactc cacaaccgcg tcggcagccc gaccctggat gacgtagata ttccccagat
     1741 tcaggtgcgc ctgctcgggg gacaggtagg cggggtttga aagggcctgc ctgaaggcct
     1801 tgatggcctg gtcatacccg gcccgctgga cataagccga accgagatta ttgtaggcat
     1861 cggagaattt cggatcgagc tcgacagccc gttgcaagga ggcaatggcc agatcggtcc
     1921 gatgctcgac cagataggcg agtcctaagg cgttgtgata ggccggattg tctgaggcgt
     1981 tgtcaatcgc ctgactgaac tctgcgatgg cctgcttggc gtcaccactg gccaggcgcg
     2041 cgatgccaat attataatga ctgtcagcct tttcccgctc cgccgcgatc tgctcggtcg
     2101 cgcaggcagc gagaaaacag cccagcagaa cgatggcggc cagaccacgc atcatcccgc
     2161 tctcctgatc tgataccgcc tcaccatcgc atcgtgttcc cgcagcgtat tcgagaaggc
     2221 atgcgtcctg tcattcttcg acacgaagta cagatatcgg gaggaggcgg gatacagggc
     2281 ggccatgata gaggcacgtc cagggcttgc gatcggaccg ggcggcaggc cccggtgaag
     2341 atacgtgtta tacggggagg gcgcccgcag gttcgctcta gtgagcttac cgctaaaccg
     2401 cgagaggctg tagagcaccg taggatcgga ttgcagcggc atcctcagtc gtagcctatt
     2461 atggaacacc gctgagatga gcggccgttc ttcttccgcc atcgcttccc gttcgatcag
     2521 ggaagcgatg gtcaccacct ccgcaactga catcttcatc tccgtggcgc gcgcccgttc
     2581 cgcaggaccg aatacctcag aaaatcgctg aaccatccgc ccgataatcg cctcatcgct
     2641 cagtcccttg acgaggcgat aggtatcggg gaacagatac ccttcgagcg actcaccatc
     2701 cactctgtac tgcgccagca actgacggtc ccgcaggaga cccataaacc gatcctgatc
     2761 gaccagcccc ttctcctgaa gcgcctcggc aatctgtcgg gcggcgaacc cctctggaat
     2821 cgttacctga tggacgaacc ctttcccctg ctcgagccgg cggacaacct caagcaggtt
     2881 gaggccgggg gtaaactcat actctccagc aagcaggcgc cgttgcgtac cacgggcgac
     2941 agccacagcc aggaaggcgg cacgactgcg aatcacacgc gcctctttca acgtcctcgc
     3001 gatatcgaag gctcccgtct gcggcctgat gacaacggcc cgcgtgacct cgctcgtgga
     3061 cggcactggc ccgcccagga cgtaccacaa gacaccccct cccaccagga gaccgaggca
     3121 gatggcaaga gcccgctgcg gcagcggagg tgaggagagt gttgcactca cgaggagcgc
     3181 tcctgctgac gatggtaacg atcaaggaaa ccctgcagaa taaggacggc ggccacctga
     3241 tcgacgcgct gcttccgttt ggctcgcgat agatcggcct cgatcagcaa gcgctcggcc
     3301 gccctactgg tcagccgctc gtcccacatc gtaacctttg cagccccacc ttcccccagt
     3361 cgcctggcaa acgccagggc cgcctcagcc gcagggccga tggagccgtt catgttcttt
     3421 ggcaggccga cgaccacctc gatcacacca tgctggtcaa tcagcccttg aatgacgctg
     3481 agggcctcct gctctgaaga gggttcgagt gatggcagag gctgggccgt gatccccaac
     3541 tcatcactta cggccacccc gatccgccgc accccaaaat cgatccccaa gtatc
//
LOCUS       DATMXI010000321         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_182889, whole genome shotgun sequence.
ACCESSION   DATMXI010000321 DATMXI010000000
VERSION     DATMXI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182889"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..631)
                     /gene="scpB"
                     /locus_tag="VN444_06195"
     CDS             complement(38..631)
                     /gene="scpB"
                     /locus_tag="VN444_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWR21434.1"
                     /translation="MDTPADLGPIGVLEALLFVSEAPLSLERMEEMLDGCSRAEVSRL
                     LADLQEKYRQPDRGLVVTEVAGGYRLATKPEVAPWVQRLRGSKPARLSRAALETLALI
                     AYKQPITKIEIEAIRGVMVDGVVKTLVERDLIRILGRKPEVGRPILYGTSRSFLEYFG
                     FKDLSELPTMKEIEALTPSTTEKEASDEAVDQTEKAE"
     gene            complement(615..1352)
                     /locus_tag="VN444_06200"
     CDS             complement(615..1352)
                     /locus_tag="VN444_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWR21435.1"
                     /translation="MSYRVRLEMFEGPLDLLLYLIQVNEIDIYDIPIAKITQEYLGCL
                     AGMEDLDLEIAGEFLVLAATLIHIKSKMLIPVQESVTEGESADDPRQELVERLLEYRR
                     FKEAAMKFEELEAGQALLYVRPADTAVPIAEGPLEVSLSALLRAFTAIMQRAPEARAV
                     EITLDTINVGERMVAVLDRLTLQSPVSFLALFDGVTTRALLVATFLGLLELLRRGLVR
                     ARQSEPGSEITIYRTVEAVVGADGHPG"
     gene            complement(1356..1757)
                     /locus_tag="VN444_06205"
     CDS             complement(1356..1757)
                     /locus_tag="VN444_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWR21436.1"
                     /translation="MKGTYTRREAIAAGLFGVAVAGIVWGPVMLRPFGSAPIIDLRTL
                     QLQQDRPAAQASLSHPTRVTKVKPTSRVDINHADAVTLQTLPGIGPTLAQRIITHRKG
                     YGRFTDADGLREVEGIGSRRFEKLEPWIEAR"
     gene            complement(1754..2992)
                     /locus_tag="VN444_06210"
     CDS             complement(1754..2992)
                     /locus_tag="VN444_06210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWR21437.1"
                     /translation="MSLIVQKYGGSSVADVERIKNVARRVVETKLHGHDLVVVLSAMA
                     GETDRLLGLAAKVSDTPDERELDVIVTTGEQISIGLLSLAIQQRGHKARSFTGAQVRI
                     QTDTAHTRAKIVSIEADRVQQALRDGAIVIVAGFQGVTAEEDVTTLGRGGSDLTAVAM
                     AAALKADLCEIYTDVEGVYTADPNVVPEARKLGKISYDEMLEMASLGAKVLQARAVEY
                     AKNYGVPVHVRSSFNSNQGTLVVQEDAEMERVVVSGIACDRNEAKITVLRVADRPGIA
                     AKLFGRVAEANIVVDMIVQNISQDGTTDISFTVPKSDFPKAMRLLNGVAQEIGAQQVE
                     GDDRIAKVSIVGVGMRTHSGVAARMFEALARENINITMISTSEIKISCLIDAKYGELA
                     VRILHETFGLAERNVVEERT"
BASE COUNT          607 a         1048 c          810 g          650 t
ORIGIN      
        1 atatcgctgc agccgttcct ggtcaccatc agtcgactca ttctgccttc tcggtttgat
       61 caactgcctc gtctgacgcc tccttttcgg tcgtactcgg agtcagtgcc tcgatctcct
      121 tcatggtcgg cagctccgag aggtccttaa atccaaagta ttctaagaag gatctgctgg
      181 tcccgtagag gatcggcctg cccacctctg gctttcgtcc gagaatccgg atgagatccc
      241 gctccacgag cgtcttgacg actccgtcca ccatgacccc gcggatcgcc tcgatctcta
      301 tcttagtaat cggctgctta taggcaataa gtgcaagcgt ctcaagtgcg gccctggaca
      361 gtctggccgg tttggaccca cgaagccgct ggacccacgg ggccacctcg ggttttgtcg
      421 ccaggcgata gcccccggcc acctcggtca cgacaagtcc gcggtccggt tgccggtatt
      481 tttcctgtag gtcggcgagc aaccggctta cctctgcccg tgagcacccg tcgagcatct
      541 cctccatccg ttccagcgat agtggcgcct cggagacaaa caacaaagcc tcaagaactc
      601 caatcgggcc gagatcagcc ggggtgtcca tcggccccca ccaccgcttc cacggttcga
      661 tagatcgtga tctcgctccc cggctccgat tgcctggctc tgaccaggcc ccggcgcaac
      721 agctcgagca gcccgagaaa ggtcgcgacc aataaggcgc gcgtcgtgac cccatcgaac
      781 agggccaaga acgataccgg actctgaagg gtcagccgat ccaacaccgc caccattcgc
      841 tctcccacat tgatcgtgtc cagggtgatt tcgaccgccc tcgcctctgg cgcgcgctgc
      901 atgatcgccg taaatgcgcg aagcagcgct gacaggctga cctccagagg accctcggcg
      961 atcggaaccg cggtatcggc aggcctcaca tagagcagtg cctgccccgc ttccaactcc
     1021 tcgaacttca tggccgcctc cttgaatcgc ctgtactcca ggagacgttc taccagctcc
     1081 tgccgcgggt cgtccgcaga ctctccctcg gttaccgact cctgcaccgg gataagcatc
     1141 ttcgacttga tatggatcag cgtggcagcc agcaccagaa actctcctgc gatctccaga
     1201 tcaagatcct ccatcccggc caagcatccc aggtattcct gggtaatctt ggcaatgggg
     1261 atgtcgtaga tgtcgatctc gttgacctga atcagataga gcaagagatc cagggggccc
     1321 tcaaacatct ccaggcgaac acgatagctc atcgcttagc gcgcctcgat ccagggctca
     1381 agcttctcga atcgcctcga accgatccct tcaacttccc gaagcccgtc agcatctgta
     1441 aaccggccat agcccttccg atgggtgatg atccgctggg ccagtgtcgg accgatccct
     1501 ggcagcgtct gaagcgttac ggcatccgca tggttaatgt ccacccggct ggttggtttt
     1561 acctttgtga ccctggtcgg atgcgacaga gaggcctgcg ccgcgggccg gtcttgctga
     1621 agctgcagcg ttcgaagatc aatgatagga gcagaaccga acggacgaag catgacagga
     1681 ccccacacaa tgccggcgac agccacacca aagaggccgg cagcgatcgc ctctcgcctg
     1741 gtgtacgtcc ccttcacgta cgctcctcga ccacgttgcg ctcggccagg ccgaaggtct
     1801 cgtgcaaaat ccggacagcc agctctccgt acttggcatc gatcagacac gagatcttga
     1861 tctcggaggt gctaatcatt gtgatgttaa tgttctcgcg tgccagggcc tcaaacatcc
     1921 tcgcagccac cccggagtga gtccgcatcc ccacccccac gatggagacc ttggcgatcc
     1981 tgtcgtcacc ttcaacctgc tgagcgccga tctcctgagc caccccgttt aggagccgca
     2041 tcgccttggg aaagtccgac ttcgggaccg tgaacgagat atcggtcgtc ccatcctgac
     2101 tgatgttttg aacgatcata tccactacaa tgttggcctc cgcaacccgg ccgaacagct
     2161 tggctgcgat ccccggacga tccgccacgc gcagcacagt aatcttcgcc tcgtttctgt
     2221 cgcaggcaat cccagagacc actaccctct ccatctccgc atcctcctgg accaccagtg
     2281 tcccctggtt tgaattaaaa ctggaacgga cgtgaaccgg cacgccgtaa ttcttggcat
     2341 actctaccgc tcgggcttga agcaccttcg cccccaaact cgccatctcg agcatctcat
     2401 cataggagat cttcccgagc ttcctcgcct ctggaacaac attcggatca gcggtgtaga
     2461 ccccctccac atcggtatag atctcgcaaa ggtcggcctt cagagccgcc gccatggcca
     2521 ccgctgtcag gtctgatccg ccacgcccca gcgtcgtcac gtcctcttcc gccgtcaccc
     2581 cctggaaccc ggcgacgatg acgatggctc catcccgaag cgcctgttgt acccggtcag
     2641 cctcgatgct cacaatcttt gccctggtgt gggcggtgtc ggtctggatc ctgacctggg
     2701 ctccggtaaa agagcgggcc ttgtgcccgc gctgttggat tgccagcgac aggagaccga
     2761 tcgagatctg ttccccggtg gtcacgatga cgtcaagctc gcgttcatcc ggagtatcgg
     2821 agaccttggc ggcaaggcct aacaggcggt cggtctctcc agccatggct gagagcacca
     2881 cgaccaagtc gtgcccatgg agcttcgtct ccaccactcg acgcgcgacg tttttgattc
     2941 gttcgacatc tgctacagag cttccgccgt atttctgaac gatgagagac atgtcttgtc
     3001 cttacgaggc gagccgatcg ggccgaggca gatcagctcc ccgcagtttt tgtccgatct
     3061 tcccctcttg ccccaccata aggcgtctca gattttcccg gtgccggacg aggat
//
LOCUS       DATMXI010000322         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_268324, whole genome shotgun sequence.
ACCESSION   DATMXI010000322 DATMXI010000000
VERSION     DATMXI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268324"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..105
                     /locus_tag="VN444_06215"
     CDS             10..105
                     /locus_tag="VN444_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21438.1"
                     /translation="MVKACRPVWAKVTGTFTARGGLSSKIEAKYP"
     gene            133..954
                     /locus_tag="VN444_06220"
     CDS             133..954
                     /locus_tag="VN444_06220"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWR21439.1"
                     /translation="MATYAIGDVQGCSDALQRLIQHIRFDPSVDRLWFVGDLVNRGPD
                     SLNVLRYIKNLRDRAVVVLGNHDLFLLAVAEGIVTVRPEDTLQGILAAPDRDELLAWV
                     RQQRLLYREGLFVLVHAGLLPQWTIDESEKLAREVEAGLRGPAYRDILRALYPSKHLQ
                     WSSSLNGPTRLATIVKVMTRLRACSPDGRMESSFNGPPDLIPSGYFPWFKIEDRKHRD
                     TTIICGHWAALGLHCEEHLLTIDSGCVWGRQLTAMRLEDRTVFQVDCNNVGCRGD"
     gene            947..1447
                     /locus_tag="VN444_06225"
     CDS             947..1447
                     /locus_tag="VN444_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWR21440.1"
                     /translation="MTEPEPPDPHWPRYSTRPFPPYHFVPGRAPHPRRDPRGHSYGLP
                     EPKPTPFPADKWHTSEDYLYGIDLYNFAYWWECHEVFEGLWHAVDHTSEQGNFFQALI
                     QVAAANLKSFLGNSAAARNLLRSGIIRLQKVPPSYLGIDVPRLTEALQAHLISAHPHA
                     PLIALG"
     gene            complement(1722..>2411)
                     /gene="panB"
                     /locus_tag="VN444_06230"
     CDS             complement(1722..>2411)
                     /gene="panB"
                     /locus_tag="VN444_06230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWR21441.1"
                     /translation="GIETILVGDSLGVVVQGKANTISVTMEDMLYHTKLVAGAALQAL
                     VIGDMPFMSYQASREEAVRNAGRFLQVGAHAVKLEGGAAVADQVAAMTSIGIPVMGHL
                     GMTPQSVHRYGGYKVQGRDSDHAQALLNDAKVLEAAGAFALVLEAIPAELAKIVTEQV
                     AIPTIGIGAGPHCDGQVLVLYDLLGLFDDFVPKFVKPYAHLKADALQALRRYKEEVEQ
                     GKFPSDSESYH"
BASE COUNT          533 a          712 c          636 g          530 t
ORIGIN      
        1 ctccacgata ttgtcaaagc ctgccggcct gtctgggcga aagtcaccgg cacctttacc
       61 gcacgcggcg ggctgtcgag caagatcgaa gcgaagtatc cataatcggc atttcctgtc
      121 ttactctgag gtatggccac ctacgcaatc ggcgatgtgc aaggatgttc cgatgccttg
      181 caacgcctca tccaacatat tcgatttgac ccttccgtag atcgtctttg gttcgtcgga
      241 gatctcgtca accggggtcc ggattcactg aacgtcctcc gctacatcaa gaatcttcga
      301 gatcgagctg ttgtcgtatt gggtaaccac gaccttttcc ttcttgccgt ggccgagggt
      361 attgtcacgg tgcgtccgga agacacgcta caaggaatac tggccgcgcc agatcgcgac
      421 gagctcctgg cgtgggtacg gcaacagcgg ctcttgtatc gcgagggtct ttttgtcctt
      481 gtccacgcag gtttgctccc tcagtggacc atcgatgagt cagagaagct ggcgcgtgaa
      541 gttgaagctg gcttgcgagg tcctgcgtat cgagacatcc ttcgcgcgtt gtaccccagt
      601 aaacatctcc aatggtcttc aagcctgaat ggtcccactc ggctcgcgac catcgtcaaa
      661 gtcatgacaa gactccgagc ctgttcaccc gatggtcgga tggaatcgtc atttaatgga
      721 cctccggacc tgatcccctc tggatacttc ccttggttca agattgagga tcgaaaacat
      781 cgcgacacaa cgatcatctg cggacattgg gccgcactag ggcttcattg tgaggagcat
      841 ctcctcacga tcgacagcgg ctgtgtatgg gggcgacagc tcacggccat gcgactggaa
      901 gaccggacgg tatttcaagt cgactgcaac aacgtcgggt gtcgtggtga ctgagcctga
      961 accgccagac ccgcactggc ctcgctactc aacccgtcca ttccccccat accatttcgt
     1021 gcccggaagg gccccacatc cacgccgtga tccacgcgga cattcctatg gactgccgga
     1081 gccgaaacca acgccattcc ccgccgacaa atggcacact tctgaagact atctctatgg
     1141 aattgatctc tacaatttcg cctactggtg ggaatgtcac gaggtcttcg aagggctgtg
     1201 gcacgcagtg gatcacacca gcgagcaggg aaactttttc caagccctga ttcaagtcgc
     1261 cgccgcaaat ctcaaatctt ttctcggtaa ctccgcggca gcacgtaacc ttcttcgcag
     1321 tgggatcatt cgattgcaga aggttcctcc ttcgtatcta ggcatcgacg tgcctcgcct
     1381 cacagaagcg ttacaggcgc acttgatcag cgcccaccca cacgctccac tcatcgcctt
     1441 aggctaaccg cactaggacc aggctttggg aaagcaaggc cgcagcgtct gaagaaggct
     1501 gaggacgagg ttaaggttga gcgttggtaa gctttctgag cagcctgcta gaacttcgat
     1561 gatccgcacc tgtggaaaga ttaggagaat gctcctgcag gctgctcaaa aaggctatcc
     1621 agcgaggccg caggcgagaa agaaccggag acgtaccctc ggggtacgtt gaggattgtt
     1681 tcgagccgag aacgaagttg ggagcctttt tcagcagcct gctagtggta ggactcggag
     1741 tcgctgggaa acttcccctg ctcgacttct tccttgtagc gtcgaagggc ttggagcgca
     1801 tccgccttca ggtgggcata gggcttcaca aactttggca cgaaatcatc gaataatcca
     1861 agaagatcat agagcacaag aacctggcca tcacaatgcg gcccggcgcc gattccaatg
     1921 gtaggaatag ccacctgttc tgtaacaatc ttagccagct cggcagggat cgcttcgagc
     1981 acaagagcaa acgcgccggc tgcttcaaga accttggcat cattcagcaa tgcctgcgca
     2041 tggtccgagt ctctcccttg cactttgtag ccgccatatc ggtgcacaga ttggggagtc
     2101 atgccgaggt gccccatgac gggaatgccg atactcgtca ttgcagccac ctgatcggct
     2161 acagccgcgc cgccttccaa cttgaccgcg tgagccccga cttgcagaaa ccgtcccgca
     2221 ttgcggaccg cctcttcccg actggcttga taggacataa acggcatatc accgatgacg
     2281 agtgcttgca gggcagctcc tgccacaagt ttggtgtgat acagcatgtc ctccatcgtc
     2341 accgagatcg tattggcctt cccctgcacg acgactccca gtgaatcacc gaccaggatc
     2401 gtctcaatac c
//
LOCUS       DATMXI010000323         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_49307, whole genome shotgun sequence.
ACCESSION   DATMXI010000323 DATMXI010000000
VERSION     DATMXI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49307"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VN444_06235"
     CDS             complement(<1..145)
                     /locus_tag="VN444_06235"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWR21442.1"
                     /translation="MIERRKTRQIQVGPVSVGGNAPVSVQSMTKTDTRDVRATVDQIW
                     ALEA"
     gene            complement(260..1390)
                     /gene="rseP"
                     /locus_tag="VN444_06240"
     CDS             complement(260..1390)
                     /gene="rseP"
                     /locus_tag="VN444_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWR21443.1"
                     /translation="MIASVSTVAFSLLDVVDPRPFLSRLDSVLWAVLVLGGLIFVHEL
                     GHFLVAKRAGVRVLKFSLGFGPKIIGFTRGETEYLLSAIPLGGYVKMHGEDPKEEVSD
                     PEGSFSAKSVGWRSLIILAGPGSNLLLAIVIFWVVFTIGVPTLATKVGEVMEGFPAHE
                     AGVLTGDRIKAIDGRPIEKWEELATQIHQSPGRPIRLTVERAGDRFDLVVAPKATRQK
                     NLFGEEQEIGLLGIAPAEEFLTERTNPATAFGRAIYKTYDLSRLILLTFVKLIQGVVP
                     AKTIGGPLLVAQMAGQQAHLGVLPLLFFTALLSINLAILNLLPIPILDGGHLLFSLIE
                     AFRGKPVSLQKREMAQQVGMALLVALMIFAFYNDIFRLLGKQ"
     gene            complement(1418..2572)
                     /locus_tag="VN444_06245"
     CDS             complement(1418..2572)
                     /locus_tag="VN444_06245"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWR21444.1"
                     /translation="MKRVSILGSTGTIGVKALAMIDLHRDSFEVVALAARDNIDLLEQ
                     QIRRFSPRVVAVGTSKGATILKDRVKDVPVEIGWGDEGVLNVATASEADIVLTAIVGA
                     AGLLPSLAAIKAGKDVALATKEVMVMAGELVIAEARARGIRLLPVDSEHSAIFQCLGG
                     QQSCAYLKRVLLTSSGGPFRQRPKESFASITPKEALQHPTWIMGKKITIDSATLMNKG
                     LEVIEASWFFSLTPQQIDVIIHPQSIIHSMVEFMDGAILAQMGATDMGLPILYALSYP
                     DRLQSPLPPLDLNALSALTFEPVDHEKFPCLGFAYQALQAGGTYPAVLNAANEVAVDL
                     FLSGRVTFPDISALIAKTMDSHRGRKIDSLEDALDADREARRLVLAALQA"
     gene            complement(3569..3937)
                     /locus_tag="VN444_06250"
     CDS             complement(3569..3937)
                     /locus_tag="VN444_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWR21445.1"
                     /translation="MRTGQVIASAQEELAAAKRTRKRRIVFVAAVLVILIAAPSVGGK
                     KSLVKVFQMSKTRTELQREINRLRQVNEEIAREIQTLAHNPSQVEAIAREDLGLVKPG
                     EIVYQFDPQRLTTALPAPSR"
BASE COUNT          900 a         1191 c         1123 g          822 t
ORIGIN      
        1 ccgcctccaa tgcccagatc tgatccaccg tggctcggac gtcccgcgta tcggtcttcg
       61 tcatcgattg gacagacacc ggcgcgttac cgccgacact aactggtcca acctgaatct
      121 gccgtgtttt acgtctttca atcatggttc ctcagaaaat agctgtcagt gatcagcact
      181 cagccgtcag cttcagacaa aaccgggggc atatcagaaa agctgagagc cgaccgctga
      241 aagctgattg ctgatttgct cactgttttc caagcaggcg gaagatgtcg ttgtagaagg
      301 caaaaatcat caaggccacc aacagggcca ttccaacctg ctgagccatc tctcgtttct
      361 gcaggctgac tggtttaccg cgaaaggctt caatcagcga gaagagcagg tgccctccat
      421 ccaggatagg gatcggtagc agattcagga tcgccaggtt aatggagaga agggcggtaa
      481 agaacagaag aggcaagaca cccaggtgcg cctgctggcc ggccatctgc gccaccagga
      541 gcggtccgcc aatcgtcttg gccgggacaa cgccctggat cagcttgaca aaggtgagca
      601 ggatcaggcg actgagatcg tacgtcttgt agatcgctct tccaaatgcg gtggctggat
      661 tggttcgctc cgtcaggaac tcttctgccg gcgcgattcc aagtagaccg atctcctgtt
      721 cctccccgaa gaggttcttc tggcgagtgg cctttggagc caccaccaga tcaaaccggt
      781 ctcccgctcg ttcgacggtt aggcgaatag gccgtccagg gctctgatga atctgggttg
      841 ccaactcctc ccacttctct atggggcgcc cgtcgatcgc cttgattcga tcgccggtca
      901 gcacgccggc ctcgtgcgcc ggaaagccct ccatcacctc ccccaccttg gtagcgaggg
      961 tcgggacgcc gatcgtaaag acaacccaga agataacgat agcaaggagg agattcgatc
     1021 cggggccagc caggatgatc agcgagcgcc acccgaccga ttttgcggaa aacgatcctt
     1081 ccgggtcgga cacctcttcc tttggatcct caccgtgcat cttgacatag ccgccaagcg
     1141 ggatggcgga tagaagatat tcggtctcgc cacgcgtgaa cccgatgatc tttgggccga
     1201 aaccgaggga aaacttcagg accctgaccc ccgcccgctt ggcgaccagg aagtggccga
     1261 gctcatggac gaagatgagc ccgcccagga cgaggacagc ccaaaggaca gagtcgagac
     1321 gcgaaaggaa cggtcgagga tcgaccacat ccaagaggct gaaggcaacc gtagataccg
     1381 aagctatcat gccagcgtac aagcccttct cctcatctta cgcctgcagt gccgccagga
     1441 ccagccgccg ggcctcgcga tcggcatcca gggcatcttc aagagaatcg atcttgcgac
     1501 cccggtggct atccatcgtc ttggcgatga gggcggaaat gtcagggaag gtgacccggc
     1561 cggaaagaaa caggtctacc gccacttcgt tggctgcgtt gagaacggcc ggataggtcc
     1621 cccctgcctg aagcgcctga taggcgaatc cgaggcaggg gaatttttcg tggtcaaccg
     1681 gttcgaaggt taaagccgac agggcgttta agtcgagggg cggcagtgga gactgaagcc
     1741 ggtcagggta tgagagggca tacaggatcg gcagccccat gtccgtcgcc cccatctgag
     1801 ccaggatcgc cccgtccatg aactctacca tcgaatggat gatgctctgc gggtgaataa
     1861 tcacgtcgat ctgttggggt gtaagggaaa agaaccaact ggcctcgatg acttccagcc
     1921 ccttattcat gagggtagcc gaatcgatgg taatcttctt ccccatgatc catgtcgggt
     1981 gctgcagagc ctctttcgga gtaatgctcg cgaaactttc tttcggtcgc tggcggaacg
     2041 gtccgccaga ggaagtcaag agtacccgct tcaaatacgc gcagctctgt tgtcccccca
     2101 gacactggaa gatggccgaa tgttcactat cgacgggaag gagtcgaatg ccccgcgccc
     2161 gggcctcggc gatcaccagc tcccccgcca tgaccatgac ctctttcgtg gcgagggcaa
     2221 cgtcctttcc cgctttgata gcggcgaggc tcgggagcag gcccgccgca cccactattg
     2281 cggtgaggac gatgtcggcc tctgacgccg tggcgacatt cagcaccccc tcgtctcccc
     2341 atccgatctc aacgggtacg tcttttactc gatccttcag gattgtcgcg cccttagagg
     2401 ttccgacagc gacaacgcgg ggagagaacc gcctgatctg ctgttccaga agatcgatat
     2461 tatccctagc cgcaagggcc accacctcaa aggagtcgcg gtgcagatcg atcattgcca
     2521 gggccttgac cccgatagtc cctgttgaac caagaatgct tacgcgcttc actgtcgctt
     2581 ctccacttgg cgctacacca tgctaatcat caacagtagg cccactatcc gctggagttt
     2641 aagatcctag tgtagtcgga tcgggaacct ttggcaagag cttttggcac accgaagggc
     2701 cagttattag acgtgagtgg cgctcccccc ctggagtaga ctggagtaga agagtggtcg
     2761 caaccaattt ctcgcaggaa tgccaatgca tacgagcaaa gcaacgcaga gaagcagctt
     2821 aggaggcggc agatcgctcg tcaggtagag gctggcgccg ttctaacgtg atgcgtcact
     2881 actcaggtgt ttaggctgta ggctgaaggc tgttagtcat tgcgcatgga ccgactcaag
     2941 gcacccagaa ccttttcggt ctccatcact atttgcggcg cattcggaaa cgcctggctc
     3001 atccgtgcgc gttcctacag tctaaaagac tacagtctat ttccctgggt agttacggaa
     3061 cggcgctgat tcttcaatgc cgagacacct ggttggacag agaggaccga aagccctcct
     3121 gcaggccttc gatcctctct tacgtgcgag acgaggcgga agattcgagt ggggcagcgt
     3181 tatttccgtg gctaatcctt aaagacccac cgagcgaaaa aaataagaca tgcaagccca
     3241 ataagccctc cgatagcccc catcggcgtc ataaggatat tcaaccatac ctgagccatt
     3301 cctgccatct gccatcttct cctttatatt atgcgagtgt caactgtgta gcgcagcaag
     3361 gatccgcctc ggtagccgag caccgcgcta ccagggagat acatctctga ggctcctaac
     3421 cgttacccca actacacacc atacgctagg ggtatagctt cacaaaactg ccgagctttg
     3481 tcaagcgcaa aagtcaggtc cgaatataat acggccttat cgtgatcgaa ggtacgtgga
     3541 gagatttgaa gaaggatggc actgcacctc aacgggaggg agccggcagg gcagtggtga
     3601 gcctctgcgg atcgaactgg taaacgatct cgcccggctt gaccagcccg agatcctcgc
     3661 gagcaatcgc ctcgacctga ctcggattgt gggcgagggt ctggatctcc cgagcgatct
     3721 cttcgttgac ctgcctgagc cgattgatct cccgctgaag ttcggtcctg gtcttgctca
     3781 tctgaaagac cttgacgagg ctcttcttcc cgcccacaga gggagctgcg atcagaatca
     3841 ccagaacggc ggcgacgaag acgatccgcc gtttgcgggt gcgcttcgct gctgcgagct
     3901 cctcttgcgc gctggcgata acctgtcccg ttcgcatcct tactccacca cccgctcaga
     3961 cattgagtag cgcccgtgcc gctgtcgtca actcctcgat ctgcgcctcg gaatgcgcgt
     4021 ccaggtagag acgcac
//
LOCUS       DATMXI010000324         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_71576, whole genome shotgun sequence.
ACCESSION   DATMXI010000324 DATMXI010000000
VERSION     DATMXI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71576"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VN444_06255"
     CDS             complement(<1..1009)
                     /locus_tag="VN444_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21446.1"
                     /translation="MFRVGYRQAGPLMLRIRGSVILLTFALVVLLVGGGLLYLRTRMA
                     AEQLRHFAERMLARQLNLPVQIGSVSLSLLRTSVDLRQITVGDLSTTTPVQAGQRVDL
                     PLLTVDQARVTFRRSSLLRGALQIRSLIIHGPRLAVTDSPASSSILAKFVSGLSEISD
                     DQEAEGFPILLEQGAVAYQKTTSPLGLQLNGLQGRIEWPSPDQAVVTMTADDMMVRFG
                     AYKLQKIGLQAHARLTTDGVQVEQLSLAKDGSLLTLTGIVRAGADRPQVELNVAGQID
                     PAALASRLGGTVPWGRHLTVKGKIVAETTLQAVNTSLLLEDGSGRLVGQTDTMIQNGL
                     LT"
     gene            1350..1505
                     /locus_tag="VN444_06260"
     CDS             1350..1505
                     /locus_tag="VN444_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21447.1"
                     /translation="MDSSGTVRDKARVHVGVAASNEAGVADNPVNDERDIRIVGLHVE
                     APDGGVS"
     gene            1734..1892
                     /locus_tag="VN444_06265"
     CDS             1734..1892
                     /locus_tag="VN444_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21448.1"
                     /translation="MTPVRPALAGLVGEVSTGPSANAPSVITSTPMPTHRLVRRSDQP
                     VEEQLRQP"
     gene            complement(1983..3122)
                     /locus_tag="VN444_06270"
     CDS             complement(1983..3122)
                     /locus_tag="VN444_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21449.1"
                     /translation="MKRTIVTLSFATQLILSLMFVLSPLLWAPAVMAHELWVLTPEQA
                     MEWNARPKPEIFTQLNVTSLSIYLLAALFLVGCFLLYRYTASRPLASRLHARLIAYGD
                     YAPVALRIALFIMLGTASLGLAPRLGTGIMQSPVLGAPDLELRLLPGNWSWVAVLEGV
                     VAVCLLLGIYVRGTAVALLGLGLLGLFLFGEAMCAYIGLVGAAAVYLLFQGAGPYAVP
                     LPSFPGTARLSAWFAGQPRERVQCLLRLLVGADVLYLGLAYKFAQPNLMLGVLDLHHV
                     PTLGMEPETFVLVVAIVETLAGAVILAGIMIRPVAVGLFVAFVFFSVVLWEGVFSHIV
                     VYGLLVPLITNGAGQWHASLKTKVILTPGSWTFKAGRYGVVRIES"
     gene            3886..4323
                     /gene="tnpA"
                     /locus_tag="VN444_06275"
     CDS             3886..4323
                     /gene="tnpA"
                     /locus_tag="VN444_06275"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HWR21450.1"
                     /translation="MEIRLGAHGAYHHQYHLVWIPKYRKRVLTGALKDCLAKQLGEIR
                     EYHLDVEVEQVSIQVDHVHLVAVIPPKYAVSAIVGKIKANTSREIRQRFPWIKKVYWR
                     NEFWSVGFFSSTVGSDEAVIKRYVEFQEKMDTEQFTLQLDFEL"
     gene            complement(4594..>4860)
                     /locus_tag="VN444_06280"
     CDS             complement(4594..>4860)
                     /locus_tag="VN444_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21451.1"
                     /translation="KQQIAAVSRAPGNLDLFVIGFDNHVWTTFWPDPAAGGHWHQEWF
                     PIPGQHVFDRDKQQIAAVSRAPGNLDLFVIGFDNHVWSAYWTPV"
BASE COUNT         1031 a         1389 c         1499 g          941 t
ORIGIN      
        1 ctgtaagcag cccgttctga atcatcgtat ccgtctgtcc cacaaggcgt ccgctgccgt
       61 cctcaagcag aaggcttgtg ttgaccgctt gaagagtcgt ctcggcaacg atcttcccct
      121 ttacggtaag atgtctaccc caagggaccg tgccgccaag tcgcgatgcg agcgctgcgg
      181 ggtcgatctg tcctgcgacg ttcaattcca cctgtggacg atctgcacct gcacgaacga
      241 tgcccgtcaa ggtgagcaat gagccatcct tcgccagact gagctgttcc acctgcacac
      301 cgtcggtggt gaggcgcgcg tgggcttgca ggccaatctt ttgcagtttg taagccccaa
      361 accgtaccat catgtcgtca gcggtcattg tgaccaccgc ctgatcggga gagggccact
      421 cgatccgacc ctggagaccg ttcaactgga gaccaagcgg ggatgtcgtc ttctggtacg
      481 cgacagctcc ctgctccagt aagatcggga acccttctgc ctcctgatca tctgagatct
      541 cgctgaggcc ggagacaaac tttgcgagga tcgatgacga tgcgggactg tctgttactg
      601 ccagccgagg accatggatg atcagagagc gtatctggag cgcaccacgt aggagagaac
      661 ttcgtcggaa tgtcacacgg gcctgatcga ccgtcaggag cggtagatcg actcgttgac
      721 ctgcctgtac cggggttgtg gtcgacaggt cgccgaccgt gatctgtcgg aggtccacgc
      781 tagtgcggag aagcgacagc gacacacttc caatctgtac gggaaggttc agttgacggg
      841 cgagcatccg ctccgcaaaa tgacggagtt gctctgcagc catccgcgtc cggaggtaca
      901 gcaggccgcc gcccacgagc agaacgacga gagcaaaggt aaggaggatt accgatccac
      961 gaattcgcaa catcagcggt cctgcttgcc gatacccgac gcggaacacc gagcaggctc
     1021 ccatgcgatg caggggcgca gtcggccgcg cccctcgtct ttacgtgcca aggttcaacg
     1081 gcgaagcatc tcacgattgt cgctttcaat caagcttcgc ggtgatggcc aattcgtgcg
     1141 gttggcaaag agctctggac agctgctttg gcaggtgatc ccggtctccg cgactaacca
     1201 gctttcgatt accgagaccc agaggcggag agtgtgctcc tgcgtctcat cgatcctgac
     1261 gggtagggta gtatcgttac caggagaaat cagatgcggg ccgcctctta cgggtgaggt
     1321 gacctctgag gagtgggtga agtggttaca tggacagctc cggaacggtc cgtgacaagg
     1381 ccagggtcca cgtcggtgtc gctgcctcca atgaggccgg ggtagcggac aacccggtca
     1441 atgatgagcg ggatattcgg atcgtaggcc ttcacgtgga ggccccagac ggtggtgtct
     1501 cctgatggct gcagggtgta ggttacggcg accgccaccc gtcgtccatc ccattcctga
     1561 tttacaccgt actctcccgt ccccggacgt cgtcgctgac atgagaagtc atccgccctc
     1621 ggttctcgag aagagggaga ggggactgcg tacggactat ttgcgctgtt tgccgtcgcg
     1681 cgccctctgg cctccgtgcg gacgagcgcg gcgccggcgg cggggtagct gggatgacac
     1741 cagtccgacc ggcgctcgca ggcctcgtgg gggaggtgtc caccggccca agcgccaacg
     1801 ctccgagcgt catcaccagc accccgatgc cgacccatcg gctggtgcgt cgctccgacc
     1861 agccggttga ggagcaactg cgacagccgt gatccgtcgc actgctcacc aacgtggcct
     1921 cgtctctcct ctcaggttgt ctgcgatgcg gcatcacgtt acctcacgcg gatcaagatc
     1981 tttcaagatt cgattctcac aaccccatat cgccctgctt tgaaggtcca tgacccagga
     2041 gttaatatga ctttcgtctt tagggacgcg tgccattgcc cggctccgtt ggtgatcaac
     2101 gggaccagca gcccgtacac gacgatatga gagaatactc cctcccaaag gaccacgctg
     2161 aaaaacacaa acgcaacgaa gagcccgacg gccacgggtc gaatcatgat cccggcaagg
     2221 atgacggccc ctgcgagcgt ttccactatg gccaccacga ggacaaaggt ctccggctcc
     2281 atccccaggg tagggacgtg gtgaagatcg agtacgccca gcatgaggtt aggttgcgcg
     2341 aatttatatg cgagtccaag gtagagtacg tcggcaccaa ccaagagccg cagcaggcac
     2401 tgtacccgct cgcggggctg gcctgcgaac caggcgctga gcctggcggt gcccggaaaa
     2461 gacggaagcg gcaccgcata gggaccagct ccctggaaca acaagtagac cgcagccgcg
     2521 ccaaccagcc cgatataggc gcacatggcc tcgccaaaga gaaagagccc cagtagtccg
     2581 aggccaagaa gggcgacggc tgtacccctc acatagatac ccagcaacag acagacggcg
     2641 acgacgcctt cgaggacggc tacccaactc caattgccag ggagcaagcg caactccagg
     2701 tccggtgcgc ccagcaccgg gctctgcatg attccagtgc ccagtcgcgg cgccagccca
     2761 aggcttgccg tccccagcat gatgaagagc gcaatgcgca acgccaccgg cgcatagtcg
     2821 ccatacgcga tgaggcgagc gtgcaggcgt gaggcgagcg ggcgcgatgc ggtatatcgg
     2881 taaagcagga agcagccgac caggaagagg gccgccagca ggtagatgga cagactggtt
     2941 acgttgagct gagtgaagat ctcgggcttg ggtctggcgt tccattccat tgcttgctct
     3001 ggtgtcaaga cccagagttc atgggccatg acggcaggtg cccacaagag cggcgagagc
     3061 acgaacatga gagacaggat caattgggtg gcaaagctca gggtgacgat tgttctcttc
     3121 atttcacagc ctccttttat taagagaaat ctctatcacg acaaccgttc ttgataacaa
     3181 aaggcatgcc aaaacggaca tctcgcagta ggtttgtcta actcgttgtt ttcattgtat
     3241 tcgccagaca ggctgcgctc gcggaaggag aggatcagat acaggaggca ggtttcaaat
     3301 gaaacagcat gtttgagtcg gtttcatact gaaacgccga cagaccgtgg gcgtcagccg
     3361 ttaatctggt gggtttctat cgatgggaag ggcactgaaa ggaatattcc atgagcgcca
     3421 agagtcccgc tcctggtgta cgccccgggc atctcctccg gaacgccatc ccgcagacgc
     3481 gctaaccgcg ccagctaccg gctctgctgg caggccacgg gtgtgccctc agcgaagctt
     3541 cccaccagga tgctgacaat cttgacttac tatgagcatg cgggcgattc atcgaggtgt
     3601 actagttcga tgaaggtatg gagtcgagct gcaggccagc cgtgagagaa ggactgatct
     3661 gagatgcgtg agaacatgcc taagggtccg aatcagacct tttaagttgg gcgtgtcggg
     3721 ggtagataaa gagcagcggc aggtgagata ccaggcctgt acctcacctg ccgcattttt
     3781 ctatcacttt tgaggctgag gaccccgggc gttagcccgg gggtgaaagc ctcccgccga
     3841 aggcggggca ggttcgggga ctcggttctc tgatatgatg ggcgcatgga gatacggctg
     3901 ggcgctcatg gagcctacca tcaccagtac cacctggtgt ggattcccaa gtaccggaag
     3961 cgggtgctta cgggggcgct caaagactgt ctggcgaagc agttgggaga gatccgggag
     4021 taccatcttg atgtcgaggt ggagcaggtc agtatccaag tggaccacgt ccacctggtg
     4081 gcagtgatcc cgccgaaata cgcggtgtcg gcgatcgttg ggaagatcaa agcgaacaca
     4141 agccgtgaaa tccggcagcg cttcccgtgg atcaagaagg tgtactggcg gaacgagttc
     4201 tggtccgtcg ggttcttctc gtccaccgtc ggcagcgacg aagcggtcat caaacggtac
     4261 gttgagtttc aggagaagat ggacaccgag caattcacgc tccagttgga tttcgagctc
     4321 tgatctccag tgccacgggc gtaagcccgt gggtatccac cgacccaggc atcgtgatca
     4381 ggttcttcgt gtgaatctgg ggggttcatt ccccgcggct tggcgcgcag gtttcttttc
     4441 gttctggcgc tcgatgcccc gcagcttgct gcgtggtaat tcacttcacc ggcctgcgcg
     4501 cttttcgcgc aggcgcaggc cggtggaatg ataggttagg tagttgctgt ggacaacgag
     4561 aagaagttgc ccaccctgtc tcgctgtgta ttattagact ggagtccaat aggccgacca
     4621 gacgtggttg tcgaagccga tgacgaacag gtccagattg ccgggagccc gggagaccgc
     4681 ggcgatctgt tgcttgtcgc ggtcgaatac atgctgcccg ggaatcggga accactcctg
     4741 gtgccagtgg ccgccggcag cgggatcggg ccagaacgtg gtccagacgt ggttgtcgaa
     4801 gccgatgacg aacaggtcca gattgccggg agcccgcgag accgcggcga tctgttgctt
//
LOCUS       DATMXI010000325         5104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_133796, whole genome shotgun sequence.
ACCESSION   DATMXI010000325 DATMXI010000000
VERSION     DATMXI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133796"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(489..628)
                     /locus_tag="VN444_06285"
                     /pseudo
     CDS             complement(489..628)
                     /locus_tag="VN444_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011748252.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            737..1033
                     /locus_tag="VN444_06290"
     CDS             737..1033
                     /locus_tag="VN444_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21452.1"
                     /translation="MKAARLTREKAQAKSQELAVKRERLVHQAAAAEQEHRRRLRHLE
                     ERLEQKRKEREVLAPVSGEIVGRWEVPQDQFIKVHLALRTGSNDRPAEERREEK"
     gene            1069..1524
                     /locus_tag="VN444_06295"
     CDS             1069..1524
                     /locus_tag="VN444_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21453.1"
                     /translation="MAVALCFAMGTAALGAAEVRQGGSAPPPEPGPADLMAEGALRYP
                     CPSLGELEAKLLNNYEVRMMKLEAEEARLKSSLLNRIRLSASTGLSARDTLLPTEAGG
                     AISGGLHAGLSLTASIPLAELLLRGSPSSIESRIAGLLGEPYEFPALGR"
     gene            1527..1736
                     /locus_tag="VN444_06300"
     CDS             1527..1736
                     /locus_tag="VN444_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21454.1"
                     /translation="MAIDPSVLKGVRAKVLKSFMGEDGHVHIMLKDGACLRIRDACDA
                     CLRLPAKEEAFDHCSLHRARPHRPA"
     gene            1776..2051
                     /locus_tag="VN444_06305"
     CDS             1776..2051
                     /locus_tag="VN444_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWR21455.1"
                     /translation="MTKTELAEQMAQDAGISKAVAETALRSCIDSITKSLKNGQEVTI
                     VGFGTFSVASRAARTGRNPKTGEPITIKASRVPRFKPGKALKDAVSR"
     gene            complement(2136..2510)
                     /locus_tag="VN444_06310"
     CDS             complement(2136..2510)
                     /locus_tag="VN444_06310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWR21456.1"
                     /translation="MRRRWALLEADLGHPLGHEQGFLRPVLVISNEAFNQASGLLTVL
                     PATSLKPDRQPHPFEVFLPAGAAGNPLDSILLPHQIRTIAAHHIHRLCGRLTDRQLRH
                     EVAEKVLRHLEFLDLLGLDEEP"
     gene            complement(2516..2794)
                     /locus_tag="VN444_06315"
     CDS             complement(2516..2794)
                     /locus_tag="VN444_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWR21457.1"
                     /translation="MPIVAKRMTFRLHQPTVESIDELVKGGLAPSKAALIERLVEQAL
                     RRYQQQQREAEAFRQYQAAFGDPCYRIEQDAIAEDFARADAEAAAQIV"
     gene            3133..3555
                     /locus_tag="VN444_06320"
                     /pseudo
     CDS             3133..3555
                     /locus_tag="VN444_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(3839..4198)
                     /locus_tag="VN444_06325"
     CDS             complement(3839..4198)
                     /locus_tag="VN444_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWR21458.1"
                     /translation="MTHLERIRKEISPARRKKIETRAAQLIAEEMTLQELRRARKLTQ
                     VRMAKELGIGQDGVSKLEKRADLMISTLRKTVEAMGGRLSLVAEFPDRPPVALSGIGE
                     DEPDPKPAGRKQVHAHA"
     gene            complement(4278..4577)
                     /locus_tag="VN444_06330"
     CDS             complement(4278..4577)
                     /locus_tag="VN444_06330"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR21459.1"
                     /translation="MKWEADLHDDFVAEYRGLHTDVQDELLAHIELLEQFGPQLGRPH
                     TDTLKGSRHTHMKELRFDAADGAWRVAFAFDPYRKAIILAAGDKSGVSEKRFYRH"
     gene            4641..4904
                     /locus_tag="VN444_06335"
     CDS             4641..4904
                     /locus_tag="VN444_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21460.1"
                     /translation="MGDRLINDLRTEILPGFTYTGMLAFPAAPNTYAAATISFFDITT
                     KVNSVGTPTEKTQLDFLLRPQQAQMWFDRQERMWKTGATPAVP"
BASE COUNT         1001 a         1429 c         1608 g         1066 t
ORIGIN      
        1 gttccagatc cgcttaagaa aggttgacac acggcgccag gcgatctcct ggcgaaggcg
       61 gccggaggga cctgccagat agttcctgta tgcaacagcc tgtttcccga aggcggcgaa
      121 cgatctgttc atcacaacct caggtctgtg agcttccata cgtaacacag tattcacctg
      181 ttcatcaacc agttttttcg cccaagaagg tgggaaacgg gagcttttcc gctatgccgg
      241 cgagcgaggg aggatgtggt gaggggattt cgtgtgcaga ggcgagggcg cggccttttc
      301 gagggtgttg tgacactcag gagtgagcgt gttattcgaa gaggtcgatg agtataggat
      361 gctcaggtct ggcaggagcg gtgtgctgag ggtgtcgctc gcctcgtggc tcagtttggc
      421 gacgcggccg tagccctggg gtgtctccgg tgggtatcta caaagccccg tttacgaaaa
      481 agagagtcgc ggccgttcag gagcgcggac agcgcgggcc tggtgacacc gagcccttgg
      541 ccgccgcggt catggacagg ccaatggagt caagaaattc catcttgatg aactgccccg
      601 gatgggcggg gtgtgcgagg cgtagcatgg tgtgttctcc tttcagtggt agtcctcgta
      661 atcgagatcg agaatttcga aagaactatt ggcttggcag ccccttcagc gtgcggcggc
      721 tttctttcca aaggagttga aggcggctag actcacacga gagaaggccc aggccaagag
      781 tcaggagctt gcggtgaaac gggagaggtt ggttcaccag gcggccgccg cggagcagga
      841 acaccggcgg aggctacggc acctcgagga gcggctagag cagaagcgga aggagcggga
      901 ggtcttggcg ccggtctccg gagaaatcgt cggtcgttgg gaggtcccgc aggaccagtt
      961 catcaaggtc cacctggcgt tgaggaccgg atcgaatgat cgtccggccg aggagagaag
     1021 agaggagaag tgatcgttac acatcgaacc ggcagggtgt gaatccgagt ggctgtggcg
     1081 ctctgcttcg ccatgggcac cgccgccctt ggcgccgctg aggtaaggca gggtgggtcc
     1141 gcacctcccc ctgagcccgg gccggcggac cttatggcgg agggggcgct tcgctatccc
     1201 tgtccttctc ttggtgagct agaggcgaag ctcttgaata attacgaggt gcggatgatg
     1261 aaactggagg ctgaggaggc gaggctcaag tcgagcctgc tgaaccggat caggctatcg
     1321 gcctcgacag ggctatctgc ccgtgatacg ctgctgccca ccgaggccgg gggcgcgatc
     1381 tccggcggcc ttcacgctgg cttgagcctc accgcttcca tccccctcgc agagcttctg
     1441 ctccggggat ccccctcatc gatcgagagt cgaattgctg ggttgctggg agagccatac
     1501 gagtttcccg cactggggag gtaagagtgg ctattgatcc gagcgtgctg aaaggggtga
     1561 gggcgaaggt cttgaagtcg tttatggggg aggatggcca cgtacacatc atgctgaagg
     1621 atggcgcctg tctcaggatc agagatgcct gcgatgcgtg cctccgcttg cctgccaaag
     1681 aggaagcctt cgatcattgc tcactgcatc gtgcgaggcc ccatcgtcca gcctgatcga
     1741 ggtcgtcgcg ttcactatca caacaggagg aggacatgac caagaccgag cttgcagaac
     1801 agatggccca ggatgccggt attagtaagg ccgtcgctga aactgcactt cgcagttgta
     1861 tcgacagcat caccaaaagc ttgaagaatg ggcaggaggt gacgatagtg ggattcggca
     1921 ccttcagcgt ggcgagccga gccgcccgga caggccgtaa ccccaagacg ggagagccga
     1981 tcacgatcaa ggcgtctcgg gtgccacgct tcaagccggg aaaagccctg aaggatgctg
     2041 tgagccgcta aagggcgttc aggcgcgttt cgggaagggg gcgcgtggtt cggccagcct
     2101 actcgcctcc tcttgactag atgcggccgc cacacctagg gttcttcgtc cagtcccaga
     2161 agatcgagaa actccaaatg acgcaggacc ttctcggcca cctcgtgacg cagttggcgg
     2221 tccgttaagc ggccacagag gcggtggatg tgatgagcgg cgatcgtccg gatctggtgc
     2281 ggcaggagaa tcgaatccag agggtttcca gcagctcccg ccggaaggaa cacctcgaaa
     2341 gggtgcggct ggcgatccgg cttgagtgag gtggcaggaa ggacggttag aagaccagag
     2401 gcctgattga aggcctcatt actgatcaca agcaccggtc ggaggaagcc ctgctcgtga
     2461 ccgagggggt gccccaggtc cgcttccagc aaggcccatc gtcgccgcat cgcggttaca
     2521 cgatttgtgc ggcggcttcg gcatccgccc tggcgaagtc ctcggcgatc gcatcctgct
     2581 caattcgata gcaaggatcg ccaaaggccg cctgatactg tctgaatgcc tcggcctcgc
     2641 gttgttgctg ttgatagcgc cggagggctt gctccacgag ccgttcgatg agtgcggctt
     2701 tgcttggagc cagaccgccc ttgacgagtt cgtcgatgct ctccacggtc ggctggtgaa
     2761 ggcgaaatgt cattcgttta gcaacaatag gcatatccat gtcctcctgc caccaatata
     2821 gcacgctagg atggcgtgtc aagcgtgaac gtcataaata ggttcggaaa caagacgaag
     2881 cgctgtatgt cagcctgtac tgtccgttct cagagagtct acgatcgcgt cgtgaacgtt
     2941 gactgaagcg gtgctttgcc cttgcccgca cacgggagag ctattatact ttcggctaat
     3001 gcgcctgtcc tacacagccc tcaaaacctt tcagcaatgc cgtttcgcct tccggctgcg
     3061 gtatcattat ggcctgccca gctccttact cgttactgag aggcgcatgg cgggcggttt
     3121 ctacggccgc tcctcgtcga ggcgccgttt gccttccggg tcggaccctt tcggttgagg
     3181 gggcacttcg atcgggtgga cgagctcgaa gatggtcatg agatgatcga ttacaagctc
     3241 tcgcgtcgcg ggctgctgcc gcccgatccg ctgcagctcc atctctatca gctaggcctg
     3301 cacgcggaga ccggttgcac ggcgagccgg ttatcgttct atgatcttcg ctcgaaccgc
     3361 aagaacacca tcgaggcctg cgactcagag gaatcgaccg cccggattcg agggctgtgc
     3421 cgtgctatta gcgtcgaacg taggttcgag ccgagcgagg gggtctggtg cgaactgaag
     3481 atctttcgag ttggagagcc ggtgctacgt cgaaagacga ttcgtctggc ggccataggc
     3541 agatccacgt cctagaccca ctccgccttg acctgacgcc cgacggcgcg agccgtgcgg
     3601 gtcagtgtct ccaacccctc ttccttcagg aactcatcga acgtagagcc gacgttccct
     3661 tctgtcggtt gcgcttgcgc atcactcgat ctctctcatc ctcctgcggg ccaggagcgt
     3721 gcgacgggcg tattcggtca agcagatagt gcagatggtg ttgtatgaga cagtcagtgg
     3781 tggtcctcgt catcgatgtt gaggatgacc gaagagccat cggatccgcc cgtcccccct
     3841 acgcatgtgc gtgcacctgt ttccgccctg ctggtttggg gtccggctcg tcttcgccga
     3901 tgccggacag agcgaccggc gggcgatcag ggaattccgc gacaagagac agcctgccgc
     3961 ccatcgcttc aacggtcttc cgcaatgtgg aaatcatcag atcagcgcgt ttttcgagct
     4021 tggacacgcc gtcctgcccg atgccgagtt ccttggccat gcggacctgc gtgagcttgc
     4081 gtgcgcgccg gagttcctgc aacgtcattt cctccgcgat gagctgggcc gcgcgggtct
     4141 caatcttctt acgacgggcc gggcttattt ccttccgtat tcgttcaagg tgcgtcacgg
     4201 tcctgacctc ctttcctttt ccttctgcgt tttgactgtg gccagatgcg cgtcaaaacg
     4261 tgcatcagcc ttatcgatca gtgacggtag aaccgctttt cgctcacgcc tgacttgtct
     4321 ccggctgcca gaataatagc cttacggtat gggtcaaacg cgaaggccac acgccaggca
     4381 ccgtcagcgg cgtcgaaccg cagttccttc atatgtgtgt ggcgtgagcc cttcagggtg
     4441 tccgtatggg gccgcccgag ctgcgggccg aactgttcaa gcagttcgat gtgggccagt
     4501 agttcgtcct gcacgtccgt atgcaggccg cggtactcgg cgacgaaatc gtcgtggaga
     4561 tcagcttccc acttcaccca tgcagtatgc actataataa ctatgtcgtc aagtgcatgt
     4621 acggtggaca gagtatcttc atgggtgacc gacttatcaa cgatctgagg acggagattc
     4681 tcccggggtt tacgtataca ggcatgctgg cgttcccggc ggctcccaat acctatgcgg
     4741 cggccacaat ctcattcttc gacatcacga caaaggtgaa ctcggtgggg actccgacgg
     4801 aaaagacgca gctcgacttt ttgctgcggc cccaacaggc tcagatgtgg ttcgaccggc
     4861 aggaaaggat gtggaagact ggagcaaccc ccgcggtccc ctgagtggat cggctgaaac
     4921 aagcttcggg gacatgacac gcattcctgc gtgactccgg ggcatctgga tttgggaaag
     4981 gcagacgatc tcctcaaagg agtcgcaaat cagtatgatg tcccgggagt catgatgtac
     5041 ccggattcgc gaatccgtcc aaaacccttt gaaagcacgg gaggctccct agcggtgcct
     5101 ggct
//
LOCUS       DATMXI010000326         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_248336, whole genome shotgun sequence.
ACCESSION   DATMXI010000326 DATMXI010000000
VERSION     DATMXI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248336"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VN444_06340"
     CDS             <1..627
                     /locus_tag="VN444_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21461.1"
                     /translation="EKEVWRRQAKKPTVADGPTCKENITAVVEQIAPRLFKFLEKQCR
                     GLGLQNVFDQLTGEFAEAKVKFIKEADREIDIWIGCAAIEPERPPIPAVPIILNFQGY
                     GIFQTGTHSQAIVAPSIAKESARIIEDLSHARTEIAELKELQAEQKEELIGALNAAIT
                     EVSKEHPNRLTVGGIITSVASATSILADSTKAYETVKGIARQFGVTLP"
     gene            complement(830..1519)
                     /locus_tag="VN444_06345"
     CDS             complement(830..1519)
                     /locus_tag="VN444_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWR21462.1"
                     /translation="MVIGAFLAACLLPSVEAHEIPEWEETYIQGVFSPKFTPPAAGTY
                     DLPVIKHVPAFVLIDATGRRVNTGSLTHGKVAVVSFIYTACSDRLGCPLAGMALKELQ
                     VQLLREGLQDRAVLLSISLDPTRDTPKQLAKYGHIFGVESSLWHLLTAPSEQALRTAL
                     ESYGQDRVKIYDENGRFTGRYRHVLKVFLVDQSGQVRNIYSTGSLVPQVMVNDIKTVL
                     AEKAAGRGQLR"
     gene            complement(1711..2961)
                     /locus_tag="VN444_06350"
     CDS             complement(1711..2961)
                     /locus_tag="VN444_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HWR21463.1"
                     /translation="MRPYHRPVGWLWLMAITLMAGPAVADETCQSPFLPKVTGQEDYV
                     YVWTLGIEGVGDGNDSLVTVDVNPKSKAYGQIIRRAPVPGRHEAHHAGFTDDRRFLWA
                     GGLDDSSIFIFDVASDPARPKLVKTITSFVKDTGGLVGPHTFYALPGRMLISALSNAK
                     DQSGRTGLAEYSNEGRFIRTIWMPKEAPYGYDVRVNVNLNRMLTSSFTGKKNYMRPLG
                     ELVKDAEAMKTFGGTVVVWDFHARKPLQVLQVPGAPLELRWALMPNHYYAFTATALAH
                     QLVLIYQQADGTWAAKSIADLGDTLPVDISIAPDDSKLYVTSFMDGMLRVYDISNPFE
                     GKLIEQVKVGEMANMVSESWDGTRLYVTNSLLSKWDKPGDYWLKAYAWENGKLVHKFT
                     TDFNAVGRAHLMNFGSKALRIKVE"
     gene            3735..4082
                     /locus_tag="VN444_06355"
     CDS             3735..4082
                     /locus_tag="VN444_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21464.1"
                     /translation="MRSVVAVGLAVLVMASSTPNWAEAEAQESTLSQVGTGVGSAIGT
                     FVYFPFKAAFCILGGVASGFTVVFAGPENAGKVARTACGGSWVITPDVVKGKETVKFA
                     GDPAPSRQRPSAK"
     gene            complement(4139..4702)
                     /locus_tag="VN444_06360"
                     /pseudo
     CDS             complement(4139..4702)
                     /locus_tag="VN444_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007548258.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            5445..5933
                     /locus_tag="VN444_06365"
     CDS             5445..5933
                     /locus_tag="VN444_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21465.1"
                     /translation="MSGQRWNPWKLTTIGIAVVFATALITGLVVANWVGNQQTKPVAE
                     VPRGRVSRAVSPRMTPSPAARPSASAVAACNQYASSMAGDRTTEVVKDALVGGALGAG
                     VGAAGGAIAGGGKGAGKGAGIGGLVGATAGTLYGLSEARQTDARYVAAYRGCMRSRGY
                     SG"
     gene            6292..>6765
                     /locus_tag="VN444_06370"
     CDS             6292..>6765
                     /locus_tag="VN444_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21466.1"
                     /translation="MPMIDEETELNVFERVAEYQPAPSWRTWLFGRPLQSKEPRSRNA
                     GMTLIWMSAVLGTLFLGITFLAVKVGAIPAEQETVISQLVRTVFQGQGLLYLATIAGT
                     TLILVMAANTAFADFPRLSALQAGDGFLPRQLTYRGSRLVFSRGIVALALIASLLI"
BASE COUNT         1403 a         1995 c         2003 g         1364 t
ORIGIN      
        1 gaaaaggaag tgtggcgtcg ccaggccaag aaacccacag tggctgatgg accgacgtgt
       61 aaggagaata ttacggcagt cgtagagcag atcgccccgc gactgttcaa gttcctggaa
      121 aagcaatgcc gagggctcgg gcttcaaaat gttttcgatc agcttacagg cgagtttgcg
      181 gaggcaaagg taaagttcat aaaagaagca gatcgtgaga tcgacatctg gattgggtgc
      241 gcggcgattg aacctgaacg gccacccatt ccagcagttc cgattatcct caacttccaa
      301 ggatacggaa ttttccagac cgggacacat tcgcaggcta tcgtcgcgcc ctcgattgct
      361 aaagagagtg ctaggatcat tgaggatctg tcccatgcga ggactgagat tgccgagttg
      421 aaagagttgc aagctgaaca aaaagaggaa ctcattggag cgctcaacgc ggctatcaca
      481 gaggtttcaa aagagcaccc gaaccggctc accgtgggag gcatcataac tagcgttgcc
      541 tcagctacca gtatcctcgc agattcgact aaggcctatg aaacggtcaa gggtatcgcg
      601 aggcagttcg gtgttacact cccgtagaat cagaatccac ggtaaggacc aaaataccgc
      661 gactcatcgt tgttgcccgc cagatctccg tcaaagtgag tcccgcggag aagccccttc
      721 ccgatgggga taaggcccct cactcacgtg aagcgcggca ccggcgatta ccgctgaggg
      781 aaagctggcc cgcccagggt agaggaggat tcgttctctc tgaccgcgct taccggagct
      841 gtccgcgacc agcggccttc tcagccagca ccgtcttgat gtcgttcacc atgacctgtg
      901 gcaccaggga gccagtgctg tagatgttgc ggacttgccc tgactggtcc accaggaaca
      961 ccttgaggac atgacggtat cggccggtga aacgaccgtt ctcatcgtag atcttcaccc
     1021 gatcttgccc gtaactttcc agcgctgtcc ggagcgcctg ttctgaaggc gccgtcagca
     1081 gatgccagag cgacgactcc acgccgaaga tgtgtccgta tttggcaagc tgcttcggcg
     1141 tatcacgcgt aggatccaga ctgatggaca ggagtacggc ccggtcctgg agaccctcgc
     1201 gtaacagttg tacctgaagt tctttcagcg ccatgccggc caagggacag ccaagtcggt
     1261 cggagcaggc ggtatagatg aagctgacga ccgccacctt gccgtgcgta agagaaccgg
     1321 tgttgacgcg gcggcccgtg gcatcgatca gaacgaaggc cgggacatgc ttgatcacag
     1381 gaaggtcgta ggttccggcg gcaggcgggg tgaacttggg cgagaacacc ccctgaatat
     1441 aggtctcctc ccattcaggg atctcgtggg cctcgacgga cgggagaaga cacgccgcca
     1501 ggaacgcgcc gatcaccaca aaccaatacc ctatgtgccg tcggcgcgcc cacgggcatg
     1561 aaagtccgag gcgaggactc ccgcgccgcg agttgtcatt gcgagcgacc gaagggagcg
     1621 cggcaatctc accgtagctg cactgagaga ttgcttcggt cgctgtgctc cctcgcaatg
     1681 accccgaggg actttttggg cagcgcatga ctactcaacc ttgatgcgta gcgccttgct
     1741 cccaaagttc atcaggtgag cccggcccac cgcgttgaag tccgtggtga acttgtgcac
     1801 gagcttcccg ttctcccacg cataggcctt cagccagtag tcccccggct tatcccattt
     1861 cgagaggagc gagttggtga cgtagaggcg cgtaccgtcc cacgattcgg acaccatatt
     1921 ggccatctca cccaccttta cctgttcgat caacttgcct tcgaagggat tggagatgtc
     1981 gtatactcgt agcatcccgt ccatgaatga tgtgacgtag agcttgctgt cgtccggcgc
     2041 gatgctgata tctaccggaa gagtatcgcc gaggtcggcg atggacttgg cagcccaggt
     2101 gccgtccgct tgctggtaga tgaggacgag ttggtgggca agcgccgtcg ccgtaaaggc
     2161 gtaataatga ttcggcatga gcgcccagcg cagttccagc ggtgcgccag ggacctgcag
     2221 gacctgcagc ggcttgcggg catggaagtc ccacacgacg accgtaccgc cgaacgtctt
     2281 catcgcctcc gcatccttaa ccagctcgcc gaggggccgc atgtagttct tcttgccggt
     2341 gaaggaggag gtcagcatac gatttaggtt cacattgacc cgcacatcat agccataagg
     2401 cgcctccttc ggcatccaga tcgttcggat aaaacgcccc tcgttggagt actccgccag
     2461 tcccgtccgg cctgattggt ccttggcatt cgagagcgcc gagatcaaca tccgtcccgg
     2521 cagggcatag aaggtgtggg gaccgaccag gccacccgtg tctttcacaa agctcgtgat
     2581 cgttttgacc agcttgggcc tggctggatc tgaggcaaca tcgaagatga agatagagct
     2641 gtcgtccaga cctccggccc acagaaaccg ccggtcgtcg gtaaagccgg catggtgggc
     2701 ctcatgccgc ccgggcaccg gagcccgacg gatgatctgc ccgtaggcct tggacttggg
     2761 gttcacgtca acggtgacga ggctgtcgtt accgtcgccc accccctcga tccccagcgt
     2821 ccagacatag acatagtcct cctgcccggt cacctttggg aggaacggtg actggcaggt
     2881 ctcatcggcg acagccggcc cagccatcaa ggtgatcgcc atcagccaga gccatcctac
     2941 cgggcgatga tatggtctca tgcttgttcc ctccctgcat tatcgtttcc cacatgctca
     3001 gcaacacaaa cgcgttgccg agcctgtccg cgcccatctc ctggccttcg actctttgat
     3061 atcgtgatcc gcgaggttat ttcggtttct cccttgcttc gccgtccagc ccttccagtg
     3121 tcaaggcgac caaggtattg cccggcgctg ttgtgacccg cgtcagctcc agcagggccg
     3181 cgacgccgct ggcattgtcg ttggcgcctg gggtcggaag acgttgcgct caccgatctc
     3241 gccggcgagg cgctccacgt gcgttcgcag acgtgaagct gagatctgct ggacgatgct
     3301 cacggacccc aagcctggtt tcggcgtgcg cgccatcctc tcatccagtg aagccagatc
     3361 acgctgggtt cgatgctgag acatgcgggc cccttgttga gacattgcgg aatcggatgt
     3421 cacaatggct cctaaaaggc atgctaccag aaagcgcgtc gttagaccag aaacccgatc
     3481 cggattcaat ggccacatat catcgcttcg tgcggctctt ggtctcacaa gagggtcagt
     3541 tcaaccctca agctagagcc gatcctcacc gggacccacg tcggcgcgtc acaaaaacac
     3601 ttgacagatc cgtccgatca gaaggaatat cggcctgctc gtgatgatcc ttccccttgt
     3661 acacctattg tacacggaac acgagcgggt gcgcgtcttc catccatccg aaatcatcaa
     3721 cagggggcaa agagatgagg tcagttgtgg cggtgggtct ggcagtgctc gtcatggcgt
     3781 ccagcacgcc gaactgggcg gaggcggaag cgcaagaaag cacgctgtcg caagtcggga
     3841 caggggtcgg aagcgcgata gggaccttcg tctatttccc gttcaaggca gctttctgca
     3901 ttctaggggg agtggcaagc ggctttaccg tagtctttgc tggacctgag aacgcaggca
     3961 aggtcgccag aaccgcctgc ggagggagct gggtcattac gccagacgtt gtcaagggga
     4021 aggagacggt gaagtttgcg ggagatcctg cgccctcccg gcagcgtcca tccgccaagt
     4081 aagggagagt ggtcaccggg gatctgccct ggtatggaca gacccccgtc ccatgacgct
     4141 attgcatcgc ctccgcgacc cgtccgaccg gttgggcgga aggtgtgagg gttttgtctc
     4201 ctgcctcctt ccactgagcg ggacagacct catgaggatg atcggcgaga tagcgatttg
     4261 ccttcacctt ccgaaggagc tcgtcgatat tcctgcctac gttatagaag tggatctcgg
     4321 cgctcactaa tttcccttcc ggactgatga tgaaggtccc cctgagcgga aggccggtct
     4381 gctcatcata gaccccaaac agccgagcga tagtccccgt gggatccgcc cccatcggat
     4441 atctggcgcc ttgcaggagt ttttcctctc gctgtcaggc caagtgaacg aacttggtgg
     4501 cggtactcac cgtaatgacc tcggtctgaa gcgcccgcaa ctgttcgtac cgctctgcca
     4561 gagcggcatg ttccgttgct cagacgaagg taaaatcggc cgggtagaag aagagcaccg
     4621 tccacttctc cttctgcttg agcgtttcaa gactgaccgt cccgaacccc ccgttggccg
     4681 gctcaaatgt ctccaactga aactgggaaa ctggggcacc tcgtccccta cctcctcact
     4741 acagcgctga tcgtctgtca tctgcgtgcc tcctgttgtc gtgctgactc caaaggatgg
     4801 accatgcctc attcatctca cctggaggcc gatcgctcgt ctcccagacg ctacatcata
     4861 gctgcgtcct gaaatactga cagtaggtgt gcccgctatt ggtcggtaga gccactttca
     4921 atacatctgg atgcatgagt gtctcacacg tgactcaaag ccttgagaac gaggtgaagg
     4981 gggcatcaga tattggctca ggggagtcgg gcgtctcgct aacccgctga gcgatcgccc
     5041 gcatgctgcc cgcgcatgta agagacccga cgtgttctct cccagttcct gaaaacaggg
     5101 gaacaattta cactagtcgt acgtagtcaa atgcgaagca ctgctgtaac tacctaagaa
     5161 atcagagggg gctggttggc acggggattg ccttgagctg aggtgaaccc ccttgatgat
     5221 cgaatgagga gggcagcaac acgaccgact gcccactgag gcacgaagaa aggagcgagt
     5281 tggaatgggg atgggggtgc gggtggcgat gattcttggg ctaaggcttg ccaagccgat
     5341 accagttggc cccggtcagg aaataagggg ataagcggtc gctgtgcgat caacaccgcc
     5401 aacccctttg atccggaacg tcgaggggga aaaaggagac agcgatgtca ggccagaggt
     5461 ggaatccgtg gaagctcact acaatcggga tagcggtggt ctttgccacg gcactgatca
     5521 ctggactggt ggtagcaaac tgggttggca atcagcagac gaagccggtt gccgaggtcc
     5581 ctcgagggcg tgtctcgcgc gccgtttcgc cgcggatgac gccgtccccc gcagcgaggc
     5641 cgtcggcttc ggccgtcgcg gcttgtaatc agtatgcgag ctccatggcc ggcgacagga
     5701 ctaccgaagt cgtgaaggac gccttggtcg gaggggcgct cggcgcaggc gtgggcgcgg
     5761 ctggcggcgc gatcgccgga ggcggaaaag gcgccggcaa gggagccggc atcggaggcc
     5821 tggtgggagc gactgcaggc actctgtacg gcctcagcga ggccaggcag acggatgccc
     5881 gctacgttgc tgcctaccga ggctgcatga ggagccgggg ctacagcggc tagttcgtgc
     5941 tcgcttccag cccaggcagc caaggcagtg aagaacgaca aactgccaag agcgggtgtc
     6001 ctgatagcgg gcccgtgatc atagtattct tgacccccat ctgtcgcaac tgcggcatcc
     6061 ccatgtcctt cctgttcggc ctcactgcta gatgtagttg aacgcccatc atctttacga
     6121 ttccttaaca gcaaccccgc gaattttaac ggccttttga tttgacccga caagacctcg
     6181 atagtacgtt gactatggag ggtcgtgctg cgcaacctgt cgcctcagca ggcgagctca
     6241 cgtcaggccc accgactctc gaggacgttg accacgaact gagagttagc catgcctatg
     6301 atcgacgaag agaccgagct caacgtattt gaacgagttg cagagtacca gcccgctccc
     6361 tcgtggcgta cctggctctt tggccgcccg ctacagagta aggagccgag gagccgcaat
     6421 gctgggatga cgctgatctg gatgtcggcc gtcctgggca cgctgttcct gggcatcacc
     6481 ttcctggcgg tcaaggtcgg cgccatcccc gctgagcaag agacggtgat ctcgcaactg
     6541 gtccgtacag tattccaggg gcaggggctg ctctatctgg ccaccatcgc gggcacgacc
     6601 ctcatcctgg ttatggccgc caacaccgcc tttgcggact tcccacgact gagcgccctg
     6661 caagccggcg acggcttttt gcctcgccaa cttacctacc gaggcagccg gctcgttttc
     6721 tcgcgtggca ttgtcgccct tgcccttatt gcctccctcc tgatt
//
LOCUS       DATMXI010000327         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_71878, whole genome shotgun sequence.
ACCESSION   DATMXI010000327 DATMXI010000000
VERSION     DATMXI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71878"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VN444_06375"
     CDS             <1..945
                     /locus_tag="VN444_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-ammonia-ligase adenylyltransferase"
                     /protein_id="HWR21467.1"
                     /translation="AYRLAREDLRLQYGLPSSAGFVVLGLGKCGAEEMGYGSDLDLAF
                     AYAQEGTTTGGSKYISHAEYFGRLADKVCKNLTTITKEGTVYRVDIRLRPGGSVGRVA
                     QSFAAFETHFSRTAELWERQAYLRARPIAGDLDIAEPFMTLLSELICRPTSVESLAAN
                     ITAMRHRMEVELTKEKTGERHVKLGSGGIVDIEFIAQFLQLAYGSTLSALRVGNTLKA
                     VESARSAGLIAGSDAVDLSTSYRFLRTVQNRLRIVADLETSVLPNDPDRLDRLARRLG
                     YEPADGSRSGERFLADYQRHTERVRGIYETTFHPPLRT"
     gene            1165..1605
                     /gene="rnpB"
                     /locus_tag="VN444_06380"
     ncRNA           1165..1605
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN444_06380"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2050..2181
                     /locus_tag="VN444_06385"
     CDS             2050..2181
                     /locus_tag="VN444_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21468.1"
                     /translation="MAKRTIRARDLGIDLESGTEGERFKWFLACLLFDKPIQREVAR"
     gene            2212..2499
                     /locus_tag="VN444_06390"
     CDS             2212..2499
                     /locus_tag="VN444_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21469.1"
                     /translation="MTPQALLDAGWDRIHYVRVDFSTATKLLDLTQSLTTRYGSLTNL
                     LRQSGSRKDLALRLQEFKGIGPVTTRIFLRNIKSHSGEPFRPGLAERSKTQ"
     gene            2581..2982
                     /locus_tag="VN444_06395"
     CDS             2581..2982
                     /locus_tag="VN444_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21470.1"
                     /translation="MEKDSDQEYVQLCLTIRHYSALRFAMRTLFLALFIGLAVIGFGI
                     VPQQSFWVMATAKTLGFLTTCFFWACEKNAARYMSHMQERAAELERLLGYRLWSGMPQ
                     SVYWFVGPPFVTPLVYGVIALFWLYAVAFVR"
BASE COUNT          631 a          858 c          958 g          714 t
ORIGIN      
        1 gcatatcgac tggctcgtga ggacctgagg ctgcagtatg gtctaccgtc ctcggcaggg
       61 ttcgtcgtct tgggccttgg caaatgcggt gccgaggaga tgggttatgg gtccgacctt
      121 gatcttgcct ttgcttatgc gcaggaagga acgaccacag gcgggtccaa gtacatcagc
      181 catgccgaat attttgggcg cctggccgac aaggtttgta agaacctcac gactatcacc
      241 aaggagggaa cggtctaccg ggtcgacatc cggctgcgcc caggtggatc ggtaggcagg
      301 gtagcacagt ccttcgcggc atttgagact cacttttcca gaacagccga gctgtgggag
      361 cgccaggcgt acctcagggc gcgtccgatc gcgggtgacc tcgacatcgc ggagccgttt
      421 atgactttgt tatccgagct tatctgtcga ccaacctcag tggagagcct ggcagcgaac
      481 atcacggcga tgcggcaccg gatggaggtg gagctgacca aggagaaaac cggtgaacgt
      541 catgtgaagt tggggagcgg tgggatcgtt gacatcgagt tcatcgccca gttccttcag
      601 ctcgcgtatg gatccacgct ctccgccttg cgggtcggca atacgctgaa ggccgtcgaa
      661 tcagcccgca gtgccggtct gatcgccggc tcggatgctg ttgacctcag tacgtcatat
      721 cggttcctac gcaccgtgca gaatcgtctt cggatcgtag ccgatcttga gacctccgtg
      781 ttgcctaacg atccagaccg gctcgatcgc ctggcacgac ggcttgggta tgaaccggct
      841 gacggctcac gttcagggga gcgatttctg gctgattacc agcgccatac ggagcgggta
      901 cgggggattt acgagacgac cttccatccc cctctaagaa cttgaggggg caggcgctat
      961 aatccttgtc cccgcatgtt ctcagcgggg agcggtgaaa ccgggagccg acgcgagagc
     1021 ctatgatgcg taggagggat ttcagtctac gcatccttcg accagctcag ggtgaacggt
     1081 gaacgcaaag aagcgttaga cgcacacccc tagcaaggat cgctgttgca aatcgtgcca
     1141 gtgtgcgtta cgttagtagt ggtcgaagtc ggtcggatga ccgccggttc gcctttggcg
     1201 gacgggagga aagtccggac tccgcagggt agggtgctgg gtaacgccca gggagggtga
     1261 cctcacggac agtgccacag aaaagatacc gcccacgctg catcatcggc cgccaggctt
     1321 cgtcacacgc gtagccgaac ggtcccgtgg gtaagggtga aatggtgcgg taagagcgca
     1381 ccagcggcat ggtgacatgt cggcttggca aaccccaccc ggagcaaggc caaataggtc
     1441 ccgccccatc atgcagagag cggcccgctc tcgtgttcct ttcggggcag gcgggacggg
     1501 taggctgctt gagcccgacg gtaacgccgg gcctagagga atggtcatcc ggtctggccg
     1561 caaggtccgg ccgtacagga tccggcttat agaccgactt cggccatatc aattgagtca
     1621 agtaagtgtc acggttttga agagcggtaa gcgggcgtaa cataatcgtg gtctgaaatc
     1681 tcacacctcg agaggcgcgt taatgttcgg acttgacgga ttcgatattg tttgggtggt
     1741 tatgatcggc gccttagtga tcggatatgg cctggtgtgg attgtctttc gacgtatttc
     1801 tcggaagtaa gagttattct ttatggatac cgattgaggc tgccggtgcc ccaggatggt
     1861 ataaggggcg gcccctccag gcttctacgc tgcagccgtc tggtgcagcc gcgtgctagg
     1921 cttcggatct cgccggtgga tgagcgtggc tctctccatg tgccgctgca acgtcccgca
     1981 tgtagtgcta gatactattg catagaacct cattgccaaa tgccttcgcc gcggagacga
     2041 ccatcgtcga tggccaaacg taccatccgc gctcgggacc tcgggatcga cctcgaatcg
     2101 gggacagaag gcgagcgctt caaatggttt ttggcctgcc tcttgttcga taaaccgatt
     2161 caacgggagg tggccaggtg agcctatctg gaattcgcta aagaggggtt ggtgaccccg
     2221 caggcgcttc tcgatgcggg gtgggaccgc atccactatg ttcgcgtcga tttttcgacg
     2281 gccaccaaac tccttgacct tactcaatcc ctcacaaccc gctatggttc cctcacgaac
     2341 ctgctaagac agtccggcag caggaaggat cttgccctgc gtctccagga gttcaagggc
     2401 ataggtcctg ttacgacccg catcttcctc cgcaacatca agtcccattc gggggaaccc
     2461 ttcagacccg gacttgcaga acgcagcaaa acgcagtagt gtgacaaggg ggattgcacg
     2521 cttgggtgcc tcagggtaag gaaggcgggc ccatcccgcc aggaaggatg gagagacccg
     2581 atggaaaagg attctgatca ggaatatgtt cagttgtgtt tgaccatcag acactacagt
     2641 gcgcttcgct ttgccatgcg gaccctattc ttggcactct tcatcggtct agcggtcatt
     2701 ggtttcggga tcgttcccca acaatctttt tgggtgatgg ccacggcaaa gacactcggc
     2761 ttcttgacaa cgtgtttctt ctgggcgtgt gaaaagaacg cggcgcgcta tatgagccac
     2821 atgcaggaaa gagcggctga gctggagaga ctgcttggct atcgcttatg gtcagggatg
     2881 ccccagtcgg tgtactggtt tgtaggtccc cccttcgtca ccccacttgt ctatggagtg
     2941 attgcgctat tctggctata cgcggtagcc tttgttcgtt gatgtagagc ttttcttcaa
     3001 cttcttccca tttgctctgc catttgcccc tcgtcctttt gcctccggtc cttttgttga
     3061 agagtggccg tggacgctca ttgctgtcaa cgtcggtaga tcatcccctg tagtctcagg
     3121 cgtatcagcc ggatcgccgt acatcgggtg aagcttcctg a
//
LOCUS       DATMXI010000328         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_141571, whole genome shotgun sequence.
ACCESSION   DATMXI010000328 DATMXI010000000
VERSION     DATMXI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141571"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VN444_06400"
     CDS             <1..202
                     /locus_tag="VN444_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HWR21471.1"
                     /translation="QIVDIFDALRTARPYKPALSLKTACDALRDEVARGWRDPGVVEP
                     FIELVELGGPSEAPEPAGEAGV"
     gene            241..471
                     /locus_tag="VN444_06405"
     CDS             241..471
                     /locus_tag="VN444_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21472.1"
                     /translation="MAFAEILIPFVAVTGIAMVASRTIRQSQSTIQEEMLDFDGIRSI
                     GKVNGPIVELSGHGAHLGSGRVAAFNQNHWHL"
     gene            886..2391
                     /gene="lysS"
                     /locus_tag="VN444_06410"
     CDS             886..2391
                     /gene="lysS"
                     /locus_tag="VN444_06410"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWR21473.1"
                     /translation="MSEERSLIGERMRKLAELEAAGIDPYPARFGVTHLAADLHREYA
                     GLSEEGDAGAPQVSIAGRLMSVRGHGKATFAHLQDGSGRIQIYIVRDTVGLEAYDLCK
                     KLDVGDYLGVEGQLFRTRTGELTVRAMTVQLLSKSLRPLPEKWHGLTDVETRFRQRYL
                     DLIVNRQVADAFRTRSRVITEIRRFLESRRFLEVETPMMQAMAGGAMARPFVTHHNAL
                     DLTLYLRIAPELYLKRLVVGGFDRVFEINRSFRNEGISTKHNPEFTMLEFYQAYADYR
                     DLMATTEELLAHLAKEITGDQEVTYQGQRISFAAPWPKLTLEEALVKLAGLDAEALTT
                     EDGVSATARRHGVSVSPDWGRGKVLAELFDALVEPKLLQPTFIIDFPTELSPLAKATR
                     GEPTTVQRFELFVGGMEIANAYSELNDPREQRARFLDQLRQRDQGDLEAHGLDEDYLR
                     ALEYGMPPTAGEGIGIDRLAMLFTDSASIRDVILFPLLKPAQGEHDGTDAV"
     gene            2381..>2783
                     /locus_tag="VN444_06415"
     CDS             2381..>2783
                     /locus_tag="VN444_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR21474.1"
                     /translation="MPFELFVGLRYLKARRGQAFISLITLISIGGVALGVMALIVVLA
                     VMSGFERDLRGKILGTNAHLWIIRHGDRGIDEPAQTLVRVRDVPHVVAVSPFTYHQVM
                     LSTGRGAGGAVLRGIDLDSAQEVTTLTKSFTE"
BASE COUNT          543 a          764 c          874 g          602 t
ORIGIN      
        1 gcaaatcgta gatatctttg atgcgctgag gactgcgcga ccatacaaac ctgccctgtc
       61 gctaaaaacc gcttgcgacg ccttgcggga tgaggtggcg cgtggctggc gtgatccagg
      121 tgtggttgag ccattcatcg agctggtcga gctgggcggg ccgtcggagg ccccggagcc
      181 tgccggtgaa gcaggggtat gagacggatt cgggactgga gtactcgcag gtgccttttc
      241 ttggccttcg ccgagatttt gatccccttt gttgctgtta cgggtattgc catggtagcg
      301 tcccgcacca tccgccagag tcagtcgacc atccaggagg agatgctcga tttcgatgga
      361 atacggagta tcggcaaagt taacggacct atcgtagaac tcagcggaca tggtgctcat
      421 ttgggttcgg gacgggtggc cgctttcaat cagaatcact ggcatttgta gaccggaata
      481 cgcaacaaga cgcgggacaa ttttcggcta tccggattga cttgttgaaa ataagtagtg
      541 tattctctct gctcgtttcg gattgatctg tgaaattact ggaatgagcg ctccacaggc
      601 gttcaaagtg ccaggacttt gagcggcttg gcaccgtttc acttgtcgat caatcacatg
      661 cagaatcacc gcttggcacg agctcgtcgc tccactcctc gatttctccc ttggtgtttc
      721 ccgcctctct ccagccgccg gctctcttcc aaggagggga gattgctcac gctaacctga
      781 agatggcagc cgattctcag acagcaagaa gaaaatagga tctgaagtgc cttgacaccc
      841 gttacccccc cttcctataa tcggaccaat tcgacaggag gtcaagtgag cgaagagcgt
      901 tcgttaatag gcgagcggat gcggaagctg gcggagcttg aagcagccgg tattgacccg
      961 tatccagccc ggttcggtgt cactcatctc gcggccgatc tgcaccggga gtatgcgggt
     1021 ctctctgaag agggggacgc gggggctcct caggtgtcga tcgccgggcg cctgatgtcc
     1081 gtacggggtc atgggaaggc gaccttcgct cacctccagg acggttccgg gaggattcag
     1141 atctacatcg tacgggatac cgtcggtctc gaggcatacg acctctgcaa gaagctggat
     1201 gttggcgact acctgggggt ggaaggccag ctcttccgga cgcgcacagg agagctcacg
     1261 gtgcgagcca tgacggttca gctcctgtcg aagtcgctgc ggccactgcc ggaaaagtgg
     1321 catgggctga ccgacgtgga gacccgtttc cgtcagcgct atctggatct catcgtgaac
     1381 cgccaggtcg cggacgcctt caggacgcgt agccgcgtaa tcacggagat tcgccgcttt
     1441 cttgaatcga ggagattcct ggaagtcgag acccccatga tgcaggcgat ggctggcggc
     1501 gccatggcgc gcccatttgt cacacatcac aacgccctcg acctcacgct gtatctgagg
     1561 attgccccag agctctacct gaagcggctc gtggtgggag ggttcgatcg agtcttcgag
     1621 atcaaccgga gctttcggaa tgaggggatc tctaccaaac ataaccctga gttcacgatg
     1681 ctggagttct accaggcgta tgccgattac cgggatttga tggcgacgac ggaagagctg
     1741 cttgctcatc tggccaagga gatcacggga gaccaggagg tgacctatca gggacagcgg
     1801 atctccttcg ccgcaccatg gccgaagctt acgctggagg aggcccttgt caagctggct
     1861 gggcttgatg cagaggccct cacaacggaa gacggcgtga gcgcgacggc caggcgtcat
     1921 ggagtaagcg tctcgccgga ttggggcaga ggaaaggtgc tggcggagct gttcgacgcc
     1981 ctggtggagc cgaagctctt gcagcccacg ttcattatcg attttcccac cgaactctcc
     2041 cccttggcca aagcgacacg aggggagccg acgacggttc agcggtttga gctgttcgtt
     2101 ggcgggatgg agatcgccaa cgcctactct gagttgaacg atccgcgcga gcagcgcgcc
     2161 cgctttcttg accagttgcg gcagcgggat caaggcgatc tggaggccca tggcctggat
     2221 gaggattatc tgcgtgcatt ggagtacggg atgcctccga cggctggtga ggggatcggt
     2281 atcgaccggc tggctatgct cttcaccgac tccgcctcca ttcgtgacgt tattctcttt
     2341 cctcttctga agcccgccca aggcgaacat gacggtaccg atgccgtttg aactgttcgt
     2401 gggactccgg tatctgaagg cgaggcgagg gcaggcattc atctccctga ttacgctgat
     2461 ctctatcggc ggcgtcgccc ttggcgtcat ggcgctcatt gtcgtcctgg cggtgatgag
     2521 cgggtttgag cgtgaccttc ggggcaagat cctcgggacc aatgcccacc tctggatcat
     2581 acgccatggg gatcgaggga tagatgagcc ggctcagacg cttgtgcggg ttcgcgatgt
     2641 tccgcacgtg gtggccgttt ctcccttcac ctaccaccag gtgatgctga gcacgggtcg
     2701 aggggcagga ggggcggtcc ttcgtggtat cgatctcgac tccgcgcagg aggtcacgac
     2761 gctgacaaag agcttcactg agg
//
LOCUS       DATMXI010000329         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_30797, whole genome shotgun sequence.
ACCESSION   DATMXI010000329 DATMXI010000000
VERSION     DATMXI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30797"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VN444_06420"
     CDS             <1..708
                     /locus_tag="VN444_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21475.1"
                     /translation="VLTLGLSPTEITQAHVGLRPGEKMHEALWEDSEEMLASDHARIF
                     VLRQHAKPLSEVEALIGEMEDLAMSGNFTRLLRKIQEAVPSYSPPDKAPLSVVPEVSE
                     QYRVLVIDDNEQMCDVLAKALTLNGMFKVTIAHTAREGFDRIKAEVPHLILLDVKLPD
                     KSGLEICQILRSHPAFNQIPIIMMTGYTQEADVVTGLQAGADDYLCKPFRIEELLARV
                     EAMLRRGAGLPTAGSVV"
     gene            723..1709
                     /locus_tag="VN444_06425"
     CDS             723..1709
                     /locus_tag="VN444_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR21476.1"
                     /translation="MSETPRVSIGLPVYNGERFLREALDSLVGQTFGDFELIISDNGS
                     TDGTQEICRAYAAKDRRIRYYRQDQNHGGAWNHNRVFELSKGEYFKWASHDDVCDPAL
                     LSRCVEVLDADPSVVLCYSKVRIIDEHGRVENYAVELATDSPNPQERFRDLMLIPHRC
                     FQIYGLIRSRDLKMTPLFAACSAADRPLLARLGLAGRFHEVPEYLFVSRWHSRNSMRA
                     TRSAQVRIAWWDPAMAGKITFPFWRIFREYFLAIRDAQLTRQERMRCYLYLLRWPLVR
                     GQNRHWLTAHWVELGKDLVKALIWPAYIAYASRTSDGSSSRTEPAVQGRRYQ"
     gene            1706..>2224
                     /locus_tag="VN444_06430"
     CDS             1706..>2224
                     /locus_tag="VN444_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWR21477.1"
                     /translation="MKVAILAGGEGTRLVEETQVKPKPMVEIGGQPILWHIMMHYSHY
                     GFNQFVIALGYKGEVIKKYMVDYCSLNSDLMIRLKTGEVKRSNGSAPDWTIELADTGI
                     TTFTGGRIKRLAPYIGDETFMLTWGDGVSDVDLNELLAFHRSHGKLATLTAVRPPARF
                     GHLVFNGDQVTQF"
BASE COUNT          501 a          535 c          655 g          533 t
ORIGIN      
        1 gtactgactc ttggcctttc cccaaccgag attacccaag cgcacgtggg actacggcct
       61 ggggagaaga tgcacgaggc tctctgggaa gatagcgaag agatgctggc gtccgaccat
      121 gcgaggatct tcgtccttcg acagcacgcg aaaccgctgt ccgaagtcga ggcgcttatt
      181 ggcgagatgg aggatctcgc gatgtctggt aacttcactc ggcttctccg aaagattcag
      241 gaagctgttc cgagttattc cccacccgac aaggcgccac tctctgtggt cccggaggtg
      301 agtgaacaat accgcgtgct ggtgattgac gataatgagc agatgtgcga tgtgttggca
      361 aaagccttaa ccttgaatgg catgttcaaa gtgacgatag cccataccgc ccgcgaggga
      421 tttgaccgga tcaaggctga ggttccccat ctgatccttc tcgatgtcaa gctaccggat
      481 aagagcggcc tcgagatatg tcagatactc cgttcgcatc ctgcctttaa tcagattccg
      541 attatcatga tgacggggta tacgcaagag gccgacgtcg ttacaggact gcaggccggc
      601 gcagatgatt acctctgcaa gccattcaga atagaggagt tgttggcgag ggtcgaggcg
      661 atgttgaggc gtggggccgg cttgcccact gccggttctg tcgtataggt acggagggag
      721 gcatgagcga aacaccccgc gtcagtatcg gactcccggt ctacaatggc gaacggtttc
      781 tcagagaagc cctggactca ctggtgggtc aaacctttgg ggactttgag ctcatcatct
      841 cggataatgg ctcgaccgat ggcactcagg aaatatgccg cgcctacgca gccaaagacc
      901 gtcggatccg ttactatcgt caagaccaga atcacggtgg agcgtggaac cacaatcgag
      961 tgtttgagct gtcgaagggt gagtacttta agtgggcctc gcatgatgac gtgtgcgatc
     1021 ctgccctttt atctcggtgc gttgaggtcc ttgacgccga tccttcggtg gtattgtgtt
     1081 actccaaggt caggatcatt gatgagcatg ggagggttga gaactatgct gtggagctgg
     1141 caactgattc acccaatccg caggagcgtt ttcgtgacct catgctaatt cctcatcgct
     1201 gctttcagat atatgggctg atacgctcaa gggacctgaa gatgaccccg ctttttgcag
     1261 cgtgctcggc agcagatagg ccgttgctgg ctaggctggg tctggccggt cgcttccacg
     1321 aggttccaga atatctcttc gtgtccagat ggcattcaag aaattcgatg agggctacac
     1381 ggagcgctca ggttcgcatt gcgtggtggg atccggcaat ggcagggaag atcacttttc
     1441 cattttggag gatatttcga gagtactttc ttgcgatacg ggacgctcag cttactcgac
     1501 aggagcgtat gcgttgctac ctttatttgc tacgttggcc gttggttcgt gggcagaacc
     1561 gacactggct gacggcacac tgggtggaac tcgggaagga tctggtaaag gctttgatat
     1621 ggcctgcgta tatcgcttac gcctcgagaa catcagacgg tagctcgagt agaacggaac
     1681 ctgccgtgca ggggaggaga taccaatgaa agttgccatt cttgccgggg gggaaggtac
     1741 gcgcctcgtt gaagaaaccc aagtgaagcc aaaaccaatg gtagaaatcg gtgggcaacc
     1801 gattctctgg cacatcatga tgcactattc ccattatggc ttcaatcagt tcgtaatcgc
     1861 gctcgggtat aagggtgagg tcattaagaa atatatggtg gactactgtt cgttgaatag
     1921 tgatctgatg attaggctga agaccggaga ggtgaaacga agcaacggca gtgcgcctga
     1981 ttggactatt gagttggcag acaccggaat taccacgttt accggagggc gcattaagcg
     2041 ccttgcgccc tatataggag atgagacatt tatgctcaca tggggggatg gcgtgtccga
     2101 tgtggatttg aacgagttgt tagccttcca tcgctctcac ggcaagttgg ctaccttgac
     2161 tgccgttcga cctccagcgc ggtttggcca tcttgttttc aatggcgacc aagttacgca
     2221 attc
//
LOCUS       DATMXI010000330         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_116908, whole genome shotgun sequence.
ACCESSION   DATMXI010000330 DATMXI010000000
VERSION     DATMXI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116908"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..977)
                     /locus_tag="VN444_06435"
     CDS             complement(165..977)
                     /locus_tag="VN444_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWR21478.1"
                     /translation="MGFIGSLFEGGGKSGKKLIEASKSGDTKTVKSLLAASADTNARD
                     QHGWTALMHASWYGYAGVAELLLNHGAQVNATDQDGWTALMRASWHGYAGVAELLLNH
                     GAEVNVGEKHGWTALMRASGHGRTGVAELLLKQGAEVNARDQHGWTALMHASGNGRTG
                     VAELLLNHGAQVNATDQDGWTALMRASWHGHTEVSELLLNHGAQVNARNNTGGTALMR
                     ASEKRHSETVGMLLNHGAEVNVRDNTGGTALLRASGNRHSLVAELLKRHGAS"
     gene            complement(1170..2378)
                     /locus_tag="VN444_06440"
     CDS             complement(1170..2378)
                     /locus_tag="VN444_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR21479.1"
                     /translation="MDRPGERQPIILSGSTHRGRESSIDATISSFNFFVASLANFFFF
                     ASLNAFSLLPLYIKTLGGTESQIGWIMGSYSLTAILWQPLAGAFVDRFGRKRFLLLGS
                     ALGLLASVGFAFSTQMDARFVVFRILQGIGYSTFYIANLTLVAEIVPSNRRGEAVGLF
                     GISGLITIALSPALGEIVIHRAGYSVFFLAAAAAAAGCLLTSMAFRNLSATPLTTVSS
                     GPSFLIPSARILPPILLTLVFGLVSGAVFVFLPTYAMQAGLSRIGGFYIAYSVAAVGI
                     RLTCGRLSDRWGRQRVILPAMVLMGSGTLGLVWLVSPLGLLLVGTLTGMAHGLLFPAL
                     SAYAIDLADPEERGRSIGAFTTAMLLGNALASFIFGMVAEQFGYPMIYLLTSGIVTMT
                     FFVFLRFHRT"
     gene            2669..2899
                     /locus_tag="VN444_06445"
     CDS             2669..2899
                     /locus_tag="VN444_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR21480.1"
                     /translation="MTTTAYVLIEGASDQTATIVKALQKIKGVKSAHAVTGPYDVIAC
                     VEAIDVGTVGTVILSKIRTLKGVTRTVTCVAV"
     gene            complement(3275..>3779)
                     /locus_tag="VN444_06450"
     CDS             complement(3275..>3779)
                     /locus_tag="VN444_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWR21481.1"
                     /translation="AMNGKTIEVINTDAEGRLILADALCYACDRGLTHLVDVATLTGA
                     VVIALGAVRTGAFTNDAELLRQVREASEAAGEKIWELPMDDEYAEQIKSDYADMKNVG
                     GRKAGPITGAKLLANFIGKSPWVHLDIAGTAQTEKDNGYQVKGATGAMVRTLICLAMS
                     FSGSRQG"
BASE COUNT          878 a         1137 c         1005 g          759 t
ORIGIN      
        1 cagtgcgtcc atctcgttca gtaagcaacc gcaaccgatc cttgccagga caccggattc
       61 gaggcgtccg ccccacaccc tgagatcctt tagatggggc ccatgagagt caagcgagca
      121 gcccgattga gaaagttcaa tgcgtgaaac ccggcgtctt tttcttagga cgccccgtgc
      181 ctcttcagca gttcagcaac cagagaatgc ctattgccag acgcacgcag caaggccgtc
      241 ccgccggtat tatccctcac gttcacctcg gcgccgtgat tgagcaacat ccctacagtc
      301 tcggaatgcc ttttctcaga cgcccgcatc agggccgtcc caccagtatt attcctcgcg
      361 ttcacctggg cgccgtgatt gagcaacagc tcagagacct cagtatgacc atgccaagac
      421 gctcgcatca gggccgtcca gccatcttga tccgtcgcgt tcacctgggc gccgtgattg
      481 agcaacagct ctgcaacgcc ggtacggcca ttcccggacg catgcatcag ggccgtccag
      541 ccatgttgat ccctcgcgtt tacctcagcg ccttgcttga gcaacagctc tgcaaccccg
      601 gttcgcccat gcccagacgc tcgcatcagg gccgtccagc catgtttttc gcccacgttc
      661 acctcggcgc cgtgattgag caacagctct gcaacgccgg catacccatg ccaagacgct
      721 cgcatcaggg ccgtccagcc atcttgatcc gtcgcgttca cctgggcgcc gtgattgagc
      781 aacagctctg caacgccggc atacccatac caagacgcat gcatcagggc cgtccagcca
      841 tgttgatccc tcgcgttcgt atcagcgctt gcagctaaca ggctcttgac tgttttggtg
      901 tctccagatt tagacgcctc gatgagcttc tttccagact tcccaccacc ctcaaataat
      961 gagccaatga aacccatttc acctcgcttc aggcctcaca ccccaacccg tccatcgggc
     1021 cgctgaggag tttcgttgcg atacctatct ctatcgtaga catcgaatat aaccacgtgc
     1081 gcctgccgct tccgaagcta tgggccacgg caatgttcgg gttattttca gataacggta
     1141 gagagctata gagcgtatat gtctttatac tacgtcctgt gaaaacgaag aaatacaaaa
     1201 aacgtcatcg taacaattcc cgaagtcaag aggtagatca tagggtagcc gaactgctct
     1261 gcgaccattc caaatatgaa cgacgcaagg gcgttgccca acaacattgc agtggtaaac
     1321 gcgccaatgg agcgtccgcg ttcctccggg tctgcgaggt cgattgcgta ggcgctcagg
     1381 gccgggaaaa gtaagccgtg agccattcct gttagcgtcc caaccagcag cagaccaagt
     1441 ggagagacga gccagacaag gcctagcgtg ccggatccca taagcaccat ggcgggaagg
     1501 atcacgcgct gacgccccca tcgatcagac agtctgccac aggtcagccg gatgccgaca
     1561 gcagccacgc tgtacgcgat gtagaaccca ccgatacgcg acaacccagc ctgcatcgca
     1621 taggtcggta ggaatacgaa caccgccccg gagaccaaac cgaacaccag cgtaaggaga
     1681 attggcggaa ggatacgcgc tgatgggatc agaaaagagg gaccggaaga gacagttgtg
     1741 aggggcgtcg ctgagagatt acggaacgcc atgctggtca gaagacagcc cgccgcggcc
     1801 gcagcggcgg ctaagaagaa aaccgagtac cccgcacgat ggatcacgat ctcaccgagg
     1861 gcgggagaga gagcgatcgt aatgagtccg gagatgccga aaagtcccac cgcctccccg
     1921 cgccggttcg atggaacgat ttcagccacc agggtgagat tggcgatata gaaggtggag
     1981 tatccgatac cttgcaggat gcgaaagaca acaaatcgcg catccatttg cgtcgagaac
     2041 gcaaagccga ccgaggccag caaccctaac gccgatccca gcaggaggaa tcgcttgcgt
     2101 ccgaagcggt caacgaaggc gccggccagc ggctgccaca gaatagccgt caggctgtag
     2161 ctccccatga tccaaccgat ctgcgattcg gtccccccga gggtcttgat gtaaagagga
     2221 agtaagctga aggcattgag actcgcgaaa aagaaaaaat tggcaagcga ggcaacaaag
     2281 aaattaaagc tggaaatcgt tgcgtcgata gagctctctc gcccccgatg ggttgagcct
     2341 gacagaatga tgggttgtct ctcgcctggt cgatccatgt gtacaagatc agcgcaggtg
     2401 cgagacgcga taagggtgtc gaaacacaac gacacgccct attgtgccct cacgcgcctt
     2461 ggcaggtcgg cccatctttc tgcttgtgta ccgccggcat ctatcgtata catacatgga
     2521 gtcatgattg tccgcccgcc agattgagcc actgaccagg gcataagaat agccagaggt
     2581 tcctgggccg acgttgtcgg cggaaagagt tggtgctttc atcataaccc caccttcacg
     2641 ctcggctatc caaaaggagg gatgcgctat gacaaccact gcgtatgtcc tgatcgaagg
     2701 cgcgtcggat cagacggcca ctatcgtgaa agcgctacag aaaataaaag gggtcaaatc
     2761 ggctcacgcc gtgaccgggc cctacgatgt gatcgcgtgc gttgaagcaa ttgatgtcgg
     2821 tacggttggg acggttatcc tctcgaaaat ccgcaccttg aagggtgtca caagaaccgt
     2881 gacctgcgtc gctgtctaag gcaaacacac atactgaagt gtcattgcga gcgactgaaa
     2941 ggagcgcggc aatctcgatt ctctcgcatg gatcacaata agattgcttt gtcactttgc
     3001 tcctcgcaat gaccatgcag gaatattgag tgcgtgtctc cgtaactacc caggtgttta
     3061 ggctgtaggc tgaaggctgt tagccgttgc gcatggaccg aatcaaaggg tccagaacct
     3121 tttcggtctc catcgcgttc gcgtcgcatt cagaaaacgc ctggctcatt cgtgcgcgtt
     3181 cctacagtct aaaagcctac agtctatcta cctgagtagc tacgtgtctc ctggataagc
     3241 cgcggcgaat cgtttccccc aatcttgcaa gtgctcaccc ctgcctggac ccggagaagc
     3301 tcatagccaa acagatcagg gttcggacca tggccccggt tgcccccttc acctgatacc
     3361 cgttgtcctt ttcggtttgc gcggtcccgg cgatgtccag gtgaacccat ggcgatttcc
     3421 cgataaagtt cgccagaagc ttcgcgccgg taatcggccc ggcctttcgc ccgccgacat
     3481 tcttcatgtc cgcataatcg ctcttgatct gttcggcgta ctcgtcatcc atgggcagct
     3541 cccagatctt ctcgccggcc gcttcgctcg cctctctcac ctgccgcagc agttcggcgt
     3601 cattggtaaa ggcaccagtc cgtactgccc caagggcgat gacaaccgcg ccggtgagtg
     3661 tcgccacgtc aacgaggtgg gtaaggcccc tgtcgcaggc gtaacagaga gcatccgcaa
     3721 ggatgagccg accttcagcg tcggtattga tcacctcaat cgtcttaccg ttcatggcc
//
LOCUS       DATMXI010000331         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_154384, whole genome shotgun sequence.
ACCESSION   DATMXI010000331 DATMXI010000000
VERSION     DATMXI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154384"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN444_06455"
     CDS             <1..339
                     /locus_tag="VN444_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWR21482.1"
                     /translation="ILNHRKAGYTGNVMVAWPVPEEKVEAVGHQMAQFRAVSHCYQRP
                     AYPEWPFTLFTMVHQKSREACEETVAAISRETGISDYAMLWTTREFKKVRLKYFTEDF
                     AAWDEKAIAR"
     gene            complement(584..925)
                     /gene="yajC"
                     /locus_tag="VN444_06460"
     CDS             complement(584..925)
                     /gene="yajC"
                     /locus_tag="VN444_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWR21483.1"
                     /translation="MGIAYAQGATNGSPPSGMLGALITPVAIFIIFYFLMIRPQQKKS
                     REQKDMLENLKTGDQIVTTGGLYGTIVKFGENNRVKVKLAENVTVEIARGAITEKAVE
                     TTEGTAKGKSD"
     gene            complement(1136..2266)
                     /gene="tgt"
                     /locus_tag="VN444_06465"
     CDS             complement(1136..2266)
                     /gene="tgt"
                     /locus_tag="VN444_06465"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWR21484.1"
                     /translation="MVTFELLKIDAATGARRGRLRTPHGAVETPAFMPCGTGGAVKAL
                     TPHELETEGVQMALCNTYHLYLRPGHRLIEELGGLHRFMAWSGSILTDSGGFQVYSMA
                     PLRQISEDGVSFRSHLDGSLHFLSPELAIEVQKSLGADIIMPLDECAPYPSTTDYLQR
                     SLGLTLKWAERSRVAHPNGQPALFGILQGGTDRALREQAATDLQKIGFDGYALGGLAV
                     GEPKAVMYETVAHTTPLMPVDRPRYLMGVGTPEDLVESVMRGVDMFDCVMPTRHGRTG
                     SLFTSQGRINIKGAAHASDERPLDPACDCYTCRHFSRAYLRHLFMAGEILGLRLNTLH
                     NLHFYLTLMRQIRQAIEDGSLAAFRTKFLEETGTLNRDETVA"
     gene            complement(2324..3442)
                     /gene="queA"
                     /locus_tag="VN444_06470"
     CDS             complement(2324..3442)
                     /gene="queA"
                     /locus_tag="VN444_06470"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWR21485.1"
                     /translation="MSLALRTADFDYILPPDLIAQAPAPERDRSRLLVLDRNTGVLQH
                     RIFRDLSEYLLPGDLLVINEAKVIPARLFGRSERRHLIEVLLLREVETVPGLRHAGTG
                     CWEALVKPSRQVRAGDRLTLADETITAEVMEKRGEGRHLLKLVYDGTLSDLLWRFGQM
                     PVPPYIKRQGAKSPHPPFTKGGHGGIWGVELDRERYQTVYAKHEGAIAAPTAGLHFTP
                     ELIETLTRQGVAVAPITLYVGPGTFRPVRVEEISAHQMEPERYIVPEQTALAVKAAKR
                     EGRRVIAVGTTTVRALEHAAVDGDVRSGAGVTDLFIYPGYRYRCIDALITNFHLPRST
                     LFMLVSAFAGRQAVVAAYREAIALRYRFYSYGDAMLIV"
     gene            complement(3477..3701)
                     /locus_tag="VN444_06475"
     CDS             complement(3477..3701)
                     /locus_tag="VN444_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWR21486.1"
                     /translation="MQGLESLAKLLIVFGLILAVVGGLILFVGKVPFIGRLPGDIYIQ
                     RKNFSFYFPLTTSILLSILLTILFSLFRRR"
     gene            complement(3753..4808)
                     /gene="ruvB"
                     /locus_tag="VN444_06480"
     CDS             complement(3753..4808)
                     /gene="ruvB"
                     /locus_tag="VN444_06480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWR21487.1"
                     /translation="MTSEKQKKESGPERVANRQLQDEDQDLELSLRPKAWDDYIGQEK
                     VKANLQVYVQGAKARREPLDHLLFYGPPGLGKTSLAFLIASEMGVSIRVTSGPVIERQ
                     KDLAAILSSLRENDVLFIDEIHRLNPLVEETLYPAMEDYKLDLIIGQGPSAQTYRLKL
                     PRFTLIGATTRAGLLSSPLHNRFGVVQRLDFYDASNLFRIVLRSAKILGVSIVEDGAW
                     EIARRSRGTPRIANRLLRRVRDFAQVLGDGVITREVARGALERLEVDTIGFDEMDRRI
                     LHTIIEKFAGGPVGIETIAVAVGEEKETIEDVYEPFLIQQGFLARTPRGRTVTRLAFD
                     HFNLPRPTESQGELWHG"
BASE COUNT          992 a         1432 c         1372 g         1034 t
ORIGIN      
        1 atccttaacc atcgcaaggc tggctacacc gggaacgtga tggtggcgtg gcctgtgcct
       61 gaagaaaagg tggaggctgt aggacatcaa atggcgcagt ttcgcgcggt gagccactgc
      121 tatcagcgcc ccgcctaccc ggagtggccg tttacgctct ttacgatggt ccatcagaag
      181 agccgtgaag cgtgtgagga gacggtggca gcgatcagtc gcgagacagg gatcagcgac
      241 tatgcgatgc tgtggacgac gcgagaattc aaaaaagttc ggcttaagta ttttaccgaa
      301 gactttgcgg cctgggacga gaaagctata gcccgttagc agacagatcg gtaacaagcc
      361 gtcgcatgga cggacactgc tcccactcct accaccttcc cgttgagctc ttccagcaga
      421 tactcgccta ggcggtcgcc gatgtccact gctctcttcg actgcacgag ctacgcgcct
      481 acgcctggga tcacccgatc ttgcttagtg ttcgggaggg ccagcgccta aagtgcgcta
      541 ctatggaggt gtagttcccc gcttaaagca tgcggggaca ggctcaatcg ctcttaccct
      601 tcgccgtccc ctccgttgtc tcaacggcct tttcggtaat ggcgcccctg gcgatctcaa
      661 ccgtgacatt ttcggcgagc ttgaccttga cccggttgtt ctcaccaaac ttgactattg
      721 taccgtacaa tcctccagta gttacgatct gatcgccggt cttcaggttt tcgagcatat
      781 ccttttgctc tcgcgacttc ttctgttggg ggcgaatcat taagaaataa aagataatga
      841 atattgccac cggagtgatg agggccccga gcatgcctga aggtggactt ccatttgttg
      901 ccccctgcgc atacgctatt cccattttct gacctcctct tggtcttgtt ctgttatgcc
      961 acggtgtaac tactcaagtt gtttaggctg aaggctgtta gtcgttgcgc atggaccgaa
     1021 tcaaggcgtc cagaaccttt tcggtctcca tcattgtcgc gtcgcattcg gaaacgcctg
     1081 gctcatctgt gcgcgttcct acagtctaaa agcctacagt ctatctacct tgtagctacg
     1141 ctacggtttc gtcgcgattc agcgtaccag tttcctcaag gaactttgtc cggaaggcgg
     1201 caaggctccc atcttcgata gcctggcgaa tctgccgcat gagcgtaaga tagaaatgaa
     1261 ggttgtgaag cgtgttcaaa cgcagtccaa ggatctcgcc cgccataaag aggtgccgga
     1321 gataggcgcg ggagaagtgc cggcacgtat aacaatcaca ggcgggatcg agtggcctct
     1381 cgtctgacgc atgcgccgcc cccttaatat tgatccggcc ctggctggta aacaggcttc
     1441 cggtcctgcc gtgccgtgtg ggcattacac agtcgaacat gtctacccct cgcatcacgc
     1501 tttccacgag atcctccggc gttccgaccc ccatcaggta gcgaggccga tcgacaggca
     1561 tgagcggcgt ggtatgagcg acggtctcgt acatgaccgc tttcggctca ccgacggcga
     1621 gaccgcctaa tgcgtagccg tcaaagccga tcttctgcag gtctgttgcg gcctgttccc
     1681 gaagcgccct gtcggtcccg ccctgtaaga tgccgaacaa cgccggttgc ccattgggat
     1741 gggctacccg ggaccgttct gcccacttga gcgtcaaccc tagcgatcgc tgaagatagt
     1801 cggtggtcga gggatagggg gcgcactcgt caagcggcat gatgatatcc gccccaagag
     1861 atttttggac ttcgatggcg agctccggtg agagaaagtg aagtgatccg tcaagatggg
     1921 accgaaagct caccccatcc tccgagatct gtcgtaatgg agccatgctg tatacctgaa
     1981 accccccact gtcggtgagg atcgatcctg accacgccat gaaacgatgc aaaccgccaa
     2041 gctcctcaat gagccgatgg ccgggacgca ggtagaggtg ataggtgtta cacagggcca
     2101 tctgtacccc ctcggtttcc agctcatggg gcgtgagcgc cttgactgcg cctcctgtcc
     2161 cgcatggcat gaatgcaggg gtctccaccg cgccgtgggg cgtcctcagt ctcccacgcc
     2221 gtgcgcctgt tgcagcatcg attttcagca actcgaaggt caccatcgct cctatgaggc
     2281 tatcaacctt cagtcttcag tcagatgttg tccggctacc ttatcacaca atcaacatcg
     2341 cgtcgccgta ggaatagaaa cggtagcgta gggcgatagc ctcacgatac gcagccacga
     2401 cggcttgccg gcctgcgaag gcagagacca gcatgaagag ggtagagcga ggcagatgaa
     2461 agttggtgat cagggcgtcg atacacctat agcgatagcc cgggtagata aagaggtctg
     2521 tgacgccggc cccggacctc acatcgccat cgactgcggc atgctccagg gcccggacgg
     2581 tcgtggtccc gaccgcaatc accctccgcc cctctctctt tgcggccttg accgccaggg
     2641 ccgtctgttc cggaacaatg taacgctccg gctccatctg atgcgcggaa atctcctcca
     2701 cccgaacagg acgaaacgtg ccaggaccga catagagggt gatcggggcc acggcgacac
     2761 cctgccgcgt gagcgtctcg attagttccg gtgtgaagtg gaggccggcc gtgggcgccg
     2821 cgatggcccc ttcatgcttt gcgtataccg tctggtaccg ctcgcgatcc agttccacac
     2881 cccaaatccc cccgtgcccc ccttttgtaa agggggggtg gggggatttc gcaccctgtc
     2941 ttttgatata gggcggaacc ggcatctggc cgaatcgcca caaaaggtca ctgagtgttc
     3001 cgtcatacac gagcttgagc aggtggcgtc cctcaccgcg cttctccatc acctcagccg
     3061 tgatcgtttc atcggccagg gtaaggcggt cgccggctcg aacctgcctg gatggtttga
     3121 ccaaagcctc ccaacagcct gttccggcat gccgtaagcc gggaacagtt tcaacttcac
     3181 gcaagagtaa tacctcgatg agatgccgcc gctcggatcg accgaagagc ctggcgggga
     3241 tcactttggc ctcattgatg accagcagat ctcctggaag gaggtattct gaaagatccc
     3301 ggaagatccg atgttgcagg acgcctgtgt tccgatccag gaccaggaga cgcgagcgat
     3361 cgcgctcggg ggcgggcgcc tgggcaatca gatcaggggg caggatgtag tcaaagtcgg
     3421 ctgtacgcaa agcgagagac acgggaggat tcagctcctg gtagcatcgg cgccgtttac
     3481 cggcggcgaa ataatgaaaa aagtatggtc aggaggatgc tcaacagtat tgaggtggtc
     3541 agggggaaat agaatgaaaa gttctttcgt tggatgtaga tgtcccccgg tagcctccca
     3601 atgaaaggga cctttccgac gaacaggatg agtcccccta cgacggccag aatgaggccg
     3661 aacacaatca acagcttggc gagtgattcc agcccctgca tgtgcgtctt tcgcgtccat
     3721 tgcgtcgtcg cgtcatacgc tatagacgca atctacccat gccataactc gccctgtgac
     3781 tcggtcgggc gggggagatt gaaatgatca aaggccagac gagtgacggt gcggcccctt
     3841 ggggtcctgg ccagaaaccc ttgctggatc aaaaacggct catagacgtc ctcgatcgtt
     3901 tctttttcct ctcctacagc gactgcgatc gtctcgattc ccaccggccc tccggcaaac
     3961 ttttcgatga tggtgtgcag gatccgccga tccatttcgt cgaacccgat ggtgtccacc
     4021 tcgagccgtt ccagcgcgcc ccgtgcaact tcacgggtaa tgacgccatc ccccagcacc
     4081 tgggcgaaat ccctgacgcg ccgcagcagg cgattggcga tgcgaggcgt tccacgggag
     4141 cgtcgcgcga tttcccatgc gccgtcctcg acgatcgata cccccaggat ctttgccgac
     4201 cgcaggacga tcctaaagag gttagacgca tcgtaaaagt ccaagcgctg gaccacgcca
     4261 aaccggttgt gcaaaggcga agacagaagc ccggcgcgag tcgtggcccc gatcaacgtg
     4321 aaacgcggca gcttcagccg gtaggtctgt gcgctcggac cctgaccgat gatcaggtcc
     4381 agtttgtaat cttccattgc cgggtagagg gtctcttcga ccagaggatt cagccggtgg
     4441 atctcgtcaa tgaacaggac gtcattttcc ctgaggctgg agaggattgc cgccaggtct
     4501 ttctgccgtt cgatcactgg tccggaggtg acccggatgc tcacccccat ctccgaggca
     4561 atgagaaagg cgagagaggt cttgccaagc cccggcgggc cgtagaagag caggtggtcc
     4621 aggggctctc ttcgcgcctt ggccccctgt acgtagacct ggaggttcgc cttgaccttt
     4681 tcctggccaa tgtagtcatc ccaggctttt gggcgcagac tgagctcgag atcctgatcc
     4741 tcgtcctgga gctgccgatt cgccacccgt tctggcccgc tctccttctt ctgcttctcc
     4801 gatgtcataa ccgcttcccc gtcctctcgg
//
LOCUS       DATMXI010000332         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_293789, whole genome shotgun sequence.
