LOCUS       DATMXJ010000001         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_52298, whole genome shotgun sequence.
ACCESSION   DATMXJ010000001 DATMXJ010000000
VERSION     DATMXJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52298"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VN377_00005"
     CDS             <1..1133
                     /locus_tag="VN377_00005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HWR26797.1"
                     /translation="DRTADAVCDEAKLDRNLNPADMSREQFLLLHQAFKRVKIMAPPT
                     ECLSPIGETLIRRSLKSETQEISPEFIFTASRPAAVFSGNPFQVEVGIVYGGNLPKDK
                     PVKILRFANRIPLLYQQGDCAVTTAIASIDWRRYGLDQPSGTGIPIGPAIFLTHIAST
                     QIPYTSESKEAIADVEEIENEIRLAFREVARKVQTHINKKVRRVKTREKFDLITKILP
                     EIAKKSAHMLNKPIPSLEKVITRIMDVVWIEDVVEYEKVRGKQVQTKLDITNPKGQTE
                     SGGWIAKSTIMVVNYKGKPQKFNLYALFPKNAVVGEVKPKPTKVTDSYIKWSLESIDP
                     TDKVDLYFELAGLNKGDFDENDLYVQNINPTYVIGADKWEGE"
     gene            1138..2235
                     /locus_tag="VN377_00010"
     CDS             1138..2235
                     /locus_tag="VN377_00010"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HWR26798.1"
                     /translation="MARDYSGVITKIDSIAAKVYADLEKAEIPALQLSTRTKANIRFD
                     DDLNVWKYGKNKTERTANSLDGAYMLLRTMYLVDFIKDMIKEKKSSTLRETYYISEGW
                     NLAKFSSQNESDLLAEDLEVITGCMREDFKLRPEEDGARVIGNLVVEEMTRNNTKKRI
                     HCQDDVGDAGYGIPYNVEKEKLSFKEVDADFIVAIETGGMFDRLVENGFDTDSRAILV
                     HLKGQPARSTRRFIKRLNEEKKLPVLVFTDCDPWSFRIFGSVAYGAIKTAHISEYLAT
                     PAAQYLGVTPSDIENYKLPTDSLTTEDIAALKSELDDPRFQDQFWKHEIKLQLSLGKK
                     SEQQALAKYGLDYVTDTYLPEKLVEMGMLKK"
     gene            complement(2244..2702)
                     /locus_tag="VN377_00015"
     CDS             complement(2244..2702)
                     /locus_tag="VN377_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26799.1"
                     /translation="MAATESSWDEESQKQRLLKTTKFFFLFAVLYCLWIVILIMGVYF
                     LELGNKWAVLTMEQWILSAIALISIAIGLEVVLLLQYMLSRRKRLEPLKPKKQKQKEY
                     IQGKQVHSYTIPLDSKGGIFSKTYILIDEGRVLNLRYQMIPPNDLWGQQQ"
     gene            2871..3107
                     /locus_tag="VN377_00020"
     CDS             2871..3107
                     /locus_tag="VN377_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26800.1"
                     /translation="MNNADIKIVGAVTDENALEEIEDIAKIFAYEQRMKDYTPLLHPP
                     FQAKKVKTIEEKKTGESNKFIESFQPRVLVKGLC"
     gene            3251..3478
                     /locus_tag="VN377_00025"
     CDS             3251..3478
                     /locus_tag="VN377_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26801.1"
                     /translation="MVEFETLKAEEVKFGKNNFIEVARKKAVGESLSKEFVAISRGFY
                     LSDGSKKWKSSITLPDEVDKRKQIATLINDL"
     gene            3948..>5121
                     /locus_tag="VN377_00030"
     CDS             3948..>5121
                     /locus_tag="VN377_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWR26802.1"
                     /translation="MKKSFIALGFCILLLCTPLGYSDNSDCGCTVPDDLSTSSSSAPW
                     ITDADITALQQQAVVEGWTFTVSKNPATDRPLSELCGLVEPDNWWVNASFDPCVPSTT
                     LPSHFDWRELGGCTPVKNQGGCGSCWAFGTIAPLECNILIKDHKEVDLSEQWLVSCNR
                     NGWGCGGGWWAHDYFQWKMDKSNGTGAVLEKDFPYQATDLPCNGPFQHPYLIDSWHYI
                     GFPQGVAPTESIKQAIMTYGPVSVSCAVTQAFGAYDGGVFNEDDPNASINHAIALVGW
                     DDNQGENGVWFLRNSWGPDWGEDGYMRIEYGVCKVGYSACYVNYPVKTKVDISSGLFC
                     VTVGLRNVGNTVTTAIKCNISVNGGILGAINSSLIDTIDSLEPGAVVYERIPCLGFG"
BASE COUNT         1435 a         1050 c         1259 g         1377 t
ORIGIN      
        1 gtgatcgaac agcagatgcg gtctgtgatg aggcgaaact tgatcgaaac cttaacccag
       61 ctgatatgtc tcgcgaacag ttcctccttt tgcatcaggc attcaaacga gtgaaaataa
      121 tggctccgcc aacagaatgc ctttccccga ttggtgagac gcttatccgt cggagcttga
      181 agagcgaaac ccaggagata tcaccagagt ttatttttac cgcttcacga cctgccgcgg
      241 ttttttcagg gaacccgttc caggtggaag tcgggattgt ctatggcggt aatctgccga
      301 aggataaacc agtcaagatt ctccggttcg cgaaccggat tcccttgctg tatcagcagg
      361 gtgattgtgc ggttaccact gcgattgcat cgattgactg gcgtcggtat ggcctcgatc
      421 agccctcagg gacagggatc cctattggtc ctgcgatctt tctaacgcat atcgcttcta
      481 cccagatacc ctatacatcg gagtctaagg aggctatagc tgatgtcgag gagattgaaa
      541 atgaaattag acttgcgttc cgtgaggtcg cacgaaaagt ccagacgcat atcaataaaa
      601 aagtccgccg ggtgaagacc cgggagaaat tcgacctgat cacaaagata ctccctgaga
      661 ttgcgaagaa atccgctcat atgctcaata aaccaatccc atctcttgaa aaagtcatca
      721 ccaggatcat ggatgtggta tggattgagg atgtggtgga gtacgaaaag gtaagaggaa
      781 aacaagtgca gacaaaactg gatataacca atccaaaggg gcaaacagaa tctggcgggt
      841 ggattgcgaa aagcacgatc atggtggtga actataaagg gaagcctcag aagttcaatc
      901 tgtacgctct tttcccgaaa aatgcggtgg tcggcgaggt aaaaccaaaa ccgacgaaag
      961 tcactgatag ttatatcaaa tggagtttgg aaagtatcga tccgaccgat aaggttgacc
     1021 tgtattttga gttggctggt ttgaacaaag gggattttga tgaaaatgat ctgtatgtgc
     1081 agaacatcaa cccgacctat gtgatcgggg ctgataaatg ggaaggtgaa taggaacatg
     1141 gcacgtgatt attctggagt cattacaaag attgattcga tagcggcaaa ggtgtatgct
     1201 gatcttgaaa aagcagagat ccctgcgcta cagctgtcga cacgaacgaa agcgaacatc
     1261 aggtttgatg atgatctcaa tgtctggaaa tatgggaaga acaagacaga gcggactgca
     1321 aactcccttg atggcgcgta catgctgttg cggaccatgt acctggtcga tttcatcaag
     1381 gacatgatca aggagaaaaa gtcatccacg ttaagagaaa cctattatat ctcggagggc
     1441 tggaatcttg cgaaattttc tagtcagaat gaatcggacc tgcttgcgga ggatcttgag
     1501 gtgatcacgg ggtgtatgcg tgaggatttc aaactcagac ctgaggaaga cggtgcccgg
     1561 gtgatcggga acctggttgt cgaggagatg actcgcaata acaccaagaa gaggattcat
     1621 tgtcaggatg atgtcggtga tgctggatac gggattccct ataacgtgga gaaggaaaaa
     1681 ctctccttca aagaggttga tgctgatttt attgtcgcga ttgagaccgg tggtatgttt
     1741 gaccggcttg tggagaacgg ttttgataca gactcccgcg ccatccttgt gcatctcaag
     1801 gggcagcctg ctcggtcgac acggcgattc attaaacggt tgaacgagga aaaaaaactc
     1861 cctgtgctgg tgttcaccga ttgtgacccc tggagtttcc gtatcttcgg gagtgtcgca
     1921 tatggtgcga ttaaaacagc gcatatctca gagtatctcg cgacaccagc agcacagtat
     1981 cttggggtga ctccctcaga tattgagaac tataagctgc cgactgattc gttgaccact
     2041 gaggatatcg ctgcgttgaa aagcgagctg gatgacccgc ggttccagga tcaattctgg
     2101 aaacatgaga tcaaattaca gctcagcctt gggaagaaat cagaacagca ggcattagcg
     2161 aaatacgggc ttgattatgt caccgatacc tatctgcctg agaagctcgt ggaaatgggg
     2221 atgttgaaaa agtaacttgt gcgttattgt tgctgtcccc agaggtcatt tgggggaatc
     2281 atctgataac gcaggttcag cactctgccc tcatcgatga ggatataggt tttactgaaa
     2341 ataccgcctt tcgaatcaag agggatggtg tagctgtgca cctgttttcc ttgaatatat
     2401 tctttctgtt tttgtttttt tggttttagg ggttcgagtc gctttctcct tgaaagcatg
     2461 tactgaagta agagaacgac ctcaaggccg atggcgatgc tgataagggc tattgcagat
     2521 aggatccatt gttccatggt taagactgcc catttgtttc ccagttcgag gaaatagacg
     2581 cccatgataa ggatgacaat ccacagacag tacaggacgg caaataagaa aaagaatttc
     2641 gttgttttta gcaagcgttg cttttgggat tcttcatccc aggaagattc tgttgcagcc
     2701 atttcctacc aattctgtta tgattcattg actttaaaac tttttcatgg tatttctaca
     2761 tgaaaatttt actagctccc tgtaaaagcc tgtttgatgt ttttttcaaa acctttatat
     2821 tcatggactg acaaatagat acatgctagt aaaaatctag cgttaatgag gtgaacaacg
     2881 cggatataaa aattgtgggg gcagtaactg atgaaaacgc gctcgaagaa attgaggaca
     2941 tcgcgaaaat ctttgcctat gagcaaagaa tgaaagatta tactcctctt ttgcatccac
     3001 cgtttcaagc aaaaaaggta aaaacaatcg aggagaaaaa aacaggggag agcaataaat
     3061 ttattgagag ttttcaacca cgtgtcttag taaaaggatt gtgttagagc ccttttcttt
     3121 ccttgcgcga gtctcactgg ggactctctg tttctttttc tcttattttt tactttttcc
     3181 cttcaagttt ttgtttaatt gaaaaagttt aaattcaact aatcttttct tccaggcaaa
     3241 gaggtagaca atggttgaat ttgaaacact gaaagcagag gaagtaaaat ttggaaaaaa
     3301 taatttcatc gaagttgcac ggaagaaagc ggtcggtgaa tcattatcga aagaattcgt
     3361 cgcgatttcc cgcggattct atctttctga tggatcgaag aaatggaaat catctatcac
     3421 gcttcctgac gaagttgata aaagaaagca aatcgcaaca ttaattaatg atttatagaa
     3481 aaaaagggta tacccgcaac tcgtcttacg taaaaccata cctgagttgc cagttctttt
     3541 tcttatttta ttagaagact gcttatttat ctcagacgaa atactcctcg ttttgccatg
     3601 ggaaaacatc aggttttttc cttaaataac agatctgttt cgatttggac tggtgtcgtt
     3661 gaatggaagt ggtttttctt gttgtacatt ttgtggatgg tcaaagaatt aggacagcgt
     3721 ttttgtaaaa aacatggtca taacgtactt ggacccgatt aatgtgtaaa acacttcggc
     3781 gtagaacagt ccaactgcga catcccagct tagtgaatcc cctatttttt aattactcca
     3841 aataactaag aaaaaaatgt ctcactttta aatgcaggat aaaccataga tttattaagg
     3901 agagtctcta ttgaaccaac tgatacgttc gtaaggggac tttcaatatg aagaaatcgt
     3961 tcattgcact tggtttttgt attcttttac tttgtacgcc cttaggctat agtgataaca
     4021 gcgattgtgg atgtacagtt ccagatgatc tctccacatc atcgtccagt gcaccatgga
     4081 ttactgatgc ggatatcacc gcgttacaac aacaggcagt cgtagaaggc tggacgttta
     4141 cggtttccaa aaacccagcg acagatcgtc cactctcgga actctgtggt cttgtcgaac
     4201 cagacaactg gtgggtgaac gcttcgttcg acccctgtgt tccctcgacg accttaccgt
     4261 ctcattttga ctggcgtgag cttggaggtt gcacacctgt gaagaaccaa ggtggatgcg
     4321 ggagctgctg ggcgtttgga acaatagcac cactcgaatg caatatcctc atcaaagacc
     4381 ataaagaagt tgacctttca gaacaatggc tcgtcagctg caaccgaaac ggatggggct
     4441 gtggtggagg atggtgggcc catgattact ttcaatggaa gatggacaag tctaatggaa
     4501 ccggtgcggt cctagagaaa gattttccct accaggcaac ggaccttccc tgtaatggtc
     4561 cgtttcaaca cccgtatctg atcgactctt ggcattacat cggattccct caaggtgtcg
     4621 ccccaacaga atccattaaa caagcgatca tgacctatgg tccggtttcg gtctcctgtg
     4681 cggtgaccca ggcgttcggt gcatacgatg gcggggtctt caatgaagac gaccccaatg
     4741 catcaatcaa ccatgcgatc gcattggtcg gatgggatga caaccaggga gaaaacggtg
     4801 tgtggttttt gcgtaattca tggggacctg attggggcga agacgggtac atgcgtattg
     4861 aatacggggt ctgcaaggtt ggatattcag cctgctatgt gaattatccg gtcaagacta
     4921 aggttgatat ctcaagcggg ttgttctgtg tcaccgtcgg cctacggaac gtgggtaaca
     4981 cggtcacgac cgctatcaaa tgtaacatca gcgtgaatgg cggaatcctt ggagccatca
     5041 attcttcgct cattgacacc atcgactcat tagaaccagg ggctgtggtg tacgaaagga
     5101 tcccttgtct ggggttcggt c
//
LOCUS       DATMXJ010000002        10226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_5242, whole genome shotgun sequence.
ACCESSION   DATMXJ010000002 DATMXJ010000000
VERSION     DATMXJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5242"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..736
                     /locus_tag="VN377_00035"
     CDS             26..736
                     /locus_tag="VN377_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR26803.1"
                     /translation="MILKVSIVIPAVNEERGIGKTIDAVNKEYFNKQHWDLEILLVDG
                     NSKDKTREIATSKGVIVLLEKQKGYGRAYKVGIAHATGDIIVTGDADATYPFHLIHDY
                     IQMLLDRHLDFLTTDRFAALKHGSMSLKHRFGNLTLAWTLRILFLVNIRDSQSGMWIF
                     KRDALTKIQPLNDFNDGMPFSEEIKIEMFTNKHIKAKEIPSTLFAREGQVKLESFKDG
                     IKNLRFLFKKRVTPSKRH"
     gene            790..1134
                     /locus_tag="VN377_00040"
     CDS             790..1134
                     /locus_tag="VN377_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HWR26804.1"
                     /translation="MDPLMTIWVFFIGCFFGVILGVMLSYRTAVTPLQHTISKLTSQD
                     QQYHETMKYYPYDREKFRFIGGPIDGIQFEEDCIIFVRLKEGNIPRSKEQENIKNLLE
                     NGKIQWFEFPTK"
     gene            complement(1217..2365)
                     /locus_tag="VN377_00045"
     CDS             complement(1217..2365)
                     /locus_tag="VN377_00045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWR26805.1"
                     /translation="MKSKADVVIIGGGVNGCSLAYNLAKKDLDVVVVEKKYLSSGATG
                     ACGAGIRQQWSTRENASLAIQSVKIFEQLSRELGQDIEFRQGGYLIAIHDKKELKQAE
                     KNVAMQRSLGLKVDILKPEEITDIVPILDVKGMKAIGATFCPTDGHANPFRTTFAYAD
                     AARNLGAEINTHTTVVNLKTSKKAIHAVVTDKGTIQTDVVVNAAGIDSKTIAEMVNIT
                     LPLAPFRKEIMATERIQPLFEAMVISFKDGIYFSQQKEGQIVGGIPIPEEKGGYKTMP
                     TFSFLEHMSRTLTRYAPVLKHVNLLRHWTGFYDVTPDARPILGEVKEMKGFLQCNGFS
                     GHGFMISPMVSRLLTTLITEGKKSEVLENLTLDRFKGKNIEKELSVVG"
     gene            complement(2355..2627)
                     /locus_tag="VN377_00050"
     CDS             complement(2355..2627)
                     /locus_tag="VN377_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWR26806.1"
                     /translation="MKRSIVCRCEDISEEDVIQAIDKGYTDIEELRKKLRIGMGPCQG
                     RVCIQLVARILERKTGKKVANIPLPTVRPPLIPVSLGTLASEKDEK"
     gene            complement(2631..3092)
                     /locus_tag="VN377_00055"
     CDS             complement(2631..3092)
                     /locus_tag="VN377_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWR26807.1"
                     /translation="MRQYEKTGILSAKDLQIPSEKQLEKGVAILECVQEIPCNPCVES
                     CPVHAISMKDINAPPVNDFEKCIGCTKCVGICPGLAIFVVRVKGKKAWVTLPYEFVPV
                     PQVGDSVNALDRTGTIRGKAIVKKVVKQGKTMVITIETDSGLAMDIRNIKV"
     gene            complement(3079..4539)
                     /locus_tag="VN377_00060"
     CDS             complement(3079..4539)
                     /locus_tag="VN377_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR26808.1"
                     /translation="MRIRSHPIIDFKKRNELPFFYQKKKVIGEEGDTIASALHAAGVR
                     TLTRSIKYDSPRGFFCGIGKCSSCLMRVNGIPNVRTCIAPLNEGITVEMQDSLPMLPD
                     AKFYGEPKKTIQVDVLVVGAGPAGLCAAIEGAKQGASVLIVDENQSLGGQLIKQTHKF
                     FGSKQERAGTRGIDIAKQLESDVRSLQNERKIQVMTDATVIGYYESDRRHRFGIVQRC
                     DYQSRLYDVRCEAAILACGAMENMLLFPGNDLPGVYGAGAVQTLMNVYGVKPGERVLM
                     VGAGNVGLIVSYQLLQAGIAVDRVVEAAPVIGGYNVHAAKLRRCGIPIQTSHSIKEVC
                     GEKKVEGAVVVRLDENWKPIKDSEEYIKCDTICLAVGLTPSTRLLSQIGVQLNFIPEA
                     GGYVALHDDRMQTSIKGIYVAGDASGIEEASTAMIEGKIAGLAAAIQLGRSKKKKLLV
                     QYHEELDQLRAGPFGEKPRIAKQKIAKLEETIHASI"
     gene            complement(4604..5029)
                     /locus_tag="VN377_00065"
     CDS             complement(4604..5029)
                     /locus_tag="VN377_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HWR26809.1"
                     /translation="MGEIVLSNETVQYINLASKYSGANILDCVVEDDRVVFIVEKGQL
                     GIAIGSKAKNLERLRMLFKKIVKFVEFDEDKTRFVVNLCKPYNVTKVSFEENDGTSVA
                     RIEVNPRDKSKLIGKGGRNINMIRKMAQRHHQIRDIQII"
     gene            complement(5035..5337)
                     /locus_tag="VN377_00070"
     CDS             complement(5035..5337)
                     /locus_tag="VN377_00070"
                     /inference="COORDINATES: protein motif:HMM:NF002172.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HWR26810.1"
                     /translation="MVDVNKALKDVSKKGTISLGEKQTKAAIQKGTAKLVVMASNCPF
                     AKTITILANDKKVPLFTYASTGVELGYTCGKNYSVSCFAVLQEGESNILQLVKQRK"
     gene            complement(5338..6768)
                     /gene="rpoA2"
                     /locus_tag="VN377_00075"
     CDS             complement(5338..6768)
                     /gene="rpoA2"
                     /locus_tag="VN377_00075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HWR26811.1"
                     /translation="MAKLISKKKTAPKKTEKKKPVAQTKKPVLKPVSKSKPAQLKKKI
                     KEVKVTAGKKKPGEATKQPVAAEKKKEPERAFLPPPTKKEEEPKEYKTIRAEIHKLLK
                     NQYLPISIIDGIIERSARDKKIEGKLKNIIEKALNEYAKNRIDPTEACGMISAQSIGE
                     PGTQMTMRTFHYAGVAEINVTLGLPRLIEIVDARSTPSTPMMNIYLRDEYRVNPDLAK
                     EIANKIEITRLTDVADIEMDLVNIYISIKPNKKTMEKKGITIEDLLERVQSVRKLSAK
                     IEKENIRVTLDEPGYKVLQNTNEALKNLKIKGIDGIKRVIIRKEPNEGYVIYSEGSNL
                     KEVLDIEGVDPYRTTTNDIHAIARELGIEAARNMIIQEAYSTLSEQGLNVDLRHIMLV
                     ADVMTADGTVRAIGRHGVSGEKSSVLSRAAFEITVNHLLLASQRGESDELNGVAENII
                     VGQPVNLGTGAIELVMNTKKPKQKGK"
     gene            complement(6773..9439)
                     /locus_tag="VN377_00080"
     CDS             complement(6773..9439)
                     /locus_tag="VN377_00080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HWR26812.1"
                     /translation="MAGRNITKKIGALEFSVLSPNEIRKMSATKVITADTYDDDGFPI
                     TMGLMDQRLSVIEPGLRCKTCGLKVGKDKCPGHFGHIDLAMPVIHVGFVKTIRNSLRA
                     TCRKCGRILLTDKELVEHRKTIIKNNTEGRDNAYVFKMIISSAVKTEGCPRCGTQSGK
                     IDLDKPTSFRENGKKLTPTEIREWLEKIPDEDLPLLDINVKSARPEWMVLTVLPVPPV
                     TVRPSVTLESGERSEDDLTHKLVDVIRINQRLQENRDAGAPQLIVEDLWELLQYHVTT
                     YLDNQTSGIPPARHRSGRPLKTLAQRLKGKEGRFRSNLSGKRVNFSARTVISPDPNLS
                     INEIGVPMEIARELTLPVRITIQNMEWCKNLIKQTALAEKPGENYRPRVNYVKRFREG
                     VEQRMKVTEKNAEDIAEKLELGFIIERQLMDGDIALFNRQPSLHRMSMMAHRIKVMPN
                     RSFRFNLSVCPPYNADFDGDEMNLHLLQGEEAKAEAEILMKVQENLMSPRFGGVIIGG
                     LHDHISGCFLLTYKDKMFSKEKTSEILATFKYKGKFPDVHEMNGEHLVYGRDIFSVLL
                     PKDLSLEYKAKACFHCDECKAADCPNGAYVIVKNGKLKQGIVDENSIGSFKGRIVERI
                     IRDHGTAAGRIFIDDVTRVGIAVVTIIGFTTSIDDEDIPAEAKRQIGEGLQKAQNKIN
                     TLVTAFEHGELESLPGRSLEETLEMEIMRVTGRARDMAGEIAGKYLGLNNSAVIMAKS
                     GARGSMLNLSQMSGCVGQQAVRGERISRGYKYRTLPHFKKGDLGASAKGFVANSYKSG
                     LNPTEYFFHSMGGREGLVDTAVRTSRSGYMQRRLVNALEDLKVESDGTVRHTGGEIIQ
                     FMYGDDGVDPTRSTVGKAVDLDRIIYDVKLEG"
     gene            complement(9452..>10226)
                     /locus_tag="VN377_00085"
     CDS             complement(9452..>10226)
                     /locus_tag="VN377_00085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178286.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HWR26813.1"
                     /translation="VKIKVREQRIPELGDKFASKHGQKGVIGYVVSAENMPFTEDGMS
                     PDLIINPHAIPSRMTVAHVLEMIGGKVGSLEGRIVDGTAFSGETEDQLRTALVNNGFN
                     HAGKEIFYDGLTGRPLECDVFVGCIYYQKLHHMVAGKIHARSRGPVQILTRQPTEGRA
                     REGGLRFGEMERDCLIGHGASMVIKDRLLDESDITMKYVCNTCGHIAMIDRHGRLHCS
                     ICGDKADIHPVEMSYAFKLLIDELKSLVVVPRIKLESLV"
BASE COUNT         2457 a         2485 c         2077 g         3207 t
ORIGIN      
        1 gtttttatac tctaacctgg agtgagtgat tctgaaggtt agtattgtta ttcctgctgt
       61 caatgaggaa cgaggtatcg gtaaaaccat tgatgcagtc aacaaagaat atttcaacaa
      121 acaacactgg gatcttgaga ttctcctagt cgatgggaat tccaaggata aaacaaggga
      181 gatcgcaacc agtaagggag taatagttct actcgagaaa caaaaaggat acgggagagc
      241 ttataaagtc gggattgcac acgcgactgg agatattatc gtcactggtg atgcagatgc
      301 aacctatcca tttcatctta ttcatgacta tatccaaatg cttctagacc gacaccttga
      361 ttttctgact acggatcggt tcgcagcact caaacatggt tcgatgtctc tcaagcaccg
      421 tttcgggaac ctcaccttgg cctggacact ccgcatcctt ttcctagtca atatcaggga
      481 ttctcaatca ggtatgtgga tcttcaaacg ggatgccctt accaagattc agcctttgaa
      541 tgacttcaac gatggaatgc cgttttcaga agaaataaaa atagagatgt ttacgaacaa
      601 acacatcaaa gcaaaagaga tcccctcgac cctttttgcc agagaaggtc aagtgaagct
      661 cgaaagcttc aaggacggga taaagaacct gcggttcctt tttaaaaaac gagttacccc
      721 ttcaaaaaga cactgagatg caaaaggtat tcaacgtgct tttccatcct ctttctttga
      781 ggaagacgta tggatccgtt gatgactatc tgggtatttt tcattggatg ttttttcgga
      841 gttatccttg gggtgatgct ctcctatcga actgcagtaa caccgttgca acacacgata
      901 tcaaaactca cttctcaaga tcaacagtat catgagacga tgaaatacta cccctatgat
      961 cgtgaaaagt tccgattcat tggtggacca atcgatggga ttcaatttga agaagactgt
     1021 atcatatttg tcagattgaa ggaaggaaac attccgcggt caaaagaaca agaaaacata
     1081 aagaatcttc tggagaatgg gaagatccag tggtttgagt ttccgacgaa ataaagctca
     1141 caatctgtaa atgaaggatt tttttttttt tatcaaatcg gcacgatacc aaagaacggt
     1201 ttattctctt tccattttag cctacaacgg agagttcttt ttcgatgttc tttcctttga
     1261 atcggtccag agtgagattc tcaagtacct ctgatttttt tccttcagtg atcagcgtgg
     1321 taagcaaccg agaaaccatg ggggatatca taaacccatg accgctgaat ccattacatt
     1381 ggaggaaacc tttcatttct tttacctcac caaggattgg tcgtgcatca ggggtcacat
     1441 cataaaaccc tgtccaatgt cgtaacagat tgacatgttt caataccggt gcatacctgg
     1501 tcaatgtccg cgacatatgt tcaaggaacg aaaacgtcgg catcgttttg tatcctcctt
     1561 tctcctcagg gattggtatg ccaccaacga tctgtccttc tttctgttga ctaaaataga
     1621 tcccgtcttt aaaggaaata accatcgctt cgaacaatgg ttgtattcgt tctgttgcca
     1681 tgatctcttt acggaatgga gcgagcggaa gtgtaatgtt caccatctct gcgatagttt
     1741 tcgaatcaat ccctgctgca ttcaccacta catcggtttg aattgtcccc ttatcagtta
     1801 ctacagcatg tatagccttt tttgatgttt tgaggttcac aaccgtggta tgtgtgttga
     1861 tttcagcacc aaggttccgt gctgcgtcag catacgcaaa ggtcgtcctg aatggatttg
     1921 catggccatc cgttgggcag aacgttgccc ctatcgcttt catccctttg acatcaagga
     1981 tagggacgat atcggtgatt tcctcaggtt ttaagatatc gacttttaat ccaagggatc
     2041 gttgcattgc gacattcttt tctgcttgtt tcaattcttt tttatcatga atggcgatga
     2101 gatatccgcc ttgacgaaac tcaatatcct ggcctagctc acgacttaat tgttcaaaaa
     2161 tcttgacgct ttggatagca agtgatgcat tttcacgggt cgaccattgc tgtctgatcc
     2221 ctgcgccaca tgcaccagtc gcaccagagg agagatattt cttctcaacg acgacgacat
     2281 cgaggtcttt ttttgccaga ttatatgcaa gggaacaacc atttacgccg ccgcctatga
     2341 tgacgacatc tgctttactt ttcatctttc tcacttgcca gtgttccaag ggagacaggg
     2401 attaatggag gtctcactgt cggaagtggt atatttgcta ccttcttccc agtttttctt
     2461 tcaaggatac gagcaaccag ttggatacag actcgtcctt gacatggccc cattcctatc
     2521 cgtagtttct tgcgaagttc ctcaatatcg gtgtatcctt tgtcaattgc ttgaatcacg
     2581 tcttcttcgc tgatgtcttc acagcgacac acgatacttc gcttcatggg ttagaccttt
     2641 atgttcctga tatccatggc cagaccactg tctgtttcta ttgtgatgac catggttttt
     2701 ccttgtttga ctactttttt tactatcgct ttgccgcgaa tagtaccagt cctatccagc
     2761 gcgtttacac tatctccaac ttgaggaaca ggtacaaatt catacgggag cgtcacccat
     2821 gctttctttc cttttactct tacgacaaat atggctaagc ctgggcaaat accgacgcat
     2881 tttgtgcatc cgatgcattt ctcaaaatca ttcactggtg gtgcattgat atctttcatg
     2941 gagatcgcat ggactggaca tgattctacg caggggttac atggaatctc ctgcacacat
     3001 tcaaggatag cgaccccttt ctctagttgt ttttcggagg ggatttgtaa atctttagca
     3061 gaaaggattc cggttttttc atattgacgc atggattgtc tcctctagtt tggctatttt
     3121 ttgttttgcg attcgtggtt tttctccgaa tggacctgcg cggagttgat caagttcctc
     3181 atgatattgt accaggagtt ttttcttctt cgatcgtccg agctggatgg cggcggcaag
     3241 acctgcgatt ttcccttcga tcatggctgt cgatgcttcc tcgattcctg aggcatcgcc
     3301 tgccacgtaa atccctttta ttgaggtttg catcctatca tcatggagtg cgacgtatcc
     3361 accagcctcg gggataaagt taagttgaac gccaatctgg gatagcagac gggttgacgg
     3421 ggtcaatccg accgcaagac aaatcgtatc acatttgata tattcctccg agtcttttat
     3481 tggtttccaa ttctcgtcaa gtcgtacgac aacggcacct tccactttct tctcaccaca
     3541 cacttcttta atcgaatggg acgtctgtat tggtatacca catcgtcgta gtttagctgc
     3601 atggacatta tatcccccta tcactggtgc agcttccacg acccgatcaa ccgcaattcc
     3661 tgcctgcaac aactgatacg agacgattaa accgacgttc ccagcaccca ccatgagtac
     3721 tcgctcacca ggttttaccc catagacgtt catgagagtc tgaacagccc ccgcaccata
     3781 cacaccagga aggtcatttc cagggaacaa cagcatgttt tccatagcac cacaagcaag
     3841 aattgctgcc tcacatcgaa catcatacaa cctactttga tagtcgcagc gctgaacgat
     3901 accaaaacga tgccttctgt cactttcata ataaccgatg accgtcgcat cggtcatcac
     3961 ctggattttt ctttcatttt gaaggctccg tacatcagat tccaattgtt ttgcgatgtc
     4021 aataccacgg gttcctgccc gttcttgttt cgaaccaaaa aatttatgag tctgcttaat
     4081 caattggcct ccaagagatt ggttttcatc tacgattaaa accgatgcac cttgttttgc
     4141 gccttcgata gctgcacaga gaccagcagg accagcgcca accaccaaga catcgacctg
     4201 aattgttttc ttaggttctc cgtagaactt tgcgtccggt aacatcggga gagaatcctg
     4261 catctcaacg gtaatcccct catttaacgg tgcgatacag gttcgtacat ttgggatacc
     4321 gttcacccgc atcagacaag aggagcattt cccgatgcca caaaaaaaac cacgtgggct
     4381 gtcatattta atactccttg ttaatgttcg aacccctgcg gcatggagcg cagaagcaat
     4441 ggtgtcccct tcctcaccaa tgaccttctt tttctgatag aaaaatggaa gctcattacg
     4501 ttttttaaaa tcgataatcg gatgagaccg tatacgcatg ctgtgccccc tatcatgcct
     4561 ggtatatact accttttata agattttgcc taggagaaag ttattaaatt atttggatgt
     4621 ccctgatttg atgatgacgt tgggccattt ttctaatcat gtttatgttt cgtccaccct
     4681 tgcctatgag ttttgacttg tcccttggat tcacctctat tcgtgcaaca cttgtcccat
     4741 cgttttcttc aaagctcacc ttcgtcacat tatacggttt gcagagattc acgacaaacc
     4801 tggttttatc ctcatcgaat tcaacaaact tcacgatttt cttaaaaagc atccgcagcc
     4861 tctcaagatt tttagctttg ctccctatcg caataccaag ttgccctttc tcgacaataa
     4921 atacaacccg gtcatcctcg acaacacagt caagaatgtt tgctcctgaa tacttggatg
     4981 caaggtttat gtattgtacg gtttcgtttg atagaactat ctctcccatt ctgcttattt
     5041 cctttgtttc acaagctgca ggatatttga ttccccttct tgaagcactg caaaacaaga
     5101 tactgaatag ttcttcccgc aggtataccc aagttcgaca ccggtggaag cataagtaaa
     5161 caatggtact tttttgtcgt tggccagaat cgtaatggtt tttgcgaacg ggcagttact
     5221 tgccatgacc accagttttg ctgtaccctt ttgtatcgct gctttcgtct gtttttctcc
     5281 aagactaatt gtgcctttct ttgatacatc ttttaatgct ttatttacgt ctaccattta
     5341 ctttcccttc tgttttggtt tttttgtatt catcacaagt tctattgcac ctgttccaag
     5401 attcacgggt tgtccgacga tgatgttctc agctacacca ttcaactcgt ctgattctcc
     5461 tcgttggctg gcgagcaaga gatgattgac ggtaatctca aatgctgctc gtgaaagaac
     5521 ggaacttttt tcaccactaa ccccatggcg acctatagcc cgaacagttc catccgcggt
     5581 catgacatca gcaacgagca tgatatgtct cagatcgacg tttaaccctt gttctgacag
     5641 agtggaatat gcttcttgga tgatcatgtt tcgtgccgct tcaataccga gttctcgggc
     5701 aatcgcatgg atatcgtttg tggtcgtccg gtacgggtca acaccctcga tatctaacac
     5761 ttctttcaga ttgctccctt cgctgtaaat cacatagcct tcatttggtt cttttcggat
     5821 gatgacccgt ttaatgccat ctatcccctt gatcttgagg ttctttaatg cctcgttcgt
     5881 gttctggaga accttatacc ctggctcatc cagggtaact cttatgttct ctttttcgat
     5941 ttttgcggat aactttcgaa cggattgtac gcgttctagc aaatcctcaa tggtgattcc
     6001 ttttttctcc attgttttct tatttggttt aatggaaata tagatattta ctaagtccat
     6061 ttcgatatca gcaacatcgg taagtctagt gatctcaatt ttgttggcaa tctcctttgc
     6121 taaatctgga tttacccgat attcatctct aaggtagatg ttcatcatcg gtgtggaggg
     6181 tgtggacctc gcatccacga tttcgattaa ccgcggtaga ccaagggtga cgttgatttc
     6241 cgcgacacca gcgtagtgga acgttctcat cgtcatctgg gtccctggtt ccccgatact
     6301 ctgggcactg atcatgccgc atgcttccgt cggatcgatt cgattttttg catattcatt
     6361 caatgctttc tcgatgatgt ttttcaattt tccctctatt tttttatctc gtgcggaacg
     6421 ttcaataata ccatcgatga tagaaatagg gaggtactga ttttttaata gtttgtgtat
     6481 ttctgctcgt atcgtcttgt attcttttgg ttcttcttct tttttagttg gtggcggaag
     6541 gaatgctcgt tccggctctt ttttcttctc tgcagcaaca ggttgcttcg ttgcttcgcc
     6601 tggttttttc ttccctgcag tgaccttcac ttcctttatt ttcttcttca actgtgcagg
     6661 tttacttttc gatacaggtt tgaggactgg cttcttggtt tgtgcgacag gtttcttctt
     6721 ctcagttttt tttggagcgg ttttcttttt agaaatgagt ttagccatgt gattagccct
     6781 ccaatttcac atcatagatg atacgatcaa gatccactgc tttacctacc gtactgcgtg
     6841 tcgggtcgac cccgtcatca ccatacatga attggatgat ctccccgccg gtatgacgca
     6901 ctgtcccatc gctttccact ttgaggtctt cgagagcatt gaccaaccga cgctgcatat
     6961 acccggatct tgaggttcgt actgcggtat caacaagacc ctctcttcct cccatcgaat
     7021 ggaagaaata ctctgtcggg ttcaaaccgc ttttgtaact gttggcaaca aagccctttg
     7081 cgctagctcc aagatcacct tttttgaagt gaggtaacgt cctgtactta tatcctctcg
     7141 atatccgttc tcctctgact gcctgctgtc caacacaccc tgacatctgc gagaggttca
     7201 gcatagaacc tctggcacct gattttgcca tgatgaccgc gctgttgttc aacccgagat
     7261 atttacctgc gatttcacca gccatgtctc ttgctcggcc agtgacgcgc atgatctcca
     7321 tttctaaggt ttcttccagg cttcgaccag gtagggattc aagctcccca tgttcaaatg
     7381 cagtgaccaa ggtgttgatc ttgttttgtg ctttctgcaa tccttctcca atctgacgtt
     7441 ttgcttcagc cggaatatcc tcatcatcga tactggtggt aaacccaatg atggttacca
     7501 cggcaatccc aactcgtgtg acgtcatcga tgaagatcct gcctgctgcg gtcccatgat
     7561 ctctgatgat acgctctacg attcttcctt tgaatgaccc gatgctgttt tcgtcgacaa
     7621 tcccttgttt tagtttaccg tttttcacaa tcacataagc gccattggga caatctgcag
     7681 ctttacattc atcacaatga aaacatgctt ttgccttata ttcaagacta agatcttttg
     7741 ggagtaatac actgaagata tcccttccgt acacaagatg ttcaccattc atctcgtgga
     7801 catcagggaa tttccctttg tacttgaatg tcgcaagtat ctccgatgtt ttctctttcg
     7861 aaaacatttt atccttgtag gtaagcagga aacaacctga aatatgatca tgtaacccac
     7921 caatgatgac cccgccaaaa cgaggagaca taagattctc ctgtactttc atcagtatct
     7981 ctgcctcagc ttttgcttct tcaccctgta gaagatgcag gttcatttca tcaccatcaa
     8041 aatcagcgtt atatggggga cagactgaga ggttaaaacg gaatgatcgg ttcggcatga
     8101 ctttgattcg atgtgccatc atcgacatcc gatgtaacga cggctgacgg ttaaataatg
     8161 cgatatctcc atccatgagc tgccgttcta tgataaaacc aagctcaagt ttttctgcga
     8221 tgtcctcagc gttcttctct gttactttca ttcgttgttc gacaccctca cggaatcgtt
     8281 tcacataatt cacccggggc cgataattct caccaggttt ctcggctaac gcagtttgtt
     8341 ttatgaggtt tttacaccat tccatgttct ggattgtaat acgaacaggt aacgtgagct
     8401 cccgtgcgat ctccatgggg acaccgatct cattgatact aagatttgga tccggtgaaa
     8461 tgacggttct tgcagagaaa ttcactcgtt tccctgataa gttactgcgg aatctaccct
     8521 cttttccttt caatcgttgc gcgagtgttt tcaaaggtct tcctgatcgg tgtcgtgctg
     8581 gtggaatccc tgaggtttgg ttatccagat acgtggtgac atgatattgc aggagctccc
     8641 agaggtcttc gacgattaac tgaggcgccc cagcatcacg gttttcctgt aatcgttgat
     8701 tgattctgat gacatcaacg agtttatggg tcagatcatc ttccgaccgt tcccctgatt
     8761 ccagggtcac cgacggtcgt acggtgactg gcggtactgg taagacggtc aggaccatcc
     8821 attctggtcg tgccgacttc acattgatat ctagcaaagg aagatcctcg tcagggattt
     8881 tctcaagcca ttcacgaatc tcagttggtg tgagtttctt accgttttct cggaacgagg
     8941 ttggcttatc caaatcgatt ttaccgctct gtgttccgca tcgtggacat ccttccgttt
     9001 tgaccgcact gctgataatc atcttgaaca cataggcgtt gtcgcgtccc tcagtgttgt
     9061 tcttaataat ggttttccga tgctctacca gttctttatc cgtgaggagg attcgaccgc
     9121 atttccgaca ggtcgcccgg aggctattcc gaatcgtctt gacaaatcca acgtggataa
     9181 ccggcatagc aagatcgata tgtccaaaat gccctgggca tttgtccttt ccgactttca
     9241 acccacaggt cttacatcgt aatccaggtt ctatgacact taagcgttga tccatcaacc
     9301 ccatggtgat aggaaaccca tcatcatcgt aggtatccgc agtgatgacc tttgtcgcac
     9361 tcatcttgcg gatctcattg ggtgataata ctgaaaattc aagagccccg attttttttg
     9421 tgatatttcg tccagccatt ctttttacct cctacaccag cgactccagt ttaatccgtg
     9481 gtaccaccac aagagatttc aactcgtcaa tcaaaagttt gaacgcatag ctcatttcaa
     9541 ctgggtgaat atctgcttta tccccacaga tggagcaatg gagtcgacca tgacggtcga
     9601 tcatcgcgat atgaccgcac gtgttacaaa catacttcat cgtgatatct gattcatcga
     9661 gtaatcgatc cttgatgacc atcgaggcac catgaccaat aagacagtca cgttccattt
     9721 ctccaaatcg gagaccacct tctctggctc gtccctctgt tggttgtctg gtgaggattt
     9781 gtactggtcc tcgtgatcta gcatggatct tgcctgcgac catatgatgc agtttctggt
     9841 aatagataca tccgacaaac acatcgcatt ccaatggtcg tccggtgagt ccgtcataaa
     9901 agatttcttt tccagcatgg ttgaatccat tgttcacgag cgctgttcgt agttgatcct
     9961 ctgtttcccc actaaaagcg gttccatcca cgattcgtcc ttcaagtgat ccgacttttc
    10021 cgccgatcat ctcaaggaca tgcgcgaccg tcattcttga agggattgca tgagggttaa
    10081 tgatcaaatc aggagacatc ccgtcttcgg tgaatggcat gttttctgca gatacgacat
    10141 atccaatgac acccttctga ccatgttttg atgcgaattt atcccctagc tcagggattc
    10201 gttgttctct aacttttatc ttgacc
//
LOCUS       DATMXJ010000003         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_26179, whole genome shotgun sequence.
ACCESSION   DATMXJ010000003 DATMXJ010000000
VERSION     DATMXJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26179"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..381)
                     /locus_tag="VN377_00090"
     CDS             complement(61..381)
                     /locus_tag="VN377_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26814.1"
                     /translation="MISPFVILPYDTMKKKGKAVLKKAVVKSSKSKVSKKVSKTTRKV
                     MVGEDEFCPTCMEWRPYDEITGKCKVCGCLIKKIGEARKITDEYDLKDFSNEHDEPQD
                     TSEF"
     gene            516..1097
                     /locus_tag="VN377_00095"
     CDS             516..1097
                     /locus_tag="VN377_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HWR26815.1"
                     /translation="MDLLAFYNSDIFKWVILPLLIFSARLIDVSLGTLRIIFVSHGLK
                     YIAPLVGFVEINIWLLAIGQIMQNLNNLACSLAYAGGFSMGAFIGMLIEEKLSIGMVM
                     VRIICKHDASELIKSLRETEYGVTTHDAEGINGPVKIIFAVIRREDLHDILNKIHKIH
                     PHAFYSVEDVRSVGEAMFPYHRHRLFQWHRKGK"
     gene            complement(1119..1847)
                     /locus_tag="VN377_00100"
     CDS             complement(1119..1847)
                     /locus_tag="VN377_00100"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: protein motif:HMM:NF003075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HWR26816.1"
                     /translation="MEQSGQQYDEASTIFSPDGRLYQVEYAREAVKKGSTTMGFKWKD
                     GVLLLAHKPETSRLVNLRSLEKIYQIDSHIACVATGLVADGRHLVEIAREEAQWNKVR
                     YDEPISVKELVNILCEYKHMYTQFDGVRPFGVVLLIGGIDTTGKHLFSTDPSGAYLGY
                     KAVCEGKKSNDAMTQFTKNYTMDISLQQALELGFQTMKKITKPKFNAEMVEAAVIDEK
                     TGYYKLSAEALKKVVEKHNSEQKE"
     gene            complement(1907..2182)
                     /locus_tag="VN377_00105"
     CDS             complement(1907..2182)
                     /locus_tag="VN377_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HWR26817.1"
                     /translation="MVSRDDTTYVIYPLYFDRSISRLKGRKVSQKHAVEKPSLENIAK
                     AAKSLGLNPILEKNAAHSSTPWKKDGRIIIEKKGPKSSLLLQISNRL"
     gene            complement(2199..2582)
                     /locus_tag="VN377_00110"
     CDS             complement(2199..2582)
                     /locus_tag="VN377_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HWR26818.1"
                     /translation="MAVWQSNSKRSSTGRRIRYARNKRRFEIGRENHLTTIGVMKRKP
                     FRTRGNNKKSRVLTADSAYVVDPKTNKTVKTEIVTVVENSANIHYVRRNIMNKGAIIN
                     TKIGKAKITSRPGQSGVINAVLLSK"
     gene            complement(2817..>3774)
                     /locus_tag="VN377_00115"
     CDS             complement(2817..>3774)
                     /locus_tag="VN377_00115"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR26819.1"
                     /translation="HWSGDASGSNPVTTVTMTGDRSVVAHFSQNVYTLSVSVDPVAGG
                     SVGAVPSAPYYYGTVVTLTAGANPGYSFDHWSGDASGSNPVTTVTMTGDRSVVAHFTQ
                     EGYTLTITIDGQGTVIKDPEQTTYAYNSIVKLTAIADENWVFNSWGGDLSGNQNPQNI
                     TMNANKTVTAHFTYTGDDMIPPVVKIVKPGNNGIYIFNKLVIAFGMIPMPIIVQMITI
                     EVNASDNESGIDHVEFFVDGVSKSIDDSEPYSYDWRDLISGKHTITVKAYDKAGNNAT
                     SPDLLVFKWRFHPMLVVLLLLLGLVWQNEKPTTAAATNQTSG"
BASE COUNT          987 a          828 c          785 g         1174 t
ORIGIN      
        1 ttagaatgct taacaaatta tccctatata tttacaacat ttacaaaata agtattacta
       61 ctaaaactca ctggtgtcct gtggttcatc atgctcgttt gaaaaatctt tcagatcata
      121 ctcatcggta atcttccttg cctcaccaat ctttttaatg aggcacccac agaccttaca
      181 tttaccagtt atctcatcgt acggtcgcca ttccatacac gtcggacaaa actcatcctc
      241 accgaccatc actttccgag tcgtcttact gactttcttc gacacttttg attttgaaga
      301 tttaaccact gcttttttta ggacagcctt tcccttcttt ttcatagtat cgtacggaag
      361 aatgacaaaa ggagatatca aaaaagattt atattgactt ttctacccat gcgcttttcc
      421 cctgctttat aaataagggg caaaaaaaat aaagatttat agaggggaaa acaatacctg
      481 cagatagcct cacaaggcaa cgcaggggaa aaaacatgga cctactcgca ttttacaatt
      541 cagatatctt caaatgggtc attcttccac ttttgatttt ctcagcacgg ttaattgatg
      601 tcagccttgg gaccctccgg atcatctttg tctcccatgg cctgaaatac atcgcacctt
      661 tggttggttt cgtagagatc aacatctggc tgcttgccat cgggcaaatc atgcagaacc
      721 tcaataatct cgcctgttcc ctcgcgtacg caggtgggtt ttctatgggt gcttttatcg
      781 gcatgcttat tgaggaaaaa ttatcaatag gtatggtcat ggtacggatc atctgcaaac
      841 atgacgcttc agaactcatt aaatccctca gagaaacaga atacggagtc accactcatg
      901 atgcagaagg catcaatggt ccggtaaaga ttatcttcgc agtcatccgt cgagaggacc
      961 ttcatgacat tctcaataag atccataaga ttcatccgca tgcgttttat tcagtggagg
     1021 atgtccgatc tgtgggagaa gccatgtttc cgtaccatcg gcatcgactg ttccaatggc
     1081 atcgaaaagg aaaataaaca aaaaaatcgg atgacgatct attctttttg ctcggagtta
     1141 tgtttttcta ctaccttctt gagagcctcg gctgagagct tatagtaccc ggtcttttca
     1201 tcgatgaccg ctgcttcgac catttctgca ttgaatttcg gtttggttat ctttttcatt
     1261 gtttgaaaac caagttcaag tgcttgttgt agtgagatat ccatcgtata gtttttcgta
     1321 aactgggtca tcgcatcgtt gctttttttg ccttcgcaaa ccgccttgta ccctagatag
     1381 gcgcctgaag gatctgtcga gaagaggtgt ttacctgtgg tatcgatacc accaatcagg
     1441 agcacgacgc caaaaggtcg gactccatca aactgagtat acatgtgttt atattcgcag
     1501 aggatgttga caagttcttt cacagagata ggctcatcat agcggacttt gttccactgt
     1561 gcttcttccc gtgcgatctc aacaagatgc cgcccatcgg caaccaggcc agtcgcaaca
     1621 caggcgatat gtgagtcaat ttgatagatc ttctcaagag atcgtaaatt gacgagtctg
     1681 ctggtctctg gtttatgcgc tagcagtagc accccatctt tccatttgaa gcccatggtt
     1741 gtggagcctt tcttgactgc ttctcgtgca tattccactt gatataatct tccatcagga
     1801 gaaaaaatcg tgcttgcttc atcgtattgt tgtcctgatt gttccataac ccatctccaa
     1861 gagagtttta tcagaatgct atgttgagat tatatacttt cttgccttac agtcggtttg
     1921 atatctgcaa gagaaggctg ctttttggtc cttttttctc aataatgata cgtccgtcct
     1981 ttttccaggg tgttgatgaa tgtgccgcat ttttttctaa aatagggttt aacccaagtg
     2041 attttgctgc ttttgctatg ttctccaggg aaggcttctc taccgcatgt ttctgtgaga
     2101 ccttccgtcc tttcaagcga gaaatggacc ggtcaaaata cagtgggtat atcacgtagg
     2161 tagtatcatc tctagagacc attttgatca cgacagtctt atttcgagag aagtactgcg
     2221 ttaatcacgc cgctttgtcc aggtcgagag gtgattttgg ctttaccgat tttcgtgttg
     2281 ataatggcac ctttgttcat gatgtttcgt cggacataat ggatgtttgc tgagttttcg
     2341 acgacagtaa caatctcagt tttaacggtt ttgttggttt tcgggtcgac aacataagcg
     2401 ctatctgcgg tgaggacccg agatttttta ttattgcctc tggttctgaa tggtttacgt
     2461 ttcatgaccc cgatggtagt caggtgattt tccctgccta tttcgaaccg tctcttattc
     2521 cgtgcatacc gaatacgtcg tccagtggaa cttctttttg agttactttg ccaaactgcc
     2581 atgaggagtc cgaatgaaga acgaatattt aaaggatatg ggcgtttagg aggaaaactg
     2641 ttaaaaatga atgaatcggg gggttttaaa tttctgcgta tttaacctcg caacggttct
     2701 tccatagtag attatttatt ttttatgaag cgatctgatt atatgggtca attgtcgatg
     2761 tgtaatctaa taaaaagaaa aaaaagagtt tttagagtat ggttcttatc cgaaatctaa
     2821 ccgcttgttt gattggtagc ggctgcggta gttggttttt cattttgcca tactaaaccg
     2881 agaaggagga ggaggacaac gagcattgga tggaatctcc atttaaacac gaggaggtca
     2941 gggcttgtcg cattgtttcc cgctttgtca taggctttca ctgttattgt atgctttcca
     3001 gaaataaggt ctctccagtc gtatgaatat ggctctgaat catcgatgct ttttgatact
     3061 ccgtcgacga agaactctac atggtctatt cctgattcgt tgtcagacgc attgacttcg
     3121 attgttatca tttggacgat tatcggcatc ggaatcatac caaaggcgat gacaagcttg
     3181 ttaaagatgt aaataccgtt gttgcctggt tttactattt tgacgacagg aggtatcata
     3241 tcatccccgg tatatgtgaa atgggcagtc actgttttgt tcgcgttcat cgtgatattt
     3301 tgaggatttt ggttacctga gagatctccg ccccatgagt tgaaaaccca gttttcgtct
     3361 gcaatggcag taagcttaac gatgctattg taggcatacg tggtttgttc tgggtcttta
     3421 atgactgtcc cttgaccatc gatggtaatt gtgagtgtgt atccttcttg tgtgaaatga
     3481 gcaacaaccg acctatcacc agtcatcgta acagtagtca ccggattcga cccagaagca
     3541 tcaccactcc aatgatcaaa actataccca ggattcgcac cagcagtcaa cgtcaccaca
     3601 gtcccataat aatacggagc agacggaacc gcaccaacag acccaccagc aacaggatca
     3661 acactcacag acaacgtata cacattctgc gaaaaatgag caacaaccga cctatcacca
     3721 gtcatcgtca cagtagtcac cggattcgac ccagaagcat caccactcca atga
//
LOCUS       DATMXJ010000004         5423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_5263, whole genome shotgun sequence.
ACCESSION   DATMXJ010000004 DATMXJ010000000
VERSION     DATMXJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5263"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1482
                     /locus_tag="VN377_00120"
     CDS             25..1482
                     /locus_tag="VN377_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HWR26820.1"
                     /translation="MKTITIDPITRLEGHGKITIFLNEQGDVENAYLQIPELRGFEKF
                     SEGRRAEDMPQITSRICGVCPVAHHFAATKALDAAFHVDPPVTAKKLRELLYCGYLIY
                     DHILHFYFLGGPDFVVGPEAPAAKRNILGVIEKTGIDVAKDVIKHRAYGQRITAILGG
                     KATHPVCGLPGGVSKGLSKDEVKEIKDMVASCNTFAKFTLNLFDDIVLKNKAYVDLIC
                     SDPYTLKTYNMGLVDKHNKVNFYDGMIRVTDQEGDQLEKFDGKDYTKHIAERVEEWSY
                     MKYPYLKTIGWQGLSDGKKSGIYRVGPLGRLNASDGIATPQAEKEYKRMFDTLGGKPV
                     NSTLAFHWARLIELLYATERAKELIEDADITSTHIRNPVGTPGEGIGVVEAARGTLIH
                     HYHVDKNGLIEKANMIVATTNNAGAITMSVRDAAKGVIHKGQVSDGLLNKVEMAFRAY
                     DPCFGCATHTLPGGLPMTVNIYDHEKKLYKTFSQP"
     gene            1492..1950
                     /locus_tag="VN377_00125"
     CDS             1492..1950
                     /locus_tag="VN377_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HWR26821.1"
                     /translation="MKTIVLGIGNPILQDDGVGLHVIDTLRQQMNNPLVTLETASTGG
                     MNLLDMIRGYEKVILIDAVKQKESVPGEVKRFLLSDFHTVHSCNPHDVSLSEALHLAK
                     QLGEKHLPSKIILIGITVNNTYEFGEHLSSEVASAVPTAVNLVLEELKNT"
     gene            1962..2384
                     /locus_tag="VN377_00130"
     CDS             1962..2384
                     /locus_tag="VN377_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HWR26822.1"
                     /translation="MSKEFEPNILGFLCNWCSYAGADLAGTSRMKYPSNLKSIRVMCS
                     GRVDPAFVLEALRKGVDGILIAGCHPGDCHYQSGNYKTNRRIKLLKKLLEELGIDPQR
                     VRFEYVSASEGQKFATIVTEFVAEIKKLGPNPITVEKN"
     gene            2410..2571
                     /locus_tag="VN377_00135"
     CDS             2410..2571
                     /locus_tag="VN377_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26823.1"
                     /translation="MDEKKKGSVEEAAEKTGEVIGKGVKKGWGAVKSFGKGVKEGVSK
                     EKKEEEEKK"
     gene            complement(2597..3727)
                     /gene="asnA"
                     /locus_tag="VN377_00140"
     CDS             complement(2597..3727)
                     /gene="asnA"
                     /locus_tag="VN377_00140"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HWR26824.1"
                     /translation="MTANKKADLAGPGISTYKEVEKVLPKNYKSLLTPKETQKAIFSA
                     KNYIEEHLGTELNLMMVTVPLIVDKESGVNDYLDRDGSRTPVEFPCGLGIQPRMQAQV
                     VQAATKWKRMALKEFDMKVGEGLNTDMRAVRKDYFLDHDHSSYVDQWDWEQVITKDQR
                     TISYLKETVNKIWKVFKGAETLILDDFPQLESKTIPNLPNKLEFFHAEEILNMYPELP
                     RKQRETAILQDHPAIFIIGIGSVLEDGYPHEMRAADYDDWITDTSKETGKQTHGLNGD
                     ILVWNPVTQRRHELSSMGIRVTPETLKKQLEFSDQTDFLNLPYHQAILNNKIPLSIGG
                     GIGQSRTYMYLLKKAHLGEVSVTVWPKELKEICAKKNIHVLE"
     gene            complement(3853..4281)
                     /locus_tag="VN377_00145"
     CDS             complement(3853..4281)
                     /locus_tag="VN377_00145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWR26825.1"
                     /translation="MKNVKEGDTVKLLCEAKLESGELCYTNDAQNPLELVVGDGKFFP
                     HVEEALHEMTEGETKVITLEAEDAFGPHIDDLVIKVPKSVFKTEVTLEVGSRIKIDVP
                     TGKSFIGTIQAMDDQTLTLDMNHLLAGKRLVVKVTILAIG"
     gene            complement(4419..>5423)
                     /locus_tag="VN377_00150"
     CDS             complement(4419..>5423)
                     /locus_tag="VN377_00150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405359.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HWR26826.1"
                     /translation="EINRKHPLRDMGIIETTNPCGEVPLLAYESCNLGSINLARMITN
                     GKLNWKKLRQTTRLAVHFLDNVIDANNYPLKETENITKANRKIGLGVMGFADLLFMLK
                     IPYESKDALQLGSRLIKFIAKEATEKSVELGKERGSFKNFTKSTWQKKYPAMRNATCI
                     TIAPTGTISIIAGCSSGIEPVFALAFIRRVMEGKQLPEINGIFKRELIRRNLYSEELV
                     RQISKTGNIKRLKLPDDLKKIFITALDITSEFHVQMQAAFQRYVDNAVSKTVNLPKNS
                     TQKDVRNIYLLAWKLKCKGITVYRYGSKPEQVLYLGTGKPIVVTTDYAGDCPTGLCPH
                     "
BASE COUNT         1450 a         1179 c         1295 g         1499 t
ORIGIN      
        1 attactaaac cggaaggtga tgaaatgaaa accattacta ttgacccgat cacccggtta
       61 gaaggccatg gaaaaatcac catcttcctt aatgaacagg gggatgtgga gaacgcatac
      121 ctgcagatcc ctgaactcag agggtttgaa aaattctcag aaggaaggcg tgctgaagat
      181 atgcctcaga tcacgtcccg tatctgtggt gtctgtcctg ttgctcatca ttttgcagcc
      241 acaaaagcgc tagatgcagc gttccatgtc gatccaccgg ttacggcaaa gaaacttcgg
      301 gaactgttgt attgcgggta tctcatctat gatcatatcc tccatttcta tttcctgggc
      361 ggtcctgatt tcgtggtcgg tccagaagcc cctgctgcaa aaagaaacat ccttggtgtc
      421 attgaaaaaa caggaattga cgtcgcaaaa gacgtcataa aacaccgggc gtatggtcag
      481 agaatcaccg cgattctcgg gggaaaagcg acccatccgg tctgcgggct ccctggtgga
      541 gtctccaagg ggctttccaa agatgaagtc aaggaaatca aagacatggt cgcatcatgt
      601 aacacctttg cgaaattcac cttgaacctg tttgatgata tcgtattgaa gaacaaagcg
      661 tatgtcgatt tgatttgttc ggatccgtac acgttaaaga cgtacaacat gggtctggtc
      721 gataaacaca acaaagtgaa tttttacgat gggatgatca gagtcacgga tcaagagggg
      781 gaccagctgg agaaatttga tggcaaagac tataccaaac atatcgctga acgtgttgaa
      841 gaatggagtt acatgaaata cccgtacctc aaaaccatcg gatggcaggg tctttcagat
      901 gggaagaaaa gcgggatcta tcgcgtcggt cctcttggaa ggctgaatgc atcagacgga
      961 attgcaaccc cgcaagcaga aaaagaatac aaacgcatgt tcgacacgtt agggggaaaa
     1021 cccgtgaact cgacactcgc gttccattgg gcacgactca tcgagctgct gtacgcgacc
     1081 gagcgggcaa aagaactcat tgaggatgct gacatcacct cgacccatat tcgaaacccc
     1141 gtgggaacac caggggaagg gatcggggtc gttgaagcag cacgaggcac cctgattcat
     1201 cattatcatg ttgataaaaa tggattgatt gagaaggcaa acatgattgt tgcgaccacg
     1261 aacaacgcag gtgcgatcac catgtctgtg cgggatgcag ccaaaggcgt cattcacaaa
     1321 gggcaggtca gtgacgggtt gttaaacaag gtggagatgg cgttccgtgc gtatgacccc
     1381 tgtttcgggt gtgcgaccca taccctccca gggggattgc cgatgacggt gaatatctac
     1441 gatcatgaga agaaattata taaaacattt tcacaacctt aggagtatga gatgaaaaca
     1501 atcgtgcttg gtataggcaa cccgatccta caggatgatg gcgtagggct tcatgttatc
     1561 gatacgcttc gccagcagat gaacaacccg ctggtgacat tagaaaccgc atcgaccggt
     1621 ggaatgaacc tgctggatat gatccgtggg tacgaaaaag tcatccttat cgatgccgtg
     1681 aaacaaaaag agagtgtacc aggtgaggtg aaacggtttt tactatcgga ttttcacacg
     1741 gttcattcct gtaatcctca tgacgtctct ctttcagaag cgttgcattt ggcaaaacaa
     1801 ctcggggaaa aacacctccc gtcaaagatc atcctgattg gtattaccgt gaacaatacc
     1861 tatgagtttg gggaacatct cagcagcgag gttgcctctg cggtacccac cgcggtcaac
     1921 ctggttctgg aggagttgaa aaacacctag aggagaaaag catgtcgaaa gaatttgaac
     1981 caaacatcct gggatttctc tgcaactggt gcagttacgc gggagcagat ctcgcaggta
     2041 ccagtcggat gaagtatcca tctaacctca aatcaatccg ggtcatgtgc tccgggcggg
     2101 tagacccagc ttttgtgctt gaggcattac ggaaaggagt cgatggaatc cttatagcag
     2161 gatgtcatcc tggtgactgc cattaccaat cagggaatta taagacaaat agaaggatta
     2221 aactcctcaa aaaattatta gaggaacttg ggattgatcc gcaacgggta cgctttgaat
     2281 acgtctctgc atctgaaggt cagaagtttg caaccatcgt cacagagttt gtcgccgaga
     2341 ttaaaaaact aggaccaaat cccattacgg ttgaaaagaa ttaaaagaga aaaaaaaggt
     2401 gaggtatcca tggatgaaaa gaagaaagga tctgttgaag aggcagctga aaaaaccggg
     2461 gaagtcattg gcaagggcgt aaaaaaagga tggggcgccg tcaaatcatt tgggaaagga
     2521 gtgaaagaag gcgtatccaa ggagaaaaaa gaagaagaag aaaaaaaata aaacgatcag
     2581 aagagatcgt tcgtacttat tcaagaacgt gaatgttttt ctttgcgcag atttccttga
     2641 gttcctttgg ccagacagtg acgctgactt caccaaggtg tgctttcttc aacaagtaca
     2701 tgtaggttcg tgattgtccg atgccaccac cgatgcttaa gggtatttta ttgttcagga
     2761 tcgcctgatg gtacgggagg ttaaggaaat cagtttgatc ggagaactcc agttgttttt
     2821 tcaacgtctc aggggtgacc cggatgccca ttgaggagag ctcatgtcgt cgttgcgtca
     2881 ccgggttcca gacgaggata tccccgttta acccatgggt ctgtttccct gtttcttttg
     2941 acgtgtctgt gatccaatca tcataatcgg cagcacgcat ctcgtgagga tatccatctt
     3001 ccaacacgga accaatcccg atgatgaaaa tcgcagggtg atcctgcagg atagcggtct
     3061 ctcgctgctt ccgaggcagc tcaggataca tattcaggat ttcttcagca tggaagaact
     3121 caagtttgtt gggaagattg ggaatcgttt tagattcgag ctgtggaaag tcatcaagta
     3181 tgagggtttc agctcctttg aaaaccttcc agattttatt gactgtctct ttcaggtatg
     3241 aaatagtacg ttgatccttt gtgatgactt gctcccagtc ccattgatcc acataggagc
     3301 tgtggtcatg atcaagaaaa taatctttgc ggaccgcacg catgtcggtg ttcaaaccct
     3361 caccgacttt catatcaaat tctttcaatg ccatgcgttt ccattttgtg gctgcctgta
     3421 cgacttgtgc ttgcatgcga ggttgaatac cgaggccaca gggaaattca acaggtgttc
     3481 gggagccatc tcggtcaagg taatcgttca caccactttc cttatctacg ataagcggaa
     3541 cggtcaccat catgagattc agctctgttc ccaagtgttc ctcgatatag ttctttgctg
     3601 aaaaaatcgc tttctgcgtt tcctttggtg tcagcaacga tttatagttt tttggaagga
     3661 ctttttcgac ttctttatag gtgctaatac ctggacccgc tagatctgct ttcttattgg
     3721 ctgtcatggc tatcaaacct tcttccaggc aaaacagaag agatatttaa accttttatc
     3781 accactacga tgaggagtgt tatggaaaaa tcataaaaga atcggagagg tttgatgtta
     3841 ttggttgctt tattatccta ttgcaagtat ggttactttt actacgagtc gttttcctgc
     3901 taagagatga ttcatatcca atgtcaatgt ctgatcatcc atcgcctgta tcgttccgat
     3961 aaacgattta ccagttggca catcgatttt tatccgagaa ccgacctcta aggtcacctc
     4021 ggttttgaac actgattttg gtactttaat gacgaggtca tcgatgtggg gaccaaatgc
     4081 gtcttcagct tcaagggtta ttacctttgt ttctccctcg gtcatctcat gcaatgcttc
     4141 ttcgacatgc gggaaaaatt ttccgtctcc gacgacaagt tcaagtggat tttgtgcatc
     4201 gttcgtgtag cagagctcac cggactctaa tttggcttca cataaaagct tgaccgtatc
     4261 cccttcctta acgttcttca tggtctctcc tttcaaacaa ctcatcccga caaacaaaaa
     4321 acacacacta cttgagatat tggtgcatca atttcatccg tagggtgaaa aatcctatct
     4381 cattatggtt cttaaacatt tgtttttttt tatgtgaatt aatgaggaca taaacctgtg
     4441 gggcaatccc ccgcgtagtc agttgtcacc acgattggtt tgcctgtccc tagatatagc
     4501 acctgttctg gtttactccc atatcggtac acggtgattc ctttgcattt tagcttccat
     4561 gccagcaggt agatatttct tacatccttt tgtgtggaat tctttgggag attgactgtt
     4621 ttactgactg cgttatcaac gtatcgttgg aacgcagcct gcatctgaac gtggaattct
     4681 gaggtgatat ccagtgcggt gatgaagatt tttttcaggt cgtcaggtag ttttaagcgc
     4741 ttgatattcc cggttttcga tatctgacga accagttcct cggaatacag attgcgacgt
     4801 atcagttctc gcttgaaaat cccgtttatc tctggtagtt gttttccttc catgactcgt
     4861 ctgatgaatg ctaatgcgaa cacaggttcg attcctgagg aacagccagc tatgatgctg
     4921 atggtcccgg tgggtgcgat ggtgatgcag gtcgcattcc gcatcgcggg gtattttttc
     4981 tgccaggtac ttttggtaaa attcttaaac gaccctcgtt ctttaccgag ttctaccgat
     5041 ttttctgttg cttctttggc gatgaacttg atgagtcggc tgccaagctg caacgcgtcc
     5101 ttgctttcgt aggggatctt caacatgaat aacaaatcag caaatcccat cacacctaga
     5161 ccgattttcc gattggcttt tgtaatgttc tctgtctctt ttaatggata attgttcgca
     5221 tcaatgacgt tatcgagaaa atggactgca agtcgcgtcg tctgccttaa ttttttccaa
     5281 ttcagttttc cgttggttat catccgcgca aggttaatgc tgccgaggtt gcatgattca
     5341 taggcgagta acgggacttc gccacagggg tttgtcgttt caatgatacc catgtctcgg
     5401 aggggatgtt ttcggtttat ttc
//
LOCUS       DATMXJ010000005         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_66, whole genome shotgun sequence.
ACCESSION   DATMXJ010000005 DATMXJ010000000
VERSION     DATMXJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VN377_00155"
     CDS             complement(<1..350)
                     /locus_tag="VN377_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formiminotransferase"
                     /protein_id="HWR26827.1"
                     /translation="MARLIECVPNFSEGKNKHTIDTIHTLIQSHPEVTLLDFTPDADH
                     NRTDVTLIGSPEAIKTVALEIALKCVELIDMNTHKGEHPRMGAIDVVPFIPITEVDMS
                     ECVQLANDFAKEFS"
     gene            409..1626
                     /gene="hutI"
                     /locus_tag="VN377_00160"
     CDS             409..1626
                     /gene="hutI"
                     /locus_tag="VN377_00160"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWR26828.1"
                     /translation="MESIDLLITHASEMLTLKGSKKPRIREQMKNLGIMKNGSIAIQN
                     GQIVDVGRNLRYKAETVIDASGKLVMPGFVDPHTHVVFAGSRESELGLKLAGVPYMEI
                     LRRGGGIFYTVEKTRKASPTQLLQQSKKRLDTMLRYGTTSCEAKTGYGLDVKNELKLL
                     RVQKRLQETHSMDLVSTFLGAHAIPKHTQANDYIQTILTDMLPKTKGLAQFCDVFCEK
                     GVFTVAQAKKILEAGKQYGLIPKIHADEIVDTGGASLAAEVGAMSADHLLRSSEAGLR
                     AMAQKHVIGVLLPGTPFCLMMQQYAPARKIIECGVPVALATDLNPNCWTENMQLIIQL
                     ACLKMQMTPAEAITAATFNAACAIGMQHTIGSLEKGKQADVLILDCPNHQFLPYHFGI
                     NLVETVIKKGHVI"
     gene            1725..>2946
                     /locus_tag="VN377_00165"
     CDS             1725..>2946
                     /locus_tag="VN377_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWR26829.1"
                     /translation="MKTRTIRVSFLLGIFLLSLLPLHVPAAQDGWWDDAWSFRQEIIL
                     DSITSKESAANQPVDTTIRFDSPCWARNETYHSVRVIFQNKDDDIELESQLYNMKYSD
                     ETHITSCNIVFLIPPQTDGTERYYIYYDESPTTSPDYPDHVSIADSSYFYEPIPGYPL
                     ESHFYKISQQDTIRYVVAQEGQFLWYSTSQYVTKLQEGSTDVMPKNGEAIASFEYSYY
                     YGAEMWQFNSTSQELITKEILCDGNLMVSCKILSRSLGDDLQTTAIYKYYYCPTSSER
                     IQVHVIHETLKEYQVYAESNTDGTYASLQCGGMRSTSIADLNFGEIYPYFHLYSEQNT
                     VEEYRVDLHPEYTQENPVLWLIQTPDDVDMGKKPWVSFDEGITGNVHALIFGSSSVVK
                     AGKDERDGIQLKAYE"
BASE COUNT          821 a          642 c          683 g          800 t
ORIGIN      
        1 tctgagaatt cctttgcaaa atcgttggca agctgtacac attcgctcat gtcaacctcc
       61 gtgattggga tgaacgggac gacatcgatt gctcccatgc gcgggtgctc tcccttgtgt
      121 gtattcatat cgatgagttc gacgcatttc agagcgattt ctagtgctac agtcttgatt
      181 gcttccggag aacctatgag ggtgacatcg gttcggttat ggtctgcatc aggggtaaaa
      241 tctagcaatg tgacctctgg atgactctgt atcaaggtat gaatcgtatc aatcgtgtgt
      301 ttattttttc cttcactgaa atttggaaca cattctatga gccgtgccat aggtggtcac
      361 cgtaaacctt aatcaagagt ccttcattta actatttggg aacatcctat ggaatccatc
      421 gatctcctta ttacccatgc aagtgagatg cttacgttaa agggctcaaa aaaaccacgg
      481 attcgggaac agatgaagaa ccttggtatc atgaaaaatg gatcaatcgc gatacagaat
      541 ggtcagattg ttgatgttgg tcggaatctc cggtacaagg cagagactgt gattgatgcg
      601 tcaggaaaac ttgttatgcc tgggtttgtc gatcctcata cccatgtcgt attcgcaggg
      661 tctcgtgagt ctgaactggg cttgaagctc gctggtgtgc cgtacatgga gattcttaga
      721 cgtggtggag ggatttttta tactgttgag aagacccgga aagcctctcc aacccagttg
      781 ctgcagcaaa gcaagaaacg tcttgatacg atgcttcgat atggtactac ttcgtgcgag
      841 gcgaaaaccg ggtatgggct tgatgtgaaa aacgagctga agctgttaag ggttcagaag
      901 agactgcagg agactcattc catggatctt gtttcgacgt ttcttggtgc gcatgcgatc
      961 ccaaaacaca cacaggcaaa cgattacatt caaacgattc taacagacat gcttccgaaa
     1021 accaaggggc tcgcgcagtt ctgcgatgtg ttttgtgaaa aaggtgtttt tacggttgca
     1081 caagcaaaga agatccttga agcaggaaaa cagtacggtc taatccccaa gattcatgca
     1141 gatgagatcg ttgacaccgg gggggcatca ctcgcagcag aggttggtgc gatgagcgca
     1201 gatcacctgt tacgatcctc ggaggctggt ttacgtgcaa tggcgcaaaa acacgtcatc
     1261 ggggtgcttc tccctggaac tcccttctgt ttgatgatgc agcagtacgc ccccgcacga
     1321 aagattattg agtgtggtgt tccggttgcg ttagcaactg atctgaaccc gaattgttgg
     1381 acagaaaata tgcagctgat tattcaactc gcttgtctga agatgcagat gacccccgct
     1441 gaagcgatca ctgcagcgac gtttaacgct gcctgcgcca tcgggatgca acataccatc
     1501 ggaagtcttg agaaaggcaa acaagctgat gttttaatcc ttgattgtcc gaaccatcag
     1561 ttcctcccct atcatttcgg gataaatctg gtcgaaaccg tgataaaaaa aggccatgta
     1621 atttgataac cctatgtttt tttccttgca caaagaataa tataatacat gatgataatt
     1681 atctgtagat aaatatatta aaagtggggg gtctgttcct cggtatgaag actaggacga
     1741 tacgtgtttc attccttcta gggatttttc ttctctccct actgcctctc cacgtgcctg
     1801 cagcacagga tggatggtgg gatgatgcct ggtcttttcg ccaagaaatt atacttgatt
     1861 ctatcactag caaggaatct gcagccaacc agcctgtcga taccacgatt cgatttgatt
     1921 ccccctgttg ggcgagaaac gaaacctatc attctgtgcg ggtaatcttc caaaacaaag
     1981 atgatgacat tgaactcgaa tcccagctct ataatatgaa atattcagat gagacccata
     2041 tcacctcctg taatattgtg tttctgattc ccccacagac tgacggaacc gagcgatatt
     2101 atatctatta cgatgaatca ccaaccactt ctccggatta ccctgatcat gtgtctatcg
     2161 cggattcctc ctatttctat gaaccgatac ccgggtatcc tttggaatcc catttttata
     2221 aaatatcaca acaagataca attcgctatg tcgtagcaca agaaggacaa ttcctttggt
     2281 actccacatc acaatatgtg acaaaacttc aggaaggatc aacagatgtg atgccaaaaa
     2341 acggggaagc gatcgcgtcg ttcgagtact cctattatta cggggccgaa atgtggcaat
     2401 ttaattcaac gagtcaagaa ttaattacga aagagattct ctgtgacggg aatctcatgg
     2461 tctcctgtaa aatactcagc agatccctag gagatgatct tcagaccact gcaatctaca
     2521 aatattatta ttgcccaacg tcctcagaac gaattcaggt ccatgtgatc catgagacgt
     2581 tgaaagaata ccaggtgtat gccgagtcga acaccgatgg aacatatgcc tcgttgcagt
     2641 gtggtgggat gcgaagcaca tcaattgcgg atttgaactt cggggagatc tacccctatt
     2701 ttcacctcta ttcagaacaa aatactgtcg aggaatatcg agtggattta catcccgaat
     2761 atacacaaga aaatccggtg ctttggttga tacaaacacc agatgatgtc gatatgggaa
     2821 aaaaaccatg ggtctcgttt gatgagggaa tcacagggaa cgttcatgca ttaatctttg
     2881 gctcttcttc tgtagtcaaa gcaggaaaag atgagcgtga tgggatacaa ttaaaggcgt
     2941 acgagt
//
LOCUS       DATMXJ010000006         6552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_62812, whole genome shotgun sequence.
ACCESSION   DATMXJ010000006 DATMXJ010000000
VERSION     DATMXJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62812"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1268
                     /locus_tag="VN377_00170"
     CDS             288..1268
                     /locus_tag="VN377_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26830.1"
                     /translation="MKKVWIIFVCSLIMLNIFTGLISTGSILIAPPTPPQQPPIGPGG
                     SNYSHSGVIVHRYGVDVLQYWLFEPADPTPATAPLIVFNHGYGAIFPFWYQTWINHIV
                     RRGNIVVYPRYQQTWLPLGVFHFYSNAITAVKNALHELEQGDHVKPELDHFAIVGHSY
                     GGGITCDMAVLAESEGIPVPKAIMPIQAVAWMTNLSLIPGSILMIVIYGEDDPLIKKW
                     MEKQIFYGAENIPLENKDFVTQVTDTYGKPHLIADHSAPLTPTIPLAGFGKTDAMDYY
                     STWKLFDALTDYAFYGINREYCLGNTSEQRFMGLWSDGTPVKELQVTDSP"
     gene            complement(1512..1958)
                     /locus_tag="VN377_00175"
     CDS             complement(1512..1958)
                     /locus_tag="VN377_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26831.1"
                     /translation="MDKSRVTMLSLFSGFTFSAITLLLDQLPDPSSFISQLTLFFLVV
                     LFDLCLFLLAWQTIIMIGTWNVSKVPAHAKWELSVFNLLLMIVFILWGWSVVLMFLLR
                     NLTFLTLVSGGLWVAVIVSAVVVLRGMVKRLGWSVKKELKHIRDNQ"
     gene            complement(2036..2425)
                     /locus_tag="VN377_00180"
     CDS             complement(2036..2425)
                     /locus_tag="VN377_00180"
                     /inference="COORDINATES: protein motif:HMM:NF039352.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6114 domain-containing protein"
                     /protein_id="HWR26832.1"
                     /translation="MSKKLVIGSEKPPKDFILSLIAGILIVSNTVLLGVAARWFPGMI
                     PTLPGSANDTTVLYRLTALGLIFGVLVLLGAIMLHIRPVNKKAWGIMILVFSIPSVVT
                     GGGFIIGFILGIIGGAKALLRKSNVQA"
     gene            complement(2603..3514)
                     /locus_tag="VN377_00185"
     CDS             complement(2603..3514)
                     /locus_tag="VN377_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR26833.1"
                     /translation="MSRTLVTQKYLAVGIILLFVGTCISPSTAYTMEKTQSTSRGNRM
                     YVGGSGLENYTRIQDAIDNASEGDTVFVYNGWYYENIVINKSIIVSGEDRNTTFILGE
                     NDSDIVRMTECSAVFTGFTIQEYHEGGFSGIYISDCWSSQISQNNVISCENGILVANS
                     ESILVSNNLIQNCSFGILEVITGNITVTENRIEGNGKGYGIWIEATMFKNYITRNSIT
                     NNTVGIFLFFTMHSMIQENNFLDNQQQAFFANSFFTKWQQNYWNRARLLPKVIIGGIG
                     STLIIHKMIPFFNVDWKPAQEPYDIQG"
     gene            complement(3589..4107)
                     /locus_tag="VN377_00190"
     CDS             complement(3589..4107)
                     /locus_tag="VN377_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWR26834.1"
                     /translation="MELKEVIRNRHSVRSFSPTPIPKTILDEILFSANQAPSAGNLQA
                     RDFIIIDDQRIKEQLCAAALDQAFLTEVPIIIVVCSNQKRITPYGTRGKDLYCIQDAS
                     AAVEHILLAAVDNGLDACWVGAFDEQTVSKILQVPSYIRPVALIPLGYSKKKGTASRR
                     MDIKNLVHFNHW"
     gene            complement(4271..6124)
                     /locus_tag="VN377_00195"
     CDS             complement(4271..6124)
                     /locus_tag="VN377_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR26835.1"
                     /translation="MLLLLVLDCVVPNVSTIEENKGDDEVELWEYLNDWSVHSDDSNS
                     RIEPICSIVQSGESVVNRNHQGFEPIDGPMNSPWPMFGHDVIHTCRSTISTINITGTE
                     IWRKCENRSGSFWGSPLIDNNNIIYFGACADSALNAFYPNGTRKWRYISDGSVWATPA
                     IAEDGTIIFPTWGGYSTVHAVYPNGTVKWTYRDGYEAHSLSSPAIGNDGTIYFGTDHY
                     PNYNIYAINANGTLQWKYSTGFITNGAPAIGSDGTIYIGSGDHHLYAMNPNGTLRWRF
                     DAGSDIKGAATIVSDGTIYIPAFNGYFYALAPNGMLLWKGYTGDSVAAAGLALAIDGT
                     IYIGTEKLRAYNPNGSLKWDIELPGDVYGSVPAVSSDGTIYVSAGSCLVAINPDGTER
                     WRKEIANEQIRSSPCIGPGDRIYVGSNEHYSNNWNLHAFGLGPLKTEAQGPYQGIATY
                     SLKFTGIAFGGTLPYQYHWDFGDGNTSDELAPTYIYSFAGNYSATFTVIDGDGNSSND
                     TATVHIDKAPFTVSFTKPKKGLYINDKRIIPLPNKVYAIGPITFEVLVSEPEIERVEF
                     YIDDILMSTDTEQPYSWTWDTMTFSKHHVAVYAYDKTGQEGSTGFLMSKFF"
     gene            complement(6255..>6552)
                     /locus_tag="VN377_00200"
     CDS             complement(6255..>6552)
                     /locus_tag="VN377_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HWR26836.1"
                     /translation="VNDEVDALLTPTGNTPLYTDLKNLFAEVLHKQYQKNEYVKQFTT
                     RIPENLEKIQRMETIFRTRILDTPEVVFTVLKDQQKRLLDAQRTYGDYISPEQW"
BASE COUNT         1843 a         1392 c         1284 g         2033 t
ORIGIN      
        1 ggagtatcag tatcttgtcg gtttttttat attgcacttt tttatgatga ttccaccaag
       61 tgccatagat gatattcttg atattcttta ctctctttta tatccttggc taacattata
      121 cgaatttttt cattgctcca ccaccaaatt ttcccactaa aaaattccca tcatcatatt
      181 acccagtact aaaatcagaa tcttaaaacc gtgcgaatac ttctgattct aaagatatgt
      241 ttttaaagga gaacagtgtt ataaatttgt atattggagg aaatgttatg aaaaaggtat
      301 ggattatctt tgtttgttca ctgattatgc tcaatatatt tacgggactg atttcaacag
      361 gttctatact aattgctcct ccgacgccac cccaacaacc tccaatcgga cctggaggat
      421 caaattattc ccattcaggg gttatcgtcc atagatatgg agtagatgtt ctgcagtatt
      481 ggctctttga acctgctgat ccaacaccag cgactgcccc cttaatcgtt tttaatcatg
      541 gctatggagc aatatttccg ttttggtatc aaacatggat taatcatatc gtgagacgag
      601 gcaatatcgt ggtgtatcct cgatatcaac aaacgtggtt accactcgga gtttttcatt
      661 tttattcaaa tgctataaca gcggtaaaaa atgctcttca tgaattagaa caaggggatc
      721 atgtgaaacc tgaacttgat cattttgcta ttgtagggca ttcttatggt ggtggtatca
      781 cttgtgatat ggctgtgttg gctgaatctg agggaatacc tgtcccaaaa gcaattatgc
      841 cgatacaagc agtggcttgg atgactaatc ttagtttaat acctggttca atcctgatga
      901 tagtaattta tggggaagat gatccactca taaagaaatg gatggaaaaa cagatttttt
      961 atggagcaga gaatataccc ttagaaaaca aggacttcgt gactcaggtt accgatacat
     1021 atggtaaacc tcatcttatt gctgatcatt ctgctcccct tactccgacg attccattag
     1081 ccgggtttgg aaaaactgat gcgatggatt attattctac ctggaaactg tttgatgcac
     1141 tcaccgatta tgctttttat gggataaaca gggaatattg tcttgggaat acttcagagc
     1201 aacggttcat gggtctctgg agcgatggaa caccagtgaa ggagttacaa gtcactgatt
     1261 ctccttaagt tgtcctagat atcaacagcg cttgttttac catcggcctc gtccgtcctc
     1321 caccaggaat cgtaggttta aaaggaataa aaatatcaac tagcgtaaat agttgcccgt
     1381 tcttagggaa ttgtctatat gtaaaaatga gttcggaacc gtctaggaaa ctttgagttt
     1441 ttttctaatt caatgtggct gtcagccttc atctattgtc gtattaacct ggtgaaatat
     1501 acgacatact ctcattgatt atctctgata tgctttagtt cctttttgac tgaccatcct
     1561 aagcgtttga ccatgccgcg taatactact actgcgctga caatgaccgc cacccataat
     1621 ccacctgaca ccagcgtgag gaaggtcaga ttccgtaaca gaaacatcaa cactactgac
     1681 caaccccaca aaatgaaaac gatcatcaac aacaaattga aaacggacaa ctcccacttc
     1741 gcatgtgctg gtaccttact tacgttccac gtaccaatca tgattatggt ttgccatgct
     1801 aacaagaaga ggcataagtc gaataagaca actaagaaga agagagtgag ttgtgagatg
     1861 aaactacttg ggtctggaag ctggtcaagc agtagcgtta tagcactgaa tgtaaaacct
     1921 gaaaaaagag acaacatggt gacacgcgat ttatccaaag ccttgacata tcccgtgtat
     1981 tcctcttcgt tttcattcat tttaggtcca cctgaagacg tgtatgtcaa attctttaag
     2041 cttgcacatt tgatttccgt aagagtgctt ttgctccacc tattataccc aatataaaac
     2101 ctattatgaa tccgccaccc gtgactacac tgggtatcga gaacacgagt atcatgatgc
     2161 cccacgcttt cttgtttacc ggtctaatgt gtagcattat ggctccaagt aatacgagaa
     2221 caccaaatat caggccaaga gctgtaagac gatagagaac cgttgtgtca ttggctgacc
     2281 ctggaagcgt cggtatcatt ccaggaaacc acctcgctgc aactcccagg agcactgtat
     2341 tgcttactat gagtatcccc gcgatcagag acaaaataaa atctttaggt ggtttttcgc
     2401 ttcctattac taattttttg ctcattcaat taccttcaga tatcaataac agaatgaatt
     2461 tgatttctga tattcccgat tcgggtgttg tgtgaaatca gtcaaaggtc ttaatggttt
     2521 tggtgattga taaaatcatc ctctgaaaaa aaaataatgg tgagcgttaa tctttcctcg
     2581 ttccaacagt tattgtggtt ctttatcctt ggatgtcata tggttcttgg gcaggcttcc
     2641 agtcgacatt aaaaaacggg atcatcttat gaataatcaa tgtgctgcct atcccaccga
     2701 tgattacctt gggtaagaga cgagcccggt tccagtaatt ctgctgccat ttggtaaaaa
     2761 aagaatttgc aaagaatgcc tgttgttgat tgtctaggaa gttgttctcc tgaatcatgg
     2821 aatgcattgt gaaaaaaagg aagatcccca ccgtattatt cgtgatactg tttcttgtga
     2881 tataattctt aaacattgtt gcctcaatcc aaatcccgta tccttttccg ttcccttcta
     2941 tcctgttctc cgttaccgtg atgttcccgg tgataacctc caaaatacca aaagaacagt
     3001 tctggatgag attatttgag acaaggattg attcggaatt ggctacgagg atgccgttct
     3061 cacaggagat cacgttgttc tggctgattt gacttgacca gcaatcactg atatagatac
     3121 cggaaaaacc cccttcatgg tattcttgaa tggtgaatcc tgtgaacacg gcgctacatt
     3181 ctgtcatccg tacgatatct gaatcgtttt ctcccaggat gaacgtcgtg tttctatctt
     3241 caccggatac gataattgat ttattgatca caatattttc gtagtaccat ccgttgtaga
     3301 caaaaacagt gtctccttca cttgcattgt ctatcgcatc ctgtatccgt gtatagttct
     3361 ctagtccact cccacccaca tacatcctgt tgcctcgtga tgtcgactgt gttttctcca
     3421 tagtatatgc cgtggatgga ctaatgcagg ttccaacaaa caacagaatg attccaactg
     3481 ccaagtattt ctgcgtcact agagttctac tcaagatttt gtacccctta atatattaat
     3541 ataagactca tgctttaaaa atatatctga gattctgttt tgtttttttt accaatgatt
     3601 gaaatgaacg agattcttga tatccattct tctggacgct gtcccttttt tctttgaata
     3661 tccaagggga atcagagcaa cgggtctgat ataggaaggc acctgtaata tttttgaaac
     3721 agtctgctca tcaaaagcac ccacccaaca ggcgtctaaa ccgttatcta ccgcggcaag
     3781 aagtatgtgt tcaaccgcag cggatgcgtc ttggatgcag tacagatcct tccccctcgt
     3841 tccatacggg gtgatgcgtt tctgatttga gcagacgaca ataatgattg gtacttcggt
     3901 aagaaaagct tgatccaaag cagctgcaca gagctgttct tttatccttt gatcatcgat
     3961 gatgatgaaa tctcttgcct ggagattccc tgcagatgga gcctgatttg cggagaagag
     4021 gatttcatca agtattgttt ttgggatggg tgttggagaa aagcttcgga cgctgtgacg
     4081 atttcgaatg acttctttga gttccatggg aatcatcgtt gaaaacacct ttttcttaat
     4141 aaacttattt tacgggtgat ctccttgttt cttttcgcct tggacaaaaa aatctataaa
     4201 taggctttgc acacaaatag ggaggttata tgcagaataa aaatttgaac ataaaatcca
     4261 ttttttgatc ttaaaagaat ttggacatca agaaacctgt acttccctct tgtcctgttt
     4321 tatcatatgc atagacagct acatgatgct tggagaacgt catagtgtcc caggtccaag
     4381 aatagggttg ttcggtatcg gttgacatta aaatatcatc aatataaaat tcaacacgtt
     4441 caatctctgg ctcgcttacc aggacctcga atgtaatagg tccgattgca taaaccttgt
     4501 ttggtaaggg gatgattcgc ttatcattga tatagagccc tttctttggt ttcgtaaaag
     4561 atacggtaaa aggtgcctta tcaatgtgaa ctgtggcggt atcattgcta ctatttccat
     4621 caccatcaat taccgtgaag gtcgcggaat aatttcctgc gaaggaataa atataggttg
     4681 gagcgagttc atcactggta ttcccgtctc cgaaatccca atgatattga tagggtaacg
     4741 tacctccaaa ggcaattcct gtgaatttta ggctgtaagt ggctatcccc tgatagggtc
     4801 cttgtgcttc agttttcaag ggaccaagac cgaaggcgtg taaattccaa ttatttgaat
     4861 aatgttcatt ggatccaaca taaatcctat cacctggacc gatgcagggt gatgaacgaa
     4921 tctgctcatt agcgatttcc tttctccagc gttcagtacc gtcgggatta atagctacaa
     4981 gacaacttcc tgcactcaca tatatcgttc catctgaaga gactgcaggg actgaaccat
     5041 aaacatctcc agggagttct atatcccatt tgagtgatcc attcgggttg tatgcacgca
     5101 gcttttctgt gccaatataa atcgtaccat ctatcgcaag ggcgagccct gctgctgcca
     5161 cgctatctcc tgtatagcct ttccagagaa gcatgccatt cggagccaag gcatagaaat
     5221 agccattaaa agcagggatg taaatggtgc catccgagac tatggtcgct gctcctttga
     5281 tatctgaacc tgcgtcaaac cgccagcgga gcgtcccatt aggattcata gcatataagt
     5341 ggtggtcgcc agaaccgata tatattgtgc catcagaacc aatcgcagga gcaccattcg
     5401 tgataaaacc agtgctatat ttccattgta gtgtaccatt agcatttatc gcatagatat
     5461 tataatttgg ataatggtcg gttccaaaat aaatagttcc atcgtttcca attgctggtg
     5521 aagatagtga atgagcttca taaccatctc gatacgtcca ttttactgta ccattcggat
     5581 atactgcatg gacagtgctg taaccacccc aagttgggaa gatgattgtg ccatcctcgg
     5641 caatcgctgg tgtcgcccaa accgatccat ctgagatata cctccatttt cttgttccat
     5701 taggataaaa tgcgttcaag gcgctatcgg cacaagctcc aaaataaatt atattattat
     5761 tgtcgatgag aggtgacccc caaaacgatc ctgacctatt ttcacatttt cgccaaatct
     5821 cagttcctgt gatgtttatt gtgctaatgg tgcttcggca ggtatgaatc acatcatgtc
     5881 caaacatcgg ccaaggagaa ttcattggac catcaatagg ctcgaatcct tgatgatttc
     5941 tatttacgac cgattctcca ctttgaacaa ttgaacaaat aggctctatc ctactatttg
     6001 aatcgtcaga atggacagac caatcattca aatattccca aagttctacc tcatcatcac
     6061 ctttgttttc ttcaatagtt gatacattag gaacaacaca atcaagaaca agtaacagta
     6121 acaatccaac cgcaagagct ttctgtatca gatgattttc ctcctttaca atttaataac
     6181 aatgttctcc tttaataaca tatccttaga ttttatttat agtgcgatat agtttttttt
     6241 tattctaccg cgattcacca ttgctcaggt gaaatataat ctccataggt acgttgtgcg
     6301 tcgagcaatc gtttctgctg gtctttcaga actgtaaaga cgacctctgg agtatcaagg
     6361 atcctcgtgc ggaagatcgt ttccattctc tgtatcttct caaggttctc agggattcgt
     6421 gtcgtaaatt gcttgacgta ctcatttttc tgatactgtt tatgaagcac ctctgcaaac
     6481 aagtttttca ggtctgtata gagcggggta ttccctgttg gtgtaaggag cgcatcaacc
     6541 tcgtcattta ct
//
LOCUS       DATMXJ010000007         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36684, whole genome shotgun sequence.
ACCESSION   DATMXJ010000007 DATMXJ010000000
VERSION     DATMXJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36684"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VN377_00205"
     CDS             complement(<1..452)
                     /locus_tag="VN377_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWR26837.1"
                     /translation="MIEKHDIEACFIELAWIQDKSLRNKVVNVWKNAADRGKWQRLQE
                     IPFTLIFENSGLLVDHTKRITALVRNVGNTRQEILNKDYLIAGALLHDVGKLLEYELK
                     DGKILKSAFGEKTRHPAAGAQLADECGLPKEVVHIIAAHSHEGDTMNRT"
     gene            595..1887
                     /locus_tag="VN377_00210"
     CDS             595..1887
                     /locus_tag="VN377_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26838.1"
                     /translation="MKFKKIDTIIIVVLIVSTTLFLVKADYISMILPNVHTNPPGKNG
                     TTVTPLPVEQIPAPPTSLVPAYRRDVSPEDEGVHFNELRTSREWWYFNAVFTDGSSQL
                     KDWAVAVSFNHMARGDLFGTNKPDLLVVSILGNNSETYGGMVNKEHHLGILKDGTLIA
                     GSPGVKVEFEDSWAEGSYPRWHVHVQNPDVDAAHEIMIDLDFTAKSLPVWALGSHVIE
                     KSDSEVANYVILGCDVSGTITIDGKTYAVKGTGSHEHSWTPNAIRKLFIGGWDWFTLT
                     LDNGWDIYAANYLPSPQAISGALSSLDPFGTLLITTDRGETITELKDVNLKITQRDEK
                     VFPLVRLPSAFSIKADPSINPLYLVSQSLLYGTKTTLNGDISITHATNQIWKFPSYLG
                     MKIGYCVIDGTVSWTDDKGDHQVPIHGIGVSWNMRALL"
     gene            complement(2021..2407)
                     /locus_tag="VN377_00215"
     CDS             complement(2021..2407)
                     /locus_tag="VN377_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HWR26839.1"
                     /translation="MKGRTISPGKVKGIALVSKEPIGFYGGINIKTGIVIEKGHPLEG
                     KNVKDRILVFPCGKGSTVGSYVIYGLQKNGVAPKGIINKETETIVATGVILAGIPCVD
                     KIDIDQIKDGDTVVLNADNATVEVAK"
     gene            complement(2407..3591)
                     /locus_tag="VN377_00220"
     CDS             complement(2407..3591)
                     /locus_tag="VN377_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HWR26840.1"
                     /translation="MYLSKEEEKILHGEQGEVPERMLRLLTQLGDIYGADKMIPVGSV
                     QVAGVSYKSIADPGTEFLEDLAGKGAKVKVLTFLNPAGMDLENWETLKFPKEFAKNQL
                     RIMDAFKSMGIVITATCTPYLAGNLPRFREHIAWSESSAISFSNSVIGARTNREGGPS
                     ALAAAILGKTPNYGFHLWENRQPSIKVKIETDLTFDADFGALGYHVGKQVKNKIPYFT
                     GIKDADTDQLKSLGAAMAASGAVALYHVEGLTPEADLVKKDNLETITVTKKELDEAYA
                     KLNTGKEPDIVILGCPHASLREISAVAEKLKGKHLRKPVWVCTSRMMKEAAERMGYSE
                     IIEKAGGYIVADTCMVVSPIEDMGYKTTGVNSGKAGNYLPGFCKQNVCFANLDKLLEA
                     AL"
     gene            3655..4821
                     /gene="truD"
                     /locus_tag="VN377_00225"
     CDS             3655..4821
                     /gene="truD"
                     /locus_tag="VN377_00225"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HWR26841.1"
                     /translation="MKLKQLPADFVVEEIPDYPIVSEKDEHTIFLLQKQEVDTFDAIR
                     RIAQRLRISLFEIGYAGLKDKHALARQYVSIPTKYNVQGLKLDTVVLTLVGYSRKKIK
                     IGDLAGNRFTIIVRDIKDVELADISLRAETIATSGVPNYFDSQRFGSVINKVFIGKEL
                     ILKHYETAVKQYLTAYQKSEPKKIKDEKRRILSSWNDLAAVRVFNKAFALVMKEYLKT
                     NDWCAAYRKIPAHLREMYVNAYQSFLWNECVKEILKNTIEKKKLYSVEYAIGSLLFFT
                     TLSEQEHGRLPQTIQTISDQMTITDDERKVIDHVLAKEGLRLTDFRIEDETGNFFKTR
                     ARQVLLKAEDLTIGTSERDELNSKGNVERYKIQISFSLSKGSYATIMTKRLFGH"
BASE COUNT         1236 a         1029 c         1158 g         1402 t
ORIGIN      
        1 gtcctattca tagtatcgcc ttcatgagaa tgtgctgcga tgatatgtac gacttctttt
       61 ggaagaccac attcatcagc aagttgggca ccagccgcag gatggcgtgt tttctcccca
      121 aatgcacttt tgagaatttt cccatctttt agttcgtatt caaggagttt tccaacatcg
      181 tggagcaaag caccagcgat gagataatct ttattgagaa tctcttgtcg ggtattacca
      241 acatttcgaa ccagtgctgt tatccgtttg gtatggtcga caagcaaccc tgagttctcg
      301 aaaataagag taaaagggat ttcctggagt cgctgccatt ttcctcggtc ggcagcgttt
      361 ttccatacgt tcacgacctt gtttctgagg cttttatcct gtatccacgc aagttcaata
      421 aaacaagctt ctatatcgtg tttttctatc atacaatgcc tccgagccta gttggggtgg
      481 taattgtact gtattataat aatttgcgcg tagaggaaat gggtaccgat tgtaaagaaa
      541 actattaata ttctcctatc cgaagcacag tataacgata tcgtttggtg gagaatgaaa
      601 ttcaaaaaga ttgatacgat catcatcgtc gtactcattg tctctacgac actttttttg
      661 gtcaaggcag attatatcag tatgattctc ccaaatgttc ataccaatcc ccctgggaag
      721 aatggcacta cggtcactcc tctgccggta gaacagattc cagctccacc gacctctctt
      781 gttcccgcgt atcgccgtga tgtatcccct gaagatgaag gggttcattt caacgaactg
      841 cgtacctccc gggaatggtg gtattttaat gcagtgttta ctgatggttc gagtcagtta
      901 aaagattggg ctgttgcggt cagctttaac catatggctc gaggtgatct gtttgggacc
      961 aacaaacctg atttgctggt tgtcagcatc cttgggaaca acagtgaaac gtatggcggg
     1021 atggtcaaca aggagcatca tctcgggatt ctgaaagatg ggactctgat tgctggttct
     1081 cctggtgtga aagtcgaatt tgaggacagc tgggctgaag ggtcatatcc acgttggcat
     1141 gtccatgtcc aaaaccctga tgtcgacgcg gcacatgaga ttatgattga tcttgatttc
     1201 acagcaaagt cgcttcccgt ctgggctctt gggtcgcatg tgattgagaa atcagatagc
     1261 gaagtagcca attatgtaat acttggctgt gacgtgtctg ggacgattac catcgatggg
     1321 aagacctacg ctgttaaagg aactggttcc catgagcact cttggacgcc caatgcgatc
     1381 cgaaaactgt tcattggtgg ttgggattgg ttcacactga cactggataa cggctgggac
     1441 atctacgcag ccaattatct tccaagtcct caggcgatct caggtgcatt gtcgtctctt
     1501 gatccgtttg gaacgcttct cattaccact gaccgtggag aaaccattac cgagttgaag
     1561 gatgtgaatc tgaaaatcac gcaaagggat gaaaaggtgt tccctctggt gagactcccc
     1621 tctgcattca gcatcaaggc tgacccaagc attaatccac tgtatctggt cagtcaatcc
     1681 cttttgtatg ggacgaaaac gacattgaac ggtgatattt caattaccca tgcgacgaac
     1741 cagatctgga agttcccttc gtatctaggg atgaagatcg ggtattgtgt gattgatggg
     1801 acggtctcct ggactgatga taaaggtgat caccaggtgc cgattcatgg gatcggggta
     1861 tcatggaata tgcgtgcgct gctatagatt tttaaaaata gtgatagaat tgtcctgatt
     1921 gctactgatt tgaccgggtt attcttttta tactagatcc ttataagaaa cgaaaaggaa
     1981 agaacacaga ggttctctcg cataacggaa aaaattgaga ttattttgcg acttcaacag
     2041 tcgcattatc cgcatttaac accaccgtgt ctccgtcttt tatctggtcg atatcgatct
     2101 tatctacaca agggatacct gcgaggatga caccggttgc gacaatagtc tctgtttctt
     2161 tgttgatgat ccctttaggg gcgacaccat ttttttgtaa cccgtagatg acgtaagagc
     2221 cgaccgtgga tcctttcccg caggggaaca caagaatcct atctttaacg ttcttcccct
     2281 ccagtggatg gcctttttca atgacaatgc cggttttgat gttgatgccg ccatagaacc
     2341 cgatcggttc tttggaaact aaagcgatgc ctttcacttt ccccggtgag atggttcgtc
     2401 ctttcatcat agtgcagcct ccaggagttt atcgagattt gcgaaacaca cgttttgttt
     2461 gcagaaacca ggcaaatagt tacctgcttt gccggaattg acccctgtgg tcttgtaccc
     2521 catgtcttcg ataggtgaga cgaccatgca ggtgtccgcg acgatatacc ctcctgcttt
     2581 ttcaatgatt tctgagtatc ccatacgctc tgctgcttct ttcatcatcc gggaggtgca
     2641 cacccagact ggttttctaa gatgttttcc tttgagtttc tctgcgacag ccgatatctc
     2701 tcggagtgat gcatggggac agccgaggat gacaatgtct ggttctttgc cagtgttgag
     2761 tttcgcgtat gcctcgtcta gttctttttt tgtgactgtg atggtttcaa ggttgtcttt
     2821 tttcacgagg tctgcttctg gggtgagtcc ttcgacgtga tagagtgcaa ccgcacctga
     2881 cgctgccata gccgcgccga gtgatttaag ctggtcagtg tccgcgtctt taataccggt
     2941 gaagtacggg atcttgtttt tgacttgttt gccgacatga tagccaagag cgccgaagtc
     3001 agcatcgaag gtcagatctg tctctatctt tactttgatg gatggttgtc tgttttccca
     3061 cagatggaaa ccgtaatttg gtgttttccc aaggatcgcc gccgcgagag cagaaggtcc
     3121 tccttctctg ttggtccgtg caccaatcac tgagttagaa aatgagatcg ctgaagattc
     3181 tgaccaggcg atatgttcac ggaaccttgg gaggtttccc gcaagatatg gcgtgcaggt
     3241 cgcggtaatc acaataccca tacttttgaa cgcatccatg atgcggagct ggttttttgc
     3301 aaactctttt gggaatttca aagtctccca gttctccaag tccatgcctg ctgggttcag
     3361 gaacgtaaga acctttactt ttgctccttt tcctgcgagg tcctcaagaa actccgttcc
     3421 tgggtctgcg atggatttgt aggagacacc agcgacttgt actgagccga ctgggatcat
     3481 tttgtctgca ccgtagatgt caccaagttg ggtaagtaaa cgcagcatac gttcagggac
     3541 ttcaccctgt tcgccatgaa ggattttttc ttcctctttt gaaagataca tactgtttca
     3601 gcccctatgg acagggttat atgctctgat tattttagtg tttgtgtttt ccgtatgaaa
     3661 ttgaagcaac tgccagctga tttcgttgtc gaggagatcc cagattaccc gatagtatcg
     3721 gagaaggatg aacacaccat ttttttactg cagaaacagg aggtggatac gtttgatgcg
     3781 atccgtcgga tcgcgcagcg acttcgaatt tctctttttg agattgggta tgcgggactg
     3841 aaagacaagc atgcccttgc tcgacagtac gtttcgattc cgacgaagta taacgtgcag
     3901 ggtctgaagt tggatactgt ggttctaaca ttggttggat attctcggaa gaaaatcaag
     3961 ataggtgatc ttgctgggaa tcggtttacg attattgtcc gtgatataaa ggatgttgag
     4021 cttgcggata tttccctgcg ggctgagaca atcgctacat ctggtgtgcc gaattatttt
     4081 gattcacagc ggttcggcag tgtcattaat aaggtgttca ttggaaagga attgattctg
     4141 aaacattatg aaacagcagt caaacaatat ctcactgcgt atcagaaatc ggaaccgaag
     4201 aagatcaagg acgaaaaacg aaggatcctg tcatcctgga atgatcttgc ggctgtacga
     4261 gtgttcaaca aagcatttgc actggtgatg aaagaatatt taaagacaaa cgactggtgt
     4321 gccgcatacc ggaagatccc tgcgcatcta cgagaaatgt acgtgaatgc ataccaaagc
     4381 tttttgtgga atgaatgtgt caaagaaatc ctaaaaaata ctattgagaa aaagaagttg
     4441 tactcggtgg aatacgcgat cggatcactt ttgttcttta ccactctctc agagcaagaa
     4501 catggaaggc tcccgcagac gattcagacc atcagtgatc agatgacaat tactgatgat
     4561 gaacgaaagg ttattgatca tgtgcttgcg aaagaaggac tgagattgac tgattttagg
     4621 atcgaggatg aaacaggaaa tttctttaaa acacgagcac ggcaagttct gctaaaagct
     4681 gaagatttaa cgattggtac gtcggagaga gacgaactga acagcaaagg gaacgtagaa
     4741 cgatataaaa ttcaaatatc attttcatta tcgaaaggaa gttatgccac gatcatgacc
     4801 aaacgattat ttgggcatta aactc
//
LOCUS       DATMXJ010000008         5151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_57617, whole genome shotgun sequence.
ACCESSION   DATMXJ010000008 DATMXJ010000000
VERSION     DATMXJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57617"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VN377_00230"
     CDS             complement(<1..1404)
                     /locus_tag="VN377_00230"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWR26842.1"
                     /translation="MKLVICEKNIAARRIAYILSGGQVKSTHHGKIPVYSFTKNNEPW
                     DVIGLKGHIINLDYPSGFNQWTKIPPHELIEVEPCKNVSEKEIASALKTLVKDTPSLI
                     VATDFDREGELIGVEVVDLLRSYNPALTNIKRARYSAITPYEINTAFDNLTEVDYNLS
                     YAGEARQVIDLVWGAVLTRFISLTSRRLGRDFLSIGRVQSPTLALLVEREKEIRSFIP
                     KPYWVLLAQLKKDKPVDTTHEHGVFREEQPAQELYDKIKDSKEALVKEVKKIVTNELP
                     PAPFNTTTFIQAASYLGFSAAKAMSLAEELYMMGLTSYPRTDNTVYPPSLNINSILQK
                     LSQAHFSKEIKEIQTNGRNYPTRGKQQTTDHPPIHPVDAPSKKLGADMEKIYELICRR
                     FFATLAKDAISETTDVWFDISGEKFVTSGYRLVEANWRHLYPYFKEKKKQLPELIEGE
                     KLEIMKITIKKDSTKPPQ"
     gene            1518..1964
                     /gene="purE"
                     /locus_tag="VN377_00235"
     CDS             1518..1964
                     /gene="purE"
                     /locus_tag="VN377_00235"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWR26843.1"
                     /translation="MKIQIIMGSSSDTPVAEKAKAILEDFGVSYDIKIASAHRTPDLL
                     KKIVEKSDADVFIGIAGLSAALPGTIAAHTIKPVIGVPVSGKVNLDAILSIIQMPPGI
                     PIGAVGLDRGENAALLAVEILAVKDTKLEKKLLEYRKKMREKALSR"
     gene            1976..2923
                     /gene="guaA"
                     /locus_tag="VN377_00240"
     CDS             1976..2923
                     /gene="guaA"
                     /locus_tag="VN377_00240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWR26844.1"
                     /translation="MFHSEKFIEKTISTLQKDIKGKALIAFSGGVDSTVCAALVNKAI
                     GKQLIAVHVDTGYMRKNESAQVKQMMEDLGLNYRYITASKEYCQALKGVEDPEKKRKI
                     IGEKFIRIFEQVGRKEKIDYLVQGTIAPDWIESGGGLRDTIKSHHNVGGLPKDMKLKL
                     VEPLRDLYKDEVRKVARALNLQVSERQPFPGPGLAIRIIGEASPERTTLVREACDIVE
                     KEIERAAEQGLMELPWQYFAVLIPIKTVGVHGDKRAYGNTIAIRAVQSVDAMTCHYST
                     IPHEVLDTISTKITNTLKEKVNRIVYDVTNKPPGTVEWE"
     gene            2929..3504
                     /locus_tag="VN377_00245"
     CDS             2929..3504
                     /locus_tag="VN377_00245"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase subunit A"
                     /protein_id="HWR26845.1"
                     /translation="MVRIYVIDNGGQWTHREWRTLRDLEVETQIVPNTTLMKELQKEN
                     IHGLVLSGGAPRIGLDGTLGNCAEYLDNADFPILGICAGHQFMARHFGGKVKPSKAPE
                     FGQIELTLCVEDDPLFIGVPKKSIIWESHNDEVTVLPKDFTKLGESENCKIQAMRFNK
                     KQFYGLQFHPEVEHTQFGEQIFKNFIQLCEE"
     gene            complement(3509..4012)
                     /locus_tag="VN377_00250"
     CDS             complement(3509..4012)
                     /locus_tag="VN377_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26846.1"
                     /translation="MEITACPVCGSKNIGIGTLGDGIISGLSSWNEVCRNCGYQGASL
                     IFESESQYNKFLEALTFQKKQDETQKTETKEKSEQGTDEKTTIRSGKKNYLFEFIVSV
                     VLTIVFFIILSGSNYFGMYSNLFSQSNIGNIVVYFLGSFVGVILFFFLFIVLGETLYR
                     SIRSKKK"
     gene            complement(4028..4579)
                     /locus_tag="VN377_00255"
     CDS             complement(4028..4579)
                     /locus_tag="VN377_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWR26847.1"
                     /translation="MRYFVSIGGASGSIYGIRLVQELNKQGHEIHLVVSEGAKKILQH
                     ETTYTYTILKTQAHAVYDNDDLCAGPASGSFPLDGMIIIPCSMKTLSAIANGFGDTLT
                     SRAASCCLKEGRKVILVLRETPLDLPGITNMLAAKQSGAVILPAMPAFYQKPERIEDL
                     VDFIVGKVFDQLGLQHSLFKRWK"
     gene            complement(4583..>5151)
                     /locus_tag="VN377_00260"
     CDS             complement(4583..>5151)
                     /locus_tag="VN377_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HWR26848.1"
                     /translation="VDETVQAIKKNSDLPVILFPSGAKFLSKYADAVFFMSLLNSNNL
                     GYVIREHVKGAPFVKYSGLEPIPMGYVIIEPGMTAGRIGEADLIKRNDIQAAVGYALA
                     AEYLGMKFFYIEAGSGAPEPVPNEMIQAVKKNTSIPLFVGGGIRDPETAKEKAHAGAN
                     VIITGTAIEKDAHFKTTLAHIIQALEGL"
BASE COUNT         1397 a         1076 c         1219 g         1459 t
ORIGIN      
        1 ctggggtggt tttgtagaat cttttttaat cgttattttc atgatctcga gtttttctcc
       61 ttcaatgagt tctggaagct gtttcttttt ctctttaaaa tacggataga ggtgcctcca
      121 gtttgcttcg acaagtctgt agccgctcgt gacgaatttc tccccggaga tatcgaacca
      181 gacgtcggtt gtctcagaga ttgcatcttt ggcaagggtg gcaaaaaatc gcctgcagat
      241 aagttcatag attttttcca tgtcagcgcc gagttttttc gatggcgcat caacagggtg
      301 aatcgggggg tgatccgtgg tttgttgttt tccacgtgtt gggtagtttc ttccatttgt
      361 ttgaatttct ttaatctctt ttgagaaatg agcctgtgat aatttttgta aaatactgtt
      421 gatgttcaac gagggtggat agaccgtgtt atctgttcga gggtacgatg tcaaccccat
      481 catgtacagt tcctcagcta ggctcattgc ttttgccgcg gaaaaaccta gatacgaggc
      541 tgcctggata aacgtcgtgg tgttgaaagg agcgggtgga agctcgttgg tgacaatttt
      601 ttttacttct ttgacaagtg cttctttact gtccttgatt ttatcataca gttcctgagc
      661 gggttgttct tccctgaaca ccccgtgctc gtgggtagta tcgacgggtt tgtctttctt
      721 cagttgcgct agaagcaccc aatatggttt tgggatgaag gatctgattt ctttttcccg
      781 ttcaactagc aaggctagcg tcggtgattg cactcgaccg atggaaagaa aatctcgtcc
      841 aagacgacgg gacgttaacg agataaatcg tgttaagacc gcgccccata ctaagtcgat
      901 gacttggcgt gcttcaccag cataagaaag attatagtcc acctcagtga gattgtcaaa
      961 agcggtattg atttcatagg gggtgatcgc gctatatctt gctcgtttta tattggtaag
     1021 agcaggatta tagcttcgca gaaggtcgac aacctccaca ccaatgagtt caccttctct
     1081 atcaaaatca gtcgcaacaa tcaatgaagg agtatctttc accaaggttt ttaatgcgga
     1141 ggcgatctct ttttcactga catttttaca aggctccacc tcgataagct catgaggggg
     1201 gatctttgtc cattgattaa aaccactggg gtaatcaagg ttgataatat ggcctttcaa
     1261 cccgatcacg tcccatggct cattgttttt tgtaaacgaa tacacaggga ttttcccatg
     1321 atgcgtactt tttacttgac caccggaaag aatgtacgcg attcttctcg cagcaatatt
     1381 tttctcacaa atcactaatt tcatggtcgt tcctagttcg agaactgaac attaagatgt
     1441 gatataatta gtttacgata gggaaaagag gaggttgaaa ctatttatat ggtgacggta
     1501 tttcctgatg tgagactatg aaaatacaga tcatcatggg gtcatcttcc gatacgcctg
     1561 ttgcagaaaa agcaaaggct atcctcgagg attttggggt ctcttatgat ataaagattg
     1621 cttctgcgca tcgtactccg gatttgctga aaaaaatcgt tgaaaaaagc gatgctgacg
     1681 ttttcatagg aatcgctggt ctgtcagctg cgttacctgg gaccattgct gcccacacga
     1741 tcaaaccagt tatcggggtt cccgtgagcg ggaaagtcaa tctcgatgct attctgtcca
     1801 tcattcaaat gccgccaggg atccctattg gagcggttgg tctggatcga ggggagaacg
     1861 cggcattgct tgcagtagaa atccttgctg tgaaagatac gaaattggaa aaaaaattac
     1921 ttgagtatcg gaaaaaaatg cgggaaaaag cactgtccag atagaggctt atcagatgtt
     1981 tcattcagag aaatttatag aaaaaacaat ttcaactctt cagaaagata tcaaggggaa
     2041 agcactgatt gcattctctg gtggggtgga tagtactgtc tgtgccgcat tggtcaacaa
     2101 agcgattgga aaacagttaa tcgctgtgca tgtggacacc gggtacatgc ggaagaacga
     2161 atccgcacaa gtcaaacaaa tgatggaaga ccttggatta aattatcggt acattacagc
     2221 aagtaaggag tattgtcaag cgttaaaagg agttgaagat ccggagaaaa aacgtaagat
     2281 catcggggaa aaattcatca gaattttcga acaagttgga cgaaaggaaa aaatcgatta
     2341 cctcgtgcag ggaacaatcg cacctgattg gatcgaatca ggtggtggtc tgcgtgatac
     2401 gattaagtca catcataatg ttggtggatt gcccaaggat atgaaattga aactcgttga
     2461 accgcttcgc gatctctata aggatgaggt gcggaaagtc gctcgtgcat tgaacctaca
     2521 ggtgtcagaa cggcaaccat tccccggccc ggggcttgcc attcgtatca ttggtgaggc
     2581 tagcccagaa cgcactacac ttgtgagaga agcttgcgat atcgttgaga aggaaatcga
     2641 acgagcggca gaacaagggc tcatggaact accctggcag tatttcgcgg tgttgattcc
     2701 cataaaaacc gtaggagtac atggcgacaa acgagcctat gggaacacga ttgcgatccg
     2761 tgccgtgcag agcgtcgatg cgatgacctg ccattatagc acgattccac atgaggtgct
     2821 tgatacaatc tcaacaaaaa tcaccaatac ccttaaggag aaagtgaatc gcatcgtcta
     2881 tgatgtgacc aacaaacctc ctggtacggt cgagtgggaa taaccagtat ggtacgcatc
     2941 tacgtgattg ataacggtgg acaatggacg catcgtgaat ggcgcacgct ccgtgacctc
     3001 gaggttgaga cacaaatcgt accgaacacg acactgatga aggaattaca aaaagaaaac
     3061 atacatggtc ttgttctctc aggcggagca ccacggatag gactggatgg cacgttaggg
     3121 aattgcgcgg aatatctcga taacgctgat tttccgattc tagggatttg tgcaggtcat
     3181 caattcatgg cacgtcattt tggtgggaag gttaaacctt cgaaggcacc tgagtttgga
     3241 cagattgaac ttaccctctg cgtcgaggat gaccctctct ttatcggggt gccgaaaaaa
     3301 tcgatcatct gggaatccca caacgatgag gtgactgtcc tgccaaagga ttttacgaaa
     3361 cttggcgaaa gtgaaaattg taaaattcag gcgatgcggt ttaataaaaa acagttctat
     3421 ggcctgcagt ttcaccctga ggtggaacat acacaatttg gggaacagat ctttaagaat
     3481 ttcattcaac tgtgtgaaga gtaacgaatt attttttctt actacggatg cttcgataga
     3541 gtgtttctcc taacacgatg aaaaggaaga aaaagagtat gacacctacg aaactcccaa
     3601 ggaaatatac tacaatatta ccgatattgc tttgggagaa aaggttggaa tacatgccaa
     3661 aataattact tccagacagg atgatgaaaa acacgatggt gagaacaaca gatacgataa
     3721 attcaaagag atagttcttt tttcctgaac gaatggttgt tttttcatct gtgccttgtt
     3781 cagatttttc ttttgtttct gtcttttgtg tttcatcctg ttttttttga aacgtgagag
     3841 cttctaagaa tttattatat tgtgattcag attcaaagat aagagatgcc ccttgatagc
     3901 cgcagttcct acatacctcg ttccatgagg ataacccgga gatgatcccg tcaccgaggg
     3961 ttccgattcc gatattctta cttccacaaa ccggacatgc tgtaatttcc attggtcaca
     4021 ccattgatta tttccatcgt ttgaacaacg agtgttgaag gccgagctga tcgaagactt
     4081 tcccaacgat gaaatcaaca aggtcctcga ttcgttctgg tttttgatag aacgcaggca
     4141 tggcgggcag gataactgcc ccgctttgtt ttgccgcaag catgtttgta atccctggga
     4201 ggtccaaggg cgtctcacga aggaccagta ttacctttcg tccttccttc agacaacaac
     4261 ttgccgcccg ggacgttaat gtatctccaa aaccattcgc aattgcagaa agtgttttca
     4321 tactacaggg gataataatc ataccatcaa gcgggaatga accgctcgct ggaccggcac
     4381 ataaatcatc attgtcgtac acggcatgtg cttgtgtctt gaggatcgta tatgtatatg
     4441 tcgtttcatg ttgaaggatt ttctttgccc cttcagaaac gaccaggtga atttcgtgac
     4501 cctgcttgtt taattcttgg actaacctga tgccatagat actaccggag gctcctccta
     4561 tgcttacaaa atatctcata gcttacaaac cttctagtgc ttgaataata tgagcgagcg
     4621 ttgttttgaa atgggcatct ttttcaatag cggtcccggt aataatgacg tttgccccgg
     4681 catgtgcttt ttcctttgcg gtttctgggt ctcggatgcc cccaccgaca aagagcggga
     4741 tagatgtgtt tttcttcacc gcctgaatca tctcattggg gacaggttca ggagctccag
     4801 agccagcctc aatgtagaaa aacttcatcc caaggtattc cgctgccagg gcatatccga
     4861 ccgcggcttg aatatcattt cttttaataa gatcagcttc tccgatacga cccgctgtca
     4921 tccctggttc tatgatgacg taccccatcg gaatcggctc aagcccggag tatttgacaa
     4981 atggtgctcc tttgacatgt tcccggatca catatcctag attatttgag ttcagaaggc
     5041 tcatgaagaa taccgcgtca gcgtatttgc tgaggaattt cgcgccagat gggaagagga
     5101 tgacggggag atctgaattt tttttgatgg cctggaccgt ctcgtctacc t
//
LOCUS       DATMXJ010000009         5626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_41947, whole genome shotgun sequence.
ACCESSION   DATMXJ010000009 DATMXJ010000000
VERSION     DATMXJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41947"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VN377_00265"
     CDS             <1..344
                     /locus_tag="VN377_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26849.1"
                     /translation="MYAYQACSVFIVSAVIPPETNVITGLSTLWNIMGVPDDVSVVKQ
                     NLVVRYDGVDYNWTQATTSNNPTGSPLVLGFIYGWSRSGQSYLLSDSLDPGYAYWMYA
                     YHTCQLTKGGT"
     gene            347..>5626
                     /locus_tag="VN377_00270"
     CDS             347..>5626
                     /locus_tag="VN377_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR26850.1"
                     /translation="MNHKMSIYKKAVGFAIVALFIAGAFVPANGFILRDTTVSTKPSP
                     PTLQKDINPILILDTFEQTQNNIDISDGLEQKQKGPPQPLNPLDPWWNPSFSYRKEIT
                     IDHTKITANLTNFPVLISLSSDVDLAAHTQSDGDDIVFINNATGTKLNHEIEMYNSTG
                     RLVAWINVTNLSKTADTKLYMYYGNSSCGSQQNPKGVWDSNYVSVWHLNEDPGVAGNG
                     GIKDSTRYYNNGTDMGTMNAADHVTGKIGYGIDFDGTDDVIRIDNSNVVGHCLDFTSG
                     PFTIEAWFTARTRTGTIICKRDGVPSNADQYQMYLDPVTNGIYFRAGLEYGYGSDTLS
                     TNTWYYGAAVVDSNEWPNITRDGVLRTWSNTPGAGSPPYVFTHRNVNVSIGARWNTYP
                     ATGYLLPGIIDEIRISKINRSSSWLSTSYNNQNNPIGFSTLGSEETLGGNQAPVITSS
                     TPVDGTSFVKLNPTLAITINDPDGDTMNISFKTNATGSWSVIGSNLSIGNGTYQQTPT
                     TMNSYHTKYYWSVNVNDGPKWTNKTYWFKTEPEPGIWWDTNWMYRKRIEISHTQIASN
                     LTNFPVLITFPSDADLATDAQDDGDDLVFTDFNGNQLHHENELFDGVTGKLIIWVNVT
                     SLSHLNDTVVYLYYGNALCDNQQNINGVWKSDYVAVWHLNQDPGVVGTSGILDSTNYS
                     NDGTDMGSMDAADHVIGKIGYGVDFDGTDDVIKIENSWTAGHILDFTNESSFTLEAWF
                     NCRTTTNQGTLINKRDSPTASIHYDQYQFHLQPNPQFRANQEFGGGTDTLGTSTWYYG
                     AAVVTSNGTIGGWPIIYRDGILKTWADNTGTRPYSIIHRNVNVSIGARWAGYPTAGYL
                     FSGVIDEVRISKTNRNSSWLLTCYNNQNNPGGFYIVTPKETGTRPEVPQVTDEVPNNS
                     TTGTILNPLLTVNVIDYQGNTMNVWFKTNASGTWTVIGSNTSVGNGTYRQIPTAMISY
                     NTKYYWSVNVFDGTYWMNKTFWFMTKINYPPVMTNLNPTNGSIGNLISLAQLSITLND
                     PEGNPITYSITTSPNIGSKSETGINNGTITVDVSGLAYNTTYSWSVKATDSAGSGQWT
                     NNTYWFKTERGPGTWWNTNWAYRKEITIDHTKVATNLTNFPVLIKLSSDADLATDAQD
                     DGDDLVFTEYNTGILNHEIELFNGTTGQLVAWVNSSSLSRTADTILYLYYGNGVVGNQ
                     QNPEGVWDIHYKGVWHFNNSILDSTTNNDDGTNYGSTNAIGTIGGSRYFDGNDYITAP
                     QHCIGANQNWTASCWFRSNSTSSMVYHYFFSTGPYTGPTSYGAFQLYHNMNDATYLPI
                     DEVRTRLQDQDSDFSQLYQYGGSAAEDTWHLVHVTWNALTHNFRIYVNGSLVNQSTNA
                     AVTGAGTTLPLYIGARGDLDTQRYHIGSIDEARIISQTKTAGWITTEYNNQYNPSGFS
                     TLGDEEEAPLVNQPPIFSSENPTDNTGILDINIPSVSVLIQDPEGDAFDWTIEGQYVT
                     SAGANGAVNGTKSASLITPLPYITNIIWYVNATDGFEWTREVYNFTTRAQYIPGIPSG
                     FTATAMNRTRIDLSWTKGDKTDKTCIERNLVASWNRGEGTLIYNNTGTNYQDTGLSQN
                     IHYYYQALSWNTTDHVYSTTSAAADATTFANQLPVFSGENPADNTGNIGINTPSVNVL
                     ILDPDGDVFDWMIQGQYVTNAGANDASNGTKSASLITPLPFSTNVIWYVNATDGVGWA
                     RMVYNFTTRSQYIPSIPSEFTATAMSRTRIDLAW"
BASE COUNT         1710 a         1169 c         1266 g         1481 t
ORIGIN      
        1 ggatgtacgc gtatcaggct tgttccgtgt ttatagtgtc cgcggtgatt ccaccagaga
       61 caaatgtgat cactgggttg tcaacgctgt ggaacattat gggtgtccca gatgatgttt
      121 ccgtcgtaaa acagaatctc gtggtgaggt atgatggtgt ggattataat tggacgcagg
      181 cgaccacgag caataatccc acaggcagtc cgttggttct tgggtttatc tatggttgga
      241 gtcggtctgg tcaaagttat ctgttgtctg attctcttga tccgggttat gcgtactgga
      301 tgtacgcgta tcatacttgt caattaacga aagggggaac ttagaaatga accataaaat
      361 gagtatctat aagaaggctg ttggtttcgc gattgtagcg ctcttcatcg ctggtgcgtt
      421 tgtgcctgca aatggattca tccttaggga cacaacggtg tcgaccaaac catctccacc
      481 gacattgcaa aaagacatca atcctatcct catcctagat acatttgaac aaactcaaaa
      541 taatatcgat atctctgatg gtttagaaca aaagcaaaaa gggccgccac aaccacttaa
      601 tccattagat ccctggtgga atccttcatt cagttatcgg aaggagatta ccattgatca
      661 tacgaagatt actgcgaatc tgacgaattt cccggttttg atttctctat cctccgatgt
      721 agatcttgca gctcatactc aatctgatgg cgatgatatt gtttttataa ataatgcaac
      781 gggaacaaag ttgaaccatg agattgaaat gtataacagt accggtcgtt tagtcgcctg
      841 gataaatgtt acgaacctct caaagactgc ggatacaaaa ttgtatatgt attatggaaa
      901 ctcgtcgtgt gggagtcagc agaacccaaa aggggtatgg gactctaact atgtatcggt
      961 ctggcatctc aatgaagatc caggtgttgc tgggaatggt ggtatcaaag attcaacacg
     1021 atattacaac aatgggacag atatgggaac tatgaatgct gcggatcatg tgacaggaaa
     1081 gatcggatat gggatcgatt tcgatggaac tgatgatgtg atccggattg ataactccaa
     1141 tgttgttggc cattgccttg attttacgag tggcccgttc accattgaag catggtttac
     1201 tgcgagaacc agaactggta caatcatatg taaaagagat ggtgtcccta gtaatgctga
     1261 tcaataccaa atgtatctag accccgttac taacggaatt tatttcagag ctggacttga
     1321 atatggttat ggatctgaca cattatcaac gaacacttgg tattatggtg cagctgtcgt
     1381 ggactccaat gaatggccaa acatcacacg agatggtgta ttacgaacat ggtcaaatac
     1441 accaggagca gggtcaccac cctatgtgtt tacacatcga aatgtgaatg tttctattgg
     1501 tgcacgatgg aatacgtatc ctgccacagg gtatctttta cctggaatca ttgatgaaat
     1561 acgtatctcc aaaattaatc ggagtagtag ctggttatct acctcttata acaatcagaa
     1621 taatcctatt gggttctcca ctcttggatc tgaagaaaca cttggaggga atcaagcacc
     1681 agtaattaca agctcaactc ctgtggatgg tacaagtttt gtgaagttaa acccaactct
     1741 cgcgattacg ataaacgatc ctgatggaga tacgatgaat atctcattta aaacaaatgc
     1801 tactgggagc tggtccgtta tcggaagtaa cctcagtatt ggcaatggaa catatcaaca
     1861 gaccccaaca acaatgaatt cctatcatac caaatactac tggagtgtga atgtgaatga
     1921 tggaccaaag tggacgaaca agacgtactg gttcaagaca gaaccagaac ccggtatttg
     1981 gtgggacaca aactggatgt atcgaaaaag aatcgaaatt agtcatacgc agatcgcctc
     2041 gaacctaacg aatttccctg ttttaattac ctttccctcc gatgccgatc ttgctacaga
     2101 tgcacaagat gatggtgatg atctggtctt cactgacttt aacggaaatc aactccatca
     2161 tgaaaatgaa ctatttgacg gggtaacagg gaaactcatc atttgggtga atgtcacaag
     2221 tctatcgcat ctcaatgata cggtcgtgta tttatactat ggaaatgcct tgtgtgacaa
     2281 tcaacagaat ataaatggcg tctggaaatc agactatgtt gcagtctggc atctcaacca
     2341 agaccccgga gtcgttggga caagtggcat cctagattcg acaaattatt caaacgatgg
     2401 gactgacatg gggagtatgg atgctgcgga tcacgtgatt gggaaaatcg gttacggggt
     2461 tgatttcgac ggaactgatg atgtcataaa gattgagaac tcctggacag ctggtcatat
     2521 ccttgatttt acgaatgaat cctcctttac tcttgaagca tggtttaact gtcggacaac
     2581 tacgaaccaa ggaacactca ttaacaaacg agatagtcct actgccagta ttcattatga
     2641 tcaatatcaa ttccatttac aaccaaatcc gcaattcagg gcgaaccaag aatttggggg
     2701 aggaacagac actcttggaa catcaacatg gtattatggt gcggcggtag tgacgagtaa
     2761 tggtacgatt ggtggctggc caatcatcta ccgtgatggt attttgaaaa cctgggcgga
     2821 taacacggga acacgaccgt atagtattat ccatagaaat gtcaatgttt caatcggtgc
     2881 acgatgggca ggatatccca ccgctgggta tctctttagt ggagttatcg atgaagtacg
     2941 tatttctaaa actaatcgaa atagcagctg gttgctcacc tgctataata accagaacaa
     3001 ccctggtgga ttctacatag ttactcccaa agagacagga acaagacccg aggtgcctca
     3061 ggtaactgat gaagtaccaa ataatagtac aaccggtaca attttaaatc ctctactcac
     3121 tgtgaacgtc attgattatc aagggaacac aatgaatgtc tggtttaaaa ccaacgcatc
     3181 aggaacctgg actgttatcg ggagtaacac gagcgttgga aatggtacct atcgtcaaat
     3241 cccaacggcg atgatttcct ataacacaaa atactattgg agtgtgaacg tttttgatgg
     3301 gacgtattgg atgaataaaa cgttttggtt tatgacaaag ataaattacc cacctgtcat
     3361 gacaaacctg aatccaacga atggatccat aggtaatttg atctcgcttg cacaactgag
     3421 cataacgtta aatgatcctg agggaaatcc aattacgtac tcgataacca catctccaaa
     3481 tattggaagt aaaagcgaga cgggaatcaa taatggaact atcactgtcg atgtaagtgg
     3541 acttgcatat aacacaactt actcttggtc tgtgaaggcc actgactctg ccgggagtgg
     3601 acagtggaca aacaacacct actggtttaa aacagaacga ggaccgggaa cctggtggaa
     3661 taccaattgg gcgtatcgta aggaaataac gattgaccat acaaaggttg ctacaaacct
     3721 tacaaacttc ccagttttaa ttaaactctc ttcagatgct gatcttgcta cagatgcaca
     3781 agatgatggt gatgatcttg tatttacgga atataatacg gggatactta atcatgagat
     3841 cgaacttttt aacggaacca ctggtcaact tgttgcgtgg gtgaacagtt caagtctctc
     3901 ccgaactgca gacaccattt tatatctcta ttatgggaat ggcgttgtcg gtaatcagca
     3961 aaaccccgaa ggagtatggg atatacacta taaaggagtc tggcatttca ataacagtat
     4021 ccttgatagc actacaaata acgacgatgg gacaaactat ggatcaacaa atgctatagg
     4081 aacaatcgga ggaagtcgtt atttcgacgg aaacgactac atcactgccc cgcagcattg
     4141 tattggtgca aaccagaact ggaccgcaag ctgctggttc cgatccaact ccacctcaag
     4201 tatggtgtac cattacttct tctcgactgg cccatacaca ggcccaacct cttatggagc
     4261 gtttcaactc taccataata tgaatgatgc gacttatcta ccaattgatg aagtgcgtac
     4321 tcggctacaa gatcaagatt ctgatttttc acagctttat caatatggtg gcagtgctgc
     4381 tgaggacaca tggcatcttg ttcacgtgac ctggaatgca ttaacacaca actttagaat
     4441 ctacgtaaac ggttctttag taaaccaatc tacgaatgcc gctgttactg gtgctggtac
     4501 taccctcccc ttatacatag gtgcgcgagg tgacctcgat acccaacgat accatattgg
     4561 gagtatagat gaggctcgca tcatcagtca aacaaaaaca gcaggatgga ttactactga
     4621 atacaacaac caatacaatc cgagtggatt ctccacatta ggagatgagg aagaagcacc
     4681 actggtgaac caacctccta tattctcaag tgagaatccg acggataaca cagggatcct
     4741 tgatatcaac ataccaagtg tgagtgtttt gatccaagat cctgagggtg atgcgtttga
     4801 ttggacgatt gaaggtcaat atgtgactag cgcgggtgca aatggtgcag taaatggaac
     4861 aaaatctgca agtctgatca ccccgttacc gtatattacg aatattatct ggtatgtaaa
     4921 cgcaactgat ggatttgaat ggactcgtga agtgtacaac tttaccacac gtgcacaata
     4981 tatcccaggc atcccaagtg gatttacagc gactgcaatg aacagaaccc gtatcgacct
     5041 ctcgtggacg aagggagata agactgataa gacgtgcatt gaacgaaacc tggtcgcatc
     5101 ttggaataga ggcgaaggga cattaatcta caataacacc gggacaaact atcaggatac
     5161 aggattatct cagaatatac attactatta tcaagcatta agctggaaca ctactgatca
     5221 tgtgtatagt actacctctg ctgcagctga tgctaccaca tttgcgaatc agcttcctgt
     5281 gttcagcggg gagaatcctg cggataacac agggaacatc ggtatcaaca cccccagtgt
     5341 aaatgtcctg atcctggatc ctgatggtga tgtgtttgat tggatgattc aagggcaata
     5401 tgtgaccaat gccggtgcca acgatgcgag caatgggaca aaatcagcga gcctcatcac
     5461 cccgttgcca tttagcacga atgttatctg gtatgtgaac gcgactgatg gggttggatg
     5521 ggctcgcatg gtctataact tcacgacacg gtctcagtat atccctagta tcccaagtga
     5581 attcactgca actgcgatga gcagaacccg tatcgatctc gcatgg
//
LOCUS       DATMXJ010000010         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36736, whole genome shotgun sequence.
ACCESSION   DATMXJ010000010 DATMXJ010000000
VERSION     DATMXJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36736"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1272..3776)
                     /locus_tag="VN377_00275"
     CDS             complement(1272..3776)
                     /locus_tag="VN377_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR26851.1"
                     /translation="MKKIIGIFVCMLMISGALASATNLLEDKKASNVDAGTITITIPV
                     GSYQVIKEKQGYEITIDNFGRLLSPGKPNLPSKIFSIAIPPGAEVQDVTFESKDGILL
                     SGEYKIAPNVLSRVIGPEDSGMTARDLQQYEQNYKSVYGQDNPYPQSIGEVVGTGGYR
                     KYNLVDVRITPIIYYPFAGKLLYHPDITVTVQYTIPQGFSSEDIMIDNLPRAEQTAKE
                     IILNYDQAKNWYTEKPVNREQYDYVIITTDALTSSVTSLADWESAKGHSVNVVTTEWI
                     ASNYAGYDLSAKMRAFLLDKYASEDWGILDVCLIGDVDDVPMRTTWQEPETDYYYAEL
                     SLPDSQSWDIDGDHQYGENSDPIDFETEVNVGRIPWSDPDTVQSICEKSVAYEQNTDD
                     SFKKNILLLGAYFWSDTDNAVLMEAKVDQEWMTDWTMTRMYEDAQSSYPCDYDLTYNN
                     VLSVWSSGSYAFVDWAGHGSPDAAYEYYPSQPFVDETTCLSLNDEFPSIVFADSCSNS
                     DTDYLNIGQAMLKQGAIGFLGSNKIAYGQPGWNDPMDGSSQSLDYFFTTYCTSGNYTQ
                     GQAHQWSLRQMYVNNLWTAGTYMEMFEWGSLFGNPDLTMGTVTTSDPPTVPLKPIGPT
                     HGTIDRQYTFSSVSTDPNNDQIYYLFNWGDETSSAWLGPYTSGLTISAAHTWSAIGEY
                     NVKVKAKDTNGAISDWSEPQTITIVLNDAPDTPTINGPATGVPGKAYLFRMQTTDANG
                     DNVYYYVDWGDNTTTEWIGPFNSGVEATTTHTWSEQGTYTVKVKAKDVVGDESDWGTL
                     EIPIPFEYRFSFQTYLQHLFEMFPHMFPILRHLMGY"
BASE COUNT         1076 a          768 c          770 g         1204 t
ORIGIN      
        1 atgtaatatt gtgtaaattc taatacaaaa attttctaca gaattattta acgaaatagt
       61 atctatgcgg cagagatttc gttgaatgta ctgtcgtgta cgttggaatt aaggtgatgc
      121 atcgggattt gtaaatttac attcaccaat attttctcct ttatacagaa gccgaattat
      181 ccatttgtct tgggtcctaa actttcctag attgttaaaa gttatctgat ttgaaatccc
      241 aaacacagtt gggttagtat ccctgttcgt aagagtagct ccaatcatag aagtctaatt
      301 tatgtcactc gtcgcgttta ttgctgcaat ggaacaaaat gctggattca atggtccatc
      361 aataattcct ataatagtta cgcatgattc agattgtgtc atacgtactt ctggagtgcc
      421 atatggacct tgtggatttt taaagacact ttgaccaatg atgtcccatt ataaagcatt
      481 agtatgcccc atattattcc gagtggaaaa tctgatagat tatgaaaagt aattatatct
      541 ccaataccta aaacgaacag gtcaccgtta ccctcggtta tgatcgacct aagagcagat
      601 gtttgatttt ctttactatt attgtcaatt gcgatgacgt atatttgaga ttggttgata
      661 ttagcatttt tcatatccat aatttcaagg taaaattcaa attgagccct agttatgtta
      721 ggttttttat ttgattcatt aacgataatt gatgttgttg cggtaaatgt tgttgtggag
      781 gattttgtcg taagagtaaa tattgttgtc tctgttgatg tgatattacg aatacctgtg
      841 agattcacag taacgattcc attgtcaatg gttacagagg tggcatctaa aacagttcaa
      901 cgtattttag cagtgttcct aattggattt caattggcat tgtctcaaat gaagaaataa
      961 caggtttttt ttgttcgttg caaccattta aattattgca tagaaaacga ggatggctcc
     1021 tatgatgact atttgttttt tcatttctat ggaacctcca gaatttattc tcataacatc
     1081 ttctcctata cgaatataaa gaagcgagtt aaatgaaagt attttatttt aataggaaac
     1141 tactatgaga ctaaatatga agcaacctat catctagagg tttttctttt gcattgtgaa
     1201 tcgatagatc aattgatagc gtttgatgtt tgtagagtac aaataaaaaa aataaagaaa
     1261 aagagaggtg tctaataccc cataagatgt cgcaaaatcg ggaacatatg ggggaacatc
     1321 tcgaaaagat gctgaaggta tgtctggaat gagaaccgat actcgaacgg tatcgggatt
     1381 tccaatgtgc cccagtcact ttcatcacca acaacatctt ttgctttgac cttgacggtg
     1441 tatgttcctt gttcgctcca agtatgcgtt gtagttgctt cgaccccgga attgaaggga
     1501 ccgatccact cagtcgtagt gttatctccc cagtcaacat agtagtatac attatctcca
     1561 tttgcatctg ttgtctgcat cctaaacaga tacgctttcc ccgggacccc cgtcgcaggt
     1621 ccattgattg ttggagtgtc tggtgcatca ttgaggacaa tggttatcgt ttgaggctct
     1681 gaccagtcac ttatcgctcc gttagtatct ttggctttca cttttacatt atactctcca
     1741 atggccgacc aagtatgagc tgcagaaatc gtcaatcccg aagtatatgg cccaagccat
     1801 gcactcgatg tctcatcacc ccaattgaac agatagtaga tttgatcatt gttagggtcg
     1861 gtacttacac ttgaaaatgt gtactgtcta tctatagttc catgagtagg tccaattggt
     1921 ttcaatggta cggttggcgg gtcacttgtg gtaactgtgc ccatggtaag atcaggattg
     1981 ccgaaaagtg atccccattc gaacatttcc atatatgtac cagcagtcca caggttgttg
     2041 acatacatct gccgcaatga ccattgatga gcctgtcctt gggtataatt cccagaggta
     2101 cagtacgtgg taaagaagta atcaagtgac tgacttgatc catccattgg atcattccaa
     2161 ccaggttggc cataagcgat tttattggat ccaaggaatc caatagcacc ttgtttcagc
     2221 atcgcctgac cgatgttcag ataatcagta tccgagttgc tacaagaatc tgcgaagaca
     2281 attgatggaa attcgtcatt caaagaaaga cacgtggttt catcaacaaa aggctgcgat
     2341 ggataatact catatgctgc gtcaggtgac ccatgtcctg cccagtcgac gaacgcataa
     2401 ctacctgagg accaaaccga taagacatta ttatacgtca gatcgtaatc gcagggatat
     2461 gatgattggg catcctcgta catccgagtc atcgtccaat ccgtcatcca ttcctgatcg
     2521 actttcgctt ccatgagaac tgcgttatcg gtatctgacc agaaatacgc accaagcagg
     2581 agaatgtttt tcttaaatga atcatcagtg ttctgttcgt aggcgacaga cttttcacag
     2641 atactttgaa ccgtatcagg gtcgctccag gggatacgac ctacgtttac ttcagtctca
     2701 aaatcgattg gatcagaatt ttccccatat tgatgatctc catcaatatc ccatgattga
     2761 gaatccggca gggaaagctc agcgtagtag taatcggttt caggttcttg ccaagtggtt
     2821 cgcattggta cgtcatcgac atcaccaatg agacacacat ccagaatacc ccaatcttct
     2881 gaagcgtatt tatccaggag aaacgctctc atttttgctg aaaggtcgta acccgcgtaa
     2941 ttactcgcga tccattcagt cgtaaccacg ttgacgctat gtcctttagc actttcccag
     3001 tctgcaagtg atgtcaccga tgatgtcaat gcatcagtcg taatgatgac gtaatcatat
     3061 tgttccctgt tgactggttt ttctgtgtac caattcttcg cttgatcgta attaagaatt
     3121 atttcttttg cagtctgttc agctcttggc aggttatcaa tcatgatatc ctccgaggaa
     3181 aatccctgtg gaatcgtata ttggacggtc acagtaatat ctggatggta gagtaattta
     3241 cctgcgaacg ggtaataaat aattggggtg attctcacat cgacaaggtt atatttacga
     3301 tatccaccag ttccgacaac ctccccgatc gattgtgggt acgggttgtc ctgtccgtac
     3361 actgatttat agttctgctc atattgttgc agatcacgag cagtcatacc ggaatcctca
     3421 ggaccgatca cgcgtgacaa tacattcggt gcgatcttat attcaccgga tagaagaatg
     3481 ccatcttttg attcaaaagt aacatcttgg acttctgcgc ccggtggaat cgcaatagag
     3541 aagatcttcg aaggcaggtt tggttttccc gggctaagta aacgaccaaa attatcaatt
     3601 gttatttcat acccttgttt ttctttgata acctgatagg atcctacggg gatagttata
     3661 gtgattgtgc cggcatcgac attagaagct tttttgtctt ctagtaagtt ggtggcactg
     3721 gccagggcac cgcttatcat tagcatacat acaaaaatac ctattatttt cttcataata
     3781 aattactacc tcgtccgata taaatacaag agtaatac
//
LOCUS       DATMXJ010000011         9482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_62866, whole genome shotgun sequence.
ACCESSION   DATMXJ010000011 DATMXJ010000000
VERSION     DATMXJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9482
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62866"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>9482)
                     /locus_tag="VN377_00280"
     CDS             complement(<1..>9482)
                     /locus_tag="VN377_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HWR26852.1"
                     /translation="TALTGSLSVTTANWHIGDNGNNAMFFKGVVDEVQIANTCFSAGW
                     IKTFYNNLNTPSKFHLLSIEENQSGFPPVISHEKPVHTSVNNQFNPTLEVTVTDLNND
                     NVYCEFWTNKSGSWHKVRNTTIYNGFGIISDATENITTPNTKYWWSVNATDLYGSRKW
                     TNKTYWFVTGVGYPIISEVYPDDQDALYNPVLSVKIQDTSSNYLTVIFRKKIMDVWET
                     INTYHGYSGVYTQTTQNMDVKDQTYYWSINVSYGSSWINRSYSFTAYAFIQKWYSNTY
                     CRQTLGPLSADVNHDGIHEIFLTGDRKAICVNGLNGNLIWEYSHNLIMDHSPFELADL
                     NNDGIEEIVISCNRTGPFGATIALHADDGSVYWISEGPSDGKHPVIADVDGNGYPYVY
                     VASDAYGGSRLCKLRGTDGAIVKAVSIYYPCYGGLSAADIENDGNIEILLADYNGEPG
                     KGIHCYDKDLNLIWYQRARGDPQLPILVDVNNDGVLDSVTTAYSNGPVQVINGKTAQY
                     IVNSGGYPNHSPNSVYDIDGDGHLEILIASSSPCVVFDLTTKTVDATLITCKEAPYMA
                     NVIGDDKLEILATWWDKLTIYNSTYQIVDTIDLPLQRCTLVQDIDNDGQNELVFVSEN
                     HLVAYETTVSAPTPRVRSVNAGYSERRTGAAVYIPPPGAPQPILKEASPNGSLDVKLN
                     PTLSVHAIDFHYDRMNIIVCTNASGSWVTLASYTNVGNGWYNVTPTMMNKLDTTYYWR
                     VIAVDPLADNLITNKTYHFKTKSAPKIKSVNISSQTPFEGQDVNISARITEDVHMAET
                     KLRLNYPNGSYVNKSLYRYQWTKLTYDNFENNKWGNYTAGGANCGLYLGDWRLVHQGS
                     YVANIEANSGVASSFNLTHPIDIATPSYNAIKIDFWMNAYGMGDGGRYYLEYYDGTQW
                     QVRKIYEQNTGLFTPAIYTNTYYKFANWLFFHDTVWINKSTYNLPSNLNIRFRCASGG
                     TGSDVYLDQIYINATRLEPTLYTSIESYTLPGQYSYSIWCKDINGNTNTSATSQFTVL
                     VDTGLPQIANILVTPSTAIQGIPVNITCNVQDNVGVQDVRINISDPNANIVNYSIITN
                     KINPNTYYYNQKYFTVGTYHFFIWATDPYNNVAKSQTISFVITKSPPLLSNVTPSNQS
                     TNIHLNPTLSVSITDYSHDAVEWWISTNATGTWIPLAHSTLGNGNGVITAITTTMQDY
                     STRYYWRVNTREGTLWTNKTYSFTTEPINTSIASISPYAIISPTKTLTASGSPTLDNV
                     TLWYRYSPDNITWEGSSINWWNTAYHYRTKINFTEPQGIARTKTHVLITITLPLGRLS
                     NENDAALVDNGIQIPFDGYALTTQNGWVTTLQGLAEINMSAYQTKSCYLYYDPVNAAT
                     EKLPTTTGWSYIGATSYSGTNYVDLVPADTDCNGDSPTGEFTDVCGAVDYIKLNEWSY
                     FKAPQTGTLYFKTGSDDGSGLWLNDSLIVSDNNGGHGVQWRDSTPQSLNSGRYYALEI
                     DWAENDGGQELRNRWNTAPGDDSTGSIIDTEGYTFYGDEWQITTTLSSEEHNQGTSNK
                     FNTDTIAPWQWEFNFPNSTGYYQFFSLGRKTGIANEVEPQYADAICFYNYTGNRTPVL
                     QSPTPPDNSNNVPVGLSTLTVQMSDPENDFFNWTIHTSPNIGSASENHVNNGMKTCPI
                     STLNLGATYTWYVNATDLGSHRWTNKTYTFTVQDDAPTVTLNSPTNGATISSSQITLN
                     CTSTDDVNLHNVTLYFGSTDLANGNVNVRVSTGTDDAEEYLSTHAIDLVSTDLELIQD
                     PNQGNQEVGMRFQNVAIPPGATITNAYIEFEVDELNSDTTNLNIYGQAIDNAPTFTST
                     ASDITSRIKTSSFAAWNNVPSWDTESQKKHTPDASTVIQEIINRPGWSSGNSMVIIIT
                     GTGHRTAEAYEGESANAPLLVVNYTTGGIPVWHADQVKTISGITNTTTFIVNLNPGSY
                     KWNCRAYDAGGHNAFAPSNYTFTVLPPQYTLAMNTIGSGSVTKNPNQSTYNSGTIVTL
                     TATPASGWSFTGWSGDLTGSTNPTTITMTGNKAVTATFTINPTTAGWSAYNDLAGAAS
                     SAPATNYHSGNSGLLKDYATGTNTAVSVSISGGTNDGTGPGVLPSGTDAYKIFNGKIN
                     PDSILHYSGGNHIITFTGLDSSKRYRFVHWADRADSSNPYNSTVTITDVTSFTKNSST
                     AVTHSQTTLPYDTASYYTGYNSVHGYVAGFTNIDPGTDGDMTITVSGATKWYSGAFML
                     QELLPIQVNFTIIALPDTQNYASGYPTIFTNQTQWIVAHKNALNIVWVTNEGDITNGG
                     SDLEFQRADAAYDLLEDPATTQLPYGIPYTIIQGNHDHATNLFNTYFNYTRFAGRTYY
                     GGHYPTTKNDNNYALFSAGGMDFIAIGLDYAADTNELAWAENLTQTYSTRRAIVVSHS
                     ILNNAAGDWTGEGINIYNALKDNSNLFLMLCGHMHYEGRRADTYNGNVINTLLADYQD
                     YPNGGNGWLRIMTFCPATNEIKVKTYSPYLDQYQTDADSQFTLSYTMNPSPALYTLTV
                     NTVGSGLVAKTPDQSTYSLGTVVTLTATPAQGYSFTGWSGDLTGSINPTTITMNGNKT
                     VTATFTALPPSQGGILETFNTGFTTGQTVGAHADWYDGGSGPVITAGNGVGGSIGLAP
                     SGTIFTWEAHPFNWNDPDFIGVNLRMDFESSSTGKFDDDRIGWATSVTGTGSEIQFGV
                     QLDDTDGGIVTFWRNSADARVQQPITTYSGITSNTYYRLRANITKLTATAAKIDVSLT
                     KLDGSGNEIGVVASGSVANTSSYGSNAPATRYFTVTSMWPTFKSYSTEPGDADNAYYE
                     LIQPQSWLRIYNPYEDVNFNTANSYKGNFHTHTTYSDGSSTPDAVIDYYHDIGHYDIL
                     ALTDHSHNTWPWSTWITEEPVTPSNTSEYYPTLNMLAISANEPSNSHNIGSYLNDYAG
                     GDLDAAFQYIQNHHGLSIFMHPGRYSYAPSWYNTYFNTYDDTILGVEAYNQGDRYPGD
                     RVKWDSINKERDPDNLIWGFSDDDMHSLAGNAFRNYQHFFMNNLNESDFRNAMINGAF
                     FFSYEPDGTNQADPNYGHAKTPKLTNVIISGSVIQIAGTDYTSIQWYNETTAVVGTGT
                     SINVSNLNSNFVRAVLINTYGSTCTQPFGYEETTTPPPTYTV"
BASE COUNT         2213 a         1839 c         2390 g         3040 t
ORIGIN      
        1 tcactgtgta ggtcggagga ggtgtggttg tttcttcata gccgaaaggt tgggtacagg
       61 tgctaccgta ggtatttatt aagactgctc gaacaaagtt agagtttagg ttagaaacgt
      121 ttattgacgt tcctgttcct acaactgcag tggtttcatt ataccattgg atgctggtat
      181 agtccgtccc tgctatttgt atgacgcttc cactgatgat cacatttgtc agttttggtg
      241 tttttgcgtg tccgtagttc gggtctgctt ggttcgtccc atcgggttcg taggagaaga
      301 agaacgcgcc gtttatcatc gcgtttctga aatcgctttc attgagatta ttcatgaaga
      361 aatgctggta gtttctgaag gcattgcccg ctagggagtg catgtcatcg tcggagaagc
      421 cccagatgag gttgtctgga tctcgttctt tgttgatgct gtcccatttc actcggtcgc
      481 ctgggtatcg gtctccttgg ttgtatgctt ctaccccgag gattgtgtcg tcataggtgt
      541 tgaaataagt gttgtaccaa ctgggggcgt aactgtatct tcctggatgc atgaagatcg
      601 agagtccgtg atggttttgt atgtattgga aagcagcgtc caggtctcca cccgcgtaat
      661 cattgaggta gctgccgata ttgtgtgagt ttgatggttc attggctgag atggcgagca
      721 tgtttaatgt tgggtaatat tctgaggtgt tcgatggtgt cacgggttct tcggtgatcc
      781 atgttgacca gggccaggtg ttatgactgt ggtctgttaa cgcgagaata tcataatgtc
      841 cgatgtcgtg gtagtagtcg atgactgcat ctggggtgct ggagccgtca ctatatgttg
      901 tgtgggtgtg gaagttccct ttgtaggagt tggctgtgtt gaagtttacg tcttcgtagg
      961 ggttgtagat tctcagccat gactggggtt ggatgagttc atagtatgcg ttgtctgcat
     1021 ctcctggttc agttgaatac gatttgaacg taggccacat acttgttact gtaaagtatc
     1081 tcgtggctgg tgcgttactt ccataggatg aggtgttggc aacactgcct gatgcgacaa
     1141 ctcctatttc gttcccgctc ccatctaatt tggttaatga gacatcgatt tttgctgccg
     1201 tggcagttaa tttagtgata ttcgctcgta gtcgatagta cgtatttgag gtaataccac
     1261 tataggtcgt aatcggttgc tggacgcggg cgtctgcact gtttcgccag aaggtgacaa
     1321 taccgccgtc agtatcatcg agttgtacac cgaactggat ctctgaacct gttcctgtta
     1381 cggaagtagc ccatccaata cggtcgtcat caaatttccc ggtactgctg ctttcgaaat
     1441 ccattcggag gttgactcct atgaaatccg ggtcgttcca gttgaacggg tgagcttccc
     1501 aggtgaagat ggttcctgat ggtgcgagtc caatcgatcc tccgacacca tttcctgcgg
     1561 tgatgacagg accgctgcca ccatcgtacc aatcagcatg tgcgccaacg gtttgtcctg
     1621 tggtgaatcc tgtgttaaag gtttccagga tgccaccttg tgatggtggt aatgcggtga
     1681 atgttgcggt aactgttttg ttgccgttca tggtgatagt ggttgggttt atactgccag
     1741 tgagatcgcc actccagcct gtgaacgaat atccttgtgc tggtgtcgcg gttaaggtga
     1801 caacggtccc taaggaatac gtggattgat caggtgtttt tgctactaat ccacttccta
     1861 cggtattgac agtaagcgtg tatagagctg gtgagggatt catggtgtag gagagggtga
     1921 attggctgtc tgcatcggtc tgatactggt cgaggtaggg tgagtatgtt ttgactttga
     1981 tttcgttggt agctgggcag aacgtcatga ttctgagcca accgttacct ccattggggt
     2041 agtcttggta atcagcaagc aatgtgttaa tgacgtttcc attgtaggtg tctgctcgtc
     2101 tgccttcgta atgcatgtga ccgcagagca tgaggaagag atttgaattg tctttgagag
     2161 cgttgtagat gttgatacct tcgcctgtcc agtctccggc agcgttgttc aatatgctgt
     2221 gggagacgac gattgctcga cgagtgctgt aggtttgggt gaggttctct gcccaggcga
     2281 gctcattggt atcagccgcg tagtcaagac cgattgcgat gaagtccatg ccgccggcgc
     2341 tgaacagcgc gtagttgttg tcgtttttcg tggtggggta atgtccgccg tagtaggttc
     2401 gtcctgcgaa tcgggtgtag ttgaagtaag tgttgaagag gttggtggca tgatcatggt
     2461 tcccctggat gatggtgtag gggataccat agggtaattg tgtcgtggca ggatcttcga
     2521 gaagatcgta tgcagcgtca gcacgctgga attcaaggtc tgatccgccg ttggtgatgt
     2581 ctccttcgtt ggtgacccag acgatgttta acgcgttttt atgggcgacg atccattggg
     2641 tctggttcgt gaagatcgtt gggtaacctg aggcatagtt ttgcgtatca ggcaaggcga
     2701 tgatggtgaa attcacttgg attgggagga gttcttggag catgaatgca ccggagtacc
     2761 atttggtcgc ccctgagaca gtgatggtca tatcgccgtc agtaccggga tcgatattgg
     2821 taaatccagc gacatatccg tgaacgctat tatatcctgt gtagtatgat gcggtgtcat
     2881 agggaagggt tgtttgactg tgggtgactg cggttgagga atttttggta aagctggtca
     2941 catcagtgat ggtgactgtt gaattatacg gattgctcga atctgctctg tccgcccagt
     3001 gtacaaaccg atatcgttta ctggagtcta atcctgtgaa ggtgatgatg tggtttccac
     3061 cggaataatg aaggatgcta tctgggttga tttttccgtt gaatatcttg tatgcatcgg
     3121 ttccagatgg gaggacgcct ggtcctgtcc cgtcatttgt tcctccgctt attgagacgc
     3181 tgacggcggt attcgtaccg gtcgcgtaat ctttgaggag accgctgttt cctgaatggt
     3241 agttggtcgc tggtgccgat gaggcagctc ctgctaagtc attataggcg ctccatcctg
     3301 ctgttgttgg gttgattgtg aaggttgcag taaccgcttt gttgccagtc atcgtgattg
     3361 ttgttggatt ggtactgccg gtgagatcac cactccagcc ggtgaagctc cagcctgagg
     3421 caggtgttgc agtgagtgtg acaatggttc cagaattata agtggactga ttaggattct
     3481 tggtcaccga tccgctaccg atggtgttca tagcaagcgt atactggggt ggaaggacag
     3541 tgaacgtgta attgcttggc gcaaacgcat tatgtccacc agcatcatat gctctgcaat
     3601 tccatttata tgatccaggg ttgagattaa ctataaatgt tgttgtatta gttattcctg
     3661 aaattgtttt tacttggtca gcatgccaga cagggattcc acctgttgta taattcacga
     3721 cgagtaacgg agcattagca gattctcctt cataggcctc tgctgtcctg tgtcctgttc
     3781 ccgtgatgat aatgaccatg gaatttcctg aggaccatcc cggtctattg attatttctt
     3841 ggattactgt tgatgcgtcc ggcgtgtgct tcttttgtga ttccgtatcc caagaaggta
     3901 cattattcca agcggcaaat gatgatgttt ttattctact ggtgatatca ctggctgttg
     3961 atgtaaatgt cggggcgtta tcaatcgctt gtccatagat attgaggttc gtagtgtcac
     4021 tgtttaattc gtctacttcg aactcgatat atgcgtttgt gatggtggct cctggtggaa
     4081 tcgccacgtt ttggaatcgc atccctactt cttgatttcc ttgatttgga tcttggatga
     4141 gttctaaatc agtgctcact aaatctattg catgtgtcga gaggtattct tctgcatcat
     4201 cggtccccgt agaaacccgt acgttcacat tcccattcgc aaggtcggta gatccgaagt
     4261 ataaggtgac attatggagg ttcacatcat cggtgctggt gcagttcaat gtgatttgtg
     4321 agctgcttat ggtggcgccg tttgttggtg aattgagggt tacggttggg gcgtcgtcct
     4381 gaacggtgaa cgtatacgtt ttatttgtcc atcgatgact accgaggtca gtggcgttca
     4441 cataccaggt ataggtggct ccaaggttca aagtcgatat tgggcaagtt ttcatcccgt
     4501 tattcacatg gttttcagaa gcgctcccaa tatttggact agtatggatg gtccagttga
     4561 agaaatcatt ctcaggatct gacatttgta ccgttaatgt tgaaagaccg acagggacat
     4621 tgtttgaatt atctggtggg gttggtgatt ggagaaccgg tgtcctgttt cccgtataat
     4681 tataaaagca tatcgcgtct gcgtactgtg gttcaacttc atttgctata cctgtttttc
     4741 ttcctaagct aaagaactga tagtacccag tgctatttgg gaaattgaac tcccattgcc
     4801 agggagcgat tgtatcggtg ttaaatttgt ttgatgttcc ttgattatgt tcttcacttg
     4861 aaagggttgt tgtaatttgc cattcatccc cgtagaaggt gtatccttct gtgtcgatga
     4921 tgcttccggt gctgtcatca ccaggagcag tattccatcg gttccggagt tcttgtccgc
     4981 catcattttc cgcccaatca atttcaagcg cataatatct tccgctattg aggctttggg
     5041 gggtggaatc tctccattgg acaccatgtc cgccgttatt gtctgataca atgagggagt
     5101 cgtttaacca gaggccggat ccgtcatcgc taccggtttt gaagtagagc gtcccggtct
     5161 gtggtgcctt gaagtaactc cattcgttga gtttaatata atcgactgca ccgcatacat
     5221 ccgtgaattc acccgttgga gagtcgccat tacaatcagt atctgcggga actaaatcaa
     5281 catagtttgt tccactatag cttgttgctc caatatagct ccaaccggtg gttgtcggta
     5341 atttttctgt cgctgcgttt acagggtcat agtagaggta gcatgatttt gtttgatatg
     5401 cactcatgtt tatctctgcg agcccttgga gtgtggtgac ccatccgttt tgtgtggtaa
     5461 gggcgtagcc gtcgaagggg atttgtatgc cgttgtctac taatgcggcg tcgttttcgt
     5521 tgcttaatcg tccaagtggg agagttatag tgatgagaac atgggttttg gttcgtgcga
     5581 ttccttgtgg ttctgtgaag ttgatttttg ttcgataatg ataggcagta ttccaccagt
     5641 tgatactgga gccttcccag gtgatattat ccggtgaata gcgataccat agggtgacat
     5701 tatcgagggt tggagaaccc gaggcggtga gagttttagt cggggaaatg atcgcgtagg
     5761 gcgatatgga tgctatagag gtattgattg gttctgtagt gaaggaatag gttttgttgg
     5821 tccacaaggt tccttctctg gtgttgacgc gccagtaata tcgggtggaa tagtcttgca
     5881 tagtggttgt tatcgctgtg atgacaccat ttccattgcc aagggtgcta tgggcgagag
     5941 gtatccaagt gcctgttgca tttgttgaga tccaccattc gaccgcatca tgcgaataat
     6001 cagtgatgga gacagataat gttggattta ggtgaatatt ggtggattgg ttggatggtg
     6061 tcacgttgga taaaagaggt gggcttttcg tgattacaaa ggagatcgtt tgtgatttag
     6121 cgacattatt atatggatct gtcgcccaaa tgaagaaatg atatgttccc acggtgaagt
     6181 acttttggtt gtaataatat gtattcgggt ttattttatt ggtgataatt gaataattca
     6241 ctatgtttgc attgggatct gagatattga tacgaacatc ttgtactcct acattatcct
     6301 ggacgttgca ggtgatattg acagggattc cttgaatcgc tgtggatggg gtgacgagta
     6361 tattggcgat ctgcggaagg ccggtatcta caagtacggt gaattgggag gtagctgagg
     6421 tattggtatt gccgttaata tctttacacc agatggaata actgtactga ccaggcaaag
     6481 tgtaggattc tatcgacgta taaagggttg gttccaaacg tgtcgcgtta atatagattt
     6541 gatcaagata gacatcactc ccggtacctc cagaggcaca tcggaaacga atattcaaat
     6601 tcgatgggag attgtaggta gatttgttga tccagaccgt gtcatggaag aagagccaat
     6661 ttgcgaattt gtagtaggtg ttagtgtata tagctggggt gaataatcca gtgttttgtt
     6721 cgtatatttt tcttacttgc cattgggttc catcataata ttcaagataa tatcttccac
     6781 cgtcacccat tccatatgca ttcatccaga aatcaatttt aattgcgttg taacttggtg
     6841 tggcgatatc aattgggtgg gtaagattaa atgatgaggc gacccctgag ttagcttcaa
     6901 tgtttgcgac gtaactccct tgatgtacta atcgccaatc gccgagatat aacccacaat
     6961 tcgctccacc agcggtgtaa ttaccccact tgttattttc aaaattatca taggtcaatt
     7021 ttgtccattg atatctatac aaggatttgt ttacgtaaga tccgttagga tagtttaggc
     7081 gtaattttgt ttcagccata tgaacatctt cagtgattct tgcggagatg ttcacgtctt
     7141 gcccttcgaa tggtgtctgt gatgagatgt tcacgctctt tatttttggt gcagatttcg
     7201 ttttgaagtg gtaggtttta ttggttataa gattatctgc aaggggatca acagcgatga
     7261 ctcgccagta atacgttgta tctaatttat tcatcatagt tggtgtcaca ttgtaccaac
     7321 cgtttccaac gtttgtatat gatgcgagtg taacccatga tcctgatgca ttcgtacaaa
     7381 cgatgatgtt cattcgatca taatggaaat caatagcatg aacagacagc gttggattta
     7441 actttacatc taatgaacca tttggtgatg cttctttgag aatcggttgt ggtgcgcctg
     7501 gtggtgggat atagacggct gcacctgttc ttctttcgct ataacccgca ttgacacttc
     7561 ttactcgagg agttggggca ctaaccgttg tttcataagc tacaaggtga ttctcagaaa
     7621 caaataccaa ttcattttgt ccatcgttat caatgtcttg gactaatgtg catctctgca
     7681 aaggtagatc aatggtatca acaatttgat acgtgctatt atagattgtc agcttatccc
     7741 accaggttgc aagtatttcg agtttgtcgt caccaatcac attagccata tagggtgctt
     7801 ctttacaggt aattaaggta gcatcaactg tcttggttgt aagatcaaaa actacacatg
     7861 gagaactaga tgcgataaga atctctaaat gtccatcacc atcaatatca tagacagaat
     7921 tcggtgaatg atttggatat ccgccactat ttactatata ttgagcggtt tttccgttaa
     7981 taacctgcac aggtccattg ctatacgctg ttgtgacgga atctaaaacc ccatcgttat
     8041 tcacatcgac gagaattggg agttgaggat ctcctctggc tcgttgatac catataagat
     8101 taagatcttt atcataacag tgaattcctt tccctggttc tccgttgtaa tccgctagga
     8161 gaatttcaat attaccatcg ttttcgatat ctgctgctga aagcccacca tagcagggat
     8221 aatagataga gaccgcttta acaattgcac catcggttcc tctgagttta caaagtctac
     8281 ttccaccata tgcatcagag gcgacatata catacggata tccatttccg tctacatcag
     8341 cgatcacggg atgttttcca tcggatggtc cttctgaaat ccaatatact gaaccatcgt
     8401 cagcatgtaa ggcaattgta gctccgaatg gacctgttcg attacaactt atcacaattt
     8461 cctcgatacc atcattattt aaatcagcaa gctcaaacgg tgagtgatcc ataatcagat
     8521 tatggctata ctcccaaatg aggttgccat ttaatccatt cacgcatata gcttttcggt
     8581 ctcctgttaa gaaaatctca tggataccat cgtgatttac atcagctgaa agtggaccaa
     8641 gtgtttgtcg gcaataggtg ttactgtacc atttttgtat aaatgcgtat gctgtaaaac
     8701 tatatgatct attaatccat gatgaaccat aggaaacatt gatactccag taataggttt
     8761 gatctttcac atccatattt tgggtggttt gggtgtacac tccactatat ccatggtatg
     8821 tgttgattgt ctcccataca tccatgattt tttttctaaa aatcacggta agataattag
     8881 acgaagtatc ctgtattttt acactcaaca caggattata aagagcgtcc tgatcgtccg
     8941 gatacacttc agaaataata gggtagccta caccggtaac aaaccaatat gtcttatttg
     9001 tccactttct agacccatac aagtctgttg catttacact ccaccaatat tttgtatttg
     9061 gtgtagtgat attttctgta gcatctgata tgataccaaa accattgtaa atcgtcgtgt
     9121 ttctcacctt atgccaacta ccacttttat ttgtccaaaa ctcacaatac acattatcgt
     9181 tgttaagatc ggtaactgtt acttctaggg ttggattgaa ttgattatta accgatgtgt
     9241 gcactggttt ttcatgagat attactggtg gaaaaccact ttgattctcc tcgatgctaa
     9301 gaaggtggaa ttttgaagga gtattcagat tgttataaaa agtttttatc catcctgctg
     9361 agaagcaggt gtttgcgatt tggacttcat cgacaactcc tttgaagaac atcgcattat
     9421 ttccattgtc accgatatgc caattggcgg tggtaacgga gagtgaaccg gttaaggctg
     9481 tg
//
LOCUS       DATMXJ010000012        32794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_47152, whole genome shotgun sequence.
ACCESSION   DATMXJ010000012 DATMXJ010000000
VERSION     DATMXJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 32794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32794
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47152"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VN377_00285"
     CDS             <1..604
                     /locus_tag="VN377_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR26853.1"
                     /translation="CTGTEHKKLDVPGESEFIGKGVSYCATCDGFFFKGKCVVVVGGG
                     NTALMESIFLKKLGCKEVTVVHRRGQFRAEKVYEEEAREKGVHFLFNTIVEEIHGKEN
                     VSSVSLFNLETSARSELLVEGVFASVGVLPQNEIAKALQLKTNDDGYVVVDSEQRTSV
                     RGVYAAGDITGGLRQVVTACAKGAVAALSSTEVLGKKYPY"
     gene            complement(598..1044)
                     /gene="hycI"
                     /locus_tag="VN377_00290"
     CDS             complement(598..1044)
                     /gene="hycI"
                     /locus_tag="VN377_00290"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HWR26854.1"
                     /translation="MKDILILCIGNQEGGDDGIGPYIAERLKQESSHDMVLDCGTIPE
                     NYTGVIKRQKPKTLIIIDAAEMNLSPGEIRIIPREKLGTMHLSTHGIPLSVFIKYLEN
                     EVPCIIFIGIQPETMQGSLSDPVKKSGDDLIKLLQKKKIEQIPKLQ"
     gene            complement(1063..1323)
                     /locus_tag="VN377_00295"
     CDS             complement(1063..1323)
                     /locus_tag="VN377_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26855.1"
                     /translation="MWQWQDIILTIINFGFMITAIPAIFRNYHHKEAKSQSLSMYLIT
                     AILLTVMAYVFLTLDMLLSSISTAGTGIMWFLLTYQKIIYSK"
     gene            1406..2458
                     /gene="hypE"
                     /locus_tag="VN377_00300"
     CDS             1406..2458
                     /gene="hypE"
                     /locus_tag="VN377_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWR26856.1"
                     /translation="MAKKITLAQGAGGELMDQLIKQHILRFFNLQDAAEVPLTALDDA
                     AVVDDIVFSTDSHTVQPLIFPGGDIGSLAVCGTINDVSVVGGSPLALSAGFIIEEGFS
                     LDTFETIVKSMGRTANKAHVPIVTGDTKVVEKGALQQFMINTSGIGKRSPALERNIKE
                     VKRYRKFRSRWLLDSNLSAGDTLILSGNVGEHGIALLSFREGYGFETTIHSDIAPLNG
                     LMSVLLAEGGIVSAKDPTRGGLANTVNEFSEKSHVGLLLYEDAIPIPKGVQSACDMLG
                     IDPLEIGNEGKVVLGVVKEKADDVLAMLKKHPLGKHAAIIGEATDKVSGVVLQTSVGG
                     KRILRKPLGDPIPRIC"
     gene            2513..2968
                     /locus_tag="VN377_00305"
     CDS             2513..2968
                     /locus_tag="VN377_00305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWR26857.1"
                     /translation="MRGEYGIAKGLVKSFKKALWVYHINSGSCNGCDIEIIASLCPRY
                     DIERFGIKLVGSPRHADVLLITGPVSRQMVDKVKRTYEQMPDPKAVICVGNCGNTGDV
                     FYESYNLVGPVDKILPVDVYVIGCPPRPENIIHGITKVWKKLETLETTA"
     gene            2965..3489
                     /locus_tag="VN377_00310"
     CDS             2965..3489
                     /locus_tag="VN377_00310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWR26858.1"
                     /translation="MIKPEDILGSFQTNFPNSITEPRIERRTGGTLKTEIVHLWFHIE
                     ARFLKDAVKHLFTFEQYPHFAVSSGYDLGDTIELVYHFSLFHGERGREISLNITVSLP
                     KENPVIDTITDLIPGALISEQEKQEMLGVKIHGIPKDVRVFVADDFPKDVYPWRRDET
                     GPAKLVRNLHEGES"
     gene            3486..4712
                     /locus_tag="VN377_00315"
     CDS             3486..4712
                     /locus_tag="VN377_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWR26859.1"
                     /translation="MTTTSKTTYQIPIGPIHPALKEPVNFTFEIDGEKIINVDVHLGH
                     VHRSIEWAGTKRNFIQILYLAERICGICSYCHPMSLALAVERAAHIQVPDRAEYLRVV
                     HAELERIGSHILWAGVAAHEIGFDSVLFLTWQVRETVLDLTEYLTGNRITKGITMIGG
                     VRRDITTEMVPRIHGTLQDLKKQMEKLHHVMLDDSTIQMRCRNCGVLTKEDALKLCAV
                     GPTARASGVRKDVRLDQPYFAFGDLDFEYTTPDVLTNEVHGDVYDRIIVRLLEVKQSI
                     DLIEQCLDRMPAGDIVAEPEIPALLAALKKVTGEGIGRMEAPRGEVFHYVRLAGSESP
                     YSWKVRAPTYNNILPWIPMLLGQQIADIPIIAASIDPCMSCTNRVTIVHHNQPTMVDR
                     EKLHQLSIEKTRRLCL"
     gene            4703..5110
                     /locus_tag="VN377_00320"
     CDS             4703..5110
                     /locus_tag="VN377_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR26860.1"
                     /translation="MSIRYTMIPQLLSQLFKKPFTNRHPKKYIPSSSTTFFTDVQNGK
                     ETMNPPVETPEGFRGKIVYEKEKCIGCKLCLKVCPSEAIIFKDEEKKIKIYLARCTFC
                     AQCNDICPVNCLHMSNEFLLADTEKYSPSLIVE"
     gene            5154..5384
                     /locus_tag="VN377_00325"
     CDS             5154..5384
                     /locus_tag="VN377_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26861.1"
                     /translation="MGKNRDIAKVLLLILIGMFVPFLGSITLSYGFVPRTIIVTFLYF
                     LVIFGLELGFVYLYFTFSGRRANKKMEEYKPK"
     gene            5491..5658
                     /locus_tag="VN377_00330"
     CDS             5491..5658
                     /locus_tag="VN377_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR26862.1"
                     /translation="MVVKVDKATCTGCAACASACPVEAIKVTEKAEVNEDTCIDCGTC
                     VDECPVQALSL"
     gene            complement(5674..6366)
                     /locus_tag="VN377_00335"
     CDS             complement(5674..6366)
                     /locus_tag="VN377_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWR26863.1"
                     /translation="MDYDEIMSKLRSLANPENVKGMTRYGINQHNNLGISIYILRPLA
                     KQIGINHALALKLWDSKIHDARLLACFIDDPQQVTSEQMDAWAEDFDSWDICDQACTN
                     LFDRCPLAYDKVFQWADKEKEFVRRGAFSLIAGLTVHDKTATDHDFEDFLPLCITYST
                     DERNYVKKAVNWALRNIGKRNLSLNKKALKTAEEIKNINSKSARWIAADAIRELSSPK
                     VREKLERKKKKK"
     gene            complement(6396..7019)
                     /locus_tag="VN377_00340"
     CDS             complement(6396..7019)
                     /locus_tag="VN377_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HWR26864.1"
                     /translation="MNDLLSVINDLKHSNTQRKIHRRIQEFKTIDTHSAEELFQELTF
                     CILTANCNAERTIHIHSQLSECFCTDTKETLAKKLRQHGYRFPNTRAAFIVESAKKKD
                     ILPTMIQTLPHEQRRRWLVDNIKGLGYKEASHFLRNIGYDDYAIIDTHILDILARYRI
                     IKQPKKLTKKNYLKIEGHLQKIARNTKLTLAELDLYLWFLETGKILK"
     gene            7361..7696
                     /locus_tag="VN377_00345"
     CDS             7361..7696
                     /locus_tag="VN377_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWR26865.1"
                     /translation="MKRVPCEYVIWNGLPLIRKEIAESLMTNFGLSQKQAAEKLGITP
                     AAVCQYVSHKRGNKTIDDEELKTEIKLSAQRILNYTDDAMIKETCRICKIFMAKGIFP
                     SGCDSCPPE"
     gene            complement(7700..8644)
                     /locus_tag="VN377_00350"
     CDS             complement(7700..8644)
                     /locus_tag="VN377_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWR26866.1"
                     /translation="MDLVAITFQSVAILLGIGVFGFLIVQRRVVAENALGALSNLALD
                     LALPCLVFVNIVSNFSPQEIPDWWVLPLWWVVFTGLAALLTGAFMFASKKTTRREFAM
                     SLFLHNGLFFPLAILTGMFGASSPYLVSLFLFMIFYPAFLFNIYPYFFGKNERKINWR
                     RIFNIVFISTILALMIRFADIQSYVPGMVLTILTMLGAMTVPLLMLVIGGSLSLDFQR
                     KGKIYSTEIAKFVLIKNLLFPLIFLAALLVIRPSYQIGLLLILQSAVPPVTALPIFTK
                     RVGGNESIVNQFLVASFICSLGSIPLVISLFTVFFPPG"
     gene            8790..9224
                     /locus_tag="VN377_00355"
     CDS             8790..9224
                     /locus_tag="VN377_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWR26867.1"
                     /translation="MRTTSILDLMTADHTKILKLLGDVEKSIGIELVSLMKVFDTFEW
                     ELEKHIFTEEKAIFSSYNPRNISEGYKMVPELIQQHNEILNRIRVMRKDLMWNKPVQY
                     PEFKELIMAHKTFEETSLYPKLDQELTNAQKDEIIKKIREIV"
     gene            complement(9237..9767)
                     /locus_tag="VN377_00360"
     CDS             complement(9237..9767)
                     /locus_tag="VN377_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HWR26868.1"
                     /translation="MTGKESIIEAMKKNAKDNGYYICPDQQLHNDLIDGLATNTARYG
                     YGSCPCRVASGAKEYDVDIICPCEYRDADVDEYGMCYCGLFVNEKIKNNPSLLGPIPE
                     RRPQEIIDVVLAAAEKKPNVPKEITDQPIARKKTGAVRVWRCTVCGYLCAREIPPPIC
                     PICKAKAERFEPFTMS"
     gene            complement(9764..10051)
                     /locus_tag="VN377_00365"
     CDS             complement(9764..10051)
                     /locus_tag="VN377_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWR26869.1"
                     /translation="MKRETIPGKNTTHHVKVYTLSTCGWCKKTKELLKALDISFEYVD
                     VDTLTGNDYIEATDEVKKYNPYRTYPTIVIDHGKHVILGFKDKEIMEKLRS"
     gene            10146..10340
                     /locus_tag="VN377_00370"
     CDS             10146..10340
                     /locus_tag="VN377_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26870.1"
                     /translation="MTVNYYQCQTCKKKTIVKTKKEKPSCCGKPMKKISVDICLQPDH
                     AEHSRPMGEDEPCDDFRGGN"
     gene            complement(10482..10880)
                     /locus_tag="VN377_00375"
     CDS             complement(10482..10880)
                     /locus_tag="VN377_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWR26871.1"
                     /translation="MKELHISSQRRNQMIDITDEVQRLVKEENINEGSLIVYVPHTTA
                     GITINEGADPDVQQDIIETLEKLIPENASYHHSEGNSDAHIKASVLGSSVTILIAKGK
                     LVLGTWQHIFFYEGDGPRHRTVYISTNKNK"
     gene            10935..11567
                     /locus_tag="VN377_00380"
     CDS             10935..11567
                     /locus_tag="VN377_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWR26872.1"
                     /translation="MYHCLVQQLLKTMKLAPSAILFDMDGVLVDSLDSWWKALNSALQ
                     RFKHQEITREEFIITYWGHGLKANLERLHLNPEVARFCNITYGNHLDLVTIYPDTENT
                     LRTLATYKKVVITNTPSDCARQILRKFHIEQYFEAIITSDDVVRSKPDPEIVFKACER
                     LGVSVQSVLLIGDTESDVKAGKAAGCTVVGVNIPADFMIQRLSELPSLLR"
     gene            11646..12407
                     /locus_tag="VN377_00385"
     CDS             11646..12407
                     /locus_tag="VN377_00385"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWR26873.1"
                     /translation="MEEDIQIIEYKNIDLSNSVLIEAFPTVGLVSSIAGHFLVEQLNL
                     EEIGTISSRYFMPAAVIHNGMPSPPVRIYASKKICGSNGDCDQIVVIISEFMPSVDII
                     KPVASMILSWAQKKKCSHIVTLEGTHGLNPKKPKTYGVATTQKMKDILKKYSIEETKE
                     GMITGITGVLLFEGARLKQDVLCLLAEAHSSYPDSRAAALLVETLDKMLPEIKIDTQP
                     LYKEADDIEQKIRSFIKQAQPTAPSSVPLQPQMYG"
     gene            complement(12416..12976)
                     /locus_tag="VN377_00390"
     CDS             complement(12416..12976)
                     /locus_tag="VN377_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWR26874.1"
                     /translation="MDVKEAIRNRRAYRSLDPIEITEDLIRDLALHAQLSASCNNNQP
                     WRFVFVYEREMLKKMHEAISKGNEWVQRASMIIVVLSKKEYDCIIGERVYHQFDTGMA
                     TAFLILRATELGLVAHPIAGYSPKKTREILGIPEDLEVITLINVGKHAATIYPILSEK
                     QAAIEKKRPERFPLEKFVFLNRYTNK"
     gene            13097..13339
                     /locus_tag="VN377_00395"
     CDS             13097..13339
                     /locus_tag="VN377_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR26875.1"
                     /translation="MAIGFALLSISPLHEKTVYEKLTHISEIIEVHPLFGEFDILVKI
                     QASDIDSIGSIVINKIRSIQGVMDSKTLIGTKSLSG"
     gene            13500..15566
                     /locus_tag="VN377_00400"
     CDS             13500..15566
                     /locus_tag="VN377_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR26876.1"
                     /translation="MKRRYLTTFLLVLLLLSSISTAVTQKWTKDTSTLPKNTIYVDDS
                     NTQGPWNGSSEYPYQSISDGIRHATDGDTIYVFNGLYSETIIINKTISIRGQEQENTI
                     IDGYNNGSVVTVASEDVYLRRFTIRNSGGYQGDAGIAVLANTTTITECTIYRARAGIF
                     VQKQSETTITNSRFHTNGFGIVSSSSAFVTIDQCTFYHNGAGVYLFNSTCVTIAHSYA
                     DTNGIGFLCERSSHIDISESAARDNDDNEGGMFFVDSQYINILNSYIVHNGVGVNLVN
                     SSACYIDSCNFSLSTHYSCKLLKSGSSILLTNCIFTKSLRYGLFAEESAFTISWSNIY
                     GNENFGLYATSSAIDARYNWWGAKTGPAHNGLVRADRGTWNPREISYRPWLTFPMPEI
                     GPDWDLEKTFQKPTYADPWPEQILFPGLDTDADGAPDWWEVKWGYSPTVWDDHQHLDP
                     DNDSLNNIEECYMDQFGSNPFAKDLFLEFDWTKSIQGNSTNKPPEEEITQMIEAFARQ
                     NITLHVDTGELGGGEELPSQTHVSYAEIIDIYWNSFLHTDLNNPRQHIFHYGIICDYS
                     ESGGFAIMGWNHLNAFIISTQLLAEKYSYSTKGWLATAASMHEVGHTCGLIATTFVGI
                     DNRNTVNPFYKEFWAYLPYKSMMNYHYTYSFLDFSDGSHGRGDFNDWANLDFSFFKNT
                     DFSYPV"
     gene            complement(15567..16676)
                     /locus_tag="VN377_00405"
     CDS             complement(15567..16676)
                     /locus_tag="VN377_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR26877.1"
                     /translation="MNLKIVGIDFLFSIRSWFRSRGTVFWSLLFPVMLILLFGAIFSG
                     MGESKYTLYVQDLDNTGMSQSLVTILNKTGVLTIKNVTTNETTADYVKENNIKNLIVI
                     PQGYETSITRSFADPSIKVNLTYYFDPTEQQTNQVLRSVISSVLQGLNMQLSNGRTVV
                     GLDEQSTVTENFGFIDFFIPGMIGFTIMQQSIYGSIERNTKFRKDGILRKLLTTPITR
                     SEWILAKMLFMLFLAFISTSVAIGVGILAFGIKVNINLLTIIIIIATSFLFSGIGMII
                     GRFVKEEETADMAAGAISFPMMFLAGTFFAVDAMPSIIQVIAHALPLYYVNEGLRNAM
                     IYMNQTEALINGGIVVIFAAIFFIAGVLLTKWKED"
     gene            complement(16673..17737)
                     /locus_tag="VN377_00410"
     CDS             complement(16673..17737)
                     /locus_tag="VN377_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR26878.1"
                     /translation="MTEQRFKCPNCGEIATFYGDPGETKVVTCAACHMKGRLTILGDK
                     QDNTVAIEVSHLKKVYDDLIAVNDISFAVKKGEIFAFLGPNGAGKTTTVEMIESIRTP
                     TTGAIKLLGVDTKTGFNQIKERISILPQEFHSFERFTVRETLTYFSKLYKKRANIDEI
                     LDAMDLRSHEKKLYRNLSGGLKQRVGVALSLVNDPEIVFLDEPTTGLDPKARREVWQV
                     IANLRNKGKTVFLTTHYMEEAEHLADHIAIIHKGKIIAEGTLQDLLNRYGEKTTLYIK
                     NCHSDEARQLLEEKGFEVHSSGNGNLSIKIDYKEKVLEVLSELRHECIEYDGLDIRGS
                     NLEEVFLQLTGARLIEEVET"
     gene            complement(17801..18490)
                     /locus_tag="VN377_00415"
     CDS             complement(17801..18490)
                     /locus_tag="VN377_00415"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWR26879.1"
                     /translation="MLTLATLVLVRHGQSQWNLENRFTGWVDVPLSPKGREEASSAGK
                     KLKDIRFDTMYVSHLMRAIQTLHYILLELSDERIPILYHEEERIHAWEHYTGTRDKEI
                     PIYQSVDLAERYYGDLQGLNKAETMKKYGEAQVHIWRRSYDINPPHGESLKDTCERTL
                     PYYKKNIIPELQAGKNVLIVAHGNSLRSITKYVENISDEEIPKIEIPTGVPIVYTFDS
                     QMKQQKKILLQ"
     gene            complement(18495..19997)
                     /locus_tag="VN377_00420"
     CDS             complement(18495..19997)
                     /locus_tag="VN377_00420"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWR26880.1"
                     /translation="MKESLDYIYKEQKEVSLYGGIAALLGWDQMTYMPPKGSEERADQ
                     LSLISRLAHERVTSETLYTHLKKLDDVANTLSKKDHLVVTKLYRDVEKARKLPAEFVE
                     KMTKTTSIAYGVWQEAREKNKFSLFQPHLEKIVELEKQYYDYVKLPGHPYNTLLDDYE
                     EGMTVDVLQKEFTILRQQLVELLQTITSSAMYQKQKPVKLIMESNCQKKLCSILLTHM
                     NLPFDRTRLDVSTHPFTTSMGDDDVRFTTNYEREGFLSSFFSTAHEAGHALYELGILK
                     GEYKDTVISDAPSLGIHESQSRFWENMIARSKPFWTYFSPIFQKIAPNQCKTMNTVTW
                     YQAVNQVRPTLIRTEADELTYCLHVILRFELELDLLEDKITVAELPACWNQKVTEFLG
                     IAPKNDREGVLQDMHWSGGDFGYFPTYAIGTIYASQLFRQFQKEHQTVQEEISRGDFT
                     TILQWLSEHVYQYGSLMTADEIIQKTCGEGLNSKVFIGYLKEKYYPLYGV"
     gene            complement(20014..20778)
                     /locus_tag="VN377_00425"
     CDS             complement(20014..20778)
                     /locus_tag="VN377_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWR26881.1"
                     /translation="MKLLCWNVNGIRAIDKKGFFTWFLKESPDIMCLQEIKATPEQLP
                     PHLRNVPGYYTFWNPAEKKGYSGVATFSKQKPLSVKNGFGREEFDTEGRILITTFPSF
                     TLFNIYFPNGKKNKERLQYKLDFYDEFLSYADNLKAQKQNIIVCGDLNTAHKEIDLAR
                     PKENEHISGFLPVERAWIDTFIDHGYTDTFRRFHPEPNHYSWWDLKTAARTRNVGWRI
                     DYFFVNKEFLPKIKNAFISQEVMGSDHCPVGIEITV"
     gene            complement(20856..22076)
                     /locus_tag="VN377_00430"
     CDS             complement(20856..22076)
                     /locus_tag="VN377_00430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWR26882.1"
                     /translation="MSTTLRTTLQELTPAMIETLRAVFWQNVFATKAIEHTLKLHPEW
                     EESKRAVFSDTIYDLIRWWRPLWYIIDTEPSADEKELQNLITIYLFSKKGDLSALQKK
                     KDLDATQLLQRIKTTKISRALRESVPDWLDAQGEKELGMRWEPVLVSLNKKPELVIRA
                     NTLKTSPKELLVILRKEGIAAEPIDGNPDALFIKEKINVFKLHSFNAGLFEVQDPASQ
                     MVSRILDPQPGMRIVDACAGEGSKTLHLAALLKNKGKIIAMDTQEWRLRELRKRAAKA
                     GVDTIETRLITTSKIYKRIKETVDRLLLDVPCSGLGTLQRNPDIRWKLTAKDLERLRD
                     LQQDLLEKYCTLLKPHGNMVYSVCSILPSEGEEQIQRFLKNHSEFRLLDEKRTWPDTD
                     RTDGFYIALLQRTS"
     gene            22434..22655
                     /locus_tag="VN377_00435"
     CDS             22434..22655
                     /locus_tag="VN377_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26883.1"
                     /translation="MCKQKFHLQEHQSGLVFDDKFFICEDCRTNTPEQEILGWSQSTM
                     RSSASAMPISLWLIQEQNKDKPPFSRRKE"
     gene            22732..23514
                     /locus_tag="VN377_00440"
     CDS             22732..23514
                     /locus_tag="VN377_00440"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HWR26884.1"
                     /translation="MFIIKLGGSVITNKAKECCFKQGIMDQLAAEIKHANKQVILVHG
                     AGSFGHILAKQYKLNDGFRQKKQVEGFALTQAMVQRLNNLVLASLHKHDIPAVSIPPH
                     AILSLSDHNLARVDYNIFKKYLALGFMPVSFGDVALDNQLGFSICSGDLLIQLLAAEF
                     KPEKVIFVIDEDGLYSANPKTNPNAIFFDNTTVKELEKLTTSLDAHADVTKGMKGKIH
                     TIKQIAKTGVDTIVLNGNIHNRLYDTLKGKKVRSTIIYGDKI"
     gene            23511..24557
                     /gene="fni"
                     /locus_tag="VN377_00445"
     CDS             23511..24557
                     /gene="fni"
                     /locus_tag="VN377_00445"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HWR26885.1"
                     /translation="MNQTEERKREHVQISLKESVSAHYNYWDDVHLMHNALPEINEND
                     IDLSTQLFEKKLRAPLIISGMTGGYPEGKKINEHLAAAASTFQIGMGVGSQRAALENQ
                     KLAETYSIIKEYDIPLKIANIGASQMVLWDRQQMLENAGKIVEMIDADVLAVCLNFLQ
                     EAIQPEGEAQAKGCLATIDMLSREFSLPIIIKESGAGISGKIAHQLKKTKIRGIDVGG
                     AGGTSFSAVEYYRAKMKGDTHDMRAGQTFWDWGIPTPTCVLEVGKATSWELPIIATGG
                     IRHGLDIAKALVLGASAAGMAHALLKPALKDKKSLEQELDVITRELRIAMFLVGVDTV
                     EKLSTVEVRYGSTD"
     gene            24541..25524
                     /locus_tag="VN377_00450"
     CDS             24541..25524
                     /locus_tag="VN377_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWR26886.1"
                     /translation="MDLQTELVKRAAIFNTYWQQYLQIKKPTKLYEASQHLPAAGGKR
                     IRPFLTMTSCESVSGDILKALPLATGLELIHNFTLVHDDIMDHSLLRRNLPAVHVKFG
                     EPAAILAGDLLFAKAFEALLNTSVDFSTYKLLQQDFIDGIIAICEGQQMDMEFERRKT
                     VTEQEYLNMIGKKTGALFELAGKGGGLIGGGNPSEIAALKTYGMTLGLAFQIWDDYLD
                     MSSTATTLGKDIGNDIRNGKKTLIAVHSLMHATGKHKKLLEDVFGNHAASEHDVTLVY
                     DLFKELGSIEYAQRRALQMVNQAKDAIKPVKSSDAKELLYQLIEYTIQREK"
     gene            25527..27242
                     /gene="gltX"
                     /locus_tag="VN377_00455"
     CDS             25527..27242
                     /gene="gltX"
                     /locus_tag="VN377_00455"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWR26887.1"
                     /translation="MSAQRIQREARKFALQNAVSFNSKANEKAVVGKVIAALKKDGVT
                     PAEIIPIVSQVVAQVNTMSAADQLAELTTLAPELLQKEKKERDFSLPPLPFAEKGKVV
                     TRFPPEPNGYLHIGHAKAAIIDSEYARLYDGKFILRFDDTNPENALLEFYDAQKEDLH
                     WLGIQWDVEYHTSDHLQTHYKLAEQLISQRDAYICKCTPEVIREGRYHAKACTCREKL
                     AGPVGLDLWKQMLASRETNLILRLHGEMDSENTAMRDPTLFRVINTEHPLTGDTYHVW
                     PTYDFAGAVEDSISGVTHPFRTKEYELRDQVYFRILSLLNLRAPHLMEFSRLSIDGMP
                     VSKRKIKPLIEQGLVSGFDDVRLPTLRGLKRRGIVPEAIKQFVLQQGFSKVESVVDFS
                     LVESVNRKYLDSRVKRYYFVPDPVQLVVNEAPKKKVKLSLHPDATMGDRTIHTASTFF
                     IPRQDVQVMKIGDVFRLKGLYNVKITKIGETLHGDYGGEELIPETAKIQWTTDECIPL
                     KVFVPGLIFKGDIFNPDSLHELQGCAEKAVADVPAGEIVQFERVGFVRLEKQKTGCVG
                     FFAHK"
     gene            27399..28073
                     /locus_tag="VN377_00460"
     CDS             27399..28073
                     /locus_tag="VN377_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWR26888.1"
                     /translation="MSIVVIMLACFFLGSTVVCRAAPELQPDIDMTTIAVIITVIALL
                     LAFFFLMGGVKYVTPENVLDTEIRKNLYSYIDEYPGSHLREIARELDLKPSNAAWHLR
                     KLEQTNLIRSRAIGGKKVYYLVEGGIEAKQRAISESIMRNKNARDIMAYLSEHPGKHL
                     LEIAHALAINHHVVKWHINKLYEAELIEGDTTNSAYPIYYPTEIGRQFMETYTEFLKT
                     TVENAA"
     gene            complement(28070..28342)
                     /locus_tag="VN377_00465"
     CDS             complement(28070..28342)
                     /locus_tag="VN377_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HWR26889.1"
                     /translation="MGEVIALIRLMPDGVISDKDMDLITEEVKKVVQKPARLGRVEVK
                     QIAFGLRGLDVTIAVPDVEGGLDPIVEKFGKIKKVENVEVVDVGRI"
     gene            complement(28355..28519)
                     /locus_tag="VN377_00470"
     CDS             complement(28355..28519)
                     /locus_tag="VN377_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HWR26890.1"
                     /translation="MKKADRCISCGKGLLEQGSTTFQCPTCDTMIGRCANCREQSVDY
                     LCPKCGFRGP"
     gene            complement(28579..29667)
                     /locus_tag="VN377_00475"
     CDS             complement(28579..29667)
                     /locus_tag="VN377_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HWR26891.1"
                     /translation="MTDEKKKWNKTKISIFVSIALSLSIIVFILYFTIDAQTIQYLSQ
                     VSFRGEFFLFFSIAIALNVLYWFIWGARLKVLSKAIDSKVHISLWEATKIVIANQFLA
                     GITPSVAGGEPVRIYLLNKDGLTTGGATAAVLGERLIDAIFILVIVPFGFFVFKERID
                     VKLISYGLIIGIIIFMSGIILFALALKYPEKTKALLLRFSNRLSRFSKKPERSKKVVH
                     RIEHEIDNFHTSMVLFLTKGRKSFLTAGGLTVLMWSTGFMIPSMILLALGLPPFFIES
                     YAAQALLLIIVMLPTTPGSSGVTELGMAALYGVLLGATHQYLLGVFVLLFRFISYHMN
                     VICGAVFQYRIFKSITSFSLETIEKKEG"
     gene            29779..30729
                     /locus_tag="VN377_00480"
     CDS             29779..30729
                     /locus_tag="VN377_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR26892.1"
                     /translation="MKKLPVKKYLQTMRYAATNYLAKECIYPFYASFKVTHVCSLKCD
                     FCNVWREKTSDLSKEDVFKVIDNIANSSIIVLSLEGGDPLVRKDLGEILQYAHQKPFY
                     LFFTTNGHLLDKRPMKEYGKHIDYLHISIDEGHGNLAFFDRLEEFQSYGPEICIQIVV
                     TRETLPALEEKVKRVYEVNARTVVMPACHLDGTDDFYPDPKQFQGEILRLKQKYPNTI
                     TTPKGFLDNINRPHGCSTSSVIIDSDGGLFYPCRTVGKHLYNFTEGSFMEFLRTPEAK
                     QARTAMDQCDRSCGWYQYFATDVFASPRSLYSSISPYILK"
     gene            30800..31972
                     /locus_tag="VN377_00485"
     CDS             30800..31972
                     /locus_tag="VN377_00485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR26893.1"
                     /translation="MKILFTTESYYPIIDGGAIAQHRIVHELIKKGHDVRVIAPAFSF
                     KNIVENENGSTIYRPRAFILPFYMNNKYHFAPFPFLYVKKIIEGFKPDVINVCSPYPI
                     SICAMILAKKKHIPLVGSIHILPENVLAPFFSSSWYPTLVKYIWSYLIYFYNRVDWAT
                     VPTQTGAVMYQEKGLKTPVSPISNGVDTEMFKPTNKGEYLRKRFNLPEKPLILYTGRI
                     NQEKNVDVLVKAIPFVLKNVDAHFLFCGSGGLKPDMMKLTQQLGVDGHTTFIDFLDWA
                     DYPNIFTLADVFVMPAESELQSIVTLEAIATGVPPVVVNKGAVPELASAQNGLVFEPQ
                     DSNQLAKNIITILSDKQLRDTMKKNSLKLSEQHSMDIVGTQYEQVYEKVLNLPRES"
     gene            complement(31969..>32794)
                     /locus_tag="VN377_00490"
     CDS             complement(31969..>32794)
                     /locus_tag="VN377_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26894.1"
                     /translation="LYSIPMLLFIGFGCLGVEYLKHVKNHWFFQAWLCAILSSFIYSL
                     VTVNTTLFPDRHVEYLTVPACLFAAVGVLYFFRSREHKVSLILKKQLSYPTIQALFVL
                     VVCGLVFSNAVAVYPVYTSLEWMDESIPNETANAIQWIHDNLDRNDSLVATDLRLSKM
                     IWAEGIDATFEGTNDTWTCDTWVGCITDFDGESHRVVTHVLIDDVMRDISVNVRVLQS
                     VYMTNESFLKFSQEPFELVYRNATVNQDNEEIHWAEVYSVNWTFIEQHLMVKKTDP"
BASE COUNT         8789 a         7295 c         7632 g         9078 t
ORIGIN      
        1 ctgcacagga acggaacata agaagctcga tgtccctggt gaatccgagt tcatcggaaa
       61 aggagtctct tattgtgcga cctgcgatgg ttttttcttc aaaggaaaat gtgttgtcgt
      121 ggtgggtgga ggcaacaccg cgttgatgga atctattttt ttaaaaaagc tcggatgtaa
      181 ggaagtcact gttgtacacc gtcgtggtca atttcgcgcg gagaaggtct acgaggagga
      241 agcacgtgag aaaggggtgc attttctctt caataccatc gttgaggaga tccatggaaa
      301 ggaaaacgtc tcatcggtaa gcctttttaa tttagagacg agtgcgcgat cagaattact
      361 ggtggagggc gtgtttgctt ctgttggggt gttacctcaa aacgagatcg caaaggcatt
      421 gcaactaaag acgaacgatg acgggtatgt cgtggtcgat agcgaacaac gtacaagtgt
      481 tcggggtgtg tatgccgctg gtgacatcac cggtggtctg cgtcaggtgg tcaccgcctg
      541 tgcgaaaggt gcggtcgctg cgttgtcatc aacagaggta cttgggaaga aataccctta
      601 ctgaagcttt ggtatctgct cgattttttt cttttgtaga agcttgatca ggtcatcgcc
      661 gctttttttc acaggatcac tgagtgatcc ttgcatcgtt tctggttgaa ttccaatgaa
      721 aataatacag gggacctcat tttccaggta tttgataaaa acggagagtg gtatcccatg
      781 tgtactgagg tgcatcgtcc cgagtttttc tcttgggatg atacggattt caccagggga
      841 aagattcatc tctgcagcat cgatgatgat gagtgttttt ggtttttgtc gtttgatgac
      901 ccctgtgtaa ttctctggga tcgtcccaca gtcaaggacc atatcatgag atgactcttg
      961 tttcaatcgt tcagcgatgt atggcccgat cccatcatcg ccgccctctt gattgccgat
     1021 gcagagtatc agaatgtcct tcatcgtccc ttcaattcaa aatcattttg aataaataat
     1081 cttctgatag gtgaggagaa accacatgat gccggtccct gcggtagata tactactcaa
     1141 cagcatgtcc aaggtgagaa acacataggc catgacagtc agcaggattg ctgtgataag
     1201 atacatggac agcgactggc tttttgcctc cttatggtga taatttcgaa aaatcgcggg
     1261 aatcgcggtg atcatgaacc cgaaattgat gatcgttagg ataatatcct gccattgcca
     1321 catatgtcgc aggggatggt aacagagaat ataaataaat cttttctatg gcgtatcata
     1381 aataaacgat gttcttgggg aaagtatggc gaagaagata acgcttgcac agggtgctgg
     1441 tggtgaactg atggaccagc ttattaaaca acacatcttg cgttttttta acttacaaga
     1501 cgccgcggag gttccactga ccgctctgga tgatgccgcg gttgtcgatg acattgtgtt
     1561 ctccacggat tctcataccg tgcaacccct gattttccct ggtggtgata tcgggtcact
     1621 tgctgtctgt gggaccatta atgatgtttc tgtggttggc gggtctcctc ttgcgttatc
     1681 agcagggttc atcatcgagg agggattctc cttggatacc tttgagacga ttgtcaaaag
     1741 catggggcgt acggcaaaca aggcgcacgt cccgattgtc acgggagaca ccaaggtagt
     1801 cgaaaaaggt gcgttgcagc agttcatgat caacaccagt ggcattggaa aacgaagccc
     1861 tgctctggaa agaaatataa aggaagtcaa aaggtataga aaattcagga gccgctggtt
     1921 acttgacagt aatctttctg cgggtgatac tctgattctc tccgggaatg tcggggaaca
     1981 tggtatcgcg ttgttatcat tccgtgaggg atacggtttt gagacgacca ttcattcaga
     2041 tatcgcaccg ttaaacggct tgatgagcgt tctgcttgct gaaggaggca ttgtgtctgc
     2101 taaggatccg acacggggtg gactagcaaa cacggtcaat gagttttcag aaaaatcaca
     2161 tgtcggactc cttctgtatg aagacgcaat ccccatacca aagggtgttc aatccgcctg
     2221 tgatatgctt ggtatcgatc cgttggagat cggcaatgaa gggaaagttg ttcttggagt
     2281 ggtcaaggaa aaagcagatg atgtgcttgc gatgttgaag aaacatccgt tgggaaaaca
     2341 tgcagctatc atcggtgaag ctacggacaa ggtctccggt gtcgttctgc agacatcggt
     2401 tggtggtaaa cggattttac gtaaaccgct tggtgatcct atcccaagga tttgttgagt
     2461 ttggggagaa ctttttaata gtctcatagt ttacctcagt agcagacaac agatgagggg
     2521 tgaatacggt atcgcaaaag gactagtaaa atcctttaag aaagcgttat gggtctatca
     2581 cattaactca gggtcctgca atggatgtga tattgagatc attgcatcgc tctgcccccg
     2641 gtatgatatt gaacggttcg gtatcaaact tgtcgggtct cctcgtcacg cggatgtgct
     2701 gctcatcacc gggccggttt cacggcagat ggttgacaag gtgaaacgca cctatgagca
     2761 gatgcctgat ccgaaagctg tgatctgtgt cggcaactgc ggcaacactg gggatgtgtt
     2821 ctatgaatca tataatcttg tcgggcctgt tgataaaatc cttcctgttg atgtgtacgt
     2881 gatcggatgt cctcctcggc cggaaaacat tatccatggg ataacaaaag tatggaaaaa
     2941 gcttgagacc ttggagacaa ccgcatgata aagcctgaag atatccttgg ttccttccag
     3001 acgaatttcc caaatagcat caccgaacct cgtatcgaac gtcggaccgg tggcaccctg
     3061 aagactgaaa tcgttcatct gtggtttcat atcgaagcca gatttctcaa agacgcggtg
     3121 aagcatcttt tcacgtttga acagtaccct cattttgcgg tatcttctgg ctatgacctg
     3181 ggggatacca ttgagcttgt gtaccatttc tcgttgtttc atggtgagcg gggacgggag
     3241 atttcattga atatcacggt ttccctgcca aaggaaaatc ctgtgattga caccattacc
     3301 gatcttatcc ctggggcgtt gatctccgag caagaaaaac aagagatgct tggggtaaaa
     3361 atacatggga tccccaagga cgtccgggtg tttgtcgcag atgatttccc aaaggatgtc
     3421 tatccctggc gtcgtgatga aaccggtcca gcgaaactcg tgcggaatct tcatgagggt
     3481 gaatcatgac gactacctca aagaccacgt atcagattcc gattggtccg attcaccctg
     3541 cgttgaagga gcctgtgaac tttacatttg aaatcgatgg tgagaaaatc atcaacgtcg
     3601 atgtacatct tggacatgtg catcggagca ttgaatgggc tgggacgaaa cgaaatttca
     3661 ttcagatatt gtatcttgcg gagcgtatct gtgggatctg ctcctattgt catccgatgt
     3721 ctcttgcttt ggctgtggaa cgtgcagcgc acatacaagt cccagatagg gcagagtatc
     3781 ttcgcgtggt ccatgcagag ttggaacgga tcggctctca tatcctctgg gcgggagtcg
     3841 ctgcccatga gatcgggttt gactccgtgt tgtttttaac gtggcaagtc cgggagacgg
     3901 tcttagatct taccgagtat ctgactggga atcggatcac gaaagggatc acgatgattg
     3961 gtggggtgcg acgcgatatc actactgaaa tggtcccacg tatccatggg acgctgcagg
     4021 atctgaagaa gcagatggag aagctccatc atgtgatgct ggatgattcc acgattcaaa
     4081 tgcgttgtcg gaactgcggg gtgttgacca aagaagacgc attgaagctg tgcgcggtcg
     4141 gaccgaccgc tcgtgcatct ggcgtgcgaa aggatgtgcg tctggatcag ccatatttcg
     4201 cgttcggtga ccttgatttt gagtacacca cccccgatgt gcttaccaac gaggtccatg
     4261 gggatgtata tgatcgtatc atcgtccggt tactcgaggt caaacaatcc attgatctta
     4321 tcgagcaatg tcttgaccgc atgcctgctg gagacattgt cgctgaacca gagatccctg
     4381 ctcttctggc agcgttgaag aaagtgactg gtgaggggat cggacggatg gaggctcccc
     4441 gtggtgaggt ctttcattat gttcgactcg ctggatcaga atccccgtat tcctggaagg
     4501 ttcgggcacc cacgtacaat aatatcctgc cttggattcc tatgttgctg ggtcagcaga
     4561 tcgcagacat cccgattatc gcagcatcca ttgacccctg tatgtcctgt acgaatcgag
     4621 tgaccattgt tcatcacaac cagccaacga tggttgatcg tgaaaaactc catcagttaa
     4681 gcatcgagaa aacaaggagg ctgtgtctat aagatacacg atgattcctc agctgcttag
     4741 ccagctgttc aagaagccat tcacgaatag gcatccgaag aaatatatcc cttcatcttc
     4801 aacgactttt ttcacagatg ttcaaaacgg aaaagaaacg atgaaccccc ctgttgaaac
     4861 cccggagggg ttccgcggga agatcgtcta tgagaaggag aaatgtatcg gttgtaaact
     4921 gtgtctgaag gtctgcccct ctgaggctat tatttttaag gacgaggaga aaaagatcaa
     4981 gatttatctt gctcgctgta ccttttgtgc gcaatgtaat gatatctgcc cagtgaattg
     5041 tcttcatatg agcaacgagt ttctcctagc agatacagaa aagtattcgc cgtccctcat
     5101 cgttgaataa acgagaaata gcttttaata gggtgttggt tgtcggtatc tgtatgggga
     5161 aaaatcggga tatcgcgaag gtccttctgt tgattttgat tgggatgttc gtcccgttcc
     5221 ttggatcgat taccctttcc tatgggtttg tgccaagaac aattattgtc acgtttctgt
     5281 atttccttgt cattttcgga cttgagcttg ggttcgtgta cctgtatttt acttttagcg
     5341 gacgacgagc gaacaagaaa atggaagagt acaaaccaaa ataaatggta cgggtttctt
     5401 gctatacttc tccttttatt cgggttgttt ctcaaaggta aaggataaat aggaaaacta
     5461 gcatcagggg tgcaacgagg taaaaccatt atggtagtaa aagttgataa agcaacgtgt
     5521 accggctgcg cagcctgcgc atcagcatgc cctgttgagg cgatcaaagt cactgagaaa
     5581 gcagaggtca acgaggacac ctgcatcgat tgtggcacct gcgtcgatga atgcccagtg
     5641 caggcactca gtctctaaaa taataaagtt cgtttatttt ttcttctttt tcctttctag
     5701 tttttctcgt acctttggac tcgaaagctc tctgatcgca tctgctgcaa tccagcgtgc
     5761 tgactttgag tttatgttct tgatttcctc tgcggtcttg agtgcttttt tgttcagtga
     5821 aaggtttctt ttcccgatgt tgcgtaacgc ccagttcacc gcttttttta catagttcct
     5881 ctcatccgtc gaatatgtga tacaaagggg gagaaagtct tcgaaatcat ggtctgtcgc
     5941 tgtcttgtca tggaccgtta atcctgcaat gagggagaat gcgccgcgtc tgacaaactc
     6001 tttttctttg tccgcccatt ggaacacttt gtcgtatgca agtgggcaac gatcaaagag
     6061 gtttgtgcat gcttggtcgc agatgtccca tgagtcaaaa tcttctgccc atgcatccat
     6121 ctgttctgag gtgacctgct gtggatcatc gataaagcag gcgagcagtc gtgcgtcatg
     6181 gattttggaa tcccagagtt tgagggcaag agcatggttt ataccgattt gctttgcaag
     6241 gggccgcagg atgtaaatcg atatcccaag gttgttatgc tggttgattc cgtagcgtgt
     6301 cattccttta acgttttctg gatttgctag ggaacggagt tttgacatga tttcgtcgta
     6361 gtccatcgtg gttcacgtga atgcgttatg tctttttatt tcaatatttt tccggtctca
     6421 aggaaccaga ggtataagtc aagttctgcg agtgtcaatt tggtgtttct ggcgattttt
     6481 tgaagatggc cttcgatttt gagatagttc tttttcgtca gttttttcgg ttgtttgatg
     6541 atgcgatatc gtgcgaggat gtcaaggatg tgggtatcga tgattgcgta gtcatcgtat
     6601 ccgatgttgc gcagaaaatg gcttgcttct ttatacccga gtcctttgat gttgtcgact
     6661 agccatctac gtcgttgttc atgtggtaag gtttgaatca ttgtgggaag aatatctttc
     6721 tttttggctg attcgacgat aaaggctgct ctggtgtttg ggaatcgata tccgtgctgt
     6781 ctgagttttt ttgctagggt ttctttggta tcagtgcaga aacattcaga gagttgtgag
     6841 tggatgtgga tggttcgctc tgcgttacag ttggctgtca ggatgcagaa ggtgagttct
     6901 tggaagagtt cctcggcgga atgcgtatca atggttttga actcctgaat tcgtcggtgt
     6961 atttttcttt gtgtgttgct gtgtttgagg tcgttgatga ccgaaagcag atcgttcatg
     7021 gtttctccgt tttagaatgg gtgggtggaa aaaaaaggtg gagggtgtaa aacaaacgag
     7081 gattcttaat tggtgaggag aggaggaaag gatgggatgc aaaaaatatt ttttccccgt
     7141 ttgtttttac atctccacgt atgttaacgt gaggtgtttg gtaccctctt aatttatctc
     7201 caatataccc ctattaacaa ttgttaatac acctactgta tttaaaacta tcgcatccat
     7261 gtcgacgaaa agacaatcca ttaccccctt ctatttttac caatctttaa aaaaaataaa
     7321 aacattaatt ataagtataa attaacaatt gtaccctttg atgaaacgcg ttccctgtga
     7381 atacgtgata tggaatggac ttccccttat cagaaaagaa atcgcagaga gcttgatgac
     7441 caacttcgga ttgagccaaa aacaagctgc agaaaaactc ggtatcacac ccgcagccgt
     7501 ctgccagtac gtctctcaca aacgaggaaa taagacaata gatgatgaag aactaaaaac
     7561 agaaatcaaa ctttccgcac aacgcatcct caactacacg gacgatgcca tgatcaagga
     7621 gacctgccgt atctgcaaga ttttcatggc aaaagggatc tttccttccg gatgtgactc
     7681 ctgcccgcct gagtaaaact taacccggag gaaagaacac ggtaaatagc gagatgacca
     7741 aggggatact gcctaaggaa caaataaaac tcgcgacgag aaactgattc acaatcgact
     7801 cattcccacc aacacgcttc gtgaaaatcg gcagcgcggt gaccggtggc acagcgcttt
     7861 gaagtatcag aagcaacccg atctgatacg acggtctgat gaccaacaaa gctgctaaaa
     7921 agatcaaagg aaacagcaga ttttttatca gaacaaactt cgcaatctct gtactatata
     7981 tctttccttt tcgctggaaa tccagggata gacttccacc gatcaccagc atcaataggg
     8041 gcaccgtcat cgccccaagc atcgtaagga tcgtaagaac catacctggg acatatgact
     8101 ggatgtcagc aaaccggatc atcaacgcaa ggatcgtgga gatgaaaacg atattaaaga
     8161 tacgtctcca atttattttt ctctcgtttt tcccaaaaaa atatggataa atgttgaaca
     8221 ggaaagcagg gtaaaaaatc ataaacaaaa ataaggaaac aagatacgga gacgaagcac
     8281 caaacatccc ggtaagaatc gcaagaggga aaaacaaccc gttatgcaaa aacagactca
     8341 tggcaaactc acgtctcgtc gtcttttttg atgcaaacat aaatgctccg gtcaagagtg
     8401 ctgcaaggcc ggtaaaaaca acccaccaga gcggaagcac ccaccagtct ggtatctctt
     8461 gaggagaaaa atttgagacg atgttcacaa acacaagaca gggcaaagca aggtccaagg
     8521 caagattcga aagggcacca agtgcgtttt ctgcgacaac ccttcgttgg acaatcaaaa
     8581 aaccaaaaac accaatacca agaagaatag ccactgattg gaatgtgata gcgacaagat
     8641 ccatggtata ttcataatct cgaaaacatc caggacttaa agagattacg ataaaggaaa
     8701 ccttaggagt ctggttctga gaagaagcta gatttataaa caaggttctt tatctcaggg
     8761 cgattaatag tacagggagt tatgaatgta tgagaacgac atcgatactt gatctgatga
     8821 cagcagatca tacaaaaatc ctgaaactgt taggcgatgt tgaaaaaagc ataggcattg
     8881 aactggtatc cctgatgaag gtgtttgata cctttgaatg ggagctcgaa aaacatattt
     8941 tcactgaaga aaaagcgatt ttttcctcgt ataacccaag gaacatttct gagggttata
     9001 agatggtccc agagctcatc cagcagcata atgagatcct taatcgcatc cgggtcatgc
     9061 gaaaggactt gatgtggaat aaaccagtgc aatatcccga gttcaaagaa ctgatcatgg
     9121 ctcacaaaac attcgaggaa acatcattgt acccgaaact agaccaagaa ttaaccaatg
     9181 cacaaaaaga tgaaatcata aagaaaatcc gcgaaatcgt ttgagaatat cttttttcac
     9241 gacatcgtaa aaggttcaaa tcgctccgct tttgccttac agataggaca gattggtggg
     9301 ggtatttctc tggcgcacaa atacccgcac acggtgcatc tccagaccct gacagcccct
     9361 gttttttttc ttgctatcgg ttgatctgtt atttcttttg ggacatttgg ttttttttcc
     9421 gctgcagcaa ggaccacatc aataatttcc tgaggtctcc gttccggaat cggtcctaac
     9481 agagatggat tattttttat cttttcattg acaaacaatc cacaataaca catcccgtac
     9541 tcatcgacat cagcgtcgcg gtactcacaa gggcagatga tgtcaacatc gtattctttt
     9601 gcgccacttg cgacacgaca gggacaggac ccgtacccat atcgagcagt atttgttgct
     9661 agaccatcga tgagatcatt gtggagctgt tgatcagggc atatataata cccattgtct
     9721 tttgcgtttt ttttcatcgc ctcaatgata gattcttttc cagtcatgag cggagcttct
     9781 ccataatttc tttatctttg aatccaagaa tcacatgttt tccatgatcg attacgatgg
     9841 tcggataagt acgatacgga ttgtattttt tcacttcatc ggtcgcctct atgtaatcgt
     9901 ttcctgtcaa tgtatcgaca tccacatatt caaaggagat atcaagagct ttgagaagct
     9961 cctttgtttt cttgcaccat ccacaggtgc tcagggtata gactttcacg tgatgtgtcg
    10021 tattcttccc agggattgtt tcgcgtttca ttgcgtttat attctctcgg gctctaaaaa
    10081 ctttatgctt cttcgaaaaa ctagttttaa atagaataaa cctatgtgag acatgaggtg
    10141 aaagcgtgac tgttaattat tatcaatgtc aaacgtgtaa gaaaaagact atcgtaaaaa
    10201 caaagaaaga gaaaccatcc tgctgcggaa aaccaatgaa gaagatatcg gttgatatct
    10261 gccttcagcc agatcatgct gaacattctc gaccgatggg agaagatgaa ccgtgtgatg
    10321 atttccgcgg tggtaattaa aaggagagct gatgtggtat gggaaaatgt gatgtctgta
    10381 gttgtgaatt tgaagaagaa gacatacgac acatagctat aaaaggtaaa ccaaaaaaga
    10441 tttgtcagga atgcgtcgca gcaatcaagg gatttgcgta attacttgtt tttatttgtg
    10501 gaaatatata cggtcctgtg tcgtggtccg tctccttcat aaaaaaagat atgctgccag
    10561 gtcccaagga caagttttcc cttcgcaata agtatggtta ccgatgaacc tagtacgctt
    10621 gccttgatgt gcgcgtcact gtttccttcg gaatgatgat aggatgcgtt ttccggtatc
    10681 aatttttcta atgtttcgat gatgtcttgt tggacgtctg ggtccgcccc ttcgttaatg
    10741 gtgatgccag ccgtcgtatg cgggacataa acgatgaggg acccttcatt gatattttcc
    10801 tctttgacga gtcgttggac ctcatcggtg atatcgatca tctgatttct tctctgcgat
    10861 gagatatgta gttccttcat taagaaggct catctgtttt tttctattta aaccgtgttg
    10921 atgagaaaac ctttttgtac cactgcctgg tacagcagct cctgaagacc atgaagcttg
    10981 ctccaagtgc gatcctcttt gatatggatg gtgtccttgt cgattcactt gattcctggt
    11041 ggaaagcgtt gaacagtgcg ttacagagat tcaaacacca agaaatcacc cgtgaagaat
    11101 tcataatcac ctattggggt catggcctca aagcaaacct cgaacgattg cacctgaacc
    11161 ctgaggtcgc ccgattctgc aatatcacct acggcaacca tctcgatctt gtcacgattt
    11221 accctgatac ggaaaacaca ttgagaaccc tagcgaccta caagaaggtg gtgattacaa
    11281 atactccttc tgattgtgca cggcagattc ttcggaagtt tcatatcgag cagtatttcg
    11341 aggcgatcat caccagtgat gatgtcgtca gatcgaaacc tgacccggag atcgttttca
    11401 aagcatgtga acgactcggt gtctctgtac aaagcgtcct gctgatcggt gatacggaga
    11461 gtgacgtgaa agcagggaaa gcagctggat gtacagtcgt tggtgtgaat atccctgcgg
    11521 atttcatgat tcagaggctt tcagaattac cttctctcct tcgttaaaag agatggtcgt
    11581 tccttttttt cttctcaatc ggaatagtta aaaataacct atgagatgca aggcaggtga
    11641 gtactatgga ggaagatatt caaatcattg aatataagaa cattgatctt tcaaactcgg
    11701 tccttattga agcgttcccc acggttgggc ttgtcagttc catcgctggt catttccttg
    11761 ttgagcaatt gaacctggag gagattggaa caatcagctc tcgttatttc atgcccgctg
    11821 cagtaatcca taacggaatg ccatctcccc ctgtgaggat ttacgcaagc aaaaaaatct
    11881 gtggatcgaa tggtgattgt gatcagattg ttgtgattat ctctgagttc atgccgtcgg
    11941 ttgatatcat caaaccagtc gcaagcatga tattgtcctg ggcgcagaaa aagaaatgca
    12001 gtcatattgt gacgttagaa gggacccatg gcctgaatcc gaaaaaacca aaaacatatg
    12061 gggttgccac gacacagaaa atgaaagaca tcctcaagaa atattccatt gaggaaacaa
    12121 aggaaggcat gattacaggg atcactggag tcttgctctt tgagggagca cggttgaaac
    12181 aggatgtcct gtgtcttctt gctgaagctc attcttcgta tccggattct cgtgcggcag
    12241 ccctgcttgt ggaaacactt gataaaatgc tacctgaaat aaagatagac acccagccgt
    12301 tgtacaagga agcagatgac atcgaacaga agatccggtc tttcatcaaa caagcacagc
    12361 cgaccgcgcc gtcatcggtt cctcttcaac cacagatgta cggataaagg gagcgttatt
    12421 tgttcgtata tcgattcaaa aacacgaatt tctccagggg aaatcgttct ggtcttttct
    12481 tctcaatcgc tgcctgtttt tcagaaagaa taggatagat ggtcgctgca tgcttcccta
    12541 cgtttatcaa cgtgatgacc tcaaggtcct ctgggatacc aaggatctcc ctggtttttt
    12601 tcgggctgta tcctgcgata ggatgtgcca ctaaaccaag ttccgttgct cgtaggatga
    12661 gaaacgcggt cgccatccca gtatcaaatt gatggtacac ccgctcccca attatgcaat
    12721 catattcttt cttgctgagg actacgatga tcattgacgc gcgctgcacc cattcgtttc
    12781 cctttgagat cgcctcatgc attttcttta gcatctctcg ttcataaacg aagacgaatc
    12841 tccagggctg attgttgtta catgatgctg agagttgtgc atgtaatgca aggtccctaa
    12901 ttaaatcctc agtgatttca ataggatcaa gcgaacggta tgctcgccga tttcgaatcg
    12961 cttctttcac atccatgttt tacccccatg acagggaaat tgattgagat taattatccc
    13021 gtggtctctt aaagatttct cttagtcgaa attgaaaaag aaaactataa gatggagtga
    13081 gactatttcc tatctcatgg caatcggatt tgcgttgtta agtataagcc cgcttcatga
    13141 aaaaacggta tatgagaaac tcacccatat atcggaaatc atcgaggtcc atccgctctt
    13201 tggggaattc gatatccttg tgaaaattca agcctctgat atcgactcga tcggaagtat
    13261 cgtgattaac aagattcgat cgattcaagg ggtgatggat tcaaaaacat tgatcgggac
    13321 gaagagctta tccggttaat acctcattct ctctttatac ctgataaagt gctgagaata
    13381 ttttttttac atcccatgta acggtctcgg ttgtccattg atataatcat ggaacaatta
    13441 tatttacaga gatacattta tgtaggagag atgtacatgt cttcgaaagg ggaatatcca
    13501 tgaaacgaag gtatcttaca acatttttac ttgttctgct tcttttatca tcgatatcga
    13561 cagccgttac ccaaaaatgg acaaaagaca cctcgacact gccgaaaaac acaatatatg
    13621 tcgatgacag caacacccaa ggaccatgga atggatcctc cgagtacccc tatcaatcta
    13681 tcagcgacgg catccggcat gcaacagacg gtgataccat ttatgttttt aatgggctat
    13741 attctgagac catcataatc aataaaacca tttctatcag ggggcaagag caagagaaca
    13801 ccatcatcga tggatacaac aatggttctg ttgtaaccgt cgctagcgag gacgtatatc
    13861 tcagaagatt cacgatacgg aactccggcg ggtatcaggg agatgcagga attgctgtcc
    13921 ttgcaaatac caccaccata acggaatgta ccatctacag ggcacgcgcc ggcatatttg
    13981 ttcaaaaaca aagcgaaacc accatcacca acagtcgatt ccatacgaac ggattcggta
    14041 ttgtctcttc atcatccgca tttgtcacta tcgaccaatg taccttctac cataacggcg
    14101 caggcgtcta tctctttaat tccacttgcg tcaccatcgc acactcctat gcagatacca
    14161 acggtatcgg attcctctgt gaacggtcgt cacacatcga catctcagag tcagcagctc
    14221 gtgacaacga cgataatgag ggagggatgt ttttcgtcga tagccagtac atcaacatcc
    14281 tcaactcata tattgtccat aatggtgttg gtgtgaatct cgtcaattca tcagcctgtt
    14341 acattgactc gtgtaatttt tctcttagca cccattactc ctgcaaatta ctaaaatctg
    14401 gatccagtat tctgttgacg aactgtattt ttacaaaaag tcttcgctat ggacttttcg
    14461 ctgaggaaag tgcttttacc atctcttgga gcaacatcta tggaaacgag aactttggcc
    14521 tctatgcaac atcatcagcg attgatgcca ggtacaactg gtggggggcc aaaaccggac
    14581 cagcgcacaa cggtcttgtc agagcggatc gtggaacctg gaacccccgt gagatatctt
    14641 atcgtccgtg gctgacgttt ccgatgcccg agatcggacc agactgggat ctggagaaaa
    14701 cattccagaa accgacctat gccgatccat ggccagagca gattctcttt cctggtcttg
    14761 acacggatgc cgatggagca ccagactggt gggaagtcaa atggggatac tccccaaccg
    14821 tttgggatga tcatcagcat cttgaccctg acaacgactc gctgaacaat attgaagaat
    14881 gctacatgga tcaatttggt tcaaacccgt ttgcaaaaga tttgtttctt gaatttgatt
    14941 ggaccaaaag cattcagggg aacagcacca acaaaccacc ggaggaagaa atcacacaaa
    15001 tgatcgaagc gttcgcccgg cagaacatca cccttcatgt tgacacagga gaacttggcg
    15061 gtggagaaga gctcccatca caaacacatg tctcttatgc tgagatcatc gacatctatt
    15121 ggaattcttt ccttcatact gatttgaata accctcgaca gcacattttc cattatggga
    15181 tcatctgcga ttactccgag agtggtggat ttgcaatcat gggatggaac cacctgaacg
    15241 catttatcat tagtacgcaa ctcttagccg aaaaatattc ttattctacc aaaggctggc
    15301 ttgccacggc tgcgtcgatg catgaggttg gacacacctg tggtctcatc gccactactt
    15361 tcgtagggat tgataatcgt aatacggtca acccgttcta caaagaattc tgggcgtact
    15421 tgccttacaa gagtatgatg aactatcatt acacctattc gttccttgat ttctccgatg
    15481 gttctcatgg acgaggagat ttcaacgact gggcaaacct tgatttctct ttctttaaaa
    15541 acacggattt ttcttaccct gtctaatcag tcttccttcc attttgtcaa aaggacccct
    15601 gcaatgaaga agatcgcagc aaagataaca acgatgccac cattgatgag cgcttctgtt
    15661 tgattcatat aaatcatggc attgcgtaat ccttcgttga cataatacaa tggcagtgca
    15721 tgagcgatta cctggatgat cgaaggcatc gcatccacag caaaaaaagt gccagccagg
    15781 aacatcatgg gaaaactgat tgcccccgca gccatatctg ctgtttcttc ttctttgacg
    15841 aaccgaccga tgatcatccc gatacctgag aacagaaaac tcgtggcaat aatgataatg
    15901 atggtaagca ggttgatgtt cactttgatt ccaaacgcga ggattccaac gccgattgca
    15961 accgaagttg agataaacgc aagaaacagc atgaatagca ttttggcaag tatccattct
    16021 gacctggtga tcggcgtggt caacagcttt cggaggatac cgtcttttct gaatttcgtg
    16081 ttccgttcaa tactcccata aatgctctgc tgcataatgg taaaaccgat catccctggg
    16141 ataaaaaaat caatgaaccc gaaattttct gtaacggtgc tttgttcatc taatccaaca
    16201 acggttctcc cattggagag ctgcatgttc aacccttgca acacactgga aatcacacta
    16261 cgtaacacct gattggtttg ctgttctgtt ggatcaaaat aataggtgag attgactttt
    16321 atcgaaggat cagcaaacga acgggtgatg ctcgtttcgt atccttgtgg gatgacgatg
    16381 agatttttga tgttgttctc cttgacataa tcggcggtcg tctcattcgt cgtaacgttt
    16441 ttaatagtga ggacacctgt tttgtttagg atggtaacga gactctgtga catgcccgtg
    16501 ttatcaagat cctggacgta taacgtgtat ttgctttcgc ccattcctga gaagatcgct
    16561 ccaaagagaa gaattagcat aaccgggaac aacagggacc aaaacacggt gcctctgctg
    16621 cggaaccagc tgcggatgct aaagagaaaa tcaatcccga ctatttttag attcatgttt
    16681 ctacctcctc gatgagtcgt gctccggtca gctggaggaa cacctcttca aggttagagc
    16741 cacggatatc taatccatcg tactcaatgc attcatgacg aagctctgag agaacctcaa
    16801 ggaccttttc cttatagtca attttgatag aaagatttcc gtttccgctt gagtggactt
    16861 caaatccttt ctcttccaac agctgtcgcg cctcgtctga atggcagttt tttatgtaaa
    16921 gggtcgtttt ttcaccgtat ctgttaagga gatcttgcaa tgttccttct gcgatgattt
    16981 ttcctttatg gatgattgcg atatggtcag ctagatgttc tgcttcctcc atgtaatgag
    17041 tggtgaggaa cacggttttc cccttgttcc gtagattggc gatcacctgc cagacttccc
    17101 ggcgagcctt tgggtctaac cctgttgtcg gctcatcaag aaagacgatc tcggggtcgt
    17161 tgactagtga taaggcgacg ccgacccgtt gtttcagtcc tcctgagagg tttctgtaca
    17221 attttttttc atgggaacgt aaatccattg catccagaat ctcatcgatg tttgctcgtt
    17281 tcttatatag ttttgagaaa tacgtgagtg tctcacgtac ggtgaatcgt tcgaaggaat
    17341 gaaactcctg aggtaagatg ctgatgcgtt ctttgatttg gttgaaccct gtttttgtat
    17401 cgacgccaag cagtttgatg gctccggttg tcggcgttcg tatcgattct atcatttcca
    17461 cggtggttgt ctttcctgct ccgttcggtc caaggaatgc aaaaatctct ccttttttga
    17521 cggcaaagga gatgtcattg accgcgatta ggtcatcata gacttttttc agatgagaca
    17581 cttcaatggc aacggtattg tcttgtttat ccccgaggat ggtgagccgt cctttcatat
    17641 ggcaggctgc acaggtcacc acttttgttt ctccagggtc gccgtagaag gtggcgattt
    17701 ccccacagtt cgggcatttg aaccgttgct ctgtcatgtc cttcctcttc tgatatttta
    17761 tgtgatacct gtgattaaaa ccatgtatat aggttttatc ctattggagg aggatctttt
    17821 tttgttgttt catctgagaa tcaaaggtat atacaatggg taccccggta gggatctcga
    17881 tttttgggat ctcctcatcg gagatgtttt cgacgtactt tgtaatcgat cggaggctgt
    17941 tgccatgagc gacgataagc acgtttttac ctgcttggag ttccggtata atattttttt
    18001 tataatatgg aagggtgcgt tcacaagtat ctttgaggct ttccccgtgg ggtgggttga
    18061 tgtcgtagct gcgtcgccag atatggacct gggcttctcc gtatttcttc atggtctcag
    18121 ccttgttaag tccctgaaga tcaccatagt atcgttccgc taaatccacg gattgataga
    18181 taggaatctc cttgtccctt gtaccagtat aatgctccca tgcatggatt cgttcttctt
    18241 catgatacag gatcgggatt cgttcatctg agagttcaag cagaatataa tgcagcgtct
    18301 ggatcgccct catgagatgg gagacgtaca tcgtgtcaaa tcgtatgtct ttgagtttct
    18361 ttccagcaga ggaggcttcc tctcttcctt ttggtgagag tgggacatca acccagccgg
    18421 tgaatctgtt ctctaaattc cattgtgatt gcccatgtct gaccaatacc aaggttgcca
    18481 acgtcaacac ctccttatac tccgtagagt ggataatatt tctcttttag atatccaatg
    18541 aagactttag aatttaaccc ttcgccgcag gttttctgaa tgatctcatc agcggtcatc
    18601 aacgaaccat attgatatac atgttcggag agccattgta agattgttgt gaaatcacct
    18661 cgtgagattt cctcttgaac ggtctgatgt tctttttgga attgtctgaa gagctgtgat
    18721 gcgtagatag tcccaatcgc ataggtgggg aaatacccga aatctccccc gctccagtgc
    18781 atatcctgca ggacaccttc ccggtcgttt tttggggcga tacctaagaa ctcggtgact
    18841 ttctgattcc agcatgctgg taactctgcc accgtgattt tatcctccag gagatcgagt
    18901 tccagttcga aacggaggat cacatgaaga caatatgtca actcgtcagc ctctgttctt
    18961 atcaaggttg gtctgacttg attgactgct tgataccaag taacggtatt catggtttta
    19021 cattgatttg gagcaatctt ctggaatatc ggtgaaaaat aagtccaaaa tggtttgctt
    19081 cgtgctatca tattttccca gaacctggat tgtgattcat ggatacctag agatggggcg
    19141 tctgagatga ccgtgtcttt gtattcacct ttcaggattc cgagctcgta gagcgcatga
    19201 cctgcctcat gcgctgttga gaaaaaagac gataggaaac cttcacgttc gtagttggtc
    19261 gtaaatctca catcatcatc tcccatcgag gtggtgaaag gatgggtgga cacatcaaga
    19321 cgtgtccggt caaacgggag gttcatgtgg gtcagcagga tgctgcagag ctttttttga
    19381 caattacttt ccattattag ttttacaggt ttttgttttt gatacatggc acttgaggtg
    19441 atggtctgta agagttctac aagttgttgg cgtaaaatcg tgaattcttt ttgtaggaca
    19501 tcaacggtca taccttcttc gtaatcatcc agcaaggtgt tgtacgggtg ccctgggagt
    19561 ttgacgtaat catagtattg tttttcgagc tcgacgattt tttcaagatg tggttgaaac
    19621 aaagagaact tgtttttttc tcgtgcctcc tgccagacgc cgtatgctat cgatgtggtt
    19681 tttgtcattt tttcgacaaa ttctgctggg agctttcgag ctttttcgac atcgcgatat
    19741 aatttggtga cgaccaaatg atcttttttt gatagcgtgt ttgcaacgtc atcgagtttc
    19801 ttcaaatggg tatagagtgt ttctgaggtt actcgttcat gggccaatcg tgagatgagc
    19861 gagagctggt ctgctcgttc ttcacttcct ttcggtggca tgtaggtcat ctggtcccat
    19921 cccagcagtg cagcaatacc accatagagg gatacttctt tttgttcctt gtagatataa
    19981 tccagtgatt ccttcatagc tttctctcct tcttcatacg gtgatttcga tgccaacagg
    20041 gcagtgatcc gatcccatga cctcttggct gatgaacgcg ttttttatct ttgggagaaa
    20101 ctccttgttg acgaagaaat agtcgattct ccagccgacg ttgcgtgtcc gtgcagcggt
    20161 tttcaggtcc caccaggagt aatggtttgg ctctggatgg aatcgtcgga atgtgtcggt
    20221 atagccatgg tcgatgaacg tatcaatcca cgcccgctcc actggtaaga accctgagat
    20281 gtgctcgttt tcctttggtc gtgcaagatc gatttcttta tgcgcggtgt tgaggtcacc
    20341 gcagacgatg atgttttgtt tttgtgcttt gaggttatct gcgtagctga ggaactcgtc
    20401 atagaaatct agtttgtatt ggagtcgttc tttgtttttc ttgccgtttg ggaaatagat
    20461 attgaacaag gtaaaggaag gaaacgtggt gataaggatg cgtccttcgg tgtcgaactc
    20521 ttctctgccg aaaccgtttt tgactgataa tggtttttgt tttgaaaatg ttgcgacacc
    20581 gctgtagcct tttttttctg ctgggttcca gaatgtataa taccctggga cgtttctgag
    20641 atggggtggg agctgctccg gtgttgcttt tatctcctgg agacacatga tgtctggtga
    20701 ttctttcaga aaccaggtga aaaacccttt tttgtctatc gctctgatgc cattgacgtt
    20761 ccagcagagt agtttcatca aagagtacct cctttgtttt tcttaaggaa ttactaggta
    20821 tttctttttc cctttatatg gttttgaata cgatgctatg aggtacgttg gagaagagcg
    20881 atgtagaatc catcggttcg atcggtgtcc ggccaggttc gtttttcatc caataggcga
    20941 aattcactat ggttcttgag aaatcgttgg atctgctctt ctccttctga aggtaggatg
    21001 ctgcagacgg aatagaccat gttgccgtgg ggtttgagta gtgtacaata tttttcgaga
    21061 aggtcttgct gtaggtctct cagtcgttcg agatcctttg ctgtcagttt ccatctgatg
    21121 tcaggatttc tctgtagcgt ccctagtccg gaacagggga catcgagaag cagtcgatct
    21181 acggtttctt ttatcctttt atagattttc gaggtggtga tgagtcgtgt ctcaatggtg
    21241 tccacccctg cctttgccgc tcgttttcgt agctcccgta gtctccattc ctgggtgtcc
    21301 atggcgatga tcttcccttt gttcttcagt aacgctgcga gatgtaatgt tttgcttcct
    21361 tcccccgcgc aggcgtcaac gatgcgcatc cctggttgtg ggtcaaggat ccggctgacc
    21421 atctgcgaag cagggtcctg gacctcaaag agccctgcgt tgaaactgtg aagtttgaaa
    21481 acatttattt tctctttaat gaacagcgcg tcagggttcc catcgattgg ttcagcagcg
    21541 attccttctt tcctgagaat caccagcaat tcctttggcg atgttttcag ggtgtttgct
    21601 ctgatgacaa gctcaggttt tttatttaac gagacaagca ctggttccca tcgcatgccc
    21661 agttcttttt ccccttgagc atccagccaa tccgggactg attctcgcag ggctcttgaa
    21721 atctttgtgg tttttattcg ttgtaagagc tgcgttgcgt cgaggtcttt tttcttttga
    21781 agagcggaga gatctccttt ttttgagaac aggtagatgg tgatgagatt ctggagctct
    21841 ttctcatcag ctgatggctc tgtatcgatg atgtaccaca gcggtctcca ccatcggatg
    21901 agatcataga tcgtatcgga aaagactgct cgtttgctct cctcccattc tggatggagc
    21961 ttcaaggtat gttcgatcgc cttggtcgca aagacgttct gccaaaaaac tgcccgtaat
    22021 gtttcaatca tcgctggggt gagttcttga agggtcgttc gtagagttgt cgacatgggt
    22081 gttttcttcc taggatatca tctgtcatcg atacttgatt aataaattca ttcgctttct
    22141 cgcaggtttc tttttgatac aatttggatg aatactttca ctcaccccta ctcgagattt
    22201 ataaccatga acgaccattc catgattaat tgactttgag ttacggagga catgccagcc
    22261 aggaatgctg gcatttctgc cactttccct ttgcatctgc atcccatccc tctctccttt
    22321 ttttcttttt catccaaaag ggttttatat ataaaactca taaagaaatg tgatcacaac
    22381 cggaaaatgg aagtggttta ggaggagtac atatttcaga aaaaatgtgt gagatgtgta
    22441 agcaaaaatt ccatctccag gagcatcaaa gtggtttggt ctttgatgat aaatttttca
    22501 tctgtgagga ctgccgcacg aacacaccag aacaggagat actaggatgg tcccaaagca
    22561 ccatgcgttc atctgcttca gcgatgccga tctccctgtg gctcatccaa gagcagaata
    22621 aggacaagcc accgttctca agaagaaaag aataactcat ctactgtgat gttctggcca
    22681 tcagtaatct atcaaaatcc ttttttatag ccagtattat gcgggtagcg tatgtttatc
    22741 atcaaactcg gcgggagtgt tattaccaat aaagcaaagg aatgctgctt caaacaaggc
    22801 atcatggacc agcttgctgc tgaaataaaa catgcgaaca aacaggtcat cttagtccat
    22861 ggtgcaggat cgttcggtca tatccttgcg aaacaatata agttaaatga cgggttcaga
    22921 caaaaaaaac aggttgaagg attcgctctc acgcaagcga tggtccaacg gttaaacaat
    22981 ctggttctcg catcgttgca taagcatgat atccctgcag tatccatccc accacatgca
    23041 atcctctccc tatcagatca taacctcgca cgggtcgatt acaacatatt caaaaaatac
    23101 cttgccttag ggttcatgcc cgtcagtttt ggtgatgtcg cccttgataa ccaacttggc
    23161 ttttccatct gttcggggga cctactcatc caactgctcg cagcagagtt taaaccagaa
    23221 aaagtgattt tcgtcattga cgaagacgga ttatactcag ccaatccaaa gaccaaccca
    23281 aacgcgatct tctttgataa caccacggta aaagagctgg aaaaactcac cacatcgtta
    23341 gatgcacatg cagatgtgac caaaggaatg aaaggaaaaa tacacaccat caaacagata
    23401 gcaaaaacag gggtggacac tatcgtattg aatgggaata tacataaccg attatatgat
    23461 accttaaagg ggaagaaggt acgatccacc atcatatacg gagataaaat atgaatcaaa
    23521 cggaggaacg gaaacgcgaa catgtgcaga tatcattaaa agaaagcgtg tctgctcatt
    23581 ataattactg ggacgacgtc catctgatgc acaacgcact cccggagatc aatgaaaacg
    23641 atatcgatct ttcgacccag ctgtttgaga agaaactcag agccccactc atcatctcag
    23701 gcatgaccgg tgggtatccc gaaggaaaga agatcaacga acatctcgca gcagccgcat
    23761 ccaccttcca gataggcatg ggggtcggct cccaacgtgc tgcccttgag aaccagaaac
    23821 ttgcggaaac ctacagcatc atcaaggaat atgacatccc attgaagatc gcaaacatcg
    23881 gcgcatccca gatggtcctc tgggatagac aacagatgct tgagaacgca ggaaagatcg
    23941 tcgagatgat cgatgcagac gtgctcgccg tctgtctgaa tttcttacaa gaggcgatac
    24001 agccggaagg cgaagcacag gcgaaaggat gcctcgcgac catagatatg ctgtcccgag
    24061 agtttagcct cccgatcatc atcaaggaaa gtggcgctgg tatctcaggc aaaatcgcgc
    24121 accagctgaa aaagacgaag atccgtggca ttgacgtcgg aggcgctggc ggcacgtcgt
    24181 tctctgcagt ggaatattac cgcgcaaaga tgaaaggcga cacccatgac atgcgtgcag
    24241 ggcaaacatt ctgggactgg ggtatcccga cgccaacatg tgtccttgag gtgggaaaag
    24301 caaccagctg ggagctgccc atcattgcga ccggtggaat ccgccatgga ctggatatcg
    24361 caaaagccct ggttcttggc gcaagcgcag caggcatggc acacgccctc ctcaaaccag
    24421 cattgaagga taaaaaatcc cttgagcagg agcttgatgt catcactcga gaactccgca
    24481 tcgcgatgtt ccttgtcggt gtcgatacag tcgaaaagct atcaacggtg gaagtaagat
    24541 atggatctac agactgaact tgtaaaacga gcggcgatat ttaacaccta ttggcaacag
    24601 tacctgcaga taaaaaaacc aacgaagcta tacgaggcgt cccaacatct ccccgctgct
    24661 ggtgggaaac ggatcagacc attcctcacc atgacctcct gcgaaagtgt ttcaggggac
    24721 atactgaaag cactcccact tgccactgga ctagaattga tacataattt tacattagtc
    24781 catgacgata tcatggatca ttccttgcta cggcgcaacc ttcctgcggt gcacgtcaag
    24841 ttcggagaac ccgctgcaat cctggcgggg gatttgctat tcgcgaaggc ttttgaggca
    24901 ctccttaata catctgttga tttttcgacc tataaactcc tgcagcagga tttcattgac
    24961 ggtatcatag cgatctgtga aggacaacaa atggatatgg agtttgaaag acgcaaaaca
    25021 gtcacagagc aggaatatct gaatatgatt ggaaagaaaa ccggcgcgtt atttgagttg
    25081 gccgggaaag gtggtgggtt gattggtggt gggaacccat cagaaatcgc tgccttaaag
    25141 acctatggga tgacgctggg tttagcgttt cagatttggg atgattacct tgacatgagc
    25201 agcacggcaa caacactggg aaaagacata ggcaacgata tcagaaacgg aaaaaagacg
    25261 ctcatcgcgg ttcatagtct tatgcatgcg accgggaaac acaagaaact tcttgaggat
    25321 gtgttcggaa accatgcggc atcggaacat gatgtcacac tcgtctacga ccttttcaaa
    25381 gaactcgggt ctatcgagta cgcacaacgc cgtgccttac agatggtcaa ccaggcaaaa
    25441 gatgcgataa aacccgtaaa atcatctgat gccaaagaac tcctctatca actgatcgag
    25501 tataccatcc agcgagaaaa gtgactatgt ctgctcagag gatacagcgt gaggcgcgca
    25561 agttcgcgtt acagaatgcg gtgtcattca acagcaaggc gaacgaaaaa gctgtcgtgg
    25621 ggaaggtcat cgctgcatta aaaaaagacg gtgtgacccc cgcagagatt ataccaatcg
    25681 tctctcaagt ggtggcacaa gtcaatacca tgtccgctgc tgatcagctc gctgagctca
    25741 ccacgcttgc acctgagttg ttacaaaaag aaaagaaaga aagagatttc tctctgcccc
    25801 cgttaccatt tgctgaaaaa ggaaaggtcg tgactcgttt tccaccagag ccaaacgggt
    25861 atctgcatat cggtcatgcg aaggcagcca tcatagactc tgaatacgcc cgactctacg
    25921 atgggaagtt cattttacga tttgatgaca ccaatccaga gaacgcactc ctcgaatttt
    25981 atgacgcgca aaaagaagac ctacactggt tggggataca atgggatgtc gaataccata
    26041 cgtctgatca tcttcagacg cattacaaac ttgctgaaca gctcatcagt caaagggatg
    26101 catacatctg caaatgcacc cctgaggtga tcagagaagg caggtaccat gcaaaggctt
    26161 gcacgtgccg agaaaagctc gccggacctg ttggcctgga tctgtggaag cagatgcttg
    26221 catcccgtga gacaaatttg attcttcgat tgcatgggga gatggacagc gagaacaccg
    26281 caatgcggga cccaaccttg ttccgagtta tcaacacaga acacccattg actggcgata
    26341 cataccatgt ctggccaacc tatgatttcg ctggcgctgt ggaggatagc atcagcggag
    26401 tgacccatcc cttccgaacc aaggagtacg agttacgaga ccaggtgtat ttccgaattc
    26461 taagcctgtt gaacctgcgg gcgccccatc tgatggagtt ctcccggtta tcgatcgacg
    26521 ggatgccggt ttccaaaaga aagataaaac ccctcatcga acagggactg gtctccgggt
    26581 ttgatgacgt caggttaccg acgttgcggg ggttgaagcg tcgggggatt gttcccgagg
    26641 cgattaagca atttgtgctc cagcagggtt tttcaaaggt cgagtcggtc gtcgatttca
    26701 gtttggtcga atcggtgaac cggaagtatc ttgactcccg ggtgaagcgt tattacttcg
    26761 tccctgaccc cgtacaactt gtcgtgaatg aggctccgaa aaaaaaggta aaactctctt
    26821 tgcatcctga tgcgaccatg ggtgatcgga ctatccatac cgcttcgact ttttttatcc
    26881 cacgacaaga tgtccaggtg atgaagatag gcgatgtgtt tcgactgaag ggattatata
    26941 acgttaaaat cacaaaaatc ggtgagacgc ttcatggtga ctatggggga gaagaactca
    27001 ttcctgagac tgcgaagatt caatggacca ccgatgaatg tatcccattg aaggtatttg
    27061 tccctgggct gatcttcaaa ggggatatct tcaacccgga tagtctccat gagcttcaag
    27121 gatgtgccga gaaagcggtc gctgatgtgc ctgctggtga gatcgtgcaa tttgagcgtg
    27181 tcggttttgt ccggctagaa aagcagaaga caggatgtgt gggttttttc gctcataaat
    27241 aatttttctc ttttgtatta acgagttccc gaaatctctt tctcaacctc agtataatcg
    27301 tagtaattct cactttgaag gaaggtattt aaacatcaat gtgatttctt ctccctgcag
    27361 ggatgcagat aaaaaaagga tgggaatcga tgcgtttcat gagtatagta gtaattatgc
    27421 tagcatgttt tttccttggg tcaacggtcg tatgtcgcgc tgcccccgaa ctccaaccag
    27481 acatcgatat gaccacgatc gcggtaatca tcacggtcat cgccttactg cttgcgtttt
    27541 tcttcctcat gggcggtgtc aaatacgtca cccctgagaa cgtccttgac actgagattc
    27601 gaaaaaacct ctattcgtac atcgatgagt acccaggaag tcatcttcgt gagatcgcca
    27661 gagagcttga tctcaagccg agcaatgccg cctggcatct acggaaactt gagcagacca
    27721 atctcatcag aagtagagct attggtggaa aaaaggttta ttaccttgtt gaaggaggaa
    27781 tcgaggcaaa acagcgcgcg atttcagagt caatcatgcg gaataaaaac gctcgagaca
    27841 tcatggcgta cctttcagag catccaggca agcacctcct cgagatcgcc catgcacttg
    27901 ccatcaacca tcatgtggtg aaatggcata tcaataagct ctatgaggct gagctcatcg
    27961 aaggagacac cacgaactcc gcgtacccaa tctattatcc cacagaaata ggacggcagt
    28021 tcatggagac gtataccgag tttctgaaaa caacggtcga gaacgctgct tagattctgc
    28081 cgacgtcaac gacttcgacg ttttcgactt tttttatctt cccgaacttt tcgacaatag
    28141 gatcaagacc gccctcgaca tcagggactg cgattgtcac atcaagacct cgaagaccaa
    28201 acgcgatttg tttgacttcg acgcggccga gtcgagctgg tttttggacg acttttttta
    28261 cttcttcggt gattaaatcc atatccttgt cggaaatgac tccatctggc atgagacgaa
    28321 taagtgctat gacttccccc atcgggttat cctcctatgg tcctctgaat ccacatttcg
    28381 ggcagagata gtcaacgctt tgttctcggc agtttgcaca acgtccaatc atggtgtcgc
    28441 aggttgggca ttggaatgtt gtcgagcctt gttcaagcaa gccttttccg catgaaatgc
    28501 atcgatcagc ttttttcata gggaaaaccg tatatccttc tcatttttaa acctgacgcc
    28561 ctggtctttc ctggaggatt acccttcttt tttctcgatg gtttccaatg aaaacgaggt
    28621 gatcgattta aaaatccggt actgaaagac cgccccacat atgacgttca tatgatacga
    28681 gatgaatcgg aataataaca caaacacccc gagaagatac tgatgtgtcg caccgagcag
    28741 aacaccgtat aacgcagcca tccccagttc ggtgacccct gagcttcctg gtgtcgtcgg
    28801 aagcattaca atgatcaaca acaaggcctg tgcggcatac gactcgataa aaaacggggg
    28861 cagacccaat gcgagaagga tcatcgatgg gatcataaaa ccagtggacc acattaggac
    28921 tgttaacccc ccggcagtaa gaaacgattt tctcccttta gttaaaaaga gcaccatgct
    28981 ggtatgaaag ttatcaatct catgttcaat tcgatgtacc acctttttac ttcgctctgg
    29041 ttttttcgag aaccgactca gacgattgct gaatcgaagt aacaacgcct tggttttttc
    29101 cgggtatttc aatgctaacg caaataaaat gatacctgac ataaatatga tgataccgat
    29161 aatcaatcca taactgatca gcttgacatc aatcctttct ttaaaaacaa aaaaaccaaa
    29221 cgggacaatc actaaaataa aaatcgcatc aatgagtcgt tctcccagaa cagcagctgt
    29281 agccccgcca gtagttaatc catccttatt caacaaataa atgcgcacag gctccccccc
    29341 agcgaccgag ggagtaatcc ctgcgagaaa ctggtttgca atcacaatct ttgttgcttc
    29401 ccacagactg atatggacct tcgaatcaat tgctttgctg agaaccttta atcgtgctcc
    29461 ccagataaac cagtacaaca cattcaatgc gattgcgatg ctgaagaata aaaagaattc
    29521 gccgcgaaac gacacctgcg atagatattg gattgtttgt gcatcgatgg taaaatacaa
    29581 gataaacaca atgattgaaa gactcagggc gatacttacg aaaatagaga tttttgtttt
    29641 gttccatttc tttttctcgt ctgtcaataa agagggttat tttattgacg ctataagtct
    29701 cttgtggtag attcgagagt aggaaaataa aaaaccttat gtcctcccac caattacgcc
    29761 tcgtactatt gaagcattat gaaaaaatta ccggtaaaaa aataccttca aaccatgcgg
    29821 tacgcagcga cgaactatct cgcaaaagaa tgtatctacc ctttttatgc ctcttttaag
    29881 gtcacccatg tctgcagcct gaaatgtgac ttctgcaacg tgtggcggga aaagacatcg
    29941 gacctttcaa aagaagacgt gttcaaggtt atcgataata tcgcaaactc aagcatcatc
    30001 gtcctgagtc ttgagggtgg ggacccgctc gtacgaaagg atcttgggga aatcctgcag
    30061 tatgcccatc agaaaccatt ctacttgttt tttaccacta acggtcatct tcttgacaaa
    30121 cgaccgatga aagagtacgg gaaacacatc gattatctgc atatcagcat cgatgagggg
    30181 catggaaacc ttgcgttctt tgaccgtctg gaggagttcc aaagctatgg tccggagatc
    30241 tgcatccaaa tcgtcgtcac cagagaaacc ctcccggcac ttgaagagaa agtcaaacgg
    30301 gtgtatgagg tgaacgcccg tacggtcgtg atgccagctt gtcatctgga tgggactgat
    30361 gatttctatc ctgatccgaa acagttccaa ggagaaatac tccgcttgaa acagaaatac
    30421 ccaaatacga tcaccacacc gaaaggtttt ttagataaca tcaacagacc acatggttgc
    30481 tccacctcct cagtgatcat cgactcggat ggcggtctgt tctatccgtg tcggaccgtg
    30541 ggaaaacacc tttacaattt cacggaaggc tcttttatgg agttcctacg tactccagag
    30601 gcgaaacaag caaggactgc gatggatcaa tgtgaccgca gctgtggatg gtaccagtat
    30661 ttcgccactg acgtgttcgc atcaccccgc tctctgtatt cctccattag tccgtacatc
    30721 ctcaaatgat catcctatta ttttttttga taactcttat gtagtcatcg ctggttaaca
    30781 acaacaggac ccgactctta tgaagatact cttcaccacg gaatcatact atccgattat
    30841 tgatggtggc gcgatcgcac agcaccgtat cgtccatgaa ctcatcaaga aaggccatga
    30901 tgtccgggtg atcgccccgg ctttctcctt taaaaacatt gtggagaacg agaacggatc
    30961 gacaatctac agaccacgcg cgttcattct cccattttat atgaacaata agtaccattt
    31021 cgcccccttt ccatttctgt atgtgaaaaa aatcattgag ggattcaaac cagatgtcat
    31081 caacgtctgc tccccatatc cgataagcat ctgtgctatg atcttggcaa aaaagaaaca
    31141 tatcccgttg gtcggctcga tccatatcct accggaaaat gttcttgccc ccttcttttc
    31201 ttcttcttgg tatcctaccc tggtgaaata catctggtcc tatctcatct atttctacaa
    31261 tcgtgttgac tgggcgacgg ttccgacgca gactggtgcg gtcatgtacc aggagaaagg
    31321 attgaagaca ccggttagtc cgatctccaa tggtgtcgat acagagatgt tcaaaccaac
    31381 caacaagggt gaatatctgc gaaaaagatt taatctccct gaaaagccac tcatccttta
    31441 tacagggaga atcaaccagg agaaaaacgt tgacgtgctt gtaaaagcga tcccttttgt
    31501 tctcaaaaac gtcgatgcac attttctttt ctgcgggtca ggaggtctga aaccagacat
    31561 gatgaaattg acgcaacagt taggtgtgga tgggcacacc acgtttatcg atttcctgga
    31621 ttgggctgat tacccgaaca tttttacgct tgctgacgtc tttgtcatgc cagcggaatc
    31681 agaactccaa agcatcgtca cactcgaggc gattgctacc ggtgtaccac cagttgtggt
    31741 caataaagga gcggtgcctg agcttgcgag tgcacagaac gggttggttt ttgaaccaca
    31801 agacagcaat caacttgcca aaaacatcat tactatcctt tccgataagc aactgaggga
    31861 caccatgaaa aaaaacagtc tgaaactgag tgaacagcac tccatggata tcgttggaac
    31921 ccagtacgaa caggtctatg aaaaagtcct caatctccca agggagagtt aagggtctgt
    31981 ttttttcacc atgagatgtt gttcgataaa cgtccagttg acgctgtaga cttcagccca
    32041 atgaatttct tcgttatcct ggttgaccgt ggcgttccgg tagacgagct caaacggttc
    32101 ctgtgaaaac ttcagaaacg actcgttggt catatacacg ctctgcagga cacggacgtt
    32161 gaccgagata tcgcgcatca catcatcgat gagcacatgc gtcaccacac ggtggctctc
    32221 cccatcgaaa tcagtgatgc agcctaccca ggtatcacac gtccaggtat cattagtacc
    32281 ttcgaaggtc gcgtcgatgc cttctgccca tatcattttg gatagccgta ggtcggtggc
    32341 aaccagggaa tcattcctat caaggttgtc gtggatccat tggatagcat tggcagtctc
    32401 attcgggatg ctttcatcca tccactctag tgacgtgtac acggggtaga ccgctacggc
    32461 gtttgaaaac accaggccgc agacgaccag gacaaacagc gcctgaatcg tggggtatga
    32521 gagctgtttt ttgagaatca gcgacacttt atgctccctg gatctgaaga agtacaagac
    32581 accgacggca gcgaacaaac acgcagggac cgtaagatat tcgacatgac gatctgggaa
    32641 caatgtcgta tttaccgtta ccagtgaata gatgaatgat gaaaggatag cgcataacca
    32701 ggcttggaag aaccagtggt tcttcacatg tttcaggtat tcaacaccaa ggcatccgaa
    32761 cccgatgaag agcagcatcg ggattgaata tagg
//
LOCUS       DATMXJ010000013         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_57667, whole genome shotgun sequence.
ACCESSION   DATMXJ010000013 DATMXJ010000000
VERSION     DATMXJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57667"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..582
                     /locus_tag="VN377_00495"
     tRNA            512..582
                     /locus_tag="VN377_00495"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:544..546,aa:Cys,seq:gca)
     gene            614..3082
                     /locus_tag="VN377_00500"
     CDS             614..3082
                     /locus_tag="VN377_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWR26895.1"
                     /translation="MIRKKTFVDMVIFYCVFSGLVLPVIPEPVKSASGETLLINEVMF
                     HPIEDENTNEWIELYNPTTEALDVEGWMIADEKETDIILADTVNGDGSTSIPPGCYAI
                     LTDKETTIYETYIILDNAVRLSVDDSTLCGYGLNNKQEKIILSDSDGDLVDAIEWGED
                     YDDIPGSPFVFVSEGNTLARYQGTDTDDTSVDFFECSSPSPGSENIQTMREEQPEVTA
                     SEEEESEETTGDVPPQILITELYYDAHPTINAEYIRLFNPMNKSIDVSGWYLTDKPWK
                     EPDDQSKILFPEHTFMLPQTSWFISKNASAFFWETAMLPDFEYGVDSQLAVPQLLTFR
                     TVTFSNTGGLVGLYTAAQSLVDLIIYGETDEYVSGWEGPSIPPSGQGVILKRNSINGT
                     VVDTDSASDWMHHRIYQIGQSDLPLQTIASTGEITTFVSPDNSYDTITRELRDAQYSI
                     DMNMYEFTNPFLYQELLDALKRNVTVRIFMEGSPIGGIDDREGFILGDLAAHGGLIRF
                     IVSDDENHVNARYQFDHAKYLLIDNETVIVESCNWAKTGVPKNPTYGNREWGIVVRNK
                     EVATGFWQVFQDDWDPLHTDSCPIEAMNLTFYPDFTLDYEVPTGSYLPQFDAKTITGP
                     CTITPVFSPDTSEQVLLDAIDAATSTIYVQQLYIYRDWGETTSPLVEHLVNKSLEGVD
                     VQVILDYQTDYEETITKLDETKEFLETYGVRVKFISSEWSPFSTIHNKGMIIDNTTVL
                     ISSINWNEQSVRKNREAGLLVNSEEVATYYATVFLSDWNLEASKTDTSFFSWADYKYL
                     VLIAVVVCITLVFIARDWRKRKWT"
     gene            3073..4023
                     /locus_tag="VN377_00505"
     CDS             3073..4023
                     /locus_tag="VN377_00505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWR26896.1"
                     /translation="MDIAFLQPSWISLIAIGIMVGTLVIAFLKKITLTYSIIIACFFV
                     FLISLFFRTEIINDLGFRPSYLSLEAFPNVYTLFTSMFVHSDFLHILGNMFVFFFMGV
                     AFEQRVGPKKFLLIYLVTGVCGALTHSLLNIGSTIPLIGASGAIFGILGAFAYAYPRD
                     EIVMPVPIGIMFIMRIKVIYATIIFAVFETVIVMFSVQDSTAHFAHFGGLASGVVLAA
                     ILLRNRAEKQTQTKGVSPVDYMQIKKDDTINFSHLTKLVKTSEEKKILQRIEKEDVLQ
                     VRDMWLEHFLEKITCPVCNKPLNHLGRKIWCEQNHFQTDY"
     gene            complement(4020..4871)
                     /locus_tag="VN377_00510"
     CDS             complement(4020..4871)
                     /locus_tag="VN377_00510"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HWR26897.1"
                     /translation="MGCFFVIINGFALLIKNLFQPTFEDASNPVNILYIFAIIIVMTV
                     IILIIARYWKKQVIQAIILFAIGYTAFFIFVPLFGFLPEMLSIAVSLIIAVALVVALY
                     KYPEWYVIDLCGVIVGAGAIAIFGISLSIFLVIILLVVLAVYDAISVYQTKHMIDLAD
                     TVMDLKLPVLLVVPKIRRYSLINETKSLKEKLKEHEERDAFFMGLGDIVMPGILVVAA
                     YQNIPNGLPVALSVLIGTLIGFLVLMTFVIKGKPQAGLPLLCSGAILGYFISSYVFFG
                     AFPGFGF"
     gene            5139..6515
                     /locus_tag="VN377_00515"
     CDS             5139..6515
                     /locus_tag="VN377_00515"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR26898.1"
                     /translation="MKERGRKKNILRNISTLVIVCALIGCSFSALTITTIGTAKQNST
                     LKTTSSKSSGSQISSSVNFNLLDENFTDGNMPPEGDSGNWELQQTNPDETWYIDSTVP
                     YTRPFCGTIHRDGSQTLQDEWLITPSLNFIGDAHNYTSIALSFHWYTCYYTTVYKRYV
                     ELNISISTDGGANWTNIWSFDDMNVGIPPNPFTDWKWYESNYLDKKPIDLTDFIGEND
                     VRIAFQYYSDTNASADQQEFSIDDINVIATGPGGIFSCNAGGPYNWWWPMQYEYFPNG
                     VRFHGNATNGTIFTQFLWDFGDGSTNVTSYNQNPTHFYSDIGIFNVTLTAKDNTFTPP
                     VINVSRTTLTLFLLKPPAVNITAPRISIGIKAVINNVGEYNATYVKWMINISWGLLQL
                     RVKPVANGTLENIEAGGSATIQSKPYFFGFGLIHIIITAYPENQPSLIKHLYGFKIGP
                     LVFVAQNM"
BASE COUNT         1962 a         1484 c         1378 g         1729 t
ORIGIN      
        1 cattaaaagg gtttatgtca aaagagattg ttaatttaga taagctgtat aaggaatttc
       61 tagatttatt tctagatgta tcaagagaga tagaacggtt aaaaatttta ataaatgata
      121 ctacgaaagc gagagttaaa catagagctt aggaaataaa ttaagaaaaa gattacattc
      181 accaatcgtt actttagtat cggcaaccaa tactgcacca tcaatgcatc tggcttcaag
      241 aatatagatc acgtcattct tgaatttcaa gaaattatat attctttccc atttagatta
      301 gctataccgg aatagccgtg gtttaggtaa taaattaacc ttgtcataat gagcattcga
      361 agtccatcaa taaaaatcgg tctttttgaa aggaattgta aggttataaa taaccaaaag
      421 tgttaaaaca gtccactgaa tattcgggtt acacctaggc catataacaa cgtccacggc
      481 catataacac gtttaccatc aacaggagaa tgggcgggta acaaagtggc tatgtggcgg
      541 actgcagatc cgcctacgag ggttcaaatc cctccccgcc ctctaacaaa aaaatagtct
      601 gggagaaatt tgtatgattc gaaaaaaaac ctttgtggat atggtaattt tttattgtgt
      661 cttctcaggt cttgtattac cagtaatacc tgaaccggtg aagtcagcat ctggagagac
      721 gctcctgatc aatgaagtaa tgttccatcc gatcgaagat gaaaatacga acgaatggat
      781 cgaactgtac aatccaacca cagaagctct tgatgtcgaa ggatggatga ttgctgatga
      841 aaaagaaaca gatatcattt tagctgatac tgtgaatggt gatgggtcca ccagcattcc
      901 tccaggatgt tatgcgatac tcaccgataa agagacaacc atctatgaga cctatatcat
      961 ccttgataat gcagtgagat tatctgtcga tgatagcacc ctgtgtgggt acgggttgaa
     1021 caacaaacaa gaaaaaatca tcctctctga ctcggatggc gatctcgtcg atgcgatcga
     1081 atggggtgag gactacgatg atatccctgg tagtccgttt gtttttgttt cagaaggaaa
     1141 cacactggct cgttaccaag gaaccgatac ggatgacacc tctgttgatt tctttgaatg
     1201 cagttctcct tcaccaggga gtgaaaatat ccaaacgatg cgagaggaac aaccagaggt
     1261 aactgcatcc gaagaggaag aatctgaaga aaccacagga gacgttcctc ctcagattct
     1321 gattaccgaa ctgtattacg atgctcatcc aactatcaat gctgaataca tcagactttt
     1381 caatcccatg aacaagtcga tagatgtctc cggttggtac ctcaccgata aaccatggaa
     1441 agagcctgat gatcaatcaa aaatactctt cccagaacat acgttcatgc tgccgcagac
     1501 gtcatggttc atttcaaaaa atgcttctgc ttttttctgg gaaactgcca tgcttcctga
     1561 tttcgaatac ggggtggact cccaactggc tgtccctcag cttctcacct ttagaaccgt
     1621 caccttcagt aacaccggcg gccttgtcgg gttatacact gcagcacaaa gccttgtcga
     1681 tctcattatc tacggagaaa ccgatgagta cgtctcagga tgggaaggac cttcgattcc
     1741 cccctcagga caaggagtca tcctgaagcg gaacagcatc aatggaacag tggtcgatac
     1801 ggattctgca tcggattgga tgcaccatcg aatctaccag atcggacaat cggatttacc
     1861 ccttcaaacc atagcatcaa ccggagagat caccaccttc gtctcaccgg ataacagtta
     1921 cgataccata acacgtgaac tccgcgatgc ccagtattca atcgatatga acatgtatga
     1981 attcaccaac ccgttcctct atcaagaact cctcgacgcg ctcaaacgga atgtcacggt
     2041 cagaatcttc atggaaggct cccccatcgg aggcattgac gaccgggagg gattcatcct
     2101 tggcgacctc gctgcccatg gaggcctgat tcgtttcatt gtcagcgatg acgagaacca
     2161 tgtgaacgcc cgttatcaat tcgaccatgc gaaatatctt ctcatcgata atgaaaccgt
     2221 gatcgttgaa agctgcaact gggcgaaaac cggtgtccca aaaaatccga cctatgggaa
     2281 cagagaatgg gggatcgtcg ttcgaaacaa agaagtggcg actgggtttt ggcaggtgtt
     2341 tcaggatgat tgggacccgc tgcatacaga tagctgtccc atcgaagcaa tgaacttgac
     2401 tttttaccct gattttacgc tggactatga ggttccgacc ggctcgtatc ttccacagtt
     2461 cgatgcaaag accatcactg gaccttgcac aatcacaccg gtgttttctc cagataccag
     2521 tgagcaggtt ctccttgatg cgatagatgc tgcgacatcc accatctatg tccaacaact
     2581 gtacatctac agagactggg gtgagacgac aagcccgctt gtcgaacatc ttgtgaacaa
     2641 atcattagaa ggcgtcgacg tccaggtaat cctcgattat cagacagatt acgaagagac
     2701 aattacgaag cttgatgaaa ccaaggagtt tttggaaacc tacggggtca gagtgaaatt
     2761 catctcaagc gaatggtcac ctttctccac catccacaac aaaggcatga tcatcgataa
     2821 caccactgtc cttatctcaa gtatcaactg gaacgaacaa tcagtcagaa agaaccgtga
     2881 ggcaggactc cttgtcaaca gcgaagaagt tgcaacgtat tatgcgaccg tgttcctctc
     2941 cgattggaac cttgaagcct ccaaaacaga cacatcattt ttctcgtggg cagactataa
     3001 atacctcgtg cttattgccg tagtggtttg catcactctt gtgttcatcg cacgagattg
     3061 gagaaaacga aaatggacat agcatttctt caaccctcct ggatttcgct tattgctatt
     3121 ggcatcatgg ttgggacact cgtcattgct tttctcaaaa agattaccct cacctactcg
     3181 attattatcg cttgtttctt tgttttcctg atcagcttgt tctttcgaac ggaaatcatc
     3241 aacgatctag ggtttcgacc gagctatctt tctttagagg cgttcccgaa cgtgtacacc
     3301 ttgtttacct ccatgttcgt ccatagcgac tttttacata tccttgggaa catgttcgtg
     3361 ttctttttca tgggtgtcgc ctttgaacaa cgggtaggac caaagaaatt ccttcttatc
     3421 tatctggtca ctggcgtctg tggcgcgttg acacattccc tgttgaacat cggttcgacg
     3481 attccgctta ttggtgcctc gggagcgatt tttggtatcc ttggagcgtt tgcctacgcg
     3541 tacccccggg atgagatcgt catgccggtc cccatcggca tcatgtttat catgcgtatc
     3601 aaagtcatct acgctactat catttttgct gtctttgaga ccgttatcgt gatgttctct
     3661 gtgcaggaca gcaccgctca tttcgcgcac tttggaggac tggctagcgg ggttgtcctt
     3721 gctgcgatcc tgctgaggaa cagagcggag aaacagacgc agacaaaagg cgtcagtccg
     3781 gtggattaca tgcaaatcaa aaaagatgac accattaatt tttcacatct gacgaaacta
     3841 gtgaaaacct ctgaggaaaa aaaaatactg caacgcatcg aaaaggaaga cgtcttgcaa
     3901 gtgcgagaca tgtggcttga gcatttcctt gagaagatca cctgccctgt gtgtaacaaa
     3961 ccgttaaacc atctaggcag gaagatttgg tgtgagcaaa atcatttcca gaccgattat
     4021 taaaaaccaa aacctggaaa ggctccaaaa aagacgtaac tggagatgaa gtatccaagg
     4081 atcgccccgc tgcagagcaa tggcagccct gcctgaggtt ttccttttat cacaaatgtc
     4141 atgagcacca ggaaaccaat caatgttcca atgagtactg agagggcgac tggcaatcca
     4201 ttcggtatgt tctgatatgc ggcaaccacc aggattcctg gcatgacgat gtctccgagc
     4261 cccatgaaga aggcgtcccg ctcctcatgt tctttgagtt tttcctttaa gctctttgtc
     4321 tcattgatta acgaatagcg tcgtattttc gggaccacca gtaacacagg taatttgagg
     4381 tccatcaccg tatcagcaag atcgatcatg tgttttgtct gatagaccga tatcgcatcg
     4441 tacaccgcca agacgacaag caggatgatg acaagaaaga tgctgaggga aataccaaag
     4501 atagcgatag cgcctgctcc gacgatcacc ccgcacaggt cgataacgta ccattcagga
     4561 tatttgtaga gtgcgacaac aagagcaacc gcgataataa gagaaacagc aatggaaagc
     4621 atctcaggta agaacccgaa caggggaaca aaaataaaaa aagcggtgta cccgatcgca
     4681 aataaaatga tggcctggat cacctgtttc ttccaatatc gtgcgatgat cagaatgatg
     4741 accgtcatga cgatgatgat cgcaaagata tagaggatat tgaccgggtt gcttgcatcc
     4801 tcaaacgttg gctggaagag gttttttatc aatagcgcga acccattgat gatgacaaag
     4861 aaacatccca tgatgaaaac cggcaagatc atttttttat cagtttcttt ctcgggagtc
     4921 attcgtaccc aagaatagtc tgttcctttt tatatggttt gtttttaaaa aaaaatgaat
     4981 agttttttgc atcttcgctg caagttatct tctcgattgt gaacgttctc taagtaaatt
     5041 attgtaacaa aagttacaat taaattccta taaaatgatt tatatacaga caaaatatat
     5101 tgcatccaca ggaattatca aagggaggag tacccattat gaaagaaaga ggaaggaaaa
     5161 aaaatatttt aagaaatata agtacgctag tgatcgtctg tgctctcatc ggttgtagtt
     5221 tctctgcatt aaccattacg acaatcggta cggcaaaaca gaacagtacc cttaagacaa
     5281 cctcatctaa atccagtggt tctcaaatat cctcatcagt caatttcaac ctcttggatg
     5341 aaaattttac tgatggaaac atgcctccgg aaggcgattc gggaaattgg gaactacaac
     5401 aaaccaaccc tgacgaaacc tggtatatcg actctacggt cccctacacc agaccattct
     5461 gcggcactat ccatcgtgac ggaagccaaa ctctccaaga cgaatggctc atcaccccaa
     5521 gcctcaattt cattggtgat gcccataatt acacaagtat tgcactaagt ttccattggt
     5581 acacctgtta ttatactacg gtatacaagc gctatgtcga actcaatatc agtatctcaa
     5641 cggatggagg agcgaactgg acgaacatct ggagttttga tgacatgaac gtcggaatac
     5701 ctccaaatcc ttttacagac tggaaatggt atgagtcaaa ttatcttgat aaaaaaccca
     5761 ttgacttaac tgatttcata ggggaaaacg atgtacggat tgcattccaa tattatagtg
     5821 acactaacgc atcagcagat caacaggagt ttagtatcga cgatatcaac gtgattgcta
     5881 ctggcccagg aggtatcttc tcatgcaatg ctggcggccc ttacaattgg tggtggccta
     5941 tgcaatatga atattttcca aatggcgtcc gattccatgg gaatgctacc aacggaacaa
     6001 ttttcacaca atttctttgg gatttcggcg acggcagtac aaacgtcact tcgtataacc
     6061 aaaatccgac ccatttttat tctgatattg ggatcttcaa cgtgaccttg acagcaaaag
     6121 ataatacgtt tacaccgcct gttattaatg tctcccgaac aactctaaca ctattcttac
     6181 tcaaaccacc agcagtaaat atcaccgcac cacggatttc aattgggata aaagcagtca
     6241 tcaataatgt cggagaatac aatgctacct atgtgaaatg gatgataaat atctcctggg
     6301 gcctattaca actacgcgtg aagcctgttg caaatgggac tctagagaac atagaggcag
     6361 gtggttcagc gaccattcaa agtaaacctt acttcttcgg atttggcctt atccatataa
     6421 taattacagc atatccagaa aaccagccga gtctcatcaa gcatctctat ggatttaaga
     6481 tcgggccctt ggtctttgtt gcacaaaata tgtaaatcca atatatacct aggagtacat
     6541 ggtaccccta tcc
//
LOCUS       DATMXJ010000014         9425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_52451, whole genome shotgun sequence.
ACCESSION   DATMXJ010000014 DATMXJ010000000
VERSION     DATMXJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52451"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="VN377_00520"
     CDS             complement(<1..1531)
                     /locus_tag="VN377_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR26899.1"
                     /translation="MLFSVVPSSILGQQNADENQTQYQKTFTLTFSKNDFSFNTQNGY
                     DTVDLSDGGVTTEIGKPLLPVKNIKIALPADMKITNVRINELKQQTVAGTFIMYPAQP
                     SLQSNQTENEVQFVQPDTKTYQSNQIYPAQYIQASGQNDLAGQSIAEVTIYPLHYIPM
                     QKKLTAISSITFTIEGTNGYLCGDYLSEHISESSRDLYQHMIQNMVINPEDVVLRTSQ
                     SPQSSSVDPGNYDYVIITQDSWVSAFQPLAEWKTKKGIPANIVTTTWIYNSGGYSGSN
                     VDKIRAFVLDVYTNWGTIYVLLGGDVDVVPCHYRTFSQVDPDPVPNDAYYADFDSDWV
                     CEVNVGRASVTGTGSGTGKIGNFINKILTYEKNPSLTDFAKKAGLYGFDLDGSTPAEQ
                     CKIYIDNTFIPSSWTMTNVYDSQSGNHKTNVIASLNAGQNLMNHADHSGSDFMGTGYV
                     NHDIGLWNSDMDELTNGNKQGVFYSMGCDPAAYDTSNCIAEHFVQNSNGGGVAFIGNS
                     RY"
     gene            2161..2646
                     /locus_tag="VN377_00525"
     CDS             2161..2646
                     /locus_tag="VN377_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26900.1"
                     /translation="MKYTLRKRYFLENEEAVSEEFTVLPALSVVMIGFALFVVLLAQT
                     YLAYTDRIDRLQTYQTADVLLQKLTNPDCFFIRESGLVDVHLLQNDTSLLKNFFERYT
                     KAGLSFLLQLHWNDQLWEYPNTLELNPLIRTAVSKEIGVYLNEAQTIPGTLTIVIWKG
                     S"
     gene            2646..3977
                     /locus_tag="VN377_00530"
     CDS             2646..3977
                     /locus_tag="VN377_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26901.1"
                     /translation="MKQIRFLDDVFGISMMYDAVFFIIMVSLTGVILLPVLRTDIALE
                     SSVDKHREEIVDETLHTFLVTRADSFEYRFGGNLIDEIAGHIGIDNTSNGLYGSVTHW
                     LLAHEQRHKTYATLLAEYLGCQFRLPFSFIGTHQINLFVGDFERQLSNETDRFFSSLL
                     GEKYRYNLSAWWHPVRGVPFGGEFSIGEHPPAKDCYVAQSFFMMPYTPVFSVGNHTII
                     FTKHWLKHQLFDHDVGLGRSSIPAIANITIVLENYTNKQPPYDIRENAAKATKENLSD
                     LIYSFLIDGITNESNVTVFPGIVHTTLLYGFEKLKNMTGQFLDKALNESFGASIRSID
                     RLFSGLNSSAMNPLSQAILMQLNATLGDMLNGSFDSLNDAFDACESAIKERIILLIQG
                     YIDSLLEAFVSTLFDVIDSIMDFSEMLIDLLFDCISLNKAEVMLTIWVVRE"
     gene            3959..6736
                     /locus_tag="VN377_00535"
     CDS             3959..6736
                     /locus_tag="VN377_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26902.1"
                     /translation="MGGERIIRRKFFSEQHARIPFSIIGVFLLLGSSVTAVYISRLEL
                     EKSKEIARSLDVNEIENLLYYFEADLTNALNLAGMKALKEVGKHPVIISSLGTGQEIN
                     LYRVKAIIKEELNVYLTGHYLYNMFCDGHYAINVMLQNETTILSIENISLEPCSMQLE
                     RFSIPSIGPSGTMNYSTYLIASLPLQIEIRQLKQYDWDLLTTRTVRISSILTSRYPLL
                     ECLMDEYNRTINGTFSSLWTYTTMLSNLYSLLRGFKHFRCGRPLNVMDNRHLSVILNS
                     GLLLEQGLVFGSADPLGFVDLAREIKQTLKQTPSEALTTFNDEMEGEGYSVEAENLSK
                     GSANAEAGSPINESIDISASFNLSEIAERILYTITSAIFHFENSEGEFYDENILFDGD
                     VESRINEVIQQQANQSFYLTRITKHLSVNATTLQFLESLISEMYRDTMSTQVFDRSVI
                     VELWGDPGEGWIDGGYSPWNATSILPVSEQEIKPQKGLVTPGCAVYEEHYNVSYERNH
                     TWWRLEEHSVNGTTMQITIWNNKTDLLIETVVLRSLLQHWASYQATQDDIVDILYLNE
                     ILNDPNLEDTVLRYLATYNDSGMEKQLLITTRNNTGTIGLDAVVHGMYAEWVLDEVWS
                     ALEEIFESVQHITLNPDINAERYPNPVQLVESAKNDLLIKYTQNTSNYLDFSSYHPST
                     QFLSVGKKAVYYSREWYVRTVKNTTDSVFTQISQQLTHAIEAAIPSSAGFTTKNITET
                     IEDASDALRNQFTIPFGYDMNLSRYDRLGVLLWNETIRLAVDQYPNYLNPFERTSWNS
                     EELWTLKIRNRCVFGPTGLPLLPPSPVTAWVLTMNLWVIDVQGEYPQFKIIDTSDETI
                     FNPLLGHEPQSYVRELKVISTGNITLGRNTRLSFGFTTVAFGLVPPWGMMLGDIQENW
                     FDEHTSGFNEE"
     gene            6742..7371
                     /locus_tag="VN377_00540"
     CDS             6742..7371
                     /locus_tag="VN377_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcinarray family MAST domain-containing
                     protein"
                     /protein_id="HWR26903.1"
                     /translation="MIRNLGTIFFIGLLILLSTAPNHSAGECTYGSVHAWFRTSDGEW
                     ENATAHPMLSRGETFEIKIMVQVKTDLRVVYLTLHEFGTPVYEVITGPSIMEQLLEHW
                     GPTRPDQSWIHVWKMRVRANTSWVNGYSPLEIYVQFTKNDADDSSVTFDVLNAFIVDE
                     LWEHYSEETSTENISSQRKDVPRLSDASIIGILIIFFLAGIYLRTRQLR"
     gene            complement(7389..7832)
                     /locus_tag="VN377_00545"
     CDS             complement(7389..7832)
                     /locus_tag="VN377_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR26904.1"
                     /translation="MKIRCAQKRDAEAITAQNIALARESENIILNHETTLAGVNALLS
                     DKKKGLYLVAEENKTIVGQLMITVEWSDWRNKPIWWVQSVYVQKTWRKQGVFTHLLNE
                     AKDMARQQGVAFLRLYAHQNNKPALAVYEQIGWRQDPYTVYHLHL"
     gene            complement(7881..9239)
                     /locus_tag="VN377_00550"
     CDS             complement(7881..9239)
                     /locus_tag="VN377_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR26905.1"
                     /translation="MFYGLAPPLGLLYAATMLQNEGDSVTILDFSAEPFDEQRLITTL
                     HQSDVVGMSVLSPSLNEVKTLIQLITQHKPDIPIIIGGPHCTLRPEKALDETQATVCV
                     QGDSEMILTDLKKALNKEKDFSEIPGIYYRSSHDIRQGTAPQMIQDLDSIPFPARHLV
                     KHYTYGREYNPHLKAGEFTSIITSRGCPYTCRFCSRGSISMQRYRTRSTENIISELKE
                     IQQQGYRHVVFSDDCFPANKKQASALFDAVIDEDLSLKFSVTATRVDLADKQLYKKMR
                     LAGVTHIQFGLESGNQDVLDFYHKNTTVETIRNAVQLSHDTGFFTIGSFIFGAPFETN
                     EHFNNTLRFAKSLPLDSVSFLPLRYMVGSELWDQAVSDGKINANEYLVSADKNRGLGN
                     YTKEDLLAYCMRAQRSFYGRPSFFINLLKKSLRNDDLSYVQSYLAILSSSLKGIFQKK
                     LS"
BASE COUNT         2600 a         2026 c         2079 g         2720 t
ORIGIN      
        1 cgtatcgcga gttcccgata aaggcaactc caccaccgtt gctgttttgg acgaaatgtt
       61 ccgcgataca attcgatgtg tcatacgcgg cagggtcaca ccccatggaa tagaagaccc
      121 cttgtttatt cccattcgtc aactcatcca tatctgaatt ccaaagaccg atgtcatgat
      181 tcacataccc ggttcccatg aaatctgagc cagagtgatc agcatgattc ataagattct
      241 gccctgcgtt gagagaagcg atcacatttg ttttatggtt tccgctttgg ctgtcgtaca
      301 cgttcgtcat cgtccaactt gaggggatga acgtgttatc gatatagatc ttgcattgtt
      361 ccgcaggagt gctgccatca aggtcaaaac catagagtcc ggctttcttt gcaaagtcag
      421 tcaacgacgg gtttttttca taggtgagta ttttattgat aaaattacct atcttaccgg
      481 tcccactccc tgtgcccgtc actgatgcgc gaccaacatt gacttcacag acccaatcag
      541 aatcaaaatc agcgtagtac gcatcgtttg ggactggatc tgggtcaacc tgggaaaagg
      601 ttctataatg acaggggacg acatcgacat ctcctccgag aaggacgtag atggtacccc
      661 aattcgtgta gacgtccagg acgaacgccc ggattttatc gacattggac ccgctgtatc
      721 caccactgtt gtagatccat gttgtggtga cgatatttgc cgggatgcct ttttttgttt
      781 tccattctgc gagcggttgg aatgcgctca cccagctgtc ctgggtgatg atgacatagt
      841 catagttacc tgggtcaaca ctagaggatt gcggactctg agatgttcgc agtacgacat
      901 cctctgggtt tatcaccata ttttggatca tgtgttggta caagtctcta ctactctccg
      961 atatatgttc tgagagataa tcaccacaga gatacccgtt cgttccttcg atggtgaagg
     1021 ttatcgagga gatcgccgtg agtttctttt gcatagggat atagtgaagc gggtagatgg
     1081 ttacctcagc aatgctttga ccagcaagat cattttggcc gctcgcttgg atatactgtg
     1141 cggggtagat ttgatttgat tggtaggttt tggtatctgg ctggacaaat tgcacctcat
     1201 tttcggtttg gttactttgc agtgatggtt gcgcaggata catgataaat gttccagcta
     1261 cggtttgttg tttgagttcg ttgatccgaa cattggtaat tttcatgtcg gctgggaggg
     1321 cgatttttat gttttttaca ggcagcaaag gttttcctat ttcagtagtt actcctccgt
     1381 cagataaatc aacagtatca tatccatttt gagtgttgaa agaaaaatca ttttttgaaa
     1441 acgtaagcgt aaatgtcttt tgatattgcg tttgattctc atctgcattt tgttgtccca
     1501 gtatagacga tggtacaaca gaaaataaca tactacttat tatcaaagat aatacgatag
     1561 tctttctgaa ttttttgtat ggaattttat atgcccccta attatgttat tgtagggctt
     1621 ggagatattt aaacttaacg aatgttaaga acaaaaatat tttttttagt attgaacccc
     1681 taagcgctat ggtaaaaaca aaatcgaaaa acacccagag gagagcccaa cgaggtacca
     1741 aataccacgc ggctccatta aagacagcca ggacagcacc aaataccgcc aaaccgcctc
     1801 taggcatgtt attctttgtc attcataata tataaaccta tcctgacaaa acgacaattg
     1861 agataaaaac caaggagata atcaataaca tcgacaaagg ggaagaaaat ttttaaccca
     1921 tcgaaggttt tagaacacta cctctatcaa aagaaaatcg gtgaaacatg atccgatttg
     1981 acgacataga ggcacaggaa acctccggtg ccctaccgga ggtttctcat ttagaatttc
     2041 ccaatcgtga ctctacaaca attcgtcttc gtatgccatc ataaaaaaaa cataattttt
     2101 atatatgata cattctattt aaaattggat attcaacaaa aaagtctggg ggaattagaa
     2161 atgaaatata cattaagaaa gcgttatttt ttggaaaatg aggaagctgt ctcagaggaa
     2221 ttcacggtcc ttcccgcact atcggtagtg atgataggat tcgcactctt cgtcgtcctc
     2281 ttagctcaaa cctaccttgc atacacagac cgtatcgatc gtctgcaaac atatcaaacc
     2341 gcggatgttc tcttacaaaa actgacgaac cctgattgtt ttttcatcag ggaatctggt
     2401 cttgtcgatg tacatctact gcaaaatgat accagtttat taaaaaattt ctttgagcgg
     2461 tatacgaagg cagggctctc ttttctttta caacttcact ggaacgatca actctgggaa
     2521 tatccaaata ctctagaatt aaatcctctc attcgtaccg cggtgtcaaa agagataggc
     2581 gtttatctga acgaagcaca aaccatccct gggacattga ccattgtgat ttggaaggga
     2641 tcttgatgaa acagatacgt ttccttgatg atgtgtttgg tatttcaatg atgtacgacg
     2701 cggtattttt tatcatcatg gtgtctctta caggagtgat tcttcttccg gtgttacgaa
     2761 cagatatcgc attggaaagc tcagtggaca aacaccgtga ggaaatagtc gatgaaacct
     2821 tgcatacctt tctggtgaca cgcgcggatt ctttcgagta tcggtttggt gggaatctca
     2881 tcgatgaaat cgctgggcat atcgggatag ataacacttc gaatggtctg tatgggagtg
     2941 ttacccattg gttgcttgcg catgagcaac gccataagac gtatgcgacg ctacttgctg
     3001 aataccttgg atgtcaattc agattgcctt tctctttcat tgggactcat cagataaact
     3061 tattcgttgg tgattttgag cgacagttga gcaatgaaac cgaccgattt ttctcctctc
     3121 tgcttggtga aaaatatcgg tataatcttt ctgcctggtg gcacccggtc agaggagtgc
     3181 cctttggggg tgagtttagc attggtgaac atccaccagc aaaggattgt tatgttgcgc
     3241 aaagcttttt catgatgccg tatacgccgg tattctctgt cggtaaccat acgataatct
     3301 tcacgaaaca ttggttgaaa catcaactct tcgaccatga tgttggactg ggcaggtcat
     3361 cgatcccagc gattgcgaac atcacgattg ttcttgagaa ttataccaac aaacagccgc
     3421 cgtacgacat acgggagaat gcggccaaag cgacaaagga gaatctcagt gacttgatct
     3481 atagttttct catcgatgga atcaccaatg agtcaaacgt gaccgtgttc cccgggattg
     3541 tccataccac tctgctctat ggttttgaga aattaaaaaa catgactggt cagttccttg
     3601 ataaagcgtt gaacgaatca tttggggcct cgattcgatc aatcgatcgc ctctttagcg
     3661 gtctgaattc aagtgcgatg aatccgctat cacaagcgat tctcatgcaa ctgaatgcaa
     3721 cccttggtga tatgttgaat ggatcgtttg attcgttgaa tgatgcgttt gacgcctgtg
     3781 aatcagcaat caaagagcga attattctcc tcatccaagg atatatcgat tccttacttg
     3841 aggcgtttgt cagtactctt tttgatgtca tcgattcgat catggatttt tcagaaatgc
     3901 tcatcgattt gttgttcgac tgtatctctc tgaataaagc tgaagtgatg ttgaccattt
     3961 gggtggtgag agaataatcc gacgaaaatt tttttctgag cagcatgccc gtattccttt
     4021 ctccatcatt ggagtcttcc tccttcttgg aagcagtgtc actgcggtgt acatctcgcg
     4081 attggagctg gagaagtcca aagagatagc ccgttccctt gatgtaaatg aaattgaaaa
     4141 tctactctat tatttcgagg ctgatctgac aaatgcattg aaccttgcag ggatgaaagc
     4201 gttaaaggaa gtcgggaagc acccggtcat catctcttcg ctaggaaccg gccaagaaat
     4261 aaatctgtat cgagtgaaag ccatcatcaa agaggaactg aatgtgtacc tcacgggtca
     4321 ttacctctac aatatgttct gtgatggtca ttatgctatc aatgtcatgc ttcagaacga
     4381 aaccacgatt ctctcgatag aaaacatcag ccttgaacca tgttcgatgc agctggaacg
     4441 attttcgatt ccatcgatcg gaccgtctgg aaccatgaat tattccacgt acttgatcgc
     4501 ctcactccct ttgcaaatcg agatacggca attaaagcag tacgattggg atcttctcac
     4561 aactcgaacc gttcgtatct cttcgattct tacctcacga tatcctcttc ttgaatgtct
     4621 gatggacgaa tacaatcgga caattaacgg cacgttttca tcattatgga catacaccac
     4681 gatgttatca aacctgtata gcttactcag aggattcaaa cattttcgtt gtggacgacc
     4741 gttgaatgtg atggacaacc gtcatctttc agtgatcctc aacagtggat tgctactgga
     4801 acaagggttg gtgtttggca gtgctgaccc cctcggattt gtcgaccttg cacgggagat
     4861 aaaacagacc ctaaagcaga ccccttcaga agctctcacg acctttaatg atgagatgga
     4921 aggcgaagga tactctgttg aggctgaaaa cctttcgaag ggtagtgcga atgctgaagc
     4981 aggatctcca atcaacgaat ccattgacat ttctgcttcc tttaatctct ctgaaatcgc
     5041 agaacgaatc ctatatacta tcactagtgc aatcttccat tttgagaaca gtgaaggaga
     5101 attctacgat gaaaacatcc ttttcgacgg tgacgtcgaa tctaggataa atgaagtgat
     5161 acaacagcaa gcgaatcaat cattttatct cacaaggatt acgaagcatc tcagcgtaaa
     5221 tgcgacgact cttcagttcc tggaatctct catctccgag atgtaccgtg atacgatgtc
     5281 gacacaggtc ttcgatcgtt ctgttattgt ggaactgtgg ggagatcctg gggaaggctg
     5341 gattgatggc ggatattcac cgtggaatgc aacctccatt cttccagtct cggaacaaga
     5401 gataaaacca caaaaagggc tggtgactcc agggtgcgcc gtatatgaag aacattataa
     5461 tgtctcctat gaacgaaatc atacctggtg gcgacttgag gaacactctg tcaacgggac
     5521 gacgatgcag ataaccattt ggaataataa aactgatttg cttatcgaaa ccgtggttct
     5581 tcgaagtcta cttcagcact gggcgtcgta ccaagcgact caagatgata tcgttgatat
     5641 cctctatctc aacgagatac tcaatgaccc gaatctcgaa gataccgtac ttaggtatct
     5701 cgcaacgtat aatgattcag gaatggaaaa acaactgttg attacaacac ggaacaatac
     5761 aggaaccatt ggtctcgatg cggtagtaca tggaatgtat gcagaatggg tcttagacga
     5821 ggtttggagc gcccttgaag agatatttga atcggtacag cacatcaccc ttaatcccga
     5881 tattaatgca gaacgttacc caaaccctgt ccagcttgtt gagagcgcaa aaaatgatct
     5941 tctcataaaa tatactcaaa acacttcaaa ttatcttgat ttctcttctt atcatcctag
     6001 tacgcagttt ctcagcgtcg gaaaaaaagc ggtgtattac tcacgcgaat ggtacgttag
     6061 gaccgtgaag aacaccaccg actctgtttt tacgcagatc tctcaacaat taactcatgc
     6121 gatagaagca gcaataccat cctcagctgg ttttacaaca aagaatatca cggaaacaat
     6181 agaggatgca tcagatgcgc ttcgaaatca attcacgatc ccttttgggt atgatatgaa
     6241 tctctcacga tatgatcgcc taggagtgtt gttgtggaat gagacgatcc gtcttgcggt
     6301 ggatcaatac ccgaattatc tcaatccttt tgaaagaaca tcatggaaca gcgaagaact
     6361 ctggactctg aagattcgta atcgttgcgt atttggaccc accggtcttc ccctactacc
     6421 tccctcaccg gtcacagcct gggtgttgac catgaatctc tgggtcattg atgtgcaagg
     6481 tgaatatccg caattcaaaa tcattgatac cagtgatgag acgatcttta atcctttatt
     6541 aggacatgag cctcaatcat atgtacggga attaaaagtc atctccacag ggaacatcac
     6601 acttgggcga aacacccgtc tctcctttgg ttttacaacg gttgcgtttg ggcttgtccc
     6661 cccgtggggg atgatgctag gggatattca ggaaaactgg tttgatgagc atacctctgg
     6721 atttaatgag gaatgatccg tatgatcaga aaccttggga cgatcttttt catcggtctt
     6781 ctcattcttc tgtctacagc cccgaatcac agtgcaggtg aatgcacgta cggatcggtt
     6841 catgcgtggt tccggacgtc tgatggagaa tgggagaatg cgaccgctca tccaatgctc
     6901 agccgtgggg aaaccttcga gataaaaatt atggtgcaag taaaaaccga tctgcgagtc
     6961 gtctacctta cactccatga atttggtaca cctgtgtacg aggtgatcac cggaccatcg
     7021 atcatggagc aactgctcga acactgggga ccaactcgac ctgatcaatc atggattcat
     7081 gtctggaaga tgcgagtgag agcgaacaca tcatgggtaa atggatacag ccccttggag
     7141 atatacgtac aattcacgaa aaacgatgct gacgattcat cagtcacctt tgatgttctg
     7201 aatgctttta tcgtcgatga actctgggaa cattattcgg aggaaaccag taccgaaaat
     7261 atttcatcgc agagaaaaga cgtacccaga ctttccgatg catccatcat agggatactc
     7321 atcatctttt ttctcgcagg tatctatctt cgcacccgcc agctaagata acaagagttt
     7381 tttaatcctt acaagtggag atgatacacc gtgtaggggt cttgtcgcca tcctatttgt
     7441 tcatacaccg caagagcagg cttgttgttc tgatgagcat agagccgtag aaatgcaact
     7501 ccttgttgac gtgccatgtc tttcgcttcg ttcaacaagt gagtgaacac cccttgcttc
     7561 ctccatgttt tctggacata gacactttga acccaccaga ttggtttgtt cctccagtcg
     7621 ctccattcca ccgtgatcat aagctgtcca acaatcgtct tgttttcctc tgcgacaaga
     7681 tacaatcctt tctttttatc ggacaacaag gcgttcacac ccgcaagagt cgtctcatga
     7741 ttaagaatga tattttcgga ttcccttgca agtgctatgt tctgtgcagt gattgcctca
     7801 gcatctcttt tttgcgcaca tcgtatcttc atatgaagaa ataagaacga aaagttctct
     7861 tttaatattt cgaaaaaaca tcaagataat tttttttgga aaattccttt caaagacgaa
     7921 gaaaggatgg caagatagga ttgtacatag ctaagatcat cattacggag ggattttttc
     7981 agaagattga tgaaaaatga tggccgccca taaaaagaac gctgtgccct catgcaatat
     8041 gctaaaagat cttcctttgt gtagtttcct aaaccccgat ttttatcggc agatacaaga
     8101 tattcatttg cattgatctt cccgtctgag accgcttgat cccatagctc agagccgacc
     8161 atataacgca acggtagaaa tgagactgaa tcaagaggca gtgattttgc aaaacgcagt
     8221 gtgttgttga aatgttcgtt cgtctcaaaa ggtgcgccga aaataaatga gccaatggta
     8281 aaaaacccgg tatcgtggct taactggacc gcatttcgta tggtttcgac agtagtgttt
     8341 ttgtgataga aatcaagaac gtcctgatta ccagattcca aaccaaactg gatatgagtg
     8401 acacccgcta atctcatttt tttatacagt tgcttgtctg caaggtcgac tcgtgttgcg
     8461 gtcactgaaa atttcaaaga aagatcctca tcgatgactg catcgaataa agcagatgct
     8521 tgtttcttgt ttgcagggaa acaatcatct gagaaaacaa cgtgtcggta gccttgctgt
     8581 tggatttctt taagttcgga gatgatattt tctgttgaac gagttcggta tcgttgcatg
     8641 ctaatggagc ctcgggagca gaaacgacag gtatacgggc atcctctgct tgtgataatc
     8701 gaggtgaact cccctgcttt cagatgaggg ttgtattccc ggccatacgt gtagtgtttc
     8761 acgagatggc gggcagggaa agggatgctg tcgagatcct ggatcatttg cggggcggtt
     8821 ccttgtctga tgtcatggct tgatcggtag taaatccctg ggatttctga gaaatctttt
     8881 tctttgttga gtgctttttt caggtctgtc agaatcatct cactatcacc ttggacgcac
     8941 acggtcgctt gtgtttcgtc caatgctttc tctgggcgga gggtgcaatg tggcccacca
     9001 atgatgatgg ggatgtccgg tttatgttgt gtaatcaatt gaattaaggt ttttacttca
     9061 ttcaacgagg ggctcaggac ggacattccc acaacatcag actggtgtaa tgtggttatg
     9121 agtctctgtt catcgaaggg ttctgctgaa aaatcaagga tcgttacgct gtctccctcg
     9181 ttctgaagca ttgttgcggc gtacagaaga ccaaggggtg gtgccagtcc atagaacaca
     9241 cgagaagtgc gtataaaaaa tgattctggg ggtgggtaga tcaataaaaa cctcatatcc
     9301 gctcccttgt ttgaaaccaa aagcctctca catgcaccat gttttcttta aacgacatgt
     9361 tcgtaattaa atattctggt cgactgtttc gggaaataat gatctttggt tgcagagatg
     9421 atgga
//
LOCUS       DATMXJ010000015         6898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_47199, whole genome shotgun sequence.
ACCESSION   DATMXJ010000015 DATMXJ010000000
VERSION     DATMXJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47199"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VN377_00555"
     CDS             <1..803
                     /locus_tag="VN377_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HWR26906.1"
                     /translation="YMWLAVENSINALIAGGTAAGKTSTLNALCLFIPRESKIVSIEE
                     TREINLPHPNWIPGVVRSGFGEVVGNKVIGEIDLYDLMKAALRQRPEYILVGEIRGKE
                     AYVLFQAMATGHTTYSTVHADSAQSLIHRLEGKPIEIPRIMLQTLDIVIIQVISRVKN
                     KRARRCKQIIEIIDIDPTTKEILTNEVFRWDPIEDKFVYSGKSYILERIRAEKDISRE
                     GMISEIKNRTMLLDWMNKQNIREFKKVATIIAQYTDNPSEVMKKIRNE"
     gene            911..2551
                     /locus_tag="VN377_00560"
     CDS             911..2551
                     /locus_tag="VN377_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HWR26907.1"
                     /translation="MVAFSSHEVSIDEKNLVELECYPVLEPFVFIKILKDTTSLDKYY
                     KVIEPQLNDAEKESLNFIQDTLITSIDIRLDELDVQKVEAFLSDRIGYIIKDYNLMVD
                     DVLKNKILYYVKRDFVWYGQIDPLMRDSQIEDISCDGSGQPLFLFHRKYGSLKSNIQF
                     SDEDVLSKFVTMLAQKCGKHISIAEPMLDATMPDGSRIQMTLSKAVTAKGSTFTIRRF
                     KADPITATDLIAYKTLSSEMAAYLWLAVENGISALIAGGTAAGKTSTLNAISLFIPRE
                     SKIVSIEETREINLPHPNWIPGVVRSGFGEVINDKLAGEIDLYDLMKAALRQRPEYIL
                     VGEIRGKEAYVLFQAMATGHTTYSTFHADSAQSLIHRLEGKPIEIPRVMLQSLDIVTI
                     QIQAELGEKRVRRVKQIVEIVDIDPMTKEILTNEVFRWDPVQDQFFFSGKSYVMERIR
                     NQHGQSKEDMLDELSRRVEILEWMKDNNIRAFKDVARIISSYIDTPDEVMEKVRKANQ
                     TKQKEQEITVENAIMKKQDEVASEMPSDITINKKNNKK"
     gene            2573..3448
                     /locus_tag="VN377_00565"
     CDS             2573..3448
                     /locus_tag="VN377_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR26908.1"
                     /translation="MTLTKYQRMSYKWLGRFGERRANQHLQDALQSAHMEIRAGAYLS
                     YVFLSTIIGFLCSLIVIVTFVFILLPLAGVMLDNGLVIFFLFTPGFIGVILYAALLSL
                     PKSRAKKRKKKIDVNLAYAINFISAMASAGVTPTEIFKSLSKQDIYGEVKEESAWIYR
                     DVGLLGFDIISAIKKNIARTPSQRFKEFLQGMVVTVTSGGSLKTYFMHKADQYLWENR
                     NQQKQLLETLGIMAESYVTAAVAGVLLLLIVIPLMMIITGDWNSMFLYILILIIVPLI
                     HIGFAVVIRGMCQGA"
     gene            3451..4353
                     /locus_tag="VN377_00570"
     CDS             3451..4353
                     /locus_tag="VN377_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR26909.1"
                     /translation="MKKSKEAHKTSYLRNLMLTKNVTRLTVNIVSGVLVTVFVLLGIL
                     SMLGIFTGFGGFVDYFVFALLSGTGVYGMYESIHVRRIQKIDGIFPDFVRDLAESRRA
                     GMTFTKAILFSSKGEYGILTPEIKKISQQISWGGSVADALESFAQRVNTKSIRRTITL
                     IIEASNSGGNVADVLDVAAKDAREIKMLEAERRTNMASYVVVIYVGMFVFLAIVLILC
                     TSFIPAMVGSEGSQGMQGVMGGGSNASQEEISGVFYLACLVQAFGSALVAGVFEDGRY
                     TSSVKHIFIMVFTTWLMFKLLLGV"
     gene            4354..6861
                     /locus_tag="VN377_00575"
     CDS             4354..6861
                     /locus_tag="VN377_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HWR26910.1"
                     /translation="MKKNIIASPFFKKEHAVSEVVGTLILIVIAVSTFSAVSMIILNP
                     WLNFSDVEVPKVYLVGSIHNDEILIEHRGGIPLDTKTKITITIDGTADTFRVNDFNYW
                     FDQNADGLWTVGEQIVYPAGNLQGKQVSCVIIDIDKNFIIFDKIIQQGTTVFSPYIAV
                     LPPDEVSETSAILKMYYNFYNQSFFPSGFLNFTYGIFGGPYSSSPQVKPLSLDGWYGL
                     QLNGLLSGTQYEYWSWMNCSNETLFDGPLSFYTYQVTRGLWHFDEAFGSPIAADAMNP
                     TCDGNVYDAIFIPGGKINGSLNLSGKSNYVDVPHHPKFNLTNEITIETWLNVSKIGAQ
                     FPGNVSELSTKNITDILGNSCLEPDLIHITGTMYAIAYRDNSTTSVTTFRMNDDGVFL
                     GTIETKLIPVPHFYEPTITKIQNNIYAIVYGASDDQTEAKSHIVTLSLYGNGTIGDII
                     DTFDFPQYYGREPNIINIGSDIYAIALGGASFETYPTGYLVTVSIDALGNIGSALIDT
                     LKFPLSTSCSESSIVHITGDIYGITYNGYGATAGMGYVITVHVLNNGSIVEPLEETVQ
                     FGLPEDGLEPTMIQVINDIFAISYGADSNDHLRTGYIKTLSINSIGHVLNDSIDILPF
                     YTYLSPIDYNFETDILHIEGELYAISFTGGNNSNWKRGFLTTVAIGGTGNISDSALFI
                     YEFKDRSALGGSSALNLATHVDRLIVVYGSINSTERGFLTMEKIDLIGEEKSIIKKGD
                     AVALTVNYNLLTARMMIGNTTYTVSGTVSFDSWKKIDLTYGGGFLQLYIDDVIQTGGS
                     EPCTGSLRTNTDHLIFGGGLYGSIDEIKIFRGIYVPV"
BASE COUNT         2105 a         1302 c         1485 g         2006 t
ORIGIN      
        1 cctatatgtg gcttgctgtg gaaaacagca taaacgcact gattgctggt gggactgctg
       61 caggaaaaac ttcaacactc aatgcactct gtttattcat cccccgggaa tcaaagattg
      121 tctcaatcga agaaacaaga gaaattaatc tacctcatcc gaactggatc ccaggtgttg
      181 ttcgatctgg ctttggggaa gttgttggca ataaagtcat tggagaaatc gacttatacg
      241 atttaatgaa ggcggcactc agacaacgac ccgagtatat ccttgttggg gaaattcgag
      301 gcaaagaagc gtacgtgctc ttccaggcga tggcgactgg gcacaccacc tactcaacgg
      361 tccatgcgga ctctgcacag tctctcatcc accgtttgga gggaaaaccc atcgaaatcc
      421 cccgcatcat gcttcaaacc ttagacatcg tcatcataca ggtcatctct cgggtgaaaa
      481 acaaaagagc acgacgatgc aaacaaatca tcgaaataat cgatatcgat ccaacgacaa
      541 aggagattct aaccaatgaa gtgttccgct gggaccccat tgaagataaa tttgtgtact
      601 ccggaaaaag ctacattctt gaacggatca gagcagaaaa agacatcagc cgggaaggaa
      661 tgattagtga aattaagaat agaacgatgt tgttggattg gatgaataaa caaaacattc
      721 gagagtttaa gaaagtcgct accatcatcg ctcaatatac agataatcca tcagaagtca
      781 tgaaaaaaat taggaatgaa taatgataaa gaaaattgcg atctgtcctg agtgtcattc
      841 aaaaatttcc tgtgaaggtg atcctggtca aaaagtaatg gtgaaatgtc ctaaatgtgg
      901 aaaaaaaggt atggtagcat tttcgtccca tgaggtgtcc attgatgaaa aaaaccttgt
      961 cgagcttgaa tgctaccctg ttctggaacc atttgtcttt ataaaaatat taaaagacac
     1021 aacgtcactt gacaaatact ataaagtaat tgaacctcaa ctgaatgatg cagaaaaaga
     1081 atccctcaat tttatacaag atacgctgat aacctctatt gatattcgtt tagatgaact
     1141 tgatgttcaa aaagtagaag cgtttctttc agatcgtatc ggttacatca tcaaggacta
     1201 taatttaatg gtcgatgatg ttttgaaaaa taaaatcctt tattatgtta aaagggattt
     1261 cgtttggtac ggacagatag atccattgat gcgtgattca caaatcgaag atatttcttg
     1321 cgatgggtca ggtcagcctc tttttttatt tcatagaaaa tatggctctc tgaaaagtaa
     1381 cattcaattt tctgatgaag atgtattatc taaatttgta acaatgcttg cgcagaaatg
     1441 cggcaaacac atttcgattg ctgaaccaat gcttgatgca accatgcccg atggctctcg
     1501 tattcaaatg acgttaagca aagcagtaac cgcaaaagga tcaacattta ctatccgaag
     1561 atttaaggca gatccgatta ctgcaaccga tttaattgcc tataaaacac tgtcctctga
     1621 aatggctgcc tacctctggc ttgccgtcga aaacggcatt agtgcactca ttgcgggggg
     1681 gaccgctgct gggaaaacgt caaccctgaa tgctatttca ttgtttatcc ctcgtgaatc
     1741 aaaaatagta tctatcgagg agacacggga aatcaaccta ccacacccaa actggattcc
     1801 tggtgtggtt cgctccgggt ttggtgaagt gatcaacgat aaactagctg gtgaaattga
     1861 tttatacgat ctgatgaaag ctgccctgag acagagacct gaatacattc ttgtgggaga
     1921 aatccgaggg aaagaggcat acgtgttatt ccaggcgatg gccactggac ataccacgta
     1981 ttcgaccttt catgctgact cagcccaatc ccttatccat cgattagaag gaaaaccaat
     2041 tgaaatacct cgagtgatgc ttcagtcttt agatattgtc actatccaaa tccaagcaga
     2101 attgggagaa aaaagggttc gacgagtgaa acaaattgtc gaaattgtcg atatcgatcc
     2161 gatgacaaaa gaaatattga ccaacgaagt gttccgatgg gaccccgtcc aggatcaatt
     2221 ctttttttcg gggaagagct atgtgatgga acgtattcga aatcaacatg gacagagtaa
     2281 agaagacatg ttggatgaac tgagtcgacg cgttgagatt cttgaatgga tgaaggataa
     2341 caacattcgt gccttcaaag atgtcgcccg tatcatatcc tcatatatcg ataccccaga
     2401 tgaagtaatg gaaaaagtaa gaaaagcaaa tcaaacaaaa caaaaagaac aagaaatcac
     2461 ggttgaaaat gctataatga aaaaacaaga tgaagtggcg tcagaaatgc catctgacat
     2521 tacgataaat aaaaaaaata ataaaaaata agaagaaaac ggagggatac cgatgacact
     2581 tacaaaatat caacgaatga gttataaatg gttaggccga tttggagaac gaagagcgaa
     2641 tcaacacctc caagatgctc tgcaatctgc tcacatggag atccgtgctg gtgcctatct
     2701 gtcctatgta tttcttagta ccattatagg ttttctgtgt tcactgattg tcattgtgac
     2761 atttgtattt attcttctgc ctctcgcagg tgtgatgttg gacaacggat tggttatttt
     2821 tttcctattt acccctggat tcatcggtgt aatactctat gcggcgttac tctctttgcc
     2881 aaaaagcaga gcaaagaaac gcaagaagaa gatcgatgtg aacctcgcct atgcgatcaa
     2941 cttcatctct gcgatggctt ctgcgggtgt gacgccaacc gagattttta aaagcctttc
     3001 aaagcaagat atatatggag aggttaagga agaatctgcg tggatatacc gtgatgttgg
     3061 tctgcttgga tttgatatta taagtgcgat taagaaaaat atcgctcgta ctccatcaca
     3121 acgattcaag gaattcctcc aggggatggt ggtcactgtc acctcgggtg gttctttaaa
     3181 gacatatttt atgcataagg cagatcaata cttatgggaa aatcgaaacc aacagaaaca
     3241 gctccttgag acacttggta ttatggctga gagctatgtg accgctgctg ttgctggtgt
     3301 attgctcctc cttattgtta ttcctctgat gatgattatt actggagatt ggaattccat
     3361 gttcctttac attttgatct taatcattgt gcctcttatc cacattggat ttgcagtcgt
     3421 aattcgaggt atgtgccagg gtgcgtaatt atgaaaaaaa gtaaagaagc gcataaaaca
     3481 agttacctac gaaatctgat gcttacaaaa aatgtgacac gacttaccgt caacatcgtc
     3541 tctggtgtat tggtgaccgt ttttgtcctg cttggtattc tgtcgatgct tggtattttc
     3601 accggatttg gaggttttgt cgattatttt gtttttgctt tactatcggg aactggcgta
     3661 tatgggatgt acgaatcgat tcatgttcga agaatccaaa agattgatgg gatttttcct
     3721 gattttgtac gagatctcgc tgaatctcgt cgagctggga tgacatttac aaaagcgatt
     3781 cttttttcat caaaaggaga gtatggcatc ctgacaccag agataaagaa aatctcccag
     3841 cagatttcct ggggtggcag tgtggctgat gcgttagaat cttttgcgca gcgtgtaaac
     3901 acaaaatcga tccgacggac tattactctc attatagagg caagtaacag tggtgggaat
     3961 gttgctgatg tccttgatgt cgctgcaaag gatgcacgag aaattaaaat gttggaggcg
     4021 gaaagaagaa ccaatatggc ttcttatgtg gttgtcatct atgtcggtat gttcgtgttc
     4081 ctggcaatcg tgttgattct atgtaccagt tttattcctg ccatggtggg gagtgaaggt
     4141 tcacagggta tgcagggtgt catgggcgga ggaagtaatg cgtcccagga agagatttct
     4201 ggtgtttttt atcttgcatg tctggtacaa gcctttggat cagcattggt tgccggtgtc
     4261 tttgaagacg gtcgatatac atcatcggtc aaacatatct ttatcatggt ctttacgacc
     4321 tggttgatgt ttaaacttct cttaggggtt tagatgaaaa aaaacataat cgcatcccca
     4381 ttttttaaaa aagaacatgc agtatctgag gtagtcggta ctttaatcct cattgttata
     4441 gcggtgagta ctttttctgc agtctcgatg attattctga atccttggct aaatttttca
     4501 gatgtcgagg tccctaaagt gtacctggtc ggatctatcc ataatgatga aatcctcatt
     4561 gagcaccgag gcgggattcc cttagatacg aaaacaaaaa taaccataac aatcgatggg
     4621 actgcagata cattccgcgt gaatgatttt aattattggt ttgatcaaaa tgctgatggt
     4681 ctttggactg ttggcgaaca gatcgtctac cccgccggga atctgcaagg aaaacaagtc
     4741 tcctgcgtca tcatcgacat cgataagaat ttcataatat ttgataaaat catccaacaa
     4801 ggaacgactg tgttttctcc atatatcgct gtgttaccac cggatgaagt atcagagacc
     4861 tctgcgattc tcaaaatgta ttataatttt tataatcaga gtttttttcc atcaggattt
     4921 ctgaatttca cctatggtat ttttggagga ccgtatagct cctctcctca agtgaaacca
     4981 ctatctctcg atggatggta cggtctacaa ttaaatggac tgcttagtgg tactcaatat
     5041 gagtactggt cttggatgaa ttgtagcaat gaaaccttgt ttgatggtcc tttatctttt
     5101 tatacctatc aagtcacacg aggtttatgg cattttgatg aagcgttcgg atcacctata
     5161 gcagcagatg caatgaatcc aacttgtgac ggaaacgtct atgatgcaat atttataccg
     5221 gggggaaaga ttaacggatc tctcaatctc tccggaaaaa gcaattatgt tgatgtcccg
     5281 catcatccta agtttaatct aaccaatgag ataacaatcg aaacatggtt aaatgtatca
     5341 aaaatagggg cgcaatttcc tggtaatgtg tcagaattat caacaaaaaa catcactgat
     5401 attttgggaa attcctgttt agagcctgat ttgatacata taactggtac gatgtacgcg
     5461 atagcgtatc gtgataattc tacaacgtcg gtgacaacct ttcgaatgaa tgatgatggt
     5521 gtttttcttg gtacgattga aacaaaatta attccagttc cccacttcta tgagcctacg
     5581 ataactaaaa tccagaataa tatctatgcg atagtatatg gagcgtctga tgaccagact
     5641 gaggcaaaaa gtcatattgt gacgttatcg ttgtatggga atgggacgat tggtgatatt
     5701 atcgatacat ttgatttccc tcaatactat ggacgtgaac caaatattat caatatcggg
     5761 tctgatatct atgctatcgc tctcggtgga gcgtcttttg aaacatatcc aactggatac
     5821 ttggtcaccg tatcgataga tgctctggga aatattggat ctgccctcat cgacacactg
     5881 aagtttccat tatcaacaag ttgttcagag tcctccatag tacatatcac gggtgatatt
     5941 tatggtatca cctataacgg atatggagca accgcaggaa tgggatatgt tataactgtt
     6001 catgtcctaa ataatggatc tatcgttgaa cccttagaag agacagttca atttggattg
     6061 cccgaggatg gtttagaacc aaccatgatt caggtcataa acgatatttt tgcgatttcc
     6121 tatggtgccg attcgaatga tcatcttcga accggatata ttaaaacttt gagtattaac
     6181 tccataggtc atgtcctgaa tgactctatt gatattcttc cgttttatac ctatctttca
     6241 cccattgatt ataattttga gacagatata ttacacatag aaggcgaact gtatgccata
     6301 tcttttactg gtggtaacaa tagtaattgg aaacgcggtt ttctcacaac ggtcgcgata
     6361 ggaggaaccg gtaatatctc agattcagcg ttatttatct atgagttcaa agatcgttca
     6421 gctctcggtg gatccagtgc cctgaacctt gcaacacacg ttgataggtt aattgtggta
     6481 tacggttcaa tcaactcaac cgaaagagga tttttgacaa tggaaaaaat cgatttaatt
     6541 ggtgaagaaa aatccattat caaaaaagga gatgccgttg cactcacggt aaattataac
     6601 ttactgaccg ctcgtatgat gattggaaat acaacctata ctgtttcagg gactgtatcc
     6661 tttgatagct ggaaaaaaat tgatttgacg tacggtggag gatttttaca actctatatc
     6721 gatgacgtca tccaaaccgg tgggagtgaa ccgtgcacag ggtcgctgcg aacgaataca
     6781 gatcatctca tattcggagg gggtctgtat ggctcaattg atgaaataaa aattttcaga
     6841 ggaatctatg tccccgtata attctatctt tgccaagagg aactgaagag gtatacaa
//
LOCUS       DATMXJ010000016         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_62942, whole genome shotgun sequence.
ACCESSION   DATMXJ010000016 DATMXJ010000000
VERSION     DATMXJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62942"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..482
                     /locus_tag="VN377_00580"
     CDS             36..482
                     /locus_tag="VN377_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26911.1"
                     /translation="MKKILLIIGICLVLVAMPMTTASPLLKSTPLKNLLQHGRPLMTN
                     GSFTGVFAEKNETGYNTLGTIDGTYTEEHTFTGAWSLFDGSASGTIDGWYWGYIFYGQ
                     VHTNGINESQWFGGLYRVNTTDNSFEAVSIIFGNDNFMIRYAMGTI"
     gene            726..2219
                     /locus_tag="VN377_00585"
     CDS             726..2219
                     /locus_tag="VN377_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR26912.1"
                     /translation="MNHSQRAILYVGGSGPNNYTKIQDAVDNASSDDTIFVYQGMYHE
                     HVVLNKKVTLLGELRDQTIIDGSQTGNVIKITIDGVTVEQFTLQNAGIGVYIVHSSDQ
                     TIIQNNITNNWEGIGLLDSAYNMISGNIITHNGFEGINPVQTTFTVIQDNTIIDHLQG
                     ISLSESTGNIITGNNLYSNLRGIEIQQSSNNNKIFHNNFFSSEESNGYDTCSNTWDDG
                     YPSGGNYWDDYNGGDANHDGIGDTPYNIAGGGGNKDYYPLMVPCYHSPYLPSDPVPLN
                     GAVNIPIAPELSVFVFDPNGDTMNVSFYDASNDQVIGVDNNVGSSTRASITWNGLENL
                     TQYHWYAIADDGLHITQSETWIFTTGNGMNQPPGKPTINGTTSGTIGQTYAYTFVTTD
                     PETNSVLYYIDWGDGTNSGWIGPNQSGQQITVFHTWSKRGTYTIKAKAKDMQNAESDW
                     GTLSIKMPKSHAQMTFLFMHFLERLFERFPQGFLILQYLFNVYGLHR"
     gene            complement(2250..3164)
                     /locus_tag="VN377_00590"
     CDS             complement(2250..3164)
                     /locus_tag="VN377_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HWR26913.1"
                     /translation="MEPIVDMLQSEFETLQANFKTVSINHLKDLQTELTTWQHDGIIT
                     KKFYDQNYGQFLFHPPEMLQNARSIIIIGVPQKINKVNFIHNGTQYQTIIPPTYIYSE
                     IRATCKGILSNILGKKGYSVERAILPMKLLAVKSGLGKYGKNNLCYVDGMGSFTRLEA
                     FYTDYEFPTDDWGEKEIMDSCKNCSLCRNACPTHCIPNDRILIHADQCLTYFNENEED
                     FPSSISAQSHNALVGCLHCQTICPEDRKYLQFNTQTIDFTEEEISLILQKTPREGIPK
                     TLTKKLIDLDMDEYYPLLQRNITVLMNK"
     gene            3361..3765
                     /locus_tag="VN377_00595"
     CDS             3361..3765
                     /locus_tag="VN377_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26914.1"
                     /translation="MKDKIGVIVGLLVTILVLVTLAFYMMNAGTIEFTNIASLAVIII
                     LIAAAAYIIWDRAKNIRKGLPAKDERLININYKAGYYGFIAALFSPFASQFIADITLE
                     HELTGSQVTAVIVITSGMVFVISYLYMAWKGN"
     gene            3768..3965
                     /locus_tag="VN377_00600"
     CDS             3768..3965
                     /locus_tag="VN377_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWR26915.1"
                     /translation="MKTRIKELRARYDLTQEDLAKKVGVRRETILFIEKGNYNPSLQL
                     AHDIAKTLQTTIDDLFIFEEE"
     gene            complement(4017..4796)
                     /gene="zupT"
                     /locus_tag="VN377_00605"
     CDS             complement(4017..4796)
                     /gene="zupT"
                     /locus_tag="VN377_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016508837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HWR26916.1"
                     /translation="MELPQHIIFAFLLTLLAGLSTGIGSLIAYFIRKPKMIYLSFSLG
                     FSAGVMIYVSFVELLPQAVVQIGRFTGIVVFFLGILLIAFIDMVIPEARNPHHYKEMP
                     DEQTLKVDKALLRTGLFTALAIAIHNFPEGLTTFGTALSDERLGLIIALAIAIHNIPE
                     GISVSVPIFYATGDRKKAFQYSFLAGLAEPIGAVIGFLILLPFLSSMLLSSLLAFVAG
                     IMVYISIDELLPMAHRYGHSHTVILGFILGMALMAASLFIL"
     gene            complement(4806..5858)
                     /locus_tag="VN377_00610"
     CDS             complement(4806..5858)
                     /locus_tag="VN377_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR26917.1"
                     /translation="MNLYRGCTHNCVYCDGRAEGYYVEGEFGTDVSVKINALEVLRRE
                     LDPRRKRTPLKPGYVLVGGGVGDSYQPAEEKYQLTRKTLALLSEKKFPVHILTKSTLV
                     RRDMDLLQQINETSRAIVSFSFSSTNESISRIFEPGVPSPQKRLETIAFFKDHGLACG
                     MFLMPVIPFITDTKEILQKTIQDAKDVGVDFIILGGMTLKEGKQKEYFFNILQRYYQD
                     LMLEYQMIYKGSKWGEAIPDYYDQLHQTFFSLTNLYKIPARIPPELFKDILNENDLVI
                     VILEHLDYLLKLEGKRSPFGYAAYSLSQLQVPISTMQYQLQTIKGVGPTTEKIIQEII
                     KTGSSTYYKRLLKSDL"
     gene            complement(5961..6317)
                     /locus_tag="VN377_00615"
     CDS             complement(5961..6317)
                     /locus_tag="VN377_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR26918.1"
                     /translation="MDQTCTIYRTIDFISKKWTLLILLELYKTQGTKRYSELKHGLLD
                     ITPKILSIRLRELEKQGLITKTIDTDVFPIKCEYTLTDSGKDFIRIIRDVKKWSLQWK
                     HKNKECESQDCKHCEL"
     gene            6400..>6521
                     /locus_tag="VN377_00620"
     CDS             6400..>6521
                     /locus_tag="VN377_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR26919.1"
                     /translation="MTKRKIIKINEEQCNGCGACIPNCPEGAIQVIDGKARLIS"
BASE COUNT         2018 a         1280 c         1329 g         1894 t
ORIGIN      
        1 ttatagccat ttctaccttt ggaggttaat gaaaaatgaa aaaaatatta ttgattatag
       61 gaatatgttt ggtattagta gcaatgccga tgacaaccgc gtcaccattg ttgaaatcca
      121 cacccctgaa aaatctctta caacatggaa gaccattgat gaccaatggg agtttcacgg
      181 gagttttcgc agaaaaaaat gaaacaggat acaatacttt aggaacaatc gatggaacct
      241 atacagaaga gcacaccttc accggtgcct ggagcttatt tgatggcagt gcctcaggaa
      301 caatcgatgg ctggtactgg ggatatatct tctacggtca agtgcatacc aacggaatta
      361 atgagtcaca atggtttgga ggattatatc gggtaaatac gacagataat tccttcgaag
      421 cagtatcaat catctttggt aatgacaatt ttatgattcg atacgccatg ggaaccatct
      481 aagaaatttt aatcaatcat ctcttttttt tctttttatt ctccatccag ttatacacta
      541 acatataaat aatacaacag atatgatatc atattggggg aactaccata gataaaccgc
      601 tcattcagct acgatggatc acgtttggaa tactcatcgt catctgtctt tcaaccatta
      661 ttccgtcttt ttcaaatgca tcacacacaa aacagggacc atgggacgaa ccatatctct
      721 actcattgaa tcattctcag agagcaattc tttatgttgg cggctcaggg ccaaataatt
      781 atacaaaaat ccaagatgct gtcgataacg caagctctga cgatacgatt ttcgtatatc
      841 aggggatgta tcacgaacat gtggtcctga ataaaaaagt gactctgctt ggtgagcttc
      901 gtgatcagac gattatcgat ggatcccaaa ccgggaatgt aataaaaatt actatagatg
      961 gagttaccgt ggagcaattt actcttcaga atgcgggaat tggggtctat attgttcatt
     1021 catcagacca aactatcatc caaaacaata ttacaaataa ttgggagggg attggtcttt
     1081 tggattcagc atacaatatg atttcaggga atatcatcac ccataatggt ttcgagggca
     1141 ttaaccctgt gcaaacgacc ttcacggtta ttcaagataa tacgattatt gatcaccttc
     1201 aagggatctc tctttccgaa tcaacaggaa acatcattac cggaaacaac ctttacagca
     1261 atctgcgggg aatagaaata caacaatctt cgaataataa taagatattc cataataatt
     1321 tcttttcaag tgaagagagt aatgggtatg acacctgtag taacacctgg gatgatggat
     1381 atccaagtgg gggtaactat tgggatgatt acaatggtgg agatgcaaat catgatggga
     1441 taggcgatac tccttacaat atcgcagggg gtggtggtaa caaagattac tatccattaa
     1501 tggttccatg ttatcactca ccatatttac cgtctgatcc tgttcctctt aatggtgcgg
     1561 tgaatatccc gattgcccca gagctttccg ttttcgtgtt tgatccaaac ggagatacca
     1621 tgaatgtttc gttctatgat gcatcgaacg atcaagtgat tggcgtcgat aacaatgttg
     1681 gtagttcaac gagagcatct ataacatgga atggattaga aaatttgaca caatatcatt
     1741 ggtatgcgat tgcagatgat ggattgcaca tcacacaatc agaaacctgg atattcacca
     1801 cgggaaacgg gatgaaccaa cctccaggaa aaccaacaat caatggcaca acctcgggaa
     1861 ccatcggaca aacatatgca tatacttttg ttaccacaga ccctgaaaca aacagtgtat
     1921 tatactatat tgactggggt gacgggacga atagcggatg gataggtccg aatcaatctg
     1981 gtcaacaaat cacggtcttc catacatgga gtaaacgagg aacgtatact atcaaagcaa
     2041 aagcaaaaga tatgcagaac gctgagagtg attggggtac gcttagtata aaaatgccga
     2101 agagccatgc acagatgacg tttttattca tgcattttct cgaaagacta tttgaacgat
     2161 ttccacaagg atttctaatc ctacagtatc tctttaatgt atatggacta caccgataaa
     2221 tccgtgtggt gcttaaatta attccagcat catttattca tcaacacagt aatgtttctt
     2281 tgtaataatg gataatattc atccatatca aggtcaatta attttttcgt tagggtcttt
     2341 ggaatacctt ctcgaggtgt cttttgtaaa atcagcgaaa tttcttcctc agtgaaatct
     2401 atagtttgtg tattgaattg aagatatttc ctatcttcag gacagatggt ttgacagtgc
     2461 aagcatccca ccaacgcgtt atgagattgc gcagaaatcg atgaggggaa gtcttcttcg
     2521 ttttcattga agtaggtaag acactggtcc gcgtggatta aaattcgatc attggggatg
     2581 cagtgagtag gacaggcgtt acgacataag gaacaatttt tacaggagtc catgatttct
     2641 ttttcacccc aatcatcggt gggaaattca taatccgtgt aaaacgcttc caatcgggta
     2701 aaactcccca ttccatcgac ataacagaga ttattcttgc cgtattttcc taatccgctt
     2761 ttcacggcaa ggagtttcat tggtagtatt gcgcgttcga cggaatatcc tttttttcct
     2821 aaaatatttg ataagattcc tttacaagta gctcgtatct cagaatagat ataggttggt
     2881 gggatgattg tctggtattg tgttccgtta tgaataaaat tcactttgtt gattttttgt
     2941 gggacgccga tgattattat tgagcgagcg ttttgaagca tttcaggtgg atgaaaaagg
     3001 aattgaccat aattttggtc atagaatttt ttcgtaatga ttccgtcgtg ttgccatgtc
     3061 gttagttctg tttggaggtc ttttaaatgg ttaatagaaa ctgttttaaa atttgcctgt
     3121 agggtttcaa attctgattg tagcatatcg acgattggtt ccatattcat catctgattg
     3181 ccattcatgg tatgcattgt ttctttaaca gattttgctt attttatcga ggagttcgtg
     3241 aggagtttat aaggtgaaga gagaaactat aaatagtatc tgttacatat atataacata
     3301 atgttagaaa taacaaataa aaagttacta ctaacacaca aaaattaggg gagaaaaaaa
     3361 atgaaagaca aaataggagt aatagtaggg ctgttggtaa caatcttagt gctcgtaacc
     3421 ctcgcattct atatgatgaa tgcaggaaca atagaattta caaatatcgc atctttagca
     3481 gtaataatca ttttaattgc agcagcagcc tatatcatat gggaccgagc aaaaaacatt
     3541 agaaaaggat taccagcaaa agacgaacga ctcataaaca tcaactataa agcaggatac
     3601 tacggattta tcgcagcact cttcagcccc tttgccagcc aatttatcgc agatatcaca
     3661 ttagaacatg aactaacagg cagtcaagtc acagctgtta ttgttatcac ctcaggaatg
     3721 gtgtttgtca tatcgtatct gtacatggct tggaaaggga attaaccatg aagacccgga
     3781 tcaaagaact ccgagcacgc tacgacctta cccaggaaga tttagcaaaa aaagtaggag
     3841 tccgacggga aaccatcctc tttattgaaa aaggaaatta taacccttca ttgcaactcg
     3901 cacacgacat tgccaaaacc ttacagacaa caatcgatga tctattcata tttgaggaag
     3961 aataaaacca tttatccgaa cagcaacggc agtcgaaggg atgatcgtac cgtactttat
     4021 aggataaaaa gactcgcagc cataagagcc atccctaaga tgaacccaag aataacggta
     4081 tggctatgac catagcgatg tgccatgggg agaagctcat caatggagat gtataccatg
     4141 attcctgcga caaaggctaa taacgatgac agtaacattg acgataagaa cgggaggaga
     4201 ataagaaaac caatcacagc accgatgggt tctgctaatc ctgctaaaaa cgaatattgg
     4261 aacgccttct ttcgatcccc agtcgcataa aaaatcggta ccgagaccga aataccctca
     4321 gggatattat gaatcgctat cgcaagtgct atgatgagtc caagtcgctc atcactgagt
     4381 gctgttccaa acgtggtgag tccctcgggg aaattatgaa tcgcgatggc aagagctgta
     4441 aacaacccgg ttctcaacag tgccttatcc accttcaggg tctgttcatc cggcatctcc
     4501 ttgtaatgat gagggtttcg tgcctcaggg ataaccatat cgataaaggc gataaggagg
     4561 attcctagga agaacacgac aataccggtg aatcgtccga tctgaacgac tgcttgtggc
     4621 aacagttcaa caaaggaaac atagatcatc actccagcgg agaaccccaa tgagaaggaa
     4681 aggtaaatca tctttggttt cctaataaaa tacgcgataa gactccctat ccctgtcgaa
     4741 agaccggcta ggagagttag tagaaatgca aagatgatat gctgtggtaa ctccatttgt
     4801 gcctcttata aatcgctttt taataagcgt ttgtaatatg ttgagcttcc cgtttttata
     4861 atctcctgga taattttttc ggtcgtgggt ccaacgcctt ttatggtctg taattgatac
     4921 tgcatcgttg atatcgggac ctgtagttgt gacagcgaat acgctgcgta accaaacgga
     4981 gatcgtttcc cttcgagttt gagtaaataa tcaagatgct ccagtatcac gatgaccaaa
     5041 tcattctcat taagaatatc tttgaaaagt tcgggtggga tgcgcgcggg tatcttgtac
     5101 aggttcgtaa gcgaaaaaaa cgtctgatgc aattgatcat aataatcggg gattgcttct
     5161 ccccatttac tccctttgta tatcatttga tattcgagca tgaggtcttg ataatatctc
     5221 tgcaggatat tgaaaaaata ttccttttgt tttccttctt tcagcgtcat cccaccaagg
     5281 atgataaaat cgactccgac atctttggca tcctgtattg ttttctgcag tatctctttg
     5341 gtatcagtaa tgaaaggaat aacaggcatg agaaacatcc cacaggcaag cccatgatct
     5401 ttaaaaaaag caatggtctc aagacgcttt tgtggtgaag gaacccccgg ctcaaagatt
     5461 ctgctgatcg actcattagt ggaggaaaaa ctaaaactca cgatcgccct gcttgtctca
     5521 ttgatctgct gcaaaagatc catatctcgt cgtacgagtg ttgattttgt aagaatatga
     5581 acagggaatt ttttttcaga tagaagagca agtgttttcc gggttaattg gtatttctcc
     5641 tcggcaggtt ggtagctatc cccgacaccg cctccgacca acacatatcc tggtttcagt
     5701 ggggtacgtt ttcgccgtgg atctaactct cggcgtaaca cttccaaggc attgattttt
     5761 actgatacgt cggtgccaaa ctcaccctca acatagtatc cttccgctcg cccgtcacaa
     5821 tagacacagt tgtgagtgca acctcgatag aggttcatcc cgtatctcga aagaaaccat
     5881 gaatcgattt ttttatggtt acgaagaatc gattttgctt ctatttcttt gattgtcaga
     5941 gatattcacc ttttcgaaga ttacaattca caatgcttac aatcctgaga ttcacattcc
     6001 ttgtttttat gtttccattg caatgaccat ttcttaacat ctcttatgat gcggatgaaa
     6061 tcttttccac tatcagtcaa cgtatattca cattttattg gaaaaacatc ggtatcgata
     6121 gtctttgtga tcagtccttg tttttcaagt tctcggagtc tgattgaaag tatctttgga
     6181 gtgatatcca aaagaccgtg tttgagctct gagtatcgtt tcgtcccttg tgttttatag
     6241 agctctagga gaatcagcag cgtccatttc ttcgagatga aatcaatcgt tcggtagatg
     6301 gtacacgttt ggtccatggt atccttttag atactgtcac ctataaatac ctttgtatct
     6361 aatatatact aggtataaaa aagatactga gtgatcggca tgaccaaacg aaaaatcata
     6421 aaaatcaacg aagaacaatg caacggctgc ggagcatgca taccgaattg cccggaagga
     6481 gccatacaag tcatcgacgg aaaagctcga ctcatcagcg a
//
LOCUS       DATMXJ010000017         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10605, whole genome shotgun sequence.
ACCESSION   DATMXJ010000017 DATMXJ010000000
VERSION     DATMXJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10605"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VN377_00625"
     CDS             complement(<1..624)
                     /locus_tag="VN377_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HWR26920.1"
                     /translation="MQEIWTEKYRPKTLSELVGQENIIERLKAYATTKNVPHLIFAGP
                     PGTGKTTAALALAHEVFGEHHWSQNLNELNASDERGIGIIRGKIKDFARTAPIGSTFK
                     IIFLDEADSLTQEAQAALRRTIEKYTHICRFILSVNYSSKIIEPIQSRCAVFRFKPLT
                     AEDVKKFIRKIATKEHLEITPDGLEALIFIARGDMRRAVNVLQVGASS"
     gene            783..1271
                     /locus_tag="VN377_00630"
     CDS             783..1271
                     /locus_tag="VN377_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26921.1"
                     /translation="MKKYLPRTILKLCGFFLIANLPFLTISQLVGIDTFIDSFNHPSS
                     FLYLKNEKLHSLDAKAEYIILEQPTYQGFSIQEGDHILYHTNKESLQQNIVNQVKSDN
                     GITTYYTTSYTDDYDGPIYEQQIVGKIIGRSEDNIWNTLCLQLWDLSIDKLNAVALFS
                     DS"
     gene            1325..2665
                     /gene="gcvPA"
                     /locus_tag="VN377_00635"
     CDS             1325..2665
                     /gene="gcvPA"
                     /locus_tag="VN377_00635"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWR26922.1"
                     /translation="MQFIPNTSLKKSMLHDLQLHDINDLFSDIPKEIKIEKLRLPPSR
                     HQQDVEDQMRQLANQNKSFCEMPSFIGGGYKPHYIPAAVKSIISRSEFYTAYTPYQPE
                     TSQGFLQAIFEYQSMIAELTGMDVSNASLYDGATALGEAALMSMRITKKKTFVIPENI
                     SWEKKSILTNYTKGAGITIKEIAYDNKTGKIDLEDLKKTINTETAGFYLENPNFFGVF
                     EDTIEDIHSLLRNNQSLFIVGVDPISLGICKSPGEYGADIVIGEGKSLGNPLDFGGST
                     LGIFACKNEFLRQIPGRIIGMTKDHQGKRAFCMTMQTREQHIRREKATSNICTNEGLC
                     MLATATYLSWLGGKGLQELSERNFERGQHLQKQICAIPGFSRVFSGTHFNEFVIRHPD
                     AAKVQTHLLRHGIQGGLLLEKWYPKLKNCMLFGVTEAHSEESMTRLVSLLKEVP"
     gene            2665..>2925
                     /locus_tag="VN377_00640"
     CDS             2665..>2925
                     /locus_tag="VN377_00640"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWR26923.1"
                     /translation="MYHSAIWDEPLLNELHDTNKEKYTAKSVLPDSLQRKKLIDIPNI
                     DETQVMRHFTRLSQMNYGIESGIYPLGSCTMKYNPKICEEICR"
BASE COUNT          882 a          624 c          601 g          818 t
ORIGIN      
        1 gctggatgca cccacctgta atacgttcac cgctcgtcgc atgtctcctc gtgcgataaa
       61 aataagtgct tcaaggccat caggggtgat ctctaaatgt tcttttgtag caatctttcg
      121 aatgaatttt ttgacatcct ctgcggtgag tggtttgaag cggaagacag cgcacctgga
      181 ttgtatcggc tctattattt ttgatgaata gttcaccgaa aggataaatc gacagatatg
      241 agtgtatttt tctattgttc gtcgaagggc agcttgtgct tcttgcgtaa gggaatctgc
      301 ttcatcgagg aagataatct tgaaggttga tccaatgggt gctgtacgag caaaatcctt
      361 gatttttcca cgtatgatac cgatgcctcg ttcatcactt gcattgagct cattgaggtt
      421 ttgtgaccag tgatgttctc cgaagacttc gtgtgcaaga gccagtgccg cggtggtttt
      481 accggttcct ggaggcccag cgaatatcaa atggggaacg tttttcgtag ttgcatatgc
      541 tttaagccgt tcgataatat tttcttgacc gacgagttca gagagtgttt ttggccgata
      601 tttctctgtc cagatttctt gcatcacgtt tctgtaatga atgtcccttc ttaatgtttt
      661 tttatccttt gatttttctt cgaatctgta ttttgtcttt ttgacatttt tccagacagt
      721 atttatatag agatattgac agacgttagc gtgtaacaga agagcatgaa tgaggtgatg
      781 atatgaaaaa atatctacca cgaacaatac tcaaactctg tggattcttc ctcatcgcaa
      841 atctcccgtt tcttacgata tcacaactcg ttggtattga tacgttcatc gactctttca
      901 accatcctag ttcatttctt tacttaaaaa acgaaaaact gcatagcttg gatgccaaag
      961 cagaatacat catcctggaa caaccaacat atcaaggatt ttcaattcaa gaaggagatc
     1021 acatcctcta tcataccaat aaagaatcgt tacagcagaa tatcgtgaat caagtgaaat
     1081 ctgataatgg aattacaaca tactatacca ccagctacac agacgattac gatgggccta
     1141 tctatgaaca acaaattgtc ggaaaaatca ttggaagatc tgaggataat atctggaaca
     1201 cactctgttt acaactatgg gatctttcca tagataaact caatgctgtt gcgctttttt
     1261 ctgattcgta aattctaagg ataagcttct ttaacatctg atggttaccc ttgagggggt
     1321 acccatgcag tttataccca atacttcact aaaaaaaagc atgttacatg acctacaatt
     1381 gcatgacatc aacgaccttt tttcggacat cccaaaagag ataaaaattg aaaaactaag
     1441 acttccaccg agtcgtcatc aacaagatgt agaagaccag atgcgacagc tagccaatca
     1501 aaacaaatca ttctgtgaga tgccatcatt catcggcgga ggatataaac ctcattatat
     1561 tcccgcagcg gttaaatcca tcatatcacg ttcagaattc tacactgcat atactcccta
     1621 tcaaccagaa acatctcaag ggtttcttca ggcgatcttt gaataccaaa gcatgatcgc
     1681 agagcttaca gggatggatg tctccaacgc ctccttatat gatggcgcaa cagcacttgg
     1741 ggaagcagcc ttgatgagca tgcgtatcac gaagaaaaaa acatttgtca ttcctgaaaa
     1801 tatctcctgg gagaaaaaaa gtatccttac caattacacc aaaggagcag gtatcacgat
     1861 aaaggaaatc gcttatgaca ataaaacagg taaaattgac ctggaggacc tgaagaaaac
     1921 gatcaatact gagactgcag ggttttattt agagaaccct aacttttttg gtgtgttcga
     1981 agacaccatc gaagatattc actccttact tcggaacaat caatcattgt tcatcgtcgg
     2041 tgtcgacccg atttctcttg gcatctgtaa aagccctgga gagtacggag ccgatattgt
     2101 cattggtgaa ggaaaaagcc ttggcaatcc ccttgatttc ggtggttcaa ccttagggat
     2161 tttcgcctgt aaaaacgagt ttttacgaca aatcccgggt cgcatcattg gtatgacaaa
     2221 agaccatcag ggtaaacgcg ccttctgcat gacgatgcaa accagggaac aacatatccg
     2281 aagagagaaa gcaacaagca acatctgcac caacgaaggg ctctgcatgc ttgcgacagc
     2341 aacttatctg tcctggcttg gtggaaaagg attgcaggaa ctatctgaaa gaaactttga
     2401 acgtggtcaa cacctacaaa agcagatttg cgcgatccct ggcttttcaa gggtgttttc
     2461 tggaactcat ttcaatgagt ttgttatccg tcatcctgat gctgccaaag tacagacgca
     2521 tctcttacga catggaatac aaggtggtct cctgttagaa aaatggtacc cgaagctgaa
     2581 gaattgcatg ttgttcgggg tgacagaagc acatagcgag gaaagcatga cacgactggt
     2641 ttcacttctc aaggaggtac cctgatgtac catagtgcta tctgggatga accacttctc
     2701 aacgaactac acgatacaaa taaagaaaaa tacactgcga aatcggtgct tcctgattct
     2761 cttcaaagaa aaaaactcat tgacatcccg aatatcgatg aaacccaggt gatgcgacat
     2821 ttcacgcgtc tgagccaaat gaattacggg attgaatcag ggatctaccc attgggttcg
     2881 tgcaccatga agtacaaccc aaagatctgt gaggaaatct gtcgt
//
LOCUS       DATMXJ010000018         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10614, whole genome shotgun sequence.
ACCESSION   DATMXJ010000018 DATMXJ010000000
VERSION     DATMXJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10614"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3599)
                     /locus_tag="VN377_00645"
     CDS             complement(<1..>3599)
                     /locus_tag="VN377_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR26924.1"
                     /translation="SFDGVDDWVDCGNGASLNPANAMTLVAWYMPTVSWYGAGDDPII
                     DKPYTSLSGPTYQYHLGVCGDLYPVPNSLAVGFDISPGGVWTWTGSPPNTLAFNQWSF
                     IVGTYDGSSMKLYVNGQLISSAFINGSLTDYGESLRFAKYYNAFEEAVYLPGAIDEIR
                     IYNRALNQSDISYLFTSVGGGNQPPFAEAGGPYSGDAEGTITFDASGSYDPDGTITGY
                     RWDWTNDGTWDTGWLNNATIPHFYSSLGTYTVNLEVRDDDGSTDSDLATVIITTGETI
                     LPFYDDFENIANGDYPSANGWQNMYSGANAFVSTDAAYSGAKSFCLQAYSDWARTDYV
                     QINPPEKLSYECYIKVIDSSQGAAIGFSIDAGSYNPRFNCVRFVYDGNIYFTKDYFEG
                     PNTFIQTYSANEWYKVKVDLEYSTNKATIFINDVQKATDVDIYPRSFTHYQWGSVTLD
                     KFAFALDNFDGGDVSTVYFDDVRIEASGGNQRPIADFTWMPQDPNPNQTILFDASASS
                     DADGYLTLYEWDWYNTGLFFEAHTTPTATFSWSQAGIYHVTLRITDNASATDSITKTV
                     HVGNQPPNTPVTPFGPTLLTAGQFYTFTTGTTDPDGDQIQYLFDWDAAGSHDYNITPF
                     VPSGELVSLSHGWNYGGIYFITVRALDTNEYYSEWSDSLMVIASGVNHPPNTPAAPTG
                     STSLITGETGTFSISATDPDDDNVFYKVNWGDGTETDWLGPYSSGSSLSISHSWVNTG
                     VTPVNVKAKDLLGSESGWSAEHIVTIRPPNHKPNSLKNPYPTNEMMNVDINTDLQWTG
                     GDQDSGDTVTYEIRLGTSIPPRKIAQIGPFPSTQGQLSYDPGTLIPNKKYYWKIVAYD
                     NHEASTSSTLWEFTTGSAPNNPPSASIDYIYPNPVQIGLSVGFQGHGTDIDGTITEYS
                     WRSSIDGYLGWNAFISTSLLSSGTHQIYFKVKDNNGAWSQEASKTLIVKTAGNKPPST
                     PSIPSGSSSGYTGVSYSFSTMATDPEGDYIYYYFDFNGDLLPDNSDSVSGSSGTTVSC
                     SWKWDKPGTYDIRVYAKDSKGAQSYWSGVKQITIQTSMSLTITTDKSTYRLGDSIWIT
                     ASVTSNNKPLGVLTAKNFAVTINGAPYYIQEFRTGTSQGIYQLRINSPSASGQHTITL
                     KVTTSEGSISKEVGITLTEGSYKVLLILACPKDLWNEQNQRPIYNWYNGVRGSP"
BASE COUNT         1008 a          787 c          744 g         1060 t
ORIGIN      
        1 gggctaccac gaacaccatt ataccaatta tatataggtc tttgattctg ttcattccac
       61 agatcctttg ggcatgccag tattaataaa actttgtatg atccctctgt taatgttata
      121 ccaacctctt tagagataga cccctctgaa gtagttacct ttaatgtgat tgtatgttgt
      181 ccacttgcag atggtgaatt tattcttaat tgatatatcc cctgagatgt cccagttcta
      241 aattcctgaa tatagtaagg tgctccattt attgtaacag caaagttttt cgccgttaga
      301 acccctagag gtttattgtt gcttgtgacc gatgctgtaa tccatatcga atcaccaaga
      361 cgatacgtag atttgtctgt ggtaattgtc aaggacatcg aagtctgaat ggttatctgt
      421 tttactcctg accagtagct ttgtgctcct ttgctgtcct tggcatatac tcgtatatcg
      481 taggtacctg gtttatccca tttccaggaa caggagaccg ttgtaccaga tgatccacta
      541 actgaatctg aattgtcagg taggagatct ccattgaagt cgaagtaata ataaatataa
      601 tcaccttcag ggtccgttgc catagtcgaa aatgaatagg aaaccccagt ataccctgag
      661 gatgaacctg atggaatact cggtgtagag ggaggtttgt ttccagctgt ttttacaatt
      721 aaggtttttg acgcttcttg tgaccatgct ccgttgttgt cttttacctt gaaataaatt
      781 tgatgagtac cacttgaaag caatgacgtg ctaataaaag cgttccagcc aagatatcca
      841 tcgatgcttg atctccagct atattcagta atcgttccgt caatatctgt cccatgacct
      901 tgaaatccaa cacttaatcc tatttgtacg gggttcggat atatgtaatc aatagaagct
      961 gaaggaggat tatttggggc tgagccagtg gtaaactccc ataatgtact agaggtagat
     1021 gcctcgtgat tatcataagc tacgattttc cagtaatatt tcttgtttgg tatgagtgta
     1081 ccaggatcat aggagagttg cccttgtgtc gatgggaaag gtccaatttg tgctattttt
     1141 ctgggtggta ttgatgtccc gagacgtatt tcataggtaa cagtatcacc tgagtcttga
     1201 tcaccaccag tccattgtaa atcagtgttg atgtcaacgt tcatcatctc attagttgga
     1261 taggggtttt ttaatgaatt aggtttatga ttcgggggac gaatagttac tatgtgctca
     1321 gctgaccaac cactctctga ccctaagaga tcctttgctt tgacattcac cggggttact
     1381 ccagtattta cccaactatg agatatgctt agtgatgatc ctgaggaata tggtccaagc
     1441 cagtcagtct cggtaccgtc accccagttg accttataaa acacattatc gtcatcagga
     1501 tcagtagcgc ttatactaaa tgtaccagtt tctccagtaa tcaacgaagt agacccggtt
     1561 ggtgcagcgg gggtattcgg cggatgattc acaccactgg ctatcaccat taagctatct
     1621 gaccattcgc tataatactc atttgtatca agagctctta ccgtaataaa ataaattcca
     1681 ccgtaattcc atccatgaga gagactgacg agttctcctg aaggtacgaa aggggttatg
     1741 ttatagtcat gagatccagc tgcatcccaa tcgaacaggt attgtatttg atctccatca
     1801 ggatcggtgg tgcccgtggt gaatgtatag aattgcccag cggtcaacaa ggtcggacca
     1861 aatggtgtga caggagtatt tggaggttga tttcccacat gaaccgtttt cgttatcgaa
     1921 tctgttgcac tcgcattatc ggttatccgt aaagtcacgt gatagattcc tgcttgagac
     1981 caagagaagg ttgcggtggg agtagtatgg gcttcaaaaa agagtccggt gttgtaccaa
     2041 tcccattcat acaatgtgag atatccatca gcatcgctac tcgctgaggc atcgaacaat
     2101 atcgtttgat tagggtttgg atcttgcggc atccaggtga aatctgcgat agggcgttga
     2161 tttccaccac ttgcttcaat tcgaacatca tcgaaataaa cggtacttac gtctccacca
     2221 tcaaaattat ctaacgcaaa cgcaaactta tcaagggtta cagaacccca ttgataatgc
     2281 gtaaaagaac gaggataaat gtcaacgtcg gtcgccttct gaacatcatt gatgaatatc
     2341 gtggctttgt tcgtactata ctccaaatct acttttacct tataccattc gtttgcactg
     2401 tatgtttgaa tgaaggtatt cggtccttca aagtagtcct tcgtaaaata gatatttcca
     2461 tcgtaaacaa atcgcacaca gttgaaacga ggattataac taccagcatc aatcgaaaaa
     2521 ccaattgccg ccccctgtga agaatcaatc acctttatgt aacactcgta gctaagtttt
     2581 tctggagggt tgatctgtac gtaatccgtt cgagcccagt cagaatacgc ttgcaaacaa
     2641 aatgatttcg caccactata ggcagcatca gtggacacaa acgcattggc accactatac
     2701 atattctgcc agccgttcgc gctaggataa tcgccatttg cgatgttttc aaaatcatca
     2761 taaaacggta atatcgtctc acctgttgtt ataatgaccg ttgctaaatc tgaatcggta
     2821 gacccatcat cgtctcttac ttcaagattg accgtgtagg ttcccaagct cgaatagaaa
     2881 tggggtatag tagcgttgtt gagccagcca gtatcccatg ttccatcgtt tgtccaatcc
     2941 caccgatacc ccgttatcgt tccatctggg tcatagctcc ccgaggcatc aaatgtgata
     3001 gttccctctg catcacctga gtaagggcca ccagcctcgg caaacggggg ttgatttccg
     3061 cctccaacac tagtaaataa atatgatata tcagattggt tcaaagccct attatagata
     3121 cggatttcat cgattgctcc tggaagatag acagcctctt caaaagcatt gtaatacttt
     3181 gcaaaacgaa gtgattctcc atagtcagta agagagccat taataaaagc agaggaaatc
     3241 aattggccat taacatataa tttcatcgag gatccgtcgt aggttcctac gataaaagac
     3301 cactgattaa aggctaatgt gttaggtggg ctacctgtcc aggtccacac gcctccaggg
     3361 cttatatcaa aaccaactgc taaactattg ggcactggat agaggtctcc acagacacct
     3421 aaatgatact ggtacgttgg gccactaagc gaagtatacg gtttatctat tattggatca
     3481 tcgccagctc cataccagga aacagttggc atataccatg ctacgagtgt catagcattt
     3541 gcaggattga gactcgcacc attaccacaa tcgacccaat catccactcc atcgaaact
//
LOCUS       DATMXJ010000019         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68184, whole genome shotgun sequence.
ACCESSION   DATMXJ010000019 DATMXJ010000000
VERSION     DATMXJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68184"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VN377_00650"
     CDS             <1..750
                     /locus_tag="VN377_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWR26925.1"
                     /translation="KKEVCSTGIEELDMKMSGGIPLGSTVLVTGCSGSGKTTLCMQFL
                     FNGARKGERGVFFTITEPLFKLTKNLEGFAFYDKKLIESGMVNIIDLRIISERLGLDT
                     EKYTVEDAGALLDILKDIADELDVKRLVMDSITALCYRLQTREMIRDFIFKLGTSLAV
                     MKCTTLLTSEVPPQTFKFSQYEIEEFISDGILFLGDMDRKGDLIRTLQVIKMRGTDHS
                     RTKFALNISAQRGIELVPLLKSTEFEAFLKR"
     gene            762..1337
                     /locus_tag="VN377_00655"
     CDS             762..1337
                     /locus_tag="VN377_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26926.1"
                     /translation="MVDAVIEGIRERIAAAIQVNQSVGIQVPENRHGDLLEAIFDSMC
                     RNTHTTWTYVTVSKTFDYLSKTFKEGTKQTNIKFIDCISRAAGISDIASNCIYIESPV
                     MLEKIILEILNNFKGMKRDLDKYIVIDSLSALMIYNDPEIIREFMSLVMNRSRSENIH
                     VVSILVEEEMDSSKLIQLNDKIIVLRDSFID"
     gene            1350..1916
                     /locus_tag="VN377_00660"
     CDS             1350..1916
                     /locus_tag="VN377_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26927.1"
                     /translation="MNQTDITKKENIHSSWEDTPAQKEQLAEWQESAFQAALEKLRTG
                     HGNPDSVMRMGEAFGRGLFAQRIKEQSPDWTMKQWLQLIEKDVCEPLGTEFTFTKISP
                     DVATTFLNRNPLAQTSSEHTAASLFNFGVMRGLFLSAFPQGELVISELKNNGQPEFIF
                     KANASAKDKLERERVIHSFTFLKEEDGA"
     gene            1921..2721
                     /locus_tag="VN377_00665"
     CDS             1921..2721
                     /locus_tag="VN377_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HWR26928.1"
                     /translation="MPRESFINTTVQKKEGFIRTGIDKLDRLLEGGIPSGFTVVLLGS
                     PGSSTEILVKQIASCGSVTYITTEETKQEILDTINRFKWPTPDIDFVDISEKYYTNVL
                     LGEQKRVSIYEQRSKLKLKELIEIGSTAMPSTSTASEDFLAVLSNRTKNVPEKRIIII
                     NSLDFFLSQYNQEEVLRTIYAIKVNNLKNKGALLIVMTKGVHGDLFERKMEGLADAVL
                     ELDVIQKGSSFERYLSVKKMRNYAKKIGIARYVIDDSGFVLEMIERIM"
BASE COUNT          870 a          607 c          638 g          641 t
ORIGIN      
        1 aaaaaggaag tttgtagcac cggtatcgaa gaacttgata tgaaaatgtc aggaggaatt
       61 cctcttggca gcacggtcct tgtcacggga tgcagtggat caggaaagac aacgctctgt
      121 atgcagttcc tgttcaatgg cgccagaaaa ggagaacgcg gcgtgttttt caccatcaca
      181 gaacctctct ttaagctgac aaaaaacctc gagggattcg ctttctatga caagaaactc
      241 attgaatcag gcatggtcaa tatcatcgac ctacgaatca tcagtgaacg attaggctta
      301 gacacggaaa aatacaccgt ggaagacgca ggagcattgc tcgacatcct aaaagatatc
      361 gctgatgagc ttgacgtcaa acgcttagtg atggactcca tcacagcatt atgctatcga
      421 cttcagacca gggagatgat cagggatttt atctttaaac tcggcacatc ccttgcggtg
      481 atgaaatgca caacgttgct gacctcagaa gtcccacctc agacatttaa attctcccaa
      541 tatgagatcg aggagttcat ctccgatggc attctcttcc ttggggatat ggaccggaaa
      601 ggggatctca tacggacgtt gcaggtcatt aaaatgcgag ggacagacca ttcccgtaca
      661 aagtttgcat taaacatttc tgctcaacgt ggtatcgagt tagtaccatt gttgaaatca
      721 actgagtttg aagcattctt gaaacgatag gagaagaaaa aatggtagat gctgttattg
      781 aaggcataag ggagcgaatc gcggcagcaa tccaggtgaa ccaatccgtt gggatccagg
      841 tccctgagaa cagacatgga gatttattag aagcaatctt tgactccatg tgcaggaaca
      901 cccataccac ttggacgtac gtcacggtga gcaaaacctt cgattacctc tcaaagacgt
      961 tcaaagaggg gaccaagcag accaatatca aatttattga ttgcatctcc cgtgccgctg
     1021 gtatctcaga catcgcctcg aactgcatct atatcgaaag cccggtgatg ctagaaaaaa
     1081 taatcctgga aattctgaac aatttcaagg gaatgaaacg tgatttagac aaatacatcg
     1141 tcatcgactc cctcagcgca ttgatgatct acaatgaccc ggagatcatc agggagttca
     1201 tgtccttggt catgaaccgg agcagatcag agaacatcca tgtcgtctcg atactggttg
     1261 aagaggaaat ggactcaagc aaactcatac aactcaacga taagatcatt gtcttacgtg
     1321 attcattcat tgattagaga ccagaaatta tgaaccaaac tgatataaca aaaaaagaga
     1381 atattcacag ctcctgggaa gatacacccg ctcagaagga acagctagcg gaatggcagg
     1441 agagcgcgtt tcaggcggct ttggaaaaac tcaggaccgg acatggaaac ccagactcag
     1501 tcatgcggat gggggaagcg ttcggcagag gactgttcgc gcaacggata aaagaacaat
     1561 caccggactg gacgatgaaa caatggctcc agctgataga aaaggacgtc tgtgaaccac
     1621 tcggaactga atttaccttt acaaaaatct cacctgatgt cgcgacaacg tttctgaacc
     1681 gaaatccact tgcacagaca tcttccgaac acacagcggc ctcactcttc aatttcggag
     1741 tgatgcgtgg actctttctc agcgcgttcc cacaagggga acttgtgatc agtgaactga
     1801 aaaacaacgg acaacccgag ttcatcttca aagcaaacgc atcagctaaa gataaactag
     1861 aacgcgaacg agtcatccat tcatttacct ttttgaagga agaagacggc gcctgagaaa
     1921 atgccacgag aatcctttat caataccacg gttcaaaaaa aagaagggtt catccgtacc
     1981 gggattgata agttagaccg gttgcttgaa ggagggatcc ctagcggatt tactgttgtt
     2041 cttctcggca gcccagggag tagtactgag attcttgtca aacagatcgc ttcatgcggg
     2101 agcgtcacct acatcacgac ggaagaaaca aaacaagaaa tccttgatac gataaaccgg
     2161 tttaaatggc caacaccaga tattgatttt gttgacatct cagagaaata ctacacaaat
     2221 gtcttattag gtgaacagaa gcgggtcagt atctatgaac aacgatcaaa gctgaaactc
     2281 aaggagctca tcgagatagg atcaaccgcg atgccatcga catccaccgc cagcgaagac
     2341 ttcttagctg ttttgtctaa tcgaacaaaa aacgttcctg agaaacgaat tattatcatc
     2401 aactctcttg atttcttcct cagtcagtac aatcaagaag aggtcctaag aaccatctat
     2461 gcgatcaaag tcaataacct gaagaacaaa ggtgctctgc tcattgtgat gacaaaggga
     2521 gtgcatgggg atctatttga gcggaaaatg gaagggctcg ctgacgcggt ccttgagctg
     2581 gatgtgatcc agaaaggctc gtcttttgag cggtacctct cggtaaagaa aatgcggaat
     2641 tacgcaaaaa agatagggat cgcacggtac gtcattgatg atagcggttt tgtcctggaa
     2701 atgatcgaac ggatcatgta gaaaacgcta gcgtgtcctt atcgattggt caatta
//
LOCUS       DATMXJ010000020         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_47258, whole genome shotgun sequence.
ACCESSION   DATMXJ010000020 DATMXJ010000000
VERSION     DATMXJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47258"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="kae1"
                     /locus_tag="VN377_00670"
     CDS             <1..755
                     /gene="kae1"
                     /locus_tag="VN377_00670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HWR26929.1"
                     /translation="PGLGPCLRTAATAARALALTLDKPLMGVNHCVAHLEIGRGTVQG
                     CQDPVLLYVSGANTQVIGFAEGKYRVFGETLDVGIGNCLDKFGRTIDLGFPCGPKIEE
                     LAKKGTQYLALPYSIKGMDLAFSGLLTAAETYVRQGKRLENVCYSLQETTFAMLTEVT
                     ERAMAHTDKQEVLLGGGVASNKRLREMVQIMAQDRGAKFFVPSRDVCVDNGAMIAWLG
                     FLMYDSGVRMSLDDSLINQRFRTDMVEVTWRD"
     gene            748..1371
                     /locus_tag="VN377_00675"
     CDS             748..1371
                     /locus_tag="VN377_00675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HWR26930.1"
                     /translation="MTNKIIYRGAEAEIRLSKYMDKKVVQKQRVKKPYRIKEIDRQLI
                     SYRTKEEAKLIAEARSHGVSVPLLYDVDLLKGAITMEYLEGSRVKDIFNGLKESERRR
                     ICLKIGESIARLHNFDLIHGDITTSNMIFLDDRIYFIDFGLGSKTTEMEAKGVDLHVL
                     MEAFESTHSKYSHCFEFVLEGYIKHYRGDGAAVVEKIEEIVRRGRYR"
     gene            1368..1928
                     /locus_tag="VN377_00680"
     CDS             1368..1928
                     /locus_tag="VN377_00680"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HWR26931.1"
                     /translation="MKQIFFITSNQGKVLEATEKLRPLGFSVVQKDLGYPEVQTDSLE
                     EVALWGASYVQERFHDPFILEDAGLFVDALKGFPGVYSKYVFLTIGLAGILRLLEGIE
                     NRKAVFRSVYAYGEPGKKPIIIIGECKGIISTEEHGNHGFGYDPLFIPDGGEKTFGEM
                     TVEEKNQFSHRGKALQKLAIVLKDLK"
     gene            2092..2164
                     /locus_tag="VN377_00685"
     tRNA            2092..2164
                     /locus_tag="VN377_00685"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2126..2128,aa:His,seq:gtg)
     gene            2299..2850
                     /locus_tag="VN377_00690"
     CDS             2299..2850
                     /locus_tag="VN377_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWR26932.1"
                     /translation="MDQKPLIGVSILAVVLLVMGSSIPVFAYSTNEHRKQLIKQDSDA
                     EELLQIPYMIVHVKEIYGTVDEPQYRPLANVTVRVRDSLFGIFWEYTWSGTTGESGST
                     PIIWITNSIKYWVTILKDGYHTYGCSSSKIIFALDQWTIDVDFTMAEDGSPFIKQINQ
                     LPHNSLILHQIAMRLFIINEDNP"
     gene            2847..>3055
                     /locus_tag="VN377_00695"
     CDS             2847..>3055
                     /locus_tag="VN377_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26933.1"
                     /translation="MKRKWLAIGIILLFVGVTIAPTINFNTVKASTDDDLVEVTTQAS
                     GIKGYGDTTVKLTREQVNEIDMLFES"
BASE COUNT          879 a          546 c          768 g          862 t
ORIGIN      
        1 gtcctggtct tggaccatgt ttgcgaactg cagcgactgc agcccgtgca ctggcgctga
       61 ccttagataa acctttgatg ggggtgaatc attgtgttgc gcatctagaa atcggtcgtg
      121 gtactgtaca agggtgtcag gatcctgttc tattatatgt ctccggggcg aacacgcagg
      181 tgattgggtt tgcagaaggc aaataccgtg tcttcggaga aacgcttgat gttggtatcg
      241 ggaactgtct tgataaattc ggtagaacaa ttgatcttgg attcccttgt ggaccgaaga
      301 tcgaggagct tgcaaaaaaa gggacacagt atttagcact cccgtactct atcaaaggga
      361 tggatctggc gttttctggt cttttgactg ctgcggaaac ctatgttcga caagggaagc
      421 gtcttgagaa tgtctgttat tcgctgcagg agacgacctt tgcgatgttg acagaggtga
      481 cagagcgggc gatggctcat accgataaac aggaggtgct ccttgggggc ggtgtcgcat
      541 cgaataaacg acttcgtgag atggtgcaga tcatggcaca ggaccgtggt gctaagtttt
      601 ttgtcccctc tcgggacgtc tgtgtcgata atggtgcgat gatcgcttgg cttgggttct
      661 taatgtatga tagtggtgtt cggatgagtc ttgatgatag cctcattaac caacggttcc
      721 gtaccgatat ggtggaggtc acgtggcgtg actaacaaaa tcatttatcg tggtgctgaa
      781 gcagagatcc gtttgtcaaa atatatggat aaaaaagtcg tgcagaagca gcgtgtcaaa
      841 aaaccgtatc ggattaagga aatcgaccgt caacttattt cgtataggac gaaagaagag
      901 gcaaagttga tcgcagaagc acggagtcat ggcgtatcag ttcccctctt gtatgatgtt
      961 gatctcttga agggtgcgat aacgatggaa tatctcgaag ggagtcgcgt caaggatatc
     1021 ttcaatgggt taaaggagtc agaacgaagg agaatctgtc tgaagattgg agaaagcatc
     1081 gcacgattgc ataattttga cctcatccat ggggatatca ccacgtcgaa catgatcttt
     1141 ctagatgacc ggatttattt cattgatttt gggttgggca gcaagactac tgaaatggag
     1201 gcaaaaggag tagatttgca tgtgcttatg gaggcattcg aatccactca ttcgaaatac
     1261 tctcattgtt ttgagttcgt ccttgaagga tatataaaac attatcgagg agatggagcc
     1321 gctgttgtgg aaaaaattga ggagattgta cggcgaggca ggtatcgatg aaacaaattt
     1381 tttttatcac aagcaaccaa ggaaaggtgc ttgaggcgac agaaaaactt cgacctcttg
     1441 gtttttctgt ggttcaaaag gaccttgggt accctgaggt gcaaacggat tctcttgagg
     1501 aggtcgctct atggggtgcg tcgtatgtcc aagagaggtt ccacgatccc ttcatccttg
     1561 aagatgcagg tttattcgtt gatgcgctca aggggtttcc aggggtgtat tcaaagtatg
     1621 tcttcttgac cattggttta gcagggattc tccgtttact cgaaggaata gaaaatagaa
     1681 aagctgtctt ccggtcagta tatgcttatg gcgaaccagg gaaaaaacca ataattatca
     1741 tcggtgaatg caaaggaatc atttcgacgg aggaacatgg aaaccatgga tttggttacg
     1801 accctctgtt tattcctgat ggtggtgaaa aaactttcgg agaaatgaca gttgaggaaa
     1861 agaatcaatt ttcgcatcgg ggaaaagcac tgcaaaaact tgccatagta ttaaaagatt
     1921 taaaatagat tctattagtt cctcaatagt cagaagttat ttctgacttt acattctccg
     1981 atactattat ataaagattt caggttttgg ggctgaatag acaaaaaagt attcgagacc
     2041 acagtgtgaa ataataaata ttcgatgtga cctagctctc ttgattagat ggcccgagta
     2101 gtgtagcctg gtaacatatg ggactgtgga tcctctgtgc cgggttcaaa tcccggttcg
     2161 ggccctctgt tactttttag catggcaata attgtctaaa agatggttga atatcgaaac
     2221 cgtgtcaaag ctttcccacg ggaggatatt ctttttatag gagaacagtg atgtaaaata
     2281 tgataatagg gggaaagtat ggatcagaaa cctctgattg gagtaagtat cctggctgtg
     2341 gttcttcttg tgatgggttc tagcattccc gtttttgcgt attctacaaa tgaacatcgg
     2401 aaacaattga taaaacagga ttcagatgct gaggagttat tgcagatacc ttacatgata
     2461 gttcatgtga aagaaatcta tggcacagtt gacgaacctc agtatagacc tttagcaaat
     2521 gtgacagtca gggttagaga tagtctcttt ggtatctttt gggaatatac ttggtctgga
     2581 accactggcg aaagcggaag cacacctatc atctggataa caaactcaat aaaatattgg
     2641 gtaacgattt taaaagatgg ctaccatacc tatgggtgtt catcctcaaa aataatattt
     2701 gctttagacc aatggacaat tgatgttgat ttcactatgg ctgaggatgg aagcccattt
     2761 atcaaacaaa tcaatcaatt acctcataat tccttgatac ttcatcagat agcgatgaga
     2821 ttgtttataa ttaatgagga taatccatga aaaggaaatg gttggcaatt ggaatcattc
     2881 tcttgttcgt tggtgtgacc atagcaccaa ctatcaactt caacaccgtg aaagcatcca
     2941 ctgatgatga cttggtggaa gtcaccaccc aagcaagtgg catcaaagga tatggggata
     3001 ctacagtgaa actcaccagg gaacaagtta atgaaattga catgctattt gaatc
//
LOCUS       DATMXJ010000021         8890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10615, whole genome shotgun sequence.
ACCESSION   DATMXJ010000021 DATMXJ010000000
VERSION     DATMXJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10615"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /gene="mgtA"
                     /locus_tag="VN377_00700"
     CDS             <1..1535
                     /gene="mgtA"
                     /locus_tag="VN377_00700"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HWR26934.1"
                     /translation="TLDKIVLEKHCDVTGKEDEDVFRFAYINSFYQTGLKNLLDKAIL
                     KYEKLPMGQYTKIDEIPFDFSRKIMSVIVTMNDVHRIILKGAPEEMFPRCSKYEMDGK
                     ILEMNETILDEIKHEYDSLSLQGFRVLAIAYKDFVDKKEVYSRDDENNLIMKGYVAFL
                     DPPKPTVKKTIEVVHKLGIELKVLTGDNEYVTKNICSQVGLDIRDVYTGDMINKLTDE
                     ELKDVVETTTVFVRLSPLQKERIIHALHENNHIVGYLGDGINDAPALKAADVGISVDS
                     AVDIAKESADIILQKKSLLVLGDGVIEGRKTFGNIVKYIKMGSSSNFGNMISMTGASI
                     FLPFLPMMPIQILMNNFLYDMSQTSIPTDLVDEEYLKRPQRWNIHSIQRFMILIGPVS
                     SIFDFATFGVLLFIFHAQQPLFTTGWFLESLCTQTLVIHVIRTGKVPFIESWPSKFLI
                     LTSIVIITVGIILPFSPLAGVLDFVTPPPLYFLALFVIILVYLLTVQLVKSLVVKKYG
                     YD"
     gene            1928..2275
                     /locus_tag="VN377_00705"
     CDS             1928..2275
                     /locus_tag="VN377_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HWR26935.1"
                     /translation="MSDMIAYCGLNCAECNAFKATRAKDFEGKKQLAKKWTEDDKVEF
                     RPEDIDCDGCKSNRISGWCRKICKIRPCAEERKVKTCAHCDDYPCEKLKEFLLKEPIA
                     KKNLEEIWNTLHV"
     gene            complement(2567..4495)
                     /locus_tag="VN377_00710"
     CDS             complement(2567..4495)
                     /locus_tag="VN377_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWR26936.1"
                     /translation="MNKDIVMQTANTETHCAMCRLDFLGTGVCPSGKKHGFVAYWPQG
                     RIEIYRALKAGTLAPTQQLFDIADTCTLCGVCDKQCSFITNRRPMIVMKALKEYVNGL
                     EKHTIQKTPSDPVLKELQHIVGEQWATNDPVILTAYKKTILAPTADNHTYVVMPKTSK
                     EVSDIIKVANANNLPYLPRGNGTFLSVALQTLLAGPVGISQGIILDLSRMNTITLNND
                     STTATIQAGVTAFDLQKTAASQNKRILVGEAEAYLCANVLSFGIISTWGNTYGWGADN
                     YTDVELVDAKGTILHHTGDTLKNPYAANHGPTSLTLHPSNIVTSMTIKLHQKSTDEHA
                     MLLPFDTLEDAVDFSLDLARKNIGLSLAILSSKYFSDFICPTNDIAHSFEHIITKYFH
                     INYLVDIICDSKDRRYVEDHADVIIDEPMMKTLALSTPKLASMKDNVLLKKIAQEDNP
                     LKTLFQGPMKRILTEALDPSAEQIGAAFDKDLKDFFTTLYKKPEMTNPIWLHEFRILP
                     SRMLRQHMFMVRGGWMTAQKDVILKAYATLKEIGDKYHKEHALGFISFLDSGKIAFLE
                     YDYYYDHTKPEEYQNLNPAIVESLTKELTIDGYLPIEFSFHKGLYRKGQVFYPFPKGL
                     TTEELQQFGLMVQQIVGG"
     gene            4639..5310
                     /locus_tag="VN377_00715"
     CDS             4639..5310
                     /locus_tag="VN377_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26937.1"
                     /translation="MAKDNAGLCTFDPDAPCSTCGNKNEIFCKPDENKVVVSHLLEGA
                     FIIMAVLGTGLTSLLLGSLWPVLVFSVFSALFFLVIQPRITCSHCPYYAEDRLFLHCT
                     ENHFSPKIWRYHPEPIAWWEKTGTAVGFAFLGAFPLLVELYGIYVFWVSSPSLVSLLG
                     VVGVFVGTMLTLALFYVVFFLLYCPHCVNFSCVFNKVPEEYVQRYLERNPVIKQAWEQ
                     QNKKG"
     gene            complement(6096..6593)
                     /locus_tag="VN377_00720"
     CDS             complement(6096..6593)
                     /locus_tag="VN377_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWR26938.1"
                     /translation="MKIGIVYYSRTGNTRQVAKILEEKFKEKNAEVDLIEIEHAKKPG
                     FFTAGKAASNQQELPIVNTEFDMEKYDVILTGSPTWAGKPTPFLKTFMDKAKNIKGKK
                     VAVFGTGMSPIDKRGQFIEIMKNNFEQKGIKTVDPCLTILFKRGQMVDGEQNIDNFIN
                     TCLKN"
     gene            complement(6690..8192)
                     /locus_tag="VN377_00725"
     CDS             complement(6690..8192)
                     /locus_tag="VN377_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR26939.1"
                     /translation="MLLVCSGCMNISDDDANNNPKKDTLVWGVLSAESIYPLGVTDDN
                     FWTIIPNIFDGLVEFDENFRIVPALAVSWNNPDSLTWRFYLRQGVKFHNGYDFTAEDV
                     KYSFETFHGGFDLIVRDIIILDNYTIEFKTFEPNPGLLSRLAHTGIIYCKNVTGQPAD
                     QTLLGTGAYRLADYEMDNYTTLERFDQYWGEKPEIKTVVFKAMEDDEERLNALLSGTI
                     DIAEYNIDDKIDQISQEENITVVKFPPLSTYIIGFDMRENGSYGFPDGMNPTADVRVR
                     KAIYQAINITPLITGPFQGFAQPESQFISPYIFGYNPEIQRLPYNISSSRQLLAEAGY
                     EQGFDIVMDGITEGYEYNAENCYLIVQQLSKVGIHVMMNNLSTDEYNDKVVRERNTSM
                     YLVGWGTISADGGSMYDYFIRSVGEHLGSLNSGHYSNTEVDRLGAAASQEMNAEERLH
                     LLQEGFRIALVDDVMAVPLFSQELLVLTAKNIELRPRADLRSVVKDIRIT"
     gene            8480..>8890
                     /locus_tag="VN377_00730"
     CDS             8480..>8890
                     /locus_tag="VN377_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26940.1"
                     /translation="MKKSQLNVILTAGAVTVLLFSGCVSGIVSTFQSTTTDSSRQKNI
                     VTNNGARITCYVGGILHTRTISYEAGKYLNELFSALVQANANDPCGKGTQMLKVRFIE
                     LLANLGLVSPHLSVQDVCSLIEPPWLHSALSLRRS"
BASE COUNT         2439 a         1765 c         1929 g         2757 t
ORIGIN      
        1 tgaccttgga taaaatcgtc ctggaaaaac attgcgatgt cacagggaaa gaagacgaag
       61 acgtattcag attcgcttat attaatagtt tttatcaaac cggattaaaa aatctgttag
      121 ataaagctat tttaaaatac gaaaaattac ccatgggcca gtatacgaaa attgatgaaa
      181 taccatttga tttctcccgg aaaatcatgt cagttatcgt aacaatgaat gatgtccatc
      241 gaatcatctt aaagggagca cctgaagaga tgttcccccg atgttcgaaa tatgagatgg
      301 atggtaaaat cctggagatg aatgagacta ttcttgatga gataaaacat gaatatgata
      361 gtctcagcct ccaaggattc cgtgttctcg caatcgcgta caaggatttt gtggataaaa
      421 aagaggtata ctcacgagat gatgaaaata atttaatcat gaaaggttac gtagcgtttc
      481 tagatccacc aaaacccacg gtgaaaaaaa ccattgaagt ggttcacaaa ctgggcattg
      541 aactcaaggt attaacaggt gataacgagt acgtcacgaa gaatatctgc agtcaagtcg
      601 gtttggatat tagagatgtg tacaccggtg acatgatcaa taaattaacc gatgaagaac
      661 tcaaagatgt cgtagaaaca accacagtgt ttgttcgact gtcgccctta cagaaggaac
      721 gaatcatcca tgcacttcac gaaaataatc atatcgttgg atacctaggt gatgggatca
      781 atgatgctcc tgcactcaaa gcagctgatg tcgggatttc tgtcgatagt gcggtggata
      841 ttgcgaagga atccgctgat atcatcctgc agaaaaagag tctcctagtc cttggtgatg
      901 gagtgattga gggtaggaaa acgtttggaa acatcgtgaa atacatcaag atgggatcaa
      961 gttccaactt tgggaacatg atcagtatga cgggagcaag catctttttg ccgtttctac
     1021 ctatgatgcc tatccagata ctaatgaata attttctcta tgacatgtca caaacaagta
     1081 ttcccactga tttagttgat gaagaatatc ttaaacgacc acaacgatgg aatatccact
     1141 ccattcaacg ctttatgatc ctcattggac cggtgagttc gatatttgac tttgccacct
     1201 ttggcgtcct cttgtttatt ttccatgcac agcagccatt attcactacc ggttggtttt
     1261 tagaatcact ctgcacacaa acattagtga tccatgttat tcgtacaggg aaggtaccat
     1321 ttatcgaaag ttggccgagt aagtttttaa ttctcacttc gattgtgatc atcacggtgg
     1381 gaattattct accgttttct cctcttgcag gagtattaga ttttgttact cccccaccac
     1441 tctatttcct tgcgcttttt gtaattattc tcgtgtatct cttaaccgtt caacttgtga
     1501 agagtctggt tgtgaaaaag tacggatatg attagaatac atactgataa tatctcaaat
     1561 ttttttgagt aacgaagcag tgtataaaat gttaaaacac gattttagct gttttttcca
     1621 gtgggatttg aacctatgta ccgtttatcc cttcagcagc ttggccatct ttttttcgca
     1681 tataaaaaat atttcttctt ataagaaata gaaaaaaaga gattttgatc ctcggaacct
     1741 ttcaacaaaa tcgttatttt ttcgaacaaa aacactctag cctaaaggta tattataata
     1801 ggagaacagt gttatataat tatacaaagg gggaaggcta tgaataaaaa tactctgatt
     1861 ggagtcgata gcagcctgca gtcacggtat attaggcaac attggaaagg gagagaggtg
     1921 aaagaacatg tctgatatga tcgcctattg cggattgaat tgtgctgagt gtaacgcgtt
     1981 caaagcgact cgggccaaag attttgaagg aaaaaaacag ttggcaaaaa aatggacaga
     2041 agatgacaag gttgagttca gaccagaaga cattgactgt gacgggtgca agtctaatag
     2101 aatttctggt tggtgccgga aaatctgcaa gataagacct tgtgctgagg aaagaaaagt
     2161 caaaacctgt gcccactgcg acgattatcc gtgcgaaaaa ctgaaagaat ttcttttaaa
     2221 agaaccgata gcgaaaaaga atctggaaga gatctggaac acactccacg tctgatagga
     2281 gcgttaatac gatacgttga ctcaaaatct aaggagagta gagttgaatt tacatactaa
     2341 aaaccgagtc ccaaaaataa ccatttttta tcttttttcc ttgtagtcat ccagactttt
     2401 tttccgtatt ccttcgccgg cttatctctt acttttctcc atacaaatgg gttttaaggc
     2461 ctaagaatta tgcaagtcta ctacaaccgt cgaaaatcaa aagtttcctt cggaatctta
     2521 taaaaaccac cctactcgaa tttcatcatt tgctggattt ccagggttat cctcccacga
     2581 tttgctgaac catgagtcca aattgctgga gctcttccgt ggtcagtccc tttgggaacg
     2641 ggtagaatac ctgtcctttt cgatagagtc ccttatggaa agaaaattcg atgggaaggt
     2701 atccgtcgat ggtgagttct tttgtcaatg attcgacgat agcagggttg aggttttggt
     2761 attcttcggg tttggtatga tcataatagt agtcgtattc gaggaacgcg atttttcctg
     2821 agtcgagaaa actgatgaac ccgagtgcgt gttctttgtg gtatttgtca ccaatttctt
     2881 tcagagtcgc atatgctttg aggatgacgt ctttttgtgc tgtcatccat ccgcctcgca
     2941 ccatgaacat gtgttgacgc agcatacgac tggggaggat acggaactca tgcaaccaga
     3001 tgggattggt catttctggt ttcttgtaca gcgttgtgaa aaaatccttt aggtctttgt
     3061 caaatgctgc tccgatttgt tctgctgagg ggtccaacgc ctcggtgagt atgcgtttca
     3121 ttggtccttg aaaaagggtt ttcagagggt tgtcttcttg tgctattttt ttgagtaaca
     3181 cgttgtcttt catagaggca agcttcgggg tactcagcgc gagtgtcttc atcatgggtt
     3241 catcgatgat gacgtcagcg tggtcttcaa cgtagcgtct atccttgctg tcgcagatga
     3301 tgtccacgag gtagttgata tggaaatatt ttgtgatgat gtgttcgaag gaatgtgcga
     3361 tgtcgtttgt cgggcagatg aaatctgaga aatatttact ggaaaggatc gcaagtgaca
     3421 gtccgatgtt tttcctggca aggtctaaag agaaatccac ggcgtcttca agggtgtcaa
     3481 agggcagaag catggcgtgt tcatcagtgg atttttggtg cagttttatc gtcatgctgg
     3541 taacgatatt tgaaggatgc agggtcaggc tggtgggtcc atggtttgcg gcgtacgggt
     3601 ttttgagtgt atcaccggta tgatgcagga tggttccttt cgcatccaca agctctacgt
     3661 ctgtgtagtt gtccgcaccc caaccgtacg tgtttcccca agtggagatg atcccgaagg
     3721 aaagaacgtt ggcacacagg tatgcctctg cttctccaac gaggattctt ttgttctgtg
     3781 aagcagctgt tttttggaga tcgaacgcgg tgacccctgc ttggatcgtc gcagtcgttg
     3841 aatcattgtt aagggtgatg gtgttcatgc gagacagatc gaggatgatt ccttgtgaga
     3901 ttccgaccgg tccggctaaa agagtttgca aggctacgga gagaaatgtc ccatttcccc
     3961 tagggagata gggtagatta ttggcatttg ctaccttgat gatgtcgctt acctccttgc
     4021 ttgttttcgg catgacgaca tacgtgtggt tgtccgcggt tggagcaagt atggtttttt
     4081 tgtatgcggt gagaatcaca gggtcgtttg tcgcccattg ctccccgacg atgtgttgca
     4141 gttcttttag gacagggtct gagggtgttt tttgaatggt gtgtttctcc aatccgttta
     4201 catattcctt caatgctttc atgacgatca tcggtcttct gttcgtaata aaggagcatt
     4261 gtttgtcgca taccccgcag agggtgcagg tgtcagcgat atcgaagagt tgttgtgttg
     4321 gtgccagggt gccggccttt agcgcccggt aaatttcaat tcgcccttga ggccaatacg
     4381 cgacaaatcc atgctttttc cctgatggac aaactccagt tcctagaaaa tcaagtctgc
     4441 acatcgcgca atgcgtctct gtgtttgctg tctgcatcac aatgtcttta ttcataggat
     4501 tacgctccct caaacgtctt ttgtttttta tggcggaatt acgctcgtcg atttaaagtt
     4561 attctccata tttcctattt atttgattgg tgtgtcgtag tcggttttta tatacccgtt
     4621 gtgtttccct ttttgcgtat ggcaaaagac aatgcaggtc tttgtacctt tgatcctgat
     4681 gcaccgtgtt cgacctgtgg gaataagaat gagatctttt gtaaaccaga tgagaataaa
     4741 gttgttgttt ctcatctgct ggagggggct tttatcatca tggcggtgct ggggactggg
     4801 ttaaccagtc tgcttcttgg aagtctgtgg ccggtgcttg tgttttccgt gttctcagcg
     4861 ttgttttttt tggtgattca gcctcgtatc acctgcagtc attgtccgta ctatgcggag
     4921 gaccggttgt tcctgcactg tacggagaat catttttctc cgaagatctg gcggtatcat
     4981 cctgagccga tcgcttggtg ggagaaaact ggtaccgctg ttggttttgc gtttcttggg
     5041 gcgttcccct tgcttgtaga gctgtacggc atctatgtgt tttgggtgag tagtccgagt
     5101 cttgtgtcat tgcttggtgt tgttggtgtg tttgtcggga ccatgcttac gttggcgttg
     5161 ttttatgtgg tgtttttctt gttatattgt ccgcattgtg tgaatttctc ctgtgtgttt
     5221 aacaaggtcc cggaagagta tgtacaacgg tatctcgaaa gaaaccctgt gataaaacag
     5281 gcatgggaac aacagaacaa gaaaggatga tacaagttca tgaggtgttc accataaaaa
     5341 tttctgtcaa aggcaactgg gaggctttcg actgatttca tttcaaaaaa tcattatttc
     5401 attatgaaat agaatctgat tttaatgtcc aggggttcaa atctcttccc ccgaattttc
     5461 atttataaat tttttatgga tctcttcttc ttattgtatc tattttggta aaagccaaaa
     5521 cagcctaaca tgacaaaata acagagtata agcatatttc catagaattt cgatacaagg
     5581 aaaccgtacc agttcaaggc aacacttcca atttgaagca ggataagata tatcattaaa
     5641 ccaacaggcc aacgatcagg atgatgttga ggtttctttt tagaaatgaa acgataccag
     5701 agatcataga gcaaataaac aatgattgga aacccagtaa tcaaacggtc tgtatcgctt
     5761 tggttgagaa ggttcaatat ccataaagca acaaacggga ctagcaagaa gctaaatatc
     5821 ggagataact tcaattccag tgtatcgcgt tcatacgcac gtaatacata tactaaacac
     5881 aagaaaatgt agtagataat tgcagaaaaa acaacaaggt tgtctatcag gatatcattc
     5941 ataggataat gcccttcatt caattgtaca gtaatacttt ctataaataa gattgggttc
     6001 catcatctcc tcatgcttgt cattatatcg aagttccgaa aaaaaaatct ccaggacatc
     6061 tcagcaatca gatggcagag atgcccattc attctttagt ttttaagaca ggtattgata
     6121 aaattatcaa tgttctgttc tccgtctacc atttgtcctc ttttaaaaag tatggtaagg
     6181 caaggatcaa ctgttttgat tcctttctgc tcgaaattat ttttcataat ctcaatgaat
     6241 tggcctcttt tatcaatcgg gctcatacca gtcccaaaaa ccgccacctt cttcccctta
     6301 atatttttcg ctttgtccat gaaagtcttc aggaacggtg ttggttttcc tgcccatgtt
     6361 ggggaaccag tgagtatcac atcatatttt tccatatcaa attcagtgtt cacgattggt
     6421 aattcctgtt ggttcgatgc tgccttccct gcggtaaaaa aaccaggttt ttttgcatgt
     6481 tcgatttcga taagatcgac ttccgcgttc ttttccttaa atttttcttc taatattttt
     6541 gcgacttgtc ttgtgttacc tgttcttgaa taatatacta ttccaatctt catacatcta
     6601 tcaactcgag taaggcaaaa cgcacatcgt ttataatttt tttcctagat tttgagaatc
     6661 tcagcggcga ctatctgctt ttgaatccct caggtaattc tgatatcttt tacgacgctc
     6721 ctgagatccg ctcgcggtct taactcgata tttttcgcgg ttaggacgag caattcttgg
     6781 gagaacaacg gaacagccat gacatcatcg accaacgcaa tcctgaatcc ttcctgtaac
     6841 aggtgtaatc gttcttcagc attcatctcc tgagaagcag cagcacctaa ccgatctacc
     6901 tctgtgttcg aataatgtcc gctatttaac gaaccaagat gctccccgac agatcgaata
     6961 aaataatcat acatagaacc cccatcagcc gagatcgttc cccatccaac cagatacatc
     7021 gatgtgttcc tctccctcac aactttgtca ttatactcat cggttgagag gttattcatc
     7081 atgacatgga ttccgacctt ggatagctgc tggacgatga ggtaacaatt ttctgcatta
     7141 tactcatacc cttcggtgat accatccatg acaatatcga acccttgttc atatcctgcc
     7201 tctgccaaga gttgccggga cgagctgatg ttatatggca atcgttgtat ctctggattg
     7261 tacccgaaga tataagggga gatgaactgg gattcaggct gtgcaaagcc ttgaaacggg
     7321 ccagtaatca aaggagtaat attgattgct tgatagattg cttttctcac acgaacatca
     7381 gcggtaggat tcatgccgtc gggaaatcca tagcttccat tctctctcat atcaaatccg
     7441 atgatatagg tggataatgg tgggaacttt accacggtta tattctcctc ttgtgagatt
     7501 tgatcaatct tgtcatcaat attatattct gcaatatcaa tggttccgga gagaagtgca
     7561 ttcaatcgtt cttcatcatc ttccattgct ttaaacacaa ccgtcttaat ctcaggtttc
     7621 tccccccagt attgatcaaa ccgctctaac gtcgtataat tatccatctc gtagtcagcc
     7681 aatcgatatg cccctgttcc gagcagtgtc tggtccgcag gttgtccggt aacgtttttg
     7741 caatagatga tacctgtgtg tgccaatcga gaaagaagac caggatttgg ttcaaatgtt
     7801 ttgaattcga tcgtataatt atcaaggatg atgatatccc tcacgatcaa atcaaaacca
     7861 ccatggaacg tctcaaagga gtatttgacg tcctctgcgg taaaatcata tccgttgtgg
     7921 aattttactc cctgacggag gtaaaaacgc cacgtcagac tatcagggtt attccatgag
     7981 acagccaaag ctggtacaat acggaaattc tcatcgaatt cgacaagacc atcgaagata
     8041 ttgggaataa ttgtccagaa attatcatca gtgacaccca gtggatatat actctctgca
     8101 gacagaacac cccatacaag agtatccttt tttggattgt tattcgcatc gtcgtctgat
     8161 atattcatac atcctgagca aaccaacagc atgagtatga gtgtgatgaa gagtattttt
     8221 ttatttgacc ggatttcagg catatacttc cgacatcaat gtattcaatt gatagagaat
     8281 aaggagcagg cttttaacat atacgctgta gcctcttcgt ctgtttctct tacaatatat
     8341 ttctgataaa aattattata aacaccgtcc ggtgaaaata aaatgattca aggatgaaga
     8401 gaatatattt atatagagaa ctatctctat attcgagaag aaaacggttt ataaatcata
     8461 ttgagagagg gagaaaagta tgaaaaaatc tcaattaaat gttattttaa ccgctggtgc
     8521 tgtcaccgtt ctattgttca gtgggtgcgt gtctgggatc gtgagcactt ttcaatcaac
     8581 cacaacagat tcctcgcgac aaaaaaatat tgttacgaat aatggtgcaa gaatcacttg
     8641 ctatgttggt gggattctgc atacccggac tatttcgtac gaggcaggta aatatttgaa
     8701 tgagttgttc tcagcgttgg ttcaggcgaa cgccaatgat ccctgtggca agggtactca
     8761 gatgttgaag gttcgtttta tcgagttact tgcgaatctc ggtttagtct cccctcatct
     8821 ttctgtccag gatgtctgtt ccctaattga gccgccatgg ttacactccg cgttatccct
     8881 gcgaaggagc
//
LOCUS       DATMXJ010000022         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68194, whole genome shotgun sequence.
ACCESSION   DATMXJ010000022 DATMXJ010000000
VERSION     DATMXJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68194"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VN377_00735"
     CDS             <1..252
                     /locus_tag="VN377_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26941.1"
                     /translation="SLYQTMHLAHSKHFIIWLLTSFTPSYHLDKTRLTTDQLEKLYEL
                     HTKREKLILKLKKMKIEIVELTPQMDGRNILEHVRGKHA"
     gene            249..758
                     /locus_tag="VN377_00740"
     CDS             249..758
                     /locus_tag="VN377_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26942.1"
                     /translation="MKIQTHLPYVLLSYLFVVLLLQIEMTLQNIVLASVPFIVLLIGL
                     VRRKKYLGMTGMFLFYLIALLQLRMFTINENILLILELVFFIIPSLLLLQIILQLDHT
                     ELVFKIGGTRPLLIAGILIVLISGVFILLVSIFPDNLVLLVESTQQQIIFLGVLTLIC
                     GTPLLMRKK"
     gene            complement(776..1762)
                     /locus_tag="VN377_00745"
     CDS             complement(776..1762)
                     /locus_tag="VN377_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWR26943.1"
                     /translation="MNIGPSSGIDFKAVRNTCELIKSEVGKVVVGYEDVVDDLLVCLI
                     TQNHMLMLGIPGIAKTTLAKTFSAVTGLSCSRVQFTQDLLPSDIIGHHYFDQKTSSFQ
                     LRLGPIFTEIMLADEINRSPPKTQSALIESMQEKQVTIEGTTLPLPSPFLVIATKNPI
                     ETEGVYPLPEAQLDRFLYRVSMDYLKPELELEMLRHKNNNTSTSVRVVGREAVKSLIG
                     LHHKIYTDQSILTYILSILNETRHTDKVVLGGSPRAGEHLLYASKAYALIHGKEYVIP
                     DDVKAVAPKVLGHRLLLSAESELEGLSTEKVIDDILRAVEVPDIVESVVLTR"
     gene            complement(1759..2859)
                     /locus_tag="VN377_00750"
     CDS             complement(1759..2859)
                     /locus_tag="VN377_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWR26944.1"
                     /translation="MNSAMKGVYQFLILGGIGVLLFVLALATPMVQNTSEFSMYNAGW
                     NGCSNIAVKTYTEGKFQPTFTVDTNELTLSPKSFVEYPLDPKNATILIVGPRSEFSSR
                     EADYLQGFLKKGGMVLLADDFGTGNDLLVQLNTTARFTGDLLLDLSFEKKPSFVTIFD
                     FPKPSHPVLRNVSHILLNYATSISTGKNTSVLAVSSQLSWRDTNLNGKEDAGEENGPF
                     PVLATERYGNGTIVLLSDPSVLINSMKNQLDNAVFRENLLQYLFLDRGTVIIDESHRQ
                     DPLLLHIFYTIPANLGAMEKVAIVLLVIGAFLVGFTKIPSYIVQKVIQLIVKEEKPAE
                     KTSAEKMIEELLTRHPSWSGKKLKGLVRELKE"
     gene            complement(2859..>3293)
                     /locus_tag="VN377_00755"
     CDS             complement(2859..>3293)
                     /locus_tag="VN377_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWR26945.1"
                     /translation="ALSVSLVLFLLRKKQAPAPTKKSNLTSLSSPAPLSQEVSKTCMN
                     LQEFFADTSTKGKDAFKTSVISRYQSLLTFLSTQGLVLLPSYTHLDIRKTMLSEGFPN
                     TETEIVTETFERALYSPYPLSEQEVTVFDHHLQRLLQKQGMT"
BASE COUNT          886 a          818 c          698 g          891 t
ORIGIN      
        1 tccctgtacc agacgatgca tcttgcgcat tcgaaacatt tcatcatctg gcttctcacg
       61 tcgttcacac catcgtatca tcttgacaag acacgcctca ccacggatca gctggagaaa
      121 ctctacgagc ttcataccaa acgggaaaaa ctcatcctta aactaaaaaa aatgaaaatc
      181 gaaatcgtgg agctcacccc gcagatggat ggaagaaaca tccttgagca cgtccgaggg
      241 aaacacgcat gaagatacag acacacctcc cgtatgttct tctcagttac ctgttcgtcg
      301 tccttttgct tcaaattgag atgacactcc aaaacatcgt ccttgcatct gtccctttca
      361 tcgtcctttt gattggactc gtacgtagga agaaatatct tgggatgaca ggcatgttcc
      421 tgttctatct catcgctttg ctgcagctcc gcatgtttac gatcaacgaa aatatcctgt
      481 tgattcttga gcttgtgttc ttcatcatcc ccagcctcct gctcctccag atcatccttc
      541 agcttgacca tacagagctt gtctttaaga tcggagggac cagaccgctc ctcatcgcag
      601 gaatcctaat cgttcttatc tccggggtgt ttatcctcct cgtctctatc ttccccgaca
      661 atctcgttct tcttgtggaa tccacgcaac agcaaatcat attcctcgga gtacttacgc
      721 tcatctgcgg tacaccatta ttgatgagaa aaaaataaag gaaaaaaaat ttgtcttagc
      781 gtgtgagtac tacagactcc acgatgtcag ggacttccac tgcacgtaaa atatcatcaa
      841 taaccttttc tgtagacaat ccttcaagct cagactcagc agaaagcagc agacgatgcc
      901 cgagcacttt tggcgccaca gcttttacat catcagggat gacgtactcc ttcccatgga
      961 taagcgcata tgctttggaa gcgtagagaa gatgttcacc agcacgggga ctcccaccga
     1021 gcaccacctt atcggtatgt ctggtctcat tcaggatact taaaatatag gtaaggatgc
     1081 tctgatcggt gtagatctta tgatgcaatc cgataaggct ctttacggct tctctgccaa
     1141 ccaccctcac cgaggttgag gtgttgttgt ttttgtgtcg caacatctcc agttccaact
     1201 caggcttgag ataatccatg gacacccgat acaggaaccg gtccaactgc gcctccggaa
     1261 gaggatacac accctcagtc tcgataggat tcttcgtcgc aattactaaa aacgggctgg
     1321 ggagaggaag cgttgttccc tcgatagtca cctgtttttc ctgcatggac tcaatcaacg
     1381 cggattgcgt cttgggcgga gagcggttaa tctcatcagc aagcatgatt tcagtaaaaa
     1441 tcggccctaa cctcaactga aacgagctcg tcttctgatc aaaataatga tgcccgatga
     1501 tatcagaagg aagcagatcc tgggtaaact gtaccctgct gcacgacaga cccgtgacag
     1561 cggaaaacgt ctttgctagc gtcgtttttg cgatacctgg gatacccagc ataagcatat
     1621 ggttctgggt gatcaaacac acgagtaaat catccacgac atcctcatag ccgacgacaa
     1681 ccttccccac ctcggacttt atcagttcac aggtgtttcg cacagcttta aaatcaatac
     1741 cagatgatgg acctatattc attctttcaa ctcccgtaca agacccttga gcttcttccc
     1801 actccaagat ggatgtcttg taagcaactc ctcaatcatt ttctcagcag atgtcttctc
     1861 tgctggtttt tcctctttaa cgatcaactg tataactttt tgtactatat atgatggtat
     1921 tttcgtaaat ccaactagga atgcaccaat cactaataaa actatagcga ccttctccat
     1981 cgcacctagg ttcgcaggta tcgtatagaa gatatgtaac agcaaaggat cctgccgatg
     2041 actctcatcg atgatcaccg tacccctgtc caaaaagagg tactggagta gattttcgcg
     2101 gaacaccgcg ttatctaact ggtttttcat cgagttgata agaacagaag gatctgaaag
     2161 caaaacgatc gtcccattgc catatcgttc ggtagcgagc acgggaaacg ggccgttttc
     2221 ctcccctgca tcctcctttc cattcaggtt cgtatcacgc caactcagct gcgaagacac
     2281 agcgagcacg gatgtattct tcccagttga gatactcgtc gcataattca gcaggatatg
     2341 agaaacgttc ctcaggacag gatgagacgg ttttgggaaa tcaaaaatcg tgacaaaact
     2401 cggttttttt tcaaacgata aatcaaggag aagatcacca gtaaaacgcg ctgtcgtatt
     2461 cagctgtacg agcagatcat ttcccgtacc aaaatcatca gcgagcagca ccatgccacc
     2521 ctttttgaga aaaccttgga ggtagtctgc ttcacgtgaa gaaaactcag agcgaggacc
     2581 aacaataaga atcgttgcgt tctttggatc aagaggatat tcgacaaatg atttcggaga
     2641 tagggttaac tcgtttgtat cgacggtgaa cgttggctga aatttccctt cagtgtaggt
     2701 ttttaccgcg atgttgctgc agccgttcca ccctgcgtta tacatgctga actccgaagt
     2761 attttgaacc atgggtgtcg cgagcgcaag gacaaaaaga agcaccccta tgcctccgag
     2821 gatcaagaat tgatagaccc ctttcatggc tgaattcatt aggtcatccc ctgtttttgt
     2881 aacaatcttt gcagatggtg gtcaaatacg gtcacctctt gttcactcag aggatatggt
     2941 gaatacagtg cacgttcgaa cgtctctgtc acaatctcgg tttctgtgtt tgggaatcct
     3001 tctgaaagca tggtcttacg gatgtcaaga tgtgtataac tcggcaataa cactagtcct
     3061 tgtgtagaca aaaacgttag caatgactgg taccgggaga tcaccgaggt cttgaatgca
     3121 tcctttccct tcgtcgaggt atctgcgaaa aactcttgta ggttcatgca tgtttttgat
     3181 acttcctgag acaacggagc aggggaggaa aggcttgtta gattgctttt tttcgtcgga
     3241 gcaggtgctt gttttttcct gagtaaaaaa agaacgagcg atacgctaag tgc
//
LOCUS       DATMXJ010000023         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36904, whole genome shotgun sequence.
ACCESSION   DATMXJ010000023 DATMXJ010000000
VERSION     DATMXJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36904"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1132
                     /locus_tag="VN377_00760"
     CDS             38..1132
                     /locus_tag="VN377_00760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWR26946.1"
                     /translation="MKLTLRFTTMVVGFLLLLVGLSSSGITAMILIGPYPQHSQLDSI
                     TISWKTETPTTQNEVRWGRSPALGDITVEKDIFPTSFHSVKIRGLLANQKYYYTVVSD
                     GTESPLYTFWTAFERNETIRFVVNGDTRGGWDNWLSTLLVSQAMEKEQPAFVFNTGDL
                     VDEGKNADDWIDFFTASPFVHNSTFYPVLGNHENYSHLYFSFFSLPSNERWYSFDNGP
                     VHFIALDSNLRSRYRLAQYFWLLYELQTDRQPFTIVMFHHPPYSSGSEHGNSSWLQRF
                     WVPVFEHFQVDIVLNGHDHDYERSIVNGITYIVTGGGGAPLYDVGHSPWTIYSEKTFH
                     YCLLTVDSSTLLFEAKKPDGFVFDSFVLSR"
     gene            1210..2088
                     /locus_tag="VN377_00765"
     CDS             1210..2088
                     /locus_tag="VN377_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWR26947.1"
                     /translation="MNKNILIGLLLVLVSLVWAGSFIVVEIVTKEMDPVDLGFLRFLV
                     ATPLMFLLVVLRKKPLLLPKKEFPWLVVLGLTGVTLLYLFQFLGIHYTNAPTASVLIN
                     TNVIFIAILSSLFLHETLTRKRVGGIVLSFIGVFVIMFSDLSTQKMTFDNLFFIGGIL
                     MLLSAFCWALYSLVGKHLLQRYDGFVITTYAFGLGTLFYLPFVFLHIGPVLRQTSLNG
                     WLAILYLALTCSLFGYLGWYYALKYVDASKAAVFLNFIPLFTILMSFFLGTSFTWFFL
                     LGAGLIIYGVYLTQRT"
     gene            2107..>2964
                     /locus_tag="VN377_00770"
     CDS             2107..>2964
                     /locus_tag="VN377_00770"
                     /inference="COORDINATES: protein motif:HMM:NF016842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMD3-related protein"
                     /protein_id="HWR26948.1"
                     /translation="MFCVECGKDEPIFRNGVCLSCYLKHTQFSKGPTIMDITICPRCS
                     SYKHKNTWVQESFDDILKQHIKDAFSISPELKDVTIQTQCDEQVRILHCMVSISGFLE
                     GQPITEQHPLTIRIRRNTCDVCSREAGGYYEAIIQIRAEKRTFPKSELKILRSTVESL
                     VTQLQKSGKRGLYITDFDENRQGLDFFLSEKATAFSIAKKIQEQFGGEFKQSASTAGM
                     KDSKQVFRMTYLVRIPAYRKGDFFVCDHSFFLIVSLHENKVRAIDLSTWTEKVIAGKD
                     SQPSKIFGGV"
BASE COUNT          727 a          640 c          687 g          910 t
ORIGIN      
        1 ctcatatata aggatatatt tgaaagattg aggatctatg aaactaacct tacgattcac
       61 tacaatggtt gtaggtttcc ttctgttgct cgtcggatta agcagcagtg gtattactgc
      121 aatgattctc attggtccgt atccccaaca ttctcaactc gactcgatta ccatttcatg
      181 gaagaccgaa accccaacca ctcaaaacga ggtgcgttgg gggcgaagtc cagcgctagg
      241 agatatcacc gtagaaaaag atatttttcc aacatcattt cattcggtga aaatcagagg
      301 gttactagca aatcagaagt actattatac cgttgtttct gatgggacag aaagtcccct
      361 ctatacattt tggacagcgt tcgagcggaa tgagacgatt cgatttgtag tcaatggcga
      421 tacgcgtgga ggctgggata actggctgag taccttactc gtgtctcaag cgatggagaa
      481 agaacaacca gcatttgttt tcaataccgg ggatcttgtc gatgaaggga aaaacgcaga
      541 cgattggatt gattttttca ctgcatcacc ttttgtccat aacagcacgt tctatcctgt
      601 gcttgggaat cacgagaact acagccatct gtatttttcc ttcttttcac ttccttccaa
      661 tgaacgctgg tattcatttg acaatggacc agtgcatttc attgctcttg actcgaacct
      721 gcgctctcgg tatcgattgg cccagtattt ctggcttctg tatgagttac agactgacag
      781 acaaccgttc accatcgtca tgttccatca cccgccgtat agctcaggat ccgaacatgg
      841 gaattcaagc tggctgcaaa ggttctgggt tcctgtcttt gaacattttc aagtagacat
      901 cgtgttgaat ggacatgacc atgactacga acgcagtatc gtcaatggta tcacttatat
      961 cgtcacgggt ggaggaggtg ctccgctgta tgatgttggg catagcccct ggacgatcta
     1021 ttcagaaaaa acgtttcatt attgtctgct taccgtggat tcatcaacgt tactctttga
     1081 ggcaaaaaaa ccagatggat ttgtttttga ttctttcgtt ctttctagat aaagcgttct
     1141 ggtttccaga gcgcggcaga agttactcac gatatcgttt tataaccttg tgcctgttta
     1201 tcggtgctca tgaacaagaa tatcctcatt gggttgcttc tcgttcttgt gtcattggtc
     1261 tgggctggat cgtttattgt tgtggaaatc gtgacaaagg agatggaccc tgttgattta
     1321 gggttcctgc ggtttctggt ggctactcct ttgatgtttt tgctcgtggt ccttcggaag
     1381 aaaccgttac tgcttccaaa aaaggaattc ccctggttgg tggtcctggg attgaccggg
     1441 gtgaccctgt tgtatttgtt ccagttcctt gggattcatt atacgaacgc tcctaccgct
     1501 tctgtgctca ttaatacgaa tgtgattttc attgcgattc tttctagtct cttcctgcat
     1561 gaaacgttga cacgaaaacg ggtgggcggc attgttttgt cgttcatcgg ggtttttgtc
     1621 atcatgtttt ctgatctttc aacgcaaaag atgacctttg ataacctttt ttttatcggt
     1681 gggatcctga tgttgttatc agcattctgc tgggcactgt attccttagt tgggaaacat
     1741 cttctgcaga gatatgatgg gtttgtgatt accacctatg cgttcggcct tggtacattg
     1801 ttctatctcc cctttgtttt cttgcatatc ggaccggttc ttcgacaaac ctcgttgaac
     1861 gggtggctag cgattctcta ccttgcgttg acgtgctctt tgtttgggta tctgggttgg
     1921 tactatgcgt taaaatatgt tgacgcgagt aaagcagcag tgtttttgaa cttcatccct
     1981 ctttttacga ttctgatgtc gtttttcctt gggacgagtt ttacctggtt tttccttctg
     2041 ggggcaggct taataatcta tggtgtatat ctcactcaaa gaacctaaca ggaatggatt
     2101 acaatcatgt tttgtgtcga atgtgggaaa gacgaaccaa tctttcgaaa cggtgtctgt
     2161 ctgagttgct atctgaagca tacgcaattt tccaaaggac caacaatcat ggatatcacg
     2221 atttgccctc gttgttcctc ctataaacac aagaacacct gggtgcaaga atcctttgat
     2281 gacatcctga aacagcatat caaggatgca ttttcgatta gtcctgaact gaaagatgtc
     2341 acgatacaaa cccagtgtga cgaacaggtt cgcatcctcc attgtatggt gtccatctct
     2401 ggttttttag aaggccaacc gatcacggag caacacccac tgacaatacg aatcagacgg
     2461 aacacctgtg acgtgtgctc tcgagaggct ggagggtact acgaggcaat catccagata
     2521 cgggcggaga aacgcacatt tccgaagagt gagctaaaga tccttcgctc tacggttgaa
     2581 tccctggtca cgcagttaca aaagagcggg aaacgagggt tatatatcac tgactttgat
     2641 gaaaatcgtc aaggccttga tttcttcctc agcgaaaaag caactgcgtt ttcgatagcg
     2701 aaaaaaatcc aggagcagtt cggcggggag tttaaacaat ccgcgtccac cgcagggatg
     2761 aaagacagca aacaggtctt tcgcatgacc tatctcgtcc ggatcccagc atatcggaag
     2821 ggcgatttct tcgtatgtga ccattcgttt ttcttgattg tttcacttca tgaaaataag
     2881 gtccgtgcca ttgatctttc cacttggact gagaaggtga ttgctggaaa agacagtcag
     2941 ccgtcgaaga tatttggtgg cgtg
//
LOCUS       DATMXJ010000024         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10693, whole genome shotgun sequence.
ACCESSION   DATMXJ010000024 DATMXJ010000000
VERSION     DATMXJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10693"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VN377_00775"
     CDS             <1..1456
                     /locus_tag="VN377_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWR26949.1"
                     /translation="LCMEEQKKHHHKIQEELSKIKKECEVKKISYEKLLTTTRQLEEK
                     KGKFERKQRLENDIQRIEKTLRERKETLEKLNETLLRFKTLDKELQDFEKQQEKNTSV
                     IQTSIKKKEQNLTLSTRLRADIKELTEKKHAIEKIGPQAKCPTCDRVLGDQQKKLIVA
                     FTKDIEKKNHESRRFIDEQKELQLEFDRSSREKQALQKKNMFLRSRVVEREKSLNTIR
                     HTTQEMERENQELEHKKKDLKTLGVADFDEKQYRLIRTRVNEAYTLYQGSLTKLYETN
                     EKQQKIAVELKEQEGEKKLIDQQIKTYEKNIEDQQRLEKKLENERTEAQHQNMLSEVM
                     ESFRTYLIAQIRPMLSAHASELFDQLTDGKYSQIELDEDYDLMVYDHGAPYSIERFSG
                     GEEDLANLCIRLAISEIITERAGSVFQFVILDEIFGSQDQIRKQNIIRTLNGFSSKFK
                     QIFLITHVEDIKHFTEHTMMISEDETGISSIKIE"
     gene            complement(1482..2438)
                     /locus_tag="VN377_00780"
     CDS             complement(1482..2438)
                     /locus_tag="VN377_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26950.1"
                     /translation="MTEEKVGGIGVKGVKDSFMEQLQEASTELGSVKSSFSKGMEDLA
                     KIQSMLSFEGLNKMDSMMRSFEDRLSEAERRRDEAAEGARRYSMELEKEKERLIKLWD
                     AYKNQEESLATQEKRVAELDERLRDTEQMHMQFERDATVRIQTLTQKLDERAQAVQQM
                     DDLKQQAMRFDTIRTQMETNVDRMRGEISTKDEVIRGLERQVEELRGFEQFADFKPKF
                     EEVSMEFEKEKERLTKLFRLYEETESENKQLKEELRGWQGWFDTNEELFTKLFSSVEH
                     LKQQHTTAPTSETTETDIEIPPSLQDQPTPIEKPKRRLRFRK"
     gene            2619..3284
                     /locus_tag="VN377_00785"
     CDS             2619..3284
                     /locus_tag="VN377_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HWR26951.1"
                     /translation="MGLLERFRESRFSKMMFKIFGKNHAKIGIYGPPNVGKTTLANRI
                     LRDWTGDIMGSVSEVPHETRRAKWKNNIRIDNGGSSLTMDLVDTPGIATKIDFKEFMN
                     MYGMTKDDSRVRAKEATEGVIEAIRWLDDIDGVVLVMDATQDPFTQVNVTIIGNLEAR
                     NLPILIAANKIDAENASPATLKSAFPQHPVIPISALTGYNTENLYTMMIKEFGKKRHK
                     QRR"
     gene            3290..3667
                     /locus_tag="VN377_00790"
     CDS             3290..3667
                     /locus_tag="VN377_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HWR26952.1"
                     /translation="MVKKDSGIAFNLISRKKLENYSSSDKLKYILNEVKEGRILVLEH
                     GLTPTEQAQLIEHTMKEINHDTFIGIEIGGYENEKVGFFQRVFGVAKKPRMTVIGPAH
                     LLRMVHKDNDMIETTIIPGKGVC"
     gene            3667..>4053
                     /locus_tag="VN377_00795"
     CDS             3667..>4053
                     /locus_tag="VN377_00795"
                     /inference="COORDINATES: protein motif:HMM:NF021372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon containing protein"
                     /protein_id="HWR26953.1"
                     /translation="MPHQCLKCGHVFEEGSSQLLKGCPGCGGNRFFFTKQPLSEVERD
                     VMSKEIGQDLTTRIMELAVEKHKETLKGTEAWITLKPKDLRRMIEDQKEQKQPEKEIT
                     LAEPISDDERRAIIDRIKAEADHSDRP"
BASE COUNT         1331 a          792 c          891 g         1039 t
ORIGIN      
        1 gctctgcatg gaagaacaaa agaaacacca tcacaaaata caagaggagc tttcgaaaat
       61 aaaaaaagaa tgcgaagtaa agaagatttc gtacgaaaaa ttgcttacta cgacaagaca
      121 acttgaagag aaaaaaggta aattcgaaag gaaacaacgc ttggaaaacg acatacaacg
      181 aatagaaaaa acattacgcg aacgaaaaga gacccttgaa aaactgaacg agacacttct
      241 acggttcaag actcttgata aagaactcca agattttgag aaacaacagg agaaaaacac
      301 gagtgttatt caaactagta ttaagaaaaa agagcagaac ctgaccctct ccactcgact
      361 cagagcggat atcaaagaat taacggaaaa aaaacacgca atagaaaaaa taggaccgca
      421 ggcaaaatgc ccaacctgcg accgagtact aggagaccaa caaaaaaaac ttatcgttgc
      481 gtttacgaaa gatattgaga aaaaaaatca tgagtcacga cgattcattg atgaacaaaa
      541 agaattacaa ctagagttcg acagatcatc tcgtgaaaaa caagcgctgc agaaaaaaaa
      601 tatgttcctg cgtagtcgag ttgttgaacg agaaaaatcc ctaaacacca ttcggcatac
      661 aacccaggag atggaacgag aaaatcaaga acttgaacat aaaaagaaag atctgaaaac
      721 tctcggtgtt gccgattttg atgaaaagca gtacagactg attcggaccc gtgtcaatga
      781 agcatatact ctctatcagg gatctttgac taaactttat gaaacaaatg aaaaacaaca
      841 aaaaatagct gtagaactaa aggaacaaga aggagaaaaa aaacttatcg accagcagat
      901 taagacctat gaaaaaaaca ttgaggacca acaaagattg gaaaaaaaac ttgaaaatga
      961 gcgaacggaa gcccaacacc agaacatgct ttccgaggtc atggaatcgt tccggacgta
     1021 tctcatcgct cagatccgcc ccatgctttc cgctcatgca tcggaacttt ttgatcagct
     1081 tacagatggg aaatactctc aaatagaact ggatgaagat tatgatctaa tggtgtacga
     1141 ccacggagca ccatatagca ttgaacggtt ctcggggggc gaagaagatt tggcaaacct
     1201 ctgcattcga ctagcgattt cagagatcat aaccgaaaga gcagggagtg tttttcaatt
     1261 cgttatttta gatgagattt ttggctcgca ggatcagata cggaagcaaa acatcattag
     1321 aacattgaat ggtttttctt ctaaattcaa acagattttc cttattactc acgtagaaga
     1381 catcaaacat ttcacggagc ataccatgat gatctcggaa gacgaaacag ggataagttc
     1441 gataaaaata gaataaaaaa aagaaaaaaa agttgggttg tttattttct gaaccgcagt
     1501 cttcgctttg gtttctcgat aggtgttggc tgatcttgga gggaaggagg aatttcgatg
     1561 tctgtttcag ttgtttctga agtaggagct gtcgtgtgct gttgttttag gtgttcgacg
     1621 gaggaaaaca gttttgtgaa gagttcctcg ttggtatcaa accagccttg ccaaccccgg
     1681 agttcctctt tcagctgttt attctccgat tcggtttcct catacaatcg gaagagtttt
     1741 gtgagtcgtt ctttttcctt ctcaaattcc attgatactt cttcaaattt tggtttgaaa
     1801 tccgcaaatt gttcaaagcc gcgtagttcc tcaacttgtc gttctaatcc tctgatgacc
     1861 tcatcctttg ttgaaatctc accgcgcatt cgatcaacat ttgtttccat ttgtgtgcgt
     1921 atggtgtcga atcgcatggc ctgttgtttc aaatcatcca tttgctggac tgcctgggct
     1981 cgttcatcta acttctgcgt cagagtctgg attcgtacgg tcgcatctcg ttcaaattgc
     2041 atgtgcatct gttcagtatc ccgcaacctc tcgtcaagct cggcgacacg tttctcttgt
     2101 gtagctaagg attcctcttg attcttatat gcgtcccaga gttttatcaa tcgttctttt
     2161 tctttctcaa gctccatact atagcgtcga gctccttccg ctgcctcatc ccgacgtcgt
     2221 tctgcttcag aaaggcgatc ctcaaacgat cgcatcatag aatccatttt gttcaatcct
     2281 tcgaaactga gcatgctttg gatttttgcg agatcctcca tacctttaga aaatgaactt
     2341 ttgacagaac ccagttcagt tgatgcttct tgcaattgtt ccataaatga atcttttact
     2401 cctttcacgc ctataccacc tactttttct tcagtcataa atcatgccgt cccatataac
     2461 atataattaa tactccatca agcgaataat gttgatgcta tttaagggtt attattttat
     2521 tgtgtttttc gcagaagaat cgtgagccgc taaactaata aagtattatt tgtatactat
     2581 ttaccaaatt tagaaaaata aattggagac agaaagggat gggattactc gagagattca
     2641 gagaaagtag attttcaaag atgatgttta aaatttttgg gaagaaccat gcgaagatcg
     2701 gaatctatgg tccaccaaac gtcggtaaaa ccacactggc aaatcgaatt ttacgggact
     2761 ggaccggtga tatcatgggg agtgtttctg aggttcctca tgagacgcga cgagcaaaat
     2821 ggaaaaacaa catcaggatc gataatgggg gatcgtcgtt gacgatggat cttgtcgata
     2881 cgccagggat tgcaacgaaa attgatttta aggaattcat gaacatgtac gggatgacaa
     2941 aggatgattc acgggtgcga gcaaaagaag cgactgaggg agtcatcgag gcgatccgat
     3001 ggcttgatga tattgatggt gtcgttttgg tcatggacgc gacacaggat cctttcacac
     3061 aggtcaatgt caccatcatt ggaaaccttg aggcacgaaa tctgcctatt ctcatcgcag
     3121 caaataagat tgatgcggaa aacgcaagtc cagcaacatt aaaatccgcg tttcctcagc
     3181 atccagtgat tcctattagc gctctgactg gatataacac ggagaatctc tatacgatga
     3241 tgataaaaga atttggaaaa aaacgacata aacagaggag gtaacaacca tggtgaaaaa
     3301 agatagcggg attgcattta atctgatttc cagaaagaag ctggagaatt attcttcctc
     3361 agataaactg aagtatattc ttaatgaagt aaaagaagga agaatccttg tgctagaaca
     3421 tggtctgact ccgactgaac aggcacagtt gattgaacat accatgaagg agatcaacca
     3481 tgataccttc attggtattg agatcggtgg atacgaaaat gaaaaagttg ggttcttcca
     3541 gcgggtgttt ggtgttgcaa aaaaacctcg gatgacggtg attgggccag cgcatctcct
     3601 cagaatggtt cataaagata atgatatgat cgagacgacg atcattcctg gaaagggagt
     3661 ctgttaatgc cacatcagtg tctgaaatgt ggtcatgtgt tcgaagaagg atcctcgcag
     3721 ttgttgaaag gatgccctgg gtgtgggggg aatcggtttt tcttcacgaa acagccgtta
     3781 agcgaagtcg aacgggacgt gatgagtaag gagatcggcc aagatcttac cacgagaatc
     3841 atggagctag cggttgaaaa gcataaggaa acgctcaaag gcaccgaagc gtggataacg
     3901 ctcaaaccaa aagaccttcg caggatgatt gaggatcaaa aagagcagaa gcaaccggaa
     3961 aaggaaatca ccctggctga gccaatcagc gatgacgagc gcagggcaat catcgatagg
     4021 atcaaagcag aggcggatca ttctgatagg ccg
//
LOCUS       DATMXJ010000025         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10699, whole genome shotgun sequence.
ACCESSION   DATMXJ010000025 DATMXJ010000000
VERSION     DATMXJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10699"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..551
                     /locus_tag="VN377_00800"
     CDS             369..551
                     /locus_tag="VN377_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26954.1"
                     /translation="MNEDDKKAFLNDFKKGDVDKKLDLWYYAVDQEALWEEILAEMSM
                     IAQAANPHKGKVPDEE"
     gene            563..766
                     /locus_tag="VN377_00805"
     CDS             563..766
                     /locus_tag="VN377_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26955.1"
                     /translation="MNEEDKKDLIEEFKTGDGARRLDMWDYALAQQVLWENIIAELQK
                     IAHEQGVDKELDKRMEDDMKGVE"
     gene            complement(776..1600)
                     /locus_tag="VN377_00810"
     CDS             complement(776..1600)
                     /locus_tag="VN377_00810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR26956.1"
                     /translation="MKGKIVLITGATSGIGKETAQGLALLGATVVLTTRDVERGEQTR
                     QELIQSTKNTNIDVLFCDLASFASIRDCCNEFLKKYPTLHVLINNAGTMDSHRRESKD
                     GIENMFAVNYLAPFLMTNLLLDVLKKSAPARIINLSSGLHSGTIQFNDLEFEHGFSGM
                     RVYGHTKLAIILFTRLLAEKLKGTGVTVNCVNPGMNATNLGRNAGWFSAKFFKLLGKK
                     PSIGAQTPIYLASAPEIETISGEYFEKKSVKKTNKGTYDMDAARKLWEVSKRYVGL"
     gene            1729..3012
                     /locus_tag="VN377_00815"
     CDS             1729..3012
                     /locus_tag="VN377_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWR26957.1"
                     /translation="MDSSWKQKRKLRLSDLIELDLQGLSPIQTIMKMAEPGAIRKMGL
                     NPEEVISFGGGWCNHTAPEALREAYQLIINDATLFHQSGRYSPIIGDSSCREQICRME
                     NAIYGLKSLESKNILLGHSSTQLFHDILRVVCNPGEPVGFLDPTYANYLNAVKCALPK
                     SPIWFIPALDPLTWTYLANPDESLEILKQYCAKGLRIFIIPVPDNPTSQIPSEEFLKS
                     ALEIVTDAGGYLLLDHAYKALWFGDMPRCYTWSPVDHCHLITLHSNSKWLSSLGRRFG
                     WAEAHETVINGLEKINESILLSPDSLHSMTTARFLEQTLSDGSLKQYVHDTRRLYEET
                     ASVMMSSLDSHLGWKRLTPDGGLYTCCPTPQQKEPRGFVERLLKATGVLLIPGTGFGP
                     SMRYGLRLSYGPLCYQHEQIAEGIERISQYLAKTG"
     gene            3086..4420
                     /locus_tag="VN377_00820"
     CDS             3086..4420
                     /locus_tag="VN377_00820"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HWR26958.1"
                     /translation="MKNLWVVFICICCVLPIAQATIVTQQQKKVSPTEASFIDGGWVE
                     ERDNITILHVSGSHYEMGFQHGYLLQEKVQQNIRAFLHYAEKYLPLSELLAYWNISKS
                     YVPPEYIEEMHGIADGANISFNDIVASIMAVEYSDHGCYGIAAWGIATLDGQLYHARS
                     FDLPSNIQDPVSGKYAHENTVLIVRNPDNGSASLCPAIAGSFHTGGGMNSHGVSLGIQ
                     ICWSKDQTFEGNPYHFRVQEVLDTATTAEEALQILNTNRTHGFNFIVSQADPAIGFVL
                     EQTANHTYIGTHNDSVESKQPFWAIEHVVRRTNVFIEPTIAETQRKPYNPSGLLAFLK
                     LLFFQKNNPFFAVFQLYKSVSKQINVTWGNLTLNTTMDVLRNGYQAEDFPLLRLIERL
                     GKGTGMAEAWNQWVACPATGDMVFSFANHDQMAFKTETHYVNFYDLLSESPP"
     gene            4497..>5393
                     /locus_tag="VN377_00825"
     CDS             4497..>5393
                     /locus_tag="VN377_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWR26959.1"
                     /translation="MQAPRIDLFEWLLQNAPHATHNLAFSNIHGLTIQEYKTFSDLPF
                     PDSFELGSNAQYGADALKKTLCTMYNCTSNNIVTTTGATEANFLVFSSLLGKGDEFIV
                     EQPGYQPMWSTPEMLGARRINLPRAFEHGFNIQTETFESLISEKTKLVILTNLHNPSG
                     VLTNLTTLKEIARIAVDHSIYVLIDEIFLDGSFAPVSSSFGIPNVIVTGSATKIYGLG
                     GLHSGWIIAPDEVAARCQRVKAHTTGTASYTSEIMTACILEAARFGLIKRFQQRAKTN
                     FDYLKRWMNNHTEFFEWVEPAGG"
BASE COUNT         1490 a         1125 c         1203 g         1575 t
ORIGIN      
        1 atctaacagt tgcatcaaat gaaaggtata acattcatta cagaccactt tgccatcagg
       61 taatttccga tacgaggtcc agagtttttt actgcatcgt tcgcagttga acattttgtc
      121 ccatcccata cgaatcccat accaaatatc ctatctttat ttaaatatct tttcaatttt
      181 acactttctg cgtatttttt agttataaat gtacataaat tggtttttat aaagagagat
      241 agtatatggt gctcttcggt gtcctgtttc caagtgcaaa gggtgaagac tccctggtga
      301 tgagtgaatc aaacaataaa caaatatata ggtcattact atttccgtac cgcttgtagg
      361 gagttcccgt gaacgaagat gataaaaaag catttctgaa cgacttcaaa aaaggagatg
      421 tcgataaaaa attagatctg tggtactacg ctgtcgacca agaagcattg tgggaggaaa
      481 tccttgcaga gatgtcgatg atcgctcagg cagcaaatcc tcacaaagga aaagtcccag
      541 acgaggaata agggtttttg ctatgaatga agaagataaa aaagatctca ttgaagaatt
      601 caagacgggc gatggtgcaa gacgcttaga tatgtgggat tatgcacttg ctcagcaagt
      661 actgtgggaa aatattattg ctgaattaca gaaaatcgcc catgaacaag gggtcgataa
      721 agagcttgac aaacggatgg aagacgatat gaagggcgtg gagtaagaat cgaggttata
      781 acccgacata tcttttgctg acttcccata attttctggc tgcatccatg tcataggttc
      841 ctttatttgt ctttttgaca gatttttttt cgaaatattc tccactgatc gtttcaatct
      901 ctggtgcact ggcaagatag atcggggtct gtgcaccgat ggatggtttt tttccaagga
      961 gtttaaaaaa tttcgcggag aaccagcctg cgtttcttcc aaggtttgtc gcattcatcc
     1021 ctggattgac acaatttact gtcacaccag ttccttttag tttctctgcc aaaagtctcg
     1081 tgaaaagaat gattgctagt ttcgtatgac cgtataccct catgccggaa aaaccatgtt
     1141 cgaattcaag atcattgaat tggattgttc cagaatgtag ccctgacgaa agattaatga
     1201 ttctagctgg tgcgcttttt ttcaggacat cgaggaggag gttggtcatg agaaatggag
     1261 caagataatt gactgcaaac atgttttcaa tcccgtcttt tgattctcgt cgatgggaat
     1321 ccatagttcc tgcattattg attaagacat gtaacgttgg atattttttg agaaattcat
     1381 tgcaacaatc tcgaattgag gcaaacgatg ccagatcaca aaacagtacg tcgatgtttg
     1441 tatttttggt cgattggatt aattcctgtc ttgtttgttc tcctcgttct acatctcgtg
     1501 tggtgagtac aaccgttgct ccaagaagag ctaagccttg tgcggtctct tttcctatgc
     1561 ctgatgtcgc acctgtgatg agaacgattt ttcctttcat aatcattttc cattatcttt
     1621 tttgttgagg aagacaacaa gaatatatta atctaatgat ttgagggaaa tcctgatggt
     1681 ttttctaaaa tggaaacatt aaagaaagat ctatcttttt tgcaatgtat ggactcttct
     1741 tggaaacaaa aaagaaagct ccgtctctct gatcttattg aactggatct ccaaggatta
     1801 tccccgattc agacgattat gaaaatggcg gaacccggag caattcgcaa gatggggtta
     1861 aatcctgaag aggttatatc gtttggtgga ggctggtgta accataccgc acctgaagca
     1921 ttaagggaag catatcaatt gataataaac gatgccacgt tatttcacca aagcgggcgg
     1981 tatagtccca ttattggcga ttcgtcctgc cgagagcaga tctgtcgaat ggagaacgcc
     2041 atctatggtc tgaagtccct ggaatcaaag aacatcctgc ttggtcattc atcaacgcaa
     2101 ctgtttcatg atatcctgcg tgtggtatgt aaccctggtg aacccgtggg atttcttgac
     2161 ccgacgtacg cgaattacct taatgccgtg aaatgtgctc ttcccaaatc cccgatatgg
     2221 tttatccccg cgctagatcc actgacatgg acgtatcttg caaaccctga tgagtcgtta
     2281 gagatcctca aacagtactg cgctaaagga ttacggattt tcatcatccc tgtccccgat
     2341 aaccctacga gtcagattcc ttccgaagaa ttcttaaaaa gcgccttgga gattgtcaca
     2401 gatgcaggcg gctacctcct cttggatcat gcatacaagg ctttgtggtt tggggatatg
     2461 ccacgatgct acacatggtc ccccgtagat cattgtcatt tgatcacgct gcattccaac
     2521 tcgaaatggt tatccagtct cggacggcgc tttggttggg cggaggcgca tgaaacagtg
     2581 atcaatggat tggaaaaaat caacgagtcc atactcttat ctcctgatag tttgcatagt
     2641 atgactacgg cacgtttctt agagcaaacc ctgtctgatg gttctttgaa gcagtatgtt
     2701 catgacactc ggagactcta tgaagaaaca gcgtcggtca tgatgtcatc gttggattcc
     2761 catctgggtt ggaaacggct cacccccgat ggtggactct atacctgctg tccaacgcca
     2821 caacaaaagg agccaagagg atttgtcgaa cgattattga aggcgacggg tgtcctgctt
     2881 atccctggaa ccggttttgg tcctagcatg agatatggtc tacgtctctc ctacggtccg
     2941 ctctgctatc agcatgaaca gatcgccgag ggaattgaac ggatcagtca atatctagca
     3001 aaaacaggat gacccctctc agggatgatt tcaaccagta tttttataaa gaggtgccct
     3061 tattgtggat atggaggatt cattcatgaa aaatctctgg gttgtattca tctgcatctg
     3121 ttgtgtattg ccgatcgcac aagcaacaat cgttactcaa caacaaaaga aggtctctcc
     3181 aactgaagct tcattcattg atggcggatg ggtcgaagaa cgagacaata tcacgattct
     3241 gcatgtgagt ggatcacatt atgagatggg attccaacat ggatacttgt tacaagaaaa
     3301 agtccagcag aatatccgtg catttttaca ttacgctgag aagtatcttc cattaagtga
     3361 gttgcttgca tattggaata tctccaagtc atatgtcccc cctgagtata tcgaagaaat
     3421 gcatggaatc gcagatgggg caaatatttc ctttaatgat attgtcgcat ccattatggc
     3481 ggtcgaatat tctgatcatg gatgttatgg gattgcagcc tggggaattg caaccttaga
     3541 tggacaatta taccacgccc ggagttttga tttaccctct aacattcaag accctgtgag
     3601 tgggaagtac gcccatgaga ataccgtcct aatcgtcaga aatcctgaca atggttctgc
     3661 ttcgctatgc cctgcgatcg ctggctcatt tcacaccggt ggtggtatga attcacatgg
     3721 tgtttccctt ggtatacaga tctgctggag caaagaccaa acctttgagg ggaaccccta
     3781 tcatttccgt gtacaagaag tattagatac cgcgacaacc gcagaggagg ctcttcagat
     3841 actcaatacg aatcggactc atgggttcaa ctttattgtc tcgcaggcgg atcctgcgat
     3901 aggatttgtt cttgagcaga ccgcgaacca tacctatatt gggacccata acgactcggt
     3961 tgaaagcaaa cagcctttct gggctatcga gcatgtggtg cgtcgaacga atgtgttcat
     4021 tgaaccaacg attgcagaaa cccaacggaa accctacaat ccatcagggt tgctcgcatt
     4081 tctcaagctg ttgttctttc aaaaaaacaa tccttttttt gcagtattcc aattgtataa
     4141 atcagtgagt aaacaaatta atgtcacttg ggggaattta acactgaaca ccaccatgga
     4201 cgtgctgcga aatgggtatc aagcagagga ttttccgctg ttacgtttaa ttgaacgact
     4261 cggaaaagga actgggatgg ccgaggcatg gaatcaatgg gttgcctgtc cagcgactgg
     4321 tgatatggtc tttagttttg caaatcatga ccagatggcg ttcaaaacag aaactcatta
     4381 tgtcaatttc tatgatctgc ttagcgaatc tccaccataa acacatattt ttttttgttt
     4441 cctttttttc ttttttctcc atactgtaat atatgtcctg tttgatgttt catcctatgc
     4501 aggctcctcg aattgatctg tttgaatggt tgctgcagaa cgctccacac gccacccata
     4561 atctggcttt tagtaatatt catggtctca cgattcaaga atataaaaca ttttccgatt
     4621 tgccatttcc tgatagtttt gagttgggtt caaatgctca atatggtgcc gatgcgttga
     4681 agaaaaccct ctgtacgatg tacaactgca ctagtaacaa tatcgttacg acgacagggg
     4741 ctactgaggc gaacttcctt gtgttttcat ctcttcttgg gaaaggagat gaatttattg
     4801 tcgagcaacc aggatatcag ccgatgtggt cgacccctga gatgctcggt gctcggcgaa
     4861 ttaatttgcc aagagcattt gaacatgggt ttaatattca gaccgaaaca tttgaaagcc
     4921 tcatttcaga aaaaacaaag cttgtgatcc tcacgaacct gcataatccc agcggtgtgc
     4981 tgacgaacct gacaacgctg aaggaaattg cacggatcgc ggtggatcat agtatatacg
     5041 ttctgatcga tgaaatcttt cttgacggat cttttgcccc ggtatcatca tcctttggga
     5101 ttccaaatgt gattgtgacc ggaagtgcga caaagattta tggtttaggc ggactccatt
     5161 caggatggat tatcgcccct gatgaggtag cagctcgctg tcagagagtt aaggcacaca
     5221 ccaccggtac tgcgtcgtat acgtcagaga taatgaccgc atgcatcctt gaggctgctc
     5281 ggtttgggct gatcaagcgt tttcagcaaa gagcgaaaac caattttgac tatcttaaga
     5341 gatggatgaa caatcatacg gagttctttg agtgggttga acctgctggt gga
//
LOCUS       DATMXJ010000026         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_21230, whole genome shotgun sequence.
ACCESSION   DATMXJ010000026 DATMXJ010000000
VERSION     DATMXJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21230"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1621
                     /locus_tag="VN377_00830"
     CDS             341..1621
                     /locus_tag="VN377_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR26960.1"
                     /translation="MNILLIEPHEGAKQTFHYRFSLFHSLSLEQIAALTPPEHNVTIL
                     NEKIQTIDYTRPYDLVGLSLMTCTANRGYTIADTFRVRGVPVVLGGWHPSAMPEEAKT
                     HADTIVIGEAELVWGLLLKDLQQGALKPYYQGDRLPTGDEIPAPQRISLRAYGPASIQ
                     ATRGCPTHCWFCCQHVVEGVHFRPRRINAVVDEITTLPNKLLHFIDSSLTINPTYSKD
                     LFRAMKPLGKQFECYGNINRLAKDDDLLRIAADAGCVRWFVGIESLSQETIDSMKKGT
                     NKVKDYPTAIKKVRDHGMMVTGLFMFGFDTDTPDVFDKTYQTACQWDLDCAQFNILTP
                     YPGTNVYEEYERQGRILTKDWSKYDVGHIVYQPKQMSQVDILQGIRRTASRYLSIPNA
                     MRRCINTHQMPASIWYNKVMRNISARGNIKIFKE"
     gene            complement(1611..2972)
                     /locus_tag="VN377_00835"
     CDS             complement(1611..2972)
                     /locus_tag="VN377_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWR26961.1"
                     /translation="MDSLPRKSLVFGIILLLLLNGISLITLKSSSTTAAERILIESEP
                     TNMDIDILGLINEVNKTILDSYVKDLLNFGSRPAGSKACQQAGAYIASKFSGFELDVS
                     VDNWTFFPVRSQNIVATLYGSDTSSDAEFLLSAHYDTRRASIGANDDASGIATILAIA
                     QICSQHYFNYTIRFVALSGEECGLCTDMPGSRDYARKAYDRGDNIVACFDLDVIGFCE
                     TEFGSRAVRVYTPIQASWIPLTMQQVCDDYPILNMTIEQMSNHPADQQAFISWGFDAV
                     LICQADQIRNVDKNDVYENINLSYITKVCRLMIAVVGVLAQTPITLQVRFTDPLRGYM
                     YAFGGKMKAPGLSRPFLREGATYLIGRTMARVNITSTEPIKQVAYGIDDLFIMRFNDT
                     QDTEWKIQGYDTPLIGRHTIMVYVITESGQIARDQMDLYCFTLSHMYFPWLAPFFYYF
                     FTP"
BASE COUNT         1177 a          884 c          765 g         1043 t
ORIGIN      
        1 attttaaagg ctattagttc attatcaccc attatttttt tctccctcaa acgacaattt
       61 taaacaatta tttctttaaa aatgtatcct atgagactca tgttttgtgc tgatggctaa
      121 acaaacttgt tgatttggtc cagggagttc aaatccattc ccctgcataa cttttccttc
      181 tcattggtct cagaagttac actctgctcg aaaaaatcca tttaacaaaa ctttttgaac
      241 cagacctctt atatataatc cataaggtac aaactgaaca ttcaaactgt cctacataaa
      301 aaaaatattt aaccccatcc taactatggt ggagcagagt atgaatattc tactcatcga
      361 gcctcatgaa ggagcaaaac aaacctttca ttatcgattc agcctgtttc attccctcag
      421 cttagagcaa atcgctgccc ttaccccacc tgaacacaac gtcaccatcc ttaacgaaaa
      481 aatccagacc atcgactaca cacgccccta cgacctcgtc ggtcttagcc ttatgacctg
      541 cacagccaac cggggataca ccatcgcaga caccttccgt gtaagaggag tacctgtcgt
      601 cctcggtggg tggcacccct ccgctatgcc tgaagaggca aaaacccatg cagataccat
      661 tgtcattggt gaagcagaac tagtctgggg attactactc aaggacctcc agcaaggtgc
      721 cctgaaaccc tactatcaag gagatcgcct acccactggt gatgagatac cagcaccaca
      781 acgtatcagc ctgcgagcct atggccccgc ctccattcaa gccacccgcg gatgcccaac
      841 ccactgctgg ttctgctgcc aacacgtcgt cgaaggtgtc catttccgtc ccagaaggat
      901 taatgcagtc gtcgacgaga taaccaccct ccccaacaag ctgctccatt ttatcgactc
      961 ctcccttaca attaatccga cctactcgaa ggaccttttc agagcaatga aaccacttgg
     1021 gaaacagttc gaatgttatg ggaacattaa tcgacttgcc aaagacgacg acctccttcg
     1081 aatcgcggcc gatgcaggat gcgtccgctg gttcgtcggc atcgaatccc tctcacaaga
     1141 aacaatcgac agtatgaaaa aaggaacaaa caaggtcaag gactacccaa ctgccataaa
     1201 aaaagtcagg gatcacggga tgatggtcac tggcttattc atgtttggct ttgacactga
     1261 cacgccggat gtcttcgaca aaacctacca gacagcctgt caatgggacc ttgattgcgc
     1321 gcaattcaac atcctaacgc cgtaccctgg aacgaatgtc tatgaggaat acgaacgtca
     1381 gggacgtatc ctcaccaaag actggtcaaa atatgacgtg ggacacatcg tctaccaacc
     1441 gaagcaaatg tcccaagttg acatcttaca aggtatcaga cgaacggcga gtcggtatct
     1501 ttcgattcca aacgcaatgc gtcgttgcat caatactcac caaatgcctg ccagcatctg
     1561 gtataataaa gtgatgcgta acatctctgc ccggggaaac atcaagattt tcaaggagta
     1621 aaaaagtaat agaaaaaggg ggctaaccac gggaaataca tgtgggataa tgtgaaacag
     1681 tacaggtcca tttggtctct ggctatttgt cctgattcgg tgatcacata gaccatgatc
     1741 gtgtgacgtc caatcagagg cgtatcgtag ccttgaatct tccattcggt gtcttgcgta
     1801 tcgttaaaac gcatgatgaa cagatcatcg atcccatacg ctacttgctt gataggttct
     1861 gttgaggtaa tattgactcg tgccatggtg cggccaatga gataggttgc tccttctctg
     1921 aggaacggtc gggatagacc aggagctttc atttttcccc cgaatgcata catatatcct
     1981 ctgagtggat ctgtgaagcg cacttggagc gtaatcggcg tctgggctag tactccaacg
     2041 acagcaatca tgagtcgaca cactttcgtg atataactaa gattaatatt ctcataaaca
     2101 tcgtttttat cgacattgcg tatctgatct gcttgacaga tgaggacggc gtcaaatccc
     2161 caggagatga aggcttgctg atcggcagga tggttcgaca tttgttcgat tgtcatgttt
     2221 aggattggat agtcatcgca gacctgttgc atcgtgagag gaatccacga cgcttgtatc
     2281 ggtgtataga cacggacagc tcgtgaacca aactcggttt cacagaaacc aatgacatct
     2341 aagtcaaagc atgcaacaat gttatctcca cggtcatacg cttttctcgc atagtcccta
     2401 ctacctggca tgtctgtaca caatccacat tcttctccag ataacgcgac gaaacgaatg
     2461 gtgtagttga agtaatgctg gctgcagatc tgagcgatgg caaggatagt agcgatgccg
     2521 gaagcatcat cattggcgcc tatggaagct cttcgcgtat cgtagtgtgc agacagaaga
     2581 aattccgcgt ccgatgatgt atcagaacca tacaaggtcg ccacaatatt ctgtgaacgg
     2641 actgggaaga atgtccagtt atcaacagat acgtctagtt caaagccgga aaacttacta
     2701 gcaatatatg ctcctgcttg ttgacaggct tttgaacctg ctggtcgaga gccaaagttg
     2761 aggagatctt ttacatacga atcaagaatg gttttgttga cctcattaat caaaccaagg
     2821 atgtcaatat ccatgtttgt tggttcggac tcaataagaa tacgctctgc agcggttgta
     2881 ctcgatgact tcaatgtaat tagggaaatt ccattgagta ataaaagtaa gattatacca
     2941 aaaacgagac tttttcgtgg taaagaatcc atagtattcc tctccaatat gagaatatat
     3001 ctttttttaa atataaaaac attgggtttt cggaaattta aaaatccaac agggtcattg
     3061 tctcgttttc cagtcctcat gatccctttc tttacatcga gcagtcgagc gtaacaatcg
     3121 ttttactatt gttacattac catatgtagg gaaacaacct gtattttgtc gtttttgagt
     3181 attcatcata cccctttaat tctctttgta atgtcttgtc ctcataataa attcgtataa
     3241 aaattaatga gacgtaaatt aacgctggaa gaagacaaaa ccatgaacca agtataaggg
     3301 gataacaaaa ccattggaaa atcatacata aataacccgg gtgccgtatg tatttatacg
     3361 gaccagttgc gcatactcgt tgtttactta aggtgtcatt tcgagttgtt agaataaaat
     3421 gagaattact aatttgagcc cataatgcga gaaaagacgt tagaacaata attaccatac
     3481 ttgggtatac cgcaatagat ggaatgcttg accattgaaa acgaacatca agtccagcaa
     3541 gaagtaacat cgctggtccg agtataaagt acgaaagtac gaaatatttg tcataatgct
     3601 tggtttccct caaattaaat tttccccttt cattcaataa ttgcggatta agaatcatca
     3661 atattgaaaa gtaaaaaatc tgatatgaaa accggtaaca aataaagatc catcctctca
     3721 cccaattaag agttcctgca gaaccaaata aaagtaaaaa ttcgacaagt acattagtaa
     3781 aattttttat aattcctcgt tttccaatga tatttagttt ttttgtatca ttgctcataa
     3841 aatccttcga attctagaca agatggaaa
//
LOCUS       DATMXJ010000027         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_63095, whole genome shotgun sequence.
ACCESSION   DATMXJ010000027 DATMXJ010000000
VERSION     DATMXJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63095"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VN377_00840"
     CDS             complement(<1..212)
                     /locus_tag="VN377_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase"
                     /protein_id="HWR26962.1"
                     /translation="MSVKIGFLVNPIAGMGGRVGLKGTDDVLDEAVKLGAYPIARQKA
                     EDMLHEFCRFPSNHQDVIWVTSDGDMG"
     gene            complement(391..612)
                     /locus_tag="VN377_00845"
     CDS             complement(391..612)
                     /locus_tag="VN377_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26963.1"
                     /translation="MKGDSQPYTIDVKTFLKHTSVGQSIVWPSNQTCIKVGKKTLTIP
                     NGKEGGETKHAPARQTDATQTRDTSVTGR"
     gene            complement(753..1889)
                     /locus_tag="VN377_00850"
     CDS             complement(753..1889)
                     /locus_tag="VN377_00850"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWR26964.1"
                     /translation="MDFDLTTNSVVTFTPLAGYTLPNLFRMLAQNRFHISPRYAARFV
                     YSIALSTIMSPFYLRERVKYDKSTEATPIMKDPIFIIGHWRCGTTLMHNILTRDQQFG
                     YFTTYQTLIPSIFISGEKLFKPIVVASLPKKRPMDDGDLDADLPQEDIYALGALSPYS
                     YYHGWCFPQNMEHYNNYIDFKNASSQTVEDWKNTYLFLLKKITMFHHGKQLILKNQDN
                     TGKIPQLLEMFPDAKFIYMYRNPYDLYMSMLKFMRKVIPLYCVQTPPPLKDVEDHMMT
                     LFTHMTKKYIQDKSLIPKENLVEVRYEDFINQPLRTVSTLYKKFNLNGYEDIESSLKA
                     YLTTQKTVHTDNYIFTDSIKKKVENHWGFALTEYEYTRPQEETR"
BASE COUNT          518 a          413 c          551 g          633 t
ORIGIN      
        1 cccatatctc cgtcgctcgt cacccagata acatcttggt gattcgatgg aaatcgacaa
       61 aactcgtgga gcatgtcttc tgctttctgt ctggcgatgg gatatgctcc tagtttgact
      121 gcttcgtcga ggacatcatc tgttcctttg agtccgactc ggccgcccat ccctgcaatg
      181 gggttgacaa gaaaaccaat tttcacgctc attttatgaa ggaaggattg ttctcaatag
      241 aaataggatt taaaaattga ttggttaaaa ccagtgactt tttcattttg agattttccc
      301 gcgatgtatt taaatagggt gaatgagaga ataggaaagt ggtgaggaag ggcgacgttc
      361 ggcggggtct tgtcttcccg atctgcgctc ttatcttccg gtaacgctgg tgtcgcgtgt
      421 ctgcgttgcg tcggtctggc gcgctggtgc gtgcttcgtt tcccctcctt ccttaccgtt
      481 tgggatggtg agtgtctttt ttccgacctt gatgcatgtc tgattggacg gccatacgat
      541 cgactgaccc accgaagtat gttttaggaa tgtttttaca tcaatggtgt agggttgtga
      601 atcacccttc attaaatatt cagaggtatc gttgtttttt tttaaaaata agaggaatcg
      661 gacctacaga gttcaggttt tcgctcctct gtcgaagatc gtgacaacca tccgttacga
      721 tagcgtatcg aaccgtcagt agcactcgtt cctcaacggg tttcttcctg ggggcgagta
      781 tattcatatt ctgtgagtgc aaagccccag tggttttcaa ctttcttttt tatcgaatcg
      841 gtgaagatgt agttgtccgt gtgaacggtt ttctgcgtgg taagataggc tttcagggaa
      901 gattcaatgt cttcgtatcc attgaggttg aatttcttat acagggtact gacggttctc
      961 aggggttgat tgatgaagtc ttcgtaccgc acctccacga ggttctcctt gggaataaga
     1021 gatttgtctt ggatgtattt ctttgtcatg tgcgtaaaca acgtcatcat atggtcttca
     1081 acatccttca gagggggtgg ggtttgcacg cagtacaggg ggataacctt gcgcatgaac
     1141 ttcagcatgg acatgtagag atcataaggg ttgcggtaca tgtagatgaa tttcgcgtca
     1201 ggaaacatct ccagcagctg cgggatcttc cccgtgttat cctgattctt caagataagc
     1261 tgtttcccat ggtggaacat ggtgatcttt ttcaaaagga aaaggtaggt attcttccag
     1321 tcttcgactg tctgagagga ggcgttctta aaatcgatgt agttgttgta atgctccata
     1381 ttctgaggga agcaccagcc gtggtaatag gaatagggag agagcgcacc aagggcgtag
     1441 atatcttctt gtgggaggtc tgcatcaaga tcaccgtcgt ccatcggtcg tttctttggg
     1501 aggcttgcga caacgattgg tttgaagagt ttttcaccgc tgatgaaaat agaaggaatc
     1561 agggtctggt aggttgtgaa atatccgaat tgttggtccc gtgtcaggat gttatgcata
     1621 agggtcgttc cgcaacgcca atgaccaatg atgaaaatcg gatctttcat gataggtgta
     1681 gcttcggtgg atttgtcata tttgacgcgt tctctgaggt agaacgggct catgattgtg
     1741 ctaagggcta tgctgtagac gaaccgcgca gcgtaccgag gactaatgtg gaagcggttc
     1801 tgcgccagca ttcgaaagag gttgggaaga gtatatcctg ccaacggggt aaaagtgact
     1861 actgaattgg ttgtgaggtc aaaatccata gtgagatata aaatcatctt gtctatataa
     1921 aatatgcggg attccgaatc gtaccaggaa attcacgaag gtttgattcc acataatgaa
     1981 atgattatag ttttaacata acaaacagta aaaaaaaatg gggtttttgt cgtacgattt
     2041 cctctaagag aaattagcgg acctatgggg atttgaaccc cagtatccgg ctccgaaggc
     2101 cggaaggata tccag
//
LOCUS       DATMXJ010000028         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_31816, whole genome shotgun sequence.
ACCESSION   DATMXJ010000028 DATMXJ010000000
VERSION     DATMXJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31816"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1098)
                     /gene="pyrF"
                     /locus_tag="VN377_00855"
     CDS             complement(286..1098)
                     /gene="pyrF"
                     /locus_tag="VN377_00855"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWR26965.1"
                     /translation="MDWREKLSSITSQNNSLLCVGLDSDTGKIPKFLMTSSKNPLFDF
                     NKEIIDATKDLVCAYKLNMAFYEVAGNKGILALEKTIRYIPHDILIILDGKRNDIGNT
                     AQKYAQSLFDILNADAATINPYLGKDGVNPFLEYKEKCCFILCRTSNPSAGDFQDLLV
                     SSTPMYQIVARKIREWDTNKNCGAVVGATYPGELKTVRSILGDDIPILIPGIGKQGGD
                     VEKTVRNGTNSHGEMVLINSSREIIFAGDNEDFAGQARKKAESVRNDINKYR"
     gene            1164..2258
                     /locus_tag="VN377_00860"
     CDS             1164..2258
                     /locus_tag="VN377_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HWR26966.1"
                     /translation="MENTIQHMEVIYGVCSWGLGHATRSLPVIRKLINEKHQVTVISN
                     GRSLEVLKKELGSDIAYFDIPDYPMLLSENTRQFLAKSMVYWPVFIKRIEDGLSQLQK
                     ILDKKNYDCIISDARYDMYSKKIPSFFISHQMRIMNPLQIQTFERAMERFNMFFFKRY
                     VGVMVPDYKENNLSGDLSHNLKRIDEDTIHYVGVLSDFTKRPLKKDIDYLISISGPEP
                     QRSILEEKLASQVSDLEGNVVMTLGKAEKYSIVKKKHLTTYSFVTKDLREELLNKAKL
                     VVSRSGYSTIMDVAVVGAKALFIPTPGQIEQEYLSQYHNTLGTFYSVSQDAIDLRSDV
                     KRAAEKTGITRECDVKKTVENILRVIMEKT"
     gene            2323..>3308
                     /locus_tag="VN377_00865"
     CDS             2323..>3308
                     /locus_tag="VN377_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWR26967.1"
                     /translation="MRMWGFLKSWYFWLILITGIAIVVRSFPAWMNAAWGGDFGIYYG
                     LTSRFVENQQIFNEYNGWGGMYNYFPVLYIFSALGHWITGVDLLWVMPKIAPIFGGLT
                     VCIFYFIVYDLTKRRDLALLSSLFLAVIPFHVYQTSHAAPLTMGHFFMMLSCYLFLKY
                     MDQKKYLIPLMISTVFLIMSHHLTTYFFLITIFFIVVIKSIRVDLRTMYRDVAYVLVA
                     SALTFSYWIFIATPVFSTFMNNGLSISSYLVIVLFYVSLFGCLFTNAAMKKKKPQFIY
                     NLKKMLSFNPAHSRKRALIYFTAGFVFIFCAEIVFLFVNFPVSGIRMKPLSIL"
BASE COUNT          887 a          753 c          698 g          970 t
ORIGIN      
        1 acccaacaca aaaataataa tatgtattgg gtgtggtgac acagtatcct ccacagtaag
       61 cagcagcggg agaatcctgc agcgcttctc gcgacagttt tatgatatcg tggttatcct
      121 ctgtttcatc tcttgacttt acgtctgctt gatcaaagag ggaccctagt cgatatcaat
      181 tgtcaccttg aacttgcttc tccaatcatg ttatcatgga gatcttcgta tcaaggataa
      241 caaaacatac caccatcgat taattaaggt ttcgctccct agttttcacc gatacttgtt
      301 gatatcattg cggaccgatt ccgctttttt tctcgcttgc cccgcgaaat cctcattgtc
      361 tccggcaaaa atgatttccc gcgaggagtt tataagaacc atctctccat gagaattcgt
      421 cccgtttctg acggtttttt caacatcgcc gccttgtttt ccgatcccgg ggatgagaat
      481 agggatatca tcgcccagga tactgcgaac ggttttcaac tcccccgggt aggtcgcacc
      541 gacgaccgca ccacagttct tattggtatc ccactcgcga attttccggg caacgatctg
      601 atacattgga gttgatgaaa caaggagatc ctgaaaatca cctgcggagg gattggaggt
      661 acgacacaag ataaaacaac atttctcttt atactccaag aaggggttca caccatcctt
      721 ccccagataa gggttaatcg tggcagcatc agcgttcaga atatcaaaga gggattgggc
      781 gtatttctga gcggtatttc ctatatcatt tcgcttccca tcaagaatga tgaggatgtc
      841 atgcgggatg tatcgaatgg ttttttccaa tgctaggatt cccttgttcc cagcaacctc
      901 ataaaacgcc atattcagtt tgtacgcgca caccaggtct ttggttgcat caataatttc
      961 tttgttgaaa tcgaacaacg ggtttttact actcgtcata aggaactttg gtattttccc
     1021 agtatcagaa tcaagcccaa cacagagcag gctattgttt tgagatgtga tactcgataa
     1081 tttttctctc cagtccatac aatccccaag tgaaatagta tttcttctat aaatactcgt
     1141 gttttcgtta gctatttaac gtcatggaaa atacaatcca gcatatggaa gtcatctacg
     1201 gagtatgttc atggggtctt ggccatgcga cacgatcctt accggtcatc aggaaactca
     1261 tcaacgaaaa acatcaggtg accgtgattt caaacggacg aagtcttgag gtactgaaaa
     1321 aggaacttgg aagtgacatc gcctattttg acatcccgga ttatccgatg ttgctgtctg
     1381 agaacaccag gcagttcctt gcaaaaagca tggtatattg gcctgtgttc atcaaaagaa
     1441 tcgaggatgg attgtcgcaa ctacagaaga ttcttgataa aaaaaattat gactgtatca
     1501 tctctgatgc tagatatgat atgtacagta agaagatccc gtcgtttttt atctctcacc
     1561 agatgcgcat catgaaccct ctgcagatac agacgtttga gcgtgccatg gaacgattca
     1621 acatgttttt tttcaaacgc tatgttggcg tcatggtccc agattacaaa gagaacaacc
     1681 tctctggtga tctttcacat aatctcaaaa ggatcgatga agacacaatc cattatgtcg
     1741 gagtgctgtc tgattttacg aaacgaccat tgaaaaagga tatcgattat ttgatttcga
     1801 tatctggccc agaacctcaa cgaagcatcc ttgaagaaaa actcgcttca caggtttcag
     1861 accttgaggg aaacgtcgtg atgaccttag ggaaggcaga gaaatattcc atagtaaaaa
     1921 agaaacatct gacgacgtat tcgtttgtga caaaggatct ccgtgaggag ttactaaaca
     1981 aggcaaaact ggtggtgtca cgttcaggat actccacgat tatggacgtg gctgtggtcg
     2041 gggcaaaagc attgttcatc cccacgccag gtcagataga acaagagtac ctctcacagt
     2101 atcataatac gcttggcacg ttttattccg tgtcgcagga tgctattgat ctgagatcag
     2161 atgtgaagag agcagcggaa aaaacaggga taaccaggga atgcgacgtc aaaaaaaccg
     2221 tggaaaatat ccttagagtt atcatggaga aaacataaaa acctgtacca taacagcttt
     2281 aaatcagatg aatattaccc tttgaggaag gaaattttcg ttatgagaat gtggggcttt
     2341 ctgaaatcat ggtatttctg gttgatcctg attaccggga ttgctatcgt tgtccgttca
     2401 tttccagcct ggatgaacgc tgcgtggggt ggcgactttg ggatctacta tggactgacc
     2461 tcgcggtttg tcgaaaacca gcagatcttc aacgaataca acggctgggg cgggatgtat
     2521 aactacttcc ccgtactgta tatcttctca gcgttgggcc attggatcac cggggtcgac
     2581 cttctctggg tgatgccgaa gatcgcaccg atcttcggtg gtttgactgt ttgtatcttc
     2641 tatttcattg tctatgatct gacaaaacgg cgagaccttg cgctgttatc atcactattc
     2701 ctcgcagtca tccccttcca cgtgtatcag accagccatg cagctccttt gaccatgggg
     2761 catttcttca tgatgctttc ttgttatctc ttcctgaaat acatggatca gaaaaaatac
     2821 cttatcccat tgatgatttc cacggtcttt ctcatcatgt cgcatcatct caccacctat
     2881 ttcttcctca tcaccatctt ctttatcgtg gtcatcaaaa gcatccgcgt cgatctccga
     2941 acgatgtacc gtgacgttgc ttacgtactt gtcgcatcag ctttgacctt tagctactgg
     3001 atctttatcg ccacaccagt cttcagcacg tttatgaaca acgggctgtc tatttcctct
     3061 tatcttgtga tcgtcctttt ctacgtcagc ttattcggtt gtcttttcac aaacgctgcg
     3121 atgaaaaaga agaaaccaca gtttatttac aacttgaaaa aaatgttatc gtttaaccct
     3181 gcgcattcaa ggaagcgcgc gttgatctat tttactgcag ggtttgtgtt tattttctgc
     3241 gcggagatcg tgtttttatt tgtgaacttc ccggtcagcg gtatacgcat gaaaccgctc
     3301 tccatcct
//
LOCUS       DATMXJ010000029         5636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_63198, whole genome shotgun sequence.
ACCESSION   DATMXJ010000029 DATMXJ010000000
VERSION     DATMXJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63198"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="VN377_00870"
     CDS             complement(<1..1886)
                     /locus_tag="VN377_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR26968.1"
                     /translation="MKRTKTHNSKIGRLVVGVAICVLMLTTSVTTALINPQDFGNQTP
                     VGRSQLTYTWSFLEPNYQSISADGSEYTIVNMPGLIGGGAEQGAPQLPMKMIQLLLPP
                     KTAVTSITVTGTPVVVSQAVDLIAQPVLPYQRSVPFGSDEPQVFVKNLDIYSANTPYP
                     SKVCTDHHIGYSHGYAILDVGLSPLQYTPSSGTLMFYPKMTVTITLENTGYVNELFRN
                     SPDDKTYVQTLVANPEIAQLYETSDLPTFEYPGGLCDPSQHYDYVIITTTQSGLDYWD
                     TTTVTPYNWESLMDEHSGDGLTSTLVTVQDIDACTDYFGINPFNDQQAHVREFCKDAY
                     EDWGTSYILVAGDSNFIPARLMSSGGESNVDADLYWSNLDSTFNGDSDSQWGEEGDSG
                     FDLYSELFVGRVTCDVPQDVSNWLTKSFYYADSAEWDYLDNAAFYGGDTTWTCQGDDF
                     MDYSAIKGTDEWLGPTPDTFPSWVGFQFGFETWNEVNPGNMFNLSVKYTEAPSPNPGW
                     SGAGALGLKNAINNDLVAFISGIAHANNQMSLDVYDTSWASDYHNTKPFFVHDYGCHC
                     GDFDDGDDGVLETMLFHSDTELSFGCVYNTGYGWGNFYCTNSSSAFQAKEFWRFFLDV
                     ENLS"
     gene            complement(2103..4136)
                     /locus_tag="VN377_00875"
     CDS             complement(2103..4136)
                     /locus_tag="VN377_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR26969.1"
                     /translation="MSGNVKWKHQTFLLRLILCVTAIVLSSVAGSIVPPVSEFRQTMI
                     PEGNDWWPMFHHDATHSGVSSTTAPDTADVLWSYQTGFIISSSPAVSHGRVYIGSWDK
                     SIYCFEMDSGNLLWNHSTSSEITSSPAVVDGRVYVGSQDTYLYCLDAIDGSLLWDFKT
                     GFIVETSPIVVDDKVFFGSNDGSLYCLNTEDGSLVWEYVTNSAFVSSPAVLDENLYVG
                     IVNGDFICLNSLTGNHLWTFTTTSGIYSTPTLDDGKIYFGANDHNVYCLDAENGSLIW
                     SYNAESEVHSSPSIAYGSLYVGTSDGRMLCLDKDTGDFLWSYVINGSVESSPGVADGN
                     VYFVTDPCCGYTSYLFCLNAYTGALLWNYNLNTQLHTKSSPALAAGKLFVGSGDGTVY
                     AFGDIQYLADANGPYYGFVNSSVEFIGSVYGGEPGFSWYWDFGDGTTSTLQNPTHTYP
                     SIGEYTVILTVTDSLGQIATDDTLVYIDVPNVPPEIPTIDGPTTGKPAESYEFFFTAS
                     DQNNDKVLYDINWGDNMTSGWIGPFGPGITISQNHTWSTRGSYEIKAKVKDWHGAESI
                     WSDPFEFSITAPELTIEIKGGFGVTVTIRNTGDAPATNVSWNISFDGTFVFPSFNYQI
                     LGTIQPGATKSENILMLGFGKTTMHAIASCDEGISVNKTVQGKLLLFFVFGIR"
     gene            4308..5297
                     /locus_tag="VN377_00880"
     CDS             4308..5297
                     /locus_tag="VN377_00880"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWR26970.1"
                     /translation="MKILITGITGFLGTHLSNVLLNTNKFELIGTYRDAVKATIFEKQ
                     GIEMRKADLLKPESLKDITKDMDVVVHLAGLMRFHDPFDLLYDNNVKATQLIAQDASV
                     HGVQHFIYSSSTEAIGPVTTIPGDETSPYRPTYDYGKTKQIAETWLKEKQTTGLPLTI
                     LRPTGIYGPGDMYVTLSTVRAVANKKLQFLPGKGDTYIHFTYVDDITQGIQRTIEKPK
                     QSIGETFILASDAYITYKEMFTIVAGLLDVPAPSRSIPLSLAKAYLSVVQWNNTRKGI
                     DDFVMHTSLIDTMRTNRAYSNAKAKKILGFSPRFSYQEGMKKTIDWYKEKNLL"
     gene            complement(5452..>5636)
                     /locus_tag="VN377_00885"
     CDS             complement(5452..>5636)
                     /locus_tag="VN377_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26971.1"
                     /translation="TTLVLPSKIGFYFGQENMVVQLQGAKGIFFRGEKSFFFSNTPER
                     IVAVCKATDIWVTYNI"
BASE COUNT         1620 a         1342 c         1217 g         1457 t
ORIGIN      
        1 ctggataggt tctccacatc aaggaagaac ctccagaatt ctttggcttg gaacgcgctg
       61 cttgaattcg tgcagtagaa attcccccag ccatacccgg tgttgtagac gcagccaaag
      121 gaaagttccg tatctgaatg gaagagcatt gtttcaagga cgccatcatc accatcatca
      181 aagtcaccac agtggcatcc gtaatcatgg acgaagaatg gtttcgtgtt gtggtagtca
      241 gatgcccaag aagtgtcgta gacatccaaa gacatttggt tatttgcgtg ggcgattcca
      301 ctgataaaag cgaccagatc gttgttaatc gcgttcttta atcctagtgc tccagctcca
      361 gaccagcctg gattgggcga tggtgcttcc gtatatttca cggagaggtt gaacatgttc
      421 ccagggttga cctcgttcca ggtctcaaaa ccaaactgga aaccaaccca gcttgggaat
      481 gtatccggcg tgggtccgag ccattcatcg gttcccttga tggcgctgta gtccatgaag
      541 tcatcgcctt ggcaggtcca agtcgtatca ccgccataga acgctgcatt gtctaagtaa
      601 tcccactcgg cgctatccgc gtagtagaaa ctctttgtca accagtttga aacatcctgg
      661 ggcacatcac aggtaacacg tcccacgaat aactcagaat aaagatcaaa accagagtca
      721 ccttcctcgc cccattgact gtcagaatcg ccattgaacg tactatcaag gttgctccag
      781 tacaagtcag cgtcaacgtt tgattcgcca ccggacgaca tcagtcgggc agggataaag
      841 ttcgagtcac cagcaacaag gatataactt gtcccccagt cctcataggc gtctttgcag
      901 aactctcgca catgagcctg ttggtcattg aatgggttta tgccaaaata atcagtgcac
      961 gcatcgatgt cttggaccgt gacaagcgtg gatgtcaagc catctcccga atgttcgtcc
     1021 atgagacttt cccagttata cggagttacc gtagtggtgt cccagtaatc taaaccactc
     1081 tgggtggtcg tgataatcac ataatcataa tgttgacttg ggtcacagag tccccctgga
     1141 tattcaaacg tcggcaaatc actggtttcg taaagctgcg cgatctctgg gtttgcgacc
     1201 agcgtctgaa cataggtttt atcatcaggg ctgttgcgga acaactcatt gacatatcca
     1261 gtgttctcaa gggtaatggt gaccgtcatc ttcgggtaaa acatcagtgt accactgctg
     1321 ggtgtatatt gcagcgggct taatccaacg tcaaggattg cgtacccatg ggaataccca
     1381 atatgatgat cagtgcatac ctttgatgga tacggcgtgt ttgcggaata gatatcaagg
     1441 tttttcacaa acacctgcgg ttcatcgctt ccaaacggaa cagatctctg gtacggaaga
     1501 accggctgag cgatcaagtc gactgcttgg gaaaccacaa ctggtgttcc cgtgaccgtg
     1561 atacttgtca ctgcagtttt tggtggtaat aagagctgga tcattttcat cggtaactgt
     1621 ggtgctcctt gctctgcgcc cccgccaatt aaacccggca tgttcacaat ggtatactca
     1681 gagccatcag cagagattga ttgataattt ggttctaaga aactccatgt gtacgtcaac
     1741 tgagagcggc ctactggtgt ctggttgccg aaatcttgtg gatttatgag tgctgttgtt
     1801 acggatgtag taagcattaa gacacagatt gctacaccaa caacaagtct gcctatctta
     1861 gaattatgag tttttgttct cttcatgtat ttttttcccc tatataagcc atggcgctcc
     1921 tgtcatttaa attttttggt tatataaata aatatgaggg gtcgaagtct tttttcttgg
     1981 agaaaaccac tctctatggt gcttttttcc ttcatatttt tatcctgtca tcatggtcat
     2041 cttatttttt gggataaaag ttggtcaaaa aaggtcagaa aaagaaaata aaataattag
     2101 aactatctta tcccgaaaac aaagaacaac aaaagtttcc cttgtactgt tttattgaca
     2161 gatatcccct catcacacga ggctatggca tgcattgttg ttttaccaaa cccgagcatc
     2221 aggatatttt ctgatttcgt cgcaccaggt tgaatggttc ccaggatttg atagttaaat
     2281 gagggaaaaa caaacgttcc atcaaacgaa atgttccagg agacatttgt cgcaggggca
     2341 tcccccgtgt ttctgattgt taccgtgaca ccaaacccgc cttttatttc gatcgtcagc
     2401 tctggtgcgg tgatagagaa ttcgaaggga tctgaccaga tgctctcagc accatgccag
     2461 tcttttacct tcgccttaat ctcataggat cctctggtgg accaggtatg gttttgactg
     2521 atcgttattc ctggcccaaa cggtcctatc catccactgg tcatattatc cccccaattg
     2581 atgtcgtaca ggaccttatc attgttctga tctgatgcgg tgaaaaaaaa ctcgtaggat
     2641 tctgctggtt tcccggttgt cggaccatct atcgttggta tctctggtgg gacgtttggg
     2701 acatcaatat agaccaaggt gtcatcggtt gcgatctgac caagactgtc ggtcacggtg
     2761 agtatgacgg tatactcccc gatggacgga taggtatgtg ttggattctg aagagtcgat
     2821 gtcgtaccat caccaaaatc ccagtaccac gaaaacccgg gttcaccgcc atagaccgaa
     2881 ccgatgaact ccactgaaga attcacaaaa ccatagtacg gaccgttggc gtcagcaaga
     2941 tactggatat cgccaaaggc gtagacggtt ccatccccgg agccaacaaa cagcttgcca
     3001 gcagccagag caggtgatga tttcgtatga agctgcgtat tcagattata attccagagt
     3061 agcgccccgg tatacgcatt caaacagaac aaataacttg tgtacccaca gcaggggtct
     3121 gtgacaaaat agacgttccc atcagcaaca ccgggtgaag attcgacgct gccgttgata
     3181 acgtaactcc acaggaaatc ccccgtgtcc ttatcaaggc agagcatcct gccatcactc
     3241 gttccaacat aaagggaacc ataggcgatc gatggggagg aatgcacctc actttccgca
     3301 ttataactcc agatgaggct tccgttctct gcgtcaagac aatagacatt atgatcattc
     3361 gcgccgaagt agatttttcc atcatccaga gttggggttg agtagatgcc cgacgtcgtc
     3421 gtaaatgtcc agagatgatt cccagtgagg ctgttcaggc agatgaaatc tccattcacg
     3481 atgccgacat agaggttctc atcaagaacc gctggagagg aaacaaacgc actgttggtc
     3541 acgtactccc aaacaaggct cccatcctcg gtattcaaac aatacagcga accatcattt
     3601 gatccaaaaa aaactttatc atcgacaact atcggtgagg tttcaacaat aaaacctgtt
     3661 ttaaaatccc agagcaatga accatcgatt gcatctagac aatacaggta ggtgtcctgt
     3721 gagccgacat acactctccc atctactacc gccggggatg atgtaatctc actgcttgtc
     3781 gaatggttcc ataacagatt tccactgtcc atctcaaagc agtagatgct cttatcccag
     3841 gaaccgatat agacgcgtcc atgggacacc gcaggtgacg aagagataat aaaaccagtc
     3901 tggtaactcc aaagcacgtc agcggtatct ggcgcggtcg tcgaagacac accgctgtgc
     3961 gtcgcatcat ggtggaacat cggccaccaa tcattccctt caggaatcat cgtttgcctg
     4021 aactcggaaa caggtggtac aatcgaacct gcgactgaag acaagactat tgccgtgacg
     4081 cacaagatga gacgaagcaa gaatgtctga tgtttccatt tcacgtttcc gctcatagta
     4141 gtaccttaag tgaaatattg tttattctat ttattacaac caataatccc ctatataatt
     4201 ttaacgattg ttaataaatg accttctcca ctactagcct agcagaatat caataggcat
     4261 aatatcataa aacgtattca caatgaggac cagaggagga cacatcaatg aagatattga
     4321 tcaccggaat aacaggattt cttggaacac atctttccaa tgttcttttg aacacaaata
     4381 aattcgaact tatcggaaca tacagagacg cagtaaaagc aacaattttt gaaaaacaag
     4441 gaatcgagat gagaaaagca gacctgctca aaccagagtc tctcaaagac atcacaaagg
     4501 atatggatgt tgtcgttcat ctcgcaggat tgatgcgttt tcatgacccc ttcgacctct
     4561 tatatgataa taacgtgaag gcgacccagc tcattgcaca ggatgcttcc gtccatggcg
     4621 tacaacattt catttacagt agttcgacag aggccatagg ccctgtgacc acgattcctg
     4681 gagatgaaac aagcccgtat cgtcctacct atgattacgg gaaaaccaag cagatcgcgg
     4741 aaacctggtt gaaagaaaaa cagacaacag gtcttccgct gaccatcctc agacccaccg
     4801 gaatctacgg acctggtgat atgtacgtca cactgtccac ggttcgagcg gtggcaaaca
     4861 aaaaattaca gttcctgcct ggaaaaggag acacctatat ccacttcacc tatgtcgacg
     4921 acatcacaca aggtatccaa aggacgattg aaaaaccaaa acaatccatt ggcgaaacct
     4981 tcatccttgc aagcgatgcg tacatcacgt acaaagaaat gttcaccata gtagctggtt
     5041 tgttagacgt ccctgcgccc tctcgctcca tccctctgag tcttgcaaaa gcataccttt
     5101 ccgttgtcca atggaacaat accagaaaag gcatcgatga ctttgtcatg catacgtcac
     5161 tcattgatac tatgaggacg aatcgggcat acagtaatgc aaaggcaaag aaaattcttg
     5221 gattttcacc acgattttct tatcaagaag ggatgaaaaa aacaatcgat tggtataagg
     5281 aaaaaaacct cctttgaatg aacttaagag ataaaatcta ttaaaaaaaa gaagggatac
     5341 attctatttc aaaggaaatt gattccgaaa aaatctcgga agagtactat gaggctagga
     5401 tcactccctc cacctgatag aaaaccgatt ctacctgatt tcccgcgttt ctcaaatatt
     5461 ataggtcacc cagatatctg ttgctttaca cacggcaact atcctttccg gtgtgttcga
     5521 aaagaaaaag gatttttccc cacggaaaaa aatacctttg gctccctgca actgtacgac
     5581 catgttctct tgaccgaaat aaaaaccgat tttcgaaggc aggacgagcg tagtaa
//
LOCUS       DATMXJ010000030         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_58023, whole genome shotgun sequence.
ACCESSION   DATMXJ010000030 DATMXJ010000000
VERSION     DATMXJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58023"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VN377_00890"
     CDS             complement(<1..671)
                     /locus_tag="VN377_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR26972.1"
                     /translation="MKIVHFSWEYPPVIHGGLGTFATEISKKQVELGHDVTVFTLNRQ
                     NTLPTSDQINGVDVYRPKTLDLSKPFYLCADHELRSWGPYFTFFADVLSYNIMSASKL
                     VNVLVRKNGRTFDIIDAHDWLGIMAGMVAKKELNIPLIFHVHSTEVGRSIGRGSHTIK
                     DIEYEGGQIADCVITVSNAMADELLKLGFPQEKIRPCWNGVDPAKYDPAQVSNEDRLA
                     LRRSYG"
     gene            complement(688..1878)
                     /locus_tag="VN377_00895"
     CDS             complement(688..1878)
                     /locus_tag="VN377_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HWR26973.1"
                     /translation="MPSICFYFEVHQPFRLNHFSVFNIGNTKDIHSTYFDKHLNQKIF
                     EKVANKCYLPTNNLLLELIRKYDGKFRISYSLTGTFVEYCENYLPELLDSFKELFKTG
                     AVDLIEETYFHSLSGLYDELDEFEDQIKMHRQMIRRIFNYKPKVFRNTEAIYDNRIAR
                     KISDLGYKGILTEGAEKILGWRSPNFIYKAANANLKVLLRNYKLSDDVGFRFSARNWP
                     GFPLTADKYANWMSNSYGDVINLFMDYETFGEHQWIETGIFEFLKHLPGEVLKHDSLD
                     FATVSEAVERYNAVGDVDSPWAISWADEDRNVSTWLGNNMQIACFNELKDIGRKLKRK
                     GDRDLLYIWRLLQTSDHLYYISTKGFEDGNVHAYFSPYDIPYDGFINYMNILQDLKEK
                     AENR"
     gene            complement(1880..2989)
                     /locus_tag="VN377_00900"
     CDS             complement(1880..2989)
                     /locus_tag="VN377_00900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWR26974.1"
                     /translation="MKIAMIGWEYPPFKVGGLGTHCYGLTHGLADKNVHIDFYMPKLR
                     QKVKSDNKNITIIEVGESEVYPYDRPDDKEIGGKFFDAVYRYNDLLVKRIKGKYDVIH
                     CHDWLTMKAAIAVKEKLGVPLVLTVHSTEYDRSGWIYPNQWFIDIEREGMQKADRIIA
                     VSEFTKRVIVEKYGIHPDKITVVHNAVYPIGEGEKRNLILFLGRLTIQKGPEFFLKTA
                     KKVLDYEDARFVIAGIGDMLPRLIEQAVHLGISNKVIFTGKLTEEEVKHIYKISSIYV
                     MPSVSEPFGITALEAISAGTPTIVSKSAGVSEVLKNCFKVDFWDTDEMANKIIALLRY
                     DSLRDTMTQSSKREIQLFTWDRVAERTLQVYRGLV"
BASE COUNT          881 a          787 c          724 g         1037 t
ORIGIN      
        1 ccatagcttc gtcgtagcgc aagtcggtct tcgttcgaca cttgtgcagg atcgtatttt
       61 gcggggtcga ctccgttcca gcagggacgg attttttctt gcgggaaacc aagtttcaga
      121 agttcgtctg ccatggcatt ggagactgtg atgacacaat ccgctatttg gcctccttca
      181 tattcaatat cctttatcgt atgagatcct cttccaatcg atcgtccgac ctctgtagaa
      241 tgcacatgga aaattaaggg gatgttcagt tctttttttg cgaccatccc tgccatgatc
      301 ccaagccagt cgtgtgcatc gatgatatca aatgtacgcc cattcttgcg aacaaggacg
      361 ttgaccagct ttgacgcaga catgatattg tagctgagga catcagcgaa aaatgtaaaa
      421 tacggtcccc aggagcggag ctcatggtcg gcacagaggt aaaacggttt gcttagatca
      481 agtgtttttg gtcgatacac gtcgacgccg ttgatctgat cggaggtagg aagtgtattc
      541 tgccggttca gggtgaaaac cgtcacatca tgacccaatt ctacctgctt ttttgaaatc
      601 tcagtcgcaa atgtgccaag tccaccatgg ataactggtg gatactccca cgaaaaatgc
      661 acgatcttca tggaatgttt tgtctcctca ccgattttct gctttttctt ttaggtcttg
      721 caggatgttc atgtaattaa taaacccatc gtacgggatg tcatacggac tgaaatacgc
      781 gtgaacgttc ccatcctcaa aacctttcgt cgagatgtaa tacaagtgat ctgatgtctg
      841 gagtaatctc cagatgtaga ggagatcccg atctcctttt cgtttcagtt tccgtccgat
      901 gtctttgagt tcgttgaagc aagcaatctg catgttattg cccagccagg tggaaacatt
      961 tctatcctcg tctgcccagg agatcgccca gggggaatcg acgtcgccga ccgcgttgta
     1021 ccgttcgact gcctcgctta cggtggcaaa atcaagggaa tcgtgtttca gtacctcacc
     1081 ggggagatgt ttgaggaatt caaaaatccc tgtttctatc cattgatgtt ccccgaaggt
     1141 ctcgtaatcc atgaaaaggt tgatgacatc tccatatgaa ttggacatcc agtttgcata
     1201 tttatctgcg gtaaggggaa aacctggcca attccgagcg gagaatcgga acccgacatc
     1261 atcgctgagt ttatagttac gtagcaggac cttcaggttc gcattggcgg ctttataaat
     1321 gaagttcggt gagcgccatc ccaggatttt ctcggctcct tccgtaagaa tacctttata
     1381 tccaaggtca gatatttttc gtgcgatcct attatcgtag atggcttccg tgtttcggaa
     1441 cacctttggt ttgtaattga aaatccgtcg gatcatctgt cgatgcatct ttatctggtc
     1501 ttcgaactcg tcaagctcgt catacagccc tgagagagaa tgaaaatagg tttcctcaat
     1561 gagatcgaca gcaccagtct tgaagagttc cttaaaacta tcaaggagtt caggaagata
     1621 attctcgcag tattcaacga acgtcccagt cagactatag gagatgcgga atttcccgtc
     1681 gtactttcgg atcaattcaa ggagcagatt attcgtgggg agataacatt tgtttgcgac
     1741 cttttcaaag atcttctggt tgaggtgttt atcaaaatac gtagaatgaa tgtcttttgt
     1801 atttccgata ttgaacacag aaaaatgatt caacctaaat ggttggtgca cctcgaagta
     1861 gaaacatatg gacggcattt tagactaagc ccctgtagac ctgaagcgtt cgttctgcga
     1921 cacggtccca ggtaaataat tgtatctccc tttttgaact ttgcgtcatc gtgtcacgca
     1981 atgaatcata gcgtagcagt gcgataattt tgttcgccat ctcatccgta tcccagaaat
     2041 ccaccttgaa acagttcttc aacacctccg agacacccgc gctttttgac acaatggttg
     2101 gtgtccctgc ggagatcgct tcaagggcgg tgatcccgaa cggctcactc accgatggca
     2161 tcacgtagat gcttgagatt ttgtagatgt gtttgacttc ctcttccgta agtttccctg
     2221 taaaaatgac tttattcgag atacccagat gaacagcttg ttcaatcaaa cgagggagca
     2281 tatccccgat ccctgcaatc acgaacctgg catcctcgta atccaagact ttttttgcag
     2341 tttttaggaa aaactctggg cctttctgga tcgtgagtct tcctaggaac aggataagat
     2401 ttcgtttctc tccttcacct atcggataga cggcattgtg caccacggtg attttatccg
     2461 gatgaatccc gtatttctcg acgataaccc gtttggtgaa ctcactgact gcgatgatac
     2521 gatccgcttt ttgcatcccc tctcgttcga tatcgatgaa ccattggttt ggatagatcc
     2581 atccagacct gtcatattcg gtcgagtgaa ccgtcaatac aagggggacg cctaattttt
     2641 cttttacagc tatcgctgct ttcatcgtca gccaatcatg acaatggatg acatcgtatt
     2701 tcccttttat ccgtttcact aataaatcat tgtatcggta caccgcatca aagaatttcc
     2761 caccaatctc tttgtcatcg ggccgatcgt agggataaac ctctgattca ccgacttcga
     2821 tgatcgtaat attcttgtta tcactcttga ccttctgtcg aagcttcggc atatagaagt
     2881 ctatatgaac atttttatcg gcaagaccat gggtcaaccc ataacagtgg gtacctaaac
     2941 cgcccacttt gaatggtggg tattcccagc cgatcattgc aattttcatt cctcttgtcc
     3001 tcaccaatta gcactaattg ttttcctgat aatctctact cccaaattcc ttttcgcatt
     3061 cctcgagagt ggacgataat tgaactgttt ttgatatgtt ccttgatttt taaccgttaa
     3121 acctacctca ttgttataag cttttcgttt ttatctacgt tcatttttct taaatctcgc
     3181 gaagaattta cccccgaagt caaaaatgac ttttatattt ttcgtctgtg aatgatatgc
     3241 tttagcagga tataatgtta tctgtaaaac actgaaaaaa agagtgaaaa ataagtagag
     3301 atttcaaata attaataatt cattttcgta tgatttctga ataaatgttg tcgctgtcac
     3361 ccaacacaaa aaaatagaat gcattgggtg tggtgacaca gcatcctcca cagtaagcag
     3421 cagcgggag
//
LOCUS       DATMXJ010000031         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_63207, whole genome shotgun sequence.
ACCESSION   DATMXJ010000031 DATMXJ010000000
VERSION     DATMXJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63207"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VN377_00905"
     CDS             complement(<1..724)
                     /locus_tag="VN377_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HWR26975.1"
                     /translation="MNQQQIKKEIETHHIRWIQIHFTDLMGGLRVFHVPAVRFLKDDV
                     LSKGSRFDGSSVGFRKVERSDMIAVPDPDTFLILPHLEGEALIRANLCDVDYNLYRAG
                     PRHILQRAVDLTKNLGYDSILISPEVEFYAFNHNESKLTEIKENQGYFIAPPLDNAKD
                     YRKKLSDALMDCGYQVRYHHHESGKSQHEIEISDLDAIPAADFCCFFKYIARDIATTY
                     NFDITFMPKPFSNEAGSGLHAHI"
     gene            827..1657
                     /locus_tag="VN377_00910"
     CDS             827..1657
                     /locus_tag="VN377_00910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWR26976.1"
                     /translation="MVEINKIYCMDCLQGLIKLHDTSVDVIVTSPPYNIGKPYRSYND
                     VRPRDEYLDWMETIAKKCLSKLKDQGSFFLNMGGKPTDPWIALDLANRFHNHYTLQNT
                     IHWIKSIAIPKEDVGNYAAITDDIAVGHYQPVNSTRYLSNCHEYIFHFTKTGNVPIDQ
                     LAIGVKYQDKTNIGRWKKASQDLRARGNTWFIPYETIQTARSHPTVFPVKLPEMCIRL
                     HGIRQNMLVLDPFMGTGSTALACQKLGVDYIGFEIDKTYVTIAKKKLLEQEKELSLLR
                     "
     gene            1717..>2009
                     /locus_tag="VN377_00915"
     CDS             1717..>2009
                     /locus_tag="VN377_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR26977.1"
                     /translation="MKKITPWLRDSTYIAPLSPACRMCAKGEKMVVLVTGLCSTKCFY
                     CPLSFKKGGTDRIFADEWELDNERDTEKLVREAEYIDAAGAGITGGDPLVVWQR"
BASE COUNT          576 a          455 c          453 g          525 t
ORIGIN      
        1 cgatatgggc gtgcaggccg ctccctgctt cattggagaa tggtttgggc atgaaggtga
       61 tgtcgaaatt atacgtcgtt gcgatatctc gagcgatgta cttgaagaag cagcagaagt
      121 cagctgcggg gattgcatca agatcggata tctcaatttc atgttgtgat ttccctgatt
      181 catgatgatg gtatctcacc tgatatccgc agtccatcag cgcatcagaa agtttcttcc
      241 gatagtcttt agcgttatca agcggtggcg cgataaaata cccttggttt tctttgattt
      301 cagtaagctt actttcgttg tgattgaagg cataaaactc gacttctggg gaaataagaa
      361 tgctgtcgta ccctagattc ttcgtaaggt ccactgctcg ctggaggata tgtcgaggtc
      421 ctgcgcggta caagttataa tcgacatcac agaggttcgc tctgatgagc gcttctcctt
      481 caagatgtgg aagaatcaag aaggtatcag gatcggggac cgcgatcatg tctgatcgtt
      541 caaccttccg gaacccaact gaggacccgt cgaaccgtga gcctttactc aggacatcat
      601 cttttaaaaa acgaactgcc gggacgtgga agacccgcaa tccccccatc agatctgtga
      661 aatggatttg gatccatctg atatgatgcg tttcaatttc tttttttatc tgttgttggt
      721 tcatgcgaac attggaaaca gtacgtatta taaatttttt atggatatcg ttacttacag
      781 caataaagaa aaaaagacaa agaaacagtc tttatgagag tcaacgatgg tagaaattaa
      841 caaaatctat tgtatggatt gtcttcaagg tttaatcaaa cttcatgata catccgtcga
      901 tgtcattgtg acctctccac cctacaacat tggaaaacca taccggtcgt ataacgatgt
      961 tcgaccacga gacgaatatc tggattggat ggagacgatc gcaaaaaaat gcttgtcaaa
     1021 gctaaaagat caaggatcgt ttttccttaa catgggcgga aaaccaactg acccctggat
     1081 cgcattggac ctcgcgaacc ggttccataa ccactatacg ctccagaaca cgattcattg
     1141 gattaaatcc attgcgattc ccaaagaaga cgtcggaaat tatgcagcaa tcaccgatga
     1201 catcgctgtc gggcattacc agccagtcaa cagcacacgg tacctcagca actgtcatga
     1261 gtacatcttc catttcacaa aaacagggaa tgttcccatc gaccaacttg ccattggagt
     1321 gaaataccag gataaaacaa atatcggtcg gtggaaaaaa gcatcacagg atctgcgagc
     1381 gagaggcaac acctggttca tcccctatga gacgattcaa acagcacgat ctcaccccac
     1441 ggttttccct gtgaaactcc cggagatgtg tattcgactt catggaatca gacagaacat
     1501 gctggttctt gacccgttca tgggaaccgg atcgactgca ctagcctgcc aaaaacttgg
     1561 agtggattac atcggttttg aaattgataa aacctacgtg acaatagcga aaaagaaact
     1621 cttagaacaa gaaaaagaac tctccctcct tcgataaatc ctattgtgtt ctatcatgta
     1681 tcttttaata cctctgaagc atagtggaat acgaccatga aaaaaatcac tccctggctc
     1741 cgtgacagta cctacatcgc tcccctctcg ccagcctgtc gcatgtgcgc gaaaggcgag
     1801 aaaatggtgg ttcttgtcac cggactttgc tcgacgaaat gtttctactg ccccctcagt
     1861 tttaaaaaag gcgggaccga taggatattt gccgatgaat gggagttgga caatgaacgt
     1921 gatacagaaa aactggttcg tgaagctgag tatatcgatg ccgctggtgc tgggatcact
     1981 ggcggggatc ctcttgttgt ctggcaacg
//
LOCUS       DATMXJ010000032         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10958, whole genome shotgun sequence.
ACCESSION   DATMXJ010000032 DATMXJ010000000
VERSION     DATMXJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10958"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1332)
                     /locus_tag="VN377_00920"
     CDS             complement(118..1332)
                     /locus_tag="VN377_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWR26978.1"
                     /translation="MTDTIDQSTVEIITDSLKQAESIDAHAVILLLDTPGGGLDETFQ
                     IAEMIKQSNIPVIGYVFPSGATAWSAGTFILLSTPLAAMADHTIIGSCQPIETSIEGT
                     RFINDSKIINALVEWLQERAVMYGRNETLARLFITENRNVNATVAKTSNVIEITASSV
                     DQLLNDIDGRNVTTASGPVTLHTKDAEQIRYSPSIQIQLLKLISNPILTSLLLMLGIF
                     ALLIGISTPGYGAEVFGVIAILLSLIGSGFSISTLAIIFIIIGCLLLVVEIFVLPGFG
                     AVGVGGIICLIIGSIFLIPSYPTRKWLISGEYMTDALIIMLIVIGLFALFFAFLLYKV
                     LQIRKKKPSLGKFIGEQAVAIEQLSPMKTGFVRFKGEYWQAKSDTVIETNTKVVIVDK
                     DETILIVKPVER"
     gene            complement(1428..2207)
                     /locus_tag="VN377_00925"
     CDS             complement(1428..2207)
                     /locus_tag="VN377_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWR26979.1"
                     /translation="MVDVNTMVIALFIIVIFLIAILASAIKIMPEYQRIVIFRLGRLL
                     GIKGPGLVFIIPIIDKPIRMDLRTRVIDVPKQRIITRDNVTVDVDAVVYFRITDPQKA
                     IIEVQRYDMATSLLAQTTLRDILGQKNLDELLSQREELNKSLQSILDNGTDPWGIKVS
                     AVTLRDVSLPEEMLRAIAKQAEAEREKRSRIIMAEGELLASAKMTEAAKLYEKTPIAL
                     RLRELQTLTEIAREKNLIIVPGGEIGTMAGLAKAVNKKAEQ"
BASE COUNT          613 a          522 c          528 g          715 t
ORIGIN      
        1 attcccgact tcgtcgctgc caccaagaaa atgacattta ataccagaat ccattttaca
       61 tcaaaccttt atacaatcag agcctcctaa agaaaaaaga ggttgattaa cttttggtca
      121 tctctccaca ggcttcacaa tcaggattgt ttcgtcttta tcgacgatga ccactttcgt
      181 atttgtttca atcaccgtgt ctgatttcgc ctgccagtat tcccctttga accgtacgaa
      241 gcctgttttc atcgggctga gctgttcgat ggcgactgct tgttccccga tgaattttcc
      301 tagtgatggt tttttctttc gtatctgaag tactttgtac agcaagaacg caaagaagag
      361 tgcgaagagt ccgatgacga tcagcataat gatgagtgcg tctgtcatat attcacctga
      421 tatcaaccat tttcttgttg ggtaacttgg aatgagaaat atcgatccga taataaggca
      481 gatgatacca ccgacgccga cagcaccaaa gccaggtagg acgaatattt ctacaacgag
      541 gagtaggcat ccgataatta taaagattat ggctagcgta gaaatgctaa atccagaccc
      601 gatgagcgaa aggagaatcg cgatgacgcc aaacacctca gcaccatatc ctggggtcga
      661 gattccaatg agcaacgcaa aaatcccaag catcaacaac aacgaagtaa ggatgggatt
      721 tgaaatgagt ttcaacagtt ggatttgtat cgacggggaa taccttatct gttccgcgtc
      781 ttttgtatgt aaggtgactg ggccgctcgc agtcgtgacg tttctaccat caatatcgtt
      841 caacagctga tcaacactcg atgcggtgat ttcgatgacg ttgctggttt ttgctacagt
      901 cgcgttgaca ttcctgtttt ctgtaataaa gagtctggcg agtgtttcat tgcgaccata
      961 catcaccgct cgttcttgga gccattcgac gagtgcgttg atgattttcg agtcattgat
     1021 gaatcgtgtt ccctcaatgc tcgtttcgat aggctggcac gatccaataa tcgtatgatc
     1081 tgccatggct gcaagcggtg tgctgagcag gataaatgtt cctgctgacc acgcggttgc
     1141 tccgctcgga aagacatatc cgatgaccgg gatattgctt tgtttgatca tctccgcgat
     1201 ttgaaaggtt tcatcaagtc cgcctccggg tgtatccaaa agaaggatta ctgcgtgagc
     1261 gtcgatggat tctgcttgct tcagactatc tgttatgatc tcaacggtcg attgatcgat
     1321 cgtatcggtc atttctacca gaagaacact cgacgtttgc gctaacgcaa tatctgaaat
     1381 aaaaaggaga atgaaaaaga aaaaaagtag gggagagtat ttgtatctca ttgttcagct
     1441 tttttattca cagcttttgc caatcctgcc atggtgccta tttcgccacc aggaactatg
     1501 atgaggttct tctcccgtgc tatctccgtg agcgtctgta actctctgag tcgtaaagcg
     1561 atcggtgttt tttcgtacag ttttgctgcc tccgtcattt tcgctgaggc aagaagctct
     1621 ccttctgcca taatgatcct tgatcgtttc tctcgctcgg cttctgcttg ttttgcgatt
     1681 gcccggagca tttcttccgg tagtgagaca tctcgtaatg tgacggctga taccttaatc
     1741 ccccaggggt cagtgccgtt gtctaagatg gattgaagac ttttgttcag ttcttctcgt
     1801 tgtgataaca gttcatcaag gttcttttga ccaaggatgt ctctgagtgt cgtctgtgca
     1861 agtaaactgg tcgccatatc atagcgttgc acttcaataa ttgctttttg cgggtcagtg
     1921 attctaaaat acaccacagc gtcaacatcg acagtcacat tatcacgggt gatgattctc
     1981 tgttttggga catcgatgac ccgtgttctc agatccattc gtatgggttt atcgatgata
     2041 gggataatga acacaagccc tgggcctttg ataccaagca atctccctaa tcgaaagatg
     2101 acgattcgtt ggtattccgg catgatcttt attgctgatg caagaattgc tatcagaaaa
     2161 atcacgatta taaacaatgc gataaccata gtgtttacat caaccataat atacgacctc
     2221 cagtgctgaa aaactcgatt ctgttcttaa gtgtttctgt agagcttttt tttgaaacga
     2281 tatttttaaa aaggtcgcta ccttatgatg tgcgaattgg cggggagccg aatcatgtct
     2341 gaaatactat cgataaaagc acgtgaggtt cttgattc
//
LOCUS       DATMXJ010000033         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_16322, whole genome shotgun sequence.
ACCESSION   DATMXJ010000033 DATMXJ010000000
VERSION     DATMXJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16322"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VN377_00930"
     CDS             <1..888
                     /locus_tag="VN377_00930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR26980.1"
                     /translation="TKEQMDSITDISETLLIPLYARAGETKSNNPLIDDRKAVEITEK
                     LNKIFATSDSPLYQRLARGKIRRTANKKLTAFLALRTRRFDRYCQEFLSRTTNGVIVE
                     LGCGLSSRFSRIDNGVVEWYDLDLPEVIAIREQFFQQTARSHMIASSVLDFQWMELID
                     PTKKNVLFIAEGLLMYLSESEVKSLVLEMQHRFPGCELVCEVENTFVINTLKKDRWKR
                     KFQLDHHLGPDVTMQFGIQEGKDFESWGGGIKFLDEWTIFDDHEKKLGWMNLFAFSKR
                     LRKAQWIVHYRLQSPKQSY"
     gene            complement(934..2301)
                     /locus_tag="VN377_00935"
     CDS             complement(934..2301)
                     /locus_tag="VN377_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR26981.1"
                     /translation="MKKIFIVAIIGLVLFTSVGATSYQNTPLSENQNQPLRTTYDMLC
                     IAPKVFSNSLQPLITHKNAVGVQTILLTTEDIYKNYDGRDNAEQIKYAIKDAKEQYNI
                     SYVLLVGGMKLLGSGWHVPVRYSLLDDGSGNTMFLTDLYFADLYKENGEFDDWDSNGN
                     GIFAEWGKDTLDLTPDVALGRLPCRTTKEVDNIVEKIITYETETYGSSWFNRMVAIGG
                     DSFPAYAGNEGEATCDVATSYMTGFTIQKLYTSTGNLTGPTDIINAVNQGCGFLMTRG
                     KGGTDRVRMGMPDGSELIAFQNKHVSKLSNKDQYPICVLGECIHGKFDVCLRNLIKVI
                     QNISGFTQYDCIYECIAWRLVREKDAGMIAVLTNTNICYGAPGDADENGIPDDAEMYG
                     GFLAVELFRLYSQDGIHTLGALHQQALSNYVDQFPVHTDKFHGKSVQEFILFGDPSLR
                     IGGYQ"
BASE COUNT          619 a          544 c          605 g          686 t
ORIGIN      
        1 accaaggaac aaatggattc gattaccgat atctcagaaa ccttgctcat ccccttgtat
       61 gcccgggcgg gagaaacaaa atcgaacaat cctctcatcg acgacagaaa ggctgttgag
      121 atcacagaaa aactcaacaa aattttcgcg acatccgatt caccgctata ccaacgactg
      181 gcaagaggaa aaatacgaag gaccgcgaac aagaaattaa ccgcttttct tgcgctgcgg
      241 acacggcggt ttgaccgata ctgtcaggag ttcctcagcc gcaccacaaa cggtgtcatt
      301 gttgagctcg gttgtggcct gagctcgcgt ttctccagga ttgacaatgg ggttgtggaa
      361 tggtacgacc tggacctgcc tgaggttatc gctatccggg aacaattctt ccagcagacc
      421 gcacgaagcc acatgattgc ttcgtccgtg cttgattttc aatggatgga actgatcgat
      481 ccgacaaaga aaaatgtgct tttcatcgcg gaagggttgc tgatgtacct ttctgaatct
      541 gaggtgaagt ccctagtcct tgagatgcaa catcgattcc ccggttgtga gcttgtctgt
      601 gaagtggaga acacgtttgt gataaacacg ctgaagaagg atcgatggaa aaggaaattc
      661 cagctggatc atcatcttgg cccggatgtg acgatgcaat ttggcatcca agagggaaag
      721 gatttcgaat cctggggtgg cgggatcaag ttccttgacg aatggaccat tttcgatgac
      781 catgagaaga aactggggtg gatgaacctc tttgcttttt ccaaacggtt acggaaagcc
      841 cagtggatcg ttcattatcg attacaatcc cctaaacaat cttactgaaa tccgaactat
      901 ttttttttta aaaaaaaaaa ggaaaaaaaa tggttattga tatccaccta ttcgtagact
      961 tgggtcgccg aagaggataa actcctggac gcttttacca tggaatttgt ctgtgtgcac
     1021 cgggaactgg tccacatagt tcgagagagc ctgttggtga agagccccga gtgtgtgtat
     1081 cccatcttgg ctgtataagc ggaacagctc cactgcaagg aatccgccgt acatctcagc
     1141 gtcatcaggg atgccgttct catcagcgtc accaggcgcg ccataacaaa tattcgtgtt
     1201 ggtcagcacc gcgatcattc ctgcatcctt ctctcgtacg agtcgccaag cgatgcattc
     1261 atagatacaa tcgtactggg tgaacccgga gatgttctga atgactttta tgagatttct
     1321 aaggcagaca tcaaatttcc catgaatgca ttcaccaagc acacaaatcg ggtattgatc
     1381 cttgttcgaa agttttgaga catgcttgtt ttggaatgcg attaattctg agccgtctgg
     1441 catgcccatt cggactcgat cggttcctcc tttgcctcgt gtcatgagaa atccgcatcc
     1501 ctggttgact gcgttgatga tatcggtcgg tccggtgaga ttcccggtgg aggtatacaa
     1561 tttctggatg gtgaatccgg tcatgtagct tgttgcgaca tcacaggtgg cttctccttc
     1621 gttgccagca taggctggga agctatctcc gccgatcgcg accatccggt tgaaccagct
     1681 gctgccgtag gtctcagttt cataggtgat gattttttcg acgatgttgt cgacttcttt
     1741 ggtcgtcctg cagggcagtc tccctaacgc gacatcaggg gtgagatcca gcgtgtcttt
     1801 gccccattcc gcaaagatgc cattgccgtt ggagtcccag tcatcgaact caccgttttc
     1861 tttgtagaga tccgcgaaat agagatcggt caggaacatc gtgtttccag acccatcgtc
     1921 tagaagggag tatcggactg gaacatgcca tcctgatcca aggagtttca ttccaccgac
     1981 caggagcacg tagctgatgt tgtattgttc ctttgcgtct ttgatcgcgt atttgatttg
     2041 ttctgcgttg tcacgaccgt cgtagttttt gtagatgtct tccgtggtca gtaagatggt
     2101 ttggacacca accgcgtttt tatgtgtgat gagtggttga aggctgtttg agaaaacctt
     2161 cggtgcgata cagagcatgt cataggtggt cctgagtggt tggttttgat tttcagaaag
     2221 aggggtgttt tggtacgagg tagccccgac gctagtgaac aacaccagcc caataattgc
     2281 tacgataaat atttttttca tctttttttt tcctcctttt ttatctacta gtagatagga
     2341 gtggagaata tattagagtt ttcatgaaaa agttggctaa acctacattt tagtacttgt
     2401 tttttctgcg aatgtaaaaa aattatgaaa aaaggacaaa ggagtcttta aaag
//
LOCUS       DATMXJ010000034        17968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_31994, whole genome shotgun sequence.
ACCESSION   DATMXJ010000034 DATMXJ010000000
VERSION     DATMXJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31994"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN377_00940"
     CDS             complement(<1..218)
                     /locus_tag="VN377_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWR26982.1"
                     /translation="MNGLDQRIQRHLRTNKKTHWHIDYLLPSTEIVDIFYKENTRKEE
                     CRIAREFERNFTNISGFGCSDCTCKSHL"
     gene            386..751
                     /locus_tag="VN377_00945"
     CDS             386..751
                     /locus_tag="VN377_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26983.1"
                     /translation="MESHNPQFISIESLSDARKEIKKIGSDPKSLDIMAPKAVFKVIK
                     LEQVILQDAIIIKQDMLSLGGEVAIPREAFELEKNPAAILIMGTLTQLKDLVGKLQRH
                     YPRIQTISAELAMVLEKIS"
     gene            808..2157
                     /gene="purB"
                     /locus_tag="VN377_00950"
     CDS             808..2157
                     /gene="purB"
                     /locus_tag="VN377_00950"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWR26984.1"
                     /translation="MENPVCPLDFRYGRKEIKDVFGETSKLQYLLDVEAALARAHAAV
                     GTISKKAADEISKKATVKYVTVKRVNEIEKETRHDIMAVTKALSEICSGDAGKYVHLG
                     ATSYDIVDTANALQFAAATDLIKAQIRELRVTLVGLAKEYKNTIMAGRTHGQFSIPIT
                     FGLKMAVYAMEVDRHLERLHECKSRLLVGKMSGAVGSGAAMGKHALKIQELVMADLKL
                     GTEEAATQIVGRDRYIELLSILANIASSMEKFATEIRNLQRSEIGEVSEAFEAKKQVG
                     SSTMPQKRNPILCEQVCGIARVIRSFIIPAYENAIQWHERDLCNSSSERFILPHSLIM
                     TDWIVFQMNKVFRHLQVNSERMRQNLENSKGLPMAEAVMTKLVEKGMGRGDAHELVRT
                     CSLKAVSDDKQLLSVLLENPKVVKLLKKKELEEVMNPRNYLGVSDKIVDQVVKKLTR"
     gene            2175..3329
                     /locus_tag="VN377_00955"
     CDS             2175..3329
                     /locus_tag="VN377_00955"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWR26985.1"
                     /translation="MDKLERLIGNTEEVITIEELRTVLDKPNPKAYIGFEPSGVVHLG
                     WKICTNKIKDFIACGFDFTVLLADWHAYINDKLGGDLEKIKLCGRYMQDCFAAMGVDT
                     SKVRFVYASDYVNDAKYWELVLKTSKATSVARVKRAMDIMGRAEDEADKDLSKLFYPA
                     MQVSDIFYLNLDVAYGGMDQRHAHMLARDVAKKLGLKPPIAVHTPLLTGLQAGSRMNP
                     TESPLKDIPPASQADIKKPDDYNKQLQSNPILGSTALPSDFKMSKSKPETMISIHDPP
                     DVVKKKLSKAYCPEKQIEGNPVLEVCRFVIFPDLEGKPFLIERPEKFGGNLQFSTYQE
                     LADAFTAGLHPLDLKNATAQHINKILEPVHRFFEAHPENLQKMKTAGILL"
     gene            complement(3376..6345)
                     /locus_tag="VN377_00960"
     CDS             complement(3376..6345)
                     /locus_tag="VN377_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR26986.1"
                     /translation="MMKNDKIIVVSGVAIIIFAAVGVYFWVPQKAEMQSVNTDDFIDI
                     TGDLKDVPGSITVSSSDPFYPLIATPLSVNYDEAGEQMVIPLYVMNFNNPSTAVARLQ
                     DQLTRSKNGETIKDDESAKNASLRIATDYWDHSEAALIIEYDEPGYNLGVMATPIASY
                     LRIPVIVTGEIDADVTKVLNDLGVKKTIICGRNLKGYGSVLKFNSVEEIVNASIIVVK
                     EKLKQEVDYITLTNPVDAWPPKVLDSKEFTFGPTKIQTGSMSQLGATVRGMVSGKSSV
                     TWSFTIPEDYKYALIKFEGINHDAENCEEMGDKVTFVMGPNLPDEPASLQKFEIYPGN
                     TAGGGIAERDASGNIIKDVLYSESVLYGRGGVEYNIVVSGSWMIKKEGEVSARVVVEK
                     LEDPVYPMMKSLSSVAPYLTAYHKGILFGKPEFAFTADDDKITDKGETCPGLYVGGKN
                     PKLVPISNKHFFDKIHDPLNGLLAYLAGITLNDDRDIKTLRDYYVNNSGVYIALVGDG
                     TVLPNYIYQNHVEPVGDPSGTGVDETPYYSGGGTPSDVLYGNIDPKRYDWSNLANDVY
                     SDSEEKYPYIENIVGRITGWDAQDADALILRSVFYNELTSDLNTWKDNFGLVVGGGQD
                     FQKPLVRYIIFGDILKTIKRGEPMKGFTGYAEMTYKRASYDIVEPLGFTLQGALYEEG
                     MAKGLSEETLNEIKTNNLVNRLLFEKWQIRRLVGEGNVKGGDVFENSNFLFVNGHGNQ
                     FQFGVAGNDIVASGIGGPLMKAFFTQTIVPILGGFIGPGGDLSTVGDYTTRTVSNMNM
                     GPSFMWLESCICGKLDGQYSKTNIAQSLVHAGVASLIASPTGSNIGGGYLEPKNTKYD
                     FPIQTYLKYIKAKGQWKRGIFPDAHFGEKLYEDLCTNMRKLNCSVGMALRDAKNSYLP
                     EDASWLLWWSPPLNIYTGDALLDLEIAKNNYADLYKTTAAKGLSPMMESKYVSFQEYI
                     LFGDPALNLYEPCNEGR"
     gene            6514..6891
                     /locus_tag="VN377_00965"
     CDS             6514..6891
                     /locus_tag="VN377_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HWR26987.1"
                     /translation="MPGEELSPQLQDQINRLQQARAQLQMISQQRQQVEIQLKETEEA
                     LKEIEAVTEKTPIYKSIGALLIKTKGKSDTKKELTSTKESLELRKTTLEKQEGRSRER
                     LTELQSKVESSLKLAGGTGPLNG"
     gene            6884..7183
                     /locus_tag="VN377_00970"
     CDS             6884..7183
                     /locus_tag="VN377_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26988.1"
                     /translation="MVKKHIFSAHNDMKTGFFCKFFLILGCIFLILYIVEVVTHPLNL
                     SNDMTGTILAFTILFIGLGLIAYFFSCQFAKLSKIAEEVENDESLMDTEETKEEP"
     gene            complement(7191..7961)
                     /gene="uppS"
                     /locus_tag="VN377_00975"
     CDS             complement(7191..7961)
                     /gene="uppS"
                     /locus_tag="VN377_00975"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWR26989.1"
                     /translation="MYHFFSLKVNDFLDVKLLEEVQRSPLPRHIAIIMDGNRRFAKEL
                     GLSWEAGHVFGKEKIEEVLDWCFDLGIRVLTVYAFSTENFKRSEKEVKTLMSLLKEEL
                     DLAKEDSRIHKNKVRVRILGRLESLPKDIQESAQSIMELTQSYKTYHLNIALAYGGRE
                     EIIQAIQHMGGDIKKGKLKVKDISETTVASYLYTSGLPDPDLILRTSGEERISNFLLW
                     QLAYSELYFCDVYWPALQKRDFLLAIRTYQQRKRRFGK"
     gene            complement(8220..8930)
                     /locus_tag="VN377_00980"
     CDS             complement(8220..8930)
                     /locus_tag="VN377_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26990.1"
                     /translation="MQKQVEMSHHQIDFLKSLDTEEFYNKDEDDVRRYAHNTIQELVE
                     LGNQATPGLIELLQTEFTWSCFFALAIFRETKDSSAVPALISFLKRESDDSMANEEAM
                     FALQDIGDPSINPLVEDIEEAFNNKKYNSYLVGALTGIIGPESYEFMMKITKDFIGKP
                     WRYKGWFRVEDFTYNFVKQGRIDVIPFLRQILDMKSITGSEKHELEDTIRALEDPVGY
                     EKDIEETEKELFDATKNA"
     gene            complement(8962..9885)
                     /locus_tag="VN377_00985"
     CDS             complement(8962..9885)
                     /locus_tag="VN377_00985"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HWR26991.1"
                     /translation="MNEIQYKTVSIGIFYFSGTGNTKAVTYLLAKEMENKGCHVDIYA
                     IDEVLKTKDNQSLNTYDVVGFGYPVHAFNAPRIFFEFVNRLPAGNQKKTFVFRSAGDP
                     FLNGGATALVRKALQNKGYDVCLERMFVMPANVLIRYRDPIIKHLYTTAVRHAKTMTT
                     DVLSGTQRLQSNTIISTALTAGFSSMERIGARFFGKHLTVVSSCNHCGICIKNCPTKN
                     ITEHASRILFGSQCTFCMRCIYGCPVQAIVPRFLKFLVIRPWFDLEKIGKNPSLQDTY
                     ITQATKGYFRHFHKYVSEGYPSRDADGQIFK"
     gene            complement(9974..11155)
                     /locus_tag="VN377_00990"
     CDS             complement(9974..11155)
                     /locus_tag="VN377_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR26992.1"
                     /translation="MNKQFFFQGFARNIIIVGFVSLFTDLSSQMVFPVVPLFLLSLGA
                     SAWVVGLVEGAAETTASLLKVFSGYWSDKIKRRKPFVFAGYGLSSLTKPLFAFAQSWP
                     FVLFFRVIERVGKGVRDAPRDAIIAESVDVSIRGKAYGFQRAMDGVGSFSGAIIAVIL
                     LPLFGYRDLFLIAFFPGIIAVLFILFIKEKREPTIPQKRSATPFRVSMKRLPRNLQIF
                     ILVSALFALANFGYAFLILKAKNIGFSDNVALLLYVLFYVVYTLFTIPAGMLSDRIGR
                     KPVLMFGYILFAVTAVCLLFTSQLSMLVLVFIVYGIFFGLIDGVQRAFVVDLAPSELK
                     GTALGTFHTATGLIALPAGVIAGSLWVAYSPQATFLFALGLSICALLLFFFVKNNKQH
                     E"
     gene            11270..11986
                     /locus_tag="VN377_00995"
     CDS             11270..11986
                     /locus_tag="VN377_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR26993.1"
                     /translation="MSAVEVKNLVKRYGSMDAVKGISFSIDEGEIFGLIGPNGAGKTT
                     TLRVLSTLLSINGGSVTILGHDLSKHAGEIRKLISYLPEDAGAYKNLSGRAYLQFIAN
                     FFDSKEAPTMVQKGIKITDLGTRIDDRVDTYSKGMMRRLLVGRALMTNPKLAILDEPT
                     SGLDVINAQEIRKLVKTTAQSGTTVLLSSHNMLEVEYLCDRIALISEGKIVEEGKPKT
                     LMEKYNARNIEEVFIRTVHE"
     gene            11983..13263
                     /locus_tag="VN377_01000"
     CDS             11983..13263
                     /locus_tag="VN377_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR26994.1"
                     /translation="MSGLGNITKKEIRELSTPATFVPIILVALIFATMGSSIQGIQEK
                     STAPPTIGVITEDTSTLGVYASTILHTHAKSVYNSTSASDLKNGLETVTQQNGVAVIV
                     IPRNFTERITQGQQGTLQVYWIMKGAGLLDTISSSAVESLIAYINTNISKELINQNSS
                     VNASFVLSPTQRLETTYFKDREFVGISPNTISGMLSSQSLLIPIVMMMIIIMAGSIVI
                     TSMALEKENKTLETLLTLPVKRTSIVSGKIIAAAFIGLILAIIYMVGMQFYFSGFQMT
                     TGVNLAAYGLVLTTTDFIWIGVSLFLALIAGLSLCMLLGTFAKNYKSAQTLTFPITML
                     AMIPMFITMFADYDTLPLAVKIFTFAIPFSHPMMAPRALLFHDYMLVIGGIVYVAVFA
                     IVTISIVVWVFKTDRLLTGTTKFKWLKKMKIRRF"
     gene            complement(13285..13641)
                     /locus_tag="VN377_01005"
     CDS             complement(13285..13641)
                     /locus_tag="VN377_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR26995.1"
                     /translation="MKTILKISSLALLLCIGFIPTSTGASFEPAKTVVYLGDNNPGNY
                     SLLQFAIQHGSNQDALSISHGTDYHGRILKGYSQLQNFDIVVVSHLMSAGYSYQVPFH
                     LIGVFFSDQFSADANL"
     gene            13802..15685
                     /locus_tag="VN377_01010"
     CDS             13802..15685
                     /locus_tag="VN377_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWR26996.1"
                     /translation="MKNHTHHSKSRIWEKMTGWKGIWFHLAGIIAIAWFLIRVVPKPQ
                     RAQYPCQQVAMSLSLGYIAFWGILFTGLAVWLRNAKTKFAKTIPTVFAGFVVLFTITG
                     AVFATNYFQAEQPAATSWDPIPKEPMGIGTGVSPGRVVWVWNPNATEKDLSGYWWEQQ
                     NNNQDVIDQMFSTGVQDFTGTSTDIEAWDAIFSYFNEVHGNGNVGYQPGEKIAIKMNL
                     NNALGGVGDPYTKEDNDRDASPYVIKALLRQLVNVVGVAQQDITVFDASRPIVNCLYN
                     RIYYETYPADPLIEEFSQIHYVDSLGGATGREQVVPSSTKMYFSDGTIRTLPTCVAEA
                     KYLINMPLLKRHPINMGVTLSGKNFFGSFIEQVSDIHTYHISGLTMGNAAPQVDLFAY
                     EHLGGKTLMYLGDATFATKVDHRTIAKFLMYPFNDDWTNSLFFSQDPVAIDSVMYDFL
                     LTEGTNPIEGAQNYLHQAAVPASNVYDPEHDGTYLSTSLGVHEHWDRTIDIFSSDRYS
                     GPAGNGIDYRTTGEEFVRPGVVFTTPQEKHLYLFGTDKGAFPLSLIIGKIDVEAQVNG
                     VTGAVEKIEFYKDNTLQFTDTEAPYVWTWNKFSFFSHTIKIIAYYDAVNTTSNQIKVW
                     KIL"
     gene            complement(15697..15771)
                     /locus_tag="VN377_01015"
     tRNA            complement(15697..15771)
                     /locus_tag="VN377_01015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15734..15736),aa:Arg,seq:tct)
     gene            complement(15783..15870)
                     /locus_tag="VN377_01020"
     tRNA            complement(15783..15870)
                     /locus_tag="VN377_01020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15832..15834),aa:Ser,seq:tga)
     gene            complement(15923..16237)
                     /gene="rpsJ"
                     /locus_tag="VN377_01025"
     CDS             complement(15923..16237)
                     /gene="rpsJ"
                     /locus_tag="VN377_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWR26997.1"
                     /translation="MPAQKARISLTSTESTKLEEVCTQIKMIAEKTGVALRGPVPMPT
                     KRLRVPCRKSPDGEGTETIDHWEMRIHKRLIDLDASDRALRQLMRIQVPDGVHIEIVL
                     KS"
     gene            complement(16287..17570)
                     /gene="tuf"
                     /locus_tag="VN377_01030"
     CDS             complement(16287..17570)
                     /gene="tuf"
                     /locus_tag="VN377_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HWR26998.1"
                     /translation="MAEKPHLNLVIIGHVDHGKSTLVGRILLDTGQFPAHMVEKFREE
                     AKAKGKESFALAWIFDQLKEERERGVTIDVAHKRFDTDKYYITIIDAPGHRDFVKNMI
                     TGTSQADAAILVVAAQHGVMAQTKEHMFLARTLGVKQMIVAINQMDATTPPYDKARYE
                     QVKADVHALAKSVGYKDEQLQFVPVSAFEGDNIKDKKGITWWTGDTILKAIDKFVLPE
                     KPVNLPLRWPIQDVYTIKGVGTVPVGKVECGIMKPGQTLVFKPSMKPGGVTGEVKTIE
                     MHHEMISQAMPGDNIGVNVRGLNKGDIKRGDVAGPANDPPTVAKSFTAQIMVLNHPSV
                     ITKGYTPVFHCYTAQVACRFEEILKKLDPKTGQVKEENPDFIKTGDAAIVKIVPTRPM
                     VIEPSKKIPQLGRFAIRDMGQTVAAGVCLEVEAVK"
     gene            complement(17724..>17968)
                     /locus_tag="VN377_01035"
     CDS             complement(17724..>17968)
                     /locus_tag="VN377_01035"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504130.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWR26999.1"
                     /translation="NEKKEPLMNLLAEEMKDHRDIINIDRTDGLKLYMNDGWVLMRPS
                     GTEPIFRVYVEAKEKHKAEQVATFYKNLVESLMQHA"
BASE COUNT         5098 a         4184 c         3887 g         4799 t
ORIGIN      
        1 aataaatgac ttttacaggt acaatcacta catccaaaac ctgagatatt tgtaaaattt
       61 ctttcgaact ctcgagcgat tctgcattct tcctttcgag tattttcttt ataaaatatg
      121 tcaacaatct ctgtggaagg aagaagataa tcaatatgcc aatgggtctt tttgttcgtc
      181 ctgagatgcc gttgtattcg ttggtcaagt ccattcaacg cagacccaac ataggcatag
      241 catccttttt gaaaatgaac gacccccctg tttccctacc atgatcgagg cgtcattgcg
      301 aagttccatg accaggagat aggtccctct catcatagtt gtatgagcat aggttcttat
      361 aagaccgatt ctgtttcctc ctctgatgga atcgcataac cctcagttca tttccattga
      421 atccctttct gacgcaagga aggaaatcaa aaagatagga agcgacccaa agagccttga
      481 catcatggct ccgaaagcgg tttttaaggt cataaaactt gagcaagtga tattacaaga
      541 cgcgatcatc atcaaacagg atatgctttc ccttggagga gaggtcgcga tccctcgcga
      601 agcatttgaa ctagagaaaa accctgccgc tattttaatc atggggacgt tgacccaact
      661 gaaggatctg gttgggaaac tccagcgtca ttacccgcgc atccaaacaa tctctgctga
      721 acttgctatg gtattggaaa aaatctcata agcaagcatg tcccaaaaaa gacattaact
      781 catagatgat tctaggtcag aagccttatg gaaaaccctg tttgtcccct tgatttccgc
      841 tatgggagaa aagagataaa agacgtcttt ggcgagacaa gcaaactaca atacctttta
      901 gatgtcgaag ccgcactagc gcgagctcat gctgctgttg gtaccatttc aaagaaagct
      961 gcagatgaga tttccaagaa agccactgta aaatacgtca cagtaaaacg agtcaatgaa
     1021 atcgaaaaag agaccagaca tgacatcatg gcagtcacta aagcactaag cgaaatatgc
     1081 agcggtgatg caggaaaata cgtgcatctg ggtgcaacca gctacgatat cgttgatacc
     1141 gcaaatgcct tacaatttgc agcagcgacc gatctcatca aagcacagat acgagagctg
     1201 cgggtgaccc ttgttggtct ggcaaaggaa tacaagaaca cgatcatggc aggaaggacc
     1261 catggacaat tctcaatccc tattactttt ggtttgaaga tggcggtcta cgccatggag
     1321 gtcgaccggc atctggaacg actccatgaa tgcaaatcac gcctgctggt cggcaagatg
     1381 tctggagcgg tcggctccgg ggcagcgatg ggcaagcatg cgttgaaaat ccaggagctg
     1441 gtcatggccg acctcaaact cggaacagaa gaggcagcaa cccagatcgt gggtcgtgat
     1501 cgctatattg agttactatc catattagca aatatcgcaa gcagcatgga gaagttcgca
     1561 acagaaatcc gcaatctcca acgcagcgag ataggcgagg tctccgaggc gttcgaagca
     1621 aaaaaacagg taggctcttc aacgatgccg cagaaacgca acccgatcct ctgcgagcag
     1681 gtctgtggga tcgccagagt catcagaagc ttcatcatcc cagcctatga gaacgcgatc
     1741 caatggcatg aacgggatct gtgcaactca tcatcagaac gtttcatcct accgcattct
     1801 ttgattatga cggattggat cgtgttccag atgaacaagg tgttccggca ccttcaagtg
     1861 aactcggagc ggatgcgaca gaacctggag aactccaaag gactgccgat ggctgaagcg
     1921 gttatgacga aacttgtgga gaaaggcatg ggccgaggcg atgctcatga actggtcaga
     1981 acctgctccc tgaaagcagt gagtgacgat aaacaactcc tttccgtgtt acttgagaac
     2041 cccaaggtcg taaaactcct taagaaaaaa gaacttgaag aggtcatgaa tccccggaat
     2101 tatcttggcg tctccgataa aatcgttgat caggtggtca agaaactcac gcgataaaaa
     2161 gaaggtgcaa gatcatggat aaactcgaaa gactcatcgg aaacactgaa gaagtcatca
     2221 ccatcgaaga gctcagaaca gtgcttgata aaccaaaccc gaaagcatat atcgggtttg
     2281 aaccctcagg ggtggttcat cttggttgga agatttgtac taacaagatt aaggacttta
     2341 tcgcctgtgg gtttgatttc accgtgctgc tcgcggattg gcacgcgtac atcaacgaca
     2401 aactcggagg agatcttgag aaaatcaaac tctgtggtag atacatgcag gattgttttg
     2461 cggcgatggg ggtcgatacc agtaaggtac ggttcgtgta cgcaagcgat tacgtcaacg
     2521 acgcgaaata ctgggaactg gtgttgaaga catcgaaagc aacatcggtc gccagggtga
     2581 aacgcgcgat ggatattatg gggcgagctg aagatgaggc agataaggat ctctcaaaat
     2641 tgttttatcc agcaatgcaa gtttctgata ttttctattt aaatttagat gtagcttatg
     2701 gaggtatgga tcaacgccat gcacatatgc ttgctcgaga tgttgcaaag aagctcggat
     2761 taaaaccacc tattgctgtt catacaccat tattgacggg tttacaggct ggttctcgaa
     2821 tgaacccgac tgaatctcct ttgaaagata tacctccggc gagtcaggca gacattaaaa
     2881 aaccagatga ttataataaa caacttcaat ctaaccctat tctgggtagc acagcattac
     2941 cttcagattt taaaatgagc aagagcaaac cagagacgat gatctcgata catgaccctc
     3001 ctgatgtcgt gaaaaagaaa ttaagcaaag catactgccc ggagaaacaa atcgagggaa
     3061 acccggtcct ggaggtctgt aggtttgtga tcttcccaga ccttgaggga aaaccgttct
     3121 tgatcgagcg acctgagaag ttcgggggga acctccagtt cagcacgtac caagagcttg
     3181 cagacgcatt cacagcagga ctccacccgc tggatctgaa gaacgcgact gcacaacaca
     3241 tcaataaaat tctcgaaccg gttcataggt tctttgaagc gcaccctgag aatttgcaga
     3301 agatgaaaac agcaggaatc ctcctataac aatactggta ttccatagga cgaaaaatga
     3361 cgagagaagg aagacttacc ttccttcgtt gcaaggttcg tacaggttta acgccgggtc
     3421 accaaagagg atgtattcct ggaaactgac gtattttgat tccatcatcg gacttaatcc
     3481 tttggctgca gtcgtcttgt acaaatcagc ataattgttc ttcgctatct ctaggtctaa
     3541 cagcgcatcc cccgtgtaaa tattcagagg gggtgaccac cacagcagcc aactcgcatc
     3601 ctccggtaaa tagctgtttt tcgcatctcg gagcgccatt ccgacactac agttgagttt
     3661 cctcatattc gtacagaggt cttcatagag tttctccccg aaatgcgcat ctgggaagat
     3721 gcctctcttc cactggccct tcgcctttat gtatttcaga taggtttgaa tgggaaaatc
     3781 gtattttgtg ttcttaggct caagatatcc tcccccgatg tttgaccctg tcggtgaggc
     3841 aataagagac gctaccccag cgtgtaccaa ggattgggcg atattcgttt ttgaatactg
     3901 tccatcgagt ttcccgcaga tacagctttc caaccacata aatgaaggac ccatattcat
     3961 gtttgatacg gtccgtgtgg tataatcacc taccgtgctt aggtcaccac cagggccgat
     4021 aaaaccaccg aggatcggca caatagtctg cgtaaaaaat gctttcatga gtggaccacc
     4081 aattcctgat gccacaatat cattcccagc tacaccaaat tgaaactgat tcccatgacc
     4141 attgacaaac aaaaagttgc tgttctcgaa gacatcccca cctttcacgt tcccttcacc
     4201 aacgagtctt cgaatctgcc atttttcaaa gagaagtctg ttgacgaggt tgttggtttt
     4261 aatttcgttt aacgtctctt cactcagccc ttttgccatg ccctcttcat ataatgctcc
     4321 ttgcaaggtg aatcctaagg gttctacaat gtcataggaa gcccgtttat aggtcatctc
     4381 agcgtaccct gtgaagcctt tcatgggttc tccccgttta atcgttttca gtatgtcacc
     4441 aaagatgatg tatctgacca atggcttttg gaaatcctgt ccgcctccaa cgacaagacc
     4501 gaagttgtct ttccaggtgt tcaaatcgct tgtgagttcg ttatagaaaa cgcttctaag
     4561 aatcaacgca tctgcatcct gagcgtccca accggtgatg cgacccacga tgttttctat
     4621 ataggggtat ttctcctcgc tgtcgctgta gacatcattt gccaaattgg accagtcata
     4681 tcgtttcggg tcgatattcc catagagaac atcactggga gttccgccac cgctataata
     4741 aggagtttcg tcaacaccag ttccactagg gtctccgaca ggttccacat gattttgata
     4801 aatgtaattc ggaagaacag tcccatcacc aactaacgcg atatacacac ctgaattatt
     4861 cacataataa tcccgtaatg ttttgatatc tcgatcatca ttcagggtaa ttccagcgag
     4921 gtacgcaagg aggccattca atggatcatg gatcttatcg aaaaaatgtt tgtttgagat
     4981 cggtacgagt tttgggttct ttccaccaac ataaagacca gggcaggttt ctcctttatc
     5041 ggtaatctta tcatcatcag cggtaaacgc aaactcaggc ttcccaaaaa gaatcccttt
     5101 atgatacgca gtgagatagg gagcaaccga tgaaagactt ttcatcatcg gatagaccgg
     5161 gtcttcgagt ttttcgacaa caactcgagc agatacttcc ccttcttttt ttatcatcca
     5221 actcccggaa acaacaatat tatattcaac cccacctcgt ccatacaaga ccgattcaga
     5281 atagaggaca tctttgatga tgttaccact tgcatctctt tcagcgatgc cccctcctgc
     5341 ggtatttcct ggataaatct caaatttttg gagactggca ggttcatccg gtaaattagg
     5401 acccatcaca aatgtgacct tgtcccccat ttcctcacaa ttctcagcat catggttaat
     5461 accctcaaat ttaatcaagg catacttata atcctccggg atggtgaaac tccaggtgac
     5521 ggagcttttc ccgctcacca taccacgaac cgttgctccg agttgactca tagagcctgt
     5581 ttgtattttt gtaggcccaa aggtgaattc cttactatct aaaaccttcg gaggccaagc
     5641 atccacagga tttgtaaggg tgatataatc gacttcctgc ttgagttttt ctttaacgac
     5701 tattattgat gcattgacta tctcctcgac cgagttgaat tttaagacag agccgtatcc
     5761 cttgagattt ctaccgcaga taatcgtttt tttcacacca aggtcgttga ggactttggt
     5821 cacatcagca tcaatttctc cggtcacgat cacaggtatc ctcagatagg aagcaatcgg
     5881 tgtcgccatc acaccaaggt tgtatccagg ttcgtcatat tcaatgatta acgcagcttc
     5941 actatgatcc cagtagtcgg tcgcaatccg taacgaagcg ttttttgcgg attcatcgtc
     6001 tttaattgtt tcaccgtttt tactgcgagt taattgatcc tgcaatcgtg ccaccgctgt
     6061 tgacgggttg ttaaaattca tgacgtacag cgggatgacc atctgttccc cagcttcatc
     6121 atagttcact gacaacggtg tcgcgataag cggataaaaa ggatcgctag aagaaactgt
     6181 gatagaacca ggtacgtctt ttaaatcacc agtaatatcg ataaaatcgt ctgtattcac
     6241 tgactgcatt tctgcttttt gtggaaccca aaaatacact ccgaccgctg caaaaataat
     6301 aatagctacc cctgagacga cgatgatttt atcatttttc atcattattt aatcctccag
     6361 taaggataat aatatgtgtc tttgtatatt tacaacattc tatataatag tttctattat
     6421 taataggaag gggttttgta gtaaaacatc tggtgaatga atacgtttaa aaaggagact
     6481 attattacca cccacgaaag gagaaaaaac agcatgccag gtgaagaatt atcccctcaa
     6541 ctacaggatc aaatcaacag gttacagcaa gcacgagcgc aacttcagat gatttcacaa
     6601 cagcgacaac aggtggagat ccaactgaaa gaaaccgaag aagcattaaa ggaaatcgaa
     6661 gcggtgacag agaaaacacc tatctacaaa agcatcggtg ccttactgat taaaaccaaa
     6721 gggaaaagtg acacaaaaaa agaattaaca tcgacgaaag aatcactgga gttacgaaaa
     6781 accaccttgg aaaaacaaga gggacgaagc agagaaagac tcaccgagtt acaaagcaaa
     6841 gtagaaagtt ccttaaaact agcaggtgga acaggaccac tcaatggtta agaaacatat
     6901 tttttcagca cataatgata tgaagacagg gttcttctgt aaattttttc tcatcctcgg
     6961 atgcatcttt ttaatcctct atattgttga ggttgtcacc catcctctca acctgagcaa
     7021 cgatatgacc ggtacgatcc tagcattcac gattctcttt ataggcttag ggttaatcgc
     7081 ctattttttc tcctgtcaat tcgcaaaact ttcaaagata gcagaagaag tcgaaaacga
     7141 cgaatccctc atggacacgg aagagacaaa agaggaacca taaaacattt tcatttcccg
     7201 aaccgtcgtt tcctctgttg atacgtccgt atcgccagta agaaatcccg tttttgaagc
     7261 gcaggccagt acacatcaca aaagtagagt tccgagtatg cgagctgcca gagtaaaaaa
     7321 tttgagatac gctcttctcc ggatgttcga aggataaggt ccgggtctgg caacccactg
     7381 gtatacagat aggaggcgac tgttgtttca gagatatcct tgactttgag ttttcctttt
     7441 ttaatatcgc cacccatatg ctggatagct tgaatgatct cctctcggcc accgtacgcc
     7501 agcgcgatgt tcaaatgata ggttttatag gattgagtga gctccatgat ggactgcgct
     7561 gactcttgaa tgtcctttgg caaggattca agccttccaa gaatccgtac ccgcacttta
     7621 tttttatgaa tacgagagtc ttcctttgcc aaatccaact cttccttcag caaggacatc
     7681 aaggtcttga cttctttttc gcttcgctta aaattctcag tagaaaacgc atacacagtc
     7741 agcacacgta tccccaggtc gaaacaccaa tccagcacct cttcgatttt ttctttccca
     7801 aagacatgcc ctgcttccca ggacaacccc agttcttttg caaatcgacg gttcccgtcc
     7861 atgataatgg caatatgtct cggaagcgga gaacgctgta cttcctcaag gagtttgaca
     7921 tcgagaaaat cgttgacttt caaggaaaaa aaatgataca acgaagaacg atagaacgca
     7981 gcaagtaccc tcttcccagt tgaggttcga aggacgtatt tctcccattc ttcaatcttt
     8041 ttgccagcga gggccctcag tcttggtttt tgagaactaa tacagttccc tccagataaa
     8101 tcaataccgt tgccattata tagatttact gataatattc ttctcaatat aaaaatataa
     8161 tagatacgta tactaggact gtcgtcctct cttctttttg ttttttcagc tcgtctccat
     8221 caggcatttt tggtggcatc aaaaagctct ttttcggttt cctcgatatc cttttcatac
     8281 ccgaccgggt cctccagtgc tctgatggtg tcctcaagct catgtttttc actgccggtg
     8341 atactcttca tatcgagaat ttgccgaagg aaaggaatca catcaatccg accctgtttc
     8401 acaaaattat aggtgaaatc ttcgacacga aaccatcctt tgtatcgcca tggttttcct
     8461 ataaaatcct tggtgatttt catcatgaat tcatatgact ctggtccgat tatcccagtc
     8521 aacgcaccga ccaaatagga gttgtatttc ttattattaa acgcctcttc gatatcttcg
     8581 acgagggggt taatcgatgg gtctccgata tcctgcaacg caaacatcgc ttcctcattt
     8641 gccatcgaat catcagattc tctttttaaa aacgagatca acgcgggtac tgccgaagaa
     8701 tccttcgtct cacgaaagat cgctaaagca aaaaaacaac tccaggtaaa ttcggtctga
     8761 agaagctcaa taagtccagg ggtcgcttga tttcccaact caacgagttc ttggatggta
     8821 ttgtgagcgt atctgcggac gtcatcttcg tctttattat agaactcctc ggtgtccagt
     8881 gattttaaaa aatcgatttg atgatgagac atctcaacct gtttctgcat ggtacccctc
     8941 aaggaggtaa acagattcac gttacttaaa gatttgtcca tcagcatctc tcgaagggta
     9001 tccttcagaa acatatttgt gaaaatgtcg aaaataccct ttggttgcct gcgtgatgta
     9061 cgtgtcttgt aaggaggggt tctttccaat tttttcaaga tcgaaccacg ggcgtataac
     9121 taagaatttt aagaaacgtg gaacaatcgc ctgaaccgga caaccataga tgcaccgcat
     9181 acaaaaggta cattgagaac caaacaggat gcggctggcg tgttctgtta tattttttgt
     9241 tggacagttc ttgatacata ttccacagtg attgcaggag ctgaccacag tcagatgctt
     9301 gccaaagaaa cgagcaccaa tacgttccat actgctgaac ccagctgtca atgctgtgga
     9361 tatgattgtg ttgctttgca gcctttgtgt ccctgagaga acatcagttg tcattgtttt
     9421 tgcatgtcgt accgcagtgg tatacagatg cttaatgata gggtctctgt atcttatgag
     9481 tacgttggct ggcatgacaa acattcgttc taagcacaca tcatatcctt tgttttgtaa
     9541 cgcttttcta accagtgcag ttgcccctcc attcaagaac gggtcaccag cggatctaaa
     9601 tacaaatgtt tttttctgat tacccgcggg tagtctattt acgaattcaa agaaaattcg
     9661 aggtgcgtta aaggcgtgga caggatagcc aaaaccaacg acatcgtagg tattcaggga
     9721 ttgattgtct ttcgttttca gaacttcgtc aatcgcatag atatcaacat ggcatccctt
     9781 gttttccatt tcctttgcta agagatatgt tacggctttt gtgtttcctg ttcctgaaaa
     9841 ataaaaaatt cctatgctca ccgttttata ctgtatttca ttcacctgga aacctctact
     9901 acttgcatct ctcctgttag ttctatcgtc tgtttcttca taaagattcg cgtacgactg
     9961 ggctggttca tgtttattcg tgttgtttgt tatttttaac aaagaaaaac agtagcaaag
    10021 cacagatgga taaaccaaga gcaaatagaa aagttgcctg cggagagtat gccacccaga
    10081 gagatcctgc gataacccca gctggcaacg caattaaccc agttgctgtg tgaaaggtcc
    10141 ctagagcagt tcctttcagt tcggaaggtg ctaaatcaac aacaaaggcg cgttgtactc
    10201 catcgatgag cccaaagaaa attccataga caatgaagac caaaaccagc atcgaaagtt
    10261 gcgatgtaaa cagcagacag accgctgtga ccgcaaaaag gatatacccg aacataagga
    10321 caggttttct acctattcta tctgagagca ttcctgcagg gatggtaaat aaggtatata
    10381 ccacataaaa taagacatag agcagcagtg cgacattgtc agaaaaaccg atattctttg
    10441 cttttagaat gaggaaagca tatccgaagt tcgctaaagc aaataaagcc gagacaagaa
    10501 tgaatatctg aagattccga ggaagtcgtt tcatactgac gcgaaacggt gtcgctgaac
    10561 gtttctgagg aatcgtcggt tctcgtttct ctttaataaa gaggataaac aacactgcga
    10621 taataccagg gaagaatgcg atgaggaaga gatcccgata tccaaataac ggtaggagga
    10681 tcaccgcgat aatcgcaccg gaaaaagagc cgacaccatc catcgcccgt tgaaacccat
    10741 acgcctttcc tcgaatggag acatccacgg attcagcgat gatggcatct cggggggcat
    10801 cccgtactcc tttgcccacc cgttcgatca cccggaaaaa gagaacgaac ggccagctct
    10861 gtgcgaacgc gaaaagtggt tttgtcagag acgatagacc gtacccagcg aagacaaacg
    10921 gttttcgtcg ttttatttta tcggaccaat acccggagaa aaccttcagt aacgacgcgg
    10981 tggtttccgc ggctccttcg acgagcccta cgacccaggc gctcgcacca agcgacagta
    11041 agaataaggg aacaacagga aataccattt ggctggacag atcggtgaag aggctgacaa
    11101 aaccaacaat aataatattc ctagcgaacc cttggaaaaa gaattgtttg ttcatcgaat
    11161 acctcatggg agaaatgttg gtgtgcgaat gtattttcct cgtttcagtg tttcggtagt
    11221 acgaagagaa tagcttaaaa ggaaaaaaca attacgagga accgaaaaca tgtcagcagt
    11281 cgaagtaaag aatttggtga agagatacgg atccatggat gcggtgaaag gtatctcatt
    11341 ctcaattgac gaaggcgaga tcttcgggct tatcggcccg aacggcgcag ggaaaactac
    11401 cacattacgg gtattgtcca ccttgttgtc catcaacgga ggatcggtca ctatattagg
    11461 acatgatctg agtaaacacg ccggggaaat cagaaaactc atcagctatc ttccagaaga
    11521 tgccggagca tacaagaatc tgtcagggag agcgtatctt caattcatcg cgaacttctt
    11581 cgattcgaaa gaagcaccaa cgatggtgca aaaaggaatc aagattacgg acctcggaac
    11641 acggatcgat gaccgtgtcg atacctacag taaagggatg atgcgccgac tgttggttgg
    11701 aagggcttta atgacaaatc caaaactagc catccttgat gaaccaacct cgggccttga
    11761 tgtcatcaac gcccaggaaa tacgcaaact cgtcaagacc acggctcaat cagggacgac
    11821 cgtactgctc tcctcgcata acatgcttga ggtcgagtac ctctgcgaca ggatcgcttt
    11881 gatatctgaa gggaaaattg tcgaagaagg aaaaccaaaa accttaatgg agaaatataa
    11941 cgcacggaac atcgaggaag tctttatacg gacggtgcat gaatgagcgg acttgggaat
    12001 atcacaaaaa aagagatacg ggaattgtca acacctgcga catttgtccc tattatcctt
    12061 gtcgccttga tttttgcaac catgggaagt agcatccaag gcatccaaga aaaatcgaca
    12121 gcccccccta ccatcggggt catcacagag gataccagca ccttaggtgt ctatgcatcc
    12181 acaatcctgc acacccatgc caaatcagtg tacaactcca catcggcatc tgatcttaaa
    12241 aacggattag aaaccgtcac gcaacaaaat ggtgttgctg tcatcgttat cccaaggaat
    12301 ttcacggaac gaatcaccca aggacaacaa gggacgctcc aggtctattg gatcatgaaa
    12361 ggtgcaggac tcctggatac gatctcatca agtgccgttg aatcactcat tgcttacatt
    12421 aacacgaata tatcaaaaga actgattaat cagaattcgt cggtaaacgc ctcatttgta
    12481 ctttcaccga cccaacggct tgagacgacg tatttcaagg atagggagtt tgtcgggata
    12541 tcaccaaaca cgatatcggg catgctgtca tcccagtcgt tactcatccc gatcgtgatg
    12601 atgatgatca tcatcatggc aggaagcatc gttattacat ctatggcact tgaaaaagag
    12661 aataaaaccc ttgaaacgtt gctgacgtta cccgtgaaac ggaccagtat tgtctctggg
    12721 aagattatcg cggcagcatt catcggactt atccttgcca tcatctatat ggttggtatg
    12781 cagttttatt tctcaggttt tcaaatgaca actggggtga atctagcagc gtacggtctt
    12841 gttctaacca cgacagattt catctggatc ggtgtttcac tgtttcttgc gctcatcgct
    12901 ggtctatcgc tctgcatgct ccttgggacg tttgcaaaga actataagag tgctcaaaca
    12961 ctcacattcc cgatcacgat gctggcgatg atccctatgt ttatcaccat gttcgctgat
    13021 tatgacacgc tacctttggc agtgaagatc ttcacgttcg ccatcccatt ctcacatcca
    13081 atgatggcac cacgcgcgct tcttttccat gattatatgc ttgtcatcgg tggtatcgtg
    13141 tatgtcgctg tttttgcgat agtcaccatc agtatcgtgg tgtgggtgtt taagaccgac
    13201 cggctgctga caggcacaac aaaattcaaa tggttgaaaa aaatgaaaat aagacggttc
    13261 tagaaaagcc gtctcatttt ttttctacag attcgcatct gcagagaact ggtctgaaaa
    13321 gaacacaccg atgaggtgga acggtacctg atagctatag cccgcactca tcaaatggct
    13381 gaccacgacg atgtcaaaat tttgtaactg tgagtagcct ttgaggatgc gtccatggta
    13441 atcggttccg tgagagatac tcagtgcgtc ctggtttgaa ccatgttgta tggcaaactg
    13501 taggagacta taatttccag ggttgttatc tccaaggtat acgactgttt ttgccggctc
    13561 gaaacttgct cctgtcgagg ttgggataaa tccaatacat aacaggaggg cgagactgct
    13621 tattttcaaa atagttttca tttagtcacc tcttttatct taataaaatt gtaatacgat
    13681 gaccaatata aaattaacta ttgttaattc catgttattt ggagataaaa cataaggttt
    13741 ttcaacagcg agcaaacctt tttatagcct aacaactact ctttttgtaa ggggatatct
    13801 catgaagaat catacacatc attcaaaatc acgaatatgg gaaaaaatga ccgggtggaa
    13861 aggaatctgg ttccatctcg ccggcatcat cgctatcgcc tggtttctca ttcgggtagt
    13921 tccgaaacca cagcgcgcac aatatccttg ccagcaggtg gccatgtcac tatcacttgg
    13981 atatatcgca ttctggggca ttttattcac gggtcttgcg gtatggttgc gaaacgcaaa
    14041 gacaaaattc gctaaaacaa tcccaaccgt tttcgcaggt tttgttgtgt tattcaccat
    14101 cactggtgca gtgtttgcca cgaattattt ccaagcagaa caaccagccg caacatcctg
    14161 ggaccctatt ccgaaagaac caatgggaat cggaaccggg gtatcaccgg gtcgggtcgt
    14221 atgggtgtgg aacccaaatg caacagaaaa agatttatca ggatactggt gggaacaaca
    14281 gaacaacaac caggatgtca ttgatcagat gttctcgaca ggcgttcaag atttcacagg
    14341 aacatcaact gatattgaag catgggacgc tatcttttcc tattttaatg aagtccacgg
    14401 gaacggaaac gtaggctatc aaccaggaga aaaaatagcg attaaaatga atttaaataa
    14461 cgcacttggt ggcgttggcg atccctatac caaagaagat aacgatcggg acgcaagccc
    14521 ctatgtgata aaagcattgc tccgtcagct tgtcaatgtc gtgggtgttg cccagcagga
    14581 catcacggtg tttgacgcct cacgaccgat tgtgaactgt ctctataacc gaatctatta
    14641 tgagacctac cctgccgatc cgttaatcga ggaattctcg cagatccact acgttgactc
    14701 attaggggga gcaacgggtc gggagcaggt ggtgcctagc tcgacgaaaa tgtatttctc
    14761 cgatggtacc atccggacgc ttcccacctg tgttgccgaa gcgaaatacc tcatcaacat
    14821 gccattgctc aaacgacatc cgatcaacat gggtgtcact ttatcaggaa agaacttttt
    14881 cgggtcgttc atcgaacagg tctccgatat ccatacctat catatctctg gtctgacaat
    14941 ggggaacgcc gcaccgcaag tggatttatt cgcctatgaa cacctcggag gaaagacact
    15001 catgtaccta ggtgatgcga cgttcgccac caaagtggac catcggacca tcgcgaaatt
    15061 cctcatgtac ccgttcaacg atgattggac caacagcttg ttcttctccc aagacccggt
    15121 tgccattgac tccgtaatgt atgatttcct tcttaccgaa gggacgaacc cgattgaagg
    15181 ggctcagaat tatcttcatc aggccgcagt cccagcttca aatgtctatg acccggaaca
    15241 cgacggtacc tatctgtcga caagcttagg ggtccatgaa cactgggacc gaacaatcga
    15301 tatcttttcc tcagatcgat actcaggtcc tgcaggaaac ggcatcgatt atcgaaccac
    15361 tggagaagag tttgtccgcc caggggtcgt gtttacgacc ccccaagaaa aacatctcta
    15421 tctttttgga acggataaag gagcattccc cctctcactc atcatcggga aaatagatgt
    15481 ggaagcccag gtcaacggag tcacaggagc agttgaaaaa atcgagtttt acaaagataa
    15541 cacattacaa ttcactgata ccgaagcacc gtacgtctgg acatggaata agttttcgtt
    15601 cttcagtcat acgataaaaa tcatcgcgta ttacgatgca gtcaatacca caagcaatca
    15661 gattaaggtc tggaaaatac tttgatagca ttaaagcgga cgtggcggga ttcgaacccg
    15721 cgattaccga cttagaaggc cggtgcctta tccaggctag gccacacgcc caaagagagt
    15781 agcgctgagg gggagattcg aactcccgct ccccttgcgg agaaccggct ctcaaggccg
    15841 gcgcggtgga ccgagctttg ctacctcagc agcgttctaa ctaatgcaag ggtataggat
    15901 attcttataa aaaagggttg ttctatgatt tgagaacaat ctcaatatga accccatcag
    15961 ggacctggat cctcatcaac tgccgcaacg cacggtcact tgcatccaga tcaatgagcc
    16021 gtttgtgaat acgcatctcc caatggtcga tggtttctgt tccttcccca tcaggtgatt
    16081 tgcgacaagg aacccgcaac cgtttcgtcg gcatcggcac cggtcctcgc aacgcgacac
    16141 cggtcttttc tgcaatcatc ttaatttgcg tacacacttc ctcaagcttt gttgattccg
    16201 tgctggtcaa tgaaattcgt gctttctgtg ctggcatatt cggttgcccc catctattaa
    16261 aataataaaa agaagagagg agtaaattac tttaccgcct cgacttctaa acagacacca
    16321 gctgcgacag tctgccccat atctcgaatg gcgaatctcc caagttgcgg gattttcttt
    16381 gacggttcga taaccatcgg acgggtgggg acgatcttga cgatcgcggc gtcacctgtt
    16441 ttaatgaaat cagggttctc ttctttgact tgcccggttt tcggatcaag tttctttagg
    16501 atttcttcaa acctgcatgc gacttgcgcc gtgtaacagt ggaagacagg cgtgtatcct
    16561 ttcgtgatga cggacggatg gttcaacacc atgatctgcg cggtaaatga cttagcaaca
    16621 gttggcgggt catttgctgg tccagcgacg tctcctcttt tgatatctcc tttgttcaga
    16681 ccgcggacgt tgacaccaat gttgtcacca ggcattgctt ggctgatcat ctcatggtgc
    16741 atctcaatgg tctttacctc tccagtgacc ccaccgggtt tcatactagg tttaaagaca
    16801 agggtctgtc caggtttcat gataccgcat tcgacctttc cgacaggaac cgtaccgaca
    16861 cccttaatgg tgtagacatc ctggatcggc catcggagcg ggagattcac tggtttttca
    16921 ggaaggacaa atttgtcaat cgctttcaga atcgtatcac cagtccacca ggtgattcct
    16981 tttttatctt ttatgttgtc gccttcgaaa gccgagacag gtacgaattg gagttgctca
    17041 tccttataac cgacgctttt tgctaaagca tggacgtccg ctttcacctg ttcataccgt
    17101 gctttatcgt aaggcggtgt ggtcgcatcc atctggttaa tcgcaacaat catctgtttg
    17161 acaccgagtg tccgagcaag gaacatgtgc tcctttgtct gtgccatgac accatgctgt
    17221 gcagcaacca caaggatagc ggcatcagct tgggatgtac cggtgatcat gtttttaacg
    17281 aaatcacggt gaccaggggc atcaatgatt gtgatgtaat atttgtcagt atcaaatcgt
    17341 ttgtgcgcaa catcaatagt gactcctcgt tctcgctcct ctttcaactg atcgaaaatc
    17401 catgcgagag caaaactttc ttttcctttt gcttttgctt cctctctgaa tttctcaacc
    17461 atatgcgctg ggaactgtcc ggtatcaagt aagattcttc cgacaagtgt ggatttgcca
    17521 tgatcgacgt gaccaataat tactaaattt aggtgtggtt tttcagccat gattttacct
    17581 ccatgagtct acattttcca ttcagtattt atcttttcct attcctttct ttacttcgaa
    17641 ggtttgatag cgtatagcct tcttatttat aagttttacc tagatgacag gagacgcact
    17701 accacatgga aaaaaataat tctctacgca tgttgcatga gggattcgac aaggtttttg
    17761 tagaaggtcg ctacctgttc agccttgtgt ttttcttttg cttcaacata gactctgaaa
    17821 atcggttctg ttcctgacgg acgcatcagc acccagccgt cattcatgta caattttaat
    17881 ccatcggtcc tgtcaatgtt gataatgtcg cgatggtcct tcatttcttc tgctaacaga
    17941 ttcatgagcg gttctttctt ctcattgg
//
LOCUS       DATMXJ010000035         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_16360, whole genome shotgun sequence.
ACCESSION   DATMXJ010000035 DATMXJ010000000
VERSION     DATMXJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16360"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VN377_01040"
     CDS             complement(<1..729)
                     /locus_tag="VN377_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27000.1"
                     /translation="MTKKKTKALCVPLSEAEPVRRTLKERHLLREDVQIVKTETSVYF
                     PVTDIPKDLVNFPSGTKLFEVKTKKPRCYKDLLKLSKKLKEELPTSYDTIGSIILIKL
                     PQSLFQYRKQIGNALIKMHKNIRTVCVVDPVSGELRTRNITIVAGENQTLTTHTEYGL
                     TFQVDVAATYFSPRLASERKRVAGLVKPGEIIVDLFAGVAPFSIMIARYAKPRIIYAI
                     VKNKEAINLAEKNVRQNHVLQTVEV"
     gene            complement(789..877)
                     /locus_tag="VN377_01045"
     tRNA            complement(join(789..824,839..877))
                     /locus_tag="VN377_01045"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Tyr,seq:gta)
     gene            1432..4767
                     /locus_tag="VN377_01050"
     CDS             1432..4767
                     /locus_tag="VN377_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27001.1"
                     /translation="MKGNKIIATMVVLAMLLSTLVVLNQLNVVKQASAQPGVDEWGYS
                     SEELVYGVTYTSGQIEINTSEWAANGTYYLYYPNYWSISPGIATNFSWDGSYVVGADD
                     FPVYVEATQAGVVNSDTLDTLDTGGNSISFARAGMWIFDDNSDHSSFAGYLWINTSTV
                     FSIGSIPDIDFGSEADVNIAVTEDGNNVPCVISVLDPNNNTVYHEYSQGTATLDQSYF
                     DYAGDYTVLAYRDLDKYAVYYYYGDEDDNAYNALYGSNYSGVFPEATDYVYATVGPWD
                     PPEKNATEVTFTVNTGEPTITLTNTTIYWGFDARIDVNVTNSTGVGLDFGSAYPVILK
                     FGSTYVNFSDYITDLGEGDYSIIIPRFDAGDGWADLATAVGDDNVNGTWRVVFGYDAN
                     ADGTYEWNNSATFIVKRSNPPVQLVLSNYQDKKIDFIPAYTGGFVANTITINFAIYGT
                     SITDDIGRAYYGDNSWEDWKNITVTGDILYPVNEDTLEHTGTKGNWTATVTPTKPGGS
                     ITITIDWQGDDNGTASQTIDIINGTHVTPAVDAFTVGSDFNLTVSIKDMDEAALKYAT
                     VYLMWEDEAIALNDTVGDKSAGNGLNGEYTFWITKDDQLATAPQNITIAAEANDFWGY
                     AKVIMDKNHDLSVNVTPTTGYAGNSIEYDIMVGLVSSGHPDEDGLTVELRNETGALVT
                     GDDALAISDNYDITNYAHILSGGTYYLYAYNDTHDSRGHNASIIITNYTVTSSPSVLA
                     WKIDTATNMTFQVTPAANGVLTLYGMSSQAEASSEGDSYPVTIENGTGTIEEVNATTL
                     GNVTYTFEPDGGGERNATGLLRVTTATATPSPATIYNGEATLVTITVTHPATGAPLVD
                     VRVGLDHGMSLNETILAKLPSDQFTDSAGRVQFSITADASGEVTIYLENETDPDNEFV
                     ITATARKPMTITLNPSVDEGKTFTAEAKSNGVLITDATVTFTFDGQTWPTTTGTATIT
                     APTVSTSMAFPITAAAEGYAPTTGSIMVVNIPKLIVAVSGEVKAGQTFTLTIADDTGS
                     PVIGATITFEGKTYTSGAGGVVTITAPSKEGSYPVTATFPGYDSVSGTVTVAKGGGIP
                     GFELLTLIAAIGVAFLLLRRRRK"
BASE COUNT         1436 a         1066 c         1054 g         1292 t
ORIGIN      
        1 gacttcaacg gtctgtagga catggttttg tctgacgttc ttctctgcga ggtttatcgc
       61 ctctttgttt ttaacaatcg catagattat tcttggtttc gcataccgcg caatcataat
      121 agaaaatggt gccacaccag cgaacagatc cacgatgatt tctcctggtt tcaccagacc
      181 agctacgcgt tttctttccg aagctaatcg aggagaaaag tatgttgctg cgacatcgac
      241 ttgaaatgtc aatccatatt ctgtatgggt cgtcagcgtt tggttctctc cggcgacaat
      301 cgtaatattt cgggtacgga gctctcctga aacagggtca actacacaga ccgttcgtat
      361 gtttttgtgc atctttatga gagcattgcc aatttgttta cgatactgaa aaagcgattg
      421 aggcaatttt atcaggataa ttgatcctat cgtatcatac gatgttggca gttcttcctt
      481 tagttttttt gaaagtttca gaaggtcttt gtaacaacga ggtttttttg ttttcacctc
      541 gaataatttg gtgccactcg ggaagttcac cagatcctta ggtatatcag tgacaggaaa
      601 ataaacagat gtttccgtct ttactatctg aacatcttct cttaaaagat gtcgttcttt
      661 gagagttctt cgtactggtt cagcttcact caaaggaaca cagagagctt tcgttttctt
      721 ttttgtcatt gcacctgatg tgtatgacat tcttcaataa atagtacggt atcttgagaa
      781 gaaaagagtc ccgcctcccg gatttgaacc ggggacctgc cggtaacggc catagagtct
      841 acagccggcc gctccagcca gactgagcta aggcggggaa tctgttctag cccacataat
      901 aataggtata tttattagtt aaggtttctt tccgaagctt gttaggtatg aggtttggtg
      961 taatcgtctg tccgcagtgc aggcaagtga aagggttgga gttatcatct aagacaacaa
     1021 aatgtatacg atgtggaaaa acgcttcaaa tgaaaaaaat aacaatcttt tatgaaaccg
     1081 attctcaaga gaagctacgg cgagcgattg gtcttttgaa tgccaagttg gatgaaaata
     1141 aaagtgcgaa caagaaattt ttattcgata cacacacatc gaaatgaagt atgatttatg
     1201 tcattaatct aaattttata ccaatagttg tttataagat aaggaaggaa aatcgtaatg
     1261 tatataaaca aggtagatat ttacctcacc tgaggtccgg tgaggacacg gatataagtt
     1321 acctacatct acactagtga gggacttata tagtggttca cggtgtgaac caggtgtttt
     1381 acctgaccat tatgaagcaa tatgcaacat gaatttagga ggacacaaaa aatgaaagga
     1441 aacaaaataa tcgctaccat ggtagtactg gcaatgttac tatcaacgct ggtagtttta
     1501 aatcaattaa atgttgtaaa acaagcgagt gcacaacccg gtgttgatga gtggggttat
     1561 tcatcagagg agcttgtcta tggagtcaca tatacaagtg gacagattga gatcaacact
     1621 tcggaatggg ctgcaaatgg cacctattac ctgtattacc cgaattattg gtctatttct
     1681 ccaggtatcg caacgaattt ttcatgggat ggatcatacg tagtaggagc ggacgatttt
     1741 ccagtatatg ttgaagcgac gcaagcaggt gtagtaaata gtgacaccct agatacactg
     1801 gatacaggag gaaactcgat ttctttcgcc agagcaggta tgtggatttt cgatgataat
     1861 tcagatcata gttcattcgc tggatatttg tggattaaca cttcaaccgt ttttagtata
     1921 ggctctatcc cagatattga ctttgggtca gaagcagatg tcaatatagc tgtaacggaa
     1981 gacggtaaca atgtaccctg tgtgatatca gtcctcgatc caaataataa taccgtgtat
     2041 cacgagtact cccaaggaac cgcaacatta gaccaaagtt acttcgatta tgctggtgat
     2101 tacaccgtat tagcctatcg agatcttgac aaatatgcag tatactatta ttatggcgat
     2161 gaagacgaca atgcttataa cgcattatac ggaagcaatt acagtggcgt tttccctgaa
     2221 gccactgact acgtttatgc aaccgttggc ccttgggatc ccccagaaaa aaatgcaact
     2281 gaagttacat ttacagtaaa cacaggagaa ccaacgatca cgttaacaaa cacgacaatc
     2341 tactggggat ttgacgcacg gattgacgtc aacgtaacaa acagcacagg agtaggtctc
     2401 gactttgggt cggcttatcc agttatacta aagttcggat caacatacgt caatttcagt
     2461 gattacatca ccgatctcgg tgaaggcgat tattctatta tcatcccacg attcgatgca
     2521 ggtgatggct gggcagatct tgctacagca gttggtgacg ataacgtcaa tggaacctgg
     2581 cgagtcgtgt tcggatatga tgcaaatgcc gatggtacct acgaatggaa caactctgca
     2641 acctttatcg tcaaaagatc aaatccacca gtgcagttgg ttctttcaaa ttaccaggat
     2701 aagaaaatcg actttatccc tgcgtatact ggaggattcg ttgccaatac cattactata
     2761 aattttgcca tctacggcac cagtattact gacgacattg ggcgagcata ctacggagat
     2821 aattcatggg aggattggaa gaatattacc gtaacaggtg atatcttata tcctgtcaat
     2881 gaggacacac ttgaacatac tggaacgaaa ggaaactgga ctgcaactgt tacaccaaca
     2941 aaacctggag gatcaatcac aataacgatt gattggcagg gagatgataa cggaacggca
     3001 agtcaaacta ttgatatcat caacggaact catgtcaccc ccgcggtcga tgcattcacg
     3061 gttggatctg attttaacct caccgtttcc atcaaagata tggatgaggc tgccttgaaa
     3121 tacgcgactg tttatttaat gtgggaagat gaagccattg ctttaaatga tactgtaggt
     3181 gacaaatctg cagggaacgg tctcaatgga gaatatacct tctggatcac caaagatgat
     3241 caactggcga ccgctccaca aaacattacc atagcggcag aagcaaatga tttctgggga
     3301 tacgcaaagg tcattatgga caaaaaccat gacctctcag tgaacgtcac tcctaccacg
     3361 ggatatgccg gtaacagtat tgaatacgat atcatggtcg gtcttgtcag tagtggacat
     3421 cctgatgaag atggtttaac agtcgaatta cgtaacgaga ctggagcact tgtgactgga
     3481 gatgatgcat tagctattag tgataattat gatatcacaa attatgcgca tatcctctct
     3541 ggaggaacct attacctcta cgcctacaac gatactcatg acagccgcgg acataacgcg
     3601 tccatcatta tcaccaacta tacggtcaca tcttcaccat cggtccttgc atggaagatt
     3661 gataccgcga caaatatgac cttccaggtg acaccagcag caaacggtgt gttaacactc
     3721 tatgggatgt ccagtcaggc cgaagcatct agtgagggag actcgtatcc agtaaccata
     3781 gaaaacggaa ctggtaccat cgaagaagtc aatgcaacca cccttgggaa tgtcacctac
     3841 acattcgaac ccgatggcgg tggagaacgg aatgccacag gtttactccg cgtgacaacc
     3901 gcgactgcaa caccaagccc agcaaccatc tacaatggtg aagcaacctt agtaacaatt
     3961 actgtcaccc atcctgcgac aggggctccc ttggtagacg ttcgtgtcgg gctcgatcat
     4021 ggaatgtccc tcaatgagac aattcttgcc aaattaccct cggatcaatt cactgactca
     4081 gctggacgag tacaattctc aatcactgca gacgcaagtg gtgaagtaac tatctacctc
     4141 gaaaatgaaa ccgatcctga caatgaattc gttatcacag caacagctcg aaaaccaatg
     4201 accatcaccc tcaatccatc tgttgacgaa ggaaaaacct tcaccgctga ggcaaaatcc
     4261 aacggtgtcc ttatcaccga tgccactgta acgttcacct ttgatggaca gacctggccg
     4321 accacaaccg gtacggcaac catcaccgct cccactgtat cgacctcaat ggccttccca
     4381 atcacagcag ccgctgaagg atacgcccct acgaccggtt ccattatggt cgttaacatc
     4441 ccgaaactca tcgttgccgt cagtggagaa gtaaaagcag gacagacatt caccttaacc
     4501 atcgctgacg ataccggtag ccccgtcatt ggtgcaacaa tcacctttga gggaaaaacc
     4561 tacacgagcg gcgctggagg agttgtcacg atcactgcac catcaaaaga aggcagttat
     4621 ccagtgacag cgaccttccc aggatatgac tcggtttcgg gtactgtgac cgtggcaaaa
     4681 ggtggcggga tcccaggatt cgagttactg acactcatcg cagcaatcgg agtagcgttc
     4741 ctgctcctca gacgcagacg aaagtaaaca cttaaggggt ttaaccccct tttttctcct
     4801 ttttttaatg tttttttcaa aatcctttta acctccttgt ttttatac
//
LOCUS       DATMXJ010000036         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_42423, whole genome shotgun sequence.
ACCESSION   DATMXJ010000036 DATMXJ010000000
VERSION     DATMXJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42423"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..519)
                     /locus_tag="VN377_01055"
     CDS             complement(46..519)
                     /locus_tag="VN377_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27002.1"
                     /translation="MKFTDTIKQHMKREIVYTTEDERLADVIFKMANAGTDIAVVKFK
                     DDIVGVITETDIYFALVKEVFSEVSLVIEDAHVIDILRGPHTKEVMASCDPLGWHPCI
                     DTFEDDTMENAIRIMQRSGLHHLLVLDKKNKLVGTLSSHDIIKSFSRREVQIKKK"
     gene            complement(566..781)
                     /locus_tag="VN377_01060"
     CDS             complement(566..781)
                     /locus_tag="VN377_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWR27003.1"
                     /translation="MIVKIEQDECIECGACEQTCSKLFVVESGQKASIVEKYRKNISS
                     IGEVPDALVSCANDAVIACPVQVISIE"
     gene            complement(799..1653)
                     /locus_tag="VN377_01065"
     CDS             complement(799..1653)
                     /locus_tag="VN377_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWR27004.1"
                     /translation="MTDDLTTPVENTWCPGCGNFGILNAFQKAIQKLEVQGIDREHLV
                     MSAGIGCHGKIFDYVPLSGFYSLHGRSMATVQGIKLGNPNLKVLTFAGDGDAFGEGIS
                     HMIFAAKRNADITVIVHNNGTYALTTGQFSPTSPKNFKGPSLPSGNVEEPLNPLSLML
                     EAGASFVARGYAGRIDHLAELIVEAVLHEGFSYIDVLQPSVVFSNTYKEYNSLVEMIE
                     KPAVRLEDAVQLAKRFDKIPIGVFYKVSKPVYHRELYGEWNPVMNRLPHAERMRRVAM
                     YLSDRTQE"
     gene            complement(1654..>3102)
                     /locus_tag="VN377_01070"
     CDS             complement(1654..>3102)
                     /locus_tag="VN377_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWR27005.1"
                     /translation="AILLDQRSYDIHQNHLAEKSMLIYDSTTVKQGQGIGVPISSLAK
                     KYANSELIVGIAGIAALVAAVGADRPRLDSIIQKEYHRNVEENLKFAHEIYDNVVEKI
                     PQKFVLQDGLETHPILTGAETIGLGAYTAGLDLYFAYPMTPSTPILHFLASQGKKLGF
                     VTMQPENEIAVANMAIGAAFTGARSMVGTSGGGFCLMQEAFSLAGMTETPVVFFLGQR
                     PGPSTGVPTYTEQGELLFSLYPGQGEFPRIVASPGSIEEAFYLSAELLDLAWRFQTPA
                     ILLSEKHLTESSMNVDLKMEKIQEAVPDLHTKGSYQRYRQTDTGVSPLLFPPSDMMIK
                     WNSYEHDEQGFTTEEADVIARMHKKRLTKLESLIRYLQENVQTVNEFGSGNPCIFTFG
                     STTMSVREAVEHADLSCTVVQPIYLQPFPSWNLMRYVGRKAIVVEQNSTGQLEQLLRE
                     KIGITPVSSIRQFDGRPFDPIGLAEQIRKVIG"
BASE COUNT          856 a          791 c          558 g          897 t
ORIGIN      
        1 gtataaaaaa cacctctact acaaaaacgt ttaatttaaa aataattatt ttttctttat
       61 ttgaacttct cttctgctga aactttttat gatgtcatga gatgatagag ttcctactaa
      121 tttgtttttc ttatcaagaa ctaacaaatg atgcagcccg ctccgttgca tgattcggat
      181 agcgttctcc atagtatcat cctcaaacgt atcaatacaa ggatgccaac ctagcggatc
      241 acatgaggcc attacctctt ttgtatgagg ccccctcagg atatcaatga catgtgcatc
      301 ctcaatcacc aaagaaactt ctgaaaatac ttctttcaca agtgcaaaat aaatatctgt
      361 ctcggtaatc acgcccacaa tatcgtcttt gaattttacg accgcgatat cggttccagc
      421 gttcgccatc ttaaatatca cgtctgctaa tcgttcatcc tctgtcgtat acactatttc
      481 tcttttcatg tgctgtttaa tggtgtcagt aaatttcatg gtttcatctc catcttttca
      541 acgtttcaat tatcttattt gcttctcatt cgatgctaat cacctgaacc ggacatgcta
      601 taacagcatc atttgcgcag ctcacaagtg catcaggaac ttcacctata cttgatatgt
      661 ttttccggta tttctctaca atactcgcct tctggccact ttcaacgaca aataattttg
      721 aacaggtctg ctcacatgcg ccacattcaa tacattcgtc ttgttctatt ttcactatca
      781 tttcttatga cctccgcttt attcttgcgt tcgatccgag aggtacattg caacacgtcg
      841 catccgctca gcatgtggca atcggttcat cacaggattc cattccccgt ataattcccg
      901 atgatagacc ggctttgaaa ccttatagaa tacaccgatc ggtatcttat caaaacgttt
      961 cgcaagttgc acggcgtctt ccagccgcac tgctggtttt tcaatcatct caaccagcga
     1021 attatattcc ttatacgtat tactgaatac gacactgggt tgcagcacat cgatgtacga
     1081 aaaaccttca tgcaataccg cttcaacaat tagctcagca agatggtcaa tacgcccggc
     1141 gtaccctcgt gcaacaaatg atgcgcctgc ctctagcatc aatgacaacg ggttcaacgg
     1201 ctcctcaacg ttcccacttg gcaatgatgg acccttaaag tttttcgggc ttgtaggaga
     1261 aaactgcccg gttgtcaacg catacgtccc attattatgg actatcacgg tgatatctgc
     1321 attccgtttc gctgcaaaaa tcatatggga aatcccttca ccaaatgcgt ctccatcacc
     1381 agcaaacgtc aatactttta aattcggatt ccccagttta ataccttgta ccgtcgccat
     1441 ggatcgacca tgaagcgaat aaaatccgct caacggcaca taatcaaaaa tcttcccatg
     1501 acatccaata ccagcagaca tcaccaaatg ttcacgatcg ataccctgga cctctaactt
     1561 ctgtatcgct ttctgaaatg cattcaagat accaaaattc ccacacccgg gacaccaggt
     1621 attttcaaca ggggtcgtta aatcatcagt catttacccg atcaccttcc gtatttgttc
     1681 agcaagacct atcggatcga acggccggcc atcgaattgt cgaatcgacg agaccggggt
     1741 gatcccaatt ttttcacgca acaactgttc taactgcccg gtactattct gctcaaccac
     1801 gatcgccttg cgtccaacgt atctcatgag attccatgaa ggaaatggct gcaggtaaat
     1861 aggctgaacc acagtacatg aaagatcagc atgttccacc gcttctcgaa cactcatggt
     1921 tgtcgaccca aacgtaaaaa tacagggatt accagatccg aactcgttta ctgtctgaac
     1981 gttttcctgg agatatcgaa tcagactttc tagctttgtc aaacgcttct tatgcattcg
     2041 cgcgatcaca tcagcctctt ccgtcgtaaa cccttgttca tcatgctcat agctattcca
     2101 tttaatcatc atatcagacg ggggaaataa gaggggtgat acaccggtat ccgtctgacg
     2161 ataccgctga taacttccct tcgtatgcaa atcaggtaca gcttcttgta ttttctccat
     2221 tttaagatcc acattcatac tactttcagt gagatgtttc tcggagagta agattgcagg
     2281 tgtctgaaat cgccatgcga gatcgagcag ttctgcggat aaataaaatg cttcttcgat
     2341 actccctggt gaggctacga ttcttgggaa ttctccttgt ccagggtaca aagaaaagag
     2401 aagttcacct tgttctgtat aagtaggtac tcctgtacta ggacctggac gttgaccaag
     2461 aaagaacacg acaggtgtct ctgtcattcc agcaagggaa aaagcctcct gcatgagaca
     2521 gaaaccccca ccgctcgtcc caaccatgct tcgagcgcca gtaaacgcag caccaatcgc
     2581 catgttcgca accgcaatct cattttccgg ttgcatcgtt acgaacccta acttttttcc
     2641 ttgtgatgca agaaaatgca aaatcggcgt tgatggtgtc atcggatagg caaaataaag
     2701 gtcaaggcca gcagtataag cacccaaacc aatagtttca gcacccgtca aaatcggatg
     2761 ggtttccaaa ccatcttgta acacgaactt ttgcgggatt ttttcaacaa cattatcata
     2821 gatctcgtgg gcaaatttga ggttctcttc aacattccga tgatactctt tttggataat
     2881 actatcaagt ctcggacgat cagcacccac tgctgctaca agtgctgcaa tacctgcgat
     2941 cccgacgatt agctcagaat tagcatattt tttcgcaaga gaagaaatcg gcacccctat
     3001 accttgacct tgtttcactg tcgtactatc ataaatcagc atgctctttt ctgcaagatg
     3061 gttctgatgt atgtcatagc tccgttgatc aaggagaata gc
//
LOCUS       DATMXJ010000037         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_42448, whole genome shotgun sequence.
ACCESSION   DATMXJ010000037 DATMXJ010000000
VERSION     DATMXJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42448"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VN377_01075"
     CDS             complement(<1..1108)
                     /locus_tag="VN377_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HWR27006.1"
                     /translation="MSFPKPPEVNIGMIGHVDHGKTTLTQRLTGKWTDEHSEELKRGI
                     SIRLGYADAAFYKCSTCDDPACYSTQKVCPNCKGKTELLRVVSFVDAPGHETLMATML
                     SGAAIMDGAVLVIAANEVCPQPQTREHLMALEIIGAKRIIIVQNKIDLVSEEDALTNY
                     EQIVKFVKGTCAEGAPIIPVSAHHDINIDVLIKTMEEYIPTPKRDMEKPPLMYVARSF
                     DVNKPGTKLSELKGGVVGGSLLQGTLKKGEKIEIAPGRKREVEGKQVLEPIFTTAHSL
                     ITGGTVTNIALPGGLIAIGTELDPTMVKSDSLAGKIVGRPGQLPPVLDTIDLSMCLLK
                     RAVGTIDEKNVEPLKTNEPLMLTVGTATTVGIIIE"
     gene            complement(1108..1545)
                     /locus_tag="VN377_01080"
     CDS             complement(1108..1545)
                     /locus_tag="VN377_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HWR27007.1"
                     /translation="MVEFKVVVNDIQTGKSYNIPVSGHHANSLIGKKINDEVDGIFVS
                     LPGYKLQITGGTDKDGFPMRHDVPGSTRRRLLLSRGLGFKPTERGKRKKKSVRGNTIN
                     QDIVQVNMKVMKPGAKPIDEIIKGEGKTETKEATASKEKKEEQ"
     gene            1781..2392
                     /locus_tag="VN377_01085"
     CDS             1781..2392
                     /locus_tag="VN377_01085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HWR27008.1"
                     /translation="MYALAKIHDTIRVPPERFGEDINEAINDIVQKTFEGIIRRNHGL
                     IVVVDNITPLGDGIVIHGDGGMYQKVEFEALTFNPLLQELVDGIVCEIVEFGAFCHIG
                     PLDALLHMSQIMNDYVDVDAESGRIQGKETKRILKVGDPLRARIVAVSLNELSARESK
                     IGLTMRQPALGTHEWLTEPKDEKRPEKKREDRKEKREERGEKK"
     gene            2389..2574
                     /gene="spt4"
                     /locus_tag="VN377_01090"
     CDS             2389..2574
                     /gene="spt4"
                     /locus_tag="VN377_01090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HWR27009.1"
                     /translation="MKNLNACKTCHFLTEKDRCPNCSDQTVSRWRGYVIVRDPEHSQV
                     AKKMNITKPGKYALKVS"
     gene            2577..>3008
                     /locus_tag="VN377_01095"
     CDS             2577..>3008
                     /locus_tag="VN377_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HWR27010.1"
                     /translation="MYVLPEDLKDTLKGYIGQLVDEPDLIKLLKKEKSVVTIGDKVTY
                     TVLKHGFTPLLCIVDFILERKPYPSEMKTLIQGYGRVHLKIKNPPGTITDELWDAIQT
                     VYKNPQKSPVCIEVDGEEDLASLAAIYLAPQGVTVIYGLPNR"
BASE COUNT          800 a          705 c          660 g          843 t
ORIGIN      
        1 gttcaataat gataccaacc gtcgttgctg tcccaaccgt cagcatcaat ggttcattcg
       61 ttttcaatgg ttctacgttt ttctcatcaa tggttccaac agcacgcttc aataaacaca
      121 tggacagatc aatcgtgtca agcactggtg gaagttgtcc gggtcgtcca acgatttttc
      181 ctgccaagga gtctgatttg accatcgttg gatcaagctc tgtgcctatt gcgatcaacc
      241 cgccgggtaa cgcgatatta gtaactgtgc cgccagtgat aagtgaatgt gcggttgtga
      301 agattggctc aaggacttgt ttcccttcga cctctcgttt tctcccaggt gcgatctcga
      361 ttttctcacc tttcttaagg gtcccctgta gtagggaacc tccgacgaca ccgcctttta
      421 attcactcag tttcgttcca ggtttattga catcgaaact ccgagcaaca tacattagtg
      481 gaggtttctc catatccctc ttcggcgttg ggatgtactc ctccatggtt ttgattagca
      541 catcgatgtt gatgtcatga tgagcactga ccgggatgat tggtgctcct tccgcgcagg
      601 ttcctttgac aaacttcacg atctgctcat aattcgtaag agcgtcttcc tcagaaacaa
      661 gatcaatttt attctgaaca atgataatcc ttttcgcacc aatgatctct aaagccataa
      721 gatgttctcg cgtctgcggc tgaggacaga cctcatttgc tgcgatcacc agaacagcac
      781 catccataat ggccgcacct gaaagcatgg ttgccatcag cgtctcatga cctggtgcat
      841 caacaaaaga aacaaccctg agcaactcgg ttttcccttt gcagttcgga cagaccttct
      901 gcgtcgaata acacgcagga tcatcacagg tggaacattt ataaaatgca gcatccgcgt
      961 agccaagacg aatggagata cctcgtttta actcctcaga atgctcatca gtccacttcc
     1021 cagtcagtcg ctgagtgagc gttgttttcc catggtcaac atgcccgatc attccgatat
     1081 tgacctcggg aggtttcgga aaactcatta ttgttcctct ttcttctcct tcgatgctgt
     1141 tgcttccttt gtttctgtct tcccttcacc ttttatgatt tcatcaattg gtttggcacc
     1201 aggtttcatg accttcatat tcacctggac gatatcttga tttatcgtgt tccctcggac
     1261 cgattttttc ttccgtttcc cacgctctgt tggtttaaaa ccaaggcctc tgcttaacag
     1321 caaacgtctg cgggtagaac ccggaacatc atgtctcatc gggaacccat ctttatcggt
     1381 ccccccggta atctgaagtt tatatcctgg aagcgagacg aagatcccat cgacctcgtc
     1441 gttgattttt tttccgatca acgagttggc atgatgtcca ctgaccggga tgttatatga
     1501 ttttccagtt tgtatatcgt tgacaacgac tttgaattca accatgattc tttctcctta
     1561 taattcttct tctattggag taactttgta acgaagtgtt acaagaaggg gaatagcgag
     1621 tattttataa atcttctcct taacaaaaaa ccaccttgat tttccttggt cttatgaaga
     1681 gatttcaccc tattttttac catcttctga cactcaaaaa gaatttaagg gataagtcta
     1741 ttccagttcg aactgtaatt caggattaaa acgtgagaga atgtacgcct tagcaaaaat
     1801 acatgataca attcgagttc ctcctgaacg atttggagaa gatatcaacg aagcgattaa
     1861 tgatatcgtc caaaaaacat ttgaaggaat tatccgacga aaccatggat taatcgtcgt
     1921 tgtcgataac atcactccat taggagatgg tatcgtgatt catggtgatg gagggatgta
     1981 ccagaaagta gaattcgaag cccttacctt taacccgctc ttacaggaac tggtcgatgg
     2041 gatcgtctgt gagattgtcg agtttggagc gttttgccat atcggacctc tcgatgcctt
     2101 gttacatatg agtcagatca tgaacgatta tgtggacgtc gatgcggaga gcggacggat
     2161 ccaaggcaaa gaaaccaagc ggattctgaa agtcggtgac ccgctccgtg cacgtatcgt
     2221 tgcggtcagt ctcaacgagt tatctgcacg agaaagtaaa atcggtctta ccatgcggca
     2281 acccgcgctt ggaacccatg aatggctcac cgaaccaaaa gatgagaaac gtccggagaa
     2341 aaaacgtgaa gatcgcaagg agaaacgaga ggaacgaggt gagaagaaat gaagaatcta
     2401 aacgcctgta aaacctgcca ttttctcaca gagaaagacc gttgcccgaa ttgttcagat
     2461 cagaccgtca gtcgatggcg tgggtatgtg attgtccgtg accctgagca ttcccaggtt
     2521 gctaagaaaa tgaacatcac aaaaccaggg aaatatgcct tgaaggtcag ttaaggatgt
     2581 atgtgctacc tgaggactta aaggacacac tgaaagggta tatcggtcag ctggttgatg
     2641 aaccagacct tataaaactg ctaaaaaaag aaaaatccgt tgtcactatt ggtgataagg
     2701 ttacctatac tgttctgaaa catggtttta ctcctcttct ctgtatcgtg gattttatcc
     2761 tggagcgcaa accgtatccc tcggagatga aaacgctcat tcaggggtat gggcgggtcc
     2821 atctcaagat aaaaaaccct cctggaacca ttaccgatga actctgggac gcgattcaga
     2881 cggtgtataa aaacccccag aagagcccgg tgtgtatcga ggtggacggc gaggaagacc
     2941 ttgcctcatt ggccgctatc taccttgcac cccaaggcgt aactgtaata tatggattgc
     3001 cgaatagg
//
LOCUS       DATMXJ010000038         8675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_32071, whole genome shotgun sequence.
ACCESSION   DATMXJ010000038 DATMXJ010000000
VERSION     DATMXJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32071"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VN377_01100"
     CDS             <1..242
                     /locus_tag="VN377_01100"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWR27011.1"
                     /translation="FVEVYVRCPLKICMKRDVKGMYRKALEGKIKHFTGVDDPYEEPE
                     NPDLIVDTDIESVEESVGKVLQKIEELGYLEEKKD"
     gene            complement(276..1484)
                     /locus_tag="VN377_01105"
     CDS             complement(276..1484)
                     /locus_tag="VN377_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWR27012.1"
                     /translation="MLYDVAIVGAGPAGSTAAKLLSEKGIRTLLLDKASFPRDKPCGG
                     GLQMRVLHRFKYLEENNLIDSYSTTFQIHTSTMAHHLDFHHPKPLQAMVLRKHFDEGL
                     VNLAIRNGAVLQCGNTVQDMINEKDTMRLILSDGTTFESRLIIGADGMWSTIAKKIGM
                     KQECDHIGVSVYAEYPMCQETIHKVYGSDQGVHIHLQPNGIAGYGWVFPKKEHVNIGV
                     VEFRQAINPTRGKKNLQTSYAHYLQSLKQQKLIPDNLSGATTRGGAFPTCPMKRLTAD
                     RVLLCGDAGGLTNPMTGEGIFYAMCSGEMAAKTAIKALENNTTDTQSLRDYHRRWNQE
                     FRGDFTFVNQLSKRWGRNIDRLIEIACRDKKLIDVICQAIPTPRGVQQEKWKIIRRFV
                     FAYCKNRLKQ"
     gene            complement(1605..2126)
                     /locus_tag="VN377_01110"
     CDS             complement(1605..2126)
                     /locus_tag="VN377_01110"
                     /inference="COORDINATES: protein motif:HMM:NF021561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HWR27013.1"
                     /translation="MSATPLPPPKYFIHRACELGAIEATVISPQHVYTAEWVRRKCQY
                     GCDGYGEHLTCPPYSPTPQETRRLLDEYTTALLIHCPSRGVEVLQIIYTLEREAFLAG
                     YYKAFGMSAGPCSLCERCNVQKTCCHTEQARPSMEACGIDVYQTARTAGYPIEVVRDH
                     TCPENYYALLLLE"
     gene            complement(2225..3355)
                     /locus_tag="VN377_01115"
     CDS             complement(2225..3355)
                     /locus_tag="VN377_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR27014.1"
                     /translation="MILEFLIVLGIIGILGECYIISLWLIAYRRTKKRIFQPYFPYVS
                     VIVPCKGTGHGFLENIQGFLAQEYQKYEIIFVVDSTDDPAYTTLQQLIENKPYAHLVL
                     TDSVSGCSGKIAALLTGLKSTEKTEVLVFADSDIKPDTHWLQNIVMPLQDEAIGVTTG
                     YRWYFPTNWKTLLISAWNMASIVFMFYPSYTFAWGGSMAIRKKVFDTLDIKEKWKTAF
                     SDDLILTISVKKAGYKIYLQPKCIMESPPETRITSFIRWGTRQYTWVRWYYPVFWIGS
                     FVGFIGAQVVIFLGLLLLLFGYYLPGFLLCSMLLFEILYGWLGITILPKTMIYPKERY
                     PSKIGYALLTPIVFLLLAQNAFSSAITREIQWAGRTYRKPKN"
     gene            3485..4462
                     /locus_tag="VN377_01120"
     CDS             3485..4462
                     /locus_tag="VN377_01120"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWR27015.1"
                     /translation="MINHTDTRKDRQYLKGLENIDFRPVFILGVHRSGTSILYKMLTA
                     TGCFNPVTAYHLITYDELLSNYHEQKEHGAKQQLTESFLKNGLKDRGIDRLKITADFA
                     EEYGFLLGNQTLQMRITKKNVALFIELCKKIQFIAGNKKPILLKNPYDFPNFLYLKEV
                     FPNARFVFIHRHPLKTISSTLNAIRTIVKERNPYTTRLSKVYDKFYSNPLLLQPLRFI
                     VHTMAECCVVVLTRITKKAVEYYVKNIEKLPKDDYISITYEDFCVHPQETLQNIMDKL
                     SLTMIQQIDASSLMNPRKVDIDSSVEKLRIYIYKSMEEYFDLFHYTIDR"
     gene            complement(4554..5777)
                     /locus_tag="VN377_01125"
     CDS             complement(4554..5777)
                     /locus_tag="VN377_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HWR27016.1"
                     /translation="MKKIIVIILVAATILNIVAGAFLFLDIQQMDIPETTLALEIITI
                     TTDEAVLQTTLSINNHNSFPVILQNITMITTDENGETINHLTMPGGETKAHENRSFTT
                     TANIRFDGSLPTHLTSRLTGTFGTIFFGIIKKTFPLKFTIETSLNNILEQFTLPHIHL
                     AANFSDITQEGINFTGVIEITNPNTIDISVENLSADITTETGIPVGTVTIQGETIPAK
                     TTKQLTGSGWILLKALNAVSLRMTLTGDIIISIAGIRKPMNISLEADIIPPYLGQLLS
                     DLPTEASLTGVYTYTLKDGLHDEVTFTIKNPNKLTLLANDITVQIYRVDGTKTRMISN
                     GTLPDGFIAPQSTTVLQGDMFIPLTQLWPRRGERFIPDQLQVILRSNVTIQGFNQTIW
                     IGVIGYQDFPFHRFS"
     gene            complement(5943..6356)
                     /locus_tag="VN377_01130"
     CDS             complement(5943..6356)
                     /locus_tag="VN377_01130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27017.1"
                     /translation="MTLKDYDSVIALWAEGNIPYRPQGRDSRKNITIQLQQPNCYFLV
                     AETDKRIIGAIVGTHDGRKGWINRLVIAPSYQKKGIAKRLVEEVEHHFSSMGIDIIAC
                     LIEDWNTTSMQVFEKLGYTKHTDILYYSKRKSMKT"
     gene            complement(6555..7544)
                     /locus_tag="VN377_01135"
     CDS             complement(6555..7544)
                     /locus_tag="VN377_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27018.1"
                     /translation="MNLKTTVFEYAKLSSAHFIFLTAIIPLTGAIAMGEKDLFLLTVL
                     FLIGLSAHIVGFALNHYVDVEVDRLISSTSKRPLPSGSISKRNALAYILLVLLLGCLL
                     TLYFYGVILLLLYLFGILLAGFYDLYSKRICGMDFFLAVSVMVGVVFGAATVSFQFSS
                     LVIILCILAFLQTLNLNLVAGGIKDADHDYLMQSQHLSTRLGVRVEQGTLRIPASFQL
                     IAYLLGLLYAFCVFLPIFFGVIHLDISLILPLILINVLFFIVTYKMVHLRSFYRQEVR
                     KYVVLQYMINWLNIPILLMSVAPLAGFLVLYPIIGLVVSNVILYRTIVRPQVM"
     gene            complement(7980..8318)
                     /locus_tag="VN377_01140"
     CDS             complement(7980..8318)
                     /locus_tag="VN377_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27019.1"
                     /translation="MKRLFIGIGILAILSISYLSGCLDEKSKFIGTWETQDGSTSMTF
                     DTNNKVTISGSGPLAIVSLTGLFAYSLANQKITFSSGDTGITLNYSFPSSNQLILSND
                     QGASLVLTKK"
     gene            complement(8420..>8675)
                     /locus_tag="VN377_01145"
     CDS             complement(8420..>8675)
                     /locus_tag="VN377_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27020.1"
                     /translation="DQNAESVNWSISVTGGILKRIDLRSTGTINSLSTQSESTVITDR
                     IPLGLGRLEITVTAQTSGGESVTQTAKGFKFLFFVILVRM"
BASE COUNT         2457 a         1736 c         1960 g         2522 t
ORIGIN      
        1 atttcgttga agtgtatgtt cgttgtcctt tgaagatctg catgaaacgt gacgtgaaag
       61 gcatgtatcg aaaggctctt gaggggaaaa tcaagcattt caccggggtt gacgatccat
      121 atgaagagcc ggagaatcct gaccttatcg ttgataccga tatcgaatct gtggaagaaa
      181 gtgtcggtaa ggttttgcag aaaatcgagg aactcgggta tctagaagag aaaaaagatt
      241 agaaagagcg ggaaatcgat tttttaattg ctgtttcatt gcttgagtct gttcttacaa
      301 taggcgaaga caaagcggcg gataattttc catttctctt gttgtactcc tctgggtgtt
      361 gggatcgcct ggcagataac atcgatgagt tttttgtctc tgcatgcaat ttcgattaac
      421 cggtcaatat ttcgccccca gcgtttcgat aattgattca caaaagtaaa atccccgcgg
      481 aattcttggt tccatctgcg gtggtagtct ctgagggatt gagtatctgt tgtgttattt
      541 tcgagtgctt tgattgctgt ttttgcagcc atctcaccgg agcacatcgc ataaaagatt
      601 ccctcaccag tcattgggtt tgtaagtccg cctgcatcac cgcaaagtaa tactcgatcc
      661 gcggttaatc gtttcattgg acatgtcggg aatgctcctc cacgtgttgt tgcaccgcta
      721 agattgtcgg ggataagttt ttgttgtttc aaggactgaa gatagtgtgc atagctcgtc
      781 tgtaggttct tttttcctct tgtagggtta atggcttgtc gaaattcgac gactccgatg
      841 ttgacgtgtt cttttttggg gaacacccag ccgtatccag caattccgtt tggttgaaga
      901 tgtatatgaa ctccttgatc ggaaccatat accttatgaa tggtttcttg acacatagga
      961 tattcagcat acacgctgac tccgatatgg tcacattcct gtttcatacc aatttttttt
     1021 gcaatcgtgc tccacattcc atccgcaccg atgatcaatc gtgattcaaa cgtcgttcca
     1081 tctgaaagaa tgaggcgcat ggtgtctttc tcgttgatca tgtcttgtac agtgtttccg
     1141 cattgcagga ccgccccgtt tcgtatcgca agattgacta ggccttcatc aaagtgtttt
     1201 cgaaggacca ttgcctggag gggtttgggg tggtgaaagt ctaaatgatg tgccatggtc
     1261 gatgtgtgga tttggaacgt tgtcgagtat gaatcaatca aattgttttc ctcaaggtat
     1321 ttgaatcgat ggaggactcg catctgaagc ccacctccac agggcttgtc tcggggaaat
     1381 gatgctttgt cgagaagaag ggtgcgaatg cctttttcgg agaggagttt tgctgctgtg
     1441 gaacctgctg gaccagcccc gacgatagcg acatcataga gcatagaaca atcattcctc
     1501 cggtttcact ttttctaatg ctcttatgat atataaattt tacagaaaag gactatataa
     1561 tgccattttt attaataaat aggagagtat cagatgattt tctattattc aagtaaaagt
     1621 aaggcgtaat agttttcagg gcaggtatgg tctcggacga cctcgattgg atatccagcg
     1681 gtccttgctg tttgatagac atcgatgcca caggcttcca ttgagggtcg tgcctgttcc
     1741 gtgtgacaac atgttttctg cacgttgcat ctttcacaaa gggagcaggg accagcgctc
     1801 atcccgaacg ccttgtagta tcctgcgagg aacgcctcac gttcaagggt gtaaatgatt
     1861 tggagcactt caactcctct tgatggacaa tggatcaaaa gagcggtggt gtactcatcc
     1921 agcaaccgtc gtgtctcctg tggtgttggt gaatagggag gacaggtgag atgctcgcca
     1981 tagccatcac atccgtattg gcattttcgt cgaacccatt cagcggtata gacgtgctgt
     2041 ggggagatca ccgttgcttc tattgctcct aattcacagg ctctgtgtat gaagtatttt
     2101 ggtggtggaa ggggtgttgc actcatcaca tcagaaataa ttacttgcga tttaaattaa
     2161 tctatataca gtgcatggga agggatttga acccgtgtat gatggataat cgatttatta
     2221 aatcctagtt ctttggtttt cgataggttc gtcccgccca ttgaatctct cgtgttattg
     2281 ctgacgaaaa agcattttgt gctaataaaa gaaagacgat cggagtcagg agggcatatc
     2341 ctatctttga tggatagcgt tcttttgggt aaatcatggt tttcgggagg atagtgatcc
     2401 ctaaccagcc atacaatatt tcgaacagaa gcatcgaaca taataaaaaa ccggggaggt
     2461 agtatccgaa aaggagaagg agcagaccca ggaagattac gacttgtgcc ccgataaagc
     2521 caacaaatga gccaatccag aagaccgggt aataccagcg cacccacgtg tattgtcgcg
     2581 tcccccagcg tataaaactc gtaatgcgtg tctctggcgg gctttccatg atgcacttcg
     2641 gctgaagata gatcttgtaa ccagcttttt tcacagaaat cgtcaggata agatcatcag
     2701 agaacgcggt tttccatttt tccttaatat ccagggtatc gaacactttt ttcctgatag
     2761 ccatcgatcc accccaggca aacgtataac tggggtaaaa cataaacaca atcgaagcca
     2821 tattccaagc agatataagc aatgttttcc agttcgtagg aaaataccat cggtatccgg
     2881 tcgttacccc gatggcctca tcttggagtg gcatcacaat gttctgaagc cagtgtgtat
     2941 ccggtttgat atcgctatcg gcaaacacca gaacctctgt tttttctgtt gacttaagtc
     3001 cagtgagcag cgcagcaatc ttcccgctac atccagaaac agaatcggtc agaaccaaat
     3061 gagcatatgg tttgttctct atgagttgtt gcaaggtggt ataggcagga tcgtctgttg
     3121 aatcgacgac aaatatgatc tcatatttct ggtattcctg ggcgagaaac ccttggatgt
     3181 tttccaaaaa accatgtccg gtccctttac aggggacaat gacagataca tagggaaaat
     3241 agggttgaaa tatccttttt tttgtccgcc gatacgcgat aagccacaaa ctgatgatat
     3301 aacattcacc aaggattcca atgattccta gaacaatcag aaattcaaga atcatgtctc
     3361 ttcaacaata gtttgtatcc ttcgctggtt tttagagatt tacctttcag gattttccca
     3421 aatccctacc agatacgtct gtaaaaggat ggggtttatt aaaagaaatc caatatatca
     3481 tcttgtgatt aatcacactg atacgaggaa agatagacaa tatctcaaag gactcgagaa
     3541 cattgatttc cgaccagttt ttatcctcgg ggttcatcga tccgggacca gtatcctgta
     3601 caagatgtta acagccaccg ggtgcttcaa tccagttacc gcgtatcatc tcatcaccta
     3661 tgacgagctc ctctccaact atcatgaaca aaaagaacat ggcgcaaaac aacagctcac
     3721 agaatcgttt ctcaaaaacg ggcttaagga cagaggaatc gaccggttaa agataaccgc
     3781 ggattttgct gaggaatatg gatttttact tggtaatcag acgctgcaga tgcgtatcac
     3841 gaaaaagaat gtcgcattgt tcattgagtt gtgtaaaaaa atccagttca tcgcaggaaa
     3901 caaaaaacct atcctgctaa agaacccgta tgatttccca aatttccttt atttgaaaga
     3961 ggtgtttccg aacgcacgat ttgttttcat ccatcgccat ccgctgaaaa caattagctc
     4021 aacgcttaat gctatcagga cgatagtaaa agaaagaaac ccgtacacca ccaggttatc
     4081 gaaggtctat gataagtttt attcgaatcc cttactgttg caaccgctcc gattcatcgt
     4141 tcatacaatg gcggaatgtt gcgtggttgt tcttacgaga attacgaaaa aagcggtaga
     4201 atattatgta aaaaacatag agaaattgcc gaaggatgat tacatttcaa tcacctatga
     4261 agatttctgt gtccatccac aagagacgtt acagaacatt atggataaat tgtccttgac
     4321 catgatacag caaatcgatg catcatcact aatgaacccg cgtaaagttg acatagatag
     4381 ctctgtagag aagctcagaa tatatatcta caaatccatg gaagagtatt tcgatctgtt
     4441 tcattatacg atagataggt agttgagaat tactttttgc ggtgttatgt tctgaattaa
     4501 aactcccccc gctactcgtt tcgtactagt cttttgttca taaaaccttg atattaagag
     4561 aatcggtgaa acggaaaatc ctgatacccg atgaccccaa tccaaatggt ttgattgaac
     4621 ccctggatgg tgacgtttga tcggaggatc acttgaagtt gatcagggat aaaccgttcc
     4681 ccacggcgcg gccagagttg agtcaagggg atgaacatat caccctggag taccgtagtg
     4741 ctctggggcg caataaaacc gtcagggagc gtcccattgc tgatcatgcg ggtctttgtc
     4801 ccatcgacac gatagatttg tacggtgatg tcattcgcaa gcaacgtcaa tttatttggg
     4861 ttttttatcg taaacgtaac ctcatcatgc aacccgtctt tcaaggtgta tgtataaaca
     4921 ccagtgagag atgcttcagt cgggagatcc gaaaggagct gtccaaggta aggggggatg
     4981 atgtcggcct ccaaggaaat gttcattggt tttctaatgc cagcgataga gatgataata
     5041 tcaccagtca gggtcatccg cagcgatact gcatttaatg ctttgaggag gatccatcca
     5101 ctcccggtga gttgtttggt tgtctttgca gggattgttt ctccttggat agtgacagtt
     5161 ccgacaggga ttcctgtttc agtcgttatg tctgctgaaa ggttttctac ggagatatcg
     5221 atcgtgttcg ggttggtgat ttcaataacc ccagtgaagt tgatgccttc ttgggtgata
     5281 tcactgaaat tcgccgcgag atggatatga ggtagtgtaa attgttccag gatgttattc
     5341 agcgatgttt caatggtgaa tttcaggggg aatgtttttt ttatgatgcc gaaaaatata
     5401 gtgccgaacg tcccggtcag acggctggta aggtgggtgg gtagagaacc gtcgaatcgg
     5461 atgtttgctg ttgtggtaaa cgatctgttc tcatgtgctt tggtttctcc tccgggcatc
     5521 gtaaggtggt tgatggtttc tccgttttca tcagttgtta tcatcgttat gttttggaga
     5581 atgacgggaa acgaattgtg gttgttgatg gagagggttg tctggagtac tgcttcatca
     5641 gtggtgatgg tgattatctc gagtgcgagt gtcgtttccg gtatgtccat ttgttgaatg
     5701 tcaaggaata gaaatgcgcc tgcgacgata ttcagaatcg ttgctgcgac aaggattatg
     5761 acgattattt ttttcatagc ttccgttttt gagagaaatc ctttatattt ataagatatt
     5821 ttctatatta atatactttt tctatagaat ataaaagcag ggttgatttt tcttcatata
     5881 ctcctgaaat gattgtgggg aaaatacttt gaaaaaaaga ggatagtacc tataattctc
     5941 cattatgttt tcatgctttt tcttttagaa taatacaaga tatctgtgtg cttcgtgtat
     6001 cccaattttt cgaacacctg catagaggtg gtattccaat cctcgatgag gcaggcaatg
     6061 atatcgattc ccattgatga aaaatgatgt tcgacttctt cgaccaaacg ctttgcgatc
     6121 cctttctttt gataggaggg tgcaatcacc agtcgattga tccatccttt ccgcccatca
     6181 tgtgttccaa caatcgcccc gattatcctt ttatcagtct ccgcgacaag aaaatagcag
     6241 ttcggttgtt gcaattgtat ggtgatattt tttctgctat cacggccttg tggtcgatag
     6301 gggatgttcc cttcagccca taaggcgatg accgaatcat aatctttaag agtcatttta
     6361 cgaagactca ctttggggct gtgtttcatc tttgatcttc cacaccttaa agggacagta
     6421 tgaaaagtat gaaccatgtt catttgaacg tttctatttg tctgatctct catctattac
     6481 gatcgactga tcaagaaaaa taaggagcag tttggtaaaa ttcttgttga agaggacagg
     6541 gtggaatcaa gggttcacat cacctgaggt ctgacgatcg tacggtaaag gataacattt
     6601 gatacgacca aaccgatgat gggatacagc acgaggaacc cagctaaggg tgcgacactc
     6661 atcaaaagaa tcggtatatt taaccagttg atcatatatt gaagaaccac atatttccgt
     6721 acttcctgtc gataaaagct tcttagatga accattttgt acgtgacgat gaaaaataat
     6781 acattaataa gaataagggg gagaattaac gaaatatcaa ggtgaatcac accaaaaaaa
     6841 ataggtagga acacacagaa cgcatacagt aatcccaaga ggtatgcgat gagttgaaat
     6901 gatgcgggaa tacggagggt tccctgctcg acccggacac ctagtcgcgt cgaaagatgt
     6961 tgagactgca tcaaataatc atggtctgca tctttgatcc ccccagcgac cagattcagg
     7021 ttgagggttt ggagaaacgc taagatgcag aggataatta ccagagatga gaattggaaa
     7081 gagacggtcg ctgcgccaaa caccacaccg accatgactg agacggcaag gaaaaaatcc
     7141 ataccacaaa ttcgtttgct atacaaatca tagaaccctg caaggagaat cccaaacaga
     7201 tacagaagta gcagtatgac accataaaaa taaagtgtca acaaacatcc aaggagtaaa
     7261 accagcagaa tgtaggcaag agcgtttctc ttcgagatac tgccactcgg tagtggtcgt
     7321 ttactggtag aagaaatgag acgatcgacc tcgacgtcga cataatggtt gagcgcaaaa
     7381 ccaacgatgt gagcagaaag accgatgaga aaaagcacag taagaaggaa taaatctttt
     7441 tcacccatcg caatcgcacc agtaagtggg atgattgcgg ttaaaaatat gaaatgagcg
     7501 ctggataatt tcgcgtattc aaaaacagtt gttttcaggt tcattagttc cttatccgcc
     7561 tgtttagcat catagaagag atatttatat aacttggggg cgacaccatt attatccagc
     7621 gtaaggcttg attttctctt acaaaaaggt agtaataata ctgagtcagt gatctaattt
     7681 cgaaatataa aaatcagttc cgcgacaggg ttagattcga tatatcttca tccttccatt
     7741 agaacggagt atgattttga atggtaatag taaatgattt attttccttg ttgcagtgat
     7801 aatttatatg atctgagaaa acaatcctat taagtaatcc aatccaatga gtgtgagcac
     7861 aagaaggagg acaatgagga gaatccatag agcgaatttg aaaagtttcc aagccacaat
     7921 accaatcaca agtattgcga tcagtgcaag ctatattgga attaccatcg ttcatcactt
     7981 tatttcttgg ttaataccaa tgatgctcct tggtcattgc tgaggatcag ctgatttgac
     8041 gaagggaaac tatagttcag cgtgatgccc gtatctcctg atgaaaacgt tattttctga
     8101 ttcgcaagag aataggcaaa caacccggtt agggagacga ttgcaagcgg accgctccca
     8161 ctgatcgtta ctttattatt cgtatcaaag gtcatcgatg tgcttccatc ttgagtttcc
     8221 catgttccta tgaatttgct tttttcatcc aggcagccgc ttaagtaact gatgctgagt
     8281 attgcaagaa ttcctattcc gatgaagagt ctcttcattt tttcctcatc aatcgaattt
     8341 cttcagatat ttataaagat tgtgtaaaca tgattttaaa aataagaaaa tagaaaaaaa
     8401 caggtattct caggtactct tacatgcgga caaggatcac gaagaacaag aacttaaatc
     8461 ccttcgcggt ctgtgtcacc gattcacccc cggacgtctg cgctgtgacc gtgatctcta
     8521 aacgacccaa tcctaggggg attcgatcgg ttatgaccgt ggactccgat tgcgtcgaca
     8581 acgagttaat cgtgccagtg gaacgaagat ctattcgttt gaggatccct ccggtgacgg
     8641 agatactcca attgacagat tctgcatttt gatct
//
LOCUS       DATMXJ010000039         6134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68529, whole genome shotgun sequence.
ACCESSION   DATMXJ010000039 DATMXJ010000000
VERSION     DATMXJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68529"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VN377_01150"
     CDS             <1..507
                     /locus_tag="VN377_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27021.1"
                     /translation="DEMYLEKEKQKKIGMADLANLFHSTLFSLERAIQETSGNTSMIV
                     TSHACKFLDKIEEKKDSKIFTRGTLEEAIDNFVEFTSGSDFFEHVEFKKINNEEYLFE
                     IKGCSLAKTGVHDTLNPEKDICPIALVAGALLKYYNPNSDVVVEPSKFTSTDIENRIH
                     CIKLGKCS"
     gene            629..964
                     /locus_tag="VN377_01155"
     CDS             629..964
                     /locus_tag="VN377_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWR27022.1"
                     /translation="MKSIFPQPIRNLPEITLPFNGVKGYLVQGEKEQVVFMEFEKDTS
                     VPEHSHESQWEIVLDGKVDYWQDGKKHTYTKGDRFFVEKGKKHSADVYAGYSCIMFFD
                     QKDRYSKKE"
     gene            complement(998..2035)
                     /locus_tag="VN377_01160"
     CDS             complement(998..2035)
                     /locus_tag="VN377_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27023.1"
                     /translation="MISKHTPLTVAWELTLTCNMHCMHCGSFAGETRAHELNTAEALD
                     LCSQLQQLHTKYINLTGGEALLRKDWYDISKEIKDRGMEVSILSNGLILNEKIITQLR
                     SVGAYAIAISLDGGTPQTHDMIRGVPGSFEKCLSGLKALKKEDLPATVITTVHKGNIQ
                     ELPLIRDTILEKTKAWQIQIAVPIGRFPIEFTLSKEEFYAVALFIAAMKKRYSAKELA
                     VMGAHSIGYHSQLLRNTMVSPVWNGCQAGLSVLGIQSDGTIKGCLSLPESYHEGNIRQ
                     RWLADIWNDPHAFSYTRNCSSSDLQDHCAACRFGKTCKGGCTTVSASLTGAPHKDPYC
                     LFSLEKKMLSG"
     gene            complement(2113..2406)
                     /locus_tag="VN377_01165"
     CDS             complement(2113..2406)
                     /locus_tag="VN377_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27024.1"
                     /translation="MYTCITLTYSIIRGKEEDMNKKNQHLTTTLQHMGQTLGLTDRDI
                     ANAKRTARSMLGITIAAAVVIIIGKIVTSQLDAVGQYYAGVSLKDFGLLSRFF"
     gene            2518..3054
                     /locus_tag="VN377_01170"
     CDS             2518..3054
                     /locus_tag="VN377_01170"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HWR27025.1"
                     /translation="MEHTINASTLILVQGDITKETTEAIVNAANGKLAGGGGVDGAIH
                     RRGGPTIMQQCRKIGGCPTGKAVITTAGDLNAKYVVHTVGPRYHDGTDNEAALLKSAY
                     HESLKLATTKHLTSIAFPAISTGAYGYPLHEAAKIALQTTIEYLTEHQDIQLVKFVLY
                     DHTTYDIFSQELKILLSQ"
     gene            3168..3416
                     /locus_tag="VN377_01175"
     CDS             3168..3416
                     /locus_tag="VN377_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27026.1"
                     /translation="MVHIKLKVGHSYELKINEQRTIVFAVLYGFERGRNKDIYTIRIY
                     DRKRDVEFPLEESTFDDWAKDGRIKELTAEEALAYVIG"
     gene            complement(3454..3672)
                     /locus_tag="VN377_01180"
     CDS             complement(3454..3672)
                     /locus_tag="VN377_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27027.1"
                     /translation="MTRFRTMKKKVIELFDEKTGGVVRTICFRNPKEFDEFVKGFKEM
                     RYPGYAWRYKDKGRKKGESDSADHYHET"
     gene            complement(3733..3969)
                     /locus_tag="VN377_01185"
     CDS             complement(3733..3969)
                     /locus_tag="VN377_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR27028.1"
                     /translation="MVIGFVLMHVSPLHEYEVYNALSKLPEVIELHPLFGEYDLIAKI
                     EAKDYDGIGEIIIQKIKTIEGITDTKTLTGIRLH"
     gene            4258..6129
                     /locus_tag="VN377_01190"
     CDS             4258..6129
                     /locus_tag="VN377_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWR27029.1"
                     /translation="MRKKLISIIFCMLFIIAAIVPVTGNLTFEASDDENNQTTTRGWI
                     TLPEPLPVDVLLEQTISRRMSFHNGYPPTPVTDEELSTVLWAAYGVTTTGGRTVYSPN
                     GTYSTTIYVIRSDATYIYVPSNHSLLLWKTGNYLYLGQDTGAPIKFGMIWNMSIVPDE
                     KAAMAEIGMIAQDVYFDANALNLATITTGMGVSDLYELDLPSNEKPEIIMHLGHPPTT
                     YNFVYNPLPPSSLPAVINNTLTLAEAVNTRTIVNEWNNTELTPLEQSQVLWASYGTSY
                     LYDNINHKRHRTLPSAINIYPFKIYAANQTGVYQYAPTTHSLSMIVSGDKRELIQDNV
                     DPGNISVSSAPLIIIPFWDKNIGSQTYLTWWWYESGAIIHNILLEATALDMSGNVLSV
                     ITDQNGLRTALGLSAQSNLVALHVAMVGHTGGGTQNNPPLAPTLTGPSNGQKGILYNY
                     TVVTTDPDGDDVFYFVDWADGTNSGWVGPFPSNSMQTLNHIWSQPGTYIIQAKARDTH
                     SLESSWTTLEMTIGGPVLELEIKGGLGITATVTNTGTADAINISWELAFEGGFVIPAQ
                     KTGTIPELLVGQQNTIHPWVLGLGKKTITMSLTADGGVTAEKIATGFFLMFIVVGVK"
BASE COUNT         1780 a         1528 c         1293 g         1533 t
ORIGIN      
        1 gatgagatgt atctcgaaaa agaaaaacaa aaaaagatag gaatggcgga tttagcgaac
       61 cttttccact cgacactttt ctcccttgaa cgagcaatcc aagagacaag tggcaatact
      121 tcaatgatag tgacctcaca cgcatgtaaa ttcttagata aaatagaaga aaaaaaagac
      181 tccaaaatat tcaccagggg aactctggaa gaagctattg ataattttgt tgaatttaca
      241 tctggcagcg acttctttga acacgttgag ttcaaaaaaa tcaacaacga ggaatatctg
      301 tttgagataa aaggatgttc gttggcgaag actggcgttc atgatacatt aaatcccgag
      361 aaagacatct gtcctatagc attggtggct ggtgcactcc tcaaatatta caacccaaat
      421 tcagatgtgg tagtcgaacc gtccaaattt actagtacgg atatagaaaa caggattcat
      481 tgcattaaac ttggtaaatg ttcttaatag tattttttcc atttctcttt cgttttacaa
      541 ttgaatataa gattatgatc ctctaaaaat tggtagagga taaaagataa aatatcccct
      601 ccttattctc ataaaaaggt gccagtccat gaaaagtatc tttccacagc cgatacgaaa
      661 cctcccggaa ataacactgc ctttcaacgg agtaaaaggc taccttgtcc aaggggagaa
      721 agaacaagtc gtgttcatgg agttcgaaaa agacacaagc gtcccagagc attcacatga
      781 aagccaatgg gaaatcgtct tagacggaaa agtcgactat tggcaggatg ggaaaaaaca
      841 cacgtataca aagggggata gattcttcgt tgaaaaagga aaaaaacatt cagcggacgt
      901 ctacgccggg tatagttgca tcatgttctt cgatcagaaa gacaggtatt cgaaaaaaga
      961 ataacatcgg ttcgttctgc tcttcggtcg aaattattca tccagacagc atttttttct
     1021 ccaatgagaa caggcaatag gggtccttat gcggagcacc ggtgagagac gctgatacgg
     1081 tcgtacatcc tcccttgcat gtttttccaa atcgacacgc agcgcaatga tcctgcagat
     1141 cggatgagct gcaatttctg gtgtacgaga atgcatgcgg atcattccag atatcagcta
     1201 accatctctg acggatgttc ccctcatgat acgattccgg gagggacaaa catcccttga
     1261 tggtcccgtc gctttgtatc cccaggacgc tgagtcctgc ctggcatccg ttccagaccg
     1321 gggagaccat ggtattccgt aggagctggg aatggtaccc gatgctatga gcgcccatca
     1381 ccgcgagttc cttggctgaa tagcgtttct tcatggcagc gatgaacagt gcaacggcat
     1441 agaactcctc cttggaaagc gtgaactcta tagggaatct tccgatgggg actgcgattt
     1501 ggatctgcca tgcttttgtt ttttcaagga tggtatctcg gatgagcggt aattcctgta
     1561 tgtttccttt atgaacggtc gtgatgacgg tggctgggag atcttctttt ttgagtgcct
     1621 ttaagccact gagacatttc tcaaaagaac caggtactcc gcggatcata tcgtgtgtct
     1681 gtggtgttcc tccatcgagg ctgatggcta tggcataggc tccgacgcta cgaagttgtg
     1741 ttattatttt ttcgttgagg ataaggccgt tggaaaggat gcttacttcc attccacggt
     1801 ccttgatttc tttgctgatg tcataccagt cttttcgcag gagtgcttct ccaccggtga
     1861 gattgatgta ttttgtgtga agctgttgca gctgggagca cagatccagc gcttcagctg
     1921 tgtttaattc atgcgctctg gtttctcctg cgaaagatcc gcagtgcatg cagtgcatgt
     1981 tgcaggtgag ggtcagttcc caagcgacgg tgaggggtgt gtgtttggat atcatagaag
     2041 catctctttg tgagaaaaca ggggggaagc actgctaatg ggggttttcc ttagcagtgt
     2101 cccttttttt tgttagaaaa acctgctgag caggccgaag tccttgaggc ttactccggc
     2161 gtagtactga ccgaccgcgt cgagttgtga tgtcactatt tttcctatga tgatgacaac
     2221 ggctgctgcg atcgttatgc ccagcatgct tcgtgctgtt ctttttgcgt tggcgatgtc
     2281 tctgtctgta agtcccagcg tctgccccat gtgctggagc gtggtggtga ggtgttggtt
     2341 ttttttgttc atatcttcct ccttgcctct tattatgcta tacgttagcg tgatacacgt
     2401 atacatttaa catttatata gttttcttac catccaaaaa tcctacctcc gaacacattt
     2461 tatagccaac ctcgataaat gaattgaaaa ctcaaagaaa aaaatggaag ataccaaatg
     2521 gaacacacaa tcaacgcatc aacgctcatc ctcgttcaag gtgacatcac caaggaaaca
     2581 accgaagcga tcgtcaacgc cgcaaacggt aagctagctg gaggtggagg cgtcgacggt
     2641 gcgatccatc gtagaggcgg accaaccatc atgcaacaat gcagaaaaat cggaggctgc
     2701 ccaaccggaa aagcagtcat caccaccgca ggcgacctca acgcaaaata cgtcgtccac
     2761 accgtcggac cacgatacca cgacggaaca gacaacgaag cagcactcct caaaagtgcc
     2821 taccacgaaa gcctcaaact cgccacgaca aaacacctca caagcatcgc gttccccgcc
     2881 atcagcaccg gagcgtacgg ttacccgcta catgaagcag cgaagatcgc cctgcaaaca
     2941 accatcgagt acctcacaga acatcaggac atccaactcg tcaaattcgt tctgtacgac
     3001 cataccacct atgatatatt ctcccaagaa ctgaaaatat tattatcgca atgaattcca
     3061 cattctagga ggaattaatt tcaggataaa gcactattta gacgccaata gctccttttt
     3121 taaacaaaga acagatatga aaacataacg tgaggaaaag agaacctatg gtacacataa
     3181 aattgaaggt cgggcattcc tacgaattga agataaacga acaacgaacg attgtttttg
     3241 ctgtcctata cgggtttgaa cgaggaagaa acaaggatat ctataccata cgaatctacg
     3301 acagaaaaag ggatgttgaa ttcccccttg aagaatccac ctttgatgac tgggctaaag
     3361 acggacgaat caaagagtta accgcagaag aagcattggc atacgtaata gggtgaggac
     3421 tgtacgaaac cccggatttt ctccttgaat aactcatgtc tcgtgataat gatcggccga
     3481 atcgctttcc cctttctttc tgcctttatc cttatatctc cacgcatacc cggggtatcg
     3541 catttcctta aaacctttta caaactcgtc aaactccttt ggattacgga aacagatggt
     3601 ccgtaccaca ccaccggttt tttcatcgaa taactcaatg accttttttt tcatcgtccg
     3661 aaatcgagtc attgcctctc cacacgtccc aacactatca tcaacacatt ccattttttt
     3721 aaatcagctg tgtcaatgca accgaattcc agtcaaggtc ttggtatcag tgatgccctc
     3781 tatcgtttta atcttctgaa taatgatctc tccgatgccg tcatagtcct ttgcctcgat
     3841 ttttgctatc aaatcgtatt caccaaataa gggatgaagc tcaataacct cagggagctt
     3901 cgacagtgca ttatacactt catattcatg caatggggac acatgcataa gaacaaaacc
     3961 aattaccatc gtgctacctc cgttctcata aaaaatccta acatctcgtt ccttaaaaac
     4021 atatcctcga aatcatcaaa ggtttttttt aaaaagtaac ctctttccgc tcataccttt
     4081 ccaatgcaat caaacatcta tgatttgtta tattatatac acaaaataag aatccttgaa
     4141 cctctccatt cataaaccaa tgctcacccc catgtgaaaa atatcctcag ttttatatca
     4201 aatcaacagt aattttatat aaaccatatg catagcaatt gtataagggg aaaaatcatg
     4261 agaaaaaagt tgattagcat catcttctgc atgctgttca tcatcgctgc cattgtaccg
     4321 gtcaccggaa atctgacttt cgaagcatct gacgacgaaa acaaccaaac caccacccga
     4381 ggttggatca ccctcccaga accactccca gttgacgtgc tactagaaca aacgataagc
     4441 cgcagaatgt cgttccataa tggttatcca ccgaccccgg taactgacga ggaattatcc
     4501 acagtcctct gggctgcata cggggtgacg accaccggtg gacggaccgt ctacagtcca
     4561 aatggaacat attcgacgac catatacgtg attcgtagtg acgcaaccta tatctatgtt
     4621 ccttccaacc attcgttact cttatggaaa accgggaact acctgtatct cgggcaggac
     4681 accggagcgc cgatcaagtt cgggatgatc tggaacatga gcatcgtccc tgatgaaaaa
     4741 gcagcgatgg cggagatagg aatgatcgcc caggatgtct atttcgacgc aaacgctctt
     4801 aatttagcga cgataacgac aggaatgggc gtcagtgatc tctacgaact cgacctgcct
     4861 tccaatgaaa aaccagaaat catcatgcat ctaggccatc cgccgaccac gtacaatttc
     4921 gtctacaacc cactcccacc ttccagcctc cccgcggtga ttaacaacac cctgacatta
     4981 gcagaagcgg tcaacacccg cacaatagtc aacgaatgga acaacacgga actcaccccc
     5041 ctggaacaat cccaagtcct ctgggcttcg tacgggacgt cctacctcta cgataacatc
     5101 aaccacaaac gacatcgcac cctccccagc gccatcaaca tctacccatt caagatttat
     5161 gcggccaacc aaaccggtgt gtaccagtac gcaccgacga cccactcatt atcaatgata
     5221 gtctccggcg acaaacgaga attaatacaa gacaatgtcg accccggcaa catctcagta
     5281 tcctcagccc ccctgattat catcccattc tgggataaga atatcgggag tcaaacctat
     5341 ctcacctggt ggtggtacga atccggcgcc atcatacata acatcctact ggaagcaaca
     5401 gcactggaca tgagcggaaa tgtgctctct gtcattaccg atcaaaacgg gcttcgcact
     5461 gccctgggtc tctctgcaca atccaatctc gtcgccctac atgtcgccat ggtcggccat
     5521 acgggtggag gcacccagaa caacccgcca ctagccccga ccctgactgg tccttcaaac
     5581 ggacaaaaag gaatccttta caattataca gtcgtcacga cagaccctga tggtgatgac
     5641 gtcttttatt tcgtagactg ggctgatggg acgaacagcg gatgggtcgg cccgttcccc
     5701 tctaactcga tgcagacact gaatcacatc tggtcgcaac ccggtaccta catcattcag
     5761 gcgaaagcaa gggacaccca tagtttagag agcagctgga ccacccttga aatgacgatc
     5821 ggtggacctg tactcgaact tgaaataaaa ggcggtctgg ggatcaccgc gacagttacc
     5881 aacactggca cagctgatgc catcaacatc tcctgggaac tcgccttcga aggtggattt
     5941 gtcatacccg ctcaaaaaac agggacgatt ccggaactcc tcgtcggcca acaaaacacc
     6001 atacatccat gggtgttggg ccttgggaaa aagacgatca cgatgtcgct taccgcagat
     6061 ggtggagtca ctgcagaaaa aatagcaacc gggtttttcc tcatgttcat cgttgtcgga
     6121 gtcaaataag accc
//
LOCUS       DATMXJ010000040         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68577, whole genome shotgun sequence.
ACCESSION   DATMXJ010000040 DATMXJ010000000
VERSION     DATMXJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68577"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VN377_01195"
     CDS             complement(<1..467)
                     /locus_tag="VN377_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWR27030.1"
                     /translation="MNTSDTFHDSLDYIHRKTKDILELVNKDDIENIIQSFFKADRIF
                     VYGAGRSGLVSKAFAIRLVHLGFQTFVIGETIGAPVKKGDLVCIVSGSGETIPAVMTA
                     EIAKNLGATLMVITGRKNSRITKFADIAIILSVDCTEKERKQFAPLGTLFEASS"
     gene            complement(464..1342)
                     /locus_tag="VN377_01200"
     CDS             complement(464..1342)
                     /locus_tag="VN377_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWR27031.1"
                     /translation="MIRIGQAGIPLSCKGRTNKDGIVYTHKSLDLNAIEIQFVRGLYV
                     LSDEEALFIKDYAVKNDIEIHVHAPYYLNLAGDREEITMSYEKIMKSAQMANNIGSKT
                     LIIHPGFYGEYNPKKTLKLITKNAKKIISMFKKEKLKVRLAMETMGKQKVFGSLDEII
                     EVCTHVKEIIPVLDLGHIHARGNGSIQEREDFEAIFDKLKPLRLRHFLIHITGVLYEN
                     GNELYHVPIKKGDMPLAPLIEVILDRNLNVTLISESPLLEHDAVYIRLQVEKAIMKRT
                     GKETAYYKDLSEVIKK"
     gene            complement(1403..1660)
                     /locus_tag="VN377_01205"
     CDS             complement(1403..1660)
                     /locus_tag="VN377_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HWR27032.1"
                     /translation="MPDDGKKIDQICDGLNMISEDNSIPRNIRRGAEEIKQILLKTDD
                     PIDLRVSSASSMLDELANDPNIPLHGRTLIWNIMSRLEELA"
     gene            complement(1716..>2033)
                     /locus_tag="VN377_01210"
     CDS             complement(1716..>2033)
                     /locus_tag="VN377_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWR27033.1"
                     /translation="IIMTQSAQETADFLAVMAQREQKEGDRAVAIRGEKTARTISEQQ
                     QFLVEGLPNVSAVLAQRLLQHFGSIRALANATEEDLCKITGIGKNIAADILKILNAEY
                     LKE"
BASE COUNT          484 a          406 c          456 g          687 t
ORIGIN      
        1 gagctagcct caaataatgt tccaagtgga gcgaattgtt ttcgttcttt ttctgtgcaa
       61 tctactgaaa gaataattgc gatgtcagca aattttgtga tccgagagtt ttttctcccg
      121 gtgataacca tgagtgtcgc accaagattc ttcgctatct ccgcagtcat gaccgcaggg
      181 atggtctccc cagaaccaga cacgatacat acaaggtctc cttttttgac tggtgcgccg
      241 atggtttctc caatgacaaa tgtctgaaat ccaagatgaa caagtctgat ggcgaaagct
      301 ttggagacga gaccagaccg tcctgctcca tatacaaaga tacggtctgc tttgaaaaat
      361 gattgaatga tgttttcaat atcgtctttg ttaaccagtt ctaggatatc ttttgtcttt
      421 ctgtgaatgt aatcaaggga atcgtgaaag gtatctgagg tgttcatttt tttatgacct
      481 cggagagatc cttgtaatat gcggtttctt tcccggttcg tttcatgatt gctttttcaa
      541 cttggagacg gatgtacacc gcatcatgtt caagtaaggg ggattcggag ataagagtaa
      601 cattgaggtt tcggtcaaga atcacttcga tgagcggagc aagcggcatg tctccttttt
      661 taatgggaac atggtacagc tcattgccgt tttcgtacaa gacaccagtg atgtggatga
      721 ggaaatgtcg taatcgtaat ggtttgagtt tgtcaaagat tgcttcgaaa tcttcacgtt
      781 cttggattga tccattcccc cgtgcgtgga tatgacccag gtcgaggact ggtatgattt
      841 ctttgacatg agtgcagacc tcaatgattt catcaaggct tccgaagact ttttgcttcc
      901 ccattgtctc catcgcgagt ctgactttta gtttttcttt tttaaacatg ctgatgattt
      961 tttttgcatt ttttgtgatc agcttcaagg tttttttagg gttatactct ccatagaatc
     1021 ctggatggat gatcagtgtt tttgaaccga tgttgtttgc catctgggct gatttcatga
     1081 ttttttcata actcatggtg atttcttccc gatcaccagc taagttgaga taatagggag
     1141 catggacatg gatttcgata tcatttttca cagcgtaatc tttgataaat aatgcctctt
     1201 catcggataa tacgtacaat ccacgaacga attgaatttc tattgcgttt aaatcaagac
     1261 ttttatgggt atagactatc ccatctttgt tggttcgtcc tttacaagat aatgggatgc
     1321 cggcttgtcc gatacgtatc atttctcttc ctcctctatt tgttctatgg acgaagggtc
     1381 gttttggtcc ttaaaaaata tgttatgcga gttcctccaa acgactcatg atattccaga
     1441 taagggttcg tccatggagt ggaatatttg gatcgtttgc aagctcgtca agcattgagg
     1501 atgccgatga gacacgcagg tcgattgggt cgtctgtttt taataggatt tgtttgattt
     1561 cttctgcgcc tcgtcttata tttctcggga tagaattatc ctcactaatc atatttaaac
     1621 catcacaaat ctgatcaatt tttttaccat catcgggcat ataaggtcac ccctaagctt
     1681 cacggtaatc acaaggtatg ataaaaatat ttgggctact ctttcaagta ctctgcattg
     1741 aggattttaa ggatatccgc tgctatgttt ttgccaatgc ctgtgatttt gcagaggtcc
     1801 tcctctgttg cgttggcaag ggctctgatg gatccgaaat gctgtaggag acgttgtgca
     1861 aggaccgctg agacattcgg gaggccttcg acgaggaatt gttgttgctc tgagatcgtg
     1921 cgtgcggttt tctcccctcg tatcgcgacc gctcgatctc cttctttttg ctctcgttgt
     1981 gccatgactg cgagaaaatc cgcggtttct tgagcggatt gtgtcatgat gat
//
LOCUS       DATMXJ010000041         6602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_888, whole genome shotgun sequence.
ACCESSION   DATMXJ010000041 DATMXJ010000000
VERSION     DATMXJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1622)
                     /locus_tag="VN377_01215"
     CDS             complement(105..1622)
                     /locus_tag="VN377_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HWR27034.1"
                     /translation="MFARKSLLIMSANLLDGLLAYIALYFISRDMGPEAYGIIGFAMG
                     FVGLFTILTDLGFNSAHIKRVSEGKDMGTCLGTYLISKLGFAGLFASIVVGIVLIWKF
                     IFGRGFESPTHELAIYIILGYYVVNSIGTFFYNTYSATKEIAKAQIPLVMGTVARTIA
                     ILFVALSGLGALALAWAYVFGEVIFLCISILFFRGFHIKKPTKECFKNYSVFAWPLIL
                     VGISYTIITNTDKVLIQLFWTSEEVGYYFASFRIVQFLLVAASAVGTLLLPTISGFHS
                     KEDINSIKKTVFLSERYISLLIFPMVIGIVVLAEPTVRILLSASFYPAVPILMVLPFF
                     VVLEALSQPYMYQVIGMNKPTLARNRILIILGLTVFFDLLLIPSNIKSLNLKLFGLGA
                     LGASIATVIGYAAGLIYCRYAAWKLAKNPLNPRILLHLLSASIMGFVIYELMRFLPIQ
                     RWYILIGYSLLGLGIYLFILFLLREFTKKDLDFVLDLFNVKKMWKYMREEIKKEK"
     gene            complement(1705..2910)
                     /gene="glmU"
                     /locus_tag="VN377_01220"
     CDS             complement(1705..2910)
                     /gene="glmU"
                     /locus_tag="VN377_01220"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HWR27035.1"
                     /translation="MKCVILAAGEGNRMHPLTFTRPKVMLPIVGKPILEWNLLNARAA
                     GLKEFVFIVSYKSEMVREYFGNGKPWNVKINYVNQGKALGTAHAIGTVEPFVKDFIVL
                     CGDTIFGINDIKQIAKKGTKIGLVKVDNATEYGIVELKGKHVLKIHEKMDHPISNIVN
                     AGTYHFNKQIFDYIRKTEKSPRGEFEITDSINMLVNKEHMEGVFLKEWRDVVYPWHLL
                     DANQELLKKMKTKIHGTVEKNVVINGTIIVGKSTIIRSGTYIEGPVLIGNDSKIGPNC
                     YIRPFTTIGNHCHVGNACEVKNSIIMDNSNVPHLNYVGDSVIGQNCNLGAGTAVANLR
                     LDKKNITVVLNGKKIDTKRKKLGAIIGDNVQTGINATINIGSMIGNDVYIGPGGFVKK
                     EVTPHTKIL"
     gene            complement(2924..4738)
                     /gene="glmS"
                     /locus_tag="VN377_01225"
     CDS             complement(2924..4738)
                     /gene="glmS"
                     /locus_tag="VN377_01225"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWR27036.1"
                     /translation="MCGIIGYVGYREARTVLLESLKRLDYRGYDSAGVAIIDKNLKVY
                     KEIGEIANVEKKIPQLKGTTGIGHTRWATHGGVTKENAHPHLSCDKKIAIVHNGIIEN
                     YTKLREELKKKGHRFISQTDSEVIAHLIDDAYKGNLEEALIAAIKKIKGSYAIVAVSQ
                     DEPGKIVGIRNESPLVIGVGDSENFLASDITAFLKYTNRVMFLNDGELCVITTNSIHV
                     FEKNKKQIKKKEELITWDFTDAEKSGFPHFMLKEIYDQPDTIHQVLRGRISEMEETIT
                     FPDHVEKLLRSEITSIHIVACGTSFYAGLIGKYLIEQLTNIPVFVEIASEYRYFGSRN
                     ETSLVIAITQSGETADTLAALREAQESGCKTLVITNVIGSTATRVADGYILQQSGPEI
                     GVAATKTFTSQIMVLFLITLKIALLQKKLGSDELYNYLFHLKALPRHVRDVLERSNDI
                     ITIAKQLKDAKSVFFIGRGINYPLSLEGALKLKEISYIHAEGFAAGELKHGPFALLTK
                     DTPVVAIVTLDETYEKILANIGEIKARGSPVIAIADDKDTDIENHADFVLRFPTSSHV
                     LPCIPITVVLQLLAYHVAHLRKCPIDKPRNLAKSVTVE"
     gene            complement(4740..5933)
                     /gene="glmU"
                     /locus_tag="VN377_01230"
     CDS             complement(4740..5933)
                     /gene="glmU"
                     /locus_tag="VN377_01230"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR03992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HWR27037.1"
                     /translation="MIKKAVILAAGEGKRLRPFTETMPKVMLPVANKPLLEYVFDATK
                     KSGIEEIIVIVGYKKEVIMEHFKDYKNIKITYVTQDRQLGTAHALLQAKKHIKDAFIV
                     LAGDNIIDAGSISKLIKDQSDYSLLIKEHPHPSKYGVVIVENRNISRIVEKPKEDIGK
                     YISTGIYKLPHSVFTDIERCTSEGAHALSSVIQSLIERGKHINTLLAKSWMDIVYPWD
                     LITVNETMIQKTSEYTSGTIEKGVTIKGPVSIGKETKIYAGCYIVGPVVIGDGCEIGP
                     HACIFPSTSIGKNTVVHPFSEIRSSVIMDDVHIASSAHISHSVIGKGCIIGDNFSTIS
                     GKAVIEIDNEFKKLETPIGTMMGEDCIIESHVVVDPGKIIGRKCKINPLKQIRKNIPS
                     ETKVM"
     gene            6192..>6602
                     /locus_tag="VN377_01235"
     CDS             6192..>6602
                     /locus_tag="VN377_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27038.1"
                     /translation="MRKSQLHVILTAGVVVLLLFSGCASGKINTSQSTTPDTSRQKNT
                     VTNDDARITCYVGGILHTRTISYESGMYLNELFSALVQANANDPCGKGTQMLKVRFIE
                     LLANLGLVSPHLSVQDVCSLIEPPWLHSALSLRRS"
BASE COUNT         1697 a         1307 c         1484 g         2114 t
ORIGIN      
        1 atagtgatct tttaaaacat caatgaaaat cctaaggacc gtgtttggat acccacagat
       61 gaggatgttt tttttcatta gtttcacgat tctaaaatat tttcttactt ttcttttttt
      121 atttcttctc tcatgtattt ccacatcttc ttaacgttga aaagatccag aacgaaatca
      181 aggtcttttt tagtgaattc tcttagcaag aaaaggataa atagatagat acctagtcca
      241 agcagagaat atccaatgag gatataccac cgttggattg gtaaaaatct cataagttca
      301 tagatgacaa atcccataat ggacgcagag agtaaatgga gcaaaatgcg agggtttaaa
      361 gggttttttg ctagtttcca ggcggcatat ctgcagtaga tgagtcctgc ggcgtaaccg
      421 atgacggttg caatggaggc tccgagtgct cctaacccaa agagtttcag attaagactt
      481 ttaatgttac taggaatgag aagaaggtca aaaaagacgg tgaggcctag gatgatgagg
      541 attctatttc gtgccagtgt cggtttattc ataccgataa cttggtacat atatggttgt
      601 gagagtgctt ctaaaaccac aaaaaaaggt aacaccataa ggatgggtac ggccggataa
      661 aatgatgcac tcaagagaat acggactgtt ggttcagcaa gaacaacgat accgatgacc
      721 atgggaaaga tcaacaaact aatatagcgt tctgagagaa agacagtttt tttgatactg
      781 ttgatatcct ccttgctatg gaatccagag atcgtgggga gaagcaaagt accaacagca
      841 gatgcagcaa caagcaggaa ctggacgata cggaaactcg cgaagtaata cccgacttct
      901 tctgatgtcc agaaaagttg gataaggact ttgtcagtgt ttgtaataat ggtataggag
      961 attcccacta agataagcgg ccaagcaaaa acagagtaat ttttaaaaca ttcttttgtt
     1021 ggttttttta tatgaaatcc tctaaaaaag aggattgaaa tacacaggaa gataacctcg
     1081 ccaaatacgt atgcccatgc tagggcaagt gctcctaacc ctgagagtgc gacaaagagt
     1141 attgcgattg ttcttgctac ggttcccatg acaagcggta tctgagcttt tgcgatttct
     1201 tttgtcgcag agtaggtgtt atagaaaaat gttccaatgc tgttgacgac ataatatcca
     1261 aggatgatat agatagcgag ttcatgagtg ggggattcga acccacggcc gaagatgaat
     1321 ttccagataa gaacgatccc gacgacgata gatgcaaata accctgcgaa cccaagttta
     1381 gaaataaggt aggtgcctag gcaggtcccc atatcttttc cttctgagac ccttttaata
     1441 tgtgcagagt taaacccgag atccgttagg atcgtgaaca atccgacgaa ccccatcgca
     1501 aacccgataa tcccatacgc ctcaggaccc atatcccgag aaataaaata taacgcgatg
     1561 taggcaagaa gaccatctag aagattggca ctcatgatta ataacgattt tctggcaaac
     1621 atcgaaaagt caaggaagta acaaaatgag atatattaaa caaatgggca cgggaaaacc
     1681 atagatttct tgctcttatt atggttaaag gattttcgta tggggggtga cttctttttt
     1741 aacaaaccct ccagggccga tatagacatc attacctatc atggaaccaa tattgatcgt
     1801 cgcattgatc cccgtctgga cgttatcgcc gataatagct cctaattttt tccgttttgt
     1861 atcaattttt tttccattta atacaaccgt gatgttcttt ttatcaagcc tgaggttcgc
     1921 caccgcggtt cccgctccaa gattacagtt ttgtccaatg accgaatcac caacgtaatt
     1981 aagatgtggg acgttactat tgtccataat gattgagttt ttcacttcgc aggcgttccc
     2041 cacatgacag tgatttccaa tcgtggtgaa tggtctgata taacaatttg gtccgatttt
     2101 gctatcattc ccaatcaata caggaccttc aatatatgtg ccggaacgaa tgatggtgct
     2161 cttccctaca atgatagttc cattaattac cacgtttttc tccacagtcc catggatttt
     2221 tgttttcatt ttctttaaca gttcttggtt tgcatcaagg agatgccatg ggtagacaac
     2281 gtctctccat tctttgagaa acacaccttc catatgctct ttgtttacta gcatattgat
     2341 ggagtcggtg atttcaaatt cacctcgtgg tgatttttcc gtctttctga tatagtcaaa
     2401 aatctgcttg ttgaaatggt acgtacctgc attgacaata ttggagatgg gatggtccat
     2461 tttttcatgg attttcaaca catgttttcc tttgagttct acaatgccgt attctgttgc
     2521 attatcaact tttactaacc ctattttggt cccttttttt gcgatttgtt tgatgtcgtt
     2581 gataccaaaa atggtatctc cgcagaggac gatgaaatct ttgacgaatg gttcgactgt
     2641 cccaatagca tgtgctgtcc ctagggcttt tccttgattg acataattta ttttcacatt
     2701 ccatggtttc ccgttaccaa aatattctcg taccatttca cttttataac tgacaataaa
     2761 aacaaactct ttcagtccag cagcacgagc atttaacaag ttccattcca ggatcggttt
     2821 tcctacaatg ggtaacatta cctttggtcg tgtaaaagtg agaggatgca tccgatttcc
     2881 ctcgcctgcc gctaggatca cacatttcat gggttttctc tccttattca acggttactg
     2941 attttgctag attacgtggt ttatcgatcg gacattttcg aagatgggca acgtgatacg
     3001 caagtaactg aagaacaacc gtgataggga tacaggggag cacatgggag cttgtgggga
     3061 agcggaggac aaagtcagca tggttttcta tatcagtatc tttatcatct gcgattgcga
     3121 tgacgggtga tccccgagct ttaatctccc cgatgtttgc cagaattttt tcataggttt
     3181 catccagggt gacgatagca acgacaggag tgtcctttgt gagaagggcg aatggtccat
     3241 gtttcagttc accagctgcg aatccttcgg catggatata ggatatttct ttcagtttta
     3301 atgctccttc gagggagagt ggatagttga tgcctcttcc aatgaaaaac acggatttgg
     3361 catccttcag ttgttttgcg atcgttatga tatcattgct ccgttccaga acatctcgga
     3421 cgtgtcttgg tagtgctttt agatggaaca gatagttgta gagctcatcg gacccgagct
     3481 tcttttgaag aagcgcaatt ttgagtgtta tgaggaagag aaccataatc tgggaggtaa
     3541 atgtctttgt tgcagccaca ccgatttcag ggcctgactg ctgcaggata tatccatctg
     3601 caacacgggt tgctgtactg ccgataacgt tcgtaattac aagtgtttta caccctgatt
     3661 cttgtgcttc acgtagcgca gcaagtgtat ctgctgtttc tccggattgt gtgatggcga
     3721 tgacaagtga ggtttcgttt cgtgatccga aatagcgata ttctgaagca atctctacga
     3781 acaccgggat gttcgtgagc tgttcaatga ggtattttcc aataagaccc gcataaaatg
     3841 aagtgccaca tgccacgatg tgaatcgaag tgatctcaga tctgaggagc ttttccacat
     3901 gatcgggaaa cgtgattgtt tcttccattt cagagatacg gcccctgagg acctgatgga
     3961 tcgtgtcagg ttgatcatat atttccttga gcatgaaatg agggaagcct gatttctcgg
     4021 cgtctgtgaa atcccaagtg ataagttctt ctttcttttt gatttgtttt ttattctttt
     4081 cgaaaacatg gattgagttg gtcgtgatta cgcagagttc cccatcattg aggaacatca
     4141 ccctattggt gtattttaaa aacgctgtga tgtctgaagc caggaagttt tcactatctc
     4201 ctacaccgat gaccaatggg ctttcatttc gtattccgac gatttttcct ggctcatcct
     4261 ggctgacagc gactattgca taggatccct ttattttttt tatcgctgcg attaatgcct
     4321 cctcgaggtt tcctttatat gcatcatcaa tgaggtgtgc gataacttct gagtctgtct
     4381 gggagatgaa tcgatgtcct ttctttttta gttcctctct gagtttcgta tagttctcaa
     4441 tgatgccatt atggacgata gcgatttttt tatcgcagct gaggtgtgga tgtgcgtttt
     4501 ctttggtcac acctccatga gtagcccagc gggtatgacc aattccggtt gttcctttca
     4561 gctgaggtat ttttttttct acgtttgcga tttccccgat ttccttgtag acttttaagt
     4621 ttttatcgat gatggcgaca ccagcagagt catatcctcg gtagtcgagt cttttcaatg
     4681 attcgagtaa gacggttctg gcttctcgat atcctacata gccgatgatt ccgcacacgt
     4741 tacatcactt ttgtttcgct tgggatgttt tttcgtatct gtttcaacgg gttgatttta
     4801 catttcctgc cgatgatttt tccagggtcg accacgacgt ggctttcgat gatgcaatct
     4861 tcccccatca tggttccaat gggtgtttct agttttttaa actcattgtc gatttctatg
     4921 actgcttttc ccgatatcgt cgagaaatta tccccgatga tgcatccttt tccgatgacg
     4981 gaatgggaga tatgggcaga tgatgcaatg tgtacgtcat ccatgatgac gctgcttcgt
     5041 atttcgctga atggatgtac gacggtgttc tttccgatgc tggtggatgg gaagatgcat
     5101 gcatgtggtc ctatttcaca gccgtcaccg atgacgactg gtccgacgat gtagcagcct
     5161 gcatagattt ttgtttcttt tcctatggag acagggcctt tgatggtgac gcctttttcg
     5221 atggttccac tagtgtattc gcttgttttt tggatcattg tctcattgac ggtgatgaga
     5281 tcccaggggt agacgatatc catccatgat tttgcgagaa gggtgttgat atgttttcct
     5341 ctctcgatta aggattggat gacggaagag agcgcatggg cgccctctga ggtgcatcgt
     5401 tcaatgtcgg tgaagacgga atgcgggagc ttgtagatgc cggtcgagat gtacttgccg
     5461 atatcttctt ttggtttttc tacgatgcgg ctgatgttcc ggttttctac tattacgacc
     5521 ccgtatttgg atggatgagg gtgttctttt atgaggaggg agtaatccga ttggtctttg
     5581 atgagttttg agatgcttcc tgcatcaatg atgttatctc ctgcgaggac gataaaggcg
     5641 tctttaatgt gtttttttgc ctggaggagt gcatgagcgg taccgagttg tctgtcttgg
     5701 gtgacgtagg tgattttgat gttcttgtag tctttgaagt gttccatgat gacttctttt
     5761 ttgtagccga cgatgacgat gatttcctca ataccgcttt ttttagttgc atcaaagacg
     5821 tattcaagca gtggtttgtt ggcgacaggg agcatgactt tgggcatggt ttcggtgaat
     5881 ggtctgagtc gttttccttc ccctgccgct aaaataactg ctttttttat catgattctc
     5941 acgagaccat atggttttgt tctttatttg acttttttct tttgatgatt tcttcatcac
     6001 ctttattatg cagtccttca ctataaagac ctgcacaggg aaaaaaatag ataaaatata
     6061 tagagcttga gagacgcaag gcgatgtacc atgatgaatt aaagtggaaa agaatatatt
     6121 tatatagagt attacatcta tatttaagga agaaacggtt tataaaatca tattgagaga
     6181 gggagaaaag tatgagaaaa tctcaattac atgttatttt gaccgctggt gtcgtcgtcc
     6241 ttctattgtt cagtggctgt gcgtctggga aaatcaacac gtctcaatcg acaactcctg
     6301 atacctcgcg acaaaaaaat acggtgacaa atgatgatgc aagaatcact tgctatgttg
     6361 gtgggattct gcatacccgg actatttcgt atgagtctgg tatgtatttg aatgagttgt
     6421 tctcagcgtt ggttcaggcg aacgccaatg atccctgtgg taagggtact cagatgttga
     6481 aggttcgttt tatcgagtta cttgcgaatc tcggtttagt ctcccctcat ctttctgtcc
     6541 aggatgtctg ttccctaatt gagccgccat ggttacactc cgcgttatcc ctgcgaagga
     6601 gc
//
LOCUS       DATMXJ010000042         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68695, whole genome shotgun sequence.