ACCESSION   DATMXI010000332 DATMXI010000000
VERSION     DATMXI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293789"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(592..1026)
                     /locus_tag="VN444_06485"
     CDS             complement(592..1026)
                     /locus_tag="VN444_06485"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWR21488.1"
                     /translation="MTTLRVLFLCTHNSARSQMAEGFLRALAGDRFDVVSAGTEATRV
                     HPLAIRAMQEIGIDLSGHTSKTIDVFFSQPWDYVITVCDDANERCPVFPGGTTRIHWS
                     FDDPSQATGTDEDKLKKFRRIRDDILTRLRQWLADQEQSPSS"
     gene            complement(1123..>2104)
                     /gene="arsB"
                     /locus_tag="VN444_06490"
     CDS             complement(1123..>2104)
                     /gene="arsB"
                     /locus_tag="VN444_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWR21489.1"
                     /translation="LSRMEVSHVNLPVAILIWLMIYPMMLRIDFSAIRDVGRRPKGIL
                     IVLFVNWLVKPFSMAFLGWLFFKHLFAGIIGPSLGDQYLAGTIILAAAPCTAMVFVWS
                     YLTDGDPAYTLVQVALNDLIMLFAFAPIVIFLLGVSNITVPYDVLLYSVLLYIVIPLA
                     AGAASRTTLLRTHGPAWFEDVFLARLKPVTIVALLATLVIIFAFQGEVILGKLSHIML
                     IAIPLLIQVYFNSGLAYGLAWRLRVPHAIAAPGALIGASNFFELAVATAISLFGLTSG
                     ATLATVVGVLVEVPVMLSVCSICNRTRHWFAEPVTVPVVEPDRPPIGRRA"
BASE COUNT          466 a          581 c          663 g          394 t
ORIGIN      
        1 gttcctggcc accccgtcac tcatcggcgc accgttcttg atagcgggcg gtctgaaggt
       61 cgtctacgac ttactgttgt accgcagttt tcgagagctt aagccgcctg atgaatgagt
      121 acgcatccat aagatgtgag gccgtagcac cccctctcgc ctacgcttgg ttgactaccc
      181 ttcttattga gtcttgcata agtaatggca cacgaattac acgcctaccc gttcggcctg
      241 agcctgtcga aggctgatcc gttcatggtt cgactggctc accacgaacg gaatgaagac
      301 atccgctgtt gcattactta tgcaaggctc aataggcacc gcgatcacat ctctttgaac
      361 acaccctgcg aatgcagctc atcgaggcgt cttggaccgg catcctgctg cgcgaggcaa
      421 ctggcgatca tcgaccggct taggggccgg cagacgaggc gaaacggata gcccactcct
      481 gacatctggg cagcacagcc tatctaacgg tctggcggct ctcagcttgc gccatctcca
      541 agtctccgga ctcaactata ggggggcaag agagaatccg acgacaccag gctatgagga
      601 gggagattgc tcttgatccg caagccattg gcggagccgt gtgaggatgt catcgcggat
      661 gcggcggaac ttcttcagtt tgtcttcatc tgtgccggtg gcctgtgagg ggtcgtcgaa
      721 gctccagtgg atgcgggtcg ttcctccagg gaacaccggg catcgttcgt tggcgtcgtc
      781 gcagaccgtg atcacatagt cccatggctg gctgaaaaat acgtcgatgg tcttggaggt
      841 atgcccgctc agatcgatgc cgatctcctg catggcccgg atcgcaagag ggtggacgcg
      901 tgtggcttca gtcccggcgc tgacgacgtc gaaacgatct cctgcaagcg cgcgtaggaa
      961 tccttcggcc atctggctcc tggccgagtt gtgcgtgcaa agaaaaagga ctcgcagtgt
     1021 ggtcatgtcg ctcctctcag tgtggggcgc tgactgtaga tcgctggcaa gcatccggaa
     1081 gagattcaaa acatagcgaa gcagagatct tgctgaacat ggttaggccc gtcggccgat
     1141 tggcggccgg tccggttcga cgaccggaac cgttacgggt tcggcaaacc agtggcgtgt
     1201 gcgattacag atcgagcaga cggagagcat gacgggcacc tcgacgagga cgccgaccac
     1261 agtggctaat gtcgcgccgg aggtgaggcc aaagagcgag atggcggtgg cgacagccag
     1321 ctcaaagaag ttgctggcgc cgatcagcgc gcccggcgcg gcgatcgcgt gaggcacgcg
     1381 gagccgccac gccaacccgt acgccaaccc cgagttgaag tagacctgga tgagcagagg
     1441 gatcgcgatg agcatgatgt gtgagagctt gcccagaatc acctctccct ggaaggcgaa
     1501 aatgatgacg agagtggcca ggagggccac gatggtgact ggtttgagcc gcgcaagaaa
     1561 gacgtcctcg aaccacgccg ggccgtgagt ccggaggagt gtcgtccgag aggctgctcc
     1621 ggctgcaaga gggatgacga tgtagagtag gaccgagtag agcagcacgt cgtacggcac
     1681 cgtgatgttt gagacgccca ggagaaagat cacgatgggc gcaaaggcga agagcatgat
     1741 caggtcgttc agcgccacct ggaccagcgt gtaggctggg tcgccgtcag tcaggtagct
     1801 ccagacgaag accatggccg tgcacggggc agccgccagg atgatggtcc cagcgaggta
     1861 ctgatctccg agcgagggac cgatgatccc ggcaaagagg tgcttgaaga agagccaacc
     1921 gaggaaggcc atgctaaagg gctttaccag ccagttcaca aagagaacaa tcaggattcc
     1981 cttcggtcgc cgcccgacgt ccctgatggc agagaagtcg atccgcagca tcatcgggta
     2041 gatcatcagc cagatcagga tcgcaaccgg gaggttgacg tgactcacct ccatccggct
     2101 taag
//
LOCUS       DATMXI010000333        10067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_119653, whole genome shotgun sequence.
ACCESSION   DATMXI010000333 DATMXI010000000
VERSION     DATMXI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119653"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="glyQ"
                     /locus_tag="VN444_06495"
     CDS             <1..667
                     /gene="glyQ"
                     /locus_tag="VN444_06495"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWR21490.1"
                     /translation="QHYYQYQVIMKPSPDDIQEIYLDSLRSLGIDPLRHDIRFVEDDW
                     ESPTLGAWGLGWEVWLDGLEITQFTYFQQAGGIDLKPVSVELTYGIERIAMFLQGVDS
                     VYDLTWVEGVTYGDIHHKGEVEWSVHNFEEADVALQLQLFDKYEREGLRLIEKGLVLP
                     AYDYCLKSSHTFNLLDARGAIGVTERTSFIGRVRNLARRCAEAYLKQREEAGFPLMKA
                     WQS"
     gene            692..2761
                     /gene="glyS"
                     /locus_tag="VN444_06500"
     CDS             692..2761
                     /gene="glyS"
                     /locus_tag="VN444_06500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWR21491.1"
                     /translation="MTQDLLFEIGTEEIPSSYITSILKDLEAQACRLFEEQRIAFSGM
                     RTLGTPRRLTLYVERLDQSQGDLVREVVGPSRAVAFDQDGLPTKAALGFAKAQAVSVE
                     NLRVKTLDRGEYVVAVIVERGAHLEEMLPVLLPRLITSLSFPKSMRWGQGTFRFVRPI
                     RWLVALYSGRVIPFEIDGVKSGNKTYGHRFLSRGQIRVRSFQDYIEKLEERYVIVNQH
                     RRRELVTRLAEEAAAKVGGKPVFDDELVEMVTSLVEYPTVVCGRFEHEYLLLPREVIM
                     TPMRKHQRYFPVADDSGKLLPYFVTISNMKAKDMEIIRTGNERVLRARLKDAAFFFKE
                     DRRIQLRERVPQLKGITFQERLGTMLEKVERLTKLVAYLTEQVAPHLVHDACQSAQLC
                     KADLVTTMVKEFPSLQGVIGREYAQLSGEPAVVAQAIEEHYLPRFAGDRLPESLVGAL
                     VGIADRLDTICGCFGIGLIPSGSEDPYALRRLAQGVVQILLSTGIDLPLSQQISKSLE
                     LFGDRLTLPKERVAQEVMGFLAARLQAILIERGVPSDLVEAALSVNAERVSDAGKRAE
                     ALATFRREADFTELAATLKRVINILPKGFSKSVDPRRFVSSAERALHSEATTLRAETE
                     HLVQAGDYVRALQLIAAIRPIVDMFFEEVMVMVEDRDLQENRLAILKEVADLFCGIAN
                     FSKIMAS"
     gene            2883..3536
                     /locus_tag="VN444_06505"
     CDS             2883..3536
                     /locus_tag="VN444_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWR21492.1"
                     /translation="MMDAEILERINRGIILRTGAYLTNDHFVLTSGRHAQEYVEKALA
                     TTEPAFTEGLGDVIAKHFAEEPIDLVLTTGYGASLLGHCVARAHPLRPRFIYAGKQRD
                     GAGVSQVTLPREFRQYFVGSPKVLIVEDIVTTGETVKELIKLVKSLGGTVVGIGTLWR
                     RIRKVNFKFPFFTMVDREFPTYAPKNCPMCRRGIPINQEFLPEPGPQKSHRMRTGSG"
     gene            3838..4509
                     /locus_tag="VN444_06510"
     CDS             3838..4509
                     /locus_tag="VN444_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HWR21493.1"
                     /translation="MPLLPADSGDPGQQAVTLGRETELIAARTLAFASMEDPSPVEKR
                     EKQTLHIEEGFQSGTWHLGVKSGYGDSHKIFAGRESGVNFAPLFFQIGYTVTDVHGPF
                     PVRGSLEVIFEPTFMFISEPAKTFGEGASILLRYNFVTGTRWIPFFEAGIGILHWNLH
                     IPRDLNSQLNFAILGGPGVNYFVTDNLAITGQVGLHHLSNGGRKSPNVGVNSSMYLLG
                     VSYYF"
     gene            complement(4584..4955)
                     /locus_tag="VN444_06515"
     CDS             complement(4584..4955)
                     /locus_tag="VN444_06515"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWR21494.1"
                     /translation="MPKGKECPVISDEEVVKRLEQELPGWYLEGRWIRRKFNTDGWPT
                     TLMLVGAIAYLAEAAWHHPDLEVTWGKVWVKLRTHAAGGITEKDFTLAKQIEQSVLWR
                     PSEDSPLDGTPNKWVRGGSKE"
     gene            complement(5369..7390)
                     /gene="tkt"
                     /locus_tag="VN444_06520"
     CDS             complement(5369..7390)
                     /gene="tkt"
                     /locus_tag="VN444_06520"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWR21495.1"
                     /translation="MGESVKTDQESLDQLCINTIRTLAMDGVQKANSGHPGLPMGAAS
                     MAYVLWERHLRHNPTHPAWPNRDRFILSPGHGCMLLYCLLHLTGYDLLLDELKQFRQW
                     GSRTPGHSEHGVTPGVETTTGPLGQGFGNGVGMAIAERFLAHHFNRPGHLIVDHFTYA
                     IVSDGDLMEGVSSEAASLAGHLGLGKLIYLYDDNRITIDGSTDLAFTENVGQRFEAYG
                     WHVQRVDGDDVHMIDAAISAAQAEHDRPSLIIARTHIAHGSPNKQDTSEAHGSPLGEE
                     EIRLTKEALGWPLEPAFYIPDEALTHFRESLQKGRAQESEWQARFDAYAAAFPELAEE
                     WIKGMSGQLPEGWVEKIPIFTPAGGSLATREASGKVLNAIAQSLPTLIGGSADLAPSN
                     NTHLKGYGDFQRSSPGGRNFHFGVREHAMGSILNGMALHGGVTPYGGTFLVFSDYMRP
                     AIRVAALSHIHVIYVFTHDSIGLGEDGPTHQPIEHLSSLRAMPNLTVIRPADATETAV
                     AWRVALEHRDGPVALALTRQKLPVLDRTKFPPAELLLKGAYILADADQGHPRVILIAT
                     GSEVQVALEAWGRLADEGIPARVVSMPSWELFDRQPEAYRNEVLPPTVTARLAIEAGS
                     PHGWHRYVGLLGGVIGMTRFGASAPIQVLIQQFGFTPEHVVSRAKELLA"
     gene            complement(7507..8565)
                     /gene="gap"
                     /locus_tag="VN444_06525"
     CDS             complement(7507..8565)
                     /gene="gap"
                     /locus_tag="VN444_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWR21496.1"
                     /translation="MAGIAINGFGRIGRLAFRAAWEQKLGLDLVAINDPAGANTDALL
                     LEFDSNYGPFPAKVESDEGSMRVDGKRILVFRERDWTKLRWSDVGVDIVLECSGRGTK
                     RQEAEKHLAGGARRVLISAPAKDEDITIVLGVNHDQYDPTLHRIISNGSCTTNGLAPV
                     AKVLLDAFGIQWGFMSTVHSYTNSQSIHDRGTEDVRESRAAALNIIPTDTGAARALVK
                     VIPELAGRFNGMAFRVPTPTVSVIDLVAEVDRDVTIDTVNAAFREAAKGSLRGILDVC
                     DRPLVSMDFKGNPHSSIVDALMTLVLKDRIVKVVAWYDNEWGYSCRMADLAAYVASQD
                     LKRDQHRLWGVAKDTKTS"
     gene            8881..9369
                     /locus_tag="VN444_06530"
     CDS             8881..9369
                     /locus_tag="VN444_06530"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWR21497.1"
                     /translation="MAAFRIRVDHENLKFSSAHFVLFGNGKCESLHGHNYRAWVELEG
                     SLQPSDYVLDFLTVKPLMKEICERLDHKVMLPTENEDLRIEPGDSVVGVIYGKKQYSF
                     PAEDVLLLPIHNTSAELLARYICGVLVEEIKSRFGGDGVTAIKVGVQESFGQEASYEE
                     VF"
     gene            complement(9366..>10067)
                     /locus_tag="VN444_06535"
     CDS             complement(9366..>10067)
                     /locus_tag="VN444_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWR21498.1"
                     /translation="VHARRGERMAYRPIRSVLLQGADPVALLRAYRDTFESEAVYIAD
                     LDAIMEEGENLAVIAEMAVSESQLQIFVDAGIRSVEQAKSLLDAGASKVIVASESLAS
                     LDTAAQLLAALGTDRALFSVDLKDRAVIWREPSTETRDPCEAATSLMSLGCRETIFLE
                     MDKIGTGGGADAELLGRVTTAAPGMRYIVGGGITSVADLVRLKRAGASGVLLATALHD
                     GTLTRQDLIRVKAGD"
BASE COUNT         2094 a         2873 c         2916 g         2184 t
ORIGIN      
        1 ccagcactac taccagtatc aggtcattat gaagccatcc cctgacgaca ttcaggagat
       61 ctacctggac agtctccgga gtctgggcat tgatccgctt aggcacgata tccgtttcgt
      121 agaggacgac tgggagtcgc cgacccttgg cgcctggggg ctaggctggg aggtctggct
      181 ggacggcttg gagatcaccc agttcaccta tttccagcag gctgggggca tcgacctgaa
      241 accggtctct gtggagttga cctatgggat cgagcggatc gccatgttcc tgcaaggggt
      301 ggacagcgtc tacgacctga cgtgggtcga gggcgtaacc tacggtgaca tccatcacaa
      361 gggcgaggtg gagtggtcgg ttcacaactt cgaggaagcc gatgtggcgc ttcagcttca
      421 gcttttcgac aagtatgagc gggagggtct ccgcctgatc gaaaagggac tggtgctccc
      481 ggcatacgac tactgtctga agtcttctca cacctttaac ctcttggacg ccaggggggc
      541 aataggtgtc actgagcgga ccagcttcat cggtcgcgtc cgaaacctcg cgcgccggtg
      601 cgccgaagcc tatctgaagc agcgtgagga ggcgggcttt ccactcatga aggcgtggca
      661 gtcgtaacca tatagtgcag gacaacccct gatgacccaa gacctgttgt ttgagatcgg
      721 gaccgaggag attccctcca gctacatcac gtcgatcctg aaggatctgg aggcacaggc
      781 atgccgcctt ttcgaagagc agcgcatcgc cttttccggc atgcggacgc tcggcactcc
      841 tcggcgcctt accttgtatg ttgagcggct ggatcagagc cagggtgacc tggtccgtga
      901 ggtggtggga ccatccaggg cggtcgcgtt cgaccaggat ggccttccga ccaaggccgc
      961 gcttggcttt gcgaaggcac aagccgtctc tgtagagaac ctccgtgtta agacgcttga
     1021 ccggggagag tacgtcgttg cggtcatcgt agagcgaggc gcgcatctgg aggagatgct
     1081 gccggttctc ctgccccgcc tgatcacgtc gctctccttt ccgaagtcga tgcggtgggg
     1141 gcaaggcaca ttccggtttg tccgtccgat ccggtggctc gttgccctgt acagtggtcg
     1201 ggtgatcccc tttgaaattg acggagtcaa aagcggaaat aagacgtacg gtcaccgatt
     1261 tctgagccgg ggccagatcc gcgtcagaag cttccaggat tatatcgaaa aactggagga
     1321 gcggtatgtc atcgtgaacc agcaccgtcg ccgggaactg gtgacaaggc tggccgaaga
     1381 ggccgcggcc aaggttggcg ggaagccggt cttcgacgat gagctggtag agatggtgac
     1441 ctccctggtg gaatacccta ccgtagtctg tggccgcttc gagcatgagt atctcctctt
     1501 acctcgtgag gtgatcatga cccccatgcg gaagcatcag cgctactttc cggtggctga
     1561 cgattccggc aagctcctcc cgtatttcgt gacgatctcc aacatgaagg caaaggatat
     1621 ggagatcatc cgtacaggca acgaacgggt cttgagggcg cggctcaagg atgccgcatt
     1681 ttttttcaaa gaggatcgca gaatccagct tcgcgagcga gtgcctcaac tgaaggggat
     1741 tacctttcaa gagcgacttg gcacgatgtt agagaaggtc gagcgtctta ccaagctggt
     1801 cgcctacctg accgagcagg ttgccccgca ccttgtccac gatgcctgtc agtcggctca
     1861 gttgtgcaag gctgacctcg tgaccacgat ggtcaaggag ttcccaagtc tgcaaggggt
     1921 tataggccgt gagtatgccc agctctccgg ggagccggcg gttgtggccc aggcgattga
     1981 ggagcactat ctccctcgct ttgccggcga caggctcccg gaatcactgg ttggcgctct
     2041 cgtaggcatc gccgatcggc tcgacaccat ctgtggctgc ttcggcatcg ggctcatacc
     2101 gagcggatca gaagatccgt acgccctccg gagactcgca caaggggtcg tgcagatcct
     2161 tctcagcaca ggaatcgatc tgccgctctc gcagcagata agcaagagct tggagctctt
     2221 tggtgatcgt ttaactctgc cgaaggagcg tgtagcgcag gaggtcatgg ggtttctggc
     2281 ggccaggctc caggcgatcc tgatagagcg gggcgtgcca agcgatctcg tcgaggctgc
     2341 cctttccgtc aatgcagagc gggtctccga tgccggaaaa cgcgccgagg ccctggctac
     2401 cttcaggcga gaggccgatt ttacggagct ggcagcgacc ctcaaacggg tgatcaatat
     2461 ccttcctaag ggtttctcca aatcggttga tccaaggcgg ttcgtgagca gcgctgagcg
     2521 agcgctccat agcgaggcga caacgctacg ggctgagaca gaacatctgg tccaggccgg
     2581 cgactacgtt cgagctctgc aactcatcgc cgcgatccgc cccattgtgg atatgttctt
     2641 cgaggaggtt atggtgatgg tggaggatcg agatctgcag gagaaccgtt tggccatcct
     2701 gaaggaggtt gcagatctgt tttgcgggat cgcgaatttc agtaagatta tggcttcgta
     2761 agtcttcagt cgccagaagc cttccggcgt aggggagctg cttgctgcgc cctctttggg
     2821 tagggcaagc cctaccccta cgggaacggc tagtagctga acagccataa tgcgtgagga
     2881 ttatgatgga cgcagagatc ctggaacgga tcaaccgggg tatcattctc aggaccggag
     2941 catatctcac aaacgatcac ttcgtattga cctcgggtcg tcacgcccag gagtatgtgg
     3001 agaaggcgtt ggccaccacc gagccggcct tcacggaagg gttaggagac gtgatcgcaa
     3061 aacatttcgc agaggagccg atcgacctgg tccttaccac cggctacggc gcctcgttgc
     3121 ttggccattg cgtagcgcgt gcccatcctt tgcgacctcg cttcatctat gccggcaagc
     3181 aacgcgatgg ggctggcgtc agtcaggtaa ctcttccgag agagtttcgg cagtactttg
     3241 ttggtagccc taaggtactg attgtcgaag acattgtgac cactggtgag actgtcaagg
     3301 agcttattaa actggtcaaa tcattaggcg gaacggtggt cggaatcggg acgctctggc
     3361 gacggattcg aaaggtgaac ttcaagttcc cctttttcac gatggtcgac cgggaattcc
     3421 cgacgtacgc cccgaagaac tgcccgatgt gccgcagagg gatcccgatt aatcaggagt
     3481 ttttaccgga gcctggccca cagaagtctc accggatgag gactggctcc ggctgaagcc
     3541 tgtcctgagc ctgccaaagg ggtcgtgcca ggaatcgagt aacactactc agcgattttc
     3601 gaccgtggaa gctgtgaccg gctcatccct cggatcgagg tggcgggtga gagatcttgg
     3661 aagcggtgag cagcgccact ctaaaggaca tgatcgggat agatgaggcg tgatgcccaa
     3721 gacacttgca ctccagagga gacatctatg gtcaatagat tgaggtgtat accggtttgt
     3781 atctggctgg ctatgttcgc aatcctgcca cctgccatct catgggctga ggcctcaatg
     3841 ccactattgc cggccgattc gggagatcca ggtcaacagg cagtcacgct agggcgagag
     3901 actgagctca ttgccgcccg cacccttgcc tttgccagca tggaagatcc ctctccggtt
     3961 gagaagaggg aaaaacagac gctccacatc gaggaaggat tccaatctgg aacgtggcat
     4021 ttaggggtca agtccggtta tggtgactct cacaaaattt ttgccggaag agagtccggc
     4081 gtcaacttcg ctccgctatt tttccagatt gggtacacag tcaccgatgt ccatggccct
     4141 tttccggttc gtgggagcct ggaagtcatc ttcgagccga ctttcatgtt tatcagcgag
     4201 ccggctaaaa cctttggcga aggcgcaagc atcctcctcc gctacaattt cgtgacgggc
     4261 actcggtgga ttccgttctt cgaggctggt attggtatcc tgcattggaa tcttcacatc
     4321 ccacgggacc tcaacagtca gctcaacttc gccatcctgg gtggtcctgg agtgaattac
     4381 tttgtgacag ataacctggc gattaccgga caagtgggct tacatcacct ctcgaatgga
     4441 ggccgcaaaa gtcccaacgt cggcgtcaat tcgagcatgt atctgctggg ggtctcgtat
     4501 tacttttagg ttcagcgttc agcagtcagc tatcagcaag acacaaaaag ctgaagactg
     4561 accgctgaac gcttctccga gccctactct ttgctgccgc cgcgaaccca tttgtttggt
     4621 gtgccgtcaa gaggagagtc ctcacttggt cgccagagta ccgactgctc gatctgcttg
     4681 gcaagggtaa aatccttttc agtgatcccc ccggcggcat gagtcctcag cttgacccag
     4741 acctttcccc atgtgacctc caggtcaggg tgatgccatg cggcctcagc caggtaggcg
     4801 atcgcgccca ccagcatgag cgtggttggc cagccgtcgg tattgaactt tctcctgatc
     4861 catcgcccct ccaggtacca ccctggcagc tcttgctcca gacgcttaac cacctcctca
     4921 tccgaaatga ccgggcactc tttgcctttt ggcatggacc cctcctcctg tgttgaacca
     4981 ataacgtagc cacatcttac ttgaaatgta gggtacgagc ccccattgga caagcgaatt
     5041 ctagcgctga catcgagccg acgatcgatg atctggagtg cgcccatcgc ggcggtaata
     5101 agccctcata aaaagaacgc gccccaggga tggtcgctga ggcgctttct tttctggtaa
     5161 ctactcaggt gtttaggctg taggctgtta gtcgttgcgc acggaccgaa tcaaggcgct
     5221 tcgtcggcca gttcaaacgc tctgagcttg gtatggtccc gcatggcgcg ttcctacagt
     5281 ctaaaagact acagtctatc tacctgagta gttacctttc ttctactacc ctataccctg
     5341 gtttttattg ttgcccctcc ggtgggattc atgccaggag ctccttagct cgggacacca
     5401 catgctcagg cgtaaagccg aattgttgta tgaggacttg aatcggtgcg gaggctccaa
     5461 atcgtgtcat accgatcacc cctccgagga ggccaacgta acggtgccaa ccgtgcggag
     5521 atcccgcctc gatggcaagg cgggcggtca ctgttggggg aagcacctcg ttacgatacg
     5581 cctccggctg tcgatcaaac aactcccagc tcggcatgct gacgacacgc gcggggatgc
     5641 cttcatcggc cagtcgtccc cacgcctcca gagccacctg cacttccgac ccggtggcta
     5701 tgagaatgac gcgaggatga ccctggtcag catcagccag gatataggct cccttcaaga
     5761 ggagttcggc aggcgggaat ttggttcggt cgagaaccgg gagcttttgt ctggtgaggg
     5821 ccagcgccac gggcccgtca cggtgctcca gggcgacgcg ccaggcgacg gcagtttctg
     5881 tcgcatctgc cgggcgtata actgtaaggt tcggcatggc ccggagggaa gacaggtgct
     5941 cgatcggctg gtgggtgggg ccatcttcac ccaagccgat gctgtcatgg gtgaagacat
     6001 agatcacatg tatgtgcgaa agtgcagcta cccggatggc gggacgcatg taatcggaaa
     6061 agacgaggaa ggtcccacca tacggggtca cgccaccgtg caatgccata ccgttgagaa
     6121 tagaacccat cgcgtgctct cgaacgccaa agtgaaaatt gcgacctccg gggcttgacc
     6181 tctggaagtc gccatatcct ttcaggtggg tgttgttcga cggggccaga tcggccgagc
     6241 cgccgatgag ggtcggcagg gattgggcga tagcattgag caccttgccc gatgcctcac
     6301 gggtcgccag gctccccccg gcaggggtga agatcgggat cttttcgacc cagccctcgg
     6361 ggagttggcc actcatcccc ttgatccact cttcggcaag ttctggaaac gcagcagcat
     6421 aggcatcgaa tcgagcctgc cactctgact cctgggcacg acccttctgc aaggactctc
     6481 gaaagtgagt cagagcctca tcagggatat agaacgcagg ttccagcggc cagcccaaag
     6541 cctcctttgt gagccgaatt tcctcttcac caagaggcga tccgtgagcc tctgacgtgt
     6601 cctgcttatt ggggctaccg tgggcaatgt gagttctggc aatgatgagc gatgggcggt
     6661 catgctcggc ttgggccgcg ctgatcgcgg catcgatcat gtgaacgtcg tccccatcca
     6721 ctcgctgaac atgccaccca taggcttcaa agcgctgtcc aacgttctcg gtgaaggcca
     6781 ggtcggtgct gccatcgatg gtaatccggt tatcgtcgta caggtagatg agtttaccca
     6841 atccaaggtg cccggcaaga gaggccgctt cggaggagac cccctccata aggtccccgt
     6901 cgctcacgat ggcgtaagta aaatggtcaa cgatcagatg tcctggccgg ttaaagtgat
     6961 gggccaaaaa tcgctcggcg atcgccatcc cgacgccatt gccgaaaccc tggccaaggg
     7021 gacccgtcgt cgtctccact cctggtgtca cgccatgctc cgaatgtccg ggggttcggc
     7081 taccccattg gcggaattgc ttgagttcat cgaggaggag atcgtacccg gtcaggtgaa
     7141 ggaggcaata cagaagcatg cagccatggc caggtgaaag gataaaccga tcacgattcg
     7201 gccacgcggg atgagtcgga ttatgtcgga ggtggcgctc ccaaaggacg taggccatgg
     7261 aggcggcccc catgggaagg cccggatgcc ccgagttcgc cttttgaacc ccatccattg
     7321 ccagtgttcg gatggtattg atgcacaact gatcgagcga ttcctgatcg gttttcactg
     7381 attcccccat ataccttcca ccctccaccc tgttttgcag atcgccgctg attataccgg
     7441 cgtccatgtc gtgaaactgt ctgagcgacc ttatgcttcc gcagtgacct cacccgtttt
     7501 cactgtctat gatgtctttg tgtccttcgc aacgccccag agccgatgct gatcacgttt
     7561 gaggtcttga gaggcaacgt aggcggccag gtcggccatc cggcacgaat acccccactc
     7621 gttgtcatac caggccacaa ccttcacaat ccggtccttc aggaccagcg tcatgagggc
     7681 gtccacgatc gaagagtgcg ggtttccctt gaagtccatt gagaccagtg gccggtcaca
     7741 gacatcgagg atcccgcgaa gcgacccctt agccgcttct cggaaggcag cattgaccgt
     7801 atcgatcgtc acgtcacgat caacctccgc gacaagatca atcacggaga ccgtgggcgt
     7861 tgggacccga aacgccatcc cgttgaagcg gccggcgagc tcgggaatca ccttcacaag
     7921 ggcccgcgca gctccggtgt cggtcgggat gatgttgagg gcggcagctc ttgactctcg
     7981 aacgtcttcc gtcccgcgat cgtgaataga ttgcgaattg gtgtagctgt gtaccgtgct
     8041 catgaacccc cattggatcc caaatgcgtc aaggagaacc ttcgccaccg gcgcgaggcc
     8101 gtttgtcgtg catgacccgt tcgagataat ccgatgaagg gtcggatcgt attggtcatg
     8161 gttgacaccg agaacaatgg tgatgtcctc gtcctttgca ggagctgaaa tcagtacccg
     8221 cctcgcccct cctgccaggt gtttttcggc ttcctggcgc ttcgttcccc gcccgctgca
     8281 ctccaacacg atatcgaccc ctacatccga ccagcgaagt ttagtccaat cgcgctcccg
     8341 aaagaccaaa atccgcttcc catctacccg catgcttcct tcgtcgctct ccacctttgc
     8401 agggaagggc ccgtagttgg agtcgaattc cagcaacagc gcatcggtgt tcgcgccggc
     8461 cggatcgttg atggccacca ggtcgaggcc cagcttctgc tcccacgcgg cccgaaacgc
     8521 caaccgaccg attcgcccga atccgttgat agcaatcccc gccatcgatt atcctcctga
     8581 catttctgcg agcccttctt gcggagccgt ctgccgtgct tctctagcat ggagcaggta
     8641 ataaatggct aacaattgca caaggaccag tagatcccta tgaaaggttg aaggtgccac
     8701 catccgtttc atgctacagc gctaccacgt ttccgacctc gcgcggacgg aattttacca
     8761 cttgccccta ttatctgtct agcggttgtt tcaaaacgga tagcaaatct ggcttgacat
     8821 ctggcgcgcc aatgcgatag cttccaacta gctatacgag gcattatgtg aggaggcgta
     8881 atggctgctt ttcgcatcag agttgaccac gagaatctta aattcagttc ggcccacttc
     8941 gtgctcttcg gcaatggcaa gtgtgagtcc cttcatggcc ataactacag ggcctgggta
     9001 gagctggaag ggtcgcttca gccgagcgat tatgtcctcg atttcctcac cgtcaagccg
     9061 ctcatgaaag aaatctgcga gcgactggac cacaaggtca tgctgccgac cgaaaatgag
     9121 gatttgagga tcgagccagg cgattcggtg gtcggggtga tctatggcaa gaagcagtac
     9181 agctttccag cggaagatgt gctgctgctt ccgatccaca acaccagcgc agagttgttg
     9241 gccagataca tctgcggggt gttggtggaa gagatcaaga gccgattcgg gggcgacggt
     9301 gtcactgcga ttaaggttgg cgtacaggaa tcgttcggac aggaagcctc ctacgaggag
     9361 gtcttctaat cgcctgcctt cacgcggatg aggtcttggc gtgtaagggt gccatcgtgc
     9421 agtgcggtgg ccagcaacac cccggaggca ccggcgcgtt tcagccgtac gagatccgca
     9481 accgaagtaa ttccgccacc cacgatgtat ctcatgccag gcgcggccgt cgtaaccctg
     9541 ccgagtagtt ctgcgtctgc cccgccgccc gtgccgattt tatccatctc cagaaagatc
     9601 gtctcacgac acccaagcga catgagggaa gttgctgcct cgcaggggtc tcgcgtttca
     9661 gtcgaaggct ctctccagat gacggcccga tccttgaggt cgacgctaaa aagcgcccta
     9721 tcggtcccta gcgcggcgag gagttgcgcg gctgtgtcaa ggctcgccag cgattccgag
     9781 gcgacgatca ccttactcgc gccagcgtca agcagcgact tcgcctgctc gacgctacga
     9841 atgccggcat caacaaaaat ttggagctgt gactcggaga cagccatctc ggcgatgacg
     9901 gctagattct cgccttcttc catgatggca tccagatcgg cgatgtacac cgcttcactc
     9961 tcgaaggtgt cacggtacgc ccgcagaagg gcaacagggt cggctccttg cagcagaacg
    10021 ctccggatcg gtctgtacgc catccgctct ccgcggcggg catggac
//
LOCUS       DATMXI010000334         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_120674, whole genome shotgun sequence.
ACCESSION   DATMXI010000334 DATMXI010000000
VERSION     DATMXI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120674"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..552
                     /locus_tag="VN444_06540"
     CDS             211..552
                     /locus_tag="VN444_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWR21499.1"
                     /translation="MSVILRTGLLLVLSNVFMTFAWYGHLKNLNDRNWYIAALVSWGI
                     ALFEYLLQVPANRIGYTQLSISQLKIMQEVIALSVFAPFAVLYMNQPFKLDYLWAGLC
                     LLGAVYFIFRT"
     gene            605..1678
                     /gene="mqnC"
                     /locus_tag="VN444_06545"
     CDS             605..1678
                     /gene="mqnC"
                     /locus_tag="VN444_06545"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HWR21500.1"
                     /translation="MAESNGRIEAIAEKVEAGERLSFDDGVELFERATLLELSALADC
                     VRWRLHPEPVVTYVIGRTINYTNICWVQCSFCAFYRMPGSTEGYLLSKEEIFQKIEEL
                     IELGGTEVLLQGGLNPSLKIDYYEDLLASIKARFPVHLHALSTVEIGYISNSSKISLK
                     DTLKRLKAAGLDSIPGAGAELLVDEVRERISPLKESASDWLNLMRIAHGLGIPSTATM
                     MYGVDETLAQRIEHLVKIRDLQDRTGGFTAFIPWSYQPNGDALRGRGGSGYGYLRTVA
                     VSRIMLDNVKHIQASWLTMGPKIGQLSLQYGVNDFGSTVLEENVVRTAVTAQLMPLDE
                     IRRNIADAGFVPKRRNSRYELLE"
     gene            1784..>2631
                     /locus_tag="VN444_06550"
     CDS             1784..>2631
                     /locus_tag="VN444_06550"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWR21501.1"
                     /translation="MSKTVQKLDDYRWLLPQDYKPGMRVPGIIYADEVLMEAIMKDLS
                     LEQVANGAFLPGIVKASLAMPDIHQGYGLPVGGVVATDITDGVVSPGAVGYDINCGVR
                     LLRTELTKEEVRPKLKELVLALFSEIPTGVGSRGRIRLSRKEMDAPLLKGAAWAVKQG
                     YGEPADLTCIESGGCLPDADPDAVSHKALERGSSQLGTLGSGNHFLEVQAVAEIYDPH
                     AAEVLGLFEGQVTVMIHTGSRGLGHQVCTDSLVEMERAVLKYRIDLPDRQLACTPWTS
                     PEAKAYL"
BASE COUNT          553 a          700 c          787 g          591 t
ORIGIN      
        1 tggcaatctc ggcgcaggaa tgcgagattg cttcacttcg ttcgcaatga caactttctg
       61 tcgctgttct tgggtagtgg gctgcggcag acaggtggcc gcgccttatg gactgaatat
      121 actcatggcg ctgcgctcgg cctcttcgtt gagcggatgt tcgtatgaac gcgaatcatc
      181 atttcataat cacatgagag gtatgtctga atgtctgtta ttctccgcac ggggttgttg
      241 ctggtgctgt cgaatgtctt tatgacattt gcctggtatg gtcatctgaa aaacctgaat
      301 gatcgtaact ggtacattgc ggcgttggtc agttggggga tcgcgctgtt cgagtatttg
      361 ctccaagtac cggctaaccg cattgggtat acacaattga gtatctcgca gttgaagatc
      421 atgcaggagg tcattgctct atccgtgttt gctccgtttg ctgttctcta tatgaatcag
      481 ccctttaagc ttgattatct atgggcaggc ctctgtctgc tgggtgcggt ctattttatc
      541 tttcgcacgt gagcgaggtt gagcggacgc gtctgcatca tggtggagcc aaggaatctc
      601 aggtatggct gaatcgaacg gcaggatcga ggcaatcgcg gagaaggttg aggcggggga
      661 gcgactcagc ttcgatgacg gggtcgagct tttcgaacgc gcgacgctgc tggagctgtc
      721 cgccttggct gactgcgtac gctggcgctt gcatcccgag ccggttgtga catatgtcat
      781 cggccgcacc attaactata cgaacatctg ctgggtccaa tgttcgttct gcgccttcta
      841 tcggatgccg ggctctacgg aagggtatct cctgtccaag gaggagatct ttcagaaaat
      901 cgaagagcta atcgagcttg gtgggacgga ggtcctgctg caagggggac tgaatcctag
      961 cctgaagatc gactattacg aggatctgct cgcctcaatc aaggcgcgct ttccggttca
     1021 tctccacgcg ctctctactg tagaaatagg ctatatatca aatagttcca agatatccct
     1081 caaggacacg ttgaagcggc tcaaggcggc gggcctcgat tcgatcccag gggccggggc
     1141 tgaactgctg gtcgatgagg tgagggagcg gatctcgccg ctaaaggaga gcgcatccga
     1201 ctggctgaac ctcatgcgga tcgcccacgg tcttggaata cccagcacag ccacgatgat
     1261 gtacggggta gacgagacgc tggcccagcg cattgagcat cttgtcaaga ttcgagatct
     1321 acaggatcgg accggcggct tcacagcctt catcccctgg agctatcaac ccaatggcga
     1381 cgcgctccga gggcgcggcg gcagtggcta cggctatctt cggactgtgg ccgtgtccag
     1441 gatcatgctg gacaatgtca agcacatcca ggcctcctgg ctgacaatgg gcccaaagat
     1501 cggacagctc tcgctgcagt acggtgtcaa cgacttcggg agcaccgtcc ttgaggagaa
     1561 tgttgtgcgg accgccgtca cggcgcagct tatgcctctc gatgaaatcc gacgaaacat
     1621 tgccgacgcg ggtttcgtcc caaagcgtcg aaactcacgg tatgagcttc tggagtaatc
     1681 tcctttgtag gggcgctgct tgctgcgccc atgagggcag ggcaagccct gcccctacag
     1741 aagaacaacc gaaacgtaca gcacagcagg gcaagggtga acgatgagca agactgtaca
     1801 aaagctcgat gactatcgat ggctactccc gcaagactat aaacccggga tgcgcgttcc
     1861 ggggatcatc tatgccgatg aggtcctgat ggaagcgatc atgaaggacc tctcccttga
     1921 acaggtggcg aatggcgcct tcctgccagg aatcgtcaag gcctcccttg ccatgcccga
     1981 catccatcag gggtacggtc ttccggtcgg tggggtagtg gccaccgaca tcactgatgg
     2041 cgtcgtgtct cccggcgcgg tcggatacga catcaactgt ggggttcgat tacttcgcac
     2101 ggagctgaca aaagaggagg tgcggccaaa gctcaaagag ttggtcctcg ccctgttcag
     2161 cgaaatccca accggcgtcg gatcgcgcgg ccgaatccgt ctgagcagga aggagatgga
     2221 tgcccccctg ctcaaaggcg cagcatgggc tgtcaagcag gggtacggag agccggcaga
     2281 ccttacctgt atcgaatccg gcggctgtct tccggacgct gatccggatg ccgtcagcca
     2341 taaagccctt gagcggggga gtagccagct cggaacgtta ggctctggca atcacttcct
     2401 ggaggtccaa gccgtcgccg agatctacga tccccatgcc gccgaggttc tcggtctgtt
     2461 cgagggccaa gtgaccgtga tgattcatac cggctctcga ggcttggggc atcaagtctg
     2521 cacagattct ctagtcgaga tggagcgggc cgttctcaag tataggatcg atctgccaga
     2581 tcggcagttg gcgtgtacac cctggacctc ccctgaagcc aaggcgtacc t
//
LOCUS       DATMXI010000335         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_251984, whole genome shotgun sequence.
ACCESSION   DATMXI010000335 DATMXI010000000
VERSION     DATMXI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251984"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1028..1462)
                     /locus_tag="VN444_06555"
     CDS             complement(1028..1462)
                     /locus_tag="VN444_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWR21502.1"
                     /translation="MEDREEYVKCFSLDELAKRERSAVPFHVIINPQLSVKDDTSLKF
                     FEACSSLEGFWALVPRARAVQVKCLNEHGETRVIDACGWYARILQHEIDHLHGKLYID
                     RMDSRSFMTSGNRDRYWKGSSIEEIRAALMVAGQCSRQRREN"
     gene            complement(1501..1653)
                     /locus_tag="VN444_06560"
     CDS             complement(1501..1653)
                     /locus_tag="VN444_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWR21503.1"
                     /translation="MKLLKIFQVGEPVLRQQARPLSTEEIKSDKIQQLIESMRETMRY
                     GGDIGG"
     gene            complement(1735..2208)
                     /locus_tag="VN444_06565"
     CDS             complement(1735..2208)
                     /locus_tag="VN444_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21504.1"
                     /translation="MNIVLCGHVYRLCRRVAILATFALWLLCLPPVHSMAEGATHIDI
                     VRDRFQQLLDQWGYHEWWSMWEQGTSQSRAAISKDAFTQKMDSSLWRLACCDKRLRNL
                     QITPASSRHVVVSATLLFETKGSPRSVQERPYLINLNFYLEEEQWRIDLSGLGHP"
     gene            complement(2205..3380)
                     /locus_tag="VN444_06570"
     CDS             complement(2205..3380)
                     /locus_tag="VN444_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21505.1"
                     /translation="MIPRQLLDPLIQALLEAPAVCLLGPRQAGKTTLALEVGKRLGGL
                     YLDLESEQDRAKLAEPEAYLERHLDTLVILDEVHRTPNLFPVLRGLIDRARRNGQGNG
                     RYLLLGSAALGLLRQSGESLAGRVRFLELMPLTVREPTGRDAATLWLRGGFPESLLAQ
                     SDEQSLRWRRDFIRTYLERDIPQVGPRIAAETLRRFWTMLAHRHGAPLNVAELARSLG
                     VDTKTAGRYVDLLVDLLLARRLAPWRANVGKRLIKAPRLYLRDSGLTHALLGIADLEA
                     LLAHPVAGASWEGFVIENVIASAPEGIEAYYYGTSGGAEIDLLLHLPGGRMWAIEIKR
                     SLAPRPERGFHAACADLNPVRRFVVYPGVERFPLGQGVEALSLTDLTAELHGMRKAR"
     gene            complement(3503..>3942)
                     /locus_tag="VN444_06575"
     CDS             complement(3503..>3942)
                     /locus_tag="VN444_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWR21506.1"
                     /translation="MDVPERVFSEPVNEEVARFVGIETILRGHAQSVSQGLATVEVPG
                     WTLEVAEPLTPGERVLVCLRPEEITLFPRGAILTTSSARNQLYGRVIRHLPAGSTYRV
                     TIDCGALIVAAVTRQSWEQLGLTDGAEVVAAFKATAPHIIHCA"
BASE COUNT          853 a         1202 c         1086 g          801 t
ORIGIN      
        1 gatcagagct gtcagacacg tgtccatgtc cacctgctga accacgacct tcacgtctcg
       61 ctccttctct gttgcagtcc cgggacctcc gtctccctct gtctctactg tgtaagacga
      121 ttgcacggta tcaaacctga catgtctata accgtccgga taccgaggag ccaccaaaac
      181 caccgacacc tcttcttggc cgtgagcaac cgactgtact cgatgctgac cgcgcgtaag
      241 gcagatggca tccagatgcg gcattctcca tggtgttggg attcttacac taccttaatc
      301 tgttaacaaa aaacagaagc gcgctttacg taccgccggt acgaaaagcg cgcttgttca
      361 cgccttgata gcaccagata tcctccaggg ggtcatctgg aactcttcgt gagaagatcc
      421 ccggtccaac ctaatcccca ttctcagggg attgcgaacc ggtgcctgta gcccgcattt
      481 gcgagggagt taacttataa cgtctccccg tccctcgtca accacaaaac aggtaatcga
      541 aaagaatttg aacctcgata agccatttca gatttgtcca ctatcactca cgcaatcatg
      601 taatatccgt tgatatcagt ggattgcaaa cacggatttt gcactaactt cttttaaata
      661 cccccttttt acctcaaaga acacattata agtggaatag atgtggccat ggagagacgc
      721 tcatgtgcat acagtcttcg tacgacatcg cgcaagcgca caagcgcgag gggtgaacgc
      781 aatcttcagg tggcgccttc tcccctttgc tcaacatggc cgccctgttt ctagacaagg
      841 ttgtacgttc gtagaactga gaattgatct gccggtcgag tttccgcacc gaccagccgc
      901 cgcagggctt cgatcttata aacgcgcctg gtattcttgt tcttcgccgt aatcagccag
      961 acataggcag gccacggcaa ggagaagcgc gttgatgtcg ttttcagaaa gaattgcacg
     1021 ggctgcctca attctctcga cgctgtcgag agcattggcc ggcaaccata agagccgcgc
     1081 gtatctcctc aatagaacta cccttccagt atcggtcacg gttgccactt gtcataaacg
     1141 agcgtgaatc catccgatcg atatagagct tgccgtgcaa atgatcaatc tcatgctgga
     1201 ggatccgtgc ataccaaccg catgcatcaa tcaccctcgt ttcaccatgt tcgttcaggc
     1261 acttcacttg gacggctcta gcgcgcggta caagcgccca aaatccttcc aggctcgaac
     1321 atgcctcaaa gaatttgagc gatgtatcgt ctttgacgga aagttgggga ttgataatga
     1381 catgaaacgg gaccgctgat cgctcacgct tcgcaagttc atcgagagaa aaacacttca
     1441 catattcctc tcggtcctcg ataacagcaa gctgtaggga ttcgccgacc tgtggggcgg
     1501 ctagcccccg atgtccccgc cataacgcat cgtctcacgc attgactcta taagctgctg
     1561 gatcttatca cttttgattt cttcagtgga aagcggacga gcctgctgac gcagcaccgg
     1621 ctctcccact tgaaagatct tcagtagctt cataagtccg cctccgtaga gttgctgcga
     1681 tggatgtgaa agtgatcatg ggtgaggctg cgacgcaatg tatcatgtcg cgtatcatgg
     1741 atggcccaga ccggagagat cgatgcgcca ctgctcctcc tccaggtaga aattgagatt
     1801 gatcaggtat ggccgctcct gtacagacct gggagagccc ttagtttcga acagcagtgt
     1861 ggccgacacg acgacgtgac gagacgatgc cggtgtgatc tggagattgc gaaggcgctt
     1921 atcgcagcat gcaaggcgcc aaagactact gtccattttt tgcgtgaatg cgtcttttga
     1981 gatggcggcg cggctctgac tcgtgccttg ctcccacatc gaccaccatt cgtgatagcc
     2041 ccactgatca agcaactgct gaaagcggtc ccggacgatg tcgatgtggg ttgcgccctc
     2101 tgccatggag tggaccggcg gaagacacag cagccacagt gcgaatgtgg cgagtatggc
     2161 aacacgtcgg cataggcgat aaacatgccc gcacaacacg atgttcatcg tgccttcctc
     2221 atgccgtgga gttcagcggt aagatcggta agcgacaacg cttcgacgcc ctgcccgagc
     2281 gggaatcgct ccacgcccgg atacaccacg aaccggcgta caggattcag gtccgcgcag
     2341 gccgcatgaa acccccgctc gggacgcggc gcgaggctgc gtttgatctc gatcgcccac
     2401 atccggccgc cgggcagatg aagcagcaga tcgatctccg cgccaccgct cgtgccgtag
     2461 taatacgcct cgatgccttc tggcgcggac gcgatcacgt tctcgatgac aaacccctcc
     2521 cagctcgctc cggccacggg gtgcgcaagc agcgcttcca gatcggcgat acccagcagg
     2581 gcgtgagtca ggccactgtc gcgcaggtac aggcgcgggg ccttaatcag acgcttgccc
     2641 acgttggcgc gccagggcgc cagccgccgg gccagcaaca aatccactag cagatccacg
     2701 taacgcccag ccgtcttggt atctaccccc aggctgcgcg cgagctcagc cacattgagc
     2761 ggggcaccgt ggcggtgcgc gagcatggtc cagaaccggc gcagcgtttc cgccgcgatg
     2821 cgcggcccga cctgggggat atcccgctcc agatacgtgc ggatgaaatc ccgccgccag
     2881 cgtaggctct gttcgtcgct ctgggcgagc aggctctccg gaaagccgcc cctcagccat
     2941 agcgtggccg catccctgcc tgtgggctcc cgtacggtga gtggcatcag ttcaaggaaa
     3001 cggacccgcc cggcaaggga ttcgcccgac tgccgcagca ggcccagagc ggccgagccc
     3061 agcagcaggt atcgcccgtt gccctggccg ttgcgccgag cgcgatcgat gagcccgcgc
     3121 aggaccggaa agagattcgg cgtgcggtgg acctcgtcga ggatgacgag cgtatccaga
     3181 tggcgctcaa gataggcttc cggctcggcc aacttcgcgc ggtcctgttc cgactccagg
     3241 tccagataca acccgcccag tcgtttccct acctccagcg ccagcgtagt cttacccgct
     3301 tgccgcgggc ccagcagaca gaccgccggg gcctcgagca gggcctgaat cagtggatcg
     3361 aggagttgtc gtggtatcat gcttgtaagt atggagtatt agtccatgat ttacaaggat
     3421 aatcattgct acggcagcga ccccgaactg ttcgagggaa tcaccaccaa accggacact
     3481 acggggtttg cagaacaggt tcctatgcgc agtggatgat gtggggggcg gtggccttga
     3541 atgcggcaac aacctcggcc ccgtccgtga ggccgagctg ctcccacgac tgccgggtga
     3601 ctgccgccac gatcagggcg ccgcagtcaa tcgtcacccg gtaggtggag ccggctggca
     3661 gatggcggat cacccgcccg tagagctgat tccgtgcgct ggaggtggtc aggattgccc
     3721 cgcgagggaa gagcgtaatc tcctccggcc tgaggcagac cagtacgcgc tccccgggcg
     3781 tcaacggttc ggccacctcc agggtccacc ccggaacctc gacagtggcg agcccctgac
     3841 tgacggactg cgcatggccc cgaagaatcg tttcaatgcc gacgaagcgg gctacctcct
     3901 cgttgacagg ctcggaaaac acccgctcag gcacatccat ct
//
LOCUS       DATMXI010000336         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_298326, whole genome shotgun sequence.
ACCESSION   DATMXI010000336 DATMXI010000000
VERSION     DATMXI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298326"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VN444_06580"
     CDS             complement(<1..464)
                     /locus_tag="VN444_06580"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21507.1"
                     /translation="MQRRTRERSSKKGETTPAPAKRSRLRRRVIVLFCSLAAALTVIS
                     GRLFSLQVYQYADLVEAAKRQSSDRISSVSRRGTIYDRNGRDLAISLGTSSIAARPSQ
                     IVDPKRAAAALSVALSLPAEKILERLQAGKPFVWIKRSVSTEEAQAVARLML"
     gene            complement(558..941)
                     /locus_tag="VN444_06585"
     CDS             complement(558..941)
                     /locus_tag="VN444_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21508.1"
                     /translation="MSAQQLHDTRRKVLVVDDDSALRELLEVHLERVGFDVRTAQDGR
                     AGLHALGESHFDLILTDYRMPAMTGLEMATYIRRSDTITPIILITGDSSTLDPEVIAQ
                     AGITRVLPKPLKINELLNECSLAAV"
     gene            complement(1071..1463)
                     /locus_tag="VN444_06590"
     CDS             complement(1071..1463)
                     /locus_tag="VN444_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWR21509.1"
                     /translation="MREQSIAALTSVGRDRVGSLLKPRVDQIRRLDLLPSLLLGSLVL
                     FGVLCYVWQPIQVVRLGYQVEGLAGERAGLIRQQKELRLEVARLKSLRRVEEIARQQL
                     GLTSPKPGQVIAVESVPSKITGNELKEY"
     gene            complement(1477..2376)
                     /gene="rsmH"
                     /locus_tag="VN444_06595"
     CDS             complement(1477..2376)
                     /gene="rsmH"
                     /locus_tag="VN444_06595"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWR21510.1"
                     /translation="MGEQHVPVLLTETLDAMRPHAGGRYLDVTVGLGGHAEALLIESA
                     PAGHLYGIDRDAETLVLTKARLRQFGDRVELFQGDFAMLGAIATENGWGPFDGILFDL
                     GFSSFQLDDASRGFSFMKDGPLDMRMDRQGSGMSAADLLARLSERELARILQDYSEER
                     WARRIAARIVQDRQTQPLNSTAQLARLVAAAVPRRAWPRRIHVATRTFQALRIAVNDE
                     LAKLIRGLQDAIELLATGGRICIISFHSLEDRIVKETLRGWARSDPPRVHLLTKRPVV
                     PTEREIEVNVRARSAKLRAAERC"
     gene            complement(2452..2901)
                     /gene="mraZ"
                     /locus_tag="VN444_06600"
     CDS             complement(2452..2901)
                     /gene="mraZ"
                     /locus_tag="VN444_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWR21511.1"
                     /translation="MFRGSFEHAIDDKGRLSIPARYREILKRRRERELVLVDLLFDAC
                     IAAYPIKTWQQIEQNLLSKGNSDKKFREYARLISARAVESTVDSQGRILIPPQLREKA
                     DLRRDVVIVGVLDKIEIWNRDRWASFCAQERDPEDYAGKLAELGIRV"
BASE COUNT          631 a          911 c          772 g          621 t
ORIGIN      
        1 ttcagcatga ggcgcgcgac agcctgcgcc tcctccgtgg agactgatcg cttgatccag
       61 acaaacggct tccccgcttg gagtcgctca aggatcttct cggcaggcag gctgagcgcg
      121 acagataggg ctgcggcggc acgcttcggg tccactattt gggacggccg agcggcgatt
      181 gaggaagtcc ccaggctaat ggccagatcc cgaccattgc gatcgtaaat cgttccgcgc
      241 cgggagaccg atgaaatccg gtccgaggac tgccgcttag cagcctcgac caggtctgcg
      301 tattgataca cctggagaga aaagagtcgg ccggaaatga ccgtgagggc tgcagcaagt
      361 gaacagaaca gcacgatcac gcgacgccgt aaccggctac gcttcgctgg cgcgggtgtg
      421 gtctctcctt ttttggatga tctctcgcga gtacgacgct gcatcggcat accacatctt
      481 ccaaagacta ctcggttaca aaccagtcca aagcataagg aatttcaacg ctccccagga
      541 ggccattgcc ggttatttta gacagcagcg agtgaacact cattcaagag ctcattgatc
      601 ttcagaggtt tcggcagtac ccgggtaatc ccggcctgcg ctataacctc ggggtcgagc
      661 gtagaggagt cgccggtgat gaggatgata ggggtaatcg tatcggacct ccggatatag
      721 gtcgccatct cgaggccggt cattgcaggc atgcggtaat ccgtcagaat gagatcgaag
      781 tgggactcgc ctaaggcgtg cagcccagcg cgaccgtcct gcgcggtgcg tacatcgaaa
      841 cccacccgct caagatggac ctccaacagc tcccgcaagg cactatcgtc atccactacc
      901 aggaccttcc gtctcgtatc atgtaactgt tgtgcgctca ctattgctcc cattttcttt
      961 ggcaaactgg tgtgtacgta acatgccatg aatcttgact caatcctgga cgcgctggaa
     1021 tcctttaaaa atggtttgta cttgcaaccc cgtccaaagc atgaggaatt tcaatactct
     1081 ttcagctcgt tgccggttat tttggacggc actgattcga ccgcgatcac ttgaccaggc
     1141 ttaggactgg tgagtccaag ctgctggcgg gcgatctcct ccacacggcg gagcgacttg
     1201 agccgggcga cctcaaggcg cagctctttt tgttgtcgaa tgaggccggc gcgctccccc
     1261 gccaggccct ctacctggta cccgagtcga accacctgaa tgggctgcca cacgtaacac
     1321 aagactccga ataggacgag gcttcccagc agcagcgacg gaagcaggtc gagccttcgg
     1381 atctgatcaa ctcgtggttt cagtaaactg cctacccgat ctcgtccaac cgacgtcaac
     1441 gcggcgatac tttgttctct cacttcatcc cctcctctag caccgttccg cggctcgaag
     1501 ctttgcgctg cgagcacgaa cattaacctc aatctctcgc tctgttggga caaccggccg
     1561 tttggttaga agatggacac gcggcggatc cgaacgggcc caccctcgaa gcgtttcctt
     1621 cacaatccga tcctcaaggg aatgaaaact gatgatgcag atccttcctc cagtagcaag
     1681 cagctcgatg gcatcctgta ggccacgtat aagcttggcc aactcatcat ttacggcaat
     1741 acgtaacgct tggaaggttc gtgtcgcgac atggatccgg cgtggccagg cacgccttgg
     1801 tacggccgca gccaccaaac gcgcgagctg agcggtagag ttcagtggct gcgtctgccg
     1861 gtcttgtacg atccgcgccg ctatccgcct cgcccaacgc tcctcactgt agtcctgcag
     1921 gattctcgcg agttcgcgct ccgagagcct ggctagtaga tctgccgccg acatcccact
     1981 cccctgacga tccatccgca tatccagcgg cccgtccttc atgaagctga atcccctgga
     2041 cgcatcgtca agttgaaagg atgaaaatcc cagatcaaac aggatgccat cgaaagggcc
     2101 ccaaccattc tcagtagcga tggcacctag catggcgaag tcaccctgga ataattcgac
     2161 tcgatccccg aactgcctga ggcgcgcctt agtcaagacc aaggtctcgg catcgcgatc
     2221 aatcccgtac agatgacccg ccggcgcgct ctcaataagg agggcttccg cgtgtcctcc
     2281 caatcccacg gtcacatcca ggtagcgacc acccgcatgc ggccgcatgg catccaacgt
     2341 ctcagtaagt aagaccggga cgtgctgttc cccgatggtt acgccagccg cacccacatc
     2401 ctgcactctg taccccgcac acccgctttc acgggggcag cccttttgca cctatacccg
     2461 gatcccaagt tccgccagct tgccggcgta atcctcaggg tcccgctcct gcgcgcaaaa
     2521 ggacgcccag cgatctctat tccagatttc gatcttgtcc aataccccaa caatcacaac
     2581 atcccggcgt agatccgcct tttccctgag ttgcggagga atcagaattc gcccctgact
     2641 gtcaacagtc gactcgaccg cacgggccga gatgaggcga gcgtattccc ggaacttttt
     2701 atctgagttt cccttactca ggagattctg ttcgatctgc tgccaggttt tgatcgggta
     2761 ggctgcgatg caggcgtcaa agaggagatc gaccaggacg agctcacgct ctcggcgccg
     2821 tttgaggatc tcgcgatatc gggccgggat actgagccgc cccttgtcat caatcgcgtg
     2881 ctcgaaactt ccgcgaaaca tgggctcgca ccgcttttct atggtacttt atacc
//
LOCUS       DATMXI010000337         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_292091, whole genome shotgun sequence.
ACCESSION   DATMXI010000337 DATMXI010000000
VERSION     DATMXI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292091"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VN444_06605"
     CDS             <1..1989
                     /locus_tag="VN444_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HWR21512.1"
                     /translation="DGTVRDNPKSNSSWIALTSKSRDLLRGVQLLLLNLGIKSQILDR
                     SRPARVSLFPPHTTPDGTRTYGSDGVLFELGIFGQSRDRFRREIGFLDLKQQRLENVR
                     YRGFYTQRVSDIVVAVEPSGIRKVYDLTEPQSHSMIANGFVVHQCGEQPLGPNDACNL
                     GSVNLARFYLPSIPADARETERIDWVGLEQVVRTAVRFLDDVIDVNPYPLQDITEEVR
                     NNRRIGLGVMGWADLLLELGVPYDSDEAVTLGDEIMGFIRRIGHDASEKLAEARGPFP
                     RWSRSIYKGKKPLRNSTVTTVAPTGTISIIVGCSSGIEPIFAVAFSHIVGDRHLTFVN
                     PIFEEVAKRRGFYSEELMRRVAERGTVRGLEGVPEDVQRVFVTAHEIEPARHVKMQAA
                     FQKQTDNGVSKTINLPNSATPEDIAKAYMMAYELGCLGITVFRDGCKGTQVLHLGTGA
                     KPALSSVEGSALSPAEEPAVRDAEVAAKQEGAPQAIPIVLKTGLEAALKVKPRPRTMK
                     GVTYRAETPLGTAFVTVNQNGEGEPFEVFAGVGKAGSDTSAVSEAIGRLISMVLRLPS
                     PMSPRERVGQIVNQLSGIGGRRQMGFGKDRVRSLPDAIAQVLAEHIGLTEPGVQEMQA
                     ALGKTTEKAGDMCPDCGSATLVYEEGCQKCYSCGFSEC"
     gene            2066..>2194
                     /locus_tag="VN444_06610"
     CDS             2066..>2194
                     /locus_tag="VN444_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWR21513.1"
                     /translation="MSIKSDAWIKRMAVEYQMIAPFEEGQVRNGVISYGLSSYGYDI"
BASE COUNT          439 a          605 c          718 g          432 t
ORIGIN      
        1 gacggtacgg tccgtgacaa cccgaagagt aacagctcgt ggatcgcgtt aacgtcgaag
       61 agtcgcgatc tgctgcgcgg cgttcagctt ctcctgttga acctgggcat caagagccag
      121 atcctcgatc ggtcacgtcc cgctcgggta agcctcttcc ccccacatac aacgcctgat
      181 ggcacacgta catacggctc agatggggtc ctgttcgaac tcggcatctt cggacaatcc
      241 cgtgatcgct tccgccgcga aatcggcttc ctcgacctga agcaacagcg tctggagaac
      301 gttcggtatc gtgggttcta tacccagagg gtcagcgata tcgtagttgc ggtggagccg
      361 agtggcatac ggaaagtcta tgaccttaca gagccccaga gtcactcgat gatcgcaaat
      421 gggtttgtgg tccaccaatg cggtgagcag ccgcttggac ccaatgacgc ctgcaacctg
      481 ggttcggtca atctggccag attttacctc ccctcgattc cggcggatgc cagagagacg
      541 gagcgaatcg attgggttgg cctggagcag gtggtgcgga cggcagtccg cttcctggac
      601 gacgtgatcg acgtgaaccc ctatccgctc caggacatta ccgaggaggt ccgaaacaac
      661 cggcggatcg gtctcggggt tatgggctgg gctgatctgc tgcttgagct tggcgttccc
      721 tacgacagcg acgaggcggt cacgcttggc gacgagatca tgggcttcat tcggcgaatc
      781 ggccatgacg cctccgagaa gctggcagag gcgcgagggc cgtttccgcg gtggtcgcgg
      841 agcatttaca agggcaagaa acctcttcgc aactcgaccg tcacaaccgt tgcgcctacc
      901 gggacgatca gcatcattgt aggttgctct tccgggatcg agccgatctt tgctgtcgcg
      961 ttcagccaca tcgtaggaga ccggcacctg accttcgtga atccgatctt cgaggaggtt
     1021 gcgaagcggc gcggcttcta cagcgaagag ctgatgcggc gggtggccga gcgggggacg
     1081 gttcgcggac ttgaaggcgt gccggaggac gttcaacggg tcttcgtcac ggcccacgag
     1141 atcgagcctg cccggcacgt caaaatgcag gcggccttcc agaagcagac cgataatggc
     1201 gtctccaaga cgatcaatct gccaaactcg gcgacgccgg aggatatcgc taaggcctat
     1261 atgatggcgt atgagcttgg atgtctcggg attacggtat tccgtgatgg ctgcaagggt
     1321 acccaggtct tgcatctcgg caccggtgcc aagcctgccc tgagcagtgt cgaagggtca
     1381 gcgctgagcc cggccgaaga gcctgccgta cgcgatgccg aagtggcggc gaagcaggag
     1441 ggggcgccgc aagcgatccc aatcgttttg aagacaggcc tggaggcagc gctcaaggtc
     1501 aagccgagac cgcggaccat gaagggcgtc acgtataggg cggagacgcc gcttgggacc
     1561 gccttcgtta ccgtcaacca gaacggcgaa ggtgagccgt ttgaggtctt cgccggcgtc
     1621 gggaaggccg ggagcgacac ctctgcggtg tctgaggcga tcggtcgact catctcgatg
     1681 gtcctcaggc tgccgtcgcc gatgtcgccc agggagcggg tcgggcagat cgtgaaccaa
     1741 ctctccggca ttggcggccg gcgacagatg gggttcggca aggatcgggt ccggtcgctg
     1801 ccggatgcca tcgcccaggt cctggccgag catatcggtc tgaccgagcc cggcgttcaa
     1861 gagatgcagg cagctcttgg taagacgacc gagaaagcgg gcgacatgtg ccctgactgt
     1921 ggctcggcca ccctcgtcta tgaagagggt tgccaaaagt gttattcctg cgggtttagt
     1981 gaatgttagg tcgccggtgt aatagctact caatcttagg tgatttataa aggtggagac
     2041 gtgaagtctc gcaggaggac gtcctatgtc gattaaatcg gatgcgtgga tcaagcggat
     2101 ggcggtggag tatcagatga ttgccccgtt cgaggagggg caggtcagga acggggtgat
     2161 ctcctacggc ctctcgtcgt atgggtacga catc
//
LOCUS       DATMXI010000338         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_299242, whole genome shotgun sequence.
ACCESSION   DATMXI010000338 DATMXI010000000
VERSION     DATMXI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299242"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..944)
                     /locus_tag="VN444_06615"
     CDS             complement(162..944)
                     /locus_tag="VN444_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWR21514.1"
                     /translation="MNIRNVGLILHRELKSYFSSLTVYVVIVMFLLMTGYFFYNLLAT
                     FSIVSFQAQGDPMLARQYQLLNINETVIRPLFGSISIILLMMMPLLTMRLLAEEKKTG
                     TIELLLTFPVDDIDVVLGKYLACLVVLLTMISLTVTYPLLIIFLGEPEVIPIVTGYLG
                     LALLGAAAISLGIFTSSLTENQIVSASISFGMLFFFWLISYSAPLVSAGLGQVLSYLS
                     INEHIASMSKGVLDSEDIIYYLCFIVLFLFLTLRSLETNRWR"
     gene            complement(941..1873)
                     /locus_tag="VN444_06620"
     CDS             complement(941..1873)
                     /locus_tag="VN444_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21515.1"
                     /translation="MIEVENLTKEYGEFRAIDDISFTVEKGEILGFLGPNGAGKTTVM
                     RILTCFFPPTRGKAMVAGYNCFEDSLNVRKRIGYLPERVPLYEDMKVADYLRFVAAIK
                     GMPTKDVNRKAAQVMDDCGLGEVSNKLISAISRGYRQRVGIAQALVNDPEVLILDEPT
                     VGLDPKQIKEIRKLIKELAGKHTVILSTHILPEVSILCGRVIIINKGKLVAVDKPENL
                     TSASARKTYLRIGGDVLRVEQELSLVGGIKNFMREQAAGDVNAFIVAFEDEKRGRQGI
                     QAMIERNNWDLIEMRPIEASLEDIFIKLTTREEA"
     gene            2090..2932
                     /locus_tag="VN444_06625"
     CDS             2090..2932
                     /locus_tag="VN444_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21516.1"
                     /translation="MKAERRLGVSAFALLGLLSISGCDGGGGSTSGNAGTSQELTIGR
                     MKVSVMPEYDDPSVLVIYDGRFADASRYPIKTSFLIPKGSVISDACSLSHEGQHFCQL
                     YKTANRGNYDEVSLILPYPNFYLSFHTPRLDTRVETKSIDYRVKANNAVRTLEVEIQQ
                     PLRSAAFKVVPPTGATTLQDNGSVSLIRGFNHVAYKLDGVAAHQEHGFLISYTKSDPN
                     PSVDIKYTSNAMREPQGSTTSYESQRNIKTVIYVLFGTGVLGLFAILAWYLRSRKRVQ
                     GKQP"
     gene            2971..3372
                     /locus_tag="VN444_06630"
     CDS             2971..3372
                     /locus_tag="VN444_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HWR21517.1"
                     /translation="MLTLLISIMASSGAWALTVNEVAKDLACPCECPLILEDCNMTCG
                     LEWKNQIGEFINKGMTKQQIVDYFIAKYGDEARITPTQRINGKIYQYTRSFDEVDWGL
                     LWSALAVWAFLMFFGVYIGVRKVFFEQAPDA"
BASE COUNT          800 a          984 c          963 g          746 t
ORIGIN      
        1 aaaatagaag taaaaaaaca gcaggagtgc aagcagtcca agccatatca atgttaccgg
       61 caggttgccc atcgtgccgg taatcgcaaa gacgatcccc cctgccgcga tcaggaagcc
      121 cgacaagatg cccagaggca cgatccatcc cttgcgtctc atcatctcca cctattcgtt
      181 tccaggcttc tcagggtgag gaacaggaag aggacgatga agcaaaggta atagatgatg
      241 tcctccgaat ccaggacgcc tttcgacatg ctggcgatgt gttcgttgat cgacaggtaa
      301 ctgaggacct ggcccaaacc cgcagagacc agcggtgcgg agtagcttat cagccagaag
      361 aaaaacagca tgccaaagga tatcgaggcg gacacgatct gattctccgt cagggaggac
      421 gtgaatatcc ccagggaaat ggcggccgcc ccgagcagcg ccagccccag gtagccggtg
      481 acgatgggta tgacctccgg ctctccgagg aagatgatga gcaagggata ggtcacggtc
      541 agcgatatca tggtcagcaa gaccaccaga caggccaggt atttgcccag caccacatcg
      601 atgtcatcga ccggaaatgt caggagcagc tcgatcgtcc cggtcttctt ctcctccgcg
      661 aggagccgca tggtcagcaa gggcatcatc atcagcagga tgatgctgat gctcccgaaa
      721 aggggccgta tcacggtttc gttgatgttg agaagctggt attgccgggc cagcatgggg
      781 tcgccctggg cctggaagct gacgatggag aaagtggcca gcaggttgta gaagaaataa
      841 cccgtcatca acaggaacat cactatgacg acatagacgg tcaaggaact gaaataggat
      901 ttcaattccc gatggaggat caggccgaca ttcctgatat tcatgcctct tcccgggtgg
      961 tcagcttgat gaagatgtct tccagacttg cctcgatcgg gcgcatttca atgaggtccc
     1021 agttgtttct ttcgatcatg gcctgtatgc cttgccttcc gcgtttttcg tcctcgaacg
     1081 caacaatgaa ggcattcacg tcgccggccg cctgttcccg catgaagttc ttgatgcccc
     1141 cgacgagtga cagctcctgc tcgacacgca gtacatcgcc gccgatgcgc agataggttt
     1201 tcctggcgct tgccgacgta aggttctccg gcttgtccac tgccaccagt ttccccttgt
     1261 tgatgatgat caccctgccg cacagaatgc tgacttccgg gaggatatgg gtgctgagaa
     1321 tgaccgtgtg cttgccggcc agttccttga tgagttttct gatctccttg atctgctttg
     1381 ggtcgagccc caccgtgggc tcgtcaagaa tcagcacctc gggatcgttg accagtgcct
     1441 gggcaatccc gaccctctgc cggtatcccc tggagatcgc gctgatcaac ttgttggaaa
     1501 cctcccccag cccgcaatcg tccatgacct gtgcggcctt gcgattgacg tccttggtgg
     1561 gcattccctt gatcgccgcg acaaagcgaa ggtaatcggc aaccttcatg tcctcgtaga
     1621 gcggaacccg ttccggcaga tagccgatcc gcttgcgtac gttcaaggaa tcttcaaagc
     1681 agttgtagcc ggcgaccatg gccttgcccc gggtcggcgg gaagaagcaa gtcagaatgc
     1741 gcatgacagt ggtcttcccc gccccgttcg ggcccaggaa accaagaatc tcgcccttct
     1801 ctaccgtgaa actgatatcg tcaatggcgc gaaactcgcc atattccttg gtcaaatttt
     1861 cgacttcaat cattgcttcc gcacagagtg aatatggggg cgaatacttg tttttaatta
     1921 taaatgtata agcgaatcta gtgtaggaaa agcgcggttc cgctgaggga gatcacaatg
     1981 ctggcgcccc tccaggactg tggaacgttg cttggcggcg gtatagaatt cgccttggaa
     2041 aattgaaggg gaactgcgaa tgtccgatca acgagagggt tcttgagcca tgaaagccga
     2101 acgacgtctc ggcgtctccg cgtttgcgct tctcggcttg ctctccatct cgggttgcga
     2161 tggaggcggc ggaagcacat ccgggaacgc gggaacttcc caggaactca ccataggccg
     2221 catgaaagtt tcggtcatgc ccgagtacga tgaccccagc gtactggtga tctacgacgg
     2281 caggttcgcg gacgcatcgc gctatcccat caagaccagc tttctcatcc ccaaaggctc
     2341 ggtaatcagc gacgcttgca gtctctccca tgagggccag cacttctgcc agctttacaa
     2401 gaccgccaat cgcggcaact acgacgaagt cagcctgata ctgccttatc ccaacttcta
     2461 tctcagtttt catacgccac ggctcgacac cagggtcgaa acgaaatcga tcgattatcg
     2521 ggtcaaggcg aacaatgccg tcagaacgct ggaagtcgag attcagcagc ccctgagaag
     2581 cgccgcgttc aaggttgttc ccccgacggg cgccacgacc ctgcaggaca atggttccgt
     2641 gagcctgatc cgggggttca atcatgtggc ctacaagctc gatggggtcg ccgcgcacca
     2701 ggaacatgga ttcttgatca gttataccaa gagcgatccc aatccctcgg tcgacatcaa
     2761 gtacacctcc aacgccatgc gggaaccgca aggcagcacc acatcctatg aaagccagag
     2821 aaacatcaag acggtaatct atgtgctgtt cggaaccggg gtactcggcc tctttgcgat
     2881 cctggcctgg tatctgcgct cgcgaaagcg cgtgcaggga aaacagccgt gaaaccacgg
     2941 acctcctggc gcggagggcg gctatgggct ttgctcacgc tgctgatctc gatcatggcc
     3001 agttccggtg cctgggcgct gacggtcaac gaggtcgcga aagacctcgc atgtccctgc
     3061 gaatgcccgc tgatcctcga agactgcaac atgacctgcg gactcgaatg gaagaaccag
     3121 atcggcgaat tcatcaacaa gggcatgacc aagcagcaga tcgtcgacta tttcattgcc
     3181 aagtatggtg acgaggcccg cattaccccc acgcagagaa tcaatggcaa gatttatcaa
     3241 tacacgagaa gtttcgatga ggtcgattgg gggttgctgt ggtccgcctt ggcggtgtgg
     3301 gccttcctga tgttcttcgg cgtctacatt ggcgtgagga aggtgttctt tgaacaggcg
     3361 ccggatgcgt gagcggcgca tccgaacggc tattgagagg cgcttcccaa gagaccaaca
     3421 catgagcttt attgaaacac cgcccggtcg ccgctttccc tggtatgttc gtctctttct
     3481 tgccaatcag cgc
//
LOCUS       DATMXI010000339         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_262468, whole genome shotgun sequence.
ACCESSION   DATMXI010000339 DATMXI010000000
VERSION     DATMXI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262468"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VN444_06635"
     CDS             <1..362
                     /locus_tag="VN444_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat-containing
                     protein"
                     /protein_id="HWR21518.1"
                     /translation="SGARAYVANFVANTVSVIDTATNTVTTTITVEQRPDFLAASPDG
                     GSVYVPNQNSNTVSVIATATNSVVDSPITVGTAPTGVAFTPVSIPVIPTLSDGALLLL
                     LGAMVATLALRIARSPL"
     gene            complement(469..1518)
                     /gene="selD"
                     /locus_tag="VN444_06640"
     CDS             complement(469..1518)
                     /gene="selD"
                     /locus_tag="VN444_06640"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1468..1470),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWR21519.1"
                     /translation="MSDQQIRLTSLSHGAGUACKISPADLAQVLGQLPQSNDPNILVG
                     TETSDDAAVYRLDSRQAIVQTVDYITPVVDDPYSCGLIAAANSLSDIYAMGATPLFAL
                     NIVGFPVGSLPLSILGEIFRGGADKVREAGASIIGGHSTDDPEPKYGLVVTGLIDPAK
                     IFKNSTAIIGDDLVLTKPLGIGIITTGIKRDKVTQSTIDAAIALMTTLNKGASEAMVA
                     VGAHACTDVTGFGLLGHLHEMTAGSKVGARVSLSKVPVLSETWDLIREGICPGGTRRN
                     YESLQGAIVWNPEIRQEAQLILCDAQTSGGLLIAVPKDRTAALIQRLRERETPASALI
                     GEIIEDPDGRIWVEP"
     gene            complement(1550..2134)
                     /locus_tag="VN444_06645"
     CDS             complement(1550..2134)
                     /locus_tag="VN444_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWR21520.1"
                     /translation="MLERLREWDEVGFHLINGSLRNPIFDLLMPFVSNKWNFAIPVAV
                     LLIYVLLFRPRRARLVVIFAIAVILLTDATSHILKDLFQRPRPFHPLGDATHLVSFSF
                     PSNHASNMFALATFLSYNYSRSGLLCFPLAALVGYSRIYVGSHYPFDVLGGALWGVLI
                     GLLGAAAVQRLMRSAWVQYTGKSRDNKDHPACGP"
     gene            complement(2164..>2805)
                     /locus_tag="VN444_06650"
     CDS             complement(2164..>2805)
                     /locus_tag="VN444_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWR21521.1"
                     /translation="FQMTRRDEASLASILVERLSGFVALAIIACIAMIPAYTYLNDPV
                     VVWLVVIAATGVIGIVAGLLSDRLQALFFRVLNGVGLGRFHDGVHRLYEAVQQYWTHR
                     RTLLLALGLSLILQSLVITIFYLISLALNLSVPLRYFFLFFPLICVISMLPISIAGLG
                     LREGSAVYFFSKVGLDSASALSLSLLFFAVTALCSGLGGIAFLVGHSQHRMDP"
BASE COUNT          655 a          803 c          793 g          554 t
ORIGIN      
        1 cgagcggcgc ccgcgcctac gtcgccaact ttgtagccaa caccgtctcg gtcatcgata
       61 cggccaccaa cacggttacc accacgatca cggtggagca gaggcctgac tttctggctg
      121 cgagtcctga cgggggatcg gtgtacgtgc ccaatcaaaa ttcgaatacc gtctcggtta
      181 tcgccacggc tacgaatagt gtggttgaca gccctatcac ggtaggcacg gcccctactg
      241 gggtcgcctt cacccctgta agtatcccag ttataccaac cctctcggat ggcgccctgt
      301 tactcctatt gggcgccatg gtcgccacgc tcgccctgcg gatcgctcgt agtccattgt
      361 gagccataac catcccttcg ctcgcaaggc atgaatcaag ctatttacga tcttctcggt
      421 tcctacccct aaaagccttc agccttcagc ctatctacct gttatcgatt acggctccac
      481 ccagatccgg ccatccggat cctcgatgat ttcaccgatc agggccgagg ccggtgtctc
      541 acgttcccgc aggcgctgaa tcaaggccgc agttctgtcc ttcggcaccg cgatcaggag
      601 gccgccggag gtctgggcat cgcacaagat aagctgggcc tcttgccgaa tctctggatt
      661 ccagacaatg gccccttgca gagattcata gttccgtcgc gtgccgcctg ggcagattcc
      721 ctctcggatt agatcccacg tctcagaaag aacgggaacc tttgagagcg acacccgcgc
      781 tcccaccttg ctacctgctg tcatctcgtg gaggtggcct aacagcccga agcctgtgac
      841 gtcagtacag gcgtgtgcgc ctaccgccac catagcctcc gatgcgcctt tattcagcgt
      901 agtcatcagc gcgatagcag catcaattgt tgattgcgtg accttgtcgc gcttgatccc
      961 tgtggtaatg atcccgatac cgagaggctt ggtcaacacc agatcatccc cgatgatggc
     1021 ggtcgagttt ttgaagatct tcgcggggtc gatcagcccg gtcacgacga ggccatattt
     1081 tggctccggg tcatcggtgc tgtgccctcc gataattgag gctcctgctt cgcgaacctt
     1141 atcggcgccg ccacgaaaaa tctcccccag gatactcagc ggaagcgatc ccactggaaa
     1201 accgacgatg ttgagagcga acagtggagt ggcgcccata gcatagatgt cgctcagaga
     1261 gttggcggca gcgatcaggc cacagctata ggggtcgtcc actacgggtg tgatgtagtc
     1321 tacggtttgg acgatcgcct gcctactgtc cagtcgatag acagcggcat catcggaggt
     1381 ctctgtcccc acaaggatgt tcgggtcgtt agattgtgga agttggccca gcacctgggc
     1441 cagatcagca gggctgatct tacacgctca gccagcccca tgtgataagg acgtgagccg
     1501 aatctgttga tcactcatca ttcatctcct caaagcacca ctggaaggtt tatggcccgc
     1561 aggccggatg gtccttgttg tctctcgact tgcctgtata ctgaacccat gcactccgca
     1621 tgaggcgctg gactgctgcg gcgccaagca gtccaatcag gactccccag agcgctccac
     1681 ctagcacatc aaacggataa tgcgagccta cgtagatcct ggaataccca accagagcgg
     1741 cgagagggaa acagagcagc cctgatcgtg agtaattata ggagagaaat gtagccaaag
     1801 caaacatgtt gctggcatgg tttgaaggaa aggagaacga aacgagatgc gtggcatccc
     1861 cgagaggatg aaatggcctg ggccgttgga acagatcctt gaggatgtgg ctggttgcat
     1921 cggtgagtag aatcacagcg atggcgaaaa tgactacgag acgagctcgt cttggacgga
     1981 agagtaacac gtaaataagc agaacagcca ccgggatggc gaagttccat ttattactga
     2041 cgaacggcat caggagatcg aagatcgggt tccgcaggct tccgttaatc aggtggaatc
     2101 cgacttcatc ccactcgcgc agtctctcta gcacggcaga acagacatgg ttggttccgc
     2161 ggtttacggg tccatccggt gctgagagtg accgaccaga aacgcgatcc cccccaaccc
     2221 gctacagagg gctgtgacag cgaaaaatag taaggacagg ctgagtgcgc tagccgaatc
     2281 caggccgacc ttggagaaga agtagacagc gctgccctca cggaggccta ggcccgcgat
     2341 agagattggc agcatcgaga tgacgcagat caacgggaaa aacagaaaaa aatagcggag
     2401 ggggacggat aggttgagcg ctagcgagat caggtagaag atcgtgatga ccagcgactg
     2461 caagatcaac gagagcccca gcgcgagcag gagggtccgc cggtgggtcc agtactgttg
     2521 gaccgcctcg tacagtctat ggaccccatc gtggaaccgg cctagcccca ccccattcag
     2581 taccctgaag aacagcgcct ggagccgatc gctcagcaaa ccggcaacga taccgatgac
     2641 ccccgtagcc gctatgacaa ccaaccatac gacgacgggg tcgttcagat aggtgtaggc
     2701 tgggatcatg gcgatgcagg cgatgatggc aagggcgaca aagccggaga gccgctcgac
     2761 aagaatagag gccagtgagg cttcgtctcg acgggtcatc tggaa
//
LOCUS       DATMXI010000340         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_28652, whole genome shotgun sequence.
ACCESSION   DATMXI010000340 DATMXI010000000
VERSION     DATMXI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28652"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /gene="dnaE"
                     /locus_tag="VN444_06655"
     CDS             complement(<1..1258)
                     /gene="dnaE"
                     /locus_tag="VN444_06655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWR21522.1"
                     /translation="MHHSDFVHLHVHSQYSLLDGACSLEKLVAKAKEYRMPALAVTDH
                     GNLFGAIDFYTIAMKEGVKPIIGCEVYIAPGSRFERSNQDSGYEGASHITLLAKDQVG
                     YKNLIKLVSAGYLEGFYYKPRIDRELFAQHCQGLIALSGCLNSEAAKALLDGDEARAR
                     ETVGWYMEALGRENYFLEVQDHGMAEQKKVAEGIVRLAKAYELPIVGTNDLHYPSKED
                     ARAHEVLLCIQTGKTIQDKDRWRFSTDQFYFKSAAEMKQLFVELPEAVKNTIGVAERC
                     NLQLQFGELRLPKYQAPEGYTIETYLAHLALEGLKVRYPDANAEVEARLKYELEVIHK
                     TGFAGYFLVVWDFIKFAKDRGISVGPGRGSAAASLVAYCLGITNIDPLRYGLIFERFL
                     NPERISMPDMDIDFSDDRRDEVIEYVT"
     gene            complement(1322..1492)
                     /locus_tag="VN444_06660"
     CDS             complement(1322..1492)
                     /locus_tag="VN444_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21523.1"
                     /translation="MTSRQSLKRAYEIEVFGEALYAMAAYLTRRPDHRRKWETLRQLE
                     AQTRDQLLEVRC"
     gene            complement(1596..1916)
                     /locus_tag="VN444_06665"
     CDS             complement(1596..1916)
                     /locus_tag="VN444_06665"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWR21524.1"
                     /translation="MRILLDACVPRRLANELPGHEVRTVPEMGWADLDDGPLLDAMAG
                     LFDVLVTVDKSLPKQQRVRTRPFGVVVLRARTNRLADLLPLVSALRAAVEELHPGEVR
                     ELAG"
     gene            complement(1935..2171)
                     /locus_tag="VN444_06670"
     CDS             complement(1935..2171)
                     /locus_tag="VN444_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWR21525.1"
                     /translation="MMAASRIPEPLITTSPDRLSGTPIFAGTRVPIQTLIDYLEAGDT
                     LDEFLKDFPSVSREHAIAVLELAKSALLTKAVAA"
BASE COUNT          457 a          675 c          620 g          455 t
ORIGIN      
        1 tggtcacata ctcgatcacc tcatcccgcc ggtcgtcgct gaagtcgatg tccatatcag
       61 gcatgctgat ccgttcgggg ttcaggaacc gttcgaaaat caggccgtac cggagcggat
      121 cgatgttggt gatgccgagg cagtaggcaa ccagcgaggc ggccgcggaa ccacggccag
      181 ggccgaccga gatcccacga tccttggcaa acttgatgaa gtcccagacc accaggaagt
      241 agccggcaaa gccggtctta tggatgacct ccaactcgta cttcaggcgc gcctcgacct
      301 cggcattcgc gtccgggtat cggactttta gtccctccaa ggccagatgg gccagatagg
      361 tctcgatcgt gtacccctct ggcgcctggt acttgggcag gcggagctct ccgaattgga
      421 gctggaggtt gcaccgttca gccacaccga tggtgttctt gaccgcctcc ggcagttcca
      481 cgaagagctg cttcatctct gctgccgatt tgaagtagaa ctgatcggtg gaaaagcgcc
      541 aacgatcctt gtcctgaatc gtcttcccgg tctgaatgca gagcaggacc tcatgggcac
      601 gcgcatcttc tttactggga taatggaggt cgttggtccc gacgatcggc agctcatagg
      661 ctttggcgag ccgcacgatg ccctcagcga cctttttctg ctcggccatt ccgtggtcct
      721 gaacctccaa aaagtagttc tcgcgcccca gcgcctccat gtaccagccg acggtctccc
      781 tcgcccgagc ctcgtcgcca tccagcaatg ccttggccgc ctcactgttc aggcagccgg
      841 agagggcaat cagtccttga caatgctggg caaacagctc cctgtcgatt cgcggtttgt
      901 agtagaaccc ctccagatag ccagccgaaa ccagcttgat gagattcttg tagccgacct
      961 ggtccttggc cagcagggtg atgtggctgg cgccctcgta gccgctgtcc tgattggacc
     1021 tctcaaaacg gctgccgggg gcaatgtaca cctcacagcc gatgattggc ttcacccctt
     1081 ccttcatcgc gatggtataa aagtcgatcg caccgaagag gttaccgtga tccgtgacag
     1141 ccagggcagg catccgatac tccttcgcct tggccaccag cttctccagg gagcacgccc
     1201 catccaacag gctgtactgg ctgtgaacgt gcaggtgcac aaaatcagaa tggtgcatga
     1261 atcctccgaa aaaccagggt gtagggtata gagttcgggt taggaagtgc agcttgccgg
     1321 ttcagcatcg gacctccagg agctgatctc tggtctgcgc ttcgagttgc cgcaacgtct
     1381 cccacttgcg tcgatggtca ggccggcgtg tcagatacgc cgccatggcg tacaacgcct
     1441 ccccgaatac ctcgatctcg tatgctcgct tcagactctg ccgagatgtc attgtcgcaa
     1501 ttctcgacgc cgcataacag tttgcatcag cggctgcggc gggcaagcac gccatggatc
     1561 ccggcggcat aagccgctgc cggctgcctg cgatgttagc ctgccagttc acgaacctcg
     1621 ccaggatgta actcctccac tgctgcacga agcgccgaaa ccaaagggag aaggtcggcc
     1681 agccggtttg ttctcgcccg aagaacaacg actccaaagg gtcgagtgcg tacccgctgc
     1741 tgcttcggca ggctcttatc gaccgtcact aaaacgtcga acaatcctgc cattgcatcc
     1801 agcaggggac catcatccag atccgcccag cccatctcgg gaacagttcg cacttcatgt
     1861 cctggcaact cgttagcgag ccgcctcgga acacaagcat cgagtaagat gcgcacgtct
     1921 ggagccgccc agaattacgc cgcgaccgct ttggtcagga gggcggactt ggctagctcg
     1981 agaacggcaa tggcgtgttc acgggaaacg ctggggaagt ccttcagaaa ctcatcaagg
     2041 gtatccccgg cttccagata atcgatgaga gtctggatag gcactctggt gccggcaaag
     2101 atgggggtgc cactcagtcg atccggcgat gttgtgatca atggctcggg aatacgcgag
     2161 gctgccatca tagagcttcc ttgccgacag gccgaccggt cttacac
//
LOCUS       DATMXI010000341         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_137337, whole genome shotgun sequence.
ACCESSION   DATMXI010000341 DATMXI010000000
VERSION     DATMXI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137337"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            622..>2246
                     /locus_tag="VN444_06675"
     CDS             622..>2246
                     /locus_tag="VN444_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase"
                     /protein_id="HWR21526.1"
                     /translation="MSPNPNAAVTKAKANRTFAQALLIKKYWWLHALIVTAISVIGLI
                     ALGVWTYASAPPLVNFVSSKTGEVVIPEWEMNRGKQVFFLKGLMTYGSFWGDGAERGP
                     DFTAEALHRTSVSMGTYYENEIAKTRPVTQDDKDMIKSRIEREIHTNLYDSGAGVIRL
                     NDAQVFAYQELITHYTRMFTDQTYVEAFQNGRIKSYISNPADIKALAGYFFWGGWVSG
                     ANRPGEVYSYTHNWPYDPAVGNFPTYATYIWSFLSIFVLFLGTMLVLYVYGEMKTLPG
                     EPFNGRDWSLTTVDLENKGDAYVRPTQRATYKFFAFAMILFLVQILAGIIGAEDFIGG
                     GPVDAMLGAVGLTIPFSVARGWHLIVQIYWFFMAWVGYTLFFLPRISKVPNGQRALIN
                     LLFTLCVIVGAGALFGIYLGHTGFFATDEMAYWFGSQGWEFLELGRFWHILMLFSFCL
                     WVFIIFRAVKPWITSQNMWSVPAWLFYGSGIMVLFLFFGMFMTPQQNFAISDYWRWMN
                     IHMWVEVTFEVFTTCIVGYMLVQMGLVNRAMAER"
BASE COUNT          494 a          582 c          651 g          519 t
ORIGIN      
        1 acgtgaacga gatcacgcgg cgagagcccg cgactcgcgc gggggctgga ccggcgccgg
       61 gagccacaaa ggaaccccca aggcagcgtg atagccgcga tcgaacagat tgagccgatt
      121 gcgggattta tgggacctgc aggaaaatag taaaacacct ccttgtataa atatggtcaa
      181 ttgagtcaat cgctgaagtt gcggcctcat ggaaggcaaa atagtcaagc gtagtgaata
      241 tgcatcatca aattaggtca tttgccggat ttacgagacc cattcggata gtatactaaa
      301 gcagtgtgag actatgtata tgcggcgtga gttgactcga ttacaggcgg tgcaaagact
      361 accggggatt gcgatatcgc cacagcggta gtcttcccca taaactccac aatagcgaaa
      421 ggaggtgaat cggcgcgcag agcaagtaga gtaacgggtc accgactggc cgaccaggcg
      481 tgtgctgtct agtttacgcc atgcaggccg ttcgcgaagc ccgtatgtcc ccggcgggcg
      541 gggcaacagc aacaaatgtg ggactgcaat aacaacagga gagtggccac ctaaatcata
      601 gggagagcca cgggaggaaa aatgagtcct aaccctaatg cagccgttac taaggcgaag
      661 gccaaccgga cctttgcaca agccctatta attaagaaat attggtggct tcatgctttg
      721 atcgtcacgg ccatcagcgt cataggtctt attgctctcg gcgtctggac ttatgcgagc
      781 gctcccccgc tggtaaattt tgtgtcgtcc aaaactgggg aagttgtgat cccagagtgg
      841 gagatgaatc gcggaaagca agtgtttttc ctgaagggcc tgatgacgta tggctccttc
      901 tggggagacg gagctgagcg cggaccggat ttcaccgctg aggctctcca tcgcacgtcc
      961 gtctccatgg gcacgtatta tgagaatgag atcgcgaaga cgcggcccgt gacccaagac
     1021 gacaaggaca tgatcaagtc caggatcgag agggagatcc atacgaacct gtacgactca
     1081 ggggccggcg ttattcgtct taacgacgcc caggtttttg cgtaccagga gttgatcact
     1141 cactacaccc ggatgtttac ggatcaaacc tacgtagaag catttcagaa tggcagaatt
     1201 aagagctaca tcagcaaccc agcagacatc aaggccttag cgggctactt cttctggggc
     1261 ggctgggttt ccggggcgaa tcgaccgggt gaggtctaca gctacaccca caactggcca
     1321 tacgatccag cggtcggtaa cttcccgacg tacgcgacgt atatctggag cttcctttcg
     1381 atcttcgtgc tgttcctcgg caccatgctc gtcctgtacg tctacgggga gatgaagacc
     1441 ctgccaggcg agccgttcaa tgggcgggat tggtcgctga ctacggttga ccttgaaaac
     1501 aagggcgacg cctatgttcg tcccacccag cgtgcaacgt ataagttttt cgcttttgcc
     1561 atgatcctgt tcctagtcca gatcctggca ggtatcatcg gcgcagaaga tttcatcggc
     1621 ggtggaccag ttgatgcgat gttgggcgcg gttgggctca ccattccctt cagcgttgcc
     1681 agaggctggc atctgatcgt ccagatctac tggttcttca tggcctgggt cggctacacc
     1741 ctcttcttcc tgcccaggat ttcgaaggtc ccgaacggac agcgggccct catcaacctg
     1801 ctctttaccc tgtgtgtcat cgtgggcgca ggcgcgctgt ttggtatcta tctcgggcat
     1861 acgggctttt ttgccactga cgagatggcc tactggttcg ggagccaggg ttgggagttc
     1921 ctggagctcg ggcggttctg gcatattctc atgctgttct cgttctgtct ctgggtcttc
     1981 atcatcttcc gcgccgtgaa gccgtggatc acgagccaga atatgtggtc cgtgccggct
     2041 tggctgttct acggcagcgg gatcatggtg ctgttcctgt tcttcgggat gttcatgacc
     2101 cctcagcaga acttcgccat ttcggactac tggcggtgga tgaacatcca tatgtgggtt
     2161 gaggtcacct tcgaagtctt caccacctgt atcgttgggt acatgctggt gcagatgggt
     2221 ctggtgaacc gggccatggc cgagcg
//
LOCUS       DATMXI010000342         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_317188, whole genome shotgun sequence.
ACCESSION   DATMXI010000342 DATMXI010000000
VERSION     DATMXI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317188"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /gene="glgB"
                     /locus_tag="VN444_06680"
     CDS             <1..1246
                     /gene="glgB"
                     /locus_tag="VN444_06680"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HWR21527.1"
                     /translation="FMYFVDHLHQRGIGVILDWVPSHFPRDPHGLVYFDGTCLYEHED
                     PRKGEHREWGTLIFNYGRKEVDNFLLGNALFWLERYHLDGLRVDAVASMLYLDYSRQP
                     GEWVPNIYGGHENLEAVAFLKRFNELVYERHPGVMTIAEESTAWPQVSRPTYVGGLGF
                     GLKWNMGWMHDMLGYMTLDPVHRGYHHNNLTFGLLYAFSENFVLPLSHDEVVHEKGSL
                     LGKMSGDTWQKFANLRCFYVFMYGHPGKKLLFMGGEFGQWREWDHDQSLDWHLPTEGP
                     HHQGLQRLVRDLNQLYRCQSALHEVDCDPHGFEWIDCHDWQGSIVSFLRRAKVPDDFV
                     VVVCNFTPVPRHGYRIGVPTGGYYVEVLNSDAEIYGGSNLGNGGGIAAEPISEHGRPF
                     SLLLTIPPLSGLILKPATGAAL"
     gene            1243..>2111
                     /locus_tag="VN444_06685"
     CDS             1243..>2111
                     /locus_tag="VN444_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21528.1"
                     /translation="MTDRPAEQPPSTRLRTGSVQDKLRFLLVLHNHQPLGNFDEVIQT
                     LTDRAYRPLLEAIYSRPTLKFTLHLSGPLLLWLERRAPDYLDLIGELVQRGRLELLSG
                     GLYEPILAAIPHEDRIAQITLMSERIRSRFGVRPRGLWLTERIWDSAIIPSLVGAGVD
                     YVLMDDRAFLTSGFEAGRLHDYYLTEAEGEPLAIFPIDKRLRYLIPFRPPEEIEAHLR
                     WIARIGGRLAIAADDGEKFGGWPGTAKWVYDDGWLKGFLDLLEGADDWLVTQTVSEAL
                     EDIRPAGLCYLPT"
BASE COUNT          390 a          617 c          644 g          460 t
ORIGIN      
        1 cttcatgtac ttcgtagatc acctgcacca gcgcgggatc ggcgtgatcc ttgactgggt
       61 cccctcccat ttcccccgcg acccgcacgg gttggtctac ttcgacggga cctgcctcta
      121 tgagcatgaa gatcctcgta agggcgaaca tcgcgagtgg ggtacgctca tcttcaatta
      181 cggccgaaag gaagtggaca acttcctgct gggcaatgcg ctcttctggc ttgaacgcta
      241 tcacctggac ggtttgcggg tggatgcggt ggcctcgatg ctctacctgg actactcgcg
      301 tcagccaggc gagtgggttc ctaacatcta cggggggcat gagaatctgg aggcggtcgc
      361 cttcctgaag cgcttcaacg agctggtcta cgagcgccat cccggcgtga tgaccattgc
      421 tgaggagtcg accgcatggc cccaggtctc gcgcccaacc tacgtgggcg gactcggctt
      481 cgggttgaag tggaacatgg ggtggatgca tgacatgctg ggctacatga cgctcgaccc
      541 ggtccatcga ggctaccacc acaacaacct gacctttggt ctgctctatg ctttcagtga
      601 gaacttcgtc ctgccccttt cccacgacga ggtagtacac gagaaaggct cactcctcgg
      661 caagatgtcg ggtgatacct ggcagaagtt cgccaaccta cgctgcttct atgtctttat
      721 gtatggacat cccggcaaga agcttctgtt catgggaggc gagtttggtc aatggcgcga
      781 gtgggatcac gaccagagtc tggattggca cctcccgaca gaaggtcctc accatcaagg
      841 gctgcagagg ctcgtgcggg acctgaacca gctctaccga tgccagtcgg ctctacacga
      901 ggtcgattgt gatccgcacg gattcgagtg gatcgactgc catgactggc aggggagcat
      961 cgtctcgttc ttgcgtcgcg ccaaggttcc ggatgacttc gtggtcgtgg tctgtaactt
     1021 cacccccgtt ccgcgacacg gctatcgcat cggcgtaccg accgggggct actatgtgga
     1081 ggtcctgaac tcagacgcag agatctacgg tggcagcaac cttgggaatg gtggcggcat
     1141 tgcggcagag ccgatctcgg aacatggacg gccgttctcg ctgcttctca ccattcctcc
     1201 attgagcggg ttgatcctta agcctgctac aggtgccgcg ctatgacaga ccgacctgct
     1261 gagcagcccc cttcgactag gctcaggaca ggctccgttc aggacaagct ccgttttctg
     1321 cttgttctac acaaccatca gcctctgggt aattttgatg aggtgatcca gacgctgacg
     1381 gacagggcct accgacctct tcttgaggcc atctatagca gacctacgct taaattcacc
     1441 ctgcacctga gcgggccgct tctgctatgg ctggagcgcc gagcgccgga ttacctcgac
     1501 cttatcggtg aactggtcca gcgcggccga cttgaactcc tgtccggagg gttatatgag
     1561 ccgatcctgg cggccattcc gcacgaggac cgcatcgccc agatcacgct gatgagcgag
     1621 cgtatccggt ctcgtttcgg tgtccgcccg cgcggtctgt ggctgacgga acggatctgg
     1681 gacagcgcga tcatcccgtc ccttgtgggg gcaggggtcg attatgtcct gatggatgac
     1741 cgggcgtttc tcacgtcagg atttgaggcc ggtcgactgc acgactatta tctcaccgaa
     1801 gccgagggcg agccgttggc catcttcccg atcgataagc ggctccggta cctgatcccg
     1861 tttcgcccgc cggaggagat tgaggcgcac ctgcgttgga tcgcacggat aggcgggcgg
     1921 ctggccatcg ctgccgacga tggtgaaaag ttcggcggct ggcccgggac ggcaaagtgg
     1981 gtctacgacg acggatggct gaaggggttc ctggacctgc ttgaaggtgc agacgattgg
     2041 ctcgtgacgc agacggtgag cgaggcattg gaggatattc gccccgctgg cctgtgctac
     2101 cttcctacct g
//
LOCUS       DATMXI010000343         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_230647, whole genome shotgun sequence.
ACCESSION   DATMXI010000343 DATMXI010000000
VERSION     DATMXI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230647"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..1172)
                     /locus_tag="VN444_06690"
     CDS             complement(432..1172)
                     /locus_tag="VN444_06690"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWR21529.1"
                     /translation="MTLNIAHRGASALAPENTMAAFEKAVELGADAIELDLHVSRDGE
                     LVVIHDTTLDRTTDGEGPVHARSLQELKRLDAGRWFGESFACQRIPTLAEVLDRFSGT
                     IPLALEIKAGSAFFRGIEEKVVSALREHQVVSRVAVASFDHHALLRLKELEPCLRTGA
                     LLVGRPMSMSAVAGPSKTDAMALECSLVTKTEVDACRAAGLQLVVWVVNEPAQMRHFI
                     DLGVDGIITDSPDLLRQVLSEPRESRRI"
     gene            complement(1226..2224)
                     /locus_tag="VN444_06695"
     CDS             complement(1226..2224)
                     /locus_tag="VN444_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWR21530.1"
                     /translation="MRQSPFQNVPDPTIAPHEVLVKVKACALNHLDLWCRKGMLGITI
                     PLPHICGSDIAGEVAAVGSIVTRITPGQRVVVSPGVSCGQCVHCLSGHDNLCHSYQII
                     GGYRMDGGYAEYVKVQEVNILPMPEGMSFEAAAAFPLTFLTAWNMLVNLAHVKRGDEV
                     LVMGAGSGVGSAAIQIAKLFGARVIAAAGSDEKLDKAKALGADNGINYVSQDLVAEVR
                     RLTAKRGADVIFEHVGGAVFEKLIPVLATGGRLVTCGATAGHLAQTDIRYLFMRQASI
                     MGGFMGPKADLLQIVREIERGTLKPVVDRVFPLKDATEAQRAMEDRKLFGKLVLVV"
BASE COUNT          445 a          726 c          637 g          436 t
ORIGIN      
        1 cttctgggcg gaggcgaaga ggaagtggtg aagcgggata tccggatact tgaggtgcgt
       61 tggaacccat tggtcatagt gggcgaacca gggtcggtca aggccttggt atgtggcagc
      121 aactgtaccc gccattccgc gctccttcaa cttccagaga cctcttctcc cactgacgag
      181 atggccgcgc ctacgacaca acgcccctga atggcaggtg aggccttatg tggaattatt
      241 gtaactactc aggtgtttag gctgtaggct gaaggctgtt agccgttgcg catggaccga
      301 atcaaggcgc ccagaacctt ttcggtctcc atcatgttca cgtcgcattc agaaaacgcc
      361 tggctcatct gtgcgcgttc ctatagtcta aaagcctaca gtctatctac ctgagtagtt
      421 acgatcgctc tttagatcct gcgggactcg cgtggctcgc tcaagacctg acgcaacagg
      481 tctgggctgt cggtgatgat cccgtctacc ccgagatcga taaagtggcg catctgagca
      541 ggctcattta ccacccagac aacaagctga agcccggcgg cgcggcaggc gtcgacctct
      601 gttttcgtga caaggctgca ctcgagggcc atggcatctg tcttgctggg gccggccacg
      661 gccgacatcg acatgggccg tcccacgagt aaagcgccgg ttcgaaggca gggctccagc
      721 tccttcagcc gcagtagcgc atgatgatcg aaggacgcga cggctacccg tgagacgacc
      781 tgatgctcac gcagggccga caccaccttc tcctcaatgc cacgaaagaa ggccgaacct
      841 gccttgatct caagcgcgag cgggatcgtt ccggagaaac gatcgagaac ttcagcgagg
      901 gtcgggatgc gctgacaggc aaagctctcg ccaaaccagc gaccggcatc cagccgcttg
      961 agctcctgca ggctacgcgc atggaccggt ccctcaccat cggtggtccg atcaagcgtg
     1021 gtatcgtgaa tgacgaccag ctcgccatcc cggcttacgt gaaggtccag ctcgatggcg
     1081 tcggctccca actcgaccgc cttttcaaag gctgccatcg tattctctgg cgcaagcgcc
     1141 gacgcacccc gatgcgcgat gttcagcgtc atggtactcc tcctgacctt acgttccacc
     1201 catctactgg tttgtccttt gacgactaca ctaccaggac cagcttaccg aacagcttgc
     1261 ggtcctccat cgcgcgttgc gcctccgtag cgtctttgag cgggaacacg cgatccacta
     1321 ccggcttaag cgttccgcgc tctatctccc gcacgatctg cagcaggtcg gccttcggtc
     1381 ccatgaagcc gcccatgatc gacgcctgcc gcatgaagag gtaccggata tcggtctgag
     1441 ccaggtggcc ggctgtcgcg ccgcaggtca ccaggcggcc cccggtggcc aggaccggaa
     1501 tcagcttctc gaacacagca ccccccacat gctcgaagat cacgtccgcc cctcgcttgg
     1561 ccgtaaggcg acgcacctcg gcgaccagat cctggctgac atagttgatc ccattatccg
     1621 cgccaagcgc cttggcctta tcaagcttct cgtcgctgcc ggcggcggcg atgacgcgag
     1681 cgccgaacag cttagcgatc tggatggcgg cgcttccaac cccgctcccc gctcccatca
     1741 ccagcacctc atcacccctt ttcacgtgcg cgagattcac cagcatgttc caggcggtga
     1801 gaaacgtgag cgggaaggcc gccgcagcct cgaaggacat gccttccggc atcggcagga
     1861 tgttgacctc ctggaccttc acgtactccg cgtagccacc gtccatccgg tagccgccga
     1921 taatttggta ggagtggcag agattgtcgt ggccggacag gcagtggacg cactggccac
     1981 agctcactcc cggcgagacg accacccgtt gaccgggcgt aatgcgggtc acgatgcttc
     2041 ccaccgcggc cacctcgccg gcgatgtcag aaccacagat gtggggcagg gggatcgtga
     2101 tccccagcat cccttttcgg caccagagat ccaggtgatt caaggcgcag gccttcacct
     2161 tcaccagcac ctcatgcggg gcaatggtgg gatcggggac gttctgaaac ggcgactgtc
     2221 tcaggcccca atacgcttcg taca
//
LOCUS       DATMXI010000344         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_50107, whole genome shotgun sequence.
ACCESSION   DATMXI010000344 DATMXI010000000
VERSION     DATMXI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50107"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VN444_06700"
     CDS             complement(<1..358)
                     /locus_tag="VN444_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWR21531.1"
                     /translation="MPLPVVTIVGRPNVGKSTLFNRLVGGRRAIVLDEPGVTRDRLYA
                     TIEWKGCAFMLGDTGGFEPGVKSGLAVQVLAQVQMAVQEAALVVFVVDAREGLTPLDE
                     EIARALRRDVRTRIVVV"
     gene            complement(484..1026)
                     /gene="pgsA"
                     /locus_tag="VN444_06705"
     CDS             complement(484..1026)
                     /gene="pgsA"
                     /locus_tag="VN444_06705"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWR21532.1"
                     /translation="MALALMNLANSLTILRLLMAPVISILLVYKYWRLGLAIFVLAGI
                     TDALDGFIARSRAQGTDLGMILDPLADKLLLFATFMTLVYLQQIPRWLFIIALSRDLI
                     VVGGFLVIYIVKGKATVSVSWIGKITTGLQMLTVLVTLIARVAGGAGLYVSGIIYLTA
                     AVTILSGLDYLRRGTMVLNS"
     gene            complement(1017..2174)
                     /gene="hflX"
                     /locus_tag="VN444_06710"
     CDS             complement(1017..2174)
                     /gene="hflX"
                     /locus_tag="VN444_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWR21533.1"
                     /translation="MRAMIVSAQLSERKERALLVGIHCKRDPRWEAEDSLVELARLAE
                     SAGALVAGTILQERDAPDSRYLIGKGKAQEIKAQWSGKIDLLVVDEELSGSQQRNLEE
                     LTGYRTVDRPLLILDIFAQRARSREGKLQVELAQLDYLLPRLVGRGVEMSRLGGGIGT
                     RGPGETQLETDRRVIRRRMAKIRADLTKVRRHRALLRRPRKRHAIPIVALVGYTNAGK
                     STLFNALTHSGVRTDDALFVTLDPILRRVTTADGFGFLLSDTVGFIRRLPAQLVTAFK
                     ATLEELNEADLLLHVIDASHPQMMEQKQAVDAIVSELGLSAKPVIEVLNKVDLLTSGI
                     APSFVAGKSALLRVACSALTGYGLDRLRQVVQEKLTPASDHAAMEAAVAWP"
BASE COUNT          470 a          750 c          652 g          425 t
ORIGIN      
        1 gtactacgac gatgcgggtg cgtacatcgc gccgaagcgc acgagcgatc tcctcatcca
       61 gtggggtcag cccctcgcgg gcgtcaacga caaagacgac gagggcggcc tcctggaccg
      121 ccatctgaac ctgggcgagt acctgaaccg ccagaccgct tttgacgccc ggttcgaacc
      181 cccccgtatc accgagcatg aacgcgcagc ccttccactc gatcgtcgcg tacagccggt
      241 ccctggttac gccgggctcg tcgagcacaa tcgcccttcg cccgcccacc agccggttga
      301 acagggtcga tttccccaca ttgggccgac caacgatcgt gacgacagga agcggcatcg
      361 cagttctctc caatcggata cgttatcgtg gaataactac tcaggcgttg aggctaaagg
      421 ctgaaggtta tacattaact cgcaaggcac aaatcactcc attaaagacc tttgcggtcc
      481 acttcacgag ttgagcacca tcgtgcctcg ccggaggtag tcgagcccgg acaagatcgt
      541 gaccgctgcc gtcagataga tgatccccga cacgtataac cctgccccgc cagccacacg
      601 ggcaatgagc gtcaccagca cggtcaacat ttgcagacct gtcgtaatct tccctatcca
      661 ggagacagag accgttgcct tgcctttcac gatgtaaatg accaggaaac cgccaaccac
      721 tatcaggtcc cggctgaggg caatgatgaa gagccaccgg gggatctgtt gcaggtagac
      781 cagcgtcatg aaggtggcaa agagcagcag cttgtcggcc agcggatcca ggatcatccc
      841 aagatctgtc ccctgggccc tcgaacgcgc gatgaatccg tccagggcat cggtgatccc
      901 tgccagcaca aaaatcgcca ggccgagtcg ccagtacttg tagaccagca ggatcgagat
      961 gactggggcc atcagaagtc tgaggatcgt gaggctgttg gcaagattca tcagcgctag
     1021 ggccacgcaa cagccgcttc catcgcggca tgatcggacg cgggcgtcag cttctcttgc
     1081 actacctggc gcagccgatc aaggccgtac ccggtcagcg cggaacacgc cacccggagg
     1141 agtgcgctct ttccggccac aaaggacggt gcaatcccgc tcgtcaggag atcgacctta
     1201 ttcaagacct caatgaccgg tttagccgaa aggccgagct cgctcacgat cgcgtctacg
     1261 gcttgcttct gctccatcat ctgaggatga ctggcgtcga tcacatgcag caggagatcg
     1321 gcctcgttga gctcctccag agtggccttg aacgccgtca ccaattgcgc ggggaggcgc
     1381 cggatgaacc ccacggtatc ggagagcaga aagccgaatc cgtcggcagt ggtgacccga
     1441 cgcaggatgg gatcaagggt tacaaagagt gcgtcgtcgg tcctgacgcc cgagtgcgtg
     1501 agggcattaa agagtgtcga cttgccggca ttagtgtagc cgaccagggc tacgatcggg
     1561 atcgcatgac gctttcgagg ccgcctcagc agcgcccgat ggcgacggac cttggtcaga
     1621 tccgcccgga tcttcgccat acgccggcgg atcacgcgac ggtcggtctc gagctgggtt
     1681 tcgccagggc cgcgggtccc gataccgccg ccaaggcgtg acatctcaac ccctcgcccg
     1741 acgaggcgcg gtagcaggta gtcgagctgg gccaactcca cctgaagctt gccctcccgg
     1801 ctcctcgcgc gctgggcgaa gatgtccagg atgagcaagg gccgatcgac ggtcctgtaa
     1861 ccggtcagct cctccaggtt tcgctgctgc gagcccgaca actcttcgtc taccaccagc
     1921 agatcgatct tcccgctcca ctgcgccttg atctcctgcg ccttaccctt cccgatcaga
     1981 tagcgcgagt ccggagcgtc ccgctcttgc aggatcgttc cagcgacaag ggctccagcc
     2041 gactccgcca gtcgggccag ttcgacgagc gagtcctcag cctcccagcg ggggtcgcgc
     2101 ttgcagtgaa tccccaccag cagcgcccgt tcctttctct cgctaagctg tgcgcttacg
     2161 atcatcgccc tcattgccac ttccaaagcc ctctcgacag gccttggccc tcgcaaactt
     2221 tcggtagagc ccttctccgt cttcagcgtt gatcatcact gtcaggtggt caagggcagc
     2281 ctggaattgt ccgatca
//
LOCUS       DATMXI010000345         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_275068, whole genome shotgun sequence.