ACCESSION   DATMXJ010000042 DATMXJ010000000
VERSION     DATMXJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68695"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..472)
                     /gene="yciH"
                     /locus_tag="VN377_01240"
     CDS             complement(173..472)
                     /gene="yciH"
                     /locus_tag="VN377_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HWR27039.1"
                     /translation="MSDICPKCGLPKEICVCEEIAREQQSITIAVDKRRYGKTMTVVD
                     GLNPHDLDMNALVSNLKSICACGGTIKEGRIELQGDHRTRVKEALEKLGFTVDVI"
     gene            complement(469..672)
                     /gene="rpmC"
                     /locus_tag="VN377_01245"
     CDS             complement(469..672)
                     /gene="rpmC"
                     /locus_tag="VN377_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWR27040.1"
                     /translation="MALKVKELRQMNPEERSEKLRELKEELMHERGISAMGGSSPSPG
                     KIRQIRQSIARILTIMQEEGEHK"
     gene            complement(662..1537)
                     /locus_tag="VN377_01250"
     CDS             complement(662..1537)
                     /locus_tag="VN377_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWR27041.1"
                     /translation="MANERKFIKENAKRSLIKKFLVKEIEGAGFGGMSIQRTPIGTRV
                     NILVERPGMVIGKSGAKIKDLTDSIRTKFNVDNPQIEIQEAGSSASLNAQIMAEKLAE
                     ALERGWHFRRAGHSTVRRIMQSGAKGCQVILAGKLTGERHRTEKFTEGHVKYCGETAK
                     EVMDIGLATAKLKPGVLGVKVRIMRPDAKLPDEITYKIAVKEEVKPEIKVEAKPPEVI
                     LEKKTDIKNITKIPGIGPSVLKKLEKAKITSLEELYTMNLDDLIAIDGIGNKTAELIV
                     KNIKKLLEEGTENGS"
     gene            complement(1537..1983)
                     /locus_tag="VN377_01255"
     CDS             complement(1537..1983)
                     /locus_tag="VN377_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWR27042.1"
                     /translation="MPKYSAEKDSEKSARAYGYELHCSQKDSMNLAHALRGKTIEAAK
                     KYLEEIIAMKRALPAIYHKKKRLHQKGIGPGSFPEKAARYMLKIVENAENNAEYKGFD
                     VDKMKIVHISAYQGRIIRGIMPRAHGRATDKNETTTDVEIILEEVE"
     gene            complement(1990..2445)
                     /locus_tag="VN377_01260"
     CDS             complement(1990..2445)
                     /locus_tag="VN377_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWR27043.1"
                     /translation="MAQIKFGSAAKSRRKRERKKVEIRKKEFQYHGKTIEDLQKLSVE
                     ELLPMLTSRSRRTLKRGLSKDENKLMNDIKSAKQGDIIKTHCRNMIVLPSFVGHNILI
                     HNGKDFERVNIQPQMIGHYLGEFALTRKKVKHTGPGVGATRGSKFMPLK"
     gene            complement(2451..3158)
                     /locus_tag="VN377_01265"
     CDS             complement(2451..3158)
                     /locus_tag="VN377_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012193881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWR27044.1"
                     /translation="MGKNLIQQRRGRVRGRYNVPSHKYRGNIHYTNDRNTKGVVEGLI
                     HDPGRSAPVAQVRMENNKKVLMIATEGTSVGHEVKFTSSKADCELGNIMPIGNIPEGF
                     PIYDIEIKPGDGGKMVRAGGSGAYVISHDTHKTTIRLPSGQFKTLDSLCRATVGVAAG
                     GGRQDKPFMKAGKKWHASRGKGRNYPVVRGVAMNPVDHPHGGGSHQHVGKPSCVPRGA
                     PPGRKVGNIAARRTGRR"
     gene            complement(3170..3427)
                     /locus_tag="VN377_01270"
     CDS             complement(3170..3427)
                     /locus_tag="VN377_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWR27045.1"
                     /translation="MDPYSIVLYPFVTEKSMSHMEKNNALEFVVKRTANKELIKKAIE
                     KMFSVKVKSVNTRITNKGKHATVVFMPEYKAEDLGMRIGVF"
     gene            complement(3433..>3664)
                     /gene="rplD"
                     /locus_tag="VN377_01275"
     CDS             complement(3433..>3664)
                     /gene="rplD"
                     /locus_tag="VN377_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWR27046.1"
                     /translation="HVRAGKGKMRGRRYRVPKSLLIVSLKEGIEKSSENLSGVDITKP
                     QQLNIELLAPGGIAGRLTVFTKSALTKVGGAK"
BASE COUNT          759 a          878 c          745 g         1282 t
ORIGIN      
        1 tatgaggaag gtttgttgcg tctcatcgat gatgaggccg gtttgatgca cccatgatgg
       61 gtcggtgcat tcgatgatgc tgaccgttct accgatgagt tcatcgctag tgatcggctc
      121 gtgttccatt gaaggtcctt ccgtgacgca ccagatgcgt cagatgggtt tcttagatta
      181 cgtcaactgt gaatccgagt ttttctaatg cttcttttac ccgtgtccga tggtctcctt
      241 gaagttcaat cctgccttcc ttgattgttc caccgcaggc gcagatcgat ttgaggttgc
      301 tgaccaatgc gttcatgtca aggtcatggg ggttcagacc gtccacgacg gtcatcgtct
      361 ttccatatcg tctcttgtcg acggcgatgg tgatgctttg ctgttcacgc gctatctctt
      421 cacagacaca gatttctttt gggaggccac attttgggca tatatcactc atttgtgttc
      481 tccttcctcc tgcatgatcg taagaatcct tgcaatggat tgccgtatct gcctaatctt
      541 accaggggac ggtgatgatc cccccatggc agatatgcca cgttcatgca taagttcttc
      601 tttcaattct ctgagttttt cactccgttc ttcggggttc atctgtctga gttctttaac
      661 cttaagagcc attttcagta ccctcttcta ataacttttt aatgtttttt acaatcaatt
      721 cagcggtttt atttccgata ccatcgatcg caataaggtc atcgagattc atggtgtaga
      781 gttcctcaag ggaggtgatc tttgcttttt cgagtttttt cagtaccgat ggtccaatcc
      841 ctggaatctt tgtaatattc tttatatcgg tttttttctc tagtattact tctgggggtt
      901 ttgcttcgac ttttatctct ggttttactt cctcttttac agcaatttta taggtaattt
      961 catcagggag ttttgcatcc ggtctcatga ttctgacttt cactccaaga accccaggtt
     1021 ttaatttcgc agttgctagg ccgatatcca tgacttcttt tgcagtttca ccacaatatt
     1081 ttacatgtcc ttccgtgaat ttctccgtcc ggtgtcgttc tcctgtcaat ttgcctgcga
     1141 gaatcacttg acagcctttt gcgccagatt gcataatccg tctcaccgta gagtgtccgg
     1201 cccgtctgaa atgccatcct cgttcgagtg cttccgcaag tttctctgcc ataatctgag
     1261 cgttaagaga tgcgcttgag cctgcttcct ggatttctat ttgtgggtta tcaacgttaa
     1321 atttagtacg aatggaatcg gtcagatctt tgatttttgc cccactcttc ccaatgacca
     1381 tacctggtcg ttccacaagg atgttgactc tggtgcctat tggggttcgc tggatgctca
     1441 tcccgccgaa accagctcct tcgatttctt tgacgaggaa cttttttatc aaggatcgct
     1501 tagcattctc tttgataaac ttgcgttcgt ttgccattat tctacctctt caagaatgat
     1561 ttcgacatcg gtcgtcgttt catttttatc ggtcgccctg ccatgcgctc gcggcatgat
     1621 tcctcgaata atccttcctt gataggctga gatatggaca attttcattt tatcaacgtc
     1681 aaacccttta tattccgcgt tattctccgc attttcgacg atcttgagca tgtatcgcgc
     1741 agctttctct gggaagcttc caggccctat ccccttttga tgcaatcgtt tctttttatg
     1801 atatattgca ggaagcgctc gtttcattgc gatgatctct tcgaggtact tttttgctgc
     1861 ttcgattgtt ttccctcgta acgcatgggc aaggttcatc gagtctttct gggaacaatg
     1921 cagttcgtat ccataggctc gtgcgctttt ttctgagtct ttctcagctg aatattttgg
     1981 catggctcat cacttcaatg gcatgaattt agaacctctg gtagctccaa caccaggtcc
     2041 tgtatgtttt acttttttac gggttaatgc aaattctcca agataatgcc cgatcatctg
     2101 gggctgaata ttcactcgtt cgaaatcttt tccattatgg ataaggatgt tgtgaccaac
     2161 aaaagaaggt aaaacgatca tatttctgca atgggtcttt attatatccc cttgttttgc
     2221 gctcttaatg tcattcatta atttattctc atctttggat aatcctcgtt ttaacgttct
     2281 acgggatcgt gaggtaagca tggggagtaa ttcctcgaca gagagttttt gaagatcctc
     2341 aatggttttt ccatgatact ggaattcctt ttttcgaatc tcaacttttt ttctctctct
     2401 tttccgtctg cttttcgctg ctgatccaaa tttgatttgt gccatggtga ttaccttctt
     2461 ccagttctcc gggcagctat gtttccaact tttctaccag gtggtgcacc acgtgggaca
     2521 caacttggtt ttccaacatg ttgatggctg ccgcccccgt gaggatgatc caccgggttc
     2581 attgcaacac cgcgtacaac tggatagttt cttccttttc cgcgtgatgc gtgccatttt
     2641 ttccctgctt tcatgaacgg tttatcttgc cgtccaccac cggcagcaac tccgacggtt
     2701 gctcgacata aagaatctaa tgttttgaat tgacctgagg gaagacgaat ggttgtttta
     2761 tgtgtatcat gggagatcac atacgcgcca ctcccaccgg ctcgaaccat tttaccgcca
     2821 tctccgggtt tgatttcaat atcataaata gggaatcctt ctggaatatt tccaattggc
     2881 atgatgttcc caagttcaca gtcagcttta cttgatgtga atttcacctc atggccgacg
     2941 gaggttcctt cggttgctat cataagaacc tttttgttat tctccattct cacctgagca
     3001 acaggagcgc ttcgtccagg gtcatgaata agaccttcca caacaccttt tgtgtttctg
     3061 tcattcgtgt aatgaatatt cccacggtat ttatgtgacg ggacattgta ccgacctcga
     3121 acccgccctc gacgttgctg gataagattt ttccccattg ttcacccctt tagaagactc
     3181 ctatacgcat accgagatcc tctgctttat attcaggcat gaagacgaca gtcgcgtgtt
     3241 ttcctttgtt tgttatccgc gtgtttaccg attttacttt tacagaaaac atcttttcaa
     3301 ttgctttttt tatcagctct ttattcgcgg ttcttttcac cacaaactcc aatgcattat
     3361 ttttttccat gtgactcatt gatttttctg tcacaaacgg atagaggacg attgaatatg
     3421 gatccatggt cattatttag cgcctcccac ttttgttaac gcggatttag taaaaaccgt
     3481 cagtctaccg gcaatcccgc ctggtgcaag aagctcgata ttgagttgtt gtggttttgt
     3541 gatatcaaca ccgctgagat tctcggagct cttttctatc ccttctttta atgacacgat
     3601 gagtaaggat tttgggacgc gataccgccg gcctcgcatt tttcctttcc cggcacgtac
     3661 gtgt
//
LOCUS       DATMXJ010000043         5393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_63706, whole genome shotgun sequence.
ACCESSION   DATMXJ010000043 DATMXJ010000000
VERSION     DATMXJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63706"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VN377_01280"
     CDS             <1..1004
                     /locus_tag="VN377_01280"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR01213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HWR27047.1"
                     /translation="IDLITKTLEEYEFNTFLIGSKIDEDIQEKEQRLWDHLSLEDAEP
                     IKMEINREIGKILEPQLKKTVDIPNPGIMAVIDTTYDLVTLQISSLFIYGRYRKLQRG
                     IPQTKWPCQICLGKGCKRCNYTGKLYETTVEELVAKKALELTKGTDESFHGSGREDID
                     ARMLGTGRPFILEIKNPKKRNIDLSFLEKETNNALKNIIEVSCLRFSNREEVARIKAA
                     EFRKTYRVTIEADQPLNKEKLIKVAQSLQGQLIKQLTPTRVAHRRANKIRERLIYNCN
                     IESVEGLIASLTLEAESGTYVKEFVSGDNDKTQPNLSELIGIPCKVKELDVIDVKGE"
     gene            1011..1301
                     /locus_tag="VN377_01285"
     CDS             1011..1301
                     /locus_tag="VN377_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HWR27048.1"
                     /translation="MKRSKGTRSKSRYIMRKKPRTRGVSSSTRALQQFEVGASVSIDI
                     NSAVHKGMPHPRFQGHTGKVEGMQGNAYLVGLTVGKKHKTIVVLPEHLGRVL"
     gene            1302..1646
                     /locus_tag="VN377_01290"
     CDS             1302..1646
                     /locus_tag="VN377_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HWR27049.1"
                     /translation="METSTEETGKIVSLAEVKNILKKISKERKELLYEQKIALEHAEK
                     FAKLSAKQTKDLIVELMKLDHVEELQAYKIADILPSTEDDIRAIFAKERYTPNDKEIK
                     NILEIVKKHSVE"
     gene            1665..2237
                     /locus_tag="VN377_01295"
     CDS             1665..2237
                     /locus_tag="VN377_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HWR27050.1"
                     /translation="MEDYVYVLDYLAKGRGDLPAFKRNPVVYGIGESQYTFLELIPKR
                     DATFVTGERIYVGKDPTLRTKIEKIKGRINFEDLTSTAHGELPYVLLDIVHNNEARYV
                     KFFNEASAISTRYHVLELLPGLGKKMMLEILDERKKKPFTTFKEMQERIDFLRSPDKL
                     ISKRIELELSDPDQKYRIFTRLPLSRDHQT"
     gene            2240..2986
                     /gene="rsmA"
                     /locus_tag="VN377_01300"
     CDS             2240..2986
                     /gene="rsmA"
                     /locus_tag="VN377_01300"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWR27051.1"
                     /translation="MVKPRLGQHFLIDSSVAQREVVYAGITKNDVVLEIGPGKGIITC
                     ILAEKAKEVIAIEIDTHLVEYLKNILPENVTVICADALAVDFGSLPRFTKIVSNLPFE
                     ISSPLMFKFLQSSFSKAVLIFQKDFAERLVASPGTKEYSRLTVGVLYKAQCRLLETVP
                     RTCFSPQPKVDSSIVEIIPYHKPRFTLENEQFFFELTKQLFNHRRKKIRSTIKSIYGN
                     IEQLPFLDQRVEELTPEQIGQLSDQLSHSI"
     gene            complement(3068..3253)
                     /locus_tag="VN377_01305"
     CDS             complement(3068..3253)
                     /locus_tag="VN377_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27052.1"
                     /translation="MFKSVHLILFGGYDGVPFPFNSLMGYLSGGFDRLAADLAVSPLH
                     PAHFIILFLVFCNIDDP"
     gene            3442..>5393
                     /locus_tag="VN377_01310"
     CDS             3442..>5393
                     /locus_tag="VN377_01310"
                     /inference="COORDINATES: protein motif:HMM:NF010337.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR27053.1"
                     /translation="MYLHHDFIRSDLVERREYQWNIANSAANASTLVVLPTGMGKTII
                     ALLVIAKELAKEKNIVFLSPTKPLVNQHAQSLTAFLTLSDSIAVFTGEVPPEKRVALW
                     NSKRIIVSTPQVIENDLIAHRYDLSNISRIIFDEAHRATGNYSYVFIAEMFKKQQNER
                     RCLGMTASPGANLSEILEVCKNLEITNIEIRTKIDPDVRPYVHDLEIKWKEIPLPTEF
                     NYTLQLLKKALSERLTTLKNIGVLDSASVSLINRKKLLEIQQTIQSELRSRPQPPQEL
                     FKAASAQNAAIKLVHAIELLQTQGVNALQSYIARLTNEANSKGGSKASRDILRDPNVL
                     EAIAYIKSLKIEHPKIQEIVTIIKDQFSHKKDSKIIVFTHYRDTSQYVLKQLEELPFV
                     KPVRFIGQAGKDNDKGLTQKEQIDIIKRFKQDEFNVLIATSVAEEGLDIPSTDLVVFY
                     EPVPSEIRTIQRKGRTARQMAGKVIILIAKGTPDEAYYWSSRQKERRMHQELEVLRNA
                     LRKKIQDPTAIYQTALHQENQKKLDEFPKKSSLRIIVDHRESRSPVMRFLTQKDILVE
                     PQQLDVGDYIISSRIGIERKTVDDFLGSLIEGKLFVQMKNLRATYSRPLLLIEGEGLL
                     TKRNMSHNAILGSIAAIIVDFGIPII"
BASE COUNT         1788 a         1187 c         1139 g         1279 t
ORIGIN      
        1 ttatagacct gataacaaaa accttggaag agtacgagtt taacacattc cttatcggat
       61 caaagataga tgaagacatc caagaaaaag aacaacggct ctgggaccat ctatcacttg
      121 aagacgcaga acctatcaaa atggagatca acagagagat tgggaaaata cttgaaccgc
      181 aattgaagaa aaccgtggat attccaaacc ctggcatcat ggcagtcatt gatacaacct
      241 atgacctagt gaccttacag ataagttctc tcttcatcta tggacggtac aggaaattac
      301 agcgaggcat ccctcaaacg aaatggccct gtcaaatctg tcttggaaaa ggctgtaaga
      361 gatgtaatta cacaggaaaa ctctatgaga ctaccgtcga agagctcgtt gccaagaagg
      421 cattggaact gaccaaaggg actgatgaat cattccatgg gagtggacgg gaagacatcg
      481 atgcccgtat gcttgggacc ggacgcccat ttatccttga gatcaagaat cctaagaaaa
      541 gaaacatcga cctttccttc ttagaaaaag agacaaacaa cgcattaaaa aatatcattg
      601 aagtctcctg cttacggttt tccaatcgag aagaggtcgc tcgcattaag gcagcggaat
      661 ttcgaaaaac ctatcgggtt accattgagg ctgatcagcc tttgaataaa gaaaaactta
      721 taaaggtggc tcagtcatta cagggtcaac tcataaagca gttaacacct actcgagtgg
      781 cccatcgtcg ggccaacaag attagagaac gactcatcta caactgcaac attgaatcgg
      841 tagagggtct catagccagc ttaacgcttg aggcagaatc cggtacctat gtcaaggaat
      901 tcgtctctgg tgataacgac aaaactcaac cgaacctcag tgaactcata ggcattccct
      961 gtaaggtaaa agaactcgat gtaatcgatg tcaaggggga ataacaaaat atgaaacgat
     1021 caaaaggaac ccgaagtaaa agcagatata tcatgagaaa aaaaccgcga acacgcggag
     1081 tttcctcttc aacaagagcg ttacaacaat ttgaagtagg tgcaagcgtc agcattgaca
     1141 ttaattcagc ggtacacaaa ggcatgccac atcctcgatt ccaagggcat accggtaaag
     1201 ttgagggtat gcaaggaaac gcgtatctgg ttggtttgac tgttgggaaa aaacataaaa
     1261 ctattgtcgt gctgccagaa catttgggga gagttctcta aatggaaaca tcaaccgaag
     1321 aaaccggtaa aatcgtttcc cttgcagaag taaaaaacat tcttaaaaag attagtaaag
     1381 aacgaaaaga attgctgtat gagcagaaaa tagcgttgga gcatgcagaa aaattcgcaa
     1441 aactctctgc gaaacaaacc aaagacctaa tcgtcgagtt aatgaaactt gaccatgtcg
     1501 aagaactcca ggcgtataag atagcggata ttctcccaag tacagaagat gatattagag
     1561 ctatttttgc taaagaacga tacacaccaa atgataaaga aataaaaaat atccttgaga
     1621 ttgtgaaaaa acattcagtc gaatagtggt gggggtagga aaaattggaa gattatgtct
     1681 atgtcttgga ttacttggca aaaggacggg gggatctccc agccttcaag agaaatccag
     1741 tagtgtatgg tattggtgaa agccagtaca catttcttga gttgatacct aaaagagatg
     1801 cgacatttgt cacaggtgaa cgtatctatg tcgggaaaga tccgacactc cggaccaaga
     1861 ttgagaagat taaaggtcgc attaactttg aggatctcac ctccaccgcc catggggaac
     1921 tgccgtatgt cttattggat attgtccata acaatgaggc acgatatgtg aaatttttca
     1981 acgaggcttc tgcaatatca acacgctacc atgtccttga gctcttacca ggtctgggga
     2041 agaagatgat gcttgaaatc cttgatgaac ggaagaagaa accattcacc acctttaaag
     2101 agatgcagga gcggatcgat ttcctcagat ccccggataa acttatctcg aagagaatcg
     2161 aattagagtt atcggacccg gatcagaagt accgtatctt cacgcgtctg ccattgagcc
     2221 gggatcatca aacctgagta tggtaaaacc acgtctaggg cagcattttc ttattgactc
     2281 atcggttgct cagcgtgagg tcgtttatgc ggggataacg aaaaacgatg tggtccttga
     2341 gatcggccca ggaaaaggga tcatcacttg tatattagca gagaaagcga aagaagtcat
     2401 cgcaatagag atcgacactc acctcgtcga atatttaaag aacattcttc ctgaaaatgt
     2461 gacagtgatt tgcgcagatg cacttgctgt tgattttggt agcctcccga gatttaccaa
     2521 aatcgtctca aacctccctt tcgagatttc atcacctctt atgttcaagt tcctgcaatc
     2581 gtcattttcc aaagcggttc tcatttttca aaaagatttt gcagaacgtc tcgttgcttc
     2641 tccagggaca aaagagtatt cccgactgac ggtgggcgtt ttatataaag cccagtgtag
     2701 gttattagaa acagtgcctc ggacctgttt ttcccctcag ccaaaggtgg attcctcgat
     2761 cgtcgagatc ataccatatc acaaaccaag gtttacgctt gagaacgagc agtttttttt
     2821 cgaactgaca aaacaattgt tcaatcatcg aagaaaaaag atacgatcca cgatcaaatc
     2881 gatatacggg aacatcgaac aactcccgtt ccttgatcaa cgagttgagg agcttacccc
     2941 agagcagatc gggcaattaa gcgatcaatt atcccattct atctgatgcg cccagcttct
     3001 atttcatttt ttttaaagaa aaggggaaac aaggttctgt tgaatccagc taaacactcc
     3061 gacgaaatca aggatcatca atattgcaaa aaacaagaaa aagaataata aaatgcgcag
     3121 ggtgaagcgg agaaactgcc aaatcagcag caagacgatc aaaaccacca gaaagatagc
     3181 ccataaggga attgaaaggg aaaggaactc catcgtaacc gccgaaaaga atcaaatgaa
     3241 cacttttaaa catttggaaa aacgcataaa atttcgtggt agaaaccatt aaaaaatcat
     3301 tagggatgag aacacctggc cacgatgatg aaacactatc tcacactaag tgctgcaaca
     3361 tgctgagaaa tgggcgaact gagtggtcat gacatcgcgg tattcccaaa catacaaaaa
     3421 ctctgattcc atgtcttttt tgtgtatctt catcatgact ttatcagatc agatttagtt
     3481 gaacgacggg agtaccaatg gaatatcgcg aactcagcgg caaatgcaag cactctcgtc
     3541 gtgctcccaa caggtatggg aaaaacaatc atcgcacttc tcgtcatagc aaaagaactg
     3601 gcgaaagaaa aaaacatcgt gttcctctct ccgaccaaac ctcttgtaaa tcagcatgct
     3661 cagtcattaa ctgcttttct tacactctct gattctattg cggttttcac aggggaggtt
     3721 cctccagaaa aaagagtagc tctctggaac agcaaacgta tcattgtttc cacccctcag
     3781 gttatagaaa acgatttgat cgcccatcga tatgatcttt cgaacatatc tcgaattata
     3841 tttgatgaag cccatcgtgc gactgggaat tactcttatg tttttattgc agagatgttt
     3901 aagaaacaac agaatgaacg acgttgtctt gggatgaccg catcccctgg ggctaacctc
     3961 tcggaaatcc ttgaggtctg taagaacctt gaaatcacca atatcgaaat acgaacaaaa
     4021 atagatcctg atgtccgacc atatgtccat gacctcgaaa tcaaatggaa agaaataccg
     4081 ctccctactg aatttaatta tacgctgcag ttactcaaaa aagcactctc tgaacggctg
     4141 actaccctga aaaacatcgg tgtgcttgac tcggcatctg ttagccttat caatcggaaa
     4201 aaacttctcg agattcaaca gactatacaa tctgagcttc gctccagacc acaaccacct
     4261 caagaactgt ttaaggctgc atcagcgcaa aacgcagcga taaagctggt gcatgctatt
     4321 gagctgttac agacgcaagg ggtcaacgcg ttacagagtt acatcgcccg tctgacgaat
     4381 gaggcaaact caaaaggcgg aagtaaagca tcacgggata tcttaaggga tccaaatgtc
     4441 cttgaagcga tcgcgtatat caaatcactg aagatcgagc atccgaaaat ccaggaaatc
     4501 gtaactatta tcaaagatca gttttctcat aagaaagatt cgaaaatcat tgtctttacc
     4561 cactataggg atacctcaca atatgtcctc aaacagttag aggagttacc gttcgtaaag
     4621 ccagttcggt tcatcggaca agctggaaaa gacaacgata agggtctgac acaaaaagaa
     4681 caaatcgaca tcatcaaacg ctttaaacaa gatgaattca atgtccttat cgcaacctca
     4741 gttgctgagg aaggacttga tatcccctcg acagaccttg tggttttcta tgagccggtc
     4801 ccctcagaga tacgcacgat tcagcgaaaa ggcaggaccg ccagacagat ggcgggaaaa
     4861 gtgatcattc tcattgcgaa aggaaccccg gatgaagcat actactggtc aagccgacaa
     4921 aaagaacgca gaatgcacca ggagctagag gtattacgaa acgcgctgcg gaaaaaaatc
     4981 caggacccaa cagcgattta tcaaaccgct cttcatcaag aaaaccagaa aaaactggat
     5041 gaatttccaa aaaaatcctc cctaaggatc atcgttgatc accgggaatc ccgctccccg
     5101 gtcatgcgat ttctcaccca aaaagacatc ctcgtcgaac cacagcaact tgatgtgggg
     5161 gattatatca tctcttctcg catcggcatc gaacggaaaa ccgttgatga tttccttggc
     5221 tcactgatcg aaggtaaact cttcgtccaa atgaaaaacc ttcgagcgac ctactcccgg
     5281 ccactcctac tcatcgaggg agaagggctc cttacgaaga gaaacatgag tcataacgct
     5341 atccttggga gtattgcagc tattatcgtc gactttggca tccccatcat cat
//
LOCUS       DATMXJ010000044         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_74176, whole genome shotgun sequence.
ACCESSION   DATMXJ010000044 DATMXJ010000000
VERSION     DATMXJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74176"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1007
                     /locus_tag="VN377_01315"
     CDS             9..1007
                     /locus_tag="VN377_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR27054.1"
                     /translation="MFIIETKNLTKKFDDFLAVDHIQFSVEKGEIFGFLGPNGAGKTT
                     TIKMLTTLLYPSEGSASIAGFDILKQRANVRENIGVVFQEPALDTELTGKENLDFHAR
                     MYGLGQETRKKRIMQVLTLVDLSDKKDVLVKHYSGGMKRRLEIARGLMHSPTVLFLDE
                     PTLGLDAQTRRAIWEYIKQLNEEEGTTIFLTTHYMDEADFLCDRIGIIDHGKILVMDN
                     VDTLKKLVGHDVITLSSSDSLALQKRLEQEPWVSNITPHASFLTVGVERGEEKIPTIF
                     DIARSINVSISSVDVRKPTLDDVFLYYTGRSMREQNPETPRSRPSKPERFHLRQGK"
     gene            1004..1774
                     /locus_tag="VN377_01320"
     CDS             1004..1774
                     /locus_tag="VN377_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27055.1"
                     /translation="MMNQKDLQAIYMLWLRQMKRFLRAKSRVLSTIVQPLFFLFILGF
                     GFNIAVFPDMRGSYINFLAPGIIAMAILFSSMFTGVSVLWDRQSGFLQEVLVAPVSRL
                     SIVIGRTLGGATIAMFQGFIIMIIALVLGVPVVDVQGLLLTVVIMVLLSFTAVGFGLI
                     LASKMRDFEGFQSIMSLMLMPLLFLSSAFYPINDSLPGWLQALSYCNPLFYMIDGMRG
                     SLTGVNNVLHPLVDLGIILSLCIIIMGLGTYFFNKSEI"
     gene            complement(1852..2709)
                     /locus_tag="VN377_01325"
     CDS             complement(1852..2709)
                     /locus_tag="VN377_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWR27056.1"
                     /translation="MTATIINGREIAKEIRSKITFDVQRLKKKYQREPLVVTIAIGEN
                     PSSELYLKLRTIACNEVGISSKILRFDANVSESTILQTIQSLNNDPSIHGIFIQYPVP
                     LHLSPYKLMQTVAAKKDVEGFNPENLGRTMIGDEVLVPCTPLSVLTILEHEHVHLQGK
                     DVVIVNHSTIVGKPLATLLLNRNATVSIAHIFTKNLKAYTSNADILITGAGVKHLITK
                     DHIKNKAIVVDVGIIETEDGIYGDVDFESVKEKAAVLTPVPGGVGPVTIACALSNMVK
                     SYKNCVENK"
     gene            complement(2740..>3352)
                     /locus_tag="VN377_01330"
     CDS             complement(2740..>3352)
                     /locus_tag="VN377_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWR27057.1"
                     /translation="LTKGTVIAIDGSLSLLTFAHKILNEYTNVAFCTGDAQQLCFADD
                     TFDLITCNLLLMWADDPQMVLNEMTRVVKPKGKILASLEPDYGGKIHWPENKKVDPLF
                     AGEAIRKKGGDPHIGRKLRKLFITAGLDTVIGIGNNRIWSCEEDKQYYLHSRDFYIKI
                     LKDAGLTEKEIDRWEYEFLKSLDERVQLNFFPQFYAIGTKQEI"
BASE COUNT          885 a          650 c          765 g         1052 t
ORIGIN      
        1 gtacctgtat gtttattata gaaacgaaga atcttacaaa aaaattcgac gattttttag
       61 cagttgatca tattcaattt tctgttgaaa aaggagagat ttttgggttt ctcggaccca
      121 atggtgccgg aaagacgaca accattaaaa tgcttacaac cttattatat ccaagtgaag
      181 gatctgcatc catcgcaggg tttgacatcc tgaaacaacg agcgaacgta agagaaaata
      241 taggggtcgt cttccaagaa ccggcattag atacagagct tactggaaaa gagaaccttg
      301 attttcatgc acggatgtac ggattaggac aagaaacaag aaaaaaacga ataatgcaag
      361 tactcaccct tgtcgatttg tcagacaaaa aagatgtttt agtaaagcat tattccggtg
      421 gcatgaaaag acggttagaa atcgctcggg gtttgatgca ttccccgact gtgctttttt
      481 tagatgaacc aacgttgggc cttgatgctc agacgagaag agcaatctgg gagtacatca
      541 agcagttaaa tgaagaagaa ggcacgacga tttttttgac gactcattac atggatgaag
      601 ccgatttttt atgtgatcgt attggaatta tcgatcatgg gaagatactt gtaatggaca
      661 acgtcgatac actaaagaaa ttagttggtc atgatgtgat aacgctatca tcttctgata
      721 gcttagcatt acagaagaga ttggaacagg aaccctgggt gagtaacatt actccacatg
      781 cttctttttt gacggttggt gtcgaacgag gggaagaaaa aatcccgaca atcttcgata
      841 ttgcacggtc aataaatgta tcgatctctt cagttgatgt tcggaaacca acccttgatg
      901 atgtgtttct ttactacact ggacgaagta tgcgggaaca gaacccggag acgcctcgaa
      961 gtagaccatc aaaacctgag aggttccatc tgagacaggg aaaatgatga atcagaagga
     1021 ccttcaagct atctatatgt tatggcttcg gcagatgaag cggtttcttc gtgcaaaaag
     1081 cagggttctc tcaacgattg tgcagccgtt gttcttcctt tttatccttg ggtttgggtt
     1141 taacatcgcg gtatttccag acatgcgagg ctcctatatc aattttctcg ccccggggat
     1201 tatcgcgatg gcgattctgt tctcttctat gtttactggt gtgtcggttc tgtgggatag
     1261 acagtcagga ttcttacaag aggtgcttgt tgcaccggtg agccgactgt cgattgtcat
     1321 cggcaggacc cttggaggtg cgacgatcgc aatgtttcag ggatttatca ttatgattat
     1381 tgctcttgtc ctaggtgtgc ctgttgtgga tgtgcaggga ttgctgctta cggtcgtcat
     1441 catggtgttg ttgtcgttta ccgctgtcgg atttggtttg attcttgctt caaaaatgcg
     1501 agatttcgaa gggttccaat ccattatgag tctgatgctg atgccgttgt tgtttttgtc
     1561 gtctgcgttt tacccgataa atgattcctt accaggatgg ttacaagcgc tttcatattg
     1621 caatccgtta ttttatatga ttgatggtat gcggggcagt ctcacaggtg tcaataatgt
     1681 acttcatccg ttggtagatc ttggcattat cttgtcactg tgcatcatca ttatggggtt
     1741 agggacgtat tttttcaaca agtctgaaat ataatcctca atcaattttt tagaaagtaa
     1801 gggatgattt aatttctttg tagaaatctt gaagtcttaa aagaaatcgt tttatttatt
     1861 ttcaacacag tttttatagg atttcaccat gttagacagt gcacatgcga tcgtcaccgg
     1921 gccgacgcca ccaggaaccg gcgtaagaac agctgctttt tcttttacag attcaaaatc
     1981 gacgtcacca tagatcccat cttcggtttc tatgataccg acatcaacaa caatcgcctt
     2041 gtttttgata tgatccttag tgatgagatg ctttactcct gcgccggtga tcagaatatc
     2101 tgcattggag gtgtacgctt tcagattctt agtaaagata tgagcgatag acacggttgc
     2161 atttctgttc aggagaagtg tcgcgagtgg ttttcccacg atggtactat ggttcacaat
     2221 cacaacgtcc tttccctgga ggtgaacgtg ttcatgctca aggattgtta gaaccgagag
     2281 aggtgtacaa ggaacaagta cctcatcacc gatcatggtt cttccgaggt tttctgggtt
     2341 aaaaccctca acatcttttt tcgctgccac agtctgcatc agtttgtatg gcgagagatg
     2401 cagtggaacc gggtattgga tgaaaatccc atggatcgaa gggtcgttat tgaggctttg
     2461 tatcgtctgg agaatggttg attcggagac gtttgcatcg aatcgcaaaa tctttgatga
     2521 aatgcctacc tcgttgcaag caatggtacg taatttaaga tacaactctg aagagggatt
     2581 ctccccaatg gctatcgtta caaccagggg ttctctctga tatttctttt ttaatcgttg
     2641 cacgtcaaaa gttattttac tacggatttc cttcgcaatc tctcgtccat ttataatcgt
     2701 tgcagtcatt tttttacctc agagatttat gaattgttct tatatttctt gttttgtgcc
     2761 aatcgcatag aactgtggga agaaattgag ttgaactcgt tcatcaagcg atttgagaaa
     2821 ctcatattcc catcgatcga tttctttttc cgtcaaccct gcatccttca atatcttgat
     2881 atagaaatcc cgagagtgaa ggtagtattg tttatcttcc tcgcaagacc agattcggtt
     2941 attcccgata ccgatgacgg tatcaagtcc agcggtaatg aagagtttcc gtaatttccg
     3001 tccgatatgc gggtctccgc ctttttttct gatggcttct ccagcaaaga ggggatctac
     3061 ctttttgttc tctggccagt ggattttccc gccgtaatct ggttcaaggg atgcaaggat
     3121 ttttcctttt ggtttgacaa ccctggtcat ttcgttgaga accatttgcg gatcatctgc
     3181 ccacatcaag agtaaattac aggtgataag gtcaaatgtg tcgtctgcaa aacataattg
     3241 ttgcgcatca cctgtgcaga aggcgacatt ggtatattca ttgagtatct tgtgtgcaaa
     3301 tgtcaaaagg gagagagatc catcaatagc gattactgtt ccttttgtaa gg
//
LOCUS       DATMXJ010000045         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_32317, whole genome shotgun sequence.
ACCESSION   DATMXJ010000045 DATMXJ010000000
VERSION     DATMXJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32317"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..2116
                     /gene="tgtA"
                     /locus_tag="VN377_01335"
     CDS             173..2116
                     /gene="tgtA"
                     /locus_tag="VN377_01335"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HWR27058.1"
                     /translation="MTFEIKERDAAGRLCHFTTKHGGVTTPNLLPVINPNKMMITPRE
                     MKKRFGTDILITNSYIIYKNPELRKKALEIGVHALIDFDGSIMTDSGTFQNYVYGDVH
                     LDPIEIVQFQRDIGSDIGTILDVFGTPGQTKEVSEQGIRETIERAAKSIPHKGEMLLA
                     CPVQGSIYPELREQCARQLSALPADFFPIGGVVPLMENQRYSDLVRCILAAKQGLDPS
                     KPVHLFGAGHPLIFSLAVALGCDFFDSSAYAKYAQDGRMIFQWGTEKFDDLAELPCSC
                     PICSKYSVPELRDLDVEERTMQIALHNLYVSFMELKIIRVALREGWLWDLVEQRAAAN
                     PFLSDALRVLREPAHIQWLEHQEPTSKKTALFYTGPQTMYRPMIFRYLSRLFRRFEPL
                     FHSTVVFPEGTKPYSLFYAKEMANILKQKNVNLLVHSHFGPVPFELDEMYPLAQSVFP
                     TCVDQETKEKAQQAMERFLANTNVIIWDEQMMTQTDKPLAHPIDFDKRRISAVADMQF
                     GPGASEALFAGKIRIVKSKRTGKIRNIICDGAHVVSMRAEDGLFTLKIDGGRRLHKRL
                     KYPLLRVVVTDDAVPFIKEGKSVFAKFVLECDPDLRPFDECLIVDEQDIFLGVGRTLL
                     TRNEMLAFRHGMAVKTRDSLKEG"
     gene            2214..2456
                     /locus_tag="VN377_01340"
     CDS             2214..2456
                     /locus_tag="VN377_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27059.1"
                     /translation="MNKFQEISEEIKNLKAARDFLQKKYDTSEFHKKKEKFPQSAVPP
                     APEDEEIYKLLTAIQQLDKYIKELQDMQFKVLKQEE"
     gene            complement(2478..3368)
                     /locus_tag="VN377_01345"
     CDS             complement(2478..3368)
                     /locus_tag="VN377_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27060.1"
                     /translation="MTKYLITKWFGTFLFDETTVQKEILFPKQTKEIVQRLREIEKNN
                     ILAEEETIAKNTHVIVNERRLQPLGSYNHEEPVFNTIPIKPEDYGFSKELLHEVSLLL
                     TRERVDEQLQSEDLQLIQMVNALDDLVQTANLLSERLSCWALLPTAKKKVKPFEKTLT
                     AVNDEIARLQDQIEKDMETIAPNTSTIIGPLIGARLIALAGGMQRMAMMPASTIQLLG
                     AEKALFRFKKEGGRPPKHGVIFQHPSINCAPKNERGKVARLLGTKISIAMKADVFTKR
                     NISDELQQDIKVRLQEIRKK"
     gene            3506..3826
                     /locus_tag="VN377_01350"
     CDS             3506..3826
                     /locus_tag="VN377_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27061.1"
                     /translation="MKPEKKERIVLTNVIETELDILKRHVLVLQTLKQNEPAGIIKLS
                     ELTKHPQHMVRYSLRILEQEGLIEPSPQGAVTTESASKATPMLKQKLKEMQDTISEIV
                     KELG"
     gene            3852..3924
                     /locus_tag="VN377_01355"
     tRNA            3852..3924
                     /locus_tag="VN377_01355"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3885..3887,aa:Phe,seq:gaa)
     gene            complement(4071..4478)
                     /locus_tag="VN377_01360"
     CDS             complement(4071..4478)
                     /locus_tag="VN377_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27062.1"
                     /translation="MKKQIVIAIIVGIIITLIAGLSIGWSIPRDNSLLTGDHLLNEDS
                     SHLIGSWKGANSSSSFSLTFFADGSLLFSSYHGNFTVNQHKLICNNHGITLTAEFLFI
                     DDYDTLALTNINSSSDSAFGYFGVPYGIILRRV"
BASE COUNT         1371 a          964 c         1020 g         1329 t
ORIGIN      
        1 gtccagttac atggcatcat tgtctgcgat tgccaccctg cgacgggtcc ttgatccgtt
       61 tcagattgcg taaaacaatt gatttgaaca tccacgattg gaatccatac gtttcctatt
      121 tctagcacta aaaccttaag aggatgtttc ttttacgagg ctgtagaaaa aaatgacctt
      181 tgaaatcaag gaacgggatg cggcagggcg tctctgccac ttcacgacaa aacatggggg
      241 cgttaccacc ccaaacctcc ttccggtgat caacccaaat aaaatgatga tcacccctag
      301 ggagatgaag aaacgttttg gaacagatat tctcatcacg aactcgtata ttatttataa
      361 aaatcctgag ttgcggaaga aagccttaga aattggggtg catgctctca ttgattttga
      421 tggatcgatt atgacggact cagggacgtt tcagaactat gtctatgggg atgtgcacct
      481 cgatccgatt gaaatcgtac agtttcagcg ggatatcgga agcgatatcg gaaccatcct
      541 agacgtgttt ggtactcccg gtcaaacaaa agaggtatct gagcagggga ttcgagagac
      601 aatcgaacga gcagcaaaaa gcattccaca caaaggagag atgcttcttg catgcccggt
      661 ccaaggatcc atataccccg agctgcggga acagtgcgcc cgacaactca gtgctctacc
      721 tgcggatttc ttccctatcg gtggcgtcgt ccccctgatg gagaaccagc gatacagcga
      781 tctggtccgt tgtattcttg cagcaaaaca aggtctggat ccctccaaac ctgtgcacct
      841 gtttggtgct ggtcatcccc tgattttttc cctggctgtc gctcttggtt gtgatttctt
      901 tgactcatcc gcgtatgcaa agtacgcaca ggatggacgt atgatttttc aatgggggac
      961 cgagaaattc gatgatctcg ccgagcttcc ctgcagttgt ccgatttgtt cgaaatattc
     1021 tgttccagag ctgagggatc tcgatgtgga agaacgcaca atgcagattg cgttgcataa
     1081 tctgtacgtt agtttcatgg aactgaaaat aattcgagtc gctttaagag aaggatggct
     1141 ttgggattta gtcgagcagc gagccgcggc aaacccgttt ctgtctgatg cactgcgagt
     1201 gttacgagag ccagcgcaca tccaatggct tgaacaccag gaaccaacga gtaaaaaaac
     1261 agcattgttc tatacggggc ctcagacgat gtaccgcccc atgattttcc gctatctttc
     1321 acgattgttc aggagatttg aaccattgtt tcattccacg gtagtgttcc ctgagggaac
     1381 aaaaccgtat tcactgtttt atgcaaagga aatggcaaac atcctcaaac aaaaaaacgt
     1441 caacctgctc gtccattcac attttggccc tgttcccttc gaacttgatg agatgtaccc
     1501 tttagcccag tctgtatttc caacgtgtgt cgaccaagaa accaaggaga aagcacaaca
     1561 agccatggag aggttccttg caaataccaa tgttattatc tgggatgaac agatgatgac
     1621 acagactgac aaacctctcg cacatccgat tgactttgat aaaagacgta tctctgcggt
     1681 tgcagacatg cagtttggac ctggtgcatc cgaagcactt tttgctggaa agatacgcat
     1741 cgtcaaatca aaacgaaccg ggaagatcag aaatatcatc tgcgatggag cgcatgtggt
     1801 ttcaatgcgt gcagaggacg gtttgtttac attaaaaata gatggaggtc gccgactgca
     1861 taagagattg aaatatccct tgttgcgtgt ggtggtgacg gatgatgctg taccgttcat
     1921 aaaagaagga aaaagcgtat tcgcgaagtt cgtcttggaa tgtgaccccg atctgcgacc
     1981 gtttgatgaa tgtttgattg tcgatgagca ggatatattc ttaggggtag gccgaacgct
     2041 tctcacgaga aatgaaatgc tcgcgttccg tcatgggatg gcggtaaaaa caagggattc
     2101 cttaaaagag ggatgatacg atttatccaa aactattatt atctcaatgc ttattaccaa
     2161 ctcagttgca ctcaaaacaa aaaaaaagaa aaatgacgaa aaggaatggg aacatgaaca
     2221 aatttcaaga gatctccgaa gaaataaaga acctcaaagc cgcccgtgat tttttacaaa
     2281 agaaatatga cacctccgaa tttcataaaa agaaggagaa atttccgcaa tcagccgtcc
     2341 cgccagcacc ggaagatgaa gagatctaca aactgctcac tgcgatacaa caacttgata
     2401 aatatatcaa ggaactccag gacatgcagt tcaaagtcct caagcaggaa gaataaaaaa
     2461 gatacgttta gggcttgcta tttttttctt atttcctgta gtcgtacctt gatgtcttgt
     2521 tggagttcat cggagatatt tctcttggtg aagacatctg ctttcatggc aattgatatt
     2581 tttgtcccaa gaagtctggc cacctttccc cgctcgtttt ttggtgcgca gtttatcgat
     2641 ggatgctgga agataacgcc gtgctttggt ggtcgccctc cctctttttt aaagcggaat
     2701 aaagcttttt ctgctcccag gagttgaatg gttgaggcag gcatcatcgc catccgttgc
     2761 atcccaccag cgagagcaat gagtcgtgcg ccgatgagcg gtccgataat ggtactggtg
     2821 tttggggcta ttgtctccat atccttttca atttgatctt gtaatcgagc aatctcgtcg
     2881 ttgaccgcgg tcagagtttt ttcgaacggt tttactttct tttttgcggt tggtaacaat
     2941 gcccagcaac tgaggcgttc agacagaaga tttgcggtct ggacaaggtc gtcgagtgca
     3001 ttgaccattt ggatcagctg taaatcctcg ctttgtaatt gttcatctac tctctctcgc
     3061 gtaagtaaca atgatacctc atgcaagagc tctttggaaa acccatagtc ttcaggttta
     3121 attggtatgg tgttaaaaac aggttcttcg tgattgtaag aaccaagtgg ttgtaaacgc
     3181 ctttcattga cgataacatg tgtatttttt gcaatggttt cttcttcagc aaggatgtta
     3241 tttttttcta tctcgcgtaa acgttgtaca atttcttttg tttgttttgg gaagagaatt
     3301 tctttttgaa ctgtggtttc atcaaagaga aatgttccaa accattttgt aatgaggtac
     3361 ttggtcatcc tgattgggta agaaattctt tctaatgtac tttacgattg acatttctgg
     3421 ttttacccta gtgataaaag agattttaaa tataggaacc tatattacgt gtgttaacaa
     3481 gtatcgaagg attgggggtt ttttaatgaa accggaaaaa aaagaaagga ttgttcttac
     3541 aaatgtcatc gagacagagc tcgatatctt aaaaaggcat gtgcttgttc ttcaaactct
     3601 aaaacaaaat gaacctgctg gtatcattaa attatctgaa ctgactaagc acccgcagca
     3661 tatggtgcga tattcgttac gtatcttaga acaagaagga ctcatcgaac cgtccccaca
     3721 gggcgcagtc accacggagt cagccagtaa agcaactccg atgctgaaac aaaaattaaa
     3781 agaaatgcaa gacaccatca gcgagattgt caaagaacta gggtaaaatc agtgtagaca
     3841 atcttttttt tgccgcggta actcagctgg gagagtgcac ggctgaagac cgtgatgtcg
     3901 agggttcgat cccctcctgc ggcatacctt taatgtccta taaagtgggg attaatagag
     3961 ataaataaca atttctctat tttcttgact tatgtgtttt ctaaagaatt gaagtagcat
     4021 ataaacctat catcgattta gaacaagcat tgaaacggtt taaatataag ttagactctt
     4081 cgcaggataa ttccataagg tacaccaaaa taaccaaagg cagagtcaga gctactgttt
     4141 atattcgtaa gcgctaatgt gtcataatca tcgataaaaa ggaactctgc tgtcaatgtg
     4201 attccatggt tattacagat gagtttgtgt tgattgactg taaaattgcc atgatatgaa
     4261 gaaaacaata aactgccatc tgcgaaaaaa gtcaaagaaa aagaagagct actgtttgct
     4321 cctttccaac ttcctattaa atgagaacta tcttcattta atagatgatc tccagttaat
     4381 aacgaattat ctcttgggat cgaccaccct attgaaagac ccgcaattaa tgtaattata
     4441 atacctacaa taatagcaat cacaatctgt tttttcataa tacctttgcc tccactctac
     4501 tgtttagtaa taattctttc tttaaaaacg tttagccgac cttggctaca tcccgaaatg
     4561 aattaagaat taaagatctg agtagagatt ttatctacat cttgaccctt ttcagagaaa
     4621 aaattgattc aggcatggcc acttggagcc aggcatgaat caatagaacc ggttttttta
     4681 agaa
//
LOCUS       DATMXJ010000046         6777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53264, whole genome shotgun sequence.
ACCESSION   DATMXJ010000046 DATMXJ010000000
VERSION     DATMXJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53264"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VN377_01365"
     CDS             <1..522
                     /locus_tag="VN377_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR27063.1"
                     /translation="ERIEKLENILGQQQKHPRSIEKIFPTQDVPSIDRSDKDVRNEKN
                     LIQNISTEEEIILKERIENHLIIDEMDHIVAVIQRGILREISNSFADFLGYERTELLQ
                     KNFFVFISPSGFEDARKYYLNRLKGMATNSFRTILLTKAQTELPVEITVAPTVYKGES
                     AEFISIKEIKDQS"
     gene            692..3181
                     /locus_tag="VN377_01370"
     CDS             692..3181
                     /locus_tag="VN377_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27064.1"
                     /translation="MKKITILFIVGILISSTIGAIGTQGKQGTTQSLSEHLSTPTIQK
                     QSVDNEGYLLIELTGTSTFQTTAGQPQLPKIVHTIELPFGATDIRVSLTPGGITTQHI
                     DQEIRPATQMVPLTEEYQSLAAYATTKDTQVYAMTTPYPETWFTTSIGVGLNKNNEHV
                     TFVSIHYYPIRYTPATGTLQIASGADLSITYSTPTHNPFPQKSEYNLVIIAPKSFEKA
                     LQPLIEHKNSHGVITVLKTTEDIYTEFTGVDKPEKIKYFIKNAIETWGVQYVLLVGGL
                     KNMVFAKPRDNANEGSTGWHLPVRYTNLYDNPKFPLAAESAIFDPGVISDLYYSDIYE
                     AGGNFSSWDPNGDGTFAAWGKPGVENDTGIDMYPDVALGRLACVNVNEVRTVVKKIIT
                     YETTAYGSEWFKKMTVISGDGFLDQEDLNIQWDTNSLPTGAYTICAQSSNPSAEYGPI
                     ETINITVDKTQETNLTFNHDDNLRIHGYPGLPMAEIVTVSEGNILGNTDFTYTPGENE
                     AYCNWFFFWANMSYLSGVLTIRGKSYDPEPYGNLSSIHVWIKNDADTIVFEDWRNNTE
                     MYYEGEYTTGEKALLGRGGALYYMPEEFQRDIIWASNGLFTGEQDVIDAWTEGAGFMF
                     ISGHGSPNIWADHAPGVPGNRQYGSIDGLLVTTIRPYPPFFTLPLYPMDTIRNEEKLP
                     VTVIGGCHNSQFNVSMIYGLLDGMIYMLPNFPKLHMWCYGTMVPEAFSWRLVRNPHGG
                     SIATMGNTGLGYGMPGIDLTTGGGDGWITIEFFKQYGSEGLDVLGQAYQQTLTSYVNT
                     FDMTDLGAGHPKSVQQWVLLGDPSLKIGGYE"
     gene            complement(3237..3584)
                     /locus_tag="VN377_01375"
     CDS             complement(3237..3584)
                     /locus_tag="VN377_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide isomerase family protein"
                     /protein_id="HWR27065.1"
                     /translation="MKNLEKCTLIETEQDLEKILHTKDTFFILFYAEWCPFSQRFLPI
                     FERCAKDSYHECYRMNIDEYPHLCDRYAVAIYPTIIFFEKGKAMERLDGTHGVGLNER
                     QFQELIKVCHLRK"
     gene            complement(3599..4303)
                     /locus_tag="VN377_01380"
     CDS             complement(3599..4303)
                     /locus_tag="VN377_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWR27066.1"
                     /translation="MTPEKNNVTTGKKYFITTCKNGPYLISGAIPLSQKIIKYDSKWD
                     SCEWQDDKTFPKKDTYALCRCGQSLDKPFCDGTHIKINFDGTETAHPTDYCKNTVTLD
                     GPTLTLIDAKDLCASARFCHRAGGIWNLILTSDKPGDKNIAIEEASDCPSGRLVVCDK
                     TTGKNLEPIFEPSIVIIEDPSIGVHGPIWVRGGIPLESANAVRYATQNRMTLCRCGKS
                     QNKPFCDSSHYPEEKQ"
     gene            4551..5708
                     /locus_tag="VN377_01385"
     CDS             4551..5708
                     /locus_tag="VN377_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27067.1"
                     /translation="MREKKIIPIIVAVIFIGVSCFSGCVGPWAIDTLGWEHVDLEGTV
                     VRVWGQLTISESSDNWNEGFVWDTESHQNWEDYQYRVWADNHAGLGLFSLNIDNLTRT
                     TEYHYRAFGEYLKGKSQYRIGADVTFIPGGPRVITDNASGIGLTEMTLNGNLLHMGGA
                     SSSTVYFLYGTDENALNRQTTPEIRTAVGPFTASLTKLATNTTYYYKAVAENDADTWS
                     GIILTVTPGRPVVISRQPGEIGKNHAILKAELWNTGGTTACNVWFVYSDVSPNQLGQS
                     TPPQIMNATGAFQAYIGNLTASTTYWYRAVGDNGVAQGLGDIIEFSTTSTAEIKTTGE
                     LGKPYKPNKYTLDEDLASRIPARYLQILQKYPILLKLLQQPRIRTFLMGLH"
     gene            complement(5732..6307)
                     /locus_tag="VN377_01390"
     CDS             complement(5732..6307)
                     /locus_tag="VN377_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015211554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWR27068.1"
                     /translation="MSLKDALLFNDRNQWRTWLEKKHKTINEVWLLHYKKQSRKQSLN
                     HIDAVEEALCFGWIDSKLKKIDEERFILKYSPRKPRSVWSKINKEKAEAMIISGKMTQ
                     EGLEKIEEAKKHGLWDTAYTNKVKERIPSDLKQALLKNSKAWFNFQSYANSYRNMYCG
                     WIKAAKTKETRQKRIDEVVKRSRLKKKPGID"
     gene            6463..>6777
                     /locus_tag="VN377_01395"
     CDS             6463..>6777
                     /locus_tag="VN377_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HWR27069.1"
                     /translation="MGKDFSQELIAPCGMDCNVCSGYLAMQHDLKGKGIRMSSCKGCR
                     PRDKKCAFLKKRCACLLNHSIQFCYECADFPCEQLAQLDTRYSTLFHMSFLENLASIK
                     KNG"
BASE COUNT         1891 a         1491 c         1459 g         1936 t
ORIGIN      
        1 gaacgaatag aaaaattaga aaacatactt ggtcagcaac aaaaacatcc ccgctccatt
       61 gaaaaaatat ttccaactca ggatgttcct tcaattgaca gatcggacaa ggatgtacgg
      121 aacgagaaaa atctcataca aaatatctcg acagaagagg aaatcatttt aaaagaacga
      181 atagaaaatc atcttatcat tgatgaaatg gatcatatcg tcgcggtcat acaacgaggg
      241 attttgagag aaatcagtaa ttcattcgct gattttcttg gctatgagcg cactgagctt
      301 ttgcagaaga atttttttgt gtttatctct ccgagtggtt ttgaggacgc aagaaaatac
      361 tatcttaatc gattgaaagg gatggcgacg aactcgttta gaacgatatt acttaccaaa
      421 gcacaaaccg aattacccgt tgaaattacc gtggcgccaa cggtttataa gggcgaatca
      481 gctgaattca ttagtataaa agaaatcaaa gaccaatctt aaaatatttc tttttccgcc
      541 ctttttataa cgaaaaaagc tacgaaatca gaagatatat tctgtattca tagggagaaa
      601 acgattttta ttttcctctt gaaaaggacc caaatattta attactactg aactataata
      661 tacaatacta taataggggg tttaaaataa aatgaagaaa ataacaattc tgtttattgt
      721 tgggattcta ataagctcca ccattggagc tattggaaca caaggcaagc aaggaacaac
      781 acagtctctc tcagagcatc tcagtacacc aacaatccaa aaacaatccg tggacaatga
      841 aggatatctc ctgatcgaac tgaccgggac atcgacgttt cagacaacag caggtcaacc
      901 tcagctaccg aagatcgttc ataccataga gcttcctttc ggggcgaccg atatccgtgt
      961 ttctcttacg cctggtggca tcaccactca gcacattgac caagagatac gacctgctac
     1021 tcagatggtg cctctcaccg aagagtacca aagcttggct gcatatgcta caacgaaaga
     1081 tacacaagtc tatgctatga ccacgccata cccggagaca tggtttacga catcgattgg
     1141 tgtgggtttg aataaaaaca acgaacatgt gacctttgtt tccattcatt attatccgat
     1201 tcgatataca ccagccacag gaacccttca aatcgctagc ggtgcagatc tctccattac
     1261 ctattcaaca ccaacgcaca acccctttcc acaaaaaagc gaatataacc ttgtaatcat
     1321 cgcaccaaaa tccttcgaaa aagcactcca accactcatc gagcataaga atagccatgg
     1381 agtaatcacg gtactaaaaa caacagagga catctacact gaatttacag gtgttgacaa
     1441 accagagaag attaaatatt tcataaaaaa cgctattgag acctggggcg tccaatacgt
     1501 cctgctggtt ggcggactga aaaacatggt ttttgcaaaa ccccgtgata atgcaaacga
     1561 agggagcaca ggatggcatc tgcctgttcg gtacaccaat ctctatgaca acccaaaatt
     1621 cccattagct gcggaaagtg ctatctttga ccccggtgtc atcagcgatt tatactattc
     1681 agatatctac gaggctggag gtaatttctc cagctgggat cccaatggtg atggaacatt
     1741 tgctgcatgg ggaaaaccag gggttgaaaa tgacaccggt atagatatgt atcctgatgt
     1801 cgcactcggt cggcttgcat gtgtcaacgt caatgaagta cgaacggtcg taaaaaagat
     1861 catcacctat gagacaacgg cctatgggtc agaatggttc aagaaaatga ccgttatcag
     1921 tggtgacggt ttccttgacc aagaggattt gaacatacag tgggacacca acagtctccc
     1981 aactggggcg tataccatct gtgcccagag tagcaatcca tctgcagaat acggacctat
     2041 cgagacgatc aacatcactg ttgacaagac acaggagacc aaccttacct tcaaccatga
     2101 tgataacttg cggattcatg ggtaccctgg attgcccatg gctgaaatcg taaccgtgtc
     2161 cgaaggaaat atccttggta acaccgactt tacctacact cccggggaaa acgaagccta
     2221 ttgtaactgg ttctttttct gggctaacat gagctattta tctggcgtgc tcacgatccg
     2281 cgggaaaagc tatgatcctg aaccatacgg aaatctcagc agcatccatg tctggataaa
     2341 aaatgacgca gacactatcg tgtttgaaga ctggcgaaac aacacggaga tgtactacga
     2401 gggtgaatac accaccggtg aaaaagcatt actcggtcga ggcggtgcat tgtattacat
     2461 gcctgaagag tttcagcgag atatcatctg ggcatcaaat ggcttgttta caggcgaaca
     2521 agacgtcatt gacgcctgga ccgaaggagc tggtttcatg ttcatctccg gtcatgggag
     2581 tccgaatatc tgggctgacc atgcccccgg tgtgcctggg aaccgccagt acggcagcat
     2641 cgacggcttg ctcgtcacca cgattcgtcc ttacccacca ttttttacac taccgctcta
     2701 tccgatggat accatacgaa atgaagagaa gctcccggtc acggtgattg gaggatgtca
     2761 taatagtcag ttcaacgtct cgatgatcta tggactcctc gatgggatga tctatatgtt
     2821 gccgaacttc cccaaactcc acatgtggtg ttacgggacc atggtgcctg aggcgttcag
     2881 ctggagattg gtccgaaacc cgcatggcgg ttctatcgcg acgatgggga acaccggact
     2941 tgggtatgga atgcctggta tcgatcttac aaccggcggt ggtgacgggt ggatcaccat
     3001 tgagttcttc aaacagtatg gatcggaagg attagatgtc ctcgggcagg cgtaccaaca
     3061 gacattgaca agctacgtca atacctttga tatgaccgat cttggtgcgg gtcatccaaa
     3121 atctgtccaa cagtgggtat tgctcggaga cccgtcatta aaaatcggtg gatacgaata
     3181 aggccaccac ctttttcctt tttttttaaa aaaaatcctg atatcttttc taatttttat
     3241 ttccgaagat ggcatacctt gattaattcc tggaattgtc tttcgttcaa tccaactcca
     3301 tgggtaccgt cgagtcgttc cattgctttt cctttttcga aaaagataat cgttggatat
     3361 attgcgacgg catatcggtc gcagaggtgc gggtattcat cgatattcat acgataacat
     3421 tcatggtatg aatcctttgc gcatctctca aaaataggta aaaatctctg ggagaaagga
     3481 caccattctg catagaagag gatgaaaaag gtatcttttg tatgaaggat tttctccagg
     3541 tcttgttcgg tttcgataag cgtacatttt tcaagattct tcatccggta ctcacctctt
     3601 attgtttctc ttccgggtaa tgactgctat cacaaaacgg tttgttttgt gattttccgc
     3661 atcggcagag ggtcatgcga ttctgtgtcg cgtatcgtac tgcatttgct gactcgagcg
     3721 ggatgcctcc gcggacccaa atcggaccat ggactccgat ggatggatcc tctattatga
     3781 cgatggacgg ttcgaagata ggttccaggt ttttcccggt ggttttatcg catacaacga
     3841 gtcgaccgga ggggcaatct gatgcttctt cgatggcgat gttcttatct cctggtttat
     3901 ctgatgtgag gatgagattc cagattcctc ctgctcgatg acagaagcgg gcagacgcac
     3961 agaggtcttt tgcgtcgatt aacgtcaatg tcggtccatc aagtgtgact gtgtttttgc
     4021 agtagtcggt gggatgtgct gtttccgtgc cgtcaaaatt tatttttata tgggtcccgt
     4081 cacaaaatgg tttgtcaagc gattggccgc atcgacagag cgcataggtg tctttttttg
     4141 gaaatgtttt atcatcttgc cattcgcagg agtcccattt gctgtcatat ttgattattt
     4201 tttgactcag tggtattgca ccgctgatga ggtagggacc atttttacag gttgtgataa
     4261 agtatttttt ccctgttgtt acattatttt tctccggggt catggttcat ctccatttta
     4321 ttcttagggt ttttcaggtg atgaattcgt ttgtatacga gagttttcga ttccttgata
     4381 tctgtgagtc attgggtatt tatgttcttt gcgtctctgg tatctgaaag gacctgtttt
     4441 ttttaaaatt taacagcgtt gttatcccag aaaatatcga caataaatga aatcaatatc
     4501 agtaatattt ataatcagga tattctatca ccctgtcgag gaattaccac atgagagaaa
     4561 aaaaaattat accaattatt gtcgcggtca tattcatcgg agtatcgtgt ttctccggct
     4621 gtgtcggacc atgggcgatt gatacacttg gatgggaaca tgttgatcta gaaggaactg
     4681 tcgtcagagt ctggggacaa ctgacgatca gtgaatcctc agataactgg aatgaaggat
     4741 tcgtctggga cactgaatcc catcagaatt gggaggatta tcaatatcgt gtatgggcag
     4801 ataaccatgc aggtctagga cttttttcac tcaatatcga taatctgacc cgaaccacag
     4861 aatatcacta tcgcgcattt ggagaatatc tcaaaggaaa gagccaatat agaattggtg
     4921 cagatgttac ttttatccct ggtggcccac gagtgatcac cgataacgcc tcaggcattg
     4981 gtctcactga aatgactctc aacggtaatc tcttgcacat gggtggagcc tcatccagca
     5041 cagtctattt cctctatgga accgatgaaa acgcattaaa tcgacaaacc acccctgaaa
     5101 tcagaaccgc ggttggaccg tttaccgctt cacttacaaa actggccacg aacaccacct
     5161 actattacaa ggcagttgca gaaaacgatg ctgacacttg gtctgggatt attttaacag
     5221 tcacacctgg acggcctgtt gtcatctcca gacaacccgg ggaaataggc aagaaccatg
     5281 cgatactgaa ggcagagctc tggaacacgg gtggaacaac agcttgtaat gtatggtttg
     5341 tgtacagtga tgtcagccct aatcaacttg gtcaatcaac gccaccacaa atcatgaatg
     5401 caaccggtgc gttccaggca tatattggta atcttactgc gagtacaaca tactggtata
     5461 gagccgttgg ggacaatggc gtagcacaag ggctaggaga tatcatcgag ttcagcacaa
     5521 catcaacagc ggaaataaag acaactggag agcttggaaa accgtacaaa ccaaacaaat
     5581 atacactcga tgaagacctc gcttccagaa tccctgctcg ataccttcag attctccaaa
     5641 aatatccaat actgttaaaa ttactacaac aaccacgcat tcggacattc ctgatggggc
     5701 ttcattaaca aagcagacaa cttttttttt tttagtctat ccctggtttt ttcttcagtc
     5761 ttgaacgttt cacgacctca tctattcttt tctgtcttgt ttcttttgtc ttcgcagcct
     5821 taatccatcc acagtacata tttcgatagc tatttgcata agattggaaa ttaaaccatg
     5881 ctttgctgtt ctttaaaagt gcttgcttga gatccgaggg aatgcgctcc ttcaccttat
     5941 ttgtataagc ggtatcccat aatccatgtt tttttgcttc ttcgattttc tctaggccct
     6001 cttgagtcat ctttcctgag atgatcatcg cttctgcttt ttctttgttt attttcgacc
     6061 agacgcttct tggtttcctt ggtgaatatt ttaggatgaa ccgttcctcg tcgatttttt
     6121 taagtttgct gtcaatccag ccgaaacaga gcgcctcttc gactgcgtcg atatgattca
     6181 agctttgctt tcttgactgt ttcttataat gcagcagcca gacttcattt atggtcttat
     6241 gctttttctc caaccaggtg cgccattggt ttctatcgtt gaacaacagg gcgtctttta
     6301 gactcatggt tccgtcttct cccctcgata tatgagcaga tggttatatt tttttatgat
     6361 aggtcggttt gtttttgtct tccttccaac agatactact taatagcctc tactcgctat
     6421 tgcttactat ttcaatttat tacataattc atggtgtgaa cgatggggaa agacttttct
     6481 caggaactta tcgcgccttg cgggatggac tgtaacgtct gcagtggata ccttgcgatg
     6541 cagcatgacc tgaaagggaa aggcatccgt atgtcgagct gtaaaggctg tcgtccccgg
     6601 gataagaaat gtgcgtttct gaaaaaaaga tgtgcgtgtc tcctcaatca ctccatacaa
     6661 ttttgttatg aatgtgcaga ttttccctgt gaacaacttg cgcagcttga tacgagatat
     6721 tcgacgcttt ttcatatgag ctttcttgaa aatctcgcct ctatcaaaaa gaacggg
//
LOCUS       DATMXJ010000047         5003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_68797, whole genome shotgun sequence.
ACCESSION   DATMXJ010000047 DATMXJ010000000
VERSION     DATMXJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68797"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="ftsY"
                     /locus_tag="VN377_01400"
     CDS             <1..816
                     /gene="ftsY"
                     /locus_tag="VN377_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWR27070.1"
                     /translation="DELLWDLEMGLLEADVAYSVIDSIKKDVKQEIRRVPFDKGKAGD
                     LVEMVLKNAIGHVLKSNNFDFNRFIDERKKPVVIMFVGVNGTGKTLSIAKIAYQLQKV
                     GKTCVMAAGDTFRAGAIEQLTIHADNLGVKIVKHGSGSDPAAVAFDAIEHAKAKHKDV
                     VLLDTAGRMQTNSNLMDEMAKIKRVAKPDMIIFVGDALSGNDAVEQAKRFNEVVGIDG
                     VILTKVDADAKGGSSLSIAYTIGKPLLFIGVGQRYEDQITFNPEWMVKHIFGS"
     gene            complement(845..1912)
                     /locus_tag="VN377_01405"
     CDS             complement(845..1912)
                     /locus_tag="VN377_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27071.1"
                     /translation="MISKKYHITTALYELTLRCNMKCIHCGSSAGEQRKEELLTKQWN
                     DLTTQFAEMGCKRIALLGGEPFLRNDWFEISKHIKENNMKVIYVTNGFLINEKTIEKL
                     RYIDPYVVGISIDGASAITHDKIRGLHGSFEKSTNAIQQLKKAGIRTSVITTISTMNF
                     NELPEIRDWIKNKGITWQLQIAIPIGRFQKELMISKENFYAAGLFIAALRRTCSLCEL
                     PIIGAHSFGYFSRVLPNSAILPNWNGCQAGISSVGIQSDGGVKGCLSLPDNYIQGNIK
                     EKSIQEIWNEPDFCSYMRCFNIENLQGNCRNCTYGKKCKGGCLAASIGITGDAFNDPY
                     CFYAIEENFNEMKNTLHKDIV"
     gene            complement(1984..2193)
                     /locus_tag="VN377_01410"
     CDS             complement(1984..2193)
                     /locus_tag="VN377_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27072.1"
                     /translation="MKKEKIYDIGQTLGLSKTEVKAAINRNRNKIFAGLAIGLAFVLR
                     INTGYQPLHYIAPSIKDFDFFMRFF"
     gene            complement(2266..2685)
                     /locus_tag="VN377_01415"
     CDS             complement(2266..2685)
                     /locus_tag="VN377_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR27073.1"
                     /translation="MNSIIRLDTRGRLVIPNEFRETLDLKEGDEVLMSLDTKTNTLII
                     SPIYGKADNLAKIEIQFDDKPGSLAKLAETLAKINIDLVMTESKSYQRGTKARWYIIA
                     DTTKCPYGIDEIRKTLLQNHSVESVSITKIARDRLHR"
     gene            complement(2788..3462)
                     /gene="pyrH"
                     /locus_tag="VN377_01420"
     CDS             complement(2788..3462)
                     /gene="pyrH"
                     /locus_tag="VN377_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWR27074.1"
                     /translation="MKTVVVSIGGSVVLSDEADALFLGKLTTLFKKISKEYKLFVIVG
                     GGKIARRYIQLGRELGFDEATLDLIGIDVTRVNARIITNLLGVSNKEIPHTTSEALKL
                     NQPIVVMGGTDPKHSTDLVGAELAEKTHAVRFVNATNVDGIYDKDPNKYKDAKQLKEV
                     SIDQLIAQYGTKWGAAGKNIFMDEPALEIIQRAKVPTFIVNGKRLDQLEKALMGQAFD
                     GTTILV"
     gene            complement(3513..3944)
                     /locus_tag="VN377_01425"
     CDS             complement(3513..3944)
                     /locus_tag="VN377_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27075.1"
                     /translation="MLIKAIKLKSIEARRYVEPDDKPRQIRIDHNSQISQVINNAEKQ
                     LIVEFQYTSSYGPIGMIKMEGTIVIEDPEAKQLAKEWTDTRKLPDQIASNIHTAVMHT
                     CVPEAVGIAKDLGLPPPIPLPQVRLGSDPKKGQSQSGVEVA"
     gene            complement(4035..4580)
                     /locus_tag="VN377_01430"
     CDS             complement(4035..4580)
                     /locus_tag="VN377_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27076.1"
                     /translation="MSPSVGRTYIPNPLSFFKKDQIDVKLEKYAYTPGETIKGHVGLK
                     LKRPVNAKKLMVALIGLRIVHQGNIAIGPIRVGNQGQQTQVYTIYHFEIPLDEEAVYY
                     HEFYPFEIQIPQDILQSAQQNFNSTLTGLGKTFSEIAQVMNQLSMSGLRVEWSVEAKL
                     QIPLKIDIVNSQKIVLSDQKQ"
     gene            4688..>5003
                     /locus_tag="VN377_01435"
     CDS             4688..>5003
                     /locus_tag="VN377_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWR27077.1"
                     /translation="MKTMKVAMYYNNNDVRIEEMPVPTIGDTELLVQVKASGICGSDV
                     MEWYRIKKAPKVLGHEITGDIVAIGKNVKKYKIGDRVFVSHHVPCNTCRFCKNNQQTL
                     CHT"
BASE COUNT         1248 a         1066 c         1112 g         1577 t
ORIGIN      
        1 gatgagctgc tctgggacct ggagatggga ttgctggaag ctgatgtcgc atattccgtg
       61 attgactcta ttaagaaaga cgtcaaacag gagatcagga gagtcccgtt tgacaaaggt
      121 aaggcaggtg acttggttga gatggttctt aagaacgcga ttggtcatgt gctgaaatcc
      181 aataattttg attttaaccg attcatcgat gagcggaaaa aacctgtcgt catcatgttt
      241 gtcggggtga atgggaccgg caaaaccctt tcgatagcaa agattgctta ccaattacaa
      301 aaagtaggaa agacctgcgt gatggccgcg ggtgacacat tccgtgcagg ggcgattgaa
      361 caactcacga ttcacgcgga taaccttggg gtgaagatcg tgaagcatgg atctggatct
      421 gatcctgccg cagtcgcgtt tgatgccatt gagcatgcga aagcaaaaca taaggatgtg
      481 gtgctgctgg ataccgcagg tcggatgcag acgaactcga acctgatgga cgagatggca
      541 aagatcaaac gggtcgcaaa acctgatatg atcatcttcg tcggcgacgc attgtcgggg
      601 aatgatgcag tagaacaggc gaaacggttc aatgaggtcg tcggtatcga tggagtgatt
      661 cttacgaaag tcgatgcaga tgcaaaagga ggaagctcgc tatcgatcgc gtacacgata
      721 gggaaaccgt tgctcttcat cggggttggt cagagatacg aggaccagat cacgtttaac
      781 cctgaatgga tggttaagca tatctttggg agttagactg cgatttcctc ctattaaatc
      841 aggattaaac aatatcttta tgtaatgtgt tcttcatttc attaaaattt tcttcaattg
      901 cataaaaaca gtatggatcg ttaaaagcat ctcccgtaat acctattgaa gcagcgaggc
      961 agcctccttt acattttttt ccgtatgtac aattcctgca gtttccctgc agattctcaa
     1021 tgttgaagca cctcatatat gaacaaaaat caggttcatt ccagatttct tgaatacttt
     1081 tttcttttat gttaccctga atatagttat ccggaagcga aagacatcct ttcacccctc
     1141 catcgctttg tatcccaacc gaagagattc ccgcttgaca accattccaa tttggtaata
     1201 ttgcagagtt cggcaatacg cgagaaaaat aaccaaagct atgtgcacca ataatcggga
     1261 gttcgcatag actgcatgtt cttcttaatg ccgcgataaa tagtcctgct gcataaaaat
     1321 tttcttttga aatcatcagc tctttttgaa atcgtcctat cggaatagct atctgaagtt
     1381 gccaggttat tcccttattt tttatccaat cccttatctc tggtagttcg ttaaaattca
     1441 tcgtacttat ggttgttatg accgacgttc gaattcctgc tttttttagc tgttggattg
     1501 catttgtact tttttcaaat gaaccatgta atccccttat cttgtcgtgt gttatggcag
     1561 atgccccatc tatacttatt ccaaccacat aagggtcaat gtatcgtaat ttttcaatgg
     1621 ttttttcgtt tattaaaaaa ccgtttgtta catagatgac cttcatgtta ttttccttga
     1681 tatgtttaga gatttcaaac caatcgtttc ttaggaaagg ttctccacct aaaagagcga
     1741 ttcgtttgca acccatttct gcaaactgcg tagtaaggtc attccattgc ttcgtaagta
     1801 attcttcttt tctttgttca ccggctgaag aaccacagtg gatgcatttc atgttgcatc
     1861 gtaaggtgag ttcataaagt gctgttgtta tatgatattt tttcgaaatc atttcttctt
     1921 gctccttgaa tgaaaaaaag ttggagaact gggggataca aaacagttct ccttcttagc
     1981 atcttagaaa aagcgcataa agaaatcaaa atcctttatg cttggcgcta tataatgcag
     2041 tggctgatat cccgtgttta tcctcaaaac aaatgcaagt ccaatggcga gtcctgcaaa
     2101 tattttgttc ctgtttctat ttattgctgc ttttacctct gttttggata gacctagcgt
     2161 ctgtcctata tcatatattt tttctttttt cattaatcac ctcgtttagt gtttccactg
     2221 tagaaaaaaa gtggaccggg aaaatttata aaaaatagcg gccgctcagc gatgaagacg
     2281 atcacgagcg atctttgtga tactcacgga ctccacggaa tggttttgga gtaatgtctt
     2341 tcttatttca tcgataccat aagggcattt tgtcgtatct gcgatgatgt accatcgtgc
     2401 tttggtcccg cgttgatagc tttttgattc ggtcatcacg aggtcgatgt tgatttttgc
     2461 taacgtttca gcgagttttg ccaggctgcc tggtttatca tcgaattgta tctcgatttt
     2521 cgcaaggtta tctgcttttc cgtaaatggg gctgatgatg agggtgttcg ttttcgtatc
     2581 tagactcatg agcacctcgt ctccttcttt gaggtcgagt gtttctcgga attcgtttgg
     2641 gataacgagt cgtcctcgtg tgtctagtcg gattatagag ttcatcgttc acctatgtgg
     2701 gaaatttgtg ggaatacgaa tgcagaaacg atatataaaa ctttctctcc tatcgattcg
     2761 attggaacaa aatagtccta ttccctctta cactaggata gttgtcccat cgaatgcttg
     2821 acccatgagt gctttttcaa gctggtcaag tcgttttcca ttcactataa atgttggaac
     2881 ttttgctcgt tgtatgatct caagagccgg ctcgtccatg aagatattct ttccagcagc
     2941 accccatttg gtcccgtact gtgcgatcaa ctggtcgatc gatacttcct tgagttgttt
     3001 ggcatccttg tatttgttcg gatctttgtc gtagatgccg tcgacgttcg ttgcgttcac
     3061 aaagcggact gcatgggttt tttctgcaag ctccgcacca acaaggtcag tactgtgttt
     3121 cgggtctgtt cctcccatga cgacaatagg ttgatttagt tttaatgctt cgctcgtggt
     3181 atgagggatt tccttattgg agactccaag caggttcgtg atgatccgtg cgttcacccg
     3241 tgtcacatca ataccaatca gatccaaggt ggcctcgtca aagccgagtt cccgtccgag
     3301 ctggatgtac cgtctggcga ttttccctcc accgacgatg acaaacagct tgtattcctt
     3361 acttattttt ttaaaaagag tggtaagttt accaaggaac aacgcgtctg cttcatcaga
     3421 gaggaccact gatcctccaa tggataccac tacggttttc atcagagagc gtatcgtttc
     3481 gatttattta aaagtaccat cggaaaaaat tcttaggcga cttcgacacc gctttggctt
     3541 tgtcctttct tagggtctga tccgagccgt acctgtggta atgggattgg tggtggtaac
     3601 ccaaggtctt ttgcgatgcc aaccgcctca gggacacacg tatgcatcac tgcggtgtgg
     3661 atgttgctgg caatctgatc aggtaatttc ctggtatctg tccattcttt tgccagttgt
     3721 tttgcctcgg ggtcctcaat gacaatggtg ccttccattt tgatcatccc tatcggcccg
     3781 tagctagaag tgtattggaa ctcaacgatg agttgttttt ctgcgttgtt gatgacctgg
     3841 gagatctggc tgttatgatc gatgcgtatc tgccttggtt tatcgtcagg ctccacatag
     3901 cgccttgctt ctattgattt gagtttgatt gccttaatta acataacgtg ttcccaataa
     3961 acgcgcgctc ccttttacgt ctttcgtttc gcaaaaacgt tctttgcttg tgaggttttt
     4021 tcctcaaaaa cgcattactg cttctggtct gataagacga tcttctggga gttcacgata
     4081 tcgattttca ggggaatttg aagctttgct tccacggacc attcgacccg taaccctgac
     4141 atgctcagct ggttcatgac ctgtgcaatc tctgagaatg ttttaccaag accagtgagc
     4201 gttgaattga aattctgctg agccgattgg aggatatcct gaggaatttg tatctcaaag
     4261 gggtagaatt catggtaata gactgcctct tcatccagtg gtatctcaaa atgatagatg
     4321 gtgtacacct gggtctgctg tccctggttg cctacacgga tcggtcctat cgcgatgtta
     4381 ccctgatgga cgatccgcag tccaatgagt gcgaccatga gcttttttgc attcactggt
     4441 cgttttaatt ttaatcctac gtggcctttg atagtctccc ctggcgtgta cgcgtatttt
     4501 tccagtttga cgtcgatttg atccttttta aaaaacgaaa gagggtttgg gatatacgtc
     4561 cgaccgaccg aagggctcat ggaaaagagg atttccagtt ttcatataaa cctatccttt
     4621 cttgcgaatt tttctggctg tcaataaact ctattaaatg cctctttata acaagacgcg
     4681 ggttcttatg aaaacaatga aggtcgcgat gtactacaac aacaatgatg tgcgtatcga
     4741 ggagatgcct gttcctacca ttggggatac cgagcttttg gtccaggtga aagccagcgg
     4801 catctgcggc agcgacgtca tggaatggta ccgtatcaag aaagcaccaa aagtccttgg
     4861 ccatgagatc accggggata tcgttgcgat cggaaaaaac gtcaagaaat acaaaatcgg
     4921 cgatcgagtg ttcgtctccc atcatgtccc ctgtaacacc tgtcgattct gtaaaaacaa
     4981 tcaacagacc ttgtgtcata cct
//
LOCUS       DATMXJ010000048         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53334, whole genome shotgun sequence.
ACCESSION   DATMXJ010000048 DATMXJ010000000
VERSION     DATMXJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53334"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..240
                     /locus_tag="VN377_01440"
     CDS             100..240
                     /locus_tag="VN377_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27078.1"
                     /translation="MNGVPEEWIIFYKTHAFLNKLGYNSDIKDLEKVMFKLNLDDLYM
                     DI"
     gene            596..2518
                     /locus_tag="VN377_01445"
     CDS             596..2518
                     /locus_tag="VN377_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27079.1"
                     /translation="MKKFIPIVIVSILVLSGLGAAAFTTNVSTKQATNIKTESTSVIF
                     SSQPALSEKDGFVEIQLDGATTQLLEQNRPVLPVYVKTYQIPFRSTDIQVVCTVKDIS
                     SLTITQEIIPARIAPLSTLNEQTAYMKDPAVYGSSEFYPSTWYSYDLGAGRNENDQQV
                     TFVKVICYPVRYSPVNNEISLAGGFDVMVTYNAPQTPHQSSAEDYDMVIIAPEKFSSS
                     LQPLIDFKNTKGVITKFKSVESILTEYDGFDAPEQIKYFIKNEYDNSNITYVTLVGAL
                     KSHLYVAKDKDTRSAGWTGWLVPARYVSMPQEEDEGCLSDLYYGCLYNATGFDSWDSN
                     GDGVYAAWNAPGAAKDTFDMYPEVYVSRIPAVTVKEVKAVVKKITTYESTGPADKAWF
                     KNFVGVGGKTFAYYQGKPDGEYLCDLAYNHTKNAFPDLTLTRCYSVNRDTGGRTPVPK
                     DILKSFNEGAGFVDFEGHGNPYSWNTIWFDGTYPEDWTGGFNVYASFLLSNGNKLPII
                     VVGGCHNALYNVSLIPTLLETQARHEHFCYGIPDPTCFSAAFILKTHGGGIGSTGCTG
                     YGFGNGGDPNTLSGALEMNFFYQIGNGTEHLAQAHSLAITKFINENPIDQIGAFCITN
                     WALFGDASLKIGGYSS"
     gene            2645..>3989
                     /locus_tag="VN377_01450"
     CDS             2645..>3989
                     /locus_tag="VN377_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27080.1"
                     /translation="MKKFIPIVIVGILVLSGLGAAAFTTNVSMEHTKNVKTESTSVLF
                     PSQPMLSQKDGFVEVQLDGATTQLLDQNKPVLPIYVKTYQIPFRSTDIQVTCTANDIS
                     TLVLTQEIIPARVAPVSTLSEQVVYVKDPLVYGSSEFYPSTWYSYDLGAGRNENDQQV
                     TFVKVICYPVRYSPVNNEISLAGGFDVTVTYNAPQTPLQSSAEDYDMVIIAPEKFSSS
                     LQPLIDFKNTKGVMTKFKSVESILTEYDGFDAPEQIKYFIKNEYDNSNITYVLLVGGL
                     KSHLFAKDKDTSSAGWKAWWVPARYVNMPQGDDSGCLSDLYYGCLYNATGGFDSWDSN
                     GDGVYAQWIEGQAPKDTFDMYPEVYVARIPAVTVREVKAVVKKITTYESTGPAEKSWY
                     ANFVGVAGKTFEYFEGKPDGEYLCDLSYNYTKNAFPDLQLTTCYTTNRDTGGRTPV"
BASE COUNT         1174 a          794 c          858 g         1163 t
ORIGIN      
        1 atcgttaatg ggggctaaat tatcgacgat gaaaaattga catttgaaga tggtattatt
       61 ttttgtaaac acttatatca atgtgcgatt cataaaacta tgaacggtgt tccagaggaa
      121 tggatcattt tttacaagac acatgcgttc ttgaataaat tgggatataa ttctgatatc
      181 aaggaccttg aaaaggtgat gttcaagctg aatcttgatg atctttacat ggatatctaa
      241 aatctcatct atgaagtcaa tttggttaat gatcagtaaa ccgataggta attttttttt
      301 ttagtcaaga aataatagag gctgttgttt aaaaatcagt aaaataagga aattcatatc
      361 aatagttgat gcgaactggg aggataacag atattccata tcttataatt ccactcgttc
      421 aatgatcgat tctatttcta gctcctaaga gtagaatctt gcctcaatta tacccatttt
      481 tgctgtttat atcgagactt ctataaaaaa aggagttcta ggccatgata ttttattatt
      541 ttggaagagt taaatttaaa tatgatggta tgttaataca ctaatgggga ataatatgaa
      601 gaaattcata ccgattgtaa tagtaagtat tttagtcctg agtggactag gggcagctgc
      661 gttcaccacg aacgtctcaa ccaaacaagc aacaaacata aagactgaat cgacttcagt
      721 tatcttttca tcacaacccg ctctatctga aaaagatggg tttgttgaga ttcaacttga
      781 tggtgcgaca acacaattgt tagaacaaaa tagaccggtg ttacctgtct atgtgaagac
      841 ctatcagatt ccattcaggt caaccgatat tcaagtggtt tgtactgtca aggatattag
      901 ctcattgact ataacccaag aaatcattcc cgcacgtatc gctccattat caaccttgaa
      961 cgagcagact gcatatatga aggatcccgc ggtgtatgga agttccgagt tctatccgag
     1021 tacctggtat agttatgatc ttggcgctgg tcgaaatgag aatgatcagc aggtgacctt
     1081 tgtcaaagta atatgttatc ctgttcgata ttcaccagtg aacaatgaga tttcattggc
     1141 tggcggcttc gatgtcatgg ttacttataa tgcgccacaa acccctcatc agtcttccgc
     1201 tgaggattat gatatggtca ttattgctcc tgagaaattc agctcaagtc tccaaccact
     1261 cattgatttt aagaacacta aaggtgtcat aaccaagttc aaatctgtcg agtccattct
     1321 taccgagtat gatgggttcg atgctcctga gcagataaaa tatttcatta aaaacgagta
     1381 cgataactca aacatcacct atgtgacact ggtcggtgct ctaaagtcgc atctttatgt
     1441 tgcaaaggat aaggataccc gttctgctgg ctggaccgga tggttggtcc cagctcgata
     1501 cgtcagcatg ccacaagagg aagatgaagg ttgtctctct gatctatact acggctgttt
     1561 gtacaatgcg actggttttg acagttggga ttcaaacgga gacggtgtat atgccgcttg
     1621 gaacgcacct ggcgctgcaa aagatacctt cgatatgtat cccgaggtgt atgtctcgcg
     1681 gatcccggcc gtgactgtta aggaagtcaa agcagtcgtc aaaaagatca cgacctatga
     1741 aagcactggt ccagccgata aagcctggtt caagaacttt gttggtgtcg gtggaaaaac
     1801 ctttgcgtat tatcaaggaa aacccgatgg ggagtacctc tgcgaccttg catataatca
     1861 tacaaagaat gcattccctg atttaacact gacccgatgt tactccgtga acagagatac
     1921 aggtggacga acccctgtgc cgaaagatat cctgaaatcg ttcaatgagg gcgctggttt
     1981 tgtcgatttc gaaggtcatg gaaatccgta ctcatggaac acgatctggt ttgatggcac
     2041 ctatcctgaa gattggactg gtggtttcaa cgtctatgcc tcatttttac tctccaatgg
     2101 caataagtta ccgattattg tggttggcgg gtgccacaat gcactctata atgtttcact
     2161 tatcccaacc cttttggaga cccaagcgag acatgagcat ttctgttatg gaattccaga
     2221 cccgacgtgt tttagcgcag cctttatcct caaaacccat ggtggtggaa taggatcaac
     2281 agggtgcact ggatatggtt tcggtaatgg aggcgaccca aatacattaa gcggtgcact
     2341 tgaaatgaac ttcttttatc aaataggcaa cggaacagaa caccttgcac aagctcacag
     2401 tcttgcgata acgaagttca taaacgaaaa ccccatagat caaatcggag ccttctgtat
     2461 caccaactgg gcgcttttcg gcgacgcaag tctaaaaatt ggtggatatt cctcataaac
     2521 gaggatccct ccctcttttt ttttttaatt cgcaacttca taaccttata ttttccaaaa
     2581 agtatatata aacggataga aactatttat ataaggactt gttatcttta attgagggaa
     2641 taatatgaag aaattcatac cgattgtaat agtaggtatt ttagtcctga gtggactagg
     2701 ggcagctgcg ttcaccacga acgtctcaat ggaacatact aagaacgtaa aaactgaatc
     2761 gacatcggtt ctttttccat cacaaccaat gctgtcacag aaggacggat ttgttgaagt
     2821 tcaacttgat ggtgcgacaa ctcaattgtt agatcaaaat aaaccggttt tacctatcta
     2881 tgtgaaaacc tatcagatcc cgtttagatc aaccgatatt caggtaacct gtactgcaaa
     2941 tgatattagt acgttggttt tgacccaaga gatcattcct gcgcgggtcg ctccagtatc
     3001 aactttaagt gagcaggtcg tgtatgtaaa ggatcctttg gtgtatggca gttcagagtt
     3061 ttatccgagt acctggtata gttatgatct tggtgctggt cgaaatgaga atgatcagca
     3121 ggtgaccttt gtcaaagtaa tatgttatcc tgttcgatat tcaccagtga acaatgagat
     3181 ttcattggct ggcggcttcg atgtcacggt tacttataat gcaccacaaa cccctctgca
     3241 gtcttccgct gaggattatg atatggtgat tatcgcccct gagaagttca gctcaagtct
     3301 ccaaccactc attgatttta agaacactaa aggtgtcatg accaagttta aatccgttga
     3361 atctatcctt accgagtacg atgggttcga tgctcctgag cagataaaat atttcattaa
     3421 aaacgagtac gataactcaa acattaccta cgtgttactg gttggtggtc tgaaatcgca
     3481 tcttttcgca aaggataagg acaccagttc tgctggttgg aaagcatggt gggttccagc
     3541 tcgatacgtc aacatgccac aaggtgatga ttcagggtgt ctctctgatc tttactatgg
     3601 ttgtctatat aatgcgaccg gtggatttga cagctgggat tcaaacggtg atggcgtgta
     3661 tgctcaatgg atagaaggtc aagccccaaa agataccttt gatatgtatc cggaggtcta
     3721 tgtggcacgt attcccgccg tgacggttcg tgaagtcaaa gcagtcgtca aaaagatcac
     3781 gacctatgaa agcactggtc cagctgagaa atcctggtac gcgaactttg ttggtgttgc
     3841 tgggaaaacc tttgaatatt ttgaaggtaa acctgatggg gagtacctct gtgacctctc
     3901 atataattat acaaagaatg cattccctga tttacaactc acaacctgtt acaccacaaa
     3961 tagggatacg ggaggacgca ccccagtac
//
LOCUS       DATMXJ010000049        10468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_1024, whole genome shotgun sequence.
ACCESSION   DATMXJ010000049 DATMXJ010000000
VERSION     DATMXJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VN377_01455"
     CDS             <1..348
                     /locus_tag="VN377_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR27081.1"
                     /translation="MVFLGGSKADRDRRASELLAKVGLTNWATHRPSELSGGQQQRIA
                     IARALTNDPSIILADEPTGNLDTKTGEQIMELLVALNREGKTILLVTHAIALKRFANR
                     VVNMLDGEVTEDT"
     gene            345..1655
                     /locus_tag="VN377_01460"
     CDS             345..1655
                     /locus_tag="VN377_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27082.1"
                     /translation="MIRDIFSMALDTMKSYKIRTFLTLIGVIIGVASVIMVTTAGNSV
                     STFIAEQWNLFNPTGMVIGMGTGGTVPQLSIRSVVFTTNDVDNIQRLPSIKTVAPIGI
                     VPVIKILKRDGFLKWESRPGETMYASTASILEILNMELEEGTMFQEGKNEVVISKNVA
                     ETFGKDKRLKIGDTIYLRRVDGKTLDAKVVGILKQSSTLSMFNTLSTPGIICPAKPYY
                     SSYFGSNAGSILKRVVAYTILLAEAKDTSQVQEAQNEILSFLNGTGSDASQYKDQNSD
                     FVVITQQYILDRVDKIMSVIRTYITAIALISLLIGGVGIANIMFATVTERTREIGTMM
                     AIGAKRRDILQLFLYQSMIIGLLGGLAGCILGASGSWFVVDLLNNSIQNLGGAFSTGS
                     IQLSYAPEWFLIASIVGILIGIAAGVLPARKAAKMDPVVALRWE"
     gene            complement(1668..1835)
                     /locus_tag="VN377_01465"
     CDS             complement(1668..1835)
                     /locus_tag="VN377_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27083.1"
                     /translation="MDQKQVLERWLRQLKKNEISPDAVGLGPDKEKALQFIREELEKI
                     EAMEKKQTPSN"
     gene            1954..2136
                     /locus_tag="VN377_01470"
     CDS             1954..2136
                     /locus_tag="VN377_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27084.1"
                     /translation="MSYYWKKNGSLYHWHLQCRAVPVYVRTNLEWEISEQRPNDKERC
                     KECLEKDIALKMKQKM"
     gene            complement(2159..3766)
                     /locus_tag="VN377_01475"
     CDS             complement(2159..3766)
                     /locus_tag="VN377_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HWR27085.1"
                     /translation="MRDQSMITKTGNDLDIILITGEYYDDHPLSPVGVIAKVLDAKGY
                     RVGIIEKPETQEHYTKLGAPRLCFGVTSGSIDSMVHNYTPLKRKRVEDKYSDATTLPD
                     RAVIYYCNKLKQYFKSSVIVIGGIEASLRRFAHYDYWDNNIRRSILLDSRAHILVYGN
                     GEKQILEIAERLQQGKELAGIQGTCILQKDLDSTFELLPSFKEVVEDKRKFCAMQMKF
                     SNDKNLAQEYTNSFVVQYKYPDYTTKDLDWIYSLDYSRKLHPKSLLKMGRFSVVIHRG
                     CIGACNFCSLSLHQGNRIISRSETNILDEIRRLTTHPGFKGYIDDFVGPSSNMYAMVC
                     TRPRSTTACSETCIQCPQLDKTHKRLISLLRKARQIQGVKKIFIRSGIRYDLALESRE
                     YIKEISDHHVSGCLKIAPEHFSNNVLSLMGKDNRRFDEFLSYFTAINQRKKQGLRYYF
                     MIGHPGDTLSEVIYLHDIIKKRHLENIEQFQLFTPTPMTISSCMYWTGYNPFTGRKIN
                     VVYDYHTKKKLKRIMLNLPLKKSTDDD"
     gene            3847..4638
                     /gene="surE"
                     /locus_tag="VN377_01480"
     CDS             3847..4638
                     /gene="surE"
                     /locus_tag="VN377_01480"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWR27086.1"
                     /translation="MTDILVTNDDGYTSAGFVTLVKELSKNYSVAAVVPDCGRSWIGK
                     AITTKKELKIKKITYDGVEMFLLNGTPADCVQIGLYDIVTVRPKMIISGINIGLNIGK
                     ARTLSSGTIGAAIEGSIDGVRSIASSLSIPVTLRDKKTDFYTPKNNPLFENAAKITAK
                     VTTIIMNKAFSDVDLFSLNIPFDATVSTPLEITSLFNTRYGRLFHKTGNKFIHRTPPI
                     QFKDMEEKSDLKALSEGKISLTPMNISFSAAHLLKSVEQTFQGEW"
     gene            complement(4652..5791)
                     /gene="cax"
                     /locus_tag="VN377_01485"
     CDS             complement(4652..5791)
                     /gene="cax"
                     /locus_tag="VN377_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HWR27087.1"
                     /translation="MSPFIVGRRNLVRGFYSPPLKKTIIQKLKIGRTFYVLLAFIPVS
                     ILLYVFTKEDSIIFITSVLAIVPLARIMGYTTKEISLQTNPTVSGLFSATFGNAIELI
                     IAILALNAGLIQVVQASIIGSIIGNILLLVGLSIFAGGIRYKNQRFNNETIAVSSTML
                     IIVVAGLAIPSVYEIVTPKNPQILLLSSAVAIVMALIYLAGLFFSLRTHKDLFDASDE
                     IKATQERPTMSRRLAALILFLTIVVVTFESEILVSTIEHAAQAIGLSQVFIGLVVIAI
                     ITNIAEISTAVHFSVRNKLDVSIEIGLSSAIQIALFVVPILMLVSQVFNFGFTLVFTP
                     FEILAVILAVLIVNYLSADGRCNWLEGAQLISVYSIIAIAFFFVI"
     gene            5872..6783
                     /locus_tag="VN377_01490"
     CDS             5872..6783
                     /locus_tag="VN377_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HWR27088.1"
                     /translation="MEYDVIILRYGELSLKSTYVRKYFETILVRNIKNAFTRENISVV
                     VRTERGRIYLRTTDLAKSIKILPRIFGIVSFSPAMQTTSSLQDMSAMAQHLTKNVLCK
                     QKSFAIRATRVGTHPFTSQQVAIKIGNDIVQATAARVHLNNPDIELFIEIRDKKSFLF
                     TEKIKGAGGLPLGTQGTVLTLVKNPSSLLAAWYLMRRGCHIIFVTTNQSNEKAICSFL
                     SHWYADAETFFIEQKSNDFYQQLSNIVSEKKCDAVITDHTFETPTKALSDLALLKKNS
                     VVPILSPLLTMTQADIEIQCKDRGILL"
     gene            6780..7685
                     /locus_tag="VN377_01495"
     CDS             6780..7685
                     /locus_tag="VN377_01495"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWR27089.1"
                     /translation="MNIVVFGSGAIGSLFGALLAKHNTVVLVGRTPHIASIQQKGLII
                     NGKTRLTRTVPAVESTKEISLAPDLILLTVKSYDTESASRQLRPLLHGQTMVVSLQNG
                     LDNVQKIEQDIEKKHILAGVTTHGALILKPGVVIHTGKGKTILGELDGHYSERLKTLV
                     TVFNQAGIETQMSTDIGREIWGKAIINSSINPLTGFLGCKNGYLLHNPLLEKTVESVC
                     KESSLIASSYGIRISPVDMIEKTKAVIRDTAQNYSSMLQSIQQGKKTEIDSINGVLLR
                     IGSEHKVDTSLNRILFELITSLRPT"
     gene            7822..9717
                     /locus_tag="VN377_01500"
     CDS             7822..9717
                     /locus_tag="VN377_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27090.1"
                     /translation="MYVKRKQKIMIGVSLLVIAGLLVSGTYVYFEYYMTKKVTPPQQT
                     SITIDDRISPLENQGVVLEVLRIRDRDLINKLLKPGKSWENKPTFYFISNMDGLEYIS
                     KDVTQHGRTKEVMFNTWDTMFEENKIMKDVPEEQETSVITLTIMEQVKSGLLGLKTQN
                     IERDSFTVTYDFRTGRWSGNDYFGDPDGYGYYLGETFEIWFNIYQIDYDGDFIPYWTE
                     VNVLGTDPTVDDSKLDPDNDGIPTSWEWKWGYDPFTWDDHNNLDPDLDGLSNIEEYKM
                     AKWFADPYIQDIYYEVDYMGRGGLFDPPHPLFEESKQGLIERLAEHNIKLFIDDGWPN
                     SPPNGGGQVLPHIEKISQDSGMMLQFYDNYFPDERKGIFRYLVIGHGGGFQHPSKNNV
                     YDTTQIAYVSARFKPLQNAFNFVLGGAVPTARGKRVQLGSLILHEMAHSCSIDADNCH
                     FGGIDNISMGTYLFPNKNYVKTWGQYHSVLNYLYTNAPKTFDLSDGENGPPYDQNDWG
                     YMFTGYFQYNANLIEEPYYSAGTGETLVKSEFRVTDYTYDANLTEQFVNYIGDYSPTD
                     PIKVNWSVYKIIDKEKNPNYREIKIFAQPKIITTQQWVLNREADLDANGTLQFYSFDE
                     LMKEKIR"
     gene            9835..>10468
                     /locus_tag="VN377_01505"
     CDS             9835..>10468
                     /locus_tag="VN377_01505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWR27091.1"
                     /translation="MKIFVTRKIPQPGLDALQKEFTLEVNPADRVLSKEEIIEGIKGK
                     DGLLCLLTDPIDKDVINAEPNLKMIASYAVGYDNIDIATATMRGIPVSNTPGVLTETT
                     AELAWALLFSVARRIVEGDTFTRDGSFKGWAPMLMLGQDVSFKTLGVIGTGRIGTAFA
                     LKSKGFHMNVLYTDEQKNEQLERQLGAKKVPLSQLLKESDFISIHVPLTKK"
BASE COUNT         3226 a         2280 c         2231 g         2731 t
ORIGIN      
        1 atggtttttc ttggaggatc caaggcggac cgggaccgcc gtgcctcaga actccttgca
       61 aaagtcggac ttaccaactg ggcgacccat cgcccctctg agctttctgg tggacaacaa
      121 caacgaatag ctatcgcgcg agctctcacg aacgatccct caattattct agctgatgaa
      181 ccaactggaa accttgacac gaagaccggc gagcagatca tggagctcct cgtcgcatta
      241 aatcgagaag ggaaaaccat tttacttgtc acccatgcca ttgccttgaa acgatttgcg
      301 aaccgagtgg tgaacatgct cgacggggaa gtcacggagg atacatgata cgagatatct
      361 tttccatggc tcttgatacc atgaaatcct ataagatacg gacgttcctc acactcatcg
      421 gggtcatcat tggtgttgcc tcggtgatta tggtcacaac cgctggaaac tccgttagta
      481 cctttattgc agaacaatgg aatctcttca atccaaccgg gatggtcatc gggatgggga
      541 ccggtggaac agtaccacaa ctatcgatcc gttccgtcgt cttcacaacc aacgatgtag
      601 ataatatcca gcgtttacca tcgataaaaa cggttgcacc aattggcatc gttcctgtta
      661 tcaaaatctt aaaacgagac gggttcctca aatgggaatc cagacctgga gaaacaatgt
      721 acgcaagtac agcgtcaatc cttgaaattc tgaacatgga attagaagaa ggaaccatgt
      781 tccaagaagg aaaaaacgag gtagtcatca gcaaaaacgt tgctgagaca tttggtaaag
      841 ataaacgatt gaaaatcggt gatacaatct atctgcgtcg agtcgatgga aaaaccctcg
      901 atgcaaaggt cgttgggatt ttaaaacaaa gttcaacttt gagtatgttt aacacccttt
      961 ccacaccagg gattatctgc ccagccaaac cgtattattc aagttatttc gggtcgaatg
     1021 ccggttcgat ccttaaacgt gtggtcgcgt acacgatcct tcttgctgaa gcaaaagaca
     1081 catcacaagt ccaagaagca caaaatgaaa tattaagctt tcttaatggt acaggctctg
     1141 atgcgagtca atataaggat cagaactctg attttgtcgt cattacccaa cagtacatcc
     1201 ttgatcgtgt cgataaaatc atgagtgtca tcagaacgta tatcaccgcg attgcactca
     1261 tctccctgct tatcggtggg gttggcatag caaatatcat gtttgcgacc gttactgaga
     1321 gaaccaggga gattggaacg atgatggcga taggggcgaa acgacgcgat atcctacaat
     1381 tgttcttgta tcaatcgatg atcatcggac tgttaggcgg tcttgctgga tgtatccttg
     1441 gcgccagcgg aagctggttt gtcgttgacc tcttaaacaa tagtatacaa aatctcgggg
     1501 gggcgtttag caccggttct attcaactat cctatgctcc agaatggttc ctgatcgcaa
     1561 gcatcgtcgg aatcctaatc ggtatcgctg caggagtact ccctgcacgg aaagcagcaa
     1621 agatggaccc tgtggtcgct ctccggtggg aataacattc atttttacta attggatgga
     1681 gtctgttttt tctccatcgc ctcaattttt tcaagctctt cacggataaa ctgcaatgct
     1741 ttttccttat cagggccaag accgaccgca tcagggctga tctcgttttt cttcagttgt
     1801 cttaaccatc gctccaacac ttgtttttga tccatggatt ctcacctcat cagggaccat
     1861 atcgcaggtg atgtatatta actcatagat taaatgagta aaaaagttaa atacggaaaa
     1921 agaatatcac aacaatgaaa ggaggaagag agaatgtcgt actattggaa aaaaaacggt
     1981 tctctgtatc attggcatct tcagtgccgg gcagtccctg tttatgtacg aaccaacctt
     2041 gaatgggaaa tcagtgaaca acgaccgaat gataaagaac gctgtaaaga atgcctcgag
     2101 aaggatatcg ctttgaagat gaagcagaag atgtaagaaa gaaaccatcc gtgactgttt
     2161 agtcgtcgtc tgttgatttc ttcagaggca gattcagcat gattctcttc aattttttct
     2221 ttgtatgata atcatagacc acattgatct ttcgtccggt gaaaggattg tatcccgtcc
     2281 aatacatgca agaggagatc gtcatggggg tcggggtgaa cagctggaac tgctcaatgt
     2341 tctccagatg ccgttttttg atgatgtcat gtaagtagat tacttcacta agggtatcac
     2401 cggggtggcc gatcataaaa tagtatcgca atccttgttt tttccgttga tttatcgcag
     2461 taaagtatga taaaaactca tcgaaccgtc tgttatcctt ccccattaaa ctgaggacgt
     2521 tatttgaaaa atgctcaggg gcgatcttca gacaacctga gacatgatgg tcagagatct
     2581 cttttatgta ctctctgctt tccagcgcga gatcgtaacg gatgccgcta cggatgaaaa
     2641 tttttttcac tccttggatc tgtctggctt tacgtaacaa tgaaataagg cgtttatggg
     2701 ttttatccaa ctgcggacac tggatgcagg tttccgaaca tgcggtcgtt gatcgtggac
     2761 gagtacagac cattgcatac atgtttgagg agggaccgac aaaatcatcg atgtaccctt
     2821 tgaatccagg gtgtgttgtc aatcgtctga tttcatcaag gatgtttgtc tcagaccgtg
     2881 aaataattcg attcccctga tgaagcgaaa gtgaacaaaa attacacgca ccgatacatc
     2941 ctctatgaat cactactgag aatctcccca tttttaacaa ggattttgga tggagtttcc
     3001 ttgagtaatc caatgaatag atccagtcca gatccttggt cgtatagtca gggtatttgt
     3061 actgcacgac aaaactgttc gtatattcct gtgcgaggtt tttgtcgttt gaaaatttca
     3121 tctgcattgc acagaatttc cgtttatctt ctacgacttc cttaaaagac ggtagaagtt
     3181 cgaaggttga atcaaggtct ttttgaagga tgcatgttcc ttgtatccca gctaactcct
     3241 tcccttgttg taaccgctca gcgatctcaa gaatctgttt ctccccattc ccataaacaa
     3301 ggatatgtgc ccgtgaatca agcaggatac tccgccgtat gttgttatcc caatagtcat
     3361 aatgtgcaaa tcgtcgcaag gacgcctcaa taccaccaat gacaatcacg ctggatttga
     3421 agtattgctt cagcttgtta caataataga tcactgcacg gtccggtagt gttgtcgcat
     3481 cgctgtattt atcttcaacg cgttttcgtt tcaagggggt gtagttatga accatgctat
     3541 cgatggaacc agaagtgaca ccaaagcaaa gacgaggggc gcctaacttt gtgtagtgct
     3601 cctgtgtctc tggcttctcg atgataccaa cccgatatcc tttcgcatcg agtactttag
     3661 cgataacccc gaccggtgag agcgggtgat catcataata ctccccggtg ataaggatga
     3721 tatccagatc atttcccgtc tttgtaatca tactttggtc tctcatagaa tgctcctatt
     3781 taaatgttcg atatggatca tttccaccgg aaaggttcat taagcccctg tttttttccg
     3841 gtgccgatga ccgacatcct tgtgacaaac gatgatgggt acacatcagc agggtttgta
     3901 acactggtaa aggagctttc aaagaactat tcagtagctg ctgttgtccc tgactgtgga
     3961 cgaagttgga ttgggaaggc gattaccaca aaaaaggaac tgaaaataaa gaaaatcacc
     4021 tatgacggcg tcgaaatgtt cctcctaaac ggaacccctg cagattgcgt gcaaataggg
     4081 ttatacgaca ttgttactgt cagaccaaaa atgattatct caggcataaa tatcgggttg
     4141 aatattggaa aagccaggac gcttagttct gggacaatcg gagcagccat tgaaggatct
     4201 atcgatggag tacgatccat tgcctcatcg ctttctatcc ctgtcacgct ccgtgataaa
     4261 aaaacagatt tttatacacc aaagaacaat ccgctttttg aaaacgcagc caaaatcaca
     4321 gcaaaagtaa caaccatcat tatgaataaa gcattctcgg acgttgatct attttccctg
     4381 aacatccctt ttgatgcgac cgtgtcaact cctctagaaa tcacatccct ttttaacact
     4441 cgatatggga gactgttcca taaaacaggg aacaagttca ttcatcggac gcctccgata
     4501 caattcaagg acatggagga aaaatcagat ctgaaggctt taagcgaagg aaaaatctct
     4561 cttacgccga tgaacatctc tttttcagcc gctcatttac ttaaaagcgt cgaacaaacc
     4621 ttccaaggag aatggtaaat catatccttc ttcatatgac gaaaaagaac gcgatcgcga
     4681 tgatggaata gacactgatc aactgtgctc cttctaacca gttgcatctt ccatccgcag
     4741 aaaggtaatt cacaattaag accgcaagga ttaccgcaag gatttcaaag ggcgtaaata
     4801 caagcgtgaa accaaaattg aatacctggc tcaccagcat caggataggg acgacgaata
     4861 aggctatctg tatggcgcta ctcagtccaa tttcgattga gacgtcaagc ttatttctga
     4921 cggaaaaatg aacagcggtt gatatctctg cgatattcgt aataatcgca atgacgacaa
     4981 gaccgataaa cacctgtgac agaccaatcg cttgtgctgc atgttcaata gtactaacaa
     5041 ggatctcgga ttcaaaggta acgacaacaa tggtaaggaa aaggattaac gcagcgagac
     5101 gacggctcat ggtcggtcgt tcctgggttg cttttatctc atcacttgcg tcaaaaagat
     5161 ctttatgggt ccgtaacgaa aagaaaagcc cagcgagata aatcaatgcc ataacaatag
     5221 caacagcact gctgagaagt aaaatctgag gattcttcgg tgttactatc tcataaacac
     5281 taggaatcgc tagccctgcg acgacgatga tcaacatggt cgaagatacg gcgatggtct
     5341 cgttattgaa ccgctgattc ttgtagcgga taccaccagc aaaaatactg agcccgacca
     5401 ggagtaaaat atttccgata atactcccaa ttatggatgc ttggacgacc tgtattaagc
     5461 cagcgttcaa agcgagaatc gcgataatca actctattgc gttgccaaaa gtagctgaaa
     5521 aaagaccact cacggtcgga tttgtctgca atgaaatttc ctttgtggta taacccatga
     5581 ttcgagcaag aggaacaata gccaacaccg aggtgataaa gataattgag tcttccttgg
     5641 taaacacata taataaaata ctaacaggga tgaacgctaa aagcacataa aatgttcttc
     5701 cgatttttag tttttgaatg atggtttttt tgagaggagg tgaatagaaa cctctaacca
     5761 gatttctacg tcccacaatg aaaggactca tacgactgga tatcgcatca aagtatctaa
     5821 aatgtttcga cagtgaaaag ttaatgtata tcagtctgat gttgaaaaac gatggaatac
     5881 gatgtgatca tcctgcgata tggggagctt tcgctcaaga gcacttatgt gcgaaagtat
     5941 ttcgaaacaa ttcttgtacg gaatataaaa aatgcgttca caagggaaaa catctcggtt
     6001 gtcgtgagaa cggaacgagg aaggatctat cttagaacaa cagacctggc aaagagcatc
     6061 aaaatattgc ctcgaatttt tggcattgtc tcattcagcc cagcgatgca aacaacctca
     6121 tctttgcagg acatgtccgc gatggctcaa caccttacaa aaaatgtttt atgcaagcaa
     6181 aaaagtttcg ccatacgggc aacacgagta gggactcatc cgttcacaag tcagcaggtc
     6241 gcaataaaaa ttggtaatga cattgtgcag gcaaccgccg cacgagtaca tctaaataat
     6301 cctgacatcg agctttttat tgagatacga gacaagaaat catttctgtt tacagaaaaa
     6361 atcaagggag cgggaggtct tccattaggg acccaaggaa cagtacttac cctggtcaag
     6421 aacccctcat cactccttgc agcatggtat ctgatgcgaa gaggatgcca cattattttt
     6481 gtgacgacga accaatccaa tgagaaggct atctgttctt ttctttctca ctggtatgct
     6541 gatgcagaaa cattctttat cgaacaaaaa agcaatgact tctaccagca gttatccaat
     6601 atcgtatctg aaaaaaaatg cgatgcagtg atcaccgatc atacgtttga gacaccaacc
     6661 aaggcattat ccgatctcgc gctcctcaaa aaaaatagtg tcgtaccgat cttaagtccg
     6721 cttctcacca tgactcaagc ggacatagaa atccaatgta aagacagagg aatactccta
     6781 tgaatatcgt tgtttttgga tcaggtgcta ttggatcatt gttcggtgcg ttacttgcga
     6841 aacataacac ggttgtcctc gttgggagaa ctccgcacat cgccagcata caacaaaaag
     6901 ggttaatcat caacggaaaa acgcgtctca ctcggacagt ccccgcggta gaatcaacaa
     6961 aagaaatctc cctagcacca gaccttattc ttcttaccgt aaaatcctat gacacagaga
     7021 gtgcaagcag acagctccgt cctcttctcc atggtcagac catggtcgtc tcccttcaaa
     7081 atgggttaga taatgtccag aaaatagagc aagacatcga aaaaaaacat atccttgcag
     7141 gtgtgacaac ccatggtgct cttattttaa aacctggtgt cgtcatccat acgggaaaag
     7201 gaaagaccat tctcggagaa ctcgatggcc attactccga gagattgaag acgcttgtga
     7261 cggtattcaa tcaggcagga atcgagaccc agatgagcac tgatattgga agggaaatct
     7321 gggggaaagc aatcattaac tcaagcatta atcctttgac cgggtttctg ggttgtaaaa
     7381 acgggtattt gcttcacaat ccgctgttgg agaagaccgt cgagtctgtg tgtaaagaat
     7441 caagcttgat tgcatcttca tacgggatac gcatatctcc agtggatatg atcgaaaaaa
     7501 caaaagcagt catccgggat acagcacaaa actattcctc catgttgcaa agtatccaac
     7561 aggggaaaaa aacggaaatc gactcaatca atggtgtgct cctgaggatt ggttcagaac
     7621 ataaggtgga tacctcatta aacagaattc tctttgaact gatcacctcc ttgcgaccaa
     7681 cctaagaaaa attacaagga aaatatagtg cttctatgaa taataacaag aacgtatttt
     7741 caataccaat aactttatat aaattgtaac ctatattatt aatataaatc caatcccact
     7801 ccataccgga ggcaaaccat catgtacgtt aaacggaaac aaaaaataat gataggagtc
     7861 tcgctacttg ttatcgctgg tttactagtt tctggaacat atgtctattt cgaatattat
     7921 atgaccaaaa aagtgactcc accccaacaa acttcaatca caatagacga ccggattagt
     7981 cccctggaga atcaaggggt tgtccttgaa gtgttacgaa tccgcgaccg cgatcttatt
     8041 aataagttat taaaacctgg gaaatcctgg gaaaataaac caacgttcta tttcatctca
     8101 aacatggacg ggttagaata tatcagcaaa gatgtgacac agcatgggag gacaaaggag
     8161 gtcatgttta atacctggga caccatgttt gaggaaaaca aaatcatgaa agatgtacca
     8221 gaagaacaag aaacatcggt aattactctt acgatcatgg aacaggtaaa atcagggtta
     8281 ttgggactaa aaactcaaaa tatcgaaaga gactctttta ccgtaaccta tgattttcgg
     8341 actggccgat ggtccgggaa tgattatttc ggggatcctg atggatatgg ctattattta
     8401 ggggagactt ttgagatttg gtttaatatt tatcaaatcg attacgacgg tgattttatc
     8461 ccgtattgga cagaagtgaa cgtcctcggg acagacccaa ccgtggatga ctcaaaactc
     8521 gatcctgata acgacgggat cccaacatca tgggaatgga aatggggata cgaccccttt
     8581 acctgggacg atcacaataa cctagaccca gacctcgacg gtctgagcaa tatcgaggaa
     8641 tataaaatgg caaaatggtt tgccgacccc tacatccagg atatctatta tgaagtcgat
     8701 tatatgggtc gcggcggcct ctttgatcca ccacaccctc tttttgaaga atcaaagcag
     8761 ggactcattg aaaggttggc tgaacataat atcaaactct tcattgatga tggttggcca
     8821 aatagcccac caaacggtgg gggacaggtg ttaccacaca tcgagaaaat atcacaagac
     8881 tccggtatga tgttacagtt ctatgataat tattttccag atgaacgaaa agggattttc
     8941 cgatatttgg tcatcggtca tggtggtggc ttccagcacc cctcgaaaaa caatgtctat
     9001 gacacgacac aaatcgctta tgtctcggca cggttcaaac cactccagaa tgcctttaat
     9061 ttcgtcctcg ggggagctgt tccaacagca cgagggaaac gggtgcagct cggatcgctc
     9121 atcttacatg aaatggcaca cagttgtagc atagatgcgg ataattgtca tttcggggga
     9181 atcgataaca tcagcatggg aacctatctc tttccaaata aaaattatgt taaaacatgg
     9241 ggacaatacc atagcgtact caactatcta tatacaaacg ccccaaaaac gtttgacttg
     9301 tctgatggtg aaaacggccc cccctatgat caaaacgact ggggatacat gtttactggc
     9361 tacttccaat ataatgcgaa cctcatcgaa gaaccatact attcagctgg aaccggagaa
     9421 acattagtga aaagtgaatt tagagtcact gattatacct atgatgcgaa cctgaccgaa
     9481 caattcgtaa attatatagg ggattactca ccaaccgacc ctataaaagt gaattggtct
     9541 gtctataaaa taatagataa agagaaaaac ccaaattaca gagagataaa aatattcgct
     9601 caaccaaaga taatcacgac acagcaatgg gtgttgaaca gagaagctga tttagatgcg
     9661 aacggaaccc tgcaatttta ttcgttcgat gagctcatga aagaaaaaat acggtgatcc
     9721 tttggtatct cccacgaaac acctttcttt ttttttaatt gctaatcacc agacgtgttt
     9781 ttagaaatct ctaaataaca atcgtcgtat ctagaaccga gcatctgatc tcctatgaag
     9841 atttttgtaa ccagaaaaat accccaacct ggacttgatg cattacaaaa ggaattcact
     9901 cttgaggtca accccgcgga tcgtgtcttg agtaaagaag aaatcatcga aggcatcaaa
     9961 gggaaagatg gtctcctctg tcttctgaca gatcccatcg acaaagatgt catcaacgca
    10021 gaaccaaatc tgaagatgat tgcctcgtat gcggtcggtt acgacaatat cgatatagct
    10081 actgccacca tgcgagggat accagtgagc aacacccctg gtgtgttaac cgagaccacc
    10141 gcagaactcg cctgggcgtt actattttcg gtcgcccgac gtatcgttga aggagatacg
    10201 tttaccagag acgggagttt caaaggctgg gcacccatgc tcatgcttgg acaggatgtt
    10261 tcatttaaaa cgttaggggt gattggcacc gggcgtatcg gtactgcatt cgctctgaaa
    10321 agtaaaggat ttcacatgaa cgtcctctac accgatgagc agaaaaacga acaactcgaa
    10381 cggcaacttg gggcaaaaaa agttcccctc tcgcaattac tcaaagaatc agatttcata
    10441 tccatccatg tacctctgac gaaaaaaa
//
LOCUS       DATMXJ010000050         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_74264, whole genome shotgun sequence.
ACCESSION   DATMXJ010000050 DATMXJ010000000
VERSION     DATMXJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74264"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VN377_01510"
     CDS             <1..835
                     /locus_tag="VN377_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWR27092.1"
                     /translation="FIRFQHLQLPRNVKDLVAPPAGLKHRLLKQASEQEILEGIQKSL
                     AEMSAAGTSFFCDFREGGFLGIYQIRKALKHFSINSVILSRPLQMTYDKLEVDRLLEN
                     SDGIGLSSISDWEHSEIEKLASHVRKKKKLFALHASEVEREDIDLVLDLKPNVLVHMI
                     AATKADLEKVNDASIPIVICPRAYLFFRLKHDLELMKKTGVTLLLGTDNAMINNPDVL
                     EEVIVLQKTGLFSLEELLTTVTYTPRKALNLDDCIQGRDLSEKYIVLEKDSLKLLYAS
                     T"
     gene            857..1711
                     /locus_tag="VN377_01515"
     CDS             857..1711
                     /locus_tag="VN377_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27093.1"
                     /translation="MMKVENAMSKELIVGYVPGTIREALKILAKHNVSGMPILKKDTK
                     KVAGVLTRSDIFKNPDEEQIALIMSTKPYTVMKDDDLSTAAKLLYENRIHGLPVIDKR
                     KNLVGIISPTDILKALDDSYDDIVDNYFTRNLVPVYQETPITIIMEIINVTSETALPI
                     LNKECELGGIVSEGDLFKLSHIKESVSQTNLGMGGDEDDWTWEGIRDTIRLYYSTTKV
                     DLPPVAVKEIMITNVIKAYKNDPISEIARKMMKNKISHVPVVSPENKLLGMVTDIDLM
                     ACMFKSSK"
     gene            1745..3397
                     /gene="glyS"
                     /locus_tag="VN377_01520"
     CDS             1745..3397
                     /gene="glyS"
                     /locus_tag="VN377_01520"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HWR27094.1"
                     /translation="MPQDIYDKIMMVAKRRGFIYPSFEIYGGIAGFYDYGPLGSQLKN
                     NVENLWRSFYLLKDNCVEISTPTITLYEVLKASGHVEEFTDLTVDCSRCKRSYKVEDT
                     IEKGMSVEQAVKEDQIICPTCKAELHDAHPVNLMFSTTIGVGEGRKAFLRPETAQGIF
                     TNFHLLYQYSRKRLPFGVVQVGRGYRNEISPRQGTLRQREFSMAEAEVFFDPKDKTHP
                     FFDSIKEKELFLFDNDKELRISVADAVKKKIINNQALAYYVYLTQEFLFTAGVDPKKF
                     RFRKHAANELAHYAAECWDAELYSERFGWVECVGIADRSAYDLNAHIESSSVDMYALR
                     SYEQPREVMMKKVVPKMNVLGPRFGGKAGKIKEQLESLDAATTKKITVTVDGESIDIP
                     KECYEIVESKEKQTGEKFVPHVIEPSYGIDRVLYFLLEHNYVEEKRKEEEYTILKLNP
                     IVAPIKTGVFPLISDENLIKIAKILDRDLRKAGINTFYDESGTIGRRYARMDEIGTPF
                     CITVDHDSLNDNQVTVRNRDTTKQERIKIQDIAPYIKGKISL"
     gene            complement(3403..4719)
                     /gene="lat"
                     /locus_tag="VN377_01525"
     CDS             complement(3403..4719)
                     /gene="lat"
                     /locus_tag="VN377_01525"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HWR27095.1"
                     /translation="MTMKPTDVHATLGKYMLADGFDLVLDLKKSKGCRIYDSRSGKYF
                     LDGFSFFATAPLGCNHPAMLTPDFIKKMGELAVNNPTNSDVYTVEMAEFVDSFAKHAM
                     PKHFHHLFFIAGGALAIENGVKTAFDWKIRKNLDKGVGKKGRQIIHFKDAFHGRTGYT
                     LSMTNTFNMNKTKYFTKFNWPRIDNPMVRFPLTDANLKDVQEREKHAVAQIQDAVAQN
                     PDDIAALIIEPIQGEGGDNHFRKEFFQKLRQLCDEHEMMFILDEVQCGVGLTGKMWAY
                     QHFDFEPDILAFGKKTQVCGIMVSERVDEVKDNVFVVPSRLNSTWGGNLVDMVRCQKY
                     LEIIEEEHLVKNAEVQGKRLLQGLKTVEKKYPQKISNARGKGLMCAFDMLTPEKRDEM
                     KDKLYANGLLVLTCGSVTIRFRPPLNITSEEIDEVLAIVEKTVKAF"
     gene            complement(4817..>5592)
                     /locus_tag="VN377_01530"
     CDS             complement(4817..>5592)
                     /locus_tag="VN377_01530"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWR27096.1"
                     /translation="AKWLEDGKEISIPGEQLFENYYIQDIKGTGAFENYPNRNSIPYK
                     EIYGLKHAKTVYRGTFRMTGWCETIRAIVALGWLNEEPPKGFAGKTFGDLTRHLIGAK
                     PKDDLPQATARYLDLKPYAAIIKRLEWLGLFGNEPLPKDKNNPLDYLNVLTLQKMSMG
                     QHERDMVVMHHEFIAEYPSKKEYVTSTLVDYGIPDGDTSVARTVALPAAIAVKMVLNK
                     QITMTGVHIPVIPEFYNPILDELEEIGITFREKTEPLEN"
BASE COUNT         1549 a         1239 c         1303 g         1501 t
ORIGIN      
        1 attcatccgg tttcaacacc ttcaactccc gcgcaatgtc aaagaccttg tcgccccacc
       61 cgctggattg aaacatcgat tattaaaaca agcatcagaa caggaaatcc tggaggggat
      121 acagaagtct cttgccgaga tgtccgccgc aggtacctcg tttttttgtg atttccggga
      181 gggaggattc ctggggattt atcagatacg aaaggcgctg aaacacttta gtatcaattc
      241 agtaatcctc tctcgtcctt tgcagatgac ctacgacaaa ctggaggtgg atcgactctt
      301 ggaaaactcc gacgggattg gtctgagcag catctctgat tgggagcatt ctgaaatcga
      361 aaagctggca tcgcatgtca gaaaaaagaa aaaactgttt gcattgcatg ccagtgaagt
      421 ggaacgtgag gatatcgatc tcgtccttga tctgaaacca aatgtgttag tacatatgat
      481 tgctgcgaca aaggcggacc tagagaaagt caatgatgca tccattccga ttgtgatctg
      541 ccctcgagca tacctgtttt tccgtttgaa acatgacctg gaactgatga aaaagaccgg
      601 tgtgacgctc ctgcttggaa cggataatgc gatgattaac aacccggacg ttcttgagga
      661 agtaatagtg ttacaaaaaa ccggtttatt ctctcttgaa gaattgttga caactgtcac
      721 gtacactcca aggaaagctt taaatttaga tgactgcatt cagggtcgcg acctatcaga
      781 aaaatacatc gttttagaga aagactcttt aaaactatta tatgcttcaa cataggtagg
      841 gacgagaggg acaacaatga tgaaagtaga aaacgcaatg tcaaaagagt taattgtagg
      901 atacgtgcca ggtaccatcc gagaagcatt gaagatactg gcaaaacata atgtctccgg
      961 catgccgatc ctgaagaagg acacaaagaa agtcgccggt gttctgactc gttctgatat
     1021 tttcaaaaac cctgatgagg aacaaatcgc gttgatcatg agtacaaaac catacaccgt
     1081 gatgaaagac gatgacctat ccactgcagc aaaacttcta tatgaaaatc ggatccacgg
     1141 tcttcctgtg attgacaaac ggaagaacct ggttggaatc atcagcccga ccgacatcct
     1201 caaagcgttg gatgattcct atgatgatat cgtggataat tatttcacca gaaaccttgt
     1261 cccggtgtat caagagacgc cgatcacgat catcatggag atcatcaatg tcaccagcga
     1321 aaccgcacta ccaatcctga ataaagaatg cgaacttggc ggtatcgtca gcgaaggtga
     1381 tttattcaaa ctcagtcata taaaagaaag tgtgtcgcag acgaaccttg gcatgggtgg
     1441 cgatgaggat gattggacct gggaaggaat cagggataca atccgcttgt actattcgac
     1501 gacaaaagtc gatctgcccc cggtcgcggt caaagaaatc atgattacga atgtcattaa
     1561 agcatacaag aacgacccga tctcagagat cgctcgcaag atgatgaaaa acaaaatcag
     1621 ccatgttcct gtggtcagcc ctgaaaacaa gctgctggga atggtgaccg atatcgattt
     1681 gatggcatgc atgtttaaat cctccaaatg aattcccttt ttctagtgca ggagagttcc
     1741 cctgatgcct caagatatct acgataaaat catgatggtg gctaaacgca gaggattcat
     1801 ctatccatca tttgaaatct acggtggtat cgcagggttc tatgattacg gaccactggg
     1861 atctcaacta aaaaacaatg tcgagaacct ctggagatca ttttatcttt taaaggataa
     1921 ctgcgttgaa atcagtacac cgacgatcac cttgtatgag gtgctgaaag catctgggca
     1981 tgtggaggaa tttactgatc ttactgttga ctgcagtcgc tgtaaacgat cctacaaggt
     2041 cgaagatacc atcgagaaag gcatgtcagt cgagcaagca gtaaaagaag accagatcat
     2101 ctgccccacc tgcaaagcag aattgcatga cgcccatcca gttaatctga tgttttccac
     2161 gacaatcggt gtcggtgaag gaagaaaagc gttcctacga ccagagactg cgcaagggat
     2221 ttttacgaac ttccatctgc tatatcagta tagtaggaag aggctgccgt ttggggtcgt
     2281 ccaggttggc agaggatatc gcaatgagat ttccccacgc caagggacac ttcgccaacg
     2341 ggaattctcc atggcagaag cagaggtgtt ctttgacccc aaagataaga cccacccatt
     2401 ttttgactca atcaaagaaa aggaattatt cctttttgat aatgataaag aactgaggat
     2461 ctcggtcgct gatgcggtga agaagaaaat catcaacaac caggcgttgg cgtactatgt
     2521 gtatctgacc caggagtttc tcttcaccgc cggggtggat cccaagaagt tccggttccg
     2581 gaaacatgcg gcaaatgagc ttgcacacta tgcagccgaa tgctgggatg cagagctgta
     2641 cagcgaacgg ttcgggtggg tggaatgcgt cgggatcgct gaccggtccg cgtacgacct
     2701 caacgctcat atcgaatcct caagcgtgga catgtacgca ttacggtcgt atgaacaacc
     2761 acgagaagtg atgatgaaaa aagtggttcc taagatgaac gtgctcggtc ctcggtttgg
     2821 tgggaaagca ggaaagatca aggaacaact cgaatcgctc gatgcagcaa cgaccaagaa
     2881 aatcacagtc actgtcgatg gagaatccat cgatattcca aaagagtgct acgagatcgt
     2941 tgagtcaaaa gaaaaacaga ctggcgagaa attcgtcccc catgtcatcg aaccctcgta
     3001 tgggattgat cgggtcctgt atttcctcct tgaacataac tatgtcgagg agaagagaaa
     3061 ggaagaggaa tacacgatat tgaaactaaa tcccatagtc gcacccatta aaactggggt
     3121 ctttccattg atcagcgatg agaacctcat caagatagct aaaatacttg acagggattt
     3181 gagaaaagca ggcatcaaca ccttctatga cgaaagcggg acgatcggtc gacggtacgc
     3241 acgcatggat gagataggca caccgttttg catcacggtt gatcacgact cgttgaacga
     3301 taaccaggtc actgtcagaa accgtgatac aaccaaacaa gagagaataa aaatacagga
     3361 tatcgccccg tacataaaag gaaagatatc cctgtgatta ttttaaaatg cttttacggt
     3421 tttttctacg attgctaaca cctcatcgat ttcttccgat gtgatgttca atggcggtcg
     3481 gaatcgtatg gtgacagacc cgcaggtcag aacaagaaga ccattggcgt acagcttatc
     3541 tttcatttcg tctctttttt ccggggttag catatcaaag gcgcacatta gacctttccc
     3601 gcgggcattg gaaatcttct gtggatattt cttctcgact gttttcaatc cctgcaataa
     3661 gcgtttccct tgtacctctg cgtttttcac caggtgttcc tcctcgatga tctcaaggta
     3721 tttctggcag cggaccatgt cgacaaggtt cccaccccaa gttgagttca atctgctggg
     3781 aacaacaaac acgttatctt ttacctcatc cacgcgttca ctgaccatga taccacaaac
     3841 ctgcgtcttc ttcccaaatg caaggatatc tggttcgaaa tcaaaatgtt ggtacgccca
     3901 catcttcccg gtcaacccga caccgcattg tacttcatcg aggataaaca tcatctcatg
     3961 ttcatcacag agctgccgaa gtttctggaa gaattccttt cggaagtggt tatccccgcc
     4021 ttcgccttgg atcggttcta tgataagagc agcgatatcg tcagggttct gtgccaccgc
     4081 atcctgtatc tgagcgaccg cgtgtttttc tcgctcctga acatctttga gatttgcatc
     4141 ggtgagcgga aatcggacca ttgggttgtc gattcgtggc cagttgaatt tcgtgaaata
     4201 ttttgttttg ttcatgttaa aggtattggt catggagagg gtataaccgg ttctcccgtg
     4261 gaatgcgtct ttgaaatgga tgatttgtct gcctttcttt ccaactcctt tgtccaagtt
     4321 tttccgtatt ttccagtcaa acgcggtttt gactccgttt tctatagcaa gtgctcctcc
     4381 ggcgatgaaa aagaggtgat gaaagtgttt tggcatcgcg tgtttagcga agctgtcgac
     4441 gaactcagcc atctccacgg tgtacacatc agagttcgtt ggattgttga ccgccagttc
     4501 ccccattttt tttataaaat ctggtgtaag cattgcagga tggttgcatc cgaggggtgc
     4561 ggttgcgaaa aacgagaaac cgtcaaggaa atattttccg gatcgggagt cgtagatcct
     4621 gcatcctttg ctttttttaa ggtctaagac aaggtcgaat ccgtctgcaa gcatgtattt
     4681 cccaagtgta gcatgaacat cggttggttt catcgtcata ttatctatca ctcctttaaa
     4741 ataggattgt tctctgtttg agcggtatat ttccttgtta taaaatgata tcggtcgtct
     4801 ggtgatttag agaatatcaa ttttccaatg gttctgtttt ctccctgaat gtgatgccta
     4861 tttcttcgag ctcatccagg atcggattat agaactccgg gatgacaggg atatgaacac
     4921 cggtcatggt tatctgtttg ttcagaacca tctttaccgc aatagcagca gggagtgcga
     4981 cagtacgggc gacagaggtg tctccatcag ggataccgta atcaaccaac gttgaagtga
     5041 catactcctt tttcgatgga tattctgcga tgaactcatg gtgcatgacg accatgtctc
     5101 gctcatgctg tcccatcgac atcttctgca aggtcagaac attaagataa tctaagggat
     5161 tgtttttatc ctttggcagt ggttcatttc caaacaagcc gagccattca agtcgtttga
     5221 tgatcgcggc atagggtttg aggtcaagat atcttgctgt tgcctgtggg agatcgtctt
     5281 ttggtttcgc accgatgaga tgtcttgtca gatctccgaa tgttttccca gcaaagcctt
     5341 tgggtggttc ttcatttaac caaccaagtg caacgatcgc tctgatggtc tcgcaccatc
     5401 cggtcatcct gaaggtcccg cggtatacgg tttttgcatg tttcaagccg tatatttctt
     5461 tgtatggtat ggaattgcgg ttcggatagt tttcaaatgc tccagtgcct ttgatatcct
     5521 gtatatagta gttttcaaac agttgttcgc caggaataga gatctctttg ccgtcctcaa
     5581 gccattttgc tg
//
LOCUS       DATMXJ010000051         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_74269, whole genome shotgun sequence.
ACCESSION   DATMXJ010000051 DATMXJ010000000
VERSION     DATMXJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74269"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(689..2359)
                     /locus_tag="VN377_01535"
     CDS             complement(689..2359)
                     /locus_tag="VN377_01535"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27097.1"
                     /translation="MKTKLIGFCVMFLVCASTVPLFAESGTSNGNNAITSNTPSGEIE
                     VLSNDLLRYIENKESNNGRLDDPTPGYYDTSEYLIGSVGIGLIFLESNGAIDPSTENW
                     NTTETNNVQSQITQAISWWINQNQWASLSYVLDVHTAVPTSYEPIIHPSAMTDPTYEK
                     LWVSEAMASLGYSSGDWMKRTRDYVNAMRTAKGTDWGLTVFLVDSSNDVGGDFSDGYF
                     AYAYMGGPYIVMTYDNDGWGISAMNQVMAHETGHIFWATDEYDGYTEFSGYLNAADVE
                     GSGCLMDTNFLSLSAGTRLQVGWRDTDLDNILDIIDTNPDTILNPYPQDPTPLQILTY
                     TGVATEVPYQNNNPQPGCAGNDVSINNIMVVFYRIDGGPWNPANPGDGNYDEAMETYT
                     FTTIPIPHGTHLIETFAMNNVGNVDPTLASDTITIDNPPNIPTLTGTVQGKTGQQYMY
                     NGSTVDVDGDPVYYWFDWDDGTNTGWLGPLPPGATMNVNHSWSTKGTYEVKVKAKDIY
                     GMESSWATLDVTMPKNAVFDGNFFRAFLQAHPFLSSLLSFLFERFSFV"
     gene            2746..3642
                     /locus_tag="VN377_01540"
     CDS             2746..3642
                     /locus_tag="VN377_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWR27098.1"
                     /translation="MHKIPEDPHTFMRTIFAGGLERTYRIHVPSNLSENSTPALLFIL
                     HGGGGTGEGMERSLTLGGFNTLADQHNAIMVYPDGFEKNWNDGRKNVSDPAHQQNIDD
                     VGFFTILIENLSQEFTIDPHRIFVTGISNGAMMSYRLAIEISEKIAAIAPVAGAMPID
                     LLSYNISDVPVSLCVLSGTQDPLVPWEGGLVGFPRNPRGIVLSVPESVSFWVAYNNCS
                     TPPNSTLLPDIDPTDHTWVQRDIYSNGFNDTEVILYTIYNGGHTWPDGYQYLPESLIG
                     RTTHDINANMVIWDFFLNHPKE"
     gene            complement(3691..5133)
                     /locus_tag="VN377_01545"
     CDS             complement(3691..5133)
                     /locus_tag="VN377_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWR27099.1"
                     /translation="MKNDPVIVKILRQEERSGKLQNRTSTTTSGTALTNPIVGQKEYV
                     PTNAKETSRYEPKRTIIDLVSPQEKYETIFDNYTVAITLADNEERIVSWNKYTEELLN
                     MQETDLFMKPVHSLYPPEEWEKIRKENVRQKGIRYRMETKMLRKNEGSFDVDLSICIL
                     KGFKGEIVGSIAIITDITELKKVEKELRESEKKYRTIFDNSAVAITMTDENEKIISWN
                     KYAETLLGMNKDDLFQKPVQDLYPVSEWKKIRSQNIRQKGMQHHLETKIQKKNNEQID
                     VDISLSVLKNHEDRVVGSIGVMKDISDRKRTEKNLEESEKKFKLLYEKAPVPYHTLSP
                     DGIITDVNEKWCQVFGYTKDEVCQKSIFDFISEHEKVPAQKSFAQKISRGKSYTGGHE
                     REYLTKTGEKKLFVAHDFFLFDEKGKVISVYTTIEDITEHKQAEQALKEKISELERYK
                     NITVNREMKMMDLKNEINELHAQLHQKPKY"
BASE COUNT         1428 a         1113 c         1029 g         1711 t
ORIGIN      
        1 attatgttgt gctgtagtgg atttaaccct atcatataat tattatttat ctgagtccga
       61 gatttttgtg aatactgaat caaatgttga atttgtgaat ttcctgacag aaggagtctt
      121 tccagctcat actttcataa aattcatcat cgcattacca ctcttattat ttatttttag
      181 ttggtttgat gtattacacg ataacattag gggtaatacc atgttttata tcgagcgttt
      241 tgggagaaca ttcactgtaa caatactgag cttgttttgt gtatcgtact gcggctcagg
      301 attcacatgg tacacgaatt cacaatttct ttatgatttc ctctcagtcg ttgaatcaat
      361 gatccatggt tccatcatga ttgtattatg ttctcttttt ttgttaactc ttatcctttt
      421 agtgacttct ttctctgtta tgcgctctac aaaaatagtg taaatcttct ttgattattt
      481 aaatcaaaaa aacatgagtc ttataatcgt ataaacattc ctcgtataac ggtatagaat
      541 gagatgctat tcttcaaatt catcagtgac tttcatacgg tcttgaggct atcaaagtag
      601 cagctcatga gatgttcaac cagttctgga aagaattcct tttgaaatta aggaatcaat
      661 acctcaactg aagaggattc ctatcaattc atacgaaaga aaatcgttcg aaaagaaaag
      721 aaagtaaaga agagaggaac ggatgtgcct gaagaaaagc cctgaagaaa ttcccatcga
      781 acaccgcatt ttttggcatc gtgacatcaa gggtcgccca agagctctcc atcccataga
      841 tgtcttttgc tttcactttc acttcgtacg tcccttttgt tgaccacgaa tgattcacat
      901 tcatggttgc accaggtggt aaaggcccta accatcctgt attggtccca tcgtcccagt
      961 cgaaccagta atacaccgga tccccatcga catcaacagt tgagccatta tacatatact
     1021 gctgccctgt ttttccttga acagtgccgg taagagtcgg tatattgggt ggattatcaa
     1081 tcgttatggt atcggaagct agcgtgggat caacattccc gacattattc attgcaaacg
     1141 tctcaatgag atgtgtacca tgtgggatcg gaatcgttgt gaatgtgtag gtttccattg
     1201 cttcatcata atttccatca ccagggttcg ccggattcca gggtcctccg tcgatccgat
     1261 agaaaacaac catgatattg ttgatggaaa catcgttccc agcacatcct ggctgaggat
     1321 tattattctg ataaggaact tcagtcgcaa cacctgtata tgtgagtatc tgaagtggtg
     1381 ttggatcctg aggataggga tttaagatgg tatcagggtt cgtgtcgatg atgtccagta
     1441 tattgtccaa atcggtatcg cgccaaccaa cctgtaatcg agtcccagcg gaaaggctaa
     1501 ggaaattggt gtccatcaag caacctgatc cttccacatc agcagcattc aggtacccgc
     1561 tgaattcagt gtagccatca tactcgtctg tcgcccagaa aatatgaccg gtctcatgag
     1621 ccatcacctg attcatcgct gaaattcccc acccatcatt atcataggtc atcacgatat
     1681 aaggaccgcc catgtacgca taagcaaagt aaccatcaga gaaatctcca ccgacgtcat
     1741 tgctgctatc aacgagaaac acggtcaaac cccaatcagt cccttttgcg gttcgcatcg
     1801 cgttgacata atcccgtgtt cgtttcatcc aatcacctga gctataccca agagaggcca
     1861 tggcttcact tacccagagc ttctcatagg tagggtctgt cattgcagag ggatgaatga
     1921 ttggttcgta gcttgttgga acagcagtgt gaacatccag gacatagctc aggctcgccc
     1981 attggttttg atttatccac catgatatcg cttgagtaat ctgactttgt acattgtttg
     2041 tttctgtggt gttccaattt tctgtgcttg ggtctattgc tccgttgctt tcaaggaaaa
     2101 tcaagccgat tccaaccgat ccaatgaggt attcacttgt gtcataatac ccaggggtag
     2161 gatcatcaag tcgaccattg ttcgattcct tgttttcaat ataacgaaga agatcattgc
     2221 ttaggacttc tatttctcca gaaggagtat ttgaggtaat cgcattgttc ccattgcttg
     2281 ttccgctctc tgcaaaaagg ggtaccgtac ttgcacagac taaaaacatc acacaaaaac
     2341 cgataagttt cgttttcata gattcctcca aataagataa ttccattatg atatttatac
     2401 gtttctaatc gagaaattgc aaaataatga tgataaaaat ggttaggttg agtttttttt
     2461 caatcatgaa atacataccc agccccaaag aatcttaaat aatccaatgc catatttcca
     2521 ctaaaaaaaa ctgttgcagg cagtttttta agaaaacatc atttgacaat aatactgttt
     2581 ataagggaaa acaagggaca aattgggtaa aaaattaaat agtgattctt agttaaatta
     2641 aagaaaggaa acatgtgaaa tcttatgaaa aacaacaaag tgaaaataat tcctatatta
     2701 ctcattggaa tcctcattgt cttgcccgta tttctgactg gatgtatgca taaaattcca
     2761 gaagaccctc atacctttat gagaactatc tttgctggtg gactcgaacg gacctatcga
     2821 atccatgtac catcgaacct atcagagaat agcacaccag cgttgctttt tatccttcat
     2881 gggggcgggg gtaccggtga gggtatggaa agaagcttga cccttggtgg attcaatacc
     2941 ttagcagatc aacacaatgc tattatggtg tacccagatg gattcgagaa aaactggaat
     3001 gacgggcgaa aaaacgtcag tgacccagcg catcagcaga acattgatga tgtcggtttc
     3061 ttcaccatcc ttatagagaa tctttcccag gaattcacca tagaccctca tcgaattttt
     3121 gtcacgggaa tatcgaatgg agcgatgatg tcctatcgac tcgctatcga gatatctgaa
     3181 aaaatcgcag caatcgctcc ggtcgcaggt gctatgccaa tcgatttgct ctcctataac
     3241 atctcagatg tgcccgtctc tttgtgtgtc cttagtggca cacaggaccc cctcgtacct
     3301 tgggagggag gcttagttgg tttccccagg aatcctcgtg gaatcgttct ttcggtacct
     3361 gaatcagtat cgttctgggt ggcgtataat aattgctcga cacccccaaa cagtacattg
     3421 ctaccagaca ttgatccaac ggatcacacc tgggtccagc gagatatcta ttctaacggt
     3481 tttaatgata cagaggtgat tctctacacg atctataacg gtggccatac ctggccagat
     3541 ggataccaat atctccctga gtcacttatt ggaaggacga cgcatgatat caatgcgaat
     3601 atggtaatat gggatttctt cttaaatcat ccaaaagaat gagggattga aacagatagg
     3661 gtactctttt gcttgttgtt cctgctgctg ttaatatttt ggtttctgat ggagttgcgc
     3721 gtggagttca tttatctcat tttttaggtc catcattttc atctctcgat tcaccgtgat
     3781 gtttttatac cgctcaagtt cgctgatctt ttcttttaat gcttgttctg cttgtttatg
     3841 ctcggtgatg tcctctatcg tcgtatacac agagatgact tttccttttt catcgaacag
     3901 gaagaaatca tgggcgacga agagtttctt ctccccggtt tttgtaaggt attctcgttc
     3961 atgaccaccg gtatatgatt ttcccctaga gattttctgc gcgaaggact tttgagctgg
     4021 cactttttcg tgctctgaga tgaaatcgaa tattgatttc tgacagactt catcttttgt
     4081 atacccaaat acctggcacc atttctcatt gacatcagtg ataataccat cgggagataa
     4141 ggtatgatag gggaccggtg ctttttcata gaggagttta aatttcttct cagattcttc
     4201 aaggtttttt tccgtacgtt ttcggtcact gatatctttc atgacaccga tggacccgac
     4261 gaccctgtct tcatggtttt tcaggacact taaggagata tcaacatcga tctgttcatt
     4321 gttctttttt tgtatttttg tttcaagatg atgctgcatc cctttctgtc ggatattttg
     4381 tgaccggatt tttttccatt cagagacggg gtataaatcc tggacaggct tttggaataa
     4441 atcatccttg ttcatcccca gtaacgtttc cgcatattta ttccaggaga tgattttctc
     4501 attctcatca gtcatggtaa tggcgacagc agagttatca aaaatcgttc tgtatttttt
     4561 ttctgattct cttaattctt tttccacttt ttttaactcg gtgatatctg taatgatagc
     4621 aatcgaaccg actatttctc ctttaaatcc ttttaaaatg cagatggaga gatccacatc
     4681 aaaggatcct tcatttttcc gaagcatctt cgtttccatt cgatatcgga tgcctttctg
     4741 tcgaacgttt tctttgcgta ttttttccca ttcctcaggt ggatacaagg agtgtaccgg
     4801 tttcatgaat aagtctgttt cctgcatgtt gaggagttct tcggtgtatt tattccatga
     4861 gacaatgcgt tcttcattat ccgctaaggt gatagcaacc gtatagttat caaaaatggt
     4921 ctcatatttt tcctgagggc ttaccaggtc tataatcgtc ctttttggtt cgtaacgaga
     4981 tgtttccttt gcgtttgtcg gcacatattc tttttgtccg acgataggat tcgtaagagc
     5041 ggtcccactc gttgtggtcg atgtacgatt ctgaagtttc cccgagcgtt cttcttgccg
     5101 cagaattttc actattacag gatcattttt cataaaacca tctcctacga tactagatac
     5161 tataaagaaa gattctcaaa gcttttaaag aaaatatatc tatttttccc aatgtcattt
     5221 gtttttttac ttcaccacat cccatcaaaa catgaaatac tttctttata taaaagagta
     5281 c
//
LOCUS       DATMXJ010000052         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_63858, whole genome shotgun sequence.
ACCESSION   DATMXJ010000052 DATMXJ010000000
VERSION     DATMXJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63858"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..613
                     /locus_tag="VN377_01550"
     CDS             86..613
                     /locus_tag="VN377_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWR27100.1"
                     /translation="MTKSSKDLIDQDEKKIISELVKNSKENIDTIAKHCGFSAQKVRR
                     VIKQMEQNHVIWGYTSVFDEKRIGLKHFMMMAKRTPGQVEEKTVDKIIQRELEHHAAE
                     IGITIESSAYVHGDYDWVITFTAEDIVQAKMLSNSLNLLHPNKIEKVSIIQSLMFIKK
                     QYILNPDREILKDFL"
     gene            975..1316
                     /locus_tag="VN377_01555"
     CDS             975..1316
                     /locus_tag="VN377_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27101.1"
                     /translation="MRKVVIFTMLCLLLLIMVPMTVGVSIHRKSEGISAFPDWTFLQG
                     IVTRHRHVHINGGEYVEFNAIFLHYRTHWFGNIRSGFFHHFEKIVLTAFHYGYLGSHF
                     VLAKFSMGLIP"
     gene            complement(1495..1986)
                     /locus_tag="VN377_01560"
     CDS             complement(1495..1986)
                     /locus_tag="VN377_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27102.1"
                     /translation="MTNIHPIQIRNNIEAGDIDDIIFLHGSLYTQEYGFDKTFEQYVA
                     EPLRKFALTQTAREKIWIVEQSNNVRGCLAVVKSDETTAQLRWFLVHPDLQRRGIGTK
                     LIQGAIQFTKENGYRLLFLWTVSILEAANKLYTSTGFRLTEEKARTLWGKPLVEQRFE
                     LVL"
     gene            complement(1983..2480)
                     /locus_tag="VN377_01565"
     CDS             complement(1983..2480)
                     /locus_tag="VN377_01565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27103.1"
                     /translation="MTITIRAASEEDLQAIVEIFNQAVRTRTSTGYLSETTVEERTIW
                     FTEHPPEKYPILIAEQNQRIVGWASIDPYRRGRKAFEKTVEVSLFIRQEYTRKGFGNQ
                     LLIDMMQTAKKLGYTILFAIVLDKNAGSRRLLEKNNFEQWGFLPDVASIDGKILSHVY
                     YGKRL"
     gene            complement(2775..3770)
                     /locus_tag="VN377_01570"
     CDS             complement(2775..3770)
                     /locus_tag="VN377_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWR27104.1"
                     /translation="MDLEEILKERASIFDRALTGNLKPREPEIFYDALRWIPLSGGKR
                     LRPILAMLACEAVGGVPETTIPFGIALEYMHNSTLIHDDIIDKDKWRRGLQTTHEKFG
                     VPFAILAGDALIGETYRMLSYMAPPELNSVTYKEIIRSIADAAKDFYEGEALDIEFAV
                     RLDVTIPQYMRMIEKKTGQLYYLAGKGGALIGKGSNEQVENLAEYGTLFGLMFQIKDD
                     LLNILTEQSVLGKQMIGSDILNGKRTLMFVHALSEVDEKDKKKMLKVVGDEKATQHDV
                     REVIDLFRTSGSIGFAENKLTEFRDTSKRCLDSVDSSESKDLLIALADYSVTRLY"
BASE COUNT         1062 a          830 c          742 g         1220 t
ORIGIN      
        1 gttatctatt tcgggatgaa gccgattcta atgatatgtt tttaagtaag aaattgtttt
       61 aaatctgtta tattggatgt gtattatgac aaaaagtagc aaagatctga tagatcaaga
      121 tgaaaagaag ataatttctg aactcgtgaa aaactcaaaa gaaaatatag acacaattgc
      181 gaaacattgt ggtttctcag cgcagaaagt aaggagagtt attaaacaaa tggaacagaa
      241 ccatgtgatc tgggggtata cctcggtttt tgatgaaaag agaataggtc tgaagcattt
      301 tatgatgatg gcgaaaagaa cccctggaca agtcgaagaa aaaacggtag ataaaatcat
      361 ccaaagggaa ttggaacacc atgccgcaga aatagggatt actattgaaa gcagtgctta
      421 tgtccatggt gattacgact gggtcataac gttcactgct gaagacattg tccaagcaaa
      481 aatgttgtct aattcactga atctattgca tcctaataaa atcgaaaagg tgtcaattat
      541 acaatcgttg atgtttatca aaaaacaata tatcttgaat ccggatagag agatactgaa
      601 agatttctta taaactgtgt taatgctcat ccgatacgct tatataaaga tcacagtttt
      661 ttgtgttttt aggcacgttt cgtttgtttt ttaggatacg gtacttaatg tatgctctac
      721 attctctcca atggcttttc cttatttttt atctgcagaa cctccttggg ttactgaaaa
      781 agaagattct tgtttatata cagtccttgt gttatttttt tattattttt catcctcttt
      841 tgtctaatca ctatggcagc ccttatatta tatgaagatg acaatcatct tttgtaacaa
      901 ttgtattgat ttattctctt aacaaaacat ttatattctg aatggtttta tgagtattcg
      961 ctgaagagga ttatatgagg aaagttgtta ttttcacgat gctttgttta ctactgctca
     1021 taatggtccc gatgacagtt ggggtgagca ttcatcgaaa gtcagaagga atatctgcgt
     1081 ttcccgactg gactttttta caggggattg ttacacgaca tcgccatgtc catatcaatg
     1141 gtggtgagta cgtggaattt aatgcgattt tcctgcatta cagaacccac tggttcggta
     1201 acatacggtc aggttttttt catcattttg aaaaaatcgt tcttacagca tttcattatg
     1261 gttacttggg tagtcatttc gtgcttgcga aattcagcat gggattgatc ccctagtagg
     1321 acaggaataa taatcttgtg aaaaaacaat tcactctaca cggatggtgt attggaataa
     1381 atcctggtgt atcgtattgt attctcagga atatctatga ccattcttct tcgtacaata
     1441 tttcactcgc ataacacaac acccaatggc ttctgccgat attttaagaa cacgttaaag
     1501 gaccaactca aacctttgtt cgacaagggg ttttccccat aaggtacgtg ctttttcttc
     1561 ggtgagccga aaacccgtgc ttgtatacag tttatttgct gcttctaaga tgctaaccgt
     1621 ccacagaaac agtaagcgat acccattttc ttttgtgaat tgaattgccc cctggattag
     1681 tttggtacca atccctcttc tttgcagatc aggatgaacc aggaaccatc tcagctgagc
     1741 cgtggtctca tcgctcttta cgacagcaag acaaccccgt acattattac tctgttccac
     1801 gatccatatc ttttctcttg ctgtttgtgt cagagcgaac ttccgcaagg gttctgcgac
     1861 atactgctca aaggttttat caaatccata ctcctgagtg tataaagatc catgtaaaaa
     1921 aataatgtca tcgatgtcac ctgcttcgat gttgttccgt atctgaatcg ggtggatatt
     1981 ggtcataacc tttttccgta gtacacatgg cttaagattt tcccatctat tgatgcgaca
     2041 tctggtaaaa aaccccattg ctcaaaatta ttcttttcaa gtaaccttct acttccagcg
     2101 ttcttgtcta atacgatggc gaacagtatc gtatatccca atttttttgc ggtctgcatc
     2161 atgtcaatta aaagctgatt tccgaatcct tttcgtgtat actcttgtcg tatgaaaaga
     2221 cttacttcca cggttttctc aaaagccttt cttcctctcc gatagggatc gatgctagcc
     2281 caaccgacaa tcctctgatt ctgctcagca atgagtatcg gatatttctc agggggatgt
     2341 tccgtaaacc atattgttcg ttcttccacg gttgtttcgg aaagataccc tgttgatgtg
     2401 cgggtccgga ctgcttgatt gaatatttct acaatagctt gcagatcttc ttcagatgcc
     2461 gctctgatgg taatcgtcat gatcctcaga ctgaatcatc ctttttaccg tataaatcat
     2521 ttgtcatttt cggcctgtaa cctctttatg agtatcaaaa catgattaaa acgtagaaat
     2581 ccaaagaggt ctctggtgtt aatatcgaaa agactttttg atataccaca aacacaatgt
     2641 ttatatcctg tggaagaaat ctgtctgtat gaattggaga gacctgctgt tcaacgcgta
     2701 caccctggca tttatttttt tgctgatcgc attgtttatc attgcatggg cattcttaat
     2761 gtagaaaaag agaatcaata caaccttgtc acgctatagt cggccaacgc gatgagaagg
     2821 tctttacttt cagaggaatc gacgctgtca agacatcttt ttgatgtgtc tctgaattcc
     2881 gttaacttgt tttccgcaaa accaatagac ccagatgtac gaaacagatc gatgacctcc
     2941 cggacatcat gttgagtcgc cttctcatct ccgacgacct tcaacatctt ctttttgtct
     3001 ttctcatcaa cctcggataa cgcatgcaca aacatcaagg tgcgttttcc gttcaagatg
     3061 tcgctcccta tcatttgttt cccaaggacg ctttgctcgg tcaggatgtt cagcagatca
     3121 tccttgatct gaaacatcaa gccaaacagg gttccatatt cagccagatt ctccacctgt
     3181 tcgttgctgc ctttgccgat caacgcgcct cctttcccag caagataata cagctgccct
     3241 gtttttttct caatcatcct catatactgt gggatggtca catcaagccg gacagcaaat
     3301 tcaatatcta aggcttcccc ctcgtagaag tcttttgccg catccgcgat ggatcgaatg
     3361 atttccttgt aggtcactga attgagctct ggtggcgcca tgtaggagag cattcgatag
     3421 gtctccccga tcaacgcgtc ccctgcgaga atcgcgaaag gcacaccgaa tttttcatgg
     3481 gtggtctgca gacctctccg ccatttatcc ttatcgatga tgtcatcatg gataagcgtg
     3541 gaattatgca tgtactccaa cgcgatacca aacggaatcg tcgtctcagg aacaccgccg
     3601 accgcttcac aggcaagcat ggcaagaatc ggacggagcc gcttcccacc agacaagggg
     3661 atccagcgta acgcatcata aaatatctca ggctccctag gcttcagatt accggtcaac
     3721 gcacgatcaa aaatcgatgc tcgctccttg agtatctcct ctagatccat ttttcgtccc
     3781 tcagctaatg acaagttgta tctctgattc taacgttaat cctaggaact cagccatccg
     3841 tatcaccttt atgt
//
LOCUS       DATMXJ010000053         8285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_21836, whole genome shotgun sequence.
ACCESSION   DATMXJ010000053 DATMXJ010000000
VERSION     DATMXJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21836"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1366
                     /locus_tag="VN377_01575"
     CDS             41..1366
                     /locus_tag="VN377_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR27105.1"
                     /translation="MTKSSAHFIQTVFVVFIVVLLSTTAISIPYPQQNKGNTPHVTNQ
                     GSPTTSSQKISDILSMINENLILNYLQNLVGFGPRKTGTYGCEKAAKYIHEQFTENGL
                     ITRYQNWTGRGNQFIPQLFTSQNIEGTIQGTDPTDTSIIIFNAHYDSVAKGPGANDDG
                     SGTVGVLAAAYALSHFDFKRTVKFVTFSGEEVGLLGSQAYTKEAYERNDNILVEINAD
                     MIGHDEGSGTMTATTTEDAGWVADIFQSMNANYSIGLTVNRGTINRAGHGLGGSDYSA
                     FIPYSWESVCCWEGDHDPNFHSPQDNLSNINISYLVNMTRIIAATLAYLADMDQTPPQ
                     IRITSPRVGFLYIDGMQKRPLDEFKTTVINNIWIWAEVDNATIPIQRAEFYYDGKLVY
                     TDTEAPFMWEFNKFSLRKHQITVVMYDQLGRNSSDWREIRFINIFKRIS"
     gene            1443..3404
                     /gene="rqcH"
                     /locus_tag="VN377_01580"
     CDS             1443..3404
                     /gene="rqcH"
                     /locus_tag="VN377_01580"
                     /inference="COORDINATES: protein motif:HMM:NF041120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HWR27106.1"
                     /translation="MERGLASFDIYLIVSELQDLRGCFVEKIYQLSRDEILLRVQKKK
                     ESQKEALFIRNGEFFCRTQRSFDTPEKPSLFAMTLRKYLLNGKISEINQHEFDRIIQI
                     KIGRKEGDYTLVCELFSKGNIILLNPEGRIIRPLITKEWASRVIRSGEIYQPPPSQTN
                     PFHISEPAFQELLTKSSKDLVRTLASSINLSGMYAEELCLRAGVDKSTKTKTIDAITM
                     KKLYDELQKLLSIFQEKNIHPVFVKKDGDLIDILPFPFLSYAAIDYEPTASYSKGLEL
                     FIPQQKTQKPQETAHRKMTEKRQRQLMQQQELIEEFKKNIEQKKREANILYLNFQPCE
                     TLLQEISVLLRQKEKNDAINKIRANPVVKLFDPTTNELIILLNDEEGNTIEIGLDFRK
                     NVSENANQKYEESKKIQEKLKGALEAVEQTKHDLATIKDVEVIEKKNKVTHEKQWWFE
                     RFRWFISTEGNLVVAGRDAASNDTVVKKYLSAGDRYAHADIHGAPSCVIKSKGLNDEK
                     IPISEKTLEEACMFAASYSRAWNQFGEASAYWVLPEQVSKTPESGEFVPKGGFIIRGK
                     RNYYRSKLEVAVGLIPFGDGEKLMGGPVNAVAARALKGYAILTPGTMKKSMIARRLAK
                     AFQVSTDLVEKVIPPGECTIVKTIGFEVL"
     gene            3415..4461
                     /locus_tag="VN377_01585"
     CDS             3415..4461
                     /locus_tag="VN377_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HWR27107.1"
                     /translation="MKVIFKDPRSGEIKLIPENLDDIWHLYNIIEEGDLVRAVTFRTA
                     EGEKADKLRAKKTEKKKMKLGIRVEKVNFHEFSNRLRIQGVIEEGPQDLGSYHTFNVD
                     AEEMQPLSIMKERWKAHQLQRIDEAVLQRTQPMLVFVSLDDDSATVALLYQSGVQLVA
                     EIDAHRSGKMYESKETTPEYYDEIFSVIKTVKKSDSPLVVIGPGFTREHLMKAGKEKE
                     QELFKDCITHATANAGMNGVHEALKVGIVEQITKENRVSKETQAVENVFEQIKKDGPV
                     TYGMKEVEDALSRGAAEHLLISDVLVRTNNGEHLLDLARKTHCDFMIINTMHETGKKF
                     DGIGGVAALLRFKY"
     gene            complement(4470..5240)
                     /locus_tag="VN377_01590"
     CDS             complement(4470..5240)
                     /locus_tag="VN377_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27108.1"
                     /translation="MVSLKKNDKIIIIVAVVILVIAGVGVAMYQSPKAPTDYSSLITS
                     EKSFNVTWALRNGTLTSISDFTGKKTPFEGIVTIPEGNVNSVTFNLSWTDDHMTFLKR
                     MGLDKLTLEVSMPDGMNSFSETNTSAPKTGYGTISYTISKDINPPEGPIKAENEQEAQ
                     TKLQTAPFFDDSWTDKDIKINVSVQIGELRFLKKLRDKGNDFELQISYQYYDGTLNED
                     TTKNTGGGSDMPPEDIWSSNEEPPYISMIINTGCGRFV"
     gene            complement(5376..6476)
                     /gene="amrS"
                     /locus_tag="VN377_01595"
     CDS             complement(5376..6476)
                     /gene="amrS"
                     /locus_tag="VN377_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HWR27109.1"
                     /translation="MNTRIEAYSEAEANSSYKNINKASFIENSAMKKEALFWKPLKNQ
                     IVQCFLCPHHCIIAPEKHGICGVRKNEHGTLFSLIYQSCSSIAVDPIEKKPLYHFYPG
                     SLVLSLGSVGCTFRCDHCQNYNISMARPEEQSLRDIPAEQLSTIAKEKNCAGVAWTYN
                     EPTIWHEYTIEAAKKVKDAGLYTVYVTNGYIEEEPLKEIAPYLDAMNIDVKAFNEEFY
                     KKICKAKLAPVLQTCERAKTIGVHLELTYLVIPGHNDTPSEIKEFCQWIAEKLGVETP
                     VHFSRFHPDYKMTDTLATPLSTLLACHTAAKDAGLQYIYLGNIGHGDYDNTYCPSCRN
                     LLIERYGFSALIKGLAQGTCSRCGTVIPLQMG"
     gene            complement(6424..7728)
                     /locus_tag="VN377_01600"
     CDS             complement(6424..7728)
                     /locus_tag="VN377_01600"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27110.1"
                     /translation="MRYKWLIALTVIVVFLLLISNYLGIFSTNLNFNTNKPTVSITYP
                     SDGATVSKLVMISGSASNPNGDKDINSVQIKIDGNNWVTVTGTTLWSYDWTTYTLPDG
                     QYNISARSYNGKDYSETVSITVRVNNPASVDSGTHKWAIFIVAANFPKNNDSKLGNGG
                     LYLAEQMAAFFIENYQYPTSQIMILFDDGWIRSDNGYGTKQKTLQERTHEYDISYGGA
                     TKTNVIASLQYLINESNAYPDSEVFIWIFNHGVGNSNKTFTGGKILQRSEIFLWDDTI
                     TDKELGDILAPLESKKVAIIIDACYTGGFADRTILSFRTSLLLRSGIPQNGRVVISGT
                     SKFRTGYASTIQGPIFTLLWFEGLSTGYADGYRPGLFDRGVLRKMKVFQDGQASVEEA
                     FYYARALLRTDEKYQDFKSMQPQINDEYPHRGLFRSRGELIL"
     gene            complement(7801..>8285)
                     /locus_tag="VN377_01605"
     CDS             complement(7801..>8285)
                     /locus_tag="VN377_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWR27111.1"
                     /translation="LGYYTLQRNKKEKKLSKALLEPQPRLKEGRLLAQLHSVTSSMDL
                     SDGLSASLYQLQEINPVGFEIDKNVLPLASGLNQRFHADYGADPYSLALHFGGDYELL
                     MTVTPQKFERTKRTLQKHGVTFTSIGTVTQKRKIILLENGKKKILPNKGYEHFKKHDL
                     "
BASE COUNT         2412 a         1858 c         1853 g         2162 t
ORIGIN      
        1 ctatataata ttgatgcttc ttataagata aatagaaaac atgacgaaat catcggctca
       61 tttcatacaa actgttttcg ttgtattcat agttgttctc ctaagcacca cagcaatatc
      121 catcccctat ccacaacaaa acaagggaaa cacaccacac gttacgaatc agggttcacc
      181 gacaaccagc agtcaaaaaa tctcagatat tctgtccatg ataaatgaaa acctcattct
      241 gaactatctt caaaacctcg ttggttttgg acctcgtaag accggaacct acggatgtga
      301 aaaagcagcg aaatacatcc atgagcaatt cacagaaaat ggtctcatca cacggtacca
      361 aaactggaca ggaaggggaa atcagttcat cccccaactt tttacaagcc aaaatataga
      421 aggaacgata cagggaacgg acccgactga tacatcaatt attattttta atgcacatta
      481 cgattcagtc gcaaagggac caggagctaa cgacgatggt tcaggtactg ttggtgtact
      541 agcagcggcc tatgctctca gtcattttga ttttaaacgt accgttaaat ttgtcacttt
      601 ttcaggggaa gaagtcggat tactcggaag ccaggcttac acaaaggaag cgtacgaacg
      661 aaacgataac attcttgtgg aaatcaatgc agacatgatc ggacatgatg aagggagcgg
      721 aacgatgact gccaccacaa ccgaggatgc tggatgggtc gcagatatct ttcagagcat
      781 gaatgcgaat tattcgattg gactgaccgt caatcgcgga acaatcaacc gagcgggcca
      841 cggtcttggc ggtagtgact actcagcatt tatcccatac agctgggaat ctgtctgctg
      901 ctgggaagga gaccatgacc caaacttcca ttcaccacaa gacaacctga gcaacataaa
      961 cataagctac cttgtcaata tgaccaggat catcgcagcg acccttgctt atctggcaga
     1021 catggaccaa actcctccgc agatacggat cacctcgcca cgcgtcggat ttctgtatat
     1081 cgatgggatg caaaaacgcc cactcgacga gtttaaaaca acggtcatca acaacatatg
     1141 gatctgggcg gaagtcgaca atgcaacaat ccccatacaa cgagctgagt tctactatga
     1201 cggaaaatta gtctatactg acaccgaggc gccatttatg tgggagttca acaaattctc
     1261 cctgcgaaaa caccagataa cggttgttat gtacgatcaa ttagggagaa actcctcaga
     1321 ttggagagaa atccgtttca tcaatatttt taaaagaatc agttaatcaa accaccccat
     1381 cttttcccca tcaatccatc ctattctttt ttaacattga aatagataat ggcccgtaca
     1441 atatggaacg aggactagca tcattcgaca tctacctcat cgtctcagaa ctccaagacc
     1501 tgagaggatg tttcgtagaa aaaatctatc aactctctcg cgacgagatc ctactgagag
     1561 tccaaaagaa aaaagaaagt caaaaagaag cactgtttat ccgaaacgga gaattctttt
     1621 gccgtaccca gaggtcgttt gacactccag agaagccctc cttgtttgcc atgactctac
     1681 gaaaatacct gctgaacgga aaaatcagcg aaatcaacca acatgaattc gacagaatca
     1741 tacagataaa gatcgggaga aaagaaggag actatacctt ggtatgcgag cttttctcca
     1801 agggcaatat cattcttctg aacccagagg gaaggatcat ccgcccgctc atcacaaagg
     1861 aatgggcgtc ccgagtgatc agaagcggtg agatctacca accaccacca tctcagacga
     1921 acccgtttca tatctccgaa cctgcattcc aagaactcct cacaaagagc tcaaaagacc
     1981 ttgtacgaac ccttgcatct tccataaacc tcagtgggat gtacgccgaa gaactctgct
     2041 tgcgtgctgg cgttgacaaa tctacaaaaa cgaaaacgat cgatgcaatc accatgaaaa
     2101 aattatatga cgaactacaa aaactcctat cgatatttca agaaaaaaat attcatcccg
     2161 tgttcgtcaa aaaagacggg gacctcatcg atatcctccc gttccccttt ctcagttatg
     2221 ccgctatcga ctatgaacca acagcgagct actccaaagg ccttgaacta ttcatcccac
     2281 agcaaaaaac acaaaaacct caggaaacag cgcatcgaaa gatgaccgaa aaacgccagc
     2341 gacagctgat gcaacagcaa gaattaatcg aagagttcaa aaaaaatatt gaacagaaga
     2401 aacgagaagc caacatcctg tacctcaatt ttcaaccctg tgaaacactc ctccaggaaa
     2461 tctcagtcct gctcagacaa aaagaaaaaa acgatgccat caacaagatt cgcgcaaatc
     2521 ccgtagtaaa actgttcgat cccactacca acgaactcat catcctgctc aatgacgaag
     2581 aaggaaatac gattgaaatc ggtctagatt tccgaaaaaa tgtttctgaa aacgctaacc
     2641 agaaatacga ggagagcaaa aaaatccagg agaaattgaa aggagcactt gaagcggtcg
     2701 aacagacaaa acatgaccta gccacgataa aggacgttga agtcatcgaa aagaaaaaca
     2761 aagtaaccca tgaaaaacaa tggtggtttg agcgattccg ctggttcatc tcaacagaag
     2821 gaaacctcgt ggttgctgga cgagacgcag caagcaatga taccgtcgtg aagaaatacc
     2881 tttcagcagg ggaccggtac gcccatgcag acatccacgg cgcacccagc tgtgtcataa
     2941 aaagcaaagg cttgaacgat gaaaaaatcc ctatctctga aaaaaccctc gaagaagcat
     3001 gtatgttcgc agcttcctat tcgcgcgcat ggaaccagtt cggtgaagcc tccgcgtact
     3061 gggtgctgcc ggaacaggtc agtaagacac cggagagcgg ggagttcgtc ccgaaaggcg
     3121 gatttatcat cagaggaaaa cgcaattatt atcgaagtaa actcgaggtc gctgttggtc
     3181 tcatcccctt tggagatgga gagaaactca tgggtggacc tgtgaacgct gttgcagcgc
     3241 gggcgctgaa aggatacgca attctaaccc ctggcactat gaagaaaagc atgatagcac
     3301 gaagacttgc aaaagcattt caagtctcga ccgatttggt cgaaaaagta atcccaccag
     3361 gtgagtgtac tattgtaaaa accataggat ttgaagtact ctaggtgaac agctgtgaag
     3421 gttatcttta aagaccccag aagcggtgaa ataaaactga ttcctgagaa ccttgatgac
     3481 atctggcatc tgtataacat catcgaggaa ggggatttgg tccgagcagt gaccttccgt
     3541 accgcagagg gtgaaaaagc agacaagctc cgtgcaaaaa aaacggagaa gaaaaaaatg
     3601 aaactaggga tccgcgtaga aaaagtaaac ttccatgagt tctcaaacag acttcgcatc
     3661 caaggagtca tcgaggaagg tcctcaggat ctcggttcgt accatacatt caatgtcgat
     3721 gctgaggaga tgcaaccgct ctccattatg aaagaacgat ggaaagcaca tcagctccag
     3781 cggatcgatg aggcagtcct acagcggacg cagccgatgc tggtgtttgt gtcccttgat
     3841 gatgattctg caacagtggc tctcctgtat cagagtggtg tgcaacttgt cgctgagatc
     3901 gacgcgcatc ggtccggcaa gatgtatgaa agcaaagaga ccacgccgga gtattatgac
     3961 gagattttct ctgtgataaa gacggtaaaa aaatctgatt cgcctcttgt cgtcattggt
     4021 ccagggttta ctcgggaaca tctcatgaaa gcaggaaagg aaaaggaaca agagctgttt
     4081 aaggattgta tcactcatgc gaccgcgaac gcagggatga atggcgtcca tgaggcactc
     4141 aaagtgggta tcgtcgaaca aatcacaaag gagaatcgtg tgtcgaaaga aacccaggct
     4201 gttgagaacg tgtttgagca aatcaaaaaa gacggcccag tgacgtatgg aatgaaagaa
     4261 gtcgaagacg cactctcgcg aggtgccgct gaacacctgc ttatctcaga tgtactggtt
     4321 cgaacgaata acggggaaca tctgcttgat cttgcgagaa aaactcactg tgatttcatg
     4381 atcatcaaca cgatgcatga gacaggaaaa aaattcgatg gaatcggtgg tgtggcagcg
     4441 ctgcttcggt ttaaatatta aaatttctgt tagacgaacc gaccgcaccc agtgttgatt
     4501 atcatggaga tgtagggtgg ttcttcatta gaggaccaga tatcttcagg tggcatatct
     4561 gagccgccgc cagtattttt cgtggtatcc tcgttcagtg taccatcgta gtactgatat
     4621 gaaatttgta gctcaaagtc attacccttg tcacgtaatt ttttcaagaa gcgcagctcc
     4681 ccaatctgga cgctgacatt gattttaata tccttatcag tccaggagtc atcaaaaaag
     4741 ggggcggtct ggagttttgt ttgtgcttct tgctcatttt ccgcttttat tggtccctca
     4801 ggtgggttga tatctttaga gatagtatag ctgatagtcc cgtatcctgt ttttggtgca
     4861 ctggtatttg tttctgaaaa tgaattcatc ccatctggca tgctgacttc caaagtcaat
     4921 ttgtccaatc ccattcgttt cagaaatgtc atatgatcat cagtccagct gaggttaaaa
     4981 gtgacactgt ttacgttacc ttcagggatg gtgactatcc cctcgaacgg ggtttttttc
     5041 ccagtaaaat cagaaatcga tgtaagagtg ccgtttcgta atgcccaggt aacattaaag
     5101 ctcttctcac tggttatcaa tgaggaataa tctgttggtg cttttggtga ttggtacatc
     5161 gctacgccta caccagcgat aacaagaatc accacggcaa caatgataat gattttatca
     5221 ttctttttta aggataccat aaagtcacct gtaccattat tactttattg ctatgataat
     5281 atataaagat aagtatgctt cctcctttat taaatttttc ctcaatatat atatttaata
     5341 aggtttaaat caaacaggag gaaaaaacag atgaatcacc ccatctggag agggatgacg
     5401 gtcccacacc gtgaacacgt tccttgggct aatcctttta tgagtgcaga aaagccatac
     5461 cgttcgataa ggaggttcct gcaggaggga cagtaggtgt tgtcgtagtc cccatgaccg
     5521 atatttccaa gatagatgta ctgtaaacca gcgtcttttg cagcagtgtg acacgcaaga
     5581 agagtgctca gaggtgttgc tagggtatca gtcattttgt aatccgggtg gaacctggag
     5641 aagtgcaccg gggtctctac cccaagtttc tctgcaatcc attggcaaaa ctctttgatc
     5701 tcgcttggag tatcattatg accgggtatc acaaggtagg ttagctcgag atggaccccg
     5761 attgtctttg cacgctcaca ggtttgtagg actggtgcca atttcgcttt acagatcttt
     5821 ttgtagaact cttcgttgaa cgctttgaca tcgatgttca tcgcatccag atacggggca
     5881 atttctttca ggggttcctc ttcgatatat ccattggtaa catagacggt gtacagaccc
     5941 gcgtccttga cctttttggc agcttctatc gtgtactcat gccaaatcgt tggttcgtta
     6001 taggtccagg cgacacccgc acagtttttt tccttagcaa tcgtactgag ttgctctgca
     6061 gggatatcgc gaagagactg ttcctctggc cgtgccattg agatattata gttctgacaa
     6121 tggtcacatc tgaaggtgca tccaacggaa cctaacgaga ggactagaga ccctgggtag
     6181 aaatgataca atggtttttt ctcaattgga tcaaccgcaa tcgaagaaca cgactgatag
     6241 ataagactga acagtgtccc gtgctcattt tttctcacac cgcaaatccc atgtttttca
     6301 ggggcgatga tgcaatgatg tggacagaga aaacattgca cgatctgatt tttcaagggt
     6361 ttccaaaaca atgcctcttt tttcattgca gaattttcaa tgaatgaggc tttattaatg
     6421 ttcttatagg atgagttcgc ctcggcttcg gaataagcct ctatgcgggt attcatcgtt
     6481 gatctgcggc tgcatggatt tgaaatcctg atatttctca tcggtgcgaa gcagagccct
     6541 cgcatagtag aatgcttctt cgacggaggc ttgtccatcc tggaatacct tcatttttcg
     6601 gaggacccct cgatcgaaca atccaggtct gtaaccgtcc gcgtaccctg tggagagtcc
     6661 ctcaaaccat aacaacgtga aaatcggacc ttggatcgta cttgcgtacc cggtacggaa
     6721 cttgctagtc cctgagatca ccacgcgtcc gttttgaggg atacctgatc gcaataacaa
     6781 tgaggtccgg aagctgagaa tcgtccggtc tgcgaaccct ccagtgtaac acgcatcgat
     6841 gattatcgct actttcttgg attccagggg cgcaaggatg tctccaagtt ctttgtcagt
     6901 gatggtatcg tcccagagga aaatctcgct tctctgcagg atttttccgc cagtgaatgt
     6961 tttgttgcta ttgccgactc catgattgaa aatccagatg aatacctcac tatctgggta
     7021 tgcattggac tcgttgatga ggtactgcaa ggaggcgatg acgtttgttt tggtcgcacc
     7081 tccataggag atatcgtact catgagtgcg ttcttgcaat gttttttgtt tcgtgccata
     7141 tccgttgtca ctgcggatcc agccatcatc aaaaaggatc atgatctggg atgtcggata
     7201 ttggtagttt tcaataaaga acgctgccat ctgctcggcg agatagagac cgccgttgcc
     7261 tagtttcgag tcattgtttt ttgggaagtt tgctgctacg atgaagatcg cccatttatg
     7321 ggtaccagag tcgacagagg ctgggttgtt caccctcact gtgatcgata cggtctcaga
     7381 gtaatccttc ccattgtagg atcgtgcact gatattgtat tgtccatctg gtaaggtgta
     7441 ggtcgtccag tcatagctcc agagtgttgt tcctgtgacg gtcacccagt tattcccatc
     7501 tattttgatt tgtacgctat tgatgtcttt gtctccatta gggttgcttg ctgacccgct
     7561 aatcatcacg agtttggaga cggttgcccc gtcggatgga taggtgatgg aaacagttgg
     7621 tttgttggta ttgaaattta aatttgtact aaaaatccct aggtagttac taatgagtaa
     7681 taggaagacg acgatgactg taagagcgat tagccatttg tatctcattg ttctcagtat
     7741 actatgtgtc tctctactcc tttaaaacct ttgttgaaca gccagtacct gtttgtcttg
     7801 ctacaaatca tgttttttga aatgttcgta tcctttgttg ggcaatatct tctttttccc
     7861 gttttcaagc aggataatct tccttttttg tgtaacggtc ccaattgatg tgaaagtaac
     7921 accatgtttt tgtagcgtcc gtttggttct ttcaaatttc tgcggtgtga cggtcatcag
     7981 cagttcgtag tccccaccaa aatgcaatgc aagagagtac ggatcagccc cataatcagc
     8041 atgaaaacgc tgattcagtc cggaggcgag cgggagaacg tttttatcta tttcaaatcc
     8101 gacagggttt atctcctgca gctgatacaa ggacgctgac aagccatcgg agagatccat
     8161 ggatgatgtg actgaatgta gctgtgccaa gagccgaccc tctttcaatc ttggttgagg
     8221 ctctaacaga gccttggaga gttttttttc ttttttgttt ctttgaagcg tataatatcc
     8281 aaggc
//
LOCUS       DATMXJ010000054         8618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_27002, whole genome shotgun sequence.
ACCESSION   DATMXJ010000054 DATMXJ010000000
VERSION     DATMXJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27002"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="VN377_01610"
     CDS             <1..1830
                     /locus_tag="VN377_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27112.1"
                     /translation="MIVGKPRAQSLMTVRDESSLGFAIDLVNFNQEYRGAFWYVFGDT
                     VIVNTLSDARRLMGGVRLVDMKGDLIEASGAMVGGSPPSERLSFGSADQQTLDEITRK
                     LNTTIQHQDELSEELAQLKKDIGELENVFRTLKGETDAGTQTKDLEIRKKEFHGKLDI
                     LSKDLDGKLKEKQVIEAESGAILVSLNECEKRLKELDVLKEEKGKHLLKSTKKEVAQA
                     VRSLEKTVSEFQENRLKLHSEQETIQKKIELLNERKIELTQKISTIDTDVETLKKTVQ
                     ELKETRGKGQNELKALMTVEETMSGKTKELAVQRDTLYKKTVGIENELESIGTKTESY
                     LDLIARAKYRLPTLDDSLKELDQEIELYHVEMKYTKLPTIESMKETILSVEQTMQGLE
                     PVNMRALEEYEHQMERKKKFDEDVDHLNIQKKNLVKLVGEITSKKKERFFEVYDEINK
                     NFKEIYGQLSEGGEAELLLENEENVFEGGLTIKARPHGKKVLRLAALSGGEKSIASLG
                     FIFSIQQYDPSPFYVLDEIDMFLDGVNAEIVSRMIKNRAEHSQFISVSLRKIALKEAN
                     YLYGVTMHDSGISEMIGNIDPDSVGPKGEIKTIVQEVLPHGAG"
     gene            1817..2683
                     /locus_tag="VN377_01615"
     CDS             1817..2683
                     /locus_tag="VN377_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWR27113.1"
                     /translation="MELDNDIINHLLFHKALIDENNDMTRINQYLEMAKAAATGESTS
                     IQDPFDRSVYLAFDLVLNQNMNPWDIDLVGFSTMYLKRAKKEKIDLLTAGKIIYMAWK
                     VLRMQSDHLVVNMETKEEEIDQGFGWEDIPNSSWLANDDEYSYTNLLMKMPQPPLEEP
                     LRRDSTRKVTLIELLSAFDEVRKESEQYQLLDHLRQEERILLEKKAWKAMKGSAHEDH
                     LEEDVATVWERICQFPKKTMNFNELCEVESAEERIKTFISILFLAYEKKIIVHQQKFP
                     YGEIFIKTIGFT"
     gene            2697..3314
                     /locus_tag="VN377_01620"
     CDS             2697..3314
                     /locus_tag="VN377_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWR27114.1"
                     /translation="MSIKEDRVVESLLFSAGKPLSVEEIQETTGLLPKHVTDAIDHLM
                     QSYNIDRKNDTSLEIVKAGNKFIMQVKKTFTDKSMMVAKPEIRTDLLKTLALIAFHQP
                     VKQSNLRHMIGERIYGDVDQLTSLNLVHSEPHGATEVLTTTRLFPEYFGIDSTKPEEI
                     REFLAKKVIGHVVKEKQNEPLDKEESSADEEATEEEIPQTQMKTE"
     gene            3424..4122
                     /locus_tag="VN377_01625"
     CDS             3424..4122
                     /locus_tag="VN377_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWR27115.1"
                     /translation="MVNCPKCGKINSDDAAFCTSCGESIHTDVGSTIERHAKQFAQNM
                     EQAGKKVGDQMAQAAKKVHETTQKEAQHFEQRMDRVSHRAESWYERRFGALGPLLESF
                     IFLIVFRLIIMVMELPNEETPEVRTVAAILIVYILPLFVLSLLSNYTQYLSRKFFQIK
                     VFSPLLYATFFVLLCWIISKILNDASSHFTIPHLQTAALSLENSLPTIFVFVLLLGYV
                     ILALNLPKDHWKKP"
     gene            4177..4524
                     /locus_tag="VN377_01630"
     CDS             4177..4524
                     /locus_tag="VN377_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWR27116.1"
                     /translation="MVYCPKCGIKNEDDATFCKSCGASLTGSRQESDRHRDQRCEEDC
                     AGGHGGRGWAMFWGVIIVLIGLVILFEVVIKDMAKTYSWLSWVNTVQWNWIFAFVIAI
                     FIIIFGLRVLSRR"
     gene            complement(4521..5234)
                     /locus_tag="VN377_01635"
     CDS             complement(4521..5234)
                     /locus_tag="VN377_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27117.1"
                     /translation="MKHIRIIPLYIAFSSIAAVLLIFIVGTISSQASPLSVSEKIGIA
                     SVFILCCVIGISFTVRPNWTRRYFHKVKNEEKDTQSLRQRFFRGHHPDCLAFQNHTIY
                     WRKKTWCAGCLGLFIGLCASIILMILYLRTDFQPTRMISLALLLIGLFILAVVYIEIL
                     HRSKRATVHVFINSLLPLSFLSITIAVGGATREFIYCLFSILLCFLWLDTRVQLSKSH
                     HRSLCRNCVESCKMFTASV"
     gene            complement(5231..6658)
                     /locus_tag="VN377_01640"
     CDS             complement(5231..6658)
                     /locus_tag="VN377_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27118.1"
                     /translation="MKSSLLFVEPPRNFWFVMGEYLPPPSSLLILAAYVERELPGIDI
                     DIVDCQADRLDWKGLEKYIESTDPSIVLTSGFTTNAYTCARTCEIAKTVNEDIATIVG
                     GIHFSFATDESLRNYPEIDYIVRGEGEQTVVDLIKTLQNKGKINDVQGISYRKNNTIV
                     HNPTRPLIENLDTLPYPAYHLVEKNLKRYHFTMMAGRNTRYMILEGARGCEHQCSFCT
                     QWKHWGSRWRTKSAKRIADEIEYLNDTYGGVFLWLADDNLRLSHRGKQLYEELRQKSC
                     KDDIMLFFQARIDDIAQHPDLIGNLREVGTYWILCGVETNSPEMLHEYKKGIKTQDAY
                     QAMKILKQKDIFSQAMFVIGGRKDTHESIEQLRQYSVDLSPDIAIYTALTPFPGTIYY
                     ETAKKNGWIQDTNYANYDMAHAIMPTETLTRKEVQEELWRCYRMFFGSLTRSIIGVLA
                     KNKLKRTMYQHMAGQFVLEKFRRLI"
     gene            complement(6757..8511)
                     /locus_tag="VN377_01645"
     CDS             complement(6757..8511)
                     /locus_tag="VN377_01645"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27119.1"
                     /translation="MKKNNLGKAAVSFFVLFVLLITSVQATDTGLVSKNNKKMESRGW
                     YWKPSYPNYVPQGLPDFSQQQDQWKKIAPGPNNMIDSTVAGDDIYNPSENCIAPGPDC
                     ALNSTAVGDDVVEWAFCGPVAVANCFWWFDSKFADPEGTPGDGQDQFALVQDYGIGDD
                     HTTTNVPLLIQNLARAMNTTEKGTTYVDDMHSAISDWFTTTGLTDKFTVQTYDRPTFS
                     FIEGEIERSQDVILLLGDYNYIIGPLMVDQMQLLGPVLKVLQINTLWDSQGFIPTVTR
                     LDAIEILLQSVSTFPPTDVEVNVYDNRPGNLLGKSTVNPGFLPGQTWIRFDFNPSISL
                     TPGATYYFDVRQLVEDPHYYWSYDVGNPYPPGQAWKDNVLSSIDWAFKTEYYNPPPHS
                     ERKGGHYVTCAGVNSEDSMIAFSDPIVDIANPAPNDHNDAAYVSHDIYNVSVDAPQPD
                     INTQWWLTDYDAGYNYTVVEQAVVICPVPDTTPPSLQITKPEYALYFLNKEIMPLSGG
                     TLIIGKIDVNVNATDDDSGIDRVEFYVNLQLLGNDTTAPYSWSWSQPAFFRQTLTVKA
                     FDKEGNVDSKEIEVWKFF"
BASE COUNT         2556 a         1782 c         1966 g         2314 t
ORIGIN      
        1 atgattgttg ggaaacctcg cgcacaatcc ctcatgacag tacgagatga aagctccctt
       61 ggatttgcca ttgacctggt gaacttcaat caggagtacc gaggagcatt ttggtacgtg
      121 tttggggata ccgtgattgt gaataccttg tctgatgcac gacggttgat gggtggcgtc
      181 agactcgtgg atatgaaagg ggatttgatt gaagccagtg gtgcaatggt cggaggcagt
      241 ccaccaagtg aacgtctttc ctttggcagc gcggatcaac aaacccttga tgagattacc
      301 aggaagttaa atacaacgat tcaacatcaa gacgagttat ccgaggagct tgcgcagttg
      361 aaaaaagaca tcggagagct ggagaatgtc ttccggaccc tcaaaggcga aaccgatgca
      421 gggacgcaga caaaggatct tgaaatccga aagaaagagt tccacgggaa acttgacatt
      481 ctttcaaagg accttgacgg aaaactcaaa gaaaagcagg tgattgaagc agaatctgga
      541 gcgatccttg tatcacttaa tgaatgtgaa aaacgtttaa aagaattaga tgtcttaaag
      601 gaagaaaaag gaaaacacct cctgaaaagc acgaaaaaag aggtcgcaca agcagtccgc
      661 agtcttgaga agacagtctc agagttccaa gaaaataggt taaaactcca cagtgaacag
      721 gagactatcc agaagaagat tgagctgtta aatgaacgaa aaatcgagct tacccagaaa
      781 atcagtacga ttgatacaga tgtcgagacc ttgaaaaaaa cagttcaaga gctcaaagaa
      841 acccgaggga aaggtcaaaa cgagctcaaa gcactcatga ccgtcgagga gacaatgagt
      901 ggaaaaacca aggagctggc agtgcagcgc gatacgctgt ataagaaaac cgttgggatt
      961 gaaaacgagc ttgaatcaat tggcacaaag acagaatcct atcttgattt gattgcgcgg
     1021 gcgaaatacc gcttgccaac tcttgatgac tccctcaagg aacttgacca ggagatcgaa
     1081 ttatatcatg tcgaaatgaa atacacaaaa ctcccaacaa tagaatccat gaaagaaaca
     1141 atcctctctg tcgaacagac catgcaagga ctggagccag tcaacatgcg tgcccttgag
     1201 gagtacgagc atcagatgga gcggaaaaag aaatttgacg aggatgtcga ccatctcaat
     1261 attcagaaga agaacctcgt aaaactcgtt ggtgagatca ccagcaagaa aaaagaacgg
     1321 tttttcgagg tgtacgacga gatcaacaaa aacttcaaag aaatctatgg acagttatcg
     1381 gagggtggcg aagcagaact attactggag aacgaagaga acgtcttcga aggaggtctt
     1441 accatcaaag cacgacctca tgggaagaag gttttacggc tcgccgcgct ctctggtggg
     1501 gagaaaagca tcgcttcgct cgggttcatc ttttcgattc aacaatacga cccaagcccg
     1561 ttttacgtac ttgatgagat agacatgttc cttgatggtg tgaatgcgga gattgtctca
     1621 cgcatgatta agaatcgagc ggaacactcc cagttcatca gtgtatcact ccggaagata
     1681 gccctcaagg aggcgaacta tttgtacggg gtcaccatgc atgattccgg tatctctgag
     1741 atgatcggta atatcgaccc ggattccgtg ggacctaaag gagaaatcaa gacgattgtg
     1801 caggaggtgc tccctcatgg agctggataa tgacatcata aaccatttat tattccataa
     1861 ggccttaatc gatgagaaca atgatatgac ccggatcaac cagtaccttg agatggccaa
     1921 agcagcagcc acgggagagt caacatcaat tcaggacccg tttgatcgat cggtgtacct
     1981 tgcatttgat ctggtgttaa accagaatat gaacccatgg gatatcgatc ttgttgggtt
     2041 ctccaccatg tacctgaaac gggcgaaaaa agagaaaatc gatttgctga ccgctggaaa
     2101 gatcatctac atggcctgga aggtactgcg gatgcaaagc gatcatctcg ttgtgaatat
     2161 ggagactaaa gaagaagaaa tcgaccaggg attcggctgg gaggatatcc cgaattcctc
     2221 atggctcgcc aatgatgacg agtactctta caccaatctt ctcatgaaaa tgccacagcc
     2281 ccctcttgag gaaccgttac gacgtgattc aacccggaaa gtgaccctta ttgagttgct
     2341 cagcgccttt gacgaggtgc ggaaagaatc agaacaatac caattgcttg atcacttacg
     2401 acaagaagaa cgaatcctcc ttgagaaaaa agcatggaaa gcaatgaaag gctcagccca
     2461 tgaggatcat ttagaggagg atgtcgcaac tgtctgggaa cggatctgtc aattccctaa
     2521 aaaaaccatg aatttcaatg agctgtgtga ggttgagagt gctgaggaac gaatcaagac
     2581 gtttatctct atcttattcc tcgcatatga aaaaaagatt attgttcatc aacagaaatt
     2641 cccatatgga gaaattttta ttaaaacaat agggtttaca taggtggaga acaggtatga
     2701 gcattaagga agatcgcgta gttgaatcat tgttattctc tgcaggaaaa ccactcagtg
     2761 ttgaggagat ccaggagaca acagggttat taccaaaaca tgtcacagat gctatcgatc
     2821 atctcatgca gtcctataac atcgacagga aaaatgacac ctccttggag attgtgaaag
     2881 cgggcaacaa gttcattatg caggtgaaaa aaacgtttac tgataagtca atgatggtgg
     2941 caaaaccaga aatccggacc gatttgctca aaaccttggc gttgattgcg tttcatcagc
     3001 cggtgaaaca atcgaacctg cgccatatga tcggggagcg gatctatggt gacgtcgatc
     3061 agttgacgag tttgaatttg gttcattcag agccgcacgg cgccacagaa gtcctgacaa
     3121 cgacacggtt gttcccagag tattttggta tcgattcgac aaaacctgag gagattcgag
     3181 agtttttagc aaaaaaggtc atcgggcatg tggtgaagga aaaacaaaac gaacctttag
     3241 ataaagaaga atcatcagca gatgaagagg ctaccgaaga ggaaattcct cagacacaaa
     3301 tgaaaacaga ataaaataat cgtggttttc caagtgttgt agcgtcagaa tctaccattt
     3361 ttttcgaaga ggtatgtttt taaagattga tttgatatga tatcatagcc tggagagaaa
     3421 tgtatggtga attgtccgaa atgcgggaaa attaacagtg atgatgcagc attttgtacg
     3481 agctgtggtg agtctataca taccgatgta gggtccacca ttgaacgaca cgcgaaacaa
     3541 ttcgcacaaa acatggaaca ggctggcaaa aaagtcggag accagatggc ccaagctgca
     3601 aaaaaggttc acgagacgac ccaaaaggaa gcacaacact ttgaacagag aatggatcgg
     3661 gtcagtcacc gtgcagagag ttggtacgaa cgccgctttg gtgcgttagg accattgcta
     3721 gaaagcttca tttttttaat tgtgtttcgt ctcattatca tggtcatgga gctaccgaat
     3781 gaagaaaccc cggaagtacg caccgtggcc gctatcctca ttgtatatat cttaccgctc
     3841 tttgtgttga gcctgctttc aaattacaca caatatcttt caagaaaatt ctttcaaatc
     3901 aaagtgttct cgcctctttt gtacgctaca tttttcgttc ttctttgctg gattatttca
     3961 aaaattctta acgatgcgag cagccatttt actatcccac atcttcagac tgcggcgctg
     4021 tcacttgaaa atagtctccc gacaatattt gtctttgtcc ttctccttgg gtatgtgatt
     4081 ttagcgttaa atttgccaaa ggatcattgg aaaaaaccat aaagaaggat ataaatagaa
     4141 tgaatgatta tcagatgatt gattggaggt caaggtatgg tatattgtcc gaaatgtgga
     4201 ataaaaaatg aagatgatgc aactttttgt aaaagctgtg gtgcttcgct gaccggttca
     4261 cgacaggagt ctgaccggca tcgggatcaa cgatgcgagg aagattgcgc cggtggacat
     4321 ggtggtcgtg gatgggctat gttctggggc gtcattattg tcctcattgg tcttgtgatt
     4381 ctgtttgaag tggtcattaa agatatggcg aaaacctatt cgtggctctc atgggtcaac
     4441 acggtacaat ggaactggat tttcgcattt gtcattgcga ttttcattat catctttggt
     4501 cttcgtgtac tctctcgccg ttaaacagac gccgtgaaca ttttgcagga ttcaacacaa
     4561 ttcctacaga gggatctatg atgcgatttt gagagttgta ctctcgtatc aagccaaagg
     4621 aaacagagaa gtattgagaa aagacaatag ataaattctc tggtggctcc accgacagcg
     4681 atggtgatac taagaaagct gagcggtaag agactgttga taaaaacatg tacagtcgca
     4741 cgtttactcc gatgaagtat ttcaatgtac accacagcaa gaatgaacaa accaatgaga
     4801 agcaaggcta gagatatcat tcttgtaggt tggaaatcag tcctcaaata tagaatcatt
     4861 aagatgattg atgcacataa accaatgaaa agacctaagc atcctgcaca ccaggttttc
     4921 tttctccaat aaatcgtatg attctgaaag gctagacaat ccgggtgatg acctcgaaaa
     4981 aatcgttgcc tcagagattg tgtatctttt tcttcgttct taaccttatg aaaataccgt
     5041 cgtgtccaat tgggccggac tgtaaaagaa atcccgatga cacaacacaa gatgaatacg
     5101 ctggcaattc ctatcttctc cgatacggag agcggagatg cttgtgaaga tatcgttcct
     5161 actataaaaa tcagaagtac cgctgcgata gaagaaaacg caatatacag aggaatgatt
     5221 cttatgtgct tcaaattaat ctcctgaatt tctcaaggac gaactgaccg gccatatgct
     5281 gatacattgt ccgtttcagc ttatttttgg ctaacacccc aatgatgctt cgtgtcagcg
     5341 acccaaaaaa cattcgataa caacgccaga gttcctcctg aacttctttt cgagttaagg
     5401 tttcggttgg catgatcgca tgtgccatat cataattggc ataatttgta tcctgtatcc
     5461 aaccgttttt cttcgcagtc tcataataga ttgttccagg aaacggagtg agagcggtat
     5521 aaatcgctat atcaggggaa agatccactg aatactgtcg gagttgttcg atagattcat
     5581 gggtatcttt acgtcccccg atcacaaaca tcgcctgaga gaaaatatct ttttgcttca
     5641 gtattttcat cgcctgatac gcatcttgtg ttttgattcc tttcttatat tcatgtaaca
     5701 tctccggtga attcgtttcg acaccacata gaatccaata ggtgcccacc tcacggaggt
     5761 ttccaatcag atcaggatgt tgcgcgatat catcgattcg tgcctggaag aaaagcatga
     5821 tatcatcctt gcatgatttc tgtcggagct cttcgtatag ttgcttccct cgatgactca
     5881 gtctcagatt atcatctgcc agccagagga acacaccccc gtaggtgtca ttgagatatt
     5941 caatttcatc agcgatcctt ttcgctgact tggtcctcca tctgctcccc caatgtttcc
     6001 attgggtaca aaaggaacat tgatgttcgc atcctcgtgc gccttctaaa atcatgtacc
     6061 ttgtgttccg ccctgccatc atggtgaaat gatatcgttt gaggttcttt tctacgagat
     6121 gatacgcagg atagggaagc gtgtcaagat tttcaattaa cggacgtgtc ggattatgaa
     6181 caatggtatt attttttcga tacgaaatcc cttgtacatc atttattttt cctttgtttt
     6241 gcaatgtttt aataagatca acgactgttt gttcaccttc acctctcacg atgtagtcga
     6301 tctctggata gttacgaaga gattcgtcag tggcaaacga aaaatgaata cctccgacga
     6361 ttgttgcaat atcctcgttt acggtttttg ctatctcaca ggttcttgca caggtgtacg
     6421 cgtttgtggt aaagccagag gtaagaacaa tggatggatc tgtcgattca atgtatttct
     6481 caagaccttt ccagtcgagt cgatctgctt ggcagtctac gatatcgatg tcgattcctg
     6541 ggagttctct ttctacatag gctgcaagga ttagcaagga ggatggtggg gggaggtact
     6601 cacccatgac aaaccagaaa ttccgtggtg gttcaacaaa caagagtgac gatttcatct
     6661 gcttcaatta ggaaacatgc aatatctaca aataaattat tctctttacg atattctttt
     6721 gaatagtatt aaaaaaaata aagggaagat gagggtttag aagaacttcc acacctcgat
     6781 ttccttggag tcaacgtttc cttctttatc aaacgctttg acggtgaggg tttgtctgaa
     6841 gaatgctggc tgactccacg accaactgta cggggcggtc gtatcgtttc caagcaactg
     6901 gaggttgacg tagaattcca cacggtcaat gcctgagtca tcatctgttg cattcacgtt
     6961 tacatcaatt tttccaatta tgagtgttcc tcctgaaagc ggcatgattt ctttattcag
     7021 gaaatacagc gcatattctg gtttggtgat ctgaagactc ggtggcgtgg tatccgggac
     7081 cgggcaaata accacagcct gctcaacaac cgtatagtta tacccagcat cataatcagt
     7141 gagccaccat tgtgtgttga tatcgggttg tggagcatca acagagacgt tgtagatatc
     7201 atgagagaca tacgcggcat cattgtgatc gtttggtgcg gggttggcga tatcaacgat
     7261 ggggtcgctg aacgcaatca tggaatcttc tgagttgact cccgcgcacg tcacgtagtg
     7321 tccccctttt ctttcagagt gtggtggagg attataatat tctgttttaa acgcccaatc
     7381 tattgatgat aggacgttat ctttccatgc ttgacccggt ggataggggt tgccaacatc
     7441 gtaactccaa tagtaatgtg ggtcttccac aagctgacga acatcaaaat agtaggttgc
     7501 acctggtgta agagatatgg agggattgaa atcgaaccga atccatgttt gaccagggag
     7561 aaatcctggg ttgaccgtgg attttccaag gagatttccc ggacgattat catagacatt
     7621 cacctcgaca tcagtcggag ggaacgtgct gacgctctga agaagaattt caatggcatc
     7681 aagccgtgtc accgttggaa taaaaccttg agaatcccat aaagtattga tttgtaaaac
     7741 tttcaggacc ggtcctaaaa gctgcatttg atcaaccatc aacggtccta tgatatagtt
     7801 ataatctcca agtaagagaa tgacatcttg actgcgttca atttcacctt ctataaagga
     7861 aaaggttggc cggtcgtagg tctgcactgt gaatttatct gtcagtccgg ttgtggtaaa
     7921 ccaatcacta atcgctgaat gcatatcatc gacataggtt gttcctttct ctgtggtgtt
     7981 catcgctctc gcaagatttt gaataagtaa tgggacgttg gttgtcgtat gatcatcacc
     8041 aattccataa tcctgaacta atgcaaactg atcctggccg tcaccagggg taccttctgg
     8101 gtctgcgaat tttgagtcaa accaccagaa acaattcgca acagcaaccg ggccgcaaaa
     8161 cgcccattct acaacatcat caccaaccgc tgtgctatta agagcacaat caggaccagg
     8221 agcgatacag ttttctgatg gattgtagat gtcatcgcct gcgaccgttg aatcaatcat
     8281 attatttggt ccaggagcta tctttttcca ctgatcttgt tgttgactga aatcggggag
     8341 tccctgcggt acatagttag gatatgatgg tttccagtac catcccctgc tctccatttt
     8401 tttattgttt ttcgaaacga gacctgtgtc tgttgcttgt actgaggtta ttaacagaac
     8461 gaacaacacg aaaaaagaca ccgcagcttt tccaaggtta ttctttttca tcttttttcc
     8521 ctccaatcca ttttaacgat atatagttcg ctgggatgcg agaagatttc ctcctattct
     8581 cgaatcctcc ccttttctat atttgttaac aataaatt
//
LOCUS       DATMXJ010000055         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_1067, whole genome shotgun sequence.
ACCESSION   DATMXJ010000055 DATMXJ010000000
VERSION     DATMXJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..814
                     /locus_tag="VN377_01650"
     CDS             71..814
                     /locus_tag="VN377_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR27120.1"
                     /translation="MKKEPQNRFSNVYEDSERAEAYSKLKFDKTYHLAYRDLPIIISN
                     HTHGNNALDFGCGTGRSTRFLQTLGYKTIGIDISEEMITIARQLDPAGEYHRINDGDF
                     SSLPSSSFELILSAFTFDNIPTMEQKIRLFKGLSMLLTQNGTIINLVSSPEIYTHEWA
                     SFTTKDFPENATAKSGDIVRIITTDIADTRPCYDIYWSAEDYKTVYSKADLHIIKTYK
                     PLATGEEPYQWINETRIAPWTIYVLKKKT"
     gene            complement(848..1642)
                     /locus_tag="VN377_01655"
     CDS             complement(848..1642)
                     /locus_tag="VN377_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWR27121.1"
                     /translation="MDKIGKYEVSAFTKARQDITIVSQEGKRRLVIHALLEIDVTNAR
                     GMIAALKGKQDISFTGWVVKCVAQAVSEHKQMNSYRLGRKRIVSFEDVDIPTPIERNV
                     GGEQRPLAYIIRKANEKTVAEITKEIRTVQHQVIDPSTEVLGADLSGFERWAITAPGW
                     LKKLGVRVLRRRGLSKKKHFGTVGVTAIGMKGCFPGWAIGMGGPIATLIIVGGITKKP
                     GVVDDSIQIREILHVTVTADHSVVDGSPLVRFISRLTELLETGYGL"
     gene            1725..2300
                     /locus_tag="VN377_01660"
     CDS             1725..2300
                     /locus_tag="VN377_01660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWR27122.1"
                     /translation="MALKKRPVLIIEHCEPKLSEWLLLEYKHAAKLWNGNLMFTKVTD
                     AKTAKILRNLGVVKKENAKDLYSDKNCIVLDPQAQRPLTPKDCTNADALIVGGLLGYE
                     KPQGRTKTLISASSGFHTRHLGPLQLSIDGAVFVIKAITLGLTLKDIEIAKEIEIIHD
                     TVHSTILPFGYPIIENKPVITPGLIEYLSKL"
     gene            2404..3183
                     /locus_tag="VN377_01665"
     CDS             2404..3183
                     /locus_tag="VN377_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWR27123.1"
                     /translation="MQNKKKIFSIVLVLAMVIGTVALSGCTQETKNKIIVGTSADFAP
                     FEYKAANQTIIGFDIDLIKKILTDQGYTVEVQDIGFDSLIPALQTGKIDVIAAAMTID
                     DERKQQVDFSIPYYDSNQTVLVKVGANVSITTIEDLKNYTVGAQTGTTGWAWVNDTLV
                     ATGKLNSDNFRSYDLYTDAVADLIIGPSRVGAVVIDTPVAKAFVKSGNVKIVLNITTN
                     ESYGFAVEKGNTELANKINTGLTAVMATTYWETLVEKYFAS"
     gene            3203..3877
                     /locus_tag="VN377_01670"
     CDS             3203..3877
                     /locus_tag="VN377_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWR27124.1"
                     /translation="MALEALIESLPSLLDGLIVTLLLTAFGILLGTILGIVLATGRSY
                     AKRPIAYSILIYEKVLRGIPLLVLLFLIYFGLPQIGINMDAFTAAVIGLGLRSSAYQA
                     QIYRGAINSIPEGQIHAAFSLGMNKLKAIRHVIIPQMLRLSIPGWSNEFTILLKDTSL
                     AFGIGVIELMRQGRYIQVRDPDSVLLVFLLIALIYFVLVFTANKTMRYVEKKYQMAGY
                     QTEAHR"
BASE COUNT         1226 a         1024 c          759 g          987 t
ORIGIN      
        1 gcctttcacg ggtcagagac atcacaacaa taaatttttc taagctcaga ccatatatct
       61 cttattgacc atgaaaaaag aacctcaaaa cagattttcc aatgtctatg aagattcaga
      121 acgtgcagaa gcatactcaa aactcaaatt tgacaaaaca tatcacttag cctaccgaga
      181 cctccccata ataatatcca accataccca tgggaacaac gctttggatt tcggctgtgg
      241 cacaggacga tccacacggt tcctccaaac actcggatac aaaaccatag gaatcgacat
      301 ctcagaagaa atgatcacca tcgcacgaca actcgacccc gctggagaat atcaccgaat
      361 caacgacggc gacttcagca gtctaccctc ctcatccttt gaacttatcc tttccgcgtt
      421 caccttcgat aacatcccga ccatggaaca gaaaatcaga ctcttcaaag gtttatcaat
      481 gcttttaaca caaaatggga cgatcatcaa cctcgtctca tcaccggaga tctacaccca
      541 tgaatgggca tccttcacca caaaggattt ccctgagaac gcgaccgcaa aaagtggtga
      601 tatcgtccgc atcatcacca ccgacatcgc agatacacgc ccctgttatg acatctactg
      661 gtcagcagaa gactacaaga cagtgtattc gaaagccgac ctgcacatca tcaaaacgta
      721 taaaccattg gcgacaggag aagaacccta tcaatggatc aatgaaacaa ggatagcacc
      781 atggaccatc tatgtcctaa aaaagaagac atagcgcttt tctccatcga tgaaaaacct
      841 gcacctttta taatccgtac cctgtctcca acagctcagt caaccgagag atgaaacgca
      901 ccaacggact cccgtcgacg acgctatgat cggcagtgac agtaacgtgg agaatctccc
      961 ggatctggat tgaatcatcc acaacccctg gtttcttcgt aatgcccccg acgataatca
     1021 atgtcgcaat cggaccaccc atcccgatcg cccagccagg aaaacagcct ttcatcccaa
     1081 tcgcggtgac cccaacagtc ccgaaatgct tctttttcga caaccctcga cgccgaagca
     1141 cacgaacacc gagttttttc aaccagcctg gcgcagtaat tgcccaacgc tcaaaccctg
     1201 aaagatccgc ccccagcact tctgtggatg gatcaatcac ctgatgttgc accgtgcgga
     1261 tctcctttgt tatctcagca acggtttttt cattggcttt ccggatgatg tacgcaagcg
     1321 gccgctgctc accaccgaca ttccgttcta ttggggtagg gatatcgaca tcttcgaagg
     1381 acacgatcct cttccgaccc aatcgatagg agttcatctg tttatgctca gagaccgcct
     1441 gagcgacgca tttcacgacc cagcccgtaa aagaaatatc ctgttttcct ttcaacgcgg
     1501 caatcatccc acgagcgttc gtcacatcga tctccagcaa cgcatggata accaatcgtc
     1561 gttttccttc ctgagaaaca atggtaatat cctgccgtgc tttcgtaaac gctgagacct
     1621 catacttgcc gatcttatcc atacaaccac accgtcgata cttctccaat tcaaatactt
     1681 tttcgcagga actcttatta ccatcgacat ccgtacacct tcctatggct ttgaaaaaac
     1741 gaccagtact catcatagaa cactgcgagc caaaactttc agaatggctc cttcttgaat
     1801 acaaacacgc agcaaaactc tggaacggaa acctcatgtt caccaaagtg actgatgcca
     1861 aaacagcaaa aatcctacga aaccttgggg tggtgaaaaa agaaaacgca aaagacctgt
     1921 attcagacaa aaactgcatt gtccttgacc cccaggcaca acgaccactc acacctaaag
     1981 attgtacaaa tgcagatgca ctcatcgtcg gtggactgct tggctacgaa aaaccacaag
     2041 gacggacaaa gacactcatc agtgcctcct cagggttcca cacacgacac ctcgggccac
     2101 tccagctttc catcgatgga gcagtatttg tcataaaagc tatcacccta ggcctcactc
     2161 ttaaggatat cgaaatcgca aaagaaatag aaatcatcca cgacacggtt cactcaacga
     2221 tactcccctt tggatacccg atcattgaaa ataaaccggt catcaccccc ggtcttattg
     2281 aatatctctc caaactataa actcagttgg tagtattttt tccctctttc atctttgtaa
     2341 ttactacaat gtttaaataa tactccactg attatctcca cgaacctaag ggtgaggtaa
     2401 attatgcaaa acaaaaagaa aatattttca atagtcctgg tacttgcgat ggtcatcggc
     2461 actgttgcat tatcaggatg tacgcaggaa acaaaaaata aaatcattgt aggaacatca
     2521 gcggattttg cgccttttga atataaagcg gctaaccaaa ccattatcgg ttttgatatt
     2581 gatctgatta aaaaaattct cactgaccag gggtacacag ttgaagtcca agacatcgga
     2641 tttgattcct taataccagc actgcaaaca ggtaaaatcg atgtcatcgc cgctgcaatg
     2701 acaatcgatg atgaacgtaa gcaacaggtt gactttagca tcccgtatta tgattcaaac
     2761 caaacagtac tcgtgaaagt gggtgctaac gtaagtatta caactatcga agatttgaaa
     2821 aattatacgg ttggtgctca aaccggaaca actggatggg cctgggtgaa tgatacgctt
     2881 gtagcaacag ggaaattaaa cagcgataat ttcagaagct atgatctcta tacggacgcg
     2941 gtcgctgatt tgatcattgg cccctctcga gtcggtgcgg ttgtcatcga tacccctgta
     3001 gcaaaagcat ttgtaaagag tggaaatgta aaaatcgtac tcaacatcac gacaaatgaa
     3061 tcatacgggt ttgccgtgga gaaaggaaat acagagctgg cgaataaaat taacacagga
     3121 ttaacagcag tgatggcgac aacctattgg gaaacccttg tcgagaaata ctttgcttca
     3181 taaggggaca tagggtgatc tgttggcact tgaagcgcta atagaatcac tcccctcact
     3241 cttagatgga cttatcgtca ccctacttct caccgcattt ggtatcttat taggaactat
     3301 ccttggaatt gtccttgcaa ctggccgctc ctatgcaaaa cgaccgattg cttattctat
     3361 tttaatctat gagaaagtcc tccgcgggat acctcttctg gtgctgcttt ttcttatcta
     3421 ttttgggctt ccccaaattg gaatcaacat ggatgcattt actgctgcag tcatcggcct
     3481 tggacttcga agttcagcct atcaagcaca gatctatcgc ggagcaatca attctatccc
     3541 tgaaggtcaa atccatgcgg cattctccct tggaatgaat aaactcaagg cgattcgaca
     3601 cgtcatcata cctcagatgc ttcgattatc gattcctgga tggtcgaacg aattcacgat
     3661 tcttctaaag gacacctcgc ttgcgttcgg aattggtgta attgaattaa tgcgccaagg
     3721 acgttacatc caagtgagag atcctgactc ggtccttctt gtgtttttac ttatcgcact
     3781 tatttacttt gtactcgtat ttacagcaaa taaaaccatg cggtacgttg aaaaaaaata
     3841 tcagatggca ggataccaaa cagaggcaca ccgatgaaaa taaggaggaa ccatctatgg
     3901 caatgttaga ggtaaaaaat attcacaaat catacggaac tgtcgagatc ctgaaaggtg
     3961 tttcattcac tatggaacaa gaggagacaa aagtca
//
LOCUS       DATMXJ010000056         4691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_6299, whole genome shotgun sequence.
ACCESSION   DATMXJ010000056 DATMXJ010000000
VERSION     DATMXJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6299"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="VN377_01675"
     CDS             <1..2302
                     /locus_tag="VN377_01675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWR27125.1"
                     /translation="DAEYYRRITQISFIELFNKGHIYKGEFPVNWCPRCMTAMADAEV
                     VYSDRTTKLNTIKFYFTKSQDEKLLKFHGIGKDSQGVYVEIATTRPEMLAACQIVAVH
                     PTDERAPWLVGQAVKVPTVDKEVKIVEDDAVDPEFGTGLVMICTVGDKEDLNWVFKYK
                     LPLEMSIDEEGRMTAITGKYKGMKIEDARKTIIQDMTHDELLLKQEPLAQNVGVCWRC
                     KTPVEFINAKQWFLKTIPFKQLVLDTSDAMHWYPEFMKIRLEDWVHSLEWDWVISRQR
                     YFATPIPLWECESCGEVVLARPEDCYIDPTVDKPPVEKCQKCGGRLKGCEDVFDTWMD
                     SSISPLYNTFWHRDEKKFKHLYPMSLRAQAHDIIRTWAFYTILRCTLLTDEKPFESIM
                     MGGFILSEDGTPMHASLGNVIDPLQVIDEYGTDSFRCYAASCALGEDNPFRRKDVVRG
                     TKLLRKLWNVQQFIGNIIKEGEPKKPKKLQDIDQWILTKYSKLVTSCTKQMDVFDYSP
                     AMKDIEYFLWHELADHYLEMIKGSLYNKENVESIQYTLYTVGLGVLKLFAPFFPHITE
                     EIYHDFYKGYEGDDSIHLAAWPKAVLINDEKEQAGETVKEYIAKVRAWKSEQGIALNA
                     PIPALATYASSESITRLQGNGPIIFSTLRYPPTHTFIPGKPEIQETITAVEPVFAKLG
                     PTFKNESSTIVQWIKTHQEELITKIENGKDILISEIPGIKTKTKEALYKSGYLKVKRE
                     VGLKGKKGSTIISFDEYYIELERKAP"
     gene            2465..3055
                     /locus_tag="VN377_01680"
     CDS             2465..3055
                     /locus_tag="VN377_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HWR27126.1"
                     /translation="MTEKNNVLANPAPLGLLGFGLTTVLLNIHNTGAYGVSAMILGMG
                     IFYGGLAQIIAGIMEFKKGNTFGTTAFTSYGLFWVSLVALWVFPSLTFISAGSKAGAT
                     EAGFMAWYFFMWGLFTFCMFIGTLKKNTGLQFIFGSLFILFFLLALRDWGYISGAWIG
                     YEGIICGFSAFYVAIAEVLNETYGKTILPLGKPIIK"
     gene            complement(3058..4044)
                     /locus_tag="VN377_01685"
     CDS             complement(3058..4044)
                     /locus_tag="VN377_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWR27127.1"
                     /translation="MTKVLFFTDITKLPEALSFFPLDSFAGKKVPVKLHMGEKKNPYF
                     VKPDLVKRVVDELKKAKAEPFLFDTTVAYHGSRHTKKDYQILADHHGFTMTNVGCTVV
                     IDDTGVPTVVEGRTYEVADHLRSATHIFALSHVKGHIQSGMGGAIKNFGMGGVTKETK
                     VKIHDGSSPVYQKDSCTYCGVCAEVCPFHAIRVTDDSWHYNEQKCFGCGVCVDACKNK
                     ALKNEDADMQFLLACSAKACVQDKNVLYFNELKRIAKGCDCDPNAGPIICPDIGFLVS
                     DDPVAIDKASLDLINEIKENVFLKINKVDPLKQIKYGEEIGLGSSSYELIEL"
     gene            complement(4041..>4691)
                     /locus_tag="VN377_01690"
     CDS             complement(4041..>4691)
                     /locus_tag="VN377_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hexulose-6-phosphate
                     synthase/ribonuclease regulator"
                     /protein_id="HWR27128.1"
                     /translation="ATTVLFKKYDTTELKEAFMMVSTPNISDAMHKQGALQGIRPIRP
                     GFHMVGQALTVRTIDGDWAKPIEAIDKAEPGSILVVDVNQGRTAVWGELATWSAKLRG
                     LAGVVIDGAVRDYDDLVKIKFPIFSRYIVPNAGEPKGLGEIGAEITCGNQTIRTSDWI
                     IGDDSGVVVVPQDIAQEIANRALDVKEQENRIREEIKNGGSLGSVLKLKKWEKKIG"
BASE COUNT         1276 a         1082 c         1077 g         1256 t
ORIGIN      
        1 cgacgcagag tactaccgca ggatcaccca gatctcattc atcgaactgt tcaacaaagg
       61 ccacatctac aaaggagaat tccctgtgaa ctggtgcccc cggtgtatga ccgcgatggc
      121 agatgcagag gtcgtctata gcgaccggac cacgaaactg aacaccatca aattctattt
      181 tacaaaatcc caagatgaga aactcctgaa attccatggc atcggaaagg attcacaagg
      241 cgtgtacgta gagattgcga ccacccggcc tgagatgctt gctgcttgtc agattgtcgc
      301 ggtgcacccg accgatgaac gagcgccctg gcttgtcggt caagccgtga aagtccccac
      361 cgtcgacaaa gaagtaaaaa tcgttgaaga cgatgcggtt gaccctgagt tcggcacagg
      421 tctagtgatg atctgtacgg tcggtgacaa ggaagacctc aactgggttt tcaaatataa
      481 actcccgctg gaaatgagca tcgacgaaga aggaagaatg acggcaatca cagggaaata
      541 taagggaatg aagattgagg atgcacgaaa aacaatcatt caggatatga cacacgacga
      601 gttgctacta aaacaagagc cgctcgcgca gaacgttggt gtctgctggc ggtgcaagac
      661 gccagtcgag ttcattaacg cgaaacaatg gtttttgaaa accatcccgt tcaaacaatt
      721 ggttcttgat acaagcgacg cgatgcactg gtacccagag tttatgaaaa tacggctcga
      781 agactgggtt cattcccttg aatgggactg ggtgatcagc aggcaacggt actttgcaac
      841 accgataccg ctctgggaat gtgaatcctg cggtgaggtc gtcctggcac ggcctgagga
      901 ttgctatatt gaccccaccg ttgacaaacc accggtcgag aaatgtcaga aatgcggtgg
      961 gcgtctgaaa ggctgcgagg atgtctttga cacctggatg gattcctcga tctccccgtt
     1021 atataacacg ttctggcatc gtgatgagaa gaaattcaag cacctttatc cgatgtcgct
     1081 ccgtgctcag gcacatgata tcatccgcac ctgggcgttt tacaccatcc ttcgctgcac
     1141 gctgctgact gatgaaaaac cctttgagag catcatgatg ggcggattca ttctttcaga
     1201 agacggaaca cccatgcatg ccagccttgg aaacgtcata gaccccctgc aagtcattga
     1261 tgaatacgga actgattcct tccgttgtta tgcagcgagt tgcgccttgg gggaggacaa
     1321 cccgttccga agaaaagacg tggtccgagg gaccaaactg ctgcggaaac tctggaacgt
     1381 gcagcaattc atcggcaaca tcatcaaaga aggagaacca aagaaaccaa agaaactcca
     1441 agacatcgac cagtggattc tcaccaaata cagcaaactt gtcaccagct gcaccaagca
     1501 gatggatgtg tttgattact cgcccgcgat gaaagacatt gaatatttcc tctggcatga
     1561 gcttgcggat cattaccttg agatgatcaa agggtcactg tataataagg aaaatgtaga
     1621 aagcatccaa tacacgttat atacggtcgg attaggtgtt ttgaaattat tcgccccgtt
     1681 cttcccgcac atcaccgaag aaatctatca tgatttttac aaaggctatg agggagacga
     1741 cagtattcat ctcgctgcat ggccaaaggc tgtcctcatc aatgatgaaa aagaacaagc
     1801 tggggagacc gtcaaggagt acatcgcgaa ggtccgggca tggaaaagcg aacaaggaat
     1861 cgctcttaat gcaccgatac ctgcacttgc aacctatgcg tcttcagaaa gcataacaag
     1921 actgcaagga aatggaccca ttattttttc caccctgcgg tacccaccga ctcatacatt
     1981 catcccagga aaaccagaga tccaggagac catcactgca gtagaacccg tgtttgccaa
     2041 acttggtcca acgttcaaga atgaaagttc caccattgtc caatggataa agacacatca
     2101 agaagaactg ataacaaaga tagagaacgg aaaagacatc cttatctcag agatccccgg
     2161 gattaaaaca aaaacgaagg aagcgttata taaatccggg tacctcaaag taaaacgtga
     2221 ggtaggcttg aaaggtaaaa aaggaagcac gatcatatca tttgatgagt attatattga
     2281 acttgagagg aaggccccat gagaataaca agaaaaattt tacgaggctg gatggtcaaa
     2341 atcatcgcga ttctgattgt ccttggcatg atcttcacag ggttcgtagt aatcttttgg
     2401 aaatagttgt ttttctcgat agatttttaa acaactgttt tattcagccg gacagtgaaa
     2461 acatatgact gaaaagaata atgtgttagc aaatcccgca ccattaggtt tactaggctt
     2521 tggactgaca actgttcttc taaatatcca taataccggg gcatatggag taagtgcaat
     2581 gattcttggg atgggtattt tctatggcgg gcttgcccag atcatcgcag ggattatgga
     2641 attcaagaaa ggcaacactt ttggaacaac tgcttttacc tcatatggtc tgttctgggt
     2701 ttctttggtt gcattatggg tatttcccag tctgacgttt atcagtgctg gttcgaaagc
     2761 aggagcaacg gaagcaggat tcatggcatg gtatttcttc atgtggggat tgttcacctt
     2821 ctgtatgttc atcgggacat taaaaaagaa cacaggcctt cagttcatct ttggatccct
     2881 tttcatccta tttttcttac ttgctcttag agattgggga tatatcagtg gcgcatggat
     2941 tggttatgag ggaatcatct gtggtttcag cgctttctat gtcgcaattg cagaagtgct
     3001 gaatgaaacc tatgggaaaa caatccttcc gttaggaaaa ccgatcatta aataactcta
     3061 cagttcgatc agctcatatg aggacgatcc caacccgatc tcctccccat attttatttg
     3121 tttcaatgga tcgaccttat tgatttttag aaacacgttt tctttgattt cattgataag
     3181 gtctaatgat gctttatcga tcgccaccgg atcatcgctc accagaaatc cgatgtcagg
     3241 gcagatgatt ggtcctgcat tagggtcgca atcgcagcct tttgctatcc gttttaattc
     3301 gttgaaatat aatacgtttt tatcttggac acatgctttt gcagagcaag caaggagaaa
     3361 ctgcatatct gcatcctcgt ttttgagcgc cttgttttta caggcgtcaa cacagacacc
     3421 acacccaaaa cacttctgtt cattgtagtg ccatgaatca tcggtgaccc tgatggcgtg
     3481 aaacgggcag acttcagcac acaccccgca ataggtacat gaatccttct gatataccgg
     3541 gctgctccca tcatggattt ttacttttgt ctcttttgtt accccgccca ttccaaagtt
     3601 ctttatcgca ccacccatcc ctgattggat atgtcctttc acatgggaga gtgcaaaaat
     3661 atgggtcgca gatctgaggt gatcagcgac ctcgtaggtc ctcccttcaa caacagtcgg
     3721 tacacctgtg tcatcgatga ctacggtaca accaacattt gtcatcgtaa atccatggtg
     3781 gtctgcaagt atttgatagt ctttttttgt atgtcgagat ccatggtagg caaccgtggt
     3841 gtcaaaaaga aatggttctg ctttggcttt tttaagttca tcgaccaccc ttttcactag
     3901 gtctggtttg acgaaatacg ggtttttctt ctcccccata tggagtttca ctggtacttt
     3961 ttttcccgcg aaactatcga gtggaaagaa agaaagcgct tctgggagtt ttgtgatatc
     4021 tgtaaagaac agtactttgg tcatccgatt tttttctccc attttttgag ctttaaaaca
     4081 ctgccaagtg atccaccgtt tttgatttct tcgcggatgc ggttctcctg ttctttgaca
     4141 tcgagtgcgc ggttagcgat ctcctgtgcg atgtcttgtg gtacgacgac cactcctgaa
     4201 tcatcgccga tgatccaatc actggtccgt atggtttggt tcccgcaggt tatttcagca
     4261 ccgatttccc cgagtccttt tggctcccct gcgtttggga cgatatagcg ggagaagatt
     4321 gggaatttga tttttactaa atcatcgtaa tcgcggaccg ccccgtcgat gacgacgcca
     4381 gcaagacctc gaagctttgc gctccaggtc gcaagttctc cccataccgc tgttctgcct
     4441 tggttcacat caacgacgag gatacttcct ggctctgctt tatcgattgc ttcaatgggt
     4501 tttgcccagt caccatcgat ggttcgtacg gtgagagcct gaccgaccat atgaaatcca
     4561 gggcggatcg gtcgtatccc ttgtaacgct ccttgtttat gcatcgcatc agaaatgttc
     4621 ggcgtcgaaa ccatcatgaa tgcttctttc agttcagtcg tatcatattt tttgaacaaa
     4681 acggttgttg c
//
LOCUS       DATMXJ010000057         4866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_43016, whole genome shotgun sequence.
ACCESSION   DATMXJ010000057 DATMXJ010000000
VERSION     DATMXJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43016"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VN377_01695"
     CDS             <1..336
                     /locus_tag="VN377_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27129.1"
                     /translation="VLQTVEVIHADAKDAEKIIPTKADQIIMNLPFSAYKFFSVALSI
                     AAPTCKVHYYDIIGEEEIEGRISSLKKTAKRQGYILTEVLIHRIKSYAPREFYIGLDI
                     TATKHADVA"
     gene            322..395
                     /locus_tag="VN377_01700"
     tRNA            322..395
                     /locus_tag="VN377_01700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Thr,seq:tgt)
     gene            518..1384
                     /locus_tag="VN377_01705"
     CDS             518..1384
                     /locus_tag="VN377_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWR27130.1"
                     /translation="MTDHCSDCGAELIEGSKFCTVCGKTVEQQQVTPVQSQPQQQEEH
                     HQGSVVPTKKQKPRKKLVIGLLAIAAVVIIILIIIVYLQSGTNFLGNGNADSRFIGEW
                     EDNTFGSPYQWKFNSDSTLEKGLSADSMNDTGTWNVTGNQLCLYDNAVCYTYVISNDG
                     TVVTLKRIGDSVIYPANLILTKKGQQGTNRTPAIDCTVDSSINRIIIESIDANVRWSD
                     IKITTTPATANWQVQDGSQKGLARIGITDTITTFVTVGDNIFVLDPVGDVSVTLTYLP
                     TNAILGNWTVNI"
     gene            complement(1395..>4866)
                     /locus_tag="VN377_01710"
     CDS             complement(1395..>4866)
                     /locus_tag="VN377_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27131.1"
                     /translation="LLAEPNNEKLTIQDTMVNKDDDSLQESYNLQYTIYFSETDLNFG
                     TFQGYDFITMKECSYLTEYGKPLLPVKHLLIALPNDMSATNIQIISLQKQSLQGWYTL
                     YPAQRPQKIGEISDSTLDTPLTRATYMSPFPYPERLVALGPQTDLAGQSMVDLTIYPL
                     QYLPLQKKLNLITSITLTIEGSSGYICGDYLSEHISDTSRDMYQQMIQDMVVNPENVA
                     LQSSPSPQPLGVGPGDYDYVIITQSSWVSAFQPLADWKTQKGVPAAIVTTDWIYNSGG
                     YSGSNVQKIKAFVQDAYTVWGTIYVLLGGDINVVPCYYKTFSDVDPDPVPNDVYYADF
                     DSDWICEVNIGRASVTGPGSGTGQIGTFINKIMTYETNPPLTNYAKKAGFFGFDLDDI
                     TDAEQCKITIKNTFIPASWTMTTVYDSQGGNHFTNVINALNAGQNFVNHADHSNNDCM
                     GTGYVNHDLLMYSADMDALTNANKQTIFYSMGCDPAAFDVSNCIAEHFVRNSNGGGIA
                     FIGNSRYGWYEWATYDTLSMGFDIHFFQSMFQENLYHLGVAFSDHKNDGYQQAPGDEY
                     YQYIFTELTLLGDPELPVWTENPSAFVVSHPSTIPLSSSSFTVHVQTTGGSNVQNAYV
                     CLWKGDEIYERGYTNSAGNAPFTVSPETGGSMQVTVTKQNYLPSETTAQVIENNLPPY
                     QPSSPNPTNGATNIPINSDLSWIGGDPNPGDTVTYDVYFGTSSNPPKVGYNQSAVSYD
                     PGTLNYVATYYWKIIAWDSYGESTAGSLWVFSTKPNSPPVFGSPSPANGSIGNPLSFT
                     WSISINDPDSSIFSWSIQCSNGQTNSGTGATNGTKTLALAGLSLSTTYTVWVNATDPG
                     GSGLYTRKWYIFTTLSDSVPPTTTVYFTGTLGDNSWYVSPVMITLIATDDMTGVDYTM
                     YNLDSASWNIYTSPFTVSDDATHTIEYYSVDNNGNIEPVKSADFKIDQKPPMTTHTFS
                     GDEGKNGWYLTLNIILNAVDNTSEVDHTYIKIDNSVWTEYTVPIVFTSDGIHTFEYYS
                     VDNAGNEEPVKGPFTIKLDPTPPMITLTKQQIDLFTIKFIAEVSDGVSGVDYVEFFVD
                     GDLQNNDTITPYEWIWNGFGDYTVTATVYDKAGNSKSQSMSTPNGYNIQTLFNPYQIQ
                     DVAVKYQIRA"
BASE COUNT         1358 a          970 c         1103 g         1435 t
ORIGIN      
        1 gtcctacaga ccgttgaagt catccatgct gatgcgaaag acgcggaaaa aataattcca
       61 acaaaagctg atcagatcat catgaacctt ccattttctg catacaagtt tttttcagtt
      121 gcgttgtcca tcgcagcacc aacatgtaaa gtacattatt atgatattat aggcgaggaa
      181 gagatcgaag gaaggatttc ttctttgaaa aaaaccgcaa agagacaggg ttatatctta
      241 actgaagtat tgattcacag aattaaatcg tacgcaccaa gagaatttta tataggatta
      301 gatattaccg ctacgaaaca cgccgacgta gcttagcccg gttgagcggc tgacttgtaa
      361 tcagcaggtc gggggtccaa atccctccgt cggctttcct gataaaaaaa atatgactaa
      421 cataaaatat tgtgagcatg aaaaatgttc gtttataaag gaatgttttt aaatcacaaa
      481 ttgttatata aattttaaca taggagtggg acaaaatatg acagatcatt gttcggattg
      541 tggagctgaa ctcattgaag gttcgaaatt ctgtacagta tgcggaaaaa ctgttgaaca
      601 acaacaagta actccggtac aatctcagcc gcaacaacaa gaggaacacc atcagggttc
      661 ggtggtgcca acaaaaaaac agaaacccag gaaaaaatta gtcataggtc ttttagcaat
      721 cgctgcagtg gtgatcatca ttttgattat catcgtatac ctccaaagtg gtactaattt
      781 tcttggtaat gggaatgcag acagtcgttt tatcggagaa tgggaagata atacatttgg
      841 gagtccgtac caatggaaat tcaacagtga cagcacatta gagaagggtc tttctgccga
      901 ttcaatgaac gacactggca catggaatgt tacaggtaat caactctgtc tctatgataa
      961 tgcggtgtgc tatacgtatg taatttcaaa cgatggaacc gttgtgacat tgaagaggat
     1021 cggagacagt gtgatttacc ctgcgaatct catattaaca aaaaaaggcc aacaaggaac
     1081 caacagaaca ccagctattg attgtacagt tgattcatct atcaatagaa tcataattga
     1141 gtcgattgat gcaaacgtaa gatggagcga cataaaaatt acgacgacgc ctgctaccgc
     1201 aaactggcag gttcaggacg gtagtcaaaa gggtcttgct agaattggta ttaccgatac
     1261 gattactaca tttgttacgg ttggtgataa tatatttgtt ttagatcctg ttggtgatgt
     1321 ttcagtcaca ttgacgtatt taccaacgaa tgcaatactt ggtaactgga cggtaaacat
     1381 ctaaatcaga tttcttacgc tctaatctga tatttcacgg caacatcctg gatttgatat
     1441 gggttaaaga gcgtctggat attataacca ttaggtgtgc tcatcgattg gctctttgaa
     1501 ttgcctgctt tatcatagac ggttgctgtg acagtataat ccccaaatcc gttccatatc
     1561 cattcatagg gagttatggt atcattattt tgtaaatctc catcgacgaa gaactcaaca
     1621 tagtccacac cgcttacccc gtcgcttacc tctgcaatga atttgatggt gaagagatcg
     1681 atttgttgtt tggtaagagt aatcatcggg ggggtagggt ctagtttgat ggtaaaagga
     1741 ccttttactg gttcctcatt cccagcgttg tcaactgagt aatattcgaa agtatgtatt
     1801 ccatcagaag taaaaacaat gggaactgtg tattctgtcc acacactgtt gtcaattttg
     1861 atataggtat gatcgacttc agaagtatta tcaactgcat ttaggataat attaagtgtg
     1921 agataccaac catttttacc ctcatcacct gagaacgtat gggttgtcat cggtggtttc
     1981 tgatcgattt tgaaatcagc ggatttcact ggctctatgt tcccattgtt gtcaacagag
     2041 taatattcta tggtatgcgt agcatcatct gaaacagtga acgggcttgt atagatattc
     2101 cagctggctg aatcaaggtt gtacatcgtg tagtccacac cagtcatatc atccgttgca
     2161 atcagggtta tcatgactgg gctaacgtac caagagttat ctccaagagt ccctgtgaag
     2221 tacacagtcg ttgtcggtgg tacgctatct gagagtgtgg tgaagatgta ccattttctt
     2281 gtgtacagac cacttccgcc tggatcagtc gcgttcaccc acactgtgta ggtcgttgaa
     2341 aggcttaaac cggcgagggc aagtgtcttt gtcccatttg tcgcaccagt cccactgttt
     2401 gtctgtccgt tgctacactg aatagaccat gagaagatgc tactatccgg atcattgatc
     2461 gagatgctcc aagtaaaact taagggattt ccgattgacc cattcgctgg tgatggtgaa
     2521 ccgaataccg gtggactatt tggcttagtg gagaagaccc ataacgatcc tgctgtcgat
     2581 tccccgtaac tatcccaagc gataatcttc caataatagg tcgccacata gttcagagta
     2641 cctggatcgt aggatactgc cgattgatta tatcctacct ttggaggatt actacttgtg
     2701 ccaaaataga cgtcgtaggt gaccgtatct ccagggtttg gatccccacc gatccagctc
     2761 agatcgctgt tgattggtat atttgtcgca ccattcgtag ggttggggga gctgggttga
     2821 tatggaggta gattattttc aatgacctgt gcggtggtct ccgaaggaag gtagttctgc
     2881 ttagttaccg tcacctgcat acttcctcca gtctctggtg agaccgtgaa aggagcattc
     2941 cctgcggaat tggtgtaacc acgttcgtaa atctcatcac ccttccatag gcacacgtat
     3001 gcattttgca cattgctccc accagttgtt tgaacatgga cggtaaaaga agaagaggaa
     3061 agcgggatcg tggatggatg acttacgacg aatgccgagg gattctcagt ccagaccggt
     3121 agttctggat ccccaagtaa ggtcagttca gtaaagatat actggtaata ttcatccccg
     3181 ggagcttgtt ggtatccgtc attcttatga tctgagaatg caactcccaa atggtaaagg
     3241 ttctcctgaa acattgattg gaagaaatgg atatcaaatc ccatggataa ggtatcatat
     3301 gtagcccatt cgtaccagcc atatcgtgag ttcccgatga aagcgattcc gcccccgttg
     3361 ctgtttcgta cgaaatgttc tgcgatgcaa tttgagacat cgaacgcagc aggatcacaa
     3421 cccatggagt aaaagatagt ctgtttgttt gcattcgtta aagcatccat atcagcacta
     3481 tacataagca agtcatgatt cacatagccg gtccccatgc aatcattgtt cgaatgatcc
     3541 gcatggttca caaaattctg acctgcattg agtgcattga taacgtttgt gaaatggttt
     3601 cccccctggc tatcatagac ggttgtcatg gtccaactag ctggaataaa cgtgttctta
     3661 atggtaattt tacattgttc tgcgtcagta atatcatcga ggtcgaaacc aaagaaccca
     3721 gctttttttg cgtaattcgt caatggcggg tttgtctcgt atgtcatgat cttattgatg
     3781 aaggttccta tctgtccggt gccacttcct ggaccggtga ccgatgctcg tccgatgttg
     3841 acttcgcaga tccagtcgct atcaaaatca gcgtagtaca catcgtttgg gactggatct
     3901 ggatcaacat ctgaaaaggt cttataataa catgggacaa cattgatgtc accacccaat
     3961 agcacataga tcgtccccca gacggtataa gcatcctgta cgaatgcttt gattttttga
     4021 acattcgatc cactataccc acctgagtta taaatccaat ctgttgtcac tatcgcagca
     4081 gggactcctt tctgtgtttt ccagtcggca aggggctgga acgcactgac ccagcttgat
     4141 tgtgtgatga tcacatagtc ataatcacct ggtccaacac caaggggttg aggacttggt
     4201 gaagattgaa gagcaacatt ttctggatta acgaccatgt cttgtatcat ttgttgatac
     4261 atgtcgcggc ttgtgtcaga tatgtgttcg gataaataat caccacaaat atatccgctg
     4321 cttccctcga tggttaaggt aatcgaagtg attagattca attttttttg aagaggaaga
     4381 tattgaagtg gatagatggt caaatcgacc atgctttgac cggcaagatc agtttgcggt
     4441 cctaatgcca caagtcgttc cgggtagggg aatggtgaca tgtacgttgc tctagtaaga
     4501 ggtgtatcaa gagtagaatc tgaaatctct ccgatctttt ggggtcgttg agccggatag
     4561 agagtgtacc atccttgtaa tgattgtttt tgaagggaaa tgatctgaat gtttgttgcg
     4621 ctcatgtcat ttggtaacgc tatcagaagg tgttttactg ggagtagtgg ttttccgtac
     4681 tcggtcagat acgaacattc tttcattgtt atgaaatcgt agccttggaa tgtaccaaag
     4741 ttgaggtctg tctctgagaa atatatggta tattgtaggt tgtagctttc ttgcaggctg
     4801 tcatcatctt tgtttaccat ggtgtcttga atagtgagtt tttcattgtt tggttctgct
     4861 aatagg
//
LOCUS       DATMXJ010000058         6889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_1110, whole genome shotgun sequence.
ACCESSION   DATMXJ010000058 DATMXJ010000000
VERSION     DATMXJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..923
                     /locus_tag="VN377_01715"
     CDS             63..923
                     /locus_tag="VN377_01715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWR27132.1"
                     /translation="MFILGGTSAKNVATNLANRLQQPQVQTTYKRFPDDEFYVRLHDN
                     IAGQDVLIVQTAYPDQKIVELFLLQDAVHEAGAKTITVILPYFGYSRQDKKFEDGEAI
                     SARAIAQHISLHADCVITVDPHKEHILKFFTVPAYCCSAVSSIAQYLKEKNIDFVLAP
                     DKGAKERAKEAAALINCEYDYLEKTRIDGSTVKITPKKLDARGRHVAIIDDIISTGST
                     MAHSIKELKKQGAASVSVACTHGLFVGGAKEKLLAADCDEIISTDTIETEYSKVSAAE
                     CIAETLLKTI"
     gene            1011..5171
                     /locus_tag="VN377_01720"
     CDS             1011..5171
                     /locus_tag="VN377_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HWR27133.1"
                     /translation="MRKRAQFKMPMQSEPVPSSQSTPVTAAPERKRFRVKKNWWMGVT
                     LIAIFFLVLFMNSYFNIVSNVNVNPDGTTLADTYYLSGPDPYYNMRLVDNTVQTGRFP
                     YFSSSDPLLAYPFGKSGGRAPLLVMSAIGFSKLLTPFMSEENAVGYAMQFVPALFGAL
                     LIFPVYFIGKILFGKKAGLIAALLIALIPIHISSGHGSAYALFDHDSFNLLLYFLTFL
                     FLIKSFKEKDTLRRVLYALLAGVPLAALSMVWVEAQFLYTIITIYAVVQLIIDLYTNK
                     VEEGFIMSVAIALFTGFIVSWPVSYAGGVLLDLPFFLAIGILIFGGICILFKRKKIPW
                     VLSLPFVAVIGVGAAVFLYVVFKYPTMFSFFAPLNSIADILYGAGIYGSKVSLTIAEA
                     GTYNMSRTVMSYGPALYWLAWAGFVFLIYQYVKQKGRRDYLMILTLFVINIWLTSTAG
                     RFLNDMVPFIALLSGWIIWFLISKIDYKQMARNIRNAGGGFRGIRKGVRIYHIFGVLF
                     IAFLILIPNGYLALDAAVPSAASKNGTSNMKYDFFGKNHSSAFGSSTYKEQYWVAAYQ
                     WLHKQDANIAEPKKRPGYISWWDYGFYEVAVGGHPTVADNFQDGIPPASNFHTAKSEK
                     EGVAVWIVRLLEGNRKDNNGVAFSDSVIAALQKHLGNNNTTLITSWMLNPTLSPSQGK
                     PIGIEYDKNLSILLSVGEQYPENAYYHDIEQLLNNTLTDEQITWLYHDIQQTTGYSIR
                     YYGVEGYDEQIFNIFAFLGDKSNILTALRTTGKQFFNPEDDFIQVVYTGYTVNTDGSQ
                     GADGQWTAKELNDMPQEQRNRVAVTGTSTVYKADYFNTMFYRAYVGAPAQQDSNGVYQ
                     IPSQQIPCYAMKHFVPAYVSPYPYYGQGRSAVVIAKYYEGAFFNGSISCNNTQLPYVT
                     VAVLDEYGFPHDYMATNQNGSFNLIVPGGNITLIFTYANDVFLKSIKFNSTSDVLYAP
                     VTDAEAMRMNGSKYSRAFSVQVNLSTLEGFVYQDNNNNNSYEPAIDTPLSGITVRLDD
                     YYFGNPVQPVTTDAQGHYIFRNLYPSKYNLSAVEGNYTLLNRKGINIEPNNNTYNISK
                     PKLAGIKGVIYQDSNGDKKYTAGEEESDVHIQLTYTKLNGAQLSVANVTTGASGSYAF
                     PSLIPGEYTLNATKVNASTGYLDFRTSQAITLTVNKTSWVNISLTYAPVNVSGSTRYE
                     DAPISGIPITFAPDKSVANNTATKQSVATSNAQGTYTAALIPGTYNVTVKKTEGSTTV
                     YTFTGKLLLFQGQGTASYNLSLTKVSVTVSGSATYNGSGKANMTIFFTKDLNIKNNTA
                     ITTNVKTNANGMYTIELTPGSYNVTVEQLVNESGQNVTYIGTGGITASSGEAPKVFNI
                     VLTREQST"
     gene            5236..5844
                     /locus_tag="VN377_01725"
     CDS             5236..5844
                     /locus_tag="VN377_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HWR27134.1"
                     /translation="MLNLEEGTKAIQFARQIIQNYVQQTENSIPALGSLFQEKQGVFV
                     TLHTYPDHELRGCIGIPLPVMSLQSAIIEAAQSVTQDPRFPPLQEEELDAVLVEITIL
                     TTPQLIRVKEPKEYPAQIEIGRDGLIVEHGFYKGLLLPQVPVEQAWETEEYLSHTCMK
                     AGLSPDAWFDKNTKISKFQGQIFTEIKPHGPIQETHLDGFHD"
     gene            5825..>6889
                     /gene="pyrC"
                     /locus_tag="VN377_01730"
     CDS             5825..>6889
                     /gene="pyrC"
                     /locus_tag="VN377_01730"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWR27135.1"
                     /translation="MTDFTIEGKIYHNGSFEQACLAVEQGKIVDIKKTLKADTHLNVG
                     TNLILPAGIDLHVHFRDPGFTHKEDFSTGSAAAAFGGISCVFDMPNTQPQTISMQTLR
                     EKTHLASQKNVVDFGLYAAVSDENIGRLAELSTLCAGFKIFLGNSTHSLRLSPQNLRV
                     ALVETNRTKKITLLHAEDEHCLKIHRDREQSLVDHLRCRSAECEEAALRAILQNTHGL
                     SSPIHICHLSSCEGFEVLRKRPTNMSVGVTPHHLFFDVHSLNTKQAFYKVNPPIRSSY
                     DRDTLWYGINNGLIDIFESDHAPHTEEEKNVDFDSAPSGVPGVETLYPLLLAAMKKGQ
                     ITLDTLLALLCEKPAQLLHLP"
BASE COUNT         1960 a         1702 c         1454 g         1773 t
ORIGIN      
        1 ctcttataaa aactttctta cgaaatcact aaaaagacat ggaccattta cggtgtcgct
       61 ttatgtttat acttggggga acctcagcaa aaaacgttgc gacaaatcta gcgaaccgac
      121 ttcaacaacc gcaagtgcaa accacctaca agcgattccc tgacgatgaa ttttacgtcc
      181 gcctccatga caacatagca ggtcaggacg tcctcatcgt tcaaaccgca taccctgacc
      241 aaaaaattgt cgaactcttt cttcttcagg atgctgtcca tgaggcaggt gcgaagacga
      301 ttacagtcat cctcccttat tttggctaca gccggcaaga taaaaaattc gaagacggtg
      361 aagcaatcag cgccagagcc atcgcccagc acatcagcct gcatgctgac tgtgtcatca
      421 ccgttgaccc gcacaaggag catattctga aattcttcac ggttccagca tactgctgct
      481 ccgctgtctc atccatcgct cagtacctca aagaaaaaaa cattgatttc gtccttgccc
      541 ctgataaagg tgcaaaagag cgagccaagg aagcagctgc actcatcaac tgcgagtacg
      601 attacctgga aaagacgcgc atcgatgggt cgaccgtaaa aataaccccg aaaaaacttg
      661 atgctcgtgg gagacacgtc gccatcatcg atgacatcat ctccactggc agcaccatgg
      721 ctcattccat caaggaatta aagaaacagg gtgcggcctc tgtctcggtc gcctgcactc
      781 atgggttatt tgtgggtggc gcaaaagaga aactcctcgc tgcagattgc gatgagatca
      841 tatcaacgga tacgatagaa acggagtata gtaaagtcag cgccgctgaa tgtatcgcag
      901 aaaccctcct gaaaacaatc tgaaacacaa aaaattaagg agattctttt ggagataagt
      961 tatttataag accatcaatt tacggttgta accaaccgag ggtaaaactt atgcggaaac
     1021 gagcacaatt taaaatgcca atgcaatcag aaccagttcc atcgtcgcaa tcaacaccgg
     1081 tcacagccgc cccagaacga aaacgatttc gcgtaaagaa aaactggtgg atgggggtca
     1141 cactgatagc gatattcttc ttggttctct tcatgaactc ctatttcaac atcgtatcaa
     1201 atgtgaatgt gaacccagat ggaacaaccc tcgcagatac ctactatcta tcaggtcctg
     1261 acccatacta taacatgaga ctcgtagata acaccgtaca aactggtcgc tttccgtatt
     1321 tttcatccag tgatcccctc cttgcttatc cattcgggaa atcaggagga cgtgccccat
     1381 tactcgttat gtctgcgatt ggttttagca aattgctcac cccgtttatg agcgaagaaa
     1441 atgcagtagg atatgcgatg caatttgtcc cagcgctttt cggagccttg cttatcttcc
     1501 ctgtctattt catcggaaaa atcttattcg gcaagaaagc aggtcttatc gctgcgctct
     1561 taatcgcact catacctatc catatcagct cagggcatgg atctgcatat gccctgtttg
     1621 accatgactc cttcaacctc cttttatact tcctgacgtt tttattcctt atcaaaagct
     1681 tcaaagaaaa agatacccta cgacgtgttc tgtacgcact ccttgcaggt gtcccactgg
     1741 ccgccctttc catggtctgg gtagaagcgc agtttctcta tacgataatc accatatacg
     1801 cggtcgttca actcatcatc gatctctata caaataaagt tgaagaaggg ttcatcatga
     1861 gcgttgctat tgcgctgttc accgggttta tcgtctcatg gcctgtctcc tatgcgggtg
     1921 gcgtcctctt ggatctcccg ttcttcttag caattggtat cctgatcttt ggtggtatct
     1981 gcatcctgtt taaaagaaaa aagatacctt gggttctttc cctccccttc gtcgctgtta
     2041 ttggtgttgg tgctgctgtt ttcctttatg tcgtatttaa gtatccaacc atgttctcat
     2101 tttttgctcc tttaaattca atcgctgata tattatacgg tgctggaatt tatgggtcga
     2161 aagtatcctt gacgatcgca gaggcaggga catataatat gagtcggacg gtcatgtcct
     2221 atgggcctgc gttatactgg cttgcctggg ccggttttgt tttccttatc tatcaatatg
     2281 tgaaacagaa aggacgacgg gattacctca tgatccttac cttgttcgtc atcaacatct
     2341 ggttgacctc gactgctggt cgtttcctca atgatatggt gccatttatc gctctgcttt
     2401 caggatggat tatttggttc ctcataagca agattgatta caagcaaatg gctcgaaaca
     2461 tacggaatgc tggtggtggt ttccgaggca tacgaaaagg agtgaggatc tatcatatct
     2521 tcggggttct ctttatcgct tttcttatct tgatcccgaa tggatacctc gcacttgatg
     2581 cagcagtacc ctccgcagca tcaaaaaacg gtaccagcaa tatgaaatat gatttcttcg
     2641 gtaagaatca ctcctctgcg tttggttcaa gtacctacaa agaacaatac tgggttgccg
     2701 cctaccagtg gttacacaaa caagatgcaa acatcgccga accaaagaaa cgaccagggt
     2761 acatctcctg gtgggactat gggttttatg aagtcgcggt cgggggacat ccaacggttg
     2821 cggacaattt ccaagacggg attccgccag cgtcaaattt ccataccgca aaaagcgaga
     2881 aagaaggtgt cgcagtctgg attgttcgtt tattagaagg aaatcgaaaa gataacaacg
     2941 gagtggcttt ctctgattcg gtcatcgccg ctctacagaa acatctgggg aataacaata
     3001 cgaccctgat cacttcctgg atgcttaatc ctacactatc tccatcgcag ggcaaaccca
     3061 tcggaataga gtacgacaaa aatctcagca tactgctctc tgttggtgag caatatcctg
     3121 aaaacgcgta ttatcatgac atagaacaac tgttaaacaa tacgttaacg gatgaacaaa
     3181 tcacctggtt gtatcatgac attcagcaaa cgacagggta cagtattcga tattatgggg
     3241 tggaaggtta tgatgagcag attttcaaca tcttcgcgtt ccttggtgat aaaagcaata
     3301 tcttaacagc acttcgaacc actggaaagc aattctttaa tcctgaggac gactttattc
     3361 aggttgtcta taccggttac acagtcaaca ccgatggatc tcagggtgct gatggtcaat
     3421 ggaccgcaaa ggaactcaac gacatgcctc aggagcagag aaaccgtgtc gccgtcactg
     3481 ggacatcaac cgtctataaa gctgattatt tcaataccat gttctaccgt gcctacgttg
     3541 gagcccctgc tcaacaggat tcgaatggag tctatcaaat ccccagtcaa cagatcccct
     3601 gttatgcgat gaagcatttc gtcccagcct atgtttcacc ctacccgtat tatgggcagg
     3661 ggcgaagcgc agtggttatc gcgaaatatt atgaaggggc tttctttaac ggttcgattt
     3721 cctgtaataa cacccagctt ccctatgtga ctgtggctgt attggatgaa tatgggttcc
     3781 cgcatgatta tatggcgacc aatcaaaatg ggagcttcaa tcttatagtt cctggtggga
     3841 acattacatt gattttcacc tacgccaatg atgttttcct gaaaagcatc aagtttaaca
     3901 gtaccagtga tgtgttgtat gcaccggtca ctgatgcgga agccatgcgt atgaatggct
     3961 cgaaatattc acgggctttc agcgttcagg taaacctctc gaccctcgaa ggatttgtgt
     4021 atcaggataa caacaacaat aattcgtatg aacccgctat tgatacacca ctctccggta
     4081 tcaccgttcg attagatgat tattatttcg gcaacccggt tcaaccggtg acgaccgatg
     4141 cccaaggaca ctatatcttc aggaatttat acccgagtaa atacaatctc tctgctgtgg
     4201 aaggtaacta taccctcctg aacagaaaag gcatcaatat cgagcctaac aataacacgt
     4261 ataatatttc aaaaccaaaa ctcgctggaa tcaaaggtgt gatctatcag gacagcaatg
     4321 gtgataagaa atacaccgct ggcgaggaag aaagcgacgt acacatccag ttgacctaca
     4381 ccaaactcaa tggtgctcag ctatcagtcg cgaatgtgac cactggagcc tcaggttcct
     4441 atgcgttccc gtcattaatc cctggagaat acacgttgaa tgcgacaaaa gtcaatgcaa
     4501 gcaccgggta tcttgatttc cgcacctcgc aagcaatcac acttaccgtg aacaagacct
     4561 cctgggtgaa catctcactt acctacgcac ctgtcaatgt cagtggatct accaggtatg
     4621 aggacgcacc aatctctgga atcccgatca ctttcgcgcc ggacaaatcc gttgccaaca
     4681 atactgcgac caaacaaagc gtagcgacat cgaatgcaca agggacttat accgcagcac
     4741 tcatcccagg aacctataat gtcacggtca aaaaaacaga aggaagtacc actgtgtata
     4801 cgtttacagg aaaactcctt ctcttccaag gtcaagggac tgcatcgtat aatctctctt
     4861 tgacaaaagt atcggtaacc gtcagtggta gtgcaaccta caatggctca ggaaaagcaa
     4921 acatgaccat tttcttcacc aaagacctca acattaagaa taacactgcg atcaccacta
     4981 atgtgaagac caacgccaat ggaatgtaca ccatcgaact tacgccaggt tcctataacg
     5041 tgaccgtcga acaacttgtc aatgaaagcg ggcagaacgt cacctacatc ggcaccggag
     5101 gaatcacagc gagcagcgga gaagcaccaa aagtgtttaa catcgttctg acacgagaac
     5161 aatcaaccta aacaaggaga ggagcgttcc ctctccacca cattttattt aatacttctc
     5221 tgtatactat ccccgatgtt gaatctagag gaagggacaa aggcgattca gttcgcacga
     5281 cagatcatcc agaactatgt ccagcagacg gagaattcca tacctgctct tgggtcgttg
     5341 tttcaggaaa aacaaggggt ttttgtcaca ctccatacgt acccggacca cgagttacga
     5401 ggttgcattg gcattcctct ccctgtgatg tcgttacaat ctgcgataat cgaagctgct
     5461 caatccgtca cccaagaccc aagatttcca cctttgcagg aagaagaact tgatgcggtt
     5521 ctcgtagaaa tcaccatact gacgacacca caactcatta gggtaaaaga accgaaagaa
     5581 tatcctgcac agatcgagat tgggagggac ggattaatcg tggagcacgg tttttataaa
     5641 ggtttattgc tccctcaggt ccctgtcgaa caagcatggg aaacggaaga gtacctttcg
     5701 catacatgta tgaaagcagg attatcccct gatgcctggt tcgataaaaa cacaaagatt
     5761 tcaaagtttc agggacagat ctttactgaa ataaaacctc atggacctat ccaggagacg
     5821 caccttgacg gatttcacga ttgaagggaa aatctaccac aacggatcat tcgaacaagc
     5881 ctgtcttgct gttgaacaag gaaaaatcgt tgatataaaa aaaaccctga aagcagacac
     5941 tcatctcaac gttggtacca acctcatcct tcctgctggt attgatcttc atgtgcattt
     6001 ccgcgaccct ggttttaccc ataaagaaga tttttcgacc gggtccgcag cagctgcctt
     6061 tggcgggata tcctgtgtct ttgatatgcc taatactcag cctcaaacca tcagcatgca
     6121 gaccctgagg gagaagacgc atcttgcgtc acaaaaaaac gttgtcgatt tcgggctcta
     6181 tgccgctgta agcgatgaga atatcggaag gcttgcagaa ctctccacgt tatgtgctgg
     6241 ttttaaaata tttttaggaa acagcaccca ttcccttcgg ctcagcccac aaaatctgag
     6301 ggtcgcatta gtagaaacca atcgaacaaa gaagattacc ctcctccatg cagaagacga
     6361 gcattgtctc aaaatacata gggatcgaga acagagtctt gttgatcatc tccggtgtcg
     6421 atccgcggaa tgtgaagaag ccgcactcag ggctatcctc cagaacactc atggtctttc
     6481 ctcccctatt catatctgtc atctctcgtc ctgtgaaggc ttcgaggtgc ttcggaaacg
     6541 accgaccaac atgagtgtcg gagtcactcc tcatcatctg ttcttcgatg ttcatagtct
     6601 gaatacgaaa caagcgttct ataaggtgaa tcccccgatc cgatcgagtt atgatcgtga
     6661 tactctctgg tatggtatta ataatggact catcgatatc ttcgaatcag atcacgcgcc
     6721 tcatacagag gaagaaaaaa acgttgattt cgacagtgcc ccttcagggg tacccggggt
     6781 tgaaaccctc tatcccctgc tcctggctgc gatgaaaaaa ggacagatca ctcttgatac
     6841 tctcctagcc ctcctctgtg aaaaacctgc acagctcctt catctcccg
//
LOCUS       DATMXJ010000059        10440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_27069, whole genome shotgun sequence.
ACCESSION   DATMXJ010000059 DATMXJ010000000
VERSION     DATMXJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27069"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..534
                     /locus_tag="VN377_01735"
     CDS             139..534
                     /locus_tag="VN377_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR27136.1"
                     /translation="MKKARSPIDKNIETAVHELLDSRAESRIYVYLLRKNRARSEEII
                     RGTKLHPSTVRELLSKMYDNKVIFREKIKTDSIGKNPYLYSAVSPILLLQRRVKIIES
                     RLNKIANLTGKSNGNKYVQITIDDQRGEL"
     gene            531..2765
                     /locus_tag="VN377_01740"
     CDS             531..2765
                     /locus_tag="VN377_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWR27137.1"
                     /translation="MKIAHSLATLLVKRPKTVLIVYTVITIIIGIQIRNVYMQSDLTA
                     FLPQNDPTLQLWNKINQEFQIGSTIVVYINADDIRDPYVLREMDRVSTKINTYELDKG
                     KTDGIFSVTSIAQLIKEENAKPELPYNLGGTGKYEIPDDPTLINTYMARIQSMEGTLF
                     LNTYQDTVMIFQLADNANYDQILQDLKGAIQKDARYSEMTITGGLAAQRAMRAQTFQS
                     LTIVFPLAALFVAIDLFFFHRTLKGFIIGFLPLSYSLVLTFGVLGIVQPELTILTIAV
                     AALLLGLGIDYSIYYANRFAEECTLSDKIERVECTLGRTGKAVLMCALTTIIGFGSLM
                     TSNMPPMVTFGFGCAIGISFAFISATILVPCLCLILKFEKHEVHHGWKRFANIVVDNR
                     KRLFAVSCFFAILSLIVLPQVKTDVNFLEMAPGGIPEVQKLLEYSQKFGKGANFNALL
                     VETDAQGLTDPEVIKAIYNMEDQIRATGASAYSIADEIKKVNDIFERNLLLQQIAKFV
                     GVDSIILDKIAKKGLVDEEYSKTIIVVSFSTGTSTAELETLVNQINDIAQSASIPQNG
                     VVSKLAGQDVVSVEVNKQIMGSQASALIVALLLVLACLIIGFNSLLIGGLALLPVLFV
                     LMWEPGSLVMLDIPLSVVNITVASIMIGTGIDYSIQTTQRMKEEIAKGVSKIEAMKTT
                     IETSGWSIVGAATTTMVALLATFAVNIPILHQFSILVMVLILFSLLASLTILPTICTS
                     RFMK"
     gene            2906..3451
                     /locus_tag="VN377_01745"
     CDS             2906..3451
                     /locus_tag="VN377_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWR27138.1"
                     /translation="MMLESMIVPVPSEFVMPFAGFLVAQGSFNAVLVILVSTLGSITG
                     SLIFYYIGKTQGHNLVQRYGKYVLVNSEDIKKTEAWFNKRGDLTIFLARLIPVVRHLI
                     SLIAGIGKMNVKKFTMYTILGAALWNGILTYLGFLLGQHWNEVSQYMENLDLVIVVLL
                     IVGCLYFIYRHLRRAKKTTEQ"
     gene            complement(3458..3739)
                     /locus_tag="VN377_01750"
     CDS             complement(3458..3739)
                     /locus_tag="VN377_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27139.1"
                     /translation="MKTNVKKMKNTDNQRMITIQRLLLRLELWFAPLLLCIPLIVAMV
                     FLLEWYLKGFTTGSSEYDGELLLGLLLLVGNLVFDIPFVRSLRTLKRKQ"
     gene            complement(3736..4446)
                     /locus_tag="VN377_01755"
     CDS             complement(3736..4446)
                     /locus_tag="VN377_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichol kinase"
                     /protein_id="HWR27140.1"
                     /translation="MLFSENTKDALKTTKQRMSKYQDEFLEQLTAIEVDAHWFRRVFH
                     TFAASFLFYYLLPDEEWINVIKIIVPVILVFCLIVIEYHRLRGALDHQRFFGLRNYEK
                     KRPASYLYFGIAVLLLFLLFPQQIAIPCILCASFTDPVIGETRYHLGKKKAYIIGFII
                     SLFFFLITWFRADWRILILVSVIGAAGALAGEAKKLRYIDDDFMIQMLPALLLLLFWQ
                     GSLAFGINILPPKIILPI"
     gene            complement(4482..5159)
                     /gene="rpiA"
                     /locus_tag="VN377_01760"
     CDS             complement(4482..5159)
                     /gene="rpiA"
                     /locus_tag="VN377_01760"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HWR27141.1"
                     /translation="MKKQAAEKAVQYIEEGMIVGLGTGSTVEFAVKKIGEMVKNGLKI
                     EAIPTSLKTKRLATELKIPLIDLDDRVEIDLTIDGADEVDSNLNLIKGGGGALTREKI
                     IAYHSKKVIIIVDETKIVKGLGCDCFLPVEVTKFGWSATKKVLEGLGCTAELRKIMDE
                     AFITDNQNYIIDCDFGKITDPETMEKEINNIPGVLENGLFIGLADEVIVGSKQGMMTL
                     ERQDIVQ"
     gene            5327..6040
                     /locus_tag="VN377_01765"
     CDS             5327..6040
                     /locus_tag="VN377_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HWR27142.1"
                     /translation="MSDRELDSLGRIMAGVLRHFPEKLGVTMDGHGWVDISEFVEAVG
                     GSRSGFQWLRDHHVEAIALTDPKGRYQVDGGMIRATYGHTIDIRLDDLPLAEIDDFYY
                     PVTEEEIDIILEGGLNPIDRKHVHLSGSMEKAIEAGKVRTEIPLILHIDGKKAIEDGV
                     KIYRAGKDVYITERIDAKYISKAEVSDVSTSIEDSTTETETEEAPPEPTHSEETEEKP
                     SPKPKRKTTKKTKKTKAKE"
     gene            complement(6047..6667)
                     /locus_tag="VN377_01770"
     CDS             complement(6047..6667)
                     /locus_tag="VN377_01770"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HWR27143.1"
                     /translation="MRIAKGCWSWVAPPLVLSVLFLLLSWFSLWFLAGFIPVFCVLVF
                     FLIFFRDPERTIAPGIIAPADGQIRDIKQEKNQCLISTFMEVNNVHVNRMPLDGRITK
                     MTHFPGYHLRAWKKESDLNERVVITVDSAIGEVTVVQLAGLIARRVYPYIKEGDVLKK
                     GDRIGIIRLGSRVDVYLPADMIQSIPVKIGDPVKAGEDTIAFLKEK"
     gene            complement(6664..8676)
                     /locus_tag="VN377_01775"
     CDS             complement(6664..8676)
                     /locus_tag="VN377_01775"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR03844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HWR27144.1"
                     /translation="MKYTLKCLKCGKTYSGDHYICSCENKCDALLKTVYEQKSLTIDE
                     SYPGIWQYHQWLPIQKIDQRLIDHGSCFRTYRSTKLAAHLGLQNLIICLNAYPHCHTG
                     SFKDLEAEVTFQRVMSTFDAGKPLVMSSDGNNAISFVHYSNVIRYPIFLFATEDARIK
                     RIWSFSKSNLYTRLFPIYGDYYDAINLANQFSRVEGYLSEGGYRNVGRRDSVGTMMLD
                     ATRFLGRIPDHYFQALGSGPGAIAAFEAAERLILDGRFGKTLPKIHGSQNHPYAPMYE
                     AWMNHSRTIDERFQKESAKQMIDQTMAHVLTNRFPAYSLMGGIYDVLTETKGEMYSVT
                     NEEIEKAQQLFEKLEGISIVPEAGVTIASLQQALEKQTISPQESILVNITGGGRKEIK
                     KERMHIEPYRFLTKKLQISQRTLEEKIQHSKDLIQQALQTYGDKVAVACSWGKDSMVL
                     LHLARSINPDVPIFSVLTIHKPKDTFDFIVKVVHQYHLTPRIYMVGETIPEVFMENNI
                     EVTLLPMEQYRIQEEKIKNETGHEIYYADPDLCCKLLKVVPVRYAYTTLGLQAWFSGL
                     RSTEGFTRKYIAEIEKISDHETKINPLLSWTEEEIWKYIKNNKIPAHPWYKKKFSDGR
                     KIRSLGCEPCTVPIFDHESERDGRWRKTLKKGGECGIHTRSASERI"
     gene            8742..10145
                     /locus_tag="VN377_01780"
     CDS             8742..10145
                     /locus_tag="VN377_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27145.1"
                     /translation="MNPFLNPVSVFPLLKNYLLDTRRLNTNSSEKIRKYRNKVFLRTL
                     QYADTVPLYHKKYAEAGLLLQDIHGIEDATKIPFVSKPDLVAHYPNGLLPLDYNRKNA
                     HLVSTSGSTGKQVSLYTDLSVFIGGIGALVRTYDQLHLNWRTSRIANIGNFNPNMADS
                     TAENLFYSKGSFVYGKNRRFSINAFLPMKEIVAQLDRFKPDIIYSYPATFFQIAYFKR
                     KGLGEHINPKAFLTGGSVLEPYTRAYVEETFGCKMHNVYESVESLGAPMAFECNEGTW
                     HINYDFFHIEAIDEEMKPVEKGKRGHIVVTRLYGKATPIVRYTGMDDWVAISEDDGCP
                     CGLHTPVLKGGVEGRKSDSVVLPDGRVFPAASFASLYSILNDLKTRKVKQFQIVQKKI
                     DEIDILVIIDDDLRNVGPSLDVIFEKIKEIHQKKAGPGVQITVKEVQDIPSQKGKPAP
                     LVISHIRPEKGYKVIDE"
     gene            10234..10404
                     /locus_tag="VN377_01785"
     CDS             10234..10404
                     /locus_tag="VN377_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27146.1"
                     /translation="MRTRIFQLRSWTKKIKIEEKTTMDVHKVMSTTEVIITSIAGPAG
                     VIIYFLLKKKKI"
BASE COUNT         2858 a         2311 c         2334 g         2937 t
ORIGIN      
        1 ctttgcattc ttagtatttt tatcgttttt taggcgataa aatgttttgt cggctgagaa
       61 acgacaaaat ggaaaggtat ttaaactcag aaaccaacgt tttttattaa aagtatcaca
      121 taaatatggt gcgcagtcat gaaaaaagcc cgttctccta tcgataaaaa cattgagaca
      181 gcagtccatg agttactgga ttccagggca gagtctcgta tatatgtcta cttacttcgg
      241 aagaatcggg ctcggagtga ggagatcatc cgaggcacca aactccatcc tagcaccgtc
      301 cgagagctcc tttctaagat gtatgataat aaggtcattt tcagggaaaa aatcaaaacg
      361 gacagcattg ggaaaaatcc ctacctttat agtgcggttt cacctattct gttactgcag
      421 cgtcgagtca aaatcatcga atcacggctt aacaaaatcg ctaacttgac cgggaaatcc
      481 aacgggaaca agtacgtaca aatcacgatc gatgaccaga gaggtgaact atgaaaatag
      541 cgcactcttt agccacactt ttggtgaaac gaccgaaaac agttctcatt gtctacacgg
      601 ttatcactat catcatcgga attcagatcc gtaatgtata tatgcagtct gacctcactg
      661 ctttcctccc gcaaaacgac ccaacgctcc aactctggaa caaaattaat caggagttcc
      721 agataggatc tactattgtc gtgtatatca acgccgatga tatccgtgac ccgtatgtcc
      781 ttcgagagat ggatcgggtg agtaccaaga ttaacacgta cgaacttgat aaaggaaaga
      841 cggacggtat cttctcagtc acgagtatag ctcagctgat aaaggaggaa aatgcaaaac
      901 ctgaattacc ttataattta ggtggtaccg gaaaatatga gattcctgat gatccaacat
      961 taatcaacac ctacatggct cgtatccaat caatggaagg caccctcttc ctcaatacat
     1021 atcaggatac tgtcatgatt ttccagttag cagataatgc aaattatgac cagattctcc
     1081 aagacctaaa aggagcaata caaaaggacg ctcgttattc cgagatgaca ataacagggg
     1141 gtcttgctgc acaaagagca atgagagccc agacgttcca atctctcacg attgtctttc
     1201 cgcttgcagc gttatttgtc gccattgacc tgttcttttt ccataggacc ctgaaaggtt
     1261 ttatcattgg tttcctccct ctcagctact cattagtttt aacatttgga gttcttggca
     1321 ttgttcagcc tgagttaaca attttaacca tagctgttgc ggctcttctc ctaggtcttg
     1381 gtattgatta ttcgatttat tacgcaaatc gtttcgcaga agagtgtacc ctgagtgata
     1441 aaattgaacg agtagaatgc accttgggac gaactggaaa ggcagtgtta atgtgcgctc
     1501 tcactacgat cattggtttt ggttcgctga tgacgtcgaa catgccgccg atggtgacct
     1561 ttggtttcgg ttgcgctatt gggatcagct ttgcgtttat ttctgcgacg attcttgtcc
     1621 cttgtctgtg tctcattttg aaatttgaga aacatgaggt gcatcatggg tggaaacggt
     1681 tcgctaatat tgtcgtggac aacagaaagc gattgtttgc ggtgtcttgt ttttttgcaa
     1741 tcctctctct catcgtcctt cctcaggtga aaaccgatgt gaactttctc gaaatggctc
     1801 ctgggggaat tccggaagta caaaaactcc ttgagtattc gcagaagttt gggaaaggag
     1861 caaatttcaa tgcacttctt gtcgaaaccg atgctcaggg acttaccgac cctgaagtta
     1921 ttaaagcgat ttataacatg gaggatcaaa tacgagcgac tggtgcttct gcgtactcga
     1981 ttgcagatga aatcaaaaaa gttaacgata ttttcgaacg aaatttactc cttcaacaaa
     2041 ttgcaaaatt cgtaggtgtc gatagtatta tcctcgataa gatagcaaag aaagggttgg
     2101 tggatgaaga atattcaaag accattatcg tcgtgtcctt ttcaacgggg acaagcactg
     2161 cagaattaga gacgctcgtg aatcagataa atgatatcgc tcagagtgcg agcatcccgc
     2221 aaaacggtgt tgtctcgaaa ctcgccggcc aagatgtcgt cagtgttgaa gtgaataaac
     2281 aaatcatggg ctcccaagca tcagctctta tcgtagctct tttattggtc ctggcttgtc
     2341 tcatcatagg attcaattct cttctgatcg gagggttggc tttgcttcct gtcctctttg
     2401 tccttatgtg ggaaccagga tcccttgtca tgcttgacat tccactttcc gtcgtcaata
     2461 ttactgttgc gtcaatcatg attggtacgg gtatagatta cagtattcaa acaacacaac
     2521 ggatgaaaga agaaatcgcc aaaggcgttt caaaaattga agcgatgaaa acaacgattg
     2581 aaacctcagg gtggtctatc gttggagcag ccacaacaac catggttgcg ctccttgcaa
     2641 catttgcagt gaacattcca atcctacatc aattcagtat tcttgttatg gtcttaattc
     2701 tgttttcctt actcgcaagt cttactattt taccgactat ttgtacatcg agatttatga
     2761 aataacgaga cagaatgaag tattaataac cctgcttgat gttccttggc gaccaatgtc
     2821 atttctcaca ggtatttttg cttttatcgg gagttttgcc ctttcggcaa tttctcaact
     2881 tggttatgcc ggtatatttt ttctcatgat gcttgaaagc atgattgtcc ctgtgccctc
     2941 agaatttgtc atgccatttg cagggttcct tgtcgcacaa ggttctttta atgcagttct
     3001 cgtaattctc gtcagcaccc ttgggagcat tactggctca cttatttttt attacatagg
     3061 aaaaacacag gggcataatc ttgttcaaag atatgggaag tatgttctcg ttaattcaga
     3121 agatattaaa aaaacagaag catggttcaa caaacgaggg gatcttacca tatttctcgc
     3181 tcggcttatc ccggtcgttc gccatctcat ttcgctgatt gctggcatcg gaaagatgaa
     3241 tgtgaaaaaa ttcacaatgt acactatcct tggtgcagca ttgtggaacg gtatccttac
     3301 gtaccttggt ttcctccttg gtcagcactg gaatgaggtc agtcagtata tggaaaacct
     3361 tgaccttgtc attgttgttc tcctcatcgt aggatgtctg tacttcatct atcgtcacct
     3421 ccgaagagcg aaaaaaacca cggaacaata aaaccggtca ttgttttctt ttgagcgttc
     3481 gtaaggaacg tacaaacggg atgtcgaaca cgagatttcc gactaacaga agcagaccaa
     3541 ggagtaactc cccgtcgtac tcagaagatc cggtggtaaa tccttttagg taccactcca
     3601 ataagaacac catagcaacg atgaggggga tacacagaag caacggggca aaccataact
     3661 ctaaccgtaa taataacctc tggatagtta tcattcgttg attgtctgtg tttttcattt
     3721 ttttcacatt cgtcttcata tcggtagaat aatttttggt ggtaggatat tgatgccgaa
     3781 agccagagat ccttgccaga agagcaagag gagaagggcg gggagcatct gaatcatgaa
     3841 atcatcatcg atgtatctga gtttctttgc ctcaccagcc aaggctcccg cggcgccaat
     3901 cacagagaca agtatcagta tccgccagtc tgctcggaac catgttatga ggaaaaagaa
     3961 cagggatatg ataaacccga ttatgtacgc cttttttttc ccaagatggt atcgcgtttc
     4021 accaatgacc gggtccgtga aggaggcaca caggatgcag gggatcgcta tttgctgagg
     4081 gaacaggaga aataacagga ggacggcgat accaaaatac aggtagcttg ctggtcgttt
     4141 cttctcataa tttcgcagac cgaagaagcg ttggtgatca agggctcctc gcagtcggtg
     4201 atactcgatc actatcaggc agaagacgag tatcaccgga acaattatct tgattacgtt
     4261 tatccattct tcatctggca ggagataata gaataagaat gatgcagcaa acgtatggaa
     4321 cacacggcgg aaccaatggg cgtcaacctc gattgctgtg agctgctcta aaaactcatc
     4381 ttggtatttc gacatccttt gttttgttgt ctttaaggcg tcttttgtgt tctctgagaa
     4441 aagcattcgt ttaagccatc cttctaagga tactacaagg gttattgtac gatatcttgc
     4501 cgctccagtg tcatcatccc ttgtttactc cccacgataa cctcatcagc tagaccgata
     4561 aataacccat tctccaggac accagggatg ttgttaatct ctttctccat tgtttcagga
     4621 tccgtgatct tcccaaaatc acaatcgatg atgtaattct ggttatctgt gatgaacgct
     4681 tcatccatga ttttgcgcaa ctctgctgta caccctaatc cttctaatac cttcttggtg
     4741 gctgaccagc cgaactttgt gacctcaacg gggaggaaac agtcgcatcc aagacctttt
     4801 actatctttg tttcatcaac gatgataatg accttcttcg aatggtacgc gatgatcttc
     4861 tctctggtga gagcacctcc accaccttta attaaattca gattcgaatc gacctcatca
     4921 gcaccatcga tcgtaagatc aatctcgacc cgatcatcaa gatctattaa cgggattttt
     4981 agctctgtgg ctaacctctt ggttttcaac gaggtgggga ttgcttcgat tttcagaccg
     5041 ttttttacca tctccccaat tttttttact gcgaattcaa cagtggagcc tgtacccagt
     5101 ccgacaatca tcccctcttc aatgtactga actgctttct ccgcggcttg ttttttcatt
     5161 tcttcttgtt ccactgggtc accaacaggg taatggaaca aggaatacaa aaacttatac
     5221 atcttatgct attacggaac gtgaaactat gctaggaaaa tgtgacgaac acgggtatta
     5281 ccgaggggaa atatgtccga tatgtaagac aaaaggtaag tttctcatga gtgatcgtga
     5341 gcttgactcc cttggtcgta tcatggccgg agtcctccgc catttcccag aaaaacttgg
     5401 agtgaccatg gatggtcacg ggtgggttga tatctctgag tttgtcgaag cggtcggtgg
     5461 gagcaggtct gggttccaat ggctccgtga tcatcatgtc gaagcgatcg cactgaccga
     5521 tccaaagggc agatatcagg tagatggtgg gatgatacgg gcgacctacg ggcataccat
     5581 cgacatccga cttgatgatc taccactcgc ggagattgat gatttttatt acccggtcac
     5641 tgaagaggaa atcgacatca tcctcgaagg gggcttgaac cctatcgatc gtaaacacgt
     5701 ccatctgagc ggaagtatgg aaaaagcaat tgaggcgggg aaggttcgaa ccgagattcc
     5761 cctcatttta cacatcgatg ggaagaaagc tatagaagat ggcgtgaaga tctatcgcgc
     5821 agggaaggat gtttatatca cggaacgtat cgatgcaaaa tatatttcaa aggccgaagt
     5881 ctctgatgtt tcaacatcca ttgaggactc aacgaccgag acagaaactg aagaagcacc
     5941 accagaacca actcactctg aagaaacaga ggaaaaacct agtccgaaac cgaaacggaa
     6001 aactacgaaa aagacaaaaa aaaccaaggc aaaagagtaa gagtcttcat ttctctttca
     6061 aaaaagcaat ggtatcttcc cctgctttta ccggatctcc gattttcaca gggattgact
     6121 gtatcatatc cgcagggagg tatacatcaa cccgtgaacc aagcctgatg atcccgatac
     6181 gatccccttt cttaaggacg tccccttctt tgatgtacgg gtagacgcgt cgagcgataa
     6241 gccctgcgag ttgtacgacc gtgacttcac cgatcgctga atcgacggta atcaccaccc
     6301 gttcgttcag atccgattct tttttccaag ccctcaggtg gtaccctggg aaatgcgtca
     6361 tcttcgtaat gcggccatcg agaggcatcc tgttgacatg gacgttgttg acctccatga
     6421 acgtcgagat gagacactgg tttttctctt gcttaatatc tctgatttgc ccatctgctg
     6481 gtgcgatgat ccccggggcg atcgtgcgct cggggtcacg gaagaaaata aggaaaaaaa
     6541 caaggacaca aaaaacaggg ataaaaccgg caaggaacca aagagaaaac caactgagta
     6601 gtaaaaacaa aacactaaga actaatgggg gagcaaccca ggaccaacaa ccttttgcga
     6661 ttctcatatc ctctcacttg ctgaccgtgt atggataccg cattccccgc cttttttcag
     6721 ggtttttctc catcgaccat ctcgttcgct ttcatggtcg aagataggca cagtgcatgg
     6781 ttcacatcct aaagaccgga tttttcgccc atcggaaaat ttctttttgt accagggatg
     6841 tgcaggtatc ttattgtttt ttatgtactt ccatatctcc tcttcggtcc aggagagaag
     6901 cggattgatt tttgtctcat ggtcacttat tttttcaatt tcagcgatat acttcctggt
     6961 aaatccctcg gtggatcgta atccgctgaa ccaagcttgc agaccaaggg tcgtatatgc
     7021 ataacggacc gggacgactt ttaataattt acagcagaga tccgggtccg cgtaatagat
     7081 ttcatgacct gtctcatttt ttattttttc ttcttgtatc cgatactgct ccatgggtaa
     7141 taaggtgact tcgatgttgt tttccatgaa tacctctggt atcgtttcac ccaccatgta
     7201 aatccgtggt gtaaggtgat attggtgtac gacttttacg atgaaatcga aggtgtcctt
     7261 tggtttatgg atggtcaaca cggagaaaat agggacatcg gggttgatgg atcgtgcaag
     7321 atggagaagg accatgctgt cttttcccca gctgcaggcg acagcgacct tgtctccgta
     7381 tgtttgaaga gcttgctgga tgaggtcttt agaatgttgt attttctcct caagtgtcct
     7441 ttggctgatt tgaagttttt ttgtcaggaa ccgatacggt tctatgtgca tccgttcttt
     7501 tttgatttcc tttcttccac caccagtgat gttcacaagg atggactcct gcggtgagat
     7561 cgtttgtttt tctaatgcct gttgtaagga tgcgatcgtc acccctgcct cagggacgat
     7621 gctgatgccc tcgagtttct cgaataattg ttgggctttt tcgatttctt cattggtgac
     7681 gctgtacatc tcccccttgg tttctgttaa cacatcatag atgccaccca ttaaggaata
     7741 tgctgggaag cggtttgtga gcacatgggc catggtctgg tcaatcattt gttttgctga
     7801 ctctttttgg aaccgttcat caatcgttcg agagtgattc atccatgctt cgtacatcgg
     7861 ggcgtatggg tggttttgag agccatggat ttttggtaaa gttttcccaa atcgtccatc
     7921 aaggatcagt cgttctgctg cttcgaatgc tgcgatcgca ccaggccctg agccgagcgc
     7981 ctggaaataa tgatccggga ttcggccaag gaatcgtgtt gcatccagca tcatggttcc
     8041 aacgctgtcg cgacggccaa catttcgata accgccctcg gaaagatacc cttcgactct
     8101 ggagaattgg ttggctaggt tgatcgcgtc atagtaatca ccgtagatgg gaaagagacg
     8161 tgtgtatagg ttggatttgg aaaaagacca gatacgtttg atgcgggcat cttccgtcgc
     8221 aaacaagaaa atagggtacc gtatcacatt ggaatagtgg acgaaggaga tagcgttgtt
     8281 cccatcggag gacatcacga gtggttttcc tgcgtcaaac gtactcatga cacgttggaa
     8341 ggtgacctct gcttcaaggt ccttgaagga tcccgtatgg cagtgtgggt atgcgttcag
     8401 gcagatgatg aggttctgca gtccaagatg cgccgcgagt tttgtgcttc tataggttcg
     8461 aaaacaactc ccatggtcga tgaggcgttg gtctatcttt tgtatcggaa gccactggtg
     8521 gtactgccat atcccagggt aggattcatc gatcgtgaga cttttttgtt cgtagactgt
     8581 ttttaagagc gcgtcacatt tattctcgca agaacagata taatgatctc ctgagtaggt
     8641 cttaccgcat ttcagacatt tcagtgtgta tttcacgcgt cacgccgctt caggcaataa
     8701 taaaatatat taattaatat ttgtatgctc gcgagaagac catgaacccg tttctcaacc
     8761 ctgtgtcagt tttccctctt ctaaaaaatt atctccttga tacgcgcaga ttgaacacca
     8821 attcctcgga aaaaatcagg aaatatcgaa acaaggtgtt cctccggaca cttcagtatg
     8881 ctgataccgt ccccctctat cataaaaaat acgctgaagc cggccttctg ctgcaggata
     8941 ttcacgggat agaagacgct acaaaaatcc cttttgtctc aaaaccagat ctggtcgctc
     9001 attacccaaa cggacttctc cccctggact ataatagaaa gaacgcgcat ctcgtgtcaa
     9061 catcgggttc cacaggcaag caggtttccc tttacacgga tctttccgtt ttcattggtg
     9121 ggatcggtgc gctcgtacga acctatgatc agctccatct gaactggcgg acatcccgga
     9181 tagcaaatat cggtaatttc aaccccaata tggctgattc gaccgctgaa aacctttttt
     9241 actccaaagg ttcctttgtg tatgggaaaa acagacggtt tagcatcaat gcgttcctcc
     9301 ctatgaaaga gattgtggcc cagttagatc gattcaaacc agatatcata tattcctacc
     9361 ctgccacatt ttttcaaatc gcctatttta aacgaaaagg gcttggggag cacatcaacc
     9421 caaaagcctt ccttacgggc gggtcggtgc ttgagccgta cacgagagcc tatgttgagg
     9481 aaacctttgg atgtaaaatg cataacgtgt acgaatccgt agaatccctc ggtgctccga
     9541 tggcgtttga atgcaatgag gggacatggc atatcaacta tgattttttc catatcgaag
     9601 caatcgacga agagatgaaa cccgtagaaa agggaaaacg aggccatatc gtggtcacac
     9661 gactctacgg caaagcgacg cctatcgtgc ggtacaccgg gatggatgat tgggtcgcta
     9721 tctctgaaga tgacggatgc ccctgtggtt tgcatacacc tgtgctgaaa ggcggtgtgg
     9781 aaggaagaaa aagtgatagt gtggtattac cagatggacg ggtgtttcct gcagcttcct
     9841 tcgcaagtct gtacagcatc cttaatgacc taaaaacaag gaaagtgaaa caatttcaga
     9901 ttgttcagaa gaaaatcgat gaaatcgaca tccttgtgat catcgatgat gacctcagaa
     9961 atgtcggccc ctcccttgat gtgatttttg aaaaaatcaa agagatccat cagaaaaaag
    10021 caggcccagg tgtacagatc actgtaaaag aggttcaaga cattccctcc caaaagggaa
    10081 aaccagcgcc cctcgtcatt tcccatattc gacctgagaa gggatacaag gtgattgacg
    10141 agtaatgcac ctgaaatcgt atgggaagtt attctgtttc agaaaatatt accgtctcga
    10201 atgttggtat tgtgtcatcg ttaagcctaa ataatgagga ctaggatctt ccagctacgg
    10261 agttggacca agaaaataaa aatcgaggaa aaaacaacca tggacgtcca caaagtcatg
    10321 agcacgaccg aagtgatcat cacatcaatc gcaggaccag ctggcgtgat catttatttt
    10381 ttattgaaga aaaagaagat ttaaatagag acgtatgtta ttcaattttg agttaattgt
//
LOCUS       DATMXJ010000060         5368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53481, whole genome shotgun sequence.
ACCESSION   DATMXJ010000060 DATMXJ010000000
VERSION     DATMXJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53481"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VN377_01790"
     CDS             <1..641
                     /locus_tag="VN377_01790"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HWR27147.1"
                     /translation="RYTQGALIAKMEQLSLGTKSTRHEIISKLYYRKYVIGGTPTPTS
                     TAIAVVDALINCDVVKPKMTAELEADMNDIAEGKKTLEETVRESRLMLTKVMEELEPE
                     KEKIKENINNAVKAQNTIGPCPKCGKSLMVRVSKKGKRFVGCTGYPECTNTYSLPQQG
                     GLTMTTKLCDACDTPIVQVKMKGRPAWELCLNSDCPKKKKKEIEKPPAAPTA"
     gene            complement(646..3177)
                     /gene="gyrA"
                     /locus_tag="VN377_01795"
     CDS             complement(646..3177)
                     /gene="gyrA"
                     /locus_tag="VN377_01795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWR27148.1"
                     /translation="MANGFINHNTETKMAKITKLMLQDIEKETVEWRDNFDGTLKEPE
                     VLPAVLPNLLINGSSGIAVGMATNMAPHNICEVIDGTIRMIDAPETSTAELMEIIKGP
                     DFPTGGTICGRGGILSAYSTGRGSLVVRAKTSIEEGEQKNKIIVHELPYQVNKSVLLQ
                     TIAELVKEKKLEGISDLRDESDRKGMRIVIELKRDAIDDVVLNQLYEHTELQSSFGVL
                     NLAIVKGEPKVLMLREILQYYIEYRIEIITRRTAYDLKKAREKMHILEGLMIALRNID
                     EVIKIIRQSKEVEEAKSRLMKRFNLSEIQAKAILDMRLQKLTGMEIESVEKEYNETKQ
                     LIEELEALLADKQMILAEIKRELLEIKEKFGDARRTQIVEGEAGIDIEDLIPVQEVVV
                     TITKDGYIKRIPTETYRTQHRGGKGLIGVRPKEEDFVVDSFITSTHDYLMFFTNHGRV
                     YWMKGYKIPEGDRHAKGKAIINLLPRLEEGEKIETAVPIHEFDDTHFLIFTTKQGTIK
                     KTVLSAYGNIRVNGIIAIKLEEGDELMGVELSDGTKTVMIATAGGQACRFNERELRPM
                     GRATHGVIGIRLKNKKDRVVAMTVVDITGSLFTITENGFGKRSPIDEYRMTHRGSKGV
                     RTIVTNERNGSVVFVSQVNDENELIITTEHGMTVRIPVRDIREQGRNTMGVRIMRLNE
                     GDKVVSVTITKILEENGKEAAQVEAMERMQIGMVEEKTEPIEAPKPSVEQKEPAQEVK
                     KQEPTLEPKQPVKKEAMPKKPMKIAKPKKEKPASVKKIKTSKPKKVTLKPVKKPKKTK
                     GRSAKKTKTVKSKKAQSKPVRKPKKIKPAKKVKAKKPQKKKPKRT"
     gene            complement(3163..4824)
                     /locus_tag="VN377_01800"
                     /pseudo
     CDS             complement(3163..4824)
                     /locus_tag="VN377_01800"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            complement(4836..>5368)
                     /locus_tag="VN377_01805"
     CDS             complement(4836..>5368)
                     /locus_tag="VN377_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWR27149.1"
                     /translation="MDKILKSEEILHLITAIGTGIAEEFNIENARYHRIILMTDADVD
                     GEHIRTLLLTFFFRYMRPLIEKGYLYIAQPPLYKMTKGKTVEYAFSEQERVQKAKQMG
                     ETGLGIQRYKGLGEMNPQQLWETTMDPDSRLMLQVTIENAVEADELFTILMGEAVEPR
                     KVFIEQHAKDVVNLDI"
BASE COUNT         1276 a         1263 c         1146 g         1683 t
ORIGIN      
        1 agcggtacac ccaaggagcg ctgatagcaa aaatggagca gctctcactt gggacgaaat
       61 ctactcgtca tgagattatc agtaaattgt attatcgtaa atatgtcata ggggggactc
      121 ctactccaac ctcaacagct atcgctgttg ttgatgcgtt gattaactgt gatgtcgtca
      181 aaccaaagat gaccgccgag cttgaggctg acatgaatga tatcgcagaa gggaagaaaa
      241 cacttgagga gaccgtcaga gaatcacgtt tgatgctgac aaaagtgatg gaagagcttg
      301 agcctgagaa agaaaaaatc aaggaaaaca tcaacaatgc ggtcaaagcg cagaacacca
      361 ttgggccgtg tccgaaatgc ggaaaatccc tcatggtccg ggtgtcaaag aaaggcaaac
      421 ggtttgtcgg ctgtaccgga taccctgagt gtacgaatac ctattcgctt cctcagcaag
      481 gcggcttgac gatgacgacg aaactgtgcg atgcctgtga caccccaatc gttcaggtaa
      541 aaatgaaagg cagaccggca tgggagttat gtctcaattc tgactgcccg aagaaaaaga
      601 aaaaagaaat agaaaaacca cctgctgctc cgacagccta accgatcatg tacgttttgg
      661 ttttttcttc tgaggtttct ttgctttcac cttcttggct ggtttgattt tcttaggttt
      721 tctcaccggt tttgattgtg ccttctttga ctttaccgtc tttgttttct tagctgatct
      781 tcccttggtt ttctttggtt ttttcactgg tttcagggtc actttctttg gcttgctggt
      841 cttgattttc ttcaccgacg caggtttttc cttctttggt tttgcaatct tcatgggttt
      901 cttaggcatc gcttctttct ttacaggctg cttcggttcc aaggtcggtt cctgtttttt
      961 tacttcctgt gctggttctt tctgttcaac acttggtttt ggtgcctcta taggttctgt
     1021 tttttcctca accatgccaa tctgcatccg ttccatggcc tcgacctgtg ccgcttcctt
     1081 gccgttttcc tcaagtatct ttgtgatggt cacggagacg accttgtcgc cctcattgag
     1141 ccgcatgatc cgtacaccca tggtgttcct gccttgttcc ctgatgtccc ggaccggtat
     1201 tcgtacggtc ataccgtgtt ctgtcgtgat gatcaattcg ttttcatcgt tgacctggct
     1261 gacgaacacg acgcttccat tccgctcatt ggtcacaata gtacggactc ctttgcttcc
     1321 tcgatgcgtc atccgatatt catcgatagg tgagcgtttc ccaaaaccgt tctccgtgat
     1381 ggtaaataat gagccggtga tgtcgacaac ggtcatggcg accaccctgt ctttcttgtt
     1441 tttcagccgg ataccgatga cgccgtgcgt ggcccgtccc atgggtcgta gctctcgttc
     1501 attgaaacga catgcttggc caccagcggt tgctatcatc acggtcttgg ttccatcaga
     1561 cagctccacg cccatgagtt catcgccttc ctccagttta atggcgatga ttccgttcac
     1621 tcggatgttc ccgtaggcgg acagcaccgt tttcttaatg gttccttgtt ttgtggtaaa
     1681 aatcaggaaa tgtgtatcgt caaactcatg gatgggaacc gcggtttcaa tcttttcccc
     1741 ttcctcgagt cgcgggagga ggttgatgat ggcttttcct ttagcatgac ggtctccttc
     1801 agggatctta tagcctttca tccagtagac tcgtccgtgg ttggtgaaaa acatcaggta
     1861 atcatgggtg ctggtgataa atgagtcaac gacgaaatct tcttcttttg gccggacgcc
     1921 gattaaccct tttccacccc ggtgctgtgt ccggtaggtt tccgtcggaa tgcgtttgat
     1981 atagccatct ttggtaatgg tgacgacgac ttcttgcaca gggatgaggt cttcgatatc
     2041 gatgcctgct tctccttcaa cgatctgggt gcgtcgcgcg tcaccgaatt tttcttttat
     2101 ctccaggagc tcccgtttga tttccgcaag gatcatctgt ttatctgcaa ggagcgcttc
     2161 aagctcttca atcagctgtt tggtttcatt gtactccttt tcgacagatt cgatttccat
     2221 gccggtgagc ttctgcagcc gcatatcaag gattgctttc gcctggatct ctgagagatt
     2281 aaaacgtttc atcagacggc tttttgcttc ctctacctct ttgctctgtc tgatgatctt
     2341 aatgacctca tcgatgttcc gcagggcaat catgagtcct tcaaggatat gcattttttc
     2401 tcttgctttc ttcagatcat aggcggtacg tcgggtgatg atctcaatgc gatattcaat
     2461 gtaatattgc aggatttctc tgagcatcag gacttttggt tctcctttga caatagcgag
     2521 attcagcaca ccaaaggagg attgcagctc ggtatgttca tacaactgat tcaataccac
     2581 atcatcgata gcatcacgtt tcagctcaat gacgatacgc atgcctttac ggtcgctttc
     2641 atcacgaaga tcagagattc cttcgagttt cttttctttg acaagctcag caatggtctg
     2701 taacaaaaca gatttattca cctggtacgg cagctcatgg acgatgattt tgtttttctg
     2761 ctccccttcc tcaatggagg ttttcgcacg gacgacaagc gagccacgac cagtggaata
     2821 cgcagagaga atcccgccgc gcccgcagat ggtaccaccg gtgggaaaat caggtccctt
     2881 gatgatctcc ataagctcag cagtcgatgt ctcaggagcg tcaatcatcc ggatagtacc
     2941 atcgatgacc tcacagatgt tatgaggcgc catgttcgtg gccataccaa ccgcgattcc
     3001 agaggatccg tttatcaaga gattcggtaa aactgctgga agaacctcag gttcttttaa
     3061 agtaccatca aaattatcac gccactccac ggtttctttc tcgatatcct gcagcataag
     3121 cttggttatc ttggccattt ttgtttctgt attatggttg ataaagccat tcgcaatata
     3181 cgaatgacac gggctgtcaa cacgaaggga atacactgtt ttttcctcac aggtcggtgc
     3241 aatttcgtat atacggttaa agaaatactc ataatgaagg atatcgtcga taagttttct
     3301 atcaactgca tcaagtttct tcatcagaag actgtagttt ctccgcagat tgttataacg
     3361 atcataatta tatcgatgga acaatgggtc cttgtatttg gcacgtaaat actctgcaag
     3421 aaacggaata aaatcgttct tactcatacg tgtcgtgttc aaacttttaa ccagtgctaa
     3481 gcgtttttgt tttttcgaac aggaaaaatc aatgttttcc tggaattttt ttatgttatt
     3541 cacatcagag attatcagct tataacatgg ggttcgttta tctttatagg gtaaggttgt
     3601 aattatccca aaattaagca atacggtctt cagttgatgg atgagttttt tgctatttga
     3661 tagataggtt agctcaatgg attttccacc atgtcgttta tctactttat atgatactga
     3721 gccgtcgcct tcatataacc cgctaaggaa acttgcgatg catccacggg ttgattgtag
     3781 aaccgtgaat ggtatttcct ttttcgcaga agtagtctgc gttaatccaa tattctttaa
     3841 aaattcaacg acatgggcat aatagatact taattcgaca cagtgacatt ttggtaacgt
     3901 acgctcgtag aggttaattc ccgggaactg atgaagaatt gcttttttca ctttgtcgta
     3961 atactcttta ttgaggttat tgaacagtat ttgcttctgg tgaaaactac cttccgatac
     4021 cagagcacct aagagaaacg ctagttcctc atccatcgtt tgtggtaatt ggaagatttt
     4081 cgttctttta ctaaccaacg gatgatattg ccgtaatgaa atattttttt cagggaaaca
     4141 gtgagaggac ctacttaaca ccacataatc attttttgtt atttcagaga gcattttcca
     4201 tttcaggtga ggaataccat gttcattgac ccaggttatt accggatgat tatacgatcc
     4261 ttctaattca tacccttgtt gagtccgcag gtatatcgtc ggatgttttc cagagttaaa
     4321 aaatttcgat gcatgagttt tttggttctg ataattgaca atttgcacat tgatttccgt
     4381 ctcttcgcca ggcgccatgt ctttaatgtc tacgatccct tgatcggtca agaccatggc
     4441 atcacctgta acacaatatc tcatagctgc cgcgctgtca ccatcgactg aaccaaaatt
     4501 accttgtccc gcaatcaagg gatatcgtaa ggagaaatcc tgggccattc ggactaacga
     4561 atcgtacacc gcttggtcac catgcgggtg gtacttcccg agcacttctc cgaccactcg
     4621 ggcggatttc ttatatgctt tttcatagcc catccccatc tcgttcattg cgtacaagat
     4681 ccgtcgatgt acgggtttga gtccatcacg gatgtccggg agagcccggc tcataatcac
     4741 actcatcgaa taatcaataa atgctcgttt catctcttcg ttaatatcgc gtggttgatc
     4801 gtttttcttg ttaatctcag ccatctttca tcacctcaga tatccagatt cactacatct
     4861 ttggcatgct gttcaataaa cacttttcgt ggttctacag cttcacccat cagaatcgta
     4921 aataattcat cagcctcgac agcattttca atcgtcacct gcaacatcaa acggctgtca
     4981 ggatccatgg ttgtttccca cagctgctgc gggttcatct caccaaggcc cttgtagcgt
     5041 tgaataccca gtccggtttc ccccatctgc tttgctttct gcactctttc ctgttctgaa
     5101 aacgcgtatt ccaccgtctt tcctttcgtc attttataga gtggtggttg tgcaatgtac
     5161 aaatatcctt tctcaatcaa cggtcgcatg taacggaaga aaaaggtgag taacaaggtt
     5221 cgaatatgct caccatcgac gtcagcatcg gtcatcagaa tgatccgatg ataccgagcg
     5281 ttctcaatgt tgaactcctc agcaataccg gtgccaatcg cggtaatcag atgaaggatt
     5341 tcttcgcttt tcaggatctt atccatcc
//
LOCUS       DATMXJ010000061         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_48273, whole genome shotgun sequence.
ACCESSION   DATMXJ010000061 DATMXJ010000000
VERSION     DATMXJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48273"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..484
                     /locus_tag="VN377_01810"
     CDS             149..484
                     /locus_tag="VN377_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWR27150.1"
                     /translation="MAEKKQARDIGLNVKPPSDSCTDKYCPFHGTLPVRGQVLMGTVV
                     STKRQNTVSVKREFMHYVKKYERYEKRTSTYHAHCPPCMNLTAGDTVRIAECRPLSKT
                     VSFVTIEKV"
     gene            500..898
                     /locus_tag="VN377_01815"
     CDS             500..898
                     /locus_tag="VN377_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWR27151.1"
                     /translation="MKGIAGRVMRGLPVGARLTCIDNTGAKVVQIIMTVKIKGVHRRT
                     PTAGVGDLVIVSVKKGATEMRRQILKAVIVRQRRPYRRPDGLMVQFEDNAAVLITDTG
                     ETKGTMIKGPVAIEAAERWPKIAALAATIL"
     gene            909..1571
                     /gene="rplX"
                     /locus_tag="VN377_01820"
     CDS             909..1571
                     /gene="rplX"
                     /locus_tag="VN377_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWR27152.1"
                     /translation="MTSTKPRKQRYMLYNAPLHKKRKWISSHLAENLLLKYDRRSLPV
                     VKGDTVKVMRGNYRGHEDKISKVNVRDQTVEIEGATITTAKGTKIAKPIHASTLLITK
                     LNVTDKWRRRKLESGLSETAKKEIEREAAEQLKALEAEKKAAEEATKAEEKHEEEIKE
                     APQEKETTEPTEEAIEPIKKEEIPQEKVDDTSAEPPKKKPTTKKTPTKKTTKKNKKEG
                     ET"
     gene            1568..2284
                     /locus_tag="VN377_01825"
     CDS             1568..2284
                     /locus_tag="VN377_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HWR27153.1"
                     /translation="MSKHLKRLAAPRVLKLHRKERALTIRAAPGPHPLEYAIPLGLLV
                     RDYLGLCDTYKEARKIVSNGDILVDGVKRKNFKFPCGFMDVISIPKMRKYIRILYDRN
                     GKLTLIPISESDAEWKLCRIQNKTIIKGKKIQLNFHDGKNKLIEKDEYKTGDVLKIAF
                     KENKIDEVYKFDKGTVSMIIGGTHIGEIASIEEIHTVASSKPNLVNLKGEKEFSTIAH
                     HVFPIGKTKPAITLPEVKMQ"
     gene            2281..2871
                     /locus_tag="VN377_01830"
     CDS             2281..2871
                     /locus_tag="VN377_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWR27154.1"
                     /translation="MSAKKTTTTKTHKTATRSKKSASKKKEAEHPMKPHVAKVTVNIG
                     VGEAGEKLKKAESVLQGVTKQKPVQTLSKTTNKDWGLRKFMPIGCKVTLRKKAAETFL
                     ISALKIRENKISEYSFDNQGNFSFGVPDHTLFEGQKYDPNIGIFGMDICVTVENPGYR
                     IKHRRIDRRKIPRRHELSREETMKFITEKFDVEVVE"
     gene            2868..3023
                     /locus_tag="VN377_01835"
     CDS             2868..3023
                     /locus_tag="VN377_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HWR27155.1"
                     /translation="MRIKERKKMYGRKIGCEQCGRKRGIIRRYGLHLCRQCFRDKAEE
                     LGFKKYD"
     gene            3039..3428
                     /locus_tag="VN377_01840"
     CDS             3039..3428
                     /locus_tag="VN377_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWR27156.1"
                     /translation="MLNDPLADALSIIRNAETRGIHKCIIRPSSKLVGGVLNLLKEKG
                     YLGNFEYVEDGKAGIYQVELLGSINNCGVIKPRYPIKVDEMDKWEARYLPARDFGLLI
                     LTTTEGIIGHDEARKKGIGGKLLAYVY"
     gene            3441..3980
                     /locus_tag="VN377_01845"
     CDS             3441..3980
                     /locus_tag="VN377_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWR27157.1"
                     /translation="MEKKATIKEEIKIPDGIQITIEGKTVHVKGQKGSMSKILSHPKI
                     QLTVSGTVVQISCSQSPRRKEKALIGTFKAHVKNMMKGVSEGYECEMKTVFSHFPIKT
                     SVEGNHLIIQNFLGERFPRRAEILENVKVDIKGEHIILTAIDKEKVGQTAANIERATK
                     VKNRDIRVFQDGIYIVKRG"
     gene            3984..>4321
                     /locus_tag="VN377_01850"
     CDS             3984..>4321
                     /locus_tag="VN377_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWR27158.1"
                     /translation="MTKETVLKEFEALKGVGKVKAKLLYDNGYDSLEKLKKAKIKDLT
                     KLKGITEKNAKEILDQLKEKKGKTAKKTDETKEPKDQKTESVEIVDQKEGAYQPKIKP
                     QLNDREKEQLV"
BASE COUNT         1632 a          876 c          974 g          839 t
ORIGIN      
        1 tcctcataga aatcgaaaaa aaacaaaaac gaatagcaaa gcaaaccgca acgtttgcgt
       61 ttgaatatca tggtaaaaaa accatcgtga aaggatcacg attggtgtat cgacctgaag
      121 atagaataaa aaaaacaagg tgaacacatt ggcagaaaaa aagcaagcac gagacatagg
      181 gttgaatgtc aaaccaccca gcgatagctg taccgataag tattgcccgt ttcatgggac
      241 gttacccgta cgaggacagg ttctcatggg aacggtggta tcaaccaaaa ggcaaaacac
      301 cgtctccgtc aagcgagaat tcatgcatta tgtaaagaaa tacgagcggt atgaaaaacg
      361 aacctcaacg tatcatgctc actgtccgcc ttgtatgaat ctgacagcag gtgacaccgt
      421 tcgaattgca gaatgtcgac ccttaagtaa aacagtttcg ttcgtcacaa ttgaaaaagt
      481 gtaagattgg tgagaagaca tgaaaggaat agcgggtaga gtaatgcgag ggttgccggt
      541 tggcgcccgt ttaacatgta tcgataacac tggtgcaaaa gtcgttcaaa tcatcatgac
      601 tgtaaagatt aaaggagttc atcgtcgaac cccaaccgct ggcgtcggtg atttggtaat
      661 cgtcagcgta aaaaaaggag caacggagat gagacggcag atcttaaaag cagtaatcgt
      721 ccgtcagaga cgcccgtacc gacgcccaga tggtctcatg gttcaatttg aggataatgc
      781 tgcagtatta attacagata ctggagaaac aaaaggaacg atgattaaag gccctgtcgc
      841 catagaagcg gcagaacgat ggccaaagat agctgcattg gcagcgacaa ttctctgaag
      901 tgagtgatat gacatctaca aaaccaagaa aacaacgata catgctctat aacgcacctc
      961 ttcataaaaa gagaaaatgg atttcttcgc atctcgcgga aaacctcctg ttgaaatatg
     1021 accgacggag tctaccagta gtgaaaggag acacggtaaa agtcatgcgt ggaaactacc
     1081 gggggcatga ggataaaatc tcaaaagtaa acgtacgaga ccagacggta gaaattgaag
     1141 gcgccaccat caccactgcg aaaggaacaa agatagccaa accaatccac gcaagcacgt
     1201 tgctcattac gaaattaaat gttactgata aatggcgacg acggaaactc gaaagcggac
     1261 tttcagagac ggcgaagaaa gaaatcgaaa gagaagcagc agagcaactt aaagctcttg
     1321 aggctgaaaa gaaagcagca gaggaagcaa caaaagctga agaaaaacat gaggaagaga
     1381 taaaagaagc tcctcaagaa aaggaaacga cagagccaac cgaagaagcg atagaaccaa
     1441 tcaaaaaaga agaaatacca caagaaaaag tcgacgatac cagcgctgaa ccgccaaaaa
     1501 agaaaccaac gacaaaaaaa actcccacca aaaaaaccac aaaaaagaat aaaaaagaag
     1561 gtgaaacatg agcaaacacc tgaaaagact cgcagcacca cgagtattaa aactccatag
     1621 aaaagaacgt gcactaacga ttcgcgccgc accaggccct catccgttag aatacgccat
     1681 tccccttgga ttactcgtac gagattacct tggtttatgc gacacgtata aagaagcacg
     1741 aaaaatcgta tccaatggag atatcctcgt tgatggagtg aaacgtaaga acttcaaatt
     1801 cccctgtgga ttcatggatg ttatctctat accaaaaatg cgcaagtata ttcgaatcct
     1861 ctacgataga aatggaaaac tcacgcttat ccccatatcg gaaagcgatg cagaatggaa
     1921 actctgccga atacaaaata agacaataat caaaggaaaa aaaattcagc tcaattttca
     1981 tgatggaaaa aataaactca tcgagaaaga cgaatacaaa actggagatg tcctgaaaat
     2041 cgcattcaag gaaaataaga tcgacgaagt ctataagttt gacaaaggaa ccgtctcaat
     2101 gatcatcggt gggacgcata tcggagaaat cgccagtatc gaagaaatcc atactgtcgc
     2161 ctcctcaaag ccaaatctcg tcaatctgaa aggagaaaaa gaattctcca ctatcgcaca
     2221 ccacgtgttc cccattggga agaccaaacc tgctattacg cttcctgagg tgaagatgca
     2281 atgagcgcaa aaaagacaac cacaacgaaa actcataaaa cagcgacgcg ttcaaaaaaa
     2341 tccgcttcca agaaaaaaga agcagaacat ccgatgaagc cacatgtcgc aaaagtgacg
     2401 gtgaatatcg gtgttggcga agcaggtgag aaactcaaga aagcagaatc agtcttacaa
     2461 ggagtcacga aacaaaaacc agtgcaaaca ctctcaaaaa caacgaataa agactggggt
     2521 ctacgtaaat tcatgccaat aggctgcaaa gtcaccttac gaaagaaggc cgccgagacg
     2581 tttctaatca gtgcattaaa aataagggag aataaaatct cagagtattc atttgacaat
     2641 caagggaatt tctcgtttgg agtgccagat cacacactct tcgaaggaca aaaatacgac
     2701 ccgaacatcg gtatcttcgg gatggatatc tgcgtcactg tcgaaaatcc tgggtatcgg
     2761 attaaacatc gtcggattga ccggcggaaa ataccgcgac gccatgaact cagcagagaa
     2821 gaaaccatga aatttatcac ggaaaaattt gacgtagagg tcgtagaatg aggattaaag
     2881 agcgaaagaa aatgtacggc aggaaaatcg gatgtgagca atgtggcaga aaacgcggga
     2941 ttatacgacg ctacgggctt catctttgca gacaatgttt ccgtgacaaa gcagaagaat
     3001 tgggttttaa aaaatacgat tagaggttgt tgaagattat gttaaacgat ccattggcag
     3061 atgcattatc aatcatcaga aacgcagaga cccgaggaat acataaatgc attatccgcc
     3121 cctcatcaaa actcgtcggc ggagtcctaa acctcctgaa agaaaaaggg taccttggga
     3181 atttcgagta tgttgaggac ggaaaagcag gtatttatca agtggaactc ctcggaagca
     3241 tcaataactg cggtgtcatt aaaccacgct atcctatcaa agtagatgag atggataaat
     3301 gggaggctcg gtatctccct gcgcgggatt tcgggttact tatccttacc accaccgagg
     3361 gtatcatcgg tcatgacgag gcacgtaaga aaggcatcgg aggaaaactc ctcgcgtatg
     3421 tatactaaag agtgagacgc atggaaaaga aagcgacaat aaaagaagaa atcaagatcc
     3481 ctgacggcat acagattact atcgagggaa aaactgttca tgtgaaggga caaaaaggat
     3541 cgatgtcgaa aatcctctcc catcccaaga tacaattaac ggtgagcggt accgttgttc
     3601 agatctcatg ttcccagtct cctcgtcgga aagaaaaagc gttgattggg acctttaaag
     3661 cacatgtcaa gaacatgatg aagggcgtca gcgagggata tgaatgtgag atgaaaaccg
     3721 tgttttctca ttttcctatc aaaaccagcg tcgaagggaa ccaccttatc attcagaact
     3781 tccttggaga acgattcccc agacgagcag agatccttga aaacgtcaaa gtggacatca
     3841 aaggagaaca tatcatcctt actgccatag acaaagagaa agtaggacag acggctgcca
     3901 atatcgaacg tgcgaccaaa gtaaaaaata gagatatccg ggtgtttcaa gatggtatct
     3961 acattgtgaa aagaggctaa accatgacaa aagaaacagt gttaaaggag ttcgaagcgt
     4021 taaaaggtgt tgggaaagta aaagcaaaac tcctctatga caacggatat gattccctgg
     4081 agaaattgaa aaaagcaaaa ataaaagatt taaccaaact caaagggatc actgagaaga
     4141 acgcaaagga gattcttgac cagctcaaag agaaaaaagg aaagaccgca aaaaaaacag
     4201 acgaaacgaa agaaccaaaa gaccagaaaa cagaatctgt ggaaatcgtt gatcagaaag
     4261 aaggggcata ccagccaaag attaaaccac agttaaatga cagggaaaaa gagcaactgg
     4321 t
//
LOCUS       DATMXJ010000062         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_11617, whole genome shotgun sequence.
ACCESSION   DATMXJ010000062 DATMXJ010000000
VERSION     DATMXJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11617"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="glgP"
                     /locus_tag="VN377_01855"
     CDS             <1..803
                     /gene="glgP"
                     /locus_tag="VN377_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWR27159.1"
                     /translation="SEPFQRVFDKYMPGWHTDPYILRGAFGIDKTDIWAAHMEAKKKL
                     IDFVNNRYNVGMNYDHFTIGFARRQTAYKRPELLISDAERLMSIAEKVGPIQIIYAGK
                     AHPKDGSGKETIKRIFSAMKNIRGKVKMAYIHNYDIAIGKLMTSGVDLWLNTPRRPQE
                     ASGTSGMKAAHNGIPQLSTLDGWWIEGRVENITGWAIGTRKSNERESDDENDQADLYN
                     KLETWIIPKFYNDRDNWIRTMRSCIAINASFFNTNRMIEQYVLNAYFM"
     gene            complement(783..2645)
                     /locus_tag="VN377_01860"
     CDS             complement(783..2645)
                     /locus_tag="VN377_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWR27160.1"
                     /translation="MREWIVTNGLGSYASLTHSGEATSKFHGLLVASLEPPTKRWMFV
                     SNVLDRIQIKDHVYPLKGHKCKFVFDYFPSLVYNIEGVHLKKTFFMDYGKNTSIIKYT
                     IDTHKPLVLSHIPVVNSRHFYDMTSPGSASFSQNVIDQGVIVTLDNINKKLKILLKNS
                     CYLPDGYWEEFFYKKDKERNDSWRDHNFHVGEFQVPLKQSAEYYLMFTIEDESRDDPS
                     LIYDREIQRKQRLLTQAMLPHACDKLVLSADTFVVKKGSGKSIVAGYHWFSDWGRDTL
                     IALPGITLVTKRFDDAKRIIESYGHYSRNGLIPNVFTERDSQPVYNTVDASLWYIDRA
                     YQYLKYTNDVRLVEKLWPIMQSIIERYKNGTDFGIHMDNDFLISHGEGLTWMDVKIGD
                     SYITPRSKKAVEIQALWYNALRIMSTLSSLLGKDNHYSALADKVKESFRRQYVHPYDV
                     IDTKDLSMRPNQIFLVSLDFSMIERQMQKSIVAELQKSLVTIFGLRTLSPQDSRYKGT
                     YLGNHHRDFAYHNGTVWPWLLGQFIKAYVKVHDRDPAQRQYAFQNFLQPMLDVYGDSW
                     DGSIYEIFDGDPPFAPHGCITQAWSVAEILRTWVEDIENIAPRYESLLLHEVGV"
     gene            complement(2691..>3303)
                     /locus_tag="VN377_01865"
     CDS             complement(2691..>3303)
                     /locus_tag="VN377_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWR27161.1"
                     /translation="SNEDRLVLRRGYGVQDHETLLFFIGRLVPVKGIDKLVRAFPLVL
                     KDFPNTKLLILGIGDMEHEIRSIADGLGIHDKVIMRAEFVSEEERIRHYAAADVVVLP
                     SLYEPFGIVCTEALSMAKPTVVGAKGTNGMREQVIPSGEKQCGIHVNPFEPKDIAWGI
                     IQVLQSPDKGLQWGQNGRQRVLEEFSWDAVTKRTVAIYKEFMR"
BASE COUNT          961 a          731 c          722 g          889 t
ORIGIN      
        1 tctctgaacc attccaacgt gtgtttgata aatacatgcc cggctggcat actgatccct
       61 atatactgcg cggagcattt ggtatcgaca aaacagatat ctgggctgca cacatggaag
      121 caaaaaagaa gcttattgat tttgtgaaca ataggtataa tgtagggatg aactacgatc
      181 atttcaccat cgggtttgcc aggagacaga ctgcgtacaa gagaccagaa ttattgatat
      241 ctgatgcaga aagactcatg tcgattgcag aaaaagtagg gcccatacaa attatttacg
      301 cagggaaagc acatccaaaa gacgggtcag gaaaagagac gataaaaagg attttttcag
      361 cgatgaaaaa catcagagga aaagtaaaaa tggcctatat ccacaattat gatatcgcga
      421 tcgggaaact catgacctca ggggttgatc tctggttgaa cacccccaga cgacctcagg
      481 aggcctcagg gacgtccggg atgaaagcag cccataacgg gatcccgcaa ttaagcaccc
      541 tggatggttg gtggatcgaa ggacgcgtcg aaaacatcac agggtgggcg atcggaacaa
      601 gaaaatcaaa tgagcgggaa tccgacgatg agaacgacca ggctgatctg tacaataaac
      661 tggagacatg gatcataccg aagttctaca acgatcgaga taactggatt cgaaccatga
      721 gaagctgcat tgcgatcaat gcatcgttct tcaatacaaa tcgaatgatc gaacagtatg
      781 tgttaaacgc ctacttcatg taataagaga gactcgtatc gtggtgcgat attctcaata
      841 tcttcaaccc aggtacggag tatttctgcg acactccatg cttgtgtgat gcaaccatga
      901 ggtgcaaatg ggggatcccc atcaaagatt tcataaatag aaccatccca ggaatcccca
      961 tagacgtcca gcatcggttg taagaaattt tggaatgcgt actgtctttg tgccgggtca
     1021 cggtcatgta cttttacata tgccttaatg aattgtccca gcaaccatgg ccacaccgtt
     1081 ccattatgat acgcaaaatc cctatgatga tttccaagat aggtgccttt atagcgagaa
     1141 tcctgaggag agagcgtccg caaaccaaag attgtgacta atgatttctg taattcagcg
     1201 acaatagatt tctgcatctg tctctcaatc atggaaaaat caagagacac aaggaaaatt
     1261 tggttcggtc gcatcgaaag atctttggta tcaatcacat cataggggtg aacatactga
     1321 cgtctgaaac tctccttgac cttgtctgct aacgcactat aatgattatc tttccctaag
     1381 agagaggaaa gagtgctcat gattcgtaaa gcgttatacc ataacgcctg gatctccact
     1441 gccttctttg accgtggagt gatataagaa tccccgattt tgacgtccat ccaggtgaga
     1501 ccctcaccat ggctgataag aaaatcatta tccatgtgaa taccgaaatc tgtaccattc
     1561 ttgtatcgct cgatgatcga ttgcatgatc ggccagagtt tctcaacgag acgtacatca
     1621 ttggtgtatt tgaggtattg ataggcacgg tctatgtacc aaagtgatgc atcaaccgta
     1681 ttgtacaccg gttgagaatc tcgctctgtg aacacattag ggattaaccc attcctgcta
     1741 taatgcccgt aactttcaat gattcgtttt gcgtcatcga atcgtttcgt caccaaggtg
     1801 attcctggaa gagcaatcaa ggtatcacgc ccccagtcac tgaaccaatg atatccggcg
     1861 acaatggatt tcccagagcc tttctttaca acaaaggtat cagcggataa cacaagttta
     1921 tcgcatgcgt gcggcaacat cgcctgcgtc agcaatcgtt gtttccgttg aatctcccga
     1981 tcatagataa gtgacgggtc atctctggat tcatcttcga tggtgaacat cagataatat
     2041 tcagcggatt gtttgagcgg cacctgaaat tcaccgacat gaaaattatg gtccctccaa
     2101 gaatcgtttc gttctttatc ttttttatag aaaaattctt cccagtatcc atcaggcaga
     2161 tagcaggaat ttttcaggag tattttcagc ttcttgttga tattatcaag ggtgacgatc
     2221 accccttgat cgatcacgtt ctgagaaaac gatgcagatc ctggcgaggt catatcgtag
     2281 aaatgtcggg aattgaccac agggatgtga gaaagaacca atggtttatg cgtatcaatc
     2341 gtgtatttga tgatagaagt attttttcca taatccatga agaaagtttt tttcagatgc
     2401 accccttcga tgttgtaaac aagtgaagga aaataatcga agacgaattt acatttgtga
     2461 cccttcagtg gatagacatg gtccttgatc tgaatcctat ccagaacatt ggagacaaac
     2521 atccatcgtt tggtgggagg ttccagactt gcaacgagca atccatggaa cttacttgtg
     2581 gcttcaccgg aatgcgtcaa ggatgcgtaa cttccaagtc catttgtgac aatccattct
     2641 ctcataagta gctccaacca aaaatgtgta tacgatatcg ttgaaaaaac ttatcgcatg
     2701 aattctttgt aaatggcgac agtccgtttt gtcacagcat cccaactgaa ttcttctaag
     2761 acgcgttgcc gaccgttttg tccccattgc agacctttat ccggtgattg cagaacctgg
     2821 atgatccccc aggcgatatc ctttggttca aacgggttga catgtatacc gcattgtttc
     2881 tcaccagatg gaatcacctg ctctcgcatc ccattggttc ctttggctcc gacaaccgtt
     2941 ggttttgcca tggacaaggc ttctgtacag acgattccaa atggttcgta gagcgatggg
     3001 agaactacaa catcagcagc agcgtagtga cggattcttt cttcttcact gacaaactct
     3061 gctctcataa tgaccttgtc atggatccct aatccatctg cgatggatcg gatctcatgc
     3121 tccatatcac cgatgccaag gatgagtaat tttgtgttgg ggaaatcttt gaggacaaga
     3181 gggaatgctc gcaccagttt atcaattcct ttcactggta ccaaacgacc gatgaaaaac
     3241 aataatgttt cgtgatcttg tacgccatag cctcgtcgta acacgagtcg atcttcattc
     3301 gat
//
LOCUS       DATMXJ010000063         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53525, whole genome shotgun sequence.