ACCESSION   DATMXI010000345 DATMXI010000000
VERSION     DATMXI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275068"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>2066
                     /locus_tag="VN444_06715"
     CDS             37..>2066
                     /locus_tag="VN444_06715"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWR21534.1"
                     /translation="MGQRRLSGEPYVQHPIAVAGVLTSLKTDVTAIVAALLHDTLEDT
                     VATPEELEREFGKDVVHLVDGVTKIGKITFRSYEEKQAENFRKMVLSMADDIRVVLIK
                     LADRLHNMRTLQHLRPAKRQEIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPEV
                     YETLRVRVAKRDEDRQQYIDEVRRLVQKAMQENGLVGNVYGRPKHLYGIYQKMHKQSI
                     TFEEVYDLTALRIVTDTKMNCYAVLGVIHSAWRPVPGRFKDYIAIPKSNLYQSLHTTV
                     VGPKGEHVEFQIRTEEMHRIAEHGIAAHWKYKEQGHVADRDSKAFGWLHQFVEWHEDL
                     TDNRQFMDSVKLDLFHDVVYVFTPKGVVKELPRGSTPVDFAFAIHTEVGDHCVGAKVD
                     GKIVPLKHQLASGDTVEILTSPTQSPHKDWLKFVRTSRAKTKIKHWIKLEEQKRSIEI
                     GRRLLESEFRRQGMAPARMFKSAELLEAARQSGFESTDELAAAVGFGRVATSQVIDKL
                     IVSSSKVQAPASSPERLTARKAVSGKSDGTGVQVKGSRDLLMQLSRCCHPVPGDRILG
                     YITRGRGLTIHAVDCPNLEALDYDKERLVEVEWDAATPSTHSVKMSIVAVDKTGVLAN
                     VSSAIAECHANISRAEITTREDQKAVLDFVVEISDTRHLDQVLKAVERVEGV"
BASE COUNT          494 a          532 c          610 g          430 t
ORIGIN      
        1 gttcggaagg cctacgaatt ttctgccaag gcgcacgtgg gtcaacgtcg tctttccgga
       61 gaaccgtatg ttcagcatcc catcgccgtc gccggagtcc tgacctcgct caaaacagat
      121 gtcaccgcca tcgtcgcagc attgctgcac gataccctgg aagatacggt cgcaacgcct
      181 gaagaactcg aaagagagtt cggaaaggat gtggtgcatc tggtcgacgg ggtgacgaag
      241 atcggcaaaa tcacatttcg gagttatgag gaaaagcaag cggagaattt ccgcaagatg
      301 gtgttgtcga tggcggacga cattcgtgtc gttctcatca agctcgccga ccggttgcac
      361 aacatgcgca ccctgcagca tctccgtcca gccaagcggc aggagatcgc ccaggagacg
      421 ctggagatct atgcaccatt ggcaaatcgt cttggaatcg ggtggatcaa gaacgagttg
      481 gaagacctct gtctcaaaca ccttaagcct gaagtgtacg agacattgcg tgtccgcgtc
      541 gcgaaacgcg acgaggaccg gcagcagtat attgacgaag tccgccgact cgtgcagaag
      601 gccatgcaag agaacgggtt ggtcggaaac gtatacggac ggcccaaaca tctctacggc
      661 atttaccaga agatgcataa gcagtcgatt acattcgaag aggtctacga ccttacggcg
      721 ctgcggatcg tcaccgatac gaagatgaac tgttacgccg tgctcggagt gattcactca
      781 gcctggcggc ctgtgccagg gcgcttcaag gactacattg caattcccaa atccaatctc
      841 tatcaatcgc tgcatacgac ggtggtgggc cccaagggag agcatgtcga attccagatc
      901 cgcactgagg agatgcatcg tatcgccgag cacggcatcg ccgcgcactg gaagtataaa
      961 gaacagggcc atgtggccga tcgtgacagc aaagctttcg gctggctgca ccagttcgtt
     1021 gaatggcatg aagatttgac ggataaccgg cagttcatgg actcggtgaa gctcgacctc
     1081 tttcacgatg tggtgtatgt gtttacgccc aagggggttg tgaaggagtt gccgagaggg
     1141 tcgacacccg tcgattttgc attcgcgatt cataccgagg tcggggacca ttgtgtcgga
     1201 gccaaggtgg atggaaagat cgtccccctc aagcatcaac tggccagcgg agatacggta
     1261 gaaatactga cctctcccac gcaatcgccg cacaaggact ggttgaaatt cgtccgcacc
     1321 tcccgtgcga agactaagat caaacattgg atcaaactgg aagagcagaa gcggagcatt
     1381 gagatcggcc gtcgtttact ggagtcggag ttccgccgtc aggggatggc gccggctcgg
     1441 atgttcaaat cggcggagct gctagaggcc gctcgtcagt cgggattcga gagtaccgac
     1501 gaattggctg cggcagttgg gttcggtcgc gtggcgacgt cgcaggtcat cgacaaactc
     1561 atagtttcat catctaaagt gcaggctcca gcgtcctctc ctgaacgtct cactgcgcgt
     1621 aaagcggtca gtggaaaaag cgacggcaca ggtgtacagg tgaaaggatc tcgcgatctt
     1681 ctcatgcagc tctcgcgctg ttgtcatccc gtgcccggcg accgcattct gggatatatt
     1741 acgcgaggtc gcgggctcac aatccatgct gtcgattgcc cgaatcttga ggcgctggac
     1801 tacgacaagg agcggttggt ggaagtggag tgggacgctg ccacacccag cacgcactcg
     1861 gttaaaatgt ccattgtcgc tgtcgataag accggggtct tggcgaatgt gtcctctgcg
     1921 atcgcagaat gccatgccaa catcagtcgt gcggagatca cgacgcggga ggatcaaaaa
     1981 gctgtgctgg attttgtcgt agaaatttca gatacccgcc atctcgatca agtgctcaaa
     2041 gctgttgaac gtgtggaggg ggtaat
//
LOCUS       DATMXI010000346         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_119986, whole genome shotgun sequence.
ACCESSION   DATMXI010000346 DATMXI010000000
VERSION     DATMXI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119986"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="radC"
                     /locus_tag="VN444_06720"
     CDS             <1..665
                     /gene="radC"
                     /locus_tag="VN444_06720"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWR21535.1"
                     /translation="LVGHRERLQARYRLNGEATLEDYELLELLLTYAIPRHDTKLFAK
                     KLLERFGTLASVFEAEPAALEVIDGIGPQAATLISLIRPLATRFVTQAPKAKTILKST
                     VDAAAYFQAKLKGLLEEEVHVAFVNSKNAVMTSECLQRGTVDQSVVYVRKVIEHALTH
                     KASGFLLAHNHPSGDPTPSSDDRDLTQALKAAAATVGVRFLDHLVIGETDPFSFKANG
                     LL"
     gene            715..954
                     /locus_tag="VN444_06725"
     CDS             715..954
                     /locus_tag="VN444_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWR21536.1"
                     /translation="MAKLLITATYGYDNSTRAAMPFFLAKGAKESGIDVGIVLALDAT
                     VLIKPELRQHVKPYGLPPLDELFQFAVDHQIPIYV"
     gene            1190..2158
                     /locus_tag="VN444_06730"
     CDS             1190..2158
                     /locus_tag="VN444_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWR21537.1"
                     /translation="MRAIRMHEYGGPEVLRYEEVALPELGVGEARVKLDAVGVNYIDI
                     YQRKGQYPDPLPVIPGREAAGVVDAVGPGVSDLTPGTRVVYAMQVGAYAEYAVVPVRR
                     LVPIPDALEIRVAAAVMLQGLTAHYLTHSTYPLQPGDRALIHAAAGGVGLLLIQMAKR
                     RGAWVIGTVSTEQKADLARQAGADEVILYTQVDCETETKRLTNGQGVHVVYDSVGQAT
                     FDKSLNCLTPRGYLVLYGQSSGPVPSLNPQVLSTKGSLYLTRPNLMHYTLDRTELLKR
                     ASDLFDWITAGTLTVRIDSTFPLAEAALAHRSLEERKTKGKVLLIP"
     gene            2158..>2801
                     /locus_tag="VN444_06735"
     CDS             2158..>2801
                     /locus_tag="VN444_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWR21538.1"
                     /translation="MRGPGLGLSLLVLLTGSVSCGGANSTQELPLHQIRLPTGFEIGI
                     YASHVPNARSMAISPNGTLFVGTLTAGNVYAIVDRNQDGKADEVITIARGLNMPNGVA
                     FHNDALYVAETDRILRYDNIETRLKDPPAPVVLSTRFPRDRYHGWKFIRFAPDGWLYI
                     PVGAPCNVCEREDERYASIMRMRPDGTQSEIFARGVRNTVGFDWHPKTRELWFT"
BASE COUNT          582 a          815 c          866 g          538 t
ORIGIN      
        1 ctttagtggg gcatcgggag cggctgcagg ctcgctaccg gctcaatggc gaggcgacgc
       61 tcgaagatta cgagctgctg gaactcctcc tgacgtatgc cattcccagg cacgacacca
      121 agctattcgc caagaagctc ctggaacgat tcgggacgct agccagtgtc tttgaggctg
      181 agccggcagc cctggaagta atagacggga tcgggcccca ggcggctacg ctgatttcac
      241 tgattcgacc cctcgcgacg cgctttgtca cacaggcccc aaaggcgaaa acgatactca
      301 agtccaccgt ggatgccgcc gcctattttc aggccaagct gaaggggtta ctcgaagaag
      361 aggtccatgt cgcgttcgtg aattcgaaga acgcggtgat gaccagcgag tgtctgcagc
      421 gcgggacggt cgatcagtca gttgtgtacg tcaggaaggt gatcgagcac gccctgacgc
      481 ataaggctag cggcttcctc ctggcccata atcacccgag cggtgacccg acgccatcta
      541 gcgatgacag agatctgacc caggcactga aggccgcggc ggcgaccgtt ggtgtgcgct
      601 tcctcgacca cctggtcatc ggcgagactg atccctttag cttcaaagcc aacgggctgc
      661 tgtagcggat ggaacggaaa ggtagagttg aacacacaga gaatggaggg gacgatggcg
      721 aagttgttga ttacagcgac ctacgggtac gacaactcca cgcgggcggc gatgccgttc
      781 tttctggcga agggggcgaa ggaatcgggg atcgatgttg gcatcgtcct tgcgcttgat
      841 gccacggtcc tgatcaaacc ggagctacgg cagcacgtca agccgtacgg acttccgcca
      901 ctcgacgagc tattccagtt cgccgtggac catcagatcc caatctacgt ctgagggggg
      961 tgagcccagg ctcgaggggt cttggacgaa gacctcaagc gtgtgtcagt cgtcgagaaa
     1021 atcgacatca aacggtacgc ccagctactc atggagtacg accggtcggt tacgtactga
     1081 gcactcagcg atcagcattc agctttcagc gatgactcag aacgattgtt cttaaagctg
     1141 agtgctgaca gctatctcgc tgaccgcttt tccagaggga gacaacagta tgcgcgcgat
     1201 tcgtatgcat gagtatggcg gtcctgaagt gcttcgctac gaggaggtgg ctctccccga
     1261 gctcggcgtg ggcgaagcgc gggtcaagct tgacgcggtg ggggtcaact atattgacat
     1321 ctaccaacgg aaaggccaat accctgaccc gttgccggtg atccccggcc gggaggcggc
     1381 cggcgtagtg gatgcggtcg gcccgggcgt gtcggacctt acgccgggga cccgcgtcgt
     1441 ctacgccatg caggtgggtg cctacgcgga atatgcggtg gtcccggtgc ggaggctggt
     1501 accaattccg gacgcattgg agatacgagt agccgcggcg gtgatgctgc aggggctgac
     1561 cgcgcactat ctgactcaca gcacctatcc actgcagccg ggcgaccgcg cactgattca
     1621 tgcagccgcg ggtggcgtcg gcctgctcct gatccagatg gccaagcggc ggggagcctg
     1681 ggtcatcggg accgtctcga cagagcagaa ggcagatctg gcgagacagg ccggtgctga
     1741 tgaggtgatc ctctacacac aggttgactg tgaaacggaa accaagcgct taaccaatgg
     1801 ccaaggcgta cacgtggtct acgactcggt cgggcaggcc accttcgaca agagcctgaa
     1861 ctgtctcacg cctcgggggt acctcgtgct ctacggccag tccagcgggc cagtgccatc
     1921 gctgaacccc caggtgctca gcaccaaggg ttcactgtat ctgacccgtc ctaacctgat
     1981 gcactataca ctcgaccgga ccgaactact gaagcgggca tcggatctgt tcgactggat
     2041 cactgccggt acgctcaccg tgcgaatcga ctcgaccttt ccgcttgccg aggccgccct
     2101 cgcccatcgc tcccttgaag agcgcaagac caaaggaaag gtcttgctga tcccctgatg
     2161 cgcggccctg gactcggtct gtccttgctc gtgttgctga caggatcggt gtcttgtggc
     2221 ggagcaaaca gtacgcagga gttgccgctc catcagatca gactgccgac agggtttgag
     2281 atcggcatct acgcgagcca tgtccccaac gcccgttcaa tggccatcag cccaaacggc
     2341 acgctatttg ttggcaccct cacggcaggg aatgtctatg ctatcgtcga tcgcaaccag
     2401 gacggcaagg cggacgaggt cattacgatc gcgcgagggc tcaatatgcc gaatggcgtc
     2461 gcattccaca atgacgcgct ctacgtggcc gagacggacc ggatcctgcg gtacgacaat
     2521 atcgaaactc gcctgaaaga cccgccagcg cctgtggtgc ttagtacacg cttcccccgg
     2581 gatcgctatc acggctggaa gttcatccgc tttgcccccg atgggtggct gtacatcccc
     2641 gtaggcgccc cgtgtaatgt ctgtgagcgc gaagatgagc gctatgcctc gatcatgagg
     2701 atgaggccgg atgggaccca atcagagatc ttcgcgcggg gcgtccgcaa tacggttggg
     2761 ttcgattggc atccgaagac gcgtgagctc tggtttacgg a
//
LOCUS       DATMXI010000347         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_120093, whole genome shotgun sequence.
ACCESSION   DATMXI010000347 DATMXI010000000
VERSION     DATMXI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120093"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..866)
                     /locus_tag="VN444_06740"
     CDS             complement(108..866)
                     /locus_tag="VN444_06740"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HWR21539.1"
                     /translation="MSTTIIPTTSTTFRRPRRGARHRILPALVGLTVILLGLVSMTSA
                     AKHTPNPGSIALPISGTMDEKGVPVGWDLETFQNHHQIKIEPLKDGQFGIRLVSEESS
                     FGLHKVIEVDLKEFPILSWRWKVDRLPLAGDIREKNKNDQAAQLYVIFPNSLIRLRSP
                     MLGYLWDSNAPAGTTADGYSPVTPIKVIVLRSGKQQLGKWVQERRNVAEDYVRLFGQN
                     SLPKVGRVAIWINSQHTKSTAQSSFADLQFQRAN"
     gene            1417..2187
                     /locus_tag="VN444_06745"
     CDS             1417..2187
                     /locus_tag="VN444_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HWR21540.1"
                     /translation="MFNPLEAVGRTVLKQVQAMGRMAIFLGSTGFWAVRPPLKFRRIV
                     GQVYFIGVQSSSIILLTAAFSGMVLGLQGYYTLRKFGSEALLGPAVGLSLIKELGPVM
                     AALMVTARAGSASAAEIGIMRITEQIDALEAMAVNPMKYLVVPKVIAGLIAVPLLTGI
                     FDVVGIYGGYLVGVKLLDVSAGTYFSEMRNMVEMSDIRGGFLKSLGFGLIITWVCTYK
                     GFYAGYGAEGVGKATTEAVVLSSVLILVWNYFMTAILF"
     gene            2193..>2518
                     /locus_tag="VN444_06750"
     CDS             2193..>2518
                     /locus_tag="VN444_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21541.1"
                     /translation="MIQIIDLYKSFEQQQVLNGVNLTIPRGQVTAIIGRSGGGKSVLL
                     KHLIGLMQPDSGQVLVDGIDLGRLRGKALDLVREKFGVLFQGGALFDSMTVFDNVAFP
                     LREKTRL"
BASE COUNT          531 a          640 c          756 g          591 t
ORIGIN      
        1 ggaacgggcg cactaacgga ggctcgggga atgactgaga agcgcattcc tctgacgcaa
       61 acccgcggtc gagatggtaa cgcgtcgtag ggtagcgcgg ttactgccta attggctcgt
      121 tggaactgaa gatcggcgaa cgacgactgg gcagttgact tcgtgtgctg ggaattgatc
      181 cagattgcga cacgccccac cttggggagt gagttctggc cgaaaagtcg cacgtagtcc
      241 tcggcgacat ttcggcgctc ctggacccat ttgcccaact gctgttttcc actccgcagg
      301 acaataacct tgataggggt gacgggggaa tagccgtcgg cagtagttcc tgcgggggca
      361 ttactgtccc agaggtagcc gagcatgggg cttcgcagtc tgatcagcga gttcgggaag
      421 ataacgtaga gctgggcggc ctgatcgttt ttgttctttt cacggatgtc acctgcgaga
      481 ggaaggcgat ctaccttcca ccgccagctc agtatcggaa actctttcag gtccacctct
      541 atcaccttgt gtagcccaaa agagctctct tcactcacga ggcggatgcc gaattgacca
      601 tccttaagag gctcgatctt gatctgatga tggttctgaa aggtttccag gtcccagccg
      661 accgggactc ccttctcgtc catcgtcccg ctgatgggca gggcaatact ccctgggttg
      721 ggggtgtgct ttgctgcaga cgtcatcgat acaagcccta acagtatcac tgtaagaccg
      781 acgagcgcag gcaagatccg atgacgcgcc ccacgtcttg ggcgtctgaa tgttgttgag
      841 gttgtcggga tgattgtcgt gctcaactca gcctcctcgc ctgggcatca catccaggca
      901 tcggtgatgc tgcatcagga ctaacggaca atcggcggtc gaccttcagg tattcctggt
      961 gggttgaccg cgacgcactg atggctgatg cgtatcttca taaggaatga gtagtataat
     1021 aatttagtca tgatctcggc acaagggttt tcttcaggaa gcgtgtaacg gatggtaact
     1081 ctaaatactg acggcagggt tcaggggcgc ttaggctcag gatcaggaac agaccaaaac
     1141 ctcgatcaag gaaaggtaac cattcagtta cgggtggaaa atctccccta acccctcttt
     1201 tccaaagagg gggactaacc gtttccccct ttgaaaaagg gggattaagg gggattctat
     1261 aaaaaatacc acattttatt taatgatttt cccgcgcatc actgaatggt tacagggaaa
     1321 gataggaaac aattgacaac ttgggggagc gtatgtaatc ctaccagcct gtcagttgct
     1381 tcgccggcgt actgagaatc tctcttggag tagggaatgt ttaacccgtt ggaggccgtc
     1441 ggacgcacgg ttctcaagca agtccaggcc atggggcgta tggccatctt cttgggttcc
     1501 accggtttct gggcggtccg gccaccactg aagttcaggc gtattgtcgg tcaggtctat
     1561 tttatcgggg tgcagtcaag ctctattatt cttctcaccg cggccttcag cggaatggtc
     1621 ctggggcttc aggggtacta cacgcttcgg aagttcgggt ccgaggccct tctcggtccg
     1681 gccgttggcc tgagcctgat caaggagctg gggccggtga tggcagccct gatggtgacg
     1741 gcgcgggccg gatcggcctc tgccgcggag atcggcatca tgcggatcac cgagcagatc
     1801 gacgcgttgg aggcgatggc cgtcaatccc atgaaatacc tggtagttcc caaggtgatc
     1861 gcaggactga ttgctgtccc gctcctgacc ggtatcttcg atgtggttgg gatctatggc
     1921 ggctaccttg ttggggtgaa gctgctcgac gtcagcgccg gcacctactt ttccgagatg
     1981 cggaacatgg tagagatgag tgacatacgg ggcggcttcc tgaagtcgct gggcttcggc
     2041 ctcattatca cctgggtgtg cacgtacaag gggttttacg ccgggtatgg ggccgagggc
     2101 gtgggaaaag cgacaacaga ggccgtggtc ctctcgtcgg ttctgatact ggtctggaac
     2161 tacttcatga ccgccatcct gttttaaact ggatgatcca gatcatcgat ctttacaaaa
     2221 gctttgagca gcaacaagta ctcaatgggg tgaacctgac cattccacgg gggcaggtta
     2281 cggccattat tgggcggagt ggaggcggta aaagcgtgct gctcaagcac cttatcggcc
     2341 tcatgcaacc ggacagcggt caggtgctgg tcgatgggat cgacctcgga cggctccgcg
     2401 gtaaggccct ggatctggtg cgcgaaaagt ttggcgtcct gtttcaggga ggcgccctgt
     2461 ttgactcgat gacagtgttc gacaatgttg ccttcccgct gcgagaaaag acccgact
//
LOCUS       DATMXI010000348         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_96552, whole genome shotgun sequence.
ACCESSION   DATMXI010000348 DATMXI010000000
VERSION     DATMXI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96552"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VN444_06755"
     CDS             complement(<1..1383)
                     /locus_tag="VN444_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWR21542.1"
                     /translation="MESITQEPPVTPAPVQSEPIPEGSSASIPKVNGILEWFTHWSIE
                     RRVLAGFGLVFAGIVVISAISYRNTSVLIENSRLDTRSHELVQLLGSVREALDAAESG
                     HRRYLVTGDESYLRPYRNLAEQMPEYLRYLRGLTSEDAEQQTRVETLERLIAKQLGAE
                     AEAITQREKHGYQAVRHLALSGAAKAELEGIQRLMTELDTAEQRSLVQRVAQSAGSTR
                     NTIALLGLGALMQLLLLAFVYFLIHHDVTERRRVAKELQTRGELLQAANKELEAFSYS
                     VSHDLRAPLRHIDGYAALLSKSAGDTLGDKPRRYLQTISDSAKQMGQLIDDLLVFSRM
                     GRQDMLRTTVNLDQLVTTVLHDLRLDLQGRTISWTIQPLPPVLGDPAMLRQVFMNLIS
                     NAVKFTATRTNAEITIGATNRSLSEAEIFVRDNGVGFDMQYVNKLFGVFQRLHRNDEF
                     EGTGIGLANVR"
     gene            complement(1512..>2435)
                     /locus_tag="VN444_06760"
     CDS             complement(1512..>2435)
                     /locus_tag="VN444_06760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWR21543.1"
                     /translation="FSDMTTAQATPGLAIGFLLIFAALGFKIGAVPFHSWIPDTYHGS
                     PTPVTAFLSIAPKGAAFAILLRMFFVALATFKPVWILLIVAASILSMTYGNIVAIAQK
                     NMKRLLAYSGIAQIGNVLIGLATGTKMGSDALLFYLLTYLFANLGAFAVVIAVSEAIG
                     SDEIDDYSGLGRRSPFLAFSMLIFLLSLAGVPPLAGFIGKLYIFVSAVKEGLYLLITV
                     GLINVVISMYYYLIVVKKMYVSEPHDRAPLRVSGSIKLVVYVCLAGTLAIGIYPQPVT
                     DWVVSALLMFSNFPDPTAALPVPPAVTPFGG"
BASE COUNT          563 a          653 c          720 g          499 t
ORIGIN      
        1 acgaacgttc gcgagaccga tgccggtccc ttcaaactca tcgttacgat ggagtcgctg
       61 gaagacgccg aacaatttgt tgacatattg catgtcgaac cctactccat tatcccgtac
      121 gaatatttca gcttcgctga ggcttctatt ggtggcgccg attgtgattt cggcgttcgt
      181 tctggtcgcc gtgaacttta ccgcgttgga gatcaaattc atgaacactt ggcgcaacat
      241 cgcaggatcg cctaatacag gaggtagtgg ctgtattgtc caggagattg tccgcccttg
      301 caagtcaaga cgcaaatcat gaaggacggt ggtgacaagt tggtccaagt tgacggtcgt
      361 gcggagcata tcctgacggc ccatgcgaga aaacacgagc aggtcgtcga tcagctgacc
      421 catctgcttc gcggaatccg aaatcgtctg cagataacgg cgcggtttgt cgcccagcgt
      481 gtcaccagcc gacttggaca acagagccgc atagccgtcg atatggcgga gtggggcgcg
      541 caggtcgtgc gacacggaat agctgaatgc ttccaactcc ttattggccg cctggagcag
      601 ctcgcctctg gtttgtagtt ccttggccac gcggcggcgc tcggtgacat cgtgatggat
      661 cagaaagtat acgaaggcca agaggagcaa ctgcatgagg gcgccgagcc cgagcaaggc
      721 gatcgtattt ctcgtgctcc cagcggattg cgccactcgc tgcacgagcg atcgttgttc
      781 cgcggtgtcg agctccgtca tcaggcgttg gattccctcg agctcggcct ttgccgctcc
      841 cgagagcgcg agatggcgga cggcttggta gccgtgtttt tcacgttgag tgatggcttc
      901 cgcctccgct cccaattgct tggcgataag ccgctcgagc gtctccacgc gggtctgttg
      961 ttcagcatcc tcgctcgtca ggcctctgag ataccggagg tattcaggca tctgttcagc
     1021 caggttccgg tatggtctga ggtatgactc atcaccggta accaggtatc gtcgatgacc
     1081 actttcagcg gcatcgaggg cctctctcac tgaaccgagt aactggacca gttcgtggct
     1141 cctggtatcg agccggctgt tctcgatcag aacggaggtg ttgcgatagg aaatcgcact
     1201 gatgacgaca atcccagcaa aaacgagccc aaacccggca agaactcgtc gttcgatcga
     1261 ccaatgggta aaccactcca agatcccgtt gaccttggga atcgaggctg aactaccctc
     1321 ggggatcggt tccgattgaa ctggagcggg cgtaactgga ggttcctgcg tgatggattc
     1381 catatcagtc aaacgtgctg ctgcaccgga atggaggcca ctcttgtgga gactgtaccc
     1441 tcctccagaa gaccctgcaa gaaatatcct acaggatact ggctgcaccg tggaggcagt
     1501 ccacagtaga actacccccc gaagggggtg acggcagggg gaactgggag ggctgcggtt
     1561 ggatcaggga agtttgaaaa cattaagaga gcagaaacaa cccagtcggt gaccggttgc
     1621 ggatagatgc caatggcaag tgtgccggcg agacagacat agaccaccag cttaatcgag
     1681 cctgagaccc tgaggggcgc gcggtcatgg ggctcgctga catacatctt cttcacgacg
     1741 atgaggtagt aatacatgga gatcacgacg ttgatgagcc ctaccgtaat gagcaagtac
     1801 agcccttcct tcacggctga gacaaagatg tacagtttgc cgataaagcc ggccagcggg
     1861 ggcacgccgg ctagcgacaa gaggaaaatc aacatggaaa atgcgagaaa tggcgaccgg
     1921 cggcccagac cgctatagtc gtcgatttca tcactgccga tggcttcgct gaccgcaatg
     1981 acaacggcaa aggcaccgag gttggcgaac agatacgtca ggaggtagaa cagcagtgcg
     2041 tcgctgccca ttttcgtccc cgtcgcgaga ccaatcagta cattgccgat ctgggcgata
     2101 ccggaatagg ccaggagccg cttcatgttc ttctgcgcga tcgccacgat gttgccgtat
     2161 gtcatggaca ggatggacgc agcgacaatc aacagaatcc agaccggctt aaaggtggcc
     2221 aaggcaacga agaacattct cagcagaatg gcaaaggccg ctcccttcgg tgcgatcgag
     2281 aggaacgcgg tcaccggcgt cggtgatcca tggtatgtgt cgggaatcca cgagtggaag
     2341 ggtaccgcgc cgatcttgaa ccctaatgcg gcgaagatga gcaggaaacc aatggccagt
     2401 cccggtgtgg cctgcgccgt tgtcatatcg gaaaa
//
LOCUS       DATMXI010000349         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_316534, whole genome shotgun sequence.
ACCESSION   DATMXI010000349 DATMXI010000000
VERSION     DATMXI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316534"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1306)
                     /gene="ftsA"
                     /locus_tag="VN444_06765"
     CDS             complement(71..1306)
                     /gene="ftsA"
                     /locus_tag="VN444_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWR21544.1"
                     /translation="MTRRGELVTGLDIGTTKICVIVAEVTENGVEIVGCGISPSQGLK
                     KGIVVNIDVTVESIKQAVEAAEAMAGVALDSAFVGIAGGHIKGINSRGVIAISGKNQE
                     ITQNDVDRVIEAAKAITLPADRRVIHVIPQEFIIDDQGGVKEPVGMSGSRLEAEIHIV
                     TGAVASAENIIKCATRSGLEVRDIVLQPLASSEATLTADEKELGVILIDIGGGTTDIA
                     VFVDGSIRHTAVLPLGGDHLTHDIAIGLRTPPKCAEEIKHKYGCALASLVEGEESVEV
                     PSVGGRKPRLLSRQMLCEIVQPRMDEIFTHAALEVKRTGLMQQIGAGVVVTGGSSAMA
                     GVPELAEQIFDLPVRLGIPVGIGGLKEVVSSPMHATGVGLVLYGAAHLNQHRFSRPSE
                     QSLVDKIIKRMRQWFSDFL"
     gene            complement(1303..>2182)
                     /locus_tag="VN444_06770"
     CDS             complement(1303..>2182)
                     /locus_tag="VN444_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWR21545.1"
                     /translation="DHPSQVEEGVGFRPNRRFLRLVSARSGWRRKGGIRMGQRFGSRL
                     RQGAMVAILSILLVVLGWLVWQQVSRSTPIHYFLISDLIVEGNRRVPTAAIIDSLALP
                     AHAGILQVDLKEVAAAILRNPWIKTARVSRRLPATLLVHVSERTPRAVVAVDRSYLVS
                     EDGLILQEASASEMSDLPLLKLPADHPLETGELIDPARVEQGTYLWQWFHQGTLGPDV
                     QVKEIQLEGDGSYTVLLDHGLPYLRFGEKDGLQQQLDRLSRALEMRGATLRELEYADL
                     RFADKVIVKPLPKEGA"
BASE COUNT          442 a          720 c          564 g          456 t
ORIGIN      
        1 atgttggatg ctagatcgct agatggtgca tcttgtccca aacccaaggt ctacgctctt
       61 cccgtaagct tcacagaaaa tcgctaaacc actgtctcat ccgcttgatg atcttgtcca
      121 ccaggctttg ttccgatggc ctgctaaacc gatgctggtt aagatgtgcc gcgccgtaca
      181 gcaccaggcc cacgccggtt gcatgcatcg gtgagcttac cacttccttc aggccgccga
      241 tgccgaccgg tatccccagc cgtaccggca ggtcgaagat ctgttcggcc agctccggca
      301 cacccgccat cgcggaggat ccaccagtga ccacaacccc ggcgcctatc tgttgcatca
      361 acccggttcg tttcacttcc agggctgcat gcgtgaagat ctcatccatt cttggctgaa
      421 cgatctcgca caacatctgc cgcgagagca ggcgaggctt acgtccgcca acgctcggga
      481 cctccaccga ctcctcaccc tcaacgagag aggccagagc gcagccgtac ttgtgcttga
      541 tctcctcggc acacttggga ggtgtcctga gtccaatggc aatgtcatgg gttagatggt
      601 cgccgcccag cggcagcacc gcagtatggc ggatgctccc atccacgaag acagcgatat
      661 cagtagtccc gccgccgatg tctataagga ttactcccag ctctttctca tctgcggtca
      721 gcgtcgcttc actggaggcc agcggctgca atacgatatc ccgcacctcc agcccggacc
      781 tggtcgcgca cttgatgata ttttctgccg aggcgacggc acctgtgacg atatggatct
      841 cagcctcgag ccgagatccg ctcattccga ccggttcctt caccccaccc tgatcgtcga
      901 taatgaactc ttgtggaatg acgtggatca ctcggcgatc ggcaggcagt gtgatggcct
      961 tggccgcctc gatgacccga tccacatcgt tctgagtgat ctcctggttc ttaccggaga
     1021 tagcgatgac cccccggcta ttaatcccct tgatgtggcc tccggcgatc cccacaaatg
     1081 cggaatccag agcgactcct gccatcgcct ccgcggcctc gactgcctgc ttaatcgact
     1141 caacggtcac atcgatgttg acgacgatgc ccttcttcag gccctgcgat gggctgatcc
     1201 cgcatccgac gatttcgacc ccgttctccg tcacttcagc gacgatgacg cagatcttgg
     1261 tcgtgccgat gtcgagtcct gtcacgagct cgcccctccg ggtcatgcgc cttccttcgg
     1321 aagcggcttc acgataacct tatcggcaaa ccggagatcg gcatattcca gctcgcgcag
     1381 ggtggcccca cgcatctcaa gcgcccgcga caaccgatcc aactgctgct gcaggccatc
     1441 cttctctccg aaacgcaggt atggtaaccc gtggtccagt aagacggtgt agctcccatc
     1501 gccctccagt tggatctctt tcacctgcac atccggtcct aaggtgccct gatgaaacca
     1561 ctgccacaga taagtcccct gttcgacgcg agcgggatcg atcagctccc ccgtctcgag
     1621 tgggtgatcg gcaggaagct tcagcagcgg aaggtcggac atctccgatg ctgaagcttc
     1681 ctgcaggatg agcccatcct cactgacgag gtaggagcga tcgaccgcca caaccgcgcg
     1741 tggcgtgcgc tccgacacgt gaaccagtaa ggttgctgga agacgtctgc tcaccctggc
     1801 cgttttgatc cagggattgc gtagaatggc cgccgccacc tccttcaaat cgacctgcag
     1861 gatgcccgca tgggctggca gggccaagct atcgataatc gccgctgtag gcactcgtcg
     1921 gtttccttca acaatgagat cgctaatcag aaaatagtgt atgggcgtgg agcgtgaaac
     1981 ctgctgccaa accagccatc caagtacgac cagcaatatg gagaggatgg ccaccatcgc
     2041 accctgtctg aggcgcgaac caaaccgctg gcccatgcga atcccgccct tccgccgcca
     2101 tcctgacctc gccgatacca atctcaggaa gcgccgattt gggcgaaatc caacaccctc
     2161 ctcaacttgc gatgggtgat cc
//
LOCUS       DATMXI010000350         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_316627, whole genome shotgun sequence.
ACCESSION   DATMXI010000350 DATMXI010000000
VERSION     DATMXI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316627"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VN444_06775"
     CDS             <1..379
                     /locus_tag="VN444_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21546.1"
                     /translation="RLESLLQELDLTHLAKSKAYTLSGGERRRAEITRSLVTSPDFML
                     LDEPFAGIDPIAIADIQAIIARLKVKGVGVLITDHNVRETLKIVDRAYLIHEGKVLVS
                     GTANELASDERAREIYLGERFSL"
     gene            391..1815
                     /gene="rpoN"
                     /locus_tag="VN444_06780"
     CDS             391..1815
                     /gene="rpoN"
                     /locus_tag="VN444_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWR21547.1"
                     /translation="MAFEAKLSLRQMQKMVMTPMLQQAINLLQLSRMELLQAVRQEVE
                     ENPVLEEMIEETEELDDLSALNPVAEELTAEHNGEGQAAEVDWESYLQDASDYRPSIQ
                     YESVDRFERESLLTKRGSLQDHLLFQLHLTIRDQDLVRLGGIIIGELDDNGYLRSSIE
                     GLASLAGVSLEPMESALRLIQGFDPAGVGARDLQECLLIQLKMRPEEHAVAEAIVRSH
                     LHDVERHRFGKITAALGVSLRDVQGAVALIASLEPKPGKNYSSEEPQYITPDVYILKL
                     DGQLVVTLNEDGLPRLRVCRYYRQILSKQAPTSREARGYVEEKMRSALWFIRSIEQRK
                     RTLIKVAESLVKYQRDFFEHGISHLKPLTLREVADDISMHESTVSRVTTSKYVQTPQG
                     LFGLKYFFHCGVPSIVGEAVSSRRVKDMVHRYLTKEDSRKPLSDQKIVEILARVHGVE
                     IARRTVAKYRGQLKIPSSNQRRYV"
     gene            1858..2400
                     /gene="raiA"
                     /locus_tag="VN444_06785"
     CDS             1858..2400
                     /gene="raiA"
                     /locus_tag="VN444_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWR21548.1"
                     /translation="MQITITTRNIENTEALKRYAEEKIARLQKFVDHITSVHIVLSGE
                     KHRQIAEVTLHVREHTIRGEESSADLYSAIDLVADKIERQILRYKEKVVDHSGRGAGR
                     SRSKEELVSTEAESFLEEGPSIVKTKSFAIKPLHPDEAAVHMDLLSHNFFVFRNAQTQ
                     EVNVLYRRRDGDYGLIEPTN"
BASE COUNT          628 a          660 c          767 g          571 t
ORIGIN      
        1 acggcttgag agcctccttc aggagttgga tctcacccac cttgccaaga gcaaggccta
       61 cacgctatca ggtggcgaac gtcgccgggc ggagatcact cgatccctgg taacctcccc
      121 ggatttcatg ctgctggacg agccgtttgc cggaatcgat cctattgcga tagccgatat
      181 ccaggccatc attgccaggc tcaaagtgaa gggagttggg gtcttgatca ccgatcacaa
      241 tgtacgtgag accttgaaga tcgttgatcg cgcgtatctg atccacgaag gtaaggtatt
      301 ggtctccggc accgccaacg agctggcatc agacgagcgg gccagggaga tctacctggg
      361 cgaacgattt agtctgtaag gatgaacgct atggcgtttg aagcaaagct cagtctgcga
      421 cagatgcaga agatggtcat gacgccgatg ctgcagcagg cgatcaacct cttgcagttg
      481 tcgcggatgg agttgcttca ggcggtgcgg caggaggtgg aggagaatcc ggttctggaa
      541 gagatgatag aggaaacgga ggaactggac gatctatcag cgttgaatcc cgttgctgag
      601 gagctaacgg ccgagcacaa cggggaaggg caagccgcag aagtcgactg ggaaagttat
      661 cttcaggacg cctcagacta tcggccatct atccaatacg agtcggtcga tcgcttcgag
      721 cgcgagagct tgctcaccaa gcggggttcc cttcaggacc atctgctctt tcagcttcac
      781 ctgaccatca gggatcagga tcttgtcagg ctgggcggca ttatcattgg agagctggac
      841 gacaacggat accttcgcag cagcatcgag gggctggcgt cgctggctgg cgtttcgttg
      901 gagccgatgg aatcggccct tcgacttatc cagggcttcg atcctgccgg ggtgggcgcg
      961 cgcgatcttc aggaatgcct actcatacag ctcaagatgc gaccggagga gcatgcggtg
     1021 gcagaggcca ttgtcagaag ccacctccat gacgtggagc gtcaccgatt tgggaaaatt
     1081 acggctgcgc ttggggtctc gctcagagat gtgcaagggg cggtggcgct tatcgcctcc
     1141 ctggaaccta aaccgggaaa gaactacagc agtgaggagc cgcaatacat tacgcccgac
     1201 gtgtatattc tcaagcttga tggacaattg gtagtaacgc tcaatgaaga cggtcttcca
     1261 cgccttcgag tctgtcgata ttaccggcag atcttatcca agcaagcgcc cacttcacgt
     1321 gaagctcggg gttacgtcga agagaagatg cgctccgccc tatggtttat ccggagcatt
     1381 gaacagcgga aacggacgct tataaaagtg gccgaaagtc tggtgaaata tcagagggat
     1441 ttcttcgagc acgggatctc tcacctcaag cccctgacgc ttcgcgaagt ggccgatgac
     1501 atctcgatgc atgagtccac cgtcagtcga gtgacgacga gtaagtatgt tcagacgccg
     1561 cagggactgt tcgggctgaa atactttttc cactgtggtg ttccatcaat cgttggtgaa
     1621 gccgtctctt ctcgcagggt gaaggacatg gtccaccgat acttaacgaa agaagactct
     1681 cgcaagccgc tgagcgacca gaagatcgta gagatcctgg cgagagttca cggggtcgag
     1741 atcgcccgtc gaactgtggc caagtatcgt ggtcagctta agatcccctc atccaatcaa
     1801 cgtcgctatg tgtaatttgg tacagaggca tttcatcctt tgtcgaggag tcaagtgatg
     1861 caaattacca ttactactcg caacatcgag aacaccgaag ccctcaaacg gtatgcggag
     1921 gagaagatcg ctcggctaca gaagttcgtg gatcatatca cctcagtcca tatcgtcctc
     1981 tcaggcgaga agcatcgaca gatcgccgag gtgacgctcc acgtgcgaga gcatactatc
     2041 aggggagaag aatcgagtgc tgacctgtat tctgccatcg accttgtggc cgacaagatt
     2101 gaacgccaga tccttcgcta caaggagaag gttgtggatc actccggtcg aggcgcgggc
     2161 cgatcacgtt ccaaagaaga gctggtgtct acggaggccg agtcctttct ggaggagggt
     2221 cccagcatcg ttaagaccaa gagtttcgct ataaaaccgc tccatcctga tgaggctgct
     2281 gtccatatgg acctgttgag ccacaatttt ttcgtattcc gaaatgccca gacgcaggag
     2341 gtcaatgtcc tgtatcgaag acgtgatggg gattacggct tgatcgaacc cacgaattga
     2401 gatccctgac ctgtaatcgt aaggcgtaag gcgaagcact gttcattccg tgcgcctcac
     2461 gacggttctt gctgaaaact gatagctgaa tgctgatctt taaacatgct gacgctgctc
     2521 tgaggattga gtggcgggga agtcgtcgca tagcgaacgt gaagggcgca atgtggtgaa
     2581 agcaacggag cttgtgatta tcaccggtat gtctggagcc ggtaag
//
LOCUS       DATMXI010000351         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_31906, whole genome shotgun sequence.
ACCESSION   DATMXI010000351 DATMXI010000000
VERSION     DATMXI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31906"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VN444_06790"
     CDS             complement(<1..417)
                     /locus_tag="VN444_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWR21549.1"
                     /translation="MSKIVQKLDDYRWLLPQDYKPGMRVPGIIYADEALMEAIMKDLS
                     LEQVANGAFLPGIVKASFAMPDIHQGYGLPVGGVVATDITDGVVSPGAVGYDINCGVR
                     LLRTELTKEEVRPRLKELVLALFHEIPTGVGSRGRIR"
     gene            complement(437..1510)
                     /gene="mqnC"
                     /locus_tag="VN444_06795"
     CDS             complement(437..1510)
                     /gene="mqnC"
                     /locus_tag="VN444_06795"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HWR21550.1"
                     /translation="MAESNDRIEAIAEKVEVGERLTFDEGVELFERATLLDLSAMADR
                     VRWRLHPEPIVTYVIGRTVNYTNICWVQCSFCAFYRLPTSPEAYLLSKEEIFQKIEEL
                     IELGGTEVLLQGGLNPSLKIDYYEDLLASIKARYPVHLHALSTVEISYISDSSKISIK
                     DTLKRLKAAGLDSIPGAGAELLVDEVRERISPLKESSSDWLNLMRIAHGLGIPSTATM
                     MYGVGETSAQRVEHLIQIRELQDQTAGFTAFIPWSYQPNGDALGGTGGSGYGYLRTVA
                     VSRIMLDNVKHIQAAWLTMGPKIGQLSLQYGVNDFGSTVLEENVVRTAVTKQLMSLEE
                     IRRNIRDAGLVPKRRNTRYQLLE"
     gene            complement(1543..1986)
                     /locus_tag="VN444_06800"
     CDS             complement(1543..1986)
                     /locus_tag="VN444_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HWR21551.1"
                     /translation="MRVFLDTNVLASAAATRGLCADVLREVLTSHELLTSVQVLSELR
                     RVLRTKFGVAQDLIADFIWLMRQDTVLAQPGQLPTVDIEDQDDLLILSAAISAGADVF
                     VTGDQELLDIGHVENLAILSPRQFWEKLKTQLQRGAGRGKSHRVP"
     gene            complement(1983..2204)
                     /locus_tag="VN444_06805"
     CDS             complement(1983..2204)
                     /locus_tag="VN444_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWR21552.1"
                     /translation="MKTSILTIRLDKDLDDLLTKASRRSGRNRSEVAREALRRQLRIS
                     QFEALRRKIMPFAEARGYLTDEDVFAEVS"
     gene            2375..2656
                     /locus_tag="VN444_06810"
     CDS             2375..2656
                     /locus_tag="VN444_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR21553.1"
                     /translation="MAILKFRHKGLERFFLRGTTVGIQSKHARRLRLILGRLNVAREP
                     RDMALPGLDLHLLKGERKGTWAVSVSGNWRITFAFSGPDVVNVDYEDYH"
     gene            2665..2952
                     /locus_tag="VN444_06815"
     CDS             2665..2952
                     /locus_tag="VN444_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWR21554.1"
                     /translation="MKMHNPPHPGEVLRELCLQPLGLTVTEAANALGVSRKTLSSILN
                     CRAGISPEMAVRLSIAFGTTAESWLSQQLQFDLAKAEAKRRSLRVKKLAAA"
     gene            complement(3075..3332)
                     /locus_tag="VN444_06820"
     CDS             complement(3075..3332)
                     /locus_tag="VN444_06820"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HWR21555.1"
                     /translation="MILDVVSARHIRDYELELEFEDGAKGIVDFSQYRSRGGVFSKFN
                     DMEYFKNFRIDKELGVISWENGVDIAPETLYSKAVGKAIAA"
     gene            complement(3343..3603)
                     /locus_tag="VN444_06825"
     CDS             complement(3343..3603)
                     /locus_tag="VN444_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HWR21556.1"
                     /translation="MPEISRFFGIIIAMFFDEHNPPHFHARYGSDKASIEIDSLRVLE
                     GSLPPRVLGLVIEWASQHKDELFANWEKVIQNKHPQKIEPLR"
BASE COUNT          796 a         1057 c          932 g          819 t
ORIGIN      
        1 gcgaatccgg ccacgtgagc cgacaccggt cgggatctca tggaagaggg cgaggaccag
       61 ttccttcagc cttggccgca cctcctcctt tgtcagctcc gtgcgaagca atcggacccc
      121 gcagttgatg tcgtacccga ctgcgccggg agacacgacg ccatcagtga tgtcggtggc
      181 aaccacccca ccgaccggca gaccgtaccc ctggtggata tccggcatag cgaaggaggc
      241 cttaacgatc cctggtagga aggcgccatt cgccacctgt tccagggaga ggtccttcat
      301 aatcgcctcc atcagggcct catcggcata gatgattcct ggaacccgca tccccggttt
      361 gtaatcttgc gggagcagcc atcgatagtc atcgagtttt tgtacgatct tactcatgcc
      421 caacctataa aggagattac tctaaaagct gataccgtgt gttgcggcgc ttcggaacta
      481 agcccgcatc ccggatgttt cgccggatct cctcgagcga catcagttgc ttggtgacgg
      541 cagtcctcac gacattctcc tcaaggacgg tactcccgaa atcgtttacg ccgtactgca
      601 gcgagagctg tccgattttc ggacccatcg tcagccaagc tgcctgaata tgttttacat
      661 tgtccagcat gatccttgag acggccacag tccgaagata gccgtagccg ctacctccgg
      721 tccctccgag cgcgtcgcca ttgggttggt agctccaagg aatgaaggct gtaaagccag
      781 cggtttgatc ttgtaactct cgaatttgga tgaggtgttc aacacgctga gccgacgtct
      841 cgcctacccc gtacatcatc gtggccgtgc tgggtatccc gagcccgtgt gcgatccgca
      901 tgaggttcaa ccagtcagac gaactctcct tcaacggcga aatccgctcc cttacctcat
      961 cgaccaacag ctcggctcca gctcccggaa tcgaatcgag ccccgccgct ttaagacgct
     1021 tcaaggtatc cttgatggat atcttagaac tatctgaaat ataactgatt tctaccgttg
     1081 aaagcgcgtg cagatgaacc ggatagcggg ccttgattga ggcgagcaga tcctcgtaat
     1141 agtcgatctt caggctaggg ttcagccccc cttgcagcaa aacctccgtc ccgccaagtt
     1201 cgatcagctc ctcgattttc tgaaagatct cctccttgga caggagatac gcttccggag
     1261 aggtcggcag tcgatagaag gcgcagaatg aacattggac ccagcagata ttcgtataat
     1321 taacggtgcg gccgatgaca taggtcacaa tcggctccgg atgcaagcgc cagcgtaccc
     1381 ggtcggccat ggcggagaga tcgagcagcg tcgcccgttc aaagagctcg accccttcat
     1441 cgaaggtgag ccgctccccc acctcaacct tctccgcaat cgcctcaatc ctgtcgttcg
     1501 attcagccat gccttcgatt cctcggctcc atccgacact cgttatggga cgcggtgcga
     1561 tttaccgcgt ccggctccac gctggagttg ggtcttcagt ttctcccaga actgtctggg
     1621 agagagaatc gccagattct cgacgtgacc tatatccagg agttcctggt cacccgtcac
     1681 gaacacatcg gctccagccg atatcgcagc cgacagaatg agtaggtcat cctgatcttc
     1741 tatgtcgacc gtcggcagtt ggccgggctg cgcaagcacg gtatcttgcc gcataagcca
     1801 aatgaagtct gcaatcaaat cctgagctac accgaacttt gtccgcaaga cacgcctcag
     1861 ctcgctcagc acttgtacgg aagtcaggag ttcatgtgaa gtcagaacct cgcgtagaac
     1921 gtctgcgcac agtccgcgag ttgccgctgc actggccagg acattggtat ccaggaaaac
     1981 cctcacgaca cctcggcgaa cacatcttcg tctgtcaggt aaccgcgtgc ctccgcaaaa
     2041 ggcatgatct tgcgccgcag tgcttcaaac tggctgatcc gaagctggcg acgaagggcc
     2101 tctctggcga cctcgctacg gtttcttcct gaccgacgac tcgccttggt taacaggtcg
     2161 tccagatctt tgtcgagtct gattgtgagg atgctcgtct tcatgtcata caatgtagga
     2221 caatttggct gtagtgtcaa tggtagtgtt cggcccaacg gtgttgcgga tcagccgcag
     2281 gcgaacgcca gagcttgctc gtgtggacga ctgtcaggtt gcaacccgct gtgagccggt
     2341 tgcaacctga cacgttacgt gttacacttc tcccgtggcg atcctgaagt tcaggcacaa
     2401 gggtttggag cgcttcttcc tccgtggaac gaccgtcggc atccaatcaa agcatgcccg
     2461 aagactacgg ctgatcctcg gtcgattgaa cgtcgcgagg gagccccgcg atatggccct
     2521 accgggactg gatctccacc tgctgaaggg cgagcgaaaa ggaacatggg ctgtgagcgt
     2581 gagtggaaac tggcgtatca cgttcgcgtt ctcaggccca gatgttgtca atgtcgatta
     2641 cgaagattac cactgaggtg ctccatgaag atgcacaatc cgcctcatcc gggtgaggta
     2701 ctccgcgagc tctgcttgca gccactcggt ttgacggtaa ctgaggcggc caacgcgctc
     2761 ggggtcagcc gcaagacgct atcaagcatc ctgaactgcc gcgcaggtat tagcccggag
     2821 atggcggtcc gtctgtcgat cgccttcggc accactgcgg agagctggct cagccagcag
     2881 cttcagttcg atctggcgaa ggcagaggcc aagcgccggt cgctccgcgt caagaagctc
     2941 gcggctgctt gactcccctt tccggctaac agagcgccgg atagaaagcg ggtggcgtct
     3001 caaccttgga accgtctacc agcctataac ggtcagcttc attgcccggt tcaactattt
     3061 cagatctcct tggactatgc cgcgatggct ttcccgacag ccttggaata gagcgtttcc
     3121 ggtgcaatgt cgacaccgtt ttcccaagag ataactccaa gctctttatc aattctaaag
     3181 ttcttgaagt attccatgtc gttgaatttg gagaataccc cgcctcgact acgatattga
     3241 gaaaaatcaa cgataccctt ggcgccatct tcaaattcaa gctcaagctc ataatctcga
     3301 atatgccgcg ctgaaaccac gtcaagtatc atggcgcacc tcctatcgaa gtggttcaat
     3361 cttctgtggg tgtttattct gaatcacttt ttcccaattc gcaaatagtt catctttgtg
     3421 ctgtgatgcc cactcaataa ccaaccccag caccctgggc ggcaaagatc cttccaatac
     3481 acgtaacgaa tcgatttcga tagacgcctt gtcgcttcca taacgagcat gaaagtgagg
     3541 cgggttatgc tcgtcgaaga acattgcgat gattattccg aagaatctcg aaatctccgg
     3601 catc
//
LOCUS       DATMXI010000352         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_311318, whole genome shotgun sequence.
ACCESSION   DATMXI010000352 DATMXI010000000
VERSION     DATMXI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311318"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..695)
                     /locus_tag="VN444_06830"
     CDS             complement(204..695)
                     /locus_tag="VN444_06830"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HWR21557.1"
                     /translation="MKTNRPVQRRAQTAPARCADIIEGSLQGLGLGRIIHYLALLRAW
                     DRTIASSIKERASVEDFRGGRLYLCVEDPIWLHELHMLRHKLKTILNEEVGEPAVGEI
                     VLRIGQARRCAPATPSNRGGQRARAVSPAGEASIAKLLSPLRDLPCGDAMQRFFQRWA
                     RSE"
     gene            complement(769..1224)
                     /locus_tag="VN444_06835"
     CDS             complement(769..1224)
                     /locus_tag="VN444_06835"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HWR21558.1"
                     /translation="MEEGLRLLIHIDGAARGNPGPAGIGVLLEAGNGPLQQGFCRYIG
                     EATNNVAEYEALLLALREARKFQPTVVEIRSDSQLLVRQIHGRYRVKNVRLIALHAQA
                     RDLINQLSALNCRFSIEHIGRELNHQADALANRAIDEALSEVPRERGRS"
     gene            complement(1226..1930)
                     /locus_tag="VN444_06840"
     CDS             complement(1226..1930)
                     /locus_tag="VN444_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HWR21559.1"
                     /translation="MLQDLEGLIAIQGLDAEIAEFDAAAAAIPVQIQRMEQQLAQAKA
                     ALDAATVEVERLHKLRRQKERELDEAGGELKKRQGRLYEVKTNQEYTATLKEVEGLKQ
                     KISALEEEVIIRLDDIDSAEKTRDHEEAKVRVTQEEFLKNKQQREDELLKLRSHLSTL
                     RKARERRSGSVETSLLQLYLRLLKSRDGLAVAQATGRSCEGCHVTLTHQLYNEVRRNE
                     EIRTCEGCGRILYWKE"
     gene            complement(2017..2093)
                     /locus_tag="VN444_06845"
     tRNA            complement(2017..2093)
                     /locus_tag="VN444_06845"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2057..2059),aa:Ile,seq:cat)
     gene            complement(2152..>2836)
                     /gene="rpoD"
                     /locus_tag="VN444_06850"
     CDS             complement(2152..>2836)
                     /gene="rpoD"
                     /locus_tag="VN444_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWR21560.1"
                     /translation="VVSIAKKYTNRGLQFLDLIQEGNIGLMRAVDKFEYQRGYKFSTY
                     ATWWVRQAITRAIADQARTIRIPVHMIETINKLTRASRYLVQEYGREPTPEEIAQKID
                     LPVDKVRKVLKIAQEPISLETPIGEEEGSHLGDFIEDKGVISPIEAVIGMNLSGQTEQ
                     VLETLTEREQKILKLRFGLGDGRDHTLEEVGQQFAVTRERIRQIEAKALRKLRHPTRS
                     RKLRSFVES"
BASE COUNT          538 a          876 c          747 g          675 t
ORIGIN      
        1 gctgatgact caccatgatc tcccgaccag atctgttctg tctaaggtaa tcctcactct
       61 cactcttacg aaccagccgc gatcttactc gaaggccctt gaaattgcaa gccgtatacc
      121 agttacttcg aaaatccctt caacccctct ttggtaaagg ggggcacggg gggatttcat
      181 gtggatatgt tgcaacgggg atgtcactct gatctcgccc accgttgaaa gaagcgctgc
      241 atggcatcac cacatggcag atccctgagg gggctcagga gctttgctat gctcgcctcg
      301 cccgcaggtg aaaccgctcg agctctctgc ccaccacgat tggagggtgt tgcaggggca
      361 caccgacggg cttggccaat tctcaacacg atctctccta ccgccggctc gccgacctcc
      421 tcgttcagga tcgtcttcag cttgtgacgc agcatgtgga gctcatgcag ccagatggga
      481 tcctcgacgc agaggtacag gcgtccaccc ctgaagtcct ccacgctcgc ccgctccttg
      541 atgctgcttg ctatggttcg gtcccaggct cgaaggagcg cgaggtaatg gatgatccga
      601 ccgagaccta gtccttgcag gctgccctcg atgatatctg cgcacctggc aggggcggtc
      661 tgcgctctcc tctgtaccgg ccggttggtc ttcacttagt aaactccact ggcggcgccg
      721 ctctcgccga ctcagctacc aggaagtagc gtacctgttg ctgtccgttc acgagcgtcc
      781 cctctcgcgg gggacttcgg ataaggcttc atcgatggcg cggttggcga gcgcgtccgc
      841 ctgatggttc agctcgcgac cgatatgctc aattgagaac cgacagttga gagccgacaa
      901 ctgattgatg aggtcgcgcg cttgcgcgtg caacgcaatg aggcgtacgt ttttgactcg
      961 atatctgcca tgaatttgtc tgaccaacaa ctgggagtcg gatcgaatct cgacaacagt
     1021 gggctgaaat ttcctggcct cccttaaggc aaggagcagg gcttcatatt cggccacgtt
     1081 gttggttgcc tcgccgatgt atcggcaaaa gccctgctgc aacggcccgt tccccgcctc
     1141 cagcaggacg ccgattccgg ctgggccagg atttccgcga gctgctccat cgatgtgaat
     1201 cagcagtcga agtccctcct ccatctcatt ccttccaata gaggatccgg ccacatcctt
     1261 cacaagtgcg aatctcctcg tttcggcgaa cctcgttata caactgatgc gtgagggtca
     1321 catggcaacc ttcgcaggaa cgacccgttg cttgagccac cgccaggcca tcccgactct
     1381 tgaggaggcg aaggtacaac tgcaataagg atgtctccac gctccccgac cggcgttccc
     1441 tggctttccg taatgtcgat aggtgactcc gaagcttgag cagctcatct tcccgctgct
     1501 gtttgttttt cagaaactcc tcctgtgtaa cccggacctt cgcctcctca tgatcccgag
     1561 ttttctcagc gctgtcgata tcgtcgaggc gtatgatgac ctcttcctcc agcgccgaaa
     1621 tcttttgttt cagcccctcg acctccttca gggttgcagt gtattcctgg ttggtcttga
     1681 cctcatagag acggccttgc cgttttttga gctctccgcc ggcctcatcc aactcgcgct
     1741 ctttctggcg gcgaagtttg tggagcctct ctacctctac ggtggccgca tcgagagccg
     1801 ccttggcctg agccaactgc tgctccatcc tctggatctg gacggggatg gccgccgccg
     1861 ccgcatcgaa ctcggcgatt tcagcgtcaa gcccctgtat tgcgataagc ccctccaagt
     1921 cctgaagcac gcttccctcc actatgatca gttgtcagcc caggataaag tcttaagctg
     1981 accgctgaaa gctaatagct gatagctgct tttacatggt gggcccacct ggactcgaac
     2041 cagggacgac ccggttatga gccgggggct ctaaccaact gagctatggg cccatgcaga
     2101 agaccagcaa ctagggagta gctatcagcg gtcagctatc agcccgctgc cctaagattc
     2161 cacgaagctc ctgagcttcc tgctccgagt aggatgccgg agcttccgta gcgccttggc
     2221 ctcaatctgt ctgatccgct cgcgggtgac agcgaattgc tggcctactt cctccaaggt
     2281 atgatcgcgc ccatctccga gtccgaaacg gagctttaag atcttttgct cccgttccgt
     2341 cagtgtctcg agtacctgct cggtctgacc gctcaagttc atgccgatga cggcctcgat
     2401 cggggagatc acgcctttgt cttcaatgaa gtcaccgaga tgagatcctt cctcctctcc
     2461 gatgggcgtc tccagagaga tcggttcctg agcaatcttc aggactttcc tgaccttatc
     2521 aaccggcaga tcgatcttct gggcaatctc ctccggggtt ggctctctgc catattcctg
     2581 aaccaggtac cgggaggcgc gggtcaactt gttgatggtt tcgatcatat gaacggggat
     2641 ccggatggtg cgggcctgat cggcaatggc tcgcgtgatg gcctggcgga cccaccatgt
     2701 ggcgtaggtg ctgaacttat atccccgctg gtattcaaac ttatccactg cccgcatcag
     2761 gccgatgttc ccctcctgga tcaggtccag aaactgcaga ccccggttcg tatacttctt
     2821 cgcgatgctc acgacc
//
LOCUS       DATMXI010000353         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_31950, whole genome shotgun sequence.
ACCESSION   DATMXI010000353 DATMXI010000000
VERSION     DATMXI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31950"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..1223)
                     /locus_tag="VN444_06855"
     CDS             complement(459..1223)
                     /locus_tag="VN444_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21561.1"
                     /translation="MREFASIGLAVLLTASGPSQAWAHGGGGGGITEGDLCSREKGHQ
                     LVHFSAYQRATAGAASQLAQLKELKDADLKRYVDALKEEFQSYCRDIPSTGKVTLTFD
                     LISDTLRTIPVTVRVVEVTNNGESGTILDIPQRVYPSGVVRAEAEFTKAGKYQAIVEE
                     HHADRAPIVSAAEAASHSHDGGGQKHASAGEEEAYHAYDSTFSFPFTVGLKTTRAGSS
                     SSMMSNPAILVMTIAGMGIGTVLYVRRKKKAAGEIQ"
     gene            complement(1467..2735)
                     /gene="amoB"
                     /locus_tag="VN444_06860"
     CDS             complement(1467..2735)
                     /gene="amoB"
                     /locus_tag="VN444_06860"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoB"
                     /protein_id="HWR21562.1"
                     /translation="MRIGRAALSAIVIGAIAVSAGTVWAHGERSQEPFLRMRTITFYD
                     TEWSKTKVKPGETMELTGKFHTFSEWPQAVNTPESIFLHYSVPGPSMLKKEAWMNGVP
                     VINSTSTVLGGDYDYKMVIAGRVTGTYHVHPMVNIAGGGPLVGGGQFVTVDGDWTGFQ
                     NNVATIDGTTVNMENHGLGRIIGWWLLWTVLGLFWLLYWVKRPFLRRVFQVGVVPEEQ
                     LVSSGDKTVAVVLMVATVLIVTVGYVSTNGAYPITIPLQAGRMETPELAPTTAFTPYA
                     HATVKARPITAVYTVPGRSLGFLVEIKNGSNRPQQLGGFITANLQFRDPNLFPDSRLK
                     IKVEPSGPIAPGQTVQLTVDATDAEWEYQRLAELIYDSDSRYGGLLEFFDPDKNRQIV
                     EVGGPVIPSFEGGATAMTGGGKWRPTTQYK"
     gene            complement(2856..3587)
                     /gene="amoA"
                     /locus_tag="VN444_06865"
     CDS             complement(2856..3587)
                     /gene="amoA"
                     /locus_tag="VN444_06865"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018597 - ammonia monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoA"
                     /protein_id="HWR21563.1"
                     /translation="MALLTQQQALSLERKFDIIVIIAAFTGTVAGYHIHQMLTVGDWD
                     FWLDWKDRRWWVTLTPILLITFPAATQYFMWEKMRLPIGATFCVMTLHFGQWMNRVFN
                     FYMWAWFPVNFVTPSLMIPSAIFLDVTLMMTGSYMFTALFGGMGWSLLFYPANWTWLA
                     PFHLAVKHPSGPLMSIADLMGMEYVRSATPEYIRIIERGTLRTFGRDVTPVSSFFAGF
                     ISGIVYLWWVWMGKVISRPVFLSRT"
     gene            complement(3700..4476)
                     /gene="amoC"
                     /locus_tag="VN444_06870"
     CDS             complement(3700..4476)
                     /gene="amoC"
                     /locus_tag="VN444_06870"
                     /EC_number="1.14.99.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial ammonia monooxygenase, subunit AmoC"
                     /protein_id="HWR21564.1"
                     /translation="MAQYKTEAVSGKSAESAEKVFGWGVFWKCMVPISIFYILIRIYQ
                     QLFSWSKGLDFFSEDFRVYWWNMLIGELLIEGAVLTFTLGYIWKTRDRNLDRITPEEE
                     LRRFWGLGMWIVTFAWAVYWGASFFTEQDGTWHQTVIRDTDFTPSHIIEFYLSYPIYI
                     IIGISSYMWARTRIPMFAAGHSLPFMLTVGGPAMIFVNVALNEWGHTFWIMEELFVAP
                     LHWGFVTLGWCLFGVYGIALTITPRVFELIKITSGGKSAA"
     gene            complement(4744..5160)
                     /locus_tag="VN444_06875"
     CDS             complement(4744..5160)
                     /locus_tag="VN444_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWR21565.1"
                     /translation="MAECVCSSIRKAARAVTELYDGILKPSGLRVTQFALLAVLASAG
                     STTITRLGQILAIDRTTMTRNLKPLVNQGLITIEAGQDQRTRVVTLTPSGREAVAKAL
                     PLWEKAQTRVVEGLGRKRWSTLLADLSAVMALARLK"
BASE COUNT         1165 a         1650 c         1338 g         1178 t
ORIGIN      
        1 gtgctgcgcc aggcgcccct tcgccgccgg cgccacctat cactaccggt ctggctgggg
       61 atatagccta gctcgcagcc tttcatattt tgcgggcggc tggcatcgga caacgcccca
      121 agcgatgttt tgcgcttaga gcgaactaac gaatcacccc gcaagcggct gggtgtcgcc
      181 tttctgttct ggcccgtcgt tccgtgcttg acacggaatc cagtcgggcc ctctggatac
      241 cggcttccgc cggtatgacg aacttgcggc aagccgcggg gaatgaacca ccagtggatt
      301 taataaactt ttagtccgct gcgaaaagtc tgtaggtgcg gacaaaggaa cttgttccta
      361 aagagttcct gcattacgat aggtgccacc agcaaaaaaa ttaaccctct cacgtgtttt
      421 catcttatcg tgagagggtt aattttttgc cggccttttt attgtatttc tccggctgct
      481 ttcttctttc gtcttacata gagcacagta ccgattccca tacctgcgat ggtcatcacc
      541 aagatagccg gattgctcat catggacgaa gaagagccag ccctggttgt cttcagtccg
      601 actgtaaagg ggaagctaaa ggtcgaatcg taggcatggt acgcctcttc ctcccccgca
      661 cttgcgtgct tctgacctcc cccatcgtga ctatggcttg ctgcctccgc agcactcacg
      721 atgggggctc gatccgcatg gtgctcctcg acgatggcct ggtatttccc ggccttcgta
      781 aactccgcct ccgctctcac cacgcctgag ggatagactc gctgcgggat atccaggatt
      841 gttccggact caccgttgtt cgtcacctca acgacccgga cagtcaccgg aatcgttcga
      901 agcgtatcgc tgatgagatc aaaggtcagt gtcacctttc ctgtactcgg gatgtctcgg
      961 cagtaggact gaaactcctc cttgagcgca tcaacgtacc gcttcagatc cgcatccttg
     1021 agctccttga gctgagcaag ctgagaggcc gctcccgcag tcgcacgctg ataggcgctg
     1081 aagtgaacca actgatgccc cttttccctc gagcaaagat caccctctgt gatcccaccg
     1141 ccaccgcccc cgtgggccca tgcctgcgat ggtccggatg ccgttaacag aactgccaaa
     1201 cctatcgacg caaactccct cattacccta ccctacttcc ctctcctgct cttcctgctc
     1261 aagctcccca cccacctggt gaggggggtg tcgattgaca gggtgcgggt cccacccgtt
     1321 gaaatccccg atccggggac tcaaaggatg gaaccctccc gcacccgctt atgcctggtt
     1381 caccaaagtc ataaggacaa caagcagcat tatcagtaga gctatgttca tcccggctgc
     1441 ttccatgatt gtgcctctcc ttctcgctat ttatactgcg tagttggccg ccatttgccc
     1501 ccgcctgtca tggcagtggc gccaccctca aaggacggga tcacaggacc gccgacctcg
     1561 acaatttgcc ggttcttgtc cggatcaaag aactcaagga gtccgccgta ccggctgtcc
     1621 gagtcgtaga ttaactcggc caacctctgg tactcccatt ccgcgtcggt agcatcaacc
     1681 gttagctgta ctgtctggcc aggagcgatg gggccggacg gctccacctt gatcttcagc
     1741 cgtgagtctg ggaagaggtt ggggtccctg aactgcaggt tggccgtgat gaaccccccc
     1801 aactgctgag gcctgttcga accgttcttg atctctacta gaaacccaag agaacgccca
     1861 ggcaccgtat agactgcggt gataggcctg gccttcactg tggcatgggc gtacggcgta
     1921 aacgccgtgg tgggcgctag ttcaggagtc tccatcctac cagcctgcag cggaatcgtg
     1981 atgggatagg cgccgttggt cgagacgtag ccaacggtca caatgaggac cgtcgcgacc
     2041 atgaggacga ctgccaccgt cttatcgccc gagctcacaa gctgctcctc aggcacaacc
     2101 cccacctgaa agacacgccg caaaaacggc cgcttcaccc aatataacag ccagaacagt
     2161 cccagcacgg tccacaggag ccaccagccg attatcctcc ccagcccgtg gttttccatg
     2221 ttcaccgtcg tgccatcgat agtcgcgacg ttgttttgaa agccagtcca atcgccatcg
     2281 acagtgacaa attggccgcc accgaccaag ggccctccac ccgcaatatt caccatcggg
     2341 tgaacgtgat aggtcccggt gacccgcccc gcaatgacca tcttataatc atagtctccg
     2401 ccaagtacag tactggtgga gttgatcacg ggcacgccat tcatccatgc ctctttctta
     2461 agcattgatg gccctggcac cgagtagtgg agaaagatcg actccggggt attgacggcc
     2521 tgcggccatt cactgaacgt atgaaacttt ccagtgagtt ccatcgtctc gccgggcttc
     2581 accttcgttt tcgaccattc agtatcgtaa aacgttatcg tgcgcatccg cagaaacggc
     2641 tcctgcgacc gctcgccatg ggcccatacc gttcccgcgg acacggcgat cgctccaatc
     2701 acgatcgccg acaacgccgc tcgtccgatc ctcattgtta tcctcccagg ttcgtgggta
     2761 agagtcaact cattcgcatc gcagcacccc gtggcgtctt ctcttgtctt gcagacttgc
     2821 ttcgagaacc caccacgagc ccgcatcgat gacagctacg tccgcgagag gaataccggc
     2881 ctgctgatca ccttccccat ccaaacccac cagaggtata ctatcccact gatgaacccg
     2941 gcaaagaacg aggagactgg cgtgacgtct cttccgaatg ttcgtagcgt cccacgctca
     3001 ataatcctga tgtattcagg cgtagcagac ctcacatact ccatccccat cagatcggcg
     3061 atcgacatga gcggcccgct tggatgcttc acggccaagt gaaacggggc gagccaggtc
     3121 cagttcgccg gatagaataa cagggaccac cccatgccgc cgaacagcgc cgtgaacatg
     3181 tagctgcctg tcatcatcag cgtcacgtcc agaaagatcg cgctgggtat catgagactc
     3241 ggggtgacaa agttaaccgg gaaccaggcc cacatgtaga agttgaagac ccgattcatc
     3301 cattgaccga agtgaagcgt catcacgcaa aaggtggcgc cgatcggaag acgcatcttc
     3361 tcccacatga aatattgcgt tgccgcgggg aaggtgatca ataggatcgg cgtcaacgtg
     3421 acccaccaac gccgatcctt ccagtccagc cagaaatccc agtcgccgac ggtcagcatc
     3481 tggtgaatgt ggtaccccgc gaccgtgccc gtgaacgcag caattatcac gataatatcg
     3541 aacttccgct ccaggctcag ggcttgctgt tgtgtcaaga gcgccatata gctcttcctc
     3601 tctctcaaag ggaggggggc acacagaacg ccacccccct ccactatttg gacttgtgga
     3661 cttcgaggac cgccaaaaaa cgatccttaa cgagtttcgt cacgcagccg acttaccacc
     3721 gcttgtgatc ttgatcagct caaagacccg cggagtaatt gtcagcgcta tgccatacac
     3781 cccaaaaagg caccagccga gcgtgacaaa gccccaatgc agcggcgcca caaataactc
     3841 ctccatgatc cagaaggtgt ggccccactc gttcagcgcc acattcacaa agatcatggc
     3901 aggaccaccg accgtcagca tgaaaggcag ggagtgtccg gccgcaaaca tggggattcg
     3961 agtcctggcc cacatgtagg agctaatccc gatgatgatg tagatcgggt agctgaggta
     4021 gaactcgata atgtggctcg gcgtaaagtc cgtatcgcgg atcaccgtct ggtgccacgt
     4081 cccgtcctgc tcggtaaaga agctggcccc ccaatagacg gcccaggcga acgtcacgat
     4141 ccacatgccg agaccccaga accgcctgag ctcctcctcc ggcgtgattc gatccagatt
     4201 ccggtcgcgg gtcttccaga tgtagcccaa ggtgaaggtc agcaccgccc cctcaatgag
     4261 caactcaccg atgagcatgt tccaccagta gacccggaag tcctcagaga agaagtcgag
     4321 gcctttcgac cacgagaaga gctgctggta gatccgaatc aggatgtaga agatgctgat
     4381 aggtaccatg catttccaga agacacccca gccaaacacc ttttcggctg actcggcact
     4441 tttacccgat accgcttccg tcttatactg tgccattgga ttcttcctcc ttacgctctg
     4501 atcaatttag attacgtggc tacactgttc tgggactgca ttgcacctta cctttttatt
     4561 gattccggct ctcacctcct ttgtcgccgc aatgaaacgg gttaaggttt acggccgttg
     4621 ttggtctcgt agaaacgttt cacggttggg ggggtcgtca agctgcacca cgggatcctc
     4681 ctttctcgac tattcagttc gccacataca tgagtgtgta tacacctata tgaccaaaaa
     4741 aactcacttt aatcgggcta gggccatcac tgctgacaaa tcggccagta acgtgctcca
     4801 gcgtttgcga cccagccctt cgacgacacg tgtttgagcc ttttcccaaa gcggcaaagc
     4861 ctttgccaca gcctcccggc cggagggtgt cagcgtcacc accctcgtgc gctggtcctg
     4921 gcccgcttcg atcgtgatca atccctgatt tactagcggc ttcaggttgc gcgtcattgt
     4981 agttcggtct atggccaata tctgaccgag gcgcgtgatg gtagtcgaac ccgcacttgc
     5041 gaggacggct agcaacgcga actgagttac ccgcaaaccg ctcggtttta gaattccgtc
     5101 atagagctct gtgactgccc gtgctgcctt gcggatactg gaacagacac actcggccac
     5161 ttaagccaac cctgactctg ttgggtaact cctcatattg agagtatatg cacctatcgc
     5221 tgacttgtca actcccgatt tacaattttt tcgggaggag gcgttgccgt cgctatccgc
     5281 ttcgactggg ccggccttta caggcgatac ggccttcggt atagtgcgcg t
//
LOCUS       DATMXI010000354         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_276256, whole genome shotgun sequence.
ACCESSION   DATMXI010000354 DATMXI010000000
VERSION     DATMXI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276256"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..2750)
                     /gene="ppdK"
                     /locus_tag="VN444_06880"
     CDS             complement(93..2750)
                     /gene="ppdK"
                     /locus_tag="VN444_06880"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWR21566.1"
                     /translation="MAKKYVYFFGKGRAEGRAEQKNLLGGKGSNLHEMTTLKIPVPPG
                     FTISTEACIEYVKRGRRFPPQLWAEIEADITKLERAMGKRFGDPKDPLLVSVRSGARI
                     SMPGMMDTVLNLGLNDETVQGLIERSQNPRFAYDSYRRLLQMFGDVVLGIKKIAFEGL
                     LSEKKRQKGVEADTALSAKDLKDLVEQFKGVIQRETGREFPRDPKEQLRMAVGAVFES
                     WNNKRAIEYRRIYKVPDNWGTAVNIQSMVFGNLGENSGTGVAFTRDPSTGEKRLYGEF
                     LPNAQGEDVVAGIRTPRHIVAMKKVWPQVYREFEQICGTLERHYRDMMDIEFTIEDGK
                     LYMLQCRVGKRTAHAAIKIAVDMAKERLIDQKVAVLRVEPWQLEQLLHPTIDPKAKVQ
                     KIAKGLSASPGAAVGKAVFTAEEAVEAVERHEKVILVRPETSPEDIAGMVAAQGILTA
                     RGGLTSHAAVVARGMGKACVVGCADIIVNEDEGYFQVANQTIRRGDLITIDGSTGNVI
                     LGEVALRQPEKLTGEFGTLISWADRFRKIGVRTNADTPRDARVTRQFGGEGIGLCRTE
                     HMFFEGDRVVAVREMILAKDADERRNALAKLLPMQKQDFKTMFELMDGLPVIIRTLDP
                     PLHEFLPRKDEEIRKVAEGMGVSEKLLKEKVNSLQEFNPMLGHRGCRLGISHPEITEM
                     QARAIFEAACELKKKGKRPFPEIMIPLVGHVKEFQIQRDIVKRVASEVMKAYGVKVRY
                     LIGTMIELPRACIVADQIGAVADFFSFGTNDLTQTAFGFSRDDSGRFLPQYLEEGILQ
                     ADPFAVLDEEGVGELMRIAIEKGRKAKPNLEIGLCGEHGGEPMSVELCHRLGLDYVSC
                     SPYRVPIAKLAAAHAVLKKQVKIKEMGEK"
BASE COUNT          528 a          900 c          711 g          640 t
ORIGIN      
        1 aagtagttta ggctgaaggc tgaaggctgt taggtaaatc ccttacgtct tgttaccttc
       61 agccttcagc ctatggactt gttttcctcg gcctactttt ctcccatctc tttaatcttg
      121 acctgcttct tcaggacggc atgggccgcc gccagcttgg caatggggac ccgatagggg
      181 gagcaactga cgtaatccag acccagtcga tgacagagct caacggacat gggctctcct
      241 ccgtgctcac cgcagaggcc gatctccagg tttggcttgg cctttcgccc cttctcgatt
      301 gctatccgca tcaactctcc caccccttct tcgtcgagca cagcgaaggg atccgcctga
      361 aggatccctt cctccaaata ctgagggagg aagcggccag agtcatcacg ggaaaacccg
      421 aacgcggtct gggtcaggtc gtttgtcccg aaggagaaaa agtcggccac cgctccgatc
      481 tgatcagcca caatacaggc cctcgggagc tcgatcatcg ttccaatcag atagcggacc
      541 tttacgccgt acgctttcat cacctcggag gctacccgct tcacgatgtc ccgctggatc
      601 tggaattcct ttacgtgtcc gacgagaggg atcatgatct ccgggaatgg gcgcttccct
      661 ttctttttca gttcacaggc cgcctcgaag atcgcccggg cctgcatctc agtaatctct
      721 gggtggctga tcccgaggcg acagccacgg tgcccaagca tcgggttaaa ttcctgcagg
      781 gagttgacct tttccttgag gagcttctcc gacaccccca tgccttccgc cacctttcga
      841 atctcctcgt ccttcctcgg caggaactca tgcaagggag ggtcgagcgt cctgatgatg
      901 acaggcaaac catccatgag ctcaaacatt gtcttgaagt cctgcttctg catcgggagc
      961 agcttggcca gggcgttgcg ccgctcatca gcatccttgg ccaggatcat ctctcgcacg
     1021 gcgacgactc gatccccctc gaagaacatg tgttcggtcc ggcagaggcc gatcccctcg
     1081 cccccgaact gtcgcgtaac ccgcgcgtcc cttggggtgt ccgcgttggt cctcacaccg
     1141 atcttccgga agcggtccgc ccacgagatc agggttccga actcaccggt cagtttttca
     1201 ggctgcctca acgccacctc cccgaggatg acattgcctg tgctgccatc gatggtaatc
     1261 aggtctcccc tccgaatcgt ctgattcgcg acctggaagt acccctcatc ctcgttgaca
     1321 atgatgtcgg cgcagccgac gacacaggct ttccccatcc cccgggcgac taccgcggcg
     1381 tgactggtga gaccacccct ggcggtgaga atcccctgcg cggcaaccat tcccgctatg
     1441 tcctccgggg aggtctcggg cctgaccagg atcaccttct cgtgccgctc cactgcttcc
     1501 accgcctcct ccgccgtgaa gacggccttc ccaaccgccg ctcccggcga ggccgagagg
     1561 cccttcgcga ttttctggac cttggccttc ggatcgatcg taggatgcag gagctgttcg
     1621 agttgccagg gctccactcg taggacggcc accttttggt cgatcaaccg ttccttggcc
     1681 atatcaacgg caatcttgat ggcggcgtgg gctgtccttt tgccgacgcg gcactgcagc
     1741 atatagagtt ttccatcctc aatggtaaac tcgatatcca tcatatcccg atagtggcgt
     1801 tcaagagttc cgcagatctg ctcaaactcc cgatagacct gaggccagac tttcttcatg
     1861 gccacgatgt gacgcggggt cctgatcccc gccaccacgt cctccccctg ggcattaggg
     1921 agaaattcac cgtagaggcg tttctcccct gtagacggat cgcgggtgaa ggccaccccg
     1981 gtcccggagt tctcccccag gttaccgaag accatagact ggatatttac ggcagtgccc
     2041 cagttatccg gaaccttata gatccgccgg tactcaatgg cccgcttgtt gttccaggac
     2101 tcgaatacgg caccgacggc cattcgaagc tgttccttcg gatctcgtgg aaactcccgc
     2161 ccggtctcac gctggatcac ccctttaaac tgttcgacca gatccttaag atctttggcg
     2221 ctgagtgccg tatcggcctc aacccccttc tgccgcttct tttcgctgag gagcccctca
     2281 aaggctattt tcttgatgcc cagtacgaca tccccgaaca tctggagaag cctccggtaa
     2341 ctatcgtagg cgaagcgggg attttgggaa cgctcgatga ggccttggac cgtctcgtca
     2401 tttagcccca agttcaatac ggtatccatc atgcccggca tagagattcg cgccccggag
     2461 cggacggaga ccaggagcgg atctttcgga tcgccgaacc gctttcccat agcccgctcc
     2521 agcttggtaa tatccgcttc gatctccgcc catagctggg gagggaaacg cctgcctcgc
     2581 ttcacgtact caatgcaggc ttccgtcgag atagtaaagc caggcggcac ggggatcttg
     2641 agggtcgtca tctcatggag gttcgatccc tttcccccga gaagattctt ctgctcagcc
     2701 cttccttccg cccgaccctt tccgaagaag tacacatatt tctttgcaat cgccatattc
     2761 tcccccatga gtgtgagta
//
LOCUS       DATMXI010000355         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_222754, whole genome shotgun sequence.
ACCESSION   DATMXI010000355 DATMXI010000000
VERSION     DATMXI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222754"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VN444_06885"
     CDS             complement(<1..1496)
                     /locus_tag="VN444_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HWR21567.1"
                     /translation="MNSQKRKLLLFLGAGASVDAGLPSAAMVTRHFESFSTHTCSSSA
                     VLVENLFRLVQVEIASQMGVSASSVDYEAVLGALLDAAEGRNLFSTKKLSELVHTHFG
                     QVDLQRLVRDYVLYIRGLFLPIRDVAYLDTLLHTVWEHEGTIATLNYDLCVERRHIEL
                     ERKVRTGFGPDRLWKGFDTEEPGTELLKLHGSVQWLRQSVTSDDPYSILHFLRFFSEP
                     FTTGLHKTAAWVADENGEGVRLLMNFGISKEQLYITPPFTEIFSRFHANLEEAGIIVI
                     AGYSFRDPAVNRMLLESLKHKRVPVVAINPQIDELMERHACVKALQEYGVLIPSSKSI
                     TKISRADVDQWLEVALKPAPASIASPPGFDDRAVVNRVHDLVVELSLLEYYIGDVLQE
                     TDAGHPAIYGQLNCLVQSLERCRGTYCQALMATGANIQPESARQVYSRELVNLLSCRE
                     ALADGLASLQNILRQLEPWQHPFQVISTKVKELDNDGNLRRLLEHILGT"
     gene            complement(1502..1768)
                     /locus_tag="VN444_06890"
     CDS             complement(1502..1768)
                     /locus_tag="VN444_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21568.1"
                     /translation="MDDAKTKIGQLIEKFEREQAAGRIYDYNESETKAGFIEPLLQAI
                     GWDTRDRNEVGFETKVSGGRVDYSLKLQGSPRIYVEAKPLSKKS"
     gene            complement(1817..2233)
                     /locus_tag="VN444_06895"
     CDS             complement(1817..2233)
                     /locus_tag="VN444_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWR21569.1"
                     /translation="MRPRLKYNPTLKQKARSLRTAMTDCEGIIWSRLRRKQVLGVQFY
                     RQKPLGHYIVDFYAPKAKLVVEVDGSHHATAEHMTKDGKRDADLAGRGIRVLRFNDLQ
                     VLQEIEAVMQEVFQAIAEQVERNPPTPPFCKGGSSE"
     gene            complement(2279..>2459)
                     /locus_tag="VN444_06900"
     CDS             complement(2279..>2459)
                     /locus_tag="VN444_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HWR21570.1"
                     /translation="SQTLNGILLPAVLVFMLLLINDRKIMGAQVNSPVYNLIAWGTAF
                     LMVVLTLLLLVTSIV"
BASE COUNT          586 a          663 c          620 g          590 t
ORIGIN      
        1 gtgcctaaga tatgctccag gagccttcgt aaattgccat cattatcaag ctccttaacc
       61 tttgttgaaa ttacttgaaa cggatgttgc cagggttcga gttgccgcaa gatgttttgt
      121 aaagaggcga gcccgtctgc caatgcctca cgacaggaaa gcagattgac cagttctctt
      181 gaataaactt gccgagccga ctcaggttgg atgttcgcgc cagttgccat cagtgcctgg
      241 cagtaggtcc cacggcatcg ctcaagtgac tggaccaagc agtttaactg cccgtatata
      301 gcaggatgac cggcatctgt ttcttgtaaa acatcgccaa tgtaatattc taaaagtgaa
      361 agctctacga ccaaatcatg gactctatta actacggcgc ggtcgtcgaa tcccggcggg
      421 gatgctatgc tggctggagc tggcttaagg gccacttcta accattggtc aacatcagct
      481 cttgagatct tcgtgatgga tttcgaagac gggataagga ctccgtactc ttgcaacgct
      541 ttgacacaag catgcctctc cattaactca tcgatctgag ggttgattgc caccaccggt
      601 acccttttgt gtttcaggga ttcgagtagc atgcggttga cggcaggatc acgaaaggaa
      661 tacccggcta tgacgatgat accagcctct tcgaggttgg cgtggaagcg agaaaatatc
      721 tctgtaaacg gaggagtaat atacaactgc tccttcgaaa tcccaaaatt catgagtagt
      781 cgaacaccct cgccgttttc gtcagctacc catgcggccg tcttatgaag gccagttgta
      841 aagggctcag agaagaaacg gagaaaatgt aagatggagt acggatcatc agaggtgaca
      901 gactgacgaa gccactgtac cgagccgtgc aattttaaca gttcggttcc aggttcttcg
      961 gtatcaaagc ctttccacag cctatctggt ccgaatccgg ttctgacctt tcgctccagt
     1021 tcgatgtgac gtctttcgac gcagagatca taattcaatg tcgctatcgt cccctcatgc
     1081 tcccagacag tatgaagaag ggtgtccaga tatgccacat cacgaatggg gaggaagagt
     1141 cctcggatat atagtacgta atcccgcaca agtcgttgaa ggtccacttg accgaagtgc
     1201 gtatggacaa gttctgatag tttttttgtg ctgaagagat tgcgtccttc tgcagcatca
     1261 agcagcgccc caagaacagc ttcgtaatca acgctgctgg ctgatacgcc catttgtgaa
     1321 gcaatttcaa cctggactaa ccgaaacaga ttctcgacta agaccgcgct tgaactgcaa
     1381 gtatgagtgg agaaactctc gaagtgccga gtgaccattg cagcggatgg tagcccggca
     1441 tctacagatg ctccagcacc caggaacaac aacagctttc tcttctgaga attcatctta
     1501 cttagctttt tttcgacagg ggtttggctt cgacgtagat ccttggggaa ccctggagtt
     1561 tcagcgagta atcgacccga ccacccgaga cctttgtctc gaatccgacc tcgtttcggt
     1621 cccgcgtatc ccagccgatg gcctgaagta gcggctcgat gaagccagcc ttggtctccg
     1681 actcattgta gtcgtagatc ctgccggcag cctgctcccg ctcgaacttc tcaatcagtt
     1741 ggccgatctt cgtcttcgcg tcgtccatat cagcgttctt aatcagaatt agaggcgtgc
     1801 actatcgccc tctcgttcat tccgagctcc cccctttaca aaaggggggc gtggggggat
     1861 ttcgctccac ctgctccgct atcgcctgaa agacttcctg catcactgcc tcgatttcct
     1921 gcaacacctg caagtcatta aatcgaagaa cgcgtatccc acgccccgcc aagtcggcat
     1981 cgcgcttgcc atctttggtc atatgctcag cagtcgcatg gtgggaaccg tcaacttcca
     2041 cgaccagctt cgccttgggc gcataaaagt ccacgatata atgcccgaga ggcttctgtc
     2101 ggtagaactg cacacccagc acctgtttcc gacgcaggcg agaccagatg atcccctcgc
     2161 agtcggtcat ggccgtccgt agactgcgcg ccttctgttt cagcgtagga ttgtatttga
     2221 gccggggcct catgaaatcc cccctaaccc ccctttggta aagggggagg atcataggtc
     2281 acacaatgct ggtcaccagc aacaggagcg tgagcacgac catcaggaag gcggtccccc
     2341 aggcgatcag gttgtagacc ggtgagttca cctgcgcgcc catgatcttc cggtcgttga
     2401 tcagcaggag catgaagacc aggacagccg ggagcaggat gccgtttaag gtctgggag
//
LOCUS       DATMXI010000356         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_72957, whole genome shotgun sequence.
ACCESSION   DATMXI010000356 DATMXI010000000
VERSION     DATMXI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72957"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VN444_06905"
     CDS             <1..534
                     /locus_tag="VN444_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21571.1"
                     /translation="LVQRGPSFPDQIDVSINRGTAEYKVRSKAGKDGKEEVLTGAFDL
                     PKDVYNGMFVTLLLNLPKGASETVNFLAFTPRPEVIKLELLLMGEHTVRIGNLTQKAV
                     QYVFKPDIGMIRELFGKATGKIPAQFHYDCWILSDEVPSFVQFEGPLQLMGPIMRIEL
                     VSPHLSIQPEDEKTSSK"
     gene            619..1488
                     /locus_tag="VN444_06910"
     CDS             619..1488
                     /locus_tag="VN444_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWR21572.1"
                     /translation="MDLQLCYTASEDTFPLPRPFKECYGRFGFPAPADAHRPYISSNF
                     VMGLDGRASFRELEDRADGNVVSRSREDRWLMDFLRAHHDAQLIGASMLREELNPAGR
                     GWDYGIDDEELRIYRQDALHLGRQKVLVLSGSGNIDLTLRLFSSPRVEAWIITAREGE
                     KNLLSQLKKLGREGTIKILSVGEGTRVDLVAATQLLRQKHGIRTLLCEGGPMLYGEFV
                     KNQLVDEDFRTMSFQVLGESTKPGIDRPTAYGHVSYTPETAPWFRLISLHYALPYHAF
                     FRLRYEGPRTFQD"
     gene            complement(1586..1942)
                     /locus_tag="VN444_06915"
     CDS             complement(1586..1942)
                     /locus_tag="VN444_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWR21573.1"
                     /translation="MEIPMKLADKKCSDGVSVPPLKILRSRELLKKLGHGWKLNGKGH
                     LERRYTFKDFAQALAFTNKVGAVAEAEGHHPDLYLAWGKCKIEIWSHKIKGLTESDFV
                     LAAKAEQKFKPFRAPA"
BASE COUNT          471 a          561 c          559 g          471 t
ORIGIN      
        1 ctggtccagc gcggcccttc gtttccggac cagatcgacg tctcgatcaa ccgaggcaca
       61 gcggaataca aggttcgatc aaaagcgggg aaggatggga aggaggaggt gttgaccgga
      121 gcgtttgatc tgcccaagga tgtatacaac ggtatgtttg tcacgctgtt gctgaacctg
      181 cccaaaggtg ccagcgaaac ggtgaacttt ctcgccttca cgccgaggcc agaggtcatc
      241 aaactggagc tgcttctgat gggtgagcac accgtccgaa taggcaacct gacacagaag
      301 gcggtgcaat acgttttcaa gccggacatc ggaatgattc gtgagttgtt cggcaaggcc
      361 accggcaaga tcccggccca gtttcactat gactgttgga tcctgtccga tgaggtgccg
      421 agtttcgtgc aatttgaagg gcccttgcaa ctcatggggc cgatcatgcg aatcgaactt
      481 gtgagccccc acttgtccat acagccggaa gacgagaaga cttcttcaaa atgacgttac
      541 gacatcttgc atccctgttc agacttgtat tcaagcaccg catggtgaat atcatactgt
      601 caaattttgg agacgccgat ggacctgcaa ctttgttata ccgccagcga ggataccttt
      661 cccttacctc gacccttcaa ggaatgctat ggccggtttg ggtttcctgc acctgccgac
      721 gcccatcgtc cgtatatctc ctccaacttt gtgatggggc tcgacggcag ggcttcgttt
      781 agagagttgg aggaccgtgc cgatggtaat gtcgtttcaa ggagcagaga agatcgctgg
      841 ctgatggatt ttctgcgggc gcatcatgat gcccaattga tcggtgcgag tatgctccgc
      901 gaggaactga accctgcagg acggggctgg gactacggta tcgatgacga ggaattgcgg
      961 atataccgac aagatgccct ccatctcggc cggcaaaaag tcctcgtcct cagcggttcc
     1021 ggaaacatcg atctcactct tcgcctcttc agctctccgc gggttgaagc ctggatcatc
     1081 accgcgcgcg agggagaaaa gaatctcctg tcgcagctca aaaagctagg ccgtgaggga
     1141 acgattaaaa tcctctccgt tggtgaagga acgagggttg atcttgtcgc ggccacacaa
     1201 ctgctcaggc aaaaacatgg catccggaca ctgctctgtg aaggcggacc gatgctgtat
     1261 ggagagttcg tcaagaacca actagtcgat gaggactttc ggacgatgtc gtttcaggta
     1321 ttgggtgagt ccaccaagcc ggggatcgac cgaccgacgg cgtacggtca cgtgagttat
     1381 acgccagaaa ctgcgccgtg gtttcgcttg atcagccttc actatgctct gccgtaccac
     1441 gcattcttcc gccttcgata cgagggtcca agaacgtttc aggactgagt ctctagggta
     1501 agaacgtcgt cgaggcgcta cgaccggagt agccggtagc gaaatcttcg cgcccccttc
     1561 acccacgagc ccggactcct gtcggctatg ctggtgcgcg aaacggttta aacttctgtt
     1621 cggctttggc cgccaagaca aagtcgctct ccgtcaggcc cttaatcttg tgtgaccaga
     1681 tctcgatctt gcatttcccc caggccagat atagatcagg atgatggccc tcggcctccg
     1741 cgacagcgcc aaccttgttc gtaaacgcca gcgcctgcgc gaagtccttg aaggtgtagc
     1801 gccgctccaa atgccctttt ccgttgagtt tccagccatg gccaagtttc ttcaataact
     1861 ccctcgagcg aagaatcttc aacgggggca cactgactcc atcgctacat ttcttgtctg
     1921 caagtttcat cggaatctcc atgaatagcc agagcaattg atagtaattg actctggttc
     1981 aggtgaatct gttggtcagg atccccggac agagctactc gagaacagat ggatcgccag
     2041 caatacgact ccgatcaaga ac
//
LOCUS       DATMXI010000357         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_32286, whole genome shotgun sequence.
ACCESSION   DATMXI010000357 DATMXI010000000
VERSION     DATMXI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32286"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VN444_06920"
     CDS             complement(<1..1028)
                     /locus_tag="VN444_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR21574.1"
                     /translation="MYKTIYVPVDNSDHSNTAVDVGVHFAKAFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDYVDKKCAENNLPI
                     ERRSLEGRNWKVLAEDINTNAYDLVIMGALGVGAVKDSVIGSNTERVLRRVRNSDMLI
                     IKQTQPMTGGKIVVAVDGSPYSFGGLMTGLALGKAFNMPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEIIDSGLAKIYQSHLDICREIAQAEQTDVKTTLLDG
                     KAFEKIIQYVRKDIPALLIVGRIGVHSDEDMDIGSNTENLLRSAPCNILVSNRKYVPP
                     IDTLAEYT"
     gene            complement(1186..>2471)
                     /locus_tag="VN444_06925"
     CDS             complement(1186..>2471)
                     /locus_tag="VN444_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR21575.1"
                     /translation="IRAALSYPWLIAKEFPKAQIMIGGGAFTAFADQLIEKLPEGTIG
                     ILGEGEDAILKVIEGQSLEKERYIIREGKTIRKGQQGSPALLDALTVDLPYVTSIFPQ
                     HREYLGESIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPPSMVVKDISQHYHQWGARRF
                     WFTDAQFITGKEAYPQCIEILERILAEKLEIEWSGYIRTSLITPELAKLMVRSGVGDL
                     EVAITSGSQVVLNDLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQ
                     SVESYKVVASILGEERVFPLMFFLGIQPNTDLEQRLLEEGYLSAGYNPLMLTPTSIRK
                     LLYNPAPLNSFIAKACLRAWERKEGSRDPRKWTGSLSQSSEGSGPYADKSLIKGIEGN
                     SGRDALLSLEEILRSRPSFRSEKNSGEKSVITPAS"
BASE COUNT          531 a          679 c          665 g          596 t
ORIGIN      
        1 atcgtatact cagccagcgt gtcgatcggc ggcacgtatt tgcgattcga taccaggata
       61 ttgcagggcg cagaccgcaa cagattttcc gtgttgctgc cgatgtccat atcctcgtcg
      121 ctgtgtacgc cgatccgtcc gacgatcagg agcgccggga tatccttgcg aacatattga
      181 atgatctttt cgaacgcctt gccatccaac agagtggtct tcacgtcggt ttgttcggcc
      241 tgagcgatct cgcggcagat gtcgaggtgc gattggtaga tcttcgcgag gccgctgtcg
      301 atgatttctt catgtagctt ttcctgctct ttgaagcgga aaaccttgcc ggcctcttcg
      361 ttgagcacac ctgaaatact atgaaaggcg gcatagtgga aatagggatc gaaggccgag
      421 atggcctcga ccggcatgtt gaaggccttt cccagtgcca ggcccgtcat gagtccaccg
      481 aaggaatagg ggctgccgtc caccgccacc acgattttcc caccggtcat gggctgagtt
      541 tgtttgataa tcagcatgtc agaattccgc acccgccgaa gcactcgctc ggtgttactg
      601 ccgatgacgc tgtccttcac cgcgccgaca cccaaggcac ccatgatgac caggtcgtag
      661 gcattggtgt tgatatcttc agccaacacc ttccagttcc ggccttcgag cgagcgacgc
      721 tcaatcggca ggttgttttc cgcgcatttc ttatcaacgt agtcgagata ggaatcggtg
      781 atgatttgga ggcctcgggt gatcaacgaa tcgtgaatct ggcgctgtcg gtcgagttct
      841 ttttcatcgt ggtattcctc cggcaagccc gcttccatct gtttgaagcg cttgtcatgc
      901 attttggctg catagacgtg gctccccacg atcttcgagc cgaaggcctt cgcgaaatgc
      961 actccgacat cgacggcggt attggaatgg tcggagttat cgaccgggac atagatcgtc
     1021 ttatacatcg aaagatctcc tccagatgcc ctccaggccg gggcgaacga gagtaattat
     1081 caaaatttcc aaacggttga cgtacttccc cagcgtaaag gacgctcatg ctagcaccgc
     1141 tctgaattcg aaagcaaggc gtcaacgagg gcgctggggc aatatctagg atgccggggt
     1201 tatgacggac ttttcgcccg aattcttttc ggatcgaaat gagggtcggg aacggaggat
     1261 ttcctcgaga gacagaaggg catcgcgtcc ggagttccct tcaatccctt tgatcaaact
     1321 cttgtcagca tagggtcccg acccttctga ggattgggag agggatccag tccacttccg
     1381 tgggtccctg cttccttcct tccgttccca ggccctgagg caggccttgg caatgaacga
     1441 gttcaacggc gccgggttgt agaggagctt tctgatgctc gtgggagtca gcatgagagg
     1501 gttataaccg gccgaaagat acccctcctc caacagccgt tgttccaagt cggtattcgg
     1561 ctgaattcct aaaaagaaca tgagtgggaa gacacgctcc tcacccagga tcgaagcgac
     1621 caccttgtag gattccacgc tttggagcag tgactcctcc gtttcttttg gactgttcaa
     1681 cgagtaattg aggatgacct ttcctttgaa tcccgcttct gccagatagc gacagccatc
     1741 gtacagccgc tccagtttga atcccatgtg gaggtcgttc agcaccactt gcgagccgga
     1801 tgtaatggcg acctcaaggt cacccacgcc ggaccggacc atgagcttcg ccaactccgg
     1861 ggtgatgagg gatgtcctga tataacccga ccactcaatc tccagctttt ccgccagaat
     1921 ccgttcaagg atctcgatac actggggata ggcttctttc cctgtaataa actgagcatc
     1981 cgtgaaccag aatcgcctgg caccccattg atggtagtgc tgagagatat ccttgacgac
     2041 cattgaaggt ggtcgatagc ggaccctctt tccttcgatg taggggtaga gacagaacgc
     2101 acagtcgtag ggacaacctc gctttgactg aaccccaatg gattcaccaa ggtattcgcg
     2161 atgttgcggg aagattgacg ttacgtatgg caaatcgact gtgagcgcat cgagtagggc
     2221 cggcgacccc tgctggccct ttcgaatggt cttcccctcc cggataatgt agcgctcctt
     2281 ctccagcgat tggccctcta tgaccttgag tatggcatct tcgccttccc cgagaatccc
     2341 gatggtgcct tcggggagtt tctcgatcag ctgatcggca aaggcagtaa acgcaccccc
     2401 tccgatcatg atctgagctt tggggaattc cttggcgatc aaccaggggt aggagagggc
     2461 cgccctaata t
//
LOCUS       DATMXI010000358         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_249769, whole genome shotgun sequence.
ACCESSION   DATMXI010000358 DATMXI010000000
VERSION     DATMXI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249769"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN444_06930"
     CDS             <1..250
                     /locus_tag="VN444_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744316.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWR21576.1"
                     /translation="GTFLGILMSYGVIQPLAQNLEHQAQCEGKYLACIKTCLVAFARG
                     APPIVAVEFGRRVIFSYDRPSNQEMESACKGTPIKEAT"
     gene            247..1173
                     /locus_tag="VN444_06935"
     CDS             247..1173
                     /locus_tag="VN444_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWR21577.1"
                     /translation="MSEAPEQDNGGVRKIIKKRGGHGGHHGGAWKVAYADFVTAMMAL
                     FIVLWIVGQSKSTKEAIAGYFKDPSNFSKGGSPGGLQSKGSMGLLSKGGDPVMVAQQA
                     SDTIPHEELEAIQKSPEDKEQDRVHLEEAAEQFRQVIQRVPTLQALQGQIRIEITSEG
                     LRVQLIEGNRDSFFDLGSSRLKPVTRQLLAAIAHEVSKLPNQVVVEGHTDARPYSGNT
                     GRDYSNWELSTDRANSARRTMEEAGLRRNQVSRVIGYANQHLFNPTDPLDMANRRISI
                     LVPYLAAPAEASAPSTGLRTGLPADPATKGKS"
     gene            complement(1201..>2651)
                     /locus_tag="VN444_06940"
     CDS             complement(1201..>2651)
                     /locus_tag="VN444_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR21578.1"
                     /translation="VADDEKNLRLTLTDALQREGHTVLTAADGAETIDICQRLSPDLI
                     LLDLILPDVNGIQLLKRIWRVGGPPIIIMTAYGEVRSAVEAMKHHAYDYICKPFDLDE
                     LRLILHRVLEEVRTRQEVERLHGIGEGAYRCGVFVRKSEAANALWQVVKRVAASSTRI
                     VLLSGETGTGKELVAKALHYESQRRAHPFVDVNCATIAENLFERELFGHERGAFTDAK
                     GAKRGLAEVSHHGTLFLDEIGEMAMGLQAKFLRFLEEWKFRRVGGVHDLHVDVRVVAA
                     SNRDLKGLVAKGKFREDLFYRLNAIQIAIPPLRERRADILPLAAFFLQSSNTVFGKKI
                     RGLTPDVERRFERYDWPGNVRHLRNVVDYLVMMETEEFIYPIHLPSEITESGVTRETS
                     IIDAPAAPLMPCSSDQAWDRPVGGAKGNPLHSLAEVERAYIDRILKEVGGNKTEAAKV
                     LGISRQTLRTKLTHDSQHDSGSRSKTITPDAD"
BASE COUNT          566 a          815 c          758 g          512 t
ORIGIN      
        1 cggcacgttc cttggcatcc tgatgtcgta cggcgtcatt cagccgctgg ctcagaacct
       61 cgagcaccag gcgcaatgcg aaggcaaata tctcgcctgt atcaagacct gcctggtcgc
      121 atttgcgcgc ggcgcgccgc cgatcgttgc cgtcgaattc ggtcgtcggg tcatcttcag
      181 ttacgaccgg ccgtcgaacc aggagatgga gtcggcctgt aaggggacac ccatcaagga
      241 ggcaacatga gcgaagcgcc ggagcaggac aacggcggag tacgtaagat catcaaaaaa
      301 agaggtggcc acggcggcca tcacggagga gcctggaagg tggcctatgc cgacttcgtg
      361 accgcgatga tggcgctatt catcgtacta tggatcgtcg gccagagtaa gtcgaccaag
      421 gaggcgatag ccggatactt caaagaccca tccaatttct cgaagggcgg ctcacctggc
      481 ggcttgcagt ccaaggggag catggggctc ctctctaaag gaggcgatcc ggtcatggtc
      541 gctcagcagg caagcgacac aatccctcat gaggagttgg aagcaatcca aaaatcgccc
      601 gaagataagg agcaagatcg ggtccacctc gaggaggcgg cggaacagtt cagacaggtc
      661 atccagcgcg tcccaacact gcaagcgctt caagggcaga ttcgaatcga gatcaccagc
      721 gaggggcttc gcgtccaact gatcgaaggc aatcgggaca gtttcttcga tctcggctcg
      781 tcccgactga agccggtaac ccgtcaactg ctggcggcca tcgcgcatga ggtgtccaaa
      841 ctccccaacc aggtggtcgt cgaagggcac actgatgctc gcccgtacag cgggaatacc
      901 ggacgggact actcgaactg ggagctctcg acagaccgag ccaatagcgc ccggcgcacg
      961 atggaggagg cgggactccg acgtaaccag gtctccaggg tgatcgggta cgcaaaccaa
     1021 cacctgttta atccaacgga cccgctcgat atggccaatc gccggatcag tatcctggtg
     1081 ccgtacctcg ccgcaccggc tgaagcctcc gccccttcga caggactcag aacaggctta
     1141 ccggccgatc cggccaccaa ggggaagagc tagcagaaga aggatgggag aacccggccg
     1201 tcagtcagcg tcaggtgtga ttgttttgct gcgactgccg gaatcgtgtt gagaatcatg
     1261 ggtgagctta gttcgcagtg tctgccgtga gatccccagt acctttgccg cctctgtctt
     1321 gttcccgccg acttccttga ggatccgatc gatataggcc cgttccacct cggccagact
     1381 gtggagcgga ttacctttgg cgccaccgac cggtctgtcc caagcttggt cggaggaaca
     1441 gggcataagg ggagcggcag gcgcgtcgat gatcgaggtc tctcgggtta ctccggattc
     1501 ggtaatctcc gacggcagat gaatcgggta gatgaactct tctgtttcca tcatcaccag
     1561 ataatcgaca acattcctga ggtgccggac attccccggc caatcgtacc gctcgaatcg
     1621 ccgctcgaca tccggcgtca accctcgaat cttcttccca aagacggtat tgctcgactg
     1681 gaggaagaat gccgccagcg gcaagatgtc ggctcgacgc tcacgcagag ggggaatagc
     1741 aatctgtatc gcgttcagtc ggtagaagag atcctcccgg aacttccctt ttgccaccaa
     1801 ccccttgagg tcgcggttac ttgccgccac tacccgcaca tcgacatgca gatcatgcac
     1861 ccccccaacc cgacgaaact tccactcctc gaggaagcga aggaacttcg cctgaagtcc
     1921 catcgccatc tcgccgatct cgtccagaaa gagcgtccca tggtggctta cctccgccag
     1981 acctcgcttg gcgccttttg cgtctgtaaa ggcgccgcgt tcgtgcccga acagctctct
     2041 ctcgaacagg ttctcggcga tggtggcgca gttgacatcg acaaagggat gcgcgcgccg
     2101 ctgcgattcg tagtgcaggg ctttcgctac cagctccttt ccggtcccgg tctcgccgct
     2161 gagtaagacg atacgggtcg agctggcggc aactcgcttc actacctgcc acaacgcgtt
     2221 ggccgcctcg gatttcctga caaatacccc gcatcggtat gccccctcgc cgatcccgtg
     2281 caggcgttcc acctcctgac gggtccgcac ctcctccagt acacgatgca ggatcagcct
     2341 cagttcatcc agatcaaacg gcttacagat ataatcgtag gcgtggtgtt tcatcgcctc
     2401 tacggcgctc cggacctcgc cgtaggcggt catgatgatg atcggtgggc cgccgacccg
     2461 ccagatccgt ttcagaagct gaataccgtt cacgtccggc aggatgaggt cgagcagaat
     2521 cagatccgga gagagtcgtt ggcagatgtc aatcgtctcg gcgccatccg cagccgtgag
     2581 cacggtgtgt ccttcccgct gcaaggcgtc ggtcagcgtc agcctcaaat tcttctcatc
     2641 atccgctacg a
//
LOCUS       DATMXI010000359         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_292747, whole genome shotgun sequence.
ACCESSION   DATMXI010000359 DATMXI010000000
VERSION     DATMXI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292747"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1066)
                     /locus_tag="VN444_06945"
     CDS             complement(128..1066)
                     /locus_tag="VN444_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWR21579.1"
                     /translation="MTFHQLRIFLAVARHGSYSRAAEELLLTQPAVSAQVRELERALD
                     ATFFERVGRTIALTEAGKELQAYAERICVLIDEARLAMEELDGLKRGRIAVAAVSTAG
                     AYVLPSLLGAFQKRYPGISINLEVTNRALARDRLLHNEVDLVVMGRPPEEVPHVAEPF
                     LADEIVVVAAPSHPLATAKRISVDHLAREVFIAREVGSGTRLNTDEFFRHQGVKLRVG
                     LELGDNSAVKEAVAAGLGIALLSRHVLRMELKLKRLVVLDVQGLPLRRQWFVVHREDK
                     HLSRAAIAFKAFLLTSAEAVLASTSHPRLTKGRSRH"
     gene            1284..2183
                     /locus_tag="VN444_06950"
     CDS             1284..2183
                     /locus_tag="VN444_06950"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWR21580.1"
                     /translation="MTEPIVLRTDFSGLTLYARGKVRDIYDFGDRLLLVATDRISAFD
                     VVLPTGIPGKGRVLTALSAFWFHMTADLAPNHLITTDVEAYPKACQPYRETLEGRSML
                     VRKTKPLPIECIVRGYLSGSGWAEYQKTGAVCGIPLPAGLLESCRLDPPLFTPSTKAE
                     QGAHDANITFDEAAAQLGVELAERVRDLSLALYERARAYALERGIIIADTKFECGLLD
                     GNLILIDEVLTPDSSRFWPCDTYTPGRSQPSFDKQFVRDYLKSIAWEMKAPAPELPCE
                     IVERTSEKYREALRLLAGPTSHA"
     gene            2176..2445
                     /locus_tag="VN444_06955"
     CDS             2176..2445
                     /locus_tag="VN444_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWR21581.1"
                     /translation="MHSFVISTLKQARRAIVIVIGFTILVLGIVLIVLPGPAILVIPL
                     GLAILATEFVWARRLLVRFRWKARRLKNTISRKVQGDRRENSRSM"
BASE COUNT          535 a          748 c          782 g          535 t
ORIGIN      
        1 tcgggatcaa tcgcaacacg ctccggaaga aactccgcga gcttggaatc acgcctggcc
       61 gcggcgacga agaaaagaca gaaggatgag ctttacagtg acactgcggc gtgagcgtcg
      121 cttccgttta gtgacgcgac cgacctttgg tgagccgagg gtgactggtg gatgccagca
      181 ccgcttcggc cgaggtaagg aggaacgctt tgaaagcgat tgctgctcga ctgagatgct
      241 tgtcttcccg gtgtaccaca aaccactgcc gccgcagggg aagcccctga acatccagca
      301 cgaccagacg cttaagcttt aactccatgc gaaggacatg acgggatagg agcgcgatcc
      361 cgagcccagc ggccacagcc tcctttacgg cgctattatc ccccagctcc aacccaaccc
      421 gcagctttac cccttgatgg cggaagaatt catcggtatt gagacgggtt ccggagccta
      481 cctcgcgcgc gataaagacc tcccgggcca ggtgatccac tgaaatccgc ttggccgttg
      541 cgagcggatg agatggggcg gcgacgacga cgatctcgtc agcgagaaac ggctcggcca
      601 catgcggaac ttcttcagga ggacgtccca tgacgacgag gtcaacctca ttgtgcagga
      661 ggcggtctcg cgcaagtgcg cggttggtga cctccaggtt gatactaata ccaggatatc
      721 gcttctgaaa tgccccgagc aatgagggca ggacataggc gccggcagtg ctcacggcgg
      781 cgacggcaat tcgtccgcgc ttcagaccgt cgagctcttc catcgcaagc ctggcttcgt
      841 cgatcagtac acagatcctt tcggcgtaag cctggagttc cttgcccgcc tcggttaagg
      901 caatggtccg gccaacccgc tcgaagaagg tcgcatcgag ggcccgctcc agctcccgaa
      961 cctgcgccga gacggcaggc tgggtcagga ggagttcctc ggctgcgcgg gagtagctgc
     1021 catgtctggc gaccgccaga aagatgcgaa gctgatgaaa ggtcataatg attattccta
     1081 tcgctgtgct tgcgtgcaat tataaacaat tgtccttaga tacttcagaa atttcttatt
     1141 tgactttatg ttgagagcgt tgcacactgc gtaatacttg agcgaatacc gtgaagccgc
     1201 gatggtttca cgcctgaatc ttcaggcgta gcgcacgatt gtgcgtgtaa ttgcggaggg
     1261 tagccgaaag gatctcgatt gacatgaccg aaccgattgt attgagaaca gacttctcgg
     1321 gcctcaccct ctatgcgagg gggaaagtca gggatatcta cgacttcggc gatcgcttgc
     1381 tgctggtagc cacggaccgg atctcagcgt tcgacgtggt tctgccaacg ggaattccgg
     1441 gaaaggggag ggtcctcacg gccctctcag cgttctggtt ccacatgaca gcggatctgg
     1501 cgcccaacca cctcatcacc acggatgtgg aggcttaccc gaaggcctgc cagccctacc
     1561 gggagacgct tgaggggcgg agtatgctgg tgaggaagac gaaacccctc cctattgagt
     1621 gcattgtccg gggctacctc tctggatcgg gctgggcaga gtaccagaag accggagccg
     1681 tctgcggcat cccgttacct gccggcctcc tggagtcctg ccgtctcgat ccgcccctct
     1741 tcacgccttc cacaaaggcg gagcaggggg cgcacgatgc caacatcact tttgacgagg
     1801 cagccgctca gcttggcgtg gagctggccg aacgggtgcg ggacttgagt ctggcgctgt
     1861 atgagcgggc cagggcttat gctttggagc gggggattat catcgccgac acgaaattcg
     1921 agtgtggatt gcttgacggc aacctcatcc ttatcgacga ggtgctcacc cctgactcct
     1981 cccgtttctg gccttgcgat acctacacgc caggccgatc ccaaccgagc ttcgacaagc
     2041 agttcgtgcg ggactacttg aagtcgatcg cctgggagat gaaggcgcca gcccccgagt
     2101 tgccttgtga gattgtggag cggaccagtg agaagtaccg ggaagccctc aggctgctag
     2161 cggggccgac ctctcatgca tagctttgtc atcagcacgc tgaaacaggc caggcgcgcc
     2221 atcgtcatcg tcataggttt tacgatcctg gtgctcggca tcgtcctcat tgtactcccc
     2281 gggcccgcta ttctggtcat cccgcttggc ctggcaatcc tggccactga gttcgtctgg
     2341 gccaggagac tcctcgttcg gtttaggtgg aaggcgaggc ggctgaagaa taccatttcc
     2401 agaaaagtgc agggtgatcg tcgagagaat tcacgatcaa tgtgatcgcc ttctcacctg
     2461 ctgtgcaccg tctactcttg ggctgtaccg aactgctcgt tcaggatagg tgatggcgcg
     2521 gcttcactca gcagctcacg cgtcgtcggg gccgtgcggt gatgggtggc gtagagcgca
     2581 agcccggtga acacgaatcc
//
LOCUS       DATMXI010000360         5320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_295054, whole genome shotgun sequence.