ACCESSION   DATMXJ010000063 DATMXJ010000000
VERSION     DATMXJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53525"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1112
                     /locus_tag="VN377_01870"
     CDS             207..1112
                     /locus_tag="VN377_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27162.1"
                     /translation="MMKKLRAIWELMRLEHGVMLFLAILIGSLISQKTLFNNLNLPLL
                     NIVLTFFTALFLEASTFALNDYYDLEIDRINKRTDRPLVRGDLSPKTALNIFYLLFPL
                     GLICSYFVNMTCFIIALITALISVLYDVKLKKIKLIGNFYIAYIMAIPFVFGGATVLG
                     NNAFSLGTINPAIYVISLIAFLTGAGREIMKDVMDFEGDKRLGVKSFPRYIGTRTSNG
                     VASLFYLCAIALSFLPYFSVSFGGYYKNITYFILVFITDTMLLSTCLQLMFKKQPNMK
                     FHRKYTLVAIFIGLLAFLVGAFIGG"
     gene            1119..1787
                     /locus_tag="VN377_01875"
     CDS             1119..1787
                     /locus_tag="VN377_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HWR27163.1"
                     /translation="MYQSDLLGVAAVYIYVIALIVFTEKVFSKKYPVQSRKFLHIMTG
                     NIAFILPLFQTREIMAFVAAGPFILFTFLMSPYSPIKSMRGKTSEAGHGLGLVYYAIT
                     WTVLAYVFFDHREIIAMGILAMSYGDGLASWIGIKYGERKYTVFKDTKSYVGSITMFV
                     CTFLLLIIALLFYSVPVTGRVVAYLLCIAGVATVVEGITPLGLDNLSVPFVTAVMYWF
                     FLVG"
     gene            1789..2370
                     /locus_tag="VN377_01880"
     CDS             1789..2370
                     /locus_tag="VN377_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HWR27164.1"
                     /translation="MRLVIVDGLDGVGKDTHAQLIKQRYEKKGEKVIVRSHPESDNFY
                     GRTAKKALLGQGKINRLKASVFYAMDVMYSLRRYYRKPTSDTLIMVRYLMGTAYLPRR
                     LTRTGYRFFEKLVPTSSYMFFLDANPEELVERIKQRSETEMFETQEAFLKVRSKALDL
                     AKGWYIINTGQSIEQTYSAIEKILDKLDDASAS"
     gene            complement(2405..2827)
                     /locus_tag="VN377_01885"
     CDS             complement(2405..2827)
                     /locus_tag="VN377_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX pyrophosphatase"
                     /protein_id="HWR27165.1"
                     /translation="MPQVVTCILEHDGKILLLKRSNQVGTYRGLWGGVAGYVEELEEP
                     YDTAIKEIRQEAGIEVDALELVRKGNPIEFSDTYDGRRYNWIVYPFLFHIQSKELVHI
                     DWEHEEYRWVHPSEVRKLETVPCLDEVITQLLGTIDIL"
     gene            complement(2846..>3034)
                     /locus_tag="VN377_01890"
     CDS             complement(2846..>3034)
                     /locus_tag="VN377_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HWR27166.1"
                     /translation="HLVPLVPPRLLLFGSIPPKRMVNITRAYVTVFFEVILKGEPIET
                     LLNLSAQFDEVQFDYKVG"
BASE COUNT          804 a          670 c          670 g          890 t
ORIGIN      
        1 tattactaca gccatagatc tcatcacttc aaagaaagta acggtcgtgg acatgatatc
       61 ccatagcctt cctcttactg aagcagcaaa aggctttcag ctcgtcgcaa aagcaaaaga
      121 ttccatgaaa gtgattttaa aaccataaaa aaaatattca atcttcatta ttaaaaagtt
      181 tataacactg cattttattc tcttatatga tgaaaaaact cagagccatc tgggaactca
      241 tgcggctaga acacggagtg atgctgtttc tagctatcct catcggctcc cttatttctc
      301 aaaaaacact ttttaacaat ctgaatctgc cgcttctgaa tatcgtcctt acgttcttta
      361 ccgctctttt tttagaagca agtacctttg cattgaacga ctactatgat ctggagatcg
      421 accggataaa caaacgaaca gaccgaccat tggtccgagg tgatttatcc ccaaaaacag
      481 cgttaaacat tttttatctt ctgtttccat tggggctcat ctgctcgtat tttgtgaaca
      541 tgacgtgttt tatcatcgcc ctcattactg ccttgatatc ggtcttatat gatgtgaaat
      601 tgaagaaaat caaactcatt ggtaattttt acatcgcgta catcatggcg atacccttcg
      661 tcttcggagg agcgactgtt cttgggaaca atgcattttc acttggcacg atcaatccag
      721 caatttatgt tatctccctt atcgcatttc ttactggagc tggtcgagaa atcatgaagg
      781 atgtcatgga tttcgaagga gataaaagat taggcgtcaa atcgttcccg cggtacatcg
      841 gtacccgtac ctcaaatggt gttgcatcac tcttctatct ctgtgccatc gcattgagct
      901 tcctgccgta tttttcagta agtttcgggg ggtattataa gaacataacc tattttatcc
      961 tggtgtttat cacagatacg atgttactca gtacatgcct tcaactgatg ttcaagaaac
     1021 agccgaacat gaagtttcat aggaaataca cacttgtcgc aatcttcatc gggcttcttg
     1081 cgttccttgt tggagcattt atcggaggat gaagaaccat gtatcaaagc gatttacttg
     1141 gggttgccgc ggtctatatc tatgttattg cactcatcgt atttaccgaa aaagtgtttt
     1201 caaagaaata cccggtgcag agcaggaaat tcctgcatat catgaccggg aatatcgcat
     1261 ttatcttacc cttatttcag accagggaga tcatggcgtt tgtcgctgct ggtccgttca
     1321 tcctgtttac gtttctcatg agtccgtact caccgatcaa gtcaatgcgg gggaaaacct
     1381 ctgaagcagg acatgggctg ggactggtgt actatgcgat cacctggacg gtgcttgcat
     1441 acgtgttttt cgatcatcga gagattatcg cgatggggat ccttgcgatg tcttacgggg
     1501 atggtctggc atcatggatc gggataaaat atggggaacg gaaatacacg gttttcaaag
     1561 acacgaaaag ctatgtcgga tcgataacga tgtttgtctg tacgtttttg ttactgatta
     1621 ttgctctgtt attttattcg gttccagtca ccggaagagt tgtggcgtat ttgctttgca
     1681 tagcaggggt cgctacggtc gtcgaaggga ttacaccgct tggtcttgat aacctctcag
     1741 taccgtttgt caccgctgtc atgtactggt tctttttggt ggggtgacat gcgactcgtc
     1801 atcgtggatg gccttgatgg agttggcaaa gacacccatg cacagcttat caaacaacgg
     1861 tatgagaaaa aaggggaaaa agtgatcgtg cggtcccatc cagagtcaga taatttctac
     1921 gggcggaccg cgaagaaagc attgcttggc caagggaaga tcaaccgact gaaggcatca
     1981 gtgttctatg cgatggatgt catgtattcg ctacggcgat actatcggaa gccgacgagt
     2041 gataccctga ttatggtgcg gtatctgatg gggacagcgt acctgccgcg acgtctgaca
     2101 agaaccgggt atcggttttt tgagaaactc gtgccaactt catcgtacat gtttttctta
     2161 gatgccaatc cagaggaact cgtcgaaaga attaaacaac gtagtgaaac tgaaatgttt
     2221 gagacacagg aggcgttcct gaaggtccga tcgaaagcat tggatcttgc taaaggttgg
     2281 tatattatca acaccggtca gtcgattgaa caaacctata gtgcgattga aaaaatattg
     2341 gacaagcttg acgacgcatc ggcttcatag aaagtatttt gttgtttctg cttctttttt
     2401 ttgattataa aatatcaatg gtcccaagta gctgtgtaat gacctcatca agacaaggga
     2461 cggtttcaag ttttctcacc tctgagggat gcacccagcg gtactcttca tgttcccagt
     2521 caatatgaac gagttctttg gattgaatat ggaacagaaa tgggtacacg atccagttgt
     2581 atcgtctccc gtcataggta tcggaaaatt cgatggggtt tcctttccga acgagttcta
     2641 atgcatctac ctcaatccct gcttcctgcc ggatctcttt tatcgcggta tcatagggtt
     2701 cctccagctc ctcgacatat ccagcgacac cgccccacaa accacggtat gtccctacct
     2761 ggttacttcg tttcaaaagc aggatcttcc catcatgctc taaaatacat gtcactacct
     2821 gcggcattat tcaacaactc ctctttcatc ccaccttgta gtcgaattgc acctcatcga
     2881 actgagcaga gaggttcagc aacgtctcaa tcggttctcc tttcaggatc acttcaaaga
     2941 acacggtcac gtaggctctg gtgatgttca ccatgcgttt cggaggtatc gatccaaata
     3001 agagaagcct tggtggaact aagggaacca gatg
//
LOCUS       DATMXJ010000064         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_43134, whole genome shotgun sequence.
ACCESSION   DATMXJ010000064 DATMXJ010000000
VERSION     DATMXJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43134"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..423)
                     /gene="sepF"
                     /locus_tag="VN377_01895"
     CDS             complement(55..423)
                     /gene="sepF"
                     /locus_tag="VN377_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HWR27167.1"
                     /translation="MGITSKIFGEKKTSTTEGYIDLEKYADAQVGGMTGARMRVAIGD
                     IQRYEDLKDLTDFVYGGNVLILDFTTISDQEVLLKRVTNELKRITDDIGGDVAGIGNN
                     LMVVSPNGVKVERRKLHGKF"
     gene            757..3363
                     /locus_tag="VN377_01900"
     CDS             757..3363
                     /locus_tag="VN377_01900"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27168.1"
                     /translation="MKKKVIDLFVCSLLIATIPTVGALNGGGLQPTTRSSYPLELAHW
                     NQIQQLLVSDDIASDLVVDTSLLRDITLYDDYSVNNLKWPGYETLGDRDNQSPVFGTP
                     SPDNDSHNISLSFTWSISINDTEGDSFTWTIECDNGQKNEEKGDVNGTKSLEISNLLY
                     KTSYTVWVNATDEDGSGNYTKKWYIFNTKDTLPPVFGNPDPENGSTNNPLSFIWSIPI
                     NDSEGDEFSWSIQCSNGDVSSGSNELNGTKILVLSSLTFSTTYTVWVNATDPDGSGVF
                     TRRWYTLTTKDNQPPIFGLPNPSNESINNPINFIWNISIIDSEGNTFNWTIQCNNGQF
                     NSGTDEDNGTKLLALSNLAYSTTYIIWVNATDATGSGLWNRGWYTFTTEQENHSPDFG
                     APSPTNGSANNPINLSWSILISDSEGDTFTWTIQCNNGQSNNSVVGEANGTKTLILSN
                     LANATTYTVWVNATDSGGSGVYTRRWYTFTTKSNQPPIFGSATPSNGSSDRPLSFTWM
                     IPINDLDGNSYDWTIQCSNGQTSSGMTANNGTKTLALSGLAYETTYRVWVNATDPGGS
                     GLYTRRWYTFRTRNVGGTPPEEPPESSNNNPIADASAREPYQGLINTTILFDGSLSYD
                     PDGNITTWLWDFDDNTKVNGMTVYHSFSEAGIYSVTLTVTDNDGASNTDTTTCVISEH
                     NQPPTIPKIDGPTSGTKNTLYTYTASSTDPDNDRLQYSFLWEGSISQPSGFIPSGINY
                     SVNHRWMNAGRYNLTVTVTDNQTESSSKITIYIDAVQTRGAGYLLDLDGDGIYDAFYS
                     DETHQTVSIQRNGDSFLIDKDGDGHWEYVYNATYGLTSYQEPRKTPGYELGFTLCAIA
                     VVILLTRKRKTI"
     gene            3385..3645
                     /locus_tag="VN377_01905"
     CDS             3385..3645
                     /locus_tag="VN377_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27169.1"
                     /translation="MGLRLNENGRLVERITLKIKMFDTGKWEWTVRQRFIKEPRIKLH
                     DMLTILDEVKAEIIASEETEKQDEMTQEDAIPATQPETPKTS"
     gene            3713..4249
                     /locus_tag="VN377_01910"
     CDS             3713..4249
                     /locus_tag="VN377_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HWR27170.1"
                     /translation="MLYYIHGYQSSPSGEKAALLKATLHAIPIAYRESVPEELVISEC
                     LSRISEAIANDQQVILIGSSLGGFLAASTALTHSTVKQLILLNPAIIPSELDLNTIEG
                     MPLRILQEMRDPRLFQQKIKATITILRGTLDDIVPDEWILTFAQAQNATIRLYTDDHR
                     FSKNMSRLPEIISELIQQ"
     gene            complement(4262..4750)
                     /locus_tag="VN377_01915"
     CDS             complement(4262..4750)
                     /locus_tag="VN377_01915"
                     /inference="COORDINATES: protein motif:HMM:NF011153.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HWR27171.1"
                     /translation="MVVKIKNRHRLKERDVKELSADLQTRFGDDFLKGKVGVDIGSVE
                     EFTVLLVDDSMDFMMHNNKFLFTLQGLMKHQPKAKYVVIDMGAISYITKGADVMAPGI
                     SDVDVMIQKDDFVWICDEKHRKPLATGIALMSGEEMKTKKPGKAVKTLQYVGDRFWTL
                     TH"
     gene            4852..5070
                     /locus_tag="VN377_01920"
     CDS             4852..5070
                     /locus_tag="VN377_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HWR27172.1"
                     /translation="MEKPLKLLHASLNNRVIVELRGGVEYQGVLDGYDVPHMNLVLKT
                     SDEIVHGEKKAKHDKIIVRGDNIIYISP"
     gene            5088..>5230
                     /locus_tag="VN377_01925"
     CDS             5088..>5230
                     /locus_tag="VN377_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012608960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HWR27173.1"
                     /translation="MTKGTTCRAGNKTTHIACRRCGRHSFHAQKRRCAFCGFGATSKM
                     RHY"
BASE COUNT         1647 a         1131 c         1036 g         1416 t
ORIGIN      
        1 agcaaaacaa tcaaacaaaa aaggaaaata aaaaaaaagt ggataaaatc tgttttagaa
       61 ttttccgtgt aacttacggc gttccacttt gacaccgttt ggcgaaacga ccatgaggtt
      121 gttaccgatc ccagcaacgt caccaccgat atcatcggtg atcctcttca gctcattggt
      181 gactcgtttc agcagtactt cctgatcgga gatcgttgtg aaatcaagga tgagcacgtt
      241 tccaccgtag acgaaatccg ttaaatcctt taaatcctca taccgttgaa tatcaccaat
      301 ggcaacacgc atgcgtgcac cggtcatacc tccgacttgt gcatccgcat atttctcaag
      361 atcgatatat ccctctgttg ttgatgtctt tttctcacca aaaatcttac ttgttattcc
      421 cattctgaca cactactcct aatttttgcc agtattctaa aatatatttc gctttaaaaa
      481 gatttgggtt gaaatatatt ttaacgtttt attaaaaaac gacacttaat gcatataacc
      541 tgattttaat gttaaatatc tgggagagta cgaagatcag aatgcagtta tcgacatata
      601 gattttttaa aaagaacttt cttgtttgag gaagatagtt aaggagaaaa attcactagt
      661 catccatcat taatataatc ttttcaaaat gtttaaatga atcaatagga taacaatttt
      721 tatataatac attgttttgg ggatattaga ggtggcatga aaaaaaaggt aattgatctg
      781 tttgtttgta gtctattgat tgcaactatt ccaacagttg gagctttgaa tgggggtggt
      841 ttacaaccaa caactcgaag ttcgtatcct ttggaattgg ctcattggaa tcaaatacag
      901 cagcttcttg tttcggacga catagcaagc gatttagttg ttgatacttc tcttcttaga
      961 gatattacct tgtacgatga ttattccgtt aataatttga aatggccggg ttacgaaact
     1021 ttaggggatc gtgataatca atcaccagtt tttgggacgc catcaccgga taatgattca
     1081 cataatattt cacttagttt tacatggagt atttcgatta acgatactga aggtgattcc
     1141 ttcacttgga ctatcgaatg tgacaatgga caaaaaaatg aagaaaaagg agatgttaat
     1201 ggtacaaaat cacttgagat ttctaatctt ttatacaaga catcatacac ggtctgggtg
     1261 aatgcaactg atgaagatgg gagtggtaat tatactaaga aatggtacat ttttaacaca
     1321 aaggatactc tacctccggt ttttggaaat ccagatccag aaaacggttc gactaataat
     1381 cctttaagtt tcatctggag tattccgatt aacgattctg agggtgacga gttctcctgg
     1441 tcgattcaat gtagcaatgg ggacgtcagt agcgggagta atgagttgaa tggaacgaaa
     1501 atccttgtgc tctccagtct taccttttct acaacataca cagtctgggt gaatgcaact
     1561 gatccagatg ggagtggtgt gttcacaaga agatggtata ccttgactac aaaagataat
     1621 caaccaccta ttttcggatt acctaatccc tcaaatgaat ccataaataa tcctataaac
     1681 ttcatttgga atatctcgat tattgattct gaaggaaata ctttcaattg gacaatccaa
     1741 tgtaacaatg gacaatttaa tagtgggacc gatgaggaca atggaaccaa gttactcgca
     1801 ctttccaatc ttgcatattc tacaacctac attatatggg taaatgcgac tgatgcgacg
     1861 gggagtggtc tgtggaatcg gggttggtat acattcacta ctgaacagga aaatcattct
     1921 cctgattttg gggcaccttc accaactaat ggctcagcta acaacccaat taatctcagt
     1981 tggagtatcc taattagtga ttctgagggt gatacgttta catggaccat ccaatgtaac
     2041 aatggacagt ctaacaatag cgtggttggt gaagccaatg gaacaaagac acttatactc
     2101 tctaacctcg ccaatgcaac aacttatacc gtttgggtga atgccactga ttcaggtggt
     2161 agtggagtct atactcgaag atggtacacc ttcaccacga aaagcaatca acctcctatt
     2221 tttgggtcag ctaccccctc aaatggttcc agcgatagac ctttgagttt cacctggatg
     2281 atcccaatta atgaccttga cggaaattcc tacgattgga cgattcaatg cagcaacgga
     2341 caaaccagta gcgggatgac tgcaaacaat ggtactaaaa cccttgcact ctctggtctt
     2401 gcatacgaga cgacttacag agtctgggtg aatgcgaccg acccaggtgg cagtggtctg
     2461 tacacaagac gatggtacac ctttagaact cgtaatgttg gaggaacccc accagaagaa
     2521 ccgccggaat catcaaataa taaccctatt gcagatgcgt ctgctcggga gccttatcag
     2581 ggattaataa atacaacaat tctctttgat ggatcactca gttatgatcc ggacgggaac
     2641 ataactactt ggttatggga tttcgatgat aacaccaaag tgaatggaat gacagtgtat
     2701 cattccttct cagaagcagg gatctattct gtgaccctta ctgtgaccga taacgacgga
     2761 gcgtcaaaca cagatacaac aacctgtgtt atctcagaac acaatcagcc tccgacaata
     2821 ccaaagatag atggaccgac cagtggtacg aagaataccc tgtataccta tactgcttca
     2881 tcgacagatc cagataatga tcgattgcaa tatagttttt tatgggaggg ttcgatatct
     2941 caaccaagtg ggttcattcc aagtggcata aattattcag tgaatcatag atggatgaat
     3001 gcaggacgct ataatcttac tgtgacagtg accgataacc aaactgaatc ttcatcaaag
     3061 atcactattt acattgatgc cgtacaaaca agaggcgcgg ggtatctgct tgatttggac
     3121 ggtgacggca tctacgatgc gttctattca gatgagaccc accaaacagt gtcaatccaa
     3181 agaaacggtg acagttttct cattgataaa gatggggatg gacattggga gtatgtgtat
     3241 aatgcgacct atggattgac gtcgtaccaa gaaccacgta aaacacctgg ctatgaactc
     3301 ggttttaccc tctgtgcaat tgctgtcgta attctcctta ctcgaaaaag aaaaaccata
     3361 taaacgataa ctacggatac cggtttgggt ttgaggttga acgagaacgg aagacttgtt
     3421 gaacgcataa cactaaaaat caaaatgttc gataccggca aatgggaatg gaccgtccgc
     3481 caacgattca tcaaggaacc acgaatcaaa ctccacgaca tgttaaccat attagatgag
     3541 gtcaaagcag aaatcatcgc aagcgaggaa accgaaaaac aagacgaaat gacacaagaa
     3601 gatgcaattc cagcaacaca acccgaaaca ccaaagacat catgatgacc tgatccagac
     3661 atttttttcc tataaacacc tataatgaac taagagaact caagggatac gtatgctgta
     3721 ttacattcat gggtatcaat caagtcccag cggggaaaaa gcggcactac tcaaagcaac
     3781 attacatgca ataccaatag cttatcgaga gagcgttcct gaggaactcg tcatttcaga
     3841 atgtctcagc cgtatctcag aagctatcgc aaacgatcaa caggtgatac tcattggttc
     3901 ttctcttgga ggattcctgg ctgcatccac cgcactgaca cactctacag tcaaacaact
     3961 catccttctc aacccagcga tcatcccatc agaactcgat ctcaacacca tcgaagggat
     4021 gcctttaaga attctacagg aaatgagaga cccccgactg ttccaacaga agatcaaagc
     4081 aacaataacg atcctgcgag gaacccttga cgatatcgtt ccagatgaat ggatccttac
     4141 tttcgcacag gcacagaatg caaccatcag actgtacacc gatgaccatc ggttctcaaa
     4201 aaacatgagc agactacctg aaatcatctc agagctgata caacagtagc catgtgcaat
     4261 cctaatgcgt caatgtccag aatcgatcgc cgacatactg caaggtttta actgcttttc
     4321 ctggcttttt cgtcttcatc tcctccccac tcatcaaagc aatccctgtc gctaacggtt
     4381 tgcgatgttt ctcatcgcag atccagacaa aatcatcctt ctgaatcatc acatccacat
     4441 cagagatccc cggagccatg acatctgctc ctttcgtaat ataactaatg gcccccatat
     4501 cgatgacgac gtattttgct ttaggctgat gtttcatcaa cccctggagc gtaaacaaaa
     4561 acttgttatt gtgcatcata aaatccatgc tatcatccac cagcaggact gtgaattcct
     4621 caacagatcc gatatcgact cctacttttc ccttaaggaa atcatcaccg aatcgggtct
     4681 gtaaatcagc ggataactcc tttacatccc gttccttcag acgatgccga ttctttatct
     4741 tgacaaccat acagaagata ttccaacgaa tgttttaata gttatcctgg agaagaacgg
     4801 cccaaactgt tataaatgct atctccattt cgtgtctcct aggtaacagc tatggaaaaa
     4861 ccattaaaac tcctgcatgc aagtctgaac aacagagtca tcgtcgagct tcgaggcggt
     4921 gtcgaatacc aaggtgtcct tgatggttat gatgttcctc atatgaacct tgtcttaaaa
     4981 acctctgatg agatcgttca tggcgagaag aaggcaaaac atgacaaaat catcgttaga
     5041 ggcgacaata tcatttatat atcaccttag aataaggggt agctactatg acgaaaggga
     5101 ccacgtgcag agctggtaat aaaaccaccc acattgcatg tcggagatgc ggaagacatt
     5161 ccttccatgc gcaaaagaga cgctgtgcgt tctgtggttt tggggcaacc agtaagatgc
     5221 ggcattataa
//
LOCUS       DATMXJ010000065         7363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53599, whole genome shotgun sequence.
ACCESSION   DATMXJ010000065 DATMXJ010000000
VERSION     DATMXJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53599"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VN377_01930"
     CDS             complement(<1..564)
                     /locus_tag="VN377_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWR27174.1"
                     /translation="MDQCNEVTVIGAGPAGIAAAIYLKRAGLHPILLEAHEPGGLLRY
                     AYLVENYPGFPRGISGKKLAESIVEQLHNLGLSIRKSAVKHVHHNNGVFLIETDQGRL
                     LSPAIIIATGTCPKKVNMMGSASIEGTRLFYDPSSIHLEEDKGKKRIIVIGGGDIAFD
                     YTLTLLHGGHEVTIISRSEPTCLPLLHN"
     gene            complement(543..1178)
                     /locus_tag="VN377_01935"
     CDS             complement(543..1178)
                     /locus_tag="VN377_01935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWR27175.1"
                     /translation="MSIPRDGKRTEKVEHPQYRKIVFKSQTSALLVIDMQQYFCNSSS
                     HAYFKDSTTIIPNIHQLITTYRQLSLPVLFTRYALHRTESPGAMGRWWNDVLYDDNKM
                     SFIIDALHPLPQESVIRKTQYSAFFETDLDKILKNHQVTTIVITGVLTHLCCETTARD
                     AFMRNYDIFFVSDATASDTKTLHSASLATLSDGFATISTTNEVCAWINAMK"
     gene            complement(1396..1605)
                     /locus_tag="VN377_01940"
     CDS             complement(1396..1605)
                     /locus_tag="VN377_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27176.1"
                     /translation="MGKKYIKSLTLDNTLKVTVTAESYNWNWTARTFGKHTVKVVAYG
                     LENETASKEIQVTKFLQSFDIFSSN"
     gene            complement(1746..2309)
                     /locus_tag="VN377_01945"
     CDS             complement(1746..2309)
                     /locus_tag="VN377_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27177.1"
                     /translation="MKQNKYAQALATAFVFIFLMVCFSGCTSNQNVNGNGNIFSGTWV
                     GNVEMSLFGRSGNTSVSKIAFSGNTVELTVNTEQGTFTTNYTYSINGSTFILEPQFNG
                     RGGFPGRQPYNGTHSGNWTRPSANETWPINGTMPDNSTQSSNWTRPGNGTWNPGNERP
                     SMSISFTFSVNEDHTVIYLNGAEFRKI"
     gene            complement(2313..3611)
                     /locus_tag="VN377_01950"
     CDS             complement(2313..3611)
                     /locus_tag="VN377_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27178.1"
                     /translation="MKLADILKLSLNSLTHRGLRSWLTILGIIIGVAAVIAMLSIGAG
                     MSQNMKTQLSSFGTDVITVSAGRTRAQGPEGGFGDRFQPGGGFSQTTGTTSSTTTTKL
                     TDKDINAILTAQGVDTVSGIITGRASVQYLAQTVTVTVEGINPTAWNSMTTSKLELGR
                     FLEQGDGTSVLIGYNVAHEMFNSNLTENSPIKIGGKTFSVAGILQQSGTGGFGGDDRT
                     IFMTLDTARDIVTGLDSDQYSSLQIKITNTNAVDQIIQNVDQVLYTSRMVTSDTADFT
                     VTSPTAMLQTIQSTMATLTFFLTGIAAISLLVGAIGIANTMFMSVMERTRLIGILKSI
                     GTRNSEIMKLFLTESGIIGLMGGLLGVFLGLIVVGIISSIGINIMGMGRLGTSTSVAI
                     VTPELVLFALLFSTIIGIISGLIPARKAANLQVVEAMRSE"
     gene            complement(3608..4981)
                     /locus_tag="VN377_01955"
     CDS             complement(3608..4981)
                     /locus_tag="VN377_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27179.1"
                     /translation="MKKINYMIISVLSCVLLFNTLGSFNASAAGLQVSFSTNPKIVAP
                     GTNGYIEVNLRSTSTVISSIDITAESWDPAVVIPQGNWEVDVGSLDSGESYTVIFEFK
                     IPSTAAPGLYQVLFEITGGNTIRQTAVIQVQDATILDLTSVTPPEITVGVATTLLFNI
                     TNNGGVGLENILFTWKDPNSLILPLGADNRITIPSIGAENHTEIPVVVMASSGISPGI
                     YPLTITMEYYDQTGMKQMVNSTVGLQVSGTTTFDLVVQQSTSGSTTFSVVNTGANVAS
                     SVIVSIPQQPNYRTSGVSSTSLGNLNAGDYTLATFQLSSTTNNSTSQFPSFNRTETGT
                     QTGRNFSGRNTFMNQSFAGLRGNSLLIQITYTDVFGIRQTIQKQVTMSSSSSFGSIGS
                     PTSRSSTGTQNSFGGFSQSQSSGSNNSLVYIAIGAAGIIVIIGIIQLGRKKKLTRITK
                     IFKGRKE"
     gene            complement(4986..5669)
                     /locus_tag="VN377_01960"
     CDS             complement(4986..5669)
                     /locus_tag="VN377_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27180.1"
                     /translation="MTTAIIELQDVKKIYKMNGVETPALRGVNLKINKGEFVAIMGPS
                     GSGKSTLLHMVGALDTPTSGKVFLDGVDISTLKESDLARLRGEKIGIVFQFFNLHPTL
                     TALENIELPMIIIEKDKKERQQRALELIKAVGLEHRAHHLPSQMSGGEQQRIAIARAL
                     ANNPEIILADELTGNLDTKTKAEIMSFLLQLQKEKKMTVTVITHEPEIAQYAETIIHI
                     VDGKIKIGG"
     gene            complement(6121..7335)
                     /locus_tag="VN377_01965"
     CDS             complement(6121..7335)
                     /locus_tag="VN377_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWR27181.1"
                     /translation="MNESFETKKKSKNKNILMVVAIGVATMFIAGYFVGGSLNSLATS
                     STTESQKQQGNILNNNTISEEVHNITYYYNTTSLAQLYNDVKNSVVVITGYVIQYSFF
                     GRQYSEVQGSGFIYEYDNDMVVITNKHVVNEAENITVTFSNGDGYPSTVIGSDAYSDL
                     AVLSVDAPSEEFHPLEITSSSSLQVGDPVVAIGSPFGLGGTMTTGIVSQLGRTIQDSV
                     AGNFPIANIIQTSAAINPGNSGGPLLNYEGEVIGITTAIIQNSNGLGFAVPSNTILRE
                     LESLITTGTYDQHPWVGISGTDMTYAIAQILEVDVTYGWLIAQVTDGGAAAQAGLLGG
                     NEQVRVNDAWVMIGGDIIIAVDGTRIINGDTFMSYLEEHTTPNQTITMTILRNNQIQD
                     VSVVLKQRPSTN"
BASE COUNT         1891 a         1589 c         1548 g         2335 t
ORIGIN      
        1 gttgtgaagg agtggtaagc aggttggttc tgaacgagaa atgatcgtca cctcatgccc
       61 cccatgcaac aaggtcaatg tgtaatcaaa tgcgatgtct cctccgccga taacaatgat
      121 tctttttttt cccttatctt cttcaagatg tattgaggaa ggatcataaa acagccgtgt
      181 gccttcgatg gacgcggaac ccatcatatt tacctttttt gggcaggtcc ccgtagctat
      241 gataatcgca ggtgaaagta aacgcccttg atctgtttca atgagaaaca ctccattatt
      301 atgatgaaca tgttttaccg ctgattttct gattgataga cccagattgt gaagttgctc
      361 aacaatcgat tctgctagtt tttttccact gataccacgg ggaaaaccag gataattctc
      421 aaccagatac gcgtatctga ggaggcctcc tggctcatga gcctccagaa gaattgggtg
      481 taaccctgct cgtttgaggt aaatcgctgc agcaatacct gcgggaccag caccaatgac
      541 cgttacttca ttgcattgat ccatgcacag acctcgtttg tggtcgatat ggtggcaaaa
      601 ccatcagaaa gtgttgcaag acttgcggaa tgtaacgtct ttgtatcaga tgctgtcgca
      661 tcacttacga aaaatatgtc ataatttctc atgaacgcat ctcgtgctgt tgtttcgcaa
      721 cagagatggg ttaacacccc ggtgattaca atggtcgtaa cctgatgatt tttgagaatc
      781 ttgtcaagat ctgtttcaaa gaaggcgctg tattgtgttt ttcggattac agattcttgt
      841 ggcagcggat gaagtgcatc gatgataaat gacatcttat tatcatcata caagacgtcg
      901 ttccaccaac ggcccatcgc tccagggctt tctgttcgat gcaacgcata tcgggtgaag
      961 agaactggaa gggataattg tcgataggtg gtgatgagct ggtgtatatt tggtatgatt
     1021 gttgtcgaat ccttgaagta tgcatgagac gaggaattgc agaaatactg ttgcatatcg
     1081 atgacaagca atgccgatgt ctgggattta aaaacgattt ttctatattg aggatgttct
     1141 actttttcag tacgtttacc atcgcgtggt attgacattt ctttcccaca tcagtacatt
     1201 ttattgtttc ctggtttgtg aggctggaaa cataaaaaaa caatgcctcc ttcgaaataa
     1261 agaaatacaa taagtggtaa aaaaagttaa tgctttagtc acccccgaca ttgagatctt
     1321 tggaatatca ttttcataat atatttttac cggtaaaatg ggctgtcaaa tctcctcctc
     1381 agcgcttttt tttacctagt tggaggaaaa aatatcaaat gattgcagga atttcgtcac
     1441 ctggatttcc ttgcttgcag tttcattttc caaaccgtat gcgacaacct taacggtatg
     1501 ttttccaaat gttcttgcag tccaattcca gttgtaggac tcagcggtca ccgttacttt
     1561 taaggtattg tcgagggtaa gactctttat atattttttt cccaacttat acccgaatag
     1621 tgcgatgaga tgtttgttat agtatcggaa aattatgttg aggttttttc tcgtgcattt
     1681 ttttctagac gagacaaatg gattttcacc agaaatttag ttattggttt tcatcccaaa
     1741 cgaacttata tttttctaaa ttcagcaccg ttgaggtata taacagtatg atcttcattg
     1801 acgctaaaag tgaaagatat tgacatcgat ggtcgttcat tccctggatt ccatgtcccg
     1861 tttccgggtc ttgtccaatt cgacgattgt gtactattat ccggcattgt cccatttatc
     1921 ggccatgttt catttgcgga tggtctggtc caattccctg aatgggtacc attatatggt
     1981 tgtcttccag gaaatccgcc tctcccatta aattgtggtt ctaaaataaa cgtcgatccg
     2041 tttatagaat acgtataatt cgttgtaaat gttccttgtt ctgtatttac cgtcaattcc
     2101 acggtattac cagaaaacgc tatcttagaa accgaggtat tcccacttct accaaacaac
     2161 gacatctcga cattcccaac ccatgttcca ctaaagatat ttccatttcc gtttacattt
     2221 tgatttgatg tacatccact gaaacaaacc atcaagaaaa taaatacaaa tgcagtagcc
     2281 agcgcctgcg catacttatt ttgcttcata cattattcac ttctcattgc ttctacaacc
     2341 tgtagattgg cagcttttcg agctgggatc aaccctgaga tgattccaat aatggtcgaa
     2401 aacaataacg cgaacagaac gagttcgggg gtcactatgg caacagaggt gcttgtaccg
     2461 agtctgccca tgcccatgat gttgattccg atactcgaga taataccaac aacgatgagc
     2521 ccaaggaaca ccccgaggag tccacccata agaccaataa tccctgattc agtaaggaat
     2581 agtttcatga tttcagagtt tcgtgttcca atggatttca gaataccgat aagcctggtt
     2641 cgctccatca cagacataaa catggtattt gctatcccga ttgctcctac cagaagggag
     2701 atagcagcaa ttccggtcag gaaaaacgtt aacgtcgcca tggtactttg aatggtttga
     2761 agcatcgctg ttggtgacgt caccgtgaaa tccgcggtgt cagaagtgac cattcgcgaa
     2821 gtatagagta cttggtcaac gttttgtata atttgatcaa cagcattcgt atttgttatt
     2881 tttatttgca gtgaggaata ttgatcagag tccagcccgg taacaatatc tcgtgcagta
     2941 tcaagtgtca tgaatattgt tcgatcgtct cccccgaatc ccccggtacc ggattgttgg
     3001 aggatcccgg cgacgctgaa tgtttttccc ccgattttta tcggactgtt ctctgtaaga
     3061 ttcgagttaa acatctcatg ggcgacgtta tatccgataa gaacgcttgt cccgtctccc
     3121 tgctcgagga atcttccaag ttccagtttc gatgtcgtca tcgaattcca agcagtgggg
     3181 ttgatgcctt cgacggtcac cgtcacggtc tgtgccaggt attgtaccga tgcccgaccg
     3241 gtgatgatcc ctgagactgt atcgacaccc tgcgcggtga gtatcgcatt gatatcttta
     3301 tccgtcaatt tcgttgtcgt cgtgcttgat gttgttccag ttgtttgaga gaaaccacca
     3361 cccggctgga atctatcccc gaaacctccc tctggtcctt gtgcccgcgt ccgccctgca
     3421 ctcaccgtaa tgacatcggt gccaaagctt gacagctgtg tcttcatgtt ttgagacatc
     3481 cccgcaccaa tcgatagcat ggcgatcaca gcagctaccc cgatgataat tccgaggatg
     3541 gttagccagc tgcgtaaccc gcgatgggtc aaactattta atgacagttt gaggatatca
     3601 gccagtttca ttcttttcgt cctttaaaga tttttgtgat acgtgtcagt ttcttctttc
     3661 gtcctaattg tatgatgcca ataatgacga tgatgccagc ggctcctatg gcgatgtaca
     3721 caagactatt gttagaacca gatgattgcg attgactgaa tcccccaaaa ctgttttgtg
     3781 ttcctgtact gcttcgagag gtagggcttc caatggatcc gaaagaggaa ctggaagaca
     3841 tggtcacctg tttttgaatc gtctgtcgta tgccaaatac atcggtatag gttatttgaa
     3901 taaggagaga attccccctt aatcctgcaa aggattggtt cataaacgtg tttcggccgc
     3961 taaaattcct tcctgtttgt gtaccagttt ccgttctgtt aaaagaggga aactgagatg
     4021 tcgaattatt cgtagttgaa gaaagttgaa acgtcgctaa ggtgtaatca ccggcattaa
     4081 ggttccctag acttgttgaa gacacaccgc tcgtccgata atttggttgt tgaggaatcg
     4141 atacaataac cgacgatgca acattggctc ctgtattcac aacggaaaat gttgttgatc
     4201 cgctggtcga ttgttgtacc acaagatcaa aagttgtcgt accactgact tgcaaaccaa
     4261 ctgttgaatt caccatttgc ttcattccag tctgatcata atattccatg gttatagtaa
     4321 gtgggtaaat acccggggaa ataccggagc ttgccatgac aacaacggga atctctgtat
     4381 ggttttcagc accaattgag ggaattgtaa ttctattgtc cgccccaaga gggagaataa
     4441 ggctatttgg atctttccag gtaaagagga tattttctaa tcctactcca ccgttgtttg
     4501 tgatattaaa tagaagggtt gttgctactc cgacggttat ctccgggggt gtaaccgatg
     4561 ttaaatccaa aattgtcgca tcctgtactt gaatgaccgc ggtttgtctg attgtgtttc
     4621 ctccagtaat ttcaaaaagc acttggtaca accctggagc ggcggtgctt ggtattttga
     4681 attcgaagat gacagtatag gattcaccac tgtctaaact tccgacatcg acttcccagt
     4741 ttccctgtgg gatgacgaca gctggatccc acgattctgc tgtaatatca atgctgctga
     4801 tcactgtgct ggtgcttcta agattcacct cgatgtaccc gtttgtccct ggtgcgacaa
     4861 tctttgggtt tgtcgaaaaa ctcacttgaa gccctgctgc agaggcgttg aaactgccta
     4921 acgtattgaa gaggagtacg caactcaata cacttattat catatagttt attttcttca
     4981 tttttctaac caccaatctt tatttttcca tctacaatat gaatgattgt ttctgcatat
     5041 tgtgctattt ccggttcatg tgttatcaca gtgaccgtca tttttttctc cttctgtaac
     5101 tgtaataaaa aactcatgat ttccgcttta gtcttcgtat ccaaattccc cgtcagttca
     5161 tctgctagaa tgatctcagg attgttcgct aacgctcgcg caatcgcgat acgttgctgt
     5221 tcaccaccag acatctggga tggaagatga tgtgcccgat gctccaaccc aaccgctttt
     5281 atcagctcaa gggctctctg ttgtcgttct tttttatctt tttcaataat gatcatcgga
     5341 agctcgatat tctccagcgc tgtcaaggtg ggatgcaggt tgaaaaattg gaagacaata
     5401 cctatttttt cgcctcggag acgagcaaga tctgattctt ttaatgttga aatatcgaca
     5461 ccatcgagaa atactttccc tgatgttggt gtgtccagtg caccgaccat atgtaacaac
     5521 gttgattttc ctgagccact tggtcccatg attgcgacaa attcaccttt gttgattttt
     5581 agattcacac cacggagtgc gggagtttcg acaccattca ttttgtaaat ttttttcacg
     5641 tcttgaagct cgatgattgc cgtcgtcatt ttttttcacc tttttgttgt cgatttggtt
     5701 ttatttgttc agtatgattt cctgatttgt tgttttttgt taccctggat tcaccttcaa
     5761 aactatatat cgatttttcc caattcattc ccaatttcat tccgaattgg aaaaaagatc
     5821 tacttctcct gacgaaaaat aatttgagta aacctttcaa taaattttct actcaatgtg
     5881 acgttctttg tgtccgtttc atttgatttc aacgaatgaa gatttacttc tatgttgtct
     5941 cgggttgttc tttttttact catataattt catttctttt tctctctctc gattcgtgat
     6001 attttttatt gaattgtgct tgatacattg ataaaaaaga aagagggatg agtcgtgtca
     6061 agatagaaac acaaagatgg aggagagcaa tagggaaagt tgacacaact catcggttgg
     6121 ttaatttgta cttggtcgct gtttcagtac cacggagaca tcctggatct gattgttccg
     6181 gagaatcgtc atcgtgatgg tttggtttgg agtcgtgtgt tcttcgagat aggacatgaa
     6241 ggtatcaccg ttgatgatgc gtgttccatc cactgcgatg atgatgtcac caccgatcat
     6301 cacccatgca tcgttgactc tcacctgctc gtttccaccg agtaaccctg cctgggcagc
     6361 agctccgccg tctgtcactt gtgcgataag ccatccgtag gtaacatcta cttccaagat
     6421 ctgtgcgatt gcataagtca tatcagtccc tgatattcct acccacgggt gttgatcgta
     6481 ggttcctgtc gtaataagtg attctaattc ccgtagtatg gtgttcgatg gaactgcaaa
     6541 accaagtccg tttgaattct gtataatcgc cgtggtaatc cctatgactt ctccttcgta
     6601 attcaaaaga ggtcctcctg agtttcctgg gttgatcgca gcgctggtct gaatgatgtt
     6661 tgcgattggg aaattccctg cgacagaatc ctgaatagtt ctcccaagtt ggctgacaat
     6721 tccagtggtc attgttccac ctaatccgaa gggactgccg atagcgacaa caggatcacc
     6781 gacttgtaag gacgaggaac tcgtgatttc caaaggatgg aactcttcag agggagcatc
     6841 gacagataag actgctaaat cagagtatgc atccgagccg atgaccgtgg aaggatatcc
     6901 atccccgttt gagaaagtca ccgtgatgtt ttctgcttca ttgacaacgt gcttattggt
     6961 gatgactacc atatcattgt catattcata gataaaacca gagccttgga cttcagaata
     7021 ttgcctgccg aaaaatgagt attgaatgac gtatcctgtg atgacgacaa ctgagttctt
     7081 cacatcattg tataattgcg ctagggacgt cgtgttataa taataggtga tattgtgtac
     7141 ctcctcagaa atcgtattat tattcaaaat atttccttgc tgtttttggg attcggttgt
     7201 tgatgatgtt gcaagcgagt tgagcgatcc accaacgaaa taccctgcga taaacatggt
     7261 ggctacgcca attgcaacaa ccatgaggat gtttttgttt tttgatttct tttttgtttc
     7321 aaaactctcg ttcattcaca atacctccgt ctttttttct ttt
//
LOCUS       DATMXJ010000066         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53648, whole genome shotgun sequence.
ACCESSION   DATMXJ010000066 DATMXJ010000000
VERSION     DATMXJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53648"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VN377_01970"
     CDS             <1..594
                     /locus_tag="VN377_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWR27182.1"
                     /translation="VMAAAPILALVVQALDKAYHIGSTNLPAPQAFLMKGIVQGVLGG
                     EMVWPFVVAGAVLAFVLILINLPVLPVAIGIYLPFTLSTPIFAGGIVRAITNRAVAKK
                     YGTAQEEEISDWDLAIKQTDVAPKEKIIRTGLLLTAGLIAGEALMGVIVAFLIIGGLN
                     FAVFEYPPILPSVLIWVFIAALLAYIPLREIYAKDEK"
     gene            620..931
                     /locus_tag="VN377_01975"
     CDS             620..931
                     /locus_tag="VN377_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HWR27183.1"
                     /translation="MVYKPKRLDFKWSADTNGLVTVTVPKFTGKLGKSFCKVLKRQNT
                     FEAHLDRLGSSIWNQCDGTTFVKDILIVITKEFPDEKNIDQRLFLFLQQMKALNYITY
                     Y"
     gene            complement(925..1596)
                     /locus_tag="VN377_01980"
     CDS             complement(925..1596)
                     /locus_tag="VN377_01980"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HWR27184.1"
                     /translation="MRVAALFSGGKDSIFAVYITQQYGWEVSHLVSLLPKKPDSWMFH
                     SINIHLTDLQAKSIGIPLVKRATAAEKEAELEDLTQAVQDLEIDGVVSGAIASEYQRT
                     RIEKVCDNLGLKSFSPLWHKNQELLLRDQVKAGFHIIIVGVFAEGFDETWLGRTLDEE
                     TIDALVSLQKKQGINIAGEGGEYETLVLSGPMFSKRLIIDESGKEWNRDSGVFQVKQA
                     HLEGQ"
     gene            1650..2414
                     /locus_tag="VN377_01985"
     CDS             1650..2414
                     /locus_tag="VN377_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HWR27185.1"
                     /translation="MRIGILCGHHMPDLMKNPEKINVHTPYGDIILNVSTVSGHDVFF
                     VHRHGEQENLPPHRVNHLGNIHALAVSHVSCVISVGTVGSMQKTIRPGDFVIPHDFID
                     TTKSRPVTFFDDRRIHVDMTEPFCPSLRKLLLKNCKRLKDVTVHEKGVYLVTEGPRLE
                     TAAEIQLYSTVADIVGMTLAPEVILAREKGMCFVSLCLVCNMAAGLQQRLPVDEITAL
                     YKKKEPLISKILQLTIQSLDEKQPCLCPSDVSQATL"
     gene            2411..2821
                     /locus_tag="VN377_01990"
     CDS             2411..2821
                     /locus_tag="VN377_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWR27186.1"
                     /translation="MKQYELIEHTADVGVKAYGKTIAEAFEHAAKGMFDIITDESTID
                     PVGQYDFQLEAPDLEQLLVDWLSQLIFLNDAQDLVFGKFQVTLTGNRLSASVFGEKYD
                     TKKHRMGVEIKAVTYHMLQVNEKDPIFVQVLFDI"
     gene            3128..3337
                     /locus_tag="VN377_01995"
     CDS             3128..3337
                     /locus_tag="VN377_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HWR27187.1"
                     /translation="MSKEKISEDMTIKEVIDNYPETAMVFMKYNVGCIGCLAASFEKV
                     KDIATVHGIDIKALVKDLNLAVQKK"
     gene            complement(3374..4132)
                     /locus_tag="VN377_02000"
     CDS             complement(3374..4132)
                     /locus_tag="VN377_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HWR27188.1"
                     /translation="MKSAYFDCFSGIAGDMILGALIDLGVDETVLKKELKKLRLTGYD
                     IIVKKVESNHITATDVTVIVTEDHHHRSLSDINDILNRSLLEPQVKKLSQTIFYNLGK
                     AEARIHNVDIEEVHFHEVGAVDSIVDIVGSVIGITLLGIDTIICSPLPLGHGFVSCEH
                     GLLPLPAPATVELLKDIPVYTVERNQETVTPTGAAIITTLASSFGEMPSMKITHIGYG
                     SGKIPSKYPSLLRVFLGELEHKSRKKGTGKTGKK"
     gene            complement(4139..4864)
                     /gene="larB"
                     /locus_tag="VN377_02005"
     CDS             complement(4139..4864)
                     /gene="larB"
                     /locus_tag="VN377_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWR27189.1"
                     /translation="MKKLLQDYKAGKVSLSAVLQKIKTLPYEDLEFAKLDTHREVRKG
                     FPETIFCQGKTIPQISQILQKLPKDQNILLTKANKNVYTSVKKLYPLALYNESAKTIV
                     IQRFKPKKKKGTILILTAGTSDIPVAEEAVTTAELMGNKVEKVYDVGVAGVHRLFDIK
                     EKIFHANVIIVIAGMEGALASIVGGLASKPVIAVPTSVGYGASFQGIAPLLTMLNCCA
                     EGVVVVNIDNGFGAGYFASLINR"
BASE COUNT         1312 a         1174 c         1156 g         1397 t
ORIGIN      
        1 gttatggcag cagccccgat tcttgcgttg gtcgttcaag cgttggataa agcatatcat
       61 atcggcagta cgaatttgcc agcaccgcag gcgttcctca tgaaggggat cgtccagggt
      121 gtccttggcg gcgaaatggt ctggccattc gtggtcgctg gagcagtcct tgcgtttgta
      181 ttaatcctca ttaacctgcc tgtgcttcct gttgcaatcg gtatttatct gccgttcaca
      241 ctcagcaccc ctatcttcgc gggtggtatc gtccgtgcca ttacgaaccg tgcggttgcc
      301 aagaaatacg ggaccgcgca ggaagaagag atcagcgact gggaccttgc tatcaaacaa
      361 accgatgttg ctccaaaaga aaagattata agaaccggac ttcttctcac cgcgggattg
      421 atcgcaggag aagccctgat gggtgtcatc gtcgcgttcc tcattattgg aggacttaac
      481 ttcgcagtct tcgaataccc accaatctta ccatcggtgc tcatctgggt cttcatcgca
      541 gcactccttg cttacatccc gttgagggaa atctacgcca aagacgaaaa gtaaaggcct
      601 accgaccgtt gaggagtttt tggtttacaa accaaagcga ttagatttca aatggtcagc
      661 agacaccaat ggtcttgtga ctgtaacagt cccaaaattc actggcaaac tggggaaatc
      721 cttttgtaag gttctgaagc gacagaacac cttcgaagca catcttgata gacttgggtc
      781 ctcgatttgg aaccaatgcg atgggacaac gtttgtcaaa gatatcctca tagtgatcac
      841 caaggaattc cccgatgaaa aaaacattga tcagcgcctc tttttgtttc tgcagcaaat
      901 gaaagctctc aattatatta cgtattattg accctcgaga tgcgcttgtt tcacctggaa
      961 gacgccagag tcacggttcc attcttttcc tgattcatca attatcagtc ttttggaaaa
     1021 cataggcccg gacagcacca gtgtctcata ttctccccct tcaccagcga tgttaattcc
     1081 ctgtttcttc tgtaaggata ccagagcatc aatggtttct tcgtcaaggg ttctcccaag
     1141 ccaggtctca tcaaaccctt cagcgaacac accgacgatg atgatatgaa accctgcttt
     1201 cacctggtcc cgtaacaaca gttcctggtt tttatgccac aaaggcgaaa acgattttaa
     1261 cccaagatta tcacacacct tctcaatgcg ggttcgctga tactccgagg caatcgctcc
     1321 actgaccacc ccgtcgatct caagatcctg caccgcctgt gtaaggtctt ctagctctgc
     1381 ttctttttca gcagcggtcg ctcgtttcac aagtggtata ccgatgcttt ttgcttgcag
     1441 gtcggtaaga tgaatattga tagagtgaaa catccaggag tcaggttttt ttggaagtag
     1501 gctgacgagg tgggagacct cccacccata ttgctgggtg atgtacacag caaagatcga
     1561 atccttccca ccggagaata acgctgcgac tctcatcgga ataggtatct ctttaaacaa
     1621 ataaacattt cttggtggtt ggttggtcaa tgagaattgg gatactctgc gggcatcaca
     1681 tgccggatct gatgaaaaac ccagaaaaaa tcaatgtgca tactccatat ggagatatta
     1741 tcctgaatgt ttcaacagtc agtggccatg acgttttctt tgttcaccgt catggggaac
     1801 aagagaatct tccgcctcat cgggtcaacc atcttgggaa tatccatgcg ttggctgtca
     1861 gtcacgtctc ctgtgtcatc tcggttggga cggtcggatc catgcagaag acgattcggc
     1921 caggtgattt tgtgatccca catgatttta ttgatacgac caaatccaga ccggtcacgt
     1981 tctttgatga ccgaaggatc catgtggaca tgacagagcc attctgccct tcccttcgaa
     2041 agcttctctt aaaaaactgt aagagactca aggatgtcac ggtccatgaa aaaggtgtgt
     2101 atcttgtcac ggaaggacct cggttggaga ccgctgctga gatacagttg tactccaccg
     2161 tcgctgatat cgtcgggatg acgttagcac ctgaggtgat attggctaga gaaaaaggca
     2221 tgtgctttgt ctcactctgt ctggtctgca acatggcagc tggtctgcag cagcgattac
     2281 cagttgatga gatcacagcc ctatacaaga agaaagaacc attgatttca aagatcctcc
     2341 agcttacgat tcagtctctt gatgagaaac aaccctgtct ttgtcccagt gacgtctctc
     2401 aggcaacatt atgaaacagt acgagcttat cgagcatacc gctgacgtcg gcgtgaaagc
     2461 atatgggaag accatcgcag aagccttcga gcatgctgcg aaggggatgt ttgatatcat
     2521 tactgatgag tcgacgattg accctgtcgg ccaatatgat ttccaattgg aagctcctga
     2581 ccttgaacag ctgctggtag attggctctc acaattgatt tttttgaatg atgcacagga
     2641 tcttgtgttt ggaaagttcc aagtaactct aacagggaat cgactctccg cttctgtctt
     2701 tggtgagaag tatgatacca agaagcatcg gatgggtgtt gagatcaaag cagtgacgta
     2761 ccatatgctt caggtaaacg agaaagaccc catctttgta caggtactgt ttgacattta
     2821 aaatagaggg agatacataa agagacctca tgatgatata ccgattgaaa tagtcgtacc
     2881 atcgataagt gaatagaaga acgctgttga ttaatcgttt ttgaatagtt tcatcgacct
     2941 gttgtcttta tttaaatacg tagaagacag tgcaccatgt aataggaagc atctaccgga
     3001 cgggacgcct tcctagttga agaatacttt ttttgccgga tcccaccgtc cggtctcctc
     3061 accatagtct aatacgaatc cttatatggg aggtctttat accgaggaaa tacagaatgg
     3121 tgaaatcatg agtaaagaga agatttctga ggacatgacc attaaggaag tcattgataa
     3181 ttacccagag accgcaatgg tgttcatgaa gtacaatgtc ggttgcatcg gctgtcttgc
     3241 cgcgtctttt gagaaagtca aggatatcgc gacagtacat ggcatcgaca ttaaagcatt
     3301 ggtgaaggat ttaaacctcg cggtccagaa gaaataaaaa atattcagat actaaagagg
     3361 caatcatttc tctttatttt tttcccgttt tccctgtgcc tttcttccgt gatttatgtt
     3421 caagttcccc gaggaataca cgtaaaagac ttggatattt actgggtatt ttcccactcc
     3481 cgtaccctat gtgcgtgatt ttcatcgaag gcatctcacc aaagcttgat gcaagagtcg
     3541 tgatgatcgc tgcaccagtc ggggtgacgg tctcttgatt cctctcaaca gtatacacag
     3601 ggatgtcttt gagaagctcg acggtcgccg gggcaggtaa cggaagcaga ccatgctcac
     3661 aggacacgaa cccatggcca agaggcagag gagaacaaat aatcgtatca atgccaagga
     3721 gggtaattcc gatgacggag ccgacgatgt cgacgatgga atcaaccgca ccaacctcat
     3781 gaaaatgtac ttcttcaata tccacgttat gaatccttgc ttctgctttt ccaaggttat
     3841 aaaagatggt ttgactcagt ttctttacct gaggttctaa taaactgcga ttgaggatat
     3901 cattgatatc tgagagactc cgatgatgat gatcctctgt gactatgacg gtgacatccg
     3961 tcgcagtgat atggttcgac tcaacttttt ttactatgat gtcgtatcca gtgagccgga
     4021 gttttttcaa ctcttttttt aaaacagtct catcaacccc taaatcgatg agggcaccca
     4081 ggatcatgtc ccccgcgatc cctgagaaac aatcaaaata cgcacttttc atgattcttc
     4141 acctgttgat gaggctagcg aaatatcctg caccaaatcc gttatcaatg tttaccacca
     4201 ccacaccttc cgcgcagcag ttcaacatag tcagcaaagg cgcaatgcct tggaagcttg
     4261 cgccgtaccc gacacttgtt gggaccgcga tgactggttt tgaggcaaga ccaccgacaa
     4321 tactggcaag ggcgccttcc atcccggcga tcacgatgat gacgttcgca tgaaagatct
     4381 tctctttgat atcgaacaga cggtggacgc ctgcgacacc aacatcgtag actttctcca
     4441 ccttgtttcc cataagctcc gcggtcgtca ctgcttcctc tgcgacaggg atatcactgg
     4501 ttcctgccgt gagaataagg atagttcctt tctttttttt gggtttgaag cgctggatga
     4561 caatcgtttt tgctgattca ttatagaggg ctaaaggata caatttcttt actgaggtgt
     4621 aaacgttttt gtttgctttt gtcagcagga tgttttggtc ctttgggagt ttttggagga
     4681 tctgggaaat ctgaggaatg gttttcccct ggcagaagat cgtctcagga aatcctttgc
     4741 ggacctcccg atgggtatcg agtttggcga actccaaatc ctcatacggc aaggttttga
     4801 ttttctgcag gacagctgac agacttactt tccctgcctt gtagtcttgc aacagttttt
     4861 tcatgtgtca agcacctcgt ttaaactccc agtccggtat ccttcaaggt ccagcgtaat
     4921 gtaggtaaaa ccgagtgttt taaaacgttt tatgatcttt tgttcatgtg taaggattgt
     4981 ttgaaaatca tccggggaca cctcgattcg tgcgatacgg tcatggtacc ggacacgac
//
LOCUS       DATMXJ010000067         6802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_11758, whole genome shotgun sequence.
ACCESSION   DATMXJ010000067 DATMXJ010000000
VERSION     DATMXJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11758"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VN377_02010"
     CDS             <1..401
                     /locus_tag="VN377_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HWR27190.1"
                     /translation="RDVEMSVQSAATLMSNILNSRKFYPYYVQLILGGWDSSGGHVYS
                     LDAAGGSIPDKYTAGGSGSPYAFGVLEDSFKDDMTISDGIDLALRAITAAMGRDSASG
                     NGADVAVITEKEFKMISEDEIKKRLQKMGK"
     gene            482..2392
                     /locus_tag="VN377_02015"
     CDS             482..2392
                     /locus_tag="VN377_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HWR27191.1"
                     /translation="MTVDDVLADIRGKIRSSIPASVDISEVEFEGALLVIYTKTPDKF
                     ANNKDLVKNMAKSLQKRIVVRPDPSVLTDIDIAEKKIRQIIPKEAEITNIYFQPDVGE
                     VTIEALKPGAAIGREGQLLNEIRKKINWAPSIVRAPPIQSKTVQEIRGYLRSMSDERK
                     DILRKIGRKIHRGSSTGEKFIRMISLGGFREVGRSCSMLHTQDSKVLIDCGIDVSSEN
                     NGSPYIHMPEVLPLEKIDAVVITHAHLDHCGLVPILYKYGYEGPIYCTPPTRDLMTLL
                     QMDYIKVAAADAKKVPYSSENIRNVIKHCIVMGYGDTTDITPDIRLTFHNAGHILGSS
                     ICHFHIGDGLYNIAFSGDIKFERTWLFNPAINHFPRAEALVIESTYGGHDDFQPSRKE
                     ATDRLKDIIRTSMKKKGKVLVPVFAVGRSQEVMIVLESLVKMKEIPDVPVYLDGMIWE
                     ATAIHTAYPEYLNNKLRTQIFQQGDNPLLSEIFKRVDSGDMREKILGEKDPCVVLATS
                     GMMNGGPVMEYFKHWCGEAKNTLVFVGYQAEGTIGRRIQRGAKEIPMNVGGNIVSLPV
                     EMNVETCDGFSGHSDRRQLVGFINNMSPRPERVVFGHGEESKCVDLSSTIHKRLNMNT
                     AAPFNLESIRFV"
     gene            complement(2448..3638)
                     /gene="priS"
                     /locus_tag="VN377_02020"
     CDS             complement(2448..3638)
                     /gene="priS"
                     /locus_tag="VN377_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HWR27192.1"
                     /translation="MGAENSGQKSMEFLKNTFQTYYASNKIELPDRFGRREFAFLLFG
                     GKGMIRHLGFEKREMIWNFLTDRAPQHAYYSSAYYQHPDAAKMPDKIWMGAELIFDLD
                     SDHLPNAEKMGYVESLVEVKKEFIKLIHEFLLGDFGFKEKHMELYFSGGRGYHCHVKD
                     PSILDLDSNERREIVDYITGRDLQDSLIFHEQATSRKNYGAYSFAGGKSLKMPTPDQD
                     GWKGRISRGIIDLVNEIKISENPEKKLEEYGVSPHDAERLVQELSEERMRRIKDGLLD
                     QSKTIRKFFLNNALRKTAVSLSAGETDEPVTCDVKRLIRLPSSLHGKTGFKVVKIGVD
                     DLKDFDPLQDAVALPDDPVKIMVQKETTIDMKDQSYHLEEGAQEVPTYLGVFLIGRKA
                     AILL"
     gene            3727..4905
                     /locus_tag="VN377_02025"
     CDS             3727..4905
                     /locus_tag="VN377_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR27193.1"
                     /translation="MGSIIEDELFSSSVIKDLHALDFDYVPSELPHRTEQLKKLAQMF
                     KPVFVDIAQNVLIRGSVGTGKTAMTKYFCQSLVQIARKQGKILEYVHINCRKRSTDAM
                     VLLGILNHFDARFPDRGFSVQEMLQVLRTNLQRKESHLILVLDEVDALVKKGKSNLIY
                     DLTRFNDETTKTGASVSVIMISQKDVFTELDTAALSTFKRSNTIILERYTRDELYDII
                     QQRVRLAFHANTVRAESVDLIADIASEYGDARFSIELLWKAGMYADEKHVKQVVPEHV
                     RVAKAETYSVVTETKLKNLGKHQLLTLLSIAKRLQKEETAYANTGEVEKTYEITCEEY
                     SEDARAHTMFWSYLKEIELAGFIRVKPSGKGQVGNSQLISLPDIPAEVLRSKLEELLT
                     "
     gene            complement(4885..5586)
                     /locus_tag="VN377_02030"
     CDS             complement(4885..5586)
                     /locus_tag="VN377_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HWR27194.1"
                     /translation="MATSPKNLYEILSDTFGHQDWWPVDRKYHQRQHSDPRFEIIIGA
                     ILTQNTAWTNVEKALKNLKEQSIFTIPAMIHVEEKNLKTMIQPSGFFNQKAQRLKFFA
                     AYLEKKYDGDLQRFFSRDTPEIRHELLSLPGIGPETADSILLYAGNHPVFVIDAYTKR
                     ICLRVPLSVLSDSYRDIQQFFENGLSSKIPETERILAFKEFHALLVELAKQYCWKKKP
                     NCSTCPLNHLCEKLL"
     gene            complement(5607..6236)
                     /locus_tag="VN377_02035"
     CDS             complement(5607..6236)
                     /locus_tag="VN377_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HWR27195.1"
                     /translation="MTKLVKQNIKMFLDEVASNSPAPGGGSVAALSGALGAALSSMVC
                     NLTRGKQGYETVQDEIREILGKSEELRKDLTDLIDKDTDAFNEVMKALKMSKETEDQK
                     ELRRNALQTAFKQAAEIPLETARKCVQILDVAHIVAVKGNKNSITDAAVSALMAQTGV
                     QAAMLNVRVNLSSIKDTEYVQQVTTELHELLQNAMQKSAEILGIVEKNL"
     gene            complement(6233..>6802)
                     /gene="ftcD"
                     /locus_tag="VN377_02040"
     CDS             complement(6233..>6802)
                     /gene="ftcD"
                     /locus_tag="VN377_02040"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWR27196.1"
                     /translation="EKTNVPCYLYEEAAKREGRRNLADVRRGEYEGLKAEIGKNPEKV
                     PDYGPNKIHPTAGATAVGARFFLIAFNVNLGTSNIKIADEIAKVVRHSSGGYRYVKAK
                     GFEIKEKGIVQVSMNLTNYQKTSIYRVFETIKNEAERFGVPVIGSEIVGLLPLEALVD
                     STNHYLRLEKFTVDQVLEKRLLEIQKESS"
BASE COUNT         1799 a         1546 c         1523 g         1934 t
ORIGIN      
        1 aaagagacgt agaaatgtca gtgcaaagtg ctgcgacact catgtctaat attctgaact
       61 cccggaagtt ctatccctac tatgtccagt tgatcctcgg tggatgggat tcttcaggag
      121 gacatgtcta ttcattggat gcagctggtg gttctatacc agataaatac accgcaggtg
      181 gatctggttc accctatgca ttcggtgttt tagaggactc gtttaaggat gatatgacca
      241 taagtgacgg tattgatctt gctctcaggg cgattactgc tgcgatgggc cgagattctg
      301 cctcaggaaa tggagcagat gtggcagtga tcactgagaa agaatttaaa atgatctctg
      361 aagatgaaat taagaaacga ttgcagaaga tggggaagta aaaatccgta ttctcaaagg
      421 ataatcgtca tataaacgga gtgttcaaag ttcgttttac aagctttaaa aaaggtagat
      481 tatgaccgtt gatgatgttt tagcagatat acgaggcaag atacgaagct ccataccagc
      541 aagtgttgac atctccgaag tcgaattcga aggcgcactc ctggttattt acacaaagac
      601 accagacaaa tttgccaata ataaagattt agtaaagaac atggcaaaaa gtctacagaa
      661 acgtatcgtg gtgcgtcctg atccttctgt tcttactgat attgatatcg cagagaagaa
      721 aatacgacag attatcccaa aagaagctga gataacaaat atctattttc aacctgatgt
      781 cggcgaggtc accattgagg cgttaaaacc cggtgctgcg atcggccgag aaggtcaact
      841 attaaacgaa atacgaaaaa agatcaactg ggcgcccagt atcgtacgag cgccaccgat
      901 tcaatcaaaa accgtgcagg agatacgcgg gtacctccgt tcgatgagcg atgaacgaaa
      961 ggatattctt cggaagatcg gtcgtaagat ccatcgtgga tcctcaacag gggaaaagtt
     1021 catccgtatg atctcccttg gtggattccg tgaggtcggt cggtcctgtt cgatgttaca
     1081 cacccaggat agcaaagtat tgatcgactg tggaatcgat gtttcatcgg agaacaacgg
     1141 ctcgccttat attcatatgc ctgaggtact ccctttagag aaaattgatg ctgtggtcat
     1201 tacccatgcc catcttgacc attgtgggct tgtcccaatc ttatacaaat atggatacga
     1261 aggtcctatc tattgcactc caccaactag ggacctgatg accctgcttc agatggatta
     1321 tatcaaggtt gcagcagcag atgcaaaaaa ggttccgtac tcctctgaga atattcgcaa
     1381 tgtgattaaa cattgcatcg tcatggggta tggggacacg accgatatca ccccagatat
     1441 ccgactcacg ttccataacg cgggtcatat cctagggtcc tctatctgtc atttccatat
     1501 cggcgatggg ttatacaaca ttgcgttctc cggcgatatt aagtttgaac gcacctggtt
     1561 gttcaaccca gcaatcaacc atttccctcg cgcagaagcc cttgtcatcg aatcaaccta
     1621 cgggggacat gacgacttcc agccaagcag gaaagaggcg acagatcggc tgaaagatat
     1681 catacgcacg tcgatgaaga aaaaagggaa agtccttgtc cctgtgtttg ctgtcgggcg
     1741 aagccaggaa gtcatgattg tcctggaaag cctggtcaaa atgaaagaaa tcccggatgt
     1801 ccccgtgtat cttgatggga tgatttggga ggcgactgct atccataccg cataccctga
     1861 gtatttgaac aacaagcttc gaacacagat attccaacag ggggacaacc ccttgctttc
     1921 tgagattttc aaacgcgtcg atagcggtga tatgcgggaa aagatacttg gggagaaaga
     1981 cccctgcgtg gtgcttgcga ccagtggtat gatgaacggt ggtccggtca tggagtactt
     2041 taaacactgg tgcggcgaag ccaaaaacac gctggtattc gtcgggtatc aagcggaggg
     2101 caccatcggc cggcggattc aacgtggtgc aaaggaaatc ccaatgaacg tcgggggtaa
     2161 catcgtcagt cttcctgtcg agatgaacgt ggagacctgt gatgggttct ccggacatag
     2221 cgaccgtaga cagctcgtag gattcattaa caacatgtca ccacgacctg aacgggtcgt
     2281 gtttgggcat ggtgaagaat caaaatgtgt ggatctgtcc tcgaccatcc ataaaagact
     2341 gaacatgaac accgcagctc ccttcaacct ggagagcatc cgattcgttt aaagcacatc
     2401 tttttttgta ttaattaagg cgtgttgacc tgtaaaaaat aaaagatcta caggaggatt
     2461 gctgcttttc gtcctatgag aaagaccccc agatatgttg gaacctcttg tgctccttct
     2521 tctaaatgat aactctgatc cttcatatcg atggtcgtct ctttttgtac cataatcttc
     2581 acaggatcat caggtaatgc gaccgcatcc tggagaggat caaagtcttt taaatcatcc
     2641 actccgattt ttactacctt aaacccggtc ttcccatgca gtgatgacgg taaacgaatg
     2701 agtcgtttca catcacaggt gaccggctcg tcggtctctc ctgcgctgag agataccgct
     2761 gtttttcgta acgcattatt taaaaaaaac ttccggatgg tcttcgactg atcaagcagc
     2821 ccatctttta ttcgtctcat ccgctcctcg gaaagctcct gaacaagtct ttccgcatca
     2881 tgcggactga ctccatattc ctctaatttt ttctcaggat tctcacttat tttgatttca
     2941 ttgaccaaat cgataatccc tcggctgatc ctgcctttcc acccatcttg atctggcgtg
     3001 ggcatcttga ggctttttcc accagcaaac gagtaggccc catagttttt tctacttgtt
     3061 gcctgctcat ggaagataag ggaatcctgc aggtcacgcc ccgtaatata atcaacaatc
     3121 tctcgtcgtt cgttactatc caaatcgagg atcgaagggt ccttcacatg acaatgatat
     3181 cctctccctc cactgaaata aagttccata tgtttttctt tgaacccgaa atcccctagt
     3241 aaaaactcat gtatcaattt aataaattct tttttgactt cgacaagtga ttcgacatac
     3301 cccatttttt ccgcgtttgg tagatgatcg gagtccaaat cgaagatcag ttcagcaccc
     3361 atccatattt tatcaggcat tttcgctgcg tccgggtgct gataatacgc cgatgaataa
     3421 tacgcatgct gaggagctcg gtcggtaagg aaattccaaa tcatctctcg tttctcaaaa
     3481 cccaggtgtc ggatcatccc tttcccacca aacaggagga aagcaaattc cctgcgtcca
     3541 aaacgatctg ggagctctat tttattagat gcgtaatagg tctggaacgt gttcttgagg
     3601 aattccattg atttttgtcc tgaattttca gctcccatgg tacgaaatca caatatccta
     3661 ctggtgtata atctttatcc atataaggag ataaagtttt attaacaaca ggcattaccc
     3721 tgtgctatgg gtagtattat cgaagatgag ttattctcgt cgtctgtcat taaggatttg
     3781 catgcgcttg attttgatta cgtcccaagc gagctacccc atagaaccga acaactgaaa
     3841 aaacttgccc agatgttcaa acccgttttt gtcgatatcg cacaaaatgt gctcatccga
     3901 gggtctgtcg gaactggtaa aacagcgatg acgaaatatt tctgtcagtc ccttgttcag
     3961 atcgcacgaa aacaaggaaa gatactcgaa tatgttcata tcaattgtcg aaaacgatcc
     4021 actgatgcga tggtcctcct tggtatcttg aatcattttg atgcacggtt ccctgaccgg
     4081 ggtttctcgg tgcaggaaat gttgcaagtt cttcgtacga acctccagcg gaaagaatca
     4141 catctcattc ttgtgcttga tgaggttgat gcgttggtaa aaaaaggaaa atcgaatctg
     4201 atttacgatc tcactaggtt taacgatgag accacaaaaa caggagcttc cgtctctgtt
     4261 attatgattt cacaaaagga tgttttcact gagctggaca cagcagcact cagtacgttt
     4321 aaacgcagca acaccatcat cctggaaaga tatacccgtg atgagttgta tgatatcata
     4381 caacagcgag tgcgacttgc attccatgca aatacggtcc gtgctgagag cgttgacctt
     4441 atcgctgata tcgccagtga atatggagac gcgcgatttt ccatagagct cctctggaaa
     4501 gcaggcatgt atgctgatga gaaacacgta aaacaggtgg tccctgagca tgtccgtgtc
     4561 gcaaaagcag aaacatactc ggttgttact gaaacaaaac taaagaatct agggaagcat
     4621 caattactta cgttgctctc aatagccaaa cgattacaaa aggaggagac cgcgtatgca
     4681 aacaccgggg aagtggagaa aacctatgag atcacctgcg aggagtattc tgaggacgca
     4741 cgggcgcata cgatgttttg gagctatctc aaagaaatcg aactcgcagg attcatcagg
     4801 gtgaaacctt ctgggaaggg ccaggtgggg aactcgcagc ttatctcgct gccagacatc
     4861 cctgctgagg tcttacgcag taaactagag gagcttctca cataagtggt ttaggggaca
     4921 ggtgctgcag tttggttttt tcttccagca gtactgtttt gctaattcta cgaggagcgc
     4981 atgaaattct ttgaaggcga gtattcgttc tgtttccggt atttttgatg aaagcccgtt
     5041 ttcaaaaaat tgttggatat ctctatagga atcactcaat acagagagcg ggactcgcag
     5101 gcagattcgt ttggtgtatg catcgatcac aaaaacagga tgatttccag cgtacagaag
     5161 aatcgaatcc gctgtttcag gaccgatccc tggaagggat aataattcat gtctgatctc
     5221 aggtgtatct cgcgagaaaa acctttggag atccccgtca tatttttttt caagatatgc
     5281 tgcgaagaat ttaagtcgct gtgccttttg gttgaagaac ccggagggct gtatcatggt
     5341 tttgaggttt ttttcctcca catgaatcat agcagggatt gtgaagatgc tttgttcctt
     5401 gaggttctta agagcttttt caacattcgt ccaggctgta ttctgagtaa ggatcgctcc
     5461 gatgatgatt tcaaaacgag gatcgctgtg ttgtctctga tggtacttcc tgtcgaccgg
     5521 ccaccagtcc tgatgcccaa aagtatctga taaaatctca tagagattct ttggtgaggt
     5581 cgccattgca actcatccta gctgtcttac aggttttttt cgacgatacc aaggatttct
     5641 gctgattttt gcatcgcgtt ttgcaggagt tcatgtaatt cagttgtaac ctgttggaca
     5701 tattcggtgt cttttatcga actcagatta acacgaacat tcaacatcgc agcttgtact
     5761 ccggtctgtg ccatcaaggc tgacaccgca gcatccgtga tcgaattttt gtttcccttt
     5821 actgcaacga tatgtgcgac atcaagaatc tggacacatt ttcgtgcggt ctccaaaggt
     5881 atttcagctg cttgtttaaa agcggtctgc agagcgttcc ttctcaactc cttttggtcc
     5941 tctgtctctt tagacatttt taacgctttc atgacttcat tgaacgcatc ggtgtcctta
     6001 tcaatgagat ccgtgaggtc ttttctgagc tcttcacttt tcccaagtat ctcccgtatc
     6061 tcatcctgta ctgtttcata gccttgtttt cctctcgtaa gattacacac cattgacgat
     6121 aacgcagccc cgagcgctcc tgataatgca gcaacactcc cgcctcctgg cgctgggctg
     6181 tttgaagcaa cctcatcaag gaacattttt atattctgtt tgacgagttt tgtcatgagg
     6241 attccttctg tatctctaac agtcgttttt ctagcacctg gtctacggtg aatttctcaa
     6301 gacggaggta atgatttgta ctatcgacca atgcttctaa tgggagtaga ccgacgatct
     6361 cgctaccgat gactgggact ccgaaccgct ctgcttcgtt ttttatggtc tcaaacaccc
     6421 gatagatcga tgttttttgg tagttcgtca gattcattga gacctgaacg atgccttttt
     6481 ctttaatctc aaatcctttc gctttcacgt atcggtatcc accactgcta tgacgtacta
     6541 cttttgctat ctcatctgcg atctttatgt tgcttgtacc taggttgaca ttgaatgcga
     6601 tgaggaaaaa gcgtgccccc acagcggtcg ctccagcggt tggatgaatc ttatttggcc
     6661 catagtccgg tactttctca gggtttttcc ctatctctgc tttgagtcct tcatattccc
     6721 ctcgtcgtac gtcagcaagg tttcgtcggc cttctcgttt cgctgcttcc tcgtataagt
     6781 aacaggggac attggttttc tc
//
LOCUS       DATMXJ010000068         4838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_69081, whole genome shotgun sequence.
ACCESSION   DATMXJ010000068 DATMXJ010000000
VERSION     DATMXJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69081"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..261)
                     /locus_tag="VN377_02045"
     CDS             complement(22..261)
                     /locus_tag="VN377_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27197.1"
                     /translation="MTSTTIKIQNDTKSQIDQYREYKNESYDEVIKKLIFIVKNLNQE
                     PELSKETIIAIESARERIKKGHFVSEEVAKHRLGL"
     gene            complement(469..924)
                     /locus_tag="VN377_02050"
     CDS             complement(469..924)
                     /locus_tag="VN377_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27198.1"
                     /translation="MRDKRFIAEHRGGPLKKEQHHQLIIWACDCSEHVLYLFGEKIDE
                     RLKNALKVAVEWKQGNASVGDARKASVCAHAVARESSNLIAIAVARSVGHAVATAHMA
                     DHSLGAAWYALKAVKNASKSVDAERKWQDKQLPSEIKELVLTARKSRKI"
     gene            1204..1635
                     /locus_tag="VN377_02055"
     CDS             1204..1635
                     /locus_tag="VN377_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27199.1"
                     /translation="MKKQLVVFGLIILLIGIALSGCVNESKKTMTYAKLMGDVKPLPP
                     TDLITLQSYQGGEVLYIEDTVFNVSYFEMSESQKYTFVSFGNSTSLLGFPLCIVGDKR
                     SEYPTGSLVNIPVHVKDYEINGTAVTWLEEWYTFYILQHPD"
     gene            complement(1712..3922)
                     /locus_tag="VN377_02060"
     CDS             complement(1712..3922)
                     /locus_tag="VN377_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWR27200.1"
                     /translation="MNGRPLLVSLDEPKTDRKGAIAVQKTHLSFAAFTDTHIGARYQD
                     PFFDTADHLDQLGEDLVDATNQLDFVVHLGDIVNHNTAQVNGEGLPWFVNQYTNNLKA
                     YLISHLNLPFYYVVGNHDVTDYQMNRGDPHNLTESLVDELSISTPVYAMMYEGILFLV
                     VPELGYVTWTHPVEYEWIDYMTSQYPTTTTVILCHQAIEDTTEEESHAPYRGKQDMDW
                     WATLFQNNPQIKMWIHGHNHYLDWYASNQSTGLTNAVRKFGHDMVFSSPYPQMDWYYH
                     HELDRVVIYNISSTGITTASWENNGAGGHWVSEYLHAWTIPTTFNTTSENWYSFPVFL
                     QDHETQLLDMKMLSPDITLQLIGTQPMELFFDARMESPNGSAKENILGFGNDQSGNVQ
                     WTHPGMRVYGSTTLTFPEKYPDNASVQEDGRSGQPYQSFPMGTISAAVPGQTYNFTIT
                     ARCASGMGRLVLNVSCCDWITRSQYSVLPGSESQVVSHTFGSCYETIHGVYTVPDNKN
                     AWFLQGSVEFLDSADYDVSLFSIKREQTSDTTDNFHLYLSSHWYNMTGSLGENEHINF
                     SVTPQGLCDHDGVMNFTAFIEGNRYGMVNLVYREPLLMGMNARFRVNSEKDNVFNLSL
                     TQTISRNSAEKMMIWDSALFQKFPHGTELFVRLLMNGAVGRVLYLILDKLNPGISTMF
                     KMFPFSTDPMYEQVILTADDDSEVRHQSTNGNIWFSSNCPGSQERSLKVWLPSR"
     gene            4435..>4838
                     /locus_tag="VN377_02065"
     CDS             4435..>4838
                     /locus_tag="VN377_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HWR27201.1"
                     /translation="MTKKNKQQIEEILSCPECGSGHLSKDYSRAELVCSGCGLVVDQD
                     IIDHGPEWRAFDNEQREKRSRTGSPMTYTMHDKGLSTTIGWTNRDAYGKAIPTKNRAQ
                     LYRLRKWQRRVRISDATERNLAVALSGIDRLSS"
BASE COUNT         1391 a         1036 c          918 g         1493 t
ORIGIN      
        1 ataaatgaca gtgtacattc actaaagtcc cagacggtgt tttgccactt cttcacttac
       61 gaaatgtcct ttttttattc gttctcttgc tgattctatt gctattatag tttctttact
      121 tagttctggt tcttggttta agttttttac aataaatatc agctttttaa ttacctcatc
      181 atagctttcg tttttatatt cgcggtattg atctatttgt gacttagtat cattttggat
      241 tttgatagtt gtagaagtca taatacacct tagtatacct atgtatacca tggtatatat
      301 aactttcgaa atgcatattt cttctgtctt atatttcatt aaaaaatcgt attattctcc
      361 tgttgcaaag aagcttacgg cctacaccca gcacatttcc agtcgtccat ctctcttaag
      421 attagatcag gcttccattc ctttttgctc atcagtttta ttatcttttt agatctttct
      481 acttttcctt gcagtgagga caagctcttt aatttctgat ggcaattgtt tatcttgcca
      541 tttcctttca gcatctactg atttacttgc gtttttcaca gctttcaaag cataccacgc
      601 tgctcctaat gaatggtcag ccatatgagc agtcgcaaca gcgtgtccaa cagaacgagc
      661 cactgcaatt gctatcaggt ttgaagattc acgtgctacc gcatgtgcac atactgatgc
      721 ttttcttgcg tcaccaactg aagcatttcc ttgtttccac tcgacagcca ccttgagtgc
      781 attttttaat cgctcatcta ttttttcacc gaagagatac aatacatgct ctgaacagtc
      841 gcaagcccat atgattaatt gatggtgctg ttcctttttt agaggcccac cccgatgttc
      901 tgcgataaat cgtttgtctc gcatttttgt ctttttcagt aatgttttgt taagttattt
      961 gtcttttcaa tcacctccat gcctccacgc tcataagtga ctgatagaaa ggaaaaaagg
     1021 aagaggggtt ttatccactt ccgtttcttt cgttcaccat caaagacgtt tctttggaat
     1081 ttgacttaaa aacgaccttt gtggctatgt cacggataac gggtatggcc tacattcagc
     1141 accagaaaat aattataagg taataactgt tactagaatt ataggtttga ggagaaagca
     1201 ggtatgaaaa aacaactagt agtttttgga ttaattattc tgctcatcgg gattgcgttg
     1261 agtggatgtg taaacgaaag taaaaagaca atgacctacg caaaattaat gggcgatgta
     1321 aaacccttac caccaacaga tcttattacg cttcaatcct atcaaggcgg tgaggtatta
     1381 tatatcgagg atacggtgtt taatgtctca tattttgaga tgtctgaatc acaaaaatat
     1441 acgttcgttt cttttggtaa ttctacttca ctactaggtt ttccactctg tattgtcgga
     1501 gataaaagaa gcgaatatcc tacgggaagc cttgtcaata ttcctgtcca tgtgaaggat
     1561 tatgagatca acggaaccgc tgttacttgg ctagaggaat ggtatacctt ctacatctta
     1621 caacacccag actaacatcc tccggtcata caccccgcac ctcagcatcg atattttaat
     1681 tatgaaacct tctaaaaata aagacaattg tttacctact tggtaaccaa actttcaagg
     1741 aacgttcctg agaacccggg caattggagg agaaccagat atttccattc gtactctgat
     1801 gtcgtacctc tgaatcatcg tcagcggtta ggatcacttg ttcatacata ggatctgtgc
     1861 tgaacgggaa catcttaaac atcgtagaaa tccctggatt caatttatcc agtatcaaat
     1921 aaaggacgcg accaacagca ccattcatca acaaccgaac aaataactcg gtaccatgag
     1981 ggaatttctg gaacagcgct gaatcccata tcatcatttt ttctgctgaa tttcgtgaga
     2041 tggtctgtgt aagactcagg ttaaacacat tgtctttctc gctgtttaca cggaaacggg
     2101 cgttcatccc catcagaaga ggttctcggt acacgaggtt caccattcca taccgatttc
     2161 cctcaatgaa cgctgtaaaa ttcatcacac catcatgatc gcagagtcct tgtggagtaa
     2221 cagaaaaatt tatgtgttca ttttcaccaa gagatccggt catattgtac caatggctac
     2281 tcaggtacaa atggaaatta tcggtcgtat cacttgtttg ttcccgtttt atactgaaca
     2341 acgagacatc ataatccgca gaatcgagaa actccacgga tccttgtaaa aaccatgcgt
     2401 tcttattgtc tggaaccgtg tatactccat gaattgtttc gtaacacgat ccaaatgtat
     2461 gagatactac ttgggattca ctcccgggaa gtacgctata ctgtgacctt gtaatccagt
     2521 cgcaacagct gacgttcagt accaatcgtc ccatcccaga agcgcatcga gcggttatag
     2581 tgaaattgta ggtttggcca ggcactgcag cagagatcgt ccccatagga aacgattggt
     2641 acggctggcc actccgccca tcttcctgga cagaagcatt atcagggtat ttttctggaa
     2701 acgtaagtgt tgttgatcca taaacccgca tccctggatg ggtccactgt acattccctg
     2761 attgatcgtt cccaaatcct aggatatttt cctttgcaga accattgggt gattccatac
     2821 gcgcatcgaa gaacagctcc attggttgag taccaatgag ttggagcgta atatctggtg
     2881 aaagcatttt catatccagc agttgtgttt catgatcctg gaggaacacc ggaaaagaat
     2941 accaattctc actagtagta ttaaatgtcg tagggattgt ccatgcatgg agatattctg
     3001 acacccaatg acctcctgcc ccgttgttct cccagctggc cgtggtaatg cctgtggaag
     3061 aaatattgta gatcaccact ctatctaact catgatgata gtaccaatcc atttgaggat
     3121 agggagatga aaacaccata tcatggccaa attttctgac agcatttgtc agtcctgtgc
     3181 tctgattgct tgcataccag tcaagataat gattgtgacc atggatccac attttaatct
     3241 gcgggttgtt ctgaaacagg gtcgcccacc agtccatatc ctgcttccca cgatacggcg
     3301 catggctttc ctcctctgtg gtgtcttcga tagcctgatg gcagaggata acggtcgttg
     3361 tcgtaggata ctgactcgtc atataatcaa tccattcata ctcaacagga tgcgtccaag
     3421 tgacgtatcc taactctgga acaaccagaa agaggatgcc ctcgtacatc atcgcataga
     3481 ctggtgtact aatactgagt tcgtcaacca atgattccgt aagattatgt ggatcccctc
     3541 tgttcatctg ataatcagtc acatcatggt tacccaccac gtaatagaac gggaggttca
     3601 gatgagaaat caagtatgct ttcagattat tcgtatactg attgacgaac caggggagtc
     3661 cttccccgtt gacttgcgct gtattatgat tcacaatatc tcccagatgc acaacaaaat
     3721 caagctgatt ggtagcatca accaagtcct caccgagttg atccagatga tctgctgtgt
     3781 caaaaaaagg gtcttgatac ctggcaccaa tatgcgtatc tgtaaatgcg gcaaaactca
     3841 agtgagtttt ttgtacagca atagcacctt ttctatcagt tttaggttca tccaaagaga
     3901 ccaatagtgg tctaccattc atttttattt cccttattat tctctgggaa ttaatatttg
     3961 gtgtcattat tgttataaaa gaaccaaaaa ttaaagctgt acaaatgaag acacttacta
     4021 ttttctttct catagactca aatctgttat actagtagta ctatacattt gttataatta
     4081 agtatatatc cttttttatt taaatattaa gacaaaaaat tatgtatcag ttttccacta
     4141 agttcttgaa ccatctctct gtctaacacc tcatgttttc ctcccgtaaa agaagattta
     4201 tgacctccgc ttatcattat tgggcaaaac aatataaagg taagaggagt atatctatat
     4261 ataaaaggaa tggagataaa agtttcacat ttttgttgac ccaaaacgtt tgagatttta
     4321 tttttgttct ttttgacatg ccatcatacc gaagatatca aaataaaaat ctatgatatg
     4381 aaccctttaa aagcactaat caacaaagaa ctatgtggtg gaggctgaat tcatatgaca
     4441 aaaaaaaata aacaacaaat tgaagaaatt ctcagttgtc ccgaatgcgg cagcggacat
     4501 ctgagcaagg attattctcg agcagaatta gtatgttcag gttgtggact agtcgttgat
     4561 caagatatca ttgatcatgg tccggaatgg cgtgcgtttg ataatgaaca acgggagaag
     4621 cgatctcgga ccgggtctcc aatgacctac accatgcatg ataaaggact tagtacgacc
     4681 atcgggtgga cgaacagaga tgcgtacggg aaagctattc caacaaaaaa tcgggcacaa
     4741 ctataccgac tacgaaaatg gcagaggcgt gttcgcatca gtgatgcaac cgagcggaac
     4801 ctcgcagttg ctctttcagg aatcgatcga ctctcatc
//
LOCUS       DATMXJ010000069         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_22110, whole genome shotgun sequence.
ACCESSION   DATMXJ010000069 DATMXJ010000000
VERSION     DATMXJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22110"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>2787
                     /locus_tag="VN377_02070"
     CDS             209..>2787
                     /locus_tag="VN377_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27202.1"
                     /translation="MKAKHSVWKTKLVKSMSLFTAILIAALFLGSAAGAGIAQNSSAT
                     INTKNIDQNINTNAKLQSPFKASIGLQGTPSEKNERLQKISQPTPLNPLEQVIRFNDG
                     TYYTGVGLTSGGTFEGAIRITPTEQSGYTGYGLIAVSWYHYGAMTHAGNIKIYASGTP
                     TEPGTLLQTVPFSVTGDGMKRIDLTSSVDLPITADMWVSVEVTHAAGEFPLGCDNGPA
                     IDGKGDMVFLPPGPWTELQIAAPTLDYNWMIDAVIDITTQYEHDVGVQEIVKPTSGVG
                     SAFTPEVKVKNFGLNDETAVPVNLKITRYDYTTLLEQHFDGTFPPAGWTRTNTKWQQS
                     STANAGGTAPEATFYYSPSETGTFRLYTGAIDTTGYSALSLTFRSMVNHYTTPYTLKV
                     ETSTDAVTWNTAWSISPTGSIPAHIETIPLTDGVGSSTFYISWTFEGYSYNINYWYID
                     DCLIQLVNPVEEYNETVLIDINSGVQQNVLFPNWLPDDLGVSENVNVDYKVEAQTMLV
                     GDNNTDNDYKTNMITLHFGYFHDIAVNGIVEPVDGTAEVFHPTVTVTNVGQNDETSVP
                     INLVISKPYMAQHFTGTFLPTDWTQEGVGEWMQSFTTNAGGTSPEARLYYANIVGNYA
                     YLDSPAFDTTGVTTLKLDFRNYIQNQYGGTYCKVLSRSDAGDTWTDITPWSNPINANV
                     PQAKYTIDISGDVGTATQIRFEFSGTSAGLYYWYLDDVTILQTDFTEYDQTVYVDLTS
                     GEVMDVIFTPDWTPSDLGTAENADIEYLMLAEAQLGTDGNAANDLLGKALTLHYPFFD
                     DVAVTQIIEPTDGLAQIQPVKVALANNGQNDESGEVTVVIEKFNGTAENFEGSDGGFV
                     RSGT"
BASE COUNT          851 a          603 c          600 g          733 t
ORIGIN      
        1 ttgtttcttc ctttatttag aaatgatttc taataagtgt attatataat ggtaatgtaa
       61 aatcctggga aatctcctta ttgatttatt tctttagaag tgatattgca attctttata
      121 tttaattcta actaataata tatatatatg gtgcgaaaca tttattaatg gcatctgcat
      181 ttaagagagt aaggcgaggt aaaattttat gaaagcaaaa catagcgtat ggaaaactaa
      241 gctagtgaag tccatgtcgc tatttacagc gattttaatt gccgccctgt ttttaggtag
      301 tgcagcaggc gcaggaatcg ctcaaaatag ctcagcgaca ataaatacaa aaaatataga
      361 tcagaatata aatacaaacg ctaaattgca aagcccgttc aaagcatcta tagggttgca
      421 aggcaccccc tctgaaaaaa atgaacggtt gcaaaagatt agtcaaccaa ctccactgaa
      481 tccattggag caagtgatta gattcaatga tggaacctac tacactggtg ttggtctgac
      541 ctctggtggt accttcgaag gagcaattcg gataacccca acagaacaat ctggttatac
      601 tggatatggt ctcatcgcag tgagctggta ccattacggt gcaatgacac acgcaggtaa
      661 tattaagatc tatgctagcg gtacaccaac ggaacctggt acattgcttc aaacagtacc
      721 gttttcggtc actggagatg gaatgaagcg tatcgatctt acatcctctg ttgatcttcc
      781 gatcacagca gatatgtggg tgtccgtaga ggttactcat gctgctggtg aatttccact
      841 tgggtgtgac aatggtcctg ccatcgatgg aaaaggagac atggtttttc tcccacctgg
      901 tccatggaca gaattgcaaa tcgcagcccc cactcttgac tacaactgga tgattgatgc
      961 agttattgat atcaccaccc agtatgagca tgatgttgga gtacaagaga tcgtaaaacc
     1021 aacaagtgga gtcggatcag cgtttacccc tgaagtgaaa gtgaagaact tcgggttaaa
     1081 tgatgaaacg gctgtaccgg tgaaccttaa aatcacaaga tatgattaca caactttact
     1141 ggagcaacac tttgatggga cgttcccacc agcagggtgg acgagaacca acacgaaatg
     1201 gcagcaaagt agtacggcta atgctggagg cacagcccct gaagcaacgt tttattattc
     1261 tccatcagaa acgggaacat tccgtttgta tactggtgct attgacacga ctggttactc
     1321 tgccttatca ttgacattta gatccatggt aaaccactac accacaccct atacgttaaa
     1381 ggttgaaaca tcaactgatg cggtaacgtg gaacactgct tggtctattt caccaaccgg
     1441 tagtatcccc gcacacattg aaaccatccc cttaaccgac ggtgtcggat catcaacgtt
     1501 ttacattagt tggacctttg aaggatacag ctataacatt aactattggt acatagatga
     1561 ttgtttaatc cagttagtaa acccggttga ggaatacaac gagacagtct tgattgatat
     1621 caactctggc gtacaacaga atgtgttgtt ccccaactgg ttacctgacg atttaggtgt
     1681 atcagaaaac gttaatgttg attacaaagt ggaagcgcaa accatgcttg ttggcgataa
     1741 caatactgac aacgattata aaacaaacat gatcaccctt cacttcggat acttccatga
     1801 tattgcagtc aatgggattg ttgaaccagt ggatggtact gctgaagtct tccatccaac
     1861 ggtgactgtt acgaacgttg gacagaatga tgaaaccagt gtcccaatta acctcgtaat
     1921 aagtaaaccc tacatggcac agcactttac tggtaccttc ttaccaacag attggactca
     1981 agagggagtt ggtgaatgga tgcagagttt cacgactaat gccggtggaa catcaccaga
     2041 ggcacgactc tactatgcca atatagtggg aaactacgca tatctggatt ctcctgcatt
     2101 tgacacaacc ggtgtcacaa cgctgaagct cgatttccgc aactatatac aaaaccaata
     2161 tggtggcacg tactgtaagg tattatcacg gagcgatgcc ggagatacct ggacggatat
     2221 tactccctgg agcaacccta ttaatgcaaa tgttcctcaa gcgaaataca ccattgatat
     2281 ctctggtgac gtcggaaccg caacccaaat acggtttgaa ttcagcggaa catctgccgg
     2341 cttatactac tggtaccttg atgatgtcac gatcctacaa acagatttca cggagtacga
     2401 tcagaccgtc tatgttgatc ttacatccgg agaggtcatg gatgtcatct ttacacctga
     2461 ttggacacca tctgatctag ggactgcaga aaacgccgat atcgaatacc tcatgttagc
     2521 ggaagctcaa cttggtaccg atggcaatgc tgcaaacgat ctccttggta aagcattgac
     2581 tctccactac ccgttcttcg atgatgtcgc agtaactcag atcattgaac caactgatgg
     2641 actagcacaa atccaaccgg ttaaggtcgc tcttgcaaac aacgggcaaa acgacgaaag
     2701 cggcgaagtc actgtagtca ttgagaaatt caatggcacc gcagagaact tcgaaggaag
     2761 cgatggtgga ttcgtccgat ctggaac
//
LOCUS       DATMXJ010000070         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_6517, whole genome shotgun sequence.
ACCESSION   DATMXJ010000070 DATMXJ010000000
VERSION     DATMXJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6517"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1171
                     /gene="rtcA"
                     /locus_tag="VN377_02075"
     CDS             152..1171
                     /gene="rtcA"
                     /locus_tag="VN377_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HWR27203.1"
                     /translation="MLTIDGSYGEGGGQIIRTAIALSVLTKQPIEITNIRSGRPIPGL
                     RPQHYTALSCIKTICDAEMEGLSVHSTNLRFTPHDIKPGTYTFDIGTAGSMTLVFQAC
                     LLSVFHASAPLTIILRGGTDVRWAPSWDYFSHVFLPLISKIGIQTETKLLRRGYYPTG
                     GGEATLTIHPITKLIPFIAEDPQSFSEISGIIHRANLPDHISTRMKHAAIKTAMRQNL
                     RSTIQIESMPSSSAGTGITLWSTSEQTILGSTILGEKTLSAEKVGETAANHLIQEISS
                     GATLDCYAIDQILPYLVLAPKGSTCLIREISTHTQTNMWLIKQFFSVDFEVTPQQNVH
                     RIVVK"
     gene            1259..2812
                     /locus_tag="VN377_02080"
     CDS             1259..2812
                     /locus_tag="VN377_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR27204.1"
                     /translation="MIKENIVEEKPTVTSADDRYHTLFERINAAAFLTSFEGQIQEAN
                     HKSYELFGYDWSELLRLTLQDVLSKDVDWKQCRDELAARGCLTFESETVCKNGSHFPV
                     DVNISIFRMDSSLVMFVLLWDITERKNQEKRLKESEKKYHGLFEYTTDGIFVLDVHGD
                     ILDINTRMCEILDIMKSSVLNKNLFNMGLLTARSLPVVIQQFEQLLSEKVAASYTTEI
                     KNRQGRVLEVEISSFFLVKKDNEVDNFVLIVRDTTARKESEQRQRQEHELFKALLENL
                     SDCIFFKDDQHRFILVNKTNAAYGNVVPEELNGKTDFDFLSKEQAQRIHDDEDAIMRS
                     GQAIVNKFERMAIDDEMERYLFVSKYPRYNDEGEIIGTMGLLRDVTEWKHTEDEYAKN
                     RVMLQTLLDAIPDAVCFKDCQNRFVLVNKAMTKRFMVQPEMMIGRTDFDFLPIEEAQK
                     ISEEDTRIIQSGTSMIDCVERIVEKDGSEHWYSVTKVAWNDQCGKIIGTLGLYRNVTD
                     EKKIQEIKQ"
     gene            3278..4291
                     /locus_tag="VN377_02085"
     CDS             3278..4291
                     /locus_tag="VN377_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWR27205.1"
                     /translation="MKKIFSVHILVYFLASVLLGTLFIGSSCQALTHNNTKYGMNEPP
                     TPPQQPTDGPGGSNYSHDGVKQSRYGFGVREFWIFEPSDPTPSSAPLIIFNHGWSAFF
                     PVFYKEWVVHLVKRGNIVVYPRYQLTPIIGTRSATQNAIHAVQQAIVILQNGDHVTPD
                     LDKFAIVGHSLGGGITAEMAALAADEGLPNPKAVMPIQPFIRLDTMMKDFHDIPASTL
                     LLVIVGENDTIVGNYSGKTIFTTADQIPLDRKDFIIQRTDRHGEPALVADHLAPVCIP
                     ESSLVDAMDYYSTWKLFDALTDYAFYGINQQYCLGNTPEQRFMGYWSDDVPVRELVIT
                     DTP"
     gene            4400..4588
                     /locus_tag="VN377_02090"
     CDS             4400..4588
                     /locus_tag="VN377_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27206.1"
                     /translation="MAKKKTNTKKTKSKNKTMYEEVVTVEDDLFDRCVRKGLIEKTAC
                     GYYFTPEFFETLDIYDDD"
BASE COUNT         1363 a          989 c         1036 g         1338 t
ORIGIN      
        1 ggttagtgta catcgcaata gcaaccaagg accacgttac agtcaaacga tatctcttct
       61 caggagaccg cttggccaat aaagaaagta cttgtaccgc aacactcgaa cttctcttag
      121 agctattgtc tcagaagaag agttgaaaaa cgtgctcaca attgatggat cctatggtga
      181 aggtggcggc cagatcatcc gcaccgccat cgcattatcg gtccttacaa agcagcctat
      241 cgaaattacc aatattcgat caggacgtcc gattcccggc ttacgcccgc aacattacac
      301 cgcactctca tgtataaaaa ccatctgcga tgcggagatg gaaggcctat ctgttcattc
      361 tacaaacctg aggttcaccc ctcatgacat caaacctggg acctatacct tcgatatcgg
      421 gaccgcgggg agcatgacac tcgtgttcca ggcatgtttg ttgagtgtgt ttcacgcatc
      481 agcacctctt accatcatcc tgcggggtgg aactgatgtc cgatgggcac catcgtggga
      541 ttatttttct catgtgttcc ttcctttgat atcgaaaatc gggattcaga ctgagacaaa
      601 attactcaga cgtgggtact atccgaccgg tggtggtgaa gcgacgctga caatccaccc
      661 tattacaaaa ctcattccat ttatagcaga agatccccag agtttttctg aaatatctgg
      721 tattatccat cgcgcaaacc taccagatca tatcagcaca cgcatgaaac atgctgcgat
      781 aaagacagcg atgagacaga atcttcgttc caccatccag atcgaatcta tgccttcgtc
      841 atcagctggc acgggaataa cgctctggtc cacatcagag cagacaattc ttgggtcaac
      901 aatacttggt gaaaaaacac tatccgctga aaaagtcgga gaaaccgcag caaatcacct
      961 gatccaggaa atcagtagtg gagcgactct tgattgttat gcaatcgatc agatacttcc
     1021 ctaccttgtt ctagcaccga aaggatctac ctgtcttata cgagaaatca gtacccatac
     1081 ccaaacaaat atgtggctca ttaagcaatt tttttctgta gattttgaag taacaccaca
     1141 acaaaatgtt catcggattg ttgtcaaata gaacacaaat ttaaatacaa acaatagatt
     1201 tgtttgttga acgaaacgaa ataaaaaata agatgcatat ctagtgatgg gaatgctgat
     1261 gataaaagaa aatatcgtag aagagaaacc aacagtcacc tccgcagatg atcgatatca
     1321 tacgttgttt gaacgaatta acgctgccgc atttctcacc agttttgaag gacaaatcca
     1381 agaggcgaac cataaatcct atgagttatt tggttatgat tggagtgaac tgctccgttt
     1441 gacattgcag gatgtcctct ccaaagacgt tgattggaaa caatgtcgag atgagctggc
     1501 tgcccgcggg tgtcttacgt ttgaatcaga gactgtctgt aaaaacggca gtcattttcc
     1561 ggtggatgtc aatatctcga ttttccgaat ggatagctcc ttggtaatgt tcgttttact
     1621 ctgggatatc acagaacgga agaatcaaga gaaacgtttg aaggaaagcg aaaagaagta
     1681 tcatgggcta tttgaatata cgactgatgg gatctttgtt cttgatgtac atggtgacat
     1741 ccttgatatc aacacccgca tgtgtgagat tcttgatatc atgaaatcct cggttctgaa
     1801 taagaacctg tttaacatgg gtctactcac agcacgatct cttcctgtgg tgattcagca
     1861 atttgaacag cttctctcag agaaagtcgc agcgagttat acgacagaga taaagaatcg
     1921 ccaggggcga gtcttggaag ttgagatcag ctcgtttttc ttagtcaaga aggataacga
     1981 ggttgataat tttgtgttaa ttgtccgcga taccacagcc cggaaagaat cggaacagag
     2041 acaacgccag gagcatgaat tattcaaagc cttgttggag aatctttctg actgtatctt
     2101 ctttaaggat gatcaacatc ggttcatttt ggtcaataaa acaaatgctg cttatggcaa
     2161 tgtcgtacca gaggaattga acgggaaaac cgattttgac ttcctatcaa aagaacaagc
     2221 gcagagaatc catgatgatg aggacgcaat catgcgttct gggcaagcga ttgtcaataa
     2281 gttcgagcgg atggctattg atgatgaaat ggagcgttat ctgttcgttt cgaaataccc
     2341 acgatataac gacgaaggag agataatcgg gacgatgggg ctgttaaggg atgtgaccga
     2401 atggaaacat acagaggatg agtatgcgaa gaatcgtgtg atgttgcaga cgcttttaga
     2461 cgcgatccct gatgcagtgt gttttaagga ttgtcagaat cggttcgtgc tggtgaataa
     2521 ggcgatgaca aaacggttta tggtccagcc tgagatgatg attggtagga cagattttga
     2581 ttttctcccc atagaagagg cgcaaaaaat ctctgaagag gatactcgga taattcagtc
     2641 tgggacatcg atgattgatt gtgtcgagcg gattgtcgaa aaagatggtt ctgaacattg
     2701 gtattcggtg acaaaggtcg cttggaatga ccaatgcggg aagatcatcg gcacgcttgg
     2761 tctttatcga aatgtgactg atgagaaaaa gattcaggag atcaaacaat aggatggaag
     2821 tattttcttc ttggttcttt tgagtgagaa tcccaagttt ttttctacta tcctctcttt
     2881 gtgattcgat aaagaattga gctttttctc gttataatag ataactgatg gaacaaaaga
     2941 caagtataac gggtattgta gggtttttct cctgttgaac tcttacgttc ttctttttct
     3001 tttcccttca aacgagtaag attctttcta gatatatcga gggaatccct ccttatgata
     3061 attttataac aaaatttata tactaactaa atcaatataa gtttaaggtt tgatgatggg
     3121 gggcgatccc tatttttccc atctctcctc accacctcct cccggcctcc ccatcttcac
     3181 cttacacaca cattcttttt tgaaacacaa tattccaacg ataaatttat attctgtttt
     3241 ctctctttct attcctatag gaaaacagag gtttcaaatg aagaaaattt tttcagtaca
     3301 cattcttgtg tacttcctag ctagtgtcct gcttggaaca ttgtttatcg ggtcatcctg
     3361 tcaagctctc actcataata ataccaaata tggtatgaac gaacctccga caccaccaca
     3421 acaaccaacg gatggtccag gcggctcgaa ttattctcat gatggtgtga aacaatctcg
     3481 atatggattt ggggtacgtg aattttggat cttcgagcca agtgatccta ccccatcatc
     3541 agctcctctc attattttca accatggatg gagtgcattc tttcctgttt tctataaaga
     3601 atgggtcgtc catttggtga aacgcggtaa tatcgtagtg tacccccgat accaactaac
     3661 ccccatcatc gggactcgtt cagcgaccca aaatgcgatt catgcggtac aacaagcgat
     3721 tgtcatcctc caaaacggcg atcatgtcac accagatctt gataaattcg cgatcgtcgg
     3781 tcattcactg ggtggtggta tcaccgctga gatggctgcc cttgcagcgg atgaaggtct
     3841 tccaaaccca aaggcagtca tgcctataca gccgtttatc cgtcttgaca cgatgatgaa
     3901 ggattttcat gatatccccg catcgacgct tttattagtg atcgtcggtg agaacgacac
     3961 cattgttgga aattattcgg ggaaaacgat ttttaccact gctgatcaga tacccctgga
     4021 tcggaaggat tttatcattc agcggaccga tcgtcatggg gaacctgcgc tagttgcgga
     4081 tcatctcgcg cctgtgtgca tcccggaaag ctctttggtc gatgctatgg attactacag
     4141 cacctggaaa ttgtttgatg cgttgactga ttatgcgttc tacggaataa atcaacagta
     4201 ctgtcttggg aataccccgg agcagcggtt catgggttat tggagtgatg atgtcccagt
     4261 ccgagagctt gtgattaccg atactccttg attttttccc aggatatact ttcgttttgt
     4321 cgttttcgca ggtgaggttt aaataggttc actggcaacg aatatcatgc tattagataa
     4381 caatagcagg atatgagaga tggcgaagaa gaaaaccaat acaaaaaaaa caaaatcaaa
     4441 gaacaaaacc atgtatgaag aggtggtaac cgttgaagat gatctttttg atcgatgtgt
     4501 acgaaaaggg cttatagaaa aaacagcctg cggttattac tttaccccgg agttcttcga
     4561 aacgttagat atctacgatg atgattaaca aagaagaatg tctttattcc ttttttttcc
     4621 tcttatcaac tcagtagatt atcgtttttt cttcagcttg agtttctgtc tcgtgtatgg
     4681 aagcaggcca ccagcgtcac agatcgccat gatctctttg gggagt
//
LOCUS       DATMXJ010000071         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53709, whole genome shotgun sequence.
ACCESSION   DATMXJ010000071 DATMXJ010000000
VERSION     DATMXJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53709"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VN377_02095"
     CDS             <1..916
                     /locus_tag="VN377_02095"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27207.1"
                     /translation="INDAPVVTDIPSQTIAEGSTFATITLDNYVSDVDNLDSEMTWTY
                     SGNVELTVSIVNRVATIIIPNEDWYGSETITFRATDPGALWDDDAALFTVTPIQDNLP
                     PNNPNRPSGPVSGTINVESAYTTNTTDPNNDQVYYMWDWGDGTQSSWLGPFNSGQSTQ
                     ANHSWEEKGSYDIKVKAKDTNNSESNWSEPCTVYILQKAFIFGMIRNAEPSGDLTTFE
                     PIRVRILWLSPFSISLQKTGLIIVTKNPFHGFIGGSIIFGRFYATPLTNATINPESAP
                     VRPNNSIQAEKTVKSTDNPLTPTSSPLI"
     gene            949..3186
                     /locus_tag="VN377_02100"
     CDS             949..3186
                     /locus_tag="VN377_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27208.1"
                     /translation="MIRKISLRILLVFIISSLMMSTTVSLANIRDSADQRSIQPITTS
                     DLMQLQKTKESSPQRADDSWWNSQWQYRKEIMVNYTKIVTNQTNFPVLLSFVSDAELS
                     VKAQPDGDDLVFTNANKGRLNHEIELFNCTTGQLIVWVNVTFLSSIQDTIIYLYYGNS
                     ICGNQENSQGTWGSHYRMVQHLSETTGIHNDSTMYGNDGLYYGSTQNSVGIIDGADQF
                     VGNFGATGGDYVDCGNDSSLNITDAITVSAWIKPNDQTQWNHICSKGNGEWTVNPNRV
                     YQLSIEINESIDFIINSNQTAGKAMTSQHVPIGSWSYVVGTYDRNAIRVYINGVECAV
                     KSPFAPLIQSNLVHFRIAARVADALNVGPPAFTFDGVIDEVRICDSALSAAWIATEYN
                     NQQSPMSFYSIGVEETLVSQPWDLTMHISTSMRSYDYVMFGEKTNASDGLDSYDVPKN
                     PPSIPPFLYTMFSTNLTEPYHMLWYDYKHCPDTYKVWDLKVQWVPSDYTSPTNVTIAW
                     NNSALNGSEYSSVTLENIETNETIDMLDMSSYTFNASALSLHPFKIICRALTRISNPS
                     YGWNFISLPFNQSLEKTNFFVKYAGRLYDWVNATAGGLVINSLAGWNRTSQNYYLTTT
                     LVPGEGYWMFFYHDCELWAQGVSTTESSNEISQLLPLWNTIGLPDDTLLPKEDLHVLY
                     ENILYNWTQATTNANPTGAPLILKFIYNWSRPIQNYVMCNFLLPGEGYWLYAYQQCVL
                     CKDNG"
     gene            3263..>3717
                     /locus_tag="VN377_02105"
     CDS             3263..>3717
                     /locus_tag="VN377_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27209.1"
                     /translation="MIGKKNWSIESRKKAGVLILGLIFIISSFVPGGTSSPQSKQTQN
                     GNQVLLTNEDNKYQDNLLNDKENLENQTDYLFTEEKSPPIPLDIPQTFYQTCGYWYRE
                     PTTYAQLISWYENLEQNFSNYMEVWKANEMYGLGQIPSDNYDMYYVRIT"
BASE COUNT         1140 a          764 c          791 g         1022 t
ORIGIN      
        1 tatcaatgat gctccggtcg taactgatat ccctagtcag acgatcgcgg agggttcaac
       61 ctttgcgacc atcactttag ataattatgt gtctgatgtc gataatttgg attctgagat
      121 gacctggaca tattcaggga atgtagaact caccgtaagc atagtaaacc gtgtcgcaac
      181 catcattatc ccaaatgagg attggtatgg ttctgagacg attacattta gggcgaccga
      241 tccaggagct ctgtgggatg atgacgctgc gctatttacg gtaactccga tacaagataa
      301 cctcccgcca aacaacccga atagaccatc tggaccggtt tcaggaacga tcaatgttga
      361 atctgcatac accacgaaca ccactgatcc taacaatgac caagtgtatt acatgtggga
      421 ttggggagat gggacgcaaa gtagttggct tggtcccttt aattctgggc aatcaacaca
      481 agcaaaccac tcctgggaag aaaaaggctc gtacgatatc aaagtcaaag caaaagatac
      541 caataactcc gaaagcaatt ggtctgaacc atgtacggta tacatccttc aaaaagcatt
      601 catcttcgga atgatacgca atgcagaacc ttctggtgat ttaaccacat ttgaaccgat
      661 acgggttcgt atcctctggt tatcaccatt cagtatctca cttcagaaga cagggcttat
      721 catcgtgaca aagaatccgt tccatggatt tatcggcggt agtatcatct ttggaagatt
      781 ctacgctaca cctttgacta atgctactat caatcctgaa tccgctccag tacgaccgaa
      841 taacagtata caagcagaaa aaacggtcaa atcaacggat aaccctctca cacccacctc
      901 ctcaccttta atttgaggaa ttaggaatta ctagggagtg aaagggttat gataaggaaa
      961 attagtctga gaatcctctt agtatttatc atatcaagtt taatgatgag tacgactgtc
     1021 tctcttgcga atattcgaga ctcagcggat cagagaagca ttcaaccaat tacgacttct
     1081 gatctaatgc aattgcagaa aaccaaagag tcatcaccac agcgggctga tgactcatgg
     1141 tggaattcac agtggcagta tcgaaaagaa ataatggtta attacacaaa aatagtaacg
     1201 aaccagacga atttcccggt cttactatcc tttgtttctg atgctgagct gagtgtcaaa
     1261 gcgcaacctg acggtgatga tctcgtgttt acaaatgcaa acaaaggcag attgaaccat
     1321 gagattgagc tatttaattg tacgaccgga cagctaatcg tttgggtgaa tgtcaccttc
     1381 ctttcaagta tccaggacac tataatatat ctgtactacg ggaatagtat atgcggaaat
     1441 caagagaaca gccagggtac ctggggctcg cattaccgca tggtgcagca tcttagtgaa
     1501 accactggga tacacaatga ttcgaccatg tacgggaacg atggtttgta ttacgggagt
     1561 acacagaatt ccgttggaat tatcgacggt gcagatcaat ttgtagggaa ttttggagcg
     1621 accggtggtg attatgttga ttgcggtaat gattcaagtc tgaatatcac tgacgcaatc
     1681 accgtctcag catggattaa accaaatgat caaacccaat ggaaccatat ctgttcgaaa
     1741 ggaaatggag agtggactgt aaatcctaat cgagtgtatc agttgagtat cgaaataaac
     1801 gagagtattg attttatcat caactcgaat cagaccgcgg ggaaggcgat gacaagccaa
     1861 catgtcccga ttgggtcatg gtcctatgtt gtgggaacct atgatcgcaa tgcgatacgt
     1921 gtctacatta atggtgtcga atgcgcggtc aaatccccat ttgcgcctct gattcaaagt
     1981 aaccttgttc atttccgaat tgctgctcga gttgctgatg cattgaatgt gggacccccg
     2041 gcattcacgt ttgacggggt cattgatgag gtccgtatct gtgatagtgc attatctgca
     2101 gcttggatag cgacggaata caacaatcaa caaagcccga tgagtttcta cagcattggt
     2161 gtggaagaga cacttgtttc tcagccttgg gatctgacga tgcatatctc cacctcgatg
     2221 aggtcatatg attatgtcat gtttggtgaa aaaactaatg catcagatgg tcttgacagc
     2281 tatgatgttc caaaaaaccc accatctatc ccaccattcc tctataccat gttttcaaca
     2341 aatttaaccg agccgtacca tatgctctgg tacgattaca aacactgtcc ggatacctac
     2401 aaggtttggg atttgaaggt acaatgggtc ccctctgatt atacttcccc aacgaatgtg
     2461 accattgctt ggaataattc agcattgaat gggagtgaat attcaagtgt gactttagag
     2521 aatattgaaa ccaatgaaac catcgatatg ctggacatga gtagttatac attcaatgct
     2581 tcagcactaa gtctgcatcc attcaagatt atctgtcgtg ctttaactcg catttcgaat
     2641 ccttcatatg gctggaattt catttctcta cctttcaatc aatcgttgga gaaaacaaac
     2701 ttttttgtca aatacgctgg aagattgtac gattgggtga atgcaacagc gggtggacta
     2761 gtgattaatt ccttagctgg atggaataga accagtcaga attactattt gacaaccacc
     2821 cttgttcctg gggaaggata ttggatgttc ttctatcatg attgtgagct ctgggctcag
     2881 ggagtcagca ccacagaatc ttcaaatgaa atttctcaac ttcttccctt gtggaatacc
     2941 attggattac ccgatgatac tcttcttccg aaggaagatc tccacgttct gtatgaaaat
     3001 atcctttata actggacgca agcgacgact aacgctaacc caactggggc accattgata
     3061 ttgaagttca tctacaattg gagtcgacct atacagaatt acgttatgtg caatttcttg
     3121 ttacccggtg aaggatattg gctatatgca taccaacaat gtgtactatg taaagacaac
     3181 gggtgagatt tcctttaaga cttcaattag aagggggaaa caaacaaaga aaatataaag
     3241 agaaatttga gaagggctac atatgattgg aaaaaagaat tggagtatag agtcgaggaa
     3301 aaaagctggt gttctaatac taggtttgat ttttattata agcagcttcg ttcctggtgg
     3361 cacgtcatca ccgcagagta aacagacgca aaatggaaat caagttctcc ttacaaatga
     3421 ggataataag tatcaggata atctccttaa tgacaaagaa aatcttgaaa atcaaactga
     3481 ttatcttttt actgaagaaa aatcaccccc cataccactg gatattcctc agacttttta
     3541 tcagacttgt gggtactggt atcgagaacc aaccacctat gctcaactga tttcttggta
     3601 cgagaatctt gagcaaaatt tctcaaatta tatggaagtc tggaaagcaa atgaaatgta
     3661 tggtctcggg cagattccct ctgacaatta cgatatgtat tatgtccgta tcaccaa
//
LOCUS       DATMXJ010000072        15814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_64204, whole genome shotgun sequence.
ACCESSION   DATMXJ010000072 DATMXJ010000000
VERSION     DATMXJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64204"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN377_02110"
     CDS             complement(<1..524)
                     /locus_tag="VN377_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27210.1"
                     /translation="MILALLNLILFVCGLIFLVPKNTWKKPFNKYKQIFAYHSVYLCL
                     IIVIVMIHLLEVNIIDTITTNMIGINFTPNIQFIEDGIVHWFSQLWTPAPVYFFVFVY
                     IVLYPFMLWFALFYFIMVDEKKAMKTFAYSLVGIYAIALPFYLFVPITNVYKYYGTPS
                     ALNMAIPAIEQFFYT"
     gene            complement(556..1110)
                     /gene="thpR"
                     /locus_tag="VN377_02115"
     CDS             complement(556..1110)
                     /gene="thpR"
                     /locus_tag="VN377_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWR27211.1"
                     /translation="MSIFRGFIAIDITATPQVLTFENEIAKTGADVKLVEPENIHITL
                     KFLGDTDEKYIDVIEQKIKESVRAVKPFPITLRGTGVFPNQKYVKIIWVGIIDNGQIE
                     PIARAIDTSLAPLGFKKEVRGFSPHLTIGRVKTAKNKEKLLNILEHYHEEEFTIQEVK
                     SIVLKKSELTPKGPIYTTLREVCL"
     gene            complement(1188..2021)
                     /locus_tag="VN377_02120"
     CDS             complement(1188..2021)
                     /locus_tag="VN377_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWR27212.1"
                     /translation="MICFDNHLHLRRDGRFLDAVREFQNAGGTHFILCQYPMPEIVLK
                     EKSYQTCYQETIHMAEEIRATIGIGVFVVVGPYPVDLLPLQEKFGRQTALSIIKKGMD
                     EAANLCREQKCIALGEIGRPHFPVDQQIIQDSNDLLTYGMQQAKDVGVPVVLHTESTT
                     PAQCKEFVEMGRKVGLTPDKIVKHFAPPLITHEENFGLMPSVLATRRNIVEALQKGTR
                     FLMETDYIDDMRRPGAVLAPKSVPKLTKKLMEENLLSEQQANLIHVENPQNTYHIDLK
                     E"
     gene            complement(2085..2462)
                     /locus_tag="VN377_02125"
     CDS             complement(2085..2462)
                     /locus_tag="VN377_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27213.1"
                     /translation="MKSSKSKQEPSPTVRCTVEKVAKTTGKNVKKTGGKRPLTIKPFL
                     NLHCTMNALRWILLLIFIYLAAGTVVVMIQDGTETLKNIVIYSIMGFFTFFMGYLGWI
                     LASDVLVIVSGKKNTNNEAEIET"
     gene            complement(2765..3958)
                     /locus_tag="VN377_02130"
     CDS             complement(2765..3958)
                     /locus_tag="VN377_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWR27214.1"
                     /translation="MQLGIVGKPNVGKSTFFNAATKAHAEVANYPFTTIEANRGVMYI
                     RKPCPCRDSHVTCTPHNSRCIDGIRFVPIEAIDVAGLVPDAHKGKGLGNKFLDDLRQA
                     HALIHIVDASGSTDAEGNPCELGAHDPLLDIEFLEKEINYWLFGIIKKSWEIIARKCE
                     LENKKIEKMLAEQLTGLGIRDEHITGAIRKINLDITKPSQWKDDQIFKLTDYVRAISK
                     PIIIAFNKSDKAPENLMEKRKELTKYIVIPCSAESELALSNAAEKGVISYTPGSNDFT
                     ILKKEELSEKQLKALEYIRTHVLQKYGSTGVQRCIDEAVRMLDLIVVYPVQDEHHLTD
                     KEGRVLPDAHLIPRGSTAKDLAYKVHTDLGNHFIRGIDARTHRIIGADHQLKDGDIIS
                     IIADI"
     gene            complement(3964..4845)
                     /locus_tag="VN377_02135"
     CDS             complement(3964..4845)
                     /locus_tag="VN377_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27215.1"
                     /translation="MFDETVIVTKGDVVIGDRSLVRFGIRTDGRIFVGEHAIIDGNLD
                     SPHDVRVDIFSSIGGDVRAGGNVYLGEKTKIKGALSLRGDLDVGDSVEIEKGFEAKGW
                     INIRNPVPMVIYIFVYLLQLLKMGRSEEIERILEELEQNDGDTIPISESFLFLPNNSI
                     MSISNSKVEGNLQVGKECKLLGNYDVKGNILVEEKTEVFGTLKATGNIFVGKKVKIHG
                     NIVSIGTVNITEQVDIIGSVSAEEISLSKTASIQGTFHAKKGISFVDSSKEQASEKVK
                     RFENDVDVVDEVKELLE"
     gene            5035..7062
                     /gene="lonB"
                     /locus_tag="VN377_02140"
     CDS             5035..7062
                     /gene="lonB"
                     /locus_tag="VN377_02140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HWR27216.1"
                     /translation="MSTKQTDGPPEQNEPPKEELPPIDVWIQNIKFTTTEDIKVPENL
                     LDQVIGQDQTVAIVKKAAEQKRHVMLIGDPGTGKSMVARAMTEFLPKGELEDIIVYPN
                     NEDTNTPTIRVVPAGKAHEIIRMQREEAKRKVEQQNSMILGIVLLIVTLSLIGAMVTS
                     HFEYAIFGVIAAVMVYLIVARGGFSQRKELQMIPKLLVSHEKNDVPPFIDATAAHSGA
                     LLGDVRHDPFQSAGLETPPHQLVEAGAIHRAHKGVLYIDEINTLSLPSQQHLLTAIQE
                     KKFQITGQSERSFGAMVKTEPAPCDFILVSAGNLDALNGMHPALRSRIRGYGYESYLY
                     SVMDDTQENREKLIRFVAQEVKKDGKIHHFNKEAVAEVIHEAQRRAGRKGKLSLRLRE
                     LGGLIRVAGDIAYERGDKIITQQHVIEAKHIARSLEQQVSDRALESQKSYRSFRTLGD
                     EVGVVNGLAVHSADPSMSEFSGLVLPIVAEVTPAGSQSEGKVIATGKLGDIAKESVQN
                     ISAIIKKYMGRDISKHDIHIQFIGTYEGVEGDSASISLITAVISAMENVPVRQDVAMT
                     GSISIRGTVLPIGGVTAKIEAAAEAGIKTVIIPKANLNDVLIEDKYKKIVEIIPVETL
                     TDVLQTALVGNGKEELLRKLKEMRPPKIIGKVELESERKTPAPLRSEEQKN"
     gene            7129..7629
                     /locus_tag="VN377_02145"
     CDS             7129..7629
                     /locus_tag="VN377_02145"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HWR27217.1"
                     /translation="MKALFIGRFQPFHRGHLLLLQRLATQYEEMIIGIGSSQYHDTPD
                     NPFSEAERIQMISQSLDAVGIHHYHIVSIPDIHDPPKWVDHVCSIVPDFDLIIANNPF
                     TRKLFSEKGFLVKGTTYFDRKRYSGKEIRRRMINDEPWDDLVPKVVYDIICKIDGIAR
                     LKRISH"
     gene            7702..8616
                     /locus_tag="VN377_02150"
     CDS             7702..8616
                     /locus_tag="VN377_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27218.1"
                     /translation="MSGSGEVIVHKIRLYLKLLRPFTLLAPLIVSSSVIIASLVSSQI
                     TDLPLVSLLCTLFSASFCFALLNGASNVLNQATDWREDALSKPYRPIPQGIVTSKEAF
                     QLSFFLYAGAFLLALTVHMIFFIFIILIALFSVTYSLTPRMKKFLFLNQLWVALPRGF
                     LAILSSWSVFGNPFEPLPLGMGCVAALFLFGGTTTKDILDAEADRTVGTKTLVNVFGL
                     KIAALFSLVFMIGAFSLIIPLVYFHILPTVVLPLSFLSFFSIIIGWLMLHRHKNSKYE
                     NTSAWTVMYMTYFLFAFSFAIITIFSSV"
     gene            complement(8591..11773)
                     /locus_tag="VN377_02155"
     CDS             complement(8591..11773)
                     /locus_tag="VN377_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27219.1"
                     /translation="MRRSNIVIVGILLLLVIPAVSLPGSSQGDGGPSWDTDYKEWSYR
                     QEIPLPIQTDENVSHYQPIDLRLRFENPCWTENENNTSIRIACWHADIWYDLESQIYS
                     LEKIPGETSFIGECNVIFLVPAFADGTEQYFMYYDDEKTPRPDYIDHVTIEDLNYSAS
                     PLPEISAVARFYGITEDGYCIYGVGQEGQLLDRSCAQVVVKQKKECKEFDIIDSDQIV
                     SFAFSYYYGDKEKDESSSDQVFLDKKIFIDGNLMVEFGIFSESKKKDVQTTAIYRYYY
                     SPIEEKRINVHVKHEMLKDATVQGIDNIDGRFGSLISLKSRSAAVDSLNFGNIYPYLN
                     FYSEKDKVEQYQLNQNPSTKEREWIISYKNDATLGSEAWLCYGYGKKGIANAVLFSSN
                     QGVVTSGTDERDGIQLKVAEKEYVNFLGTEVDYVSINFGRHSYVPGHSHDVTIPANLI
                     VQFDAEVFASETGGYESVQKESYFYKSLIQSRRYSGDIPFVREQKRYNVTIIPRLGRT
                     HLSHPWLWNLTGSVFPVMWVELHQGERLVAEGAADRSLLMRAKKTFSSVLEGDYLVKI
                     FFKWGNKKIFTGSTVIHLNKDTRVNVFCTWERTITFTFLDQNGKGIPGIHGWLTNKDG
                     ILYDENITQNNGELLLKAPYDGSDPYILQADYKSLTIYDGRLPKTLRKLNKQITLELY
                     DFNVVITDDLGLSPGVELTPVLVTPDGNKTVQITPKEKTKGSFVFENVPAGDYHLQIS
                     FGEFIDDLHVTIPNAGNVVHMKFSAIFQVTIDLFDSKGNTLVNDNIEFLIVRDNQTIA
                     KTKEKSITLPPARYTINAYLDNELIGVKTVELTNDKHLTFVTTIESLFPLFLSLFFYV
                     LFGCLVILTILKKFSLSSLLKSLAILLVILSFFQPWWLFTGSSSTLPAEKTTALYVNP
                     GMMIETKTFYGEVSLSLAEMPDIFLMFLGAIVPLAALVCICIGIGIVLKRTKKKNYAF
                     LLGIVGVILMGIILFAFTFGTTRLIETSLGSVQGEDVLSVSMGSEDVMMQASWGFSVG
                     FYIVCIALIVALIAVLLDIRMRLTQKKKLL"
     gene            complement(11912..12877)
                     /locus_tag="VN377_02160"
     CDS             complement(11912..12877)
                     /locus_tag="VN377_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27220.1"
                     /translation="MSVKLTMKEPYTKGRFLSKKFRGYFDLLRPFTLLAPFFVSMFIM
                     FASLVYNDKFVEFPNWWIVIGQASLTLAFLNGASNALNQATDVEADKISKPYRPIPRG
                     VVERDEAQSIAYILYLFALLRAVTINTWFGVFVFLIMLFTVIYSLPPRMKRFLFINQV
                     WIAIPRGLFAILASWSVFGDPFEIVPLIIGLIGLTYFIGAMTTKDIVDCEADRKTGTR
                     TLINTYGTRKAAFISLPFLIIPFAMFPVLIHYQLIDPYFLPLTLFIIPGFVIFYLMLK
                     QQQSGTLENVSAWSLMYITYLFYAIGFTALIILGKLGQLNFFTVI"
     gene            complement(12995..14380)
                     /locus_tag="VN377_02165"
     CDS             complement(12995..14380)
                     /locus_tag="VN377_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR27221.1"
                     /translation="MNKKKVCIIGAGIGGLTAGALLLKKGYSVDIFEKEPIVGGRALS
                     LEMSSQTLESYKQTLAKFNTAIVFSEPPLEEIFKNKMFENYTLDLGFHMIGSGIVVKL
                     RDILKEYFDNITMYKSRLYEQKNNHFGYFVTTADKISMLPNIVRLLLSGEKTMKDLDA
                     VSMTETIKKYGKGKMKIVLEVNPRLISTINNLDLISTGEVFRTQKDMKLGGVNYPKHG
                     LLKISQTIAEYIEKNGGKIHLKSPVSKILIKNKKAEGVVVADKEYMFDIVLSNMLVQN
                     LFTIADEHQFPKEYVTTMKTLEGTGSLCAYYSLNQIDPRLLGKSFIFIERDIGVDGND
                     AVGMIDFITAMEETGLSPSSHYLVQSYIICTPKEAKDKKILNKLKEALDKNLERIIPQ
                     YHSQLQWAIYPSVWHLDGVAKTLVNTKPDIQTPIENLYIIGDCVKAPGIGINCAINSA
                     RIIQEMLSKSV"
     gene            complement(14402..15616)
                     /locus_tag="VN377_02170"
     CDS             complement(14402..15616)
                     /locus_tag="VN377_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HWR27222.1"
                     /translation="MKYDVTIVGAGPAGSTTAKFLAEKGFKTLLLDKEKFPREKPCGG
                     GLPIRVLQRFPYVINDNIIEAYSSSGTIFSPSLQHQIEINKNTPIIAMVLRKKFDFEL
                     VKFAIEAGAIFQEATPASAVRISNDTAQVTLDLGRKIDSEIIVGADGVNSTLVRNMGL
                     RASRTEKGICILQEFEVDEKIMDEYFKKSRHCYIHSRFKSASGYGWVFPKKEHLNIGF
                     GIINMDKNQQPKHNLLNWYQDYITLLKEKNLIPQHLKETPVKGGALLTHPLEKTYADR
                     FLLVGDAAGFINPLTGEGIYYAMVSGQIAAEIITEALEKKQTTQEFLSQYQTRWQNDF
                     GKDINLILKVVQRGSMEYVEKVFKIAYKDPVLTDLMMGVITGQLSVQQYKWKIVRRFF
                     YSSLKNRLHLLK"
     gene            complement(15613..>15814)
                     /locus_tag="VN377_02175"
     CDS             complement(15613..>15814)
                     /locus_tag="VN377_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27223.1"
                     /translation="HTSMSKGAAYAKLLRVPGIGALGIVPVIAALTMGITDFYPLFLI
                     FIIYSNQECIEMKTGFHDLGNL"
BASE COUNT         4344 a         3237 c         3234 g         4999 t
ORIGIN      
        1 gtatagaaga attgttctat ggctgggatg gccatgttta acgctgaggg tgtcccatag
       61 tatttgtaga cgttcgtgat tgggacaaat agataaaatg gtagtgcgat agcgtaaatc
      121 ccgaccagtg aataggcgaa tgtcttcatt gctttcttct catcaaccat gatgaaatag
      181 aagagtgcga accagagcat aaatgggtaa agaacgatgt agacaaagac aaaaaaatat
      241 acaggtgctg gcgtccaaag ttgtgaaaac caatgcacaa ttccatcctc gatgaattgg
      301 atgtttggcg taaagttaat accgatcatg ttcgtcgtga tggtatcgat gatgtttacc
      361 tcaagaagat ggatcatgac aataacaatg ataagacaaa gatagacgga atgatacgcg
      421 aagatctgtt tgtacttatt aaagggtttt ttccaagtgt tttttgggac aaggaaaatc
      481 agaccacaaa caaataagat gagatttaga agtgctaaaa tcattaacca ggtttctatg
      541 gtttcaccgc cgtcctcata aacatacttc tcttaatgtt gtgtagattg gtcctttcgg
      601 ggtcagctcg cttttcttaa gaacaataga ttttacttcc tggatcgtga actcttcttc
      661 atggtagtgt tctagtatgt tgaggagttt ttctttgttt ttcgctgttt tcacgcgacc
      721 tatggtcaaa tgcggagaaa accctctcac ttcttttttg aatccaagag gggccagcga
      781 ggtgtcaata gctcgtgcga taggttcgat ttggccgtta tcgatgatgc cgacccagat
      841 tatttttaca tacttttgat ttggaaaaac accggtccct cgcagcgtga ttggaaaagg
      901 tttgaccgct cttactgatt cttttatctt ttgttcgatg acatcgatgt atttttcatc
      961 ggtatctcca agaaatttta gtgtgatgtg tatgttttct ggttcaacta atttcacatc
     1021 agcacctgtt tttgctatct cgttttcaaa tgtgagtacc tgcggtgttg ctgtgatatc
     1081 gattgcgatg aaacctcgaa agattgacat gatgtcctcc aattgatatc gataaacaat
     1141 ttccctctat ttagcagtat ttcatccttg gtcgttatgg ttcttatcta ctcttttaaa
     1201 tcaatatgat aggtgttctg tgggttttct acatgaatca gattcgcttg ttgctctgat
     1261 aggaggtttt cttccataag tttttttgta agtttaggga ctgattttgg ggcaagcaca
     1321 gcaccgggtc gtcgcatatc gtctatgtaa tctgtctcca ttaagaatcg tgtgcctttc
     1381 tgtaatgcct caacgatatt tcttcttgtt gctaggaccg atggcatgag accaaaattt
     1441 tcctcatgtg ttattaacgg aggggcgaag tgtttgacta ttttgtctgg ggttaaaccg
     1501 acttttcttc ccatttcaac gaactctttg cattgtgcag gggttgtact ttctgtgtgt
     1561 aaaacgaccg gtactccaac atcttttgcc tgctgcatcc cataggtcaa taaatcgttt
     1621 gaatcctgga tgatttgttg gtcgacagga aaatgtggtc gtccgatttc tccaagagca
     1681 atacattttt gttctctgca gaggtttgcc gcctcatcca ttcctttttt gattatcgaa
     1741 agggctgtct gtcttccgaa tttctcttgc agtggaagca ggtcgaccgg gtatgggccg
     1801 acaacaacaa acaccccgat tccaatggtc gcgcgaatct cctctgccat gtggatcgtc
     1861 tcctgataac aggtctggta acttttttct tttaagacta tctcgggcat cggatattga
     1921 cacagaatga aatgagtacc tccagcgttc tggaactctc ggactgcgtc cagaaatcta
     1981 ccgtctcgcc gaaggtgaag atgattatcg aagcaaatca cgtctcatac ctcaaagaga
     2041 taataaaaaa gaatgatata aagaggtaga gtaagcattt ttgcttatgt ctctatctca
     2101 gcttcattgt tggtgttttt ctttccagat acaatgacga gaacatcact ggcgagaatc
     2161 caccctagat accccatgaa gaacgtgaag aaccccatga tactatagat gacaatgttc
     2221 ttcaatgtct cggtcccatc ttggatcatt acgacaaccg tgccagctgc taggtagatg
     2281 aagatgagca gtaagatcca tcgtaacgca ttcatcgtgc agtgaagatt cagaaacggt
     2341 tttattgtca gtggtctctt tccacctgtt tttttcacat ttttcccggt tgttttagct
     2401 actttttcga cagtacaccg gactgtcggt gaaggttcct gttttgattt tgatgatttc
     2461 atatcatgtt tttgggtagg tttgttcaga cggtatcttt tatgaaaagg tgctaaggga
     2521 agtacgcgta agggtatttt tctagttttt agatttcgcg agagtctttt attgcttatt
     2581 tttcttattg acaccgttct acctcctagt gaagaatatc ttttgagtgg tcccaaaaaa
     2641 gaaacagatg gggactataa aaggctgttt tgtagatacc tatgcatcta cggtgcagct
     2701 cattatccct tcgcgatgca accttgtatg cacctacatg ttcggcaagg tatcccgcac
     2761 gttgttatat atctgcgatg atgctgatga tatcaccgtc ttttagctga tggtccgcac
     2821 cgatgatgcg atgggttcgt gcatcgatac ccctgatgaa atgattcccg aggtctgtat
     2881 ggactttata ggcaaggtct tttgcagttg acccgcgtgg aattaagtgc gcatcgggga
     2941 gtactcgtcc ttctttgtcg gtgagatgat gttcatcttg tacggggtag acaacgataa
     3001 gatcaagcat ccggaccgct tcgtcgatgc atcgctgaac accggttgat ccgtattttt
     3061 gaaggacatg tgtcctgatg tattccaatg ccttgagttg cttttcggag agctcctctt
     3121 tttttaggat tgtgaagtca ttactccctg gtgtatagga gatgactcct ttttctgctg
     3181 cgttgcttaa tgcaagttca ctttcagcgc tgcaggggat aacgatgtat tttgtcaatt
     3241 ctttcctttt ttccatgagg ttttctggtg ccttgtcact tttgttaaac gcgatgatga
     3301 ttggtttgct gattgctcgt acgtagtcgg tgagtttgaa aatctgatcg tctttccatt
     3361 gtgatggttt tgttatatcg aggttgattt ttcgtatcgc tcctgtgata tgttcatctc
     3421 gtattcctaa tccggtgagc tgctctgcga gcattttttc tatttttttg ttttcaagct
     3481 cgcattttcg tgcaataatc tcccaacttt ttttgatgat tccaaacaac cagtaattga
     3541 tttctttttc aagaaattcg atgtctaaga gtggatcatg ggcacctagt tcacaggggt
     3601 ttccctctgc atctgtgctt cctgatgcat cgacgatatg gatcagggca tgtgcttgtc
     3661 tgaggtcatc aaggaattta tttcctaacc cttttccttt gtgggcatct gggactaaac
     3721 ctgcgacgtc aattgcctcg ataggtacga atctgatccc atcgatacat cgtgagttat
     3781 gaggggtaca ggtgacatga gaatctcgac agggacatgg tttacgtatg tacatgacgc
     3841 cgcgatttgc ctcaatggtc gtaaacggat aatttgcaac ctctgcatgg gcttttgtgg
     3901 cggcgttgaa aaaagtggat tttcctacat ttggtttccc aactatacca agttgcattt
     3961 tttctactcc aaaagttctt ttacttcatc gacaacatcg acatcgtttt caaagcgttt
     4021 aaccttttca gacgcttgtt cttttgaact gtcaacaaag gagatgcctt tcttcgcatg
     4081 gaatgttcct tgtatcgagg ctgtttttga tagtgagatt tcttccgcgg agacactccc
     4141 gatgatatcc acctgctctg tgatattgac cgtgcctatt gatacgatat tcccatggat
     4201 ttttaccttt ttaccaacaa agatattgcc agttgctttc aatgtgccga agacctcagt
     4261 tttctcttcg actaagatgt tgcctttcac atcgtagttg cctaatagtt tgcattcttt
     4321 gccgacttga agatttcctt ctacttttga atttgagatg ctcatgatgg agttgtttgg
     4381 taaaaagaga aatgactctg atattggtat ggtgtccccg tcgttttgtt ctagttcttc
     4441 caggatacgt tcgatttctt cgcttcgtcc catttttaac aactgtaaaa gatatacaaa
     4501 gatatatata accattggaa ctggattccg aatattaatc catccctttg cttcgaatcc
     4561 tttttcaatt tcaacactat caccgacatc aagatcgccc ctgagcgaca aggctccctt
     4621 gatcttcgtc ttctccccta aatacacatt tccaccggct cggacatccc ctccgatcga
     4681 agaaaaaata tccacacgga catcatgagg cgaatcaaga ttcccatcga taatcgcatg
     4741 ttcaccaaca aaaattcgac catcagtacg gattccaaaa cgaacaagcg aacgatcacc
     4801 aataacgaca tcacccttgg tcacaatcac agtctcatca aacaaggtct tatctggaat
     4861 tacaagcgtt tttctgtcaa aagccattat ccaaggatat gaactatcgc attataaaac
     4921 caacggaatc gccatttccc ggaaagaaaa aaattgatat atgaaatgcg tatatgtcgg
     4981 taaatctcaa atgagagcgt ttcatcacgt tcttgttgag tggaagtttt aaccatgtca
     5041 acaaaacaaa cagacggacc gccagaacaa aatgagcccc caaaggagga attacctcca
     5101 attgatgtct ggatccagaa cataaaattt acaaccactg aggatatcaa agtcccagaa
     5161 aacctcttag atcaagtcat cggacaagat caaacagtcg ctatcgtaaa aaaagcagca
     5221 gaacagaaac gacatgtcat gctcatcgga gacccaggaa ccgggaaatc aatggtcgct
     5281 cgcgccatga ccgagttctt accaaaagga gaactcgaag atatcatcgt ctaccccaac
     5341 aacgaggata ccaatactcc aaccatccgg gttgttcccg ctggaaaagc acatgaaatc
     5401 attcgaatgc aacgagagga agcgaaacga aaagtcgaac aacaaaatag catgattctt
     5461 ggtattgttc tgttgattgt aaccctctcc cttataggtg caatggtgac cagtcatttc
     5521 gaatatgcga tttttggtgt gattgccgca gtgatggtct atcttattgt cgcccgaggt
     5581 gggttttctc agagaaaaga attacaaatg attccaaaac tcttagtttc acacgaaaaa
     5641 aacgatgttc ccccattcat agatgcaacc gctgcacact ctggtgcgtt acttggtgat
     5701 gttcgtcacg atccgtttca atccgcaggg ttagaaacac ctccccatca actggtagaa
     5761 gctggagcca ttcatcgggc gcacaaggga gtgttgtaca tcgatgagat taatacctta
     5821 agtcttcctt cacaacagca tctacttacc gccattcagg agaaaaaatt tcagattacc
     5881 gggcagtctg aacgaagttt cggtgcaatg gtaaagacag aacctgcgcc ctgtgatttt
     5941 atcctggttt ctgcagggaa cctcgatgcg ttaaatggca tgcatcctgc gttacgctca
     6001 cgaatacgag gatatggata tgaaagttat ctttacagcg tcatggatga tacccaggaa
     6061 aatcgggaaa aactcatcag attcgtagcc caagaagtaa agaaagatgg aaaaatccat
     6121 catttcaata aagaagcagt cgccgaagtc atacatgagg cgcaacgcag agcaggccgg
     6181 aaagggaaac tctcattacg attacgtgaa cttggtggtt tgatccgagt cgcgggagat
     6241 atcgcttatg agcgaggaga taaaattatc acacagcagc atgtcattga agcaaaacat
     6301 atcgcgcggt cattagaaca acaagtctca gatcgagcat tagagtcgca aaagagctat
     6361 cgttccttta gaaccttggg ggacgaagtc ggtgtggtca atggtcttgc ggtgcattct
     6421 gctgatccct cgatgagtga gttctctgga ttagtcctcc cgattgtcgc ggaagtgaca
     6481 cccgcagggt cacagtcgga aggaaaggtg atagccaccg gaaaacttgg agatatcgcg
     6541 aaagaatcgg ttcagaatat ctcagcgatc attaaaaaat atatgggacg agatatctct
     6601 aaacatgaca ttcatattca atttatcggc acctatgagg gcgtcgaggg tgactcagca
     6661 agcatttctt tgattaccgc agtcatttct gcgatggaga atgtcccggt aagacaggat
     6721 gtcgccatga ccggttcgat cagtattcga ggaaccgtgc ttcccattgg tggtgtaaca
     6781 gcaaaaattg aggccgctgc tgaggcaggg attaagaccg taattattcc gaaagcgaat
     6841 ctcaatgatg tgttaattga ggataaatac aagaaaatag ttgaaatcat ccctgttgag
     6901 accttaacag acgttctcca aaccgcgctc gttggtaatg ggaaagaaga gctcttacga
     6961 aaattaaaag agatgcgtcc tccgaaaatc ataggaaaag tagaactgga atcagaacgg
     7021 aaaaccccag cgcctcttcg ttccgaagaa caaaagaact agaactgtta ttgggactta
     7081 tgattataga atgaaaagaa tctcacttta caaattcgta ggtctaatat gaaagcgttg
     7141 tttatcgggc gttttcaacc cttccatcgg ggtcatctcc tgttgttaca acgattggct
     7201 actcagtacg aggagatgat cattggcatt gggtcttctc aatatcatga cactcctgat
     7261 aatccgtttt cagaagcaga acgaatccag atgatttcac aatctttaga tgccgtaggt
     7321 attcatcact atcacatcgt ctctatacct gatatccatg atcctcccaa atgggttgac
     7381 catgtctgtt ccattgttcc agattttgac ctgattattg caaataatcc tttcacaaga
     7441 aaattattct ccgagaaagg gtttctagtg aagggaacca catattttga tcgaaaacgg
     7501 tattctggaa aagagattcg cagacggatg atcaatgatg agccttggga tgatctagtt
     7561 cctaaggtcg tctatgacat tatctgtaaa atcgatggaa ttgctcgtct taaacggata
     7621 tcacattaat tcttctaaaa aagatttttg ataagaaatg taaagatata tttatataaa
     7681 ataacgaagc accatatcat tatgagtggt tcgggtgagg ttattgtaca taagatacgc
     7741 ctatatctaa aacttctcag gccgtttacc cttctagcac ccctcattgt atccagcagc
     7801 gtcatcattg caagtcttgt aagttcccag attactgatc tcccgcttgt ctctctcctt
     7861 tgtaccctat tttcagcaag tttttgtttc gcattgctca acggtgcttc gaatgttcta
     7921 aaccaagcga cagattggag agaagacgct ttatcaaaac cctaccgtcc gattccacaa
     7981 ggaatagtta catcaaagga agcctttcaa ctttcatttt tcttatatgc cggggcattt
     8041 ctcctcgctc tcactgtcca tatgatcttc tttattttta tcatcctgat tgctttgttc
     8101 tctgtcactt actctcttac accgcgcatg aaaaaattct tgtttcttaa ccagctgtgg
     8161 gtggcgctcc cacgaggttt tttagcaatt cttagttcct ggagtgtgtt cgggaatccg
     8221 tttgaaccac ttccgctagg tatgggatgt gttgcagcgt tatttctttt tggcgggact
     8281 acaacaaaag acatattaga tgcggaggca gatcggactg ttggtacgaa aaccttggta
     8341 aatgtctttg gtttgaaaat agctgccctc ttctccctcg tattcatgat tggagcattt
     8401 agtttaatta ttccgttggt ctattttcat attcttccaa cggtcgttct tcctctctca
     8461 tttcttagtt tcttcagcat cattatcgga tggctcatgc ttcatagaca taaaaattca
     8521 aaatacgaaa acacatcggc ctggaccgta atgtatatga cctattttct ttttgcattc
     8581 agttttgcga ttataacaat tttttcttct gtgtaagcct cattcggata tcgagaagta
     8641 ccgctatcaa tgctacaatt agtgcgatac acacgatata gaacccaacg ctaaatcccc
     8701 aacttgcctg catcatcaca tcttctgaac ccattgatac agatagcaca tcttctcctt
     8761 gaacagaacc aagacttgtt tcaattaatc gtgtcgtccc aaatgtaaat gcgaataaaa
     8821 tgattcccat gaggattact ccaacgatac caagcagaaa cgcataattt tttttctttg
     8881 ttctttttaa aacgattcca atacctatac agatgcacac aagcgcggca agaggcacta
     8941 tcgcccccag gaacataagg aagatatcag gcatttccgc taaactgagt gacacctctc
     9001 cgtagaacgt ttttgtttca atcatcatcc ctgggttcac gtataacgct gtagtctttt
     9061 ctgctggaag tgtcgaactc gagccggtga ataaccacca gggttggaag aatgataaga
     9121 tgacaagaag gatcgcgagt gattttaata acgatgatag tgaaaatttc tttaatatcg
     9181 ttaggataac aaggcaacca aaaagcacgt aaaaaaagag ggaaagaaag agggggaaca
     9241 atgactcgat tgtcgtcaca aacgtcagat gtttatcatt ggttagttcc acggttttta
     9301 ccccgatgag ttcgttatct aaatacgcat tgatcgtata tcgggctggg ggaagtgtaa
     9361 tgcttttttc cttggttttg gctatggttt gattgtcacg aacgattaga aattcaatgt
     9421 tgtcgttcac caatgtattt cccttggaat cgaataagtc aatggtgacc tgaaaaatag
     9481 ctgaaaattt catatgaaca acatttcctg cattcgggat tgttacatgc aaatcgtcaa
     9541 taaattcccc aaaacttatt tgaagatgat aatctcctgc agggacattc tcaaaaacaa
     9601 atgatccttt tgttttttct ttcggggtta tttggactgt tttattgcca tctggtgtga
     9661 caaggaccgg tgtcagttca actcctggtg ataatccaag atcatctgtg ataacgacat
     9721 tgaaatcata gagttccaag gtaatttgtt tatttaattt tcttagggtt tttgggagcc
     9781 ttccgtcata gatagttaaa cttttgtaat ctgcttggag aatataggga tcgcttccgt
     9841 catagggtgc cttgagaagt aattctccat tattttgtgt aatgttttca tcgtacagaa
     9901 taccatcttt attggttaac cacccatgaa ttcctggtat cccctttccg ttttgatcaa
     9961 gaaatgtgaa tgttattgtc cgttcccagg tacaaaaaac atttactctt gtatctttat
    10021 tcaaatgtat tactgtcgac cctgtgaaaa tcttcttatt gccccacttg aaaaatattt
    10081 ttacaagata gtcgccttcc aagacacttg aaaaggtttt ctttgctctc attaaaagag
    10141 aacgatctgc agctccttca gcaaccaaac gttctccctg atgtaattca acccacatca
    10201 ctggaaatac actaccagtg aggttccaca gccatgggtg cgataaatgt gttctgccta
    10261 gacgagggat gattgtgaca ttatatcttt tttgttctct tacaaatggg atgtcaccag
    10321 aatatctgcg ggattgtatt aatgatttgt aaaaatatga ttccttttga acgctttcat
    10381 atccaccggt ttcagaagca aagacttccg catcaaattg aacgatgaga tttgctggta
    10441 tcgttacatc atgactatgt cctgggacgt acgaatgcct tccaaaatta atcgaaacat
    10501 aatccacctc ggttccaagg aaatttacat actccttttc tgcgactttc aattggatgc
    10561 cgtcacgttc gtcagtacct gatgttacta caccttgatt ggaggaaaag agaaccgcat
    10621 ttgcgatacc tttttttccg tatccataac ataaccatgc ttctgaaccg agagttgcgt
    10681 catttttgta ggatatgatc cattctcgtt ctttcgtgga aggattttga tttaattgat
    10741 attgttcaac cttatctttc tcgctataga aattaaggta cggatagatg tttccaaagt
    10801 ttaatgaatc aacagcagca cttcgtgatt ttaaggaaat cagagaacca aatcttccat
    10861 ctatattatc aataccttgc accgtagcat ctttcaacat ctcatgtttc acatgaacat
    10921 ttatacgttt ttcttcaatg ggtgagtaat agtatctata aattgctgtc gtctggacgt
    10981 ccttcttttt cgactcacta aatattccaa actcgaccat gagattgcca tcgatgaaaa
    11041 tttttttatc gagaaacacc tgatcggatg aactttcatc tttttcttta tccccataat
    11101 aatatgaaaa cgcaaatgat acgatttgat cggaatcaat gatatcgaat tccttacatt
    11161 ctttcttttg ttttaccact acctgagcac aggatctatc taaaagctgt ccctcttgtc
    11221 ctactccata gatgcaatat ccgtcttctg tgattccata gaatcttgct acggcggata
    11281 tctcaggtaa cggggatgcg gaataattca gatcttcaat ggtcacatgg tcgatataat
    11341 cgggtcttgg tgttttctca tcatcgtaat acatgaaata ctgttctgtt ccatctgcaa
    11401 aagcggggac caaaaaaatc acattacatt cgccgatgaa gcttgtttcc cctgggattt
    11461 tttcaagaga atagatttgt gattcgagat cataccatat gtctgcatgc cagcaggcaa
    11521 tccgaataga cgtattattt tcattttctg tccagcaagg gttttcgaac cgtagacgga
    11581 ggtcgattgg ctgataatgg gagacgtttt catctgtttg aatgggaagc ggaatctctt
    11641 gcctatagga ccattcttta tagtctgtat cccacgatgg tccgccgtca ccttgcgacg
    11701 atcctggtaa acttaccgct ggtatgacaa gcagaagaag tatcccaact atgactatgt
    11761 ttgaccgtcg cataggtctc cattctttgt atttatttta ctgttctata taaaagagtt
    11821 gttttatgtg tataagaacc tttttaatat ggagaaatgc ctatttctcc ttatctatta
    11881 taaaagtgaa gaaatgacgc ataacggtca attagataac agtgaaaaaa ttcagttgcc
    11941 ctaattttcc tagaataata agggctgtaa atccaatcgc ataaaaaaga taggttatat
    12001 acatcaatga ccaagctgaa acgttttcta aggtcccact ttgttgttgt ttcagcatta
    12061 agtaaaagat aacgaaacca gggataatga ataaggtaag aggtaaaaag tatgggtcta
    12121 tcaattggta atgaataagc actggaaaca ttgcgaatgg gatgatgagg aagggaagtg
    12181 atatgaatgc tgcttttctc gttccatacg tgttgataag agtccgtgtc cctgtttttc
    12241 tatcagcttc acaatcaacg atatctttcg tggtcattgc tccgatgaaa tacgtaagtc
    12301 caataaggcc aattattaag ggtactatct cgaacgggtc accgaagacg ctccatgagg
    12361 caagaattgc aaataatcct cgcgggatgg caatccatac ctgattaata aacaggaatc
    12421 gtttcatccg tggtgggagg gaatagatga cggtaaacaa catgataagg aaaacaaaaa
    12481 ctccgaacca ggtattgatt gttactgcac gtaacagggc gaagagatac agaatgtatg
    12541 caatgctttg tgcttcatcg cgctcgacga caccacgggg aattgggcgg taaggttttg
    12601 atattttatc tgcctctaca tctgttgctt gattcaatgc attcgaagca ccattgagaa
    12661 acgctaaggt aaggcttgct tggccgatga cgatccacca gttagggaat tctacgaact
    12721 tgtcattgta gacgagactt gcgaacatga taaacattga gacaaaaaaa ggggctagta
    12781 acgtaaaggg tctgagtaaa tcgaaatatc ctctaaattt ctttgaaaga aacctacctt
    12841 ttgtatatgg ttctttcatc gttaatttca cagacattat ggcaccaaga acatcggtaa
    12901 atcgttgttg tgaatacaga tggagattat attaattact ctaaggggcc aagatatctt
    12961 tttagagaat cgcttccgtg aaaaccgtat gacgttagac agatttactt agcatttctt
    13021 ggatgattcg tgcagaattt atcgcacaat taattccaat gcctggtgct ttcacacagt
    13081 caccaataat gtaaaggttt tcgatgggtg tctgtatgtc tggcttcgtg tttactaggg
    13141 tttttgcgac accatcgaga tgccagactg aggggtaaat agcccattgt aattgtgaat
    13201 gatattgagg gatgatgcgt tcgaggtttt tatctaaagc ctctttcagt ttgttaagaa
    13261 tttttttatc cttggcttct tttggcgtgc aaataatata tgattgaacg agataatggg
    13321 acgaaggtga taaccctgtt tcttccattg cagtaataaa atcgatcata ccgacagcat
    13381 catttccgtc aacaccgatg tcccgctcaa tgaaaataaa agattttccc aataaccggg
    13441 ggtcaatctg atttagggaa taatacgcgc aaagactccc ggtcccctca agagtcttca
    13501 ttgtggtgac gtattccttt ggaaactgat gttcatcagc aatggtaaat aggttttgga
    13561 ccaacatgtt ggataggacg atatcgaaca tatattcttt gtccgcgaca acaacgcctt
    13621 cagctttctt gttcttgata agaattttcg ataccgggga tttcagatgg atttttccac
    13681 cgtttttttc gatatactcg gcaattgttt gggatatttt taggagtcca tgcttagggt
    13741 agtttacgcc ccctagtttc atgtcttttt gtgttctgaa cacttcacct gtcgatatca
    13801 aatcaagatt atttatcgtg cttattaaac gcggattcac ctcaagtacg atcttcattt
    13861 tcccttttcc atattttttt atcgtctcgg tcatggaaac agcatccagg tctttcattg
    13921 tcttttcacc cgaaagaaga agtcttacga tattgggcaa catcgatatt ttatcagccg
    13981 tggtcacaaa atagccgaaa tgattgtttt tctgttcata taaccgggat ttatacatgg
    14041 tgatattatc aaaatactcc ttaaggatat ctcgaagttt gacgacgatt ccgcttccga
    14101 tcatatggaa ccctaaatca agagtgtagt tctcgaacat cttattttta aagatttcct
    14161 ccaggggggg ttccgaaaaa acgattgctg tattgaattt ggccaaggtc tgtttatagg
    14221 attctaaggt ttgggatgac atttctaagg ataacgctct tcctccgacg attggttcct
    14281 tttcaaagat atcaacggaa tatccttttt ttagaagaag tgcaccggct gttaaaccac
    14341 cgatgccagc accaatgatg cacacttttt ttttattcat gttcaataac cccttttttg
    14401 tttattttaa cagatgcaat cggtttttca atgagctata aaaaaatctt cgtactatct
    14461 tccatttata ttgctgtaca ctcaattgtc cggttatgac tcccatcatc agatctgtca
    14521 gcactggatc tttgtaggct attttgaaaa ctttttctac gtattccatg cttcctcgtt
    14581 gcacaacctt taatatcaga ttgatatctt ttccaaaatc gttttgccag cgggtttgat
    14641 attgtgataa gaactcctgt gttgtctgtt ttttctccaa ggcttctgtt attatctctg
    14701 cagcgatttg tcctgatacc attgcgtaat aaattccttc accggttaag ggattgataa
    14761 atccggcggc atctccaacc agaaggaacc ggtctgcata ggttttttcc aacggatgcg
    14821 tcagtaacgc ccctcctttc accggggttt cttttaggtg ttgtggtata agattttttt
    14881 ctttcaataa tgtgatatag tcttgatacc agttgaggag attgtgtttc ggctgctggt
    14941 ttttatccat attaatgatg ccaaatccaa tgtttaaatg ctcttttttc ggaaacaccc
    15001 atccgtaccc agaggctgat ttgaaccttg aatggatata acaatggcga gattttttaa
    15061 aatactcatc catgattttt tcgtctacct cgaattcttg caggatacag attccttttt
    15121 ctgtccgact tgctcgcagt cccatgtttc gtacaagtgt gctgtttact ccatcagcac
    15181 caacgattat ttctgaatct atttttcttc ctaggtctaa tgtcacctgt gctgtgtcgt
    15241 tggagattcg gacagcggat gcaggtgtcg cctcttggaa aatagcacct gcttcaatgg
    15301 cgaatttcac caattcaaaa tcgaattttt ttcttagaac catcgctatg atgggtgtat
    15361 ttttgttgat ttcaatctga tgttgtaaag atggggaaaa gatcgtccca ctcgacgaat
    15421 atgcttcgat gatattatca ttgatgacat aggggaatct ttggagcaca cgtatgggta
    15481 atcctccacc gcatggtttc tcgcgtggga atttttcttt atcgagtaaa agagttttaa
    15541 aacctttttc agctagaaat ttcgcggttg ttgaccctgc aggtcctgct ccgactatcg
    15601 tgacgtcgta tttcatagat tccccaaatc atgaaatcct gtcttcattt ctatacattc
    15661 ttggtttgaa tatatgatga agataaggaa aagcggatag aaatcagtta ttcccatcgt
    15721 taacgcagcg atgacaggaa caattccaag ggcaccaata ccagggacac gaaggagttt
    15781 tgcgtacgca gcacccttgc tcatggaagt gtgt
//
LOCUS       DATMXJ010000073         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_27279, whole genome shotgun sequence.
ACCESSION   DATMXJ010000073 DATMXJ010000000
VERSION     DATMXJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27279"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..>5383
                     /locus_tag="VN377_02180"
     CDS             546..>5383
                     /locus_tag="VN377_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR27224.1"
                     /translation="MPATMLSKYRLHPVFFVNRTIHINQSTSINLWDWEKNDSSLQAK
                     FNITERKNENIESYNVICEVPGKNQSKSIMISGGHHDYFPGQGAADNTVGVATMLGIL
                     RWLNESNITPEYNITFVSWAGEERIFRGSSSYVFNQSNYCKNENITYMINLDFFAYDS
                     PGSILNIITSTNAFCNSVSNITNRTDYSKRTQYGIRVTEEDGKHYKIADGAKPLYLCY
                     VDDSPLNKKLGPFKKKTDLQIVTVGKPNMRDIVRHRSGGNFEFGDVLNHINQTDLNCT
                     AEMVLNITKYLVLEPPENVFVNCSYTPFDLCGDGWNDSVNISFNVTTNLTSWATAKAC
                     LYNITTGQPVSDVNETSFTIYKGTNTSGHLAVTLYPIIANGTYNASIRIYDDRMNLDD
                     ECHQLINLSPYGKPIANYDYELSGLINHSVDFTDTSMASPGADIDEWYWNFDDGTHSH
                     EQNPSHLYLLSKDYTVTLTVWDTNDFNDSWSDTLTIPNSSPYTSFTISDYAVNVETEL
                     SFTSTSTDEDGTIENYTWDFGDQSVSYEENPDHSYTQSGVYTVTLTTIDDDDATNMTT
                     KSDCLIIANALVDDDFHDDPDAHEWDTIQEGINDVDNGGIIFVYNGSYDAIEINKPVA
                     LYGESRQGTFISGGDTVVQITDRNVSVNGFTVNGGTNGFDVYVKYDENHSSNVTIKNC
                     DISENSNIGVLLNQSNDCLIENCSFTKNTYGVKIINNSEHNIIKKSVFSYCTYGVYVA
                     NSSSNFIGSPSISSPYPTDCLFTYNDTAMYLKNAEYNFILGCDIDGAPNPGIASTKGI
                     CLDDAENNTISTCYIHDVTNTGVYLHDSTWNKIEHSKIIKNPNGITFANSPENLIVQN
                     HFGINTEYAIYLPSNSQNNHIYYNDFFVNGNSSTNQSWDANDERGKENLWSKEGNNTL
                     TKTGPGEGNYWSDYTGGDEDQDGIGDTPYLLNSSGAERNDSYPVMEPYGWCDFTQDST
                     PPEFSNVSSTPSTVGFGSNVTISASITDNSSNISIVKVNIIYQNQSIGNYTMDDVGGS
                     TYQYTFTDTWTVGQYNYTLWAMDETYNTNSSSEYHFHISANATISIATLKDNYSGNEY
                     INITDPPNPTENLTLVGRGLTWDEYYNAITGQNILEVSTGPVNYQENNGTWTPINNTL
                     NQITSTHPAYNYGYRTGNDRGLFGVYFKPNIQSDWPIAFTYNRSDNPTTSVIRSKLIG
                     VGYVDPSSNWTYQYLQNVQSSQAQTNGNAITYEDVFTGTDVTWSYENTELKEAITMNN
                     ATKTALQNHPPSLYGLNNASSYLVFITKLDHQNLNLYNASELLNGNVTICDQGVDFKD
                     TLGRFKCALPLGEAYELNNESMREKLTYRIVHLNGNTYLLSGLKLSDLNSMMFPVVID
                     PTLTVYSSSSDGYRSYQSTNYQSCWNATTGNGFYGAYNIFIGQNKQSTTYTIYRGYVY
                     FNTSSLPSNAYIDSATLGLYKSSDYSTTNFLITVQNGQPTYPHDPLQTADYNRAYYSG
                     DGGTLNTDSFGNGYNNITLNSDGLSWINRTGWTKLCLRSSRDINGNTPTGNEYVSVYT
                     SEQGSSYTPKLVIAYRNQSKIKNNGSTDIKGYLLIQVQFYDDEAEPGAWIVENDTVNE
                     TSP"
BASE COUNT         1727 a         1293 c         1068 g         1295 t
ORIGIN      
        1 tctaaaacag ttgtatctgt tgtgggtgac gaacatcatg gtcacccagt tattgcgagt
       61 atctcccgca atctttttag ggctacagct aattacattt tccatacaaa tattttctcc
      121 tgtcgcacct ttagagggca gacgaatgcc tgccgcgtgc gacaaaaaag agatatactt
      181 tagctggaga aaaatagaga tgcgactttt catctgcggg tctttcactt cgtttcagac
      241 cacgcagtat tcccagtcgc aactataaca atgcaacctt aattttatca ttaccggaaa
      301 ctgaatatga ttcttcacaa aacattggaa tttgttatct ttacgttttt aaccaacgtt
      361 tcgaagagaa aaaaatcgga ttattcgaag aaacaagcga ctatcccccc attagtttaa
      421 atatattttg caatattccg tttccttgtt caccctatgt tggttttatc ttatgtaata
      481 aaacaatttt tgataacact cattacatgc ttacagcaac gggtatacct tatgaatctc
      541 aaaatatgcc agcgacgatg ttgtcgaaat ataggttgca tcctgttttc tttgttaaca
      601 gaacaattca tataaatcaa agtacttcaa tcaatctctg ggactgggaa aaaaatgatt
      661 caagtcttca ggcaaaattc aatatcactg aaaggaagaa tgagaacatc gagagttata
      721 atgtcatctg tgaagttccc gggaaaaacc agagcaagtc aatcatgatt agcggtggtc
      781 atcatgatta tttcccgggt cagggggcag cagataacac agtgggtgtc gcaaccatgc
      841 ttggaatatt gcgatggcta aatgaaagta atatcacccc ggaatataat atcacctttg
      901 tttcatgggc aggggaagaa cgcatctttc gtggatcatc cagttatgta ttcaatcaat
      961 caaattactg caaaaatgaa aatattacct atatgattaa cctggatttt ttcgcctatg
     1021 attcacctgg ctctatattg aatataatta cttctaccaa tgcattttgt aatagtgttt
     1081 caaatattac aaatcgtact gactattcaa aaagaacaca atatggaata agagtaactg
     1141 aggaagatgg gaaacactat aaaatagctg atggcgcaaa accgttatat ctctgttatg
     1201 ttgatgattc tccactgaac aaaaaattag gaccgtttaa aaagaaaact gatcttcaga
     1261 ttgtgactgt cggaaagccg aatatgcgag atatcgttcg gcataggagt ggtggaaatt
     1321 ttgaattcgg cgatgtgttg aatcatatta atcagacaga tttgaattgt acagccgaga
     1381 tggtgcttaa tataacaaaa tatctggttt tagagccacc tgaaaacgtg tttgtgaatt
     1441 gtagttacac cccctttgac ctgtgtggtg acggttggaa tgattccgta aacatctcgt
     1501 tcaatgttac cactaactta acgtcatggg cgacagccaa agcatgtttg tacaacatta
     1561 caactggaca accagtgtcc gacgtaaacg agacaagttt cacgatctat aaaggtacga
     1621 atacaagcgg tcacctcgcc gtcacccttt atcccataat agccaatggc acatacaacg
     1681 cttccatacg tatctacgat gacagaatga atcttgacga tgaatgccat caactcatca
     1741 atctctctcc atatggaaaa ccgatagcaa attatgatta tgaacttagt ggtctcataa
     1801 atcatagcgt tgatttcacc gacacatcaa tggcatcacc aggagcagat atcgatgagt
     1861 ggtactggaa tttcgacgac ggcacacatt cacacgaaca gaacccatct cacctctacc
     1921 tcttaagcaa agactatacc gtgacgctca ccgtctggga caccaacgac tttaacgata
     1981 gctggtcaga caccctcacc atcccaaata gctcaccata cacgtcgttt acaataagtg
     2041 actatgcagt gaacgtcgaa acagaactca gctttacttc aacatccact gacgaggacg
     2101 gcaccataga aaactacacc tgggattttg gtgatcagag cgtatcctat gaggaaaacc
     2161 cagatcactc ctacacccaa agtggagtct acaccgtcac cctcaccacc atcgatgatg
     2221 acgacgcaac aaacatgaca acgaaatcag actgcctcat tatcgctaac gccctggtcg
     2281 atgatgattt ccatgacgac ccggatgctc atgaatggga cacgatccag gagggcatca
     2341 acgacgttga caacggcggc atcatctttg tatacaacgg cagctacgac gcaattgaaa
     2401 tcaacaaacc cgtggcattg tacggagaaa gcagacaagg cacgttcatc agcggaggag
     2461 acactgttgt acaaatcaca gatagaaacg tttccgtcaa cgggtttacc gtcaacggtg
     2521 gaacaaacgg atttgatgtc tacgtgaaat atgatgaaaa ccattcaagc aatgtcacca
     2581 tcaaaaactg tgacatctca gagaacagta acatcggagt ccttctgaat caatcaaatg
     2641 attgtctgat tgagaactgc tcttttacaa aaaatactta tggggtgaaa atcatcaaca
     2701 actccgaaca caacatcata aagaaaagcg tgttctctta ttgcacgtat ggagtatacg
     2761 tcgcaaactc ctcatctaac tttatcggat caccatccat ttccagccca tatccgactg
     2821 attgcctgtt cacctacaac gataccgcga tgtacctgaa gaacgcggag tacaacttca
     2881 tcctcgggtg tgacattgac ggggccccta acccggggat agcatcaacg aaaggaatct
     2941 gtcttgatga cgcagagaac aataccatct ccacgtgtta tatccatgat gtgacaaaca
     3001 caggtgttta tctccatgac tcgacctgga acaagataga gcatagtaaa atcatcaaaa
     3061 atccgaacgg tattaccttt gccaattcac ctgagaatct gattgttcag aatcactttg
     3121 ggataaacac tgaatacgcg atctaccttc catcaaattc acaaaataac catatctact
     3181 acaacgattt cttcgtcaat ggaaacagct caacaaatca atcatgggat gctaacgacg
     3241 aacgaggaaa ggaaaatctc tggagcaaag aagggaacaa caccctcacc aagactggtc
     3301 ctggtgaggg gaattactgg agtgattaca cgggaggaga tgaagaccag gatgggattg
     3361 gcgatacgcc gtatctgctc aattcaagtg gtgcagagag aaatgatagt tatccggtca
     3421 tggagcccta tggttggtgt gattttactc aggattccac accgcctgaa ttctcaaatg
     3481 tctctagcac accgagtaca gttggctttg ggtctaacgt gaccatctct gcttcgatca
     3541 ctgacaacag cagcaatatc agtatcgtga aagtgaacat catctaccag aaccaatcca
     3601 ttgggaatta caccatggat gatgttggcg gctccacata ccaatatacg ttcacagaca
     3661 cctggacagt cggacagtac aactacacgc tctgggcgat ggatgaaacc tataacacca
     3721 acagtagcag cgaatatcat ttccacatct ccgcaaacgc aaccatcagc atcgccaccc
     3781 tcaaagacaa ctatagtggc aatgaataca tcaacatcac agacccacca aacccaacag
     3841 agaatctcac attagttggt cggggtctca cctgggatga atactacaac gcgatcacag
     3901 gacagaacat cctggaagtc tccacaggac cagtgaacta ccaagagaat aacggcacat
     3961 ggacaccaat caacaacacc ctcaaccaaa tcaccagcac tcatcctgcc tacaactacg
     4021 gataccggac agggaacgac cgcggactct tcggcgttta cttcaaaccc aatatacaaa
     4081 gtgattggcc aattgcattc acgtataaca gatcagataa ccccaccact agtgtcatca
     4141 gaagtaaact tataggtgtc ggctacgttg acccatcaag taactggacg taccaatact
     4201 tacagaacgt gcagagtagc caggctcaaa ccaacggcaa cgccataacg tatgaggatg
     4261 tctttaccgg cacagatgtc acctggagct acgagaacac ggagctgaaa gaagccatca
     4321 ccatgaacaa cgcgacaaag actgctctac agaatcatcc accatcattg tatggtttga
     4381 ataatgccag ctcatatctt gtcttcatca ccaaactgga tcatcagaac ctcaacttat
     4441 acaatgcatc agagctgctc aatggaaacg tcactatctg tgaccaaggt gttgacttca
     4501 aagacacact tggtcgattc aaatgcgctc tgccacttgg cgaagcctat gagctaaaca
     4561 acgaatcaat gagggagaaa ctaacctacc ggattgtgca tctaaacggc aacacctacc
     4621 tgctctccgg actcaaactg tctgacctaa atagtatgat gttccctgtc gtcatcgacc
     4681 cgaccctaac agtctattcc agctccagcg atgggtaccg gtcataccaa agcacaaatt
     4741 atcaaagctg ctggaacgct acaacaggca acggtttcta cggtgcatat aatatattca
     4801 tcgggcagaa caaacagagc acgacgtata ccatctaccg cgggtacgtc tacttcaaca
     4861 cctcatcatt accgtcgaac gcctacattg actctgcgac actgggcctt tacaaatcta
     4921 gtgattattc gaccactaat tttctaataa ccgtccagaa tggacaacca acctatccac
     4981 acgacccact acaaaccgct gattataaca gagcctatta ttccggggat ggaggcaccc
     5041 tgaacaccga cagcttcgga aacgggtaca acaacatcac attgaacagc gacggcctca
     5101 gttggattaa ccgtaccggc tggactaagc tgtgtctgcg gagcagccgg gacatcaatg
     5161 gcaacacacc aaccggaaat gaatacgtca gcgtctacac tagcgaacaa ggcagcagct
     5221 acaccccgaa gctggtgatc gcctaccgga accagtcgaa gatcaagaac aacggatcta
     5281 cagacatcaa aggatatctg ttgatccagg tgcagttcta tgatgatgag gctgagccag
     5341 gggcatggat tgtcgagaac gatacagtga atgagacatc gcc
//
LOCUS       DATMXJ010000074         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_59146, whole genome shotgun sequence.
ACCESSION   DATMXJ010000074 DATMXJ010000000
VERSION     DATMXJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59146"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VN377_02185"
     CDS             <1..559
                     /locus_tag="VN377_02185"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWR27225.1"
                     /translation="LYMTLDKKIEGLLLQATELGINFEPAIQTGQVKFLFLNINKKLI
                     YESMINEILSGNYDRVVLDSITPLSEMPIYVNNPDFATEDNTIDHEEITSGNFPVRRL
                     HLHYIMNALETAKCTAVVTSELPSGSTNYSRDGLSEFLADGVIILSLDPTMDRRKLAV
                     MKMRSTKHTLKPQNIEIGQGGMKII"
     gene            565..1032
                     /locus_tag="VN377_02190"
     CDS             565..1032
                     /locus_tag="VN377_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWR27226.1"
                     /translation="MFHYRPPKELRALFIVHYAPLLFIILIGPLTAYYGVWEFPLPKL
                     LTIILGVAIFLLGAYVYFKWEIFWHKTYKGQLVTGGIFQYIRHPHYTSVLIVGFGLAL
                     FFYSVAAFIIALIAIPIMIWSILDEEKLLTKQYGEPYKEYMKKVPWRMIPKFF"
     gene            complement(1040..2134)
                     /locus_tag="VN377_02195"
     CDS             complement(1040..2134)
                     /locus_tag="VN377_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HWR27227.1"
                     /translation="MGEAEKMQKSAFYVSLSKDGVTTKQESDNISEIVGHLREDALEW
                     MDFTVENINEDSFVIATSLKFSLQLVASVVADRYSSYEDLDTELGLMLPAVRVDQFDV
                     NVHPLLIFIRKNLILTIHPKDVVRLQKIYRYADIFMKKIRQDLPWNDKLTILLTRIID
                     ENNQKNFNGLRMIEEEGDELGKYMVDISLPRVEIGNDIYKMKHALITYLNTLWASWDV
                     INSLRYGDAETITDNQKLLQRVGILGDDINRQIALSEHMSEVLASGLEVLQSVYNNQL
                     QILNNRLALMVGYLTIIGTALLVPNTIATIAGNQMFPFTTMDIPAYLALLIGSTIIAT
                     IVSWWTVKKYGLLPKRPDENDAPKKNSRKM"
     gene            2308..3747
                     /locus_tag="VN377_02200"
     CDS             2308..3747
                     /locus_tag="VN377_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27228.1"
                     /translation="MKVLFIEPPSSTSTSMLPPISLPTLKGFIKTKTTHNAKILDLLF
                     HGKDWQQRVLEEIKKEKPDLIGFSMLSFNYQAILGIARFIKQHSTIPIIAGGVHVILS
                     PQEVLSNQEIDIVCIGEGEYVLKELLDNSLNRSGVDGIWYKEKGEIMKNKPRQLITDL
                     DELAFPDFDDFDIQKCLFIYNNHLSIMASRGCPYSCSYCNNHALRRTLKGTYVRFRSV
                     ENVIEEIDVRITQYYDKGFRYFYFFDDTFILNKDYVLKFCKQFKEKGFHKLLRWNVNI
                     RANLVTDELMKALKDAGCYQVRMGIESGNEFIRNTIYHRGMANQEIYDAVDIIKKNNL
                     QLRLYFMVGAPQETIEMMHESLRMAQSLNADEIFFGVLYPLPGTDIRKLCEDEHVLET
                     KNGSGFSEIAAIKKTKYVSSAQIRRFLRQVQRWQMRQYIQVGLELRGPLFILDCLRFI
                     FLYKRKYHFEMDQLFRWNVQRYTLAQIWN"
BASE COUNT         1213 a          864 c          801 g         1149 t
ORIGIN      
        1 cctttacatg acactcgata agaaaatcga aggtctcctc ctccaagcaa cagagcttgg
       61 gataaatttt gaaccagcaa tccaaaccgg acaagtcaaa tttctctttc tcaacatcaa
      121 caaaaaactc atctatgaaa gcatgatcaa cgaaattctc tctggaaatt acgaccgagt
      181 cgtcctggac tctatcaccc ccttatcaga gatgcctatc tatgtgaaca atcctgattt
      241 tgccacggag gacaacacga ttgatcatga agaaatcaca tctgggaatt tccctgtccg
      301 acgattacat ctccattaca tcatgaacgc gttagaaaca gctaaatgta ccgctgtggt
      361 aacctcagaa ttaccatctg gctctacgaa ttactcacgt gacggtcttt cagaattcct
      421 tgcagatggc gtcatcatcc ttagtcttga cccaacgatg gaccgacgga agcttgccgt
      481 gatgaaaatg agaagcacca aacacacact caaaccacag aacatcgaaa ttggacaagg
      541 cggtatgaaa ataatctaag agctatgttc cattacagac caccgaaaga acttcgagcg
      601 ctttttattg tacactacgc accattgctg tttatcatcc tgattggacc gctgaccgca
      661 tattatggtg tctgggaatt ccctttacca aagcttctca ccatcatact aggtgtcgct
      721 atctttctcc tcggggcgta tgtgtatttc aaatgggaga tcttctggca taagacatac
      781 aaagggcagc tggtgactgg ggggatattc caatacattc gacacccgca ttacacctct
      841 gtgctaatcg ttggatttgg tcttgcgttg tttttctatt cggtagccgc ctttatcatc
      901 gctctcatcg caatcccgat catgatctgg agtatccttg atgaagaaaa actcttaaca
      961 aaacagtatg gcgaacccta caaagagtat atgaaaaagg tgccttggag aatgatacca
     1021 aagttttttt aatctctctt cacatttttc tactattctt ctttggcgca tcattttcgt
     1081 caggtctttt cggtaacaga ccatatttct taacagtcca ccaggagaca atagtagcga
     1141 tgatcgttga tccaataaga agggcgagat aggctggtat atccattgtt gtgaaaggaa
     1201 acatttgatt ccctgcgatt gttgcaatcg tgtttggtac aagaagcgca gttccaatga
     1261 ttgtcaggta tccgaccata agcgcaagcc ggttgttgag gatctgtagt tgattattat
     1321 aaacactttg caggacctca agtcctgagg caagcacctc gctcatatgt tcgctgaggg
     1381 ctatctgtct gttgatgtcg tctcccaaga ttccgacacg ctggaggagt ttctgattat
     1441 cagttatagt ctctgcatcc ccatatcgca acgagttaat cacatcccaa gacgcccaca
     1501 gggtgttcag ataggtgatg agagcatgct tcattttata gatgtcattg ccgatttcca
     1561 cccggggtaa acttatgtcc accatgtatt ttccaagctc atcgccttct tcctcgatca
     1621 tccgcagtcc gttgaaattc ttctgattat tctcatcaat aatcctggtc agcagaatcg
     1681 taagcttatc gttccagggg agatcctgac ggattttctt catgaaaata tctgcgtatc
     1741 ggtaaatctt ttgtaagcga acgacatctt tggggtgaat cgtcaatatc aaattctttc
     1801 gaatgaaaat aagcaaagga tgcacattga catcaaactg atcaacccgt actgcaggaa
     1861 gcattaaccc aagttctgtg tctaaatcct catacgatga atatcgatcg gctacaacgc
     1921 ttgcgacaag ctgcagacta aatttcagag aagtcgcgat aacaaaacta tcctcattga
     1981 tattttcaac agtaaaatcc atccactcga gagcgtcttc ccgtaaatgt ccgacaattt
     2041 cggagatatt atcactttct tgttttgtcg tgacgccatc tttcgagagt gatacgtaaa
     2101 aagcgctttt ttgcatcttt tcagcttcac ccatacgtaa ctctccctag atttagaact
     2161 caagtaaatt tgactctaca tccgcaagga acatataaag gtacgtgttt gttgtcgaac
     2221 acatagagga aaccttcatc attttcaaag actataacat ccaatttata acttcatgat
     2281 cgttatggaa accatgggga ggaacctatg aaggtcctgt tcattgaacc accaagctca
     2341 acatcaacat ctatgctccc gccaatttct ttaccaacat taaagggatt tattaaaacc
     2401 aaaacaaccc ataacgcgaa aatccttgat ctattgttcc atggaaagga ttggcaacag
     2461 cgcgttcttg aggaaataaa aaaagaaaaa ccagatctca tcggtttttc aatgctcagt
     2521 tttaattatc aagcaatact agggatcgca cggttcatca aacaacacag taccatcccg
     2581 attatcgctg gaggggttca tgtcatcctc tccccccaag aagtcctatc gaatcaggaa
     2641 atcgacatcg tatgcatcgg cgaaggagaa tacgtgctca aagaactcct cgataattcc
     2701 ttaaatcgtt caggagtgga cggcatttgg tataaagaga agggagaaat catgaaaaat
     2761 aagccacggc agttaatcac cgatttagat gaattggctt tccctgattt tgatgatttt
     2821 gatatacaaa aatgtctgtt catctacaat aaccatttgt caatcatggc atcgcgaggc
     2881 tgcccgtatt cgtgttcata ttgcaataat cacgcattac ggaggactct gaagggaacc
     2941 tacgtgcgat tccggagtgt ggaaaatgtc atagaggaaa tcgatgtacg tattacacaa
     3001 tattatgaca agggattccg atatttttat ttctttgatg acacctttat tttgaacaaa
     3061 gactatgtcc tgaagttctg caaacaattc aaagaaaaag gatttcataa gttgcttcga
     3121 tggaatgtca atatacgagc gaatctggtt actgatgagc tcatgaaggc attgaaagat
     3181 gcagggtgct accaggtacg catgggtatc gaatctggaa atgagtttat tcgaaataca
     3241 atctaccacc gggggatggc caatcaagag atatacgatg cagttgatat tatcaagaaa
     3301 aacaatctgc aactccggct gtatttcatg gtcggagcac cacaagaaac cattgagatg
     3361 atgcatgaaa gcttgaggat ggcacagtca ctgaatgctg atgaaatctt ctttggagta
     3421 ctgtatccat tgccaggtac ggatatccgt aaactttgtg aagacgaaca cgtccttgag
     3481 acaaaaaacg gatcaggatt ttctgagata gcagcgataa aaaaaacaaa gtacgtctcc
     3541 tcggctcaga tccgacggtt cctccgtcag gtgcagcgct ggcagatgag acagtatatt
     3601 caggttggtt tggaactacg tggaccactt ttcattctgg attgcctgcg ttttatcttt
     3661 ttatacaaaa gaaagtatca ttttgaaatg gatcagttgt tccgttggaa tgttcagagg
     3721 tatacactcg cccagatttg gaattgatgg acgaacgttt ttattgggcg ctagatatca
     3781 gattttcaat ccaatagaca ttacatatgt agttttattg taattgtagc attatggctt
     3841 ttcagcacaa taaatattta taatatcaat cctaaagtat aacatgataa tctttatcac
     3901 tgaaggtggt ttctgatgaa taaccctaaa attgaaaaaa aatcgacagg agacgaaaaa
     3961 cctggacgga tactgattgt tgatgacgat ccagatttca gagatttgct caatctcatt
     4021 ctatgta
//
LOCUS       DATMXJ010000075         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53836, whole genome shotgun sequence.
ACCESSION   DATMXJ010000075 DATMXJ010000000
VERSION     DATMXJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53836"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN377_02205"
     CDS             <1..180
                     /locus_tag="VN377_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27229.1"
                     /translation="SLDLMKLQLHKMVNQMTKEKRHMIFSEVKEENIKKNIQGHAMVE
                     DMGHHGKGNGHGRPS"
     gene            207..413
                     /locus_tag="VN377_02210"
     CDS             207..413
                     /locus_tag="VN377_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27230.1"
                     /translation="MNKKIISLLSLLILLVSVTVSYAYFSQPIPSDNQQTNTDGDITD
                     STMTSEIDSTFLNETQGIEIGDMI"
     gene            519..1388
                     /locus_tag="VN377_02215"
     CDS             519..1388
                     /locus_tag="VN377_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWR27231.1"
                     /translation="MERVVKNIFKSRLTLEGAGVRLHRVFGYHQVPLFDPFLLLDHFG
                     SRDPEDYVKGFPWHPHRGIETVTYMLSGQVEHGDSLGNTGVIDSGDLQWMTAGSGIIH
                     QELPQRYDGLLEGFQLWVNLPAMKKMTTPRYRGIIASQIPIVHHEDATLKVIAGKVAG
                     TQGPVTDLMVDIEYYDVTLAKGKDFIRPTKKGYTTFLYVISGSGSIVDKKIIPHHGIL
                     LSDGDQVRISAEDMLRFLFITGEPLKEPVAWGGPIVMNTEAELGQAFKELDEGTFVKT
                     SVPASLSTGFYRQ"
     gene            complement(1410..2237)
                     /locus_tag="VN377_02220"
     CDS             complement(1410..2237)
                     /locus_tag="VN377_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWR27232.1"
                     /translation="MEEKKIKKKVREGYAKIAKQETSCCGSIDVCCGTANSPVEISKT
                     IGYTDEQLQSVPDGANLGLGCGNPTAIASLKKGEIVLDLGSGAGFDCFLAANKVGPTG
                     KVIGVDMTPEMLKKAKENAKKSGYTNVEFRRGEIEDLPLEADSVDVIISNCVINLSPD
                     KKKVFQEAFRVLKPNGRLAVSDIVLLRELPEKIKNSVEAYVGCISGAILKDRYLQLLK
                     DAGFIDVTVTDETSYQVDVVINKPRTQAVIEEAGITMEEFKKIGGSFVSIKVTGKKP"
     gene            complement(2257..>2442)
                     /locus_tag="VN377_02225"
     CDS             complement(2257..>2442)
                     /locus_tag="VN377_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HWR27233.1"
                     /translation="ERGQMVLPKELREKANIRAGDKLAVVAMEKDGKICCLSLIKVDD
                     MEPMVKGILGPIMKDIL"
BASE COUNT          627 a          543 c          530 g          742 t
ORIGIN      
        1 agcttagact tgatgaaact gcagctccac aagatggtca atcagatgac aaaagaaaaa
       61 cggcacatga ttttctcaga ggtcaaagag gagaacatta agaaaaatat acaaggacat
      121 gcaatggttg aagacatggg acatcatggc aaaggaaatg gtcatggaag accaagttag
      181 gatgaaatga tcagggatga tagaaaatga ataaaaaaat aatctctctc ctttctctct
      241 taattctttt agtatcagtc accgtctcct atgcgtattt cagtcaacca ataccaagcg
      301 ataatcaaca aaccaatacg gatggcgaca ttactgatag caccatgaca tccgagatcg
      361 attcaacgtt tctgaatgag acccaaggga tagaaatcgg agatatgatt tagatagtaa
      421 cgattaccat ccttccttct ttttttttta tttattacga tagaaactta ccatattgag
      481 acaaaaattt tattagccgc tttgtgtatt gaaacatcat ggaacgggtt gttaaaaata
      541 tttttaaaag cagactaaca cttgagggtg ctggtgtacg acttcatcga gtttttgggt
      601 atcaccaggt tccattgttc gatccatttc tcctccttga tcattttgga tcgagagatc
      661 ctgaggatta tgtcaaaggg tttccctggc atcctcatcg ggggattgag accgtgacct
      721 acatgctttc cggtcaggta gaacatggtg atagcctcgg gaatacgggt gtcatcgact
      781 caggggatct tcaatggatg accgctggct caggaatcat ccatcaggag ctgccacagc
      841 ggtatgacgg tcttctcgaa ggtttccaac tctgggtgaa tcttcctgct atgaagaaaa
      901 tgacaacccc tcgatatcga ggcattatcg ctagtcaaat tcccattgtc catcacgagg
      961 atgcaacact aaaggtcatc gcaggaaaag ttgctggtac tcagggacct gtcactgatt
     1021 taatggtcga tatagaatat tatgatgtta cccttgcaaa aggaaaggat tttatccgtc
     1081 cgacaaaaaa agggtacact acgtttctct atgtcatcag cggttctggt tcgatcgttg
     1141 ataaaaaaat cataccccat catggcattc ttctcagcga tggtgatcag gtcaggatat
     1201 cagcagaaga catgctgcgg tttttattca tcaccggtga gcctctgaag gagcctgttg
     1261 cctggggtgg ccctatcgtg atgaatacag aggcggaact gggacaggcg tttaaagagt
     1321 tggacgaagg aacgtttgtc aaaaccagcg ttccagcgtc tctttcgacc ggtttctatc
     1381 gacaatgaac ggcttttcaa gatgatttcc taaggtttct tcccggttac ttttatgctt
     1441 acaaaagagc caccgatttt cttgaattcc tccatggtaa tccctgcttc ttcaatgacc
     1501 gcttgtgtcc gtggtttgtt gatgaccaca tcgacctggt acgaggtttc atctgtcacg
     1561 gtgacatcga tgaagccagc gtccttcagc agctggagat atcggtcttt gaggatcgca
     1621 cctgagatac agccgacata agcttcaacg gagtttttta ttttttcagg gagttctctg
     1681 agcaaaacga tgtctgatac agccaatctc ccattaggtt tcagcacccg aaacgcctcc
     1741 tggaaaacct tttttttgtc tggagatagg ttgatgacgc aattggagat tatcacatcg
     1801 acggaatcgg cttccaaggg gaggtcttca atttccccgc gtctgaactc cacgttggta
     1861 tacccgcttt tttttgcgtt ttccttggct ttctttagca tttcaggtgt catatccacg
     1921 ccgatgacct ttcctgtggg tcctaccttg tttgccgcga ggaaacaatc gaatcctgca
     1981 ccggagccga gatcaaggac gatttctcct tttttcagtg atgcgatggc tgttggattt
     2041 ccgcagccca gtccaaggtt cgctccatcg ggtactgatt gtaattgttc atcggtgtat
     2101 cctattgttt tgctgatctc gaccggtgaa tttgccgtgc cgcagcacac atctatcgat
     2161 ccgcagcagg atgtttcttg ttttgctatt ttcgcgtatc cttctcgtac tttctttttt
     2221 atttttttct cttccatatc actcacacca ttttctttag agtatgtctt tcatgattgg
     2281 accgagtatc cctttgacca tcggttccat atcgtctact ttgattaatg ataaacaaca
     2341 gatttttcca tctttttcca ttgcgactac cgcaagttta tcaccagcgc gaatattggc
     2401 tttttctcga agttcctttg ggagtaccat ctgtcctctt tc
//
LOCUS       DATMXJ010000076         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_48623, whole genome shotgun sequence.
ACCESSION   DATMXJ010000076 DATMXJ010000000
VERSION     DATMXJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48623"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..2147)
                     /locus_tag="VN377_02230"
     CDS             complement(336..2147)
                     /locus_tag="VN377_02230"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HWR27234.1"
                     /translation="MYLQSHSRKKDEKTYTYYSIAESYREDGKNKKKVLSYLGCLTPQ
                     QAQQIRNALKITQSSDTFVVSLDELLFEDHWRYLDVAFLNHLWDKEWGLSSIFPLPED
                     TSKTRRKEISTADVAKILAIYRCLDPGSYLSAVEWLGTTACDSIIGIDGGYFNESRIY
                     RELTAIEQQKEAIEQWLYQKLMNRSEESMRIIFFDLSDSYFEGQECALAKPGRTKAHG
                     FKEKRIILSLLVNSEGYPFSWEILDDYTSDVRTLKGNAIRWMQRFKFPRLIMVFDRGM
                     VSDENLRYLEESDNYLYITALDKDQLAGIDGANLERFESFSKDTTKDEIVSKGLSRYD
                     DSTYYEDLGVDCSGRRHVLVFNLDLLRAQRKTRQKLIEMAFEDLKKEKEELLNAKKSR
                     RLKPTEQRIDEKLEKLKMRSYVGYGLENVNLHGKNNSHVSSFNLEYWRKTNDIQKAQL
                     RDGIWMIVTNISDTMEPKEYRLGPEELISAYRGKNQIEEAFKEVKSFLKFQPTFVYTD
                     EHVRAHYTICILSYLLDMTITNKLRERPIDEVGSARKVHKTLSRCELGKVSVNGTNRS
                     VLRLMPLRADQKSIMELFNCSYLAQSSYLRSIGAEGM"
BASE COUNT          617 a          554 c          473 g          774 t
ORIGIN      
        1 gtgtactttt tagggaaatc gtcttacaga atctcttcac ctatgaaggc tctatccctt
       61 ccattcccta tggttgaacg acctcgtaat aaattcccaa aaagtaaaca ttctccctac
      121 attgatggta ttcatataat aggggatttg tagacttttt agggaaatct tcatcttaac
      181 cttattgagt gattgatatt gatttgctac catgaactag gatcaacttg gacaaaacta
      241 ggatgaaatt gccggtaaaa tctcctatat cccagcttcg tcagattcaa accaaaaacg
      301 ctagctataa aacctacgct tttttaaggt ctgttctaca tcccctccgc gccgattgat
      361 ctcaagtagc tactctgtgc aagatagcta caattaaata gttccattat gctcttttga
      421 tcagctctta gaggcatgag tcttaatacg ctgcggtttg taccatttac acttactttc
      481 cccagttcgc atctactcag tgttttgtga acttttctcg cgctgccaac ctcatcgatc
      541 ggtctttctc tgagcttgtt tgtaatggtc atatccaaca aataggatag gatgcatatc
      601 gtgtagtgag cgcgaacatg ctcgtcggtg tagacaaaag tcggctggaa cttaagaaat
      661 gatttaactt ctttaaacgc ctcttcgatt tgattcttgc cccgataagc agagatgagt
      721 tcttccggac caagccgata ctcttttggt tccatagtat cggagatgtt tgtaacgatc
      781 atccagattc catccctcag ttgagccttt tgaatatcgt ttgtctttct ccagtattcg
      841 aggttaaaac tcgatacatg tgagttgttt ttcccatgaa gatttacatt ttcaaggccg
      901 tatcccacat aacttctcat ctttagcttc tcaagttttt catcgatcct ctgttcagta
      961 ggcttcagcc ttctgctttt ctttgcgttc agaagttctt ctttttcctt cttcagatcc
     1021 tcaaatgcca tttcaatgag cttttgcctt gtctttcgct gcgccctaag aagatccaaa
     1081 ttgaagacca atacatggcg cctgccgctg caatccactc ccagatcttc atagtaagtg
     1141 gaatcgtcat atctggacag acccttcgaa acaatctcat cttttgtggt atcctttgaa
     1201 aaactctcga acctctccag gttggcgcca tcgattccag caagctggtc cttgtccaac
     1261 gcggtgatgt aaagataatt atcgctctcc tctagatacc ggagattttc gtcagagacc
     1321 attccgcgat caaaaaccat gatgagtctc ggaaacttga accgttgcat ccaccggatg
     1381 gcgtttccct ttagcgttct gacatcggat gtgtaatcat cgaggatttc ccaggaaaac
     1441 ggatatcctt cagagttgac gagcagggaa agaataatcc gcttttcctt gaatccatgg
     1501 gccttggttc gacctggttt cgcaagcgca cactcttgcc cctcaaaata tgagtctgag
     1561 agatcgaaaa agatgattcg catgctttct tcggatcgat tcatcagctt ctggtaaagc
     1621 cactgctcaa tcgcttcctt ttgctgctct atcgcggtga gttctcggta gatccgggat
     1681 tcgttgaaat accccccatc tatcccgata atgctgtcac aagcagttgt tccaagccat
     1741 tccactgcag ataaatagct tccagggtct aggcatctgt aaatagcgag aattttagct
     1801 acatcggcag tagaaatttc cttcctccgg gttttgcttg tatcttcggg taacgggaat
     1861 atactcgata gaccccattc tttgtcccat aaatggttca gaaaagccac atcaagataa
     1921 cgccaatggt cttcgaagag cagttcatcg agtgagacaa caaatgtgtc tgatgattgt
     1981 gtaattttta gggcattgcg tatctgctga gcttgctgcg gtgtgagaca tccaagataa
     2041 gatagcactt tctttttatt ctttccatcc tctcgatagg actctgcaat ggaatagtac
     2101 gtgtaagttt tttcgtcctt cttccgtgag tgtgattgta ggtacatggt ctgttctcac
     2161 ttattggttt gtaacagcat gtataaatgc ttttctatta agagtctgtt ctacaaaatt
     2221 tttttaaatg attatttttt aaaagagaat tcgatagctt caaagacatt gggtggctat
     2281 taaaacacaa aaaattagta atctacgcgg gaatctggag aacatgggtt ctcagataga
     2341 atatctccaa cattattgac ggcttggcag acattttgga ccgcttcgtg cacgcctttg
     2401 atgtcctcac gggcaccc
//
LOCUS       DATMXJ010000077         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_43430, whole genome shotgun sequence.
ACCESSION   DATMXJ010000077 DATMXJ010000000
VERSION     DATMXJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43430"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..769
                     /locus_tag="VN377_02235"
     CDS             161..769
                     /locus_tag="VN377_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27235.1"
                     /translation="MNARVKKKILAVEMVMLLIVSGSVGYVIVSRQGSVDGKNENGQH
                     DEIIQDTIKTTDELPPSLQAHIKAGPLTGYGPLAVAFYGNPENDSDIVSYHWEFSPTT
                     MPIIPQAQYKKVHFSVVLFFLFAVVFFPLSFAYMLLYSVFSNHRYKASSQYESTERNP
                     TMIFLYTGSYSATLTITDTQGNTSSDIVWITVLQYVHPDDND"
     gene            complement(837..1514)
                     /locus_tag="VN377_02240"
     CDS             complement(837..1514)
                     /locus_tag="VN377_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013129195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWR27236.1"
                     /translation="MSEVYVGTSGWSYDWNLGNSLGWYIRESQLNAIELNMSFYRFPY
                     PNMVKSWATKGKNLAWVVKVHRSITHFQKLSNTAVEGFQRFKTRFTPMEDLIHYYLFQ
                     LPPSFTDLSTLEQFIEKTGTEKFAVEFRHPALFTNELIAWGKQHDVLLVSIDAPQLPR
                     TIMSKEIVYERIHGRTAWYSHDYSDQELQEIKKRILTGHPKTLYVFFNNNHAMMQNAI
                     RMYHLFR"
     gene            1666..2589
                     /locus_tag="VN377_02245"
     CDS             1666..2589
                     /locus_tag="VN377_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27237.1"
                     /translation="MKTFTLVWKKLVIGIIAVFLSMALIPVTESLPIEKEQSMMTRGC
                     SDGIILNGTMGQNNWYISPVTITFATGNRTFLKIDGGGWSEYTVPIVIDTEGFHEVWW
                     YYIDEHGNQSSIYSTSFKIDMTPPTITITKHRIGLFKYSFTINVSDNTSGVVLLEFYL
                     DDALVGTVTVLPWVYVMEVFGNHAIKVIAYDAAGLNGSDSASTSCSLIYVQQSTISDT
                     PILPPIDRTYFIGQIHNLTIEGNDYHFESTNIRKFGFWRYSIRSWGFSYDHYRGHYSC
                     GWGGAEFHGLLKPDFIWGYFTFLQQGQAESN"
     gene            3055..4521
                     /locus_tag="VN377_02250"
     CDS             3055..4521
                     /locus_tag="VN377_02250"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27238.1"
                     /translation="MGRKAMRRNQHGRNCVMIGIILLFIGASIVPSMSGTTDDRKNDV
                     LQLNESSRADVIITDSLEDVCSVDYMTQETIVVTSHPDIEVDNLDIVEATFTKQGAQV
                     ILSVQVVGLIENRGKTIDLYNEDPLGNLNFVEYDFQVATSEQDYTISYVNLTGQISYG
                     IETTNLTSSDYSVVGDTLSIWFSLTSAEEIYESFSTTAMFVKMNLTGEYPDLENFVYL
                     SDIAPNPPLEIEEVYAPDTGYTGKPIQFNLMIIPCTGTQPFVYYWEFGDGCTSAQKNP
                     THVYTNIGDYTYNVTVTDESGASENYSGVITVVRMQKAILFGRFMWVTSDSDYTTIEA
                     VNLRMIPFNPFEYRHFIDGEQITFSNQYLGIIIGNQVYQILFGIFDVSVGSAPPQTPN
                     IACTTDSTLNRITVATADFDIKWRDIAITLDQPGASYQVFSANGTAIAPVNTTALAGA
                     AEVLAGDYIQLSTYSGNVRTTLRYIPTNSLLGTWTVNV"
BASE COUNT         1347 a          960 c         1065 g         1454 t
ORIGIN      
        1 gtgtcatccg catatgaaga acgattctgc taacgtagtt aaattgtgta aactatttac
       61 tgatagttat atttatttat taaaacgaag atagataact catattattc ctacaaaatg
      121 gttaacgaat ccaaatttaa aaaagggagg atacacgtct atgaacgccc gagtaaagaa
      181 aaaaatactc gctgttgaga tggtaatgct gctcatcgtc tctggatcgg tcgggtacgt
      241 tatcgtatca agacagggat cggtagatgg taaaaacgag aatggacaac atgatgaaat
      301 tattcaagat acgataaaaa ctaccgatga actgccaccg tcccttcagg cgcatataaa
      361 agcaggcccg ctcaccggat atgggcccct ggctgttgcc ttttatggaa atcctgagaa
      421 tgattccgac atcgtttctt atcactggga gttcagtccg acaacgatgc ctatcattcc
      481 tcaagcacaa tacaaaaagg tgcatttttc cgttgtcctc tttttccttt ttgctgttgt
      541 ctttttccct ctaagttttg cgtatatgct tctctactcg gttttctcca accatcgata
      601 caaagccagc agccagtatg aatccacaga acgtaatcca acgatgatat ttctgtacac
      661 aggaagttat tcagcaactc ttacaataac cgatacacaa ggtaatactt cttcagatat
      721 cgtgtggata acggtattgc agtatgtgca tccagatgac aatgattaat gacatgcgtg
      781 acaacggata tttggtacat tctctgctct ttctcagata tcgtctcaaa agttaatcat
      841 ctgaaaagat gatacattcg tatcgcattt tgcatcattg catggttgtt gttgaaaaac
      901 acatacaggg tcttggggtg tcccgtaaga atacgttttt ttatctcttg gagttcttga
      961 tctgagtaat catgggagta ccaagcggtc cgtccatgga tccgttcgta gacgatttct
     1021 ttgctcatga tggtccgggg aagctggggt gcatcgatgc tgaccagcag aacatcgtgt
     1081 tgttttcccc atgcgatgag ctcgttggta aacaaggcag gatgtcggaa ctcgacagcg
     1141 aatttctcgg tccctgtttt ctctatgaac tgctccagtg tggaaagatc ggtgaaggat
     1201 ggcgggagct ggaagaggta gtaatggatc aggtcttcca tgggggtgaa gcgtgttttg
     1261 aagcgttgga agccttcaac agctgtgttg ctgagttttt ggaaatgggt gatggaccgg
     1321 tggactttga cgacccatgc gagatttttc cctttcgtcg cccaggattt gaccatgttt
     1381 gggtagggga accggtagaa gctcatgttc agttcgatgg cgtttaactg cgattctctg
     1441 atgtaccagc cgagagagtt tcccaggttc cagtcatagg accatcccga tgtgccgaca
     1501 tacacctcac tcatctatgt cgagaacgat tgttgtcttc ttattctttt ttctgttggt
     1561 tcatcaccgt tttcgcttgt ttttctcagg ttctgaagaa aatttactat caataatgta
     1621 tttataggcg aagattgtta taaatttgta acggggtgga aagaaatgaa aacatttact
     1681 cttgtttgga aaaagctggt tattggaatt attgctgtgt ttctgagtat ggcgctcata
     1741 ccagtaacgg agagtctccc aattgaaaaa gaacaatcaa tgatgacgag gggttgctca
     1801 gatggcatca ttctcaatgg tacgatgggg caaaataact ggtacataag tcctgtcact
     1861 ataacttttg ccactggcaa tagaactttt ttaaaaatcg acgggggagg ttggtctgaa
     1921 tatacagtac caattgtcat tgataccgag ggatttcatg aggtgtggtg gtactatatt
     1981 gacgagcatg ggaatcaatc ttctatctac tcaacaagct tcaagattga tatgacgcct
     2041 ccaacaatca caattacaaa gcatagaata gggcttttca agtattcatt tactataaat
     2101 gtctctgaca atacaagtgg tgtcgttttg cttgaatttt atcttgatga tgcgctcgtt
     2161 ggtactgtta ctgttttacc ttgggtatat gtgatggaag tatttggaaa tcatgcgata
     2221 aaggttatcg cttatgatgc tgcggggttg aatgggagtg actcggcgag cacctcctgc
     2281 agcctaatat atgtacaaca atctactatc tctgatacgc ctattttacc tccgatcgat
     2341 aggacctatt ttatcggaca aatccataac ttaacaattg aaggcaatga ttaccatttc
     2401 gaatccacta acatcaggaa atttgggttt tggagatata gtatccgctc ctgggggttc
     2461 tcctacgacc actatagggg tcattattcg tgtggctggg gtggcgcaga attccatgga
     2521 ctattaaaac ccgattttat ttggggatat tttacttttt tacaacaagg gcaggcagaa
     2581 agtaactgat caacaatgga agtcgtgatg ccaaaggatg tctcaatcga tggtttcctc
     2641 ttctggttct tcgaagttct cccgcatacg tttccgatac ttcgacattt cttttggaac
     2701 taaaaatccg gattttttcc ttctttaatt tgtttataaa aaaaaggcac tactctgact
     2761 gatttttctc ttattttccc tctttataaa ccaggaatcc gtaggtgatg ttgccgcacc
     2821 gaggacagca caggaccccg tccctatatg ttgtttcggt agcatctttt ctgctcagtt
     2881 cctcaaaata gttctcacac catctgcata catacgtcat gcttctctcc tcgcaagttc
     2941 aaatcatatc gtttgtctta aattttttgg atggaatcag cgattgaaac tcggtattat
     3001 aaagaaaagt tctacagata ggtttttaag gggaaacaac gttataaacg tataatgggg
     3061 cggaaagcaa tgagacgaaa tcagcatgga agaaattgtg tgatgattgg gatcattctg
     3121 ctattcatag gggcgagcat cgtaccgagc atgagtggaa caactgatga tagaaagaat
     3181 gatgtccttc agttgaacga gtcaagtcgt gctgacgtaa taattactga ttctcttgag
     3241 gacgtctgtt cagtggacta tatgactcaa gaaactattg tggtcacctc tcatcctgat
     3301 atcgaggttg acaatcttga tatcgttgag gcaacgttta ccaaacaagg cgcgcaggtg
     3361 attctgagtg ttcaagtggt cggattgatt gagaacagag ggaaaaccat tgatttatac
     3421 aatgaggatc ctttgggtaa ccttaatttt gtcgaatatg attttcaagt ggccacgtca
     3481 gaacaagatt atactattag ttatgttaac ctgaccggtc aaatcagcta tggcattgag
     3541 acaacgaatc tgacttcttc tgattattcg gttgttggtg atacgctttc catctggttt
     3601 tcattaacaa gtgcggaaga aatctatgaa agtttcagta cgaccgccat gtttgtgaaa
     3661 atgaatttga ccggtgaata cccggacctc gaaaacttcg tctatctctc agatatcgct
     3721 ccgaatccac ccctggagat tgaggaagtg tatgccccgg acaccggtta tacgggaaaa
     3781 cctattcaat ttaacctcat gataattcca tgcacaggaa cccaaccatt cgtgtactat
     3841 tgggaattcg gggatggatg cacctcagcg cagaagaatc caacacatgt ttacacaaac
     3901 atcggagact atacatacaa tgttacagtg accgatgaat cgggggcatc agaaaactat
     3961 tctggtgtta taactgtcgt tagaatgcag aaagcgatcc tctttggacg attcatgtgg
     4021 gtaacatcag atagcgatta cacaaccatc gaagctgtga atctgcggat gattcctttc
     4081 aatccctttg aatatcgtca ttttatcgat ggagagcaga taacattttc gaatcaatat
     4141 cttggaataa ttatcggaaa tcaggtgtat cagatcttgt tcgggatctt tgatgtctct
     4201 gttggttctg ctcctccaca gacgccgaat atcgcatgta cgaccgattc aacattgaat
     4261 aggataacag tcgctaccgc agatttcgac ataaaatgga gggatattgc gattacgttg
     4321 gatcaaccag gtgcatccta tcaggttttc tctgcgaacg gtaccgccat tgctccagtg
     4381 aatacaacag ctttagctgg tgctgcagaa gttcttgctg gagactatat ccaactctcg
     4441 acgtacagcg gtaatgtaag aacaacgtta cgatatatcc cgaccaattc attgctcggc
     4501 acctggactg taaacgtcta aatcaaagga tctcttattg tgaatatgtc actttttttt
     4561 atagttgcga ctgagaatac tgcgtggtct gaaacaaagt gaaagacccg cagatgaaaa
     4621 gtcgcatctc tatttttctc cagctaaagt atatctcttt tttgtcgcac gcggcaggca
     4681 ttcgcctgcc ctctaaaggt gcgacaggag aaaatatttg tatggaaaat gtaattagct
     4741 gtagccctaa aaagattgcg ggagatactc gcaataactg ggtgaccatg atgttcgtca
     4801 cccacaacag atacaactgt tttaga
//
LOCUS       DATMXJ010000078         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_6671, whole genome shotgun sequence.
ACCESSION   DATMXJ010000078 DATMXJ010000000
VERSION     DATMXJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6671"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VN377_02255"
     CDS             <1..389
                     /locus_tag="VN377_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27239.1"
                     /translation="LGLNAKADAIEVEKIGVNIKRYPKDDGSWSFPQRIDMNGPFFRI
                     TSLKTSEIARKIRDADIPAKQSFFAGSYICNALFYGVLGYVKEQNLNTSVGFIHVPLL
                     DSQDSAGMPLESMVEAVKIAVQECLV"
     gene            427..693
                     /locus_tag="VN377_02260"
     CDS             427..693
                     /locus_tag="VN377_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27240.1"
                     /translation="MREITKAQVTIGLLAFFATIWDVLINGSYPNWFYTIGIAVLQLV
                     LLLILSYVIIAMLEWVKVFDLKTNSILTLLVLVTLKLLTILKIS"
     gene            complement(711..1637)
                     /locus_tag="VN377_02265"
     CDS             complement(711..1637)
                     /locus_tag="VN377_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWR27241.1"
                     /translation="MESYQEELTQIKTILKNNPKGMTVSDIAHEIHTNRNSVAKYLDI
                     LLISGHVEMVTFGPAKVFFPSSRMPLTALLNFTHDYIALLNRELKFLQVSENLLKFIE
                     IQRSEIIGRPIQTFSVSFFGIPELAQNAKKALEGTEHSIEKQYIQKNQLFYLRIKHIP
                     TTFDDGEPGVTLVIEDITDQKQTEAKMKRTIQEWEITFNSISDMVFIQNNDFSIIRAN
                     RTFAEFLHMTPEECVGKKCHQLLHSKNESPSFCPGPQVQKTKKTTTVEFYEPHLDRYL
                     EITASPILTDSGEITGSVHVMKDITERKRKEG"
     gene            2099..2506
                     /locus_tag="VN377_02270"
     CDS             2099..2506
                     /locus_tag="VN377_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR27242.1"
                     /translation="MNEVRYYQKNLMIVDDDPDIVMSLQELFEREGFQVTTADNGRNC
                     LLELEKGFQGIIILDLMMPIMDGIETIKKMTIDGFTDENTIIVLTAKRIQGEEFNEIY
                     PYIADYITKPFDISALLHAVKKIAQKPPVKRRY"
BASE COUNT          686 a          500 c          568 g          754 t
ORIGIN      
        1 gtcttggtct gaacgcgaaa gccgatgcca tcgaggtaga aaaaatcggg gtgaatatca
       61 aacgatatcc aaaagacgat gggagttggt cgttccctca gcggattgat atgaacggtc
      121 catttttccg tatcacctct ctaaaaacaa gtgagattgc tcggaagata cgggatgcag
      181 atattcctgc gaaacaaagt ttctttgcag gatcgtatat ctgcaacgcg ttgttctatg
      241 gagtattagg ttacgtaaaa gagcagaacc ttaacacctc agtaggtttt atccatgtgc
      301 cgttattaga ttcgcaggac tctgcgggga tgccactgga gagtatggtg gaggcggtaa
      361 aaattgccgt tcaggaatgt cttgtatgaa taatctttat atagaaaaca tgtactgaca
      421 acatcgatga gagagataac aaaagcacag gtcaccattg gtttgttagc cttttttgca
      481 acgatatggg atgttcttat caatggatcg tacccgaatt ggttctacac catcggcatt
      541 gctgttctgc aactggtatt gctgcttatc ttatcctatg tgatcatcgc gatgctcgaa
      601 tgggtcaagg tctttgattt gaaaaccaat tcgattctga cgttactggt gttggtgacg
      661 ttgaaactgc tgaccatcct taaaatttca taaaaaaacg gatggtttac ttaaccctct
      721 tttcgctttc gttcggtgat gtccttcatg acatggacag aaccggtgat ttcaccgctg
      781 tctgtcaaaa tcggtgaggc ggtgatctct aggtatctgt cgagatgcgg ctcgtagaat
      841 tcgacggtcg tggttttttt tgtcttttga acttgtggac cggggcagaa ggagggtgat
      901 tcgttcttgc tatgcaggag ctgatggcat ttttttccga cgcattcttc tggcgtcata
      961 tggaggaatt ctgcaaacgt gcggtttgcc ctaatgattg aaaaatcatt gttctggata
     1021 aagaccatgt ctgaaatgga attgaatgtg atttcccatt cttggatggt gcgtttcatt
     1081 tttgcttcgg tttgtttttg atcggtgatg tcctcgatga cgagggtgac gcctggctca
     1141 ccgtcatcaa aggttgtcgg gatatgtttg attctaaggt agaagagttg gtttttttgg
     1201 atatattgtt tttcaatgct gtgttctgtt ccttcgagtg ctttttttgc gttttgtgcg
     1261 agttcaggga ttccaaaaaa tgaaacagaa aatgtctgaa ttggtcttcc gatgatttct
     1321 gagcgttgga tttctatgaa ttttaagagg ttttcgctta cttgcaggaa ttttaattcg
     1381 cggttcagta gggcgatgta gtcatgggtg aaattgagta gggcggtgag tggcattctt
     1441 gatgaaggaa aaaatacttt tgctgggcca aaggtaacca tctcgacgtg ccctgagatg
     1501 aggaggatgt cgaggtattt tgcgaccgag tttctgttcg tgtggatttc atgggcgata
     1561 tcgctgacgg tcattccttt tgggttattt tttagtattg tttttatttg tgttaattcc
     1621 tcttgataac tctccacgtc cgattatcta tatctgtata tttatgcatt tcgttattgc
     1681 caatcagtaa tgccatgcaa agaaagaaaa gtatttatat aacatagatg atgctttcat
     1741 tgccatgcag aaacgcatgg caaagtggtg aagggataaa aaaatatgac ttctcctcta
     1801 ataatactaa accgcgatca tcccatcaaa acatccgcct gggggagcat accaccttca
     1861 aacaaaaaac caattcacag cgatcctcct catctcttcc cacatcccat ccttccccaa
     1921 acacccgata agaaaaaatc cttcctatat ccatgaggta cctcgggcat tcaaagcatc
     1981 ccatctctcc ttttcccaga ggtacctcct cccatccaat cgacccagat ggatttttgc
     2041 tggttttgac atttgaacaa agtaatttta tatagtaaaa taatgaagat atacacctat
     2101 gaacgaggta cgatattatc aaaagaacct tatgattgtg gacgacgacc ctgatatcgt
     2161 catgagcctt caagagcttt ttgaacgaga aggatttcag gtcaccacgg cagacaacgg
     2221 aagaaattgc ctcctcgaac tggaaaaagg ctttcagggg atcatcatcc tagatctgat
     2281 gatgccgatc atggacggga tagaaaccat aaagaaaatg accatcgatg gattcaccga
     2341 cgaaaacact ataatcgtac tcacagcaaa acgcatacaa ggggaagagt tcaatgaaat
     2401 ctacccctat atcgctgatt acatcaccaa gccatttgat atcagcgccc tgctgcatgc
     2461 cgtgaaaaaa atcgcgcaaa aaccaccggt caaacgacga tactaaca
//
LOCUS       DATMXJ010000079         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53967, whole genome shotgun sequence.
ACCESSION   DATMXJ010000079 DATMXJ010000000
VERSION     DATMXJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53967"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VN377_02275"
     CDS             <1..709
                     /locus_tag="VN377_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27243.1"
                     /translation="AEMTQLKTMLKWDIILQSRYKIIHLSILSVLIYFVTLIAIPSMN
                     TEEFKTLYLFFDPTLIGIMFVGALVLFEKTENTLQALNVTPMETRTYFFSKIISLTLL
                     SVISGFLFLFLSHGIHFEYFFMVTGIILTSVFLILLGFILVARCNSINEYLLMMMLAF
                     LLLFLPPLLDITGIYNNIVFYLWPSQASFLLFEGVFGSLSLNETIYALAYLSIWIVGC
                     YYLAKKAFYKYIVLGGS"
     gene            712..1422
                     /locus_tag="VN377_02280"
     CDS             712..1422
                     /locus_tag="VN377_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27244.1"
                     /translation="MVQIRKLLVNDIRNIIRDRLLLYSAFLIPLIIIIFTRLVIPWLS
                     ENVVPLEQFYPLLFMLIVITIPLLFGFVIGFLIMDERDENLLTVLRVMPISRNTYLIY
                     RMMFISLLSLVYILLFPTLTGLIDINFIDYLPIGLLFAFLTPTIGLVANIVATNKVQA
                     FAVFKTLGGVFYIPLFAFFIDNDLKYILGIIPNFWTFMALDKVLTTGTQDYVFLGIGF
                     CLHLVLLGLLFYLFNKKN"
     gene            complement(1586..2392)
                     /locus_tag="VN377_02285"
     CDS             complement(1586..2392)
                     /locus_tag="VN377_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27245.1"
                     /translation="MKKHLIVIGLISVVLSGCFSGCVGPEVTDYFNGEYPVDEQTIVS
                     VSNINGQVEITGWAGDNVTVSAVKTSTLGEEELHNVNISVNQIGNHLEIETKYTGQKF
                     IQAGVDYSIKVPFNTTIGTIMTSNGGIQISNTKGDIIASSSNGAIIMENVDGVVSATT
                     SNGHIEILNTTGIRDIRTSNAAITAEVQSIPDDVSIETSNGAVTVYVNPLLNATLEMA
                     TSNAKVKLQEITLNINLLEDTHVIGTLGMDGQKIDIHTSNANIYLNKLQT"
     gene            complement(2436..3365)
                     /locus_tag="VN377_02290"
     CDS             complement(2436..3365)
                     /locus_tag="VN377_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27246.1"
                     /translation="MHRSFGLSILGGLLLTYPLYLGHAVFLLNVAFLWKKTSPRQLYF
                     FGMLFGLYEALITKVLWFGYPASTGPMVGYFFGVAWGEFLTLVLFYHPIMSFVVPIFV
                     YEVLSGDVLPGHERFLQRNWKTLLLVMILIVMGASFQSNGAGYDLVQSVGSMGGSILI
                     VVLLHFLSRGKNLQSLVLGKKGLTIVLMYLFVLYGVTTVVIFPERLPKIVIAYVLIVL
                     WYLVAIGLLWVDRFTPAYISVKRTMFSQKELGIFAIVVVGATVVCSVFQQVTYGVLLT
                     MFVSLMGAGVILFIWSVFSLMRQRISQKLSEDL"
     gene            complement(3512..3982)
                     /locus_tag="VN377_02295"
     CDS             complement(3512..3982)
                     /locus_tag="VN377_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWR27247.1"
                     /translation="MDENECCPKFDPTPWDGKVFEWNNKKFIKENVSTFFHMPLNFGG
                     AMKRLDEKVRDAHGAILDGICLSDHTSKWNMDLYLAVDKEIPHAENTTISGKFLSKVY
                     EGPFQDTGKWCKDFEQFASSKGMKVSKWFMWYTTCPKCAKKYGKNYVVIVAQVT"
     gene            complement(3982..4482)
                     /locus_tag="VN377_02300"
     CDS             complement(3982..4482)
                     /locus_tag="VN377_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWR27248.1"
                     /translation="MAYKKKSWQEKLADKKNLPKVLKLQKSFPCYNAVHKMGAETGDD
                     VVLVNPSEVVDRMKQVPKGKLITLAEICKAIAKKHHAKGCCSLTTGIFIMTAANAAEE
                     AANQGKAFPVPYWRTLKIDGFLNEKYPGGALSQKKLLEAEGFTILKKGKKFVVKDFER
                     YRAEVK"
     gene            complement(4487..4891)
                     /locus_tag="VN377_02305"
     CDS             complement(4487..4891)
                     /locus_tag="VN377_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HWR27249.1"
                     /translation="MNKHMVSFCGYRCDLCPAYVKNVDTLAKRSAIRKGWNTFFGFDV
                     PEERIICDGCDSTGNHLDTECLVRPCALSKHLQNCSFCDSFESCETLRTRADILDTTK
                     TKYSGQISKEEYALFFRPYEGRDELKKQRKAR"
BASE COUNT         1362 a         1231 c          925 g         1514 t
ORIGIN      
        1 agctgaaatg acgcagctta aaacaatgtt aaaatgggac atcattcttc aatctcgtta
       61 taaaattata cacctttcca tcctgagcgt cctgatctac ttcgttacac ttatcgcgat
      121 cccatcgatg aacaccgagg agttcaagac cttgtattta ttcttcgacc cgacgctcat
      181 cggcatcatg ttcgtcggcg cattggtcct ttttgaaaaa acagaaaaca cgctgcaagc
      241 tctcaacgtc actccaatgg aaacaaggac atatttcttt tcaaagatca tctcccttac
      301 gttactgtct gtcatctccg gttttctctt cctttttctt tcccatggga ttcattttga
      361 gtatttcttc atggtgactg ggattatctt gacttctgtc tttcttatct tacttggatt
      421 catccttgtg gcacgatgta acagcatcaa tgaatatctg ttgatgatga tgcttgcgtt
      481 cctcctgctc ttcctcccac cattactgga cataaccgga atctataaca atatcgtctt
      541 ttatctttgg ccatcacaag catcgttcct tctctttgaa ggtgtttttg gctcgctttc
      601 gcttaatgaa accatctacg cacttgccta tcttagtatc tggatcgtcg gctgctatta
      661 tctcgcaaaa aaagcattct acaaatatat cgtgctcggg ggaagctaac catggttcag
      721 ataagaaaat tattagtcaa cgatatcaga aatatcatac gagaccgttt actgctgtat
      781 tccgcgtttt tgataccact gatcattatt attttcacaa gacttgttat tccctggtta
      841 tcagaaaatg ttgttccatt agagcagttc tatccgctgt tgtttatgct tatcgttatt
      901 accatcccgt tgttgtttgg ttttgtcatt ggatttctca tcatggatga acgtgatgaa
      961 aacctgctga cagtcctgcg tgtgatgccg atttcaagaa acacctacct catctatcgg
     1021 atgatgttca tcagcctact ctctctggtc tatatcctgc tctttcccac cctcaccggt
     1081 cttatcgata tcaattttat tgattatctc cccattgggc tgctgtttgc attccttaca
     1141 ccgaccattg ggctggtggc aaatatcgtt gctacgaata aagtccaagc cttcgccgtg
     1201 ttcaaaacac ttggaggcgt cttctacatc cctctgttcg ctttcttcat agacaatgat
     1261 ctcaaatata tccttggtat catcccgaat ttctggacat ttatggctct ggataaagtg
     1321 cttacaaccg gaacccagga ttatgtcttc ctcggaattg gattttgcct gcatctcgtc
     1381 ttgcttggat tactcttcta cctcttcaat aaaaaaaatt gaagatggta ttgctttcta
     1441 aatgaccctt gcagaggatt aaccctgcaa cgatactcat cattgctacg tagtccggat
     1501 aactacctca ccgtcctggg aaaatgaaga ggaaataaca atcgacatca gaagggatgt
     1561 tatctcttac tgtcgaatgg gactgttagg tttgtaattt gtttaagtag atatttgcat
     1621 ttgatgtatg gatgtctatc ttctggccat ccatacccaa tgtcccgatg acatgggtgt
     1681 cttctaacag attgatgttc aaagttatct cttgcagttt tacttttgca ttggatgtgg
     1741 ccatctccaa tgtcgcattc agcagggggt ttacataaac ggttaccgcg ccattgctcg
     1801 tctcgatgct gacatcatcc gggatgcttt gcacctctgc ggtaatagcg gcatttgatg
     1861 tacgaatatc gcgaatcccg gtggtattca ggatttcaat atgtccgttg ctcgtcgtcg
     1921 ctgaaacgac accatcgaca ttctccataa tgatcgcacc gttggaactt gatgcaatga
     1981 tatctccttt tgtattggaa atctggatac ccccattgga ggtcatgata gtccctatag
     2041 tggtattgaa cggtactttg atagagtaat caaccccagc ttgtatgaat ttctgaccag
     2101 tatattttgt ttcgatttcg aggtgatttc ctatttgatt gacgctgatg ttgacattgt
     2161 gaagctcctc ttctcccagg gttgatgttt tcacggcaga gaccgtgaca ttgtcaccag
     2221 cccatccggt tatctcaacc tgaccattga tgtttgaaac actcacaatg gtttgttcat
     2281 cgacaggata ctccccgttg aaataatcgg tgacctctgg tccaacacaa ccactgaaac
     2341 atccactgag aactacactt attaaaccga tgacaatcag gtgttttttc atttcatatg
     2401 cctccgtaaa gtattcacgg tattcctcat tttactcata aatcttctga taatttctgg
     2461 gaaattcttt gcctcatcaa tgaaaacaca gaccagataa aaagaatcac tcctgccccc
     2521 ataagtgaca caaacatcgt aagaagaacc ccgtacgtta cctgctggaa aacgctgcag
     2581 accacggtcg cacccacaac tacaatagca aaaatcccca gctctttctg agagaacatc
     2641 gttcttttca cagaaatata cgcaggagtg aaccgatcga cccagagcag accgatagcg
     2701 accagatacc acagaacgat tagcacataa gcaataacta ttttcgggag acgctcaggg
     2761 aaaataacca cggttgtcac accatacaaa acaaaaagat acattaaaac aatggtcaat
     2821 ccttttttcc caaggacaag cgactgcaga ttctttcccc ttgaaaggaa atgtaataga
     2881 acgactatca agatagatcc acccatggaa cctacagatt gaaccaaatc atatcccgca
     2941 ccgttgctct gaaaacttgc ccccatcacg atcaatatca tgaccaaaag aagtgttttc
     3001 cagtttcttt gtaaaaaccg ttcatgacct ggaagaacat ccccgctcag tacctcatac
     3061 acgaaaattg gtactacaaa agacatgatc ggatggtaga acagcaccaa ggtgagaaac
     3121 tcaccccacg caacaccaaa aaaataacct accatgggac ccgttgaagc tggatagcca
     3181 aaccagagta cctttgtgat aagtgcttca tagagtccga acagcatccc gaagaagtac
     3241 agctgacgag gcgatgtctt tttccacaga aacgctacat tcagcaagaa taccgcatgt
     3301 ccgagataca aggggtatgt gaggaggagc cccccaagga tcgacaaacc aaagcttcga
     3361 tgcacccgag aatatctctg caaacagcat tgagagtgca ccaagaagaa gaatcgacca
     3421 caacttcctc atattagaaa tatcattatt tcgttattta atcataccga caaaaagttt
     3481 tgggaatcat aaaagatatc agcagaaaag actacgtgac ctgagcgaca ataactacgt
     3541 aatttttccc atattttttc gcacatttcg gacatgtggt gtaccacatg aaccattttg
     3601 aaaccttcat acccttgctt gaggcgaact gttcaaaatc tttgcaccat ttcccggtat
     3661 cctgaaatgg gccttcgtac actttgctga ggaatttacc gcttatcgtc gtattctctg
     3721 catgaggaat ctccttatcg accgcaagat acagatccat attccacttc gacgtatggt
     3781 ctgataagca tatgccgtcc aggattgctc catgagcatc ccgtaccttc tcatcaagtc
     3841 tcttcatcgc accaccaaaa ttcagcggca tgtggaagaa tgtcgagaca ttctccttta
     3901 tgaatttctt gttgttccat tcaaacacct ttccatccca tggtgtggga tcaaatttcg
     3961 ggcaacattc attttcgtcc attatttcac ctcagctcga tatctttcga aatccttgac
     4021 aacaaatttc ttgcctttct tcaatatggt aaatccttct gcttcaagta gttttttctg
     4081 agaaagcgcc ccaccagggt atttttcgtt cagaaatccg tctattttca aagtccgcca
     4141 ataggggacg ggaaacgctt ttccctggtt agccgcctct tctgctgcat ttgctgcagt
     4201 catgataaaa atccctgtcg tcaaagaaca acatcctttg gcatgatgct tctttgcgat
     4261 cgctttacag atctctgcaa gagtgatgag ttttcctttc ggaacctgtt tcatacgatc
     4321 aacgacttca ctgggattta ccaacacaac gtcatcgcca gtctctgcac ccatcttatg
     4381 gaccgcgtta taacacggga agcttttttg taacttcaga accttcggta aattcttctt
     4441 atcagcaagt ttctcctgcc aggatttctt tttatatgcc atgtgctcat ctcgcttttc
     4501 gttgtttttt cagttcgtct cttccttcat acggacggaa gaacaatgca tactcctcct
     4561 ttgaaatctg accagagtat ttcgtttttg tcgtatcaag gatgtctgca cgagttcgta
     4621 aggtttcaca ggattcaaaa gaatcacaga acgaacagtt ctgtaggtgt ttgctgagtg
     4681 cacaaggtcg aacaagacac tctgtatcca aatggtttcc cgtactatcg caaccatcac
     4741 agatgattcg ttcttcaggg acatcaaaac caaaaaaagt gttccatcct ttccgaatcg
     4801 ctgatctctt tgcaagtgtg tcaacattct tgacatacgc agggcagagg tcacatcgat
     4861 atccacagaa cgacaccatg tgtttattca tcgttccacc ggtacaggtg cttcttcttt
     4921 ctcaaccgat ctgtgtcgca ctcaaaacaa agaccgttat gacaacaaat cacacctccg
     4981 caggtgggac agcgccattt ctcctcttca tccttgagga attgttccat cc
//
LOCUS       DATMXJ010000080         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_17437, whole genome shotgun sequence.
ACCESSION   DATMXJ010000080 DATMXJ010000000
VERSION     DATMXJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17437"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>2256
                     /locus_tag="VN377_02310"
     CDS             173..>2256
                     /locus_tag="VN377_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWR27250.1"
                     /translation="MVDVKKSQRKQVNTNRKKLYILSIIVILISNVFPLMSIVEASIQ
                     DPHLIIDSIPTVTEQVHSNNYIETTTTVTPWWNTNWHYRRIYNITGIGNISLPMNFTS
                     VLRSFQVVNKTFDNSTITIVRYYKNGTIVVVDKTWFNESTVFHNRTNAVGTLSWSVSG
                     TSPYGVYFDVKENRGTRNPMNETRNLTQSGPVHATLISTQGWWPEFINDIPTYYVNNT
                     ILNVEVYTTALAKNLTAHFLCNGIIVSNTSLSTLDNLTWKTTKKLSKIGDWTIHIIGY
                     DDAGYQTAPLTAGFYIGKPDLIASNLTVPICYVGYNVTVTAHIRAVNTTVENVTVALR
                     VNNINVSIKENLKIQKNENRTLQLYWTPSSKGSHNVSFNIFYHPDSNPSNNIIWKNVN
                     VEGVPDLAVLNITVAPTPVDEGNPVMVTAYIRNTGDGNATNYEVVLYCEQNQNNHTML
                     YLQEKNSTNFSLKKNTSTNVTLTWFQTRYGKANFNGEWAVGIKILNTTQTPDKHEANN
                     NKSLYHVLKVIAAERNPPILSNLEYLSSIELGNQLLIRVKATDASGIDMVVISIKTPN
                     KTTVNTTMATEENDRYEYIFTAVQLGRHNFTIKATDLSPNKNQSIITGYFIVTEDQTP
                     PTITYFGVNPLVQLPNHPVETRCITSDYSGIRSVEVAIRFPDNQVETHEMSTPPTDTK
                     YVYTKTYETKGK"
BASE COUNT          800 a          555 c          359 g          542 t
ORIGIN      
        1 atcatatatc gattttcatc tgcttcgtcc caaccaataa tctctctgtg aaacgtagaa
       61 aaaacagaac caaaataaat ataatttctt taattatata taataaatat tcaatagaaa
      121 actacaaata tatcgtcata aatagcaata ctatagaaga tacgagggag ccatggttga
      181 cgtaaaaaaa tcacagcgga agcaagttaa cacaaataga aaaaaattat atattttaag
      241 catcatcgtc atcctcatct ccaatgtatt tcctttgatg agcatcgttg aagcatccat
      301 acaagaccct catctgatca ttgactcaat accaacagtc acagaacagg tacactcaaa
      361 caactacatt gaaacaacaa cgactgtgac accatggtgg aacaccaatt ggcattaccg
      421 gagaatctac aatatcacag gaataggcaa catttctctt ccaatgaatt ttacctcagt
      481 tcttcgatca tttcaggtcg tgaataaaac atttgataac tccaccatca ccattgtacg
      541 atattataaa aacggaacaa tcgtcgtcgt cgacaaaacc tggttcaacg aaagtaccgt
      601 ctttcacaat cgaacgaacg cagttggtac cctctcctgg agcgtatcgg ggacctcccc
      661 ctacggcgtc tattttgacg tcaaagagaa tagaggaacc cgcaatccaa tgaacgaaac
      721 ccgtaacctc acacaatcag gtcccgtaca cgctactctg atatccaccc aaggctggtg
      781 gcctgagttt attaatgata ttcctacgta ctacgtaaac aatacaatac tgaatgtcga
      841 agtatacaca acagcgctag ccaaaaacct caccgctcat ttcctttgca atggaattat
      901 tgtttccaat acatcgcttt ccaccttaga taatttaacc tggaaaacca caaaaaagtt
      961 atctaaaatc ggtgactgga cgatacacat catcggatat gatgatgcag gatatcaaac
     1021 cgctccgttg accgctggat tctatatcgg caaaccagat ctcatcgcct caaacctgac
     1081 cgtaccaatt tgctatgtag gctataatgt cactgtaacc gcacatatcc gtgcggtgaa
     1141 caccacagtt gagaatgtta cagtcgcact tcgcgtcaat aacataaacg tatctattaa
     1201 agaaaatcta aagattcaaa aaaatgaaaa cagaaccctc caactttatt ggactccctc
     1261 cagcaaaggc agccataatg tctcattcaa cattttttat catccagatt caaacccaag
     1321 taataacata atatggaaaa atgttaacgt tgaaggcgta ccagatctcg ctgtgctaaa
     1381 tatcaccgta gcccccaccc cagtagacga aggcaatcct gtaatggtca ctgcatacat
     1441 ccgcaacact ggagatggaa acgccaccaa ctatgaagtc gttctctatt gcgaacaaaa
     1501 tcaaaacaat cacactatgt tatatttaca ggagaagaat tcaactaact tcagtttaaa
     1561 gaagaatacc tcaacaaatg tgacacttac ctggttccag acacgctatg gaaaagcaaa
     1621 ttttaatggg gagtgggctg tcgggatcaa aatccttaac actacgcaga cacctgacaa
     1681 acacgaagca aataacaaca aatctctcta ccatgtccta aaggttatcg cagcagagcg
     1741 aaacccaccc attctttcca accttgagta tctctcatct attgaattag gaaaccagct
     1801 tcttatcaga gtcaaagcaa ccgatgcatc agggattgac atggtcgtta tctcaattaa
     1861 gaccccaaac aaaacaactg tgaacaccac catggcgaca gaagaaaacg atcgatatga
     1921 atatattttt accgccgtac aactcggcag acataacttt accatcaaag caacagatct
     1981 ctcaccaaat aaaaatcagt cgattatcac aggatatttt atcgtcacag aagatcagac
     2041 gccaccaacc atcacctatt ttggtgtcaa tcccctcgtc cagttaccga accaccctgt
     2101 ggaaacccgc tgcatcacat cggattactc agggatacga tccgtcgaag tggcaatacg
     2161 attcccagac aaccaagttg aaacacatga aatgagcact ccaccaactg atacgaaata
     2221 cgtctacacc aaaacatacg aaaccaaagg aaaata
//
LOCUS       DATMXJ010000081         8263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_53976, whole genome shotgun sequence.
ACCESSION   DATMXJ010000081 DATMXJ010000000
VERSION     DATMXJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53976"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VN377_02315"
     CDS             <1..629
                     /locus_tag="VN377_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWR27251.1"
                     /translation="WAGDGGTYDIGIQALSGALERRTNFIYICYNNQIYSNTGIQRSG
                     ATPYAAWTTTTVSGKNEFRKEMGEIILAHHIPYAAQTCISYPEDVINKITKAKNIQGP
                     KYIEILAPCPPGWRFSMDKTVEMGRLAVDSGAWSLWECENDQMTFNGKSKLILEKKVQ
                     RKPVEEWIKYQGRFSHLFKPQRDEERLKAIENYIDNLWDRYRKCYATK"
     gene            complement(661..1533)
                     /locus_tag="VN377_02320"
     CDS             complement(661..1533)
                     /locus_tag="VN377_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27252.1"
                     /translation="MTGIAFAPGHISAFFEPVYSEQNIDRSGSCGAGINITLGAISQV
                     KIQPASSRTVTVRISGKLSNAPVTKSALYYLIGTTPLSVTVNTVLDLPVSQGFGMSAA
                     GALSAALACADLLNLPRENAIKAAHYAEIQSHTGLGDVIASSFGGIEIRRKAGLPPWG
                     MLEHIPGNYDVVLCVIGKKIETKKILTDVTKVHEIASYGRYCTKKLLEKPSLEHLFSL
                     AWEFTRKIDIADTMVLQAIKKANAHGMASMCMLGNSVFAVGDIPVLCKILSIYGEVFC
                     CTVDEKGARLLQEK"
     gene            complement(1549..2745)
                     /gene="coaBC"
                     /locus_tag="VN377_02325"
     CDS             complement(1549..2745)
                     /gene="coaBC"
                     /locus_tag="VN377_02325"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWR27253.1"
                     /translation="MHPAEELRGKKTNKLLHKRIVLGITGSIAAVECVKLARDLIRHG
                     ATVYPVMTPSATRIIHPDAIEFATGHKPVIQLSGQTEHVSWCGLIQDPVDLLLISPCT
                     ANTISKIARGIDDTPVTTCATTAIGSGVPILLVPAMHLSMYDHKIVQKNIEICKDHGV
                     LFLNPVISGNKAKSPEVLEIVETVIRTIGKKDLKNKKILIIGGATAEPIDDIRVLTNR
                     SSGKTAVALCINAFERGADVELWYGYAQEAVPSYIPVKRFETVNDLLALTKDKGLKQV
                     DSVIVCAAIANYIPKKLKGKIPSGKETLAIDCFQAPVVLETLRSQIPHKKIIAFKTEE
                     KKQNVARRTQQLMTKYHLHSAIGNTLAGFGAEYNEILLLTKKGKSIWKKGKKEELASA
                     ILDMLR"
     gene            complement(2761..3201)
                     /locus_tag="VN377_02330"
     CDS             complement(2761..3201)
                     /locus_tag="VN377_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR27254.1"
                     /translation="MKRLLVAYDGSEASNKAIDLAVQCSTPDDELVLLTVIPAALVES
                     TFTNMLLPTIDLSTVVTPGTFKEKAMENLGKLAKELEGKISKVDVAVEAGDPADEILL
                     VAKKYDSDIILIGYKGYGKEGRFLLGSVTDKVVRHASRSVLVVR"
     gene            complement(3332..3841)
                     /locus_tag="VN377_02335"
     CDS             complement(3332..3841)
                     /locus_tag="VN377_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase"
                     /protein_id="HWR27255.1"
                     /translation="MSSAYERELKGILEGDEKILSKMTKTCSALEKGNYYLTKQKPFI
                     VVRAAGSFGVDLVALRGDISFLMEIKSSNADTLHFSSVDGKLQRQAEKMQRECEKTKT
                     LPVYGFRLKGHGGDCWRLFSMEISQLEGRAKILHNRLPKLSVSKNGNYIMRWEDGLPL
                     SDFLMYLCK"
     gene            3923..4477
                     /locus_tag="VN377_02340"
     CDS             3923..4477
                     /locus_tag="VN377_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27256.1"
                     /translation="MNKDVLVKEVMRIKPVVVQPFTTVLEAAQIMKQNKIGNVIISEV
                     NHPIGILTESDIIKKVVCEAKNAQDVLVEEVMSSPIIIAEPYISLQEALKIMGKCNIR
                     RLPLVENNQLVGIITQRDISRLSPALTEISHEWSSIDVKDEEYLQSQTFSGKCEDCNL
                     LSTSLKSIDGRLLCEDCIDGLKYE"
     gene            4508..5287
                     /locus_tag="VN377_02345"
     CDS             4508..5287
                     /locus_tag="VN377_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27257.1"
                     /translation="MKVKEIMTTKMVTVDKDDSLKLVLDLMKKHDITKIPVTEEKKFF
                     GLVTDNVIAYKLGSIRKRGITASRLHASSVTEKEVLMISPEEEVKNILNSVGEPGPTM
                     LPVVEKEKLVGIITKANLLPLVTSKNQLHSIMQKKVLTVGLEERVIHARRIMITNNVA
                     RLPVLEEKKLVGIISDIEIALAFASLKKSFSLGKQKHHLDELLVKDVMRSPVVWSNPS
                     MIISDAAQLMLKQNVGALPLLENDKLVGMVTRTDLLKTIVM"
     gene            5322..5711
                     /locus_tag="VN377_02350"
     CDS             5322..5711
                     /locus_tag="VN377_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27258.1"
                     /translation="MVYHIPSDEDILGAVEKVFQKSHTVTSQHTLKKLVEKELQLKKK
                     QFHVSEHRLRTLVLNTGLVQVEIRTREGDPDKILHKCPVCAGPLQRVKNQTIYGGEVT
                     IELRCDMCGYWTGKKKKIPTLYVFHLK"
     gene            complement(5722..6171)
                     /locus_tag="VN377_02355"
     CDS             complement(5722..6171)
                     /locus_tag="VN377_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27259.1"
                     /translation="MPVLKKSEIMKNVLRTLISISSRKTDLPYTIMTMEDLIRRLETQ
                     YRFLKHIQINDDFYNEQSGDMISVMSDINTIPPNELGKALHSIIDSMNRSLGENAGHF
                     FIKEIRNKLSDEYLTVMRDMGVDLGLMQLESEINRLEREIIERKKQS"
     gene            6359..6778
                     /locus_tag="VN377_02360"
     CDS             6359..6778
                     /locus_tag="VN377_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27260.1"
                     /translation="MKKVVFCVIAVVICISSALSGCQDTSSVKTQGKPQNVYLDSTIV
                     EFVNVSYEENINKSGGIESVTVGWLFHNIAGKLISAKIDVKFYDKNNLLLYNGTRSIN
                     NMPAGYTEHSFQPSNKVTYEGADVAFVDHVVISVTEK"
     gene            complement(6826..7368)
                     /locus_tag="VN377_02365"
     CDS             complement(6826..7368)
                     /locus_tag="VN377_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27261.1"
                     /translation="MKVKPILIYVLLVVNLSTFLFLSPFCSAQHANVSIKDVTVQLTQ
                     TRPPIGTTTIREYTITAILQNTGDSESVNITVKLKDPQPGLNATLIFQPEDSSLKPNE
                     EKTFVFENWPTSLSGDIALNISFQPSSPDVLLTEFNSGFYIYTLHIGDGTKTTSTPGF
                     EVLVVLIAILAFLFIKQIRK"
     gene            7531..7743
                     /locus_tag="VN377_02370"
     CDS             7531..7743
                     /locus_tag="VN377_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27262.1"
                     /translation="MLELRGIRKGIEVLVRNPDAPNLPARKAVVIHTYPRPSRWFVVR
                     YESGDVAQVEENQVTTMFEEYKKNID"
     gene            complement(7751..8131)
                     /locus_tag="VN377_02375"
     CDS             complement(7751..8131)
                     /locus_tag="VN377_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellum operon transcriptional activator EarA
                     family protein"
                     /protein_id="HWR27263.1"
                     /translation="MIPYIGFVDGMQDETKTAISPEMLKPLIIRSLRRSSVRKKIAEY
                     LFEISPSGSYTSEIAFSVKTTPTNVIGAIRGMNTRYRDDESLISLNLVEQIEGGKHRD
                     IKLYRLTDLGKEIVTGLRDNKKRF"
BASE COUNT         2486 a         1747 c         1717 g         2313 t
ORIGIN      
        1 catgggcggg agatggagga acctatgaca ttggcatcca agcactctcc ggcgccctcg
       61 aacggagaac caattttatt tacatctgtt ataacaatca aatctattcg aacaccggta
      121 ttcaacggag cggagcaacc ccgtatgccg cgtggacgac aacgacggtc agtgggaaaa
      181 atgagttccg aaaagaaatg ggggagatca tccttgcaca ccatatcccc tacgcagccc
      241 aaacctgtat cagttacccc gaggatgtga taaataaaat aacaaaagca aaaaacatac
      301 aaggtccgaa gtacatagaa atcctcgcac catgccctcc aggctggcga ttcagcatgg
      361 ataaaaccgt ggaaatggga agactcgcgg tcgactcagg cgcctggtcc ctgtgggaat
      421 gtgaaaacga tcagatgacg ttcaacggca aaagcaaact gattttagaa aaaaaggtcc
      481 aacgcaaacc agtggaagaa tggataaaat atcaaggacg gttcagccac cttttcaaac
      541 cacagcggga tgaggaacga ctcaaagcta tcgaaaacta cattgataat ctctgggatc
      601 gctaccgtaa gtgctacgca acgaagtaat gcaggaaaaa aacacagtca agaaaaaggg
      661 ttatttttct tgtaacaacc gtgctccttt ctcatcgacg gtacaacaaa aaacctcacc
      721 gtagatagaa agaatcttac agagaacggg gatgtctccg accgcaaata ccgagtttcc
      781 caacatacac atacttgcca tgccatgtgc gtttgctttc ttaatagctt gcagcaccat
      841 cgtatcagca atatcgattt ttcttgtgaa ttcccaggca agagaaaaaa gatgctcaag
      901 cgaaggcttt tccaataatt tttttgtgca atatcgtcca tacgatgcaa tctcgtggac
      961 cttggtcaca tccgtaagaa tcttttttgt ctcaatcttt ttcccaatca cacatagcac
     1021 gacatcatag ttccccggga tatgttccag catcccccag ggaggaagac ctgcttttcg
     1081 tcggatctca attccaccaa agctacttgc gatcacatca ccaagtcccg tgtgtgactg
     1141 gatttcagca taatgtgcag ccttgatcgc attttccctt ggaagattga gaagatctgc
     1201 acaagcgagg gctgcgctta acgctcctgc cgcgctcata ccaaatccct ggctcactgg
     1261 taaatccagg acagtattca ccgttacact caagggggta gtgcctatta gatagtacag
     1321 agcggatttt gtgactggcg cattcgatag ttttccactg atacgaacgg tgactgtccg
     1381 agaagacgca ggttgtattt tcacctggct gatcgcacca agcgtgatat taattccagc
     1441 gccacaggac ccagaccggt ctatattctg ctcagaatac accggttcaa aaaacgctga
     1501 aatatgccct ggagcaaaag cgattcctgt catagatatc ctgtttgact atcgtaacat
     1561 atcgaggata gcagatgcaa gctcttcttt ttttcctttt ttccagatgc tttttccttt
     1621 ttttgtgaga agaagaatct cgttatattc cgcaccaaac cccgcgaggg tattgccaat
     1681 agcactatgg agatggtact tggtcatcaa ctgctgtgtc ctcctagcga cgttttgttt
     1741 tttttcttcg gttttaaagg caatgatttt tttatgaggg atttgcgacc gcagggtctc
     1801 aagaacgacc ggtgcttgaa aacaatcgat agcgagtgtc tcttttccgg aggggatttt
     1861 ccctttcagc ttttttggga tatagtttgc aatcgctgca cagacgatga cgctgtcaac
     1921 ctgtttcagt cctttgtctt tcgtcaatgc aagaagatca ttgacggtct cgaatcgttt
     1981 aacaggaata taagacggaa cagcttcctg tgcataccca taccaaagct caacatcagc
     2041 tccccgttcg aatgcattta tacataatgc gaccgctgtc tttcctgaac tccgatttgt
     2101 aagcactcgg atatcatcga tgggttctgc tgtcgcaccg ccgataatca aaattttttt
     2161 gtttttcaaa tcttttttac ctatcgttcg aatcacggtt tcgacgatct ctagaacctc
     2221 tggggatttt gctttgttcc cagaaataac tggattgagg aacaacaccc catggtcttt
     2281 gcagatctcg atatttttct gaactatttt atgatcatac atcgacaggt gcatcgcagg
     2341 aacaagaaga atcggtacgc cagaacctat cgcggtggtc gcacaggtgg ttaccggtgt
     2401 atcatcaatt ccacgagcga tttttgaaat cgtgtttgcc gtacatggtg atatcaaaag
     2461 gaggtcaacg gggtcttgga ttaaaccaca ccaggagaca tgttccgtct ggccagataa
     2521 ttggataaca ggtttatgac cagtggcaaa ttcaatggca tccggatgaa taatgcgagt
     2581 tgctgacggg gtcatcacag gatacacggt cgctccatga cggatgagat cccgtgcgag
     2641 tttcacacat tcaaccgctg caatactacc agtaatccca agaacaatac gtttgtggag
     2701 aagtttgttt gttttcttcc cgcgtaattc ttcagcagga tgcatttcat cacgattctt
     2761 ctaccgtacg actagtacgg atctgctcgc atgtcgtact actttatctg tgacactgcc
     2821 tagtaaaaac cttccctctt ttccatatcc tttataccct atgagaataa tgtcactatc
     2881 atattttttt gccaccagca ggatttcatc tgcagggtcg ccagcttcaa cagcgacgtc
     2941 tactttgctg attttacctt caagttcttt tgcgagttta ccaaggttct ccatggcttt
     3001 ttctttaaaa gtcccagggg tcaccaccgt gctgagatca atggttggca gtagcatgtt
     3061 tgtgaatgtt gattcaacta acgcggcagg aatcaccgtg aggagaacaa gctcatcatc
     3121 aggtgttgaa cattggacgg ctaaatcaat tgctttattt gatgcttctg atccatcata
     3181 ggcgacaagt aatcgtttca taaatcatca cagtgctgct agtaataagg aactgactaa
     3241 aaaagatttt gtattacctc tcagaacgaa agagaaaaat tctaattaag aaccgttgtg
     3301 acgaagataa actctgatcc acgttattga actatttgca gagatacatg agaaaatctg
     3361 agagcggaag accatcttcc cagcgcataa tataatttcc gtttttactc acactcaact
     3421 ttgggagccg attgtgcaga atcttcgctc gcccctcaag ctgagaaatc tccatggaaa
     3481 agagccgcca acaatcacca ccatgacctt ttaaacgaaa accatagacc ggcaaggtct
     3541 ttgtcttttc gcattcacgt tgcatctttt ccgcctgtcg ttgcagtttc ccatcaacac
     3601 tactgaaatg caacgtatcg gcattactgg actttatctc cataagaaac gagatatccc
     3661 ctctgagagc aaccaaatcg acaccgaatg atcctgcagc acgaacgacg atgaacggtt
     3721 tctgtttcgt aagatagtaa tttcctttct ctaatgccga gcaggttttc gtcatttttg
     3781 aaagaatttt ttcatcgcct tcaagaatac ctttcaattc tcgttcgtaa gcgctgctca
     3841 tatgaaaaag gtaaaatacc tcgtaatgtt ttaatgtttg ggattggatc tttcaatcag
     3901 acagcgagtt ggtggataac ctatgaacaa agacgttcta gtcaaagagg tcatgaggat
     3961 aaaaccggtg gttgtacaac catttaccac cgttttagaa gcagctcaga taatgaaaca
     4021 gaacaaaata ggaaatgtga tcatctcaga agtaaaccac ccgatcggga ttcttacaga
     4081 aagcgatatt ataaaaaaag tggtgtgcga ggcgaaaaac gcacaggatg tgctcgtcga
     4141 agaagtcatg agcagcccga taattatcgc agaaccatac atctccttgc aagaggcgct
     4201 gaagattatg gggaaatgta atatcagaag actccccctc gttgaaaaca atcagcttgt
     4261 cggaattatc acacaacgtg atatctctcg gttatcacca gctttaactg agatttccca
     4321 tgagtggtca agcatagatg taaaagatga ggaatatcta caaagccaaa cattctctgg
     4381 aaaatgtgaa gattgcaatc ttttatccac aagcctcaaa agtattgatg gtcgcctgct
     4441 ctgcgaagac tgcatcgatg gattaaaata tgaataacat tttttaaaaa aaaggaggaa
     4501 aacggtgatg aaagtaaaag aaataatgac gacaaaaatg gttacagtag ataaagatga
     4561 tagtcttaaa ctcgtactgg acctgatgaa aaagcatgac atcacaaaga ttccagtgac
     4621 tgaagaaaag aaattctttg gtctggtaac cgacaacgtg attgcttata aactagggtc
     4681 gatacggaag cgaggaatca ccgcatcacg actccatgca tcatctgtga cagagaaaga
     4741 ggtcctgatg atttccccgg aagaggaagt aaaaaacata ttaaattcag ttggtgagcc
     4801 aggtccaacg atgctgccgg tagtcgaaaa agaaaagctt gtcgggataa taacaaaagc
     4861 gaatcttctc cctttggtca ccagcaaaaa tcaacttcat tcaatcatgc aaaaaaaagt
     4921 ccttaccgtc ggccttgaag agcgggtaat ccatgcacga cggattatga ttaccaataa
     4981 tgtcgcacgt ctcccagtcc ttgaggaaaa aaaactagtc ggaatcatct ctgatatcga
     5041 gatagcttta gcgtttgcat cgctaaaaaa atcgttttca ttaggtaaac agaaacatca
     5101 cctcgatgag ctccttgtta aggatgtcat gcgatccccg gttgtatggt ccaatccgtc
     5161 tatgattatt tctgatgcag cgcagttgat gctaaaacaa aatgtcggag cattaccctt
     5221 gttagaaaac gataagctcg tcgggatggt aacaaggacc gatttattaa agacaatcgt
     5281 gatgtaaaaa ggttaaagta agcaagacga ttcactattg tatggtgtac catattccct
     5341 ccgatgagga tatcctcggt gcggtggaaa aggtttttca gaagtctcat acagtgacgt
     5401 cacaacatac tcttaaaaaa ttagttgaaa aagaattaca attgaaaaaa aagcagttcc
     5461 atgtaagcga acaccggttg cggactcttg ttttgaatac cgggttggtt caggtcgaga
     5521 ttcggacaag agagggggac cctgataaga ttctgcataa atgccctgtc tgcgcaggcc
     5581 cattacagcg ggtgaaaaat cagaccatct atggaggtga agtcaccata gagttacgat
     5641 gtgatatgtg cggatactgg accggaaaga aaaagaaaat tccaacctta tacgtctttc
     5701 atctaaaata agaatggatt gttacgattg ttttttccgt tcaatgattt ctcgttccaa
     5761 cctgttgatt tcactctcta gttgcatcag acccagatcg acccccatat ctctcatcac
     5821 ggtgagatac tcatcgctaa gcttattgcg tatttccttt atgaaaaaat ggcctgcgtt
     5881 ctccccaaga gatcgattca tcgaatcaat aatggaatga agcgccttcc ctaactcatt
     5941 tggtggtatc gtattgatat ctgacatgac actgatcata tcaccagatt gttcattata
     6001 gaaatcgtca tttatttgaa tatgtttcag aaaacgatat tgagtttcta atcgccgtat
     6061 aagatcctcc atggtcataa tcgtataagg gagatctgtt tttctactag aaatcgatat
     6121 taacgtccgt aagacattct tcatgatttc tgattttttt agtaccggca tacccgttct
     6181 ctctgaatct acttccgttt cggttccttt tcccgattac tgaacctcaa tgatagtcca
     6241 tagatattaa aacttttggt gaaaaagact ttttttgaat actcatacac ttatatcgaa
     6301 acatataaat gtcgatgtac catatctttt ttaaggaaat caggagggac gagcacacat
     6361 gaagaaagta gtattttgtg ttatagctgt tgttatttgt atcagttcag cattaagtgg
     6421 ttgccaagac acaagcagcg tcaagacaca aggaaaacca caaaatgtgt atcttgattc
     6481 aaccattgtt gaatttgtca atgtgtctta tgaagagaat atcaataaat caggaggtat
     6541 agaatcagtg acagtcggat ggttatttca taacatcgca ggaaaactga ttagtgcaaa
     6601 gatagatgtt aaattttatg ataagaataa cctcctctta tataatggaa cgagatctat
     6661 caacaatatg cctgcagggt acaccgaaca ttcattccaa ccctcaaata aagtgaccta
     6721 tgagggagct gacgtagcat ttgtagatca cgtggtcatt tcagttactg aaaaataatc
     6781 ctgaatcaaa tattttcgtt tctattcgaa acagaatcag cgacgttatt ttcttatttg
     6841 ttttatgaat aagaaagcta gaatcgcaat aagaacgaca aggacttcaa aaccaggggt
     6901 cgaggtcgtt ttagtaccat ctccgatgtg tagtgtatag atataaaaac cagagttaaa
     6961 ttcggttagc agtacatctg gggatgaagg ttgaaagctg atattaagag caatgtcacc
     7021 ggataaagac gtcggccaat tttcgaagac aaaggttttc tcttcatttg gttttaatga
     7081 ggaatcctca ggttggaaaa tgagggtcgc attcagtcca ggttggggat ccttgagttt
     7141 gacagtgatg tttaccgact cggaatcacc ggtgttctgt aaaattgcgg ttatcgtata
     7201 ttctcgtatc gtcgtagtcc cgattggagg acgtgtttgt gttaattgta cagttacgtc
     7261 cttaatagag acgttcgcat gttgtgctga acagaacggg cttaaaaaca gaaaagtgct
     7321 caggttgaca acgagaagta catatatcaa tatcggtttt accttcatag gtctctcact
     7381 gtatcctgaa atcagatgca atatatataa acttcatcca ggaaaagcat tgtttttttt
     7441 ttatcaggac ctcttgatcg gttgaattac ttctttatac ttctaagata ttcgtttcga
     7501 tgaaaaaaaa ataaggctgg gaaatggtcg atgttggaac tacgcgggat acgaaagggt
     7561 attgaagttt tagtccgaaa tcctgacgct ccgaatctcc cagcccgaaa agcggtcgtc
     7621 attcatacgt atcctcgccc tagtcgatgg tttgtagtgc gatacgaaag tggcgatgtc
     7681 gcacaagtag aagaaaacca agtcacaacg atgttcgaag aatacaaaaa aaatattgat
     7741 tgatcttttt ttagaaacgc tttttgttgt ctcgaaggcc tgtcacgatt tctttgccta
     7801 aatcagtgag tctgtacaat ttaatatccc gatgcttccc cccttcgatt tgttcgacga
     7861 gatttaaact gataagagat tcatcatcac gatatcgcgt gttcatccca cgaatcgcac
     7921 cgatcacatt ggtcggagtg gtttttacgc tgaacgcaat ctcagaagta taactcccgc
     7981 tgggactgat ttcaaaaaga tactcagcta tttttttccg tacgctactt ctccgaagtg
     8041 atcgaatgat aagaggcttc aacatctctg gagaaatcgc tgtttttgtc tcatcctgca
     8101 tcccatccac gaaaccaata tagggaatca catggtgatt aataagctaa tcgattttct
     8161 atacaaaaaa aaatgggcta ggccggattc accaaccctg taaggagaga ttttgtgcga
     8221 ttgtttgcga aataacgacc aatctaacac ataagctcag ttc
//
LOCUS       DATMXJ010000082         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12111, whole genome shotgun sequence.
ACCESSION   DATMXJ010000082 DATMXJ010000000
VERSION     DATMXJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12111"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1335
                     /locus_tag="VN377_02380"
     CDS             124..1335
                     /locus_tag="VN377_02380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWR27264.1"
                     /translation="MKTTLVSLFLCSLVFTTSFSPAFIIPQTTSKTSQNTHFLPDMSF
                     DHLITVLLKIGHYPSLSACIIHNSTLVWKNSYGYADIEAHTKANTQTIYIVASITKTV
                     TSTALMQLYEQGLFDLDDDVNLYLPFPLRNPNYPDDPITIRMILSHSSSLVTDLMSYH
                     WFNYSNPPPIPWYPSPWLEEYLLPDGAYYTPAIWDATHRPGAYMQYANINFDLAAYLV
                     QLLSGQPFHEYCDDHIFTPLGMTHTSFRLPDLPIDDVAYPYLFQNWTYEKLPHYQLLH
                     YPIGGLMTTVDDLSCFLIAHMNHGVYNGTRILQNETVTLMHTIQPPGNIYENFHYGLG
                     WMIVEKPLRKKVYIGHSGDIPGTHTRMYIDQSDNTGIICFFNSDRSTYLKKFVSLLIQ
                     NILFAKAEQLT"
     gene            1394..2494
                     /locus_tag="VN377_02385"
     CDS             1394..2494
                     /locus_tag="VN377_02385"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWR27265.1"
                     /translation="MNVLLNLGDIINQLFRDNRININSFYGEAIAAVILFIGAILIGW
                     IVYHLFEHYFSRWAKKTKTTIDDEIIKNTKRPIYFFVIIVGFYYAIDQLSILKPYQFY
                     ITQIFLVAEVFLVAYIITRIINVILSWYAERIVKTGKKALSSNILLVFKKLLHVFVYI
                     FAFLYLLYVSHIDLSGAVVGLGVTGIAIAFALQSILGDAFSAFTIYFDRPFEIGDYVI
                     IGNYEGTVTNISMKSTRLKLLQGEELVISNKEITGGSLRNFKKMENRRVVFSIAITYD
                     TPPEKLKKIPALIKKIVETCDNVEFQRVHFKELGTFSLNFEIVYLLKSPDYVKYLDTQ
                     QTINYGIKEVFEKEKIDLAFPTQTILINQPRT"
     gene            complement(2495..>2875)
                     /locus_tag="VN377_02390"
     CDS             complement(2495..>2875)
                     /locus_tag="VN377_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27266.1"
                     /translation="RVFKEEQSITGTVNYVIGGLIPLGLQRPFLYRNILFVGDAGVGA
                     FPFSGQGIYRALMSGDIAGRCIAQDNIKRYPSIIRKEFTQWDVMGSIFIKMNLVFRKI
                     NPVLFIHSLNFFARQGKELKLLSH"
BASE COUNT          849 a          770 c          493 g          763 t
ORIGIN      
        1 gaggtagatc gaggtatttt acctacataa aaaatattca ttgatctttc atcttggaat
       61 tttctctttt cagaggaaaa cataatataa ttcttacctc attaataata tctaggaccc
      121 tttatgaaaa ccacactggt aagtctcttc ctctgttctc ttgttttcac aacaagcttt
      181 tccccagcat ttataatccc acagaccacg agtaaaacgt cacagaatac gcacttcctt
      241 cctgatatgt cctttgacca tcttatcacc gtgttactga agattggcca ttacccaagc
      301 ctgtccgcct gcatcatcca caattccacc ttggtctgga agaacagcta cggctatgct
      361 gacatcgagg cacataccaa ggcgaacact cagaccatct acatcgtcgc ctccattacc
      421 aaaaccgtca ccagcaccgc acttatgcaa ctctatgaac agggattatt tgaccttgat
      481 gacgacgtca acctctatct tccgtttcct ctcagaaacc cgaattatcc tgatgatcca
      541 atcacgatcc ggatgatcct ttcccattcc tccagcctcg tcacagatct catgtcatac
      601 cattggttca actactcaaa tccaccacct ataccctggt accccagccc atggcttgag
      661 gaatatctcc tcccagatgg tgcgtactac accccagcga tatgggatgc cacccatcgt
      721 ccaggggcat acatgcagta tgccaacatc aacttcgatc ttgcagcata tctggtacaa
      781 ctcctctcag gacaaccctt ccatgaatac tgcgacgacc atatcttcac tccccttgga
      841 atgacacaca caagttttcg cctaccagac ttacctatcg atgacgtcgc ctacccctat
      901 ctctttcaaa actggacgta cgaaaaactc ccccattacc aacttctcca ttatccgata
      961 ggggggttga tgacgactgt cgatgatctc tcctgcttcc tcatcgccca tatgaaccac
     1021 ggggtctata acggaacacg catcctgcag aacgagaccg tcacccttat gcacaccatc
     1081 cagccaccag ggaacatcta tgaaaatttt cattacggac ttggatggat gattgtcgaa
     1141 aaacctcttc gaaaaaaagt ctacatcggg catagcggcg atatccccgg gacacatact
     1201 cgcatgtata tagaccaatc agataacact ggcatcatct gtttcttcaa ctcagatcga
     1261 tccacgtatc taaaaaaatt cgtctccttg ctcatccaaa acatcctgtt cgccaaagca
     1321 gaacaactca cataaaaaaa cgaacataac attaaaaaat attcaatgga ttccccgcct
     1381 ggtgggatat cttatgaacg tccttctcaa cctaggtgac atcataaacc aactcttcag
     1441 agacaatcga ataaacatca actcctttta tggtgaagcc atcgcagcgg tgatcctctt
     1501 tattggtgct atactcattg gatggatcgt ctaccacctc ttcgagcatt acttcagcag
     1561 atgggcgaaa aaaacaaaaa ccaccatcga tgacgaaatc attaaaaaca ccaaacgacc
     1621 gatttacttt ttcgtcatca tcgttggatt ctactatgcg attgaccagc tctccatcct
     1681 caaaccatat caattttata tcacacaaat ctttctcgtt gcagaagtct tccttgtcgc
     1741 ctacatcatc accaggatca tcaacgtgat tctctcctgg tacgcagaac ggattgtaaa
     1801 aactgggaaa aaagcgttaa gcagcaacat cctgcttgtg tttaaaaaat tactacatgt
     1861 gttcgtctat attttcgcat tcctttatct tctctatgtc agccatatag atttatcagg
     1921 tgcggtcgtc ggactcggag tcaccgggat tgcaatcgca tttgcgctgc aaagtattct
     1981 tggagatgcg ttcagtgctt ttacgatcta cttcgaccga ccgtttgaaa tcggggatta
     2041 cgtcatcatc ggtaattacg aaggcaccgt caccaacatc agtatgaaat ccacacgcct
     2101 caaactcctt caaggagaag agctcgtcat ctccaacaag gaaatcactg gcggaagtct
     2161 tcgtaacttt aagaaaatgg agaaccgcag ggtcgtcttc tccatcgcaa tcacctatga
     2221 cacccctccg gagaaactaa agaagattcc ggcgcttatt aaaaaaatcg ttgaaacatg
     2281 cgacaatgta gagttccaac gggttcattt taaggaactg gggacattta gcctcaactt
     2341 cgagatcgtc tatcttttga aaagtccaga ttatgttaaa tatcttgaca cccagcaaac
     2401 catcaattac ggtatcaaag aagtctttga gaaagaaaaa atcgatcttg ccttcccaac
     2461 acaaactatt ctgattaatc aaccccgtac gtaatcagtg ggagagtaac tttaattctt
     2521 tcccctgtct ggcaaagaaa ttcaacgaat gaataaacaa cacaggatta atttttcgaa
     2581 aaaccaaatt cattttgata aatatggatc ccatcacatc ccattgtgta aactccttcc
     2641 ggatgataga cggatatctt tttatgttgt cctgcgcgat gcatcgacca gcgatatcac
     2701 cgctcatcaa cgctcggtag attccctgac ctgagaatgg aaacgcgccc acacctgcat
     2761 ccccaacaaa gagaatgttc ctgtagagga aaggacgttg caaaccaagc gggatcaatc
     2821 caccgatcac atagttcacg gtgcctgtga tggattgctc ttctttgaaa acacg
//
LOCUS       DATMXJ010000083         4550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_48783, whole genome shotgun sequence.
ACCESSION   DATMXJ010000083 DATMXJ010000000
VERSION     DATMXJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48783"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..691)
                     /locus_tag="VN377_02395"
     CDS             complement(167..691)
                     /locus_tag="VN377_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HWR27267.1"
                     /translation="MKKILLIGITAALIFATVFSGCLQQGTGTLILQITDAPGDLNIT
                     HANVTISQVQVHMSAGGGNNSTAGWYTIVNESQTFDLVALTNVTEFFGSANLSVGMYT
                     QIRLTVESCVITVNGTKYDCTVPSGAIKLIKPFVLKANETTILTLDFDAQESISETEN
                     HSYKFHPVIKVIQE"
     gene            complement(795..1145)
                     /locus_tag="VN377_02400"
     CDS             complement(795..1145)
                     /locus_tag="VN377_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27268.1"
                     /translation="MTKTSLGLEENFEAVLCYLFFWVSGLFFYFVEDKNKFIRFHAMQ
                     SILVFLPLMILAWIFGGFFGLFSYGAGWYFFAWISWIFWALVLIMWLILMVKAYQMTK
                     YKLPIVGDIAEKNS"
     gene            complement(1264..2742)
                     /locus_tag="VN377_02405"
     CDS             complement(1264..2742)
                     /locus_tag="VN377_02405"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27269.1"
                     /translation="MAFKKKKTSFMIALLFLLTAFVGMQTSGTALEAVLVAKAGGPYL
                     GEECSAILLNASGSYDMDGDPLTYRWNIDGSWIENGNYPYMEWTWYDDYSGEIILEVS
                     DGTNTSTDSAFVTISNVPPQIFSIDASTEVDVDTEFFLSVNFFDGLPDPRSPIASLDT
                     FTVTFSWDDGTSTGLSLGAEEFSASASHLFAEAGIYQISIIIVDSDGGEAVTEWDVTV
                     GEYASVEAGPDVLINEGSMFLSHGFLADTDSLLFSAIVDYYDDTGPQPLSLNPGNTFD
                     LRHTYVDDGVYNLLVTAFNDEMEYGSDTAIVTVNNVPPSVESLSVSPTSPYLPGDLFQ
                     LSALFSDPGILDTHTVKIEWGDGSSTQNAAEASITKISGSHSYVKAGEYKIVVTVTDD
                     DGGTADASISVVVKSPTTSTDAIREIIIGLKIPKGLKNNLLSILENIPHLLKHHKLHA
                     VIHQLQAFIHYVEAQYGKSLPRAQAKTLIHTAQSVIDTLRIR"
     gene            complement(2893..>4550)
                     /locus_tag="VN377_02410"
     CDS             complement(2893..>4550)
                     /locus_tag="VN377_02410"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27270.1"
                     /translation="KKRVICIGMMLVLTAFVGITTNVSAGGLPPVADAGGPYTGVECD
                     SMLLDASGSYDPDGDPLTYRWFIDGSWYDSAGPNYDWIWMDDFTGVITLEVSDGDLTD
                     TDTADVTVSNVPPVINSTVGPTEVELGTEVPLAVNFFDGLFDPRGFIASLDTYTATFY
                     WDDGTTTTLVLGVEEFWANASHMYTEPGLYFIIIAIIDDNGGEADAIWEVSVNLKFVD
                     AGQDAFINEGSMFVSSGFLADDSGTYTAQVDYDDGTGMQALVLNPGNSFDLSHQYGDN
                     GVYSVVVTAFTEDGEDLGYDSATVTVYNVAPTIESLIGSSADPVQLGTPILLNGVFSD
                     PGYLDTHIATIDWGDGQTTSVNVPFGSHEVAGSHLYASAGVYQITLTVTDDDGGSDSE
                     TLQYYVVIYDPSAGFVTGGGWIIAQPGSYPANPSLTGKATFGFVSKYKKGQNQPEGNT
                     EFQFHAAGMNFHSHTYEWLVVAGSKAMYKGVGTINGAGNYGFKIIVIDGQKNGGGGID
                     KFRMKIWDLDNNDAIVFDNNLGLSDDENPVTALSGGQITIHKN"
BASE COUNT         1332 a         1022 c          925 g         1271 t
ORIGIN      
        1 tctttgcgat tctgtaggtg gattgttctc ctttgtctat ttgtgtgatg atccatcgta
       61 ttttctgttg attcagtttc ctcatcggaa ctgaatcatc attgtttttt tctaggagga
      121 agtaatttcg ggaaaagaca acaaaaaaaa aaaagggaaa aagtgattat tcttgtatca
      181 ctttgatgac tgggtggaac ttgtaggaat gattttctgt ttctgagatc gactcctggg
      241 catcaaaatc caacgtcaag attgtcgtct catttgcttt taacacgaat ggcttgatga
      301 gtttgattgc tccagaggga accgtacaat catatttcgt cccattcaca gtgatgacac
      361 aggactcaac ggtgagtcgt atctgcgtgt acatcccgac gctaaggttc gcactaccga
      421 aaaactctgt gacgtttgtc agagcaacca gatcaaaggt ctgggactca ttgacaatcg
      481 tgtaccaccc agcggtcgaa ttattccccc caccagcgct catgtgcacc tggacctggg
      541 agatggtgac atttgcatgg gtgatgttca gatcaccagg agcgtctgta atctgtagta
      601 tcaatgtccc ggttccttgt tgtaaacatc cactaaacac tgttgcaaag atcaatgctg
      661 ctgttattcc aatcaataag atttttttca tttctgaact tccttatttt ttctatatat
      721 taattcgttt ataaagtctt ctacgcaata tgttagaaaa agtatgaaat gatttgaaag
      781 ctgaattgtt acgattatga gtttttctcg gcgatgtcac caacaatcgg aagcttatat
      841 ttcgtcatct gataggcttt gaccatcagg ataagccaca tgattaacac cagtgcccag
      901 aaaatccagc tgatccaggc gaagaaatac catccagccc catacgaaaa taatccgaaa
      961 aatcctccga aaatccaggc aaggatcatt aacggaagaa acactaggat tgattgcatt
     1021 gcgtggaatc ggatgaactt attcttatct tcgacaaaat agaagaacag cccagagacc
     1081 caaaagaaaa gatagcaaag cacagcctca aaattctcct ctaaccctaa cgacgttttc
     1141 gtcataaccc tataacgatg aaagccttat tagcattatc cataaaatac tgtgatgaag
     1201 aggtttggta taaaaaacgg tcaatatgga aaaaaaagaa gaaaaaatca gaagtgattt
     1261 ttgttacctt attcttaacg tatctatgac cgattgagca gtatgaatca gtgttttcgc
     1321 ttgagctctg gggaggcttt tcccgtattg cgcttcgacg tagtgaatga acgcttggag
     1381 ttgatgaatc accgcatgta gtttatgatg tttgagaaga tgtggaatgt tctccaggat
     1441 tgataaaaga ttattcttca ggccttttgg gatttttaga ccaataatga tttctctgat
     1501 ggcatctgtc gaggtcgtag ggcttttcac aacaacagat atcgatgcat cagcagtacc
     1561 gccatcatca tcagtgacag tcacgactat tttgtattca cctgctttta catagctatg
     1621 agagccactt attttggtga tacttgcttc agcggcgttc tgtgttgagg aaccatcccc
     1681 ccattcaatc tttacagtgt gagtgtcaag aattcctgga tcggagaaaa gagcactgag
     1741 ttgaaacaga tcaccaggca ggtaaggact cgttggtgac accgacagcg attcaacaga
     1801 tggaggaaca ttattgaccg ttactatcgc ggtgtctgat ccgtattcca tctcatcatt
     1861 gaacgccgtg acgagaaggt tgtagacgcc gtcatcgaca taggtgtgac gtaaatcaaa
     1921 ggtatttcca gggttaagcg aaagtggctg aggccctgta tcatcatagt aatcaacaat
     1981 ggcgctgaac agaagagaat ccgtgtctgc aaggaaacca tggcttagaa acattgatcc
     2041 ttcattgatg agaacgtccg gtccggcttc gactgatgca tactcgccga ccgtaacatc
     2101 ccattcagtg actgcttccc cgccatcaga atcaacaata ataatggaga tttgatagat
     2161 ccccgcttct gcaaacagat gagacgcgct agctgaaaat tcttcagccc ctaaagaaag
     2221 accggttgag gttccatcat cccaagaaaa cgttacagtg aacgtatcca aggacgctat
     2281 tggagaccgt ggatctggca atccatcaaa aaaattcacc gataaaaaaa actctgtgtc
     2341 cacatctacc tcggtcgatg catcaatgga aaaaatctgc ggtggtacgt tggatatcgt
     2401 tacgaacgca ctatccgttg aggtgttcgt tccatcagag acttcgagga tgatttcacc
     2461 ggaataatca tcataccagg tccattccat atacggatag ttcccattct ctatccatga
     2521 tccgtcgata ttccagcgat aggtcaaagg atcaccatcc atgtcatagg atcctgatgc
     2581 attgagcaat atcgcactac attcctcacc cagatacgga ccacctgctt tagcaactaa
     2641 gactgcttcc aaagcagtac cagatgtctg cataccaaca aaggcagtca atagaaataa
     2701 cagtgcgatc ataaaactgg tttttttctt tttaaaggcc atcgtgtcta cctccaccca
     2761 ccatatatta ttttgtcgtg gaaagtatat aaaaatattc tatgagaatg acaaaacaag
     2821 cagaaataca aaatggaaaa atatcaaaaa aaaatctaaa aaaaaaagaa aaaataggtg
     2881 gatgagttct atctagttct tatggattgt tatctgtcca ccactcaatg ccgtgaccgg
     2941 gttctcgtcg tcagataatc caaggttgtt gtcaaaaacg attgcatcat tgttgtccaa
     3001 atcccaaatt ttcattctga atttgtcgat gccaccgcct ccgtttttct gtccgtctat
     3061 gacgataatc ttgaatccat agttgcctgc tccgttgatt gtcccgactc ctttatacat
     3121 cgcttttgaa ccagccacga ctaaccattc gtaggtgtgc gaatggaaat tcatgccagc
     3181 tgcatggaac tggaattcgg tattgccttc aggttgattc tgtcccttct tgtattttga
     3241 aacaaaacca aaagttgctt taccggtaag agacggattc gcaggatagg accctggttg
     3301 ggcaatgatc catccaccac cggtgacgaa tccagcgctt ggatcatata tcacgacata
     3361 atattgtaat gtctcagagt cagatccgcc atcatcatca gtcacggtga gagtaatctg
     3421 atacacacca gcactggcgt acaggtggct tcccgcaact tcgtgggagc caaatggtac
     3481 gttgacgctt gttgtttggc catctcccca gtcgattgtt gcgatatggg tgtcaagata
     3541 accagggtca gagaacacac cgtttaagag aattggagta ccgagttgta ctggatcggc
     3601 tgatgaccca atcaatgatt caatggtggg tgcgacgtta tatactgtta ctgtcgcact
     3661 atcatatcct agatcttcac catcttcagt gaatgcagta acaacaacag agtaaacccc
     3721 attatcacca tattgatgtg ataaatcaaa gctgtttcct gggttcaaga caagcgcttg
     3781 catccctgtt ccgtcatcgt aatcaacctg tgcggtatat gtgccgctat catcagcgag
     3841 aaaaccagaa cttacgaaca ttgacccttc attgatgaaa gcatcctgtc cggcatccac
     3901 aaatttgaga ttgacactga cttcccatat ggcatctgct tctccgccat tatcatcgat
     3961 aatggcaatg ataatgaaat aaagaccagg ctctgtgtac atgtgagaag cattcgccca
     4021 aaactcttct acccctaaaa ctaacgttgt cgtggttcca tcatcccagt aaaacgttgc
     4081 tgtatacgta tctaatgaag ctatgaaacc ccggggatcg aacagtccat caaaaaaatt
     4141 cacagctaaa ggaacctctg ttcctaattc aacttctgtg ggtccgactg tggaattaat
     4201 gacgggggga acgtttgata ctgtgacatc tgcggtatct gtatctgtca aatctccatc
     4261 tgaaacttcg agggttatta cacctgtaaa gtcatccatc catatccaat cataattcgg
     4321 tcctgcactg tcataccaag atccatcgat gaaccatcga tacgttaatg gatcgccgtc
     4381 cgggtcatat gagcctgatg catctaatag catggaatca cattccacac cagtatatgg
     4441 tccacctgca tcagcaactg gtggtaatcc tcccgcactt acatttgtgg taatccccac
     4501 aaatgccgta agcaccagca tcatacctat acaaattact ctttttttcc
//
LOCUS       DATMXJ010000084         9997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_32970, whole genome shotgun sequence.
ACCESSION   DATMXJ010000084 DATMXJ010000000
VERSION     DATMXJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32970"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VN377_02415"
     CDS             <1..518
                     /locus_tag="VN377_02415"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27271.1"
                     /translation="SFVMKKLEKQGKNFDFGERGGIATGGGWKAYEGARVTVADFRKQ
                     VEKVLGIPEKYCLDIYGMVEGNGWLVHCPEGHYLHSPYSYYKSLVLDDELKPIGYGEW
                     GRYAFLDAAAYSYPGFITSGDKVRMLEHCPVCDRPGPVLEPEIKRASGAEVRGCAEEV
                     RRMLSADLSKE"
     gene            523..1506
                     /locus_tag="VN377_02420"
     CDS             523..1506
                     /locus_tag="VN377_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWR27272.1"
                     /translation="MLTPWEIKRKEMIPTRHMLQMNIGGVFFVLKMLLSNLGTFKMLK
                     NPTTKNVYIRPTPTYTIPPYSPEMKSCNSEEKYLRPTLFCNCRAPEIIALANQLGAYQ
                     KTDKECAEDAFEFAKRKIILEQTPLDDVTTIVHRGTGTCLHKISVFIALCRAAGIPAR
                     YKFFALTLLDEFVAPSLDRAPLIKQWSEAMGYFLVHGAGEVYIDGKWITADVGAEPTR
                     QASTWMPITKLGEDGAGLWLFPIPGSTIIRESIPLGLGIPSRILMHHLVPNSVAGLNI
                     GILEQIEKGKKIIQEAGGEAAYDAEARSRRRQASIKDELLKKHESIVFETN"
     gene            1702..2679
                     /locus_tag="VN377_02425"
     CDS             1702..2679
                     /locus_tag="VN377_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWR27273.1"
                     /translation="MLSFREMKDREYIPHGTYLKLLIGGSLSLIKVLLTNPGDLKKLR
                     TIQGSEERYIRPKRPYELPLYKEGMQCGVTDEKYLRPTLYCNPRAPEVVALAHHLGAF
                     QKTDYEFAKAAFEFVKEKLDLEICQMDPVEETIRRGTGTCFHLISVFIALCRCAGIKA
                     RYKTFAMNMIQTWYDAMVGSDPLVKKWYDQMGFFMMEGEGEAFIDGKWIPAHVGPTAE
                     RQAAGGIPITKFGEGSLGIWFFALPGTTMNMESIPYGLGAGSKLLKRIAPASMERVNI
                     SIQHQNITGKKIIEDAGGKETYDAMARKKRASKAPTLDLSDKKGIVFKE"
     gene            2687..3961
                     /locus_tag="VN377_02430"
     CDS             2687..3961
                     /locus_tag="VN377_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR27274.1"
                     /translation="MVSGEEKTSSSLKNQRTRRYILYLVIFMGMIAIMDQYLSFMETV
                     AIPEILKEYNISASQFSWWEALYFIPTFFIFLLNGLTDIIGRKLSILILFTMMGFSSL
                     AIVFLTPSFHFFMLFLAIITFTTVSNMWTIPISEEAPAEKRAKLVSIVYGISLLPIAA
                     ILPLLIDHFSLGWRWYYGTMFFFMIPVFIMWIFMKETYRYEIIKEERRRGVRKRHFFG
                     LGVINRCDMRYILFSAVIWMCWLIVSLIGGKYIGYYFVTVNLYPQASWLLIFLGILLL
                     MMVGSFVGGWTMDKIGRKKGLIIGCIGLGFFMGALGVVPFSMAKFIAPVTGFFLGFSY
                     TWIIVYITEIFPTERRGSCIGWTTTTARGSYVIGPIVAAILLDRYPTMDWFWVITGLL
                     MLIPIILVFLFHPFETRRKELEEIEEKRQSSS"
     gene            4177..4332
                     /locus_tag="VN377_02435"
     CDS             4177..4332
                     /locus_tag="VN377_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27275.1"
                     /translation="MKTILRRKDNTSIFCPTIQQKYPPSPKTKGIELMIACFFIVIGL
                     CIIIIMQ"
     gene            4452..4748
                     /locus_tag="VN377_02440"
     CDS             4452..4748
                     /locus_tag="VN377_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010478789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HWR27276.1"
                     /translation="MISVKVYRQGDDVVIGACDEHLLGKKFRKGKLQIDVAKRFYDGE
                     RIDKKTLETFLRDATIANLVGRETVACAIDLGLVDPQCILKIKGVPHAQIVQMI"
     gene            complement(4752..5222)
                     /locus_tag="VN377_02445"
     CDS             complement(4752..5222)
                     /locus_tag="VN377_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HWR27277.1"
                     /translation="MKLFCDHMLGSLAKWLRIFGFDTVYPDATMNDDIVLQIAKREKR
                     LLISRDNELLIRAKKALVPVLEIHTTDLTEQLQLVLKETTLDEEKILTRCTLCNTPLH
                     SIAKEAIKDQIPEKVFQTRNEFWFCPSCSKYYWMGTHYENMREKINTLIKKESL"
     gene            complement(5283..7082)
                     /locus_tag="VN377_02450"
     CDS             complement(5283..7082)
                     /locus_tag="VN377_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27278.1"
                     /translation="MNITHHKKSIFQALFTFVIAGLFIIPGSASVFTELQKNSEQTVG
                     RGSTIYVDDNNTAGPWDGTIDHPYRYIQDGIDDAIGGDIVSVFNGIYKENIVVSKSLE
                     IIGEDKENTVITGNDFGTVVKIIAEGVTITGFTITRSGSNPNNAGIMIHTPYNTIINN
                     NIQKNNYYGIYVIEGYDNTIYHNNFVENTYQAFDVIASSAWDGGYPTGGNYWDDYNSS
                     DENEDGIGDIPYPTGNSSVDQYPLIHPYGSIRNADTDEIFLSIQAAIDDDNTQNGHLI
                     VISVGVYWEHLCITKSLILHGVHGDTSIIDGRYSGDVITISADNVNLIGFMIQHSGTA
                     EQNAGIAVNGNNCSIEQNTIYDNFQGIILKQSTKGTQIIDNKISGQGWNGVTLKPGCT
                     GNYILENTIIDSFYAGIGISESSNNYIYHNNFKSNRHQAYDNAANIWDDGYPSGGNYW
                     SDYTGSDSDGDGIGDISYAIPDGINTDQYPLITPYTGGDTTPPIVKIQTPSNGLYLWG
                     LRLFSGLFRKSTIIYGPITIQVEAADAQSDIKQVEFLIDDSVTPAFVDTQAPYSWVWS
                     TPSLLMHKHTIIVIAYDNAGNPNYDMLEVRKYL"
     gene            complement(7191..8297)
                     /locus_tag="VN377_02455"
     CDS             complement(7191..8297)
                     /locus_tag="VN377_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWR27279.1"
                     /translation="MIDKRLEKLATILTRYSLNLKKNDLFVISGSHLAAPLIKEVYYQ
                     AVQIGAHPFTHIGIDGLAEIYYQNSSDQQLKYVSPLSKYEIEQIDASLSIISPENTRN
                     MTNIDPKKQAISNVAHHQIHEIFMNRAAKKELRWCITQYPTQASAQDAEMSLEDYENF
                     VFNAAHVQAKDPIKYWRTVQSDQEKIRTFLNKKKTLHVIGTDTDLRISVAGRKWINCS
                     GTENFPDGEVFTGPVETSADGHISYSFPGSYNGREVNDIQLWFKKGVVVKATASKGEQ
                     FLHTMLAMDPGAKRLGEFAFGTNYGVKNYTKNTLFDEKIGGTIHLAVGSGYSETGSKN
                     KSSLHWDMVCDLRKNGEIYADDELIYKNGRFLNI"
     gene            complement(8352..9605)
                     /locus_tag="VN377_02460"
     CDS             complement(8352..9605)
                     /locus_tag="VN377_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR27280.1"
                     /translation="MIIRKKYLFYVLAITSSFIAAAVTGVDSFVGGQPAFKTDPWSFA
                     FSLFFIGVVITLLITLALSVKIKGQSIGAKILDPSFKQIRMVQKSEMKYHSIAGLVNA
                     INTVGYCAIISAVQDPSIVLSFSQIVILYLLLMESITEKDVPTLVEIQSSVIVTFGAI
                     LASISLTGEFQLFPILIVFLVVNPTWAIFSIYQRKLKIMRINNRPNDSINIRFWNVIF
                     SCLFTALLLFIYDIITSGNHLLSGITAAIDPFYFGLLCLTMGTTFFSYVLYIRSLGMG
                     KASVNNAIRASTIIFAIPFSIILLQLGTITEFSTDPVMLIIKVIGMVLIVMGIISFAL
                     TQVKAYIFIDVKTGRPLKETLQKLWDIRGVSHVTITSGKHDFIVKVSTRTLIKGYERI
                     IRKLDEIEAIKKYHWESVLKDWEKI"
     gene            complement(9618..9860)
                     /locus_tag="VN377_02465"
     CDS             complement(9618..9860)
                     /locus_tag="VN377_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR27281.1"
                     /translation="MISAYILITMQPGKTDKAIKEMRQLDYVAKISIIAGEYDIVVRA
                     QVKNLEELLDVTDKIQMIEGVTKTTTQIIEKEISLQ"
BASE COUNT         2727 a         2119 c         2263 g         2888 t
ORIGIN      
        1 tttcgtttgt gatgaaaaag ctggaaaaac aaggaaaaaa cttcgatttt ggggaacgtg
       61 gaggcatcgc aactggcggg ggatggaaag catacgaagg cgcccgcgtt accgtggcag
      121 acttccgcaa acaagtcgaa aaagtccttg gcatccctga gaaatactgc ctggatatct
      181 atgggatggt cgaaggaaac ggctggctgg tccattgtcc tgaaggacac tacctccatt
      241 caccctactc atactacaaa tctcttgtcc tcgatgatga gttaaaacca atcgggtacg
      301 gtgaatgggg acgctatgcg ttccttgatg ccgccgcgta cagctacccc ggattcatca
      361 cttcaggaga taaagtccga atgctcgaac attgcccggt ctgtgaccgt cccggcccag
      421 tacttgaacc agagatcaaa cgagcatcag gcgcggaagt ccgagggtgc gctgaggaag
      481 tcagacggat gctttccgct gatctcagca aggaataata gcatgttgac cccctgggag
      541 atcaaacgaa aagaaatgat ccccacacgc catatgctcc agatgaacat cgggggtgtt
      601 ttttttgttt tgaagatgtt attgtcgaat ctcggcacct ttaaaatgtt aaagaaccca
      661 acaacaaaaa atgtgtacat ccgaccaaca cccacctata caatcccacc gtactcccct
      721 gaaatgaaga gctgcaactc agaagaaaaa tacctcagac caacattgtt ttgtaactgc
      781 cgagcaccag agatcatcgc attggccaat caactcgggg cgtaccaaaa gacagacaag
      841 gagtgtgcgg aggatgcctt tgagtttgcg aaaagaaaaa taatccttga gcaaaccccc
      901 ctggacgatg tcacaacgat tgtacatcga gggaccggga cttgtctgca taaaatttca
      961 gtatttatcg cattatgtcg tgctgctggg atccctgccc gatacaagtt tttcgccctc
     1021 accctccttg acgaattcgt ggcaccatcc cttgatcggg caccacttat aaagcagtgg
     1081 tccgaggcca tgggatattt cttggtccat ggcgcaggtg aagtgtatat cgatgggaaa
     1141 tggatcaccg cggacgttgg agcggaacca acacgtcaag catcaacctg gatgcctatc
     1201 acaaaactag gggaagacgg cgcaggactc tggttattcc caatcccagg gtccaccatc
     1261 atcagggaat ccatccctct tgggcttggg attccctcga gaatcctgat gcaccatctt
     1321 gtaccaaact ccgttgctgg actcaatatc gggattttag aacaaattga gaaaggtaaa
     1381 aaaatcatcc aggaagcagg cggagaagca gcctatgacg cagaggcacg aagcagacgc
     1441 aggcaggcat cgataaaaga tgagctgtta aaaaaacatg aaagtattgt ctttgagact
     1501 aattaaatgc aaaaaaccgt tcgtagttcg tcgtaatact cgtaggtcat atccttgttc
     1561 tataagtcct cgacgagatg actgccgata aaacctgttc cacctgtcac cacagcctgc
     1621 atagaaaaag agaatacctt ttttcctaaa aaaccatttg gaaaagataa tatgcccctt
     1681 gtgtttctgg gagatacttg tatgctgagt ttccgagaga tgaaggatag ggaatacatt
     1741 ccacatggaa cctatctgaa actgctgatt ggcggtagtc tttctttgat taaggtgtta
     1801 ctcaccaacc caggtgattt gaaaaaactc cggacaatcc agggttccga ggaacgatat
     1861 atcagaccaa aacggcccta tgaactaccg ctttataagg aagggatgca atgtggcgtc
     1921 actgatgaga aatacctcag accgacactg tattgtaacc cgcgcgctcc tgaggttgtc
     1981 gctcttgctc atcaccttgg tgcatttcag aaaacagact atgagttcgc aaaagctgct
     2041 tttgaattcg tcaaagaaaa gcttgattta gaaatttgcc agatggatcc tgtcgaggag
     2101 actatacgcc ggggtaccgg aacctgtttt catttaatct cggtgttcat agcattgtgt
     2161 cgttgcgctg ggatcaaggc acggtataag acgtttgcga tgaacatgat tcaaacatgg
     2221 tacgatgcta tggttgggtc ggatccattg gtaaaaaaat ggtacgatca aatgggattc
     2281 tttatgatgg aaggagaagg agaggcattc attgatggaa aatggatccc cgctcatgtc
     2341 ggccccaccg cagaacgaca agcagcaggc ggcatcccaa ttacgaagtt tggtgagggt
     2401 tctcttggca tctggttctt tgcactgcct ggaacaacta tgaatatgga atcgatcccg
     2461 tatggtttag gtgcagggtc caaactttta aaaaggatcg ctcccgcttc tatggaacgc
     2521 gtcaacatca gcattcaaca tcaaaacata acgggaaaaa aaatcataga agatgcaggc
     2581 ggtaaagaaa cctatgatgc aatggcacgc aaaaaacgcg catcgaaagc accaactctt
     2641 gatttatccg ataaaaaagg gatagtattc aaggagtgaa acaggcatgg tttctggtga
     2701 ggagaaaaca agcagtagtc taaagaatca gcgtactcgt cgttatattc tttatcttgt
     2761 cattttcatg ggtatgatcg caatcatgga tcaatacctc tcatttatgg agactgtcgc
     2821 catcccagaa attttgaaag agtataacat cagcgcatcc cagttctcat ggtgggaggc
     2881 actctatttc atcccgacgt ttttcatctt tcttctcaat ggcttaacag atattattgg
     2941 acgaaaatta tctattctca ttctattcac tatgatggga ttttcatctc ttgctatcgt
     3001 tttcttgacc ccttcatttc acttctttat gttgttcttg gcaatcatca catttaccac
     3061 tgtctcgaac atgtggacga ttccaatcag cgaagaagca ccagcggaaa aaagagccaa
     3121 actcgtctct attgtatatg gtattagtct tctgcctata gctgcaattc tcccgcttct
     3181 tattgatcat tttagccttg gctggcgatg gtactatgga actatgttct ttttcatgat
     3241 accggtattt atcatgtgga ttttcatgaa agaaacatat cgatacgaaa tcattaagga
     3301 ggaacgacgg cgaggcgttc ggaaacgaca tttttttgga cttggggtta ttaaccgctg
     3361 cgatatgaga tacattttgt tctcagcagt tatttggatg tgttggctaa ttgtctccct
     3421 gatcgggggg aagtacattg ggtactattt cgtcactgta aacctatacc ctcaagcatc
     3481 atggttgctg atattccttg gaatccttct gctgatgatg gtcggttcat ttgtcggcgg
     3541 ttggactatg gacaaaattg ggagaaagaa aggactcatc attggttgta tcggacttgg
     3601 attcttcatg ggtgcacttg gggtcgttcc tttttcaatg gccaaattca tcgctccggt
     3661 gacaggattc ttccttggat tttcatacac ctggatcatc gtttatatta cagagatttt
     3721 tccaacggaa agacgtggta gctgcatcgg atggactaca acaactgctc gaggctcata
     3781 tgtcatcgga ccgatagtgg ccgctattct ccttgatcgt tatcccacca tggattggtt
     3841 ctgggtaatt actggactcc tgatgttaat acctattatc cttgtgttcc tatttcatcc
     3901 gttcgaaacc agaagaaaag aattggaaga aatcgaggaa aaaagacaat cgagttcata
     3961 aagcagcaca taactcgacc attcttcgga tattttctca gtagaatctc ctcgaaaaga
     4021 aatcataact ttttctcaga acgcttctgc gcggtaattc acagacacgt acctctggta
     4081 aaagacttca aaaaaagatg atatcgaaat cggtacgggt tgtacaacaa gtcctttata
     4141 acgcgagcga ccacagtatt agctgaggaa agccttatga aaacaattct ccggagaaaa
     4201 gacaatacca gtattttctg tccaaccata caacaaaaat accccccctc ccctaaaacg
     4261 aaaggaattg aattgatgat tgcgtgcttc ttcattgtaa tcggactgtg tattatcatc
     4321 atcatgcagt aagcaaaaga tcaattctct cttatatcaa tccctttatt ttttccccct
     4381 tttttcgaaa tttatataaa acagaagttc ctaatagaca cttaacgaca gtgaacaaag
     4441 gacgtacacg catgatcagt gttaaagtct accgacaagg tgatgacgtt gtcatcggtg
     4501 cctgcgacga acatctcctt ggcaagaagt tccgcaaagg aaaactccaa attgatgtag
     4561 cgaaacgctt ctatgatgga gaacgaatcg ataagaaaac cttggaaacg tttctacgtg
     4621 acgcaacaat agcaaacctt gtgggacgcg aaaccgttgc ttgcgcaatc gaccttggat
     4681 tggtcgatcc tcaatgtatt ttgaagatca aaggagtccc tcacgcccag attgttcaga
     4741 tgatttgagt ctcacaatga ttcttttttt atgagtgtgt tgattttttc cctcatattc
     4801 tcgtagtgcg tccccatcca ataatactta gaacaggagg gacaaaacca gaactcattt
     4861 cttgtctgaa ataccttctc aggtatctga tctttaatag cttctttcgc aatagagtga
     4921 agcggggtat tgcatagtgt acaacgagta aggatttttt cctcatcgag ggtagtctcc
     4981 ttgagcacca gctgtaattg ttcggtgaga tctgttgtat gtatctcaag aacaggcacg
     5041 agcgcttttt tcgctcgtat cagtaattcg ttatcccggg agataagcag ccgtttctca
     5101 cgctttgcga tctgaaggac aatatcgtca ttcatcgtcg catctgggta gacagtatca
     5161 aatccaaaaa ttcgtagcca tttcgcaagc gaaccaagca tgtgatcgca gaacagtttc
     5221 atccgagggg ataatggaga ggagggattt tgtcttttct gacactgcat ttattccttg
     5281 tattagaggt attttctgac ttcaagcatg tcgtagttgg gatttccagc gttatcgtat
     5341 gcgatcacaa tgatggtgtg tttgtgcata aggagcgatg gtgtggacca tacccagcta
     5401 tagggtgctt gggtatcgac aaatgctgga gtaacagaat catctatgag gaactcgact
     5461 tgcttaatat ctgattgtgc atctgctgct tccacctgga tagtgatcgg accatagatg
     5521 attgtacttt ttctgaacaa cccggagaac aggcgaaggc cccagagata caacccgtta
     5581 cttggggttt gtatcttgac gatgggcggg gtcgtatccc caccagtgta gggtgttatc
     5641 agcggatatt gatctgtgtt gatcccatct gggatcgcat aggagatatc accgatacca
     5701 tcaccatcac tatcagaacc agtgtaatca ctccaatagt tccctcctga tggatacccg
     5761 tcatcccaga tgttagctgc attatcatag gcttgatgac ggtttgattt gaaattatta
     5821 tgataaatgt aattatttga ggattcagaa atccctatcc ctgcgtagaa gctgtcaatg
     5881 atggtattct caagtatgta gttacctgta caacctggct tcagggtgac tccattccac
     5941 ccttggccag agattttatt atctattatc tgagtaccct tggtagattg tttcaggatg
     6001 attccttgga agttatcgta gatagtattt tgctcaatgg aacaattatt cccatttacg
     6061 gcgatcccag cgttttgttc cgctgttcca ctgtgctgaa tcatgaatcc tataagattc
     6121 acattatcag cgctgatagt tatgacatcg ccggagtatc tgccatcgat aatactagta
     6181 tcaccatgga ctccatggag tatcaaggat ttggtaatgc agagatgctc ccaatagact
     6241 ccgacactaa taacaatcaa atgaccattc tgggtgttat catcatcgat tgccgcttga
     6301 atcgataaga aaatctcatc ggtatctgcg ttgcggatgg aaccataggg gtggatgagc
     6361 ggatattgat cgacagagct attccctgtg gggtagggga tatctccgat cccatcctca
     6421 ttctcgtctg aactattata atcatcccaa taatttcctc cagtgggata tcctccatcc
     6481 caggcgctgc tcgcgatgac atcaaacgct tgataggtat tctcaacaaa gttattgtga
     6541 tagatagtat tgtcgtatcc ttcaatcaca tagattccat agtagttgtt tttttgtatg
     6601 ttattattga taatagtgtt gtaaggtgta tgaatcatga tgcctgcgtt gttcggattg
     6661 ctcccactgc gagtaatagt aaatccagtg atcgtgacgc cttctgcgat gattttaaca
     6721 acggtcccga aatcattacc tgtaatcact gtgttttctt tatcttcgcc gatgatctcg
     6781 agtgacttgg agactacaat attttcttta tagataccat tgaagacaga tacgatatct
     6841 cctccgatcg catcatctat accatcttgg atataccggt agggatgatc tatagtcccg
     6901 tcccatggtc ctgcggtgtt attatcgtca acgtagatgg tcgatcctcg tcccacggtt
     6961 tgctcgctat tcttttgtag ttcggtaaaa acgcttgcag atcccggaat gataaacaga
     7021 cctgcgatga caaaagtaaa aagagcttga aagatagatt ttttatgatg tgttatgttc
     7081 atatttattc ctcctgcgcc taaacaatac ttagtatagt ttttatctct tataatgtta
     7141 tattaataga atatttaaat atttgccgat aaatgataag gagtgcatcg tcaaatgttc
     7201 aaaaagcgtc cattcttgta gatcaattca tcatcggcgt agatttcccc gttttttcgc
     7261 aggtcgcaga ccatatccca atgcaaactg gatttatttt tactcccggt ctcagagtat
     7321 ccactgccga ccgcaagatg gatagtccca ccgatttttt catcaaacag ggtgtttttc
     7381 gtatagttct tcactccgta gttcgtcccg aacgcgaact cgcctaatcg ttttgcacca
     7441 gggtccatgg caagcatggt atgaaggaac tgttctcctt ttgatgcggt tgccttcact
     7501 acgacacctt tcttgaacca gagctggatg tcattgactt cccggccgtt atacgaacca
     7561 gggaatgaat agctgatgtg tccgtccgct gaggtttcta ctgggccggt gaacacctcg
     7621 ccgtctggga aattctctgt gcccgaacag ttgatccatt tcctgcctgc gacagatatc
     7681 cgaagatcgg tatccgtacc aataacatgg agggtctttt ttttattaag gaatgttcgt
     7741 atcttttcct ggtcgctctg gacggttctc caatatttga taggatcttt tgcttggaca
     7801 tgggcagcat tgaaaacaaa attctcataa tcctcaagag acatctcagc gtcttgggcg
     7861 gacgcctggg ttgggtactg ggtgatgcac cagcggagtt ctttttttgc agcccggttc
     7921 atgaatattt catgaatctg atgatgcgca acgttgctga ttgcctgttt tttcgggtcg
     7981 atgttggtca tgttgcgcgt gttctcaggg ctgatgatgg aaaggctcgc atcgatctgt
     8041 tcgatttcgt atttactgag aggcgagacg tatttgagtt gttggtctga actgttttga
     8101 taatagatct ctgcaagacc atcgatgccg atatgggtga aaggatgtgc accaatctgg
     8161 actgcttggt aatagacttc tttgatcaaa ggtgcagcga gatgactgcc tgagatgaca
     8221 aagagatcat ttttcttcag gtttaatgag taacgggtta aaatcgtggc gagtttctct
     8281 aagcgtttgt caatcatatt tctatcccag tctgagaaca ggtgaaatca taaaaggatt
     8341 tccagtcgcc cttagatttt ctcccagtcc tttaacacgg attcccagtg atatttttta
     8401 attgcttcga tttcatcgag ttttcgtatg atacgttcgt agcctttgat gagcgttcgt
     8461 gtcgacacct tgacgatgaa atcgtgtttt cctgaggtaa tcgtcacatg ggagactcct
     8521 ctgatgtccc agagtttctg taacgtctct ttcaatggtc tgccggtttt cacatcgatg
     8581 aagatatacg ctttgacttg ggtgagtgca aaggagataa tacccatgac gatcaagacc
     8641 attccaatga ctttgataat caacatgaca ggatcggtag aaaattcagt aatagttcct
     8701 aactgcaata ggataattga gaaggggatc gcaaagatga tggtcgaggc acgtatcgcg
     8761 ttattcacac ttgcttttcc cataccaagg gaacgtatat ataaaacgta ggagaagaac
     8821 gtcgtcccca tggtcaaaca gagtaagccg aaatagaacg gatcgattgc agcagtaatg
     8881 ccagagagga gatgattccc gctagtaatg atatcataga taaagaggag gagggcggtg
     8941 aaaagacagg aaaaaatcac attccagaat cggatattaa tcgaatcatt cggtcgattg
     9001 ttgattctca tgatctttaa ttttctttga taaatcgaga agatcgccca ggtggggttc
     9061 accacgagga agacgatgag gatcgggaat agctgaaact ccccggtgag gcttatagat
     9121 gcaaggattg ctccgaaggt gacgatcacg gaggattgta tctccacgag tgtcggaacg
     9181 tctttttctg tgatggattc catgaggagg agatacagga tgacgatctg ggagaacgat
     9241 aggactatac tagggtcttg gactgcggag ataatcgcgc agtacccgac ggtattgatt
     9301 gcgttgacca gaccagcgat ggaatggtat ttcatctcgc ttttctggac catacggatt
     9361 tgtttgaagg agggatctag tatttttgcc ccaatgcttt gtcctttgat ttttactgat
     9421 agagcgagtg ttatgaggag ggtgatgacc acaccaatga aaaatagtga aaaagcaaac
     9481 gaccacggat cggttttaaa cgcgggttga ccgccaacaa aactatcgac gccggtgact
     9541 gccgcagcaa taaatgaaga cgtgatcgcc aggacataaa agagatattt tttcctaatg
     9601 atcatgtttt ggtctcatta ttgaaggctg atttcttttt caatgatttg ggtggttgtc
     9661 ttggtgactc cttcgatcat ttggatttta tcggtgacgt ctaagagttc ttcgaggttt
     9721 tttacctggg ctctgacgac aatatcgtat tcccctgcaa taatggagat ttttgcgaca
     9781 taatccaact gcctcatttc ctttattgct ttgtcggttt tcccgggttg catcgtgatg
     9841 aggatgtacg cgcttatcat agttcttcac ccatctttca cgcggaatat tgacgcttgg
     9901 tataaagttt cctgtatatg agtatgtagt gtttccaggt taatatacgc aattttccat
     9961 aaatgttgta gtaacatctt tcaagactgt tgagaca
//
LOCUS       DATMXJ010000085         6646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_6776, whole genome shotgun sequence.
ACCESSION   DATMXJ010000085 DATMXJ010000000
VERSION     DATMXJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6776"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="VN377_02470"
     CDS             <1..2227
                     /locus_tag="VN377_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27282.1"
                     /translation="SQIDNPQTTPTREGLNWWNTQWQYRKLITIDHTLVCSDLVNFPV
                     LISEVTDLDLAAAAQPNGQDLVFIRYTDNSTILNHEIESYNHLNGQLTAWVKIPTLSA
                     TSDTKLWMYYGNPTCPSQEHITGTWDNNYVMVQHLNETGTTMDDSTSYHNNGVSTGTN
                     YTDAGKIDGSQLYNGNDKIVVNNLTNSPTALTIETWVYRDSTTFIYIACKGTYSNTND
                     WILYLRNNQTADEGIDFGIRNHTAYIRKGDTPVNSWFHLTVTYDNGNVALFLNGINIA
                     NATSWPAIPNMYPHLGLGNDYMGTEGGYYPMTQVMLDEFRISNTARSTGWITTCYHNQ
                     NNPESFYSMGAEEQYELTLTTTSDPPQGGTITASPDPPYYYNDIVTLTATPNPGYLFD
                     HWSGDLSGSTNPTTITMNANKAVTATFILEDENEPPVANDDYATVQENSTNNQINVLK
                     NDSDPDGDNLSITSVKQPLHGNATTDGLFAYYTPNASYFGSDSFTYTISDGAGGTASA
                     TVYITVIALPTNNPPNKPSQPIPDNGESNVNITTDLWWLGGDPDTGDTVTYDVYFGMT
                     TTPPKVESNQSNNTFTPILTYETTYYWRIVSWDNHNASTQGDLWSFTTQGEQHESINV
                     TITRPLENSFYLRNFRLLRNTRSTTVYGPITITASVTVTNTTVARVEFYVDGKLKRTD
                     DTAPYTYRWAPLRSFKHTIKVIAYDADQESAIDQITVFKWRLHPVLLIGGAYLLSKIM
                     K"
     gene            2388..4373
                     /locus_tag="VN377_02475"
     CDS             2388..4373
                     /locus_tag="VN377_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR27283.1"
                     /translation="MVRYRTVRWTKKLKKITASFLIFILLSLLLPLVNPGETTSPSTW
                     YISPTGNDRNPGDITNPFKTLQKAINASSNGDTIYLRAGHYNTIFSTTKGILIDRSGT
                     PDSWFTISGYPGERAVINGRGYTLAPNSALLRIGTAAKGQSYVRITNIIIENSSRDGI
                     RVAQQGIQTSHHIRVDNVTINNCAYRGLMFWSTNPNKAWCHNLTVENCTINNTQSSRS
                     MGEGITFSGCKDITFHHNTVTHCPKIMLDLANNTKNCNIYKNTIVTTQGSGIKLDGSQ
                     SNKVNAWDENISIYNNHFSGSYTAIKIGVEKKGGCKNISFYNNIINTVSSYPGVKLEG
                     ATGYSQLLDNIVIKHNTIQVSGTGSPLQVEQPPAKLRNIYVANNIFQGDGSASWQVRS
                     SVSNFTDSCFHFYNNIYNHTTKPANTAWVDGRHKFETTAILSSPQFVNRKTGDFHLNS
                     TSPAIDAADSTYTVPFDYDGRTRPQNTIPDIGAYEFSAMNPPINPPTITNVQTTPEQQ
                     QIGGYINISCEITENDAPVDNVWVNITYPDATTHNYSMNKGFYLNQTYDQIGDYQFLI
                     WVHDTNDNTNISTGYVFHIMPVLHSTFIVGLISTNQYAGGPTLYLNAKLLLYIEINPL
                     TVTLLSSNEKILISKEYIGYVGPKFIIGVFNGVVLSE"
     gene            complement(4466..5368)
                     /locus_tag="VN377_02480"
     CDS             complement(4466..5368)
                     /locus_tag="VN377_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR27284.1"
                     /translation="MHKPTRKRTLAIIPCRNEEATIGSVILKTKRFVNQVLVIDDGSD
                     DETKKIAKEAGAMVISHKKNRGKGAAIRTGFQYAKENDFDYVVTMDGDGQHNPLEIPV
                     LLKNVVNNGHDISIGYRVGKGTEMPMWRKVGKRVLDYTTSMGTGGFVTDSQCGFRAFN
                     KKAVESIAPTLKGNAFSVESEQLINAHELGLRVINTNVTCKYKDLDSSTKNPASHGLS
                     VLGYILWVIAEKRPLLFISVPGFVLAVIGILLGIYTIQFLNQNSIFLATYAFMANILL
                     IIGAFAILIGLVLHVIPRFIKKFG"
     gene            5636..>6646
                     /locus_tag="VN377_02485"
     CDS             5636..>6646
                     /locus_tag="VN377_02485"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HWR27285.1"
                     /translation="MKTNLITLGLLLMLLCAIPIVVATSPTPTIRLPTVLVTMNAVYG
                     DNSYFDMTLSDIPLGYDVINGTYHGWCVQETIKMTQHVNHTVLLYSCYDPSLPLEYHN
                     SHWDKINYLLNHKQGNANSIQQAIWYYTNNKDCSADPDASAMISDAERHGAGFTPNIG
                     ELLAVPIQGVPAIQLTFLETTIPAPSTLEGFVWDDTNANGLQDKGEPGMNTITIYLYQ
                     QNNFLINTTTTNSKGTYHFTGLPKGAYYIQVTLRPGYHFSPQNIGTNDTIDSDVDITG
                     LTPLFLITINQSPQRWDAGMYTTAPQKPHNPQNHRPTADGTAGEPYNGFVHELITFDG
                     SNS"
BASE COUNT         2118 a         1806 c         1193 g         1529 t
ORIGIN      
        1 atcacaaata gataatccac aaaccacccc aacaagggag ggcctcaact ggtggaacac
       61 ccaatggcaa taccgaaaac tcatcaccat cgatcatacc ttagtctgct cggatcttgt
      121 caatttcccg gtgttgatct ctgaggttac tgatctagac ttagcagcag ctgcacaacc
      181 aaacggacaa gacctcgtct tcatccgcta cactgacaac tccacgatcc tgaaccatga
      241 aatcgaatcc tacaaccatc tgaacggcca attgaccgcc tgggtaaaaa tcccaacgct
      301 cagcgcgaca agcgacacca aactctggat gtactacggg aaccctacct gcccctcaca
      361 agaacacatc acaggcacct gggataacaa ctatgtcatg gtgcaacatc tcaatgaaac
      421 tggaaccacc atggatgatt caacatccta tcataacaat ggagtatcaa caggaacaaa
      481 ttacacggat gcaggcaaaa ttgatggcag tcaactgtac aatggcaatg ataaaatcgt
      541 cgtaaataat ttgacaaatt ctcctactgc tttgaccatt gagacctggg tataccgcga
      601 ttcaaccact tttatttata tcgcctgcaa aggaacctat tccaacacca acgattggat
      661 actatatctg agaaataacc aaacagctga cgaaggaata gatttcggta tccgaaatca
      721 caccgcctac atcagaaaag gagacacacc agtgaacagt tggttccatc tcaccgtaac
      781 ctacgacaat gggaacgttg ccttattcct caatggcatc aatattgcga acgcaacaag
      841 ttggccagca atccctaata tgtatccaca cctcggcctg ggaaatgatt acatgggaac
      901 cgaaggcgga tactatccca tgactcaggt gatgcttgat gaattccgca tttctaacac
      961 cgctcggagt accggctgga ttaccacctg ctatcacaac cagaacaacc ctgaatcttt
     1021 ttattcaatg ggggcagaag agcaatatga actcacgctt acgacaacca gcgatccacc
     1081 ccaaggaggg acaatcaccg catcaccaga tccaccatac tactacaacg atattgtcac
     1141 actcaccgcg acccccaacc caggatacct gttcgatcac tggagcgggg atctcagcgg
     1201 atctaccaat ccaacaacaa ttaccatgaa tgcaaataaa gcagtcaccg ccaccttcat
     1261 ccttgaagac gaaaacgagc cacctgttgc aaacgacgac tacgccacag tccaggaaaa
     1321 ctccaccaac aaccaaatca atgtcctgaa gaacgactcc gacccagatg gggacaacct
     1381 ctccatcacc tccgtcaaac aaccgctcca tggcaacgcg acaacggatg gactcttcgc
     1441 ctactacaca cccaacgcat cgtacttcgg atcagactcc ttcacctaca ccatcagcga
     1501 cggagctgga ggcaccgcat cagcaaccgt atacatcact gtgatcgcac ttccgacaaa
     1561 caacccaccg aataagccga gccagccaat ccctgacaat ggagaatcca acgtcaacat
     1621 caccactgat ctgtggtggc ttggaggaga ccctgatacg ggggatactg ttacctacga
     1681 tgtctatttc gggatgacca ccacaccacc gaaagtcgaa tcaaaccaat ccaacaacac
     1741 ctttactccg attctcacct atgaaaccac ctattactgg cggatcgtct cctgggacaa
     1801 ccacaacgca tcaacccagg gagacctctg gagtttcacc acacaaggag aacagcacga
     1861 aagcatcaat gtcaccatca cacgaccgct cgaaaactcc ttctacctac gcaacttccg
     1921 actcctcaga aacacgcgaa gcaccaccgt ctacggtccg atcaccatca ccgcctcagt
     1981 caccgtcacc aacaccactg tcgcaagagt ggaattctac gtcgacggaa aactcaagag
     2041 aacggatgac accgccccgt acacctaccg ctgggcacca ctccgcagct tcaaacacac
     2101 catcaaggtc atcgcctacg acgccgacca agaatctgcc atcgatcaaa tcacggtctt
     2161 caaatggaga ctccacccgg tccttctcat cggcggagca tacctcctat caaaaataat
     2221 gaaataatcc accacacacc tacagagaac ctatcgtggc atcgtcgtac cagagacgat
     2281 actaccatac tctggactct ccaaaacaac ctctttttct tttttagtat ttcactatat
     2341 ggctatagac aattttatat aataagacgt attgcataat aatggatatg gtcagatata
     2401 gaaccgttag atggacaaaa aaattaaaaa aaatcaccgc atctttcctc attttcatat
     2461 tactcagcct cctcctcccc ctcgtcaacc ctggtgaaac gacctcaccg tccacgtggt
     2521 acatatcacc aacaggaaat gatagaaacc caggagacat caccaaccct tttaaaacac
     2581 tacaaaaagc aatcaacgca tcaagcaacg gggacacaat ttatctacga gcgggacact
     2641 acaacacgat cttttcaacc acaaaaggga tactcattga cagaagcgga acaccagatt
     2701 cttggttcac tatctcaggc tatcccggag aacgagccgt catcaatgga cgtggatata
     2761 ccctggcgcc caactctgct cttttacgca tcggaacagc agccaaagga caaagctatg
     2821 tcagaatcac aaatattatt attgaaaatt cctcaagaga tgggatacgg gtagcacaac
     2881 aagggattca gacatcacat cacatacggg ttgacaacgt tacgataaac aactgtgcgt
     2941 atcgaggatt aatgttttgg tctacaaacc caaataaggc ctggtgtcat aatctcacgg
     3001 ttgaaaactg tacaataaat aatacccaat ccagccgctc catgggtgag ggaatcacat
     3061 tttcaggatg caaagatata acgtttcatc ataatacagt gacgcactgt cctaaaataa
     3121 tgcttgatct agcgaacaac acgaaaaatt gtaatatcta taaaaatacg atcgtcacaa
     3181 cccagggatc tggtattaaa cttgatggaa gccagtccaa caaagtcaac gcatgggatg
     3241 aaaatatcag catttacaac aatcactttt caggcagcta cactgccata aaaatcgggg
     3301 tggaaaaaaa aggcggatgc aaaaatattt ccttctataa caacatcata aacacggtaa
     3361 gttcctaccc tggagtgaaa cttgaaggag caacaggata ttcacaactc ttggacaata
     3421 ttgtcatcaa acacaatacc atccaggtgt ctggcacagg aagccctctt caagtcgagc
     3481 aacctccagc aaaactgaga aatatttatg tggcaaacaa tatttttcaa ggggatggtt
     3541 ctgcatcatg gcaagtgcgt tcctccgtct ctaacttcac tgattcctgc ttccatttct
     3601 acaataatat ttacaatcat acaacaaaac cagcaaatac agcatgggtg gatggaagac
     3661 ataaattcga gacgacagca atcctatcat caccacaatt tgtcaataga aaaacaggag
     3721 acttccatct caatagtacg tctccagcaa ttgacgctgc ggattctacc tataccgtcc
     3781 cgtttgatta tgatggacga acccgtccac agaatacgat tcctgacatc ggtgcatatg
     3841 aattttctgc catgaatcca cccatcaacc ccccaactat cacaaatgta caaaccaccc
     3901 ccgagcagca acagatagga ggatacatca acattagctg cgaaatcact gaaaacgatg
     3961 ctcctgttga taacgtctgg gtgaacatca cctacccaga tgccaccaca cataactatt
     4021 ccatgaacaa aggattctac ctcaaccaga cctatgatca gataggagat tatcagtttc
     4081 tcatctgggt gcatgataca aacgacaaca caaatatctc aaccgggtac gtgttccaca
     4141 tcatgcccgt gcttcatagt acatttattg ttggattaat tagcactaat caatatgcag
     4201 gtggaccgac gctgtatctc aatgcgaagc tattactcta cattgaaata aacccgctca
     4261 ccgtcacact gctttcttca aatgaaaaga tactcatctc aaaggaatac atcggttatg
     4321 taggaccaaa atttatcatc ggagtattca atggagttgt cctttcagaa taaagtctaa
     4381 attgtgatga gacaaaaaat attagacaat actcctttta atgatgtaat tcctagaaat
     4441 gaatacatca aattcaatac aacgcttatc caaatttttt tataaaacga ggtatgacat
     4501 gtaaaaccaa accaatcaag attgcaaaag cgccaataat taacagaata ttcgccatga
     4561 aagcatacgt agcaagaaaa atactgtttt gatttaaaaa ttgtatggtg tagatcccta
     4621 agagaattcc aatgaccgct aaaacaaaac ccggaacact gataaataac aagggacgtt
     4681 tttctgcgat aacccagagg atatacccaa gcaccgaaag accatgtgac gcaggatttt
     4741 ttgttgacga atctaaatcc ttatatttac aggtcacatt cgtgttgatg actcttaatc
     4801 ctaattcatg ggcattgatc aattgttcac tttcaacgct aaatgcattc cctttgagtg
     4861 ttggtgcaat ggattcaacc gcttttttat taaacgcccg aaaaccacat tgactatcag
     4921 taacaaatcc tccagtaccc atgctcgtgg tataatccaa gacccgcttc ccgacctttc
     4981 gccacatcgg catttcagtg cctttcccaa cccgataccc aatagagatg tcatggccgt
     5041 tattgaccac gttttttaac aacaccggga tctcaagagg attatgttga ccgtcaccat
     5101 ccatggtgac cacgtaatcg aaatcgttct ccttcgcata ttgaaaacca gtacgaatcg
     5161 ctgcgccctt ccccctgttt ttcttatggg agataaccat ggctcccgct tctttcgcga
     5221 tcttctttgt ctcatcatcg ctcccatcat caatcaccaa cacctggttg acgaaccgtt
     5281 tcgtcttcag gatgacgctg ccgatggtcg cttcttcgtt gcggcagggg ataattgcaa
     5341 gagtgcgttt tcgagtgggt ttgtgcattt tttttcctcc ataagtcatg cgggatgaac
     5401 ggataaagga cttgcctcag ttctatcgag tgataatcag cctttcgaag tatatattca
     5461 tttgagggtt atttaaatat tttgtgacag ttgttatcgc ttttcgacga ttttttcaga
     5521 agaaatgatg aggttgtacg aaagacaaga aatgttgaga agcgaaacgt ttaaatcatc
     5581 acaaaacaac tttttttaca atacaagtaa aatgttagtg aggaaagaaa cccctatgaa
     5641 aaccaaccta atcacccttg ggctccttct catgctcctc tgcgccatac ccattgtagt
     5701 ggcgacatca cctaccccga ccatccgatt acctactgtc ttggtcacta tgaacgcagt
     5761 ctacggagac aacagctatt ttgacatgac actctccgac attccgttag gttatgacgt
     5821 tatcaatggg acctatcacg gttggtgtgt gcaggaaacg attaaaatga cccagcacgt
     5881 caaccatacc gtactactct actcctgcta tgatccctca ctgccccttg aatatcacaa
     5941 cagccactgg gataagatta attatctcct caaccataaa caaggaaacg ccaacagcat
     6001 ccaacaagca atctggtatt acaccaataa caaagactgt tccgcagacc ctgacgcctc
     6061 cgcaatgatc tccgatgcgg aacgacacgg ggctgggttc acccctaaca tcggtgagct
     6121 actcgcagtc cccatccaag gagtaccagc aatccaactg acatttctag aaacaaccat
     6181 cccggctcca agtaccctgg agggcttcgt ctgggatgac acgaacgcca acggtctcca
     6241 agacaaggga gaaccaggga tgaacaccat cacgatatat ctctaccaac aaaacaactt
     6301 tctgataaac accacgacca caaacagcaa agggacctat catttcacag gcctccctaa
     6361 aggtgcctac tacatccagg tcactctccg acccggctat cacttcagcc cacaaaacat
     6421 tggaaccaac gacacaatcg acagtgatgt cgacatcaca ggactcaccc ctctcttcct
     6481 catcaccatc aaccaaagcc cgcaacgatg ggacgcagga atgtatacca ctgcacccca
     6541 aaaacctcac aacccgcaaa accaccggcc caccgcagac ggtaccgctg gtgaaccgta
     6601 caacggtttt gtccatgaac tgatcacctt tgatggatca aatagc
//
LOCUS       DATMXJ010000086         6309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_1716, whole genome shotgun sequence.