ACCESSION   DATMXI010000360 DATMXI010000000
VERSION     DATMXI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295054"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VN444_06960"
     CDS             complement(<1..1157)
                     /locus_tag="VN444_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWR21582.1"
                     /translation="MPRGTQKHRGFDPDEAGCLRLIEALADGNPIPDTSAICPDRQRL
                     IALLAEISKGERSELFQSVRQVAERRGLSSQEIASKAAFLLACLDTPGADDPYTILGI
                     DPTATSEEIKEAWLSRLSLYHPDRHPENSDWFTRQAARLNEAYQRLKDPARRQAYDER
                     RRRELLAQQQSGPFAIQAVSSAPPPMPSQFSRLARHRAPALIMTASVAAAGLALMALS
                     GRPPAGPQLYIETLQPVAAGTLPSPTSTGGPQPAGPIRGTPPFLEAAATSPIDRQPER
                     RRMKRVLQASLPSGATDAIDATTQQTQLLLAQALPPIVPEPKGLDRQEIDALLDEYID
                     AYEKADVERVMATLSTRVREKGTLDYQTIRNAYIKGFTGRDQIIYRLKNLQV"
     gene            complement(1229..2389)
                     /locus_tag="VN444_06965"
     CDS             complement(1229..2389)
                     /locus_tag="VN444_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21583.1"
                     /translation="MEPNESAIRHGLAIDPDNDRIHAIRATLFHYSLLLRDYPAALTA
                     YRSALRHNPLNSASWLHLGKLYATLGQARESGQAFALAARFGPSHSALLWEIAIAHLD
                     EGQVTEAMTTLTKFLAVSPPNDASRGYELAHRLASPDAVIEKMIAPDADHYTHYANYL
                     LDRNLGDQALAVWNRLAELAARTREPIDPHLQLRVIDLLMATDRLDQAYQLWTNLTTQ
                     MKLGAAPTAANLMSNGNFEHKETIGRGFDWRIGGAPGVAWTFDPDTAHTGRRSLRLSF
                     TKSRADFSNVSQLIPVQPHSMYALQAHIKTDGLAGSRGITVEVIDSAEGALAKTDAIG
                     GTRNWSPVTVKFRTSATARTITLKVHSEPPPPYLPPLSGSAWIDNVSLTKVE"
     gene            complement(2534..4912)
                     /locus_tag="VN444_06970"
     CDS             complement(2534..4912)
                     /locus_tag="VN444_06970"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWR21584.1"
                     /translation="MDALVKADRNSPSPREQLLQRYYLQQDEETHLRDYWRIILKHRW
                     IVLTFFAIIVTTAMIYTFSLTPTYRATASLKIDYERPQILSFQEIGSPGHQNYGPEFM
                     GTQQKLLQSRSLAKRVIETLKASGQPILLDQALPGPVATTAFWPVWLTDLFGSRSSKP
                     TEATGPALRSESETGLTEEQLSSRKVDVLLRMLTVEPIRASNLVKISFTTPSSDLSSL
                     LANTWVKAFIDHNFDMKYNATAQAGEWLSKQLQDVRDKLEQSEAALHEFVKQKQILTT
                     GEKKDIVTTKLGDLSEALTKAQGERIAREALYRQSQKMGFDSIPAVLENPMISNLRAE
                     YYKLDAESRRLSETYKPGYPKMVRLRESMNQIKRQIETEITRIVDAIRQEYEAAVKKE
                     QLLQAALNEQKHVAIALNQELIGYDILKRDVDTNRQIYSGILERQKQAGVSQGLAASN
                     VQIVDLAEPPSAPFSPNKTRNILLGIILGLTVAIGMSFFFDYLDNTVKHSEQLERTLG
                     IPSLGLIPSLASASPRQGRGGLSRDGGNGNHNGSKPAFALIAHSNLRSTLTEAFRTLR
                     TSLLFSSAGSPPKSILFTSAQPSEGKTGLSINTAISLSQLGGSVLLIDGDMRRPSCHS
                     HLELPLKPGLSDYLTGNADLVSIIKRTTVPNLHCIPAGTISVNPAELLASPRTQETIE
                     LLSQRFDYIVVDSPPIFAVADALILSTVVKGVILVVQGGRTSREMVQRAFKNLIELNA
                     PVLGAVLNNVNIRGNGYPYYYHHHYSHYYQQTPADAPHPPDAEKAQVDEMTG"
     gene            complement(4982..>5320)
                     /locus_tag="VN444_06975"
     CDS             complement(4982..>5320)
                     /locus_tag="VN444_06975"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HWR21585.1"
                     /translation="KSGEFLLGGAVKKPGPYPLKGKLTVDQAIYFGEALKEDADLTDI
                     EVLRINGSKNEVFKINLNQLKQDGTPGLAIQKNDVVFVGTSGPKAVMYGALDFFKTII
                     RFGVSAGAAL"
BASE COUNT         1045 a         1438 c         1661 g         1176 t
ORIGIN      
        1 acctgcaggt tcttgagccg gtagatgatc tggtctcgcc cggtaaaccc ttttatgtag
       61 gcgttgcgga tggtctgata atccagggtc cccttctccc gaacccgggt cgagagggtg
      121 gccatcacgc gttccacgtc agctttttca taggcgtcaa tatactcgtc gagcagggcg
      181 tcgatctcct gccgatcgag gcctttcggc tcaggcacaa tgggcggaag ggcctgggcc
      241 agtagtagtt gcgtctgttg cgtcgttgcg tctatagcgt ctgttgcgcc agaaggcagg
      301 gaggcttgaa gcacacgttt catgcgccta cgctccggtt ggcgatctat gggagaagtg
      361 gcggctgctt cgagaaaagg gggagtgcct cgaattggtc cagcgggttg agggccacca
      421 gtggatgtag gggacggaag ggtacccgcg gccacgggct gaagcgtctc gatatagagc
      481 tgcggtccgg ccggagggcg accggacagc gccatcagag ctaatccggc cgctgccacc
      541 gatgcggtca taatgagtgc cggcgctcga tgacgcgcca ggcgcgaaaa ctgagacggc
      601 atgggaggcg gtgcggacga aacggcctgg atcgcgaagg ggccgctttg ttgctgcgca
      661 agtagctcgc ggcgtcttcg ctcatcgtac gcctgccgtc gcgcagggtc tttcagcctt
      721 tggtacgctt catttagacg cgctgcttgc cgcgtaaacc agtcactatt ttccgggtga
      781 cggtcgggat gatacaggct caggcgactg agccaggcct ctttgatctc ctcactcgtt
      841 gcggtcggat cgatcccaag aatggtatac gggtcatcgg cgcccggggt atccaggcag
      901 gcgaggagga aggccgcctt ggacgcgatc tcttgcgagg agagccctcg acgctccgcc
      961 acctgccgga cgctctggaa aagctccgat cgctcccctt tgctgatctc tgcgagaagg
     1021 gcgatgagtc gctgcctgtc cggacagatg gcagatgtgt ctggaatcgg attaccatcg
     1081 gctaacgcct cgatcaaccg aaggcacccc gcctcatccg gatcaaagcc cctatgcttc
     1141 tgcgtccccc gtggcattct taacccttaa cgttgaacct tgaacattga acgtcgcacc
     1201 tcgaacgttg aacgttgaac tatgaacttt actctacctt cgttaaggag acattgtcaa
     1261 tccatgccga cccggagagc ggggggagat agggaggggg aggctcgcta tggactttga
     1321 gcgtgatggt ccgggcagtg gctgatgttc ggaatttcac cgtaacggga ctccagtttc
     1381 gtgtcccccc gattgcatcg gttttagcca gggccccctc agcggaatcg atcacctcga
     1441 cagtgatccc cctggacccg gcgaggccat cagttttgat gtgggcctgg agggcgtaca
     1501 tggaatgagg ctgaaccggg atgagttgcg agacgttcga gaaatcagcg cggctttttg
     1561 taaaggagag tctgagggat cggcgtcctg tgtgggccgt gtcagggtca aaggtccagg
     1621 caactcccgg tgcgcctccg atcctccagt cgaaccccct gccgattgtc tctttgtgct
     1681 caaaattacc gttggacata aggttcgctg cggtgggggc cgcgccaagc ttcatctgcg
     1741 tcgtgaggtt ggtccacaac tggtacgcct ggtcaagcct gtccgtcgcc atcagcaggt
     1801 cgatgacccg gagctgaagg tgtgggtcta tcggctcacg ggtgcgagcg gccagttctg
     1861 cgagccggtt ccacactgca agagcctgat cgccgaggtt gcgatcgagc aggtaattcg
     1921 cgtagtgggt atagtgatcg gcatcggggg cgatcatctt ctcgatcacc gcgtccggcg
     1981 aggcgagtct gtgtgccagc tcgtatcccc gtgacgcgtc attggggggt gagacggcca
     2041 gaaacttggt caatgtggtc atcgcctcag taacctgccc ctcatcaaga tgggcaatcg
     2101 caatctccca taacaaggct gaatggcttg ggccgaatcg ggcggccagg gcgaacgcct
     2161 gaccggactc gcgtgcctgg cctagagttg catagagctt cccaaggtgc agccaggagg
     2221 cgctattgag tggattgtgt cgaagggctg accgataggc ggtgagcgcc gcagggtaat
     2281 ctcgaaggag caggctgtaa tgaaacaggg tagcacgtat ggcatgaatg cggtcgttat
     2341 cgggatcaat cgccaggcca tgccgaatgg cgctctcatt cggctccacg cccgtaactc
     2401 gttgagcgat ccatggacgg agcgcagccc atgaggcaag tgcaaaacca aggaccagga
     2461 tacctgagaa cgcgagtcgc gatggccgac cgtgcaggtg aagtaccatt ttagtctaga
     2521 tggtctcttt ggtctatccg gtcatttcgt caacctgagc cttctcggcg tcaggagggt
     2581 ggggcgcatc ggcgggcgtc tgctggtagt aatgactgta atggtggtgg tagtagtacg
     2641 gatagccgtt gcctcggata ttgacattat tcaggacggc gcccaggacc ggcgcattga
     2701 gctcgatgag gtttttgaag gcgcgctgaa ccatctcgcg tgacgtccgc cccccctgga
     2761 ccaccaggat gacgccctta accactgttg agaggatcag cgcatcggcg accgcgaaga
     2821 tcgggggcga gtctacaacg atgtagtcga accgttgaga gaggagttca atcgtttcct
     2881 gcgtcctcgg agaggccaac agctccgcgg gattcacgga gattgttcct gcggggatgc
     2941 agtgcagatt cggcactgtg gtccgcttga tgatcgagac cagatccgca ttgccggtca
     3001 gataatccga gagaccaggc ttgaggggaa gctccaaatg ggaatgacag ctcggtcgcc
     3061 gcatatcgcc atcgatgagg agcacgctac cccctaactg agagaggctg atggcggtgt
     3121 ttatggatag tccggtcttg ccttccgagg gctgggcgct ggtgaacaga atcgacttcg
     3181 gaggcgaccc ggcggaggag aagagtagcg aggtccggag cgttcgaaac gcctctgtca
     3241 gcgtggagcg cagattactg tgcgcgatca gcgcaaaggc cggtttactc ccattgtgat
     3301 tcccgttgcc gccgtctcgc gagagcccgc ctcgcccttg ccgagggctc gcggaagcca
     3361 gcgaggggat cagacccagg ctcggaatgc caagggtgcg ctcgagttgc tcagagtgct
     3421 tcacggtgtt gtccaggtag tcaaagaaaa aggacatgcc gatagccaca gtcaacccca
     3481 gaataatgcc aagtaagata ttccgagtct tgttcggact gaacggggcg ctcgggggtt
     3541 cggccaggtc cacgatctgg acattactcg ctgcaagccc ctgggagacc cccgcctgct
     3601 tttggcgctc aaggatgccg ctataaattt ggcgattggt gtcgacatcg cgcttcagaa
     3661 tgtcgtaccc gatgagttcc tggttcaagg cgatggcgac atgcttctgt tcgttgaggg
     3721 ccgcctggag gagttgctcc ttcttgaccg cagcctcata ctcctgcctg atggcgtcaa
     3781 cgattcgagt aatctctgtc tcgatctgac gtttgatctg gttcatactt tcgcgcagac
     3841 ggaccatctt ggggtacccg ggtttatagg tctcgctcag tcggcgagac tcggcgtcta
     3901 acttgtagta ctcggccctc aagttggaga tcatggggtt ttccaggaca gccgggattg
     3961 agtcgaatcc catcttttgg ctctggcggt acagcgcctc cctggcgatc cgttccccct
     4021 gggcctttgt cagtgcctcg ctcaaatcgc caagttttgt cgtgacgata tctttcttct
     4081 ctcccgtggt aaggatctgt ttctgcttca cgaactcgtg tagagcggcc tccgattgct
     4141 cgagcttgtc ccgcacgtct tgaagctgct tcgaaagcca ctcccccgcc tgggcggtag
     4201 cgttgtattt catatcgaaa ttatgatcga tgaaggcctt gacccaggta ttggccagga
     4261 gggaggagag atctgacgag ggggtagtga aggagatctt gaccaggttc gatgcccgga
     4321 tcggttccac cgtcagcata cggagcagca catcgacctt gcgggaactc aactgctcct
     4381 cagtgaggcc tgtctcagac tctgatcgga gagcgggccc ggtcgcctct gtaggcttcg
     4441 aggaacggga accgaaaaga tcggtcagcc atacgggcca gaaggctgtc gtggccacag
     4501 gaccgggcag tgcctgatcc agtaggatcg gctgaccgct tgccttgagc gtctcaatca
     4561 cccgcttcgc caaactgcgg ctctgcagca gtttctgctg ggtgcccatg aattcggggc
     4621 cgtagttctg gtgtccgggg gacccgatct cttggaagga aaggatctgg ggccgttcat
     4681 aatcgatctt gaggctggca gtggctcgat aggtgggggt caggctaaag gtatagatca
     4741 tggcggtggt tacgataatg gcgaagaagg tgaggactat ccagcggtgc ttcaggatga
     4801 ttcgccagta atcgcgaagg tgcgtctctt cgtcttgctg gaggtaatag cgctgcagca
     4861 gttgctcacg cggacttgga gaattcctgt ccgccttaac taacgcatcc atacggcaga
     4921 accgcctcct tgagcgtcca tagcgtcttt agcgtgtgac gcaataaacg tactagacgc
     4981 actagagggc cgcccctgct gagacgccga aacggatgat cgttttgaag aagtcgaggg
     5041 cgccatacat gactgccttc ggcccgcttg tgcccacgaa gacgacatcg tttttctgga
     5101 tggctagtcc aggcgtaccg tcctgtttca actggttcag gttgatcttg aacacctcgt
     5161 tctttgaccc gttgatacgt aagacctcaa tgtcggtcaa gtcggcatct tctttaagcg
     5221 cctcgccaaa atagattgcc tgatcgacgg tgagtttccc tttgagaggg tagggaccag
     5281 gcttcttgac ggccccaccc aggaggaatt ctccggactt
//
LOCUS       DATMXI010000361         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_225663, whole genome shotgun sequence.
ACCESSION   DATMXI010000361 DATMXI010000000
VERSION     DATMXI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225663"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VN444_06980"
     CDS             complement(<1..1246)
                     /locus_tag="VN444_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWR21586.1"
                     /translation="MRQLVSVALLAIAVAVVGCGKEDRAPSPGKTETGRVAHLDGPEG
                     HPHDQASHNGEHRETVGSSEDSIGVRLTSEERENIGLRTEIVQLQSIEDVRKLNGIVK
                     PHPDRVAQVTSRVPGIVLSVHASLGAWVRRGEDLLDIKSVELERLELSLIQAENRLAL
                     IKLDLERVQTLVERGILARKELLALENQHREVLNEIESLTRQLIFLGLARGAIARIRE
                     EQMIATLHLPAPISGTIVERNVVLGQAIESNAALMKIIDTSTMIVEGEAFEDILPMLQ
                     LGQKVRVVVSAYPNEVFAGRISFISPTVNPTKRTIPVWAEVGNPRGLLKQDLFAQVYV
                     IVGEQRRSLTIPIEALISAEGAEFAFVERDGVYVRADLGLGTRNDRFAEVKRGLASGD
                     RVITDGSRQLYAKWLAAKGGGPA"
     gene            complement(1445..1831)
                     /locus_tag="VN444_06985"
     CDS             complement(1445..1831)
                     /locus_tag="VN444_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21587.1"
                     /translation="MGRGSGSIARTVALIAAISLLWVYPFCFFHQASAVAYQHVTSSD
                     LDHRSSPPVACDDSHLYAVSSGQVTSAGAKAGISLRIEPLRAIPCERFLAMRRHELTG
                     PLLPASRSASLSASKALYVLYVVYQI"
     gene            complement(2186..>2825)
                     /locus_tag="VN444_06990"
     CDS             complement(2186..>2825)
                     /locus_tag="VN444_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWR21588.1"
                     /translation="EGWGCDLVGMCVGHVPLDRIIVGKSVAPGDVIIGIRSSGIHSNG
                     LTLARRVLFAQAKCTVNTKFEELGRTLGEELLEPTIIYVPEVVEALERPLAIKGLAHI
                     TSDGLLNLLRLDTASHPVGYEIDQLPPIPPIFSLIQQSGQIPDHEMFQVFNMGIGFCV
                     VIAEEDAESFVEIVKHHGREAMQIGRVVSDSERKVVIRSRGLVGQGNQFVKV"
BASE COUNT          632 a          868 c          741 g          584 t
ORIGIN      
        1 gtgccggtcc gccgcccttt gcggcgagcc acttcgcgta gagctggcgg ctgccgtccg
       61 tgataacccg atcgccagaa gccagacctc gcttgacctc ggcgaagcga tcattccggg
      121 ttccaagtcc aaggtctgca cggacataca cgccgtcccg ttcgacgaag gcgaactccg
      181 ccccctccgc gctgatgagc gcctcaatcg ggatcgtgag gctcctgcgc tgctctccca
      241 cgatcacgta gacctgggca aagagatcct gcttgagcaa cccgcgggga tttccgacct
      301 ctgcccacac cggaatggtc cgcttcgtcg ggttgaccgt cgggctgata aagctgatcc
      361 ttcccgcaaa cacctcattg ggataggccg agacaacgac ccggaccttt tgcccaagct
      421 gaagcatcgg caggatgtcc tcaaaggcct ccccctcgac gatcatggtg gaggtgtcga
      481 taatcttcat caacgcggcg ttcgactcga tcgcctggcc gagcacaacg ttccgctcca
      541 cgatcgtccc actgatgggg gccggtagat gaagcgtggc gatcatctgt tcttcacgaa
      601 tccgtgcgat cgccccacga gccaggccga gaaagatcag ttggcgcgtg agactctcga
      661 tctcattcag gacctcccga tgctgatttt cgagagccag caactccttt cgggccaaaa
      721 tccctcgttc aacgagtgtt tgcactcgct ccagatccag ttttattagt gccagcctat
      781 tctctgcctg aatcaggctc agttccaacc gttccagttc gacgctctta atgtctaaca
      841 gatcttctcc cctcctgacc caagcgccaa gcgacgcatg aacactgagc acgatcccgg
      901 gcacgcggct ggtcacctgc gccactcgat caggatgcgg cttgacaatc ccgttcagct
      961 tgcggacatc ctcaattgat tgaagctgta cgatctcggt cctgaggccg atattctcgc
     1021 gctcttcgga agtcagtcgg acaccaattg aatcctcgct agaaccgacg gtctccctat
     1081 gctctccgtt atgggacgcc tggtcgtgcg gatgcccctc cggaccgtca agatgtgcga
     1141 cccggcctgt ctcggtcttg ccgggcgagg gcgcacgatc ctccttgccg cacccgacta
     1201 cagcgacggc gatcgccagc aatgccacgc tcaccagttg cctcacctct cgcatgtttc
     1261 cttacactcc cgctgcccaa gcaggacatc cgttaattgg aattgttcaa ccatagaccc
     1321 ctcgccgtcg atgaggtgaa aagaacggaa agccggatct ccttgtcggg tggatcgcac
     1381 tagtagaaag cggaatctgt acggtcgagg agatacctac aacacgagaa tccgggcggg
     1441 gcaattaaat ctgatagacc acgtacagca cgtacaacgc tttcgaagcg gaaagcgagg
     1501 cggatcgaga agcggggagt aaggggccgg tcagttcatg tcgcctcata gccaagaacc
     1561 gttcacatgg aattgctcgc aaaggctcga ttcggaggct gatgccggcc tttgcgccgg
     1621 cgctcgtcac ttggccgctt gagactgcat agagatggga atcatcgcag gctacaggag
     1681 gggatgaacg atgatcgagg tcggacgagg taacgtgttg gtaggcaaca gcacttgcct
     1741 ggtggaaaaa gcaaaacgga taaacccaca acaggctgat agccgcgatg agagccacgg
     1801 tccgagctat cgatccagat ccccgtccca ttgagtcatc cttttcgccc cccgatttct
     1861 cgggggcagg tatcgaggac aacttaatgc taccacgacc tacatatgct ttcaattcag
     1921 aaagctcaaa gtcgtgctgg gcactggcaa actctcagtc ccatggcaga agaagaccgt
     1981 tagtaactac tcaggtagat agactgtagt cttttagact ataggaacat gcacagatga
     2041 gccaggagtt ttctgaatgc gacgcgaaca tgttggaggc cgaaaaggtt ctgggtgcct
     2101 tgattcggtc catgcgcaac ggctaacagc cttcagccta cagcctaaac acctgagtag
     2161 ttacgaccgt tcctcacatc gttgactaca ccttcacaaa ttgattgccc tggccgacca
     2221 gaccgcgtga tcgaataacg acctttcgct ccgaatcact gaccacacgg ccgatctgca
     2281 tggcttcccg gccatgatgc ttcacgatct ccacaaagga ctccgcgtct tcctcagcaa
     2341 tgacgacaca gaacccgatc cccatattga agacctgaaa catctcatga tcggggatct
     2401 gcccggattg ctggatcagg gagaagatcg gagggatcgg cgggagttga tcgatctcat
     2461 acccgaccgg atgagatgcg gtgtccagtc gcagaaggtt cagcaaccca tcgctcgtga
     2521 tatgggcgag ccctttgatg gccagcggtc gctcaagcgc ttcaacgact tccggcacat
     2581 agatgatggt cggttccagc aactcttccc ccagtgttcg cccaagctcc tcgaacttgg
     2641 tattcacagt gcacttggcc tgcgcgaaca ggacccgccg ggccagcgtt aggccattac
     2701 tatggatccc gctgcttcgg attccaatga tgacatcacc gggagccacc gactttccaa
     2761 cgattatcct gtccagcggg acatggccga cacacatccc gaccagatca cagccccacc
     2821 cctcc
//
LOCUS       DATMXI010000362         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_281192, whole genome shotgun sequence.
ACCESSION   DATMXI010000362 DATMXI010000000
VERSION     DATMXI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281192"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VN444_06995"
     CDS             <1..1022
                     /locus_tag="VN444_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR21589.1"
                     /translation="LVGFRDRCLKPVSLRPEPLSTVDAARVNAWRAFFASPAAPVAPA
                     VSAEVGVFISPLNAAMPRVFETDWLAARLGTPGLKVIDLRPQPEYNAAHIPGSLSLNV
                     ESFRGNIGGVPSVLLPPPMLAAQFSLLGLQPSDTIVLVSGDKLYDTTLAGMAFERLGH
                     MDYAVLNGGYAKWAQENRPTDAALPTVTESKYPVKKDADDFTVDFQNVAAQLGKPGVV
                     IIDVRPADYYTGKKSDEARAGHIPGAVSRPFTEDVITSSTKVVSFKPADALAAAYSQI
                     IPSKDTEVIVHCRTGHQASQTFFVLKRLLGYTNVRWYDAGWTEWAARSELPIVNETKD
                     VKSKR"
     gene            complement(1115..1777)
                     /gene="phoU"
                     /locus_tag="VN444_07000"
     CDS             complement(1115..1777)
                     /gene="phoU"
                     /locus_tag="VN444_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWR21590.1"
                     /translation="MARKEFDQELHDLEKRLQELGLMVEEALVEAVDALKRRDLEKAG
                     QIITADRSVNERRYAIEHETFVLIATQQPMAVDLRTLAAVLEIATELERIGDYAKGIA
                     KITIKMGTEPFVKPLIDIPRMAEKARNMLQRALQAFAGRDTDLARAIPAEDDEMDALY
                     EQIFRELSTHIMVDPKLVDRASYLIWVAHNLERAADRVVNICERVIFMVTGEVVEFDD
                     NL"
     gene            complement(1849..>2294)
                     /locus_tag="VN444_07005"
     CDS             complement(1849..>2294)
                     /locus_tag="VN444_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21591.1"
                     /translation="VAFGPRVLGLGNRSELDRIVEDSLHGGVLWDEVKDKLHQDALSL
                     SLGQQQRLCIARTIAVKPDVILMDEPCSALDPIATLHIEELMRVLKANYTIVIVTHNM
                     QQAARVSDVTGFFWMGELVEFDVTKSIFTRPSQKLTEDYITGRAG"
BASE COUNT          464 a          707 c          630 g          493 t
ORIGIN      
        1 gcctagtggg tttccgggat cgttgcctca agcccgtctc gttacggcct gaaccgctgt
       61 caacggtcga tgccgcccgg gtcaacgcct ggcgcgcctt tttcgcctcg ccagctgcac
      121 ccgttgcgcc agccgtttcc gccgaggtcg gcgtttttat ctcgccgcta aacgccgcca
      181 tgccgcgcgt cttcgagacg gactggctgg cggcccggct cggcacgcca ggtctcaagg
      241 tcatagatct gcgaccccag ccggaataca acgcggcgca cattccgggt tccctcagcc
      301 tcaatgtcga gagtttccgc ggcaacatcg ggggagtgcc gtctgtgctc ctgccacccc
      361 ccatgctcgc cgcgcagttt tcgctcctgg ggttgcagcc ctcagacacc atcgttctcg
      421 tgtccggcga caagttgtac gataccacct tggccggcat ggcgttcgag cggctggggc
      481 acatggatta cgccgtcctg aacggagggt acgccaagtg ggcgcaggag aaccggccca
      541 cggacgccgc gttacccacc gtaaccgaat cgaaataccc ggtgaaaaag gacgccgatg
      601 acttcacagt ggatttccag aatgtggccg cccaactcgg caagccgggc gtcgtgatta
      661 ttgacgtgcg gccggcggac tattacacgg gcaaaaaatc cgacgaagcc cgtgccggtc
      721 atatccccgg cgcggtcagt cgccctttca ccgaggatgt catcacgagc tccaccaagg
      781 tcgtctcctt caagccggcc gacgccctgg ccgcagcata cagtcagata attccctcca
      841 aagatacgga agtcatcgtg cattgccgca ccgggcatca ggccagccag accttcttcg
      901 tgctgaagcg tctgctgggc tatacgaatg tccgatggta cgacgccggc tggaccgaat
      961 gggccgctcg ttcggaactt ccgatcgtga acgaaacgaa ggacgtcaag agcaagaggt
     1021 gaagcttacg ggatgtgagc cgctcggaca taactctaac tgaaaaactg ctactaaaac
     1081 tgggggcagg tcagaagcag ccgctgctca gatcctagag gttgtcgtca aactctacga
     1141 cttcaccggt gaccatgaag atcactcgct cgcaaatgtt caccacgcga tcggcggccc
     1201 tctccaggtt atgggccacc cagattagat aactggctcg atcaaccagt ttgggatcga
     1261 ccatgatatg ggtgctcaac tcgcggaaga tctgttcgta gagagcgtcc atttcatcat
     1321 cttcggcagg aatcgctcgg gccaggtcgg tatcccgtcc agcgaaggcc tgaagcgctc
     1381 gctgcagcat gttgcgtgct ttctcggcca tgcgtgggat atcgatcaga ggcttgacaa
     1441 agggttcagt ccccatcttg atggtgattt tggcgatccc cttggcgtaa tccccaatcc
     1501 gttccaactc ggtggcgatc tccaggaccg cggcgagagt cctcaaatcc accgccatgg
     1561 gttgctgtgt ggcgatgagc acaaaggttt catgttcaat ggcataacgc ctctcattca
     1621 ctgaacgatc ggcagtgatg atttgccccg ccttttccag gtcacggcgt ttcaaggcat
     1681 ccactgcctc caccagtgct tcttccacca tcaggcccaa ctcctggagt cttttttcca
     1741 ggtcgtgcaa ttcctggtca aattcttttc ttgccatagc ctgattggta cctccatcgg
     1801 caaaaaggtg ctctgtccgg tcccggtcta ttccaggccg tcacgcgctc agccggcccg
     1861 cccggtaatg tagtcttccg tcagtttctg gctgggccga gtgaagatcg acttggtcac
     1921 atcaaattcc accaactcac ccatccagaa aaaaccggta acgtccgaca ctcgcgcggc
     1981 ttgttgcatg ttgtgagtga cgatgacgat ggtgtagttg gctttgagca ctcgcatcaa
     2041 ttcttcgata tgcagtgtgg cgatgggatc aagagccgaa caaggctcgt ccatgaggat
     2101 gacgtcgggc ttgacagcaa tggtccgggc aatgcagagc ctctgctgtt gccccagcga
     2161 aagactgagc gcatcctgat ggagtttgtc cttcacttca tcccagagca ctccgccgtg
     2221 aaggctatct tccacaatcc ggtcaagttc actgcggttc ccaagcccca gcacccgcgg
     2281 gccaaaggcc acgt
//
LOCUS       DATMXI010000363         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_119877, whole genome shotgun sequence.
ACCESSION   DATMXI010000363 DATMXI010000000
VERSION     DATMXI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119877"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1280)
                     /gene="mnmA"
                     /locus_tag="VN444_07010"
     CDS             complement(198..1280)
                     /gene="mnmA"
                     /locus_tag="VN444_07010"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWR21592.1"
                     /translation="MARPTVLLGMSGGVDSSVAAALLVRQGYDVHGVTLQVWEHEDET
                     VAASKRWEERGCCKVGIARFVAQTLKIPYEVVDTRATFRAGVIDNFVAGYLEGTTPNP
                     CVRCNERVKLRGLIELANTRGIKFVATGHYVRIGESRGMRTLQRATDLKKDQSYFLYR
                     LRHAWLERLLFPVGDMEKADVWKEAGALGLPVEELKESQEICFVSHGDYRTFIEKESP
                     SAKNPGAFVDDAGQYLGEHDGIAFYTPGQRRGLGIATGRRLYVQQVLPATNTVVLGAE
                     ESLRRSHCDITDLNLFDPSLLTGPTEADIKVRYATAATPATIQPLSEGTLRISFHVPQ
                     RALSPGQSAVFYRNDHVLGGGIIQSF"
     gene            complement(1336..1761)
                     /locus_tag="VN444_07015"
     CDS             complement(1336..1761)
                     /locus_tag="VN444_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWR21593.1"
                     /translation="MWPTRERVQQHEQNSENLTLLGKGAEFRGVVSFDGTLRVDGRVE
                     GEIYTTGTLIVGEHAVIEGIVSAGVLMNSGKINGTITASEKILILKPGVLVGDIRTPV
                     IAIEEGSHFHGMCDMGAHHWSNDRALALDISPSFVAAAH"
     gene            complement(1850..>2116)
                     /locus_tag="VN444_07020"
     CDS             complement(1850..>2116)
                     /locus_tag="VN444_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWR21594.1"
                     /translation="AVIQAKITAGTVVCKGKITGDVLAKECVKLRAPAVMNGSVKTPV
                     LSMEDGVLFNGALEMAHGVRELPREVSLHPVGMIMPPGIKRVNG"
BASE COUNT          461 a          580 c          593 g          482 t
ORIGIN      
        1 gaatttgaga ccgttagccc gcattattca tgactgttcg tggcgaaatc gccacgaaca
       61 gtcatgagac cggcgcgcgg agcccggagg atctgaggtg tactcgtgca gtacgtcgaa
      121 ggtctgaggg gcgagcctgc cggcttgttg caacagcgga tcggcgattg cagcagaaga
      181 gtcctgaata atgcgggtta gaatgattgg atgataccgc cgcccagtac atgatcgttt
      241 cgatagaaga ccgctgactg ccctgggctc aacgctcgct ggggtacatg aaaggaaata
      301 cgcagggtgc cctctgaaag gggttggatg gttgcgggcg tggcagctgt agcataccgg
      361 actttaatgt cagcctctgt tggtccagtg aggagggagg ggtcgaagag attgagatcc
      421 gtaatgtcac agtggctgcg acgtagtgat tcttctgctc caagcacgac ggtattggtg
      481 gccggcagta cttgctgtac atagagcctg cggccagtgg caataccgag acctctccgc
      541 tggcctggcg tgtagaaggc gataccgtca tgttcgccca aatattggcc cgcatcgtcg
      601 acaaaggccc ctgggttctt tgcggagggc gattcctttt cgatgaaggt ccgataatcg
      661 ccgtggctca caaaacagat ttcctgactt tccttcaatt cttcgacagg aagacccaag
      721 gcccctgctt ccttccatac gtccgccttc tccatgtctc ccacaggaaa aagtaggcgt
      781 tcgagccacg cgtggcggag acgatagagg aagtagcttt ggtctttctt aagatccgtt
      841 gctcgctgaa gcgtccgcat tccccgggat tcgccaatgc gaacatagtg gccagtggcc
      901 acaaatttga ttccgcgtgt gttcgcaagc tcgatgagac cacgcagctt gacccgctcg
      961 ttgcacctga cacaggggtt gggtgttgtc ccttcgagat agccggccac aaagttgtcg
     1021 atgacgccgg ctcgaaaggt tgctctggtg tcgacgacct cataaggaat tttcaatgtc
     1081 tgtgcgacaa accgggcaat accgaccttg cagcagccgc gttcctccca ccgcttagag
     1141 gccgcgacgg tttcatcttc gtgttcccag acctggagcg tgacgccgtg gacgtcgtac
     1201 ccctggcgaa cgaggagcgc tgccgcgacg gagctgtcca ctccaccgct catgccgaga
     1261 agaactgtag ggcgagccat acagtcgcta ttctactggg aagacgggaa aagggctaga
     1321 gggcgcgtcc tctgactaat gggcggccgc gacgaagctg ggtgagatat cgagcgcgag
     1381 agcgcggtcg ttgctccaat gatgcgcacc catatcacac atgccatgga aatgagagcc
     1441 ttcttcgatt gcaataactg gggtccggat atcaccgaca agaacaccgg gtttaaggat
     1501 gagaattttt tcactagcag tgatagtgcc attgatcttg ccgctattca tgaggactcc
     1561 ggctgacacg atgccctcga tcactgcatg ctcaccgacg atgagtgtcc cagttgtata
     1621 gatctcgcct tcgactcgtc catcaacccg gagggtgccg tcaaaactga cgacccccct
     1681 gaactccgcg ccttttccca aaagggtaag gttttcactg ttctgctcat gttgttgaac
     1741 gcgttctctt gtcggccaca tgattgcctc ctacagtcct ctcccatgcc tgcacagtgt
     1801 tcgccagaca agtaaagagc tgaaaaatac ctacatatta atatttcccc tagccattga
     1861 ctcgtttgat gccagggggc atgatcattc ctacggggtg cagagagact tccctgggga
     1921 gttcgcgcac gccgtgagcc atttcaaggg cgccgttaaa tagtactcca tcttccatag
     1981 aaaggaccgg cgtcttgacg ctcccattca tcaccgctgg ggcgcggagc ttcacgcact
     2041 ccttggcaag gacatctccg gtgattttac ccttacacac aacggtaccg gcagtaattt
     2101 tcgcctggat gacggc
//
LOCUS       DATMXI010000364         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_139373, whole genome shotgun sequence.
ACCESSION   DATMXI010000364 DATMXI010000000
VERSION     DATMXI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139373"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..922
                     /locus_tag="VN444_07025"
     CDS             314..922
                     /locus_tag="VN444_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21595.1"
                     /translation="MSIRGYASPESVYVERGRISLNAWRLEGIATLVLLMLTACASSY
                     VADGFFVDGTRGFKIPVLRDGWRQFELEGTDLAFRAEPGGQVAALFVSCEGEQPIPLR
                     ILARRLFFGIGTKRIIAQEPIVLNGTEAIHTLMEGRLNDTEVMVSSYVAKNGDCAYDL
                     VYVAPPELFQDRLPEFERFARGWVLAEKGSRFQVPGSTLRLR"
     gene            945..1730
                     /locus_tag="VN444_07030"
     CDS             945..1730
                     /locus_tag="VN444_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR21596.1"
                     /translation="MRKVAVAVGGQSIRLLELLGGLSKLTYDTVYCAFTPPYNPRGLL
                     QQIDHIGVKSISIASVAAVFTGLVLALQTAYGLSRFGAKAYVGIIVSLSMVRELGPVL
                     TALLVGGRVGSGITAELGSMKVTEQIDAMRAMGANPIKKLVVPRVLSAMLVLPLLTVM
                     ADVLGMLGGMVISRYEFQVDYHLYYNTVTRNLTVADIVSGLGKTVVFGFIIAIVGCYR
                     GLETVGGTEGIGRATTAAVVTSAIAVIISDFFLTKFFWWLEGW"
     gene            1732..>2016
                     /locus_tag="VN444_07035"
     CDS             1732..>2016
                     /locus_tag="VN444_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR21597.1"
                     /translation="MSAPAIEFRQVYKSFNHTPVLAGMDFTIQSGETVTIIGGSGIGK
                     SVTLKLIVGLLKPEGGRILIEGEDIVPLAEDQLIRVRKKIGMVFQGSALFD"
BASE COUNT          387 a          545 c          641 g          443 t
ORIGIN      
        1 cttacatggc atccgtcggc aatactgggt gctgtgcgag gtgacctgag ggcgggcgag
       61 gacaggaaga ggcgcaccca ggacgaaggg gggagtagtc tgcctcggct cgggtgatcc
      121 ggtcctcgat ggaccgcgag agcaggataa cccctgcaaa gatcctcgcc cctcgccgat
      181 tctactggtc acagggtcgt acaaccgccc tgatcagcac acgtttgcat ctcagcaatg
      241 tctgtctcta gttttttggc acagggctca gcttgacgct tcgctcgtct ttccagtatg
      301 atgaagtaac tatatgtcta tccgcggtta tgcctcacct gaaagtgtat acgtcgaaag
      361 ggggcgtatc tccctgaatg cctggcgcct tgagggcatt gcaaccctcg tcctgctcat
      421 gctcacggcc tgtgcttcca gttatgtcgc agatggcttc tttgtggatg ggacccgagg
      481 cttcaagatt ccggtgctac gcgatggttg gcggcagttc gagttggaag gaactgacct
      541 cgcgttcagg gcggagccgg gtggccaggt cgccgcattg tttgtcagtt gcgaaggcga
      601 gcagcccatc cctctccgaa tcctggccag acgcctgttc tttgggatcg gcacaaagcg
      661 gataatagcg caggagccca ttgtgctgaa cggcaccgag gcgatccaca cgctgatgga
      721 ggggcgactg aatgacaccg aggtgatggt cagtagttat gtcgccaaga atggtgattg
      781 cgcctatgat ctggtctacg tggctccccc tgagctcttt caggaccggc tgccggaatt
      841 tgagcggttt gccaggggct gggtcttggc tgaaaaaggt tccaggttcc aagtaccggg
      901 ttccacgttg aggttaaggt aaagaactgc gacaaggaca gacaatgcgg aaggtagctg
      961 tagcagttgg aggccagtcg attcgattgc tcgaactgct gggcgggctt tcgaagctta
     1021 cctatgacac cgtttactgc gctttcacgc ctccctacaa cccccgtggg ctcctccagc
     1081 aaatcgatca tatcggggtg aaatcgatct cgatcgccag cgtggccgcg gtcttcaccg
     1141 gccttgtctt agcgctgcaa accgcgtacg gcctgagccg attcggggcg aaggcgtatg
     1201 tggggattat cgtctcgctc tcaatggtcc gcgagttagg gccggttctc acggcccttc
     1261 tggtgggagg gcgtgtcggt tccgggatca ccgccgaact ggggtcgatg aaggtcacgg
     1321 agcagatcga cgcaatgcgg gccatggggg cgaacccgat caaaaaactg gtggtacccc
     1381 gagtgctgag cgcgatgctg gtcctgccgc tgctgacggt catggcggac gtcctgggca
     1441 tgctgggggg tatggtcatc tcccggtacg agttccaggt cgattaccac ctctactaca
     1501 ataccgtgac acgcaacctg acggtggccg acattgtgag cggtctcggc aagacagtgg
     1561 tcttcggctt catcatcgcc atcgtcggct gctacagagg gctggaaaca gtgggcggca
     1621 cagaaggcat aggcagggcc acaaccgctg ctgttgtcac ctcggccatc gctgtcatta
     1681 tctcggattt ctttcttacg aagttcttct ggtggctgga gggctggtga gatgagcgcc
     1741 ccggccattg agttccgcca agtctacaag tcgttcaacc acacaccggt cttggccgga
     1801 atggatttca cgatccagtc gggtgagacg gtgaccatca tcggcggcag cgggatcggc
     1861 aagagcgtca ccttgaagct gatcgttggc ctgctcaagc cggagggggg ccggatactc
     1921 atcgaggggg aggacatcgt accgctggcg gaggaccagc tcatccgggt ccggaagaag
     1981 atcgggatgg tctttcaggg ttcggcgctc ttcgat
//
LOCUS       DATMXI010000365         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_292990, whole genome shotgun sequence.
ACCESSION   DATMXI010000365 DATMXI010000000
VERSION     DATMXI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292990"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..2181
                     /locus_tag="VN444_07040"
     CDS             535..2181
                     /locus_tag="VN444_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR21598.1"
                     /translation="MLRGWSKIDGIMAWVAITGGILIVLSIIVIVFLESRIVDQAGAE
                     DRQGGFLRAAEFVGKMISKTGGIRDLTVTEELIKEVRELRPGIKRLSVFEITSESASL
                     ILSSDPRAAPRTLGLQELAEIHAGRSVMQLDDSSEERAWRITAPIIVDGKVVGALRGL
                     YSIKEFDALKQEIGLTKVVGLGVGVVTSLTFLLLVRFRIHRPVYQILSAMRKVEEGNL
                     SVRAPVTGPAEIQEVASQFNRMLGCVREEGIEKDRLLEEVRHFNETLQKRVADATEEL
                     QQANLELVEARIAMERSQRLAALGELSATVAHELGNPLNALSGHLQMLSHTDDASSRQ
                     RHLAIIQSEVDRMVAIIRQVLNQTRVRLQSTPLNLNSTIHEVLSLLSPGMTRRHVTVK
                     TDLQDDLPLVAGDPRALHGLVFNLVSNAKQAMPSGGELMIRTRVAGGTELPGTVIVSE
                     GVLVNVGAVRLAIGDTGNGIPPEHLARIFEPFFTTRHEQGGTGLGLTMCHRVVTDSGG
                     RLAVKSDVGQGTEFTVDLPIWNERRVEDVTEWPAFNPRRR"
     gene            2141..>2480
                     /locus_tag="VN444_07045"
     CDS             2141..>2480
                     /locus_tag="VN444_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21599.1"
                     /translation="MSLNGLPSILVVDDDERNREMLAEALGQAGFEVETASAGMEALN
                     KAGGVAYDAVLSDIRMVPFTGLDLLDSLHKTMPEIPIVLLTAFGSVDTAIQAMKQGAF
                     DYIPKPVNLDE"
BASE COUNT          571 a          613 c          718 g          578 t
ORIGIN      
        1 ccattggctc tttttgcgtt gtatcagtct tcacttcgtg tttcttttct cttctccttt
       61 gagaagcctt acccgctcgt gaataattca ggcagcggtc gatagatgca ttgctgcttt
      121 ttcgtgcgac ataaagacgt acagttatcg atatatataa aaatattctg ccagcataga
      181 agtcttcatt acatcatagg tttatcgtaa caatacgcct actagaggcg agaggagcga
      241 gcctgctgct ctcttgtggc ccgacggaag ggcaacgcgt cgtgctacag gtagatgccg
      301 gcgtcaaacc ttgtctggat catgatttgc aatgcagcgg ctatgtaagg ttttgagaac
      361 gcttagagcc agaggctcgt ggctgaggca ataaaagtgt tttctcgtta gaatccgaga
      421 agatacgcgt tacgggtgtc tttctaattg ccaagagtca tggcgatgct gatgggacgc
      481 attaaatagt attatgatga gacgatgccg gggggtcacg tgaacgaggg agaaatgttg
      541 agagggtggt ccaagataga cggtatcatg gcctgggttg cgatcacggg aggcatcctc
      601 atcgtcttgt cgatcatcgt cattgtattt ctagaaagca ggattgttga ccaggctgga
      661 gccgaggatc ggcaaggagg gtttctacga gccgccgagt ttgtcggcaa aatgatcagc
      721 aaaactggcg gaatccgaga tctgacggtt acggaggaac ttatcaagga ggttcgcgaa
      781 cttcgaccgg ggatcaaacg tctctcggtg tttgagatca cgtccgaatc cgcttcgctc
      841 atactgagtt ctgatccacg agcagctccg cgaacactgg gcctgcagga actggcagag
      901 attcacgctg gtcgctcagt gatgcagctc gatgattcat cagaggaacg agcttggcgc
      961 attacagccc ctattatagt cgacggcaag gtcgttggtg ccctgcgcgg tctatattcg
     1021 ataaaggaat tcgacgccct taagcaggaa attggactga caaaagtggt cggcctcgga
     1081 gtcggcgtgg ttacatcgtt aacattcttg ttgctggttc gattcaggat ccatcggccc
     1141 gtgtaccaaa tactatccgc catgcgcaag gttgaagaag gaaatctatc tgttcgtgca
     1201 ccggtcacag gccctgccga gatccaagag gtggcatccc aattcaatcg aatgcttggt
     1261 tgtgtgaggg aagaagggat agaaaaggat cgccttctcg aagaggtccg gcatttcaac
     1321 gagacgcttc agaagcgcgt tgctgacgcg accgaggaac tgcagcaggc aaaccttgaa
     1381 ctggttgagg cgaggatagc gatggagcgc agccaacgct tggcagctct gggtgagttg
     1441 tccgcaacag tggctcatga attaggcaac cctttaaacg ccctctccgg tcatctacaa
     1501 atgctcagtc acacggacga tgcctcgagt cggcagcgac atcttgccat catccaatcc
     1561 gaggtcgacc gcatggtcgc catcatcaga caggtgttga atcagacccg tgtgcgcctc
     1621 cagtcgactc ccttgaatct caatagtacg attcacgagg tgctgtcgct gctctcacca
     1681 ggaatgactc ggcgacacgt gaccgttaag accgacttgc aagacgatct cccgctggtc
     1741 gccggagacc ctcgtgcctt acacggactg gtgttcaacc ttgtctccaa cgccaaacaa
     1801 gcgatgccat ccggggggga attgatgatc cggactcgcg tcgccggcgg tacagagctg
     1861 cccggaaccg tcattgtgag tgaaggtgtt cttgtgaatg tgggggcggt tcgcttggcc
     1921 atcggcgata cggggaatgg aatccctccg gaacacttgg ctcgaatttt cgaaccgttt
     1981 ttcacgacac gacatgagca aggtggtacc ggactcggct tgacgatgtg tcaccgggtg
     2041 gtgaccgaca gcggcggccg gcttgccgtg aagagtgatg tggggcaggg aacggaattc
     2101 accgtcgatc tgcctatttg gaatgagcgg agggtcgaag atgtcactga atggcctgcc
     2161 ttcaatcctc gtcgtagatg acgacgagcg gaatcgggag atgctcgcag aagccctagg
     2221 ccaagccggt ttcgaagtgg agaccgcctc tgctggaatg gaggctctga acaaggccgg
     2281 tggggtagcc tatgatgcgg tattgagtga catccgcatg gtcccgttta cggggctaga
     2341 ccttttggac tcgcttcaca agaccatgcc ggagattccc atcgtcctct tgaccgcgtt
     2401 cggttccgtc gacaccgcga tccaggccat gaaacaaggg gcgttcgact acattcccaa
     2461 accggtgaat ctcgatgaac
//
LOCUS       DATMXI010000366         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_182286, whole genome shotgun sequence.
ACCESSION   DATMXI010000366 DATMXI010000000
VERSION     DATMXI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182286"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VN444_07050"
     CDS             complement(<1..327)
                     /locus_tag="VN444_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21600.1"
                     /translation="MRFQRKLLLGFSLMVVPVLLISAQAIWNNREEREALRALGESMA
                     RTRTYADLETILFRQSRQVLGFLTGMDHEAKAEFFRLETEIGERLRQWKLTLTPDEMD
                     LAKDVEQ"
     gene            586..723
                     /locus_tag="VN444_07055"
     CDS             586..723
                     /locus_tag="VN444_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21601.1"
                     /translation="MRIQGQGKVKDVGLKALATMPALEGLDAKIALIQALIPLGFRPA
                     Q"
     gene            793..1626
                     /locus_tag="VN444_07060"
     CDS             793..1626
                     /locus_tag="VN444_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWR21602.1"
                     /translation="MICALTIASMPKVRIGQNEFFYQEDGHCEPVVWIHGLGIDHRVW
                     ALQMPLFTQHFRCLAFDNRDAGQSDRSPGSYTIKTLADDAIRLMDALAIDTAHIVGIS
                     MGGAVAQELAIAHPTRVRRLALVSTYTSSDRRGVDILNSFALMRARFSREEYARATSP
                     WVFTYQDYLIPGLAESVITRFLEDPYFLPADVYARQVEAARSHFTEDRLSQITAPTLI
                     VAGDEDLMTPMRFARTLHERIPGAKLEVIRGGGHALVLTHAEEFNRVVLSFLKGNYSG
                     S"
     gene            1815..2777
                     /locus_tag="VN444_07065"
     CDS             1815..2777
                     /locus_tag="VN444_07065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR21603.1"
                     /translation="MTGQNRRRYPAACCAVVHWLDSCLARSGIAFSLQSKHLSSYRGV
                     GEVMDASSRTTLDSYETYLVEHNRSMGCLVTIRRPFVLFTNDEIAVPWYNYAGVVPWS
                     EMPGRECIEEVMAHYRARGYSPHFTFTIETAPGGLTDALQSAGCALESHKYVMFQYEP
                     VDPPVPADVRLGKTGPEDMPAAGRILGVSFESGESAWQDPTVRIRLVARMRAARMHQL
                     GAWVDGQLAAAAHLHTGVGVGHITGVATAPQFRGRGLAGLLTAYAARFAREEGATLVA
                     LEVATPDAERVYARIGFRRAVERVAYSSKSQGTAPGLSPSGNPQ"
BASE COUNT          609 a          925 c          887 g          631 t
ORIGIN      
        1 ctgctcgacg tctttggcga gatccatttc gtctggcgtc agtgtcagct tccactggcg
       61 gagccgctcc ccgatctccg tctccaatcg gaagaactcc gccttcgcct catggtccat
      121 tccggtgagg aaccctaaga cttggcgact ctggcggaac aggatcgttt cgaggtcggc
      181 atacgttcgg gtcctcgcca tgctctcgcc gagggccctg agggcttctc gttcctcccg
      241 gttattccag atggcctggg cgctgatcag gagcacaggg acgaccatga gcgagaagcc
      301 gagcagcaac ttgcgctgga acctcatact tgaaactctc gtcttcctgt ttgagtggcg
      361 tcaagggaaa gtgatcgcgt gtaggggcag gcctcgtgcc tgcccagcgt ggtgaacgta
      421 cagcaatatt gcagtagggc tctgcgcatg actcctcgga tccgtccgaa ggtgtgccat
      481 tctttcattg agaattgacg aagcggctcc cgtgtggtga agtggttttg accaaataca
      541 ccctacaacc gcacaacgat aaggagccgt tccaggaaga gagtaatgcg aattcaggga
      601 caaggcaagg tcaaagacgt gggcctcaaa gcgctcgcca ccatgccagc gctcgagggg
      661 ctggacgcca agattgcgct gatccaggcg ctcatcccct tggggtttcg gccagcgcag
      721 tgaccttgac ggccaaggtc actgcgctga cagtctcgct tcgtccgtcc ctcgccagat
      781 tgattgacat ccatgatctg cgcccttaca atcgcctcta tgccaaaggt ccggatcggc
      841 caaaacgagt tcttttatca ggaagacggc cactgtgagc ctgtcgtctg gatccacggg
      901 ctcggcatcg accaccgggt ctgggcgctg cagatgccgc tgttcaccca acacttccgc
      961 tgcctggcct ttgacaaccg cgacgccggc caaagtgatc gctcaccggg ttcctatact
     1021 ataaagaccc tggccgatga tgccatccgc ctgatggacg cactcgccat tgatacggcc
     1081 cacatcgtgg gtatttccat gggcggggcg gtcgcccagg agttggcgat cgcccatcct
     1141 accagggtca gacgactggc ccttgtctca acctacacct cgtccgatcg acgcggcgtt
     1201 gacatactga actcgttcgc cttgatgcga gcccgcttca gccgggaaga gtatgcgcgg
     1261 gccaccagtc catgggtatt cacgtaccag gactatctta ttccgggatt ggcggagtcg
     1321 gtcatcaccc ggttcctgga ggatccatac tttctcccgg ccgacgtcta cgcccgccaa
     1381 gtcgaggccg cacgcagtca cttcaccgaa gatcgcttga gtcagattac ggcaccaact
     1441 ctgattgtgg ccggcgatga agatctcatg actccaatgc gttttgccag gactctccac
     1501 gagcgaatcc ccggcgctaa actggaagtg atccggggcg gcggacatgc cttggtcctc
     1561 acccacgccg aggagtttaa ccgcgtcgtc ctgtcattcc tcaaaggtaa ctactcaggt
     1621 agctaggctg aaggctgaag gtttttaggg acaaatcatg cgtgatcaca caaaacttcg
     1681 gaaagacctt gaatctactg ggggttcatt ccccgcggct tgccgcaagt tcgtcatacc
     1741 ggcggaagcc ggtatccaga gagcccgact ggattccccc atatcaagta cggggcaggc
     1801 ttgtcaagca cggaatgacg ggccagaaca gaagacgata ccccgcagct tgctgcgcgg
     1861 tagttcattg gcttgattcg tgccttgcga gatcaggcat agccttcagc cttcagtcta
     1921 aacacctgag cagctacagg ggggttggag aggttatgga tgcgtcgtct cgtactactc
     1981 tggattctta cgaaacctac cttgtcgagc acaaccgctc catggggtgc ctggtgacca
     2041 tcaggcgtcc gttcgtactt ttcacgaatg acgagatcgc cgtaccctgg tacaactatg
     2101 ctggcgtcgt gccgtggagc gagatgccag ggcgcgagtg catcgaggag gtcatggcgc
     2161 actaccgcgc gcggggctat agcccgcact tcacgttcac gatcgaaaca gcgccggggg
     2221 gacttaccga cgcattgcag tcggcaggct gtgcgctcga gtcccacaag tatgtcatgt
     2281 ttcagtacga gccggtcgac ccgccggtac cagcagacgt gcgtctgggt aagacaggac
     2341 ctgaggacat gccggccgcc gggcgcatcc tgggcgtcag cttcgaatca ggcgagtcgg
     2401 cgtggcagga tccaacggtg cgtatccggc tggtggcgcg catgcgtgcg gcgcgcatgc
     2461 accagctcgg cgcgtgggtg gatggacagc ttgccgcagc ggcacacctg cacacgggcg
     2521 tcggtgtggg gcatatcacc ggggtggcca ccgctccaca gttccgcggg cgcggtctgg
     2581 ccggcctgct caccgcgtac gccgcgcgct ttgcccgcga agaaggcgcc acgctcgtcg
     2641 cactcgaggt ggccacgcca gacgcggagc gtgtgtacgc gcgcatcggc ttcaggcgcg
     2701 cggtagaacg agttgcatac agttcaaaaa gccaagggac cgctcccggt ctttctccgt
     2761 ccggcaaccc gcagtgattg cgccaatcta ggaggtctat ctcgtctctt ccgtctattc
     2821 cgtcaccggc caaatagacg agctagacca aacagacgaa acaggccaga gagacctgcc
     2881 ggaccgcgtg aatttttttc ttgacactgc ccaggtcaac gcttaatctg atcccgttca
     2941 tcgatactga taatcgtttt cactgtcttt actatgcgag ctaaactcct ccaattccag
     3001 cagtaccttc atcgagccgg gctgaaggcg acccgccagc gcgagttgat cg
//
LOCUS       DATMXI010000367         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_212835, whole genome shotgun sequence.
ACCESSION   DATMXI010000367 DATMXI010000000
VERSION     DATMXI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212835"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VN444_07070"
     CDS             <1..1435
                     /locus_tag="VN444_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21604.1"
                     /translation="HYGGTGLGLTICSRLVELMDGRIWIESKPGQGSAFHFTAHFELQ
                     NQDEARKTRGALTLETLTGLSVLAVDDNATSRTILEEWLTAWQMQPTVAVSGREALEI
                     LRRAKETGRPINLCILDAEMPEVDGFSLAAEIMEQSQEKVPVILMLNSIDQPRDIARC
                     RDLGITAYMVKPITPSELWNAMMTALGRGGPSAKAAQKPEHGTNGDGSWISAAYGRRY
                     RVLLAEDNPINQMLAVRLLEKEGHSVLVAGNGLQALAALERGPFDLVLMDVQMPEMDG
                     LEATAAIREQERATERHLPIIALTAHAMKGDRERCLAAGMDEYLAKPLRAEALFGVIV
                     RLLAEPADASTSTSVVDPSAFSLQPSASTPESPFDEEELLERVGRDVGLMREIVGLFL
                     EEGPIRIIEIREAIDRGDAQALAKAAHTLKGAVSVFGARRSVEAALRLEQLGRAGDLV
                     QVGEGFRDLEVAMAGLLPALETLMGEE"
     gene            1501..1875
                     /locus_tag="VN444_07075"
     CDS             1501..1875
                     /locus_tag="VN444_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21605.1"
                     /translation="MRILIAEDSLVSRRLIETTLRRWGHEVIATSDGSEAWQALQAGE
                     RPVLAILDWLMPGMDGPELCRRIRETPDISSLYVILLTAKGTQEDVLDGLTTGVNDYL
                     VKPFDPEELRGRLEKARCIVES"
     gene            2158..>2814
                     /locus_tag="VN444_07080"
     CDS             2158..>2814
                     /locus_tag="VN444_07080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR21606.1"
                     /translation="MRRNKQKGAHSQVTERPTLSLCMVVRNEETNLSRCLESVKGVID
                     EIIIVDTGSTDRTVEIAQQYGAKIFSHRWDNDFAAARNVSLSHATSDWILVLDADETL
                     AEEDRGRLRALLRPDGPTAYLLNIVSPVDDRRSSHAVINAFPRLFRNRPEIRFEGRVH
                     EQVSPSIARMGGTVSPSEIRIHHQGYHSRWVDLSAKRQRNIHLLRRQLTDHPDDPSAH
                     F"
BASE COUNT          591 a          780 c          885 g          558 t
ORIGIN      
        1 gcattacggt ggaaccggat tggggttgac gatctgctcg cgcctggtcg aactgatgga
       61 tgggcggatc tggattgaga gcaaaccagg ccaagggagc gcgtttcact ttacggcgca
      121 ctttgagttg cagaatcagg acgaagcgcg taagacgcga ggcgccctga cactggagac
      181 attgaccggg ctatcggttc tggcggtcga tgacaatgct accagccgca cgatcctgga
      241 ggaatggctc acagcctggc agatgcaacc cacggtagcc gtttccggcc gtgaggcgtt
      301 ggagatcctg agacgggcaa aggagaccgg tcgtcccatc aacctgtgca tcctggatgc
      361 cgagatgccg gaggtagatg gtttctccct cgccgccgag atcatggagc agagccagga
      421 gaaggtcccc gtaattctca tgctgaactc gatcgatcag ccgagagata ttgcccggtg
      481 ccgggatctt gggatcaccg cctacatggt gaaaccgatc accccctccg agctatggaa
      541 tgccatgatg acggcgttag gcaggggtgg accgtcggcg aaggcggctc agaagccgga
      601 gcacggtacg aacggagacg gctcttggat ctctgcggcg tatggtcggc ggtatcgggt
      661 cctcctggcc gaggacaatc cgatcaacca gatgctggcg gttcgcctcc tggagaagga
      721 ggggcattcg gtattggtgg ccggtaatgg actccaggct cttgccgcac tggagcgggg
      781 accgttcgat ctggttctga tggatgtgca gatgccagag atggatggcc tcgaggcgac
      841 cgccgccatt cgggaacagg agcgtgcgac ggaacggcac ctcccgatca ttgccctgac
      901 ggcccatgcg atgaagggcg atcgtgagcg gtgcttggcg gccgggatgg atgaatatct
      961 cgccaaaccg cttcgggccg aggcgttatt tggtgtgatc gtgcgtctgt tggccgagcc
     1021 ggcagatgct tcgacatcaa cctccgttgt agacccctca gccttcagcc ttcagccttc
     1081 agcctccacc cctgaatctc cattcgatga agaggagctg cttgaaaggg tcggaaggga
     1141 cgttggcctc atgcgagaaa tcgttgggtt attccttgag gagggtccga ttcgcatcat
     1201 cgagatccgc gaggcgatcg atcgtggaga tgcccaggcc cttgcgaagg cggctcacac
     1261 actcaaaggg gcggttagcg tgttcggggc gaggaggagc gtagaggcgg ccctgagact
     1321 cgagcaactt gggcgcgcag gcgatttggt tcaggtcgga gaaggattca gagacctgga
     1381 ggtggcgatg gcaggcctgc tgccggcact cgagacgttg atgggtgaag agtaatggcg
     1441 ttaatcgtgt ctgttgagtc tgtttcactg gccactattc actgaaaggg gggagtaaac
     1501 gtgcggatcc tgattgctga agacagcctc gtctcgcgtc gcctgatcga aaccaccctc
     1561 aggcgttggg ggcacgaggt gatcgcgacc tctgacggct ccgaggcgtg gcaggcttta
     1621 caggctggtg agcggcccgt gcttgcgatc ctagattggt tgatgccggg gatggacggc
     1681 ccggagctct gccggaggat ccgtgagacg ccggatatct cctctctgta cgtcatccta
     1741 ctcacggcca aagggacaca ggaggatgtc ctggatggac tcacgacggg agtcaatgat
     1801 tacttggtga agccgtttga ccccgaggag ttgcggggca gacttgagaa agcacgttgc
     1861 atcgtcgagt cataacaaag tcggacctcc tgtaatgccc ccctcacctc gcacgtctcc
     1921 cctcgatgaa aatcaactac tcaggtgttg aggctgcagg ctacaggcta tctcgcaagt
     1981 cacgaatcaa gccattcaag ctcttctcgg ttcctacccc tgatagcctt tagggtttag
     2041 tctatctacc tgagtagttc caaaaaaacc gctcatgcct ccatcctcta gcgccgataa
     2101 aagtataaga agtgaaacgc ggtgcatggt cagagggatt ggacagtgga gcgaccaatg
     2161 agacgtaaca agcaaaaggg ggctcactca caggtcaccg agcgaccaac gctctccctc
     2221 tgcatggtag tcaggaacga ggagaccaac ctcagccgtt gcctggaaag cgtcaagggc
     2281 gttatcgacg aaattatcat cgttgatacc ggctcaaccg atcgaaccgt cgagatcgcg
     2341 cagcagtatg gggctaagat tttttcgcac cggtgggata acgacttcgc ggctgcgcga
     2401 aacgtctcat tatcgcacgc tacctccgac tggatcttgg tactggatgc ggacgagacc
     2461 ctggctgagg aggaccgggg ccgtctccgc gcccttttgc gtccggacgg tccgaccgcc
     2521 tatctgctga acatcgtgag cccggtggac gaccggcgca gcagccacgc cgtcatcaac
     2581 gccttcccgc gtctgttcag aaaccggccc gagatccggt ttgaagggcg ggttcacgag
     2641 caggtgtcgc cgtcaatcgc gagaatgggc gggacggtga gcccgtctga aatccggata
     2701 caccaccaag ggtatcacag ccgctgggtt gacctctccg ccaagcggca gcgcaacatt
     2761 cacctcctac gtcggcaact caccgatcat cccgacgacc cctcggccca cttc
//
LOCUS       DATMXI010000368         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_318342, whole genome shotgun sequence.
ACCESSION   DATMXI010000368 DATMXI010000000
VERSION     DATMXI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318342"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VN444_07085"
     CDS             <1..389
                     /locus_tag="VN444_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWR21607.1"
                     /translation="PSEAPVKPMAQQDVKIPAVKAPETVLKISGTAGSNPYWARVQSI
                     ISSHWEPPPVDMIGQTYTMVVKFRLQRDGVIKDVVVQQSSGNTYFDLAGQRAVLRPRI
                     LPAFPAEMADSYKDVEMAFRVGESVG"
     gene            386..1726
                     /gene="tolB"
                     /locus_tag="VN444_07090"
     CDS             386..1726
                     /gene="tolB"
                     /locus_tag="VN444_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWR21608.1"
                     /translation="MTRVKMISAWFGGVLFLAGAVGILESGAADVFLEVTRPDFQKIS
                     LGIAGIHNGGGPEWLGGRIEEVLKKDVKRSLVFDLVDLPSLGITPSNIGNGTGTGSKA
                     VFKQVAEKGVSVLVWGKAVMKEAQKDADVNMEGFVYDSGSDEVVGGKRYAGSPSVVRL
                     MAHRFVDELVFRYTGEPGIARTKIAYVAQQGTARELFVMDYDGYDARQLTADGFLNLM
                     PQWSPDRRFLVFTAYRDRNTQTIDMIELATGKRWTLISLGGLNITPALSPDGNFLAFA
                     SSHEGNSELYRLDTRTKTIQRLTVNPSGDLSPSWSPNGRELVFVSDRGGGPQLFLMGS
                     DGTNVRRLTFDGDYNAAPTWSPRGNWIAYVCRTQREYKLCVITPDGQKRMQLTTGPGV
                     DDSPSWSPDGRHLVFSSTVEGKSHIYMIDADGKDLERLTFQGTHNSAPSWSPAS"
     gene            1806..2465
                     /gene="pal"
                     /locus_tag="VN444_07095"
     CDS             1806..2465
                     /gene="pal"
                     /locus_tag="VN444_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWR21609.1"
                     /translation="MHKTLCTLGVALVASVALIGMPGCSKKSVQSGGDAQSTQGKADS
                     SKPGVGSYPDTSVQPGGGGLRGLDKNPSEERVGSGTMLAKVDPSSNAGRQIDEIRAEQ
                     AASAAAGLRDVFFAYDSWVITEEGRQALSRNAEWIKSNSNALLKVEGHCDERGTSAYN
                     LVLGEKRAKAVRNYLVELGVGANRLSVVSYGKERPSCTEHAESCYQQNRRGHLAVKVG
                     K"
BASE COUNT          584 a          601 c          790 g          576 t
ORIGIN      
        1 aaccgagcga ggcaccggtc aagccgatgg cgcagcaaga cgtaaagatt ccagcggtca
       61 aggctcccga gacggtcctg aagatttccg gtacggcagg gtcgaatccg tactgggctc
      121 gtgtccagtc cattattagt agccactggg agcctccccc ggtagatatg ataggccaaa
      181 cctatacgat ggtcgtcaag tttcggcttc aacgggatgg ggtgatcaaa gatgtggttg
      241 ttcagcagtc ctccgggaac acctattttg atctggcggg ccagcgtgct gtactgcgac
      301 cgcgaattct gcctgcattt cctgctgaaa tggcagacag ctataaagat gtagagatgg
      361 catttcgagt cggagaatca gtcggatgac cagggtgaaa atgatcagcg cctggttcgg
      421 aggggtactg ttcctggcag gagctgtcgg tattcttgaa tctggagcag cggatgtgtt
      481 cctggaggtc acccgccccg acttccaaaa gatttccctg ggtattgcgg ggatacataa
      541 cgggggaggt cccgagtggt taggggggcg aatcgaagaa gtcctcaaga aggatgtaaa
      601 acgctccttg gtcttcgatc tggtggattt gcccagtctt ggaattacac catccaatat
      661 cggcaatggg acagggaccg gttccaaggc tgtattcaaa caagtagctg aaaagggtgt
      721 ctcggtcttg gtctggggaa aagcagtcat gaaagaggcg cagaaggatg ccgacgtgaa
      781 tatggagggc tttgtctacg acagcgggag cgacgaagtt gtggggggga aacgctatgc
      841 ggggtctcca tcagttgtgc ggctgatggc gcaccgcttt gttgatgaac tggtatttcg
      901 gtacacgggt gagccgggca tcgcacggac caagatcgcg tatgtggctc agcagggaac
      961 tgctcgcgag ttgtttgtga tggattatga tgggtacgat gcgcgtcagc tcacggctga
     1021 tggatttctg aacctcatgc cgcaatggtc tccagatcgc cggttccttg tcttcactgc
     1081 ctatcgtgat cgtaatactc agaccatcga tatgattgag ttggcgacgg gtaaacgatg
     1141 gactcttatt tctctggggg gattgaatat cactccggcg ctgtcccctg acggaaactt
     1201 cctggcattc gcctccagtc atgaaggtaa ttccgaactc tatcggttgg ataccaggac
     1261 aaaaaccatc caacgattga ctgtaaaccc gtccggtgac ttgtcgcctt cctggtctcc
     1321 gaacggacga gagctggtat ttgtgtcgga tcggggcggc gggccgcagt tattcctcat
     1381 gggttcagat gggacgaatg tgcgccgcct gacgtttgac ggtgattaca atgccgcgcc
     1441 gacgtggtcg ccccgtggga actggattgc ctatgtgtgc cggacgcaac gagagtacaa
     1501 actctgtgtc atcacgccgg atggacagaa acgaatgcaa ttgacaacgg ggcctggtgt
     1561 cgatgattca ccatcctggt cgccggatgg tcggcatctc gtcttcagtt cgacggtcga
     1621 gggaaagagt cacatctaca tgattgatgc cgatggtaag gatctagagc ggctcacctt
     1681 ccagggcacg cacaatagcg caccttcctg gtcaccggca tcctgaggat ggaatagtga
     1741 ctctagcgga taaatgaagg taaaaggcag agcttattaa cgtcggaaat aaggagaagg
     1801 aagatatgca caaaacactc tgtacattag gtgtggcgct ggtcgcaagt gtggcactga
     1861 ttggtatgcc tggctgcagc aaaaaatctg tgcaatccgg cggcgatgcc cagtctactc
     1921 agggaaaggc ggatagttcc aagcccggtg tcggtagcta cccggacacc agcgtacagc
     1981 ccggcggtgg cgggcttcgg ggtctcgata agaacccttc agaggagcgt gtcggtagcg
     2041 gcacgatgct ggccaaggta gatccttcga gcaatgccgg acgacaaatc gatgagattc
     2101 gtgccgagca ggctgcgtcg gctgcagcgg gcttacggga tgtatttttc gcctacgata
     2161 gctgggtgat cactgaagag gggcgtcagg cattgagtcg caatgctgag tggatcaagt
     2221 ccaattcgaa tgccctgctc aaggttgaag gacactgtga cgagcgtggg acgtctgcct
     2281 acaacctggt actcggtgaa aaaagggcga aagccgtacg gaactacctg gtcgaactgg
     2341 gtgtcggcgc caatcgtctg tccgttgtct cgtatgggaa agagcggccg tcctgcaccg
     2401 aacatgcgga atcctgttac caacagaatc ggcgtggaca tcttgcggtg aaggtcggga
     2461 aataagcgac atgtagtttg tcgcctggct gtgactccgt tgtccttgaa caaagagtcg
     2521 tggattcctt tatcataagt atgggtggaa c
//
LOCUS       DATMXI010000369         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_18292, whole genome shotgun sequence.
ACCESSION   DATMXI010000369 DATMXI010000000
VERSION     DATMXI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18292"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(585..1457)
                     /gene="phnE"
                     /locus_tag="VN444_07100"
     CDS             complement(585..1457)
                     /gene="phnE"
                     /locus_tag="VN444_07100"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HWR21610.1"
                     /translation="MAHTPTQAAGLAGTKRRDLLSTPNLIFLAGFLVLMAWSYVGTEF
                     SLRGLLGGESAAQILTYAKKLFPPDLSTDFLLKTGYWILETFAMSFLGTILAVLIAFS
                     LVFLSSRNLMFTGLLFEMERSRPWVRALRTVLYLSAKAALNLLRTVPHLVWALILVFA
                     VGLGPFPGMLALGIHTGGVLGRLFGEVMENVETQPIEALQATGASRLQILFYGILPQV
                     LPEFVAYTLYRWEVNIREAVILGYVGAGGLGQQIQIAISLFLEHRLLTLILAIYLIVT
                     VVDSLSAYLRSRLA"
     gene            complement(1457..2344)
                     /gene="phnE"
                     /locus_tag="VN444_07105"
     CDS             complement(1457..2344)
                     /gene="phnE"
                     /locus_tag="VN444_07105"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HWR21611.1"
                     /translation="MQSNVPSTPASLTLPTRRFSTFQIVCSVLFILTFLGSYKLAQVK
                     PLLLFETEGRRNIWKFVTGMFPPDLSWNFLSLLGRPILETIQISLMGTFIAVVIGFPI
                     GLLATSSLTFKGILYERELAGSRPRAMLHKGSYLLARAILSLFRTIPEFVWAFMFVRA
                     VGLGPFPGVLAIAISYSGMLGKVYSEILEHVNPGPLEALQATGASRLTILLYGLLPQS
                     LPNLVSYTLYRWECAIRASAILGFVGAGGLGQQIEISMRMFNFNQVTTLVAILFVMVA
                     GVDALSARVRNAILGRSSV"
     gene            complement(2295..3113)
                     /locus_tag="VN444_07110"
     CDS             complement(2295..3113)
                     /locus_tag="VN444_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HWR21612.1"
                     /translation="MYLLDRVSKVFAGRIIAVDELDLKIRQGERVALIGPSGAGKTTL
                     FRMLNLTIPPTSGSLSFDGLDIGRLSGRRLREVRRRIGTIYQQHNLTPRLQVIHNVLA
                     GKLGTWPVWQAVRSLIRPTEVDLASLALRQVGIADKLYDRTETLSGGQQQRVAIARLL
                     VQNPEVILADEPVSSVDPALAAGIVKLLIDLSQTTRKTLVMNLHSVDLALAHFPRVIG
                     LKDGKISFDLAAPAVTDDHLAALYAGSQTEAAEEEFPIHAEQRSLYSCQPHLTN"
     gene            complement(3128..3964)
                     /locus_tag="VN444_07115"
     CDS             complement(3128..3964)
                     /locus_tag="VN444_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWR21613.1"
                     /translation="MAVVVSLGLLSPAWSDAPKELRVSAIPDENPTELMRIYTPFAEY
                     LSKELGIPVRYYPVVDYAATVEALAAKKLDMVWYGGFTFVQARKRTGNAIPLVSREED
                     LRFHSKFITRPETGIKTLADLKGKTFSFGSVSSTSGHLMPRYFLLRNGINPERDFATF
                     SFSGAHDATALWVESGKVDAGALNEAVWDKLVQTKKVDPNKVQVFWTTPPYIDYVWTV
                     RGDLDSSLVEKIAAAFTKLNYSNPADRALMDLQRTKRYVRVKVGQFKPAEEAAIAAGL
                     LQ"
BASE COUNT          946 a         1239 c         1336 g          888 t
ORIGIN      
        1 cgccgataca gcggttgtgg caagggtacg tgcaggttct tcatgtccgt cttgggcatg
       61 gcctatcctc ctgacagcaa gtatggcaga aaagatgcca tgcgtaaagt tcagttttcg
      121 gaaatcatgt agcagtagta aatgcactga gttagggttt gggcgtagtg gggtcggtca
      181 cataaccagc gcttcgaccg gacgcacgga acgcaccggt cacgcatccc gttcagcggc
      241 ttccgccttt gctcaatcgg aacttgccga ggagaattga tatgtctttc gcaagattcc
      301 gaatgccctt ataaagaccc ctcatgaggc gattcacctc tctgaaattt gcacaagaaa
      361 ccctgttctt ctcactctcg tctataccag atgcccgtac atttaggggg atttgaccgt
      421 ccggcgctgg ttgtgccgga actgatcggg ataggtatgt cgttttctgg ctgctttcgc
      481 gcgacgcagg acgtgcgggg tgaggtggcg ggcaatctcg gagaagacat gcccgggggt
      541 ggtcggttat cgtcatgtcg cctggtgtgt gtgtggtgaa aaagtcaggc aagtcggctt
      601 ctcaggtagg cgctgaggga atcgacgacg gtcacgatca ggtagatcgc gaggatcaga
      661 gtcagcaggc ggtgctccag gaagaggctg atcgcaatct ggatttgctg gccgagaccc
      721 cccgccccga cgtagcccag gatgaccgcc tcccggatgt tcacctccca gcggtacaac
      781 gtgtaggcga caaactccgg caggacctgg gggaggatgc cgtagaacag gatctgtaac
      841 cggctggcgc cggtcgcctg tagcgcctcg atcggttggg tctcgacgtt ctccatcacc
      901 tccccaaaca gcctgcccaa gactccgccc gtgtgaatcc ccagggcgag catccctggg
      961 aacgggccga ggccgaccgc aaagaccagg atcagcgccc agaccaggtg agggacggtc
     1021 cggaggagat tgagagcggc cttggcgctg aggtatagga cggtgcggag cgctcgaacc
     1081 cacggacggc tccgttccat ctcgaacagc agtccggtga acatcaggtt gcgactggag
     1141 aggaagacga gggaaaaggc gatcagtacc gccaggatcg tcccgagaaa cgacatggcg
     1201 aaggtctcga ggatccagta gccggtcttg agcagaaagt ccgtggagag atcaggcgga
     1261 aagagcttct tggcgtaggt caggatctgg gctgcactct ccccgcccag cagtccacgc
     1321 agcgagaact ccgttcccac atagctccag gccatgagga caaggaagcc cgccaggaat
     1381 atcaggttgg gggtggagag gagatcgcgc ctcttcgttc ccgcgagacc cgcggcctgc
     1441 gtaggagtat gtgccatcag acgctcgacc gaccaaggat ggcgtttctc actctggcac
     1501 tcagggcatc aactccagcc accatcacga acaggatcgc gaccagggtc gtgacctggt
     1561 taaaattaaa catccgcatc gagatctcaa tttgctggcc gagccccccc gcgcccacga
     1621 aacctaaaat cgccgatgcc cgaatggcgc actcccaacg atacagggtg taggacacca
     1681 ggttcggcaa ggactgtggc aacaggccat acaggaggat ggtgagccgc gaggccccag
     1741 tcgcctggag cgcttccaga ggcccaggat tcacatgctc caggatctcc gagtagacct
     1801 tccccagcat tcccgagtag gagatcgcaa ttgccaggac accggggaac ggtcccagcc
     1861 cgacggctcg gacaaacatg aaggcccaca cgaattcagg gatggtccgg aataggctga
     1921 ggatcgccct ggccagcaga tacgatccct tgtggagcat tgctcgcggg cgagagccgg
     1981 ccagctctcg ttcgtacaga atgcctttga aggtgagcga acttgtcgca aggaggccaa
     2041 tggggaagcc gatgactacg gcgatgaagg ttcccatcag ggagatctga atggtttcca
     2101 ggatcgggcg acccagcaga ctcagaaagt tccaggacag gtctggcggg aacatgccgg
     2161 tgacgaactt ccagatgttc cggcgtccct cagtctcgaa gagcagcagt ggcttcacct
     2221 gggcaagctt atagcttccc aggaacgtca ggatgaacag gacggaacag acgatctgga
     2281 aggtggagaa gcgcctagtt ggtaaggtga ggctggcagg agtagaggga acgttgctct
     2341 gcatggatgg ggaactcctc ttcggctgcc tcggtctgac ttccggcgta cagcgctgcc
     2401 agatggtcat ctgtcaccgc tggggctgcc agatcgaacg atatcttccc atccttcagc
     2461 ccgatcactc gggggaaatg ggccagcgcc agatcaacac tgtggaggtt catgacgagg
     2521 gttttgcggg tggtctggct cagatcgatc agcagcttga cgattccggc ggccagtgcg
     2581 ggatccactg aagagaccgg ctcgtcggcc aggatcacct ccggattctg taccagcagg
     2641 cgggcaatgg cgacccgctg ttgctggccg cctgacagcg tctcggtcct gtcgtacagc
     2701 ttatcagcga tgccgacctg tctgagcgcc agggaggcga gatcaacctc cgtcggtcgg
     2761 atcagggagc gcaccgcctg ccagacgggc caggtcccga gtttgcctgc aagcacattg
     2821 tggataacct gcaggcgagg ggtcagattg tgttgttggt agatcgttcc gatccggcgt
     2881 cggacctctc gcaaccgccg tccggagagg cggccgatgt ccagtccgtc gaatgatagg
     2941 cttcccgagg ttggggggat cgtgagattc aacatccgga acagggtcgt ctttccggcc
     3001 ccacttgggc cgatcaaggc cacccgctcg ccctgtcgga tcttcagatc aagctcgtct
     3061 acggcgatga tccggccggc gaagacctta gagactcgat cgagcaggta cataagttct
     3121 gatggcgcta ctgaagcagc ccagcggcga tcgctgcctc ttcagccggc ttgaactgcc
     3181 cgaccttgac gcggacgtat ctcttcgtac gctgaaggtc catgagggct ctatccgctg
     3241 gattgctata gttgagcttc gtgaaggctg cggcaatctt ttctacgaga ctgctgtcca
     3301 gatcgccccg cacggtccag acgtagtcga tgtagggggg tgtcgtccag aacacctgca
     3361 ccttattggg atcaaccttc ttggtctgaa cgagcttgtc ccacactgcc tcattcaagg
     3421 ccccggcgtc aactttcccg ctctcgaccc agagggcggt ggcatcgtgg gcgccgctga
     3481 agctgaatgt cgcgaagtct cgttccgggt tgatcccatt ccgcagaagg aaatagcgcg
     3541 gcatgagatg gcctgacgtc gagctgacac ttccgaagga gaaggtcttg cccttcaagt
     3601 cggccagggt cttgatgccg gtttccggcc tggtgatgaa tttactgtga aagcgcaggt
     3661 cctcctctcg gctcaccagc ggaatcgcgt tgcctgtccg cttccgagcc tggacaaagg
     3721 tgaacccgcc gtaccagacc atgtccagtt tcttggccgc cagggcttcg acggtggcgg
     3781 cgtagtccac tacgggatag tatctgactg ggatgcctag ttccttgctc agatactcgg
     3841 caaatggcgt gtagatccgc atcagctcgg tgggattctc gtccgggatg gccgaaaccc
     3901 gcagctcttt tggcgcatcg gaccaggcag gactgagcaa tccgaggctt accacaacag
     3961 ccaccagact tgataacccg acatgggtcc atcggtagcg cacggaatca ctcctaaagg
     4021 ctatatttac attctgttat gaaatggggt gaaaactgca ccgtacacta tcggctcaaa
     4081 tccgataagt caaattggaa aatcctatta ccttggctca gttcatcggc ccgctttctg
     4141 gcagtaacaa ggcggagatg gaggagttga agaaaaaagg ctcttcgcgt aatcgagtgg
     4201 gtgaatttaa catgaaatcc tctgtaagcc ttgtggcatc tggtaaaatc cggtctgcac
     4261 cccttgcaga aaacgggagg atttctcaat ggctatttca accaaacgca agcgcctgct
     4321 cgacgcatat cgctttcccg gtttccgtcc gctggaaact gtccggggtg tctttggcga
     4381 ccacaaggca cgggtcatca cgctcgtcc
//
LOCUS       DATMXI010000370         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_33529, whole genome shotgun sequence.
ACCESSION   DATMXI010000370 DATMXI010000000
VERSION     DATMXI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33529"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="hemG"
                     /locus_tag="VN444_07120"
     CDS             <1..775
                     /gene="hemG"
                     /locus_tag="VN444_07120"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HWR21614.1"
                     /translation="ADSGPRYSLFATLDNGLQALVDALVRRLPAGTVRLNCPVAGIAR
                     EEKCWTVRLKDGTDLETDGIILAVPAFQAALLTHDLDGDLARELEAIPYASSVTISLA
                     YRKEAIPHPLDGFGFVVPACEGRTIIACSFSSVKFTKRAPSGHALLRAFAGGALQPEP
                     FEWDDERLLVAVRHDLKDLLGIESAPLWSHMVRHPRSMPQYHVGHLARLATLERQLRR
                     WPTLKLAGNAYRGVGVPDAIRSGEAAADALLAELTSAQG"
     gene            907..>2034
                     /locus_tag="VN444_07125"
     CDS             907..>2034
                     /locus_tag="VN444_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21615.1"
                     /translation="MQARKVVTLCGFMAALIFSAGCATTMSKVAYIVSEGQGTTSVLV
                     QNADGSRPVRVPGLSGAISEIAMSPSGQRLAYVTGGTTTGLEQVFVADLDENNNIVGV
                     RRNFSDELSTARSFSPKFLDDNRLLYLSESQGDRILVLVDLLTGGKQKISAVSGSIDT
                     VIRYNIDGTEALFFGLNKEMRRYDLPTGPLRSVVANTGSTSGLAYEKMGIFENTLVFS
                     TQVTPEERAFSHMRLDGTELKAVAEPPAELVFPIVRLAPDRASRVQRFSLFDFTMVTR
                     PADEADLITLEGYEVTAQKVTERHLFWIVRDLRNPDLVRAVYAAKGLEDLRTGAHGRF
                     VYLASTKDLLVWYNPTNKKIWGVPLDQIGADPVDLSAGVARV"
BASE COUNT          384 a          599 c          646 g          405 t
ORIGIN      
        1 ggccgacagc ggcccgcggt atagtctgtt tgccacgctg gacaatggac tgcaagcgct
       61 tgtcgatgcc ctggtgaggc ggttgcctgc cggcacggta cggcttaact gcccggttgc
      121 cgggatcgcg cgggaggaga aatgctggac cgtccggctc aaggatggca ctgacctcga
      181 gaccgatggg atcattctcg ctgtccccgc cttccaggcc gccctactga ctcatgacct
      241 tgacggcgac ctcgcccgag agttggaggc catcccctac gcctcctcgg tcacgatcag
      301 cctcgcctat cggaaggagg cgatccccca tccgctcgat gggtttggat ttgttgtgcc
      361 ggcctgcgag gggcgcacca tcatcgcctg ctcctttagc agcgtgaagt tcactaaacg
      421 cgcaccatcg ggacacgccc tgctgcgcgc ctttgcgggc ggggcgctcc aacccgaacc
      481 gttcgaatgg gatgacgagc ggctactcgt cgcggttcgc cacgacctga aggatctctt
      541 ggggattgaa tctgctccgt tatggagtca catggtacgg caccctcggt cgatgccgca
      601 gtaccatgtg gggcacctgg ctcggctggc aacgctggaa cgtcagcttc gccggtggcc
      661 aaccctcaaa ctggccggca acgcgtaccg cggcgtcggc gtccccgacg cgattcgtag
      721 tggcgaagcg gctgccgatg ccctgctggc agagttgacc tcagcgcagg ggtgacgctg
      781 accgagccag cccatacttg tgccgaagaa gattctctcc tataatcctg tacccggagg
      841 ctgaaaacga agaggcggat cggtccagtg agtgattccg ccgcgaacgc gaaaaggaga
      901 tcgccgatgc aagcaagaaa ggtcgttacc ctgtgtgggt tcatggccgc tctaatcttc
      961 agtgcgggtt gtgcgactac gatgtcgaag gtcgcctaca ttgtctcaga aggccaggga
     1021 acaacctcgg tcctggttca gaacgcagat ggtagcaggc ccgtccgcgt cccgggcctg
     1081 tccggggcaa tctcggagat cgccatgtca cccagtggcc agcggctggc ctatgtgact
     1141 ggcgggacca ccaccggcct cgagcaggtg ttcgtcgccg atctggatga aaacaataac
     1201 atcgtagggg tcaggcgcaa cttctcggat gaactgtcga ccgcgagaag cttctcgccg
     1261 aagttcctcg acgacaatcg cctcctctat ctttcggaga gccagggtga ccggattctg
     1321 gtcctggtag atttgctcac cggaggcaag cagaagatct ccgcggtcag cggatcgatc
     1381 gataccgtga tccggtacaa cattgatggc acggaggccc tcttctttgg gctgaacaag
     1441 gagatgcgcc ggtatgatct cccgaccggg ccgcttcggt cggttgtggc gaatacgggg
     1501 agtacctccg ggctggccta cgagaagatg gggatttttg agaataccct cgtgtttagt
     1561 acccaggtca cccctgagga acgggccttc tcgcacatgc gtctggatgg aacagagctc
     1621 aaggctgtgg cggagccccc tgctgaactg gtctttccga tcgtcaggct ggcaccggat
     1681 cgggcgtcgc gagtccagcg gtttagttta tttgacttca ctatggtcac gaggcccgcg
     1741 gacgaggcgg acctgattac gctggaaggc tacgaggtaa ccgcgcagaa ggtcaccgaa
     1801 cggcacctct tctggatcgt gcgagatctg cggaatccgg acctggtccg tgcggtgtat
     1861 gcggcgaagg gcttagagga cctcagaaca ggcgcccatg ggagattcgt ctacctcgca
     1921 tcgacgaagg atctgctggt ctggtataat cctaccaaca agaagatatg gggtgtgccg
     1981 ctcgaccaga tcggggctga tccggtcgat ctctcggccg gcgtggcccg ggtg
//
LOCUS       DATMXI010000371         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_213354, whole genome shotgun sequence.
ACCESSION   DATMXI010000371 DATMXI010000000
VERSION     DATMXI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213354"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..663)
                     /locus_tag="VN444_07130"
     CDS             complement(289..663)
                     /locus_tag="VN444_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HWR21616.1"
                     /translation="MRLKLDENLDPRTVGLLHSAGHDVVTVPDERLSGQPDTAVEAAC
                     RQEARCLVTLDLDFANVLAYPPERYSGLVVLRHPKPTAAGVLNLVRQFAAWLERENPE
                     RRLWIVEPGRLRVHEPNFKAEE"
     gene            complement(660..902)
                     /locus_tag="VN444_07135"
     CDS             complement(660..902)
                     /locus_tag="VN444_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HWR21617.1"
                     /translation="MKDWRERISIDPNVCHGKPCIKGTRIMVWIVVDYLANGDSVEEV
                     LAAFPSLTRDDVQAALAYAAEMTRERVIPVEVVGTP"
     gene            complement(1214..2143)
                     /gene="argF"
                     /locus_tag="VN444_07140"
     CDS             complement(1214..2143)
                     /gene="argF"
                     /locus_tag="VN444_07140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWR21618.1"
                     /translation="MKKDLLSISDLTVSEIDSLFFLAADLKAQQRKGVVHPLLPGKAL
                     GMIFEKPSLRTRVTFEVGMAQLGGRAIYLAPSEIQLGVRETVKDVAKNLERWVDGIVA
                     RTFAHRTVEELAAHAVIPVINGLSDLTHPCQILADLFTLQERCGTLHGVKVAYIGDGN
                     NVCHSWLYGAARTGIELNVACPQGYEPDADVVAASRQEAEASGGRIALLNDARAAAEG
                     ADVLYTDVWTSMGQEAEAAKRRHDFQRFQVDAALVRLAKPDVLVMHCLPAHRGEEITD
                     EVLDGPHSIVYDEAENRLHVQKAILVTLLKGSS"
     gene            complement(2140..3339)
                     /locus_tag="VN444_07145"
     CDS             complement(2140..3339)
                     /locus_tag="VN444_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWR21619.1"
                     /translation="MDQTTELMAQAARWLANTYARFPVALVKGSGVRVWDAEGKTYLD
                     FAAGIAVDVLGHCHPKVVEAIRVQAETLLHVSNLYYIEPQIRLARALSEQSFGGKVFF
                     CNSGAEANEAAIKLARRYATARWSSDRHEIVCMRDSFHGRTMATVTATGQSKYSQGFE
                     PLLPGFKHIPFNDLAAAERAIDSRTCAVLVEPIQGEGGVRVPDDDYLPGLRQLCSERE
                     VLLMLDEVQTGMGRTGRLFAYEHSGIEPDVMTLAKGLGGGLPIGAMIAKETVADAFVP
                     GSHASTFGGNPFVTTVALTVLTEILDARLSDRAAKIGAYFLERLQQLVTRYSFVKEAR
                     GKGLMLALELTVPAKPIVDRCLQRGLLILIAGDQALRFVPPLIIGEAEVDEAIRILDL
                     VLAEKSS"
     gene            complement(3417..>3681)
                     /gene="argB"
                     /locus_tag="VN444_07150"
     CDS             complement(3417..>3681)
                     /gene="argB"
                     /locus_tag="VN444_07150"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWR21620.1"
                     /translation="AEKLIFLTDTDGILDKDDALIPTLNRQRVEQLVADGVISGGMLP
                     KARACLTALDNGVEKTHIVNGTIPHALLLEFFTSEGVGTEIVG"
BASE COUNT          734 a         1159 c         1055 g          733 t
ORIGIN      
        1 aaacgggggc gggttcgtcg ccggggtcct gtgggaccag ttcgccgcgg aaggcccggg
       61 tcagaatgga ctgatcgagc ttgtctgcct gccgccgagc cgcccccact gccgcctcga
      121 tcgcatcagt gcagatcaac aggtcttcga tctttggggc gacctgctac tcggagaaaa
      181 gggacacgct agccagcact gtgcatgttg attgagggaa atcggcaatc cgaagtattc
      241 tctgttgtcg tcttccggct ctccgccatg gctggccgtt cccaatggtc attcctcggc
      301 tttaaagttc ggctcgtgaa ctctgagccg gccaggctcg acgatccaca atctgcgctc
      361 ggggttttcg cgctccaacc aagcggcaaa ttggcgaacc agattcagga caccggctgc
      421 tgtcggtttg ggatgtcgta ggaccaccag tccggaatat ctctcgggcg gataggcgag
      481 cacgtttgca aagtcgagat cgagcgtcac gaggcaacgc gcttcctggc gacaagccgc
      541 ctccacggcg gtatccggct gacctgaaag gcgctcatcg ggcacagtga cgacatcgtg
      601 gcccgcactg tgcaggagtc ctacggtgcg gggatccagg ttttcgtcga gcttcagcct
      661 cacggggtcc ctacgacttc cacagggatg acgcgctcgc gggtcatctc agcggcgtag
      721 gcgagcgcgg cctgaacgtc atcccgagtc agagacggaa aagctgcgag cacctcctcg
      781 acggaatccc cattggcaag gtaatcgacg acgatccaga ccatgattct ggtgccctta
      841 atacagggct tcccgtggca cacgttagga tcaatcgaaa tgcgttctcg ccagtccttc
      901 accttccctc tcctctgcat cccctcccac tagaggggag atcaatcttt aatctctttg
      961 ccattctcct ggagcttgag cgccagatcc cggagggcgc caaaggcggt ctccgactgg
     1021 gtaagagcct cgctgatcaa atccttctcg gtactggcgc tggcgtccta ctggtatgga
     1081 tggagaagct ggtcgttaac cggaggttgt tgaagctagg tacgtacagc ggtaatatca
     1141 cgagagagca actcctgctc gaccgacatc tttagcttct tactcagttg ttgcggatca
     1201 ccacgtgcga gcctcaagag gaacccttga ggagcgtcac caggatagcc ttctgcacat
     1261 gcagtcggtt ctcagcctcg tcatagacga tcgaatgcgg accatcgagg acctcgtcgg
     1321 taatctcttc gccgcggtgg gccggcaggc agtgcatgac cagcacatcg ggtttggcca
     1381 gtcggacgag cgccgcatcg acctgaaacc gctggaagtc gtgccgccgc ttcgccgcct
     1441 ctgcctcctg gcccatgctg gtccagacat cggtatacag gacatcggct ccctccgccg
     1501 cagcccttgc atcgttcagt aacgcaatgc gtccaccgga ggcctcggcc tcctggcgag
     1561 aggcggctac aacgtcagcg tccggctcgt acccttgggg acaggccaca ttgagctcga
     1621 ttccggtcct cgccgcgccg tacagccatg aatgacagac gttattgccg tcaccgatgt
     1681 aggcgacctt tacgccgtgc agcgtgccgc accgttcctg aagggtaaag agatccgcga
     1741 gaatctgaca cgggtgggtc aggtcgctca gcccgttgat gaccgggatc accgcatgag
     1801 cggccagttc ctccacggtc cggtgggcga aggttcgcgc gacaatccca tcaacccatc
     1861 gctcgagatt cttcgctaca tccttgacgg tttcccgtac cccaagctgg atctcggagg
     1921 gcgccaaata gatggcgcgg ccgcccaact gggccattcc gacctcaaag gtgactcgag
     1981 tcctgagcga cggcttctcg aagatcatgc ccagtgcctt gccgggcaac agtgggtgga
     2041 ccaccccttt tcgctgctgc gccttcagat cagcggccag gaaaaatagt gagtcgatct
     2101 cggagactgt cagatcactg atcgacagaa gatccttttt catgacgatt tctccgccag
     2161 aaccagatcg agaatgcgta tcgcctcatc gacctcagcc tcgccgatga tcagaggcgg
     2221 cacgaaacgc agggcctggt cgcccgcgat caggatcagc agaccgcgct gtagacatcg
     2281 atcaacgatc ggtttagccg gtaccgtcag ctccagggca agcatcagcc ccttgccgcg
     2341 ggcctctttc acgaaggagt atcgcgtaac cagttgctgc agacgttcga ggaagtaggc
     2401 accgatcttt gccgctcggt ccgataacct cgcgtcgagg atctcggtca gaaccgtcag
     2461 agccacggtt gtgacgaacg ggttgccgcc gaaggtcgac gcatggctgc caggtacaaa
     2521 ggcgtcagca acggtctcct ttgcgatcat ggcgccgatc ggcagtcctc cgcccagccc
     2581 cttggcgagc gtcatgacat cgggttcgat accggaatgc tcataggcga agaggcgtcc
     2641 ggtccgcccc atccctgtct gaacctcatc cagcatcagg aggacctccc gctccgagca
     2701 gagttgtcga agacccggca ggtagtcatc atccgggacc ctcacgcccc cctcgccctg
     2761 gatcggctct accagcacgg cgcaggtccg agagtcgatc gcccgctccg ccgccgccag
     2821 atcgttgaac gggatatgct tgaaacccgg caagagcggc tcgaaaccct ggctgtactt
     2881 cgactgaccg gtggcagtca ctgtggccat cgtccggccg tggaacgagt cccgcatgca
     2941 gacgatctcg tgtcggtcgg agctccaccg cgccgtggcg tagcggcgag ccagcttgat
     3001 ggccgcctca ttcgcctctg ccccgctgtt gcagaagaag accttgccgc caaacgactg
     3061 ttcgctcagc gcgcgggcca atcgaatctg cggctcaata tagtagaggt tcgagacgtg
     3121 cagcagcgtc tcggcctgca ccctgatagc ctctaccacc ttggggtgac agtgacccag
     3181 gacatccacg gcgataccag cggcgaagtc gaggtacgtc ttaccctcag cgtcccagac
     3241 ccgcacaccg ctgcccttca ccagcgccac cggaaagcgg gcataggtgt tggccagcca
     3301 gcgagccgcc tgcgccatca attcagtcgt ttggtccatc tggtctctct ggtccatcta
     3361 gtctatctgg tctgaccaaa tagacgaaac agacggaata gactaaatag acttacttat
     3421 ccaacgatct ctgtgccgac cccctctgac gtgaaaaact ccagcagaag ggcatggggg
     3481 atggtgccgt taacgatgtg cgtcttctca accccgttat ccagcgcggt gagacaggcc
     3541 cgggccttcg gcagcatccc tcccgagatg acgccatcgg cgacaagctg ttctaccctt
     3601 tgcctgttca gcgtaggaat aagggcgtca tccttgtcca gaataccgtc ggtatcggtg
     3661 aggaaaataa gcttttccgc t
//
LOCUS       DATMXI010000372         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_262485, whole genome shotgun sequence.
ACCESSION   DATMXI010000372 DATMXI010000000
VERSION     DATMXI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262485"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN444_07155"
     CDS             <1..412
                     /locus_tag="VN444_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWR21621.1"
                     /translation="RVQLKRLCPKATFVAAAVLSDHELTFSGNSRVWGGGTANIREKP
                     GKQVEGVAWEISEAERKALDEYEGYPGLYVRREVRVQTRSGKIIAAFAYIMGNPDRET
                     PPSKRYKQLLISGAEEHGLSDEYVDFLESIRPLT"
     gene            431..1903
                     /gene="gatB"
                     /locus_tag="VN444_07160"
     CDS             431..1903
                     /gene="gatB"
                     /locus_tag="VN444_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWR21622.1"
                     /translation="MSTTSTYEAVIGLEVHAQLLTRSKIFCGCSTVFGAPPNSQTCPV
                     CLGMPGALPVLNRRVVEFAIKSALALGCDIAPVSRFHRKNYFYPDMPKNYQISQYELP
                     LAQRGTIAFPVGGVITHVRIHRLHLEEDVGKLLHAGTLQVADSSLVDFNRSGVPLMEI
                     VSEPDIRSPEEAAEYLRQLRAILVYLEVCDGNMDEGSLRCDANVSLRLTGREELGVKA
                     EVKNMNSFKNVQKALAYEIQRQTQILEEGGRIVQETRLWDADQELTLPMRSKEHAHDY
                     RYLLEPDLVPLTVSPQWIDEIRATLPELPQQRRARFAREYGIPDYDAAVLTTSRPLAD
                     YYEEVATASHEPKLASNWVMVELLGHLNKDSRGITNSPIPPAELAALLTLLLRGTISG
                     KIAKMVFEQMYQTGKSAELIVKEQGLTQISNQDELLQIVEEVLAKHPGPVADYRKGKV
                     QSLTFLVGMVMKSSRGKANPQVAGELLLTRLKGEEPGGDS"
     gene            1905..>3114
                     /locus_tag="VN444_07165"
     CDS             1905..>3114
                     /locus_tag="VN444_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWR21623.1"
                     /translation="MTRSGRWWLLVLGGLVVVLFVGPLLLNQERYRGIFTSRASQLLN
                     RTVTTSSLRVHLLPSPGVTIRDLHIADRAPWSDAFLDAEQVTIGLKLLPLLKGELQVG
                     NVRIDRPRIRLAKGPDGWNLDDLIRPAPRAATAEPHRPEGTRVARGHPALPVLAAGGL
                     TIRHADLIFENPLHPSGPANLELKDVSLEIPTPLPPAPLRIHASGTLPGKLSGSFDLT
                     GSIQRSEGNHPPIEVELYVRNLETTQLASLFGLSQSSSLAAFSGTIGLTGKAVGEWPL
                     LDLQAEADLRQVGVALPRLQHAGTGAKDTGKASGENAWLRTKGRWDGDTLDLSEMNLL
                     WKGHTTTGRLHLETRRSPRLQFWLNTPDLSIEPFVALATTTGAATDRSNSAGTPYPAL
                     HVSNPPVPPFD"
BASE COUNT          666 a          947 c          929 g          572 t
ORIGIN      
        1 gcgggtccag ctcaagcggc tctgtccgaa ggcgacgttt gtggccgcgg cggtactctc
       61 cgaccatgag ctcaccttct ccgggaactc ccgggtgtgg ggcggcggga ctgcaaacat
      121 tcgcgaaaag ccagggaagc aggtcgaggg cgtggcgtgg gagatcagtg aagcggaacg
      181 aaaagcgttg gatgagtacg aagggtaccc cggcctgtat gttcgaaggg aggtccgggt
      241 acagacccgc tcaggcaaga tcatcgcagc gtttgcgtac atcatgggga atccggaccg
      301 cgagacgccc ccttcaaaac gctataagca gttgctgatc agcggcgccg aggaacacgg
      361 gctgtccgat gagtacgttg actttctcga gtcaatccgg cctctgacgt agagggcgaa
      421 ggagcggtaa ttgtccacaa catccaccta tgaggcggtc atcgggctgg aggtgcacgc
      481 ccagcttctg accagatcga agattttctg cggctgcagc accgtctttg gcgccccgcc
      541 aaatagccag acctgtcccg tctgcctggg gatgcctggc gccttaccgg tcctgaacag
      601 acgggtggtg gagttcgcca tcaagagcgc gcttgccctg gggtgcgaca tcgctccagt
      661 gtcccgcttc caccggaaaa attactttta cccggacatg ccgaagaact atcagatctc
      721 tcagtacgaa cttccgctgg cccaacgtgg aacgatcgca ttcccggttg gcggcgtcat
      781 cacgcacgtc cgaatccatc gcctccatct cgaagaggac gtcggcaagc tgctgcatgc
      841 tggtacgttg caggtcgccg actccagcct ggtggacttt aaccgcagcg gcgtgccgtt
      901 gatggagatc gtcagcgagc ccgacatccg cagcccggag gaggccgcag aatatctcag
      961 gcagcttcga gcgatccttg tgtacctgga agtctgcgat ggcaacatgg atgaaggcag
     1021 cctgcgctgc gatgccaacg tctcgttgcg actgaccggg cgagaagaac tgggggtcaa
     1081 ggctgaagtc aagaacatga actccttcaa aaacgtgcaa aaggccctgg cctatgaaat
     1141 ccagcgacag acccagatcc tcgaagaagg gggacggatc gtccaggaga cacggctgtg
     1201 ggacgccgac caggaactga ccctgccaat gcgaagcaag gagcacgctc atgactaccg
     1261 ctacctcctc gagccggacc tggtaccgct gaccgtctca ccgcagtgga tcgacgagat
     1321 ccgcgcaacc ctgcccgaac tgccgcagca gcgacgcgcc cggtttgcca gggagtacgg
     1381 gatcccggac tacgatgccg cggtcctgac cacctccagg ccgcttgctg actactacga
     1441 ggaggtcgca acagcctccc acgagccaaa gctcgccagc aactgggtga tggtagagct
     1501 cttgggccat ttgaataaag atagccgggg gattaccaat agcccgatcc cgccggcaga
     1561 gctggccgcc ctcctcacac tgcttcttcg tggaacgatt agcggcaaga ttgccaagat
     1621 ggttttcgag caaatgtatc aaaccggaaa atctgctgag ctgatcgtca aggagcaggg
     1681 actgactcag atctcgaacc aggacgagct actccagatt gttgaggagg tgctcgccaa
     1741 acatcctggg ccggtggctg actaccgcaa aggcaaggtg caaagcctta ccttcctggt
     1801 tggtatggtc atgaagtcca gtcgcggcaa ggccaaccca caggtggcgg gcgagctgct
     1861 tcttacgcga ctcaagggag aggagcctgg aggggacagc taaggtgaca agaagcgggc
     1921 ggtggtggct cctggtcttg ggtgggctcg tcgtggtgct cttcgtcggt ccgcttctgc
     1981 tgaaccagga gcggtaccga ggaatcttca ccagccgagc cagccaactg ctgaaccgca
     2041 cggtgaccac gagcagcctg cgagtgcacc tcctgccgtc gccaggggtg accatccggg
     2101 acttacacat tgccgatcgc gccccgtggt ctgacgcgtt cctggatgcg gagcaagtga
     2161 cgatcggact caagctgcta ccgctgctca agggcgaact ccaggtcggg aacgtccgta
     2221 tcgaccggcc gcgcatcagg ttggccaagg ggccggatgg gtggaacctc gacgatctga
     2281 ttcgaccggc cccgcgagca gcaacagccg aaccgcatcg ccctgaaggc acgagagtcg
     2341 cgagggggca tcctgccctg ccggtcctgg cggcaggagg tctgaccata cgccatgcgg
     2401 acctcatttt tgagaaccct ctccaccctt ccggaccggc caaccttgaa ttaaaggatg
     2461 tcagcctgga gatcccgact ccacttcccc ctgcgcctct ccgtatccat gccagtggaa
     2521 ccctacccgg caagctgtcc ggctcatttg atctgaccgg aagcatacaa cgttctgaag
     2581 gaaaccatcc gccgattgag gtagagttgt acgtgcgaaa ccttgagact acgcaactgg
     2641 catcgctctt cggcttatca caatcctcct ctctcgcagc attcagtgga accatcggtc
     2701 tcactggaaa agccgttggc gagtggcctc tccttgatct gcaggctgaa gcggatctgc
     2761 ggcaagtcgg cgtggcgctt ccccgcttac aacatgcggg gacaggcgca aaggacacag
     2821 gtaaggcgtc cggggagaac gcatggctgc gaacgaaggg acgatgggat ggcgacacac
     2881 tcgacctttc tgagatgaac ctgctttgga agggtcatac caccacgggc cggcttcacc
     2941 tcgaaacccg gaggtcgcct cgtctccagt tttggctcaa tacccctgac ctctccatcg
     3001 aaccttttgt cgctctggca accacgacag gcgcggcaac ggatcgttcg aattctgcag
     3061 gcaccccata ccccgcactc cacgtttcaa atcccccagt gccccccttt gata
//
LOCUS       DATMXI010000373         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_125292, whole genome shotgun sequence.
ACCESSION   DATMXI010000373 DATMXI010000000
VERSION     DATMXI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125292"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN444_07170"
     CDS             <1..521
                     /locus_tag="VN444_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HWR21624.1"
                     /translation="TIQVCLAYVDAQREYARMTREGDGLLTYAMKFRSDEGKHNGLYW
                     PIKEGEGLSPFGVLVANARAEGYSRKKSGSKPTPYHGYVYRILTAQGPDAPGGAYDYV
                     VNGKMIGGFALIAYPAQYGASGVMSFLVNHQGVIYEKDLGPDTEHLTRAMQTYNPDQT
                     WKQAGTSPPPAR"
     gene            complement(822..1250)
                     /locus_tag="VN444_07175"
     CDS             complement(822..1250)
                     /locus_tag="VN444_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HWR21625.1"
                     /translation="MLPVTVLPTINAVLNGTSALLLGLGYLLIRQRKITAHKLCMGSA
                     LGTSTLFLISYLAYHYQVGSMPFTGHGGIRALYFTILISHTVLAAVIPPLALITVSRA
                     LRGRFDRHVRIARWTLPLWLYVSVTGVIIYLMLYHLYPPA"
     gene            complement(1415..2320)
                     /gene="cyoE"
                     /locus_tag="VN444_07180"
     CDS             complement(1415..2320)
                     /gene="cyoE"
                     /locus_tag="VN444_07180"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWR21626.1"
                     /translation="MMLTSETLSAEYTHAGRRAADFVLLMKPRIVMMVLLTTFVGFYL
                     GVSGTPDYAALLQVLTGTALAAGGTLALNQFLERDIDARMVRTRLRPLPAGRLRPTEA
                     LLFGLLIVVTGLLYLAILVNALSGVVAAAIVGSYLFLYTPLKRRTAFCTVVGAVPGAL
                     PPVIGWAAATGRLGIEAWVLFAIMFLWQLPHTLAIAMLYKDDYRRAGIRLLPMIDPDD
                     KLTGCQIVCDCMVLLVVSLLPTLIGFAGPVYFVGALTLGIGLLVCACAFILWRSTTDV
                     RRLALASLVYLPALLLLMVLDRAPF"
     gene            complement(2317..>2561)
                     /locus_tag="VN444_07185"
     CDS             complement(2317..>2561)
                     /locus_tag="VN444_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21627.1"
                     /translation="LLIGLLVLQLLLGLGSYINRFTSVEIPYAAFTRLALPATHRITG
                     ALMLAVCIVLTLRAYRLPASRQAAPSQGLLAERVPV"
BASE COUNT          613 a          755 c          738 g          455 t
ORIGIN      
        1 ataccataca agtctgcctg gcctacgtcg atgcccagcg tgagtacgcg cggatgaccc
       61 gcgagggtga cgggctgctt acatacgcga tgaagttccg tagcgacgaa ggcaagcaca
      121 acgggctcta ctggccgatc aaggaaggcg aaggactgag cccatttggc gtactcgtcg
      181 cgaatgccag ggcggaaggc tactcccgca agaaatcggg gagcaagccg actccctacc
      241 atggctacgt ctaccggatc ctgacggctc agggcccgga cgcgccgggt ggcgcctacg
      301 actacgtcgt gaacggcaag atgatcggtg ggttcgcgct tatcgcgtac cctgcgcagt
      361 acggggcctc gggcgtcatg agcttcctcg tgaatcacca gggcgtcatc tatgagaagg
      421 acctcggccc ggacacggaa cacctcactc gcgccatgca gacgtacaac ccggatcaga
      481 cctggaagca agcaggaacg tcaccgccac ctgcccgtta gctgacatcc gccaaggcct
      541 cacgaaagat cctcagactc gaccccgcgg agtgggcaga cgagcgcacc accctacaat
      601 ttcgcggcta tttcacaagt gctgctcaca attcatttcg gcatatccat ccggatagcc
      661 gagatttacc tggtggggcg cagcaagcgt tcagcaatcg gttttcagct tctgaactga
      721 agtccctccg ttgaagcctc caactctcgg ctgtcaacct aagaccaaaa gctgattcgt
      781 gtcttcctgt tcgttgagtg ctgatcgctg aatgctaatc gctaggctgg cgggtagagg
      841 tgatacagca tgaggtagat aatgacgccg gtaaccgaga catatagcca cagcggaaga
      901 gtccaccgcg caatcctcac gtgtcggtca aagcgccctc tcagcgcgcg agagaccgtg
      961 atgagggcta atgggggaat caccgcggcc aggacggtat gggagatcag gatcgtaaaa
     1021 tacagcgctc gaatgcctcc gtgccctgta aaaggcatcg atcccacctg atagtgatac
     1081 gccagatagg agatcagaaa gagagtggag gttccaaggg ccgaccccat acagagtttg
     1141 tgcgcagtaa tctttcgctg tcggatcaac aggtagccga ggcccagcaa aagcgcgctg
     1201 gtcccattga gtacggcatt gatcgtaggc aggacggtaa ctgggaggat gactacgtct
     1261 cctgaaggag ggcttttcta gacagattca agactatcgt taggatggct catggcacga
     1321 aagcatggct acgcttcggt cctccacccc ccatcctcgc cttcccactc atgggggggg
     1381 aagggatctc cctatactgt cctgttaaga gccattagaa tggcgcccga tccagtacca
     1441 tcagcaggag cagcgccggt aggtacacaa gcgacgcgag cgccagtcgc ctcacgtctg
     1501 tcgtggatcg ccagaggata aaggcacacg cacaaacaag caggccaatg cccagcgtaa
     1561 gcgcgccaac gaaatagact gggccggcga atccgatgag ggtcggcagt aggctgacga
     1621 caagcaagac catgcagtcg cagacaatct ggcatccagt cagtttatcg tcgggatcga
     1681 tcatggggag aagtcggatc cctgcccgcc tataatcgtc cttgtacagc atggcgattg
     1741 ccagcgtatg gggaagctgc cagagaaaca tgatggcgaa caacacccac gcctcgattc
     1801 cgagccttcc cgtggctgcc gcccagccga taaccggcgg caatgcccca ggtactgctc
     1861 ctacaaccgt gcagaaagcg gtcctgcgct tgagcggcgt gtaaagaaac agataacttc
     1921 ccacgatagc cgctgccacc acaccactca gggcgttgac taagatagcc aggtacagca
     1981 ggcccgtgac tacgatcaac aatccgaaca ggagcgcctc tgtgggccgt agtcgcccag
     2041 ccggcaatgg ccggagccgt gtacgcacca tccgtgcgtc tatgtcccgc tcgaggaact
     2101 gattcagcgc aagggttcca cccgccgcga gcgccgtacc ggtgagcacc tgcagcaatg
     2161 ccgcatagtc tggcgtgccg ctcacaccga gataaaagcc tacgaatgtg gtaagcaaca
     2221 ccatcatcac gatgcgcggc ttcatcagta acacgaaatc tgccgctcgc ctgcctgcat
     2281 gtgtgtactc agcgcttaat gtttcagatg tgagcatcat acgggtaccc gctcggcgag
     2341 caatccctgg ctgggggcgg cctgccgcga cgccggcagc cgataggctc gcagcgtgag
     2401 cacgatacag actgccagca tcagcgctcc cgtgattcta tgagtggctg gaagcgccaa
     2461 ccgagtaaag gccgcgtagg ggatttcgac ggacgtaaag cgattgatat acgacccaag
     2521 acccaataga agctgcagca ccaacaggcc gatgagtaga g
//
LOCUS       DATMXI010000374         7505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_7173, whole genome shotgun sequence.