ACCESSION   DATMXJ010000086 DATMXJ010000000
VERSION     DATMXJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN377_02490"
     CDS             <1..306
                     /locus_tag="VN377_02490"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWR27286.1"
                     /translation="EQSYRFYVERLFRFFEKIGATTFLITETQQIPEVFSPTGVEEFL
                     ADGVVVIYNIRRQNTRENAIEVLKMRGAKHQKKIVSMNITEKGIVVNPNKEVYSEIE"
     gene            complement(363..2552)
                     /locus_tag="VN377_02495"
     CDS             complement(363..2552)
                     /locus_tag="VN377_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HWR27287.1"
                     /translation="MKTPRTTKAVSQIISEILLLAIAIISVSIIYTQVLGAPGPQDTA
                     NVTIIGKIEDGHPVFDLQRGESLERDTKIYINIAGGYNKSIYSLDQLFMQNFIGNQVW
                     NIGEQIVLPSENLTGYGGPQVEGTIVDAKTNAIVFWGILQEGIITGHKGGTWHFDEDH
                     WNLNISNEVKDSSGNKNHGIAKNNAIIINGTTQPSNVAHNNSGYFNVLKDAYVRVPSS
                     WSLNISDAITVEAWMKPQLLPPILDIVDLEEKFGYTPYIIYVGGDCYAIVSEDQGKRG
                     TLQTVFIDPEGGITNKSVEYFGNSQGSILLRPMITQMTDSMYLVSFNSKIGGNLYIHL
                     RTYNISTDGSIEYTGYELVFQDYTSSANQPNRASLQKITDKLYAIAYWVPNYGGILKT
                     LTISPTGEFDASSVKMIRYDPVAGYEPSLINVTQNVTALAYRDASNHGILKTFNISST
                     GDISYTGKTTPFDSSSGYEPSLIQVSGKVYAVAYRNTDNDGLVKTLNLSSNGSIAWTG
                     NMLTFEDNVPCFDPSIIHAEDDFYVVAYSTGNSGSTTGYIMSLKLGKTGSILSQTESR
                     REFKIDGRDRCYNPIILHIYEHLFAISFTGPTDHPGELITINIGTDTYPPHRGITKQD
                     SYGIFANATTVVGSINNIMVSAPISSGWHHFAVTYDGVSIRLYVNGSLKGSTLYPHHQ
                     INMTPSDLLFGRNYHGYIDEIAIYDQALTQIQIQNHVNNRGTFEPFT"
     gene            complement(2664..3521)
                     /locus_tag="VN377_02500"
     CDS             complement(2664..3521)
                     /locus_tag="VN377_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27288.1"
                     /translation="MKKFKKSDNAVVGVVTAILLIGLIVAVISLVQTIYIPKIMEQRE
                     AEHMDKVAEQFTYLTSVIDGQAADQSKNMPVATSITLGSKELPYMLSIKAFGTIEILD
                     NARHINITLADGTVKTFPVGVITYSSLNGYFLDQSYTYEAGAMIVSQSEGNLMMIHPG
                     FFIDYDSVQRVLNISFDVVNITSVGQKTIASGFGTYPIQTEFSKVSSNVTFTDIREIT
                     LKTPLSNAWDVFMNSLLKTSVNENDYDLTDTGYSLILNNFSSLTVNVFFKIIDIRAQI
                     GPGWVEQQG"
     gene            complement(3660..5186)
                     /locus_tag="VN377_02505"
     CDS             complement(3660..5186)
                     /locus_tag="VN377_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HWR27289.1"
                     /translation="MKKKRRSNHAVSEVLATVLLLGITIALFGFLNYIVFSFSFESPA
                     PSVSLIGSFSADGTHNNITIEHNGGESLDGNTEIIITVGATTNKSTVRDIIDGTTDWK
                     LLPLKDDKNPDKWDFGETIQFNSRYDFTDTIIQASVMDPSTNTLILSVVLQHGPATSV
                     ITNKPPTITTPLPTNGSTGNLLGLTWKIQIDDPENDPFTWTIQCSNGQTNNGAGPNGQ
                     KTLILTGLSYSTTYKILVNATDPTGSNLFTRKWYTFTTTGNLPPAFGTPTPSNGSINQ
                     PLNLPWNIPINDLEGNPFTWTTQCSNGQTNSAAGATNGTKTLSLSGLSHSTTYRIWVN
                     ATDPTGSGLWTRRWYTLTTKTSTVINLKPNLVYDNTSGSDYTLTPSNRLNLNLSDNLK
                     YRSQAQWTNNFGKRIEFNFPDIPTGATVINVTLKFEWNITGTAGTTNARLMIYNGSNW
                     QVIALIPSTTDVIVTKYLLGNINNAAKVNSLKIWFQVSTNKNYYTWNDWVQVDVTYTQ
                     "
     gene            complement(5201..>6309)
                     /locus_tag="VN377_02510"
     CDS             complement(5201..>6309)
                     /locus_tag="VN377_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR27290.1"
                     /translation="DLDLLGVIAEIFVTCAIAFPIFLVIILTVFGFFGGSSVSSIVIL
                     NIFSLVMMPLIYAGFYFLIRSTSLEDLNRIYSKENLTFRQYLKENETPIMILLLSSLI
                     VVISYIGLRVMDYLGYISFTFYPQLDFIFLAILLLIGPIGTYNYFEAQKKKQIQYRLP
                     NFLIEVSDSLSTGATIFDAIKVASKSHYGRLSTEIKKMRSQLSWNIAVKEILIDFADR
                     MKSAIIHRIVISLNKGLVMGGNTPKIFRAAAIEVSQVNRLENQRKATMSIYTVVILVC
                     FFVFLAIIIIMNLTIFKSFFDLQGKQVGRMAGVVFTSVDRMELKYSLYSFVFVQSIGA
                     GILSGFMMDGKISSGIRYSCILGIISFIIFKLTF"
BASE COUNT         1549 a         1200 c         1580 g         1980 t
ORIGIN      
        1 gagcaatcct atcggtttta tgtggaacgt ttattccggt tttttgaaaa gattggtgcg
       61 acgacgttcc ttatcacaga gacgcagcag atccccgagg tgttttcacc gaccggtgtc
      121 gaggagttct tagcagatgg ggtcgtcgtg atctacaata tccgacggca gaacacccgg
      181 gagaacgcca tcgaggtcct gaagatgcgt ggagcgaaac atcagaagaa gatcgtctcc
      241 atgaacatca cggaaaaagg gatcgttgtc aatccgaaca aagaagtcta ttcagagatt
      301 gaatagataa tcaagagtcg acacgggttc ttgagtatcg tggtttgcta ttcttggaat
      361 aatcaggtaa atggctcaaa cgttcctcga ttgttcacat gattttgtat ttgtatttga
      421 gtaagagcct ggtcataaat cgctatttca tcgatgtatc cgtggtagtt ccgcccgaag
      481 agtaaatctg atggggtcat attgatctga tgatgtggat acaatgttga tcctttcaat
      541 gagccattga catacagtcg tattgacacg ccatcatatg tcaccgcaaa atgatgccag
      601 cctgaggaga tgggggcgga gaccatgatg ttgtttatac tccctacgac cgtggttgca
      661 tttgcgaata tcccgtagga atcctgtttg gtgatccctc ggtggggtgg ataggtgtcc
      721 gttccaatat ttatcgtaat tagttcacct ggatgatctg tgggtccagt gaagcttatt
      781 gcgaacagat gctcatagat atggagaata attgggttat aacatcggtc tctaccgtct
      841 attttgaatt ctcttcgtga ttctgtttgg cttaaaatcg agccggtttt ccctagtttc
      901 agtgacataa tatagccagt ggtgctcccg ctattccctg tagagtaagc gacaacataa
      961 aaatcatctt cggcgtggat gatactgggg tcaaagcaag gaacattgtc ttcgaatgtc
     1021 agcatgttac cggtccatgc aatggaaccg ttcgatgaga ggttaagggt tttcaccaat
     1081 ccatcattgt cagtatttcg gtatgcgact gcatatacct ttcctgaaac ttgtatcaga
     1141 ctgggttcgt atccggatga tgagtcaaac ggagtcgtct ttcctgtata actaatatca
     1201 ccggtggatg agatattgaa tgtttttaga ataccatgat ttgaggcatc acgatatgct
     1261 agagctgtga cgttctgagt aacattgatg agagatggtt catacccggc gaccgggtcg
     1321 tatcgaatca tttttacgct agatgcatca aattctccag tcggtgagat ggtcagtgtt
     1381 tttaggatac ctccatagtt tggcacccag tatgcgatgg catagagttt gtctgtgatc
     1441 ttttgtagac ttgctcggtt tggttgatta gcagaggagg tataatcctg aaatactagt
     1501 tcgtatcccg tatattcaat cgaaccatcg gtcgaaatat tatatgttct caggtgaatg
     1561 tacaggtttc caccaatttt ggaattgaat gaaacgaggt acattgaatc tgtcatctga
     1621 gtaatcattg gtcggagtaa tatgctccct tgggaattcc cgaaatattc aacactttta
     1681 ttggtgatcc ccccctcagg gtcgatgaaa acggtttgaa gtgtgccgcg ttttccttga
     1741 tcttcgctta cgatagcata gcaatcgcca ccgacgtaga tgatgtaggg tgtgtacccg
     1801 aatttttctt caagatcgac gatgtcgagt atcggtggaa ggagttgtgg tttcatccat
     1861 gcttcaacgg ttatcgcgtc gctgatgttc aaagaccagg atgaaggaac acggacgtat
     1921 gcatccttaa ggacattgaa atatccggag ttattatgag ccacattgga aggttgtgtg
     1981 gttccgttga taatgattgc attgtttttt gcgatgccat ggttcttgtt tccagaacta
     2041 tctttgactt cgttagagat attaagattc cagtgatcct catcgaaatg ccatgtcccg
     2101 cctttatgtc cagtgatgat gccttcttgg aggatccccc agaagacaat ggcattggtt
     2161 ttcgcatcaa caatggtgcc ctctacttgt ggtcctccat atcccgtgag attctcggat
     2221 ggaaggacga tctgttctcc tatgttccac acctggtttc caatgaaatt ttgcatgaag
     2281 agttgatcaa gggagtagat gcttttgttg tatccccctg cgatgttaat gtaaatcttt
     2341 gtatcgcgtt ctaaggattc tccccgttgc aggtcaaaga caggatgtcc atcttctatt
     2401 ttaccgatga tggtgacatt ggctgtatct tgcggtcctg gcgcccctag tacctgtgta
     2461 tagatgattg aaacagaaat gattgctatt gcgagtagaa gtatttcgct gattatttgt
     2521 gatactgctt tcgtggttcg tggagttttc atttatgcat ccccctcgta tattatattt
     2581 atattaattt ttatatgaaa ataagtatat ttaccgctat ataaacgttg ttgtacaaaa
     2641 acagtctata tttttccgtt gttttatccc tgttgttcga cccagccagg gccgatctgg
     2701 gctcgaatat cgatgatctt gaaaaagacg ttcacggtga gagaagagaa attatttagt
     2761 atcaaagaat atcctgtatc ggtcaggtcg tagtcattct cgtttacact ggtttttaac
     2821 aatgaattca taaaaacatc ccaggcattt gacagtggtg tcttcagagt gatctcacgg
     2881 atgtctgtga acgtgacatt gcttgatacc ttagagaact ctgtttgaat cggatatgtt
     2941 ccaaaacctg aggcgatcgt cttttggcca acactggtga tgttcacgac atcaaatgaa
     3001 atatttaaga ctctttgaac tgaatcgtaa tcgatgaaaa agccagggtg aatcatcatc
     3061 agatttccct cagattgact gacgatcatc gctccagcct cataggtgta tgattggtca
     3121 aggaaatacc cgttgagaga cgagtaggta atcaccccga cagggaatgt ctttactgta
     3181 ccatctgcca gtgttatatt tatgtgacga gcgttatcca gtatctctat tgtaccaaat
     3241 gctttgatcg ataacatgta gggtaattct ttactcccca gcgttatcga cgttgcgact
     3301 ggcatgtttt tagattgatc tgctgcctgg ccatcgatga cagaggtaag ataggtgaac
     3361 tgttctgcga ccttgtccat gtgctctgct tcacgctgtt ccatgatctt tgggatgtag
     3421 attgtttgta caagggagat gactgctaca ataagaccga tgagtaatat tgcagtgacg
     3481 acaccgacga ctgcattgtc tgatttttta aattttttca tttgcatcat tgctttggtc
     3541 gttataattt tacttttttt ccgcatttta tgccccttat tgtaagataa taaatttgag
     3601 ttggccccta tttttttcat atctcaattc ctgtttttag taggatttta ttatttcttt
     3661 tattgagtgt aggttacgtc tacttgtacc cagtcgttcc atgtataata gtttttattt
     3721 gtgcttactt ggaaccaaat ttttaaggaa ttaactttag cagcattgtt aatattgcct
     3781 aagagatatt tggtgacaat gacatcggta gtagatggga ttaaagcaat aacttgccag
     3841 ttagagccat tgtagatcat cagtctagca ttggtggtcc cagcggtacc tgtaatattc
     3901 cattcgaatt tgagcgtgac atttatcaca gttgcaccag taggaatatc tgggaagtta
     3961 aactctatac gtttaccaaa attattggtc cattgggcct ggctccggta tttgagatta
     4021 tcacttaaat tcaggttcag gcggttactt ggagttaggg tgtaatcact accagacgta
     4081 ttatcataga caaggttggg tttgagattt atgactgtac ttgttttcgt ggttaatgta
     4141 taccatctgc gagtccacag accactgcca gttggatcgg tcgcattcac ccagattctg
     4201 tacgtcgttg aatgggaaag cccagaaagt gagagtgttt ttgttccatt tgtcgctcca
     4261 gcggcgctgt tggtttgtcc gttgctgcat tgggtggtcc aggtgaaggg atttccctca
     4321 agatcattga tcgggatatt ccagggtaga ttcaatggtt ggtttatcga accatttgat
     4381 ggggtgggtg tcccaaaggc tggtggaagg tttcctgtgg tggtgaaggt gtaccatttc
     4441 cgtgtgaaga ggttactgcc tgttgggtca gtggcattga ccaagatttt gtacgtggtc
     4501 gaatatgaaa gtcctgtaag aatgagtgtt ttttgtccgt ttggtcccgc gccgttattt
     4561 gtttgtccgt tgctgcattg gatggtccag gtgaagggat cgttttcagg gtcgtcgatc
     4621 tggatcttcc aggtaagacc cagcaggttc cctgttgaac cgtttgttgg gagtggtgtt
     4681 gtgatggtcg gtggtttgtt cgtgatgact gaggttgcag gtccatgttg gagtacgact
     4741 gataatatga gcgtgtttgt cgaaggatcc atgactgatg cctggatgat cgtatcggtg
     4801 aagtcgtatc ttgaattgaa ttgtatggtt tccccgaagt cccatttgtc agggtttttg
     4861 tcatccttta aaggcagtag tttccagtcg gttgttccat cgatgatgtc ccgtacggtg
     4921 cttttatttg tcgtcgcccc taccgtgatg attatttctg tgtttccatc aagggattct
     4981 ccgccgttgt gttcaatggt gatattgttg tgtgttccat cagcagagaa tgatccgatg
     5041 agactaacag agggagctgg tgattcaaag gaaaatgaga acacgatgta attgaggaaa
     5101 ccaaataatg caatggttat ccctaacaat aatacggtcg caaggacctc tgatactgcg
     5161 tggtttgacc gtctcttctt tttcattgtt tctccattgt ttagaacgtc aatttaaaga
     5221 tgatgaagga gatgattccc aggatgcagc tgtagcggat tcctgaggat atttttccgt
     5281 ccatcatgaa gccgctaagg atgcctgctc ctatgctttg gacgaacacg aacgagtaga
     5341 gtgagtattt gagctccatg cggtcgactg aggtgaatac gactccagcc atgcgtccga
     5401 cttgttttcc ttgtaagtcg aagaaggatt tgaagatggt gaggttcatg atgatgatga
     5461 tcgcgaggaa gacaaagaag cagacgagga tgacgaccgt gtagatcgac atcgtggctt
     5521 tccgttggtt ttccaatcga ttgacttggc tgacttctat ggctgctgcc ctgaagatct
     5581 ttggtgtgtt tccccccatg accagtcctt tgttcaggct gatgacgatc cgatggatga
     5641 tcgcggattt catccggtct gcgaagtcga tgaggatttc ttttaccgcg atgttccagg
     5701 agagctggga ccgcattttt ttgatttctg tggagagtcg tccgtagtgt gattttgagg
     5761 cgacctttat cgcatcaaag atcgttgcac cggtagacag cgaatcgctt acctcgatga
     5821 ggaagttcgg taatcggtat tggatctgtt tctttttttg tgcctcgaaa taattgtagg
     5881 tgccgatggg gccgatgagc aggaggatgg ctaggaagat gaagtcgagc tgggggtaga
     5941 acgtgaagct gatgtatccg agataatcca tgacgcgaag accaatgtag gatatgacga
     6001 cgatgaggct ggagaggagc aatatcatga tgggtgtttc gttttccttc aggtattgcc
     6061 ggaaggtgag gttttctttg gagtagatcc tgttgaggtc ctcaagactg gtggacctga
     6121 tgaggaaata gaagccagcg tagatcagcg gcatcatcac tagggagaag atgttgagga
     6181 tgacgatgga actgaccgat gaaccgccga aaaaaccaaa gaccgtgagg atgatgacca
     6241 gaaaaattgg gaatgcgatg gcgcacgtga cgaagatttc tgcgatgacg ccgagcaagt
     6301 ccaggtctt
//
LOCUS       DATMXJ010000087         7574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12162, whole genome shotgun sequence.
ACCESSION   DATMXJ010000087 DATMXJ010000000
VERSION     DATMXJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12162"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="carA"
                     /locus_tag="VN377_02515"
     CDS             complement(<1..1055)
                     /gene="carA"
                     /locus_tag="VN377_02515"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWR27291.1"
                     /translation="MEPRKYARLVLEDGSVFSGYSFGTHCSVSGEVVFNTGMVGYPET
                     LTDPSYKGQIVTLTYPLIGNYGVPGTEKEDGLLRFFESEKIQVEGIIISDYSDRYYHW
                     NAKKSLSAWLQEYNIPGIYGVDTRALTKKLRTKGTLLGKIIHDHQKKVFHDPNEKNLA
                     AAVSIKEPVTYTKGKRTILLVDCGAKNSIIRAFLKRNFTVKRVPWDYDFTSEAVDGIV
                     LSNGPGNPKKCDETITHVTTALAGDIPILGICLGSQILALAAGADTYKLKYGHRSQNQ
                     PCMEAGTNRCYITPQNHGYAVDADSLPQDWREWFYNGNDQTNEGIKHISKPFFGAQFH
                     PEASPGPDDTEFLFDLFV"
     gene            complement(1128..3116)
                     /locus_tag="VN377_02520"
     CDS             complement(1128..3116)
                     /locus_tag="VN377_02520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF018444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWR27292.1"
                     /translation="MAELFPYQPRQNQKEIMQEISEALRQKKQFIFESGTGSGKTICV
                     LAAALAYALEHHKKIIYTTRTNAQQQQVIHELRAIQKKSQDKRIYGVGMQGRGNMCLL
                     ARENPEVQNGNAEELSRFCSYQKKLAVSEKRKGCGYYRSYLGEKDRVETVMEWAQNNL
                     PTAEEFTDICEKQGLCPYEINKLIVHEALVVVVPYVYVFDKSIRIKLFDWLSISEEDV
                     ILVVDEAHNLPDYLRELFSSELSTWMLNSCLMEAEQFGNPSLADGRISVTAFCKEIMD
                     IVTVLRDTYVYGVLEGGIRRINPEKNDALIPSHELEVEILSRLKTTSKTLRDIISDLF
                     AYGEKIQEYKQKDGKLPRSYLHNLGVFLGFWTTIEMDQYIKLVVDTGQGKNPRVEAYC
                     LDPSIGTDVLKEVHCSIHMSGTLEPLDEYRDSLGLPLETRMALYPSPFPPENRRIFFV
                     RDVTTRYDELTKDKTIIPQMWRYVSEICNRFEKNTMVSFPSYNTLAMFQKNGYFAEIQ
                     RCLYIEEQHMSQSELMDLVSEFKSCGDGNGNRAALFTVMGGRISEGMDFPAEQLEIAV
                     IVGIPYPKPTARQRGLQNYYDQKFGKGWEYTVQAPAARKMLQAIGRLIRNENDRGVAV
                     ILDKRAPRFHQYIKEMRESKNLLMDINRFMNQQRTMEI"
     gene            3206..4048
                     /locus_tag="VN377_02525"
     CDS             3206..4048
                     /locus_tag="VN377_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HWR27293.1"
                     /translation="MLETPFKSIKRTVSSFIPSELVSHLPSKWEKIGSVVTIKVPDEL
                     SDYKEIIGKVYAEGLDCKTTLNEKGGIVGVYRMPVVEVIFGSSDTETIHVENGVRFKV
                     DPQQIMFSSGNMAERQRMATISDPYETVVDLFAGIGYFTLPIAVYSKPKKIVACEINP
                     VAFKYLCANVVLNHVSGIVEPCFGDNREVAPKDCADRVILGYLHEPQDFLPVAFRCLK
                     NHIGVLHYHELVPVESIPRQPLSHIEQVAKRFDRRVDLLKVNLVKSYAPRVNHLVLDV
                     RISE"
     gene            4045..4560
                     /locus_tag="VN377_02530"
     CDS             4045..4560
                     /locus_tag="VN377_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HWR27294.1"
                     /translation="MKKPVLLVVYHANQDDPRKCTAKKLHRFGFATLQKNVRRIPSHA
                     ILLDPFAEKSLSCEDIAVAQRNGLVALDCSWKTAETMFQSLELRMIPRALPFLLAANP
                     VNYGKPYQLSTLEAFAAALYILDEVVQATAILGIYTWGHRFLELNKEPLEEYREARSS
                     AEVIQIMNKYI"
     gene            4618..6042
                     /locus_tag="VN377_02535"
     CDS             4618..6042
                     /locus_tag="VN377_02535"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWR27295.1"
                     /translation="MSEKPKNEREEDGMKIPLETFFKPKSIAVVGASKNPTKIGHASL
                     KNILISDYSCKLFPVNPNEEEILGLKCYKQLTDIPDLVDLVMIAVPEPLVLQIIKDCV
                     KKKVKWVIIISSGFSEIGNHEGEEAIKKLVDGTGIRILGPNTMGYKNASDNLDVSFVF
                     GMPRKGNLALISQSGALGMGMIYLANNEFVGLSKIIGVGNKLDIDDDDLVDYFSQDPE
                     TKVIGLYIEGIRHGRDFMNSIKACNKPILVVKAGRSTAGARATASHTGSMAGSDELYS
                     AAIKQAGGIRCRDLVELFDMARALDGQPAARGNRIGIITNGGGLGILLTDACEANGLT
                     VPKLSQKTFKKIEKIVPGLVKPNNPVDLVADAGFYRYWASTRALLEDENIDGIIVASV
                     HAGYARPREYTGAILKMIHQQKLHHEYEKPILGCWVGGKEFEDLVLDLKAVGVPIYPS
                     SWRAARAMLALSLEGERLKREQKI"
     gene            complement(6039..6323)
                     /locus_tag="VN377_02540"
     CDS             complement(6039..6323)
                     /locus_tag="VN377_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27296.1"
                     /translation="MNKDLKEWISEEDQTDISETIHARLKDKKPQGKCTICRVHPAKS
                     ICIKCGDSVCSSCFFTMVGLCQNCLSKETIEKWKNKKTDWKKILDVDWVD"
     gene            complement(6417..7022)
                     /locus_tag="VN377_02545"
     CDS             complement(6417..7022)
                     /locus_tag="VN377_02545"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HWR27297.1"
                     /translation="MDYTVIIQAFWIVIPIYIANASAVIVGGGTPIDFGRSWKDGRRI
                     LGDGKTWRGLFAGAFLGMTAGFGLAVAASYLSASEYSFLGLTNFEGFPLMIPILFSLC
                     FGALLGDIIESFFKRRIGKDRGQDWIPFDQLDFIVGALIFSFLISELLYVTHVTDVHW
                     FFIHITPWHILILLIVTPFIHITANILFKKIKKKQAKNLRI"
     gene            complement(7068..>7574)
                     /locus_tag="VN377_02550"
     CDS             complement(7068..>7574)
                     /locus_tag="VN377_02550"
                     /inference="COORDINATES: protein motif:HMM:NF021353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HWR27298.1"
                     /translation="VINDSTDLVSIIRAFDSPTKKEVFMEISKDWIPCSIILGKFGED
                     GQAALEFFDKMKLAETKWTTPEAGIDGKPQKMYRSFYSLFNINISCPVNEISEIFNVS
                     SLNDFQFKKLEEDIFEFIGDTGTFANKVSEKFGISNTALKALVKRSNKFQYTGLKITR
                     TQPGPEST"
BASE COUNT         2047 a         1660 c         1723 g         2144 t
ORIGIN      
        1 acaaacagat caaataaaaa ctcagtgtca tcaggaccgg gggatgcttc cgggtgaaat
       61 tgtgctccga agaatggttt agagatatgt ttaatgcctt cgttcgtctg atcattacca
      121 ttgtagaacc attcacgcca atcctgtggt aaggagtctg catccacggc gtacccatgg
      181 ttctgcggag tgatgtaaca tcgattcgtc ccggcctcca tacaaggttg attctgactt
      241 cgatggccat atttgagttt gtaggtatct gcaccagcag ccaacgctaa gatctgagaa
      301 ccaagacata tcccaagaat cgggatgtct ccggctaacg cagtagtcac atgggtaatt
      361 gtctcgtcac atttttttgg gtttcctgga ccatttgaca gcacgatacc atcaaccgct
      421 tctgaggtga aatcgtaatc ccagggaaca cgctttactg tgaaattccg tttcaagaac
      481 gcgcgaataa tgctgttttt cgccccacaa tcaacaagga ggatggttcg ttttcctttc
      541 gtataggtca ctggttcttt gatgctgacg gccgcagcaa ggtttttttc gtttggatca
      601 tggaagactt tcttttgatg atcatgaatt atttttccta aaagtgtgcc ttttgtccgt
      661 aatttttttg tcagtgccct tgtgtccact ccatagatac ctgggatgtt gtattcttgt
      721 agccatgccg ataatgactt ttttgcgttc caatgatagt accggtcaga gtagtccgaa
      781 ataatgattc cttcgacctg tattttttct gattcaaaaa accgtaacag accatcttct
      841 ttttcagttc cagggacacc atagttaccg atcaaaggat acgtcagggt gacgatttgc
      901 cctttatatg agggatccgt cagggtttca ggatagccaa ccatcccagt gttgaacacg
      961 acttctccac tgacggaaca gtgtgtgcca aatgaatatc ctgaaaaaac agagccatcc
     1021 tctagtacaa gccttgcgta tttccttggt tccatccaat gaccacctca cccatcacgt
     1081 gaatgatact ttcgtattct gtatatcgtg acgtcttaaa gaaattccta gatttccata
     1141 gttctttgtt gattcataaa acgattgata tccatgagta aattttttga ttcccgcatc
     1201 tcttttatgt actgatggaa tcgcggtgcc cgtttatcga ggatcaccgc gaccccacga
     1261 tcattctcat tgcggatcaa acggccaatt gcttggagca ttttccgtgc agcaggtgct
     1321 tgcacggtgt attcccagcc tttgccgaac ttttgatcat aatagttctg caaacctcgt
     1381 tgacgtgctg ttggttttgg ataaggaatc ccgacaatca ccgctatctc gagctgctca
     1441 gcagggaaat ccatcccttc gctgatacgt ccacccatga cagtaaaaag agcagcgcga
     1501 ttcccatttc cgtccccgca ggatttaaac tcactgacca aatccatcag ttctgattgt
     1561 gacatgtgtt gctcttcgat atacaagcaa cgttggattt ccgcgaaata cccgttcttc
     1621 tgaaacatcg caagggtgtt ataactaggg aaggaaacca tggtgttttt ctcgaaccgg
     1681 ttgcaaatct cgctgacata tctccacatc tgagggatga tggttttatc tttggtcagc
     1741 tcgtcatatc gtgtggtgac atcgcgaaca aaaaaaatcc tccgattctc gggagggaac
     1801 ggggatggat atagcgccat ccgtgtttcc aacggaaggc caagtgagtc gcgatattca
     1861 tctaacggtt caagcgtccc agacatatgg atgctacaat ggacttcttt aaggacgtcg
     1921 gtgcctatcg atgggtcaag acaatacgct tctactctcg ggtttttccc ttgaccggta
     1981 tcgacgacga gtttgatgta ttggtccatt tctatggtcg tccaaaagcc gaggaacact
     2041 ccgagattgt gaagatacga gcgtgggagt tttccgtctt tctgtttata ctcctggatt
     2101 ttttcgccat atgcgaagag gtcactgatg atgtccctta gtgttttact ggttgttttg
     2161 agacgtgaga ggatttctac ctcgagttca tgggaaggga tgagcgcatc gtttttctct
     2221 ggattgatcc ttcggatacc accttcaagc accccgtaga catacgtatc ccgcaagacg
     2281 gtgacgatat ccatgatttc tttacaaaac gcggtcacgg atattcttcc atcggcaagt
     2341 gaaggatttc caaactgttc tgcttccata aggcagctgt tcagcatcca ggtgctcagt
     2401 tctgatgaga ataactctcg gagataatcg ggaagattat gcgcttcatc gacgacaagg
     2461 atgacgtctt cttcagagat ggataaccag tcgaaaagct tgatgcgtat cgatttgtca
     2521 aatacataga catagggaac gacaacaaca agagcttcat ggacaataag tttattgatt
     2581 tcatacgggc agagcccttg tttttcgcag atatcggtga attcctctgc ggtcggtagg
     2641 ttgttctgtg cccattccat gaccgtctct acacggtctt tttcaccaag gtaacttcga
     2701 tagtagccgc atccttttct tttttctgat accgcgagtt ttttttgata ggagcagaac
     2761 cgggagagct cctcggcatt cccattctgg acctctggat tttcacgtgc gagcaagcac
     2821 atgtttccac gaccttgcat cccgacgcca tagattcttt tatcctgaga ttttttttgt
     2881 atcgcacgaa gctcatggat cacttgttgt tgttgcgcat tcgtccgtgt cgtatatatg
     2941 atttttttgt gatgttccaa tgcataggcg agtgctgcag caaggacaca gatggtcttg
     3001 cctgaaccag tccctgattc gaagataaac tgtttttttt gtctgagtgc ttctgagatt
     3061 tcctgcatga tttctttttg attctgtcgt ggttgataag gaaataactc cgccatccat
     3121 ttagcgaata cgctgtagag tacatagact tgttggagtt gagagggatg aagttttata
     3181 ggggagtagt atgtaagaga aggagatgct ggaaacgcct ttcaagagta tcaaaagaac
     3241 tgtttcatcg ttcattccat ctgaactggt cagtcatctt ccaagcaaat gggaaaaaat
     3301 cggttctgtg gtgacaatta aagtacctga tgagctgagt gattataaag aaatcattgg
     3361 aaaagtgtac gctgaggggc ttgattgtaa gacgacgttg aacgagaaag gggggattgt
     3421 tggggtctat cggatgccag ttgttgaggt gatctttggt tcatctgata ctgaaacaat
     3481 ccatgttgag aatggtgttc gtttcaaggt tgacccgcag cagatcatgt tttcctcggg
     3541 gaatatggcg gagcggcaac gtatggcaac catttcggat ccttatgaaa ccgtggtcga
     3601 tttattcgca gggatcgggt attttacgct cccgattgcg gtgtacagca aaccaaagaa
     3661 aatcgtcgcc tgtgagatca accctgttgc gttcaagtat ttatgtgcaa atgtggtttt
     3721 gaatcatgtc agtggcattg tcgaaccatg ttttggtgat aatcgagagg ttgccccgaa
     3781 ggactgtgca gatcgagtga tccttgggta tctacatgaa ccgcaggatt ttttaccggt
     3841 tgcttttagg tgtctgaaaa atcatatcgg ggtgcttcat tatcatgagc ttgtacctgt
     3901 tgaatcaatc ccacggcagc ctctttccca tatcgaacaa gtggcaaaac ggtttgatcg
     3961 tcgtgtggat ttactcaagg ttaatctggt caaatcgtat gctcctcggg tgaatcatct
     4021 ggtgcttgat gtgaggatct cagaatgaaa aaaccggttt tgcttgtcgt gtaccacgcg
     4081 aatcaggatg acccccggaa gtgtacggca aaaaaattac atcggttcgg gtttgcgacc
     4141 cttcaaaaaa atgtccgtag gataccttct catgcgattc tcctcgatcc ttttgcagaa
     4201 aaaagcctgt cttgtgagga tattgcggtt gcccaaagaa atggtcttgt tgcgttggat
     4261 tgttcttgga aaaccgctga aacgatgttt caatctctag agctgcggat gattcctcgt
     4321 gcgttaccgt ttttgcttgc agccaatccg gtgaattatg gaaaaccata tcagttgagc
     4381 acattggaag cgtttgcggc agcgttatat atccttgatg aggtagtgca ggcgaccgcc
     4441 attcttggga tttatacgtg gggtcatcgg tttttggagt taaataaaga gcctttggag
     4501 gaatatcggg aggctcgttc gagtgctgaa gtgattcaga tcatgaataa atatatataa
     4561 aaagatattc agaagtatat ctaatttatt tcagtatgtt taagtaaaga gaagtaaatg
     4621 tctgaaaaac caaaaaacga aagagaggaa gatggaatga aaataccact ggagacattt
     4681 ttcaagccaa aaagcatcgc ggtcgttggt gcttcaaaaa acccaacaaa aatcggacat
     4741 gcatcactga aaaacatcct catctccgac tactcctgta aattatttcc tgtaaatcca
     4801 aacgaagaag aaatccttgg attgaaatgc tacaaacaac tcaccgacat cccagactta
     4861 gtggaccttg tgatgatcgc agtacccgaa cctcttgtgc tacaaatcat aaaagattgt
     4921 gtgaaaaaaa aggtcaaatg ggtcataatc atctcctcag gattcagcga aatagggaac
     4981 cacgaaggag aagaagctat aaaaaaactg gtggatggaa ctggaatccg aatcctagga
     5041 ccaaatacca tgggatacaa aaacgcatct gataaccttg atgtctcctt tgttttcggt
     5101 atgcccagaa aaggaaacct tgccttgatc agccaaagtg gagctctggg tatggggatg
     5161 atctatctgg cgaacaacga gtttgtcgga ctgtcaaaaa tcatcggcgt gggaaacaaa
     5221 ctcgacatcg atgatgacga cctcgtcgat tacttcagcc aagacccaga gacaaaagta
     5281 atcggactgt acatcgaagg gatacgacac ggacgagatt ttatgaacag catcaaagca
     5341 tgtaacaaac ctatcttggt ggtcaaagct ggaagaagca ccgcgggcgc cagggcgacc
     5401 gcatcccata ctggttctat ggcaggaagc gacgagttgt acagcgctgc aatcaaacaa
     5461 gcaggcggga tccgttgccg agacctcgtc gaactcttcg acatggcacg agcacttgat
     5521 ggacaaccag cagcccgagg aaatcgtatc gggatcatca ccaacggtgg tggattgggg
     5581 atccttctca ccgacgcctg tgaggcaaat gggttgaccg tcccaaaact ctcccaaaaa
     5641 accttcaaaa aaatcgaaaa aatcgtccca gggctcgtca agccaaacaa ccctgtcgac
     5701 cttgtcgcag acgcagggtt ctaccggtac tgggcatcca cccgagcact ccttgaagat
     5761 gaaaacatcg atggaatcat cgtcgcatca gtccacgcag gatatgcacg accccgagag
     5821 tacacaggcg ccatcttaaa aatgatccac caacagaaac ttcatcatga atacgaaaaa
     5881 ccaatccttg gctgctgggt gggcggcaaa gaattcgaag acttagttct tgaccttaaa
     5941 gctgttggag tccctatcta cccatcatcc tggagagcag ctcgggcgat gcttgcgtta
     6001 tccttagaag gagaacgact caagagggaa caaaaaatct aatccaccca atcgacatca
     6061 aggatcttct tccaatccgt ttttttattt ttccatttct caatcgtttc tttcgaaaga
     6121 cagttctgac acaacccaac catagtaaaa aaacaagagc tgcacaccga atccccacat
     6181 ttaatgcaga tggattttgc aggatgcacc cgacagatgg tacatttccc ctgtggtttt
     6241 ttatccttca atcgagcatg gattgtttct gagatgtctg tctggtcttc ttctgagatc
     6301 cattccttca aatccttatt catagtaata gttgtatcct tattcgatgt tttttttaat
     6361 gaggtttttt ttggacacat actcccgcga gaaccaagta taaaaagaga ttctcatcag
     6421 attcttaaat tttttgcttg tttcttcttt atttttttaa aaagtatatt tgcagtgata
     6481 tggataaacg gggtgacaat cagaagaatc aggatatgcc atggagtgat atgaatgaaa
     6541 aaccaatgca catctgttac gtgtgtgaca tagagaagtt cgcttatcaa aaaactgaaa
     6601 atcagtgccc cgacgatgaa atcaagttgg tcaaatggga tccaatcctg accacggtcc
     6661 ttaccgatac gtcgtttgaa gaaactctcg atgatatccc caagtaatgc cccaaagcag
     6721 agggaaaaaa ggatcggtat catcaggggg aatccttcga agtttgtgag tcctagaaag
     6781 ctgtactcgc ttgcggagag gtaggaggca gcaactgcaa gaccaaagcc tgcggtcatc
     6841 ccaaggaagg ctcctgcgaa caatccccgc caggtttttc catctcctag gatccgacgt
     6901 ccatccttcc aactccttcc aaaatcaatc ggcgtcccac caccgactat cactgcgcta
     6961 gcgttcgcta tatagatagg aatcacgatc cagaacgctt gaataatgac agtgtaatcc
     7021 attggggctg gtatcgttca atggcttaaa accattgtga aaaaaaatca agtgctctca
     7081 ggtcctggtt gggttctggt gattttcaac ccggtgtatt ggaacttgtt tgatcgtttt
     7141 actaacgctt ttaaggcggt gttggagatc ccgaatttct ctgagacctt gtttgcgaat
     7201 gttcccgtgt cccctatgaa ctcaaagatg tcttcttcga gtttcttgaa ctgaaaatca
     7261 tttaaagatg agacgttgaa aatctcactg atttcattga ccgggcagga gatgttgatg
     7321 ttaaacagag agtaaaagga acggtacatc ttctgtggtt tgccatcgat gcccgcttcc
     7381 ggcgtcgtcc atttggtttc tgccagtttc attttgtcaa agaattcaag ggcagcttga
     7441 ccgtcttcac caaacttacc aaggataata ctacatggta tccagtcttt tgatatctcc
     7501 ataaaaactt ctttttttgt gggcgaatca aatgctctga taatagagac caaatcagtt
     7561 gaatcgttta tgac
//
LOCUS       DATMXJ010000088         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_64608, whole genome shotgun sequence.
ACCESSION   DATMXJ010000088 DATMXJ010000000
VERSION     DATMXJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64608"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..619)
                     /locus_tag="VN377_02555"
     CDS             complement(65..619)
                     /locus_tag="VN377_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HWR27299.1"
                     /translation="MRVALSGTPGTGKTSVATALKRHGYSIVHLHAFAKENHCIAGVD
                     TKRDSQLIDMVKLDKAIQKNFTTDALVFFDGHIGHILNTMDKVILLRCHPKELEKRLL
                     KKKWSAKKIRENVDAETIDIILCEAVERHPSDDIFEIDSTKKTVEEIATVVIGFVKKN
                     FQPTRTYRIGQIDWSEEIIHRDHM"
     gene            complement(621..1916)
                     /gene="glyA"
                     /locus_tag="VN377_02560"
     CDS             complement(621..1916)
                     /gene="glyA"
                     /locus_tag="VN377_02560"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWR27300.1"
                     /translation="MKWYDEVEYIRKQAMKNNEFFANSLPMIASENVLSPLCREMLIT
                     DFHGRYAEGTPGNRYYEGCKYFDRVEEKTMELAKKLFSCRYADVRPTSGTVANMAVLK
                     ALIKPGETATVLDTANGAHISFGKYGAAGVRGINLVSFPFSNEEMVIDVDGAVKLIRQ
                     VKPKLALCGQSVFLFPTPLKAIAEAAHEVGAYVVYDAAHVLGLIAGKRFQDPLREGAD
                     VMNGSTHKTLPGPQGGMILSDHDPDSEVEKAFLRKLGFGVFPGVTSSYHLHHVAAKGI
                     AFAEHLEFGEAYADQTIKNAKRLAQALYEQGFKVFGEKLGFTKSHQVLVEIGLGKGKE
                     ASKILEDGGIVTNMNMIPGDVDPLNPSGLRLGTPELTRIGMKEKDMDDVAVFYARVLL
                     KKESPKKIRDDVKEFRKDFQELHYCYKEGFRGYDYTKLI"
BASE COUNT          542 a          564 c          384 g          592 t
ORIGIN      
        1 ttcgcaatca ccgtaagcaa aggatccgct ctctcacgtg ttctatccaa taccatatct
       61 actcctacat atgatcccga tgaatgatct cctctgacca atcaatctga ccaatcctat
      121 atgtccttgt cggttgaaaa ttttttttca caaaccctat gacgactgtg gcgatttctt
      181 caacggtctt cttggttgaa tcaatctcaa agatatcatc tgaaggatga cgttcaaccg
      241 cttcacaaag gatgatatca atggtttccg catcaacatt ttcccttatt tttttagcac
      301 tccatttttt tttaagaagc cttttttcaa gttccttagg atgacaacgc agaagaatca
      361 ctttgtccat tgtgttcaga atatgcccga tatgcccgtc aaaaaaaaca agagcatccg
      421 tagtgaagtt cttctgaatt gccttgtcca acttcaccat atcaataagc tgggagtctc
      481 ttttcgtatc aacccccgca atgcagtggt tctcttttgc aaacgcgtgc aggtgaacaa
      541 tgctgtaccc atgtctcttc agagccgtcg caaccgaggt ttttccggtt cctggggtac
      601 cggaaagtgc caccctcata ttaaatcaac ttagtataat catatccccg gaacccttcc
      661 ttatagcaat aatggagttc ctggaaatcc ttacggaatt ctttgacatc atctctgatt
      721 ttcttcggag attctttctt cagcaacact cgagcataaa agaccgcaac atcgtccata
      781 tccttttcct tcatcccgat acgagtaagc tcaggcgtgc ccaatcggag cccagaaggg
      841 ttcaacggat cgacatcacc agggatcata ttcatgttcg tgacaatccc tccatcctca
      901 agtattttcg aggcttcctt ccccttccca agaccaatct ccacaagaac ctgatgggat
      961 ttcgtaaaac caagtttctc cccaaacacc ttaaaccctt gttcatagag tgcctgagca
     1021 agtcgtttcg cgttctttat cgtttgatct gcgtacgcct ccccgaactc aagatgctcg
     1081 gcaaaggcga ttcctttcgc agccacatga tgcagatgat atgaggaggt aacaccaggg
     1141 aacaccccaa atccgagttt acgcaggaac gctttctcaa cctcactatc cgggtcatga
     1201 tctgagagaa tcatcccgcc ctgtgggcca ggcaatgtct tatgggtgct tccattcatc
     1261 acatcagcac cttctcgcag tggatcctgg aaacgtttcc ctgcgatcaa accaagcaca
     1321 tgagctgcat catacacaac gtacgcaccg acctcatggg ctgcctcggc gatcgccttc
     1381 agcggagtcg gaaacagaaa aaccgattga ccacacaacg ctagctttgg ttttacctgc
     1441 cgtatgagtt ttactgctcc atccacatca ataaccatct cctcgttact aaacgggaat
     1501 gagacaagat taatccctct gactcctgcg gcaccatatt tcccaaacga gatatgagca
     1561 ccattcgcgg tatcaaggac cgttgcggtc tcccctggtt tgatcagcgc cttcaacaca
     1621 gccatattcg caaccgtccc tgaggtcggt cgcacatcgg catacctgca agagaataat
     1681 ttttttgcaa gttccattgt tttttcttcc acgcggtcaa agtatttaca tccctcataa
     1741 taccgattcc ccggggtacc ttccgcgtat ctcccgtgaa aatctgtaat caacatttcc
     1801 cgacatagcg gagacaggac attctcactg gcgatcatcg gaagagaatt tgcgaaaaac
     1861 tcgttatttt tcatcgcctg tttcctgata tattcaacct catcatacca tttcatagaa
     1921 tcaaccaaag ctgtatcttt gacttcgata aaaaggtaat ggaaaggttc ttttaaagat
     1981 gcgaagacct attttttctt ttcttcccgc attttaaata aggacgggag atggatcgta
     2041 tacgttccat ccttctggat cacgattggt tctctctcca gg
//
LOCUS       DATMXJ010000089         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_22510, whole genome shotgun sequence.
ACCESSION   DATMXJ010000089 DATMXJ010000000
VERSION     DATMXJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22510"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VN377_02565"
     CDS             complement(<1..309)
                     /locus_tag="VN377_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWR27301.1"
                     /translation="MDDLQRLEELDELLIELREENKIVPIIVEGEKDIIALRRLEMTG
                     EIIRFNTGQSVPGFCDVIAQKYQKIILLTDWDWRGGRLGFSIKKHLENRVECNMNYRQ
                     M"
     gene            complement(302..622)
                     /locus_tag="VN377_02570"
     CDS             complement(302..622)
                     /locus_tag="VN377_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWR27302.1"
                     /translation="MTSYKDAIKDSPQGVLLFLHVIPGSAQTVFPVQYNQWRQSIEMK
                     VRSEAKDNKANIEVVETIARFFQLAGKDVVLVNGEKKREKTVCLKKISATTVDRTIKE
                     YLDG"
     gene            complement(644..994)
                     /locus_tag="VN377_02575"
     CDS             complement(644..994)
                     /locus_tag="VN377_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HWR27303.1"
                     /translation="MMPGKMNSRQLAQMMKRFGINVKNIDNVEKVLIQTDTKEYVFDS
                     AEVTVMEAQGQKTFQIIGEPIVKNRGAETQDTQKEDVKLIMEQTGKSEAEVRKALKET
                     NGDLAEAILKLTQP"
     gene            1059..2510
                     /locus_tag="VN377_02580"
     CDS             1059..2510
                     /locus_tag="VN377_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWR27304.1"
                     /translation="MISEAEMKVIDRNSEYFGVPTLQLMENAGRNISEFVTNIIKDTK
                     KNILILCGTGNNGGDGFVAARYLTQYYKVSVLLAGPDIKTEIAKKNFQRLQTYEVKIY
                     TSLKDLDNLLTENDVLIDALLGTGLAGELKEPYMTIVKKINETQKKIIVSVDVPTGLG
                     STHKITPDHTVTFHDRKEGMNEKNSGKITIVDIGIPQEALTYVGPGELSVYYPRPAKK
                     SHKGENGIVLVIGGGPYIGAPALSGLAALRTGVDLVYIATPQRSWESIATFSPNFIVK
                     DLAHDMLTKEDLPQIEQLLGKCTGVVLGPGLGTASETEEAIIPLVTRIIAEKKPLILD
                     ADALKPVGENLALIRNTTTVVTPHVGEFKKLTGITLSQDLDIRIAVVNEWAAKLGITI
                     FLKGYIDVLSNGTDFRQNKVHNEAMTVGGTGDVLAGIIGALLSKGVRPFEAVRIAAFL
                     NGAAGNEAFQKKSYGLLATDIIEEIPNVLKKYL"
     gene            complement(2557..2847)
                     /locus_tag="VN377_02585"
     CDS             complement(2557..2847)
                     /locus_tag="VN377_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27305.1"
                     /translation="MWEKKSSGMFVKKTEKAVRKIADRKTEMIEQTAEWMKKYQYAEW
                     PKNPDKLEQEMKNKITTALSEEMTHEPAFLAWVLYQVAGDLVKHQEVKAIWE"
     gene            2944..>3231
                     /locus_tag="VN377_02590"
     CDS             2944..>3231
                     /locus_tag="VN377_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27306.1"
                     /translation="MKATYLLIGIIIIFSLMPPSATAFFTSSHTLRATLTEDTEVFFI
                     GKTSIDGSFTGLPMDHLVDSPLIQDMGGFPLIGKSRMTNLDSVIVAEDIDIT"
BASE COUNT          899 a          777 c          640 g          915 t
ORIGIN      
        1 catttgacga taattcatat tacattctac acgattctcg agatgtttct taatagagaa
       61 acctagccgt cccccgcgcc aatcccaatc cgtaagaaga ataattttct ggtatttctg
      121 ggcgataacg tcacaaaaac ctggcacgga ttgtcctgta ttgaatcgga tgatctcacc
      181 agtcatctca agcctgcgta atgcaatgat atccttctcc ccttcgacaa taattgggac
      241 gattttattc tcctcccgta gctcaatcaa tagctcgtcg agttcttcta atctttgaag
      301 atcatccatc aagatactcc ttaatcgttc tatccactgt agttgcagag attttcttca
      361 gacagactgt tttttccctc tttttttccc catttacaag cacaacatct ttccctgcga
      421 gctgaaaaaa ccgtgcgata gtctcaacga cctcgatgtt tgctttgtta tccttcgcct
      481 ccgatcggac cttcatttca atggactgtc gccattggtt atactgaaca ggaaacaccg
      541 tctgggcgga ccctgggatc acatgcaaga aaagaagcac accctgcggt gaatctttga
      601 ttgcatcctt atacgaagtc atagtccctt ataaaacagt tgattatggt tgagtcagct
      661 tcaggatagc ttcggcaaga tccccattcg tctcctttaa agccttccgc acctctgctt
      721 ctgatttccc tgtttgctcc atgattaatt tgacatcctc tttttgagtg tcttgggttt
      781 ctgcccctct gtttttcacg attggttccc cgatgatctg aaacgtcttc tgtccctgcg
      841 cctccatcac tgtgacttca gcagaatcaa acacatattc ctttgtatct gtttgaatca
      901 gaactttttc tacgttatcg atgttcttga cgttaatccc gaatcgtttc atcatctgag
      961 caagctgcct actattcatc ttacctggca tcatagataa caaatccttc gatggttccg
     1021 aagcgtatat agagttctat tcgtttaaca tttttggtat gatttcagaa gcggaaatga
     1081 aagtcatcga tcgcaactca gagtactttg gcgttccaac attacaacta atggagaacg
     1141 ccggaagaaa tatttccgag tttgtaacga acataatcaa agacacaaag aaaaacatcc
     1201 tcatcctttg cggaacaggt aacaatggtg gagatgggtt cgtcgccgcg cgatacctca
     1261 cccaatatta caaggtcagt gtgcttcttg caggacctga tattaaaaca gagattgcaa
     1321 agaaaaattt tcagagactc cagacttatg aggtaaagat ctatacctca ctcaaagatc
     1381 tcgataatct tctcacagaa aacgatgttc tcattgacgc gctcctcgga actggattgg
     1441 ctggtgagct aaaagaacca tatatgacca tcgtgaaaaa gataaacgaa acacagaaaa
     1501 aaatcattgt ctcggtggat gtccccaccg gtctaggctc cactcataaa atcactcctg
     1561 atcacacggt cacatttcat gatagaaaag aagggatgaa cgaaaagaac agcggaaaaa
     1621 tcactattgt tgatatcggc attcctcaag aggcactcac gtatgttggg cctggtgagc
     1681 tttctgtgta ctatccgcgt cctgcgaaaa aatcacacaa aggagagaat ggaatcgtcc
     1741 ttgtcatcgg tggaggtccg tatatcgggg caccggcttt aagcggtctt gcagcgttac
     1801 gaaccggtgt ggaccttgtc tatatcgcga caccacaacg ctcctgggag tctatcgcta
     1861 ctttttcacc aaacttcatt gttaaagatt tagcacacga tatgctcacc aaagaggatc
     1921 tccctcaaat cgaacaactc cttgggaaat gcaccggggt ggttcttgga ccaggattgg
     1981 gaaccgcatc tgaaactgag gaggcaatca tcccgttagt aacacgcatc atagcagaaa
     2041 aaaaaccatt gatcctcgac gcagatgctc tcaaaccagt cggagaaaac cttgctctta
     2101 taagaaatac aacaaccgtg gtcacccctc atgtaggtga gttcaaaaaa ctcacaggga
     2161 tcacactttc acaagatctt gacatacgga ttgctgttgt gaacgaatgg gcggcaaaac
     2221 tcgggataac gattttcctg aaaggataca tagacgttct gagcaacggg acagacttca
     2281 gacaaaacaa ggtccataat gaagcgatga ccgtcggggg gactggagac gtcctggcag
     2341 gcatcattgg agcgctcctc tcaaaaggag tgcgaccatt tgaggcggtc cgaatcgcag
     2401 cattcctcaa tggggcggcg ggaaacgagg cgttccagaa aaaatcctac gggttactcg
     2461 caaccgatat catcgaagaa atcccaaacg tcctgaaaaa atatctataa tttttatatt
     2521 tttaacaaaa ccgacaagat agagagatga tcatgattac tcccatatcg cttttacttc
     2581 ctgatgcttc acaagatccc cggcgacttg gtacagtacc catgcaagga acgcaggctc
     2641 gtgggtcatc tcctcagaaa gtgctgtcgt gatcttattt ttcatctctt gttcgagttt
     2701 gtcagggttt tttggccatt ccgcatattg gtatttcttc atccattccg cggtctgttc
     2761 aatcatctca gtcttcctat cagcaatctt ccggactgct ttctcagttt ttttcacaaa
     2821 cattccagat gatttctttt cccacatgat tccatcctca tataagatat ctccgtatat
     2881 gataaaagtc tttactttgc aatgaaaaaa cttttaagaa cccaagcgac atcagcggtt
     2941 cagatgaaag caacgtatct gctcatagga atcattatca tattttcctt aatgcctcct
     3001 tcagcaaccg ctttttttac atcgtcgcat acccttagag caacccttac cgaggatacc
     3061 gaagtgtttt ttatcggaaa aacctcaatc gatgggtcgt tcaccggtct tccaatggat
     3121 cacttggttg attctcctct tattcaggac atgggtggat ttccgcttat tggaaaaagt
     3181 aggatgacga atcttgattc ggtcatcgtt gcagaagaca tcgatattac c
//
LOCUS       DATMXJ010000090         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_17677, whole genome shotgun sequence.
ACCESSION   DATMXJ010000090 DATMXJ010000000
VERSION     DATMXJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17677"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VN377_02595"
     CDS             complement(<1..622)
                     /locus_tag="VN377_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWR27307.1"
                     /translation="MGKKEQILRTENLNLWYGEKQALFDITLGISPNKITALIGPSGC
                     GKSTLIRCFNRMNDVIDKCKVTGEVYFHENNIYEPDADAVEIRKKIGMVFQKPNPFPK
                     TIYENVAYGSKVQGITDKKKLDEIVEKSLKAAALWDEVKDRLYESAMGLSGGQQQRLC
                     IARSLSIEPEVILMDEPCSALDPIATAKIEDLMTGLKRKYTVIIVTH"
     gene            complement(630..>2424)
                     /gene="pstA"
                     /locus_tag="VN377_02600"
     CDS             complement(630..>2424)
                     /gene="pstA"
                     /locus_tag="VN377_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWR27308.1"
                     /translation="PIFQKEGVVNFFIGMRWYPTGEPPLYGAFPLIIDTLLVTLGAMV
                     IAIPLSIGSAIFIAEIATPRIKAIIKPAIELLAGIPSVVFGFFGLLVLTSWLQTTFGV
                     PSGESWLAGSILLGIMAIPTITSVAEDAISSVPREYKEGSLAVGATRWQTISKIVVPS
                     AMSGITAAIILGIGRAIGETMAVMMVCGNPTYGLIPQPIVNVFAPIKTITATLGIEMG
                     EVAIGSDHYSALFGLALILLIITLIVNISATFILGRIREKQQSGSKKHPMISFGLKRK
                     IKQFFYLIIGAFLVVILLMAVGIIPTIGILLSIAMMYYILKKISTKIKQRIAFSVISA
                     SLILVLIFLGIILVYIVTNGAGALSWEFITTGPSDLGRSGGIFPAIIGTLYLVAGAIA
                     ISLPLGVSAAIFLQEYTREGTITKIIRAGADLLNGTPSIVFGLFGFAFFVLYLKLGFS
                     LLAGQITLAFMILPTIIRTTEEALKSIPQSLREGSYALGASKWQTIRNVVLPPATPGI
                     LTGAILGIGRAAGETAPILFTAVVFSNFIPGSVLQPVNALPYHLFTLATSIPGSQQNQ
                     AGTALMLLLIVIGIYLIAILLRNRFRKKMKW"
BASE COUNT          696 a          514 c          518 g          696 t
ORIGIN      
        1 tgtgggtgac aatgattacg gtatatttcc ttttcaatcc ggtcataaga tcttcgattt
       61 ttgctgttgc aattggatca agagctgaac atggttcatc catgaggatt acctctggtt
      121 ctattgagag cgatcgtgca atacacaatc gttgttgttg tccaccggaa agacccattg
      181 ctgattcata taatcgatct tttacttcgt cccatagtgc tgcagctttg aggctttttt
      241 ctacgatttc atctagtttc tttttatcgg taattccctg tacttttgaa ccatatgcca
      301 cattttcata tatggttttt ggaaacgggt ttggtttttg gaacaccata ccaatttttt
      361 ttcgtatctc aacagcatcc gcgtctggtt catagatgtt attttcatgg aaataaacct
      421 cccccgtgac tttacatttg tcaatgacat cattcatgcg attgaaacac cgaattaagg
      481 tcgattttcc acagccagaa ggaccaatta acgcggtaat cttatttgga gaaataccaa
      541 gggtgatatc aaataatgct tgtttctcgc cataccaaag atttaggttc tccgttctga
      601 gtatctgttc tttctttccc atgtgatgtt caccatttca ttttttttct aaagcgattg
      661 cgtaggagga ttgcaatcaa ataaatccca atgacgataa gtaacaacat aagagcggtt
      721 cctgcctggt tctgttgtga cccaggtatt gatgttgcaa gggtaaagag atggtagggg
      781 agtgcattaa ctggctgtaa taccgaacct ggaataaaat tcgagaaaac aaccgcggta
      841 aataaaatag gagcggtttc tccagcagcc ctccctatcc caaggattgc tccagtaaga
      901 atccctggtg tcgctggtgg tagtactacg tttcgtatcg tctgccattt tgaagcgcct
      961 agcgcatagc ttccttctcg tagcgattgt ggaatactct ttagtgcttc ttctgtcgtt
     1021 cggataatgg ttggaagaat catgaatgcc aacgtgattt gtccggcaag taaagaaaac
     1081 cctaatttga ggtataggac gaaaaacgca aaaccaaata acccaaagac tatggacggg
     1141 gtcccattga gcaaatcggc acctgcacga attatttttg taattgtacc ttctctagtg
     1201 tattcttgta aaaaaatcgc cgcactaaca cctaatggta acgaaatggc aatagcccca
     1261 gcgactaagt ataatgttcc tatgattgct gggaagatac ctcctgatcg tccaagatca
     1321 cttggtccag ttgtgatgaa ctcccagctc agagctccag cgccattggt cacaatataa
     1381 acaagaataa taccaaggaa gataagaact aggataaggc ttgcagaaat gacactaaat
     1441 gcgattctct gttttatctt tgtcgagatt tttttcaaaa tataatacat catggcaata
     1501 ctaagaagaa taccaatagt cgggatgatc ccaacggcca tgagaaggat tacgacaagg
     1561 aatgccccta tgatgagata gaagaattgt tttatcttcc ttttaagtcc aaaggaaatc
     1621 atcggatgtt tctttgatcc tgattgttgt ttttccctta ttcgccctaa gatgaatgta
     1681 gctgagatat tgacaatcag agtaataatt aagagaatga gagcaagccc gaataacgca
     1741 ctataatgat cgcttccgat ggctacttca cccatctcta ttcctaaggt ggctgtaatg
     1801 gtcttaatag gtgcaaacac gtttacgatt ggctgaggaa tgagaccata ggtggggttc
     1861 ccacagacca tcataaccgc cattgtctcc ccaatggcac gtccaatccc caggatgata
     1921 gcagcggtga tacctgacat cgctgatgga acaacgattt tactaatagt ctgccatcgt
     1981 gtcgcaccaa cagcaaggga tccttctttg tactcacggg gaaccgagct aattgcatct
     2041 tcagcgacac tggtaattgt cgggattgcc ataatgccaa gaagaataga ccccgcaagc
     2101 cacgattctc cgctgggaac gccaaaggtt gtttgaagcc aggaggttag cacaagtagt
     2161 ccaaaaaaac caaacacaac cgatgggata ccagccagaa gctcgatagc tggctttatg
     2221 attgctttta ttcgcggagt tgcaatctca gcgataaaaa tagcgctccc gatgctgagt
     2281 gggatagcga tgaccattgc tccaagtgtg acaagtaagg tgtcgattat aaggggaaaa
     2341 gctccgtaga gtggtggttc tccggtggga taccacctca taccaatgaa aaaattcaca
     2401 acgccttctt tttgaaaaat agga
//
LOCUS       DATMXJ010000091         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_17714, whole genome shotgun sequence.
ACCESSION   DATMXJ010000091 DATMXJ010000000
VERSION     DATMXJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17714"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VN377_02605"
     CDS             complement(<1..484)
                     /locus_tag="VN377_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27309.1"
                     /translation="MVTVISESTAALAISVSIVEGDTFRPGQSFHIITEIRNSKASGR
                     VDVKVTYEVTDLEDNSIVVQSTTVAIETLSSFSEEIQLPKTIKNGVYLLKTNVTSLDG
                     TQYAEVSKSLSVVEVSANQQLFIELLMGGALIITVGGLFYEHKRISKLKVSERDLKKY
                     I"
     gene            complement(724..1704)
                     /locus_tag="VN377_02610"
     CDS             complement(724..1704)
                     /locus_tag="VN377_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HWR27310.1"
                     /translation="MKHVFTRYPLDLLLCVLVSILILPLVFFHVDQTLRVLFGLPFIL
                     FIPGYVLIFVLFPMKKTEKGIDSIERIALSFGLSIAVVPLIGLILNFTPWGIRLEPIL
                     LSLFGFIIIVGLLGWYRWTNTNPAERLTVSLTISIPKAENRIDRLLTIVLGLAILVAI
                     AALMYVIIAPKTGEKFTEFYLLNTERRADDYPTNLTVGEVASVIIGIVNHEYRPITYT
                     VEVWLINQTTVYDNTTQNNETLYNHAWFMDSVTVTLNHTAVDIEAAWEPQFEYNYTFN
                     ISQKGTDLKLVFLLFTTPTDPSLYNRDYKDNIAEKITSAYREIHLFVTVS"
     gene            complement(1714..1956)
                     /locus_tag="VN377_02615"
     CDS             complement(1714..1956)
                     /locus_tag="VN377_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27311.1"
                     /translation="MSEKITLVVAAWILIAFFITGDTDLEVFFILIFIGVLITRQLTD
                     VFTTSNLKDRIDIFIYIFIILFVVIVGKKIINILGM"
     gene            complement(1968..2720)
                     /locus_tag="VN377_02620"
     CDS             complement(1968..2720)
                     /locus_tag="VN377_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27312.1"
                     /translation="MTLPFSLQQPHIRAKIIGILLITFGSIFFLNYFIYNDQLSLKIG
                     FTSLLIGLFTIAMITEKSIPQRISETQIQGNLHAVTTITQALNLSGNAIFLPTSTILT
                     KERVFIPLNNTQRKISESLDDDFVFSTGQGGESLGVALPPSGYSMLQELEKEADFSNT
                     DLDAIEEKLQTFIGMNLLKSISFTQNEKDWRLTVEKPLLCPNNILLCRQYPCPTCSAA
                     LIALTRSSKQKIRINDVIIAGKKTAFHLRPEE"
     gene            2795..>4588
                     /locus_tag="VN377_02625"
     CDS             2795..>4588
                     /locus_tag="VN377_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27313.1"
                     /translation="MWRGFSVVCLCIILLPSTVSAVITPHENFDAATEDLIAIVSFFD
                     DAKMLCEEALRFSLKGNCTIQFGEPLTIQCAQEPLLLSVQKGDELTQKLLYSTDSIGK
                     LQNEAGSYQYLKDFLLPLKELGSNVSLFVTVHRDLTNNLSKVVDGVVNRTNAMYTLYC
                     LVYAQSLVQSLSGTLQDIETNLGAFKEGFSVDTLRGLVADAQQMVILYEGYITTLLTM
                     VESEKPYLSVYVDDTTVYLGEDVSLYGVFLAQRRFIVNQSIDVLWDGQKINMTVTRAA
                     GVFKSSIPVSLTTVPKAHTIMVSTVYNHSAIFSRNVTITVLRIQTRVTLVVPKTHYYL
                     NESILFSGRLVDYKDRGFSAPIILQFAGENVSLRSDGQGNFSYLCTRTLAFGRYGASA
                     AFTPETIYLACVSEKTELSIDTPTVLTFAVAKTNLTIGDALQCTGRLTSMVDGSALEN
                     KTINVFINTNKIGFAMTDTNGYYRFTSGTQALKQNDIYQMYAMFDTTDAQWRSATSEK
                     ITVRIIPIQNAEGIPVLNIAVAIIVALSVSLVLFLLRKKQAPAPTKKGNLTNPTLPIP
                     LIQEVTKTCMDLQEFFADSSTKGKDAFKTAVI"
BASE COUNT         1280 a          892 c         1068 g         1348 t
ORIGIN      
        1 caatatactt cttcaaatca cgttcggaaa ccttcagctt tgaaatcctt ttgtgctcat
       61 aaaacaatcc gccgaccgta ataatcaatg ccccgcccat gagtaactca atgaatagtt
      121 gctgattcgc tgacacctca acaacactta atgatttact cacttccgca tattgcgtcc
      181 catcaagtga ggtaacattt gtcttcaaga gatagacccc attttttatg gtttttggta
      241 attgtatctc ctcagaaaaa gaacttaacg tctctattgc cacggtcgta ctttgaacta
      301 cgatactgtt atcttctaaa tcagttactt cataggtgac cttcacatcg accctgcctg
      361 atgcttttga gtttcttatc tcggtaataa tgtggaacga ttgacctggt cgaaatgtat
      421 caccttctac gatcgaaaca gagattgcca gtgctgctgt agattcggaa atgaccgtta
      481 ccacgcacag gaatgaaaaa agaaaatata cgacaaatag gagtttgtaa atctttccca
      541 agctactttt gaatccttgg tgcaatccca tagccacgct ccaatataca atagtgaaat
      601 aggaagatat tgtttaaatt tttggattga tagttccttt cgtacgaggg tacaaaagag
      661 aatgacaagt ttttccagca gagtgatcat tttcccgtct cgccgatttc tcttctttgt
      721 caattacgaa acggtgacga agagatgtat ttctcggtac gcacttgtga tcttttctgc
      781 gatattgtct ttgtaatccc tattataaag agagggatct gtcggggtgg taaataaaag
      841 gaacacaagt ttgaggtctg ttcctttctg gctgatgttg aaggtatagt tgtattcaaa
      901 ttgtggttcc catgctgctt cgatatcaac cgcggtatga ttgagcgtga ccgtaacgct
      961 gtccatgaac catgcatggt tgtacaaggt ttcgttattt tgtgttgtgt tgtcgtacac
     1021 ggtggtttga ttgatgagcc acacctcgac tgtgtaggtg attggtcggt attcatggtt
     1081 gacgatgccg atgatgaccg atgcaacttc tcccacggta agattcgttg ggtagtcatc
     1141 tgctcgtcgt tcagtgttca gcaggtagaa ctcagtgaat ttctcacctg ttttcggagc
     1201 aataataaca tacatgagcg ctgcaattgc tacaaggatc gctaagccaa ggacgatggt
     1261 aagcaatcgg tcaattctgt tttctgcttt tggtatcgat atcgtcagcg ataccgtgag
     1321 tctctccgca ggattggtgt ttgtccatcg ataccatcca agcagaccta caatgatgat
     1381 aaaaccaaac agcgacagaa gaataggttc tagtcgaatt ccccacggag taaaattgag
     1441 gatgaggccg atgagcggaa ccaccgctat cgacagaccg aaactcagtg cgatacgttc
     1501 gatgctatcg atgcctttct cagttttttt cattggaaac aaaacaaaga taagaacata
     1561 acctgggatg aacaagataa acgggagccc aaaaaggaca cgaagggttt gatcaacatg
     1621 aaagaaaaca agaggcagta tgaggatgct tacgagtaca caaagaagaa gatcgagagg
     1681 ataccgtgta aatacatgtt tcatggtgtc acgttacatt ccaagaatgt ttattatctt
     1741 ttttccgaca atgacaacga agaggatgat gaagatgtag atgaaaatgt ctatccgatc
     1801 cttgagatta ctcgtggtga acacatcagt aagttgacgg gtgataagga caccgatgaa
     1861 gatgagaatg aagaacacct ccaaatcagt gtcaccggtg atgaagaagg cgataagaat
     1921 ccatgctgcc acgacgaggg ttatcttctc cgacattaac acggtgatca ctcctcaggt
     1981 ctgagatgaa acgcggtctt tttccctgca attatcacat cgtttatcct gattttttgt
     2041 tttgatgatc gagtgagtgc aatgagggct gcgctacagg tcgggcaggg gtattgtctg
     2101 cagaggagaa tattattcgg acaaaggaga ggtttttcca ctgtaagacg ccaatctttt
     2161 tcgttctggg taaaagagat ggatttgaga aggttcatgc cgataaaggt ttgtagtttt
     2221 tcttcgatag cgtcaagatc tgtattgcta aaatctgctt ctttttcaag ctcctggagc
     2281 attgaatacc ctgaaggtgg gagtgcgaca ccaaggctct ctcctccctg acctgttgag
     2341 aaaacaaaat catcatcgag agattcagag atctttcttt gggtgttatt gagggggatg
     2401 aagacgcgct cttttgtaag gattgttgag gttggaagaa agatggcatt tcctgaaaga
     2461 ttgagtgctt gagtaatggt ggtaaccgca tgaagatttc cttggatctg tgtttcgcta
     2521 atcctctgag gaatgctttt ttctgtgatc atagcgatgg tgaacagacc aatgagcagt
     2581 gaagtaaacc cgattttgag ggagagttga tcgttataaa tgaagtagtt gaggaaaaaa
     2641 atactcccga atgtaatgag gagaataccg atgatttttg ctcggatatg tggttgttgt
     2701 agtgaaaaag gtagcgtcat aggatatctg tctcaaattg atggtatcga tatcatccaa
     2761 aagaatttaa atgatttcat tgttttcgga gttgatgtgg cggggatttt ctgtcgtctg
     2821 tctgtgtatt attttgttgc catctactgt ttctgcagtg attacaccac atgagaattt
     2881 cgatgccgcc accgaggatc tgatcgcgat tgtttccttt tttgatgatg caaagatgct
     2941 ctgtgaagag gcgttaagat tttcgttgaa agggaattgc acgattcaat ttggtgaacc
     3001 gctcacgata caatgtgcgc aggaaccgct gcttctttcg gttcaaaagg gagatgaact
     3061 cacacaaaaa ctgttatatt ctacagatag tattgggaaa ctacagaacg aggctgggag
     3121 ctatcagtat ctgaaagatt ttttactgcc tttgaaagaa ctcggtagta atgtttcgtt
     3181 gtttgtgact gttcatagag atctgacaaa taatctatcg aaggtagtcg atggtgtggt
     3241 gaatagaaca aatgcaatgt atacgctcta ttgtctcgtt tatgcgcagt cattggttca
     3301 gagtctttca ggcacacttc aagatatcga gacaaatctt ggagcgttta aagaaggttt
     3361 ttctgttgat acgctccgtg gattagttgc agacgcgcaa cagatggtta tcctttatga
     3421 gggatacatt acgacgttgt tgacgatggt ggaaagcgaa aagccatatc tttccgtgta
     3481 tgtcgacgat acgaccgtgt atcttggtga ggatgtttcg ctctatgggg tgtttcttgc
     3541 gcagcgtcgg tttattgtga accagtctat cgatgttctt tgggatggac aaaaaataaa
     3601 catgaccgtg acgcgtgctg cgggtgtttt caaaagttct atacccgtgt cgttgacgac
     3661 cgtgcctaag gcgcatacga tcatggtttc tacagtatat aatcattcag cgattttttc
     3721 aaggaatgtt acaattactg ttctgagaat ccagacccgc gttactcttg ttgttccgaa
     3781 aactcattac tatctgaatg aatcgatact gttttcaggc aggcttgtgg attataagga
     3841 ccgtggtttt tctgcaccta taatcctgca attcgcgggg gaaaacgtct cgcttcgatc
     3901 tgatggtcaa gggaatttca gctacctgtg tacacggacg cttgcattcg gaagatatgg
     3961 tgcatctgca gcttttaccc cggaaactat ctaccttgcg tgtgtgtcgg aaaagaccga
     4021 actcagtatc gatacgccaa cagtcttaac atttgcagta gcgaaaacga atcttactat
     4081 cggagatgcc ctccagtgta caggtaggtt aacaagtatg gtcgacgggt cagcgttgga
     4141 gaataaaacg ataaatgttt tcatcaatac taacaaaata ggtttcgcga tgaccgatac
     4201 gaacgggtat tatcggttca cctctgggac acaggctctg aaacaaaacg acatctacca
     4261 aatgtatgcg atgtttgaca ccaccgatgc gcaatggagg agtgctactt ctgagaaaat
     4321 caccgttagg attattccta tccagaatgc tgagggaatc cccgtcttga atatcgcagt
     4381 cgcgataatc gtcgcactta gcgtatcgct cgttcttttt ttactcagga aaaaacaagc
     4441 acctgctccg acgaaaaaag gcaatctcac caatcctact ctgcctattc ctttgattca
     4501 agaagtaaca aaaacatgca tggacctaca agagtttttt gcagattcct caacgaaggg
     4561 aaaggatgca ttcaagaccg cagtgatc
//
LOCUS       DATMXJ010000092         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7021, whole genome shotgun sequence.
ACCESSION   DATMXJ010000092 DATMXJ010000000
VERSION     DATMXJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7021"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN377_02630"
     CDS             <1..546
                     /locus_tag="VN377_02630"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWR27314.1"
                     /translation="EQFLIKRKELSKSEVEKKSLQIQHRLFSLPWYHTAQNILFYVSY
                     DNEVDTHVMIKESLMNGKTVLVPKTDKEKKTICLSRLLCWEDLSPCAYSILEPRENCF
                     REVPVTSVELLIVPGIVFDVYGNRIGHGMGYYDRLLKNTIQAHSIGLAFEYQIIKAIP
                     AEEHDEKVEMIVTEKRCIHCQ"
     gene            complement(547..1680)
                     /locus_tag="VN377_02635"
     CDS             complement(547..1680)
                     /locus_tag="VN377_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HWR27315.1"
                     /translation="MMLERKIIQTLVESYDPKKITIGVLGGHSALDVCRGAKKHGFRT
                     LAVCQKGRDKTYTKYYKTRDDGRGCIDKTIVLDKFKDITIPAIQEQLRAENTVFIHNR
                     YFWVYFDFTDIENNFRVPIFGTRSMLKLEERNVPKNQYYLLQKAGIRYPQLFQSPHDI
                     DRLVIVKVNEAIRGYERAFFFASSFADYKKKSNQLLAKKMITQDSLEKAVIEEYVIGT
                     QINFNYFYSVLDGELEIMGTDTRRQTNLDGLLRLPADEQLAVLKYLQPKIIETGHIAA
                     TTKESIIESIFELGEKFVETTKKEYPPGIIGPFALQGAIVADKGKEEMVVFDVSLRIP
                     GSPLTQFTPHSGYLYGESISYGERIAIELQKAIQTKRLKEIVT"
     gene            complement(1716..3578)
                     /locus_tag="VN377_02640"
     CDS             complement(1716..3578)
                     /locus_tag="VN377_02640"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWR27316.1"
                     /translation="MKLLLIHSDFIEYEVKEKAIPHPEDTNITKDRLDEALTVFIAVE
                     KVDEKSPSQAVDEASKEIIKTAEQLKVKNIMLYPYAHLSSDLASAKAGKDILIQLEQE
                     VKKQDFNVKRSPFGWYKAFTISCKGHPLSELSREIIPGKEKKAVEKEREMVSQWCILT
                     PEGELVDAEKFNFSGHEELKQLYEYESSGTRKIVGEPPHVKMMQSQELVDYEPASDSG
                     NFRWYPKGRMIKGLLEQHINNMVRDIGGMQVETPIMYDLEHPALSEYVKKFPARQYIV
                     HSDKDFFLRFAACFGQYMIAHDMTISYRNLPLRLYELTHYSFRREQSGEVSGLKRLRA
                     FTMPDLHTFCKDLPQATEEFKRQFSTAMRWMSSLKLDSEVALRFVKEFYENNKTLAQD
                     LAKLAGKPILVELWNERYFYFVMKFEFNVIDSMKKASALSTVQIDVENGKRFNITYAD
                     EKGEKQHPLILHTSISGSIDRNLYALLEREAIKMSQGKKPMLPLWLSPTQVRFIPVAD
                     EFVPDCELFINELNAISPFYYIRSDIDDREESVGRKIRDAEKEWIPYIVVIGEKERKE
                     KTVVPRVRTSELGEGDKPYTVSQLHSLILERVKEFPQQKLPLALYMSKRPKFKG"
BASE COUNT          960 a          812 c          781 g         1161 t
ORIGIN      
        1 gaacaattct taattaaacg aaaagaatta tcgaaatcgg aggtagagaa aaaaagccta
       61 cagattcaac accgcctttt ttctttaccg tggtatcata ccgcccagaa tattcttttt
      121 tatgtttcgt acgacaatga agtcgacact catgtcatga taaaggaatc gttaatgaat
      181 ggaaaaaccg tgttagtccc aaaaactgat aaagagaaaa agacaatctg tttatctaga
      241 ctgctttgct gggaggatct tagtccctgc gcatattcca tcctagaacc gcgtgaaaat
      301 tgcttccgtg aagtcccggt tacttcagtt gagcttctca tcgtccccgg gatcgttttt
      361 gatgtatatg gaaaccgcat cggccatgga atgggctatt acgatcgctt gctgaaaaat
      421 accattcaag cacattctat cggtcttgca tttgaatatc aaatcatcaa agcgatacca
      481 gcagaggaac atgatgaaaa agtagaaatg atagttacgg aaaaacgctg catccattgt
      541 cagtagttaa gtgacgattt ctttgagtcg tttcgtttga atcgcttttt ggagttctat
      601 agcgatccgc tcaccataac ttatggactc accatacaaa taccctgaat gcggtgtgaa
      661 ttgcgttaac ggacttcctg gaattcttaa cgacacatca aagacgacca tttcttcttt
      721 ccctttatca gcgacgatag caccttgcaa ggcgaacgga ccaattatac ctggaggata
      781 ttcttttttg gtcgtttcaa cgaatttttc accaagctca aagatcgatt caatgataga
      841 ttctttcgtc gtcgccgcga tatgacctgt ttcaatgatt tttggttgta ggtactttag
      901 tactgctaat tgttcatcgg caggaagacg aagcagacca tcaagatttg tttgcctgcg
      961 agtatctgtc cccatgatct ccagttcacc atcaaggacc gagtagaaat aattaaaatt
     1021 aatctgggtg ccaatcacat attcttcaat gactgctttt tccaatgaat cctgagtaat
     1081 catttttttt gccagaagct ggtttgattt ctttttatag tctgcgaaac tcgaagcaaa
     1141 gaaaaacgcg cgttcataac cacggattgc ttcatttact tttactatca cgagtcggtc
     1201 gatatcgtgg ggtgattgga acaactgagg ataccggatg ccggccttct gcaacaagta
     1261 atactggttt tttggaacgt tccgttcctc gagcttcagc atcgaacgcg ttccgaaaat
     1321 cgggactctg aaattattct cgatatccgt gaaatcaaag tacacccaga aatatcggtt
     1381 gtgaataaag accgtgttct ccgctcgtaa ctgttcttgg attgctggta tcgtgatgtc
     1441 tttgaattta tcgagaacaa tggtcttatc tatgcagcct cttccgtcat cacgggtttt
     1501 ataatatttt gtgtatgttt tatctctccc tttctgacag acagccagtg ttcgaaaacc
     1561 atgttttttt gctcctctgc agacatcaag ggctgaatgt ccaccgagca caccaatcgt
     1621 gattttttta ggatcatagg attcgacaag ggtttgaatt atcttacgtt ccaacatcat
     1681 acattccatt ccagatagct gaacaaatca tgttcttatc ctttgaattt cggtcgtttt
     1741 gacatataca gagcgagagg taatttttgc tggggaaatt ccttgactcg ctctaagata
     1801 aggctgtgca attgtgagac ggtatacggt ttatcccctt cacctagttc gcttgttcgt
     1861 acacgtggta cgactgtttt ttctttccgt tctttttcac caatcacgac gatataaggg
     1921 atccattctt tttccgcatc acgaatcttc cgcccgacac tttcttcgcg gtcatcgata
     1981 tcagatctga tataatagaa cggtgagata gcattgagct cattaatgaa cagttcgcaa
     2041 tccgggacaa actcatcggc gaccggtatg aatcgaacct gagtgggcga gagccagaga
     2101 ggtaacatcg gttttttccc ctgactcatt ttaatggctt cacgttcgag taacgcatag
     2161 aggttgcggt cgatactgcc tgagatgctc gtatgcagaa ttaatggatg ttgtttttcc
     2221 cctttctcat ctgcgtaggt gatgttgaat cgtttcccgt tctccacatc gatttggact
     2281 gttgaaagcg cacttgcttt cttcatggag tcaatcacat tgaattcgaa tttcataaca
     2341 aagtagaagt accgttcgtt ccagagctca acaaggatcg gcttcccagc taatttggcg
     2401 aggtcctggg cgagtgtctt gttgttctcg taaaactctt tgacgaatcg taaagccacc
     2461 tcggaatcga gcttcaggga actcatccat cgcatcgctg tgctaaattg tcgtttgaat
     2521 tcctctgttg cttggggaag gtctttgcag aaggtatgaa gatcaggcat ggtgaacgct
     2581 cgtaaccgtt tcagaccgct gacttcaccg ctttgttcac ggcggaaact atagtgtgtg
     2641 agctcatata accgcaacgg aaggttccgg taggaaatcg tcatatcatg ggcgatcata
     2701 tactgcccga agcacgctgc aaagcgtaga aagaaatcct tatcagaatg aactatgtat
     2761 tgtcttgcag ggaatttctt gacatattca cttaaggcag ggtgctcaag atcgtacatg
     2821 ataggtgtct ctacttgcat gccaccgatg tctcgcacca tgttattgat atgttgctcc
     2881 aacagacctt ttatcatcct tcctttggga taccaacgga aattccctga gtcgctggca
     2941 ggttcataat ccactagctc ctgtgattgc atcattttca catggggcgg ttcaccgacg
     3001 atctttcgtg tgccactgct ttcgtattca tacaattgtt tcagttcctc atgacctgag
     3061 aaattgaatt tctccgcatc cacaagttca ccttcagggg taagaatgca ccattgagag
     3121 accatctcac gttctttttc cactgccttc ttttctttcc ccgggatgat ttcacgggag
     3181 agctctgaaa gaggatggcc tttgcaggag atggtgaaag ccttgtacca accaaatggt
     3241 gatcgtttca cgttgaagtc ctgttttttt acttcctgct caagttgtat gaggatatcc
     3301 tttccagctt tagcactcgc aagatctgat gaaagatgtg cataagggta taacatgatg
     3361 tttttgactt tgagttgttc cgcggtcttt ataatttctt tgctcgcctc gtcgacagcc
     3421 tgcgatgggg atttttcatc tactttctca acagcgataa aaacagtgag agcttcgtct
     3481 aagcgatcct ttgttatgtt cgtgtcctca ggatgcggta tcgctttttc tttgacctca
     3541 tattcgataa aatcactatg gataagtaat agtttcatag aatcgacccg taaagcaaat
     3601 cagtgttttt aaagatagtg attctcgtta ttatatgggg gaggcggcgg aggtgtatac
     3661 tccgtaacta aaaaattttt tgttttactt gcaatcccat ctctccgcct aacc
//
LOCUS       DATMXJ010000093         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12442, whole genome shotgun sequence.
ACCESSION   DATMXJ010000093 DATMXJ010000000
VERSION     DATMXJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12442"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN377_02645"
     CDS             <1..445
                     /locus_tag="VN377_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWR27317.1"
                     /translation="YKNGYFSIENIRDAPIDDLVQIRGIKRKLAKQIKTEIEDQITKT
                     DTSEFIPAKHRTTKKKEKRKLQDSAEWESSASKVKTQKSSSFDVCTYKEYTLYQRETK
                     TPDGKKSTLHYFTKSKSDKGHPSTLPKGYRIALNKKTGVPYLKKR"
     gene            505..1974
                     /locus_tag="VN377_02650"
     CDS             505..1974
                     /locus_tag="VN377_02650"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWR27318.1"
                     /translation="MNWKGPLRKINDFRFEIPSNYQGAQNNLHMKTSGIIYANNGMIE
                     CIRTDNAPEQVANVTMLPGIVGKSLAMPDIHWGYGFPIGGVAAMDEETGVISPGGVGY
                     DINCGVRLLRTNLQFTDLDPQKIQALIDEMFINVPSGLGSKAKVHLSRKQLDDVLKLG
                     AKWAIENGYGWNEDAEYIEEHGCLEYADISKTSNEAKQRGAPQLGSLGAGNHFLEIQK
                     VAEIYDPIAAKTFGIQNIGQIMVMIHTGSRGFGHQVCTDHLRVLEQAVKKYNIWLPDR
                     QLACAPTTSKEGQDYLKAMACAANFAWCNRQMIVHWVRESFEKILHQRADDMDMGIVY
                     DVCHNIAKLEEHEIDGKKRKVYVHRKGATRSFGPGKQEVPLKYRDIGQPVLIPGDMGT
                     ESYLLHGTKNSEETFGSTCHGAGRVMSRTEAVHRYRGEQIMSELRKKGIYAHPASWKV
                     MAEEAPDAYKDVSEVVNVTHGAGISLKVAKLLPLGVVKG"
     gene            2042..2806
                     /locus_tag="VN377_02655"
     CDS             2042..2806
                     /locus_tag="VN377_02655"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWR27319.1"
                     /translation="MPLEDIEIVDFEEMSLTNGTVIAGFPSIGLVSTIVANYMIDALG
                     LKQIGAMESPHFPTLSVVHTGEPLSPVRIYGGTLANGQKLAVFVSEFKPKPNLINSLS
                     NAIMIWSKKKGCKLMISPEGMVVEGKSTEDELIVDAYAIGSTENARATLAAKNIPQFK
                     NGIIAGVSGVLLNKGKHEKFDVISILAEAHPNYPDARAAASAINVIALVIGIDINVGP
                     LYDEAERIEKQLQRFHKQAKPIVSGASQIPQPEMYG"
     gene            2887..3636
                     /locus_tag="VN377_02660"
     CDS             2887..3636
                     /locus_tag="VN377_02660"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HWR27320.1"
                     /translation="MHKIPKSHPRYHSLMTREQISEAMKQGIVHETGLIAHGRGEAFD
                     YLLGEMTIPQADAAEKVAAATLLCAKNPVISMNGNVTALVADECISLAKSIPATLEVN
                     LFHRSPQRVKKIAALLRKKGVETLHGVHPDAKIPGLDSDRALCEKIGIFTADVVLVPL
                     EDGDRCQALQKMGKTVIAIDLNPLSRTAQTATITIVDNVIRAIPQIERWVCTLKETEH
                     SNLEKLIRAWDNSKNLEKIMGFLSKRLNSLS"
BASE COUNT         1166 a          732 c          822 g          971 t
ORIGIN      
        1 ctataaaaat ggatattttt ccatcgaaaa cataagagat gctcccatag atgatcttgt
       61 tcaaatccgt ggaatcaaac gaaagctcgc aaaacaaatt aaaacagaaa tcgaagatca
      121 aatcacaaaa acagatacat ctgagtttat acctgcaaaa cacagaacaa ccaagaaaaa
      181 agaaaagaga aaacttcagg attcagcaga atgggaatca tccgcttcaa aggtgaaaac
      241 acaaaaatca tcctcctttg acgtctgtac gtataaggag tacacccttt atcaaagaga
      301 aaccaagaca cctgatggaa agaagtcaac acttcattat tttactaaaa gtaaatccga
      361 caaaggacac ccttctaccc ttccaaaagg gtatcgcatc gctctcaata aaaaaactgg
      421 ggtcccatac ctaaaaaaaa gataacagta cacgtctcat agcttttata aatccttctt
      481 tctattccca agtagagaag ttctatgaat tggaaaggcc ctttacggaa aataaatgat
      541 tttcgttttg aaattccttc aaattatcaa ggcgcacaaa acaatcttca tatgaaaaca
      601 tctgggatta tttatgcgaa caatggaatg atcgaatgca ttcgtacgga caacgctcca
      661 gagcaagtcg caaatgtcac catgcttcca gggattgtcg ggaaatcact tgcgatgcct
      721 gatattcact ggggatatgg gtttcccatc ggtggtgtgg ctgcaatgga cgaagaaacc
      781 ggggtgattt caccgggagg tgttggatat gatatcaact gcggtgtccg attactacgc
      841 acaaacctcc aattcactga tttagatcca caaaagatac aagctcttat tgatgaaatg
      901 ttcatcaatg tcccctccgg tcttggctca aaagcaaagg ttcacctcag tagaaaacaa
      961 cttgatgatg tgttaaagct gggggcgaaa tgggctatcg agaatgggta tgggtggaac
     1021 gaagatgcag aatatatcga agaacatggt tgtttagaat atgcagatat ctcgaaaaca
     1081 tcaaatgaag caaaacaacg cggggctcct cagcttggtt cccttggagc agggaatcat
     1141 tttttagaaa tacagaaagt cgcagagatt tatgatccga ttgctgcaaa aacatttggg
     1201 attcagaata tcggtcaaat tatggtcatg attcatactg gttctcgtgg gtttggtcat
     1261 caggtctgta cggatcatct ccgagtgttg gaacaggcgg taaagaaata taatatatgg
     1321 cttcctgacc gacagctcgc ctgtgcacca acgacctcga aagaaggcca agactatttg
     1381 aaagcaatgg catgtgcagc gaacttcgcc tggtgcaata gacagatgat tgtccattgg
     1441 gttagagaat cctttgaaaa aatccttcat caacgtgccg atgatatgga catgggtatt
     1501 gtctatgatg tctgccataa catcgcaaaa cttgaggaac atgaaatcga tgggaagaaa
     1561 cgaaaagtat acgttcatcg caaaggtgcg acgcgttcgt tcggacccgg aaaacaagag
     1621 gttcccctta aatatcgtga tatcggtcaa cctgttttga ttcctggtga tatgggtaca
     1681 gaaagctatt tgcttcatgg tacaaagaac tctgaagaaa catttggttc tacctgtcat
     1741 ggtgcaggcc gagtcatgtc aagaactgag gcagtgcatc gctatcgtgg ggaacaaatc
     1801 atgagcgaac tgagaaagaa aggtatttac gctcaccctg cgagttggaa agtgatggct
     1861 gaggaagcac ctgatgcata caaggatgtc agtgaagtcg tcaatgtcac ccatggtgca
     1921 gggatttctc taaaagttgc aaagctctta ccgttaggag tggtgaaagg ataattgcta
     1981 gtgttttcct accaattagt tttaaatgct tgggagatat tacagtgcaa gataggtggt
     2041 gatgccgttg gaagacatcg aaatcgtaga ttttgaagag atgagtttaa caaacggaac
     2101 tgttattgca ggatttccat ccataggcct agtaagtacg attgttgcca attatatgat
     2161 cgatgcactt ggtctaaaac agattggagc gatggaatca ccacattttc ccactctttc
     2221 tgttgttcat actggagagc cactatcccc tgtacgaatc tatggtggga cattagcaaa
     2281 tgggcagaaa ctggcagtct ttgtttctga atttaaacca aaaccgaatt taattaactc
     2341 attatcaaac gcgattatga tctggtcgaa gaaaaaaggt tgtaagttga tgatttcccc
     2401 tgaaggtatg gtcgttgaag gaaaatcaac tgaggatgag ctgattgtgg atgcgtacgc
     2461 gatcgggtca acagagaatg ctcgggcaac cctagcagcg aaaaacatcc ctcagttcaa
     2521 gaacggaatc atcgctggtg tctcgggtgt attgttaaat aaaggaaagc atgagaagtt
     2581 cgatgtgatc tccattcttg cagaagcaca tccgaattat cctgatgccc gggctgctgc
     2641 gtctgcgatt aatgtcatcg cccttgttat tgggattgac ataaacgtcg gcccattata
     2701 tgacgaagca gaacggattg aaaaacagct tcagagattc cacaaacaag caaaaccgat
     2761 tgtctctggt gctagtcaga ttccacaacc agaaatgtat ggttaaagcg atttttttcc
     2821 tccttttatt ttctatattt tctccctaag ttttaaaaag gttcttgagt tattcaagct
     2881 gtccctatgc acaagattcc aaagagtcat ccacggtatc attctttaat gacacgagaa
     2941 cagatttctg aggcgatgaa acagggtatt gttcatgaga ccggattaat cgcgcatggt
     3001 cggggagaag catttgatta tctccttggt gaaatgacta tcccgcaggc tgatgctgca
     3061 gaaaaagtag cggccgctac cttgttatgt gcaaaaaacc ctgtgatctc catgaatggg
     3121 aatgtcactg cattggtcgc tgatgaatgc atttctcttg cgaaaagtat tccagcaaca
     3181 ctcgaggtga acctttttca tcgaagccca cagcgagtca agaaaatagc tgctttgctt
     3241 cgaaaaaaag gcgtagagac actccatggt gttcatccag atgccaaaat ccctggtttg
     3301 gattcagatc gtgcattgtg tgaaaaaatt ggaattttta ctgccgatgt cgtcctcgtt
     3361 cccctcgaag acggtgaccg ctgccaggcg cttcagaaaa tgggaaaaac cgttatcgct
     3421 atcgatttga atcctctgtc acggactgcg cagaccgcta cgatcactat cgttgataat
     3481 gtgattcggg caataccaca aatagagcgg tgggtgtgta cattaaagga aaccgaacat
     3541 tctaatcttg aaaaacttat cagagcatgg gataattcaa aaaatcttga aaaaatcatg
     3601 ggtttccttt caaaaagact aaattccttg tcttaataca gggtgtcata tgtatcgaag
     3661 tcattactca aaagaaatct cacaaaaaga g
//
LOCUS       DATMXJ010000094         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_38736, whole genome shotgun sequence.
ACCESSION   DATMXJ010000094 DATMXJ010000000
VERSION     DATMXJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38736"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VN377_02665"
     CDS             complement(<1..192)
                     /locus_tag="VN377_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HWR27321.1"
                     /translation="MKIGGFQKTSLLDYPDRISAIVWTSGCNFRCPFCYNKTLALGTA
                     EMFPQDEILSFLSKRKALLE"
     gene            complement(189..>3443)
                     /locus_tag="VN377_02670"
     CDS             complement(189..>3443)
                     /locus_tag="VN377_02670"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HWR27322.1"
                     /translation="ELKRSEIPTVEQVQDAVEKVIIEDGHVKTAKAFILYRKQHQDMR
                     EIGGLLKDIDVVDDYLSMLDWKVNENSNMSYSLQGLNVYATESIISHYWLNKIYPPEM
                     GEAHTRGDYHIHDLGTLGAYTYFGKENVLIKTKEKILLTSFQKLYEDLEEEEILLSKD
                     DDAWSKYPTGLQVLDVNGWTSLQRVVRKAKRKPMRFLKNESGRSVIVTEDHPMVMGTE
                     IFKKAKDVKEEDDKLYTADLKGLLKNESLFFKDHLFIAQELYDKGLRDFWVEGMPFDE
                     YQTYSQDLQIEGIVSTSNNADALPNKILLTKNLGYFVGFYLAEGWMEPHRICMKQKNT
                     SENQEIIHRVIQAAHELGCRIYSRDEDGFVTLRIANSTLVEYLFKFIFNAEGISKEKC
                     LPVEILTYNLQFVKGLIAGILDGDGSLATSKTNLVIRTASRTMLEQLAMVLNILGFTA
                     RDRNIEGVGTTREYHGRQIIQQYPLFGVSFRTTSATLPSQLYQSAQISSKAWRAEGFG
                     WHKVLNNEPTEILDDYIYDITTSSHTLIVNGMWNHNCVGWDLKDLLLLGFKGVSGKIE
                     SKPAKHFSTALMQIVNYLYTLQGEAAGAQALSNFDTLLAPFIEKDGLQYENVKQEMQK
                     FMFNMNVPTRVGFQSPFTNITMDLTVPGYMSEEPAIIGGELTDNNYNEYSDEMDMMNH
                     AFAEIMNGGDAKGRPFTFPIPTYNITKDFNWENDGLEPLWEMTAKYGIPYFSNFINSD
                     MKPDDARSMCCRLKLDNRELRKRGGGLFGANPKTGSIGVVTLNMPRIGYLAKDDNDFF
                     ERLDRLMVLAKQSLEIKRELIENLTASGLHPYSRFYLSDVNKVFGEYWKNHFSTIGLI
                     GMNDALLNFMNKSIADKEGKAFAEKVLDHMRNRIANFQEETGNIFNLEATPGEGTSYR
                     LARIDKKEYPEIRTFNQEVYTQNRGRDVEPYYTNSTQLPVGFTTDVFEALDLQDSLQT
                     KYTGGTVLHIFLGEEEPSPTATKKLVRKVAENYALPYYTITPTFSVCPDHGYIAGEHQ
                     LCPICQAAQKTTECEIYSRVVGYIRPVNQWNKGKQQEFRDRTTFDLREKVLVTA"
BASE COUNT          812 a          808 c          759 g         1064 t
ORIGIN      
        1 ttcgaggagt gcttttctct tcgagagaaa tgagaggatc tcatcctgag gaaacatctc
       61 tgcagtcccc agtgcaagag tcttgttata acagaaggga cagcggaaat tgcatccgct
      121 ggtccagacg atcgcgctga tccgatcagg ataatcaagc aacgatgttt tctgaaagcc
      181 acctatcttc atgctgtgac aagaaccttc tcccggaggt caaaggtggt ccgatcgcgg
      241 aactcctgtt gtttcccctt gttccattgg ttcaccggcc ggatataccc aacgacacgg
      301 gagtagattt cacattccgt ggtcttttgt gcagcctgac agattgggca gagctgatgc
      361 tcgccagcga tgtacccatg gtccgggcag acgctgaacg tcggggtgat cgtataatac
      421 gggagcgcgt agttctcagc aacctttcgg acaagtttct tcgtcgcggt tggtgatggt
      481 tcctcctcac caaggaagat atgcagtact gtgccgccag tgtatttggt ctgcagggaa
      541 tcctgcaagt ccagggcttc gaacacatcg gtggtgaagc caaccggtag ctgcgtggag
      601 ttcgtgtagt aaggttcgac gtccctgcct cgattctggg tatagacctc ttggttgaag
      661 gtccgtatct caggatattc ttttttgtcg atccgcgcca acctgtagct agttccttcc
      721 ccaggtgtcg cttcaaggtt gaagatattt cctgtttctt cttggaaatt cgctattctg
      781 ttacgcatat gatccaggac tttttccgca aacgccttgc cttctttatc tgcgatgctt
      841 ttattcatga aattcagtaa cgcatcgttc atacctatca gaccaatggt agagaaatga
      901 ttcttccagt actcaccaaa gaccttattc acatcagaaa gatagaatcg tgagtatgga
      961 tgcagtccgc tcgcagttag attttcaata agctctcgtt tgatttccaa actctgtttc
     1021 gcaagcacca taagcctgtc gagtcgttcg aaaaaatcat tatcatcttt tgctaggtaa
     1081 ccaattcgcg gcatattcag ggtgacaaca ccgatagaac ctgtttttgg gttcgcaccg
     1141 aagagccctc cgccccgttt ccttaattcc ctgttgtcca atttaagtct acaacacata
     1201 ctgcgtgcgt cgtctggttt catgtcacta ttgatgaagt tgctgaagta tggtatcccg
     1261 tatttcgcgg tcatctccca gagtggttca aggccgtcgt tctcccagtt gaaatccttc
     1321 gtaatgttat acgtcggtat tgggaatgtg aatggtctgc ctttggcatc gccgccgttc
     1381 atgatctctg cgaacgcatg gttcatcatg tccatctcat cagagtactc gttgtagtta
     1441 ttgtcggtga gctcccctcc gataatcgct ggctcctcac tcatgtaacc agggacggta
     1501 aggtccatag taatgttcgt gaacgggctt tgaaacccga ccctcgtagg cacattcatg
     1561 ttgaacatga atttctgcat ctcttgtttc acgttctcat actggagacc gtctttttct
     1621 atgaacggtg caagcaaggt atcaaaattc gataatgcct gtgcacccgc agcctcaccc
     1681 tgtagcgtgt atagataatt cacaatttgc atcaacgcag tactgaagtg ctttgctggt
     1741 ttactttcga ttttcccaga gactccttta aacccgagta acaataaatc cttgaggtcc
     1801 caaccgacac agttgtgatt ccacataccg tttacgatga gggtatgtga tgatgtcgtg
     1861 atatcgtaaa tgtagtcatc aaggatttcc gttggttcat tgttcagaac cttatgccat
     1921 ccgaatcctt ccgctcgcca tgctttggac gagatctgtg ccgactgata caactgggag
     1981 gggagcgtgg cactcgtcgt tctaaaactt acaccaaaga gtggatattg ctggatgatt
     2041 tgcctaccat ggtattccct tgtcgtacca acaccctcga tatttctatc cctcgcggta
     2101 aatcctaaga tattgagaac cattgccaat tgctctaaca ttgttcttga tgccgttcgt
     2161 atcacgaggt ttgtttttga ggtggctaga gacccgtcgc catcaagaat tcctgcgatg
     2221 agtcctttca caaactggag gttgtatgtc agaatctcaa ccggcaagca tttttcttta
     2281 ctgattcctt cggcgttgaa aataaattta aaaagatatt caactaaggt ggagttggcg
     2341 attcttagcg tcacaaatcc atcttcatct cttgaataaa ttctgcaacc taattcatgc
     2401 gctgcttgta ttacacgatg aatgatctct tgattttcgc tggtgttttt ttgcttcatg
     2461 cagatacggt gaggctccat ccatccttca gcaagataaa atccgacgaa ataaccaagg
     2521 ttctttgtta acagtatctt gttcggtaat gcatctgcat tattggatgt tgagacaata
     2581 ccttcgatct gtaaatcttg tgagtaggtc tggtactcat cgaacggcat tccttcaacc
     2641 cagaaatcac gtaagccctt gtcgtataat tcttgtgcga tgaagagatg atccttgaaa
     2701 aagagtgatt cgtttttgag taatcctttg aggtcagcgg tgtataattt atcgtcttct
     2761 tctttgacgt ccttggcttt tttgaagatt tcggtgccca tgaccatggg gtgatcttct
     2821 gtgacgatga ccgatcgtcc gctttcattc ttgaggaatc gcatgggttt ccgtttcgct
     2881 tttctcacaa cccgctggag agaagtccat ccattgacat caaggacttg caatccagta
     2941 ggatattttg accaagcgtc atcgtcttta gataataata tttcttcttc ctcgaggtct
     3001 tcatataatt tttgaaaaga agtaaggaga attttttctt ttgttttaat gaggacgttt
     3061 tccttgccaa agtaggtata tgctcccaag gtccccaaat catgaatatg gtaatctcct
     3121 ctggtgtgcg cctctcccat ctcaggagga taaatcttat tgagccagta atgagaaata
     3181 atgctctcgg tcgcgtacac attaagtcct tggagcgagt agctcatgtt gctgttctca
     3241 ttgaccttcc agtccaacat gctgaggtag tcatcaacca catcaatgtc tttcagaagc
     3301 ccaccgattt cacgcatgtc ctggtgttgt ttccggtaga ggatgaatgc tttcgcagtt
     3361 ttcacatgac catcctctat gatgactttt tctacggcat cctggacttg ttcaactgta
     3421 gggatttcac tgcgtttcaa ctc
//
LOCUS       DATMXJ010000095         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33317, whole genome shotgun sequence.
ACCESSION   DATMXJ010000095 DATMXJ010000000
VERSION     DATMXJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33317"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VN377_02675"
     CDS             complement(<1..539)
                     /locus_tag="VN377_02675"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HWR27323.1"
                     /translation="MNQILAIIPARSGSKRIPKKNIKMLYGKPLLAYTIDSVKNSCHV
                     TRFVVSTEDNEIAKISQSYGAEVIIRPPELARDESPTEDVIINVLEQLKNKEQYSPDI
                     IILLQPTSPLRTTDDIDGAIKTFLASSGDSLISVTEYGHTPYWAFRIEKGFLKQEFTL
                     GPLKRSQDLPTLYRPNGAI"
     gene            complement(636..2189)
                     /locus_tag="VN377_02680"
     CDS             complement(636..2189)
                     /locus_tag="VN377_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27324.1"
                     /translation="MTSSIEKTTPEQLASQIINFYNEYDLKVSGKINNKTAYQLIETI
                     EQKYKTDDIRLSDGTRIWNLLRIFLYSNFQKIDTKTTQRKLSKNNLKSVLSVFKESFQ
                     PLHLPKNVTVCGFSSSESRKLYNNTYYDIYLDPLYEILGDKLAVFEWPETTGYRRTYD
                     HPVFSKHYVPMHFPLWSKTFWDLLANRLTSRRNFTLESEDVLKEIIGFISTTASVDKC
                     VLTKDINDFISVFVSIKKFLYDILETMKPKAVLIRCGYGRFPMALSQACRELNIPPIE
                     IQHGMITAFLPAYRRTTPTTNTDCIPEYLLTQGEIYTKLVQDGNLFEKNKVISVGYPY
                     LHQTLDAGNRPSGKKQTVSPFPQNILFTSQWILASEIQDFILKVAEQFTRDKMDIGIL
                     FKPHPYDNNDYSDMKTNDPIILIGKYEDTYKLFPSADIHSTVYSTSGLEAMAFGIPNI
                     FVDLFNMTHNNTTPYIVASPTQFTQSVRTILKQYDDSVSETKAAADLFFTPSPEKHFK
                     KFFAELGII"
     gene            2250..3245
                     /locus_tag="VN377_02685"
     CDS             2250..3245
                     /locus_tag="VN377_02685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR27325.1"
                     /translation="MAEDFPKVSFLIPLLNEEKTLSQCLDSLLVLEYPSEKMEILLAI
                     GKCTDNTRGIAQEYAKKYSHIRILENPTGNTSIGRNICIHHATGELLMNYSGHVIAEK
                     NLLKDLAVKLVSLPADVAAVGCSNLSPGKQNFVGEVSGAAFLSFMGGRNFFSQNAVFP
                     EERYTDHLSFSCYRKEAVEKVGGFDPVFWCGQDYELDIRLRKAGYKILYTPRTKVYHF
                     KRDSVRSLWRQMYRYGIARAKMVKKHPDTLRFFYLLGPGFILGGVLIVILTVVGVLPV
                     WVVPCLVVAYILMSMVSTLQITRKPSIVVSSVLFYLMIHVGYGAGFLRGIMYSKL"
     gene            3333..4346
                     /locus_tag="VN377_02690"
     CDS             3333..4346
                     /locus_tag="VN377_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR27326.1"
                     /translation="MRKLNIAVIGVGHIGSQHAKHANHLGTLVAVCDIKQDRAKTVAE
                     QYRCKAYFSLDELLKNEKNLDLIAICTPNGLHAEHSIKALKAGCNVLCEKPMATTVRD
                     CERMIHSAEETNRRLFIVKQNRFNPPVKAVKKVIDEGKLGKILSVQVNCFWNRNREYY
                     LGSDWRGKLAMDGGTLFTQYSHFIDLLYWFVGDVEEVRAFTENYLHEGVVEFEDTGVV
                     VLRFTNGALGTIHFTINSFGKNMEGSITLFGEKGTVKVGGQYLNLLEYQSIDGYEIMG
                     LEPGNPPNVYGKNTGSMSNHEKVYENVIDVLVHGGSITTDLLDGLKTVQIIEKIYKAA
                     MKR"
     gene            4366..4833
                     /locus_tag="VN377_02695"
     CDS             4366..4833
                     /locus_tag="VN377_02695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWR27327.1"
                     /translation="MRNRPTIKKVQIKDVTFGENVTVVEPVNLYECEIGDDCFIGPFV
                     EIQRNVKIGKNCRVQSHAFICELVSIGDFCFISHGVKFVNDLFSLGKPAGGDRSLWRS
                     TKVGSYVSIGTNATILPVIITDNVVIGAGSVVTKDIKESGVYAGNPARRLRSL"
     gene            4846..>5455
                     /locus_tag="VN377_02700"
     CDS             4846..>5455
                     /locus_tag="VN377_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HWR27328.1"
                     /translation="MNIPLVDLKVNYHSIKKEIDSAIKDVLESSSFIMGPYVKKFEED
                     FARFCQVKHGIGCANGTAALHLALLAAGIKKGDEVLTVPNTFIATTECISYVGGKIKF
                     VDASPQTALLDIDALEKAITRKTKALIVVDLYGQMPDMQRIREIAVKNDLFVIEDAAQ
                     AHAAEWNGHQPGFYGDIACFSFFPAKNLGCYGDGGAVMTNYDE"
BASE COUNT         1422 a          944 c         1445 g         1644 t
ORIGIN      
        1 aaaatcgcac catttggtcg gtagagagta ggtaagtctt gagaacgctt cagtgggcct
       61 agggtaaatt cttgttttaa gaatcctttt tcgatcctaa aagcccaata cggtgtatga
      121 ccatattctg tgacactaat aagagaatct ccagacgagg ctagaaatgt cttgattgcg
      181 ccatcgatat catctgttgt acgtaagggg gatgttggtt gcaacaaaat gattatatct
      241 ggagaatatt gttctttatt ttttagttgt tcgaggacgt tgatgattac atcctctgtc
      301 ggactttcat ctcttgcgag ttcaggtggt cttatgataa cttcagcacc gtaagattgt
      361 gaaatttttg cgatttcatt atcttcagtc gatactacga aacgagtgac atggcatgaa
      421 tttttcacag aatcaatcgt atacgcaaga agtggttttc catacagcat ctttatattt
      481 tttttcggga ttcgtttgct cccagacctg gcgggtatta ttgcaagaat ttggttcatg
      541 atctcaagct caaagaggat atgtgtttta tatgcattgt ttatttttga gtaacatatt
      601 catttcaata aaataacttt ttccctccag tccttttaaa tgatacctag ctcagcgaag
      661 aactttttaa aatgtttttc aggagaaggg gtgaagaaaa ggtctgcggc agcttttgtt
      721 tcagataccg aatcgtcata ttgtttgagg atggtccgta cagattgggt aaactgtgtt
      781 ggggatgcaa caatgtacgg tgttgtattg ttatgcgtca tgttgaagag atcaacgaag
      841 atgtttggta tcccgaaggc catcgcttca agtccactgg tggaatagac ggttgagtgg
      901 atatctgcag atggaaaaag cttgtaggtg tcttcgtatt taccaattag aatgataggg
      961 tcattggttt tcatatcaga atagtcgttg ttatcatagg gatgtggttt gaaaagaatc
     1021 ccgatatcca ttttgtcgcg tgtaaactgt tctgcaactt tgagaatgaa atcttgtatt
     1081 tctgaggcaa gaatccactg ggatgtgaaa agaatatttt gcggaaatgg ggatactgtt
     1141 tgtttcttac cggatggcct gtttcccgcg tccagggttt gatgaagata gggatatccc
     1201 acggagataa ccttgttttt ctcgaagagg ttaccatctt gtaccagttt tgtgtagatt
     1261 tcaccttggg tgagaagata ttcgggaatg caatcggtgt tcgtcgtggg cgtcgtccgt
     1321 cggtatgcag ggagaaacgc agtaatcatg ccatgctgaa tctcaatagg cgggatgtta
     1381 agctcacggc aggcctgaga gagcgccatg ggaaatctcc cgtacccgca tcgtatcagc
     1441 acggcttttg gtttcattgt ttcaagaata tcatagagaa attttttgat agaaacgaag
     1501 acagagataa aatcatttat atctttagta agcacgcatt tatcaactga ggcagttgtg
     1561 ctaataaatc cgatgatttc ttttaataca tcctctgatt ctaatgtaaa gtttcttcga
     1621 ctcgtcagtc tattggcaag aagatcccag aaggtctttg accagagggg aaagtgcatg
     1681 gggacataat gttttgagaa cacaggatga tcatatgttc ttcggtaccc ggtggtttct
     1741 ggccattcga aaaccgcgag tttgtctcct aatatctcat acagaggatc aaggtagatg
     1801 tcataatagg tgttgttgta gagttttctg ctttcgctac tggagaatcc acaaacagtg
     1861 acgtttttag ggagatgaag cggctggaag ctttctttaa aaacagaaag aactgattta
     1921 agattgtttt tgctcagttt tctctgagtc gtcttggtgt cgattttttg gaaatttgaa
     1981 tataggaaga tccgtaggag gttccagatt cgtgtcccat cagagagtcg tatgtcatct
     2041 gttttatatt tttgttcgat ggtttcaatg agttggtatg ctgttttatt gttgattttt
     2101 ccagagactt ttaggtcata ttcattgtag aaattgatga tctgtgaagc aagctgttct
     2161 ggtgtagttt tttctattga cgaggtcatt ttcttatcaa ctgagtagtg tgattaatat
     2221 taaatacgtt ctgttgtttt ccagaagaga tggcagagga tttcccgaag gtttcatttc
     2281 tgataccgct tttaaatgag gaaaaaacac tttcacagtg cctagattct ttacttgtat
     2341 tggagtatcc ctctgaaaaa atggagatct tgcttgcgat cgggaaatgt accgataaca
     2401 cgaggggaat tgctcaggaa tatgcaaaaa aatattccca tatccgaata cttgaaaacc
     2461 cgacagggaa cacctcgatc ggtcggaaca tatgcattca ccatgcgact ggtgagctgc
     2521 tcatgaatta ttctggtcat gtgattgctg aaaaaaactt attaaaagat cttgcagtaa
     2581 aattggtaag cctccctgct gatgtcgccg cggttggttg ttcgaatctt tccccgggga
     2641 aacagaattt tgtgggggaa gtatctggtg ctgcgttttt gagttttatg ggtggtagga
     2701 atttcttttc ccagaacgcg gtgttcccgg aggagcgata cacggatcac ttgtcgttct
     2761 cctgttatcg aaaggaggcg gtggaaaagg tcggcgggtt tgatcctgtg ttttggtgtg
     2821 gtcaggatta tgagcttgat atcaggcttc ggaaggcagg gtataaaatc ttgtacacac
     2881 cgaggacgaa ggtatatcat tttaagcggg attctgttcg atcgttatgg cgtcagatgt
     2941 atcgatatgg aatcgcacgg gcgaagatgg tgaaaaagca tcctgatact ttgaggtttt
     3001 tctatttgct tggaccgggg tttatccttg gtggagtcct catcgtgata ctgacagtgg
     3061 ttggtgtatt gcccgtatgg gtggtcccct gtctggtcgt ggcatatatc ctgatgtcga
     3121 tggtcagcac cttgcagata acgcgaaaac ccagcattgt cgtaagtagt gtgttgtttt
     3181 atttaatgat ccatgtggga tatggtgctg gttttctacg aggaatcatg tacagtaaac
     3241 tataggaaac atgcctgtta ttcttaaata ggggggagga aatatcggta gtgactccac
     3301 gtttttttct cgtgggaaaa gagagacggg atatgagaaa gttaaacatc gcggtcatag
     3361 gtgtcggtca tattggctcc caacatgcga aacatgcgaa tcacctgggg accttggtcg
     3421 cagtgtgtga tattaaacaa gaccgggcga agactgtcgc tgaacaatat cgctgtaagg
     3481 cgtatttttc ccttgatgaa ttgctcaaaa atgagaaaaa tctcgattta atcgctattt
     3541 gtactccgaa tggcctgcat gcggaacata gcatcaaagc gttgaaggct ggttgtaacg
     3601 tgctttgtga aaaaccgatg gctacgacgg tgcgggattg cgaacggatg attcattccg
     3661 ccgaggagac gaaccgacgg ctgttcatcg taaaacaaaa ccgattcaac cctccagtaa
     3721 aagctgtaaa aaaggttatt gatgaaggga aacttgggaa gatcctcagt gtgcaggtga
     3781 attgtttctg gaatcgaaac cgtgagtact atctcgggtc cgattggcga ggaaagctag
     3841 cgatggatgg cgggacgctg ttcacccaat acagccattt cattgatctt ttatactggt
     3901 ttgttggtga tgttgaggag gtccgggcat tcaccgagaa ttatctccat gagggagtcg
     3961 tggagtttga agacaccggt gttgtggtgc tacggtttac gaacggtgca ctagggacga
     4021 ttcattttac gataaattcc tttgggaaaa acatggaagg ttccattacc ctgtttggag
     4081 agaaagggac cgtaaaagtc ggtggccaat atttgaacct cttggaatac cagtcgattg
     4141 atggctatga gatcatgggt cttgagccgg ggaatccccc aaacgtatat ggcaagaata
     4201 ccgggtcgat gtcaaatcat gaaaaagtct atgagaacgt gattgatgtg ctggtccatg
     4261 ggggaagtat tacgacagat ctactagatg ggttgaagac tgttcagatt attgaaaaga
     4321 tctataaggc tgccatgaaa aggtagtgtt tctgaggatg tgtatatgag gaatagaccg
     4381 acaattaaaa aagtgcagat aaaggatgtg acgtttggtg agaatgtcac ggttgtagaa
     4441 ccggtgaacc tctatgaatg cgagatcggt gatgattgtt tcattggtcc gtttgtggag
     4501 atccagcgga acgtcaagat agggaaaaac tgccgggtgc agtcgcacgc gtttatctgt
     4561 gagcttgtgt cgattggtga tttctgtttt atttctcatg gggtgaagtt cgtcaatgat
     4621 ctattttcgt tgggcaaacc agctggtggc gatcggtcgc tgtggaggtc gacgaaggtt
     4681 ggtagttacg tgtcgattgg gacgaatgcg acgattctcc ccgtgataat taccgataat
     4741 gtggtgatcg gcgcgggctc ggtcgtgacg aaggatatca aagaatccgg ggtgtatgca
     4801 gggaaccctg cgcggaggct acggtctttg taggagttgt ctgtgatgaa cattccactc
     4861 gtggatttga aggtaaatta tcattctata aaaaaagaga ttgatagtgc tattaaggat
     4921 gtactggaga gtagttcgtt tattatgggg ccgtatgtga aaaaattcga ggaggatttc
     4981 gccaggttct gccaagtgaa acatgggata gggtgtgcga atggaactgc ggctctccat
     5041 cttgcattgc tggctgcggg gattaaaaaa ggcgatgagg ttctcaccgt ccctaatacg
     5101 tttattgcga cgacagaatg tatctcctat gtcggtggta agatcaaatt cgttgatgcg
     5161 tctccgcaga cggcactgct tgatatcgat gcgttggaga aagcgattac tcggaagacg
     5221 aaggcactca tcgtggtgga tttgtacggg cagatgcctg atatgcaacg gatccgggag
     5281 atcgcggtga aaaatgattt gttcgtcatt gaggatgcag cgcaggctca tgccgcggag
     5341 tggaacggcc atcaacccgg gttttacggg gatatcgcgt gtttcagctt cttccctgca
     5401 aagaaccttg gatgctatgg tgatggtggg gcggtgatga ccaactatga tgagc
//
LOCUS       DATMXJ010000096         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33343, whole genome shotgun sequence.
ACCESSION   DATMXJ010000096 DATMXJ010000000
VERSION     DATMXJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33343"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VN377_02705"
     CDS             <1..960
                     /locus_tag="VN377_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWR27329.1"
                     /translation="YCARDRNGVTPLAIGQKAGEVAVASETCAFPNLGFSIKKYMTPG
                     EIIFLSKKGVEEKKKGRTGTKICAFLWVYTGFPASSYEGINVEKVRENCGTFLAKRDT
                     VKADLVSGVPDSGVAHAVGYAMESGIPYRRALLKYTPGYGRSYTPLTQELRDRIALMK
                     LMANDAIAKGNKIILCEDSIVRGTQLKNYTIAKLMQAGAKEVHVRPACPPLMFPCVYN
                     LSTRSIQELVARRAIRALEGKDIDDVSEYVDPSSEKYKRMVDWIAKDLGATSLRYQLL
                     DDMVQAIGLPKEKLCLYCWIGRGVQKNLQSYQEAQNQEMLCCK"
     gene            1139..1909
                     /locus_tag="VN377_02710"
     CDS             1139..1909
                     /locus_tag="VN377_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWR27330.1"
                     /translation="MGNKSRSCPIFLSIIVLGFLPILLAPSIQAADYYADLTITVDFS
                     GFVSIAGLTNYPNLTIQNTEQYTSKQQSYWLLNITKQETFSEFVYDLTLPKGSSISYM
                     KSSGSLRIEENLGSLIIRGFGENESLSILIQYQTEKQGGSILQDTIIYLILLPAILLV
                     TLLILFFYLKEKKTRGLPSEAVIKEPLGDMKGLNHRQKQIMQLLHERNIPLTQTDIQK
                     ELEIPKASVSRNIHGLERKGLIEKEQIGMSNLIRLKKQ"
     gene            2028..>2694
                     /locus_tag="VN377_02715"
     CDS             2028..>2694
                     /locus_tag="VN377_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27331.1"
                     /translation="MKKMTNLLLVLMLLGAIVSLAFAVEADDNNQGNPIHGNENDDQT
                     NETNSSHGHHVDNETEHEVGIMNNSLGARIRLLQLEKALLTNILKGAMTVQVLKGLNV
                     TTTELEVILGNLSDVLVAVRAADPAANDSVLVFVQLKNESKNLTKQFRETLRLLLDNE
                     TIAMIKEQLRNMTSDELQNCSKRLTHWIRQFNQNQLYRLYGLIGETNTTLINDYIHGN
                     ISLD"
BASE COUNT          852 a          614 c          601 g          627 t
ORIGIN      
        1 tattgtgcca gagaccgtaa tggagtcacc cctcttgcga tcggacaaaa agcaggggaa
       61 gtcgcagtcg ccagtgaaac ctgtgcgttc ccaaatctgg gatttagcat aaaaaaatat
      121 atgacccctg gagaaatcat cttcctgagc aaaaaaggag tcgaggaaaa aaagaaggga
      181 cgaaccggaa caaagatctg tgcgttttta tgggtgtaca ctggttttcc cgcatcatcc
      241 tatgaaggga tcaatgtgga gaaagtacgg gaaaactgcg gtacgttttt agcaaagcga
      301 gatacagtga aagcagacct tgtctcagga gtccctgatt caggggttgc tcacgcggtc
      361 ggatatgcga tggaatcagg catcccgtat cgaagagcct tgttaaagta cacgccggga
      421 tacggtcgga gctacacgcc gcttacccag gagctcagag atcgcatcgc gctcatgaaa
      481 ttgatggcaa atgatgcgat agcaaagggg aataaaatta tcttatgtga agattcgatc
      541 gtccggggga cccagttgaa gaattatacg attgcgaaac tgatgcaggc gggtgcaaaa
      601 gaggtccatg tacggccagc gtgtcctcct ctgatgttcc cctgtgtcta caatttatcc
      661 acaagaagca tccaggagtt agtcgcccgg agggcaatcc gggcgctgga aggaaaagat
      721 atcgatgatg tatccgagta cgtcgatccc tcatcagaga aatataaaag gatggtcgat
      781 tggatcgcaa aagacctcgg agcaacctcc cttcgatatc aactattaga tgatatggta
      841 caagcgattg gattgccaaa agaaaaatta tgcctctatt gttggattgg gcgtggggtg
      901 cagaaaaacc tgcagtcgta tcaagaagcc cagaaccaag agatgctctg ttgtaagtaa
      961 tcatcagacc gtggtacatc aaaaaaggag tgaatgacgc aatactggat actggagaaa
     1021 tctttgatag tacttctcaa ctcttcatag ttttctcatt ggaagaaact ttaataataa
     1081 accatatcgt ttcaggaata agaaatgacc tcgcgaaaac agtccaagag gagctgcgat
     1141 gggaaacaaa tccagatctt gtccaatttt cttatcaata attgtccttg gcttcctgcc
     1201 aattttgctt gcgccatcca tacaagcggc tgactattac gcagacctaa caataacagt
     1261 ggatttttcc ggtttcgtca gcatcgcagg actgaccaat tatcctaacc tcaccataca
     1321 aaacaccgaa caatacacct caaaacagca aagctattgg ctattgaata tcaccaaaca
     1381 agagacattt tctgagttcg tctatgacct gacgctgcca aaaggctcct cgatcagtta
     1441 catgaaatca tctgggtccc ttcgcatcga ggaaaacctc ggaagcctca taatcagagg
     1501 atttggggag aacgaatcgc tctccatcct catccagtac cagacagaaa aacaaggcgg
     1561 ctcaattctc caagatacta tcatctatct catcttgctt cctgcaattc tccttgttac
     1621 cctcctcatc ctattttttt atctcaagga gaaaaaaacc aggggactcc catctgaagc
     1681 ggtgataaaa gaaccgttag gcgatatgaa ggggttaaat catcgacaga aacaaatcat
     1741 gcagctgctg catgagagaa acatccccct cacacagacc gacatccaaa aagaactcga
     1801 gatcccaaag gcatctgtct ccaggaatat ccatggattg gaacgcaaag gtctgataga
     1861 gaaagaacag atagggatgt cgaatcttat ccgtctcaaa aaacaatagt ttttctgtcc
     1921 catgtctcgg gttgttccgc tgtgttccgg tgaaactaga tatgctgttt cagccaatac
     1981 atacttacga gccatacgga aatggcacac gaaaaggagt taatgatatg aaaaaaatga
     2041 caaatctctt actggtgctg atgctactgg gagccatcgt ctctttggca ttcgcagttg
     2101 aggcagatga taacaaccaa ggcaacccaa tacatggaaa tgaaaacgat gatcaaacca
     2161 atgagacaaa ctcttcccat ggacatcacg tcgataacga gacagaacat gaagtaggaa
     2221 tcatgaataa ctccctgggt gctcgaatca ggctgctcca attggaaaaa gcgcttttga
     2281 caaacatcct gaaaggagcc atgaccgtcc aggttctcaa gggacttaat gtgacgacca
     2341 cggaactgga agtcatcttg ggaaatctca gcgatgtgct tgtcgcagtc cgggcggcgg
     2401 atcccgctgc aaacgactcg gtccttgttt ttgtccaact gaaaaatgaa tcaaaaaatc
     2461 tcacgaaaca attccgagaa accctgcggt tattactcga taatgagacc atcgcgatga
     2521 tcaaggaaca actcagaaac atgaccagcg atgagcttca gaattgcagt aaacgactta
     2581 cgcattggat cagacagttc aaccaaaatc aactctatcg actctatggc ctcattggag
     2641 aaacgaacac caccctcatc aatgattata ttcacggaaa tatcagctta gact
//
LOCUS       DATMXJ010000097        10447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33355, whole genome shotgun sequence.
ACCESSION   DATMXJ010000097 DATMXJ010000000
VERSION     DATMXJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33355"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VN377_02720"
     CDS             complement(<1..1429)
                     /locus_tag="VN377_02720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HWR27332.1"
                     /translation="MGKTIIQKIFENHSNTTAEINEIIDVTIDVRVARDFGGANVVKH
                     LEENHLPIHDPKHTFFTFDCNPGGCDQKYAENQQLCRVFARKHGIRIYDINSGIGTHL
                     AFDEGLVFPGDTFVSTDSHANILGALGAFGQGMGDIDIAYAFAYGKIWFKVPPSVKIV
                     LKGTPAKITTPKDVVLKINKELGANGLLGYAAELYGPYVDSLSLDGRLTIASMATEMG
                     GIIILFPTNAKIMDYYKNTFGITIKPVHADPDAHYERTIEIDISDLTPQIARPGHPED
                     VVSVSEMSGIPVDSGFIGSCTNGRLEDLRAAASILKERTIAPGRILKIVPATDMIWNS
                     CLKEGLLKIFKDAGALVGSAGCAGCAAGQIGQTGKGEVTISTGNRNFTGKQGKGDIYL
                     ASPQTVAASLLAGYITTIDAIPKKPAPMKIEIPSTKTIPEQKKEKMVSPFIVEGRAWV
                     IQKDNIDTDMIYHNRHLTITDPTEMG"
     gene            1728..4562
                     /locus_tag="VN377_02725"
     CDS             1728..4562
                     /locus_tag="VN377_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27333.1"
                     /translation="MKKLLVVVLTLMTCISMSGFGASLTNIFDQNNTKISSQNLITGV
                     KTSSTTMDQVKKSAYSYLNMQENPYNTDQENQNSTITAPWIKPASTLFEWWILVQYNG
                     NAFEKQLDISINDFKQKFLKHPYYCEDAFFNIDDDPEDDLKVSFGFYWESILNTKTNV
                     EHKSLEDMVRVRQINNGPSDLYAGLEVWSEIHVNWGLINAYSQNNEFSYYSNQLQPKQ
                     TQSPQPLRPFVSFLNILQQKIQHITRIGQALISNLLNRNILKTQEEPQQGQFTQNPQR
                     LAADNDYIAVGVGYRTLPGQRIPQYVEKRFAFAKEDIFSPIIFQQVMKTEPSGVDPLE
                     LLYGFRAYNGSTDALKYDIAFSVQFTPFVTLTTQFIPLGGYVYYRFDTESQHAGETMI
                     TYSSDIHVGDGDGVSLSLVFDQIDNTMAQPGRWMSFGLRRDPHGFDYEANAIFDVAVI
                     VDSPWFSQKVRINGLPMLLSYEWKIFDDFEVTFVQGELFYVNVRGYADVTMSSPIDEL
                     IVYYPKLRNPTDEDVPFLQMNNIPSSRKLEAKATLNIQNGSMLRIDADGYVDLTKSGS
                     LGDVTVFYPKANPEDPSVALMRVPAGSINSQRISAEATLYVDADNFSNINNYVYGKIQ
                     RQADSNFNELNLYLPNVAVPLVQITEIPANAYATGTFWWNQLKGFGRAERSSATEYPD
                     PVHFNLVFDTLTLSDVFSIGDGHAQTDFKIAQDGYFKFDTTHDILGNMFSVGNSATGN
                     SLSIGASTVSAQNFEAAWGLNTTGPQPQIESLALTGSLNALNDISVAIALDGEVVDFT
                     GDWSLGESGGFTIDLQQNADMRIDIIHLNNLSGRLDLNGYVILSGSFHYDMSWKWKQG
                     ESLQDPGYFKINEGTNEPNLKEIGFDFVYKDESSVDRWGADITLSNFAVYICVEWYWQ
                     DGFHIWPVIEVSGNLAFNALLNYNWYQVWP"
     gene            4593..7598
                     /locus_tag="VN377_02730"
     CDS             4593..7598
                     /locus_tag="VN377_02730"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27334.1"
                     /translation="MGKNTINKKYGRLVVVLFLVVCLMFSAAQALLVSTDQLNDFASR
                     TSSLTPHPLLQDNYYTWEDLFTDSTKVDPSMSYNYEITGGGASMKDTFPFWTDPAWTR
                     MKQVTITNNAGEILYDYALQILIPYDADMRPDYGDLRFKHESSGDVILNYWVETYDAS
                     SASVWVKIPYAPIGTSKMYLFYGNPAATSQSGFYGVFTAWEEYWPNDEQITYHGTIEG
                     AWDPDVSFGNNDFLVAWEEGQAYYPPYTWGFKQEIRASMYDSEGTRVVFDTLIYQDAT
                     LYFRNENPSIDYGAGKFFVAWEHYDTVANPSATTEDIKARTVVRNGNQLTLGSVINVC
                     SAADCQADANVQFDSVNNRFCVVWEDARNGESNYNIYGRLYDNNGNPVGGEKNICTAA
                     NTQCEPWVAFDPIHAQYMIVWEEGITPDLGPFSIKAGLFDSSLTQIGNTITIAIGTDG
                     MDYNYPCVEFSAQTQEYLITYNNDDISADDWWGNIWGTIYDDAGNVVVPIFQIKSGEF
                     TRTDIVPYLTSSFFVSFNSKGATGPSGLIWGKLVSSEGEVFTGDVQLSASTAAEADWA
                     NMAVGNDKVFVAWEDVRVYYPYPWNDMPDAYGNLWRFNIPSGFEMTYSMGDEKVLLLE
                     AQLTSIPIDPENLLAWYDFDAVFDGTISFDILNGAGDTVLLPGVSPGQSLQALDPVAL
                     RLRAHFTRSNPSYSPALDSWMVRFIGLDEEPPVTVLDHISGTQGLGGWYTSQGVTVWL
                     ASYDLPEGTGSGVNHTYYTLNDGSTQEYDDGSGISLVVTQDMHWMGIWEVTFWSVDHS
                     GNIEDSTPPDNTIMIRIDAERPYVEISEPVDEQQVNLPFWVRANASDNAVVDRVEFDI
                     EPFGQHTGLPYVDTEPPYEWLCNISEMNGEGLKSSDGGQSLGINKMVRARVYDQSGQV
                     WTDEHWVWINNAESFGKRFLIGFLKNRNISDSEISFTTRCILSLTLDTFIPKLYISNE
                     HFIVSQSNKIGYIGPLGIIGFFDTRVVGE"
     gene            complement(8292..8717)
                     /locus_tag="VN377_02735"
     CDS             complement(8292..8717)
                     /locus_tag="VN377_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27335.1"
                     /translation="MSCPHYQDFTRNCLKEYPTLIKYESIVRCESEDYPICILYHILQ
                     NDFRCKYLGSCSRSFPDEIPKFDELMRRDKSVYEIIVKQVHNYCLLQENHMQCARYKI
                     IEEGKQLLPGLIPDGNNINIMGPIITVPEKEMITNRNKT"
     gene            complement(8819..9046)
                     /locus_tag="VN377_02740"
     CDS             complement(8819..9046)
                     /locus_tag="VN377_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27336.1"
                     /translation="MMRRYLTNEDIQKIREEVLKGKSKHQVSREMNIIDLVVYKYTKD
                     LPNKYKREPYISGKPLELLKELLEKGYVGSE"
     gene            9177..>10447
                     /locus_tag="VN377_02745"
     CDS             9177..>10447
                     /locus_tag="VN377_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27337.1"
                     /translation="MNKNLKKGLVIGIVLLFIETCIIPTIAQKIVEKPSLPTSAGKTL
                     YVDDDNILGPWDGTLEHPFQYIQDAANLLKRGDTIYVFNGTYHVNVLRINHTSSIKLL
                     GEDKNTTTIIGEIWFDQTHGLTISGFTWQTLTPSPPDCLILSHSTNCTVSGNIFKPEN
                     FGMELAYLFNCNISSNTFELYGLTSPLAIAIGLSTQIIMNDNKFFGDPSSDPLLGQGI
                     QMGVAPRNTISRNLFMNLESAIQMGSGIPFTRHNLVSDNTIINTYVGIALGAPSTRNI
                     IKGNIINTSWDGIVLYDQATRNLVYQNKISYCSEFGIFGFWAFANDILANTISDNKYG
                     IFLGGPGGDIRCFFNRIAYNNIERNDYWNVIDFNGINLWYNLKNGKGNYWDNYTGIDA
                     NGDGVGDTPYAIPGCRNKDRYPLMEPVDINNV"
BASE COUNT         2863 a         2302 c         2389 g         2893 t
ORIGIN      
        1 gtcccatctc tgtcgggtct gtgatcgtta gatgccggtt gtgatagatc atgtcggtgt
       61 cgatgttgtc tttttggatg acccaggctc ttccttcaac gatgaacggg gaaaccattt
      121 tttctttttt ctgctcaggg atggttttcg tagatggtat ttcgattttc ataggggctg
      181 gtttttttgg gatcgcatca atcgtggtga tgtacccggc aagaagtgat gcggcgacgg
      241 tctgcgggga tgctagatag atatcacctt tcccttgttt ccctgtgaag ttcctgtttc
      301 ctgtcgaaat cgtcacctct ccttttccgg tctgaccaat ctgaccagca gcgcatcctg
      361 cgcagcccgc ggagccgacc agtgcccctg catctttgaa gatctttagc agaccttctt
      421 tgaggcagct gttccagatc atgtcggtgg cagggacgat ctttaggatt ctccctggcg
      481 cgatggtcct ctctttcagg attgatgctg ctgctcgcag gtcctccagt cggccattgg
      541 tgcaagaacc gatgaacccg gaatcgacag ggataccgct catttctgag acactgacaa
      601 cgtcctcggg atgacccggg cgtgctatct gtggtgtaag gtctgagatg tcaatctcga
      661 tggtgcgttc atagtgcgca tcaggatctg catggaccgg tttgatcgtg attccaaacg
      721 tgttcttata atagtccatg atttttgcgt tcgtcggaaa caaaataatg atcccgccca
      781 tttctgttgc catggatgca atggtaagcc tgccgtcgag cgacaaggaa tcaacgtaag
      841 ggccgtagag ctctgctgcg tatcctagga gtccgttcgc cccgagttct ttgttgattt
      901 tcaagacaac gtctttcggg gtcgtgattt tcgccggggt tcccttgagt acgattttta
      961 cggacggggg aaccttgaac cagattttcc cataggcaaa tgcataggcg atatcgatgt
     1021 ctcccatgcc ttggccaaag gcaccaagtg caccaaggat gttggcatgg gaatcggttg
     1081 aaacaaaggt gtccccgggg aacacaagtc cttcatcgaa ggcaaggtgt gtcccgatcc
     1141 cgctgttgat gtcatagatt cttatcccat gttttcgtgc gaacacgcgg cagagctgct
     1201 ggttctccgc gtatttctga tcgcaaccgc ctgggttgca gtcgaaggtg aagaacgtgt
     1261 gcttaggatc gtgaatagga agatggtttt cttctagatg tttcacgacg ttcgcaccac
     1321 cgaaatcgcg ggcgacccgt acatcgatgg tcacatcgat gatctcgttg atctctgcgg
     1381 tggtgttcga atgattctcg aaaatttttt ggatgattgt tttccccatg atccttttct
     1441 ctccagtgtt tttccaatga ttccctgatg atatcgcaca tcatgtgaga ctatgaaaat
     1501 gttgtggtga ccatcgcttt tcaccgttcg aaaaagtcga ttgtttttca tattgttttg
     1561 gtttgatgct atggtcatgt tgaagattct gaacgttttt tacgatttgc agctaaaaat
     1621 acgtgaaata gattaaaaaa tcacaacaaa ttatatatac taaatgctct taattatata
     1681 gagggcagaa agggtatatg atagaataag gaagaggtga tatgtttatg aagaaattgt
     1741 tagtggtggt actaacttta atgacatgta ttagcatgtc aggatttggt gcttctttaa
     1801 ccaatatttt cgaccaaaat aacacaaaga tttcctctca aaatcttatc acaggggtaa
     1861 aaacatcttc aacaaccatg gaccaagtaa aaaaatcagc gtattcgtac ctcaacatgc
     1921 aggaaaatcc ctacaacacc gatcaagaaa accaaaacag caccataaca gcaccgtgga
     1981 taaaacctgc tagcacactg tttgaatggt ggattctcgt acaatataac ggaaacgcat
     2041 ttgaaaaaca gctcgacatc tccatcaatg atttcaaaca aaaattcctc aaacacccct
     2101 attactgcga agatgcattt tttaacatcg atgacgaccc tgaagacgac ctcaaagtat
     2161 cctttggttt ctactgggaa agcatcctga acacaaaaac aaacgtggaa cataaatctc
     2221 ttgaggatat ggttcgagta cgacagatca acaacggtcc ttctgatctc tacgcaggat
     2281 tggaggtctg gtctgagatc catgtgaact ggggactcat caacgcttac agccaaaaca
     2341 atgaattctc ctattacagc aaccaactac aacccaaaca aacacagagt ccacaaccac
     2401 tgagaccatt cgtgtcattc ctgaatatct tacaacaaaa aatacagcac atcacccgta
     2461 tcggccaagc tcttatctcc aacctcctaa atcgtaacat cctcaagact caagaagaac
     2521 cgcagcaagg tcaattcacc cagaatccac agagactagc tgctgataac gattacatcg
     2581 ctgtcggggt cggttatcgt acacttcccg gtcaaaggat accacaatat gtagagaaaa
     2641 ggtttgcgtt tgccaaagag gatatcttca gcccaatcat cttccagcag gtcatgaaga
     2701 ctgagcctag cggggtagac cctctagagc tactttatgg gttccgcgcg tataacggca
     2761 gcacagacgc cctgaaatac gatatcgcat tctccgtgca gttcacccct tttgtcacct
     2821 tgacgactca attcatcccc cttggcggat atgtctatta tcgttttgac acggaaagcc
     2881 agcatgcagg tgaaacgatg ataacatact cctctgatat ccatgtcggg gacggggatg
     2941 gtgtcagcct ttcccttgtt tttgatcaaa tcgataacac catggcacaa ccagggagat
     3001 ggatgagctt tggtctacga agagaccccc atgggtttga ctatgaggca aacgcgatct
     3061 tcgatgtcgc agtcatcgtc gattcaccct ggtttagcca gaaagtacgc atcaatggac
     3121 tgccgatgct tctctcctat gaatggaaga tcttcgatga ttttgaggtg acattcgtcc
     3181 aaggagaact cttctatgtc aatgtcagag gatacgcgga tgtgaccatg agcagcccga
     3241 tcgatgaact tatcgtgtac tatccgaaac tgaggaatcc cacggatgag gatgtgccat
     3301 tccttcaaat gaacaacatc cccagctcaa gaaaactaga agcaaaagca accctgaaca
     3361 tacagaacgg aagcatgttg cggatcgatg cagatgggta tgttgatctg acaaagagtg
     3421 gctcccttgg ggacgtcacc gtgttttacc caaaagcaaa tcctgaagat ccatccgttg
     3481 ccctgatgag agtgccagcg ggaagtatca acagtcagcg catcagcgct gaagctacgc
     3541 tgtacgttga tgccgataac ttcagcaaca tcaacaacta cgtgtacggg aaaatccagc
     3601 gacaggcaga ttcgaatttc aatgaactga acctgtacct tccgaacgtt gcagtaccac
     3661 tggttcaaat cacagaaatc cctgccaatg cctatgccac cgggacattc tggtggaacc
     3721 agctcaaggg gttcggacgg gcggagcgat cgtcagccac tgagtaccca gaccctgttc
     3781 atttcaacct tgtctttgat actcttaccc tcagcgatgt cttcagtatc ggcgacggac
     3841 atgcccagac agattttaag atagcccagg acgggtactt caaatttgac accacccatg
     3901 atatcctggg caatatgttt tccgtaggga attcagccac cggcaattcg ctttccatcg
     3961 gtgccagtac tgtatcagcc cagaactttg aggccgcatg ggggttgaac accaccggtc
     4021 ctcagccaca gatcgaaagt ctggcgctta ccggttcact gaatgcatta aatgatataa
     4081 gtgtcgccat cgctctcgac ggcgaggtcg ttgatttcac cggggactgg tcacttgggg
     4141 agtccggtgg ttttaccatt gatctgcaac agaatgcgga catgcgcatt gatattatcc
     4201 atctgaataa ccttagtgga cggcttgatt taaacgggta tgtcatcctc agcggcagct
     4261 tccattatga tatgagctgg aaatggaaac aaggggaaag tctgcaggat ccagggtatt
     4321 tcaagatcaa tgaaggaacc aacgaaccga atcttaagga aattgggttt gactttgtct
     4381 acaaggacga atcaagcgtc gatcgatggg gtgcggatat cacactgtct aatttcgccg
     4441 tatatatctg cgtcgaatgg tactggcaag acgggttcca tatctggccg gtcatcgagg
     4501 taagcgggaa tcttgcgttc aatgcgttat tgaattataa ctggtaccag gtatggccct
     4561 aggtacggtg gagaaaataa aggtgtatga acatgggaaa aaatacaatc aataaaaaat
     4621 atggtaggct agtcgtggtc ttgtttcttg tcgtatgttt gatgttttct gcagcgcagg
     4681 cattattggt ctcaaccgat caattgaatg atttcgcatc aagaacatca tccttgaccc
     4741 cccatcccct ccttcaggac aattactaca catgggagga tttatttacg gattcgacga
     4801 aggtagatcc ttcgatgtcc tataattatg agatcactgg tggtggtgct tcgatgaaag
     4861 ataccttccc cttctggact gatccggctt ggacacggat gaaacaagtg accatcacca
     4921 acaacgccgg agagatactc tatgactatg cactacagat ccttatcccc tatgatgctg
     4981 acatgcggcc tgattatgga gacctccgtt ttaaacatga aagcagcggg gatgtaatcc
     5041 tcaattattg ggtcgaaacc tatgacgcaa gcagcgctag cgtctgggtc aagatccctt
     5101 acgcaccgat tggaacaagc aagatgtacc tgttttatgg gaaccccgcg gcaacaagcc
     5161 agagtggttt ctatggtgtc ttcaccgcat gggaagagta ttggccgaat gatgaacaga
     5221 ttacgtatca tgggacaatc gagggtgcat gggatccaga tgtgagcttc gggaacaatg
     5281 acttcctggt cgcctgggaa gaaggacaag catactatcc accctacacc tgggggttca
     5341 aacaggagat tcgggcatcc atgtatgact ctgagggcac acgggtggtt tttgatacgt
     5401 taatctatca ggacgctacg ttatacttca gaaatgagaa cccgtcgatc gactatggcg
     5461 ccgggaagtt ctttgtcgct tgggaacatt atgacacggt cgccaaccca agtgcgacaa
     5521 ccgaggatat caaggcacga acggtggtcc ggaacggcaa ccagttaacc ctgggcagcg
     5581 tcatcaatgt gtgcagcgcg gctgactgtc aagcggatgc gaacgttcaa ttcgactcgg
     5641 tgaacaaccg gttctgcgtc gtatgggagg acgctcgaaa cggggagagc aactacaata
     5701 tctatggtcg gttgtacgac aacaatggaa acccggttgg cggagagaaa aacatctgca
     5761 ccgcggcaaa cacccaatgc gaaccatggg tcgcgttcga tccgattcac gcccagtaca
     5821 tgatcgtctg ggaagaggga atcacaccgg atcttgggcc ttttagcatc aaagcaggac
     5881 tctttgatag ttctctgacg cagatcggca acacgatcac gatagcgata ggcaccgatg
     5941 gtatggatta taattatccc tgtgtcgagt tctcagcaca gacccaagag tacctgatca
     6001 cctataacaa tgatgatatc agcgctgatg attggtgggg taacatctgg ggtacgattt
     6061 atgatgatgc aggaaatgtt gtcgtcccga tattccaaat caagtctggg gagttcaccc
     6121 ggaccgatat cgtcccgtat cttacctcat cgttttttgt ttctttcaac agcaagggag
     6181 ctactggtcc ctcaggcctt atctggggga aactggtatc ttctgaaggt gaggtgttca
     6241 cgggtgatgt tcaactttcc gcaagtaccg cagcggaagc cgactgggca aacatggctg
     6301 ttggaaacga taaggtgttt gtagcctggg aagatgtccg ggtctactat ccctatccct
     6361 ggaacgacat gccagacgcg tatgggaatc tctggcgttt caatattcca agtggctttg
     6421 agatgaccta ttcgatggga gacgaaaaag tacttctttt agaagctcag cttacgtcga
     6481 ttccgatcga cccggagaac ctcttagcct ggtatgattt cgatgcggtc tttgatggta
     6541 cgataagctt tgatatattg aatggggcgg gggacacggt tcttcttcca ggcgtaagcc
     6601 cggggcagtc cctgcaggcg ctcgatcctg tcgccttacg cctccgtgct catttcacaa
     6661 ggagcaatcc ttcctattcg cccgcattgg acagctggat ggtccgtttc atcggccttg
     6721 atgaggaacc accggtgacg gttcttgatc atatctcagg cacgcagggt ctgggcggat
     6781 ggtacaccag ccaaggcgtc accgtatggc ttgcctccta tgatcttcct gagggcaccg
     6841 gaagcggtgt caatcatacg tattatacgc tcaatgatgg atcaacgcaa gaatacgatg
     6901 atggtagcgg tatttcactg gtcgtgacac aggacatgca ctggatgggg atctgggagg
     6961 tcaccttctg gtctgtggac cattcaggga acattgagga cagcacaccg cctgataata
     7021 ccatcatgat acggatcgat gcagaacgcc cctatgtcga gatttcggag ccggtggacg
     7081 agcagcaggt caacctgccg ttctgggtcc gggcgaatgc ctctgacaat gcagtggttg
     7141 atcgcgtcga gttcgatatc gagccgttcg ggcagcacac gggtcttccg tacgtggaca
     7201 ccgagccgcc ctatgaatgg ttgtgcaaca tctctgagat gaacggggaa ggattgaagt
     7261 catcagacgg ggggcagtcg ctgggtatca ataagatggt ccgcgcccgg gtctatgatc
     7321 agtccggtca ggtctggacc gacgagcatt gggtatggat caacaacgcg gaaagtttcg
     7381 ggaaaaggtt cctgatcggc ttcctgaaga acaggaatat ctccgatagt gagatttcct
     7441 ttacgacccg ctgcatcctc tccttgacgt tggatacgtt catccctaaa ttgtacatat
     7501 caaatgaaca cttcattgtt tcccagagca ataaaattgg gtacatcgga ccgctgggga
     7561 tcattggttt ttttgataca cgagtggtgg gcgagtaagg gacgtactct tttacttccc
     7621 tctttttttt ttcttaaaaa acagtttgca aatctaaaat gcacagattt ctatcagcca
     7681 tgcaaattaa tataaaagaa caattagata gtaacgctta cgtgatggcg aggaactatg
     7741 gtgtgccccc tctatgttga ttttacacgg atgtgtcttg acacactccc tatgctagtc
     7801 aactttacaa ctcttaaaac ctgtaaatca gagcaatatg ttgattgtcc agtttacctg
     7861 gtatgcacga gcagttttaa ttgcgagtat tttcctcgtt gttcaaaaca atacagcgaa
     7921 aaattgccga aattagtcat ggatattttc atgacaaaag ctgcatttga aatcttgaaa
     7981 gacacgtggg tgaattactg tttatcacca gagaactcga aaacctgtgc gaaatataca
     8041 gtctattcca aaggggaaat accaccttta gaactacaac cggatggcag tataatgagt
     8101 ccatttgatt ttatatttaa aaggaaacat atcatacgtc caccagggta aacaacagat
     8161 gatatgaatc cgttgattct atcttaaaaa caatatctac tttgaaaaaa agacagggac
     8221 aaaaaactat actctatgtt taaaacaggt tgcaaaccta gattatacga ttatttatta
     8281 taatttcaat attatgtttt attccgattg gtgatcattt ctttctcagg aactgttatg
     8341 ataggtccca taatatttat gttattacca tcaggaatta agccaggtag taattgtttt
     8401 ccttcttcta ttattttata ccgtgcacat tgcatgtgat tttcttgtaa taaacaataa
     8461 ttgtgcactt gttttactat tatttcataa acagatttat ctcttctcat caactcatca
     8521 aattttggta tctcatcagg aaaactcctc gaacaggaac caagatactt gcatcgaaaa
     8581 tcattttgga ggatatggta gagaatacat ataggataat cttctgactc gcatcttacg
     8641 atactctcat attttataag agtaggatat tcttttaagc aatttctggt aaaatcttga
     8701 taatgtggac agctcatgtt ttttctttac caccgcaatt catatcactt taaacagtat
     8761 aaatgcttcg catttttcac catatcagat ttgacagact agatttgcca tacgagtttt
     8821 attcagaacc gacgtatcct ttctctaaaa gctcttttag gagttcgagt ggttttccac
     8881 tgatgtatgg ttctcgtttg tatttatttg gaaggtcctt cgtatacttg tacacaacta
     8941 aatcaatgat gttcatttct cgtgatacct gatgcttcga tttacctttc aggacctcct
     9001 ctcgtatttt ctgaatatcc tcgttggtaa gatatcgtct catcataaat gagtgtaaaa
     9061 agacctataa atagatattg aaaaaaagag ctgcaaatcc ttctattttt acattatttt
     9121 taaaataaat ttaaatacat gatatgcctt agcaatacat acggaggaaa taaattatga
     9181 acaaaaacct aaaaaaaggt ttggtgattg gaatcgttct tctttttatt gagacatgta
     9241 ttattccaac tattgcacaa aagattgtag agaaaccatc cttgccaact tcagctggta
     9301 aaacactcta cgttgatgat gataacatcc ttggtccttg ggatgggact ctagagcatc
     9361 catttcagta cattcaggat gctgcgaatc tattgaagcg tggtgatacc atctatgttt
     9421 ttaacggaac ataccatgta aatgttctga gaattaatca tacgtcctca ataaaactac
     9481 ttggtgagga taagaataca acgactatca ttggtgaaat ttggttcgac caaacgcatg
     9541 gattaacgat cagcggtttc acatggcaaa ctctcacccc ctcgccgccg gattgcctta
     9601 tcctcagtca ttcaacaaac tgtaccgttt ccggaaatat ttttaaacca gaaaactttg
     9661 gaatggaatt agcgtactta tttaattgca acatttcatc aaatacattt gaattatatg
     9721 gtcttacttc cccgctggca atcgctattg gtttatccac acagattatt atgaacgaca
     9781 ataagttctt tggagatcca tcatcggatc ctttgcttgg acaggggatt caaatgggtg
     9841 ttgcaccgag gaataccatt tcaaggaatc tttttatgaa tctcgagagt gccatccaaa
     9901 tgggtagcgg gattcctttc acaaggcata acttggtttc ggataacaca attatcaaca
     9961 catatgttgg gatagcctta ggtgctcctt ctacgcgtaa catcatcaaa ggaaatatta
    10021 taaacactag ttgggacggt attgttctat atgaccaggc aactcgtaat ctcgtttatc
    10081 agaataaaat ttcgtattgt agtgagttcg gaatatttgg tttttgggcg ttcgcaaacg
    10141 atattcttgc gaatactatt tcagataata aatatggcat ttttttagga ggtcccgggg
    10201 gagacattag gtgttttttc aaccgtattg cttataacaa catcgaaaga aatgattatt
    10261 ggaatgttat agactttaat ggtataaatc tctggtataa tcttaaaaat ggaaagggga
    10321 actattggga taactacact ggcatagatg caaatggcga tggtgtcgga gatacaccat
    10381 atgctatacc aggttgcaga aataaggatc gatatccctt gatggaacct gttgatatta
    10441 ataatgt
//
LOCUS       DATMXJ010000098        10108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_49172, whole genome shotgun sequence.
ACCESSION   DATMXJ010000098 DATMXJ010000000
VERSION     DATMXJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49172"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..4997
                     /locus_tag="VN377_02750"
     CDS             165..4997
                     /locus_tag="VN377_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27338.1"
                     /translation="MNDLDGAVIVNLALNYQNHSGTVTIGDFTITDNRFLLNDSQAYA
                     VLYNDNSIKRLHDGLISIGTFNFSENTLYKNNGGNNGVLFAGSLTYLQNDDVTVEDII
                     VSHNTVKDQTNPAFSLQQYTVENWSGTTTGMFGDIFVLDNIINSSFVNSDGIQISEIT
                     WGDLSDEASLTVGDCRIEGNQVTVTDGYAIVFYMDDIGNELQNNAQVIVGQVSISTNI
                     LTAGSGLLIDYFQCGDNLYQDSSCTLGDFQIVNNIIESTEYGVHIQQFSYLGYELNDN
                     ATFTMGDVLLNNNQIQSGSHGIYFSQLLIGENLFDSSIFSIGNITMNDNKIDSGGDGI
                     LFIDNVSSFRLGNSMNENSVVSLYDIEISRNTITDSDSGVSIGPCVFGGENDHNAFDA
                     FTVSNNSISFCSIGLETKDFSISDWCQPVIKNNTIDNCNVGIWFDSSYGSLIYNNYFD
                     NTLNAKDDTENTWNVIKTAGRNIIGGLYLGGNFWSDYTGIDGDDDTLGDTLIPYNSKG
                     NISTGGDLRPLTHNSEDLTLTQPTDFTATVVSPNQIDLSWIKGIHAVYTRVMQKIGGY
                     PVSISDGSLVYNGTGVSCSNASLSAGIHYYFRAWSWNDSTKLWSTTNASMSNTTWIVP
                     LAPTGFTATTVDTDEINLTWTKGSYATNTRIQRKIGSYPENISDGAHVYNGTSNIKSD
                     TSLSEDTQYYYRAWSWNSTSRLWSSTNVSARNTTWIFPLAPTNFRANTVGVNKIDLSW
                     TKGTHAVYTRVMQKTGGYPVSISDGSLVYNGTGVACSNASLSAGVHYYFRAWSWNSTN
                     KLWSSTNVAVSNITWNIPLAPTGFTAATIGSNQINLTWNKGSYAGYTRIQRRTTGYPV
                     SISDGTFVYNDTGSSHPHTGLSVNTTYYYRAWSWNTTSKTWSTTNASVFNSTWTGPLA
                     PPSLSTTTVSTTQIDLSWTRGFRATHTRVMQKIGSYPVSVSDGSLVYNGSDEVCSNSS
                     LSPGVRYYFRAWSWNSSSGLWSTTNASGSNRTWIVPLAPTGFTATTVDTDEINLTWTK
                     GSYATYTRIQRKIGSYPEDISDGAPVYNGTSNIKSDTGLTEDIQYYYRAWSWNSSSGL
                     CSTANASVSNTTWSNVLAPTGLSATTIAIDKINITWIRGTHATYTRLMQKTSGYPMST
                     SDGSLVYNGTGEACSYSNLSLETRYYFRAWSWNDSSGIWSTTNTSASNITGPFAPTGF
                     TATAISNNQINLAWSKGTYANYTRIQRKTGNYPMNISDGINVYNDTGSSYSNTNLSMS
                     TRYYYQAWSWNSTLRVWSINYANAQATTQGSGGGGFPPPSSENEAPTADAGGPYTGYV
                     NQTITLNAAGSSDDVEVVGYRWDWTNDGTWDTDWLPSSKTSHIYTTSGNYTINLQVQD
                     VEGLTDNDATIVIVNTGISPHQAPVADAAGPYRGLTYQEILFDGSRSYAINTSIVNYT
                     WVFGDGTYGYGVAPVHAYDSAGAFTVILTVNDSNDLKAIDTTLVTIDLDANRNNISDI
                     IELSIGEVITQDDLHAVSINGIWYYLVDTNHDGIYNAFYNPTTNKKTVLGEQDGKQLI
                     DIDGDGHWDYVYDSILGTATPYVEIISPLESPWFSVAVSVIIFIVILLVVWLYKTGRI
                     "
     gene            complement(5117..6442)
                     /locus_tag="VN377_02755"
     CDS             complement(5117..6442)
                     /locus_tag="VN377_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27339.1"
                     /translation="MPEPKNPEKNKNQPGRVIRVAILAEELLGWGSGKRMFPEILQNY
                     SWTTQGKSFSVTTRFIFDNDILQGRLTTKEFDVLLVPGGGVGDGQAIMKGFHYIKSVS
                     KWKKNIATFIQDGGGYIGICGGTALITDLKTAEKKPRTFLERQYNKSALGVTCVSSYY
                     KSLAFPLLYPFQKNHPENIGATAYVFSFAPGTTIDGARIHSGGAPIDFQIRKNHPIFS
                     DVKQDTERIRWWGGPALIVPQHPEREVHILAQYPAQDISDNTNLRIHAWRYTGGIHGF
                     FSAFFKAARYIKKRNDSLKNLFLYTYYLAGNWELTDKIIDLDYSNKPCITAEIYPNEN
                     KGRILLCAAHPEYMIWWNGHITEKKSEDFTCLGTGLYQWEDIASLSKDMSVEVAHSWW
                     MVRRFVAWAAKVPDTDLPPIERPVLTKEQSFLSKKNLWDGSLQNQMDNI"
     gene            complement(6448..7578)
                     /locus_tag="VN377_02760"
     CDS             complement(6448..7578)
                     /locus_tag="VN377_02760"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWR27340.1"
                     /translation="MKTKNKQHTQMHHPTTQHPLFGISTRQWVQLVKKNGGIDHKYLH
                     RALFITMTSVITAPIRMLFKIKYEKKINDVPLLESPIFIIGHWRSGTTYLHELLSNDP
                     RFCYTTLWSTLLPESGLILEPMKRFLARFLPSERPMDAIKVEMDGPYEDEAALAVLYP
                     WSFFHCLHFPRNAEEQYLKSIHFQGLTPQEKDQWKKTYLKFIKTVMFMNPGKRFLSKN
                     PPNTARISTLLELFPEAKFIHIYRSPYLVYLSTKKMRMKVLDKLALQEASEEEIEQQV
                     VSNYIRLMKSFFEQKEQIQTDRFVEIKYEDLIVDPMKHIQYIYETLRIPGLETAMPHM
                     MKYLERQSGYKTNVYSIDEKIVNHVDKNWKFTIDRWRYSPPK"
     gene            complement(7604..8704)
                     /locus_tag="VN377_02765"
     CDS             complement(7604..8704)
                     /locus_tag="VN377_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27341.1"
                     /translation="MTHKKDFKIFYQVPFQVYHDNPFWVAPFWYEVNGFFKKKNPFWS
                     HAECQLFIARKQGKIIGRIAAIIDNAYCEAVGEKIGYFGFFECIEDYECAEVLFQVAQ
                     DWLVSKKMRIMRGPIDGRIDIGCGFLLFGFDSLPSVLSTYSPAYYLSFAEKYDLKKSR
                     DFYHYSIDLTKPLPKELEDKARQSLSFGIQVRSFHRLRTEKELKWWSTLFLETFSEHW
                     GFVPVTPEEVKTRFGVKQLRWFVDQKLFLIAESNGSPVAYLWSTPEYNQVFHDMHGRL
                     NPFELLRFLITQRKINAGKLHFIGIKKEFRHQNIGSLLNYEALVEMKKRGYVRAEVGW
                     IDEQNSIAHMTIAHTGATLYKKHRVFEKDLSL"
     gene            complement(8981..>10108)
                     /locus_tag="VN377_02770"
     CDS             complement(8981..>10108)
                     /locus_tag="VN377_02770"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HWR27342.1"
                     /translation="NYGVVGDHPELVDINYATSTQYDWMHTNSIDYNVDFDQILISIH
                     NFNEIWVIDHSTTTEEAAGHTGGNSGKGGDLLYRWGNPMTYDAGTTNDRKLFSQHDAS
                     WIDEGCPGEGDILVFNNGANRPGNHYSTIDEITTPVDENGEYYLEPGSSYGPAAQTWI
                     YTANPPTSFYASHISGAWRLPSGNTLITNGESGLVFEVTPEGSTVWQYNTGGQVFKVV
                     YIPPWEPEPPENGTTPDLDCSGSLSWTDIDPGATVVGSFDVRNIGDSNSLLNWTVNTS
                     SISWGTWSFNPESGVRLTPEDGSVTVHVSVVAPNESEQTFQGFLRVENINDPTDFDTI
                     PVTLTTPVKIVPPWMTFLHQLLSYFLHHHPILETVVNNFIS"
BASE COUNT         2819 a         2098 c         2228 g         2963 t
ORIGIN      
        1 cgataggact gttctgtaac cattctggat taacagtatt gaataatacg ttttggttcg
       61 atgatcatag catcgatttt aattacactt caaatttcaa tactgatact gattattgtc
      121 tttacgataa tacaattgtt ggaaatcttt ttttgataaa tacaatgaac gatttagatg
      181 gtgctgtaat agtgaacctt gcattgaatt atcaaaatca tagcggaaca gtcacaattg
      241 gagacttcac catcactgat aatcgttttc tgttgaacga ttcccaggct tatgcagtgc
      301 tttataatga caattctatt aaaagattac acgatggtct gatttccatt gggacattca
      361 atttttcaga aaacacccta tataaaaata atggtggaaa taacggagtt ctattcgctg
      421 gttcacttac atatcttcag aacgatgatg tgacggtaga ggatatcatt gtctcacata
      481 acacggtcaa agatcaaacc aatccagcat tttctctgca acagtacacg gttgaaaatt
      541 ggagtgggac gacaactggg atgtttgggg atatcttcgt tctggacaat atcataaatt
      601 cttcttttgt aaattccgat gggattcaga taagtgaaat aacctggggg gatctttcgg
      661 atgaagcatc gctaacagta ggagattgtc gtatagaagg aaatcaggtt actgtcaccg
      721 atggatatgc gattgttttt tatatggatg atataggaaa cgagttacaa aacaatgcac
      781 aagtaattgt gggtcaggta agcatctcga ccaacatcct tactgcaggg agtgggttac
      841 taatcgatta ctttcaatgc ggggacaatc tctatcaaga ctctagttgc acactaggtg
      901 atttccagat cgtaaataac atcattgaaa gcactgaata tggtgtgcat atccagcaat
      961 tctcgtacct tggttacgag ttgaacgaca atgcgacctt tacgatggga gatgtcctac
     1021 taaataacaa tcagatacaa agtggttcac atgggattta tttttcacag ctcttaatcg
     1081 gggaaaatct cttcgactct tcaatatttt ctatagggaa catcacgatg aatgacaata
     1141 aaattgatag tggtggagac gggatccttt ttatcgataa cgtctcatcc ttcagactag
     1201 gaaattcaat gaacgaaaac tctgtcgttt cactatacga cattgaaata tcccgtaaca
     1261 cgattaccga ttctgattca ggagtatcca ttggtccctg tgtgttcgga ggggaaaacg
     1321 accacaacgc ctttgatgct ttcacggttt caaacaattc gatttcgttt tgttctattg
     1381 ggttagaaac aaaagatttt tcgatctcgg attggtgtca acctgttatt aaaaacaata
     1441 ccattgataa ttgtaatgta ggaatctggt ttgattcatc ctatggcagt cttatctaca
     1501 ataattattt cgacaacact ctgaatgcca aggatgacac cgagaacaca tggaatgtca
     1561 taaaaactgc tgggagaaac atcatcggcg ggctatatct aggtgggaac ttctggtctg
     1621 attacacagg catagatgga gatgatgata cccttgggga tacgctcatc ccctataact
     1681 ccaagggaaa tatctcgacc gggggtgact tacgtccctt aacacacaac tctgaggatc
     1741 tcacactaac acaaccaact gattttaccg caacggttgt cagccccaac cagatcgatt
     1801 tatcctggat taaaggaatc catgcggtct acacccgggt gatgcaaaag attgggggtt
     1861 atccagtaag tatttcagat ggttcactag tctataatgg gacaggtgtc tcctgctcta
     1921 atgcgagtct ttctgccggg atacattatt atttccgggc gtggtcctgg aatgactcta
     1981 caaagctttg gtcgacaaca aatgcgagca tgagtaacac cacgtggatt gttcctcttg
     2041 ctccgacagg ttttactgcg acgacagtcg atactgatga gatcaatctc acatggacga
     2101 aaggttcgta tgcaacaaat acaaggatac aaagaaaaat cgggagttat cctgaaaaca
     2161 tcagcgatgg ggcgcatgtc tataatggaa catcaaacat caaatcagat actagtctct
     2221 ctgaagatac acaatattat tacagagcat ggtcatggaa tagtacaagc cgattgtggt
     2281 catctacgaa tgtaagtgcg agaaacacga cctggatttt tccactagca ccaactaatt
     2341 ttcgtgcgaa tactgtcggc gtcaacaaaa tcgatttatc atggaccaaa ggtactcatg
     2401 cggtctacac cagggtgatg cagaagacag gtgggtatcc cgtcagtata tcagatggtt
     2461 ctttggtgta taatgggact ggtgttgctt gttctaatgc gagtctttct gccggggtac
     2521 attattattt ccgggcgtgg tcctggaata gtacaaataa gttatggtca tcaacgaatg
     2581 tggctgttag taacatcact tggaacattc cactcgctcc gactggtttt accgctgcaa
     2641 cgataggaag taaccaaatt aatttaacct ggaacaaagg aagttatgct ggttatacaa
     2701 ggattcagcg aagaacgaca gggtaccccg ttagtatttc agatgggacg tttgtgtata
     2761 atgatacggg ttcttcacac ccacacacag gtcttagcgt aaacacaacg tattattatc
     2821 gagcttggag ttggaatacc acaagtaaaa cctggtcaac gacgaacgcg agtgttttta
     2881 attccacatg gaccggtccc ttagctccgc ccagcttgtc aactaccacg gtgagtacca
     2941 cgcaaatcga tctctcatgg actagaggtt tccgtgccac acacacaagg gtgatgcaaa
     3001 agattgggag ttatccagtg agtgtttccg atggttctct agtgtacaat ggatctgatg
     3061 aggtgtgttc gaattcgagt ctttctccag gggtacgata ttacttccgg gcatggtcat
     3121 ggaatagctc aagtggacta tggtctacga cgaatgcaag tgggagtaac cgaacatgga
     3181 ttgttccact tgctccgacc ggttttactg cgacaacagt cgatactgat gagatcaatc
     3241 tgacatggac gaaaggttcg tatgcaacat acacaaggat acaaagaaaa atcgggagtt
     3301 accctgagga catcagcgat ggggcgcctg tctataatgg aacatcaaac atcaaatcag
     3361 atactggtct tactgaagac atacaatatt attacagggc atggtcatgg aatagctcaa
     3421 gtggactgtg ttcaactgca aatgcgagtg taagtaacac aacatggagt aatgtccttg
     3481 ctccaactgg tttatccgcg acaaccatcg ctatcgataa aattaatata acatggatta
     3541 gagggactca cgctacctat actagattga tgcagaagac aagcgggtat ccgatgagta
     3601 catcagatgg ttctttggtc tataatggca cgggtgaggc gtgttcttat tcaaacctat
     3661 cgctggaaac aagatattat ttcagagcat ggtcatggaa tgattctagt ggtatttggt
     3721 caacgacaaa cactagcgcc tcaaacatca ctggcccttt tgccccaaca gggtttacag
     3781 caacagccat cagcaataat caaatcaatc tcgcatggag taaaggaacc tacgcgaact
     3841 acacaagaat tcagcggaaa acagggaatt atccgatgaa catatcagat ggaataaatg
     3901 tctataatga taccggttct tcatattcaa acacaaatct gagcatgagc acacgatatt
     3961 attaccaagc ttggagttgg aattctactc tgagagtatg gtcgattaat tatgcgaatg
     4021 cacaagccac cacacaagga tctggtggag gagggtttcc tccgccctcg tcggagaacg
     4081 aggcaccaac cgctgacgca ggaggaccct atacaggata tgtcaatcaa actatcactt
     4141 taaatgcagc tggaagttcc gatgatgtcg aagtcgttgg atatcgctgg gattggacca
     4201 atgatggaac ctgggatacc gactggcttc cctcatcaaa aacctcacat atctacacaa
     4261 catctggaaa ttataccata aacctccagg ttcaagacgt tgaagggctc acagataacg
     4321 atgccacaat agtaatcgta aacacaggta tttccccaca tcaagcacca gtcgcagatg
     4381 cagcaggacc atatcgcggg ttgacctatc aagagatact atttgatggt tcgagaagct
     4441 atgcgatcaa caccagtatc gtcaactaca cctgggtatt cggggatgga acctatggct
     4501 atggtgtcgc tcccgttcat gcatacgatt ccgcaggggc tttcacggtc atccttactg
     4561 taaacgattc aaacgatctc aaagcaattg atacaacact cgtcacgatt gacttagatg
     4621 caaatcggaa taacatctct gatatcatag aactatccat aggagaggta atcacacaag
     4681 atgatttaca cgctgtatct atcaatggta tctggtatta tctcgttgat acgaatcacg
     4741 atgggatcta caatgctttt tataatccta cgacaaacaa aaaaaccgtt cttggagaac
     4801 aagatgggaa acagttgatc gatattgatg gcgacggtca ctgggattat gtgtacgatt
     4861 ctatcttagg tacggcgacc ccatatgtgg aaataatttc accgcttgaa tccccgtggt
     4921 ttagcgttgc tgtgagtgtg ataatcttta ttgtcattct gttggttgtg tggctctata
     4981 aaactggacg tatatgattg gaaaaacata aaacaaatcg agtcttgctg tttgttttaa
     5041 ggtttctttt acaacgagtg aaagatgttt ctacgaaatt tgcctaaaaa taataacctc
     5101 gtacacagta tatgatctag atgttgtcca tctgattctg aaggcttcca tcccaaaggt
     5161 tctttttcga tagaaatgat tgttcttttg tcaaaactgg tctttcgata ggtggaagat
     5221 cggtgtcagg tactttcgca gcccaggcta cgaatcttcg aaccatccac caggaatggg
     5281 ctacctcaac agacatatcc ttgcttaaag atgcgatatc ctcccattgg tacagaccag
     5341 tcccaagaca ggtaaaatcc tctgattttt tttctgtgat atgaccgttc caccagatca
     5401 tatattcggg atgtgcggcg cagagaagga ttctgccttt gttttcattt ggataaatct
     5461 ctgccgttat gcatggtttg ttggaataat cgagatcgat gatcttatca gtcagctccc
     5521 agttcccggc cagatagtac gtgtataaaa acaggttttt taaggaatca tttcgttttt
     5581 ttatgtatcg tgctgctttg aagaacgctg aaaagaaacc atggatgcct ccggtgtatc
     5641 tccatgcatg gatccgtaga ttcgtattgt ctgaaatatc ctgcgccgga tattgtgcca
     5701 ggatatgtac ttctcgttca gggtgttgtg gcactatcaa cgctggtcca ccccaccagc
     5761 ggattcgttc agtatcttgt ttgacatctg agaatatcgg atgatttttc cgaatctgaa
     5821 aatctattgg ggcgcctccg gaatgaatgc gagcgccgtc gattgttgtt cctggtgcaa
     5881 aagaaaagac atacgcggtc gcaccgatgt tctccgggtg gtttttttga aaaggataga
     5941 gcaacgggaa cgctagggat ttgtaatagg atgagacaca ggtcactccc agagcgcttt
     6001 tgttatattg tctctctaga aacgtccgtg gttttttctc cgctgtcttt aaatcggtta
     6061 tcagagctgt tccgccacag atcccgatat atcctccacc atcttggatg aaggttgcga
     6121 tgtttttttt ccatttgctg acacttttta tgtagtgaaa tcctttcata attgcttgac
     6181 cgtctcctac gcctccacct gggacaagta agacatcgaa ctcttttgtg gtgagacgtc
     6241 cttgtaaaat atcgttatcg aaaataaatc gtgtggtcac tgaaaaggat ttaccctggg
     6301 tcgtccagga gtaattctgt aatatctcgg gaaacattcg cttcccagat ccccatccca
     6361 acagttcttc tgcaaggatg gcgacgcgta tgacgcgtcc tggttgattt ttgtttttct
     6421 cagggttttt gggttctggc atgagtctca cttcggtggg gaatacctcc aacgatctat
     6481 tgtaaatttc cagtttttgt cgacatggtt cactattttt tcatcgattg aatagacgtt
     6541 ggttttgtat ccggattgtc gttccagata cttcatcatg tgaggcatcg ctgtctcaag
     6601 accagggatc ctcaacgtct catagatata ttgtatgtgt ttcattggat caacgataag
     6661 gtcttcgtat tttatctcga caaatctatc tgtttgtatc tgttcttttt gttcgaaaaa
     6721 actcttcatc agacgaatgt agtttgaaac gacttgttgt tctatttctt cctcggaagc
     6781 ctcttgtaac gcgagtttat ctaatacctt cattctcatt ttttttgtcg acagatacac
     6841 cagatagggg ctccgataga tgtgaatgaa ttttgcttct gggaacagtt caagcaacgt
     6901 tgaaatacgt gctgtgttcg gagggttttt cgaaaggaac cgttttcctg gattcataaa
     6961 cattaccgtt tttatgaatt tcaggtaggt ttttttccat tggtcttttt cttgaggggt
     7021 gagtccctgg aaatggatgg atttcaagta ttgttcttct gcgtttcggg ggaaatggag
     7081 gcaatggaaa aacgaccagg ggtataacac cgcgagtgca gcttcatcct catatggtcc
     7141 gtccatttct acctttatgg catccattgg tcgttcggag gggaggaatc gagccaggaa
     7201 tcttttcatc ggctccagaa tgagaccgct ctctggcaaa agagtactcc agagggtggt
     7261 gtaacagaac cgcggatcgt tacttaacag ttcatggaga taggtcgtcc cgcttcgcca
     7321 gtgtccgatg ataaagatag gggactccag gagaggtaca tcattgattt ttttctcata
     7381 ttttatctta aaaagcattc gtatcggggc ggtgataacc gatgtcattg tgataaagag
     7441 tgcccgatgg agatacttat ggtcaatacc tccgtttttt ttcaccagtt ggacccattg
     7501 tcgtgttgag atcccaaaca gggggtgctg agttgtcgga tgatgcatct gtgtatgttg
     7561 tttgtttttt gttttcattc ttgtcactct ttttatcagc gatttataag ctgaggtctt
     7621 tttcaaaaac acggtgtttc ttatacaatg ttgcaccagt atgcgcaatc gtcatatgag
     7681 caatcgaatt ctgttcatca atccatccga cttctgcgcg aacgtaccct cgttttttca
     7741 tctcgaccaa ggcttcataa tttaacaaag atccaatgtt ttgatgacgg aattcttttt
     7801 tgatgccaat aaaatgcaat ttccctgcat tgatttttcg ttgtgtgata agaaaacgaa
     7861 ggagttcaaa agggttgagc cgtccatgca tgtcatgaaa aacctggtta tactctggtg
     7921 tagaccatag atatgcgact ggcgaaccgt tcgattcagc gattaaaaac aacttttggt
     7981 caacaaacca tcgtagttgt ttgactccaa aacgggtttt cacctcctct ggtgtgacag
     8041 ggacaaaccc ccagtgctcc gagaatgttt ccaaaaaaag ggtactccac catttcagct
     8101 ctttttcggt gcggagtcga tggaacgagc ggacctgtat accgaacgat aagctttgtc
     8161 gcgctttatc ttcaagctct tttggtaatg gttttgttaa atcgatagaa taatgataaa
     8221 aatcacgtga ttttttcagg tcgtatttct ctgcgaagga tagataatag gctggtgaat
     8281 aggtggaaag cacactaggc agtgagtcaa agccgaaaag gagaaaaccg catccgatat
     8341 cgatacggcc atcgatggga cctcgcatta tcctcatttt ttttgacaca agccagtcct
     8401 gagcgacttg gaataatact tctgcgcatt cgtaatcttc gatgcattcg aaaaaaccaa
     8461 agtaccctat tttttctccg accgcctcgc agtatgcgtt gtcgataatt gcagcgattc
     8521 gaccgatgat tttcccctgt ttcctggcga tgaaaagttg gcattctgca tgggaccaga
     8581 agggattctt tttcttaaaa aaaccattca cctcatacca aaaaggagca acccaaaagg
     8641 gattgtcatg atagacctga aaaggaacct gataaaatat cttaaaatct tttttgtgtg
     8701 tcacgcgttg aactcggatg tcatgagaat aacttctttc gaaatcagca tctgactgga
     8761 atacatatct tacccgagcg tctgatggtg agttggaaat attgctacct ccatgatggt
     8821 ctcaacatag gtcggaccaa ttattcatgt tttcttaata aacattcttc ccaaatctat
     8881 cacaaattca gcattgatgt tgtaccgatg agaatgataa aagaaaaatc catgctttgg
     8941 gatatcatca tacctccttt ttctctgaag gaggcgctcc ttaggatatg aaattattta
     9001 cgactgtctc caaaatagga tggtgatgta aaaagtaaga caggagctga tggaggaacg
     9061 tcatccacgg aggaacgatt ttcactggtg ttgtgagagt caccgggatc gtatcaaaat
     9121 cggtagggtc attgatattt tccactcgaa ggaatccttg gaaggtttgc tctgattcat
     9181 taggtgcgac gactgagaca tgaactgtta ctgatccatc ctccggggtt aatcgaaccc
     9241 ccgattcagg gttaaaggac catgtccccc aagaaattga tgaggtgttc accgtccagt
     9301 tcaataagga gttgctatcc ccgatattcc gtacatcaaa gcttccgaca acggtcgcac
     9361 caggatcgat atccgtccat gaaagacttc cagagcagtc taaatcaggg gttgtcccat
     9421 tctctggtgg ctcaggctcc catggaggaa tatagacgac tttgaatacc tgacctccag
     9481 tgttatattg ccacaccgta gatccttcag gggtgacttc aaagaccaac cctgattctc
     9541 cattcgtgat aagggtgttt ccactaggga gccgccaagc gcctgagata tggcttgcat
     9601 aaaaactcgt gggaggattc gctgtataga tccatgtttg cgctgctggt ccgtatgatg
     9661 atccgggttc tagataatac tcgccgttct cgtcaaccgg tgtagtgatt tcatcaattg
     9721 tggagtaatg attgccaggg cggttcgctc cgttgttaaa gacaaggatg tctccttcgc
     9781 cagggcatcc ttcatcaatc catgacgcat catgttgact gaacaatttc ctgtcgtttg
     9841 tcgtacctgc atcataggtc attggatttc cccagcggta gagaagatcg ccgccttttc
     9901 cactatttcc accagtatga cccgctgctt cctctgtcgt ggtgctgtgg tcgatgaccc
     9961 agatttcatt gaaattatgg atacttatga gaatttggtc aaaatctacg ttataatcaa
    10021 ttgaattcgt atgcatccaa tcatattggg tcgaggtcgc ataattgata tcaactaatt
    10081 cagggtgatc tcctaccaca ccataatt
//
LOCUS       DATMXJ010000099         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_2135, whole genome shotgun sequence.
ACCESSION   DATMXJ010000099 DATMXJ010000000
VERSION     DATMXJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="hisS"
                     /locus_tag="VN377_02775"
     CDS             complement(<1..888)
                     /gene="hisS"
                     /locus_tag="VN377_02775"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWR27343.1"
                     /translation="MFQVPRGTRDFTPDEMQKRRYLEQNMTATFTSFGYGEIQTPTFE
                     NLELFTAKSGESIINELYAFTDKGGRTLALRPELTAPVIRCYVERLQMEPKPLKLFYF
                     GNCYRYDRPQKGRYREFQQAGCEIIGTDTIEATAELLALAYTLLKSTGLKNIRLNIGN
                     LTIISAIFKKLRLLKDDQKLLLPLIDKSQFDDLKIALRDCNVSEEMIDQLLEILQTSD
                     INKIRSFISDDPEATNELTAMEAILDLLKNAFHIDEYIIKLSIVRGLDYYKGLVFEID
                     APVLGAEKQLCGGGSYDLVP"
     gene            1096..1737
                     /locus_tag="VN377_02780"
     CDS             1096..1737
                     /locus_tag="VN377_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HWR27344.1"
                     /translation="MEINMYVNKYNNARYAFFKWRYQLELYNKVILAVSFACITALLA
                     QLRFYIPGSPVPLTGQTFAVVLSAVVLGKWWGGISQSIYLGVGMVGVPWFAGMNGGFA
                     YLAGPTGGYLIGFVLAAFFLGYCVDTYVRSRKYWTMFALMLFANFGIVFGFGLIQLYG
                     WLSLTGASVDLWGLLLMGLIPFIIGDTIKIAVAAGIASGITPKQAFGKEVDVN"
     gene            complement(1765..1944)
                     /locus_tag="VN377_02785"
     CDS             complement(1765..1944)
                     /locus_tag="VN377_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR27345.1"
                     /translation="MKIDLDTVKCIKETAITIRGSRRRITVPCEVVDILKLKDGDKLK
                     WIVLNDRTITLSKVK"
     gene            complement(1984..2751)
                     /locus_tag="VN377_02790"
     CDS             complement(1984..2751)
                     /locus_tag="VN377_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWR27346.1"
                     /translation="MNKSNSIQIETKALNVTIHSNPVLKNIDIQIPAKQLTAIIGPSG
                     CGKTTLLKSFNRLLEIQDGVTIHGEIVVDGINIMSSGLNVPDIRKKMGLISQKPYPLP
                     MSIFENVAYGLRIHHDKSAKELAEAVQNCLQEVGLWNEVKDRLHDPASRLSVGQQQRL
                     CLARAIAVQPEVILCDEPTSALDPIAAQQIEKLLLKLKKDFTVILVTHTLRQAKRVAD
                     YVIFLYFGKVIEQGPAAEFFNNPKNPETKAYLAGEIS"
     gene            complement(2767..3618)
                     /gene="pstA"
                     /locus_tag="VN377_02795"
     CDS             complement(2767..3618)
                     /gene="pstA"
                     /locus_tag="VN377_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWR27347.1"
                     /translation="MNRRNIEEKIFLGLMLASVFIVMGSLVLIVSVIVLNGAPSLSIE
                     ILTQTSEGGFYLGAGGGFLNAIVGSLLLALPATGLAFLISIAIALYLQKDFLNPKVAN
                     FIRICLDILWGVPSVVYGVFCLTIMIAIGMTGGSLFFGIIALTLLEIPIITRSIDESI
                     KMVPIGLKESAYSLGSSKFETAMKVVRRQAMPGVIAGVLLGFGRGIGDAASILLTVGD
                     SNYFPSSISDQTAALPTMILNLVSMPDQVVQNKAYAVAFVLLMIVLLISIITRYLTKK
                     SSKHIIK"
     gene            complement(3615..4469)
                     /gene="pstC"
                     /locus_tag="VN377_02800"
     CDS             complement(3615..4469)
                     /gene="pstC"
                     /locus_tag="VN377_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWR27348.1"
                     /translation="MLIVTASVIFLFFLLVAVLINGSLNILAEESLSSLLLSSTWKPF
                     SGEFGLFAVIIDTLIVTAISMFIAVPISLLSAIYIAEYAPKKFRNIVRPFIDVLAGVP
                     SVVFGLCALLVFVPLIRDDVAPFFGSNISSGFSLFTASLILSIMVFPIIISLCLESFN
                     SVPVALKEASLSLGATKWETIKKVIFRASGPGVLAAIFLGFGRAFGETIAVSMVIGNQ
                     ELIPKSLFGPGQTLASLIASNYTEVSSIPILASALIFVALILFVVVLIFNILGYLVLR
                     RASKRWKY"
     gene            complement(4737..5720)
                     /locus_tag="VN377_02805"
     CDS             complement(4737..5720)
                     /locus_tag="VN377_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWR27349.1"
                     /translation="MGEKTQKKMKILVTVLIIGVVGMVSLSGCTTTQKENTIKVSGAY
                     SLYPMMVIWAQEYHTLHPDINIDVSSGGAGKGMADALAGSVNLGMVSRDVTTAESDQG
                     IIAVAVVEDAVLATINTDNPVLDIILEKGLTKQQLREIFINYTIPTWGQLIGDSNNTD
                     PIKVYSRSDPCGAAEAWVKYLGDYTQDDIPTIEQITKVKGDDSMSKSIASDPLGIGYS
                     NVNYIYSNSTKAPKEGIAVVPLDLNENGTIDPEENFYESRDDIVNAEINNTLPSPPSR
                     LVYLVTLNSFTGITKDFVRWILTDGQQYTFDNGYGAVPNDILDDQLQILEG"
BASE COUNT         1614 a         1150 c         1335 g         1817 t
ORIGIN      
        1 ggggaccaga tcataggagc ctcctccgca gagttgtttt tctgccccaa ggacaggagc
       61 gtcgatctca aaaactaatc ctttgtagta gtcaaggcct cgtacgatgc ttaattttat
      121 gatatattca tcgatgtgaa acgcgttttt aaggagatca aggattgcct ccatggcagt
      181 aagttcattt gtcgcctcag gatcgtcaga gataaatgaa cggattttat tgatatccga
      241 tgtttggagg atttcaagga gctggtctat catctcttcg gaaacgttgc agtcacggag
      301 agctattttt aaatcatcga attgggattt atcaatcaat ggtaagagta atttttggtc
      361 gtctttgagg agacgtaatt ttttgaaaat agcactgatt atggtgaggt tcccaatgtt
      421 gagtcgtata ttttttaacc ctgtgctttt tagtaatgta taggcaaggg cgaggagttc
      481 tgccgttgct tcaatggtat ctgttccgat gatttcgcaa cctgcttgtt ggaactcccg
      541 atatcttccc ttctgcggtc ggtcgtaccg gtagcaattc ccaaaataga agagtttgag
      601 tggttttggc tccatctgta atcgttcgac gtaacaccgg attacagggg cagtaagctc
      661 aggtcgtaat gctaatgtgc gtcctccttt atcggtaaac gcgtagagtt cgttgatgat
      721 ggattccccg gatttcgcgg tgaagagttc caagttctcg aaggttggtg tttggatttc
      781 accatatcca aatgaggtga atgtcgcagt catgttctgt tctaaataac gccgtttctg
      841 catttcatcg ggagtgaaat ctctggttcc tcgaggcact tgaaacatag gcagtcgtat
      901 agtaggaatt ctttaattat gtttctctta agaatttagg gagatcgggt cttattgaag
      961 agtgatttat gtagaggttt aatctcctag tatcttccct ttattctctg aagaatgtag
     1021 aaaaaagagg caaaaacttc ttttagcagt atccgttaca cctcttgaat ttcttcctca
     1081 gagaggttga tttatatgga aataaacatg tatgtgaata aatacaataa tgctcgatat
     1141 gcgtttttta aatggcgtta ccagcttgaa ctctataata aagtaattct cgcagtaagt
     1201 tttgcgtgca taacggcttt gttggctcag ttgcgatttt atatcccggg atcccctgtt
     1261 cctctgacag ggcaaacgtt tgctgtcgtt ctgagtgcag tggtacttgg aaaatggtgg
     1321 ggtgggataa gtcagagcat ctatcttggt gtaggtatgg taggagtccc ctggttcgca
     1381 gggatgaacg gaggatttgc ctatcttgct ggacctaccg gtggttacct tatcgggttt
     1441 gttcttgctg cgtttttcct tggatactgt gttgatacct atgtccggtc acggaaatat
     1501 tggacgatgt tcgcattgat gctctttgcg aattttggaa tcgttttcgg ctttggattg
     1561 attcaactgt atggctggct ttctttgact ggagcgtcgg ttgacctgtg ggggttgctc
     1621 ctcatgggtt tgatcccatt catcatcggt gatacgataa agatagcggt tgctgctggc
     1681 atcgcgagtg gtataacacc gaaacaagca tttggaaaag aagtcgatgt gaactaatca
     1741 attcgatagt tccattcttt ttttttattt tacttttgaa agagtgatcg tacgatcatt
     1801 caggacaatc cacttgagtt tatctccatc ttttagttta agaatgtcga ctacctcgca
     1861 aggaaccgtg attcgcctgc gtgaaccacg gatggttatc gcggtttctt ttatacattt
     1921 cacagtgtca agatcaattt tcattttatc tcactctctt cgttctatca aatggtagga
     1981 tagctaactt atttctccag ctaaataggc ttttgtctca gggttttttg gattgttgaa
     2041 aaattcagca gcaggtcctt gttcgatgac cttgccgaaa tacaggaaga tgacgtaatc
     2101 agccactcgt tttgcttgac gcaaggtatg ggtgacaagg ataaccgtga agtctttctt
     2161 taatttcaac aggagttttt caatttgttg ggctgcgatc gggtcaaggg cagaggttgg
     2221 ttcatcacat aagataactt ctggttgtac tgcgatggct cgggcgagac ataatcgttg
     2281 ttgctgtccg accgataatc gtgaggcagg atcatgcaac cggtctttaa cttcattcca
     2341 gagaccgact tcctgtaaac aattttgtac tgcttctgcg agttcctttg cacttttatc
     2401 atggtgaatg cgtaatccat aggcgacatt ttcgaatatc gacatcggta atggataggg
     2461 tttctgtgag atcagaccca tttttttcct gatatctggg acattcagcc cgcttgacat
     2521 gatgttaatc ccatcgacga caatttcacc gtgtattgtt acaccatctt gaatttccag
     2581 caacctgtta aatgatttca acaaggtggt tttcccacat ccggatggac cgatgatagc
     2641 agtgagttgc ttggccggta tctgaatgtc aatattcttt aacacaggat tactatgtat
     2701 ggtgacattc agggccttgg tctctatctg aatactattg gacttattca ttcatgattc
     2761 ctctttttat tttatgatat gtttcgatga tttttttgtt aggtatctcg ttatgatact
     2821 aatcaataac acgatcatga gtaaaacaaa ggctactgca tatgctttgt tttgaacgac
     2881 ttggtctggc atactgacga gattgaggat catcgttggt aacgccgctg tctgatcgga
     2941 gatcgaggag ggaaaataat tcgagtcacc cacagtgagt aatatcgatg cagcatcacc
     3001 aatccccctc ccgaacccaa ggagaacgcc agcaatgacc ccgggcatcg cttgacgccg
     3061 aaccaccttc attgccgtct caaattttga agatcccagg gaataggcac tttctttcaa
     3121 tccgatcgga accatcttta tggattcatc gatgcttcga gtgataatgg ggatttcaag
     3181 caatgtgagg gcgattatcc caaagaaaag cgagcctccg gtcatcccaa tcgcaatcat
     3241 gatggttaag caaaacacac catacacgac tgaagggact ccccagagaa tatcaaggca
     3301 tatgcgaata aagtttgcca ccttggggtt caaaaaatct ttttgaagat acaaggcaat
     3361 tgcgatgctt attaaaaagg ctagtcccgt ggcaggcaat gcgaggagta aagatccaac
     3421 gatagcattg agaaacccac caccggctcc aaggtaaaac cctccctcgg aggtctgggt
     3481 gagtatttca atagaaagcg atggggctcc attcagcaca ataacggaaa caattagtac
     3541 aagactgccc atgacaatga atacagaagc gagcattagt ccaagaaata ttttctcttc
     3601 gatgtttcgt ctattcaata cttccacctc ttacttgctc ttcgcaggac cagatatccg
     3661 agaatattga aaatgagcac gacaacaaaa agtattaagg cgacgaagat gagcgctgaa
     3721 gcaagaatcg gtatggagct tacctcagta tagttcgaag cgatgagtga agcaagtgtc
     3781 tgaccaggtc caaagagaga ttttgggatg agttcttggt ttccgattac catgctgacc
     3841 gcgattgtct caccaaatgc tctgccaaaa ccgaggaata tcgcagcaag caccccggga
     3901 ccagaggcac gaaagatgac cttttttatg gtctcccatt tcgtggctcc caaagaaagt
     3961 gatgcttctt tcaaagcgac aggcacagaa ttgaacgatt cgaggcatag tgatattatt
     4021 atagggaaga ccataattga taaaatcaac gatgcagtga acaaactgaa tccagagctg
     4081 atattgctgc caaaaaaagg tgcaacatca tctcgtatta agggaacgaa cacaaggagg
     4141 gcgcagagcc caaaaacaac agatggtaca ccggcgagta catcaatgaa tggtctgacg
     4201 atattcctaa atttttttgg agcgtattca gcgatataaa ttgcgctaag taaagaaatt
     4261 ggtactgcga tgaacatgga gattgcggtg acgatgaggg tgtctatgat cacagcaaaa
     4321 agaccaaatt caccggagaa cggtttccat gttgaggata agaggaggct ggaaagggat
     4381 tcctctgcga gtatatttag agaaccgttt atgaggactg ctacgaggag gaaaaataaa
     4441 aaaatgacgg atgctgtgac gataagcatc agtcgggttc ctaaggtgtc atagagtttt
     4501 ctggtaatct taatgaaatt atatatcaat agaataatga agattatgag aaataatgca
     4561 aaaaggatga aattgttgag aagaaataaa aaaataaatg aaacaaaaag ggataatatg
     4621 ccgactagtg ctaatttatt ttttaataca ctgcttttag gactgggagg catattcatc
     4681 ctgtattggt ttatgatttc cagggaggaa cagtgatttg gttttatcct ccttgtttag
     4741 ccttcgagta tctgcaattg gtcatcgaga atatcatttg ggacggcacc atatccgtta
     4801 tcgaaggtgt attgctgtcc gtcggttaag atccatctga cgaaatcttt ggtgattcct
     4861 gtgaagctat tcaaggtgac cagatacacc aaccgtgatg gtggggaggg taacgtgtta
     4921 tttatctctg cattgacgat gtcatccctg ctttcgtaaa aattctcttc agggtcaatt
     4981 gtaccgttct cattgaggtc gagaggaacg accgctattc cttctttggg tgctttcgtt
     5041 gagtttgaat agatatagtt cacattgctg tatccgattc caagagggtc acttgcaata
     5101 gattttgaca tcgagtcatc gccttttact tttgtgattt gttcgattgt cggaatatca
     5161 tcttgtgtat agtcaccaag gtattttacc caggcttctg cagcgccaca gggatcagat
     5221 cttgaataga ccttaatagg atcggtattg ttcgaatccc cgatcaattg tccccacgtc
     5281 ggtatggtat aattgataaa aatttctcgt aattgttgtt ttgttaaacc tttttctaat
     5341 attatgtcta acacagggtt gtcagtgttt attgttgcaa ggacagcatc ttcaacgaca
     5401 gcgactgcaa tgatgccttg gtctgattca gcagtggtaa catctcttga aaccattcca
     5461 aggtttacgg aacctgcgag ggcgtctgcc atcccttttc ctgctccacc tgatgaaaca
     5521 tcaatattta tatctggatg gagtgtgtga tattcttgtg cccatatcac catcatagga
     5581 taaagagagt atgctccaga tacttttatc gtattttcct tttgcgtggt ggtgcatcca
     5641 gaaaggctca ccattccgac cacacctata ataagaactg ttacaagtat tttcattttc
     5701 ttttgtgttt tttctcccat ttttcaacgt ctccattttt ttctgaacgg ttgctatgaa
     5761 tgatgatagc aagcaacgta tcagcaataa tattatacac agccccctat ttataattat
     5821 tggtaaattt ccaacatcgc accaatgtga aaaataaatg aatgttgaaa ctcctcaatt
     5881 gaaaaatgca ttatatctta tgtactatta gtatag
//
LOCUS       DATMXJ010000100         9893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_75329, whole genome shotgun sequence.
ACCESSION   DATMXJ010000100 DATMXJ010000000
VERSION     DATMXJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75329"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1143
                     /locus_tag="VN377_02810"
     CDS             136..1143
                     /locus_tag="VN377_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
                     /protein_id="HWR27350.1"
                     /translation="MYIYDGKLYLTKDHVHLLKKEPEYVKQRYPAVSNASFSKSNIIR
                     DKMAHDLGLNIHHFNRLLKKYRDEGLSGLRNSSTRTHQSPNHTEPWLEELVIKVREKT
                     GFGSAHLSLIVNISLENQGRHEWVHPRTISRILVRRGIIESEKRAKTEWKRFEWGHPN
                     RLVQSDLTLFNGIPLLTMEDDYSRRGWAIRLKDQRDATVINGMKTLLKIKYDNLLTDN
                     GSQFSRRNKVIREYCEHYINEKHIWTSIHHPQTMGKLSAFQKGLKRFLRHKLGTSQDI
                     HEIDLYIDIYVPWYNNGKYHSTISSYPEERYSGQRDIEWYNSLVKSLKLEDVLVIKSE
                     G"
     gene            1147..1359
                     /locus_tag="VN377_02815"
     CDS             1147..1359
                     /locus_tag="VN377_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR27351.1"
                     /translation="MSCPYTYGRGSVDAKSSLATMICAAQNFIHNAMKRIVVIGAVDE
                     EGNSSGARNLLGKYNPMCIVIGEPSE"
     gene            complement(1617..4829)
                     /locus_tag="VN377_02820"
     CDS             complement(1617..4829)
                     /locus_tag="VN377_02820"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27352.1"
                     /translation="MAQSDIISPRNANEEVLRYHTGTIDSAFGGSGGGTLEYAIRFTP
                     TELVQWSGWSIQAVDFYHWEPDENFGEIKIYDEGTSTTPGTLISSEPYTLNEVGWKRI
                     NLTNPVLLNTSRDIWVSTEITFQPGYPMGLDDTFHVAGKSAWYKPPGGLFTDVGNYYS
                     WSLEAVIVQTFQWDHDISILNINTPESGTGHIVTPEITIRNRGLTDEYAVPINLKVSY
                     WNTSLNNWVIEYNQTLDIDINSNQTNDVVFPDWTPLVLGTENVDVDYLVQASVQITND
                     MDPNNDYLSKEVSLHFDYLHNLGVNEIVSPNSGRPAVISPELIMENYGQNDETDVNVS
                     FTIQRLSNITTILLEEDFENGIPSTWTVTDGYNDGATWRDDNPWGRIPPPGSNCDGTF
                     VICDELYEETPMNEYLASPVFSCVGETTILFDFGSYFRSIPQYDQYGEVDAFNGTTWT
                     TVYYVTDTSAFAQKYIDLSPLLAGVPIAQIRFHFFSNDWAYFWMVDNIKIYGATFVTE
                     YQESHYLDIESAEIISVTFSDWTPWTPPTFSTKVEYKITGGTQLDQGVLDGDPIDNNL
                     VKWITVEHIHDVGVERITKPSRSPIEWLYYHYGYANNGVGLTSGGTYEAAMRLTPEEL
                     GEYNNRKIDIVKFYHHEAGTHVGNIKIYTEGTSESPGPLITSEPYTVTGKGWYEVILS
                     SPVTINAEEDLWVSVEITHSAGQYPIGIDSGPAVLGKGDWIYINGGWEEISGAGLNSN
                     WCIEAHLKDHIEWLPGTYPVEAIVANYGTYNEITFNVNASIYKETERGDELFYHNDSI
                     FTQTLEPGDNATVQFADVTFTDSDEGTYWLEITSELPSDDNTGNNQEEMEFIISVPDT
                     MPPETSHEFSGTIGDNNWYISDVTLLLTATDPTPPYKRFNSGKGPSGVNHTYYKIDAG
                     AWLEYTTPVVVSVDGQHQVSYYSIDMVGNTEPTQGPFSFKMDKTSPQFTGYTFTPLNL
                     LKNKWLCVADVTDATSGVVRVEFYVDDALVGSVTTAPYEFEFDGNPTNNSKALAYDAA
                     GNSALSPIAQYIEYDYQAQSYPTIQLLQEVNLRTTTQRSE"
     gene            complement(5077..9699)
                     /locus_tag="VN377_02825"
     CDS             complement(5077..9699)
                     /locus_tag="VN377_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27353.1"
                     /translation="MMKKTKNTYKAVVAFAVAMAFVMPGAAVGSYLENTMNQDSTSLS
                     VDDAIIDLQSFEKDAISRNTKSADVPYSTAITIPEDPTSDEQDYWVSPEQARASRNIV
                     LREYGISEEKILTMTDEEAMNLRNSLTTTQERTGGMIMGTLDPVDWWPEENHDAANTA
                     YTTATAPDLYNATLLWKKNLGGRTEGSTPVVDGKIITASKDKPPIYCLNASTGEEIWN
                     YSAALSSSPAIVGGRVYATVGSRIVCLNFSTGALIWMNSESHGWTPPAVVGNRIYYGG
                     YNNNYADTFYCIRTTDGTEIWNVTLSDVQYSNTMNYGSPAIANDKVYFGTGRIGLGPH
                     KLYCYDINGNHIWNVTTPGEAADTSPTVVNGKVYISNGDLSGSHTLCYNATTGNLVWS
                     HSAEDAFGYRGVAVANGLVYAKELLTLYCLYAENGTEKWSYNYQEYGGCNGAGPSVTA
                     DGKVYVQKDSYPVGTPNLICLDANTGAYLGGFLADIGEIRGTPVVANGKIYIQGRDNN
                     HIYCLGEVDHDIIPLNIDSPVSGPEPGTFTPEVTIRNQGRSNETDVPVTMKITNIYRY
                     LYEDFDSVIWPPAGWTEEQPNEWRKSQSNNAGGQPPEEFLLPIIYLIGTTAWIQSPAR
                     NTSAATELTLEFKCKLQGFVPNQASAYVYVRANASQPWTNVAPWPNPLNGNTYGAAMF
                     QINITPYIGTGTQVRFEYTGAKQGSTGWYIDDVSIMTPGETPEYNETVNVDILSKETM
                     TTTFPEWTPTSLYYIVDVDTDLQTDEVTWNDMITEVVTFHYYDDLAVLGINAPLSGPA
                     SVITPEVQVGNFGSFNMTNQSVNLKIGEPQPTFLEEDFSNLPFPPTGWTVEEPNEWQQ
                     NPSNSAGGKAPEARLSSSVTGNYASLQSPPVDTTAVSELTLKFKSYIYNKYGGYNCSI
                     WVRKDTMHLWVEVTPWSNPIAGNVPPTAYNIDITPYIGTGTQVRFEFDGNASCLRYWY
                     LDDVVILKVIPLEYDETVLINITADNVMTVVFPDWMPEYFNYDYSGSIDYEVTAYTSV
                     AGDEFPENDQVVNLITLDFLHDAAVKNIAEPVWEYFHNGYANNGVGLTSGGTYEAAMR
                     LTPSELQNYDGGKINAVKFYHHETGSHSGQIKIYAEGTSGSAGSLITSEPYTVTGKGW
                     YEVTLSSPVTINAEEDLWVSVEITHSAGQYPIGIDNGPAVDGKGDWIYINGGWEEIQY
                     GGLDKNWCIESQLEKNVEWEPGTYPIDAVITNYGTYDESNFNVNAKIWLILDKDWILY
                     YESNHMVTTIMQPGDELNVVFNDVVFGSDDIGDYRLEITTELPGDDDNTNDQCVLLFK
                     VVLIDHLPPETTHEFSGTIGDNNWYISDVTLVLTASDPTPPYKRFNSGKGPSGVNHTY
                     YKIDSGAWTEYTTPVIVSTDGQHQVSYYSVDMAGNTEPTKGPFNFKMDKTSPQFTGYT
                     FTPLNLLKNKWLCVADVTDATSGVVRVEFYVDDALVGNVTTTPYEFEFDGNPTNKSQA
                     LAYDAAGNSALSPIAQYVELDYQVKSDPTMQFLPGVTLRTTAQRSE"
BASE COUNT         2698 a         2151 c         2058 g         2986 t
ORIGIN      
        1 ctgcacataa atccccctat ttgtgtgcaa agcttatata tatacttttt accaatatta
       61 aattggtgaa aaatgtgtta ggacaggaca tatgtccctt ctggtgatac ccttaagcaa
      121 caaggggctg gacatatgta catttacgat ggcaaactct acctcacaaa agatcacgta
      181 cacctcctga aaaaggaacc agaatacgta aagcaacgat accctgcggt ctcaaatgcc
      241 tcgttctcca aaagcaacat catccgagac aagatggctc atgatctagg tctcaacata
      301 caccacttca accgcctttt aaaaaagtac cgagatgaag ggttatctgg tttacggaac
      361 agttcgacac gaacacatca aagcccgaac cacacagaac catggctgga ggagcttgtc
      421 attaaagtac gggaaaaaac aggattcggc tccgctcatc tctcgctgat tgtgaacatc
      481 agtctagaga accaggggag acatgaatgg gtgcaccctc gcacgatctc caggatcctg
      541 gtccgacgag gtatcatcga atctgagaaa cgtgcaaaaa ccgaatggaa acggttcgaa
      601 tggggccatc ccaaccgact cgttcaatcc gatctcaccc tgtttaacgg catcccactc
      661 ttaaccatgg aagatgatta ctcccgtcga ggatgggcaa tccgtctgaa agaccaacga
      721 gacgccaccg tcattaacgg catgaaaaca ctcctgaaaa tcaagtacga caacctactg
      781 accgataacg gatcacagtt cagcaggagg aacaaggtca tccgtgaata ctgtgaacac
      841 tatatcaacg aaaaacatat ttggacgagc attcaccatc ctcagaccat gggaaaactc
      901 agcgcattcc agaaaggatt gaaacgattc ctccgccaca aacttggcac ctcacaggac
      961 atccatgaga tcgatctgta tatcgacatc tacgtccctt ggtataacaa cgggaaatac
     1021 cattcaacaa tctcctcata tcctgaagaa agatattcag gacaacgaga tatcgaatgg
     1081 tataacagcc ttgtcaaaag ccttaaatta gaggatgttc ttgtcataaa atccgagggg
     1141 tgacatatct catgtcccta cacatatggg cggggaagcg tagatgcgaa aagttcatta
     1201 gcaacaatga tttgtgctgc tcagaatttc attcataacg caatgaaaag aatcgtggtt
     1261 attggtgcag ttgatgaaga aggaaattca tcaggtgctc gaaatctttt aggaaaatat
     1321 aaccccatgt gtattgttat cggtgaacca agtgaatagg atgctattac cattttgcaa
     1381 caagccttat aataaacagg attctaatca gtagcggatt ctgatgtaga tctgctcttg
     1441 aacggaagca gatgaagtgg ggagcaagtc acgatcaggt taagaaatct agttgagcaa
     1501 tgatttgata tcttgaagta tcgtttcaaa cgatttaatc aaaatgggct taatattaca
     1561 gaacttagga taaatgaagc tgcatgaaaa caaaaaaaga gagagggagt gaaggattat
     1621 tcactccgtt gtgtggttgt ccttagattc acttcttgaa ggagctgtat ggttggatag
     1681 ctctgtgcct ggtagtcata ttctatgtat tgcgcgatgg ggctgagtgc tgagttgcct
     1741 gcggcatcgt aggcgagcgc tttggaattg ttggtgggat tgccgtcaaa ctcgaactcg
     1801 tagggcgctg tagtgacact cccgactaag gcgtcatcaa catagaactc tacccggacg
     1861 actccgctgg ttgcatcggt gacatccgcc acacataacc atttgttttt caacagatta
     1921 aggggcgtga atgtgtagcc agtgaattgt ggtgaggttt tgtccatctt gaagctgaat
     1981 ggaccttgag tcggttctgt attccccacc atgtcgatcg agtagtacga tacttggtgt
     2041 tgaccgtcta ctgaaacaac gacaggtgtt gtgtactcga gccaagcacc agcgtcgatc
     2101 ttgtaatacg tgtggttgac accagacggt cctttaccgc tgttgaatcg tttgtagggt
     2161 ggtgttggat ctgtcgcagt gagtaatagg gtaacatcgc tgatatacca gttgttatca
     2221 cctattgttc cgctgaactc atgggatgtc tcagggggca tggtgtcagg gacgctgatt
     2281 ataaactcca tttcctcttg attgttacct gtgttgtcat cacttgggag ttccgaggta
     2341 atttctagcc aatatgttcc ttcatcagag tcagtaaaag tcacatcagc aaactgaacg
     2401 gtcgcattat cacccggttc gagagtttgc gtgaagatcg aatcattgtg atagaacaac
     2461 tcatcacctc tttcggtttc cttataaatt gatgcgttga cgttaaaggt aatttcattg
     2521 taggtaccgt agttagcaac aatggcttcc acaggataag taccaggtaa ccattcgata
     2581 tgatctttga gatgggcttc gatacaccag ttactattga gaccagcccc tgatatctct
     2641 tcccaaccgc catttatata gatccaatca ccttttccaa gaacagctgg gccactatca
     2701 ataccgatcg gatattggcc tgcggaatgt gttatctcga cggataccca gaggtcctcc
     2761 tctgcattaa tggtaacggg gctagatagt ataacctcat accatccttt tccagttaca
     2821 gtgtatggct cagaggtgat gagtggacca ggagactcag atgtaccttc tgtatatatt
     2881 ttgatattac caacatgtgt acctgcttca tgatgataaa attttacgat atcgatcttt
     2941 ctgttattat attctccaag ttcttctggt gttagcctca ttgctgcttc ataagtaccg
     3001 cctgaagtaa gacctactcc attattagcg tatccataat ggtaatataa ccattcaatg
     3061 ggtgatcttg agggcttagt gatacgttca acaccgacat catgaatatg ctccactgta
     3121 atccatttaa cgagattatt atctattggg tcgccatcaa gcactccttg atctaactgc
     3181 gttcccccag tgatcttata ctcaactttt gtggaaaagg taggaggtgt ccagggtgtc
     3241 caatctgaga aggtcacaga tattatttct gctgattcaa tatcgagata atgagattcc
     3301 tggtattccg tgacaaatgt agctccgtaa atttttatgt tatcgaccat ccagaaatac
     3361 gcccagtcat tggaaaaaaa atgaaatcgt atttgcgcga ttggtacacc tgccaaaagt
     3421 ggggatagat caatgtattt ttgtgcaaat gcagaagtat cggttacata atagacggtt
     3481 gtccatgtag ttccattaaa tgcatctacc tctccatatt gatcatattg tggtatgctc
     3541 ctgaaataac taccaaaatc gaataaaatc gttgtttcgc cgacacagga aaacaccggt
     3601 gaggcgaggt actcattcat cggtgtttct tcatagagtt catcacaaat aacaaaggtt
     3661 ccatcgcagt tactacccgg tggaggaatt cgaccccaag gattatcatc cctccaagtt
     3721 gcaccatcat tatacccatc agttacagtc caggtactcg ggataccatt ttcaaaatct
     3781 tcttcaagga gtattgttgt gatgttgctt aatcgttgaa tagtgaaact cacattgaca
     3841 tccgtttcat cgttttgccc atagttctcc ataatcaact caggagaaat aactgctggt
     3901 cttccactat ttggtgatac tatttcattt accccaagat tatgaagata atcaaaatgc
     3961 agcgatactt cctttgacag ataatcattg tttggatcca tatcgtttgt tatttgaaca
     4021 gatgcttgaa ccaaataatc aacatcaacg ttttctgtac ccaaaaccaa gggtgtccaa
     4081 tcaggaaata ccacatcatt ggtttgatta gaattgatat caatatccaa cgtctggtta
     4141 tattcaatga cccaattatt cagtgatgtg ttccagtaac tcacctttag attaatcggc
     4201 actgcatact catcggttaa tcccctgttt cgtatggtaa tctctggtgt aactatatgt
     4261 cccgtaccac tttcaggcgt gttaatattt aatatgctga tatcgtgatc ccattgaaat
     4321 gtttgaacga ttactgcttc tagcgaccaa ctataataat tgcccacatc agtaaacagt
     4381 ccgcctgggg gtttatacca agcactttta ccagcgacat gaaaggtatc atccaaaccc
     4441 attggatatc ctggttggaa tgttatctcg gtggacaccc atatatctct ggatgtatta
     4501 agtaacacag ggttagtcag gtttatccgt ttccatccca cttcatttaa tgtgtatggt
     4561 tctgatgaga ttaaggtacc tggagtggtt gacgttcctt catcgtatat tttaatttca
     4621 ccaaaatttt catcaggttc ccaatggtaa aaatcaactg cttgtatgct ccatccactc
     4681 cactgaacca gttctgtagg ggtgaaacga atagcgtact ccaatgttcc tccaccacta
     4741 cctccaaagg cgctatcgat agtaccagta tgatagcgaa gtacttcttc atttgcattt
     4801 cgtggtgata ttatatcact ttgtgccatt cggaaggtat cattaggtac ataggtatat
     4861 atttcttcag aaatttctct ttcaattttt attgtatctg gtataggtgt aagaaggttc
     4921 gatgatgcta ttacatttat aatcatagaa gaaataagaa tatatacaat aaccatcgtt
     4981 atttttattg actttttttt catcattgtt ttcacctttt ttaatacatt tctatctata
     5041 tttatcttaa aaaaagaaga gagggggagt gaaatattat tcactccgtt gtgcggttgt
     5101 ccttagagtc actcctggga ggaactgcat ggttggatcg ctctttacct ggtagtccaa
     5161 ttcgacgtat tgcgcgatag gactgagtgc tgagttgcct gcggcgtcgt aggcgagtgc
     5221 ttgggacttg ttggtgggat taccgtcaaa ctcgaactcg tagggtgttg tagtgacatt
     5281 cccgaccaag gcgtcatcaa catagaactc tacccggacg actccactag ttgcatcggt
     5341 gacatccgcc acgcataacc atttgttttt caacagatta aggggcgtga atgtgtagcc
     5401 agtgaattgt ggtgaggttt tgtccatttt gaagttgaac ggaccttttg tcggttctgt
     5461 gttccccgcc atgtcgacgg agtagtacga tacttggtgt tgaccgtctg ttgatacaat
     5521 gactggtgtt gtatactcgg tccacgctcc actatcgatc ttgtaatacg tgtggttgac
     5581 accagatggt cctttaccgc tgttgaatcg tttgtagggt ggtgttggat ctgatgcagt
     5641 gagcactagg gtaacatcgc tgatatacca gttgttatca cctattgttc cgctgaactc
     5701 atgggtcgtc tcagggggca gatgatcaat aagtaccact ttgaagagga gtacacattg
     5761 gtcattggtg ttgtcatcat ccccaggtaa ttcggtagtg atctcaagtc ggtagtcacc
     5821 aatatcatca ctgccgaaga caacatcatt aaacacgaca ttgagttcgt caccaggttg
     5881 catgattgta gtgaccatat gattactttc gtagtataaa atccagtcct tatcgagaat
     5941 taaccatatt ttggcgttga cattgaagtt gctttcgtcg taggttccgt aattagtgat
     6001 aaccgcgtca atgggatatg ttcctggttc ccattcaaca tttttctcga gctgggattc
     6061 gatgcaccag ttcttatcta aaccaccgta ttggatttct tcccaaccac cattgatata
     6121 aatccaatca ccctttccat ctacagcggg gccgttatca ataccaatag gatattggcc
     6181 tgcggaatgt gttatttcga cggataccca gaggtcttct tctgcattaa tggtaacggg
     6241 actagataat gtcacctcat accatccttt tccagttaca gtgtatggct cagaggtgat
     6301 tagtgaacca gccgaaccag atgttccctc agcatatatc tttatttgac cagagtgtga
     6361 acctgtctca tgatggtaga acttgactgc gttgatttta ccaccgtcat aattttgcag
     6421 ctcgctcggt gttaacctca ttgctgcttc atatgtacca cccgaggtaa gacctactcc
     6481 attattagca tatccgttat ggaagtactc ccaaactggt tctgctatgt ttttcacagc
     6541 tgcatcgtgg aggaagtcta aagtgatcaa attaacaact tgatcgttct cggggaattc
     6601 atcacccgca accgaggtat aggctgtcac ttcatagtcg atggaaccag aatagtcgta
     6661 attgaaatac tctggcatcc aatcaggaaa taccacggtc atgacattgt cagcggtgat
     6721 gtttatgagt actgtttcat cgtactccag agggattact ttcaggatga cgacatcatc
     6781 caggtaccaa tatctgaggc aactcgcatt tccgtcgaat tcgaatcgta cttgagtacc
     6841 ggttccaata tagggggtaa tgtcaatgtt atatgcagta ggcgggacat tgcccgcgat
     6901 aggattggac catggtgtga cttcaaccca aaggtgcatg gtatcttttc tcacccaaat
     6961 ggagcaatta tatccgccat atttgttgta gatgtaactc ttgaatttca acgtgagttc
     7021 tgagacggca gttgtatcca ctgggggtga ttgaagggat gcatagttgc cagtaacgct
     7081 actagataac ctggcttctg gtgcctttcc accggcgcta ttagatggat tttgttgcca
     7141 ctcatttggt tcttcgacag tccatcctgt tggcgggaac ggtaagtttg agaaatcttc
     7201 ctctaaaaac gtgggttgtg gttccccgat tttcagattt acagattgat tggtcatgtt
     7261 gaagcttccg aagttaccta cttggacttc tggtgtgatc acagatgcag ggccgcttaa
     7321 aggggcgtta ataccgagca cagcaagatc atcatagtag tggaatgtga cgacttctgt
     7381 tatcatatca ttccaggtga cctcatcagt ttgtaaatca gtgtctacat cgacgatata
     7441 atacaaactt gtgggcgtcc attcagggaa ggttgtggtc attgtctctt tcgataaaat
     7501 atcaacgttt acagtttcat tgtattctgg tgtctcccca ggagtcataa tcgatacatc
     7561 atcaatatac catccagttg aaccttgttt ggctccggta tattcaaagc gaacctgtgt
     7621 gcccgttcca atatacggag taatatttat ttggaacata gcagcgccat aggtgttccc
     7681 gttgagtgga ttcggccatg gtgcgacatt cgtccagggt tgagacgcat tagcacggac
     7741 atacacgtat gcagatgctt gattgggaac aaatccttga agtttacact tgaactccag
     7801 ggtgagttct gtcgccgctg aggtatttct tgcgggagat tgtatccatg cagtggttcc
     7861 gattaaatat ataatgggca ggaggaattc ctctggaggc tgcccacctg cattatttga
     7921 ttgagatttt cgccattcgt tcggttgttc ttcagtccat cctgcaggtg gccagataac
     7981 gctatcgaaa tcttcgtata aataacgata gatattagtg attttcattg tcacgggcac
     8041 atctgtctca ttacttcgcc cctgatttcg tatagtgact tcaggtgtga aagttcctgg
     8101 ttcaggacca cttaccggac tatcgatatt cagagggata atgtcatgat cgacttctcc
     8161 gagacaatag atatggttgt tgtcccttcc ttggatatat attttcccat tcgcaaccac
     8221 aggagtacca cggatctccc caatatccgc aagaaatcca ccgagatacg ctcctgtatt
     8281 cgcatctaaa catattagat taggagtacc taccggataa gaatcttttt gtacatatac
     8341 cttaccatct gcggtaacac tagggccagc tccattgcag ccaccatatt cttgataatt
     8401 atagctccat ttttcagttc cattttcggc atacaaacag taaagtgtaa gcaactcttt
     8461 tgcatatact aaaccattgg caactgctac tcctctataa ccaaaggcgt cttcagctga
     8521 gtgactccat actaaattac ctgtagtcgc gttgtaacat aaggtatgac ttccagacaa
     8581 atcaccgtta ctaatataga ccttaccatt aactaccgtt gggctagtat ctgctgcttc
     8641 accgggtgtg gttacattcc atatatggtt tccgttaata tcgtaacaat agagtttatg
     8701 gggacccaga cctattcgcc cagtaccaaa atagactttg tcgttggcaa tcgctggaga
     8761 cccataattc atagtattac tatattgaac atcactaagc gttacattcc atatctcagt
     8821 cccatcagta gttcttatac aatagaatgt atctgcatag ttattgttat atccaccata
     8881 atagattcta ttaccgacaa ctgcaggtgg tgtccatccg tgactttccg aattcatcca
     8941 tatgagagca cctgtactaa aatttaaaca aactatccta ctcccaacag tagcatagac
     9001 cctgccacca acaatcgcag gagaactaga taatgctgct gagtaattcc atatttcttc
     9061 tcctgtacta gcatttaaac aatatattgg tggtttatcc ttactagctg tgatgatttt
     9121 accatcaact acgggagttg atccctcagt cctacccccc agattctttt tccataacag
     9181 cgtagcgtta tagagatctg gtgcggtcgc agtggtatat gctgtgtttg ccgcgtcatg
     9241 gttttcttct ggccaccaat ccacggggtc caaggttccc ataatcatgc caccagtacg
     9301 ttcttgagtt gttgtcagtg aatttcgtag attcattgct tcttcatcag tcattgtcaa
     9361 tattttttct tcactaatgc catattcacg tagcactatg tttcgagatg ctcgtgcttg
     9421 ctcaggggat acccaataat cctgctcatc actagttgga tcctcaggga ttgttatcgc
     9481 agtactatag ggaacatctg ctgatttagt gttcctcgat atggcatcct tttcaaatga
     9541 ttgcagatcg atgatggcat cgtcgacact caaactcgtg ctatcctgat tcattgtgtt
     9601 ttccaagtaa gaacctaccg ccgcacccgg catcacaaaa gccattgcga cggcaaatgc
     9661 tacaactgcc ttatacgtgt ttttggtttt tttcatcatt ttgcttttct cctttttttt
     9721 gctataataa tatcttatac acaaatacaa atcgttgtag gagtcccgtc ctacccgttg
     9781 ttcgccttcc tctttatgat gatgcgtcgt aagacataaa aaataattat atattctatt
     9841 aataatatta taataatgat tatacaaact tatataaaga tcttggatat cta
//
LOCUS       DATMXJ010000101         9448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_44132, whole genome shotgun sequence.
ACCESSION   DATMXJ010000101 DATMXJ010000000
VERSION     DATMXJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44132"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1141)
                     /gene="fen"
                     /locus_tag="VN377_02830"
     CDS             complement(110..1141)
                     /gene="fen"
                     /locus_tag="VN377_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HWR27354.1"
                     /translation="MGVDLGDLFTKEPCAFEDFKDRVVALDGYNILHQFLSSIRQRDG
                     TPLKDSQGRITSHLSGLLYRTSNMVAAKIRPVYVFDGVPHPFKARTIQQRRERKEQAE
                     REWKEALEKGDLEKAKSKAQQTSRVTAEIIEQSKQLLDALGIPYIQAPSEGESQASYM
                     VRKGDAFAVGSQDFDCLLFGAPVLVRNLTSSEKRKLPNKQAYTAVHPERIRLEPGLQK
                     LGISREQLVDIAILIGTDFNDGIKGYGPKKSLKLLKEKGTIENAFGSIPEVERNFTYD
                     EIAIVRKIFLEPTITREYSLQWSKPDSSAVIRLLCDDHQFSRDRVEPILENFSSINQL
                     TKQKNLFDF"
     gene            1225..2145
                     /gene="argF"
                     /locus_tag="VN377_02835"
     CDS             1225..2145
                     /gene="argF"
                     /locus_tag="VN377_02835"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWR27355.1"
                     /translation="MKRDLISMLDVKDDLNDIIKLSIKLKQQSKKGKRIEYLKRKTLG
                     MIFEKPSTRTRVSFETGMTLLGGHAIFLSSNDIQLGRGESIQDTAQVLSRYVDMIMYR
                     AKSNDSMKELATHATIPVINGLDNKEHPCQVITDLMTIQEHKGKLKGLQFVYLGDGNN
                     NMAHSYLLGCGIVGMNIRIVAPKQYWPEKYFREEAKKFGVTVEVTDKLQNATSGADVI
                     ATDTWVSMGDEAEKEKRIKDFKGYTIDTAMMCTAKPDAIFLHCLPAYYGYEVTKDVAY
                     GSQSVIFDEAENRMWAQIGIMVTLYRHMFP"
     gene            complement(2124..2804)
                     /locus_tag="VN377_02840"
     CDS             complement(2124..2804)
                     /locus_tag="VN377_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HWR27356.1"
                     /translation="MLTLVLAEAEVERMPAELCNHPAVIGHARQRGKHPRQILLDSNY
                     HHAAMAHLPEGRRRGRPDITHLFLLTALESIANKQGQLNIRIHTRHDQMITVNPKTRI
                     MRNYERFLGLLEQLFETHVVPDTKQPLLSLTENVTLKQVIEQQHADLVIVLSKDGTLV
                     ALPQYLKKVNKQKKDHLLCVIGGFPSGQLHADLSAIATDVISLYSELLPAWTVASELL
                     VNYGNMCR"
     gene            complement(2814..3881)
                     /locus_tag="VN377_02845"
     CDS             complement(2814..3881)
                     /locus_tag="VN377_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HWR27357.1"
                     /translation="MVEVNIKELLSTYDTKNLTVATVCSHSSLQIFHGARKEGFKTLG
                     ISVGEVKKFYDAFPLGKPDEFLTVDNYKDIAKYADELRQKNVIVIPHGSFVEYLGPKN
                     FLNLQVPTFGNRHVLMWESDREKERVWLEGAGITMPKEIKEPRNIEKPVIVKYHGAKG
                     GKGFFIAKTFAEFSKRVKHDKPYTIQEFVMGTRYYLHYFYSPIQDNAYKLTKGSLQLL
                     SMDRRDETTIDEVQRLGSIAELEELGIHPTFVVTGNIPIVMRESLLPQVFKMGEAVVE
                     KSLELFGGIIGPFCLETVVTEDLEFKVFEISARIVAGTNPYISGSAYSDLIEPDLSTG
                     RRIAQEIHYAIKQNQLEEILS"
     gene            complement(3966..4316)
                     /locus_tag="VN377_02850"
     CDS             complement(3966..4316)
                     /locus_tag="VN377_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellum operon transcriptional activator EarA
                     family protein"
                     /protein_id="HWR27358.1"
                     /translation="MLDGEKTVISPELLKPLIIRSLRRSSVRKKIADYLFEISPSGSY
                     TSEIAYNVKTTPTNVIGAIRGMNSRYRGDESLMSLNLVEQIEGGKHRDIKLYRITDLG
                     KEIVEGLRDNKKRF"
     gene            4461..4673
                     /locus_tag="VN377_02855"
     CDS             4461..4673
                     /locus_tag="VN377_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27359.1"
                     /translation="MSIKKELLNELTEHQLKQLAESKGIIFKLNTVQKKYYQNWAERD
                     KLVDLMTTNEHITVSEIEKFIHAKKF"
     gene            complement(4678..5346)
                     /gene="nth"
                     /locus_tag="VN377_02860"
     CDS             complement(4678..5346)
                     /gene="nth"
                     /locus_tag="VN377_02860"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWR27360.1"
                     /translation="MNKPLSHLDEIFSLLKKELHQYGQPIVSRSKPDIPDTPFVTLIS
                     CLLSLRTKDEVTAQASHRLLTKYHTPQEIVQLTEKQIQTMIYPVGFYKTKARRIKDIS
                     KTLLEKYDGKVPDDFDALLTLKGVGRKTANIVMVYGHKKHGYLPIDTHCHRIPNRLGW
                     IKTKTPEETEQALIKVLPPEYWDDFNDLFVRFGQTICVPISPFCNKCPLQKYCKKVGV
                     LTSR"
     gene            complement(5419..6102)
                     /locus_tag="VN377_02865"
     CDS             complement(5419..6102)
                     /locus_tag="VN377_02865"
                     /inference="COORDINATES: protein motif:HMM:NF045529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Heimdall-CTERM domain-containing surface
                     protein"
                     /protein_id="HWR27361.1"
                     /translation="MMKLRNFGIVMISLVILLGSCFTAAATSVSDGTGDVWHYSYAGT
                     AWSWAGNIANKPNIDITEISYTVNDNKITLKLEVSGTIQTSEKVGYYLWYNSTDTIYV
                     MTYMNGTGAGYGMKGVMNFTSGNVTVSGDTLSVVLDVLGDTSKVELWGYAVEYTTFGD
                     QTAEWWGDWAPNSKLPFTPGTGGTTDGNTTDGNNTNGNNTGTKTPGFEVIPVIAAVAI
                     AAILLRRRR"
     gene            complement(6210..6755)
                     /locus_tag="VN377_02870"
     CDS             complement(6210..6755)
                     /locus_tag="VN377_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27362.1"
                     /translation="MNEAPIKEEKIPTYNKKTIGLLIVLVIGGIVLGLILSNIFVTET
                     NERIQHFNDEFGDYIPPDFPFRVTPLTTAEVFLPTLGMIVVCISMFLLIGLIAVYFKV
                     FAKTKSKYIAGLLFFLVPLFIQSIFSVSTLRSLFVSPAIPFGDIRESIGFEWGGLGQI
                     LVVVSIFESIGLSILLYLSNE"
     gene            complement(6780..7082)
                     /locus_tag="VN377_02875"
     CDS             complement(6780..7082)
                     /locus_tag="VN377_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWR27363.1"
                     /translation="MDSLKNVLYWLIANSLGGYNRGRILEELFQKPQNANELSKSLEI
                     EYKTIRYHLKVLEENGILTSVGGGYGKTYFPTETLETNKQYFAEIWDKIGKKSNKE"
     gene            7211..9364
                     /locus_tag="VN377_02880"
     CDS             7211..9364
                     /locus_tag="VN377_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWR27364.1"
                     /translation="MEKIIHKKRKSYAIVLILLISSLSPLFSILSTATVTTNSRSEQS
                     TNNMADDTEYWALLVGAGDYAENPMEDRPDMILEVNDFYAHLLHSDWWPADHIKVLTG
                     EDATVVNIIAGLRWLDRMEDSNDISLVYLSTHGFPLGFDIPPKDEADGTDEALVTYWG
                     FSFPSLVIWDDELNVLLNRLESKGVCLIVDSCYAGGFNDPPNWNITDIPTDSQHQMAY
                     GAEEWAKGFGEDVRGQKRVVLMGSCEDEEAVSGGFGPYLVDGIRGYADSNGDGIISAE
                     EVFYYAQPRSAQMQHPTLYDGYDGELPIMTVQSSEQSVDTNRFVQINTRKDDTNGDPL
                     LQSVETSILCGYVQDAATNVSIENAVVSVRGRINEYEFYENQTTTDSTGFFQMHTPAI
                     RLRATASSGGYCDRSVGPYQMEENETQWANISLYERPQETATVCGFIMDGETTDPVEL
                     ANVSLSWEGSEHQYYENMTTTENNGFYHLNVAAGNISLNIEKEGYFSEYIEELSVDDS
                     ETLWVNVSLHPCPAETAIVCGYLSDNDTGEPLSGAWLEFEWFDAETGSSYGKETQTNT
                     SGFYSITIAPGEIYMYIHGMGYEYYDPYRHDAFENTTVWLNCSLTRSTIEVEFNKPLN
                     ALYLKNQRVMPWKTPRIIGPLDVAAYIPGGWGEPGDAEKVEFYVDGVLQATLTEQPYN
                     WTWSQRSVGKHILKVIAYDFNGDSASKEIEVTKFL"
BASE COUNT         2565 a         2067 c         2148 g         2668 t
ORIGIN      
        1 cctatggatt ttttaaaatt ccgcaaagaa aatcaaagat ttttattaca tataagatga
       61 agaaagagaa cttttttaag gacaaaacaa aaaaaatctt atcgctttct taaaaatcaa
      121 agaggttttt ttgtttggtg agctgattta tcgacgaaaa attttcaaga atcggttcga
      181 cacggtccct ggaaaactga tgatcgtcac agagcaggcg aataaccgct gaggaatcag
      241 gcttcgacca ctggagcgaa tactcccttg ttatggtcgg ctcgagaaat atttttctga
      301 cgatagcgat ttcatcatag gtaaaattcc tttcaacctc tggaatgcta ccgaaggcgt
      361 tctctatggt ccctttttcc ttaaggagtt tgaggctttt tttcgggccg tatcctttaa
      421 tgccgtcatt aaaatcagtc ccgatgagga tggcgatatc tacgagttgt tctcgggaga
      481 tccccagttt ttgtagacca ggttcaagac ggatccgctc tgggtgaacc gcggtatagg
      541 cttgtttgtt tgggagtttc cgtttttctg aggatgtcag gtttcttact aacacaggtg
      601 ctccaaataa aagacagtca aaatcctgcg accctaccgc gaatgcatcc ccttttcgaa
      661 ccatgtagct cgcctgcgat tccccctcag aaggcgcctg gatataaggg attccgagcg
      721 cgtcaagcag ctgtttactc tgctcaatga tctcagcggt cacccgcgag gtctgctgtg
      781 cttttgattt agctttttcc aggtctcctt tttcaagggc ttctttccat tcccgttccg
      841 cttgttcttt gcgttcacgt cgttgctgga tggtccgcgc tttgaacgga tggggaactc
      901 catcgaacac gtacaccggg cggatcttgg ctgccaccat gtttgaggtg cgatacaaca
      961 gaccagataa atgcgacgtg attctcccct gtgaatcttt cagaggagtc ccatcacgct
     1021 gccggatact ggagagaaac tgatggagga tattgtatcc atcgagcgca acgacacggt
     1081 ctttgaaatc ctcaaacgcg catggttcct tggtgaataa atctccgagg tcgacaccca
     1141 tgaacgtacc acatcttttc aagggataaa tgcctttctt tccacccgat atgtttaaag
     1201 aaggaaaacc aattcccttg ttcgatgaag cgagatctta tctccatgct ggatgtgaaa
     1261 gatgacctta acgacatcat aaaactcagc ataaaactca aacaacaatc aaagaaagga
     1321 aaacgcatcg aatatttgaa aaggaaaacc cttgggatga tctttgagaa acccagcaca
     1381 cggacacggg tttcttttga gacagggatg acgctccttg gtggtcatgc gatcttcctt
     1441 tccagtaatg acatccaact cgggagggga gaaagcattc aggataccgc gcaagtactg
     1501 tcccgctatg tcgacatgat catgtatcgt gcgaagagca atgactcgat gaaggaactc
     1561 gcgactcatg cgactatacc ggtcatcaac gggctcgata acaaagaaca cccctgccag
     1621 gtgatcacgg acctgatgac cattcaagaa cacaaaggga aactgaaagg attacagttt
     1681 gtgtacctcg gcgacgggaa caacaacatg gcgcattcct atttactcgg atgcgggatc
     1741 gttgggatga acatcagaat cgtcgcacca aaacaatact ggccggagaa atacttcaga
     1801 gaagaagcaa aaaaattcgg agtgactgtc gaggtgaccg ataaattaca gaacgcaacc
     1861 tccggtgctg atgtcattgc aaccgacacc tgggtctcaa tgggcgacga ggcggaaaaa
     1921 gaaaaacgga tcaaggattt caaaggatat acgattgata cggccatgat gtgtacagca
     1981 aaaccagatg cgatctttct tcactgtctc cctgcatatt acggttatga ggtgaccaaa
     2041 gatgtcgcgt atgggtcgca atcagtgatt ttcgatgaag cagagaacag gatgtgggcg
     2101 cagattggca tcatggtgac gctttaccga cacatgttcc cataattgac tagaagctca
     2161 cttgccacgg tccacgctgg aagcagctcg gagtagaggg aaatcacgtc ggtggcgata
     2221 gcactgagat ccgcatggag ctgaccggag ggaaatccac cgatgacaca gagaagatga
     2281 tcctttttct gcttgtttac ttttttaaga tattgtggga gcgccactaa tgtaccgtct
     2341 ttggatagta cgataacaag gtctgcgtgt tgttgttcaa tgacttgctt taaggtaaca
     2401 ttctccgtta acgaaaggag tggttgtttt gtatcaggga cgacgtgtgt ctcgaagagt
     2461 tgttcgagga gtccaaggaa ccgttcgtag ttccgcatga tccgtgtctt gggattcacg
     2521 gtgatcatct gatcgtgtct tgtgtgaata cgaatgttta gctggccttg tttattcgcg
     2581 atggattcga gagcggttag taaaaaaaga tgggtgatat cagggcgtcc ccgtcgtctc
     2641 ccctcaggta gatgcgccat cgcagcatga tggtagttcg aatccagaag tatctgtctt
     2701 ggatgtttcc ctcgttgtct tgcgtggccg atcaccgctg gatgattgca cagttccgca
     2761 ggcattcgct ctacttcagc ctctgcaagc accagggtta acatgccatg gcctcagctg
     2821 aggatctctt caagttggtt ctgtttgata gcatagtgta tttcttgtgc gatccgtctg
     2881 cctgtggaaa gatcaggttc gatcaggtca gaatacgcag aaccagagat atacgggttt
     2941 gttcctgcga cgatgcgtgc tgatatctca aatactttga actcgagatc ctctgtgacg
     3001 accgtctcaa ggcagaacgg gccgatgatg ccgccgaaca gctcaagtga tttttccacg
     3061 accgcttccc ccattttgaa gacctggggc aggagtgatt ctcgcatcac aatcgggatg
     3121 tttccggtga ccacgaacgt gggatgaatc cctagttctt cgagttctgc tatcgaaccg
     3181 aggcgttgca cctcatcgat ggtggtttca tctcgacggt ccatggagag caactgaaga
     3241 cttccttttg tcagcttata tgcattatcc tggatcggtg agtagaaata atggagatag
     3301 tacctggtgc ccatcacaaa ctcctggatc gtatagggtt tgtcatgttt cacccgtttg
     3361 gagaactctg cgaatgtttt tgcgatgaag aatcctttcc cacctttcgc gccatggtat
     3421 ttcacgatca ctggtttttc gatgtttcgg ggttctttaa tctcctttgg catcgttatt
     3481 ccagcacctt caagccagac gcgttctttc tcgcggtcgg attcccacat gagcacatgt
     3541 cggttcccaa acgtgggtac ttgaaggttc agaaaattct tcggcccgag atactcgaca
     3601 aacgacccat ggggtatgac gataacgttt ttttgccgaa gctcatcagc atatttcgcg
     3661 atatccttgt agttgtcaac ggttaaaaac tcatctggtt tgccaagtgg aaacgcgtcg
     3721 tagaatttct ttacttcacc aactgagatg cctaatgtct taaacccttc ttttctcgca
     3781 ccatgaaaaa tctgaaggct ggaatgcgaa cagactgtcg cgaccgtcag gttttttgtg
     3841 tcatacgtgg agaggagttc ttttatgttc acttcaacca tttagtatcc aactcccaat
     3901 acgaggataa cgcagcccct ttttttatca gtttcgctgg aaaaaaggga caaaaaggtt
     3961 gatagttaga atcgtttttt gttgtctcgt aggccttcga cgatttcctt gcctaaatca
     4021 gtgattcgat acaacttgat atcccgatgt ttgcccccct cgatctgctc aaccaaattc
     4081 aggctcatca atgattcatc accacgataa cgagaattca tcccgcggat cgcaccgatc
     4141 acgttcgtcg gtgtggtctt gacgttgtac gcgatctctg aggtgtaact gccgcttgga
     4201 ctaatctcaa acaggtaatc tgcgattttc ttccgcacgc tacttctccg aagcgacctt
     4261 ataataagag gttttaacag ctctggagaa ataactgttt tctctccatc cagcatccca
     4321 ttccacttag tgtgtttgga attgcgtttc ccattaataa actaatcggt tcacgttcat
     4381 cgtacagacg acgagacgat ggctgaaacg aaacagataa atgtctgttc ttgtttaccg
     4441 gatggtagga tgggatacgt atgtcgatca agaaagagct tttaaacgaa ttgacagaac
     4501 atcaattgaa acaacttgcg gagagcaaag gcattatctt taaattgaac actgttcaaa
     4561 agaaatatta tcagaattgg gctgaaagag ataaactcgt tgatctgatg acgacgaacg
     4621 aacatatcac ggtctccgaa atagagaaat ttatccacgc caagaaattc tgagccatca
     4681 tcggcttgtc aagactccga cttttttaca atatttctga agagggcatt tgttgcagaa
     4741 tggtgatatc ggtacgcaga tcgtctgtcc gaatcggaca aacagatcat tgaaatcatc
     4801 ccaatattcc gggggaagga cttttattaa tgcttgttcg gtttcttccg gcgtcttggt
     4861 ttttatccat cccaatctgt tcgggatccg atgacaatgg gtgtcgatag ggaggtaccc
     4921 gtgtttctta tggccataga ccatgacgat gttcgcggtt tttctgccga ctcctttcaa
     4981 tgtgagtaat gcatcaaaat catcaggtac ttttccgtca tatttctcaa gtagtgtttt
     5041 ggagatatcc ttaatccttc gtgcctttgt cttatagaac ccgacagggt aaatcatcgt
     5101 ctgtatttgt ttttctgtca attgtacgat ttcctgcggg gtatgatatt tcgttagtaa
     5161 acggtgtgac gcctgtgcgg tgacctcatc tttggtccgt aggctcaaaa gacaggaaat
     5221 taaagtcacg aatggagtat cagggatatc tggtttcgat cgcgacacaa tcggctgacc
     5281 gtactggtgc aactcttttt tgagcagaga aaagatttca tcgagatgcg atagtggttt
     5341 gttcataaga aaaacgaaag agtctgtctt taataaaata attcattcgt aaaagaaaag
     5401 aaaaaaaaat gatgcgagtt atcgtcgtcg tctcaacagg attgctgcaa ttgctacggc
     5461 tgcgatgacg gggatcactt cgaaacctgg tgttttcgtc cctgtgttat ttccattggt
     5521 gttattgcca tcggtggtat taccatcagt agttccacca gttcctggtg tgaacgggag
     5581 tttcgagttt ggcgcccagt caccccacca ttcagcagtt tgatccccaa atgtggtata
     5641 ttcaacagca tatccccaca gctctacctt tgatgtatca ccaagaacat caagaacaac
     5701 actgagggta tcacctgaga cagtgacatt acctgaagta aaattcatta ctcctttcat
     5761 tccataacca gcacctgttc cgttcatata tgtcatcaca tagatggtat cagtactatt
     5821 ataccaaaga taataaccaa ctttttcaga tgtttgaata gttcctgata cttccaattt
     5881 cagtgttatt ttattatcat tcaccgtata ggagatttca gtaatatcga tgttcggttt
     5941 atttgctata tttccagccc aggaccatgc ggttcctgca tacgaataat gccatacatc
     6001 acctgttcca tctgaaacac tcgtggccgc tgccgtgaag cacgaaccta ggagtattac
     6061 caagcttatc attactatac caaaatttct cagtttcatc ataatatgcc tctgaagaca
     6121 ggaaaagaac gcagctgtct taaagttatc gtaaaaaaag atgcagtatg atttaatctt
     6181 atattcatta tgatgtattg ttgatagaat cattcattgc tgagatataa taagatacta
     6241 agtccgatgc tttcaaatat cgacaccacg acaaggatct gacctaatcc accccattca
     6301 aatccgatgc tttcacggat gtcgccgaat gggattgctg gtgaaacaaa taaagatcgt
     6361 aaggtgctta ctgaaaatat agattgaata aataaaggaa ctaaaaagaa caataaccca
     6421 gcgatgtact ttgattttgt tttcgcgaat accttgaaat ataccgcaat cagaccgatt
     6481 aagagaaaca tgctgatgca gacgacaatc ataccaagcg ttggaagaaa cacctcagcg
     6541 gttgtaagcg gtgtgaccct gaagggaaaa tctggaggta tataatcacc aaactcgtca
     6601 ttaaaatgtt gtattctttc atttgtttca gtcacaaaaa tgtttgacaa tatcaaacct
     6661 aaaacaatac caccgataac caacactatc aaaagtccaa tggttttttt gttgtatgtt
     6721 ggtatttttt cttccttgat cggagcttca ttcattctac tcaccttttt ctatgctatc
     6781 tattctttat ttgatttttt cccaatttta tcccaaatct ctgcaaaata ttgcttgttg
     6841 gtttccaagg tctctgtcgg gaaataggtt tttccatatc ccccgccgac cgatgtaaga
     6901 atgccgtttt cttctaaaac cttcaaatga tatcgtattg ttttatactc gatttcaagg
     6961 ctttttgaca gctcatttgc attctgtggt ttctgaaaaa gttcctcaag aattctccct
     7021 ctattgtacc cgccaaggga gttggcgatg agccaataca ggacgttttt tagagaatcc
     7081 atttcataaa cctttttgaa agagaatttg tactcttctt taatatgttt cttaaaattt
     7141 gggaaggatt tgggaacaaa ttacttaaac tcgattcctc ttatttttat ttgtaacagg
     7201 gagtattatt atggaaaaaa taattcataa gaagagaaaa agttacgcta ttgtactgat
     7261 tctgctaata tctagtttgt ccccactttt cagcattcta tcgactgcca cggtgacgac
     7321 aaatagtcga agcgaacaat caacgaacaa tatggctgat gatacagagt attgggcgtt
     7381 attggtcggc gctggtgact atgcggagaa tcccatggaa gatcggccag atatgatcct
     7441 tgaagtgaat gatttctatg cgcatctctt gcactcagac tggtggcctg cagaccatat
     7501 taaagtgttg accggtgagg atgcgacagt cgtcaatatc atcgcagggc tccgatggct
     7561 tgatcggatg gaggacagca atgacatctc ccttgtttat ctcagcaccc acggatttcc
     7621 tctcggtttt gatatccccc ccaaagacga ggcagatggg accgacgagg cactggtgac
     7681 gtattgggga ttttcgtttc cgagtctggt tatctgggat gatgagttga acgtattgtt
     7741 gaatcgtctg gaatcaaaag gagtatgcct catcgtggat agctgctatg ctggtgggtt
     7801 taacgaccca ccgaactgga atatcaccga cattccaact gattctcaac atcagatggc
     7861 gtatggtgct gaggaatggg cgaagggatt tggtgaggat gtccgtgggc agaaacgagt
     7921 tgtcctcatg ggatcctgtg aagacgagga agcagtctct ggtggttttg ggccttatct
     7981 cgttgacgga atacgaggat atgcagattc aaatggtgat ggtatcatct cagctgaaga
     8041 ggtgttttac tatgcgcagc cgcgtagtgc ccaaatgcaa catcctacct tgtatgatgg
     8101 gtacgatggt gagcttccta taatgactgt tcaatcatca gaacaatctg tcgatacaaa
     8161 tcgttttgta cagataaata ccagaaaaga tgatacgaac ggagatcctc tgttacaatc
     8221 cgtggaaacc tcgattctct gcggttacgt ccaagatgcg gcaacaaacg tatccataga
     8281 aaatgcggtt gtatcggtac gtggccgaat caatgaatac gaattctatg agaaccagac
     8341 gacaacggat tccacaggtt ttttccagat gcatactcca gcgattcgac tacgggcgac
     8401 tgcctcttcc ggcgggtact gtgacagatc ggtaggaccc tatcagatgg aggagaatga
     8461 gacgcaatgg gcgaacatct ccctgtatga acgtccacag gaaaccgcga ccgtctgtgg
     8521 ctttatcatg gatggggaaa cgacagatcc cgtggagctg gcaaatgtga gcctgtcctg
     8581 ggaagggagt gaacatcaat attacgaaaa tatgacaaca acagagaaca atggttttta
     8641 ccacctcaat gtcgcagcgg gaaatatcag cctgaacata gaaaaagaag ggtatttctc
     8701 agaatatatc gaggaactct cggttgatga ttccgagacg ctctgggtga atgtctcatt
     8761 acatccatgt cctgctgaaa ctgcaatcgt ctgcggatat ctcagcgaca acgacacggg
     8821 tgagccgctt agcggtgcat ggctagagtt tgaatggttt gatgccgaga ccggctcaag
     8881 ttatgggaaa gaaacccaga cgaacacctc agggttctac agcatcacca tcgcaccagg
     8941 ggaaatttac atgtacatcc atgggatggg ttacgagtac tacgacccgt atcgacatga
     9001 tgcatttgag aacactactg tgtggttgaa ttgttcatta actcgatcga ccattgaagt
     9061 cgaattcaat aaaccactca acgctctcta cctgaagaac caacgtgtca tgccatggaa
     9121 aaccccacgt atcatcggcc ctctagatgt cgccgcgtac atccctggag gctggggaga
     9181 gcctggtgat gcagagaaag tggaattcta cgtcgatggc gtcctccaag cgaccctcac
     9241 cgaacagccc tacaactgga cctggtctca gaggagtgtt ggcaaacaca tcctcaaagt
     9301 catcgcctat gatttcaatg gtgattcagc aagcaaggaa atagaggtca cgaaattcct
     9361 ctagcctttt ctttttttct tttgtttttt tctacgtagt ccattttgtc gaacaacgat
     9421 ttgtatttgg gaataatttg ggaagaaa
//
LOCUS       DATMXJ010000102         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_54594, whole genome shotgun sequence.
ACCESSION   DATMXJ010000102 DATMXJ010000000
VERSION     DATMXJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54594"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1586
                     /locus_tag="VN377_02885"
     CDS             54..1586
                     /locus_tag="VN377_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27365.1"
                     /translation="MAKKGRTTRSFARTAPKSQEKYLIENAKKLQEDPFLILPTCTEE
                     SARYFQKLRKKIAKVHQYCSDEKKLEKLSHKKGLDGALAGTYLLAISEKAPYLAALTF
                     PTGDITYAQRGKADREKLIAVQHFDDPVFRLRGITDIVFKKNLRVYSWDAGYVCTGKT
                     AHPPTEFIDFIRNRIGFSSQKDIISCPHVPPDTARKKESLPLNYLRIEWKPAQILIAL
                     CENCAKSTKNTMFSISKYMLQRNLSDDFAIEVTTQVGQHQELSDAQKSASLKEYLSGG
                     LTDADFIKKISKSQEDSVKTAEEKILVLDGVSYGTDVPRFVEALHPTSYEKTALEFFL
                     TKSQEPLIISKTSPGKILDRFWNQHGGDFLESILDDKEKAASLFQLDDTPSNIVVLAF
                     EYQQRQTILSRLPVYTSLPPLATFIDTVARTYRTFGEKKALTEIKNHPDTPKAKSIAY
                     AFLLALGKNTEEKWKYSKEEIDYGEFLQEYTLRILKVEPEQYTKVLQELLTVSGSSET
                     IA"
     gene            complement(1697..2032)
                     /locus_tag="VN377_02890"
     CDS             complement(1697..2032)
                     /locus_tag="VN377_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellum operon transcriptional activator EarA
                     family protein"
                     /protein_id="HWR27366.1"
                     /translation="MQNEGSIASTLELKRPVAVRALRRSNVRKKIAEYLFEISPNYSY
                     TAEIAYHVRTTPSNVIGAIRGMEERYKEDESLLNLDIVQEKNCGNNVRLYGITDFGKE
                     MIKSVKDKE"
     gene            complement(2215..>4033)
                     /locus_tag="VN377_02895"
     CDS             complement(2215..>4033)
                     /locus_tag="VN377_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27367.1"
                     /translation="LFAYTSAFLCFLGFFVFDLLLLAIDIFFLGQMDGITITLLLVVS
                     IVLPVAVMNLIVYYPKTYARYIQIHSLGDIPEILSYLVMYLKLVPNLENSVKFAAAES
                     STTLASDLRKLIWDMEIRLYHGINDAIIHFAIRWGRWSDYFKRSLHLIRSSIQERDEA
                     SRIITLDRALDVSLEGTKETMNQFANRLHQPTVILYSVGIMIPLSLVAMLPAAGLVGM
                     KITIMQVFLIYDLLLPLFVFMYTRKILLSRPAAFHPSNIPLDHPDLCEINRRKRFFVA
                     FVSGIVIATPGIIFLLIPTIAPFGLSNDTIDFIVSSQGVNSFFPVTLFFIWGVAAAVS
                     LYCLSVYRPYKQVRDEIKQMEKEFSDALYILGKRISEEKSPEESFAYAAQTMQGTRIA
                     EVFCQTSYNLTARHTSFYDALYSTEYGSLHHVYSDRIKAILRLFVEGTKKSQRAVSAS
                     LIRIADHLKQLQEVEKKITDMLYELTSTLRSTVSVFAPLIAGVTLSITTLISTILRSI
                     QVKQSPETIIGIPTTFSASAGSFMTENIKPEYFVLVIGIYLIELVILLTRFTNGLNEG
                     DDTATFMYSLGKTMPVSIAVFSITVILGQYFFSQLVPTV"
BASE COUNT         1213 a          920 c          851 g         1049 t
ORIGIN      
        1 tattaattcc taccacaata tactttaaca cctcactttt atggtctgat cacatggcaa
       61 agaaaggcag aacgacgaga agctttgcac ggaccgcacc gaaatcacaa gagaaatacc
      121 tgattgaaaa cgcaaaaaaa ttacaagaag acccattttt gatccttccc acctgcacag
      181 aggagagcgc aagatatttt cagaaacttc gaaaaaaaat cgcaaaagtt catcaatatt
      241 gttctgatga aaaaaaactt gagaaactct cacataaaaa aggattagat ggcgctctgg
      301 ctggcaccta tctgctcgct atctccgaga aagcaccgta ccttgctgca ctgacgtttc
      361 ccacgggaga catcacctat gcacaacgtg ggaaagcgga cagagaaaaa cttatcgctg
      421 tccaacattt tgacgacccg gtgtttcgtc tccgaggtat cactgacatt gtctttaaga
      481 aaaacctccg tgtgtactcc tgggatgcag ggtatgtctg taccggaaaa accgcgcacc
      541 ccccaaccga gttcatcgac tttattcgaa ataggattgg tttctcttcc caaaaagaca
      601 tcatcagttg tccacatgta cctccagata ctgcgcggaa gaaagaatct ctgccgctga
      661 attatctcag aattgaatgg aaaccagcac agattctcat tgctctctgc gagaattgtg
      721 cgaagtcaac aaaaaacacg atgttttcca tctcgaaata catgttacaa cgaaatctct
      781 cagatgattt tgctatcgaa gtcaccacac aagtgggaca acatcaggaa ctctccgacg
      841 cacaaaaatc cgcttctctt aaagagtatc tctcgggagg tttgacggat gctgatttca
      901 ttaaaaaaat ttctaaaagt caagaagatt cggtgaaaac agccgaagag aagatcttgg
      961 tgcttgatgg tgtttcctat gggaccgatg tacctcgttt tgtcgaagca ctccatccga
     1021 cctcttacga aaaaacagcc ctggagtttt tccttaccaa aagccaggaa ccattgatta
     1081 tctcaaaaac atcccctgga aaaatcttag atcggttctg gaatcaacat gggggagatt
     1141 tcctggaatc aatcctggat gacaaagaaa aagctgcttc gttatttcag cttgacgata
     1201 caccatcgaa catcgttgtc cttgcgttcg agtatcaaca acgacaaaca atattatctc
     1261 ggctccctgt ctatacgagc ctcccgccac ttgcgacatt tatcgatact gtcgcacgga
     1321 cctataggac gtttggtgaa aaaaaggcgc taaccgaaat aaagaatcat cctgacaccc
     1381 caaaagcaaa atcgattgcg tacgcgtttt tacttgccct gggaaaaaac accgaagaaa
     1441 agtggaagta ctccaaagaa gaaatcgact acggtgaatt tttacaagaa tatacattaa
     1501 gaatcctaaa ggtcgaacca gaacagtaca ctaaagtcct gcaagaactt ctcaccgtct
     1561 ctggttcttc tgagacgatt gcatgaagca ttactcatct tcttttttct gctgagttgt
     1621 aaaaaactcc tcgtccatta aatagcaatc attatggagc gatgagaaat atgctgccat
     1681 agacattttt tttcacctac tccttgtcct tcacagattt gatcatttcc ttcccaaaat
     1741 ccgtaatccc atataaacga acattattcc cacagttttt ttcttggacg atatcaagat
     1801 tcaataacga ttcatcctct ttgtaccgtt cttccatccc cctgatcgcg cctatcacgt
     1861 tcgagggcgt tgtccgcaca tgatatgcta tctcagctgt gtaactataa ttaggactga
     1921 tttcaaaaag atactccgct attttctttc gaacattact tcgtctcaag gctcgaaccg
     1981 ccactgggcg ttttaactca agcgttgagg ctatcgatcc ctcattctgc atcccaatcc
     2041 accttcagat tactcatcga taattctatt aataagcgat tttctcatgt aatgtacctg
     2101 gtatatcttc atctacatgt tcatgtacat atccctatag cttcttataa aaaataaaaa
     2161 tgtgacgaaa acggtaaacg aaaaactaca tctcctgtga tgatatcatt ggagctatac
     2221 tgttggcacc agttgtgaga agaagtattg accaagtatc accgttatcg aaaaaacagc
     2281 aatcgaaaca ggcatggttt ttccaaggct gtacatgaag gtcgctgtat catcgccttc
     2341 attgagccca ttggtaaacc gggtgagaag gatcaccaat tcaatgagat agatcccgat
     2401 gactaacacg aaatattctg gtttgatatt ttccgtcata aatgagcctg cggaagcgga
     2461 gaacgtagtt ggaatgccaa tgatggtctc aggtgattgt ttcacctgta ttgaacggag
     2521 gatcgttgag atcaaggttg tgattgaaag agtgactccc gcaatcaagg gagcaaacac
     2581 agaaaccgtc gatcggagcg tcgaagtcag ctcatagagc atgtcagtaa tttttttttc
     2641 cacctcttgt aattgtttga gatgatcagc gatacgaatt aaggatgcgc tgactgctcg
     2701 ttgacttttt ttggtgcctt cgacaaacaa ccgtaagatt gctttgatcc gatctgagta
     2761 gacatgatgc aacgatccgt attcggtgct gtagagtgca tcataaaagc tcgtatgtcg
     2821 ggccgtaaga ttatagctcg tctgacagaa cacctcagcg atccgggtcc cctgcatggt
     2881 ctgcgctgcg tacgcaaaac tctcttcagg ggatttctcc tcggatatcc gtttacctag
     2941 gatgtataat gcatcgctga attctttttc catctgcttg atttcgtcac gtacttgctt
     3001 atatgggcgg tagacactga gacagtacag tgaaacagcg gccgctactc cccagatgaa
     3061 aaataacgtc accgggaaga aggagttcac accctggctg cttacgataa aatctatcgt
     3121 atcattcgat agaccaaacg gagcaatagt ggggatgaga agaaaaataa tccctggtgt
     3181 cgctataacg atgccgctca cgaaagcgac gaaaaatctt tttcgtctgt tgatctcgca
     3241 gaggtctggg tgatcgagcg ggatgttcga tggatgaaat gctgcgggac gggatagaag
     3301 gatcttccgc gtgtacataa agacgaacaa aggcagaaga aggtcataga tgagaaagac
     3361 ctgcatgatg gtgattttca tccccaccaa gcctgctgcc ggtagcatag cgacaagaga
     3421 caacggaatc atgattccca ccgagtacag aatgacggtt ggttgatgga gccgattcgc
     3481 gaactggttc atggtctcct ttgttccttc caacgagaca tccaaggctc gatctaaggt
     3541 gatgatccga gaggcttcat ctcgttcttg gatggaactg cgaatgagat gcagggatcg
     3601 tttgaaataa tcgctccatc gtccccaacg gatcgcgaaa tggataatcg catcattgat
     3661 cccatggtac aatcgtatct ccatgtccca gataagtttc cgtaggtccg atgcgagagt
     3721 agtggatgat tctgctgcag cgaatttcac gctgttctca agatttggga ccagcttgag
     3781 atacatcacc agataactga gtatctccgg gatgtcgcca agcgaatgaa tctggatgta
     3841 ccgggcatag gtttttgggt aatacacgat gaggttcatc accgcaaccg ggagaacaat
     3901 cgatacaacg agcaacaggg taattgttat tccgtccatc tgcccaagaa aaaaaatatc
     3961 aatagcgagt aggagcaaat caaaaacaaa aaaaccaagg aagcagagaa acgcactcgt
     4021 ataggcaaat aac
//
LOCUS       DATMXJ010000103        11059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_38966, whole genome shotgun sequence.
ACCESSION   DATMXJ010000103 DATMXJ010000000
VERSION     DATMXJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38966"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VN377_02900"
     CDS             complement(<1..717)
                     /locus_tag="VN377_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27368.1"
                     /translation="METRDFATYMKQFNECLQKYFPPKDAWTPADDALYKPDDLFRIP
                     MKEGSEMQLKSIKHTFTYQYTNNKVYQNFCKERDFSPTDIKTSDDLEKIPLIPDSFFK
                     DYPVGRDFATWLGNIFTGTLPRIVISQKNPTYDEVINAFNQTGMTVAYSSGTSGRHTF
                     IPRDQRTFNISEYAAAKSVMNMIYPLWEPNMYGYLLMPNPKKTNVYAGKVCTIYFDAI
                     KDVQVAIDREITTELIQMTMT"
     gene            943..2067
                     /locus_tag="VN377_02905"
     CDS             943..2067
                     /locus_tag="VN377_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HWR27369.1"
                     /translation="MPKTGVASLPLHPGKAPRWLFKRMVALSKGISEVIIYEYGTDEL
                     LRRLSDPFWFQAFSCVLGYDWHSSGTTTVTCGALKEALNPMDVGIVLCGGKGNASRNT
                     LSEIETSSEKYNLSTSTIERFQYCSRMSAKVDNSAIQDGHTLYHHVFILSENKKWAVI
                     QQGMNSDTKYARRYHWLSEDVDCFVCEPHNAIVGDDRHEHVLDMTAKQSIMTQNVSVD
                     LVNDHPSHLQEDWATLTRHETQRTLDNWVSPPLKNHSYPHLAMPRTINWQKMKEIYDM
                     HPTKYEELLALKGVGPNTVRALALISDLIYNEKPSWEDPVRFSFTVGGKDGVPYPVDR
                     KAMDESTEMIRTGVEDAKIGEKEKIQALRRLKDFLPRYST"
     gene            2287..4194
                     /locus_tag="VN377_02910"
     CDS             2287..4194
                     /locus_tag="VN377_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR27370.1"
                     /translation="MDNIQIDDLKGGFGVTAVIRNTGGQAISDLVWSIDFGDVAKIGR
                     YSSRTISLLSGYDEIVIRSGFVFGLGFGTVTVVTNEFSIVQNYFILGLFVFLKKGAGN
                     QIINHDMIVNYRAGDQKTLDPADAYDAESTDLISQIYDTLVTFQGNDSQTFSPCLATD
                     WTVSVDSLTYTFHLRPNVKFSNGNDFTAEDVKYSFDRVLTMNAPESGVDWILGQCMDT
                     DSTTILDDHTLQITLTEPYGGFLALLAFTVAAIVDKDYVEANGGVVPGETNIWMKEHP
                     MGTGPYMVKSTENTSEVLLIKNPLYWGGWDGNHVEKVFFRTTPDIGERISALLDGVAD
                     FTYVPYEILWDVIGEEGIVVQPFDSYDVSLLSINTKASNNEFLADGQVRKAFSFAFDY
                     NTAIETAWDGYASRLLGAIPTGMPYYETQNNGQPYYTFDLSMAENILDDAGYLKDYCV
                     NGTLYRFNGVTMRLFYNAGNSEREKMAVMFRDALEDIGIRSSVIAEEWPQLLHRIYTT
                     DDWDMVFVGWGPDYNDPDVYIGPFIGSADIGQDTYNTGYRNDTVDKKILEAKYSADPA
                     VRAAAYTAAFDIYIQEPPYIFLVQQQYIRPMRDWIMNYSYNPAPSLCWNFYDCYKEGL
                     HDKIFSERLLV"
     gene            4589..6829
                     /locus_tag="VN377_02915"
     CDS             4589..6829
                     /locus_tag="VN377_02915"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27371.1"
                     /translation="MIKKTVVVSVTVLILLVTSGIASAHIEVNLPQSPVTMQVQYPGS
                     QSYFDTQLKNISFGFDVLNRSYIDWCVAKNIYINPNQDYTNTRLYSIYNMSMPSSLWH
                     DNWSKVNYILNHKVQNIPYWQIQYAIWYLLDFGDAGLTSAGWSMVENASLYGDSYSPT
                     YFDIIGVIADAGINIQKQLLEVRLIDPNGDSDGDGVRNIDEDVDADGNPNNDDTDADG
                     ISNYLDNDDDGDGILTIIEVTDGTSFGQDVDNDGTPNYLDTNSDGDCKKDACEGREDV
                     DGDGIPNYLDANDKDGPYGDLDHDGLQNYVEDNLGTNKTNSDSDEDSVDDYTETEGGS
                     PIDTDNDTVIDANDPDDDDDDILTIVEVTDGTQFGQDVDNDSIPNYLDTDSDGDGLLD
                     HEEGTADHDGDGVPNYLDPDDLQAPSKVENLSAVDAKDGKINVSWDPATDNVGVNHYE
                     VFRDGTLIQNVTGTIYQDTELINGHSYTYTIRAVDAAGNQGNFSDSAIGTPTKTPSAF
                     HTNPSGNTQTSDSNDAPIANSSAGEPYSGQVNESITFNASYSSDPNQDVLSYYWDFGD
                     GTNSTGKIVIHVYTAVGVYNVTLTVSDSHGKTDSDRTIASVIKEYTPLSQPVLTGPSE
                     GFINIAYNFSMIVNDSESPIQFIIDWADGNISESDHVTADSFYMINHTWTQLGEYNIT
                     ITATDGQDNAITKRTMKIIPQINSGAKIPESNNFLLILLALLALMFLVYFFLLGKRKK
                     DDEEEK"
     gene            6982..7941
                     /locus_tag="VN377_02920"
     CDS             6982..7941
                     /locus_tag="VN377_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWR27372.1"
                     /translation="MDWKKLLPVAGIIILVYILWRFNVGKILAVFSTIDPLYVALCFL
                     SIPFWLIVLNIEWQLILKQQNIHVSFTYSLKNYFIGYFYGFITPGGIGNYLRALYLRQ
                     ESNVPLPKCISNIITFNTVDLITLFILSSIGGLVLLGQYPYLFILSLLFLFSVTAVFV
                     FFLRQKKSKQLFEKLIKTQIFQTIQGFMDDPMETFYEDLPTFKSLRLPFLISFVGWFV
                     FFSELYLIAQLFDIHVPFLTLFFILAIAATIASIPISIYGLGTRDAALIALLSIYSVS
                     PENCISFTLFWFTVFWVTPSIIGAFITLLEHKKLPAKQKIASS"
     gene            8075..10879
                     /locus_tag="VN377_02925"
     CDS             8075..10879
                     /locus_tag="VN377_02925"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27373.1"
                     /translation="MVPGVLRAASVFHNTQASEQIMVSKISKEIEIPPGSDYFIRFDV
                     QNLTLKGQDIGPSTNGLSDTVIAAISKSPRWIQQRLTSQFQNLSDPESYASVLLNSSK
                     QYADEIAFSIACCPLGLVPSALLLKENVESLYEHDQWISYADIIEYDDGMGNYSSTIR
                     YKVLENGTERYFTLPSEIYYWYVVHPKITNEEIDAVYGPLWRDYLVKHNDLGYPLLKE
                     KLSTIQYLWDCQSYSQPGGRLWSICINQHPTAIEAISYWIGKTVPNPAIGDRPGQASV
                     IAHEHNGWCGELQKIAIAAQRAALIPSISASNVGEDHVWREFYERGWHENDNWWSDTG
                     GAVDEPDVYAYGWGKNMSAIYQWRGDGTILDDTARYIHEEDRITVDFKVKDLFSQPVD
                     GARVIVLVKGPKDITFYKNLFSEKIQNLWDKLPEILKGKLFSLLFSKIEERINKIPDS
                     ITGLTITTWNYTDSEGRCSFELGKNLDYIFLIQEGNLKKPWQLARHNTLRSLKTETDK
                     SFIIRLLDASHKPPKMTRQEMPTGDCQFHLTLTGSGYQLEKHFTTGGVGRHASQVNID
                     SFFVDSENFQRYKAGQSFICYNYWNGKDTTLSVSAENQDWYLIFRNYGRQTHVLVDFS
                     VDVSVQTTGDRVQIVTPDTSLFKVPISNVGDTVLISGIATTDYVYLSFDHEPPVIELP
                     VTNGEWSFAWNTSGATLGTHLITVSGGGNTSDERSIQLIDGRPPSLSVGTPVDGAILE
                     QGILSISGQSSDNVAVDHVEVTLNNITKTAVGTTTWNLSWDTTGLPLDEYLLSVTAID
                     TQGLISTQTRWIVLNESGHTWGPQINTLSYTPANPTNTSNMIIYTNVTTTGPFAIHTI
                     LLYCYNGTDTVSYEMYRYGDYPIQSRHEEDPLFNQSNAPIFGVELGQFSTGQTIMFWI
                     VAVDTAHNKIQSEGDSFTIL"
BASE COUNT         3169 a         2393 c         2415 g         3082 t
ORIGIN      
        1 ggtcatggtc atctgaataa gctcagtggt gatctcccgg tcaatcgcga cttggacatc
       61 tttaatagca tcgaaataga tggtacagac ttttcctgcg tacacattgg tttttttcgg
      121 gttcggcatc aataaatacc cgtacatatt gggctcccac aagggataga tcatattcat
      181 gactgatttt gctgccgcat actcagagat gttaaacgtc cgctgatcac gtgggatgaa
      241 tgtatgcctg ccacttgttc cgctactgta ggcaaccgtc attcctgttt gattaaatgc
      301 gtttatcact tcatcatagg tggggttctt ttgagaaatg acaatgcgcg gaagcgttcc
      361 tgtgaaaata ttccccagcc aggtagcaaa atcgcgtcca acaggataat ccttaaagaa
      421 gctatcaggg atgagtggga ttttctctaa atcatcactc gttttaatat cggttgggga
      481 gaaatctctc tccttacaaa aattctgata caccttattg ttcgtgtact ggtaggtgaa
      541 ggtatgctta atggatttca gctgcatctc ggaaccttct ttcatcggga tgcggaacag
      601 atcatcaggt ttgtataagg catcatcagc tggagtccat gcatcctttg gcggaaaata
      661 tttttgtagg cattcattaa attgcttcat gtaggtagca aagtctcttg tttccatgct
      721 atttgcctca gtttgatgca tcatagttat gggaaagaaa cactgtcata ttcgattgat
      781 atgtacctct attattgatg tttttctcgt tttatcccct gatgcgtatg gtctgaagca
      841 agttctcttt tttataactt cccgttttat tttatatcct atagtttcag tgaactctga
      901 aaaaagatta aaaacaaagg tgtccatagg tgggagcgtt ctatgccaaa aacaggagtc
      961 gctagtcttc ctttgcatcc cgggaaagca ccccgatggc tgttcaaacg catggtggcg
     1021 ctttcaaaag gaatctctga ggtgattatc tacgagtatg ggactgatga gcttttacga
     1081 cggctttctg atcccttctg gtttcaggct ttttcatgtg ttcttggata tgattggcat
     1141 tcgtctggta ccacaacggt cacctgtggt gccttaaaag aggcattaaa cccaatggat
     1201 gttgggatcg tcttatgcgg tgggaaagga aacgcatctc gaaacaccct ctctgaaatc
     1261 gagacgtcta gtgagaaata caatctttca acgtcaacga ttgagcgatt ccaatattgc
     1321 agtcgcatgt cagcaaaggt tgataacagt gcgattcaag acggtcatac cttgtatcat
     1381 cacgtgttta tcctctctga aaacaagaaa tgggcagtga ttcagcaggg gatgaacagc
     1441 gacacaaaat atgcacgccg gtatcattgg ctctcggagg atgtggactg cttcgtctgt
     1501 gaaccacaca atgcgatcgt tggtgatgat cgacacgaac acgtgttaga tatgacggcg
     1561 aaacaaagca tcatgaccca gaatgtctct gttgatctgg tgaatgatca tccctcgcat
     1621 ttacaagagg attgggcgac gctcacacga catgagactc aaagaacatt ggacaattgg
     1681 gtctctcctc ctttgaaaaa tcactcgtat ccgcatcttg caatgccgcg gacaattaac
     1741 tggcagaaga tgaaggagat ctatgatatg catccgacca agtatgaaga acttcttgca
     1801 ttgaaaggcg ttggtccgaa taccgtccgt gcccttgcgt taatttcaga tcttatctat
     1861 aacgagaaac catcgtggga agacccggtg cggtttagtt tcacggtcgg cggaaaagat
     1921 ggtgtccctt acccggtcga ccgaaaagcg atggatgaat caacagaaat gattagaaca
     1981 ggggttgaag acgcaaagat cggtgaaaaa gaaaagattc aggcgttacg acgattaaaa
     2041 gactttctcc cgagatactc cacataaatg tattgatatt ttacaatcca cttttcttct
     2101 caaggaaatc aagattaaaa atctttttat agagcgaggt gatataaatt aactgaatgg
     2161 aggaaaagaa caacgaaaaa aatagcaata gtaattgtta gtatattgtt agtgactgtt
     2221 ttttcaaata ctcttttagc taaaaaggga gagaactctc aatcatccac aacaggtttt
     2281 gaagttttgg ataatataca aattgatgat ttgaaaggtg gttttggggt cacagctgtc
     2341 attagaaata ctgggggcca agctatttca gatctagtgt ggtctatcga ttttggtgat
     2401 gtagcgaaaa ttggaagata ttcatctagg acgatttcgc tgctttctgg ttacgatgaa
     2461 attgttattc ggagtggttt tgtgtttggc ttgggttttg ggactgtcac cgttgttacc
     2521 aatgaatttt ccattgttca gaattatttt atacttgggc tcttcgtctt tttgaaaaag
     2581 ggtgcaggta atcaaataat aaatcatgat atgatcgtga attatcgagc tggagatcag
     2641 aaaacactgg atccggcgga tgcatatgat gctgaatcta ccgatttgat ttctcagatt
     2701 tatgatacac tggtcacgtt tcaagggaat gattcgcaaa cgttttctcc gtgtcttgcg
     2761 accgactgga ctgtatccgt tgactcattg acatatacat ttcatttacg ccctaatgtg
     2821 aaattctcaa acggaaatga tttcaccgct gaagatgtga aatattcttt tgatcgagtg
     2881 ttgacgatga acgcaccgga atctggtgtg gactggattc ttgggcaatg tatggatacg
     2941 gatagcacca ctattctcga tgaccatact cttcaaataa cactcacgga accatatggt
     3001 ggatttttag ctcttttagc atttacggtc gccgcgattg ttgataaaga ctacgtggag
     3061 gcaaacggtg gtgttgtccc aggagaaaca aacatctgga tgaaggaaca cccgatggga
     3121 accggtccgt atatggtgaa gagtacagag aatacctcgg aggttctcct gataaaaaat
     3181 cccttgtatt ggggtgggtg ggatggaaac catgttgaga aagtgttctt cagaacaacc
     3241 ccggatattg gagaaagaat ttctgctctt ctcgatgggg ttgctgattt tacgtatgtt
     3301 ccttatgaaa tcctctggga cgtcattggt gaggaaggaa ttgttgtaca acccttcgat
     3361 agttatgatg tttcacttct ttcaataaat acgaaagcga gtaacaatga gtttttggca
     3421 gatggccagg tacgaaaagc gttcagtttt gcgtttgact acaatacagc gattgagacc
     3481 gcctgggatg gatatgcaag tagactcctt ggagcgatac caacaggaat gccgtattac
     3541 gagacacaaa acaatggcca accgtattat acttttgacc tttccatggc tgaaaacatc
     3601 cttgatgatg ctggatacct aaaagattat tgtgtaaatg ggacgctcta tcggtttaat
     3661 ggggtaacca tgcgactttt ctataatgca gggaattcag aacgggaaaa aatggcggtc
     3721 atgttccgag atgcacttga ggatatcggt attcgttcat cggttattgc agaagagtgg
     3781 ccacagcttc tgcatcgaat atatacaacc gatgattggg atatggtgtt tgtcggatgg
     3841 gggccggatt ataatgatcc tgatgtttac atcggacctt tcattggctc tgctgatatc
     3901 ggacaagata catataacac tggatatcgg aacgacaccg ttgacaagaa gatattagag
     3961 gcaaaatact cagcagatcc tgctgtacga gcagctgcgt atacagccgc attcgatatc
     4021 tatatacaag aaccacccta tattttctta gtacaacaac aatacattcg tccgatgaga
     4081 gactggataa tgaattatag ttataatcct gcgccaagct tatgttggaa tttctatgat
     4141 tgctataagg aaggattaca cgacaagatc ttttctgaac gcctccttgt ataactcagt
     4201 gctgttgata tctttgaccg atccaggtaa tcaatggaga caatcatgct tttttttatg
     4261 aaactctcta tagaatggag gttgaggacg caaagatcgg tgaaaaagaa aaaattcagg
     4321 cgttacgacg attaaaagac tttctcccga gatactccaa ataaatgtat tatattttac
     4381 aattcactta attccgtttg atcctaagcg tctttcttct ctgagaaaag gaaaagtcta
     4441 ctactcagag ggatttttat attttcttta ttgattattc ctcttttttc cctcataaaa
     4501 cccgcttttc ttgtatttcg ataagacctg tcaaacaagt cttatgaatc atagaagcac
     4561 atataatttt ttaaggtgag tgagataaat gataaaaaaa acagtcgttg tgagcgttac
     4621 cgttctcata ctgctcgtaa caagtggtat agcatcagcc catattgaag tgaatttgcc
     4681 acaaagcccg gtgacgatgc aggtgcagta tcctggatca cagagctatt ttgacacgca
     4741 attgaaaaac atctcgtttg ggtttgatgt gttgaataga tcatatatcg attggtgcgt
     4801 ggcgaaaaac atttacatta atccaaatca agattataca aacactcgct tgtattctat
     4861 ctacaatatg tcgatgccca gttctctgtg gcatgacaat tggagtaaag tgaactatat
     4921 tctaaaccat aaggtccaga atattcccta ttggcaaatc cagtacgcaa tctggtatct
     4981 tctcgatttt ggtgatgcgg ggttaactag tgctggatgg tccatggtcg aaaacgcaag
     5041 tctctacggt gacagttact cccctaccta tttcgatatc attggggtta tcgccgatgc
     5101 agggataaac attcaaaaac agcttcttga ggtacgtctc atcgatccta atggagactc
     5161 ggatggggac ggagtacgga atatcgatga ggacgttgat gccgatggaa accctaacaa
     5221 tgacgacaca gatgctgatg gaatcagtaa ctatcttgat aacgacgatg atggagatgg
     5281 gattttaacg atcattgaag tcacggatgg aacttcgttt ggtcaggatg tcgacaacga
     5341 tggaacccca aactatcttg acactaactc tgatggcgac tgtaaaaaag atgcctgcga
     5401 agggagagag gatgtcgatg gcgatgggat cccaaactat cttgacgcaa acgataaaga
     5461 tggtccctat ggagatctgg atcacgatgg actacagaac tacgttgaag acaacctggg
     5521 aacaaacaag acaaattcgg actctgatga ggactccgtc gatgattata cagagacaga
     5581 gggtggaagc cccattgata cggataacga caccgtgatt gatgccaatg accctgatga
     5641 tgacgacgat gacattctaa caatcgtaga ggtcaccgat gggacccagt ttggtcagga
     5701 tgtcgataac gacagtatcc caaactatct cgacacggac tctgatggag atggattatt
     5761 ggatcacgag gaaggaacag cagatcatga tggagacgga gtaccaaatt acttggatcc
     5821 tgatgatcta caagcaccat caaaggttga aaacctctcc gcagtcgatg cgaaagacgg
     5881 aaagattaat gtatcttggg acccagcgac cgacaacgtc ggtgtgaacc attatgaagt
     5941 cttccgggat ggaactctga tccagaacgt caccggaacc atttatcaag atacggaact
     6001 gatcaacgga cattcctata cctacacgat tcgagctgtg gatgccgcgg gaaaccaggg
     6061 caacttttca gattcggcaa tcggcacgcc cacaaaaacc ccttcagcat tccataccaa
     6121 tccctctgga aatactcaga cctctgacag taacgacgct ccgatagcaa attcttccgc
     6181 aggggaaccc tatagtggtc aagtcaacga atcaatcacc ttcaacgcgt cttacagctc
     6241 tgatcctaac caggatgttc tctcctacta ttgggatttt ggagatggaa cgaactccac
     6301 tgggaaaatt gttatccacg tctatactgc cgttggcgta tacaacgtga ctctgaccgt
     6361 gtccgatagc catgggaaaa ctgactcaga taggaccatt gcttcagtaa ttaaagaata
     6421 cacgccactc tcacaacccg tactcaccgg tccaagcgag ggattcatca acatcgccta
     6481 taatttcagc atgatcgtca atgattctga gagtcctatt caattcatca ttgactgggc
     6541 tgacggaaac atcagcgaaa gcgaccatgt tactgcagac tcattctata tgataaacca
     6601 cacatggaca cagcttggag aatataacat cacgataacc gcaactgatg gtcaggacaa
     6661 tgcaattaca aagagaacga tgaaaatcat tccacagatt aactctggag cgaagattcc
     6721 tgaatcgaac aatttcctgt tgatactcct cgcactgtta gctctgatgt tcttggtata
     6781 cttcttcctg cttggaaagc ggaaaaaaga cgacgaagaa gaaaaataac tttcttcttc
     6841 tcattaattc tttttcttat tgcctgatac cagtagattc ccgagacgag gaagaaatcc
     6901 tcaggtaaat ctctgagaaa gaatatctag aatgaaacaa agtctacata taccaaaatg
     6961 ctcatatcct cactaccacc tatggactgg aaaaaactac tccctgtcgc tggcattatc
     7021 attctcgtct acatcctctg gagatttaac gttggaaaaa tcctggcagt gttttccacg
     7081 attgacccgc tctacgtagc cttgtgtttt ctgagtatcc ctttttggct tattgtactc
     7141 aacattgaat ggcaattaat cttaaaacaa cagaatatcc atgtcagctt cacctactcc
     7201 ctgaaaaatt atttcatcgg atatttctac ggctttatta caccgggcgg cattgggaac
     7261 tatcttcgcg cactctatct cagacaggaa agcaacgtac cgctccccaa atgcatctcg
     7321 aacattatca cctttaacac tgttgatctc atcactctgt tcatcttaag ctcgataggc
     7381 gggctggtcc tcttaggaca gtacccgtat ctgttcatac tgagccttct ttttcttttt
     7441 tccgtgaccg cggtttttgt tttcttcctc agacaaaaaa aatcaaaaca actgttcgaa
     7501 aagctcatca aaacacaaat cttccagacg atacaaggtt ttatggatga cccgatggaa
     7561 accttttacg aggacctccc cacttttaaa agccttcgcc tccctttcct catttcattt
     7621 gttggttggt ttgtcttttt ttctgaatta tatctgatcg cacaactgtt cgacattcat
     7681 gtcccgttcc tcaccttgtt ctttatcctt gcaatcgcag cgaccatcgc ctccatccct
     7741 atttccatct acggtcttgg aacacgagat gctgccctta tcgcactcct ctccatttat
     7801 agtgtatccc cggaaaactg cattagtttt accctgttct ggttcacggt cttctgggtc
     7861 acccccagca tcatcggtgc gttcatcacg ctcctggaac ataagaaact ccctgcgaaa
     7921 caaaaaatcg cttcatcatg attcattctc aacactacga agtctattta tatgagaaaa
     7981 tcctattaat ttttcaaagg aggctactca tatttcgacg aaggttggtt cagcggtaaa
     8041 aatatgtgtt gtattgtgtg tttcattttc tctggtggtc ccaggagtct tgagagcagc
     8101 gagcgtcttt cataacaccc aagcctctga acaaatcatg gtgtcaaaga tatccaagga
     8161 aatcgaaatc ccaccaggat ctgattattt cattcgattt gatgtacaga accttacctt
     8221 gaaaggacag gatatcggac catcgacaaa tggattatcc gacacggtga ttgcagcaat
     8281 ctcgaaatca ccccggtgga ttcagcagag gttgacgagt caatttcaga atctaagcga
     8341 cccggaaagc tatgcatctg tccttctcaa ttccagtaaa cagtatgcag atgagatagc
     8401 gttttccatc gcctgttgcc ctctggggct ggttccttcc gcactgcttc tgaaagaaaa
     8461 cgtagagtca ttatatgagc atgaccaatg gatttcctat gctgacatca tcgaatatga
     8521 tgacgggatg ggaaactact cttctacgat ccgatacaag gtattggaaa acggaacaga
     8581 aagatatttt actcttcctt cagagattta ttattggtac gttgttcatc caaagatcac
     8641 caatgaagag atcgacgcgg tgtacggacc attatggcga gattaccttg ttaagcacaa
     8701 tgatcttggg tatcccttgc tcaaagaaaa actctcgaca atccagtatc tctgggattg
     8761 ccagtcgtac tctcaacccg gaggacgatt gtggagtatt tgtatcaacc agcacccgac
     8821 tgcgattgaa gcgatcagtt attggatcgg aaaaacagtc cccaaccctg caattggtga
     8881 tagacctggg caagcctcag tcattgccca tgaacataac ggctggtgcg gagagctgca
     8941 gaagatagcg atcgcggcgc aacgggcagc actcatccca tcgataagcg cttccaatgt
     9001 cggtgaggat catgtctggc gggagttcta tgaacgaggc tggcatgaga atgacaactg
     9061 gtggagcgat acaggcggtg cggtagatga gcctgatgtg tacgcgtacg ggtggggtaa
     9121 aaacatgtcc gcgatctacc aatggcgtgg tgatggtaca atcctggatg acactgcacg
     9181 atacattcat gaggaagacc gtatcactgt ggactttaag gtaaaagacc ttttttcaca
     9241 accagtggat ggtgcacgag tcatcgtcct ggtgaagggg ccgaaggata tcacatttta
     9301 taagaacctt ttctcagaaa agatacaaaa cctctgggat aaactccctg aaatcctcaa
     9361 aggaaaactc ttctccctgc tctttagcaa gattgaagaa cgaatcaaca aaatccctga
     9421 cagcatcact ggactcacga tcaccacatg gaattatacc gattctgagg gacgatgtag
     9481 ttttgagttg gggaagaacc tagattacat ctttttgatc caggagggga acctgaaaaa
     9541 accctggcag ctcgcacgac ataatactct gcgcagtctt aagacagaga cagataaatc
     9601 attcatcatc agactactcg atgcctctca caaacccccg aagatgaccc gtcaggaaat
     9661 gccaacgggg gattgtcaat ttcatctcac gctgaccggc agcgggtatc aattagagaa
     9721 gcatttcacc accggagggg tcggcagaca tgcatctcaa gtaaacattg atagtttttt
     9781 tgttgattca gaaaacttcc agcggtacaa agcaggtcag tcgtttattt gttataatta
     9841 ttggaacggg aaggatacga ctctgtctgt ctcagccgag aatcaagact ggtatcttat
     9901 cttcagaaat tacggtcgcc aaacccatgt ccttgttgat ttttctgtcg atgtttctgt
     9961 tcagaccaca ggtgaccggg tgcagatcgt caccccggat acctctcttt ttaaggtccc
    10021 tatctctaat gttggtgata cggttctcat ctctggaata gcaacaacgg attatgtgta
    10081 tctctccttt gatcatgagc ctcctgttat cgagcttccc gttacgaacg gggaatggtc
    10141 tttcgcatgg aatacttctg gagcgaccct tggcactcat ctgatcacgg tttcaggtgg
    10201 tgggaacaca tcagacgaga gatctattca gttgatagat ggacgtcctc cttctctttc
    10261 ggttggcacc ccggttgatg gcgcgattct tgaacaaggg atcctcagta tctcggggca
    10321 gagttcagat aatgtcgctg ttgaccatgt cgaggtcacc ctgaataaca tcaccaaaac
    10381 cgcagttggt acaacaacat ggaatctctc ctgggatacc acaggacttc cgcttgatga
    10441 gtatctcctc tccgtcacag caatagacac ccaaggtctg atatcaactc aaacacgctg
    10501 gattgtcctg aacgaaagcg gccatacctg gggtccccag attaatacct tatcgtatac
    10561 ccctgcaaat cccaccaaca ccagcaacat gattatttat acaaacgtga cgacaacagg
    10621 accgttcgcc atccacacca ttcttctcta ctgctataac ggaaccgata ccgtatcata
    10681 tgagatgtat cgatatggtg actatccgat tcaaagtcgc catgaggaag acccgttatt
    10741 taatcaatca aacgctccca tttttggtgt tgaactcggt cagttctcca ctggtcagac
    10801 tattatgttc tggatagttg cggtggatac tgcccacaat aaaatacaat ccgagggtga
    10861 ctcatttact atcttgtaaa acccaagctg agacttttaa aaacttttaa aagaggatgc
    10921 agcatcccag atactactta tttattgtgg ggaatgaaaa atgagtccaa tagatcaacc
    10981 aaggaagcga gtagacaatc cacctcaaag actgcaaaaa ccatcacgga aatggatata
    11041 cctcgcattg atactcgtt
//
LOCUS       DATMXJ010000104         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_69750, whole genome shotgun sequence.
ACCESSION   DATMXJ010000104 DATMXJ010000000
VERSION     DATMXJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69750"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VN377_02930"
     CDS             <1..524
                     /locus_tag="VN377_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR27374.1"
                     /translation="GASWGVIITYFPLLLTERTTLSLPVIGFLVALWAGVGSITSLSY
                     GRIRSYINRKKLLVASYVTIGILSILLAVFTQIIILILIMILLGVAVFLTYPALFSFV
                     SEVTHESVEGWTFGLTFTFQTGGGTILLFLGGVLSDLYGIWMPFVLLGSISLIFTLVL
                     LINYRKPFAQPKQ"
     gene            568..1470
                     /locus_tag="VN377_02935"
     CDS             568..1470
                     /locus_tag="VN377_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWR27375.1"
                     /translation="MQKHALGLLISIIAVSFASIFILSCQAPPLSIAFYRLFFTTLLL
                     VPLLLLRKKTRDELRTLPKTTVLIMIMIGLILAAHFALWISSLKMTSVASSVILVTAH
                     PVLVAPVSFYFLKEKLSWVNALGIAISLGGVGLLVIGNYGFAAFGLDTLEGNLLALLG
                     GIAAGLYILGGRTLRKTVSTITYAFVVYMVGTIALFLICLTLQAPVYNLASSDYAIIL
                     LMAVVSGIFGHTLYNWSLGYIRASIMSVALLGEPIGSSLLAYAIPWIHQEPSLYTMIG
                     GGIILMGIYLTARTMKEPEMLENV"
     gene            1497..2108
                     /locus_tag="VN377_02940"
     CDS             1497..2108
                     /locus_tag="VN377_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27376.1"
                     /translation="MTSKTNKIIGAVVAIAIIISIAVLVYVNLPRQTETPDDNNDDHK
                     TAPPTLTLIYNDETQNFTFGQLERLESYTAKGGYRTQSGFIKGLGNYTGVNITTLVNM
                     LQPIPPYHYTLQVFSDDGKNMSYNYSTIIGNVDIYNPENGSDPNPIGNGNMTMVLAYQ
                     FEGEWLNESSDGKLRIVFLDEQGSITNSRLWLKMVTSIKIITE"
     gene            2189..2941
                     /locus_tag="VN377_02945"
     CDS             2189..2941
                     /locus_tag="VN377_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27377.1"
                     /translation="MRDIQDLIENVERYKNVKVQKRLKSKKNTVAYVLLNGQPRILKW
                     FVPGLKQNMDNEYTILKKGFSALPIPSPFEKDTENNVLVMSYILGQNICDVINDPITV
                     FEEKEKVVHQLADWFVRFHTFFKTEESFQIRGDATLRNFIQNKGRIWGVDFEESRTGK
                     SSEDLATLCGSLLSTDPMFTDEKFKLCQLFLDAYRSAAPWNVENINAEISYALLERIQ
                     WRPNDEDLLRKYATNIRNKGLQIARFNFQLIK"
BASE COUNT         1064 a          834 c          738 g         1070 t
ORIGIN      
        1 gcggcgcatc atggggcgtg atcatcacct attttccgtt actgctcacg gaacggacaa
       61 cattgtccct ccccgtgatc ggttttctgg tcgctctctg ggcaggggtc gggagtatca
      121 cctccctgtc ctacgggaga attcggtcgt atatcaatcg gaaaaaactg cttgtcgcct
      181 cgtatgtcac cataggaatc ctcagtattc tgcttgcggt cttcacacag atcatcatcc
      241 ttattctgat catgattctg ctaggggtcg cggtgttcct cacctatcct gcattgttct
      301 cctttgtctc tgaggtgacc catgagtcgg ttgagggttg gacgtttggg ttaactttca
      361 catttcagac cggcggagga accatccttc ttttcctcgg tggcgtgctc tccgatctgt
      421 atggcatctg gatgccgttt gtcttactag gttcaatatc cttgatcttc acactggtcc
      481 ttctcatcaa ttatcggaaa ccattcgccc aaccaaaaca atgaagagaa aaacaaatag
      541 cgaggcagtt gattatcccc tctcaacatg caaaaacacg ctcttggtct tcttatttcc
      601 atcatcgcag tctcctttgc atccattttc attctctcct gccaggctcc tccattaagc
      661 atcgcgtttt atcgactctt cttcacaacc cttctccttg tcccacttct tctcctgcga
      721 aaaaaaacca gagatgaact ccgtacactt ccaaagacaa cagtactcat tatgataatg
      781 ataggtctta ttcttgcagc gcacttcgcc ttgtggatct cctcgttaaa gatgacatca
      841 gtggcaagct cggtgattct cgtcaccgct catccggttc ttgtcgcacc cgtctctttt
      901 tattttctga aagaaaaact ctcctgggtc aacgcactag gcatcgctat ctcgttgggt
      961 ggtgtcggat tattagtcat tgggaattat gggttcgccg cgttcggtct tgacaccctt
     1021 gaaggtaacc tcctcgcgct tcttggcgga atcgctgcgg gattatacat cttaggggga
     1081 cgaacgctac gcaaaacggt ttctaccatc acctatgcat tcgtggtgta catggtcgga
     1141 acaattgcat tatttctcat ttgcctcacg cttcaagcac cagtgtataa tcttgcgtca
     1201 tctgattatg ccattatcct gttgatggct gtagtctcgg gcatcttcgg tcataccctc
     1261 tacaactggt cattaggata tattcgtgca tccatcatgt ccgtagcact cctcggagaa
     1321 cccataggat cctcattgtt agcatatgcg atcccctgga tccatcaaga gccatcgctc
     1381 tacaccatga tcggtggtgg catcatcctg atgggaattt atcttaccgc acgaacaatg
     1441 aaagaacccg agatgttaga aaatgtttaa aaacaacatc agcttcagag tcgctcatga
     1501 catccaaaac aaataaaata atcggcgccg tcgttgcaat tgccatcatt atatcaatag
     1561 ctgtccttgt ctatgtgaat cttccaagac aaacagagac acccgatgac aataacgatg
     1621 atcacaaaac agccccaccg acgctcacgc ttatttacaa tgacgaaacg cagaatttca
     1681 cctttggaca acttgaacgt ttagaatctt acaccgcaaa aggaggatat cgaacacaat
     1741 ctggttttat caaaggacta ggaaattata ccggagtgaa tatcacaaca ctagtcaata
     1801 tgcttcaacc aattccacca taccattaca cccttcaggt tttctctgat gacggaaaaa
     1861 atatgtccta taattattcg acaatcatcg gaaatgtcga tatctataat cctgagaacg
     1921 gatctgaccc aaacccgata ggtaatggaa atatgaccat ggttcttgcg tatcaattcg
     1981 aaggagaatg gttgaatgag tccagtgatg gaaaactaag gatcgttttc cttgatgaac
     2041 aaggatccat aacaaattcg aggctttggt tgaaaatggt tacgtctatt aaaataatca
     2101 ccgaataaaa gattaaaact ttctataata ttaatacaat tccaatcgga aggtatttct
     2161 cattctgtga tatacattga tttgaacaat gagagacatc caagatctca tagagaacgt
     2221 tgagagatac aaaaacgtca aagttcaaaa acgtttaaag agtaaaaaaa acacagtcgc
     2281 ctatgtgtta cttaatggtc aaccaaggat cctgaaatgg ttcgtccctg gtttgaaaca
     2341 gaacatggat aatgaatata cgatccttaa gaaaggattt tctgccctgc ccatcccatc
     2401 accttttgag aaagacacgg aaaataatgt tctggtgatg agttatattc tcggacagaa
     2461 tatctgtgat gtcatcaacg atcccataac ggtttttgag gaaaaagaaa aggttgtcca
     2521 tcagcttgct gactggtttg tccggtttca tacctttttt aaaacagaag agagttttca
     2581 aattcgtggt gatgcgacct tacggaattt tatccagaac aagggtcgta tctggggggt
     2641 tgactttgag gaatcacgca ccggcaaatc tagtgaagac cttgccaccc tttgcgggtc
     2701 tcttctgagt accgatccaa tgttcacgga tgagaaattc aagctttgcc aactttttct
     2761 ggatgcctat cgaagtgctg caccatggaa tgtcgagaac atcaacgcgg agatttcgta
     2821 tgcattgctc gagcggatcc aatggcgacc aaacgatgag gatcttctga gaaaatacgc
     2881 aacgaatata cgcaataaag gattgcagat tgcacgcttt aattttcaat taatcaaata
     2941 gttcttgtca tctggggaga aatcttagaa gataattaca gaatgttaag gggattcgat
     3001 gtagttctac tgttgtatgg actatgcaga gtttttgatg tctagggagg tcgttaactg
     3061 ggtgaaatat cgacaattgc acataataag tatagaaaat tattaataca tacgggagtt
     3121 tattttcttc gtaggaagag gaaaggaatt catattattc cccctttgaa aagtataggg
     3181 gaggtagagc gttcatattg tctccgagct tcccctttcc tcttaaaata aaaaaccact
     3241 aatgaaagtg atatgacaat tatcgcattg gtttttatca gaatctcagg atgataatga
     3301 acacttgtta cccgtttcta aacatatatt ttaaaggaaa aaaatgaata cacacataat
     3361 tatctactat caataagtgc ttaattgcac acaaaaatca gataagaatt accagtaaaa
     3421 acctaaacaa tatctaattg ggggtaagca actagattgt ttaaaattgc tcatctcgat
     3481 tggataattt ttatatatcc acctttgcat ccatcccaac gatacctatg gggaaggacc
     3541 gattactccc aattctcgct ctcatattgc ttttcatagc gagtagctcg tcactgtacg
     3601 tctatgcatc gcgaacgacg accgatgagc tgcaggtaaa cgggcaacag tataccctcg
     3661 accagctgtt ttcccttgcg aaaccacgag cgttcaacga ttcgca
//
LOCUS       DATMXJ010000105         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_54608, whole genome shotgun sequence.
ACCESSION   DATMXJ010000105 DATMXJ010000000
VERSION     DATMXJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54608"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VN377_02950"
     CDS             <1..949
                     /locus_tag="VN377_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWR27378.1"
                     /translation="PIFVLYQILSGLIGISLLMAVLTIFLAFIASALAGYLTGLVGSS
                     NCPISGVTVTILLIVSLLMLGLGVTGLQGMAIVIFISAVVCIGGSISGDLLQTMASGQ
                     MIGATPKKLQISMIFGVVAISATIGIIIGVLHQAFTIGSTKLPAPQAFLMKGIVQGIL
                     GGNMLWPYVVAGAVLAFILILIGLPVLPVAIGIYLPFTLSVPIFIGGGIRHVTDVILK
                     KKYGSAGEEEYSDWEVAIKQTGITPKEKAIRTGLLFTAGLVAGEALMGVVVAVLIVLG
                     IQLTLFQTAPWWPGLLIFIFIAILLAYIPIREVISQKKH"
     gene            1049..1660
                     /locus_tag="VN377_02955"
     CDS             1049..1660
                     /locus_tag="VN377_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27379.1"
                     /translation="MTEQFPDFKQPPWVQSPSEVMDWINATHPSKKTKGKKHVPSGEK
                     ILRNLHVRFDLNVEQKKIPPSTIFSKVITMITGNGLVEEGFDLVPTAELVLRGLAKAK
                     IKNLSKIVIDEKTVYHHSDDHSDLRKTIDDFSSITHDIQIGKSIEVTAILKEGTRSIV
                     IVKIMKIHKKKDHAVDIQIKGGIKEELYHTFKNYLTEKLGLKG"
     gene            complement(1995..2138)
                     /locus_tag="VN377_02960"
     CDS             complement(1995..2138)
                     /locus_tag="VN377_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27380.1"
                     /translation="MGIVTKLELPKGLKELLDDLEEAEERNDVCIYKTLRYPPRSTPP
                     ATD"
     gene            complement(2373..2708)
                     /locus_tag="VN377_02965"
     CDS             complement(2373..2708)
                     /locus_tag="VN377_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27381.1"
                     /translation="MKYEIVGIFVCILLVAAAVPTVKSLGNGTIMTTPPHSSDLKQAF
                     IFGRYTNLTAQGEYLTIEAANLWVIYKEPRSFDHFPRGTPVTFEMYNAYGHMFKKIEV
                     LFLHVQLVV"
     gene            complement(2842..2982)
                     /locus_tag="VN377_02970"
     CDS             complement(2842..2982)
                     /locus_tag="VN377_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27382.1"
                     /translation="MPVVKLFNGKEFAGDIIEDRDSELVLEDFSSPKRVIPKRDIFSI
                     DF"
     gene            complement(3172..3423)
                     /locus_tag="VN377_02975"
     CDS             complement(3172..3423)
                     /locus_tag="VN377_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27383.1"
                     /translation="MHKNIRRLDAYHISEEEFRELCKQPAGITQRVVNAVAQYNKMVS
                     LSEHLEGKDGNELLIKEAEEEEAKVFLDGIFDIVQPRDG"
     gene            complement(3544..3930)
                     /locus_tag="VN377_02980"
     CDS             complement(3544..3930)
                     /locus_tag="VN377_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27384.1"
                     /translation="MDNDTRIQHVALECASQQQADTFFIKVLGIPKVKSTMLPKELCS
                     SIFQIERSVQMETYDNGKARFEVFLTTEPRKSSFAHIGIAVDNKADFVTRCQRHGLQP
                     FFIQKEGKQLLFVRDFSMNLFEVLEQ"
     gene            4059..>5107
                     /locus_tag="VN377_02985"
     CDS             4059..>5107
                     /locus_tag="VN377_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWR27385.1"
                     /translation="MRQGAIYHIFIDRFAGFHETKNWDSPVFLGGTIKGIIDTLPYLQ
                     DLGITTLWISPFYTTSAYHGYHITDFYQVDPHFGTQDDIKELIRESHDSDLHIIADFV
                     PNHCSKEHRFFKEAQHDKNSQYYDWFYFTDWPDHYLCFLSVKDIPKLNLNNPQTQDHI
                     INAAKYWLSLGFDGFRLDHVIGPSQTFWEHFTGEIKRQYPHTVLIGEAWMMGIKRHEL
                     QTLQVHHKFHKWLSGDSSDNLFKEYVGSLDGILDFKVQELMKHYIINRTLSENTFRKH
                     LRKHYAQFPEDFLLPTFLDNHDIDRFLFQCGNDKGKLKAAAVVQFSLPQPPIIYYGTE
                     TGMTQTTSQWKIPTNG"
BASE COUNT         1588 a         1231 c          934 g         1354 t
ORIGIN      
        1 cccgattttt gtgctttacc aaatcctctc aggactcata gggatatccc ttctcatggc
       61 cgtcctcacc atcttccttg cgttcatcgc cagcgcactt gccgggtacc tcacgggtct
      121 cgtagggtcc tccaactgcc cgatctcagg ggtgaccgtc accatccttc tcatcgtcag
      181 tctgctcatg ctcggactgg gagtaacagg attacaaggg atggctattg taattttcat
      241 ctccgcggtt gtctgcatcg gcggatcgat ctccggggac ctcctgcaaa ccatggcatc
      301 cggtcagatg atcggagcaa caccaaagaa actccagatc tccatgatct tcggagtcgt
      361 cgccatctcg gccaccatcg gaatcatcat cggagtactc caccaggcgt tcaccatagg
      421 aagcacgaaa ctcccagcgc cacaagcatt cctcatgaaa gggatcgtcc agggtatcct
      481 tggtgggaac atgctctggc cctacgtcgt cgcaggtgct gtccttgctt tcatcctcat
      541 cctcatcgga ctcccagtct tgccggtcgc catcggaatc taccttccct ttacgctttc
      601 cgtcccaatc ttcatcggtg gaggcatccg ccatgtcacc gatgtaatcc tgaagaaaaa
      661 atacggcagc gcaggagaag aagagtatag cgactgggag gtggccatca aacaaaccgg
      721 gatcactccg aaggagaaag cgattcgaac cggactgctt ttcaccgcag ggctcgtcgc
      781 aggagaagca ctcatgggcg tcgtcgtcgc agtcctcatc gtacttggga ttcaactcac
      841 gctcttccag accgcaccct ggtggccagg attgctcatc ttcatattca tcgcaatact
      901 cctcgcatac atccccatac gggaggtaat ctcccaaaag aaacactaaa aaaaaataat
      961 accttgagat gctagtattg gatatgcatt tttcttgata gatttaataa agaaataggt
     1021 attacgacct gataattggg aaggaaccat gacggaacag tttcctgatt ttaaacaacc
     1081 tccttgggta caaagccctt ccgaggtcat ggattggatc aatgcaacac atccgtcgaa
     1141 gaaaacaaaa gggaagaaac acgtaccgtc cggtgaaaag attcttcgaa atcttcatgt
     1201 ccgttttgat ttgaacgtcg aacaaaaaaa gatacctcca tctactattt tttcaaaggt
     1261 cataacgatg attacaggca atgggttagt agaagaaggg tttgatcttg ttccaacagc
     1321 agaactcgta ttacgaggac tggctaaggc aaagattaaa aatctctcaa aaatcgttat
     1381 cgatgagaaa accgtatatc atcattcaga tgaccattct gatctgagga aaactatcga
     1441 tgatttcagt tcgattaccc atgatatcca aataggaaaa tcaatagagg tgacagcaat
     1501 tttaaaggaa ggaacgcgaa gtatcgttat cgtaaaaatt atgaagatac ataagaagaa
     1561 ggatcatgcg gttgacattc agataaaggg aggaattaag gaggaattgt atcacacgtt
     1621 caaaaattat ctaaccgaga aacttggtct aaaaggatga cgaaaagaat tttcaaaggt
     1681 ggaatttata gagccatttc cttcttttaa atctcttttt ttcgtctata gaatctacta
     1741 caacaatata aatgataaca ccagcactca taaggacaaa accaaataat ttaatgaaat
     1801 tactccaatt catcgaaaat aaaatagccc caagaacaat gaaaataatt ccgattatta
     1861 cttctacttc cttcatttca tttccaattt aatgttcgga aatgattcaa aataaattta
     1921 tcctgcgata atgtcaaaac aaaaaacata tctttagaaa cgaatatttc ttgaacaaaa
     1981 aaaaataatt ttggctagtc tgtggctggt ggtgttgatc taggaggata tcgaagtgtt
     2041 ttatagatac acacatcatt gcgttcctct gcctcttcta aatcgtctaa taattccttt
     2101 aacccttttg ggagttcaag cttggtaact atacccattt cttccaactg tttataaaat
     2161 tcctcattct ccatgtcgat catagaaaac tcatgttcca actaatcacc tcttcacatt
     2221 acatatggat acttatgata tataaaattt attatgaaac tataattttt tttagaatgc
     2281 catgtttttt gtgcgaacca aatgagaaaa aaagaaaatg aagaacgaga tttatctaaa
     2341 ttggaattca ggcttgccta aaggttttaa aactagacaa caagttggac atggaggaag
     2401 agtacttcta tctttttgaa catatgtcca tacgcgttgt acatttcaaa tgttacaggc
     2461 gtcccacgag gaaaatggtc aaatgatctg ggttccttat agatcaccca taagtttgct
     2521 gcctcgatgg tgaggtattc accttgtgca gtgagatttg tatatctacc aaagatgaat
     2581 gcttgtttta aatcagacga atgtggtgga gtggtcatta ttgttccatt gcccagtgac
     2641 ttgacagtgg gaacggcagc agctaccaat agaatgcata caaatattcc tacgatttcg
     2701 tatttcatgg tttttatctc cattacacaa taggttaaaa gttaatatca gcttaactaa
     2761 tttaaatgtt tggtaaagat aaaaatcatt tcttctttaa aaacatatcc tgagacccaa
     2821 tcggtccagg taaacgaaca gttaaaaatc gatagaaaaa atatctcttt tcggaatgac
     2881 ccgtttagga cttgaaaaat cttccagtac caactctgaa tcacgatctt ctataatatc
     2941 tccagcaaat tcttttccat taaataattt aacaaccggc atttctcctg aactcctcgc
     3001 tccaccttgc ggcttcgtaa ctattccttc tttaaaaaca tatccttagt cagacagata
     3061 gtatgctgat cggtggcgac atcgagggtg tatcatatca gtttattctt taaaaaccag
     3121 cattaccaaa aatcctgagt gacagacact gtatcatctg aaaaaatctc tctaaccatc
     3181 cctcggttgc acgatatcaa atatcccatc caaaaacacc ttggcctctt cctcttccgc
     3241 ctctttgatc aaaagttcat tcccatcctt cccttcaaga tgctccgaca atgacaccat
     3301 cttattatac tgcgcgacag cattcacaac cctctgtgta ataccagccg gttgcttaca
     3361 gagctcacga aattcctctt ccgaaatatg ataagcatct aacctgcgta tatttttgtg
     3421 catccaagtc cacatcccat cggcaaatga atgcacttct taattaataa aacataattg
     3481 gcagatgata atgcaggagg atagttctag cgtatgtttc catgacacgt aagaaacggg
     3541 tatttattgt tccaatacct caaagagatt catagagaaa tccctgacaa acaacaactg
     3601 ttttccttct ttttggatga aaaacggctg aagcccatgc cgctgacacc gggtgacaaa
     3661 atcagcctta ttatcaaccg cgatgccgat atgcgcaaaa gatgattttc ttggttctgt
     3721 cgtgagaaac acctcgaacc gtgccttccc attgtcatac gtctccatct gtacagaccg
     3781 ctcaatctga aaaatcgatg aacaaagctc ttttggaagc atcgtacttt tcactttcgg
     3841 gatcccaagg accttaataa aaaacgtatc agcctgctgc tgacttgcac attccaatgc
     3901 cacatgttga atccttgtat cattatccat agatatccct caaaaaaaac aaatatgttc
     3961 agagaattta aaacaagctg ctaaaaaaaa agatttcatc catcaaaata ttataaaggg
     4021 aagaacatca tgcatgctca atgagtaacc tggattggat gagacagggc gccatctatc
     4081 acatttttat tgatcgattc gctggatttc acgagacaaa aaactgggac tccccagttt
     4141 tcctgggagg gaccatcaaa ggaatcatcg acacactccc ctacctccag gacctaggga
     4201 tcaccacgct gtggatctcc cctttttata caacctcagc gtaccacgga taccacatca
     4261 ccgattttta ccaggtcgat ccgcatttcg ggacacaaga tgacatcaag gaactcatca
     4321 gagaatccca cgatagcgac cttcacatca tcgcggattt cgtcccaaac cactgctcaa
     4381 aagaacaccg gttcttcaaa gaagcgcaac acgataaaaa cagccagtac tacgactggt
     4441 tctacttcac agactggcca gaccactacc tctgtttcct cagtgtgaaa gacatcccga
     4501 aactcaacct caacaaccca cagacacaag atcacatcat caatgccgcg aaatactggc
     4561 tgagccttgg gttcgatggc ttccgcctcg accatgtcat cggtccatca cagaccttct
     4621 gggaacactt cacaggggaa atcaaaagac aatatccgca cacagtcctc atcggcgaag
     4681 cctggatgat gggcatcaaa agacatgaac tgcagaccct acaagtccac cataaattcc
     4741 acaaatggct ctccggagac tcctcagaca acctcttcaa agaatacgta ggatcgcttg
     4801 atggcatcct ggatttcaaa gtccaagagc tgatgaaaca ctacatcatc aacaggaccc
     4861 tatcagaaaa caccttcaga aaacacctcc gcaagcatta tgcgcaattc ccagaggatt
     4921 ttttactccc cacattccta gacaatcatg acattgaccg gttcctcttc caatgtggta
     4981 acgacaaggg aaaactcaaa gccgcggcag tcgtccaatt ctcactccca cagccaccga
     5041 tcatctacta cgggacagaa accgggatga cccagacgac atcacaatgg aaaatcccga
     5101 caaacgg
//
LOCUS       DATMXJ010000106         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12728, whole genome shotgun sequence.
ACCESSION   DATMXJ010000106 DATMXJ010000000
VERSION     DATMXJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12728"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..963)
                     /locus_tag="VN377_02990"
     CDS             complement(268..963)
                     /locus_tag="VN377_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27386.1"
                     /translation="MKPDFSAREKICFDILGLLEKNESYVVIGDYAVSSYGFPRFSID
                     LDVVIPKTNLSFFHRFMSQEGFSKSKEKSDLAYSDKFERYEKDLVSIDLLINTVESRQ
                     TGYAYPFSYLYSNSEIRQVSGWDPFRKARVKVATREMLIALKIHSLRDADKRDVIMLC
                     YQIPDVSALVKHLRNGPKNILLEHIHELGSVLDDQHKKDSLKGVYSVDDRVFEKTVKN
                     CRRALEELLSQLQ"
     gene            complement(960..1568)
                     /locus_tag="VN377_02995"
     CDS             complement(960..1568)
                     /locus_tag="VN377_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27387.1"
                     /translation="MYGWLYTTFGTTTFTIDDFRSMFPSNQPTKTIHDLIKLHYMKRI
                     RHGTYQIIRPEEYVRTIVSENISQEKIMTQAEKPYAYCESTAVTIWTDGYYWTDFTKG
                     FRPIHIQVDKKAVPYWRDFFRHYDTEYIIAGEHKTMFGVTYVIHPIDKIIVEKKDGDP
                     VISLHETIQFCKKNTLLYKPAMEFLDKKYHLGLFSEYESVAS"
     gene            1741..2091
                     /locus_tag="VN377_03000"
     CDS             1741..2091
                     /locus_tag="VN377_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWR27388.1"
                     /translation="MVDWNDMMMDWDHMMNWWGVPYMGFFMIGLWLVFIVVASLVYRD
                     AEKRGMNGLLWFILVIIPCVGILFLILYLILRNDKPLQSSPPKDALQILDERYAKGEI
                     PKEEYEQKKKDLKS"
     gene            2188..>2564
                     /locus_tag="VN377_03005"
     CDS             2188..>2564
                     /locus_tag="VN377_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27389.1"
                     /translation="MMIGLVIVILVAVSIQQIMADKAAINDLTIHVEDVRLADVGLTS
                     CDLLVTINFTNPTGRDLSIVSARFDVFVADSYVGNSSLQHLSIPSKSSKEHDISLTLL
                     YSDIAHAVIEGIKNKNFDIDISG"
BASE COUNT          700 a          461 c          591 g          812 t
ORIGIN      
        1 cgtgttaagg acgagcccga ggccgatgat atcgccgtcg tctatcgtga tttgtagtgc
       61 ctgtgatggg tcggctgcat ggactggggt gtttgtgggg atcaccaaaa gaaaagtgag
      121 gagaagtgcc agcatgagag aggatatccc gttgagtgtg tcatgttcgt gtttcatgta
      181 tgcgaacaag aattatttgc ttataataat ttttattttt tccatcgtga ttctttgatg
      241 agcggacatg ggacgacaaa ggattgatta ctgcaattga gaaagtaatt cttctaaagc
      301 acggcgacaa ttttttacgg ttttttcaaa cactcggtca tcgacggaat acacgccttt
      361 cagcgaatcc ttcttatgct ggtcatcgag aacagatccg agttcgtgta tgtgttctag
      421 gagtatattc ttcggtccgt ttctaaggtg tttgacaaga gctgatacat ctggtatctg
      481 ataacataac atgatgacgt ctcgtttatc agcatcacgc agagagtgta tctttaaggc
      541 aatcaacatc tctcgtgttg caacctttac tcgtgcttta cggaaaggat cccagccgct
      601 aacctgacgt atctcagaat tcgaatacaa atatgaaaac ggataggcat atccggtctg
      661 tcttgattcg accgtattga tgagaagatc gatgctgaca aggtcttttt catatcgttc
      721 aaatttatca gaataggcaa gatcagattt ttctttgctt ttcgaaaatc cttcctgaga
      781 catgaatcga tgaaaaaacg aaagattggt cttgggtatc acgacatcta aatcaataga
      841 gaaacgggga aatccatatg atgatactgc atagtcacca atcacgacgt acgattcgtt
      901 cttctcgagg agacccagga tatcaaaaca tattttttcc cgtgcggaaa aatcaggttt
      961 catgatgcca ccgactcata ctctgaaaac aaaccaagat gatatttttt atctaaaaac
     1021 tccatcgcgg gtttgtataa caatgtattt tttttacaaa attgtattgt ttcatgtaaa
     1081 gaaataacag gatctccatc ctttttttca acgatgattt tatcaatagg atgtatgaca
     1141 taggtgacgc cgaacattgt tttatgttct ccagctatga tatattctgt atcatagtgt
     1201 ctaaaaaaat ctctccagta cggtactgcc tttttatcga cctgtatgtg gataggtctg
     1261 aagcctttcg taaaatcggt ccagtagtat ccatcggtcc agatagtgac tgcagtgctt
     1321 tcgcagtagg catacggttt ttcggcttgg gtcatgattt tttcttggct gatgttttct
     1381 gagacgatgg tgcggacgta ttcttctggt cgtatgattt gataggttcc atggcgtatg
     1441 cgtttcatgt agtgtaattt gattaaatca tggattgttt ttgttggttg gttgctggga
     1501 aacatactcc ggaagtcatc aatggtaaat gtagttgttc caaatgtggt gtataaccat
     1561 ccatacattt tcgtatatct attcataatt actattatgg taattattag tataaataag
     1621 tatcggtctt tactgggttc tcgaagatgt cacaaggcct cagggttcga tggagaacca
     1681 tagaatcgta cggatatgtt tttaaggaaa gaggtgtttt gaaatgttcg aaggtggcct
     1741 atggtggatt ggaatgatat gatgatggac tgggatcata tgatgaactg gtggggggtc
     1801 ccgtacatgg gattctttat gatcggcctg tggcttgttt ttatcgttgt cgcatcgtta
     1861 gtgtatcggg atgcggagaa gcgagggatg aatggtttac tgtggtttat cctggtgatc
     1921 atcccttgtg tcgggattct ttttctgata ctgtacctga ttcttcgtaa tgacaaaccc
     1981 ctgcagagtt ctcctccgaa ggatgctttg cagatcctgg atgagaggta cgcgaaaggt
     2041 gagataccca aagaggagta cgagcagaag aaaaaagacc tgaagagtta atacgacaaa
     2101 accatcaacc ccataccata aggcatgaat ttctcaggat aggttttcaa ggaagaaatg
     2161 aggcacatga taaaaaaacg atatttgatg atgattggac tggtcatcgt tattcttgtt
     2221 gccgtctcga tccagcagat catggctgat aaagcagcga taaacgatct caccatacat
     2281 gtggaggatg tccgtttagc tgatgttggg ctcacctcct gtgacctttt ggtcaccata
     2341 aatttcacga atccgacggg tcgagatttg tctatcgtat ctgccagatt cgatgtcttt
     2401 gtcgctgata gttatgtggg aaatagcagt ttgcaacatc tgtctattcc aagcaaatct
     2461 tcgaaagaac atgacatttc gttaacactt ttgtattcag atatcgctca tgcggtcatc
     2521 gaaggcatta agaataaaaa ttttgatatc gatatctctg gtga
//
LOCUS       DATMXJ010000107         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33540, whole genome shotgun sequence.
ACCESSION   DATMXJ010000107 DATMXJ010000000
VERSION     DATMXJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33540"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VN377_03010"
     CDS             <1..1405
                     /locus_tag="VN377_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HWR27390.1"
                     /translation="VYEVMEPQLSEDEKQTKNEIAHLFKMLADIDTFDMTYEEKTKFL
                     EETIERVICDNHIYIEEGSKDIIYYHIFRDFLGYGRIDIPMHDDGIEDISSDGPNTPI
                     FIHHRKFEAIESNIYFESEPELNSFLVRLCQVSGKQISIFSPIVDGKLPDGSRLQATL
                     ARTVTKGSTFTIRKFKEIPLTPVDLIEYKSMSTEMAAYFWMAIEDGASILFCGGTASG
                     KTTALNALMLFIPASHKIISIEDTREVNIPHKNWIAGTTRKGFAASDESKTGKDIDMF
                     DLIRAALRQRPRVIIVGEVRGKEAYSLFQAMATGHTAYATVHASTIHTLIQRLENPPI
                     ELPRALLTSLDVIVFQNAVEIGGKMVRRMTSVTEIIKLDSDTNQLIFMAPYAWVSKTD
                     DRFESTGSSKIFNRIKQQNEWSDEQLQHELNNRIIVLEWMRKKKIKSYKKFGSIIAEY
                     KKFPERVLERIQRDTEE"
     gene            1402..2265
                     /locus_tag="VN377_03015"
     CDS             1402..2265
                     /locus_tag="VN377_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR27391.1"
                     /translation="MKSTKYTRFCRQTFSNLFERFNVSETNKNNMLEKADISMVYQEY
                     YSMVLMNIILGFIASFSSTLFLYLLLPSNITALLLLVVTSIVPFMIGMVYLQLPTTKA
                     KSRGKNIDRHLPYVANFINTMSVAGISPSEIFGTLAGIELYGEVQKEAKKITTEIELM
                     GLDAVSALNNAIKISPSRKFKDFLQGIIAVLQSGSNLAPYFDRCVDRYMYDDLSDRKK
                     NLDSLAIMAESFVTTVIAFPLFLVIILSIMGLTSGGISFDFLYIISLVILPMAYGGFY
                     VMMKSGMGEEV"
     gene            2280..>2976
                     /locus_tag="VN377_03020"
     CDS             2280..>2976
                     /locus_tag="VN377_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR27392.1"
                     /translation="MKEYNINRKYGKDKLIEVILGVSILLAAFCFVFGFFDIIGSPII
                     TVSFIQPIDYLVFGVLGCIGPLGFYNAMKQKQKREIMDKLPDFLLDIANSSASGMNIY
                     DSMRSASEGDYGRLTNELKMMVAQLSWGISIDEALTNFGERINNNEVKRLAITINKAL
                     EIGGNTASVFNAAAKELDQIRRVEQQRRTEMSMYSIVIFISFFVFLAVILVINGTIFQ
                     AIYDLQGKMAGKAI"
BASE COUNT          905 a          682 c          613 g          776 t
ORIGIN      
        1 cgtctacgaa gtcatggaac cacaactcag cgaagacgaa aaacaaacta aaaatgaaat
       61 tgcacatctc ttcaaaatgc ttgcagatat cgacaccttt gacatgacct atgaggaaaa
      121 aacaaaattt ttagaagaaa ccattgagcg tgtcatctgc gataaccata tctacattga
      181 agaaggttca aaagacatca tctattatca tatcttcaga gacttccttg ggtacggacg
      241 aatagatatt ccgatgcatg acgatggtat cgaggatatc tcctccgatg gaccaaacac
      301 tcctatcttc atccatcacc ggaaattcga ggcgatcgaa tcaaacattt actttgaatc
      361 tgaacctgag ctgaactcat ttctggttcg actctgccaa gtctcaggaa aacaaatatc
      421 gatattctca ccgattgtcg atggaaaact accagatggc tctcgtctcc aagcaaccct
      481 cgcacgtaca gtcaccaaag gttctacctt taccatacga aaattcaaag aaatccctct
      541 cactcctgtc gatttaatcg aatataaatc catgtctacg gaaatggcag cgtatttctg
      601 gatggcgatc gaagacggcg cctctattct attctgcgga ggaacagcaa gtgggaaaac
      661 cacggcactg aacgcgttga tgctgttcat ccctgcctcg cataaaatca tctccataga
      721 agacaccagg gaagtcaata tcccccataa gaactggatc gctgggacca ccagaaaagg
      781 atttgcagca agcgatgaga gcaaaaccgg caaagacatc gacatgttcg atctcatcag
      841 ggcagcattg cgtcaacgac cccgtgtcat catcgtcggt gaggtccgag gaaaggaagc
      901 gtacagcctt ttccaggcga tggcaaccgg gcataccgcg tatgcgacgg tccacgccag
      961 tacgattcat acactgattc aacgactcga aaacccaccg attgaactcc cccgggctct
     1021 cctgacgtcc cttgacgtca ttgtctttca aaatgcggtg gaaatcggag ggaaaatggt
     1081 cagacggatg accagtgtca ctgaaatcat caaactcgac tcagatacga accaactaat
     1141 tttcatggca ccatacgcat gggtctctaa aaccgatgac cgcttcgaaa gtacagggtc
     1201 aagcaaaatc ttcaatcgaa ttaaacaaca aaacgaatgg tctgatgaac aactgcagca
     1261 tgaactcaac aacaggatca tcgtccttga atggatgcgg aagaaaaaaa tcaaatcata
     1321 taaaaaattc ggcagcatca tcgcagaata taaaaagttc cctgaacgag tccttgagcg
     1381 tatacagagg gatactgaag aatgaaatca acaaaataca cacgattttg tcgacagacc
     1441 ttctcaaatt tatttgaaag attcaacgtc agtgagacaa ataagaacaa catgcttgaa
     1501 aaagcagaca tcagcatggt gtaccaagag tactactcca tggttctgat gaatatcatt
     1561 cttgggttta ttgcttcgtt tagttcaacg ttattcctct atcttcttct cccgagcaac
     1621 atcaccgcgt tactccttct tgttgtaaca tccattgttc cgtttatgat cgggatggtg
     1681 taccttcagt taccaacaac aaaagcaaaa tcacgaggaa agaacatcga ccgtcacctt
     1741 ccttatgtcg caaatttcat caataccatg tctgtcgctg ggatttcccc ctctgaaatc
     1801 ttcgggaccc tcgccggaat cgaactctat ggggaggtac aaaaggaagc taagaaaatc
     1861 actacagaaa tcgagcttat gggattggat gcggtttctg cattaaacaa cgcaataaag
     1921 atttccccct cgagaaaatt caaggatttt cttcaaggca tcattgcggt tctccaatca
     1981 ggaagcaacc ttgcacccta ttttgaccgt tgtgtcgatc ggtacatgta tgatgattta
     2041 tctgatcgga aaaaaaacct tgattcgttg gctatcatgg cagaaagctt tgtcaccact
     2101 gtgattgcat ttcccttgtt ccttgttatc atcctgtcga ttatggggct tactagcggt
     2161 ggtatctcct ttgatttcct ctatatcatc tcccttgtta tacttccaat ggcgtatggt
     2221 ggattttatg tcatgatgaa atcagggatg ggtgaagaag tataaagaaa atcagatttg
     2281 tgaaagaata caacatcaat agaaaatatg gaaaggataa actgatcgag gtcatccttg
     2341 gcgtgtcgat cctccttgcc gcgttttgct tcgtctttgg tttttttgat atcataggga
     2401 gccctatcat caccgtcagt ttcattcaac cgatcgatta tctggtcttt ggtgtcctgg
     2461 gctgtattgg accacttggt ttttataacg ctatgaaaca gaagcaaaaa cgagaaatca
     2521 tggataaact ccctgatttc ctgctcgaca tcgctaactc ctcggcatca gggatgaaca
     2581 tttatgactc tatgcggtca gcatccgaag gcgactatgg tcgattgacc aatgagttga
     2641 aaatgatggt agcccagttg tcctggggta tttctattga tgaggcgtta acaaatttcg
     2701 gggagcgaat caataacaat gaggtaaaac gactcgcgat tactattaat aaagcattgg
     2761 aaatcggtgg gaacaccgcc tccgtcttta atgcagcagc aaaagaactt gatcaaatca
     2821 gacgtgtcga acaacaacga cgaaccgaga tgtccatgta tagtatcgtc atcttcatca
     2881 gttttttcgt gttcctcgca gtaattcttg tcatcaacgg gaccatcttc caagctattt
     2941 atgatctcca ggggaaaatg gcagggaaag caatcg
//
LOCUS       DATMXJ010000108         8566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_39022, whole genome shotgun sequence.