ACCESSION   DATMXI010000374 DATMXI010000000
VERSION     DATMXI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7173"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VN444_07190"
     CDS             <1..612
                     /locus_tag="VN444_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HWR21628.1"
                     /translation="LGGAGRISDVARELTEIERALVSKLLRRTLEDLRSAWSRVSLFQ
                     FELLNLEVNPGFLQLAAPTDMVILIGFDVKIGDVEGMMSLCFPFSMLEPVIPNLSLRR
                     WIAGHREETDEVGAAEIAKAMPAVGLSVRALLGSIPLTIHELSFLRTGDIVRLDMGAS
                     SLGVLEVEGVPKYVVKVGTSHRKRAVQIVADISEEKSAHGAHH"
     gene            596..1570
                     /gene="fliN"
                     /locus_tag="VN444_07195"
     CDS             596..1570
                     /gene="fliN"
                     /locus_tag="VN444_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018660071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HWR21629.1"
                     /translation="MAHTTDRRNQSASIAEVETASSALERHGQLVFEASSQVFSALCG
                     RPVRLRFDRIVPVSPSTLLALLPGPCVGMAMQASEGLFGAEHLVWRADDAIGLAQLIL
                     GEEPTPGSELSADYLDALSEAANQIGGSLGTALRAALGKPVTVEAGTVTALTEAGVCL
                     AVFREEASTPLLCVASLTREGSASGEVALIVSPSLLPETDKAQPHNDALVAKEDTVSA
                     TSASAQAPFVPLTGGERVGPNNGIDMLLDVNLQVSVELGRTRLQIRDILQLGPGSIVE
                     LDKQAGDAVDILVNDKPIAKGEVIIIDENFGVRLTSITSVADRIKNLR"
     gene            1774..2322
                     /gene="fliO"
                     /locus_tag="VN444_07200"
     CDS             1774..2322
                     /gene="fliO"
                     /locus_tag="VN444_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HWR21630.1"
                     /translation="MPCIAIAELPQTTGVSEPTPVSDSQRLDLPSYRDPEIPLPGAGV
                     TSQVFTALGTVLGVLALGVYLYKRFALRGPRGMNRDGTIRILSRTYLGPKESLCLVQV
                     GTDILLLGQTGSGITLLHTLPSNASAVSSDRGADDATIGGIGGSENCSPSGFARERAV
                     ALSGLEGRLRRLNKLWGTGASE"
     gene            2319..3050
                     /gene="fliP"
                     /locus_tag="VN444_07205"
     CDS             2319..3050
                     /gene="fliP"
                     /locus_tag="VN444_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744291.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HWR21631.1"
                     /translation="MMGIGTFVAASPLAVGPSFVPLPQLPLVAGGAGSAEPTVAVQLV
                     ILLTLLAMAPALVIMLTSFSRIIIVLSFLRTAMGTQQMPPNQVLIGLTLFLTVFIMAP
                     VGEQINREALQPYLSRKLAQETALERAVQPLREFMFKQTRERDLALMVHLARLERPKD
                     RAAVPTHVLVPAYAISELRTAFQIGFVLFIPFLVIDMVVSSVLISMGMLFLPPPVISF
                     PFKLLLFVLADGWHLVVRSLVMGFQ"
     gene            3064..3333
                     /gene="fliQ"
                     /locus_tag="VN444_07210"
     CDS             3064..3333
                     /gene="fliQ"
                     /locus_tag="VN444_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HWR21632.1"
                     /translation="MNPQVVLDLGSQALQLILLLSAPILVAGLIVGLLVSIFQAVTSI
                     QEATLAFVPKIVVVFVALILFLPWMMRTMLSFTTSLLINLPLYGR"
     gene            3388..4182
                     /gene="fliR"
                     /locus_tag="VN444_07215"
     CDS             3388..4182
                     /gene="fliR"
                     /locus_tag="VN444_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWR21633.1"
                     /translation="MVVLSAWSSPLFLLILFRLGGLLLSAPIFAGSHLPVSFKVGLAL
                     GLAVSLYPLLSKSTLAARGMEPFSLLLNIVGEVAVGVVIGLAARLLFVGVSLAGELAG
                     VQMGLGIASVVDPQFPQQTSVVAQFMELMTMLTFLTLQGHHAFLEAVWASFESIPLGM
                     FSGAAAAGAASVLVALFGKSLVLAVKLSSPIMVAVLITNVAIGLLARVVPQLNFFAFG
                     LALTFIVGLLTLLVSLPLLNHLIGISTGQVRLELFTLLRSLSHAAR"
     gene            4169..5248
                     /gene="flhB"
                     /locus_tag="VN444_07220"
     CDS             4169..5248
                     /gene="flhB"
                     /locus_tag="VN444_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HWR21634.1"
                     /translation="MPPDDARERTESATSKRRQEARGRGQIAKSQEINSALLLLSGTL
                     VFYLLGGSLVTGLVGLMRLYLGRGPGGELTLPQVHQLMISASSRMAMMLAPFMGTLVV
                     VAYVANVAQVGFLTNSSALSLKWERLNPTAGFRRLFAPGRMLAETVKSPLKFALVGFI
                     VYRTLSRHLSEIPLLADMAPIQMLTWFGQICFQLALRVSLVFLVLAAMDYGWQRWQFE
                     KSIRMTREEVKEEMKQTEGDPAVKGRMKSLQRQAAMHRMMTDVPKADVVIVNPIHFAV
                     AMRYDSTSMEAPTVVAKGARLVAERIVAVARAHGLPIVEDPPLARALYKAVSIGRPIP
                     IHLYRAVAEILAYVYQMSGKRKAAR"
     gene            5245..7338
                     /gene="flhA"
                     /locus_tag="VN444_07225"
     CDS             5245..7338
                     /gene="flhA"
                     /locus_tag="VN444_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWR21635.1"
                     /translation="MKTTRTTPLAPLLQGDVLVALTSIGILLAMLVPIPPLLLDLLLA
                     ANITIALLIFVISLYILRPLDLSVFPSLLLVLTLFRLSLNVASTRLVLLHGNEGAAAA
                     GRIINAFGSFVVGGSYIVGAVVFAILAVIQFMVITKGAGRIAEVSARFTLDAMPGKQL
                     SIDAELNAGLINEQEARARRLEISREADFYGAMDGASKFVRGDAIAGMIIIAINILGG
                     LAIGVLQNGMDLMGALQTYTLLTVGDGLVAQIPALTISTAAGIVMTRAASEAELSSTL
                     GRQILFYPKPLGITAGVLLFIGLIPGMPFFPFFMVAAVIGTVAYSRVKAGPSGGGRRV
                     GEEGEDVPLAPSGPEKVETLLPLDLLTLEVGYGLIPLVDASQGGDLLDRIKLIRRQFA
                     IDMGFVVPPVRIRDNLSLRPNSYSVKIKGVEVAQGEVLVGHFLAMDPGTAQEPVDGIE
                     TQEPTFGLPARWINAALKERAQVLGYTVVDPSSVVATHLSEVIRREARELVGRQEVQA
                     LLDHLKQTHPALVEGIIPTQLSLGAVQRVLQGLLMEGVSIRDLPTILEALVDYVPYTK
                     DPILLTEHARQALAKSICRSLMAPDGGLAVLTLGPSMEQTLAEAIIQNDQESYLALEP
                     RLAQKVVEAVAKGVEQCLPLLGQPVFLCTAAVRPHLRRLIERYLPQVTVLSYNEIGPR
                     VSVKVLRTVEISDAS"
BASE COUNT         1287 a         2130 c         2432 g         1656 t
ORIGIN      
        1 ctcgggggtg cgggacggat atccgatgtg gcgcgagaac tgactgagat tgagcgggcg
       61 ctggtgtcga agctgcttcg gcgcacgctc gaggacctcc ggagcgcctg gagccgcgtc
      121 agcctcttcc agtttgaact gctgaatctg gaggtcaacc cgggcttcct ccagcttgcc
      181 gcgcccaccg acatggtgat cctgatcggc tttgacgtca agataggcga cgtcgaagga
      241 atgatgagtc tctgctttcc gttctccatg cttgagcctg ttattccgaa tctgtcgctg
      301 agacgatgga tcgccgggca cagagaggaa acggatgagg ttggggctgc tgaaatcgcc
      361 aaggccatgc cggccgtcgg tctcagtgtc cgggcgttgc tcggttcgat cccgctgacc
      421 atccatgaac tgagctttct caggacgggc gatatcgtac gcctggatat gggagcctcg
      481 tctctcggcg tgctcgaggt agagggggtg ccgaaatacg tggtgaaggt cggtacgtcg
      541 cacaggaaaa gggctgtcca gattgtggcc gatatctcag aggagaagtc agcgcatggc
      601 gcacaccact gatcgacgca accagtcagc atctatagcc gaagttgaga cggcgtcttc
      661 agcattggag cggcacggac aacttgtctt tgaggcctcc tctcaggtct tctcggcact
      721 ctgtggccgc ccggtgcggc ttaggttcga tcggattgtt ccggtatcgc cctcgacatt
      781 gctcgctctc ctgcccgggc catgtgtcgg tatggcgatg caagcgagtg agggcctttt
      841 cggcgccgag cacttggttt ggagggcaga cgacgccatc ggcttggcac aactcattct
      901 gggagaggag cccacgccag gatcggagtt gtctgcggac tatctcgatg cgctctcgga
      961 agccgcgaat cagatcggcg gaagtctcgg gacagcgctg cgagcagcgt tggggaagcc
     1021 ggtaactgtc gaagcgggga ccgtgaccgc tttaaccgag gccggggtct gccttgcggt
     1081 attccgcgaa gaggcgtcta ccccgttgct ctgcgtagcc agtctcacac gtgaaggctc
     1141 ggcgtctggg gaggtggcgc tgatcgtctc tccgtctctg ctgccggaaa ccgataaggc
     1201 tcagccccat aacgacgctt tggttgcgaa ggaggataca gtgtctgcaa cgtctgcctc
     1261 agctcaagca ccattcgttc ccctgaccgg aggggaacgg gtcggaccca acaacggtat
     1321 cgatatgctg ctcgacgtca atcttcaggt aagcgtggaa ctcgggcgga cccggcttca
     1381 aattcgagat atcctccagc tcgggccggg atcgatcgtc gagctggaca agcaggccgg
     1441 tgacgcggtg gacatccttg tgaacgacaa gccgatcgcc aagggtgagg tgatcatcat
     1501 cgacgaaaac ttcggggtga gactgaccag catcaccagt gtagccgatc ggattaagaa
     1561 tctgcgatga gtacgtggtg cgaagtacga gccctgtggg tcacaggtgg acggcgattg
     1621 actccgctta atctcgccgt acgtccatgt acggctcgaa tcggcgccgg caggctgtgc
     1681 tgtccatgct tcgcctgcca tcgacgacgc gtcgccaacc cccgtccact ctggcgtgcg
     1741 caccttgcca ttatgctctg gctcgttggg gcaatgccat gcatagcgat cgccgaattg
     1801 ccgcaaacga cgggcgtaag tgagccgacg cctgtctccg attctcagcg gctcgatttg
     1861 ccgagttatc gcgatcccga aatcccgtta cccggcgccg gcgtcacctc gcaggtcttc
     1921 accgcgctcg ggacggtgct tggcgtgctg gcgcttgggg tctacctcta taaacgattc
     1981 gcattgcgcg ggccgcgggg tatgaaccgg gacgggacca tccggatcct gagtcggacg
     2041 tacctgggcc cgaaggaatc gctgtgtctt gtgcaggtgg ggaccgatat cctgctgctt
     2101 ggccagacgg gttccgggat tacgctgctg cacacgcttc cgtcgaacgc ctccgcggtg
     2161 tcctccgaca gaggagccga tgacgcgacg atcggcggga ttggcggatc tgagaattgt
     2221 tcgccgtcgg gatttgcgcg ggagcgcgcg gtggcgctga gtgggctgga gggtcggttg
     2281 agacgactga acaagctctg gggaacgggg gcctcggaat gatggggatc ggaacatttg
     2341 tcgcggcgtc gccgctcgcc gtagggccat cgtttgtccc ccttccgcaa ctgccgttgg
     2401 tcgcgggcgg ggcaggaagc gcagagccga cggtagcggt gcagctcgtt atcctgctga
     2461 cgctattggc catggcgccc gcgctcgtga tcatgctgac ctctttctcc aggatcatca
     2521 tcgtcctgtc cttcctgcga acggccatgg ggacccagca aatgccgccc aaccaggtct
     2581 tgatcgggct tacactgttc ctcaccgtgt ttattatggc gccggttggc gagcaaatca
     2641 accgggaggc cctccaaccc tacctgagcc ggaagcttgc ccaggagacg gccctcgaac
     2701 gcgccgtgca gccgcttcgg gagttcatgt ttaagcagac gcgggagcgg gacctggccc
     2761 ttatggtcca cctggcacgg ctggagcgac cgaaagaccg cgccgcggtc ccgacccacg
     2821 tgctggttcc ggcctacgcc atcagcgagt tgcggaccgc ctttcagatc ggatttgtcc
     2881 tgttcatccc ctttctcgtg atcgacatgg ttgtctcgag cgtgctcatc tcgatgggca
     2941 tgctgtttct gccgcctccg gtcatctcgt ttccattcaa gttgttgctg ttcgtcctcg
     3001 ctgatgggtg gcatctggtg gtgcgctcgt tggtgatggg gttccagtga ggcggaggat
     3061 cgtatgaacc cacaggtcgt gctcgatcta ggctcgcagg ctctgcagct catcctgctt
     3121 ctatcggcgc ctattctcgt tgccggtctt atcgtagggt tgctggtcag catttttcag
     3181 gcggtgacct cgatccagga ggcaacgctt gcctttgtcc cgaagattgt ggtggtgttt
     3241 gtggcgctga tactcttttt gccctggatg atgagaacga tgctgtcgtt caccacatcg
     3301 ctgctcatca acttgccgtt gtacgggcgg taacgacctc atggaacgga cgcggttgta
     3361 cgcggccttg ggagcgtgac tgtggatatg gtggtgctgt ccgcctggag ttcgccgttg
     3421 tttctgctga tcctctttcg tcttgggggc ctgcttctgt ccgcgccgat ctttgcgggc
     3481 agccaccttc ctgtctcctt caaagtgggc ctggcgctcg gtctcgccgt aagcctctat
     3541 ccattgctta gtaagagcac cctggctgcg cgcggtatgg agccgttttc gttgctgttg
     3601 aacatcgtag gagaggtagc ggtaggggtg gtgatcgggc tggcggcgcg actgctgttc
     3661 gtcggggtga gtcttgcggg ggaacttgcc ggagtgcaga tgggcctcgg gatcgctagt
     3721 gtcgttgatc cgcagttccc acagcagact tcggtggtcg cgcaattcat ggagctcatg
     3781 accatgctca cgtttcttac gcttcaggga caccacgcct ttctggaggc ggtctgggcg
     3841 agctttgaat cgatccccct cggaatgttc tccggggcgg cggctgcggg cgcagcctcg
     3901 gtcctggtcg ccctcttcgg taagagctta gtcctggccg tcaaactctc ctccccgatc
     3961 atggtggcgg tactgatcac gaatgtcgcc atcggtctgc tggcgcgggt ggtgccacaa
     4021 ctgaattttt tcgccttcgg cctcgcgttg acttttatcg tcggcttgct gaccctgctg
     4081 gtctctttgc cgctccttaa ccatctgatc gggatcagta ccggccaggt ccgcctggaa
     4141 ctgtttacgc tactacggag cctctcacat gccgccagat gacgccaggg aacgaaccga
     4201 atccgcgacg agtaagcggc gccaggaggc cagaggacgg ggccagatcg ccaaaagtca
     4261 ggagatcaac tcggcgctgc tgctgctcag cgggaccctg gtgttctacc tgctcggcgg
     4321 ctcactggtc accggcctgg tggggcttat gcgtctttac cttgggcggg gccccggcgg
     4381 tgagctgacg ctcccgcagg ttcatcagtt gatgatcagc gcctcctccc gaatggcgat
     4441 gatgctcgcg ccgttcatgg gtacgttggt tgtcgttgcc tatgtcgcga atgtcgcgca
     4501 ggtcggcttc ctgacaaact cgagcgcctt gtcgctgaag tgggaacggt tgaatccgac
     4561 ggccgggttc cggaggctgt tcgcgccggg acggatgctt gcagagacgg tcaagtcccc
     4621 cctgaaattc gccctggtcg gctttatcgt ctatcggacg ctcagccgcc atctatctga
     4681 gattcccctc ctcgcagata tggcgccgat tcaaatgctc acctggttcg gccagatctg
     4741 ttttcagctt gcgctgcgag taagtctggt cttcctggtg ctggccgcca tggattatgg
     4801 ctggcaacgg tggcagttcg agaaatcgat ccggatgacg cgggaagagg tgaaggagga
     4861 gatgaagcag accgaaggcg accctgccgt caaggggcgg atgaagagcc tgcagcggca
     4921 ggcggccatg caccggatga tgacggatgt cccgaaggcg gatgtggtaa tagttaaccc
     4981 gatccacttc gccgtcgcca tgcggtacga ttcgacctcg atggaggccc cgacggtcgt
     5041 ggcgaaaggg gccaggctgg tggctgaacg gattgtggcg gtggccaggg ctcacgggct
     5101 tccgatcgta gaggaccccc ccttggcccg tgctctctat aaagccgtct ccataggccg
     5161 tccgattccg atccacctgt atagggcggt ggcggagatc ctggcttacg tctatcagat
     5221 gagcgggaaa cgaaaggcag ctcgatgaaa acgacccgga ctacgcctct tgcgccattg
     5281 ctgcagggtg atgtcctggt ggcgctgacc agcattggca tcctcctggc aatgttggta
     5341 ccgatcccgc cgctgctcct cgacctgttg ctcgccgcca acatcacgat cgcgctcttg
     5401 atctttgtga tctcactgta catccttcgc cctctcgatc tctcggtctt tccatcgctg
     5461 ctgttggtgc tgacgttgtt ccgcctttcg ttgaacgtgg cctcaacgcg cctggtcctg
     5521 ttgcatggca atgaaggcgc tgcagcggca ggtcgcatca tcaatgcatt cggaagtttc
     5581 gtggtgggcg gcagctacat tgtgggcgct gtcgtatttg ccatcctggc ggtgatccag
     5641 tttatggtca ttaccaaggg cgcagggaga attgccgagg tgtccgcccg ctttaccctc
     5701 gatgctatgc ccgggaaaca actctcaatc gatgccgagc tgaacgccgg actcatcaac
     5761 gagcaggagg ccagagcccg caggctggag atatcgagag aagccgactt ctatggggca
     5821 atggacggcg ccagcaagtt cgtccgggga gatgcgatcg ccggcatgat cattatcgcc
     5881 atcaacattc tgggaggcct cgctatcggt gtccttcaaa acgggatgga tctgatgggg
     5941 gcgctacaga cctatacgct gcttactgtt ggtgacggtc tggtggcgca gattccggcc
     6001 ctgactattt ctacagcggc cggtatcgtg atgacgaggg ccgcctctga agcggagctt
     6061 agttccactc ttggccgcca gatactcttt tacccgaaac cgctcggcat tacggcgggc
     6121 gtactcctgt ttatcgggct gattcctggc atgccgttct ttccgttctt catggtagcg
     6181 gccgtcatcg gtacggtggc atattccagg gtcaaggccg ggccgtcagg aggtgggcga
     6241 agggtcgggg aggagggaga ggatgttccg ttggcgccct ctggtcccga aaaggtggag
     6301 acgctcctgc cgctggatct gttgacgttg gaggtagggt acggtctgat cccgctggtg
     6361 gacgcaagcc agggtgggga cctcctggat cggatcaagc tgatccgacg ccaattcgcc
     6421 atcgatatgg ggtttgtggt cccgccagtc agaatccggg ataacctctc gctcaggccc
     6481 aacagctaca gtgtcaagat taaaggcgtg gaggtcgccc agggagaggt cctggtgggg
     6541 cacttcctgg ccatggatcc ggggacggcc caggagccgg tggatgggat tgagacccag
     6601 gagccgactt ttggcctgcc ggcaaggtgg atcaatgcgg cgttgaagga gcgcgcgcag
     6661 gttcttggct acaccgtcgt ggatccctcg tcggtcgtgg ctacccatct gtcggaggtc
     6721 atacggcgag aggcgcggga gctggtgggt cggcaggagg tgcaggcgct cctcgaccac
     6781 ctgaagcaaa cccatcccgc gttggtagag gggatcattc ctactcagct ctctttgggc
     6841 gcagtacagc gggttttgca ggggctgctg atggaggggg tctcgatccg ggacctcccg
     6901 acgatcctcg aggcgctggt cgattatgtg ccttatacca aagatccgat cttgctgact
     6961 gagcacgcgc ggcaggcgct ggccaaatcg atctgccgat cgctcatggc gcccgatgga
     7021 ggactggcgg tgctgacgct cggaccgtct atggaacaga cgttggccga agcgatcata
     7081 cagaatgatc aggagagcta cctggcgctg gagcccaggc ttgcgcagaa ggtggtcgaa
     7141 gccgttgcga agggggtgga gcagtgtctg ccgctcttgg gacagccggt cttcctctgc
     7201 accgctgcgg tccgcccgca tctgcgacgc ctgatcgaac ggtatctgcc gcaggtgacg
     7261 gtgctgtcgt ataacgagat cggacctcgc gtgtcggtga aggttctgag gacggtggag
     7321 atcagcgatg catcttaagc gattccgcgc ggcgaccatg gatgacgcgt tgcggcagat
     7381 cggggaagag ttaggtcctg aggccgtgat tctgcacacc aagagtattc cgctcgccgg
     7441 tgccatgccg tttgcgaagc gacagggcgt agaggtcgtg gcagcgctgg acggtgatcc
     7501 gtccg
//
LOCUS       DATMXI010000375         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_69215, whole genome shotgun sequence.
ACCESSION   DATMXI010000375 DATMXI010000000
VERSION     DATMXI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69215"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VN444_07230"
     CDS             complement(<1..654)
                     /locus_tag="VN444_07230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWR21636.1"
                     /translation="MEQFYNAFETWGLPLFVPQLIVMVVVATAVLIFVAVSVMFMVWW
                     ERKISAHIQVRFGPMRVGGWHGWAQSIADGIKLLIKEDIVPEGADRLVFALAPMVVFA
                     AGLAAYVIIPFGPGLIVSDLNIGVLFYISISSLTVVGIIMAGWSSNNKYSVLGAIRSA
                     AQAVSYEVPLVVSILGVIMTVGSLSMTRIVEAQQGVWFVIPQLLGFLIFLTAAIAECN
                     "
     gene            complement(683..1817)
                     /locus_tag="VN444_07235"
                     /pseudo
     CDS             complement(683..1817)
                     /locus_tag="VN444_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            complement(1843..2307)
                     /locus_tag="VN444_07240"
     CDS             complement(1843..2307)
                     /locus_tag="VN444_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWR21637.1"
                     /translation="MTPDEVTEAIKSQFGDAVKASEIKGVEARMDIHQEKSYEILAAL
                     KGMGFDYLNCLSAVDRIASGELEVVYNLSSLSQPTKILVRVRVPREDPIIRSVVPLWS
                     TADWHEREAFDMMGIRFDGHPDLRRILTSEDWVGYPLRKDYQDERLVPYEPV"
     gene            complement(2307..2666)
                     /locus_tag="VN444_07245"
     CDS             complement(2307..2666)
                     /locus_tag="VN444_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWR21638.1"
                     /translation="MAADYAVVGVFLVVGLLFVVLNVDVVSRLLRPSNPQREKLTTYE
                     CGEDPVGASWIRVHVRYYLYALVYVIFAVETIYLLPWAVVFRKLGMFAFVEMMLFIAI
                     LLVGFAYAWRKGALEWV"
     gene            complement(2893..>3172)
                     /locus_tag="VN444_07250"
     CDS             complement(2893..>3172)
                     /locus_tag="VN444_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWR21639.1"
                     /translation="YGLRCASCHGGSGKGDGWRAKLSWLKMPDFSDSAYMQTRSDDDL
                     LLAIKQGRKSVMPSYGLEMTDREIKDLVVYIRSFDKAPAPPKPMGTAR"
BASE COUNT          736 a          976 c          843 g          617 t
ORIGIN      
        1 gttgcattcc gcgatggccg cggtcagaaa gatgaggaag ccaagcaatt gcggaatcac
       61 aaaccagacc ccctgctgcg cctctacgat ccgggtcatg ctcagggagc cgaccgtcat
      121 gatgacccca aggatcgaga ccacgagggg gacctcgtag cttaccgcct gcgccgccga
      181 gcggatcgcg cccaggactg aatacttgtt gttggagctc cagccggcca tgatgatccc
      241 caccaccgtc agggaggaga tcgaaatata gaagagcacg ccgatgttca gatcgctgac
      301 gatgaggccc ggaccaaacg ggatgatgac ataggcggca agcccggccg caaagaccac
      361 catgggcgcc agggcaaaca caagccggtc agcgccctcc gggacgatat cctccttaat
      421 gaggagcttg attccgtcgg cgatactctg ggcccagcca tgccagccac cgacccgcat
      481 cggcccaaag cgaacctgga tgtgggcgct gatcttccgc tcccaccaaa ccatgaacat
      541 gacggagacg gcaacgaaaa tgagaacggc cgtggccacc accaccatca cgatcaactg
      601 agggacgaac aggggcaacc cccaggtctc gaaggcgttg tagaactgct ccatcagcgt
      661 ctcactgctc acggtcgcca cttcaccgat ctacctcgcc cagtacgatg tcgatgctcc
      721 ccaggatggc cacaatatca gcgacgaggc agcctcgacc aatgacggga agtacactca
      781 ggttgacaaa ggccggggag cggaccttgt accggtacgg tttctcagag ccgtcggaaa
      841 cgaggtagaa ccccagctca cccctgggcg actctaccct ggtgtagatg tctccgacgg
      901 gcggcttcag ctttttcggc agtttcccga taatctcacc gggcggcaga ccctgaagag
      961 cctgctcaac gatctttacg ctctcgtcca tctcgatacg tctgaccata tatcggtccc
     1021 agacatcgcc ggaggttccc accgggatgt cgaattcgaa gcgatcgtaa atggagtacg
     1081 gatcgttccg gcgtagatcc cacttaatcc cggaggcacg gagcatcggc ccgctgatgg
     1141 cgtaatcgat ggcggtcttt gcgtcgagtt tcccgagacc ctgtacccgc ttctggaaga
     1201 tgatgttgtc gctcatgagc gcgtcgtact ctggcagacg cggtttaaac cattccagaa
     1261 atgccttgca atcagccagg aaggaatcag ggaagtctaa cggcatcccg ccgatccggt
     1321 agaaagcata gttcagccgc tgccccgaaa ccgactcgaa gagatccagg atcttctccc
     1381 gctcccggaa ggcgtacatg aaaatcgtga agttacccag gtcgaggcct aacgtcccca
     1441 accagatcag gtggctggcg atccgttgca actcccccag gatcacccgc atatactcgg
     1501 ctcgctccgg cacaacaatc ccgatgagcc gttccaccgt tagcacatag ccaaagttgt
     1561 tatacatgga ggcgagatag tccagccgat cggtatacgg aaatgatttg ggtgaagatg
     1621 cggttctcgc cgatcttttc atgacagcgg tgcaggtagc cgatgtacgg gatgacctcg
     1681 gtcacgacct caccatccag cttcaacagg aggcggagca cgccgtgcgt actggggtgc
     1741 tgcggcccca tgttgacgta catctcctcc atcgtctcct gggtgtcttc agcgatctcc
     1801 atctcttgcg gactcatctg cctgcctcaa ctgaaaatgt cactagaccg gctcgtacgg
     1861 aaccagccgt tcgtcttgat aatccttgcg caacggatat cccacccagt cctcagaagt
     1921 caggatccgc cggaggtcag gatgaccatc gaagcggatc cccatcatgt cgaatgcctc
     1981 ccgttcgtgc cagtctgccg tgctccacag cggcaccacg ctccggataa tcggatcctc
     2041 ccgcggaacc cgtaccctca ccaggatctt cgttggttgc gacagggagg agaggttata
     2101 gaccacttcg agttcgccgc ttgcgatccg atccacagca ctcaggcagt tcagatagtc
     2161 gaaccccatc cccttgagcg cggcgaggat ctcatagctc ttctcctgat ggatatccat
     2221 tcgggcctcg acacccttga tctctgaagc cttgacggcg tcgccgaact gcgacttgat
     2281 ggcttcagtg acctcgtccg gcgtcatcag acccactcca gggcgccctt ccgccaggcg
     2341 taggcgaaac cgaccagcag aatggcgata aagagcatca tctccacaaa ggcaaacatc
     2401 cccagcttcc ggaagaccac tgcccaagga agcagataga tcgtttctac cgcgaagatc
     2461 acatagacca aggcgtacag gtagtaccgg acgtgaacac ggatccagga ggcgccgacc
     2521 gggtcctccc cgcattcata tgtggtcaac ttttcgcgtt gaggattact gggccgaagc
     2581 agtcgggaaa cgacgtcaac attcagcaca acgaagagca gcccgacgac cagaaatact
     2641 cctacaactg cataatctgc tgccatctca ccacctagtg cgcgtccgat atggacgaga
     2701 catgcgttac ggcttggcct cgtgaagggg atcgccgggg cctaagttaa cggcgacaaa
     2761 aaaaaagccc cgcgcagggc atcctgaaga gtttgccgac tgttcgctgc agggctttcc
     2821 gagattccac gattaagttc gtgaaacttt tagcatatcc tccttcacga tgtcaagggg
     2881 aaaagcgcat agttaacgcg ctgttcccat tggcttcggc ggcgccggcg ccttgtcaaa
     2941 gctcctgatg tagaccacca gatccttgat ctcgcgatcg gtcatctcaa gcccatatga
     3001 gggcattacc gacttacgac cctgcttgat agctagcagg aggtcgtcat cggatcgtgt
     3061 ttgcatatag gccgaatcgg aaaagtctgg catcttgagc caagaaagct ttgccctcca
     3121 tccatcgcct ttcccgcttc caccgtgaca tgaggcacac ctaaggccgt aa
//
LOCUS       DATMXI010000376         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_313433, whole genome shotgun sequence.
ACCESSION   DATMXI010000376 DATMXI010000000
VERSION     DATMXI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313433"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..965
                     /locus_tag="VN444_07255"
     CDS             51..965
                     /locus_tag="VN444_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21640.1"
                     /translation="MMPYLWGIYREQQHSPGREFDDAEILRLVAKHVEAAGYQVRLKT
                     PEQLGAADPCLKPAGTDDPSTGLRPDPLPSLVFLMCERAETLSSLEPIEMQAIPHVNP
                     LQAVLNTYRDRMIPLLAASAIPIPRSQIISSNSSDPSHPSPQLSTGPFPIWIKRADVH
                     NTQSGDVELVSSPEDLRVALVALHGRGISHVVLQQHIPGDLIKFYGIGRPLAGRWKDA
                     WFKWFYHRDQELARYPFSEEALQTLTQRAASTIGLEVYGGDAIITPAGEIFLIDINAW
                     PSFALFREEAAPQIATWLLHRLRQGALL"
     gene            962..2287
                     /locus_tag="VN444_07260"
     CDS             962..2287
                     /locus_tag="VN444_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWR21641.1"
                     /translation="MKRNIPGKRSKVIVTRETRHMAPGLQSIALYSGLAMASGQGCTL
                     IDEDGNRYLDFMAGIGVGSIGHTHPHYVEAMKAQIERLTFGSFTTENRARFLALLASV
                     TPKGLKRIQMYSSGAEAVEAAFRLAKSATQKFEFVGFWGGFHGKTGGVLGLLGDDFKK
                     QMGPFMPGLYSAPYANCYRCPLKLRYPDCGIACADFLRQVIQCQTQGEIAAIIVEPIQ
                     GTAGNVIPPPGFLSAVQAIAKECGALLIADEMLTGFGRTGSMWGCDHEPIVPDVMTVG
                     KGMGGGFPVSGVISTQELMASKPFGNPSGSSSSYGGNPLAATAGLATLEVIVKDRLVA
                     NAKHVGAVMLKRLEAMKERYPFVGDVRGRGLLLGIELVKDRQTKEPLSKPITQRLFQA
                     CLRRGLLAMCYSYNIRINPPLLVTEAQALEGLAILDEAFAELEKTGYRG"
     gene            2366..>3243
                     /locus_tag="VN444_07265"
     CDS             2366..>3243
                     /locus_tag="VN444_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR21642.1"
                     /translation="MMLRGAIIGLGNVAVNGHLPGWEGCERAQIVAAADALESRLVEA
                     RWRLPRARLYTEVEALLTSEPLDFLDICTPPGTHASLTRMALHRGLHVLCEKPLVLSP
                     DELRLVRRAQVESGRVLHTVHNWLYAPIIKKATDLILEGQIGTVRYINWQVLRREPSI
                     SVGPVLGSVEACPEPSRGGAAHDPRSASKIGNWRLDPAMAGGGILLDHGWHALYIILR
                     WLCQDPRTISATLEKRAHPEWPVEDTATVQINFPEAVADIFLTWDASSRGNSAVIEGT
                     CGAIRVDDDILLLTGK"
BASE COUNT          647 a          917 c         1014 g          665 t
ORIGIN      
        1 ccctaaaccc tcgaccctaa accctgccta aaaagcggag acagcacctc atgatgccgt
       61 acctttgggg aatttatcgt gaacagcagc actcgccggg ccgggaattc gatgatgctg
      121 agatcctgag gctcgtggcc aaacatgtcg aagcggctgg ctaccaggta cgcctcaaga
      181 cgccggagca actcggagcg gccgacccct gcttaaaacc tgccgggaca gacgaccctt
      241 cgactgggct caggccagac cctttgccgt cgcttgtatt tttaatgtgc gagcgggcag
      301 aaaccctgag ttcccttgag ccgattgaga tgcaagccat cccacatgtc aatcctctgc
      361 aggccgtgct gaacacctat cgggaccgaa tgatcccgct cctcgccgcc tcagcgattc
      421 cgatcccaag gagccagatc atttcgagta actcgtccga ccctagtcat ccttcgccac
      481 agctttcgac aggccctttt ccgatctgga tcaagcgagc cgatgtccac aatacccaat
      541 ccggggatgt cgagctcgtg tcgtctccgg aggatctccg ggtggctctg gtagcgctcc
      601 acggcagggg aatctcccac gtggtactgc agcagcacat tccgggagat ttgatcaagt
      661 tctacgggat cgggaggcca cttgcgggcc gttggaagga cgcatggttt aagtggttct
      721 accaccggga tcaggagctg gctcggtatc ctttttcgga ggaggcatta cagaccctca
      781 cccagcgggc ggcttccacc atcggtcttg aggtgtacgg aggtgatgcc atcatcacac
      841 ctgccgggga gatcttcctc atcgacatca atgcctggcc ttcatttgcg ttgtttcgcg
      901 aggaagctgc tcctcagatc gccacgtggc tgttgcatcg cctcagacag ggagcactcc
      961 tatgaagcga aatattcctg gaaagcggtc gaaggtgatc gtcacccgtg agacacgcca
     1021 catggcgccg ggcttacagt cgattgcgct gtattcgggg ctggcgatgg ccagcggtca
     1081 agggtgtacg ctgatcgatg aggacggcaa ccggtatctc gattttatgg ctgggatcgg
     1141 tgtggggagc atcgggcata cgcaccctca ttacgtcgag gcgatgaagg cccagatcga
     1201 gcggctcacg ttcggtagct tcaccaccga gaaccgggcc cggtttcttg cgctgttagc
     1261 atcggtcacg ccgaaagggc tcaagcggat ccagatgtat tccagcgggg cggaggcggt
     1321 cgaggctgcc ttccgactcg ctaagtcggc gacgcagaag tttgagtttg tcgggttctg
     1381 gggcgggttc cacggtaaga ccggcggggt ccttgggctg ctgggggatg actttaagaa
     1441 gcagatgggg cctttcatgc ctggtctcta cagcgccccg tatgccaact gttatcgctg
     1501 tcccttgaag ctccgttatc ccgactgcgg tattgcctgc gcagatttcc tgcgccaggt
     1561 gatccagtgc cagacccagg gcgagatcgc cgcgatcatc gtggaaccga tccaggggac
     1621 tgccggaaat gtgattccgc ccccagggtt cttgagcgcg gtgcaggcga ttgccaaaga
     1681 gtgtggcgcc ctgctgatcg ccgacgagat gctcacaggc ttcggccgca ccggatcgat
     1741 gtgggggtgc gatcacgagc cgattgtacc ggacgtgatg acggtcggta aggggatggg
     1801 cggtgggttc cctgtcagcg gggtaatttc gacacaagag ctgatggcga gcaagccctt
     1861 tggcaatccg agtggaagct cctcaagcta tggaggcaat ccgctcgccg caacagcggg
     1921 gctggctacc ctcgaggtga tcgttaagga tcggcttgtg gcgaatgcca agcatgtcgg
     1981 tgcggtcatg ctgaaacggc tggaagcgat gaaggagagg tacccgtttg tgggcgacgt
     2041 gcggggcaga gggctcttgc tggggatcga actggtcaag gataggcaga ccaaagagcc
     2101 gctctcgaag ccaattacgc aaaggttgtt tcaggcctgc ctccggaggg ggctgctcgc
     2161 catgtgctat tcctacaata tccggatcaa tccgccgctt ctcgtcaccg aggcgcaggc
     2221 attggaaggc ttggctatcc tcgatgaggc ctttgccgag ctagagaaga cagggtatag
     2281 gggatagggt ggagggtgta aaggaaaggc cagggctttg tccaacccct ataccctatc
     2341 ccctataccc taaaccctgc atttgatgat gttgagaggc gccatcatcg gtctgggaaa
     2401 tgtggcggta aatgggcatc tgccaggatg ggaggggtgc gagcgggccc agatcgttgc
     2461 cgccgctgat gccctcgaat cgcggctcgt tgaggcccga tggcgtcttc caagggccag
     2521 gctctacacc gaggtcgagg cgctgcttac ctccgagccc ctcgacttcc tcgatatctg
     2581 cacgccccct ggaacccacg catctctcac ccggatggcg ctgcaccggg gactccatgt
     2641 cttatgtgag aagcctctcg tgctctctcc cgatgagctg cgactcgtga ggcgtgctca
     2701 agtagaaagt gggcgagtcc tgcataccgt ccataactgg ctgtacgcgc cgattatcaa
     2761 aaaggcaacg gatttaatcc tggaggggca gatcgggaca gtgcggtaca taaactggca
     2821 ggtgctccgt cgtgaaccgt caatctcggt tgggcctgtc ctgggctccg tcgaagcctg
     2881 cccggagcca agtagagggg gagcagcgca cgatccccga tcggcgtcga agataggcaa
     2941 ctggaggctc gatccggcga tggcgggtgg cggaatcctg cttgaccacg gttggcacgc
     3001 cctgtacatc atcctgcgct ggctgtgtca agacccgagg acgatctccg ccacgctcga
     3061 gaagcgagcg catccagaat ggcctgttga ggatacggcg accgtccaga taaactttcc
     3121 cgaggcagta gccgatatct ttctgacatg ggatgcgtct tcacggggca atagcgccgt
     3181 gatcgagggg acctgtgggg ccatccgggt cgatgacgac atcctgctcc tgacaggtaa
     3241 gag
//
LOCUS       DATMXI010000377         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_182631, whole genome shotgun sequence.
ACCESSION   DATMXI010000377 DATMXI010000000
VERSION     DATMXI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182631"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..925
                     /gene="tsf"
                     /locus_tag="VN444_07270"
     CDS             308..925
                     /gene="tsf"
                     /locus_tag="VN444_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWR21643.1"
                     /translation="MSATIPATLVRELRERTGVGFMECKTALAESDGDLGRATTLLRE
                     KGLASASKKMGRTASDGLVISYIHGGGKIGVLLELNCETDFVARTDEFVSLARDVAMQ
                     VAATNPLYIRREEVPAELLEKERGILLAQVKSSGKPEKILEQIVQGRLEKFFSEVCLQ
                     EQPFIKAPEVKVEDRIKEVIAKVGENVVIRRFSRYQLGEKVECEA"
     gene            970..1698
                     /gene="pyrH"
                     /locus_tag="VN444_07275"
     CDS             970..1698
                     /gene="pyrH"
                     /locus_tag="VN444_07275"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWR21644.1"
                     /translation="METLKYKRIMLKVSGEALAGDEKFGISPRVLSFLADEIQEIHVL
                     GVQVAVVVGGGNIFRGVAASAEGMDRSSGDYIGMLATVINGLAMQDVLERKGIPTRVQ
                     TAIEMRELAEPFIRRRAVRHLEKGRIVIFVGGTGNPYFSTDTAAALRAMEVGAEVVFK
                     ATRVDGVYSADPLLDPSAKKFDELSYSEVLNRQLKVMDSTAISLCMDNLFPIVVFNLR
                     QPGVLRQLVFGEKVGTIVRGSEKC"
     gene            1692..2249
                     /gene="frr"
                     /locus_tag="VN444_07280"
     CDS             1692..2249
                     /gene="frr"
                     /locus_tag="VN444_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWR21645.1"
                     /translation="MLKEIQTKAEKEMRKAIDATLKGFNGVRTGRASIALLDGLMVEA
                     YGSAAPLNQVATMAVPELRMITVQPWDPSLLTAIERAILKSDLGVTPGNDGKIIRIAI
                     PSLTEERRKDLVKVVRKIAEEGRVAVRNARREANESLKRQEREKETSADEIKHGADMI
                     QDLTNRLIHEIDGLMAKKEKEIMEF"
     gene            2345..3229
                     /locus_tag="VN444_07285"
     CDS             2345..3229
                     /locus_tag="VN444_07285"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWR21646.1"
                     /translation="MGIEKIDIFPFVLSPSKHNKGFAGQAHMHLKRILSAVVLLPAFL
                     LLVQFGTPFHFFLLVTLAILVGLHEFYGMAKAGGWHPLTPLGMGSGLALSCMEFLGAP
                     APWLISAVAGVVVLLLISLLVGGTEPKEAASRGAITLLGLIYVVGLLSFPALLRAMVL
                     GRIYIFYLVFVTWAGDTGAFYVGSTMGKRLLCPSISPRKTVEGSVGGLMCSVLASGLA
                     KFWFWEELGTVECLVMGIGLGVMGQIGDLCESMLKRGFGVKDTGALIPGHGGVLDRVD
                     SLLFTGPVLYVAALAGWV"
BASE COUNT          667 a          770 c         1079 g          717 t
ORIGIN      
        1 attcgagcca ttcgactgat ggtggcccgc atggctgacg ctatccagga gattcgggcc
       61 atacggctga aggctactga ggacgtagct attgaggaag ctcagctcgc cccttcgtca
      121 gaagaagcga gttcgagtga ggtgccactg gaggttcttc aacagctcta agatcgggcg
      181 aagcgattag ctttaggcgg aagaggccga aggtagatag gctgaaggtt tttaggattc
      241 ttcagtcttc agcctgaata cctgctttat gattactgat agctgttttc caagaaagag
      301 ggtgaggatg tcagcgacaa ttccggcgac gttggtaaga gagttacgtg aaagaactgg
      361 tgtggggttc atggagtgta agactgcgtt ggccgagtca gacggggatc tggggagagc
      421 cacgacacta ctccgcgaaa agggattggc ctcggcctcg aagaagatgg ggcgaactgc
      481 ctctgatggc ctggtgattt cgtatattca cggcggcggg aagatcggtg tgctgttgga
      541 actgaattgt gaaactgact ttgtagccag gactgacgag ttcgtgtcgc tcgccagaga
      601 cgtggcgatg caggtggcag cgacaaaccc attgtatatc cgtcgggagg aggtgccggc
      661 tgaactcctt gagaaagagc gagggatcct tttggcgcag gtgaaatcat ccggcaaacc
      721 cgaaaagatc cttgaacaga tcgtccaggg gcggctggaa aaattcttca gtgaggtctg
      781 ccttcaggag cagcctttca ttaaggcccc tgaggtcaag gtagaggatc ggattaagga
      841 agtgatcgca aaggtcggag agaatgtggt catccggcgt ttcagcaggt atcaattagg
      901 cgagaaggtg gagtgcgaag cgtaaccgga tcgcacgtgg cctcagtagg ctgccgtgaa
      961 gatggcacta tggagacgtt gaagtataag cggatcatgc taaaggtcag cggtgaggcg
     1021 cttgccggtg atgagaagtt tgggattagt ccccgcgtgt tgagcttcct ggccgatgaa
     1081 atccaggaaa tccacgtgct tggggtgcag gttgcagtcg tggtgggtgg cggcaacatc
     1141 tttcgagggg ttgccgccag tgccgagggg atggatcgtt cttccggtga ctacatcggg
     1201 atgctggcaa cggtcattaa cggcctcgca atgcaggacg tactggagcg gaaggggatt
     1261 cctacccggg tgcagactgc cattgagatg cgggagctgg cggagccctt catccgccgc
     1321 cgggccgtcc ggcatctgga aaaggggcgg atcgtgatct ttgttggagg gaccggtaac
     1381 ccgtatttca gcactgatac cgcggcggcg ctacgagcga tggaggtggg ggccgaggtg
     1441 gtgttcaagg ccacgcgggt ggacggtgtc tacagtgcgg atcccctact tgaccccagc
     1501 gccaaaaagt tcgatgagct ttcctatagc gaggtgctga atcgccagtt aaaagtgatg
     1561 gactcgacgg cgatctcctt gtgcatggat aatctctttc cgattgtcgt attcaacctt
     1621 cgccagcctg gggtcctccg ccagttggtc tttggtgaaa aggtagggac gatcgttcgt
     1681 gggagcgaga agtgctgaaa gagatccaaa ccaaggccga gaaggagatg cggaaggcga
     1741 tagacgccac gctgaagggc tttaacggcg ttcgaaccgg gcgggcctcg atcgcattgc
     1801 tcgatggtct tatggtcgag gcgtacggtt ctgctgctcc tctcaatcag gtcgctacca
     1861 tggcggtgcc tgagttgagg atgatcaccg tgcaaccgtg ggatccgtcg ctgctgaccg
     1921 ctatcgagcg ggcgattctc aaatcggacc tgggggttac tccgggcaat gatggcaaga
     1981 ttatcaggat cgcaatccca tccctgaccg aggagcgtcg caaggatctg gtcaaggtgg
     2041 tccgaaaaat cgccgaggag ggtcgggtag cggtccgcaa cgctcgtcgt gaagccaacg
     2101 aatcgttgaa acgacaagag cgggagaagg agacctcagc ggatgagatc aagcacggtg
     2161 cggacatgat ccaggatctg accaatcggt tgatccatga gattgacggg ttgatggcca
     2221 agaaggaaaa ggagatcatg gagttttagg gactatcagt tgatagctga caactgatcg
     2281 ctgcgttata tgagcctgtc ctgcaaaccc ccgttcaccc ttcgacatgc tcagggcgaa
     2341 cggtatgggc attgaaaaga ttgacatttt tccgttcgtg ctgagcccgt cgaagcacaa
     2401 caaggggttt gcaggacagg ctcatatgca tctgaaaagg atcctcagtg ctgtcgtcct
     2461 cctcccagcc tttctcctgc tggttcagtt cggaaccccc tttcacttct tcttgcttgt
     2521 gacacttgcg atcctggttg gactgcatga gttctacggg atggcaaagg ccggtggctg
     2581 gcacccgttg acgcccctgg ggatggggag cggtctggcg ctaagttgca tggagttcct
     2641 cggagcgccg gcgccctggc tgatatcggc tgtggcgggg gtggtcgtcc tcctgcttat
     2701 cagcctcctg gtgggaggga cggagccgaa agaggctgcc tcgcgagggg caatcaccct
     2761 gctcggcctc atctatgtcg tggggttgct gagctttcca gcgctcctgc gggccatggt
     2821 gttggggcgc atctatatct tttaccttgt cttcgtcacc tgggcaggag acaccggggc
     2881 gttctatgtg ggctcgacga tggggaagcg gttgctctgt ccctccatca gcccgcgcaa
     2941 gaccgtagaa gggagcgtgg gcgggctgat gtgttctgtg ttagcctccg gcctggccaa
     3001 gttctggttc tgggaggagc tcgggactgt tgagtgcctc gtgatgggca tcgggcttgg
     3061 ggtgatgggc cagattgggg atctgtgcga atcgatgctg aaacgaggct ttggcgtgaa
     3121 ggacaccggc gcgctgatcc cgggccacgg cggtgtgctg gatcgagtgg atagcctcct
     3181 cttcaccggg ccggtcctct atgtggctgc cctggcaggg tgggtatgac gcc
//
LOCUS       DATMXI010000378         5832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_178642, whole genome shotgun sequence.
ACCESSION   DATMXI010000378 DATMXI010000000
VERSION     DATMXI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178642"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VN444_07290"
     CDS             <1..473
                     /locus_tag="VN444_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWR21647.1"
                     /translation="REQVLRIAEAGDCFNEVPIFDGGPNPANAQAVEPAALWGIRREG
                     MRRLVEAHPAIAIGFLKAFAGKLRYFTRKVEDLSFRSVTSRVAKHLLEMAEDDGKGGL
                     RLKQQSTQQEMASVVGTAREMIGRAFKALEKEGAITFDRHGVVIVSRAALIRML"
     gene            646..1212
                     /locus_tag="VN444_07295"
     CDS             646..1212
                     /locus_tag="VN444_07295"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HWR21648.1"
                     /translation="MMNESSCHGEGASTPEALRNVPDDRRVVLDVRDQVRAGQEPFQR
                     IMQSVMSLKDDQVFVLYNIFEPIPLYGVMAQRGFTHWTQKRGPEDWCITFYRADTQAA
                     AAPAPAAESRPAAPAGDAIVVDARGLEPPQPMAKILENLPRIAAGGHILAMTDRRPML
                     LYPKLEERGFSFSTEETTHGWFETRIWK"
     gene            1181..2509
                     /locus_tag="VN444_07300"
     CDS             1181..2509
                     /locus_tag="VN444_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21649.1"
                     /translation="MVGSKPASGSSAAPARQPSLWVPLRYFVTAQAAFVGALLWAPWQ
                     VDNLLDFYYQGNGLALTHLLTLGWITMTVMGASFQLVPVALETTLWSERLAGWQYWIM
                     LLGVALMVSHFWIGHHHGVAIGAGLVLIAITLFLINMGRTLWQLPRWDIIGRHVAAAL
                     VYLASTVVMGNLMALDKMFDFLGGQILRTIHAHAHLAGIGWVTMMIFGASYKLIPMFS
                     LSELRNERPAYWEFWLLQAGLAGLYVTLLLHSSWAALFALLIAAAVGLFLWNMREVLR
                     TRRRPRLDWGLQHSVSAMITLIFLTILGLWLSTGWISSDEFAARLAFGYGVLALLGWI
                     SVTIIGMMYKIIPFLVWHHRYSDLVGLRPVPAATQLLGESIPRIEYWLLHAGIAMTVA
                     GVIFASGLLLQVGTLVLALAGLTFAVAVCRIYRHLVPRLTPLPEAQTAGA"
     gene            2566..2910
                     /locus_tag="VN444_07305"
     CDS             2566..2910
                     /locus_tag="VN444_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWR21650.1"
                     /translation="MMTQPETSSITEEQIYSALRKLIDPELGVNIVDLGLVYDVQIEG
                     GQIGIRMTLTTRGCPMHASFVQAVDRAVREIHGVTGVTVEIVWEPAWNPDMISPEGKQ
                     ALSGMGRGGPAW"
     gene            2931..3980
                     /locus_tag="VN444_07310"
     CDS             2931..3980
                     /locus_tag="VN444_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWR21651.1"
                     /translation="MVISALRQVKYPGMSRDLVSFGMIKNARVEGATVYLDLQVPTED
                     AEVIAKVEASVREALSHIPGIGEIRIQAAPRPSPQESAPGPAPLPGVRRIVAVASGKG
                     GVGKSTVSVNLALALAQSGAAVGLLDADIYGPNVPRMLGELGRPKAHEGKIVPLVRHG
                     LKVISVGYLLGEQSPIIWRGPLVAQALKQLLHEVHWGELDYLIVDLPPGTGDTQLTLV
                     QAVPLTGGVIVTTPSAVALMDAERGLRMFREARVPILGIVENMSYFICPHCQGETDIF
                     SRGGGRQISDSLGVPFLGEIPLNPTIREGGDTGAPVVVAMPESSEAQLFRDVADKVRL
                     ATEAAAEAMPQMIIR"
     gene            4020..4925
                     /locus_tag="VN444_07315"
     CDS             4020..4925
                     /locus_tag="VN444_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HWR21652.1"
                     /translation="MSANYATTVDARVLPVPQKHPTIFRAFDGLAVGDTMLLVNDHDP
                     KPLYYTFAAERAGEFEWRYLEAGPEVWQVSIRRVAAAAGTHAPSEGMGCGGHHEHHEH
                     HDHHEHHGHEHAHTGSPTQILKDEHTLILQALDGLERKLIALEAGAAPDRAYFEKAVK
                     FIRTFADQCHHGKEEDLLFKTMVNRGFPLQGGPIAVMLSEHEAGRTYVRDMADAAAAI
                     GTDPTATQKIVRNGRAYIQMLRPHIDKENMILYAMADNLLSADDQARLGEAFERFEAE
                     KIGADVHNAMLALLDELKAGGHSCM"
     gene            complement(5074..5337)
                     /locus_tag="VN444_07320"
     CDS             complement(5074..5337)
                     /locus_tag="VN444_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR21653.1"
                     /translation="MTVQQKKGHHDPRTVGFSALYEAAKILSDQIDLEQVLGELEREL
                     VMRALKENGGVQARAAARLGITPRQLAYKMQKHRIIKEFRIEG"
     gene            complement(5429..>5832)
                     /locus_tag="VN444_07325"
     CDS             complement(5429..>5832)
                     /locus_tag="VN444_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HWR21654.1"
                     /translation="PASGFVGPMAAIAIGAVGGILCYSACMLKARLGYDDSLDVVGVH
                     GVGGTWGALATGLFASKAVNPDAANGLFFGNPHQFVVQVIAVLACMILAFVGTVILLK
                     LVDALVGLRVSSEEEQIGLDLTQHEENAYGF"
BASE COUNT         1078 a         1719 c         1861 g         1174 t
ORIGIN      
        1 ggcgtgagca ggtccttcgg attgccgaag ccggtgattg tttcaatgag gtcccgattt
       61 tcgatggggg accgaatccc gcgaacgccc aggcggtgga gccggcggcg ctctggggca
      121 tccggcgtga agggatgcgg cgtctggtgg aggcgcaccc ggccattgca atcggctttc
      181 tcaaggcatt tgccgggaag ctccgctact ttacccgcaa ggttgaggac ctgtcgtttc
      241 gtagcgtgac gagccgcgtg gccaagcacc ttctggagat ggccgaggat gacggcaaag
      301 gcggtctgcg cctgaagcaa cagtccacgc aacaggagat ggcgtccgtt gtgggaacgg
      361 cccgcgagat gatcggccgc gcgttcaagg ccctggaaaa ggagggcgcc atcacgtttg
      421 atcgccacgg ggtcgtgatt gtgagtcgag ccgccctgat ccgcatgctc taacctcgac
      481 cttgcccttc ctgtaggacg tgagactaaa gtcgcagaca cccctgaatc ttccccctat
      541 actacacttg aaccgatgag ttcaacgtgc aaggttcaaa gttcaacgaa acagaccgga
      601 cagacgagat agacgcagtt gacattttct aaggaggtaa tccctatgat gaacgagagc
      661 agttgtcacg gtgagggcgc gtcgacccct gaggcgcttc ggaacgtgcc cgatgaccgc
      721 cgggtggtcc tggatgttcg cgaccaggtg cgggcgggac aggagccgtt tcagcggatc
      781 atgcagtccg ttatgtcgct caaagacgat caggtatttg tcctctacaa tatctttgag
      841 ccgatcccgc tgtatggcgt gatggcgcag cgcggcttta cgcactggac acagaagcgc
      901 ggtcccgaag actggtgtat taccttctat cgggcggaca cacaggctgc ggccgctccg
      961 gcgccggccg ccgaatcgag accggcagcg cccgcagggg acgcgattgt cgttgatgcc
     1021 cgcggactgg agccgccaca gccgatggcg aagattctgg agaatctccc ccggatcgcg
     1081 gcaggcggac atatcctggc catgaccgac cggcggccga tgctgctgta tccgaaactg
     1141 gaggagcggg gcttcagctt ctccaccgaa gagacgacgc atggttggtt cgaaacccgc
     1201 atctggaagt agcgccgctc cggcccgcca gccgtccctc tgggtgccgc tgcgctattt
     1261 tgtgacggcg caggcagcct ttgtgggcgc cctgctgtgg gcgccgtggc aggtcgacaa
     1321 tctgctggac ttctactatc agggaaacgg cctcgccctc acccacctgc tgacacttgg
     1381 gtggatcacg atgacggtca tgggggcgtc attccaattg gtgccggtcg ccctggagac
     1441 cacgctgtgg agcgagcgcc tggccggctg gcagtactgg atcatgctgc tcggcgtcgc
     1501 gctgatggtg agccatttct ggatcgggca tcaccatggc gttgcgatcg gcgcaggtct
     1561 ggtgctgatt gccatcacgc tcttcctgat taatatgggg cgcacgctgt ggcagctccc
     1621 ccgctgggac attatcgggc gacacgtggc tgcggcgctg gtctatctgg cctccactgt
     1681 ggtcatgggg aatctcatgg cgctcgataa gatgttcgac ttcctgggag gtcagatcct
     1741 gcgcacgatc cacgcgcacg cccacctggc cggtatcggc tgggtcacca tgatgatctt
     1801 cggggcgagc tataagctga tccccatgtt cagcctgagc gagcttcgca atgagcggcc
     1861 ggcctactgg gagttctggc tgctccaggc cggtctcgcg ggtctgtacg tgacgctgct
     1921 gctgcacagt tcgtgggctg cgctgttcgc cctcctgatc gccgcagcgg tgggcctgtt
     1981 cctctggaat atgcgcgagg tcctgcggac gcgccgacgc ccgcgactgg actgggggct
     2041 gcagcattcc gtcagcgcca tgatcacgct gatattcctg acgatcctgg ggctgtggct
     2101 gagcaccggt tggatctcga gcgatgagtt tgccgcccgg ttggcctttg gctatggggt
     2161 cctggcgctt ctgggctgga tttccgtgac gattatcggt atgatgtaca agattatccc
     2221 attcctggtc tggcaccacc gttacagcga cctcgtaggg cttcggccgg ttccggccgc
     2281 cacccagctc cttggcgagt caatacctcg aatagagtac tggctgctcc atgcgggcat
     2341 cgcgatgacc gtggccggag tgatctttgc gtccgggctg ctcctccagg tggggaccct
     2401 cgtgttggcc ctggcgggtc ttacgttcgc cgtcgccgtc tgccggatct accgtcacct
     2461 ggttccccgt ctaacgccac ttcctgaggc gcagactgca ggagcctagg atccaccatc
     2521 gatcggcgga gcgagtcaag aagcacagga acaaagaaag gagatatgat gacgcaaccg
     2581 gaaacatcat caataactga agagcagatc tatagcgcgc tccggaagct cattgacccg
     2641 gagttgggag tgaatatcgt cgatctgggc ctcgtctacg acgtgcagat cgaaggcggc
     2701 cagatcggca tccgcatgac cctcaccacg cgtggctgcc ccatgcacgc cagcttcgtc
     2761 caggcggttg atcgcgccgt tcgagagata cacggcgtca ccggcgtcac cgtcgagatc
     2821 gtatgggaac ctgcctggaa tccggacatg atctcgcctg agggcaagca agccctgtcc
     2881 ggcatgggac gggggggacc cgcatggtaa cgccccagtt aacggaagat atggttatct
     2941 cggcgcttcg ccaggtcaag taccctggga tgagccgtga tctcgtctcg tttggtatga
     3001 taaagaatgc gcgggtagag ggcgcaaccg tctatcttga tctccaggtc ccgacggagg
     3061 acgctgaggt gatcgccaag gtggaggcgt cggtccgtga ggcgctgagc cacataccgg
     3121 ggatcggtga gatacgaatt caggccgccc cgcgcccctc gcctcaggag tccgctcccg
     3181 gaccggctcc gcttcccggg gtgcggcgca tcgttgccgt tgcgtccggt aaaggcggtg
     3241 tgggcaagag taccgtatcc gtcaatctgg ccctggccct ggcgcagtcc ggggctgcgg
     3301 tcggtcttct ggatgccgac atttatgggc cgaatgtgcc ccggatgctg ggcgagcttg
     3361 gccgtcccaa ggcgcatgaa ggcaagattg ttccgctggt gaggcacggc ctgaaggtga
     3421 tatcggtggg atatctgctg ggcgagcagt cgccgatcat ctggcggggt cccctggtag
     3481 cccaggccct gaagcaactc ctgcatgagg tgcactgggg cgaactggac tacctgatcg
     3541 tcgatcttcc accggggact ggggatacgc agttgaccct cgttcaggcg gtgccgttga
     3601 ccggcggggt gatcgtgacc accccttccg ctgtggcctt gatggacgca gagagaggcc
     3661 tgcggatgtt tcgtgaggcg cgcgtcccga tcctggggat cgtggaaaac atgagctatt
     3721 ttatctgtcc ccactgtcag ggagaaaccg acatcttcag ccggggcgga ggacggcaga
     3781 taagtgactc gcttggagtt cctttcctgg gggagatccc cctgaacccg actatccgcg
     3841 agggcgggga taccggcgct cctgttgtcg tggcgatgcc ggagtcctct gaggcgcagc
     3901 tcttccgcga tgtcgcggac aaggtacgcc tggcgactga ggctgccgcc gaggcgatgc
     3961 ctcagatgat catccgctaa caaccaacaa ccctgtatct ctctaaggag gaagtcagta
     4021 tgtcagcgaa ctatgcaaca accgtcgatg cccgtgtcct gccggttccg cagaagcacc
     4081 cgaccatctt ccgcgccttc gacggactgg cagttgggga cacgatgctt ctggtcaacg
     4141 atcacgaccc caagccgctc tactatacat tcgccgcaga acgagcgggg gagttcgaat
     4201 ggcgctatct ggaggccggt ccagaggtgt ggcaggtatc gattcgccgg gtagccgcgg
     4261 ccgccgggac gcatgcgcct agcgagggga tgggctgcgg cggtcaccac gaacatcacg
     4321 agcaccacga ccatcatgaa caccacggac acgagcatgc ccataccggt tcgccgaccc
     4381 agatcctcaa ggacgaacat accctgattc tccaggcgtt ggatgggctg gagcgaaagc
     4441 ttatcgcgct tgaggctggg gctgctccgg accgggccta tttcgagaaa gcagtcaagt
     4501 ttatccggac cttcgccgat cagtgtcatc acggcaagga ggaggatctg ttgttcaaga
     4561 cgatggtaaa tcgtggcttt ccattgcagg gcggaccgat agcggtcatg ctgtcggagc
     4621 atgaggccgg gcgcacctat gtccgtgaca tggccgatgc cgctgcggca atcggaaccg
     4681 atccgactgc gacgcagaag attgttcgga acgggcgcgc ctatattcag atgctgcgac
     4741 cccacatcga taaagagaat atgattctgt atgctatggc ggacaatttg ttgagtgcgg
     4801 acgaccaggc gcgcctggga gaggcgtttg agcgcttcga ggcggaaaag atcggcgcgg
     4861 acgttcacaa tgccatgctg gccttgctgg acgagttgaa ggccggcggt cattcatgca
     4921 tgtaggcgac tgggcgggcc aatagcgctg ctgttgagcc gtcaatctcg gccactcagc
     4981 gatatcgtta tctatctgaa tggtcaggag gcgtgagcct cctgaccatt tgcacgtcac
     5041 cgtcgttgcc ggactcgtcg aaggacgtga ggctcagcct tcgatgcgaa actctttgat
     5101 gatccggtgc ttctgcattt tgtaggcgag ttgtcgcggc gtgatgccca ggcgagcggc
     5161 ggccctcgcc tggacaccgc cgttttcctt cagcgcccgc atgactaact cgcgttcaag
     5221 ctcaccaagt acctgctcga ggtcgatctg gtcgctcagg atcttggccg cttcgtagag
     5281 tgcgctgaac cctacggttc ttgggtcgtg gtgccccttt ttttgttgca cggtcatgct
     5341 gaccatggct tttcaagatt agtgccaaca cgcggcgccc atcgattcac ctcggcacac
     5401 gccacgtgtt ggcaccatcc ctcctccctt agaagccgta ggcgttctct tcgtgctggg
     5461 tcaggtcgag accgatctgt tcttcttcac tgctcacccg caagccgacc agcgcgtcaa
     5521 cgagtttcag gaggatcacc gtacctacaa acgccaggat catgcaggcc agcacggcga
     5581 ttacctgaac cacgaactgg tgagggttgc cgaagaagag tccgttggcg gcgtcgggat
     5641 tgaccgcctt ggacgcgaac agaccggtgg ccagtgcgcc ccaggtaccg ccgacgccgt
     5701 ggacgcccac gacgtccagc gagtcgtcgt agccaagccg cgccttgagc atacaagcgg
     5761 agtagcacag gattcccccg acggcgccga tggcgatcgc cgccatcggg ccgacgaaac
     5821 cggacgccgg ag
//
LOCUS       DATMXI010000379         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_227119, whole genome shotgun sequence.
ACCESSION   DATMXI010000379 DATMXI010000000
VERSION     DATMXI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227119"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..539)
                     /locus_tag="VN444_07330"
     CDS             complement(147..539)
                     /locus_tag="VN444_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014026944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWR21655.1"
                     /translation="MKEFFVKTARKYQVIDITPRVQDIVEQDGSTIGLCCIFVPHATA
                     AVTINENADPNIGEDLHDALAKLIPEGVWRHDRIDGNAAAHIKAAILGPSEMVPVKDR
                     RLALGTWQSLMLVEFDGPRQRRVIVQIR"
     gene            complement(576..1436)
                     /locus_tag="VN444_07335"
     CDS             complement(576..1436)
                     /locus_tag="VN444_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWR21656.1"
                     /translation="MRLIDLHTHTTASDGVLSPQQLVRFAKDCNISVLAVTDHDTLEG
                     LPVAMEEAVQVGLQIVTGVEITAHAGDLEVHILGHFIDPDDNRLAEFLASSRNDRIER
                     AHRMIGKLWTLGLPLDADEVLRLARGSSVGRPHVAQAMINRGYVTSLKEAFDRYLSLG
                     KPGYVERARIPVALAIRAIKGAGGVPSLAHPGQYSHDEIIPSLVQQGLMGLEVYHLEH
                     DTESLFRYERACLKYDLLAVGGSDYHGTPGLRSSGLGRSAVPEARFEQLLAAASASHA
                     TGNSCKGGRG"
     gene            complement(1448..2257)
                     /gene="fdhD"
                     /locus_tag="VN444_07340"
     CDS             complement(1448..2257)
                     /gene="fdhD"
                     /locus_tag="VN444_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWR21657.1"
                     /translation="MSMHRIVRYRGGSCETVEIPVVAEHPLTIFVNGRELATLLCTPV
                     KLDCLVLGFLSFERIIQALDEVDSLEVFPEEAVADVRLTKAFTPPSHRILTSGCTGGI
                     TFGMPMEGFKTFPEEATLRPEQPFDLMKQLYAEAYLYRESRGIHAAALSDGKSLLLVA
                     EDVGRHNALDKIHGEALQQGMTTAGKILLSTGRISSEMLRKGAHMRTPIVISRTSPTS
                     LAIAAAKRFGITVIGYCRGEGFNVYSHPERLLPQRVATSSLSHIQQLTANS"
     gene            complement(2259..>2457)
                     /locus_tag="VN444_07345"
     CDS             complement(2259..>2457)
                     /locus_tag="VN444_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWR21658.1"
                     /translation="FFLELRWAFTASVAMAAAALFATGAGKTLLTKKAWWSSGLEMVG
                     IGLLVTVAGYGIGHFLSALWD"
BASE COUNT          499 a          761 c          712 g          485 t
ORIGIN      
        1 accttagcag gttggagcac acatgcctcg tttgagcccg tataaatgat tggtcaggtc
       61 tccgtttggg aaggcggcac acaggcatac tcgccaattc ttctaccata gtcttgaccc
      121 gattgtcaac ctgagaagga cgcagttcac ctgatctgga cgatcacccg ccgctgacgt
      181 ggaccgtcaa actccacgag catcaggctc tgccaggttc ccagcgccag cctcctgtcc
      241 ttcaccggca ccatttcact cgggccgaga atggccgcct tgatgtgggc cgcggcattg
      301 ccatcgatcc gatcgtgacg ccagaccccc tcggggatca gcttcgccag cgcatcatgg
      361 agatcctcgc cgatgttggg gtcggcattc tcgttgattg tgacagccgc ggtcgcatga
      421 ggcacaaaga tgcagcacag tccgatcgtc gagccgtcct gctctacgat gtcctggacc
      481 ctcggcgtga tgtcaatcac ctgatatttt cgagccgtct tgacgaagaa ctccttcatc
      541 tgttgacctg tcccgcctgg tctcgaggaa taccgctacc ctctgccacc tttacagcta
      601 ttcccggtgg catgggaggc agatgcggcg gcgagcaact gttcgaacct tgcctctggc
      661 accgctgacc tgccaagccc gcttgaccgg agcccaggag tgccatggta gtcagagcca
      721 ccgactgcca atagatcgta tttcaggcat gctcgctcgt aacggaatag cgattcggta
      781 tcgtgctcca ggtgatacac ctccagtccc ataagtccct gctggacgag ggatggaatg
      841 atctcgtcgt gactgtactg ccctgggtgt gcgagagatg gcacacctcc agcccccttg
      901 atggcacgta tcgccagggc gacaggaatt ctggcgcgtt ccacgtagcc tggcttaccc
      961 agagacaggt accggtcaaa ggcctccttc agcgacgtca catagccgcg attgatcatc
     1021 gcctgcgcca catgggggcg cccaactgag gagcctcgcg ccaagcgtaa gacctcatca
     1081 gcgtccagcg ggagaccaag agtccagagc ttcccgatca tccgatgagc cctctcgatt
     1141 cggtcgttgc gggaggaggc gaggaattcg gcgagccggt tatcgtccgg atcgatgaaa
     1201 tgtcccagga tgtgaacctc caggtccccc gcatgcgcgg tgatctcgac gcctgtaacg
     1261 atctgcaggc ccacctgcac ggcctcctcc atcgccacgg ggaggccctc cagggtgtca
     1321 tgatcggtga cggccaggac tgagatattg cagtccttgg caaagcgaac caattgttgc
     1381 ggagagagga cgccgtcaga agcagtcgta tgggtatgca gatcgataag gcgcatcacg
     1441 tgagctgtca gctattcgct gtcagctgtt ggatgtggga caggctgctg gtcgccaccc
     1501 gctgtggaag cagacgctcg gggtgactat agacattaaa cccttcgccc cggcagtagc
     1561 cgatcacggt aatgccgaag cgcttggccg cggcaatagc gaggctggtg ggcgaggttc
     1621 gagagatgac aattggggtc cgcatgtggg cccctttacg aagcatctca gacgagatgc
     1681 gaccggtgga gaggaggatc ttgccggcag tcgtcatccc ctgctgaagc gcttcgccgt
     1741 ggatcttgtc cagggcgtta tgccggccga cgtcctcggc gacaagtagc aggctcttgc
     1801 cgtcgctcag ggcggcggcg tgaatgccac gcgactcccg gtagagatac gcctcggcat
     1861 acaactgttt catgagatcg aacggttgct ccgggcgcag ggtcgcctcc tccgggaatg
     1921 ttttaaaccc ctccatcggc ataccgaatg tgatgcctcc ggtgcaccca gaggtcaaga
     1981 tgcggtgact cggtggggtg aacgccttgg tcagtctgac gtctgccacc gcctcctccg
     2041 gaaagacctc cagcgagtcg acctcgtcga gcgcctggat gatcctctca aaactcaaaa
     2101 aacccaggac caggcaatcg agcttcaccg gcgtgcacag caacgtagcc agctcccgcc
     2161 cgttgacaaa gatcgtgagg ggatgctccg ccaccactgg aatctctacc gtctcgcacg
     2221 atccaccgcg ataccgaacg atccgatgca tggacatctt agtcccagag ggcactgagg
     2281 aagtgcccga tgccataccc tgcaaccgtg actaagagcc cgatccccac catctcgagc
     2341 ccgctgctcc accaggcttt ctttgtcagg agcgttttac ccgccccggt tgcaaagagg
     2401 gccgccgcgg ccatggcgac cgaagccgtg aacgcccatc tgagctcgag gaagaag
//
LOCUS       DATMXI010000380         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_271739, whole genome shotgun sequence.
ACCESSION   DATMXI010000380 DATMXI010000000
VERSION     DATMXI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271739"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..491
                     /locus_tag="VN444_07350"
     CDS             129..491
                     /locus_tag="VN444_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21659.1"
                     /translation="MATILIIDDEESIRNLLKEVLERANHRVLEARDGREGLALYQQN
                     KVDLVLMDILMPGTDGLEATLQLTKEYLDAKVIAMTGAQAERNFLDVAKLFGACRVFE
                     KPFNLDQLMQAVNEELAK"
     gene            complement(548..1402)
                     /locus_tag="VN444_07355"
     CDS             complement(548..1402)
                     /locus_tag="VN444_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21660.1"
                     /translation="MFKIKKKAEKPRPVILYNIIEDYSEFDAPGNVTACAMTYPEDLP
                     AHAKVLSESYDIPLETMTTLLTDGGVYVYPQVGGLLTRGLFACRIDPTGEVPKQTWIL
                     DLTQFAEAEQLAQARGMKLDEVAALVFHWTLPEELRTKLEQKNLGIDYRTLDRAVAKG
                     GDIKYIDFRKDWSPHFKRLCIMPDGRLIETGGLEEFAQLHSITSAQAKTLVEQGGTLE
                     VNGELLACQIVNGQPAVARFSAQNYKQAKELAATKRLHLMDALSEVAYNDPVMMRSLA
                     RKELSAGR"
     gene            complement(1449..>2147)
                     /locus_tag="VN444_07360"
     CDS             complement(1449..>2147)
                     /locus_tag="VN444_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWR21661.1"
                     /translation="GLNAGQIIRQRRSAVSFDGKTSITTTTFFRMMQRVVSRPDLPQL
                     DRPMPWDVWPYDPAIHLMLFVHRVEGLTPGLYFLARDPQKLSPIQQCMNPELTWAPAP
                     GCPENLPLYWLFEGDARKLATQVSCHQDIAGDSAFSLGMLAEFEGRLRERGAWWYPRL
                     FWEAGLLGQVLYLEAEAAGVRATGIGCFFDDPVHEIMGIKERSFQSLYHFTVGGPVED
                     SRLMTLGAYHHLNR"
BASE COUNT          492 a          628 c          552 g          475 t
ORIGIN      
        1 ggagcgcgca ttatatacaa gttgtagctc aggctccaag gggtgaggac agtctcctcg
       61 atcaacttct gctagacaga ggaacccaac ataggtaaga tgaaagaatt ggaggggtat
      121 ttcacgcgat ggccacgatc ctcattatcg acgacgaaga atcgatccgg aacctcctga
      181 aggaagttct tgagcgggcc aaccaccgtg tgctcgaagc ccgcgatggg cgggaaggcc
      241 tagcgctcta ccagcagaac aaagtcgatc tggtcctcat ggatatcctg atgccgggaa
      301 ccgatggcct cgaagccacc ttacagctga cgaaagaata tctcgacgcg aaggtcatcg
      361 ccatgaccgg tgcccaagcc gaacgaaact ttcttgatgt ggcgaaattg tttggcgcct
      421 gtcgtgtctt cgaaaaacca ttcaacctcg atcaactcat gcaagccgtc aacgaagagc
      481 tcgccaagta gcgctcctct gtgctctgac aaccatcagc gtgggccggc ctgtttacag
      541 tattgactca tctgcccgca ctaagttctt tcctagccaa cgagcgcatc atcaccggat
      601 cgttgtaagc cacctcgctc aacgcatcca tcagatgtaa tcgcttcgtc gccgccaact
      661 ccttcgcctg tttgtagttc tgcgcactga atcgagccac tgccggctgg ccattcacaa
      721 tctgacaggc caggagctcg ccgttcactt ccagtgtgcc accctgctca accaacgttt
      781 tggcttgcgc cgatgtgatg ctatgaagtt gggcaaactc ttccaagcct cctgtctcaa
      841 ttaatcggcc atccggcatg atgcagagtc gcttgaaatg gggggaccag tccttccgga
      901 aatcgatgta cttgatatcg ccacccttgg caacagcacg gtccaacgtg cggtaatcga
      961 tcccaagatt cttctgctcg agttttgttc tcaactcctc cggcagtgtc cagtgaaaaa
     1021 cgagcgccgc gacttcatca agcttcatcc cccgcgcctg agccaactgc tccgcctcgg
     1081 caaactgggt gagatccaag atccaggtct gtttcgggac ttcaccggtt ggatcgattc
     1141 gacaggcgaa tagccccctc gtcagcaacc cgccgacttg ggggtacaca tagaccccgc
     1201 catcagtcag gagggtcgtc atcgtctcca gcggaatatc gtagctttct gacagaacct
     1261 tggcatgtgc gggcaggtcc tctggatatg tcatggcgca ggccgtgaca ttccctggtg
     1321 cgtcgaactc cgagtagtcc tcaatgatgt tatagaggat aacaggtctt ggcttctcgg
     1381 cctttttttt aattttgaac atgagacgta gcgctccagg ctaatggtgt agatataatc
     1441 ttacattctc atcgattcag gtgatgatag gcccccagcg tcatcagtct gctatcctcg
     1501 accggaccgc ctaccgtaaa gtggtagagc gactgaaacg aacgttcttt gatccccatg
     1561 atctcatgga ctgggtcgtc aaaaaaacag ccgattccag tggctcgcac cccggcagct
     1621 tccgcctcca ggtagagcac ttgtcccagc agaccagcct cccagaagag acgtgggtac
     1681 caccaggctc ccctctcccg caagcgccct tcgaactcag ccagcatccc caatgagaag
     1741 gcactgtccc cggcaatgtc ctgatgacag ctcacctggg tggcgagttt cctagcgtcg
     1801 ccttcaaaca gccaatagag cggtagattc tccggacaac ccggggccgg agcccaggtc
     1861 agctccggat tcatgcattg ttggatgggt gatagcttct gtgggtcacg ggccagaaaa
     1921 tagagccctg gcgtgagacc ttcgacgcga tggacaaaga gcatcaagtg aattgccgga
     1981 tcataaggcc agacgtccca gggcatcggt cgatccaatt gcggcaagtc cggacgagac
     2041 accacgcgtt gcatcatgcg aaaaaatgtc gtcgttgtga tcgatgtctt gccatcgaac
     2101 gataccgcac tccggcgttg tctgatgatc tggcccgcat tgaggcc
//
LOCUS       DATMXI010000381         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_123316, whole genome shotgun sequence.
ACCESSION   DATMXI010000381 DATMXI010000000
VERSION     DATMXI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123316"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="VN444_07365"
     CDS             complement(<1..2035)
                     /locus_tag="VN444_07365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HWR21662.1"
                     /translation="MRSFRQAIVDEILPEVSKPSRYIGMEWNAVKKNPAATSVKIALA
                     FPDTYEIGMSHVGLKILYQIVNRRPEFMAERVYAPWPDAEALLRRRRIPLCTLESGYS
                     LADFDLIGFTLQYELSYTTILNMLDLAGVPLGSAERREGDPFVIAGGSVGFNPEPIAE
                     FIDLFVLGDGEEVVLDVCEATAAWKASGGRRDALLEAWARIPGCYVPALHQGETIRKR
                     TVIHLDGIDYGAFPVPFMEIVHDRVSIEVMRGCTQGCRFCQAGYLYRPLRERSSEEIQ
                     QLAMHAVRQTGYEEVSLASLSIADLTVLPDVVPSLMDRLLPEKISLSLPSLRVETLNR
                     FPQVAEEISRVRKTGFTIAPEAGSARLRKVINKEGFDEEQIFTAVRNAARAGWESVKF
                     YFIIGLPTETQEDLDEIVRITRESARIARAESARGFGLTVSASSFVPKPHTPFQWCAQ
                     DPMEMLKDKQEFLRRKLREIRVSYKWHNVQSSFLEAVFSLGDRSLGRVVRRGYELGCR
                     FDGWTEHLKFDRWMQAFDETGIDPRAFANRPRNVDEPLPWDHIDSGVNKAFLQREYKK
                     AMEVRGTPDCHTAACTACGEICMPSWPTWAEEVGMKVSSLEFGVSSSHCRAPSTEPET
                     RNPKPETSSEAPVQRIRCVFQKIGMLRFLSHLELMRALGRALRRAGVALAYSQG"
     gene            complement(2179..2955)
                     /locus_tag="VN444_07370"
     CDS             complement(2179..2955)
                     /locus_tag="VN444_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWR21663.1"
                     /translation="MARDAREKTGSRQPMARQTLLMEALIGLIRAAARQLDPVILQET
                     VRGVGAQLGRQAVSEYCHIRQAGGRPKGYTWAECLKEIGQQFGWTLRVAVESEGVIRI
                     DVLGCQVAESDESGSHLCDLGSALFGGAMAEAIGDVKVCASRCSETPPLHCTFAIYYR
                     SSEESLAMPGVVYPRMSDPVTPLVEEPLRRSLGARLTSRETQVLQHIAQGLPDREIAE
                     SLQLSVRTVENHAARIRKKLQVDTRTALVRYALRARLIEP"
BASE COUNT          669 a         1111 c          930 g          630 t
ORIGIN      
        1 acccctgcga gtaggccaga gcaaccccgg cccgacgcag cgctcggccc agcgccctca
       61 tcaactccag atgcgacaga aaccggagca tcccgatctt ctgaaaaaca caccgaatgc
      121 gctgcacagg cgcttcgctt gatgtttcgg gtttcgggtt tcgggtttcg ggttcagtgc
      181 ttggcgcccg acaatgtgaa ctcgaaactc caaactccaa actcgaaact ttcattccga
      241 cctcctccgc ccaggtcggc caactcggca tacagatctc gccgcaggcc gtacaggcgg
      301 ccgtatgaca atcgggcgtc ccgcgaacct ccatggcctt cttgtactcc cgctgcagga
      361 acgccttgtt gacaccggag tcgatatgat cccacggcaa cggctcatcc acgttacgcg
      421 gccgattcgc gaacgccctc ggatcgatcc ctgtctcgtc gaaggcctgc atccaccggt
      481 cgaacttcag atgttcggtc cagccgtcaa accggcaccc cagctcgtag ccgcgccgga
      541 cgacgcgccc aagcgaacgg tcacccagcg agaacaccgc ctccagaaac gaggactgga
      601 cgttatgcca cttatacgac accctgatct cccgcaactt ccgtctcagg aactcctgct
      661 tgtccttgag catctccatc gggtcctggg cacaccactg gaagggcgta tgcggcttcg
      721 gcacgaacga ggaggcgctg accgtcagtc caaagcctct cgccgactcg gcccgcgcga
      781 tccgcgccga ttcccgcgtg atccgaacga tttcgtccag gtcctcctgt gtctcggtcg
      841 gcagtccgat gataaagtaa aatttgaccg actcccaacc cgccctggcg gcgttccgca
      901 cggccgtaaa gatctgctcc tcgtcgaacc cctccttatt gatcaccttc cgaagtcgcg
      961 cgcttccggc ctccggcgcg atggtgaagc cggtcttccg aacccggctg atctcctcgg
     1021 ccacctgcgg aaaccggttc agcgtttcga cccgaaggga gggcagcgac agtgagatct
     1081 tttccggcag gagccgatcc atcaacgaag gcacgacatc gggcaggacc gtcagatcgg
     1141 cgatactcaa cgaggcgagc gagacctctt cgtatcccgt ctgccggacc gcatgcatag
     1201 ccaattgctg aatctcctca ctcgatcgtt cccggagcgg ccggtacagg tagcctgcct
     1261 gacagaaccg gcacccctgg gtgcagcccc gcatcacctc aatgctcaca cggtcgtgga
     1321 cgatctccat gaacgggacc ggaaaggcgc cgtaatcgat cccgtccaga tggatcaccg
     1381 tccgcttgcg gatcgtctcg ccctggtgga gtgccgggac gtagcagccc gggatcctgg
     1441 cccaggcttc aagcagcgca tcacgccgcc cgcctgaggc tttccatgcg gctgtcgcct
     1501 cgcagacatc gaggacgacc tcctcaccgt cgcccaggac gaacaggtcg atgaactccg
     1561 cgatcggctc ggggttgaag ccgacggacc cgcccgcgat cacaaatgga tcgccttccc
     1621 gtcgctcagc gctccccaaa gggacgccgg ccaggtccag catgttcaga attgtggtgt
     1681 aggacagttc gtactgcagc gtgaagccga tcaggtcgaa atcggccagg ctgtaaccgg
     1741 actcaagggt acagagcgga attcgcctcc gccgcagcag cgcctccgca tcaggccagg
     1801 gcgcatagac ccgttccgcc ataaactccg gccgtcggtt cacgatctgg tacagaatct
     1861 tcaggccgac atgggacatg ccgatttcgt aggtatcggg gaaggcgagg gcaatcttga
     1921 cggatgtcgc ggcggggttc ttcttaacgg cgttccactc cattccgata tagcgagacg
     1981 gcttgctgac ctctggaaga atctcgtcca ctatcgcttg tcggaagcta cgcactcatg
     2041 cctctcctta tttacgcgtg gttacgccgt attgcagaat atcatacacg aaggccgtcc
     2101 gaaaggcaat cttggagcag tgcgaatgag gcgcgacggg gcgagaagcg gcacgttgag
     2161 gataggacct ctgcgactct atggttcaat caagcgagca cgcaacgcat accgcactaa
     2221 cgctgttcgg gtatcaacct gaagcttctt gcggattcgc gcggcatggt tctcgacggt
     2281 ccgaacggac agttggaggc tctcagcgat ctctctgtcg ggtaaaccct gcgcgatatg
     2341 ctggagtacc tgcgtctcac gcgacgtcag gcgcgcgccc aggctgcggc gtaacggctc
     2401 ctcgacaagt ggagtcacag gatcgctcat tcgcggatag acgacccctg gcatcgccag
     2461 gctctcctcg ctgcttcgat agtagattgc gaaggtacaa tggagcggtg gcgtctccga
     2521 gcatctgctc gcacagactt tgacgtcacc gatcgcctcc gccatggccc cgccaaagag
     2581 cgcggagcct aaatcgcata ggtgcgatcc ggactcatcc gactccgcga cctggcaccc
     2641 cagcacgtca atgcgaatca cgccctcgga ctcgaccgcg acccgaagcg tccagccgaa
     2701 ctgctgccct atctccttca gacactcggc ccaggtgtag cctttcggtc gtccacccgc
     2761 ctgacgaatg tgacaatact cggacaccgc ctgccgaccc aactgcgccc cgacaccgcg
     2821 aaccgtctcc tggagtatca cagggtcgag ctgccttgcg gcggcccgga tcagtccaat
     2881 cagcgcctcc atcagcagtg tctgccgagc catcggttgc cgactaccgg tcttctcgcg
     2941 agcgtcacgg gccatcccag gctttcctca tacccacctt gtgtccggta caggaacaag
     3001 ggaccgtaca atacgaccgt atgtagatga cagcgaagcg ttaacccgag tataatatag
     3061 ggtgataaga gcaaatgtac cacctctctc tctggttttt tgcgcgcggg tatgccctac
     3121 tccctatcca ttgcccacag gtgcgcgaag cgcaaaccgg accagcgccg tgcggctgcc
     3181 gatcccaagc ttatggcgaa tctgcgcggc atgactcatt ccctcgagtc tgcccgcggg
     3241 cgtctgcgac tcagcgcttc agcaactccg cgacagtctg taacagggta cgggattcga
     3301 ggggtttggt gaggtaggcg gcgcaaccgg cttgcagggc
//
LOCUS       DATMXI010000382         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_139291, whole genome shotgun sequence.
ACCESSION   DATMXI010000382 DATMXI010000000
VERSION     DATMXI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139291"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /gene="murD"
                     /locus_tag="VN444_07375"
     CDS             complement(<1..1121)
                     /gene="murD"
                     /locus_tag="VN444_07375"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWR21664.1"
                     /translation="MDANLAGKRVVVVGLARSGEAACRLLLKQGATVIGTDRRGEREI
                     GADLCSLEQDGVSLELGEHHLHSLLSADLVVVSPGIDLREPLFQRVREVGIPLIGEVE
                     LAYRCSEATFIGITGTNGKSTTTTLLGAMLKQAGLPSHVAGNIGTPLCRVAPSLAAGE
                     CVVTELSSFQLETIEEFRPHVALLLNLAPDHLDRYDQVEDYYRAKARIFENQRPSDFT
                     VINADDPLVLQVSAQAQGRRVAFSRTRPLDMGAYVEGDQLMLALNGMREVMCRISELK
                     IQGVHNLENALAAGLAAAVAGAPTTAIRIALTSFEGLEHRLEFVAEINGVRYINDSKG
                     TNVGAVVRSLESFTNPVVLIAGGRDKHGDFAPLIPLVRER"
     gene            complement(1231..2313)
                     /gene="mraY"
                     /locus_tag="VN444_07380"
     CDS             complement(1231..2313)
                     /gene="mraY"
                     /locus_tag="VN444_07380"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWR21665.1"
                     /translation="MLYHLLFPLHGSYAVLNVFRYVTFRTAGAILTALLISLLMGPAL
                     IRKLQELQIGQSIRDDGPAGHLQKAGTPTMGGLLILVSVLIATLLWANLTNRFVWIAL
                     FCTVWMGAVGFIDDYLKVVSRNSKGLSVRTKLLWQVIPSMLIGLFLYINPVDPFTTKL
                     TIPFLKDWMPDLGWGYVLFVTLVIVGTSNAVNLTDGLDGLAIGPILMTAAAYTVLAYI
                     AGHASIAHYLQVVFVRGSSELTVFGGAIIGASLGFLWYNAYPAQLFMGDTGSLALGAA
                     LATLAVLVKNELLLLIVGGVFVAEAISVIMQVFSYRTTGRRVFRMAPLHHHYELNGMA
                     EPKIIVRFWIISFILALLSLTTLKLR"
     gene            complement(2414..3724)
                     /locus_tag="VN444_07385"
     CDS             complement(2414..3724)
                     /locus_tag="VN444_07385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWR21666.1"
                     /translation="MSGAIRRIGLIFGSRSVEREISIMTASKIYEVLLSLQDQFETLP
                     IFLTAEGVWLTGEAVRDLLTVDAEIRKLAERSVVAGTAERSKLDAEKLRLNRERYIPQ
                     LDNLDRGQSATGVDPLFLAPDPSVGALVPQQERKGWLRKQLHPTIDVAFPVIHGTHGE
                     DGTIQGLCELADLPYVGAGVTASAVGMDKIIAKLIFQGAGLPVVEGIGIARRELLEDE
                     AAVVKGIERRLNYPVIVKPAVAGSSVGIGMAHDAAEALSLAKRAMRFSSRVLIERAVE
                     QRVEVQCGVLGNHALTVSKCEEIISSGEVVGYRDKYPEDTQPGSADLAPSVIPARIPQ
                     ALSDEIRSMAAAAFQAIDSRGISRVDFLIDSAAMKPYVNEVNTMPGSLSFTLWEASGV
                     KPAELVVRLVDLALEAHREKRSTHFKSTEGRALVDRRHLVTPGK"
     gene            complement(3721..>4680)
                     /gene="murF"
                     /locus_tag="VN444_07390"
     CDS             complement(3721..>4680)
                     /gene="murF"
                     /locus_tag="VN444_07390"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWR21667.1"
                     /translation="VNTTMGIVRVVREGLRDDHRFFVVEVAAYGPGEIREVCEVLRPR
                     IGILTAVGVQHLERFGTPERIAEAKYELIDSLPPDGLAIVNADDPGALRLAERARREG
                     RRVLLYGVGEDERALGVRGKDIKLSSHGSAFRVETGYGTAMFETKLLGGWNIANILGA
                     TAAALECGVSLEEIAVGVKSLTPAPKRLELREEGGVIKLIDVANANPLGAQMALEVLS
                     QFKGGSKILITPGLVELGQIEAEENRRFGRAAAAVCDYVLLVGPQQTQPLREGLLEGG
                     FADSRVLVARHSGEVTDHLKAIVREGDVLLYENRLPDTYLELA"
BASE COUNT          996 a         1541 c         1259 g          884 t
ORIGIN      
        1 cgctcgcgga ccagggggat gagcggcgcg aaatcgccat gcttgtccct accgccggcg
       61 atgagcacaa cggggttcgt aaaactctcc aacgatcgga caaccgcccc aacattggtc
      121 cctttggagt cgttgatgta gcgaacaccg ttgatctccg cgacaaactc cagacgatgt
      181 tccagcccct cgaaactggt gagcgctatc cggatcgccg ttgtgggggc gccggctacc
      241 gccgccgcca ggccggcggc cagcgcattt tccagattat gcacaccctg aatcttcaat
      301 tcggatatcc ggcacataac ctctcgcatc ccattaaggg ccagcatgag ctgatcgcct
      361 tcaacatacg cccccatatc gagcggcctg gtccggctga atgccaccct ccgtccttgg
      421 gcctgtgccg acacctggag gaccagcgga tcgtccgcat tgatgacggt aaagtcggac
      481 ggccgttgat tctcgaagat gcgagccttg gcccggtaat agtcttcgac ctgatcgtag
      541 cgatcaagat gatcaggggc gagattcagc agtaacgcca cgtgcggcct gaactcttcg
      601 atcgtttcca actgaaagct cgatagctcc gtgaccacac attcacccgc cgcaagggaa
      661 ggcgccactc ggcagagggg ggtaccgatg ttgccggcca catgcgaagg caggccggct
      721 tgcttcagca ttgcgccgag aagggttgtg gtggtgctct tcccgttggt tccagtgatc
      781 ccgatgaatg tggcttcact gcaccggtag gccagttcga cttcaccgat gagcgggatg
      841 cccacctcac gaacccgctg aaacagcggc tcacgcagat cgatcccagg acttaccacg
      901 acgagatcgg ccgagagcaa ggagtgcaga tgatgctcgc ccagctcaag gctgaccccg
      961 tcctgctcca ggctgcagag atcagcgcca atctcacgct cacccctgcg gtcggtgccg
     1021 atgaccgtgg cgccctgttt gagcaacagg cggcaggccg cctccccgga tcgcgccaac
     1081 ccgacgacca cgactcgttt gcctgccaga tttgcgtcca tagcgttcat agcgttcata
     1141 gcgtttgttg cgtctgttgc gttcattgcg tctgttgcgt tcattgctat agacgctgca
     1201 cgctgcacgc aatagacgct atagacgctt ttacctcaac ttcaacgtgg tgagcgagag
     1261 cagggccagg ataaaggaga tgatccagaa ccggacaatg atctttggct ccgccatccc
     1321 gttcagctcg tagtggtgat ggaggggcgc catcctgaag actcgtcggc cggtcgtccg
     1381 gtaggagaag acctgcatga tcaccgagat ggcctctgcc acgaacaccc ccccgacgat
     1441 gagcaggagc aactcgttct tgaccaggac agcgagcgta gccaaggcgg ccccaagcgc
     1501 taaagagccg gtgtcgccca taaagagctg cgcggggtac gcattgtacc agaggaatcc
     1561 caggctggcc ccgatgatgg caccgccaaa gacggtaagc tccgagctcc ctctcacaaa
     1621 gaccacctgc aggtagtgag cgatgctggc atgtcctgca atgtacgcca gaaccgtata
     1681 cgctgcggcg gtcatcagga tcggaccaat ggccagccca tccagaccat cggtcaagtt
     1741 taccgcattt gaggtgccaa cgatgaccag cgtcacgaac aggacgtagc cccagcccag
     1801 atcgggcatc cagtccttga gaaaggggat agtcagcttc gtagtaaatg gatcaaccgg
     1861 gttgatatac agaaagagcc caataagcat gctgggaatt acctgccaca acagttttgt
     1921 cctgaccgag agtcccttac tattcctgga caccaccttc aggtagtcgt cgatgaaccc
     1981 caccgccccc atccaaaccg tgcaaaacag ggcaatccag acgaatcgat tggtcaggtt
     2041 cgcccagagc aacgtggcga tgagcacaga gaccaggatc aggaggccac ccatcgtggg
     2101 cgtcccggcc ttctgcagat gcccagccgg cccgtcgtcc ctgatgctct gaccgatctg
     2161 cagctcctga agcttgcgga tcaacgcggg tcccatcagc aaactgatca gcagcgcggt
     2221 gaggatggcc ccggccgtac ggaaggtcac gtaccggaag acgttcaaaa cggcgtagct
     2281 tccgtgcaat gggaaaagca ggtgatacag cataaaacct cattagaggg tatagggtat
     2341 agggtttagg gtttaggagt tctccgtctc gatcctcccc tagaccctac accctttacc
     2401 ctataccctg ctcttatttg cctggcgtca ccagatgccg ccgatccacc agcgccctgc
     2461 cctctgtcga tttgaaatgg gttgaacgct tttctcgatg ggcctcaagc gccaagtcca
     2521 ccagacgaac gacaagctcc gcaggcttca ccccgcttgc ttcccagagg gtaaaggaaa
     2581 gcgaccccgg catggtgttg acctcattga cgtacggctt catcgcggct gagtcgataa
     2641 gaaaatcaac ccgggagatc ccgcgcgagt cgatcgcctg aaaggctgct gctgccatgg
     2701 accgtatctc atcagaaagc gcctggggaa tcctggcggg aatgacactc ggcgctagat
     2761 ccgcgcttcc cggctgcgtg tcctccgggt acttgtcacg gtagcccaca acctcgcccg
     2821 agctgatgat ctcctcacac ttcgagacgg tcaacgcgtg attgcccagg acgccgcatt
     2881 gcacctctac ccgctgctca acagcccgct ctatcagcac gcgggagctg aaacgcattg
     2941 cgcgcttcgc caggctcagc gcttcggccg catcatgagc catacctatg cccacgctgg
     3001 aaccggccac ggcaggcttg actatcaccg ggtaattcaa tctgcgctca atccccttca
     3061 caacggccgc ctcatcctcc aacagctcgc gtctggcgat accgatcccc tcaacaaccg
     3121 gaaggcctgc gccctgaaag atgagcttgg cgataatctt atccattcct acggccgagg
     3181 cggtgacccc agcccccaca tacggcagat cggccaattc acacagaccc tgaatcgttc
     3241 catcctcacc atgcgtccca tgaatgaccg ggaaggcgac atcgattgtg gggtggagct
     3301 gcttacgaag ccagcctttt cgctcctgct gcggcaccaa cgcgccgacc gacgggtccg
     3361 gcgccagaaa gagcggatca acccccgtcg cgctctggcc ccggtccagg ttgtcaagct
     3421 ggggtatgta tcgctcccga ttcaaccgca gcttttccgc gtcgagcttc gatcgctcag
     3481 ccgttccagc cacaacgctt cgttctgcca gctttcgtat ttcggcatcg accgtcaaca
     3541 gatcccgaac cgcctcaccg gtaagccata cgccctctgc cgtgagaaag atcggcagcg
     3601 tctcgaactg gtcctgcaac gagagcagga cctcatagat cttactggcg gtcatgatcg
     3661 aaatctctcg ctccaccgag cgacttccaa agatcagccc tatgcgtcta atggcgccac
     3721 tcatgcgagc tccagatacg tatcaggtag tcgattctcg tacagcagga catcgccctc
     3781 ccggacgatc gccttgaggt ggtcagtcac ttcaccgctg tgtcgagcca ccagaacccg
     3841 gctatcggca aacccaccct cgagaagccc ttcacgcagc ggttgtgtct gctgtggccc
     3901 aaccaacagg acgtaatcgc agaccgcagc ggcagctcgg ccaaaccgtc ggttctcctc
     3961 cgcctcgatc tggcctaact ccaccaggcc tggcgtgatc agaatcttcg agcctccctt
     4021 aaactggctg aggacctcca gggccatctg ggccccaagc ggattggcgt tggccacatc
     4081 gatcagcttg atgaccccac cctcttcgcg gagctccagg cgcttgggcg ccggcgtaag
     4141 agacttcacc ccgacggcaa tctcctccag ggacacaccg cactctaagg cagcagccgt
     4201 cgcccccagg atattggcaa tgttccagcc tcccagcagc ttggtctcga acatggccgt
     4261 gccatacccg gtctccacgc gaaaggccga gccgtgactg gagagcttga tatctttccc
     4321 ccggacgcca agagcccgct cgtcctcacc gaccccgtac aagagtacgc gccgaccttc
     4381 ccgcctcgcg cgttcggcca atcgcagagc gcctggatcg tcagcgttca cgatcgccaa
     4441 gccatccggt ggaagcgaat cgatcagctc atacttcgcc tcggcgatcc gctccggcgt
     4501 gccgaatcgc tcgaggtgct ggaccccaac cgccgtgaga ataccgattc gaggccgcag
     4561 gacctcgcac acctccctga tctcaccggg gccgtacgcc gccacctcaa cgacaaagaa
     4621 gcggtgatcg tcccgcagcc cctcgcgaac gacccgcacg atacccatcg tggtattgac
//
LOCUS       DATMXI010000383         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_10972, whole genome shotgun sequence.
ACCESSION   DATMXI010000383 DATMXI010000000
VERSION     DATMXI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10972"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VN444_07395"
     CDS             <1..352
                     /locus_tag="VN444_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21668.1"
                     /translation="VISNGYDYLLLDGILCLACVFPAFALICQYLSPVERDLAPYSSH
                     SDYKDIRTSSPSHWGSDPWKRALSAGEQDWIGLSILKRGLMTNALDEIDVEARETRIV
                     GGLLAVVKYEGICY"
     gene            complement(346..2166)
                     /locus_tag="VN444_07400"
     CDS             complement(346..2166)
                     /locus_tag="VN444_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWR21669.1"
                     /translation="MVTMPVIPSRTVNSRGLTSLCLLISLMMVACAAAPQMPEKLALA
                     DPPKAASSVHPPDAGASYHFMLGYQAELAQDMDRAIREYQAALKADPASQTVKARLAS
                     LYFSLGDMPNALLYADQVAEGGSQDGQLLTQMAGILASAGQGERAVKLLDRAIAADPT
                     SGDPYFTKGLLLLNLKRQPEAEQSIRTGLAKSPDSAVGHYHLGRLLLDAGKGEEAAAS
                     FERSIAANASFEPAYHALASVYESRQSQDRAIAVLRKYLQTVNPRNRAIRHQLVRVYM
                     AAKDYQGARKELNDLLAEDPSDLDAQLRLALLYGEEKEYAKAIDQLTQVLKARPTELK
                     IRDYLGFLYEESKDTKKAIETYTFNIQLEPSYFEGQLHLGLLYYRLKQYPEAITHLTQ
                     ARKINPKQPETHIVLGLIYLQKEQFDDAVNVLEEGISQNPKSADLYFNLGTVYDKLNR
                     FDDVEHVMETAINLDPHHADALNYLGYSYAERGVKIDQALSLTKRAVSLKPSNGYYID
                     SLAWALFKSGSFTEALAEMKRAVALAGDDPVLYEHLGDIYAKQRNLTEAREAWLRALE
                     LDPSNNKLMDRFRELGMGDPAQEERIQQAKQRVSEKIQTQ"
     gene            complement(2280..>2746)
                     /locus_tag="VN444_07405"
     CDS             complement(2280..>2746)
                     /locus_tag="VN444_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HWR21670.1"
                     /translation="TGGITVQQMRGKARRLKAERGLDLLIVDYLQLMQGRSDSESRQQ
                     EISDISRSLKALAKELNVPVVALSQLSRAVEARKPPVPMLADLRESGAIEQDADVVMF
                     IYREEVYDQNSERKGIADILVSKHRNGPIGKRELFFHDRFAKFESLDKHEVV"
BASE COUNT          591 a          752 c          741 g          662 t
ORIGIN      
        1 tgtaatatca aatggttacg attaccttct tcttgatgga attctttgtt tggcgtgcgt
       61 ctttccagct tttgccctga tctgccaata tttgtcgcct gtcgaacggg acttggcccc
      121 ctacagttcc cactccgatt ataaagatat tcgaacgtcc tccccatcgc attgggggag
      181 cgatccatgg aaacgagcat tgagcgcggg agaacaggac tggatcggtc tgtcgatctt
      241 gaagagagga cttatgacga acgccctgga cgagattgat gtcgaagcta gagaaacgag
      301 gattgtagga gggctgctgg ctgtcgtgaa atacgaagga atctgttact gagtttgtat
      361 cttctcggat acacgctgtt tggcctgttg gatacgctct tcctgtgccg gatctcccat
      421 ccccaactcg cgaaatcggt ccatcaactt gttattggag gggtccagtt ccaatgctcg
      481 gagccaggcc tcgcgggcct ctgtcaagtt tcgttgcttg gcataaatgt caccgaggtg
      541 ttcatagagc accgggtcat cccctgcgag cgcaacagcg cgcttcatct ccgccaaggc
      601 ctcggtgaac gagccggatt tgaacagggc ccaggcgagg ctatcgatat aatacccatt
      661 actcggtttg agcgagacgg cccgcttcgt tagggaaagc gcctggtcga tttttacccc
      721 ccgctctgcg tagctatatc ccagatagtt gagggcatcg gcatggtggg ggtccagatt
      781 aattgcggtt tccatgacat gctcgacgtc atcgaatcgg ttgagcttgt cgtagactgt
      841 cccgagattg aagtacagat cggcgctctt gggattctgg ctgatgccct cctccaagac
      901 atttactgcg tcgtcgaact gttccttctg cagatagatc aaacccagca cgatgtgggt
      961 ttcaggctgt tttggattaa tcttacgggc ctgggtcaag tgggtgatgg cctccggata
     1021 ttgcttcaat cgatagtaca acaggccgag atgcaactgc ccttcgaagt aggagggttc
     1081 aagttgaatg ttaaatgtgt aggtctcgat ggccttcttc gtgtccttgg actcctcgta
     1141 gagaaatcca aggtaatctc tgattttaag ctccgtcggg cgcgccttga gaacttgggt
     1201 cagctgatca atggccttgg catattcctt ctcctcacca tatagcagcg ccaatcgaag
     1261 ctgggcatcg aggtcagacg ggtcttccgc caacagatca ttcaattctt tcctggcacc
     1321 ctgatagtcc tttgccgcca tatagacacg aaccaactgg tgtcggatag cgcggtttcg
     1381 agggttcaca gtctgaaggt atttccgtaa tacggcgatc gcgcgatcct gtgattgccg
     1441 agattcataa accgaagcga gcgcatgata ggccggttca aacgaagcat tcgccgcaat
     1501 cgaccgctca aaactcgccg cggcctcttc ccctttcccc gcatcgagga gcagtcgtcc
     1561 caagtgataa tggcccacag cagagtcagg agacttcgcc aaaccagtcc gaatagactg
     1621 ctccgcttca ggctgccgct tcagattcag gagcaacagg cccttcgtaa aatagggatc
     1681 cccagacgtt ggatccgcag cgatcgcgcg atctaacagt ttcaccgcac gctccccttg
     1741 ccccgcactg gctaaaatac ctgccatctg ggtgagcagc tgaccatctt ggcttccccc
     1801 ctctgccact tggtccgcat agaggagcgc gttgggcata tcgccaaggg aaaaatagag
     1861 gcttgccagc cgggccttca cagtttgaga tgccggatcc gccttcagcg cagcctggta
     1921 ttctcgaatc gcacgatcca tgtcctgcgc aagctcagct tggtatccca gcatgaagtg
     1981 ataggaagcg ccggcatcgg ggggatgaac cgaggacgca gccttcggcg ggtcggcaag
     2041 agcgagcttc tcaggcatct gaggcgcggc ggcacaggcc accatcatca gggagatcag
     2101 caaacagagc gacgtgagac ctcggctgtt aactgttcgg ctcggaatga ctggcatcgt
     2161 gaccattcgc actatcctcg tggtggactc agagtaacta ctcaggtgtt caggctaaaa
     2221 gccttcagcc tatctacctg cgagctctga aagattatac gaaaggggtt tgggcagggt
     2281 caaacgactt cgtgtttgtc gaggctttcg aacttcgcga aacggtcgtg gaaaaagagt
     2341 tccctcttcc caataggccc gttgcggtgt ttgctcacga gaatatcggc aatgcctttc
     2401 cgttctgagt tttggtcata cacctcttcg cggtatatga acataacgac atctgcatct
     2461 tgctcgatcg cgccgctctc gcggaggtca gccagcatcg gaaccggtgg cttcctggcc
     2521 tctaccgcac gactgagctg cgacaacgcg acaaccggga cgttgagctc cttggccagg
     2581 gcttttagcg agcgtgaaat atcagagatt tcttgctggc gggactccga atcgctccgt
     2641 ccctgcatca gctggagata gtcgacgatc aaaagatcca acccccgctc ggctttcagc
     2701 cggcgcgcct taccccgcat ttgctgtacg gtgatgcctc ctgtat
//
LOCUS       DATMXI010000384         5440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_112376, whole genome shotgun sequence.
ACCESSION   DATMXI010000384 DATMXI010000000
VERSION     DATMXI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112376"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..509
                     /locus_tag="VN444_07410"
     CDS             312..509
                     /locus_tag="VN444_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21671.1"
                     /translation="MCPLSGRLAAGTSDEWFLNCGHRVLASRLRRHKIPFEFEEFDGG
                     HMGVGYRVIDSLRRITAALRR"
     gene            781..2199
                     /gene="icd"
                     /locus_tag="VN444_07415"
     CDS             781..2199
                     /gene="icd"
                     /locus_tag="VN444_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWR21672.1"
                     /translation="MIERCEGLQPPKEGCAIAVKDGQLIVPDDPVIPFIEGDGTGRDI
                     WRASVRVFDAAVKRAYGGKRRIIWFEVYAGEKAFNALKTWLPEETIEAFRTYVVGIKG
                     PLTTPVGGGIRSLNVALRQLLDLYVCLRPVRYIRGVPSPVKRPELVDMVIFRENTEDI
                     YAGIEWEAGSPEVSKVIHFLQDRMGVKKIRFPESSAIGIKPVSREGSERLIEAAIQYA
                     LRHKRKSVTMVHKGNIMKFTEGAFRDWGYALAAGKYHHETISLRESWILDNKEKKPTL
                     TVEANALAIEPGYKDMTPDRQAKIREEVEAALALWPTHGDGQWKRKLMIKDSIADITL
                     QQTLTRADEFDVIATLNLNGDYLSDAIAAQIGGIGIAPGGNINYRTGHAIFEATHGTA
                     PKYADMDKVNPGSVILSGEMMLRHLGWNEAADLVVRGLEETIARRQVTYDFARLMRAE
                     GATDVKELKCSEFATAVIENMG"
     gene            2304..3233
                     /gene="mdh"
                     /locus_tag="VN444_07420"
     CDS             2304..3233
                     /gene="mdh"
                     /locus_tag="VN444_07420"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWR21673.1"
                     /translation="MRPKVTVVGGAGNVGSTVGQYLVAKELADVVLIDIMESVQHGRG
                     LDLLQTGPVLGSDSRIIGTKDYRDTAHSDIVVVTAGIPRKPGMSRDDLLHTNARIVGD
                     VVEQVAVHSPNCILIIVSNPLDAMTQLALTQSGFARERVIGMAGVLDAARFRTFIAQE
                     LRVSVENVHACVLGGHGDAMVPLPRFSTVAGIPITELLSPDRVEALVKRTAGGGGEVL
                     ALLGTGSAYYAPGASVVEMVEAIVKDKKKILPCCVYLEGEYGIHGLCVGVPVKLGAAG
                     VEQVMEIRLTPDETAALHRSAAGVKELVDILKL"
     gene            3456..3728
                     /locus_tag="VN444_07425"
     CDS             3456..3728
                     /locus_tag="VN444_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21674.1"
                     /translation="MMQLRSRYLGSLVLAVGLLVAGCGQEIKKENEQLKGQVTTLQKE
                     NADLKAEAVTLKGESEAMKKELDEMKTQMQLMEEQMKAKGRPAKKK"
     gene            3810..4514
                     /locus_tag="VN444_07430"
     CDS             3810..4514
                     /locus_tag="VN444_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HWR21675.1"
                     /translation="MAATARREIWWLQPMLIVLTLGGFMAYTTWAVLQGAHYDYKNYL
                     SPFYPPFAKPEWWPLSPAFLVLWAPAGFRLSCYYFRKAFHRSFLLAPPACAVADARAG
                     YTGESRFPLFLMNFHRYFLYLAMVELIILWYHAARSVVFDGRLGAGTGSLVMVANVAL
                     LSLYLTSCHSFRHLIGGRLDCFSRCPSRHAIWNQVSRLNERHMLWFWLSLFSVGLTDL
                     YIRLLSMGIISDMRLF"
     gene            4514..>5440
                     /locus_tag="VN444_07435"
     CDS             4514..>5440
                     /locus_tag="VN444_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR21676.1"
                     /translation="MAREQYETYEHDVLIIGGGGAGLRAAIEAKAHGVSVGLICKSLL
                     GKAHTVMAEGGIAAALGNVYPEDNWQVHFRDTMRGGKLLNNWRMAQLHAQEAPERVLE
                     LERWGAVFDRTKDGLILQRDFGGHRYARLAHVGDRTGLEMLRTLQQHAVAQGIEVYME
                     CTVTRLLKDGDRVSGAFGYRRSTGQFIVVMAKAVILATGGIGKLWKFTSNSWECTGNG
                     TMLALDAGTDLIDMESYQFHPTGMVWPPSVRGTLVTEGVRGDGGTLRNNSGERFMFRY
                     IPEFFKAETADNEAEADKWYTDKKHNRRTPDLL"
BASE COUNT         1129 a         1474 c         1725 g         1112 t
ORIGIN      
        1 aaaataggga cagacactat tatcgtgacc attcgtggtg ctcgcggtca tgatcgtgcc
       61 tgctatggat tagccgaacc gttgcatccg gccacccaat caccggcccc tgctgttcga
      121 tactcatatc cattgacaag caaaaacagc cctcctatag ttccggccac cgaaagcaat
      181 gccttctcaa acaacgcggc aagcgaacga gattgtggag gaaaacatgc ctgccatccc
      241 ggaacgacaa gccctgcgcc aggttgtcgt cgacagcctc tgcttgcgcg ataaccccct
      301 cggcgatccg catgtgcccg ctttccggtc gtctggctgc cggcaccagc gacgagtggt
      361 tcctcaattg tggccatcgg gtcctggcga gccgcctgag gcgccacaag attccgttcg
      421 agttcgagga gttcgatggc ggccacatgg gtgtcggtta ccgcgtcatc gactcgttgc
      481 gccgcatcac tgccgcgctg cgccgttagc gattgggcgt tcaatcggga cagccggctt
      541 tcgcggggcg ttcacgatca ttccgtgaca cggtcgtggg cgtcgagcac tatggcttga
      601 cacccctctg gcgttcgtgt tagcgtgcgc ctgtcaagag ttggaaggtc ttcgtgagtc
      661 ctctcttcgc gtgttcctca cacataatct tctggtaaca ttggcgggtc gaaggaccta
      721 ttacgtctgc ttgcgaacga ggccggtgta cgagaaagga tgctggtcag ggagaggtcg
      781 atgatagagc gatgtgaagg gcttcagcca ccgaaagagg gctgcgcgat tgccgtgaaa
      841 gatggccagt tgatcgtccc tgacgatccg gtcattccgt tcatcgaagg tgacgggaca
      901 ggccgcgaca tctggcgcgc ctcggttcgc gtattcgatg cggcggtgaa aagagcctac
      961 ggcgggaagc gccgtatcat ctggttcgag gtctatgcgg gcgagaaggc gtttaatgcc
     1021 ctcaagacct ggttgccgga ggagactatt gaagccttcc gcacgtacgt ggttgggatc
     1081 aagggaccac tcacgacgcc agtgggcggc ggtatccgga gcctgaacgt ggcgcttcgc
     1141 caacttctcg acctctatgt ctgtctcaga ccggtccggt acattcgcgg cgtcccaagc
     1201 cctgtgaagc gtcccgagtt ggtcgatatg gtgatcttcc gagagaatac agaggatatc
     1261 tacgccggga tcgagtggga agccggatcg ccagaggtca gtaaggtcat ccacttcctt
     1321 caggacagga tgggcgtcaa gaagatccgc ttcccggaga gctctgcgat cgggattaaa
     1381 ccggtcagtc gagaagggag tgagcggctg atcgaagctg cgattcaata cgcgctccga
     1441 cacaagcgaa agtccgtgac gatggtccac aaggggaaca tcatgaagtt tacagagggc
     1501 gcctttcggg actggggata tgccctggcg gccggcaagt accatcacga gacgatcagt
     1561 cttcgagaga gttggatcct cgacaacaag gagaaaaagc ccactcttac ggttgaggcg
     1621 aatgccttgg ccatcgaacc cggctacaaa gatatgacac cagaccggca ggcgaagatc
     1681 cgcgaggagg tcgaggccgc tcttgcgctg tggccaacgc atggtgatgg gcagtggaag
     1741 cgcaagctga tgattaagga ctcgatcgcc gatattacgc tgcagcagac cctgacacga
     1801 gccgatgagt tcgatgtgat cgcgacgctg aacctgaatg gcgactatct ctcggatgcc
     1861 atcgccgccc agattggcgg gatcggaatt gctcccggag ggaatatcaa ctatcgtacc
     1921 ggtcacgcaa tctttgaggc gacccatggc acagcgccga aatacgccga tatggataag
     1981 gtcaatcctg gctcggtcat cctgtccggc gagatgatgc tgcggcacct cggctggaac
     2041 gaggcggcgg acctggtggt ccgggggctg gaggagacca ttgcccggag gcaggtcacc
     2101 tatgactttg cccggctgat gcgagctgag ggggccactg acgtcaagga gctgaagtgc
     2161 tccgaattcg ctacagccgt tattgagaac atgggctagg cagggtacga gcagttcatc
     2221 gtttatagtt tatagttcag agaagaggcc gaagagcgta tctatgaact ttgaactatg
     2281 aaccctgaac tggagggggg ctgatgcgac cgaaagtaac cgtagttggc ggtgcgggca
     2341 acgttggatc caccgttggt cagtatctcg ttgcgaaaga gctggccgat gtggtgctga
     2401 tcgatatcat ggagagcgtc caacatggac gggggctgga cctgcttcag acgggtccgg
     2461 ttctgggctc tgacagccgt atcattggca cgaaggacta tcgggacacc gctcactccg
     2521 acattgtggt tgtgacggcc ggtattccca gaaagccggg gatgagtcgg gatgatctgc
     2581 tccatacgaa cgccaggatc gtaggcgatg tggtcgagca ggttgcggtc cactcgccga
     2641 actgtatcct catcatcgtg agtaacccgc ttgacgcgat gacccaactg gcgctcacgc
     2701 aaagcggttt tgctcgagag cgggttatcg gtatggcggg cgtgctggac gccgcgcgat
     2761 tccggacctt tatcgcacaa gagcttcggg tctctgttga gaatgtgcat gcctgcgtac
     2821 tcggcggcca tggcgatgca atggttcccc tgcctcgctt ttccacggtt gccgggattc
     2881 cgatcaccga gttgctgtcg ccagaccggg tcgaggcgct ggtgaagcgg acagccggtg
     2941 gtggcgggga ggtcctggcg ctgcttggaa cgggaagcgc gtattacgcg ccgggagcct
     3001 cagtcgtgga gatggtagag gcgattgtca aggataagaa aaagatcctg ccctgctgcg
     3061 tctatctgga aggggagtac gggatacacg ggttatgtgt cggtgtcccg gtcaagctag
     3121 gcgccgccgg cgttgaacag gttatggaaa tccggctgac acccgatgag acggctgctc
     3181 tgcaccggtc ggcggccgga gtgaaagagc tggttgacat tctgaagctc tgaactggta
     3241 actactcagg tagataggct gtaggctgtt agactgtagg aacacgccat gcgagaccat
     3301 accaagctca gagcgtttga actagccgac gatgtgcgcc ttgattcggt ccgtgcgcaa
     3361 cgactaacag ccttcagcct atagcctaaa cacctgagta gttgcctgaa ctgtagacgg
     3421 taaactgtga actcttaata ggaggaggat gagccgtgat gcaactgaga agcagatatc
     3481 tgggttcctt ggtgctggct gtagggctgt tggtcgcagg ctgcgggcag gagatcaaga
     3541 aggagaatga gcaactgaaa gggcaggtga caacccttca gaaggagaat gccgacctga
     3601 aagctgaggc ggtgacgttg aagggtgaga gcgaggcgat gaaaaaggaa ctcgatgaga
     3661 tgaagacgca gatgcaactg atggaggagc agatgaaggc caagggccgg cccgcaaaga
     3721 aaaagtagcc tgcccaggcg atagtacaac cgttggcgcc tcagagactc atggccgacg
     3781 agcgactcaa caaccagcgg gggtaggtcg tggcagcaac cgcgcgaagg gagatctggt
     3841 ggctacagcc gatgctgatc gtgctgacgc taggcgggtt tatggcctac acgacctggg
     3901 cagtccttca gggagcccac tacgactaca agaactacct gtctcctttc tatccaccgt
     3961 tcgccaagcc tgaatggtgg ccgctttcac cggccttcct ggtcctctgg gccccggcgg
     4021 gttttcggtt gagctgctac tacttccgga aggccttcca tcgctctttc ttgctggccc
     4081 cgccggcctg cgcggttgct gacgcgcggg ccggctatac aggagaatcc cgcttccctc
     4141 tgttcctcat gaatttccat cgttactttc tctacctggc catggtcgag ctaatcatct
     4201 tgtggtatca cgccgcccgc agtgtcgtct ttgacggccg gctcggcgcg gggacgggct
     4261 cactcgtgat ggtggctaat gtcgctctcc tgagtctcta cctgacctct tgccactcct
     4321 ttcgacacct catcggcggg cggcttgact gtttctcgcg ctgcccatcc cggcacgcca
     4381 tctggaatca ggtgagccgg ctcaacgagc gtcacatgct ctggttctgg ctcagcctct
     4441 tttccgtagg attgactgat ctgtacattc gcctcctgtc gatgggcatc atcagcgata
     4501 tgagactgtt ctgatggcgc gggagcagta cgagacgtac gaacacgatg tgctcatcat
     4561 cggtggcggt ggcgcaggcc tgcgcgcagc catcgaagcc aaggcgcatg gcgtctccgt
     4621 gggtcttatc tgtaagtcgc tcctgggcaa ggcccatacg gtcatggctg agggcggaat
     4681 cgcggccgcc ctcggtaacg tctatccaga ggacaactgg caagtgcatt tccgcgacac
     4741 gatgcgaggc ggcaagctgc tgaacaactg gcggatggct caactgcatg cccaggaagc
     4801 gccggagcgt gtcttggaac tggaacggtg gggtgcagtg ttcgaccgga ctaaggatgg
     4861 gctcatccta caacgcgact tcggaggcca ccgctacgcg cgtctggccc acgtcggcga
     4921 tcggactggc cttgagatgc tccgcacgct gcagcagcac gcggtggctc aggggatcga
     4981 ggtctacatg gaatgcaccg taacgcgcct gctcaaggac ggcgatcgtg tgagcggcgc
     5041 gttcggctat cggcgcagca ccggtcagtt catcgtcgtg atggcgaagg cggtgatcct
     5101 tgcgaccggc gggatcggca agctatggaa gttcacctcc aactcctggg agtgtaccgg
     5161 caacggtacc atgctggctc tcgatgccgg cacggacctg atcgacatgg agtcgtacca
     5221 gtttcacccg acagggatgg tgtggccgcc ctcggtgcgg gggaccctcg tgactgaagg
     5281 ggtacgtggc gacggcggga cactgcggaa taacagcggc gagcgcttca tgttccgata
     5341 catccctgag ttcttcaagg ccgagaccgc cgataatgag gctgaggccg ataagtggta
     5401 tacggacaag aaacacaacc ggcgtacgcc cgatctgctg
//
LOCUS       DATMXI010000385         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_272433, whole genome shotgun sequence.
ACCESSION   DATMXI010000385 DATMXI010000000
VERSION     DATMXI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272433"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..129
                     /locus_tag="VN444_07440"
     CDS             1..129
                     /locus_tag="VN444_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWR21677.1"
                     /translation="MIVIEAMKMENELKASGPGVIKEIKVREGDGVSGGDVLVVIE"
     gene            244..1896
                     /locus_tag="VN444_07445"
     CDS             244..1896
                     /locus_tag="VN444_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWR21678.1"
                     /translation="MKRLKCEFDDWQANVLEPALTRTPERKKEFRTSSCIEVKRLYTP
                     LDLADHDYLERLGFPGSYPYTRGVRPTMYRSRPWTMRQYAGYGVAEETNRRFHFLLEQ
                     GQTGLSVAFDLPTQLGYDADDPMAAGEVGKVGVAIDSLADMETLLRGIPLDRVSVSMT
                     INATAAILLAMYVAVAKRQDVAPGQLAGTIQNDILKEYIARGTYIFPPGPSMRLVTDT
                     VAYCAAHLPRWNAISVSGYHIREAGSTAVQEVAFTLADGIAYVEAAIAAGLDIDRVAS
                     QLSFFFNAHNDLLEEVAKFRAARRLWAGMMRRRWGARDPRSWMMRFHAQTSGVSLTAQ
                     QAENNVVRVALQALAAVLGGVQSLHTNSRDEALGLPTEDAALLALRTQQIIAYESGVA
                     NTIDPLGGSYYLEALTDRIEREAAAYVEKIDAMGGMLRAIEVGFIQREIQEAAYREQL
                     ATEERQRIVVGVNDFTTTDPVHIPVFAVDPKVEMEQRAKLDRLRRSRDNSNVERVLHA
                     VGEAARGSENVMPFLIEAVEAYATIGEICAVLRRVFGEHRGS"
BASE COUNT          419 a          531 c          640 g          410 t
ORIGIN      
        1 gtgattgtca ttgaggcgat gaagatggaa aacgagttga aagcttctgg gcctggtgta
       61 atcaaagaga tcaaggtgcg agaaggggac ggtgtcagtg gaggcgatgt cctggttgtg
      121 attgagtaat agcccatgct atcagctttc agctcttctg atacgttccc cgctgtgtct
      181 gagactgacg gctgactgct aaaagctatt ttctgagtaa gcgatgtgga cgacgaagag
      241 cgaatgaaaa ggcttaaatg cgagttcgat gactggcagg cgaacgtcct tgagcctgcc
      301 cttacgcgca ccccggagcg gaagaaagag tttcggacaa gttcctgcat cgaggtgaag
      361 cgcctctata ccccactgga tctcgccgat catgactatc tggaacggct cggatttcca
      421 ggcagctatc cgtatacccg tggggtgagg ccgacgatgt atcgaagccg cccctggacc
      481 atgcggcagt acgccggcta cggagttgca gaggagacta accggcggtt ccactttcta
      541 cttgagcagg gccagacagg gctgtcggta gccttcgatc ttcccaccca gctcgggtat
      601 gacgcggatg acccgatggc cgccggcgag gtgggcaagg taggcgtcgc catcgactcc
      661 ctggccgata tggagacgct gctgcgaggc attcccctcg atcgggtgag tgtctcaatg
      721 accatcaatg cgactgcggc catcctgctg gcgatgtatg tggccgtggc caagcgacag
      781 gatgttgcgc caggacagct tgcggggacc attcagaacg atatcctcaa ggaatatatt
      841 gccaggggaa cctacatctt tccgcctgga ccttcgatgc gtctggtgac cgatacagtt
      901 gcgtactgcg ccgcgcatct tccgcgctgg aacgcgatca gcgtcagtgg gtaccatatt
      961 cgggaggcag gctcgacggc agtccaggag gtagccttca cgctggccga cggcatcgcc
     1021 tatgtggagg cggcgatcgc cgctgggctg gatatagatc gcgttgcctc gcagctttcc
     1081 tttttcttca acgcccataa cgatctcctg gaggaggtag ccaagttccg ggcggctcgt
     1141 cgcctctggg ctgggatgat gcggcggcgg tggggcgcgc gcgacccgag gtcgtggatg
     1201 atgcgctttc atgcgcagac ttccggcgtg agcctcacgg cgcaacaggc tgagaacaat
     1261 gtagttcgcg tggcattgca ggctctcgct gcagtccttg gcggggttca gtcgctgcac
     1321 accaactcgc gcgacgaggc gctgggactt cccaccgaag acgcagccct cctcgcgctt
     1381 aggacccagc agatcatcgc ctatgagagc ggggtggcga acaccatcga tccgctcggc
     1441 ggctcctact atctggaagc cctgaccgat cggatcgagc gagaggctgc cgcctacgtt
     1501 gaaaaaatcg acgcaatggg agggatgctt cgggcgatcg aggtcggatt catccaacgg
     1561 gaaatccagg aggctgcgta ccgggaacag ttggctacgg aggaaaggca gcgcatcgtg
     1621 gttggagtaa acgacttcac taccaccgat cctgtccaca tccccgtctt tgctgtcgat
     1681 ccaaaggttg agatggaaca gcgggcgaaa cttgaccggc tccgtcgtag tcgagataac
     1741 agcaatgtgg agcgggtcct ccatgcggtg ggtgaggcgg cgagagggag cgagaacgtg
     1801 atgccgttct tgatcgaggc agtcgaggcc tacgctacca ttggggagat ctgcgccgta
     1861 ttgcggcgcg tcttcggaga gcatcgtggt tcatagttca cagttcatgg ttcatagatg
     1921 aaaaagcgca gactgcggct tgaacttctc tgaactatca acgttcatgg agggaggcac
     1981 aggcaaaagc tgtgcgagag
//
LOCUS       DATMXI010000386         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_139500, whole genome shotgun sequence.
ACCESSION   DATMXI010000386 DATMXI010000000
VERSION     DATMXI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139500"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VN444_07450"
     CDS             complement(<1..340)
                     /locus_tag="VN444_07450"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWR21679.1"
                     /translation="MVGLCVNVDHIATIRQARRVQEPDPAQAVVLAELAGASGITVHL
                     REDRRHIQDRDVEILRRLVKTKLNLEMAATEEMIQIALALKPEMATLVPERREELTTE
                     GGLDVHGHVDE"
     gene            complement(421..1770)
                     /gene="glmM"
                     /locus_tag="VN444_07455"
     CDS             complement(421..1770)
                     /gene="glmM"
                     /locus_tag="VN444_07455"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWR21680.1"
                     /translation="MRKLFGTDGIRGVANREPMTPETMVKVGRATAHLLRGTSERPAI
                     VIGKDTRLTGYMLETALTAGITSMGVDVLLVGPLPTPGIAFMTRSLRADAGVVISASH
                     NSYEDNGVKFFSHDGLKLPDAMEQRIEELISNGEIDGIRSTPREIGKAYRVGDAVGRY
                     IEFAKNTLPKGTTLKGMRVVVDCANGAAYKVSPAVLKELNAEVVSLNVQPDGTNINKG
                     CGSLHPGGLQRAVSRHKAHLGFALDGDADRVLFVDEQGETMDGDHILALCALDLKREG
                     RLREDTIVATVMSNIGLDIAMQEAGIKVVRTAVGDRYVLEEMLAKRYMLGGEQSGHII
                     FLEHQTTGDGIVTALQVLATMQRCGKPLSELKACMRSYPQVLINTPVRRRAAIEELPR
                     VQEAIQLAEAGIGVRGRVLVRLSGTEPVARVMVEGEDPAKVERLAREIALVIEKELG"
     gene            complement(1900..>2074)
                     /locus_tag="VN444_07460"
     CDS             complement(1900..>2074)
                     /locus_tag="VN444_07460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWR21681.1"
                     /translation="SRKSLVGNVLNLPVEQRQYGTAACVAAAVLQGAAFVRVHEVRSC
                     AHLVRMLDAIRRA"
BASE COUNT          377 a          731 c          537 g          429 t
ORIGIN      
        1 cctcatcgac gtgtccgtga acgtccagtc ctccttcagt ggtgagttcc tcacgccgtt
       61 ccgggacaag cgtcgccatc tccggcttga gcgccagggc gatctggatc atctcttccg
      121 tggctgccat ctccaggttc agcttcgtct tgaccagccg ccgcaggatc tcgacatccc
      181 gatcctggat atgccgccgg tcctctcgta ggtgtacggt aatcccggac gcgccggcca
      241 gttcggccag cacgaccgcc tgagctggat caggctcctg cacccgtctc gcctgcctga
      301 ttgtggcgat atgatcgacg ttcacacaca acccaaccac ggctcactcc gttcgctgtc
      361 gcaggtctat ctggtcgtct ggtccgacca aacggacgaa acagaccaat aggcccgctt
      421 ttagcctagt tctttctcaa tcaccagcgc gatctctcgg gctagccgct cgaccttcgc
      481 tggatcctcg ccttctacca tcacacgggc caccggttcc gtgccggata agcgaaccag
      541 gacccgtccc ctgactccta tacccgcctc cgccaattga atcgcctcct gcacccgtgg
      601 aagctcctca atggcagctc tgcgccggac gggcgtgtta atcaatacct gaggatacga
      661 gcgcatgcac gccttcagct ccgaaagcgg tttcccgcac ctctgcatag ttgccagcac
      721 ctgcagggca gtcacaatcc cgtcgccggt ggtctggtgt tccaggaaga tgatatgccc
      781 ggactgctcg ccccccagca tgtagcgctt cgccagcatc tcctccagaa cgtaccgatc
      841 gcccaccgcg gttcgaacga ccttgatccc cgcctcctgc atcgcgatgt ctagtccgat
      901 gttgctcatc acggtggcca caatcgtgtc ttctcgcaga cggccttccc gcttgaggtc
      961 gagggcacag agggcgagga tatgatcgcc atccatcgtc tcgccctgtt catccacaaa
     1021 cagcacccgg tccgcgtccc cgtcaagggc aaacccgagg tgtgccttgt gcctgctgac
     1081 cgctcgctgc aaccctcctg gatgcagtga gccgcagcct ttattgatat tggtgccgtc
     1141 gggctggaca ttcagcgaca ccacctcggc gttcagctct ttgaggactg cgggcgacac
     1201 cttgtaggcg gcgccgttgg cgcaatcaac caccacccgc atccctttca gcgtcgttcc
     1261 cttgggaaga gtatttttcg caaattcgat atagcggccg acggcatcgc ccactcggta
     1321 ggccttgccg atctcacggg gcgtcgacct gatcccgtcg atctctccat tagagatcag
     1381 ctcctcgatc cgctgctcca tcgcatccgg cagcttcagg ccgtcgtgag aaaagaactt
     1441 gacgccgttg tcctcatacg agttatggga ggccgagatc accactccgg catcggctcg
     1501 aaggcttcgc gtcataaagg cgatcccagg cgtcgggagc gggcccacca atagcacgtc
     1561 cacgcccatc gaggtgatgc cggcggtcag cgccgtctcc agcatatagc cggtcagacg
     1621 cgtgtccttc ccaatcacga tcgcgggtcg ctcgcttgtc ccgcgtaaca ggtgagccgt
     1681 tgcgcgcccc accttgacca tggtctccgg ggtcatcggc tccctgttgg ctacaccccg
     1741 aatgccgtcg gtgccgaata gtttccgcac aaccactcct tttccctcga tgcgcctcgc
     1801 caccgctcac ctcgctcctt ggtccgacca accggacacg atcagcacat ctacggcgtc
     1861 aatccgcttg aattgggcaa ttgtggtcca acatggccgc tacgccctcc tgatcgcgtc
     1921 cagcatcctg accagatgcg cacaggaccg gacctcgtga actcggacaa aggccgcacc
     1981 ctgcagcacc gccgccgcga cacaggccgc cgtaccgtat tgacgctgct caacaggaag
     2041 attcagtaca ttcccaacga gcgacttacg tgat
//
LOCUS       DATMXI010000387         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_43485, whole genome shotgun sequence.
ACCESSION   DATMXI010000387 DATMXI010000000
VERSION     DATMXI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43485"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2558)
                     /locus_tag="VN444_07465"
     CDS             complement(114..2558)
                     /locus_tag="VN444_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR21682.1"
                     /translation="MGETMASPLKSDTLRKICPPESLPFEGTDELKSLEEVVAQDRAV
                     QAITFGVGIRSEGFNLFVLGPAGTGKTTAIKRFLASEAAKLPTPPDWCYVNNFADPQR
                     PRALCLPPGRAHLFKADCERLLEELKTGVPRAFESEAYEQNRHTILEELQKQTSDELE
                     ALGKRAETLGFGLAKTASGFMIIPMFRGKPLKPEGFEGLDEETKRQVNLRSEEVQKDL
                     AATRRRIRDLEREAKNRLDGMDREVATSAVDHLVEEVKERYVDHATVQAYLEAIREDV
                     IANVDLFRRDQNAAQGPPELTAMLRQGLDPFDRYRINVLIEHGEDGGAPVVVEPHPTC
                     QSLVGRIEHQVHMGALYTNFLMAKGGALHRANGGFLVLEAMELLKQFFAWDQLKRTLK
                     NRRIKIEEPGEQFHLYSTVTLEPEPIPLNVKVVLIGSPWLYYLLHALDPEFLELFKVQ
                     AEFSDRVARTPETILAFARLLATCCEAEGLKAFDRGAVAKLVEHSSRLVEDQERLATT
                     FSHLLDVAREASFLAEQDGHPRVMAEDVRRAIDAKIKRSNRLEERLQELITDRTILVD
                     TDGAVVGQVNGIAIIPLGEYHFGKPSRITARTFLGRGGIIDIEREARLGGRIHSKGVF
                     ILSGYLGGRYVQEVPLALSASLAFEQLYEEVEGDSASSAELYAILSSLSGLPIKQGFA
                     VTGSVNQQGEVQAIGAVNAKIEGFFDVCRARGLTGEQGVLIPASNVRHLMLREDVVEA
                     VAAGQFQIIPVHTIDEGIALLTGLGAGDRGWDGTFPEGSVNALVQGRLIEMAEQARAY
                     SGEPQVSPSVDRHDEL"
BASE COUNT          506 a          853 c          710 g          546 t
ORIGIN      
        1 gccttatcct gtctgtactc cgacattgtc gcttacgctc cgcagttccg ggttccgagc
       61 tccaagtccc gagtgaaaga atgaccccaa cggttgctta gtctgaaaca tcattacagc
      121 tcgtcatggc ggtctacact gggcgagacc tgaggttcgc cgctgtaggc cctcgcctgc
      181 tccgccatct cgatgagccg cccctgcacc agcgcattca cgcttccttc tggaaaggtc
      241 ccgtcccaac cgcgatcacc agcccccagc ccggtaagca aggcaatccc ctcatcgatg
      301 gtgtgaacgg gaatgatctg aaattgcccc gccgcgaccg cctctacgac atcctcccta
      361 agcatgaggt gccggacatt actcgctggg atcaggactc cctgttctcc cgtcagcccc
      421 ctcgcgcggc agacatcaaa aaacccctcg atcttggcgt tgacggcccc gatagcctgg
      481 acctctcctt gctgattaac cgaccccgtc accgcaaatc cctgcttgat cggtagccct
      541 gacaggcttg agaggatcgc atacagctcc gctgaggagg cgctgtcgcc ttccacctct
      601 tcatagagtt gttcgaaggc gagactcgcc gacagggcaa gcggtacctc ctgtacgtag
      661 cggccaccga gatagcctga aaggataaat acgcctttgc tgtgaatacg gccgcccagc
      721 cgggcttcgc gttcgatgtc gatgatgccg ccgcgcccga ggaaggttcg cgccgtgatt
      781 cgactcggct tgccgaagtg atactcccca agcgggataa tggcaatccc attcacttga
      841 ccaaccacgg cgccatcggt atcgacaagg atagtccgat cagtgatcag ttcctgtagc
      901 cgttcctcca gtcgattgga tcgtttaatc ttggcgtcga tagcccgccg cacgtcttcc
      961 gccatgaccc gggggtgtcc atcctgctcg gccaggaagc tggcctctcg ggcgacatcc
     1021 aggagatgac tgaacgtcgt ggcgagtcgt tcctgatcct ccaccaggcg ggagctgtgc
     1081 tccaccagct tcgcaacggc accccgatcg aatgccttga gcccctctgc ctcgcaacag
     1141 gtggccagga gacgggcaaa tgccaggatc gtttcaggag tcctggcaac gcgatcactg
     1201 aactctgcct ggaccttaaa cagctccaga aactccgggt cgagggcgtg caacagatag
     1261 tagagccagg gactgccgat gaggaccacc ttcacgttga gcgggatcgg ctcgggctcg
     1321 agcgtgaccg tgctgtagag gtgaaattgc tccccgggct cttcgatctt gatccgacga
     1381 ttcttcagcg tccgcttcag ttggtcccac gcaaagaatt gcttcaacag ctccatggcc
     1441 tccaagacca ggaagccccc attggcccgg tgcaaggcgc cccccttggc catcaggaag
     1501 ttggtgtaga gggcacccat gtggacctga tgttcgattc gtccaaccag gctctgacag
     1561 gtagggtgag gttcgaccac aaccggcgcg ccgccatctt ctccatgttc gatcaaaaca
     1621 ttgatccgat accggtcgaa ggggtccaac ccttgccgca gcatggcagt cagttcaggt
     1681 ggcccttgtg ccgcgttttg atctcgacga aacagatcca cattggcgat gacgtcctca
     1741 cgtatcgcct cgaggtacgc ctgtaccgtt gcgtgatcta cgtaacgctc tttgacctcc
     1801 tcgaccaggt ggtcaactgc agaggtcgcg acctcccggt ccatcccgtc aagacgattc
     1861 ttcgcctccc gctccaagtc gcgaattcgg cggcgcgtcg cggccagatc cttctgcacc
     1921 tcctcacttc tcagattgac ctgtcgcttg gtctcctcat cgaggccctc aaacccctcc
     1981 ggcttcagtg gcttccctcg aaacatgggg atgatcatga agccgctggc cgtcttggca
     2041 agcccaaacc caagcgtctc cgcacgcttc cccaaggcct ccagctcatc gctcgtctgt
     2101 ttctgcagct cctccaggat ggtgtgacgg ttctgctcat aggcttcgga ctcgaaggct
     2161 ctgggaacac cggtcttcag ctcctccagg agccgctcgc agtcggcctt gaagaggtgg
     2221 gcgcggcccg gcggaaggca tagcgcgcgg ggacgttgcg gatcggcgaa gttattgaca
     2281 tagcaccaat caggaggggt ggggagctta gccgcctcgc tggccaggaa gcgtttgatc
     2341 gctgtggtct tccctgttcc ggcaggccca aggacaaaga gattgaaccc ttcgctgcgg
     2401 atgccgacac cgaaggtgat ggcctggaca gcacgatcct gagctacaac ctcctccagc
     2461 gatttaagct cgtcagtccc ttcgaagggg aggctctccg gagggcagat cttacggagc
     2521 gtatcagatt tcaatggact ggccatcgtc tcacccatct cccttcaact cctcccgaac
     2581 cgaccgcttg gccctcttcc gatcgtagcg aaccc
//
LOCUS       DATMXI010000388         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_227573, whole genome shotgun sequence.
ACCESSION   DATMXI010000388 DATMXI010000000
VERSION     DATMXI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227573"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1428
                     /gene="ffh"
                     /locus_tag="VN444_07470"
     CDS             85..1428
                     /gene="ffh"
                     /locus_tag="VN444_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWR21683.1"
                     /translation="MFEGLTERLSLVLKNLRGHGRLTEENIAEALREVRLALLEADVN
                     FKVVKGFIERVRERAVGREVLESLTPGQQVVKVVYEELVEVLGKTRAPLAYASDHPTV
                     VMLVGLHGAGKTTTAAKLARWFVSEGRSPLLVAADITRPAAREQLQTLGRALGVPVYA
                     GAGSALQVCQEARRLAKERGQNPVILDTSGRLHIDEPLMQELVAIAGATSPTERLMVA
                     DAMTGQDAVNSALRFDADLDLTGFILTKLDGDSRGGAALSIRSVTGKPIKFIGVGEKT
                     DALEPFHPERLASRILGMGDILTLVEKVQARVDQKQALDLVKKVRTEGFTLEDFRLQL
                     QQLKDLGPLDQVMEMIPGLSRQKGLADTISAERDFKQAGAIINSMTRKEREVPEILNG
                     SRRRRVAAGSGTSVADVNRLLKQFIQARKLMKQFIPGGSGARGKMAHRLRSFMGV"
     gene            1456..1737
                     /gene="rpsP"
                     /locus_tag="VN444_07475"
     CDS             1456..1737
                     /gene="rpsP"
                     /locus_tag="VN444_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWR21684.1"
                     /translation="MAVKIKLRRIGAKQHAFYRLVVGDSLAAGGGKVLESLGTYDPHG
                     EPPALSVQAERALHWLQCGAEPTDSARQLLRRAGVMRQFAELRAAGKGQ"
     gene            1777..2061
                     /locus_tag="VN444_07480"
     CDS             1777..2061
                     /locus_tag="VN444_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWR21685.1"
                     /translation="MEGGPSVPSQDTMEEVRALKALVEQMAMALVDSPDQVTVDAAEE
                     DEAFVLRLRVAPPDVGRVIGKQGRTAKAMRTVLHAIAARSKRRTVLEILE"
     gene            2066..2590
                     /gene="rimM"
                     /locus_tag="VN444_07485"
     CDS             2066..2590
                     /gene="rimM"
                     /locus_tag="VN444_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWR21686.1"
                     /translation="MSSPFLVLGRAVKAWGLKGEVKVQPSADSIAIAVGSATVYLRGA
                     GGDLAEYVVERVRPAGSGWIMQFQGVGTIEQAERLVDHDLLIPRSAAPTLPEGTYYHA
                     DLIGLKVVTEEGRELGRIVEILETGANDVYVVHGEGSEWLLPAIREVVRRVDLAGEIM
                     LVRLLKGMIETEAV"
     gene            2577..3317
                     /gene="trmD"
                     /locus_tag="VN444_07490"
     CDS             2577..3317
                     /gene="trmD"
                     /locus_tag="VN444_07490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWR21687.1"
                     /translation="MRRYDILTLFPGFFQGPLSESILKRAQQRGIVQITIHDLRSYAH
                     DRHTIVDDRPYGGGAGMVLKPEPILEAVTSLRQGQETHLVLLTPQGRPFKQAIAQELV
                     EHQHLLCVCGRYEGFDERVRDLLAPDEISIGDYVLTGGELPALVLLDAVIRLIPGVLG
                     DEDSARYDSFVDALLEFPHYTRPQSIQGHGVPDVLLSGDHERIRRWRRKEALRRTKVR
                     RPDLLQQAVLSKEDLDLLGEIEGEQGTG"
     gene            3384..3731
                     /gene="rplS"
                     /locus_tag="VN444_07495"
     CDS             3384..3731
                     /gene="rplS"
                     /locus_tag="VN444_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWR21688.1"
                     /translation="MDVIRSVEAPSLKAQIPDFSPGDTIKVQVKVREGDKERFQVFEG
                     VVIRKRGDGLRETFTVRKVTYGVGVERIFPIHSPMIDKIECTRRGHVRRAKLYYLRKL
                     KGKAARISERKLA"
     gene            3746..4348
                     /locus_tag="VN444_07500"
     CDS             3746..4348
                     /locus_tag="VN444_07500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWR21689.1"
                     /translation="MRPEEVETCLRTAGYHLIAGVDEAGRGSLAGPVVAAAVILPPAC
                     AIDGVDDSKALSAHRREQLDLAIRTEAVAVGFGVIQEEVVDAINILQATMLAMRRAIE
                     ALNPPPDFVLIDGDRSPNCSLPHRPIPSGDRLCFSISAASILAKVGRDRIMQAYDLAL
                     PQYGFGRHKGYGTPEHLSAIARFGASPIHRKSFRGVREYV"
     gene            4361..>4644
                     /locus_tag="VN444_07505"
     CDS             4361..>4644
                     /locus_tag="VN444_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWR21690.1"
                     /translation="MKAGRLGLGAEGERVAKAYLQTQGFRILHENYSTPLGEIDLIAK
                     EGGVVVFVEVKARTSGEFGPPQASVTLAKQRQIVKVARLYLQRERLAEVA"
BASE COUNT          932 a         1230 c         1573 g          909 t
ORIGIN      
        1 agcacctgcg cctctgcgca gttttgacct tgacaaggag cgaggagggg gctattttta
       61 ggcgctgcga agggagtggg agaggtgttt gaaggactca cagaacgatt aagtttggtc
      121 ctgaaaaatc ttcgtggcca cgggcggctc accgaagaga acatcgccga ggcgcttcgt
      181 gaggttcggc ttgctctgct cgaagcggat gtcaatttta aggtcgtgaa agggttcatc
      241 gagcgcgtcc gcgaacgggc agtgggccgt gaggtattag agagtctgac tcctggccag
      301 caggttgtca aggttgtcta cgaagaactg gtagaggtgc tgggcaagac gcgcgcacct
      361 ctggcctatg cttcggatca tcctacggtc gtcatgctgg tcggcctgca cggagcaggc
      421 aagacgacta cggcggcaaa gttggcccgg tggttcgtgt ctgaaggacg gtcgccactc
      481 ctggtggcgg ccgatataac caggccggcg gccagggagc agctccagac gctggggcgg
      541 gccctcgggg ttccggtgta cgctggggcg ggttctgcgc tgcaagtttg ccaggaggca
      601 aggagactgg caaaagagcg cggacagaat ccggtcattc tcgacacctc cgggcgactc
      661 catatcgatg agccgctcat gcaagagctg gtagcaattg ccggggcaac ctccccgacc
      721 gagcggttga tggtcgccga cgccatgacc ggacaggatg cggtgaattc cgcacttcgg
      781 ttcgatgcag atcttgacct gacggggttc atccttacca agctggatgg ggattcccga
      841 ggcggagcgg ccctctcgat caggtcggtg actgggaagc cgatcaagtt catcggtgta
      901 ggcgagaaga ctgatgccct cgagcctttc caccctgaac gactggcttc tcggatcctt
      961 gggatgggcg atatcctcac cctcgtggaa aaggtacagg cgagggtgga tcagaagcaa
     1021 gccctggacc ttgtgaagaa ggttcgcacc gagggcttta cgctggagga ttttcgttta
     1081 caactccagc aactgaagga ccttgggccc ctcgatcagg tgatggagat gatccctggg
     1141 ttgtcacggc agaagggctt ggctgacacc atttcagcgg agcgggattt caagcaagcc
     1201 ggggcgatta ttaattcgat gacccggaag gagcgggagg ttcccgagat tctcaatggg
     1261 agccggcgcc ggcgagtcgc cgccggaagc ggcacatctg tggcggacgt gaatcgtctg
     1321 ctcaaacagt ttatccaggc ccggaaactg atgaaacagt tcattcctgg gggtagcggg
     1381 gcgaggggaa aaatggcaca tcgccttcgg tccttcatgg gagtgtgacg ccggacggat
     1441 gagggggaga taaagatggc tgtgaagatc aaattgcggc ggataggagc gaaacagcac
     1501 gccttctatc gcttggttgt gggagactca ctggccgctg gaggcggtaa ggtgctggag
     1561 tccctgggta cctacgatcc ccatggggag ccgcctgcac tgtccgtaca ggcggagcgc
     1621 gcattgcatt ggttgcaatg cggtgcggag cctaccgaca gcgccaggca gcttttgcgg
     1681 cgggcaggcg tcatgcggca attcgctgag ctgagagccg caggaaaggg tcagtaaccc
     1741 tgggattgtt gggccaaggt accgggagga ggaaagatgg aaggcggtcc gtcggttccg
     1801 agtcaggata ccatggagga agtaagggcg ctcaaggcac tcgtggaaca aatggccatg
     1861 gcgctggtgg acagcccgga ccaggtgacc gtcgatgcgg cagaggaaga tgaggcgttc
     1921 gtcctccgcc tgcgagtggc ccctcccgat gtcggcaggg tgatcggaaa gcagggccgg
     1981 acggccaagg caatgcgcac cgtgctgcac gccatcgcag ccagatcaaa gcgtcggacc
     2041 gtcctggaga ttctggaata ggtgaatgtc ctcgccattc cttgtactgg gcagggctgt
     2101 caaggcttgg gggctgaagg gagaagtgaa agtacagccc tccgctgact cgatcgcaat
     2161 tgcagtaggg tcggcaaccg tgtacctcag gggggccggg ggtgaccttg ccgagtacgt
     2221 cgtggagcgg gttcggccgg cgggttcagg ctggatcatg caatttcagg gcgtgggaac
     2281 cattgagcag gccgaacgtc tggtcgatca tgatctgctc attcccagat cggctgcccc
     2341 aacgcttcca gagggaacgt attaccatgc cgatctcatc ggccttaagg ttgtgactga
     2401 agagggacga gagctcgggc gaattgtcga aatcctggaa acgggcgcca acgatgtgta
     2461 cgtggtccac ggtgaaggtt cggaatggtt gctgccggcg attcgagagg tcgtgagaag
     2521 ggtcgatctt gcaggggaga tcatgctggt ccgtctgctg aaagggatga tcgaaactga
     2581 ggcggtatga cattctcacc ctcttccctg ggttctttca gggacctctc tcagaaagca
     2641 tcctcaagcg agcccagcag cgcggcatcg tgcagatcac gatccatgat cttcggagct
     2701 atgcccatga tcgtcatacc atcgtggacg atcggccgta cgggggtggc gccggaatgg
     2761 tgcttaagcc cgagccgatc ctcgaggcgg tcacgagcct ccgtcagggg caagagactc
     2821 atctggtcct cttgaccccc cagggtcgac cgttcaagca ggcgattgcc caggagcttg
     2881 tcgagcacca acacctgctg tgcgtctgcg gccggtacga ggggttcgac gaacgggtga
     2941 gagacctcct ggcgcccgat gagatctcca tcggggacta cgtcctgact gggggtgagc
     3001 tgccggccct tgtgctcctg gatgccgtca tccggcttat tcctggcgtg ctgggcgatg
     3061 aagactcagc ccggtacgac tcttttgtag acgcgttgct ggagtttccc cactatacgc
     3121 ggccacagag tattcagggg catggggttc cggacgtgct gctgtcgggt gaccatgagc
     3181 ggatcaggcg ctggcgccga aaggaggcct tgcggcgtac gaaggtgcgt cggccggatc
     3241 tcctgcagca ggctgtcctg agcaaagaag accttgatct cctgggggag atcgaaggcg
     3301 agcaagggac cggttaagcg attcgatgtt cgaggggcga ggtgcttcgc acctcatact
     3361 ttttgcatgt tggaggctga atcatggatg ttatcaggag cgtcgaagcg ccctcgctga
     3421 aggcgcagat cccagacttt tcgcccggcg acacgattaa ggtgcaggtg aaggtccggg
     3481 aaggggataa ggaaagattt caggtattcg aaggggtggt catccgtaag cgcggcgacg
     3541 gccttcgtga gacattcacg gtgcgcaagg tcacgtacgg cgtcggtgtg gagcgcatct
     3601 tccccatcca ttcccccatg attgacaaga tcgagtgcac aagacggggt catgtccgtc
     3661 gggcgaaact ctactacctg cgaaagctga agggaaaggc tgctcggatc tcggagcgca
     3721 agctggcgta aagaggatcg ctgctttgcg ccctgaagag gtggagacct gcctccgcac
     3781 tgcggggtat cacctcattg ccggtgttga tgaggccggg cgaggatccc tggccgggcc
     3841 ggtagtggct gccgctgtga tcctcccgcc ggcctgcgcg atcgatggag tcgatgactc
     3901 caaggcgctc agcgcacacc ggcgggaaca actcgacctg gcgatcagga ccgaagcggt
     3961 ggccgtcggc ttcggcgtga tccaggagga ggtcgtcgat gccatcaata ttctccaggc
     4021 gaccatgctc gccatgcggc gagccatcga ggctttgaat ccacctcctg atttcgttct
     4081 gatcgacggt gatcgatccc ccaactgttc cctcccccat cgaccgatcc cttcaggaga
     4141 tcgtctctgc ttctccatct ctgcggcttc catcctggcg aaggttggcc gggaccggat
     4201 catgcaagcg tatgacctgg ctctcccgca gtacggcttc ggccggcaca aagggtacgg
     4261 gaccccggaa catctgtcgg ccattgcgcg gttcggggcg agccctatcc acaggaagag
     4321 cttcagaggt gtccgggaat acgtgtaact gggcctatcg atgaaagcag gacggttggg
     4381 tctgggagca gaaggggagc gcgtagcgaa agcgtatctg cagacccaag ggttccggat
     4441 cctccacgag aattattcga ctccgctcgg cgagatcgac ctgatcgcca aggagggtgg
     4501 cgtggtagtc ttcgtggagg tgaaggcccg tacgtcaggc gagttcggtc cgccgcaggc
     4561 ctcggtgacg ctggcaaaac agcgacagat cgtcaaggtg gcgcggctat atttgcagcg
     4621 ggagagactc gccgaggtag cctg
//
LOCUS       DATMXI010000389         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_7641, whole genome shotgun sequence.
ACCESSION   DATMXI010000389 DATMXI010000000
VERSION     DATMXI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7641"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /gene="rplC"
                     /locus_tag="VN444_07510"
     CDS             <1..180
                     /gene="rplC"
                     /locus_tag="VN444_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129899.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWR21691.1"
                     /translation="SSFPSRVFRGHHMPGRMGTDTMTIKGLKVVKVIPEQNLLLVRGA
                     VPGPAGGLVTISKRG"
     gene            185..820
                     /gene="rplD"
                     /locus_tag="VN444_07515"
     CDS             185..820
                     /gene="rplD"
                     /locus_tag="VN444_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWR21692.1"
                     /translation="MAIKVHTLDTNGVKTAEFTLNEAVFDAAAVPQLLHDVVKMQLAN
                     RRQGTASTRTRSDVRGGGRKPWKQKGTGRARAGTRRSPLWRGGGTVFGPKPRSYGYAV
                     PRQVRAAALRAALSEKVRSGQFMVLDGLSLEEPSTKAFMALLEKVGVAGRALIITERV
                     QRDDATSKSCRNLPYLTLLPPEGLNVYDVLRHDILIMTRNAVSVVEEAWKP"
     gene            817..1107
                     /locus_tag="VN444_07520"
     CDS             817..1107
                     /locus_tag="VN444_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWR21693.1"
                     /translation="MKESYQIIRRPLITEKGTDLKEHSNQYLFEVARGANKIEIKRAV
                     ESLFRVKVLSVRTLTVNGKKKRVGRFIGHTPGWKKAVATLKVGESIEFFEGV"
     gene            1108..1926
                     /gene="rplB"
                     /locus_tag="VN444_07525"
     CDS             1108..1926
                     /gene="rplB"
                     /locus_tag="VN444_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWR21694.1"
                     /translation="MPIRAYKPTSAGRRFQTVLVYDDVTKATPEKTLLRPLVKGGGRN
                     SAGRLTVRHRGGGHKRQYRLIDFKRDKAGVPAKVASIEYDPNRSARIALLHYADGEKR
                     YIVGPLGLQVGEQVMSGRDAEVKIGNALPLRKIPVGMTLHNLELKPGKGAQLARSAGS
                     GVQFLAKEGDYGLVKLPSGEVRRISLDCMAVIGQVGNLDYEKVSLGKAGRSRWLGVRP
                     AVRGVAMNPHDHPMGGGEGKSSGGRHPCSPWGKLERKTRKKRKASDRFIMKRRK"
     gene            2018..2302
                     /gene="rpsS"
                     /locus_tag="VN444_07530"
     CDS             2018..2302
                     /gene="rpsS"
                     /locus_tag="VN444_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWR21695.1"
                     /translation="MARSLKKGPYVDPKLLKKIEVMNDGREKKIVKSWSRRSVILPEF
                     VGHTLAIHNGKKFIPIYISENMVGHKLGEFSPTRTFRGHGSHTARSSALK"
     gene            2319..2666
                     /gene="rplV"
                     /locus_tag="VN444_07535"
     CDS             2319..2666
                     /gene="rplV"
                     /locus_tag="VN444_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWR21696.1"
                     /translation="MECRAVGRFIGIPARKARLVVDLIRGYGISQAIDTMKFTKKYAA
                     KVVEKILRSALANAQHNHGAKNVDRLFVKEAFVNQGPTTKRFRPRSMGRANTIRKRSS
                     HITIVLTERESAR"
     gene            2738..3445
                     /gene="rpsC"
                     /locus_tag="VN444_07540"
     CDS             2738..3445
                     /gene="rpsC"
                     /locus_tag="VN444_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWR21697.1"
                     /translation="MGQKVHPIGFRLGFIKPWRSRWFATKGYADALHEDLKFRSFIKE
                     RVYHAGVSGIDIERKADQIRIIIHTARPGIIIGKKGSEVDKLKVALSGMSKKPIQLDI
                     VEVRRAELDAQLIAEQVASQLLRRVAYRRAMKKTVQSSMRLGAQGVQIVCAGRLAGAE
                     IARKERYREGRMPLSTMRADIDFGFTQAKTTYGLIGVKCWVYHGEATPETVAAQPERD
                     APQDRQRRPKGPALVQS"
     gene            3452..3871
                     /gene="rplP"
                     /locus_tag="VN444_07545"
     CDS             3452..3871
                     /gene="rplP"
                     /locus_tag="VN444_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWR21698.1"
                     /translation="MLIPKRVKFRKQHRGRRSGLAIRGSTLAFGAYGLKALEAAWITN
                     RQIEAARRAITHHVKRGGKVWIRIFPDKPITKKPAETRMGKGKGAPEGWVAVVKPGRV
                     LCEMEGVTEATAKEAMRLAAHKLPIATRFVSRTMVAS"
     gene            3888..4109
                     /gene="rpmC"
                     /locus_tag="VN444_07550"
     CDS             3888..4109
                     /gene="rpmC"
                     /locus_tag="VN444_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWR21699.1"
                     /translation="MDAKAFRELSAEELQQKIRDVRDELFKLKLRASAAQLENPARIR
                     RLRREIAIGETVRTESLRKQASPGVERTP"
     gene            4106..>4264
                     /gene="rpsQ"
                     /locus_tag="VN444_07555"
     CDS             4106..>4264
                     /gene="rpsQ"
                     /locus_tag="VN444_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWR21700.1"
                     /translation="MTEQEKKGRRKTLVGVVVSDKMQKTVVVMVERIVRHDAYSKMVR
                     RRTKVKAHD"
BASE COUNT         1012 a         1019 c         1386 g          847 t
ORIGIN      
        1 tcgtcctttc cgtctcgagt cttcagggga caccatatgc cgggacggat ggggacagat
       61 acgatgacca tcaaagggct caaggtggta aaggtcattc cagagcagaa cctgttgctg
      121 gttcgcggtg cagtaccagg gccggcaggg ggcctggtca cgatcagcaa gcgaggctaa
      181 ggcgatggca atcaaggtcc acactttaga tacgaacggc gtgaaaacag cggagttcac
      241 actcaatgag gcggttttcg atgccgccgc ggtgccgcag cttctgcacg acgttgtcaa
      301 gatgcaactc gcgaatcgac gtcaggggac cgcttcaacc cgcacacggt ctgatgttcg
      361 tgggggcgga agaaaaccct ggaagcaaaa ggggactggt cgggctaggg ccgggacgag
      421 acgctcaccc ctgtggcgcg gcggagggac cgtgtttggt cccaagcctc ggagctatgg
      481 ctatgcagtc ccgcggcagg tgcgggctgc ggcgcttcgc gccgcccttt ctgagaaggt
      541 ccggtccggg cagtttatgg tgctggacgg tctctcactg gaggagccaa gcaccaaggc
      601 attcatggcg ctactggaga aggtgggtgt tgccgggcga gccttgatca tcacagagcg
      661 agttcagcga gacgatgcga cttccaagtc gtgccgcaat ctgccctatc ttacgctttt
      721 gccgcccgag gggctgaacg tgtacgatgt cttgaggcat gatatcctta tcatgaccag
      781 gaatgcggtg agcgtggtag aagaggcgtg gaagccatga aagaatcata tcagatcatc
      841 cgacgtcctc ttatcaccga gaaggggaca gatttgaagg agcacagcaa ccagtacctt
      901 tttgaggtag cccgaggtgc gaataagatc gaaatcaagc gtgcggtcga atcgctgttt
      961 cgggtaaagg tactgtctgt gcggacgctt accgtcaatg gcaaaaagaa acgggtaggc
     1021 cgctttatcg gtcatacgcc gggctggaag aaggcggtgg cgaccctgaa agttggggaa
     1081 agcatcgagt tcttcgaggg agtatagatg ccgatccgag catacaagcc gacatccgca
     1141 ggcaggcgct tccagacggt tctggtgtac gatgatgtga ccaaggctac tccggagaag
     1201 acgcttctcc gtccgctcgt caagggaggg ggacgaaaca gtgctggtag actcactgtg
     1261 cggcatcgtg ggggcggaca caagcgtcag taccgcctca ttgattttaa gcgcgacaaa
     1321 gccggggtcc ccgcaaaggt ggcgtcgatt gagtatgatc cgaatcgctc tgcgcgaatc
     1381 gctcttctgc actacgcaga tggggagaag cgatacatcg ttggtccgct tggccttcag
     1441 gtcggtgagc aggtgatgtc cggccgcgat gccgaggtca agatcggcaa cgccctccca
     1501 ttacgcaaga tcccagtcgg catgacgctt cataaccttg agttgaagcc tggcaaggga
     1561 gctcaactgg ccagaagcgc tggatctggc gtacaatttt tagccaaaga gggtgactac
     1621 ggtctggtga agcttccctc tggagaggta cgtcggatca gtcttgattg tatggcggtt
     1681 attggccagg tagggaatct ggactacgag aaagtctcgt tagggaaggc tggtcggtca
     1741 cggtggctag gagtcaggcc ggcagttcgt ggagtggcca tgaatcctca tgaccacccg
     1801 atgggcgggg gcgaggggaa atcctccggc ggtcgccatc catgtagtcc atggggtaaa
     1861 ctagagcgaa aaaccagaaa gaagagaaag gcatcggatc gcttcattat gaagcgcaga
     1921 aaataaggtt tgcaatctac gattcgagtc cttggttgga aatacatgga gactggagct
     1981 cgaaatctga aaatcgaaac tattcacgga ggttagcgtg gcacggtcac taaaaaaggg
     2041 tccgtacgtg gatcctaagc tactgaagaa gatcgaggtg atgaacgatg ggagggagaa
     2101 gaagatcgtc aaatcctggt cgaggcggtc cgttatcctt cccgagttcg ttggccacac
     2161 gctggcgatt cacaacggca agaaatttat cccgatttac attagcgaaa acatggttgg
     2221 gcacaagttg ggggagtttt ctccaactag aaccttccgt ggacacgggt cgcatacggc
     2281 gcgttcttct gcactaaaat aagggggtgg catcgagcat ggaatgtcgc gcagttggcc
     2341 gtttcatcgg aattccggca cggaaggcga ggctcgtcgt tgacctgatt cgcggttacg
     2401 gcattagtca ggccatcgac accatgaagt ttacgaaaaa gtatgccgcc aaggttgtgg
     2461 agaagattct caggtcggcg ctggctaatg cgcaacataa ccatggagca aagaacgttg
     2521 atcgactgtt cgtgaaggag gcctttgtga atcaggggcc gaccacgaag cggtttcggc
     2581 ctcgctccat ggggcgggca aatactataa gaaaacgaag tagccacatc actatcgttc
     2641 tcacggagag ggaatctgcc cgatgaatcc atccttgcga aaggatagga tgggccgctc
     2701 cggtgaggct tagttcgagc gagatggcgg aggcgtaatg ggacagaagg tacaccctat
     2761 aggatttcga ctgggcttca tcaagccatg gaggtccagg tggttcgcca cgaaggggta
     2821 tgcagatgct ctgcatgaag acctcaaatt ccggagcttc atcaaggagc gcgtctatca
     2881 tgctggagtg tccgggatcg atattgagcg aaaggcagat cagattcgta ttataataca
     2941 taccgcaagg cccggtatta ttatcgggaa gaagggttct gaggtcgaca agctgaaagt
     3001 ggccctctcc ggtatgtcta aaaagccgat tcagttggat atcgtcgagg ttcgcagggc
     3061 ggagttggac gctcagttga tcgccgagca ggtagcttct caactgttgc gccgggtggc
     3121 ctataggcga gccatgaaaa agacggtgca atcctcgatg cggcttgggg ctcagggagt
     3181 tcagattgta tgcgccgggc ggctggctgg ggcagagatt gcccgcaagg aacgataccg
     3241 cgaggggcgg atgccattga gtaccatgcg agccgacatc gacttcgggt tcactcaggc
     3301 caagaccacg tatgggctga ttggggtcaa gtgctgggtg tatcatggag aggcgactcc
     3361 tgagaccgtg gcggctcaac cggaacgaga tgcgcctcaa gatcgacagc ggcggcctaa
     3421 ggggcctgcc ctggtgcaga gctagaacgc gatgctgata cctaagcgag ttaaattccg
     3481 gaaacagcac cgtggtcggc gatcaggact cgccattcgg ggatcgacct tggcgtttgg
     3541 cgcatacggt ctgaaggctt tggaagcggc ctggattacc aaccgtcaga ttgaggccgc
     3601 ccgtcgagcg atcacccatc atgtcaaacg gggaggcaag gtgtggattc gaatcttccc
     3661 ggataaaccg ataacgaaaa agccggcgga gacccggatg ggtaagggaa agggtgcgcc
     3721 ggaaggttgg gtggcggtag tcaagcctgg ccgtgtcctc tgcgagatgg agggggttac
     3781 tgaggcaacg gccaaggagg caatgcgcct tgctgcgcat aaactcccca tagccacacg
     3841 gtttgtgtcc cgtacgatgg tggcgagctg aggtgacgat ggttggcatg gatgcgaaag
     3901 cgtttcgtga actgagcgcg gaagagttac aacagaagat ccgcgacgtg agagatgaac
     3961 tcttcaagtt gaagctgagg gcgtcagcgg cgcagcttga gaacccggcg cgtatccggc
     4021 gacttcgccg ggagatcgcc atcggagaga cggtgcggac agagtccttg cgtaaacagg
     4081 cctcaccagg ggtggagcga acgccatgac cgagcaggag aagaagggtc gtcggaagac
     4141 cttagtgggg gtcgtggtga gcgacaagat gcaaaagacc gtcgtcgtga tggtcgagcg
     4201 gattgttcgt catgacgcct atagtaaaat ggtccgccgc cggacaaagg tcaaggccca
     4261 cgac
//
LOCUS       DATMXI010000390         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_27759, whole genome shotgun sequence.
ACCESSION   DATMXI010000390 DATMXI010000000
VERSION     DATMXI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27759"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VN444_07560"
     CDS             complement(<1..776)
                     /locus_tag="VN444_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HWR21701.1"
                     /translation="MTGERFPFPGIPAIADGSAAVVWVDSHITQGACAYPITPSTNMG
                     SGYQAEVANGTRNLWGEPLFFLEPESEHSSASSCEGFALAGGRVSNFTSGQGLVLMKE
                     VLYTISGKRLPAVFHIGARALTSQALNVHAGHDDVMAVADVGWGILFAKNAQDVADLA
                     LIARRAAEEGETPFMTVQDGFLTTHTIENLLLPEPELMKEFIGDPHAGARLRNLMNPS
                     EPIMIGVVQNQDSYMKGKIAQRFFYDRLPAIVERAMEQYYS"
     gene            complement(773..1189)
                     /locus_tag="VN444_07565"
     CDS             complement(773..1189)
                     /locus_tag="VN444_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR21702.1"
                     /translation="MTQLSEIMNRTLVTVDTHASLRQAQRILDRRNIRHLFVMDGKRL
                     VGIVTDRDLRKAAPSSKSPLTASEREEFMDELKVVEIMLRKLITASPSTTVREAAQVM
                     IREKIGCLPVVEGKTLVGIVTETDLLEILVRGEGTS"
     gene            complement(1373..2482)
                     /locus_tag="VN444_07570"
     CDS             complement(1373..2482)
                     /locus_tag="VN444_07570"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWR21703.1"
                     /translation="MTAVRRAVNIGILAMASLLLVRSVALAHGTGVRGTQTLYVASEG
                     SDTVSVMDVGSMKVTGTIAVGAGPQHVAISPDRRYAFVACSRSAEVWILDLPAHAVAA
                     TVPNATGPGGQTVFGAGATYAYVTNSLYSRVTVIETTTGKKVAMIPVGEAPTTISISP
                     DGGHAYVPSERSNTVAALNLSLNVVAAELPVGVRPYAVEISPDGKYLLVSSADSNTVT
                     VMDATARKPLRRIPVGDDPHHMVFGPGGAFAYVLNRGSDNLSVIQMANHQVVATIPVG
                     KEPSDADITPSGHYIYVTNMGSGNVSVISTQTSTVVGTIPVGTHPHNIVISGDGRYAF
                     VANTGSDDISVINLLSQATVGRVPVGKRPLGMALR"
     gene            complement(2479..>3305)
                     /locus_tag="VN444_07575"
     CDS             complement(2479..>3305)
                     /locus_tag="VN444_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HWR21704.1"
                     /translation="PRVNLAAIAAKTPGFVGADLANLVNEAALLAARKGRDAVEMADF
                     DEAIDRIVGGLEKKTRVMNPAEKETVAYHEAGHALVAESRPRADRVSKISIIPRGVAA
                     LGYTQQLPTEDRYLLKRADLLDRLDVLLGGRVAEELVFGDVSTGAQDDLQRATDMARL
                     MVTQYGMSEHLGLATFEEPRTSAFLNIQKPQGVREYSEQTAQAIDEEIRKLLADAHTR
                     VEQTLAARRGELDALGKLLLEKEVVDREALTQLLAPQETEQRAQEWRPVGEKGGVT"
BASE COUNT          631 a         1070 c          950 g          654 t
ORIGIN      
        1 gagtaatact gctccatcgc ccgctcgaca atcgccggaa gacgatcgta gaagaaccgc
       61 tgggcaatct ttcccttcat gtagctgtcc tgattctgta cgaccccgat catgatcggc
      121 tccgacgggt tcatgaggtt acgcaggcgc gcgccggcat gcgggtcccc gatgaactcc
      181 ttcatcagct ccggttccgg aagcaggaga ttctcaatcg tgtgagtggt gaggaagccg
      241 tcctgcacag tcatgaacgg ggtctccccc tcctcggcgg cgcggcgggc gatgagcgcc
      301 agatcggcca catcctgagc gttcttcgca aagaggatgc cccagcccac atccgccacg
      361 gccatcacgt catcgtgacc cgcgtggacg tttaaggcct gagaggtcag cgcgcgggcg
      421 ccgatatgga acaccgccgg cagccgtttc ccggaaatgg tgtagaggac ctccttcatc
      481 aggaccagcc cctgaccgga ggtaaagttc gagactcgcc cgccggccag agcaaaaccc
      541 tcacacgagg aagcggagct atgctctgac tctggctcaa ggaagaagag cggctcgccc
      601 cagaggttgc gtgtaccgtt cgcgacctcc gcctggtacc cgctccccat gttggtggat
      661 ggggtgatcg gataggcgca ggcgccctgc gtaatatgcg agtctaccca gactactgcc
      721 gccgagccgt ccgctatcgc cgggatgccg ggaaagggaa atcgttcgcc ggtcatgacg
      781 tcccctctcc ccggaccagt atttcgagga gatcggtctc ggtcacgatt ccaaccagcg
      841 tcttgccctc caccaccggc aggcagccga tcttttcccg gatcatcacc tgcgctgcct
      901 cccgcacagt ggttgaagga gaggccgtga tcaacttgcg caacatgatc tccacgacct
      961 tcaattcgtc catgaactcc tcgcgctcac tggccgtcaa aggcgacttg gaggagggag
     1021 ccgcctttcg aagatcgcga tcggtgacga tcccaaccaa tcgcttgccg tccatcacaa
     1081 acagatggcg gatgttacgc cgatcgagga ttcgctgagc ctggcgcaga gaggcgtgcg
     1141 tgtccacggt gaccagcgtc cgattcatga tctctgaaag ctgcgtcatc tgtttctcct
     1201 cgtaactact taggccttta gactgaaggc tgaaggctat aactgatctc gcaagcgcga
     1261 atcaagccat tcaagacctt ctcggtttct acgaccttgt tgtgtgatcg ctcatgattt
     1321 acccctaaaa gccttcagcc ttcagcctat ccacctgaat agtctctgct cctcaccgta
     1381 aggccatccc aagggggcgc ttgccgacag gcactcgccc taccgttgcc tgggatagca
     1441 ggttgatcac ggagatatca tccgatccgg tgttagccac aaaggcgtac cgcccgtcgc
     1501 ccgagataac gatgttgtgg ggatgggtcc ctacagggat cgtccctacc acggtgctgg
     1561 tttgcgttga gatgaccgac acgttgccac tgcccatgtt ggtcacatag atgtagtggc
     1621 cggatggggt gatgtcggcg tcgcttggtt ccttcccaac ggggattgtc gctaccacct
     1681 gatgattcgc catctggatg acgctcaggt tgtccgagcc cctattgaga acgtacgcga
     1741 aggcgccacc aggaccgaac accatgtgat gcgggtcgtc accgacgggg attctgcgca
     1801 acggcttccg ggctgtggcg tccatgacgg tgacggtgtt tgaatcagcg ctggagacca
     1861 gcagatactt gccatcagga gagatctcga cggcataggg cctcacgccg accggtagct
     1921 ccgccgcaac gacattcagc gaaaggttca acgcggcgac ggtgttggat cgctcgcttg
     1981 gaacataggc gtggccacca tccggcgaga tgctgatcgt ggtcggcgct tctcccaccg
     2041 ggatcatcgc caccttcttt ccggtggtag tctcaatcac cgtgactcgg ctgtacagac
     2101 tgttggtcac gtaggcatag gtcgcgccgg ctccaaacac ggtctggccc cccgggccgg
     2161 tagcattggg caccgtagcg gcgacagcgt gcgcgggcaa gtccagaatc cagacctcag
     2221 ccgaacggct acacgcgaca aaggcataac gccgatccgg gctgattgcg acatgctgcg
     2281 ggccagcgcc cactgcaata gtgccggtca ccttcattga cccgacatcc atcaccgaaa
     2341 ccgtatccga gccctcactc gcgacataga gcgtctgcgt accgcgcaca cctgtcccgt
     2401 gcgcgagcgc aaccgagcga acgagcagca gcgacgccat tgccaggatg ccaatgttca
     2461 cggcacgccg gactgccgtc atgtcacccc tcccttctct ccgaccggtc tccattcttg
     2521 agccctctgc tccgtttcct ggggcgccag caactgcgtc agagcctccc gatcgaccac
     2581 ctccttttcc agcagcagct tgcccagcgc gtccaattct ccgcgtctgg cggcgagcgt
     2641 ctgctcgact cgggtgtggg catcggccag gagcttccgg atctcctcat caattgcctg
     2701 agcggtctgc tcgctatact cccgtacccc ctgaggcttc tggatgttga ggaaggcgga
     2761 ggtgcgcggc tcttcgaacg tcgccaagcc gagatgttcg ctcatcccgt actgggtcac
     2821 catgaggcgg gccatatcgg tggcccgctg aagatcatcc tgggccccgg tggagacgtc
     2881 cccgaagacg agttcttcgg ccactcgacc ccccaggagc acgtcaagtc gatcaaggag
     2941 gtcagccctt ttgagcaggt accgatcctc cgtgggcaac tgctgcgtgt agccgagcgc
     3001 ggccacgccg cgagggataa tcgagatctt cgagacgcga tcagcgcgag gacgagattc
     3061 cgctaccagg gcatggccgg cctcgtggta ggcgacagtt tccttctccg cgggattcat
     3121 cacccgggtc tttttctcga gaccgcccac gatccggtcg atcgcctcat cgaaatccgc
     3181 catctccacc gcatccctgc ccttgcgcgc agccagcagc gccgcctcgt tcaccagatt
     3241 ggccagatcg gcgccgacga accccggagt cttcgcagcg atggcagcga ggttcactct
     3301 cggcg
//
LOCUS       DATMXI010000391         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_270791, whole genome shotgun sequence.
ACCESSION   DATMXI010000391 DATMXI010000000
VERSION     DATMXI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270791"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VN444_07580"
     CDS             complement(<1..503)
                     /locus_tag="VN444_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWR21705.1"
                     /translation="MRCDEVECKLIELIEEDLPPTQRAEILAHLHGCATCAAARSAYH
                     DLLALVQADPVPEPSPGFWEEFLPSLKHRIEQEACRHRPTPTAWLAGAGSWFTFRPRL
                     IAGLAVAAVSMFIVVRLPGFLPIRADRQMTPISTEKAASQNGGGYNVAMAPRADDGNQ
                     RPGEPLVV"
     gene            complement(527..1078)
                     /locus_tag="VN444_07585"
     CDS             complement(527..1078)
                     /locus_tag="VN444_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWR21706.1"
                     /translation="MREVDFELIDRFLRGDGTAFDELVQRRQREVYNLAYRMTRNAED
                     ARDVSQEAFLQVYRNLSRFDRRSSLSTWLYRIVVNLCLNHLNRGSRSLYATLDQHPDP
                     VDSSEGSLARLEENERADALARAIETLPTQQRASLTLRVHHHLAHREIAEILKVSEAT
                     AKVHYFHAVQALRRKLAHWREGA"
     gene            complement(1209..1304)
                     /locus_tag="VN444_07590"
     CDS             complement(1209..1304)
                     /locus_tag="VN444_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HWR21707.1"
                     /translation="MGSVVKKRRQKMSKHKHKKLLKRTRHQRRKK"
     gene            complement(1305..>2149)
                     /locus_tag="VN444_07595"
     CDS             complement(1305..>2149)
                     /locus_tag="VN444_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWR21708.1"
                     /translation="LLESEIFGHERGAFTGAVERRAGCFELADGGTIFLDEVAEMQLP
                     TQAKFLRVLQGETFRRLGGKNEIDVDVRVIAATNRDPVEAVKDGLLREDLFYRLNIVS
                     IVIPPLRERSTDIPQLVEYFIKKHGESAGKPIQGISNEAMRLLMNYHWPGNVRQLEAV
                     VERASLLSEGPEIKHYDLPIEVRFFNLPVQLAPSSSSGRKFAIEIPEQGIHLEALERD
                     LILQAMERSDWVITKAARLLGLSYRTLQYRLEKFQIKRDTKTALVTPESELTEHTQEQ
                     KGGM"
BASE COUNT          426 a          685 c          584 g          454 t
ORIGIN      
        1 accacgagcg gctcaccagg gcgttgattc ccgtcatcgg cccgtggcgc catcgccacg
       61 ttgtaacccc cgccattctg actcgcggct ttttccgtag aaataggtgt catctggcga
      121 tccgccctta tgggcaagaa gcctggcaac cggacgacga tgaacattga aacggccgcc
      181 accgccaagc cggcgatgag gcggggccgg aaagtaaacc acgaccctgc gccagccaac
      241 caggcggtgg gcgtcggcct gtgcctacag gcttcctgct cgattcgatg cttcagcgaa
      301 ggcaggaact cttcccaaaa gcccggcgac ggctcaggaa cagggtcggc ctgcacaagc
      361 gccaagagat cgtgatacgc ggagcgcgca gcggcgcacg tcgcacagcc atgaagatgc
      421 gcgaggatct cagcccgctg cgtgggcggc agatcctcct cgatcaactc gatcagctta
      481 cactcaactt catcacatcg catggcgacg ttgatccttt cgcccctcag gcgccttcgc
      541 gccagtgcgc cagcttgcgc cgtagcgctt gcactgcatg aaaatagtgt accttggccg
      601 tagcctcgga gaccttcagg atttcagcga tctcccggtg tgcgaggtga tgatggacgc
      661 gcaacgtcag gctcgcccgt tgttgcgtgg gaagggtttc gatggcgcgg gcgagcgcat
      721 ccgcccgttc attctcctcc agccgcgcaa gcgacccttc cgatgaatcg accggatcag
      781 gatgctgatc aagcgtggca tagagggaac gcgagccccg gttcagatgg ttgaggcaga
      841 ggttcacgac gatccggtac agccacgtag agaggctgga tcgccgatca aatcggctta
      901 agttccgata cacctgtaag aaggcctctt gcgacacgtc ccgcgcatcc tccgcattcc
      961 tggtcatccg atacgccaga ttgtacacct cccgctggcg cctctggacc agttcatcga
     1021 acgccgtccc atcgccacgc aagaatcgat cgatcagttc aaaatctacc tcgcgcaaag
     1081 cctttgtctc tcaccgagag ctatcccccg tccgaccggc ccggcccagc aacagtactc
     1141 ctacagggca cgtcgcctgg atccgcatat tagaacgtct cagccggccg aacgtttaat
     1201 gcccggattt acttctttcg gcgttggtgc ctggttcgct tcagaagttt cttatgtttg
     1261 tgcttactca tcttttgacg acgcttcttg acgacgcttc ccactcacat cccccctttc
     1321 tgttcttgcg tgtgctcagt taattcgctc tccggagtta cgagagcggt cttcgtatcc
     1381 cgtttgatct gaaatttctc tagacgatac tgcagtgtcc ggtagctcaa gcccaggagg
     1441 cgcgccgcct tggtgatcac ccaatcggac ctctccatgg cctgcaggat caagtcacgc
     1501 tccagtgcct ccaagtgaat tccctgttcg ggaatctcga tcgcgaactt gcggccagag
     1561 gagctcgatg gggcaagctg gaccgggagg ttaaagaacc gaacctcaat aggaagatcg
     1621 taatgcttga tctcaggtcc ttcggagagc aagcttgccc gctcgaccac cgcctccagt
     1681 tgccggacat taccaggcca gtggtagttc atgaggagcc gcatcgcctc gttactgatc
     1741 ccttggatgg gcttccccgc gctctcaccg tgctttttga tgaaatactc gacgagctga
     1801 gggatgtcgg tgcttcgctc gcggaggggt gggatcacaa tggagacaat gttcagccga
     1861 tagaacaggt cctcccggag gagcccgtct ttgaccgcct cgacaggatc ccgattggtt
     1921 gcggcgatga cgcggacgtc aacgtcgatc tcgtttttcc ctcccagcct tctgaacgtc
     1981 tctccctgca ggacacgcag gaatttagcc tgagtcggca gttgcatctc ggcgacctca
     2041 tcgaggaaga tcgtcccgcc atccgctagc tcaaaacatc cggccctccg ctccaccgcc
     2101 cccgtaaacg cgcctcgctc atggccgaag atctcgcttt ccaacagac
//
LOCUS       DATMXI010000392         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_251829, whole genome shotgun sequence.
ACCESSION   DATMXI010000392 DATMXI010000000
VERSION     DATMXI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251829"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1265)
                     /locus_tag="VN444_07600"
     CDS             complement(114..1265)
                     /locus_tag="VN444_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HWR21709.1"
                     /translation="MTRKPGASSGGPAAIRMGIKGLHPAYFGLVMATGIVSIAAGLQG
                     MEVIAEALLWLNVIAFAVLWLLTGLRIVRFPRRFAADLLNHSRGPGFFTIVAACGVLG
                     TQLFLAGKSQQAAVALWFLAVALYAGLTYGMFTGFVTKRAKPSLSEGINGGWLLSVVA
                     TQAIAVLGGLIAQQSELYREVTLFFALVMWLFGGMLYIWIISLVFYRYLFFPFSPADL
                     TPPYWINMGAMAISTLAGTVLIGKAPDFDLLQRMLPFLYGMTLLFWATATWWIPMLVT
                     VGVWRHVIRRFPLAYDPLYWGAVFPLGMYTVCTFRLAEVTELPILMMIPRYFVYVAFG
                     AWVAVFIGLMHQLIGSLMCAARTPMSVRVEEQTPDRNTSLPGVRKHDGR"
     gene            complement(1336..2019)
                     /gene="narI"
                     /locus_tag="VN444_07605"
     CDS             complement(1336..2019)
                     /gene="narI"
                     /locus_tag="VN444_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HWR21710.1"
                     /translation="MNSLNFVLFAAFPYLALFNAVVGTVWRFNRDRFSFSSISSQFLE
                     NRRLFWGSVPWHFGIIIILLAHLIAFLFPGPWRRLLSNIHILTTLEVIGLALAVFAIV
                     GILTLTWRRLTNPRVRATSSVMDWVLLSLLITQVILGFWVALNYRWGADWYMDTSVPW
                     IGSLFTFQPKIEYVVDLPFIAKLHMLLGFGIIGLFPFTRLVHIISFPLRYLWRPLQVV
                     IWNRHMGKV"
     gene            complement(2029..2919)
                     /locus_tag="VN444_07610"
     CDS             complement(2029..2919)
                     /locus_tag="VN444_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HWR21711.1"
                     /translation="MRSIGSTVVCHALDRLGRLLQYPDEHLPVYVREARQQIRSMIPA
                     ADDELAFFETFVNTHDLAYWRQIYTDAFDLDPLFKIYIGYHLFGETYKRSHFLVKLNE
                     HYCAHEYDCRPELPDHLAVVLRFLPHCDHEEFREGLIVEGIVPSIKNMLGKSAATDCG
                     EDLAAQHTTVRSLQDAIRAEQSQFYQPDEIAASWHPEAFGQGVITEGLKDAPHADHLP
                     FGAPSMGGHEFDRSSCGPCPLATGPLTGDLGTQLKGVPEHEKEKDMRKEEVRKGEEER
                     LSHPYAHVLRALEQVLEGKA"
     gene            complement(2922..3308)
                     /locus_tag="VN444_07615"
     CDS             complement(2922..3308)
                     /locus_tag="VN444_07615"
                     /inference="COORDINATES: protein motif:HMM:NF026061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21712.1"
                     /translation="MAPQFNLILEKARVPIKYLASLFTAGNERIIEDVMKRLITVRMF
                     KRWQTVVDLKEADVARALADANMTPEEAEAIFRLTSLPTYEERFVVPPLKREQSIEST
                     RDPLTQKREAGFTRFPGSQKEPARGW"
     gene            complement(3287..>3619)
                     /locus_tag="VN444_07620"
     CDS             complement(3287..>3619)
                     /locus_tag="VN444_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21713.1"
                     /translation="DQIRSAAGVPDDRLVDAQREMVLNPHDEQVVTAARRNGIGDDMI
                     EAARNSPVYKFFKQWRIALPLHPEFRTLPMLFTCHRFSRFRRRSGKRPGPTRSMELAR
                     RAWRPSST"
BASE COUNT          789 a         1075 c         1057 g          698 t
ORIGIN      
        1 gcttcaggcc tgcgacggga atggcggtga gttcgccgca cttgatgtcg ctggcgacgg
       61 cgagcttcga gatgatggct acttatcccg cccgccgctt ggatacgggc gcctcagcgt
      121 ccatcgtgct ttctgactcc aggaagagat gtgttgcgat ccggggtctg ttcctctacc
      181 cgtacgctca tcggcgtgcg ggctgcgcac atgaggctgc cgatgagctg gtgcatgagc
      241 ccgatgaaga cggccaccca tgcgccgaag gccacataga cgaagtagcg ggggatcatc
      301 atgaggatcg gcagttccgt tacctctgcc aatcggaatg tgcagaccgt gtacatgccc
      361 agcgggaaga cggcccccca gtacagagga tcgtacgcca gcgggaacct ccgtatgacg
      421 tgtctccaca ccccaaccgt gacgagcatc gggatccacc acgtcgcagt cgcccaaaag
      481 agcagggtca tcccgtagag aaacggcagc atgcgttgca acagatcgaa atccggggct
      541 ttaccgatca gcacggtacc ggccagcgta gagatggcca tcgcccccat gttgatccag
      601 taggggggtg tgaggtcggc cggtgagaac ggaaagaaaa ggtaacggta aaagaccaga
      661 gagatgatcc agatatagag catgccgccg aatagccaca tgaccagcgc gaagaacagg
      721 gtcacctcac ggtatagttc cgattgctgg gcgatgagcc cgccgagaac ggcaatcgcc
      781 tgcgttgcga caacactgag cagccatcct ccattgatac cctcgctgag agaaggtttc
      841 gcccgctttg tcacaaagcc ggtaaacatc ccgtaggtca aacccgcata cagcgccaca
      901 gccagaaacc atagcgccac cgctgcctgc tgacttttgc ctgccaggaa cagttgagtc
      961 ccgagcacgc cgcacgcggc gacgatggtg aaaaagcccg ggccgcgact gtgattcaag
     1021 agatctgcgg cgaaacgccg cgggaatcgg acaatccgca gacccgtcaa cagccagagc
     1081 accgcgaagg cgatcacgtt gagccatagc agcgcttcgg cgatcacctc catcccctgg
     1141 aggccggccg cgatcgagac gatgccggtc gccatgacga gtccgaagta ggcggggtgc
     1201 aggcccttga tacccatccg gatcgctgcc ggccctcccg acgatgctcc aggcttgcgc
     1261 gtcacctatt cctcgggcgt tcgtcatagc cccgtggcca tccatgtatc atgggctcat
     1321 caaaatcctg actccctata ccttccccat atgccggttc catatgacga cctgcagggg
     1381 gcgccagaga taccgaagtg gaaacgagat gatgtgtacc agcctggtga acggaaacag
     1441 accgatgatg ccgaacccta acagcatatg gagtttcgcg atgaagggga ggtctacgac
     1501 atattcgatt ttgggctgga atgtgaagag cgagccgatc cacgggaccg acgtgtccat
     1561 gtaccagtcc gcgccccagc ggtagttcag cgcgacccag aatccgagga tgacttgcgt
     1621 gatgagcaga gacaataaca cccaatccat cacgctggag gtcgcgcgga cccgcgggtt
     1681 cgtcaaacgc cgccaggtaa gggtgagaat tcctacgatg gcaaacaccg cgagggctag
     1741 gccgatcact tccaatgtcg tgaggatatg gatattggac agcaatcgcc tccagggtcc
     1801 ggggaacaga aaggcaatca ggtgggccag taagatgata atgatcccga agtgccaggg
     1861 gacggaaccc cagaacagcc tccggttctc caggaactgg gatgagatgc tggaaaagga
     1921 aaaccggtcg cgattgaacc gccagacagt ccccactacc gcgttaaaga gtgccaggta
     1981 aggaaacgcc gcaaacagca cgaagttcag cgagttcatg gaaaccctct acgcctttcc
     2041 ctctaatacc tgttccaagg cgcgcaggac atgcgcatac gggtgcgaca gccgctcctc
     2101 ctcacccttt cgcacctctt ctttccgcat atctttttcc ttctcatgct cgggaacgcc
     2161 cttgagctgt gtcccgaggt ctcccgtcaa cggtcccgtc gcgagcgggc acggaccaca
     2221 ggacgaccga tcaaattcat gacctcccat cgatggggcg ccgaagggta ggtggtcggc
     2281 gtgtggcgcg tctttcaatc cttcggtgat gacgccctgt ccgaacgctt ccggatgcca
     2341 cgacgcggca atctcatcag gttgataaaa ttgactctgc tcggcgcgaa tggcgtcctg
     2401 aagagatcgt actgtcgtgt gttgcgccgc gagatcctct ccacaatcgg tcgcggcgct
     2461 tttgccgagc atgttcttga tcgatggaac gatcccctcc acaatcagcc cttcccggaa
     2521 ctcctcatgg tcgcaatgcg gcaggaaccg aaggaccacc gccagatgat caggcagttc
     2581 cggtctgcaa tcgtactcgt gtgcgcagta atgttcgttc agcttgacca gaaaatgact
     2641 gcgcttatac gtctcgccga acaggtggta gccgatgtag atcttgaaca gggggtcgag
     2701 gtcgaaggcg tcggtgtaga tctgcctcca gtatgccagg tcgtgggtgt tcacgaacgt
     2761 ttcgaagaaa gccagttcgt catcagccgc agggatcatg gatcggatct gttgacgggc
     2821 ctcccgcacg tacacgggca gatgctcgtc ggggtactgc agcagtcgtc cgagtcgatc
     2881 caatgcgtgg cacacgacgg ttgacccgat ggagcgcatg actaccatcc ccgtgccggt
     2941 tctttctgac tgccgggaaa gcgtgtgaat ccagcctcgc gtttctgtgt gagaggatcc
     3001 cgggtcgatt cgatagattg ctcacgtttg agcggcggca caacgaatcg ttcctcatac
     3061 gtggggagcg aggtcagtcg aaaaatggcc tcggcctcct ccggtgtcat attggcatcg
     3121 gccaaggctc gcgcgacatc cgcctcttta agatcgacca cggtttgcca gcgcttaaac
     3181 atccgcaccg taatcagccg tttcatcacg tcttcgataa tcctttcgtt gcccgccgta
     3241 aacaggcttg ccaggtactt aatggggacg cgggccttct ccaggatcag gttgaactgg
     3301 ggcgccatgc cctccgcgcc agttccatcg atctcgtagg ccccggtcgc ttcccggatc
     3361 gtcgcctgaa gcggctgaag cggtggcacg taaacagcat cggcagcgtg cgaaactccg
     3421 gatggagggg tagcgcgatc ctccattgct taaagaattt gtagaccggg gagttcctgg
     3481 cggcctcgat catatcgtcg ccgatgccgt tgcgcctggc cgccgtcacg acctgttcgt
     3541 cgtgcggatt gagcaccatc tctcgctgag cgtcaaccag gcgatcatcg ggaacgccgg
     3601 ccgccgatcg tatctgatc
//
LOCUS       DATMXI010000393         3395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_171882, whole genome shotgun sequence.
ACCESSION   DATMXI010000393 DATMXI010000000
VERSION     DATMXI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171882"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN444_07625"
     CDS             complement(<1..282)
                     /locus_tag="VN444_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21714.1"
                     /translation="MSDPVVQGRACPALTQAPQYRPSVGALDQLDGIHRQSSPPSDLF
                     HYDCGLLYEINGRLDDDEGMYRKAITLKTNDVFLKAIRAVRLTRKDHRCV"
     gene            1096..1299
                     /locus_tag="VN444_07630"
     CDS             1096..1299
                     /locus_tag="VN444_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21715.1"
                     /translation="MVITLKVDTSSYDRLVARGQVKRGDLLGFSPYFDGQVILSPIDG
                     TIEHITYNREERALIVSICTQGV"
     gene            1337..1696
                     /locus_tag="VN444_07635"
     CDS             1337..1696
                     /locus_tag="VN444_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWR21716.1"
                     /translation="MRYRIRKFKGSLIIELDGKAQPDESARARWVLSPFLRHPGVKAI
                     LYLAGLRKIGIRELEVLEVIRKEVKTQRGSLRLCALRDDLRAKFDRNPFLRVYELYDD
                     LESSLSEMPDLPLKRSA"
     gene            1859..3214
                     /locus_tag="VN444_07640"
     CDS             1859..3214
                     /locus_tag="VN444_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWR21717.1"
                     /translation="MRRFLHRFLVAGTVVMIALSFAVPSQTAWSAEKKDDKAVLDEVL
                     DILKEKGQLTEEKYQELKTRAKKEGTGKILAGFEGWTPTFKSADGQHKIELHGRLNFD
                     LRAYEDDARALDSKRSNQDPNRSDLLTRMLVRRARLGVDATFFKYLDMKVEGEFSEVG
                     SASNADSKFQLTDGYAELRYWPEFRVRGGQFKVPFSFEELTSSRFIDFVERSAINNLV
                     PARDVGAMAHGSLLGGTLEYSAGIFNGTGQNNPELNDSKDYVGRLLVRPFKLTDIPLL
                     QNLHLAGHMTYGDQDKGKSLRGRTDGRFEFFPRVDTSGDRVRYGFEAVNAYGPFGLYG
                     EYVKTAEERKGLGSGGSDLADVDGRGWYVAGTWLVTGEEKIHGKAPKVKKNFDPRTGG
                     LGALELVARFAQLDFDSDDSLSSPGENGVDTLTVGFNWYLSPNARLMFNWGQNGLTTT
                     R"
BASE COUNT          765 a          948 c         1005 g          677 t
ORIGIN      
        1 cacgcaccga tgatccttcc tggttaggcg gacagctctg atggccttaa ggaagacgtc
       61 gttcgtcttg agcgtaatgg ccttccgata catcccctcg tcgtcatcga ggcgaccgtt
      121 gatctcatag aggagcccac agtcatagtg gaagagatct gatggtggcg atgattgtcg
      181 gtgtatcccg tcgagttggt ctagcgcccc aacggaaggc cggtattgag gtgcttgtgt
      241 cagcgccgga cacgccctac cctgtaccac agggtcactc aaaaccctcc tgtcagatat
      301 gtttgtgccc gaggctacgg gtggattacg tcatctcgaa aacgcgccta tagtgttccc
      361 cccccctgtc tgtcaagcag aaaatgctga ggctcagcga atatggatga ataagcgaga
      421 tacgaaggga aatacattcc atattgaccc cagtacagca gaagtgctcc gaccatgaat
      481 actttgatag gctccaggca agcgtgactg gcggggtacg tgaatgctct gcgtttgcaa
      541 agctggtgag attgcgtcgg cggcgcgctc tcctgtctgc gtacggatac acacaggcgg
      601 atgagcataa acgtttgtgg cgagaccgta ccactcactg gctcgtgatt ggttagaaag
      661 tcccccgccc tcagcgtagg ggcgaccctc gtggtcgccc gatccgggca ggcacaaggc
      721 ctgcccctac aatggggagg gtgagggaga agatgaggcc tttcatgccc atgggcgtgc
      781 caccggcgca tggggtattg cgagcgaccg acgggagcgc ggcgatccca ccgcacactt
      841 acagtgcgag attgcttcgg tcgctacgct ccctcgcaat gacccttgtg gggaactttc
      901 tcggcaatga cagctacggc gtttagccga gttgtcgggt tacgctacgc taacccgacc
      961 tacgaccctt cgatctaatc cccatcatca aggatctccg ccaatcgctg ggcctcaaat
     1021 ttactctggt gtcatcgatc cttcatatga tcgtaataaa ggacggttag gataccagtc
     1081 aaaaaggagg atcgaatggt gattacgttg aaggttgata ccagttccta cgatcggctt
     1141 gtcgcaagag gccaggtaaa gaggggtgac ctgttgggct tctctccata ctttgatggt
     1201 caggtcatct tgagtcccat cgatgggact attgagcaca tcacgtataa cagagaagaa
     1261 cgggcgctga tcgtcagcat ctgcacacag ggcgtatagg cgcaggcggc tcaccaataa
     1321 cgaggaggag cggagcatgc gataccggat caggaaattc aaggggagcc tgattatcga
     1381 actggatggc aaggcgcagc ccgacgagtc agccagagca cgctgggtcc tctcgccgtt
     1441 tctccggcac cccggcgtga aagccatcct ctacctggcc gggctgcgca agatcggtat
     1501 ccgagagttg gaggtcttgg aggtaatccg taaggaggtt aagacgcagc gcggctctct
     1561 ccggctgtgc gcccttcgag acgaccttcg ggcaaagttc gatcggaacc ccttcctacg
     1621 agtctacgaa ttgtatgacg atctggaaag cagcctctca gagatgccgg acctgcccct
     1681 gaagcggtca gcctgaatcg agcgatagga gccggccatg tgtaagtatc tgcgacgtcc
     1741 gccatcaaga gaacaggcgc ctagtgtcaa cactcagtcg gcctcacaac ccaaggtttt
     1801 aagccacaac gccgaagttc ccattaacaa tctctatcac acaaggagag aaaacacgat
     1861 gagacgattt cttcacagat tcttggttgc aggcacagtg gtcatgattg cgctgtcatt
     1921 cgctgtcccc tcgcagacag cctggagcgc cgagaagaag gacgataagg cagtcctgga
     1981 cgaggtgctg gacatcctaa aagagaaggg ccagcttacc gaggaaaagt accaggaact
     2041 caagacgcgg gccaagaaag aaggaacggg caagatcctg gctggattcg agggctggac
     2101 gcccaccttc aagtctgccg acgggcagca caagatcgag cttcacggga ggctcaactt
     2161 cgacctgcgg gcctacgagg atgatgcccg tgccctcgat tcgaaacgga gtaatcaaga
     2221 tccgaaccgg agcgacctgc tcaccaggat gctggtgcga agagccagac ttggagtcga
     2281 tgcgaccttc ttcaaatacc tcgacatgaa ggtagaaggt gaattctccg aggtcgggag
     2341 cgcgtcgaac gccgactcta agttccagtt gaccgacggt tacgctgaat tgaggtactg
     2401 gcccgagttc cgggtccgag gcggccagtt taaggtcccc ttcagcttcg aggagctgac
     2461 ctcttcccgg ttcatcgact tcgtggagcg gtcggccatc aacaacctgg tcccggcccg
     2521 cgatgtcgga gctatggcgc atgggtctct ccttgggggg acgctcgagt attctgccgg
     2581 aatcttcaac ggcaccggac agaacaaccc ggaactgaac gactccaagg actacgtcgg
     2641 ccgtctgctg gtccgcccat tcaagctgac cgacatccca ctactccaaa atctccacct
     2701 ggcaggccac atgacctacg gcgaccagga taaaggcaag agtctccggg ggcgaaccga
     2761 cggcagattc gagttcttcc cgcgagttga caccagcggc gatcgggtcc gctacggctt
     2821 cgaggcagtc aacgcctacg gcccctttgg gctgtacggc gagtacgtga agaccgcgga
     2881 ggagcgcaag gggctcgggt ctggagggag cgaccttgcc gatgtcgacg gccggggttg
     2941 gtacgtcgcc gggacatggc tcgtgacggg tgaggagaag atccacggga aggcacctaa
     3001 agtcaaaaag aacttcgatc cccgaacagg tggcctgggg gcgctggagc ttgtagcccg
     3061 atttgcccag ctcgactttg actctgacga ttccctttcc agccccggtg agaacggcgt
     3121 cgatacgctg accgtcggct tcaactggta tctcagtcca aacgcccgcc tcatgttcaa
     3181 ctggggccag aacggtttga caacgacaag atgaccccga tcaagggtaa ggacacgtcg
     3241 tgggagatca cgtcgcgcct ggccctgtgg ttttaaggta actcgctgta tttgagcaac
     3301 ctgtgtacgt tgccctggac gctcatcgcc ttctctgatc cggggagttc cccctcgctc
     3361 ggacgggacg gcgatgggcc gatctggggc agcaa
//
LOCUS       DATMXI010000394         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_244472, whole genome shotgun sequence.
ACCESSION   DATMXI010000394 DATMXI010000000
VERSION     DATMXI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244472"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..719
                     /locus_tag="VN444_07645"
     CDS             150..719
                     /locus_tag="VN444_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HWR21718.1"
                     /translation="MRQMKVATVFTSLLVVIVGAIALWNNESVTVNRPAVQRAASEPM
                     KSAFESERKTVALFFVASDGNSFHEETREIGGGATTTEDAKRALTELVNGPKGGGLAP
                     TIPRDAKLLNLFIDSSGTAYVDFNQGLRDRLSGGAQGELYTVFSIVNTLASNFVQITR
                     VQILVEGAEISTLAGHVDTRMALLPQYVF"
     gene            755..1495
                     /gene="rph"
                     /locus_tag="VN444_07650"
     CDS             755..1495
                     /gene="rph"
                     /locus_tag="VN444_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWR21719.1"
                     /translation="MRSDGRKIDENRPVKVDRGFLKYAEGSLLIEVGETKVLCTATVE
                     EKVPLFLRGTGQGWVTAEYGMLPRSTKTRIPRESATGRASGRTFEIQRLIGRSLRAVI
                     DLTRLGERTVLVDCDVIQADGGTRTTAITGAFVAMVDALSRLQENGQLKRPLLKDFVA
                     GVSVGYVDGELLLDLNYAEDSMAEVDMNVVMTGSRKFVEIQGTAEEVPFDKAQLDQML
                     HMATQGIDKLVDLQRQLLGAELNGLKTA"
     gene            1532..2134
                     /gene="rdgB"
                     /locus_tag="VN444_07655"
     CDS             1532..2134
                     /gene="rdgB"
                     /locus_tag="VN444_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWR21720.1"
                     /translation="MQLVLATANVGKLQEIIAILSDLRISFLSLASLHGYIPPVESGV
                     TYAENAAAKAKAAAEISGCWALADDSGIEVDALGGQPGVHSGRYLRLTATDQERNQRI
                     LELLDGIPLPQRGARFQCVVAVAGPGGELSIFHGICHGSIAEAPCGNSGFGYDPIFIV
                     SDLGVTMAELPPDVKNRISHRARAIEKTKSLLRRLSSATV"
     gene            2183..2259
                     /locus_tag="VN444_07660"
     tRNA            2183..2259
                     /locus_tag="VN444_07660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2217..2219,aa:Pro,seq:tgg)
     gene            2349..2426
                     /locus_tag="VN444_07665"
     tRNA            2349..2426
                     /locus_tag="VN444_07665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2384..2386,aa:Arg,seq:ccg)
     gene            2479..2553
                     /locus_tag="VN444_07670"
     tRNA            2479..2553
                     /locus_tag="VN444_07670"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:His,seq:gtg)
     gene            2611..2687
                     /locus_tag="VN444_07675"
     tRNA            2611..2687
                     /locus_tag="VN444_07675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Arg,seq:tcg)
BASE COUNT          608 a          686 c          888 g          619 t
ORIGIN      
        1 tgcgaggccg acaaagtcga ccgtagcaat ctcacagtga aaggaagtgt gattttcgcg
       61 tcgcattcgg aaacgcctgg ctcatccgtg cgcgttccta cagtctatct agctaagtag
      121 ttacgggaga gggttgtgga aatatgacca tgcgtcagat gaaggtcgcg accgtcttta
      181 cctctttgct agtggtgatc gtcggtgcga tcgctctttg gaacaacgaa tcggtcacgg
      241 tgaatagacc cgctgtccag cgtgctgcct cggagccgat gaagtcggcg tttgaaagtg
      301 agagaaaaac cgtagcgctc ttctttgtag cgagtgacgg caattcgttc cacgaggaga
      361 ccagagagat tggaggtgga gctacgacga cagaagatgc caagcgagcc ttgactgagc
      421 tcgtaaatgg gccgaagggt ggcggtctag caccgacgat ccctcgggat gccaagctcc
      481 tcaatctgtt tattgattca tccgggactg cgtacgtcga tttcaatcag ggattacggg
      541 atcgtctgtc tggcggcgct caaggggagc tctacaccgt attctctatt gtgaataccc
      601 tcgcctcgaa cttcgtccag atcacgcgcg tgcagatcct cgtggaggga gctgagattt
      661 caacattggc aggacatgtt gacacacgaa tggctctact gccacagtac gtcttttgag
      721 atggttgata ggtgattgcg cgaaaggaga gactgtgagg agtgatggtc ggaagattga
      781 tgagaatagg ccggtaaagg tggaccgcgg ctttctcaag tatgctgagg ggtcactgct
      841 tattgaggtt ggtgagacta aagtcctctg cacggccacg gtagaggaaa aggttccgct
      901 gttcctgcgt ggaaccgggc aggggtgggt cactgcggaa tatgggatgc tcccccgctc
      961 gaccaagacg aggatacctc gggaatcggc gaccgggagg gctagcggcc gaacgttcga
     1021 gattcagcgt ctcatcggcc ggtccctgcg ggcggtgatc gatttgacac gattggggga
     1081 gcggacagtg ctggtcgatt gtgacgtcat tcaagctgac ggagggactc gaaccaccgc
     1141 tatcacaggg gcctttgtag caatggtcga cgcgctctct cgccttcaag agaacggcca
     1201 actcaagaga ccgctcctga aagatttcgt agctggtgtg agcgtcggat atgtggatgg
     1261 agaactcttg ctggatctga actatgccga agactccatg gccgaggtgg atatgaatgt
     1321 ggtgatgaca ggctccagaa agttcgtcga gatccaaggg accgccgagg aggttccctt
     1381 cgataaggcc cagttagacc agatgctcca catggcgacg caaggtatcg acaagctcgt
     1441 tgaccttcaa cgccaacttt tgggggctga gctcaacggc ctgaagacgg cctaaagatg
     1501 tcgcttgaca tcagggggcg aggtttctag aatgcagtta gtgctggcta cggcgaacgt
     1561 aggaaagctt caggaaatca tagccatcct cagcgatctg aggatttctt ttttgtcgct
     1621 cgcctcgctt cacggatata ttccgccggt cgagtctggc gttacgtacg cggagaacgc
     1681 cgctgccaag gcaaaggcag ccgcagagat cagcggttgc tgggctctcg cggatgattc
     1741 cggcattgag gtagacgctc tcggaggaca gcccggggtt cactccggtc gttacctccg
     1801 tctgacagcc acagatcaag agcgtaatca gcgcatcctt gagttgctgg atgggatacc
     1861 gctcccgcag cggggggcgc gttttcagtg tgtggtggct gtggccggtc caggaggaga
     1921 gctgtccatt tttcatggga tctgtcacgg gagcatcgcc gaggctcctt gtggcaatag
     1981 tggcttcggc tacgatccta tttttatcgt ctccgacctt ggcgtaacga tggccgagct
     2041 tccaccggac gtgaagaatc gaatcagtca tcgcgcgcgt gccatcgaga aaacaaaatc
     2101 cttgttgcgg cgcctctcgt ctgcgacggt ttaagctgat cagaggttga tgtcggcggt
     2161 ttgagttggg aaggatggag gacggggcgt ggcgcagcct ggtagcgcac ctgctttggg
     2221 agcagggagt cggaggttca aatcctctcg ccccgaccac aaagtagggt ttagggcgga
     2281 gggtttaggg tggagagacg agacgggatc gctgcggagt tcctttacgc cctataccct
     2341 ggtctttcgc gcctgtagct cagcccggat agagcaacgg cctccgaagc cgtgtgtcgc
     2401 ccgttcaaat cgggccaggc gcaccaaaga atcgttgaag ctgatcgctg gcggctgaaa
     2461 gctgacagca ctcgtatggt gagcgtagct caatggcaga gcactggact gtggctccag
     2521 gggttgaggg ttcgagtccc ttcgttcacc ccaaaaagga agggtttagg gtcaagagta
     2581 gagggtctat cccctatccc ctgttctctt gcgcccatag ctcagctgga tagagcgccg
     2641 gacttcgaat ccgtaggtcg ggagttcgaa tctccctggg cgcaccagaa acagaaaggg
     2701 tttagggtag agggtctagg gttgaggggt gggaagcgaa gatgctggcg tcagacgaca
     2761 agctgaaacg attccggtac gctatacgct ataccctata c
//
LOCUS       DATMXI010000395         5959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_161417, whole genome shotgun sequence.
ACCESSION   DATMXI010000395 DATMXI010000000
VERSION     DATMXI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161417"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VN444_07680"
     CDS             complement(<1..282)
                     /locus_tag="VN444_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWR21721.1"
                     /translation="MPTLTEKNLSSSLDFKVADLSLADFGRKEIEIAEKEMPGLMAVR
                     EKYGKLKSLKGVRITGSLHMTIQTAVLIETLVDLGADVRWASCNIFSTQD"
     gene            complement(524..1615)
                     /gene="lptG"
                     /locus_tag="VN444_07685"
     CDS             complement(524..1615)
                     /gene="lptG"
                     /locus_tag="VN444_07685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HWR21722.1"
                     /translation="MPLNTAMRILDRYLVREFLGTFVFSLAVLLALSAIVDLFDRLSR
                     FLDVSGMVVLQYYVHRLPWFGFQAMPVAVLLAALFSLGRMVRHNELLAMKMGHLSSLR
                     IVAPLLVLGLVVSLAALILGESMIPQMNERALNTYRAKVQKVSPFQRTRDNDIWYRAK
                     GNRFLHISLLEVASGYIHGLTIFELSPDFRLLRRIDAKEAQWQGGRWWLRNGEISRTR
                     PDGSHQVAPFTNLTLDLEEKPMDLAQVVRDSEEMSSAELRDYIERLAKSGMNSVRYQV
                     DLAAKGSTAFMSLVMALIGIAFALRTGKRGVMAWTGACVVVAVCYSILNSLSISLGRG
                     GVLPPMVAAWLPNALFTAAGLGSVLTIKS"
     gene            complement(1664..2965)
                     /gene="lptF"
                     /locus_tag="VN444_07690"
     CDS             complement(1664..2965)
                     /gene="lptF"
                     /locus_tag="VN444_07690"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWR21723.1"
                     /translation="MQHSHRAPRTSPTPRTNGTTVFTGQLRPFSLIDRYLLREMAASF
                     ALGLVTFTFVLLMDQMMRLMDLIINKGAPVLVVLRLILYVLPFSLTVTIPMSVLLAVL
                     ATYGRVSSEGEAIVLKTSGLSLYRLMAPAVLFGIVATLATLWISALVQPDSTRAFKTL
                     THQLFQTKALTALEEGVFNTEYQGLAIYVDRLKKQDGTLQGILVIDRRSQTDQHLVIA
                     REGKLLTRNGEAETPIGLQLSKGNLHISSRDDPGRYRNLDFETYDLQVPTGGTLAEAV
                     GRVRQGKEMNLGELRAQIARLNKEGGKAAWPLQVELHKKFALPIACLILSAIGAPLAI
                     RIKKASRGVSLALSVAIAVFYYILLATGESLGSRGQIEPALGVWFPNLTLGIIAISLV
                     LAEGREAFLPARLRTAIRTLMVFLSRAVSQQKTAKPDRYLT"
     gene            3083..4048
                     /locus_tag="VN444_07695"
     CDS             3083..4048
                     /locus_tag="VN444_07695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWR21724.1"
                     /translation="MGIVVVIIGIVMLAVTLIAMMKKLTRGGVGAWLAERPLRIWWLP
                     FLMLAYYSLMSVAVDQWGFNQFARLAVYFGLPTLLLYLTGPKSDDTFTGRDLVINFAV
                     ALWIWLLIELKIVDTNWLRVQIGSTRPTALPLGVYAAVIYTLITLSGWRRFDLKCDLS
                     FKKSDLLPTAATFGVLSVTLLSITLSTGLTTWGIAKVVRPNPLGLPLAIAIPVAVFVA
                     VPMIFVSIGVVEEILFRGAIQNLLQHRLHPIGALVIASAVFGLAHVNKRALGFDVPNW
                     PYAGVATIAGLGYGFVFWKTNSIMASATVHAMIDAMWVLFLRGGK"
     gene            4187..5872
                     /locus_tag="VN444_07700"
     CDS             4187..5872
                     /locus_tag="VN444_07700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWR21725.1"
                     /translation="MHRRGIGIIVLVGSLVILSEKVTWAQVGPERSTAMKQTAERVKA
                     FAERIPESARNMLSSGALNLINLAERWDRIESHLRPARPASGPSDQPIVSPDVEEALA
                     DQILAPKQVSNPAIDVSSSRFAGFTQSETSTAWCGNNVVIGFNDSGSYLETYFSSGGL
                     SFNGVARSTNKGSSFADLGYLNPGPVFTDSLSGDPVLGCSDANTFYYASLFVRATTSD
                     ISVSKSTDGGATFGNPISAASKSLLTHFLDKEWMAVDPTSPNRLFITYTDFDSSGAMC
                     GFDAGSPILRTAIELVRSIDGGVSWGPPVVIREVCDPAGVQGSQVAVGPVGEVYVAWE
                     AFAVSGASEIDIRKSTDNGSTFGPLVKVNDTICAGSCVRLQGNFRSSEFPTLAVDRSG
                     TSTNGSVYIAWNDGRNLTVSDFWVGAYGYADILFSRSTDGGATWLAPVRINNNPEPLS
                     SGLGTDQYQPGIAVDKAGKIGVCFYDRRRDPGNFLIDWECATSKDAGISWKNKKMTKN
                     NFSAVPNQDLLINPSYMGDYDGLASDFTKTNSGFIGAWGDNSLGNPDIKAGKF"
BASE COUNT         1302 a         1550 c         1777 g         1330 t
ORIGIN      
        1 gtcctgggtt gaaaagatat tgcaggaggc ccagcgaaca tccgcaccca gatcgaccaa
       61 ggtttcgatc aacacggctg tttgaatggt catatgcagc gaaccggtta tcctgacgcc
      121 tttgagggat ttcaatttcc cgtacttttc acgcaccgcc atcaggccgg gcatttcttt
      181 ttcagcaatt tcaatttctt tgcgaccaaa atcggccaag gacaaatccg ccactttgaa
      241 atccagcgaa gaggataggt ttttttctgt taacgttggc atgttcattt cctttttatg
      301 aagagtgcga ggcgccccta ttttggccct caccggccgc gcggaacgcg gtgaggggaa
      361 tgtcatttat ttgataaggt cttgcttctg acgggggaag tatacgcagt aacggacttg
      421 caggtcaagg caatattggc cacataaagg gcggcgtttc gctgttagcg atcagctttc
      481 agtagcaatt ctcccatcgt gctcccggtt tagctgaacg ctattagctc ttgatcgtga
      541 gtacggagcc gagaccggca gcggtgaaga gggcgttggg aagccaggct gcaaccatcg
      601 ggggcaggac gccgcctcgc cctagagaaa tgctgaggga gttgaggatc gagtaacaga
      661 cggctaccac cacgcaggcg cccgtccagg ccatcacgcc tcgcttgccg gtgcggaggg
      721 cgaatgcgat ccctataagt gccatcacga ggctcataaa tgctgtagag ccctttgcag
      781 ccaggtctac ctgataccgc acggagttca tcccgctctt ggcgagcctc tcgatatagt
      841 cccgaagctc cgcagagctc atctcttcgg aatctcgcac gacctgggcg agatccatag
      901 gcttttcttc gaggtcaagc gtgaggttcg taaacggagc gacctgatgg ctcccatcag
      961 gcctggtccg ggagatttcg ccatttcgaa gccaccaccg cccgccttgc cattgggcct
     1021 cctttgcatc gatccttcgc agtagcctaa aatctggtga gagttcaaag atggtcagtc
     1081 catggatgta acccgatgcc acctccagta gcgagatgtg caaaaaccgg ttacccttgg
     1141 cccggtacca gatatcgttg tccctggtcc gctgaaaggg ggagaccttc tgtaccttgg
     1201 cccgatatgt gttcagcgcg cgctcgttca tttgagggat catcgactca cccaggatca
     1261 gggccgcaag gctcaccacg aggccgagga ccaagagcgg agctacgatt cgcagggaac
     1321 tcagatgacc catcttcatg gcgagcaatt cgttgtgccg gaccattctc ccaaggctga
     1381 agagggcggc gagaagcacc gccactggca tggcctggaa tccgaaccag ggcagcctat
     1441 ggacgtagta ctggagaacc accatgccgg aaacgtcgag gaagcgggac aggcggtcga
     1501 acaggtcaac aatagcggag agcgcgagga ggactgccag cgagaagacg aacgtcccca
     1561 gaaactctct cacaaggtaa cgatccagga ttcgcatggc agtattcagc ggcaatgtag
     1621 gggcgctgcg tgctgcgccc ttctgggcag ggcaagccct gccctacgtg agatacctgt
     1681 ccggtttcgc ggtcttctgc tgtgagacag cccgcgaaag gaataccatc agagttctaa
     1741 tagccgttcg aagcctggcg ggtagaaagg cctcgcggcc ctccgcaaga accaagctga
     1801 ttgcgatgat gcccagggtg aggttcggga accatacgcc gagggcgggc tcgatctgcc
     1861 cccgggatcc aagactctcg ccggtcgcca gcaggatata gtagaacacg gcaatagcca
     1921 cgctgagggc aaggctgacg ccgcggcttg ctttcttgat gcgaatcgcg agcggggcgc
     1981 ctatcgcgct taggatgagg caggcaatcg ggagagcgaa tttcttatgc agctctacct
     2041 gtagcggcca agcagccttg cctccctcct tgttaagccg tgcaatctgc gctcgcagtt
     2101 ccccgagatt catttctttg ccctgtcgta ctcgcccaac cgcctcagcg agggtgccgc
     2161 cagtcgggac ctgaagatcg taagtttcga aatcgagatt tcgataccgt cctggatcat
     2221 cgcgggagct gatgtggaga tttcccttcg ataattggag cccgatcggt gtctctgcct
     2281 caccattcct ggtgagtagt ttgccctccc gagcgatgac cagatgctga tcggtctggc
     2341 tcctgcgatc gatgaccagg atgccttgaa gcgtcccgtc ctgtttcttc aagcgatcca
     2401 cgtagatcgc aagcccctgg tactcagtgt tgaacacccc ctcctcaagc gctgtgagcg
     2461 ccttcgtttg gaagagttga tgagtcaacg tcttgaaggc cctcgtgctg tcgggctgaa
     2521 ccagagcact gatccacagg gtggcgagcg tggcaacgat gccgaagaga accgccggcg
     2581 ccataaggcg gtacaagctg aggccgcttg ttttgagtac gatcgcctcc ccctcggacg
     2641 agacgcggcc atacgtggcc aggacggcta acaacacgga catcgggatc gtcaccgtga
     2701 gagaaaaggg gaggacgtag aggatgagac gaaggacgac caaaacggga gcccccttat
     2761 tgatgatcag atccattaag cgcatcatct ggtccatcag cagcacgaag gtaaaggtca
     2821 cgagtccgag cgcgaaggag gctgccatct ctctcaacag gtatcgatcg atcagggaga
     2881 aggggcgcag ttgcccggtg aaaacggttg tcccgttagt gcgaggggta ggcgaagtgc
     2941 gcggtgcgcg gtgcgaatgt tgcatcctac aaagtgtagt gaagagacct cgccaagtaa
     3001 atagaattcg cttgaaatag cttcgacgag gctgttataa aggcgcgtag aagagagcgc
     3061 cgatactatg ggaggttcct gaatggggat cgtggtggtc atcatcggca tcgtcatgct
     3121 ggcagtgacg ctcatcgcga tgatgaaaaa attaactcga ggaggcgtag gcgcttggct
     3181 tgcggaaagg cctcttcgca tctggtggct tccgtttctc atgcttgcgt attacagctt
     3241 gatgagtgta gcggtcgatc agtggggctt caatcaattc gcacggctgg cggtatattt
     3301 tggattgccg acattgctgc tctacctgac cggaccaaaa tctgacgata cattcaccgg
     3361 aagagatctg gtgattaatt ttgccgtagc gctgtggatc tggttgctga ttgagttgaa
     3421 gatagtggac acgaattggc ttcgcgtgca aatcggcagc acccggccca ccgctctgcc
     3481 gttaggcgtg tacgcggctg tcatttatac actcattacc ctgtcgggat ggagaagatt
     3541 tgatctgaag tgcgatctat cttttaaaaa gtccgatctg ctcccgacgg cagcaacctt
     3601 cggagttctc agtgtcacgc tgttgtcgat tacgctctct accgggctga ccacatgggg
     3661 gatcgccaag gtggtgagac ccaatcctct tggcttacct ctggccatag ccattccagt
     3721 tgcggtattc gtcgctgttc cgatgatctt tgtcagtatc ggagtggtcg aagaaatcct
     3781 gtttcgtggc gcgatccaga atcttctcca gcatcgtctt catccgattg gggcactcgt
     3841 tattgcgtct gcagtgtttg ggctggcgca tgtgaataag agggcattgg ggtttgatgt
     3901 cccgaactgg ccttatgccg gggtagccac tattgccggt ttaggatatg ggtttgtatt
     3961 ttggaagacg aactctatca tggcatctgc cacggtccac gccatgatag acgcgatgtg
     4021 ggttctcttt ctcaggggtg gaaaatgagc aactggtgct tgcaggaagc gaggcgattg
     4081 ccggggaggg ggtgattcgc gagggaagaa aaggcgagtg atcgatggtt cccaatattg
     4141 ttcagtggta gatctgaacc tcgaagagga aaaggagcct gacataatgc atcggcgagg
     4201 gataggaatc atcgttttgg tgggtagtct cgttatcctg agcgagaagg ttacatgggc
     4261 ccaggttggc ccggaacggt cgacagccat gaagcagacg gcggaacggg ttaaagcctt
     4321 cgcagaacgc attccagaat ctgcgcgaaa catgctctca agcggcgctc tcaacctgat
     4381 taatctggcg gagcggtggg atcggatcga atcgcacctg cgaccggcgc gaccggcgtc
     4441 cggtccctcg gatcaaccga tcgtatcgcc tgacgtggaa gaggctttgg cggatcagat
     4501 cctggcaccc aagcaggtct cgaacccggc catagacgta tcgtcctctc gctttgcagg
     4561 gtttacccaa agtgaaacct cgaccgcatg gtgcgggaac aatgtggtga tcggctttaa
     4621 tgactcgggg agttatctgg agacatactt ttcatctgga ggattgagct ttaatggggt
     4681 cgcccgctcg accaataagg gaagttcgtt tgccgactta ggctacctga accccgggcc
     4741 ggtgtttacc gactctctct cgggagaccc ggtccttggt tgtagcgatg cgaatacgtt
     4801 ctactacgcg tcgctcttcg tcagggcgac aacaagtgac atctcggttt caaagtcgac
     4861 tgacggtgga gcgaccttcg gtaatccgat ctcggcggct agtaaaagtc ttctcacaca
     4921 ttttctggac aaagagtgga tggctgtaga ccccacgagc ccaaaccggc tcttcatcac
     4981 ctatacagac tttgactcct cgggcgcgat gtgcggattt gacgcaggta gccccatctt
     5041 gagaacggcc atcgaactgg tccgctcgat agatggaggg gtgagctggg gccctcctgt
     5101 ggtgatccgt gaggtctgcg atcctgcagg cgttcagggc tcacaggtag cggtcgggcc
     5161 cgtcggcgag gtctatgtcg catgggaagc gttcgcggtt tcgggcgcca gcgagatcga
     5221 catccggaag tccacggaca acggttcgac gtttggacct ttggtgaagg tgaacgacac
     5281 gatttgtgcg ggaagctgcg tgcgtctaca gggtaacttt cgctcatctg aattccccac
     5341 actggctgta gaccgatcgg gtacctcgac gaacggctcc gtctatatcg cctggaatga
     5401 tggtcgaaat ctgacggtgt cggatttttg ggtaggggct tacgggtatg ctgacatcct
     5461 gttcagccgc tcaacggacg gcggcgcgac ctggttggct cctgttcgga tcaataacaa
     5521 ccccgaaccg ttgtcgtccg gcctgggaac cgaccagtat cagccgggga tcgccgtgga
     5581 caaagcgggg aagatcggcg tctgtttcta tgatcgacgc cgggaccccg gtaattttct
     5641 gatcgactgg gaatgcgcca cctccaagga tgctgggatc agttggaaaa ataagaagat
     5701 gacaaagaac aatttctcag cggttccgaa ccaggatttg ttgataaatc ccagttatat
     5761 gggggattac gacggcctgg ccagtgattt tactaaaacg aacagcggtt ttatcggcgc
     5821 ctggggggat aactccctcg gtaacccgga tatcaaggct gggaaatttt aggggctcgc
     5881 tcgacaagag gcgatgacgg ccgcgaggat atcggcttca aggtggccgg ttgaagcgag
     5941 cggccaccat ggtgaaggc
//
LOCUS       DATMXI010000396         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_160859, whole genome shotgun sequence.
ACCESSION   DATMXI010000396 DATMXI010000000
VERSION     DATMXI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160859"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="hemE"
                     /locus_tag="VN444_07705"
     CDS             <1..1437
                     /gene="hemE"
                     /locus_tag="VN444_07705"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWR21726.1"
                     /translation="SVYRWALPEDCGPLRSAIKAITLREVDLVLFTTAVQVDHLLQVA
                     AESGLEESLRAGLRDAVVASIGPTCSRAMREHGLVVDLEPEHPKMGPLILTVARHAHV
                     LRRIKQARVVRDAGPVPAGSKRGCEVREDQGAGLLQESAFLKACRLEPTPYTPIWIMR
                     QAGRYLQEYREIRGKLSFLELCHRPDLAAEVAVTAVERLGVDAAIIFGDILLVVEPMG
                     VGLEFTKGDGPVIHNPVRTGADLKRLQPVDVQESLSFLFEAVRLARAALPPNIPLIGF
                     AGAPFTLASYLIEGRGSRQYQHTKTLMYRDPGAWHALMERLSEVVSGYLNGQIAAGAQ
                     VAQLFDSWVGCLSPDDYREFVLPHTKRVIANLTPGVPVIHFGTDTATLLPLIREAGGN
                     VIGLDWRVDLGEAWSRLGHEVGVQGNLDPMVLLAEPDEIRRQAGRILERAAKRPGHIF
                     NLGHGVLPQTPVDHLRALIDYVHEETAR"
     gene            1474..2406
                     /gene="hemH"
                     /locus_tag="VN444_07710"
     CDS             1474..2406
                     /gene="hemH"
                     /locus_tag="VN444_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWR21727.1"
                     /translation="MTPFDAVLMIAFGGPTKPEEIRPFLSNVLRGVPVPPGRLEEVAR
                     HYEQLGGRSPITDLTFRQAKSLAALLERQGPKLPVYVGMRYWHPMTSETVEQMVHDHV
                     NRAVGLIMAAHDSGAASWEKSVRAVTDALSVAGPRAPHVDFAQPCYNHPDFIAAVAEQ
                     IRLQLQEIPPALRCNTPLLFTAHSIPSSMAASSPYVQQLHESCRLVAEALGHPDWSLA
                     YQSRSGDPRQPWLTPDVRDVLRTLKAKGCRSVVLAPIGFVCDHVEVLFDLDVEAKAEA
                     DALGLELKRANAVNDHPLYIRALADLVRQRVNQG"
BASE COUNT          464 a          767 c          870 g          486 t
ORIGIN      
        1 tccgtctacc ggtgggcatt gccggaggac tgtggccctc tgcggagcgc aatcaaggcc
       61 attaccctac gggaggtcga tctggtcctc tttaccactg cggtgcaggt ggatcatttg
      121 ctgcaggtcg cggcggaatc cgggttggag gagtcgctgc gggcaggact ccgggacgcg
      181 gtcgttgcgt cgattggccc cacctgcagc agagccatgc gcgagcatgg cctggttgtg
      241 gacctggagc cggagcatcc gaagatgggg cccctgatcc tcactgtcgc gcgccatgca
      301 cacgtactac gacggatcaa gcaggcaaga gtggtgcggg atgcggggcc tgtccctgca
      361 ggctctaagc ggggatgcga ggtgcgggag gaccaaggcg caggattgct gcaagagagt
      421 gccttcctga aagcctgccg cctggagccg accccctaca caccgatctg gatcatgcgg
      481 caggccggac gttacttgca ggagtatcgg gagatccgcg gcaagctctc gtttctggag
      541 ctgtgtcacc ggccggatct ggcggctgag gtcgcggtca cggccgttga acgccttggg
      601 gtggatgccg cgatcatctt cggggacatc ttgctggtgg tggaaccgat gggggttggc
      661 ctcgagttta cgaagggcga tggcccggtc atccacaacc cggtgagaac cggggctgac
      721 ctgaaaaggc tacagccggt cgatgtgcag gaatcgctgt cgttcctctt cgaagcggtg
      781 cggctggcgc gggcagccct tccgccgaac attcccctca tcgggttcgc cggagcgccg
      841 tttaccctgg cctcgtacct gatcgagggt cgcgggtcgc gtcagtatca acacaccaag
      901 acgctcatgt accgcgaccc tggggcctgg catgcgctga tggagcggct atcggaggtt
      961 gtatccggct acctgaacgg ccagatcgct gcgggggccc aggtggcgca attgtttgat
     1021 agctgggtcg gctgtctcag cccggatgac taccgcgagt ttgtcctgcc ccacacgaag
     1081 cgcgtcatcg cgaatctgac gccaggcgtg ccggtcattc actttgggac cgatacggcc
     1141 acgctgctcc cgctgatacg ggaggcgggc gggaatgtta tcggactgga ttggcgtgta
     1201 gacctgggtg aggcttggtc gcgccttggg catgaggtcg gggtacaagg caacctggac
     1261 ccgatggtgc ttcttgccga gcccgatgag attcgtcgcc aggccggtcg gattcttgag
     1321 cgcgccgcaa aacgtccggg ccacatcttc aacctcggtc acggggtgtt gccacaaacc
     1381 cccgtggacc acctgcgcgc gctcatcgac tatgttcacg aagagacggc gagatagcgt
     1441 ctattgcgtc tgtcgcgatg acgctatgac gcgatgacac cattcgacgc cgtcctgatg
     1501 atcgcctttg gaggccctac caagcctgaa gagatccgtc catttctgag caatgtgctt
     1561 cggggcgtac cggtgccacc gggacgcctg gaagaggttg ctcggcacta cgaacaactt
     1621 ggcggtcggt caccgatcac tgatctgacc ttccggcagg caaagagcct ggcggcgctg
     1681 ctggagcgac aggggccgaa acttcccgtg tacgtcggga tgcgatactg gcaccccatg
     1741 accagcgaga cggtcgaaca gatggtccac gatcacgtga accgcgctgt cgggctcatc
     1801 atggccgcgc acgattccgg ggcagcgagc tgggagaaat cggtgagggc ggtcaccgac
     1861 gcactgtcag ttgcagggcc cagggcgcca cacgtggact tcgcccagcc ctgctacaat
     1921 cacccggatt tcatcgcggc ggtggccgaa caaatccgcc tgcaattgca agagatcccg
     1981 cccgctcttc gttgcaatac gccgctactc ttcacggccc atagtattcc atcctccatg
     2041 gccgcatcgt cgccatacgt tcagcaatta catgaatcgt gccgactcgt ggcggaagca
     2101 ttggggcacc cggactggtc actcgcctat cagagccgca gcggcgatcc acgtcaaccg
     2161 tggctgacgc ctgacgtgcg cgatgtcctg cggacgctca aggccaaggg gtgccgatcg
     2221 gtggtgctgg ctcccatcgg atttgtgtgc gatcacgtcg aggtgctgtt cgatctggac
     2281 gtagaagcga aggcggaggc tgatgcgctg gggctcgagc tcaaacgggc caacgcggtg
     2341 aacgatcacc cgctgtacat ccgggcactg gcagatctgg ttcggcaacg cgtgaaccag
     2401 ggttagagac atagaggcag tggatggcgg gagatccgag taacggcctg aagcgtatcg
     2461 tggtggtcgg gggcggcatc gcgggtctcg ccgctgcata ccgtctacag gagcggcaca
     2521 gcagtgaacg ggcgccccgc gaggtactgc ttctggaggc ggactcgcgc cccggcggcg
     2581 tgatcgg
//
LOCUS       DATMXI010000397         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_7952, whole genome shotgun sequence.
ACCESSION   DATMXI010000397 DATMXI010000000
VERSION     DATMXI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7952"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..393
                     /locus_tag="VN444_07715"
     CDS             16..393
                     /locus_tag="VN444_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR21728.1"
                     /translation="MTKRILIVDDSNSMRGIIRSALTDGGHDVIEATNGRDALVALDR
                     HSVDVIITDMNMPVMDGITLVKEIRRRPTNRSTPVLILTTECDAAMKQAGKAAGATGW
                     IVKPFNPQQLRQVIIKVLPPQAG"
     gene            416..>2122
                     /locus_tag="VN444_07720"
     CDS             416..>2122
                     /locus_tag="VN444_07720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HWR21729.1"
                     /translation="MEDDDLLQGFFEESAELLAEFEECLLQLETNPDDQELLNRSFRA
                     IHTIKGNSAMLGFDRIAAVAHNVEDLLGRLRKREIPLTRPAADLLLQSADVIKRLFAA
                     ARGEPVDETEGEELRLALHAFLTHGEDSHIAIPAVPETSSQGILVDGEVPKIGELLLE
                     AQAITTTQLDEALRKHKKVGEILVEDRATSAPHVARALEQQVELQHKQDSSTIRVSTD
                     KVDKLINLVGELVITQSIITQTVATLSPERAALLEGAVGQMERNLRELQERVMAVRML
                     PVGTVFRRFSRVVRDLAKECGKTITLQINGEETELDKTVIERISDPMTHLIRNAVDHG
                     IEPPDRRLAQGKPAGGRIRLDAYHQGGSIFIEVADDGRGLDKSKILGKAVEQGLISEN
                     ESLSDDQVYRLIFRPGFSTAEQVTDVSGRGVGMDVVVRNIEGLGGSIAITTEAGRGTR
                     FTIKLPLTLAIMDGLSIQLGDEVYIIPLISITESIRPKPADLSSVVGRGEVVSVRGQA
                     LPIIRLYEQFDVIPRVTDPTQGLLVIVEREGRRVALLVDELLGQQQVVIKSLETNYRK
                     VPG"
BASE COUNT          458 a          584 c          697 g          383 t
ORIGIN      
        1 ggctaggagg tgggaatgac aaagcggatc ttaatcgtcg acgattcgaa ctcgatgcgg
       61 gggattattc gctctgcgtt gacggatggg ggacacgacg tgatcgaggc tacaaatggg
      121 cgggatgctc tcgtcgcgct cgatcggcac tcggtggacg tgatcattac cgacatgaat
      181 atgccggtta tggacggcat cacccttgtg aaggaaatcc gccgacggcc aaccaacagg
      241 tccacccccg tcctgattct gacgaccgag tgcgacgccg cgatgaagca ggcgggcaag
      301 gcggctgggg cgaccggctg gatcgtcaag ccgttcaacc cacagcagct tcggcaggtc
      361 attatcaagg ttctgccgcc ccaggcggga taggccaagg cgaaggaggc gcagcatgga
      421 agacgatgat ctcctgcaag ggttctttga ggagtcggcc gagttgctgg ccgagtttga
      481 ggagtgtctc ttgcaactgg agaccaatcc tgacgatcag gagctcctga ataggagttt
      541 ccgcgccatc cacaccatca agggtaacag cgcgatgttg ggatttgatc ggatcgccgc
      601 ggtggcgcac aacgtggaag atcttctcgg ccgcctccga aagcgcgaaa taccgcttac
      661 ccgtcccgcc gccgatctgc tcctgcaatc cgcggatgtc attaagcggc tctttgccgc
      721 ggcaagaggt gagccggtcg acgagacaga gggcgaagag ctgaggctgg cgctgcacgc
      781 attcctgacg catggagagg acagccacat agcgattccg gcggtacctg agacaagttc
      841 gcaaggcatc ctcgtggatg gagaggttcc taagatcggc gaactgctgc tcgaagcgca
      901 ggcgattacg acaacgcaac ttgatgaagc tcttcgcaag cacaaaaagg tcggcgagat
      961 cctggtggag gaccgggcga cgagtgctcc gcacgtcgcg cgagccctcg agcagcaggt
     1021 ggagttgcag cacaagcagg actcctcgac gatccgcgtc agcaccgaca aggtggataa
     1081 gctgatcaat ctggtcggcg aactggtcat cacccaatcg atcatcaccc agaccgtggc
     1141 gaccctcagc ccagagcggg ctgcgttgtt ggagggggcg gtaggccaga tggaacggaa
     1201 cctgcgggag ctgcaagagc gggtcatggc cgtccggatg ctgccggttg gcaccgtatt
     1261 cagacgattt tctcgagtgg tacgcgacct ggcgaaggag tgcgggaaaa cgattacgct
     1321 ccagatcaac ggcgaggaga cggaactgga caagaccgtc attgagcgaa tcagtgatcc
     1381 gatgacccac ctgatccgca atgcggtcga tcacgggatt gagcccccgg atcgacggct
     1441 ggcccagggt aaacccgcgg ggggacggat ccggctcgat gcctaccatc agggcggcag
     1501 catcttcatt gaggtggcag atgacggtcg cggtctcgat aaatcaaaga ttctcgggaa
     1561 ggctgttgag caaggcctca tcagcgagaa tgagtcgctc agcgacgacc aagtgtaccg
     1621 gctgatcttc cgacccggtt tctccactgc ggaacaggtg accgacgtat ccggccgcgg
     1681 ggtagggatg gacgtcgtgg tgcgaaatat tgagggactg ggagggtcga tcgccatcac
     1741 gaccgaagcg ggccgcggga cccggttcac gattaagctc ccgcttaccc tggcgatcat
     1801 ggacggcctg tcgatccagc tcggggacga ggtctacatt atccccctga tctcgattac
     1861 cgaatcgatc cggccgaagc cggcggatct gagcagcgtg gtcggccgcg gtgaggtcgt
     1921 cagcgtccgg ggtcaggccc taccgattat ccgcctctat gaacagtttg acgtgatccc
     1981 gagggtgacc gatccgaccc agggcctgct cgttatcgtt gagcgggaag gccggagggt
     2041 cgccctcttg gtggacgaac tgctgggaca acagcaggtg gtgattaaga gtttggagac
     2101 caattatcgc aaggttcccg gc
//
LOCUS       DATMXI010000398         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_294404, whole genome shotgun sequence.