ACCESSION   DATMXJ010000108 DATMXJ010000000
VERSION     DATMXJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39022"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..5240
                     /locus_tag="VN377_03025"
     CDS             141..5240
                     /locus_tag="VN377_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27393.1"
                     /translation="MRKTNNIFVKIVVSLLFFVFITTSAAQIFINTNPDTNYSLSGND
                     DRGGLFSTMTWEDLFDNTTKIDTNPPGAGQSDNYLVSNGQVSMTDTYPVWTDPAWTKM
                     KPITITNTAGQILTNTIIYFAITHEPDMQSQYQDICFKHQNNPSTWLNYWIETYNTTT
                     AHVWVKVPTVPIGTSSMYLFYGNPSATSQSSYPGVFGWSAFWADDEKTTNHANNEGTW
                     DPDVAYGNNEFLLAWEEGQPWFPPYTWGFKQEIRASMYDTSGNKIVDDKQIFNDGTTY
                     FRNENPSIDYGGGKYFVAWEHYDTVANPSATTEDIKARTVVRNGDQLQLGNVITVCSA
                     VDCQADANVQFDSVNNRFCVVWEDARNGETNYNIYGRLYDTNGNPVGNEKGIATATYS
                     QCEPWVAFDPVHERYMIVWEEGITPDNGPFSIKAGIFDENLAQIGSTITVATGNDAVD
                     YNFPCVEFSPQTQRFLVTWNNDDISTADWWGNIWGILFDYSGNVVVNTFQIKSGEYVR
                     TDIVSYFSSSFFVSFNSKGTSGSGLIWGKIISSNGELIDGDIKLSVSPSALADWSNIA
                     VGNNNVFVGWEDIRRTYPSPWDDMPDVYSNLWSSSIAGSSVTYSIGTEKQLVLSAHVT
                     SVKITPANFHHWDIFNATYMNGTITFDVLDGMTGALLFQNILPGSNLFNQGILAPTIR
                     LKATFTRSNPSTSPKLDKWSVKWQTNEPPYIPGNPIPTNGTTDVPITSDLSWTGGDPN
                     GDPITYDVYFDQMYPPVKVMGNQTSASYDTGTMSYGTPYYWKIVSWDNHGASTIGPLW
                     HFTTHNDPPNPPGGPNPANGATGVSINTDLSWTGGDPNPGDSVLYDVFFGTTTPPTVK
                     VSENQSSLMYDPGPLSYDTHYYWKIVSWDAHGASTSGVIWDFTTENRAPYPPSSPAPP
                     QGATGISITVDLSWIGGDPDGDPVTYDVYFGTSSSPPKIFGNQSVPTYDLSILSYGTT
                     YYWRIVAWDSHGASAGSPLWHFMTNSLPNEPNNPSPPNHATSVDINADLSWTGGDPDP
                     GDAVLYDVYFGTSNPPPKVAGNQSGTTYNPGTMNYLITYHWKIVAWDSHGASKAGLVW
                     DFTTTYVPNNPPNVPSNPIPQNNSSDDSINTDLSWTGGDPDPGDTVTYDVYFGTTNPP
                     PIVSIGFPSTTYNPGVLSYSTTYYWKIVSWDNRGASTQGPLWIFTTTGVPNNPPYIPN
                     APSPLNHAVGVNIDADLSWTGGDPDTGDTVLYDIYFGTIYPPVKIVGNQSDNFFDPGI
                     MNYGTIYYWQIIAWDNHGASSAGLIWDFTTENEVNIPPYIPSDPQPANHATNVDINED
                     LSWNGGDPDPGDTVTYDVCFGTTYPPPKLISNQSATNYEPGTLIYSTTYYWNIIAWDN
                     HDASTQGSVWDFTTMEEPNNPPYPPSDPSPSDGATNVETTVDLSWIGGDPDGDDVTYD
                     VYFGSTSPPLKVASNQSTPSYDPGTLDYVTTYYWEIISWDIHGLSSSGDIWQFTTRTT
                     PNNPPYTPDDPSPMDGATAVDVDTLLSWTGGDPDQDDTVTYDIYFGTTSPPLKLVSNH
                     TDSSYQPTTLQGNTTYYWKITAWDNHGAATPGLTWSFTTEILGDITPPVVQITKPEKA
                     IYIRNDKVLPFITPLVFFAIDVQVSASDNESGIALVEFYVDETLKANDTTAPYTWTWS
                     ERSLFRHFLKVIVYDNAGHKTEKETEVWKFF"
     gene            complement(5347..6213)
                     /locus_tag="VN377_03030"
     CDS             complement(5347..6213)
                     /locus_tag="VN377_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR27394.1"
                     /translation="MKSILLLSASVSVNSAMLPTRRFVIFLEKMFPFRFLAAKVTRVP
                     LVREIADRMLFKQTNLTVIPVESVVEINLEKSIKTPDSIVLPSQVVDYFIRKTNYRFI
                     MNFCICREANHCKNHPIEFGCLFLGDAARGINPEFGREATVEEALTYVEKCRAEDLIH
                     LIGRDKIDETWLGVGSDGKLLTICNCCSCCCLWKILPDLDPHIRSKVKRMPGVEVTVT
                     ERCIGCGTCTEHCFVRAIHIEQGHAIIGDDCKGCGRCADHCPQKAIRVTITDGQFLQK
                     TIDRIESSVDVL"
     gene            complement(6288..7409)
                     /locus_tag="VN377_03035"
     CDS             complement(6288..7409)
                     /locus_tag="VN377_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27395.1"
                     /translation="MFGEHPSAVSKKPSTKTQAYTTNMIYNIKTGHWTGDDHIGDPSG
                     YGRLNSCDVESIYQPEWDCELWFNIYQTDYDGDDIPYWTEVYTYRTDPTYNNTGEDFD
                     NDTLPIEWEHKWMYDPNIWDNHAQLDMDNDILTNVEEYLVSDWGSDPYRRDLYIEMDV
                     MAEGPLGQNSSVPSYSKELLRTAYNRRNIVYHLDDGCLGGGGEKIPFDRKTYPHEILT
                     TYNTYFLHNDSENWRRSVFRYAMIIYNHYTAPGMAFVGEHQRLYWHVQGINTFVISAR
                     SMQKTSLKLSKSIDFVFACAIMHETGHTFGIDFLFPAGCDNVRTSGPFSLAFWFFGNY
                     KSCMNYRYVYSILDYSDGSHGLFDYDDWSNLDFPFFERK"
     gene            complement(8088..>8566)
                     /locus_tag="VN377_03040"
     CDS             complement(8088..>8566)
                     /locus_tag="VN377_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27396.1"
                     /translation="LEGNFINDNKFGVNQYQSFSSTILSNVISRNTYGIQDEFGERNR
                     FEQNTFDNNTLGIQIMACGKTQVIRNIFVHNERNAYFINEGVYELQAKLSAWNHNDSF
                     VFSQYHILGCTIWRENYWEKPRLLPCSINGELWLWTYWEPHPIPWVAFDWYPAQKP"
BASE COUNT         2476 a         1859 c         1832 g         2399 t
ORIGIN      
        1 gtaatcaatg ttataaacac attgtttcct tgctatcctc agagcaaatc aaattcatag
       61 tagagcggaa gacggtgtcg ttgtcttaaa tatgtttaaa tatacatgag gtataaacaa
      121 atacaatacg ggggacattt atgaggaaga cgaacaatat ctttgtaaaa atagttgtca
      181 gcctactatt ttttgtattc ataacaacct cggctgctca aatttttatc aacactaacc
      241 cagatacgaa ttactctttg tcaggcaacg acgatagagg tggtttgttc tcaacgatga
      301 cctgggaaga tctttttgat aatacaacaa aaatagacac aaacccacca ggtgctggac
      361 aatcggataa ttatttagta tcaaatggtc aagtttccat gacagataca tacccggtat
      421 ggaccgatcc tgcatggaca aaaatgaaac cgattacgat tacaaatact gcgggtcaaa
      481 tacttacgaa caccatcatc tattttgcaa tcactcatga acctgatatg cagtcacagt
      541 accaagatat atgcttcaaa catcaaaaca accctagcac ctggttaaat tattggattg
      601 aaacatataa cacgactaca gctcacgtat gggtcaaagt accgacagtc cctattggaa
      661 caagctcgat gtacctcttc tatggaaatc cctcagcaac cagccaaagt agttatccag
      721 gggtttttgg ttggtcggca ttttgggctg atgacgaaaa aaccaccaat catgcaaata
      781 atgaagggac atgggatcct gatgtggcat atgggaataa cgaattctta ctagcttggg
      841 aagaaggtca gccatggttt ccaccctata catggggctt taaacaggaa atcagggctt
      901 ctatgtacga tacaagcggt aacaaaatag ttgatgacaa acaaatcttc aatgatggaa
      961 ccacttattt taggaatgaa aatccgtcaa ttgactatgg tggtggaaaa tattttgtcg
     1021 cttgggagca ttatgacacg gtcgcaaacc caagtgcaac gacggaggat attaaagcac
     1081 gaacggtggt acgaaacggt gatcaattac agcttggtaa tgtcatcacc gtctgtagtg
     1141 cggtggattg tcaagcggat gcgaacgttc aattcgactc ggtgaataac cggttctgcg
     1201 tcgtctggga ggacgctcga aacggagaga caaactacaa catctacggt cgtctctatg
     1261 acacaaacgg caaccctgtc ggaaatgaaa aagggattgc tactgcgacc tatagccaat
     1321 gtgaaccatg ggttgctttt gacccagttc atgaacgtta tatgattgtc tgggaagaag
     1381 ggatcacccc ggacaacggt ccttttagta tcaaagcagg aatatttgat gaaaacctcg
     1441 ctcagattgg aagcaccatc acggttgcga caggcaatga cgctgttgat tataatttcc
     1501 cctgcgttga gtttagtcca cagacccagc ggttccttgt cacctggaat aatgatgata
     1561 tcagtaccgc tgattggtgg ggaaatattt gggggatact ttttgactac tcagggaatg
     1621 tcgttgtaaa tacgtttcaa ataaaatccg gcgaatatgt taggacagat attgtgtcat
     1681 atttttcttc ctcttttttc gtctcattta atagtaaagg aactagtgga tctggattga
     1741 tctggggaaa gataatttct tcaaatggcg aattaattga tggtgatatt aaattatcag
     1801 tgagcccatc agcgcttgcc gattggtcaa atatcgctgt tggtaacaat aatgtgtttg
     1861 tgggatggga agacattcgg agaacctatc cttcaccttg ggatgacatg ccggatgtgt
     1921 atagtaatct atggtcctcc tcaatcgcag gatcaagcgt gacctattcg attggaactg
     1981 aaaagcaact tgtgctgtcg gctcatgtga cctccgttaa aatcactcca gcgaacttcc
     2041 atcattggga tatttttaac gcaacatata tgaatggtac tatcacattt gatgttcttg
     2101 atgggatgac cggagcacta ctctttcaaa atatcttacc cggttcgaat cttttcaatc
     2161 agggtatcct tgctccaacc attcggttga aagcgacatt tactagatca aatccttcca
     2221 cgtccccgaa gcttgataaa tggagcgtga aatggcagac caatgaacca ccttatattc
     2281 ctgggaatcc aatacctacc aatggtacga ccgatgttcc tatcacaagc gatcttagtt
     2341 ggactggtgg tgatcccaat ggggatccta tcacctatga tgtttatttt gatcaaatgt
     2401 atcctccggt gaaggtgatg ggtaatcaaa cgagtgcttc gtatgacaca ggaacgatga
     2461 gctatggaac gccgtattat tggaaaatag tgtcgtggga taatcacggt gcttcaacca
     2521 ttggtcccct ctggcacttt accacgcata acgatcctcc taatcctcca ggtggtccga
     2581 acccagcaaa cggtgcaaca ggtgtttcta tcaacacaga cctcagctgg accggtgggg
     2641 atccaaaccc tggtgattcg gttctctacg atgtgttttt tggtacgaca actccaccga
     2701 cggtaaaagt ttctgaaaat cagtccagcc tcatgtacga tcctggtcca ttgtcatatg
     2761 acactcatta ttactggaag atcgtttcct gggacgctca tggcgcctcg acctcagggg
     2821 ttatttggga tttcacgaca gaaaatagag ccccttatcc accgagttca ccggctccac
     2881 ctcagggagc aaccggcatc agtatcaccg tagatttgag ttggatcggt ggggatcctg
     2941 atggtgatcc tgtgacctat gatgtatatt ttgggacaag tagctcaccg ccaaagatat
     3001 ttggtaatca atcggtgcca acctatgatt tgtcaatatt gagttatgga acaacctact
     3061 attggaggat tgtcgcatgg gattctcatg gtgcatccgc cggtagtcct ttatggcact
     3121 tcatgactaa ctccttgcct aatgaaccaa ataacccaag tccaccgaat catgcgacca
     3181 gtgttgacat caacgctgat ctgagttgga ctggtgggga tcctgacccg ggggacgcgg
     3241 tcttgtacga tgtctatttt ggaacgagca atccaccacc aaaggtcgca gggaaccaat
     3301 cggggaccac gtacaatcca ggaacgatga actatctgat cacgtatcat tggaagatcg
     3361 ttgcttggga ttctcatggt gcatcaaaag ctggtcttgt ctgggatttc accacgacat
     3421 atgtcccaaa taatccgccg aatgtcccta gcaatccaat ccctcagaac aattcaagcg
     3481 atgactcgat taatactgat cttagctgga ctggtggtga tccagatccg ggtgataccg
     3541 tcacctatga tgtgtatttt ggcacgacca atccgccgcc tattgttagc ataggcttcc
     3601 cgagtacaac gtataatcct ggtgttctga gttattcaac gacgtattat tggaagattg
     3661 tctcatggga taaccgtggt gcgtcaaccc aaggtccact ctggattttt accacgactg
     3721 gtgtaccgaa caacccccct tatattccaa acgcgccttc ccctttgaac catgcggtcg
     3781 gagtgaatat tgacgcagat cttagttgga ccggtggtga tccagatact ggggacacgg
     3841 tgttgtatga tatttatttt ggaacgatct atccaccagt aaaaatcgta ggtaatcagt
     3901 cggataactt ctttgatcct ggtattatga attatggtac aatatattac tggcagatta
     3961 tcgcctggga taaccatggg gcatcttcag caggtctgat ttgggatttc accacagaaa
     4021 acgaggtgaa tatccctccg tatatcccct cggatcctca acctgcgaat catgcgacca
     4081 atgttgatat caatgaggat ctgagttgga atggtggcga ccctgacccg ggtgataccg
     4141 taacctacga tgtgtgtttt gggacgacct acccacctcc aaaattgata agtaaccagt
     4201 ctgcaactaa ttatgagcct ggtactctga tatattcgac gacgtattat tggaatatta
     4261 tcgcctggga caaccatgat gcgtcaacgc agggatccgt ctgggatttt acaacgatgg
     4321 aagagccgaa taatccaccg tacccaccat cagacccatc tccatccgat ggagcgacaa
     4381 acgtagaaac aacggtcgac ctgagttgga ttggtggaga tcccgatgga gacgacgtga
     4441 cttacgatgt gtacttcgga tcgacctctc caccattaaa agtagcatca aatcaatcaa
     4501 ctccatcata cgatccaggt accttggact acgtcactac gtattattgg gagattatct
     4561 cttgggatat tcatggactt tcatcttctg gcgatatatg gcaatttacc acaagaacaa
     4621 caccgaataa tccaccgtat acacctgacg acccttcgcc gatggatggc gcaaccgctg
     4681 ttgatgtaga tactctgctg agttggaccg gtggcgaccc agaccaagat gatacagtca
     4741 cctatgatat ttactttgga acaacatctc ctcctctgaa actcgtcagt aaccatacag
     4801 actcttcgta ccaaccgacg acgcttcagg gaaacaccac gtattattgg aaaatcaccg
     4861 cttgggataa ccatggtgca gctaccccag gtttgacctg gagttttacg actgaaatcc
     4921 ttggcgatat cacaccaccc gtggttcaga tcacaaaacc tgagaaagca atttatatca
     4981 gaaatgataa ggtcctcccg tttatcaccc cgcttgtgtt ctttgccatc gatgtccagg
     5041 tcagcgcttc agataatgag tcaggtatcg ctctcgtgga gttctacgtc gatgagacat
     5101 tgaaagcaaa tgataccact gcgccctata catggacatg gagcgaaaga agcctgttcc
     5161 gccacttcct gaaggttatc gtctatgata atgcgggaca taagacggaa aaggaaacgg
     5221 aagtctggaa attcttctaa agcggaagga ttaaacaaaa gccatgtgat ataaattcct
     5281 ccgaagcagt aatgttttta caggaggtga acggtcagta tttcatttaa taaacatacg
     5341 ttcagctcac aggacgtcga cagatgattc aatccgatca atggtcttct gaaggaattg
     5401 cccatcagta atcgttactc ggatagcctt ctgtggacaa tgatcagcgc accgaccaca
     5461 gcctttacaa tcatcaccga tgatcgcatg tccctgttca atgtgtatag cacgtacgaa
     5521 acaatgctcg gtgcacgttc cgcatcctat acatctctct gtcacggtga cctcaactcc
     5581 cggcattcgt ttcacttttg agcgtatatg cggatcaaga tcgggaagga tcttccagag
     5641 acaacagcaa ctgcaacaat tacagatcgt caaaagcttc ccatcagagc ctacgccaag
     5701 ccatgtctca tcgattttat cccgtccgat gaggtgaata aggtcctccg cgcggcattt
     5761 ctccacatat gtcaatgctt cttctacggt cgcttctctc ccaaactctg gattgatgcc
     5821 tcgtgctgca tctcctaaga agaggcaacc gaattcaatc ggatggttct tacagtgatt
     5881 tgcttcacgg catatgcaaa aattcatgat aaatcggtag tttgttttac gaatgaaata
     5941 atcaacgacc tgtgatggaa gtacgatact gtccggagtt tttatcgatt tctcaagatt
     6001 gatttcaacg acagattcaa ctggaatgac ggtgagattt gtctgtttaa agagcatcct
     6061 atcggcgatt tccctcacga gcgggactcg tgtcactttt gcagcgagga accgaaaggg
     6121 aaacattttt tcaagaaaga tgacaaaccg acgcgttggt aacatagcac tgttcactga
     6181 taccgatgcg gataataaga gtatcgattt catatacata attacaaaga atgatgaggg
     6241 gcgtgaaagg gcatggttca attcacagag ttgaaaacga ttgacggtta ttttctttca
     6301 aaaaatggaa aatcaagatt tgaccagtca tcataatcaa ataatccgtg agatccatct
     6361 gagtaatcaa gaatggaata aacataccta taattcatac agctcttata atttccaaaa
     6421 aaccaaaacg caagactaaa aggtccagag gttcgtacat tatcacaacc agctggaaat
     6481 aagaaatcaa taccgaatgt atgacctgtt tcatgcataa tcgcacaagc aaacacaaaa
     6541 tcgattgatt tgcttaattt tagtgatgtc ttttgcatag atcgcgcgga gatgacaaac
     6601 gtgttgatac cttgtacatg ccaatagagc cgctgatgct cccctacaaa agccatacct
     6661 ggagcagtat aatgattata gataatcatc gcatagcgaa acacgcttct ccgccagttc
     6721 tcagaatcat tatggagaaa ataggtgtta tatgtggtga ggatttcatg aggatacgtt
     6781 tttcgatcaa agggtatttt ttctccacca ccacctaagc aaccatcatc aagatgatac
     6841 acaatatttc gacgattata cgctgttctg agcaattcct ttgaataaga tggaacagag
     6901 ctattttgtc caaggggtcc ttctgccatg acgtccattt caatatagag atctcttcga
     6961 tatggatcag atccccaatc agacacgagg tattcctcga cattggttag aatgtcatta
     7021 tccatatcta attgggcatg attatcccat atgttgggat catacatcca cttatgttcc
     7081 cactcgatag gaagggtgtc attatcaaag tcttctcctg tgttattgta cgtgggatct
     7141 gtcctgtagg tatagacctc agtccagtaa ggaatatcat caccatcata gtcggtttga
     7201 taaatattaa accagagttc acaatcccac tcaggttgat agatactttc aacatcacag
     7261 ctattcaacc gcccatatcc actaggatca cctatatggt catcaccagt ccaatgaccc
     7321 gtttttatat tgtagatcat attcgtcgta tatgcttgtg ttttagtgct tggttttttc
     7381 gaaacagcac ttggatgttc tccaaacatc gttttatcgt ataattcaat agtgacgtca
     7441 acatattcga tatcatcagg tacgttcgca gtagctgtcc aatggatatc atagagatag
     7501 ggagaatcat accaaatagg agatgaaaat tcttctgaat ttatgaaaac ctttacaaaa
     7561 aagtcaggat tgtttagaaa accgatgtca tctaaagttc gaattgaaat aatgtcgaca
     7621 gtaacttcaa tatcgacaag cgggtcaaaa tcatccgagt ttcctacttc gccaccgatt
     7681 ctattaggaa cagatatgtt atttttattg attttattat taatgatatc ttcagttaat
     7741 agaccatcaa cgttcgtaat gcttactaaa aatatactga taacgaatat tttgaatatt
     7801 ttttttatat gtagatatgt gtgtaaatga ttacaaacga aatataagat tatgcagggg
     7861 atgagatacc aactccaagt tcaaatctct ttttctttga aagttatacc tctaatgatt
     7921 ataaattctc ctgtcgggat tacgtgctaa tacttcagtg cctcccgtgg gggaagggaa
     7981 gatctaggat aaaatcccct agaaggattg cttgtcaaat gttcaatgcc tcaatcatac
     8041 tttatcggaa aagatatgaa attctctcat ttcttctctg agagtgatta gggtttttgt
     8101 gcgggatacc agtcgaaggc tacccagggg attgggtgag gctcccaata ggtccacagc
     8161 catagttctc cattgatgct gcacggtaat aggcgtggtt tctcccaata gttctcacgc
     8221 catatcgtac atcctaggat atgatactgg gagaatacga aggaatcgtt gtgattccat
     8281 gctgagagct tcgcttgcag ttcatagact ccctcattga tgaaataagc attgcgttcg
     8341 ttgtggacga agatgttgcg aatgacttgt gtttttccgc aggccatgat ctgtattcct
     8401 agggtattgt tatcaaaggt gttttgttca aatcgatttc gctcgccgaa ctcatcttgg
     8461 ataccgtagg tattgcgact tatcacattc gagaggattg tgctggagaa cgattgatat
     8521 tggttgactc caaatttatt gtcattgatg aaattccctt ccaggg
//
LOCUS       DATMXJ010000109        15954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_22929, whole genome shotgun sequence.
ACCESSION   DATMXJ010000109 DATMXJ010000000
VERSION     DATMXJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22929"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1733
                     /locus_tag="VN377_03045"
     CDS             99..1733
                     /locus_tag="VN377_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR27397.1"
                     /translation="MKKFRSVKLKIFFPLLVILLVVFLTSSLVIIERESKAAKDSLIN
                     TATSFSSLSTAAVIENFVFYNESGFYKFTEVIDNVMKLNDNLITIQVVNVNGKILFDS
                     VEIQTGKYDERTNGERFLENATLIQRAGESVYSLFLNEQTRRVDIIQPFFEDWGRHDY
                     SVRYTFSLASLDTMTNDMIVTISLYSGIFIIVSFLLIFFLFNYFISSPIGELTKGVRR
                     MGKGTLGYEVTIKSKDEIGELATAFNTMSTDLKISRDHLEEYSKNLEKLVAERTKELE
                     EKTRRLEQINQDLIVAREELSVLNKNLEGRVQERTGEVEKLLKQKDEFINQLGHDLKN
                     PLGPLINLIPLLEEKEADPAKKEMLTVLHRNADYMKNLVVKTLELAVLNSPNTRFSIE
                     VLNLFKEVNQIISNKKILFDDNNVKILNNIGDSLLIKADRLRLEELLTNIFENSVKYC
                     QEDCRITLDAKLTNDFITISIKDNGIGMNRDEIDRIFDEFYKADSARHDIQNTGLGMS
                     ICKRIVEKHGGNIWVESPGPGKGTTVFFTFPAALTP"
     gene            1841..2227
                     /locus_tag="VN377_03050"
     CDS             1841..2227
                     /locus_tag="VN377_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR27398.1"
                     /translation="MKKIMLVDDEKDQIYFIKTSFENLFGKEYTIIPVESGEKCFELL
                     ENQGLPHLILLDIMMPRMNGWEVFDRIRANPSWKNIPIIFLTARTDEFAEHAGVLIAE
                     DYIKKPIEIKELKARIDNVLKKVKKQ"
     gene            complement(2285..3268)
                     /locus_tag="VN377_03055"
     CDS             complement(2285..3268)
                     /locus_tag="VN377_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HWR27399.1"
                     /translation="MIFIVRELDSKDHKILYHLFLNSRQSLSSVAKKVGLQKSVVEYR
                     IKRLQTKGIIKNFNAMVDVFKLGFSVYRLYIVLQYASPDKEKEIISHFVNHNNTWSIA
                     STKGRYDLIITILVKSPNYFYAFYEDTLRHYRYYFKEIFFSQLYESFGYKHSLLLNEL
                     TTSHERAYEYRDNGQTVDVDLVDYKILNLLAKNTRMNSVDIASQIKVSTVTIHSRITK
                     LIKSGVIQRYSITMDINKLGLREFIVNLSLRDYNKKNQIITYLSDNPFLWEIHKAIGG
                     YDLEITLYATNFEHFYRVMEDLRKKFPDDIANYDYLYVTEVFKSNILPEKV"
     gene            complement(3389..5200)
                     /locus_tag="VN377_03060"
     CDS             complement(3389..5200)
                     /locus_tag="VN377_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27400.1"
                     /translation="MKNILSILLIILLGVSAIGADAFPIESQRTVTDPQLPMVFDFEK
                     EVAIEINISSVAYGHELLWKANTTGTNYEESAVTYTDGIAYIGSCSTHGAGHDKIFAV
                     DTTTGDIIWSNDTGPGYVGPVIDGDVVYIGSSTHGYDPENEYMYAFDRFTGEQVWKTP
                     IYGGIAESVQYDETKIYFCSGFYETKMYALNKNDGSINWTYETGFSVCANKPMLKDNA
                     VYGAFWDNVGKLFKINATTGQEIWSISLSAGPWDNSITADGLGRIFLAIYYSSTMNAY
                     SEQNGQLLWTYSLHGGPLSFNAYHNGHVFIADTLGYVYALNASTGHLLWETKIGGCCD
                     ISSPTLSGGLLFIGTRDGPDGAFYALNETTGSVLWRYQIGASITAPPSIVDGMMLCGS
                     DGWNMYAFDVGTGGGDWLLHRYDRWNTAYSPVGLETWQYVRAACSSQQDIITCVVTNV
                     YDHQVQNIVLHLPFSASWYTESGELLKENSDTYTLDSLPTGGAQTLLISQEPLFTVTI
                     TKPENALYVANTKILPFFVPILVGSIEVQASVHEVNKSLVDRVEFYIDDELQEIDTMA
                     PYGWLWSQRSFGAHMIKVIAYKNDKSTTDELKVWKIF"
     gene            5332..7647
                     /locus_tag="VN377_03065"
     CDS             5332..7647
                     /locus_tag="VN377_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27401.1"
                     /translation="MMKTFTAVFLGFFLLISTLYIPGIGWETASCLSLEPSSNRSLRL
                     SSVADAVVVSESDPYFSLIGTGLACWYDKDTNSTGLLPLLVHHEGNLTDAQTRFLETF
                     FDSTNQTLLVLGEHLKTSYESIEILGSAPSVAISLATSVFTTASDVLIIPYGTLDAYQ
                     LSLLATPLASYLDMPLLIFDDNAVEIQIACTQLQTTHAYVVGDITLHLENITVTSLTN
                     ETAITDMILTVIKDQFGSINYLTMTNPSDVVPPAVLNITHLSFSDHVINKKIIILGKE
                     FDIVGNDTRNYTIFVPDGLTRIQISGEIFQKHGSLLERFSSIVPLLFMTLTDAQGRIV
                     AYANSMGYDIGKSYVETLSCNASGIYTLHIRVYNGIKGGFFVQRGISFVDANITMMAT
                     LSALSSPHLPLIPKLSSLAPYLAAAHGGLIIANVSWELTDESYSSAAQGSGAGPWYNE
                     SLHHFTNTKVNMTVQQLTRTLETLGTHDLLNGYLSGPAWLTLLADTTMIPMYYYGPSQ
                     EDIPDRGLPSDNPYTLNQSLSVGRLISWDVQDSSVLIARTFFYEALCGEPGSSDDWHS
                     RFHFVFGEGFSETGGVFHQIPYAKEIREYGFTSKVYGDFRNSRQIAEILGIFTGANYL
                     EYLGHGDWFWFPASLYGLDSYSKAFDVAHVKNWVFEKPSLFLSSACLMGRTDGLPPQM
                     NIGLTMLHAGCNGFIGATRETGQESGLTVLENHLIVDDWSVGEALRGEKRIDAELPTF
                     YVRVLYGDPAFNPYEPQHGFSNQGRPILLGR"
     gene            8045..9103
                     /locus_tag="VN377_03070"
     CDS             8045..9103
                     /locus_tag="VN377_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27402.1"
                     /translation="MKRDSNDNFSQAINLFDKTDAIIIMDNKKYQGMLLKRTLMEPTV
                     TLQTKLHTMLTHPPKLTPTASLEEIACAMVENNISTLPVLHNDTLLGIVTADDVIKKL
                     TEQAIGAQPVKTIMCTTPLSISPTETIGKVLHLFRQGHSPYIAVVDHSTVVGAITINT
                     IINKVLHSHSNVEGRDNFESEKRQKLDLPAKSFMNEQPLLLSPETSIRDVHSKMHQFE
                     QPGILIGKDNHLHGIVTHKEFLAPFAEIEKEETIAIQFHHRNSKVEGFDKEQAVHFLH
                     AEFLKNYEKYLEVGYLHVSLEQHKEIKQGLFRVVCEMKLSGKPGVFYATQEGYGPQQA
                     MRNAFLAIEHQINKVKKT"
     gene            complement(9137..9811)
                     /locus_tag="VN377_03075"
     CDS             complement(9137..9811)
                     /locus_tag="VN377_03075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR27403.1"
                     /translation="MVEAMEISRVTRTRTEAKTFYNKISPWYTLLAGASERRCWDACL
                     QKLHIKEGEQVLEIGFGTGTCLLELAGSIGDSGKIYGIDISEGMCEITQQKVNKAGLN
                     KRVNLRCVDAIPLLFDTSIFDAIIMNFTLELFDTPEIPQLLSECRRVLRPHGRIGVVS
                     LSKKQGSNAMVQMYEVAHKWFPRIIDCRPIFIEEVVTTAGFQVEEAMEMKLWGLPVIA
                     LVATRK"
     gene            complement(9818..10240)
                     /locus_tag="VN377_03080"
     CDS             complement(9818..10240)
                     /locus_tag="VN377_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWR27404.1"
                     /translation="MKAVVKQIDSCSFIGKADSNHWVSIDTKKEVSGSDAAAHPMELV
                     LLALGSCTGCDVISILQKKKVPLQDFEIHIDAERAEEHPRVFTKIHLEFVFVGTGLNP
                     TPLERAIELSQQKYCPVSAMLRPSVPITTSYRIVEKQQ"
     gene            10351..11688
                     /locus_tag="VN377_03085"
     CDS             10351..11688
                     /locus_tag="VN377_03085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWR27405.1"
                     /translation="MKKNDLKKFLSLLLCCLCTVLFLFPYASGSLLDLRMIEPKITAA
                     KGGISDLSEMFAQVNESHLRSYVEDIQAFGPHPTESQALTDLRDYLYSQFSAMNLPVQ
                     LDPWVEKQRSGENIVATLEGTLSKDVTVILCAHYDSLAISPGAEDDGSGVAAVLMIAD
                     IMRQYSFNTTVQFILFSGEEQGLLGSRSYATNLSAHHQNILGVLALDKIGYAITSEEG
                     NSVRHHANPASAWMSVLSQSVASDYPDDIGLVVLSLPQDPDSDHFSFVEQGYAGTDFV
                     RNATNPFYHTSEDIAAHMNFSYLTKVCKLALGTVASMACLHPRLSNHDLVITMKGTRL
                     STSAQFFVLVENSNETADSANVTIEITLKQLFRKEYVSTIKQGVYTPCNWTVVKEIVQ
                     SWEFAVAGRKFTRGFFTITVTLLGGDDDLYLYVTRQCLGVIMSPYRVVIIPRL"
     gene            11913..12221
                     /locus_tag="VN377_03090"
     CDS             11913..12221
                     /locus_tag="VN377_03090"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HWR27406.1"
                     /translation="MYLYLQLLCIFVIAPNIALLYLNRKKAHLKSLGIALVILFLIAL
                     IWDQLSVRMGLWIFSQDEIVGNLFGLPVEEYLFFLFVPLLSINIYLFVEGILSKSKKR
                     "
     gene            12585..13058
                     /locus_tag="VN377_03095"
     CDS             12585..13058
                     /locus_tag="VN377_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWR27407.1"
                     /translation="MRRAEKEITNRREIDSILSKATVCRIGFIDHETPYIVPLNFGYS
                     DNNLYFHSAQDGKKIELLKRNPLVCFEVECDLEIIDTGIPCKWSMTYASVIGHGTASF
                     ITDIKQKQNALNIIIDHYSPGSSYIFPEKNLKEVTIVKIEINQMTGKKSQPKKIQ"
     gene            13144..14325
                     /locus_tag="VN377_03100"
     CDS             13144..14325
                     /locus_tag="VN377_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR27408.1"
                     /translation="MVGIDTTRSTKYFLFGSLYFSEGLMIAITTVATSLYLREKAVSI
                     PITTLIVGIINIPWMLKFVWGPVIDYFIRFGRKTFIILGGILTVSCMFLISFLDPSVS
                     LVPFTVLMFLSHVGIGFIDVSADAWAIDVSTDKDRGKINGAMYAGQYSAWAIGAALFP
                     FLGSQYGYHSVYLINALLILLILLFPFIVRETVKRKIKEKIASLVVKEFKTRTVLLVA
                     LFSPLVFMNEGIMSFIIPIYMRDGLGLSDVQIGFIAMILPVFLTVGSLFGGILTDKIG
                     RKSALYLFIVLNIVFTASLIFADSWWKLSIIYGVIGFLMGGHSTVSCALFMDVTNPRI
                     GATQYGILTGIANVGLNGAGMVTGSLVVILGFARTFLYAAWAFGPALLVLYFIRIKKG
                     P"
     gene            complement(14408..>15954)
                     /locus_tag="VN377_03105"
     CDS             complement(14408..>15954)
                     /locus_tag="VN377_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27409.1"
                     /translation="KILSLLMISIFLLSLNVSAVKTSQTSTNDKTITDSTVQSTQNAP
                     YGEEGDIDPLVDLEVTVTIKEIRALDKIDDIGKADFFVIVYINDVKFKSKIWFNQNYI
                     DEPWSATLNVPDEEENVSIKIQLWDWNLGLSRICDLSDNSALLLSNYDIDLVYNLKTA
                     HWTGDDYNYPYMSWADPSGYGRLNGCDDNSIYQEERDCLVLFDITQNDFDMDNIPYWT
                     EVNRYHTDPQVNDMGRDDDGDGVPIEWEFKWGTFIDYDYHDQTWNFEWFYDPFVWEDH
                     SHLDPDNDGLSNVEEYLTSQWGSDPFRKDIFVELDQMEAGPNGEQASVFPELGKDILR
                     DAFDKYNIHLHLDDGSMGGGEWIPFDNETPREELITLYQDYFLHGDNNNWRKGVFHYG
                     LLVYDATYAGYVFGVDDNYWGAYQISSKRVDKRSHGAGKAIGYASVYMHELGHTLNIK
                     MPGGHDAKSGFPWNINWWKWRPYKSCMNYGYTYLLVDYSDGSRGRNDHDDWSTLDLPG
                     FQLDIE"
BASE COUNT         4490 a         3485 c         3258 g         4721 t
ORIGIN      
        1 atcttgaatg gagcccacga tcagatctca gtacacttgt agaagaaatt actgttaaat
       61 aacagaggcg ggatacttgg ttattggtgc gatagggaat gaagaaattc cgcagtgtaa
      121 aactaaaaat tttttttccg cttcttgtca ttcttctcgt tgtttttcta acaagctctt
      181 tggtcattat tgaacgagaa tcaaaggccg caaaagactc gcttatcaat actgccacat
      241 ctttttcaag cctctcaact gccgctgtca tcgagaactt cgtattctat aatgaatctg
      301 gtttttacaa attcactgaa gtcatcgata atgtcatgaa actgaacgat aaccttatta
      361 ccatacaggt cgtcaatgtg aatggcaaga ttttatttga ctccgttgaa atacagaccg
      421 ggaaatacga tgaacgaaca aatggggaac gatttctcga aaatgcaact ctgatacaac
      481 gagcaggaga atcggtctat tccctttttc tcaatgaaca aacgagacgt gttgatatta
      541 ttcaaccatt cttcgaggac tggggaagac acgattattc agtgcgttat accttttcgc
      601 tcgcaagcct tgacaccatg accaatgata tgattgtgac gatatccctg tactcaggta
      661 ttttcatcat cgtatccttt ctgctgatat ttttcctttt taattacttc atatcgtcac
      721 ctatcgggga actgacaaag ggcgttcgca gaatggggaa aggaacactc ggatacgaag
      781 tcacaatcaa atcaaaggat gagatcgggg agcttgcaac tgcgtttaat acgatgagta
      841 ctgacctgaa aatctcaagg gatcatttag aagaatatag caagaatttg gaaaaactgg
      901 tcgctgaacg aacgaaagaa ttagaggaaa aaaccagacg tcttgagcag ataaatcaag
      961 atttaatagt cgcgcgagaa gagctcagtg tgttaaataa gaatctcgag ggaagagtac
     1021 aagaacgaac cggggaagta gaaaaattac tcaaacaaaa agatgagttc atcaatcaac
     1081 ttggacatga tttgaaaaac ccgttaggtc cactgataaa tctaattcct ctgcttgaag
     1141 aaaaagaagc agatcccgct aaaaaagaga tgcttaccgt ccttcatcga aatgctgatt
     1201 acatgaaaaa cctcgtggtg aaaacacttg aacttgccgt tttaaactca ccgaatacaa
     1261 ggttctctat agaggtactc aacttattca aagaggtcaa tcagattatt agtaataaaa
     1321 aaatcctgtt tgatgataac aatgttaaga ttttaaacaa cattggcgac tctttattga
     1381 ttaaagcaga tcgattacga cttgaggagt tgctgacgaa catctttgaa aactcggtaa
     1441 aatactgcca ggaagattgc aggataacgc tcgatgcaaa actgacaaat gattttatta
     1501 cgatatccat caaggataac ggtatcggta tgaacaggga tgagattgac cgtatcttcg
     1561 atgagttcta taaagcagat tcagccagac atgatattca aaacacaggt ctagggatgt
     1621 cgatatgcaa acgtattgtt gaaaagcatg gcggaaacat ttgggtagaa agccctggcc
     1681 ctggaaaggg taccaccgtg ttcttcacgt ttccggcagc attgacacca taaaaacatt
     1741 aattctgatt tcttacggga tttatggtat tcgatcttct ccctctgtaa caataattat
     1801 taaagacttc tttgatatat catgagaggc aagaaacatc atgaagaaaa tcatgctagt
     1861 ggatgatgaa aaggatcaga tctatttcat caaaacatct ttcgagaacc tcttcgggaa
     1921 agagtacacg attatccctg tggaaagcgg tgaaaaatgt tttgagttat tggagaatca
     1981 aggactccca catcttattc tcctggatat catgatgcca aggatgaatg gatgggaggt
     2041 gtttgatcgg atacgtgcaa acccctcctg gaagaacatc cctatcattt tcctcaccgc
     2101 tcgaaccgat gaatttgctg agcatgctgg cgtacttatc gcagaggatt acatcaaaaa
     2161 acccattgag atcaaagagt taaaagcccg catcgataat gtactgaaga aagtaaagaa
     2221 acaatagcac aaaattttct ctcgattctt tcttcgaaaa ccttgccttc ttcgatgaag
     2281 gaccttatac tttttcaggg agaatatttg atttgaaaac ctcagtgaca tacagataat
     2341 cgtaatttgc gatatcatct gggaattttt tccgaaggtc ttccatgacc cgataaaaat
     2401 gctcaaagtt cgtcgcgtat aacgtgattt ccaaatcata cccgccaatc gctttgtgga
     2461 tctcccagag aaatgggtta tcgctaaggt aggtgatgat ctggtttttc ttgttataat
     2521 cacgcaaaga taaattcacg atgaactcgc gtaacccaag tttattaatg tccatcgtga
     2581 tagaatagcg ttggatcacc cctgatttga tgagtttcgt aatccgggaa tggatcgtca
     2641 ccgttgatac tttgatctgg cttgcgatgt cgacactatt catcctagtg ttcttcgcca
     2701 gtaaatttag tatcttgtag tcgaccaaat caacatcaac ggtttgtcca ttatcccgat
     2761 attcatatgc acgttcatgg cttgtcgtta gttcattgag caacagggaa tgtttgtatc
     2821 cgaatgattc gtacagctgg gaaaaaaata tttctttgaa ataataccga tagtgtcgta
     2881 aggtgtcttc atagaacgca taaaaataat tcggggattt caccaaaata gtaatgatta
     2941 gatcataccg tcccttcgtc gaggcgatgc tccatgtgtt attatggttg acgaaatgag
     3001 agatgatttc cttttcttta tctggcgatg catactgtaa gacgatatag agtcgataga
     3061 ctgaaaaccc aagtttgaag acgtcgacca ttgcattgaa attttttatg atgccttttg
     3121 tctgcagtcg tttgatgcga tattccacaa cgcttttttg aagaccgacc ttttttgcaa
     3181 ccgatgaaag cgattgtctc gagttcaaaa agagatggta taagattttg tgatccttag
     3241 aatctaattc tcgcacaata aaaatcatcg tctatagggc taataaattt ttccaatctt
     3301 tactaccttg agaaaaacag cataaaacga acaatatggt tttatggacg ggattcatga
     3361 tggttctgtg gttttttcct tctcttgttt aaaatatttt ccacaccttc aattcatcgg
     3421 tagtgctttt atcattttta taagcgatta cttttatcat atgagcgccg aaggatcgtt
     3481 gactccacaa ccaaccatac ggtgccatgg tatctatttc ttgtagttca tcatcgatat
     3541 agaactccac gcgatcgacc aaagatttat tgacctcatg aaccgatgct tgaacctcga
     3601 tcgaaccgac caggatcgga acaaagaaag gaagtatttt tgtgtttgcg acatacagag
     3661 cattctccgg tttcgtgatg gtgacagtga ataatggttc ttggctgatt aataacgttt
     3721 gagcccctcc tgtcggtaga gaatcaagcg tgtaggtgtc tgaattttct ttcagaagct
     3781 cccctgattc cgtataccag gacgcggaaa acggcagatg caagacaata ttctgcactt
     3841 gatggtcata gacattggtg accacacagg tgatgatatc ttgttgtgag ctacaggctg
     3901 cgcgaacgta ttgccatgtc tcgagtccga ccggtgaata cgctgtgttc catcggtcat
     3961 agcgatgaag cagccaatca ccgcctcctg taccgacatc gaacgcgtac atgttccagc
     4021 catctgaccc gcacagcatc atcccatcga ctatcgaagg aggtgcggtg atgctggcac
     4081 caatctggta acgccagaga accgaaccgg tggtttcgtt caacgcataa aacgcaccat
     4141 caggaccatc ccgtgtgccg ataaaaagaa gcccgccact taacgtcggt gagctgatat
     4201 cacagcaacc cccgatcttt gtctcccaga gcagatgacc ggtgctggca ttcaacgcat
     4261 agacataccc gagggtatct gcgatgaaca catgcccatt atgatatgcg ttaaacgata
     4321 agggtccccc atggagcgaa tacgtccaga gtaactggcc gttctgttcg ctgtatgcat
     4381 tcatcgtact actgtaatag atagctaaaa agatacgtcc aaggccatcc gctgtgatag
     4441 aattatccca aggacccgcg gataaggaga tactccatat ctcctgacca gtggttgcat
     4501 tgattttgaa taattttcct acattatccc aaaacgcgcc gtagaccgcg ttatccttca
     4561 gcatcggttt attcgcacag acagagaaac ctgtctcata cgtccagttg atgctcccat
     4621 cgtttttgtt taacgcatac atttttgtct cataaaaacc agaacaaaaa tagatcttcg
     4681 tttcatcata ttggactgac tcagcgatac caccgtagat cggtgtcttc catacttgtt
     4741 ccccggtgaa tctgtcaaaa gcgtacatat attcattctc agggtcatat ccatgggttg
     4801 aactgccgat gtacacgacg tctccgtcga tgaccggacc gacatagcct ggtcctgtgt
     4861 cattgctcca gatgatatcc cctgtggtcg tgtccactgc aaagattttg tcatgaccag
     4921 ctccgtgagt gctgcaggaa ccaatgtatg cgataccatc ggtataggtc accgcagatt
     4981 cctcgtagtt ggtgcctgtt gtatttgctt tccagagcag ttcatgaccg tacgcgacac
     5041 ttgagatgtt aatctcaatg gccacttctt tttcaaaatc aaaaaccatt ggaagttgtg
     5101 gatctgttac tgttctttga gactcaatgg ggaatgcatc agcacctata gcggatactc
     5161 caaggagtat aatcaagaga atgcttagta tgtttttcat ggaattttcc tcttgtgtca
     5221 catcaagatt caacgagatg gactttatat ctattggacc tatatattat ttatctattc
     5281 gtatataggg tcatccaaag attattaata tccttcgctt tataaatata gatgatgaaa
     5341 acgttcaccg ctgtcttcct tggttttttt cttctcattt ctaccttgta catcccaggt
     5401 attggatggg agaccgctag ttgtttatca ttagaacctt cttcaaatag atccttacga
     5461 ttatcttccg tagcagatgc tgtggttgtt tctgagagcg atccttattt tagtctcatc
     5521 ggcacaggtc ttgcctgttg gtatgacaaa gatacgaatt ctacagggct tcttccgtta
     5581 ttggtccacc atgagggtaa tctcaccgat gcccaaacac ggtttcttga aacctttttc
     5641 gactctacaa atcagacact tttggttctg ggtgaacatc tgaagacttc ctatgaatca
     5701 attgagattt tggggtccgc gccatcggtc gcgatttccc tagcaacttc tgtgtttacc
     5761 accgcatcag atgttctgat catcccgtat ggtacgttgg atgcgtatca gctcagtctc
     5821 cttgccaccc ctctcgctag ttacctggac atgcctttac ttatctttga tgataatgct
     5881 gtggagattc agatagcctg cacgcagctt cagacaaccc atgcatatgt cgttggggat
     5941 attactcttc atcttgagaa tatcaccgtg acatccctta ctaatgaaac agcgatcact
     6001 gatatgattt tgactgtcat caaagatcaa ttcggttcga ttaattatct tactatgacg
     6061 aacccctcag acgttgtccc cccagcggtt ctcaacatca cgcacctgtc attctctgac
     6121 catgtcatca ataaaaaaat aatcatcctg ggtaaggagt tcgatattgt tgggaatgat
     6181 acccgtaact atacgatttt tgtccccgat ggtcttactc gtatccagat ttctggggaa
     6241 atttttcaaa aacatggatc gttgcttgag cgtttctcct cgattgttcc tcttcttttc
     6301 atgaccctga ctgatgctca ggggcggatt gttgcgtatg cgaatagcat ggggtatgac
     6361 attggaaaga gctatgtcga gactcttagc tgtaacgcat cagggatcta tactcttcat
     6421 atacgagtgt acaacgggat aaaaggtggt ttttttgttc agagggggat ctccttcgtc
     6481 gatgccaata tcacgatgat ggcgactctc tcagcattga gttctcctca tcttccgctc
     6541 atcccaaagc tttccagtct tgcaccgtac cttgctgcag cccatggggg gctcatcatt
     6601 gcgaatgtgt cctgggagct taccgatgag tcgtattcat ctgctgctca aggatccggt
     6661 gcaggtcctt ggtacaatga gtctctacat cactttacga acacgaaggt caacatgaca
     6721 gtccagcaac taacgagaac attagaaaca cttggtaccc atgatcttct caacggatat
     6781 ctcagtgggc cagcttggct gactctcctc gcggatacga cgatgattcc gatgtactac
     6841 tacggtcctt ctcaggagga tatccctgac cgagggttgc catcggataa cccgtatacg
     6901 ttaaatcaga gtctatcggt gggacgtctg atcagttggg atgttcagga tagcagtgtg
     6961 ttaatcgctc ggacgttctt ctatgaagct ctctgcggtg aaccaggaag ttcagacgat
     7021 tggcattctc gattccattt cgttttcggg gagggattta gtgagactgg tggggttttc
     7081 catcagatcc cctatgcaaa agaaatcagg gagtatgggt ttacttcgaa ggtttatggg
     7141 gactttcgga acagtcggca gatcgcagag atccttggga tcttcactgg tgcgaattac
     7201 cttgaatatc tgggtcatgg ggattggttt tggtttcccg cgtcactcta tgggttggat
     7261 agctattcga aagcgtttga tgtcgctcat gtgaagaact gggtgtttga gaaaccaagt
     7321 ctcttcctct cgtcagcgtg tttgatgggg cgtactgatg gtcttccccc gcagatgaac
     7381 attgggctta ccatgcttca tgctggttgt aatggtttta tcggtgcgac aagagaaacc
     7441 ggtcaagagt ctggtctgac ggttcttgaa aatcatttga tcgtggatga ctggagtgtc
     7501 ggtgaggcgt tacgcgggga aaaacgtatt gatgcagaac ttccaacgtt ctatgttcgc
     7561 gtattgtacg gtgatcctgc atttaaccct tatgagccac agcatggttt cagtaatcaa
     7621 ggaagaccga tattactggg aagataacga gagttgcttg ggcgatggat ggaatgatgg
     7681 acgtaaaatt gagaaaggtt ttaagctggt gaggcaagga ggaaaagatg gataaaaaaa
     7741 actacactcc ctcatatttt acgtccctac ttaacaattg ttaattccga tttatatcac
     7801 tttcgctttt tcccagcttt ttcgaagaaa aaacagtgga aatacgtatc agaatacaca
     7861 aatggcacga ggaaaacagc caaataaaaa gcactgatca gtgatttttc agagaacaaa
     7921 atacgttttt ttttcgataa tattttttct ctctcgggta acaaacattt ataactcatt
     7981 tccctatcct gaagcgacgt gatacattat ggtttcagca gaagatattc ttataaaaga
     8041 ggtagtgaaa cgagattcaa acgataattt ctcccaggcc attaaccttt tcgacaaaac
     8101 tgacgccatc attattatgg ataataaaaa gtatcaaggg atgctcctga aacgaacact
     8161 catggaaccg accgtaaccc tccagacaaa attgcacacc atgctgacac atcctcccaa
     8221 actcacccca acggcatctc tggaagaaat cgcctgcgcc atggttgaaa acaacatctc
     8281 tacccttccg gttcttcaca atgacacctt acttggcata gtcaccgcag atgacgttat
     8341 caaaaaactc actgagcaag ccatcggggc acaaccagtg aaaaccatca tgtgtaccac
     8401 acctctttcc attagtccta cggaaacaat tgggaaagtc cttcatctgt tccgacaagg
     8461 acattctccc tatattgcgg tagttgatca ttcgaccgtc gtcggtgcta tcaccattaa
     8521 caccatcatt aacaaagtcc tccatagtca tagcaacgtc gaggggcgag ataacttcga
     8581 atcagagaaa agacaaaaac ttgatttacc cgcaaaatcc ttcatgaatg aacaaccctt
     8641 acttttgtca ccagaaacct ccatccgtga tgttcatagc aagatgcatc aattcgagca
     8701 gcctggcatc ctcatcggga aagataatca tctccatggt atcgtcaccc ataaagagtt
     8761 ccttgctccg ttcgctgaaa tcgaaaaaga agaaaccatc gccattcagt tccatcaccg
     8821 taacagcaaa gtcgaagggt tcgataaaga acaagcagta catttcctcc atgcggaatt
     8881 ccttaaaaac tatgaaaaat atcttgaggt cggctacctt catgtctctc ttgaacagca
     8941 caaagaaatc aaacaaggac ttttccgagt cgtctgtgaa atgaagcttt caggaaaacc
     9001 tggtgttttt tatgcgacac aggaagggta cggcccccag caggcgatga ggaatgcgtt
     9061 tctcgccatt gaacatcaaa tcaataaagt aaaaaaaacg taggtacgaa cacttgatat
     9121 atttcatata tttcttttat tttcttgtcg ccaccagtgc tatcacgggc aatccccaaa
     9181 gcttcatctc cattgcttct tcgacttgaa aacctgcggt ggtaaccacc tcttcaatga
     9241 aaattggtcg acaatcgatg atccgaggaa accacttatg tgcgacttca tacatttgta
     9301 ccatcgcgtt cgatccttgt ttttttgata acgataccac cccgattcgt ccatgcgggc
     9361 ggagaacgcg acgacattca gagagcagtt gtggaatttc aggggtatca aaaagctcga
     9421 gagtaaaatt cattattatc gcatcaaata tactcgtatc gaagaggagc ggtatcgcat
     9481 caacgcatcg gagattaacg cgtttgttca gtccagcttt gtttactttc tgttgtgtaa
     9541 tttcgcacat tccttcagag atatcaattc cgtagatttt gccggaatca ccaatcgatc
     9601 cggctaattc gaggaggcat gttcctgtcc caaatccgat ctcaaggacc tgttctcctt
     9661 ctttaatatg aagtttttgc agacatgcat cccagcatct ccgttcagac gcccctgcta
     9721 gaagagtgta ccaggggctt attttattgt agaacgtttt tgcttctgtt cgtgtccgtg
     9781 ttactctgct gatttccatg gcttcaacca agacttgtca ttgctgtttt tccacaattc
     9841 gataggatgt ggtgatagga accgatggtc tgagcatcgc tgatactggg caatatttct
     9901 gctgtgatag ctcaatggcg cgttcgagag gtgtcggatt tagtcctgta ccaacgaaga
     9961 cgaactcaag atgaattttc gtaaacaccc gtgggtgttc ctctgctcgt tccgcatcga
    10021 tatggatctc aaagtcttgt aacggcactt ttttcttttg tagaatcgat atcacgtcgc
    10081 aaccggtgca actccctaat gctagcagca ctagctccat aggatgtgcc gcagcatcag
    10141 atcctgacac ttctttcttc gtgtcgattg atacccaatg attgctgtct gcctttccga
    10201 taaaagaaca gctatctatt tgttttacaa ccgctttcat ggcccctcac gagattacta
    10261 tagatatggt tctgtagtct ttatcgttta tccttgcctc attctagagg ccatacattt
    10321 aaataaacag attaatatgt tttttaattg atgaaaaaga atgatttgaa aaaatttctt
    10381 agtcttctcc tctgttgtct ttgcactgtc ctttttctct ttccatatgc ttctggttct
    10441 ctcttagatc tgaggatgat cgaaccgaag attaccgcag cgaaaggagg aatatctgat
    10501 ctttctgaaa tgttcgcaca ggtcaacgaa tcacacttac gatcctatgt agaggacatc
    10561 caagcgtttg gtccgcatcc tacagaatca caagcactga cagatctccg agattatctg
    10621 tatagtcaat tctcagcgat gaatctgcca gtacaattag atccatgggt ggaaaagcaa
    10681 cgatctgggg aaaacatcgt ggcaacattg gaaggcacgc tctcaaaaga tgttacggtc
    10741 atactctgtg ctcattacga cagccttgcg atctctcctg gtgctgaaga tgatggttca
    10801 ggggttgcgg cggttctcat gatcgcagat atcatgcgcc agtactcgtt taataccacc
    10861 gttcaattca tcttgttttc tggtgaagaa caaggtcttc tcggcagtcg gagttatgca
    10921 acaaacctct ctgcgcatca ccagaacatc cttggagttc ttgctcttga taagattggt
    10981 tatgcgatca caagcgagga aggaaacagt gtgaggcatc atgcaaaccc tgcgtcagca
    11041 tggatgagtg tgctaagtca atctgtcgcc tctgactatc ctgatgacat cggtctggtc
    11101 gttctctctt tgccccagga cccggatagc gaccatttct cctttgttga acaaggatat
    11161 gctgggactg attttgtcag aaatgcgacc aacccgttct atcacacatc ggaagatatc
    11221 gctgcccata tgaatttctc atatttgacc aaggtctgta agcttgcttt aggcaccgtt
    11281 gcatcaatgg cgtgtctgca tcctcgattg tcaaaccatg accttgtcat tacaatgaaa
    11341 ggaacccgat tatcgacttc agctcaattc tttgtgttgg ttgaaaacag taatgagact
    11401 gccgattctg caaatgtcac tattgaaatt acattaaagc agctcttccg aaaagaatat
    11461 gtatccacta tcaaacaagg agtgtatacc ccctgcaatt ggacagttgt aaaagaaatc
    11521 gttcagtcgt gggaattcgc tgtcgcaggt cggaagttta cccgaggctt tttcactatt
    11581 actgtgacac tgttaggcgg agatgatgat ttgtatctct atgtcactcg acaatgtttg
    11641 ggagtcatca tgagtccgta ccgagtagta atcattcccc gtctgtaaaa tttttttcta
    11701 atacgacgaa agccattggg aagtagaatt actgatgagg gggaggaaag gttgaaacga
    11761 aaaaaatctc atcagcaact gtacctctct atattaacaa ttgttaattc ttatttttat
    11821 actttttggt cacctataaa aaccaatcat tacaaccgaa ggtttttgtt ttcatagatg
    11881 aatatttata gcatgttccc attcattttt ccatgtactt ataccttcaa cttctctgta
    11941 ttttcgtcat tgcccccaat atcgctcttc tctatctgaa tcgaaaaaaa gcacatctga
    12001 aatcactcgg tatcgccctg gtaattttat ttctcattgc gttgatatgg gatcaactct
    12061 ccgtccgaat gggactatgg atcttctccc aagatgagat agtcggaaac ctttttggac
    12121 tgccggtaga ggaatacttg tttttccttt tcgtcccgct tctctccatc aatatctatc
    12181 ttttcgtaga aggaattctt tcgaaatcaa aaaaaaggtg aacaaagatg aatgaaaacg
    12241 aattaaaaaa caaaagaaat caaatggcat gtcaaaaatg caaaacagac atcggcggaa
    12301 aaccatacta tgtggttcaa ctcgattgta tctatgacgg gaattccctt tctgcctgcg
    12361 ataaaatcgt tgtttgctct gcttgttttc gtaatcttaa gtcttggtta aatctttctt
    12421 aaaattacgt tgagcaggat atgaatacta ttataggaag taaattgttg atgaggattt
    12481 gggaggaaag aataaaaaag atacgctcat caacaattac acctctctgc attaacaatt
    12541 gttaataccc tatataaatg tttcgttcta aggagaacga gcacatgaga cgagcagaaa
    12601 aagaaatcac caacaggaga gaaatcgatt ccatactctc aaaagcaacc gtctgcagaa
    12661 tcggcttcat cgaccatgag accccctaca tcgtcccatt gaattttggg tatagcgaca
    12721 acaatctgta tttccattca gcacaagacg ggaaaaaaat cgagttacta aaaagaaatc
    12781 ccctggtctg ttttgaggtc gaatgtgacc ttgaaatcat cgacactggg atcccttgca
    12841 aatggtcgat gacctatgca agcgtgatcg gacatggaac cgcatctttt atcacagata
    12901 tcaaacaaaa acaaaacgca ttgaacatca tcatcgacca ttactcacca ggatcatcgt
    12961 acatttttcc agaaaaaaat ctcaaagaag tcactatcgt caaaattgaa atcaatcaga
    13021 tgaccggaaa aaaatcacag cccaaaaaaa tccaatgacc atcaattgga agcatggtag
    13081 cgtaatgaga ggaatttgta tccatacaaa tatttaagag attatattaa tatatattta
    13141 tgaatggttg gtatcgatac tacaagaagt acaaaatatt tcttgttcgg cagcctctat
    13201 ttctcagaag ggttaatgat agcgataaca acggttgcca cctcccttta cctcagagaa
    13261 aaagcagtat caataccgat cactacactc atcgttggaa tcatcaacat cccttggatg
    13321 ctcaaattcg tctggggtcc ggtcattgat tattttatca gattcggacg taaaaccttc
    13381 ataatactcg gaggaatact caccgtttct tgtatgtttc tcatctcatt cctcgaccca
    13441 agtgtttccc tggttccttt tactgttctg atgtttctta gccatgtcgg catcggtttc
    13501 atcgatgtgt cagcggacgc ctgggctatt gatgtatcga cagacaaaga ccgtgggaaa
    13561 atcaatggtg cgatgtatgc tggccaatac tcagcctggg ctatcggtgc cgctcttttt
    13621 cccttcctag gctcacagta cgggtatcat tccgtctacc tcatcaacgc actgctgatt
    13681 cttcttattc ttctgttccc ttttatcgtc agagaaacag tgaagagaaa aatcaaagaa
    13741 aaaattgcgt ctctcgtcgt aaaagaattt aagacaagga cggttctgct ggtcgcgctt
    13801 ttttctccgc ttgtctttat gaatgaaggg atcatgtctt ttatcatccc catctacatg
    13861 agggatggac ttggtctgag cgatgtccag attggtttta tcgccatgat cctgcctgtg
    13921 tttcttacag tcggctctct ttttggaggc atcctgaccg ataagatcgg taggaaatca
    13981 gcactgtatc ttttcatcgt gttaaacatt gtgtttaccg cttcccttat ttttgcagat
    14041 tcatggtgga aactctccat catttacggc gtcattgggt ttttgatggg aggccattcg
    14101 acagtatcat gcgcattgtt catggatgtc acaaatccaa ggattggcgc gacgcaatat
    14161 gggatcttga ctggtatagc gaatgttggg ttgaatggcg ctgggatggt cactggttct
    14221 ttagtggtca tccttggttt tgcccgtacg ttcctgtatg ctgcctgggc gttcggtccg
    14281 gcgttgcttg tgttgtattt catccgaatc aaaaaaggac catagagttt tttaaaaaaa
    14341 ttctgaaaca actttacata cagaccacgt gggtgatacg tagaacaacg cttggttgga
    14401 atcttgttca ctcgatatct agttgaaatc caggtagatc aagagtactc caatcatcat
    14461 gatcattcct gccgcgagag ccatcggaat aatcaaccag gagatatgtg tacccgtaat
    14521 tcatgcaact tttataggga cgccatttcc accagttaat gttccaggga aacccgctct
    14581 ttgcatcatg cccccctggc attttgatat taagcgtatg gccaagctca tgcatataga
    14641 cgcttgcata gccgatagcc ttgcctgcgc catggctacg tttatcgact cgtttacttg
    14701 aaatctgata tgcaccccag tagttatcat ccacaccaaa aacatatcct gcataggttg
    14761 cgtcatacac gagtaaccca tagtggaaca cgccttttct ccagttgttg ttgtcaccat
    14821 gaaggaagta atcttgatac agtgtgatta attcctcgcg aggtgtttca ttgtcaaaag
    14881 gtatccattc tccaccaccc attgatccat catccagatg cagatggata ttatacttat
    14941 caaaagcatc tctgagtata tccttgccta attcaggaaa tacactggct tgttccccgt
    15001 ttggacctgc ttccatctgg tcaagttcca caaaaatatc ttttctaaag gggtctgatc
    15061 cccattgcga tgtcagatat tcttcaacat tactcagtcc atcattatca ggatccagat
    15121 gagaatggtc ctcccacaca aatggatcat agaaccattc aaaattccat gtttgatcat
    15181 gataatcata atcaataaaa gttccccatt tgaattccca ttctattggg acgccatcac
    15241 catcatcatc cctccccatg tcattcactt gagggtctgt gtgataccta tttacttcag
    15301 tccaataggg aatattgtcc atgtcaaaat cattttgggt gatgtcgaac agtactaaac
    15361 aatctctctc ttcttgatag atgctgttat catcacatcc gtttaatctc ccgtacccgc
    15421 taggatcagc ccatgacata tacggatagt tgtagtcatc ccccgtccaa tgtgctgttt
    15481 ttaggttata gacaaggtcg atatcatagt tactcaataa taaagcacta ttatcgctaa
    15541 ggtcacaaat tctgctcaga cctagattcc aatcccataa ttggattttt atcgagacgt
    15601 tttcttcctc gtcaggcaca ttgagggtcg cggaccaggg ttcatctata taattttgat
    15661 taaaccatat tttactttta aatttcacat cgttgatata gacgataaca aaaaaatctg
    15721 ctttaccaat atcatcaatt ttgtctaagg ctcgtatttc ctttatcgtt actgttactt
    15781 ctagatcgac taatggatcg atatctcctt cttcaccata tggtgcgttt tgtgtagact
    15841 gaacagtgga atcagtaatc gttttatcat ttgtcgatgt ctgagatgtc ttgactgcag
    15901 agacgtttag agaaagtaaa aagatactta tcatgagcag gcttagtatt tttt
//
LOCUS       DATMXJ010000110         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_69805, whole genome shotgun sequence.
ACCESSION   DATMXJ010000110 DATMXJ010000000
VERSION     DATMXJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69805"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..721)
                     /locus_tag="VN377_03110"
     CDS             complement(398..721)
                     /locus_tag="VN377_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27410.1"
                     /translation="MKKHVALKDGTIVCIEPLTGGEDIREFQRFINTLTREGTYLLVN
                     KPITLKEEKQWFKTQVTEQRKGQQIYLKALVDGRLIGDCFAKPGFGRNHGNVNLGIAI
                     TKKWR"
     gene            882..1640
                     /gene="truA"
                     /locus_tag="VN377_03115"
     CDS             882..1640
                     /gene="truA"
                     /locus_tag="VN377_03115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWR27411.1"
                     /translation="MRVAMKFAYDGKNFSGYARQPGQRTIEQMVVDVLIEQGMFQDVK
                     LSCFRTASRTDRGVSALGNVCAFDTTLSKDDIMMLFQNDGSEVFFYGVQEVIPCFYPR
                     HATRREYRYYLRNHGLDVEKVLGAAGLFAGEHNFQNFARVEPLKNPVRTIDTIVISER
                     KDFLILDFYAQTFLWNQIRRIVSALKKVGNGTLSLKEVRHALDHPEKKVDFQVAAPEP
                     LLLKDVVYDFSFNYIKEYAKKLLRFESNIVSSLE"
     gene            complement(1659..2669)
                     /gene="pfkA"
                     /locus_tag="VN377_03120"
     CDS             complement(1659..2669)
                     /gene="pfkA"
                     /locus_tag="VN377_03120"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWR27412.1"
                     /translation="MMGQGKYIGVLSSGGDAPGMNAAIRAVVRTGIYHGNTMFGIKRG
                     YQGMIEGDIEQVNRYYVSNIIKDGGTVLQTARSSEFLTKEGREKAFKSLKRFGIDSIV
                     VIGGDGSFRGLHQLIEEFPIQGIGVPGTIDNDLYGTDYTIGFDTAVNTALEAIDKIRD
                     TASSHSRLFIIEVMGRTSGYIALYTGIGGGAEDILIPESPMQIPQLVEELAAQYKAGK
                     HSSIIVIAEGETTGGAAEIKTAIEKVINWDIKVSVLGHVQRGGDPSAFDRVLASRLGY
                     ESVLAVMNNETDKMVGMVNKTLALTPLQDTWTKKKDMDLTYLKMNEVLAAQQVKALEV
                     TV"
     gene            2821..2905
                     /locus_tag="VN377_03125"
     tRNA            2821..2905
                     /locus_tag="VN377_03125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2857..2859,aa:Leu,seq:caa)
     gene            2971..>3407
                     /locus_tag="VN377_03130"
     CDS             2971..>3407
                     /locus_tag="VN377_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27413.1"
                     /translation="MKKYPLIRKGFAVGIILLFVGTCIIPTIAQDNEKPLPTSRGNWS
                     YVGGSGPGNYTKIQDAINDSHDGDTVFVYSGLYTETIVIPTAIRLIGESKNTTRITAK
                     ILSGEALISLSSSNVTISGFTFYGIDSEYSLILRTIYHYGKLR"
BASE COUNT          816 a          709 c          797 g         1085 t
ORIGIN      
        1 gtcagtcagt caaaaaaaga tattcaactg atggacccga aaaactattc gacggttgag
       61 attcagaaac caaaaatgat ttcgatcgat acctcgatgg tgaaaacagt gaagttggac
      121 ggttaccttt ttttagtccc tgggtaagta ggttttttca ttttttttgt tgctgtaaaa
      181 aacgttcttt atcaaggcga aggagatact catcaagata tccattgtgg tactcaaacc
      241 attgtggaag ctgtgcgatg atctgaaaac caagggattg gtagagctga taggcttttt
      301 tatttgctga gacgacatgc aggtaaatgt ttttcggatg ccatttcttc tctgagcgtt
      361 cgattagttc tgtaaggagg atatgaccta agccttttca tctccatttt tttgttattg
      421 ctatccccag gttgacgttc ccgtggttcc gaccaaagcc tggttttgca aaacaatccc
      481 cgatgagacg accgtctacg agtgctttca ggtagatttg ctgtcctttt ctttgttctg
      541 ttacttgtgt tttaaaccat tgtttttctt cctttaatgt tattggtttg ttgacgagaa
      601 gataggtgcc ttctctggtg agtgtgttga tgaaacgctg gaattctctg atatcttccc
      661 cacctgtgag cggttcgata catacgatgg tcccatcttt taacgcaacg tgttttttca
      721 ttgggtttct tgcctcattt caaggattgt tcatctttgt tttttcttcg gtatcttttc
      781 ttcctaggat gcaggtcctg tgtcatatcg tattggtttt tatagaacta tcttcttatg
      841 catcagaatg gttttatggg aggaatcctt gtggtatgtg tatgcgggtc gcaatgaaat
      901 ttgcgtatga tgggaagaat ttttctgggt atgccaggca gcctggtcag cggacgattg
      961 agcaaatggt tgtggatgta ttgattgaac aagggatgtt tcaggatgta aaattgtctt
     1021 gtttccgtac cgcgagtcgt accgatcgtg gtgtgtctgc gttggggaat gtctgtgcgt
     1081 ttgacacgac tttgtcgaaa gatgacatca tgatgttgtt tcaaaatgat ggatcagagg
     1141 tgttcttcta cggtgttcaa gaggtgatac cttgttttta tccgcgtcat gccactagaa
     1201 gggagtaccg gtattatctc cgaaaccatg gtcttgatgt tgagaaggta ctcggtgctg
     1261 ccgggttgtt tgccggggag cataactttc agaatttcgc tcgggtcgag ccgttgaaaa
     1321 acccggttcg gacgattgat actattgtca tctctgagag aaaggatttt ttgattcttg
     1381 atttctatgc acagacattc ctatggaatc agatccggcg gatcgtatca gcactgaaaa
     1441 aagtaggaaa cggtactctt tctttaaaag aagtcagaca cgcgttagac catccggaaa
     1501 aaaaggtgga ttttcaggtt gctgcacctg aaccgttgct cctgaaagac gtggtgtatg
     1561 atttttcttt taactatata aaggagtatg cgaagaagct tctgaggttt gagagcaata
     1621 ttgtctcttc tcttgagtga aggtttctgc agagatttct agactgtcac ttcaagtgct
     1681 tttacttgtt gggctgccag cacttcgttc attttcagat acgtcaggtc catgtctttt
     1741 ttcttcgtcc acgtgtcttg taacggtgtg agtgctagtg ttttattgac catccccacc
     1801 attttatcgg tttcattgtt catcaccgca aggaccgatt cgtagccaag tctgctggca
     1861 agcacgcggt cgaaggcaga ggggtctcca ccgcgttgca cgtgacctag gacactgact
     1921 ttgatgtccc aattgatgac tttttcaatc gctgttttta tctcggccgc tccaccagtt
     1981 gtttctcctt cggcgattac gatgatgctg ctgtgtttcc ctgctttata ttgggcggcc
     2041 agttcttcga caagttgtgg tatctgcatg ggtgactcag ggatgaggat atcctctgca
     2101 ccgccgccaa tgccggtgta gagtgcaatg taacctgagg tccgtcccat gacctcgatg
     2161 ataaataatc tcgaatgtga agatgcggta tcccgaatct tgtcaatggc ttccagggcg
     2221 gtgttgaccg cggtgtcaaa ccctatggta taatcagtcc catagaggtc attgtcaatc
     2281 gtcccaggga ctccaatgcc ctggatcggg aattcttcga tgagttgatg cagtccgcga
     2341 aacgaaccgt cccctccaat caccacaatg ctatcgatgc cgaaacgttt caggcttttg
     2401 aatgcttttt cccgtccttc tttggtgaga aactcactgc tccgagcggt ctgtaggact
     2461 gtgcccccgt cttttatgat attgctgaca tagtatcggt tgacttgttc gatgtccccc
     2521 tcgatcatcc cttggtatcc gcgtttgatt ccaaacatgg tgtttccatg ataaatcccg
     2581 gtgcgtacaa ccgcacggat agcggcattc atccctggtg catcaccacc gcttgagagt
     2641 actcctatgt attttccctg acccatcatc catcacctag tgctacactg aaatcgtttc
     2701 cagcttttaa aacattccgc ggaaaaagga ttttgaataa atgggttcat taattgttct
     2761 tttctctggc tgtctcaacc taaatattta ttatgaatga accgttcatg tgttgctcgt
     2821 gcaggggtag ccaagcctgg tctacggcga cagactcaag atctgttcct gcagaggttc
     2881 gggggttcaa atcccttccc ctgcactttt tttccctgac aaaggtttaa ataagcaagg
     2941 attgttcttt tctatagggg caggaaacag atgaaaaaat atcctttgat aaggaaaggt
     3001 tttgcggttg gaatcattct cttgttcgtt ggaacctgta tcattccaac gattgcacaa
     3061 gataatgaga aacctctacc gacatcaagg gggaattggt cgtatgttgg tgggagtgga
     3121 ccagggaact atacgaagat tcaagatgca ataaacgatt cacatgatgg agatacggta
     3181 tttgtctaca gcggactgta cactgaaaca attgttattc caacagccat ccgacttatt
     3241 ggagaatcaa aaaacacgac aagaataacc gctaaaattt tatccggtga ggctctcatc
     3301 tcactttcga gtagtaatgt tacaatctca gggttcacct tctacggaat agatagcgag
     3361 tatagtctta ttcttaggac catctaccat tatggaaagt taagaaa
//
LOCUS       DATMXJ010000111         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_39089, whole genome shotgun sequence.
ACCESSION   DATMXJ010000111 DATMXJ010000000
VERSION     DATMXJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39089"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..2939
                     /locus_tag="VN377_03135"
     CDS             63..2939
                     /locus_tag="VN377_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HWR27414.1"
                     /translation="MIDVLLGISPNYLYFQKKPPQPVSELHHPYFLAFPPTATDAKTA
                     RKKLLELWNQNKIDHPKLSKITGIGELETYRSFCDFTKTRNVFKVYTKESYVVPEISD
                     YLFFEHELFTAEHDIPYQQRALVDLAAEGKTWLFDTNGEKKNLKVLIYDIETTQFEEG
                     KPQIPIDIIGYACFDIAIESEKNLDTEEFSFEIRDAPAHWKDVEVLQHLSRSVDEEIA
                     NLLIFCKRAKDADIISGHNILGFDNVHVHDRINWVLKNKQEQLSIDEKKTFQNFSSLY
                     CREDKSFHFGIMGADIVQMYPCSFDTYLAARKFYPWLDDYRLKSLAPFLNIIVENRVY
                     LAPSQIKLDDRTMEYNKQDVQEQIGVSLHFLQQALPLSFTTCMPFDMLLSSGAVNMWD
                     HMALIRGAVQKKIVPPICRVKSISQMLARDFGSQATKEKIIRLAKQRREQLSKDLVRV
                     LKYGEEMPDWVQYPQVIYKEQVSADDDEEVVNYHMPGGMTIKPDKEAHSHFVPWYHVV
                     VADVGAMYPTILKAMNIGADTVRIATRNEKPDAWVWLKKLPEGFLKNRDVNWREITKE
                     DSYADKGYMIGVCIDTNPGVVNCAMTGIMSTIAKIKKELKEAKTKAHTSELESLKMMY
                     QSMKGARNAGTHGILAAPTVTGRQFNLWGAAAITTKGQMILADTMDQLHKQDIRVVYG
                     DTDGIYMGCSRSMGNLPGFSKALGVPLSPEDGKWITKPTVAYAAIKQCNEKWQKELKY
                     PDFELEAEHHDCMIFVKHKNYLIFDEKNGKIEMTTKGNNFKGSDKANIARKVLAEIMM
                     RVLQENPSWEKEEDARASVKESIKNITREIVSKLDLASVDLNDLTIVQSVQPEKRYKL
                     NQDGSSSTFGKRSEALEKLLGHRIRSRVKMKFVITKKPLPGIAKPSKSGVKPIDYMYP
                     IDLVKDRKEIDLDWYKKMIENYVQGAFGLSDIASTEQKGLDAWM"
     gene            3036..3197
                     /locus_tag="VN377_03140"
     CDS             3036..3197
                     /locus_tag="VN377_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27415.1"
                     /translation="MLIVGQVSSKEEANEITFIAKLFIVGQRARFLASNPQAACAADE
                     EDIEDEEEE"
     gene            complement(3247..3849)
                     /gene="tmk"
                     /locus_tag="VN377_03145"
     CDS             complement(3247..3849)
                     /gene="tmk"
                     /locus_tag="VN377_03145"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWR27416.1"
                     /translation="MKQFVTFEGIDGSGKSTISKLVSEKLRAAGYDTIWTFEPTDSTV
                     GKYVQECIRSKTDPYITSFTFIADRILHCKQIQQWLDEGKIVVCDRYAESTYAYQAAQ
                     LESELKNPLKWLQELSKDRILLPDRTYLFVIDPKISLARIQHRTELIPFERLAFLEKV
                     HRNYLKVSKGKRFLHLDATQPIDTLVQLCCNDILRGIDKK"
     gene            complement(3846..4880)
                     /locus_tag="VN377_03150"
     CDS             complement(3846..4880)
                     /locus_tag="VN377_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HWR27417.1"
                     /translation="MRELVTLAKYPFLHDTRQFIKDNGPSINELLHDPPYERARIISV
                     ERLDNALKNRDVGRRSLANESDCIMEILSYPLARMVAVCIGDSFFKKRYALGEASHAY
                     KYLLNESTPFLLTVAKELEVTVQPIPETNIIKIFFKDYLRNAPTRYKEWKMVNRGINR
                     GYLAITQKDLARILLEALRERINKELISRECDKSVFDVFSSDIQRFQNMLALQKKKIE
                     ATPVGKVSIEKLPPCMKDILSAIQSGENVPHMGRFSLVAFLSSLKLSTNDILKLFSTA
                     PDYQEDKTRYQVEHITGASSSTEYKCPGCEKMRTYGICPVDKMDELCKKIHHPLSYYS
                     NKWKQEKQKP"
     gene            4979..5052
                     /locus_tag="VN377_03155"
     tRNA            4979..5052
                     /locus_tag="VN377_03155"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5013..5015,aa:Ile,seq:cat)
BASE COUNT         1455 a         1019 c         1243 g         1459 t
ORIGIN      
        1 cctttaagta catccaatgt gttaagggta aaaaaaggac gattttgggg gaacatgagg
       61 gaatgattga cgtattgctt gggatatcac cgaattatct ctattttcag aaaaaaccac
      121 cacaaccggt ctccgagttg caccacccct attttctcgc gttccctccc acagcaaccg
      181 atgcaaaaac agctcggaaa aaactcctcg aattatggaa ccaaaacaaa attgatcatc
      241 cgaaactatc gaaaatcact ggtatcggag agcttgagac ctaccggagt ttttgtgatt
      301 ttacgaaaac aaggaatgtg ttcaaagtct atacaaaaga gtcctacgtg gtccctgaga
      361 tcagtgatta tttgtttttc gaacatgagt tattcaccgc tgaacatgat atcccgtacc
      421 aacaacgcgc tttagtggat cttgctgcag aaggaaaaac atggttgttt gatacaaatg
      481 gcgaaaagaa aaacctcaag gttctcatct atgatattga gacgacccag tttgaagaag
      541 gaaaaccgca gattcccatc gatatcatcg gatatgcttg ttttgatatt gctatcgagt
      601 cagagaagaa cctggacaca gaagagttca gctttgagat tcgcgacgct ccagcacact
      661 ggaaggatgt ggaggttctg caacatctct ctcgttctgt cgacgaggag atagcgaatc
      721 ttctgatttt ctgcaaacgt gcaaaagacg ctgatatcat ctcaggtcat aacatccttg
      781 gttttgataa cgtccatgtc cacgatcgga taaattgggt tttaaaaaat aaacaagaac
      841 agctttcgat tgatgagaaa aaaacatttc agaatttcag ttccttgtat tgccgggagg
      901 ataaatcatt ccattttggt atcatgggag ctgatatagt gcagatgtat ccttgtagtt
      961 ttgataccta tcttgctgca cggaagtttt acccatggct ggatgactac cggttaaaat
     1021 cattagcacc atttctgaat attattgtcg agaatcgggt gtatttagcc ccctctcaaa
     1081 tcaaacttga tgacaggaca atggaatata acaagcagga cgtccaagag cagatcgggg
     1141 tttcgttaca ttttctccaa caagccttac cgttgtcatt taccacctgt atgccgtttg
     1201 atatgttgct ttcatccggt gcggtgaaca tgtgggatca tatggcactg atccgcgggg
     1261 ctgtacagaa aaaaatagtc ccacccatct gtcgggtgaa atcgatctca caaatgttag
     1321 ccagagattt tgggagtcag gccacaaaag aaaaaatcat ccggctcgct aagcaacgac
     1381 gagagcagtt atcaaaggat cttgtccggg tgctgaaata cggtgaggag atgcctgact
     1441 gggtgcagta cccgcaggtg atctacaaag aacaggtgag cgctgatgat gacgaggagg
     1501 tcgtcaatta ccatatgccc ggtgggatga ccattaaacc agacaaagaa gcacattctc
     1561 atttcgtccc ctggtatcat gtagttgtcg ctgatgtcgg tgcgatgtat ccaacgatcc
     1621 ttaaggcgat gaacattggt gcagatacag ttcgaattgc gaccagaaac gaaaaaccgg
     1681 atgcctgggt atggctcaag aaacttcctg aagggttctt gaaaaacagg gatgtgaact
     1741 ggcgagaaat caccaaggag gactcctatg cagataaagg gtatatgatt ggtgtctgta
     1801 tcgatacgaa cccgggggtc gtcaactgtg cgatgaccgg tattatgagt actattgcga
     1861 agataaaaaa agaattaaaa gaagcaaaaa ccaaggcaca cacatcagag ctggagagtc
     1921 tgaagatgat gtatcaaagc atgaaaggcg cccggaacgc tgggactcat gggatcttgg
     1981 cggctcctac tgtgaccggg aggcagttca acctctgggg cgctgctgcg attaccacaa
     2041 aagggcagat gatccttgct gatacgatgg atcagcttca caaacaggat atccgtgtcg
     2101 tctatggtga taccgatgga atttacatgg gttgttcccg ctcgatgggg aacctccctg
     2161 ggttttccaa agcattaggt gttcctcttt cccctgaaga cgggaaatgg attaccaaac
     2221 ctacagtcgc atatgcagcg attaaacaat gcaacgaaaa atggcagaaa gagttgaaat
     2281 accctgattt tgagttagaa gcagagcacc atgattgcat gatttttgtc aagcataaaa
     2341 actatcttat ttttgatgag aagaacggaa aaatcgagat gacgacgaaa ggaaataact
     2401 tcaaagggtc agataaagcg aacatcgcac ggaaggtgtt agcagaaatc atgatgaggg
     2461 tgcttcagga gaacccgtcg tgggaaaaag aggaggatgc gcgtgcatcg gtcaaggaaa
     2521 gtatcaagaa catcaccaga gaaatcgtct cgaaactgga tcttgcgagt gttgatttga
     2581 atgatttgac cattgttcaa tcagttcaac cggagaaacg gtataagttg aaccaggatg
     2641 gttcttcatc gacttttgga aagcgatctg aggcgcttga gaaactactt ggtcatcgga
     2701 ttcggagcag agtgaagatg aagtttgtga taacaaaaaa accgctgccc ggtatcgcaa
     2761 aaccttcgaa aagcggggtg aaacctatag attacatgta tccgattgac cttgtgaagg
     2821 accgaaaaga aatcgatctt gactggtata agaagatgat cgagaactac gtgcagggtg
     2881 cattcggatt atctgatatt gcttcgacag aacagaaagg actggatgcc tggatgtagc
     2941 ggtattcttt tttttatcat taatagtact gatacctggt atcacttgta caacaaaccc
     3001 tttaaaagaa gaagacgaca ctatgtttga ggattatgtt aatcgtcgga caagtatcaa
     3061 gcaaagaaga ggcaaacgaa atcactttta tcgcaaagct ttttattgtt ggtcaacggg
     3121 cacggtttct tgcgtcaaat ccacaagctg cctgtgcagc agatgaagaa gatattgagg
     3181 acgaagaaga agaatagtga gggagttttt caggataata gagtgactgt tatcctttga
     3241 ttcttttcat tttttatcga ttcctcgaag tatatcattg caacagagtt ggactaatgt
     3301 atcaatcggt tgtgtcgcat ccagatggag aaaccgtttt ccttttgaaa ccttgagata
     3361 attcctatgg actttttcta aaaatgcaag tcgttcaaaa gggattaatt ctgtccgatg
     3421 ctggatgcgt gcgagggata ttttcggatc gatcacaaac aggtatgttc gatccggtag
     3481 aaggattcta tcttttgaaa gctcctggag ccattttaag gggtttttca attcagactc
     3541 caattgagct gcttgatagg cataggtcga ctcggcgtac cggtcgcata cgacgatttt
     3601 tccttcatct aaccattgtt ggatttgttt gcagtggagg atgcgatctg cgataaacgt
     3661 aaatgatgtg atatagggat ccgtcttcga tcggatgcat tcttggacat attttcccac
     3721 ggttgaatcg gtcggttcaa aggtccagat ggtgtcgtac cctgctgctc gtaatttctc
     3781 cgagactaac tttgatatgg tggattttcc tgagccgtcg atgccttcaa atgtcacgaa
     3841 ctgtttcatg gtttctgttt ctcctgtttc catttgttgc tatagtaact gagtgggtga
     3901 tgtatttttt tgcagagttc gtccatttta tcgaccgggc atatcccgta ggtacgcatc
     3961 ttctcgcatc cggggcattt atactccgtc gatgacgatg cacccgtgat gtgctcgact
     4021 tgatatctgg ttttatcttc ctggtagtcc ggggcggtgc tgaagagctt caagatatca
     4081 ttggtactga gtttcaatga acttaagaat gcgacgaggg aaaaccttcc catatgtggc
     4141 acgttttcac cagattggat cgctgataag atatctttca tacagggtgg gagtttctcg
     4201 atgctgacct tgcctactgg tgttgcctca atttttttct tctggagagc gagcatgttc
     4261 tgaaaccgct ggatatctga agaaaagacg tcgaagacgc ttttgtcaca ttctcggctg
     4321 atgagttctt tgttgattcg ttctcgaagc gcttctaaaa ggattcgtgc aaggtctttt
     4381 tgggttatgg cgaggtatcc tctattgatc ccacggttga ccattttcca ttccttgtac
     4441 cttgttggtg catttcggag ataatctttg aaaaagattt ttatgatgtt tgtctctgga
     4501 atgggttgaa cggttacttc gagttccttt gcgacggtga gaagaaaggg ggttgactcg
     4561 ttgaggagat atttatacgc atgggatgct tctccaagag cgtatcgttt tttgaaaaag
     4621 gaatcaccga tacagactgc gaccatgcgt gctaatgggt aggataagat ttccatgatg
     4681 cagtctgatt cattggcaag acttcgtcta cccacgtcac ggtttttcaa ggcattgtcc
     4741 aatcgttcga cgctgatgat ccgtgctcgc tcgtacgggg gatcatgaag taattcgtta
     4801 atcgagggtc cgttgtcttt gatgaactgt cgtgtatcgt gtaagaatgg atatttggct
     4861 aatgtcacta gctccctcat gacaaacact gatatttctc gtcattaaag aaccttcctc
     4921 ttttgatgta tctctaaatt ttaagtagta gaagaccgat aggggcttga tattccctgg
     4981 gctcgtagct tagaccggtg gagcgactgg ctcataacca gttggtcggc ggttcaaatc
     5041 cgcccgggcc catacccttg attttataaa acaagccaga tacgatgaaa cctcatctct
     5101 atcatctttt tttagggaat agaaaaatta gtgtttcatc gtacattgag aatctttata
     5161 tacagtgatt taatat
//
LOCUS       DATMXJ010000112         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_60002, whole genome shotgun sequence.
ACCESSION   DATMXJ010000112 DATMXJ010000000
VERSION     DATMXJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60002"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VN377_03160"
     CDS             <1..405
                     /locus_tag="VN377_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27418.1"
                     /translation="DVTMVCTNCHRSWNTVAGRISLQPTCSRCGAIKIAVVRRYNKKF
                     LPLISKKHRTAEENKEVRRLHKNASLVLSYGKFAVLALVGRGIGPDTAARILGRYNKL
                     ELAKSEELEVKFLRDVLQAELQYAKTRGFWDS"
     gene            462..1208
                     /locus_tag="VN377_03165"
     CDS             462..1208
                     /locus_tag="VN377_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27419.1"
                     /translation="MKRRIILGSILAVFLIALVPATNAIQIQSMKQESARGPISYEQL
                     KNMDAEELVDFIQILATDYPQLSVEFQRTVEKIENTPTTSIDTNLQRNVLAEKSQGPR
                     QNDSNQTLLEKIFWKILNYRVFRVYLSALLFVFFQSKFTLMRTMTWGIRLLRWVKVGI
                     LLGFIDPTQQPPQTPVIDFQQDLGNDTLTVTYASADVLWSDLSEIGAGSCDPFPTGNV
                     TAGEMITNCTGIIVLQYLPTFEILGVFEFD"
     gene            1327..2913
                     /locus_tag="VN377_03170"
     CDS             1327..2913
                     /locus_tag="VN377_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HWR27420.1"
                     /translation="MPFHQDIIELILSKKIQSKDELHKAKIQLCKKYNLRTIPRDSEI
                     LANLPADQLENGVAHSLLRKKSSRTLSGVAVIAAMTSPEVCPHGRCVPCPGGPERNTP
                     QSYTGYEPAAMRASCNNFDPYLQVRSRVEQLHAIGHVTDKVDLIIMGGTFTARIPEYQ
                     QWFVKRCYDALNTQRSHSLDHAKKKNETAPSRCIGLTVETRPDWFRLQQVDSALDLGA
                     TRVELGVQTIDDRVLSQIKRGHTVSDSIAATRIAKDAGLKICYHMMPGLPGSTEKKDL
                     ETFHTLFTDERFKPDMIKIYPTLVIKGTSLYTLWKEGNYDPLTTEQASHLIATIKKDV
                     PEWIRIQRIQRDIPAQEICTGITKSNLRQYVEAEMQKHHTSCRCIRCREIGHVSSEKT
                     KKVTDRSLRCVHRRYRASDGDEFFISLEDSTQDVIVGYIRLRDVANPHRSELQAEPCM
                     IIRELKVLGREASLGQRTSEAYQHRGFGKKLLTEADSICSEQFGKKRLYVLSGVGVKP
                     YYRRLGFSDDGVYLSKVVSV"
     gene            3218..3838
                     /locus_tag="VN377_03175"
     CDS             3218..3838
                     /locus_tag="VN377_03175"
                     /inference="COORDINATES: protein motif:HMM:NF008993.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HWR27421.1"
                     /translation="MNKMNYNELLKRVHSATVKERVNDDRFKVPKVDVFYEGNTTVLK
                     NFDKIIDVLNRDADHFLKFLLGSVGTAGDISSGRVIFQGKIPIRTIQDRLDEYVETYV
                     ICSECNSPDTHLLKKDRTLLIRCDACGAFRSIGSMKKRKTVVPSEVFKEGEIYELTIK
                     DIGKRGDGVAFFDKYVVYIPGAVKGATVKVKIEKISGTVAFGRVMQ"
     gene            4165..4392
                     /locus_tag="VN377_03180"
     CDS             4165..4392
                     /locus_tag="VN377_03180"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWR27422.1"
                     /translation="MENDRITIRLPQIDLRQIDLLIRLGEFSTRSEVLRHAVKEYIDN
                     HTTRILAKADKFKKIQELEAAAAAIEPYMKK"
     gene            4504..>5428
                     /gene="ftsZ"
                     /locus_tag="VN377_03185"
     CDS             4504..>5428
                     /gene="ftsZ"
                     /locus_tag="VN377_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWR27423.1"
                     /translation="MQSFVETAIENKVTEESKQKVDDGLFGNARIVIVGCGGAGGNTI
                     TRLHKLGVKGAETIAINTDKIALDLVDADKKLLIGKSLTKGLGAGGFPEQGERAARES
                     SRELEEMLQGADLVFVCAGMGGGTGTGSAPVVAEIAKKLGAIVTCMVSTPFNVERARL
                     VKADEGLDKLRVTADSTVVLDNNRLLEFVPNLPINQAFSVMDQLVAETVKGISETISV
                     PSLINLDFADMKAVMDSGGLSVMLWGEADEDAGVDTIVKEALNHPLLNVDYHGAHSAL
                     VHITGGPNMSLKYVQDVAKALTEDMDCYSNVI"
BASE COUNT         1606 a         1101 c         1262 g         1459 t
ORIGIN      
        1 gatgtcacca tggtgtgtac caattgtcat cgttcgtgga acacggttgc aggtcggatt
       61 tctttacaac caacgtgttc ccggtgtggc gcgattaaaa tcgcagtagt tcgacggtat
      121 aataagaagt ttctgccatt aatttcaaag aaacatcgga ctgcggaaga aaacaaagaa
      181 gtcagacgtt tacataaaaa cgcgagtctt gtgttaagtt atgggaaatt tgctgttctt
      241 gcgttggttg ggcgtgggat cggccctgat acagcggctc gtatcctggg tcggtacaac
      301 aagcttgaac tggcaaaatc tgaggagctg gaagtaaaat ttttacggga tgtcctgcaa
      361 gcagagctgc agtacgcgaa aacccgtgga ttttgggatt cataaggaac agtagtaata
      421 tttatatgat gtatgtatat attataattc ggtgataata gatgaagaga cggataattt
      481 tagggagtat tttagctgtt tttttaatcg ctcttgttcc agcgacaaac gcaattcaga
      541 ttcaatcaat gaaacaagaa tcagcaagag gaccaatctc atatgagcag ctgaaaaata
      601 tggatgccga agaactggtt gattttatcc agattcttgc taccgattat ccacaacttt
      661 ctgtggaatt tcaaaggact gttgagaaaa tcgaaaatac accaactaca tcaatagaca
      721 ccaaccttca aagaaatgta ttagctgaaa agagtcaagg gccacgacaa aacgatagca
      781 atcaaacatt gctcgaaaaa atcttttgga agatattgaa ttatcgtgtc ttccgagtct
      841 acctcagtgc tctcttgttc gtatttttcc agtcaaaatt cactctgatg cgtacgatga
      901 cctggggtat tcgactgctt cgatgggtga aagtagggat tctccttggg tttattgatc
      961 cgacccagca accccctcag acaccggtta ttgattttca acaggaccta gggaatgata
     1021 ccttgactgt cacctacgct tctgctgatg tcctctggag tgatctctcc gagataggag
     1081 ctggcagttg cgatccattc cccacgggga atgtgaccgc tggtgaaatg ataacgaact
     1141 gtaccggaat tattgtactc caataccttc ctacctttga gattctcggt gtttttgaat
     1201 ttgattaaca gtcacaaata aaagcgaaaa cagccgatca tcttaaacac agacgttcaa
     1261 cctatattct ttttttttta aattaggacg aaggtttttt agctgaaggt aatatacggc
     1321 caacacatgc cgtttcacca ggatatcatc gaactgattc tttcaaaaaa aattcagtca
     1381 aaagacgaac tgcataaggc caaaattcaa ttgtgtaaaa aatacaatct tcggaccata
     1441 ccgcgcgaca gtgaaattct cgcaaatctt ccagccgatc aactagaaaa cggggtggca
     1501 cattcgttac ttcggaagaa atcgagccgt actttatccg gtgttgctgt gattgccgcc
     1561 atgacatccc ctgaagtgtg tcctcatggc cgatgtgttc cttgccctgg tggtccagag
     1621 cgtaacacgc cgcaaagtta taccgggtac gaaccggcgg ccatgcgtgc cagctgcaat
     1681 aatttcgatc cctatctgca ggtacgtagt cgcgtcgaac aactccatgc aatcggacat
     1741 gtgaccgata aagttgacct gattatcatg ggcggcacgt ttactgctcg catcccagag
     1801 tatcaacaat ggtttgtgaa acgatgctac gacgccctga atactcaacg atcgcattca
     1861 ttagaccatg cgaaaaagaa gaacgagacg gcaccatcac gctgcatagg gttgacggtt
     1921 gagacacggc ctgactggtt tcgattacaa caggtcgatt ccgctttgga tctcggagcc
     1981 acacgagtcg agttaggggt acaaaccatc gatgataggg tcttatccca gatcaagcgg
     2041 ggacatacgg tttctgattc cattgcggca acacgcattg caaaagatgc gggtctgaag
     2101 atctgttatc atatgatgcc tggactcccc ggtagtactg aaaaaaaaga tctggagacc
     2161 tttcacacac tttttaccga tgaacggttc aaaccagaca tgattaagat ttatccgaca
     2221 cttgtcatca aaggaacatc actgtatact ctttggaaag aaggtaacta tgacccgctc
     2281 acgacagagc aagcctcaca tctcatcgca acgataaaaa aggatgttcc tgaatggatc
     2341 cggatacagc ggatacaacg agacatccct gctcaggaga tttgcacggg aattacgaaa
     2401 agcaatctcc ggcaatatgt cgaggcagaa atgcagaagc atcacacatc ctgtcgatgt
     2461 attcggtgtc gagaaattgg acatgtctcc agcgagaaaa caaagaaggt gaccgatcgt
     2521 tcccttcgat gcgtgcatcg tcgataccgg gcaagcgacg gagatgaatt ttttatttca
     2581 ctggaggata gcacgcagga tgttattgta ggctacatac gtttacggga tgtcgccaat
     2641 ccgcatcgtt ccgagcttca agcagaaccg tgtatgatca tccgggagct gaaggtcctt
     2701 gggcgtgaag catcgcttgg gcagaggacc tctgaggcgt atcaacatcg tggttttgga
     2761 aaaaaacttc tcactgaagc cgatagtatt tgttctgaac agtttggaaa aaaacgatta
     2821 tatgtgctca gtggagtcgg tgttaaacct tactatcgaa gattaggttt ttccgatgat
     2881 ggggtgtacc tctcgaaggt agtatctgta taatttgtac tgtttttttt acaaaattat
     2941 ataaacctta gttgatttct ccgcttacaa ccagattggt tgggggagag ttattcatgt
     3001 ccgaattcat atggtattgg acaaaagaaa ataaaaagat atttacaacg caagttgacc
     3061 tcgcggaaca agctatgaaa gatggttttt ttgtaatggg aactcttgtc cgacctattc
     3121 gttcagaata ttgacattgt ttttttctct attttcttca tttctttttc ttttacggaa
     3181 gatttttatt tgtccttgta tttcgccatc ttacaatatg aataagatga actataatga
     3241 attactgaaa cgagtgcata gcgcaacagt caaagagcgt gttaatgacg atcgttttaa
     3301 ggtgccgaaa gtcgatgtgt tctatgaagg gaacactact gttttaaaga actttgacaa
     3361 aatcattgac gtgttgaatc gtgatgcaga tcatttcttg aaattcttgc ttgggagtgt
     3421 tggtaccgct ggtgacatct ctagtggtcg agtcattttc caggggaaaa tcccgattag
     3481 gacgattcag gatcgacttg atgaatatgt cgaaacctat gtgatttgtt ctgaatgcaa
     3541 tagccctgat acccatttat taaaaaaaga tcggacgctg ttaattcgtt gcgatgcatg
     3601 tggagcgttc cgttcgattg gctctatgaa aaagagaaaa accgtcgtgc caagcgaggt
     3661 attcaaggaa ggagagattt acgagctgac gataaaggat attggcaagc ggggtgatgg
     3721 tgtggcgttt tttgataaat acgtcgtcta catccctggc gcggtcaagg gagctaccgt
     3781 gaaggtaaag atagaaaaaa tttctggtac ggttgctttc ggtcgtgtca tgcaatgaga
     3841 accgctgctt tttataaaaa attaaaaatt ataaatttat cacctgtgtc agtatgtgca
     3901 ttagtacctg catacctcta tgcatcggat caattttttt gcggatttcc agatataaaa
     3961 cagacatttt ttcataaata aaatacattg ctaggtctgt cgtattgggc ctgaaatata
     4021 gaaacttatt taaacacaag tacgttttcg ttctatgttt gtctgacaaa tgatgtcgtt
     4081 cagcatttgt ttgacgctcg caacacgcac gtctgacaca acaatgacat caaaatgaca
     4141 aacacgaaaa atgtgaggag aactatggaa aacgaccgca tcacaatccg attacctcaa
     4201 atagatctca gacaaatcga ccttttaatt cgactgggag aattttcaac acgatctgag
     4261 gtattacgac atgcggtaaa ggaatatata gataatcaca caacccgtat tcttgcaaaa
     4321 gccgacaaat tcaaaaaaat tcaagaattg gaagctgcgg ccgctgctat agaaccttac
     4381 atgaaaaaat aaaaatcaag gtaaaaacag gggatgggat gcaccccttt agtgaaggcc
     4441 gtctttttca gacggccttc ccaactacca gaataggagg acgaagaaat ggccgaaaat
     4501 aaaatgcagt cctttgttga gactgctata gaaaataaag taactgagga aagcaagcag
     4561 aaggttgatg acggactctt tggtaatgct cggattgtaa ttgttggatg cggtggagct
     4621 ggaggaaaca ccataaccag acttcacaaa ttaggtgtaa aaggagcaga aaccattgct
     4681 atcaacaccg ataaaatagc tcttgatctt gtcgatgccg ataaaaaact actcatcggg
     4741 aagagcctga ccaaaggact tggtgcagga ggattcccag agcaaggaga acgcgctgca
     4801 cgagaatcaa gcagggagct tgaagaaatg ttgcagggtg ccgaccttgt ctttgtctgc
     4861 gcgggaatgg gtggtggtac cgggaccggt tcagcccctg tcgtcgcaga gattgcaaag
     4921 aaactcgggg caatcgtcac ctgcatggta tccactccgt tcaatgtgga gcgggcacga
     4981 ttagtgaagg cggatgaagg cctcgacaaa ctgcgagtca ccgcggacag cacggtggtt
     5041 cttgacaata accgattgct tgaatttgta ccaaacctgc cgatcaacca agcgttttca
     5101 gtcatggatc aattggtagc agaaaccgtg aagggaattt ctgaaaccat ttcagtgcct
     5161 tcgttgataa accttgactt tgctgatatg aaagcggtca tggattccgg cggtctgtct
     5221 gtcatgctct ggggcgaagc tgatgaggat gctggagtag atacgattgt aaaagaggca
     5281 ttaaatcatc cgctgctcaa cgttgattac catggcgccc atagtgcact tgttcatatc
     5341 actggcggtc cgaacatgag cctgaaatat gttcaagacg tcgcaaaagc actcaccgag
     5401 gatatggatt gttactccaa tgttatcc
//
LOCUS       DATMXJ010000113         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_65196, whole genome shotgun sequence.
ACCESSION   DATMXJ010000113 DATMXJ010000000
VERSION     DATMXJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65196"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1999
                     /locus_tag="VN377_03190"
     CDS             65..1999
                     /locus_tag="VN377_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27424.1"
                     /translation="MGKKIVGILVIGLVFALVFPVGSISASSTDSSIKKITTETHEST
                     SVLFSSQPMLSEKDGFVEIQIEGATTQLLEPNKPVLPIYVRTYQIPFGATDVQVTCAV
                     KNLGSLHLNQDVIPARIASSSSLSEQTAYVKDPVVYGSSEFYPSSWYRYDLGAGRNEN
                     DQQVTFVKVICYPVRYSPMTNEISITGGFDVTISYNEPQTPLTSSTEEYDMIIIAPEK
                     FSSSLQSLIDFKNSKGVITKFKSVESILDEYDGYDAPEQIKYFIKNEYDTSNITYVLL
                     VGGLKSHIFAKDKDTSSAGWTGWWVPVRYVNMPHEDDEGCLSDLYYGCLYNGTGGFDS
                     WDSNEDGVYAAWNKPGSLKDTFDLYPEVYVSRLPCVTDREVKAVVRKILTYEGTSPDT
                     KPWYNNFVGVGGKTGEYYLGKPDGEYLCDLAYNYTKIAIPELRLTTCYSTNRDTGGRT
                     PISEDILKSINEGAGFVDFEGHGNPFSWNTIWFDGEYPEDWTGGFSIYSYPFVRNRDK
                     LPVVIVGGCHNAMYNVSLIPAMLDTNRTKTRYFCYGMPVPVCFCAGLVLKNRGGAIAS
                     AGSTGYGIGYVGYAVSLSGELESNFFYEIGHGSTHVAQAHNHAIQKFLAEEEIQQTEA
                     FVITNWALLGDPSLQFGGYS"
BASE COUNT          625 a          459 c          497 g          611 t
ORIGIN      
        1 gtacaataaa gaggaaacaa gtcttttgta gtgtcttata tataataatt attgaggcaa
       61 tgctatggga aaaaagatag ttggtatatt ggttatcggg ctggtgttcg cattagtttt
      121 tccagtagga agcatttctg cgtcatctac agactcttca ataaaaaaaa taacaactga
      181 aacacatgaa tcgacatcag ttctcttttc gtcccaacct atgctatcag agaaagacgg
      241 attcgttgaa attcaaattg agggtgcgac aactcaattg ttagaaccaa ataaaccagt
      301 gttgcctatc tatgttagga cctatcagat tccttttgga gccaccgatg ttcaggtgac
      361 gtgcgcggta aagaatctag gttcgttgca tctcaaccaa gacgtcattc ctgctcgtat
      421 cgcttcatca tcatccttga gtgaacagac tgcatatgtg aaggatcctg tggtttatgg
      481 gagttctgag ttttatccca gctcctggta tcgatatgat cttggcgctg gacgcaatga
      541 gaatgaccag caggtgacct ttgtcaaggt gatctgttac ccggtccgat actcacccat
      601 gaccaatgag atctcaatta caggcggttt cgatgtcacg atttcatata acgaaccaca
      661 aactcctcta acgtcttcaa ccgaggagta tgatatgatc attatcgctc ctgagaagtt
      721 cagctcaagt ctgcaatcac tcattgattt taaaaacagc aagggggtca tcacgaagtt
      781 caaatctgtc gaatccatcc ttgacgaata tgatggatat gatgctcccg aacagatcaa
      841 atacttcatt aaaaacgagt atgatacttc caatatcacc tatgtgttgt tagtcggtgg
      901 attgaagtct catatttttg caaaggataa ggacaccagc tccgcaggct ggacaggttg
      961 gtgggttcca gtccggtacg tcaacatgcc ccatgaggac gatgaaggat gcctctccga
     1021 tctctattac ggttgtttgt ataacggaac cggtggtttt gacagttggg attcaaacga
     1081 agatggtgtc tatgccgcct ggaacaaacc tggttctttg aaagatacgt tcgacctcta
     1141 tccagaggtc tatgtttctc gattaccctg tgtgactgac cgcgaagtca aagccgtcgt
     1201 gaggaagatt ctgacctatg aaggcacaag ccctgatacc aaaccatggt acaacaattt
     1261 tgttggagtc ggcgggaaaa caggagagta ttatcttggg aaacctgatg gggagtatct
     1321 ctgcgacctg gcatataatt atacaaagat cgcgatccct gagttacgac tcaccacatg
     1381 ttacagcaca aaccgtgaca cgggcggacg aacccctata tcggaggata tcctcaaatc
     1441 aatcaatgaa ggcgcagggt ttgtcgattt cgaaggacat gggaacccat tctcatggaa
     1501 tacgatatgg tttgatgggg aataccctga ggattggaca ggcggtttca gcatatacag
     1561 ctatccattt gttcgcaatc gtgacaagct ccccgttgtt attgtgggcg ggtgccacaa
     1621 cgcgatgtac aatgtatcat taatccctgc catgctcgat acaaaccgca caaaaaccag
     1681 gtatttctgc tatgggatgc cagtacctgt ctgtttctgt gctggtcttg tcttgaaaaa
     1741 ccgaggtggt gcgatcgctt cagcaggtag taccggatat ggaattggat atgtcgggta
     1801 tgcagtgagc ctcagtggag agcttgaatc gaacttcttc tatgaaatcg gtcatggttc
     1861 aacacatgtt gcgcaggctc ataaccacgc gattcagaaa ttccttgctg aagaagaaat
     1921 tcagcaaaca gaagcatttg taataaccaa ttgggctctc ctcggcgacc caagtctaca
     1981 gttcggtgga tattcctagt aagaacgggt atttcatcct ctttcttttt tttttaaaat
     2041 tatatggagt cagagggaga aaactgcatt catatctctt cccattataa caaataatat
     2101 cgtacaaaaa atgatgacaa tatcatcata gaaagttatt ttaatctgca atcgttgtgt
     2161 aacctggatg aaggggagac gaaattctat ca
//
LOCUS       DATMXJ010000114         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_75566, whole genome shotgun sequence.
ACCESSION   DATMXJ010000114 DATMXJ010000000
VERSION     DATMXJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75566"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VN377_03195"
     CDS             complement(<1..228)
                     /locus_tag="VN377_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557185.1"
                     /note="Modulates Rho-dependent transcription termination;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HWR27425.1"
                     /translation="MENTSSEHTDTEISSIFTIKTQVGKEQNTADLINSRAKKGKFNI
                     PSILVTPDLRGYIFVEAFDEELIKNMIKTISY"
     gene            complement(246..500)
                     /locus_tag="VN377_03200"
     CDS             complement(246..500)
                     /locus_tag="VN377_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HWR27426.1"
                     /translation="MNEERKGFLEKAWVLQHKIDERQQRIGKGKYGRVLKMARKPTSE
                     EYEKTAKITGLGILLIGALGFVIYIIRELVAPWILKALGY"
     gene            complement(564..1631)
                     /gene="ftsZ"
                     /locus_tag="VN377_03205"
     CDS             complement(564..1631)
                     /gene="ftsZ"
                     /locus_tag="VN377_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWR27427.1"
                     /translation="MRATKRDPTLKVAGVKDEDLENVLKSLTTTIKVVGCGGAGTNTI
                     TRCFGEGINGAELISINTDAQHLLLAQAPYKILIGRHLTRGLGAGSLPQVGEEAAKES
                     EQDIRDAISPSDMVFVTCGLGGGTGTGCAPVVAQIAREAGALTIGVVTLPFGVEGQVR
                     MDNAEAGLKRLREVCDTVIVIPNDKLLDIVPNLALNAAFKVADEVLMRSIKGITEMIT
                     MPGLVNLDFADLKTVMKRGGVALIGLGEAEGDNKAVDAITEALNSPLLEVDITAATGA
                     LVNVTGGNDMTLREAEKVVEEIYSKIDKNARIIWGTTIDPTMKKSIRAMLVITGVKSK
                     QIMGPTQRLREEQEFGIDFVS"
     gene            complement(1801..2622)
                     /gene="nadC"
                     /locus_tag="VN377_03210"
     CDS             complement(1801..2622)
                     /gene="nadC"
                     /locus_tag="VN377_03210"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWR27428.1"
                     /translation="MDDIDRFLREDIGKKGDITSDALFTKETARAVIIAKEECVVAGL
                     LEAQQVFKRVGASARLLFKDGSVVRKGNSVMEVRGLTRSILKGERLALNIIGRMSGIA
                     TETKKIVQRCKKINSKVTIAATRKTTPGFRMFEKKAIIIGGGEPHRYGLYDAVLIKDN
                     HLKMISPIEEAVVKIKRKLPGTPIEIEVENETDALTAARLNVEIIMLDNFKPKTGKNV
                     ARRIRRINPGILIEVSGGINSDNIESYASFADRISLGYLTHSVKNKDFSLEIFKN"
     gene            2676..>3109
                     /locus_tag="VN377_03215"
     CDS             2676..>3109
                     /locus_tag="VN377_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWR27429.1"
                     /translation="MRLGIIGCGAIGTDVGIAADAMEDIEKIYLHDINPVAAKKLCSM
                     MKKASIQPVEDFLKDVDVVFEAASQQAVTQYAAMILEAGKDVIIMSIGSLFNDTLRKK
                     LENTAKKQHRKIYLPSGAVCGIDGVLAASIEKLDSVTLVTTKP"
BASE COUNT          763 a          764 c          627 g          955 t
ORIGIN      
        1 atacgatata gtcttaatca tgttctttat gagttcttcg tcaaaggctt caacgaaaat
       61 atacccccgt aagtctgggg taacaagaat cgagggaatg ttaaatttac ctttttttgc
      121 tctgctgtta attaaatcag cggtattctg ctctttcccc acctgtgttt taatagtaaa
      181 tatgctagaa atctcggtgt cagtgtgctc agaactagta ttttccatag atgcgaccaa
      241 ccctcttaat agccaagtgc cttaagtatc cagggtgcaa caagttcccg tatgatgtag
      301 ataacaaaac ccaatgcccc gatgagtaaa atccctaacc ctgtgatttt cgcggttttt
      361 tcatactctt cgctggtggg tttccgagcc attttcaaga ctcggccgta cttacccttc
      421 ccaatgcgtt gctgccgctc atcaatcttg tgttgtaaaa cccatgcttt ttccaggaaa
      481 cctttgcgtt cttcgttcac tcttgacacc tgtgatacgt aagggtcaaa tagtaaggtg
      541 tatataaaga atttggcgtt tttttaagag acaaaatcaa taccaaactc ctgttcttcc
      601 cgtaaccgtt gtgtcggacc catgatttgt tttgacttca cacctgttat cactaacatc
      661 gcccgtattg atttcttcat ggtcggatcg atcgtcgtac cccatataat ccgagcgttc
      721 ttgtcgatct ttgaatagat ttcttcgacg actttttctg cttcacgcag cgtcatgtcg
      781 ttcccaccgg taacgttgac caacgcaccg gttgctgcag tgatatcaac ctccaataag
      841 ggtgagttta gcgcttctgt gatcgcatca acagccttgt tatctccttc agcttccccc
      901 aggccaatta atgcgacacc accacgtttc atcaccgttt tcaggtcagc gaaatcaagg
      961 ttcaccagac caggcattgt gatcatctca gtgattcctt taatggaccg catgagtact
     1021 tcgtcagcga ctttaaatgc agcgttcaac gccaggtttg ggacgatatc gagtaattta
     1081 tcattcggta tgacgataac ggtgtcgcat acctcgcgga gtcgtttcaa gcctgcttcg
     1141 gcgttatcca tcctgacttg cccttcgacg ccgaatggaa gggtgacgac accgatggtc
     1201 agtgcaccgg cctctctggc gatctgtgcg acgacagggg cacatccggt ccctgttcct
     1261 ccaccaaggc cacaagtaac aaaaaccata tctgatggac tgatggcatc tcggatgtct
     1321 tgttcgcttt ctttcgcggc ttcctcccct acttgcggaa gagagcctgc tccgagacca
     1381 cgggtgagat gtctcccgat aaggatctta tagggagcct gagctaacag gagatgttgt
     1441 gcatcggtat taattgagat gagttcagca ccgttgattc cttctccaaa acagcgtgtg
     1501 atggtgttcg tccctgctcc accgcagcca acgaccttaa tcgtcgtcgt cagacttttc
     1561 agcacatttt ctaaatcctc atccttcaca cctgcgacct ttaaggtcgg atcgcgtttc
     1621 gtcgctcgca tcccaacgct cttcttttcc tcctgattta tctgttcttc cacgttctct
     1681 tttatattct cagctgcaac tacatcttca ataatcttct tcatttatca actcaccatc
     1741 ctcacaataa aaccccatct ttgtagagaa ttattttttt cccaatattg taaactaatt
     1801 tcaattctta aatatttcca aggaaaaatc tttatttttc accgaatggg tcaagtaccc
     1861 aagggatatt cgatcggcaa atgaggcgta cgactcgatg ttatctgaat taatgccacc
     1921 agacacttca ataagtatgc ccgggttgat ccgccggatc cttcgagcaa cattttttcc
     1981 ggtttttggt ttaaaattgt caagcatgat tatctcgacg ttcaaccttg ccgctgtgag
     2041 cgcatctgtt tcattttcca cctcgatttc aatgggagtg cccgggagtt ttctttttat
     2101 ttttacaaca gcctcctcaa tcggactaat catcttgaga tgattatctt tgataagcac
     2161 cgcgtcataa agcccataac gatgcggttc tcccccaccg atgatgattg cttttttttc
     2221 aaacatgcgg aagccagggg ttgtctttcg tgtcgcagcg atcgtcacct ttgagtttat
     2281 ctttttacac cgctggacaa ttttttttgt ctcggtggca atcccactca tccttccgat
     2341 gatgttcaac gcaagtcgtt cgccctttaa aatagaacga gtcaatcctc gtacctccat
     2401 gaccgaattt cctttccgaa cgacagaacc atctttgaag agcaatcggg ctgatgctcc
     2461 tactctcttg aacacctgtt gtgcctccag caagcctgct accacacatt cctcttttgc
     2521 gatgatgact gctcgtgctg tttctttggt aaacaacgca tctgaggtga tatctccttt
     2581 ttttcctata tcttcacgta ggaaacggtc aatgtcatcc ataacaatat tttttatagg
     2641 taattcctgt ttaaaggttt tgggagaatc catacatgag gcttggcata attggatgtg
     2701 gcgctattgg gaccgatgtc ggaatagcag cagatgcgat ggaagatatc gagaaaatct
     2761 atctgcatga tatcaaccct gttgcagcaa agaaactctg ttcaatgatg aaaaaagcat
     2821 cgattcaacc agtcgaggat tttttaaagg atgtcgatgt tgtctttgaa gcagcttccc
     2881 agcaagctgt cacccaatat gcagcgatga ttctcgaggc aggtaaagat gttattatca
     2941 tgagtatcgg tagtctcttt aatgatacat tacgtaaaaa gctagagaac accgcgaaaa
     3001 aacaacatcg gaaaatatat cttccctctg gtgcggtctg tgggatcgat ggtgtgttgg
     3061 ctgcaagtat cgagaagctc gatagtgtga cgttggtcac gactaaacc
//
LOCUS       DATMXJ010000115         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12891, whole genome shotgun sequence.
ACCESSION   DATMXJ010000115 DATMXJ010000000
VERSION     DATMXJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12891"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VN377_03220"
     CDS             complement(<1..535)
                     /locus_tag="VN377_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27430.1"
                     /translation="MALSIILVFAGLTISPALHASSLENIPIYDQVDLIINDDGCGDP
                     TDNEVDVPSYTPDSGITPTITINFTILGTNSSEVTAYYGDDPWEDWSNIIVTGDILFP
                     VNETTLYHIGSKGDWICKVTPTKPGGIIDLFILWDGEGADEEIIQIVNGLVVHPAVDS
                     FIWGADCNLTVTIIDMDG"
     gene            complement(795..2102)
                     /locus_tag="VN377_03225"
     CDS             complement(795..2102)
                     /locus_tag="VN377_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR27431.1"
                     /translation="MFKKRFGSFLDLNVKRIGQYSKDDLLDLILHLCNTVDFAENGSK
                     TFQLQRTNVPNGDTLLHHLKGYQDIDQIKHMFIAMFEMIWETAKKANQLQRIVDCSID
                     YTSQHFYGDKGAPMVTEMKPDHGTAYDYKFATITISEHNQRFTLLAVPVGPFDTKEDI
                     LNTLLDYALQRVKIRCLYVDRGFFDSNCIELFKRYHLKFLMPCTVNERIKRILEVVPS
                     PTVIKDYEMKKTTFNVIVISDEFHIKRAFATNIDFNENDVGLAERLFNLYSKRWTIET
                     SYRVKKHSFRPKTTSKNYFIRLFYFLFSALMYNLWLLADILICLAMLDFYNGKHIITS
                     NTIEQNENGLNLAGTPFTTVSQNNFLNNENDANFTALITKIIGVFTCIGTRFQGNYWG
                     EARLLPKIIPGTIDFTRDGQNFTRFNLLKIDWHPAQEPYDIGV"
     gene            complement(2598..2732)
                     /locus_tag="VN377_03230"
     CDS             complement(2598..2732)
                     /locus_tag="VN377_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27432.1"
                     /translation="MKPLEELANSGRMNIHHFGYLELGFPPQDLFSHLLNVILGRFSS
                     "
     gene            complement(2758..>3512)
                     /locus_tag="VN377_03235"
     CDS             complement(2758..>3512)
                     /locus_tag="VN377_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27433.1"
                     /translation="LFTTQIVVLDKTAFCTVTNLNLTALFTLPGLAVPSSFESKYCFN
                     NFYFDNYFSYNTFAMNLMRCRNCLIKNNTYINNDLALSVDNSQRNRVENNYFYDNYDG
                     IVLGGMGIFIPGLCSGNIIEKNLILHCRNGIRMCIIPFVPLGMIIKNTITANEIRNCT
                     VGFLGAGGSYLNVFYHNNFIDNEVNAGGRGINIWYNSKLGGGNYWDDYQGVDADGDGI
                     GDTPYTTINYRPFGIIWDKYPLMEPIRFQEFK"
BASE COUNT          960 a          693 c          771 g         1088 t
ORIGIN      
        1 ccccatccat atctattatt gtgactgtca ggttacaatc cgcaccccag ataaaagaat
       61 ccaccgcagg atgaacaacc aatccattga cgatttggat gatctcctcg tcagcacctt
      121 ccccatccca aaggataaag agatcgatga tcccccctgg tttcgtcggt gtgactttgc
      181 atatccaatc ccctttcgat ccgatatggt agagtgtcgt ctcattcaca ggaaagagga
      241 tatctcctgt tacaatgatg ttactccagt cctcccacgg gtcatcacca taatacgccg
      301 tgacctctga actatttgtt cctaggattg tgaaattgat ggtgatcgtt ggcgtgatcc
      361 ctgagtcagg agtgtaggac gggacgtcca cttcgttatc ggttggatct ccgcagccgt
      421 catcattgat aatgaggtca acctggtcat aaatcggaat gttctctaag gaggacgcgt
      481 ggagtgcagg gctgattgtt aacccagcga atacaagaat gatgctcaac gccatggctt
      541 tgcttaattg aggatggttt tccaaatcat tccgcccctt tctctattta taagtaatat
      601 ctcctataat aacattattc cttgaaaaaa ttgtcattgc tcatgatact ttgacagtca
      661 taacagatgt cagtctgatg atcatcaggg agctctgagc cgcagatggc gcagtatcgt
      721 gtcatggatt tttgccttcc aacttcctag tctgaaaaag ctattcaatg agattagaat
      781 ctttcatctg gtttttatac tccaatatca tagggttctt gagcaggatg ccagtctatt
      841 ttaagtagat tgaaacgggt gaagttctgt ccatctcttg taaaatcaat agttccgggt
      901 ataatttttg gtaacaatcg tgcttcgccc caataatttc cctgaaacct tgtgccaatg
      961 caggtgaaaa caccgattat ttttgttatt aaggcggtga aatttgcatc attctcattg
     1021 tttaggaaat tgttttgact gactgtcgta aaaggggttc cagcgaggtt gaggccgttt
     1081 tcattttgct ctatagtatt tgatgtgatg atgtgcttac cgttgtagaa gtctagcatg
     1141 gcgaggcaga tgaggatgtc tgcaagcagc cagagattat acatcaaggc tgagaagaga
     1201 aagtagaaga gtcggatgaa ataattcttg gatgtcgtct tcggtctgaa tgagtgtttc
     1261 ttgactcggt agcttgtttc aatggtccat ctttttgagt agagattgaa cagtcgttct
     1321 gcaagcccta catcgttttc attgaagtcg atgtttgtgg cgaaggcgcg tttgatgtgg
     1381 aactcgtcgc tgatcacgat cacgttaaag gttgttttct tcatttcgta gtccttgata
     1441 acagtaggag aaggaacgac ttcaaggatt cttttgattc gttcgtttac ggtgcaaggc
     1501 atgagaaact tgaggtgata gcgtttgaag agttcgatgc agttggagtc gaagaagcca
     1561 cgatcaacgt ataagcatcg aatcttcacg cgctgaaggg cgtagtcaag aagggtgttg
     1621 agtatgtctt ccttggtgtc gaatggtcct acaggcacgg caagcaaggt aaatcgctgg
     1681 ttgtgttcgg agatggtgat agtggcgaac ttgtagtcat aggcggttcc atgatcaggt
     1741 ttcatttctg taaccatggg cgcgcctttg tctccgtaga aatgctggct tgtgtagtcg
     1801 atggaacagt caacaatcct ctgtaactgg ttcgctttct tggcggtctc ccaaatcatt
     1861 tcgaacatgg cgatgaacat gtgcttgatc tggtcgatgt cctggtagcc cttcaaatga
     1921 tggaggaggg tgtcgccatt tgggacattc gttctttgga gttgaaaggt cttgctgccg
     1981 ttttccgcga aatccacggt gttgcagaga tgaaggatga gatcaagaag atcatccttg
     2041 gagtattgtc cgattctttt cacgttcaga tccaggaacg agccgaatct cttcttgaac
     2101 aaggagccga tctctttggt cttcgcactt ttgttgcggt agaaactcct gtctgaggat
     2161 gctttctttg gctcttcaat taggatgtgt tcttgatcga gaatgattcc gaatttctct
     2221 gagattgata atgccttaga aacgatgaaa tcaatacttt cttttacttc atcatccagg
     2281 acattgttga agaagttgtt gattgtctcc tatctggtaa ttctatgagt ccgagatgct
     2341 ctcgttcttt cttatgggtt cgtaagtagt gttctagctg tgtctggaat cgaagcttac
     2401 atttaatctt caagtggtag aggaagttat tgcgcatacc taactccctc taaaaaccaa
     2461 ctaagagcca tcagaggttc aattaggggg agtgggtccg gtcttggagt gtttaagaag
     2521 atctcgaaga agtcttgacg aactgctagt tttagaaacg tcctgtcacc accatatcaa
     2581 atccctatga acatccatca ggaagaaaag cgtccaagga tgacattcag aagatgagag
     2641 aaaaggtcct gagggggaaa tccaagttca aggtatccaa agtgatgaat attcatccga
     2701 ccactatttg ccagttcctc cagaggcttc attttagaga tataataata taaaatttta
     2761 tttaaactcc tgaaatctaa tgggttccat tagcgggtat ttatcccaga taataccaaa
     2821 tggtctgtaa ttgattgttg tatatggtgt atctccaata ccatcaccat ctgcatctac
     2881 cccttggtaa tcatcccaat aatttccccc accaagtttc gaattatacc agatgtttat
     2941 tcctctacca cctgcattta cttcattgtc aataaaatta ttatgataaa atacattaag
     3001 ataagatcca cctgctccga ggaaacctac cgtacagttc ctaatttcat tggctgtgat
     3061 agtgtttttt attatcattc ctaacggtac aaatggaatt atgcacattc ttattccatt
     3121 tctacaatga aggatcagat tcttctcaat gatattccct gaacataagc ctggtatgaa
     3181 aatacccatt cccccaagaa cgatgccatc ataattatca taaaaataat tattttctac
     3241 gcgattcctt tgagaattat cgacagagag tgccagatcg ttattaatat atgtattatt
     3301 tttaataaga caattacgac atctcataag gttcatggca aaggtattgt atgaaaaata
     3361 gttatcgaaa taaaaattat taaaacagta tttcgattca aatgaagatg gaaccgctag
     3421 gcctggtaaa gtaaaaagag cggtcagatt gaggttcgtg acagtacaaa atgctgtctt
     3481 gtcaaggaca actatctgag tcgtaaaaag at
//
LOCUS       DATMXJ010000116         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28020, whole genome shotgun sequence.
ACCESSION   DATMXJ010000116 DATMXJ010000000
VERSION     DATMXJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28020"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1669
                     /locus_tag="VN377_03240"
     CDS             50..1669
                     /locus_tag="VN377_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWR27434.1"
                     /translation="MKHKITTILGIMLVFFASATTVIDHSVLVKGNVSSDNTTLSIEF
                     VSSGFNVDYKFSELFGVSAVIRNTGNVNATNVHWSISSSGGLIVMGKKASGTIPIIFS
                     GDVSIVKIRLMVGFGKTQIIAAANASNANKTEANVRAKVTGLRVTLLPGSEDSMAIRL
                     DRIARGLKAPTVLTSANDGSGRLFIAEQTGTILVIDNGVLQTTPFLDLSSKIVKLIPV
                     YDERGLLGLTFHPDFRNNSRFYVYYTAPTNVSGFDHQNIIAEYQISGENPNVADPNSE
                     RIIMSIDQPQFNHCGGQLAFGSDGYLYIGVGDGGGEGDPNGVIGNGQNINTTLGKILR
                     IDVDHGSPYSVPADNPFVGVDGQDEIFAYGFRNPFRFSFDMTTERLFVGDVGQDLWEE
                     IDIVEKGGNYGWRIMEGNHLFDPALAAYLGINLSMLSPPIYDYSHYVGHTVIGGVVYR
                     GVQYPSLVGKYVYGDWSATYFQPRGKLYYLDEVAPDVWNHREFRFSDDKPLNLRILSI
                     GTDELGEIYVLTQRTIGPLLHTGEIWKIIVE"
     gene            1683..2063
                     /locus_tag="VN377_03245"
     CDS             1683..2063
                     /locus_tag="VN377_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27435.1"
                     /translation="MRSMKVVGIFVIGLLVTISMVPLGNATPPRYLKVQYDVGTSTLK
                     VTLTHATPVRSIHYIYRIIVQKNGVLEQSHFYSKQPGFFINTYEYNLSANPGDVIKVS
                     AFCILFGYNTRSTTVSPIAQSIRI"
BASE COUNT          605 a          416 c          485 g          653 t
ORIGIN      
        1 ctatatggaa taacagattg tacagatttg aaaatttggg gaaaatacta tgaaacataa
       61 aatcacaacc atattaggta tcatgctggt tttttttgca agtgccacaa cagtcataga
      121 tcactcggtt ctcgtcaaag gaaatgtatc atctgataac actaccctga gtattgaatt
      181 tgtcagtagc ggttttaatg tcgattacaa attctctgaa ctttttggtg tgagtgcggt
      241 gattagaaat acaggtaatg tgaatgcgac aaatgttcac tggagcattt cttcatcagg
      301 cggacttatc gttatgggta aaaaggcatc aggtacgatc cctatcatct tttctggtga
      361 tgtttcaatt gtcaagatac gcctcatggt cggatttggt aaaacacaga taatagcagc
      421 agcaaatgct tcgaatgcga acaaaaccga agcaaacgtg cgagcgaagg tgactggact
      481 ccgagtgact cttttacctg gttctgagga ttcaatggcc attcgtcttg atcgcatcgc
      541 tcgcggtttg aaagcaccga ctgttctgac aagtgcgaac gatggttcag gtcgcctttt
      601 tattgctgag caaaccggta caattcttgt tattgacaac ggtgttctcc aaactacacc
      661 gtttcttgat ttgtcaagta agatagtaaa gctcatacca gtgtatgatg aacgcggtct
      721 gctagggttg acgtttcatc ctgatttccg taacaacagt cgcttctatg tgtattacac
      781 cgctcctaca aatgtgtctg gttttgatca tcagaacatt atcgcggaat atcagatttc
      841 cggagagaat cccaatgtgg ctgatcccaa ttctgaacga atcatcatga gtatcgatca
      901 accccagttc aatcattgcg gaggacaact cgcgtttggc tctgacggtt atctttacat
      961 cggggtcggt gatggtggcg gagagggtga tccaaatggt gttattggaa atgggcagaa
     1021 tatcaatacg acacttggga aaatattacg aatcgacgtt gaccacggta gcccctatag
     1081 tgtgccagcc gacaatcctt ttgttggtgt agatggtcag gatgaaatct ttgcgtatgg
     1141 gttccgcaat ccattccgat tttcgttcga tatgacaaca gagcggttat ttgtaggtga
     1201 cgtcggtcag gacctctggg aagaaatcga tatcgtagaa aaaggaggaa attatggatg
     1261 gaggattatg gagggaaatc atctctttga ccctgcgtta gccgcatatt taggaatcaa
     1321 tctcagcatg ctttctcctc cgatctatga ttacagtcat tacgttggtc ataccgttat
     1381 cggcggggtt gtctatcgag gcgttcagta tccatcgctc gtcgggaaat atgtgtatgg
     1441 tgattggagt gcgacgtatt tccaaccacg agggaaatta tattatctgg atgaggtcgc
     1501 acctgatgtt tggaatcata gggagtttcg ttttagtgat gataaaccgc tgaatcttcg
     1561 tatccttagt attggtacgg atgagcttgg agagatatac gtactgaccc aacggaccat
     1621 tggtcctttg cttcatactg gtgaaatttg gaaaataata gtggagtgag gaggtgtcaa
     1681 aaatgagatc tatgaaagtt gttggaatct ttgttatcgg tctgctcgta actatatcca
     1741 tggtgccatt aggaaatgca accccacctc gatatctgaa ggttcaatat gatgtaggta
     1801 cgagtacatt aaaggtgaca ttaactcatg cgacaccagt tcgttctatc cattatatct
     1861 atcgaattat tgttcaaaaa aatggtgttc tcgaacaatc acatttctat agtaagcaac
     1921 ctggtttttt cataaacacc tatgaataca atctgtctgc aaatcccggt gatgtcataa
     1981 aagtttcagc attttgtatt cttttcgggt acaataccag atcaacgacg gtatctccga
     2041 tcgcccaatc catccgaata taaataagaa actgaatgaa aagaacgtcg tcattccttt
     2101 cggcatttct ctagaaaaaa cagagaacat cattcatatc ttctttttct tttattatt
//
LOCUS       DATMXJ010000117         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28023, whole genome shotgun sequence.
ACCESSION   DATMXJ010000117 DATMXJ010000000
VERSION     DATMXJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28023"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1884
                     /locus_tag="VN377_03250"
     CDS             508..1884
                     /locus_tag="VN377_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWR27436.1"
                     /translation="MRTIEGDIPTWYTGDEWTYTVDPLSFSSPNGSFDGSIQNFRQTV
                     VGIADDVYTISITGDISGDVTMTSFSGELSGDVTGTSQVRVSDLAEVTNELHSQGTIT
                     YMWIPFAYELNIYMNSAPVLEVYDFPLQMGEQWQLDSLTTISGSFSIVGVYDQSFEGS
                     QLVDEIVECNQQEQINVPAGTFECFKIGRQTTLSWYSTDVGNMVKTTVDQSGENMTLQ
                     MVAVLQSYSFVNQPITISEEISPSIVASGASVVISGQAISTSSGDPVQNGVVSIQIPS
                     SGGSWSTTTNSGGYYSKIIIAPTMNDDTPSGRETGSGGVVVQCSSSGLTGYRVKTLTT
                     VQDNPPMVPSIHGQSEGKPRVSYSYTVVAVDPENDEVFYYVDWGDGTNSSWVGPYLSN
                     ENLTLNHMFAKKGTYIIKAQARDIYYATSGWGTLQVTMPTEASAFLLKFFHRFPIMYS
                     LLRQFLGQ"
     gene            2398..>3903
                     /locus_tag="VN377_03255"
     CDS             2398..>3903
                     /locus_tag="VN377_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR27437.1"
                     /translation="MKQILVVALCILLLCVGFSGCINHAEEENQKQSELLTIGIAEEP
                     QGFFPWIDSYDTGTLGINFNVFNPLVEFDASFKLFPKLAKSWNNPDNCTWRFFLRDDV
                     TFHNGYNFTAYDVKYTIDLIKNNKSHVLWDLLTEVKEVKVVNNYTVDFETYQPFPILL
                     NKLTNIPMASQRYQEETTNRWPIGTGAYKLQENVPEDHITLVRFEEYTLGHPEIQKVI
                     FKIIPKSEDRKNALITKQIDIMEHVPEMYVEEILNNSGITIKKISNPTVMYLGFDFRE
                     NNSSSYPGMKNPLADVRVRKALYHAIDVNSIIKILGLAAEPASQYLSPLIFGYNPQIK
                     RLPYDLNLSRQLLKEAGYENGFNITLDCPEIYVLQVEICEEIVRQLSQVVNASLNLLP
                     SEEEYYTKLYSGNCSIYFLGWIPATGDGGEIFDYLLRSENHSLGRGSYNFGYYSNRTI
                     DEITSEVSYTMNQKTRMNLMREGFKIAMDDVACIPLYISVCNYAYPEYLEWS"
BASE COUNT         1149 a          801 c          837 g         1116 t
ORIGIN      
        1 ctttcaacgg cctcatagta gccttgaaaa tggacatctg agaacgccct ttcaggcgtt
       61 ccaggataaa atgagggcga aaaacttaag gtgagatgat ggttttaaaa aatcagaagg
      121 aagttaaatc aggaaatcac aatggaagca tcgacgtgaa aatgaatctc gttacgaaaa
      181 gaagaaaaaa tgatatgaaa atatcgtata ttttcttgtg atatcctgtt tcaattgata
      241 caagattttt tccttcatac actctttcaa tcacttttct atagtaatca ataaattttt
      301 atcttattaa aaacatttaa atgtcgctgc tctttatact atgtaacgga tggaaatcat
      361 atgaaacatg gaatgatgaa aaggaatctc aggaaaagtt atgtgaccta tgggatatgt
      421 tttttattcg tttgtataag ttttactcca gcgatgggtg cattttctca gaaccatcat
      481 ccaatgtcgt cttacagcaa aacctcgatg agaacaatcg aaggggacat ccccacatgg
      541 tatacggggg atgaatggac atacactgtt gatcctctct ccttctcgag cccaaatgga
      601 tcatttgatg ggtcaataca aaatttcaga cagacggtgg tggggatcgc tgatgatgtc
      661 tatacgatca gtatcacagg cgacatcagt ggcgatgtga cgatgactag tttttcaggg
      721 gagctttccg gagatgttac tgggacatcg caggttcgtg tttctgacct tgccgaagta
      781 actaatgaac tccattcaca agggacgatt acctatatgt ggataccgtt tgcctatgaa
      841 ttgaatattt atatgaactc tgcccctgtg ttagaagtct atgactttcc cttgcagatg
      901 ggggaacaat ggcagcttga tagtcttacg accatctcag gttcattcag tattgtaggg
      961 gtgtacgacc agtcatttga gggaagccaa ttggttgatg agatagttga atgtaatcaa
     1021 caggagcaaa tcaatgtccc tgctggtaca tttgagtgtt ttaagattgg gcgtcaaact
     1081 actcttagct ggtattcaac tgatgtcggt aatatggtca agaccacggt ggatcaaagc
     1141 ggtgaaaaca tgacgctgca gatggtcgcc gttcttcaat catactcgtt cgtgaatcag
     1201 cctatcacga tttcagagga gatttcccca tcgatcgttg catcaggtgc ctcggtggtt
     1261 atctctggtc aggcgatttc cactagctca ggtgatcctg ttcaaaacgg tgtcgtttct
     1321 atacagattc cttcctcggg tgggagctgg agcacaacaa caaattctgg tgggtactat
     1381 tcgaagataa tcatagctcc gacgatgaac gatgatacgc cgtctgggag agaaaccggc
     1441 tctggtgggg ttgttgtgca atgtagcagc agtggtctta ccgggtatcg tgtgaaaaca
     1501 ttaacgacag tgcaggataa tccgcccatg gttccatcta tccatggtca gtccgaagga
     1561 aaacccaggg tgtcgtattc ctatacggtt gtggctgtag acccagagaa tgacgaggtt
     1621 ttttactatg tcgattgggg tgatggtacc aacagcagct gggtaggacc gtatctgtcg
     1681 aacgagaacc tgacattaaa ccatatgttt gcaaaaaagg gaacgtacat catcaaggca
     1741 caagccagag atatctacta tgcgacaagc gggtggggta cgcttcaagt caccatgcca
     1801 actgaagcat cagcttttct gctgaagttt ttccatcgat ttcccatcat gtatagcctg
     1861 ctgcgacagt tcctcgggca gtaatctttt tcccatcttt cctccttttt tcacacctgc
     1921 tgcacagtat ctcatcgatg aaacccatca cctcgaaata acgaacaaga cgacacttga
     1981 cacatcattc tatactctga cacaggcact caaatcaaat tcagttcatt tctatcgcct
     2041 caccaactca tcagtgatac ctgtggaagg accccccagt ggcaaaaatc cctttcctct
     2101 tacggcttca cttccttgat ccactcaccc gcttccttgc cctcctcatc gttcttttta
     2161 tcctgagcta agaaaaagaa aaaacctagt tgggtaccca tttctcttcg tttactataa
     2221 cgcgacagga gatttggata aaaattattt tttcgtgtaa atattgtgtc tttgtacaaa
     2281 aaatttaagg atacctttta attctctttt actggtaaga gatatgtagg gatactcgat
     2341 tagatctctt agaatagcat ctttagcatt aatgcgtatt tatcagtctg gtagaacatg
     2401 aaacaaatac ttgttgtggc actttgcatc ctcctgctct gcgttggatt ttctggttgt
     2461 atcaatcatg ccgaggaaga aaaccaaaaa caaagcgagt tattgaccat tggtatagca
     2521 gaagagcctc aagggttttt tccatggatt gatagctatg atactggcac cttggggatt
     2581 aattttaatg tcttcaatcc cttggtggaa tttgacgcca gttttaagct gtttccgaaa
     2641 cttgcgaaat cctggaataa cccagataat tgcacatggc ggtttttcct cagagacgat
     2701 gtcacattcc ataacggcta caattttaca gcgtatgatg tcaaatatac catagacctc
     2761 ataaagaata ataaaagtca tgtgctatgg gatctgctca cggaagttaa agaagtcaag
     2821 gttgttaata actatacggt ggatttcgaa acctatcagc cgtttcctat ccttttaaac
     2881 aaactgacaa atatcccgat ggcatcccag agatatcagg aggaaacaac aaatagatgg
     2941 ccaatcggaa ctggtgcgta taaacttcag gagaacgtgc cagaggatca tatcacctta
     3001 gtacgctttg aggaatatac attaggtcat ccggagatcc aaaaagtcat ttttaagata
     3061 atcccaaaaa gcgaagaccg gaaaaacgca ctcattacaa aacagattga tatcatggag
     3121 catgtcccag aaatgtatgt cgaggagata ctgaataatt cagggattac gattaaaaaa
     3181 atctcaaatc caactgttat gtacctcgga tttgacttca gggaaaacaa tagcagtagt
     3241 taccccggga tgaaaaatcc tttagcagac gtccgggtga ggaaagcgct ataccatgca
     3301 attgatgtca acagtatcat aaaaatccta ggcttagccg cagaacccgc ctcgcagtat
     3361 ttatcacctt taatttttgg atataaccct cagataaaac gtttaccgta cgatttgaac
     3421 ctttcccgac agcttctcaa ggaagctggg tatgaaaacg ggttcaatat cacattagac
     3481 tgcccagaaa tttatgtgtt gcaagttgag atttgcgaag agatagtacg acaactttca
     3541 caggtagtta atgccagcct caatctcctt cctagtgagg aggagtatta taccaagcta
     3601 tatagtggga actgctccat ttactttctt ggctggatcc cggcaacggg agatggcggt
     3661 gagattttcg attatctgtt acgatctgag aatcattcac tcgggagagg atcctacaac
     3721 ttcggatatt attcgaatcg aaccattgat gaaatcacat cggaggtcag ctacaccatg
     3781 aaccagaaga cacggatgaa tcttatgcgc gagggattca agattgcaat ggatgatgtt
     3841 gcctgtattc ctctttatat tagtgtctgt aattatgctt atccagaata tcttgaatgg
     3901 agc
//
LOCUS       DATMXJ010000118         8895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7593, whole genome shotgun sequence.
ACCESSION   DATMXJ010000118 DATMXJ010000000
VERSION     DATMXJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7593"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..428)
                     /locus_tag="VN377_03260"
     CDS             complement(69..428)
                     /locus_tag="VN377_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HWR27438.1"
                     /translation="MTPPGNTYSKAHHSHSPQDGEDGYYRLPLPNRKNNELFAIADQL
                     LGGSRIHVVCEDKKSRMARIPGKMKRKARVRAGDLLIIKPWDIQNEKADIVFRYSRTQ
                     ASSLSRRKMLPEEINVF"
     gene            complement(432..986)
                     /locus_tag="VN377_03265"
     CDS             complement(432..986)
                     /locus_tag="VN377_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWR27439.1"
                     /translation="MLTRAEAVPLLKRNLRTENLMKHSFAVEAILEDMARKLGEDPQL
                     WGLTGLLHDLDYDFTKNDPEKHSLVTIKVLNDLLPPEAINAIKAHNYQYTAQIPQTYL
                     DKSLIAADAVSGLIIAAALVMPTKKLADVTPKTLLGKFKDKSFAAGCNRKRIELCEDM
                     ELELQMFLELSLDSLKRISDALGL"
     gene            1098..1403
                     /locus_tag="VN377_03270"
     CDS             1098..1403
                     /locus_tag="VN377_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWR27440.1"
                     /translation="MIVDEEVDFLKQVKTVLEKEDIEVVTARNSREALERLKEENEET
                     FDLILVNTRMPGSTLTNALFSMRPALKKLPGGIENFLQKPFTKEELVEFIKEKIDLH"
     gene            complement(1447..2115)
                     /locus_tag="VN377_03275"
     CDS             complement(1447..2115)
                     /locus_tag="VN377_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27441.1"
                     /translation="MTVAVVMKNKKEKFADTPNFTEKKDYRPSEVKETGLVFVGHEIS
                     EDRTVMNQFLHYDQLYTIRHGWNSKFFIGLLDGKIMGTRCPKCGDSWVPVRTHCWNLD
                     CHLQKTEWVEMPLTAKVHTWTIAGWSGRSSLKRLPIILVYANIGTSKVAMANELHGMN
                     PWDVEFGMPLKIVFKPKQQRIGTVTDFHFEPADFWTPSPMNPEKQRIKDLVTPVYEWV
                     KTLK"
     gene            complement(2119..3369)
                     /locus_tag="VN377_03280"
     CDS             complement(2119..3369)
                     /locus_tag="VN377_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWR27442.1"
                     /translation="MSRKVCIIGGATTPFDYGPTGFESIEGGAAECIKETMQDVPAFS
                     LRDLDLMIYSHFSVHFSHQLAPEWIIHDHLGLVGLPHYRVENGGNTGGSALYAAALAV
                     LSGHHDVVGIVGWETMDSVSQSQGSEFIAMASDIRYELLVGGIYPIYYAAMANKWMKE
                     NHVTEEDVAAIAVKNKNNAMDNPKAQWYRNDHRYITVDDVMNSRLIAYPIKKYDCCLM
                     SRGVAFLLVCSEEYAKKVNPDRYVKVDGMGLSSCTIRAGDRLNNPGWSERYGPGYPGM
                     TEFAACRNSAEHAYKMAGIKDPLKDIDFGEIHDAFSTSEAQIYKALGLANEDTIAKFI
                     RDERTGMNGDIPMNPGGGLLGYGHPVGATGLMSMVESYYQLAGLIPKKHLSKKTEVPD
                     AECGIVNSHAGTGTSISNFILRRH"
     gene            complement(3484..6291)
                     /locus_tag="VN377_03285"
     CDS             complement(3484..6291)
                     /locus_tag="VN377_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR27443.1"
                     /translation="MALGVILFLLIPCIIPSFGQSVGEKTIKSVSYGTLFYVGGSGEG
                     NYSRIQDAIKDSSSGDTVFVYDDSSPYLENVVVDKSITLVGEDKTSTEINGSSLDNSL
                     DTVAVITDHVTIDGFRITDNHGYYYQAAVKIKGNFTSLSNCIINGNEWIGISLVDASF
                     CQIRDCELSENLVAIHLVNSRKNIVQNCVCYDNADAITLFQSSDDNQIIHCTCTRNHF
                     DSILIQQSSGNQITGCVCQDGYDGISLPYAPNTKMRNNTMMGNYANFGIGSSSVSDFY
                     CDIDTSNTINGKPMYYLIEQHDLPFNGTTEIGFLGLVSCDNISVNDCNFSNNFEGILL
                     AGTTDSKIENCSFRNNDGHGIYLISSQDNTVKTCTFQNSFWDGVFLYDSSNNTMENCS
                     YYGSLAGINLGLSTGNILRGQTIDQCNVGISFDASGNNVLKDNEMIQCGLQVTGDDPA
                     GYRNDVDASNTVNGNPVYYYINETNRTIPSDAGQVILTNCNGCIVSQCNLSDASVGLE
                     LAYCSMNTIEDNILTNNSVVAIDLDGSDNNENSISGNIIQGNNYGIDVDSSNMNIFQD
                     NILVDNGMGFSFDSCWKNSIIDNTVQDGSYGMSFDTSSNNILINNTICNTSVFGLYLL
                     SSSDNVLDSNVMINCSLMMYGNEDTEYLNYIYSNNTVNGKPVYYIFGRKRITVPDDAG
                     EVILISSDHCTIKNLNLDKGTIGILLAYSSHNIIQGNSITNQSMIAIDLGSADNNNNI
                     IQGNNIQGNGYGIDLEYSQGNTIKQNRIFSNAYGIFLSNTLNAVIRRNTISRNYYGIS
                     ATEANRSTIYFNNIYQNYAYGLSAESCAVTARWNWWGAIAGPNANGNGDHLSIKTDGQ
                     ITYAPWLRLPVLFTGALRFQFINKLQPSLTDTTVKTSGTTSFESPLTGFYQMDLFGLK
                     NIDNETTCVPPKTVMEHYFDM"
     gene            complement(6415..8220)
                     /locus_tag="VN377_03290"
     CDS             complement(6415..8220)
                     /locus_tag="VN377_03290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWR27444.1"
                     /translation="MTLDEKIQKGSGKLVGWHDPDENREWVVQNKPRGLVDKRMTVAQ
                     AVSDFIPDGSLLAMGGFGHVRVSMAVVYEIIRQRKQHLLLAGKTGVHDLDLLVGSGCV
                     DQVEVAYSFGHELRGLSPASRRAVETGKCKVTGEISNAGFQWRFLAAMMGMPFIPTRV
                     MLGTDTFAHSSAQTMVDPFSGKQICLLPACYPDVAVIHVPRCDRYGNAQIDGILVEDF
                     ELSRAARHLIVTTEKIVDSSKIRKEPWKTVIPFYLVSAVVETPFGSHPCQMPYQYFFD
                     EDHIGEWLSESVTDEGVQRYLEKYVYGLKDFSEYLKCVGGEKKMAQLARIERFEEPMT
                     APWLKAKNERPTKNELYSSTELLACVASRMLQDNKSVFVGTGLPMIAAMLAQRTHAPN
                     LLLFFEAGGIGPEMPVLPISVGDSRTFYSAVAASSMHDSMAMAQAGYIDYGFLGGAQI
                     DQFGNLNTTVIGPYEHPKVRLPGSGGANDVGTLCHQTIILMRQDKHRFLEKLDFLTTP
                     GYLSGFDSREAAGLPKGSGPYRVITQLGVYGFDPQSKQMMLLSLHPGVTVEQIIQNSG
                     FAIRVPEHVSVTTPPSQKELRVLKKIDPAGMVIGK"
     gene            8372..>8895
                     /locus_tag="VN377_03295"
     CDS             8372..>8895
                     /locus_tag="VN377_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWR27445.1"
                     /translation="MDFQLTDQQKLFKKTVREFAEKEIKPLAPKIDKEEYFPQELYKK
                     MGKMGLMSMTVPKRYGGAGIDKICYMIALEEISRVCGSTGITVEAHNSLGVGHIYEKG
                     TEEQRKKYLPNLLHGDAIAAWALTEPNAGSDAASLQTTAILEGDDWVLNGTKQFITSG
                     DIAAVTTVMAKTDKT"
BASE COUNT         2474 a         2199 c         1890 g         2332 t
ORIGIN      
        1 ccactcggtc caatatcact tgtgactgcc gatcgagttt ttttaaccac ttatcatcaa
       61 gaagttcatc agaagacatt aatttcctct ggcagcattt ttcttctgct cagactactc
      121 gcctgagtcc tactgtaacg aaatacgata tctgctttct cattttggat atcccatggt
      181 ttaataatta gaaggtcacc agcgcgcacc cgagctttgc gtttcatctt tccagggata
      241 cgagccatcc gagatttttt atcctcgcag acgacatgaa tacgagaccc gccaaggagc
      301 tgatccgcaa tcgcaaacag ctcattgttt tttcgattcg gtaacggtaa ccgatagtat
      361 ccatcctcgc cgtcttgagg tgaatgtgaa tgatgtgcct ttgaatacgt attcccaggt
      421 ggtgtcatac tttaaagccc caatgcatca gagattctct taagcgagtc caaactcagc
      481 tcaagaaaca tctgaagttc tagttccata tcctcacaca gttcaattct ttttcggtta
      541 catcctgctg cgaaggattt atccttaaac ttccccagca aggtcttcgg agtgacatca
      601 gcaagctttt tggtcggcat cacaagagcc gctgcgatga tcagaccgga caccgcatca
      661 gcagcaatca acgatttatc caggtaggtc tgagggattt gcgcggtgta ttgatagtta
      721 tgagccttga tagcgttaat agcctccggg ggtaagagat cattcaatac tttaatggtg
      781 accagtgaat gcttctccgg gtcgtttttt gtaaaatcat aatccaggtc atgcaacaat
      841 ccggtgagcc cccaaagctg cgggtcctcc ccaagcttcc ttgccatatc ctcaaggatt
      901 gcttcgacag caaaggaatg tttcatgagg ttttccgtac gaaggttcct cttaagaagc
      961 ggtacagctt ccgcccgggt cagcatatga aaaaggagta aagatggagt tgatttaaac
     1021 tttccccaga ccgtatcaaa agatttttaa ataagcaatc tggtttctcc gttgctggtg
     1081 catgtattaa aaccatcatg atcgttgatg aggaagttga ttttctcaaa caagtcaaaa
     1141 ccgtattaga aaaggaagac attgaggtcg taacagcacg gaatagcagg gaggcacttg
     1201 agcggctgaa agaggagaac gaagaaacct ttgatttaat cctggtgaac accaggatgc
     1261 cgggtagtac actgaccaat gcgcttttct ccatgaggcc tgcattgaaa aaactcccag
     1321 gcggtattga gaatttctta caaaaaccat tcacgaaaga agaactggtt gaatttatca
     1381 aagaaaaaat agacctccat taaaggagaa aagaaaaaaa gagtagcagg atcatttttt
     1441 ttaatgctat ttgagggtct ttacccattc atagactggc gtgacaagat ccttaatccg
     1501 ctgcttttca ggattcatcg gtgatggtgt ccagaaatcc gctggctcaa aatgaaaatc
     1561 agtgaccgtt ccgatgcgtt gctgttttgg tttaaacact attttcaatg gcatgccaaa
     1621 ctccacgtcc cacgggttca tcccatggag ctcgtttgcc atggcgactt tactggtacc
     1681 aatattcgcg tacaccagga taatcgggag acgtttcaat gatgatcgtc cactccatcc
     1741 cgcaatggtc caggtgtgca ccttcgcggt cagcggcatc tccacccatt cggttttttg
     1801 taaatgacaa tccaaattcc aacaatgggt gcggactgga acccaggagt caccacattt
     1861 cgggcaccgg gtccccatga ttttaccatc aagcaacccg atgaagaact tgctgttcca
     1921 cccatgtcgt atcgtgtaca gctgatcata atggaggaac tggttcatta cggtccggtc
     1981 ctctgagatc tcatggccga caaacaccag gcctgtttct ttcacctctg aaggtcggta
     2041 gtcttttttc tcggtgaaat tgggggtgtc agcgaatttt tcttttttgt ttttcatcac
     2101 aacagccacc gtcatcgtct aatgcctcct caggataaaa ttcgatatac tcgtcccggt
     2161 ccccgcatga ctgttgacaa tcccacattc cgcgtctgga acctcggttt tcttactgag
     2221 atgtttcttg ggaatcaatc ctgcgagctg ataataagat tcaaccatgc tcatcagacc
     2281 tgtcgcacca acaggatgac catacccgag taaccctcca ccaggattca tcgggatatc
     2341 accattcata ccggttcgtt catcccggat gaatttcgcg atcgtgtctt cattggccag
     2401 ccccaatgct ttgtagattt gcgcctctga ggtggagaac gcatcgtgga tctcaccaaa
     2461 atcaatatcc ttcagagggt ctttgatccc tgccatttta tacgcatgtt cagcagagtt
     2521 ccgacacgct gcaaactcag tcatccctgg gtacccaggg ccatagcgtt cgctccaacc
     2581 aggattgttc aatcgatccc ctgctcgaat cgtgcagctg gacaatccca ttccgtcaac
     2641 cttgacgtat cgatctgggt tgactttctt ggcgtactcc tcactgcaga ccaggagaaa
     2701 cgcgacgcct ctgctcatca gacagcaatc gtatttctta atgggatacg cgatgagcct
     2761 ggagttcatg acatcatcaa ccgtgatgta ccgatggtca tttcgatacc attgcgcctt
     2821 aggattatcc atcgcattat tcttgttttt gacagcgatg gccgcgacat cttcctcggt
     2881 gacatggttt tccttcatcc atttgttcgc catcgcagca tagtaaatag ggtagatgcc
     2941 tccgaccaaa agctcgtatc tgatatctga tgccatcgcg atgaactcgc tgccttgtga
     3001 ttgcgagaca gaatccatcg tctcccagcc gactatcccg acgacatcat gatgaccaga
     3061 caagacagca agtgctgcag cgtagagtgc ggagcctccg gtgtttcccc cgttctctac
     3121 tcggtagtgc ggtagaccga cgagtccaag atgatcatgg atgatccatt ctggtgcgag
     3181 ttgatggctg aaatggacgg agaaatggga gtaaatcata aggtcaaggt cacggagtga
     3241 gaacgctggg acatcctgca ttgtctcctt gatgcattct gctgctccgc cttcgatgct
     3301 ttcaaaacca gttgggccgt agtcaaatgg tgtggttgct cctccgatga tacaaacttt
     3361 tcgtgacatg actgactttc ccctcaggac tttcaacctt taaaggcccc agtcccctga
     3421 gagagggtat cgtttgagaa ttaaaaaagg ttgttatttt ttaaaaaata aatatcaagg
     3481 aaattacatg tcaaaatagt gttccatgac ggtttttggt ggaacacatg ttgtttcatt
     3541 atcgatgttt ttcaacccaa acaaatccat ttgataaaaa ccggtgagag gtgattcgaa
     3601 agaggttgtc cctgaggttt tcactgtggt gtccgtcaga gaaggttgaa gcttatttat
     3661 gaactgaaag cggagtgccc cggtaaagag aaccgggaga cgcagccatg gggcatacgt
     3721 gatctgtcca tccgttttga tactgagatg gtctccattt ccgtttgcat ttgggccagc
     3781 gatagccccc caccagttcc atcgggcagt aactgcacag gattctgcgg agagcccata
     3841 ggcgtaattc tgatagatgt tgttgaagta aattgtactc ctgtttgcct ccgtcgcaga
     3901 tataccataa taattcctcg aaatggtatt tctccggatg acggcattga gtgtattgga
     3961 tagaaagata ccgtacgcat tcgaaaaaat cctattttgt tttatggtgt tgccctggga
     4021 atattccagg tcgatgccgt agccattacc ctggatgttg tttccttgaa tgatattgtt
     4081 attgttgtcg gccgacccca gatcgatggc gatcatgctt tggttcgtga tgctgtttcc
     4141 ctggatgatg ttgtgtgatg agtacgcaag cagtatcccg atcgtccctt tatccagatt
     4201 gagatttttg atcgtgcaat ggtctgaact tatcaggata acttcacctg catcgtcggg
     4261 tacggtaatc cgttttcgac cgaaaatgta gtacactggt tttccattga ccgtgttgtt
     4321 tgaataaata taattgaggt attcggtatc ttcattccca tacatcatga gactgcagtt
     4381 aatcatcaca tttgaatcaa gaacattatc actagatgag aggagataca gaccaaacac
     4441 actggtatta caaatggtat tattgattaa tatattattt gaggatgtat caaaagacat
     4501 gccataggag ccgtcttgga ccgtgttatc gattatcgaa tttttccagc aggaatcaaa
     4561 ggaaaaaccc atcccattat caaccaatat attgtcttgg aaaatgttca tgtttgatga
     4621 atcgacatca atcccgtagt tattcccttg gatgatgtta ccactgatac tgttttcatt
     4681 gttatctgaa ccatccaaat caatcgcaac aacgctgttg ttagtaagga tgttgtcttc
     4741 gatggtgttc attgaacaat aggcaagttc caatccaaca ctcgcatcac tcaagttaca
     4801 ctgtgagacg atgcaaccgt tacaattagt gaggatgact tgtccagcgt cagaggggat
     4861 tgtcctattg gtttcgttta tataataata cacgggattc ccattcaccg tgttgcttgc
     4921 atcaacgtca tttcgataac cagcaggatc gtctcccgtg acctgtaaac cgcattgtat
     4981 catttcatta tccttcagaa cattatttcc ggatgcatca aacgaaatac cgacattgca
     5041 ttggtcaatg gtctgccccc ggagaatatt tcctgtgctc aaaccaaggt ttatccctgc
     5101 aagcgaaccg taatacgagc aattttccat agtgttgttt gatgaatcat agagaaacac
     5161 accatcccag aaactgtttt ggaacgtaca ggttttcacc gtgttatcct gagaggaaat
     5221 aagatagatc ccatgaccat cgttattacg aaaactgcag ttctctattt tggaatctgt
     5281 ggtacccgca aggagtatac cctcaaaatt atttgaaaaa ttacagtcgt tcaccgagat
     5341 attgtcacag ctgaccaatc caaggaagcc tatctctgtt gtcccattaa agggaagatc
     5401 gtgttgttca atgaggtagt acatcggttt tccattgatg gtatttgagg tgtcgatatc
     5461 gcagtaaaag tctgataccg atgatgaacc gataccaaag ttcgcgtaat tacccatcat
     5521 tgtgttattc cgcatttttg tatttggagc atagggtaag ctgatcccat cgtagccatc
     5581 ttgacagaca caaccagtga tttgattccc tgaggactgc tggataagaa tgctatcaaa
     5641 atgatttcta gtacaagtgc aatgaataat ttgattgtca tctgaggatt gaaagagcgt
     5701 aatagcatca gcattatcat aacacacaca attctggact atattttttc ttgagttcac
     5761 gaggtgaatg gcaacgagat tctcggacag ttcacaatca cgtatctggc aaaaggatgc
     5821 atcgactaag gagatgccga tccattcgtt cccattgatg atgcaatttg agagactggt
     5881 aaagttcccc ttaatcttca ccgccgcctg ataatagtac ccatgattat cggtgatacg
     5941 aaaaccatcg atggtgacat gatcggtgat aaccgcaaca gtatctaaag aattatcaag
     6001 ggaggaaccg ttgatttcag tactcgtttt atcttcaccg accagagtaa ttgatttgtc
     6061 gacgacgacg ttttcaagat acggggagga gtcatcgtac acaaaaacag tatcacccga
     6121 acttgaatcc ttaatagcat cctgaatcct tgaataattc ccttctccgc tcccaccaac
     6181 gtagaagaga gtcccatagg agacagactt gattgttttt tcaccgacgc tctgaccaaa
     6241 agatggaatg atacaaggga tgagcaagaa gagaataact ccaagcgcca attctttccg
     6301 ggttaaataa tatcgttgca caactttccc cttatctatc aatttataag tatctccccc
     6361 tataataata ttattgagct gtagaaaagg aaaacaatgc gtctaaaaaa gatattattt
     6421 tccaatcacc ataccggcag gatcgatttt tttcagaacg cgtagttcct tctgagacgg
     6481 tggtgtagtg actgaaacat gctcaggaac acgaatcgca aacccagagt tctggataat
     6541 ctgctcaacc gtcacccctg gatgcaacga cagtagcatc atctgtttgg actgaggatc
     6601 aaaaccatac accccaagct gagtaatcac tcgataaggc ccgcttccct tcggaagccc
     6661 tgccgcttcc cgcgaatcaa aacctgagag gtacccaggc gtcgtcaaga aatcaagttt
     6721 ctcaaggaac cgatgtttat cctgccgcat cagaataatc gtctgatgac acaaggttcc
     6781 cacatcattc gccccaccac tccccggcag acgcaccttc ggatgctcat agggcccaat
     6841 cacggtcgta ttcagattcc caaactgatc aatctgggct ccaccaagga acccataatc
     6901 gatgtaccct gcttgcgcca tcgccataga atcatgcatg ctagaagcag cgaccgcgga
     6961 ataaaacgtc cgtgaatcac caacggagat cggaagcacc ggcatctccg ggccgatccc
     7021 acccgcctca aaaaacaaca gcaggttagg cgcatgggtc cgctgcgcaa gcatcgcagc
     7081 gatcatcgga agaccggtcc caacaaacac agatttatta tcctgaagca tacgcgatgc
     7141 gacacaggcg agcagctcgg tcgacgaata cagctcattt ttcgttggtc tctcgttttt
     7201 cgctttcaac catggcgcgg tcatcggctc ctcgaaccgc tcaatccgag caagctgcgc
     7261 catcttcttt tccccaccga cacacttcaa atactctgaa aaatccttca acccgtacac
     7321 atatttctca agataccgct gtacaccctc atcggtcaca gactccgaaa gccattcacc
     7381 gatatgatcc tcgtcgaaga aatactgata cggcatctga caggggtgcg acccaaacgg
     7441 cgtctccacc acagcactca ccaaataaaa aggaataacg gtcttccacg gttccttccg
     7501 tatcttagaa ctatcaacaa tcttctcagt agtcacaatc agatgacgtg ccgcccggga
     7561 cagctcgaaa tcctcaacaa gaatcccatc gatctgcgcg ttcccatacc tatcacaccg
     7621 aggcacatga atcactgcga catccgggta acacgcaggg agaaggcaaa tctgtttccc
     7681 actgaacgga tcaaccatgg tctgcgcaga gctatgagca aacgtatcgg tccctagcat
     7741 cacccgcgtt ggtataaacg gcatacccat catcgccgca agaaaacgcc attgaaaccc
     7801 tgcgttactg atctcaccgg tcaccttgca ttttccggtt tcgaccgcac gccgagacgc
     7861 aggagacaac ccgcgcaact catgaccaaa tgagtacgcg acctcgacct gatccacaca
     7921 accagagccg accaacaagt caaggtcatg cactccggtc ttcccagcca gtagtaaatg
     7981 ctgtttccgc tgtctgataa tctcatagac gactgccata gagacccgca catgcccaaa
     8041 cccccccatg gcaagcaacg aaccatcagg gataaagtct gatacggctt gcgctacagt
     8101 catccgctta tccaccaaac cccgcggttt attctgaaca acccactcac ggttctcatc
     8161 aggatcatgc caccctacaa gcttcccaga gcccttctga atcttctcat ccaaggtcat
     8221 aagaaccatt ccccgacaca accatctgaa ccattaagat aaacgcctta aaaccaccta
     8281 gtatccacat cgagtatccc tacaaaaaat cttcgaccat acagaagcgt aaatttaaag
     8341 acttgtgacc tatcctggta tgaggatacc tatggatttc caacttaccg accagcaaaa
     8401 attattcaaa aaaacagtca gagagtttgc tgaaaaagaa attaaaccac ttgccccaaa
     8461 aatcgacaaa gaagaatatt tcccccagga attgtacaaa aaaatgggaa aaatgggact
     8521 catgagtatg acggtgccaa agagatacgg gggtgcaggt attgataaaa tctgctatat
     8581 gatcgccttg gaagaaatat ccagggtctg tgggtctaca ggcatcaccg tcgaagcgca
     8641 taacagctta ggtgtcggtc acatctatga aaaagggaca gaggaacaac ggaaaaaata
     8701 cctccccaat ctgctccatg gagatgcgat tgcagcctgg gcactcaccg aaccaaacgc
     8761 aggctccgat gccgcatcgc tacaaaccac ggcgatcctc gaaggagacg actgggtgtt
     8821 aaatggaacg aaacaattca tcaccagcgg ggatatcgct gcagtgacga cagtcatggc
     8881 aaaaacagac aaaac
//
LOCUS       DATMXJ010000119         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_12944, whole genome shotgun sequence.
ACCESSION   DATMXJ010000119 DATMXJ010000000
VERSION     DATMXJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12944"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..>3050
                     /locus_tag="VN377_03300"
     CDS             401..>3050
                     /locus_tag="VN377_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27446.1"
                     /translation="MTALTTATTVSNQSPYQIDSDHMSYTFTFKEPIIQSINADNTLY
                     STIQMQGCLNLGKQAGEPALPVKFIQLLLPYGTTVASVDVQGTPVQYNTQGINLIAQP
                     ILPYQNEVPIGEEIPQEFLIDNDLYASNQLYPTAHYGQYHIGYSHGYAIFDISLSPTQ
                     YIPSAGQVFYYPKMTLTVHLEQSDDMNTLFRNSAADEQWVQSLVSNPEMTFTYLGAPT
                     FEYPGGLCDPSESFDYVIITTTQNGLDYWATGGTTPYNWESLMDHHAAEGLSCTLVTA
                     EDIDACVDYQGSAPFNDRQAHIREFCKDAYEDWGTSYILIGADGESNYIPARDMDSSY
                     ESDIDADIYWSNLDNNFNANSNSYWGEEGDLGFDLYSELFLGRLTCDEPQDVSNWMTK
                     SFYYAQSTDYDYIENAAFFAGTMGWSAQGDDYIDFSAIKGTSNWLGPDPDEHGVYPTW
                     LGFQYGFETWNAVNPGNLFNLSIKYTSENPNPGWEGGDAIGKFRTAINNDRVTIISGV
                     AHADPTMSLDVYDSEWEGNYTNTKPFFITDFGCHCGDFDNADDGVLHSMLFHSDTELA
                     FACVYNTCYGWGSWTSTNSSSAIQMKSFWDYFLDMENNSQSYANWQFGKAQAWSKDMM
                     APTVNWTWTSAPGSYRAVIQGCTYFGDPAQQFKSPSPSDPPARPTKPVGQTLGIWNVE
                     YTYTSTTSDPNADEIYYLFDWDDGTSSGWIGPYASGVTGIASHIWTELGTYDVKVKAR
                     DVWGAGSLWSESLTVTITDNNPPNTPTVTGPGEGKPGNSYLFNILSDDLDGHNIYYFV
                     DWGDNTTSGWFGPYVSGTQIHLTHTWTAVGTYTVKAKAKDTLDAESNWGTLQVVMPLE
                     YKYSFTVFLQHLLEKFPNMFPILRHLL"
BASE COUNT          897 a          689 c          648 g          816 t
ORIGIN      
        1 tatttaagga tgaaaagatg ttgaaacatc atcagttttt ccgtagtttc tatctatatg
       61 gaatttaatg tttttgacct aggattcctt atattattga atccttatac taagatagct
      121 attgaatttt gaaagatgat ctagacgagt aagccgaaag ctgatagagt agcatgattg
      181 gaagatcgaa gagtctatcg tgctcattcg aagaacggtg gtttcatata atttttataa
      241 tcttatttta atattaatta ttgttttcca attttcatca aaagttttaa taatgccagc
      301 gacattattt ttcaatggag gtatactaaa aatgaatcta ttgaataata tattaaaaag
      361 taaaaagcag gtatcagtag ttctctgcat tctatttacg atgactgcat taactacagc
      421 aacaaccgtc tcgaaccaga gtccctatca gatcgatagc gaccatatga gctacacatt
      481 tacctttaag gaaccgatca tacaatcgat caacgcggac aatacattat attcgacaat
      541 ccaaatgcaa ggatgtctta atttaggaaa acaagcaggc gaaccagcat tgccggtgaa
      601 atttatccag cttctcctgc cgtacggaac cacggttgcg tcagtagatg tgcaaggaac
      661 acctgtgcaa tataatactc aaggtattaa tctgatcgca cagccaattc ttccttatca
      721 aaacgaagta ccaatcggag aagaaatccc acaagaattc ctcatcgata acgacctcta
      781 tgcatccaat caactttacc caacagcaca ctatggtcaa taccatattg gatactctca
      841 tgggtatgcg atttttgata tctcactttc accaacacaa tatatcccct cggcaggaca
      901 agtcttttac tacccaaaaa tgacgctcac tgtccatctt gaacaaagtg atgatatgaa
      961 caccttgttc cggaacagtg ctgcagatga acaatgggtc caatcacttg tcagtaaccc
     1021 agaaatgaca tttacctacc ttggtgcgcc aacctttgaa tatcccggag gattgtgcga
     1081 tccatcagag agtttcgatt atgtgatcat tacaaccacc cagaatgggc ttgactattg
     1141 ggctactggt ggtactactc cctacaactg ggaaagcctc atggatcatc atgctgcaga
     1201 aggattatcc tgcactcttg tgaccgctga ggatatcgac gcatgtgttg attaccaggg
     1261 ctcagctccg ttcaatgaca gacaagccca catccgagaa ttctgcaagg atgcatacga
     1321 agactgggga acaagctaca ttctcattgg tgctgatggg gagtcgaatt acattcccgc
     1381 aagagatatg gattcaagtt atgaatctga tattgatgca gatatctact ggagcaatct
     1441 tgataataac ttcaacgcga attctaactc gtattggggt gaagaagggg accttggctt
     1501 cgatttgtac tcggaactct tccttggacg tctcacctgt gatgagcctc aggatgtttc
     1561 caactggatg acgaaaagct tctactatgc gcaaagcacc gattatgatt atattgagaa
     1621 tgcagcgttt tttgctggta caatgggttg gtccgcccaa ggagatgact acattgattt
     1681 tagtgcgata aaaggcacgt ctaattggct aggaccagat cccgatgaac atggtgttta
     1741 tccaacctgg cttggattcc aatatggatt tgagacctgg aatgcggtga accctgggaa
     1801 tctattcaac ctttcgataa aatatacatc ggaaaatcca aatcctggtt gggaaggtgg
     1861 cgatgcgata ggcaaattca gaaccgccat taacaatgac cgagtgacca tcattagcgg
     1921 tgtcgcccat gcagatccaa caatgtccct tgatgtctac gactcagaat gggagggtaa
     1981 ctataccaac acaaaaccat tcttcatcac tgatttcggc tgccactgcg gtgactttga
     2041 caatgcagac gatggtgttc ttcactctat gttattccac tctgataccg agcttgcgtt
     2101 tgcgtgcgtg tataacacct gttatggctg gggatcttgg acgagtacca attcctcaag
     2161 cgctattcag atgaaatcct tctgggatta cttcttagac atggagaaca actctcagag
     2221 ctatgcaaat tggcaattcg gcaaagcaca agcctggtca aaggatatga tggcgccaac
     2281 cgttaactgg acctggacct ctgcaccagg cagttatcga gcggttatcc aaggatgtac
     2341 ctactttggt gaccctgcgc agcaatttaa atccccatcc ccgagtgatc caccagccag
     2401 accaacaaaa ccagtcggtc aaacactagg gatctggaat gttgaatata cctacaccag
     2461 tactacctcc gacccgaatg cagatgagat ctactacctc ttcgattggg atgatggaac
     2521 gagcagcgga tggatcggac cgtatgcctc aggagtgaca ggtatcgcgt cgcacatctg
     2581 gactgaacta ggaacctacg atgtcaaagt aaaggcacgg gacgtctggg gtgctggtag
     2641 tctatggtca gaatcattga ccgttacgat caccgataac aatccaccga acacgccgac
     2701 ggttaccggt ccaggtgaag gaaaaccagg gaattcgtac ctgttcaata ttctctccga
     2761 cgatctcgat ggtcataata tctactattt cgttgactgg ggagacaata cgacatctgg
     2821 ttggtttggt ccttatgtct caggtacaca gatccatctg acccatacct ggactgcagt
     2881 aggaacatac acagtgaaag caaaagcaaa agacacgctg gatgcggaaa gcaactgggg
     2941 cacgcttcaa gtggtcatgc ctttggaata caaatactcc tttaccgtat tcctgcagca
     3001 cctcttggaa aaattcccca acatgtttcc catcctacga caccttttgg
//
LOCUS       DATMXJ010000120         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_44482, whole genome shotgun sequence.
ACCESSION   DATMXJ010000120 DATMXJ010000000
VERSION     DATMXJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44482"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VN377_03305"
     CDS             <1..1281
                     /locus_tag="VN377_03305"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27447.1"
                     /translation="RCYTYDVENNDWDSASNGLPTSGEYYPQFGDINGDGSLDIVGYV
                     GPTGYAYLGDGNGNWVQDGTFSMPSSGYFSAFLVDGDFDHDGREDVVIQAEQGSGYVY
                     VNLLKAFSPWLEPTSLTALVQSPHGGETFRSGSIRNIRWLSAVPASQGESTVDILLSR
                     HGVSGPWEPLATDIPNNGCYQWLIGASGSENCRIKVVVTTSQSTASAISGSDFTILGF
                     DVDAHGPYYGSPDQMIQFTGTAENGNPPYDYHWDFGDGNTSDQQNPTHSYSNEGNYTV
                     VLSVTDSDDLTVTDTTWALISGTNAPPNTPDIQGPSNGKPKVQIKYTVVATDPDLDIV
                     FYFIDWGDNTTSGWLGPYGSGEQKSVSHAWSYKGTYSVKVKAKDIHGAESDWGTLSVK
                     ILFSYDKPVIAFREWFFERFPNAFPLLRYLSEFM"
     gene            complement(1360..1839)
                     /gene="dcd"
                     /locus_tag="VN377_03310"
     CDS             complement(1360..1839)
                     /gene="dcd"
                     /locus_tag="VN377_03310"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWR27448.1"
                     /translation="MVILSDVDIEQALESNELGIEPFIQKNLTPNGYDLSIDEIYLRT
                     TDQHIKEGIAVIPPLTWFAISTKEFVKMGPQITSQLWIRSSYARRGIMASFGKVDAGF
                     HGTLTFGCFNASDKQVEIPIGDRFCQIVFEHLAQAPKALYHERSGTYQNQRGVTLKR"
     gene            complement(1858..2340)
                     /locus_tag="VN377_03315"
     CDS             complement(1858..2340)
                     /locus_tag="VN377_03315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWR27449.1"
                     /translation="MANRRAIFETTKGKFTFELYEDKAPLTTGNFIKLANQGFYNGLI
                     FHRVIPQFMIQGGCPEGTGTGGPGYTIKDEFHKDLSNVRGTIAMANRGPNTGGSQWFI
                     NIVDNCYLDFDKKPYSSAHPVFGKVVEGMEIVDAISQVKTSRNDKPAQDIIIKKITIS
                     "
BASE COUNT          615 a          575 c          555 g          739 t
ORIGIN      
        1 cgctgttata cctatgatgt ggaaaataac gactgggatt cagcatccaa tggtcttccg
       61 acatctgggg agtattaccc ccaatttggt gatattaacg gtgatggatc tctcgacatc
      121 gttggttatg ttggtccaac cgggtacgcg tatctcggtg atggaaacgg gaattgggtt
      181 caggacggga ctttttccat gccttcttct ggttatttct ctgcttttct cgtggatggg
      241 gattttgacc atgatggtcg cgaggatgtc gttattcagg ctgaacaagg cagtgggtat
      301 gtgtatgtca atctattgaa agcattttct ccctggcttg aaccaacgag tctcactgct
      361 ttggtacaat caccacatgg tggagaaacg ttcagaagcg gctccattcg gaatatccgg
      421 tggctttctg cggtccccgc atcacaaggg gaatccacgg tcgatattct cctttcgcgt
      481 catggtgttt ccggtccctg ggagcctctt gcgaccgaca ttcctaacaa tgggtgttat
      541 caatggttga tcggtgcaag tggctctgag aattgtcgta tcaaggtagt ggtgaccaca
      601 tcccaatcga ctgcctctgc gatttctggt tctgatttta ccattcttgg ttttgatgtc
      661 gatgcgcatg gtccgtacta tgggtcacct gatcaaatga ttcagtttac tgggactgcg
      721 gaaaatggga atcctcctta tgattatcac tgggattttg gtgatgggaa cacctcggat
      781 cagcaaaacc cgacacatag ttattccaat gaggggaact acacggtggt attatcggtc
      841 accgattcag atgaccttac cgtcacggat acgacctggg cgttaatcag tgggaccaat
      901 gctcctccga acacacctga tattcagggg ccttctaatg gaaaacccaa ggttcagatt
      961 aaatatactg ttgtggcgac tgaccctgat ttggatattg tcttctactt cattgattgg
     1021 ggggataaca cgacgagtgg atggcttgga ccatatggtt ctggtgaaca aaaatcagta
     1081 tctcatgcct ggagttacaa agggacgtac tctgtcaaag tgaaagcaaa ggacatccat
     1141 ggggctgaga gtgactgggg aacgctttct gtgaagatac ttttctcgta cgacaaacct
     1201 gttatcgctt tccgggaatg gtttttcgaa cgattcccaa atgcatttcc tttactgagg
     1261 tatctttcag aatttatgta atcacgtttc ttttctcttt ttataaaaat aacacaggaa
     1321 ataaatatat acaaatatat atctttgttt ggggaagaat taccgcttta acgtcacacc
     1381 acgctggttc tgatacgtcc cagaccgttc atgatacaac gctttcggtg cttgtgcgag
     1441 atgttcaaag acgatctgac aaaaccgatc accaatagga atctccactt gcttatcact
     1501 tgcattaaaa cacccgaagg tgagcgtccc atgaaaccca gcgtcgacct tcccgaaact
     1561 tgccatgatt cctcgccggg cataactgga acggatccat aactgcgacg ttatctgagg
     1621 acccattttg acaaattcct ttgtgctaat cgcaaaccag gtaagtggcg ggatgaccgc
     1681 aataccttcc ttgatatgtt gatcggtggt tcggagatag atttcatcaa tggaaagatc
     1741 atatccattc ggtgtcagat tcttctggat gaacggctct atcccaagtt cattactttc
     1801 aagagcctgc tcgatatcga catcagaaag aataaccata gaaaccacat catcttctta
     1861 tgatatcgta atttttttta tgatgatatc ctgagcaggt ttgtcgttcc tgctcgtttt
     1921 cacctgggag attgcatcga caatctccat gccttcgaca actttcccaa acacagggtg
     1981 cgcactcgaa tatggtttct tatcaaagtc aaggtaacag ttatccacga tattgataaa
     2041 ccattgactc ccaccggtgt tcggtccgcg gttcgccatc gcgatcgtcc cgcgtacgtt
     2101 gctcaaatcc ttatgaaact catcctttat cgtgtaccct ggaccgcctg taccagttcc
     2161 ttcagggcat ccaccctgga tcatgaactg gggaatcacc cgatgaaaga tcaacccatt
     2221 gtagaatcct tggttcgcca gtttaatgaa attccccgtg gtgagtggtg ctttatcctc
     2281 atacagttca aaggtaaatt tacctttggt tgtctcaaaa atcgctcttc tattcgccat
     2341 tttcttcgta tctcctcgtt tatcagctta atatgataat attattgatg actatatcta
     2401 cgtccggttt accagtccca tcaccgctat cgggatgagg ttgcgctgcg attgttcgta
     2461 cgacatcaat accttcgatc acta
//
LOCUS       DATMXJ010000121         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_2466, whole genome shotgun sequence.
ACCESSION   DATMXJ010000121 DATMXJ010000000
VERSION     DATMXJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VN377_03320"
     CDS             complement(<1..1487)
                     /locus_tag="VN377_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27450.1"
                     /translation="MKKILPILLVGVLLISGFGAAVSVNTQKNQLFLQQGVSFSEPVL
                     TDHDNYLSVEIAEATSLASSPGNPMLPLYTKLFSFPFGTIIKDVSFTASSEQIIRLTK
                     EILPGTQPTPVSGGLMTSSNQKQVKNEAVYSGKAMYPASRLEYQVGAGLQGNEHVTFL
                     SVLYYPLLYQPQQYTLLFTPNAQITITYEEPQQSKPLSTETKLLIIAPEEFSAALQPL
                     IDHKNQYNVSTTLITTEWVADHSTGRDLPEQIKYAIKDEIETNAITSVLLVGSFDKLP
                     MRRSYVSLWGDWDEEMITDLYYSDIYDGSGNFCSWDSNNNDQFGEASDHVDLFPDVHL
                     GRLACDTLQDVTIVVDKIIHYETETYGSDWFNTMIYIGGNTFRWSPGNDGEEINQIIM
                     GIMPQFSPETIWTSKGNFNRRTISNAITAGAGFLDYSGHGFENGMGTYTPAGVSMKYY
                     YSAFIKDTQNGYKLPIIFFDACLTAKIDYVLQDLLDYRSYAVFNV"
     gene            1612..>3735
                     /gene="leuS"
                     /locus_tag="VN377_03325"
     CDS             1612..>3735
                     /gene="leuS"
                     /locus_tag="VN377_03325"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF008957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWR27451.1"
                     /translation="MSDQILPMEENTYDYIAVEQKWQNRWFKENINLATKEKQKKFFI
                     HFAYPGVSGYLHVGHMRGFTYCDVIARYKRMTGYDVLFPAGFHASGIPSVGFAKKVER
                     QDQDTLKLLKQNGCSEECIKKLTDPIEVVNYFSTIYVDEYWKRFGYLIDYTRVMNTIS
                     EGYKKFIQWQFHQLHEKNLLIQKPHYAPYCPSCGPVAVDKSETDVSQGGSAEILDFTV
                     LKFTLKDGTILPAATLRPETIFGVTNMWVNPTVEYHFVKVNNETWICSEECIKKLSYQ
                     QDLVQPLKKTIKGKDLIGKTCIVPEINREVPILAGLFADPAVATGIVMSVPAHAPYDW
                     IALVESKAPIEAIKIIDVKGFGNNPAKEACDQLGIKTQTETDKLNEATDLVYKKEFHT
                     GILNERCGKYAGIKISEIKDTVKNDLIQRNLATTLKEFSEAVICRCKEKVMIKQIPDQ
                     WFIKYSDEALTQESKDAITTMNIYPQEYKDDLPKILDWFGDRAAIRRGSWLGTEFPYK
                     KDWIIEPISDSTLYPAYYVISPYVNQKKITVKEMTDEFFNYVFLGIGSPKHETWKSIK
                     SDFDYWYPVDINLGGKEHKTVHFPVYIMNHVAIMPEQKRPQGIFVHWWVTQKGKEKIS
                     KSKGGAEPIVEAAVTYGVDAMRLYYLHIGSPFVDIEWDPETVLKYKNRIINIWKLIQQ
                     ISSRSDKKQENLDNWLKSTLQRRVQK"
BASE COUNT         1157 a          876 c          803 g          899 t
ORIGIN      
        1 aagacattaa acacagcata cgatcggtag tctagaagat cctggaggac ataatcgatt
       61 tttgcggtca gacaggcatc aaaaaatatg atgggaagct tatatccatt ctgggtatcc
      121 ttgataaacg cggaataata atatttcatc gaaacaccag ctggagtgta ggtgcccatc
      181 ccattttcaa acccatgacc agaatagtca aggaacccgg caccagcggt gatggcattt
      241 gatattgttc ttcgattgaa attacctttc gatgtccaga tggtctctgg tgagaactgc
      301 ggcatgatac ccatgatgat ttggttgatc tcttccccgt cgtttccagg ggaccagcgg
      361 aaggtgtttc ccccgatgta aatcatcgtg ttgaaccagt cgctcccata ggtttccgtc
      421 tcatagtgga tgatcttgtc tacgacgatg gtcacatcct ggagagtatc acaggcgagt
      481 cgaccaagat gcacatcagg gaaaagatcg acatggtcgc ttgcctcacc aaattgatca
      541 ttgttgttcg aatcccagct gcagaaattc ccagaaccat catagatgtc agaatagtag
      601 agatccgtga tcatttcttc atcccagtcg ccccagagac tcacgtagct gcgtcgcatc
      661 gggagtttat cgaaactacc aacaagtaaa accgaggtga tcgcatttgt ctcaatctca
      721 tcctttattg catattttat ctgctctggt aaatctcggc ctgtcgaatg gtcagcgacc
      781 cattctgttg tgatcagagt cgtcgacaca ttatattgat ttttatggtc aatgagcggt
      841 tggagcgcag cagagaattc ctcaggggcg atgatcagga gcttcgtctc tgtagaaaga
      901 ggtttagatt gttgtggttc ttcataggtt atggtgattt gtgcatttgg agtgaaaagt
      961 aacgtgtact gttgcggttg atagagaagc ggataatata gaactgagag gaaagtcaca
     1021 tgttcgtttc cctgtaaacc agcaccgacc tgatactcca agcgagacgc tggatacatg
     1081 gctttccctg aatacaccgc ctcgtttttc acctgttttt gatttgaaga agtcatcaaa
     1141 ccaccagaga caggcgttgg ctgagtaccg ggaaggatct ctttcgtgag tcgaatgatc
     1201 tgttcagaag acgcagtaaa cgagacatct tttatgatag taccaaacgg aaaagaaaac
     1261 aactttgtat acagtggtaa catcggattt ccaggtgatg atgccaaaga ggttgcttcg
     1321 gcgatctcga ccgatagata attgtcatga tccgtgagta ccggttctga aaaagaaaca
     1381 ccttgctgaa ggaataattg gttcttttgc gtattcacag atacagctgc tccaaaacca
     1441 cttattaata acactcccac taggagaata ggtagtattt tcttcatatt tttccccatt
     1501 attacgcttt tatagaactc ttatagagct aatggtattt aaattttctt tactataatt
     1561 atagacaaag accaaggaag acgacacgaa atattatata aaacaaacct tatgtccgac
     1621 cagatactac ccatggaaga aaacacctac gattatatcg ctgtcgaaca gaaatggcaa
     1681 aacagatggt tcaaggaaaa catcaattta gcgaccaaag aaaaacaaaa aaaattcttc
     1741 attcatttcg catacccggg ggtctcaggg tacctccatg tcggtcatat gcgtggattt
     1801 acctactgcg acgtcatcgc acgatataaa cggatgaccg gctatgatgt gctgttcccc
     1861 gccgggtttc acgcatcagg tatcccttcg gtcggttttg caaaaaaggt agaacgacaa
     1921 gaccaagaca cccttaaact gttaaaacaa aacggatgct cagaagaatg catcaaaaaa
     1981 ctcaccgatc ccatcgaggt cgtcaactac ttcagcacca tttacgtcga tgagtactgg
     2041 aaacggtttg ggtacctcat cgactacacc agagtcatga acaccatctc cgagggatat
     2101 aagaaattca tccaatggca attccatcaa ctccatgaaa aaaaccttct catacagaaa
     2161 ccacattatg caccctactg ccccagctgc ggacctgttg ctgtcgataa atcagaaacc
     2221 gatgtctctc aagggggatc agccgagatc ctcgatttca cggtcctcaa attcaccctc
     2281 aaagatggga cgattcttcc tgcagcaacc ctcagaccag agaccatctt tggtgtcacc
     2341 aacatgtggg tgaatcccac ggtcgagtat cattttgtga aagtaaacaa cgaaacatgg
     2401 atctgctcag aggaatgcat taaaaaactc tcctatcaac aggacttggt tcaaccctta
     2461 aagaaaacca tcaaaggaaa agacctcatc ggaaaaacct gcatcgtccc agagatcaac
     2521 agagaagtac cgatccttgc cggcctgttc gcagaccctg ctgtcgccac cggtatcgtc
     2581 atgtcagtac cagcccatgc gccctacgac tggatagcgc tcgtcgaatc aaaagcacct
     2641 atcgaggcaa tcaaaatcat cgatgtcaaa ggatttggta acaaccctgc gaaagaagcg
     2701 tgcgatcagc ttggcataaa aactcagaca gaaacagata aactcaatga agcaaccgac
     2761 cttgtctaca aaaaagagtt ccacaccggg atactcaacg aaagatgtgg taagtacgcg
     2821 gggattaaaa tctctgagat aaaagacacg gtgaaaaacg acctcatcca gaggaacctc
     2881 gccaccacct tgaaagagtt ctcagaagca gtcatctgcc gctgcaaaga aaaagtcatg
     2941 ataaaacaga tccctgacca atggttcatc aaatacagtg acgaagcact tacgcaagaa
     3001 tcaaaagacg cgatcacaac catgaacatc tacccacagg aatataaaga cgatctgcca
     3061 aaaatccttg attggttcgg agaccgggct gcgattcgac gaggatcctg gcttggcaca
     3121 gagttcccct acaaaaaaga ctggatcatc gaaccgatct ctgattcaac gctctaccca
     3181 gcttactatg tgatttctcc gtatgtgaac cagaaaaaga tcacagtaaa ggagatgacc
     3241 gatgagttct tcaactacgt gttcctcggg attggatccc cgaaacatga gacctggaag
     3301 agcatcaaat ctgattttga ttattggtac cccgtggaca tcaaccttgg cggaaaagag
     3361 cataagacag tccatttccc tgtttatatc atgaaccatg tggcgatcat gccggaacag
     3421 aaacgcccac aagggatctt tgttcactgg tgggtgaccc aaaaaggaaa agaaaaaatc
     3481 agtaaatcca agggtggagc agaaccgatc gtcgaggcag cggtcaccta cggcgtcgat
     3541 gcgatgcgcc tgtattatct tcatatcggc tccccgtttg tcgatatcga atgggacccg
     3601 gaaaccgtcc tgaaatacaa aaaccgaatc atcaacatct ggaaactcat ccagcagatc
     3661 tcatcacggt cagataagaa acaagaaaat cttgacaact ggttaaaaag cactctccaa
     3721 cggcgagtac agaaa
//
LOCUS       DATMXJ010000122         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28098, whole genome shotgun sequence.
ACCESSION   DATMXJ010000122 DATMXJ010000000
VERSION     DATMXJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28098"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VN377_03330"
     CDS             <1..368
                     /locus_tag="VN377_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27452.1"
                     /translation="GREHRVWLPETIGSSDDLRTPSSTGKSDVSLHHWCTLCGCIQNI
                     SDDRPKKIGYWINVLSKLAREYHLTDCQRRLVIKALQSNEFFEDLYATTGSSQQGVFV
                     KLLQNYSNLDRNTIDSYIF"
     gene            457..1356
                     /locus_tag="VN377_03335"
     CDS             457..1356
                     /locus_tag="VN377_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWR27453.1"
                     /translation="MGNGMKRTLVVIPCFNEEATIGSVVLKAKQYVDEVVVVDDGSTD
                     KTAKIAEYAGATVLRHDVNRGYGAALKSGFEYARSNDFDVMTILDGDGQHDADQIQTV
                     MMPVSDGKADICVGSRFLDSTGNMVPMYRHFGIAVLTKFTNAGSEKENRVTDGQSGFR
                     SYSRKAIENIDLRDPQMGASAEILLQARSMHLTSKEVPITCSYEGDCSTEKPVGHGLG
                     VLISIFKYLEVKHSLLFFGVPGLIMFAAGLAMGTFVYFEYLANKVVPIGTALVTVMLL
                     VLGMLAGITGLILHAVINANQRR"
     gene            1555..3342
                     /locus_tag="VN377_03340"
     CDS             1555..3342
                     /locus_tag="VN377_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27454.1"
                     /translation="MPVEFVELTKLIGGIILLIVPGYLWSYFLFKDHRHIERIAFGFV
                     LSLGVLCSGMFVIDVLLGLPLTVLKVFLLLVVYAASSVVVFFYTIYKSGLPKLHPSSL
                     KNPKVLLLLGILTFTAFMAFLPHLSNSYYLPFHVDEWIHWEYSRAVMEQGSSVFINPY
                     TGSGTTRSLESGFHYITASLKWITGSSFNTIVVFMPAVIMLFMSLIAFVIGERSERKF
                     GLEAAFLVAFIPTTCRMLGPSFYVAVAMGLLFTVFIIWLLQLKKTLATLLIPVFIWCV
                     LLIHLPTALACIIIMFLYGFVLLLEKEYKFSVLTMGLSLIPIGLFFLFASRLEYGLQQ
                     LIDAFFGGKTFFFDYDLPQIWPSFEHLGFITWILCIIGAYVAFTKGKAIHRTFTLSAI
                     AFIVLIGLYDKMSYGLPIMYERSFMYLFLMVTLLAGYGLSELRQFITNHSEHLISKRY
                     KQLLRHRGLLFTVVVCIILLVSVVPAHLGIQYYQMISEDDYATFTWMNDHLDEYRNET
                     HLYDRAAVNPFYASPFSAVTGLYIVSSSMHPIYGYELHTQMETFLNDKCVDTGFLDDH
                     KISVIYGDCNNNNLTMIYPDVYLYPGLKK"
     gene            3383..3676
                     /locus_tag="VN377_03345"
     CDS             3383..3676
                     /locus_tag="VN377_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HWR27455.1"
                     /translation="MTIVVGTRPEIIKMSPIIRECKRRLDYFILHMSQHYSYNMDQVF
                     FEQLELTMPKYNLYVGSGTQTEQYGRALIGIEQMLLKEQPAVVLVGGDINLHR"
     gene            3678..>4376
                     /locus_tag="VN377_03350"
     CDS             3678..>4376
                     /locus_tag="VN377_03350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWR27456.1"
                     /translation="MSVVIPVKNEEHKISKCLEAVINQTLSPYEIIVVDGHSIDDTVK
                     KAQSFSVKILYEDYRTRAGACHVGLKNATGEYIAFTDADCIPRENWLEAELKVIKKHR
                     NVAGVGCCIDNISKGVWEETINEISKTFIGSGRSIQGRQFTKEKKVLSISGCHALYRK
                     KMLEDVGGFNVRLKTCEDTEINKRLRKKGYDLIYSPKTSVLHDHTRGTKLFAKRMFQY
                     GWGRAQSKLFDMQLL"
BASE COUNT         1229 a          830 c          972 g         1345 t
ORIGIN      
        1 gtgggagaga acatcgggtt tggctgccgg aaaccattgg ttcatccgat gatttgagga
       61 ccccgagtag tactggaaaa tcagacgttt cactgcatca ctggtgcact ttgtgcggat
      121 gcatacagaa tatctctgat gaccgcccga aaaaaatagg gtactggata aatgttctct
      181 cgaagcttgc gcgggaatat catctcactg actgccagag acgactcgtg attaaagcat
      241 tgcagtccaa tgagttcttt gaggacctgt acgcaaccac aggttcttca caacaggggg
      301 tatttgtaaa gctattacaa aactatagta atctcgacag aaacacgatt gattcatata
      361 tcttttagac tgacttgaca accttcgagg ttgggtgata caaggctaaa ggatgggtag
      421 tgtaagaaag gatgaattgg aggagcagag aaataaatgg gtaatggaat gaaacgcacg
      481 ctagtggtta ttccctgttt taatgaagag gcaacgatcg gcagtgttgt cctaaaagcg
      541 aaacaatatg ttgatgaggt tgttgtcgtc gatgatggga gcacagataa aaccgcaaag
      601 atagcggaat atgcaggtgc aacggttctc agacatgatg tgaacagagg atatggggct
      661 gctttgaaaa gcggttttga atacgctcgt tcaaacgact ttgatgtcat gacgatcctt
      721 gatggagatg gacagcatga cgcagatcaa attcagacgg tgatgatgcc tgtttcagat
      781 gggaaagcag atatctgtgt tggttctcgc ttccttgaca gtaccggcaa catggttccg
      841 atgtaccgtc attttggtat cgcagtgtta accaagttta ccaatgctgg ctctgagaaa
      901 gaaaataggg tgaccgatgg ccagagtggt tttcgctcct attcgcgaaa agccatcgaa
      961 aatatcgatc tgagagatcc tcagatgggg gcgtccgctg aaatcctcct gcaagctcga
     1021 agtatgcatc tcacaagcaa ggaggtcccc atcacgtgta gttacgaggg tgattgctcg
     1081 acagaaaaac cggttggtca cggccttggg gtccttattt ccatcttcaa atatttagaa
     1141 gttaagcatt ccttgttgtt ctttggtgtc cctggtctta tcatgtttgc cgcagggctt
     1201 gccatgggta cctttgtata ttttgagtac ctggcaaaca aagttgttcc aattggtact
     1261 gcgttagtga ccgtaatgct ccttgttctt ggaatgcttg caggtatcac cgggttgatc
     1321 ttacatgcgg tgatcaatgc aaaccaacga cgatagtaaa agttttttcc aaatggtcgg
     1381 tgttcttcct gtgtttcttg attttcttct ctttcgtttc cttgcgtgat acaatacgtg
     1441 aagcttggtt ggagagatac gattgataat tttatttgca aaccttgaag aatcatatgt
     1501 gagaggtgaa actattattt taaattgtaa aaaaacgaag gataaaggat tgtaatgcca
     1561 gtcgaatttg ttgagctcac aaaattaata ggtggcatta tcctcctcat cgttcctggg
     1621 tatctctgga gttatttcct ttttaaagat catagacata tagagagaat tgcctttgga
     1681 ttcgtcctta gtttaggagt attgtgcagt ggaatgtttg ttattgacgt gcttttaggc
     1741 cttcccttaa ccgttttaaa agtgtttctt cttcttgtag tctatgcggc gtctagtgtt
     1801 gtagtttttt tctataccat ctacaaatca ggtctgccaa aactgcatcc ctcttccctg
     1861 aaaaatccaa aagtcctgct gttactcggc atactgacgt ttactgcgtt catggctttt
     1921 ttgccccatc tctcgaacag ttactacctg ccgtttcatg tggatgaatg gatccactgg
     1981 gagtatagca gagcggtgat ggaacaggga tcatcggtgt ttattaaccc ctacacaggg
     2041 agtggtacga cccgatcact tgagtcaggg tttcattata ttaccgctag tctgaaatgg
     2101 atcaccggta gctcatttaa caccatcgtc gtgttcatgc cagctgttat catgttgttt
     2161 atgagtctta tcgcgtttgt tatcggggag cgatcagagc gaaaatttgg tcttgaagca
     2221 gcgttccttg tagcgtttat accgacaacc tgtcgaatgc ttggtccatc gttctatgtc
     2281 gccgtagcta tgggattgtt gttcactgtc tttattattt ggttgttaca gcttaagaaa
     2341 acgctcgcta cgctgctcat ccctgttttc atttggtgtg tattgctgat ccatctacct
     2401 actgctctcg cctgtatcat tatcatgttt ttatatgggt ttgtattact cctggaaaaa
     2461 gaatataaat tctctgttct taccatgggt ctttcattga ttcccatcgg cttattcttt
     2521 ctttttgcta gtcgattgga atatggtttg caacaattaa tcgatgcctt ttttggaggg
     2581 aaaactttct ttttcgatta tgatctccct cagatatggc cgtcgtttga acatcttgga
     2641 ttcatcacat ggatattatg tataatcggg gcatatgttg cttttaccaa aggaaaagcc
     2701 atccatcgta cgtttacctt gtccgccatt gcgttcattg tcttaatagg actgtatgac
     2761 aagatgagct atggactccc catcatgtac gaacggtcct tcatgtatct gtttttgatg
     2821 gttaccctcc ttgcaggata cggattgagt gagctacgac aattcataac caatcattcg
     2881 gaacacctca tatctaaacg gtacaaacaa ttattacgac atcgtggtct cctgttcact
     2941 gtcgtcgtct gtatcattct tcttgtatct gtggtgccag cgcacctcgg cattcagtat
     3001 taccaaatga tcagcgaaga cgactacgca acgtttacct ggatgaacga tcacctggac
     3061 gagtatcgga atgaaaccca tctctacgac agagcagcag tcaatccgtt ttatgcgtct
     3121 cctttttcgg ctgtgacagg actctacatt gtctcctctt caatgcatcc gatttacgga
     3181 tatgagttac atacacagat ggaaaccttc ctcaacgaca aatgtgttga cactggtttc
     3241 ttagatgacc ataaaattag tgtcatatac ggggattgca ataacaacaa tctcacgatg
     3301 atttatccag atgtatacct gtatcctgga ttgaaaaaat aataatatgc aaggtttttt
     3361 tatgaaggat catacactaa gaatgacaat tgttgtcggt actcgccctg aaataatcaa
     3421 gatgtctcct ataattcgtg aatgtaaaag aagactagat tattttatct tgcatatgag
     3481 tcagcattat tcctataata tggatcaggt tttttttgaa cagcttgagc tgacgatgcc
     3541 aaaatacaat ctttatgtcg gctctggtac gcaaacagaa caatacggaa gagctcttat
     3601 cggaattgag caaatgttac tcaaagaaca gcctgctgtt gtattggttg gaggtgatat
     3661 aaatctccat agataaaata tctgtcgtca ttcccgttaa aaatgaagaa cataaaatta
     3721 gtaaatgtct ggaggccgtc ataaaccaaa cattatctcc gtatgaaatc attgtggtgg
     3781 atggtcattc aattgacgat actgttaaaa aagcacagag tttttcagta aaaatattgt
     3841 acgaagatta tagaacaagg gccggtgcct gccatgttgg actgaaaaat gcaacaggcg
     3901 aatacatcgc ttttactgat gcagattgta ttccaagaga aaattggtta gaagctgaat
     3961 taaaggtgat aaaaaaacat agaaatgttg caggtgttgg ttgttgtatc gacaatatca
     4021 gtaaaggagt ttgggaagaa actattaacg aaattagcaa aacttttatt ggcagtggac
     4081 gatctattca gggacgacaa tttacgaaag aaaagaaagt tctcagtata agtggttgtc
     4141 atgctctcta cagaaagaaa atgcttgagg acgtcggtgg attcaacgta cgacttaaaa
     4201 cttgtgaaga cacagaaatc aacaaaagat taagaaaaaa gggttatgat ttgatataca
     4261 gtcctaaaac cagtgttctt catgatcata cccgcggaac aaaacttttt gcaaaaagga
     4321 tgtttcaata tggatggggt agagcacaaa gtaaattatt tgatatgcaa ttattg
//
LOCUS       DATMXJ010000123        10171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33813, whole genome shotgun sequence.
ACCESSION   DATMXJ010000123 DATMXJ010000000
VERSION     DATMXJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33813"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VN377_03355"
     CDS             complement(<1..812)
                     /locus_tag="VN377_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR27457.1"
                     /translation="MKTMVYRRDIAWKAFVVSVTFCLLFLPGYALAQTPNIVNDPTPH
                     KPAYLQGKSDLVDVQVSQRFAPLHPVPPTTMNLNITELLQQVDESLILGYLENLTSFG
                     PRLTGTPACDQSAHYIYETFQDMGLEVRYHNYTDDTVSGSNVEATLNGTDSTNVFIIC
                     GHYDCVAAGPGADDDGSGVAAVLAAAEIMKNYEFYHTIRFVAFSGEEQGLIGSYHYVE
                     DAYNNNDTIIAVLNADMIGFAPLPSDGTKGKIFENSASEWIVSFTQDISQVYS"
     gene            complement(940..2463)
                     /locus_tag="VN377_03360"
     CDS             complement(940..2463)
                     /locus_tag="VN377_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27458.1"
                     /translation="MKELEKTSSVCPACFQEGKIHKIDAKIIEDDGKVWIAKTCDEHG
                     DFKDIYFGDIALYERWMKFGKRGDAIPDVKTSLFDDPMLYPAHRSQSVLTNLLVTNRC
                     NLRCGYCFMNAGASGYVYEPSLDEIKKMLLQARNERPMGSKAIQITGGEPTIREDLFE
                     IIRMTREAGFSHIQLNSNGIKLAESVEYCKKLKEAKVNTVYMSFDGVTKETNPWIDQL
                     IQTIENFRQANLRAVLVPVLIGGKNLHETGKIIQFALDHIDIVRGVNFQPVSFCGRIT
                     KIKDEKRETQRVDYVRMMEVIEKEFNGQITRDDFYPVPFVYPISELIESLKGEKQVEF
                     TAHPGCGGATYIFLEDGKPLPITRFIDVEGFMAFINEQAAVKGPLKKVRVAASFLKNV
                     DKFVDKEKAPKDFDLKKILIDAAVGGSYDSLRGFHYKSLFVGSMWFMDTFNLNLHRLE
                     RCCIHYTTEEGIIPFCSYNGLGYGDKIREKHSVPIKEWEKKTERPMRDDLWKDGPLT"
     gene            2750..3397
                     /gene="rpe"
                     /locus_tag="VN377_03365"
     CDS             2750..3397
                     /gene="rpe"
                     /locus_tag="VN377_03365"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWR27459.1"
                     /translation="MVIVAPSILSADFTKLLEEITAVTEGGAEWLHIDVMDGHFVPNI
                     TIGPVVVENIRKKTSLFLDCHLMIEHPEKYVDAFLKAGADLITIHRESVSDPLVVIRH
                     IKESGGKAGISINPETSFESIRSLIPEVDLVLIMSVHPGFSGQHFIPEVIPKLKEASE
                     AIKKTKKRIYLQIDGGINNQNAGLVKQNGADVLVAASFIFHSKDYHTAIKQLKEA"
     gene            complement(3402..4076)
                     /gene="tpiA"
                     /locus_tag="VN377_03370"
     CDS             complement(3402..4076)
                     /gene="tpiA"
                     /locus_tag="VN377_03370"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWR27460.1"
                     /translation="MLKTPAIVLNVKTYTESTGVNALELARLMEKISKETNVSMAIAV
                     QACDITRCADEVSIPVYAQHIDPIKPGSSTGWTLPEAVKAAGAKGTLINHSEHRLILA
                     DIDTCLMRAKDLGLDTLVCTNNVATSKAVSTFFPSMIAVEPPELIGGDISVTSADPGI
                     VSNTVQAVRSINKTVKILCGAGVKNGRDVAKAIELGADGVLLASGVVKAKNKEEVLRD
                     LAAGIH"
     gene            complement(4171..5280)
                     /locus_tag="VN377_03375"
     CDS             complement(4171..5280)
                     /locus_tag="VN377_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWR27461.1"
                     /translation="MDTSIEKQIKEIEDEIFKTQKNKATEHHIGKLKAKIARLREIGE
                     KRKSAGAKGKGFSIKKTGDVTVGIVGFPSVGKSTLLNQLTAADSKVGDYDFTTLEAIP
                     GMMKYKGAEIQILDLPGLIVGASKGRGRGREVISAVRSVDLLLLMIDAFNLHQLNSIE
                     QELHIAGVRLNQKRPDVVISKKTYGGIIVNSTVPLTNLNELLVKSIASEFVINADVTL
                     REDLTEDQLIDAFAQNRVYISALVVINKTDLVPSDLLKNIIIGLHQRRWDVCAISAKN
                     KENLDHLREQIFLHLRLIRIYLKPVGKKPDYVQPMILREGQTIADACRYIHRDFHRNF
                     RYASVWGPSAKHSGQKVGLEHVLKDQDVLTIVVTK"
     gene            complement(5341..6279)
                     /gene="arcC"
                     /locus_tag="VN377_03380"
     CDS             complement(5341..6279)
                     /gene="arcC"
                     /locus_tag="VN377_03380"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HWR27462.1"
                     /translation="MKKRVVIALGGNALIKKGQKGTIYEQFANTRGSCRSIVQMIKEE
                     WDVVITHGNGPQVGALVLQNQAARDITPEMPLGICVAESEGLIGYMIQQCLSNALKRE
                     NIEKPVIALITQVIVDPMDPSLENPTKPIGPYYSEDESIAMLKQGYHMTRLPRGWRIV
                     VPSPDPLRIVEGDIIRKMLDDDIIIIASGGGGMPVIEKEGWGLDGLEAVVDKDLAAER
                     LAEAVGAKVLLILTDVEKVYLHYNTPSQKPIDKVTYKEMKQYYLNSEFPQGNMGPKIL
                     AALRFLEFGGERVIISNIEQGWEALQGQTGTLIVRK"
     gene            6367..7005
                     /locus_tag="VN377_03385"
     CDS             6367..7005
                     /locus_tag="VN377_03385"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HWR27463.1"
                     /translation="MFEQERERLVVHLCKEGYIKTDSVKQAFLTVPREEFIPDFLKNY
                     SYVDTPLDIGNGQTISAPHMVAIMCEALDIEKGQKILEIGTGSGYHAAIVAQLVGETG
                     MVYTIERFESLATKAKQNLQNINCSNVIVEVGDGSCGLSRYQPYDRIYVTCAAPSIPQ
                     PLIDQLRDPGKLLVPVGDMYCELTLLEKEKQKQTIHHLGGCVFVPLVGRYGH"
     gene            complement(7083..7586)
                     /locus_tag="VN377_03390"
     CDS             complement(7083..7586)
                     /locus_tag="VN377_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HWR27464.1"
                     /translation="MDEVKFKLVAIEKDDDINFILGQTHFIKSVEDLYEAMKNTVPNG
                     KFGIAFCEASGACKIRSEGNDTVLKKLAEKNAMTIGAGHTFLIFMKDCYPLNVLNAIK
                     NVPEVCSIFCATSNPTKVVVAESQQDDGIGRGILGVIDGFIPKGMENNEDFSWRKKLL
                     RDIGYKL"
     gene            complement(7801..8862)
                     /locus_tag="VN377_03395"
     CDS             complement(7801..8862)
                     /locus_tag="VN377_03395"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27465.1"
                     /translation="MKKKILHYMICVCVVLTTFSAIGIPYVSADPPVEPSQPIPSSGS
                     SSVTVFVDLQWTTGDVNLTYDVYFGTENPPPLLVVNQSGTIFEPDRLSLNTTYYWQIV
                     AHNNLQESTPGPIWSFTTAGDQPPFKPTVLDGPPNAGKGIPLEFVTVAPDPEGDNVSY
                     QWDWGDGNVSEWYGPYAFGEQTNATHVWADNGSYEVKVRARDDLGKQSGWSKAYDVMI
                     APQIQLSNCKPGYLYFVFLTFDKTYGYIYSLDLLGVSLIISTGGMRVDAAGSDSVRTV
                     VFEMQNKFLEDQRWNATSNNQTGSAFEGYFYLNDGLYETTASAYDSAGRLIDRATRSY
                     VIYYEWKFILLKQLLGLGT"
     gene            complement(8952..>10171)
                     /locus_tag="VN377_03400"
     CDS             complement(8952..>10171)
                     /locus_tag="VN377_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27466.1"
                     /translation="PDNGMNWSVIVSGQNNTGSYLWNTTVVPDGDQYLIRVSATDLAG
                     NVGSDTSDSVFSIENFDTVPPQVLVIYPNGGEGLVGDVAILWSASDDVTMDLNGSILI
                     EYSPDNGMNWSVIVSGQNNTGSYLWNTTVVPDGDQYLIRVSATDESNNTGSDTSDSTF
                     SLLNNYNYPPIIPQVIGPSAVGNGISFDFTANTTDPEGDQIYYLFDWGNGNISGWLGP
                     VNSSESVNASYAWRDDGNYTIRVKAKDIFESESNWSVVHPISIAEQINFSNVKLGHVY
                     FKLFSFNRSFIFSDFLARLGVVIILTSHPMELEAYATDIVQSVTFKVENQMQVETVEI
                     IDDNGSDGFSCSMNVSRGPYVLNITAYDANGLLIDHYSLFTVFFVRIGRYAMGSSGEG
                     RLLRLRSIHPLRH"
BASE COUNT         2841 a         2429 c         2069 g         2832 t
ORIGIN      
        1 gaataaacct ggctgatatc ctgggtgaag ctcacaatcc attccgaggc agaattctca
       61 aaaatcttcc ctttggtgcc atctgagggt aatggagcaa aaccaatcat gtctgcattg
      121 aggaccgcaa tgatggtgtc attgttgtta taggcatcct ccacgtaatg atagctccct
      181 atcaaaccct gctcctcccc tgaaaaagca acaaatcgga tcgtatgata aaactcgtag
      241 ttcttcatga tctccgctgc ggcaaggacc gctgcaacac cggatccgtc atcatccgcc
      301 ccaggtcctg cagcaacgca atcataatga ccacaaatga tgaagacatt cgtagaatca
      361 gttccattta acgttgcctc gacgttgctc ccggaaaccg tatcatcggt ataattatgg
      421 taccggacct ctaaacccat gtcttgaaac gtctcataga tgtaatgagc tgattgatcg
      481 caagcaggtg tccctgtgag acgtggacca aaactcgtca gattctctaa atacccaaga
      541 atgagtgact cgtcaacctg ctgcaacaat tcagtgatat taagattcat tgtggtcgga
      601 ggaacaggat gtaatggagc gaaacgctgg gagacctgga catcaacaag gtcgctttta
      661 ccttggagat acgcgggctt atgaggcgtg ggatcgttta ctatatttgg agtctgtgca
      721 agagcatacc ctggtaggaa taagagacaa aaggtcacac tcacaacaaa agccttccaa
      781 gcgatatccc ttctatatac catcgttttc atactactat cccctatttc ttcatatttt
      841 tgtacagcta tatatttgat tgcgctatat atatattttt gtaccaacta acaccgaaca
      901 aaagatgaag aacaagagag aaaaaaggag aaagaagttc taggtcaaag gaccatcttt
      961 ccacaggtca tcgcgcatgg gccgctcagt tttcttctcc cattctttaa taggaaccga
     1021 gtgtttctca cggatcttat ccccataccc aagaccatta taggagcaga acgggatgat
     1081 gccttcctcg gttgtataat ggatacaaca ccgttcgagt cgatggagat tcaggttaaa
     1141 cgtatccatg aaccacatcg agccgacaaa caggctttta taatggaaac cgcgcaagga
     1201 atcatagctt ccaccgacgg cagcatcaat caagatcttc ttaaggtcaa aatctttcgg
     1261 tgctttttcc ttatcgacaa atttatcaac gttcttgagg aacgatgcag caacccgcac
     1321 ctttttcaaa ggtcctttca ctgctgcctg ttcattgata aatgccatga atccttccac
     1381 atcgataaat ctggtaatcg gcaatggttt cccatcctct aggaaaatat aggttgcgcc
     1441 accgcaccca gggtgcgcgg tgaactccac ctgtttttcg cctttcaatg attcaattaa
     1501 ctcagagatc ggatagacaa aaggaacagg atagaaatca tcccgggtga tctgaccatt
     1561 gaactctttt tcaataacct ccatcatgcg gacataatcg acacgttgag tctcccgttt
     1621 ctcatccttt atttttgtga ttcttccaca aaacgagacc ggttggaaat tcacgcctcg
     1681 gacaatatcg atatgatcca acgcaaactg gatgatcttc ccagtctcat gaagattctt
     1741 cccaccaata agcactggga caagcaccgc tcgcagattt gcctgtcgga agttttcaat
     1801 cgtctggatc agttggtcta tccaggggtt tgtctccttg gtgactccat caaagctcat
     1861 gtacaccgtg ttgaccttcg cctctttcag tttcttacaa tactcaacac tctcagcaag
     1921 tttaatacca ttcgaattaa gctgaatatg agaaaaacca gcttctcgcg tcattcgaat
     1981 gatctcaaag aggtcctcac ggatggtcgg ttccccgcca gtgatttgga tcgctttcga
     2041 acccattggt cgttcgttac gagcctgcag taacattttt ttaatctcat ctaacgaagg
     2101 ctcatagaca taccctgatg caccagcatt catgaaacaa tatccacatc gaaggttgca
     2161 tcggtttgtc accaataaat tagtcagcac actttgagat cgatgcgcgg gatacagcat
     2221 cggatcatca aacaacgatg ttttcacatc agggattgca tccccgcgtt ttccgaattt
     2281 catccatcgt tcatataacg caatgtcacc gaagtaaatg tctttaaaat caccatgctc
     2341 atcacatgtc ttcgcaatcc agactttccc gtcgtcctca atgatctttg catcgatctt
     2401 atggattttt ccttcttgga aacatgcggg acataccgag gatgttttct ctaattcctt
     2461 catatctgtc aacgttctcc agaattcaaa aggactatat ggtcttttct ttatatattt
     2521 taactatctc tgaagctctc cgagaagaca tatgggatgg gatgggcagg ccaacagaaa
     2581 aaagatggtg ttcggcctgc accatatcgg tgttcgaaga ctgacgaggg catttcatct
     2641 ataaaagtct acgctgtcat tatatcattt tgacagaata acagagaacc tattcagaag
     2701 aatcgtttgg ttttataaag ccctgcatca taccatcaga cgagtaatca tggtgattgt
     2761 cgcgccttcc attctctccg cggatttcac aaaacttctt gaagaaatca ccgcagtgac
     2821 tgaaggggga gcggaatggc tccacattga tgtcatggat ggtcatttcg tcccaaatat
     2881 cacgatagga ccagttgtag tagaaaatat caggaaaaaa acatcgttat tccttgattg
     2941 tcatctgatg atcgaacatc ctgaaaaata tgttgacgcg ttcctgaagg caggggctga
     3001 tcttatcacg attcaccggg agagcgtatc cgaccctctt gtggtaatca gacacatcaa
     3061 ggaatctggt gggaaggcag gtatttctat caaccctgag acatcgtttg aatcaatccg
     3121 atctctgatc cccgaggtcg atttagtgtt gattatgtct gtccatcctg gtttttcagg
     3181 gcaacatttc atccctgagg taatcccaaa gcttaaggag gcaagcgagg ccatcaagaa
     3241 aacaaaaaaa cggatttatc tccagattga tggtgggatc aacaatcaga atgctgggct
     3301 tgtaaagcag aatggtgcgg acgtcctggt cgctgcgagt tttatttttc acagtaagga
     3361 ttatcatact gcgatcaaac agttaaaaga agcatagctg attagtggat accagcggct
     3421 agatctctca ggacctcttc tttatttttc gctttcacca caccactggc aagaagcacc
     3481 ccgtctgcgc ctaactcaat tgcttttgcg acatctctgc cgtttttcac cccagcgcca
     3541 cagaggattt tcacggtttt attaatgctt ctgaccgctt gtaccgtatt gcttacgatg
     3601 ccaggatccg cggaggtgac agagatatca ccaccgataa gctcaggtgg ttctaccgca
     3661 atcatcgatg ggaaaaaggt cgagaccgct ttagaggtcg ccacattatt tgtacagaca
     3721 agagtatcta acccaaggtc cttcgccctc ataaggcagg tatcgatatc cgcaagaatc
     3781 agacgatgct cgctgtgatt gatgagagta ccttttgcac cagcagcttt cacggcctcg
     3841 ggtaacgtcc aacccgtgct actcccgggt tttatcggat cgatatgttg ggcatatacg
     3901 gggatgctga cctcatcggc acaacgggtg atatcacatg cttgcacagc aatagccata
     3961 ctgacgttgg tttcttttga tattttctcc atcagtcttg ccagttccaa tgcattcaca
     4021 ccagttgatt ctgtgtaggt cttcacgttt aaaacaatcg ctggagtctt caacatatct
     4081 catcaccaat caatatatct acaggggtaa tcgagtagtg aaataaataa gctttctttc
     4141 cgtcacaaaa cgaaaatctg gtttgattca ttattttgta acgacaatcg tcaggacatc
     4201 ctgatccttt aacacatgtt caaggccgac cttctgacct gagtgtttcg cagaaggacc
     4261 ccacaccgag gcgtaccgga aattcctgtg aaaatcacga tggatatacc gacacgcatc
     4321 agcaatggtt tgaccctctc gcaatatcat aggttgtaca taatcaggtt tctttcccac
     4381 cggtttcaag tagatccgaa taagtcgaag atgtaaaaaa atctgttctc tcaaatgatc
     4441 aaggttctcc ttgtttttcg cagagatcgc gcacacgtcc catcgtctct gatgtaatcc
     4501 aatgataata ttttttaaca gatcagacgg gacgagatcg gttttattga tcaccaccaa
     4561 agcagagatg tacacacggt tttgcgcaaa cgcatcaatg agttgatcct cggtaagatc
     4621 ctctcgtagc gtgacgtctg cattgatcac aaactcagaa gcgatggatt tgacaagtaa
     4681 ctcattgaga tttgtcaacg ggaccgtgga attgacaata attcctccgt atgttttttt
     4741 actgatgacg acatcgggtc tcttctgatt cagccgcact ccagctatgt ggagttcctg
     4801 ttcaattgag ttcagctgat gaagattaaa cgcatcgatc attaacagga ggagatctac
     4861 gcttcttacc gcagagatga cctctctgcc tcgtccacgt ccctttgatg caccaacgat
     4921 gagaccaggc aaatcaagga tctggatctc agctccttta tacttcatca tccctgggat
     4981 cgcctccagg gtcgtgaagt cataatcacc aaccttacta tcagctgctg tcagttgatt
     5041 gagaagcgta cttttcccca cactaggaaa ccccacgata ccaacagtca catcaccggt
     5101 ttttttaatc gagaagcctt ttcctttcgc accagcgctt tttcgtttct caccaatctc
     5161 tcgcaaccgt gcgatttttg ctttgagttt gccgatatga tgttctgtcg ctttattttt
     5221 ctgagttttg aaaatctcat cttcgatttc tttgatctgt ttttctattg aggtatccat
     5281 agctgtacct gctctatatg cagaggatgt ttataaaaaa cacgtgtaca aaaacggaag
     5341 ctatttgcgt acaatcagag tgcctgtctg gccctggagc gcttcccatc cttgttcgat
     5401 atttgagatg atgacacgtt ccccaccgaa ttctaagaaa cgcagggcag ctaggatctt
     5461 tggccccata tttccttgcg ggaactcact attcaggtag tattgcttca tctccttata
     5521 ggtgaccttg tctataggct tttgcgaagg ggtgttataa tggagataga ctttttccac
     5581 atcagtaaga atcaataaga cctttgcgcc gacagcctca gcgagtcgtt ctgctgctaa
     5641 atccttatcc accacagctt ctaatccatc aagaccccag ccttcttttt cgataacagg
     5701 catgcctccg ccgcctgatg ctatgatgat gatatcatca tctaacatct tccggatgat
     5761 atccccttcc acgatgcgca acgggtctgg tgaggggacg acaatcctcc atccgcgagg
     5821 caaacgggtc atgtgatacc cttgttttaa catggctata gattcatctt ctgaatagta
     5881 cggtccgatt ggcttggtcg gattctcaag agagggatcc attggatcca caatcacttg
     5941 ggtaataagt gcaatcacgg gtttctcgat gttttctcgt tttaatgcat ttgagaggca
     6001 ttgttggatc atatatccga tgagtccttc gctttctgca acacagatcc ctagcggcat
     6061 ctcaggggtg atatcccttg ccgcctggtt ctgaagaaca agcgctccca cctgaggacc
     6121 gttcccatgg gtgatcacca catcccattc ttccttaatc atttggacaa tggatcgaca
     6181 gcttcccctg gtgttggcaa actgttcgta aatggtccct ttctgacctt tttttattaa
     6241 tgcattacca ccaagtgcaa taaccactcg ttttttcata acagagggct ggaaatagat
     6301 tcttggtata aaaatcagat ggtgcaagga gatatatatc ccatacaatt tctccaatag
     6361 aaacatatgt ttgaacaaga acgggaacga ttggttgtac acctatgcaa agaaggctac
     6421 atcaaaaccg attcggtaaa acaagcgttt ctgacagtcc ctcgagaaga gttcataccg
     6481 gatttcctga aaaactattc ctacgttgat acacctcttg atatcgggaa tggtcaaacc
     6541 atctccgcac cacatatggt agcgatcatg tgtgaggcat tggatataga aaaaggtcag
     6601 aaaatcttgg agattgggac tggatctggg tatcatgctg cgattgtggc tcagcttgtc
     6661 ggagaaacag gaatggtgta caccatcgaa cgatttgaat cacttgcaac aaaagcaaaa
     6721 caaaaccttc agaacatcaa ttgttcgaac gtgatcgtgg aagtaggtga tggttcttgt
     6781 gggctttccc gctatcaacc ttatgatcgt atttatgtca cctgtgccgc cccatctatt
     6841 cctcagccgt tgattgatca attgcgcgat cctggaaaat tactcgtccc agtgggagac
     6901 atgtactgtg aattaacgct tcttgagaaa gaaaaacaaa aacagacaat ccaccatctt
     6961 ggaggatgcg tgtttgtgcc acttgtcgga aggtacggac attaaatcag agtgtagaga
     7021 aaggaaatgg tacgtttact tctccgataa atgatgtttc atttggtgtt tctgctgttg
     7081 gttcagagct tataaccgat atcccgtaac agtttctttc tccaggaaaa atcctcatta
     7141 ttttccatcc ctttcgggat gaatccatcg atgacaccaa ggatgcctcg gccgattcca
     7201 tcgtcttgtt gtgattctgc cacaacgacc ttcgtgggat ttgaagtcgc acagaaaatc
     7261 gaacacacct ccgggacatt cttaatagca ttgagcacat tgagtggata acagtccttc
     7321 ataaagataa gaaatgtgtg acccgcaccg atagtcatcg cgtttttctc cgccagtttc
     7381 ttaagaaccg tatcgttgcc ttcgcttcga attttacacg caccactcgc ttcgcaaaag
     7441 gcgataccga attttccgtt agggaccgtg tttttcatcg cctcgtaaag atcctcaacc
     7501 gatttgatga aatgagtttg cccaaggatg aaatttatat catcatcttt ttctattgct
     7561 accagcttga attttacctc atccatataa caactcccct ttgaataaaa aaaagaatag
     7621 aacaagagta tttaaggata taatgaaata agatcccacc cactcaattc tccttgaaat
     7681 caaaggaatc gctcatcgaa tgaaaacgag ggaaccaatc aaaggtctta tacgtatgat
     7741 catcctcaag gttgcttcca tttgtaggtg ttctgaattg agggtttgga ttcgaagtct
     7801 ttaggtacca aggccaagta actgcttcaa caagatgaac ttccattcat aatagatgac
     7861 gtaacttctt gtcgcccgat cgatcaaacg accagcacta tcataggcgg acgctgttgt
     7921 ctcatacaga ccgtcattta aataaaagta gccttcgaaa gcactcccgg tctggttatt
     7981 agaggtcgca ttccatcgtt ggtcttctaa aaatttattc tgcatttcaa agacaacggt
     8041 acgaactgaa tctgaacctg ctgcatccac tctcatgcca cctgtgctaa tgatgagtga
     8101 gaccccaagg aggtcaagtg aatagatata cccataggtt ttgtcaaatg tcaagaagac
     8161 aaagtaaaga tatcctggtt tacagtttga caactgaatc tgcggtgcga tcatcacatc
     8221 ataggcttta gaccaaccac tttgtttccc caaatcgtcc cgcgctcgta ccttcacctc
     8281 gtagcttcca ttatcggccc atacatgggt tgcgtttgtc tgttcaccaa acgcataagg
     8341 accataccac tcactaacgt tcccatcacc ccaatcccat tgataggaca cattatcacc
     8401 ctccgggtca ggagcaacgg tcacgaattc cagtggaatg cctttccctg cgttcggagg
     8461 accatcaaga accgtgggtt tgaacggtgg ttgatcccct gcggtggtga aactccaaat
     8521 cggacctgga gttgattctt gcaggttatt atgtgcaaca atctgccaat aatacgtcgt
     8581 gttcaatgaa agacgatccg gctcaaaaat cgttcctgac tgattcacca cgagaagcgg
     8641 cggtgggttt tcagtcccga aatagacatc ataggtgaga ttgacatcac cagtggtcca
     8701 ttgcaaatca acaaaaacag taacactgct cgacccggac gaagggatcg gttggctcgg
     8761 ctcaacgggt ggatctgcag aaacataggg gataccaata gctgaaaaag tagtcagcac
     8821 cacgcataca catatcatat agtgtagaat ttttttcttc atataatccc tcttattttt
     8881 ttacttcacc attgagtatt tttcttttat ctattttaac tacgatgtat ttctatttaa
     8941 acattgtgga ctcagtgacg caacggatgg atgcttcgga gacgcaacag acgaccctcg
     9001 cctgaagatc ccatggcata tctcccaatg cgaacaaaga aaacagtgaa gaggctgtaa
     9061 tgatcaatta acagtccatt tgcatcatag gcagtaatgt taagtacata tggaccacga
     9121 gaaacattca tggaacagga gaaaccatca gaaccattat catcgattat ttccacggtt
     9181 tcaacttgca tttgattctc cactttgaat gtaaccgatt gaacaatatc tgttgcatat
     9241 gcctcgagtt ccattggatg gctcgttaat atgatgacga cacccagacg tgccaggaaa
     9301 tcagagaaaa taaatgatct gttaaaggaa aaaagtttga agtagacatg tccgagtttg
     9361 acattagaga agttaatttg ttccgcgatt gaaattggat ggacgacaga ccagttactt
     9421 tcactttcga agatatcctt tgctttcact cggatggtat aattcccatc atctctccag
     9481 gcataggatg cattgacaga ttcgctagag ttcactggac caagccagcc gctgatattc
     9541 ccattgcccc agtcgaacaa gtagtagatt tggtctcctt caggatcggt cgtattggca
     9601 gtaaaatcga agctgatccc gtttccaact gcagaaggcc caattacttg tgggattata
     9661 ggtggatagt tataattgtt caggagagaa aacgtactat cagatgtatc tgacccagtg
     9721 ttattggact catccgtggc actcactcgt atgagatact gatcaccatc agggacaacc
     9781 gtcgtattcc acaaataaga accagtattg ttctgcccag aaacaatcac actccaattc
     9841 atcccattat ccggactata ctcaatcaga atactcccat tcaaatccat cgtcacatca
     9901 tcagaagcag accagagaat agcaacatcc ccaacaagcc cttcgccacc attcggataa
     9961 atgaccagca cttgcggtgg aacagtatcg aaattttcaa tagaaaacac actatctgac
    10021 gtatcggaac caacattccc cgccaaatcc gtcgcactca ctcgaatcag atactgatca
    10081 ccatcaggga caaccgtcgt attccacaaa taagaaccag tattgttctg cccagaaaca
    10141 atcacactcc aattcatccc attatccgga c
//
LOCUS       DATMXJ010000124         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33835, whole genome shotgun sequence.
ACCESSION   DATMXJ010000124 DATMXJ010000000
VERSION     DATMXJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33835"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VN377_03405"
     CDS             complement(<1..1050)
                     /locus_tag="VN377_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWR27467.1"
                     /translation="MTESTMQKEYDLQFFHTNGYYRKKCKACGDYFWTLDAQAEYCGD
                     QPCVKFSFIGNSIAKKPLTLAEVREGFLSYFEQHGHSRLQYPETGPRCPVVARWRSDI
                     YLTIASIADFQPHVTSGVVPPPANPLVISQPCIRLNDLDEVGKSGRHLTTFEMMGHHA
                     FNKNIDEIYWKEETVAYCDEFFRKKIGIPREAINYKEQLWIGGGNAGPCVEVLAGGLE
                     IATLVFMNMKEDGHGKTIIEGKPYAPNPLNIVDTGYGLERIAWFTSGTPTVYETAFPK
                     MLSYLLDSLKDTSDMGSIYALADHTKCLSFMLGDGIVPSNVKAGYLARLLIRRSLRFI
                     EKLQLQYSLRHLVELQ"
     gene            1193..2425
                     /locus_tag="VN377_03410"
     CDS             1193..2425
                     /locus_tag="VN377_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWR27468.1"
                     /translation="MKKIIPCLLVVVLVSIGSVGITSAFNEHKTDKKTQLQTTSAGYE
                     IQWMMEYGNNPGSDARYQGPQPIGDSDNDGKNELLISGRDCTIRVMKWNENQQTYSQV
                     RAIRPPLYPFIHCDAGGMAIGDVTNDGKNEVAATWYTSVYRYFVFRYWLVGLNPYIFN
                     HNGGSADCLIGDYDNDGKNELILSGGSGWQENSSVAEITVFRWTGLFLKKIAEWKDPD
                     VNGYVYMAGLGDVDEDGENEIVCAYENKVMVLNWDAQNAVFVPMQIYRTYGHVSPFGI
                     VCKDCDNDGNAEILLSYYDPRITIFKWNGTGYPIQFDITWSGDDPVIEGIDVGDTDDD
                     GLNEVCAGAGMIHILQWNGSTYVEEAVLPTFGWMAVVCIGDCDNDGKNEINAGNVDVN
                     IDAGELFTECVFKYNPET"
     gene            2529..3230
                     /locus_tag="VN377_03415"
     CDS             2529..3230
                     /locus_tag="VN377_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR27469.1"
                     /translation="MGVKEVLAEKIAGEITLSPKPGETLRKWRTTFNIAQAELANHLG
                     LAQSVISDYESGRRKSPGIHSVKKIVHAFLAIDEKKHGGTIIQQYQFMVKTQDGIIDI
                     MEYPYAIPMETLIKEIEGRVVTSPSVSIDRSVKGFTLIDGIETIKTLTATNYPNLYGW
                     STERALIFTKIQFGRSPMIAIRIHPVKPSLVVYHQPGGVDPLAVQLAERENMPLVITE
                     MPLEELRKKLILLGK"
BASE COUNT          904 a          668 c          820 g          944 t
ORIGIN      
        1 ttgcagttcg acgaggtgtc gtagactata ctgcagctgt aatttttcta tgaatcgaag
       61 tgatcttctg atgagtaatc gtgcgaggta tcctgccttg acatttgagg gaacgattcc
      121 atcaccgagc ataaaggaga gacatttggt gtggtctgca agtgcgtaaa tgctgcccat
      181 gtctgatgta tcttttagtg aatcaagtag gtatgatagc atttttggaa acgcggtttc
      241 atacactgtt ggtgtaccgg aggtgaacca ggctattcgt tccaatccgt atcccgtatc
      301 aacgatattt aaaggatttg gtgcgtaggg ttttccttcg atgattgttt ttccgtgtcc
      361 atcctctttc atgttcatga acacaagggt tgctatctcc aatccacctg cgaggacctc
      421 aacgcagggt cctgcgtttc ctccaccgat ccagagttgc tctttgtaat ttatcgcttc
      481 acgtggtatg ccgattttct ttctgaaaaa ctcatcgcag tatgcgactg tttcttcttt
      541 ccagtagatt tcgtcgatat ttttattaaa tgcatggtgc cccatcatct cgaaggtggt
      601 gagatgtcga ccgctttttc ctacttcatc aagatcattg agccgtatgc atggttgtga
      661 gatgaccaac gggtttgccg gtggagggac tacccctgag gtcacatggg gttgaaaatc
      721 tgctatggaa gcaatggtaa ggtaaatatc ggaccgccag cgggcgacga ctgggcatcg
      781 tggtcctgtt tccgggtatt ggagtcgtga atgaccatgt tgttcgaaat atgagaggaa
      841 tccctcacgt acctctgcaa gagtcaatgg tttttttgca attgaatttc caatgaagct
      901 aaattttacg catggttgat ctccgcagta ttctgcctgc gcatcgagtg tccagaaata
      961 gtccccgcat gctttgcatt tctttctgta atatccgttt gtatggaaaa attgtaagtc
     1021 gtattccttt tgcatggttg attccgtcat aggtcttaac tggtctggaa tagacaaaag
     1081 gtttaaagtt ttatctttca acaacccaaa tcgttatctc atatgaacat ctttttttat
     1141 gaataacgat atatttataa ggagatatac ccttgaatat agtaggggta atatgaagaa
     1201 gataattcct tgtttattag tggttgtgct agtatcaata ggcagtgttg ggatcaccag
     1261 tgctttcaac gaacataaaa cagataaaaa aacacaactc cagacaacga gtgctgggta
     1321 tgaaatccag tggatgatgg agtatgggaa taatcctggt tcggatgcac gttatcaagg
     1381 accacaaccg attggagata gtgacaatga cggtaagaat gaactactca tcagcggaag
     1441 ggattgtacc atccgtgtta tgaaatggaa tgagaatcaa caaacctatt cacaggttcg
     1501 tgccattcgt cccccattgt atccatttat ccattgtgat gctggtggga tggcaattgg
     1561 ggatgtgaca aacgatggga aaaatgaggt ggcggcaacc tggtacacct cagtttatcg
     1621 ttatttcgta tttagatatt ggttggttgg attaaatcct tatattttta atcataatgg
     1681 aggcagtgcg gattgtctca ttggagatta tgataatgac gggaaaaatg agttgatttt
     1741 gagtggtggg tctggatggc aagaaaatag ctcggtcgct gagatcacgg tgttccgatg
     1801 gactgggctc ttccttaaaa aaatagcaga atggaaggac ccagatgtca atggttatgt
     1861 ctatatggcg ggacttggtg atgtcgatga ggatggagaa aatgagatcg tctgtgctta
     1921 tgaaaataag gtcatggtgt taaattggga tgctcaaaac gcggtgttcg ttcctatgca
     1981 gatctataga acatatggac atgtctctcc ctttggcatc gtgtgcaagg attgcgataa
     2041 tgatgggaac gcggagattc tcctgagtta ttacgatcct cgtattacga tctttaaatg
     2101 gaacgggact ggatatccga tacagtttga tatcacctgg tctggtgatg atccagtcat
     2161 tgaaggaatc gatgtgggtg acaccgatga tgatggcctt aacgaggtgt gtgcgggagc
     2221 gggtatgata catattttac aatggaacgg gtcgacatac gttgaagaag ctgtgctgcc
     2281 tacgtttggg tggatggcgg tcgtctgcat cggggattgt gacaatgacg ggaaaaacga
     2341 aatcaacgca ggcaacgtcg atgtcaatat cgatgccggt gagcttttta ccgaatgtgt
     2401 ttttaaatac aatcctgaga catgattttc cctctttttt tcttcttttt cataattttt
     2461 tcttagggga aacgtattaa tagattgtcg atatatatat cgacagtcat tgtaggctct
     2521 gggaaaatat gggagtcaaa gaagtattag ctgaaaaaat cgcaggggaa atcaccctca
     2581 gcccaaaacc aggggagacc ctgcgaaaat ggcggaccac ctttaatatt gcacaagcag
     2641 agcttgccaa tcatctaggg cttgcccaaa gcgtcatcag cgactatgaa tcaggaagac
     2701 gaaaatcacc tgggatccac tccgtgaaaa aaatcgttca cgcttttctc gccatcgacg
     2761 aaaagaaaca tggcggcacc atcatccagc aatatcagtt catggtcaag acacaagacg
     2821 gcatcatcga catcatggaa tatccctatg caatccccat ggaaacactc attaaagaaa
     2881 tcgagggaag ggtcgttacc tccccaagcg tcagcattga tcgatccgtc aaaggattca
     2941 ccttaattga cggcattgag accatcaaaa cattgaccgc caccaactat ccgaatctct
     3001 atgggtggag cacggaacgg gcactgattt ttaccaaaat acagttcggg agaagcccga
     3061 tgatcgcgat ccgaattcac cccgtcaaac cctccctagt ggtgtaccat caacctggcg
     3121 gcgtcgatcc cctcgcggtg cagctcgcag aacgagaaaa catgccattg gtgatcactg
     3181 aaatgcccct cgaagagcta cggaaaaaat tgattttact ggggaagtga cgattgatga
     3241 ctgatgtggt cggactctgt gacatctgcg gaagacctgg aaccatgcat acctgtcagc
     3301 tttgcggccg gcttgtctgt gaacgatgct atgagc
//
LOCUS       DATMXJ010000125         7286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_39350, whole genome shotgun sequence.
ACCESSION   DATMXJ010000125 DATMXJ010000000
VERSION     DATMXJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39350"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..5532
                     /locus_tag="VN377_03420"
     CDS             142..5532
                     /locus_tag="VN377_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWR27470.1"
                     /translation="MYARPIITVNNSIGTFLMDHYTTTTLTGWVNQTYLEETSEKPGA
                     AGSNIYGNITIERSQNDSVAILSNRYDGMWGQTSGDSAVSGAILLGIIKYFNDHHSIK
                     PKYNLTFLFTTSEEFGFHGARFHVDNHSDQNIVNWIILEQSGFDQSDTGLSLCFSNES
                     QKDIYETILNDSNYNEYDSWVSNSAGAGSEQGTVNKIFKNNVCLGKGDKRIHDNGKDY
                     FVGTAWDNWHRAGADYFKGDSKDNIDRDDVNATAKLTLGLVKYFMINPDSWFDGSATY
                     TAEDSPYDTDDDNDSINVTLSVKSSLPQDKIRVNATVKSWPSNTTVFWKNYDFNVTSG
                     GTQKTLIVTLPPYLTGATSGNYSLTLNLLNSTGRINDIALHTRVYDDTDTQSGYVYLT
                     PRNNSIPNKPSDITFTEPINYTTSTTDSNLDQLQYEWVWHALLYDLHLSGDIDYAGPC
                     DSGEDCTVQHTYLLWGLKDIKVRARDDYGYLFDGSPLGWNRYGNWSEWSDILSINEQM
                     LTDFSMSCTSFSSVLNARSATELPSILLINSTYNALVFGESESLTYRWTFNGRDAVQG
                     KTTEHNFSDTGNYRVTLNVSDDNGYSTEFSVNVSVINLSANFNLSIPSPFAEINENIT
                     FTDTSAVVDEYQITDWMWDFGDGSISNNRNTTHSFTEMGLYNVSLTVWDNQSNSDTFY
                     RNIFVFLDEVPPEIQQVNDETNVKNRWLEVMIAGSFNDTESGIDHALVNITYPNGTHA
                     NYTMNHFVNGTYWYVFNDTAQTGQYNYTIWVTDFENNTSNATGYSITIPTPPILQYTT
                     TTPADNADSKDDWVNVNVTVFDPLNTSAFIDWNHSLTGYWPMESYNDTGVYDNSTYEN
                     FGAFQNGMNTRNIVPGKYGDGLEFDGSDDYLDLGTSNSLDLGTGDFTFMVWEKSHTTL
                     YSKKAMILTNSPAMESWKGYGFGVTNSPYLIVSQSAGNNITLQGTTDVTDNTWHHIAY
                     VCRSEEYLIYVDGELDADNGGWMPAKNITNAQHTMIAYDGHCSSWCYFEGLLDEPQLY
                     NRALGWEEINASYNNGLYRLSHNFTGLTDGTYSYSAHAIDTTGNQGNTETRQILIDTT
                     PPTFTNVNASPHTVGFGYNVTITADVIDNVSGVDLVTIQIIPPSGVGNSSNNTMTLIS
                     NNTYQYVFTDTWSTGQYNYTIWAIDVANNTASSSGHHFHVSAEATISIATLRNTYTGN
                     QYINITDPPNPPENYILVDRGLTWNTYYNATSGENILETFQGPVNFQEENGTWTPINN
                     TLMQLASNHPAYVYGYRQGNDRGSFGVYFKSNAQQDWPVVFTYNKSDDPTIHAVKSKL
                     VGVGYVDPQSNWAYQYLQNVQNSQGQTNCYSITYPGVFTGTDVTWSYGNTGLKEEITM
                     SNATKTVLQNHPPSQYGLNDASSYLVFITKLDYQNLNLYNNSGLLDGNVTISDAGVDF
                     KDVLGQFKCALPLGEAYELSNESARQKLTYRIIHLNGNTYLLSGLKVSDLNAMTFPVV
                     IDPTLTVYSISSDGHVYNSGTSYTTVQAASTGTVSGSGAYITIGQRKLYFPDTYYIYR
                     GFVFFNTSTLPSNAYLDDAALSLYKKDDYSSTDFDITIQNGQPTYPHNPMQSSDYNKN
                     YYSGNGGSLNTSKFTSGYNAITLSNLNWINKTGITKLCLRSSRDISGTAPTGSEYVNV
                     HSSEFLGMCPPKLVINYRNQSKIKNTGSTNIKGYLLIQIQFYNTSQAKWVIDNDTVNE
                     SIPRVINAGGSGSGSQLGLDTIFNRLVRASDLTHGTGTYRVYTAFRDPEGNNLKTNDD
                     VDLEAWWQFSKT"
     gene            5797..6243
                     /locus_tag="VN377_03425"
     CDS             5797..6243
                     /locus_tag="VN377_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27471.1"
                     /translation="MNTLDTKIKELVYFLNTLPTVKTIGSCQGHDDGGETGNFDVPYI
                     SFACIDRRVLGFLASIRFAYEDPSDILPKFRKNQPRLQADWSIHVITGEDPEYSPEEL
                     KADNRQEDYVFYVLQPDRYSYDKPSDIYGDFSEILKYYQWKWKRMK"
     gene            complement(6702..>7286)
                     /locus_tag="VN377_03430"
     CDS             complement(6702..>7286)
                     /locus_tag="VN377_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27472.1"
                     /translation="ANVYQIPPNEPKVADQIKKDFSKFNFFIVELGLNVMLARGITVP
                     KLRLNVDLKCNTNDRTDVTAVDVAPKDQFKDIKIISGKIYLGVNELLNFVPSTSIKLL
                     SKIIKIDLDPIPFSWTIKKCMINTSGPKNYKVSWLIYETDTVESFNPMLILKARKNVR
                     KITAKAKGIYLQQTGLITKHNETLPSKTIRIWPT"
BASE COUNT         2290 a         1568 c         1427 g         2001 t
ORIGIN      
        1 gtattaaagg cacattttta taaattttta gatttcatac ataatcctct tcaaaaagat
       61 ttcaaaggta ttattctcta tgatgcaaac gatgttcatt ggacagtgtg ttcatttgct
      121 caaacaaaaa atctattcca aatgtatgca agaccaatta ttacagtcaa taattccatt
      181 ggtacttttc ttatggacca ttacacaaca acgacattga ctgggtgggt taatcaaact
      241 tacctcgaag agacgtccga aaaaccaggt gctgctggaa gtaacatcta cggtaatatt
      301 acaatagaaa gaagccaaaa cgattccgtt gcaatcctct cgaacagata cgacggtatg
      361 tggggccaga cttcaggcga ctcagctgtt agcggcgcta tcctccttgg aattatcaaa
      421 tactttaatg atcatcactc tattaaacca aagtacaacc tcaccttctt atttacgacc
      481 tcagaagaat ttggattcca cggagctaga ttccatgttg acaaccattc cgaccaaaat
      541 atcgtcaact ggattattct agaacaatca ggatttgatc aatcagacac aggattatca
      601 ctctgtttca gtaatgaatc tcaaaaagat atatacgaaa ctatcttaaa cgatagcaat
      661 tacaatgaat acgattcttg ggtgagcaat agtgcaggag caggttccga acaaggtact
      721 gttaataaaa tatttaaaaa caacgtttgt ctcggcaaag gcgacaaacg tattcatgat
      781 aatgggaaag attattttgt aggaactgca tgggacaact ggcatagagc aggggctgat
      841 tattttaagg gtgacagcaa ggataatatc gatcgagatg atgttaacgc caccgcaaaa
      901 ttaacattag gtctggtaaa atatttcatg attaaccctg acagctggtt cgacgggtca
      961 gccacctata ccgcagagga ttctccatac gataccgatg atgataatga ctccattaat
     1021 gtcactctat cagttaaatc gtcgctccca caagacaaaa ttagagtgaa tgcgacagtg
     1081 aaaagctggc cttcaaatac cacagttttc tggaagaact atgattttaa tgtgacctct
     1141 ggaggaacgc aaaaaacgct tatcgtcacc ctgccaccat atttaacagg agctacaagc
     1201 ggtaattatt ccctaacact taatctgtta aattcaacag gtagaatcaa cgatattgcc
     1261 cttcatacaa gggtatatga cgacactgat acccaatcag ggtatgtgta tctcaccccg
     1321 cgaaataaca gtatcccaaa caaacccagt gatataactt tcactgaacc tattaactat
     1381 accactagta cgactgactc caaccttgat cagttgcaat atgaatgggt atggcatgct
     1441 ctcctttacg acctgcatct ctcgggggat atcgattatg ctggcccctg tgactcaggg
     1501 gaagactgta cagtacaaca tacatatctt ttatggggat taaaagatat aaaggttcga
     1561 gcgcgggatg attacggata tctgttcgac ggatctcctt tgggatggaa ccgatatggt
     1621 aattggagtg aatggtccga tattctatcg ataaatgagc aaatgctcac tgatttcagt
     1681 atgtcatgta catctttttc gtcagttctg aatgcgcgat cagcaaccga gttaccatcc
     1741 attctactaa taaattcaac gtacaatgct ctcgtatttg gcgagagtga atccttaaca
     1801 tatcgatgga catttaatgg tagagatgca gtccagggga agaccacaga acacaatttt
     1861 tcagataccg ggaattacag agtcaccctg aacgtttcag atgacaatgg gtactccact
     1921 gagttttctg tcaatgtctc cgtcatcaac ctcagtgcaa acttcaacct cagcattcca
     1981 tcaccgtttg ctgaaattaa cgaaaacatt acgtttacgg atacctccgc agtggtagac
     2041 gagtatcaga taacggattg gatgtgggat tttggtgatg gttcaatctc caataataga
     2101 aatacgacac attcgtttac agaaatggga ttatataatg tttcactaac ggtatgggat
     2161 aaccagtcga actcagatac cttctaccga aatatatttg ttttccttga tgaagtacca
     2221 cccgagattc aacaggtcaa tgatgaaaca aacgtaaaga accgatggct ggaagtcatg
     2281 atcgcagggt cattcaacga tacggaaagc ggcatcgatc acgccttggt gaatatcacc
     2341 tatcctaatg ggactcacgc gaattatacc atgaaccatt tcgtcaacgg cacctactgg
     2401 tatgttttta atgacactgc gcaaaccggt cagtacaatt acaccatctg ggtgaccgac
     2461 tttgaaaaca acacaagcaa cgccaccggt tattccatca cgatcccaac accaccgatc
     2521 ctccaatata caaccacaac accagcggat aacgcggact ccaaagatga ctgggtaaac
     2581 gtcaatgtaa ctgtttttga tccattgaac acctccgcgt tcatcgactg gaaccacagc
     2641 ctcacaggat actggcccat ggaatcctat aacgacactg gtgtgtacga taactccacc
     2701 tacgagaact tcggggcatt ccaaaacggg atgaatacca gaaacatcgt accaggaaaa
     2761 tacggggatg gactggagtt cgatggctct gatgattacc tcgatcttgg caccagtaac
     2821 agtctggatc tcggcactgg tgatttcacg ttcatggtct gggagaaaag ccacaccacc
     2881 ctatattcaa agaaagcgat gatcctcacc aacagcccag caatggaaag ctggaaaggc
     2941 tacgggttcg gcgtcacaaa cagcccctat ctcatcgtct cacaatccgc gggaaataac
     3001 atcacattac aagggacaac cgatgtcacg gataacacct ggcatcatat cgcctatgtc
     3061 tgccgttcag aggagtacct tatctatgtt gacggtgaat tggatgcaga taatggagga
     3121 tggatgcctg cgaaaaacat caccaacgca cagcatacta tgatcgccta tgacggacac
     3181 tgttcaagct ggtgttactt cgaaggtttg ctcgatgagc cgcaactcta caatcgtgcc
     3241 ttaggatggg aggagattaa cgcctcttat aacaacggac tctaccgatt atcccataat
     3301 ttcactggtc ttactgatgg aacgtacagt tattctgcgc atgcgatcga taccaccgga
     3361 aaccagggca atacagagac acgacaaata ctcattgaca ccacaccacc aacttttacg
     3421 aatgtcaatg cttcgcccca taccgttgga ttcgggtata atgtgacgat taccgcggat
     3481 gtcattgata acgtaagtgg tgttgatctt gtgaccattc agataatacc tcccagtggc
     3541 gtcgggaact caagcaacaa cacgatgacc ctgatctcga ataacaccta tcaatatgtg
     3601 tttactgata cctggtccac tggtcaatat aattacacga tttgggcgat agatgttgct
     3661 aataacacag cgagcagcag cggacatcat ttccatgttt ctgcggaggc gacgatcagc
     3721 atcgccaccc tgcgaaatac gtatactgga aatcaataca tcaatatcac cgacccgcca
     3781 aaccctccag agaactatat cctggttgac cgtgggctga cctggaatac ctactacaac
     3841 gcaacctcgg gagagaatat cctggagaca ttccagggac cggtgaactt ccaggaagag
     3901 aatggtacct ggacaccgat caacaatacc ctcatgcagc tcgcatccaa tcatcccgct
     3961 tatgtctacg gatatcgaca gggaaatgat cgtggatcat tcggtgtgta tttcaaatcc
     4021 aatgcacagc aagactggcc tgtcgttttc acctataaca aatctgatga tccaactatc
     4081 catgcggtaa aaagcaaact cgttggtgtc ggatacgtcg acccgcaaag caactgggcc
     4141 taccagtatc ttcagaatgt tcaaaacagt cagggacaaa ccaattgcta ttctatcacc
     4201 tatcctggtg tgttcaccgg tacggatgtc acctggagct atgggaacac cggacttaaa
     4261 gaagaaatca ccatgagtaa tgccacgaag actgtgttgc agaaccatcc accatcgcaa
     4321 tatggtttga atgacgcaag ctcgtatctg gtgttcatca ccaaactcga ctaccagaac
     4381 ctcaaccttt acaataattc aggattgtta gatgggaacg tcacaatttc cgatgcagga
     4441 gttgatttca aggatgtcct cgggcaattc aaatgcgctc tacccctcgg agaagcgtat
     4501 gaattgagca acgagtctgc ccgacagaaa ttaacctacc gtatcattca cctgaatggg
     4561 aatacctatc tgctctcagg tttgaaggtc tctgatttaa atgcgatgac attccccgtg
     4621 gtgattgacc caacgttaac ggtgtattca atctcaagcg atggtcatgt ttacaactca
     4681 ggcacaagct atactacagt acaggctgcg tcaactggga ccgtgagtgg ttctggggca
     4741 tatatcacga taggacaaag gaagttatat ttccctgaca cctattatat ttaccgcggg
     4801 ttcgtcttct tcaacacatc taccttaccc tcaaacgcct atctcgatga tgctgcactg
     4861 tctttataca agaaggatga ttattcatcg actgattttg atatcaccat ccagaatggg
     4921 caaccaacct acccccataa tccgatgcaa tccagcgatt acaacaaaaa ctattattcc
     4981 ggaaatggcg gctcgttgaa cacctccaag ttcaccagcg ggtataacgc tatcaccttg
     5041 agcaatctta actggatcaa taaaaccggg atcaccaaac tctgtctcag gagcagcaga
     5101 gacatcagtg gaacagcgcc aacaggaagt gagtatgtca acgtgcattc gagcgaattc
     5161 ctcgggatgt gcccgccgaa gctggtgatt aactatcgga accagtcgaa gataaagaac
     5221 acaggttcca ctaacatcaa aggctatctc cttatccaga ttcagttcta caacacttca
     5281 caagcaaaat gggtaatcga taatgatacc gtaaacgaga gcatacccag ggtcatcaac
     5341 gcaggtggtt ctggttctgg tagtcaactg ggtcttgata ccatcttcaa cagactagtg
     5401 agagcatcag atttaacaca cggcacagga acctatcgag tttatactgc tttcagagat
     5461 cctgaaggga acaacctgaa aacgaatgat gatgtggacc ttgaagcctg gtggcaattc
     5521 agcaaaacct aacgaaaaaa ctaatccatc ataaaatgga agtcaggtag ttcacacctg
     5581 acattctata ctttttttta aaaagatgca ggatactgaa taaaaagtgc tgagacattc
     5641 catgatatta tatagaatcg ctctggataa gaccatacta gtttttatcc agcgcatcat
     5701 tcctttactg tctcatattt cagttcatca tgtatttctt aacaatatca agttatcaag
     5761 gcatcacagc aatgatgaat taactggagt aataaaatga acacgttaga tactaagata
     5821 aaagaacttg tttattttct taataccctt ccaacggtta aaacaatagg aagttgtcaa
     5881 gggcacgatg atgggggaga aacagggaat tttgatgtcc cgtatatttc atttgcttgt
     5941 atcgacagaa gggtattggg gttcttagcc agcattcgat ttgcgtatga agatccttct
     6001 gatatattac caaaatttag gaagaatcaa ccacggttgc aggctgattg gagtattcat
     6061 gtaattactg gagaagatcc agaatattca ccagaggaac ttaaagcaga taatcgacag
     6121 gaagattatg tcttttatgt attacaacca gacagatatt cttatgataa gccctctgat
     6181 atttatggtg atttctcaga gattttaaag tattatcagt ggaaatggaa gagaatgaaa
     6241 tgaaattagg attccatcca tataacccta tacttttcgt tccatggcag actcgttata
     6301 aaaaacttta aagtggaaat caggtatctc acctgatttt caatattttt tttgtttaag
     6361 aggatataag gaaaatattg gaaaaattta cttataaaca ggttggcaac aattaaatac
     6421 tgatagatat gtgcatttac tggtgaagtg caaaatggaa acatacaaaa gtaatgaaaa
     6481 aaagttactt attgtacttc ccacggtaga ttcaaaaata tttaaatacc atcacttgat
     6541 ttttatctta acaacttcta ccagcaaact cattctttcg ttagctattt atactccttt
     6601 ttacactctt ttgtgatgac acgacatttg atatcaatga attaataaag atttgttttt
     6661 taagaataga aaaatcatga gattgataca ggattgaatt attacgttgg ccaaatgcga
     6721 atcgttttac tggggagcgt ttcattatgt tttgttatca aacctgtttg ttgaagataa
     6781 atacccttag cttttgcagt gattttcctc acgttttttc ttgcttttaa tatcaacata
     6841 ggattgaaac tctcgacagt gtcagtttca taaatcaacc aagatacctt ataatttttt
     6901 ggtccagatg tgttaatcat gcatttcttt atagtccatg aaaatggaat gggatcgaga
     6961 tcgattttta ttatttttga taataattta attgaggttg aaggaacaaa atttaaaagt
     7021 tcgttgacac caagatatat cttaccgcta atgattttta tatctttaaa ttgatctttt
     7081 ggtgctacat ctactgctgt cacgtcagtt ctatcattag tattgcactt taaatcaaca
     7141 ttcaatcgca gtttagggac cgttattcct ctcgctaaca ttacatttaa acctaattca
     7201 acaataaaga agttgaattt tgaaaaatct tttttaattt gatcagctac tttaggttca
     7261 tttggtggaa tctgatacac attagc
//
LOCUS       DATMXJ010000126         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_75814, whole genome shotgun sequence.
ACCESSION   DATMXJ010000126 DATMXJ010000000
VERSION     DATMXJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75814"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VN377_03435"
     CDS             <1..1780
                     /locus_tag="VN377_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27473.1"
                     /translation="QYINITDPPNPLENLTVIDRGLTWNKYYNATSGCNELEVSTSPI
                     NYQEDNGTWTPINNSFYQLPSDHPAYNHGYRVGNDHGLFGVYFKPNVQNDWPVSFTYN
                     RSDDPTVHVIRSKLVGVGYVDPQSNWAYQYLQNVQSSQGQTNGNTINYPSVFTGIDIT
                     WSYDNTELKEEIRLSNMTKTVLQNHPPSQYGLQDASSYLVFITKLDHQNLNLYNNSEL
                     LSGNVTISNAGVEFRDALGYFKCALPLGEAYELNNESVRQKLTFRIIQYKGNTYLLSG
                     LKISDLTAMTFPVVIDPTLSVNSLSNDGYIYNFNTNYNTAWSASSGTIDSSATYLTIG
                     QKSNGGIPNTYYIYRGFVLFNTSTLPSNAYIDSAILSLYKKDDYSTTDFQLTIQNGQP
                     IYPHNPLQTGDYGKSHYSGNGGSLNTANFVNGRNNITLTNLNWINKTGTTKLCLRSSR
                     DINGNSPSGAEYINVYSANAPLGGQVPKLIITYRNQSKIKNTGSTNIKGYLLIQIQYY
                     NYSQGKWIVEKDTVNETSTRTINTGQQLALDTIFNGHVRISDLTHNDSTYRVYAAFRD
                     PIHNILRTNDGKELVAWWQFLGPQTG"
     gene            complement(2182..>2519)
                     /locus_tag="VN377_03440"
     CDS             complement(2182..>2519)
                     /locus_tag="VN377_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HWR27474.1"
                     /translation="EVQGKEVRFFLFDKGFVKTLEERNHLLEEASLHFHVSKEKVEEA
                     MFSDLEEEQILVEFTAPTPEELVRWYNLSLTQTLLFDALELTFKIDGNYQQVSRYVKL
                     KNYFTNIKY"
BASE COUNT          773 a          638 c          465 g          643 t
ORIGIN      
        1 ccaatatatc aacatcacgg atccaccgaa cccactagag aatctcacag tgattgatcg
       61 aggactcacc tggaataaat actacaatgc cacctccgga tgcaatgaac ttgaggtctc
      121 caccagtcca ataaattatc aagaggataa cggcacatgg acgccaatca acaattcctt
      181 ctatcaactc cccagcgatc atcccgccta caaccatgga tatcgagtag gaaatgatca
      241 tggtttattc ggcgtctatt tcaagcccaa tgtgcagaat gattggccgg tctctttcac
      301 gtataaccgg tcagatgatc ctaccgtcca tgttatcagg agtaagcttg tcggcgtcgg
      361 gtacgttgat ccgcaaagca attgggcgta ccagtatctg cagaatgtgc agagcagcca
      421 gggacaaacc aacggtaata caataaatta tcccagtgtc ttcaccggga tcgacattac
      481 ttggagctat gacaatacag aactgaaaga agaaatcaga ctgagcaata tgacaaaaac
      541 cgtgttacag aatcacccgc catcacaata tggtcttcaa gatgcaagct catacctggt
      601 ttttatcacc aaactggacc atcaaaacct caacttgtac aataattcgg agctactttc
      661 cgggaatgtc acgatatcca atgcaggggt cgaattcagg gatgcacttg gatatttcaa
      721 atgtgccctg cccctcggtg aagcatacga actcaataac gaatcagtac gtcagaaact
      781 cacattcaga ataatacaat acaaaggcaa cacctatctc ctctctgggc taaagatatc
      841 cgatttgact gcgatgacgt tccctgttgt catcgaccca acgctctccg tgaactccct
      901 atcgaatgac ggatacattt acaatttcaa tacaaattat aacaccgcat ggagcgcatc
      961 cagcggtaca atcgacagct ctgcaaccta tctaaccatc ggacagaaat caaacggagg
     1021 catccccaat acatactata tctaccgtgg gttcgtcctc ttcaacacct caacactacc
     1081 ctccaatgcg tacattgaca gcgcgatact ctcattatat aaaaaagatg attactcgac
     1141 cacggacttc caactcacca tacaaaacgg acagccgatc tacccgcaca acccgctcca
     1201 aacaggcgat tacggtaaga gccactattc gggtaatggc ggcagtctga acaccgcgaa
     1261 ctttgtgaac ggacggaaca acatcacact caccaacctc aactggatca ataaaacagg
     1321 aacaaccaaa ctgtgccttc gcagcagcag ggacatcaac ggaaattcgc cctctggggc
     1381 tgaatatatc aatgtatatt cagccaacgc ccccttagga ggacaagtac caaaattaat
     1441 catcacctat cggaaccagt caaagatcaa gaacaccggg tcaacaaaca tcaaaggcta
     1501 tctcttgatt caaatccagt actataacta ctcgcaaggc aaatggattg tcgaaaaaga
     1561 caccgtaaac gagacttcga caaggactat aaatacaggg caacaactcg ccttggacac
     1621 cattttcaac ggtcatgtga gaataagcga tcttacccat aacgacagta cttatcgtgt
     1681 ctatgcagca ttccgggatc ccatccataa catcttgagg acaaatgacg gtaaggagct
     1741 tgtagcctgg tggcagttcc tcgggcctca aacagggtaa tgcattcaaa gaaaattgaa
     1801 acaccagatc aggtaaaaat acctgattct ctttttttct ttttgtcaat ttagtgtttg
     1861 caaattgttt caataagtag tcacaccact attttgacac catagatttt ccgcccctca
     1921 actatacagt acccatcatc tcaattggat taaaaagcat gatgctgtcg atttcgcttt
     1981 ttccatagct tgtctaggat tcatcccatt tcggatgttc tcaaaataag agtcgatgaa
     2041 aatctttttt attaaatcaa attttttctt attgccattt tcatgaagga agtttgtaaa
     2101 ctctctcagc cattctggat cgagttgttg ttgaccctct gaagattgac tcatctgtat
     2161 ctctgttata ctttgtcatt tctaatattt tatattggtg aagtagtttt tcaatttgac
     2221 gtaccgggac acctgctgat agttaccatc tattttgaag gtaagctcca gggcatcaaa
     2281 caacaacgtc tgcgtcagcg acaggttata ccatcgtacc aattcctcag gtgtaggagc
     2341 ggtgaactct acaagaatct gctcctcctc cagatctgag aacatcgcct cctcaacctt
     2401 ctccttggaa acatggaaat gcagagacgc ctcttccaag aggtggttcc gttcctccag
     2461 ggtttttacg aatcctttgt caaacaagaa aaaacgtact tcttttccct gtacctctg
//
LOCUS       DATMXJ010000127         8472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7787, whole genome shotgun sequence.
ACCESSION   DATMXJ010000127 DATMXJ010000000
VERSION     DATMXJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7787"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1069)
                     /locus_tag="VN377_03445"
     CDS             complement(53..1069)
                     /locus_tag="VN377_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27475.1"
                     /translation="MLEKLRSFADIGRTQGITTSASIAIVGALTSTVPVEWYYIVFFT
                     IISIFSHMALNTYIALGDVELDAHTYVPSRNPVVSGLLSRREAMLFVYGGTLVCLLMI
                     AFLLFSLDTLSVLMCFLCFVPAYGSLLWYGWKGKKILISYDFSFSISYSFFVLFGVFV
                     VGGMPTIYTWIFIGVVIFAATAFAQWENGLKDVDADRSVGVKSFAVITKVKNNQRLHG
                     THPYFLYGCGLKIGFLLFSFLGYWYSRNISYLLFFVVYGIPSQLYIMYRFLVKQKPQE
                     HRRTILLDVTFAAILGYSTIYGYTGIIPIVLLIIYLIGGYLIGSLIQSNCEFKFGRFS
                     FSSK"
     gene            1128..2150
                     /locus_tag="VN377_03450"
     CDS             1128..2150
                     /locus_tag="VN377_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HWR27476.1"
                     /translation="MDLRFTFVVMLRDYIEITRLFNMGLTAIAPVLGALSMWDVGSLS
                     LWKLFILFIIGSLAHIYGFVINDVIDIRLDKLSKDLSARPLVRGSITRRRATVFAISC
                     MIGSFLLSLVFFSNLLRYLVIAFILLIAYFLATMYDIASKKYPGMDLVVAGAIFFLIL
                     FGASTIGTPTMLTYVVALIGGIQVLFMNMINGAIKDIDHDEEGSANTVAIRLGAKIYA
                     GVIALPLSFKTTGYLIEIARIALIFLPFVFLWLSPSLWQIALLVLFAIATFISIYKLY
                     SIKTFDRARIRKLIGIIVIFMYASVPIMLSSLNIYIILVALIPPLWFFASNLVLHKTI
                     FEPKTM"
     gene            complement(2156..3781)
                     /locus_tag="VN377_03455"
     CDS             complement(2156..3781)
                     /locus_tag="VN377_03455"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWR27477.1"
                     /translation="MVNYIIVTGGVISGLGKGITTASIGKILVNHGYKVTAIKIDPYI
                     NFDAGTLRPTEHGEVWVTEDGGEIDQDLGHYERFLDVNIPKCNNITTGQVYSAVIEKE
                     RNGKYLGKTVQPIPHVTDEIKRRIRAPGEEAKFDFVLVEIGGTVGDYENVLFLEAVRQ
                     MKHEGEKVIYVHVTYVPVLKSVGEAKTKPTQHSVKLLREIGIQPDFIITRSEKPLDDV
                     RKEKIALFCNVSGEDVISDSNIDNVYGVPLLFEEQQLCKKILKKLGLRKEENDFNTWK
                     NYIAKIRKMDKKVKIGIVGKYFDIGAFKLSDSYISVIEAIKHAAWNNNVRPEIEWIDS
                     KIFEKHPRKIVNLKKFDAIIVPGGFGLSGIEGKIATIKYARENNIPYLGLCLGMQLAV
                     VEFARNVCGLKDANTTEVEKNPPYPVIDFIPDQVKIVTESRYGATMRLGAYPAILSDG
                     SLIKKLYNDQNKVYERHRHRYEVNPKYIEILEKKGLVFSGRSPDGILMEFMELPSHPY
                     FTGTQAHPEFKSRPMKPAPLFDGLIKAAKKKKE"
     gene            complement(4261..5985)
                     /locus_tag="VN377_03460"
     CDS             complement(4261..5985)
                     /locus_tag="VN377_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27478.1"
                     /translation="MKHVWWKLSILMISVIIFSPNANPFVFSDSGKIMIDNHAQYIFE
                     YKTNWYEKNNNFIQDTITSISDFQKVQKTFVESNNIPANYDKDGPMDSAWPMFSHDVA
                     HTGRSPFSTDNNSGNEIWYVRSDKDGAVWGSAVIDNDGIIYFGTTGSDASIYALYPNG
                     TQKWQFFASGALWVSPALAENGDIYCTTWGSIDYFQAITKNGTERWHFHQEGSSASAP
                     TIGSDGTIYFGTDSHNIFAVNPSGAEKWRYTTGYIVMSSPSIGEDGTIYIGSCDHYLY
                     ALNPNGTLRWRFGTGNEIKGSASIAQDGTIYVPSFDGYLYALYPNGTMKWRTSTGSSV
                     AAAGVALADDDTIYIGTEKLRAYNPDGTLKWITDLQGSIYGSIPAVSADGTIFVSAGG
                     SLVAVNPDGTIKWRNHLSLVQIKSSPVIGPDDRVYVGSEAPGLTPPGYLHAIGKKDAN
                     APTNPTISGEIEGKIHRKYEFTFTSTSPLTNNLFYQIDWGNGTLTDWLGPYSSGESIT
                     LSHSWSEKGTYTIRARAKDTENLWSSWGTLSVTMPFSYDLPILRFFDRLLERFPNAFP
                     FLRQIMGY"
     gene            complement(6192..6368)
                     /locus_tag="VN377_03465"
     CDS             complement(6192..6368)
                     /locus_tag="VN377_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27479.1"
                     /translation="MELNISEKVGSLLQAVFLLILPWVVLLIGLAIPLESVWFYLLCI
                     TWFGCGMVFFETLN"
     gene            complement(6427..7176)
                     /locus_tag="VN377_03470"
     CDS             complement(6427..7176)
                     /locus_tag="VN377_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWR27480.1"
                     /translation="MQPALQPLIDEPALLLKEKKLLVIADLHIGIESELRENGLQVPS
                     QTKIMEERLITIITKNKIDDIIFLGDIKHTIPSSTFQERTDVKKFLETIHSYCTLHIL
                     PGNHDGNIDRLLTSGIQLHPSNGYVFEGIGFAHGHRKPSDEIMQCDQVIIGHSHPTVM
                     LMDRLGYRTFEQCWLRGPILSDVLKEKYPNSPTSQILLMPAFNPLCGGTAVNQDVLLG
                     PFGPLIDVDNADLYLLDGSSIGKVKDLKNSS"
     gene            complement(7180..>8472)
                     /locus_tag="VN377_03475"
     CDS             complement(7180..>8472)
                     /locus_tag="VN377_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27481.1"
                     /translation="ITTAVSLEDFMMQYYDQITSFSDVDIITDNGLFLLGINDGTITL
                     SSDLPYAVTTVVPLEIVPDTITRFFVLGTDLPLSVHCNGDFSILTTLSDTSTISIRAR
                     NGETLWTGGSPNDYLIIQDTTFSFTKNPPLSFFPLHNTTSTISLTVSVSPAASNDVRI
                     QQLIQNVSTVLGDFEDGISPDFVQSINDLDASLQAASFIANGVMVFLRTNDSVTIDHT
                     TQRFSSGGFVRFNTLEITNFEFSSGPVIKADSTLCFLGDHFYNPSAKRSVDGISFPYE
                     LFFIWILALCVFVYVRFFLRPPIDITLDAKIKRYALFTHLFALVIAFLLLDMEVNILF
                     GISALTGLLSQGLIMSTAVFFVLEIIIWMLGYLILAIPLQLLSYAILRFLGIGKGGNG
                     LRKAVGDLSIWVFCGFYLLLFFNIIFSLFDFNSLLPMG"
BASE COUNT         2486 a         1728 c         1813 g         2445 t
ORIGIN      
        1 gagctgctcg aacgattgat gcgtatttga aaaacacctc gtagaatgtt ttctatttgg
       61 atgaaaatga gaaccttcca aatttaaatt cacagtttga ttgtatcaat gaaccgatca
      121 ggtatcctcc aatgaggtag ataatcagca gcactatcgg gatgataccg gtgtaaccgt
      181 agattgttga gtacccaagg atagcagcaa acgtcacatc aagaagaatc gttcttcggt
      241 gttcttgtgg tttttgtttt actaaaaatc gatacatgat atagagctgg gatgggattc
      301 catacaccac aaagaaaagt agataggaga tgtttctgga gtaccagtac ccaagaaaac
      361 taaagagtag aaaaccgatt tttaatccgc atccatagag gaagtatggg tgtgttccat
      421 ggagtctttg gttgtttttc actttcgtga tgaccgcaaa gctttttaca ccaacactcc
      481 ggtcagcatc aacgtctttc agaccgtttt cccattgggc aaatgccgtt gctgcaaaga
      541 tgaccactcc aataaaaatc caggtatata tggttggcat tccaccgaca acaaaaactc
      601 cgaagaggac aaaaaaggaa tacgatatcg aaaatgaaaa atcataggag atcaagattt
      661 ttttcccttt ccaaccatac caaaggagcg atccataggc agggacgaaa caaagaaaac
      721 acatgagaac tgagagagta tcaagagaaa aaagtagaaa ggcaatcatg aggaggcaga
      781 caagggtccc tccatagacg aagagcattg cctctcgtct cgacaaaaga ccactaacga
      841 cagggtttct tgagggaaca taggtatgcg catcaagttc cacgtcgcct aacgcgatat
      901 aggtgttcaa tgccatatgt gagaagattg agataatcgt aaaaaacaca atatagtacc
      961 attcaacagg tactgttgat gtcaatgcac cgactattgc aatggacgcg ctggtggtga
     1021 tcccttgtgt ccgtccgatg tctgcaaacg agcgaagttt ttctagcaca gaagaaaaaa
     1081 atagaaatcg gtataaaaag ctataggtta caaaccgatg gttttttatg gacctccggt
     1141 ttacttttgt ggtaatgctt cgtgattata ttgaaataac acgtcttttc aacatgggct
     1201 tgaccgctat cgctccagtc cttggggcgt tatccatgtg ggacgttggt tcgttgtctc
     1261 tttggaagtt gtttattttg tttatcatag gtagtctcgc tcacatctat ggatttgtga
     1321 ttaatgatgt catcgatata agactcgata aactctcaaa agatctctct gcacgaccat
     1381 tggtccgagg aagtatcact cggaggcgtg ctacggtctt cgcgatatcc tgtatgattg
     1441 gttcgtttct cctttcgtta gtattttttt ctaatcttct cagatacctc gtcattgcct
     1501 ttatcctgct gatagcttat ttcctcgcga cgatgtacga catcgcaagt aagaaatacc
     1561 cagggatgga cctggttgtt gccggtgcta tttttttcct gattttattt ggtgcatcga
     1621 cgattgggac tcctacgatg ctaacctatg ttgtggctct gatcggagga atacaggttc
     1681 ttttcatgaa tatgattaat ggtgcgataa aggatattga tcatgacgaa gaagggagcg
     1741 cgaacacggt tgcgattcgt ttaggggcaa agatctatgc tggagtcatt gccttaccgc
     1801 tttccttcaa aacaactggg tatctgattg agattgcacg gatagccttg attttcctgc
     1861 cgtttgtgtt tctttggctg tctccaagcc tttggcagat agcgttgctg gtgctgtttg
     1921 cgattgcaac gttcatttcg atttataaat tgtattccat caagaccttt gaccgagcac
     1981 gtatcagaaa attgattggg attatcgtta ttttcatgta cgcatctgtc ccgataatgc
     2041 tcagttcatt gaacatctac attatccttg ttgcgttgat cccaccattg tggttcttcg
     2101 caagtaatct cgtgttgcat aaaacaatat tcgaaccaaa aacaatgtaa tggttttatt
     2161 ctttcttttt cttggctgcc ttgataagac catcaaagag aggagcgggt ttcatcggcc
     2221 gagatttgaa ctccgggtgt gcctgtgtac ctgtaaaata aggatgactg ggtaattcca
     2281 tgaactccat caggatccca tcaggggaac gaccggagaa caccaagcct tttttctcaa
     2341 ggatttcgat atatttcgga ttgacctcgt aacgatgacg atgtcgttcg tagaccttgt
     2401 tctgatcatt atataatttt ttgattaatg agccatcgga gagtatcgct ggatatgcgc
     2461 ctagccgcat ggtggcacca taacgggatt ctgtcacgat tttcacttga tcagggatga
     2521 aatcaataac tgggtagggt gggttttttt caacctcggt ggtgttcgcg tcttttaacc
     2581 cacagacgtt tcgtgcaaat tcaacgacag caagctgcat cccaaggcat aatccaagat
     2641 agggaatgtt gttctcacga gcatatttta tggtggcaat ctttccctca ataccagaga
     2701 gtccaaaacc accagggaca ataatcgcat caaatttttt caggttgaca atcttgcgag
     2761 ggtgtttttc aaagattttc gagtcaatcc attcgatctc cgggcggacg ttgttgttcc
     2821 acgcagcatg ctttatcgcc tcaatgactg aaatatagga atcagaaagc ttaaatgctc
     2881 caatgtcaaa atatttcccg acaatcccga tttttacttt tttatccatt ttacggattt
     2941 tcgcgatata attcttccag gtgttgaaat cattctcttc tttgcgaaga ccgagtttct
     3001 tcagaatctt cttgcaaagc tgctgttctt cgaacagtaa ggggactccg tagacgttat
     3061 cgatattaga atcagagatg acatcctcac cagagacatt acagaacaaa gcgatctttt
     3121 ctttccgtac atcatccaga ggtttttcag aacgggtaat gatgaaatca ggctgaatcc
     3181 ctatttctct gagcagttta acggaatgtt gggttggctt ggtttttgcc tcgccaactg
     3241 atttgaggac agggacgtaa gtgacatgga cgtagatgac tttttctcct tcatgtttca
     3301 tctgacggac tgcctctaaa aacaagacat tttcatagtc tccaacggtg ccaccgattt
     3361 caacaaggac aaaatcgaat tttgcttcct ccccaggtgc acggatccgt cgtttgatct
     3421 catcggtcac atgagggatt ggttgtacgg ttttcccaag atatttcccg tttcgttctt
     3481 tttcaatcac cgcgctatag acttgaccgg tggtgatatt gttacatttt gggatgttga
     3541 catctaaaaa gcgttcataa tgaccaaggt cctggtcgat ttcccccccg tcctctgtga
     3601 cccagacctc accatgttca gttggtcgta gggttccggc atcaaagttg atgtagggat
     3661 cgatcttgat cgcggttact ttgtatccgt gattcacaag gatcttgcct atcgatgcgg
     3721 tcgtaattcc ttttcctaaa ccagaaatga ctccacccgt gacgataata taattaacca
     3781 tcccgatcaa caggaaaaca gaataaggta ggtgatttaa atgatttttg actgttcata
     3841 cagtgaattt aaatcacact tttccttctg ttttccccct ccaaactaat agtatataaa
     3901 tctaattcac taaatgggtt aatcgttgtc ataaacgctc cagcctccaa agttggagaa
     3961 ggaatatgat gctcgctata taactattcc tttttatcga aaaatgcatt aacacgtttt
     4021 ttaacccatg gttttttgtg cgaatcaaaa tgataaaaaa gatgaaaaaa ggaaagaaaa
     4081 agtggcctat cgtgtaacca gttgtttcaa cgcattatat tttcctcaaa ttattgagaa
     4141 caatattttg accgttacat taaaagcccg aaccgggctc actatccaaa ggttcaaaaa
     4201 atattattcc catcgagaaa gcccttcaaa aaaaggaaaa aaaattggag gaaatttttt
     4261 ttagtacccc atgatctgtc ggaggaatgg gaatgcattc gggaatcgct ctagtagtcg
     4321 gtcgaagaat cttagtatcg gtaaatcata tgaaaatggc atggtaaccg agagagtccc
     4381 ccaagagctc cagaggttct cagtatcctt tgctcgtgct cgaatagtat aggttccttt
     4441 ttcagaccag gaatgactca aggtaatcga ttccccagaa ctgtatggtc caagccaatc
     4501 ggttagcgtc ccattccccc aatcaatctg ataaaaaaga ttatttgtta atggtgaggt
     4561 ggaagtgaat gtgaattcat attttctgtg tatttttcct tcaatttcac ctgagattgt
     4621 cgggtttgtt ggggcgtttg catccttttt tccaatagca tgaagatatc ctggaggtgt
     4681 caatcctgga gcctcagagc cgacatatac acgatcatca ggaccaatga ctggcgagga
     4741 cttgatctga acaagtgaca gatgatttcg ccatttgata gttccatctg gatttacagc
     4801 gactagagag ccaccagcac taacaaagat agtaccatct gctgataccg cagggatact
     4861 cccatagatg gagccttgta aatcggttat ccatttcaat gtcccatcag ggttatatgc
     4921 acggagtttt tctgtcccaa tgtaaatcgt gtcatcgtcc gcaagtgcga cacctgcagc
     4981 agcaacgcta ctgccagtgg atgttcgcca tttcattgtc ccattgggat ataatgcata
     5041 aagatatcca tcaaacgagg gaacatagat agtaccgtct tgtgcgatag atgctgatcc
     5101 tttaatttcg ttacctgttc caaaccgcca gcggagcgtc ccattagggt ttaatgcata
     5161 gagataatga tcacaagatc caatatagat agtaccatct tcacctattg atggagagct
     5221 cattacgata taaccagtgg tgtatcgcca tttttccgct ccagagggat tcaccgcaaa
     5281 aatattatga ctatcggtcc caaaataaat tgtaccatcg gatccaatag ttggagcaga
     5341 tgcagagcta ccctcctgat ggaaatgcca tcgttctgtt ccattttttg tgatcgcttg
     5401 aaaataatct atggatcccc atgtagtgca ataaatatcg ccattttccg caagagcagg
     5461 acttacccaa agtgcgccgc tggcaaaaaa ttgccatttc tgtgttccat ttggatataa
     5521 tgcatatatg gaagcatctg aacctgttgt tccaaaataa ataattccat cgttatcaat
     5581 taccgcggaa ccccaaacag caccgtcctt atcactacgc acataccaaa tttcattacc
     5641 agaattatta tctgtactaa aagggcttcg gccggtatgg gctacatcat ggctgaacat
     5701 cggccaggca gaatccatcg gaccatcttt atcataattc gccggaatat tatttgattc
     5761 tacaaaagtc ttttgtactt tttgaaaatc agaaatactt gttatagtgt cttggatgaa
     5821 attgttgttt ttttcatacc agttggtctt atattcaaaa atatattgtg catgattgtc
     5881 aatcataatt tttccgctat cactaaaaac aaaaggattt gcatttggac taaagatgat
     5941 aacggatatc atcaatattg aaagtttcca ccaaacatgc ttcatttatg aggttcccaa
     6001 gatgataata tccaagaact tctttaataa tcttttcata aaaaaagtga atactttctc
     6061 gacggtcctc atttgaataa ctgacaaaaa ctcgtaaaga ttttcctacg aaaatttttc
     6121 tttcgtctat aatttaatgg tagataaata tgattttggt tttaatgaaa aacgagtgat
     6181 tcattctcac gctagttgag ggtctcaaaa aagaccatcc cacaaccaaa ccaggtgata
     6241 cataagaggt aaaaccagac actctccaat gggatcgcaa ggccaatcaa gaggacgacc
     6301 caggggagaa taagaaggaa taccgcctgt aataggctgc cgactttttc agagatattc
     6361 agctccatag gtcatcagta tctgttgatg taagtcggtg tcgtatattc catgatgtat
     6421 ttaaggttaa cttgaatttt taaggtcttt taccttacct atcgatgatc catccaaaag
     6481 atacagatct gcattatcaa cgtcgatgag gggaccaaac ggcccaagta acacatcttg
     6541 gttcaccgcg gtccctccgc ataaaggatt gaatgcaggc atgagaagga tctgcgatgt
     6601 cggtgagttc ggatattttt ctttcaagac atctgaaagg attggacccc gtaaccaaca
     6661 ttgttcaaac gtccggtatc caagacggtc catcagcatc acggtagggt gggaatggcc
     6721 aatgatcacc tgatcacatt gcatgatttc gtcagatggt tttcgatgac catgagcaaa
     6781 tccgatccct tcaaacacat atccgttaga gggatggagt tgtatacctg atgtgagcaa
     6841 ccgatcgata ttcccatcat ggttacctgg caagatgtga agagtgcagt atgaatgaat
     6901 cgtttcaaga aattttttca catcagtccg ttcttgaaag gtcgaagaag ggatggtatg
     6961 tttaatatct ccaagaaaga taatatcgtc tatcttattt ttcgttataa ttgtgatcaa
     7021 tcgttcttcc attatcttgg tctgtgatgg tacctgcagg ccattctctc gcagttcact
     7081 ttcaatacca atatggagat ctgcgatgac caggagtttt ttttccttta gcagaagagc
     7141 aggctcatcg atgagtggct gtagtgctgg ttgcataggt tatcccattg gcaagagact
     7201 attaaaatca aacagagaaa aaataatgtt gaaaaataag agaagataaa atccgcaaaa
     7261 cacccagata gacaggtctc caacagcttt ccgtaaacca ttaccacctt tcccgatacc
     7321 aaggaatcgg aggattgcgt acgacaaaag ctgaagtggg attgccaaaa tgaggtatcc
     7381 taacatccag atgatgattt ctaagacaaa aaatactgcg gtagacatga tgagtccttg
     7441 cgagagtaga ccggtaaggg cgctgatacc aaagaggatg ttgacctcca tgtcaagcag
     7501 gagaaatgca atgaccagag caaaaagatg agtaaagaga gcgtatcgtt taatcttcgc
     7561 atctagcgtg atgtctattg gaggtcgtaa gaaaaaccgt acataaacaa agacgcacag
     7621 tgcaagaatc catatgaaaa acaattcgta cggaaacgag attccgtcaa cgcttcgttt
     7681 cgctgaaggg ttatagaagt gatctccgag aaagcatagg gtgctatctg ctttgatcac
     7741 cggccccgag gaaaactcaa aattagtgat ttcaagggta ttgaaacgaa caaacccccc
     7801 tgaggaaaac cgttgcgtcg tatgatcaat agtcacggaa tcattggtcc gcaggaaaac
     7861 catcactcca tttgcgataa aagaggccgc ctgaagagaa gcgtctaagt cattgatgct
     7921 ttgaacgaaa tcagggctaa taccatcttc aaaatccccg agaaccgtcg agacgttttg
     7981 tatgagttgt tgtattcgaa cgtcgttgga agctgccggg gaaactgaca cggttagtga
     8041 gattgtcgac gtggtgttat gaaggggaaa aaaagataat ggtggatttt tagtaaaaga
     8101 gaatgtcgta tcttggatga tgagataatc gtttggcgac ccaccagtcc agagtgtctc
     8161 accgttcctc gctcttatgg aaatagtgct ggtgtctgat agggtggtaa ggatagaaaa
     8221 atccccattg caatgcacgc tgagcggaag gtccgtacct aagacaaaaa accgagtgat
     8281 cgtatcaggg actatttcaa gagggacaac ggtagtaact gcatagggga gatcagagga
     8341 tagggtgatc gtaccatcat tgatgccgag aagaaacaga ccattatcag tgatgatatc
     8401 aacatctgaa aaagaagtga tctggtcgta atattgcatc atgaaatcct caagggaaac
     8461 agctgtggta at
//
LOCUS       DATMXJ010000128         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55012, whole genome shotgun sequence.
ACCESSION   DATMXJ010000128 DATMXJ010000000
VERSION     DATMXJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55012"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VN377_03480"
     CDS             complement(<1..210)
                     /locus_tag="VN377_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWR27482.1"
                     /translation="MVLDNLGDSLKRTIKKIANAVHVDAPLVKEIVRDIQRALLQSDV
                     NVKLVLELSKNSRSSIPFNVWSNMIW"
     gene            332..2590
                     /locus_tag="VN377_03485"
     CDS             332..2590
                     /locus_tag="VN377_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HWR27483.1"
                     /translation="MNSTNKIIGIGVVSLFIITSFSGCVQQPGPGSNLIIIPSVDIIS
                     SDNPKYKMASYYSSEEITIDAQTPQYSFPLDLSTIQNLNDIDSVFSLTVQQEELLKRN
                     GFFVKDFGVEDDITDPYNYLKDHEIPVFVTSDTLLHLYHIMFDQTLKGIEEREFFDSI
                     LDLSKALFDKSIEDYDTFTEPHLKEAARRNVGFFGVALSLLQTPTTGYNNSETIRTVS
                     FSIPEYVTENVTTELSFIEAHEGFHESSLFRYKEDYSQYNPRGHYTQTEKLKRYFKAM
                     MWYGRITFLLRGSDIVSEVDANIATIQACLISTALPALTDDGRPLDELWGRVYVVTTF
                     FVGSADDLIPSEYLTSINIVFGARFDATEFTNETKMVNLLGALAQQRSPQIYGGTGNA
                     DIQPPFSKEQLNEVLEKTKGMRLMGQRFIPDSYMFQNLVFPTIGQFMGSGEPFTYWMG
                     QRVMPRGLDIMTILGSKRARELLNIEGDTAYEFYDRQVDNLTGQFALLNTTEWNRNLY
                     WGWLYTLQPLLGSFDTRYPSFMQTNAWSDKELQTTLASWTELRHDTILYAKQSYTPRI
                     TSIPPEETPGSGYVEPVPEFYQRLLALTTMTRTGLTDLQALNETETYRLNNLETLLRR
                     LLTISALELKGKGLNESDYEFIHTFGEQLDTIITGVNDQGKETTLIADVHTDTNTASV
                     LEEAIGYVDLIIVAYKTPNGNIIVGAGPVFSYYEFKQPMADRLTDEAWKQLLEAGQQP
                     SRPIWTESFIAE"
     gene            complement(2594..3316)
                     /locus_tag="VN377_03490"
     CDS             complement(2594..3316)
                     /locus_tag="VN377_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HWR27484.1"
                     /translation="MRVTCTYPCYRVNKKPSSKAGKKRENLKLVFFDMDGVLLDTVSS
                     WRYIHEYFGTTNERSIMPYLRGDIDYLEFIRRDVSLWKIDGKAVSKATIEKILYSIPF
                     INGVEDCISFLKEHGVQTAIVSAGLDILAEKIAADIGIDYVFANEVKVGPDGRLTGEG
                     VLHVELMQKDKNVKELVQKLHLSLDDCAAVGNSCFDIPMLEVCGLGIAFNPEDACIKQ
                     CADVVVDEKDLRKLIPTFQTYL"
     gene            3485..4993
                     /locus_tag="VN377_03495"
     CDS             3485..4993
                     /locus_tag="VN377_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27485.1"
                     /translation="MKRLRSARMKKSIVFFIAVFGFLIGPSLMAHNTYLSDDNNDDKT
                     GSSAEPSPVIYDLLIIAPKKYSNALQPLIVHKNRVGIKTNLVTLDQVYDQMFWHGRDK
                     QEKIKYFIKTAKEEWGITYVMLVGDFQTIPIRYVYNTDNNTGWDEPCFISELYYADLY
                     DKNHNFSTWDTNNNGVYGEWYGEMAKDPDIDLYPDVFVGRLACMNEAEVRVMVRKIIT
                     YETTAYGQTWFNRFVVVAGDTYPESENPNWTGYEGEQNTMDAIENMSGFEPIKLWTSD
                     ESFTGPKDVIRAINQGCGFLLFEGHANAFSWSTHPPKDAQTWIKGLNTVSMERLRNRQ
                     MLPVCIVGGCHNNEFDVTPANIIKGLKEEGLRYFSSGSIPFGSFWRYTWIRECWGWKL
                     TRINTGGSIATIGCTGLGMSKEDKESFSGAGDYLEPTFFHEYGVNGTRILGKVWGNTI
                     SDYLNHYPIDWNTPAAKDSAIDAKTVQQWALFGDPSLRIGGYSASELISTLG"
     gene            5036..6469
                     /locus_tag="VN377_03500"
     CDS             5036..6469
                     /locus_tag="VN377_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27486.1"
                     /translation="MNTQKIRTITTVGILFLFFGSIIGPLGTATAADTQMVTEQKESH
                     QAPLSIYSLLIITPNIFKNELQPLVRHKNSHGLSTILVTLPEVYHQMYWYGRDDAEKI
                     KYFIKTAMEEWGIRYVLLVGGRSSQFLPTWYCPVRYVSMVDDWDVGYLSDLYFADIYD
                     ADGNFSSWDSDDDGLYGEWYIGETAQDVAFDLTPDVAVGRLPCRSEREVRIMVRKIIT
                     YETTVLNQPWFSTMLVAAGDTYPETSNANWTGYEGEYYGDRAIENMTGFTATRLYTSD
                     QTFLDKKNVIETFRNGWGFVYFVGHGSPKQWGNNAPNGSEFIQGPNSNDMWRFRNKDR
                     LPVCVVSGCHNSQFDVCLRRLFNYSTRWKQEYVPECWSERIIREPSGGSISCIGCTGL
                     GYTKEDKTSFKGGINELECAFFHAYGQDHVTVLGDTWAAAVTWYAHTYPVEWNSSGLG
                     DSWIDAKVMQSWALFGDPSLQIGGLPQ"
     gene            6536..6751
                     /locus_tag="VN377_03505"
     CDS             6536..6751
                     /locus_tag="VN377_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HWR27487.1"
                     /translation="MSLPLDVLEKAVNQKLLLLLKDGRHIEGKLLGYDEYMNLVLDEV
                     EETKDETKRRLGRIILRGSNVVSISFI"
     gene            6926..>7531
                     /locus_tag="VN377_03510"
     CDS             6926..>7531
                     /locus_tag="VN377_03510"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853936.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWR27488.1"
                     /translation="MARHVKVQRGNTLRCKGWKQEAILRMLENNLENAEKPEDLIIYG
                     GTGKAARNWKCYDAIVDALKNLGHDETLLVQSGKPVAIFRTWTTAPRVLMANSNLVPH
                     WSTWEQFRKLEQLGLTMYGQMTAGSWCYIGTQGIIQGTYETFAACARKHFKSTLTGKI
                     VLTGGLGGMGGAQPLAVTMNQGVCLAVECDEKRIDRRIKAGF"
BASE COUNT         2157 a         1725 c         1744 g         1905 t
ORIGIN      
        1 ccatatcatg ttcgaccaga cattgaaagg aattgaagaa cgggagtttt tcgacagttc
       61 caaaaccagt ttcacgttca catctgattg cagtaacgcg cgttggatgt cacgaacaat
      121 ctctttgacg agcggtgcat cgacatggac tgcgtttgcg atttttttaa tcgtacgctt
      181 cagtgaatct ccaagattat caagaaccat aggaatctac ggtgagacat agaagaaggc
      241 agattaaaag ctatcgtcat ttggtttttc ccagacatct tccacaatta tatatacaat
      301 tactgatatt gagtagtcga ggcgatgcga attgaacagt acaaataaga tcattgggat
      361 tggtgttgta agcctgttta tcattacaag tttcagcggg tgtgttcaac aaccgggacc
      421 tgggagtaac ctcattatca tcccttctgt tgatatcatc tcatcggata atccgaaata
      481 taagatggcg tcgtattatt cttctgagga aataacaatt gatgctcaaa caccacaata
      541 cagctttcct ttagatctgt caacaatcca gaatctgaac gatatcgata gtgttttttc
      601 tctgaccgtt caacaggaag aattgctgaa acggaatgga ttttttgtca aagactttgg
      661 tgttgaggac gacatcactg atccatacaa ttatctcaaa gaccatgaga tccctgtgtt
      721 cgtcacctct gatacgttgt tgcacctcta ccatatcatg ttcgaccaga cattgaaagg
      781 aattgaagaa cgggagtttt tcgacagcat ccttgacctg agcaaagcat tgttcgataa
      841 atcgattgaa gactacgaca cattcaccga gcctcatttg aaggaagcag ccagacgaaa
      901 cgtcgggttt tttggggtag cattatctct gctgcagaca ccaacaacgg gatataacaa
      961 ctcagagacc atcagaaccg tttcgttctc gataccagaa tatgttactg agaatgtcac
     1021 tactgagctt tcttttatcg aagcacatga aggattccat gagagctctc tctttcgtta
     1081 caaagaggat tactcacaat ataaccctcg tgggcattac actcaaacgg agaaactcaa
     1141 acggtacttc aaagcaatga tgtggtacgg acgaatcaca tttttactgc gtggtagcga
     1201 catcgttagc gaggtagacg caaacattgc aacgatccaa gcctgtctta tctcaacagc
     1261 gttacccgct ctcacagacg atggaagacc cttggatgaa ctctggggaa gagtctacgt
     1321 ggtcaccacg ttctttgtcg ggtcagctga cgatctcatc ccatctgagt atctcaccag
     1381 catcaacatt gtcttcggag ccaggttcga cgcgactgaa tttaccaatg agacaaaaat
     1441 ggtcaatctg cttggggcct tagcacaaca gcgaagccct cagatttatg gagggacagg
     1501 caacgctgat attcaaccac cattttcaaa agaacaactc aatgaagtcc tggaaaaaac
     1561 aaaagggatg cgactgatgg gacaacgatt catccctgat tcgtatatgt ttcagaatct
     1621 cgtgttccca accataggac aattcatggg gagtggtgag ccattcacct attggatggg
     1681 gcaacgggtc atgccacggg gtcttgatat catgaccatc cttggttcga aacgagcacg
     1741 agagctcctc aatatcgagg gtgataccgc ctatgaattc tatgaccgcc aggtagacaa
     1801 tctcaccgga cagtttgctt tattgaacac gactgagtgg aaccggaatc tgtactgggg
     1861 ctggctttac accttacaac ctctgctggg atcctttgat actcgctatc cttcgtttat
     1921 gcagaccaat gcatggagcg acaaggagct tcagacaacc cttgcctcat ggaccgagct
     1981 acgacacgat acaatcctgt acgcgaaaca atcgtacacc ccgcggatca cctcgatccc
     2041 accagaagaa acacctggta gtggctacgt cgaaccagta cctgagtttt atcaacggct
     2101 ccttgcgtta acaacgatga cacggacagg actgactgat cttcaggcat taaatgagac
     2161 agagacatat cgactgaaca atcttgaaac acttctcaga cgattactca ccatctcagc
     2221 actcgaactg aaaggaaaag gactcaacga aagcgattac gagttcatcc atacctttgg
     2281 cgaacaactc gataccatca tcaccggagt caacgaccag ggaaaggaaa ccactctcat
     2341 cgctgatgtt cacacggata ccaacacagc gtcagtcctt gaagaagcga tcggatacgt
     2401 cgacctcatc atcgttgcgt ataaaacccc caatgggaat attatcgtgg gggcaggccc
     2461 ggtgttcagt tattatgaat tcaaacaacc aatggcagat cgtttaacag atgaggcatg
     2521 gaaacaactc ttagaagcag gacaacaacc aagcagacca atctggacgg agagttttat
     2581 cgctgaatga ccctcagagg tacgtctgaa acgtggggat taatttcctt aaatccttct
     2641 cgtccacgac cacatcagcg cattgcttga tacaagcatc ctcaggatta aaagcaatac
     2701 caagaccaca gacttcaagc atcgggatat caaaacagga attccccact gcagcacagt
     2761 catccaagga aagatgaagt ttttgcacca actctttgac attcttatct ttctgcatca
     2821 gttccacatg aagaacccct tcaccggtca ggcgcccgtc agggcctacc ttgacttcat
     2881 ttgcaaacac gtagtcaatc ccaatatcag ccgcgatttt ctctgctaat atatcaagac
     2941 cagcgctgac aatcgcagtc tgtacaccat gttccttcag gaaggagata cagtcctcca
     3001 ctccgtttat aaaagggatg gagtacaata ttttttcaat ggtcgccttg ctgactgctt
     3061 ttccatcgat cttccaaagc gagacatccc gtcggatgaa ctccagataa tcgatgtccc
     3121 cccggaggta cggcatgata gaacgttcat tggtcgtccc gaaatattca tggatatatc
     3181 gccaggagct gaccgtatca agcaacactc catccatatc gaaaaaaaca agcttcaggt
     3241 tctctctttt ttttcctgcc tttgaagaag gttttttgtt cactcgataa cagggatagg
     3301 tacaggttac tctcatcaga ggcatcgcat ggctcatcac ctataataca tttattcctg
     3361 ttaatttcgt tgtcaatcct cgttctaata ctgtcctttt tataggataa tattcctcat
     3421 atggataaaa aaagaactaa acatttatta agtgtcgtgt atatttattg atggggaatt
     3481 tcctatgaaa aggttacgaa gcgcacgtat gaaaaaatca attgtttttt ttatcgcggt
     3541 atttggattt cttatcggtc cttctctcat ggcacataac acgtatctct ctgacgataa
     3601 caacgatgac aagactggtt cttctgcaga accaagtccg gtgatttatg atttgttaat
     3661 tattgctcca aaaaaatatt ctaatgcgtt acagccattg atcgttcata agaaccgtgt
     3721 tgggattaag acaaacctag tgacccttga ccaggtctat gatcaaatgt tctggcacgg
     3781 cagggataag caagagaaaa tcaagtattt tattaaaaca gctaaggaag aatgggggat
     3841 cacctatgtc atgcttgtcg gggatttcca gaccatacca attcgatatg tctataacac
     3901 tgacaacaac acaggctggg atgaaccgtg tttcatttca gagttatatt atgctgatct
     3961 ctatgacaag aaccataact tctcaacctg ggataccaac aacaacggag tctatggtga
     4021 atggtacggg gagatggcaa aagacccaga tatcgacctg taccctgatg tctttgtcgg
     4081 gcgactcgcc tgtatgaacg aagctgaagt acgggtcatg gttcgcaaga ttatcaccta
     4141 tgagacgacc gcgtatggac aaacctggtt caacaggttc gttgtcgtag cgggtgatac
     4201 ctacccggag tctgagaacc cgaactggac tgggtacgaa ggggaacaga acacgatgga
     4261 tgctatcgag aacatgtctg gtttcgaacc aataaagctg tggacgtctg acgagtcgtt
     4321 cactggacca aaagacgtca tccgtgcaat aaaccaagga tgtggtttcc tgctttttga
     4381 aggtcatgcc aatgcgtttt cctggagcac gcatccgccg aaagatgccc agacgtggat
     4441 caaaggatta aatacagtaa gcatggagcg cctgagaaac agacagatgc ttccagtgtg
     4501 tatcgtcggc ggctgtcata acaacgagtt cgatgtcaca cccgccaaca tcataaaggg
     4561 attgaaagaa gaagggttac gatatttttc tagtgggtct atcccatttg gctcgttctg
     4621 gagatatacc tggatccgag aatgctgggg atggaagctg acgcgaatca acaccggggg
     4681 atcgattgcg accatcgggt gtactgggct ggggatgtca aaggaggata aggaatcctt
     4741 cagtggtgct ggtgattatc ttgaaccgac gttcttccat gagtacggcg tgaatggaac
     4801 aaggatcctt gggaaggtgt ggggaaatac tatttctgat tatctgaatc attatccgat
     4861 tgattggaac acccctgcgg caaaggatag cgccatcgat gcgaaaaccg ttcaacaatg
     4921 ggcgctgttc ggggacccga gtctaaggat tggtggatac tctgcatcgg aattgatcag
     4981 caccctgggg taaacattta ttaagtctag catatattta taaaagggct tagaaatgaa
     5041 cacacagaaa ataagaacca tcacgacagt tggaatactc ttccttttct tcgggtcaat
     5101 catcggacct cttggtacag caacagcagc tgacacacag atggttactg aacaaaaaga
     5161 atcacatcaa gcacccctca gtatttattc gcttcttatc atcaccccga acatattcaa
     5221 aaacgaatta caaccactgg tgcgtcataa aaactcgcat ggtctgagta caatactcgt
     5281 gaccctccca gaggtctatc atcagatgta ctggtacggc cgggacgatg ctgagaaaat
     5341 aaaatatttc ataaaaacag cgatggaaga atgggggatc cgctacgttc ttctcgtagg
     5401 aggcaggagc agccagtttc tcccaacatg gtactgcccg gtacgatacg tcagcatggt
     5461 tgatgactgg gatgtcgggt atctcagcga tctgtatttc gctgacattt acgatgctga
     5521 cggaaatttt tcgagttggg atagcgatga cgatggcctg tacggtgaat ggtacatcgg
     5581 agaaacagcc caggatgtcg cttttgatct tacccccgat gtcgcggttg gcaggcttcc
     5641 ttgccgcagt gaacgggaag tcagaatcat ggtgcggaag atcatcacct atgagacaac
     5701 cgtgttgaac cagccttggt tttccaccat gctcgtcgcc gcgggggata cctaccctga
     5761 aacgagtaat gcgaattgga cggggtacga aggtgagtat tacggggatc gcgcgattga
     5821 aaacatgacg ggatttaccg cgactcgatt atacacctca gatcagactt ttttagataa
     5881 aaaaaatgtg atagaaacct tccgtaacgg atgggggttc gtgtactttg ttggccatgg
     5941 cagcccgaaa caatggggga acaatgctcc gaacggctct gagtttattc aaggtccgaa
     6001 tagcaacgac atgtggcgtt tccggaacaa agacagactc ccggtctgtg tggtcagcgg
     6061 ctgtcataac agccagttcg atgtctgtct cagacgtctt ttcaattatt ccacgcgatg
     6121 gaaacaggaa tacgtcccgg aatgctggag tgaacggatc atccgggaac catctggtgg
     6181 ttctatcagt tgcattggat gcaccggtct tgggtacaca aaagaggata aaacctcatt
     6241 caaaggaggc atcaatgaac ttgaatgcgc gtttttccat gcctatggcc aggaccatgt
     6301 cacggttctt ggcgacacct gggctgctgc ggtcacctgg tatgcacaca cctacccggt
     6361 ggaatggaat tcatctggtt taggtgactc atggatcgat gcgaaagtca tgcaatcatg
     6421 ggcactgttt ggagacccga gccttcaaat cggtggctta ccccagtaac tttcttttca
     6481 tcctccgagg tcgtaaggaa tagttttaaa aagggtgctc tccttacctt ctgccatgtc
     6541 cttaccatta gatgtccttg aaaaagcagt gaaccaaaaa cttctccttc tcttaaagga
     6601 tggcagacat attgaaggca agctgttagg atatgatgaa tacatgaacc tggtgctcga
     6661 tgaagtcgaa gaaaccaagg atgaaacgaa gcgtcgactc gggagaatta tcctcagagg
     6721 cagtaacgtc gtcagcattt cctttatata atcgaaaagc atatataggg gttattctta
     6781 ttataaattg gaaggaattg ggctggtagt tgttattttg ttagagtgca tcccatcccc
     6841 tcctagtgag gccagccgtc cttccctttt attcttctgg taacaaagtt tttgtatatc
     6901 gtattctttg tgcaaactcg agataatggc acgacacgtg aaggtccaac gggggaacac
     6961 gctccgatgt aaaggctgga aacaagaagc aatcctacgg atgctggaaa acaatcttga
     7021 gaacgcggag aaaccagagg acttgattat ctacggcggg acaggaaaag cagcgcggaa
     7081 ctggaaatgc tacgacgcga ttgtcgatgc actcaaaaac ctcggacatg acgaaacctt
     7141 actcgttcaa tcaggaaaac cggtggcaat cttccggaca tggaccacag ccccacgcgt
     7201 cctgatggca aactcaaatc ttgtccctca ttggagcacc tgggagcagt tcaggaaact
     7261 tgaacaactt ggtttgacaa tgtacggcca gatgaccgca gggtcctggt gctatatcgg
     7321 aacacaagga atcatccaag ggacctatga gacgtttgct gcctgtgcac gaaaacattt
     7381 caaaagcact cttacaggaa aaatcgtcct caccggtggt ctgggtggca tgggcggcgc
     7441 acaaccactc gcggtcacca tgaaccaagg tgtctgcctt gcagttgaat gcgacgagaa
     7501 acgcatcgat cgacggatta aagcagggtt c
//
LOCUS       DATMXJ010000129         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33937, whole genome shotgun sequence.
ACCESSION   DATMXJ010000129 DATMXJ010000000
VERSION     DATMXJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33937"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2123)
                     /locus_tag="VN377_03515"
     CDS             complement(<1..2123)
                     /locus_tag="VN377_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HWR27489.1"
                     /translation="MRELVDAFYKERSIVNHQIASYDDFLRHRLQRIVDGTIIGQEEE
                     GMKRGYIYPEIEGYKIKFGKIKVGLPQVKEADGTERPLTPMEARLRDLTYEAPIELEF
                     IPVKNEVEYEPEIVRIGELPIMVKSLRCNISQKVLEEKMDRPFSEEDYRKELQQMQED
                     PLDPGGYFISNGTERVLITVEDLAPNRVLVERSSRYGTDVEVAKVFSQREGYRALTVV
                     EKRKDGMLMVSLPTTYGQIPLIVLLRALGMENDKEIVDVICVDPKMEPYVLANIEECA
                     KEYSIATKEEAVAYLGKKFAGGQAKEYRVKRVETIMDRHLLAHLGNDPEDRLKKAIFV
                     ARMGMAVLELALGKREQDDKDHYANKRLKLAGDLMEDLFRVAFTALCKDLKYQIERIH
                     AKGKEIKIASSLRSDVLSQRIHHALATGNWVGGRAGVSQLLDRTSNLAVLSHLRRVTS
                     PLTRSQPHFEARDLHSTQWGRLCPNETPEGPNCGLVKNLALTVEISEGFGEKEVEGVL
                     RELGIKELTKHVTGARVYLNGDLIGMHPNGADLVKKLRERRRKGLLSNEINVTYDIEG
                     NDVIVNCDCGRLRRPLLVAENGKLLFNQKTLAELKEGKKKWTDFINDGVVEYIDAEEE
                     ENALIALRFENLTPDHTHMELDPMCILGIGASLVPYPEHNSSPRITMGAGMGKQSLGF
                     GASNYRIRPDTRGHLLHYPQMPLVQT"
     gene            complement(2149..2469)
                     /locus_tag="VN377_03520"
     CDS             complement(2149..2469)
                     /locus_tag="VN377_03520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HWR27490.1"
                     /translation="MAAPPKKYYDILGHKLVPEHTILSDKEKKDLLEKYHIKPDQLPR
                     ILANDPAVISAGAKPGQIVKIIRKSPTAKYATAYRLVVESDTSESFAFAEPSEEFSTA
                     SEEM"
     gene            complement(2504..2577)
                     /locus_tag="VN377_03525"
     tRNA            complement(2504..2577)
                     /locus_tag="VN377_03525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2541..2543),aa:Lys,seq:ttt)
     gene            2756..3385
                     /locus_tag="VN377_03530"
     CDS             2756..3385
                     /locus_tag="VN377_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27491.1"
                     /translation="MKINEIFFSLQGEGLLVGTPSIFVRTSGCNLRCTYCDTTYAYTK
                     GTEMTVQEILEEITKYPCKHICITGGEPLVQRQTIRLIDCLIKKNYMVCLETNGSFSI
                     QKLVGKKSLIISLDIKCPSSGSPEDMNMNNILALSKKDQLKFIIKNKEDYLYAKHILK
                     KYDPICTVFFQPVWGTDPKKLASWILHDGLDVRLSMQLHKIVWGVKRGV"
     gene            3520..>3829
                     /locus_tag="VN377_03535"
     CDS             3520..>3829
                     /locus_tag="VN377_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HWR27492.1"
                     /translation="MQPSNMAYDRAITVFSPDGRLFQVEYAREAVKRGTTTVGVKFRD
                     GAILIVDKRITSRLIEPDSIEKIFIIDDHIGCATSGLVADARSLIDRARLDAQINEIT
                     Y"
BASE COUNT          986 a          900 c          797 g         1146 t
ORIGIN      
        1 gtctgtacta atggcatttg aggataatga agcagatgac cacgagtatc tggtcggatt
       61 cggtagtttg atgcaccaaa accaagggat tgttttccca tccctgcgcc catggtaatt
      121 cttggtgatg aattatgctc cgggtaaggg acaagtgatg caccgatccc aagaatgcac
      181 atcgggtcaa gctccatgtg ggtatgatcg ggggtgagat tctcaaatcg taatgcgatc
      241 agtgcattct cttcttcttc tgcatctatg tattctacga ccccatcgtt gatgaagtct
      301 gtccattttt tcttcccttc ttttaactcg gccagggttt tttggttgaa taaaagttta
      361 ccgttttcag cgacaagcaa tggtctgcgt aatcgtccgc aatcacagtt cacgatcaca
      421 tcgtttccct cgatatcata ggtgacgtta atttcattac tcagcagtcc tttccgtcgt
      481 cgctctcgca atttcttgac gagatctgca ccgttcgggt gcatcccgat aagatccccg
      541 ttgaggtaaa cacgagcacc ggtgacatgt tttgtcaatt cctttatccc aagttcccgt
      601 aagacccctt caacttcttt ttctccgaat ccttctgata tttctaccgt cagtgcaagg
      661 ttttttacca acccgcagtt cggtccttct ggtgtttcat tggggcagag acgtccccat
      721 tgtgtcgaat gtaaatctcg ggcttcgaaa tgtggttgtg atctggttaa cggcgaggtc
      781 acccgtctaa gatgactgag aaccgcaaga ttactggttc ggtcaagaag ctgggagact
      841 ccggcacggc cacccaccca gttccctgta gcaagtgcat gatggatacg ttggctaagg
      901 acatcggagc gtaatgagga tgcgattttg atttcttttc ctttcgcatg gatccgttca
      961 atctgatatt ttaaatcctt gcagagtgcg gtaaaggcga cccggaacag atcttccata
     1021 agatccccgg caagtttcag ccgtttgttt gcgtaatggt ccttgtcatc ctgctctcgt
     1081 ttccccagcg cgagctctaa gactgccatc cccatgcgag cgacgaaaat agcttttttc
     1141 aaacgatcct ccgggtcgtt gcccagatgc gcaagaaggt ggcggtccat gatggtttca
     1201 acccgtttca cccggtactc ttttgcctgc ccaccagcaa atttcttccc aaggtacgcc
     1261 acggcctctt cctttgtcgc gatactgtat tccttggcgc attcctcgat gtttgctagc
     1321 acgtacggtt ccatctttgg atccacacag atgacatcga cgatttcttt atcgttctcc
     1381 atcccaagtg ccctaagtaa aacaatgagt ggaatttgac cataggtcgt tggtagactc
     1441 accatgagca taccgtcctt tcgtttttca accacggtta atgctcggta tccttcccgt
     1501 tgggagaaca cttttgctac ttcaacatca gtcccgtatc ggcttgaccg ctcgacaaga
     1561 actcggtttg gagcgaggtc ctcaacggta attaacacac gttctgttcc attactgata
     1621 aaatacccgc cggggtccag gggatcttcc tgcatttgct gcagttcctt tcgataatcc
     1681 tcttccgaga aggggcggtc cattttttct tccaaaacct tttgagaaat gttacatctc
     1741 agggatttta ccatgatggg tagttcacca atacgtacta tttcaggctc gtactccacc
     1801 tcgtttttca cagggataaa ctcaagctcg atgggcgctt cgtaggttag gtcccgtaac
     1861 cgtgcttcca ttggtgtcag tggtcgttcc gtcccatctg cctctttgac ctgtggaaga
     1921 ccgaccttga ttttcccaaa ttttatctta tatccttcta tctcagggta gatgtaccct
     1981 cgtttcattc cttcttcttc ctggccgatg attgttccat cgacaattcg ctgcaatcga
     2041 tgtcgtaaga aatcatcata ggaagcgatc tgatggttga caatacttcg ctctttataa
     2101 aacgcatcaa cgagttctct cataaatact cctcatattc cccttttttt acatctcttc
     2161 acttgccgtg ctgaactcct cagaaggttc agcgaaagca aaactctcag aggtatcact
     2221 ctcaacaact aaccgatagg cggtcgcata tttcgctgtc ggacttttac gtataatttt
     2281 cacgatctga ccaggtttcg ctcctgcgga aatcaccgct gggtcgttag caagaatacg
     2341 tggaagctga tctggtttga tatggtattt ctccaacagg tcctttttct ctttatcaga
     2401 taatatagta tgttctggaa ccagcttatg tcctaagatg tcgtaatatt tttttggcgg
     2461 ggctgcgata ctgcattcct cctacgttcc tcgtgaggaa aggcgggcct gaagggattc
     2521 gaacccttgg ccacctggtt aaaagccaga tgctctacct agctgagcta caggcccaaa
     2581 gatgggccgc attgcattga ctttatatag tatacgctat atcaagtaac attggaacgg
     2641 cttacccgta atacaatttc ccctataaat atttttactt cctatttctt tccctcattc
     2701 cttctttttt ttcttatgaa aagattttaa ttcacatcca aaatacatca tgtaaatgaa
     2761 aataaatgag atattctttt cactacaagg cgaaggtctc cttgttggga ctccaagcat
     2821 ttttgttaga acctctggat gtaacctcag atgcacctat tgcgatacga cctatgcgta
     2881 caccaaagga acggaaatga cggtacaaga gatcctggaa gagataacga aatatccatg
     2941 taaacatatc tgtattacag gtggggagcc actcgttcag agacaaacca taagacttat
     3001 cgactgtctc attaaaaaaa actatatggt atgcctagaa accaatggta gtttcagtat
     3061 tcagaaactt gttggaaaga aatctcttat catttctctt gacataaaat gtccttcctc
     3121 aggctcccct gaagacatga atatgaacaa tatcctcgct ctttcaaaaa aagatcaact
     3181 caaattcatt atcaagaata aagaagatta cctttacgca aaacatattc tcaaaaaata
     3241 cgatccgatc tgcactgttt ttttccagcc ggtttgggga acggacccaa agaagcttgc
     3301 gtcatggatt ctccatgatg gattagacgt acgattatca atgcagcttc ataaaatcgt
     3361 ctggggtgta aaaagaggag tctgaccgag tcatgtttgt atcgtttttt tctagagaag
     3421 aaacgtaaac acaaactata tacatgaaaa gtgcattcat ggttgaaatt aagagaaatt
     3481 agtgagaaaa acagaagaaa agatgaggtg aaataaaaaa tgcaaccatc aaatatggca
     3541 tatgatagag cgattaccgt cttttcccca gatggacgct tatttcaggt agagtacgcc
     3601 cgggaagcgg taaaacgtgg aaccacaacc gtgggagtga aatttcgaga tggtgctatt
     3661 ttaattgttg ataagcgtat tacctctcga ctgattgagc ctgattcaat tgagaagata
     3721 ttcattattg atgatcacat cggttgtgca acctcaggtc ttgttgccga tgctcgttcc
     3781 ttgattgatc gcgctcgtct agacgcgcag attaatgaaa taacataca
//
LOCUS       DATMXJ010000130         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7816, whole genome shotgun sequence.
ACCESSION   DATMXJ010000130 DATMXJ010000000
VERSION     DATMXJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7816"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VN377_03540"
     CDS             complement(<1..731)
                     /locus_tag="VN377_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HWR27493.1"
                     /translation="MSKDFSVHVEHITRVEGHGNIILDVKNGTIEKLQWSVVEAPRFF
                     EAMLRGQHYNELRPITSRICGICSIGHSLASLKATEDALGVKITNQTATLRRLAIHAE
                     NMQSHILHIGYLVAPDLLGAGSVFSLLGKHTDTVLKIVKLHRLANEMSDLICGRTIHP
                     IRLIVGGFSLQPTEKQLIQLKKRLLEAQELTKELAPIILSVAGNLPSFTRETEYIGLT
                     SDAEYSLYDGDIASTDTGRHPKYRD"
     gene            complement(724..1488)
                     /locus_tag="VN377_03545"
     CDS             complement(724..1488)
                     /locus_tag="VN377_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27494.1"
                     /translation="MKQKPRVAVFDFADCEGCELEIADLEEEVLELLEIVDIVSFREV
                     MKEHSDLYDIAIVEGSIMRPMDEKRLRSIRSRAKILIALGACATMGGVNKIRNQWMPD
                     EVKKEVYGTANLKGNELFDVFQTKALNEVVPVDYYIYGCPIDRDEFKSVITAIALGKK
                     PDIPRYPVCVECKKNENICQFELGSFCLGPITRAGCKAICPTHNSPCEGCRGLIKKAE
                     TECVKEVLERYKLSYEDIRRRCTMYNYGRKECSYDE"
     gene            complement(1485..>2596)
                     /locus_tag="VN377_03550"
     CDS             complement(1485..>2596)
                     /locus_tag="VN377_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWR27495.1"
                     /translation="CMLKDFTKVGSGEVFRDDVKERYRHRFFRKGNYLPERYGFVACV
                     GCGRCGSACLPDIADPCNLINELAHFTSEDDTGKYFIREENEVLEKGIIHVPRSATIK
                     KITPFNELDSLFEIELDDKKPLGHKPGQFVEVSVFGYGEAPFGISTPPGNTPIFEIMV
                     RQVGNVTKKLCSLQPGDKIGIRGPLGNGFDVTSFEGKTLLFTSGGTGMVPMRSLINHV
                     LDPTQRNKFKDIIILYGAKRPKEITFMDDVDHWKKIHNVQCELTVDRCEPSECWGGCT
                     GLITTLFPKIKADKLDSKNTIAVVIGPPIMYKFVIKCLQTLGVPDDNIYVSLERRMKC
                     GVAKCGHCQINGIYVCKEGPVFNYGRLKQLPEAFE"
BASE COUNT          668 a          603 c          528 g          797 t
ORIGIN      
        1 taatcccgat atttgggatg ccttcctgta tctgttgagg cgatgtctcc atcgtacaaa
       61 gaatattccg catcagaggt taaaccaata tattctgttt cccgggtgaa tgaaggcaga
      121 ttaccggcaa cgcttagtat gatgggagca agttcctttg tgagttcttg tgcttcgagc
      181 agtctttttt ttaattggat caattgtttt tcagtaggtt gcagtgaaaa accacctacg
      241 attagtcgaa ttggatgtat ggttcgtcca caaattaaat ctgacatttc atttgccaga
      301 cgatgcagtt taacaatttt cagaacagtg tccgtgtgtt ttccaagtaa agaaaacacg
      361 ctaccagcac cgaggaggtc aggtgccacg aggtacccga tgtgaagaat atggctctgc
      421 atgttctctg catgaatcgc taatcgacgt agcgtcgcgg tttggttcgt aatttttact
      481 cctaatgcgt cctcggtcgc cttgagtgac gcaagggaat gaccaatcga acaaatacca
      541 cagatccgtg acgtgattgg gcgcaactcg ttataatgtt gcccgcgtaa catggcttca
      601 aagaatcttg gcgcctctac aacgctccat tgaagtttct caatcgtacc gttcttgaca
      661 tcgaggatga tatttccatg gccttcaact cgagtgatat gttcaacatg aaccgaaaaa
      721 tctttactca tcgtaactac actcctttcg tccataatta tacattgtac atcgtcgtcg
      781 tatatcctca taggaaagct tgtatcgttc caatacttct tttacgcatt ccgtctctgc
      841 tttcttaatc agacctctac agccctcaca agggctgtta tgggtcggac atatcgcttt
      901 acacccagct ctggtgattg gtccaagaca gaacgaacca agctcaaact gacagatatt
      961 ctcgtttttc ttacattcca cacaaacagg ataacggggg atatcaggtt tcttcccaag
     1021 agctatggct gtgataaccg atttgaattc atctcgatct atcggacagc catagatata
     1081 ataatcaact ggaacaacct cattgagtgc ttttgtttgg aacacatcaa agagttcgtt
     1141 tcctttcaga ttcgcggtgc catagacctc tttttttact tcatcgggca tccattgatt
     1201 ccgaatttta ttgactccgc ccatggtcgc acaagcacct agtgcgatta gaatttttgc
     1261 gcgactccga atgctacgta atcgtttctc atccatcggt ctcatgatgg agccttcgac
     1321 gatcgcgata tcatataaat ctgaatgttc tttcatcacc tcacggaatg agacgatatc
     1381 tacgatttca aggagttcta gtacttcttc ttcaaggtca gcgatctcta gctcacatcc
     1441 ttcgcaatcc gcaaaatcaa agacagcgac ccgtggtttt tgtttcattc aaacgcctcc
     1501 ggtagttgtt taagcctgcc gtaattgaat actgggcctt ccttgcaaac gtagatccca
     1561 tttatctggc aatgaccgca ttttgcgaca ccacatttca tgcgtcgctc aagagacacg
     1621 tagatattat cgtcagggac accaagggtt tgcaagcatt taatgacaaa tttatacata
     1681 atcggtggtc caataactac cgctattgta ttttttgaat caagtttatc tgcctttatt
     1741 ttcgggaata atgtggtgat cagaccggtg catcctcccc agcattcgct tggctcacat
     1801 cgatcgacgg ttagttcaca ttgaacgttg tgaatttttt tccagtggtc aacgtcatcc
     1861 ataaaggtta tctcctttgg acgtttcgca ccatacaata tgatgatgtc cttgaattta
     1921 tttctttgtg ttggatccag gacatgatta attaacgaac gcatcggcac cataccggtt
     1981 ccaccagaag tgaaaaggag tgtttttcct tcgaatgatg tcacatcgaa tccgtttccc
     2041 aatggtccgc gaataccgat tttatcacca ggttgtaacg agcataattt ttttgtcaca
     2101 tttccgactt gtcggaccat aatttcaaaa attggagtat tccccggtgg tgtggagatg
     2161 ccaaagggtg cttcaccata cccgaaaacg gatacctcga caaactgtcc gggtttatgt
     2221 cctaatggtt ttttatcatc tagttcgatt tcaaagaggc tatcaagttc attaaagggt
     2281 gttatttttt taatcgttgc actgcgtgga acatgaataa tccctttttc caacacctcg
     2341 ttttcttccc gtatgaaata ttttcctgta tcatcctctg aggtaaaatg tgcaagttca
     2401 ttgataaggt tgcagggatc agcgatatct ggtaagcagg cgctgccgca tctgccacat
     2461 ccgacgcagg cgacaaagcc atagcgctct ggtaggtaat tgccctttcg gaagaaccgg
     2521 tgacggtatc gttcttttac gtcatcgcgg aagacctcac cgcttccaac tttagtgaaa
     2581 tccttaagca tgcaac
//
LOCUS       DATMXJ010000131         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33948, whole genome shotgun sequence.
ACCESSION   DATMXJ010000131 DATMXJ010000000
VERSION     DATMXJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33948"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1340
                     /locus_tag="VN377_03555"
     CDS             93..1340
                     /locus_tag="VN377_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWR27496.1"
                     /translation="MVVNYDRLFSTRSKNLKSSEIRELLKLTQSPGFISLAGGLPNPD
                     AFPIDVIHECIEKVFKTDIHNALQYGTTEGLPQLRNVLAQRMRERKGIDCELHDILIT
                     SGAQQALSFVAFNLLDPGDTYITSAPTYLGALQAFHAYQGNCECIPMNDEGIDIDSLR
                     RNLKRLHRTGITPKFLYAVATFQNPSGESISLEHRKELLEIASEYDFVIIEDDPYGEL
                     VFEGEEIPSIKSLDTKGRVIYIGTFSKVLAPGFRLAWIIGSEEIINRFSLTKQSADLC
                     SNMFVQYVAYEYIKGGYLDIQIKHIKLLYRHKRDVMIKALKEYFPKEAKWTVPGGGMF
                     LWVTLPKNVNTRLMFKRAVAKQVAYVVGDAFYPDSSNYNSMRLNFSYSDDDVIREGIK
                     RLAEVIKEEMATPYDRDEYVGGV"
     gene            1469..1687
                     /locus_tag="VN377_03560"
     CDS             1469..1687
                     /locus_tag="VN377_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HWR27497.1"
                     /translation="MGNIRQTHIKSTAIDLVKKYPELFTGDFQNNKEKVDLLVTVSSK
                     LLRNRIAGYITRYVVSRSKSKPSRPISE"
     gene            1790..2434
                     /gene="ribB"
                     /locus_tag="VN377_03565"
     CDS             1790..2434
                     /gene="ribB"
                     /locus_tag="VN377_03565"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWR27498.1"
                     /translation="MYDNESRESETDLVIASQFVTPESIKRMRKDGGGLIFLMISHQA
                     AQTLQLPFLSDLFSEVSPDYPVLKALVPNDIPYDTKSSFSLYINHRKTFTGITDNDRS
                     LTMKKFAELVKRTKDTDSNTAVKELGKEFRSPGHIPICIASKKLLNERKGHTELVISL
                     LEMAGLIPVGSGCEIMGEHGKALPKEDAKKYAQKNNLVFLEGNDIIKAWKQWSK"
     gene            2422..2883
                     /locus_tag="VN377_03570"
     CDS             2422..2883
                     /locus_tag="VN377_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWR27499.1"
                     /translation="MVKVMATGTFDLLHMGHIYYLKEAKKLGDRLVVVVATDATVRKL
                     KHDPITPQEIRVNLIKELKVVDEAYLGHEQDMYAIVEEIKPDIIALGFDQIHDAKTIQ
                     NELKKRKLNIKVVRLSKYQGESDLEGTRRIIQKVIEAHEFQKKMQRIEGEP"
     gene            2880..>2980
                     /locus_tag="VN377_03575"
     CDS             2880..>2980
                     /locus_tag="VN377_03575"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605261.1"
                     /note="catalyzes the formation of riboflavin and
                     4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine from
                     6,7-dimethyl-8-(1-D-ribityl)lumazine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWR27500.1"
                     /translation="MKRIGIADTTFARYNMAHAAINELQSLATGFTI"
BASE COUNT          935 a          671 c          652 g          722 t
ORIGIN      
        1 tataaacaac cgtcgatttc aaaaaaaatg gtagcgagtg taaataccaa attttaaaat
       61 aagggaactg attacaggca cggggcattt ttatggtagt taattacgac cgtttattct
      121 cgacacgatc aaaaaacctg aaatcctcgg agatacgtga gttattgaaa ctcacgcaaa
      181 gcccagggtt tatctctcta gcggggggat taccgaatcc tgatgcattc cctatcgatg
      241 tcatccatga atgcatcgag aaggtcttta aaaccgatat ccataacgcc cttcaatacg
      301 gaacaaccga gggtttaccg cagcttcgga acgttctagc gcagaggatg agagaacgaa
      361 aggggattga ttgtgagctc catgacattc ttatcacgag tggtgcccag caggcgctgt
      421 cgtttgtcgc gtttaactta ttagacccgg gggataccta tatcacgagc gcaccgacct
      481 atcttggtgc cctgcaggcg tttcatgcgt atcaggggaa ctgcgaatgc atcccgatga
      541 atgacgaagg aattgacatc gattccttgc ggagaaacct gaagcgtctt caccgaaccg
      601 gtattacccc gaagtttctg tatgcggtcg cgacgttcca gaacccgtct ggagaaagca
      661 tctcgttgga acatcgaaaa gagctcctcg agatcgcctc ggagtatgat tttgtcatca
      721 ttgaggatga tccctatggt gaactggtct ttgaaggaga agagatcccg tcgattaaat
      781 cattggacac gaagggtcgt gtcatctata ttgggacgtt ttctaaggtg ctggcacctg
      841 gtttccgcct cgcttggatc atcggctctg aggagatcat caaccgattc tccctcacaa
      901 aacaatccgc tgatctttgt tccaacatgt tcgttcaata tgttgcctat gaatacatca
      961 aaggcggtta tcttgatatt cagattaaac atatcaagct gctttatcgg cataagcggg
     1021 atgtgatgat taaagcgttg aaggaatatt tcccgaaaga ggcgaaatgg acagtcccag
     1081 gtggcgggat gttcctgtgg gtcaccttgc cgaaaaatgt gaacacgcgt ctgatgttta
     1141 aacgagcggt ggcaaagcaa gtcgcgtatg tggttggcga tgcgttctat ccggatagca
     1201 gcaattacaa ctcgatgaga ttgaattttt cgtattctga cgatgatgtg attcgagaag
     1261 ggattaaacg tctcgcggaa gtcataaaag aagaaatggc tactccctat gatagagatg
     1321 agtacgtggg aggagtataa ccttattttt cccatttttc ttttttttaa tatatcgtag
     1381 aatgtgaaaa gaaacaagaa ttctccctcg cactaacttt aaataacaaa acactattcc
     1441 ggaagcctat atcttaaggg tgattcatct gggcaatata cgacagacac atatcaagag
     1501 cactgcaatt gatttagtaa agaagtatcc agaactattt actggtgatt ttcagaataa
     1561 taaagaaaag gttgatttac ttgtgaccgt gagtagtaaa cttctaagaa atagaattgc
     1621 tgggtacatt actcgatacg tggtttctcg gagtaagtca aaaccaagtc gacccatttc
     1681 tgagtaacat cccaatattt attttatctt gtgaaatgtt cctcacaggt gaattcttat
     1741 ttccgtacag aacgctatcg aatccctcca aaagggacaa tttatactca tctatgataa
     1801 cgaatcaaga gaaagcgaaa cagacctcgt catcgcctcc cagttcgtca cccccgaatc
     1861 aatcaaacgc atgcgaaaag acggtggagg actcatcttc ctcatgatat ctcaccaggc
     1921 agcacagaca ctacaactcc cctttctctc agacctattt tctgaggtga gccccgatta
     1981 cccagtcctc aaagcgctcg tcccaaatga catcccctac gacacgaaat cctctttctc
     2041 cctgtacata aatcaccgga aaacatttac cgggatcacg gacaatgacc gaagtctgac
     2101 catgaaaaaa ttcgcagaac ttgtgaagag aacaaaagat acagacagca acactgcggt
     2161 aaaagaactc ggaaaagaat tccgttcccc cggacatatc cccatctgca tcgcctcaaa
     2221 aaaactcctc aacgagcgaa aaggtcatac ggagctagtg atttcattac ttgaaatggc
     2281 aggacttatc cctgtcggaa gcggttgtga gatcatggga gaacacggaa aagcattacc
     2341 aaaagaagat gcaaagaaat acgctcaaaa aaacaacctt gtgttccttg aaggaaacga
     2401 tattatcaag gcgtggaaac aatggtcaaa gtaatggcaa ccgggacttt tgatttacta
     2461 cacatgggac atatctacta tctgaaagaa gcaaaaaaac taggggacag gcttgtggtt
     2521 gttgtcgcga ctgatgcaac agtacgcaaa ctcaaacatg acccaatcac accgcaagag
     2581 atacgagtca atctcatcaa agaactcaaa gtcgtcgatg aagcatacct tggtcatgag
     2641 caggatatgt atgcaatcgt cgaagaaata aaaccggaca tcatcgccct tggttttgac
     2701 cagatccacg atgcaaaaac aatccagaac gagctgaaaa aaaggaaact caacataaag
     2761 gtcgtccgac tctcgaaata ccaaggggaa agcgacctgg aaggcacccg tcggataatc
     2821 caaaaagtca tcgaagccca cgagttccaa aagaagatgc aacgtatcga aggagaacca
     2881 tgaaacgaat cggcatcgca gacaccacct ttgcccgcta caatatggca cacgcggcaa
     2941 tcaacgaact gcaatccctc gctacagggt ttacgatcat
//
LOCUS       DATMXJ010000132         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_2639, whole genome shotgun sequence.