ACCESSION   DATMXI010000398 DATMXI010000000
VERSION     DATMXI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294404"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /gene="dapA"
                     /locus_tag="VN444_07725"
     CDS             complement(<1..571)
                     /gene="dapA"
                     /locus_tag="VN444_07725"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWR21730.1"
                     /translation="MFQGSMVALVTPFKDGRVDESTLRELVEFQIKNGTDVLVPCGTT
                     GESPTLTHDEHRRIIELTVEAANGRIAVVAGTGSNSTTEAIDLTRYAKQAGADGALLV
                     LPYYNKPTQAGLIAHCRAVADAADLPLILYNIPGRTGVNMLPETLAALADHPYIVGMK
                     EATGNLEQMTQDIVLCGDKLSFLSGDDTLT"
     gene            complement(696..1514)
                     /gene="dapF"
                     /locus_tag="VN444_07730"
     CDS             complement(696..1514)
                     /gene="dapF"
                     /locus_tag="VN444_07730"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWR21731.1"
                     /translation="MDKLIFVKMSGSGNDFIMIDNRDGRLAIEPRTLAERICRRRISV
                     GADGLILVESSSKADFRMRILNADGSEAEMCGNGARCVARFAEMLGIAGPHMTFDTLA
                     GIIQAQVIGSRVKLQMSRPQQIRLDQSIEVDGAMHQIHSINTGVPHAVLLCSDLETIP
                     VRALGRKIRFHPTFQPAGTNVDFVAALDERTLAIRTYERGVEDETLACGTGTVAAALV
                     AAALGMVSSPVEVRARSGETLTVSFTRNGTEFHEVFFEGEVRLIYQGELMADAL"
     gene            complement(1727..2983)
                     /gene="lysA"
                     /locus_tag="VN444_07735"
     CDS             complement(1727..2983)
                     /gene="lysA"
                     /locus_tag="VN444_07735"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWR21732.1"
                     /translation="MHHFQYRDDHLMCEEVSIERIAEEVGTPFYLYSHATLAHHFQVF
                     DQAFADIPHLVCFAMKANSNVATLKLFADLGSGADVVSGGELYRALRAGIPSSRIVFA
                     GVGKTREEMGFALKSDILMFNVESPQELRLLNDVAGAMGTKARVALRINPDVDPNTHP
                     YIATGLKKSKFGIDISLAMEEYQVAKELPHIEVVGVHQHIGSQITEIAPFVDSLVKVA
                     DLIAKLRDHGFAIKYLDVGGGLGITYKDEDPPVPRVFAEAMIAVIKDLGCTIVLEPGR
                     VIVGNAGVLVTKVLYDKRTPAKHFIVVDAGMNDLIRPSLYGSFHSILPARQEAGRETV
                     VADVVGPVCESGDFLAKDRSMPAPEPGELLVVMSAGAYGYTMSSSYNSRPRPPEIMVK
                     GDRYFTIRERESYEDLIRGERVPQDH"
BASE COUNT          679 a         1025 c          913 g          663 t
ORIGIN      
        1 gcgtcagagt atcatccccg gataggaacg agagcttgtc gccgcacagg acgatgtcct
       61 gggtcatctg ttccaggttg cctgtcgcct ccttcatgcc aacgatgtat ggatgatctg
      121 ccagcgccgc cagcgtctcc ggcagcatgt tgacccccgt ccgcccaggg atattgtaga
      181 gaatcaacgg caaatccgct gcatcggcaa cagcgcggca gtgagcaatg agacccgcct
      241 gggtcggctt gttgtagtac gggagaacca ggagggcgcc atcggccccg gcctgcttgg
      301 catagcgggt cagatcaatc gcctcggtcg tactgttcga gccggtcccg gcgacgaccg
      361 cgatgcgccc attcgccgcc tcaaccgtca actcgattat ccgcctgtgt tcgtcatggg
      421 tgagagtggg agactcgcct gttgttccgc aaggaaccag gacatcggtg ccgttcttaa
      481 tctgaaactc caccaattca cggagggtcg actcatcaac ccgtccgtct ttaaatgggg
      541 tcaccaatgc caccatcgag ccctgaaaca ttctcttcat gtgacctcca aatccctccg
      601 cacccccctt tcataaaggg gggactgggg gggatttcgt accacgcacg acttacctct
      661 aaaagccttc agccttcagc ctatccacct tgtagttaca gcgcatccgc catcagttcg
      721 ccctggtaga ttagccgaac ctccccctcg aaaaagacct cgtgaaattc cgtcccattc
      781 ctagtaaacg atacagtaag cgtctcgcca ctcctggccc tgacctctac cggggaagag
      841 accatcccca atgcggccgc aaccagcgcc gcggcgacgg tccctgtacc acaggccagc
      901 gtttcgtcct cgacgccgcg ttcataggtc cgaatggcca gcgttcgttc gtcaagggcg
      961 gcgacaaaat cgacattggt ccccgccggc tgaaaggtcg gatggaatct gatcttgcgg
     1021 cccagggctc tgaccgggat cgtttccagg tccgagcaca ggaggacggc gtgaggaacc
     1081 cctgtattga tactgtgaat ctggtgcatg gcgccatcga cctcgatcga ttgatcaagg
     1141 cggatctgct gtggccggct catctggagc ttgaccctgg aaccaatcac ctgggcctgg
     1201 atgatcccgg ccaacgtgtc aaaggtcatg tgtggtccgg cgatccccag catctcggcg
     1261 aacctggcga cgcagcgggc gccgttaccg cacatctccg cctcgctgcc gtcggcattc
     1321 aagatccgca ttcggaaatc cgccttcgac gacgattcga ccaggatcag cccatcggct
     1381 ccaaccgata tccgcctccg gcagatccgc tcggctagcg tcctgggctc gatagcgagt
     1441 cggccatcgc ggttatcgat catgatgaaa tcgttcccgc tcccactcat cttcacaaag
     1501 atcagcttat ccatctatac aaccagggta tagggtttag ggtagagggt gaagcgtgaa
     1561 acagggtgtg ggcggcggtg tgcgacgcgt cgtcgatggc aggcgaagca aggacagcga
     1621 agcctgccgg cgccgatttg agccgtacac ggacgtacgg cgagattaag cagagcgcgc
     1681 cgtcgttcac accgtaaacc atatgcctca taccctcgca ctgttcttag tggtcttgtg
     1741 ggacccgctc gcctcggatc agatcctcgt agctttcccg ttcgcggatc gtgaagtagc
     1801 gatctccctt caccataatc tccgggggtc ggggtcggct attgtagctg gaggacatcg
     1861 tatagccata ggcgccagcg ctcatcacga ccaacagttc accgggctcc ggggccggca
     1921 ttgaccggtc cttggccaga aaatctcccg actcgcatac gggacccacc acatcggcca
     1981 ccaccgtctc cctccctgcc tcctgccgcg cgggcaggat ggaatggaat gagccgtaga
     2041 gactgggacg gatcagatcg ttcatcccgg catcaaccac gataaagtgc ttggcgggcg
     2101 tgcgcttgtc gtagagtacc ttcgtcacca ggacccccgc attcccaaca atcacccggc
     2161 cgggctccag cacgatggtg cagcccaaat ccttaatgac tgcaatcata gcttccgcga
     2221 agacccgtgg cacaggtgga tcttcatcct tataggtgat cccgagcccg ccaccgacat
     2281 ccaggtactt gatcgcgaac ccatgatccc ggagcttggc gatcaggtcc gcaaccttga
     2341 ccaggctgtc tacaaagggc gcgatctcgg tgatctggga cccgatatgc tgatggaccc
     2401 caacgacttc gatgtggggc agttccttgg cgacctgata ctcctccatg gccaaggaga
     2461 tatcgatgcc gaacttgctc ttcttcagcc cggtggcgat atacggatgc gtgttcgggt
     2521 cgacgtccgg gttgatccgt agcgccaccc tcgctttcgt ccccatggcc ccggctacgt
     2581 cattcaacag gcggagctcc tggggagact ccacattaaa catcagaata tcggacttca
     2641 gggcgaaccc catctcctcg cgggtcttgc ctactcccgc aaagacgatc cgactcgacg
     2701 gaatgccagc ccgcaaggca cgatagagct ccccacccga tacgacatcg gctccgcttc
     2761 cgaggtcggc gaagagcttc aaggtcgcga cgttggagtt cgccttcatc gcaaagcaga
     2821 ccaggtgggg gatgtcggcg aacgcctggt caaagacttg gaagtggtgc gcgagtgtcg
     2881 cgtggctata cagatagaac ggggtgccaa cctcctcggc tatccgctcg atcgacacct
     2941 cttcgcacat gagatgatcg tcgcgatact gaaaatgatg catgcagcac tctcctcgtg
     3001 agtagttgtg catcgaattc tggcacaggg ggcgcgagga gtcaaaagga aagtgggggt
     3061 aggtgccgtc agcctaaacg cctaagtagc acgaaaacac gtatagtgtg caaggagcga
     3121 tccactctca acgacgctcc aggctcaatt tgagcgaatg ctcgtccagg ttggtcggga
     3181 tgtttcgcgg ggccgaccta ctcaactcgt ttgatccgtc gggttacgct gcgctaaccc
     3241 gacctacgcg actggtgctt acccctgcac gacccggacc
//
LOCUS       DATMXI010000399         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_228394, whole genome shotgun sequence.
ACCESSION   DATMXI010000399 DATMXI010000000
VERSION     DATMXI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228394"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VN444_07740"
     CDS             <1..385
                     /locus_tag="VN444_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWR21733.1"
                     /translation="KEIGWYPIRLTAAARHDQLFGGFPESIEVFEWHGEYFDTPPGAV
                     SLASSALYECQAFSLGRNVYGLLFHLEVTASMVSEWVRTFTQELDGVKDYIRPDAILA
                     QLPVRIDTLNGLARTLFMRFCQSLR"
     gene            complement(382..738)
                     /locus_tag="VN444_07745"
     CDS             complement(382..738)
                     /locus_tag="VN444_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWR21734.1"
                     /translation="MDRESPAPKTESLDKSDRGTRGAQEPFEEPVTLPTEDVLDLHAF
                     APKEIPSVVEEYIEQCREAEISEVRLIHGKGTGTQRAVVQHLLGGHPGVISFTDAPPE
                     AGGWGATIVRLRTSES"
     gene            complement(785..1255)
                     /locus_tag="VN444_07750"
     CDS             complement(785..1255)
                     /locus_tag="VN444_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR21735.1"
                     /translation="MLPKYQKLLVTTDLSPLGNTAVLHAYGILAERGGTVILWCAVDV
                     SGMPDPLYAQPTPGETLAEERAEIREKLFSSLEALVPEEVRAEGKVTTKIRVVEVSGP
                     IHDTICQEAAAQEVDLIVMASHGYSGMKHLLLGSVVEHVVRLADRPVLIIRSRE"
BASE COUNT          410 a          631 c          600 g          486 t
ORIGIN      
        1 caaggagatc ggctggtacc cgatccggct caccgcggcg gcgcggcacg atcagctgtt
       61 cggcgggttc cccgagtcga ttgaggtgtt cgagtggcac ggcgagtatt tcgacacgcc
      121 gcccggggca gtcagcttgg ccagttcggc gctctatgag tgccaggcct tctcgctcgg
      181 gcgcaacgtc tatggcctgc tgtttcatct cgaggtcacg gcatcgatgg tctctgagtg
      241 ggtgagaact ttcacacagg agcttgacgg ggtcaaggac tatatccggc ctgatgcgat
      301 ccttgcgcag cttccggtcc ggatcgatac gctcaatgga cttgccagga ccctctttat
      361 gcgcttctgt cagagcctcc gctagctttc tgatgttctc aaccggacga tcgttgctcc
      421 ccatcccccc gcctcgggtg gggcgtcggt gaaggagatg acgccggggt ggccccccag
      481 taggtgctgg acaacggccc gctgtgttcc cgtccctttc ccatggatca gccgaacctc
      541 tgatatctcc gcctccctgc actgctcgat gtactcttcg accaccgacg ggatctcctt
      601 cggcgcgaag gcgtgcaggt cgaggacatc ctcggttggt agggtcaccg gctcctcgaa
      661 tggttcttgc gccccacgag tgcccctatc tgatttgtcc agcgactcag tcttcggcgc
      721 cggcgattcg cggtccatca cccaccggtc gcgccatgac gagcaaagtg agcggcgaga
      781 gtcctcactc ccggctgcgg ataatgagaa cggggcgatc ggctaaccgc accacatgct
      841 ctaccactga gcccaggagc aggtgcttca tcccggaata gccatgggat gccatcacaa
      901 tgaggtccac ctcctgggct gcagcctctt ggcatatcgt atcatggatc ggtccggaaa
      961 cctccactac ccgtatcttc gtggtcacct ttccctctgc gcgaacctcc tcaggcacaa
     1021 gtgcttccag tgaggagaac agcttttccc ggatctccgc ccgttcctca gcgagcgtct
     1081 ccccaggcgt gggctgagcg tagagcgggt ccggcatccc tgatacgtca accgcacacc
     1141 aaaggatgac agtgccgccc cgttccgcca agatcccata ggcatggagg actgcggtgt
     1201 ttccgagggg tgagagatcc gtagtcacca gcagtttctg atattttggc aacatggcat
     1261 gatacctcct cgcgtcaagc agggttacgt tgctatccgc tcactgatat tgtggaccga
     1321 ccggacgact ctacggcgca ccccttacgc tgagcatctt ggggcatatt taagatataa
     1381 tcctcttcgg gtatcgtgtc aataggtgag taagtactct aaaatagtgc tcgttctctg
     1441 cggtgaggag tatgagcgac cccaatatag acattgacat actcgcaggc tcactggcgc
     1501 ccatcgtgag aagtttgagc catcgcagct cagaggatac cccttttcca ttgctcctgt
     1561 tgtgcctcct cgggccagta aaccatcaca aaaagatgtg aggaaccgtc caaggcggac
     1621 cctctccctg gcgtggtctt cgcgttgttc atcaaccccg ccagagagcc attgttaggc
     1681 gaacgagaga gcgtgggtgc gccgtcacat cgccctgtcc aggtactgca tttttgtctt
     1741 gacaataggg tctgcctcag ttaatttgct acacgaagtt tgcctgtccg tggacaggag
     1801 cctcgggtta ctcctgatcg aaggacatgc gacttctacg ctggtgctag ccatcggatc
     1861 caatcaggcg cacagggagg atttccctca gatcgatcta catggagcaa agactcctgg
     1921 gggcggaaga gcgttgcgaa atactctcct tgcgggttcc tggaagaacg ttgcgggccg
     1981 atgcggcaaa actttcgtta ggtttctgag cttcgggatg ttatgctaca ccgggcggat
     2041 ggctacgtcg ttgccatgat cgttccgagc catgttctcc atggatagtt gtaagattga
     2101 cctccatggg atacaagccg gaataca
//
LOCUS       DATMXI010000400         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_74934, whole genome shotgun sequence.
ACCESSION   DATMXI010000400 DATMXI010000000
VERSION     DATMXI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74934"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2440)
                     /locus_tag="VN444_07755"
     CDS             complement(<1..2440)
                     /locus_tag="VN444_07755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWR21736.1"
                     /translation="MPPKGRRPAKAKRAQDPKDAQAYDHEEKLLLRPDVGLQSQFKQK
                     KAPKTYRYDPSLDPALSWDGNADRERAEALIARIEQATDLTEAKAAAAELRRMSRPFL
                     NWTGKAERHDLTVPTLPLFVHERLSTQAILQSVKSHKRDRQQTLALFADEELDVTDRL
                     LRAYEYQTDWVNRLILGDSLVVMNSLLQYEGLGGQVQMMFMDPPYGVKFGSNFQPFIR
                     RRDVKHGDDGDLTREPEMVQAYRDTWELGLHSYLTYLRDRLLLARELLNERGSIFVQI
                     SDENLHHVTELLDEVFGSENRVAVISFKKTGFASDEKISTTHDFLLWYARSRNQAKLR
                     SVFGERELTANELSFYDQIELPDGTRRPLAAEERVLGPRVRELGRPFARNPMVSPGWQ
                     DSLGFGIKFEDHTFRPPANRHWATTKEGIERLRTARRLAIKGNTLRFVRYFDDFALQP
                     LGTVWSDTGVGGFVGDDRIYVVQTDTRVIQRCLLMSTDPGDLVLDPTCGSGTTAYVAE
                     QWGRRWITIDTSRVPLALARQRLLTATFPYYELRDPSRGPSGGFVYTRKQNQKGEEVG
                     GIVPHITLKSIAQNEPPTEEVLVDRPEVESGVVRVSGPFVVEATIPTAIEVEPFPDHG
                     RGQAETVKDREDIYEIDPINRMIEVLRRSPSLRLTGNQTVTLKNIRRPAKAMDLHAEA
                     EVAIPTLEDLAEEAATQKSLDMKRGKPVAFIFGPEHGPVTEQMVFVAAKEAHIKSYTH
                     LFVVGFAIQPGASKLICNCEQVVGLPATYVQATMDLVMSDLLKTTRASQIFSVTGAPD
                     LRLIRLKRKNGNGPL"
     gene            2689..3483
                     /locus_tag="VN444_07760"
     CDS             2689..3483
                     /locus_tag="VN444_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HWR21737.1"
                     /translation="MPDFDAPPVIEVVLGVQFDPLLNFLTAHYGLFSSLVKQEYPILE
                     DRTPLSEIFEGPKGIQSKPEIELLNIPPLRRVFLIHADGNYLIQVQPTRFHHNWRKLR
                     EEDEYPHFSSAKERFLKHWGTFRAFVVDNKLGEIHPNQYEVTYINHIVESEGAFPVAI
                     EEYAPLFSWSSAHSTGFLPNPSGLTTELRFPLPEQRGTLHVTFKHGRRASDNRDVLIL
                     DLTARGAAKPDGSDMEEWFGLAHEWIVRGFTDLTSAVAHKRWRKTQ"
     gene            3480..4001
                     /locus_tag="VN444_07765"
     CDS             3480..4001
                     /locus_tag="VN444_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21738.1"
                     /translation="MTREIGSRFEEWPEGSAATCLLGKEAPTVVSSASTTGSMSAHWV
                     SSDILEEEHFKRLRAVFDKISSFRYLPQGWDSYDAPQIDQVTTTAAQKLIKLLWQSLS
                     TSLPEPFAAPCSDGGILLEWELPTTELSITVGPGGTLLEYLIARKGTDEITDEGATRD
                     LVTLVRRVLVHFV"
BASE COUNT          973 a         1257 c         1258 g         1055 t
ORIGIN      
        1 agagcggccc gttcccattc ttccgcttga gccgaatgag cctgagatcg ggggcgccag
       61 ttaccgagaa gatctggctc gcgcgtgtgg tcttgagcag atccgacatc accaggtcca
      121 tggtggcctg aacgtaggtg gcaggaagtc ccaccacctg ctcgcagttg cagatcagct
      181 tgctggcccc cggctggatg gcaaagccga ccacgaaaag gtgcgtatag ctcttgatgt
      241 gggcctcctt ggctgcgacg aacaccatct gttcggttac cggtccatgc tccggaccga
      301 agatgaatgc aaccggcttg ccccgcttca tgtcgagcga cttttgcgtg gctgcctcct
      361 cagccaagtc ctccagggtc ggtattgcaa cctccgcctc ggcgtgcagg tccattgctt
      421 tcgccgggcg tcggatgttc ttgagcgtga ccgtctgatt gcctgtcagc cgcagcgaag
      481 gggagcgtct caggacttcg atcattcggt tgatcggatc aatctcgtag atgtcctcac
      541 ggtccttgac tgtctctgcc tgtccccgac cgtgatcggg gaatggctcc acctcgatgg
      601 ccgtggggat ggttgcctca accacaaatg gcccggagac gcgcacgacc cctgattcca
      661 cttccggtcg gtccacgagg acttcctctg tgggcggctc gttctgggcg atcgatttga
      721 gcgtgatatg cgggacgatc ccgccgactt cttccccctt ctggttctgc ttgcgtgtat
      781 agacgaagcc gccggatggg ccacgggacg gatcgcgcag ctcgtagtac gggaaggtcg
      841 cagtcaggag ccgttgcctg gctaaggcca gcgggacccg actcgtgtca atcgtgatcc
      901 atcgccgccc ccactgttcc gccacatagg ctgtcgtccc gctgccgcac gtcgggtcaa
      961 ggaccaagtc gcccggatcg gtgctcatca ggagacaacg ttgaatgact cgcgtatcgg
     1021 tttgaacaac ataaattcgg tcgtctccga caaatcctcc cacccccgta tcactccaga
     1081 cggtgccaag aggttgcaga gcaaaatcat cgaagtagcg aacaaatctt aatgtattgc
     1141 ccttaatggc taaacgtctg gccgtgcgta gcctctcaat cccttctttt gtcgttgccc
     1201 aatgtctatt ggcggggggg cgaaaggtgt ggtcctcaaa tttaattcca aagcccagcg
     1261 aatcctgcca gccaggggat accattggat ttctagcgaa cgggcgacca agttctctga
     1321 ctcttggacc caaaaccctt tcctcggcag ccaatggacg ccgagtccca tcaggtagtt
     1381 cgatctggtc ataaaaggaa agctcgttag ccgtcagttc gcgttcacca aagaccgatc
     1441 gcagctttgc ttgatttcgt gaacgcgcat accagagaag gaaatcgtgc gttgtggata
     1501 tcttttcgtc gcttgcgaaa ccagtcttct tgaatgaaat gaccgcaact ctgttctcgc
     1561 tcccaaagac ttcatccagg agttcagtga catggtgcag gttctcatcg ctgatctgga
     1621 cgaagatgct gcccctctcg ttcagcagct ccctcgccag gagcaggcgg tcgcggagat
     1681 aggtgagata cgaatgcagc cccagctccc aggtgtcgcg gtaagcctgg accatctcgg
     1741 gctcgcgggt cagatcgccg tcgtcgccgt gcttcacgtc gcgccgtcgg atgaacggct
     1801 ggaagttgga gccgaacttg acgccataag gcgggtccat aaacatcatc tggacctgac
     1861 cgcccagtcc ctcatattgc agcaggctgt tcatgaccac caggctgtcg cccaggatca
     1921 ggcggttgac ccagtcggtc tgatactcat aggccctgag cagccgatca gtgacatcca
     1981 gctcttcgtc ggcgaagagg gcgagcgtct gctgtcggtc gcgcttatgg gatttgacgg
     2041 attgcaggat cgcctgagta gagaggcgtt catggacgaa gagggggagg gtcgggaccg
     2101 tcagatcatg ccgttccgcc ttcccggtcc agttaagaaa cggccgggac atccggcgga
     2161 gttcggcggc cgcggccttc gcctcggtaa gatcagtagc ctgctcgatt cttgcgatca
     2221 gcgcttcggc tcgctcgcgg tcggcgttcc cgtcccacga cagggccggg tcgaggctgg
     2281 gatcgtagcg gtaggtcttc ggggccttct tctgcttaaa ctgcgactgc aacccgacgt
     2341 cgggtcgcaa gaggagtttc tcctcgtggt cataggcttg cgcgtctttc gggtcctgcg
     2401 cccgctttgc cttcgctggc cttcgccctt tgggtggcat atcctctcct cctgttgccc
     2461 agcttggtca gcgctatcct agacaggtag cccctggctg tcaagccttc cctcccgtaa
     2521 ttgcttgaca gaggtttatc ctgtagataa gctgtcgcac cagaggtggt ctgggcgtgc
     2581 gaactccacg acccagagaa gctttacgcc aagaaaggat tgctcatagg tttgaagagg
     2641 ggtaatctac ttgtcagagg ttaaggaatg agccggtcat tcgaattcat gcctgatttc
     2701 gatgccccgc cagtgatcga ggtagtcctc ggagtgcagt ttgatcccct cctgaacttc
     2761 ttgacggctc actacggttt gttttcgagt ctggtgaaac aggaataccc cattctcgaa
     2821 gacaggacgc cactgtcgga gatattcgaa ggcccgaaag gtattcaatc gaagcccgaa
     2881 atcgaacttc taaacatacc acccctcaga cgggtatttt tgattcacgc cgatgggaat
     2941 tatcttattc aggttcagcc gactcggttt caccacaatt ggcgcaagct acgcgaagaa
     3001 gacgagtatc ctcatttcag ttcagcgaaa gaacggttcc tgaaacattg gggaaccttt
     3061 cgggcgtttg ttgtagataa caagcttgga gagatacatc ccaatcaata tgaggtcacg
     3121 tatatcaacc acattgttga atccgagggt gcgtttccag tggcaattga ggagtatgca
     3181 cctctgtttt cgtggagctc cgctcattcg actggcttcc ttccaaaccc aagcggtctc
     3241 acaacggaac taagatttcc actgccggag cagcgcggga cccttcatgt tacttttaag
     3301 catggtagaa gggccagcga caacagggat gtactgattc ttgatcttac agccagagga
     3361 gcggctaagc cagacggctc agatatggaa gagtggttcg ggctggccca cgaatggatt
     3421 gtgcgtgggt ttactgatct cacatcggct gttgctcata agcgttggag gaaaacccaa
     3481 tgacgagaga gatcggaagt agatttgaag agtggcctga aggcagcgcg gcgacttgcc
     3541 ttctcggaaa agaggcaccc actgtagtgt cctccgcttc aactacgggg agtatgtcag
     3601 cgcattgggt ctcctctgac attctggaag aggagcattt taagagacta cgcgcggtgt
     3661 ttgacaagat ttcttctttc cggtacctgc ctcagggctg ggattcttat gatgctccgc
     3721 aaatagatca agtaaccaca acagcagctc agaagctcat caagcttcta tggcaatctc
     3781 tcagcacatc tctcccggag ccgttcgctg ctccctgttc ggacggagga atacttctcg
     3841 aatgggaatt gccgacgacc gagctcagta ttaccgtggg gccaggtgga acccttctcg
     3901 aatatctgat tgccagaaaa ggaaccgatg agatcactga tgaaggcgca actcgcgatc
     3961 tcgtaacact tgtcagaaga gttttggtcc atttcgtttg accgacaagc gtcttccccc
     4021 ttttgatcca ggttaagaga tcgagcgaat ggaggtccaa gagattgcag atgtcgatgc
     4081 cctttaccgg agaatacacc ccttacaagt caaagatgga acgcctactt cggcagcatt
     4141 taaggatgca gaactgtcag tggatctggc gaggctcacc acttttgaac gaagtcttac
     4201 gggataccca tcacacgggt tggcgtctgt aactgccggc tatgcgagat ctctggagca
     4261 aacggtattg cacgatccgc tggaaaccaa tcctgcacac accttagtga aaggtaacaa
     4321 aacccctcgt atcgcaagaa gcctcgcgcg aagcgccaag tgggaagcgc ttccgccaag
     4381 tcgctaattt actatctctg cacactgctg tccaaaataa cccgcaacaa tcttaagggg
     4441 cacggaattt cctcacgttt tggacgggtt tggcttcacc gcgagttgag gtctcccatt
     4501 ctgctgtcca agattacgca aaggtgaagg cttgctgggg cgt
//
LOCUS       DATMXI010000401         4351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_35652, whole genome shotgun sequence.
ACCESSION   DATMXI010000401 DATMXI010000000
VERSION     DATMXI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35652"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VN444_07770"
     CDS             complement(<1..518)
                     /locus_tag="VN444_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWR21739.1"
                     /translation="MAQVKGLKCRECGRPYPADPLHVCEFCFGPLEVDYDYDALRGTL
                     TRKAIEAGPPSIWRYKALLPIEGEPVVGKHCGMTPLVRAKNLARALGMREVYVKNDTV
                     SHPTFSFKDRVVAVAVTKAVEFGFKVVACASTGNLANSVAAHAAEGGLRSFVFVPSDL
                     EQGKVLGTLIYNP"
     gene            complement(520..756)
                     /locus_tag="VN444_07775"
     CDS             complement(520..756)
                     /locus_tag="VN444_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HWR21740.1"
                     /translation="MPKRMITLIYPQALIKEPVIYRLAREFKLIPNVRRARVTEQTGE
                     VMLELDGEEESLNKGIAYLAGLGIRVEPTPEKGR"
     regulatory      complement(841..964)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1032..1271)
                     /locus_tag="VN444_07780"
     CDS             complement(1032..1271)
                     /locus_tag="VN444_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HWR21741.1"
                     /translation="MAKKRVHLTFTGKLAEEPILWQLSQTFDLVFNIRQADFTEGIGW
                     IMAEIEGDPQPLENGITWLEDHGVHVAPIEQDVVS"
     gene            1571..2233
                     /locus_tag="VN444_07785"
     CDS             1571..2233
                     /locus_tag="VN444_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR21742.1"
                     /translation="MPTEIPTLTPHQVKSALVAGVLLIDLRPHKEFASRHIPGSINVV
                     FSRKSLPERVATAIPPGPSIVLLSGEEGVAEAAADALQRVNHNPLRGIVTGGTEAWDY
                     AGFPLAQLSQVPVADLWQRLNAPNDELMLIDVREPFEWELGYIKGSLLISLAEIWQHA
                     CRLDQHRHIVLICEEGVRSSTAASILLHHGFPRAGNVPGGIGHWLTANYPTIRLPKPP
                     KN"
     gene            complement(2234..3427)
                     /gene="sat"
                     /locus_tag="VN444_07790"
     CDS             complement(2234..3427)
                     /gene="sat"
                     /locus_tag="VN444_07790"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWR21743.1"
                     /translation="MQESIESPGAEALTQILPHGGRLVSRILTGEARADAIGRAKDLP
                     AISLNARAVSDVECLATGVFSPLEGFMDRDDYEGVVNEMRLKNGILWTLPITLAAPKE
                     EAANLREGGEAALLDSDGELLGLLSVQEIFPYDKRAEACLVYATEETRHPGVQYLYQR
                     GELLVGGSISMVRPPSLPGFEDYYCVPAETRRRFKERGWQTVVGFQTRNPIHRSHEYI
                     QKCALELMDGLLIHPLVGRTKLDDVPSEIRLRCYRALEECYFPKERVMLSVFPGAMRY
                     AGPREAVFHALVRKNYGCTHFIVGRDPAGVGGFYHPYAARDLFLRLSRDELDITPLFF
                     DEAFFCRRCDGMASSKTCPHAASERVTLSGTRVRELLRSGEALPEEFTRPEVSEILAE
                     WMQGE"
     gene            complement(3462..4220)
                     /locus_tag="VN444_07795"
     CDS             complement(3462..4220)
                     /locus_tag="VN444_07795"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWR21744.1"
                     /translation="MERGSGIQSAESVVELNERFRAAEPEVVLRWAIDTFAPKLALAS
                     SFGAEDMVLIDMLSKLEPATTIFTLDTGRLHEETYDVMERTRKRYKVAIESYFPGRDA
                     VEALERERGFYSFRQSIEDRKFCCRVRKVEPLGRALLNVDAWITGLRREQAATRTGID
                     AVEIDASHGSIVKINPLVEWTESQVWAYIREHDVPCNALHDQGFPSIGCAPCTRAIKP
                     GEDVRAGRWWWENPETKECGLHLESGHGDHGARG"
BASE COUNT          902 a         1409 c         1149 g          891 t
ORIGIN      
        1 gggttgtaga tcagggtgcc cagcaccttg ccctgctcca ggtcggacgg cacaaagacg
       61 aagcttcgaa gccccccttc ggcggcgtgg gccgccaccg agtttgccag gttgccggtg
      121 gaggcacagg ccactacctt aaaaccaaac tccacggcct tggtcaccgc caccgccacg
      181 acccggtcct taaatgaaaa agtgggatgg ctcacggtat cgttcttgac atagacctcc
      241 cgcatgccta acgcacgcgc caggttcttg gcccttacga ggggagtcat cccgcaatgt
      301 ttcccgacca cgggctctcc ctcgatcggc agcagcgcct tgtatcgcca gatgcttggc
      361 ggtccggcct cgatcgcttt tctggtcagc gtgccccgaa gcgcgtcgta atcgtaatcg
      421 acctcgagag gcccgaaaca gaactcacag acatgcaggg gatcagcggg atatggtcta
      481 ccgcactccc tgcacttcaa tcccttcacc tgcgccatct cagcgtccct tctccggagt
      541 cggctccacc ctgattccta aaccggccag gtaggcaatt cccttattca acgattcctc
      601 ttcgccgtcc agctccagca taacctcgcc tgtctgctcc gtcacccgtg cccgacgaac
      661 attaggaatg agcttaaatt cccgcgcaag acgatagatc accggctcct tgatcagtgc
      721 ctgcgggtag atgagtgtga tcatccgttt tggcatcaca gagtcccttc accgaaatga
      781 aataatgaaa aagccccttc tcagggatgc gagaaggggc tgtcagaatc gacgccatcc
      841 ttctcatctc tcatcccgcc ctgtatccgg gatgtagaag ttggcaccac tgtctaccag
      901 aacatgctgc agaccggttg ccgtggcttc gcagggtcag tccctcagcc actctggatg
      961 aaaaggcaaa tcgcagctat atcatcaagg caacttttcc ctgtcaagca acaatcgaga
     1021 cctcgatctc tttaagagac gacgtcctgc tcgattggcg ccacatgaac accatgatcc
     1081 tccagccacg tgatcccatt ctcaagcggc tgcgggtctc cctcgatctc cgccatgatc
     1141 cacccgattc cttcggtaaa gtcggcctga cgaatattga agaccaggtc gaatgtctgg
     1201 ctaagctgcc acaggattgg ctcctcggcc agcttgccgg taaacgtcaa gtgaacacgt
     1261 ttttttgcca ttacgtcctt cggagatcga tcgtggatca gagatgatag atgcttgggt
     1321 agtagatatt cgacccaatt tcgtttgagc ccttcatgct cggtgaagta taaagggttt
     1381 cgatcggact gtaaagtccc aatacaccct cagggggatc cccctactcg gtcacgaaac
     1441 agccggtgcg ccgctccact cagcaatggc attgacaagt gcagtcaagg tagactacat
     1501 atagtagaga cctcacacgt aggtaagata cgatgcgcaa ggcatgtttc cctcaaccag
     1561 cgtgagcgcg atgccaaccg agataccgac gttgacgcca catcaggtaa agagcgcatt
     1621 ggtcgctggc gtgctgctga tcgatctgcg cccgcacaag gagttcgcgt cacgccatat
     1681 tccgggaagt atcaacgtcg tcttcagtcg caaaagtctc cctgagcgtg tcgcgaccgc
     1741 gatcccaccc ggtccttcga tcgtactcct ctccggcgag gagggtgttg ctgaggccgc
     1801 ggctgatgcg ctccagcggg tgaatcacaa tcctctccgg gggattgtca ctggaggcac
     1861 cgaggcgtgg gactacgctg gattcccgct ggcccaactg tcacaagtgc ctgtcgcaga
     1921 tctctggcaa cgcctgaacg ctccgaacga tgaactgatg ctgatcgacg tgcgggagcc
     1981 gttcgagtgg gagctgggct atatcaaagg ctcactgctc atctcgctcg cagagatctg
     2041 gcagcacgcg tgcaggctcg atcagcatag acacatcgtc ctgatctgcg aagaaggtgt
     2101 gcgcagcagc acggcagcca gcatcctgct tcaccacggc tttccaaggg ccggcaacgt
     2161 cccggggggg attggccact ggttgacagc caactacccc accatccgcc taccgaagcc
     2221 gccaaagaac tgattattca ccctgcatcc attccgccag aatctcggag acctccggcc
     2281 gcgtaaattc ttccggtagc gcctcaccgc tccgaaggag ttcacgaacg cgcgtaccgc
     2341 tcagcgtcac ccgctcggac gccgcatgcg ggcaggtctt ggacgaggcc atcccatcgc
     2401 agcgacggca gaagaacgcc tcatcgaaga agagcggagt gatgtccaat tcgtcgcgtg
     2461 acagccgcag gaagaggtca cgggcggcgt acggatgata gaagccgcct acccccgcgg
     2521 ggtcccgccc aacgatgaag tgggtgcatc catagttctt gcgcaccagg gcgtggaaga
     2581 ccgcttcgcg aggccccgca tatcgcatgg cgccagggaa gacgctcagc atgacgcgct
     2641 ccttcgggaa gtaacactcc tcaagcgcac gatagcatcg aaggcggatt tctgatggga
     2701 catcgtcgag ctttgtccgg ccgaccagcg gatggatcag caggccgtcc atcaactcta
     2761 acgcgcattt ctggatgtac tcgtgcgacc gatgaatcgg attgcgggtc tgaaacccga
     2821 ccacggtctg ccatccacgc tccttgaacc gtcgccgcgt ctcagccggc acgcagtaat
     2881 agtcttcgaa tcccggtaac gacggggggc gaaccatact gatcgacccg cccaccagga
     2941 gctcgccccg ctgatacagg tactgcacac ccgggtggcg cgtctcttcc gtggcgtaca
     3001 ccagacacgc ctccgcgcgc ttgtcgtagg ggaagatctc ctgcaccgag aggagaccaa
     3061 gcagctcacc gtccgagtcc agcaaggccg cctcgccccc ctcccgtaga ttggccgctt
     3121 cctctttggg cgcggccagg gtgatcggaa gcgtccacag tatgccgttc ttcaggcgca
     3181 tctcattgac caccccctcg tagtcatccc gatccataaa cccttcgagc gggctaaaga
     3241 cgccggtggc caggcattcc acgtcggaca cggccctcgc gttcaaggaa atcgcgggga
     3301 gatccttcgc ccgacctatc gcgtcggccc tggcctctcc tgtaagtatc cgagagacga
     3361 gccgcccgcc atgggggagg atctgtgtga gcgcctctgc ccccggcgat tcaatcgatt
     3421 cctgcattgt ctgctctcct gtcgacatcg tgcccctcgc tttagccgcg tgcgccatga
     3481 tcgccgtgtc cgctctccaa atgcagccca cactccttgg tctcaggatt ttcccaccac
     3541 catcgtcccg cccgcacatc ctcgcccggc ttgatagccc tcgtgcatgg cgcgcagccg
     3601 atactgggaa acccctggtc atgaagagcg ttgcagggca catcgtgttc tcggatatac
     3661 gcccagacct gcgattcggt ccactcgacg agcggattga tcttcacgat tgagccatgg
     3721 ctcgcatcaa tctcgacggc gtcgatgccg gtgcgggttg ctgcctgctc acgccggagc
     3781 ccggtaatcc atgcatcgac gttcagtagc gctcgaccga gcggctccac ctttcgaacg
     3841 cgacaacaga acttccggtc ctcgatactc tgccggaagg aataaaaccc gcgctcccgc
     3901 tccagggcct ctacggcgtc tcgccccgga aagtagctct caatcgcaac cttatatctt
     3961 tttcgagtcc gctccatgac gtcgtacgtc tcctcatgca accgaccggt gtccagggtg
     4021 aaaatcgtag tcgccggctc aagcttcgac aacatatcga tcagcaccat atcttcggcc
     4081 ccaaaactgc tggcgagcgc cagcttgggg gcgaaggtgt cgatcgccca ccgaagcacc
     4141 acctcaggct cggccgcccg gaaccgctcg ttcagctcga caacgctctc tgcgctctga
     4201 attcccgatc ctctttccat cattcctcct tattggaata tgtcaacgca cgtgaaatga
     4261 ttcatccgtt cgtgctcgta cttaattgcg ataatacacg ttacgtcatt cccgcaggcc
     4321 ttaagcggga atccattgat tttcctggat a
//
LOCUS       DATMXI010000402         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_161610, whole genome shotgun sequence.
ACCESSION   DATMXI010000402 DATMXI010000000
VERSION     DATMXI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161610"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VN444_07800"
     CDS             <1..266
                     /locus_tag="VN444_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWR21745.1"
                     /translation="SFEAGQSPYGIYDLAGNVWEWVNDWYDKDYYEKSPSRNPNGPEQ
                     GATKVLRGGSWYANLGYLKSTYRFSLPPSSRDSDFGFRCAKTP"
     gene            416..682
                     /locus_tag="VN444_07805"
     CDS             416..682
                     /locus_tag="VN444_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWR21746.1"
                     /translation="MTKTLSLKEARSQFSDIVDRAGRLSERIVVTKNGRPEAVVMGAD
                     EFESWVETLELLSNPKAVKSLKQGLKEAKAGKFHSFKSAFGEEQ"
     gene            679..933
                     /locus_tag="VN444_07810"
     CDS             679..933
                     /locus_tag="VN444_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR21747.1"
                     /translation="MRQVRLTPQALKDLKDYDDPTRERVKEALRHLADHPLDGKPLKG
                     HFQKDKVWSYRVWPYRILYRRIGSEWLDVLSIEHRKDVYR"
     gene            complement(1435..2805)
                     /locus_tag="VN444_07815"
                     /pseudo
     CDS             complement(1435..2805)
                     /locus_tag="VN444_07815"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
     gene            3228..>3664
                     /locus_tag="VN444_07820"
     CDS             3228..>3664
                     /locus_tag="VN444_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWR21748.1"
                     /translation="MGVRQKGQAWLSVLWLLMAVTLVACVAGLPQTPLPEITGKDGAP
                     MMLVPRGEFTMGSNTSFAHEQPLHRVTLDAFYMDKYEVTVGQYTKFLEATSLAAPPDW
                     NVMNQPPHQNRPVVNVNWWDANSYCRWAGKRLPTEAEWEKAARG"
BASE COUNT          814 a          985 c         1066 g          799 t
ORIGIN      
        1 ggtcgtttga agcaggccag agtccctatg ggatctacga tctggcgggg aatgtttggg
       61 aatgggtgaa tgactggtat gacaaggact attacgagaa gagtccgtcg cggaacccga
      121 atggtccgga acagggtgcg actaaagtgt tgcggggcgg ctcatggtac gccaatctgg
      181 ggtacttaaa atccacctac cggttcagcc tccctccgtc tagccgggac agcgatttcg
      241 ggttccggtg cgccaagact ccgtaggtgc atgccgtaag tcactggcca ccatgtcaga
      301 tcctatttcc tctcctcaaa caggaaattc agaactcccc tattctcaga cttccaagca
      361 cggattgcca aagggggcat aattctgtac actctttaca cgcaaggaga tcggcatgac
      421 taagacactt tcgttaaaag aagcgcggag ccaattctcg gacatcgtcg acagagcggg
      481 acggctttca gaacggattg tggtgacgaa gaatggacgg ccggaggcgg tggtgatggg
      541 agccgacgaa ttcgaaagtt gggtggagac attggaactg ttgtccaacc cgaaggcggt
      601 caaatccctc aagcagggac tgaaagaggc caaagctggg aaatttcact ccttcaaaag
      661 tgctttcggt gaggagcagt gaggcaagta cgtctcaccc cgcaagctct caaagacctc
      721 aaggattatg acgaccccac gcgagaacga gtgaaagagg ccctccgcca cctcgccgat
      781 catcctctcg acggcaaacc gctgaaaggc cattttcaaa aggacaaggt ttggtcctac
      841 cgggtctggc cctatcgcat tctctaccgc cgtatcggct ctgagtggtt ggacgtgctc
      901 tccatcgaac accgcaaaga cgtctatcgg taggcggaat ggtccggcct gtccgtaacg
      961 aataaccaat ttgagtattc tgagtgacga ggcagtgaaa tgccgagctg gtggagcctg
     1021 gcaggtgctc cggcacctcc cggcgcgccc ctcccgggct cggccaggcg gaacgccttc
     1081 ggaaattccc tcctcgcccg cattattcat gagcgcttct gctgcaatcg ccgatctgct
     1141 gctacgacga gccttcggcc ggtgggctcg cccctcggac tctcaccgta ctgcacgagt
     1201 acgcatcgag tcctcgggac tccgcgcgcc ggtctcgcga cgcggctcag cgatttcgcg
     1261 acgaaccgtc atgaacaatg cgggctagac tcggtcagtt tccgtcttcc ttcctggcca
     1321 agcctcggtg ggtccccgcg cctccggtga cctccgctcc taccagtccc caccacctgc
     1381 aacatcagga aattgagaat gttcctgtct ctcccctgct tttacgtcat ccccttacgg
     1441 caccaaggtc aaggtaaaat cctggggtgt actcaccgtg gaaacgtccg ggatgttgat
     1501 cgactgaggt tggtaagcag ccgcagaggc ctggatcgta tagtgaactc caaccgctgc
     1561 agattgcggg gtaaacacga ttgggaggct tgtgctgtac aagccgagcg aaggcgtgcc
     1621 gatgggcagg gtcaagttgt agccgaaatc gccggtcgga tctaatggat tgacggttaa
     1681 gagggtagca ggcagcgatt tcacggtcac ggttggcccg ctgtcgagtg tttgcttcgc
     1741 tgcaacaaag acggttggtt cgtcagtggg tgggctcaat gttactgtgc cgctgatgct
     1801 atgggtcgcg gaggagctca aggcaattag cgaagtaggg gtatcgttgg tgctgaccgt
     1861 ggtaatgctg gtattggtcg gaacggggac tccgctaatc actgctgtcg catggctgtc
     1921 tgcggtgatc acaacatcgt aggttacccc tggagtaagg cgggccagga aaaatttccc
     1981 tctattgggg ttgggggctg ggtttgtgtt cggcactgtc gcgcgaacga tttcaccgtt
     2041 cacctgggcc gacacgacga cattgttgcc aagcaggctt gtatcgacga agccgtctat
     2101 gccgttcaag acaaatggga cgaccttaat cacgggtttg agtaggtact taccgttgcc
     2161 ggtccgcaca atagagcggc aggcatcaaa gtccagcacc agatcgaccc gctgtccgga
     2221 ccccacgtcg aactgatgga tcaacttgat tccgctttgg acggcactgg gcgttacgag
     2281 cggaatccta ttgccaatga ttccaggatc ttgaccttcc aggacaaccc aattggcagt
     2341 gggtgaatta ccattgttgt cgaccaagac cagacgcact tgcgtgtaat ggcctgccgc
     2401 taacggcgtc tcgccaagac tgtcaagcgc gagattgtgt tgcgttggat cattgaggtt
     2461 gagcaggttg attgtacgca cggggttgag agtgatatcg gtctagccgg cggccttatc
     2521 atctgccgag ttgctctgat gcacgcggac cttgctgaca gtgacattca cttcttcgaa
     2581 gccgcaggct ggtgcatcgg tcaacgacac accgagcaca ccggcttgtg ggctaccgcc
     2641 gtcgttaccg ctactaccgc atccggcgag caacagcgtg actactaatc ccagggacca
     2701 gatcagctgg ttagaacaac tcccgaacct cttcatatta ttagccccct gctttcttga
     2761 tacgtttgga gcccatctgt cttcagacca cccatgactc aacattctag aatgagcgag
     2821 ccagatcagg actatccccc gcagtctgag caaatatcat actgctcagt ctgtgggctg
     2881 caacggaatc tctacggcca acatgcagca tcgtgagtgc aatccacccc tggtacaaat
     2941 gcgcaataaa tcaaacgggt ctgagcatgt tccttttccg gcgaacactc ctgtgctgag
     3001 cttctcagga cgcattcctt tcgtgcaatt ccccattcgt aggttcagct ctgtaggcat
     3061 cctgatagag cggtggccac aagtatgtgg ggtggggtcg agcctctgag agactgcccg
     3121 cccataccat ggagagggaa cggaccccat ttcttcgaac gattgaggat aacagcaagc
     3181 aaggttacca tgggtaaacc gatcgcctaa cggaaaggtg cagaggtatg ggggtgaggc
     3241 agaagggtca ggcttggctg tctgtactct ggttgctgat ggctgtgacg ttggtcgctt
     3301 gcgtcgcagg actgccgcag acaccattac cggaaatcac aggcaaagac ggggcaccga
     3361 tgatgctggt tccaaggggg gagtttacca tggggagcaa tacaagcttt gcccatgaac
     3421 agcctttgca tcgggttacc cttgatgcct tttacatgga caagtacgaa gtgaccgtgg
     3481 ggcagtatac gaagtttttg gaggccacga gtttagctgc gccgccagac tggaacgtga
     3541 tgaatcagcc accccatcag aatcgaccag tcgtcaacgt caattggtgg gatgcgaatt
     3601 cctactgccg gtgggccggg aaacggctgc cgacggaagc ggaatgggag aaggcggcgc
     3661 gggg
//
LOCUS       DATMXI010000403         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_127499, whole genome shotgun sequence.
ACCESSION   DATMXI010000403 DATMXI010000000
VERSION     DATMXI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127499"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="gcvPB"
                     /locus_tag="VN444_07825"
     CDS             complement(<1..1237)
                     /gene="gcvPB"
                     /locus_tag="VN444_07825"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWR21749.1"
                     /translation="MKEGVRDTSPHPPFGKGGRGGISSGQSAKCEEEGAGVGAQGLTF
                     NEPLLFDQGSQGRRGCTLPECDVPELKAERLLPRKLLRHDISGFPELSEVEVVRHFTR
                     LSQWNYGVDTGFYPLGSCTMKYNPKINEEVWRLPGFSCAHPYQPESLVQGALELMYEL
                     EQFLAEVSGMDRVSLQPAAGAQGEILGMMLIRAYLESKGSPRKRVLVPDSAHGTNPAS
                     SAICGYEVLQIKSGPNGRLDPQVVANAMDEDVAAIMVTNPNTLGLFEDQIAEIAKIVH
                     AKGGQVYCDGANLNAIIGISRPGDTGIDVLHFNLHKTFAVPHGGGGPGAGPVGLKAHL
                     APFMPVPVIEKKGKRFVLNDDLPSSVGRVRAFYGNFAALVRAYAYIRSLGPSGLRRVA
                     EVAVINANYIMSQLKDHYHL"
     gene            complement(1234..2583)
                     /gene="gcvPA"
                     /locus_tag="VN444_07830"
     CDS             complement(1234..2583)
                     /gene="gcvPA"
                     /locus_tag="VN444_07830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWR21750.1"
                     /translation="MEYIPNTEADCRAMLDAIGVRSSEELFADIPQKLKLKRGLNLAP
                     PLSETGLRKHMKGLAGKNADVDHYSSFLGAGAYNHFIPAAVSHLVYRSEFYTAYTPYQ
                     PELSQGTLQAIYEYQTLICQLTGMEVANASMYDGSSALAEAILMAHRINGRREVVLPL
                     AVHPEYRTVSRTYVSKLGLHLHEVPYTDQGTTDLKKVKAALSDRTCAVVVQSPNFFGV
                     LESLDELAEAAHSAGALLIVVVAEPVSFGIVRSPGECGADIVVGEGQAFGNHLNFGGP
                     YLGFFASKEAYIRSMPGRLVGQTEDKAGRVGYVLTLSTREQHIRREKATSNICTNEGL
                     CALAATVHLSLLGRAGLRELALLNLRKAAYAKEAISKLRGYELSFAGPTFNEFVVRVK
                     RRTPAQVNRALLAKGIIGGLDLGRFYPELSDCLLLCVTEQNSREEIDALCKAMGGGR"
     gene            complement(2583..2969)
                     /gene="gcvH"
                     /locus_tag="VN444_07835"
     CDS             complement(2583..2969)
                     /gene="gcvH"
                     /locus_tag="VN444_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWR21751.1"
                     /translation="MIPEGLHYTKAHEWVKVEGDRGRIGITHFAQSQLGDIVFAEMPH
                     VGRVLRQMEAFGVVESVKAVSDLYCPLTGEVLEVNPSLESNPEQINADPYGQGWMIVA
                     RIADPKELGSLMTAEEYQAYLAAEDH"
BASE COUNT          597 a          993 c          904 g          624 t
ORIGIN      
        1 gcagatgata gtggtccttg agctggctca tgatgtagtt ggcattgatc acggccacct
       61 cggccacgcg gcgcaatccc gatggaccca gcgaacggat ataggcgtaa gcccggacca
      121 gcgccgcgaa gttcccgtag aaggcgcgta ccctgccgac acttgacggc aggtcgtcat
      181 tcaggacgaa tcgcttcccc ttcttctcga tgaccgggac cggcatgaat ggtgcgagat
      241 gcgccttgag acccaccggt cccgcacccg gaccgccgcc cccgtgcggg acagcaaacg
      301 tcttatgcag gttgaagtgc aggacgtcga tccccgtgtc gcctggcctc gagatcccga
      361 tgatggcatt caggttggcg ccgtcgcagt agacctgccc gcccttggcg tgaacgatct
      421 tggcgatctc ggcgatctgg tcctcgaata acccgagggt attgggattc gtaaccatga
      481 tggcggccac gtcctcgtcc attgcgttgg ccacgacctg tgggtccaag cggccgttcg
      541 gaccggactt gatctgcagt acctcgtagc cacagatggc ggaactcgcg ggattggtgc
      601 catgggcgga gtcagggacc aggacccgtt tccgtggact ccccttcgat tccaggtacg
      661 cccggatcag catcatgccc aggatctccc cctgggcgcc cgcggccggc tgcagtgaca
      721 cccggtccat cccgctcacc tcggccagga actgctccag ctcatacatc agctccagag
      781 cgccttgaac aagactctcc ggctggtacg ggtgcgcaca ggagaagcct ggcagccgcc
      841 acacctcctc attgatcttc gggttgtatt tcatcgtgca ggagcccaag gggtagaagc
      901 ccgtatcgac tccgtaattc cattgcgata gccgcgtgaa gtgccggacc acctcgactt
      961 cggacagttc ggggaaaccg gaaatgtcgt gccgaagaag cttccttggc agcagccgct
     1021 ccgctttcag ctccgggacg tcgcactcag gaagcgtaca gcctcgccgc ccctgagatc
     1081 cttgatcaaa caggagcggc tcgttgaagg taagtccctg tgcgccaaca cccgcccctt
     1141 cttcctcgca tttggcgctt tgcccagatg aaatcccccc tcgcccccct ttaccaaagg
     1201 ggggatgggg agatgtgtct cgcaccccct ctttcatcgc ccacctccca ttgccttgca
     1261 gagtgcgtcg atctcctcgc ggctattttg ctccgtcacg cagagaagta agcaatccga
     1321 gagctccggg tagaatcggc ccagatccaa gccgccgatg attcccttgg ccaacagcgc
     1381 gcgattcact tgagccgggg tccgcctttt cacccgcacc acaaactcat tgaaggtggg
     1441 acctgcgaag gaaagctcat agccgcggag cttggagatc gcttctttgg cataggctgc
     1501 cttgcggaga ttcagtagag ccagttccct gagcccggcc cgtcccaaca gggaaaggtg
     1561 aaccgtcgct gccagggcgc agagcccttc gtttgtacag atgttggacg tggccttttc
     1621 ccgcctgatg tgttgctctc gagtagagag cgtgaggacg tagcctaccc tcccggcctt
     1681 atcctcggtc tgtccgacca ggcgacccgg catgctgcgg atataggctt ctttcgaggc
     1741 gaagaagcca aggtaggggc caccgaagtt caggtggttc ccgaacgcct gaccctctcc
     1801 caccacgatg tcggctccgc actcgcctgg ggatcggacg ataccgaacg agaccggctc
     1861 ggccacaacc acgatcagga gggccccggc gctgtgagcg gcctctgcga gctcatctaa
     1921 cgactccagg acgccgaaga agttcgggct ctggaccaca acggcgcagg tacggtccga
     1981 cagcgccgcc ttcacttttt tcagatcggt cgtgccctgg tcggtgtagg gaacctcgtg
     2041 caggtggagg ccaagcttgc tcacgtaggt gcgggatacc gtccggtact ccggatgcac
     2101 ggccaggggc agcaccacct cccggcggcc gttgatcctg tgagccatca ggatcgcttc
     2161 ggccagggcg ctggaaccat catacatcga cgcgtttgcc acctccatgc ctgtgagctg
     2221 gcagatcagc gtctggtact cgtagatcgc ctgaagtgtc ccttgcgaca gctccggttg
     2281 atacggcgta taggcggtgt agaactccga cctgtacacc aggtgggaga cagcggcagg
     2341 gatgaagtga ttgtaggcgc ctgcccccag aaaggaggag tagtgatcca cgtctgcgtt
     2401 cttgccggcc aaccccttca tgtgctttcg cagtccggtc tcggacagcg gcggcgccag
     2461 gttcagcccc cgcttgagct tgagcttctg ggggatgtcg gcaaacagct cctcgctcga
     2521 ccggacgccg atggcatcca gcatcgcgcg gcagtcggcc tccgtgttcg ggatgtactc
     2581 catcagtggt cctccgccgc cagataggcc tggtactctt cggcggtcat gagactcccc
     2641 aactctttgg ggtcagcgat cctggccacg atcatccatc cctgtccgta agggtcggca
     2701 ttgatctgct ccggattcga ttcgagcgag gggttgacct cgagcacttc accggtcagc
     2761 ggacagtaca ggtcggagac cgccttcact gactccacga cgccgaacgc ctccatctga
     2821 cgcagtaccc gtccgacgtg tggcatctcc gcgaatacga tgtcgccgag ttggctctgg
     2881 gcaaagtggg tgatcccgat acgtccccga tccccctcaa ccttgaccca ttcatgcgct
     2941 ttcgtataat gcaacccttc agggatcatg agcacctcca acggcggttc agagttgata
     3001 gttcatggtt catcgtcagt agcagaagaa caaacaacca agtaatccaa agcgatgcgt
     3061 gagaaaagcg acaatgacct ctgcatcagc tttccttcgc ggcgagatat ctatgaac
//
LOCUS       DATMXI010000404         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_52808, whole genome shotgun sequence.
ACCESSION   DATMXI010000404 DATMXI010000000
VERSION     DATMXI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52808"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VN444_07840"
     CDS             <1..202
                     /locus_tag="VN444_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HWR21752.1"
                     /translation="DRADYNWAREIIVQYDLATRSRVLFSPVFSKLDVRELAEWILAD
                     KLPVRFQIQLHKYIWAPDMRGV"
     gene            288..1790
                     /locus_tag="VN444_07845"
     CDS             288..1790
                     /locus_tag="VN444_07845"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWR21753.1"
                     /translation="MQVTVQVERAETAAAEALVLTHCEGEGLAKQDAAPLDRALGSSL
                     SKLMQSKEFEGKANDVLLYHTHNKVPAKRLVLAGLGKKKEVTLEKIRQAMGSAVKRVR
                     QAKVGAYSVLLPTVAPPGMSWVEVAQAMVEGAILGSYQFTVYRSEVASGQGVAEMRII
                     IPNKGLLRQVTEGIRRGVATAEATAFVRDLCNHPSNVLTPAKVAEEAKSIAKAEKITI
                     KILEQNEMERLGMGALLGVARGSQEPPKFIILEYNGSKKKDERPVVLVGKTITFDTGG
                     ISLKPAENMEHMKADMTGGAEVLASIRAAARLKLPLRLISILPVAENMPGGRAMKPGD
                     IVRTLSGKTVEVQNTDAEGRLILADGLAYAMRYKPAALIDIATLTGACVVALGQFAIG
                     MFGTDQKLKEQIRKSGQKAGERVWEMPLWEEYFEQLKSDVADMRNIGGRGGGMITAAL
                     FLSKFVGDCPWVHIDIASTDWSERERAYIPKGPTGIGTRLLVQYLIDRSI"
     gene            complement(1812..>2094)
                     /locus_tag="VN444_07850"
     CDS             complement(1812..>2094)
                     /locus_tag="VN444_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21754.1"
                     /translation="DAEKAHPLHSRFIEIPNVRMRFSEIGSTGGAFPFAKTHCKGERP
                     HEVRVGTSSGFDSPAALPDGLSEQPAKAVGLTLLDIFRSCFDRLSTNGV"
BASE COUNT          524 a          539 c          642 g          389 t
ORIGIN      
        1 cgaccgagct gactacaact gggcacgcga gattattgtt cagtatgatt tagccacccg
       61 tagtagggtc ctctttagtc cggttttctc caagctcgat gtgcgtgagc tggcagagtg
      121 gatcctggcc gataagctcc cggtgcgatt ccagatacag ttgcataaat atatctgggc
      181 acccgacatg cgaggggtgt gaattgtgag cgagaaaggc aacatgtgcg cgacaggcgc
      241 gactcgcaaa taacgagcga cgaaaacgaa ggagcagtgt gaagatcatg caagtcacag
      301 tacaggttga gcgagcggaa accgcagctg ccgaggcact ggtactcact cactgtgaag
      361 gagagggatt ggccaaacag gatgcagccc cgcttgaccg ggccctcgga agttcgttga
      421 gcaagctgat gcaatccaaa gaatttgaag gcaaggccaa cgacgtcttg ctgtatcaca
      481 cgcacaacaa agtaccggcg aaacggttgg tgctggccgg gctgggcaag aaaaaggaag
      541 tcacgctaga aaagatccgg caagcaatgg gttcagccgt gaagcgcgtc cgccaggcga
      601 aggtcggagc ctattcggtg ctactaccga cggtcgcacc ccccggaatg tcatgggtcg
      661 aggtggcgca agcgatggtt gaaggggcga tcctcggcag ttaccaattc actgtctatc
      721 gaagcgaggt tgcatccggg caaggcgtgg cggagatgag gattatcatc ccaaacaagg
      781 ggttgttgcg gcaggtgacc gagggaatca ggcgcggcgt cgccacagca gaagccaccg
      841 cctttgtccg ggacctttgt aatcatccct ccaacgtcct gacccccgcg aaagtcgccg
      901 aagaagcaaa atccattgcc aaggctgaga aaatcacgat caaaattctc gagcagaacg
      961 agatggagcg cttggggatg ggtgcgctgc tcggcgtagc gcgcgggagc caggaaccgc
     1021 ctaagttcat catccttgaa tacaacggat ccaagaaaaa agatgagcgc ccggtggtgc
     1081 tcgtcgggaa aacaatcacc ttcgatacag gtgggatttc gctgaaacct gccgagaata
     1141 tggaacatat gaaggccgat atgacgggag gggccgaggt cctggcttcg atccgtgcgg
     1201 cagcccgact caagcttccg ctgcgcctca tcagtatcct gcccgtagcg gaaaacatgc
     1261 ctggtggccg agccatgaag ccgggcgata tcgtaaggac gctgtcggga aagaccgttg
     1321 aagtacagaa cacagatgca gaaggccggc tcatcctggc cgacggcctc gcctatgcaa
     1381 tgcggtataa gcctgcagcc ctgatcgaca ttgcaacgct cacaggagcc tgcgtcgtag
     1441 cgctcggcca gttcgcgatt ggaatgtttg ggactgatca gaagctcaaa gagcagatca
     1501 gaaagtcggg acaaaaggcc ggtgaacggg tgtgggagat gcccttgtgg gaggaatatt
     1561 tcgaacaact caagagcgat gtcgccgata tgcgcaacat cggtggtcgt ggcggcggca
     1621 tgatcaccgc tgcgctcttt ctgagcaagt tcgtggggga ctgcccatgg gtacacatcg
     1681 atatcgccag cacggattgg agtgagcggg aacgggctta cattccaaag ggccccacag
     1741 ggatcggcac caggttactg gtgcaatacc tgatcgatcg aagtatctag tgcaaagcgc
     1801 cagaaatagc cttacacacc gttcgtgctg agcctgtcga agcacgaacg gaatatatca
     1861 agaagcgtta gacccactgc cttagcaggc tgttcagaaa ggccgtccgg caaggccgca
     1921 ggcgagtcga acccggagga ggtaccgacc cgcacttcgt ggggccgttc gcctttacaa
     1981 tgggtcttgg cgaacggaaa agcccctcca gtacttccga tctcggagaa tctcatccgt
     2041 acgttgggga tttcaatgaa ccgagaatga agcgggtggg ctttttcagc atcc
//
LOCUS       DATMXI010000405         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_121150, whole genome shotgun sequence.
ACCESSION   DATMXI010000405 DATMXI010000000
VERSION     DATMXI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121150"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..846
                     /locus_tag="VN444_07855"
     CDS             184..846
                     /locus_tag="VN444_07855"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HWR21755.1"
                     /translation="MLVSFAIKQTEHARSVLKLGTSPDTMLIARSMLEGLSQLLWAMK
                     QPTRRPLLWRAFAFVLDWRLLQRHRAEGRTIDSDVERHTHAGLRRHGRWFLTKEARQA
                     RANGRLLPLDPYVKNWYGEREVEIFRDVGGQVLLEHAYGPFSEWHHWRIGAIGRLVSF
                     DAGAREFTMTTFRPSSVALAVACAFQCLWQTMWLMNARCHLRIGRQLRHLHHQQVSIP
                     RP"
     gene            1041..1274
                     /locus_tag="VN444_07860"
     CDS             1041..1274
                     /locus_tag="VN444_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HWR21756.1"
                     /translation="MEKFSRGAGPEGNISLAMVAQQQQQLQAIEVAIQRHKAGTYGRC
                     IGCGEEIPLPRLQALPFAQRCAACQEEWEAEKR"
     gene            1291..1752
                     /locus_tag="VN444_07865"
     CDS             1291..1752
                     /locus_tag="VN444_07865"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWR21757.1"
                     /translation="MDRLYRLSQEERNAVRNQIAAELSHEPDVLFAYVYGSFLKSEAF
                     HNIEIGVYLSSSHFNNAMTANLSAHLSAKVTLPVDVRTLNTAPISFRLYVLRGECLFS
                     RDDNLRTGIMEDTVQRYLHNASPRHNTTKEALGYGESHDPNADSTRTDADG"
     gene            complement(1909..2307)
                     /locus_tag="VN444_07870"
     CDS             complement(1909..2307)
                     /locus_tag="VN444_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HWR21758.1"
                     /translation="MDRLFLDANVLFSAAYRPDAGLRRLWQIVSVELITSAYAEEEAR
                     RNLETPAQHATLDALLRHVKVLIFQPESSHLPSGLDLPDNDRPILEAAIFSQATHLLT
                     GDMKAFGAYYGRTIAGVLILPPAAYLRGHR"
     gene            complement(2311..2607)
                     /locus_tag="VN444_07875"
     CDS             complement(2311..2607)
                     /locus_tag="VN444_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR21759.1"
                     /translation="MIAETSRVGKRGTIVIPAAIRRRLKIEEGAFVIVEEREDGVLIR
                     PAAVMPIEIYTAQRRAEFLLSNAVDAEDYARAREEVRRMGLDPDAIPHYAVAGD"
     gene            complement(2778..>2979)
                     /locus_tag="VN444_07880"
     CDS             complement(2778..>2979)
                     /locus_tag="VN444_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21760.1"
                     /translation="LPSSRKVRLLGVTASRLSDLRGPGRQLSLELDPAGAKQRRLTEA
                     VDRIQARFGKGAIRPASLLSSS"
BASE COUNT          652 a          895 c          845 g          587 t
ORIGIN      
        1 ggcgctcaca ctcatcactg gctgcgttca tgaattttgc aggctaagaa cactaagaga
       61 atgacagctg acgaccgagc acgcaaaaat cttcctgttt tgcgagagaa cctcagcgcg
      121 ctcctccatc ttgcaccaaa gggaatgcgc gtcaacatcg gcacccgcga tcacctgcga
      181 tttatgttag tgtctttcgc aatcaagcag accgagcacg caagatcggt gctaaagctc
      241 ggcaccagcc ccgacacaat gctcatcgcc agaagcatgc ttgaagggct ctctcaattg
      301 ctctgggcga tgaaacagcc tacgcgtcga ccacttttgt ggcgcgcgtt cgccttcgtc
      361 ctcgattggc gattgctcca acgccatcgg gccgagggac gcaccattga ttcggacgtt
      421 gaacgccata cccatgcagg gctgcgtcgc catggtcgct ggtttctaac aaaagaagct
      481 cggcaggcga gagcgaatgg ccgtctcctt ccgctagatc cgtatgtgaa gaattggtac
      541 ggtgagcgcg aggttgaaat ctttcgcgac gtgggtggcc aagtgctatt agaacatgcg
      601 tacggcccgt tctcggagtg gcaccactgg cgcatcggcg cgatcggacg ccttgtctcc
      661 ttcgacgcgg gagcgaggga gttcacaatg acgacattcc gtcccagtag tgtagcccta
      721 gccgttgctt gtgctttcca gtgtctctgg cagacgatgt ggctgatgaa cgctcgatgc
      781 cacctcagga tcggccgaca gctccgacac ctccaccatc agcaggtcag cataccaagg
      841 ccatgaggcg cgccgcgtcc gaccgcgaca gtctaactcg ctgctccagg cgacggccaa
      901 gaacgggccg cgccaccgct gagccggagc gtgtgggatt caagggacag tttactcaat
      961 tcataggttc gagcttccgg aattcaatat ggtgtcctca gaatcgataa acctcacgct
     1021 ttcagagggt gggaaaggtg gtggagaagt tctcgcgagg cgcggggcca gagggcaaca
     1081 tcagcctggc catggtggcg cagcaacagc aacagcttca ggcgatcgag gtagccatcc
     1141 agcgccacaa ggccgggact tatggtcgct gcatcggctg cggcgaggag atcccccttc
     1201 cccgccttca ggccctgccc ttcgcccagc gttgcgctgc ctgccaggaa gagtgggaag
     1261 cagaaaagag atgacgcggg gacaagcctg atggaccggc tctaccgact gagccaggag
     1321 gaacgcaacg cggttcggaa tcagatcgcc gctgaactct cacatgagcc cgatgtgctt
     1381 ttcgcctacg tctacggctc atttctcaaa tctgaagcat tccacaacat agagatcggc
     1441 gtctacctct ccagcagcca cttcaacaac gccatgactg cgaatctgtc ggcccatctg
     1501 agcgccaagg tcacacttcc agtcgatgtc cgcaccctca atacggcacc gatttccttc
     1561 cggctctacg tcttgcgtgg tgagtgtctg ttcagtcgcg acgacaacct ccgcaccggt
     1621 atcatggagg acaccgtgca gcgctattta cacaacgcat ccccgcgtca caacaccaca
     1681 aaggaagccc tcggctatgg cgaatctcac gacccgaacg cggactctac gagaacggac
     1741 gccgacggct gatacatcgc gctgttgagc ctaccctgca aacccccgtt cacccttcga
     1801 cacgttcagg gcaaacggca tgggcattga caacctagac ttattccgtt cgtgctgagc
     1861 ctgtcgaagc acaggaaggg atttagaaca ggctctatcg gtatcggatt atcgatgccc
     1921 acggagatac gcagcgggcg gcaggatgag aacccctgcg atcgtacgac cgtaatacgc
     1981 gccaaaggct ttcatgtcgc cagtgagcag atgagtggct tgactgaaaa tagcagcttc
     2041 caagatcggt cggtcgttgt ccggaagatc gagaccggaa ggtagatgag aggactcggg
     2101 ttggaagatc agcaccttga catgacgaag cagcgcgtcc agggtagcgt gctgagcagg
     2161 ggtctccagg ttgcgacggg cctcttcctc cgcgtatgct gacgtaatga gctccacact
     2221 aacaatctgc cacaagcgcc ggaggccggc atcgggtcga taggctgcgg aaaacaggac
     2281 attggcatcc aggaagagcc ggtccacctg tcaatccccg gctaccgcat agtgggggat
     2341 agcatcaggg tctagcccca tgcgcctgac ctcctcgcgc gccctcgcat agtcctcagc
     2401 gtcaaccgca ttcgagagaa ggaactctgc gcggcgttgg gccgtgtaga tctctatcgg
     2461 catgacggca gcgggacgga tcaggactcc gtcctctcgt tcttcgacaa tgacgaatgc
     2521 gccctcttcg attttgaggc gacgacggat cgccgccgga atgacgatag ttccccgctt
     2581 tcccaccctg ctcgtctctg ctatcatgag ctctcctata cctgattatc tgatactctg
     2641 atgatctgac tataggatag actgagacaa gagtcttgtc aagatggcac ggggtactca
     2701 cctgaaggcg attcaggagc ttgttggcaa cctatacttt tcccggctga agctatgggt
     2761 gttgcagaga tgcgtggtca actgctggag agcaggctgg ctggacggat cgcccccttc
     2821 ccgaaacgcg cctggatacg atccacggcc tccgtcaacc ggcgctgctt cgcgccggca
     2881 ggatccagct ccaacgagag ttgccgcccc ggtcccctga gatcggacag cctcgaggcc
     2941 gtcaccccca ggagacgcac cttgcggctc gaggggagc
//
LOCUS       DATMXI010000406         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_96188, whole genome shotgun sequence.
ACCESSION   DATMXI010000406 DATMXI010000000
VERSION     DATMXI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96188"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..919
                     /locus_tag="VN444_07885"
     CDS             437..919
                     /locus_tag="VN444_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWR21761.1"
                     /translation="MLRGRRYWLMKSEPRVFSIDDLAQSPRRTTCWDGVRNYQARNFM
                     RDMAVGDQVLFYHSNAEPPAVVGIAEIVRTAYQDETQFDKTSHHYDPASTASAPRWDM
                     VDIRYRRTFKKSLSLDRLRQEPKLKNMVLLRKGSRLSVQPVTEAEWMLVLKLAGVEAD
                     "
     gene            complement(957..1298)
                     /locus_tag="VN444_07890"
     CDS             complement(957..1298)
                     /locus_tag="VN444_07890"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWR21762.1"
                     /translation="MAIEKDYLLPGSRTVYVDPDTTRREGNLVRIWQLTDFTWMQGSA
                     RGPARFLSTTTHKQLDCREKLVRLLAYREFSLQMGTGIRVDGYVDKDHWLPVEPQSIN
                     YALWELACGQE"
     gene            1745..2227
                     /locus_tag="VN444_07895"
     CDS             1745..2227
                     /locus_tag="VN444_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWR21763.1"
                     /translation="MSIAPTLNAFLDHEHVHYDLLSHPQAFRAAEIAHTLHTPEKEMA
                     KVVIVKVDERFVMTVLPASWNVDLHRLRAFFPARHVRLASESEIAGLFPDCEVGAMPP
                     FGTLYGLPVYVDRSLAEDKEIVFQAGTHSDAIRMRYMDFSALVFPVVAEFHRSPPNVC
                     "
     gene            2294..>2629
                     /locus_tag="VN444_07900"
     CDS             2294..>2629
                     /locus_tag="VN444_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR21764.1"
                     /translation="MAVDGSEHYARALHYLGTLLRETRDVQVTLFHVLKPMPRELPEH
                     GGSEDPAEEVRLAKKLQEDQENWVRTESRLEHPILLTALELFGKTGFPLDRVTLKFGH
                     EDDVAHTILD"
BASE COUNT          610 a          693 c          696 g          630 t
ORIGIN      
        1 ttcgcagatg cctcggaggg caatcccatc aatccggcca tcgcaaggac catggcccct
       61 gcaacggcca tcagttgagg tgattgtctt ttcatcatag ttaaactccc ttctgaaatg
      121 gttgggcgaa cttctgcagg caaatgtgca actggtgttt tctatgtgag ggtctcacag
      181 gcacttcgtc tgtgtgtgag aaagagaata acacagcaaa actctttggt gtatatcgta
      241 gtgtccggtt aagagtcaag ccctttgtta atcccctgta gatagcaagc taagtagacg
      301 ctggttgtgc agtcagcagg agggagaggg gttgcctctc cctcccttgc ttttggcaaa
      361 gagatgattt atacgctcgg tgcttggtct caatacgcta cacaatttgt tgccatacgc
      421 gatgcaaggt aaagagatgc tacgaggacg acgctattgg ctgatgaaat cggaaccacg
      481 agtcttttcg atcgatgatc ttgcacaatc cccgagacgg actacctgtt gggatggtgt
      541 acggaactac caggcgcgca actttatgcg tgacatggct gtcggcgacc aagtgctttt
      601 ttaccatagt aacgccgagc cgcccgctgt cgtggggatt gcagagatcg tgcggactgc
      661 ctatcaggac gaaacgcagt ttgacaagac cagccaccac tacgacccag ccagtactgc
      721 ctctgctcca cggtgggaca tggtcgatat ccggtatcgc cggacattca agaagagtct
      781 gtcactcgac cggttgaggc aagaaccgaa gttgaagaac atggtcttat tgagaaaagg
      841 ttcccgtctc tcagttcaac cggtgacaga agccgaatgg atgctggtgc tcaagctggc
      901 tggtgtcgag gctgactaag cgggaatctc ttcgctatat caccgcggat gtacgatcac
      961 tcctgaccac aggcgagttc ccacagagcg taattgatac tttgcggttc aaccggcagc
     1021 cagtggtcct tgtctacata tccatcaacc cggatgccgg ttcccatctg gagcgaaaac
     1081 tccctatacg cgagcaatcg aacaagcttt tcgcggcagt cgagttgttt gtgagtcgtt
     1141 gtcgataaga atctggcagg gcctctcgca cttccttgca tccatgtaaa gtcagttaac
     1201 tgccagatcc tcacgaggtt cccttctcgt cgagtcgtgt ctgggtcgac atagacagtc
     1261 cgcgatcctg gcaacagata gtctttctca atcgccaccc actttgcatg agctggaaca
     1321 ttgaacaaaa acaagactgt gatcagggac cagaaacccg cgaggtaggc tgacggcgga
     1381 ttcaagacat agcgcacttg gtgctggcaa gacatcacag gagaaatcgg acaagtggag
     1441 tgagctgtgg caattgcttc agttcgtctt gcgtgcactc cgcaagggct atactgctcc
     1501 attccatcgc tttagaagag aggatgtcaa ggatcggcta tgtgtgaatg tgaagctttc
     1561 ttgagcaaga caaacggcgt atactttagc agatcgagtg cggtcatcat gatcagagcg
     1621 gagtcttgat cgtgaatcac cctgccccct ctttttcaaa tcgtcatcga acaatcctgc
     1681 gtcccccttt aaccattccg gccatgacct ccgtccagag gtcgcccaca tgaggaggtg
     1741 ttagatgtct attgcgccga cacttaacgc atttctcgat catgaacatg tccattatga
     1801 cctgctgtca catccccaag cgtttcgcgc cgcggagatc gcccacacgc ttcacactcc
     1861 agaaaaagaa atggccaagg ttgtgattgt gaaggtagat gagcggttcg tcatgacagt
     1921 actgccggca agctggaacg tggatctcca ccgccttaga gctttctttc cagcccgaca
     1981 tgtgcggcta gcaagcgaga gtgagatcgc gggcctgttt cctgactgcg aggttggcgc
     2041 gatgccgcca ttcggcaccc tctatgggct tcctgtatat gtcgaccgat ccctcgcgga
     2101 ggacaaggag atcgtgtttc aagccggaac ccactctgat gcgattcgca tgcgctatat
     2161 ggatttttcc gcactggtgt ttcctgtggt tgcggaattc caccgttcac cccctaacgt
     2221 ctgttgactg gtcatgtcct aaccgcttcc gacgacaacc catcccggac aacggctcct
     2281 ctcaaggatc ttgatggccg tcgatgggtc cgagcactac gcccgggccc tgcactatct
     2341 gggaacgctc ctgcgtgaga ctcgtgacgt gcaggttaca ttgtttcacg tgctgaaacc
     2401 gatgccgcgc gaacttccgg aacatggcgg atctgaagat cccgcagaag aagttcgtct
     2461 tgccaagaag ttacaggaag accaggagaa ctgggtccgc acggaaagcc ggcttgaaca
     2521 cccgatctta ttgacagcgc tggagctgtt tggaaagacg ggatttcctc tggatcgcgt
     2581 cacgctgaag tttggccacg aagatgatgt tgctcatacg attcttgac
//
LOCUS       DATMXI010000407         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_121144, whole genome shotgun sequence.
ACCESSION   DATMXI010000407 DATMXI010000000
VERSION     DATMXI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121144"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VN444_07905"
     CDS             complement(<1..1209)
                     /locus_tag="VN444_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR21765.1"
                     /translation="MMRPATAHPARRSISPPSTHEVDQFVDELCREYSTRGHPVHLRQ
                     IPSQSPSYANLSDPLPEPLRDTLRKIGVEQLYTHQCAAIEAARAGKHPLVVTATASGK
                     SLAYLLPILEYLLTDRSARALLLFPIKALEQDQLKMLQTLLPPGHGINAAILDGDTPA
                     SRRAKLRDNPPQLLLSNPDMLHLSLLPYHAQWREFWRNLRYVVLDELHTYRGVFGSHI
                     AHVLRRLRRVAAAYGAQPQFIACSATIANPLGLSEQLTGLPFHLIDGDGAPQQGKRFL
                     LINPISSPYTEATDLLLRCLRKGLKTIAFAKARKIAELIAMWTRQADPTLGSRLAAYR
                     AGFTADERRTIERGLFTEQLAGVVTTSALELGIDVGGLDACLLVGYPGSITSTWQRGG
                     RVGRGRREALI"
     gene            1597..1974
                     /locus_tag="VN444_07910"
     CDS             1597..1974
                     /locus_tag="VN444_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR21766.1"
                     /translation="MTASLKIIKTKVGLLELAKQPGNVSQACRIMGYSRDSFYRFKNL
                     YATGGEAALQEISRQRPILKNRVAFRKTIYRTIEELQVNLDAWLQEYNERRPHQGRWY
                     YGKTPMQTFLDSVPLAKEKLLAA"
     gene            2354..>3232
                     /locus_tag="VN444_07915"
     CDS             2354..>3232
                     /locus_tag="VN444_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21767.1"
                     /translation="MNGVLLGEETAPRENWGDYPNAGIDLRGATQLTFWARGELGGER
                     VEFFVFGVGWSVETGQPVMPYPDSSKKLSPGVVTLSSQWRRYKLKLKGKDLSYVLGGF
                     GWVASAEQNGDRDITFYLDDIKYNKPRLGDPRFLVSYQTTCSGSPFDQVMRNVAFTYD
                     NAVTLLAFLASRDVKRARLLADALVYAQEYDRFYSDGRVRNAYQGGDLILFPGWTPHG
                     KGGTVRMPGWYDTVAQQWYEDRFQVSTHTGNVAWAMLALLKFYQATEDGKYLTAAKRM
                     GEWIETNCRDSRGAGGY"
BASE COUNT          693 a          872 c         1016 g          651 t
ORIGIN      
        1 gatcagcgcc tcccgtcgac cgcggcccac ccgaccgccc cgttgccagg tgctcgtgat
       61 gctgccggga tagccgacga gcaggcaggc atccagtccg ccgacgtcaa tcccaagctc
      121 caaggctgag gtggtgacaa cccctgccaa ctgttcggtg aacagcccac gttcgatcgt
      181 ccggcgttcg tcggccgtaa agcctgctcg atacgcggca agtctgctgc cgagtgtcgg
      241 atcggcctgc cgtgtccaca tcgcgatcag ttcggcgatc ttccgagcct tggcgaaggc
      301 gatggtcttt agcccttttc ggagacagcg gagcagcagg tcggtcgcct cggtataggg
      361 gctgctgatc ggattgatta gaaggaagcg cttgccctgc tgtggggccc catcgccatc
      421 gatcaggtga aatgggaggc ccgtgagttg ctccgacagc ccgaggggat tcgcgatcgt
      481 tgccgagcac gcgataaact gcggctgcgc tccgtaggcg gccgctaccc gccgcagacg
      541 ccgcaggacg tgggcgatgt gcgagccgaa gaccccgcga taggtgtgca gttcgtccag
      601 cacgacatag cgaaggttgc gccagaactc gcgccactgg gcgtggtagg gcagcagcga
      661 caggtggagc atatccgggt tgctcagcag cagttgtggt ggattgtccc ggagcttcgc
      721 tcgccgggat gcaggcgtgt ccccgtccag tatggcggcg ttgatgccgt gaccaggagg
      781 aagcagcgtc tgtaacatct tcagttgatc ctgttccagc gccttgatcg ggaataacag
      841 cagggccctc gccgaacgat cggtcagcag gtattccagg atcggcagca gataggcgag
      901 gctcttaccc gatgcggtgg cggtgacgac taatggatgc ttgccggcgc gggccgcctc
      961 gatagctgcg cactgatgcg tgtagagctg ctcgactccg atcttccgta gggtgtctcg
     1021 aagcggttca ggcagaggat cggacaggtt cgcataactt ggcgattgtg aagggatctg
     1081 tctgagatgg acggggtgtc cgcgagtcga gtactctcga cacaactcgt cgacgaactg
     1141 atcaacctcg tgggtggatg gaggcgagat ggaacgccta gccggatgtg ccgtcgcagg
     1201 tcgcatcatg ctcaggcgct ttgaaccaca accctcatac catctactgt ttttcgaagc
     1261 cacatatgcg ccacagcttt agcacggtgt gtgaaagcgt gtcaaggcca aaaccggttt
     1321 agacctgact ttctcattga caccggcgtt agtggagcgc atattgattt agaaagaggt
     1381 tacccttctg ctgtcgagag cgaggacacg ccgatggtgc ccagtcggga cccgcaatcg
     1441 aagtggcgca atgcgcgtga acggcccttt gggggcatga gccccagagg gccgttgtgt
     1501 tgaaggccat accccgttgt ttgtaaatac tagaacttaa tctgacagtc aggtagaatc
     1561 ctcgttagcc cctgaggggc gaaaggagcg aggagcatga ccgcgagcct gaagatcatc
     1621 aagacgaagg taggactctt ggagctggcc aagcaaccag gcaatgtctc gcaagcctgc
     1681 cgcatcatgg gctatagccg tgacagcttc tatcgcttca agaatctcta cgcgaccggc
     1741 ggcgaggcgg cgctccagga gatctcccgc cagaggccga tcctgaagaa ccgggtggcc
     1801 ttccggaaaa cgatctatcg cacgattgag gagctacagg tcaatctgga tgcctggctg
     1861 caggagtaca acgagcgtcg accccatcag ggccgctggt actatggcaa gaccccgatg
     1921 cagacgtttc tggatagcgt gccgttggcc aaggagaaac tattggcggc gtaacccggt
     1981 gagaattccc tgtgtgtcag atgaagtctt gacatttaca tgaatgaaac gggtaatggg
     2041 caaacctaaa ctaagactgg ttacgctggc actgttctgc cttatttggg ctcctagcct
     2101 cgcgcaggcg cagaaatctc cagtcactta tctgcgcggg gttctcgatc agcaccataa
     2161 gactctcgat gtgtataccg acgctcacgc ggccggaaat catttcgtgg cccgtggaag
     2221 gatgagcagc tccggagacg aagatgccgt tccgaccatg caggaagcct ggacgggctc
     2281 gccgtattcc cgatgcacgt gcatcaaagc tagcttcaaa tcgaaaggtt cgaactgggg
     2341 cgggtggtac ttcatgaacg gcgttctcct gggagaagaa acagcaccga gggagaactg
     2401 gggggattac ccgaacgccg ggatagactt gcgtggcgcc acgcaactga ccttttgggc
     2461 gcggggggag cttggcggag aaagggtaga gttcttcgtt ttcggtgttg ggtggagcgt
     2521 cgagaccggt caacccgtga tgccttaccc cgactcctcc aagaaactct ctccgggtgt
     2581 tgtgaccctc tcgtcccagt ggaggcgata caagttgaag ctcaaaggca aggacctgag
     2641 ctatgtattg gggggcttcg gatgggtggc gagcgctgag cagaacggcg atcgcgatat
     2701 caccttctac ctcgatgaca tcaaatacaa caagccgaga ctcggagacc cccggtttct
     2761 agtgagctat cagacgacct gttccggctc tcccttcgac caggtgatgc gaaacgtggc
     2821 ctttacctac gataacgccg tcacccttct ggcgtttctg gcttcgaggg atgtgaaacg
     2881 ggcaaggctt ttggctgatg ccttagtata tgcccaggag tatgaccgat tttactcaga
     2941 cgggagggtc aggaacgctt accaaggtgg agatctgatc ctctttccgg gctggacacc
     3001 ccatgggaaa gggggaacag ttcgaatgcc gggctggtat gacacggtgg cgcaacagtg
     3061 gtatgaggac cgattccaag tgagcaccca tactggaaat gtagcgtggg ctatgcttgc
     3121 ccttctaaag ttctaccagg caaccgaaga tgggaagtat ctcaccgcag ccaagagaat
     3181 gggcgagtgg attgagacta actgccgaga cagccgagga gcgggcgggt ac
//
LOCUS       DATMXI010000408         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_8970, whole genome shotgun sequence.
ACCESSION   DATMXI010000408 DATMXI010000000
VERSION     DATMXI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8970"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..386)
                     /locus_tag="VN444_07920"
     CDS             complement(54..386)
                     /locus_tag="VN444_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR21768.1"
                     /translation="MNWAITYYSESLQQEILAMPAGFLGRYLRYSDRMEAFGPDLGMP
                     HTRAMGEGLFELRLKAEEGMARVFYCTMGGKKIVMLHQFRKKTDKTPLRELETARRRM
                     REIKNAHT"
     gene            791..1585
                     /gene="thiD"
                     /locus_tag="VN444_07925"
     CDS             791..1585
                     /gene="thiD"
                     /locus_tag="VN444_07925"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWR21769.1"
                     /translation="MRTALTIAGSDSGGGAGIQADLKTFAALGVFGTSAITSVTAQNT
                     VSVQGVHDLPPEFVGRQIDSVLEDIAIDAAKTGMLSSAAIIEVVAAKVKAHAIERLVV
                     DPVMVAKGGAPLLQPDAVNALIEHLLPLALMVTPNVPEAEALTGLTITELNEMRQAAR
                     QIHGLGPRYVVLKGGHLGSRAVDLLFDGSRFTELDAERIDTPHTHGTGCVFSAAIAAE
                     LAKGSPVPEAVATAKRFITAAIRHGFRLGKGFGPTDPIAAARSLIP"
     gene            1621..2304
                     /gene="nfi"
                     /locus_tag="VN444_07930"
     CDS             1621..2304
                     /gene="nfi"
                     /locus_tag="VN444_07930"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132499.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HWR21770.1"
                     /translation="MNYIPRTLHRWDVSPQEAMAIQLKLRSQLRLHGSGPFATVAGVD
                     VAYDETLKLMFAGVVVMSGDGREVLDVATAAASVNFPYIPGLLSFREMPAVIKAWNGL
                     KTMPDCLICDGHGLAHPRRFGLACHLGLLLDLPSIGCAKSRLVGTHQEPRKRRGSVAP
                     LLDQGEQIGVVLRTKDGVTPVFVSQGDRISLDTAVQTVLATCRGYRLPEPQRRAHLLV
                     TKMRLTTRA"
     gene            2453..3223
                     /locus_tag="VN444_07935"
     CDS             2453..3223
                     /locus_tag="VN444_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWR21771.1"
                     /translation="MELGLRGKVVMVTGGSRGIGRAIALGCAEEGCRLSICGRGLPAL
                     EEVAETIKASGVELLTVPADLTDPHGAERFVRATLDRYGRIDVLVNNVGGSRRGELEV
                     LLEQDWREVYDLNFFSTLRMARLVVPVMKRQGGGRIINIASIWGRESGGAMTYNASKA
                     AVISLSKSLAKELAPHNILVNSVAPGSTLFPGGSWDRRQRENPAQIAQFIKDELPLGR
                     FGRPEEVAAVVVFLASQQASLVTGACVTVDGCQSRSLI"
     gene            3843..>5639
                     /locus_tag="VN444_07940"
     CDS             3843..>5639
                     /locus_tag="VN444_07940"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HWR21772.1"
                     /translation="MSARSGRLWRARCLWGLSLGLVLSGGVMVHALTPSDLAGSTLNA
                     SVHVGRPVAHAGDNFNAATGTPTTLDGSASFDQDARLLTFRWRLQHAPRGSAATLVDS
                     TQPNPTFTPDVPGRYKFELVVTNGSRDSSPAYVKMTAFASGTAPPNARPGRDQSGFVG
                     TPVALDGGESDDPEDALLLFVWSFRRVPPHSSLTSADIVGRDTAMPSFIPDVRGEYRL
                     LFQVSDGVLTDEAIVLVVADSLVGPHADAGPDQLVEGLGPITLDGRDSNDSDGRPSPV
                     SYSWRLVARPPGSALTTDALQHATTVTPTFTPDVLGAYIFRLQVSDGSSTDEDNVLIE
                     VASQVNHPPIAQDDAAVTDENAPVGITVLGNDMDPDSDPLTITDVTQGANGGTVAING
                     VTATYTPPANWSGTDSFTYSISDGRGGTDTGSVTVTVNLVNDPPTVTLGAEQTSGPLP
                     LVVAFTAATSDSDGDLLTYAWDFGDGTTKPTGSAIESHTYQTAGSFTATVTVSDGQAT
                     AQASLDITPSGVPDPSTVAPPLSQTVTTDLGSGTAFLYSGSNPIQTGVAPGTINLTRA
                     AVLRGRVLNSLGTPLPGVAIGILNHPELGQTQS"
BASE COUNT         1075 a         1694 c         1733 g         1137 t
ORIGIN      
        1 gtgcatattc ggccttcacg tctgcacgat cgagagcgcg ggccttcagt ttttcatgtg
       61 tgagcatttt ttatctccct catccgccgt cgggctgtct caagctctct gagcggggtt
      121 ttatctgttt tcttcctgaa ctggtggagc atgacgatct ttttaccacc catggtgcag
      181 tagaagaccc gtgccattcc ctcttcggct ttcagccgca actcaaacag gccttcaccc
      241 attgcgcgag tatgcggcat acccaggtca gggccaaatg cctccatcct gtctgaatat
      301 cggagatatc ggccaagaaa gcctgcgggc atggctagga tttcctgttg cagagattcg
      361 ctgtaatacg tgattgccca attcatgaag gcattatagc gtatatgcta tatacagtca
      421 acggggattt gatgtttcag tggtaagcgc gccataagaa ttaagaataa ggttgatcca
      481 gttcccttgc ccggctgtca ctaatattag tggctgttcc caattatttc gaaagaccgc
      541 ctggtgataa ttccttcatt gcctcacatg atacgggtgg ccgggtacaa cgattcgagc
      601 aatccgtggc atgcacgatc atgaccgcga acaccacgaa ttgttacgat aatagtgtct
      661 gtccctattt tagaacaaaa cgcttgcacc ggaaccgact gttggatagc ctgctgggtg
      721 ggcctgtacg tggtttgtcg tattccgcca gagatctgat tggtcgtaca acggggggga
      781 tgagaaatcg atgcgaacgg cgctgactat cgcgggctcc gattccggcg gcggggctgg
      841 gatccaggct gaccttaaaa ccttcgcagc actgggtgtc ttcgggacgt ccgcgattac
      901 ctcggttacg gcccagaaca cggtcagtgt ccaaggggta cacgatctgc cgccggagtt
      961 cgtcgggcgt cagatcgact cggtccttga ggatattgcc atcgacgcgg ccaagaccgg
     1021 gatgctctca agtgcagcga ttatcgaggt ggttgcggcg aaggtgaagg cgcatgcaat
     1081 cgagcggctg gtggtcgatc cggtgatggt ggccaagggc ggggcgccac tcctgcaacc
     1141 cgatgcagta aacgcgctca tcgagcacct gctaccactt gcgctcatgg tcacgcccaa
     1201 tgtgccggag gctgaggcgc tcacagggct gacgattact gagctcaatg agatgcgtca
     1261 ggcggcgcgg cagatccacg gcctcggccc gaggtacgtt gtcctcaagg ggggtcacct
     1321 gggcagtcgc gcggtcgatc tcctgttcga cggaagtagg ttcacagaac tagatgccga
     1381 gcggatcgat acgccgcaca cccacgggac cggatgcgtg ttctctgcgg ccatcgctgc
     1441 ggagttggcc aagggcagtc cggttccgga ggccgtcgcg accgccaagc gcttcatcac
     1501 ggctgccatc cgtcatgggt ttcggcttgg caagggattc ggtccgaccg atccgatcgc
     1561 ggccgccaga agcctcatac cataaaaaca gggtagaggg tttagggtat agggtaaggg
     1621 atgaactaca tacctcgcac cctgcaccga tgggacgtct caccgcaaga ggcaatggcg
     1681 atccagctca agctgcgatc tcagcttcgt ctacacggca gcggaccttt cgccacagtt
     1741 gcgggagtag atgtggcgta cgatgagacc ctgaagttga tgtttgctgg ggttgtcgtc
     1801 atgagcggcg acgggcgtga ggtgctggat gtcgccacag cggctgcgag cgtaaatttc
     1861 ccgtacatcc ccggactcct gtcgttccgg gagatgcccg cggtcatcaa ggcctggaac
     1921 ggacttaaga cgatgccgga ctgcctgatc tgtgatggcc acggccttgc gcatccccgc
     1981 cgctttgggc tcgcctgtca tctgggcctc ctgctcgatc ttccctcgat cggttgcgcc
     2041 aagagccgtc tggtggggac gcaccaggag ccgcggaagc gccgcgggag cgttgcgccg
     2101 ctgctggatc agggcgagca gatcggcgtg gtccttcgga cgaaagatgg ggtgacgccg
     2161 gtctttgtct ctcagggcga tcggatcagt ctggataccg ccgtgcagac ggtgcttgca
     2221 acctgccgcg gttatcgact cccagagccc cagcggcgtg cccatctctt ggtaacaaag
     2281 atgcgactta ccactagagc ttaggtagat aggctgaagg ctgaagggag cggtgggcct
     2341 cttacctata aaagacttga gtcttcaatc tcaactctta accgctgata gctgaatgct
     2401 gaccgctgac agctttttct ggtaaagtta gagcgtaaag gaggaaggcc acatggaatt
     2461 ggggctgcgt gggaaggtcg tgatggtgac cggcggaagc cgagggatcg gcagggcgat
     2521 cgccctcggt tgtgccgagg aggggtgccg gctcagtatc tgtggtcgag gcctgccggc
     2581 gctggaagag gtggccgaga cgatcaaagc ctctggagtc gaactcctca cggtgcccgc
     2641 cgacctcacc gatcctcatg gggccgagcg ctttgtgcgg gcgacgctcg accggtatgg
     2701 ccggatcgac gtcctggtga acaacgtcgg cgggtcgcgc cgcggggagc tggaggtcct
     2761 cttggagcag gattggcgag aggtgtacga tctgaacttc ttctcgaccc ttcggatggc
     2821 ccggctggtg gtgccggtca tgaagcggca gggcggcgga cggatcatca acatcgcctc
     2881 gatctggggg cgggagtcgg gaggggcgat gacctacaac gcctccaagg cggcggtgat
     2941 cagcctttcc aagtcgctcg ccaaggaact ggcccctcac aatatcctgg tcaacagcgt
     3001 cgcgcccggc tcgacgcttt ttccgggcgg ctcgtgggat aggcggcagc gcgagaaccc
     3061 ggctcagatc gcccagttta tcaaggacga actgccgctt ggccgattcg gccgccccga
     3121 agaggtcgcc gccgtcgtgg tcttcctggc atcccaacag gccagcctcg tgaccggcgc
     3181 gtgcgtcacc gtcgacggct gtcagtcgcg ctcgctcatc taaccgcccg cttcggccga
     3241 ttgccaccag tgaaaggctg gaacgccttg gtcgtcggca tagggcgtag tgttcgaacg
     3301 cagttctgat aatctcagac agagtagtct gctcgcgcct cgcaatctta cgtaaccttc
     3361 tcagtacgtc ttcatctgca aagactgtcg tccgtttcat cttcacctcc tgccgtaacg
     3421 catgcgtatg ctcgcatacc tttgcaagcg aattcaacga cgcctgaaca tttccctgga
     3481 cgaaccgttt cgtcttgcta ccgagagcgg ccgctacctg ttccgactcc cttccttgga
     3541 ataattgcca gttgtataga gcgacgtcac gaagagtgtg ccgcattttt tgtttgacac
     3601 gctgtggagg ggcatgttac aagcctaaat agtatgtgac aatgacttat aatatcgtat
     3661 aatatgatat actagtcaca ttagtgttag cacagtacag tgaggtcaaa gatgctgcgc
     3721 gtcgggtatc tcctcttggc gggtgatacg cgcctacggt cccgacatgg cgggtgcaga
     3781 gggtacgttc gcgagtgtag gccgattcgt cgggctagga gtcggcgcac ggagcccacg
     3841 tcgtgagtgc gagatccggc aggctctggc gggccaggtg cctgtggggc ctcagcctcg
     3901 gcctcgtcct gagcggcggc gtcatggtcc acgccctaac cccatccgat ctggcagggt
     3961 caacgcttaa cgcctccgtg cacgtgggcc gccccgtggc ccatgctgga gacaacttca
     4021 acgcggccac cgggacgccc acgacccttg acggcagtgc cagctttgac caggatgcgc
     4081 gcctgctgac gttccgttgg aggctgcagc acgcgccacg aggaagcgcg gcgaccctcg
     4141 ttgattccac ccagccgaat cccaccttca cgcccgatgt tccagggagg tataaattcg
     4201 agctggtggt caccaatggg tctcgagaca gctcgcccgc ttacgtgaag atgactgcct
     4261 ttgcgtcggg gacggcgccg ccgaacgcgc gcccaggtcg ggatcagagt ggattcgtcg
     4321 gcacgcccgt ggcactcgac ggcggtgaga gcgatgatcc cgaggacgct ctcctactct
     4381 ttgtgtggtc gttccgcagg gtcccgcctc acagcagcct gaccagcgcc gatatcgtcg
     4441 gccgcgacac cgccatgccg tcgtttatcc ccgatgtgcg gggagagtac cgactactct
     4501 tccaggtgag cgacggcgtc ctcacggatg aggcgatcgt actggtggtg gcggactccc
     4561 tagtgggacc tcacgctgat gccggacctg accagctcgt tgagggcctc ggccctatca
     4621 cgctggacgg ccgcgacagc aacgactcgg acgggaggcc ttccccagta tcctactcgt
     4681 ggcggctggt ggcccgtccc cccggtagtg cgctcaccac tgacgcgctc caacatgcga
     4741 ccacggtgac gccgaccttt acccccgacg tactgggagc ctacatcttc aggctgcagg
     4801 tcagcgacgg ctccagcact gacgaggaca acgtcctgat cgaggtggcg tcccaggtga
     4861 accacccgcc catcgcccag gacgatgccg cagtcaccga tgagaacgca ccggtcggca
     4921 tcaccgtcct cggcaacgat atggatcctg atagcgatcc cctcacgatt acggacgtca
     4981 cgcagggcgc caacgggggg acggtcgcca tcaacggcgt caccgccacc tataccccgc
     5041 ccgccaactg gagcggaacc gatagcttca cctactcgat tagcgatggg cgaggaggga
     5101 ccgacacggg aagtgtgacc gtgaccgtca accttgtcaa tgatcccccg acggtgactc
     5161 tgggggcgga gcagacgagt ggaccgctcc ccctcgtcgt tgccttcacg gccgcaacca
     5221 gcgattcgga tggcgatctg ctgacctacg cctgggactt cggcgatggc acgacgaagc
     5281 cgaccggcag cgccatcgaa agccacacct accagaccgc gggtagcttt accgccactg
     5341 taaccgtctc ggacggccaa gcgaccgcac aggcgagcct ggacatcacc ccatcaggcg
     5401 ttcctgatcc gagcactgtt gcgccgcccc tgtcacagac cgtcaccacc gatctcggga
     5461 gcgggaccgc attcctgtac tccggctcga atccgatcca aaccggggtc gcgcctggga
     5521 cgatcaacct cacgcgcgcg gcggtcctgc gcggccgggt cctgaacagc ctggggacgc
     5581 cgttgcccgg tgtcgcgatc ggtattctca atcacccgga gttgggccag acccagagc
//
LOCUS       DATMXI010000409         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_183726, whole genome shotgun sequence.
ACCESSION   DATMXI010000409 DATMXI010000000
VERSION     DATMXI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183726"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1283)
                     /locus_tag="VN444_07945"
     CDS             complement(21..1283)
                     /locus_tag="VN444_07945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWR21773.1"
                     /translation="MTERMVRELGEAAHLAARALALVVPEIKNRALMTIADALWNGRA
                     EILSANELDLAEARLQHHSPAVLDRLALDERRIEKMAAGVRQVAVLSDPVGEITRMWP
                     RPNGLLVGRMRVPLGVIGVIYEARPGVTADAAALCLKSGNAVILKGGREAIRSNRVIS
                     SLLADAAMGSGLPKGCVAFIDSVDREAVAHLLQLTGLVDLIIPRGGEELIRAVQRTST
                     IPILAHDKGLCHTYVDEGADLTMAEEIAFNAKVDRPGVCNAMESLLVHERVASLFLPR
                     IVRRLQEAGVEIRGCPRTKALVQGIAPATEVDWDTEYLDLILSVKVVGSFEEAVAHIA
                     AHGSGLAEAIVTADHSRAMRFLREVDAGAVFVNASTRFTDGGEFGMGAEMGISTQKLH
                     ARGPVGLTELTCEKFIVFGEGQVRDSTK"
     gene            complement(1373..>2113)
                     /gene="proB"
                     /locus_tag="VN444_07950"
     CDS             complement(1373..>2113)
                     /gene="proB"
                     /locus_tag="VN444_07950"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWR21774.1"
                     /translation="LLHLGVLPVINENDTVAVEEIKFGDNDHLSVLVATLLGADLLVI
                     LTDLDGLYTADPRRDPNARLVHEVPKRSTGLHFWADDSGTGLGTGGMVTKVEAARRAA
                     ASGIPTIIANGLVEGILGRIVRGEAVGTIFQASASRMRSRKRWLAFATERRGRITVDA
                     GAKEALIRHGKSLLPSGVVSVDGGFEGGEVVSLCDTLGVEFARGVANYDAEQVERIKG
                     IRSNQIEDTLGVKLFDEVVHRDNLVILE"
BASE COUNT          429 a          733 c          546 g          405 t
ORIGIN      
        1 cacctgagta gttacgaaca tcatttggtt gagtctcgca cctgaccctc cccgaacacg
       61 atgaacttct cacaggttag ctccgtgaga ccgaccgggc cccgggcgtg aagcttctgc
      121 gtgctgatcc ccatttcagc acccatcccg aactcgccgc cgtcagtgaa acgggtggag
      181 gcattgacga acacggcgcc ggcatccacc tcccggagga agcgcatggc ccggctgtga
      241 tccgctgtga cgatcgcctc ggccagacca gagccgtgcg cagcgatgtg cgccacagcc
      301 tcttcgaacg agccgaccac cttgaccgac aggatcaggt cgagatactc ggtatcccag
      361 tctacctcgg tggcaggtgc gataccttga accagggcct ttgttcgagg gcaaccccgg
      421 atctcaacac cagcctcctg caaccgcctg acgattcgtg gcaaaaagag agaggccacc
      481 cgctcatgaa ccagcaggct ctccatggcg ttgcagacgc ccggtcgatc gaccttggca
      541 ttaaaggcaa tctcctctgc catggtgaga tcggccccct catcaacgta ggtgtggcag
      601 agccctttgt cgtgggccag gatcggaatg gttgaggtcc gttgcactgc ccggatcaac
      661 tcctcgcctc ctctggggat aatcaggtcc acgagcccgg tgagctgaag caagtgcgcc
      721 actgcctccc ggtcaaccga atcgatgaag gctacacacc ccttgggcag gccgcttccc
      781 atcgcggcat ccgcaagcag actgctgata accctattgc tgcggatcgc ctctcgaccg
      841 cctttcagaa tgacggcatt ccccgacttc aagcagaggg ccgccgcgtc ggcggtgacc
      901 cctggcctgg cctcgtagat aacgccgatg accccaagtg gtacccgcat ccggccaacg
      961 agcagaccgt ttggccgagg ccacatcctg gtaatctccc cgacaggatc ggagagtacg
     1021 gccacctgcc ggacgccggc tgccatcttc tcaatccgcc tttcgtccag agccagccga
     1081 tccagaaccg caggcgagtg atgctgtaac ctggcctcgg cgagatcgag ctcattcgct
     1141 gagaggatct ccgctcggcc gttccacagc gcatccgcta tagtcatcag cgctcgattc
     1201 tttatctccg gcaccaccag ggccaaggcc cgagccgcaa gatgcgctgc ctcccccagt
     1261 tctctgacca tccgctccgt catactacac cccgcactaa acgtctcagt tcccagatcc
     1321 ctaacgttga acctcgcacc ttgagccttg aacgttgaac ttttgtcttc ccttattcaa
     1381 gaatcaccaa attatcccgg tgaactacct catcgaacag cttgacacca agcgtatcct
     1441 cgatttggtt gctcctgatg cccttgatcc gttccacctg ttcagcatca tagttcgcta
     1501 cacccctggc gaattctacg ccgagggtgt cacagaggct gactacctcg cctccctcga
     1561 acccgccatc gaccgacaca accccagaag ggagaaggct cttgccgtga cggatcagcg
     1621 cctccttagc cccggcatcc acagtaatcc gtcccctacg ctccgtcgca aaagctaacc
     1681 agcgctttcg gctccgcatc ctggaagcag acgcctgaaa gatggtgccg accgcctcgc
     1741 cacggacgat ccgccccagg atcccctcca caaggccgtt cgcgatgatg gtggggatgc
     1801 cagaggctgc cgcccggcgc gccgcttcca cctttgtcac catcccaccg gtaccgagcc
     1861 cggtcccaga atcgtcggcc caaaagtgga gccccgtaga ccgtttgggc acctcatgga
     1921 caagccgcgc gttgggatct ctcctcggat cagccgtgta cagcccatcc aggtctgtca
     1981 ggatgacgag cagatccgcc ccgaggagcg tcgctactag taccgaaaga tggtcattat
     2041 ccccgaactt aatctcctcc actgcgaccg tgtcgttctc gttaatgacg gggagtaccc
     2101 ccaagtggag cag
//
LOCUS       DATMXI010000410         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_207420, whole genome shotgun sequence.
ACCESSION   DATMXI010000410 DATMXI010000000
VERSION     DATMXI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207420"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VN444_07955"
     CDS             complement(<1..967)
                     /locus_tag="VN444_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HWR21775.1"
                     /translation="MNRSVQWPVLLTAGLLLFLATSAWGHAFPDRSEPRVGWTVQAPP
                     PRVRIWFDGVLEPVFSTIKVVNAAHQHVDNGNGRVNPADHTVLEASLPPLPPGQYQIL
                     WSVVAHDGHRTEGDISFTIGPGLSDRPRVTSTPATLPHLFVRWLNFIGISILIGTLAF
                     RLLLVRSVVLQRQEFETVAQPLRRIELSSIVLVALASVGELILRTQMMGGGRLAEIHV
                     TLPVVLLQTHFGVVWLVRLGFVGFLGLIWGLGWTTAPQSQRAIFLSLSAAMVIALTTS
                     LSGHAADWGDLAMPVLIDWIHLVAVSVWIGGLFTFGLIFQRFAAPP"
     gene            complement(1139..4312)
                     /locus_tag="VN444_07960"
     CDS             complement(1139..4312)
                     /locus_tag="VN444_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWR21776.1"
                     /translation="MFAWIIERSLSNRLLVVVAFVVLLIVGLFTIRRITLDVFPEFAP
                     PQVVIQTEAPSLPPQDVEFLITFPIESAINGTPGVEVIRSKSTAGLSSIVVVFNWGAN
                     IYTARQLVSERLQAVKDRLPPGTKSPMMLPITSAVGWLVKYALTSDSVSLMDLRTISD
                     WDIRNRLLAIPGVASVVSIGGEVKQYQVLLSSDKLFQYDVTVQEVLDTVKRANVSLPG
                     GFLVTPGQEYVITGAGRISSLEELKQTKVAQRGMAPIRLDQVATVRFGPEFKRGDATW
                     EGKPAVIGTVSKLFGVDTVTVTYQVEQALEEIRKTLPPGVELNTQVFRQASFIESSIR
                     NLRQAILEGGVVVSIVVIFFLFNVRASLITLTALPTSLISGVLLLKAFGIGINAMTLG
                     GLAIAIGEVVDDAIVDVENIVRRLRLNKEKTSPDPPLKVIYEASVEIRHAVVHATWIV
                     LIVFMPIFLLGGVEGRIFTPLGLAYTSAIFASLLVAVTLVPALAAMLLIRRGAAQPEQ
                     ESVTVQGMKQAYEALLRRALHHPRWVMASCVALIAGSLAVVPFFGRSFLPEFREGNFI
                     LQVMMLPGVSLPESMRVGERIVRLLKQHPEVVSVAQRAGRAELDEEALPPNVSEFDIA
                     LRYGTREPDVLLRAIRQDLEKVPGVATVLGQFISHRLDEVLSGIRAQIAIKLYGPDLA
                     VLRAKGQQVEQIMRGIRGITDLLLEPLINVPGVRIAVDREEASRLGLDVGDILETTEV
                     AFGGTAVSRIVEGQRAFDLFVWFDEASRKDRASMLNLLIDAPEGRKIPLRLVADLQIV
                     DSPFMINRERLQRRIVVQANVADQDLVSVIHEVQEKISREVQLPTGYFIEYGGQFKSE
                     QEATRVLMVYGGLAVVGIFLVLYKAFNSSRAALLVMANLPLALIGGVAAILLTGMVTS
                     VPSLIGFISVFGIASRNGIILVSHYRRLRAEGASKEQAIIQGSKDRLAPVLMTAAAAA
                     LGLLPLLFGEAAGKELERPLAQVILGGLFTSTVLNMIVVPTLFMRFGWEREEIFQEQL
                     ALERGDLFRPGPPPSDIPTVTPNRTAP"
BASE COUNT          942 a         1371 c         1319 g          777 t
ORIGIN      
        1 caggaggggc ggcgaagcgc tggaatatca atccaaacgt gaagagaccg ccgatccaga
       61 cagagaccgc aaccaggtga atccagtcga tgaggacagg catcgccagg tctccccagt
      121 ccgcggcatg ccccgacagg ctcgttgtca gggcgatgac catggctgcg gaaagggaga
      181 gaaaaatggc ccgttgactc tgcggtgcgg tcgtccaccc gagtccccag atgagcccca
      241 ggaacccaac aaaaccgagc cggacgagcc agacgacgcc aaagtgggtc tgtaggagta
      301 cgaccgggag ggtgacgtgt atctcggcaa ggcgcccgcc acccatcatc tgcgttctca
      361 ggatgagttc gccgacactc gccagtgcca caagtacgat cgaactcagc tctattcgcc
      421 gaagaggttg cgcaaccgtc tcgaattctt gacgttggag cacaactgac cgaacgagga
      481 gcaagcgaaa ggcgagcgta ccaatcagta tcgagatgcc gataaagtta agccagcgaa
      541 caaagagatg cggaagcgtg gcaggtgtgc tggtgacccg gggccgatcg gacaggccgg
      601 gaccgatggt aaacgaaata tcgccttcgg tacggtggcc gtcgtgggca acaacgctcc
      661 acaatatctg gtattgccct ggtggcagag gtgggagaga agcctccaag acggtgtggt
      721 cggcagggtt cactctgccg ttcccgttgt caacgtgctg atgggcggca ttgactacct
      781 tgatcgtact gaagacgggc tccagtactc cgtcgaacca gatcctcacg cgcggaggcg
      841 gcgcctggac cgtccaccct acccgtggct ccgagcggtc gggaaaggcg tggccccaag
      901 ctgatgtggc cagaaagagc agcaggcctg ctgtcagcag gacaggccat tgtactgatc
      961 tattcatcgc agcactccat acgctttcgg cgcgccgatg ggcattgaag tccgtgccct
     1021 acaccccctc accttcatcc tctcccccgg tgggggagag gcgtcttttt atttgcccct
     1081 cttatgtgcc cctcgcccct tgggggagag ggtgcgggtg aggggattgt ctacaatatc
     1141 atggcgcagt ccggtttggg gtcactgtcg gaatatctga aggtggggga cccggtcgaa
     1201 acaggtcgcc ccgctccaga gccaactgct cctggaagat ttcctcccgc tcccagccga
     1261 accgcatgaa cagggttggc accacaatca tattcaggac ggtcgaggta aagagccctc
     1321 ccagaatgac ctgagccagt ggccgctcca actccttccc ggcggcctcg ccgaagagga
     1381 gggggagcag gcctaacgca gccgcggcgg ccgtcatcag gaccggcgcc agccgatctt
     1441 tcgatccttg gatgatggcc tgctctttcg atgcgccttc ggcccgaagc cgccggtagt
     1501 ggctgacgag gatgatcccg ttccgggacg cgatcccaaa gacactgata aacccgatca
     1561 gcgaggggac gctcgtgacc atccccgtga gcaggatagc cgccaccccg ccgatcaggg
     1621 ctaaggggag attggccatg acgaggaggg cggcccggga cgaattgaag gctttgtaca
     1681 gtacgagaaa aatcccgacc accgcaagtc cgccatacac catcaggacc ctcgttgcct
     1741 cctgctcact tttaaactgc ccgccgtact cgataaagta gcctgtggga agctggacct
     1801 ccctggaaat cttctcttgg acctcatgga tcacgctgac aaggtcctga tccgccacat
     1861 tcgcctgcac aacgatccgc cgctgcaacc gctcccgatt gatcataaag gggctgtcca
     1921 cgatctggag atcggccacc agcctgagtg ggatctttcg cccctcgggc gcatcgatga
     1981 ggaggttgag catagaggcg cggtccttgc gggaggcctc gtcaaaccag acaaacaggt
     2041 caaacgctcg ctgcccttcc acgatgcggg agaccgccgt cccgccgaag gcgacttcgg
     2101 tcgtctccag gatgtcgcct acgtccaggc cgagacggga ggcttcctct cgatcgaccg
     2161 cgatgcgaac ccctggaaca ttgatgaggg gctcgaggag cagatctgtg attcccctga
     2221 tgccccgcat gatctgctcc acctgttgtc cctttgcccg cagcacggcc aggtcgggcc
     2281 cgtagagctt aatagcaatc tgagcccgga tgccggagag gacctcgtcc agccggtgcg
     2341 agatgaactg cccaagaacg gtagccacac cagggacctt ctccagatcc tgccggatgg
     2401 cgcgcagcag cacatccggc tcccgcgtgc catatcgaag agcaatgtcg aattcgctga
     2461 cattcggcgg cagcgcctcc tcgtccaatt ccgcccgacc cgcccgctgg gcgacggaga
     2521 ccacctcggg atgctgcttc agcaagcgga cgatccgctc ccccacgcgc atcgattcag
     2581 gaagcgagac cccaggcagc atcatcactt gcaggatgaa gtttccctcg cggaactcag
     2641 gaaggaagga gcggccgaag aatgggacaa cggccagact cccggcaatg agcgcaacac
     2701 agctcgccat cacccatcgg gggtggtgca gcgcccggcg caggagcgcc tcataggctt
     2761 gcttcatgcc ctggacagtc acactctctt gttctggttg ggctgcccca cgacggatca
     2821 ggagcatggc ggccaaagcc ggaactaacg tcaccgcgac caggagagag gcgaagatcg
     2881 cggaggtgta ggcaagtccg agcggggtga agatccgccc ctccacccca cccagcaaaa
     2941 agatcggcat aaagacgatc aggacaatcc aggtcgcatg gaccacagcg tgtcggatct
     3001 ctactgacgc ttcgtagatc accttcaagg ggggatcggg gctggtcttc tccttattga
     3061 gcctgagccg gcggacaata ttctcgacat ccacaatcgc gtcatcaacg acctcgccga
     3121 tggcgatggc caaaccccca agggtcatgg cgttgatgcc tatcccgaac gccttgagca
     3181 gtagcacccc ggagatgagg gaagtcggca gcgccgtcaa ggtgatgagg gacgcccgga
     3241 cattgaagag gaagaagatg acgacgatgc tcaccaccac ccccccctcc aggatcgcct
     3301 ggcgaaggtt cctgatggag gattcgatga agctcgcctg acggaagacc tgcgtattca
     3361 gttcgacccc tggcggtagc gtcttgcgga tctcctccag cgcctgctcc acctggtacg
     3421 tgaccgtcac ggtatcgacc ccaaagagct ttgagacggt tccgatcaca gcgggcttcc
     3481 cttcccaggt cgcgtctccc cgtttgaact ccggaccgaa gcgaacagtc gccacctgat
     3541 ccagccggat cggtgccatg cctcgctggg ccaccttggt ctgcttgagc tcctcgagtg
     3601 acgaaatccg gccggccccg gtgatgacgt actcctggcc gggtgtcacc aggaagcctc
     3661 cggggaggct aacattcgcc ctcttgacgg tatcgaggac ctcctgaacg gtcacgtcgt
     3721 attgaaacag cttgtccgaa gacagcagta cctgatactg cttcacctcc ccaccgatgg
     3781 agaccactga ggccacgcca gggatcgcca acagccggtt gcggatgtcc caatccgaga
     3841 ttgtgcgaag gtccatcagg gagacgctgt cgcttgtcag cgcatatttg accagccagc
     3901 ccaccgctga ggtgatcgga agcatcatgg gactcttggt ccccggtggg agccgatcct
     3961 tgaccgcctg gaggcgttcg ctcacaagct gccgcgccgt gtagatgttc gccccccagt
     4021 tgaagaccac aacgatgctt gagagcccgg cggtcgattt ggatcggatc acctccactc
     4081 ccggagtccc gttaatcgcg gactcgatcg ggaaggtgat gagaaactcg acgtcctgcg
     4141 gtggcaggct gggggcctcg gtttgaatga cgacctgggg aggggcaaac tcagggaaga
     4201 catcgagtgt gatccggcgt atcgtgaaca ggccgacaat cagcaggacc acaaaggcga
     4261 ccaccacgag gaggcgattg ctcagagacc gctcgatgat ccaggcaaac atctcagctc
     4321 ctgtccacta aatcccctcg tgcccccctt taccaaaggg gggggtgggg ggatttcgca
     4381 ccctcgcgta ttaatgcgta tgcccgccc
//
LOCUS       DATMXI010000411         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_126235, whole genome shotgun sequence.