ACCESSION   DATMXJ010000132 DATMXJ010000000
VERSION     DATMXJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VN377_03580"
     CDS             complement(<1..680)
                     /locus_tag="VN377_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HWR27501.1"
                     /translation="MTQEYWDRKIETMPLTELKQVQLKRLQKLVKYVNTSNQFYHKKL
                     SEAKVKPESIKTLKDIEKLPFLTKQDLRSYYPFGLVCTPLDDIIEVHASSGTTGKPVV
                     GPYTRNDVDLWGEMMARSLYANGLRKNDVLQNAYGYGLFTGAHGFEKGAEKIGAMVIT
                     IGSGNTERQIDIMRDFGSTALACTPSYSLHIAEVAEEKGLNPAEDFKLRLGAFGAESW
                     SEEMRKNI"
     gene            841..972
                     /locus_tag="VN377_03585"
     CDS             841..972
                     /locus_tag="VN377_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27502.1"
                     /translation="MINIPDHIIIGLPPSFLLTILISIIRKQDNEEEILMNKSFGNI"
     gene            1015..2172
                     /locus_tag="VN377_03590"
     CDS             1015..2172
                     /locus_tag="VN377_03590"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWR27503.1"
                     /translation="MNVLILGAGMMGRAIAYDLSRFSSFDDIILGEKDSRTQTTAKRF
                     LQGTRVEVKTLDAERPSEIKKRVQKGDVVISALPYKYNYKLAKLAIQAKTHFIDLGGN
                     NTIVDNERRLFSQAKKNDVTIIPDSGLAPGLVSIITRDIVDHLDTVDFVKLRVGGLPR
                     YPKPPLNYQIVFSPYGLINEYVEDALILDQGKIRNKKSMTEVESLEFPKPFGTMEAFL
                     TSGGCSTLPYTYRRTINYLDYKTIRYPGHCEKIKAILDLGFADEKKINCVGQMIAPRD
                     IFASLLMKNVPTTGEDVVLLKVISEGIIQGEKRSREYLLIDYYDRKHNITAMMRTTGY
                     PAAITAQMIENGTIQERGVFCPEEIIPPTVFFNELKRRDILLSISDRSVSK"
     gene            2169..2759
                     /locus_tag="VN377_03595"
     CDS             2169..2759
                     /locus_tag="VN377_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27504.1"
                     /translation="MKKKEFLDAKENALKSLNDACDQGKVDTGALPILDLINKIGGFY
                     TSSSCAGRIVLLQIPQIGDKRGATFLGIWHRTIEPDELRTAATKATTGFLWLLAQAPI
                     LHIGAETLQLADKMVKTAISCGFKNSAVKSIEKKIIIEINSTERLDAPIGRDGCLFCD
                     EKQLSLLVEISNEVIERSREKMGRLTKKLGNIAEFL"
BASE COUNT          895 a          617 c          588 g          765 t
ORIGIN      
        1 tcgatattct tccgcatctc ttcactccag gattctgctc cgaacgcacc taatcgaagc
       61 ttgaaatcct ccgcagggtt caatcctttc tcttcagcga cctctgcaat atgcaaggaa
      121 tatgaaggtg tacacgcaag cgcagtacta ccaaaatccc tcatgatatc tatctggcgc
      181 tcagtgttcc ccgagccaat cgtaatcacc attgcaccaa ttttctcagc acccttctca
      241 aagccgtgcg ctccggtgaa caacccgtat ccataggcat tctgaagcac atcgtttttt
      301 cgaaggccgt tcgcatataa ggaccgtgcc atcatctccc cccagagatc cacatcattt
      361 cttgtataag gacctacgac cggttttccg gttgtccctg atgatgcgtg tacctctatg
      421 atatcatcaa gaggggtaca gaccaaacca aaaggataat acgagcgtaa atcttgtttt
      481 gttaaaaaag ggagtttctc aatatccttt aatgttttta tcgactcggg tttcaccttg
      541 gcttctgaaa gtttcttatg ataaaattga ttcgaggtat tgacatattt tactaatttt
      601 tgcaatcgct tcaattgtac ttgttttaat tcagttaatg gcattgtttc tatcttgcgg
      661 tcccaatatt cctgtgtcat aaaaaacact ccaattttgg caggaagaat acagagttgc
      721 tctataagag tttctgaatg aaaaataaat aaagatacaa aataagagac ttggtttaca
      781 actctttaaa aatcaattga acaatctatt ttggaggaga cactcctttc tgggaattac
      841 atgatcaata tacctgacca tatcattatt ggccttccac cgtctttcct gttgacgatt
      901 ctcattagca tcatacggaa gcaggataac gaggaagaaa tcctcatgaa caagagcttt
      961 ggaaacattt aacaggaaga tatcaatcct gcatctgata atgggtcggt cgatatgaac
     1021 gtactaatac tcggagcagg tatgatgggt cgtgcgattg cttacgatct gtcacgcttc
     1081 tcaagtttcg atgacatcat tcttggagaa aaagacagta gaacacaaac aacagcgaaa
     1141 cgatttctcc aaggaactcg ggttgaagta aaaacgcttg acgccgagag accatctgag
     1201 attaaaaaac gagtacaaaa aggggatgtc gttatctctg cgctccccta taaatataat
     1261 tacaagttgg ccaagctagc gatacaggca aaaacacatt ttatcgacct tggtgggaac
     1321 aacacgattg ttgataatga gcgacgtctt ttttcgcaag cgaaaaaaaa cgatgtgacc
     1381 attatccctg attcagggtt ggcacctggt cttgtatcca tcatcacccg ggacatcgtc
     1441 gatcatctgg atacagtcga cttcgttaaa ctccgcgtgg gaggattacc acgttatccg
     1501 aaacccccct tgaattatca gatcgtgttt tcaccctatg gccttatcaa tgaatatgta
     1561 gaagacgcct tgattctcga ccaggggaag atccgtaata agaaatctat gaccgaagtt
     1621 gaatcattgg aatttccaaa accgtttgga acaatggaag cgttccttac ctcaggtgga
     1681 tgctcaacct taccatatac atatagacgt acaatcaact atcttgatta taaaacgata
     1741 cgatacccag gacattgtga aaaaatcaag gcgatactag atcttggatt cgctgatgaa
     1801 aaaaagatca attgcgttgg tcagatgatt gcccctcggg atatcttcgc gtcattactt
     1861 atgaaaaacg ttccaactac aggagaagac gtcgtgctgt tgaaagtgat aagtgaaggg
     1921 ataatacagg gggagaagcg atccagggaa tacctcttaa tagattacta tgacaggaaa
     1981 cataacatca ctgcaatgat gcggacgacc ggctatcctg cggccatcac cgcccagatg
     2041 atcgagaatg gaactatcca ggagcggggt gtgttctgtc cagaggaaat cattccaccg
     2101 actgtttttt tcaacgaact aaagagaaga gatatcctgc tttcgatttc tgatagaagc
     2161 gtttcgaaat gaagaaaaaa gagttcctag atgcgaaaga aaacgcgttg aaatccctga
     2221 atgatgcgtg cgaccaagga aaggtcgaca ctggtgcgct cccaatcctt gatctaataa
     2281 ataagatcgg aggattctac acctcaagta gttgtgcagg ccgcattgtt ttactacaga
     2341 taccacagat tggtgacaaa cgcggtgcga cattccttgg gatttggcat cgaaccatag
     2401 aaccagatga gctgcgaact gccgcgacaa aggcgacaac tggttttctc tggttactag
     2461 cccaagcacc gatcctccac ataggtgctg aaaccctcca acttgcggac aaaatggtaa
     2521 aaaccgcgat atcctgtgga ttcaaaaact cagcagtaaa atcaatagaa aaaaaaatca
     2581 tcattgaaat caacagtacc gagcgtcttg atgcccctat agggagagat ggctgtcttt
     2641 tttgcgacga aaaacaactc tctttactgg tcgaaatctc aaatgaggtc atagaacgat
     2701 ctcgggaaaa aatgggccga cttaccaaaa aactaggaaa tattgccgaa tttctatgaa
     2761 aatcaacaaa agatttatat attatgttga tatattagtt gttggggaat aaagtatgaa
     2821 cacaaaaaaa atcttaggca tctttatatg catgctggta atgac
//
LOCUS       DATMXJ010000133         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_65577, whole genome shotgun sequence.
ACCESSION   DATMXJ010000133 DATMXJ010000000
VERSION     DATMXJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65577"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VN377_03600"
     CDS             <1..263
                     /locus_tag="VN377_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HWR27505.1"
                     /translation="IRTKETIKEGPLGGKKFVFTGGLASMSRPDASDLVMKNGGIVAS
                     AIGKDIDYVVVGTDPGSKYEKAKKLRLTIIDENEFKKIVGAL"
     gene            266..463
                     /locus_tag="VN377_03605"
     CDS             266..463
                     /locus_tag="VN377_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27506.1"
                     /translation="MVECKIQKNKLVCNCSYSCSRKGICCECLSYHRNRDELPACFFP
                     DPIERTYNRSRENFIQTCQGK"
     gene            646..879
                     /locus_tag="VN377_03610"
     CDS             646..879
                     /locus_tag="VN377_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR27507.1"
                     /translation="MAIGFVLISAAPAHEHEVYNKLSKVPQIIELHPLFGEYDLIAKI
                     EADDFESLGDIVVNKIRTIEGVIDTKTLTGTKF"
     gene            894..1208
                     /locus_tag="VN377_03615"
     CDS             894..1208
                     /locus_tag="VN377_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27508.1"
                     /translation="MEGLVQYFLSMMTLVFAIASVIAGIFTAYFGSGKSRAVGAILII
                     IGLIVGVIFLWGANLLGFMGTPMDLLNFSGTIVNGIIAVIGAVVGALIALGIFLLAIM
                     KA"
     gene            complement(1209..1820)
                     /gene="pdxT"
                     /locus_tag="VN377_03620"
     CDS             complement(1209..1820)
                     /gene="pdxT"
                     /locus_tag="VN377_03620"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWR27509.1"
                     /translation="MGTRLVEIVTVGVLSLQGAVSEHLISMNRVFQEGKKKGHSVVVR
                     QPRDIDACDALIIPGGESTTISRILVNSGMHDIIRERMNHNDIALMGTCAGCVLLARE
                     LTDIKADVKLLEAMDMQVERNAYGRQKESFERSLQIKGFTRSYHAVFIRAPLITKIWG
                     DCEVLATAGKEIVMARQKNYLALSFHPELTDDLRIHRFFLEML"
     gene            complement(1802..>2455)
                     /locus_tag="VN377_03625"
     CDS             complement(1802..>2455)
                     /locus_tag="VN377_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HWR27510.1"
                     /translation="ADPFYHIDKRAFTIPFVCGCRNLGEATRRIWEGAAMIRTKGEAG
                     TGNIIEAVRHQRMVTSMISTLKTMPSAKIQKVAEGYCQSYVISYKTLTDQNVDEKTIV
                     FGSFAYSTLKKELYEILKEIKQLQRLPVVNFAAGGIATPADAAIMMQLGSDGVFVGSG
                     IFKSKNPEKRAKAIVEAVAHFDEPETIADVSKNLGDAMLGIEISSIPKEQLLSERGW"
BASE COUNT          696 a          511 c          560 g          688 t
ORIGIN      
        1 ggatacgaac caaagaaacg ataaaggaag gacctctcgg cggtaaaaaa ttcgtgttca
       61 ccgggggact tgcgtcaatg tccaggccag atgcctcaga ccttgttatg aaaaacggtg
      121 gcatcgttgc ctccgcgata ggaaaagaca ttgattatgt cgtcgtcggt accgaccctg
      181 gctcaaaata cgaaaaagca aaaaaactga ggttaaccat cattgatgaa aacgagttta
      241 aaaaaatagt tggagcattg tgaccatggt ggaatgtaag atacagaaaa acaagcttgt
      301 ttgtaattgt agttattctt gtagcaggaa agggatctgt tgtgagtgcc tcagctacca
      361 taggaacaga gatgagctgc ctgcgtgttt tttcccagat cctattgaac ggacctataa
      421 tcgaagtagg gagaatttta ttcagacatg ccaagggaaa taaaagctaa aaacaacatg
      481 tcgtccttga acaaataccc gtagagattt gatggagtca taagtgatca aaaacaaaga
      541 aaatgaaaaa cgtaaaacac cttttttcag atagaaggga aatagtacat aacgtaagac
      601 ttatattcaa tgacattata acggtgagcg aggaaggtaa aatatatggc aattgggttt
      661 gtcctgataa gtgccgctcc cgcacatgaa catgaggtct acaacaaact ctcaaaagta
      721 ccacagatta tagaactcca tccgttattt ggggaatatg atctcatcgc aaaaatagaa
      781 gcagatgact tcgaaagcct cggagatatt gtggtaaaca aaatcagaac aatcgaaggt
      841 gtaatcgata cgaaaacact aacgggaacg aagttttaag gagtgaaaaa aatatggaag
      901 gattagtaca atattttcta agcatgatga cgctggtgtt tgcaatagca agtgtaatcg
      961 caggaatttt taccgcttat ttcggctctg gaaaaagcag agcagttgga gcgatcttaa
     1021 tcattatagg tcttatcgtt ggagtcatct tcctctgggg tgcgaacttg ttaggtttca
     1081 tgggaacccc aatggacctg ttgaactttt caggaacgat agtcaacggg attatcgcgg
     1141 tcatcggtgc tgttgttgga gcgctgattg ctcttggaat cttcctgctt gcgatcatga
     1201 aggcatagct acagcatctc taaaaaaaat cgatgtatcc tgagatcatc ggtaagctca
     1261 gggtgaaacg ataacgccag gtagttcttc tgcctggcca tgactatttc ttttccagcg
     1321 gttgcgagta cttcgcagtc gccccatatt tttgtgataa gaggcgctcg gatgaacacc
     1381 gcatgatacg agcgggtaaa tccctttatc tgaagtgatc gttcaaacga ttctttttgt
     1441 ctgccgtatg cattgcgttc tacctgcatg tccattgctt ctaagagttt gacatccgcc
     1501 ttgatatcag ttaattctct ggcaagtaaa acgcatcctg cacaggtccc cataagcgcg
     1561 atgtcattgt ggttcattcg ttcacggatg atatcatgca tccctgaatt gactaaaatc
     1621 cgagagatcg tcgtactctc cccacccggg ataattaacg catcgcacgc atcgatatca
     1681 cgtggttggc gtaccactac tgaatgaccc ttcttttttc cttcttgaaa aacgcggttc
     1741 attgatatga gatgttcgga aaccgctcct tgaagggata aaacaccgac tgtgactatt
     1801 tctaccagcc tcgttccgat agaagttgtt cttttggaat cgagctaatt tcgatcccaa
     1861 gcatggcatc gccaaggttt ttactgacgt cagcaatcgt ttctggttca tcaaaatgag
     1921 cgacagcctc tacgatggcc tttgcccgtt tttcagggtt ttttgatttg aagatacctg
     1981 atccaacgaa gacaccatcg cttccgagct gcatcatgat tgctgcgtct gcgggggtcg
     2041 caatcccacc tgcggcgaaa ttgacgacag ggagtcgttg cagttgcttg atttctttta
     2101 agatctcata gagttctttt ttaagggtgc tataggcgaa tgagccgaaa acaattgttt
     2161 tttcatcaac gttttgatcg gtcaatgttt tataggaaat gacataactt tgacaatacc
     2221 cctcagccac cttttgtatt tttgcgctgg gcattgtttt gagagtacta atcatgcttg
     2281 ttaccatgcg ctgatggcgc actgcctcga tgatattccc ggtgcctgct tctcctttgg
     2341 ttcgtatcat tgccgcacct tcccagatac gtcgtgtcgc ttcacctagg ttacgacaac
     2401 cgcatacaaa cgggatggta aaggctcgtt tgtcgatatg gtaaaaagga tctgc
//
LOCUS       DATMXJ010000134         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_60373, whole genome shotgun sequence.
ACCESSION   DATMXJ010000134 DATMXJ010000000
VERSION     DATMXJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60373"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VN377_03630"
     CDS             complement(<1..533)
                     /locus_tag="VN377_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799499.1"
                     /note="Converts oxaloacetate to phosphoenolpyruvate using
                     ATP as an energy source; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HWR27511.1"
                     /translation="MVQLHEMILRDGHQSLLATRMRTEDMLPIAEKLDEVGFFSLEVW
                     GGATYDVCIRYLNQDPWDRLNKLKKAMPNTPLQMLERAMNIVAYWNFPDDVVKKFIYL
                     AKKNGCDYFRIFDALNDLRNMKVPMQAAKENGGHVQACLSYTISPIHTVDYFVDKFIE
                     LKKMGADSVCIKDMAGM"
     gene            770..2821
                     /locus_tag="VN377_03635"
     CDS             770..2821
                     /locus_tag="VN377_03635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HWR27512.1"
                     /translation="MKHKIILILIGVFLLSTPVMPMSVGIPAGVISEGNISFDYRNVT
                     VYAPAVSQLDTGYVGVISTITVTVQGNGSGRVFVDTLPLTDVDMQGSARLAVKVATAL
                     VNMDTRPHLDPLTCDYFFVVRTASPMIGGPSAGAMMTVAVISLLENWTLDNTTVMTGM
                     INPDGSIGPIGGIPYKIDAAHSVGATRFLIPKGQTTYTEMITEYQNINGIIWQNTYPV
                     TRNVTEYAQTNYGMEVFEVEDINDALLYFTGWTFPVKESPDKITTEDYITAMKPLATS
                     LLDDARQSYQNASNLFNDTTIPNRYPNYYRNQVTDFLNTASDRLDESSHWYDEEVYYT
                     STSKSFQSLIDSHFVTHICRYFTSERQEELVQSLINQTVRNYQNNTELAKNASIHGMV
                     TLQCVGAAQQRISEAASYLSEANTSFLNADYFTALYNIAYAQERIESVQWWLNISTPF
                     HDSGEINETMITSLAEEYLDDAQQAIIYSEIILDEMGKTSDYLNDAKDLLETARSEQE
                     KGYSAAALFGSFETLVKANLALELVDGVTDDKVERARGQASVSITESRTIGIEPVLAV
                     SYYEYGQSLANESSLDTAIVYYKYADLIAGALRFTAPYGAESSRYVGVPDKNPVVTWN
                     FGLNRYIGLFLLFALIGGIAGLGFGILLAGLFGPKKQKEQLFPKDNVPRSIEDYYKKQ
                     K"
     gene            2845..3897
                     /gene="endA"
                     /locus_tag="VN377_03640"
     CDS             2845..3897
                     /gene="endA"
                     /locus_tag="VN377_03640"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HWR27513.1"
                     /translation="MDISGELINDHILVKKASDIGRLYNKSHIGTILPSNILRLSLLE
                     GVFLLDDGKMILHENKKQIQFSTLLKRAVQHIPEFETKYLVYKDLRNRGHAITQVDDI
                     SAISFRKLTTKNETTPSSVIAAYSERDVLDIEITKHLIQHYTKRNSALWFALVDEEGD
                     LTYYDIADVDLTGDIFEQKYTKGSAVLLKNRLIVFDKKLSSQLLQKEFFGKPFGETLQ
                     LSFVEALYLLEKKVLEIQTTDGTILSEEKCVQKMQKLQPDIVQRLIVFKDLKQRGLLV
                     KTGFKFGAHFRAYSKHPDKTHAEYLIHVIEKGFTSIWAEVSRAVRLAHSVNKEFIFAC
                     IDEGTIDYIRFGRLRP"
     gene            3965..>4256
                     /locus_tag="VN377_03645"
     CDS             3965..>4256
                     /locus_tag="VN377_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR27514.1"
                     /translation="MLQIRWHGHSCFEITNDVTIVTDPHDGRSIGIPAPNVTADIILV
                     SHNHYDHNSVKSVEKETSKVVTDERKRTIGNIQIMGIPAFHDAAHGERRGAMV"
BASE COUNT         1222 a          930 c          945 g         1159 t
ORIGIN      
        1 atcattccag ccatgtcctt gatacagact gagtcagcac ccatcttctt cagctcaatg
       61 aatttatcaa cgaaatagtc gacggtatga atcggtgaaa tcgtataact cagacatgct
      121 tgaacgtgac cgccgttttc cttagctgct tgcatcggga ctttcatgtt acgaagatca
      181 ttcagcgcgt caaagatacg gaaataatca catccgtttt ttttcgcaag gtaaatgaat
      241 ttctttacga catcatcagg gaagttccag tatgcgacaa tgttcattgc ccgctccagc
      301 atctgcagag gcgtgttcgg cattgctttt ttcagtttgt tcaatcgatc ccatggatcc
      361 tggttgaggt atctgatgca tacatcgtag gtggcaccac cccagacttc tagtgagaaa
      421 aacccgacct catcgagttt ttctgctatt ggaagcatat cttctgttcg cattcgtgtc
      481 gcgagtaacg attggtgtcc gtctcgcaga atcatttcat gtagttgtac catagtatgt
      541 tacctccagt tcccaacagg ttttttttca tctattgagt ggatggttta aacgagagat
      601 ggtatcctgt gaacctactt aagaatatcg gggagaaaac catggctttg aaacctatga
      661 tgtttataag ccatggaaaa aaattctcta aaaaatgatg caagaaccgc acagtaatct
      721 atttctataa gatgtactat gtaactcaaa ttcaattggg ggtgtgtcca tgaagcacaa
      781 gattatcctc atacttatag gcgtttttct cctcagtaca cctgttatgc cgatgtctgt
      841 cgggatacca gcaggggtga tttccgaagg aaatatctcg tttgactatc gtaatgtcac
      901 agtgtatgcg ccagcggttt ctcagttgga taccgggtac gtcggtgtga tctctacaat
      961 taccgtcacg gtccagggga atggaagcgg acgggtgttc gtcgatacac ttccgctcac
     1021 ggatgtggat atgcaagggt ctgctcgcct tgccgtaaaa gttgctaccg ccctggtaaa
     1081 catggatacg agaccgcacc ttgaccctct cacctgtgat tacttctttg tcgttcgaac
     1141 ggcatctccg atgattggtg gcccttctgc gggggcgatg atgaccgttg ccgtgatatc
     1201 cctcttagaa aattggaccc ttgacaacac cacggtcatg accggaatga tcaatccgga
     1261 tggaagcatc ggccctatcg gtggaatacc ctataaaatt gatgcagccc atagcgtggg
     1321 agcaacacgg tttttaatac caaagggaca aacgacgtac acagagatga tcaccgaata
     1381 tcaaaacatc aatggaatca tctggcagaa tacctacccc gtcactcgga atgtcaccga
     1441 gtacgcccag acgaattacg ggatggaagt ctttgaggtt gaagacataa acgacgcact
     1501 gctttatttc accggttgga cgttcccagt caaagaatcc cctgataaaa tcaccacaga
     1561 agattacata accgcgatga aacccttagc aacaagtctg ttggatgatg cacggcagtc
     1621 ttatcaaaac gcaagcaacc tgtttaacga cacaacgata ccaaacagat acccaaatta
     1681 ttacaggaat caggtgactg attttctgaa cactgcctct gatcgtcttg atgagtcatc
     1741 acactggtat gatgaagaag tgtattatac gagcacgagt aaatcattcc aatccctcat
     1801 tgactcccat tttgtcaccc atatctgtcg atattttacc tctgagcggc aggaagaatt
     1861 ggttcaatct cttattaatc aaacagttcg aaactatcaa aacaatactg agctggcgaa
     1921 gaatgcttcc atccatggga tggtgacgtt acaatgtgtt ggtgcagcac aacaacgcat
     1981 cagtgaggca gcatcgtacc tttctgaggc aaacacgagt tttctgaatg ctgattattt
     2041 cactgcattg tacaacatcg cctatgctca agagcggatc gaaagtgtcc agtggtggtt
     2101 gaacatctct accccgtttc atgattccgg ggagataaac gaaacaatga taaccagtct
     2161 tgcagaagaa tatctagatg atgcgcaaca agcgattatc tactcggaga ttatcctaga
     2221 cgaaatgggg aaaacatcag actaccttaa cgacgcaaaa gatcttcttg agaccgctcg
     2281 cagcgaacaa gaaaaagggt attctgctgc cgcattgttc ggctcgtttg aaacattggt
     2341 gaaagcaaat cttgccttag aactcgttga tggggtcacc gatgataagg tagagcgagc
     2401 tagaggacaa gccagtgtca gcatcacaga aagcagaacc attggaattg aacctgttct
     2461 tgcggtgagt tattatgaat acgggcagag cttagcaaac gaatcatccc ttgataccgc
     2521 gattgtgtat tataaatacg ctgatttaat cgcaggtgcg ttacgcttca ctgcccccta
     2581 tggtgctgaa tcttcccgct atgtgggtgt ccctgataaa aaccctgtgg taacctggaa
     2641 ttttggactg aaccggtaca tcgggttatt tctcctcttc gcattgattg gtgggatcgc
     2701 ggggctagga tttgggattt tactagctgg acttttcggt ccgaagaaac aaaaggagca
     2761 attgttccca aaagataatg tcccacgaag cattgaggat tattacaaaa aacaaaaata
     2821 agacatcgcc atatacctcg ttttatggat atctctggag agctcataaa cgaccatatc
     2881 cttgttaaaa aagcaagtga catcggaagg ctctataata aaagtcatat tgggacgata
     2941 ctcccatcga atatcttacg actgagtctg cttgagggtg tttttttact cgatgatgga
     3001 aaaatgattt tacatgaaaa taaaaaacag atacagtttt caacgctcct gaagcgtgcg
     3061 gtccagcata taccagagtt tgaaacgaag tacctggtgt ataaggattt acgaaatcga
     3121 ggccatgcga tcacccaggt cgatgatatt tcagctattt cctttcgtaa gctaacaacg
     3181 aaaaatgaga ctaccccctc cagtgtcatc gctgcgtatt ctgaacgaga cgtccttgac
     3241 attgagataa caaaacatct catccaacac tatacgaagc ggaatagtgc gttatggttt
     3301 gccttggttg acgaagaagg ggatcttacg tattatgata tagcagatgt tgatctcacc
     3361 ggggatattt ttgaacaaaa atatacaaag ggctctgctg tgttgttgaa aaatcgtctt
     3421 attgtctttg ataaaaaact ctcaagccaa ctgcttcaaa aagagttttt tggtaaaccc
     3481 ttcggagaaa cattgcagct ttcatttgtc gaggcgttgt atcttcttga aaaaaaagtc
     3541 ctggagattc aaaccaccga tgggactatt ctttctgagg agaaatgcgt ccaaaaaatg
     3601 cagaaacttc agccagatat cgttcagcga ctcatcgtgt tcaaagacct gaaacaacgg
     3661 gggctccttg tgaaaaccgg ttttaaattt ggcgctcatt tccgggcgta ttcaaaacat
     3721 cccgataaaa cacacgctga gtatctcatt catgtaatcg aaaaaggctt tacgagcatc
     3781 tgggctgagg tcagtcgtgc tgttcgtctt gcacattcag taaataaaga atttattttt
     3841 gcctgcatcg atgaggggac tattgattat attagatttg gacggctgcg cccataaatt
     3901 ttcataatgt atttatacct cgtcagccat tcggccaatt ccttatttga gggaggagat
     3961 tattatgctt cagatccgat ggcatggcca ttcctgcttc gagataacaa acgatgttac
     4021 gatcgtgacc gatcctcatg atggaagatc gatcgggatc cccgctccaa atgtcacggc
     4081 tgacattatt ttagtgtcgc ataaccatta tgatcataat agcgtcaaat ccgtggaaaa
     4141 agaaacgtcc aaggtagtca ccgatgagcg gaaacgaacc attggcaaca ttcagattat
     4201 gggtatccca gcgttccatg atgcagcgca tggagaacgt cggggggcca tggttc
//
LOCUS       DATMXJ010000135         6827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33953, whole genome shotgun sequence.
ACCESSION   DATMXJ010000135 DATMXJ010000000
VERSION     DATMXJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33953"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6827
                     /locus_tag="VN377_03650"
     CDS             <1..>6827
                     /locus_tag="VN377_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27515.1"
                     /translation="YYVRITNESLGLHKPEVLFQADIHGDEKVGAYGAYWFTDWLLRH
                     AYHPDYQDAQSEWLRWLLDNREIYIVPSMNPDGFDENHRGDNAGNDMNRQYDHSQSNP
                     WLSINTQTMRQFINNHVIRVAVDLHAGYRGIPYPWSNVNYRNDMSEQSAISGRTYIGQ
                     CPADFRFFDAAYLRLGTYIGNPSGAGAFTSSNIGPWTHTLGYDADGTSCDWWYGGNVL
                     ATPSEDPYVNDETFGNYPGCGIMATLVEYGPGNPTESQLGNDTNNKFGAEVRRTLLHQ
                     IDLAQPYLRWQGGTVENDAVVDVGDTVSLTWQVNGSLVVDNTLVQWGLNPDPVDYPQN
                     YLSNHQDHEGDYYGGTGWDNAINGNTSGARYTETITLNAPGDYYFVAKAKVDQRYSSV
                     IYPDEYGNTSYLRIIKERTDDSFYEQINGTDGIEIIDGQVWWYSPVIHVRAGPPVHQK
                     IITVNHSKVLASLQNYPLFINLDSDTDLAFYAQDDGDDITFYDAAQNKLNHEIELFNG
                     TTGQMIAWVNVPYLSSIEDTVLYMQYGNETSGNQQNPEGVWDSVYKGVWHLGEDFLDS
                     TSNNNDATNYGSTDAAGAINDGQSFDGNDYITAPSHCLGADHNWTASCWFRSLNSTML
                     FHYFFSTGGYNAADAVNIWQNINQALDIGEVRSQIVDSTGDRIDQFNYGGTSYKDQSW
                     HLASVTWDAPLHTLALYVDGSLENISTNSIVDTGAGTSTPLYIGARTDLEAQRFHIGG
                     LDEVRIMNTRKNASWITTEYNNQHDPSQFYTIGIAAFEAPALFDENPSDESIDIGLNP
                     MLSISVMDYQEDLMDIFFKSNSSGSWSTLGSIVDVGDGTYYQTTNDMSAFSTTYYWSV
                     NCTDGTHWTNLTYRFTTEAAPPPWWNSSWEYRREITIHHTLVKGDLVNFPVLISIPTD
                     SDLAEKAQNDADDLVITDYYGNRLNHEIELFNENTGQLVVWVNVTSLSKISDTNLFLY
                     YGNLLCGNQQYPTAVWDSNYVAVWHLSEDPSVTGTGGIKDSTSYVNHGTDQGGMDSSD
                     HISGMIGYGVDFDGTNDVIKIENSWTTGHSLDLTTGPMTLETWFKCHVESNQGTLISK
                     RDGTTDDQYQFFVQPQLQFRANQEYGSGTDSLTTEIWYYGAVVVNSTNWPAIYRDGVM
                     KTWADIAGSRPYDFVHRNVNVSIGARWQVYPTPGYPFNGVIDEVRISKIDRSSEWLNT
                     CYNNQYNPFGFSALGTEESKSGVPSLSSETPLNNAVGQPLNPVLAVTVTDLQGDLMDI
                     LFRTNASGSWQTIGEYTSVGSGRYSQGSTNMNQYLTRYWWSLNVTDQGSHTWKNKTYV
                     FSTKTNAPNIATPSPANGSIDVGGNPLLSISLVDYEGDLMTVIFRSNQSGVWAVIGVY
                     NNVTNGTTKHQTVDMINPSTTYWWSVSVTDSGSGNWSNATYRFTTTDKMMKLKWMKTN
                     LPQPNGGGDLLADISGDGIDDVIHTGTGGVVVLDGIDGHTIWSRSDGSITPDVRPEMA
                     DLNNDGVFDVIVPLSNGAHVFYGNNGTTYWRINNLRGSAVFSSLIAYDIDGSGYPYVF
                     IPVQDIDEPLDGTLNMISHDGKLLRSTWVYRPCWGGLSLADADFDGRFELYICDRNSD
                     AGKGLRSFWASNLTCRWNRTDIFDSSSIPMIADVNNDGRLDIITPHQVSPYGLLVANF
                     DGTTIKKETGITIPGHYPPSVCDIDRDGHFEILMANPYGEATYDVVVWDLDPVAWGMD
                     ARFNVGVCEFGPKCADVDGDGYPEIIAVSSNGIYIYNSTFNRTYQITGLTGRLQYATA
                     NDIDGDGLNEVVVPSTAGIVYAFDTFAPTPNPRPRSEVQFYSEYRRGVAEYLDRPGVK
                     YPMQRDEYPANGSSFISPNPTLSAWVWDYQWDEFNITVSTNASGSWQTLASFTHIGRY
                     PGKTLTGNAWGRYSVPTTTMTQPYATYYWRIFAVDVKGNSFQKTYRFTTADVAPTVSN
                     PNPAEGKTGLPLSLSTLRFTLMDFQGDKMNYTVQTRPNIGSGQGTNVGNGNYSVSVGG
                     LSYNTTYTWFVNVTDGNGHWTNKTYPFTSRPSPGVWWNPGWMYRKELILNNTKIAGLL
                     SNFPILISLGADANLIAHAQPDGDDLVVTDYNRHPLNHEIELYNATTGKLVIWVNVTS
                     LSPTSTIILYMYYGNPSCASQQHPTGVWHSSYLAVHHLEETNGTVFDSTIRHRDGTPL
                     GGLQQIAIGIIDGADTFDGTNDYIDLGTTTDFNKQDLTVSAWVKSSVPTTDMRIVVGG
                     ASYTYKWHLIMDDGYFMMTNNTLATTN"
BASE COUNT         1865 a         1426 c         1664 g         1872 t
ORIGIN      
        1 gtattatgtc cgtatcacca atgagtccct tgggctccac aaacctgaag ttttgttcca
       61 agctgatatt catggagatg aaaaggtcgg ggcatacggt gcgtattggt tcacggattg
      121 gttgctaagg catgcatatc atccagacta tcaggatgcg cagagtgagt ggctccgttg
      181 gttacttgac aatcgtgaga tctacattgt ccctagcatg aatcctgatg gttttgatga
      241 gaaccatcga ggggataatg ctgggaatga tatgaacaga caatatgatc atagtcaatc
      301 aaatccttgg ctttcgatta atactcaaac catgcggcaa ttcatcaata accatgtgat
      361 tcgggtcgcg gtggacttac atgctgggta tcgggggatt ccctatcctt ggtcaaatgt
      421 aaactatcgg aatgatatgt cggagcagag tgcgatctct gggaggacat atatcggaca
      481 atgtcctgct gacttccgat tttttgatgc agcatatctt cgattaggga cttacatcgg
      541 caatccatcg ggtgcagggg catttacaag cagcaatatt ggcccatgga cccatacgct
      601 tgggtatgat gctgatggca ccagctgtga ctggtggtat ggcggtaatg tgctcgcgac
      661 accttcggaa gacccgtatg tgaatgatga aacctttggg aactatcccg ggtgcggcat
      721 catggctacc cttgtggagt acggtcctgg taatcccaca gaaagtcaat taggtaatga
      781 tacaaataat aaatttggtg cagaggtgcg tcgtacttta cttcaccaaa tcgatctcgc
      841 acaaccgtac ctccgctggc aaggagggac agtggagaat gatgcggtgg ttgatgttgg
      901 agatacagta tccttgactt ggcaggtgaa tggtagttta gtcgtagata atacacttgt
      961 acagtgggga ttgaatccag atcccgtgga ttatccgcag aattacctgt cgaatcatca
     1021 ggatcatgag ggagattatt atggagggac tgggtgggat aatgcaataa atggaaatac
     1081 atctggggct cgatataccg aaacaattac gttgaatgct cctggtgatt actattttgt
     1141 tgctaaagcg aaagtagatc aacgatactc gtctgtgatt tatcctgacg agtacggaaa
     1201 tacctcatat cttcgaatta taaaagaacg gaccgatgat tccttttatg aacagattaa
     1261 tgggactgat ggaattgaaa ttattgatgg gcaagtatgg tggtatagtc cagttattca
     1321 tgttcgtgct ggacctccag tgcatcagaa aatcatcaca gtaaatcata gtaaggttct
     1381 cgcatcctta caaaattatc ctttattcat caatttagat tcagacaccg atcttgcgtt
     1441 ctatgcacaa gatgatggag atgatatcac gttttatgat gcagcgcaaa ataagttgaa
     1501 tcatgaaatc gaattgttta atgggacgac agggcaaatg atagcctggg tgaatgtgcc
     1561 ttatctctcc tcaattgagg ataccgttct ctatatgcaa tatgggaatg agaccagtgg
     1621 gaatcagcag aacccagagg gagtgtggga ttctgtgtat aaaggggtct ggcatctcgg
     1681 ggaagatttt cttgatagta caagtaataa taatgatgca acaaactatg ggtcgactga
     1741 tgctgctggg gcgattaatg atggtcaatc gtttgatggg aatgattata ttactgcacc
     1801 atcgcattgt cttggtgcag atcataactg gaccgcgagc tgctggttcc gatcattgaa
     1861 ttccacaatg ttgttccatt atttcttctc taccggtgga tataacgcag ccgatgcggt
     1921 gaacatctgg cagaatatca atcaggctct tgacattggg gaagtacgtt ctcagattgt
     1981 tgattccaca ggggatcgta ttgaccagtt taattatggg gggacaagtt acaaggatca
     2041 atcctggcat ttagcctcgg ttacctggga tgcaccgtta cataccctgg ctctctacgt
     2101 cgatgggtcc ttagaaaata tctctacaaa tagcattgtg gatactggag caggaactag
     2161 tactccgctc tatatcgggg ctcgtactga tcttgaagct caacgtttcc atatcggagg
     2221 tctcgatgaa gtacgcatca tgaacacaag aaaaaatgca agttggatta ccaccgaata
     2281 caacaaccag catgatcctt cacagtttta tacaattgga attgcggcat ttgaagcgcc
     2341 tgctcttttt gatgaaaatc catccgatga gtctatcgat atcgggttaa accccatgtt
     2401 atctattagc gtgatggatt atcaagagga tttaatggat attttcttta agtcaaactc
     2461 ttcaggtagt tggtcgacac tcggcagtat cgtcgatgtg ggagatggaa cctattatca
     2521 gacaacgaat gatatgagtg cttttagtac cacctactac tggagtgtga attgtaccga
     2581 tgggactcat tggacaaacc tgacataccg gttcaccact gaagcagccc caccaccctg
     2641 gtggaactcc agctgggaat atcgacggga aatcacgatt catcatacct tagtgaaggg
     2701 tgatttagtg aatttcccag ttcttatcag tatcccaact gattctgatc ttgcagaaaa
     2761 ggctcaaaac gatgccgatg atctggtaat tactgattat tatggaaata ggttaaatca
     2821 tgagatagaa ttatttaatg aaaacacggg gcaacttgtc gtatgggtga atgtcacgag
     2881 tctctcgaag atatcggata ccaacttgtt cctttattat ggaaatttat tatgcgggaa
     2941 tcagcagtat cctactgccg tctgggattc taattatgta gcggtttggc atctcagtga
     3001 agatccttct gtcaccggga ctggaggaat caaagattct actagttatg taaatcatgg
     3061 gactgatcaa ggtggcatgg attcttctga tcacatcagc gggatgattg gatatggtgt
     3121 cgatttcgac ggaaccaatg atgttattaa gattgagaac tcctggacca ccggtcatag
     3181 ccttgatctt acgactggcc cgatgacact tgaaacatgg tttaaatgcc atgtggaatc
     3241 aaaccaaggg accttaataa gtaaacggga tggtactaca gatgatcagt atcaattttt
     3301 tgtccagcct caactgcaat ttcgagcgaa tcaggaatat ggatccggaa cagactctct
     3361 cactacagaa atctggtatt acggcgctgt cgttgtaaat tcgacaaatt ggccagcaat
     3421 ctaccgtgac ggagtgatga aaacatgggc tgatatcgcc ggatcccgac cctatgattt
     3481 tgttcataga aatgtcaacg tttcaattgg tgcccgatgg caagtgtacc cgacgcccgg
     3541 atatcccttt aacggagtta ttgacgaagt tcgaatttca aagatagacc ggagcagtga
     3601 atggctcaat acatgttata ataatcaata taatcctttt gggttctctg cacttggaac
     3661 ggaagaatca aaaagtggcg ttccctcgct tagcagcgaa actcctttga acaatgcggt
     3721 tggtcagccg ctaaatcctg ttctcgctgt gactgtgact gatttacaag gggatctcat
     3781 ggacatccta tttaggacca acgcaagtgg cagctggcaa acaatcggtg aatacacctc
     3841 tgttgggagc ggaagataca gtcaaggatc aaccaatatg aaccagtatc tgacacggta
     3901 ttggtggagc ttgaacgtca ctgatcaagg ttctcatacc tggaaaaata aaacctatgt
     3961 cttttcaaca aaaacaaatg ctccaaatat cgcaactcct tcgccagcga atggatccat
     4021 cgatgtaggt ggaaacccac ttctttctat ttctttagtt gattacgaag gggatctgat
     4081 gacggtgatc ttccgatcaa accagagtgg cgtctgggca gtcattggag tgtacaacaa
     4141 tgtcacgaat ggaacgacaa aacaccagac agtggacatg attaacccca gtaccaccta
     4201 ctggtggagc gtcagtgtca ctgatagtgg gagtgggaac tggtccaatg cgacataccg
     4261 gttcacaacc acagataaaa tgatgaaact caaatggatg aaaaccaatt taccccaacc
     4321 aaacggtgga ggtgatctcc ttgcagatat atctggtgat gggatcgatg atgtgatcca
     4381 tacagggacc ggtggagtag tcgtattgga tggtattgat ggtcatacga tttggtctcg
     4441 ttctgatgga agtatcacac cggatgttcg acccgagatg gcggatctta ataacgatgg
     4501 ggtgtttgat gttatagtac ccctttcaaa tggtgcccat gtcttttacg gaaataatgg
     4561 aacaacgtac tggcgtatca ataatttacg gggttctgca gtgttcagca gtttgattgc
     4621 ctatgatatc gatggttcag gttatcccta cgtgttcatt cccgtgcaag atatcgatga
     4681 acctctggat ggaaccttaa acatgattag tcatgatggt aaattattac gatcaacttg
     4741 ggtatatcgt ccttgctggg gaggattatc ccttgctgac gcggactttg atggacgatt
     4801 tgagctgtac atctgtgatc ggaatagtga tgctgggaaa ggacttcgct ccttttgggc
     4861 gagtaatctg acttgccgtt ggaaccgcac agatatcttc gacagtagtt ctattccgat
     4921 gatagcggat gtgaataacg atgggagact cgatatcatt acacctcatc aggtgagtcc
     4981 gtacgggttg cttgtcgcca acttcgatgg aacaaccata aaaaaagaaa ctgggattac
     5041 tattcctggg cattatccgc cttcggtctg cgatatcgac cgtgatgggc attttgagat
     5101 tctcatggcg aacccgtatg gagaagccac ctatgatgtc gttgtttggg atcttgaccc
     5161 tgttgcttgg gggatggacg ctcggtttaa tgttggggtg tgtgagttcg gtccgaaatg
     5221 cgctgatgtt gatggcgacg gatatccgga aataattgcg gtgagttcca atggtattta
     5281 tatctataac agcacgttta accggacata ccaaatcact ggtctgaccg ggcgcctgca
     5341 gtatgcaacc gctaatgata ttgatggtga tgggttaaat gaggttgtgg ttccgagcac
     5401 cgcagggatt gtctatgcgt ttgatacgtt tgctccgaca ccaaatcctc gaccccgctc
     5461 tgaagttcag ttttatagcg agtaccgacg aggagtagcg gaatatctag atcgacctgg
     5521 agtaaaatat ccgatgcagc gcgatgaata tcctgcgaat ggatcttcgt ttatttctcc
     5581 gaatccaacg ctttctgctt gggtctggga ttaccaatgg gatgaattca acatcactgt
     5641 gagcaccaat gcgagcggca gctggcaaac cctcgcatcc tttacccata tcgggcggta
     5701 ccctgggaaa accttgacag ggaacgcatg gggacgatac tcggttccaa cgacaactat
     5761 gacgcagccc tacgctactt attactggcg aatctttgca gtggatgtaa agggaaacag
     5821 cttccagaaa acgtatcgtt tcacaaccgc agatgttgcc cccacggtat caaatccaaa
     5881 tcctgcagaa ggaaagactg ggcttccatt atcattatcg acccttcgat ttacccttat
     5941 ggatttccag ggtgacaaaa tgaattacac ggtgcagact cgacccaata tcggatctgg
     6001 tcagggaacc aatgtcggga atgggaatta tagcgtctca gttggtggtt tgagctacaa
     6061 caccacctac acctggtttg tgaatgtcac cgatggcaac gggcattgga cgaacaaaac
     6121 gtatcctttc acctcacgtc cctcaccagg ggtctggtgg aatcctgggt ggatgtaccg
     6181 gaaggagctg attcttaata acaccaaaat cgctggttta ctctcaaact tcccgattct
     6241 tatctctctt ggtgctgatg ctaatctaat cgctcatgcg caacctgatg gtgatgatct
     6301 tgttgtcaca gattataaca gacatccgtt gaaccatgaa attgagttat acaatgcaac
     6361 cacagggaaa ctagtgatct gggtgaatgt caccagtctt tctccgacat ccactatcat
     6421 cctctacatg tattatggga acccttcatg cgcttcacaa caacatccga ctggtgtttg
     6481 gcattcatca taccttgcgg tacatcatct tgaggaaacc aatggcacgg tctttgacag
     6541 tacgattcgt catcgtgatg gaactcctct gggtgggcta cagcaaattg cgatagggat
     6601 tatcgacggg gcggacacgt ttgatggaac aaacgattac attgatcttg gtacgacgac
     6661 agatttcaac aaacaggatc tgaccgtctc cgcctgggtg aaatcctccg tacccaccac
     6721 ggatatgagg atcgtcgtcg gtggagcttc ctacacctac aaatggcatt tgatcatgga
     6781 tgatggctat ttcatgatga cgaataacac gcttgcgacc acgaacc
//
LOCUS       DATMXJ010000136         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_33963, whole genome shotgun sequence.
ACCESSION   DATMXJ010000136 DATMXJ010000000
VERSION     DATMXJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33963"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VN377_03655"
     CDS             <1..626
                     /locus_tag="VN377_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWR27516.1"
                     /translation="LFQTVKTFTSTVLGKQKIPIALGGDHSITPGIVSAFPKDIAVLS
                     LDAHMDYRQEYKNDIYNHACVIRRITDHLPLQNIAVLGLRSAEKEEYETALKQGLFFR
                     DAYTINTIGLKKTIQQTKTLLKGKKIYLTLDIDVIDPAYAPGTSTPEPFGLTPIQVME
                     IIEAFSPQLIGCDLVEVCPPYDQGQTSVLAAKLINTCLSLIWKEHQD"
     gene            659..1789
                     /locus_tag="VN377_03660"
     CDS             659..1789
                     /locus_tag="VN377_03660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWR27517.1"
                     /translation="MQKEKIALIALVIIVVAALSVFVAAVNTDIFENLFKEKLTIAEG
                     DCADVNYIGRYASNNTVFDSSYNNTANKTGGTPLKVFVSYNTTATSPKTGYTAGMIKG
                     FMQGIIGMKEGQTKIIGPIPPEDAYGTNKFGVGAIFTSQYFAFGMNQTVEVTNYTNEN
                     LTVRWIQMENLRNFTMPQLVIDNLQSTNETEMVIYPPPYYIWENATVITNITDTTVTV
                     RTTPTKSTNLSDIVKDVRYGEKQMLIFPNATTASWDNTTITIVCSPIVGRNYTFQTAG
                     YTGMVNITIHVNTIIGDQINVTITNDQSAEPSYLDVYKVLTFNRTFTLPRIYKDIPAM
                     YISYFYGEDIKKAGYSIEPLAGETLLFEVTVEKVYKTSQETS"
     gene            complement(1774..>2582)
                     /locus_tag="VN377_03665"
     CDS             complement(1774..>2582)
                     /locus_tag="VN377_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWR27518.1"
                     /translation="RPLAKLLQYATDPLIPGVSGREDSSVGFLQELQIELKERDTWRT
                     WVDLRKEEKRKIISSIVQMLLSKGFGYQVASRVLGVSYLLCKEEEGTELHDAKEYATL
                     LNSTARYGQYEVGLQVCLGDRGKWLKEALNLLSGHRHNLVEGLQFAREEKIQKRSYVQ
                     YFHAGDGIRDTIIGIVTNMLLNTEDVDKELPLIGFAHTETGEVKVSARATQALVDRGL
                     DLAVALTRAAKELNGVGGGHNIAAGATIPKGKEEEFLRVVEREIKLQLVS"
BASE COUNT          805 a          678 c          523 g          576 t
ORIGIN      
        1 agctatttca gacagtaaaa acattcacct cgacagtact aggaaaacag aaaataccga
       61 tcgctctcgg aggggatcat tccatcaccc cagggattgt ctcagcgttc ccaaaagaca
      121 tcgcagtcct ctcactcgat gctcacatgg attaccggca ggaatacaaa aacgatatct
      181 ataaccatgc gtgcgtgatc cgtcggatca ctgatcatct tcctctgcag aacatcgcag
      241 tgttgggcct acgatccgca gaaaaagaag aatatgagac cgccctaaaa caagggcttt
      301 ttttccgtga cgcatatacc atcaacacca ttggtcttaa gaaaaccatc caacaaacaa
      361 aaaccctact gaaagggaaa aaaatctatc tgacattgga catcgatgtc atcgacccag
      421 catacgcacc cgggacaagc acaccagaac cctttggact gaccccgata caggtgatgg
      481 agatcatcga ggcgttctct ccacagctga tcggatgcga tctagtagaa gtctgcccgc
      541 catacgacca gggacaaacc tctgttcttg cagcaaaatt gatcaacacc tgtctgagtt
      601 taatatggaa agaacatcag gattagatta atatatatca atttcattca gcacgctcat
      661 gcagaaggaa aaaatcgcac taatcgcgct agtaatcatc gtcgtcgcag cactatcagt
      721 ctttgtagca gcggtcaaca cagacatttt tgaaaatctc ttcaaggaga aactgacgat
      781 agcagagggc gattgtgcag atgtgaacta cataggacga tacgcatcaa ataacacggt
      841 ttttgattca tcatataaca acactgcgaa taaaacaggc gggacaccac taaaggtgtt
      901 tgtcagttac aatacaacag ccacatcccc gaaaacaggg tataccgcag gaatgatcaa
      961 aggattcatg caaggaatta tagggatgaa ggaaggtcag acaaaaataa ttggtccgat
     1021 ccctcctgaa gacgcatacg gaacgaacaa atttggtgtt ggggcgattt tcacaagcca
     1081 gtacttcgcg tttgggatga accagaccgt ggaagtgacg aactacacca acgagaacct
     1141 gacagtcaga tggatccaga tggaaaacct gaggaatttt acgatgcctc aactcgtcat
     1201 cgacaatctt cagagcacca atgaaacaga gatggtcatc tatccgcctc cctactacat
     1261 ctgggaaaat gccaccgtca taacgaacat cactgacaca actgttacgg tgaggaccac
     1321 accaacaaaa agcaccaatc tatcagatat cgtaaaagat gtgcgatacg gagaaaaaca
     1381 aatgctcatc ttcccgaacg caaccaccgc atcatgggat aatacaacaa taaccatcgt
     1441 ctgttcaccc atagttggta ggaattacac ctttcaaacc gcaggataca ccgggatggt
     1501 caatatcacc atccatgtca ataccatcat cggagaccag atcaatgtca caatcaccaa
     1561 tgaccagagc gcagaaccca gctatcttga cgtctataaa gtgttaacgt ttaataggac
     1621 gtttaccctg ccacgcatct acaaagatat cccggccatg tacatctcct atttctatgg
     1681 agaggatatc aaaaaagcag gatacagcat agaaccgctc gcaggggaaa cattactctt
     1741 cgaagtcacc gtcgaaaaag tgtacaagac atctcaagag acaagctgaa gttttatttc
     1801 acgttccact acacggagga actcttcttc ttttcctttc ggaatcgttg ctccagcagc
     1861 aatattatga ccgcccccga caccgttcaa ctccttagct gcacgggtaa gagcgacggc
     1921 aaggtccaat ccacggtcaa ccaaggcttg tgtcgcccgg gcagaaacct tcacctcacc
     1981 agtctcagta tgcgcaaatc cgataagcgg gagttcttta tccacatcct cggtattcaa
     2041 cagcatattt gtgacaatcc caatgatggt atcacggatg ccatcaccag catggaaata
     2101 ctgaacatag gagcgtttct ggattttttc ttcccgagca aactgaagac cttcgaccaa
     2161 gttatgccga tgaccactga ggaggttcaa cgcctctttt aaccatttcc cacgatcccc
     2221 cagacagacc tgaagaccga cctcatactg gccatagcgt gccgtagagt tcaaaagcgt
     2281 agcgtactcc tttgcatcat gaagctccgt gccttcttcc tctttacaga ggaggtatga
     2341 tacccctaac acccgcgagg cgacctgata accaaaacct ttcgaaagca gcatctgaac
     2401 aatcgaagag ataatcttcc gtttctcctc tttccgaaga tcaacccatg tcctccaggt
     2461 atctctctcc tttaattcta tctgaagctc ctgtaaaaaa ccgacagagg aatcctcccg
     2521 accgctcaca ccagggatca acggatcggt cgcgtactga aggagtttcg caagcggccg
     2581 tg
//
LOCUS       DATMXJ010000137         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_18517, whole genome shotgun sequence.
ACCESSION   DATMXJ010000137 DATMXJ010000000
VERSION     DATMXJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18517"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..632)
                     /locus_tag="VN377_03670"
     CDS             complement(252..632)
                     /locus_tag="VN377_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27519.1"
                     /translation="MKETTIFREALGDSPMIRVLDFLIEGRGLDYSLTDIAENANIGW
                     TTLHRMWDTLLRLKMVVPTREIGRAKLFKLNQENLSVERLIKYYDTLLYLETEKYFAK
                     EVVAIIPKIATKRNGKTESIVAHS"
     gene            744..1007
                     /locus_tag="VN377_03675"
     CDS             744..1007
                     /locus_tag="VN377_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27520.1"
                     /translation="MQKRDIQEHILRKLMARRKWGGSHTEETNLTKGLPSNLIGQKIV
                     EEAIDELYQKEFLHKSKKTKEWHVSLNPQKKKEIYRFLGIPPK"
     gene            complement(1046..1729)
                     /locus_tag="VN377_03680"
     CDS             complement(1046..1729)
                     /locus_tag="VN377_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWR27521.1"
                     /translation="MTKTIVVNGCLRMEKSDTAKILTPFLEGMKQAGADVEMFYLKRM
                     NIQPCEGDFACWSKNPGICSIRDDMQLLYHKMREAEILVVATPVYIPLPGQMQNVLNR
                     MVALLDPVVKTRKGRTRAKFREEVKIKKMVLVSSSAWWEKANFETVERIVQELAEDAS
                     VKYVGAILRPHVQWLVEKSEKAEEISSASRHAGFQLIKKGTMSKNDLNIISQPLVSNR
                     RFLEAERNA"
     gene            complement(1827..2282)
                     /locus_tag="VN377_03685"
     CDS             complement(1827..2282)
                     /locus_tag="VN377_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27522.1"
                     /translation="MKKQFVIIGVIAILVCVGLSGCNSETEKDLENNRTGMPFLPPDF
                     RVSGLTVMDDCERVYPDLGTAYVNFTVTNHGSPGARYAFCRVYQGIENFQLCGNGIIY
                     NNTRSSYLQLENEESKQVSFVFPGIDCTNGTGCWGYEYWISESSLSTGP"
     gene            complement(2640..3089)
                     /locus_tag="VN377_03690"
     CDS             complement(2640..3089)
                     /locus_tag="VN377_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27523.1"
                     /translation="MKKTWFVLPGYCVGLAGLLLITYRTLLAVSSPEKSILVFVNRFG
                     EQYLDLVCLVFLWVVCLVGLLCLSSWVSEMGRRERSQAEQTRKGVLRSPVLSFDVLDS
                     FHQRSALPMMGDVGEGFYTAEETSLSVDETAGSVSVSIVIQQEDSQG"
     gene            3298..>4389
                     /locus_tag="VN377_03695"
     CDS             3298..>4389
                     /locus_tag="VN377_03695"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27524.1"
                     /translation="MKKNPLLRTKSKHPITKPVLYTTTFLLLAVCLLPLAISTTPAPS
                     IRLPDRPVTMIVTDGQHSYFDIDLSDVPPNLDVANQEYKGWCADRFVTMPRNQELTVR
                     LYNNYDLSLPVPLRENWSKVNYILNHHDGATKIDVQGAFWYLLCDYPYSSLTSTAKIL
                     VDTAQNEFIPQSGEWIAILAEPIQNDSHPWPFQFSFLQVRLPSQGPEPVEPTQTTTTI
                     SHGYRYNDIAPTADTNGPYSGYAKETVEFSAAASVDPDGIIILYKWSFGDGTTAEGTT
                     ATHTYSHAGTYQVRLKVTDNFGLSDTDVTNATITVRNNPPTPFINGPVNGTTNTIYAY
                     AFSATDQEPLITYQINWGDGTPTQTATHPS"
BASE COUNT         1323 a         1113 c          754 g         1199 t
ORIGIN      
        1 gttttacgca atggcttttg tgcgaaccat agataaaaat gagaaaatat aattatggat
       61 tagttccctg gcagggattt taatttcatt atatcctaaa atctctgtca attattacca
      121 tgataaagaa tggtaggcca gaaccgggct tatgctctaa aggttcatta ttttgtatta
      181 taatctgtta aatcccttca ttaagacctt tgattttctg ttattttctt taaaaatgat
      241 agttttagat actatgaatg ggcaacgatt gattcggttt ttccgtttct ttttgttgct
      301 atctttggga taatagctac tacttcctta gcaaagtatt tttcagtctc aagatagagt
      361 aatgtatcat aatattttat taatctttcc actgacagat tttcttgatt taatttaaac
      421 aacttagccc ttccaatttc tctcgttggc acaaccattt tcaatcgtaa tagagtatcc
      481 cacattctat gtaaggtcgt ccatccaata ttcgcatttt cagcgatatc agttaatgaa
      541 taatccaatc ctcgcccctc aattaaaaag tcaagaactc taatcatagg cgagtctccc
      601 aatgcctctc taaatatagt tgtctctttc atcaaataat aatgtacttt gcatcactat
      661 ataaaacttt ctattttggt atattaatat accaaaaaat tagataaact ttataagttt
      721 ttatgattta actttaatag aaaatgcaaa aaagagatat tcaagaacat atcttaagaa
      781 aattaatggc aaggcgtaaa tggggaggtt ctcacactga agaaacaaat ttgacaaagg
      841 gtttaccatc gaatttaata ggacaaaaga ttgtagaaga ggctattgat gaattatatc
      901 aaaaagagtt tcttcacaaa tcaaaaaaaa caaaagaatg gcatgtttct ttaaatcctc
      961 aaaagaaaaa ggagatatac agattcttag gcatcccccc taaatgattt ttttcagcat
     1021 ttcttcattt tgttagaaag attggttagg cattccgttc agcttccaag aaacgcctgt
     1081 tcgaaacaag cggctgactg atgatgttca gatcgttctt tgacatcgtt ccttttttga
     1141 tgagctggaa accagcatgt cgagatgcgc tagagatttc ttctgctttt tctgatttct
     1201 caaccagcca ttgcacgtgt ggtcgcagta tcgctcctac gtacttgacg cttgcatcct
     1261 ctgcaagttc ctgaacgatt ctttctacgg tttcaaagtt tgctttttcc caccatgccg
     1321 aagaggatac gagaaccatt tttttgattt tgacttcttc tctgaatttg gctcgcgtgc
     1381 gacccttgcg ggtttttacc acagggtcta acaaggcgac catcctgttt agcacattct
     1441 gcatctgtcc aggcaatggg atgtacaccg gggtggcaac gacaagtatc tcggcttcac
     1501 gcattttatg atacaggagc tgcatatcat cacgtataga acagatccct gggtttttac
     1561 tccagcaagc gaagtcccct tcacagggct gtatattcat acgtttgaga tagaacatct
     1621 cgacgtcagc ccccgcctgt ttcatgccct caagaaaagg agtcaggatt ttggctgtgt
     1681 cgcttttttc catcctcaga cagccattca ctacaatagt ttttgtcaaa acctttgcct
     1741 ccaatctgat attttgtaat tattccttct ttaaaaacat atctttagaa acagaagcat
     1801 tgacactgtt ttaacgaatg tttctactat ggtcccgtgg ataatgaaga ttcggaaatc
     1861 caatactcat aaccccaaca ccctgtacca ttcgtgcagt cgatacctgg aaaaacaaat
     1921 gagacctgct ttgattcttc attctctaac tgtagatatg acgagcgtgt attattataa
     1981 ataattccat ttccacaaag ctgaaaattt tctatccctt gatacactct acaaaatgca
     2041 tagcgcgcac ccgggctgcc atgattggtg accgtaaaat tcacataggc tgtacctaaa
     2101 tctggataca cccgctcaca atcatccatt actgttagcc cacttactct aaaatcaggg
     2161 ggaagaaaag gcatcccggt tctgttgttt tctaaatcct tttctgtttc cgaattacac
     2221 ccactcaacc caacacaaac gagaatggca atgaccccga tgattacgaa ttgttttttc
     2281 attaagcgtt tgcctccact ctgttatttt gtaacaattc cttctttaaa aacatatctt
     2341 aagaaccaaa cggacatcga cagggctcat ccaaaataaa gtgaaaaaaa aaaattacta
     2401 atctcagagt gtgatctgat gatacgtgtt ttctttttat tgtatcattc agcacttttc
     2461 gtcttcatcc cagacatacg cttcatcccg gaacttgtca gggacttcct tgattcgttt
     2521 gagtttgcct tgttttgata tcttcacgaa cgcaaccatt tgtctcacct gttgtcatac
     2581 attctgacga tagtgctgtt gatttgtggg tatttatatt tttcccaaaa atattttttt
     2641 tatccctggg aatcctcctg ttggatgacg atcgacaccg agacactccc cgccgtctca
     2701 tccacagaaa gagacgtttc ctccgcagtg taaaaccctt cgcccacatc tcccatcatt
     2761 ggcaacgcag accgctgatg aaacgaatca agcacatcaa aagacaacac cgggctcctc
     2821 aacacaccct tccgcgtctg ctcagcctga gacctctccc gccgtcccat ttcacttacc
     2881 caggacgaca ggcacaaaag accaacaaga cagaccaccc acagaaacac cagacacacc
     2941 agatccaggt actgctcccc gaaccggttc acaaaaacca gaatgctctt ctccggagaa
     3001 ctcaccgcaa gcaacgtccg atacgtaatc aacaacagac cagcaagacc aacgcaatac
     3061 cctgggagga caaaccatgt tttcttcatc aaaccaattc ccagaatacg aaaattaata
     3121 tataaaaccc actgataaat acctgctttt ttcctccaga acgaccaccc ggcattcact
     3181 ccaggggacg gaaccgatac cgattgtaca gacactggat aacaggtgta caacagatgc
     3241 ttaataacag aaggaacaac acaacaccat agaatgaaaa aaaggatgga gaaaagaatg
     3301 aaaaaaaacc ctctgctcag aaccaagagc aaacacccaa ttacaaagcc ggtgctctac
     3361 accaccacgt tcctgctgct cgccgtctgc ctcctgccac tcgcaatctc aacaacacca
     3421 gccccgagca tccgcctgcc tgacagacca gtgaccatga tcgtcaccga tggacagcac
     3481 tcgtatttcg acatcgatct ctctgatgta ccaccaaatc ttgacgtggc aaatcaagag
     3541 tataaaggat ggtgtgctga tcgattcgtc acgatgcctc gaaaccaaga actcacggtg
     3601 agattataca acaactatga tctttcatta ccagttccat tacgtgaaaa ctggagtaaa
     3661 gtaaactata ttctgaatca tcacgatggt gcgacaaaga tagacgtcca aggtgcattt
     3721 tggtatttac tgtgtgatta tccgtattct tcactgacaa gtactgctaa aatattggtt
     3781 gataccgctc aaaatgagtt tatccctcaa tctggtgaat ggatcgccat acttgcagaa
     3841 cctatacaaa atgactcaca tccatggccc ttccaattct cgtttttaca agtcagactc
     3901 ccatcgcagg gaccagagcc agtcgaacca acccagacca ccacgaccat cagccacggc
     3961 taccgctaca atgatatcgc cccgaccgca gacacaaacg gaccctacag tggctatgcg
     4021 aaggaaacag tcgagttctc cgccgccgca agcgtcgacc cagacggcat catcatcctg
     4081 tacaaatggt ccttcggaga cggcaccacc gcagaaggaa caacagcgac ccatacctac
     4141 tcccacgcag gcacctacca agtccgcctc aaagtcaccg ataacttcgg cctctcagac
     4201 accgacgtca ccaacgcgac catcaccgta cgaaacaacc caccaacacc attcatcaac
     4261 ggaccagtca acgggaccac caacaccatc tacgcctacg ccttcagcgc cactgaccaa
     4321 gaacccctca tcacctacca gatcaactgg ggcgacggca cacccaccca gaccgcaaca
     4381 cacccaagc
//
LOCUS       DATMXJ010000138        12264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_65584, whole genome shotgun sequence.
ACCESSION   DATMXJ010000138 DATMXJ010000000
VERSION     DATMXJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65584"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VN377_03700"
     CDS             complement(<1..1655)
                     /locus_tag="VN377_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27525.1"
                     /translation="MKKIIVITFLGIFIASGWTAAASEPKDSLGNTMILKNNTLSLPC
                     SSRPVILERAESIVVELKEMTSQLREPNKPILPIIVKTFQVPYGSKNIQVTCTPHDIN
                     TMALTKKIMAACIAPLSKINAIQTVKDDSIYNSTVYYPKSWYHSTIGAGSDTNRDKII
                     IVKIVCYPVRYSPANNQIEYTTSFDIALTYDEPMTIAQSNETYDLVIIAPASFKAKLQ
                     PLIDFKNAKGLKTTFKAVEDILDQYTGADPPEQVKYFIKDAYDTWGIRYVLLVGGLKS
                     HFYANDKDTPSAGWKAWWVPVRYVNIPQDADEGCLSDLYYGCLYNSTGDFDSWDSNGD
                     GIYAAWNAPGAEKDTFDLYPEVSVGRLPVTNKREVGNIVKKIITYESTSPEEKPWYKN
                     FIGIGGKTFGYYQGQPDGEYLCNLTYTYTKQAIPDLTLIPVYATNKNTSGLVPVTKDI
                     QKAISQGAGYVDFQGHGSPLAWDTIWFDGAYPNDWCGGITLYNFLKLSNDEKLPIVVV
                     GGCHNGLYNVSLLKTMRDKTGSQYFTYGIPLPVCFSWGLVVKYP"
     gene            1881..3242
                     /locus_tag="VN377_03705"
     CDS             1881..3242
                     /locus_tag="VN377_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HWR27526.1"
                     /translation="MHRLWQHLFDEDTGRGIALTTFFYNSTYKKTYESAKQVEGWSFD
                     QIQTYQWKQLSVLLHHAYAHVPYYQKLFKTLGMTPGDIKSFEDFSHLPLLGKETVQEH
                     ANEFKATNYPPSAFEETNTGGSTGFSLRFSIEKGVWFAKHLAYLRILLNRGGCDVMDN
                     SVQIIGREKPWEFRPLSRTLVLSSYQMTEQNLPLYIKKIIQRHPGYFIGYPSAMTMLA
                     TYMKQHSMEVKGVKAIYSFGETIYDWQREYLESFFHCRVHSQYGHREQCVLAGTCEKA
                     NTYHNFPDYGYVELIDRNGRPVTKEGDIGEIVATGFHTGIFPFIRYKTGDIAVCTTQQ
                     CECGRHVQLLKSVEGRIQDFMVSKTNRFVPLMGVLQLISRSSPHMKEYQLYQDHEGEL
                     ILHIVKKEGFSDTDVYQIKENIRTRLGDEFSLSIEYLDVIPRTIRGKCQFFVQKLPVK
                     YIL"
     gene            complement(3306..4865)
                     /locus_tag="VN377_03710"
     CDS             complement(3306..4865)
                     /locus_tag="VN377_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27527.1"
                     /translation="MKKNSIKVVCTLVILASLAIPTSAMIQPKKHVSLMVPLMEDFDP
                     LVDNITVTVTIKEIRALKTIDLLSDPDFYVKVRINGMVSISDVWENMKYVEDPDFSVS
                     TQVPKDDEFVNISIELWDKNNKIDLLCDISPDEGNLTQARTAELTYSIATGIWWGDDY
                     LGDPSGLGRLNGCDDNSIYQQDRDCELWFDISQNDYDGDGLPYWLETNMYNTSPLVDN
                     RGEDADQDGIPIEWEHFFGLTYFEWGHDEGYYMIYDPFVWENHTGIDDDNDGLNNIEE
                     YKTWQWGSDPFRQDIFVELDQMEKAPGNQEKTVPLESYDLIRDSHAKHNIVWHIDDGR
                     LGGGEIVPYKADLQEYDLEQFYWKYFMHQDANNWRRGVFHWGIVGYNVTQTTGFTFSS
                     RIGQMSAIDCFYLPAVFLNSQVKVIPLISSLVRKTFNSEKQKALIYAGVIMHETGHSL
                     GINCPGADCHDGLWPWQVNYWRFGPYRSVMNYRYVYSDLVDYSDGSRGKNDWNDWSTI
                     DLANFNPRPHW"
     gene            5031..5375
                     /locus_tag="VN377_03715"
     CDS             5031..5375
                     /locus_tag="VN377_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27528.1"
                     /translation="MKLLIPPEPLLLKKQSEEHKGKAWDIDSLSLWFGNQLPTYLWND
                     AGWSTPLKAEGYNWQSFLKILSLHKKEMIRWSRNALSWKEFLLKLQDTIKDPVFKTIL
                     LRKDKREATIDG"
     gene            5368..5661
                     /locus_tag="VN377_03720"
     CDS             5368..5661
                     /locus_tag="VN377_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27529.1"
                     /translation="MDKHQDGYPIVLTASRAEANEYDDNPFAAFICTFPKKLSGLALR
                     DHIENIPSNDDGTAQRTIYGLRKVESLLTKEFGEENIVVAHYDQLHRFIGKDT"
     gene            5689..6987
                     /locus_tag="VN377_03725"
     CDS             5689..6987
                     /locus_tag="VN377_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27530.1"
                     /translation="MGLAYVSTTYNSLIGFGGEALNAAEFEKLITHPAIQQYKPKIIV
                     GGAGSWQINEAGTQKKWGIDVLFQGEGEEDLISVFKKMVNNEPVPSYFVAKKPDRKKI
                     PPITHAACYGMVEITRGCGRGCQFCSPTNRTKYSFPLDYIMKEVKTNVDGGSSTIFTV
                     TEDIFLYKSKPGFIPNRDEIVKLYKTIASYPGVEHILLSHASLAPVIYDRKLMDELSP
                     ILLEKTRWTPQLNKSYPKRFVSVEVGIETGSVRLMQKYMKGKALPYSIDNWPELVVQG
                     VGIMNDYDWWPLCTIMTGQPDETEDDVRATLDLIDDLRNHHAKMFYTPVLFIPLKEAM
                     LGNRRRTNLKNLSELQWEVIARCWRNNIDFWRPDMKWMLNPIFFFSHWFYARWKHGKK
                     ATVPMMHLAGFPIAQKTNKLCTPEYCKDDASMTNGRQRSF"
     gene            complement(7003..8346)
                     /locus_tag="VN377_03730"
     CDS             complement(7003..8346)
                     /locus_tag="VN377_03730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWR27531.1"
                     /translation="MKDGESKYAYKKKSAWDKFSKQQITTAFTFAEGYKTFLNEVKTE
                     REAIHVIAEAAKKQKKKIVVNQEKSAAIIIKGKKPVRDGLRIVISHIDSPRLDLKQVP
                     LYQDSESHLALFETHYYGGIKKFQWVVIPLALHGVIVKKDGKKITFALGEDDLDPVFS
                     VPDLLPHLSHDIQDNKKIDEAIKAESLDVVIGSQPARGEFKEKIKASILEKLHAEYDI
                     TEEDFISAELEAVPAFKARDMGFDRSLIGAYGQDDRICAYAALQAILDIKNPEHTVLS
                     LFVDKEEIGSEGNASAQVSSFLRLLIKEQDANVDIDSVLVKSKVISADVSSAVTPNYT
                     DAFELKNASSLGNGVVMSKYTGHGGKYSANDASAEYIAEIRALLDKAKVPWQTGELGK
                     VDKGGGGTVAKYFSRLGMEVVDLGAPVLSMHSPYEVASKVDLYASYLAYLAFLKS"
     gene            complement(8434..9399)
                     /locus_tag="VN377_03735"
     CDS             complement(8434..9399)
                     /locus_tag="VN377_03735"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27532.1"
                     /translation="MKHSKLWLVTMIVSSLFLIATFSVQAADVTITDIIEDVSSVDYI
                     TQGTTVVTSNPYIDVANIDIVKATYSQQGTQATVTIQVRGSIENRGKIIDDYDQDSFN
                     DLNFVEYDFSLSTSEQDYTISYANQTGQISNGIDTTNLTSSDFTVAGDTLTIVFPLTS
                     ATEVYDNMSVTSMFVKMNILGDEPDLTDFVYLSDIAPNPALEIMEAYAPNIGAVGDTI
                     QFNGSIIPLTGQPPYQYHWDFGDQKTSTELNPTHAYTKTGEYTYTFTVTDQAGDTVSQ
                     TGSITISSEGGSNGSSNNQMLLFLAILAIVIVVGVVIIIWIIRRR"
     gene            complement(9500..9820)
                     /locus_tag="VN377_03740"
     CDS             complement(9500..9820)
                     /locus_tag="VN377_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27533.1"
                     /translation="MSERQRDKNSITNVAEKELDLLIRHVNILKTVRDHGPIGIIRLS
                     QITGQPQHVIRYSLRTLEHDGIIKPSAQGAIITDKIHETLGTLESTLDDMITTMSDLK
                     KKLK"
     gene            complement(10104..11411)
                     /locus_tag="VN377_03745"
     CDS             complement(10104..11411)
                     /locus_tag="VN377_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27534.1"
                     /translation="MNVLLTASGPPRHSGKRSILVSLAEQFIQRPSLTLTQLAAITPP
                     EHTITVVDENNGQVIDFKKNYDVVAISSFTKYAFRAYEIADAFRVKGSTTIIGGYHAS
                     ALPDEAKQHADAVVIGEAEEIWPSVLNDYEHGRLKPLYRQETPVDPCLIPPAKYYVRG
                     KKITAGNVQASRGCPFQCEFCSVGMVEGHRFRARPIDNVIEEIKALPTSFFSFNDSSL
                     TIDPGYTKDLFRAMRGLGKRFRCLGNINALYEDEDLIRLSKQAGCEAWHIGFESILQD
                     SLDSVKKKNSIRLYTSGIKKIKQHGVGVKGLFIFGFDNDTRSIFSDTYKAVQEWNLDG
                     ADFSILTPYPGTPLFTRLEQEGRILTKDWSLYNLGNVVFTPRYMTPQELYEGTRTIAH
                     AFHSWPSLFRMTLRNDAFDLPGVINKFIGNLWDTKIMIKEQGF"
     gene            12004..>12264
                     /locus_tag="VN377_03750"
     CDS             12004..>12264
                     /locus_tag="VN377_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27535.1"
                     /translation="MEQNLDAIQAASNVSKLIMENDRVRVIDALFKPHEKAAMHTHPD
                     HVLYIFTDGKMRLTSPKGKTQELDLKVGQTIWMDATSHAAENL"
BASE COUNT         3362 a         2661 c         2765 g         3476 t
ORIGIN      
        1 gggtatttca cgactaaccc ccaactaaaa cagactggga gaggaatccc gtacgtgaag
       61 tattgcgaac cggttttatc acgcatcgtc ttgaggagtg agacattgta aagtccgtta
      121 tgacatcctc cgacaacgac aatggggagt ttttcgtcat ttgataattt caggaagtta
      181 taaagggtga ttccaccgca ccaatcattc ggataggctc catcaaacca aatcgtgtcc
      241 catgccaggg ggctcccatg tccttggaaa tcgacgtacc ctgctccttg tgatatcgct
      301 ttttggatgt cttttgttac gggaaccaat ccgctggtgt ttttatttgt cgcatagact
      361 ggtatcagcg tcagatcagg tattgcttgt tttgtgtagg tataggtaag attacagagg
      421 tattctccgt caggttgtcc ttggtaatac ccgaaggttt tcccaccgat gccaataaag
      481 ttcttgtacc agggtttctc ctctgggctg gtgctctcat aggtgatgat tttctttacg
      541 atattgccta cttctctctt gttggtgacc ggtaatcgtc cgacgctaac ctcggggtac
      601 aggtcgaagg tatctttttc tgcgcctggt gcgttccagg ctgcgtagat tccgtctcca
      661 tttgagtccc agctgtcaaa atcaccggtt gaattgtaca agcatccgta atagaggtct
      721 gagaggcatc cttcgtctgc gtcctgtggg atattgacgt atcggacagg aacccaccat
      781 gccttccagc ctgcggatgg cgtatctttg tcgtttgcat agaaatgtga tttcaaaccc
      841 ccgacgagga gcacatagcg gattccccag gtgtcgtatg catctttaat gaaatatttt
      901 acttgctctg gcgggtctgc ccccgtgtat tggtcaagga tatcttcgac tgctttgaag
      961 gtcgtcttca gtccttttgc attcttaaaa tcgatgagcg gttggagttt tgctttaaag
     1021 gatgccgggg cgatgatgac gaggtcatag gtttcattgg actgtgctat ggtcattggt
     1081 tcatcatagg ttaatgcgat gtcaaaagaa gttgtatatt cgatttggtt gtttgccggg
     1141 gagtaccgga ccggatagca gacaattttt acaataatga ttttgtctcg attcgtgtcg
     1201 gatccagcac cgatcgtgga gtgataccag ctctttggat agtatacagt actgttgtag
     1261 atggaatcgt ctttgacagt ttgtatcgcg tttatttttg agaggggggc gatacaggct
     1321 gccatgattt tctttgtgag agccatggtg ttgatatcat gtggtgtgca ggtgacttgt
     1381 atgttttttg atccgtaggg gacctggaag gtttttacga tgatcgggag gatcggtttg
     1441 tttggttctc tgagttgtga ggtcatttcc tttagttcaa caacgatgga ttctgctcgt
     1501 tcgaggatca ctggtcggga ggaacacggt agtgataggg tattgttctt tagtatcatg
     1561 gtgtttccca gggaatcttt tggttctgat gcagctgcgg tccatccgct agcaatgaaa
     1621 atgcctagga aggtaattac gatgattttc ttcatatgtc tcctcaagtt tttatgtgca
     1681 tcctttatcg atcgtatgtg cttgatacga actattaata ttatatagta tatataaaag
     1741 tatataaata ttctgtaaat gtccccctcc tgaactactc caaataacag gaggggatac
     1801 ctatccattg tttctccttg acacagaaac tgttatctag cggataacga ctcccccaac
     1861 acaaaacaac ctgaggagtt atgcacagac tctggcaaca cctcttcgat gaagacacag
     1921 ggcgcgggat cgcactaacc acatttttct acaattcaac ctataaaaag acctacgagt
     1981 cagcaaaaca ggtagaagga tggtcttttg atcaaataca aacgtaccaa tggaagcagc
     2041 tttccgtact cctccatcat gcatacgcac atgttccata ctaccaaaaa ctcttcaaaa
     2101 cactagggat gacgccaggc gatatcaaat cattcgagga cttttcacac ctgccgctcc
     2161 ttggaaaaga aacagtccaa gaacatgcaa acgagttcaa ggcgaccaat tatccaccat
     2221 ctgcatttga ggaaacaaac acaggcggat cgactggttt ttcgctcaga ttctccatcg
     2281 aaaaaggagt atggttcgca aaacacctcg catacctcag aatactgctc aaccgcggtg
     2341 gctgcgacgt catggataac tccgttcaaa tcatcggcag ggaaaaaccc tgggagttcc
     2401 gcccgttgtc acggaccctg gtactttcct catatcaaat gacagaacaa aacctccctc
     2461 tctacataaa aaaaatcata caacggcacc caggatattt cataggatat ccatccgcga
     2521 tgacgatgct ggcgacatat atgaaacaac attccatgga agtcaaagga gtgaaagcga
     2581 tttactcgtt cggggaaacc atctatgatt ggcaacgtga atacctcgaa tcatttttcc
     2641 actgccgggt tcatagccaa tatggacacc gagaacaatg tgttcttgct ggcacctgcg
     2701 aaaaagccaa cacctatcat aacttcccag actacgggta tgtcgaactc atcgacagaa
     2761 acggacgacc agtgacaaaa gaaggtgaca tcggggagat tgtcgccacc gggtttcaca
     2821 cggggatttt cccgttcata cgatataaaa caggggatat cgctgtctgc acgactcagc
     2881 agtgtgaatg tggaaggcat gttcaactcc tcaagtcagt cgagggacga atccaagatt
     2941 tcatggtctc gaagaccaat cgattcgtgc cgctgatggg agtgctacag cttatttcaa
     3001 gatcatctcc acatatgaaa gaataccaac tctaccagga ccatgaaggc gaactgatcc
     3061 tccatatcgt aaaaaaagaa ggattctccg atactgacgt ttatcaaatc aaggaaaata
     3121 tccgcacacg actcggggat gaattttcgc tcagcatcga atatctggat gttattcctc
     3181 ggaccatccg cgggaaatgt caattttttg ttcagaaact accagtgaaa tatatcctgt
     3241 aaagaaaaaa aagaaagcag agacactctc tgtatttctt tcaatggttt tcctgatggt
     3301 gccgtttacc aatgaggcct tgggttgaaa ttggccaaat caatggttga ccaatcattc
     3361 caatcgtttt tcccacgaga cccatcagaa taatcgacaa gatcgctgta cacgtatcga
     3421 taattcatca cgcttctgta tggcccaaac cgccagtaat tcacctgcca aggccacaaa
     3481 ccatcatggc aatcagcccc ggggcagttg attcctaggg aatgaccggt ctcatgcatg
     3541 atgacaccag cgtagatcaa cgctttttgt ttctcactgt tgaaggtttt tctcaccaga
     3601 cttgaaataa gtggaataac ttttacctgg gaatttaaaa acaccgctgg gagatagaaa
     3661 caatctatgg cagacatctg accaattctt gaggagaacg taaaaccagt agtctgggtg
     3721 acattatatc cgacgatccc ccagtggaag acacctctcc gccagttatt tgcatcctga
     3781 tgcatgaagt atttccagta aaactgctca aggtcatact cctgaagatc tgctttatag
     3841 ggaacgattt cacctccacc aaggcgacca tcatcgatat gccagacgat attatgtttt
     3901 gcatgcgaat ctcgtatgag gtcatatgat tcaagtggga cggttttttc ttggttccca
     3961 ggtgcttttt ccatctgatc aagttcaacg aatatatcct gcctgaacgg atccgaaccc
     4021 cattgccatg tcttgtattc ctcgatgttg ttcaatccat cgttatcgtc gtcgattccg
     4081 gtatgattct cccagacgaa cgggtcatag atcatatagt acccttcgtc atggccccat
     4141 tcaaaatagg ttaaaccgaa gaaatgttcc cattcgatgg gaatcccatc ttggtctgca
     4201 tcctcgccgc ggttatcgac taacggactg gtgttataca tgttcgtctc aagccagtag
     4261 ggtaaaccgt ctccatcgta atcattctgt gaaatatcaa accacagttc gcagtctcga
     4321 tcctgttgat aaatgctatt gtcatcacat ccgtttaacc gccctaaccc actggggtct
     4381 ccaaggtaat catcccccca ccagataccg gtagcgatac tatatgtcaa ttctgcggtg
     4441 cgcgcctggg ttaaattccc ttcatccggg ctgatatcgc agagcaggtc aattttattg
     4501 tttttgtccc aaagttcgat tgagatgtta acgaactcat cgtccttggg gacttgagtt
     4561 gaaacagaaa aatctgggtc ctcgacgtat ttcatatttt cccaaacatc actgatagat
     4621 accataccgt ttatccttac ttttacgtaa aaatcaggat cagataataa atcgattgtt
     4681 ttcaaagcgc ggatttcctt tatggtcacg gtgaccgtaa tattgtcgac cagcgggtcg
     4741 aagtcttcca tcagcggcac catcaatgag acatgctttt ttggttgaat catcgcagac
     4801 gttggtatgg ccaacgatgc gagaataaca agcgtacata caacttttat agaattcttt
     4861 ttcatagata tccccctaag gatagtcttt tcttattcag ctatatagaa gctacttata
     4921 tataaatatt tgttacttga gggaaaaaaa agtggttcat ttttttctaa aattataaaa
     4981 actattggac attaccatag gccatctacc atgtatatgg gggatcatcg atgaagttac
     5041 tcataccacc agaaccgctc ttgttaaaga aacaatcaga agaacataaa ggaaaagcat
     5101 gggatatcga ttctctgagc ctgtggttcg gcaaccaact accaacctat ctctggaacg
     5161 acgcaggatg gtccacacca ttaaaggcag aagggtacaa ctggcaaagt ttcctaaaaa
     5221 tactctccct tcataaaaaa gagatgatcc ggtggtcccg caacgcactc tcctggaaag
     5281 aattcttgtt aaagctgcaa gatacgataa aggacccagt cttcaaaaca atcctcctga
     5341 gaaaagacaa acgggaggca actatcgatg gataaacatc aggacggtta cccgattgtc
     5401 cttactgcat cacgtgcaga ggcgaacgaa tacgacgaca atcctttcgc agcgttcatt
     5461 tgtacatttc cgaagaagct gtccgggtta gcattacggg atcatataga gaacatccct
     5521 agtaacgatg atggaactgc gcaaagaacc atctatggtc ttcgaaaagt tgaatcattg
     5581 ctgacaaagg agtttggtga agaaaacatc gttgtcgcac attacgacca attacacaga
     5641 tttatcggga aggatacgta ggttgtcggc atctcctcca tggaccccat gggactagcc
     5701 tatgtgtcca ctacttacaa ttcgctgatc gggtttggcg gggaggcgtt gaacgccgct
     5761 gaattcgaaa aactgatcac ccatcccgca atccaacaat ataaaccaaa gatcattgtt
     5821 ggtggtgcgg gttcttggca gatcaacgaa gcagggacgc aaaaaaaatg gggcatcgac
     5881 gttttgttcc aaggagaagg cgaagaagat ctcatcagtg tttttaaaaa aatggtcaac
     5941 aatgagccgg taccatcgta ttttgttgct aagaaaccgg acaggaaaaa aatcccgccc
     6001 atcacccatg cggcgtgcta cgggatggta gaaatcaccc gtggatgcgg acgaggctgt
     6061 cagttctgca gtccaacgaa ccggacaaaa tattcatttc ccctcgacta catcatgaag
     6121 gaagtaaaaa ccaacgtcga tggcggatca tcaacgattt ttacggtcac cgaggatatt
     6181 ttcttgtata aatctaaacc tggtttcatc ccgaatcgag acgaaatcgt aaaattatac
     6241 aagactatcg catcgtaccc tggtgttgaa catattctgc tttctcatgc ttctcttgca
     6301 cccgttattt atgatcgaaa acttatggat gagttatcac cgatacttct cgaaaaaaca
     6361 cgatggacac cccagttaaa taaatcctac ccgaagcggt tcgtctctgt tgaagtcggc
     6421 attgagactg gtagtgtgcg ccttatgcag aaatacatga agggaaaagc actcccttac
     6481 agtatcgata attggcctga actcgtcgta cagggcgtag gcatcatgaa cgactacgac
     6541 tggtggccgt tgtgtaccat catgacggga caacctgatg agactgagga tgatgtccgt
     6601 gcgaccctcg atcttatcga tgatctgagg aaccatcatg cgaagatgtt ctacacgcca
     6661 gtgcttttca ttccgttaaa agaagcgatg ttaggaaacc gtcgtcggac caatctgaaa
     6721 aacctctcag agctgcaatg ggaggtcatc gcccgatgct ggaggaacaa tatcgatttc
     6781 tggaggcctg acatgaaatg gatgctgaat ccgatatttt tcttttctca ctggttttac
     6841 gcacggtgga aacacgggaa gaaagcaacg gtgcctatga tgcatcttgc tggatttccc
     6901 atcgcccaaa aaacaaataa actctgtaca ccagaatatt gtaaggatga tgcctcaatg
     6961 acgaatgggc gacaacgatc gttctaacca aaaacaatac agttagcttt ttaagaaggc
     7021 gaggtacgcg agatacgagg cataaagatc gactttactg gcgacctcat agggtgaatg
     7081 catggatagc acgggggcac caagatcaac gacttccata cctaaccgtg agaaatattt
     7141 tgcaactgtt cctccaccgc ccttatcgac ctttccaagt tccccggtct gccatgggac
     7201 cttcgccttg tcaagcaatg ctctgatctc agcaatatat tctgctgacg catcattggc
     7261 tgagtatttc ccaccatggc cggtatattt ggacatcacc acaccattcc ctagcgatga
     7321 tgcgtttttc agttcaaagg catcagtgta attcggggtg acagctgagg agacatccgc
     7381 agagatcacc tttgatttga ccaggacact atcgatatcg acatttgcat cctgctcctt
     7441 gataagtaac cggagaaaag aagaaacctg tgcagaggcg tttccttcgc tgcctatttc
     7501 ctctttatcg acgaaaaggg acaaaaccgt atgttccggg ttttttatat ccaagatcgc
     7561 ctgtaacgct gcataggcac agatacgatc atcctgtcca tacgcaccaa tcaatgatcg
     7621 gtcgaatccc atatcacgcg ccttgaaggc aggaaccgcc tcaagttccg cggagataaa
     7681 atcctcttcg gtgatatcgt actcagcatg gagtttttca aggattgatg ctttgatttt
     7741 ttctttgaac tcgcctcttg caggttgact tcctatcacg acatctaaac tttctgcttt
     7801 gattgcttca tcgatttttt tattatcctg gatatcatgg gacagatgag gcagtaaatc
     7861 tggtactgaa aagacggggt caaggtcatc ctcccccaag gcgaacgtga tttttttgcc
     7921 gtcttttttc acaatcaccc catgtaatgc gagtgggatg acgacccatt ggaatttctt
     7981 aatacctcca tagtaatgtg tttcgaacag agcaagatga ctctctgagt cttgatagag
     8041 gggtacttgt ttgagatcaa gtcgaggaga atcgatgtgg gaaatgacga tgcgcaggcc
     8101 gtcgcgaaca ggtttttttc ctttgatgat gatcgctgct gatttttctt ggttgacgac
     8161 gatttttttc ttttgttttt ttgctgcctc agcaatcaca tggattgcct ctcgttctgt
     8221 ttttacttcg ttgaggaacg tcttgtagcc ttctgcgaat gtaaaggcag tggtgatttg
     8281 ttgtttagaa aatttgtccc aggcagattt cttcttatat gcgtatttgc tttcgccatc
     8341 cttcatggac tagacctctc ggtatgaaag tcatcttttc agaccataaa aagataactc
     8401 aagaaaaaaa agaaaaaagg atggttgagg gggttatcgt cgtcggatga tccagatgat
     8461 gatcacgaca cctaccacaa taacgatagc caatatcgcc aggaacagga gcatctgatt
     8521 gtttgaagag ccgtttgagc ctccctccga agagatcgtg atactgccgg tctgacttac
     8581 ggtatcgcct gcttgatcgg ttacggtaaa ggtataggtg tactcgcctg tttttgtata
     8641 ggcatgggtg ggattcaatt ctgttgacgt cttttgatcc ccgaaatccc aatgatattg
     8701 atagggaggt tgaccggtga gaggtatgat gctcccgtta aattgtatcg tatcacctac
     8761 tgcaccaatg tttggtgcgt acgcttccat tatctccagt gcgggatttg gtgcgatatc
     8821 tgacaggtag acgaagtcgg tgaggtctgg ttcgtctcct aatatattca ttttcacgaa
     8881 catggacgtg acactcatat tatcatagac ttctgtggca cttgtcaatg ggaagacaat
     8941 ggtaagagtg tcaccagcaa cggtgaaatc agaagaggtc agattcgttg tgtcaatgcc
     9001 attgctgatt tgacctgttt ggttggcgta actaatggta taatcttgct ctgaagtact
     9061 tagagaaaaa tcatattcta caaaattaag gtcattaaaa gaatcctgat cgtaatcgtc
     9121 gatgattttt cccctgttct ctatagaacc tcttacttgt atggttacgg ttgcttgtgt
     9181 tccttgctgg gaataggttg ctttaacaat atcaatattt gcaacatcaa tatatggatt
     9241 ggaggtaact acggtggttc cttgtgtaat ataatcaacg gagcttacat cttcaatgat
     9301 atctgttatg gttacatctg cagcctggac agaaaatgta gcgatgagga agaggctgct
     9361 tactatcatc gttacgagcc ataatttgct gtgtttcatt tttatgcacc tcgtttcggt
     9421 tgagaaggac tatttagtta tcctatttaa aaattttgga gcactttcgt atctacgttt
     9481 ttcattgaaa aaatctcttt tatttcaatt tctttttcaa atcagacatc gtagttatca
     9541 tgtcgtcgag ggtagattca agggtcccca gtgtctcatg gattttatcg gtgatgatgg
     9601 cgccttgagc ggatggtttt atgataccat catgttccaa cgttcgaagt gagtatctga
     9661 tcacgtgttg gggttggccg gttatctgtg agagtctgat gatgccgata ggcccgtgat
     9721 cgcgaacggt ttttaagata ttgacgtgtc tgatgagaag gtctagttct ttttctgcta
     9781 cattggtaat agaattttta tctcgttgtc tttccgacaa tacactacct ccctggttaa
     9841 ttgttaacaa ttagtatgca atggagatat ataatcttta cgagcctcga ggttgttccg
     9901 aaagttacta tcaaatatca actggatgat aaaagaaggg ttgatatgag tctttctttt
     9961 atctatttaa acagaattaa gcatttggat aaaaaagtgc aggagaaggg atctgttctt
    10021 catcgggaac aggaagtcat cttcttttac aattttgctt ttggataaaa aagcaccatg
    10081 ctagtaatcc aaaggatgat tcgtcagaaa ccctgctctt tgatcatgat tttcgtgtcc
    10141 cagagattac caatgaattt gttaattacg ccagggaggt caaaagcatc gttccgtaag
    10201 gtcatccgga acagggacgg ccatgaatga aacgcgtgag caatggttct cgtgccttca
    10261 tacaattcct gcggcgtcat gtatcgaggt gtgaagacga cattcccaag gttgtacaac
    10321 gaccagtctt tcgtaagaat gcgtccttcc tgctcaagcc ttgtgaacaa aggtgtgcca
    10381 gggtagggtg tgaggatgct gaaatctgcc ccgtcgagat tccattcctg aaccgctttg
    10441 taggtgtcag agaagatgct gcgtgtatca ttgtcaaaac cgaagatgaa gaggcctttg
    10501 acaccgacgc catgttgctt tattttcttt atcccggagg tgtagagacg tatgctgttt
    10561 tttttcttga ctgaatcaag actgtcctgg aggatggact cgaagccgat atgccatgcc
    10621 tcacatcctg cctgtttcga cagtcggatc agatcctcat cctcatagag ggcgttaata
    10681 ttgcctaagc atcggaacct tttgccaagc cctctcatcg ctcggaacag gtccttcgtg
    10741 taaccggggt cgatggtcag ggaggaatcg ttaaaggaaa aaaaagaggt tggtaaggcc
    10801 ttgatttcct cgatgacgtt gtcgatgggt cgtgccctga atcgatgacc ttcgaccatg
    10861 ccgacggaac agaattcaca ttggaacgga catccgcggc tagcctgtac attacccgcc
    10921 gttatctttt tccccctgac gtaatatttt gctggcggaa tcaggcacgg atcaacgggt
    10981 gtctcctgtc gatacagggg tttaagtctg ccgtgctcat aatcgttaag gaccgaaggc
    11041 cagatctctt cagcttcacc gatgaccacg gcatctgcat gctgtttagc ttcatccggt
    11101 aaggctgagg cgtgataccc gccgatgatg gtcgttgatc cttttaccct gaatgcgtcc
    11161 gcgatctcat aggctcgaaa ggcgtatttg gtgaacgatg agatggctac gacgtcatag
    11221 ttcttcttaa aatcaatgac ttgaccattg ttctcatcaa cgaccgtgat ggtatgttct
    11281 gggggcgtga tggcagccag ctgagtcaaa gtcagagacg ggcgttgaat gaactgttca
    11341 gcgagtgaaa cgaggatcga tcgtttccct gaatgtcgcg gcggtcctga tgccgtgagg
    11401 agtacgttca tatcatcatc ctgaaatatg tttgattgac cagatcatga cccgatagag
    11461 attgtcatca gcagttctag gaggaaactg cagattcaag ttcgaaggtt aaaggaggtt
    11521 attttgatat gtacaggaaa tatacctgta agtaagtatt taaaattatt tgccacttaa
    11581 gcaaactggt tttggaaagt aatcattcta ttgtgcgcaa caggtcacgc atctgtgcaa
    11641 tttgctgaat tggcagctaa taattcaatt tatggatacg tgcttccgtc aggattacgt
    11701 accaaaggtt caattccctc gggagaggga aagtgaaggt ctggcataaa atctcctata
    11761 gtgatcctgt ccagagataa acacgaatat atcggtaaat caatacagat tgatagtgca
    11821 ggggaaggga tttgaacatc aggactattt atgctctcct gttgcttatg gtgtgaaata
    11881 ggattataga ggattattgt ttttaaatcg ttttaaaggt gtttttaata taatttatta
    11941 taatatcaaa caatctatta actaaatcat aaagaaacat aaaaagaaag gagatgaaaa
    12001 aatatggaac aaaatttaga tgcaatacaa gcagcatcaa atgtatcaaa actaatcatg
    12061 gaaaacgacc gcgtgcgcgt gatcgacgct ttatttaaac cccatgaaaa agcagctatg
    12121 catacgcacc ctgaccacgt attatatatt ttcaccgatg ggaaaatgag gctaacgtca
    12181 cctaaaggta aaacacaaga attagatctt aaagtcgggc agacaatctg gatggatgcc
    12241 acatcccatg cagcagaaaa tttg
//
LOCUS       DATMXJ010000139        16647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55063, whole genome shotgun sequence.
ACCESSION   DATMXJ010000139 DATMXJ010000000
VERSION     DATMXJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55063"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..671)
                     /locus_tag="VN377_03755"
     CDS             complement(225..671)
                     /locus_tag="VN377_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27536.1"
                     /translation="MNAIDTKIKNLVYFLNTLPTVKTIGSCQEHDDGGETGNFLVPYI
                     SFTCNDRRVLGFLASLRFAYEDPSEILPKYRKNQPWLRADWCIYVITGEDPEYSPEEL
                     KADNRQEDYVFYVLQPNRYSYDKPSDIYVDFSVILKYYQWKWKKMK"
     gene            complement(927..7298)
                     /locus_tag="VN377_03760"
     CDS             complement(927..7298)
                     /locus_tag="VN377_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWR27537.1"
                     /translation="MNLDYEYVWEQVQRFGHVIYNVNNWPNDIKKGREWATAGEEYTI
                     DNILKDAMDGPDKPCGLSEYAEIPIGYIDQIPFRSRQYSSKIVISDYSLTLNYDGEPS
                     LELSTDELFPMGIGNLNFIQGICTEEDLDDTFEFNDAELIPIDIHKSVIEQVIDLDFY
                     TVPCELLNEYTTILGPVVYLETNDTVPENQDCVFILNEEPSSEEKLDNLSDALGCILI
                     EDSSKGYTFNNSSNYDFTITEINETDSNFSSVIEEIQKGSVFVADNIQDGETIVFSNC
                     SGQSCCSGSDKVVFFTLESEDDTRLITFWLNLWIWGHCRKCKGAIMINSDEHTHNMMH
                     TVRGWKWFAKEIILPGPYSNRFPLPIFSVNGSMLDDIQNAITVDGFITQEYEKQTEDN
                     PVGVISHNVVAYRNITQSPNNAIAVLSNRMDSWWGECPGDAGTSGAILLGIAKYMEDN
                     DITPKYDLTFLFTTGEEYGLRGAQHYVDSHPEDEYNYKYWIGFEQLGFNYPGGEEHNL
                     NMLSYNADLLPILSEIQKQTNYEARMHNQSYQYKDPDEPTTGFSGAEDYVWKDRCDTI
                     LIEKHDNWSGHHQVGTNYQEGDSLQYINRTDVNVTFELAWNMTKYFVVNPNCQFDGTI
                     TYTRTDSPNDEDEEFDTIEATMPIQSILPQDKIRIKAELRRSFFPYNIEHSIIQDYIV
                     TDETSNYKINITLPPHKPEGNYTLILYLYNSTGRINQILSISPNGPNDTTNSDFLFLY
                     PRGNQAPTTPTQPDGPISVKRWQQAYWGSMSPDPNSDQIGQKWYWNYDTSPNIHINTG
                     LYDTDGFYLPHRYSILGTHRIQVKAYDEYSTLLHPYETDFSEPLDVEVTPWCDIIEQQ
                     QTNQEGLIHVVEGLPFQFYGELSGGENNDFEWNFNDRTRCSTEQNPEHIYSDSGNYNV
                     TFNVTDETTQLIGRTSVQVRVSDLDSNFNMSYYHGAAPETIITFCNTSKAKTGNSITN
                     VTWDFDDGTISYANIVNHSFETDGDYNVTLTVKDSANNTDIDYAILHITSNPEPPEIP
                     YVQSPGVLPPDSEATILAEIIPTDRNLSSVTVNITTPNGTVGNYTMTEIIENIYIYTL
                     DNTSQNGQFNFTVWATDIENNTNSTTGNFSMIVPLLCFETPTPSNNAIRNHNWINVNV
                     TVLDPLNTSAFIDWNRSLKGYWPMDTYNTTGVNDNSTYENFGTFENGINSSNIKTGKY
                     GKGFEFDGIDDYVDLGTNSSLDLGTGDFTFMVWEKSHNPFYLKKAMILSNSPVCEDWQ
                     GYGFGVANTAYLFASQSSGRNVTVEGTIDVTDNTWHHIAYVRDGGEYSIYVDGTFDTH
                     GLVIHDYNITNSQSTYLAYNGRTSNWCYFDGMLDEPQLYNRALSREEINASYNNGLYA
                     LYRNFTGLADNTYSYSAHAIDTSGNMSETETRTVTIDLDPLITNVNASPHTVGFGYNV
                     TISANITDPGSGVNTVNVNVTCPDQSHGNFTMSHATGNTYQYIFTDTWLTGQYNYTIW
                     ATDNSNNSNSSSEHHFHVSATATISIATLKDSYSGTQYINITDPPNPSENLTVVDRSL
                     TWNTYYNASSGENILETYQGPVNYQEDNGTWTPINNSFYPLASNHPAYVYGYRTGNDH
                     GLYGVYFKSNAQQEWPIAFTFNRSDDPTIHAIRSKLVGVGYVDPQSNWAYQYLQNVQS
                     SQGQTNGYSITYPSVFIGTDVTWSYGNTGLKEEITLSNATKTVLQNHPPSQYGLNDTS
                     SYLVFITKLDYQNLNLYNDSGLLDGNVTISDIGVEFKDALGQFKCALPLGEAYEPNNE
                     LIRQKLTYRIVHLNGNTYLLSGLKVSDLNAMAFPVVIDPTLSVYSLTNDGYIYNSSSN
                     YNTAWTASTGTISSSATYLSIGQSKVASFPVDYRVYRSFLPFNTSSLPSNAFIDKATL
                     SVYKKDDYSTTDFTITIQNGQTTYPHNPLQAGDYAKSHYSGNGGSLNTINFVNGRNNI
                     TLADLTWINITGITKLCLRSSRDINGNTPSGSEYVNVYSANAMDPNPDTSYKPKLIIA
                     YRNQSKIKNTGSTNINGYLLIQVQFYNTSQAKWVLDNDIVNETSPRIITSGNQLALDT
                     IFNGHVRASDLTHGTGTYRVYATFRDPDGNILKTNDGTELKAWWQFNKT"
     gene            7654..9540
                     /locus_tag="VN377_03765"
     CDS             7654..9540
                     /locus_tag="VN377_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27538.1"
                     /translation="MKSAWWKLFIILIVVIMVCPVANSLVFGEINKNSIVQQSLIIPE
                     YISDWYIDKSSIRQDHEPFSLDLRQNQSIVKKAQYHTQGDGDKKGPMDSPWPMFGHDA
                     VHTCRSPYSTENNTGVEIWRVRSDKMGSVWGSALTDNNGLIYFGTKASDDSLYALYPN
                     GTRKWKFPATALIWGTPAISEEGTIYFTTWGRYFFAVNPDGTERWRFNTGSISVSSVT
                     IAEDGALYFGDDNNIIWALNPDGTEKWRYTTGYIVMGSPAISIQDGTIYIGSGDYYLY
                     ALYPNGTLRWRFATGDYIKGSASIAPDGTIYIPSFDEYLYALYPNGTKKWQVSTGDSL
                     AAAGVAIGKLGTIYVGTEMLRAYNPDGTLKWITDVQGYMEGTVPAVSADGTIYVSAGN
                     SLVAVNPDGTEKWRIALSDEQIRSSPSIGPDDRVYVGSTYSDYGYLHALGLGPLYAEA
                     YGPYSGVVSESIQFIGDAFGGTPPYQYHWDFGDGTTSEEQNPAHSFADIRTFLAMFSV
                     TDSEGNTSEDTAQVTITAPAPTVTIVKPVNGIYIGDSRILPFFRPFIIGQITIQVDAT
                     QEPYGIDHVEFYIDGEIRATVTEIPYQWTWNTRTFFKHTINVIAFDTIGKSTEKSIDV
                     SKFF"
     gene            complement(9635..9988)
                     /locus_tag="VN377_03770"
     CDS             complement(9635..9988)
                     /locus_tag="VN377_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27539.1"
                     /translation="MPKLCITYRNQSKIKNTGSTDIRGYLLIQVQYYDTGRGTAPRWI
                     VDNDTINETIPRTITSGDQLALDTIFNGLVRASDLTHGTGTYRVYAAFRDPEGNILRT
                     NDDVNLEAWWQFNKT"
     gene            10427..10882
                     /locus_tag="VN377_03775"
     CDS             10427..10882
                     /locus_tag="VN377_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWR27540.1"
                     /translation="MARIHARTKGKSGSTHPMREKHPEWSSLNPREIEGRILELAKAE
                     KSTSEIGMILRDQYAVPDVKIATGKTITQILEQNKITQEIPEDLQNLIRTALKLKKHI
                     DQHKKDFKTKRSLSLTESKIRRLMKYYRREQRLPVGWKYSLDQAKLMFE"
     gene            10942..12288
                     /locus_tag="VN377_03780"
     CDS             10942..12288
                     /locus_tag="VN377_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWR27541.1"
                     /translation="MAEEISQACQDAASMLQSIPSSRRIRVVSHYDADGIAAAGVLCT
                     AVYRAGYEFHASLMRNPFTKGFDRLKNEHNDLIIFSDMGSGQIETIESLGCKVIVLDH
                     HQYLTTQTSKNIIQINANLFGADGNYEVSGATLSYLFATALDPSNEDLAPLALAGATG
                     DKQFIGGIRGLNRTILDNALQKGFLTEQTSIKLYGETIADALYYSVDPYYPGLSGKHE
                     KIETMLEQLHIKKTASIQEIPLETMAKIQSYLLFALIKAGCQQNIVDMTIRKRYIASQ
                     GWELERFADLLDACGKNGYRSLGLSLCLGDGTTWNEAVQVEKDYKQKLLDGLQGLEHG
                     GVQETDGMRYFFSDNSSLGGVIAGIAMNYVLDEKKPLFSLTRKETDDEVHISCRGNQQ
                     LVRQGLDLGVAMKTVAGDLGGYGGGHKIAAGATISFGKEKEFLEQVDRLLVQQMQR"
     gene            12290..12511
                     /locus_tag="VN377_03785"
     CDS             12290..12511
                     /locus_tag="VN377_03785"
                     /inference="COORDINATES: protein motif:HMM:NF011470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HWR27542.1"
                     /translation="MKASCTIILEFESAEKAKKVLRSIQVDDQGFVRSKVTDKTLEAV
                     VESSSVASLLHTLDDYLACVSVAADIVKK"
     gene            12665..14281
                     /locus_tag="VN377_03790"
     CDS             12665..14281
                     /locus_tag="VN377_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWR27543.1"
                     /translation="MKKKVVGCVLFLLLIGTTVPLISSSNSDIHPVLADEITLAEDCE
                     CESNQVLTYQITVNDTVGQSPKPTISHDLPPYFSWKDVDGVDWTTTAKDQGDCGSCWD
                     FAAMGALESIIQIREGCAGLNLDLSEQYVLSCLHAAGSCNGGWAYNAYKYIKSNTSSG
                     NYCNGIIPEFCFPYQVNDDVPCSNASPDWSNFLIPLSTYGRWVPDGSVEDRNAIKTQI
                     MESGPVVATMLFTYYAHGSDNLEEWGYTHHDSTDYYAYPGPMQGTNHQVVLVGWNDDI
                     SIQNGGYWIVKNSISEEWGYDGFFNLEYGSLNIDKTDINWVDYNPENYSNWVPVARIN
                     TSALEQMVFDGSESFDHEGAIVSYAWDFGDGSTATGMTTTHTYDAAGIYLVTLTVSDN
                     ASNTDDETAWVFINTENHPPKTPWLLGRRTGENDTAYKYTFWATDPDGDDLYYYLNWG
                     DTYWDGGAVGWIGPYKSGEKVTLEKTWAEKGNYTVRVKASDRYDAKSNWATLKVTMPL
                     SYDLPFYWFLDQLLERFPHAFPILRYLRSG"
     gene            14448..16034
                     /locus_tag="VN377_03795"
     CDS             14448..16034
                     /locus_tag="VN377_03795"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HWR27544.1"
                     /translation="MNRKGLAVGLILLLIGTSMPITAQNAKKASESTSRGNWLYVGGS
                     GPDLRKAYSYNSLKTTMESPLLQNDNWTQIQKLISLDGKPHDAFGYSVSVDGNTALVG
                     AWFDNSTQDLSGSVYVFIHSSRLWTLQQKIFAPDITDFGLSVSVSGDTALIGAGGAAY
                     IYARNGTQWYLVTKLTSSRISFGREVSLSGDTAIIGEDFGEKAYVFRCVNSTWNQEAI
                     LTASDPEPNDFFGTDVSIDGDTALVSSLQDEDNGVGSGSAYVFVRNGTIWTQQTKLLP
                     SDGKPMDWFGWSVDIKGDTAFIGAPTYWYDDPGFVYVFTRNGTLWTQQQKLLSSDGVG
                     GDRFGCSISLNGNLALIGAYFHDHMGYDSGSAYIFAFDGEQWKEEVELLASDGYEKNW
                     FGFSVSLFNGTAFIGAPYEMVSGFNPGFTYVFSEDSWNPQVKIEIQGGLGLTTIITNL
                     DLTENLNDIQWHCSAIGGLLWKINYEKNGIVNQIKPGESIKVRSGLIFGLGPVFISFN
                     VADAENTIKAKQIIFYTVSP"
     gene            16221..>16647
                     /locus_tag="VN377_03800"
     CDS             16221..>16647
                     /locus_tag="VN377_03800"
                     /inference="COORDINATES: protein motif:HMM:NF036884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27545.1"
                     /translation="MMRFELQARFTLSSDVTKLTKEFDKFIAETNETILKKGPEKLAV
                     IEKYNIEKTTLSLSISSEGTLRPHNALLQIKNALTKELGKTHHVGVRGITIEKYTISF
                     DLPREPLKDISIPFAEVTIKGKQATMLLSDVSEEFLRRNY"
BASE COUNT         4565 a         3354 c         3810 g         4918 t
ORIGIN      
        1 cttgctatct gagcttttga cttaccattc aatgcttgtt tcctaatggt tgcgatggtt
       61 tctttcgata gatgttttcc ttgcgtcatc actaccgagt gtaaaaactc atataaaaag
      121 atatggaaaa tcaggtatat tacctgattt ccattttaaa gtttttttat aatgggtctg
      181 ccaaggaacg aaaagaccag ggttatatgg atggaacccc aatttcattt cattttcttc
      241 catttccact gataatactt tagaatcact gaaaaatcaa catagatatc agagggctta
      301 tcataagaat atctgtttgg ttgtaataca taaaagacat aatcttcctg tcgattatct
      361 gctttaagtt cctcggggga atattctggg tcttctccag tgattacata aatacaccaa
      421 tcagcgcgta accatggttg attcttccta tattttggta atatttcaga cggatcttca
      481 taagcaaatc gaagactggc taagaacccc aatactcttc ggtcattaca agtaaatgaa
      541 atatacggga cgaggaaatt tcctgtttct cctccatcat cgtgttcttg acaacttcct
      601 attgttttaa ctgttggaag ggtattaaga aaatacacaa gattttttat cttagtatct
      661 atcgcgttca ttttattact ccaattaatt catcattacc gtgatacctt gctcttttta
      721 agaaagacaa gatgaactga attatgagaa cggagaggaa tggtgtgctg aataaaaatt
      781 agtatggttt tttccagagc ggttctatat aatatcatga agtagtctag cacttgttat
      841 tcaatatctg catctattga aaaaaatgat agaatgtcag atatgaacct acctgacttc
      901 tattttatga tggtttagtt ttttcgttag gttttgttga actgccacca agcctttaac
      961 tcagttccat catttgtttt cagaatgttc ccatcgggat ctctgaacgt agcatacacc
     1021 cggtaggtgc ctgtgccatg ggtcagatcc gaggctcgga catgcccgtt gaagatcgta
     1081 tcgagcgcaa gctggttgcc gctggtgata atccgcggag atgtctcatt gactatatcg
     1141 ttatctagta cccatttcgc ttgtgaggtg ttgtagaact gaacctggat caataggtat
     1201 ccgttgatgt ttgtcgagcc ggtgttcttg atcttcgatt ggttccgata tgcgattatc
     1261 agcttcggtt tatacgaggt atcaggattt ggatccattg cgttggctga atacacgttg
     1321 acatactcgc tccccgaagg ggtatttcca ttgatgtctc tgctactgcg cagacagagt
     1381 ttggtgatcc ctgttatatt aatccaggta agatctgcga gtgtgatgtt attccttccg
     1441 tttacaaaat taatagtatt gaggctaccg ccgttccctg aatagtgact cttggcgtaa
     1501 tcaccggcct gaagcgggtt gtgtgggtag gtggtttgtc cgttctgaat ggtaatggtg
     1561 aaatccgttg tcgagtaatc atccttcttg tagacagaga gggtagcttt atcaataaat
     1621 gcattggatg gaagggaaga tgtattgaag ggaagaaaac ttcggtagac ccggtaatct
     1681 actgggaaag atgctacttt actttgaccg attgatagat aggttgcaga gctagagatc
     1741 gtgccagtcg atgctgtcca tgcggtattg taattcgaac ttgagttgta gatatatcca
     1801 tcgttcgtca gtgaatacac cgacagggtt ggatcaatga ccacagggaa cgccatcgcg
     1861 ttcagatcag agaccttgag tcctgaaagg agatacgtgt tgccgttcag atgcacgata
     1921 cgatacgtca gtttctgacg tattaactca ttgtttggtt cgtaagcctc gccaagcggc
     1981 aaggcacact tgaattgccc gagcgcatcc ttgaattcaa cccctatatc agagatggtg
     2041 acgtttccat caagcaatcc tgaatcattg tagaggttga ggttttggta gtcgagtttg
     2101 gtgatgaaca cgagatagga gcttgtatca ttgagtccat attgtgatgg tggatggttc
     2161 tgtaacaccg tctttgtcgc gttactcaga gtgatttctt cttttaatcc ggtgttaccg
     2221 taactccagg tgacgtcagt accaatgaag acacttgggt aggtgataga atatccgttg
     2281 gtctgtccct ggctgctctg aacgttctgg agatattggt atgcccagtt gctctgcgga
     2341 tcaacataac cgactccaac aagcttgctc ctgatagcat ggatggttgg gtcgtctgat
     2401 cggttgaagg tgaatgcgat tggccattcc tgttgcgcat tggatttgaa atacacacca
     2461 tataatccat gatcgttccc ggtacgatat ccgtagacgt atgctggatg gttggatgcg
     2521 agcggataaa atgagttgtt gattggcgtc caggttccat tatcctcttg ataattcact
     2581 ggtccctggt atgtctctag gatgttttca ccggaggatg cgttgtagta ggtgttccag
     2641 gtgagactac gatcaaccac ggtgaggttc tcagatggat ttggtgggtc ggtgatgttg
     2701 atgtattgag taccactgta gctgtccttc agggttgcga tgctgatagt tgctgttgct
     2761 gagacatgga agtgatgttc gctgctgctg ttgctgttat tggagttatc cgttgcccag
     2821 atggtgtagt tgtactgccc ggttagccac gtatctgtaa agatgtattg gtaggtgttc
     2881 cccgttgcat gcgacatcgt aaagttccca tgcgattgat caggacaggt gacattcacg
     2941 ttcacggtat tgacaccgct tccagggtca gttatgtttg ctgaaatagt cacgttgtac
     3001 ccgaatccca cggtatgcgg cgatgcgttg acattggtga tcaatgggtc gagatcaatg
     3061 gtgaccgtcc gtgtctcagt ttcactcata tttcccgagg tatcgatcgc atgggcagag
     3121 tatgagtacg tgttgtcagc taaacctgtg aaattacgat acaatgcata gagtccgttg
     3181 ttgtaggagg cgttgatctc ctcgcgggaa agtgcccggt tgtagagctg gggttcatca
     3241 agcatcccat cgaaatagca ccagtttgat gtacgaccat tataggcaag ataagtgctt
     3301 tgcgagttcg taatgttata gtcatggatg accaacccat gggtatcaaa cgtgccgtca
     3361 acatatattg aatattctcc tccatcacgc acataagcga tatgatgcca ggtgttatcg
     3421 gtcacatcaa tagttccctc gaccgtcacg ttacgtcctg atgattgaga agcaaaaagg
     3481 tacgcagtgt tggcgacgcc gaaaccatat ccttgccaat cctcgcatac tggactgttg
     3541 gaaagaatca tcgctttctt caagtaaaag gggttgtggc ttttctccca gaccatgaat
     3601 gtgaaatcac cagtacctag atccaaacta ctgttggtgc caaggtccac gtaatcatcg
     3661 ataccgtcga actcaaatcc tttcccgtat ttccctgttt tgatgttcga ggagtttata
     3721 ccattttcga atgtcccgaa gttctcatag gtagaattgt cattgacccc ggtggtgttg
     3781 taggtgtcca tgggccagta tcctttgagg ctacgattcc agtcgatgaa cgcagaggtg
     3841 ttcaaaggat caagaaccgt gacattgaca ttgatccagt tgtgattcct tatcgcgtta
     3901 ttggagggag ttggcgtttc aaagcaaagc aaggggacga tcatggaaaa gttcccagtg
     3961 gtgctgttcg tgttattttc aatgtcggtc gcccagactg tgaagttgaa ttgtccgttc
     4021 tgcgaggtgt tatccagggt gtagatatag atgttctcaa tgatttctgt catcgtatag
     4081 ttcccgacgg tgccgttcgg ggtggtgatg ttcacggtga cactggaaag gttccgatct
     4141 gtcggtatga tttcagcaag gattgttgcc tctgaatcgg gaggaagaac tcctggactt
     4201 tgcacgtacg gaatctcagg tggttctgga ttggaggtga tatgcaggat ggcataatcg
     4261 atatcagtgt tgttcgcgct gtccttcact gttaacgtga cgttatagtc accatctgtt
     4321 tcaaaggaat gattgacgat atttgcgtag gaaatggtac catcatcgaa atcccaggtg
     4381 acattagtga tactgttacc ggttttggct ttggaggtat tacagaatgt tatgatggtt
     4441 tctggtgctg caccatggta atagctcatg ttgaaattgg agtcaaggtc ggagactcgc
     4501 acttggacag aggttcttcc aatcagttgt gtggtttcat cggtgacgtt aaaggtgaca
     4561 ttgtaattcc ctgaatcgct atagatatgc tccgggttct gctcggtgct gcatctggta
     4621 cggtcattga aattccattc aaaatcgttg ttttcacccc ctgataattc tccatagaat
     4681 tggaatggga gtccttctac gacatggatg agtccttctt gattggtttg ttgttgttca
     4741 ataatatcac accagggggt gacttctacg tctaatggtt ctgaaaaatc agtttcatag
     4801 gggtgaagga gggttgaata ttcatcatag gcttttactt gtattcgatg tgttcctaaa
     4861 atagaatatc tgtgaggtaa atagaaacca tctgtatcat agagtccggt atttatatgg
     4921 atgtttgggg atgtatcata attccagtac catttctggc ctatctgatc gctgttagga
     4981 tctggagaca tggaccccca ataggcctgt tgccatcgtt taactgatat tggtccatcg
     5041 ggttgagtcg gggtggttgg tgcttggttt ccccggggat acaggaacag aaaatctgag
     5101 tttgttgtat cattcggacc attcggtgat attgaaagaa tttgattgat ccgccctgtt
     5161 gaattatata aatagaggat aagagtataa tttccttcag gtttatgtgg tggtagagtt
     5221 atgttaattt tataatttga tgtttcgtct gtgacgatat aatcttgaat gatagaatgc
     5281 tcaatgttat agggaaagaa acttcgtcgt aattctgctt ttatgcgtat cttgtcctgg
     5341 ggcaatattg attgtatcgg catggttgct tctattgtgt caaattcttc gtcctcatca
     5401 tttggagaat ctgttcgtgt ataagttatc gttccatcaa attgacaatt gggatttaca
     5461 acgaaatatt tcgtcatatt ccacgctagc tcaaaggtca cattgacatc cgttcggttg
     5521 atatattgaa gagaatcacc ctcttgataa ttcgtaccta cttgatgatg tccgctccaa
     5581 ttatcatgtt tttcgattaa tattgtatca cagcgatctt tccagacata gtcttccgct
     5641 ccactgaaac cagttgttgg ctcatcaggg tctttgtatt gataagattg gttgtgcatt
     5701 cttgcttcgt aatttgtctg tttttggatt tcactcagaa tgggaagtaa gtcggcgttg
     5761 tatgataaca tatttagatt atgttcttca ccacctggat aattaaaacc aagctgttca
     5821 aaacctatcc aatatttata attatattca tcttcaggat gactgtcgac gtaatgctgt
     5881 gctcctcgca gcccatactc ctcaccagtg gtgaatagga aggtgaggtc gtatttaggg
     5941 gtgatgtcat tgtcttccat atattttgca ataccaagga gaattgctcc agaggtacca
     6001 gcatccccgg ggcattcacc ccaccaacta tccatgcgat tacttagcac ggctatggcg
     6061 ttattcgggc tttgggtaat attccgatag gcgacaacat tatggctaat cactccaaca
     6121 ggattatctt cggtttgttt ttcatattcc tgagttatga agccatcaac agtgatagca
     6181 ttctgtatgt catcaagcat tgagccattc actgaaaata tcggaagtgg gaatctattt
     6241 gaataaggac caggtaagat aatttcctta gcgaaccatt tccaaccccg tacagtatgc
     6301 atcatattat gagtatgttc atcagaatta atcataatag ctcctttgca ttttctacag
     6361 tgaccccaaa tccaaaggtt caaccaaaaa gtgattaatc ttgtatcatc ttcggattca
     6421 agagtaaaaa atactacctt gtcagaacca gaacaacaag attgaccact acagttggaa
     6481 aacactattg tttcgccatc ttgtatatta tcagcgacaa ataccgagcc tttttgtatc
     6541 tcttcgatga ctgaagaaaa attactatct gtttcattga tttctgtaat cgtaaaatcg
     6601 taatttgagc tattattgaa tgtataacct tttgagctat cttctatgag gatgcatcca
     6661 agtgcgtcgg agagattatc aagtttctct tcagaggatg gttcttcatt taaaataaaa
     6721 acacaatcct gattctcagg aactgtatca tttgtctcta aatatactac ggggccaaga
     6781 atagtagtgt attcattgag aagttcacat ggcacagtat aaaaatctaa atcgataact
     6841 tgttcaataa ctgatttatg aatatctatt ggaataagtt ctgcatcatt aaattcaaat
     6901 gtatcatcaa gatcctcttc cgtacatatt ccttgaataa aattgagatt tccgataccc
     6961 atgggaaaaa gttcatcagt cgaaagttct agagacggtt ccccatcata gttaagagta
     7021 aggctataat ctgagataac aattttgctg gaatattgtc gtgatctaaa aggaatttga
     7081 tcaatgtatc cgatagggat ctcggcatat tcggaaagac cacatggctt atctggacca
     7141 tccatagcat cttttaaaat gttatcaatg gtatattctt ccccggcggt agcccattct
     7201 cgtccttttt taatatcatt aggccaatta ttcacgttat agatcacatg accaaatctc
     7261 tgaacttgtt cccagacgta ttcgtaatca agattcatcc ctggattttg aggttcttcg
     7321 cttgcttcaa cagggctttt tttcagaaaa caaaattgta gtcattgcac tggatatcat
     7381 cagcattaca acaaaaattc caataatttt cttcttcatt tttttacctc caattcaagc
     7441 atgtttcttc ttgtttatat aatacagttg ggggtcgatg aaagtattca ctggctttgt
     7501 ttcaaaagta tttaatctct cttgaaacaa tttaataaaa cgaaagagta gatcaggatg
     7561 tacaaccgag tttcgagaca atgccgtatt cacttttttt atgaaaagaa taataaagaa
     7621 gttcttggat attattcttg gggaatgtaa gtaatgaaat cagcttggtg gaaacttttt
     7681 ataattctga ttgtcgttat catggtgtgc ccagttgcaa attcattagt atttggtgaa
     7741 atcaataaaa attcaattgt tcaacagagt ttaattattc cagagtatat atcagattgg
     7801 tatattgaca aaagcagtat tcgacaggac catgaaccat tttctcttga tttaagacag
     7861 aatcagtcga ttgtcaaaaa agcacagtat catacccaag gagatggcga taaaaaaggt
     7921 ccgatggatt ctccatggcc gatgttcgga catgatgcag ttcacacctg tcgcagtccg
     7981 tatagcacgg aaaataatac tggtgtggag atttggcgtg ttcgtagtga taagatgggt
     8041 tctgtttggg gttctgcatt aacagataac aatggattga tttattttgg aacaaaggca
     8101 tctgatgatt cattgtatgc attgtatccg aatggaacac gaaagtggaa attccctgcc
     8161 actgctttga tttgggggac ccccgcaatc tcagaagaag gaacaatata ttttacaaca
     8221 tggggacgat atttttttgc agtgaatcct gatggtacgg aacgatggcg tttcaataca
     8281 ggtagcatat cagtatcttc tgttacaatt gctgaagacg gtgcacttta ttttggcgat
     8341 gataataata tcatttgggc attaaatcct gatggcacgg agaaatggcg atataccact
     8401 ggctatatcg tgatgggttc tcctgcaata agtatccaag atggtacaat ctacattggt
     8461 tccggtgact attacctcta tgcattgtat ccgaatggaa cgctccgctg gcggttcgcg
     8521 acaggagact atataaaagg ttctgcatca attgcacctg atggtacgat ttatattcct
     8581 tcgtttgatg aatatctcta tgcattatat ccgaatggaa caaagaaatg gcaagtttcg
     8641 acaggtgata gtctggcagc tgctggtgtt gccattggta aacttggtac gatctatgtc
     8701 gggacggaaa tgttacgagc gtataatcct gatgggacgt tgaaatggat tacagacgtg
     8761 caaggttaca tggaagggac ggtccctgca gtttccgctg atggcacgat ttacgtcagc
     8821 gcaggtaact cgcttgttgc ggtgaatccc gatgggaccg agaaatggcg tatcgcctta
     8881 tctgatgagc aaattcgttc atctcccagt atcggtcctg atgaccgtgt gtatgttggt
     8941 tctacataca gtgattatgg gtatcttcat gcattaggcc ttggcccact atatgccgag
     9001 gcgtacggtc cttatagtgg agttgtatct gagtcgatac agttcattgg tgatgctttc
     9061 ggtggtaccc caccttatca atatcattgg gatttcggag atggtaccac atcggaagaa
     9121 caaaatccag ctcatagctt tgcagatatc agaacgtttt tagcgatgtt ttctgtgact
     9181 gattcggagg gaaacaccag cgaagatacc gcgcaagtca caattactgc gccagcacca
     9241 accgtcacca tcgtcaaacc agtgaatggt atctacatcg gagactcaag aatccttccg
     9301 tttttcagac cattcattat cggtcaaatc accatccaag tggatgcgac tcaggagccg
     9361 tatggcattg atcatgtcga attctatata gatggtgaga tacgagcgac tgtcactgaa
     9421 atcccttatc aatggacatg gaatacacga acattcttca agcataccat caacgtgatt
     9481 gcgttcgata ccattggaaa aagtacagaa aaatccattg acgtatccaa gttcttttag
     9541 tagctctgcc ctcttaaata aatataaaaa gaaatggaaa atcaggcggt aatttcctga
     9601 tttccactgt tttggatcac tgatgattac ttcgttacgt tttgttgaac tgccaccaag
     9661 cctcaaggtt cacatcatca tttgttctta ggatgttccc ttcaggatct ctgaatgcag
     9721 cataaacacg ataggtgcct gtgccatggg tcaaatccga tgctctcact agtccattga
     9781 agatggtgtc gagtgcgagt tgatcaccgc tggtgatggt tcgtggtatg gtttcattga
     9841 tagtatcatt gtcgacgatc catctcgggg cagtacctct cccggtatcg tagtattgca
     9901 cttgtatcaa caagtatcct ctgatatctg tggatccggt gtttttaatc ttcgactggt
     9961 tccggtacgt gatgcaaagc ttgggcatat actgcggggc tccattccgt gaatacacga
    10021 tgacgtattc cgatgtagtt ggtgccgtac cactgatatc cttactgctt agctctgttc
    10081 ggaatcataa aagaagatca tctcataaca tctaaatact ttggcaccgt gctttataca
    10141 gtcgacccag gatgatgata ctattccctt ctctatactt ttcaatacaa atgattagtg
    10201 ttcaacagaa ttctacatca tatttttcgt actaaaccac gttttcatcc cagtatcaac
    10261 accttttaat ccatcaggta aatcttggta gatctttcaa tgcacattcc tgtcagattt
    10321 tacacatcct cctccttact ttcggaacta ctgagtgaac ctttactttt atatacgagt
    10381 agtttattag ggagttccct taagaatcga tagcggagtg taaagaatgg cacgaatcca
    10441 cgcgagaaca aaaggtaaat caggttccac tcatcccatg cgggaaaaac acccagaatg
    10501 gagctccctt aaccctcgag aaatcgaagg gcgcatcctc gagcttgcaa aagctgagaa
    10561 gtccaccagt gagatcggga tgatcttacg tgatcaatat gcggtccctg atgtgaaaat
    10621 cgcgacggga aaaaccatta cgcagatcct tgaacagaac aagattaccc aggagatccc
    10681 tgaggatttg cagaatctca tcagaaccgc gttgaaactg aagaaacaca ttgatcaaca
    10741 taagaaggat tttaaaacaa aacggagcct gtcactgaca gagtcgaaga tccgccgctt
    10801 gatgaaatat tatcgtcggg aacagcgact tccagtggga tggaagtaca gtttagacca
    10861 agcaaagctt atgttcgaat aagaaatctt ccagtatctt tttagcctgg aacatgtttt
    10921 tcgttctgta gagtacaatc tatggcagaa gagatatccc aagcctgtca ggatgcagca
    10981 tccatgctgc agtccattcc atcttcgcga cgaattcgag tggtgtccca ttacgatgca
    11041 gatgggatcg ctgcggcagg ggtgctctgc accgcggtgt accgcgcagg gtacgagttc
    11101 catgcgtcat tgatgcgcaa ccccttcaca aaaggctttg accgcttgaa aaacgagcat
    11161 aacgatctga ttattttttc agatatgggc agtggccaga tagagactat cgaatctctc
    11221 ggctgcaaag taatcgtcct tgatcatcac cagtatctca caacccagac gtcgaagaac
    11281 atcatacaga tcaacgcgaa cctctttggc gctgatggga attatgaggt ctccggtgca
    11341 accttaagtt atctgtttgc aaccgcactt gacccgtcaa acgaggatct cgcaccactt
    11401 gcgttggctg gggcgactgg tgataaacag ttcatcggcg gcatccgtgg gttgaacagg
    11461 accatcctgg acaatgcgct gcagaaagga ttcctcactg aacaaaccag catcaaactg
    11521 tacggagaaa cgatcgcgga tgcgttgtat tattctgtgg acccctacta tccgggtctc
    11581 tccgggaagc atgaaaaaat cgaaacgatg ttggaacaat tgcacataaa gaaaaccgct
    11641 tccatccagg aaatcccctt agaaaccatg gcgaagatac agtcctatct tctcttcgca
    11701 ttaatcaaag caggttgtca acagaacatc gtagatatga cgatacgaaa acggtacatc
    11761 gcatcacaag gctgggaatt ggagcgtttc gctgatctgc ttgatgcgtg cgggaaaaac
    11821 gggtaccgca gcctgggtct ttctctttgt cttggtgatg gcaccacgtg gaacgaggcg
    11881 gtgcaagtcg agaaagacta taaacaaaaa ctcctcgacg ggcttcaagg cttagaacac
    11941 ggtggtgtcc aagagaccga tgggatgcgg tatttcttct cggataattc ttcgcttggt
    12001 ggggttatcg ctgggatcgc gatgaactat gtgctcgatg agaagaaacc actgttctcc
    12061 ctgacgagaa aagaaaccga tgatgaagta catatctcat gccgaggaaa tcaacaactg
    12121 gtgcggcaag gtcttgacct gggtgttgcg atgaaaaccg tggcggggga tcttggtggg
    12181 tatggcggtg gtcataagat cgctgctggt gcaacgattt cgtttggaaa agaaaaagaa
    12241 ttcttagaac aggttgatag gttacttgtt cagcagatgc agaggtaata tgaaggcgtc
    12301 gtgtacgatt atcttggaat ttgagagtgc agagaaagca aaaaaggtgc tccggtccat
    12361 tcaggttgat gaccaggggt ttgtacgatc aaaggtcacg gataagacgc ttgaggcggt
    12421 cgtggaaagt tcatctgttg cctcgttgct acatacgctt gatgattatc tcgcctgtgt
    12481 ttctgtcgct gctgacattg taaagaaata acagtatgat ttcgacctgt ttcatttgtt
    12541 ttgatggaaa acgtaactat cctccctttt gtcatctcca atgaaaaata aatattggta
    12601 acaatattta aatacgtccg ttcagttcta aatctgaggg aaaaaagttt tagaggaagg
    12661 aactatgaaa aagaaagtag ttggatgtgt actttttctg ctgctgatcg gaacgaccgt
    12721 accattgata tcatcttcaa atagtgacat tcatcccgtc ctcgccgatg aaatcaccct
    12781 ggcggaggac tgcgagtgtg aatcaaatca ggttctcacg tatcaaataa ccgtgaacga
    12841 taccgtaggt caatcaccaa aaccaacaat ctcacatgat ctcccaccgt atttcagttg
    12901 gaaagacgta gacggtgtcg attggacgac cacagccaaa gatcaagggg actgcgggag
    12961 ctgctgggat ttcgccgcaa tgggtgcact ggagagcatc atccaaatac gggagggatg
    13021 cgcaggattg aatcttgatc tttccgaaca gtacgtcctg tcttgtttgc atgccgcagg
    13081 gagttgcaat ggagggtggg cgtacaatgc gtataaatat attaaaagta acacgtcctc
    13141 agggaactac tgtaatggga tcataccaga attctgcttc ccgtatcagg tcaatgacga
    13201 tgtaccctgt tccaacgcca gcccggattg gagcaacttc ctcatcccgc tctccaccta
    13261 tggacggtgg gtgcctgatg ggagtgtcga ggatcgtaat gcgatcaaga cccaaatcat
    13321 ggagtccgga ccggtcgttg ccaccatgct gttcacctac tacgcccatg gttcagacaa
    13381 tctggaggaa tggggctata cccatcacga ctccacggac tattatgcgt atcctggccc
    13441 gatgcaaggc accaatcatc aggtcgtcct tgtcggatgg aatgacgaca tctccatcca
    13501 gaacggcggg tactggatcg tgaaaaacag catcagtgaa gaatggggat atgatggttt
    13561 tttcaacctg gaatacggca gcctcaacat cgataaaacc gatatcaact gggttgatta
    13621 taatccagag aattatagta actgggtacc ggtcgcacgc atcaatacct ccgccttgga
    13681 acagatggtg tttgacggaa gtgaaagctt cgaccatgaa ggcgccatcg tgtcgtacgc
    13741 atgggatttt ggtgatggat ccaccgcaac agggatgacc accacccata cctatgatgc
    13801 cgcgggaatc tatcttgtca cactcacggt ttcggacaat gccagtaaca ctgatgatga
    13861 gaccgcatgg gtgttcatca acacggaaaa tcatcctccg aagacgccat ggctccttgg
    13921 acggagaact ggggaaaacg acaccgcata taagtacaca ttctgggcga ccgatcctga
    13981 cggggatgac ctgtattatt atctgaactg gggggacacc tactgggatg gtggagcagt
    14041 cggatggata ggaccgtata aatccggtga gaaggtcacc ttggaaaaaa cctgggcgga
    14101 aaaaggcaac tacacggttc gtgtgaaagc atccgatcga tacgatgcaa aaagcaattg
    14161 ggcgaccctg aaagtcacaa tgccactgtc ctatgatctc ccgttctatt ggttcctgga
    14221 tcaattgctc gagcggttcc cgcatgcgtt tccgatcctc cgttatctgc gcagtggtta
    14281 aaccaaaaaa ttttcctttt ttttatttta accgacgttg tcgttgggaa atttccctcg
    14341 cttttttctt atatttgagt caacaaggat aatgagttta gatattccag aatgaataac
    14401 tttcttatat gagaacattg ttataaaatt gtaaaggagg aaaatcattg aatcgaaaag
    14461 gattggctgt tggattaatt cttttattga ttggaacgag tatgccaatt actgcacaaa
    14521 atgcaaagaa agcatctgaa tcaacttcaa gaggtaactg gttgtatgtc ggtgggagtg
    14581 gaccagacct tcggaaagca tattcctata attctttaaa aactacaatg gaaagtcctt
    14641 tactacaaaa tgataattgg actcaaatcc aaaaactaat ttcactcgat gggaaacccc
    14701 atgatgcctt tggttattcc gtatcagtcg atggtaacac tgctctcgtt ggggcttggt
    14761 ttgataatag cactcaagat ttatctggat cggtctatgt ttttattcat tcctccagac
    14821 tttggactct acaacaaaaa atctttgctc cagatattac agattttggg ttgtctgtat
    14881 cagtatctgg agatactgca cttataggcg ctggaggggc agcttatatc tatgctcgta
    14941 acgggactca atggtatctg gtaactaaat taacttcatc taggatatca ttcggaagag
    15001 aagtttctct ttctggagat acggcgatta tcggagagga ctttggcgag aaagcttatg
    15061 tgtttcgctg tgtgaactca acgtggaatc aagaagcgat actcactgct tctgatccag
    15121 agccaaatga ttttttcggc accgatgttt caatcgatgg ggacacagca ctggtgagtt
    15181 cactacagga cgaagataat ggggtaggtt ctggttctgc ttatgtcttt gttcgaaatg
    15241 gaacgatttg gacccaacaa acaaaattac ttccttccga tggtaaacct atggattggt
    15301 ttggctggtc tgtcgatatt aaaggtgata ctgctttcat cggagctcca acttattggt
    15361 acgatgatcc gggttttgtg tatgtattta ctcggaacgg tacattatgg actcaacaac
    15421 aaaaactcct ttcctcagat ggtgtgggtg gtgaccgatt cggatgttcc atatctttaa
    15481 atggaaatct tgccctcatt ggagcatatt ttcatgatca catgggttac gattctggtt
    15541 cagcatacat ctttgcattc gacggtgaac aatggaagga agaagttgag ttgcttgcct
    15601 ctgatggtta tgaaaagaac tggtttggtt tctccgtttc actgttcaat ggaaccgctt
    15661 tcattggagc cccgtatgaa atggtgtctg ggttcaatcc aggattcacc tatgtatttt
    15721 ccgaagatag ttggaatcct caagtaaaaa ttgagattca gggtggttta ggattaacca
    15781 ctatcattac gaatcttgat ctaacagaaa atctgaacga tattcaatgg cattgttcag
    15841 caataggtgg tcttctatgg aaaattaact atgagaaaaa tggtatagta aatcaaatta
    15901 aacctggaga atctataaaa gtccgttcag gtttgatttt tggactgggc cctgttttta
    15961 tctccttcaa tgtagcggat gccgaaaaca caattaaagc aaaacaaatt attttttaca
    16021 cggtatcacc ataaaaacct cggattatga caattaacga aaatcgaacg attggaatcc
    16081 tgccaaagaa ccctatgata ttcccataat gaattgaaaa gagaccgtca gaaattgtcg
    16141 atgatacaaa ggagataatt tcctgaatct ctctgttttt gtaagtaaga attaagtatg
    16201 catgggagga taacctgccg atgatgcgat tcgagttaca agcgagattc acactgagca
    16261 gcgatgtgac aaaactgaca aaagagtttg acaaattcat cgcagagacg aacgaaacga
    16321 tcctcaaaaa aggtccagag aagctagcag tcatcgagaa atataacatt gaaaaaacaa
    16381 cactgtcttt gtccatttca tcggaaggga cccttcgacc acacaatgcg ctgctgcaaa
    16441 tcaaaaacgc actcaccaaa gagctgggga aaacccatca tgtcggcgtg cgtggtatca
    16501 ccatagaaaa gtatacgata tcttttgacc tcccccgtga accactcaag gacatcagca
    16561 tcccgttcgc tgaggtcaca ataaagggga aacaggcgac gatgctgctg tcagatgtct
    16621 ctgaggagtt tctccgcagg aactaca
//
LOCUS       DATMXJ010000140        14378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_13170, whole genome shotgun sequence.
ACCESSION   DATMXJ010000140 DATMXJ010000000
VERSION     DATMXJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14378
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13170"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /locus_tag="VN377_03805"
     CDS             <1..2282
                     /locus_tag="VN377_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWR27546.1"
                     /translation="FILTQYEPYEIEFIYGNRDSNYLHLTFYFYTNDQTSNFRNESIM
                     YQSNIVENTVYYRIISWDNQTSRYALLYADQPGLGTQKHGIISFLDGTTLVGMGGTLD
                     SNTYSDEQLKTMLDMVEEKARELLSIKSSMDELSGHITGFTRPMKHIKVSLSNGGSTT
                     ETTTDAGGYYNITNPLTQGTEYTLTVTFSYVIDTTTYFSLHYHENNATVVSLQRTFTM
                     DSATDLTQDIALESELPRYYGGDWAKTFASMYAHFTEALELYKDLLRVNVDFQLPVDV
                     YAFASEQTGTRYWYNIPGKSYITIDAEKSIHESVYRPMNREYHEFSHYIMHTLYQRWP
                     APEPSLSIGEWNHDGYLNPSTSDSYVEGFAEFMSAVIMEYYEQAGTSIAMIPPNEKDK
                     SAFAAMDYMFNTQWNGKEIDCPAWADNGRSEETAVAGVLWDLYDDNSDYCDKTPAEMY
                     QLYQQMLPQLQKDYEKYKADFEQDTIEYGGNATPRPFVQATLQDFEDYKYDDDNATLG
                     LEAVWAVIKNFHNDFTSVYNDFISKYPAQKKVIDEVFIRHAFYVDTDPGNGEYDLHDI
                     YRDENHNRKYDAGEYYVDCPVDGFHYTTEEVIGQATNYNRLWRQSMQEIPGYFIKVDN
                     TVPFYLVKVSFPNAFYLDYVVRVWNENGMINIPVPPEGYASLVTVIPEGVQRSVPLSF
                     TSDVFQRHYDSSLAQGYYVQHDFDITGPIPPLPSIPSDTGDTDNVPGQKTQTPGFEMI
                     VFLAACALVCLLLKKTKRT"
     gene            complement(2509..5205)
                     /locus_tag="VN377_03810"
     CDS             complement(2509..5205)
                     /locus_tag="VN377_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27547.1"
                     /translation="MRNNKSKKKLVIQQLTSIILFLFFITVSFTTTINASQKQTGGEL
                     SINVEYLTYSMAFSNPSFTETRLNDYTFASVEMQGCLSLGRNPGDPAVPVKFIQLLIP
                     AMKTVAGITVEGDAVDGTPFRIDLRETPIVPSQPPIPIGSPPSGIIKNDQIYQSSNPY
                     PTTIYDNQYQVGYCRGFTILSFAVTPIQYTPSEGTFRYYPILTFTINLNDEEQPNEFY
                     KGTTEDEAWVKTLVSNPEITETYKGLPPSPLEYPGGLCDPSHQYDYVIVTTTQNGLDF
                     WQTGGSTPYNWDSLMNHHLADGLSSTLVTVQDINACPGYQNSDPLFNDSQAHIREFCK
                     DAYEDWGTRYILFAGDSDKVPVRLLNYAYDYEETNNGVESDLYYSNLDNNFNANHNTR
                     WGEEDDSGFDLYSELYVGRLPCDAPQDVSNWLTKSFYYADSTDLKYLNNTGFYAGDTT
                     WNCQGDDFIDYSAIKGTNDWLGPNPHSDGPFPSWAGFQYGFETWNAVNPGVWYNLSVK
                     WTAEPPNPGWQGGSTSAATAGLRTAINNDEVTLLSAIAHANEHMSCDVYDYQWASQYH
                     NTKPFFITDYGCHCGDFDVDDDSMVDVMLYDSATDLAFGCVYNTGFGWGNSVGTNASS
                     AFQQKEFWDYFFDLTNNSGDFSNWQLGKGHAWSKDRMAPTLNWDYSYGTWRGVIECCL
                     LFGDPAQKIRPPRTNTAPYKPSPPNGPTEGEAGIEYRFTATTTDPENDSLLYQFDWGD
                     ETMSDWGTLYDSGAIANATHLWILGGTYQICVRVKDVVGAMTEWSDPMSIYIGQPVIQ
                     IAKIKGGIGVRFNLVNNGDGNATNINWNATIVGETSGITMQQQGSIPSLGVGQTTSIK
                     PFGLFIGFGKLSITVEAVPFYGNATKQKVDAFLLGIFVLILK"
     gene            complement(5242..6459)
                     /locus_tag="VN377_03815"
     CDS             complement(5242..6459)
                     /locus_tag="VN377_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27548.1"
                     /translation="MKKIVSILIGALMCVTVIYSVAGTPTLNADADHVRSNLSQPAWS
                     ENVSWDNGLPDGRNGVSIGGWPGYTREVIDDIHLSDPQFITGGSFRIVTYSGSGPGQI
                     TGVTINIYPDSGGAPGTTVYATETASFTAALTGSMYFSRPEILYTCSFDAIVLPPGTW
                     WVGFYPTSVENLYWLTSTKVGTSEIYFAYPDEGYNKWTAGHLNFNEYYDVSFTLSGEH
                     AVDTTPPVTMCVLIGDMNGSIYANDVTVVLSATDFFTGINYTKYRLDDGGWNTYTTSF
                     IVTANGEHTLYFYSVDLVGNFEEAKNTTFTIEHDVSVEITLKGGLGANAVIKNVGTKN
                     LTGLPWDITLSGGIIFLGKQISGSVDIAPGAEITVKDFVFGFGKTIITATAGNVEKTT
                     TGKVILFFISGVA"
     gene            complement(6500..7735)
                     /locus_tag="VN377_03820"
     CDS             complement(6500..7735)
                     /locus_tag="VN377_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27549.1"
                     /translation="MKERHHNKILNAAGALLIVIIMVLSATTAIAVNTNSFGNMNNVG
                     PVKPEKLEYKAGSLFLQPPNLPDDTFWAFMTSSVQGGYIGYEKYWDVNQPIGSIHWWG
                     TTLYIAGGVWNVGSPVGMRFNITFCNEDWAGRPGSPVVNYSDVTTTYVDTGITYDGVP
                     CYYWSASLNPPVVNLSSAWISIQSIYSPTSPPSWFLWLSSSVGDGISFQGINPNQWDL
                     SLNLTLPDTNPPVTTCHLAGTMNGSIYVSNVTVGLTATDADSGVDYTLFKLNDGDWTT
                     YTAPFIISEDGTYNLSFKSVDFAGNWETEKNISFTVQHPPAFEITISSGKGLNVSINN
                     SGWKTMQFIPWSINITGGIIFIGKSKTGQILQLKPGENWIIKFPVFGFGRTTITVAVG
                     DVEQTAKRFVFLSYVLLTI"
     gene            complement(7949..11788)
                     /locus_tag="VN377_03825"
     CDS             complement(7949..11788)
                     /locus_tag="VN377_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR27550.1"
                     /translation="MKKIKIYKAVIAIAITMAFVMPVSAFANGETNKITSNGNNNIAI
                     NSNNRIGTISSDTPREDTIVAEKTDSTTNLRTRGTIYVDNDQSPGWYDQTHVKTIHEG
                     VTNATAGDTVYVYNGIYYDHVIVNKKLSLIGESRDGVLVDGSGTGNVFYITTPTTQVN
                     ISNFSITNGQYGIYNFKSSFNNISNCNVYRCTSHGIYIYTTSNRNSVMNCNLYNNTGN
                     GISITTSSNNNKIMNCNVYNNNLGILFSRSPMTAIPVANNSITNCNVYQNRATGISLA
                     STINATLSGNTIYNNLYNFGVSATGTTYIGLGMFDHHIDPSNTIDGKPIYYLNKVKNT
                     TIDQTYNAGFIGLVSCYNVTVKNINLRAAGVIVANTTHSTLSNITSSYSGGNGIFFYS
                     ASNNTITQCTLYNNSNGIFFIQASNNNLISNCRVYNTTTYGIGFNWLGSGPGCSYNII
                     TNCVLYKNMYGLRLAGTIHNNSVTNCHAYNNSIGFVPGPYSIIANCEADHNGIGITGS
                     SYGTIINCTSHDNTQEGIDDTALYSGQNVSIINCTVYNNPIGIGKYSAPTGIITHCTI
                     YNATYGIYMQNSADNNNINNCIIHNTSYGIYIRNCVYNHINYNNLLGNGYGVWIQESS
                     SFNIIDHNNFVNTVANGYDSTTDIWDNGSGGNYWSDYTGIDADLNGIGDTPYNISGGS
                     NQDRYPLMLPLDLTPPMITNVQATPVAQRPNEYVNITCTVTDNWNMLDTVKLNVSGPG
                     GFTLEIPMNNGGTYFYNQTYASLGVYNYFIQADDVQGNVGTSDIYSFVITDLDKPTSA
                     VNPLPVWKTATPFAITATAYDNTAVSNVTLWYRYSSDGTSWTTWTSYGTDIAAPWSWS
                     FTGIDGHYQFYSVAVDNYGNIEDAPSTADTSTGIDTTKPVTTATLTPLTPDGQQEWYV
                     STVLVTLTATDALSGVQTTWYRIDAGGWTLYSVPFSTSDGIHVVEYYSYDLAGNIEIT
                     NSVSFKVDTVAPSTTLALQGLIGQQGWYVTNVTVTLTAYDATSGVNYTKYKLNDGSWT
                     VYSGPFTLTTNGNYTLSYYSVDFAGKTETTQQTSVQIQHDVLPPVTTPEFNGVMGNNN
                     WFTSPVTVTFNAVDDSAGVASTKYQLDAGAWITYTGAFTVTDDAEHTILYYSIDNVGN
                     TETAKGAILKIDQTVPTINITVEKTGLIRWLSTANVNDDTSGIARVDFYLDGVILGNV
                     TEAPYEWEYTSQGLAQAIVYDNAGNNAISDAIPVSVDLNLNSKTTTNDQVVVGSQSQS
                     IGSTSTSFQSLFRLSRL"
     gene            12063..12245
                     /locus_tag="VN377_03830"
     CDS             12063..12245
                     /locus_tag="VN377_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27551.1"
                     /translation="MWDYQSGDFFYDSPAIADGMVYVDSFDHKMYCLLTVTAEVPESQ
                     TARSQNGTILLFFIRL"
     gene            12724..>14378
                     /locus_tag="VN377_03835"
     CDS             12724..>14378
                     /locus_tag="VN377_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27552.1"
                     /translation="MMKNRIENKIFVFGLMIFVIGAVYLPSIGAEIGRNTPTMSDSGY
                     RSASTTDWWMMFRHDASHSGYSSSSAPQTNNVQWTYSVGLEMSGSPAVADGKVYIGSW
                     DKKFYCLDATTGVKLWEYLTGLEITSTPAIMNGKVYIGSNDGKVYCFDANTGVKLWEY
                     LTGADVVSSPTIVDGNVYVGSRDHNVYCLNAETGANVWNYTTGDDIISSPAVSDGKVF
                     IGSWDDKVYCLDATTGAWIWEYTTGNDIYSSPAVVDNKVYIGSSDNKLYCLDALSGSH
                     LWDYTTGASIESCPAVTNGKVYVGSFDNKAYCLDASTGEKIWEYLSGDFFYASPAVAD
                     GKVYLGSFDHKMYCLNADTGEKIWSYTTGYIVWSSPAVADEKVYIGSGDHKVYCFKDA
                     NAAPEIPAVPTGPSIGQKGVNYTFSTVTTDPEGDQVFYLFDWGDGTNSNWVGPYNSSV
                     PGTTSHAWADAGNFSIKAKAKDTNGAESDWSATHSISITLGSILEIQDISGGLFKITA
                     VIKNIGDTAATNISWSIKVVGGAWIGKDTTGDINTLAAGGQQK"
BASE COUNT         3828 a         3229 c         3200 g         4121 t
ORIGIN      
        1 ccttcatcct cacccagtac gaaccctacg aaatcgagtt catctacggg aaccgagata
       61 gcaactacct gcacctcacc ttctacttct acaccaacga tcagacatca aactttagaa
      121 acgaaagcat catgtatcag agtaacatcg tggagaacac agtctactac cggatcatct
      181 catgggacaa tcagacaagc cgctacgcgc tactctacgc ggaccaaccc gggttaggca
      241 cccaaaaaca cggcattatc agctttctgg acgggaccac ccttgtcggg atgggcggga
      301 cccttgatag caatacgtac tctgatgagc aactgaagac tatgcttgat atggtcgagg
      361 aaaaagcacg ggagctcctc tcaataaaaa gcagcatgga tgaactcagc gggcacatca
      421 ccgggtttac aagaccaatg aaacacatca aagtctccct ttcaaatggc gggtccacca
      481 ccgagacgac cacagacgcg ggcggatatt acaacatcac caaccccctt actcaaggaa
      541 cggagtacac ccttaccgtc accttttcct acgtcatcga caccacaacg tatttttccc
      601 ttcactacca cgagaacaac gcaaccgtcg tgagcttaca gcgcaccttc accatggact
      661 ccgctaccga ccttacccag gatatcgccc ttgaatccga actgccaagg tactacggcg
      721 gagactgggc gaaaacattc gccagcatgt acgcgcattt cacagaagca cttgaattat
      781 acaaggattt attgcgtgtg aatgtggact tccagctccc ggtcgacgtc tacgcatttg
      841 catcagaaca aaccgggacg cgttactggt acaatatccc tggtaaaagc tacatcacca
      901 tcgatgcgga gaaatcaatc catgagagcg tgtaccgacc catgaaccgg gagtaccatg
      961 agttctccca ttacatcatg catacgttat atcaacgatg gcctgcgcca gagccgagtc
     1021 tctcaatcgg agagtggaac catgatgggt atctcaaccc cagcacctcc gactcgtacg
     1081 tcgaaggatt cgcagaattc atgtccgcgg taatcatgga gtactatgaa caagccggga
     1141 catcgattgc gatgattcca ccaaatgaaa aagacaaaag tgcgtttgct gctatggact
     1201 atatgttcaa cacacaatgg aatggcaaag aaatcgattg tccagcgtgg gctgataacg
     1261 gcagatctga ggaaaccgcg gttgctgggg tactctggga cctctatgat gataattctg
     1321 attattgtga taaaacccct gcggaaatgt accagctcta tcaacaaatg ttaccacaac
     1381 ttcaaaaaga ttatgagaaa tataaagccg actttgaaca agatactatc gaatatggag
     1441 ggaacgccac acccagacca tttgttcagg cgaccctcca agactttgag gactataaat
     1501 atgatgacga caacgctacc ctcggacttg aagctgtctg ggctgtcatt aaaaatttcc
     1561 ataatgattt tacctcggtc tataacgatt ttatttccaa atatcccgca caaaaaaagg
     1621 taatcgatga agtgttcatc agacatgcgt tctatgtaga taccgacccg ggtaatggtg
     1681 aatatgacct tcatgatatc tatcgtgatg agaatcataa tcgaaaatac gatgccggag
     1741 aatattacgt ggactgcccc gttgacgggt ttcattacac cacagaggaa gtcatcggtc
     1801 aagcaaccaa ttacaacagg ctctggagac aaagcatgca agaaatccca gggtatttca
     1861 tcaaggtgga caacaccgta ccattttacc ttgttaaagt ctcatttccc aatgcctttt
     1921 accttgatta cgttgtccga gtgtggaacg aaaatgggat gatcaatatc ccggttccac
     1981 cagaaggata cgcatcatta gtcactgtga tccctgaagg cgtccagagg agtgtaccat
     2041 tgagttttac ctcagatgtc ttccaacgtc attatgactc atcacttgca caaggatact
     2101 atgtacaaca tgatttcgac atcaccggac cgatcccccc actaccatcg ataccatccg
     2161 atacaggcga cacagacaat gtcccagggc agaaaaccca aactccaggg ttcgaaatga
     2221 tagtgtttct tgcagcatgt gcactggtct gtcttttatt aaagaaaaca aagagaacat
     2281 agacgaacaa agagttcaga gaaatcgtat gacttctaaa catcattcat tatgattatc
     2341 tgattagaac ccaggtggaa tattcataca ttcgataaga ttcaaatcgc cattcaacaa
     2401 cacaccaaca tctgcaaatt gattactgat ggaatactgc cggtatcttc taccttctcc
     2461 gttttcgata tcaggtgttc ccgtatttca ttaggatgtt ctcatctctt atttcaatat
     2521 aaggacgaag atgccaagga ggaatgcatc gactttttgt tttgtcgcgt tgccatagaa
     2581 cggcactgct tctacggtaa tcgaaagttt cccaaatcca atgaacaatc caaagggttt
     2641 gattgatgtg gtttggccga ccccgaggga tgggatgctt ccctgctgtt gcatggtgat
     2701 accgcttgtt tctccgacaa tcgtcgcatt ccagttgata ttggtggcgt tcccatcacc
     2761 gttattcacg agattaaacc gtacaccgat tccaccttta atcttcgcta tttggataac
     2821 gggttgacca atgtaaatgg acataggatc tgaccattcg gtcatcgcac cgaccacatc
     2881 ttttacccgt acgcagattt gataggttcc cccgagtatc cagagatggg ttgcatttgc
     2941 tatcgctcct gagtcataga gtgttcccca gtcgctcatg gtttcatcgc cccagtcaaa
     3001 ttgatataaa agggagtcgt tttcaggatc ggtggtggta gcagtaaaac gatattctat
     3061 ccccgcttct ccttctgtcg ggccattagg tgggcttggt ttgtaagggg cggtatttgt
     3121 gcgtgggggt ctaatttttt gcgcaggatc gccgaacagc agacagcatt ctataacccc
     3181 tcgccaggtt ccataggaat aatcccagtt gagagtcgga gccatccggt cctttgacca
     3241 agcatgacct ttcccaagct gccaattact gaaatctcca gagttattcg tcagatcaaa
     3301 gaaataatcc cagaattctt tttgttgaaa cgcactactt gcgttcgtac caactgaatt
     3361 cccccaccca aaccccgtgt tgtaaacaca gccgaaggcg agatcggtag cagagtcata
     3421 cagcatgaca tcaaccatgc tatcatcatc aacatcgaaa tcaccacaat gacatccata
     3481 atccgtaatg aaaaagggtt ttgtattgtg gtattgactt gcccattgat agtcgtagac
     3541 atcacaggac atatgttcat ttgcgtgcgc tatcgcactc agtagggtaa cttcgtcatt
     3601 attaatcgcg gttcgtaacc ctgctgtcgc tgcagacgtg cttcccccct gccagccagg
     3661 gttgggtggt tccgctgtcc atttcacgga aagattgtac cagacgccag ggttcaccgc
     3721 attccaggtt tcaaacccgt attggaaccc agcccagctg gggaacggac catcgctatg
     3781 aggattcggc ccaagccagt cattggttcc tttgatcgcg ctgtaatcaa tgaaatcatc
     3841 tccttgacaa ttccaggtgg tatccccggc ataaaaccct gtattattga ggtatttcaa
     3901 atcggtgctg tctgcgtagt agaagctttt tgtcagccag tttgagacat cctgtggtgc
     3961 atcacaaggg aggcgaccaa catacaattc tgagtataga tcaaagccgc tgtcatcttc
     4021 ctcaccccat cttgtattat gattggcatt gaaattatta tcgagattgc tgtagtaaag
     4081 gtcagattca acaccgttat tggtctcttc atagtcgtac gcataattta gtaaacgaac
     4141 aggaacttta tcgctgtcac cagcgaaaag aatgtatcgc gttccccaat cctcatacgc
     4201 gtccttacaa aattcacgga tatgcgcttg tgaatcattg aacaatgggt ccgagttttg
     4261 gtaaccggga catgcgttga tatcttgaac agtcacaagg gtagaactca gaccatcggc
     4321 caaatgatga ttcattaaac tatcccagtt atagggagtt gaaccaccgg tttgccaaaa
     4381 atcaagacca ttttgtgttg tggtgacaat cacgtaatcg tactgatgac tcgggtcgca
     4441 gaggccacca gggtactcta atggtgaagg aggcagccct ttgtatgtct cggtaatctc
     4501 cgggttggag accagggttt tcacccatgc ctcatcctct gttgtaccct tgtagaattc
     4561 atttggttgt tcctcatcgt tcaggttaat agtgaaggta agaatcgggt agtagcggaa
     4621 cgttccttca gagggggtat attggattgg ggtcactgcg aatgaaagaa tcgtaaatcc
     4681 gcgacaatac ccaacttgat actgattatc gtagatggtt gttgggtacg ggtttgagga
     4741 ttggtagatc tgatcatttt ttataatccc tgagggtgga ctgccaatgg gaattggagg
     4801 ctgcgaggga acgatcggtg tttccctaag gtcgatacgg aatggagtac catctacagc
     4861 gtctccttca acagtgattc ctgccactgt tttcattgcg gggattaaga gttgaataaa
     4921 ttttacgggt actgcagggt ctccggggtt tctccctagc gagagacatc cttgcatttc
     4981 aacactcgca aaggtgtaat cattgaggcg tgtttctgtg aaactcgggt tactgaatgc
     5041 cattgaatag gttaggtatt ccacatttat cgatagttct cctccggttt gtttttgtga
     5101 tgcgttgatg gtcgtggtga acgagacagt tataaaaaag aggaacagga tgatacttgt
     5161 gagttgttgg attaccagct tctttttact tttattgttt ctcatatttc tcccttattt
     5221 atgttatgaa gagaaaggag tttatgcaac tccgctgatg aagaacagga tgacttttcc
     5281 tgtcgtagtt ttttcaacgt ttcccgcggt cgcagtgatt attgttttcc cgaatccaaa
     5341 cacaaaatct ttcactgtaa tctctgctcc tggagcaatg tccacgctgc cagatatctg
     5401 ttttcctaag aaaatgatac cgccgctcag cgtaatatcc cagggaagcc ccgtaagatt
     5461 tttcgtacca acattcttaa tcaccgcatt ggcaccaaga ccacccttca gcgtgatttc
     5521 aacagacaca tcgtgctcta tagtaaatgt cgtatttttt gcctcttcga aatttcccac
     5581 taaatcaaca gagtaaaaat acaaggtatg ctctccattt gctgtgacaa taaaagatgt
     5641 cgtatacgta ttccatcccc catcatccag tctatatttc gtgtagttga tgcctgtgaa
     5701 gaaatcggtt gcgcttaata caacagtcac atcgttcgca tagatactac cgttcatatc
     5761 cccgatgaga acacacatgg tcaccggcgg tgtggtatcc actgcatgtt caccgctgag
     5821 agtaaatgat acatcataat attcattaaa attgaggtga cccgcggtcc atttgttata
     5881 accctcgtca ggatacgcga aataaatttc acttgtccca acctttgtcg atgtaagcca
     5941 gtagagattt tccacacttg tcggatagaa cccgacccac catgttcctg gtggtaacac
     6001 gatagcgtca aacgaacacg tgtacagaat ttccggtctg gaaaaataca tagaacccgt
     6061 gagcgccgca gtaaaggatg ctgtttccgt cgcgtataca gttgtacctg gtgcgccacc
     6121 agaatcagga taaatgttta tggtgactcc tgttatctga ccgggacccg aaccagaata
     6181 ggtaactatc ctgaatgacc cgcccgtgat aaactgagga tctgagagat ggatatcatc
     6241 aatgacctct ctggtatatc ctggccagcc cccaattgag acaccatttc ggccatccgg
     6301 taatccatta tcccaactga cattctccga ccaagcaggt tgacttaggt tagatctgac
     6361 atggtcagca tctgcgttca gagttggtgt gcctgccacg gaatagataa cggtaacgca
     6421 catcaacgca cctatcaata tactcacaat ttttttcatc ttttaacctc catatcatcc
     6481 tctttatttt acattacttt tagatggtga gaaggacgta tgacaggaaa acaaatcttt
     6541 ttgctgtctg ttcaacgtca cctacggcca ccgtaatcgt tgttcttccg aacccgaaga
     6601 ccgggaattt aatgatccaa ttttcaccgg gtttgagttg aaggatctgc ccagtttttg
     6661 attttccaat aaatatgata ccaccagtga tgttaatgct ccatggaata aactgcatgg
     6721 ttttccaacc agaattatta attgacacat ttagaccttt gccgctagat attgttattt
     6781 caaatgctgg tggatgttgg acggtaaacg aaatgttttt ttctgtctcc cagttacctg
     6841 cgaaatcaac tgatttaaaa gataagttat aggtaccatc ttcagagatg ataaatggcg
     6901 cggtgtacgt ggtccagtca ccgtcgttca gcttgaacaa tgtataatcc acaccagaat
     6961 cagcatcggt cgcggtaagg cccacggtca cattactcac ataaatacta ccgttcatcg
     7021 ttccagctaa atgacaagtc gtcacgggtg gattggtatc cgggagggtc agattcagtg
     7081 ataaatccca ttggttagga ttaatgcctt gaaatgatat gccatcacca acggatgaag
     7141 atagccacaa aaaccagctt ggcggtgaag tcggactgta gatgctttga attgagatcc
     7201 atgcactact cagattaaca acaggtggat tgagggacgc actccagtaa taacagggaa
     7261 ctccatcata cgtgatccct gtatctacat atgtggtggt gacatccgaa tagttcacaa
     7321 ctggtgaacc tggtcgaccc gcccaatcct cgttacagaa ggtaatatta aatctcatgc
     7381 caactgggct accaacgttc cagacaccgc cagcaatata taatgttgta ccccaccaat
     7441 ggatactacc aataggttga ttcacatccc agtacttttc gtacccaatg tacccacctt
     7501 ggaccgaaga ggtcataaac gcccaaaaag tatcatccgg cagattaggc ggttgaagga
     7561 acaatgatcc cgctttgtat tctaattttt ctggttttac aggacccaca ttgttcatat
     7621 ttccaaagct atttgtgttc actgcaattg ctgtggttgc agataaaacc attataatca
     7681 caatcagcaa cgcacctgct gcgttcaaaa ttttattatg atgtctctct ttcattttat
     7741 cctacctacc ttttttttat tagtcttact atttatttgg agggattccc gccctactcg
     7801 tttatcgtgt gatgaatagt gatatcatcg tatttctacc atggataggg accatacttt
     7861 tctccccatt ttgataaaat gggggtgggg tatcacatcc ccaccttggg cttagggttt
     7921 tgtttactag ttctcttgcg gccttccatt acaggcgaga tagccgaaag agcgattgga
     7981 atgatgtgga cgttgatccg atgctctgac tttggctacc tactactacc tggtcatttg
     8041 ttgttgtctt gctgttcaga ttgagatcta cactgaccgg tatcgcatcg cttatcgcat
     8101 tattccctgc gttatcatac acgattgcct gtgctaaacc ctgcgaggta tattcccatt
     8161 catatggtgc ttctgtcaca ttcccgagga tcacgccatc tagatagaaa tctactctgg
     8221 cgattccgct cgtatcgtcg ttgacatttg ctgtggataa ccaacgtatc aatcctgttt
     8281 tttccaccgt gatgttgatg gttgggactg tctgatcgat cttgagaatt gctcctttcg
     8341 cggtttccgt gtttcccaca ttatcaatgg aatagtacaa gatggtatgt tcagcatcgt
     8401 cggttaccgt aaacgctccg gtgtacgtaa tccaagcccc ggcatcgagt tgatattttg
     8461 tcgaggcgac cccggcggaa tcatccactg cgttaaacgt cacggtgacc gggctggtaa
     8521 accagttgtt gttccccatg acaccattaa actcaggagt cgtcactggt ggtaacacgt
     8581 cgtgctgtat ctggactgat gtctgttgtg ttgtttcggt ttttcctgca aaatcgaccg
     8641 agtagtaaga tagggtgtag ttcccattgg ttgtaagggt aaagggtccg ctatagacgg
     8701 tccaactgcc atcattaagc ttgtatttgg tgtagttaac ccctgatgtt gcatcatacg
     8761 cggtgagtgt tacagtcacg ttggtcacat accaaccttg ttgaccgatt aagccttgta
     8821 aggctaatgt tgtggaaggt gctacggtgt cgaccttaaa gctcacagag ttggtaattt
     8881 ctatgtttcc cgcaaggtca tacgaataat attctacgac atgaatgcca tcactggtgg
     8941 aaaacggaac agaataaagc gtccatccgc ccgcatcgat cctgtaccat gtcgtctgta
     9001 ctcctgacaa tgcgtcagtt gcggttaagg ttactaggac agtactaaca taccactctt
     9061 gctgcccatc aggcgtcagg ggggtaagtg tcgcggttgt caccggtttt gtggtatcaa
     9121 taccggttga tgtatcagca gtgcttggcg catcctcaat gttcccgtag ttatcaacag
     9181 caactgaata gaactgatag tggccatcga tgccggtgaa cgaccagctc catggcgcag
     9241 cgatatcagt accgtaggat gtccaggtgg tccagcttgt tccatcgctg gaataccgat
     9301 accagagagt gacatttgag acagcggtgt tatcatatgc ggttgcagtg atcgcaaacg
     9361 gtgttgccgt tttccatact gggagcggat ttaccgcaga tgtcggtttg tccaaatctg
     9421 taatgacgaa cgaataaata tcggaagtac cgacattccc ttgaacgtca tctgcctgaa
     9481 tgaaataatt gtaaacaccc aatgatgcgt acgtttgatt atagaagtaa gtaccgccat
     9541 tattcatggg tatttccaag gtaaacccac ctggtccgct cacgtttaat ttcacggtgt
     9601 ccagcatgtt ccagttatcg gtgactgtgc aggtgatgtt cacgtactca ttcggtcttt
     9661 gtgcaacagg agttgcttgg acattggtta tcatcggtgg tgttaaatcc aatggaagca
     9721 tcaatgggta acggtcctga ttacttcccc cggatatatt gtacggggtg tcaccaatac
     9781 cattaaggtc tgcatcgatg ccagtgtaat cactccagta gttaccacca cttccattat
     9841 cccatatgtc agtagtacta tcatatccat tcgcaacagt gttcacaaaa ttattgtgat
     9901 caataatgtt gaaactcgaa ctctcttgga tccagacacc atacccattg ccgagtaaat
     9961 tattgtaatt tatgtggtta tagacgcagt tcctgatgta gatgccatag ctcgtattgt
    10021 ggattataca gttgttgatg ttgttattgt cggctgaatt ctgcatatag ataccatagg
    10081 tagcattata tatggtgcag tgagtgatga tgcctgttgg agcactatat ttgccaatgc
    10141 caatagggtt gttgtatacg gtgcagttta ttattgagac attttggccg gaatataatg
    10201 ctgtgtcgtc gatgccctct tgtgtgttgt cgtgggacgt gcagtttatg atggtgccgt
    10261 aggagctgcc cgtaatgccg atgccattgt gatctgcttc gcagtttgcg atgatggagt
    10321 aaggtcctgg gacaaaacct attgagttat tatatgcatg gcaatttgtc acggagttgt
    10381 tgtgtatagt acctgcaagt cgaaggccgt acatattctt gtataacaca cagttggtga
    10441 tgatattgta cgaacatcct ggtccactgc ccaaccagtt gaacccgatg ccataggtgg
    10501 tggtattgta tactctgcag ttggaaataa ggttgttatt tgatgcttgt atgaaaaata
    10561 tgccgttgct attgttgtac aaggtacact gggtgatggt gttgtttgag gcgctgtaga
    10621 agaaaatacc gtttccgccg ctgtacgaac ttgtgatatt tgagagtgtc gaatgcgtgg
    10681 tatttgccac tatcactcct gccgccctca gatttatgtt ttttactgtg acgttataac
    10741 aggagacgag gccgataaac ccagcgttgt atgtttgatc gattgtcgta ttttttacct
    10801 tattgagata atagatgggt tttccgtcaa tggtgtttga cgggtctatg tggtggtcga
    10861 acatgcctaa gccgatgtac gttgtgccgg tggcactcac accgaagttg tacaaattgt
    10921 tgtagatagt attaccactt aacgtggcgt taatggtact tgcaagggag attcccgtag
    10981 ccctattttg atagacattg cagttcgtga tgctattatt tgcaacgggg atggctgtca
    11041 tggggcttct ggagaaaagg attccaaggt tattgttgta tacattgcag ttcatgattt
    11101 tattgttgtt tgaactcgtg gtaatactga tgccgttacc ggtattgttg tatagattgc
    11161 agttcatgac gctgtttctg tttgaagtcg tatagatgta aatgccatgg ctcgtgcatc
    11221 tgtatacatt acagttcgag atgttgttga aggaagattt gaagttgtag ataccgtatt
    11281 ggccatttgt tatcgagaag ttactaatgt ttacctgtgt tgtaggtgtg gttatataga
    11341 agacatttcc ggttccactg ccatccacga gtacgccatc tctactttca ccaatcaagc
    11401 taagcttttt gtttactatt acgtgatcat agtagattcc attatagaca tagactgtgt
    11461 cacctgctgt tgcgttggtt actccctcat gaatcgtttt tacgtgtgtc tgatcatacc
    11521 atcctggtga ttggtcattg tcaacataga tggtccctct tgttctcagg tttgtagtac
    11581 tatctgtttt ttctgcaacg attgtatctt ctctaggggt atcgctactg atagttccta
    11641 ttctattatt actgtttatg gcaatgttat tattaccatt ggatgttatt ttatttgttt
    11701 ctccgttagc aaatgcggac actggcatca caaaagccat ggtaatagcg attgctatta
    11761 cagccttgta tatctttatt tttttcatgt ttcactcact tctcctattt tattttttat
    11821 atattcgtag ggatttcgtt ctacttgctt aaatctcttc gtccctacct ttttactcaa
    11881 atttttccac agatataaat ataaatcaaa gattatttac gaaatatata atgctgataa
    11941 gatatataaa tgttgcgtaa taatgccaac tattcggtaa aactggttag ggcgtgtaac
    12001 atttgtaaca taaaatggta tggctacttt aaaatccatt tattgatact tccggtgaaa
    12061 aaatatggga ttatcaatcg ggagacttct tttatgattc tcctgctatt gctgatggaa
    12121 tggtatacgt tgactccttt gatcataaga tgtactgtct cctcacggtc accgcggagg
    12181 ttccagaaag tcaaacggca cgatcacaaa atgggacgat ccttttgttc ttcatcagac
    12241 tctgaagcct ggcaacatct tcgagatcat cgtacaatcc ttcaaaggaa acctatcttt
    12301 tttttccgat atctatcatg actccttgat gagagcatta ctctttggtt ctaacaaaga
    12361 atggaatgga gaatatgaaa atcccaccaa gtagaagagt catcccaaca aacacaaaca
    12421 caggattcat gagtcgtatt ataggaataa gaccaaggat catgcacagg aatccgatac
    12481 tgtgtatgat gtattttccg taggtttttc tactgaaatc ttgaagtgat ttccagacga
    12541 cagcataaaa ccatgcgata aatccttccg tacctatgat acttattctg atttcaccat
    12601 tattccatgt tttacttcaa atcccctcta ttcaaaccac ttttaatatc cttgtccctg
    12661 attttatctc cacagaaaca tttaaacatg gggtatatct atagtaagcg taaggaggaa
    12721 atcatgatga aaaatcgaat agagaacaaa atatttgttt ttgggctgat gatattcgtt
    12781 atcggagctg tgtatcttcc aagtatcggt gcagagatcg gaagaaatac tcctaccatg
    12841 agtgactctg gatatcgctc tgcatcgact accgattggt ggatgatgtt ccgccatgat
    12901 gcgagccaca gcggatattc cagttcttcc gcaccgcaga ccaacaatgt gcagtggacg
    12961 tactcggtcg ggttagaaat gagcgggtct cctgcggtcg ctgatggcaa ggtgtacatc
    13021 ggttcatggg acaagaagtt ctattgtctg gatgctacca ccggagtgaa actctgggag
    13081 tatctgacag ggcttgagat aacctctacc ccagcgataa tgaacggcaa ggtgtacatc
    13141 ggctcaaacg atggcaaggt gtattgtttt gatgcaaaca cgggagtaaa actctgggag
    13201 tacctcacgg gtgcagatgt cgtgtcctca ccaacgattg ttgatgggaa cgtctacgta
    13261 ggctcacggg atcacaacgt gtactgcctg aacgcggaga ctggtgcgaa cgtctggaat
    13321 tataccaccg gtgatgacat tatttcttcc cctgcggtgt ctgacggaaa ggtgttcatt
    13381 ggttcctggg atgataaagt gtactgcttg gatgcaacga caggggcgtg gatttgggag
    13441 tacacgaccg gcaatgatat ctattcttcc cctgcggtcg tcgataataa agtctatatc
    13501 gggtccagcg acaataagct gtactgcttg gatgctctga gcgggtcgca tctctgggat
    13561 tatacgacag gagcctcgat tgaatcgtgc ccagcggtaa caaacgggaa ggtctacgtt
    13621 ggttcctttg acaacaaagc atattgcctg gatgcttcga ctggtgaaaa aatatgggag
    13681 tatctatcgg gagacttctt ctatgcttca cctgctgttg ctgatgggaa ggtatacctt
    13741 ggctcctttg atcataagat gtactgcctc aatgcggata ccggggaaaa gatatggagt
    13801 tataccactg ggtatatcgt gtggtcctcc cccgcggtcg cagacgaaaa agtgtacatc
    13861 ggctcaggtg accacaaagt ctactgtttt aaagacgcca atgctgcccc agaaatacca
    13921 gctgtaccta ccggtccatc tataggacaa aaaggagtga actacacgtt ctcaactgta
    13981 acgacagacc ctgaaggaga ccaagtgttc tatttgtttg actggggcga tggaacaaac
    14041 agcaactggg taggacccta caactcaagt gttcctggaa ccacatccca tgcatgggct
    14101 gacgcaggta acttctcgat caaagcaaaa gcaaaagaca ccaatggtgc agaaagcgac
    14161 tggtctgcaa ctcatagcat ctcgattacc ctagggtcga ttcttgagat ccaagacatc
    14221 agcgggggac tgtttaagat aaccgcagtg ataaagaaca ttggcgatac cgctgcaacc
    14281 aatatcagct ggagcatcaa ggtcgtcggt ggtgcctgga tcggaaaaga taccacagga
    14341 gatatcaata ctcttgctgc tggtggacaa caaaagat
//
LOCUS       DATMXJ010000141         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_39473, whole genome shotgun sequence.
ACCESSION   DATMXJ010000141 DATMXJ010000000
VERSION     DATMXJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39473"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VN377_03840"
     CDS             <1..515
                     /locus_tag="VN377_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWR27553.1"
                     /translation="VKIVKSRAAKKIIFVPDRNLGLYIQKQVPEKEMILWPGVCRIHH
                     DIKKDDVLLMKQKHPDAEFLAHPECQPEIIELADYAFSTNGMVEHVKTSPKKEFIIGT
                     EKGLCYRLEKENSGKKFYPIPSAVCPNMKKTTLEKVLKSMETLEPKIHLSETIMRKAR
                     VPLQRMIDMK"
     gene            527..1696
                     /gene="nifS"
                     /locus_tag="VN377_03845"
     CDS             527..1696
                     /gene="nifS"
                     /locus_tag="VN377_03845"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HWR27554.1"
                     /translation="MKRIFLDHASTTKVDEEVIHTMLPYFSQYFGNPSSIHAFGREAR
                     EAIDSARVHVSDILGARDDEIIFTAGGTESDNLAIKGIAYLNKEKRTMNGPHIITSTI
                     EHPGVLEVCRHLETQGFQVKYLPVDNQGLIDLETLRASISKNTFLFSFMFANNEIGTI
                     EPIEEIGKIAKEHEITFHTDAVQAVTKVPIDVKKQHIDMLALSSHKIYGPKGIGALYI
                     RNGIKIQPIIHGGGHEKGMRSSTLNTPGIVGLGKACELAKKRMTTDNSRMKTLRDNLI
                     KNILQIEESYLNGHPEKRLVNNAHFRFTGIEGESLLLGLDEQGIATSTGSACSSKKLQ
                     ASHVLLAIGLDPVQAHGSLRLSLGRENTKEEITYVSKVLPQIVERLRTMSPLWNR"
     gene            1700..2386
                     /gene="queC"
                     /locus_tag="VN377_03850"
     CDS             1700..2386
                     /gene="queC"
                     /locus_tag="VN377_03850"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWR27555.1"
                     /translation="MTKKAVCLISGGLDSAVTAFIAKKQGYDIYALSFRYGQRHAKEL
                     ACAKKISKAVGVKNHIILSIDLQKFGGSSLLSQSTEKITDHRVKDIGKHIPSTYVPAR
                     NTIFLSYALAYAETIDADAIFIGVNAVDYSAYPDCRPKYVKTFQQLAHLATRQGVEGK
                     TVRIKAPLLHLTKAEIVKKGLKLNVPFSKTWSCYRGEASACGRCDSCLLRLKGFQEAN
                     VKDPVPYAIK"
BASE COUNT          833 a          559 c          582 g          631 t
ORIGIN      
        1 gtgtgaagat tgtaaaaagc agagctgcaa aaaaaatcat ttttgtacct gaccgcaacc
       61 ttgggttgta tatccagaaa caggtcccgg aaaaagaaat gatcctctgg ccaggggtct
      121 gtcgcatcca tcacgatatt aaaaaagatg acgttttact aatgaaacag aaacaccctg
      181 acgcagaatt tctcgctcac cctgaatgcc aaccggaaat catcgagctg gcggattacg
      241 ctttttctac aaatgggatg gtggaacatg tgaagacgtc accaaaaaag gaattcatca
      301 tagggaccga aaaagggttg tgttaccgac tggaaaagga aaactcgggg aagaaattct
      361 atccgatccc ttcagccgtg tgccccaata tgaaaaaaac caccttggaa aaagtattaa
      421 agagtatgga gactttggaa ccaaagatcc atttgtcaga aacgatcatg cggaaggcac
      481 gtgtcccatt acaacgcatg atcgatatga agtaaggtga acgagaatga aacgtatttt
      541 cctggatcat gcatctacga cgaaagtcga tgaagaagta attcacacaa tgctcccgta
      601 tttctcacaa tattttggga acccatcgtc gattcatgcg ttcggacgtg aagcccgcga
      661 agcaattgac tctgcaaggg tccatgtcag tgacatcctt ggtgctcgtg atgatgaaat
      721 catctttacc gcaggtggaa ccgaatcaga caaccttgcg ataaagggga ttgcctattt
      781 gaacaaagag aaacgaacaa tgaatggacc acatatcatc acatcgacga ttgaacatcc
      841 tggtgttctt gaggtctgcc ggcatctcga aacacaagga ttccaggtca aatatctccc
      901 agtggataac cagggtctga ttgatctgga gacacttcga gcgtccattt ctaaaaacac
      961 cttccttttt tcattcatgt ttgcgaataa tgagattggc accattgaac caatcgagga
     1021 gatcggtaag atagccaagg aacacgagat taccttccat accgatgcgg tccaagcagt
     1081 gacgaaggtt ccgattgacg tcaaaaaaca acatatcgat atgcttgcat tatcctccca
     1141 taaaatctat gggccaaaag gaataggagc actctatatt cgcaatggga tcaagattca
     1201 accaatcatc catggaggag gacacgaaaa aggcatgcgg agcagcaccc tcaacacccc
     1261 tggtatcgtc ggtcttggaa aagcctgtga gctagcaaaa aagagaatga caaccgacaa
     1321 tagcagaatg aaaacattac gggataatct gataaaaaat attttgcaga tcgaggaaag
     1381 ttacctcaat ggtcatcctg agaaacgatt agtgaacaac gcacatttcc gcttcacagg
     1441 aatcgaagga gaatctcttc ttcttggctt ggatgaacag gggattgcga catcgaccgg
     1501 ttctgcatgc tcgtctaaga aactccaggc atctcatgtg ctgcttgcga ttggcctaga
     1561 cccggtgcaa gctcatggat cactgcgact ctcacttggt cgggagaaca caaaagagga
     1621 aataacgtac gtttcaaagg ttcttcctca gattgttgag agactccgta cgatgtcgcc
     1681 tctgtggaac cgataaggta tgaccaaaaa agcagtgtgt ttgatatctg gtggtcttga
     1741 ttccgcggtg actgcgttta tcgcaaaaaa acagggatac gacatttatg cattatcgtt
     1801 tcgttacgga caacgacatg caaaagaatt agcatgtgca aaaaaaataa gcaaagcggt
     1861 gggcgtaaaa aaccacatca ttctatcgat tgaccttcaa aaatttggtg ggtcttcgct
     1921 tctttcacag tctactgaaa aaatcacaga tcatcgggta aaggatatcg ggaaacacat
     1981 tccatcgacc tatgttcctg cacgaaacac gattttcctt tcttatgctc ttgcatatgc
     2041 tgaaaccata gacgctgatg cgatcttcat cggagtaaat gccgtcgatt actcagcgta
     2101 tcctgattgc aggccaaagt acgtaaaaac gttccaacag ctggcgcatc ttgcgacacg
     2161 acagggggtt gaaggaaaaa ctgttcgtat caaagcacca cttctgcatc tgacaaaagc
     2221 tgagatagta aagaaagggc tgaagctgaa tgtccctttt tcgaaaactt ggtcatgtta
     2281 ccgaggagag gcatcagcgt gcggtcgctg tgattcctgt ctattgcgtc tcaaagggtt
     2341 ccaagaggca aacgtaaaag accctgtacc ctatgcaatc aaatgaaaca cagtggatat
     2401 atctctttat tacataactc aagttcattt taggaaaatt tattaatacg ggacaacact
     2461 tttcaagcct gatggtgcga gagagaaaaa cacgatttaa agatattgat aagttgcatg
     2521 agataataga tagcgcctcg ttaccaaaac acagaacagt tacctcgtat gatgctggaa
     2581 aacttgactc gttgcgaaaa cgatt
//
LOCUS       DATMXJ010000142        12133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7860, whole genome shotgun sequence.
ACCESSION   DATMXJ010000142 DATMXJ010000000
VERSION     DATMXJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 12133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12133
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7860"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VN377_03855"
     CDS             <1..1054
                     /locus_tag="VN377_03855"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27556.1"
                     /translation="DGSGNAYVTGVFLVSITFGTTTLTSQGSWDVYVVKLDGNGDWQW
                     VVSGGSSSTEGAWGTAVDAKGDPYITGVFEGSVTFGESTLTTQGGRDVFVAKLDADGN
                     WLWAISAGGADWDEGYALTVDSSGYVYATGYFDGSALFGETTLTTQGDYDVFIAKLSH
                     DGVNQPPVADFSYSPEKPVVGQMVTFDASHSHDPDGDIVSYVWDFGDETAGTGMTADH
                     VYSGPGSYDVSLTVMDRESLEDTLIKQIEVSDVVVEINFSINSGLGVNVVITNSGAED
                     ATGVPWSIHVEGGMVGFIKKTRDGTLDVPAGGSATVGTGIFFGLGPIMITARVGAEEK
                     TAAGTQVIIFSMVTDG"
     gene            1149..3335
                     /locus_tag="VN377_03860"
     CDS             1149..3335
                     /locus_tag="VN377_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HWR27557.1"
                     /translation="MVKKAVVFVVIGVLVGMSVFPSTGTLVSEKPVNTAGQKTNGAPA
                     FWETVSQLGFQTRTLERTFVSPGINFPLPGEIFRVGDVVEINGTASLPEFQNFILEWG
                     VGLSPTEWFTTGVALIANGSVEIMNATLGFWDTGAVTEAGYYTIKLTVHLTDSGQYSV
                     NVSIYLDPTLHLNFPFGWRHGILGSQVAIWSPIALSDINSDGYQEIGFGTVTVVPAGD
                     NNYDYVLDHNGNVLQGWPLQMYSIQGASLTFADIDRSTSTEEVVGGMWGDDIFVWHDD
                     GTVVDGWPKPVAASRSSAAVIDLDGDGDLEIILPSTDGGGKIYALHHDGTMVDGWPVT
                     IGSPLRSAVATADIDRDGFPEIVCGDQNGYVHAFHHDGSVVDGWPQQAHDFIKSSPVI
                     ADLEGDGDLEVLICSGFTEQRIISAWHHDGTMVAGWPVENGLSFAQPSVADLDGDGDL
                     EILSGGAIPDTPYGRFYVWHHDGTVVDGWPITFAWDWSQYLDYIYAQPVVGDIDGDAD
                     VEIIAGSYAKKLYAWHHDATNVTGWPKRIGDAVDATAAIADIDNDALVEVAVAGDDGK
                     IYVWDMPGIYNSSHMEWPLFQYDRHHTGCYGSVPGSNQPPAAPTITGPAKGKIKVATE
                     YNFTTVDPEGDMVYYFIDWGDSSNSSWIGPAPSGDVVPQSHTWSKKGTYIIKAKAKDI
                     YGNESDWGTLSVTMPCSYNIPFQQFWELLFERFPNAFPIVRHLMGY"
     gene            3469..4530
                     /locus_tag="VN377_03865"
     CDS             3469..4530
                     /locus_tag="VN377_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27558.1"
                     /translation="MSKKSLIVKWVVVGIILLFVGTSLSPSATLAQTFDKNIITVDDE
                     PGDADFTSIKEAVNSSNPGDTIEVYSGTYPEDGILLRKDNVSLLGVSHELGEGNDSGQ
                     PFIKGNGIDTVIKIDASHVIVSNFTIENPETETLFTAGIFVNHIPGYEQNNITISECV
                     ITNSSHTGIYVTYTRDNIRIIGNHISHCFLHGIAIMAYSPVSGFVIIGNVITECRDSG
                     ISFDGHDHQNVSFNRISNCKMGIEFGADNTSVYGNDIENCSLGIRCGGVRNIFTKNNF
                     KNYSRIGFWFQKYLLWPLKNKWIGNYWDTWKGSGPKIILGFVYLDLYGEWIQFIFLFP
                     WVEFDWHPAKEPYDIPGMS"
     gene            complement(5065..5436)
                     /locus_tag="VN377_03870"
     CDS             complement(5065..5436)
                     /locus_tag="VN377_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013135785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HWR27559.1"
                     /translation="MNKNEIPFHVIDWNLIPKTEHKGEKGQAFWQTHQLPGVRIRIVE
                     YTAGYLADHWCKKGHIVHCLKGDFTSELQDGRTFHLKEGMTYVVSDDISSHRSTTDHG
                     VTLLIIDGDFLNADSPNKKIG"
     gene            complement(5456..6262)
                     /locus_tag="VN377_03875"
     CDS             complement(5456..6262)
                     /locus_tag="VN377_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWR27560.1"
                     /translation="MSQHNMKIRLYQVDAFTDAVFHGNPAAVCILDDWLDDRLMQQIA
                     MENNLSETAFAVQKNKQYHIRWFTPETEVTLCGHATLATAHVLFTHYHYPGNEITFKS
                     RQHGILKVRKEADTLALDFPADTIKKATPPPGLIKSLGKKPQEVWKGKTDYLLIYRTE
                     KDIRTISPNYHLLTLVKARGTIITAPGDTTDFVSRFFAPQVGINEDPATGSAHTTLIP
                     YWAEHLNKKELTAKQLSKRQGLMNCRLENNRVSISGKATTYLEGEIINLS"
     gene            complement(6410..7327)
                     /locus_tag="VN377_03880"
     CDS             complement(6410..7327)
                     /locus_tag="VN377_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HWR27561.1"
                     /translation="MNEMASTVEKIFEKKNGVITNFFTGFIRIRGVLNWFAISFFGFL
                     LGISSLDITSHIVPLFFFVVSTFFILAFTFAINNYYDIDTDKENPRRAHLNAMASGKI
                     TKHTGMMLNLIFIVIPLVLSLFYTPEVFVFCAVLILWMWMYSAPPLRLKGRPGLDILW
                     HFIAFLALLLWGSYLAGSVSQMTLLIAISLGIFGCFAQIDNHIHDYPFDKASGAKTFA
                     VWTGIHKATVALKVSFILYMVFLIPLIVLFSLYSPLTILIVCAGIVASALIIKIKKST
                     GTPTLYHVINFFGRSVYVTCLIYHLSSLL"
     gene            complement(7605..7853)
                     /locus_tag="VN377_03885"
     CDS             complement(7605..7853)
                     /locus_tag="VN377_03885"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HWR27562.1"
                     /translation="MNEDKHLLKELKAGTKDFEWLSKNYPALQKKYPKQFIAVKNQQV
                     IIAHKNLVTLINTLKKKFGNPNDFLIDFVPDENYTLVV"
     gene            8116..8358
                     /locus_tag="VN377_03890"
     CDS             8116..8358
                     /locus_tag="VN377_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27563.1"
                     /translation="MRIGSIKEYMCRDCKYFEYSGIYAGEGVCRASGYKMKNEIPLFY
                     AGEEELKSKGEQIPVFFCKYHKKERIKKRTYEVKKR"
     gene            8418..8726
                     /locus_tag="VN377_03895"
     CDS             8418..8726
                     /locus_tag="VN377_03895"
                     /inference="COORDINATES: protein motif:HMM:NF042844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6794 domain-containing protein"
                     /protein_id="HWR27564.1"
                     /translation="MKKVGYSTLDIILEDILKTLSGDFKEKVKEMTCDEFIIKQHFLL
                     GMTIKEKYFYKNKAQEHLIKSLGVGNKKEYRFLDGDVFSQIILEALWKKITTESKKQL
                     "
     gene            complement(8747..9169)
                     /locus_tag="VN377_03900"
     CDS             complement(8747..9169)
                     /locus_tag="VN377_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27565.1"
                     /translation="MKCPLYVDFTRMCLVKFPTLVKFTMLQTCASEHHVDCPIYQVHD
                     SDFYCKYFYDCANQYIENTPKILQDIFLNKNMFEVLKNIWTNYCLIPENSKICAKYQL
                     YSKGEIPPITLMPDGEEISSFDLLFRRKIIITPPDKTI"
     gene            complement(9894..10781)
                     /locus_tag="VN377_03905"
     CDS             complement(9894..10781)
                     /locus_tag="VN377_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWR27566.1"
                     /translation="MVSVKKKIINGNTYYYLEHSFRKNGKINKKEKYLGPTLPKNIEK
                     IKEEFQKEFYHETWFNRFDIIKEQFIKEKRNIPLSLEKKQTEQFATTFTYNTNRIEGS
                     TITLRETADLLERGISPSRRPIDDIKETEAHKKVFTDMLSYKKEIALPTVLYWHQQLF
                     QDTKPDTAGKIRQHPVGISGSKFLPPYPIELDFLLRDFFDWYHENKKTLHPVQIAGLT
                     HLKFVTIHPFGDGNGRLSRLLMNYVLYKNRYPMLIIPYSQRESYYTALERSQLKKDEN
                     IFILWFFKRYIKEYKQYLI"
     gene            11363..>12133
                     /locus_tag="VN377_03910"
     CDS             11363..>12133
                     /locus_tag="VN377_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27567.1"
                     /translation="MIDEDNLPEAIFDYTFKKYDGQIGGMCQKYVEEFPEKDCELPDE
                     DWHKNFLCWLIYEKVLPQTGKTIAEEFAEQSSEMTSEMKHQALSMRHVIRSDFQIVSE
                     KGVMVQFKDVHTKKIYPVKRYKNGPRYPLNTVVTGRIFPFGDHYRTTGFFFYREDPFL
                     TLSELIMNEYDNDLIARIENIQLRIGSSFMSVMMSYPAHWIDWMCEHYQIQQRLKKDK
                     IHAIQQRLTSDIQSIFQGLPKEAQEVLRLCMKNGGVVKY"
BASE COUNT         3250 a         2098 c         3078 g         3707 t
ORIGIN      
        1 tgacggcagt gggaatgcct atgtcacagg agtgttcttg gtgtcaataa cgtttgggac
       61 gaccacgctt acgagtcagg gtagctggga tgtgtatgtc gtaaaactcg atggcaacgg
      121 agactggcaa tgggtggtca gtggtggaag cagctcaacg gaaggggcat ggggtaccgc
      181 ggttgatgcc aaaggagatc cctatatcac tggggtgttt gagggttcgg tcacctttgg
      241 tgaaagcaca cttacgactc aaggcggtcg tgatgttttt gtcgcaaaac tcgacgccga
      301 tggtaactgg ctctgggcga tcagtgctgg tggagctgat tgggatgaag ggtacgcgct
      361 taccgttgat agcagcggat atgtctacgc gacaggatat ttcgatggtt ccgctctgtt
      421 tggggaaacg acgctgacca cgcaaggtga ctatgatgtt tttatcgcga agttgtcaca
      481 tgatggtgtg aatcaaccac cggtcgctga tttcagttat tcacctgaaa aacctgtcgt
      541 tggtcaaatg gtgacgtttg atgcgtctca ctcacacgac cctgatgggg acatcgtctc
      601 ctacgtgtgg gatttcggag atgaaacagc aggaacagga atgaccgcag accatgtcta
      661 ttccgggcct ggttcctacg atgtgtcgct gacggtgatg gacagagaat ccttggaaga
      721 tactctcatc aaacaaatcg aggtgtctga tgtagtggtg gaaatcaact tcagcatcaa
      781 cagtggtctt ggggtgaatg tcgtcatcac gaacagcgga gcagaggatg ccactggtgt
      841 tccctggtca attcatgtgg aaggcggcat ggtaggattt atcaagaaaa ctagggatgg
      901 tacgcttgac gtccccgcag gaggatctgc gaccgtgggg actgggattt tcttcggact
      961 tggtccgatc atgatcactg cgagagtcgg tgctgaagaa aaaacggccg cgggaacgca
     1021 ggtcatcatt ttctcgatgg tgaccgatgg gtgactcttg catcgccggt ggtgatgtgg
     1081 ttcccgaacg cttttctatc attacggcgt atactcgata cgtaccgtgt agaatttggg
     1141 gtgaatagat ggtgaaaaaa gcagtcgttt ttgtcgtcat tggcgtactt gttggcatga
     1201 gtgttttccc atcgactggg actttggttt cagaaaaacc tgtgaacact gcaggacaaa
     1261 aaacaaatgg agctcctgca ttttgggaga cggtatcaca gcttggattt cagacaagaa
     1321 cgcttgagcg tacgttcgta tcaccgggca tcaattttcc tttaccaggg gaaatctttc
     1381 gggtagggga tgtcgtggag atcaatggta ctgcatccct gccggagttc caaaacttta
     1441 tccttgaatg gggggtcggg ctgagtccga ctgaatggtt taccaccggt gtggctctca
     1501 ttgcgaatgg gtccgttgaa atcatgaatg cgaccttggg tttctgggat accggtgcag
     1561 tcacagaagc agggtattac accataaaac tcactgttca tctcaccgat tcagggcagt
     1621 actctgtgaa tgtcagtatt tatcttgatc cgacgttgca tctgaatttt cctttcgggt
     1681 ggcgccatgg tattttgggg tcacaggtcg ctatctggag tcccattgct ctttctgata
     1741 tcaactctga tgggtatcag gagatcggtt ttgggaccgt gaccgtggta cctgctggtg
     1801 ataataacta tgattatgtt cttgaccata acggcaacgt actgcaggga tggcctcttc
     1861 agatgtacag tattcaaggt gcatcgctta cgtttgcgga tatcgatcgt tcgacgagta
     1921 ctgaagaggt cgtcggaggc atgtggggag atgacatctt tgtatggcat gatgatggga
     1981 ccgtggtgga tgggtggccg aaacctgtcg ctgcatctcg gagctcggcc gcagtaatcg
     2041 atcttgacgg tgatggtgat ctggagatca ttcttccttc gacggatggc ggtgggaaaa
     2101 tctatgcgtt gcatcatgat gggaccatgg ttgacggatg gcctgtcacc attggtagtc
     2161 ctctgaggag tgccgtggcg accgctgata tcgaccggga tggttttccc gagatcgtct
     2221 gtggtgatca aaatgggtat gtgcatgcgt tccaccatga tgggagcgtg gttgatggat
     2281 ggccgcagca ggcccatgat ttcattaaat cctccccggt catcgctgac cttgaagggg
     2341 atggcgacct tgaggtactc atctgctctg gttttaccga gcaacggatt atttccgcat
     2401 ggcatcatga tgggaccatg gttgctggct ggcctgtgga gaacgggctg tcgtttgctc
     2461 agccgtccgt cgcagacctg gatggtgacg gtgatctaga gatactctct ggtggtgcga
     2521 tcccagatac gccgtatggc cgtttttatg tctggcatca tgatggaact gttgttgacg
     2581 gctggccgat cacctttgcc tgggattggt cccaatatct tgattatatc tacgcgcagc
     2641 ccgtcgttgg tgatatcgat ggggatgctg atgttgagat catcgctgga tcctatgcta
     2701 aaaaattgta cgcctggcat catgatgcga ccaatgtgac cggctggcca aagagaatcg
     2761 gtgatgcggt cgatgcaact gctgcgattg cagatattga caatgatgcg ttggtggagg
     2821 ttgctgtcgc aggcgatgac gggaagatct atgtctggga catgcctggg atctataatt
     2881 cgtcacacat ggaatggccc ctgtttcagt acgatagaca tcataccggc tgctacggga
     2941 gtgtacctgg tagtaatcag ccgccagctg caccgacgat tacgggcccg gcgaaaggaa
     3001 aaataaaggt cgctactgag tataatttta cgacagtaga cccggaaggt gatatggtgt
     3061 actacttcat cgactggggg gacagctcaa acagtagctg gatcggcccc gctccatccg
     3121 gagatgtagt tcctcaatct catacctggt ccaaaaaagg aacctatatt ataaaagcca
     3181 aagcaaagga catctatgga aatgagagtg attggggaac attgagtgtg actatgccat
     3241 gttcgtataa catacctttc cagcagttct gggaactgtt gttcgaacgg ttccccaatg
     3301 cattcccaat agtacggcat ctaatgggat actgaatccc ccatcttttt ttctcatttg
     3361 aattcgtaca aaaaatgtct taaaaccgtg tcaatgcttc tgttgttaaa ggatattctt
     3421 tttataggag aactgtatta ttaaatagtg ataaaagggg gaaagatcgt gagtaagaaa
     3481 tctctgattg tgaaatgggt ggtggtcgga atcattctct tgttcgttgg aacaagtctc
     3541 agtccatctg caacattagc acagaccttt gataaaaata tcattacggt tgatgatgaa
     3601 cctggggatg cagattttac atccataaag gaagcagtga attcttcaaa tcctggcgat
     3661 acgatcgagg tgtatagtgg gacgtatcct gaagatggga ttcttctaag gaaagataat
     3721 gtgagtcttc ttggcgtatc tcatgaactc ggtgaaggta atgatagcgg acaacctttt
     3781 attaaaggga acggaattga tacagtcatt aaaatcgatg caagccatgt gattgtctcg
     3841 aattttacca ttgaaaatcc agaaactgaa actttgttta ctgctggcat tttcgtaaat
     3901 catattccag gttatgaaca aaacaatatt accatatcag agtgtgtcat tacgaattca
     3961 tcgcacacag ggatttatgt tacgtataca agagataata ttagaatcat tggcaatcac
     4021 atcagtcatt gctttcttca tggaatcgcc attatggcat acagccccgt tagtggtttt
     4081 gtcataatag gaaatgttat cacagaatgt cgtgatagtg gtattagttt cgatggccac
     4141 gaccatcaga atgtttcctt taatagaatt agcaattgta aaatgggaat agaatttgga
     4201 gctgacaata ccagtgtcta tggtaatgat attgaaaatt gttctctcgg gattcgatgc
     4261 ggcggcgttc gaaacatctt cacaaagaat aactttaaaa attatagtcg aatcggattt
     4321 tggtttcaaa aatatttatt gtggcctttg aaaaataaat ggataggaaa ttactgggat
     4381 acatggaaag ggagcggccc aaagataatt ctgggatttg tatacttaga tttgtacggc
     4441 gagtggatac aattcatatt cctctttcca tgggttgaat ttgattggca tccagcaaaa
     4501 gaaccctatg atattccagg gatgagttga atagagtctg tctgaaattg tcgatgatgc
     4561 ctgagaaaat agttattttg attcgcacga ccgctatagt aatttatcca aaaggcttga
     4621 aaaagaggac agtagacgca tatagacaat ggggaaaata tccgaggccg attaagattt
     4681 ctcggggccg tatagaccct ctcatttaag atattaacta atgttcaacg ggttatacgt
     4741 ttataaagaa agtgttatgg aacttcaggt ttcccgctgg agcatatggg ttattattgc
     4801 aatgagtgta aaaagaccat tgcagcggaa gagtttttcc atggatgaat caatcgcatt
     4861 gacaatgcat atttacaaat tttgtgcaca aaaataatgc atataataaa taatatgcat
     4921 ctcgttgggg agaagaacag gagattgtag tatcccaaat taacttccac ctgatttatt
     4981 aatgttataa attaccgaat ttggtcatag tatctttgta atactttttt ctaacttttt
     5041 ttgatgtctt ctgaatctct ggtatcatcc gatttttttg ttaggtgaat ccgcgtttaa
     5101 gaaatctcca tcgatgatga gtaaggtgac gccatggtcg gtggtggaac gatgggagct
     5161 gatatcatca gacaccacgt aggtcattcc ttcctttaga tgaaatgttc tgccgtcttg
     5221 tagttcactg gtgaaatcac ccttgaggca atggacgata tggcctttct tgcaccagtg
     5281 gtccgcgaga tacccggcag tgtattccac tatcctgatg cgtactccag ggagttgatg
     5341 ggtctgccag aaagcctgtc ctttttctcc tttgtgttct gtctttggga tgaggttcca
     5401 gtctatgacg tgaaagggta tttcattctt gttcattggt atgcaccttt gtcttttagg
     5461 agaggtttat gatttctcct tctaagtagg ttgtggcttt tccagagatg gatactctgt
     5521 tgttctctaa gcggcagttc attagtccct ggcgttttga gagttgtttg gcggtgagtt
     5581 cttttttgtt cagatgttct gcccagtagg ggatgagcgt ggtgtgtgct gagccggtgg
     5641 ctgggtcttc gttgatccct acttgtggag cgaagaatcg tgagacgaaa tcagtggtgt
     5701 ctcctggtgc ggtgatgatg gttcctcgtg ccttgaccaa tgtgaggaga tggtagtttg
     5761 gtgatattgt ccgtatgtct ttttctgttc tgtagatgag caggtaatct gttttgcctt
     5821 tccagacctc ttgtggtttt tttcccaggc ttttgatgag gccggggggt ggtgtggctt
     5881 tttttatggt gtcagcggga aagtctaggg caagtgtgtc tgcttctttt cttactttga
     5941 ggataccatg ttgtctgctt ttgaaggtga tttcattgcc agggtagtgg tagtgggtga
     6001 agaggacgtg ggctgtggcg agtgtcgcat gtccgcagag ggtgacttct gtttccgggg
     6061 tgaaccatct gatgtgatat tgtttgtttt tttggactgc gaacgctgtc tcggataggt
     6121 tgttttccat ggcgatctgt tgcatgagtc ggtcgtcgag ccagtcgtcc aggatgcata
     6181 ctgctgcggg gtttccgtgg aagacggcat cggtgaacgc gtctacttgg tagagtctga
     6241 ttttcatgtt gtgctggctc aaggtttttc gtgtatttaa aaccattttt tttgttatgt
     6301 tggatttttt ttaagaaaaa aggaagaaat gtttactgac tcatcctgag gagtgtgaac
     6361 aggtcgcttt tcagagtttt ccttcatctt ctgactacgt cggttcttgt tagagtaggc
     6421 tgctgagatg gtagatgagg caggttacat agactgatct gccaaaaaaa tttatgacgt
     6481 gatacagagt tggtgttcct gtgctctttt ttatttttat tatgagtgcg cttgcgacta
     6541 ttcctgcgca tacaatgagg atcgtgagag gtgagtacaa agagaaaaga acgatcagcg
     6601 ggatgagaaa gaccatgtag aggatgaatg atacttttag ggcgactgtt gctttgtgta
     6661 tcccggtcca gaccgcgaat gtctttgccc cgcttgcttt atcgaaagga tagtcatgga
     6721 tatggttgtc gatttgtgcg aaacagccaa agatgcctag cgatatcgcg attaagagag
     6781 tcatctggga gacgctgccc gctagatacg agccccagag tagtaaggcg agaaacgcga
     6841 tgaaatgcca caggatatcc agtcctggtc tgcctttcaa tcgcagtggg ggagcggaat
     6901 acatccacat ccaaagaatc agtaccgcac agaacacaaa gacctctggt gtatagaaca
     6961 gggataggac caaggggatg acgatgaaga tgaggttgag catcatccct gtgtgttttg
     7021 ttatttttcc tgatgccatg gcgttcagat gtgctcttcg tgggttttct ttgtcggtgt
     7081 cgatatcgta atagttgttg atggcaaacg taaaagcaag gatgaaaaac gtggagacca
     7141 cgaaaaaaaa taatggtact atatggctgg tgatatccag ggaagagatc cctaagagaa
     7201 acccgaaaaa ggagatcgcg aaccagttaa ggactcctcg gatcctgatg aatcctgtga
     7261 aaaaattagt gataacacca ttcttttttt caaagatttt ttctacggtc gatgccattt
     7321 cattcatgga acgagagatc ggtttatacg agtattgatg aagcatcgtg caactagggt
     7381 tgatgaaaca gataatcctg atggaattaa taattgataa atgaatggaa aaagtagagg
     7441 gcgttgaaga aggagaaacg ggtgtatata aataagttga aggaaaaata atgtttcgat
     7501 aatgaagggt tatttttcaa ggtatacttc ttttctcgtg atattgtatg ctatttcttt
     7561 gaagaaacca ttaaggcgca taaggatatt cctccctgta tttcttacac aacaagagta
     7621 tagttttcat caggaacgaa atcaattaag aaatcgttgg ggttgccgaa ttttttcttc
     7681 aacgtgttga taagggtgac aaggttttta tgtgcgatga tcacctgttg atttttcact
     7741 gcgataaact gtttcgggta tttcttctgc aatgcaggat agtttttact gagccattca
     7801 aaatcttttg ttcctgcttt gagttctttg aggaggtgtt tgtcttcatt cataggcgtc
     7861 gtctcttttt taataaggaa tcatctttta ttatcttttt cgttttcaat gaatgtattt
     7921 cttaacgatt actcccagat gtcttgacat cttttcataa ggaatctgtt gtttaaatct
     7981 tttttgtagt atgtatttaa agaaggaggg tctgttcgga gttctacatg ggatgaagga
     8041 gaaacgagaa gagcggccag gcaaggaaaa aacgtaaaaa gtgacatggg ataggtgtaa
     8101 aggagatttg aaaacatgag aatcggaagt ataaaggaat atatgtgtag agattgtaag
     8161 tattttgaat actcaggaat ttatgctggt gaaggtgtat gtcgtgcaag tgggtataaa
     8221 atgaaaaacg aaatcccgct tttttatgca ggggaggagg agttaaagag taaaggtgag
     8281 caaataccag tatttttttg taaataccat aaaaaggagc ggataaaaaa aagaacgtat
     8341 gaggtgaaaa aaagatagtt ttcaggtggt ttatagagaa ctatttcttt tgatattact
     8401 atggatggga aatacaaatg aagaaagttg gctattcgac attggatata attcttgagg
     8461 acattctgaa gacattatca ggggatttca aggaaaaagt aaaagaaatg acctgcgatg
     8521 aattcatcat aaaacagcat tttcttttgg ggatgaccat caaagaaaaa tatttttata
     8581 agaataaagc acaagagcat cttatcaaga gtttaggtgt aggtaacaaa aaagagtaca
     8641 ggtttcttga tggagatgtt ttctcacaaa tcattttgga agcactttgg aaaaaaatca
     8701 cgacggaaag caaaaagcag ttatgagatg aaaagactga tcatagctat attgttttgt
     8761 ctggtggtgt tatgataatt ttccgtctga atagtaagtc aaatgaactt atttcctcac
     8821 catctggcat taaggttata ggtggtattt cccctttgga atagagttga tatttcgcac
     8881 agattttcga gttctctggt attaaacagt aatttgtcca gatgtttttc aggacttcaa
     8941 acatattttt gttcaaaaaa atatcctgta atattttcgg tgtattttcg atatattgat
     9001 ttgcacaatc ataaaaatac ttgcaataaa aatcactatc gtgtacttga taaatcggac
     9061 aatcaacatg gtgctctgat gcgcaggttt gtaacattgt gaacttgact agagtaggga
     9121 atttgacaag gcacatccgt gtaaaatcaa catagagtgg acatttcata tttcttgttt
     9181 tattttccat atcgttcata tcgtttccca tccgcctttt tatttcgaag atctcatgtt
     9241 ctttttacat tcacgattca tttacgtaaa cagtacaata taataattgt ttctttaaaa
     9301 atttatcttt ggtagagtat ttgtttatca ctgaagctgt ggtgaagttc ctagattaac
     9361 gaacttttca gaccgaatat tcctcatggt aaaatgaatg attatgcaag gttaagtttt
     9421 tagatgtgta ggtttttaaa gagagaaatg ttttaaaatt gttgcgggta gcttctgacg
     9481 gattggatat ccgttgattt tgaaacctgg gataaggtca ttaaaaaaag aggatggcgg
     9541 gaggtatagt gttactatct caggggatga gttgaaaaac agttcttcaa aacggaccaa
     9601 gaaataagga tagatcggag tattaaagat tcatcgcttt aattatggct gctatgtttt
     9661 ttgtttgtac cgatgcgtac aatattattt ccaactcctg agttaatgta tcataggtgg
     9721 tatgattata caacacaaat ctaacccagc aaggtatatt catatttgtc ctaaaactca
     9781 tctgatcata atgtatgatt ggagtctgga gggggttcgt agggagaaaa acggaccagt
     9841 aatcataaca tgtgatgagt cagctaatta aaaaaaaata gaaattgagg gaattatata
     9901 agatattgtt tgtattcttt gatgtatcgt ttgaaaaacc acaggatgaa aatattctca
     9961 tcttttttca gttggcttcg ttcgagggcg gtgtagtagc tttctcgttg tgagtacggg
    10021 atgatgagca ttgggtatct attcttgtat agcacataat tcatgaggag tcgtgagaga
    10081 cgaccgttgc catctccgaa tggatgaatc gtgacgaatt taagatgggt gagtcctgcg
    10141 atctgcacgg ggtgaagtgt ctttttattc tcatgatacc agtcaaagaa gtctcgtaat
    10201 aagaaatcga gttcaatggg gtatggcggg aggaatttac ttccggagat tcctacgggg
    10261 tgctgtctga tttttcctgc ggtatccggt tttgtgtctt ggaagagttg ctggtgccaa
    10321 tacagaacag ttggtaaggc gatttctttt ttgtaggaga gcatatcggt gaagactttt
    10381 ttatgtgcct ctgtttcttt gatgtcgtct atcggtcgtc gtgatggcga tatccctcgc
    10441 tcgaggagat ctgcggtttc tcgaagtgtt atggttgatc cttcaatcct gttcgtgtta
    10501 tacgtgaatg ttgtcgcgaa ttgttcggtt tgtttttttt ccagagatag tgggatgttt
    10561 cttttttctt ttatgaactg ttctttaatg atgtcgaatc tgttgaacca tgtttcgtgg
    10621 tagaattctt tctggaactc ctcttttatt ttttcgatgt ttttcggaag ggttgggcct
    10681 agatattttt cttttttatt gatcttaccg ttttttctga agctgtgttc gagataatag
    10741 tacgtgttgc cattgatgat ctttttcttt actgaaacca tacataggca ttacccctac
    10801 aaattaataa acttatctat ttttagtaaa atgtaggggt aaactaaaca tgaatcagga
    10861 gggggttctc ccatggagat ttcattgtga ttggagattt ttttgcccat caaagaatca
    10921 tgatgagaga atgataggta aaatctgtgg cattgttgaa acagacggtt caggtgagat
    10981 taacaagcca taactaaata aaaacagtgc tggacgcagg aaaaaaaata acggttttag
    11041 ataaggaaga taacggagaa atgagagtgc gtacctttca atgcttgatg agggagtgtt
    11101 tctgctgatc attactgcat agtattgcgt attgcaatga gcaatattca tctttcgcgt
    11161 aataggaaag aagagtaatt gtttgtgtat gtgtggtgat ggatctctag agtttgtctg
    11221 tggaggaact atgcctatcg gtgtacggag atttttattg attattcggc cgtcatggct
    11281 atcagacgat acatctttat taagagatac gcatacaaag aatgtaggat ccatggcagg
    11341 agcagttggg taggtggttc gtatgatcga tgaagataat ttgccagaag cgatttttga
    11401 ttatacattt aagaagtatg acgggcagat tggagggatg tgccagaagt acgtcgagga
    11461 gttcccggag aaggactgtg agttaccaga tgaagactgg cataaaaatt tcctctgttg
    11521 gttgatctat gaaaaagtgt tgccgcagac cgggaagacg atcgcagagg aatttgcaga
    11581 acagtcatct gagatgacct cggagatgaa gcatcaggct ctctcgatga ggcatgtgat
    11641 ccgctctgat ttccagatcg tatcagagaa aggcgtcatg gtacagttca aggatgtcca
    11701 taccaaaaaa atctatcctg tgaaacgata caaaaatggt ccgagatatc cgttgaatac
    11761 cgtagtgact ggccggattt ttccctttgg tgaccattat cgcacgaccg gtttcttctt
    11821 ttatcgggaa gacccgttcc ttacactctc agaactcatc atgaatgaat atgacaacga
    11881 cctgatcgct cgaatcgaga acattcaact gcgtatcggt tcttcattta tgtctgtgat
    11941 gatgagctac cctgctcact ggatcgattg gatgtgtgag cattatcaga tacaacagag
    12001 gctgaagaag gataaaatcc atgctattca acagcggttg acatcagaca tccaaagtat
    12061 ttttcaaggt ctgccgaagg aagctcagga ggtgttgcgg ctctgcatga aaaatggggg
    12121 ggtcgtgaaa tac
//
LOCUS       DATMXJ010000143         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_65586, whole genome shotgun sequence.
ACCESSION   DATMXJ010000143 DATMXJ010000000
VERSION     DATMXJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65586"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /gene="infB"
                     /locus_tag="VN377_03915"
     CDS             <1..1518
                     /gene="infB"
                     /locus_tag="VN377_03915"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWR27568.1"
                     /translation="HHAFTTLRTRGGSLADLAILVVDINEGFRPQTHESITILRQYKT
                     PFIVAANKIDAISGWQRNNDVAKNRLENQRPMTKTLFETKLYELIGIIYDKGFESDLY
                     FNIKDFRKSIPIIPVSAKTGEGIPELLMVLVGLAQRFLEDQLASETGAGKGTVLEVKE
                     DVGLGTTVDAIIYSGVLKKEDTIIFGTHDEPLVTKIKALLKPKPMDEIRDPRERFESV
                     KEVHAACGIKISAPDLDRVVPGAPLRVVHGDEIELIQEIKSQSQVNIELDKDGLYIKA
                     DTIGSLEALVKESREKGINIRKVEIGNISRRDIMETAAITNPLERVIFAFSVKILPEA
                     KEELLKSEVTIFNEDVIYTIIENYDLWLAKKKEELEKKRREDYIHPGMIRFLPEYVFR
                     ISHPAVFGVRVLGGRIKVDMRLIDQEGKSIGNIKGMQLENKPITEALQGAEVAISVEG
                     ITIGRQIKGGDIFFTDIPESDARKLQRMNVLNEDEKDVLNKIIDIKRKTNKFWGM"
     gene            1711..4143
                     /locus_tag="VN377_03920"
     CDS             1711..4143
                     /locus_tag="VN377_03920"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWR27569.1"
                     /translation="MIIAGLVILIAPSTMAAEAGGEANLVVPDLNTVEFFGINARLLL
                     GIGLIVCLLGLLFGLFNYLRVKRLPVHKSMRDISELIYETCKTYLITQGKFLLILWAF
                     IGAVIIIYFGLLLHFETFKVVVILSFSLIGIAGSYGVAWFGIRINTLANSRSAFASLR
                     GKPLPVNQIPLRAGISVGMFLISVELFMMLAILLFIPGSYAGPCFIGFAIGESLGAAA
                     LRIAGGIFTKIADIGSDLMKIVFRIKEDDARNPGVIADCTGDNAGDSVGPTADGFETY
                     GVTGVALISFIVLAINDPLIQVILLVWIFVMRVLMIVASSLSYVINDFIARARYAKAT
                     KMNFEAPLTSLVWITSFISIALTFIASYFLIPTLGGDTTLWWKLSIIITCGTLAGAVI
                     PEFVKVFTSTKSGHVKEVVTSSREGGPSLNILSGFVAGNFSAYWIGLSILLLMGIAFI
                     VSTLGLGSLMIPQAIIVGPAVFAFGLVAFGFLGMGPVTIAVDSYGPVADNAQSVFELS
                     LIEQIPNIKEEINNEFGFEPDFTHGKELLEEGDGAGNTFKASAKPVLIGTAVAGATTM
                     IFSIIILLTNKLDPTLIGNLSLLHPPFFLGLIAGGAMIYWFTGASTQAVSTGAYRAVE
                     FIKRNIKLEGVEKASVEDSKKVVEICTQYAQKGMFNIFIAVFCATLAFAFLEPYFFVG
                     YLIAIALFGLFQAIFMANAGAAWDNAKKFVEVDLKEKGTELHAATVIGDTVGDPFKDT
                     SSVAMNPIIKFTTLFGLLAVELSVSLTNQFGNGSSANPATLSLFVLFLAVSMYFVFRS
                     FYGMRIKTNDAQ"
BASE COUNT         1223 a          909 c          985 g         1232 t
ORIGIN      
        1 catcatgcgt ttaccacctt acgaactcgt ggcggctctc ttgcagacct tgcaatcctt
       61 gtggtggata taaatgaagg attcagaccg cagacccatg aatcgatcac tattctgcgt
      121 caatataaga cgccgtttat cgttgcagca aacaagatcg atgcaatcag cggttggcaa
      181 cggaacaatg atgttgcgaa gaatcgactt gaaaaccaac gccccatgac aaaaacactt
      241 ttcgaaacga aattatatga attaatagga ataatttatg ataagggatt cgaatcagat
      301 ttatatttca atattaagga tttccgtaaa agcatcccga tcattccagt ttctgcaaaa
      361 acaggagaag ggataccaga gttgcttatg gttctggtcg gactagcaca gcgattcctg
      421 gaagatcaac ttgctagtga aacaggtgct gggaaaggaa cggtcctgga agtcaaagaa
      481 gatgtcggct taggaacgac cgtagatgcc atcatttata gtggagttct caaaaaagaa
      541 gataccatca tcttcgggac tcacgacgaa ccattggtga ctaaaattaa ggcgttgctg
      601 aaaccgaagc cgatggatga aatccgtgat cctcgagaac gattcgaaag cgttaaagaa
      661 gtccatgcag cctgtgggat caaaatttct gctccagatc tagatcgtgt tgtccctgga
      721 gcaccattac gggtcgttca tggggatgaa atagaattaa tccaggagat caaaagtcaa
      781 tcacaagtga atatcgaact tgacaaagat gggttatata ttaaggctga tactatcggc
      841 tctctcgaag cccttgtgaa ggaatcaagg gaaaaaggaa tcaatatcag aaaagtagaa
      901 atcgggaata tctcccggcg agacataatg gaaacagctg cgataaccaa tccattggaa
      961 cgggttattt ttgcttttag cgtaaaaata ctcccagaag caaaggaaga attactcaaa
     1021 tccgaagtaa caatcttcaa cgaagatgtc atctacacaa taatcgaaaa ctatgatctt
     1081 tggttagcga aaaagaaaga ggagcttgaa aagaaacgtc gagaggatta tattcaccct
     1141 gggatgatta ggttcctccc cgagtacgtt ttccgtatta gccatcccgc ggtttttggg
     1201 gttcgtgtgc ttggtggacg tatcaaagtc gatatgcgat tgatcgatca agagggaaaa
     1261 agcatcggaa acatcaaagg aatgcaactt gaaaataaac ctatcacgga agcactccag
     1321 ggtgccgagg tcgctatatc agttgaagga attacaatcg ggagacaaat caaaggagga
     1381 gatatctttt ttacggatat ccctgagagt gatgcaagaa aacttcaacg gatgaacgtt
     1441 ttgaacgaag atgagaaaga tgtgttaaat aaaatcatcg atattaaacg gaagacaaat
     1501 aaattctggg ggatgtagaa ccttctctgt tgccgatatg gttatttttg tagaaacgac
     1561 atcttaaaat agaaaatact ggtttccggt tactacctta agaatggagg agtgtgttta
     1621 tcaatggaaa atcaattcaa aacagaacag tataaagaag acattggtaa taaatcaaga
     1681 cagaggattc ttcgatttct gtccgttctg atgattatcg cgggtcttgt tatactgata
     1741 gccccttcga cgatggctgc agaagctggt ggagaagcaa acctggtcgt ccccgacctc
     1801 aatacagttg agttcttcgg tatcaacgca agattgctgc ttggtattgg actcattgtc
     1861 tgtctacttg gattgctctt cggattgttc aattaccttc gagtaaaacg tcttccggtg
     1921 cataaatcca tgcgtgatat ctccgaatta atctatgaga cctgcaagac ctatctcatc
     1981 acacaaggca agtttctcct catcctatgg gcgttcattg gagcggtcat catcatttac
     2041 ttcggattac tccttcattt tgaaaccttc aaagtcgttg ttatcctttc gttcagctta
     2101 attggtatcg cgggcagcta cggggtagct tggtttggta ttcgcataaa tactcttgcg
     2161 aattctcggt cagcgttcgc aagcctgaga ggcaaaccat tgccagtgaa tcaaatcccc
     2221 ttgcgagcag ggatcagcgt cgggatgttc cttatcagtg ttgaactctt catgatgctt
     2281 gctattctcc tatttatccc tggttcctat gcgggaccct gttttattgg gtttgcaatt
     2341 ggagaatccc ttggagcggc agcattacgg attgccggag gaatctttac aaaaatagct
     2401 gatatcggct cagacctcat gaaaatcgtc tttagaatca aagaagatga cgcaagaaac
     2461 ccaggtgtta tcgctgattg cacgggagac aatgcagggg actctgtagg accaactgcg
     2521 gatgggtttg aaacatatgg cgtgacaggc gttgctctta tttccttcat cgtccttgca
     2581 atcaatgatc ctttgattca agtcatcctt cttgtttgga tttttgtcat gagggtctta
     2641 atgattgtcg ctagtagcct gtcttatgtg attaatgatt tcatcgctcg tgcacgctac
     2701 gcaaaagcca caaagatgaa ttttgaagca ccgttgacat cgttagtctg gatcacatca
     2761 tttatttcca tagcccttac ctttattgca tcctacttcc tcatccccac cctgggaggg
     2821 gatacgactc tttggtggaa actctcaatt atcatcacct gtggtacatt ggcaggagca
     2881 gttattcctg agtttgtgaa ggtgttcact tcgacaaaat ctggtcatgt caaagaggtg
     2941 gtgacgtcct cccgtgaagg cggtccatca ctgaatatcc tgtctgggtt tgtcgcaggg
     3001 aattttagtg cgtactggat tgggttgtcg attcttttac ttatgggaat cgcgtttatc
     3061 gtaagcacct tgggtcttgg aagtctgatg atcccccagg cgataatcgt aggtccagcg
     3121 gtgtttgctt ttgggcttgt cgcctttgga ttccttggga tggggccggt caccattgct
     3181 gttgactcct atggtcctgt cgctgataac gcccaatccg tctttgaact ttcattgatc
     3241 gaacagattc caaacattaa agaagagatc aacaacgagt tcggattcga acctgatttt
     3301 acccatggca aagaactttt agaggaaggc gatggcgcag gtaatacgtt caaggcctcc
     3361 gcaaaaccag tcctcattgg aaccgctgtt gctggggcaa cgaccatgat tttctccatc
     3421 atcattcttc taaccaataa acttgatcca acgctcatcg gtaacctttc gctcctccat
     3481 cctccattct tccttggact tattgctggt ggagctatga tttactggtt cacaggggca
     3541 tcaacacaag cagtctctac cggagcatac cgcgctgtcg agtttatcaa acgcaacatc
     3601 aaactcgaag gcgtcgaaaa agcatcggtg gaggatagta aaaaagttgt ggaaatctgc
     3661 acgcaatacg cccagaaagg catgtttaat atctttattg ctgtcttttg cgcaaccctt
     3721 gcctttgcat tccttgagcc gtatttcttc gtcggatatc tcatcgccat tgcgttattt
     3781 gggttattcc aggcgatctt tatggcaaat gctggtgcag cgtgggataa tgcaaagaaa
     3841 ttcgttgaag tagacctcaa agaaaaagga actgaattgc atgcagcaac agtgatcggt
     3901 gacacggttg gtgatccttt caaggataca tcatcggtcg cgatgaaccc aattattaaa
     3961 ttcaccacat tgtttgggtt gctcgcagtt gaattgtccg tctcattgac caatcaattt
     4021 ggaaacggaa gttccgcgaa ccctgcgacg ctctccttgt ttgtcttgtt ccttgccgtg
     4081 tctatgtatt tcgtgttccg gtcgttctac ggcatgcgga ttaagacaaa cgacgctcaa
     4141 taaaaacaaa aacgcctctc attatgatga gaggatattt tttttctttt tgttgttact
     4201 ttaaaaagag aaaaaagaaa atcaatgtat aaccgatgac cgtcattaac aaagagagga
     4261 tccatatcca aaacaagagt tttgcttttt tctcagggtc tttcaatgtt tcccgaaaac
     4321 ttcgtgtcat gattcacgct atcgttgtc
//
LOCUS       DATMXJ010000144         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_34025, whole genome shotgun sequence.
ACCESSION   DATMXJ010000144 DATMXJ010000000
VERSION     DATMXJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34025"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VN377_03925"
     CDS             <1..295
                     /locus_tag="VN377_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27570.1"
                     /translation="NKIINNLLIKFIYMSNGHSDLDKYFPAGSAELNSVRQREENLIQ
                     SGTFTDTTPLVTKREFNTAITKMEKDANRKFLYGIAIGIIGILVTIITAILLC"
     gene            complement(575..2431)
                     /locus_tag="VN377_03930"
     CDS             complement(575..2431)
                     /locus_tag="VN377_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27571.1"
                     /translation="MLVLSCVVPCESTIDETEVGSEEEYREYMNDWFVHTDDSNGIIG
                     SAYSDIQNKGSSDDMIVKKTSSSNGGLQNSSWPMSCHDTYHTSQSSISTADNPGTEKW
                     RYKTLQDAGTVESGAIVDNNGIIYFGSMGADHKLYALYPNGTMKWYYKTPAAGIIWST
                     PTIADDGTVYVGDWNGRLSALDQNGSLLWQLYRSGEFASSPAIGDDGTIYLGHSGGCL
                     YAINPDGSEKWHYETGYYVNSGPAVGMDGTIYIGSADNYIYALYPNGVIRWRYLTGGV
                     IKGHPSIAPDNKIYVPSFDGNLYALNPNGTLIWKVSTGDEVAAASAAIATDGTLYVGT
                     EVLRAFYPNGTLKWMVDVGGDVYGTSPAVSADGTIYVSAGVCIVAVNPDGTIKWKKLI
                     SNVAVLSSPCISEDGSIYVGSSWRNHDGTLTWGYLHAFGEAPLSVDAGGPYSGNAEQS
                     ISFMSTTFGGALPYTFQWEFGDGNTSNLEHPTHTYSRPGEYIATLTLVDGEGNSSSDT
                     ANVTVGTSLPTVRILRPENALYLFNKKIMPLKRPVIIGRITIKVEAFQEDVGIDHVSF
                     YIDSGYKYSDYEAPYEWDWTDRSFSFYDIGVAAESKDGKWGGADIYNVLKIF"
BASE COUNT          776 a          623 c          517 g          755 t
ORIGIN      
        1 gaataaaatt attaataact tattgattaa atttatttat atgtcaaatg gacactcaga
       61 tttggataaa tattttccag ctggttcagc agaattaaac tcagtaaggc agagagaaga
      121 aaatctaata caaagtggta cattcacaga taccactcca ttggttacaa agagagaatt
      181 taatactgct ataactaaaa tggaaaagga tgcaaataga aaatttttgt atggcatagc
      241 tattgggata attggaatat tagtgacaat aataactgct atactcttgt gttaattcag
      301 ataccaacgc taatttcgac tctgctgaat tgaggagtgt tggaattgta gtgttgaatt
      361 aggtgtggca gttttccccg ttatcatatt ttataacact gttcgcctat aaaagaatat
      421 ccttggaagt ataaaataaa agggtctgaa cggaaggtct cgcatataca atttcatacg
      481 gtctcttatc gtcttaattc ctaacaaaca taggtacacg acattgatag attcactcga
      541 actgttgctg gtgttagaaa atgttaatag attcttagaa aatctttaac acattataga
      601 tatcagcgcc gccccatttt ccatcttttg attctgccgc aacaccaatg tcatagaatg
      661 agaaacttct atccgtccaa tcccactcgt agggtgcctc ataatcagaa tacttgtaac
      721 ctgaatcaat ataaaatgag acatggtcga ttccgacgtc ctcttgaaac gcttcaactt
      781 taattgttat tctcccgatg atcaccggac gtttcagagg cattattttt ttgttgaaca
      841 agtacaaagc attttccggg cgtaagattc tcactgttgg tagagatgtc cctaccgtca
      901 catttgcggt gtcgctactg ctatttccct caccatcgac caatgtaagc gtcgcgatgt
      961 actcaccggg tctggaatag gtatgtgtcg gatgctccag gtttgaggtg ttcccatcac
     1021 cgaactccca ctggaacgta taggggagcg ctcctccaaa ggtcgtactc atgaatgata
     1081 tactctgttc agcgttcccg ctgtagggtc ctcctgcatc taccgataat ggtgcttcgc
     1141 cgaacgcatg gagatacccc catgtaagag taccatcatg attcctccaa gatgagccga
     1201 catagattga accatcctcg ctgatgcagg gtgatgatag cactgcgaca ttcgagatta
     1261 acttcttcca cttaatcgtc ccgtcagggt tcaccgcgac aatacagaca ccagcgctca
     1321 cataaatcgt tccatccgcg gagaccgctg gtgacgtccc atagacgtcc ccgccgacgt
     1381 caaccatcca tttcaaggtt ccattcgggt aaaacgcccg taagacctca gtccccacat
     1441 acaacgttcc atcagttgcg atcgctgctg atgcagctgc gacttcgtca ccagtactta
     1501 ccttccaaat taatgtccca ttgggattta gagcatacag attcccatcg aacgagggaa
     1561 catagatttt attatctggt gcgattgaag gatgcccttt gatgacaccg ccggtaagat
     1621 accgccagcg aattacgcca ttaggataga gagcataaat ataattatca gcagaaccaa
     1681 tgtatatggt accgtccata ccaacagcag ggcctgagtt tacgtagtat cctgtctcat
     1741 aatgccattt ttcgctccca tctggattta ttgcatagag acaaccacca gaatgaccta
     1801 aataaattgt cccgtcatca cctatcgcgg gggaagatgc aaactcccca ctacgatata
     1861 gctgccatag taatgaaccg ttctgatcaa gagcagagag acgcccattc cagtcaccaa
     1921 catagacggt cccatcatca gcaatagttg gcgtagacca tattattcca gctgcaggtg
     1981 ttttatagta ccatttcatc gtaccattgg gatacaatgc gtagagttta tgatctgccc
     2041 ccatgctccc aaaatagatg atcccgttgt tgtcgacaat ggcacctgat tcgacagttc
     2101 ctgcatcttg caatgtttta tatcgccatt tctcagttcc tgggttgtcc gcggtgctga
     2161 tagagctttg actggtgtga tacgtatcat ggcagctcat cggccatgaa gaattttgca
     2221 aaccaccgtt agaactcgat gttttcttta cgatcatatc atcgcttgat cctttatttt
     2281 gaatatccga ataagcagat cctatgatcc catttgaatc atcagtatgt acaaaccaat
     2341 cattcatgta ctctcgatac tcttcttcgg aaccaacttc agtttcatca atagtcgatt
     2401 cacaaggaac cacacaacta agaacaagca caactaacaa tccaaccgca agagctttcg
     2461 gtatcagatg atttcccccg ttctacaagt ttataacaat gttctccttt aaaaacatat
     2521 cctgagaaat ggcttcatcc cgaaatagat aactaggaga taaagctcta tttttcgttg
     2581 gatgggatgc gcaaaacaac ggatgaaccc aaccgttgcg aaccatgtta accgcaattt
     2641 caccgctctg ttcactcggg cggtggtaaa a
//
LOCUS       DATMXJ010000145         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_7972, whole genome shotgun sequence.
ACCESSION   DATMXJ010000145 DATMXJ010000000
VERSION     DATMXJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7972"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..>2112
                     /locus_tag="VN377_03935"
     CDS             488..>2112
                     /locus_tag="VN377_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27572.1"
                     /translation="MNISLNATSSSHWVAQNISNGYFPWGIGWARYGFVPRLDVVGNI
                     TFNGTSSQSRGIAYYEHLWGNFSYLITMKSPSEFIAFVKNLPKVMPFMKWIWTEQSRT
                     SSQLKLSTSNHYGYDWIWGSFTNGWSVHLGFFHTMGGEGSLTPDGQTFWDFANFSVKY
                     TRLLYIPETDTYYPLDIDIIAKSGDKTLHLSLTTTTEPYLGLLVIYPASRLCCGAGGI
                     QTAGIVEGYYTDSSQNISLQGNCTIGPFRQYPTTRFESLEMVRIQSPQATGFSIEIVS
                     HLVGARLYIERLRRPHHQLNISLEISRPPSPVKPPEQPYDGTTLYVGGDGPGNHTHIQ
                     DAVNLASDGDTVFVHGGDYHENVVVDKAIRLVGENKGAVFLTGGTRAAIKIIANNVEI
                     TGFTIDGTQANSHDDAAIYVASSGNHIYDNLLIKSEWYGIIIFNSSYNVIENNSIIDN
                     DIGIWLCQARDNTVRNNIISQSNYVGIWLWPFAIHNTISYNTFVDNKINARNNDATAR
                     NIWSYNYWDDYIGLRWRDVVDPNNHGTGIIPYK"
BASE COUNT          626 a          461 c          428 g          597 t
ORIGIN      
        1 acattcacaa tctgataata atttcttaaa aaacaattcg agatggatta cgattatcat
       61 cctttttgta ttattgttaa ctgccctttt accctgtact gcgtctgcaa ctgaaaaaaa
      121 caatcaatta catttcaccc ctgatgaaga gggagatcac tttccttgtg gctgcgaatg
      181 gtggtgcttc catagctcag taacaacaca aactggaacg cacctagaca tacttaccga
      241 attccaatat tcgacaaaag aagtaaacag aagtgacatc aatatggtcc tttttctctt
      301 ttattgcttt aacagagata ctggacaggt ctttgacttc acacagtcct ttgtgaacaa
      361 aggcaatcaa tcgggtccat tctcgttcaa aaaaaattac ctagacattc gttttggcaa
      421 ttgtacaata aaaggacttt atccatcgta tttggtgtat gttgagaatg aggacaaaag
      481 cttcatcatg aatattagtt tgaatgccac ctcatcgtct cactgggtcg ctcaaaatat
      541 aagtaacggg tattttcctt ggggaatagg atgggctcga tatgggttcg tccctcgact
      601 cgatgtcgta ggcaacatca cgttcaatgg gacatcatct caatcgaggg gaatcgcata
      661 ttatgagcat ttgtggggaa acttttctta tttaataaca atgaaatcac cttcagagtt
      721 catagcattt gtgaagaatc tacccaaagt catgcctttt atgaaatgga tctggactga
      781 acagtcgaga acttccagtc aactgaagct tagtacatcc aatcattacg gctatgattg
      841 gatctggggt tcgtttacaa atggatggag cgtgcacctt ggctttttcc atacgatggg
      901 cggggaaggc tcgctgacac cagatggaca gacgttttgg gattttgcca atttcagtgt
      961 aaaatataca aggcttctct acattcccga aacagatacg tattatcctc ttgatattga
     1021 cataatcgcc aagagcggag acaaaacatt acatctttct ctaacgacta ccactgagcc
     1081 gtatcttggc ctgcttgtta tttatccggc ttcgcgctta tgctgcggtg caggaggtat
     1141 ccaaacagca ggtattgtag aaggttatta cactgattcc agtcaaaata tttctcttca
     1201 gggtaattgt actatcggtc ccttcaggca atatccaacg acacgattcg aatctctaga
     1261 aatggttcgg attcaatccc cgcaggctac tggattcagc attgaaattg tctcacattt
     1321 ggtgggcgca agattatata ttgagcgact ccgacgccct caccaccagt tgaatatttc
     1381 tcttgagata agtcgaccac catccccagt taaaccccca gaacaaccct atgacggcac
     1441 caccctctat gtcggcgggg acggccctgg gaatcatacc catatccaag acgcagtcaa
     1501 tcttgcctct gatggtgaca cagtgtttgt tcatggaggg gattatcacg aaaacgttgt
     1561 agtcgacaaa gctatccggc ttgttggaga aaataaaggt gctgtcttcc ttacgggtgg
     1621 aacgagggct gccatcaaaa taattgcgaa caatgtggaa atcactgggt tcacaatcga
     1681 tggaacacaa gcgaacagcc atgatgatgc agcaatttat gttgcctcct ctgggaatca
     1741 tatctatgat aacctgctta tcaaatcaga atggtatggg atcatcatct tcaattcctc
     1801 ctacaacgtc attgaaaaca acagtatcat cgacaatgat ataggaatct ggctgtgcca
     1861 ggcacgagac aacaccgtac gaaacaatat tatctcacaa agcaactatg taggaatttg
     1921 gctctggcct ttcgctatac acaatacaat ctcttataat acgtttgttg acaataaaat
     1981 caacgcaaga aataatgatg caactgcgcg gaacatctgg tcttataatt attgggatga
     2041 ttacatcggc ctacgttgga gagatgttgt tgatcctaac aatcacggca ccggtatcat
     2101 tccatacaaa at
//
LOCUS       DATMXJ010000146         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_8029, whole genome shotgun sequence.
ACCESSION   DATMXJ010000146 DATMXJ010000000
VERSION     DATMXJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8029"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..2301
                     /locus_tag="VN377_03940"
     CDS             256..2301
                     /locus_tag="VN377_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR27573.1"
                     /translation="MHRGLWIKGLAVGIIILFVGMGILPSVAQNIEKPSIPVMSGNWL
                     YVGGFGPGNYTHIQDAINTASDGDTIFVYDDSSPYYEHLLIDKSITLLGQNQSSTIID
                     GANTSTVGHITSDCVTIRQFTFRNSGGEQTDAGIRITANATTLIDCTVYRARTGVIIQ
                     RGTNHIIMDCDVHTTGEGIYCTQTNALVISHCQFCHNAIGLRLNASINSTIDNTFFHT
                     NGLGCYFDHASDITMDHCALDDNNDNQGGAYLTSCTDILFNNSNICHNGVGINIGNSH
                     DIQIAYCDMNRNTKLAVLVDGATSNISLIGNEISHNYHYGIYTAAPLIAHDNNFYQNE
                     LHALYATIPCDAPYNWWDTFFGPAFTELGPGERISGNMVHHFPWLLHASSDAGSTWET
                     ATSFVPLRIPLNARPLIIVGNDTDQDCAPDWWELKYNYDPYHWDNHAILDPDRDGLTN
                     VEECYTDQWGSHPFKRDIFLEIDWMESNVTGTTNKPDQTLIEQAIALFETHNITLHID
                     TGAMGGGEMIPYHASPDYLYFRDLYWTYFLHQDLNSPRKGIFHYAIITDDGEAGYTIF
                     GWDQLDFFNVGAQQTQEKYPWYTKKHIIIHVVLHELGHDLGLFGDTFGGIDNRESTKF
                     TSWQTWKYLPYRSCMNYHYTYRILGYSDGTHGPGDFDDWAHLNLSLFKNTSFPVLSS"
     gene            2484..3182
                     /locus_tag="VN377_03945"
     CDS             2484..3182
                     /locus_tag="VN377_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWR27574.1"
                     /translation="MAENEKSPEEKKQHPTKEPAETKPSEGKETPERTDAVTPGPGAA
                     KPDQQKKKEKKGKQTAKEDEPVKKKEHDENFNYIIRVVNTDINGESNIVQGLTQIKGI
                     GRHMATFIADTAGVDRKLKFGNLPESEIEKLKEVLENIDEYAPPWMLNYRKDAYSGED
                     IHLISTDVTSRLRDDINMMKMIRSYRGVRHELGLKVRGQRSSSNGRKGLALGVSKRRE
                     APAAAGKKEESKEK"
     gene            3194..>3483
                     /gene="rpsD"
                     /locus_tag="VN377_03950"
     CDS             3194..>3483
                     /gene="rpsD"
                     /locus_tag="VN377_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWR27575.1"
                     /translation="MSSQKFPRKRYETPMHPWKESRIKTERELMKKYGLKNHKEVWKA
                     KTYLGKHREQARELLAKVGTASPQAKKESDQLLLHLSRMGIVAMGASLDDVLA"
BASE COUNT         1073 a          901 c          725 g          784 t
ORIGIN      
        1 ctgtcaatat gtcctcaatc gatgtccagt ctgttttctg catacatgcc caactcacta
       61 aggaggatat atttgctgca tttatcaatt acggtaatct cttcgaccag atactctctc
      121 taaaacaagt atcactttca ctccccttca ataatcccaa ttgaagaaaa cccttttttt
      181 tatatgtttc acaacattta aatgaatcca tagaataaca aacccaacct gttcatcaag
      241 ggagaatcta ggagtatgca tcgtggatta tggatcaaag gattggcagt tggaatcatc
      301 attctgttcg ttgggatggg catcctcccc tcagttgctc aaaatataga gaaaccatcc
      361 ataccagtga tgagtgggaa ttggttgtat gttggtggtt ttggtcctgg gaactacacc
      421 catattcaag atgcgatcaa caccgcatca gacggggata ccatatttgt ctatgacgac
      481 tcctccccct actatgagca tctgttgatt gataaatcca tcacgctcct tggccaaaat
      541 caatcatcaa caatcatcga cggcgcgaac acaagcacag taggtcacat cacgagtgat
      601 tgtgtcacca tcagacagtt caccttccgc aactctggtg gtgagcagac cgatgctggc
      661 atccgtatca cagcgaatgc taccactcta atcgactgca ccgtgtaccg tgccagaacc
      721 ggtgtaatca ttcagcgggg aaccaatcat atcatcatgg actgtgatgt tcacaccacg
      781 ggcgaaggaa tctattgtac tcaaaccaat gctcttgtta tttctcactg tcaattctgt
      841 cataatgcca tcggcctacg actgaatgcc tccatcaaca gcaccatcga taacacgttt
      901 ttccatacca acggcctcgg ttgttatttc gaccacgcat ccgatatcac aatggatcat
      961 tgcgcccttg acgacaacaa cgacaatcaa ggcggcgcgt acctcaccag ctgcacggat
     1021 atcttgttca acaacagcaa catctgccat aacggagtcg gtatcaatat cggtaactcc
     1081 catgatatcc aaatagcata ttgtgacatg aaccgtaaca ccaaacttgc tgtccttgtc
     1141 gatggcgcca cgagcaacat ctccctcatc ggaaatgaaa tcagccacaa ctatcactat
     1201 ggtatctata ctgctgctcc tctcatcgcg catgacaata atttttatca aaatgaacta
     1261 cacgctctct acgcaaccat tccctgcgat gctccataca actggtggga cacttttttt
     1321 ggtccagctt tcactgagct gggtcccgga gaacgcatca gtggaaacat ggtccaccat
     1381 ttcccctggc tcctccacgc gtcctcagac gcgggatcca cctgggagac cgccacctcc
     1441 tttgtccccc ttcgcatccc cctaaacgca cgtccgctca ttatagtagg gaatgacacc
     1501 gatcaggatt gtgcaccaga ctggtgggaa ctcaaataca attacgaccc ctaccactgg
     1561 gacaaccacg ctatcttaga cccggaccgt gacggcctca ccaacgtgga ggaatgttac
     1621 acggaccaat ggggatcaca cccgtttaaa cgagatattt tccttgagat tgactggatg
     1681 gagtccaacg tcaccggaac aaccaacaaa cccgaccaaa cactgataga acaagccatc
     1741 gccctttttg aaacccacaa tatcaccctt catatcgaca ccggagccat gggcggagga
     1801 gaaatgatac cctaccacgc ctcacccgac tatctctatt ttcgcgacct ctactggacc
     1861 tatttcctac atcaggacct caactcacca cgaaaaggga ttttccacta cgcaatcatc
     1921 actgacgacg gcgaggcagg gtacacaata ttcggatggg atcagcttga tttctttaac
     1981 gtcggcgcac agcaaacaca ggaaaagtac ccctggtaca caaaaaaaca catcatcatc
     2041 cacgtcgtcc tgcatgagct aggccatgat ctaggattat ttggcgacac gttcggaggg
     2101 atcgataacc gtgagtccac caaatttact tcatggcaaa cctggaaata ccttccttat
     2161 agaagctgca tgaactacca ttacacctac cggatactcg gatactccga cggcacgcat
     2221 ggaccaggag attttgatga ctgggctcat ctgaacctct ctttattcaa aaacaccagt
     2281 tttccagtac tctcttcctg ataaaaacat aattttttgg tcaaagaacc tcgatttgac
     2341 cattggtatc attctcctta ccttcggaac aaccgcctca agggttaccc ttaaataggg
     2401 cctcttttat tattggcaat actttcttgt agtattcaaa gtactataga gagagcgctt
     2461 gtacattaaa acggggtatc atcttggcag aaaatgaaaa atcaccagaa gaaaagaaac
     2521 aacatcctac aaaggaacca gcagaaacaa aaccatcaga gggaaaagaa acccctgaac
     2581 gaaccgacgc tgtgacacca ggacccggag cagcaaaacc agatcaacag aagaaaaaag
     2641 agaagaaagg aaaacaaact gccaaagaag acgaaccggt gaagaaaaaa gaacatgatg
     2701 agaattttaa ctatatcatc cgtgtggtca acaccgatat caacggagaa agcaacatcg
     2761 tccagggtct gacccagatc aaaggaatcg gacgacacat ggccacattc atcgctgaca
     2821 ccgccggggt tgaccggaaa ctaaaattcg ggaacctccc ggaatccgag attgaaaaac
     2881 tcaaagaagt cttagagaac atcgatgaat atgcgccccc gtggatgctg aactaccgga
     2941 aagatgcgta cagtggtgaa gacatacatc tgatcagcac tgatgtgacg tcacgccttc
     3001 gggatgacat taacatgatg aagatgatac gatcctaccg cggagtacgc cacgaacttg
     3061 gtcttaaagt acggggacaa cgaagcagtt cgaatggcag gaaaggctta gcattgggtg
     3121 tctcaaaacg aagagaagca ccagcagcag ctgggaagaa agaggagtct aaggagaagt
     3181 aggagtgatc tagatgagca gtcagaagtt cccaagaaaa agatatgaaa cccccatgca
     3241 tccctggaaa gagagccgta ttaaaactga acgagaactg atgaaaaaat acgggttgaa
     3301 aaaccataag gaagtctgga aggcaaagac ctacctcggg aaacatcgag aacaagcaag
     3361 agagctgctt gcaaaggtcg gaaccgctag cccacaggcg aaaaaagaaa gcgaccaatt
     3421 actcctgcat ttatcccgga tgggtatcgt agccatgggg gcatcacttg atgatgttct
     3481 cgc
//
LOCUS       DATMXJ010000147         6742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_49891, whole genome shotgun sequence.
ACCESSION   DATMXJ010000147 DATMXJ010000000
VERSION     DATMXJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49891"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VN377_03955"
     CDS             <1..981
                     /locus_tag="VN377_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27576.1"
                     /translation="KAGLVMCIAALFIGTSAAAIGSTPEKLILTSTPKTATLYSPTRE
                     EVELKYYMEEGLSQVIGIGGGTGPYVWKTAIRLTQDEMAAYTDWTLTKVNVAFSMDNG
                     LTNPMDIKIYIYDKGTTSTRPGAVIVSDTTASLDTTGITTVPLVTPVDLSGHEELWVA
                     IEWTQIENDNVWYAWLDTVTGPHVPQKGDFIYLNNAWSEIYTGGVAYDGNWGIGAIVE
                     GAGLAELSIGNIKGPMGIKADVSNIGANDATNVQWSIAVTGGLLKRVNVSNPGTVAAL
                     AAGTSTPISLPAFIGFGKISIVITAKAQNANEVSATKSAFLLGLFVIGIK"
     gene            complement(1084..1371)
                     /locus_tag="VN377_03960"
     CDS             complement(1084..1371)
                     /locus_tag="VN377_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27577.1"
                     /translation="MKLESLDEMEENKRFFCWSCGKQLQAVDEISQRICFQCKQTIKD
                     LKEDDAFFCWACGKQLLHMSEVAQGVCHPCKASIIRKIHSPAKNTTPPLKN"
     gene            complement(1454..2407)
                     /gene="mtnA"
                     /locus_tag="VN377_03965"
     CDS             complement(1454..2407)
                     /gene="mtnA"
                     /locus_tag="VN377_03965"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWR27578.1"
                     /translation="MKIDGRDYRAVWQEGQFVTFIDQRKLPYSFEFFTARTVDDVAYA
                     IKDMVVRGAPAIGVAAAYGMALGQRNPKRSADLLKSTRPTAYDLFYAIDSMTQAIERN
                     ENAFDAAATYAETVITRCKKIGENGEKIIKDPMRILTHCNAGALATIDYGTALAPLRK
                     AHEKQKKIFVYADETRPRLQGLLTAWELFHEGIPYALITDNAAGYFMRKKDVDLVIVG
                     ADRIAKNGDFANKIGTYEKAVVAKENNIPFYVAAPVSTFDPQLRDGSQIIIEERGRQE
                     ITEISGKTIMPEWVSVKNPAFDVTPKKYVTGYITEDGIRKI"
     gene            complement(2404..2970)
                     /locus_tag="VN377_03970"
     CDS             complement(2404..2970)
                     /locus_tag="VN377_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWR27579.1"
                     /translation="MIDRILPAPALQTLFISRETSKCPLLSDMITLGQSLQDFGLTDK
                     DTGILSMDYGKRLLINAKNVDLKKITQQNIVEIVDYDPLKNIMMVIGLNDPALETPVH
                     WIIQKARHDVNAVLQINSLTLFERFKNTFPTVEKNTQPGTLERAKELLRILRNGKTVL
                     IQNEGIVFVGINGKEIHDALIRHLEEQK"
     gene            3504..4175
                     /locus_tag="VN377_03975"
     CDS             3504..4175
                     /locus_tag="VN377_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWR27580.1"
                     /translation="MLKLDMHVHSCFSNDGVGTPQDLIKSLQKQGLQGMALTDHNTID
                     GYRTVQHMVPSDFLVIPSVEISTADGHLLAINVTENIQAGLSVEETVEQVIDAGGEPI
                     VPHLFRLFSGIKKEKLKTIQTKVSAIEVFNGCSVPNTNLKTAKVAHAFNLGGTGGSDS
                     HDPTFAGYAYTVVDSTDLQIDSVLSEIKKKKTWGEGVTMPLTYRRDRMVLSIRQYVSR
                     GFKRI"
     gene            4372..>6742
                     /locus_tag="VN377_03980"
     CDS             4372..>6742
                     /locus_tag="VN377_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27581.1"
                     /translation="MKKHTNSKRANWKQVFIVSIAFLMICSVAAQSKQATVDPNVKRT
                     YSFNSPSFVTIDIAGTIYDRVTLDDCYPAGSAGEPKIPSKGVFLLLPPDSQVSGIEIT
                     TGEKIILGSGFNVEPTSQAIPISQTDNIPIPTPNPAIYQSNAAYPGKLFTQVGVQYFR
                     GYQILVLLLHPVQYNPVTGELFYYTSLEVSVQTTPSGVQSALYRGLNQDIQQVQQKVD
                     NPEMTLAYDQQYTSSPASFEDYDLLIITTDALKSGFEALAEAHTATGVATVIKTLTDI
                     GGSDLESIRNYIRDAYTNWGVEYVLIGGDSDVIPAPILWVSGMDENVTYYEDTMPADL
                     YYACLDGPYNYDNDGYWGEPHDGTDGGDVDLIAEVYVGRACVGSTTEVNNFVTKTVAY
                     INRDVNDPYLKKICLAAEYLGDYGIASYGGTYMDQLVNGSTDDGYTTVGISSDDYVIN
                     KLYDSPGYDWPPSAIISVINNGVHAINHLGHANEVYNMKLDTSDVDGLTNPSNRTCFI
                     YSQGCIAGAFDYSDCIAEHFTIKTTHAAFAGIWNARYGFFWSYSTDGDSQRLHRQFWD
                     AVFGEMIPEIGRANHDSKEDNLPIIGRSCIRWCYYETNLFGDPALKITEITNNPPATP
                     SLPSGPTSGKIGIDYTFSSSTTDPDTGAQLFYQWSWGNEESGWLGPYESGQSIQAIHQ
                     WSTAGDYQVKVRAKDNLGSMSAWSDSITIQIVEGPQIEIGTISGGMKITAEIKNTGIV
                     DASHVNWSITLQGLVLFGQEKSGTLLGISPGDSLDVSTGLILGLGTVDIT"
BASE COUNT         1859 a         1434 c         1466 g         1983 t
ORIGIN      
        1 aaggctggtc tagtcatgtg tatcgcagca ttgtttattg gaacatctgc agctgcgatc
       61 ggaagcacac cagaaaaact aatcctgacc tcaacaccaa aaaccgcgac tctgtatagc
      121 ccaacccgtg aagaagtcga attaaaatat tacatggagg agggtctttc ccaggttatt
      181 ggtataggtg gaggtactgg cccttacgtc tggaaaaccg ctattcgact gacccaggac
      241 gaaatggcag cctacacgga ttggaccctg acaaaggtca atgtggcatt ttcaatggat
      301 aatggtctca ctaacccaat ggatattaaa atttatattt atgataaagg aacaacttca
      361 acaagacccg gagctgtgat tgtcagtgat acaaccgctt cattagacac cacgggtatc
      421 actacggttc ctctcgtcac ccctgttgat ctcagtggtc atgaagaact ttgggttgca
      481 atcgaatgga cacaaataga aaatgataat gtatggtatg cctggttaga cactgtaact
      541 ggtccccatg tccctcagaa aggtgatttt atttacttaa acaacgcatg gagcgaaatc
      601 tacactggtg gagttgcata cgacggaaac tggggaatcg gtgctatcgt cgaaggcgct
      661 ggcctagcgg aattatcgat tggaaacata aaaggaccaa tggggataaa agctgatgtg
      721 tcaaacatag gtgctaatga tgcaactaat gtccaatggt caattgctgt caccggtggt
      781 ctgttgaaac gtgtaaacgt atctaacccg ggaactgtag cggcacttgc agctggaaca
      841 tcaactccga taagtcttcc agcatttatt ggatttggta aaatcagcat cgtgattacc
      901 gcaaaggcac aaaacgccaa tgaagtatca gcaactaagt ccgctttctt acttggactt
      961 tttgttattg gaattaagta aaaccactta attctctctt ttttttcttt ttcttataaa
     1021 aaatattaat tgttttattg catccttttc gcgttttaaa tagaaagaaa tcctattgtg
     1081 atgttaattt tttagtgggg gcgtagtgtt ttttgcagga gaatggatct tacgaatgat
     1141 tgatgctttg cagggatggc agactccttg ggcgacttca ctcatgtgca acagttgttt
     1201 tccgcacgcc cagcagaaga aagcgtcatc ttcttttagg tcctttattg tctgtttaca
     1261 ttgaaaacag atgcgctgtg atatctcatc aacagcttgg agttgtttgc cgcaggacca
     1321 acagaaaaat cgcttatttt cctccatttc gtctaaactt tccaacttca taggcatcca
     1381 ttccgaacca attcttctat ccttcttttt cgaatcgtgt ttcgtatata aacgattttc
     1441 ttatctttcc tttttaaatt ttacggatcc catcttctgt gatatacccg gtaacgtact
     1501 tctttggggt gacatcgaat gctggattct tcaccgacac ccactctggc atgatggttt
     1561 tcccagagat ctcggtgatc tcttgtctcc ctcgttcttc gatgatgatc tgtgagccgt
     1621 ctctgagttg tggatcaaaa gtacttactg gtgcagcgac ataaaacggg atgttgtttt
     1681 ctttggcgac tacggctttc tcatatgttc ctatcttgtt tgcaaaatca ccgttcttcg
     1741 cgatcctgtc tgcaccgaca atcaccagat cgacgtcttt ttttctcatg aaatagcctg
     1801 ctgcgttatc cgtgatcaat gcatagggga ttccttcgtg gaaaagctcc caggcggtca
     1861 gcaagccttg aagtcgtggt cgtgtttcat cagcgtagac gaatatcttt ttttgttttt
     1921 catgtgcctt tcgtaagggt gctaacgcgg tcccatagtc tatggtcgct aatgctccag
     1981 cgttacaatg ggtcaagatc ctcatcggat ctttgattat tttctccccg ttttccccta
     2041 ttttcttaca gcgggttatg actgtctctg cgtaggttgc tgctgcgtca aaggcatttt
     2101 cattacgttc tatcgcttgt gtcatagagt caattgcgta gaagaggtca tacgcggtcg
     2161 gtcgtgtcga tttgagcaaa tctgcggatc tcttagggtt tcgttgtcct aatgccatcc
     2221 catacgcggc tgctacacca attgcaggtg cccctcggac caccatgtct ttgattgcat
     2281 aagcgacatc atcgacggtt ctggctgtaa agaattcaaa agagtatggg agttttcgct
     2341 ggtcgataaa tgtaacaaat tgtccttcct gccatactgc tcgatagtct cttccatcga
     2401 ttttcatttt tgttcctcaa ggtgtcgaat gagtgcatca tgaatttctt ttccattaat
     2461 gcctacgaaa acgatccctt cgttttgtat caacactgtt tttccatttc gtaaaatccg
     2521 taagagttcc ttggcacgct cgagggtccc tggttgagtg tttttctcta ctgttgggaa
     2581 tgtgttctta aatctctcaa aaagagtcaa gctgttgatc tggagtactg cattgacatc
     2641 atgacgtgct ttttggatga tccaatgtac cggggtttcc aatgctgggt cgtttaaccc
     2701 gatcaccatc atgatgtttt tcaagggatc gtagtcaacg atttctacga tgttctgttg
     2761 cgttattttt ttaagatcaa cgttttttgc attgatcaga aggcgtttgc cgtaatccat
     2821 gctcaggatc cctgtgtctt tatcagtcaa cccaaagtct tggaggctct ggccgagggt
     2881 tatcatgtca gatagtaagg gacacttgct tgtttccctt gagatgaaaa gtgtttggag
     2941 tgcgggagct ggtagaattc tgtcaatcat aacaggatgg taacaaggat gggatttttt
     3001 atgttttgcg ttggagctgc tttaaaggaa tcaaatctgt gcgtttaatc tcattatcac
     3061 ctggttttaa tagaagttac gcatcgccgg tttccgacga accatcgaag gatcgagatc
     3121 aaaaataagg acatcatcaa agaggaattc aaataccgag tcacgcgaaa gattctatta
     3181 atccttccga gatttccacg attaaatacg ttttttaaag attatggaaa agatagagga
     3241 gaaatggtta gttatctcgt ttgttcgtgc aaagaaaaaa caaggattta tctacgagtt
     3301 ctaactcacc aaatctatgt gtacttaact gtttgttttt ttcaaatagc ataattctgc
     3361 ctctcccaaa ccgctacaat agtcttgggt tatttaacgc tttcttttta tttcttcctc
     3421 ctggagtatc cgagggcgag ggcgtcttac cgtaaatttc taataggaat ttaagcttcc
     3481 atatagcagc ggaggtttct cctatgttaa aattagatat gcatgttcac tcctgttttt
     3541 ccaatgatgg ggttgggact ccacaggatc taatcaaatc attacaaaaa caaggtcttc
     3601 aaggtatggc actgacagat cataacacca tcgatggata tcggacggtg caacatatgg
     3661 taccaagtga ttttcttgtc atcccgagcg ttgagatatc aaccgcagac gggcaccttc
     3721 tggcgatcaa tgtgactgag aatatccaag ccggtctttc tgttgaagaa acagtcgagc
     3781 aagtcattga tgctggtgga gaacctatcg tcccgcatct ttttcgtctt ttttccggga
     3841 ttaaaaaaga aaaattaaag acgatacaga ccaaagtctc tgctatagag gtttttaatg
     3901 gctgtagcgt cccgaatacg aacctgaaaa ccgccaaagt tgcccatgca ttcaatcttg
     3961 gtggtacggg gggaagcgat tctcatgatc ctacctttgc aggatacgca tataccgttg
     4021 ttgattcgac agatctacag atagattcgg ttctctctga aattaaaaag aaaaaaacct
     4081 ggggcgaagg agtcaccatg ccacttacct accgacgaga tcgcatggtc ctttcgatcc
     4141 gacagtatgt gtcacgagga ttcaaacgaa tctaagagat ttttccaata ccattaacta
     4201 ttccataaat cggatcagta ttgtatagat ataaaaacca aagggattta ctactatcat
     4261 caacaggagg aaatgaatat cttggtcgat gcaggaaatc tagaaattgt gtgcaacatc
     4321 catgtttata tataggaacg atgtatatca tgtactgata ggggataata tatgaagaaa
     4381 cataccaatt caaaacgagc taattggaag caggttttta tcgtctcgat agcatttcta
     4441 atgatatgtt ctgtcgcagc tcaatcaaaa caagctactg tcgacccgaa tgtcaaaaga
     4501 acgtattctt ttaacagccc atcttttgta actattgaca tagcaggaac gatctatgac
     4561 cgggttacct tagatgactg ttaccctgct ggaagtgcgg gtgaaccaaa aataccttcg
     4621 aaaggggtat tcctcctctt accacccgat tcacaagtca gcggaattga aataacaacg
     4681 ggggagaaaa tcattcttgg ttccggattt aatgtagaac caaccagtca agcgattccg
     4741 atatcacaaa cagacaatat accgattcca actcccaacc cggctatcta tcagtcaaat
     4801 gccgcatacc ctgggaaact gttcactcag gtcggcgtcc aatatttccg aggatatcaa
     4861 attcttgtgc tgcttttaca tcctgttcaa tataaccctg taaccgggga gcttttctac
     4921 tacaccagct tggaagtctc ggtacagacc accccatctg gtgtccaatc agctttatat
     4981 cgaggtctaa accaagatat acaacaagta caacagaaag tagataatcc agagatgaca
     5041 ctagcctatg accaacagta tacttcttca ccagcatcat ttgaagacta cgatcttctt
     5101 attatcacaa ccgatgcact aaaaagtggt tttgaggcac tagcagaagc acatactgcc
     5161 accggtgtcg caactgtgat aaaaacactg acggatatcg gcggaagcga tctggaaagt
     5221 atccgaaact atatccgtga tgcctatacc aactggggag tggaatatgt cctcattggt
     5281 ggagatagcg atgtcatacc cgcaccgatt ctatgggtgt cagggatgga tgaaaatgtt
     5341 acctattacg aagacaccat gcctgctgat ctctactatg cctgtcttga tggcccctat
     5401 aactatgata atgatgggta ctggggtgaa ccacatgatg ggactgatgg aggcgatgtc
     5461 gacctgatag cagaggttta tgtcggaaga gcatgtgtcg gatccacaac cgaagtaaat
     5521 aatttcgtta caaaaacagt cgcttacatc aatagggacg tcaatgatcc ttatctgaaa
     5581 aaaatatgct tagcagcgga ataccttggg gattacggta tcgctagtta tggcggaaca
     5641 tatatggatc aactcgtcaa cggctcaact gatgatggat ataccaccgt tggtatatct
     5701 tcagatgatt acgttataaa taaattatat gactctccgg gctatgactg gccaccatca
     5761 gcgatcattt ctgttatcaa caacggcgtc catgccatta atcatcttgg ccacgcaaat
     5821 gaggtttata atatgaaatt agatacctct gatgttgatg gactgacgaa cccctcaaat
     5881 cgaacctgct ttatctattc tcagggatgc atcgctggtg cttttgacta tagtgactgt
     5941 atcgcggaac atttcacgat aaaaacaacg catgctgcat ttgctggtat ctggaacgca
     6001 cggtatggat ttttctggtc ttatagcacc gatggagatt cacaacgcct ccatcgtcag
     6061 ttctgggatg cggtgttcgg tgaaatgata ccggagatcg gacgtgcgaa tcatgattca
     6121 aaagaagaca atcttcctat cattggacga tcgtgcatac gctggtgtta ctatgaaaca
     6181 aacctgttcg gtgatcctgc attaaaaata actgaaataa ccaacaatcc cccagccaca
     6241 ccttcattac catctggtcc gacatctggg aagatcggta tcgactacac gtttagctca
     6301 agtacgactg atccggatac tggtgcccaa ttgttttacc aatggagctg gggaaacgag
     6361 gagagtggtt ggctcggccc ctatgaatca gggcaatcga ttcaagcgat tcatcaatgg
     6421 tcgacagcag gggattatca ggtgaaggtg agagcgaaag ataaccttgg atctatgagt
     6481 gcttggtctg attcgataac aattcagatt gtcgaaggac ctcagatcga gattggaacg
     6541 atctcaggtg gaatgaaaat cactgctgag ataaaaaaca caggtattgt cgatgcctcg
     6601 catgtcaact ggtcgatcac cctgcaaggt ctggttctct tcggacaaga aaaaagtggg
     6661 actctcctgg gtatctctcc aggtgatagt ttagatgttt ctacaggatt gattcttggt
     6721 ctagggactg tcgatattac ag
//
LOCUS       DATMXJ010000148         4817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_13482, whole genome shotgun sequence.
ACCESSION   DATMXJ010000148 DATMXJ010000000
VERSION     DATMXJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13482"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VN377_03985"
     CDS             <1..1081
                     /locus_tag="VN377_03985"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27582.1"
                     /translation="STSRYHSGSSSFKSRYTNSDVSTMTTVNPILITPGMKLSFWCWY
                     NTENNYDDAFVEVSTDGRFYNILDIFTGSSGSWQYKEYDLGNHSGDSLFIRFRYTTDT
                     GTLQEGFYVDDITPVVDIATVTTLSSTITNDYYDITGKTNGTYYYRVKGHNSVRGWCD
                     FSTLEKILVSVGEDTTPPVTTCTLAGELEGDVYVSDVTVTLTATDDSGVDYTKYKLDD
                     GAWTMYTAPFVVSTNGNHTVTFYSVDTIGNTESEKNRSFTIQKEIPSVTITIKGGLGV
                     SATIKNTGTANLTDLDWTMNLDGKLIFVGKTKSGTIGSLDIGESVTINYFVLGFGKTG
                     IAMHVEATEATATGTVLLVFVIGVS"
     gene            1176..3059
                     /locus_tag="VN377_03990"
     CDS             1176..3059
                     /locus_tag="VN377_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27583.1"
                     /translation="MKKGLVGIIVAVVMIGGYGAVATSFVNQNSTEKTDTIVFSSVRL
                     VEEQSFTTIDLSEATSYLTTQGSYTIPVVTRVYTFPFLTKIKGVAVSFSEAQEIPLSK
                     PLKHAPEAIADTGADSTDVSLQSTSLNICPEQSYSYHMAAGRQNEDAVTFLAIHLYPV
                     QYLPSESILRCSKNAKITITYELPEARSATSSEQYELVIIAPAKFSEALQPLVTHKID
                     KGMTTKLVTRDDICNSVYFPAEGRDCAEEMKYFIKNAFDQWSTKYVLLVGGRSGGVTE
                     EKWWIPVRYSLLNDGGEGSYLTDLYFADLYDSNGSFSSWDSNNNGVFAEWTNTQRDVM
                     DMYPDVYVGRLSCTNVPQVKTMVKKIITYETTAYGAEWFKTFVGVAGDTYPEISDPYY
                     EGEMATNASFQQLESLGFTASMIWTSNGRFSDANSVIAEISKGCGFVHFSGHGNVLAW
                     STHPSRNESWVNGPNAFDMKKMSNGDQQPIVIVGGCHNAQFNTSLSNMLTGVLEDGLS
                     YFKSTRPFGAFWSYKWIPRCWSWAMASQTRGGAIAVIANTGLGYGEPGEQTLTQRGRH
                     LEWLFFKAYSDGKVNLGETHGTDLIYYMNEHPPMADQTDCKIVQEWALLGDPSLLIGG
                     YPS"
     gene            3148..4443
                     /locus_tag="VN377_03995"
     CDS             3148..4443
                     /locus_tag="VN377_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWR27584.1"
                     /translation="MQKILFVFVVVALLINSSVFLVSAQDQQSAGIAQSSDDATIIEM
                     INQINESLLLKYHSGLLKFGPRYTGSISCNLAAEYIYDEFQQMGLETEFHEWRYGGFH
                     SKNVVATLHGTDLDSTEEYILCGHYDTVADAPGADDDGSGTAAVLAIASVLRNYQFNH
                     TIRFITFSGEEVGTYGSFTYARDASHRGDNIVAVLNIDMIGYAGTTEGGRVLRFFPPT
                     RSSWIASDATSVSEKYNDIIDMKVVTLPAYRGADNQAFVDYGYDGVWIAHQDGYPWGH
                     SPDDTADHLNWSYYVKATKLMLAITAEFAQKPLDVQVFLHRPYEGYTYFFSYPIFESS
                     FGKKYFNGLRGSTFILGGRTVASAEVFSDEPIQYVVFCIDGYFMVWDSDPPYEWTIQG
                     WFTPIIGKHILDVYAFTTTGKVASDQMDITIFEFATSYK"
     gene            4491..>4817
                     /locus_tag="VN377_04000"
     CDS             4491..>4817
                     /locus_tag="VN377_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27585.1"
                     /translation="MRRRVGLLFIGIFILVVSFGVSSGTGAKVVLVTGFEPFGTYPIN
                     PSQVIAETLNGSTLHDAEIIGIILPVNFTTSVERAKEAIELYKPDVVISLGLNAKAEV
                     IEVEKIG"
BASE COUNT         1255 a         1021 c         1208 g         1333 t
ORIGIN      
        1 gtcaacctca cgataccact ccgggagctc aagcttcaaa tctcgttaca ccaattcgga
       61 tgtttctacc atgacgaccg tcaacccaat cctgattacc ccagggatga agctttcatt
      121 ctggtgctgg tacaatacag agaacaatta tgatgatgcg tttgttgagg tcagtaccga
      181 tggccggttc tacaatatct tagacatctt tacaggatca tcagggagct ggcaatacaa
      241 agagtatgat ctgggtaatc actccggaga ctccctcttc atccgattta gatacacaac
      301 cgatacaggg acattacagg aggggttcta tgtcgacgat atcacccctg tcgtagatat
      361 cgctacggta acgacacttt caagtacgat caccaatgat tattatgata tcactgggaa
      421 gaccaatgga acctattatt accgggtgaa aggccataac agcgtacgtg gatggtgtga
      481 ctttagtacc cttgaaaaaa tcttagtctc cgtaggggaa gatacgactc cgccagtgac
      541 cacctgcacc ttagcagggg agctagaagg agacgtctac gtcagtgatg tgaccgtcac
      601 cctcaccgca accgatgatt ctggtgttga ttatacgaaa tacaaactcg acgacggagc
      661 atggacgatg tacacagcac cctttgtcgt ttcaacgaat ggaaatcata ccgtcacctt
      721 ctactcggtt gataccattg gcaacacaga gtccgagaaa aaccgctcct ttaccatcca
      781 aaaggaaatc ccatcggtga ccatcacgat aaaaggtggg ctgggtgttt ccgcaaccat
      841 taaaaacaca gggacagcga atctgacgga ccttgattgg acgatgaacc ttgacggaaa
      901 actgattttt gtgggaaaaa caaaatcagg taccatcggc tcacttgaca taggtgaatc
      961 agtcacaatt aactatttcg tccttggctt tggcaagaca gggatagcga tgcatgtcga
     1021 ggcgactgag gcgaccgcga ctgggacagt tctcttggtc tttgtaatcg gggtatcgta
     1081 agttcaactc cttttttttc tttttttatc tcatttgttc gcaaagattt atatattatt
     1141 ggaggttttt attaaagcac attaggggaa aatctatgaa gaaaggtctt gtaggaatta
     1201 tcgtagctgt cgtgatgatt ggcggttatg gagctgttgc cacttcgttt gtaaaccaaa
     1261 atagtacaga aaaaacagat actattgtgt tttcatctgt tcgacttgtt gaagaacaga
     1321 gctttaccac tatcgatctt tctgaagcaa cctcatatct caccacccaa ggaagctata
     1381 ctataccagt tgtgacccgg gtgtatacgt tcccatttct gactaagata aaaggtgttg
     1441 cggtcagttt ttctgaggcg caagaaatac ctctctcgaa acctttgaag catgctcctg
     1501 aagcgattgc agacacgggg gcggactcca ctgatgtttc ccttcaatcg acttcattga
     1561 atatctgtcc ggaacaaagc tatagttatc atatggctgc tggaaggcag aacgaggacg
     1621 ctgtaacgtt tttagcgatt catctctatc cagttcagta cctaccttca gaaagcatcc
     1681 tgcgttgttc caaaaacgcg aagatcacga ttacctatga actcccagaa gcacgttcgg
     1741 caacatcctc tgaacaatac gagctagtga tcatcgctcc tgcgaaattc tcagaggcgt
     1801 tacaaccgct tgtcacccat aagattgaca aagggatgac gaccaagctt gttacgcgtg
     1861 atgatatctg caatagcgtt tatttcccag cagaaggacg agactgcgct gaggagatga
     1921 aatacttcat caaaaatgct tttgatcaat ggtcgaccaa atatgttctg ttggttgggg
     1981 gaaggagtgg tggtgtcact gaggaaaaat ggtggattcc ggttcgatac tctttactga
     2041 atgacggtgg cgaaggctcg tatttgactg atctgtattt cgctgatttg tacgacagca
     2101 atgggagttt ttcaagctgg gattcgaaca ataacggggt ttttgcggag tggacgaaca
     2161 cgcagagaga tgtcatggat atgtatccgg acgtgtatgt tggtcgtctg tcctgtacaa
     2221 atgttcctca ggtaaaaaca atggtgaaaa aaatcatcac ctatgagacg acagcgtatg
     2281 gggctgaatg gttcaagaca tttgtcggtg tcgctgggga tacctacccg gaaatcagcg
     2341 atccatatta tgaaggtgag atggcaacca atgcatcgtt tcagcagctg gaaagtcttg
     2401 gatttactgc atctatgatt tggacatcca acggtcgctt ttctgatgct aattctgtga
     2461 ttgctgagat aagtaaaggc tgcgggtttg tgcatttctc tggccatggg aacgttcttg
     2521 cctggtcgac tcatccttcg agaaatgagt catgggtgaa cggtccgaat gcttttgata
     2581 tgaagaaaat gtctaatggg gatcaacaac cgattgtgat cgtcggtggt tgtcataacg
     2641 cgcagttcaa taccagtctt tccaatatgc tgacaggggt attggaggat gggttgtcct
     2701 atttcaaatc gactcgacct tttggcgcat tctggtccta taagtggatt cctcgctgct
     2761 ggtcctgggc tatggcgagt caaacccggg gtggtgcgat cgcggtgatt gcgaatactg
     2821 gtctgggata tggtgagccg ggggagcaga cattaaccca gcggggacgg cacctggaat
     2881 ggttattctt caaagcctat tccgatggga aggtgaatct tggtgagacg catgggactg
     2941 atctgatcta ttacatgaat gaacatcctc ctatggcgga tcagaccgac tgtaagattg
     3001 tccaggaatg ggcgctcctt ggtgacccca gtctgctcat tggaggatat ccctcctaat
     3061 ctcttttttt ctttcttgtc atcgatattt tttataaaga aatatttata taatcaaagg
     3121 agaatcatat cacataggat gtgacgtatg caaaagatcc tgttcgtgtt cgttgttgtt
     3181 gctttgctta tcaattcatc cgttttcctc gtatcggccc aggatcaaca gtccgctgga
     3241 atcgcccaat cgtctgatga tgcaaccatc atcgaaatga tcaaccagat caacgagtca
     3301 cttttattga agtaccattc tgggttgctg aagtttgggc ctcgttacac cgggtcgatc
     3361 agttgtaatc ttgctgcgga atatatctat gacgagtttc agcagatggg tcttgagact
     3421 gagtttcatg aatggcggta tggtggtttc catagtaaaa acgtcgtggc gaccctgcat
     3481 ggaacagacc tggatagcac ggaagagtac atcctctgtg gtcattatga cacggtcgct
     3541 gatgcccccg gggcagatga tgacggctct gggaccgcag cggtccttgc gattgcctct
     3601 gtcctgagga actatcagtt taaccatacg atccggttca tcacgttctc cggtgaggaa
     3661 gtcgggacgt acgggagttt tacctatgcc agggatgcat cccatcgtgg tgataacatc
     3721 gtcgctgtcc tcaatattga catgatcggt tatgcaggta cgacagaagg aggaagagtc
     3781 ctgcggtttt tcccgccaac gcgttcttct tggattgcga gtgatgcaac cagtgtcagt
     3841 gagaaataca atgacatcat tgatatgaag gtcgtgacgc tcccagcgta ccgcggagct
     3901 gataatcagg cgtttgttga ttatgggtat gacggggtct ggatcgcaca ccaagatggg
     3961 tacccctggg gtcattcacc ggatgatacc gctgatcatc tgaactggtc gtattatgtg
     4021 aaggcgacta aactgatgct tgcgatcacc gctgagttcg cacaaaaacc acttgatgtc
     4081 caggtcttcc tgcatcgacc ctatgagggg tacacgtatt tcttttcata cccgatcttt
     4141 gagagttcat ttgggaagaa atattttaat gggcttcgag ggtccacgtt catccttggg
     4201 ggaaggacag tggcgagtgc agaggttttt tccgatgaac cgattcaata cgttgtcttc
     4261 tgtatcgatg ggtatttcat ggtttgggat tcagatcctc catacgaatg gacgatccag
     4321 gggtggttta ctccgattat agggaagcat attcttgatg tctatgcgtt tacgacgacg
     4381 gggaaggtcg catctgatca aatggacata accatttttg aatttgcgac atcgtataaa
     4441 taagagccga agaggaatag ttttatagga gaagtacata taaaaattgt atgagacgtc
     4501 gagtgggctt gctcttcatt ggaattttca ttctggtggt ctctttcggg gtatcaagtg
     4561 ggactggtgc gaaggtcgta ctggtgactg gatttgagcc ttttgggaca tatccaataa
     4621 acccgtcgca ggtcatcgct gaaacgctga atggatctac ccttcatgat gcagagatca
     4681 tcggaatcat cctccctgtc aatttcacga catccgttga aagagcaaaa gaagctattg
     4741 agttgtacaa gccggatgtg gtgatcagtc ttggtctgaa tgcgaaagct gaggtcattg
     4801 aggtagaaaa aattggg
//
LOCUS       DATMXJ010000149         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_8130, whole genome shotgun sequence.
ACCESSION   DATMXJ010000149 DATMXJ010000000
VERSION     DATMXJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8130"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VN377_04005"
     CDS             <1..972
                     /locus_tag="VN377_04005"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HWR27586.1"
                     /translation="HQDVIWVTCDGDMGNNELVAAGIKNIQIVYSPSGATTSANDTKN
                     ACKKFLESPPDLLVFCGGDGTARDIFTTIDKKIPLLGIPAGVKMHSGVFGITTKATAN
                     MLQEFINKHLTIGDAEIMDLDEGLYRKGEWKVRLYGIAKGIIEPTYIQVGKSCFESIS
                     DDEVKDELAEHVNDEMEKHTNWMFLFCTGGTIDHIAKKLNIEHTLLGIDAVYQKKLLA
                     KDVNEEQILQLLKKYPKTKILLSPIGAQGFILGRGNLQLSPTVIKKIGIENIIVVSTP
                     AKLIHTPILRVDTGDKQLDHRFANQGYYMVVIGYRLSRVVKLQTNSF"
     gene            1028..2227
                     /locus_tag="VN377_04010"
     CDS             1028..2227
                     /locus_tag="VN377_04010"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HWR27587.1"
                     /translation="MMTQITKRNANKFLKDEYDELVKNHFEWNIRILESGSKPHSIVN
                     GKEVIMMCSNNYLNLSNHPRLIKGAIDAANKYGAGSGSVRAIAGTMKIHTEVEKRLAK
                     FKRTESSLIYQTGFAANSGLIPQLVGEGDIVISDELNHGSIIDGVRLTKSDRAIYKHR
                     DMSDLERVLKDADKKYRRILIITDGVFSMDGDIAPMDKIVKLANEFGAMTYVDDAHGE
                     GVIGEDGRGIGAHFHIEGKIDVEMGTFSKAFGVVGGLIAGSQDLVNFAYNKSRTWLLS
                     GSHPPAVSGAQLAAIDVLETEPEHVKRLWDNTKYFKKELNSLGFNTGGSETPITPIIV
                     GESGKAKELSNKLYDEGVYALPIVFPMVARDKARIRTMMNAGLTKNDLDFALDKFEKL
                     GKQLSII"
     gene            2347..>2544
                     /locus_tag="VN377_04015"
     CDS             2347..>2544
                     /locus_tag="VN377_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HWR27588.1"
                     /translation="MTEDLKKTGTTILGMVCKDGVVIATEHRATMGTLIAHKATKKLY
                     QIDNHLALATAGLVGDLQVLSR"
BASE COUNT          801 a          545 c          568 g          630 t
ORIGIN      
        1 caccaagatg ttatctgggt gacgtgcgac ggagatatgg ggaacaatga gttggtggct
       61 gctgggataa aaaacataca aatcgtctat tcaccttcag gggcgacaac ctcggcaaat
      121 gacaccaaga atgcctgtaa aaaattccta gagtcccccc ctgatttact cgtcttctgc
      181 ggtggtgacg gaaccgcccg agatatcttc accaccatcg ataaaaaaat cccgctcctc
      241 ggcataccag ccggcgtcaa aatgcactcc ggtgtctttg gaatcaccac aaaagcgact
      301 gcaaatatgc ttcaggagtt catcaataaa catctgacca ttggtgatgc tgaaatcatg
      361 gaccttgatg agggactcta ccgaaaaggc gaatggaagg ttcgtttata tggcatcgca
      421 aaaggcatca tcgaaccaac atacatacag gtaggaaaat cttgctttga atccatctca
      481 gatgatgaag taaaagacga gctggctgaa catgtcaacg acgaaatgga aaaacatacg
      541 aattggatgt tcttattttg cactggtggc accatcgatc atatcgcaaa gaagctcaac
      601 attgaacaca cactgctggg tatcgatgca gtgtaccaaa aaaagctcct agcaaaggat
      661 gtaaacgaag aacaaattct gcaacttcta aaaaaatatc ctaaaacaaa aattcttctc
      721 agccccattg gggctcaggg gtttatcctt ggacgcggga accttcaact cagcccaacc
      781 gtcattaaga aaataggaat cgaaaacatt attgtggttt caacccctgc aaagctgatc
      841 catactccta tcctacgggt agatacagga gataagcaac ttgatcatcg tttcgctaac
      901 caggggtact acatggtagt tatcggatac cgtcttagta gagtcgtgaa gcttcagacc
      961 aacagctttt aatatccgaa tcttatttcc tgaccccctc gggggacgta tcattcttgg
     1021 aggtaacatg atgacacaaa ttacaaaaag aaacgcaaac aagtttttaa aagacgaata
     1081 cgatgaatta gtaaaaaacc attttgagtg gaacatccgt attctcgaga gtgggtcaaa
     1141 accacattcg attgtcaatg gcaaagaagt aatcatgatg tgttcgaaca actaccttaa
     1201 cctaagcaac catccccgtc tgatcaaggg ggctatcgat gctgcgaata aatacggtgc
     1261 tggttctggt tcagttcgtg cgattgcagg aaccatgaaa atccacacgg aagtcgaaaa
     1321 acgactcgcg aaatttaaaa gaactgagtc ttccctaatt tatcaaactg gttttgctgc
     1381 aaattcaggc ttgattccac agcttgttgg tgaaggcgat atcgtcatca gcgatgagct
     1441 caaccatggg agcatcattg acggtgtgcg gcttaccaaa tcggatcgag cgatttacaa
     1501 gcatcgtgac atgagtgacc ttgaacgagt gttaaaggat gcggataaga aatatcgtcg
     1561 tatcctcatc atcaccgatg gtgtcttcag tatggatgga gacattgccc ctatggataa
     1621 aatagtgaaa ctcgccaatg aatttggagc gatgacctat gttgatgatg ctcatggaga
     1681 aggagtcatc ggggaggacg gccgtgggat cggagcacat ttccatatcg aaggaaaaat
     1741 cgatgtcgaa atgggaacgt tcagtaaagc cttcggtgtg gtcggtggtc ttatcgccgg
     1801 cagtcaagac ctggtcaact tcgcatacaa caaatcccgg acctggttac tttcaggttc
     1861 acatccacct gctgtgtctg gtgctcaact tgctgctatc gatgtgcttg aaacagaacc
     1921 cgagcatgtc aaaagactct gggataatac aaaatatttc aaaaaagaac tgaactcctt
     1981 agggttcaac actggaggga gtgaaacacc tattacccca atcattgttg gagaatctgg
     2041 aaaagcaaaa gagctaagca acaaactcta cgatgaaggc gtctatgcac tacctattgt
     2101 attcccaatg gtcgcacggg ataaagcccg cattcggact atgatgaacg caggtcttac
     2161 gaagaatgat ttagacttcg ctttagataa attcgaaaaa ctcggaaaac aattatcaat
     2221 tatctaagcg aaaaagagga gaatcgtgat gtacgaatct cctcacatta acactccaca
     2281 aagtatatat aaaagatata ttattcgtta tccagtgttt ttctggtgtt tactggagag
     2341 taatttatga cagaagattt aaagaaaacc ggtaccacaa ttcttggaat ggtatgcaaa
     2401 gacggtgttg ttattgcgac agagcataga gcgaccatgg gtacgcttat tgctcataag
     2461 gctacaaaga aactgtatca aatcgataat catctcgcac ttgcgaccgc tgggcttgtc
     2521 ggtgacctgc aggttctttc tcga
//
LOCUS       DATMXJ010000150         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55465, whole genome shotgun sequence.
ACCESSION   DATMXJ010000150 DATMXJ010000000
VERSION     DATMXJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55465"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="VN377_04020"
     CDS             <1..2187
                     /locus_tag="VN377_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27589.1"
                     /translation="TPPSPIFSQCILTGQTYATVQLPDEGISTVIGEAQLPAISRFIE
                     IPQGATPQIVVESVSWETTSLEALQLPSEIIPVQPSLVKIDGASVAFTMNELYYAMNA
                     LLPSSFATVSSIGELRERNIAFLNISPVQYNPASGELRIMTYCVLRINLPGSDLALTT
                     QKIDRYTTPSFEQMFETSFINYGSLQGTPSIGPRQEGYLIIVYDDYFDEIQPLVSWKD
                     SKGFDVTVTKTSEIPSSPTKENIKAYIVDAYNNWPIPPSYILLVGDVEQIPAWTGSDT
                     GTCTDLYYVTIDAGNYFADVIISRFPAATAAQVTTMVDKTIFYEVGVFPNESWIKKAA
                     FMASSDNYQVSEGTHNYVIDNYLLPNNYTCDKLYSHTYGATTQQVRDALNNGRSLAIY
                     SGHGGTDYWADGPVFYQSDVNGLINNGMYPFVCSHACLTNQFTESECFGETWLRAPNK
                     GGLAFWGATTYSYWDEDDILERSMFKAWWEDNLETIGGMTNKALFYLYQYYSGGGMSQ
                     YYFEEYNVLGDSSVKIWRGQTNINTPPTVPETPNGPSTGEIGIEYTFTTSTTDAQNND
                     VYYLVSWGDSVTDWIGPYASGATVELKHTWNFVGDYSIKVKAKDSEGLESDWSEASTI
                     SIVAVPRIEIGEITGSFGGISAKIMNVGAGEATNVDWSINLQGGLVLLGRENTGTFTK
                     IMPGFAPKANTGFVFGLGSVDILITAGDVEKTVTARLIGPFFLGLS"
BASE COUNT          647 a          494 c          527 g          634 t
ORIGIN      
        1 acaccacctt ctccgatatt tagccaatgc atcctcacag ggcaaaccta tgctactgtg
       61 caactacctg atgaagggat ctccacagtg attggtgaag cgcaactacc cgccatctcc
      121 cgattcattg agattcctca aggtgcgaca cctcaaattg ttgttgaatc ggtttcctgg
      181 gaaacaactt cattggaggc attgcagctt ccctcggaga taatcccggt acaaccatca
      241 cttgtgaaaa tcgatggagc atccgttgca ttcactatga acgagctcta ttatgcaatg
      301 aatgcattgc ttccttcctc ttttgcaacg gtgagttcga ttggggaatt gagagaacgt
      361 aacattgcat ttcttaatat ttctccggtg caatataatc ctgcatctgg agaactgaga
      421 atcatgactt attgtgtcct gcggatcaac ctccctggaa gtgacctggc gttaacaaca
      481 caaaagattg atcgctatac cacgccatcg ttcgaacaaa tgtttgaaac atcattcatc
      541 aactatggtt cgttacaagg tactccaagt atcggaccaa gacaagaagg gtatctgatt
      601 atcgtgtatg atgattattt tgatgaaatt caaccgctag tttcttggaa agactccaag
      661 ggattcgatg tcactgtcac gaaaacatca gagattccga gtagccctac taaagagaat
      721 atcaaagcat atatcgttga tgcctataac aactggccga taccaccttc ttatattctc
      781 cttgtcggag atgtcgagca gattcccgca tggacggggt cagatacagg gacctgcact
      841 gatttgtatt atgtcaccat tgatgcaggc aactattttg ctgatgtgat tatcagtcga
      901 ttcccagccg cgaccgctgc acaggtgacg acgatggtgg ataaaacaat cttttatgaa
      961 gtcggagtgt tcccgaatga atcttggatc aaaaaagctg cttttatggc atcaagtgat
     1021 aactaccaag tctccgaagg aacccataac tatgtcatcg ataactacct tcttccgaat
     1081 aattatacgt gcgataaatt gtacagccac acctacggtg caacgactca gcaggtgaga
     1141 gatgcactaa acaacggacg aagtttggca atctattccg gtcatggagg aactgattac
     1201 tgggcagatg gtcctgtctt ttatcaatct gacgtcaatg gattgatcaa caatggaatg
     1261 tatccgtttg tctgtagcca cgcgtgtctg acgaaccaat ttacagagag cgaatgtttt
     1321 ggtgaaacat ggttgcgcgc accaaacaaa ggcggtttag cgttctgggg tgcgacgacg
     1381 tattcatatt gggatgaaga tgatatttta gagagaagca tgttcaaggc atggtgggaa
     1441 gacaaccttg agacgattgg tggtatgacg aataaagctc tcttctacct ctatcaatac
     1501 tatagtggtg gtgggatgag ccaatattat ttcgaagagt acaatgtcct tggggattcc
     1561 tccgtaaaaa tctggcgtgg tcaaaccaac atcaacacac caccgacagt ccctgaaaca
     1621 ccaaacggac catcaactgg agaaatcgga attgaatata catttacgac cagcacaacc
     1681 gatgcacaga acaatgatgt ctactatttg gtgtcttggg gtgattcggt aaccgactgg
     1741 atcggcccct atgcttcagg agcaacggtc gaattgaaac atacatggaa ttttgtcggg
     1801 gattacagta ttaaagtgaa agccaaggat agcgaaggct tagaaagcga ctggtcagaa
     1861 gcatcaacca ttagcattgt tgcggttcct cgcattgaga ttggggaaat cactggtagt
     1921 ttcggaggta tatcggcaaa gataatgaac gttggagccg gtgaagcaac aaatgtcgac
     1981 tggtcgatta atcttcaggg cggcctggtc cttcttggac gggagaacac tggtacattt
     2041 acaaagatca tgcctggttt tgctccaaaa gcgaacactg gttttgtgtt cgggttaggc
     2101 tcggtggata tccttatcac cgctggtgat gtcgaaaaaa ctgtgaccgc tcggttgatt
     2161 gggccatttt tcttgggttt gagctaaaac ccccctttct ttcttcttct atgatacttc
     2221 ttgaggatcc tgaggagtca acctaaggat tctcagattt atcatgccat ctttttttca
     2281 atttaaagag ttattattaa tt
//
LOCUS       DATMXJ010000151         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55474, whole genome shotgun sequence.
ACCESSION   DATMXJ010000151 DATMXJ010000000
VERSION     DATMXJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55474"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VN377_04025"
     CDS             <1..1147
                     /locus_tag="VN377_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27590.1"
                     /translation="TYRWYVNVTDGTHWTREIFEFQTGFPTNFDPFTFGWQYRKQITI
                     DHTQVTGDLVNFTVLLSTIDPDLIKAQTDGEDILFMNSTGAAIKLHHEIEVFNQATGA
                     LLAWVNIPSLSSVEDTALYLYYGNPNCVDQEYPEKTWDSNYILVYHMNDLTTSIVKDS
                     TTNNNGGTKKGANEPIQTIGKIGYAQYFDGVGDYITTNSAIALGSNPWTYECWYKADS
                     YGHSEDPYTDKYSEISTVQPSTSVPYLCIDISNRESKDRIRTYMDNVPSHGYSYTNAL
                     ASPFDNNTRQITVVRDNANTDFKIYLNGATDYTVAGAVDTSYSITTNKLRIGGSAYNP
                     SRVCFEGFIDEIRVSKIVRSSQWISTSFNTQNNPEDFISIGTEESGP"
     gene            complement(1284..1673)
                     /locus_tag="VN377_04030"
     CDS             complement(1284..1673)
                     /locus_tag="VN377_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27591.1"
                     /translation="MSYKKTSVAEDIWEQSGKRSMTCPQCKGFLTVVQVEPIYDQDNA
                     YTPYRTVVECASCSFRMVTESFTILGGVKDFDNEYVEIGSWGPSGSRDLSRFKHSISS
                     NLLSELKKSQELVEFLIVNEHVVQVIG"
     gene            1829..2617
                     /locus_tag="VN377_04035"
     CDS             1829..2617
                     /locus_tag="VN377_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWR27592.1"
                     /translation="MKRITLLTDFGTKGAYVAQMKGVVSSITDARIVDITHEVSSHNI
                     REGAFLLWTTAPFFPVGTIHVAVVDPGVGTERKGLIITTKKQILVGPDNGILLPTAHL
                     LGDFIVYEITNPRYMIHPLSQTFHGRDIFAPVAGYIARGVPFSEIGTKTTHYVDLQFP
                     LGEHRGDRIVGKVLYVDRFGNLITNIPHDILPSDIGGKKISVLSGDRRWDQVPFVTSY
                     GYAKQGELLVTFGGNHFLEISVNQGDAAQSLGMQVDTEVSLVFG"
     gene            complement(2614..3165)
                     /locus_tag="VN377_04040"
     CDS             complement(2614..3165)
                     /locus_tag="VN377_04040"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWR27593.1"
                     /translation="MKVQGTIEKYYHYAFPMQTVLVTCNDGKGNTNIITLAWHTPISR
                     NPPLYGISISPKRHSHALIKKSKEFVINFIPYSQVEAAEYCGTHSGRNTNKLCQTGLT
                     LIPAQKLSTPLIKEGYAHLECTLVKNLSLGDHTLFVGEVVAVSADENAFQNELLRTDR
                     IHPVYYIGQNAYTTLDRVKRKTF"
     gene            complement(3180..>3425)
                     /locus_tag="VN377_04045"
     CDS             complement(3180..>3425)
                     /locus_tag="VN377_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HWR27594.1"
                     /translation="PVNNETMDEKTRVQKDIVMFEDNIKHINQNTLTDNQKKTIELAT
                     QYYEDTKYYTNKKDYFTAFGCINYAHGLLDAILKTQQ"
BASE COUNT          891 a          648 c          804 g         1082 t
ORIGIN      
        1 gacatatcgt tggtatgtga atgtgactga tggcacacat tggacacggg aaatattcga
       61 atttcagacc ggatttccta ctaattttga tccctttaca tttgggtggc agtaccggaa
      121 gcagatcacc attgatcata cacaagtcac tggtgacctt gtgaatttca cggttctcct
      181 tagtacaatt gatcctgatc tcataaaggc acaaaccgat ggggaagaca tcctttttat
      241 gaacagtact ggtgctgcaa taaagctcca tcatgaaatt gaggtcttta accaggcaac
      301 gggggctctt ctcgcttggg tgaatatccc atcgctttct tcagttgaag atactgcgtt
      361 atacctgtat tatggaaatc cgaactgtgt cgaccaggag tatccagaaa aaacctggga
      421 ttccaattat attctcgttt atcatatgaa cgatttaacg acatctattg taaaagatag
      481 cactaccaat aataatggtg gaacaaaaaa aggagcaaat gaaccaattc agacaattgg
      541 aaaaatagga tatgcgcagt acttcgatgg agtgggcgat tacattacaa caaacagtgc
      601 tattgcactt ggtagtaatc cctggacata tgaatgctgg tataaagcag acagttatgg
      661 ccattcagaa gacccatata cagataaata ttctgaaata tctacagtac aaccatccac
      721 atctgttcct tatttatgta tcgatatctc aaatagagaa tcaaaagaca gaattagaac
      781 atacatggat aacgttccca gtcatggata ttcatacaca aatgcattgg cgagtccatt
      841 tgataataat acaagacaaa ttacagtggt tagggacaat gctaataccg acttcaagat
      901 ttatttgaat ggtgcgacag actatactgt tgctggtgca gtagatacct cttattctat
      961 aaccacaaac aaattgagaa tcggtggatc tgcatataat ccaagtagag tgtgttttga
     1021 gggttttatc gatgaaatac gagtatccaa aatagtccgt tcatcacaat ggatttcaac
     1081 atcttttaac actcaaaata atccggaaga ctttatatct attggaacag aagaatcagg
     1141 accgtaaatt tctttaatct acagagacca gtacaaataa tctttaaaaa agaaatgttt
     1201 attttattat taaaatcaga accatttttt gaaagtaatc ttttggagat ttaaaaatcg
     1261 gttcatctct tttgttgctc tgcttatccg atgacttgga cgacgtgttc attgacaatc
     1321 aggaattcga cgagttcttg tgattttttc aattcactaa ggaggttgga gctgatggaa
     1381 tgtttgaagc gtgagagatc gcggcttcca ctcggccccc agctgccgat ttccacgtat
     1441 tcgttgtcaa aatcctttac acctccgaga atcgtgaagg actctgtgac catgcggaat
     1501 gaacagctcg cacattcgac cacggtcctg tagggggtgt aggcattgtc ttggtcatag
     1561 attggttcga cctgtactac tgttaagaat cctttacact gagggcaggt catgcttcgt
     1621 ttcccgcttt gctcccaaat gtcctcagcg acacttgttt ttttataact catctctccg
     1681 ggctccattc cttcgtactt ctccaatctg tattaattat acacgcttct tccatatata
     1741 gttttgcttc aattggagtc agtgcggatg ataaagctct tgtatgaatg aataatattg
     1801 gataaatatt cgagttgagg tacgttacat gaagaggatt acgttactta ctgattttgg
     1861 tacgaagggg gcgtatgttg cacagatgaa aggggtggtt tccagtatca ctgatgcacg
     1921 gattgttgac atcacccatg aggtttcttc gcataatatc cgggagggtg cgtttctgtt
     1981 atggacgacc gcaccgtttt tccctgtggg tacgattcat gttgcggttg ttgacccggg
     2041 tgtaggtacg gaacgaaaag gattgatcat cacgacaaaa aaacagatac tagttggccc
     2101 ggataatggt attttgttgc caacagcgca tcttcttggg gattttattg tctatgagat
     2161 taccaaccca cgatacatga ttcatccgtt atctcagacg ttccatggtc gtgatatctt
     2221 cgcccctgtc gcaggttata tcgcgcgtgg agtcccgttt agtgagatag gtacgaagac
     2281 gacacattat gtggacctgc agtttccttt gggggagcat cgaggggacc ggatcgtcgg
     2341 taaagtcctt tatgttgacc ggttcgggaa cctcataaca aatattcctc atgatattct
     2401 tccaagtgat atcgggggga aaaagatttc tgtactgtct ggtgatcgtc gatgggatca
     2461 ggttccgttt gtcacttcgt acggatatgc aaaacagggg gaactccttg tcacttttgg
     2521 gggtaatcat ttccttgaaa tcagcgttaa ccaaggtgat gcagcccaga gtttggggat
     2581 gcaggttgat actgaggttt ccttagtttt cggttagaac gtctttcttt ttactctgtc
     2641 aagtgtcgtg tacgcgtttt gtccgatgta gtagactgga tggatgcggt ctgttctgag
     2701 gagctcgttt tgaaaagcgt tttcatcagc tgagactgcg acgacttccc cgacgaaaag
     2761 cgtatgatca ccaagagaca ggttttttac gagtgtgcat tccaggtgtg cgtagccttc
     2821 tttgatgagg ggtgttgaga gtttctgggc aggtatgagg gttaatccgg tttggcagag
     2881 tttattggtg tttcttccag aatgtgttcc gcagtattcc gctgcctcga cctgggagta
     2941 cgggataaaa ttgatgacga attccttgct tttttttatg agcgcatggg agtgtcgttt
     3001 cggggaaatt gagatcccgt agagtggtgg gttccgtgag atcggggtgt gccaggcaag
     3061 ggtgatgatg tttgtgttcc cttttccatc gttgcaggtg actaacacgg tttgcatggg
     3121 gaaggcgtaa tggtaatatt tttcaatggt tccttggact ttcataagac catcctcttt
     3181 tattgttggg tttttagaat cgcatcgagc agtccgtgag cgtagttgat gcagccaaac
     3241 gcggtgaagt aatctttttt gtttgtataa tattttgtgt cttcgtagta ttgcgttgct
     3301 agttcgatgg ttttcttttg gttgtcagtg agtgtgtttt ggttgatatg tttgatgtta
     3361 tcctcgaaca ttacgatgtc tttctgtact ctggtttttt cgtccatggt ctcgttgttt
     3421 acggg
//
LOCUS       DATMXJ010000152         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_65963, whole genome shotgun sequence.
ACCESSION   DATMXJ010000152 DATMXJ010000000
VERSION     DATMXJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65963"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VN377_04050"
     CDS             <1..1549
                     /locus_tag="VN377_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27595.1"
                     /translation="TNHPTSPLPLENLNWWNTSWPYRKLITIDHTKVDSSLSNFPVII
                     SIPSDSDLANKAQADGNDIVFVLYSDDTIKLNHEIEYYNGDTGMLYAWVNITSLSSST
                     NTKIWMYYGNADSGSQQNKYGTWNSKYKAVYHLDENGPPYVDSTSNKNNIATANVNGT
                     GMAQSGGIYKWQHFNGTSNRFVIPFSLSGAYTLEAIANTDSLASSQVLIGYGLTTSSP
                     YPGVAMGISSTSDAIFTGNGNLPKALSPIASYVTEKTTHWWSDVYRSNTSLRFYLDGI
                     NYTSSLSNVNYFNDADGYYTIGARFTSGSYGLFWKGYIDEVRISSGTWNASWMKTCYN
                     NQHNPESFYSIGAEQIQLPNPPPFTGPSWGLINVDYTFCVTVTNPNGDNLYCIWDWGD
                     GDFSEWLGPYSSGEMVCASHPWSQKGTYGIRVKLKDVYGQESGWSNPQIITIYELKQA
                     IIFGSYANKITDGGFISIHAVNLRMVLFKPFQFDHYVQGENITFSNTFKGILLKQLII
                     GQFDVVK"
     gene            2299..>5787
                     /locus_tag="VN377_04055"
     CDS             2299..>5787
                     /locus_tag="VN377_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWR27596.1"
                     /translation="MTHILWIILVISSLSTFSTITSIAAPTNQPLVFGNPSPANGSTG
                     NWWSPPPYGEWIPWNIPISDPEGNRISWIIQCSNGQTTSGTNTFNGSKYLAISPLSYS
                     TTYKIWVNATDPAGSGLWTRKWYTFTTRGDGNNSPVLPRAPSPANGSSGNPLSLSWSI
                     RINDLEGDLFSWSLQCSNGDENSGLSASNGTKSLVLSGLAYSTSYKVWVNATDPDGSG
                     LTIRRWFTFTTKANDPPVFDSPSLTNNSIGNSLGFTWGIPINDPDGNSFNWRIQCSNG
                     QSANANSASNGTKSLSLSGLASSTMYTVWVNATDPAGSGLWTRQWFTFITQGIGGNSP
                     PEFGTPSPANGSSGNRLYFNWNIPVNDPEGTDFSWTIQCNNGQTTSGTSANNGTKSLF
                     LSGLAYSTSYKIWVNATDPTGSTQYTRKWYTFTTETIIPFNIPTPNSWVFVTSASGES
                     RVQPSVADINNDGRMEIIRSGINGIVVYDGKTGTILWDNITTMWDSHNPLEIIDLNND
                     GYLDVISSYNTGTRALSGEDGSQLWYNANAPLYNKHAVAGDINADGYPEVFVCAAGAE
                     DGTPEGWIKGLTHDGTIFAQVHVYFPCYGGLSLGDTDFDGVYEVYLCERNIGYDGNVV
                     GKGVAAFWASNLTQRWSHPEMLSSSHCPTLVDTNKDGILEVVALAQTGSNGIAVYNSS
                     DGTVIHQSNIPGVRCHSQPTVYDIDGDGNLEIIVGGGSDAWGKPAIWDLYTWSLEAWL
                     PFNCWEPPAIVDLNGDGRVEILECTITNISIFDDNYVFRGSIPLDNNRSGYGWYGMSM
                     IIAQDIDNDNKLELVINRNTRTYAYDTDGAAPTPLASSQYMYYSPLRGRYPYSTPYGA
                     TAPWAINEYPANESTNIPFNPQLSVYIYDNQEDSMNIIFRTNASTGVWHNIETYSNIH
                     EGTYTADTTDMDTSLTYYWWSITVTDSTAKTTYKLYKFKTINGEPEPPYTPSNPFPAN
                     ESTGVPVTADLGWTGGDPNGDPVTYDVYFGTTNPPAKKVYNQSALLYNPSTMSNITHY
                     YWKIIAWDNHSTSTEGPLWEFTTGLKVNNPPNPPSNPSPANGATKVVISAKLSWTGGD
                     PDGDTVTYDVYFGTTSQPPKVNTNQSALTYNPGALAYDTTYYWNIIAWDNHSANVSGT
                     TFDPWNFTTKSPGGGGG"
BASE COUNT         1805 a         1194 c         1240 g         1548 t
ORIGIN      
        1 aacaaatcac ccgaccagcc cattacccct ggaaaatctc aattggtgga atacaagttg
       61 gccatacagg aaattaatta ccatcgacca taccaaagta gattcatctc tgagtaactt
      121 cccagtaatc atctcaatac catctgattc agatttagca aataaagcac aagccgatgg
      181 gaatgatatc gtgtttgttc tctactctga tgacaccata aaactcaacc acgagataga
      241 atattacaat ggtgatactg gcatgttata tgcctgggtg aatatcacta gtttgagtag
      301 ctcgaccaat acaaagatct ggatgtacta tggcaacgct gattcgggga gtcaacagaa
      361 taaatatggg acttggaact cgaaatataa agccgtgtat catcttgatg aaaatggacc
      421 tccgtatgtt gatagcacca gcaataagaa caatatcgcg acagcgaacg tgaacgggac
      481 agggatggct caatctggtg gtatttataa atggcaacat ttcaatggga caagcaatcg
      541 gtttgtgata ccattttctt tgtcaggagc gtacacgtta gaagcgatag caaatacgga
      601 ttcattggca tcatcgcagg ttttaatagg atatggtttg actacatcga gcccctaccc
      661 tggagtagct atgggtattt cttctacgag tgacgctatt tttactggta acggtaatct
      721 tccaaaagct ctgtctccca ttgcatccta tgtcacagaa aaaactactc attggtggtc
      781 tgatgtgtat agatcgaaca caagtcttcg cttttatttg gatgggataa attatacttc
      841 ctccttgtcg aatgtgaatt actttaatga tgccgatggt tattatacga taggagcgag
      901 atttacttct ggtagttatg gtttattttg gaaaggttat attgatgagg ttcggatatc
      961 gagtggtact tggaatgcga gttggatgaa aacctgttac aacaaccaac ataatcctga
     1021 atccttttat tccatagggg ccgaacaaat acaacttccg aacccaccac catttactgg
     1081 cccatcgtgg ggtcttataa atgtcgatta caccttctgc gttacggtga ctaacccgaa
     1141 cggggataac ctctattgta tatgggattg gggcgatggg gatttcagcg aatggcttgg
     1201 tccctattcc tctggagaaa tggtctgtgc atctcatccg tggtctcaaa agggaaccta
     1261 tgggattcga gtgaaactca aagatgtcta tggtcaagag agcggttggt cgaatcctca
     1321 gattatcacg atttacgaat taaagcaagc gattattttc ggcagttatg caaataaaat
     1381 cactgatggg ggattcatct ctattcacgc ggtaaatctc agaatggttc tcttcaagcc
     1441 atttcaattc gatcactatg tccaagggga gaacatcaca ttttcaaata ctttcaaagg
     1501 catactcttg aaacagctca tcattggaca gtttgatgtt gtaaaataat taatacacgg
     1561 gagcggaaaa acgtaagacc gatatggttc gaaaatcaaa ggaaggtcag cctccaaaaa
     1621 tcaaagaagt gactgatagg tatagagatc aatcgtccca ctgggaagaa gacaaataat
     1681 cttttctcct ttttcttata tcattaattc acacaaaaaa tcattgagtt tgaaagcgtg
     1741 tgcattcttc ctttttggat gaggaacaca ccgaaactat gttttcgtac tcgatacgtg
     1801 ttgagtaaaa aggacactga agtgtaaaac cggtaagagt tgtcaaagaa attatatatc
     1861 ccagtgagac aacttttata ctgagattat ggttgaagtg gtaaaaatat cgaaaaaagg
     1921 gagactttta cgaattaaag aagtctctga tatatatcgc gaccagcctt ataaatggga
     1981 tgcgaagaag atcacggaat aaaggagaat gagatacatg gttactttta aattaatgaa
     2041 tggagaaagg tttattggta ccattaaaga atcccgtcat tcagtgttgg taatagaaga
     2101 ttttaaaaca ggaaagataa gagaaattcc tgtaatacat attctttcaa ttgtttatta
     2161 aatattatcg agctttttct ttgaacatgg aaattgaatc aaaaactata taactttaat
     2221 aaattatcat atcaaaatag ttcttgaata aataaaagaa ataaccaggt ggaaacaatg
     2281 aatttaaaga aaaagataat gacgcatatt ttatggatca tcctcgtaat ttcatcactt
     2341 agtacttttt caacaatcac atctattgca gcaccgacca accaaccact ggtgtttggt
     2401 aatccatctc cagcgaatgg ttctacaggg aactggtgga gtcctcctcc ctatggagaa
     2461 tggattcctt ggaatattcc gatcagcgat cctgagggaa atcgcatttc atggatcatc
     2521 caatgcagca acggacaaac cactagtgga actaatacat tcaacgggtc caaatatcta
     2581 gcgatctcac cacttagcta ttcaacgacg tacaagattt gggtgaatgc aactgatcca
     2641 gcagggagtg gtctatggac acgtaaatgg tatacgttta caacacgagg agatggtaat
     2701 aattcaccgg tgttaccacg tgcaccttct ccagcgaatg gttcttcagg aaacccattg
     2761 agtctgtcct ggagtatccg gatcaatgat ctggaggggg atctcttttc ttggtctctt
     2821 caatgcagca acggagacga gaatagtgga ctaagtgcat cgaacgggac aaagtctctt
     2881 gttctctctg ggctcgcgta ctcaaccagc tataaagtct gggtaaatgc aaccgatcca
     2941 gatgggagtg gtctcactat ccgcagatgg tttacgttta cgacaaaggc gaacgatcca
     3001 ccagtttttg attcaccttc tctgacgaat aattctattg ggaactcgtt gggtttcact
     3061 tggggaattc ctatcaacga cccggatggg aatagcttca attggcggat acagtgcagt
     3121 aatgggcaat ctgcgaatgc gaatagtgca tcgaatggga caaaatcatt gtccttatct
     3181 ggattggcgt cttcaacgat gtatacggtg tgggtgaatg caactgatcc tgctgggagt
     3241 ggtctgtgga ctcgtcaatg gtttacgttt attacgcaag gaattggtgg gaatagccca
     3301 ccagagtttg ggaccccctc cccagcgaac ggctcttccg ggaaccggct gtatttcaat
     3361 tggaatattc cggttaatga tcctgaggga accgactttt cctggaccat tcaatgcaac
     3421 aacggacaaa ccaccagtgg aacgagcgca aacaatggaa caaaatcact gtttctatct
     3481 ggacttgcct attcaactag ctacaaaatc tgggtgaatg caacagatcc aaccggcagt
     3541 actcaataca cacgaaaatg gtacacattc accacagaaa ccattattcc ctttaatatt
     3601 ccaacaccaa actcatgggt ctttgtcacg tcagcatctg gagaatcaag agtacaaccg
     3661 tctgttgctg atatcaataa cgatggacga atggaaatca tacgatcagg aataaacgga
     3721 atcgtagttt atgacggaaa aacaggcact attctttggg ataacataac aacgatgtgg
     3781 gatagtcata atccactaga aatcattgat ctcaacaatg atggttacct tgatgtgatt
     3841 agctcatata acacaggaac cagggctctc tctggagaag atggatctca attatggtat
     3901 aatgcaaatg cacccttata taataaacat gcagtcgctg gtgatatcaa cgcagatgga
     3961 taccctgaag tctttgtctg tgcagcaggg gccgaagatg gaacacccga aggatggata
     4021 aaaggcctta cgcatgatgg aacaattttt gcccaagtac atgtgtactt cccctgttat
     4081 gggggattat cactggggga caccgatttt gatggtgtgt atgaagtcta tctctgtgaa
     4141 agaaacatag ggtatgacgg aaatgtggta ggcaaaggag tcgcagcgtt ttgggcgtca
     4201 aatctcacac aacgatggag tcacccagag atgttatcaa gcagccattg cccaacactt
     4261 gtggacacga acaaagatgg aatactcgag gttgtcgctc tcgctcaaac agggagtaac
     4321 ggcattgctg tctacaacag ttctgatggt actgttatcc accaatcaaa tatccccgga
     4381 gtgagatgtc attcgcaacc aacagtgtat gatatcgatg gagacggaaa tctggaaata
     4441 atcgtaggtg gtggcagtga tgcttgggga aaaccagcga tctgggatct gtatacctgg
     4501 tctcttgaag cgtggcttcc attcaattgt tgggaacctc ctgcaatcgt tgatctgaat
     4561 ggggatggac gtgttgagat tcttgaatgt accataacaa atatctcaat cttcgatgat
     4621 aattatgtgt tcagaggatc catcccgctc gataataata gaagtggcta tggttggtat
     4681 ggtatgtcaa tgatcatagc acaagatatt gataatgata ataaattaga attagtgata
     4741 aatcgaaata cccggacgta tgcctatgat accgatggag ctgcaccaac cccactagca
     4801 tcatctcaat acatgtacta cagtccactc cgcggccgat acccctattc aacaccatat
     4861 ggggcaacgg caccttgggc aataaatgaa tatcccgcta atgaaagtac gaatattccc
     4921 tttaaccctc aactttcagt gtatatctat gataatcaag aagattcaat gaatatcatc
     4981 tttagaacca atgcatccac aggtgtatgg cataacattg aaacctattc aaatatacat
     5041 gaaggaactt acacagccga taccaccgat atggatactt ctctaaccta ttattggtgg
     5101 agcattacag taacagattc aacagcaaaa acaacatata aattatataa atttaagacc
     5161 atcaacggtg aacctgagcc gccatatact cctagtaacc cattccctgc aaatgaatct
     5221 acaggtgttc cagtgactgc tgatcttggt tggactggtg gcgatcctaa tggcgaccct
     5281 gtcacctatg atgtctactt tgggacgacc aatcctcctg caaaaaaagt atacaatcaa
     5341 tcagctcttc tctataatcc aagcacgatg agcaacatca cccattatta ctggaagatt
     5401 atcgcatggg acaatcacag cacctcaaca gaaggaccac tctgggaatt taccacagga
     5461 ctaaaagtga acaacccacc taacccgcca agcaacccat cccctgcaaa tggcgcgacc
     5521 aaggttgtta ttagcgctaa actgagctgg actgggggag accctgatgg tgataccgtg
     5581 acctatgatg tctactttgg aacaacaagc caacctccaa aagtcaatac taatcaatca
     5641 gctcttacct ataatcctgg agcattagcc tatgacacga cgtattactg gaacattatc
     5701 gcttgggata accacagtgc aaacgtatca ggcacaactt ttgacccctg gaacttcacg
     5761 acaaaaagcc ccggcggtgg cgggggt
//
LOCUS       DATMXJ010000153         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_45154, whole genome shotgun sequence.
ACCESSION   DATMXJ010000153 DATMXJ010000000
VERSION     DATMXJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45154"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1142)
                     /locus_tag="VN377_04060"
     CDS             complement(108..1142)
                     /locus_tag="VN377_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HWR27597.1"
                     /translation="MESWKTYLHADPTAWLLEPNNPSVRFVTLCDLLEKPETNKDVKE
                     AKQDIMKTGVVPAILAKQKNEGYWEDPERFYSAKYKGTVWQLMILAELWADGNDDRIR
                     NACEFILTHSQDPESGGFAYSTSMKKGGGRHSEVIPCLTGNMIFSLIRLGYLNDSRIQ
                     HGIEWITTYQRYDDKIAEVPKGWPYDRLKTGCFSRHSCHMGAVKTLKALAEIPENKRN
                     TAVKKTIEKGAEYFLLHHVFKSSHHLDRVPKPGWLKFGFPLMYQTDVLEILGILTKLG
                     YEDQRMQEAVDILVSKQNTDGRWNLENTFNGRYQVNIEKKEEPSKWITVRALQALKRY
                     YSCDWEYCVV"
     gene            complement(1168..1890)
                     /locus_tag="VN377_04065"
                     /pseudo
     CDS             complement(1168..1890)
                     /locus_tag="VN377_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          572 a          489 c          416 g          570 t
ORIGIN      
        1 cctttagagg gcaggcgaat gcctgccgcg tgcgacaaaa aagagatata ctttagctgg
       61 agaaaaatag agatgcgact tttcatctgc gggtctttca cttcgtttca gaccacgcag
      121 tattcccagt cgcaactata atatcttttt aacgcttgta atgcacgtac tgtaatccac
      181 ttgcttggtt cttctttctt ttcgatgttc acctgatagc gtccattaaa tgtgttctcc
      241 agattccacc tgccgtcggt gttttgtttt gaaaccaaaa tatcgacagc ctcctgcatc
      301 cgctgatcct catatcccag ttttgttaaa atccccagga tttccaggac atcagtctga
      361 tacatgaggg gaaaaccaaa tttcagccac cctggtttcg gtacacgatc caggtgatga
      421 ctgcttttaa acacatgatg taacaaaaag tactcagctc ctttttcaat cgtctttttc
      481 acagcagtgt tccgtttgtt ttcagggatt tcagctaagg ctttgagtgt ttttactgca
      541 cccatatgac acgaatgtct gctgaaacat cctgtcttta atcgatcgta tggccaacct
      601 tttggcactt ctgctatctt gtcgtcatat cgctgatagg tggtgatcca ttcaatccca
      661 tgttgaatcc gagaatcatt cagatagcca agtcgtatta aactgaatat catattccct
      721 gttaaacaag ggattacttc actatggcgt cctccacctt ttttcatgct ggtggaatac
      781 gcaaaccctc cgctttccgg atcctgcgaa tgagtaagaa taaactcgca tgcgtttcgt
      841 atccgatcat cgtttccatc agcccaaagt tcagcaagaa tcatgagctg ccagacagtg
      901 cctttgtatt tcgctgaata aaatcgctca gggtcctccc aatatccttc gttcttttgt
      961 tttgctaaaa tcgctggtac aacaccggtc ttcatgatat cctgttttgc ttcttttacg
     1021 tccttatttg tttctggttt ttctaacagg tcgcagaggg taacaaatcg cactgaagga
     1081 ttgtttggtt caagaagcca ggctgtgggg tcggcatgaa gatatgtttt ccaggattcc
     1141 atgtgaatat accttttgat tatttctcta aattttcatt ttcttaaacg cataggtccc
     1201 aagaaggatc atgataagag caaagaggca gactaacgca aaatcgaaca acaggggaaa
     1261 ttgagacatt cctaacaata caccgcggat tccatctacc gcatacgtca ctggatttac
     1321 aaaaatgaat ggtgcaagcc atgctggaag attggtgatc gggaacagag cacctgaaag
     1381 gaagaataac ggaaaaataa ggaaactgat gatcaattga aatccttcag ggctttccat
     1441 ctgtgaacca aggataaggc cgacgctgac gagccctaca gtcgccacca ttaaaaaagc
     1501 aagcgaaaca gcaacagaaa aaacagtgaa atgcaaccct ggcatgatcc cgctgacagt
     1561 aaaaacaatc ccgatcacaa ataaaatcag gatttgaagg agggtgtcgg tcgacccgcc
     1621 aacgattttt ccaatgaaca gcgaggtccg tctcatcggt gcgaccaaga cttctttcat
     1681 gaaatctact tttttatccc agacgatgta cacaccaaag aagacagagg aaaacaacgc
     1741 agtctgaata aggactccgg ggtaaataaa cgtctgataa ttcaccccgc taaacgaaac
     1801 cgctgaccct agaccgcctc cgatgatcaa caaccagagc aaggggttga taatcgatga
     1861 gacgacccgt gatcgttctc gcaggaagac ttttgtctcc ctgtatcaca aggcatagat
     1921 tcctttgagt tcgctcattt tttctcactc gcattcatca ttttctcagc ccaactaccc
     1981 tcaggagtac cttcgcgtat cgctctgccg gtataatgca ggaatacgtc gtcaagggtc
     2041 ggtgacc
//
LOCUS       DATMXJ010000154         5898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_70408, whole genome shotgun sequence.