ACCESSION   DATMXI010000411 DATMXI010000000
VERSION     DATMXI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126235"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1750..2316)
                     /locus_tag="VN444_07965"
     CDS             complement(1750..2316)
                     /locus_tag="VN444_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21777.1"
                     /translation="MGTEVPLQHPLRRLFDALTEKSFTEHLGWADPNVTDYLSNLLVE
                     FTHTDRLYKIRNRQNRPVDTVVDLLFESEVLLEAQSMDRERDVHQHIGDFTLFMAGLF
                     PEYLRRLKTAGLIYHKDFLVDYMKTGKRSYGIVAQIADGPSSENPPLFRKLSDNFELC
                     VTGLGFVRSDLDRMKDPAYRQARNLLLN"
     gene            complement(2597..3046)
                     /gene="dtd"
                     /locus_tag="VN444_07970"
     CDS             complement(2597..3046)
                     /gene="dtd"
                     /locus_tag="VN444_07970"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWR21778.1"
                     /translation="MKAVIQRVTRAAVHVDGQTVGQIESGLLILLGVAKGDGESDGRY
                     LVEKICTLRIFSDEDGKMNRSLVDIGGSVLLVSQFTLMGRTANGRRPSFEEAAPPEEA
                     KRLYEQVAAGFRRHGTHVETGVFAAHMHVELLNDGPVTFVLDSRGSS"
     gene            complement(3050..3574)
                     /locus_tag="VN444_07975"
     CDS             complement(3050..3574)
                     /locus_tag="VN444_07975"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWR21779.1"
                     /translation="MRQAIVFKSRWGWMGVSETTQGIDVVVLPKSSQRAVLTELQLPE
                     TALLEGRTSPRLREAQAQLTGYLAGKRRSFDLPLDLTRGTNFQQKVWQTLCRVSYGRL
                     RSYQWVAVRIGGSEYARAVGNAVGANPMPIVIPCHRIVSQDKSLGGFSGGLDTKRKLL
                     TLEGTLAELRPEPS"
     gene            complement(3583..>3945)
                     /locus_tag="VN444_07980"
     CDS             complement(3583..>3945)
                     /locus_tag="VN444_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21780.1"
                     /translation="MSSLGAAGLALATALAAMVNGGILIVVLHRRLGGVDWGSVGRSL
                     LRVLIASVPLVIVCAWVTTAQVWTHPGEWVEKSIMLCMAIGVSAVGYLGIHALLRSEE
                     LKLVWEMLRKKLGRVTAH"
BASE COUNT          886 a         1112 c         1006 g          941 t
ORIGIN      
        1 ccgcaagtcc gaacgtcacg gcgtggtcgg actctcggcc caatgtacat ccgagtccgt
       61 tgacatagaa ccgtttcgcc gccgccacat cgtgcgtggg aaaggccagg tgaaagagcg
      121 cgtctttcat gggattctgt ccttagaaca accgggcggc catcatccac tgaaccgggg
      181 ctctgtctgg caccttacga gcctgagaga ccctctagcg tagtcttatt gtgggtgatg
      241 cttcaagtct taccacgaga cgacaggaga tcggtcacac ggtttctctg ctacaactcg
      301 ctgaatgagc tgcggctgtt gcctgctaca gaagcatatg tcggatgggg ggagggttac
      361 tcttaaatct gatgttgggc ggtggctgtt cgtgcctttt ccccttgacg gtagttggag
      421 cctacttcgc tttcatggca caccacacac acaataaacc accaagtacc atgcttgcca
      481 caaccgctgt gatactaacg accatccaca acatgaacaa ttcgtataac atatgtatat
      541 acttccccct agtacctgct ctttgtcggc ctcgcaatca ccgcaaattc cgcagcgata
      601 attatcgatc gaaggaccgc ggtgccttta gattcgggca cttcaagcca cctcatgcga
      661 taagcttatt ttatcatagg ggagggcaag atgagaagaa gactgccgct tgcgactttc
      721 tgcttaaccc tcttacaata gtgtctctgc gaggctctcc atgcccttct cggctagcct
      781 cttcctctcc ttttatgcgc attgttccgc cagctacaac acaggtccat gccagggcca
      841 atgacctact ttctacctat tcacactgtt ctgtttatta tcctgctaca agtaagcttt
      901 caaccactga ttcacgcttc ctctgatccg gagagccttc ttccctaccc agtaattatt
      961 gctcctggcg caatttttag cagaacggcg tccggagtat ttttgggttc acccacgaca
     1021 atctatttga ttacggcacg gcatgttctc gtgagtgata ccaaagcact tgtaaagcct
     1081 tttgcgttgg ttcggggaaa tgcaaccgac ccccatgagg atcggggcta tatgctggag
     1141 ttggatcttg acgctcttca acggtcgggt aactggaaag aggatcttga ccacgatgtt
     1201 ctcgttgtac gattcatgtg ggactcgaag cttatggagg gggttcaggt caagtacgaa
     1261 ccaccatcag gaccagcaat ggtacccttt ccctctgtgc tgcgtctgca tgacgtagct
     1321 ttgcagaggc cgtgtacctc gcaggatatc gggtttcaat cattccaagg ggcgcggaga
     1381 cctctgagct gaatattcca aaaattcgag agggcaagac tcttctcaag gatgagcaga
     1441 agggccagat tgtttctaat taccttgccg ttggcggcga cagtggtgca ccagttttca
     1501 tgttcgcacg gggaggtgcc gccaagttaa tcggtataca cattggtgga caggattgca
     1561 ttacccccga aggcccattg aaatgtgcga ttgcgactcc gatggatttt atctttgcac
     1621 tcatctcgac atttaagtag agctgcacga cgcctccggc aagaagctga aaagtccgtc
     1681 aggatttacc ccggcttgtc gggtcccgca actcaatatt tcacgagttg tctgagaagc
     1741 ggaagctggt caattgagga gaaggtttcg agcctgccgg tacgcaggat ctttcatgcg
     1801 gtccagatct gaccgcacga atcccagtcc agtgacgcag agctcgaagt tgtcggagag
     1861 ttttcggaac agcggagggt tctctgatga cggaccgtca gctatttgcg cgacgatccc
     1921 gtaagagcgc tttcccgttt tcatgtagtc gacgaggaaa tccttatggt agatcaatcc
     1981 cgctgtcttg aggcgtcgca gatattcagg aaagagccct gccatgaaca acgtgaaatc
     2041 accgatatgt tgatgcacgt cccgttcgcg atccatcgac tgtgcttcga gcaacacttc
     2101 cgattcgaac aacaggtcta ccaccgtgtc gactgggcgg ttttgccgat tcctaatttt
     2161 atagagtcgg tcagtgtggg taaattcgac gagcaggttg gacaggtagt ccgtgacgtt
     2221 ggggtcggcc catccgagat gttccgtgaa gcttttctcg gtcagggcgt cgaagagccg
     2281 tcggagcgga tgctgtaatg ggacttccgt tcccatattc ctctcccaaa gattccgaat
     2341 acgtgcaaat tcagtatacc aggtgttgat cgaagcgacg ttgtcggtcc tatggcctct
     2401 atcggtcctc acaagacaga cttgagcata gatctgaact ggcagaacct gcaagcagta
     2461 caaaagtagt agatcaacag gccgtcacca tcatttgtga tctgtctggc agcttttact
     2521 catcaatcca gaccaaggac attgacgaga cgggcccgaa acggcctcct gaatcggtgg
     2581 gcttctgagc agtctgtcag cttgagcctc gactatcgag cacaaaggtc accggcccgt
     2641 cgttcagcaa ctcgacgtgc atgtgtgcgg caaaaacccc ggtttctaca tgcgttccat
     2701 ggcggcgaaa acctgccgcg acctgctcgt agagtcgctt ggcttcttct ggtggtgccg
     2761 cctcctcaaa gctcgggcga cggccattcg ccgtcctgcc catcaacgta aactgagaaa
     2821 ccagcaaaac agatcccccg atgtccacca gcgagcggtt catctttccg tcttcatcgg
     2881 aaaaaattcg aagagtgcaa atcttttcga cgagataccg gccgtccgat tctccgtcgc
     2941 cttttgccac acccagcagg ataaggagac cggattcgat ctgccccacg gtctggccat
     3001 caacgtgaac cgccgccctg gtcacccgct gaatcacggc cttcatcggt tatgatggtt
     3061 ccggtcggag ttcagccaag gttccctcga gcgtgagcag ctttcgtttc gtatctaagc
     3121 cgcctgagaa tccgccgagc gacttatcct ggcttacaat ccgatggcaa ggaatcacaa
     3181 tcggcatcgg attcgctcca acggcattac cgacggcacg agcgtactca ctacctccga
     3241 tacggacagc cacccactga taggatcgaa gccggccata ggagacacgg caaagggtct
     3301 gccagacctt ctgctggaag ttcgttcccc ttgtgaggtc cagtggcagg tcgaaggacc
     3361 tccgtttccc tgccagatag ccggttaatt gtgcctgtgc ctcccgtagt cgtggagaag
     3421 tgcggccttc caacaaggcg gtctcgggca attgcaactc tgttagcacg gctcgctgcg
     3481 aggacttcgg caataccaca acatcgatcc cctgtgtcgt ttctgaaacg cccatccagc
     3541 cccatcgtga cttgaagacg atcgcttgtc gcatgtgtga cctcaatgag ccgtgactcg
     3601 gcccagtttt ttccgcaaca tttcccatac caacttgagc tcttctgacc tcagtagggc
     3661 gtgaatgccc agatatccga cggcactcac tccaatcgcc atacagagca tgatcgactt
     3721 ctcgacccat tcaccgggat gggtccagac ctgtgccgtg gtcacccagg cacagactat
     3781 gaccaacggc acggatgcga tcagcacacg gagtaatgag cgtcccactg atccccagtc
     3841 cacaccgcct agtcgacggt gcagcacgac gatcaagatg cctccgttta ccattgccgc
     3901 caatgctgtg gccagggcga ggcctgccgc gccaagagac gacat
//
LOCUS       DATMXI010000412         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_271592, whole genome shotgun sequence.
ACCESSION   DATMXI010000412 DATMXI010000000
VERSION     DATMXI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271592"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(510..710)
                     /locus_tag="VN444_07985"
     CDS             complement(510..710)
                     /locus_tag="VN444_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21781.1"
                     /translation="MSKVEQLKTEIERLPKKEFTELVRWLSEKDWERWDKEIEADSKA
                     GKLDFLIREALDAKDKGTLKNL"
     gene            complement(1113..1571)
                     /locus_tag="VN444_07990"
     CDS             complement(1113..1571)
                     /locus_tag="VN444_07990"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HWR21782.1"
                     /translation="MKTNSGSELRTTRRPVLPDDACPECGSPMSEKKGRLKLPVNGEE
                     IAVAGSPHMSCPKCHEVVLRLDDARKLRQRALEIYRQTYGLLSADEIRSIRERFGLTQ
                     AELARLLRLGANTISRWEAGRNVQTASMDMLLRVIRDLPGSLDYLRKYAA"
     gene            complement(1558..1881)
                     /locus_tag="VN444_07995"
     CDS             complement(1558..1881)
                     /locus_tag="VN444_07995"
                     /inference="COORDINATES: protein motif:HMM:NF027059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HWR21783.1"
                     /translation="MRRRWLTAVLARIRESTARRKVRFTMKALQGLAALGVGLDEEDA
                     CDVLAHLDPSDFVERLASQKTGEWMYVFKPSVGGVAVYVKVVLRADCIVISFHEEEGH
                     IDEDE"
BASE COUNT          522 a          747 c          630 g          574 t
ORIGIN      
        1 atcttgactg ccgagatggg acgaacactg gtcgtgctca ctacctcatg cttcagaagc
       61 tccgggaggg tcctctggaa acaggacagc cctttggggc ttcccgtacc ccaaaaggct
      121 gtgacagatt cctcccgctg agcgctggtt cggttggcgt atcggttctc atctcgctct
      181 gtgccatgcc cggcacatcg gatagcttga gcgacgcgcg atttctgcgc atgagactcg
      241 aggctgtagt tagccctgtt gcttgatcag tcgctcatat tcatcatggg gaccgatcca
      301 gacccagatg aaatccaccc catcctcgac tgacagcgcc cgatgatgag gcccagctct
      361 cacagaccaa agcttgccga cctttttgaa gtgcagcgaa gggtgcgcgg ggttttcttg
      421 cagaagatcg aagtttcgtc gagccactct ctgaacggtt tgtggaaggc gcgcgaagca
      481 accccaaaaa cgagctgttg tgcgatgttt cacaggtttt tgagagttcc cttgtctttc
      541 gcgtcgagtg cctcacgtat tagaaaatcc agtttcccgg ccttggagtc agcctcgatt
      601 tctttgtccc acctctccca atccttctct gacagccacc tgacaagttc ggtaaactcc
      661 ttcttgggaa gcctctcgat ttcggtcttg agttgttcta cttttgacat gatcttacct
      721 cccgccagaa cttcggaata ccaattcacc gaagctgcaa tcctggaaca caccagttct
      781 cagctagact cagtatgcca cgcctacgcc ttgagcgcta ccctcgctgc gcggtagtgg
      841 gctaacctat cgagtatgct ggtcagggga tccccattag ctaatgttgc agtcatggag
      901 gattctattg tgttcttcag tagttagcaa gagatatctt gactgccgag atgggacaca
      961 cactggtcgt gctcacaatc tcgtgcttca gaagctccag aagggtcccc tggaaacagc
     1021 acagcccctt ggggctcacc ggaccccaaa gggctgcgac agattcctcc tgctgagcgc
     1081 tggtccggtt ggcgtatagg ccgtgacaag cgctacgcgg cgtactttcg taggtagtcg
     1141 agactcccgg gcaggtcgcg aatcactcga agcagcatgt ccatcgatgc tgtctgtacg
     1201 tttcgacccg cctcccagcg cgagatggta ttcgccccaa gacgcagcag ccgcgcgagt
     1261 tcagcctgtg taagaccgaa gcgctcacgg atcgaacgaa tctcatcggc agacaagagc
     1321 ccataggttt gccgataaat ctccagagct cgctgtcgaa gctttctcgc gtcatcgagc
     1381 cgcaagacaa cttcgtggca cttcggacag ctcatgtgtg gcgacccggc caccgcaatc
     1441 tcctcaccgt tcaccggcag ttttagcctg cccttcttct cgctcatcgg gctgccgcac
     1501 tccgggcaag catcgtcagg caacaccgga cgtcgcgtcg ttcgtagctc agacccacta
     1561 ttcgtcttca tcgatgtgac cctcttcttc gtgaaacgag atgacaatac aatcagcgcg
     1621 aaggacaacc ttcacgtaga cggcgactcc accgacggac ggcttgaaca catacatcca
     1681 ttcgcccgtc ttctgcgagg ccaggcgctc cacgaaatcg ctcgggtcaa ggtgtgccaa
     1741 cacatcgcag gcgtcttcct catcgagtcc cacgccgagg gccgccaacc cctggagcgc
     1801 cttcatcgta aagcgtacct tccgccgagc cgtcgactcg cggattctgg caaggaccgc
     1861 tgtgagccat cgtcgtcgca ttcacgcttt ggtgaaatta tgcatttggt gaaggttgag
     1921 tgtcaactcc aatcactacc ccgcggccgc cggcaaccgt tattcgacat ttctcatgtc
     1981 attgccagag cttcagcctt ggcttcgata gaccgcacaa gatcctggat ggtatcgact
     2041 tccttttctt cgaaatacgc ttctccgcat tgccgcacac ccacactgga accgcatcaa
     2101 gcatcaggtg gcaacctttg cggtcaatat gaaaaggcgc tgcgggccgc agcagctgcc
     2161 gcccgaaccc gatttcggat gtcctcgcgt ctatccgcgc ttatatgcta ctgaaaaacc
     2221 cttcgaggtt caatcatcta tgaagtcgag ctatagagta tgctggtcag cggagctcca
     2281 ttgcctcttt tttactgtca gggcggattc tattgtgtgt tcagtagtca gcaagcgata
     2341 tctgaactgc cgagatgaga cgagcacagc acgtattgtc aatggatttt ctcgcagagg
     2401 agaacgccgc acccccttcc cttcgcacaa gtgctcccgc acaccaggca aggccagggg
     2461 agcagggaga gtc
//
LOCUS       DATMXI010000413         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_183946, whole genome shotgun sequence.
ACCESSION   DATMXI010000413 DATMXI010000000
VERSION     DATMXI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183946"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /gene="murA"
                     /locus_tag="VN444_08000"
     CDS             <1..1255
                     /gene="murA"
                     /locus_tag="VN444_08000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWR21784.1"
                     /translation="DRLIIKGGVPLRGQVEAAGAKNAALPEMVASLLTGESIRLHRVP
                     QLGDVKTTIGLLSHLGVSVEGCGQETLSLRADRIGQFEAPYQLVKTMRASVLVLGPLL
                     ARFGRAPVSLPGGCAIGTRPINLHLAALEKMGATVSLDAGYVEAKAPRLKGARITFDG
                     QTVTGTENLMMAATLADGVTVLENAACEPEVTDLAALLNRMGASIEGAGTPTISIEGA
                     PHLHGAEHEVIPDRIEIGTFMVAAAITGGDVTINRCLPSHLDAVTEKLRETGVVVEET
                     DCSIRVCGDGQVRGVNIRTHPYPGFATDMQAQFMALMSSAQGSSVITETVFENRFMHV
                     NELLRMGADIKVIGTTAFVHGIPMLSGAPVMATDLRASASLVLAGLAAQGITTVSRVY
                     HLDRGYESIEQKLLGLGASIERVTE"
     gene            1317..1994
                     /gene="hisG"
                     /locus_tag="VN444_08005"
     CDS             1317..1994
                     /gene="hisG"
                     /locus_tag="VN444_08005"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWR21785.1"
                     /translation="MEALNNNVIAIALPKGRLLTPILELFEGIGFSCVRGFSDSRRLI
                     CEDRDRQLRFLILRPADIPTYVERGAADMGIAGKDQLLEQRRDVYEPLDLRFGACRLV
                     VAEPAELHKQDDPHSWSYLRVATKYPNLAEAYFSRKGIQAEIIKLTGSLELAPLVGLA
                     ERIVDLVETGQTLKENGLVEVEEIAKSTARLIVNRASLKTKYRRVQDLIEQMRAQIET
                     HAARVCV"
     gene            1991..>2221
                     /locus_tag="VN444_08010"
     CDS             1991..>2221
                     /locus_tag="VN444_08010"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWR21786.1"
                     /translation="MKVLHAGSRECTLFFSQLRSRQGTIPAEVEQAVRDILEGIRTGG
                     DASLFAQTERFDGVRLDATSVRVRPEELEEAYA"
BASE COUNT          441 a          621 c          725 g          434 t
ORIGIN      
        1 ggatagactg attattaagg gtggggtgcc cctcagagga caggtagagg ctgccggcgc
       61 caagaacgca gccctgcctg agatggtagc ctctttgctc acaggggagt cgatccgact
      121 tcatcgcgtt ccgcaactgg gcgacgtgaa gacgaccatc ggcctgctca gccatttagg
      181 tgtgagcgtc gaaggatgtg gtcaggagac cctatccctt cgcgctgatc gcatcggtca
      241 gtttgaagcg ccctaccaac tggtcaagac gatgcgggca tcggtactgg tgcttggccc
      301 gctgctggcc cgcttcgggc gggcaccagt ctctttgcct ggagggtgcg cgatcgggac
      361 gagaccgatc aatctgcacc tcgcggctct tgagaagatg ggtgcaacgg tcagcctgga
      421 tgccgggtac gtggaggcga aggcgccacg tttgaagggc gccaggatca cgttcgacgg
      481 ccagaccgtc accggcaccg agaatctgat gatggccgcc accctggcgg atggggtcac
      541 cgtgttggaa aatgccgcgt gcgagccaga ggtcactgac ctggccgccc tgctgaatcg
      601 catgggggcg agcatcgagg gcgccgggac tccgacgatc agcatcgagg gtgccccaca
      661 tctgcacggg gcagagcatg aggtgattcc agacaggatc gagatcggga ccttcatggt
      721 tgcggccgcg atcaccggtg gcgatgtcac catcaatcgc tgcctgccgt cccacctgga
      781 tgcggtcaca gagaaactgc gtgagacagg cgtagttgtg gaggagacgg actgcagcat
      841 tcgagtctgt ggagatgggc aagtgcgcgg agtgaatatc aggacccacc cctaccccgg
      901 ttttgctacc gatatgcagg cgcagttcat ggccctcatg tccagcgccc aggggagcag
      961 cgtcatcacc gaaacggtct ttgaaaaccg attcatgcac gtgaatgagc tgctgaggat
     1021 gggtgcggat atcaaggtga ttggcactac cgcgtttgtc cacgggattc ctatgttgtc
     1081 gggcgcgccg gtcatggcca ccgacctgcg ggcgagcgct tcgctggttc tggcgggact
     1141 ggccgcccag gggatcacga ccgtctcgcg tgtctaccat ctggaccggg gatacgagtc
     1201 gattgagcag aagctcctcg gcctcggcgc cagcatcgag cgagttacag agtaatagaa
     1261 gagggcttcg ggtttttggt tcttacgcga aacccgaaac cctcgtaaca cgagatatgg
     1321 aagctctaaa caacaacgtg atcgccattg cgctgccgaa gggtcggttg ctgaccccta
     1381 tccttgagct ctttgagggg atcggctttt cttgcgtgag gggcttctct gactcacggc
     1441 gcttgatctg cgaggatcgc gatcggcaac tccgattcct catactccgg cctgccgaca
     1501 tcccgaccta tgtggagcgc ggggccgccg acatgggtat cgcggggaaa gaccagctcc
     1561 tcgagcagcg ccgggatgtc tatgagccgc tggatctcag gttcggcgcc tgtcggcttg
     1621 tcgtggccga gccggccgag ctccataagc aggatgatcc ccattcctgg tcgtatctga
     1681 gggtggccac caaatatccg aaccttgcag aagcgtactt cagtcgcaaa gggatccagg
     1741 ccgagatcat caaactgacc ggctcccttg aacttgcgcc ccttgtagga ctggccgagc
     1801 ggattgttga cctcgtagag accgggcaga ccttgaagga gaacgggttg gtggaggttg
     1861 aggagatcgc caaatctacg gcgcggctca ttgtcaatcg cgcgagcctc aagacgaagt
     1921 atcgcagggt tcaggacctg atcgaacaga tgcgagcgca gattgagacg catgcggcaa
     1981 gggtgtgtgt atgaaggtac tccatgctgg atcgcgcgag tgtacgctgt tcttctcgca
     2041 gctcagatcc cgacagggta ccatacccgc cgaggttgag caggccgttc gcgacatcct
     2101 ggaagggata cggacaggcg gcgatgcctc cctctttgcg cagacggagc ggttcgatgg
     2161 tgtccgcctc gatgccacct cagtgcgggt aaggccggag gagctcgaag aggcatacgc
     2221 t
//
LOCUS       DATMXI010000414         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_71262, whole genome shotgun sequence.
ACCESSION   DATMXI010000414 DATMXI010000000
VERSION     DATMXI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71262"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1488
                     /locus_tag="VN444_08015"
     CDS             466..1488
                     /locus_tag="VN444_08015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR21787.1"
                     /translation="MDGVSEMQESQSPLISVVIPSYNRAALLPRAITSVLTQTFSNLE
                     CIVVDDGSTDHTEQVLAGFPDPRLRVVRLPGNGGYSRANNTGIQVARGELIAFLDSDD
                     EWRPEKLECQVARLREYGDPMATVVYCRCMVRDALSGRMWRRPHRIYEGDVFPYLLEG
                     WHPTTPSLFLVDRRSLLKVGGFSETLPSAQDYDLWLRLAEASFRFVATDEVLVVKHEY
                     SDDQMSDDPTTPVRTGEILDHRWGPVIRRHLGWVGYRRWRKRRWDVVQWAHFRRIKIA
                     IAHGQRLEAWRTWVGMCRLLSHSIQYVCSAFLMIVLGFRAYCLLVHAVEAVRRSVRRS
                     QDMAGA"
     gene            1523..3364
                     /locus_tag="VN444_08020"
     CDS             1523..3364
                     /locus_tag="VN444_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR21788.1"
                     /translation="MSSDGASSRRSLKTSVIALLANMQWAFRLTWSISPLLVSGVVIS
                     TCGRALVPAGLALTARGLINGLVDLTHRGSDDFSALIPWLLMGAGLAIVQAISDVGSR
                     YLIQRLTDEIRLTITADILKHAAGLDIACFEDHRFHDVMERARQDTAQHFSQFLINVI
                     GVINNGLQLVSLLLILVVIEPFITAVLAPVALPYLLFQLRLAGVRHVKEQTRTTGRRW
                     THYFVRLLTDPQSAAEVKLLDLAPLLINRYRTLITEFRDQDRQLHFRSFVGGSAFALS
                     AAVVFYLILSWVVYRALQSGLTVGDVAIYAGASSALRSNLSNVIQSMSNTVEHALFVS
                     NLKEFLAIQPRIHDTGSTIPVSRQGEIELTHVSFSYPGSSQLILSDISLHIEPGETVA
                     LVGENGAGKTTLAKLIARLYEPDQGYIALDGVDIRQLPVAYLHSQLAFVLQGFGRYEA
                     TAADNIAYGDWRRLLHDQDQVKAIAKEANIQEMIERMPQTYGTMLGKMFGGHDLSTGQ
                     WQQLAVARAFARDACVLILDEPTASLDARAEHAIFSRFRELLKGRTTILISHRFSTVS
                     MADRILVLDQGRLIEQGTHQELMAHNGHYATLYNLQERQMPVNGAAS"
     gene            complement(3404..4129)
                     /locus_tag="VN444_08025"
     CDS             complement(3404..4129)
                     /locus_tag="VN444_08025"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HWR21789.1"
                     /translation="MIIEVAFGWLDPACRSASGRAVAVIDVIRATTTITMALHQGCAG
                     VIPVRTLSEARTVARRLGQGALLAGERGAERAAGFDLGNSPAEYGRERVKGKTVVLTT
                     TNGTRTFQAVSGAQAIIACSFLNVSAAAHWLAATGLDILIACAGRYGRFCLEDVVGSG
                     MLIDRVLSVGDRPIDLTDAARAAHQLFSHYRGDLLGMLRGCEWGGEIIRKGFGADLEI
                     CAQVDLTDIVPVMRKDRLVGKRT"
BASE COUNT          878 a         1243 c         1192 g          929 t
ORIGIN      
        1 cgacagatgc cttctacgag tcaatgaggg actggcgcac atcttacaag gtatttatga
       61 cgttcggggg cgtgaatgtc cacccggatg gacagcatct gatggcctca aagtcgacgg
      121 accgaaaagc actagtcgcc tcactcaggt tgccggagat gcctaactgg gtcacctcac
      181 tgacatatac gtcccacgaa cgccgcatat ttccaaaatt ccacgccaga ttcgacatgg
      241 gccataaata cactcagcgc ctgcacaccc cggtgtcgga tggtctactt gcctcctgtc
      301 gccgttataa ggtcgaagat gtgtgttatc tccatgtcaa gttttgtaaa cgcgaaccgt
      361 actccaatta ttcttcggaa ttagctcaac tgatctcttc aaacgttgcc gtagggcagc
      421 ctctgcgtcc tgaatggtca gacctgctct gtcggtggag gaagaatgga tggcgtcagt
      481 gagatgcaag agtcccagtc gccccttatc agcgtcgtca ttcccagcta caaccgggcc
      541 gcactcctgc cgcgggctat cacgagcgtc ctcacccaga ccttctccaa cctggagtgt
      601 atcgtggtgg acgacggttc cacggatcac accgagcagg ttctggccgg attcccggat
      661 ccccgtctcc gagtggtacg acttccaggc aatggtgggt atagccgggc caataatacc
      721 gggattcagg tggcccgcgg tgagctcatc gccttcttgg acagcgacga tgagtggcgg
      781 ccggagaagc tggagtgtca ggtcgcccgc ttgcgcgagt acggggaccc tatggcgacg
      841 gtggtgtact gtcggtgtat ggtccgcgat gcgctgtccg ggcggatgtg gcgcagaccc
      901 cataggatct acgaggggga cgtgttcccg tacctgctgg agggatggca cccgactacg
      961 ccttccctat ttctggtcga ccgacgctcg ttgctcaaag tgggaggctt cagcgaaact
     1021 ctcccgtctg cgcaggatta tgacctctgg ctccggcttg ccgaggcatc ttttcggttc
     1081 gtggcgacgg atgaggtgtt ggtcgtcaaa catgagtaca gcgatgacca gatgtcggat
     1141 gatccgacga ctccggtaag aaccggcgag attctggatc acaggtgggg gccggtgatc
     1201 cggcgtcatc tcgggtgggt aggctatcgc cgctggagaa agcgccgctg ggacgttgtt
     1261 cagtgggcgc atttcaggcg cattaagatc gcgatcgctc atggccaacg cctcgaggct
     1321 tggcgaacct gggtggggat gtgtcggctc ctctcccact cgatacagta tgtctgcagc
     1381 gcattcctga tgatcgtact gggattccgc gcgtactgcc tcctggtaca tgctgtggag
     1441 gcagtgcgcc ggagcgttcg tcgctctcag gacatggccg gggcttgata cgttcatggg
     1501 aacaccaagc atgacacacc gcatgagttc tgacggcgca tcctcccgcc gatccttgaa
     1561 gacctccgtc attgccctgc tcgccaacat gcaatgggcg ttccgactga cctggtctat
     1621 cagccctctg ttggtatccg gcgtggtcat ttcaacctgc ggtcgagccc tcgtaccggc
     1681 cggtctcgcg ctgactgcca gaggattgat taatgggctg gtagatctta cacaccgagg
     1741 atccgatgac ttctcagcgt taatcccctg gcttctcatg ggtgcaggac tggccatcgt
     1801 gcaggccata agcgatgtcg gcagccgcta cttgatacaa cgcctgaccg acgagattcg
     1861 gctcacaatc actgcggata tcttgaaaca tgcagccggc ctggatatcg cctgttttga
     1921 agaccatcga tttcatgatg tgatggaacg cgccagacag gatactgctc aacacttctc
     1981 tcaatttctg atcaacgtca ttggagtaat caacaatggt ctccagttgg tctcgctgtt
     2041 actgattctt gtcgtcatcg aacccttcat cacagcggtt ctcgcgccgg tggcgcttcc
     2101 ttacttactc ttccagttac ggctcgccgg ggtgcgtcac gtcaaggagc agaccaggac
     2161 caccggacgc cgatggacac actatttcgt ccgtttactg acagatcccc agtcggctgc
     2221 cgaggtaaaa ctgttggacc tcgctccttt actgatcaat cgctaccgta cgctcatcac
     2281 tgagttcagg gatcaagacc gtcagttgca ttttcgcagc ttcgtggggg gcagcgcatt
     2341 cgccttgtcg gcggcggttg tgttctatct gatcctgagc tgggtagtat atcgagcact
     2401 acaatcgggg ctgacggtcg gggacgtggc catctatgcc ggagcgtcct ccgcccttcg
     2461 cagtaatctg tcaaatgtta tccagtcgat gagcaacacg gtagaacacg cgctgttcgt
     2521 ctccaacctc aaagagtttc tggctatcca accaaggatc catgataccg ggagcacgat
     2581 cccggtatct cgacagggcg agatcgagtt gacacacgtc tcattcagct atccaggatc
     2641 aagccagttg atcttgtcgg atatctccct ccatatcgaa ccgggcgaaa ccgtcgcctt
     2701 ggtcggggag aacggggcgg gaaaaacgac gctggcaaaa ctgattgccc gattatatga
     2761 accggatcaa ggatacatcg ccttagacgg ggtggatatt cgacagttac cggttgcgta
     2821 ccttcatagc cagcttgcct ttgtcctgca aggattcggt cgatatgagg ccacagccgc
     2881 cgataatatc gcgtatgggg actggcggcg cctcctgcac gaccaggacc aggtcaaggc
     2941 gattgcaaaa gaagcgaaca tacaagagat gatcgaaagg atgcctcaga cctacgggac
     3001 catgctgggc aagatgttcg gcgggcacga cctgtcgaca ggtcagtggc aacaactggc
     3061 ggtggcgcgc gcgttcgcga gagatgcctg cgtactcatc ctggatgaac ctacggcgag
     3121 cctggatgcg cgggctgagc acgcgatctt ttctcgattt cgggaactgc ttaagggccg
     3181 cacaacaatc ctcatctctc atcggttttc caccgtctct atggccgatc gtatactggt
     3241 gctcgaccag ggacgtctca ttgagcaggg gacccatcag gagttgatgg cgcacaatgg
     3301 gcattatgcg acactctata atcttcagga acgacagatg cccgtcaatg gtgccgcttc
     3361 atgaatgaat gaccggctgc tatcggtcat tatcgacggt ctctcatgtg cgctttccta
     3421 cgagccggtc tttgcgcatc acgggcacga tgtcggtcag atcgacttgc gcgcagatct
     3481 ccaggtccgc cccaaagcct ttacgaataa tctctcctcc ccattcgcag cctctgagca
     3541 tcccaagcag gtcgcctcga tagtgtgaaa aaagctgatg ggcggccctg gcggcatcgg
     3601 tgagatcaat cggacgatcg ccaacgctga gaacccgatc gatcaacatc ccgctgccca
     3661 caacgtcttc cagacagaaa cggccgtatc ggcccgcaca cgcaatgagg atgtcgagcc
     3721 cggtggctgc aagccaatgc gcggcggcgg atacattcag gaacgagcag gcgatgattg
     3781 cctgagcccc agagacagct tggaatgtac gggtcccgtt cgtggtcgtc aggacaaccg
     3841 tcttcccctt gacgcgctca cgcccgtact cagccggaga attcccgagg tcaaagcctg
     3901 ccgcccgttc tgcgccccgt tctcctgcaa gcagggcccc ttggccaagc ctccgtgcca
     3961 cggtcctggc ctcgctgagc gtccgcacag gaatgacacc ggcgcaaccc tggtgcagcg
     4021 ccatcgtgat ggtcgtcgtc gctcgtatca cgtcgatgac ggctaccgct cgaccggagg
     4081 cagaccggca ggctggatcc aaccaaccga atgccacctc gatgatcaca atggtgatcc
     4141 cggctcagca accatgcccg catcctgcac tgatagtatt gacacccctc tgtagacctg
     4201 ctatgaacag acggccatct cccttcagcg gaaagcttcc ac
//
LOCUS       DATMXI010000415         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_53410, whole genome shotgun sequence.
ACCESSION   DATMXI010000415 DATMXI010000000
VERSION     DATMXI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53410"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1225
                     /locus_tag="VN444_08030"
     CDS             476..1225
                     /locus_tag="VN444_08030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWR21790.1"
                     /translation="MEEGPLHSFELHDWIPISIGGLDISINKAVVMMWVVISLVAVLM
                     VIAGSARKLVPGKLQSIAEMMVDFIRSMILDTMGKDGMRFFPLIATLFLFILFCNLVG
                     LVPGSYTVTSQIVVTAVFASVVYGISLMMGFLLHGFKFLGILVPPGTPGWLLPLMIPI
                     ELMSQLARPISLAVRLFANMTAGHVILGVLFGMAISGGLLIGWLPFAFTVAIYGLEVG
                     IAFIQAYIFTILTCVYLGDAYHLHGHEEHAH"
     gene            1354..1587
                     /gene="atpE"
                     /locus_tag="VN444_08035"
     CDS             1354..1587
                     /gene="atpE"
                     /locus_tag="VN444_08035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWR21791.1"
                     /translation="MDLAAAGLIGMGCAAAGFAGAGVGIGYIFGKVIEVVARQPEAEA
                     RVTKYMWIGFALVEAIALYGLVVVFIIMGLRKG"
     gene            1602..2108
                     /gene="atpF"
                     /locus_tag="VN444_08040"
     CDS             1602..2108
                     /gene="atpF"
                     /locus_tag="VN444_08040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWR21792.1"
                     /translation="MPQFESHFFSSLIFWEIVSFVILLFVLYKFAFPGILSVLEEREK
                     KIKDSLDQAERHRSEAELKLKEYEAKLNSAAKEAEGILAAAKDRAQRLMEENEQRMTV
                     EAERIKGDATREIDQERRKAIQDIRAQTTNLALMVAEKVVQRSLNEADHRKFADEALE
                     ALSKSYQR"
     gene            complement(2127..2936)
                     /gene="larE"
                     /locus_tag="VN444_08045"
     CDS             complement(2127..2936)
                     /gene="larE"
                     /locus_tag="VN444_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWR21793.1"
                     /translation="MLTESLQVKLNTLRRMILDLQSVLVAFSGGIDSTVVLKIAHEQL
                     GDHALAVTAVSPTFPQVELDVAKRVASEIGVRHELVHTDQLEVAAFVQNDATRCFHCK
                     TDLYQLLDGLREPHASRWIVDGTNLDDLGDDRPGIKAAREWGVRSPLVEASLSKSDVR
                     ALAQALGLSNWDKPAAACLSSRIPRGTPITIDSLRRVEQAEDILQAEGFRHVRVREHG
                     DVARIEVGAEEFSRLNQPTLRAHISARLRQAGFRFVCVDLEGYRPGGISLG"
     gene            complement(2968..4335)
                     /locus_tag="VN444_08050"
     CDS             complement(2968..4335)
                     /locus_tag="VN444_08050"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWR21794.1"
                     /translation="MPTMSQPPISQGRQAILVLVVGLCAFLLGSRPVIAAEIAPTKFV
                     TANGMTVIVLEQHFLPIVEIHALIKAGSAQDPADKAGLANLVASLLDEGTTTRTSKQL
                     SEQIDFVGGALEAKASEDFTTASARVLKKDIELGFTLLADMLQHPAFAKQEFERIRTQ
                     ILGEMASDNDDPGHIAMKAFNQLVFHGHPYRWPVNGTEETLHKITLADVQAFYGREYS
                     PSQVILTIVGDITLEQATALTQTHFGAWKKGAVAARNMKNPAPVERRTVQLIEKDLTQ
                     STIILGHAGIPRTHPDFYTVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSHFDARL
                     MPGSFWVNLQTKTETTNQAIAGVLSEIKSIRDAPVSDQELAEAKSFLIGSFPLRFDST
                     AKLANVLAQVEFYGLGFEYFLEYSKWIDRVTKEDVQRVAKQYLDPKRYALVVVGNTAK
                     AKVKR"
BASE COUNT          940 a         1101 c         1329 g         1042 t
ORIGIN      
        1 gcggaggacc gctcaagaat gatgcttggg agaaacgtgc catgggtgaa cgtgtagcgc
       61 tcttcacaga cgcccgatgc actataccgc acggttcctg ttgtatcaca gcgcaagcca
      121 cagcttgaca acattaggac gattctggtt gaattgcgag cccgccaccg gcagagtcat
      181 tgcatcgtgg gctgcattgc actctcctcc gtgggtacga catgccccct tcacaggatc
      241 cattgtacac ggggcttggc caagcggtca ggatcgcaag cgatctggtc gctgcgctga
      301 ttgtcggagg aggattggga tgggtgtgtg acacctacct tttggattct actccctggg
      361 ggatggttgg cggtcttata ttgggtgtcg tgacagggat cagaaacgca taccggtctg
      421 tgcagcgctg gccgaaggcc taacgtcttt cgtatagaga gagcaagagc acgcgatgga
      481 agaaggccct cttcattctt tcgaacttca cgactggatc ccgatctcga taggaggatt
      541 ggatatctcc atcaataagg ccgttgtgat gatgtgggtt gtgatttccc tggtggccgt
      601 gcttatggtc atcgcaggat cggcgcgtaa gctggtgcct ggtaagctgc agagcatagc
      661 ggaaatgatg gtggatttca ttcggagcat gatcttggac acgatgggta aggatggaat
      721 gcgatttttc cccctcatcg cgaccttgtt tctctttatt cttttttgca atcttgtcgg
      781 gttggtccct ggcagctata cggtgacgag tcagattgtc gtgacagccg tgttcgcctc
      841 cgtggtgtat gggatcagtc tgatgatggg gttcttgtta cacggattta agttcctggg
      901 aattcttgta ccgccgggca cgcctggctg gctgctccca ttgatgatac cgattgagct
      961 gatgagtcag ttggcgaggc ccatttcact ggccgttcgg ttatttgcaa acatgacggc
     1021 gggacacgtc attctcggag ttctgttcgg catggcaatc agcggcggac tattgatcgg
     1081 gtggttaccc tttgcattca cggttgcgat ctatgggctg gaagtcggga tcgcgtttat
     1141 ccaagcctat attttcacca tattgacctg cgtttactta ggggacgcct accatctaca
     1201 tggccacgag gagcacgcgc actgaccgtc gtaacctctt cgtcagactc gtgcggaatg
     1261 cctcaaggtg gtcgatggtt acggatctgc cgggagacaa attgagagcg tgttcaagta
     1321 tattgagttt tcataaagga ggatagacaa acaatggatt tagcagcagc agggttgata
     1381 gggatgggtt gtgccgcagc agggtttgcc ggagcaggtg tggggatcgg ctacatcttc
     1441 gggaaggtca ttgaagtggt ggcgcgccag cctgaggcgg aagctcgtgt cacaaagtat
     1501 atgtggattg ggttcgcgct ggtggaagcc atcgcactgt atggcctggt cgttgtgttc
     1561 atcattatgg gactccgcaa ggggtagggt aggaagctca catgccgcag tttgaatctc
     1621 atttcttttc ttccctgatt ttttgggaaa tcgtatcgtt cgtcattctc cttttcgtct
     1681 tatacaagtt tgcttttccg ggtatcttga gcgtcttgga agagcgggaa aagaaaatca
     1741 aagacagtct cgatcaggcc gagcggcatc gctcagaagc ggagttgaag ctcaaggagt
     1801 acgaagccaa attgaactcc gccgcgaaag aggcggaagg aatcctggcc gcggcgaagg
     1861 atcgggcaca gcggctcatg gaggaaaatg agcagcggat gacggttgaa gctgagcgga
     1921 tcaagggcga tgcgacgcgt gaaatcgatc aagagcggcg caaggcgatt caggatattc
     1981 gggcccaaac caccaatttg gcattgatgg ttgcggagaa ggtcgtgcag cgaagtctga
     2041 acgaggcgga ccaccggaaa tttgcagacg aggcgctgga ggccttgtca aagtcctacc
     2101 aacgataatg tctgaacctg gcggggtcac ccaaggctga tcccgccagg cctgtaccct
     2161 tccaaatcca cacagacgaa gcgaaatcct gcctgacgga gacgagcgct gatatgcgcg
     2221 cgtagagtgg gctgattcaa ccgagaaaac tcctccgccc caacctcgat tcgcgctacg
     2281 tctccatgct cccgtacacg tacgtggcga aacccctcag cctgcagaat gtcttcggcc
     2341 tgctccacgc gacggagact gtcgattgtg atcggtgtgc cccgaggaat tctggaagaa
     2401 aggcaggcag ccgccggctt atcccaattg gagaggccta aggcctgggc gagtgctcgc
     2461 acatcggact tggagagaga ggcctcaacc agcgggcttc gcactcccca ctcgcgtgcc
     2521 gctttgattc ccggtcgatc atccccgaga tcgtcgagat tcgttccgtc cacaatccat
     2581 cgacttgcgt gcggctcgcg cagtccgtcc agcaattgat agagatcggt cttgcaatgg
     2641 aagcagcggg ttgcatcgtt ctgaacaaag gctgcgactt cgagttgatc ggtgtggacc
     2701 agttcgtgcc gaacgccaat ctccgaagct acgcgtttcg caacatcgag ctcgacctgg
     2761 ggaaacgtcg gtgagacagc agtgacagcc aacgcatggt caccaagttg ttcatgggcg
     2821 attttgagga cgacggtact gtcgattccc ccggaaaagg cgactaggac ggactgcagg
     2881 tccaaaatca tccggcggag cgtattgagc ttaacttgga gagattcggt caacatgaat
     2941 gcctgttagg gggttgtgac acagtgccta gcgcttgacc tttgccttgg cggtattgcc
     3001 caccactacc aaggcatacc gcttggggtc caggtattgt ttggcaacgc gctgcacgtc
     3061 ctccttcgtg acccggtcga tccactttga gtattccaaa aaatattcga acccaagccc
     3121 gtagaactcc acttgggcaa gcacgttcgc gagtttcgcc gtggagtcaa atcgtaacgg
     3181 gaagctgcca atcaagaatg atttggcctc ggccaattcc tggtcggata cgggagcgtc
     3241 acgaatggat ttgatttcgg acaggactcc ggcaatggct tgattcgtcg tctccgtctt
     3301 ggtttggagg ttcacccaga atgaaccggg catcagccgg gcgtcgaagt ggctcatgat
     3361 cccgtaggcc aatccctgct tgtcacgaat tgagtccatt aaccgggaag aaaacccacc
     3421 agcccccagg atgtaattca tcaccgtaac cgtataaaaa tcagggtgcg tgcgtgggat
     3481 gcctgcatga ccaagtatga tggttgattg ggtgagatct ttctcaatga gttggaccgt
     3541 tcttcgttcg accggagcgg gatttttcat gttccgtgcc gccacggctc cttttttcca
     3601 ggctccaaaa tgggtctgtg tcagcgcagt cgcttgttca agggtaatat cgccgacaat
     3661 ggtcaagatg acctgggaag gggaatattc ccgtccgtag aaggcctgca cgtcggccag
     3721 ggtgatcttg tggagcgtct cctctgttcc attgacaggc caacggtagg ggtgcccgtg
     3781 gaagacaagc tggttgaacg ccttcatggc aatatggccg gggtcgtcat tatcgcttgc
     3841 catctcgccc aggatttggg tgcgaatccg ctcgaattcc tgcttagcga acgcggggtg
     3901 ttgcagcatg tctgcgagca gggtaaagcc aagctcgata tctttcttga gtacgcgggc
     3961 agaggcggtg gtaaagtcct ctgaagcctt ggcttccagg gctccgccga cgaaatcgat
     4021 ttgttccgac agctgttttg acgtgcgcgt ggtcgttcct tcatccagga gactggcaac
     4081 gaggtttgcg agtcctgctt tgtcagcagg atcttgggcg gagccggctt tgatcagtgc
     4141 gtggatttca acgatgggga ggaaatgctg ctcgaggacg atcaccgtca tgccgttggc
     4201 agtcacaaat ttggtcggcg cgatttcggc cgcgatgacg ggacggcttc ccaagaggaa
     4261 ggcacagagc cccaccacga gaaccaggat cgcttgtctt ccttgcgaga taggaggttg
     4321 actcatcgtt ggcatgacta gggcccaccc tggtgcacgg cggcggaggg cgcttctggc
     4381 tgtttggaag gcagggggat caaggtcccg ac
//
LOCUS       DATMXI010000416         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_115467, whole genome shotgun sequence.
ACCESSION   DATMXI010000416 DATMXI010000000
VERSION     DATMXI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115467"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VN444_08055"
     CDS             complement(<1..939)
                     /locus_tag="VN444_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR21795.1"
                     /translation="MNKRYVWIASVVALFLVVAVILVPIFSSSKKASTQVIFSDLMDL
                     VESGQIAGTVIFDENNHTIYAHVTEGQHLRTIYSKEATGPLQELLKKKGIRFAIEAPS
                     GGSIWLSLLFNFLPFLVIIGLFVMMSRRAQQMGGGGGPMSFGKSKAKLHDATKPNVTF
                     ADVAGEEEPKEELLEVIEFLKHPQKFQALHAKIPRGLLLVGPPGCGKTLLAKAVAGEA
                     SVPFFSLSGSEFVEVFVGVGASRVRDLFEQAKKNVPCLVFIDEIDAVGRHRGAGLGGG
                     NDEREQTLNQLLVAMDGFENNMGIIVIAATNRPDILD"
     gene            complement(1542..1796)
                     /locus_tag="VN444_08060"
     CDS             complement(1542..1796)
                     /locus_tag="VN444_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21796.1"
                     /translation="MKFLTLAKVRPGAWARLENDLPAACYEGMKSAYVTYGQYDLVFE
                     WEAKDMDTANRIMKHMAGSDLLTSETLVVASTLKEFGKVQ"
     gene            complement(1813..>2021)
                     /locus_tag="VN444_08065"
     CDS             complement(1813..>2021)
                     /locus_tag="VN444_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21797.1"
                     /translation="PALYLTIASGVVLMTMEISTIGLQWLFMGKGATVLLKPSGSYCP
                     DRGGSGVTSPRENAGHATSSSLVE"
BASE COUNT          450 a          571 c          560 g          440 t
ORIGIN      
        1 gtcgaggatg tcgggccggt tggtggcggc gatcacgatg atccccatat tgttctcgaa
       61 gccgtccatc gcaaccagga gctggttcaa tgtctgctca cgttcatcat ttccaccgcc
      121 caggcctgcc ccccggtggc ggcccacggc atcaatttcg tcgatgaaga ccaggcaggg
      181 gacgttcttc ttggcttgct cgaacagatc gcggacgcgg ctggcgccaa cccctacgaa
      241 gacctccacg aactcggatc cagagagtga gaagaagggt acgctggcct ccccggcgac
      301 cgccttagca aggagcgtct tcccgcagcc tggggggccc accagcaaca gaccccttgg
      361 gatcttagcg tgtagagcct gaaacttttg ggggtgcttt aagaactcaa tgacctccaa
      421 cagctcctcc ttcggctctt cctccccggc cacgtccgcg aaggtcacat tgggcttggt
      481 cgcgtcgtgc agtttcgcct tggatttgcc gaacgacatc gggccgccgc cccctcccat
      541 ctgctgcgcc cggcggctca tcatcacaaa caggccgata atcacgagga acggtaagaa
      601 gttgaacaga aggctgagcc agatagagcc ccccgatggc gcctcgatcg caaagcgaat
      661 ccccttcttt ttcaatagct cttgcagggg cccagtcgcc tccttgctgt agatcgtccg
      721 aaggtgctgc ccctccgtga catgggcgta gatcgtgtga ttgttttcat cgaagatcac
      781 cgtcccggcg atctgtcctg attccactag atccatgaga tcactgaaga tgacctgggt
      841 ggaggctttc ttggaactcg agaagatcgg gacaaggatt acggcgacta caaggaataa
      901 ggcgaccacg ctcgcaatcc atacgtaacg cttgttcacg gcaatgacct ccttcgcgtc
      961 ataccgacat accgaagccg agcgtcaagc atccgggacg ttatggcgga caaatgaaac
     1021 atcttatggc cagggtctat catccgactc tcactacact gctcaaccca tcgttgtctt
     1081 cttatcgaca tagccaagtt tagagtaagt cagtgccctg agcaactggc tgcatcgaga
     1141 cttatacaaa acatcttcag gttaaagcaa gcatatgagt ttgtcaagcc caacctatag
     1201 gttttcgtag agtcgagggt gcgggaagtt gggagggggc gcagagaatg gcgcgacgcg
     1261 atctgattcg gaggggtatg ccgaaggagt acgtaatctc aaaggggctg tgtcgtttgc
     1321 gctcgcaacg gaacagtggg cctgccaagt accggtacgg ctgatcagtc ggatggtcag
     1381 cgcttccttc gaactccagc gctgaccgag ggaggagact ctcgtgccga acgtcgggga
     1441 ttatgacgga aggagcggag atggcgcgac agcaggtgac tatagatata gaagagagct
     1501 tgacgacccc taggcgctgt ggctctaagg gttggtcaga cttactgtac cttaccgaat
     1561 tccttcagag tggaggcgac tactaaggtt tcagatgtga gtaggtctga tccagccata
     1621 tgcttcatga tcctatttgc cgtgtccata tccttggctt cccattcgaa aacaagatcg
     1681 tattggccgt aggtgacata agcggacttc atcccctcgt aacaggcggc aggaagatcg
     1741 ttttccagcc tggcccatgc ccccggtctt accttggcta aagtaaggaa cttcatgatt
     1801 ggctaccctc cattattcga ctaaggacga tgaggttgcg tgtccggcgt tctctctcgg
     1861 cgaggtcacg ccagagcctc cccgatctgg acaatacgac cctgaaggct tgagaagcac
     1921 cgtggcccct ttgcccatga agagccactg caatccgatg gtggagatct ccatcgtcat
     1981 cagaacgacc ccgctcgcga tcgtcaggta cagcgcaggt a
//
LOCUS       DATMXI010000417         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_206358, whole genome shotgun sequence.
ACCESSION   DATMXI010000417 DATMXI010000000
VERSION     DATMXI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206358"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VN444_08070"
     CDS             complement(<1..176)
                     /locus_tag="VN444_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWR21798.1"
                     /translation="MSDEKMSFFSHLEELRKRILISLAAIGIGFIATFNYSETILRVL
                     KRPLTTDLIFSRTYP"
     gene            complement(169..456)
                     /locus_tag="VN444_08075"
     CDS             complement(169..456)
                     /locus_tag="VN444_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWR21799.1"
                     /translation="MFGLGMQELIVIFVIALLVFGPKKLPDLARSLGRGVAEFKRASD
                     ELKEGLSAELSAEEEKASTADYQPPQAAEAEAPPAVGQEDKPKGTHETRNV"
     gene            complement(507..1124)
                     /locus_tag="VN444_08080"
     CDS             complement(507..1124)
                     /locus_tag="VN444_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWR21800.1"
                     /translation="MFRLIPREEKFFELFEEAATNILEGAKVLAQMTDERGAELQEYW
                     KRLEELEHVGDKITHQIIRKLNRTFITPIDREGIHSLAVALDDVMDLIEASAARMSLY
                     KIKQPTEEARKLAQVILKSAEEMVKAVSNLERMDDVIKHCIEINRLENMADEISREAI
                     ADLFDEEHDPMDVIKWKEIYETMETTTDRCEDVANIVESVALKRA"
     gene            complement(1156..1566)
                     /locus_tag="VN444_08085"
     CDS             complement(1156..1566)
                     /locus_tag="VN444_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWR21801.1"
                     /translation="MQTNGGKDRRMLSEINITPLVDVTLVLLIIFMVTTPMLQRGTDI
                     QLPTAQASQVKEEERITLTVTRDSRILVNNEEVPRKELETRLKSMTGSGKERTLYLRG
                     DARVPYGFVIDVMDAIKSSGIETVGMITERAGSR"
     gene            complement(1578..2255)
                     /locus_tag="VN444_08090"
     CDS             complement(1578..2255)
                     /locus_tag="VN444_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWR21802.1"
                     /translation="MMGQGTFQFIAHGGITMVILAIFSIYSLAVIGERFYTYYKAQKA
                     TEQVAGKGLQYVADGKRAEALRLCEQNAGSPVAKVLQAGLLAVIDQESPVLSGKRFSR
                     RLESCKGAMQRATSAEITRLERYLGSLATLGNVSPFVGLFGTVLGIIRAFEAIAKTGS
                     GGIGTVSSGIAEALVATAAGLFVAIPAVIAYNYFVGRVKDFTTAMDNATSEMVDRLLD
                     QATHHGD"
     gene            complement(2429..>2720)
                     /locus_tag="VN444_08095"
     CDS             complement(2429..>2720)
                     /locus_tag="VN444_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWR21803.1"
                     /translation="AAIATGYTETLIARAADVVLKEGKRLALVVRESPFTAIHLEQML
                     TLARSGVRIVPPVPPFYQKARTVEDLVDQIVGRVLDQIGLHTDLPNRWRGTG"
BASE COUNT          553 a          833 c          712 g          622 t
ORIGIN      
        1 ggataggtcc tggagaaaat caggtcggtc gtcagggggc gtttcagaac acgaaggatc
       61 gtctccgaat agttaaaggt ggcaatgaat ccgatcccga tagctgccag ggagatgagg
      121 atccgctttc ggagctcctc caggtgggag aaaaacgaca tcttctcatc agacattccg
      181 cgtctcgtgc gttcccttcg gcttgtcctc ctggcctacg gccggaggcg cttcagcctc
      241 ggcggcttgc ggcggttggt aatctgccgt acttgccttt tcctcctctg ccgatagctc
      301 ggcagacagc ccttccttca gctcatcaga ggctcgtttg aactccgcca cgcctcggcc
      361 cagcgatcga gcaagatcag gcagtttctt ggggccaaag actaagagcg cgatgacaaa
      421 gatcacgatc agctcttgca tccccaatcc gaacatccta cgaacccccc actgcggcct
      481 ctttttcgat tggtagatct cgcggatcag gcgcgcttca atgctactga ttcgacgatg
      541 ttggccacat cttcacatcg gtcggtggtc gtctccatcg tctcgtaaat ctccttccac
      601 ttgatcacgt ccatcgggtc atgctcctcg tcaaagaggt cagcaatcgc ctctcggctg
      661 atctcatcag ccatgttctc cagccgattg atctcgatgc aatgctttat cacatcgtcc
      721 atccgctcca ggttcgacac cgccttgacc atctcctccg ccgatttcag gatcacctgg
      781 gcgagcttcc tggcctcctc ggtgggctgc ttgatcttgt aaaggctcat gcgggcagcc
      841 gacgcctcga tcaggtccat gacgtcatcc agcgccacgg ccaggctgtg aataccttcg
      901 cggtcgatgg gagtaatgaa ggtccggttc agctttcgga tgatctggtg ggtgatcttg
      961 tctcccacgt gctcgagttc ctcgagtcgc ttccagtatt cttgaagctc tgcgcctcgc
     1021 tcatccgtca tctgagccag gactttggcg ccctctaata tgttagtggc tgcctcctca
     1081 aagagctcaa agaacttctc ttctcgtggg atcagtctga acatgccggc tgtgggctcg
     1141 taacggtgaa gggggctatc tggaccctgc tcgctcagtg atcattccaa cagtctcgat
     1201 gccggaagat ttgatagcat ccatcacatc gatgacaaaa ccgtaaggga ccctggcatc
     1261 gccgcgtaaa tagagagtcc gctccttccc ggaaccggtc atggatttca ggcgggtctc
     1321 aagctctttc ctgggcacct cctcattatt gacgaggatg cggctgtctc tcgtcaccgt
     1381 caaggtgatg cgctcttcct ctttcacctg cgacgcctgt gctgtcggaa gctggatatc
     1441 agtcccacgc tggagcatcg gggtcgtcac catgaagatg atcaatagga caagggtcac
     1501 gtccaccaaa ggagtgatgt tgatctctga aagcattcgc cgatctttcc cgccgttagt
     1561 ctgcatgcct atcctcttta gtctccgtga tgggtggcct gatccaataa cctgtccacc
     1621 atctccgagg tcgcattgtc cattgctgtg gtaaaatcct tgaccctgcc gacaaaatag
     1681 ttgtacgcga tcaccgcggg aatagccacg aacaagcccg cggctgtcgc cacgagcgct
     1741 tcggctatcc cggatgatac cgtgccaatc ccaccggatc ccgtcttggc aatcgcctcg
     1801 aaggcccgaa tgatccccag aacggtcccg aaaaggccga caaatgggct gacattgccg
     1861 agagtcgcga gagagcccag gtatcgttcc agacgggtga tctccgcgga ggtggcccgt
     1921 tgcatagccc ccttgcacga ttcgagtcgc cgcgaaaatc gcttaccgga aaggaccgga
     1981 gattcctggt cgatgacggc caggagaccg gcctgcagga ccttggcaac cgggctgccg
     2041 gcgttctgtt cgcataagcg gagcgcttct gcccttttcc catccgccac atactgcaac
     2101 cccttcccgg cgacctgttc ggtagctttc tgagccttat agtatgtata gaaccgctcg
     2161 ccgatcaccg cgagtgagta gatagaaaat atggcgagga tcaccatcgt gatcccaccg
     2221 tgagcaataa actggaaggt cccctgcccc atcatgtcag attatcctcc aaaagggcga
     2281 ccatatacag ctcttttccc aatcgcctcg cttccctgct acaccctaca accggcttac
     2341 gcttaactga gttgtatagc atggaacggc gccgaacgga agtggataac gaatcaggtt
     2401 ggaaaaaccg ccagcggcat cccggtcgct atcccgtgcc ccgccaccga ttcggcaggt
     2461 cggtgtggag accgatctga tccagcaccc gtccgacgat ctggtccacc aaatcctcga
     2521 ccgttcgtgc cttctgatag aacggcggca ctggcggaac gatcctgacg ccagatcttg
     2581 ccaatgtcaa catctgttcc aggtggatgg cggtaaaggg gctctccctt accacaaggg
     2641 caagacgctt cccctccttc aggacaacgt cggccgctcg ggcgattaag gtctcggtgt
     2701 agccggtggc gatggccgcc
//
LOCUS       DATMXI010000418         4877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_321601, whole genome shotgun sequence.
ACCESSION   DATMXI010000418 DATMXI010000000
VERSION     DATMXI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321601"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VN444_08100"
     CDS             complement(<1..279)
                     /locus_tag="VN444_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWR21804.1"
                     /translation="MIPRYALPRMTSVWEPQNRYATWLRVELLASEAWAELGVVPREA
                     LAVIQERAGFDLNRIQEIEREVRHDVIAFVSAVAERVGPEARYLHFGLT"
     gene            complement(387..1640)
                     /locus_tag="VN444_08105"
     CDS             complement(387..1640)
                     /locus_tag="VN444_08105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWR21805.1"
                     /translation="MASFSCNRQVLPNGMVVLSERMPAVKSVSIGVWVKVGSRDEASD
                     VAGISHFIEHMLFKGTAQRSAQEIARTIDAVGGTHDASTSREGTCFFVKVLGEHLPLG
                     IDILADTFLHSRLDPKDIEREQQVVLQEIKMVEDTPDDLVHDLFAEAIWDDHPVARPI
                     LGRKETVGRFTQDDIRRHMSRFYRPDRIVVAAAGDLEHEQLVDLVAQAFDGFEGQTVH
                     ADPPPPSSSGAVRVEERDTAQLHLCLGMDALPYAHQDRYALYLLNAMLGGSMSSRLFQ
                     EIREKRGLAYSIYSYQAAYRDCGLLVIYAGISPKSSGQVVDLIRAECARLRNQPVGPS
                     DLQQAKDQLKGNLLLGLEGTSSRMTRLAKSEIYFDRTYGLDEIIAGIDAVSTDQFESL
                     TRRILRDESFAITSIGPVPQAALLS"
     gene            complement(1644..3755)
                     /gene="pnp"
                     /locus_tag="VN444_08110"
     CDS             complement(1644..3755)
                     /gene="pnp"
                     /locus_tag="VN444_08110"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWR21806.1"
                     /translation="MSCRVEQIIEGKSLSIEAGRVARQADGAVLVRYGDTVVLVTAVA
                     SKQMRQGIDFFPLTVDYQERAYAAGKIPGGFFKREGRPHEKETLTSRLIDRPLRPLFP
                     DGYRNDVQIIATVLSADQENDPDVLAVLGASAALSISPIPFLGPIGAVRVGRLDGKLV
                     LNPTYTQMEGSDIDVVVAGTRSAIVMLEAGANEVTEEVMVEVIEFGHKGIQSMIDMTL
                     ELAKELRVEKLPFKVPPVDAELGERVNRLARQGLRTLTGIADKEEQRSRRQALLDEVI
                     GQFSADPDDRKVAVKRLFEQIEHEELRRMILEDGKRADGRSLEDVRPITAEVGVLPRT
                     HGSALFTRGQTQALVTTTLGTSQDEQRLDTLEGEGTKRFMLHYNFPPFSVGEVKFMRG
                     PGRREIGHGALAERALLSALPTKEEFAYTLRIVSDILESNGSSSMATVCGASLSLMDA
                     GVPIRSAVAGVAMGLVMEKEQTAILTDIIGLEDHLGDMDFKVAGTRKGITALQLDIKT
                     QGISPSLMPKALDQARRARLHILDQMDRAIAKSRPNMSAYAPRILTIMIPVDKIRDVI
                     GPGGKVIRGIVAESGAKIDVSDDGRIEIASVDEEAAQKALAIIGRIVEVPEVGKVYRG
                     KVVRIMDFGAFVQILPGTDGLLHISQISAQRVMKVEDVLSNGEEVMVKVIDIDKSGKI
                     RLSRKEALESEEQLKAGQRSS"
     gene            complement(3752..4021)
                     /gene="rpsO"
                     /locus_tag="VN444_08115"
     CDS             complement(3752..4021)
                     /gene="rpsO"
                     /locus_tag="VN444_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWR21807.1"
                     /translation="MVKASANKQEVIGQYRLHDSDSGSPDVQIALLTGRIGYLTEHLN
                     SHKKDHHSRRGLLRLVARRRKLLDYLRSKDANRYKQIIERLGIRK"
     gene            complement(4086..>4877)
                     /locus_tag="VN444_08120"
     CDS             complement(4086..>4877)
                     /locus_tag="VN444_08120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008531 - riboflavin kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FMN
                     adenylyltransferase"
                     /protein_id="HWR21808.1"
                     /translation="PLEVVSPSKAPPLITPLSIKKDIIAALGVDLMIAATFTPRLAAT
                     LPRDFVKTDLVDRLRARFICVGYDFAFGNARAGSPEMLRRLGEEHKFWLDVVSAMTVD
                     GQVVSSTLIRGLLAAGNLRQAARYLGRPYAIRGHVERGAERGKGLGYPTANLPATADL
                     LIPDGVYGGQAWLKQGLYKALVNVGRAPTFGGETRRVEVHLLEAEEELYGETLTLFFL
                     ERLRDERRFENPSLLRQQIDSDKRQADAVFAAFPPFSPEEWTLLP"
BASE COUNT         1029 a         1536 c         1332 g          980 t
ORIGIN      
        1 cgtcagcccg aaatggagat agcgtgcctc cgggccgacc cgctccgcca ccgccgagac
       61 aaaggcgatg acatcgtggc gaacttcccg ttcgatctct tggatacggt taaggtcgaa
      121 gccggcccgc tcctggatca cggccaacgc ctcgcgcggc actacgccca gctcggccca
      181 ggcctcgctg gctaacagtt cgacccgcag ccaggtggcg tagcggttct gtggttccca
      241 cacggaggtc attcggggga gcgcatagcg aggaatcatc acgagtcctt ttggcaggag
      301 agggtacaga aaatcggatt tagggggtag gggttaaaca aatccttggc ttttgtaccc
      361 tgaaccctcc accctacacc ccgttttcag gagagtaagg ccgcttgggg aacagggccg
      421 atagaggtga tggcaaacga ctcgtcgcgc aatatccttc tcgtcagcga ctcaaactga
      481 tcggtggata ctgcatcgat cccggcgatg atctcatcca ggccataggt gcgatcgaaa
      541 tagatctctg acttggcaag gcgagtcatt cgactactcg tcccctcaag accgaggagc
      601 agattaccct tgagctgatc cttcgcctgt tggaggtcgc tgggaccgac cggctgattc
      661 cgcaggcggg cgcactccgc ccggatcagg tcgacgacct ggcctgagga cttcggactg
      721 atcccggcat aaatgaccag caggccacaa tcgcgatagg cggcctggta ggagtagatc
      781 gaatacgcta aaccccgttt ttcgcgaatc tcctgaaaga gccgagaact catgctgcca
      841 cccagcatag cgttcaacag gtaaagggca tagcggtcct ggtgggcata cggtaaggca
      901 tccataccga gacacaggtg gagctgggcc gtatcgcgct cctccaccct caccgccccg
      961 ctggaagacg gtggtggagg atcggcgtga acggtttggc cttcaaagcc atcgaacgcc
     1021 tgcgctacca aatccactag ttgctcgtgc tccagatcgc cggccgcggc taccacgatc
     1081 cgatcaggac ggtagaaacg actcatgtgg cggcggatgt cgtcttgcgt aaagcggccc
     1141 accgtctcct tccgccccag gatcggtctg gctaccggat gatcatccca gatcgcctcg
     1201 gcaaagaggt cgtgaaccag gtcatcgggg gtgtcctcca ccatcttgat ctcctggagc
     1261 accacctgct gttcccgttc aatatccttg gggtcaaggc gagaatggag gaaggtgtcg
     1321 gccaaaatat cgatacctag gggcaggtgc tcacccagga ccttgacaaa aaaacaggta
     1381 ccctcacggc tcgtggaggc gtcgtgcgtg ccaccaacag catcaatcgt cctggcgatc
     1441 tcctgcgcgc tgcgctgcgc cgtccccttg aagagcatgt gctcgatgaa gtgggagatg
     1501 ccggccacat cggaagcctc atcccgagat ccaaccttca cccacacacc aatcgagacc
     1561 gacttgacgg caggcatccg ctcgctgagg acgaccatcc cgtttggcaa tacctggcgg
     1621 ttgcaggaga aactggccat tactcaggag gacctctgtc cagccttgag ttgctcctcc
     1681 gattccaacg cctcttttcg actcaaccgg atcttcccgc tcttgtcgat gtcgatgact
     1741 ttcaccataa cctcttcccc attcgacaag acgtcttcga ccttcatcac ccgctgagca
     1801 gaaatttgag agatatgtag cagaccatcg gtgccaggaa ggatctggac gaaggcgcca
     1861 aagtccatga tcctgaccac cttccctcga tagaccttac caacctcagg gacctcgacg
     1921 atcctgccga tgatcgccaa cgccttctgc gctgcctctt cgtcaaccga agcgatctcg
     1981 atccgcccat cgtctgagac atcgatcttc gccccggatt ctgccacaat cccgcggatc
     2041 accttgccac cagggccaat gacatcacgg atcttgtcca ccggaatcat aatcgtgagg
     2101 atgcgcggag catacgcgga catgttcggc cgtgacttcg caatggcgcg gtccatctga
     2161 tccaggatgt ggagacgggc gcgcctggcc tgatccagag ccttcggcat gaggctaggg
     2221 ctgatgcctt gggtcttgat atccaattgc agcgcagtga tccctttccg ggtgccggcc
     2281 accttaaagt ccatgtcgcc aaggtgatcc tcaagcccga tgatatcggt gagaatcgcc
     2341 gtctgctcct tttccatgac gagccccatt gctaccccgg ccaccgctga ccggatcgga
     2401 accccggcat ccatcaagct gaggctcgcg ccgcagaccg tagccatgga agacgaccca
     2461 ttcgactcga ggatatcgga gacaatccga agcgtatacg caaactcctc cttcgtcggc
     2521 agcgccgaga gaagagcccg ctcggcaagg gccccgtgcc cgatttcccg ccgaccaggg
     2581 ccgcgcataa acttgacctc gccgacgctg aacggtggaa aattgtaatg gagcataaac
     2641 cgtttggtgc cctctccttc gagagtatcc aggcgctgct catcttgcga ggtgccgagt
     2701 gtagtggtca cgagggcttg agtctgacct ctggtgaaga gggccgatcc gtgagtcctt
     2761 ggcaggactc cgacctcggc ggtaatcggc cggacgtcct cgagcgacct tccgtccgct
     2821 cgcttcccat cttccaggat cattcggcgc aactcctcat gctcgatctg ctcgaacagc
     2881 ctcttgacgg caaccttccg atcgtctgga tcagcactaa actggcctat aacctcatca
     2941 aggagcgcct gccggcggct gcgctgctcc tccttgtcgg cgattccggt caaggttcga
     3001 agcccctggc gggccaggcg gttcacgcgc tcacccagct cagcatccac agggggaact
     3061 ttgaaaggca acttttcaac tcgaagctct ttcgccagct ccagcgtcat gtcaatcatg
     3121 gactggatcc ccttatggcc aaattcgatc acctccacca tcacctcttc agtgacctca
     3181 ttcgctccgg cctcaagcat cactatagcg cttcgggtcc ccgcaaccac cacatcgatg
     3241 tcagatcctt ccatctgggt ataggttgga ttgaggacca gcttgccatc caggcggccc
     3301 actcggacag ccccaatcgg cccgaggaat gggatgggcg atatgctgag agcggcagag
     3361 gcgcccaaca ccgccaggac gtcagggtcg ttttcctgat cagccgatag gaccgtggcg
     3421 ataatttgga catcatttcg atagccatcc gggaagagcg gacggagcgg gcgatcgatg
     3481 aggcgcgagg tcagtgtctc cttctcatgg ggacggccct ctcgcttaaa gaagccgccg
     3541 gggattttac ctgccgcata tgcccgttcc tggtaatcga cagtaagcgg aaagaaatcg
     3601 atcccctgcc gcatctgctt cgacgccacc gctgtaacaa gaacgaccgt gtcgccgtac
     3661 cggaccaaca ccgcgccatc ggcctgcctg gccacgcgcc cggcctcgat actcaacgat
     3721 ttcccttcga tgatttgctc gacacgacaa ctcatttacg aattcccaac ctttcgataa
     3781 tctgtttgta ccggttcgca tccttgctcc tgagataatc caggagcttc ctacgccggg
     3841 caacgaggcg gagcagccct cgccgagagt gatggtcctt cttgtggcta ttcaggtgct
     3901 cggtcaaata ccctattcgc ccggtaagga gcgcgatctg cacatccgga gagccggagt
     3961 cgctatcatg aagccgatac tgcccaatga cctcttgctt atttgcggat gccttgacca
     4021 ccaagccgtg tcctcctttc ttcttaagta gatacctaca gtcgcgagaa cgatatcata
     4081 cgccctcagg ggagtaaagt ccattcttct ggagaaaacg gcgggaaggc agcaaacacc
     4141 gcatccgcct gccgcttgtc gctgtcgatc tgttgccgga gcagtgacgg gttctcaaag
     4201 cgtcgctcgt cacgcaggcg ttcgaggaag aagagcgtga gggtctcccc gtacaactcc
     4261 tcctcagcct caagcaggtg gacttccact cgacgcgtct caccaccaaa ggtcggggcc
     4321 ctgcccacat tgacaagcgc cttatataat ccctgcttca accacgcctg gccaccgtac
     4381 acaccatccg gaatgagcag atcggctgta gcgggtaagt tggctgtcgg gtaccccagc
     4441 ccttttcccc gttcggcgcc acgctcgacg tgtccgcgaa tcgcgtaggg ccgccctaga
     4501 tagcgcgcag cctgccgcag attccctgcc gccagtaagc cgcgaatgag cgtggaactg
     4561 acgacctggc cgtctactgt catcgcagac actacatcga gccagaactt atgctcttcc
     4621 ccaagcctcc tgagcatctc tggagatccg gctctggcat tgccgaaggc gaagtcgtac
     4681 ccgacacaaa taaaccgtgc ccgaagccga tcaaccaagt cggtcttcac gaaatcgcgt
     4741 ggcagggtgg cagcaaggcg gggggtaaag gtggcggcga tcatcagatc gacgccaaga
     4801 gcggcaatga tgtccttctt gatcgagagc ggagtgatga ggggcggggc ctttgaaggg
     4861 ctcaccacct ctagcgg
//
LOCUS       DATMXI010000419         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_184433, whole genome shotgun sequence.
ACCESSION   DATMXI010000419 DATMXI010000000
VERSION     DATMXI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184433"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VN444_08125"
     CDS             <1..875
                     /locus_tag="VN444_08125"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HWR21809.1"
                     /translation="RIPVEVDYGSEFRYRNPILNERTLVVTISQSGETLDTLVSLREA
                     RRHGCKSLAICNVVGSTLSRESDGVLYTHAGPEIGVASTKAFTSQLAALYLLALALGR
                     ARGCLDGTRSQELLSDLIRLPQQMEQVLSLGPALEELAGHFYTASDFLYLGRGINYPI
                     ALEGALKLKEISYIHAEGYPAGEMKHGPIAMITEEMPVVFLAPKDRTFEKVLSNIEEV
                     KTRNGIVIALSDETDSRLMAKADHLVNIPRTSPHLMPLLLVVPLQLLAYHIAVRKGCD
                     VDQPRNLAKSVTVE"
     gene            complement(973..1380)
                     /locus_tag="VN444_08130"
     CDS             complement(973..1380)
                     /locus_tag="VN444_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWR21810.1"
                     /translation="MAVTRAMLDTSAYSAFMRGHVEVKRALQRVDEIYLNAVILGELL
                     VGFTRGKHRKENRLELQQFLASPRVSLVPLDEDTAERYALILTNLWKAGTPIPTNDIW
                     IAASAMQYGCRLLTADAHYHKVGQIIVEYFEVF"
     gene            complement(1371..1634)
                     /locus_tag="VN444_08135"
     CDS             complement(1371..1634)
                     /locus_tag="VN444_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21811.1"
                     /translation="MMKAVTLRNLPSELARIIRKKADEQHASLNKVVIGLLEQSVGVR
                     GKSGETALYHDLDALAGSWTQQETAAFNKALAKQRTIDPDLWR"
     gene            complement(1789..1941)
                     /locus_tag="VN444_08140"
     CDS             complement(1789..1941)
                     /locus_tag="VN444_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21812.1"
                     /translation="MPYEVFLRLFDRHALGEVARLVHVAPLPHGDVVSEELQWNDEKQ
                     RHQVGK"
     gene            2065..>2261
                     /locus_tag="VN444_08145"
     CDS             2065..>2261
                     /locus_tag="VN444_08145"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWR21813.1"
                     /translation="MKSGQILRRESDLLVVADQAALAQEAAKRVVAIAEEAVARCGRF
                     TVALAGGSTPKRLYSLLAAPPA"
BASE COUNT          465 a          723 c          620 g          453 t
ORIGIN      
        1 cgcgtatccc ggtcgaggtg gactatggct ccgagttccg ataccgcaac ccgatcctga
       61 atgagcggac gctggtcgtc acaatcagtc agtcaggtga aacgctggat accctggtct
      121 ccctgcgcga ggcccgtcgc cacggctgca agtccctcgc catctgcaac gtggtcggct
      181 ctacgctcag ccgcgaaagc gatggcgtcc tgtacaccca cgccggcccg gagatcggcg
      241 tcgcctcgac gaaggccttc acctcccaac tcgccgctct ctacctgctg gcattggctc
      301 ttggtcgagc cagagggtgc ctcgatggaa cgagatccca ggaattgctc agtgatctga
      361 tccgcctgcc acagcagatg gagcaggtcc tctcccttgg gcccgcgctg gaagaactgg
      421 ccgggcactt ctacaccgcc tcggatttcc tctaccttgg ccgcggcatc aactacccta
      481 tcgcgttgga gggggccttg aagctcaagg agatctccta catccacgcc gaggggtatc
      541 cggccggcga gatgaaacac ggccctattg ccatgattac tgaagagatg ccagtggtct
      601 ttctcgcgcc aaaggatagg acctttgaga aggtgctgag caatatcgag gaagtgaaaa
      661 cgcgtaacgg gatcgtcatc gccctctccg acgagaccga ttcccgcctg atggccaagg
      721 ccgaccatct ggtcaacatc cctcgcacct cccctcacct gatgccccta ctccttgtcg
      781 tccccctgca actcctcgcc taccacatcg ccgtgcggaa ggggtgtgac gtggaccagc
      841 cccgcaacct cgccaagagc gtgacggtcg agtaggccac atactatccc ggcaggacag
      901 gctccgctgc cctcgaagtt ccgcaacccc acgtctgcca gcatacctgg acgatctgtg
      961 gaggtaacat cgttagaaca cctcaaaata ttctacgata atctggccaa ctttgtgata
     1021 atgcgcatcc gcagtcaaga gacgacaccc gtactgcatg gcgctggcgg cgatccatat
     1081 atcgttcgtg ggtatagggg tcccggcctt ccagaggtta gtgaggatca gtgcgtacct
     1141 ttcggcggta tcctcatcga gaggtaccag gctgacacgc ggcgatgcga gaaattgctg
     1201 aagctccaat cgattctctt ttctgtgctt acctcgcgta aaccccacca gtaactcgcc
     1261 gagaatcacc gcattcaaat agatctcatc gacccgctgg agggcccgtt taacctccac
     1321 atgaccccgc atgaacgcgg agtaggccga ggtatctaac atggctcgcg tcaccgccat
     1381 agatccggat caatggtgcg ctgcttggct aaggctttat tgaaggctgc ggtctcttgc
     1441 tgcgtccacg atccagccag agcgtccaga tcatggtaca gagccgtctc cccagacttc
     1501 cctctaaccc ccacactctg ctccaacaga ccgataacga ccttattgag actggcgtgc
     1561 tgctcatcgg cctttttgcg gatgatccgg gcaagctcag atggaaggtt gcgtaatgtg
     1621 actgccttca tcatcacctc ctattacgct gcacatgatg catcataatg atgcaattat
     1681 agtggcattg atctgaccct gtcaagcctc atgcaagacc tgtgtcatgc accgcgtcct
     1741 gtacacgcac gccggctagg gagatcggcg tcacttcgac aaaggtcgtc acttcccaac
     1801 ctgatgcctc tgcttctcgt cgttccactg taactcctcg cttaccacat cgccgtgcgg
     1861 aaggggtgcg acgtggacca gccgcgcaac ctcgccaaga gcgtgacggt cgaataaccg
     1921 aaggaacacc tcatacggca tagccgcacc acggcgcgca aaaaggcatc ttcaccctca
     1981 tcctctcctc ccccctcgga gggggaggat acaggtgggg ggggattttc gaaggaaggc
     2041 ggaagtcgtg aggtcccgga atcgatgaaa agcgggcaga tactgaggcg cgagagcgac
     2101 ctgctggtgg tggccgatca ggccgccctc gctcaggagg ccgcgaagcg ggtcgtcgcg
     2161 atcgccgagg aggcggtggc ccgctgtggc cgcttcaccg ttgccctggc aggcggctcg
     2221 actccgaagc gcctgtactc acttctggcg gcaccgcccg c
//
LOCUS       DATMXI010000420         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_97151, whole genome shotgun sequence.
ACCESSION   DATMXI010000420 DATMXI010000000
VERSION     DATMXI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97151"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VN444_08150"
     CDS             complement(<1..834)
                     /locus_tag="VN444_08150"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWR21814.1"
                     /translation="MPKSLVIVESPAKAKTIGKYLGKEFVVTASMGHVRDLPAKSLSV
                     DVEHDFAPQYEIIAGREKVVSALKKAAKTADAIYLAADPDREGEAICWHLQQELGTTK
                     KPVHRVTFNEITKRAVQEAFNHPSIIDQRLVDAQQTRRILDRLVGYQISPILWDKVKR
                     GISAGRVQSVALRLIVDRDREIRAFVKAEYWSVDVSLEGRTPPPFVARLHKIDGERVN
                     LPNQEQTDQIVRELPLQEFRVADLTRKEKRRNPVPPSTTSKLQQESVRKLRFTARRAM
                     QI"
     gene            complement(928..2079)
                     /gene="dprA"
                     /locus_tag="VN444_08155"
     CDS             complement(928..2079)
                     /gene="dprA"
                     /locus_tag="VN444_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWR21815.1"
                     /translation="MAGTEQRPVERNAWLALGLIPEVGAATFHRLVQGLGSAEAVLTA
                     KAGALEQVPGISQQVARAIVSFPWRDTLDRELRVIEARGLGLIRFGDEGYPELLAAIH
                     SPPPILYVRGTIEPKDRVAVAIVGSRQASQYGSAMAEQISGELAERGVTIVSGMARGI
                     DAAAHRSALRAAGRTIAVLGCGLGVTYPPEHAELADQIAEQGALISEFPIFTPPKPGH
                     FPQRNRIISGLARGIVVIEAGLKSGALITANYALEQGREVFAVPGQVTSRSSRGCHQL
                     IKAGAKLTEGWEDIWEEIELQTAPGAQVVRDLTPVSRSLAQEEILIIDALEAGPMQID
                     DLIGRTRLPAGQMASLLLSLMLKGMIEELPGKSFAKRLRPLKNRVEGLG"
BASE COUNT          450 a          773 c          665 g          481 t
ORIGIN      
        1 gatctgcatc gcccgcctgg ccgtgaagcg aagctttcgg acggattcct gctgcagctt
       61 actggtcgta gatggcggga ccgggttacg acgcttctct ttcctggtca ggtccgctac
      121 ccggaactcc tgtagcggca gctcccggac gatctggtcg gtctgctcct ggttcggcag
      181 gttcacccgt tcgccgtcga tcttatggag ccttgccacg aacggcggag gggtccggcc
      241 ttccagggag acatcgaccg accagtactc ggctttgacg aacgccctga tctctcgatc
      301 tcgatcaaca atgagacgaa gggctaccga ctgaactcgg ccggcagaaa tgccgcgctt
      361 caccttgtcc cagaggatcg gactaatctg gtagccaacc agacgatcca gaatccttct
      421 ggtctgttgg gcatccacca gccgctggtc tattatggac ggatggttga acgcctcctg
      481 caccgcgcgc ttcgtaatct cgttgaaggt cacgcggtgg acaggttttt tggtagtgcc
      541 gagttcctgc tggaggtgcc agcagatggc ctctccctcc cgatccggat cggccgcaag
      601 atagatggcg tcagccgtct tggccgcctt cttcagggcc gacacgacct tctctctccc
      661 tgcgatgatc tcgtactgcg gggcgaagtc gtgctctaca tcaacgctca gcgacttggc
      721 gggaagatcg cggacatggc ccatcgaggc cgtcacgaca aactccttcc cgagatattt
      781 accgatcgtc ttagccttcg caggcgactc gacaatcact aatgactttg gcacgctctg
      841 ttgttcctcc ctcaaaggct agggtatagg gtttagggta aaagattacc agggggcgac
      901 cctttgaccc tctctaccct ataccctcta ccctaaaccc tctaccctgt tcttcaacgg
      961 ccgaagccgc ttcgcaaaac ttttccctgg tagctcctca atcatgccct tcagcatcaa
     1021 tgacagcaac agtgaggcca tctgcccggc cggaagccga gttcgaccaa tcaggtcatc
     1081 gatctgcatg ggccctgcct ccagggcatc gatgatcagg atctcttcct gcgcaaggga
     1141 acggctgaca ggggtcagat ctcgcacaac ctgcgctcca ggagccgtct gtaactcaat
     1201 ctcctcccag atatcctccc agccttccgt cagtttcgcg cctgccttga taagctggtg
     1261 acacccgcga ctggatcggg atgtcacctg ccctgggaca gcgaagactt cgcgcccctg
     1321 ctccagcgcg taattcgccg tgatcagcgc cccgctcttc agtccggctt caatcaccac
     1381 gatgccgcgc gccaggccac tgatgatccg gttacgctgc gggaagtgac caggcttggg
     1441 cggcgtgaag atgggaaact cgctgatcag ggcgccctgt tcggcgatct ggtcggcgag
     1501 ttctgcgtgc tctggaggat aggtcacccc aagtccgcat cccagcactg cgatggtccg
     1561 gccggcagcg cgcagcgcgc tacgatgcgc tgccgcatcg atcccgcgag ccattccgct
     1621 cacgatcgtc accccgcgct ctgcgagctc cccgctgatt tgctcggcca ttgcgctacc
     1681 gtactggctg gcctgtctgg aaccaacaat cgcgacggcc acccgatctt ttggttcaat
     1741 tgtaccccgc acatagagaa tcggtggggg agaatggatg gctgccagca actcgggata
     1801 tccctcgtca ccgaaccgta tcagaccgag acctcttgcc tcgattactc gcagctcccg
     1861 atcaagggta tcccgccagg gaaaggagac gatagctcgc gcgacctgct gactgatacc
     1921 gggaacctgc tcgagcgccc cggccttcgc cgtgagcacg gcctcggctg acccgagccc
     1981 ctgcaccaag cggtgaaacg tcgccgcgcc aacctcaggg atcaggccga gcgccaacca
     2041 ggcattacgc tctacgggtc gttgttcagt cccggccatg actcgcactg tctcccattg
     2101 ggtcgctggc tgctaagggg atagaattga cgcgtatgca gacatcaggg agatagaccg
     2161 acccagacct tggaactgcc ggcggcaagt ctccaacagg gaaggcggaa aaaggcggga
     2221 tgcatagtag ttgtaacagg ggctggcctc agcccccttc gccctttggc gtatcggtgg
     2281 ggcccccatc ggaaatcccg aggctggacg cccgcccgcc gagagctgca aaaccgtact
     2341 ccagcagcgc tgcggcctcg gtccaccga
//
LOCUS       DATMXI010000421         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_283827, whole genome shotgun sequence.
ACCESSION   DATMXI010000421 DATMXI010000000
VERSION     DATMXI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283827"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VN444_08160"
     CDS             <1..640
                     /locus_tag="VN444_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21816.1"
                     /translation="SWNGERYVEGQPLILPVQVKNLYEFAPIYASGDQISEVAVISEQ
                     RVKAYGSDGKPSWEGKDDLGEVEHLAFSHIPTHRALLARDGLKSGLITPEEHAALQIL
                     PRRVLVETPPLWGNAKTELLTFTNPKKIGLFRIESSLPSQVVAFDRREGTFARGWETI
                     PIEGKVRDVALADLNGAGRKDLIVLSAVKEKGLVAYMQDGSRGIISVFSFTR"
     gene            861..2693
                     /locus_tag="VN444_08165"
     CDS             861..2693
                     /locus_tag="VN444_08165"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWR21817.1"
                     /translation="MSVKRCTSLIGGLLILVGSLAATAPMVSANEELLKLQKDDGQWA
                     SQSKNYAATRYSSLNQITPANVKNLKVAWSFSLGTLNGQEGGPLVVGDTMYVHSSYST
                     GNSHNIFALDLSKEGAPIKWKYVAKHDPRAVPVACCDLVHRGLQYADGKILYQTLDGI
                     VIALDAKTGKELWKTRNADPSKGETSTGAGMVIHDKYIVGVAGGEFGVRGWVAAYDIN
                     TGKQVWKARSAGPDEDLKLASDFNAANPHYGRFGEGTKSWPGEQWKTGGGTTWGWYSY
                     DSELNLLYYSTGNPGTWNPAPRKGGDNKWSMTIWARNPDTGMAKWAYQMTPWDNWDYD
                     GINESILTEQTIGGKKLKVLTHFDRNGFVYTLDRTNGTLLKADAFVYVNWAKGIDLKT
                     GRPIVNEEKLTKQGTDTKNICPCAMGGKNQVPAAYSPVTNLFYASVNNMCMNYEGQLV
                     QYTAGAPYVGASVLMFNGGPEGKDNWWGDVIAWDVVNGKKVWDIKEQQPPWSGPVVTA
                     SNVVFYGTMDGWFKAVDATSGKLLWKHKVGSGIIGNPMTYKGPDGKQYVAVYSGVGGW
                     FGATVSLDLPPDDPTAALGGVNAAYLSGLPMATSKGGTLYVFGL"
     gene            2884..3771
                     /locus_tag="VN444_08170"
     CDS             2884..3771
                     /locus_tag="VN444_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWR21818.1"
                     /translation="MSRRSAILSGFIVVMILFPFSWQAWGDDLKQLQVCGDPDNLPFS
                     NKKSEGFENKIAQVIAKELGAELTYFWWPHQRGLVRRAVRPGLCDVMISIPQGWDQVL
                     WTKPYYRSAYVIVYPKDRGFQITSLDDPILKRLKIGVYINSPPAEALANRDIRTNVVG
                     YSLFYDSGNERPDRMIQDLIAGKIDVVMDWGPMAGYFAKQLNGSSALEVVLLQGGEPG
                     SPFTFEFSMGVRKGNSVLKTELEQAISKRHAEIRKILEDYGIPLLPLLTHEQFSAVEE
                     KPGEVFYRRFDRDDPLTSY"
BASE COUNT          893 a          964 c         1167 g          806 t
ORIGIN      
        1 gtcatggaat ggcgagcggt atgtcgaagg gcagccgctc atccttccgg tccaggtgaa
       61 gaatctctac gaatttgcgc caatctatgc ctctggcgat cagatatcag aggttgctgt
      121 catcagtgag cagcgggtca aggcctacgg aagcgatggc aagccgtcct gggaaggcaa
      181 agatgacttg ggcgaggtcg agcaccttgc cttttcccac atcccaacgc accgggctct
      241 cctggctaga gacgggctca agtcgggcct gattactcct gaagagcacg ccgccttgca
      301 gatcctgccg cggcgggtcc ttgtcgagac gccgcccctg tggggtaacg ccaagacaga
      361 gcttctgacc tttaccaatc cgaaaaagat tgggcttttt cggattgagt cgtccctacc
      421 tagccaggtt gtggcgtttg accgtcgaga agggaccttt gcgcgggggt gggagacgat
      481 ccctattgag gggaaggtgc gtgacgttgc cttggccgat ctgaacggtg ccggcaggaa
      541 ggacctgatt gtgctatcgg ctgtcaaaga aaaagggttg gtcgcgtata tgcaggacgg
      601 gtcgcgtggt atcatcagcg tgttttcttt cacccggtaa gatgtcgcat tgcagagcgt
      661 tgagcgattt atccaatcgt gaagaggcga ggcaaaagaa aggaggagaa agacagctct
      721 gcatctttgc ttcaatgagt gtgtggatcg caatgcctga aaaggcggcg taagtgaagt
      781 aggggcaata ggggtatgag agagaagtgt gagatgattt cctgggaagc aacaggaccg
      841 tatgaagagg gaggcattca atgagcgtga aacgatgtac gtcactgatc gggggtctac
      901 tgatcctggt tgggagcctt gcagcgacgg cgcctatggt ctccgccaac gaggagttgt
      961 tgaaactgca gaaagacgac ggccaatggg cgagccagag taagaactat gcggcgaccc
     1021 gttacagcag cctgaaccag attacgcccg ctaacgtgaa gaacctgaag gttgcgtggt
     1081 cgttctcgtt ggggacatta aacggccagg aggggggccc gctggttgtc ggcgacacga
     1141 tgtatgtcca cagctcctat tctacaggga actcgcacaa catctttgcg ctcgacctgt
     1201 caaaggaagg ggcgccgatc aagtggaagt atgtggctaa acacgatccg cgggcagttc
     1261 cggtcgcctg ctgcgacctc gtccatcggg gcctccaata tgcggacggc aagatcctgt
     1321 accagacgct cgatggaatc gtgattgccc tcgatgccaa gaccggcaag gaactctgga
     1381 agactcgaaa tgccgatcca tccaagggcg agacgagtac cggcgccggc atggtgattc
     1441 acgataaata catcgtcggt gtcgccggag gcgaattcgg agtccgcggc tgggtggcgg
     1501 cctacgacat caacaccggc aagcaggtct ggaaggcccg cagcgcggga cccgatgagg
     1561 atctcaagct cgcgtccgac ttcaacgctg ccaacccgca ttacggacga tttggcgagg
     1621 ggaccaaaag ctggcccggc gagcagtgga aaacgggtgg tggcaccacc tggggatggt
     1681 attcatacga ctctgagttg aaccttcttt attacagtac cggcaaccct gggacctgga
     1741 accccgcccc tcggaaaggc ggagacaaca agtggtcgat gaccatttgg gctcgtaatc
     1801 ctgacaccgg aatggccaag tgggcctacc agatgacccc gtgggataac tgggactacg
     1861 atgggatcaa tgagtccatc ctgaccgagc agacgatcgg tggcaagaag ctcaaggttc
     1921 tgactcattt cgacaggaat ggtttcgtct acacgcttga ccgcaccaac gggacactgc
     1981 tcaaggccga cgcgttcgtc tatgtcaact gggcgaaagg gatcgacctg aagacaggcc
     2041 ggccgattgt gaacgaagag aagttaacca agcagggcac agacaccaag aatatttgcc
     2101 cctgcgcaat gggcggcaag aaccaggtgc ctgccgccta ctccccggtg acgaacctct
     2161 tctacgcttc cgtcaataac atgtgcatga actatgaggg ccagctcgtc cagtacacgg
     2221 ctggggcccc gtacgtgggc gccagcgtcc tgatgttcaa tggcggtccc gaaggcaaag
     2281 acaactggtg gggtgatgtt atcgcctggg atgtcgtcaa tggaaaaaag gtatgggaca
     2341 tcaaagagca gcaacccccc tggagcggac cagtcgtgac tgcgtccaat gtggtcttct
     2401 acggcacgat ggacggctgg ttcaaggcgg tcgatgccac tagtgggaag ctcctgtgga
     2461 aacacaaggt tggatccgga atcattggta atccgatgac ctacaagggg cctgacggta
     2521 agcagtatgt cgctgtgtac tccggggtcg gcgggtggtt tggcgctact gtgtcgcttg
     2581 accttccgcc ggacgatccg accgccgcgt tgggcggggt gaatgctgcc tacctgtccg
     2641 gcctgcctat ggcgacgagc aagggcggga cgctctacgt ctttggcctt taagcgagta
     2701 tgttagctac cggcgccgaa gaattgtatc ttcggcgccg gttttttgcc tataatatgt
     2761 aagtggtcag ctttcagttc gcgaggaaag acgggtagat agactgaaga ccgaagacta
     2821 taaagaagga ttacctgacg accttcagtc ttcagcctaa gcccctattt ctgaggaagt
     2881 accatgagcc gaagatcagc tattctcagt ggattcattg ttgtgatgat cctgtttccc
     2941 ttttcctggc aagcctgggg ggatgatctc aaacaactcc aggtctgtgg cgacccggat
     3001 aacctgccct tttccaacaa gaaatcagaa gggtttgaga ataagatcgc tcaggtaatc
     3061 gccaaggaat taggggctga gctgacctat ttctggtggc cacatcagcg ggggttagtt
     3121 cggagagcgg tgcgtcctgg actctgtgat gtcatgatct cgataccgca gggctgggat
     3181 caggtcctct ggaccaaacc atattaccgg tcggcgtacg tcattgtcta tcccaaggat
     3241 cgggggtttc agatcacatc gctcgacgac ccgatcttga agcgtctcaa gatcggggtc
     3301 tatattaatt ccccaccggc ggaggcattg gccaataggg atatcaggac aaacgtcgtc
     3361 ggctacagcc tcttttatga ctccgggaat gagcgtccgg acaggatgat tcaggacctt
     3421 attgccggca agatcgatgt cgtgatggat tgggggccaa tggccggtta ttttgcaaaa
     3481 cagttgaatg gatcgtcagc tcttgaggta gtcctcctcc agggcgggga gccggggagc
     3541 ccttttacct ttgagttctc catgggggta aggaaaggta attccgtact caagactgaa
     3601 ctggagcagg cgatcagtaa acggcatgcc gagatcagaa agatcctgga ggattatggt
     3661 ataccccttc taccactcct gacgcacgag cagttttcag cagtggaaga gaagccgggg
     3721 gaggtcttct accgtcgttt cgatcgggac gatcccctta cctcttatta agaccgggcg
     3781 aaaggggcca accttaccta aacacgaacg aggaacagtg actatgagac
//
LOCUS       DATMXI010000422         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_271578, whole genome shotgun sequence.
ACCESSION   DATMXI010000422 DATMXI010000000
VERSION     DATMXI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271578"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VN444_08175"
     CDS             complement(<1..345)
                     /locus_tag="VN444_08175"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWR21819.1"
                     /translation="MTAIALVFPGQGSQRVGMGQDFWSQVPEARVLFEKGSAALGIDL
                     AQLCFEGPDERLTLTANAQPAIMTVSMAAFAALQREGIKPDYVAGHSLGEYSALVASG
                     SFAFEDAIRVVRK"
     gene            complement(365..1348)
                     /locus_tag="VN444_08180"
     CDS             complement(365..1348)
                     /locus_tag="VN444_08180"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWR21820.1"
                     /translation="MYGSRIAGTGASVPDRVLTNAELEQMVSTSDEWIVTRTGISERR
                     IASDDQATSDLAEGAARQALEAAGVDPHDLDLILVNTVTPDMFFPSTACVLQERLGAS
                     RAAAFDLMAACAGFVYGLSVADAYLRAGVMRNILVIGADTLSKVVDWSDRGTCVLFGD
                     GAGAVVVQRTTADPAILSTHLYSDGSKGRQLIIPGGGSRQPTSQKVVDEKLVTIRMPN
                     GNDVFKTAVRSMEEAAIAALKANGAEVTDVDLFISHQANARIIYAVAERLDLPRERIY
                     MNIDRYGNTSAASIPIAMDEAVRAGRLKRGDLLLLTAFGGGFTWGSALIRW"
     gene            complement(1365..2363)
                     /gene="plsX"
                     /locus_tag="VN444_08185"
     CDS             complement(1365..2363)
                     /gene="plsX"
                     /locus_tag="VN444_08185"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWR21821.1"
                     /translation="MGTRIALDAMGGDRGPTVTVEGAVVAARELNLSVLLVGNEEEIS
                     QALKQHATKGLSIAIRHAPETVGMQESPSAALRKKKQSSIRVGLELVKNGEADAFISA
                     GNTGAVMATALVTLGPLPGVERPAIALIIPTLKGQSILLDVGANADCKARHLLQFAIM
                     GDVYARQIMGKPSPTVGLLSIGEEESKGNELTREAFKGLEEEQSLNFIGNVEGREVLA
                     GTADIIVCDGFTGNIALKIIEGAAEFFTRLLKEELGKGLAGMAGALLARGAFKRFKKL
                     VDYTEYGGAPLLGVRGVCIICHGRSTAKAIKNAIRVAGECVENRVIEHITEGIAVG"
     gene            complement(2391..2573)
                     /gene="rpmF"
                     /locus_tag="VN444_08190"
     CDS             complement(2391..2573)
                     /gene="rpmF"
                     /locus_tag="VN444_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWR21822.1"
                     /translation="MPLPKRRHSNTRTRTRRSHHALTAPSLSECPNCHERKLPHRACP
                     ACGYYRGREVVKREEV"
     gene            complement(2601..3110)
                     /locus_tag="VN444_08195"
     CDS             complement(2601..3110)
                     /locus_tag="VN444_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWR21823.1"
                     /translation="MVVERSQIPPEGLDLEVREEPCWEGIEGLWLSLAPVQASLHLER
                     EVNGILASGTFSTTAVVPCSRCSEPVAVPVSDSFTILYTEAREAFRDEDVELSAAEID
                     IDTMQDDRLDLTRLLRENVLLNLPLQPLCRVDCRGLCPHCGVNLNESSCGCQVREEDP
                     RLAPLQHLL"
     gene            complement(3201..>3373)
                     /locus_tag="VN444_08200"
     CDS             complement(3201..>3373)
                     /locus_tag="VN444_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWR21824.1"
                     /translation="PHKAAEGTVRRSFAASIERNVVHGSDSAASAAVEIPYFFNRLEI
                     VPAVGRDNSPSI"
BASE COUNT          697 a         1065 c          926 g          685 t
ORIGIN      
        1 cttccttaca acgcgaatag cgtcttcgaa tgcgaagctc ccggatgcta ccagcgctga
       61 atactcccca agcgaatggc cggccacata gtcgggcttg atcccttcgc gctgtaatgc
      121 ggcgaaggcg gccatgctga ccgtcataat agccggctgt gcgtttgcgg tgagcgtcag
      181 tcgctcgtca ggtccctcaa agcagagttg agcgaggtcg atcccgaggg ccgcgctacc
      241 cttctcgaaa agtacacggg cctcagggac ctgcgaccag aaatcctgcc ccataccaac
      301 ccgttgagaa ccttgaccgg gaaaaacgag cgcgatcgcg gtcatcgtcc ggcagcgcgc
      361 atactcacca tcggatgagc gccgatcccc aggtgaatcc acccccgaag gccgtcagca
      421 acaacagatc accgcgcttc agtcgtcccg cccggacggc ctcgtccatg gcgataggga
      481 ttgaggccgc ggaggtgttg ccatagcggt caatgttcat atagatccgc tcccgcggca
      541 ggtccagtcg ttcggccact gcatagataa ttcgggcgtt ggcctggtga gaaataaaga
      601 ggtcaacgtc ggtgacctct gccccattgg ccttgagcgc agctatggca gcctcctcca
      661 ttgaccggac tgccgtcttg aacacgtcat ttccgttggg catccgaatc gttaccagct
      721 tctcatcgac gaccttttgg ctggtaggct gacgcgatcc gccccccgga atgatcagtt
      781 gtctcccctt tgatccgtct gaatacaggt gcgtcgagag gatagccgga tcggcggttg
      841 tccgctgaac taccaccgca cccgcgccgt caccaaagag tacacaggtg cctcgatcgc
      901 tccagtctac gaccttggaa agggtatccg ctcctatcac caggatgttt cgcatgacgc
      961 cggcccgcag ataggcgtcg gcgacagaga ggccgtacac aaagcccgcg cacgcagcca
     1021 tcagatcgaa ggccgccgcg cgtgacgcgc caagtcgctc ctgcaacacg cacgcggtag
     1081 agggaaaaaa catatcgggg gtcaccgtgt tcacgaggat cagatccagg tcatgagggt
     1141 cgacgcctgc ggcctcaagg gcctgtcgag ccgctccctc agcgagatcc gacgttgcct
     1201 ggtcatcgga ggcgatccgc cgctccgata tccccgttcg ggtgacaatc cactcatcgc
     1261 ttgtgctgac catctgctcc agttcagcat tggtcagcac ccgatccgga actgaggccc
     1321 ctgtgccagc gattctgctg ccgtacatca catacgtcct gtcgctaacc gacggcaatc
     1381 ccttccgtga tatgctcgat caccctgttt tcgacacatt ctccggcgac tcggatcgca
     1441 ttcttgatgg ccttggcggt cgagcggccg tggcagatga tacagacccc tcggaccccc
     1501 agcagtggtg cgccaccgta ctccgtgtag tcaaccagct tcttgaagcg cttaaatgcg
     1561 cctcgggcca acaaggcgcc ggccatcccc gccaaccctt tccccagttc ctccttgagc
     1621 aagcgcgtaa aaaactccgc ggcgccttca atgatcttca aggcaatgtt accggtgaag
     1681 ccatcgcaca caataatatc ggctgttccc gcgagaacct cgcggccctc gacgtttccg
     1741 atgaagttga gggactgctc ctcttcgagc cccttgaagg cctctcgagt cagctcgttc
     1801 cccttgctct cctcctcccc gatgctcagc aggccaacgg taggcgaagg cttccccatg
     1861 atttgccgcg catagacatc ccccataata gcaaactgga ggagatgccg cgctttgcaa
     1921 tcggcattgg cgccgacatc caacaggatc gactgacctt tcagcgttgg aatgatgagg
     1981 gcgatggcgg gacgctcaac gcccggaagc ggacccaaag tcaccagcgc ggtggccatc
     2041 actgcccccg tattcccggc gctgataaag gcatcggcct ccccattctt gacgagctcc
     2101 aggccgaccc ggatggacga ctgcttcttc tttcgcaagg cggcagaagg cgattcttgc
     2161 atcccgaccg tctccggggc gtgtcggatc gcgatgctca ggcccttcgt ggcgtgctgt
     2221 ttcagtgcct ggctgatttc ctcttcgttc ccgaccagca agacagacag gttgagttca
     2281 cgggcggcga ctacggcgcc ctcaacggta accgtgggac cacggtcgcc acccatcgcg
     2341 tcaagggcta tacgggttcc catacctgtg tgcctatcgg gagcgggagg ctagacctcc
     2401 tcccgcttca caacctcccg accacgataa tagccacagg cggggcaagc gcgatgaggc
     2461 agcttccgct catggcagtt ggggcactcc gaaagagaag gtgcggtgag ggcgtgatga
     2521 gagcgtcgtg ttcgtgtccg cgtgttactg tgacgacgtt ttggaagggg catcgttcat
     2581 cctcctggac ttcacgtggg ctagagtaaa tgttgaaggg gagccagcct tgggtcctct
     2641 tcccgaacct ggcacccgca cgagctctcg ttcaggttga ccccgcaatg gggacaaaga
     2701 ccacggcagt cgactcggca aaggggctgc agcggcaaat tcagcaggac attctcgcgc
     2761 aacaacctgg tcaaatccag gcgatcgtct tgcatggtat cgatgtctat ctcggcggcg
     2821 ctgagttcaa cgtcctcgtc gcgaaaggct tctctggctt ccgtatacag aatcgtaaac
     2881 gaatcagaga cgggaacagc aaccggctct gagcatcggc tgcacggcac gactgccgtt
     2941 gttgagaatg ttccactcgc aagaataccg ttgacctctc gctccagatg aagggaggct
     3001 tggaccgggg caagtgacag ccataatcct tcgatcccct cccagcaagg ttcttcacgc
     3061 acctccagat ctaacccttc tggaggaatc tgtgaccgct ccactaccat tgtagatgct
     3121 ccatcaaatt cgcacgtatt ttacaaggca aggactctca ggtcaaggaa tttcctgacg
     3181 ggacagccct cgcttccagc ttatatcgac ggtgaattat ctcttccgac tgcaggaacg
     3241 atctctaatc gattaaaaaa atacgggatt tcaacagcgg ctgaagccgc cgagtctgag
     3301 ccatgcacga cgttcctctc gatgctggcg gcaaaactcc gtcggactgt cccctcggca
     3361 gccttgtgtg gat
//
LOCUS       DATMXI010000423         4478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U13116
            k141_37980, whole genome shotgun sequence.
ACCESSION   DATMXI010000423 DATMXI010000000
VERSION     DATMXI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943713
            Sequence Read Archive: SRR7629277
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 22:08:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,647
            CDSs (total)                      :: 1,625
            Genes (coding)                    :: 1,606
            CDSs (with protein)               :: 1,606
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 12 of 19
            Pseudo Genes (internal stop)      :: 6 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37980"
                     /isolate="SMAG_U13116"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.09 N 93.13 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VN444_08205"
     CDS             complement(<1..296)
                     /locus_tag="VN444_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010642498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWR21825.1"
                     /translation="MTTYKLAPSILSADFARLGEEVRAVDAAGADYIHVDVMDNHFVP
                     NLTIGPAVVAAIRPHTAKPMDVHLMIEPVDAIIPDFAKSGADIITVHPEATRHL"
     gene            complement(327..1391)
                     /gene="fba"
                     /locus_tag="VN444_08210"
     CDS             complement(327..1391)
                     /gene="fba"
                     /locus_tag="VN444_08210"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942862.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWR21826.1"
                     /translation="MALISMRQLLDHAAEHGYGVPAFNVNNMEQIQAIMEAAHEVDSP
                     VIMQASAGARKYASEPFLRHLFLAALEMYPDIPVVIHQDHGASPGVCIASIRSGFSSV
                     MMDGSLFADGKTPSTYEYNVATTAHIAEVAHAIGVSVEGELGCLGSLETGTGEKEDSH
                     GAEGALSRDQLLTDPEQAAQFVKATKVDALAIAIGTSHGAYKFSKKPEGDVLVMERVK
                     EIHARIPDTHLVMHGSSSVPQEWLKIIREFGGDMPQTYGVPIEEIQLGIKHGVRKVNI
                     DTDLRLAATGAIRQDLAQGKKNFDPRKFLMAATKAMRQICKDRYEQLGSAGNASKIRS
                     ISLDDMAHRYAKGELDPHVN"
     gene            complement(1431..2432)
                     /gene="glpX"
                     /locus_tag="VN444_08215"
     CDS             complement(1431..2432)
                     /gene="glpX"
                     /locus_tag="VN444_08215"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HWR21827.1"
                     /translation="MSSVLTMNKCLGRDLALDLVRVTEAAALAAGRWMGRGQKDAGDE
                     AAVHAMRAMLGTVDIDGVVVIGEGEKDEAPMLFNGEAVGTGQGPAVDIAVDPVEGTSL
                     LAHGRPDSIAVIGVAPRGTMWSPGPGFYMNKLVVGREAREAISPTSLALPVAELLAAI
                     GKAKHKAVADLTVFVLDKPRHIGLIADVRAAGARVLTRTDGDVAGALMAATPGSGVDV
                     LMGIGGTPEGVISACAVRALGGAMLGQLAPQKPGEREALLAAGLDIDRVLTECDLVAS
                     DDVFFSATGITDGLLMQGVRYADGGSTTHSIVMRGKSRTRRTIQAEHCVDKVRTIGS"
     gene            complement(2716..2940)
                     /locus_tag="VN444_08220"
     CDS             complement(2716..2940)
                     /locus_tag="VN444_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HWR21828.1"
                     /translation="MFLKDKQAGHLVEVLDLCALFDPLQSVVKGRIHVGEEMQDPASF
                     DKSDLIFPSGECLPRYWVDVHYRDAAGTIG"
     gene            complement(2974..3525)
                     /locus_tag="VN444_08225"
     CDS             complement(2974..3525)
                     /locus_tag="VN444_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21829.1"
                     /translation="MPEAEAETTYVDNIFGSVTDERVVYHPNRGWLTEGREENAPLSH
                     IASVEFVISRSVSNGILLILIGVPTMLVLIGVMFILFGYCLLKGTPTVIVNTTDGNRE
                     IMKGWPWHREQAYDFAKALQSRIRPDQEVRLEYVSSVSVGTSRHFLNRPLLFVAVLAL
                     LALVIWAIVWLGMLFGLTPFSSL"
     gene            complement(3878..4162)
                     /locus_tag="VN444_08230"
     CDS             complement(3878..4162)
                     /locus_tag="VN444_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HWR21830.1"
                     /translation="MPKYLIERDIPGAGQLSAKELQAISQKSCGVLGKLGPQIQWVHS
                     YVTDEKVYCVYIAPNAEMVLEHARHGGFPANRVSEIRSVIDPTTAEGGSC"
     gene            complement(4282..>4478)
                     /locus_tag="VN444_08235"
     CDS             complement(4282..>4478)
                     /locus_tag="VN444_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR21831.1"
                     /translation="RTLTIIKQIPVGQRAWYLAFTPDEQRLYVACGRSDSVSVIDVAT
                     EKVIATVPVGKMPFGVAIPK"
BASE COUNT          970 a         1370 c         1229 g          909 t
ORIGIN      
        1 tccagatggc gggtcgcctc cggatgaacg gtaatgatgt cggccccgct cttggcgaaa
       61 tctggaatga tggcgtctac cggttcgatc atcaggtgga catccatcgg ctttgccgtg
      121 tggggacgga tcgcggcgac cacggccggc ccaatagtga gattgggcac aaagtgattg
      181 tccatcacat ccacgtggat atagtcggcg ccggccgcat ccaccgcccg gacctcttca
      241 cccagccggg caaagtcggc agacaggatc gaaggggcaa gcttataagt agtcacaggg
      301 tctcgcacgg ccatcacgag gccggactag ttgacgtgcg gatcgagttc gcccttggcg
      361 tagcgatggg ccatatcgtc cagagagatc gaccggatct tgcttgcgtt gccggcgctc
      421 cccaactgtt catagcggtc tttacagatt tgccgcatgg ccttcgtggc cgccatcagg
      481 aacttgcgcg gatcaaagtt ctttttgcct tgcgccagat cctgccggat ggcgccggtt
      541 gccgccaggc gcagatcggt atcgatgttc accttccgca ccccatgctt gatcccaagc
      601 tggatctctt cgatcggcac cccgtaggtc tgcggcatgt cgccaccgaa ctctctgatg
      661 atcttcagcc actcctgggg aacgcttgag gatccgtgca tgaccaggtg ggtgtccgga
      721 atgcgcgcat ggatctcctt gacccgctcc atcacaagga catcgccttc aggcttcttg
      781 gagaatttat aggcgccgtg gctggtgccg atggcaatag cgagggcatc caccttggtc
      841 gccttgacga actgggcggc ctgctctggg tcggtcagga gctgatcccg tgagagcgcc
      901 ccctcggcgc cgtggctgtc ttccttctcg ccggtgccgg tttcgaggga ccccaggcag
      961 ccgagctccc cttccactga cacgccgatc gcgtgggcaa cctcagcgat atgagccgtt
     1021 gtcgcgacat tatactcata cgttgaaggg gtcttaccgt ccgcgaagag cgagccgtcc
     1081 atcatgacgc tgctgaagcc ggagcggata gacgcgatac acaccccagg ggatgcgccg
     1141 tgatcctggt gaataaccac cgggatgtca gggtacatct ctagtgccgc caggaacagg
     1201 tggcggagga acggttcgct ggcgtacttg cgcgcgccgg cggaggcctg catgatcaca
     1261 ggactgtcca cctcatgagc cgcctccatg atggcctgga tctgttccat gttgttcacg
     1321 ttaaaggcgg ggacgccata accatgctcg gcagcgtggt cgagtaactg tcgcatcgaa
     1381 atgagtgcca tgccttcctc ctgtaccttg atatatgtcc cgtacctgtc ctaagatccg
     1441 atggtcctca ctttgtctac gcaatgctct gcctgaatcg tgcgcctggt tcggctctta
     1501 ccacgcataa cgatcgagtg ggtcgtcgag ccaccgtctg cgtatcgtac gccttgcatc
     1561 agcaggccat cagtaattcc agtagccgag aagaacacgt catcgctggc tacaaggtcg
     1621 cactcggtga gcacccgatc gatatcgaga ccggcggcga gcaacgcctc acgttcgccg
     1681 ggtttctgag gggctagttg gcccagcatc gccccgccca gcgcgcgaac agcacaggcg
     1741 gagatgacgc cttccggtgt cccgccgatg cccatcagga catccacccc ggagcctggc
     1801 gtagcagcca tcagcgcgcc ggccacgtcg ccatcggtac gcgtcagcac gcgcgcgccg
     1861 gcggcccgca catcggcgat gagcccgata tgtcggggtt tgtcgagtac gaatactgtc
     1921 aggtctgcca cagctttatg cttcgcctta ccgatggccg cgagtaactc agccacgggc
     1981 agagcgagac tagtcggaga gatagcctcc cgcgcctcac gcccgaccac cagtttattc
     2041 atgtaaaagc cagggccagg ggaccacatc gtgccccgcg gcgccacacc gatcacggca
     2101 atcgaatcag gccggccatg ggccagtaga ctggtgccct ccaccgggtc caccgctatg
     2161 tccacggctg gtccttggcc ggttccaacc gcctcgccat tgaagagcat gggcgcctcg
     2221 tctttctcgc cttcgccgat gacgaccaca ccgtcgatat cgaccgtccc gagcattgcc
     2281 cgcatggcat gaacggccgc ctcgtcgccc gcgtccttct gcccgcgccc catccaccgg
     2341 ccggcagcca acgcggcagc ctcggtcacg cggaccagat caagcgcgag gtcacgcccc
     2401 aggcacttat tcatcgtcaa tacgctgctc ataggtatcc cctctatgat gtgagaactc
     2461 accgaaggct ggattcaaac aaaaacgcca tcagagatca aggcagacct aagaaggcgt
     2521 gttgttaggg gagaaatatt tcatacgcgc tctgcaatgt caactttttc tcgcgtctct
     2581 cttcgactac gccagcaagg tggcagcctt tgcgtacgag aaaccttttc ggagctaagg
     2641 agcgatcact gcagcattcg acgcgataga ccagatcgac caaacagacg aaagagacat
     2701 attctgagga acgggctatc cgatggtgcc ggcggcgtct ctgtagtgca catccaccca
     2761 gtagcgcggc aggcactcac cagagggaaa gatcagatcg gacttatcaa aggaggcagg
     2821 atcttgcatc tcctctccga catggattcg gcccttcaca actgactgaa gtggatcaaa
     2881 cagtgcacaa aggtcaagga cctctaccag gtgaccggcc tgcttatctt tcagaaacat
     2941 ttcagatcct ccttacatca tgagtatcat ccgctatagt gaagaaaagg gcgttaggcc
     3001 aaacagcatg cccagccaga cgatggccca gatgacaaga gccaacagcg ccaacacggc
     3061 cacaaaaagg agaggtctgt tgagaaaatg gcgcgatgtc ccgaccgaaa ccgaactgac
     3121 atactcaaga cgaacctcct gatcaggtct aatccggctt tgaagagcct tcgcgaaatc
     3181 ataggcctgt tcgcgatgcc acggccagcc tttcatgatc tctcggttcc catccgtggt
     3241 gttgacaata acagttgggg tacccttgag gaggcagtag ccaaacagga taaacataac
     3301 accgataaga accagcatcg tgggtacccc aatgagaata agaaggatcc cattgctgac
     3361 gctgcgggat attacaaatt ctaccgacgc gatatggctg aggggcgcat tctcctcgcg
     3421 cccttctgtc aaccaacctc tattcgggtg gtagacgacc cgttcgtctg tgaccgaccc
     3481 gaatatgttg tcgacgtagg tcgtttctgc ttccgcctcg ggcatgcctc gacctccatt
     3541 caccctgaca ggaccagcgc cgctctaagc tagttgcgga ctaattgtac gctcgatgga
     3601 ccgatagacc ttcgtaaaac aatccgccac caaccgtcgc tacgcccaca tatcccatga
     3661 agtctatttt cgtagctaga gtccatggag ccacctaacg attgagtaag cttgtcggca
     3721 tagccactct aagatcttct acaactgctg cgattctcca acctttcagg gagcttgtca
     3781 aggttcagaa aatagcgatc agcagtcagc attcagctat cagcctcagg taacagcggg
     3841 agcagatcag aaaaagctga gagctgaccg ctgggagcta acagcttcct ccttcggcgg
     3901 ttgtgggatc tatcaccgac ctgatctccg acacgcggtt cgccgggaag ccgccatggc
     3961 gggcgtgctc caggaccatt tcggcgttcg gcgcgatgta gacacaatag accttctcat
     4021 cggtcacgta gctgtggacc cactgaatct gcggccccag cttgccgagc acgccgcatg
     4081 acttctgcga gatggcctga agctctttgg ccgaaagttg ccccgccccg gggatgtcac
     4141 gctcgatcag atactttggc atcgtcaatc tccttagaaa aagcggtcag ctttcagccg
     4201 tagccgtagg tcgggttagc gcagcgtaac ccgacaacta tcagcctcat gagtagtgtc
     4261 caccacgact atgataagtc atcatttcgg gatcgcgacg ccaaacggca tcttcccaac
     4321 cggaaccgtg gcgatcacct tctcggttgc gacgtcgatg acggaaaccg aatcactgcg
     4381 gccacacgca acatagagac gctgctcgtc cggcgtaaag gccagatacc aagcccgctg
     4441 gcccaccggg atctgtttga tgatggtcaa ggtacgag
//