ACCESSION   DATMXJ010000154 DATMXJ010000000
VERSION     DATMXJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70408"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VN377_04070"
     CDS             complement(<1..405)
                     /locus_tag="VN377_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWR27598.1"
                     /translation="MKLFIDTADLNEIKEIASWGILDGVTTNPTLLAKCGRSLNEIID
                     EIFELVDGPISLEVMSEKADDMVTEAKQIVSKIPQKHRNNVAIKIPMTSEGLKAVKTL
                     CKEGIKTNVTLIFSANQALLAAKAGATFVSPFI"
     gene            581..736
                     /locus_tag="VN377_04075"
     CDS             581..736
                     /locus_tag="VN377_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR27599.1"
                     /translation="MPLTRKARIVGSSLVITIPSQLAKAHDINDGDELEIIPAGIGEF
                     KIRKLKK"
     gene            854..1141
                     /locus_tag="VN377_04080"
     CDS             854..1141
                     /locus_tag="VN377_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010901613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HWR27600.1"
                     /translation="MNEEIIKLERGQVTGYYIDLGKAPLLILQAKKGYIMCGYLNMNT
                     ANKLGDVAGKVTGVKTFDDMLKATVIEVSENAKKEGLKEGMSAQDFLNNLL"
     gene            1151..2359
                     /gene="ilvA"
                     /locus_tag="VN377_04085"
     CDS             1151..2359
                     /gene="ilvA"
                     /locus_tag="VN377_04085"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWR27601.1"
                     /translation="MVTFKDVKEAYQILQPVIRKTPLEKSKTFSNLVKANVYLKEENL
                     QTTGSFKIRGAYNKIYHLTEKEKKQGVVCASAGNHAQGVAHASTLMGVNSTVYMPIFT
                     SPAKINATKSYGAQVVLEGVTYDDAAEAAKKYAEEKKMTFVHAFNDEYVIAGQGTIGI
                     EIFEELPTVDAVIIPIGGGGLVSGIALTLKTLKPKVKIIGVEAEGAQSMKLSLEQHKI
                     IPLKSIMTIADGIAVKTPKELTFALAERYVDEVVTVNDEEIANALYLLLQRTKLVAEP
                     AGAVALAALLSKKISFPKKNIVAVLSGGNVNLSLLTQIIERGMMRHKLLVKVSITALD
                     KPRVLKDILTILANVGSNVQSIEHDRCTTAVPVGYVKIVITFQTLGKEQIEMIKDKFD
                     QKKVQYHILS"
     gene            complement(2382..2705)
                     /locus_tag="VN377_04090"
     CDS             complement(2382..2705)
                     /locus_tag="VN377_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27602.1"
                     /translation="MRNILTDQPANASLPSDKKAESKSPMENTSCSEQIIKGLKIEST
                     LFCLDTGKEKYLFATEDEAIEQLKKLEKSSINPDNSQIVQVTTTGNAWKLTQVPWSHI
                     AIKLL"
     gene            2843..4081
                     /locus_tag="VN377_04095"
     CDS             2843..4081
                     /locus_tag="VN377_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR27603.1"
                     /translation="MHDRFYLRLLSSLYISTFFIRASFGIMLATLPIYLNITSEYIGY
                     GIVSAASPLFEMGTVLFIGAVVDRYGRKIVLLSGLLTAAFLLFLLSITKNLYGIFCIN
                     AAHGMAAGAILVSSLALLTDYAPEHRRGREMGAFDAFNMLGWIVGYAVGALFLEFFKG
                     SLGHTFLIAGVMALVGCVYCFVNITEPKKSEVICQKIRFNTIVSVFKQRSVILLTLPW
                     FIVFMIIGAVLTFLTVSTAQGGILTISPILLSGIICGVGIVLITTQVIFGRLSDKYGR
                     LPIMLVGTIGFVGVMTVVGIGYGIAPTSNGEDVKNSILQLWPLLGIFGFMALAFGPSA
                     LSSLADEAQETKRGVTMAVYSIVISAGMFVGVPAVAAIFDRYGGPGVLFFMIGCAIAM
                     LCLMIARYVQLKHQKKQTMV"
     gene            complement(4125..5288)
                     /locus_tag="VN377_04100"
     CDS             complement(4125..5288)
                     /locus_tag="VN377_04100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWR27604.1"
                     /translation="MLDFNYKVSTQIFFGRNSIGHLGKELKQYGTRILFVYGQGTIKK
                     TGLYDSVTDIFLRNNIIFQELSGVQPNPRISQVRQGIKLCREHDIACIVAVGGGSVID
                     CAKTIATGFYHDGDPWDLFLYGDSSVKKALPIGTVLTCTGTGSEMNGNAVISNEETVE
                     KLAIHHDILRPRFSILDPTYTFSVPSNQTAAGTVDIFSHILEQYFSPTKDAFVQNRIA
                     EGLLKTCIKYGPVAVAEPTNYEARAQLMWTSSIALNGLLGYGRISDWATHGIEHALSA
                     VYDVTHGVGLAILTPHWMEYVLNDQTRDKFVEYARNVWQIEGNDDVVIGMKGIEKTRE
                     FFTHLGMPQTLRDVGVKQNTLTKMAEKTVMHGDVGKFRKLGKNDVLTILKNAF"
     gene            complement(5365..>5898)
                     /locus_tag="VN377_04105"
     CDS             complement(5365..>5898)
                     /locus_tag="VN377_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27605.1"
                     /translation="MMPVMGVALIIVSTGIDRGIFGDPGGFLANQMRTATLFLIFTSC
                     LISPILLKRSMGSPLGKKFGKTKTKLSSYHHPHCAECFSALRLDPANNKWYCDGCQGY
                     VEIRKKTPRHTLGKTDRNIKYIIGAGTILLCGYVIQSSIRMTVFEKITAIIGIFLGTT
                     LAFFALKLLFSPQKTTS"
BASE COUNT         1712 a         1277 c         1238 g         1671 t
ORIGIN      
        1 gatgaacggg ctgacaaagg tcgcaccagc ctttgctgct agaagagctt ggttcgctga
       61 aaaaattaat gtgacgtttg ttttaatgcc ttctttgcag agtgttttga ccgctttgag
      121 cccttcagag gtcattggta ttttaatggc gacattgttt cgatgcttct gcggtatttt
      181 tgagactatc tgctttgcct ctgtgaccat gtcatctgct ttttcactca tcacctcaag
      241 gctgatcggt ccatccacaa gctcgaagat ttcatcgatg atctcgttca atgatcgtcc
      301 acatttcgcc agcaaagtcg ggttggtggt cactccatcc agtatccccc atgatgcgat
      361 ttccttaatc tcatttaaat ctgcagtatc gataaatagt ttcataatgc ctcacgccat
      421 cctccggtaa ctggtaataa ttgataatgt tttggttttt acccgagggt ttgaacggag
      481 ggaattttcg aaaaaagaga tatgcatcta gatatatttg aaaatttaga aacatatata
      541 tatctagcta tacattacct actcttgcag gaggggattc atgcctctaa caaggaaggc
      601 acgtatagtg ggaagcagcc tcgttatcac aataccgagt cagttagcaa aagcacatga
      661 tatcaatgac ggggacgaat tagagataat tccagcggga atcggggaat tcaagatacg
      721 gaaactgaaa aaataaacga cctgtcctat ccatgatcta ctactcctgg gatttacaaa
      781 aaaccttttc ttcaatgagt tcatgaataa ataaatccaa aacaaaacgg ggaattaaac
      841 agctgaggtc atcatgaatg aagagataat aaaacttgaa agagggcagg tcacaggcta
      901 ttacattgat ctaggaaaag ctcccttgct catcctccaa gcaaagaaag gatacatcat
      961 gtgcgggtac ctgaatatga atactgcgaa taaacttgga gatgtcgcag ggaaagtcac
     1021 cggtgtgaag acctttgatg acatgctcaa agcaaccgtc atcgaagtat cagaaaacgc
     1081 aaaaaaagaa gggcttaaag aaggaatgag cgcacaggat ttcctaaaca acctcctctg
     1141 aggaacacac atggtcactt tcaaagatgt caaagaggca tatcaaatcc tgcaacccgt
     1201 catcagaaag acaccattgg agaaatcaaa aacattttcc aacttagtaa aagcaaacgt
     1261 gtacctcaaa gaggaaaacc tccagacaac aggatctttc aaaataagag gcgcgtacaa
     1321 taaaatctat catttaacag agaaggagaa aaaacaaggc gtcgtctgtg cctcggcagg
     1381 aaatcatgca cagggcgtag cacatgcctc aaccttaatg ggcgtgaaca gtaccgtcta
     1441 catgccaatt ttcacatccc cagcaaaaat taatgcgaca aaatcttatg gggcacaggt
     1501 tgttctcgaa ggagtcacct atgatgacgc ggcagaggca gcaaaaaaat acgcagaaga
     1561 aaaaaagatg acattcgtcc atgctttcaa cgatgagtac gtcatcgcag gacaaggaac
     1621 aatcggaata gagattttcg aagaactccc aacagtagat gccgttatta tccccatcgg
     1681 tggcggaggg cttgtgagcg ggatcgctct taccctaaag acactgaaac caaaagtaaa
     1741 aatcatcggt gtcgaagcgg aaggcgcaca atccatgaaa ctgtcattag aacaacacaa
     1801 aataatacct ttgaaaagta ttatgaccat cgcagatgga atagcggtaa aaacaccaaa
     1861 agaattgacg tttgctctcg cagaacggta cgttgatgaa gtcgtgacgg taaatgacga
     1921 ggaaatcgca aacgcccttt acctattgtt acaaagaaca aaactcgtcg cagaaccagc
     1981 cggcgcggtc gcattagctg ccctgttaag caaaaaaatc tcctttccca aaaaaaatat
     2041 cgtcgcagtc ctcagcgggg gaaatgtcaa cctttcctta ctcactcaga tcatcgaacg
     2101 agggatgatg cgtcataaac tcctagtaaa agtatcgata acagcccttg ataaaccaag
     2161 agtactgaaa gatattctga caatacttgc gaatgtcggt tcaaatgtac aatcaataga
     2221 acatgaccga tgcacgacag cggtacctgt cgggtatgtg aaaatcgtaa tcacattcca
     2281 gacattagga aaagaacaaa ttgagatgat aaaagataag ttcgaccaga aaaaagtaca
     2341 gtaccatatc ctcagctaaa aaaaaaggat gaaaattgtt ctcacagaag ttttatagca
     2401 atatgtgacc aaggcacctg ggtcaatttc caggcgtttc ccgttgtggt cacctggaca
     2461 atttgtgaat tatccgggtt aatgctgctt ttctcgagtt ttttaagttg ttcaatcgcc
     2521 tcatcctcag tcgcaaaaag atatttttct ttccctgtgt ccagacaaaa cagcgtgctt
     2581 tctatcttca gacctttgat gatttgttca gaacacgatg tattttccat aggtgatttc
     2641 gattccgctt ttttatctga tggcaacgag gcattagcag gttgatctgt taaaatattt
     2701 ctcatgacat ccacctcaca cgaatgatac attcacctcg aggtataaat atttatggcc
     2761 tctaattttt atgattttga tgatttgatt ctcaatagaa gaacctattt atagcgatac
     2821 ttttttactt ggtctgacca ctatgcatga ccgattctac cttcgattac tatcatctct
     2881 ctatatctca acgtttttta tcagagccag ctttgggatc atgctcgcca cacttccgat
     2941 ttatctgaac atcacttcag agtacatcgg gtatggtatt gtatctgctg catctccgct
     3001 cttcgagatg gggaccgtct tatttattgg cgcagtcgtc gatcgttatg ggagaaaaat
     3061 cgtattatta tccggtcttc ttactgcagc gttccttctg tttcttctct cgatcacaaa
     3121 gaacctctat gggatctttt gtatcaatgc agctcatggg atggctgcag gtgccattct
     3181 tgtttcatca ttagcactgt taacagacta cgctcctgag catagacgag gacgggaaat
     3241 gggtgcattt gacgcattca atatgctcgg atggatcgtt ggctacgccg tgggagccct
     3301 tttccttgag tttttcaaag gttctctcgg gcatacgttc ctaatagctg gtgtgatggc
     3361 gctagttggt tgtgtgtact gttttgtaaa tatcacggaa ccaaagaaat ccgaggtgat
     3421 ttgccagaaa ataaggttca atacgattgt cagtgtgttt aaacaacgtt ctgttatcct
     3481 gcttacgctt ccatggttca ttgtgtttat gatcatcggg gcggttctga cgttcttgac
     3541 cgtctcaacc gctcaaggtg gaattcttac gatttcacca atccttctct ctggcatcat
     3601 ctgtggtgta ggaatagtac tgatcacaac tcaggttatc tttgggcgtc tttctgataa
     3661 gtatggacgt ctcccaatta tgctcgttgg tacgattgga tttgtcggtg tgatgacagt
     3721 cgttggtatt ggatacggca tcgctccaac atcgaatgga gaggacgtga aaaacagcat
     3781 tcttcaactc tggccgcttc tcgggatttt cgggttcatg gctcttgcgt ttgggccctc
     3841 ggcgttgtcg tcacttgcgg atgaggcaca ggagacaaaa cgtggggtga ccatggctgt
     3901 ttatagtatt gtcatcagcg cagggatgtt tgttggggtg cctgctgtcg ccgcaatctt
     3961 tgatcgatac ggtggacctg gtgtattgtt ctttatgatc gggtgcgcga tagcgatgtt
     4021 atgcctcatg atcgctcgct atgttcaact gaaacatcag aaaaaacaga caatggtatg
     4081 acaagaaaat cagaaaaaaa catcaatctt gttttttgtc tatgctagaa agcattttta
     4141 agaatagtga ggacatcgtt ttttcctagt tttctgaatt tcccaacatc accatgcatg
     4201 actgtttttt cggccatttt cgtaagagtg ttttgcttca caccgacatc ccggagtgtt
     4261 tgtggcatgc caaggtgggt aaaaaattcc cttgtttttt ctatcccctt cattccgatg
     4321 actacatcat cattaccttc tatctgccat acattccgag catattcaac aaatttatct
     4381 ctcgtttgat cattgaggac atactccatc caatggggtg ttaaaatcgc taaaccaacc
     4441 ccatgggtca catcatagac agcacttaat gcatgttcaa tcccatgtgt cgcccaatca
     4501 ctgattcgcc cgtaaccaag aagaccattg agtgcgatgc tactggtcca catcaactgc
     4561 gctcgcgcct cataattcgt aggctctgct accgctacag gaccatattt aatacaggtc
     4621 ttcaggagac cctccgctat tctgttttgc acaaacgcat ccttggtagg actgaaatat
     4681 tgttcaagga tatgactaaa aatatcgaca gttcctgctg ctgtttggtt cgaaggtaca
     4741 gagaacgtat aggttggatc aaggattgaa aaccttggtc gtagaatgtc atggtggata
     4801 gcgagttttt ctacggtttc ctcatttgag attacggcat tcccgttcat ctcagaacca
     4861 gttccagtac atgtcaggac cgttccaatt gggagcgctt ttttcaccga agaatctcca
     4921 tagagaaaca gatcccaggg gtctccatca tgatagaagc cggtcgcaat cgtctttgca
     4981 caatcaatga cactgccacc accaacagca acaatacatg caatgtcatg ttccctgcaa
     5041 agcttgatac cctgccgcac ctgagaaatc cgtgggtttg gttgcactcc ggaaagctcc
     5101 tgaaagatga tgttatttct caggaaaata tctgtaacag aatcatataa ccctgttttt
     5161 tttatagtcc cttgaccata gacaaacaga attctcgtcc cgtattgctt caactctttg
     5221 ccgagatgtc cgattgaatt tctcccaaaa aaaatttgtg ttgaaacctt ataattaaaa
     5281 tcaagcatag gttcgcctct gtttctatcg tcaatgagtt tccaaatata aaaattagga
     5341 accgaatgtg gatatcacag aaagttagct agtggttttt tgaggtgaaa aaagaagttt
     5401 aagcgcaaag aacgcaagag ttgtcccaag aaagatcccg ataatcgccg ttattttctc
     5461 aaatactgtc atacgtatag aactttggat cacataccca cataagagaa tcgtgccagc
     5521 gcctatgatg tatttgatat tcctatctgt ttttcctaac gtatgccgag gagtcttttt
     5581 tcgtatctcc acataaccct gacagccatc acagtaccat ttgttgttcg caggatctaa
     5641 tcgtaaggct gaaaaacact ctgcacagtg aggatgatga tatgatgaaa gcttcgtttt
     5701 tgtttttcca aattttttcc ctaacgggga tcccatactc cgtttgagta agattggact
     5761 gataagacaa ctggtgaaga tgaggaagag ggttgcggtt ctcatttgat tcgcaagaaa
     5821 accaccaggg tcgccaaata tacctctatc tatcccagtc gagacaataa tcaaggcgac
     5881 tcccatcact ggcatcat
//
LOCUS       DATMXJ010000155         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28582, whole genome shotgun sequence.
ACCESSION   DATMXJ010000155 DATMXJ010000000
VERSION     DATMXJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28582"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VN377_04110"
     CDS             complement(<1..1031)
                     /locus_tag="VN377_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27606.1"
                     /translation="MNTLVKLMITIAVTVILVCSGYALLLNGQTSQPDTSKDTSQDSN
                     ESNDTVAPTITSVTGDLVVDAGTTITITTTFSDDVAVTSAKLFYKPATAIAWSSKSIL
                     TGSATFPLPAGSNESWYYYITVDDVAGNGPIGDPSADGSSFYTITVLDTPTNPPDDND
                     TNGDGGNTSQNSSRFVFIELGTKIVCSECPKISVILNELYNSGDYPFYYVSLPQDNPD
                     ALARINDYNIWGYPTVYIDGGYTTLIGSSIQKSDFEKNVTSAAERITPRIFIRTTAHW
                     DNRTNIIAVNVKVENQETSEYQGRLRVYITELISTKWQAGTPMHFSFLNFLINEDVQI
                     PANDHRIIN"
     gene            complement(1154..2443)
                     /locus_tag="VN377_04115"
     CDS             complement(1154..2443)
                     /locus_tag="VN377_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27607.1"
                     /translation="MRILITDEMSLSTIFTSFDRSFLYSFSFVPTVITARQLAAVTPA
                     SHSLTIQEGNTTIDFEADYDVVHINFKTALAPRAYEVADIFRKKGKRVVLSGYHPSAL
                     PAEAAQHADSVIVGDAVTLWPAVVKDLEAGTLQPFYTSADTHDTFILPSTQAIKLKGL
                     QLTNSIEATRGCPHKCDFCQDANIRDGGLFRTRSLDDVIEEIASLQQKIFFFTDFSLT
                     IDLAYTKELFRRMHRLRKKFICGGNVDVLAEDEELLRLSHDAGCIEWISGFETFSQES
                     LNGAHKKTNIVEDYTRAVKKIHKYKMAVFGTFVLGFDEDTPDVFQVMRTHIGRLGIDA
                     VNFALLTPYPGTPLFNRLEKEGRILTKDWSKYNRKNVVFEPKNMTKKQLEEGFRNITL
                     SFSSLPSMGYRTMRSLNLGLYPFLATFAGNLGQFMKT"
     gene            complement(2511..3152)
                     /locus_tag="VN377_04120"
     CDS             complement(2511..3152)
                     /locus_tag="VN377_04120"
                     /inference="COORDINATES: protein motif:HMM:NF023242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HWR27608.1"
                     /translation="MNSITRNLPLLDVPYFQQTRTATCGPACLMMVMKYWNPSLEFSR
                     KFEVQLWMKSYSLFLFGGTFQYGLAAAAGTLGFKTEIYQKLRFSDGYPRLPRFASLIE
                     NIVSRKSRRMHIPIVYRQENLRVIADALNQSIPPIVFINLHPVLGENVFHWVVVTGVD
                     DQNVYINDPYVPRELAGQQKKNYPLPLDVFTQAMMTEIGRNLRFPPCVVLVHP"
     gene            3476..>4053
                     /locus_tag="VN377_04125"
     CDS             3476..>4053
                     /locus_tag="VN377_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR27609.1"
                     /translation="MKTYDVIVVGSGSGGEIVDAAVSHGLTVAWVDKGPLGGTCLNVG
                     CIPSKILIHPADRIMEILEAKKLGITAEIKDIDFQAIMQRVQRPIQESHEATEHGISH
                     AENLDYYKNECFFIKEYTLEVSGKDIKGEKIFLGSGARPLIPPIKGLDAIPYLTNENV
                     FSLKEKPKDMVIIGGGYIAVEFAHFFSAVGTNV"
BASE COUNT         1205 a          836 c          901 g         1111 t
ORIGIN      
        1 ttattgatga tacgatggtc attggcaggg atttgaacat cctcgtttat cagaaaatta
       61 aggaaggaga aatgcatagg ggttccagct tgccattttg tcgaaatcaa ttcagtgatg
      121 tagactcgta accgcccctg atactccgag gtctcctgat tttctacctt cacgtttaca
      181 gcgatgatat ttgttcggtt gtcccaatgg gcggtcgtgc gaatgaagat acgaggagtg
      241 attcgttctg ctgcggatgt gacgtttttt tcaaaatcag atttttggat gctgctaccg
      301 ataagggtcg tataccctcc atcgatatag accgttggat atccccagat gttatagtcg
      361 ttgatgcggg ctaaagcgtc ggggttgtct tgcgggagac taacgtagta gaatggatag
      421 tcccccgagt tgtataactc attgaggatg actgatatct tagggcattc tgagcagacg
      481 atcttggtgc caagttcaat gaatacaaaa cgtgaggaat tctgcgaagt attccctcca
      541 tcgccatttg tatcattgtc atctggagga ttcgttggcg tatctaggac cgtgatcgta
      601 taaaaggaac tcccatctgc cgaaggatca ccaatcggtc cattccctgc aacatcatca
      661 acagtgatat aataatacca gctctcattt gatccagcag gaaggggaaa agtcgctgac
      721 ccagtaagaa tcgacttgga gctccatgca atcgcagtgg ctggtttata aaataattta
      781 gctgaggtca ccgcaacatc atcagaaaag gtcgtcgtta ttgttatcgt agttcctgca
      841 tcaacaacca gatcgccggt gacagaggta atagttggag caaccgtgtc gtttgattca
      901 tttgaatctt gactagtatc ttttgatgta tcaggttgag atgtctgtcc atttaatagt
      961 aatgcatatc ctgagcaaac aaggataacg gttacagcaa ttgtgatcat gagtttaacg
     1021 agggtattca tgtcactact attatgagtc gacgagatat ataaatttaa cgattgttaa
     1081 gttttttatc attttacaca aaaattccaa aaaatcaaaa aaaagtgaaa ggaataaagg
     1141 atatccattc ctgttaggtt ttcataaatt ggccaaggtt cccagcaaag gttgcaagaa
     1201 aagggtatag tccaaggttc agacttcgca tcgtcctgta ccccattgac ggaagagaac
     1261 taaaagataa cgtaatgttc cggaatcctt cctccagttg tttttttgtc atgttttttg
     1321 gttcaaaaac gacattctta cgattatatt ttgaccaatc ctttgtcaat attcgccctt
     1381 ctttttccaa ccggttgaac aacggagtcc cgggataggg ggtgaggagt gcgaaattca
     1441 ctgcatcgat accaagtcta ccgatatgcg tgcgcatcac ctgaaaaaca tcaggggtat
     1501 cttcatcaaa accaagaaca aacgtcccaa acacagccat tttgtattta tggattttct
     1561 ttactgcacg agtatagtct tccacgatgt tagtcttttt atgcgcacca ttgagtgatt
     1621 cttgggaaaa cgtctcaaac ccgctgatcc actcgatgca tcctgcatca tgagaaagac
     1681 gcaacagctc ttcatcctca gccaagacat cgacgttccc cccacagata aatttcttcc
     1741 gtaatctgtg catacgacga aacagttctt tcgtataggc caaatcgatg gtgagtgaaa
     1801 aatcagtgaa gaaaaatatt ttctgctgaa gagatgctat ttcttcaatg acatcatcga
     1861 gagaccgggt gcgaaataat cctccatcac gaatatttgc gtcctgacaa aaatcacatt
     1921 tatgaggaca tcctcgtgtc gcttcgatgg aattcgtcag ttgaagacct tttaatttta
     1981 ttgcttgcgt cgatggcaag atgaatgtat catgtgtatc agctgaggtg tagaatggct
     2041 gtagggtgcc tgcttcaagg tccttgacca cggccggcca aagggtaacc gcatccccca
     2101 caatgacgct atccgcatgc tgtgctgctt ccgcaggtaa cgcagaagga tgatacccac
     2161 ttaaaacaac tcgtttccct tttttccgga agatgtcagc aacctcgtac gcccttggag
     2221 cgagagcggt cttgaaattg atatgcacca catcgtaatc cgcctcgaaa tcaatcgtcg
     2281 tattcccttc ctgaatggtc agcgaatgag atgccggggt gaccgctgct aactgtcgtg
     2341 ccgtaatcac cgtaggaaca aaggagaacg aataaagaaa tgagcgatca aaagaggtaa
     2401 atatagtact gaggctcatt tcatcggtaa taagtatccg catgcggcat taaaaaaaag
     2461 gttaaggaca tttaaaggtt cgcgtcaacc atatgatcat gaaaaaaaat ctagggatga
     2521 actaaaacaa cgcatggagg aaaccggagg tttctgccaa tctcagtcat catggcctga
     2581 gtaaagacat ccaaggggag agggtaattt ttcttttgct gtccggccaa ctctcttgga
     2641 acatatgggt cgttgatgta cacattctga tcgtctacac cagtgacgac gacccaatgg
     2701 aagacgttct caccaaggac aggatgtaaa tttataaaaa caataggtgg gatcgactga
     2761 ttgagtgcat cagcgattac cctgagattc tcttgtctgt aaacaatcgg tatatgcatg
     2821 cgacgggatt ttcgtgaaac gatgttttca atgaggctag cgaaccgggg taatctcgga
     2881 taaccatctg agaaccgcag cttttgataa atctcggttt taaaaccaag cgttcctgca
     2941 gctgctgcca atccatattg aaatgttcct ccgaacaaga acaacgaata gcttttcatc
     3001 cagagttgaa cttcgaattt tcgtgaaaac tccaatgatg gattccaata tttcatcacc
     3061 atcattagac acgcaggacc acaggtggca gttcgcgtct gctggaaata cggaacatcg
     3121 aggagaggaa gatttctggt gatgctattc atagatactg atatctgcgg tgttttaaaa
     3181 aacactcgga agaaagaatc tcggatctgg acggtgaaac ataatgatgg aaaacaaatt
     3241 aaaaacacag cgatgaatat aaaaatcgtg ataccttgga gattctacct aattttttct
     3301 cgttttgtca agatgacaag gaatccctat catatgagtg aatttacgta tacaaacctt
     3361 tcaatctaaa tcgaaaacag gtaaccacat tcatatatat ctgaacgtta ttcaattcat
     3421 tgcatcgaag tcagttcttc taaataaata atgaaaatat gatgaagagg taaggatgaa
     3481 aacatatgat gtgatcgtcg tcggaagcgg ttctggcggg gagattgtcg atgccgcagt
     3541 atcccatggg ttgaccgtgg catgggtcga taaaggacca ctcggaggaa cctgtctgaa
     3601 tgtcggctgt atcccttcaa aaatactgat ccatcctgct gatcgtatca tggagatttt
     3661 agaagcaaaa aaactgggaa tcaccgctga aataaaggac attgatttcc aagcgatcat
     3721 gcagcgggtt caacgaccga tccaagagag ccatgaggcg acagagcacg gtatttccca
     3781 tgcagagaac cttgattatt acaaaaacga atgttttttc atcaaagagt acacactgga
     3841 ggtttctggg aaggacatca aaggagaaaa aatctttctt ggctctggcg cacgacctct
     3901 tatccctccg ataaagggac tcgatgctat tccctatttg acaaatgaaa atgttttttc
     3961 tttaaaagaa aaaccgaagg acatggtgat catcggtgga ggatatatcg ctgtggagtt
     4021 cgctcatttt ttctcagcag tagggaccaa cgt
//
LOCUS       DATMXJ010000156         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55506, whole genome shotgun sequence.
ACCESSION   DATMXJ010000156 DATMXJ010000000
VERSION     DATMXJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55506"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..1411)
                     /locus_tag="VN377_04130"
     CDS             complement(419..1411)
                     /locus_tag="VN377_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27610.1"
                     /translation="MKKQLVIIGILVVLLAVGFSGCETKQNGNQLDINQDGNQNNGAQ
                     NETELAGKIVFISTGNIYVVNLDGSDLIQLTDFSLGAQDPVWSPDGKMIAFSATNDGG
                     IYTINADGTSLTRIVYKTVYDAYTDTWNVAANNHPSWSPDGSAIVYESLGDENSGTTV
                     SNANIYVVNSDGTGMRSVTNEPSYEGQPSWSPDGNKIAFVYVKAEGDGYSNYHIYEMN
                     ADGSNWVQLTNRSITVNNQNPDWSPDGTKIVFTGDGDEHQGVFCIKLVDKSLTFVGAL
                     GGKPTWSPGGSEIMTYGYESGTGSMRIWVTNADGSNSKKVDISIDAYEPDWTSQ"
     gene            1798..>2977
                     /locus_tag="VN377_04135"
     CDS             1798..>2977
                     /locus_tag="VN377_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWR27611.1"
                     /translation="MLRPHYTQYAKTLLFLSILLFITSFLFTTLLPAFTAMLLIVFMV
                     YTKTHFKPNPDQLIIQRRIMEPLVFSHHPINIKTTVKNTGPLSHLAITDELPQNAELL
                     KGQNTTQHLVKHDDEVTLEYQIQFLSRGQQQFNDVSFEFSDPWGLYNSKTTQPNIFTV
                     MVHSDPKEIQKAKRVSNREHIELSTPVLGGLETLYEMDGIRPYEPGDRLKDIDWKTTS
                     RLQKHMTKIFQKQDMIETTILLDCSRSMRRTSGKSSKVEHATTLAVHLSKILQSLRHP
                     VGLVAYDEFKTLTMIEPTNNYTKFFESLSSIPEQIHTKEYSLEPLLETEENTVHVREE
                     HQQFLSTVFPFLTKGKRTIHHPAQASGIYEAVHHLLLNKTTKKHLIVITDLETDLTSL
                     Y"
BASE COUNT          912 a          750 c          493 g          822 t
ORIGIN      
        1 ggtagtatgt aatcggttac atcatcccca ctcatgctgc atatcagcaa cacaaataca
       61 ttacattaca attacaaatg taatattatt tttatttata aagatagcga ttttaacagt
      121 attttcgaac gtaaatgggg actgataaaa tagctccctc catcgtctca gattcactct
      181 gcatcatgag aaacagcgca gggggagaga tttgtattcg ttaccatatt tgatgatttt
      241 tttgtaagat accgctagac cttgtgacca aatgagaaat agaaataaat agagggacca
      301 tggaaaaaaa acactgagcg atcttgctaa tcttatcact gtacgcttgc gatgttgttg
      361 gttgtcgttg cttgaagatt gttaaatggt cgctgtcttt cgcttatgct ggtgattctt
      421 actgtgacgt ccagtccggt tcatacgcat ctattgatat gtctaccttt ttgctattac
      481 taccatcagc gtttgtgacc cagattctca tactaccggt tccactttca tagccatacg
      541 tcataatttc gcttccgcct ggcgaccatg taggtttgcc tccaagtgct cctacaaatg
      601 tcagggattt gtccacaagt ttaatacaga atacgccttg atgctcgtct ccatcaccgg
      661 taaatacaat ttttgttcca tccggagacc agtctggatt ttgattatta acggtgatgc
      721 ttctgttcgt aagttgcacc caattgctac catcagcatt catttcgtag atatgataat
      781 tgctataccc atcaccctca gctttgacat acacaaaagc aattttattc ccgtctggag
      841 accaagatgg ctggccttcg taggagggtt catttgttac gcttctcata ccagtcccgt
      901 ctgaattcac aacatagatg ttggcatttg acaccgtagt tccgctgttt tcatccccta
      961 atgattcata cacaatcgca gatccatcag gagaccacga cgggtgattg ttcgcagcaa
     1021 cattccaggt atcagtatat gcatcgtaca ctgttttata cacaattctg gttaaactag
     1081 ttccatccgc atttatcgta taaattccac catcatttgt cgctgaaaaa gcaatcattt
     1141 ttccatctgg tgaccatact ggatcttgag ccccaagaga aaaatctgta agctgtatca
     1201 aatcgctccc atcaagattt acaacgtaaa tattccctgt agaaataaag acgatttttc
     1261 ctgcaagttc ggtttcattt tgtgctccat tattctgatt cccatcctgg tttatgtcca
     1321 gctgattccc gttttgtttt gtttcacatc cactgaaccc aactgctaat aatacaacta
     1381 atattccaat aattactaat tgttttttca tttttttccc atccaccttg ttatttcgta
     1441 atcaatcatg ttctttttca aacacaatta aattacgtaa acattacaaa agaacgcttc
     1501 aatattattt aactttagtg gaaaaaaccg tactatatat cgaaaaaaaa acatgggttc
     1561 taaaaccgtg aaaactattc tctaacacat ggaactaaag tatttttttt ctgaaaaata
     1621 cggtcagggg ggcctagttt gagagcacga gaaataggta tacgccttct ttcaggttct
     1681 ttccatctgg attcaggata tactatcctc tgattacaac cgattgcatc ctcacccacc
     1741 tcgttgaaag aagcacaggt ttaaaaagca aacacgctct atcgaacagc tgacaccatg
     1801 ttacggccgc actacacaca gtatgcaaaa accctgcttt ttctttccat tctcctcttc
     1861 ataacaagct ttctcttcac cacgcttctt cccgcattca ccgcaatgct actcatcgtt
     1921 tttatggtgt acaccaaaac acacttcaaa ccaaaccctg atcaactgat catacagaga
     1981 agaataatgg aacccctcgt tttttcccac catcccatca acataaaaac cacggtaaaa
     2041 aacacaggac cactttccca tctcgccatc accgatgaac taccacagaa cgcggagcta
     2101 ctcaaaggac aaaatacgac acaacacctc gtaaaacatg atgacgaagt cacccttgag
     2161 taccagatcc aatttctgag cagaggacag cagcagttca atgatgtcag tttcgaattc
     2221 agcgacccct ggggcttgta caattcaaaa acaacacaac ccaacatctt caccgtcatg
     2281 gtccatagcg acccaaaaga aatccagaaa gcaaaacgcg tcagcaaccg agaacacatc
     2341 gaactgtcca caccagtcct aggaggactt gagacgctct acgaaatgga tggcatacgc
     2401 ccctatgaac ctggtgacag actcaaagac atagactgga agacaacctc acgactacag
     2461 aaacacatga cgaaaatctt ccaaaaacaa gacatgattg aaacaacaat ccttcttgat
     2521 tgctcacgaa gcatgagacg aaccagcggg aagagctcaa aagtggaaca tgcgactacc
     2581 ctagctgttc atctttcaaa gatcctgcaa tcactgcgcc accccgtcgg ccttgtcgcc
     2641 tacgatgagt tcaaaacact gaccatgatc gaaccgacaa ataactacac gaaatttttt
     2701 gaatcactca gcagcatccc tgaacagatt cacacaaaag agtattcatt agaaccactc
     2761 cttgaaacag aggaaaacac ggtgcacgtc agagaagaac accaacagtt cctctccacc
     2821 gtcttcccgt ttcttaccaa gggaaaacga acgatacacc atcctgcaca agccagcggc
     2881 atctacgaag ctgttcatca tctcctcctt aataagacca caaagaaaca tctcatcgtc
     2941 atcaccgatt tagaaaccga tctcacctcc ctgtacc
//
LOCUS       DATMXJ010000157         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_13644, whole genome shotgun sequence.
ACCESSION   DATMXJ010000157 DATMXJ010000000
VERSION     DATMXJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13644"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1618
                     /locus_tag="VN377_04140"
     CDS             176..1618
                     /locus_tag="VN377_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR27612.1"
                     /translation="MPKKPRTHEIVPNQNISFPIGTIYTVENLYQVLNCSKIFGKHKK
                     NGIDINKLLKALFSYKLNDNFSIKRAHEWINRAEVLETFDLEPFGERTLYRILETIGA
                     HKEEIISDLQDTLFERYDFEHTDINMDWTSIVLHGNEASLGKYGYSRDHRPDKKQITL
                     GVSELADPINIPIGLTIEPGNTNDQKHFEKTYNQIKDRLKKDTLVIFDKGANSITNTA
                     MIRADNLQYMTSKKLNTSDDRIISRFDEYNPLTIDAEKGIRGIKILKPNSVNYLFFSE
                     SLQKEQLESRARKILSQINEAKAIQESIDCGKKLPKRFRINNLLIDVEYSIQTKLIEL
                     SEDEAVKLLEDNIINGREGFFCLKSSKNLTLAEALLTYRKKDSIEKIFNSLKNEIEIK
                     PLRVWTDNSIYGALIIGFIAQLFVSLMRYEISELKHTSTKFIKKSLSNLTVTVDSWER
                     MVKTYVYSNFDAINVMVLRSNWAVSGGETS"
     gene            1892..2086
                     /locus_tag="VN377_04145"
     CDS             1892..2086
                     /locus_tag="VN377_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27613.1"
                     /translation="MSTHSKCPGLDGIWDPEMPAAGQESKSQTNVWRKTIEQQLFNEL
                     EDECGFAKATWVACTKCCKM"
BASE COUNT          708 a          426 c          439 g          531 t
ORIGIN      
        1 gtcttttcat tttttttcgt gggtaataca tgtacttaag atcatttaca agatggggct
       61 gatgccccat gactcctgat atgacaaccc tcatcgcctc cattggaacc cccccggggg
      121 gtttaaccca agtttgccac tgaaagaata agggggttct tggtcaaatt gttttatgcc
      181 caaaaaacca agaacccatg aaattgtacc gaatcaaaat atatcctttc ctatcgggac
      241 catttataca gttgaaaatc tgtatcaggt cttgaattgc agtaaaatat tcgggaaaca
      301 caagaagaat ggcattgata tcaataagtt gctcaaagca cttttcagtt acaaactgaa
      361 tgacaatttc agcataaaac gagctcatga atggataaat cgagctgaag tccttgagac
      421 atttgatctt gaaccctttg gtgaaagaac cctgtaccgt attcttgaaa cgatcggagc
      481 acacaaagaa gagatcattt cagatctcca ggacacactt ttcgaaagat atgattttga
      541 gcataccgac atcaacatgg actggaccag tatagtcctt catggaaacg aagcgagcct
      601 cggaaagtac gggtacagcc gcgatcatcg gccggataaa aaacagatca ctctgggagt
      661 cagtgaactt gcggacccga taaacatccc gattggcctc accatcgaac ccgggaatac
      721 aaacgatcag aagcacttcg aaaaaaccta taaccagatc aaggataggc tgaaaaaaga
      781 caccctggta atttttgata aaggtgcgaa cagtatcaca aatactgcaa tgatccgggc
      841 agataacctg cagtacatga catcaaagaa actcaacaca agtgacgacc gaataatttc
      901 aagatttgat gaatacaatc ctctgacgat tgacgcggaa aaagggatta gggggattaa
      961 aatcctaaaa ccaaacagtg tgaattatct tttcttttcc gaaagccttc aaaaagaaca
     1021 gcttgaatcg agagccagaa aaatattgag tcagataaac gaagcaaaag cgatacagga
     1081 aagcatagat tgcgggaaaa aacttccaaa gcgattcagg atcaataatc tgctaatcga
     1141 tgtggaatat tctatccaga ctaaacttat tgaactatcc gaagacgagg cagtcaaact
     1201 acttgaagat aatatcatca acggcagaga gggatttttc tgcctgaaat caagtaagaa
     1261 tttgacactt gccgaggctc ttctgactta ccggaaaaaa gactcaatcg agaagatttt
     1321 caattctctg aagaacgaga ttgaaatcaa accactcaga gtatggacgg ataacagcat
     1381 ttacggggcg ttaatcattg gttttattgc tcagttattc gtctcgctga tgagatacga
     1441 aatttcagaa ctcaagcata catccactaa attcatcaaa aaatctctat cgaatttgac
     1501 agtcactgtg gattcgtggg aacggatggt caaaacgtac gtctactcca acttcgatgc
     1561 cattaacgtg atggttttac ggtcaaactg ggcagtttct ggaggtgaga catcataaat
     1621 tattgaactg tcaaattggt ttgcctgaca aaaaatatgg tcgcgatagt ttaccttttc
     1681 tatagctcat gtggctaaac tggcaaactt gggtgccagg aaaaattttc aattaggcaa
     1741 cagcctctta aaaacaatct gaaacgtagc ctgttgctga aatggcacgg ctgtgatcac
     1801 ctcaatccaa ctgtttggtg gccacgatca tatttgttat tagattattt ctaattataa
     1861 accatatgat gctatattac atcaccgaca agtgtcgacc cattcaaaat gtcccggatt
     1921 agacgggata tgggacccgg agatgccagc agccggtcaa gagagcaaat cacaaacgaa
     1981 tgtctggaga aaaaccatcg agcagcaatt gttcaatgaa ttagaggatg aatgtggatt
     2041 tgcgaaggcg acttgggtag cgtgcacgaa gtgctgtaaa atgtaaagat gccctgtatc
     2101 caac
//
LOCUS       DATMXJ010000158         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_60899, whole genome shotgun sequence.
ACCESSION   DATMXJ010000158 DATMXJ010000000
VERSION     DATMXJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60899"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..302)
                     /locus_tag="VN377_04150"
     CDS             complement(117..302)
                     /locus_tag="VN377_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27614.1"
                     /translation="MVYKYEGWTLYTRSVTLKGGRKQTIYFFSKRSPKSGTPCDLPDG
                     YAVGVNKRTSLPYLKKK"
     gene            486..557
                     /locus_tag="VN377_04155"
     tRNA            486..557
                     /locus_tag="VN377_04155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:519..521,aa:Gly,seq:tcc)
     gene            581..698
                     /locus_tag="VN377_04160"
     tRNA            join(581..620,654..698)
                     /locus_tag="VN377_04160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Leu,seq:taa)
     gene            1098..>2113
                     /locus_tag="VN377_04165"
     CDS             1098..>2113
                     /locus_tag="VN377_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HWR27615.1"
                     /translation="MIGTTFTPVSISKNISSKEEQNGRTIDGQILFAPYYGTTTYLID
                     STGAINHTWSSAYRPFTEAYWLGNGTILRPIISGGGGIQKILWDGTLAWDYRYTVSGC
                     TCHHDIRYLPNGNVLMIVWVTKTRSEAIAAGRNPDTIEGTTFTPDKIIEVHPTGTTSG
                     TVVWEWHVWDHLIQDYDSSKANYGVVGNHPELIDINFGDPFVGDWLHTNSVDYNPEFD
                     QILIDIHNFNEVWVIDHSTTTEEAAGHSGGNSGKGGDLLYRWGNPEAYDAGTSNNQKL
                     FFQHDASWIKPGLPGEGHILVFNNGNNRPEGQYSSVDEFAPPVDANGEYYLESGSAYG
                     PEDYT"
BASE COUNT          618 a          437 c          450 g          608 t
ORIGIN      
        1 cttcagagtc gttactctcg tcacccatgc ttctcacgga ttattgaaca tcatttgtac
       61 ttataaagct ttctccggtc taaaaaaaaa aagagaaaaa aaggaatcaa agagttttac
      121 ttctttttaa ggtaaggaag actggttctc ttattgacac caacagcgta cccgtctggt
      181 aaatcgcagg gagtcccgct ttttggtgag cgtttgctga agaaatagat ggtttgtttc
      241 cgtccgcctt ttagggtgac gctgcgtgtg tataacgtcc atccttcgta tttgtatacc
      301 atactgcacc tcctaacgct tctgatatct cctttgttga tttaaatctt ctcctgtttg
      361 ctcctttatg ttttttcttt tttcctgtca tctcaacaga gggatgtttg ataaaaaaga
      421 ttatgaatag taagtttatg tctacctcac aacagacatt gaaaggaaga tacaggtcgt
      481 agaatgcgga tgtgatctag tggttatgat tgaggccctc caagcctctt gcccgggttc
      541 gaatcccggc gtccgcactt ctttatttca ttgatggact gcaggggtag ccaagcatgg
      601 ccaacggcgc aggacttaag atcaaaacac aagaaaggtt cgtgtgatga gacatcctgt
      661 ctcgcaggag tccgagggtt caaatccctt cccctgcact tttttttatc gttaaatcag
      721 tagtgaaatc attagaaatt cattgaatga ataatgttat tatcgaagga gtgtgttata
      781 aaattataga attgggggaa aagcatctga tacataaggt tcttgttgct ggactccatg
      841 gattctgagg tattcacatc actgcaactg tcgatgaagg aatgaaaaca acggatggca
      901 cacagttctt catcgtcaca atggtaaaaa aataacccgt ctcttttttt tcttttatcg
      961 tttgattcta acaacatccg acttattgac atctaatatt ccaatagaca tatataccat
     1021 atgaaatatt aattagactg attcctatga gacagaacca gaaatctcaa gccctcattt
     1081 ttttgttatt ctttgtcatg atagggacca cctttacccc ggtatcaata tcaaaaaaca
     1141 tttcttcgaa ggaagaacaa aacgggagga caatcgatgg acagatcctg tttgccccct
     1201 attatggcac cacaacctat ttaattgata gcactggtgc tataaatcat acgtggtcaa
     1261 gtgcgtatcg accttttact gaagcgtatt ggctagggaa tggtactatt ctgcgtccaa
     1321 taatttctgg tggtggtggt attcaaaaaa ttttatggga tggaacacta gcgtgggact
     1381 atcgatatac agtgagcgga tgtacctgcc atcatgatat taggtattta ccaaatggaa
     1441 acgtgctgat gatcgtgtgg gtaacaaaaa cacgttctga agctattgcc gcaggacgta
     1501 acccagatac catcgaagga accacattta cccctgacaa aatcattgag gtacacccga
     1561 ctggaacaac aagcgggact gtcgtctggg aatggcacgt gtgggatcat ctcattcaag
     1621 attatgattc atcaaaagca aattacggtg tcgtcgggaa tcacccagaa ttaattgaca
     1681 tcaattttgg cgatcctttt gtgggcgact ggcttcatac gaattcagtt gattacaacc
     1741 cagagtttga tcaaatactc atagacatcc ataatttcaa tgaagtctgg gtcatcgatc
     1801 atagcacaac aaccgaagag gcggcaggcc atagtggtgg aaacagcggg aaaggaggag
     1861 atttattata tcgctggggg aacccagaag catacgatgc tggaacctct aataaccaaa
     1921 aattattctt ccaacatgat gcatcatgga taaaacctgg actcccgggt gaaggacata
     1981 ttcttgtctt caacaacggc aacaaccgtc ccgagggaca atattcttca gtggatgaat
     2041 ttgccccacc ggtggatgcc aacggagagt attacctcga atcaggttca gcgtacggcc
     2101 ctgaggacta tac
//
LOCUS       DATMXJ010000159         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50083, whole genome shotgun sequence.
ACCESSION   DATMXJ010000159 DATMXJ010000000
VERSION     DATMXJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50083"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4097)
                     /locus_tag="VN377_04170"
     CDS             complement(<1..>4097)
                     /locus_tag="VN377_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27616.1"
                     /translation="VWSEPISDQEYVRVTFEKALEKTNDITIVARSQGRSTIEVYEEN
                     TTRLLATFTNITEEHRYQIFLTDLSSCQTTFDLKTTGDAVEYDYIVDPTTGWISPTGF
                     ADPSNQWSSETRVYDGNTGTYATHAGGAGWGGFLELTLSSGIYCNRVRVFSDFGYGVV
                     DRVDIDIYNTSSWIDTYNGSISDATWSELSFSAETNVTKARFRYHYLSGGWNFWFYEF
                     AFWQGQPLTLPNCTTFNATSIDETTAILKGNISNDGGEPCQYRFQYGLNSSYGNDTTW
                     GGSEINGSEFGTMIHNLTLGTTYHYRVQARNSIGTINGSNKNFTTTLPPLGWVTPTSH
                     YDSYSKWDNEKNTYDDETDTYTQSYHAVNDPNGQWSFFIYMNHSVLLCDKVRFYAKGL
                     TTDTVKVDQADLDVKRGGVWVDVFNSTFSDKQWVEKGFIQGSVSSARIKFHVNANNGG
                     LYYELYEFDFNNSRSVPMIHNESPTNRSWGVPLRPQMNITVDNPDGSSMTISWSSNSS
                     GSWQVFGTNSSIGNGTYRQRNNNFSNNNTKYWWKIFVTDGTDSNTSLFYFSTPDLVKP
                     SSNVVAITPYWKKSSITITATATDTGWSGLKNVTLYYRFSVDNSSWGGWVSAGMDSAS
                     PWSWSFTFPNTTGYYQFYSIAADNATNTELTPGSADTRCGYETTAPSSSVSAITPYWK
                     TSAPQTLTGTASDTGPSGLKNVTLRFRYRASNASSWGGWINSGLVDADPWVAVSWNFP
                     FSNGSGVYEFYSIANDNATNAEAAPGSADTSCAYDNQTPSSSLADGSISDTTRSTFEW
                     NGAEGRHPAIIRLGTSEYYLIAAEGDLGGGGATYDGWLFTIRVWSNNGTIQKNLIDSW
                     EYDISDGYYPSICLVNGSSNIYAITYEDAGSTARKIITTRAWSNNGSMQKTILDTLSL
                     YRSTTTSYSKILNAHSNIYAIAYVNNSDGDGRLATCYITNGGDIGNTVNDTLEFNSSD
                     ALNPQMCLVDENTLALVYDGGTAGGNDGYLVTYNISSTGDITNTRADQWEFDTTMGTT
                     PNILKIWDDSESSGVNWFAIGYEDTNSDLYVKTCAINDAGMITKSWVDTQAIDTTNGD
                     FCSFALAGYNSSATTKLLLVGFSGENADGYVSSVDVTNAGMINTEIDTLEFDATDCIS
                     RPWLLKTNTTFGWILMYEGTGNDGWSCTFTLTTNEVPYWKTTSPYTIIGTGSDIGPSG
                     LKNITLWYRYRVTNTSSWGGWMRWNNAENPDTDPWGTISWSFNNPNGTGRYQFYSIAR
                     DNVTNTESAPGNPDVSCGYDDEAPISSATMIADYWMSTSSMTINATANDGGSGVRNVT
                     LFSRFSPDNISWGGWVSAGVDSVLPWSWSFMFSNGTGYYQFYSIA"
BASE COUNT         1077 a          974 c          839 g         1207 t
ORIGIN      
        1 gtgcaatgct gtagaattgg taatatcctg tcccatttga aaacataaaa ctccatgacc
       61 agggcagaac agaatcgaca ccagcactta cccatccccc ccaggagata ttatcagggc
      121 tgaaacgaga gaacaacgtg acatttctta ccccgcttcc gccatcattt gctgttgcat
      181 tgatggtcat tgaagaggtt gacatccaat aatcagcgat catggtggca gaagaaatgg
      241 gtgcttcgtc atcatacccg catgagacat ccgggtttcc aggggcagat tcggtatttg
      301 ttacattatc ccttgctatg ctatagaatt ggtatcgacc ggttccattc gggttgttga
      361 agctccatga gatagtgccc caggggtcag tatcaggatt ttcagcattg ttccatctca
      421 tccatcctcc ccagcttgag gtattggtga cccggtagcg ataccataac gtgatgtttt
      481 tcagtccact aggtccaata tctgatcctg tgccaatgat agtataagga gatgttgtct
      541 tccaataggg aacctcattt gtcgttaggg tgaatgtgca actccacccg tcattgccag
      601 taccttcgta catgagtatc caaccaaacg ttgtgttggt tttcaacaac caaggacgac
      661 tgatgcagtc agtagcatca aattccaagg tatctatttc agtgttgatc atccctgcat
      721 tggtgacatc aacactgctc acataaccgt ccgcgttctc accactgaaa cctacgagca
      781 atagtttagt ggttgcagaa ctgttataac ctgccaatgc aaaactacaa aaatcaccat
      841 tggtggtatc gatcgcttgg gtatcaaccc aactctttgt aatcatccca gcatcattga
      901 ttgcacatgt ctttacgtac aaatcactat tcgtatcctc atacccaatc gcaaaccaat
      961 tgactccgct gctctcggaa tcgtcccata ttttaaggat attaggggtt gtacccattg
     1021 ttgtatcaaa ttcccattgg tccgctcgtg tattcgtgat atcccctgtg gatgatatat
     1081 tgtaggtgac aagatacccg tcattgccac cggcagttcc gccatcatat accagagcaa
     1141 gcgtattttc atcaacaagg cacatctgtg ggtttaacgc atcactgctg ttaaattcaa
     1201 gggtgtcatt tactgtatta ccaatatccc ccccattggt gatataacat gttgcaagac
     1261 gaccatcacc atcgctgtta ttcacgtagg cgatcgcata gatgttgcta tgtgcattga
     1321 gtatttttga gtaacttgtt gtcgtgctcc tatacagtga aagggtatca agaatggttt
     1381 tctgcatact tccattgtta ctccaggcac gggtcgtgat gatttttcgt gcggtactcc
     1441 cggcatcctc gtaggtgatt gcgtagatat tcgaactacc attcaccagg cagatactcg
     1501 gataatatcc atcactgata tcgtactccc aactatcgat aagatttttt tgaatcgtac
     1561 cattattgct ccagactctg atggtaaaaa gccaaccatc ataggtcgcg ccaccgccac
     1621 ccaagtcccc ttcagcagcg ataagataat actccgaggt ccctaaacga atgatagctg
     1681 gatgtcttcc ctccgcacca ttccattcga aggttgatct cgttgtgtca ctgatacttc
     1741 catcagctaa ggatgatgac ggggtttgat tatcgtatgc acatgatgta tctgcagatc
     1801 ctggggcggc ctcagcgttt gtcgcgttat cgttcgcgat gctataaaac tcatacactc
     1861 cgcttccatt cgaaaatgga aagttccacg acacagcaac ccaggggtca gcatcgacta
     1921 atcctgagtt tatccatcca ccccaacttg aagcatttga cgctctatat ctgaatcgga
     1981 gcgttacgtt cttcaatcca ctcggccccg tatctgatgc tgtcccggtc agggtttgtg
     2041 gtgctgaagt cttccaatag ggggtaatag cagacactga agaactcgga gccgtagttt
     2101 catacccaca cctggtatca gcactacctg gtgttaattc ggtgttcgtc gcattatcag
     2161 cagcgatact gtagaactga taatatcctg ttgtgttagg gaatgtaaag ctccaagacc
     2221 agggtgaagc agaatccata ccagcactga cccacccacc ccaactggaa ttatcaacac
     2281 tgaagcgata gtacaacgtg acattcttca acccactcca acccgtatct gttgcagtcg
     2341 cagtaattgt tatcgagctt ttcttccagt acggtgtgat agcgacaaca tttgaactag
     2401 gttttacgag atccggcgtc gaaaaataaa acaaagatgt attcgaatca gtcccgtcag
     2461 taacaaagat tttccaccaa tatttggtat tgttgttgct gaaattatta ttcctctggc
     2521 gatatgttcc gtttccgatg gagctgttgg tgccaaagac ttgccaggag ccgctactat
     2581 tgctcgacca gctgatcgtc atgcttgacc catctggatt gtctacagta atgttcattt
     2641 gtggtcgaag gggaaccccc cagcttctat tagttggact ctcattgtgt atcattggaa
     2701 ccgatcttga attattgaaa tcaaactcat agagctcata atacagacca ccattgtttg
     2761 cgttcacatg aaatttaatc cgtgcggagc tcacacttcc ctggataaat cccttttcca
     2821 cccattgttt atcagaaaat gtactattga aaacatcaac ccacaccccg cctcgtttaa
     2881 catctaggtc tgcttgatca acttttacgg tatctgtcgt aagccccttg gcataaaaac
     2941 gaaccttatc acatagtaaa acactatgat tcatatagat gaaaaaactc cattgtccat
     3001 ttggatcatt gacagcatga tatgattgag tataggtatc tgtctcatca tcatacgtat
     3061 ttttctcgtt atcccatttg gaataagaat cgtaatgact ggttggcgtg acccacccta
     3121 gtggtggtag cgttgttgtg aaatttttat ttgatccatt tatcgtacca atgctattac
     3181 gagcctgtac cctatagtga taggtagtcc ctaatgttag attatggatc attgtaccaa
     3241 attctgagcc gtttatttcg cttccccccc aggtggtatc gtttccgtag gaagagttta
     3301 aaccatattg aaagcgatat tggcaaggtt ccccgccatc attggagata tttcctttta
     3361 atatcgcagt tgtttcatca attgatgtcg cattaaaagt tgtacagttc ggtaatgtca
     3421 gtggttggcc ttgccaaaaa gcgaattcat aaaaccagaa attccagcca ccagataaat
     3481 aatgatatcg aaatcgagct ttggtgacgt ttgtttccgc gctgaaactt aattcactcc
     3541 aagtcgcatc tgaaattgaa ccattatatg tatcaatcca gcttgaggta ttgtagatat
     3601 ctatatcaac tcggtcgaca actccatacc caaagtcaga gaagacgcga acgcgattac
     3661 aatagattcc actgctaagg gttaattcta aaaatcctcc ccatccggct ccgcctgcgt
     3721 gggtagcata tgttccagta ttcccatcat acactcttgt ttcgcttgac cattgattac
     3781 tggggtcagc aaaaccagtt ggggagatcc atccggtcgt tggatcgacg atatagtcgt
     3841 actcgacagc atcacccgta gttttgagat cgaaggtggt ttggcagctg ctgaggtctg
     3901 tgagaaaaat ctgatatcgg tgttcttcgg tgatgttggt aaatgttgca agaagcctcg
     3961 tcgtattctc ttcataaacc tcgatggttg atctaccctg gcttcgtgca acaatggtaa
     4021 tatcgtttgt tttttctaat gctttctcaa acgttacccg cacatattcc tgatcggaga
     4081 ttggttctga ccatacc
//
LOCUS       DATMXJ010000160         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_34485, whole genome shotgun sequence.
ACCESSION   DATMXJ010000160 DATMXJ010000000
VERSION     DATMXJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34485"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VN377_04175"
     CDS             <1..830
                     /locus_tag="VN377_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWR27617.1"
                     /translation="SVGKKFNQKYIEGGFHFGYYEKGVVNYRQAIQNMNDYIGRLLDI
                     GTGPAFEVLEAGCGIGATSRYLAKKYPGCHFTGISLGIEEIELAKKIQKEQNLTNITF
                     LSGNYKNTGFQDTSFDRIFALESIVYAVQKKDVINEISRILKPHGKCVILDGFFPKNQ
                     PSSSFLKKAYRLVLSKRSIPGIVSLLEMQSYLESAGFTDIAIHNLSKNISKHYLFGGF
                     FVSLRGLMSAEVKRLRNKVKGKTIEDTDKIIGGAGFLEMLLGITKNLGYYAIVATKK"
     gene            919..2268
                     /gene="thiD"
                     /locus_tag="VN377_04180"
     CDS             919..2268
                     /gene="thiD"
                     /locus_tag="VN377_04180"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWR27618.1"
                     /translation="MVKKVVLSIAGSDSSAGAGIQADLKAFSYLNLHGVTAITCVTAQ
                     NTQQVRTIYKLPVDIIEEQIESLFDDFTIAAVKTGMLYDEEIVHVVTQQLKKHHLKPV
                     VDPVMVATSGDALSNHTFLASLKKELLPHAYMVTANIPEACALADMKHLRQKDLENAA
                     LKIFELGPEYVLLKGGHLQGEMVTDLLYDGKQFYPYTLPRISRRKAHGSGCTLSALIT
                     GLLASGESPVTAVNKAKSIVWSMIQEGYAPGKGADMLNHSSTLQIPPAINNEEQFDVW
                     LELKNVVQTLISYIPPQFIPEVGMNFVYAVPNAKTRNDICGIEGRITRYKDRALLCGT
                     LDFGASKHVASIVLAAMSFDQNQRSALNIRFTQNTLQLCTRLGFTLGSFDRKNEPENA
                     SSTMEWGTKQAIMTCGCVPDIISDTGAVGKEPMIRVLGKNPQDVLAKIKKIIDASYS"
     gene            2352..2639
                     /gene="albA"
                     /locus_tag="VN377_04185"
     CDS             2352..2639
                     /gene="albA"
                     /locus_tag="VN377_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HWR27619.1"
                     /translation="MTAKEESEENVIYVGNKPPMSYVLAVVTQFNSGSTEVVIKARGR
                     AISRAVDAAEITRNRFVQDAKIKEIRIGTEAITNEEGRTSNVSSIEISLSK"
BASE COUNT          900 a          625 c          573 g          637 t
ORIGIN      
        1 ggtcggtggg gaaaaaattc aatcaaaagt atatcgaagg tggttttcat ttcggatact
       61 atgaaaaagg tgtcgtaaac tatcgacaag caattcaaaa tatgaacgat tacatcggca
      121 gactccttga tataggaacc ggacctgcat tcgaggtgtt agaggcgggc tgcggaatag
      181 gggcgacatc tcgatactta gcaaaaaaat atcctggatg tcattttacc gggatatccc
      241 tcgggataga ggaaattgag ctagcaaaaa aaatacaaaa agaacagaat ctaacgaata
      301 taacattcct ttcagggaat tacaagaaca caggcttcca ggatacctcg ttcgatcgaa
      361 tctttgccct cgaatcaatc gtctatgcag tacagaaaaa agatgtgatc aatgaaatct
      421 cacgaatcct caaacctcat ggcaagtgtg tgatcctcga tggtttcttc ccgaaaaacc
      481 aaccttcgag ttcatttcta aaaaaagcct ataggttagt gctatcgaaa cgttccatcc
      541 caggcatcgt ttcattacta gaaatgcaat catacctgga atcagcgggg ttcacagaca
      601 tcgctatcca taatctttca aaaaacatca gcaagcatta tttatttggt ggtttctttg
      661 tgagtctcag aggtttaatg tccgctgagg tcaaacggct cagaaataaa gtaaaaggaa
      721 aaactattga agacactgat aagataatcg ggggagctgg ttttcttgag atgcttctgg
      781 gtataaccaa gaatctcggg tattacgcga tcgttgcgac caaaaaataa acgatataac
      841 gggcagaaaa atatcataga aaaaccatct tcttcctaga aaaatccata taaatctcca
      901 cggtattgct catcaggtat ggtcaagaag gttgtgctca gcatcgcagg ctcggattcc
      961 tcagcgggag caggtatcca agcagatctg aaagcattct catacctgaa cctgcatggt
     1021 gtcaccgcca tcacctgtgt cactgcacag aacacgcaac aggtcagaac gatctacaaa
     1081 ctccctgttg atatcattga agagcaaatc gaaagcctgt ttgatgattt cacgattgct
     1141 gctgtgaaga ctgggatgct gtacgacgaa gaaatcgtcc atgttgtgac acaacaatta
     1201 aaaaaacatc atctcaaacc agtagtcgac ccggtgatgg tcgcgacgag cggtgatgct
     1261 ctttcgaacc acacatttct tgcatcatta aaaaaagaac tcttacctca tgcatacatg
     1321 gtaacggcaa acatccctga ggcatgcgcc ctagcagaca tgaaacatct cagacaaaaa
     1381 gacctcgaga acgcggctct gaaaatcttt gaactcggtc cagaatacgt cttgctgaaa
     1441 ggcggccatc tccaaggaga gatggtgaca gacctcctct acgatgggaa acagttctat
     1501 ccctatacac tccccagaat atctcgtaga aaagcacacg gttcaggctg cacgctgtcc
     1561 gctctgatca ccggattgct agcatctgga gaatcgccag tcaccgcggt aaacaaagca
     1621 aaatccatcg tctggagcat gatacaagaa ggatatgctc ctgggaaggg cgctgatatg
     1681 ctgaaccatt cctccaccct tcaaatccca ccagccatta ataacgagga acaattcgac
     1741 gtctggttgg aactaaaaaa cgtggttcaa acactgattt cttacatccc accacagttc
     1801 atccctgagg tgggcatgaa cttcgtctac gctgtcccca acgcaaaaac acgaaacgat
     1861 atctgcggaa tcgagggaag aatcaccaga tacaaagaca gagccttgct atgcggcact
     1921 cttgatttcg gtgcctccaa acatgtggca tcgattgttc ttgcagccat gtcgtttgac
     1981 cagaaccaac gatccgcact gaacattcgg ttcacacaaa atactcttca gctctgcaca
     2041 cgccttggtt ttacgctcgg ctcatttgac aggaagaacg aaccagagaa tgcttcctcg
     2101 accatggaat ggggaacaaa acaagcaatc atgacatgcg gatgtgtccc agatatcatc
     2161 tcagatactg gtgctgtcgg aaaagaaccg atgatccgcg tacttggtaa aaatccgcaa
     2221 gacgttcttg ccaaaatcaa gaagataata gacgcttcat actcataaga tacaaaatta
     2281 cgaaacatat ataaatgatg acgcccatag gaccgcccac taacctagaa acttacggag
     2341 aggtatagtt tatgacagca aaagaagaaa gcgaagaaaa cgtgatctat gtaggaaaca
     2401 aaccaccaat gagctacgtt ctggcagtcg taacgcaatt caacagcggt tccaccgaag
     2461 tcgtcatcaa ggcacggggc cgagcgatta gcagagcggt cgatgcagca gaaataacca
     2521 gaaaccggtt tgtccaagat gcaaagatca aagaaatacg catcggaaca gaagcaatca
     2581 ccaacgaaga aggacgaacc tcaaacgtct catcaatcga gatatctcta tcgaagtaaa
     2641 caggagtatt atccaagaat aatacttctt tccttttatt tttctattga ggtgtacact
     2701 atatcattat ttttttttaa aaaaaaaaaa agaaa
//
LOCUS       DATMXJ010000161         6619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_60943, whole genome shotgun sequence.
ACCESSION   DATMXJ010000161 DATMXJ010000000
VERSION     DATMXJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60943"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /locus_tag="VN377_04190"
     CDS             <1..1762
                     /locus_tag="VN377_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HWR27620.1"
                     /translation="HTIVIPKELEEEPVDSIFTILRHRKDEDIKTIDLGDPIQKYRII
                     FDNSAVAIMLTDENERIVHWNKYTEKLLGMSGEELIMRPVKYLYPPEEWKKIRSENIR
                     QKGIQYHMETKMIRNDNELINVDISLSVLKDRIGNVIGSIGIVKDNSQNKQMEKALRT
                     SEEKFKQLYEKAPIPYHTLSPDGAITDVNEKWCKLFGYTKEEVIGNSIFDYIHRDEQE
                     IAKSSFKEKINSKKPYTGGHERTYITKNGEPRVFVIHDFLSYNETGGMLSVYSTMEDV
                     TGRKKIEEELQKAHYWLEKKVQERTVELSKENLLLKKRNNEYKRTIGELHVQLEKLQK
                     SQKKIEQMNTRLKKLDHIKSNFLNVTSHELRMSMSTIKGYVEILLMKSLGPITEEQRK
                     GLDVILTNTDRLDQLIQDTLDVSRLESGTMKFIPEKTDIGRLIQNATGTIQSAADVKQ
                     IKIIKEIDEEIPEMNIDQERIKQVLVNLLQNAISVSSEGSKISIRVNKEPEEILFEIQ
                     DFGQGIPKNKQKKIFDIVYQIDSGIDDLGFTIARGIVLSHGGKIWVDSEQGQGSIFRF
                     TLPITPIQDQEGKFKKVDIF"
     gene            1797..2726
                     /locus_tag="VN377_04195"
     CDS             1797..2726
                     /locus_tag="VN377_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWR27621.1"
                     /translation="MKKDTTNSSGAGHLFEYRHVEQKKLMVSLSITLAAMVVELVGGY
                     LTNSMALLSDAGHMFTHAFALIIGLTAILIARKPPCHHQTFGLYRAEVLAAFVNGLFL
                     FAVAGIIIYEAILRLLHPIEILGLEMLAIAFIGLVVNILSILILRGAHQKDLNIRSVF
                     YHMFADAISSVGIVIAALVIMYTNWIFIDPIVSIGISIIIVFWAWGVLRDSTRILLET
                     APKGLNIDMISEDLKRNFLEIRELHNIHLWSITPDMLVFSAHMQFDPSKVQGIQEESI
                     SKINDYLLKKYNIIECTLQICTDQGSEVCNISK"
     gene            complement(2744..3223)
                     /locus_tag="VN377_04200"
     CDS             complement(2744..3223)
                     /locus_tag="VN377_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HWR27622.1"
                     /translation="MKKKIVVMSIITIGAIVFLAGCTGTTQNKNNSNPNTNSNSNLQQ
                     NETEVRIPISDISTTATFYPYDSNGVTVRYFSVKDKQGSVHIAFDACDVCYEAKKGYK
                     QNGDLMQCLNCGKTFSITSIGTENTAGGCWPSFLPMNLDGNDVVIKIADLEAKSFMF"
     gene            complement(3240..3929)
                     /locus_tag="VN377_04205"
     CDS             complement(3240..3929)
                     /locus_tag="VN377_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27623.1"
                     /translation="MSEEIIRLNNIKKYYDENTHVLENLNLIINKGAWTSIMGPSGSG
                     KTTLLNIIGCLDSATAGSVSINGTEITGLSQTQLTRFRRENIGLVFQQYHLVPYLTAL
                     ENVMLAQYYHGAIDEAKATEVLKRVGLEHRLDHVPSHLSGGEQQRVCIARALINEPKI
                     LLADEPTGNLDQKNGEIVLDLIRQLHAEGHTIILVTHNPEIAKLGQRIITVIDGKICS
                     DSACTNEINEK"
     gene            complement(3934..5184)
                     /locus_tag="VN377_04210"
     CDS             complement(3934..5184)
                     /locus_tag="VN377_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWR27624.1"
                     /translation="MRILTKSIVKRKSRIAIAVISVIIGASIATALLSVSLDVSEKVG
                     IEFRKYGANLMVVPKSDTIEVGFPGVEFGSVTEQRYINESDLWKIKTIYWRNNVLGFA
                     PFLYQVVSVGDGVNRHNVVLAGTYFDKNVVILQPYSPNDNPMFITGIQKISPWWTVNG
                     SWISDQNDTTSAMIGIKVAEALHLHISDSFTIHYDEYVGDAINETSHNLTVVGIIETE
                     GYEDNQIFVNLQVAQELTHRPGKLHTIQVSALCNACPVEAFVEEIQANIPYVEARTVK
                     QLASAEMNILGKIENMMFLITIVGLIASALGVMTTMITSVIERQKEIGLMKSVGAENK
                     KIISLFLSEATIIGVIGGILGYIIGIILAQFIGYSVFSMSITPRLEVIPISVGISIGV
                     ALLASILPVRRAVRVEPAIVLRGE"
     gene            complement(5273..5746)
                     /locus_tag="VN377_04215"
     CDS             complement(5273..5746)
                     /locus_tag="VN377_04215"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27625.1"
                     /translation="MISERKENSRILLISIVLLFIGTSILPMTLSIGSAQLTRNEPPT
                     VSIVKPVEKSFYIRDFRLLPSLRTHILGFITVKVNAADDVEVKQVEIYIDGELKNIST
                     TVHSCGSYMWIWNERSWLQSRHTLKVIAVDTDGLTAEDTREVVLHNFPILHPLYP"
     gene            5821..6138
                     /locus_tag="VN377_04220"
     CDS             5821..6138
                     /locus_tag="VN377_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWR27626.1"
                     /translation="MTIKIDCEENVWYLLPLIRKEFAKSLIKDHGLTQRKAAERLGIT
                     EAAVSQYVSKKRGDLKVTNAEIKREIKTSTKRIMNGDLQVMKTETCRICHLLRVRGVG
                     KER"
     gene            complement(6140..>6619)
                     /locus_tag="VN377_04225"
     CDS             complement(6140..>6619)
                     /locus_tag="VN377_04225"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27627.1"
                     /translation="TLSAIDLDPENLMIFSVLFNAEKHTGYSQAPDKNPFEAHYVDAV
                     NATRVIQGGNLPPEIGIQNPAVKYFHRFGNPIRKTLTGKTVLVGKTIIVASAHDDSQI
                     EKVEFYINNKLMATVTQDPFQWTWHKFSFGKKTITIKAYDDSGKQSTASMDVVAFML"
BASE COUNT         1974 a         1351 c         1380 g         1914 t
ORIGIN      
        1 acatacaatc gtcatcccaa aagaattaga agaagaaccc gttgattcga ttttcactat
       61 tttacgacat cggaaggatg aggacataaa aaccattgat cttggtgacc ctatccaaaa
      121 atatcggatc atttttgata attcagcggt cgccatcatg ctgaccgatg agaatgaacg
      181 gatagtccat tggaataaat acacagaaaa actcctcggg atgagcggag aagaactcat
      241 catgagacca gtcaaatatc tctaccctcc ggaggaatgg aagaaaatac gatcagaaaa
      301 catcagacag aaaggcatcc agtaccacat ggaaacaaaa atgatacgga acgacaacga
      361 actcatcaat gtagatatct cccttagtgt actaaaagac cgcatcggga acgtcattgg
      421 ctcgattgga attgtgaaag acaactcaca aaataaacag atggaaaaag cgttacgaac
      481 atccgaagag aaattcaaac aactctacga aaaagcaccg attccatacc atacgttatc
      541 accagatgga gcgattaccg atgtgaacga aaaatggtgc aaattgttcg gatatacaaa
      601 agaagaagtc attggaaatt ctatttttga ttacattcat agggatgaac aagagatagc
      661 taaatcctct tttaaggaga aaatcaacag taagaaaccc tacaccggtg gtcatgaacg
      721 aacctatatc acaaagaacg gtgaaccacg ggtgtttgtc atccatgatt tccttagtta
      781 taatgagacc gggggcatgc tctcagtcta ttcaaccatg gaggatgtca cagggaggaa
      841 aaaaattgaa gaagaactcc aaaaagcgca ctattggtta gaaaaaaaag tccaagaacg
      901 cactgttgag ttatcaaaag aaaacttgtt gttaaaaaag agaaacaatg aatataaacg
      961 caccatcgga gagcttcatg tccaacttga gaaacttcag aaatcacaga aaaagataga
     1021 acagatgaac actcggctga aaaaacttga tcatatcaaa tcgaatttcc tgaatgttac
     1081 ctcacatgaa cttcgaatgt caatgtcgac cattaaagga tatgtcgaga tccttcttat
     1141 gaaaagtctt ggccccataa cagaagaaca aagaaagggt ctcgatgtga ttttaacaaa
     1201 cacagataga ttggatcaac ttatccagga tactcttgat gtctcccgac ttgagtcagg
     1261 taccatgaaa ttcatccctg agaaaacaga tatagggaga ttaatccaaa acgccacagg
     1321 tacgattcaa tccgctgcag atgtcaaaca aataaaaatc atcaaagaaa ttgatgaaga
     1381 aatcccagaa atgaatatcg accaagaacg tatcaaacaa gtacttgtca acctcctcca
     1441 aaatgccatt agtgtttcat ccgagggatc gaaaatctcc atcagagtga ataaagaacc
     1501 tgaggagatc ctatttgaga tacaagattt cggtcaagga ataccaaaga acaaacagaa
     1561 gaagattttt gatatcgtgt atcaaataga ttctggaatc gatgatctag gatttaccat
     1621 tgctcgtggt attgtgctct cccatggtgg aaaaatatgg gttgatagcg aacagggtca
     1681 aggaagtatt ttccggttca ccttaccaat cacacctata caggatcaag aaggaaaatt
     1741 caaaaaagtt gatatctttt gatccgagta tcaacaagta agagaagacc aaggctatga
     1801 agaaagatac gacgaacagt tcaggtgcgg ggcacctctt tgaatatcga catgttgagc
     1861 aaaaaaaact catggtgtcg ctttctatca cccttgccgc aatggttgtt gaactcgtgg
     1921 gagggtatct gaccaatagc atggcattgc tcagtgatgc gggtcacatg ttcacccacg
     1981 cgtttgcact gattattggt cttaccgcga tacttatcgc ccgcaaacca ccgtgtcatc
     2041 atcaaacgtt cgggttatac cgcgctgagg tgctcgctgc atttgtcaac ggattattcc
     2101 tctttgctgt cgccggaatc atcatctacg aagcaatcct aagactgctg catcctattg
     2161 aaatccttgg tctagaaatg ctcgctattg cattcatcgg cttagtcgtc aacatcttaa
     2221 gtatccttat cctcagggga gcccatcaga aagatctgaa tatacgaagt gtgttttatc
     2281 atatgtttgc tgatgcgata tcctccgtcg gaattgtcat cgcagccctt gtcattatgt
     2341 atacgaactg gatcttcatc gatccgatcg tcagcatcgg tatttccata attatcgtgt
     2401 tctgggcgtg gggtgtgtta agagattcga caaggattct cctagaaaca gcgccaaaag
     2461 gcctgaacat cgacatgatt tctgaggatc tgaaaaggaa ttttctagaa attcgagaac
     2521 tccacaacat ccatctttgg tcgatcaccc cagatatgct tgtgttttcc gctcacatgc
     2581 agttcgatcc aagcaaagtc caggggatac aagaggagag catctcaaaa ataaatgact
     2641 atcttttgaa gaaatacaac atcatcgaat gtacacttca aatctgcacg gatcagggct
     2701 ctgaggtctg taatatctcg aagtaatatc actgattttt cggctagaac ataaagcttt
     2761 ttgcttctaa atcagctatt tttatgacca catcgtttcc atcgagattc atcggtaaaa
     2821 aactcggcca gcagccacct gctgtatttt ctgttccaat acttgttatg gaaaatgttt
     2881 taccacagtt gagacattgc ataagatccc cgttttgttt gtatcctttt tttgcttcgt
     2941 aacacacgtc acaggcatca aatgcgatat gtacagatcc ctgtttatct ttcactgaaa
     3001 aataccggac agtgacgcca ttagaatcat aaggatagaa tgttgctgtc gtactgatat
     3061 cagagattgg gattcggacc tcggtttcgt tttgctgaag atttgaattg gaattcgtat
     3121 tcgggtttga attattttta ttttgcgtcg ttccagtaca tccagcgaga aatacaatag
     3181 cgcctattgt tatgatgctc ataaccacaa tttttttctt catttttttt ccaccttttt
     3241 tatttttcat ttatttcatt tgtacatgca gaatccgaac aaattttacc atcgataacc
     3301 gtgattattc gttgtcctag ttttgctatc tccgggttgt gtgtcacaag aataatcgta
     3361 tgtccctctg cgtggagttg tctgataagg tccagcacaa tctccccgtt tttctgatca
     3421 agattcccag tcggttcatc agccaacaat atttttggtt cattgataag tgcgcgtgcg
     3481 atacaaaccc gttgttgttc acctccggag aggtgagaag gaacgtgatc caatcgatgt
     3541 tcgagtccca ccctttttaa cacttcagtt gcctttgctt catcaatggc cccatggtag
     3601 tattgtgcca acatcacatt ctctagtgcc gtgagatagg gaacgaggtg gtattgttgg
     3661 aaaaccaatc cgatgttttc tcgtcgaaat cgtgtcagct gtgtttgact aagtccggtg
     3721 atttccgttc catttataga cacggaacct gcggttgctg aatccaaaca tccgatgata
     3781 ttcaacagcg ttgtttttcc cgaaccagat ggtcccatga ttgacgtcca agctcctttg
     3841 ttgatgatga gatttaggtt ttcaagaaca tgggtgtttt cgtcgtagta tttttttata
     3901 ttatttagtc gtatgatttc ctcgctcatg atcttattca cctcgtagga cgattgcagg
     3961 ttcaacacgg acggctctcc gaacggggag tatacttgca agcagtgcta ctccaatgga
     4021 gattccaacg gagatcggaa tgacttctaa tcggggggta atcgacatgg aaaaaacact
     4081 atagccgata aattgcgcta ggataattcc gatgatatat ccaagaatcc caccaatcac
     4141 cccaataatg gttgcttcag agaggaagag ggaaatgatt tttttgttct ctgctcctac
     4201 gcttttcata agtcctattt ctttctgtcg ttcaataacg gaggtaatca tcgtggtcat
     4261 gacgcctagt gcagatgcaa ttaatccaac aatggtgatg agaaacatca tattctcgat
     4321 tttaccaagg atgttcatct cagcacttgc gagttgtttt actgttcttg cttcgacata
     4381 gggaatattt gcttgtattt cttctacgaa cgcttcgaca ggacaggcgt tgcataacgc
     4441 ggagacttgg attgtgtgca gttttcctgg tcgatgggtt aattcctggg cgacctgtaa
     4501 attgacaaag atttgattgt cctcgtatcc ttcggtttcg atgataccga cgacagtgag
     4561 gttatggctg gtttcattaa tagcgtcacc aacatattca tcgtaatgta tcgtaaagga
     4621 atcactgatg tgaagatgga gtgcttctgc aacctttata ccgatcatgg cgctggttgt
     4681 atcattttgg tctgagatcc atgagccatt gaccgtccac caggggctga ttttttgtat
     4741 cccggtgatg aacataggat tgtcgttggg ggagtatggt tgcagaatca cgacgttttt
     4801 gtcaaaatag gttcctgcaa gcaccacgtt gtgtcgattc actccgtctc caacactcac
     4861 gacttggtat aagaacggtg cgaatcctag cacattattt cgccaataga ttgtttttat
     4921 tttccatagg tcgctttcgt tgatgtagcg ttgttcggtg actgagccaa attctactcc
     4981 gggaaatccg acttcgatgg tatctgattt gggaaccacc ataaggttgg caccgtattt
     5041 ccgaaattca ataccgactt tttcactgac atcaagggat acgctgagta atgcggttgc
     5101 gatggatgca ccgatgataa cggagattac tgcgatagct attcttgatt tccgtttgac
     5161 gattgatttt gtaagtatac gcatgaacat tttgtcacca actacagctt aacaattgtt
     5221 aagtatttat attgtcttta gtcatatata taatttttgg tatcgagaag ttctagggat
     5281 acaggggatg taaaatcggg aaattatgga ggacaacttc acgggtatct tctgctgtta
     5341 aaccatcggt atccactgcg attactttta aggtgtgtct tgattgtagc caagaacgct
     5401 cgttccaaat ccacatgtac gatccacaac tatgaaccgt tgtactaata ttttttaatt
     5461 ctccatcgat atagatttct acctgtttta cttcaacatc atctgccgca ttgactttga
     5521 cggtgataaa tcccaagata tgggtccgca gcgatggcaa aagacgaaag tctcggatat
     5581 agaagctttt ttctacaggt tttactattg aaacggttgg gggttcattt cgggtgagtt
     5641 gtgcgctacc tatacttagg gtcattggga gaatgcttgt tcctatgaac aataatacga
     5701 tagatattag tagtatcctt gagttttctt ttcgttctga tatcatttgt tcacccactt
     5761 agcttaacaa tcgttaagta cttatatgga aagatgatat atataataat tgtggtaagt
     5821 atgaccataa aaattgattg cgaggaaaac gtctggtacc tcctcccgct catcaggaag
     5881 gaattcgcaa aaagtctaat taaagatcat ggccttactc aaagaaaagc cgccgaaaga
     5941 ctagggataa ccgaagcagc agtctcacaa tatgtctcaa aaaaacgagg ggatttgaaa
     6001 gtaacaaacg cagaaataaa aagagaaatc aaaacatcaa caaaacgaat catgaacggt
     6061 gacctacaag tcatgaaaac agaaacctgc agaatctgcc atctgctacg cgtccgaggc
     6121 gttgggaaag aacgatgaac taaagcataa atgcaacaac atccatactt gctgtcgatt
     6181 gttttcctga atcatcatat gctttaatcg taatggtttt ttttccaaaa ctaaacttgt
     6241 gccaggtcca ttggaacgga tcctgtgtca cggtcgccat gagcttattg ttgatataaa
     6301 actccacctt ttcgatctga gaatcatcat gggccgaagc aacaatgatc gtcttaccta
     6361 caagaactgt ctttcctgtc agcgtctttc gaatgggatt gccaaaccga tggaagtatt
     6421 tcaccgcagg gttttgtatg ccgatttctg gtgggaggtt acctccttgt atcactcgag
     6481 ttgcattcac ggcgtcgaca taatgggctt caaacggatt tttatcaggc gcttgggaat
     6541 atcctgtgtg tttttctgca ttaaacagca ctgaaaagat cataaggttc tcggggtcaa
     6601 ggtctatagc actcagggt
//
LOCUS       DATMXJ010000162         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28661, whole genome shotgun sequence.
ACCESSION   DATMXJ010000162 DATMXJ010000000
VERSION     DATMXJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28661"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..242
                     /locus_tag="VN377_04230"
     tRNA            168..242
                     /locus_tag="VN377_04230"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:203..205,aa:Lys,seq:ctt)
     gene            418..705
                     /locus_tag="VN377_04235"
     CDS             418..705
                     /locus_tag="VN377_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27628.1"
                     /translation="MYQNRTIDGKRKWVPTAWCCTECHFLYTVASDTLMYPIGGKEYN
                     VSYSGKCPNCDLKLTRLFRHKNPSQGKQEWISSAWYCNRCKYVWLDKEEKS"
     gene            896..1034
                     /locus_tag="VN377_04240"
     tRNA            join(896..933,1000..1034)
                     /locus_tag="VN377_04240"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:930..932,aa:Trp,seq:cca)
     gene            1072..1284
                     /locus_tag="VN377_04245"
     CDS             1072..1284
                     /locus_tag="VN377_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27629.1"
                     /translation="MKPSLKNINAYTIVALIVLIAGLLLYITWGLRYNVWADIGIYSI
                     TIVLVLGGLLGAILSLTYERIEDGQE"
     gene            1343..1534
                     /locus_tag="VN377_04250"
     CDS             1343..1534
                     /locus_tag="VN377_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HWR27630.1"
                     /translation="MEQEEMLRKYSGEWILLFNDEVVDHSVNLEDVLRAADEKFPTKK
                     FPADNVKISKVLSGSIHLH"
     gene            1542..1943
                     /locus_tag="VN377_04255"
     CDS             1542..1943
                     /locus_tag="VN377_04255"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWR27631.1"
                     /translation="MPETPFVFEYKNYPLIPIKFSIRDRETPMIDALLDSGGDFIVIP
                     YAIAKYLGLKLVKAGCVDTAAGETTLYKTVVNMVIFDEDQKYTYENLEVHVSDRNDLP
                     VLLGRSPIFEDHEIIFQKHQNKLILQPIQHQ"
     gene            2025..2210
                     /locus_tag="VN377_04260"
     CDS             2025..2210
                     /locus_tag="VN377_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27632.1"
                     /translation="MPYIYEGWTLYKRDVTLKGGRKQTIYFFSKRSPKSGTPCELPPG
                     YGVGVNKRTGLPYLKKK"
     gene            complement(2566..3741)
                     /locus_tag="VN377_04265"
     CDS             complement(2566..3741)
                     /locus_tag="VN377_04265"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27633.1"
                     /translation="MKTKRKILPLMTAAIIMIILQTSTLACTIFSAQDEIMTLAGNNG
                     DYSDDDTYIVFYPAENGKHGRMYAGWQQFWWQTGINDQGLFFASASTSYLEVQNSTQN
                     PHPPRYLMYICMEQCSTVEEVLEVFDQYNLDFLETMQLMVSDATGASVIIEGDPMHIK
                     QDYYQVVTNFRLSQTNPPYPCWRYNTAVTMFENTTTISPEFFATVCDATHQEGAYPTQ
                     FSTVYDLQHQIIYLYWQHNYNQVKIYNLTEELQSGYHIYSIPTLFNTSQPPNPPTITG
                     PTSGNKGEPVQYNFNSTDPDVDDVCYFIDWGDTTNSGWIGPNASGEAITISHTWNTRG
                     VYTIKAKAKDINGWESDWATLKVRMPLAYNIPFLSFWQQLFERFTHIFPILRYLLEE"
     gene            3845..4057
                     /locus_tag="VN377_04270"
     CDS             3845..4057
                     /locus_tag="VN377_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27634.1"
                     /translation="MMKKHLNLLIEAGLLQKARDNGLVISKFLEIKSQGYFPFMDIVS
                     KPQVYSQSDSVGPLRFTVSYRRESGR"
     gene            complement(4186..>4399)
                     /locus_tag="VN377_04275"
     CDS             complement(4186..>4399)
                     /locus_tag="VN377_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27635.1"
                     /translation="TKTILPSVARSTCIGPYFFVASVIKRIILGKKYKPENDDYFYMG
                     AVVVGDMLGLAKILHFGSIVAVKPKH"
BASE COUNT         1305 a          803 c         1010 g         1281 t
ORIGIN      
        1 cttatacgac ctgaaaaagg atacaaggtg atagacgagt aatgttcttg atttcgtatg
       61 gatagatatt ttttgttcac aaaatattgt agctttgatt agaaagtatt gttccatcgt
      121 taagcctaaa taatagcgaa tgttactcca tatactttct ttcagaaggg cttgtagctc
      181 agctaggcta gagcaatcgg ctcttaaccg attggccgag ggttcgaatc cctccaagcc
      241 cgctctcttc atttcacgat cgcatcactt ttctaaaatt atcgtttgca tattggtatt
      301 ttcgtaagaa tctaataacc cacatgcgat gtaggtacta aaaaaacaaa agaaaaatga
      361 ttgatggtgt tctctataaa aaagaaatgt cccgagtgta gtggaaaagc tgttcgcttg
      421 taccaaaaca gaaccataga tggaaaacgg aaatgggtgc ctaccgcatg gtgttgtact
      481 gaatgtcact ttctttacac ggtagcatca gataccctta tgtatcctat tggcggaaag
      541 gaatataatg tatcatacag tggaaaatgc ccgaattgtg acctgaaact tacccggttg
      601 ttccgccata aaaacccgtc acagggaaaa caagaatgga tctcgtctgc atggtactgc
      661 aaccgttgca aatatgtctg gcttgacaaa gaagaaaaat catagtggtt cgttcctaaa
      721 cagttaaata tagctatcta ttttaggggc aaggtagaca aaccagagga aaatctaatg
      781 aaacgcagtt cacgagtgtg gaaaaagcga aggaagatgc gctggaaatg gcgcaagaaa
      841 cggatgcgac gagaaaagcg gatgcggaga cagaaacagt aaatcatatc accatgggag
      901 catggtgtaa cccggcatca ttacgggctc cagttaggta tgattacaag agggtcatct
      961 gacgtatgat gaataactgg agcgatgacc tacgaagaga cccgtcgatc tgggttcaaa
     1021 tcccagtgct cccatccaag ttaaagagat aataaaagag gtagaaaaaa aatgaaacca
     1081 agcctaaaaa acatcaatgc atatacgata gtggcactca ttgttttaat cgcaggactt
     1141 ctcctctata tcacctgggg tcttcgttat aacgtctggg cggacatcgg tatctattca
     1201 ataacaatcg ttctggtcct tggtggtctg cttggggcga ttctgtccct tacgtatgaa
     1261 aggattgaag acggacaaga atagaatttt tcctttgata attgcgaaga tttatatagt
     1321 ggaatttggt aagtgattgt cgatggaaca ggaagagatg ctgagaaaat attcaggtga
     1381 atggatttta ttgttcaacg acgaagtcgt cgaccatagc gtcaaccttg aagacgtact
     1441 acgagctgct gatgagaagt tccctaccaa aaaattccct gcagacaacg tcaagatatc
     1501 aaaagtatta tctggcagta ttcatttaca ttaagatacc catgcctgaa acacctttcg
     1561 tctttgaata taaaaattat ccattaattc ctattaagtt ttccatcaga gatcgagaaa
     1621 ccccgatgat tgatgcgctg ctcgattccg gtggagattt tatcgtcatc ccctatgcaa
     1681 tagcgaaata tctggggctg aaattggtaa aagcagggtg tgtcgatacc gcagctgggg
     1741 aaacgacgct gtataagacc gtcgtcaaca tggttatttt tgacgaggat cagaaataca
     1801 cttacgagaa ccttgaggtg catgtctccg atcgaaacga cctgccggtt cttctgggga
     1861 gaagcccgat tttcgaggat cacgaaatca tctttcagaa acatcaaaac aaactgattt
     1921 tacaaccgat acaacaccag tgaaatcaat acgtctttct tttaggaacg gctgggagaa
     1981 actttaaatg agtcttacat gattctgatt cagaggaacg aattatgcca tatatatacg
     2041 aaggatggac gttatataag agggatgtca ccctcaaagg cggacggaaa caaaccattt
     2101 atttctttag caaaagatca ccgaaaagtg gtactccctg tgaattacca ccagggtatg
     2161 gtgttggcgt taacaaacgc accggcctac cgtatctgaa aaagaagtaa agttacttct
     2221 tttttttctt ttcaatctct cccttttaat cgcatgtggg gtatctagga attatctcca
     2281 taaatctcat atgctcaaga gcttttagga aaacgaggaa aaactcgtaa taggatccaa
     2341 cgggagaggt acgagtgcct atgaccggta atgaagaagc tatcactaga tatcattcgc
     2401 gtttttggtc caaaactgaa ccgtggtggg agaccggtaa aacccttggt atccatcttg
     2461 gttacaataa caaaatagca taagaacact atccattgga aagaagtgta gaactcgaag
     2521 tcaaccttta ttctgaatag agaaatggaa taaaatgaaa aggggttact cctccaatag
     2581 ataacgtagt attgggaaga tatgcgtgaa ccgctcgaat aattgttgcc agaatgagag
     2641 aaatgggata ttatacgcaa gcggcatacg caccttgaga gttgcccagt cactttccca
     2701 tccgttgatg tctttcgctt tcgctttgat tgtgtagact cctcgtgtat tccaggtgtg
     2761 ggatatggtg attgcttcac cggacgcatt tggaccgatc catccgctgt tggtagtatc
     2821 tccccagtcg ataaagtagc atacatcatc aacatcagga tctgtagaat tgaaattgta
     2881 ttggacgggt tctcctttgt ttccacttgt cggacccgtg atggtcggtg gatttggtgg
     2941 ttgagacgta ttgaataggg ttgggatgga atagatgtga taacctgatt ggagttcttc
     3001 ggtgagattg tagattttca cttggttgta gttgtgttgc caataaaggt atatgatttg
     3061 atgttggagg tcatacacag ttgagaattg ggtggggtat gctccttcct ggtgagttgc
     3121 atcacaaacg gtggcgaaaa actcaggtga tatggtcgtg gtgttttcaa acatggtgac
     3181 agcggtgttg tatcgccagc aaggatatgg tggattggtt tgtgagagtc tgaagttggt
     3241 caccacttga taataatctt gtttgatgtg catggggtcc ccttcgatga tgactgatgc
     3301 accggttgca tcggagacca tgagctgcat ggtttctaaa aaatcaaggt tgtactgatc
     3361 aaagacttct aggacttctt caacagttga acattgttcc atgcagatgt acatgagata
     3421 tcgtgggggg tgtgggttct gcgttgagtt ctgtacctca agatatgagg tcgatgctga
     3481 tgcgaagaag agtccttggt cgtttatccc ggtttgccac cagaattgtt gccaaccagc
     3541 atacattcgt ccatgtttac cgttttccgc cgggtaaaaa acaatgtagg tgtcatcatc
     3601 ggaataatca ccgttgttac cagcaagtgt catgatttca tcttgcgcac tgaaaatagt
     3661 acatgcaagt gttgaagttt gaagaattat catgatgatg gctgctgtca tgagtggtaa
     3721 gattttcctt tttgttttca tatgaaggaa gagttatttt gtttatatta acattaggat
     3781 tggatttttg ggtaagatct agaaaaatat catgtaatca ggtcattcgt atgaaaatga
     3841 tgttatgatg aagaaacatc tgaatctact cattgaagct ggtttgttac aaaaagcaag
     3901 agataatggc ctagtcatca gcaagtttct tgagataaaa tctcaggggt atttcccgtt
     3961 catggatatc gttagtaagc cacaagtgta ttctcaaagt gattctgtgg gtccgttaag
     4021 atttacagtg tcctacagga gagaatctgg tcggtaaaat ggcgtttttg ctcctatcga
     4081 ttctgagggc tattcgaata ttactctctt aaaacttctt cttttctttt tctcgtaaat
     4141 ggtgattgct gtggttttga ttatgaatat cgtaagggga ttttctcaat gcttaggttt
     4201 tacagcaacg atggaaccga aatgaagaat tttagctaat cccagcatgt ctcctactac
     4261 tacagccccc atgtagaaat aatcatcatt ttctggtttg tattttttcc caaggattat
     4321 tcgttttatt actgaggcga cgaaaaagta cggcccaata cacgtggatc ttgcaacaga
     4381 aggcaagata gtcttagta
//
LOCUS       DATMXJ010000163         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_3220, whole genome shotgun sequence.
ACCESSION   DATMXJ010000163 DATMXJ010000000
VERSION     DATMXJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..284)
                     /locus_tag="VN377_04280"
     CDS             complement(42..284)
                     /locus_tag="VN377_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWR27636.1"
                     /translation="MKMKIEILGAGCAKCKATEKNVRKAVDELGIQAEIVKIDDLQEI
                     VNRGVMMTPAVFIDGQAKIVGHVPRPNEIKKLLQKK"
     gene            complement(292..1254)
                     /locus_tag="VN377_04285"
     CDS             complement(292..1254)
                     /locus_tag="VN377_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWR27637.1"
                     /translation="MFYPIQLLADWLTYSVFSVPKGSLLANAVNFFIYDVIKIFILLS
                     LIIFVVSLIRTYLPPEKVRKILSRQNEYVGNVLASFLGVITPFCSCSAVPLFLGFVEV
                     GVPLGMTFSFLVASPMINEVALVLLFGMFGWEIALIYMISGILIAIFAGILIGKLKVE
                     YLVEDFVFKNKTNADLEIKEMTFKERIDYAKTYVFDILKRVWIYVIIGIGIGAWIHGY
                     VPMDFLAQFAGADKWYAVPLATLIGIPLYSNAAGIIPLVSALTEKGVAMGTTLAFMMA
                     VTGLSLPEFLILRKVMKVRLILIFAAIVGLGIIFTGYLFNFIMG"
     gene            complement(1262..>2205)
                     /locus_tag="VN377_04290"
     CDS             complement(1262..>2205)
                     /locus_tag="VN377_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HWR27638.1"
                     /translation="YFPDFSQNLGIGIPIGLFLMIYPAMTKIELGELKAALKSKRQVG
                     IIVFFNYAVNPFLLYFLGLLFFKMILPYFGLIDPATANYLWTGLILLGVAPCIAMVLV
                     WTDLAKGNGPLGIVLMAWNSIIQVITTPFYIALLVGTYVAIDIMLIGQSVLLYLGLPL
                     LFGVITRRLVIKRKSERWLNEKLIPYFNSMQLLALLFTMVVMFSLKGGVILDNPNLIW
                     QMAIPVVLFFFLLYNIVYHTCRRLGYNYSDSSTMGFHCTGRNFELAIAIALTAFATMP
                     MVAVSTVVGPLIEIPVMLTIVWMALRTRKKLLRTKGA"
BASE COUNT          645 a          517 c          463 g          580 t
ORIGIN      
        1 catctgcctc acagagacat ttattttttt tcatcagtca ttcacttctt ttggagcagc
       61 tttttaatct cgttaggcct tggaacgtgt ccaacaatct tggcttgtcc gtcgataaaa
      121 accgcaggag tcatcattac gcctctgttt acaatctcct ggagatcgtc tattttgacg
      181 atctctgctt gtatccctag ttcatcgaca gcttttctca cgtttttctc ggttgccttg
      241 catttcgcac agcccgcgcc taagatttcg attttcattt tcattacctc tctagcccat
      301 gataaaatta aacaaatatc ctgtgaagat gatcccgagt cctacgatcg ctgcaaatat
      361 gaggatgagt ctgaccttca tcactttcct caaaatcaag aactctggca atgataatcc
      421 ggtgaccgcc atcataaatg caagagtagt ccccatagct actccttttt cagttaatgc
      481 actcaccaaa ggtattattc ctgctgcatt ggaatacagg ggaatgccga taagtgttgc
      541 cagaggcaca gcgtaccatt tatccgcacc agcaaattgc gcaaggaaat ccatgggaac
      601 ataaccatgg atccatgctc ctataccaat gccgataatg acgtaaatcc atactctttt
      661 taagatatca aaaacatatg tttttgcgta gtcaatccgt tccttaaaag tcatctcttt
      721 tatttccaga tctgcattcg tcttattctt aaagacaaag tcttcgacca gatactccac
      781 ctttaatttt ccaatgagga ttccagcaaa tattgctatg agtatcccgc ttatcatgta
      841 aatcagggcg atctcccagc caaacatacc aaacagtaag actaaggcaa cctcattgat
      901 catcggagac gcaacaagga acgaaaatgt catacccaac ggcaccccta cttcaacaaa
      961 tcccaggaaa agagggactg cactgcagga acaaaaggga gtaataactc caagaaatga
     1021 agcgagaaca ttaccaacat attcattctg acgagataga atctttcgta ccttctccgg
     1081 aggaagatag gttcttatga gcgataccac aaatatgatg agtgaaagaa gtatgaatat
     1141 cttgatgaca tcatagatga aaaaattcac tgcgtttgcc aacaatgaac cctttggtac
     1201 ggaaaatact gaatacgtaa gccagtctgc gagtaattgg atcggataaa atatatccat
     1261 attacgctcc tttagtacgt agcagctttt ttcttgttct cagcgccatc catactatcg
     1321 taagcatgac aggaatctcg atgagaggtc cgaccacggt agacacagca accataggca
     1381 tcgtcgcaaa agcagtcaat gctattgcta tcgctaactc aaagtttctt cctgtgcagt
     1441 gaaatcccat cgtggaagag tcgctataat tatagcctaa tcgtcggcag gtatgataaa
     1501 cgatgttgta aaggaggaag aagaacagta caaccgggat ggccatctgc cagataagat
     1561 tcgggttatc gaggatgacc ccgcccttta gggagaacat gacgaccatt gtgaagagca
     1621 atgcgagaag ctgcattgaa ttgaaatacg ggatgagttt ttcgttaagc catctctccg
     1681 acttgcgctt gatcacgagc cgtctggtga tgacgccgaa gagaagcggc agccctaaat
     1741 acaagaggac actctggcca atcagcatga tgtcgatggc cacgtatgtt cctacaagaa
     1801 gcgcgatgta gaacggggtt gttatcactt ggatgatgga attccaggcc atgagcacta
     1861 tcccaagcgg gccgtttccc ttcgcaaggt ctgtccagac cagcaccatc gctatgcacg
     1921 gtgcgacgcc cagcagtatc aggccagtcc agagataatt tgccgttgcg gggtctatca
     1981 atccgaaata ggggagaatc atcttgaaaa atagcagacc cagaaagtac aaaaggaacg
     2041 gattgaccgc gtagttgaag aatacgatga taccgacttg tctcttgctc ttcagggctg
     2101 ccttgagttc gccaagttcg atctttgtca tcgctgggta gatcatcagg aacagtccga
     2161 tggggatacc tatccctagg ttctggctga aatctggaaa atatt
//
LOCUS       DATMXJ010000164         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_66148, whole genome shotgun sequence.
ACCESSION   DATMXJ010000164 DATMXJ010000000
VERSION     DATMXJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66148"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3939)
                     /locus_tag="VN377_04295"
     CDS             complement(<1..3939)
                     /locus_tag="VN377_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27639.1"
                     /translation="MFFIGNITIVLSQQTELLKTPDTGSNKPKILETDSSGFAINYTM
                     TFSKNDFSLNTLQGFDTVQLYDGGYTTEIGKPMIPVKNIRMAIPAEMKVSTIEILDIK
                     EEPLDGTYLIFPTQTPLPVDSTLKENQFIKPDYQTYISHQPYPSKLIEFIGQSDLAGQ
                     VMVDITIYPIHYIPNEKKLTLVTSITFNIIGTKGYVCGDYLSTRISECRQNLCLRSVQ
                     NMVVNPEAVALQFSLTPQPAGVNPGDYDYVIITQDSWVSAFQPLADWKTKKGIPTNIV
                     TTSWIYNSGGYSGSNVDKIRAFVLDVYTNWGTIYVLLGGDVDVVPCHYRTFSQVDPDL
                     VPNDVYYADYDGDWICEVNVGRASVTGPGSGTGQIGNFINKVLTYETNPPLTNYAKNA
                     AFFGFDLDSTTYGEQCKINIDNTYIPSSWSMTTVYDSQSGNHRTNVIAALNVGPNLVN
                     HVDHCNSGYLGTGYVNHNLGLSNYDMDALTNGNKQTIMYSQGCDPAAFDVSNCIAEHF
                     VRDNNGGGIAFIGNSRYGWYNYGQYNTLSMRYDVNFFKSLFQENLYHLGAAFTDHKND
                     GIQSQPGNELYRYLFTGLTLLGDPELPIWTENPTSLSVTHPDQLLRDSSSFTITVTSN
                     GNPVSQSYVCLWKENEVYLTGYTNTNGVITFHPSPTTIGTMFITVTKQNYLPYEGSAT
                     VVNQPPISDFTYSPINPITTDTIQFTDTSTDSDGTVISWSWTFGDGTTSNIRNPQHQY
                     ADDGTYLVVLNVIDNDGAENTRTKSINVSNVPPFVNFTHSPFYPLTSDTILFNDTSTD
                     IDGTIISWLWRFGDGSSSTLQSPSHKYDDNGVYTVTLNVTDDDDAFESKSISISVSNV
                     PPSPDFNYLPSNPITTDTIQFTDTSTDSDGTIVSWFWRFGDGSTSAQQNPLYKYSDNG
                     DYTIILNITDDDGAKNAKTKSISVSNAPPAANFSFEPINPTTQETVFFNSTSYDSDGV
                     LSNYTWDFVDGNFSYEQKTTHMYSDNGTYLITLNVTDNDGDTDTIQKTIQVSPNYPPN
                     TPNDPSPTNDASNIPLFSTLSWTSGDPNMDSLTYDVYFGMTSPPDKVKSNQSDTTYDP
                     GTMNFTITYYWKVVAWDTHGVNATSPTWHFTTRTNHPPVAVPDSFSTNEDTTLSVSAP
                     GVLGNDEESDNDIMTAIKVSNTIHGTVTLNSNGSFSYIPIANYYGIDTFTYKAYDGFV
                     YSPVENVTITIHSVNDPPVLGTSSPGNGSTVNKLSLTWSIPITDIEGDLFSWTIQCNN
                     GQTNISTGANNGTKTLFLSGLAYSTTYKIWVNATDPTGSDQFIRKWYTITP"
     gene            4368..>4972
                     /locus_tag="VN377_04300"
     CDS             4368..>4972
                     /locus_tag="VN377_04300"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HWR27640.1"
                     /translation="MKNIWMSILVIGVTAGLLGTGTYAYFSDVETAKNTFSAGTLDLR
                     LSADGVNYYNDPIDPLIVCTNMAPGDESVEKTLYFKNVGTIGGIVTVDISYVEHDDPL
                     DADAFYEYSVANGGTDVSADDFAVHLWITESSLDGGPNIVYYWALQIVDDSYGSDWAA
                     AISAGAVYDPDGGVGVPDGDELPTAYGLQSVVLHFWDTYHGT"
BASE COUNT         1347 a          910 c         1130 g         1585 t
ORIGIN      
        1 gggtgtgata gtgtaccatt ttctaataaa ttgatcgcta ccagttgggt cagtcgcgtt
       61 cacccagatc ttgtaagtcg ttgaatatgc aagtccggaa aggaagagtg tttttgttcc
      121 attgtttgca ccagtactga tgttcgtttg cccgttgtta cattggatgg tccaggagaa
      181 aagatctcct tcaatatcag tgatgggaat gctccaagtc agactcaatt tattcactgt
      241 agaaccgttc cctggagaag atgttccaag cactggtgga tcgttcactg aatgtatcgt
      301 aatcgtcacg ttttccacag gagagtaaac gaatccatca taagccttgt aggtgaacgt
      361 atcaataccg taataattcg cgattggaat ataactgaag cttccgttac tgttcaacgt
      421 gaccgtacca tgaatggtgt tagatacctt tatcgcagtc attatatcgt tatcagattc
      481 ttcatcgttt ccaagtacgc caggtgctga tactgaaaga gtggtgtcct catttgtgct
      541 aaaactgtct gggaccgcaa cggggggatg atttgtccga gtagtaaagt gccaggttgg
      601 gcttgttgca tttactccat gtgtatccca tgcgacaacc ttccaataat aggttatcgt
      661 gaagttcatt gttccgggat cgtaagttgt atcagattga ttactcttaa ctttgtctgg
      721 cggacttgtc atgccaaagt acacatcata ggtcaatgaa tccatattag gatcaccact
      781 agtccaactc agagtagaaa acaatggaat attacttgca tcatttgttg gggatggatc
      841 atttggagta tttggtggat aatttggtga gacttggatg gttttctgaa tcgtatcagt
      901 atcgccatca ttatctgtta cattcaacgt gattagatat gtcccattat ctgagtacat
      961 gtgtgttgtt ttttgttcat aactgaagtt tccatctacg aaatcccagg tgtaattcga
     1021 gagtactcca tcggaatcat aggaggtact attgaaaaaa actgtctctt gtgttgttgg
     1081 gttgattggt tcaaaactga aattggcagc tggtggggca ttactcacag atattgattt
     1141 tgttttagcg ttttttgctc catcatcatc agtgatattt agaatgattg tataatctcc
     1201 gttatctgaa tacttataga gtggattctg ttgagccgat gtgctaccat caccgaatct
     1261 ccagaaccat gagacaatcg ttccgtctga atcagtggat gtatccgtga actgaatagt
     1321 atcggttgtt attggattcg atggaagata gttgaagtca ggcgatggtg gaacattact
     1381 cactgaaatt gagatggatt tcgattcaaa cgcatcatcg tcatcagtca cgtttaacgt
     1441 cacggtgtag acaccattgt catcatattt atgacttgga ctttgaaggg tagaagaact
     1501 cccatccccg aacctccata accaggagat aattgttcca tcaatgtctg ttgaggtatc
     1561 attgaaaagg attgtatcag aagtcaatgg atagaatgga gaatgggtaa aattaacaaa
     1621 tggaggtaca ttgcttacgt taattgattt agtccttgta ttttctgctc catcattatc
     1681 gataacgttg agtacgacga gatatgttcc atcatcagca tattggtgtt gtggatttct
     1741 aatattggat gtagttccat ccccgaaagt ccatgaccag gatatgacag ttccatctga
     1801 atcagtggat gtatccgtga attggatagt atcagttgtt attggattaa tcggagaata
     1861 agtaaagtca gaaataggag gttgattcac aacagttgct gaaccctcat aaggtagata
     1921 attttgtttg gttacagtaa taaacattgt tccgatggtg gtcggtgagg gatggaaagt
     1981 aattacacca ttggtgtttg tataaccagt taaatagacc tcgttttctt tccagaggca
     2041 gacataggat tgactgacgg ggttaccatt tgaggttacg gtaatcgtaa atgatgagga
     2101 gtcacgaagt aactgatctg ggtgggtgac acttaaactt gttggatttt ccgtccatat
     2161 aggtaactca ggatcaccaa gcaaggtaag cccggtaaaa aggtatctat aaagttcatt
     2221 tcctggttga ctttgaattc cgtcattctt atgatcggtg aaagctgcac ccagatgata
     2281 gagattctcc tgaaacagtg atttgaagaa attaacatca tatctcatgg ataaggtatt
     2341 gtattgacca taattgtacc agccgtaccg tgagttaccg ataaaagcga ttccacctcc
     2401 attattgtct cgtacgaaat gctcggcgat gcaattcgag acgtcaaatg ctgcaggatc
     2461 acaaccctgg gagtacatga ttgtctgctt atttccattc gttaatgcat ccatatcata
     2521 attcgataaa ccgagattat gattcacata tcctgtaccc aagtaaccag agttacaatg
     2581 atcaacatga tttacgagat tcggtcctac gttaagagca gcaatgacgt ttgttctatg
     2641 attcccactc tggctgtcgt atacggttgt catcgaccaa cttgagggaa tgtaggtatt
     2701 atcaatattt atcttacact gttctccata ggttgtacta tctagatcaa aaccaaaaaa
     2761 agctgcattt ttcgcataat tggtaagcgg tggatttgtt tcatatgtca gtaccttgtt
     2821 aatgaaattc cctatttgtc ctgtgccact tccaggaccg gtgaccgatg cacgtccaac
     2881 gtttacttca cagatccaat caccatcata gtcagcgtaa tacacatcgt ttgggacaag
     2941 atctgggtca acctgggaaa aggtacgata atgacagggg acgacatcga catctcctcc
     3001 gagaaggacg tagatggtgc cccaattcgt gtagacatcc agaacgaacg cccgaatttt
     3061 atcgacattg gatccactgt agccaccact gttatagatc caagatgtcg taacaatatt
     3121 agttgggatg cctttttttg ttttccagtc cgcgagcggt tggaatgcgc tcacccagct
     3181 gtcctgggtg atgatgacat agtcataatc gcctgggttt acaccagcag gttgcggggt
     3241 tagtgaaaat tgaagtgcga ctgcttcagg gttaaccacc atgttctgaa ccgaccggag
     3301 acacaaattt tgtctacatt cggaaatacg tgtcgaaagg taatcaccac agacatatcc
     3361 cttagttcct atgatattga atgtaatcga agtgacaaga gttaatttct tttcatttgg
     3421 gatatagtgg ataggataaa tggtgatatc taccatgact tgaccggcaa gatcactttg
     3481 tccgataaac tctatcagtt ttgatggata tggttgatga gatatgtaag tttggtaatc
     3541 tggtttgata aattgatttt cttttaaagt gctatcaacg ggtaatggtg tttgtgtagg
     3601 aaaaataagg tatgttccat ccaatggttc ttcttttata tcaaggattt caatagtgga
     3661 taccttcatt tctgctggta tcgccatccg tatatttttt acgggaatca ttggtttacc
     3721 tatctctgtc gtgtatcctc catcatataa ttgtaccgta tcgaatcctt ggagtgtatt
     3781 caatgaaaaa tcatttttag aaaatgtcat cgtatagttt attgcaaaac cactactatc
     3841 cgtttctaat atttttggtt tattcgagcc agtatccggc gttttaagta attccgtttg
     3901 ttgtgataga acaattgtga tgtttccgat aaaaaacatc agaacgagag tatatatcat
     3961 tatttttcgc aaaacctttg cctccaattt gatattttgt aacaatcttc tcctataaaa
     4021 acatttacta ctcctttgaa gtccgatttt ttttactctt aatctttttt ttgtttccgg
     4081 caaacggaaa aaaaatggtt ggtttttggt tttttggtgg attttttctg aatggggttt
     4141 tatattttcg atgggtgttg tttgtaaacg gagttaaaac gtaggtagcg tggtgaggcg
     4201 aggagagagg acacagagac ataaccatgg atgagtcttt gtgtgctctg ttgaatcata
     4261 tccttgtggt cactgtcacc tgcttgtggg ggagagattc ggctttgcac tccgccttac
     4321 agaagaaaac aaagcagaag taaccgaaaa aggaggaaaa taaaaaaatg aagaacatat
     4381 ggatgagtat cttagtgatt ggtgtcaccg ctggtcttct tggtaccggg acgtatgcgt
     4441 atttttctga tgtggaaacc gcgaagaaca cgttttctgc tggtacgctt gatcttcgac
     4501 tgagtgctga tggggtaaac tactacaacg accctatcga cccgttgatc gtttgtacca
     4561 atatggcgcc tggtgatgaa tcagtggaaa agaccctgta tttcaagaac gttgggacta
     4621 ttggcggtat cgttaccgtt gatatctcct atgtggagca tgatgatcca ttggatgctg
     4681 atgcgttcta tgagtattca gttgcaaatg gtggaactga tgtgagtgct gatgattttg
     4741 cggtacatct gtggattacg gagtcgtctc ttgatggtgg accgaacatc gtctattact
     4801 gggcgttaca aattgttgat gactcgtatg gatcagactg ggctgctgcg atttctgctg
     4861 gagctgtgta tgacccagat ggtggcgttg gagtccctga tggtgatgag ctgccgaccg
     4921 cgtatggtct gcaatctgtc gtcttgcatt tctgggacac gtatcatgga ac
//
LOCUS       DATMXJ010000165         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_19029, whole genome shotgun sequence.
ACCESSION   DATMXJ010000165 DATMXJ010000000
VERSION     DATMXJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19029"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VN377_04305"
     CDS             complement(<1..497)
                     /locus_tag="VN377_04305"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWR27641.1"
                     /translation="MTESIKSKIRTIPDWPKKNIMFRDITTLLKSPDGLKETVDLLYN
                     RYADKKIDKVIGIESRGFILAAPLAYRLGCGFIPVRKPGKLPAECESEEYSLEYGKDK
                     IEIHKDAIDKGDKVLIVDDLLATGGTMNAARNLVKKLEGEIVECAFVVELPDLKGREK
                     IKGEN"
     gene            587..1447
                     /gene="mtnP"
                     /locus_tag="VN377_04310"
     CDS             587..1447
                     /gene="mtnP"
                     /locus_tag="VN377_04310"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWR27642.1"
                     /translation="MSVKIGIIGGSGLYKMGIIDDVQELSVDTPFGKPSDKITLGKID
                     GIEIAFLPRHGKGHTLAPHMVNYRANVFALKELGVEYLVSTAAVGSLKKELKPCDFVI
                     ADQLIDRSFKRSTTFFENGIVAHVGFADPFCKTLSDLAFDVARSEGVRVHRGSYICME
                     GPQFSTRAESKLYRSWDVDVVGMTLAPEAKLAREAEMCLCGILTVTDYDCWYEGEKDV
                     CVASVVENLKKNDTNIASIIKKLIPKIRYKRTCECHSALEYGILTSPELLKKNADPRV
                     AKVLLKRFIK"
     gene            complement(1469..1723)
                     /locus_tag="VN377_04315"
     CDS             complement(1469..1723)
                     /locus_tag="VN377_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27643.1"
                     /translation="MWQDIIIALVGLSFGFMLFPQLRDVIHGKSVNVYTAGLTTAGLY
                     ILAITFFTLDMLITVIAEIFSGTVWLLLFLFSATNKRKAK"
     gene            complement(2023..3750)
                     /locus_tag="VN377_04320"
     CDS             complement(2023..3750)
                     /locus_tag="VN377_04320"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HWR27644.1"
                     /translation="MNHKNYTKIIVLITTALFTATLFNTGALSQNKESLMPPLPLASK
                     EGHFLYQPWHSTTAYLINETGVVNHTWQGDYYPLYDSYMDHDGSVYFTIDSGRGGVQV
                     VAYNGSLLWDYRYARDSSYATHDIAILPNGDFLIIMQEIKSAAQAIQKGRNPSTIWFG
                     NFNPNFVIQVHQTGPTSGDIVWEWHIWDHMIQDFDPTKDNYGVIADHPELADINCDYW
                     WNVGWPYMNSIDYNPETDQILLSAHNCDEIWIIDHSTTTEEATGHTGGIYGHGGDLIY
                     RWGNPQAYDRGTAADEKLYLQHQVCWVKPGLPHAGNILVFSNGNNRPGGYLSSCDEFT
                     PDIDNATGEYYQPPAGEPYGPANLSWQFMLPQNLFSDGFGGAQRMLNGDTLICSGWNG
                     VFIRVTPDKQIVWTFNNPYPYMNDEVYRTQYIPPYEPPPPQPPQLPDLECNGSLSWSN
                     IKPGSTVHGSFQVMNIGDNDSLLNWTVNNTPTWGTWSFTPSSSENLTPAQGPVTVQVY
                     VVVPKEKNAGFQGNITIVNKENSSDYCMIPVTLTTPLSQNIEHHGFLARLLVRFPNAF
                     PLFRYLMGF"
     gene            complement(4016..>4638)
                     /locus_tag="VN377_04325"
     CDS             complement(4016..>4638)
                     /locus_tag="VN377_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27645.1"
                     /translation="FIIAGAMNYLGCLLKTDANGNETWRKTPFLTDATFFGYAAKQTT
                     DGGYIATGSGLVKTDSSGNEAWNITLPTPFTGTQQTNDGGYIIAGSTTGYYNGDVWLI
                     KFGPEPETPKLSFTVTGGLGVHVKIKNNGTANATGVTWQVHVEGGILHRLNQTVNGTI
                     DVAAGETKTMKTNMLFGLGAIQVTVMVGDETKIVEGTQVLILSLLK"
BASE COUNT         1140 a          985 c         1186 g         1327 t
ORIGIN      
        1 atattttctc ctttgatttt ttctcttcct tttaaatctg gcaactctac gacaaatgca
       61 cattcaacaa tctcgccttc taattttttc acaagattcc ttgctgcatt cattgttccg
      121 ccagtcgcaa gcagatcatc aacgattaaa accttgtccc ctttgtcaat tgcatcctta
      181 tgtatctcta ttttgtcttt cccatattct aatgaatatt cttcagattc gcattctgca
      241 ggtagttttc ccggttttcg aactggtata aaaccgcatc ctaagcgata cgcaaggggg
      301 gcagctaaaa taaaaccacg ggattcaatg ccgatgactt tgtcaatttt cttatcagca
      361 tatctgttat acaaaaggtc aacagtttct ttcagtccgt ctggactctt taaaagagtc
      421 gtaatatccc tgaacatgat attctttttt ggccaatcag ggattgttcg gatttttgat
      481 tttattgatt ccgtcatttt ttttctatcg tcttgtatgt agttcttcta catatacttt
      541 aacattcata gaaacctttt tttagggttt tctgttcaga agacgtatgt cggtaaaaat
      601 aggaattatc ggtggaagcg gactgtataa aatgggtatc attgatgatg tgcaggagct
      661 ttcggttgat acgccgtttg gaaaaccatc agataaaatt actctgggga agatcgatgg
      721 tattgagata gcgtttttac cacgtcatgg aaaaggtcat acgcttgcgc ctcatatggt
      781 gaattatcga gcaaatgttt ttgcgttgaa agaacttggt gtagagtatc tggtgtcgac
      841 tgctgcggtt gggagtctga aaaaagagtt aaaaccctgt gattttgtaa ttgctgatca
      901 attaattgat cggagtttta aacgaagtac aacgtttttt gaaaatggga ttgtcgctca
      961 tgttggtttt gctgacccgt tttgtaagac gctctctgat cttgcgtttg atgttgcccg
     1021 gagtgaagga gtccgtgttc atcgtgggtc gtatatttgt atggagggtc cccagttttc
     1081 aacccgggct gagtccaagc tctatcgaag ttgggatgtt gatgtggtgg ggatgacgct
     1141 tgcgcctgag gcaaaacttg ctcgtgaagc agagatgtgc ttatgtggga ttctcacggt
     1201 gactgattac gattgttggt atgagggtga aaaagatgtg tgtgttgcct cggttgttga
     1261 gaatctgaaa aagaatgata cgaacatcgc cagcattatt aaaaaattga ttccgaagat
     1321 acggtataag agaacatgtg aatgtcattc tgcgttggag tacggtattt tgacgtcacc
     1381 ggagctgttg aagaagaatg ctgatccgcg ggttgccaag gtacttctca agagatttat
     1441 aaaataaata aaaccagggg gggcgagtct attttgcttt tcttttgttt gttgcagaga
     1501 agaggaacaa taaaagccaa accgtacctg aaaaaatctc tgcaataact gtgatcaaca
     1561 tatcaagggt aaaaaacgtt atcgccagga tgtacaatcc agctgttgtc agccccgcgg
     1621 tatacacatt caccgatttg ccatgaataa cgtctcgtaa ttgtgggaac agcataaagc
     1681 caaacgacag tccaacaaga gcaatgatga tatcctgcca catagagcaa tgggataaca
     1741 tcattatcca taaaactatt tgtaacacaa atcaagaagc aggaaaaaag gattgatact
     1801 aatatgagaa aaaatcatac tcccgtcgtg attacgcgcc aagacttcag agcccccagg
     1861 acaggtgacg cgaagatctg acagaaaatc ctcctgggag attgtttgct agaggttcaa
     1921 tgctcctccc atattttatc gatcctaaaa aactagaggg aaagcattga cacggttctc
     1981 ccttgggtgc gaaaagcaaa aaaaagaaaa aaagtggtgg agttagaatc ccatgaggta
     2041 cctgaaaagg gggaatgcat ttggaaatcg cacaagtaat ctcgcaagga acccatgatg
     2101 ttcgatattt tggctaagcg gtgtagtgag agtgacaggg atcatgcagt aatcactact
     2161 gttttcctta ttgacgatcg tgatatttcc ttggaaccct gcgttcttct ccttaggtac
     2221 gaccacgtat acctggacgg tcactggccc ctgtgccggg gtgaggttct cactggacga
     2281 gggggtgaac gaccacgtcc cccatgttgg ggtattgttc acggtccagt tcagcagcga
     2341 gtcgttatcc ccgatgttca tcacctggaa actgccatgc acggtcgacc ctggtttgat
     2401 gttcgaccag gagaggctcc cgttgcattc gagatcaggg agctgcgggg gctgcggtgg
     2461 tggtggttca tacggtggta tgtattgggt gcggtagact tcgtcgttca tgtacggata
     2521 ggggttgttg aaggtccaga cgatttgctt gtctggcgtg acccgaatga acaccccgtt
     2581 ccatccagag cagatgaggg tatcgccgtt caacatccgc tgggcgccac caaagccatc
     2641 tgagaagagg ttttgcggca gcatgaactg ccaggagaga ttagcagggc catagggctc
     2701 cccggcaggc ggttgatagt attctcccgt cgcattgtcg atgtcagggg tgaactcatc
     2761 gcaactggaa agatatcctc ccggtcggtt gttgccattg gagaagacga ggatgtttcc
     2821 tgcatgggga agaccgggtt tgacccagca gacttggtgt tggagataca gtttctcatc
     2881 cgcagcggtt cctcgatcat acgcctgcgg gttaccccag cggtagatga gatcgccgcc
     2941 gtggccatat attcctccag tgtggcctgt ggcttcttct gtggtcgtgc tatggtcgat
     3001 aatccagatt tcgtcacagt tatgggcact gaggaggatt tggtcagttt cggggttgta
     3061 atcgatggag ttcatgtagg gccagccgac gttccaccaa tagtcgcagt tgatgtcggc
     3121 aagctctgga tgatcagcga tgacgccata gttgtctttg gtaggatcga agtcttggat
     3181 catgtgatcc cagatatgcc attcccagac gatgtcccca ctggtgggtc cagtctggtg
     3241 gacttggatg acaaaattgg ggttgaaatt gccaaaccag atagtgctag ggttgcgtcc
     3301 tttttgaatg gcttgggcgg ctgatttgat ttcttgcatg atgatgagga agtcaccgtt
     3361 ggggaggatg gcgatgtcat gggtggcata gctgctgtct cgggcatagc ggtaatccca
     3421 gaggaggctg ccgttatagg cgactacttg gactccacca cgaccagagt caatggtgaa
     3481 gtacacggag ccgtcatgat ccatgtacga gtcatataag ggataataat caccctgcca
     3541 ggtatggttg acgacgccgg tctcgttgat gagataggcc gtcgttgaat gccagggctg
     3601 gtataggaag tggccttcct tgctagcaag cggcaaaggt ggcattaggc tctctttgtt
     3661 ctgtgaaaga gctccggtgt tgaaaagagt tgccgtgaaa agggcggtgg taatcagtac
     3721 tatgattttg gtataattct tatggttcat gtctcatcct catgtgaaat ctttgagtgg
     3781 gatggatatt tcgtgtttct atccagttgt caacatatac gaatgttgat ttcaggatag
     3841 gaatcccctt aatctagtaa ggctcccggg gctatataaa aatatgcctg aattttcatg
     3901 aaaagtcatg atattaatga atgcctctgc ctttttgcac tgctctcaaa cgatttctca
     3961 gataatttct atgagaagtt tctccgtaga agactcaggc tcttttgcta ccatttcact
     4021 tcaagaggga gaggatgagc acttgtgtgc cctcgacaat cttggtctca tcgccgacca
     4081 tcacggtcac ctggatggcc ccaagaccga acaacatgtt cgtcttcatc gtcttcgttt
     4141 ctccagcagc gacgtcgatg gtcccattca cggtttggtt cagtcggtga aggatgccac
     4201 cttcgacatg aacctgccaa gtgacaccgg tcgcgttcgc agtcccattg tttttgatct
     4261 tcacatggac gccaaggcca cccgtgactg tgaatgagag tttgggcgtc tcaggctctg
     4321 gtccgaattt tatcaaccat acgtcaccat tgtagtatcc ggtggtcgac ccggcgatga
     4381 tatagccgcc gtcgtttgtc tgctgtgttc cggtgaacgg tgtgggcagc gtgatattcc
     4441 atgcctcgtt cccagagcta tcggtcttga ccagcccgct accggtcgcg atgtacccac
     4501 catcagtggt ttgttttgct gcgtagccga agaacgtggc gtcagttaga aacggggttt
     4561 tcctccatgt ctcgttgccg tttgcatcgg tcttcaacaa acaccccagg taattcatcg
     4621 ctcctgcgat gataaagc
//
LOCUS       DATMXJ010000166         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_45329, whole genome shotgun sequence.
ACCESSION   DATMXJ010000166 DATMXJ010000000
VERSION     DATMXJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45329"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="VN377_04330"
     CDS             <1..1929
                     /locus_tag="VN377_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWR27646.1"
                     /translation="ESWFIAMSKVQESLVKNNNQIAWYPSHLQQGRFGDFIRDVKDWA
                     LSRDRYWGTPLPIWTCTNKECHHTICVGSVQELRDLSKSFPEKYDLHKPFVDELFVQC
                     PKCHERMVREKEVIDCWYDSGSAFFAQWHYPFENQELFKENYPIDFISEALDQTRGWF
                     YSLLAISTFLFDKNPYKTVLTLGLVLDKDNQKMSKSKKNYVDPTIILDHEGADALRWY
                     LISANAPWMSTRFYEDAVKDTLGKFILTYWNSYNFFTTYAILDAFDAKNGYVPLSERG
                     LLDRWIMSRFNNLTRVVQGYMKTFETHKAARAIEDFVIDDFSNWYLRRSRKRLWSEMM
                     TPDKLSGYHTMYEIFIGLSQLLAPFIPFITEEMYQNLKTAEMPESVHLCSYPTLDKNA
                     IHEDLEKGMEQIRALVESGRALRSRINIKGRHPLPTASIVCSKEIEKSTKPLLDLIKE
                     ELNVKNVEYARDSTAFLTKTVKPKYSHLGPKYKEKAKHITQALETQDTHLLYEQLQKK
                     KEVILSVDGEKIHLTSDDFEIVEHEKEQFAKATIQDITLFLDTTITPALEAEGLAREL
                     IRRIQSMRKEVNLAVEDRIVTEIALDTTKQAALEAWKDHIKEETRSKSVSFVDTPTGI
                     LRKKWMIDDLSVDISIRK"
     gene            1998..>2848
                     /locus_tag="VN377_04335"
     CDS             1998..>2848
                     /locus_tag="VN377_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27647.1"
                     /translation="MQRKTARNIVSGKKNLCQSFFKMIPVFCIISLLSVTIFFASPVV
                     FSKTPMFIPSTPLGTTFGYVGIDYEYAIVTLNPDSFWLFDWGDGTNSSWLQLEQNQTS
                     ITETHHWDAVGTYQLHVKYRSETAPYGIWSDAMMVEIAMYSNDDFPYTPSLYTGKIQG
                     FNSTLYTYSALTTDPYDYQVCYRFDYGNGTFSDWTPYVPSGTSSYLSFAWEKPGVYVL
                     RTQAKNQYGLESAWSDPVQIIITDKAAGNGTSIDLLVLNNITYQIIYTSAYEGTLYNP
                     STGASND"
BASE COUNT          864 a          601 c          609 g          774 t
ORIGIN      
        1 gagtcttggt ttatagcgat gtcaaaagtc caagagtcac tggtgaaaaa caacaatcag
       61 atcgcctggt atccatctca tcttcaacaa ggacgattcg gtgatttcat tcgagatgtc
      121 aaagactggg ctctttcacg ggaccgatac tggggaacac ccctgccgat ttggacgtgt
      181 acaaacaagg aatgccatca tacaatctgt gtcggtagtg tccaagagct tcgagaccta
      241 agcaagagtt ttcctgagaa atatgatctt cacaaaccct ttgtcgatga gcttttcgtt
      301 caatgtccga aatgccatga acggatggtg cgggaaaaag aagtcattga ctgctggtat
      361 gattccggtt cagcgttttt tgctcaatgg cattatccgt ttgaaaatca ggaacttttc
      421 aaagaaaatt acccgatcga tttcataagc gaggctttgg atcagacaag aggatggttc
      481 tactcgttac tcgctatctc cacgttttta tttgacaaaa acccatacaa aaccgttttg
      541 acccttgggc tagtgcttga taaagacaac cagaagatga gcaaaagcaa aaagaactat
      601 gtcgacccaa ccatcatcct tgaccatgaa ggcgcagatg cgttacgatg gtacctcatt
      661 tccgcgaacg cgccatggat gtccacccgg ttctatgaag atgcggtaaa agacacgctt
      721 ggaaagttca tactaaccta ttggaactcg tataatttct tcaccaccta cgcgatatta
      781 gatgcttttg atgcaaagaa cggttatgtc cctctctcgg aacgaggact tcttgatcga
      841 tggatcatga gtcgtttcaa caacctcacc agagttgtcc aagggtatat gaaaacattt
      901 gagacccata aagctgctcg tgcgattgag gattttgtga ttgatgattt cagtaactgg
      961 tacctgcggc ggtctcgaaa aagactatgg agcgagatga tgacccctga taagctctct
     1021 gggtatcaca cgatgtatga aatctttatt ggattatctc aattgcttgc cccatttatc
     1081 ccgtttatca ctgaggagat gtatcaaaat cttaagacag ctgagatgcc tgaaagtgtc
     1141 catctctgca gttatcccac tcttgacaag aacgcgatac atgaggatct tgaaaaaggt
     1201 atggaacaga tacgagcatt ggtcgaatct ggtcgtgccc ttcgatcgag aatcaacatc
     1261 aaaggacgac acccattacc taccgcatcc atcgtatgct ccaaagaaat tgaaaaatca
     1321 acaaaaccat tacttgactt gataaaagaa gaactcaatg tgaaaaacgt tgagtacgca
     1381 agggattcga ctgcatttct tacgaaaacc gttaaaccaa aatactcgca tcttggtcca
     1441 aaatacaaag aaaaagcaaa gcacatcacc caggctcttg aaacgcaaga tactcacctc
     1501 ctctatgaac aactccagaa gaaaaaagaa gtgattcttt cagttgatgg tgagaagatc
     1561 catttgacct cagatgattt tgagatcgtg gaacatgaaa aagaacaatt tgcaaaggca
     1621 accattcaag atatcacctt gtttttagat acaacgatta cccctgcgct tgaagcggaa
     1681 ggattagcac gtgagctcat tcggcgcatc caatcgatgc ggaaggaagt caatcttgct
     1741 gtcgaagatc ggattgtcac tgaaatcgcg ttagacacga caaaacaagc tgccttggaa
     1801 gcttggaagg atcacatcaa agaagaaaca cgatcgaaat cagtgtcgtt tgttgataca
     1861 ccaactggga ttttacggaa aaaatggatg attgatgatc tctcagttga tatcagcata
     1921 cgaaaataga acattgcacc ggcgcacatg ccaataaagc ttatatacct agaacctgat
     1981 ttttatgaga cgaagggatg cagagaaaaa cagcacgtaa catcgttagt ggtaaaaaga
     2041 acttgtgtca gtcattcttt aaaatgattc cagttttttg tatcatctcc ctgttgagtg
     2101 tcacgatatt ctttgcttca ccagttgttt tttctaaaac ccctatgttc attccctcta
     2161 cacctttggg gacaacattt ggatatgttg ggattgatta tgagtacgcg attgtgacgt
     2221 tgaatcctga ttcgttctgg ttatttgact ggggggatgg aacgaatagc tcctggttgc
     2281 aactggaaca aaatcaaacc agtatcacag aaacacatca ctgggatgcg gtgggtacct
     2341 atcaattgca tgtgaaatat aggagcgaaa cagctccgta tggtatctgg tcagatgcga
     2401 tgatggttga gattgctatg tattctaatg atgatttccc ttacacgccc tccttgtaca
     2461 ccgggaaaat ccaggggttc aacagcacgt tatataccta ttcagcgttg acgaccgatc
     2521 catatgatta ccaagtatgt tatcgttttg attatgggaa tggtactttt tctgattgga
     2581 ccccgtatgt tccctcaggt acgagctcgt atctttcatt tgcttgggaa aaaccaggtg
     2641 tctatgtgtt acgaacccaa gcaaaaaacc aatatggtct tgaatcagct tggtctgacc
     2701 ctgttcaaat tataattacg gataaagcag cagggaatgg gacatccatc gatctccttg
     2761 ttctgaataa tatcacgtat cagattattt acacgtcagc gtatgaggga accctctata
     2821 atccttcaac aggggcatcc aatgatat
//
LOCUS       DATMXJ010000167         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_61032, whole genome shotgun sequence.
ACCESSION   DATMXJ010000167 DATMXJ010000000
VERSION     DATMXJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61032"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VN377_04340"
     CDS             <1..911
                     /locus_tag="VN377_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWR27648.1"
                     /translation="AEEKLVELHPEQMMKTPFHLYIGQEAIATGVCAALTKQDVVFSN
                     HRSHGHYLAKGGDLNRFMAEMYCKIDGCSCGKGGSMHLIDTSVGHMGSSSIVSGSIPI
                     AVGAALAFKMQNKKNVAITFFGDGAADEGVLYESYNFAALKKLPVIFICENNLYAINS
                     KIATRQAVDNLPGRAISFGVPGEKIDGNDVLKVYKTTEKMVKKAKQGQGPGFIEAVSY
                     RWKAHIGVTEDVGEGIRPAEELAEWKKKCPITTYEKYLLKEGIMTNHQLEQITEKVQC
                     LVNDAVEFGRKSPLPLAGDLLEDVFV"
     gene            908..1891
                     /locus_tag="VN377_04345"
     CDS             908..1891
                     /locus_tag="VN377_04345"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HWR27649.1"
                     /translation="MRKLSAKDAIREAILQSMQVDPSVFVYGEGVDDAHGIFGTSLDL
                     AKVFGEERVFDMPMSENAMTGVGVGAAVMGMHPIMVHQRIDFTLLALDQIVNHAAKMR
                     YMWGGHIHVPFVIRAIIGRGWGQGAQHSQSFYSIFTHFPGVKVILPSNPYDAKGLLVQ
                     AIRDGNPVISVEHKLVLFLEGDVPEEIYAIPFGKGRVVREGTDVTIVAFSQMVSEAEK
                     AAQELAVIGINAEVIDPRTAYPLDENIIAQSVKKTGKLVIADIDWVFAGMTAEIAAKI
                     AENHFDSLKAPIRRIGLPHTTHPSSYALEEVFYPTKNNIINAVKDIINYKQ"
     gene            1905..2717
                     /locus_tag="VN377_04350"
     CDS             1905..2717
                     /locus_tag="VN377_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR27650.1"
                     /translation="MDKKIKDRYRNLFSLEGKIAIVTGAYGHLGSAISQALAGFNATV
                     ILLGKNEQKLKTLIRQHPEFKGKSEYYISDVTDEKKFQTIVKDIITKHSNVDILVNNA
                     YEKQNEPFEELTKEAWDHAIATSLTHYVTCAQTVSGAMLKQKSGSIINIASIYAFLGV
                     DQRIFLDLGNNPPIHYTVAKGGILQITKYLATLWADKGIRVNAISPGYFPKKRPGVPE
                     RLDYIHEITQRTPMRRIGQPDEITGAVVYLASNASSFVTGQNIIVDGGWSSW"
     gene            2711..3226
                     /locus_tag="VN377_04355"
     CDS             2711..3226
                     /locus_tag="VN377_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWR27651.1"
                     /translation="MVKLHLGCGRDIKEGFVNIDHFKGEGVDVVCNLGHEKLPFDDNT
                     IDVVYANSFIEHLDCYETDFLLDELYRVCKPEAIIEFISPHYLAPASSRVWHKQRISE
                     GYFNEYAYDSDRMSLDSKPYFKISYKLTYMRYKPEGRPRFLPFLLILPCNIQFKLKVV
                     KGHEGRKKNEF"
     gene            3216..4061
                     /locus_tag="VN377_04360"
     CDS             3216..4061
                     /locus_tag="VN377_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HWR27652.1"
                     /translation="MNSNQKATKQPLIIVTGSNGYIGKSLLPLLHQYRCIGIDIKTNT
                     HSCSYICQTADLRKPESVKKIFEKYTPDIVYHFAALIQPQINEQNPELAKESHINITQ
                     NILDCISSDTHLFFLSTDKVFDGSHPHPNEESPTNPLWKYGEFKLHCEKMIQEQVKNH
                     HILRLPIMHSLGSPDSSSFIDKAFLQIIDGKTVQIYDNVFRCYVFLSDLLKILKNMID
                     DTHYGVYHVGTKMMSYYERVKNLCDAKHIEYEGLLQPVQGSANPMKQNLDTSKIRSLF
                     HIDFQ"
     gene            4155..5153
                     /locus_tag="VN377_04365"
     CDS             4155..5153
                     /locus_tag="VN377_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWR27653.1"
                     /translation="MLSKKRTLVTGGAGFIGSHIVDRLLADGHQVIVIDNYSTGRPEN
                     LQHHEKNQMLSVVEADIADFEIIEKYFRKVDWVFHIAALADIVPSIQRPSDYFQANVN
                     GTFSVLEAARKSKVKRFVYAASSSSYGIPDHYPTSETADIRPQYPYALTKYMGEELVF
                     HWGQVYKLPVVSMRFFNVYGPRARTSGTYGAVFGVFLAQKLNGKPFTVVGDGKQLRDF
                     IYVTDVVNACVMAAESDITGESFNIGSGNPQSINLLVEYLGGEVVYIPKRPGEPPQTH
                     ADITKIQKLLKWKPSVSFEKGVKIMVDHIQDWKNAPLWTPQKINKATEDWFKYLSK"
     gene            5160..>5588
                     /locus_tag="VN377_04370"
     CDS             5160..>5588
                     /locus_tag="VN377_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWR27654.1"
                     /translation="MNENKIVNLTELPDIIKKEKLKGKKIVLSHGCFDLLHPGHIRHF
                     KAAKKYGDILVVTLIPDKFVKKGPGRPVFNEHLRAESVASLADVDYVTLEKDENVAKT
                     IELLQPDFYVKGKEFSNNAEGETSTILQEEKSVKKIGGEIR"
BASE COUNT         1798 a         1036 c         1207 g         1547 t
ORIGIN      
        1 ttgctgaaga aaaactcgtt gagttgcacc ctgaacaaat gatgaaaaca ccgttccatc
       61 tttatatcgg ccaggaagca attgctacag gagtatgtgc tgcgcttacc aagcaagacg
      121 ttgtatttag caaccatcga agccatggac attaccttgc aaaaggcggt gatttgaaca
      181 gattcatggc ggagatgtat tgtaaaatcg atggttgcag ttgcggaaag ggtggatcaa
      241 tgcatttgat tgataccagc gttggacata tgggtagttc atcgattgtc agtggtagta
      301 tcccaattgc agttggagct gcactcgctt ttaaaatgca aaacaagaaa aacgttgcga
      361 ttacattttt tggtgatggc gctgcagatg agggagtact ctacgaatct tacaattttg
      421 cagctttaaa aaaactccca gtaattttta tttgtgaaaa taacctctat gcgattaact
      481 caaagattgc gaccagacag gcggtggata atcttccagg ccgggcgatt tcgttcggtg
      541 ttccggggga aaaaatcgat gggaacgatg tcctgaaagt ttacaagaca acagagaaga
      601 tggtgaagaa agcaaagcaa ggtcaaggcc ccgggtttat tgaagcagta tcgtatcgtt
      661 ggaaggcaca tattggtgtt actgaagatg ttggtgaagg gatacggccc gcagaagagc
      721 ttgcagaatg gaagaagaaa tgcccaatta ccacttatga gaaatattta ctgaaagaag
      781 gaattatgac aaaccatcaa ttggaacaaa taacagagaa ggttcaatgt cttgtcaatg
      841 acgcagtgga atttgggaga aaaagtccac ttcctcttgc aggtgatcta ctggaggacg
      901 tgtttgtatg agaaaactga gtgcaaaaga tgcgatccga gaggcaattc tacaatccat
      961 gcaggtagat ccatcagtgt ttgtctatgg agaaggtgtt gatgatgccc acggtatctt
     1021 tggaacaagt cttgatttag caaaagtgtt cggtgaggaa cgtgtgtttg acatgccgat
     1081 gtcagaaaat gcaatgaccg gcgttggtgt tggtgccgca gttatgggaa tgcatccaat
     1141 tatggtgcat cagcgaattg atttcactct tcttgcatta gaccaaatcg taaatcatgc
     1201 cgcaaaaatg agatatatgt ggggtggtca cattcatgtt cccttcgtta ttcgagcgat
     1261 tataggccgt ggatgggggc aaggtgcaca acactcccag agtttctata gtattttcac
     1321 tcatttccca ggagtgaaag tgattcttcc atcaaatccc tatgatgcga aaggactact
     1381 tgttcaagcg atacgtgacg gtaatccagt aatatctgtt gaacataaac ttgtgttatt
     1441 cctcgaaggg gatgttcctg aggaaatcta tgctattcct tttggaaaag gtagagtagt
     1501 tcgggaagga actgatgtga ctattgttgc attctctcaa atggtttcag aggctgaaaa
     1561 agcagcacag gaacttgcgg ttataggtat taatgctgag gtcattgacc caagaaccgc
     1621 atacccgctt gatgagaata tcatcgcaca atcagtgaaa aaaaccggga agcttgtcat
     1681 cgcagatatc gactgggtgt ttgcaggaat gactgcggaa attgcagcaa aaatagctga
     1741 gaatcatttc gattcactga aagcaccgat aagaaggatt ggcctgccgc ataccacgca
     1801 tccgagcagt tatgcattgg aagaagtctt ttatccaacg aaaaataata tcataaatgc
     1861 agtaaaagat atcattaatt acaagcaata gatgaaggga tttaatggat aaaaaaatca
     1921 aggatagata cagaaatctc ttttctctcg aaggcaaaat agcgattgta actggtgcat
     1981 atggacatct aggatctgct atttcacaag cattagccgg atttaatgct actgttattt
     2041 tacttgggaa aaacgaacag aagcttaaaa cattaatacg tcaacatcct gagtttaaag
     2101 gtaagtccga atattatatc tctgatgtca ccgatgaaaa aaagtttcaa accatcgtta
     2161 aggacatcat cactaaacat agtaatgttg atattttggt aaataacgca tatgaaaaac
     2221 aaaatgagcc tttcgaagag ctaacgaaag aagcctggga tcatgcaata gcaacgagcc
     2281 tcacccatta tgtcacttgt gctcaaacgg tcagcggtgc aatgttaaaa caaaagagtg
     2341 gttctattat caatattgct tctatctatg cattcttagg ggttgaccaa cggatttttt
     2401 tggatctagg taataaccct ccgattcatt acacagtggc aaagggtggt attttacaga
     2461 taacaaaata tcttgcgact ctttgggcag ataaaggaat ccgagtgaat gcaattagcc
     2521 caggttattt ccctaaaaaa cgcccagggg tgccagaacg acttgattac atccatgaaa
     2581 tcacgcaacg aactcctatg cgtcgtattg gacaaccaga tgaaataact ggtgctgttg
     2641 tctatcttgc ttcaaatgcc tcatcatttg tcacaggaca aaacatcatt gttgatggtg
     2701 gttggagttc gtggtaaaac tacatcttgg ttgtggaaga gacataaaag aaggttttgt
     2761 taatatcgat catttcaaag gtgagggggt tgatgttgtc tgtaaccttg gacatgagaa
     2821 gctgccattt gacgataaca caatcgatgt ggtatatgcc aatagtttca tagaacatct
     2881 tgattgttat gaaacagatt ttctgctcga tgagttgtac cgggtttgta aaccagaggc
     2941 gataatcgaa tttatctccc ctcattatct tgcacctgcc agttcaagag tatggcataa
     3001 acagaggatt agtgaaggat actttaacga gtatgcttac gatagcgatc gaatgagtct
     3061 tgattcgaaa ccgtatttta aaatctcata taaattaaca tatatgaggt ataaaccaga
     3121 gggaagaccc agattcctcc cctttcttct gattttgccg tgcaatattc agtttaaatt
     3181 aaaagttgta aaaggtcatg aaggtagaaa aaaaaatgaa ttctaaccag aaagcaacaa
     3241 agcaacccct gattattgta acaggatcaa acggttacat cgggaaatca ttgttgcctc
     3301 ttctccacca gtaccgatgt attggtatcg atataaaaac aaatacacac tcgtgctcgt
     3361 acatttgtca aaccgctgat ttacgtaaac cagagagtgt taagaagatt tttgagaaat
     3421 atacaccaga tattgtttat catttcgcag cgttaataca acctcaaatc aatgaacaaa
     3481 accctgaatt agcaaaagaa tcacatatta acatcacaca gaatattctg gactgtatct
     3541 caagcgatac acatcttttt ttcctctcaa cagataaagt gttcgatggt tcccatcccc
     3601 atcctaacga agaatcacca acaaatccac tctggaagta tggggaattc aagcttcatt
     3661 gcgaaaaaat gatacaggaa caggtcaaga accaccatat cctacggcta ccaatcatgc
     3721 attcccttgg cagtccagat tcatcctcat ttattgacaa agcgtttctt caaataatag
     3781 atgggaaaac agtccagatt tatgataatg tgtttcgctg ctatgtattt ctttcagatt
     3841 tactaaagat tctcaaaaac atgatagatg atactcatta tggagtgtac catgtaggta
     3901 ccaagatgat gagttattac gaacgtgtta aaaacctctg cgatgcgaaa cacattgaat
     3961 atgaaggatt acttcaaccg gtacagggat ctgcaaaccc tatgaaacaa aatctcgata
     4021 ccagtaaaat acgatcactc ttccacattg attttcagta ataattgatt gggattcctc
     4081 ttatttctcg tttaaaaata tataaacacg aatgaattac ctccttactg taacatcaaa
     4141 tgtggaatgt gagtatgtta agtaaaaaaa gaaccctagt cactggtgga gcaggattca
     4201 tcggcagcca tatcgtcgac cggcttcttg ctgatggtca tcaggtcatt gtcattgata
     4261 attacagtac gggacgccca gaaaatcttc agcatcatga aaaaaaccag atgctttcgg
     4321 ttgtagaggc tgacatcgct gattttgaaa tcatcgaaaa gtatttcagg aaggttgact
     4381 gggtgtttca tatcgctgca ctcgctgata ttgtcccttc cattcaacgt cctagtgatt
     4441 atttccaagc aaatgtcaat ggcacgttct cagttctcga ggcagcgcga aaatcaaagg
     4501 tgaagcgttt tgtttatgct gcatcatctt caagttatgg gatccctgac cattatccaa
     4561 catcggagac agctgatatc cgtccacaat atccatatgc gttaacaaaa tatatgggcg
     4621 aagaacttgt tttccattgg ggtcaggtgt acaaactgcc tgtggtgtca atgcggtttt
     4681 tcaatgttta tggaccgcgg gcacgaacta gtggcactta tggtgcagtc tttggtgtgt
     4741 ttcttgcgca gaaactcaac ggtaaacctt ttactgtcgt cggagatgga aagcaactgc
     4801 gtgattttat ttatgtcacc gatgtcgtta atgcatgtgt tatggcggcc gaatcagata
     4861 ttactggcga atccttcaat atagggagtg gaaacccaca aagtatcaat ctattggttg
     4921 agtaccttgg aggcgaggta gtatatattc caaaacgacc aggggagcca ccacagactc
     4981 atgcagacat tacaaaaatc cagaaattac taaaatggaa accatccgtt tctttcgaga
     5041 aaggtgtaaa aataatggtt gaccacatac aagactggaa aaatgctccc ctctggactc
     5101 ctcagaaaat taataaagca accgaagatt ggtttaaata cctatcaaag tgagtatcaa
     5161 tgaatgaaaa taagatagtc aatcttactg agttaccgga tattattaaa aaagaaaaac
     5221 tgaagggaaa aaaaattgtt cttagtcatg gatgctttga tttgcttcat ccagggcaca
     5281 ttcgtcattt taaggcagcg aaaaaatatg gagatatatt ggttgttact cttattcctg
     5341 ataaatttgt gaaaaaagga ccggggagac ctgtgtttaa tgagcatctc agagcagaat
     5401 ctgtcgcctc gttggcagat gttgactatg tgactctcga aaaagatgaa aatgtcgcga
     5461 aaaccatcga gctcttacaa ccagattttt atgtcaaagg aaaggaattt tccaataacg
     5521 cagaaggaga aacctcgacc atccttcaag aagaaaaatc agtgaagaaa atcggtgggg
     5581 aaatacgt
//
LOCUS       DATMXJ010000168         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_61036, whole genome shotgun sequence.
ACCESSION   DATMXJ010000168 DATMXJ010000000
VERSION     DATMXJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61036"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..895
                     /locus_tag="VN377_04375"
     CDS             224..895
                     /locus_tag="VN377_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR27655.1"
                     /translation="MGKITKKLKADDIGDMGIGAMIVFIAMVLVAGIAASVLIQTANR
                     LEIQAMQTGQETIGEVSTGLAVTSINGYRTALQENLSLMGITVQPRQGSFSIDLGQTY
                     IELSDSGKKVILMYGAEFQDTTDINGDFFTPGFYDGLSSTTFGIIVLEDADGSISAAT
                     PVINKGDQVMLTVNLDLVFSGAPLGLPTRTDVWGLIQPEEGAPGVFAFTSPASYASDL
                     VFNLY"
     gene            911..1576
                     /locus_tag="VN377_04380"
     CDS             911..1576
                     /locus_tag="VN377_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR27656.1"
                     /translation="MRNIKKMLKEDDLGDMGIGAMIVFIAMVLVAGIAASVLIQTANK
                     LEIQAMTTGQETTREVSTGLAVIDIEGQNTLGLITEMTITVRARAGSGDIDLSQTVIE
                     LTDGSVKTLLTYSPTALQFNSSIDEDGNIFNTGFWNMTNEQFGILVLQDEDSSLTASN
                     PVINRGDLVMLTINTDACFPVAGGLDPRDDIWGLVSPEQGASGIFAFRVPSSLTEQVY
                     DLY"
     gene            1591..2235
                     /locus_tag="VN377_04385"
     CDS             1591..2235
                     /locus_tag="VN377_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR27657.1"
                     /translation="MRNIKKMLKEDDLGDMGIGAMIVFIAMVLVAGIAASVLIQTANR
                     LEIQAMQTGQETTEEVATGIGIEDITGQIGAGAISNITICVSPRSGSKDIDLSNTVVE
                     LSDSTNKYLIIYNTTLACNVPAATGVFDTTAFATDASFSIIVLEDADGSCVGTNPVIN
                     RGDHVLLAISADALFGGLTERTDIWGMVIPESGAPGVFAFRTPASYPDTVYDLY"
     gene            2430..3071
                     /locus_tag="VN377_04390"
     CDS             2430..3071
                     /locus_tag="VN377_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR27658.1"
                     /translation="MSFQKLRTNLDADVGIGALIIFIAMIMVAGVTASVMIQTMNNLQ
                     NQAIKTSEETIRDISSGLQVTFVSGYVQNSTISQLAIFVSTTSASDDVDLTYAILTLS
                     DSAKKVILNYSSTCFRQSISNGLFDTLNASNLTSSQFGVLVIRDIDGSCNATKPVIDD
                     EDLVVLIVNTTTCLSGIGTRTDVIGEIYPEHGIHGVIGFTTPSAYVHSIIDLQ"
     gene            complement(3121..>3436)
                     /locus_tag="VN377_04395"
     CDS             complement(3121..>3436)
                     /locus_tag="VN377_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27659.1"
                     /translation="IYISFVVARHLNSILLSTNAGNPFEGTTIDVGAILTTVPPEVFT
                     QNFFLIFIVLLAHSFLMGLTIRSLRGSHFIVTFFYFIPFAWIVAITSIAVDIGLRGYL
                     GM"
BASE COUNT         1030 a          640 c          759 g         1007 t
ORIGIN      
        1 acataatttt attagattgt gcctctttga ctaagaaata tttttttatc tgataaattt
       61 tttttttgca ctaatacctg catatgcata tggtagtgta tctgcaaaca agctatttaa
      121 agaatttttt tatttgttag ggtacgcact ctcaaaagtt gtaatatttg acgcaacttt
      181 taataaaatg aaaaaagcaa cctataggag ggatataaac cagatgggaa aaataacgaa
      241 aaagttaaaa gctgatgata tcggggatat gggtattggt gcgatgattg tgtttatcgc
      301 aatggtactc gtagctggta tcgctgcttc cgtccttata cagacggcaa acaggctgga
      361 aatacaagcg atgcaaacag ggcaagaaac aattggtgaa gtctcaaccg gtctagcggt
      421 cacatctatt aatggttacc ggacagcttt acaggagaac cttagtctca tgggaattac
      481 agtacagccc cgccaaggat catttagtat tgatcttgga caaacctata tagaactctc
      541 agactcaggt aaaaaggtta tcctgatgta tggagctgag ttccaggata ctactgatat
      601 taatggagat ttctttaccc ctggattcta tgatggtttg agttcaacaa catttggaat
      661 tattgtcctg gaagacgcag atggttccat atcagcagca acacctgtca tcaacaaagg
      721 agatcaggtg atgttaaccg taaatctgga tttggtattt agtggcgcac cattaggcct
      781 tcccacaaga acagatgtat ggggtttaat acaacctgaa gaaggagcac ccggtgtctt
      841 tgcttttaca tcacctgcat cttatgcttc cgatcttgtg tttaatcttt actgaggagg
      901 atgagaaaaa atgagaaata ttaagaaaat gttgaaggaa gatgatcttg gtgacatggg
      961 tatcggtgcg atgattgttt ttatcgcgat ggtcttggtt gctggtattg cagcttcagt
     1021 actgatacag actgcaaata agttagaaat acaagcgatg accacgggtc aagaaacaac
     1081 acgtgaagta tcaactggtc ttgctgttat tgatatcgag gggcagaaca cccttggttt
     1141 gatcaccgag atgactatca ctgttcgagc acgggctgga tcaggtgata ttgatctgag
     1201 ccaaactgta attgaattga cagatggatc agtaaaaacg ttactcacct atagtcctac
     1261 agcgttacaa ttcaattcga gtattgatga agacggaaat attttcaata ctggattctg
     1321 gaatatgacc aatgaacaat ttggtatcct tgtcttgcag gatgaagatt cctcattaac
     1381 agcgagcaat ccagtcatta accgtgggga tcttgtgatg ttgacaataa atactgatgc
     1441 gtgtttccca gttgcgggtg gactagaccc acgagatgat atttggggtt tggtgtcacc
     1501 tgaacaaggt gcatccggaa tctttgcttt ccgagtacca tcatcactta ccgaacaagt
     1561 ctatgatctc tattgaggag gatgaaaaaa atgagaaata ttaagaaaat gttgaaggaa
     1621 gatgatcttg gtgacatggg tattggtgca atgattgtgt ttattgcgat ggtcctggtc
     1681 gcgggtattg cagcttcagt gttgattcag actgcgaacc gtttagagat tcaagcaatg
     1741 caaacaggtc aagaaacaac tgaagaagtt gcgactggaa ttggtattga agatataact
     1801 ggtcaaattg gagctggtgc gataagtaat atcacaatct gtgtatctcc gcgttcaggt
     1861 tcaaaagata ttgatctttc gaacacggtt gtagagctct cagactcgac aaataaatac
     1921 ctcataatat acaacaccac actcgcatgt aatgttccag cggcaacagg agtatttgat
     1981 acaacagctt tcgctacaga tgcctcattt agtatcattg tcctggaaga cgctgacggt
     2041 tcatgtgtag gtacaaatcc agtgattaat agaggagatc atgttctatt agcaatatcc
     2101 gctgatgcat tatttggtgg actgaccgaa agaactgata tctggggtat ggtcattcct
     2161 gaaagtggtg caccaggtgt ctttgccttt agaacaccag catcatatcc tgatactgtc
     2221 tacgacctct attaaagcgc aaacaaaaac gacaagcttt gggtgtaaaa cacccactta
     2281 tctctttttt ttctttgatt tttctccttc ttaatcagga gtccttaaaa cccatagttt
     2341 tttatgctgt ttagtatttc tgtttatgtg gtgggagtac ttcttcatac cctcggaaac
     2401 acagtgggta tgaagtaaga acgagttcta tgtcgttcca aaaactcaga acaaatctcg
     2461 atgctgacgt tggtatcgga gctttgatta tctttatcgc gatgatcatg gtcgctggag
     2521 tcaccgcttc ggtgatgata cagacgatga ataatctcca aaaccaagcg ataaaaacca
     2581 gtgaagagac gatacgagat atctctagcg gccttcaagt cacctttgtg agcggttatg
     2641 tgcaaaattc aacaatttct caactcgcta tcttcgtcag tactacatca gcgtcggatg
     2701 atgttgatct tacctatgcg attctaactc tttcggattc agcaaagaaa gtaatactca
     2761 attattcaag cacctgtttt cgccaatcca tctccaatgg tttatttgac acgttgaacg
     2821 caagcaacct gacatcatct cagttcggtg ttcttgtgat acgagatatc gatggctcat
     2881 gtaacgcgac aaaacctgtg attgatgatg aggatctggt tgttctcatc gtgaacacta
     2941 ctacatgtct ctcaggaata ggaacacgca ccgatgtgat cggggagatt tatccagaac
     3001 atggtattca tggagtcatt gggtttacca ccccaagtgc ctatgtgcat tccataatcg
     3061 atttacaata attattttat tatttagaat aaaaagttaa acgacttttt tgctcttctg
     3121 ctacatcccg aggtatccgc gtaatccaat gtctaccgct attgaggtga ttgcaacaat
     3181 ccaagcaaag ggaatgaaat aaaaaaaggt gacaatgaaa tggctgcctc gaagcgatcg
     3241 tatcgtcaat cccatgagaa atgaatgcgc aaggaggaca atgaaaataa ggaaaaaatt
     3301 ctgtgtgaag acctcaggag gtacggtggt gagaattgct ccgacatcaa tcgttgttcc
     3361 ttcgaaggga tttcctgcgt tagtgctgag cagaatgcta ttcagatgtc gtgccacgac
     3421 aaatgaaata tagatg
//
LOCUS       DATMXJ010000169         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_34618, whole genome shotgun sequence.
ACCESSION   DATMXJ010000169 DATMXJ010000000
VERSION     DATMXJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34618"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..>4134)
                     /locus_tag="VN377_04400"
     CDS             complement(300..>4134)
                     /locus_tag="VN377_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWR27660.1"
                     /translation="HYMMLSCTPWSDDRISEIPGPYLPIFSVNKTVGEFLVNQGVNSD
                     NTISGYIDQVYYEGTTPSNGIDAYDIVGHINIPKSPEDKIVVISNRQDSWWGECPGDS
                     GAGAGIVLGIAKYFKEHDITPKYNLTFLQTTGEELQMRGAYFYSDNHSEDNIFLFIGT
                     DQLGMEQPGSYLNLNYKSDSTRRIVYAITNETHYVQRTKYQINNTPAFADNAPDNAPG
                     SNWGDSTDDHVWKRRDMYDNSNPCDTIVVNKENWTSHHQTGLNFNEGDSLKNTNRSDL
                     NITFELFWNITKYFCVNPDCEFENLVFTEFDSPADGDTLLDSIRADFSINSVLPNDKV
                     MVNATLCDQNNNGAVVAWKVTNYTITTLSQSHPYNITITIPDTDAQGEYSLQLRLYNS
                     TGRINKIIDSDDDEPNEIQNSDSFHLYHPFGNPTPGNLYHSTEDIIRGSYFTANEYGT
                     ARNITAYVQANTSGTSVHSVCMIYRKNDSKLIGRTVEILPATGRTPQWRIYNFTTPYP
                     VLEEGTEYVLVCWSDGLCNLYYDHIPLTTRGRYDSHTYGVPPDPAVFIPDTSLYSIYC
                     GYRNDTNPPWVTNITENPHTVGFGGNVTISADVVDNESGVNTVTVNIGYPGGRAENKM
                     MTLKSGDTYQYVFNNTWLVGQYNYTIWATDNANNTSNHYDRHHFHVSADASISIATLQ
                     DSYSGNQYINITDPPNIPENYTVVNRGLTWDEYYNAITGQNILEVSTGPVNYQEDNGT
                     WTPINNTISQLAANHPAYVYGYRNGNNHGLYGAYFKSNAQNEWPVAFTYNRSDDPTTY
                     VIRSKLVGVGYVDPQSNWAYQYLQNIQSSQGQFTGNTITYEDVFTGTDVTWSYGNTGL
                     KEEITLSNTTKTLLQNHPPSQYGFNDASSYLVFITKLDHQSLNLYNSSGLLDGNVTIS
                     DNGVEFRDMLGQFKCALPLGEAYESNNESVRQRLTYRIVRYHDETYLLSGLRVTDLNS
                     MTFPVVIDPTITINVSSSSDERGNYNSNYQTAWSATTSNLITDPYIVIGQRKASNIYM
                     IHRGFVFIDTSSLPANAVIDDSVLSLYKYSDSSTTDFSITIQNGQPTYPHDPLQTSDY
                     NKALYSGDGGKLNTASLVNGYNNISLNSTGLGWINRTGWTKLCLRSSRDISGIAPTGN
                     EYVTFYSSTQGSATIPKLIITYRNQSKIKNTGSTDIKGYLLIQVQFYETGKGVAPRWV
                     VDNDTVNETSPRIINSGSQLGLDTVFNGKIRTSNLQHGTGTYRVYTAFRDPEGTILRT
                     NDGTELKAWWQFSKT"
BASE COUNT         1069 a          835 c          926 g         1304 t
ORIGIN      
        1 ataccgttga gatcctcgac gcttttttag gggttatttc ttagtcatta accactagga
       61 tcatatccac agttcccgga gaaaaatcaa gccatgctgt taatagtttt ttgtgtttta
      121 tgatactggt caaattttga aaagatcatt gtgatagtat tatgatcatt ttagattctg
      181 aggaagaagt atctcttggt ggctttgcac ttgttatgat taacaaaaaa aaaaatatag
      241 aaaatcaggc tggattatac tttcctgatc tcttttttta tcactgaaga atactctcgt
      301 tatgttttgc tgaactgcca ccaggctttc aattcggttc catcattagt cctaaggatg
      361 gtgccttcag ggtctcggaa cgcagtgtag acacgatagg tgcctgtccc atgctggaga
      421 tttgatgttc tgattttacc gttgaagact gtgtcaagac ccaattggct gccgctgttg
      481 atgatccggg gggaggtctc attcactgta tcattgtcga cgacccatct cggggcaacg
      541 cctttaccgg tttcatagaa ttgcacctgt atcaacaagt atcctttgat atctgtggaa
      601 ccagtgttct tgatctttga ttggttccgg taagtaataa tcagcttcgg tatcgtggca
      661 cttccttgtg tactcgagta gaatgtgaca tattcattac cagtgggtgc tatgccgctg
      721 atgtctcgac tgctccgcag gcacagtttt gtccacccgg ttcgattgat ccatcccaaa
      781 ccagtactat tgagggagat attgttgtac ccgttgacaa ggctggcggt gttcagctta
      841 ccgccatcac ctgaatacaa ggccttgtta taatcggatg tctgcagggg gtcatgtggg
      901 tacgttggtt ggccgttctg aattgtaatt gagaaatcag tcgtagatga atcactatat
      961 ttatagaggc tcagaaccga gtcatcgatc acagcgtttg caggtaatga tgacgtgtca
     1021 atgaacacaa atccccgatg aatcatatag atatttgatg ccttcctctg gcctatcacg
     1081 atgtacggat cagtaatgag gttgcttgtg gtcgctgacc aggcagtctg gtagtttgaa
     1141 ttataatttc ctcgttcatc actactggag cttacattga ttgttatcgt cggatctatc
     1201 accacgggga atgtcatgct gttcaaatct gttacacgga gtccagagag aagatacgtc
     1261 tcgtcatgat accgaacgat acggtacgta agtctctgtc gaactgattc gttgttcgat
     1321 tcatacgctt ctccaagagg cagagcgcat ttgaactgcc cgagcatatc cctgaattcc
     1381 actccattat ccgagatcgt gacgtttcca tcaagcaacc ctgaactatt atagaggttg
     1441 aggctctgat ggtccagttt ggtgatgaac acaagatagg agcttgcgtc attaaacccg
     1501 tattgtgatg gcggatggtt ctgcaacaat gtttttgtcg tgttgctgag agtgatttct
     1561 tctttgagtc cggtgttgcc gtagctccaa gtgacatcgg tgccggtgaa aacatcctca
     1621 taggttatgg tatttccggt gaattgtcct tggctgcttt gaatgttctg cagatactgg
     1681 tacgcccagt tgctctgcgg atcaacatat ccgacaccaa cgagtttact tctaataacg
     1741 taggtggtgg ggtcgtctga ccggttgtag gtgaatgcaa ctggccattc attctgcgca
     1801 ttggatttga aatacgcgcc atataaccca tgattgtttc cattacgata accatacacg
     1861 taggcaggat gattggccgc aagttggctt atggtattgt tgataggtgt ccaagtgccg
     1921 ttatcctctt ggtagttcac aggtccagtt gaaacctcaa gaatgttttg ccctgtgata
     1981 gcattgtaat attcatccca ggtaagtcct cgattgacta ctgtgtaatt ttcaggtatg
     2041 tttggcggat ctgtgatatt gatatattgg ttgccgctgt agctgtcttg aagagtggcg
     2101 atgctgatag aagcatcagc agagacatgg aaatgatgtc tatcataatg gttacttgtg
     2161 ttattggcgt tatctgtggc ccaaatggtg taattatact gtccgacgag ccaggtgtta
     2221 ttgaatacat actgatacgt gtcacccgat ttaagggtca tcattttatt ttctgctcta
     2281 ccaccaggat aaccgatgtt cacggtgact gtgttcacac cgctttcatt gtccacgaca
     2341 tccgcagaaa tcgtcacgtt ccctccaaac cctaccgtat gaggattctc cgtgatgttt
     2401 gtcacccaag gcgggttcgt atcatttcta tatccgcaat aaattgagta taaactcgta
     2461 tcagggataa atactgcagg atctgggggc acgccatagg tatgagaatc atatcgtccc
     2521 cgtgtcgtga gagggatatg atcgtagtac aggttacaaa gaccgtcact ccaacagacg
     2581 agcacatatt cggtgccttc ctccaagaca ggatatggtg ttgtgaaatt gtatatcctc
     2641 cattgaggag ttcggccggt cgctgggaga atctcaacag ttcttccaat caacttggaa
     2701 tcgttctttc gatagatcat gcacaccgaa tggactgagg taccggatgt atttgcttgc
     2761 acgtatgcgg tgatgttacg ggcagtgcca tattcatttg cggtgaaata cgagccacgg
     2821 atgatatctt cagtactatg gtagaggtta ccgggagtag gatttccaaa cggatgatac
     2881 aaatggaaag aatcagaatt ctggatttca tttggttcat catcatctga atcgatgatt
     2941 ttattaattc gacctgttga attgtagagg cgaagttgaa gtgaatactc gccttgtgca
     3001 tctgtatcag ggattgtgat tgtaatgtta taaggatggg attgacttag tgtcgttatc
     3061 gtataattag taactttcca tgcgacaacc gcaccattat tattctgatc gcatagtgtt
     3121 gcgttcacca tcaccttgtc gtttggtagg acagaattga tggaaaaatc tgctcgtatg
     3181 gaatcaagaa gtgtatctcc atcagctggt gaatcaaatt cagtgaaaac gagattctca
     3241 aactcgcagt ctgggtttac acaaaagtat tttgtgatat tccaaaatag ttcaaaggtg
     3301 atattcaggt cgctacggtt tgtgtttttc agtgaatcgc cttcattgaa atttaaaccg
     3361 gtttgatgat gagatgtcca attttcttta ttaactacga ttgtgtcaca gggattagaa
     3421 ttatcataca tatcccttcg tttccataca tgatcatcag ttgaatcacc ccagtttgat
     3481 ccaggggcat tatctggggc attatcggcg aaagcaggag tattattaat ttgatacttc
     3541 gttcgttgaa cataatgagt ttcattagta attgcatata caatccttcg agtgctatcg
     3601 ctcttataat ttaaatttaa gtatgatcct ggctgttcca tacctaattg atctgtaccg
     3661 ataaatagaa agatattatc ttcgctatga ttatcactat agaaatatgc tccacgcatt
     3721 tgtagctctt ctccggtggt ctgaaggaat gtcagattat atttcggtgt gatatcatgt
     3781 tctttgaaat atttcgcgat accaagaaca atacctgccc cagcgccaga gtcacccggg
     3841 cattcacccc accaagaatc ctgtctattt gaaatgacaa caattttatc ttcagggctc
     3901 ttcgggatat taatatgacc aacgatgtca tatgcatcta ttccattact tggggtcgtt
     3961 ccctcataat acacctgatc aatataacca gatatggtat tatcggaatt cacaccttga
     4021 tttactaaga attctcctac ggtcttgtta actgaaaaaa ttgggagata tgggccaggt
     4081 atttcagata tacgatcatc actccagggt gtacaactta acatcatata atgt
//
LOCUS       DATMXJ010000170         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_34685, whole genome shotgun sequence.
ACCESSION   DATMXJ010000170 DATMXJ010000000
VERSION     DATMXJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34685"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(465..1040)
                     /locus_tag="VN377_04405"
     CDS             complement(465..1040)
                     /locus_tag="VN377_04405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWR27661.1"
                     /translation="MRSIQEIESIIDSLPSPRVTPKITQTKRKKSFWKTLTVIGISLC
                     TILVAMMVLTGMLQVLIVLSGSMQPTFNAGDVILTVDTPVRSLKVNDIITYWSPDNMK
                     SLVTHRIVTISKDSQGLSFQTKGDANEGVDQYVVPAELVVGRMVLTIPYVGHVASISH
                     SFLGFLLLVLAPGIVVISAEVLSIVKKEKTT"
     gene            complement(1037..2044)
                     /locus_tag="VN377_04410"
     CDS             complement(1037..2044)
                     /locus_tag="VN377_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27662.1"
                     /translation="MCIEKGFGAINPWEWPVEHLTIGGVLYTKAEAIRVMHTHCAQDK
                     TYDLFRELAATMLNLFCGCNPECIQETVECADHWLVAHPVGSDVHANSHAWKLAKQWW
                     KQLEQYNKGLLCEESCNCPPDSPRDCSQHSKHPVTMHQLQVYVSDVDYDELSVSFYNA
                     CNDSIMGEYSMVESDSIVSLSLDEVSANESFSWYVIVSDQQAATMSPTWTFTIDANDT
                     ETMNGDNETSDNTSSPPGSFDFVDLSPLTADAHTTYTFQVQALNNSSVPEIMVVYWCN
                     ETTQTSLMLCDHEGSWNASIIIASNVTRFWYRLYIAYETGLWNPMEEHEVSIPDGGDG
                     P"
BASE COUNT          715 a          592 c          482 g          687 t
ORIGIN      
        1 atattatcag gataaccatc attatctgaa tctgcagtat tggagtttgt attggaatta
       61 ttatcttgtt gagtacatcc attcaataaa catacagtta ttatcgttgt aacaaacaat
      121 actagtgtta tttttttcat aacctttgtc tcaagcttgc agatttgaag caatttctcc
      181 cttaaaaaca ttcctttagc ctcattttat acgatattaa acgaagcctt ttgtaataat
      241 ctgattttcc ctttcaattc ttttgaatca attaaagaaa tcaacattgt tattcttgtt
      301 ctatgagctg agtcagccat tttctttctt agatctgcac tgaactttat tatttcagtt
      361 ttcttaaata aaaaaaataa aaccagattc ttttattaag tgataaatta atcttatttt
      421 tttaaaaaaa atcgagggaa agcaaggtta taggtacgga actcctatgt tgttttttct
      481 ttcttaacga tacttaggac ttcagcacta atcaccacaa taccaggtgc gagaaccaac
      541 agcagaaacc cgagaaacga atgagaaatc gaagcgacat gcccgacata cggtatcgtg
      601 agcaccatgc gaccgacaac cagctcagca ggcaccacat actgatcaac gccttcattc
      661 gcatcacctt tcgtctgaaa cgaaagaccc tgactatcct tacttatagt aacaatgcga
      721 tgcgtcacca gactcttcat gttatccggt gaccaatacg tgatgatatc gttgaccttg
      781 agcgaacgaa ccggggtatc aaccgtaaga ataacatcac cagcattgaa cgtcggttgc
      841 atcgagccgc tcaacacgat caacacctgc agcatccctg ttaacaccat catcgcaaca
      901 aggattgtac acagggaaat gcctatcacc gtcagtgttt tccagaatga ttttttcctc
      961 tttgtttgtg ttatttttgg agtgacgcgt ggtgagggaa gcgagtcaat aatagattcg
     1021 atttcctgaa tgctcctcat ggtccatcac ctccatcagg tatcgatact tcatgttcct
     1081 ccataggatt ccacagtccg gtctcatacg caatatacaa ccgataccag aaccgtgtca
     1141 cattcgacgc aatgatgatc gatgcgttcc agctcccttc atgatcacag agcatgagac
     1201 tggtctgtgt cgtctcgtta caccagtaca ccaccatgat ctccgggaca gaactattgt
     1261 ttaatgcttg aacctggaat gtatacgtcg tatgagcatc cgctgtcaaa ggagacagat
     1321 cgacgaaatc gaaagatcct ggtggcgatg atgtgttatc agatgtctca ttatcaccat
     1381 tcatcgtttc cgtatcatta gcatcaatcg tgaacgtcca tgttggtgac atggtcgccg
     1441 cttgttggtc agagacaatc acataccagc tgaacgattc attcgcagag acttcatcca
     1501 gagaaagtga cacgatgctg tcgctttcca ccatagaata ttcccccatg atgctgtcat
     1561 tgcaggcgtt gtagaaactg accgaaagct catcataatc aacatcagag acatacacct
     1621 gcaattgatg cattgttaca ggatgcttag aatgctggga acaatcacgc ggagaatccg
     1681 ggggacagtt acaggactct tcacagagca accccttgtt gtattgctca agctgcttcc
     1741 accactgttt cgcaagcttc catgcatgag aattcgcatg aacatcgctg ccaaccggat
     1801 gagcgaccaa ccaatggtca gcacactcaa ccgtctcctg gatacactca gggttacagc
     1861 cgcagaaaag attcagcatc gttgcagcaa gctcgcggaa caagtcatag gtcttatcct
     1921 gcgcacaatg cgtatgcatc acccggatcg cctctgcttt tgtgtacaag accccaccga
     1981 tggtcaagtg ttccacaggc cactcccaag gattgatggc accaaaaccc ttctcgatgc
     2041 acacaaggtg tagatcccat acggaacgtc gccgtgatgg tctccgtatc agaaaacgat
     2101 gaagtcgtac cagaaaagga aggtatgagc cttggcagcg agaacacaca gacgagaagg
     2161 aataatacat gaatcaatct gaattgtttt ttcccttgtt ttcctaacat aaccattttt
     2221 ttccctatta tttctcttgt gaacgttttt tttccaggcg aaaaagaaag aaagagagga
     2281 ggaaagagaa tactctcccc cgtcagcgtc aacgctgtac ttttatgatt ttatggtacc
     2341 tgattcatgg tcgcttcgat ggtaacgtct atcccgtcga actgatactc attaccgaca
     2401 cccgggtcaa gcttcacctg aagggtctca taatggacat cgccaggagc aaataccgca
     2461 tcagttccat gatacg
//
LOCUS       DATMXJ010000171         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_55816, whole genome shotgun sequence.
ACCESSION   DATMXJ010000171 DATMXJ010000000
VERSION     DATMXJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55816"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="dnaG"
                     /locus_tag="VN377_04415"
     CDS             <1..1161
                     /gene="dnaG"
                     /locus_tag="VN377_04415"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HWR27663.1"
                     /translation="ELDLRELQRSARVGRIEVELDSKNGKSDGVLTLPTSLDKVETVI
                     LAAAFESIDRVGPCKATIHVEEVEDVRVSRRKQVIERAKVLLNEVMERSKTDSESISE
                     NVRQAVQVEEITSYGHDHCPAGPNINDSDAIVIVEGRRDVLNLLKYGIKNVIAVGGTN
                     VPRTVIDLSKEKITTVFVDGDRGGELILRELLQVADVDFIARAPQTREVEEIPQKLIM
                     KSLKNKIPREQYMDMYNIKIEGKNGEDKRLDNGEKQAPKMKKEFFSKKKEQQQTRKEE
                     DSAPEVVEEPVISTEKQKNYEAILNEISGSLKAVLLDENGKEIRTIPVRELTDEIKKG
                     DNVSAIVFDGIVTQRLLDIANNKNVREIVGIKLGNVVKMPKAVKVLTKNDFQ"
     gene            1281..1583
                     /locus_tag="VN377_04420"
     CDS             1281..1583
                     /locus_tag="VN377_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HWR27664.1"
                     /translation="MQKDELIQLHTFLYQIRSQLEQICEHNGCDHSAYEKLEITPYQI
                     HKSKREHKLAVFTLSKDIAGILSDNNGDPGLEKIANRLDQMAIRFMTEKEKVTHRS"
     gene            complement(1578..2015)
                     /gene="rimI"
                     /locus_tag="VN377_04425"
     CDS             complement(1578..2015)
                     /gene="rimI"
                     /locus_tag="VN377_04425"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWR27665.1"
                     /translation="MLSIRRVQPNDIFPVIALAFETLPERYNPSIFTQFYESFPEGFL
                     VALNNETIIGFLIGIRTTPNVARILMLAVKDTYRKQGIGSSLLAQFIKEMRQQYVTSV
                     ELEVRTSNKGALRFYQARGFLLQDTLQQFYQNGENAYCMRKEL"
     gene            2103..2876
                     /locus_tag="VN377_04430"
     CDS             2103..2876
                     /locus_tag="VN377_04430"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HWR27666.1"
                     /translation="MSIDDVTVTRIIFERFSKEFSDSFDVDVAIAGAGPAGLTAARYL
                     ANAGKKTILFERRLSVGGGMWGGGMTFPVIVVQKESKHILEDVGVRIKDEGKGYYSAD
                     SIEVTTKLCSAAIDAGVRIFNTISVEDVLMDKKKVNGFVINWSAVEIAGLHVDPLSIR
                     ARYCIDATGHAAEICHIVRRKVGALNTPSGDLEKERSMCAEIGERTVVENTKEVYPGL
                     FIAGMGANAVYGAPRMGPIFGGMLLSGEKVAKLILKKLK"
     gene            3050..>3392
                     /locus_tag="VN377_04435"
     CDS             3050..>3392
                     /locus_tag="VN377_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27667.1"
                     /translation="MYKKNFRKNERGDVLGIPMYLIVIMIVAVAVIAAVVFMIPKATR
                     AMSVQVTSSALIAESPGSDGGGKFTFSKTYTIWVKVTTRDERADPISEATVTLVGAGD
                     AGQGKTLDNGSA"
BASE COUNT         1012 a          683 c          885 g          812 t
ORIGIN      
        1 gaactagacc tgcgagaatt acaacgatcc gctcgggtcg gacgaatcga ggtagaactt
       61 gattccaaga acggcaaaag cgatggtgtt cttacgctcc caacatcctt agacaaagtt
      121 gaaaccgtta tcctcgctgc agcgtttgaa agcatcgatc gggtcggacc ctgtaaagcg
      181 acgatccatg ttgaggaagt cgaggatgtt cgtgtctcgc gaagaaaaca ggtcatcgaa
      241 cgagcgaagg tattactgaa tgaggtcatg gagagaagca agaccgacag tgagagcatt
      301 tcagagaacg tccgacaggc ggtccaggtc gaggagatca ccagttacgg tcatgaccat
      361 tgtcctgctg gcccgaatat caatgatagc gatgctatcg tgattgttga aggcagacgc
      421 gatgtgctca acttgttaaa atatggtatt aagaacgtca tcgcggttgg tggaaccaat
      481 gtcccacgga ccgtcattga tctttcgaaa gaaaaaatca ccactgtttt cgtcgatgga
      541 gatcgcggtg gggaactcat cctccgagag cttttgcaag ttgctgacgt tgattttatc
      601 gcacgggcgc cacagacacg agaggtggaa gaaatccctc agaaactcat catgaagtca
      661 ttgaagaaca agatcccccg tgagcaatac atggatatgt acaacatcaa aatcgagggg
      721 aagaacggtg aggacaagcg tttggataat ggcgagaagc aagcgccgaa gatgaaaaaa
      781 gaattcttct caaagaaaaa ggaacaacaa cagacccgca aggaagaaga tagtgcccct
      841 gaggttgtcg aagagccggt gatttcgaca gagaagcaga agaattacga agcgatactc
      901 aatgagatct ccgggtcatt aaaagctgtg ttgttagacg agaacggcaa agagatacgg
      961 acgatacctg tccgggagct cactgacgaa ataaagaaag gtgataatgt ctctgccatt
     1021 gtctttgatg gaatcgtgac gcaacgcttg ctggacattg cgaacaataa gaacgttagg
     1081 gaaatcgttg gtattaagct gggcaatgtg gtcaaaatgc ctaaagcggt caaagtgttg
     1141 acaaaaaacg attttcaata aatttataac atacaactga cgttaacaat catgtcaatg
     1201 tgaagcaggt acagacacgg ctgcctagaa aaaatataaa agtcgttata ctattcttgg
     1261 caagggaaag gacaaaaaca atgcagaaag atgaactcat acaactccac acgtttttat
     1321 atcaaatccg aagtcaattg gaacagatct gtgaacataa cggatgtgac cacagcgcct
     1381 atgagaaact cgaaatcaca ccgtatcaga ttcataagtc gaaacgtgag cataaactag
     1441 cagttttcac cctcagtaaa gatatcgcag ggatcctctc tgacaacaac ggcgaccccg
     1501 gcctggagaa aatagcgaac cgtcttgatc agatggccat ccgcttcatg accgagaaag
     1561 aaaaagtgac acatcggtca taattcttta cgcatgcaat acgcgttttc cccgttttga
     1621 tagaactgct gtaacgtgtc ttgaagcaga aagcctctag cttgataaaa ccgtagagca
     1681 cctttgttgg acgtgcggac ttcaagctcc acgctggtga catattgctg tctcatctct
     1741 ttgatgaatt gtgccagcag tgatgaaccg attccttgtt tgcgatacgt atcttttact
     1801 gcaagcatga ggatccgtgc gacgttcggg gttgttctta taccgatgag aaacccgata
     1861 atcgtctcat tgtttagagc cacgaggaaa ccctcaggga aagactcgta gaactgcgtg
     1921 aagatagagg ggttatagcg ttctggtaat gtctcaaacg caagagctat gactgggaaa
     1981 atatcgtttg gctgcacacg tctgatactc aacacgagca ggaaacaccc cctgggctat
     2041 aaatcattag cggtatgttt aagaatctcc gaaaaatatg ggtcgatgtc gaggtgttta
     2101 gtatcagtat cgatgatgtc acagtcacac gtattatctt tgaacgattt tcgaaagagt
     2161 tcagtgatag ttttgatgtg gatgttgcta ttgctggggc tgggcctgcg ggcttgactg
     2221 ctgcaagata cttggcaaac gcagggaaaa aaaccatcct attcgagcgg agactctctg
     2281 ttggtggcgg catgtggggt ggggggatga ccttcccggt tatcgtcgta caaaaagaat
     2341 cgaagcacat cctggaagat gttggtgtgc ggatcaaaga cgagggaaaa gggtactaca
     2401 gtgctgattc tatcgaggtg accacgaaac tgtgttcagc cgcgattgat gccggtgtcc
     2461 ggatttttaa tacgatttct gttgaggacg tgctgatgga taaaaagaag gtcaatgggt
     2521 ttgtgattaa ctggagtgca gtcgagatcg ctgggttaca tgtggacccc ttgtcgattc
     2581 gagcacgcta ctgtattgac gctaccggtc atgctgctga gatctgtcat atcgtacgcc
     2641 ggaaggtcgg agcgttgaac acgccaagtg gtgatcttga gaaagaacga tccatgtgtg
     2701 cagagatagg ggaacggacc gtcgtggaaa acacaaaaga ggtctatcct ggtctcttta
     2761 tcgctggcat gggtgccaat gcagtctatg gtgccccacg gatgggcccg attttcggtg
     2821 gcatgcttct ctcaggagaa aaagtagcga aattgatcct taagaaactc aaataacgct
     2881 attcatctga ataagatgag gcattttttt cttttcggaa ccaagcaaga aagcgatgat
     2941 caaaatgtgt catcgttgta ttataaaaat tttattgcaa aagaattaaa aagatacatg
     3001 tatatatctg ctttcatcaa atatcttaaa agtcgggagg taaaaagaaa tgtataaaaa
     3061 gaattttagg aagaacgaac gtggggatgt gttaggcatc ccgatgtacc tgatcgtaat
     3121 catgattgtt gcagtcgcag tgattgcagc ggtcgtcttt atgatcccga aggcaaccag
     3181 agcgatgagc gtacaggtga cctcgagcgc acttattgca gaaagcccag ggtctgatgg
     3241 aggcggaaaa ttcacgtttt cgaagaccta cacgatttgg gtgaaagtca ccacccgtga
     3301 tgaacgcgca gacccgatta gcgaagcgac cgtgacattg gtcggggcgg gggatgctgg
     3361 tcaagggaag acccttgata atggttctgc ta
//
LOCUS       DATMXJ010000172         8864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_8401, whole genome shotgun sequence.
ACCESSION   DATMXJ010000172 DATMXJ010000000
VERSION     DATMXJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8401"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..5856
                     /locus_tag="VN377_04440"
     CDS             211..5856
                     /locus_tag="VN377_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27668.1"
                     /translation="MHISDGLRAYGIDRKAVIVVMVLPFLLVSLNCVEASPEKDLAFP
                     SHVSLGDSVLIAGAFQYLDVTLPTEHEKICIIAFNGDTEPKPQDRSENNYYRWEYDNG
                     AWNDTSGYDISYIDSSQCSKQNTTYLFYLRTSQKVNPGSWTIKVLVDDIEASSISFQV
                     VIGDFCLFFSTIIGVFEPTMKQKNLLSEDELRCCYQQKKQEVSEANIERFVDSVLKKH
                     TIESNKDDSKRKTRDLYHPSDDFESTQEPIRTAISLYPRTKLKEITNEKTNTLFFWRT
                     QTGGDSFCSYRSEGLKRFFIILVLTLLLTASIVPMVVLMGRNENTCDITIINNHSYPL
                     IGGNWTVLFTMVGCADLTITAVNRTSWGNSDPDADLKFLHVDNGNKTVDCYWMNDSVY
                     IPNFSSNVTCSEISQVLTPGVHVLMFQFGDDIAFAYNLASENWLQTSTSDFNNGTKTN
                     INVNSGSFHLQERYYLRNFTRINNEGFEGSWPPVDWSEVPTTSNWNAENYEVYEGSYS
                     ADFDGAGGAGGASGNLLSPSMNCAGSNVTAIYVKFWGYSDAADNGEYFLDYYDGSTWD
                     QITRLDDFGQDVWAQYSQKVTDSQYFKSNFQIRWRVVGLNNNEHVYVDLVNVTVERNE
                     SGYHSAGSLLSAVHDTARNMPDYNNIHVGNTAPSGTTITTWVRAAGTQANLSTATWYS
                     TINQVPDKRWVQWRINLTGNTYLTPTINDVNLTWTYDNEYPISSVNTLMPYWQKTTPF
                     QISVTASDNGTGIKEVALYCNYSANNVSGWSGWSRYGTNDTTSPYTWSFTPPQGDGYY
                     RFYSRAIDGELNNEAAPSTPGFDASCGVDTVKPSSHLSEIIPYWYIEPDRDVIINCSS
                     ASDSLSGLQNILLYYRYRVDNGSSWGSWQLFSSDTAAPWYWIFNFPKAKGFYQFYSIA
                     VDNAGNAENPPTAPDNDTDCAYNSTKPYSEVNAISPYWHSTSLTITAQATDFNGSGLL
                     NVTLYYYYSSNNNTWSTSYLFGVDTDPWNANSWVFTFPNGSGYYRFYSLAIDNDTNVE
                     YFTGNDTLGGYDVVQPSSQVYQISTYWHSTSENPLLITVTNTSDSLSGVKNVTLSYRY
                     RADNTSSWNAAVPFSSDENAPWSWNFTFPNGNGHYRFYSLATDHAGTREDPPTSPEYD
                     TQCGYDTSKPSSQVAVISPYEVTVSPFLISATASDDTKNVTIWYYYSPQNSSWWNPNW
                     HYRKQLNISAKNGGYQMRIIIGNTSGGNVTCNGHTRSNFGDIRFVSYSDNSTALSYWL
                     KNYTAGAQATFWVNNSRNDTSIWLYYGNSNALTTSSGDSTFYFFDDFSNGLSKWVMDS
                     WNTDSILINQSQGNPSPALKHLPDNSIPANRTYQDTRIRTAYKILNGIIEYDVYLAGT
                     PRIIHQFGWRVNSLSWTNGYCWRVQNSAADGGFFEFSAPSTWTQFGAAFPLISTSTWY
                     HVKINVSSATYSAKITPSAPAGDSARSATDSTKTTADYLISHVHGVSMNSGNYALVDN
                     VFVRKYWATPPTWGSAGAEQSGYGEWNNASNPDPSPSWGWNFDFPGSFGYYWFYSVAV
                     DVNGDKEDPPNSADARCRYEMPIAPVINSYDLRNESGSKLNNATGLLDVNSEYYFTIN
                     VTAKFGWFYIDYIDIIAWYDQGSETSLYNQTTGGNLNMYLRYENVTGNASFRILWPKY
                     EVQLVTSNCTQTIVNNSTRIIKISFKPLNQTRWACSNNTWDTTKNTTNDPFSWNFNIT
                     AIDTSGLKAWKNDEYGIYKFALLLPDKNWVDVRAPPGYNATTNIVNITYCSNYNYNIS
                     IYFEENLTNVSSGDIIPIANNVYICANADMDDDITTDMMFNGIQATNAIDIINTSGIF
                     HKNNTSQYVHVQFNVFIPFGTIQGEYTAHVATKIKFKER"
     gene            6011..7117
                     /locus_tag="VN377_04445"
     CDS             6011..7117
                     /locus_tag="VN377_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27669.1"
                     /translation="MGKQNIYTVLVVAALVISLFTGMFCLEPFIIKIAKANPGDINEQ
                     WYNETTLNVTILYREPRFNWYDFQYNQSGTWVSRLNMQSDVNDSAEYRFIVNISADNG
                     WENITYINITAWYDQGDESTTYNQTLGGNQNLFFQYENLTGTANWRMLWPTGGEVTGS
                     QFSERVVNDPVGSPRFTECHNITFSFVPGYQFRYAPGDGSWNTSDNATNDLESWNFKI
                     YASNEQGYVSWIQNEFGIYSYTEIISAGWPEIFGYPGENATAETNITLLTRSNGNYSL
                     SVDVGNLTHNTHPTANMSRKLVWLRGGDLAISSNFTTFTDLLYLYGSAGTYHLAQANG
                     TSLTTNDVEYKCNIPLGQTAGEYTAPISYHLMTT"
     gene            7343..8494
                     /locus_tag="VN377_04450"
     CDS             7343..8494
                     /locus_tag="VN377_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27670.1"
                     /translation="MEWKKVKKMEMEQKRKLFSTLVGITIITSLVINIFLIGPIIMIG
                     KADPGDIPHIWHNSTTLNVTVLHLEPRILWYDYQYNQSGTWVSKLNAQSDVNNSAEYR
                     FIVNISSDQGWDDIEFVDITAWYDQGNDSSIYNQTRGGNWNLNLQYENTTGTAVWRML
                     WPHNGEFIQGTYTDRVGKDPYGSTGFTECHNLSFSFIPSYQVRYAPGDGAWDTTYNTT
                     TDNESWNFIITITDSGEDAPNPITIWTADEFGIYSYSEIVSAGWPTIIGHPGENATAN
                     SNISLITRSNGNYSLTTDVTTLVHRTFPGATISRERIWVRGGDLDIFNNFTATPGGIY
                     FYGSLSAYHLAQANGTALTTDDVQYKCDIPLGQIAGDYVAPIRYHLTTT"
BASE COUNT         2653 a         1785 c         1938 g         2488 t
ORIGIN      
        1 cacaatgcgt acgttattga tgaaaagcag cagcgattgc caaccgtagc atgaccgccg
       61 agtgagatac ggcaacgccc gatagggctt gccgaggaac gaaggcggaa cggagttata
      121 ggggtggaac ccctatttca tcctgattca ccgaaaagaa aacaaacgtg aaaggaagat
      181 ataaataagg aaaagtgata cttttttcgg atgcatattt cggatgggtt gcgagcctat
      241 ggcatagaca gaaaagcagt cattgtcgta atggtactgc cgttccttct ggttagtctg
      301 aattgcgttg aggcgtcccc agagaaagac cttgcattcc cgtctcatgt gtcactagga
      361 gattctgttc ttatcgcagg cgcgttccaa tatctcgatg tgacgttacc aacagaacat
      421 gaaaaaatct gcataatcgc attcaatgga gataccgaac ccaaaccaca ggatcgttct
      481 gagaataatt attacagatg ggaatacgac aatggtgctt ggaatgatac cagtggatac
      541 gacatttcct acatcgattc ttctcaatgc tcgaagcaaa acactaccta tttgttttat
      601 ctgaggacct ctcaaaaggt gaatcctggt agctggacga ttaaggtact cgtcgatgac
      661 atagaagcct catcaatatc gtttcaggtg gtcatcggtg atttttgtct gtttttttcc
      721 acgattattg gcgtttttga acctacaatg aaacaaaaga atctgctttc tgaagacgaa
      781 ttacgatgtt gttatcagca aaaaaagcag gaagtatctg aagcaaacat tgaaagattc
      841 gtcgatagcg tccttaaaaa acacacaatc gagtctaata aagatgactc gaagcgaaaa
      901 acgagagatt tgtaccaccc ctcagatgat tttgaaagta cccaggagcc gatacgaaca
      961 gcgatttctc tgtatcctcg aaccaaacta aaggaaataa ctaatgagaa aaccaacaca
     1021 ttgttttttt ggagaacaca gacgggaggc gacagttttt gttcatacag atcggaaggt
     1081 ctgaagagat ttttcatcat tcttgtactg actcttttgt taaccgcttc cattgtaccg
     1141 atggttgtgc tgatggggag aaatgaaaat acgtgtgata tcacgattat caacaatcac
     1201 agttatccgc ttattggtgg taactggaca gtgcttttta caatggttgg ttgcgcggat
     1261 ttgactatta ccgcagtcaa cagaacaagc tggggtaatt ctgatccgga tgctgattta
     1321 aaatttttac acgttgacaa cggaaacaag actgtagatt gttattggat gaatgattcc
     1381 gtgtatatcc caaatttttc ctccaacgtt acatgttctg aaatctctca agtccttact
     1441 ccaggggttc acgttcttat gttccaattc ggtgatgaca tcgcctttgc ctataatctc
     1501 gcctctgaga actggctgca aacaagcaca agtgatttta ataatgggac gaaaaccaac
     1561 attaacgtca acagtggttc tttccatctg caagaacgat attatcttcg taacttcacg
     1621 cgtattaata atgagggatt tgaggggagc tggccacctg ttgattggtc tgaggttcca
     1681 acaacaagca attggaatgc ggaaaactat gaggtatatg aggggagcta ctcagcggat
     1741 tttgatggag ctggtggagc gggtggcgcc tcaggtaacc tcctttctcc atcgatgaat
     1801 tgtgctggtt ctaatgttac tgcgatctat gtgaaatttt gggggtattc ggatgctgct
     1861 gataatgggg aatacttttt agattactac gatgggagta cttgggatca aattactaga
     1921 ttagatgatt ttggacaaga tgtctgggct cagtattcac agaaggtcac agattcgcag
     1981 tacttcaaat cgaattttca gatacgatgg agggttgtgg ggctcaacaa caatgagcat
     2041 gtctatgtcg acctggtcaa tgtgacagtg gaacgaaacg aaagcggtta tcattctgca
     2101 ggtagtttgc tttcagcggt acatgatacc gccaggaata tgccggatta taacaatata
     2161 catgttggaa atacagcgcc gtctggtact acgatcacaa cctgggtacg ggctgctggt
     2221 acgcaggcga atctttccac agcaacctgg tattcgacca tcaatcaggt tcctgataag
     2281 cggtgggtgc agtggaggat aaacctcacc ggtaatacgt atctgactcc aacaatcaat
     2341 gatgtgaatc tcacttggac atatgataat gagtatccga tctcatctgt caatacgttg
     2401 atgccctatt ggcaaaagac aacgccgttt cagatatcgg tcacagcttc ggacaatggg
     2461 accgggataa aagaggttgc tttgtattgt aactacagtg cgaataatgt atcaggctgg
     2521 tctggttgga gcagatacgg gacgaacgac acaacgagtc cctacacctg gtcgtttacc
     2581 ccaccacaag gtgatggata ttatcgtttt tacagtaggg caatcgacgg agaattgaat
     2641 aatgaagctg ctccaagtac tccaggtttc gatgcgtctt gtggagtcga tacggtgaaa
     2701 ccttcatcac atttaagcga aatcattccc tattggtata tcgaacctga ccgagatgtg
     2761 attataaatt gctcaagcgc tagtgattct cttagcggat tacaaaatat tttgttgtac
     2821 taccgatata gagtcgataa tggatctagc tgggggtcat ggcaactttt tagttcagat
     2881 acagccgccc cgtggtattg gattttcaac tttccaaaag ccaaaggttt ctatcagttc
     2941 tacagcatcg cggtcgataa tgctggtaat gctgaaaatc ctccaactgc accagataac
     3001 gataccgatt gtgcctataa ctccacgaag ccatattcgg aggtaaacgc gattagccca
     3061 tattggcata gtacatcgct aaccattact gcgcaggcga cagattttaa cggcagcggt
     3121 ttattgaatg tgacgctata ctattactat agttcgaata ataatacctg gtccacttcc
     3181 tatttgtttg gtgtggatac tgatccctgg aacgcgaatt cttgggtttt tactttccca
     3241 aatgggagcg gctattatcg attctacagt cttgcgatag ataatgatac caatgtagaa
     3301 tattttactg gaaatgatac gttgggtgga tatgatgtcg tacaaccctc ctcacaggtc
     3361 tatcaaattt ccacctattg gcatagcacc tcagaaaacc ctcttttaat taccgtaacg
     3421 aacacgagtg atagcttatc tggagtgaaa aatgtcacgc tttcctatcg gtatcgagca
     3481 gataatacat caagttggaa tgctgcagtg ccatttagta gtgatgaaaa tgccccctgg
     3541 agttggaatt ttacgtttcc caatggtaat ggccattatc gtttctacag tctcgccacc
     3601 gaccatgcag ggacaagaga agatccgcca acatcacctg agtacgatac acaatgtggc
     3661 tacgatacat ccaaaccatc ctcccaagtt gctgtcattt cgccttatga agtcacggta
     3721 tcaccgtttc tcatcagcgc aacggcgagt gatgatacga aaaacgtgac gatatggtat
     3781 tattattccc ctcaaaattc gagctggtgg aatcccaact ggcactatag aaaacagctc
     3841 aacatatcag cgaagaacgg aggatatcaa atgcgaatca ttatcggtaa cactagtggt
     3901 ggaaacgtta cctgcaatgg tcatacaaga agcaattttg gtgacatccg cttcgtcagc
     3961 tattcagata actccacagc actatcttat tggctcaaga actacaccgc cggagcacaa
     4021 gcgacatttt gggtcaataa ttcgcgcaac gatacttcga tttggttgta ttatggcaat
     4081 agtaatgcct taacgacaag cagcggagat agcacgtttt atttcttcga tgatttttca
     4141 aatggattga gtaaatgggt gatggactca tggaatactg attcaattct tattaatcaa
     4201 agccaaggaa atccaagtcc cgcgctgaaa catcttcccg ataattcaat tcctgccaac
     4261 agaacctatc aggacacaag gattcgtacc gcttataaga tactcaacgg gattatcgaa
     4321 tacgatgtgt acctggctgg gaccccaagg attatccatc agttcggatg gagggtcaat
     4381 agcttatcct ggacgaatgg atattgttgg cgggtgcaga attcagcggc cgacggaggt
     4441 ttttttgagt tttccgctcc ttcaacctgg actcaattcg gagcagcatt tccactcatt
     4501 tctacaagca cttggtatca tgtcaaaatt aatgtttcga gtgctacgta ttcagcaaaa
     4561 atcacaccct cggctcccgc tggtgatagc gcccgttcag cgactgattc tacaaagaca
     4621 actgctgatt atcttatctc tcatgtgcat ggagtaagta tgaattcggg aaattatgca
     4681 ctggtggaca atgtatttgt caggaaatat tgggcaacac caccaacatg gggtagtgct
     4741 ggggctgaac aatctgggta tggagaatgg aacaatgcaa gcaatccaga ccctagtcct
     4801 tcttggggtt ggaatttcga ttttccaggt agctttggct actattggtt ttattcggtt
     4861 gctgttgatg taaacggcga taaggaggat cctccaaact cggcggatgc tcgatgtcgg
     4921 tatgaaatgc cgattgctcc agtgattaat agctatgatt tgcgaaatga gagtgggtct
     4981 aaactcaata atgcgactgg cttgcttgat gtgaatagcg agtactattt caccattaat
     5041 gtcaccgcga aattcggctg gttctatatc gattatatcg acattatagc atggtatgac
     5101 caaggaagtg aaaccagctt gtacaatcaa acaacgggcg ggaacctcaa catgtatctc
     5161 cggtatgaaa atgtgactgg gaatgccagt tttaggatac tatggccgaa atatgaagtg
     5221 caacttgtca ccagtaattg cacccaaacc attgtcaaca actctacaag aattatcaaa
     5281 ataagtttca agcctctcaa tcagacccgg tgggcttgta gcaataacac ttgggataca
     5341 accaaaaata cgacaaacga tccgttctct tggaatttta atatcactgc gattgatacc
     5401 agtggtttga aagcatggaa gaatgatgaa tatgggattt acaaatttgc tctgttacta
     5461 cctgataaaa actgggttga tgtcagggct ccacccgggt ataatgcaac aaccaatata
     5521 gtgaatatca cgtattgttc aaactataat tataatatca gcatatattt tgaagaaaat
     5581 ctgacgaatg tgtcatcagg ggatattatc ccaattgcaa ataatgtgta tatctgtgcg
     5641 aatgcagata tggatgatga tatcacgact gatatgatgt ttaacggaat ccaagcgaca
     5701 aacgcaattg atatcatcaa cacatccggt atttttcata agaacaatac cagtcaatat
     5761 gttcatgtcc aattcaatgt tttcatcccg tttggtacta ttcaaggaga atatacggct
     5821 catgttgcaa ctaaaataaa atttaaagaa cgttagatga gacgaaatat attccttgtg
     5881 tgtttctaga aaatcatttc agaagatttt gacccaaagt tttatatgat agacaatgaa
     5941 ttagcaactt gcaccctaac atattatcat acaccattgc atcagataaa taaatgtgag
     6001 gactagaagg atgggaaagc agaacatata tacagttctc gttgtagcag cgttagtaat
     6061 atcattattt actggcatgt tctgtcttga accttttatc attaaaattg cgaaagccaa
     6121 tcctggagat atcaatgaac agtggtataa cgagacgacc ttaaatgtca caattctata
     6181 cagagagcca cggtttaatt ggtacgattt tcagtacaat cagtctggca cttgggtctc
     6241 tagactcaac atgcagagtg atgtgaacga tagtgcggaa tataggttta ttgtcaatat
     6301 cagtgcagat aatgggtggg aaaacatcac gtatatcaat atcactgctt ggtatgacca
     6361 gggtgatgag agcactacct ataatcagac gttaggggga aaccagaatt tattcttcca
     6421 gtatgaaaac ctaaccggga ctgctaattg gcgcatgtta tggccaactg gtggggaggt
     6481 taccgggagt cagttttcag agcgagttgt gaatgatcct gtaggtagtc caagatttac
     6541 cgaatgtcat aatataacat tttcattcgt gcccgggtat caattccgat atgctcctgg
     6601 tgacgggagt tggaatacgt cagataatgc aacgaatgat cttgagtctt ggaattttaa
     6661 aatctatgct tcaaacgaac aaggatatgt ttcttggatt cagaatgaat ttgggattta
     6721 ctcctatact gagataatca gcgcaggatg gcctgaaatc tttgggtatc ctggggaaaa
     6781 tgcgaccgct gagacaaata ttactttgtt gacacgttca aacgggaatt attctctctc
     6841 cgtggatgta ggtaatttga cgcataatac tcatcctact gcgaatatgt ctcgtaaatt
     6901 agtatggttg cgaggtggcg atctagctat ttcgagtaat tttactacct tcactgatct
     6961 gttatatcta tatggatctg caggaactta tcatcttgcg caagcaaacg gaacgagcct
     7021 tacgacgaac gatgttgaat ataaatgtaa tatccctctt gggcagactg ctggagaata
     7081 tactgctcct atcagctatc atctcatgac aacctaagat gaatgatatc ataggtattc
     7141 cttgcatttt tggaatggct tttttttcct gtgatatctt ttttttgtgc taaagttttt
     7201 tttaagagcg ttcattgttt tctgaagagg ttctcggttt atgactctat tacagagtga
     7261 ctttttgaca aataagcttt aaataccgtc tcaaccaaaa cgcatacctg acatgtgtta
     7321 acatgttgta aagtgactcg atatggagtg gaagaaggtg aaaaaaatgg agatggaaca
     7381 aaaaagaaaa ttattttcta ccctcgtagg aataacaata attacctcgt tggtgatcaa
     7441 tatctttctt attggcccga tcatcatgat tgggaaagcc gatcctggtg atatcccgca
     7501 tatctggcat aattcaacaa cgttaaatgt caccgtccta catttggaac caagaatcct
     7561 atggtatgat tatcaataca atcaatcagg aacctgggtc tcaaagctga acgcacaaag
     7621 tgatgtcaac aacagtgctg aataccgatt tattgtcaat atcagtagtg atcaaggctg
     7681 ggatgatatt gaatttgtcg atattaccgc atggtacgat caaggaaatg acagtagcat
     7741 ctataaccag accagaggtg gaaactggaa tttaaatctt caatatgaaa acaccacagg
     7801 aaccgctgta tggagaatgc tctggcccca taacggagag ttcatacagg gaacctatac
     7861 cgatagagtt ggaaaggacc cctatgggag tacaggtttt accgaatgtc ataatctatc
     7921 attctcgttt atccccagtt atcaggtgag atacgcccct ggtgacggag cgtgggatac
     7981 cacctataat acaacgactg ataatgaatc ttggaatttt attataacaa tcaccgatag
     8041 tggtgaagac gcaccaaatc ctataacaat ctggaccgca gacgagttcg gaatctactc
     8101 ctatagtgaa atcgtcagcg ccggatggcc aaccatcata ggtcaccctg gagaaaatgc
     8161 aacagcgaac agtaatatct ccctcatcac ccgctcaaat ggaaactatt cgcttacaac
     8221 agatgtcacc acattagttc atagaacatt tccaggagca acaatctctc gagagagaat
     8281 ctgggtgcgc ggtggtgatc tagatatctt taacaatttc acagcgaccc ctggtgggat
     8341 ctacttctac gggtcgctca gcgcgtacca tcttgcacag gcgaacggta ctgctctgac
     8401 caccgatgat gtccaatata aatgtgatat ccccctgggt caaatcgctg gagattacgt
     8461 cgccccaatc cggtatcatc tgacaacaac ataaagatac agtacatctc ttttttctct
     8521 tttttttcat ttcatttaac attttttgtc tgataagttg ttctgctccc atcctttttt
     8581 ccctttgatg agcaggaaag aataatcttc tcaaagtgta aagtaaagtg acatcgccaa
     8641 cgttttcaaa gccaacaaag taaaatgaag tgatcacatc cctatcattg ggatgaagag
     8701 ggatgggatc acttacacca gagatacgga aacgtataat taaagcttac aaaaaaggat
     8761 accgcatcaa agacatcgcg gctgtcttcg gagtcagccg ctgggtcata tggaaatgga
     8821 gaaaacgagc ataccaccga ggagtagaca gttatcgaag tcgc
//
LOCUS       DATMXJ010000173         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50374, whole genome shotgun sequence.
ACCESSION   DATMXJ010000173 DATMXJ010000000
VERSION     DATMXJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50374"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VN377_04455"
     CDS             complement(<1..1309)
                     /locus_tag="VN377_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HWR27671.1"
                     /translation="MKTDGVSWVYHMTTTLGIRREDKNRWEQRVPLIPEHILELQKKH
                     GIKTVVQPSAIRVFPDIDYQKVGAQVQESLTPSSVVFAVKEIPLHYFEKSKTYVFFSH
                     TIKGQKHNMPMLKKMMDLGCTLIDYERIVDAKGKRLVFFGRYAGLAGMVDTLWTFGRR
                     LHKEQINTPFNAIKQTIHYKDLAEIKKHLAVIGKEIKTKGLPTSLTPLIIGFTGYGHV
                     SIGAQEILDVLPVKEIHPKDIESICMNPSNKVIYKVVFKEEHMVEPTMAGKTFDLQEY
                     YQHPERYRSTFEQYIPSLSILMNCIFWSTQYPRLLTKQYTKELFSKKEKPCLKVIGDI
                     SADVNGAIEFTEKTTSPDNPVFVYNPLTDTIKDGYEGEGIVVMAVDNLPCELPKESSE
                     SFSEILSCFVPEIMNADFTAPDFETVTLPVEIKNAVILYRGKLT"
     gene            complement(1459..3471)
                     /locus_tag="VN377_04460"
     CDS             complement(1459..3471)
                     /locus_tag="VN377_04460"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HWR27672.1"
                     /translation="MEKKTTQITGILIVSLLVCASLVAVITLSPLDKGAYTYKLNTFQ
                     SYDDLRSFLEKRFAAGGNQGRYYDSPTVLFSKESGAANDGSALGGEAPPSYSTTNVQV
                     AGVDEPDIVKTDGTYLYVVANQTVYIIRAYPVFEATILAQIKITNVSISNIFIRGDRL
                     VVFGNTGGYYGYPLLERDMKVGAISQDGWWGGISQTAVNVYDLTERGAPALVKEIRID
                     GGYFDARLIGEYVYLVATEYTYNILYAVDETNYTLNVPQISIDNETQSISAEDIYYVD
                     VPELSDTMTHVISLNLDTNDVVEKSFMLGSSQTMYVSRNSIFLASLHYPYYPMLYRTG
                     TESTSADDQETTILHKISIDTGDVTYIAQGEVPGHILNQFSMDEHNGFFRIATTSGNN
                     WMEGSQTSNNIYILDDTLTQISKIENIAPGESIYAARFLGDRAYLVTFVQVDPLFTID
                     LSDPYNPRILGELKIPGYSEYLHPYDETHIIGIGKEVDPSIDADKVHTPGAVYYTAIL
                     GLKLALFDVSDIEHPVEEAKIVIGNRGTDSPALYDHKAFLFDREKELLVIPVSLYEIP
                     QDTLDSVNDSGITTFESDWGQFTFQGAYVYHLSLENGFDYQGRITHQTPEQMQQPEDY
                     YWYWGSSSTDITRTLFIGDVLYTISDSMVKMNNLGDLSEISSISLS"
     gene            3618..3893
                     /locus_tag="VN377_04465"
     CDS             3618..3893
                     /locus_tag="VN377_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27673.1"
                     /translation="MMGKRKVLKKTDVLSASEIGQYYYCSCAWHLQRCGYEPESPYLE
                     AGKQIHVALGDNIDGFETKIYYARWYAILGLLVLCIAVFLLFIEVIL"
     gene            3920..4426
                     /gene="cas4"
                     /locus_tag="VN377_04470"
     CDS             3920..4426
                     /gene="cas4"
                     /locus_tag="VN377_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HWR27674.1"
                     /translation="MIGAIVLIASAVWIRWSVQKKKKSYGIPDGLIVYSDLNVPAAPL
                     FSRRFRLTGKPDYILRKENQCVPVEVKSGKGPHPHQSQVLQLAAYCQILEDTSGEFVP
                     EGILVYNNVPYTIRFDPKLRFELESMMKTMRASLRSGVVKRNHHEPARCHHCSMKQYC
                     TDIIADVS"
BASE COUNT         1235 a          943 c         1135 g         1416 t
ORIGIN      
        1 gtgtaagctt ccctcgatac aggattaccg cgttttttat ctcaactggt aacgttactg
       61 tttcgaaatc tggtgcggtg aaatcagcgt tcatgatctc agggacgaaa catgagagga
      121 tttcactaaa cgattctgag gattcctttg gtagttcaca gggaaggtta tcgaccgcca
      181 ttacaacaat gccttcaccc tcatatccat ccttgatcgt atcggtgagg ggattgtaca
      241 caaagacagg gttatccggg gatgttgttt tctcagtaaa ctcgatcgca ccgttgacat
      301 ccgcactaat gtcaccaatc accttcagac atggtttttc tttcttacta aatagttctt
      361 tcgtatattg ttttgtaaga agacggggat attgagtgct ccagaagata caattcatga
      421 gtatggaaag tgaggggatg tactgttcaa aagttgagcg ataccgttca ggatgctggt
      481 agtactcttg caaatcgaat gtcttcccag ccatggttgg ttctaccatg tgttcttctt
      541 tgaacaccac cttatagatc actttattcg agggattcat gcagattgat tcgatgtcct
      601 ttggatgtat ctctttgaca gggagaacat ccaggatttc ttgcgccccg atcgagacat
      661 ggccatatcc ggtaaatccg atgatcaacg gtgtcaatga agtgggtaga cccttggttt
      721 tgatttcttt acctataacc gcaagatgtt tttttatttc agctaaatcc ttgtagtgga
      781 tcgtttgttt aatcgcatta aacggggtgt ttatctgttc tttgtgtaag cgtcttccaa
      841 acgtccataa tgtgtcgacc atgccagcaa gaccagcata tcttccaaaa aacaccaacc
      901 tttttccttt tgcatcgaca atccgttcat aatcaatgag agtacaaccc aaatccatca
      961 tttttttcag catcggcatg ttgtgttttt gtcctttgat ggtatgggag aaaaacacgt
     1021 aggtcttact tttctcaaag tagtgaagag ggatttcttt taccgcgaag acgacagagc
     1081 ttggcgtgag gctttcttgt acctgggcac cgaccttctg ataatcaata tctgggaaga
     1141 cgcggatagc tgagggttgt acgacggttt taatcccgtg tttcttttgt agttcaagga
     1201 tatgttctgg gatgaggggt actcgttgct cccagcggtt tttatcttct cgtcgtatgc
     1261 caagtgtcgt agtcatgtgg tacacccacg acaccccatc cgttttcatt taataggttt
     1321 tccgcagact ctttatcgag tagtatgaca taaaaattca tcttctgaaa gaaaaaaaag
     1381 gtgaggagag agggaagaag ggaggagaag gaggaaagga agggatgctt ccctctctca
     1441 tctgcgcttc agtatgaact acgataggct gatgctggat atttcgctga ggtccccaag
     1501 gttgttcatc tttaccattg agtcggagat cgtatacagg acatcaccaa tgaacaaggt
     1561 tcgggtgata tcggtgctgg aggagcccca ataccaatag tagtcttctg gttgttgcat
     1621 ctgttctggg gtttgatggg tgatccgccc ttgataatca aagccgttct caaggctgag
     1681 gtggtagacg tacgcacctt ggaaggtgaa ctgtccccag tctgattcaa acgttgtgat
     1741 accgctgtca ttgactgaat ctagggtatc ctgaggtatc tcgtagagac tcactgggat
     1801 gacaaggagt tctttttctc ggtcaaagag gaatgctttg tggtcataga gagctgggct
     1861 gtcggttccc cggtttccaa tgacgatttt cgcttcctcg acagggtgtt cgatatcact
     1921 gacatcgaag agtgcgagtt tcaacccaag gatagctgta taatacactg cacctggcgt
     1981 atgtactttg tctgcatcga tgctgggatc gacttctttt ccgataccga tgatatgtgt
     2041 ctcatcgtag ggatggaggt attcggagta cccaggtatc ttcagttcgc caaggattcg
     2101 tgggttatag gggtctgaga gatcaatggt gaagaggggg tcaacctgta cgaaggtgac
     2161 gaggtacgcg cggtctccaa ggaaccgggc tgcgtagatg gattcacctg gtgcgatgtt
     2221 ctctattttt gagatctgtg tgagggtgtc gtcaaggatg taaatgttgt tactggtctg
     2281 ggagccttcc atccagttgt ttcctgaggt ggtcgcgatt cggaagaacc cgttatgttc
     2341 atccatggag aactggttca gaatatgacc agggacttcc ccttgtgcga tgtaggtgac
     2401 atcaccagtg tcaatggaga tcttatgcag gatggttgtt tcttggtcat cagctgatgt
     2461 gctttcagtt cctgtcctgt ataacatggg gtagtaagga taatggagtg atgcaaggaa
     2521 gatgctgttt cgtgacacat acatggtttg ggagcttcct agcatgaagc ttttctccac
     2581 gacatcatta gtgtcaaggt tcaatgatat cacatgggtc atagtgtctg aaagctcagg
     2641 gacatcaaca tagtagatgt cctctgcgga gatgctttgg gtttcgttat caatactgat
     2701 ttgtggaaca tttagtgtat agttggtttc atcaacagca tagaggatgt tataggtgta
     2761 ttctgttgcg accaggtaaa cgtattcccc gatcagacgg gcatcgaagt agccaccatc
     2821 gatccgtatc tcctttacca gagcgggggc acctcgttcg gtaaggtcgt agacgttgac
     2881 tgcggtctgt gagataccgc cccaccagcc atcttggctg atggcaccca ctttcatatc
     2941 acgttcgaga agaggatagc cataataacc accagtgttc ccaaagacca ccaagcggtc
     3001 accacggatg aagatgttac tgatggagac gttcgttatt tttatttgag caaggatcgt
     3061 tgcttcgaat actgggtagg ctctgatgat ataaacggtc tgatttgcga ctacatacag
     3121 gtaggtccca tcggttttta cgatatcagg ttcgtccacg ccagcgacct gtacattggt
     3181 cgttgaataa gaagggggcg cctcaccacc taaggctgat ccatcatttg cggctcctga
     3241 ttcttttgaa aacaagacag taggtgaatc gtagtaacgt ccttggtttc cccctgctgc
     3301 aaagcgtttt tccaggaagg aacggagatc atcatacgat tggaacgtgt tcagtttata
     3361 ggtataggct cctttgtcta gcggcgaaag agtaatgact gccacaagac ttgcgcacac
     3421 cagcagactt acaattaaaa ttcctgttat ttgagtggtt ttcttttcca tagcgatcac
     3481 atctgtgttt aattcaggtt atatctgtca gactttatat agtgtctgaa aacgtttggt
     3541 aaaaaccaaa caactgaagc gtattgtttc agtcatcccc catatagcta taaatagcga
     3601 tattccatca tagcggaatg atgggaaaac gaaaggttct gaagaagacc gacgtgttaa
     3661 gcgcatcaga gatcggccag tattactatt gttcctgcgc atggcacctt caacggtgcg
     3721 gctatgaacc agagtcacca tatcttgagg caggaaaaca gatccatgtg gcgttgggtg
     3781 acaatatcga tggctttgag acaaagatct actatgcacg atggtacgca atcctgggtc
     3841 tcctggtgtt gtgtatcgct gtttttctcc ttttcattga ggtgatccta tagatttctt
     3901 ggttctgagc atcattctga tgattggtgc aattgttctt attgcgtccg cagtatggat
     3961 acgatggtcc gtgcagaaga aaaaaaaatc atatggaatc cccgatggat taatcgtgta
     4021 ttcagatctg aatgtgcctg ctgcaccgct cttctcaaga cgtttccggc tcactggaaa
     4081 accagattac atcctaagaa aagaaaatca atgtgttccc gtggaagtca aatcaggaaa
     4141 aggaccacat ccacatcaga gtcaggtgct gcagcttgct gcgtattgcc agatccttga
     4201 ggatacttca ggggaatttg ttccagaggg gattctcgta tataataatg taccgtatac
     4261 aatccgtttt gatccgaaat tacgttttga actcgaatca atgatgaaga ccatgcgtgc
     4321 gtcgctacga agcggtgtcg tgaagcggaa tcatcacgaa ccggcaaggt gtcatcattg
     4381 ctcgatgaaa cagtattgta cagatattat agcagatgtt tcctaattcc ataaaaaata
     4441 atatagctta tattacaaaa aaattaggag aacaggtata ggaaaaggtt attaaaaaat
     4501 tatattttat tttagcaata ataatatggt attcaacacc cattttggga ttgtttagtc
     4561 ttttaggagt tatatttttt atattaggat ttttaataat tttaaggggt agagaaataa
     4621 aacattaaaa ccatgtcaat gcttctgatt tgatatttca ttaaggacag taataaacat
     4681 tcacttttga tggatgaact ggatacgcta tagtaaatag aactatctt
//
LOCUS       DATMXJ010000174         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_23885, whole genome shotgun sequence.
ACCESSION   DATMXJ010000174 DATMXJ010000000
VERSION     DATMXJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23885"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..605
                     /locus_tag="VN377_04475"
     CDS             465..605
                     /locus_tag="VN377_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27675.1"
                     /translation="MQNKSKDTQKRQALNCATCGKTFSQDEKTVSYDFERYYCFHCDD
                     QR"
     gene            730..1842
                     /locus_tag="VN377_04480"
     CDS             730..1842
                     /locus_tag="VN377_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWR27676.1"
                     /translation="MELTEIYQEKLSAKTSDGWELRLYHYPPVGKDVARFPVILCHGL
                     AGNKNSCDFGEPGTPEWERYSLAAYLSQQHPENEAVFDVWVPELRGNGHPTFDPRHHP
                     ERYRWSVDDYIDKDVPAIIRRVQEWYKEKKHGATPVFWVGKSMGGMIAYAYGQTKEGQ
                     ENLQGVVTLGSPVVFGKTSVFLEFLTRVTPRNVSIPIRIAELLERSGELTSHFRGMGV
                     NLENVDPAVLQTYLQVGFSNVLSSKVLSQFSLFFKHMTFCRYPRYPWMYDTIGRIPGM
                     KKVFTPYSYTENLHRFTAPLLVIAGGHDKMAPKTDMQYVKDHVGSTDVSYLEFSKDAG
                     YRADYGHIDLNLGLHAREEVYPKIYDWLVERSKTSM"
     gene            1775..>3050
                     /locus_tag="VN377_04485"
     CDS             1775..>3050
                     /locus_tag="VN377_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWR27677.1"
                     /translation="MPAKRCTRRSTTGWWNEAKQACKYLEKHDTIKPVYLFSVLMTVY
                     SHSRLSCYEQCPQKFKLQYIDKLETEEEQTVEAFLGIRVHEALEKLYRDLAHQKINTL
                     EELLTFFTSEWNTNWTDDIVIVKEEYSPENYLNMGRKYLTDYYQRYHPFRQGKTVALE
                     ELIRITLDPEDKYKLQGYIDRLTETRDGYYEIHDYKTNSRLPLADYIRSDRQLALYMI
                     GVKNQYPDVKDVKLIWHFLKFDKEIDSTRTDTELSQLKKDTIQLIETIENDERYEAHP
                     GYLCDWCEYQSCCPQWSHLHLIKEKPANEYLKDSGVILVNRYAEVKSAQKLANLQWDA
                     ELEKLEEALSSFAEKEHVEVVFGSKNKVRVTDSEKYSFPSKNSKEREQLEKVLQKYGR
                     LQDVSQLDTSALGKILQEKQWEPEVLDALHKYI"
BASE COUNT          867 a          629 c          757 g          797 t
ORIGIN      
        1 ggtgattgtt gttcgagagc cttgtgggta gccgagtgat tccgaaagta taattctctg
       61 cgttctgtgg ttttatgaaa cattgtgacg atggtatgaa ttacttgtgt acttttttcc
      121 aaaaaacaac ggaaaaaaag gaattttttc aaaggattaa cagatagata gatttattta
      181 tttgatatct atattaatat tgggtttggg ctctttagaa tgtttttttt gcattctttc
      241 ctcccagact tttaagccca gacccagctt ctcagggaga ggggattctc catcgagggt
      301 gataattgtc actttttaag aaacagggtt tttgattttt ttcggatcgt tctccggaat
      361 gaacttttgt tgacagtctt gaaacatcag atgaaaaggg atatatactg catgttcata
      421 catgaggaac ttcaaaccga gaaccaacat ggatggtgag ggagatgcaa aacaaatcaa
      481 aagacactca gaaacgacaa gcattgaatt gcgcgacttg cggaaagacc tttagtcagg
      541 atgaaaaaac agtcagttac gatttcgaac ggtactattg tttccattgt gacgatcagc
      601 gatagctctc tataagtact ttttttaata aaaaatacaa cactacagta ctgatgattt
      661 tctatcgatt tcttttttcc gaggattgga agggagagtt tttctatcga gatgtgttct
      721 cgtcattgta tggaacttac ggagatttat caggagaaac tatctgcaaa gacctctgat
      781 ggatgggagc ttcgtctcta ccattatccc cctgtcggga aggacgttgc caggtttcct
      841 gttattttat gccatggtct tgctgggaac aagaacagtt gtgattttgg cgaaccagga
      901 acgccggagt gggaacggta cagtcttgca gcgtacctct cacagcaaca tccagagaat
      961 gaagctgtct ttgatgtctg ggtaccggag ctccgaggta acgggcatcc tacgtttgac
     1021 ccacggcatc acccggagcg gtaccgctgg tctgtggatg attacatcga caaagacgtc
     1081 ccagcgatca tcagacgtgt acaggaatgg tacaaggaaa agaaacatgg tgcaacaccg
     1141 gttttctggg tgggaaaaag catgggcggg atgatcgcgt acgcctatgg gcaaacgaag
     1201 gaaggtcagg agaaccttca gggcgtggtc acgcttggct ctccggttgt gttcgggaaa
     1261 accagcgtgt tccttgagtt tctcacacgg gtcactccgc ggaatgtttc catcccgatc
     1321 cggattgccg agcttctgga acgatccggt gaattgacca gtcatttcag gggcatgggg
     1381 gtgaaccttg agaacgtcga tcctgcggtg ctacaaacct acctgcaggt cgggttttcc
     1441 aatgtcctat cctctaaggt tctctcccag ttctcgttgt tctttaagca tatgacgttc
     1501 tgtcggtatc cgcgatatcc ctggatgtat gatacgatag ggcgcatccc tggtatgaaa
     1561 aaagtgttca ccccgtatag ctatacagag aatctgcatc ggttcaccgc cccgttgctg
     1621 gtgatcgcgg gcggacatga taaaatggca cccaagaccg acatgcagta tgtgaaagac
     1681 catgtgggca gcactgatgt ttcctatctt gagttctcta aagacgcggg ctatcgtgct
     1741 gattatggac atattgatct gaatctaggt ctgcatgccc gcgaagaggt gtacccgaag
     1801 atctacgact ggttggtgga acgaagcaaa acaagcatgt aaatatttag aaaaacacga
     1861 taccattaaa ccagtctatt tgttctctgt tcttatgacg gtgtactctc attctcgcct
     1921 cagctgttac gagcagtgtc ctcagaaatt caagttgcag tatatcgata agcttgaaac
     1981 cgaggaagag cagaccgtcg aagcgttcct tggcatccgg gtccatgagg cgctcgagaa
     2041 actgtatcgt gatctcgccc atcaaaaaat aaataccctg gaagaactgc tcactttttt
     2101 cacaagcgaa tggaacacga actggaccga tgacatcgtt atcgtcaagg aagaatacag
     2161 tcctgagaac tacttgaaca tgggcaggaa gtatctgacg gattattatc agcgatatca
     2221 tccgttccga caaggaaaga ccgttgcttt ggaagagtta atccggatca ccctcgaccc
     2281 agaggacaag tataaactcc aaggatatat cgaccggttg accgagacaa gggatggata
     2341 ttatgagatc catgactata agacaaactc acggttgccg ctggctgact atatccgttc
     2401 ggaccgtcag cttgcactgt acatgatcgg ggtgaagaac caatatcctg atgtgaagga
     2461 tgtgaagtta atctggcatt tcttgaagtt cgataaagag attgattcca ccaggactga
     2521 tacagagctt tcacaactaa aaaaagatac tattcaactc atcgaaacaa tcgaaaacga
     2581 cgaacggtat gaggcgcacc ccgggtatct ctgcgactgg tgcgagtatc aatcctgctg
     2641 tccccaatgg tcccatttgc acttgatcaa agagaaacca gcgaacgaat atctgaagga
     2701 ttcaggcgtt atcctggtga atcgatacgc cgaggtcaaa tcagctcaga agcttgcgaa
     2761 tctgcagtgg gacgcagaat tggaaaaact tgaggaggca ttgagttcgt ttgcagaaaa
     2821 agaacacgta gaggtggtgt tcgggtcgaa gaacaaggta cgagtcacag acagtgagaa
     2881 atattcgttt ccaagtaaga atagtaaaga acgagaacaa ttggagaaag tgctgcagaa
     2941 atatgggaga ctccaggatg tcagtcagct tgatacatca gcgctaggga agatccttca
     3001 agagaaacaa tgggaaccgg aggttctgga tgctttacat aaatatatag
//
LOCUS       DATMXJ010000175         3733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_13997, whole genome shotgun sequence.
ACCESSION   DATMXJ010000175 DATMXJ010000000
VERSION     DATMXJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13997"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..452)
                     /locus_tag="VN377_04490"
     CDS             complement(276..452)
                     /locus_tag="VN377_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HWR27678.1"
                     /translation="MEKRDRSDRGGRDYGAPREMHDVTCADCGKETQVPFAPDGNRPV
                     YCRDCYQKHRPKRF"
     gene            623..2572
                     /locus_tag="VN377_04495"
     CDS             623..2572
                     /locus_tag="VN377_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27679.1"
                     /translation="MKKRGFIVIISILATLSMVVGSIQAGTAQKNISLVTHLQTKTIT
                     YQFSQPSIQENNQYATIALAEATGYRNIAGEPILPIIMKTIELPFGTTIKDIHGSFSS
                     QPYIQLTQKITPAVQPVPFITEDLIRQPSENPEIYTHDQVFPETWYSSRLTGGLNKQN
                     ILTTFLTIQINPVRYNPAQYTIQPLDAITIDITYELPSQTFSSSTDTYDMIIICKDSY
                     ARLLEPLVAHKNSHGIQTKLIPISSIYAGTFFPAEGRDKPEQIKYFIKNAKETWNITY
                     VMLVGNYAQVPTRYANLETDTGGLYEELRFVSDLYYADLYTANGSFSSWDTDNDGLYA
                     EWPYPEGHPQEDLVDLVPDVHVARLACMFKSEVKTMVDKIITYENTAGGTDWFKRIVV
                     IGGDTFDKSIEGGTDYNEGEEATAKALEYMPQFIPVRLWASLGNLSLETIQDEISKGC
                     GFLYFSGHGSPKSWATHNNGDYKNWTGQYKNTDMPDLTNKDMLPIMIVGGCHNSELDT
                     APKNLLLGFLKEKLNYFRMNETWFGSYFKYNYALECWSWVLVKVKGGAIASTGSSGFG
                     GVNVGDYNQDGIPDCTQGLDGWFELEFFRLYNQENITILGQTYDQVLTNYVQNFPVDT
                     YRYDAKILETHILLGDPSLQIGGYQ"
     gene            complement(2612..2965)
                     /locus_tag="VN377_04500"
     CDS             complement(2612..2965)
                     /locus_tag="VN377_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27680.1"
                     /translation="MNPLFVKALKNGFDMSREDAVALAQTVQKVFKGVKEVEDMSLHK
                     DIRSIFYDLHQKNLLCLRREEVKEKGKAIRKYYWSFNSDGIRIEAYRRPIEEPQYEIY
                     KKIPEDAWLLHSCNT"
     gene            complement(3157..3318)
                     /locus_tag="VN377_04505"
     CDS             complement(3157..3318)
                     /locus_tag="VN377_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27681.1"
                     /translation="MNKTLIVLGAGIWGVLILLRFVLSGVESNTFQLLNILEIVAFVI
                     TIVGIVKRD"
     gene            3458..>3733
                     /locus_tag="VN377_04510"
     CDS             3458..>3733
                     /locus_tag="VN377_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWR27682.1"
                     /translation="MTYYSFHDSEKPVRIGKIICLARTYKEHAREMNANVTEDPLLFL
                     KPTSSIIFDHGTIIIPKRSHCLHHEVELGIIIGKKGKNIRQENAMHHV"
BASE COUNT         1194 a          931 c          729 g          879 t
ORIGIN      
        1 accaattaat gcagtggttc cgagtgcacc gataccaggg agccgaagga gtttcgcgta
       61 cgcagcaacc ttcttcatag agatgtgtta aagtctgact ctttttgagc ttttcccatt
      121 tatatgtctt cagattacta aaatacttcg agaaggataa aaattttata aataatacct
      181 taccaccaga taaaaaagaa taaaaagtag tttttccttc ttcagagaag aaaaagaaaa
      241 aaccacgttc gaacgatctt agatatatat ctactttaga atctctttgg ccgatgtttc
      301 tgataacaat ccctacagta gacgggcctg ttaccatctg gcgcgaacgg aacctgggtt
      361 tccttaccgc aatctgcaca agtaacatcg tgcatttcac gtggtgcacc ataatcccga
      421 ccaccacgat cggatctatc tcttttttcc attattatat acttcctttt ttatattcgg
      481 ttcattgttt ccttcgaacc gctggactgg catatagaga agaggtattt aactttattg
      541 atgacaacaa ggtggaaaaa cgacaaaagc tatttataac atgacaccgt taaatacagt
      601 agagaatacg ggggtaacac caatgaagaa aagagggttc atcgtcatca tcagcatact
      661 cgcaacactc agtatggtcg ttgggagtat ccaggcagga accgcacaaa aaaacatctc
      721 actagtaaca catctgcaaa ccaaaaccat cacctatcaa ttttcccaac cgtcgataca
      781 agaaaacaac cagtatgcga ccatcgcttt ggcagaggcg accggatacc ggaatatcgc
      841 tggcgaacca atcctaccca tcatcatgaa aaccattgaa ttaccattcg gtacaacaat
      901 aaaagacata catggcagct tcagcagcca accatacatc cagctcaccc agaagatcac
      961 acccgcggta caaccagtac cgttcatcac cgaggatctg atcagacaac cctcggaaaa
     1021 cccagagatc tacacgcacg accaagtctt cccagagacc tggtactcct cccgactcac
     1081 cggaggactc aacaaacaga acatcctgac aacgtttctg accatccaaa tcaacccagt
     1141 gcgatataac ccggcccaat acacgataca acccctggac gctattacta ttgatattac
     1201 ctacgaacta ccatctcaga cgttttcttc atccactgat acgtatgata tgatcatcat
     1261 ctgcaaagat tcctacgcgc gattactcga gccattagtg gctcataaaa acagccatgg
     1321 gatccagaca aaactcatcc ctatttccag catctatgca ggaacctttt tccccgctga
     1381 aggcagggac aaaccagaac aaataaaata tttcataaaa aacgcaaaag aaacctggaa
     1441 catcacatat gtgatgctgg tcggcaacta cgcacaagta ccaacaaggt acgccaacct
     1501 tgagactgac acaggcggat tgtacgaaga actgagattc gtctctgacc tgtactacgc
     1561 tgacctctac accgcaaacg gaagcttctc cagctgggat actgataacg atggcctcta
     1621 cgctgaatgg ccgtatccag agggtcatcc tcaggaggat cttgtcgacc tggtcccaga
     1681 cgtccatgtc gccagattag cctgcatgtt caaatcagaa gtaaaaacca tggttgataa
     1741 gatcatcacc tatgagaaca ccgcaggagg gacagactgg ttcaaacgga tagtggtcat
     1801 cggcggcgac acctttgaca aaagcataga ggggggaacc gattacaacg aaggagagga
     1861 ggcgacagcc aaagccttag aatacatgcc acaattcatt ccggtcagac tctgggcatc
     1921 cctagggaat ttatccctcg aaacgatcca ggatgaaatc agcaaaggat gtggatttct
     1981 ctacttctcc ggccatggaa gcccgaaatc atgggcgacc cacaacaatg gggattacaa
     2041 gaactggacg ggacagtaca aaaacacgga catgccagac ctcaccaaca aggacatgct
     2101 cccgatcatg atcgtcggcg gctgccataa cagcgaactc gataccgcac caaaaaacct
     2161 tctcctagga ttcctcaaag aaaaactcaa ctacttcagg atgaacgaaa cctggtttgg
     2221 atcatacttt aaatacaact atgccctgga atgctggagc tgggtgttgg taaaggtcaa
     2281 aggaggcgcc atcgcatcca cgggaagcag cggatttgga ggagtgaacg tcggggacta
     2341 caaccaggat ggcatcccgg actgcaccca gggacttgat ggatggttcg agctagagtt
     2401 cttccgctta tacaaccagg agaacatcac gatcctcggg cagacctatg accaggtgct
     2461 caccaattat gtccaaaact tcccggtaga cacctatcgg tacgatgcaa agatcctcga
     2521 gacacacatc ttacttggtg acccaagtct tcaaatcggt gggtaccaat aaaaaaaaaa
     2581 ttgaaaaaaa aaaggaaaac aagagacaat tctaggtgtt gcacgaatgc aacagccagg
     2641 cgtcctcagg gatcttctta tagatctcgt actgaggctc ctcgataggt ctccgatacg
     2701 cttctatacg gatcccatca gaattgaaag accaataata cttccggatg gctttaccct
     2761 tttcttttac ctcttcccgt ctgagacaga ggaggttttt ctgatgtaaa tcgtagaaaa
     2821 tcgaacggat gtccttatga agcgacatat cctcaacctc tttcacaccc ttgaacactt
     2881 tttggactgt ttgggctaaa gcaactgcat cttctcggct catatcaaac ccgtttttca
     2941 atgccttcac aaataaggga ttcatctttt ctcaccgaat accattctgt tgctttcatt
     3001 attaaggaat tgttgtacac cagataccag ggttctgtac gagcgttacc gaaacccgtt
     3061 tttaccacac cgccggaaag aaaaaaacta tgaaaaactc tctgccaatc atctaaaaaa
     3121 aggaataatt ttcgatacgg atacgataga ttctagttaa tctcgcttga cgataccgac
     3181 gatcgtgatg acgaacgcaa caatctcaag gatatttaaa agctgaaagg tatttgattc
     3241 aacgccggaa aggacgaatc ttaaaaggat aaggacaccc cagattccag cacctaatac
     3301 gatcaaggtt ttattcatct gttactctcc cgaccattcg gaaccttcaa ggaagatatg
     3361 ataagatgtc attataatac ttgtcctttt tcgtttgaaa tagagcatca aaaatcttca
     3421 caaaatctat taacacctga ctcgatgttg accccatatg acgtattatt cgtttcacga
     3481 ttcagaaaaa cctgtacgca ttggaaaaat catctgcctc gccagaacat acaaagaaca
     3541 cgcacgagaa atgaatgcca atgtcacaga agatccctta ctcttcctca aaccaacaag
     3601 ttccattatt ttcgatcatg gtacaatcat catcccgaaa cgatcgcact gcttgcacca
     3661 tgaggttgag cttggaatca tcatcggaaa aaaaggaaag aacatccgac aagaaaacgc
     3721 gatgcaccat gtg
//
LOCUS       DATMXJ010000176        10236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_45564, whole genome shotgun sequence.
ACCESSION   DATMXJ010000176 DATMXJ010000000
VERSION     DATMXJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45564"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VN377_04515"
     CDS             complement(<1..967)
                     /locus_tag="VN377_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWR27683.1"
                     /translation="MVKKELLKNEQIERMLSPHPLSFMKLQTLCIFVIVWGIVVWWLT
                     EFSEYAGIFSGNIWYPLILWGLVLLLVGVIASLVSIRWTIFFLYLGVFAGALGFIFWQ
                     HWESYIELFIPLYSIAVSIVGFLIVELYRRSHKYIITNLRIIFKGGIFTKRERTIRYD
                     KIADIDAKQGVLGQIFGFGTIIPVSQSGFGLGSDKTMAAGGVEVGSTKARFFGFAGGG
                     KEVQTPIARSYYELHGVYPYKDVKQLVENLVQSHVPTQYQKEQSEFQKQQVDIQTQMR
                     DLLHKHVSDKPRGKMMRQPVEEEEEEESEEEAVEKVSRKKPVAPEE"
     gene            complement(939..2009)
                     /locus_tag="VN377_04520"
     CDS             complement(939..2009)
                     /locus_tag="VN377_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27684.1"
                     /translation="MANVPLNIDVLLVAALAPILGVAAFWFLQMFFIELQKRMLSRLR
                     HRHEAFCRFTNYIGLLFQTISQALGYTVTRSGIAAFQVSVNYGKVEPKKEKKGVFEWV
                     ARSFLLFGPFFIPSGLVLVASYFIIGKGFVYSTPVEYTFVESLVGFWGSMSTFANEWL
                     TFLANIDLFNPLHIGFLFVLLFFGLGIRPSYIGQERKEKIDILYDLRHIKNHLVEKPL
                     YIVVIILILFVFYLLSLFLNPVVYIGLFTIFGWMSLTAIIAILLTFPVLLMLRATDEI
                     RPHWKLIPLLTLVVSYVVARVLFIYFPAKDVERVSLLVMLLSTILITIALIKYKRTNR
                     FKTATKMKHVRVADGKKRTSKK"
     gene            complement(2053..2469)
                     /locus_tag="VN377_04525"
     CDS             complement(2053..2469)
                     /locus_tag="VN377_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HWR27685.1"
                     /translation="MGLFRRKTTLPIPYPWKITRKCLDLISESSKSNYPQEFGALLRV
                     DRVEKNTIIEVVLLPGTISGDSHAIFQLHMLPVDYSIVGTVHSHPSSIARPSNADLDL
                     FGHFGKIHIIVGVSAYGDVSWQAYDYAGRGVVIEII"
     gene            complement(2473..3291)
                     /locus_tag="VN377_04530"
     CDS             complement(2473..3291)
                     /locus_tag="VN377_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWR27686.1"
                     /translation="MTTNWGLVCNPGVKRTVTLAKDVAEFLQQHGTVLVEEAIASEIQ
                     YKGSSFKEINKNADIVVTIGGDGTILMAMSEIEKPVFAINSGGIGFLSEVESKYAMEG
                     LKQVIAGKYNVEERAKLKITLDGKRLPDATNEVTIQTARIAKILYLKLFVDDELIESM
                     GADGVIIATPTGSTSYSLSVGGPIMDPTVNAMIIAPLAAFKLSARPWIVPLDRKVGVH
                     LLPKSKETNLVIDGRFQTPVTQESTISVTGSEKKARFIRFGESFYQMVRLKLMR"
     gene            complement(3294..4484)
                     /locus_tag="VN377_04535"
     CDS             complement(3294..4484)
                     /locus_tag="VN377_04535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWR27687.1"
                     /translation="MHYDAVVVGSGPAGSVTARFAAEAGAKVLIIERRAEVGVPVLCG
                     EGISQKVDNFKVLEGKRWIATKMKGARIFSPNGTHVTLAAEYAGDETGYVVYRDIFDQ
                     ELARGAVKKGAELMLNTLAVDLLKKNGKITGITAQQFDETLDIEADVIVGADGVESRV
                     GRWAGIKTTLKPHDLETCVQYTLTNIDWDSPYCDFFLGKHVAPGGYVWVFPKGKDVAN
                     VGIGVLASLSEAGKAKQYLDKFIAAQPALKKGKPLRILTGADPVAEPIQSITDNLLLV
                     GDAARQVDPITGGGLMHSIEAGRYAGETIGKAVEKQRFDSEMLSEYETRWKNAFGKKL
                     HRNYIAKEIMLDMTDKTLDMLADSLKDYKFEEFSTLSIIKALVMKHPSLLVKLTPLMK
                     LARA"
     gene            complement(4486..4674)
                     /locus_tag="VN377_04540"
     CDS             complement(4486..4674)
                     /locus_tag="VN377_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR27688.1"
                     /translation="MAKHKAVVKKEKCLACGGCISVCPQDAILMISSKAVVDNELCNS
                     CSICVKTCPIGAITWEGT"
     gene            complement(4661..5233)
                     /locus_tag="VN377_04545"
     CDS             complement(4661..5233)
                     /locus_tag="VN377_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HWR27689.1"
                     /translation="MKRQIRILGIDDAPFTFTEKYSTIIGVVMRGGEYIEGVLRNQVT
                     IDGTEATEICKQMIQRSRYRRQLKTVMIDGACLGGFNVVDIDDLSESTHLPVITITRD
                     KPDQRKIKQALQKNFKDWQQRWNLLQKGSLHRIPTQHNPIYIKCTGISLEEAKEIINI
                     STIRGVIPEPIRVAHLIASGITRGESYGKA"
     gene            complement(5514..5747)
                     /locus_tag="VN377_04550"
     CDS             complement(5514..5747)
                     /locus_tag="VN377_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWR27690.1"
                     /translation="MAIGFVLINVAPAHEHEVYNKLSKIPQIVELHPLFGEYDLIAKI
                     DANDFEELGTIIVNKIRAINGVLDTKTLTGLTF"
     gene            complement(5826..5948)
                     /locus_tag="VN377_04555"
     CDS             complement(5826..5948)
                     /locus_tag="VN377_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27691.1"
                     /translation="MSLYCSVCGDELEPEEEMEGICESCKLAASGQVSTDEVEP"
     gene            6259..7236
                     /locus_tag="VN377_04560"
     CDS             6259..7236
                     /locus_tag="VN377_04560"
                     /inference="COORDINATES: protein motif:HMM:NF004996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWR27692.1"
                     /translation="MDLTGLQTEDEPPPLNGVGDTLFSRARNLERVLGFGNIFLKFEG
                     GNPTGTQKDRISQYHVKKAMQQGYDTITVGTCGNYGVSLAYFAKLAGLKAKILIPEQY
                     YVAPKRKEELQSMNAEILTIDGKYEDAVEESRRLSRMEHVYDANPGNGNEGWRGYMSI
                     AYEIYRQLGRTPTAVAAPVGNGTTLVGIYHGFKNLLDHNIIDHMPYLVGASTPGGNPI
                     IKSFKTGASKTQDLPPSEIKESSINEPLIAYHSYDGDDALQAIYQTNGWADYASDSCM
                     MKFHTMLRDLEGLNVLPASASPIEALIKFKEHRVLNSDYVIVLTGRKFR"
     gene            7263..8318
                     /locus_tag="VN377_04565"
     CDS             7263..8318
                     /locus_tag="VN377_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWR27693.1"
                     /translation="MAKMRALVYADRLYNCADGKTAHGLVRFSKKFDIVSVVDSTLPI
                     GDAAEFLDGKKKGIPLLNDLDDALSSTHPDTFIIGAVSEGGVLPKGYDKAVDWALSKE
                     LNVVSGLHQFLSDDPRFIKCAAEHHCTITDVRKIFRDHKRFYTGEITQVPSIRIALIG
                     TDSAIGKRTLAVLLVNELQKRGRTADMIFTGQTGWLQGWPHGVVLDAMINDFVSGGIE
                     GAIIDSWNDLHPEFMIIEGQGSLVHPFFPGGFEILAAGQIQGFILVDAPKRPHLDGFP
                     DYPMPDPKRVVRIAEMLTEKRLIGVGINHEKMNTSDITRAKTMYQKMFKVPVSEPLSE
                     GVGVLADALEVLSHGKK"
     gene            8305..9738
                     /locus_tag="VN377_04570"
     CDS             8305..9738
                     /locus_tag="VN377_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27694.1"
                     /translation="MAKNNLLCLSSISLSDPMIEKNVARAEIIVIESDGTESRFELMN
                     KYEHELMQSHLPLLRLAFCMPLLNYGLFSKKIKLNFPISKADISLLNDLNVIFSRDIF
                     VNKIAQKTNPYILPEFFPDQEKIGPHDGDPTAVIQPKQIIDDVPISENMDAMKSGVLS
                     SGGKDSLLTYGLLKELGSTVYPLYMNESGGHWRTALTAYQNHKKTEPNTQRVWTNVDR
                     FYSFMLDHLRFIRSDHRKIWHDTYPIRLCIFPFYVFSLLPIFVEERIGNLLLGSEFDD
                     LRYEVQYKGIKHYFGVYDQHQDYDVRMNQWYEKRIPGLYQWSALRNISGLVDQNILVR
                     RYPALAKLQRSCHSCHIKNGVVYPCGTCSKCLGILLYLLANDLDPTLMKYRRKDIEDF
                     YINVGVSSLKLDKDEKDQSFYLLKRQGSIPKVQCVDHVEKIHVHESTCDPNLYPRQFR
                     NGLLKILEEYTTGYCSLKNKKWVPTNK"
     gene            complement(9746..>10236)
                     /locus_tag="VN377_04575"
     CDS             complement(9746..>10236)
                     /locus_tag="VN377_04575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWR27695.1"
                     /translation="FMYPTTMQQTIHIRTASPDDFERIVALEKICFPKELAYTRRQLR
                     HLLMKANSTVLVEKTSTVLRGFIIILYRRGTKVAGVETINVDPMYRKKGIGQRLLTAA
                     EERIRKKGMHKIRLEVAIINQAAIMLYEHAGFQKTMLLKKYYNYDHEGSRDAYRMVKE
                     LE"
BASE COUNT         2821 a         2475 c         2156 g         2784 t
ORIGIN      
        1 cttcctcagg tgcaaccggt tttttcctag aaactttttc tactgcttcc tcctcagatt
       61 cctcttcctc ttcttcttcg accggttgtc tcatcatctt tccccttggt ttatcactca
      121 catgcttatg aaggagatca cgcatctgcg tctgaatatc cacctgttgc ttctgaaact
      181 cactttgttc cttctgatac tgtgtaggga catgagattg aaccaggttt tcaacaagtt
      241 gtttcacatc cttatacgga tacacaccat gcaactcgta ataacttctt gcaatcggtg
      301 tctggacctc ttttccgcca ccagcaaaac caaagaaccg agctttcgtc gagccgactt
      361 ccacaccacc agcagccatg gtcttatcac tccccaaccc aaaaccagat tgactcactg
      421 ggatgatcgt cccaaaccca aaaatctgac cgaggactcc ttgtttcgca tcaatatcag
      481 caatcttatc ataccggatc gtacgctccc gtttcgtaaa gataccaccc ttgaaaataa
      541 tacgcaggtt cgtgatgata tatttatgtg accggcgata gagttcgacg atcaaaaaac
      601 caacaataga aacagcaatt gagtataagg gaatgaaaag ctcaatgtag ctctcccaat
      661 gctgccagaa aatgaaacct aatgcacctg caaagacacc aaggtatagg aaaaaaatag
      721 tccatcgtat cgaaacaagc gaggctatca caccgacgag aagcaacacg agcccccaaa
      781 ggatcagagg gtaccagatg ttccccgaaa agatgcctgc atactctgag aattccgtca
      841 gccaccacac cacgattccc cacacgatga caaagatgca gagcgtttgt aatttcataa
      901 acgacaaagg atgaggtgag agcatccgtt ctatctgctc attttttaga agttcttttt
      961 ttaccatccg caaccctcac atgcttcatc tttgtcgcgg ttttaaatct attggtcctc
     1021 ttatatttta tcaaggcaat ggtgatgaga atcgtcgaga gcagcatcac cagaagagaa
     1081 acacgttcaa catctttcgc ggggaaatag atgaaaagca cccgagcaac aacataggaa
     1141 accactaaag tcagaagtgg aattagtttc caatgtggtc ggatttcatc ggtcgccctt
     1201 aacatgagaa gcacgggaaa tgtaaggagt attgcgatga tcgcggtcag cgacatccaa
     1261 ccaaaaatgg taaacaaccc gatatatacc actgggttga gaaacaacga caacagatag
     1321 aacacaaaca gaattaggat gatcacaacg atgtacaacg gtttttccac cagatgattt
     1381 ttaatatgtc tcagatcata gagtatgtcg attttttctt ttcgttcctg accaatatag
     1441 cttggtcgta tccccaaccc aaaaaacaac aacacaaaca aaaatccgat gtggagagga
     1501 ttgaaaagat ctatgttggc aagaaaggtt aaccattcat ttgcgaatgt ggacatactt
     1561 ccccaaaaac caactaatga ttcaacaaag gtgtattcaa caggggttga gtatacaaac
     1621 cctttcccga tgataaaata actggcaacc agaacaagac ctgatggaat aaaaaaggga
     1681 ccaaagagga gaaacgagcg agctacccat tcaaagaccc cttttttttc tttctttggc
     1741 tcaacctttc catagttcac gctcacctgg aatgcagcaa tcccgcttct tgtgaccgta
     1801 tacccaagag cctgagaaat cgtttgaaac aaaagaccaa tgtaattagt aaatcgacag
     1861 aacgcctcat gacgatgccg taatcttgac aacatccgtt tttgaagttc aatgaagaac
     1921 atctgaagaa accagaacgc tgcgacacca aggattggcg cgagggctgc taccaataag
     1981 acatcaatat ttaatgggac gtttgccatc tcgctcagga agggtagtca tccgaggata
     2041 taaaaatctt ttttaaataa tctcaatgac gaccccacga cctgcataat cgtacgcctg
     2101 ccaggaaaca tccccataag cagacactcc cacgatgata tggattttac caaaatggcc
     2161 aaataaatcc aaatcagcat tagagggacg agctatggag gacggatggg aatgcaccgt
     2221 cccaacgata ctataatcaa caggaagcat atgcagttga aaaatcgcat gagaatcacc
     2281 agaaatcgta cctgggagga gtactacctc aatgatcgtg tttttttcaa cccggtcaac
     2341 tcgaagaagc gcgccgaatt cctgcggata attcgacttc gagctctcag aaatcagatc
     2401 caggcacttc ctggttatct tccaaggata tggtatcggc agtgttgttt ttcgtcgaaa
     2461 aagccccata acttatctca tgagttttaa acgcaccatc tggtaaaagc tttccccgaa
     2521 tcggatgaac cgtgccttct tctccgatcc tgtcacactg atcgtactct cctgggtgac
     2581 tggtgtttga aaccgtccat caatcaccag gttggtctcc ttgcttttcg gcagcaaatg
     2641 tactcctacc tttcgatcaa gggggacgat ccagggtcgt gcagagagtt taaaggcagc
     2701 aagcggagcg atgatcatcg cattcaccgt tggatccatg atagggccac cgacgctcaa
     2761 cgaataactc gtcgaaccgg tcggtgtcgc gataatgacg ccatctgccc ccattgattc
     2821 aatcagctca tcgtccacaa atagttttag gtagagtatc tttgctatcc gtgctgtttg
     2881 aatcgtcacc tcattggtcg catcaggcaa tcgtttccca tcaagggtga tttttagttt
     2941 cgcccgttcc tctacattat atttcccggc gatgacctgt tttaacccct ccattgcgta
     3001 ctttgattcc acctcggaaa ggaacccgat tcctcctgag ttgatcgcga atactggttt
     3061 ttctatctcg ctcatcgcca tgaggatcgt cccatcgccc ccgatggtca ccacgatatc
     3121 tgcgttcttg ttgatctctt tgaacgaaga tcctttgtat tggatctctg atgcgatcgc
     3181 ttcctccacc agtacggtcc catgctgttg taaaaactca gcgacatcct ttgctaatgt
     3241 cacggttcgt ttcacacccg ggttgcacac caagccccaa ttcgtggtca cgtttacgcc
     3301 ctcgccagtt tcatcagtgg tgtcagcttc accaacaatg atggatgttt catcaccaac
     3361 gccttaataa tactaagggt actgaactcc tcaaatttat aatccttcaa ggaatcagcc
     3421 agcatatcca atgttttatc cgtcatgtca agcatgattt cttttgctat gtagttccga
     3481 tgcagttttt tcccaaacgc gttcttccaa cgagtttcgt actcggagag catctccgaa
     3541 tcgaaacgct gtttctcgac tgcttttcca atggtttcac cagcgtatct tcccgcctcg
     3601 atcgaatgca tcagaccacc gccggtgata ggatcgactt gacgtgcagc gtcaccaact
     3661 aaaaggaggt tgtccgtaat ggattggatc ggttcagcga ccggatctgc tccggtgagg
     3721 atccgcagag gctttccttt ttttaacgct ggttgagcag cgatgaattt gtcaagatac
     3781 tgttttgctt ttccagcttc actgagactg gcaagaacac cgatgccgac atttgcaaca
     3841 tctttccctt tcgggaacac ccagacataa ccgccagggg cgacatgttt tcctaagaag
     3901 aaatcacagt acgggctgtc ccaatcgata ttggtcaagg tgtactggac gcaggtttcc
     3961 aggtcatgtg gtttcagagt ggttttaatc cccgcccatc tcccaactct tgactcaact
     4021 ccatcagcac caacgatgac atcagcctcg atatcgaggg tctcatcgaa ctgctgtgcc
     4081 gtaatacccg taatttttcc attttttttc agcagatcaa ctgcaagggt attaagcata
     4141 agttccgcgc cttttttcac tgcacctcgt gctaactcct gatcaaagat atctcgatac
     4201 acgacgtacc ctgtttcatc tcccgcgtac tccgcagcta acgttacatg agtcccattc
     4261 ggagaaaaga ttcttgcccc cttcattttt gtcgcaatcc accgttttcc ctcaagaacc
     4321 ttgaaattgt cgaccttctg actaatcccc tcgccgcaga ggacaggaac accgacttca
     4381 gcgcgacgtt cgatgatgag aacctttgct cctgcttcag cggcaaaccg ggcagtcaca
     4441 ctcccagcag gcccacttcc gacaacaacc gcatcatagt gcatcttatg ttccctccca
     4501 ggttatcgcc ccgattgggc aggtctttac acaaatcgaa cagctgttac acagttcatt
     4561 gtcaacgact gctttactgg aaatcatgag gatcgcatcc tgtgggcaga ctgagataca
     4621 gccgccacat gccaaacatt tctctttttt cacaactgcc ttatgctttg ccataggatt
     4681 cacctctggt gataccggac gctatcagat gtgctactcg gattggctca ggtatgacac
     4741 cacgtatggt agagatattt attatttctt ttgcctcctc taaagatatc ccagtgcatt
     4801 tgatatatat cgggttatgt tgcgtgggta tcctatgcaa tgatcctttc tgaaggaggt
     4861 tccatcgctg ctgccagtcc ttgaaatttt tttgtaacgc ctgttttatt tttctctgat
     4921 ctggcttatc tcttgtgatc gtgatcaccg gcagatgtgt cgattcagaa agatcatcga
     4981 tatcaacgac gttgaaccca ccgaggcagg cgccgtctat catcaccgtc ttgagttgtc
     5041 gtcgatatct tgagcgttgg atcatctgct tacagatttc agtcgcttct gttccatcaa
     5101 tggtcacttg gtttcgtaag acaccctcga tatattcacc tccgcgcatc actacaccga
     5161 tgatggttga atatttttcc gtgaatgtaa acggcgcatc gtctatcccg aggattcgga
     5221 tctgtcgctt catctgagca ataaaacccc tgggtcgatt aagaatgttt cgatacgcat
     5281 ccttggatca cgatacactt ttgttccgcc acctaatgtt acatattata tattataggg
     5341 gggctgctcc atccaccaca caaatccgtc gcatgaagaa ataaatgatc aaggacctag
     5401 gtcgcatgat aaaaagacat tgattagaaa atgacgcacg atcgtcttct tatggaaaaa
     5461 tggaatcgac tgtttcgtat gatattttta tactgacaac tgatgatttt tttttaaaac
     5521 gtcaaaccgg tcagcgtctt tgtgtcaagc acgccgttga tcgctcgtat tttattgacg
     5581 atgatggtgc cgagttcctc gaaatcattc gcatcgattt ttgcaatcaa gtcgtactcc
     5641 ccaaacaaag ggtgtagctc gacgatttga ggtatttttg agagtttatt gtagacttca
     5701 tgttcatgtg ccggggcgac atttatgaga acaaaaccta tagccatcta gtatcctcct
     5761 ttttttttta ttttgttttc aacattgtac atcattctat ttctattctt ttttttttac
     5821 agaacctaag gttctacttc gtcagtgctt acctgtccgc ttgctgcaag cttacagctt
     5881 tcgcagatgc cttccatttc ttcctcaggt tcgagttcgt ctccgcaaac agagcaatac
     5941 agtgacatag tataccatcc ctctcactgt gtcagtgacg agtatcacat cgcaataaga
     6001 ttattgttta taaagtcttt tctccacttc tcaaacctct gtcatttatc aaattttttt
     6061 ttatcttcat tttagatcgc ttcatacgca tcaaaaattc ctatgaaaac acgaactact
     6121 ttttttgttt tgaacacctc atcatttttt tttaaaaaaa aatctatcgc aaaatcatcg
     6181 acagttacaa taaattttaa tacatgtttt ttaatatggg gtacgtagtg agtcttttat
     6241 aatgaaaggg agaaccacat ggatttgacg ggccttcaaa ctgaagatga gcccccacct
     6301 ctcaatggcg ttggagatac tcttttttct cgagccagga atcttgagcg tgtgcttggt
     6361 tttggaaaca tcttcttaaa attcgagggt ggcaacccga ctgggacgca gaaagacaga
     6421 atctctcagt atcacgtaaa aaaagcaatg caacaagggt acgataccat caccgtggga
     6481 acctgcggga actacggagt gtccctggcg tattttgcga aactcgcggg gttgaaagca
     6541 aaaattctga ttcctgaaca gtactatgtc gcgccaaaac gaaaagaaga actgcaaagc
     6601 atgaatgcag agatactaac gatcgatggg aaatacgagg atgcagtcga agaaagtaga
     6661 cgactatcgc gcatggagca tgtgtacgac gcgaaccctg gaaatgggaa cgagggatgg
     6721 cgcggataca tgtcgatcgc atacgaaatc tatcggcagc tcggccgcac accaaccgca
     6781 gtcgccgcac cggtcggcaa cggaacaaca cttgttggga tctaccacgg gttcaagaat
     6841 ctgcttgacc ataacatcat cgaccacatg ccgtaccttg tcggcgcatc aacacccggg
     6901 ggaaacccca tcatcaaaag cttcaaaact ggtgcgtcaa aaacccagga cctcccgcct
     6961 tcggaaatca aagaaagcag catcaacgaa ccactcatcg cctaccattc ctacgacggt
     7021 gatgacgcac tgcaagcgat ttatcagacc aacggctggg ctgattacgc gtctgattcc
     7081 tgcatgatga aattccatac catgctgcga gatttggaag gactcaacgt gcttcccgca
     7141 tcagcaagtc cgattgaagc actgataaaa ttcaaggaac atcgagtcct gaacagtgat
     7201 tatgtgattg ttcttacggg aagaaaattt cggtaacgat tctaccacgg attgattcct
     7261 ttatggcgaa gatgcgcgcg ttggtctatg cagaccgctt gtacaattgt gctgatggaa
     7321 aaaccgctca cgggcttgta cggttcagta aaaagttcga catcgtctcc gtggtcgact
     7381 ccacgctacc catcggtgat gccgctgaat ttttagatgg aaaaaagaaa ggaatacctt
     7441 tgttgaatga ccttgatgat gcgttgtcat cgacacatcc agacacgttc atcatcggtg
     7501 cggtatccga aggcggtgtg ctgcccaagg gatatgataa agcggtggac tgggcattat
     7561 caaaagaact gaatgtcgtc tccggtcttc atcaatttct ttcagacgat ccacgattca
     7621 tcaaatgtgc agcagaacat cactgcacca tcacagatgt tcggaagatc ttccgtgacc
     7681 acaaacgatt ctacaccggt gaaataacac aggtaccttc gattcgcatc gccctcatcg
     7741 gaacagattc cgcgattgga aaaagaaccc ttgccgtctt gctcgtcaac gaattacaga
     7801 aacgcggaag aaccgcagac atgatcttca ccggacagac cggctggctg caaggctggc
     7861 cacatggcgt ggtcttagat gcgatgatca acgatttcgt ctcaggggga atcgaaggag
     7921 cgatcattga ctcatggaac gatctccacc cggagttcat gatcatcgaa ggacaaggca
     7981 gcctggtcca tcctttcttc ccaggtggct ttgaaatttt agcagctggt cagatacaag
     8041 ggttcatctt ggtcgatgca ccaaaacgac cacaccttga tggcttccca gactatccca
     8101 tgccagaccc aaaacgagtg gtaagaattg ctgaaatgct caccgaaaaa agactcatcg
     8161 gcgttggcat aaaccatgag aagatgaaca catccgatat cactcgtgca aaaactatgt
     8221 atcagaagat gttcaaagtc cctgtatcag aaccactttc agaaggagtc ggtgttctag
     8281 ccgatgcgtt ggaggttctg tcccatggca aaaaataatc ttctgtgtct ttcgagcatt
     8341 tctctttcag atccgatgat tgagaaaaat gtagctcgag cagagatcat cgtcattgaa
     8401 tctgacggga cagaaagtcg tttcgaactc atgaacaaat atgagcatga gctaatgcaa
     8461 tctcatctac cattactgcg actcgcgttc tgcatgccgt tgctcaacta cggactcttt
     8521 tcaaaaaaaa taaagttgaa tttcccaata agcaaggcag atatttcgtt gctcaatgac
     8581 ctgaatgtca tctttagtcg tgatatcttc gtcaacaaga ttgcacaaaa aaccaacccg
     8641 tacatcctcc cagaattttt ccctgatcaa gagaaaatag gaccacatga tggtgatcct
     8701 acggcggtca tacaaccaaa acaaatcatt gacgatgtgc caatcagtga gaatatggat
     8761 gccatgaaat caggagtcct ttccagcgga gggaaagaca gcctgctgac gtacgggtta
     8821 ctcaaagaac tgggttcaac agtgtatccg ttgtatatga acgagagcgg tggtcattgg
     8881 aggactgcgc tgaccgcgta tcagaatcat aaaaaaactg agcctaacac gcagagggtg
     8941 tggacgaacg tcgacaggtt ctatagtttc atgcttgatc atttacggtt catacgatcg
     9001 gatcatcgaa agatctggca tgatacctac ccgatacggt tgtgtatttt cccgttctat
     9061 gtgttcagct tactgccgat ttttgttgaa gaacgaatcg ggaatttatt gttaggcagt
     9121 gagtttgacg atctgcgcta cgaggtccag tacaagggaa tcaaacatta cttcggagtc
     9181 tatgatcaac accaggatta tgatgtgagg atgaatcaat ggtacgagaa acgaatccct
     9241 ggtctgtatc aatggtctgc gttgcggaac atctccggtc ttgtcgacca gaacatctta
     9301 gttcgtcgat atccagcact tgcaaagttg cagcgatcct gccattcctg tcatatcaaa
     9361 aacggtgtgg tgtacccatg cggaacgtgt tcgaaatgtc taggtattct gctgtacctt
     9421 ttggctaatg acctcgatcc gaccctcatg aagtatcggc gcaaagacat cgaagatttc
     9481 tatataaacg taggagtatc atccctgaaa cttgataaag atgagaaaga ccaatcgttt
     9541 tacttgctca aacgccaagg gtctataccg aaggtgcagt gcgtggatca tgtcgagaaa
     9601 attcatgtcc atgaatcgac atgtgaccct aatctctatc ccagacaatt caggaatggt
     9661 ttactcaaaa tccttgagga atatacgaca ggttattgtt cattaaaaaa taagaaatgg
     9721 gtccctacga acaaatagca agcagttact ccagttcctt aaccattcgg taagcatcac
     9781 gggagccttc atgatcatag ttgtaatatt tttttagcag catggttttt tgaaaaccag
     9841 cgtgttcgta gagcattatt gcagcttgat tgatgatagc gacctccagt ctgattttat
     9901 gcattccttt ttttctgata cgttcttccg ctgcagtaag caacctttgg ccgattcctt
     9961 ttttccggta cattggatcg acgttgatgg tttcgactcc tgcgaccttg gttcctctcc
    10021 gatataagat aatgatgaat cctctaagga ctgtactggt tttttcgaca agtacagtgc
    10081 tgttggcttt catgaggaga tggcggagtt gtcgtcgtgt ataggcgagt tctttgggaa
    10141 aacagatctt ctcaagtgcg acgattcttt cgaaatcgtc aggagaagct gttctgatgt
    10201 ggatggtttg ctgcattgtg gtagggtaca tgaatc
//
LOCUS       DATMXJ010000177         6946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_28956, whole genome shotgun sequence.
ACCESSION   DATMXJ010000177 DATMXJ010000000
VERSION     DATMXJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28956"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VN377_04580"
     CDS             complement(<1..809)
                     /locus_tag="VN377_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWR27696.1"
                     /translation="MAKKQNKTDNERKEKKDSLKEIKIAYRSLDDRKKFIRSIFIPLI
                     FLGVLVMCLPFLLQLLITSQLQYNPITFIFAGTIPIILGVFYPYISWKNRESDINGKM
                     HFFITHIRVLAISDLSLKDIVNVIGGNKAYKSLGEELRKISVLADQWRVPISKTFRFV
                     GERTPSKILRDFLDRFSQSLDSGVEHREFIEHEQDAVLQEYKTIYEASNENVVILSEV
                     YVSLLIAIIFVMSMGIVLPMVMGSQDMGSFIYVSSFMLLASEVMLLYLLRS"
     gene            complement(816..2465)
                     /locus_tag="VN377_04585"
     CDS             complement(816..2465)
                     /locus_tag="VN377_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWR27697.1"
                     /translation="MDEMQKEEVAQLLAENQHLKEYLESIKQKMANPIFYSKIPREIR
                     NESYPNFIYPTKGVVFIHIYRTQDMDELEYHVIEPTVNDVLKEKLDIILKLIVKKAPE
                     KKSVITDDELREVLKELIDEITVINERSEKLDVSKKKGKVSTPEKVKVTSYQKSVLQY
                     YIIKTLVGGGPLEAFMRDPYLEDIHFVANEKIHLIHKVFGMIKTNVVVSEKEAPVFAK
                     AISEKMGAPVSEGRPIVDGVLPDGSRGNIIYSSAVSIKGPSMTIRRFTETPISVTQLI
                     KWGTLSSSIAAYLWLCLQYGRSFFICGGTACGKTTTMNSIIPFIPPQKKIYTAEGTPE
                     LQVPHTVWQRLLTKTTGPKEGQVDLFDLLKAALRSRPDYIIPGEVRGAEGNIVFQAMQ
                     TGHPCMTTFHAGSITQVIQRFTGDPINVPKTFMDNLDFVVIQLAIERNGKMIRRCTAI
                     DEIEGYNREVDGIMSRTAFTWKADEDVHDFKANRNSFILEEKIAKNAGYSDTAKIYDE
                     HERRKHILDRLVEEDIMDYYEVVQCIWGFYREGEKGIPISV"
     gene            complement(2539..4167)
                     /locus_tag="VN377_04590"
     CDS             complement(2539..4167)
                     /locus_tag="VN377_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HWR27698.1"
                     /translation="MTKMEKKEEEKIIKENPHLQTYLDELRKKIERPIFYSKLPRDLK
                     EEKYPNIIYPTKGSVFIHIFRTKDMEGKEYHAIEPSLNEKGKIKRDQILDLIYEKAPF
                     WKAVKTDDEIKEAIRTLLDKLTVIDERSTGNVKGSGGKLRVTSEEKKSIEYDITKNII
                     GGGPLEPFMRDPYLEDIHIITGENVHLIHKVFEMIKTNIFIDKAWANTFSQEFSEKIG
                     SPVSEGQPIADGTLPDGSRVNIIHSKDVSLKGPTMTIRKFSETPISVTQLMNWGTFDA
                     GVAAYLWLCLQYGRSLFVCGETASGKTTTANAIIPFIPPEKKIFSVENTPEVQVPHAV
                     WQQLLTKSTGPKEGHIELEDLLRAGLRSRPDYIIPGETRGIEGRVVFQAMQTGHPVIT
                     TFHAGSVTKVIQRFTGHPINIPKPFMDNLDVVLIQMAVERKGKRIRRVLSVDEVEGYN
                     KEVDGIMSRKAFEWNAVDDTHSFKANRNSYILESRIAKMAGLTDPMKIYEEFDRRKHI
                     LERMLEEKIFDYYEVVQFIWTFYREGEKALPISI"
     gene            complement(4180..4893)
                     /locus_tag="VN377_04595"
     CDS             complement(4180..4893)
                     /locus_tag="VN377_04595"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWR27699.1"
                     /translation="MEGVKSIILERKGEKDGFAERFGKVIPNGSLIFIEGKEGTGKSI
                     FCQRFCYSLLKNNCSCSYVSTQFTIKSFLRQTASVGYDVRKFLMAGKLLFISTEVTLA
                     ETLPRKTFLEGLITGRKLFDPEIIFIDSLSTLLKDSVNKENLTDLTSFFNRLTGSGKI
                     IFITANPNEWPEEIHNTFRMITTIHFRTSRETAPGIGIVYNMYLEKFNGARYKYDPVT
                     RFSVRPGVGLTIESSGVAF"
     gene            complement(4915..5355)
                     /locus_tag="VN377_04600"
     CDS             complement(4915..5355)
                     /locus_tag="VN377_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27700.1"
                     /translation="MGFSLTGTHVIFFVAAVIVAGMVSGVFIAVTMNVSTSLSNRGDR
                     VSEKLNTDFDIINDPNIIPMTGLYYIFYLKNIGGVRLTTSNQTFQVFIDGELIGITQY
                     YFENSSIPIDGVTSLYVNTTITGGDHTLRIVGPQAVDKEFAFMK"
     gene            complement(5367..5792)
                     /locus_tag="VN377_04605"
     CDS             complement(5367..5792)
                     /locus_tag="VN377_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27701.1"
                     /translation="MGFSVIAAAAILGLTLFMAVEIIASDLLPTIEDMNKSYDELKER
                     HQNQLQSDINITMVLRSANGSNYDYNISVKNTGSATLFTNDFEILINGSEYQFTCPRE
                     NLYPENTVYFHIKNVAGAGTQRLKVITNNGIADYYTYTI"
     gene            complement(5897..6703)
                     /locus_tag="VN377_04610"
     CDS             complement(5897..6703)
                     /locus_tag="VN377_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0097588 - archaeal or bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlaD/FlaE family flagellar protein"
                     /protein_id="HWR27702.1"
                     /translation="MSEGDQNQGEKILEDNEVGSELSILAEQNILPQKIALRIAEKLK
                     EKNIRITKNQLYRLTDKIQSILINYKASVPNQPEQPKKVETELLNNTIIQTTDMKRLV
                     EAVEQLNQRIKVIEEHRIDGVKGASGTLVKTKDIKTFESTDILEGNIQPLEHIPSDPE
                     SIVVTMKWLQYLVDKTGKSNLPDTLGYYVDIGWITEDVRLDLINYSKGITEEPAQTDT
                     RPPQLPTKDHLQSLLFIQKLKGLQLDDRFLNKIERDMEKIVKSLEGYPLK"
     gene            6766..>6946
                     /locus_tag="VN377_04615"
     CDS             6766..>6946
                     /locus_tag="VN377_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27703.1"
                     /translation="MVSIFCSIRSDSNDLFRRSSIWFSFHGSKAVVCNDVGRDGEMTS
                     GALGIGEASDFTFGSM"
BASE COUNT         1701 a         1511 c         1401 g         2333 t
ORIGIN      
        1 atagaacgca acagatacag cagcattact tctgaagcga gcaacatgaa cgatgaaaca
       61 taaatgaagc tccccatgtc ctgtgatccc atcaccattg gcaggacaat ccccattgac
      121 atcacaaaga taatggcaat aagaaggctg acgtacacct cacttaatat aacgacattt
      181 tcattgcttg cttcgtaaat cgtcttgtac tcctgtagga ccgcgtcttg ttcatgttca
      241 atgaactctc gatgctctac cccactgtca agactctggg agaaacggtc aagaaagtct
      301 cggaggatct tacttggtgt cctttcaccg acgaatcgga acgtctttga tatagggaca
      361 cgccattgat cagcaagcac gcttatctta cggagctcct ccccaaggct tttatacgct
      421 ttatttccac caatgacatt gacgatatcc tttaagctca gatctgaaat cgctaacacc
      481 cgtatatgag tgatgaaaaa atgcattttc ccgttgatgt cagattccct gttcttccag
      541 ctgatatatg ggtagaaaac ccctagaata attggtattg tcccagcgaa aataaacgtt
      601 atgggattgt actgtaactg acttgttatg agcagttgga ggagaaacgg taaacacatc
      661 accaatacgc caaggaagat caagggtatg aagatggatc tgatgaattt cttgcggtca
      721 tcaagactgc gatacgcgat tttaatttct tttaatgaat cttttttctc ttttcgttca
      781 ttgtctgttt tgttttgctt ttttgccatt cttcctcaca cagagatcgg tatccctttc
      841 tcgccttccc tataaaaccc ccagatacac tggacgacct cataataatc catgatgtct
      901 tcttcgacca aacgatccag gatgtgtttg cgtcgctcat gctcgtcata gatcttcgca
      961 gtatcagaat accctgcgtt tttcgctatc ttctcttcga ggataaagct attcctattt
     1021 gctttaaagt catggacgtc ctcgtcagct ttccaggtaa aagcggtacg ggacatgatt
     1081 ccgtcgacct ccctgttgta tccttcgatt tcatcaatcg cagtgcatct ccgaatcatc
     1141 tttccattcc gttctatcgc aagttgtatc actacgaaat caaggttgtc catgaacgtt
     1201 tttgggacat taatcggatc cccggtaaac cgctggataa cctgagtaat actccctgca
     1261 tgaaacgtgg tcatgcatgg atgccctgtt tgcatcgcct ggaacacgat gttcccttca
     1321 gcgcctctta cctctccagg gatgatatag tcagggcgtg accgtaaagc agcctttaaa
     1381 aggtcaaaga ggtctacctg accttctttt ggtccagttg tcttggtcaa gagtcgttgc
     1441 cagacagtgt gcggcacctg aagctcagga gtcccttcag cggtgtagat ctttttttga
     1501 ggaggaatga atggaataat cgaattcatg gtggttgttt ttccacaggc ggtcccaccg
     1561 cagatgaaga aactacgtcc gtactgcagg cataaccaga ggtacgccgc gatacttgag
     1621 ctaagagtgc cccatttgat taactgtgtg acgctgatgg gtgtttcagt gaaccgtcgg
     1681 atcgtcatgc ttggtccttt gatgcttacc gctgagctgt agatgatgtt ccctctgctt
     1741 ccatctggta ggacaccatc gacaatcggt cgaccttcac tgactggtgc acccattttt
     1801 tcactaatcg ccttggcaaa cactggtgct tctttctcgc tgaccaccac atttgttttg
     1861 atcattccaa atactttatg gatcaggtgg atcttctcgt tagcgacgaa atggatatcc
     1921 tcgagatagg ggtccctcat gaacgcctca agaggaccac cacccacaag tgttttgatg
     1981 atatagtatt gtagcacgct cttttgatag gaggtgacct ttaccttctc aggtgtcgat
     2041 acttttcctt tctttttgga aacgtcaagt ttttctgatc tctcattaat cacggtgatt
     2101 tcatcgataa gctcttttaa cacctctcgt aactcatcat cagtaatgac actttttttt
     2161 tcaggggctt tctttacgat caatttaagg atgatatcga gtttttcttt tagcacatcg
     2221 tttactgttg gttcgatgac atggtattcg agctcatcca tatcctgtgt tcggtagata
     2281 tggataaaga caaccccttt ggttgggtag ataaaattag ggtatgattc atttcttatc
     2341 tcccgaggga ttttactata aaaaatgggg tttgccatct tttgttttat ggactctaga
     2401 tactctttta ggtgttgatt ttcagcaaga agttgtgcca cttcttcttt ttgcatttca
     2461 tccatgtttt gttcacctta gttctctgct aatttctttc attccgtccc cattctctgc
     2521 ctttttttat caaatatttt agattgatat cggtaatgct ttttctcctt ctctgtaaaa
     2581 cgtccaaatg aattggacga cttcataata atcaaagatt ttctcctcaa gcatgcgttc
     2641 gaggatgtgt ttcctccgat caaattcttc atagattttc atcgggtcgg ttaatccagc
     2701 catttttgcg attcttgatt ctaatatgta gctgtttctg ttcgctttga acgaatgggt
     2761 gtcatctact gcattccatt caaaggcttt ccgcgacata atcccatcaa cttctttgtt
     2821 gtatccttct acctcatcga cagataacac ccgtcgaatg cgttttcctt ttcgttcaac
     2881 cgccatctgg attagcacca cgtccaggtt gtccatgaat ggttttggaa tgttaatcgg
     2941 atgaccagta aaccgctgga tgaccttcgt aacacttcct gcatgaaacg tggtaatcac
     3001 tggatgaccg gtttgcatcg cttggaacac aacacgtccc tcgatccctc tggtttctcc
     3061 tggaatgata taatctggtc tagatcgaag accagcacga aggagatctt ctagttcgat
     3121 atgtccttct ttaggtcctg tggatttcgt aagcaattgc tgccatactg catgaggaac
     3181 ttggacttca ggggtgtttt caacagagaa aatcttcttc tccgggggga taaatgggat
     3241 aatcgcattc gcagtcgtgg ttttaccaga agcggtttca ccacacacaa acagactccg
     3301 cccgtactga agacataacc agagatacgc tgcgacacct gcatcaaacg ttccccagtt
     3361 catcagctgg gtgacactga tgggtgtctc actgaatttt cgaatggtca tggttggccc
     3421 tttcagactc acatctttgc tatggatgat attgacccgg cttccatcag ggagtgtccc
     3481 atcggcgatt ggttgacctt cgctgactgg gctaccgatt ttctcgctga attcttgaga
     3541 aaacgtattc gcccaggctt tatcgatgaa aatgtttgtt tttatcatct cgaagacttt
     3601 atggatgaga tggacatttt cccctgtgat aatgtggatg tcctcaagat aggggtctcg
     3661 catgaacggc tcaagcggac caccgccgat gatatttttt gtgatatcgt attcgatact
     3721 ctttttttct tcggaggtca cacgaagttt ccctccactc cctttcacat ttccggttga
     3781 tcgctcgtca ataactgtaa gcttgtccag taaagttcgg attgcttctt tgatttcatc
     3841 gtcggttttt actgccttcc agaatggcgc tttctcatag atcaaatcaa gtatttggtc
     3901 gcgttttatt tttcctttct cgtttagact tggttctatc gcatgatatt cctttccttc
     3961 catatccttg gtccggaata tatgaatgaa gactgatcct ttcgtcggat agatgatgtt
     4021 tgggtatttt tcctccttta aatcgcgtgg taatttgcta taaaaaatcg gtctttctat
     4081 tttttttcgt aactcgtcga ggtacgtctg gaggtgaggg ttctctttga ttattttttc
     4141 ttcctctttt ttctccatct ttgtcatacc catcactcct taaaacgcga caccacttga
     4201 ttcaatggtc agtcctactc cgggtcggac cgaaaaccga gtgaccgggt cgtacttata
     4261 tcgagcgccg ttaaatttct caagatacat gttatagaca attccgatgc ctggtgcggt
     4321 ctcccgagac gtccggaaat gaatagtggt aatcatccgg aaagtgttat gaatctcttc
     4381 aggccattca ttgggattcg cagtaatgaa gataattttt cctgaccctg ttaatcgatt
     4441 gaaaaaactg gtcaggtcag ttaagttttc tttgtttaca ctatctttta acagagtgga
     4501 gagcgaatcg atgaaaatga tttcaggatc gaaaagtttt ctccctgtga ttaacccctc
     4561 aaggaatgtc ttccgaggaa gggtttcagc aagggttacc tcagttgaga tgaataataa
     4621 tttcccagcc atgagaaatt ttcgaacatc ataaccaact gatgccgttt gtcgtaagaa
     4681 actcttgatg gtgaattgtg tcgatacata cgaacaagaa caattatttt tcaagagact
     4741 gtaacaaaac cgttggcaga aaatactttt gccggttcct tcttttcctt cgataaagat
     4801 taaggaacca ttggggatta ctttcccgaa tcgttcggca aatccatctt tttccccttt
     4861 tcgttctaga ataatggatt ttactccttc catagttcct catacctttt ttttttattt
     4921 cataaaagca aattctttat caacagcttg cggtcctaca atccgtagtg tatggtcacc
     4981 acctgtaatg gtggtgttca cataaaggga tgttactcca tcgataggaa tcgatgaatt
     5041 ctcaaaataa tattgggtaa ttccgatgag ttccccgtcg ataaagacct ggaacgtctg
     5101 atttgaagta gtcagtctca caccaccgat atttttcaga taaaagatgt aatataaccc
     5161 tgtcatcggg atgatatttg gatcgttgat gatatcaaag tcagtgttga gtttttcaga
     5221 tactcggtct ccacgatttg aaagacttgt ggagacgttc atggtgaccg ctataaacac
     5281 accagacacc atcccagcga cgatgactgc tgcgacgaag aaaatcacat gcgtcccggt
     5341 tagactaaat cccatctatt tttccctcat attgtatagg tatagtaatc agctatacca
     5401 ttatttgtga ttactttcaa tcgttgcgtc ccagcacctg ccacattctt tatatggaaa
     5461 tatacggtgt tttctggata gaggttctca cgaggacagg tgaattgata ctcggatccg
     5521 ttaatcaaaa tttcaaaatc atttgtaaag agggtggcac ttccagtatt tttcactgag
     5581 atgttgtaat catagttcga cccattagct gaacgtagga ccatggtaat gtttatatcg
     5641 gattggagtt gattctgatg tctctctttc aactcatcat aggacttgtt catatcctcg
     5701 atggttggaa gaagatcgct tgctatgatc tcaacagcca tgaataacgt caatcctaag
     5761 atagcagcag ctgctatcac tgagaacccc atcttttata ccctctaagt gagttctatg
     5821 gatgtgcatc catcgaatca aataaccgat atgtaatatg atatactgat aataaagttt
     5881 cttttttgtc ggcttgttat tttagtggat acccctcaag gcttttgact attttttcca
     5941 tatctcgctc tattttattg aggaaccggt cgtccagctg taatcctttg agtttctgga
     6001 tgaacagtaa tgattggaga tgatcttttg tgggtaactg tggtggacgg gtgtctgttt
     6061 gtgctggttc ttctgttatc ccctttgaat aatttatcaa atctaatcga acatcttcag
     6121 taatccagcc gatatccaca taatatccca gggtgtcggg aagattgctt ttccctgttt
     6181 tgtcaacaag gtactgtaac catttcatgg taacaacaat gctttccggg tcactgggta
     6241 tatgttccaa tggttgaatg ttaccttcaa gaatatctgt tgattcgaag gtttttatgt
     6301 ccttagtttt cacaagtgtg ccacttgccc ctttcactcc atcgattcga tgttcttcga
     6361 ttaccttaat gcgttggttc aattgctcca ccgcttcaac tagccttttc atatcggtgg
     6421 tttgaatgat ggtgttattc agtaattctg tttccacttt ctttggttgt tctggttggt
     6481 ttggtacaga tgctttgtaa ttgattaaga tgctttgaat tttatccgtt aaccgataga
     6541 gttgattctt cgtaattctg atgttttttt cttttagttt ttcggcgatt cgtagtgcga
     6601 ttttttgagg tagaatattt tgttcggcta ggattgataa ttcactacca acctcattgt
     6661 cttcaagaat tttctctcct tggttttgat ctccttcgct cattcacctg ctcctttcta
     6721 tgaacagagt tcctttattt taaatttatg aaaaattcat taatcatggt atcgatattt
     6781 tgttccatca gaagtgattc taatgattta tttaggaggt catctatttg gttctcgttc
     6841 catggttcta aagctgttgt atgtaacgat gtcggtcgtg atggtgagat gaccagtggt
     6901 gccttgggta taggagaagc ttctgatttt acgtttggct caatgg
//
LOCUS       DATMXJ010000178        12101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_34921, whole genome shotgun sequence.
ACCESSION   DATMXJ010000178 DATMXJ010000000
VERSION     DATMXJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34921"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..324
                     /locus_tag="VN377_04620"
     CDS             91..324
                     /locus_tag="VN377_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27704.1"
                     /translation="METYSWLDTTNGKELSLFAVELKEKFSTLTVGESSSMLGSAMAA
                     SMRRKAKELDISPDEAVDYYFYTVMNKNIRTKK"
     gene            complement(424..741)
                     /locus_tag="VN377_04625"
     CDS             complement(424..741)
                     /locus_tag="VN377_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27705.1"
                     /translation="MKYYVYVIRLSDAVLKSRKFRRRNPDMIPGFPCFYVGQSCHFPE
                     TRFWQHKKGYKGNRFVKEFGLGLCPRLYERFNPIGTRKEAEIIEAKLTESLRIKGHGV
                     WSN"
     gene            complement(823..3138)
                     /locus_tag="VN377_04630"
     CDS             complement(823..3138)
                     /locus_tag="VN377_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWR27706.1"
                     /translation="MNSSRKITGIFVCLLMITVAYPSIGAGSPFGGLFDDLTPHHTLV
                     RVTTGSGVPVLPKNIEIASDNPGAWVDVIIPSDRLHEFSEMNVPYSIIIEDLEAYDTM
                     MAGSYHTLAQIQTILQGIAANYSDIATLYSIGTTYEGRSIWCLEISDHPGVDEGEPGV
                     FYMGVHHAREWPGAEICLHIANNLTSLYGVDSTITDLVDNRRIWIVPVTNPDGYYYCH
                     DQGNDWRKNRDYFPQYGSYGVDTNRNYNGSCDGNILGSWGSIPSWANSHDSNNECYGG
                     PGPTSEAENQAVASIFLENNISATITYHTYGETVMWPWGYTGSQTPDNTYLSSVGQQI
                     AQRINIQSGPGTYDPHQSYGLYATVGDTIDYAYGYSHFVQGRATFAYCIETCNEFHPA
                     SSYLNQICKENCDGAIYLLQEAQNIRDTVVPRVIPPVISTMQNDTDGNYRVSWTLQNP
                     NANPDYFQLSEMKNLSLATDDAESGSAFWKLEGFTLSPSRYHSGAQSFKSRTGEYDVS
                     SMTTRTPVPITTGMTLSFWCWYAIQQNYDYAYVEVSENGRNYHLLDSLTGSSGNWIHK
                     EYSVDNFTGKSLFIRFRYATDGYGHQEGFYVDDISPMGIFGSTSILSSTITNTYYDII
                     DKPLGTYYYSVRGHNTVRGWCDNSTLRIINVTHGGGSTNPALILGNFTASPGKISMTV
                     KNIGDANATSVSVTLRVTGGLFGRINAETTKILRIGIGEEKTVTTDGFIVGLGKITLS
                     ASANCIEAVPPQVTKNATGKILLFFIYGIQN"
     gene            3364..4095
                     /locus_tag="VN377_04635"
     CDS             3364..4095
                     /locus_tag="VN377_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27707.1"
                     /translation="MNRDEASHGIQTALELVLPFSFGFIIALLFLRELNPSVEKLHWF
                     FGSSIGALVSLLGLIGLVITFRIQHIREQIRDVQSAILSYSKDGLCWVFAGFDFDEIT
                     RRMDWLFSQAFKTHEEDLKSDKNPEDIEYDQANLDAYRNERIRFGEMLKTYLDLENER
                     KGMYHMMVPPIVLTVGTTIVSIFGLISADIISSEGIYGSAFFVGAAALLIWLVVRVGI
                     FLITMMFKERKIRVYVTVDVADEKK"
     gene            4224..4676
                     /locus_tag="VN377_04640"
     CDS             4224..4676
                     /locus_tag="VN377_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HWR27708.1"
                     /translation="MNISIIYESKYGNGKEAMTYLENLLSKKGYSVQLMSVHEVKPQS
                     PPESDLYIFSAPNAFGKIVRSMRKFLEKTEIKNSSAQYILVNTCLNPSSSQDKGLEQM
                     EEILRTKNISKLSEGLKLKVMAIKGPLEAGYEKKLDELAQTIQSSLKK"
     gene            complement(4692..5165)
                     /locus_tag="VN377_04645"
     CDS             complement(4692..5165)
                     /locus_tag="VN377_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27709.1"
                     /translation="MTSIFLIVILCIYSIAPEDKKILGHIGLSFAIIYATMISVNYFI
                     QLTFVQFGAFNADVFSIKNPQSMMMTIEVLGYFFMGLSTLFIAPIFQSGKLEKAIRLL
                     FVFNGVLGIGGAIGFALNLDWRILIGGLLVWTIVMPITTALLAYWFKRGKSSAPR"
     gene            5363..5827
                     /locus_tag="VN377_04650"
     CDS             5363..5827
                     /locus_tag="VN377_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27710.1"
                     /translation="MPKNEDVYFSYFEHNKTIAHVLSLFCGFLFTSITILLTALKNPS
                     ETIAQATLLFLTILFYFSLFVLIDNLEMPFHYIKNIPPMTLKIRPFFYLLVIFYLFGA
                     TTVMMFLLFHLVYLSLISGMIWAIIVLLNIFSTAKRFYEQAKKRNWESNIPK"
     gene            complement(6066..7247)
                     /locus_tag="VN377_04655"
     CDS             complement(6066..7247)
                     /locus_tag="VN377_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWR27711.1"
                     /translation="MREIKKDIRPRPESFLDLIRREGRGNLKIFLGFAPGVGKTYRML
                     QEAHQLKQKGVDVVVGIVDTHGRDETENLLEGLEQIPQRKINYKGHDLFDMDMDAVLM
                     RLPELALIDELAHTNAPGGHNAKRWQDVEECLAMGINVMTTMNVQHIDSLNDDIARTT
                     KVRVTETVPDRFLDTLADEIVDVDLPVDELLNRLEEGKIYPRDRIATALHNFFQLRKL
                     TSLRELSLREVAKSVGAKAAKRDATLSEDSRGEVLKERILVAISSNSKNACLLIRKGA
                     RLAGELNTDWDVVSVESSKDNERTRTIEESQRLNDNMDLARSLGAKAVRLKGEIVPHT
                     ILDYARENRIGKIVVGKTWHPWFKRLFKDDIMDHILRESGSIDVYVVTFENPKKFHLR
                     T"
     gene            complement(7324..9372)
                     /gene="kdpB"
                     /locus_tag="VN377_04660"
     CDS             complement(7324..9372)
                     /gene="kdpB"
                     /locus_tag="VN377_04660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWR27712.1"
                     /translation="MTQDQKEIRRAARTRPLLDKKIISFSLKQSILRLNPRIQLHNPV
                     MFVVEVGAILVSLVSLFNLSTGHVFLFPIQIAIWLWFTVIFANVAEAMAEGRGKAQAE
                     SLRQTKVDTQAKLINKNGEIRVVNATDLRKGDIVLVTAGDIIPADGTVDEGVASVDES
                     AITGESAPVIRESGGDKSGVTGGTRVLSDSIRVKVSVDPGESFMDRMVDLVESAERQK
                     TPNEIALTILILGLTLLYMVVVVTLVAFASYTETDVSTVVLISLLVCLIPTTIGALLS
                     AIGIAGMDRLLARNVVAKSGRAVEAAGDIDVILLDKTGTITLGNRQAVEFIPAPGVSN
                     AEIRDMCVLSSLSDFTPEGRSIVELTLAQGASQKEALSGVPIDFSADTRMSGIDLPDR
                     KIRKGAPEAVTKYIDSLGGSVPIEVNEAVIRISREGGTPLMVSDNERIVGVIHLKDIV
                     KPGIADRLRILKRVGIRTVMITGDNPITAKTIADEAGVDEFIAEAKPEDKLGYIRKEQ
                     AKGQLVAMIGDGTNDAPALAQADVGLAMNSGTDAAREAGNMVDLDSSPTKLIDVIETG
                     KQLLVTRGALTTFSIANDVSKYFAIIPAMFPELPIFHAMNIMRLHSPESAVLSAVIFN
                     AIIIILLIPLALKGVAYRPAGASSILRRNSLIYGVGGMIVPFIGIKLIDILITTIGLA
                     "
     gene            complement(9402..11777)
                     /gene="kdpA"
                     /locus_tag="VN377_04665"
     CDS             complement(9402..11777)
                     /gene="kdpA"
                     /locus_tag="VN377_04665"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWR27713.1"
                     /translation="MRNVLKKYIGPAILSTVIFAVITGLAYPAFITGVAQVAFPHQAN
                     GSIIVHNETIIGSELIGQNFTHDKYFHSRPSNAGAGYDAMFSGGSNLGPSNPLLRQRI
                     QTEINESRLGGETNKDGTIPIDAVTTSGSGLDPHITLANALDQISRVAKARNISEETL
                     RGVVAQYVEGRDLGVFGEPRINVLKLNLALDTGKTVPSANVFTFDHGNPQFFGIIQAV
                     VIFVLLIITSYFLGTFLYNIVTGRPTKITKRFERVESWFYRMLHIDSKEDMNWKTYAF
                     CVLAFSFISFLFTYAILRIQGSLPLNPQGLTSVKPDVAFNTAMSFGTNTNWQVYAGEQ
                     TMSYLSQMLALTFQNFMSAAVGIVVALALIRAITKRKKGKGLGNFWVDMTKIVLYVII
                     PISIVAALFFVSQGVPQTFNGPIHATTIEGAQQVIPVGPVASQEAIKEFGTNGGGFFN
                     ANSAHPFENPTPFSNVIQIFLLLLLPLSILVMFGRMARQLKQGVLLFIVVLIFLIAAV
                     TVTTFEEQMGNRSLNTLGVDQSPSGLQAGGNMEGKEVRFGTYGSTMFAIATTSTACGA
                     VNSMHDSYTPLGGMIPLVLILLGEVVPGGVGAGFFCLFMYVLITIFIAGLMVGRIPNY
                     LGKKIISFDIKMTVLILISIETTILVFAALSVVTPDGLSSITNPGPHGLSQILYAFGS
                     GVGNNGSAFAGLNAASFWFIIMMALAMFIGRFFIIVPMLAIAGSFAEKYIYPPTAGTL
                     PTDNATFGAFLIGVIIIVAGLSFLPILVLGPILEHLLLFGGKLFLCGGLFI"
BASE COUNT         3561 a         2724 c         2494 g         3322 t
ORIGIN      
        1 gtcgtggagt taccagattt aaaaggcaga gaaaaaatca agggagaaaa aattttcagc
       61 attgtgaagt ttgagggaga atagataaaa atggagacgt atagttggct agatacaaca
      121 aatggaaagg agctttcgtt atttgcagta gaactaaaag aaaaatttag cactcttact
      181 gttggagaat cttcttcaat gctagggagc gcaatggctg catctatgag gcgaaaggca
      241 aaagaacttg acatttcacc agatgaagca gttgattatt acttttacac agtgatgaat
      301 aaaaatataa gaacaaaaaa ataggatttt tatatttcca atattttact cgtacattga
      361 cgcttggttc ccaatgggag cataacattt ttaaatgacg ttattttatt ttacatatca
      421 atttcaatta gaccagacac catgaccctt aatccttaaa ctctcagtaa gctttgcttc
      481 aataatctct gcttctttac gtgtccctat cggattgaaa cgctcatata accgaggaca
      541 aagacccaaa ccaaattcct taacgaagcg gttacccttg tacccttttt tatgttgcca
      601 gaaacgagtc tcaggaaaat gacaactctg accaacataa aaacaaggaa aaccaggaat
      661 catatctgga ttacgacgcc taaacttacg tgatttcaaa accgcatcag atagcctgat
      721 gacataaaca taatacttca taaaccgatc cattataata tcagtgaata acttataatt
      781 tactaagaaa tgaaaaaaaa agttcaaatc ctttcccttg cgttaattct ggatcccgta
      841 gatgaagaac agcaggattt tccctgtcgc gtttttcgtc acttgcgggg gaaccgcctc
      901 gatgcagttc gctgacgcag aaagcgttat tttcccaaga cccacgatga aaccgtcggt
      961 tgtcacagtt ttttcttcac ctattccgat ccttaggatt tttgttgttt ctgcgttgat
     1021 tcgaccgaag agcccaccag tgactcggag ggtcacactc acgcttgtcg cgttcgcgtc
     1081 cccgatgttc ttcacggtca tggagatttt cccggggctt gcggtgaaat tcccaaggat
     1141 gagtgcggga ttggtcgagc cacccccgtg ggtgacgttg attatccgca gggtgctgtt
     1201 gtcgcaccat cctctcacgg tgttatggcc tctgacactg tagtagtacg tcccaagcgg
     1261 cttatcgatg atatcgtaat aggtattggt aatggtgctt gaaagtatcg aagttgatcc
     1321 aaagattccc atcggtgata tatcgtcgac atagaatcct tcttgatgtc catatccgtc
     1381 tgttgcgtag cggaaccgta tgaagaggga ttttcctgtg aaattatcta cactgtattc
     1441 cttatggatc caatttccag atgatcctgt cagggaatca agcagatggt aattccgtcc
     1501 gttctcgctt acctcaacat aggcataatc ataattttgt tgtatagcat accagcacca
     1561 gaacgaaagg gtcattccag tggtgatggg aacaggtgtc cgggtggtca ttgaagagac
     1621 atcgtattcc cccgtgcgag atttgaagct ctgagcccct gagtgatacc gtgaaggtga
     1681 aagagtgaac ccttcaagtt tccagaaagc agatccagat tcagcatcat cggtggctag
     1741 ggagaggttc ttcatttcgc tcagttgaaa ataatcagga ttggcattcg ggttctgtag
     1801 cgtccaggag acgcggtagt ttccatcggt atcgttttgc atggtcgaaa tgactggtgg
     1861 gatgacgcgt ggtaccaccg tatcccggat gttctgcgct tcttgaagga ggtagattgc
     1921 tccgtcgcag ttctctttac agatctggtt gagataactt gaagccgggt ggaactcgtt
     1981 gcaggtttca atacagtacg caaaggttgc tcttccctga acaaaatggc tgtacccata
     2041 ggcgtaatca atcgtgtccc cgacggttgc gtatagtccg tagctttgat gggggtcata
     2101 ggttcctggt ccgctttgaa tgtttattcg ttgcgcaatc tgttgaccga ccgaggatag
     2161 atacgtattg tcaggagtct gactaccggt gtatccccag ggccacatga ccgtctcgcc
     2221 gtatgtatgg taggttatgg tagcactgat attgttttcc aaaaagatac tggcgaccgc
     2281 ctgattctca gcctcagagg taggtcctgg gccaccgtag cattcattat tagaatcatg
     2341 agaattcgcc caagagggaa tcgaccccca ggagcctaaa atgttgccgt cacaggagcc
     2401 attgtaattc cgatttgtat caacaccata gctgccatac tgcgggaagt aatcccggtt
     2461 cttccgccaa tcgtttcctt gatcgtgaca atagtagtat ccatcagggt tcgtcacagg
     2521 gacgatccat atccttcggt tgtccaccaa gtcagtgata gtcgaatcga caccataaag
     2581 agacgttaga ttattggcaa tgtgcagaca gatttcagca ccaggccatt cacgggcatg
     2641 gtgtacaccc atgtagaaca ccccaggttc cccttcatcg acaccagggt gatcagatat
     2701 ctccagacac cagatgctgc gaccctcgta cgtcgtccca atactataca gggtggcgat
     2761 gtcagagtaa ttagcggcaa tcccttgtaa gatcgtctga atctgagcaa gtgtatgata
     2821 cgaacccgcc atcatggtat cataggcttc cagatcctct atgatgatcg agtagggtac
     2881 attcatctct gaaaattcat gcagcctatc tgacggaata atcacatcaa cccaagcccc
     2941 agggttgtcg cttgctatct cgatattttt aggaagcact ggtacaccag agccagtcgt
     3001 cactcgtacc aacgtatgat gaggagtcaa atcatcgaag agtccaccga aaggactgcc
     3061 tgccccgata ctagggtaag cgaccgtaat catcaataga cacacaaaaa tacctgttat
     3121 ttttctgcta ctgttcatag tatccccgat tcccatatta tgcaagcttc atatataaat
     3181 attcgttgta tgcccccaga gataaaaaga gagtcgagga tcactaaccc tcctcaagta
     3241 gtggcgaaca ctgatgtata ttacgtcatt ttcgactatc atcagacagt ctatgctttt
     3301 gtatacgacc tgcaatgttg cgaacatata aataacaggt tatgctatag acgtaacgag
     3361 accatgaaca gggatgaagc gagccatgga attcagacgg ctcttgagct ggtcttgcct
     3421 ttttcattcg ggtttattat cgcgctttta tttttaagag aactaaaccc ctcggtagag
     3481 aagcttcatt ggttctttgg cagttcgata ggtgcccttg tctcgttact tgggctcatt
     3541 ggccttgtca tcacgttcag aatccagcat attcgcgagc agatcaggga tgtccagagc
     3601 gcgatcctgt cgtactcgaa ggatgggctc tgctgggtat ttgctggttt cgacttcgat
     3661 gaaataacga ggagaatgga ttggcttttt tcacaagctt tcaaaaccca cgaagaagac
     3721 cttaaatctg ataaaaatcc cgaagacata gagtatgacc aggcgaattt ggacgcgtac
     3781 cgtaacgaaa gaatccgctt cggggagatg ctgaaaacct acctagattt ggaaaatgag
     3841 cggaaaggaa tgtatcatat gatggtccct ccaatagtac tgaccgtcgg aacaaccatc
     3901 gtgtcgattt ttgggttgat atccgctgat atcatctcct cggaagggat ctacggttca
     3961 gcatttttcg taggagcagc tgcgctactc atctggctcg tagtcagggt gggcatcttc
     4021 cttataacga tgatgttcaa agaaaggaag attagggttt atgtgaccgt ggacgtcgca
     4081 gatgagaaaa agtaatgatg atgtccataa ttggcctatc cctttgatct ttcttccaat
     4141 gcctctctca tcacttcgta tcaaatctta taccttacgt attcttaaat aacaaaaaat
     4201 gattcaagtt attgtgagcg aaaatgaaca ttagtattat ctatgaatca aaatatggta
     4261 atggaaaaga agcgatgacg tatctagaga atctcttaag caaaaaaggg tattctgtgc
     4321 aactcatgtc agtccacgaa gtcaagccac aatcccctcc tgaatcggat ctttatattt
     4381 ttagcgcacc taatgcattt gggaaaatcg tccgatcaat gagaaaattt ttagaaaaaa
     4441 cagagataaa aaactcatct gcacagtata tcctcgtcaa tacatgtctt aatccatctt
     4501 ccagtcagga caaaggtctt gaacaaatgg aggaaattct taggacaaag aatatctcca
     4561 agttatctga gggattaaaa ctcaaggtta tggcaatcaa aggacccctt gaagcaggat
     4621 atgaaaaaaa gcttgatgag cttgctcaga cgatccagtc ttcccttaag aagtgagtga
     4681 tgcatagtat tctatcgagg cgcactggat ttccctcttt tgaaccaata cgcaagcagg
     4741 gcagtggtaa tcggcatcac aatcgtccaa acaagaagac caccgatcaa aatacgccag
     4801 tccagattca acgcaaaacc gatagcccct cctatgccca agacaccatt gaaaacaaaa
     4861 agcaatcgaa tcgccttctc caactttcct gattgaaaaa tcggagcaat aaaaagcgta
     4921 gaaagcccca taaagaaata tcccaacact tcaatggtca tcatcattga ttgagggttc
     4981 tttatcgaaa atacatcggc gttaaaagca ccaaattgaa caaatgtcaa ttgaatgaaa
     5041 taattcacgc tgatcatcgt cgcgtagatg attgcaaacg aaagcccgat gtgccctaat
     5101 attttcttat cttcaggagc aatagagtaa atacatagaa tgacaattaa aaatatcgag
     5161 gtcaaaataa accctgaaat gttcgatata aggtaaatgg aagcactgga agtgacaccg
     5221 gatattgcta aaaaaatagt cactaatatg gctgaccaga acccaattct actcaccaat
     5281 gtattcataa attgttaatg atatccatca tatatcaata ttatgcatag ggtaaaaaaa
     5341 gaattaattg gaaggagttg aaatgcctaa aaatgaagat gtatatttct cgtatttcga
     5401 gcacaacaaa actatcgcac acgtattatc actattctgt ggctttttgt ttacctccat
     5461 cacaatcctc ctgaccgctc ttaaaaatcc ttcagagaca attgcacaag caacccttct
     5521 atttctaacg attcttttct atttttctct ttttgtgtta atagataatc ttgagatgcc
     5581 gttccattat atcaagaata ttccaccgat gacattaaag atacgcccgt ttttctattt
     5641 gttagtaatt ttctacctct ttggagctac aacggttatg atgtttcttc tttttcatct
     5701 agtatatctg tcattaatct ctgggatgat atgggcaata atcgtactat taaacatctt
     5761 ttcaactgcg aaacgattct atgaacaagc caaaaaaaga aactgggaat cgaatatccc
     5821 taagtgaagt aaaacgactc gcatcgccta tcccaatcgg agcagatctc cggggtatta
     5881 aatccagatg gaaataaatt ttattcccgt tgttttggtt acgggggcat tgattctttt
     5941 ttattagaaa aaaataattt gcgactgaat aattacatag tgcgatgtgt aaacgtaaca
     6001 ataattgaac gatacgctta attctcattt ctggtaaaac taaaaaaagg tgcattaact
     6061 tttaattatg ttcgcaaatg aaacttctta ggattctcaa aagtcacaac ataaacatct
     6121 attgatcccg attctcttag gatgtgatcc atgatatcgt ctttgaataa tcgcttaaac
     6181 caaggatgcc atgttttccc cacaacaatc tttccgattc tattttcccg tgcataatca
     6241 aggattgtat gcggtacaat ttcacctttg agtcttacag cttttgctcc aagtgacctg
     6301 gcaagatcca tattatcatt cagacgttgt gattcctcaa tcgttcgtgt cctttcatta
     6361 tccttcgaag actcgacaga aaccacatcc caatcagtat taagctctcc tgctaatcgt
     6421 gcacctttcc gaattaacaa gcatgcattt ttagaattcg aactaatcgc cactaatata
     6481 cgttctttta gaacctcacc acgagaatct tcagacagag ttgcgtctct ttttgcggct
     6541 tttgcaccaa cactttttgc tacttcacgc aaagagagtt cgcgaagcga ggtgagcttc
     6601 cgtaactgga aaaaattatg aagcgccgtt gcaatcctat ctcttgggta aatcttacct
     6661 tcttcaagtc tattcaaaag ctcatcgaca gggagatcca catcaacgat ctcgtcagcg
     6721 agtgtatcaa ggaatctatc aggaaccgtc tcagtcactc gtactttcgt tgtccgcgca
     6781 atgtcatcgt taagactgtc aatgtgctgg acattcatag tagtcatcac gttgataccc
     6841 atcgccagac attcttcgac atcctgccat cgtttcgcat tatgaccacc gggcgcattc
     6901 gtatgtgcga gttcatcgat cagagctaac tcagggagac gcataagaac tgcatccatg
     6961 tccatatcga agagatcatg ccctttgtag ttaatttttc gttgtgggat ttgttcgagg
     7021 ccttcgagga gattttccgt ttcatctcgg ccgtgggtgt caacgatacc gacgacaaca
     7081 tcaacgcctt tctgctttaa ttggtgcgcc tcctggagca tccgataggt tttaccgaca
     7141 cctggtgcga aaccgaggaa tattttaaga tttcctcgtc cttcccgccg aataaggtcg
     7201 agaaatgact caggacgagg acgtatgtct ttttttattt cacgcataat ggattgctcg
     7261 ataggtcttg aaatatatag tcattcaaca ctatattaat catatgtttg aatcgattct
     7321 ctctcatgct aatccgatag tagtaatcag aatatcaatt aatttaatcc cgataaatgg
     7381 gacgatcatt ccaccaactc catagattaa agagtttcgt cgaaggatac tagatgctcc
     7441 tgctggacga tatgccacac ctttcaatgc aagagggata agcaggatga tgataatagc
     7501 attaaaaata accgcactaa gcaccgcgct ttcagggctg tggagacgca taatattcat
     7561 ggcatgaaaa attggtaact cagggaacat cgccgggatg atagcaaaat atttcgatac
     7621 atcattcgcg atactaaatg tcgtcaaagc gcctcgagtc acaaggagtt gtttaccggt
     7681 ttcaataaca tcaataagct tggttggtga agaatcaaga tccaccatgt taccagcctc
     7741 tctcgctgca tcggttccgc tattcatcgc aagccctaca tccgcttgag caagagctgg
     7801 agcatcattg gttccatcac caatcattgc caccaactga cctttcgctt gttccttacg
     7861 tatgtacccg agtttatctt ctggttttgc ttcagcgata aactcatcga cacctgcttc
     7921 atccgcaatg gtttttgcgg taatgggatt atcacctgta atcatgacgg tccgaattcc
     7981 aactcgtttc agaatacgca atcgatctgc gatcccaggt tttacgatat ccttcagatg
     8041 gatgactcca acaatacgtt cattgtctga gaccataaga ggagtaccgc cttcccggga
     8101 gatacgtatg accgcctcat tgacctctat tggtaccgaa ccacctaagc tatcaatata
     8161 tttcgtcact gcctcaggtg cacctttgcg aatcttccta tcagggaggt caattccact
     8221 cattcgtgta tctgcactga aatcaatagg taccccgctc agtgcttcct tctgtgaagc
     8281 tccctgagca agagttaatt caacgatact ccggccttca ggggtgaaat ctgaaagaga
     8341 gctcaataca cacatatccc gaatctctgc attactaact cctggcgcag ggatgaactc
     8401 aactgcttgt ctgtttccca gggtaattgt tcctgtttta tcaagaagga ttacatcgat
     8461 atccccagct gcctcaacgg cacgcccact ttttgctacg acatttctgg caagtaaacg
     8521 atccatcccc gcaataccaa tagcactaag aagtgcccct attgttgttg gaattagaca
     8581 aacaagaagc gagataagaa cgacggtact tacatctgtt tctgtgtatg atgcgaacgc
     8641 gacaagagta acgacaacaa ccatatacag aagggtaagt cctaaaatga gaatagtcag
     8701 agcaatttca ttaggagttt tttgtcgctc agcactttca accaaatcga ccatgcgatc
     8761 catgaatgat tcaccaggat caacggagac tttgacccgg atactatcgc tgagaacgcg
     8821 tgtaccacca gtcaccccgc ttttatcgcc accgctttct cgtatcactg gagcactttc
     8881 accggtaatg gcactttcat caacacttgc gacaccttcg tctactgtgc cgtcagccgg
     8941 gatgatatcc cccgcggtaa ctagtacaat atctcctttg cgaagatctg ttgcgttcac
     9001 gactcggatt tccccgtttt tattaatgag ttttgcttga gtatctactt tcgtttgtcg
     9061 taagctttcc gcttgagcct ttcctcgccc ctcagccatc gcttctgcaa cattcgcaaa
     9121 tattaccgta aaccacaacc agatagctat ctggatagga aataaaaaaa catggccagt
     9181 actgagatta aacaaggaca ccaacgaaac aaggattgca ccgacttcaa cgacaaacat
     9241 gaccggattg tgtaattgaa ttcgtggatt caatcgtaga attgattgtt tcaaggagaa
     9301 ggagattatt tttttatcaa gaagaggtct tgtacgagct gctctgcgga tttctttttg
     9361 atcttgcgtc atcgtatgtc tatcgttgct tccgtttttt atcaaataaa taaaccccca
     9421 cataagaaaa gctttccgcc gaataataag agatgctcaa gaatcggacc aagaactaaa
     9481 atagggagga aggataagcc tgcaacaatg atgatgaccc cgatgagaaa tgccccgaag
     9541 gtggcgttat cggtcggcag agtccctgct gttggtggat aaatgtactt ctccgcgaaa
     9601 ctccctgcga tggcaagcat tggaacgatg atgaaaaacc tcccgatgaa catcgcaaga
     9661 gccatcatga tgatgaacca aaacgacgca gcattaagac ctgcgaacgc actcccgtta
     9721 tttccaacac cactcccaaa tgcgtagagg atctggctga gcccatgagg ccctgggttt
     9781 gtgatcgatg acaaaccatc gggggttacg acagataacg cagcaaaaac aagaatggtt
     9841 gtctcaatgg aaataagaat gagtaccgtc atctttatgt cgaaagatat gattttcttc
     9901 cctaaatagt ttggaatacg cccgaccata agaccagcga taaatatggt aatgagcacg
     9961 tacatgaaca gacagaagaa accagctcca acccccccag gaactacttc acctaagagg
    10021 attaaaacca gagggatcat tcctccaagt ggtgtatatg aatcatgcat gctgttgaca
    10081 gcaccgcatg ctgttgatgt cgtagcgatc gcaaacatgg tagagccata agtaccaaaa
    10141 cgaacctctt ttccctccat gtttccccct gcttgaagac cactcggtga ctgatcaact
    10201 cctaacgtat tcagcgagcg atttcccatt tgctcctcaa aagtggtcac cgttaccgca
    10261 gcgataagaa aaattaggac gacaataaaa agtaatacac cttgtttcaa ttgacgtgcc
    10321 atacgtccga acatgactaa aatagacaat ggtagtaaaa gcagcaggaa tatctggatg
    10381 acattagaaa agggggttgg attctcaaag ggatgagcac tattcgcatt aaaaaaacca
    10441 cctccatttg taccgaattc tttaattgct tcctggctcg caacaggacc aaccggaata
    10501 acttgttggg caccttcaat agtagtcgca tgaatagggc cattgaatgt ctggggcacg
    10561 ccttgactga caaagaaaag agcagcgacg atgctgatag gtataattac gtacaggacg
    10621 atttttgtca tatcgaccca gaaattacct aggcctttgc ctttttttcg tttcgtgata
    10681 gcacgaataa gcgctaaagc gacaacaatg ccaaccgctg cactcataaa attctggaag
    10741 gtcaacgcaa gcatctggct cagataactc atggtctgct cgccagcata cacttgccag
    10801 ttcgtattag ttccaaagct cattgcagtg ttaaaggcaa catcaggttt cacactggtt
    10861 aatccttgag gattgagagg aagcgatcct tgtatccgta aaatcgcgta cgtgaataga
    10921 aaggaaatga agctgaatgc aagcacacaa aaggcgtaag tcttccagtt catatcttct
    10981 tttgaatcga tgtgtaacat acgatagaac caggattcta cgcgttcaaa tctttttgtt
    11041 attttcgttg gtcgccccgt aacgatatta taaagaaacg tcccgagaaa atatgatgta
    11101 atgatgagaa gaacaaaaat gacgactgct tgaatgatgc cgaagaattg aggattcccg
    11161 tgatcaaagg taaagacatt ggcacttgga acagtcttac cagtatctaa ggcgagattc
    11221 agtttgagaa cattgatccg tggttcacca aacaccccta aatctcgacc ttcaacgtac
    11281 tgcgcgacga cacctctgag ggtttcctca gatatattcc gagcttttgc gactcttgag
    11341 atctggtcca aggcattcgc gagagttata tgaggatcca aaccactacc gctggtcgta
    11401 accgcatcaa tagggatagt accatctttg tttgtctctc cacccaagcg actttcgttt
    11461 atctctgttt gaattcgttg acgtaaaagc ggattggatg gaccaagatt actaccgcca
    11521 gaaaacatcg catcgtaacc agcaccagca ttactgggac gactatggaa atatttatca
    11581 tgcgtgaaat tctgaccaat aagttcgctc ccaataatag tctcattatg aacaatgata
    11641 ctgccgttgg cttggtgagg aaatgccacc tgggcaacac cggtgataaa tgccgggtag
    11701 gcgagtccag taataacagc aaaaataacg gtagaaagga ttgcaggtcc aatatatttt
    11761 ttcagaacat ttcgcatacg atcgcctcag aatttttctg gttgaagaag tgcatataaa
    11821 agatagagga atagcaccgc acaaaccaca attaaaataa taacgtctat tggcatctaa
    11881 aatcccccct atagcctctc acaggctttc gcatacgccc atgctgccag gaataatccg
    11941 gctaaaatca gaagaacgac ccaatcatcc ataccggtca cctcaggatg ttcggggctc
    12001 agctatctct catgcccttt atacgttttt cgacagtgta agaacaggat attacaagga
    12061 gtaaacaaga tgaatagcat cttggatgcc tttgataata a
//
LOCUS       DATMXJ010000179         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_70982, whole genome shotgun sequence.
ACCESSION   DATMXJ010000179 DATMXJ010000000
VERSION     DATMXJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70982"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VN377_04670"
     CDS             complement(<1..343)
                     /locus_tag="VN377_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HWR27714.1"
                     /translation="MKRRIHPFQSLFFIFFILYMIWVFLLFLAPLVLPSGSVSDLSGV
                     VGFSDNAAVINDLPFPWNAVYTFGDLFCHQKAERSLFLNGNEMPFCTRCTAIWLGLAF
                     GLGFMILFTIEL"
     gene            450..599
                     /locus_tag="VN377_04675"
     CDS             450..599
                     /locus_tag="VN377_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HWR27715.1"
                     /translation="MTRFPEAESRLLNKKICMKCGASNAIKAERCRRCRSTELRAKAK
                     EGRGQ"
     gene            660..2078
                     /gene="proS"
                     /locus_tag="VN377_04680"
     CDS             660..2078
                     /gene="proS"
                     /locus_tag="VN377_04680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011012437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWR27716.1"
                     /translation="MPETKVKKSDDFNEWYNEIVELADLCDKRYPIKGMNIWRPYGWK
                     LMNHVDRLIRDEMIRTNHQEVCFPLLIPESLFKKEEEHIEGFGKEVFWVTHAGLNQLE
                     ERWLLRPTSETAMYPIFALWIRSHTDLPLKTFQIVNTFRYETKQTRAFIRVREIHFFE
                     AHTCHVDFEDAERQITEDKEIANRLLDKLCLPYVFSKRPEWDKFAGAYYTISIDVLMP
                     SGRTLQIGSIHQYRDNFAKPYEISYETEKGTHAFCHQTTYGMSERLLGALIGVHGDNK
                     GLIMPPEVAPLQVVIIPIIFKGKEQAVLETSSSLHKKLLEEHIRSSIDDREITPGNKY
                     YDWELKGVPLRIEIGPKDVEKNELTLVRRDSSKKKSIPLKDAEKTIKTELQDLEQSLY
                     ANAEKLLKENMHCVLTIEEAKQKTGIVALPWCGNKDCALEIENVLEGNTLGEPIENAG
                     CSEHCPVCGLPAKTWMRYAKSY"
     gene            2091..2483
                     /locus_tag="VN377_04685"
     CDS             2091..2483
                     /locus_tag="VN377_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27717.1"
                     /translation="MAGRITGFLREYVLALSILTIIPGIVFIIMGAIFLFFNSIGEPA
                     NSLLHFIFVLGVWNDYILIAGLIIFGIGVYYLYSYITKRKYVLDELKTDKRSEILKKR
                     SKLESTVKHLPSKYQKMLSDKEEECNIR"
     gene            2494..3225
                     /locus_tag="VN377_04690"
     CDS             2494..3225
                     /locus_tag="VN377_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWR27718.1"
                     /translation="MAEKKPLSFFYVVLVEPKNSGNIGAVARAMMNFDAQHLYLVNPC
                     ELDNVCYARAMHATKILDDAKTFSCFEDAIENLDYLVATSSIESKTDKRHLRNPMLLE
                     DFSEKIFDVKGKVGLVFGREDYGLFNEEIAGCDIMLKIPTSESYLSLNLSHAVTIVLY
                     SIYLKKALIPERRRVIGSLEKQKLFEFFAELLDDIEYQPHKKEHTKIMFKRIMGRAMP
                     SKWEYHTLMGVLSKTLGKIKQKEKK"
     gene            complement(3231..3455)
                     /locus_tag="VN377_04695"
     CDS             complement(3231..3455)
                     /locus_tag="VN377_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27719.1"
                     /translation="MKTYIQVMFNSEGAAPSEIKNQLLNMGFKATKGNYDFVFEWGKE
                     KADINELIFFADKVHATLKNSKVLFNIETI"
     gene            complement(3544..>4211)
                     /locus_tag="VN377_04700"
     CDS             complement(3544..>4211)
                     /locus_tag="VN377_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWR27720.1"
                     /translation="MGHYYASKKHHVDASLPYFIPLPPPFILGTFGALISTREPIPNR
                     KALLDIGIAGPLCGFLVAIPISLIGFFLMQQQPLPIPSNGVNISLVPPLLLQWMQNLF
                     SMSGNYIIHPTLFAGWVGIFLTAVNLLPAGQLDGGHVARAILKEKHKYISWVVIFVLA
                     GLSFFYTGWLMFAIIILLFIGTQHQPPLNEITPLDSRRKLLGLIILIIFILSFAPIPF
                     AAG"
BASE COUNT         1433 a          863 c          870 g         1045 t
ORIGIN      
        1 ttaactctat tgtaaaaagg atcataaacc caagaccaaa agccaaccca agccaaatag
       61 cagtgcaccg agtacaaaac ggcatttcat tgccatttaa gaacaaagaa cgttcagctt
      121 tctgatgaca gaatagatca ccgaatgtat aaacagcatt ccagggaaac ggcagatcat
      181 taatgacagc agcattatct gagaaaccca caacaccaga aagatcagaa acactcccag
      241 atggtaaaac aagtggtgcg agaaacagga gaaaaaccca aatcatatag aggataaaaa
      301 agataaaaaa cagactctga aagggatgta tccgtcgttt catcgttcaa cctaccatac
      361 aagatggctc atattcatta taacaacgag agaagggcat catatttaaa tatgatatgt
      421 ttattctcga tacacctagg cgataaccga tgacccgatt ccctgaagct gaaagccgat
      481 tattaaacaa gaaaatctgc atgaaatgtg gagcatccaa cgcaataaaa gctgaacgat
      541 gccgacgatg caggtcaacg gaactacgag caaaggcaaa agaaggcaga ggacaataga
      601 atcaaaccag tatctttaaa aacaagatac ctctttttgg ttgaaacagt ggaaaggcta
      661 tgccagagac aaaagtaaaa aaaagcgacg atttcaacga atggtacaac gaaatcgtcg
      721 aacttgctga cttatgtgat aaacgctatc cgattaaagg aatgaacatc tggagaccct
      781 atggatggaa actgatgaac catgttgacc gactcatcag ggatgaaatg atacgaacca
      841 accatcaaga agtgtgtttc ccgctattaa tccctgaatc gctcttcaaa aaagaagaag
      901 aacatatcga aggattcgga aaagaggtct tctgggtcac ccatgctggt ctcaatcaac
      961 tggaagaacg atggctcctc cgaccaacat cggaaacagc catgtatccg atcttcgcac
     1021 tgtggatccg ttcccacacc gatttaccgt taaaaacctt ccaaatcgtc aatacattcc
     1081 gatacgaaac gaaacagaca cgagcgttta tccgtgtacg agagatccat ttcttcgagg
     1141 cacatacgtg tcatgttgat tttgaggatg cagaacgaca gataacagaa gataaagaaa
     1201 tcgcaaaccg tctcttagac aaactctgcc tcccctatgt ttttagcaaa cgaccagagt
     1261 gggataaatt cgccggagcg tattatacta taagcattga cgtactgatg ccatcaggaa
     1321 gaacattaca gattgggtcc attcatcagt accgtgataa ttttgctaaa ccttatgaaa
     1381 tctcctatga aacagagaaa ggaacccatg cattctgcca tcaaaccacc tatgggatga
     1441 gcgaacgatt actcggcgca ctcatcggtg ttcatggaga caataaaggt ctcatcatgc
     1501 cccccgaagt tgcacctctc caggttgtga ttatccctat tatatttaaa ggaaaagagc
     1561 aagctgtcct cgaaacaagc agttcgttac acaaaaaatt actcgaagag cacatccgat
     1621 ccagtatcga cgatcgggaa ataacaccag ggaataaata ctacgactgg gagctaaaag
     1681 gagtaccgtt gagaatagaa attgggccaa aagatgtgga aaaaaacgaa ctgactctcg
     1741 tacgaagaga tagtagcaag aagaaatcaa tcccactcaa agacgcagaa aaaacaatca
     1801 aaaccgagct acaagacctt gaacaatctc tctacgcaaa cgctgagaag cttctcaaag
     1861 aaaacatgca ttgcgtacta acaatagagg aagcaaaaca aaaaaccggt attgtcgccc
     1921 taccctggtg cggcaataaa gactgtgcgt tagaaataga aaatgtttta gaggggaaca
     1981 cattagggga gcccattgaa aacgcaggtt gctcagagca ctgtcctgta tgcggcttac
     2041 ctgcgaagac atggatgaga tatgcaaaat catactgagg tgaaagaaaa atggctggaa
     2101 gaataactgg gtttttacga gaatatgtcc ttgcgttaag catcctaact atcattcctg
     2161 gaattgtttt tatcatcatg ggtgctatat ttttattttt taacagtatt ggagaaccag
     2221 cgaattctct cttacatttc atcttcgtcc ttggcgtatg gaatgattat atcctcatcg
     2281 cagggttaat tatctttgga attggcgtgt attatctcta tagctacatc acaaaacgga
     2341 aatatgtact tgatgagttg aaaaccgata agcgttcaga gatccttaaa aaacgaagca
     2401 agcttgaaag cacagtaaaa catcttcctt cgaaatatca gaagatgtta tctgacaaag
     2461 aagaagaatg taatatccgc tgatcccatt gtcatggctg agaagaaacc tctctctttt
     2521 ttttatgttg ttcttgttga accaaagaat agcggtaata tcggtgcagt tgctcgagcg
     2581 atgatgaatt ttgatgccca acatctctat cttgtcaacc cctgtgagtt ggataacgtc
     2641 tgttatgctc gtgcgatgca tgcgacaaaa atccttgatg acgcaaaaac attttcatgc
     2701 tttgaagatg cgatagagaa cctcgattac cttgttgcga cctcatccat tgaatcaaaa
     2761 acagacaaac gacatctgag aaatcctatg ctccttgagg atttttcaga aaaaatcttc
     2821 gatgtgaaag gaaaggtcgg gctcgtgttc ggaagggagg attatggttt gtttaatgag
     2881 gagatcgctg gctgtgatat catgctgaag atcccaacga gcgaatccta tctgtctttg
     2941 aacctttccc atgcagtgac gatcgttcta tactctatct atctgaagaa agcattaatc
     3001 ccagaacgcc gccgagtcat tggatcactt gaaaaacaaa aattatttga attctttgcc
     3061 gaactactcg atgatattga ataccagcca cataagaaag aacatacaaa gataatgttc
     3121 aaacgcatta tgggacgtgc gatgccctcc aaatgggaat atcatacact catgggtgta
     3181 ttaagcaaaa cccttggaaa aatcaaacaa aaggaaaaaa aataaggttc ttagattgtt
     3241 tcaatattaa agaggacctt actattcttc aatgtagcat ggactttatc agcaaaaaaa
     3301 atgagctcat tgatgtctgc tttttccttt ccccattcaa acacaaaatc atagtttccc
     3361 tttgtcgcct taaaccccat gttcaataac tggtttttta tttcagatgg agcggctcct
     3421 tcactgttga acataacctg gatgtatgtt ttcatgctgt tcacgtcaag ataaggattc
     3481 agtatgaggt attaaatcat tacgatgcaa tcgttggtga taataaggag aaaaacaggt
     3541 cctttaccct gccgcaaagg ggataggtgc aaatgagaga ataaagataa taaggatgat
     3601 cagaccaagg agcttccttc tcgaatccaa cggtgttatc tcattgagag gcggttgatg
     3661 ctgagtacca atgaagagca agataattat cgcgaacatc aaccatcctg tgtaaaaaaa
     3721 actcaatccg gccaggacaa aaataactac ccagctgatg tatttatgct tctcctttaa
     3781 tatcgcacgt gcaacatgtc caccgtccag ttgacctgca ggtaagaggt tcaccgctgt
     3841 aaggaagata ccaacccatc ctgcgaacag ggtcggatga ataatatagt tcccagacat
     3901 ggaaaaaagg ttttgcatcc attgaagtag taacgggggg acaagtgaga tatttacccc
     3961 gttcgatggg atgggaagtg gttgttgctg cattaagaag aaccctatca aagatattgg
     4021 aatagcaaca aggaatccac acagtggtcc tgcgatccca atatcgagca atgccttccg
     4081 gtttggaata ggttcccttg tcgagattaa cgccccgaaa gtcccgagga taaacggtgg
     4141 tggaagagga ataaaatatg gtaacgatgc atcaacatga tgtttttttg acgcatagta
     4201 atgtcccatt t
//
LOCUS       DATMXJ010000180        11872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_71009, whole genome shotgun sequence.
ACCESSION   DATMXJ010000180 DATMXJ010000000
VERSION     DATMXJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71009"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VN377_04705"
     rRNA            complement(<1..1074)
                     /locus_tag="VN377_04705"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            complement(1228..1299)
                     /locus_tag="VN377_04710"
     tRNA            complement(1228..1299)
                     /locus_tag="VN377_04710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ala,seq:tgc)
     gene            complement(1406..2874)
                     /locus_tag="VN377_04715"
     rRNA            complement(1406..2874)
                     /locus_tag="VN377_04715"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(3270..3797)
                     /locus_tag="VN377_04720"
     CDS             complement(3270..3797)
                     /locus_tag="VN377_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWR27721.1"
                     /translation="MPKSSLTRLIEDEEKILDILQINAQASIDDIAKKCGFSRQKVWR
                     IIKKLEEEKTIWGYTAICDQERYNLKHFVMLMKRNTTPISQKIMQEILSTRLDDLIPD
                     SIIHIEDIEYVHGSYDGIFSFLAPDLITAKKFCDRFKETFNEYVGGVELLEGIFFIRN
                     QRLRNPNLDKIVHYL"
     gene            complement(3889..3960)
                     /locus_tag="VN377_04725"
     tRNA            complement(3889..3960)
                     /locus_tag="VN377_04725"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3926..3928),aa:Ala,seq:cgc)
     gene            complement(4018..5256)
                     /locus_tag="VN377_04730"
     CDS             complement(4018..5256)
                     /locus_tag="VN377_04730"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWR27722.1"
                     /translation="MKEYNTLDDFSVENKTVLLRVDFNMPLDKKTLNITDDTRIRLAL
                     PTIQELMEKKAKTVILAHQGKQGSWDFISMQKHAEALQKLLGKPVTFVDDIYGEKAKH
                     AIQIMKPGAVVFLDNVRKYPAETEKKKPEEHAQSDLVKNLAPLADLYVNDAFAAAHRA
                     QCSLTGFTAVLPSAAGRLMEKELTSLEKVIQRPEKPSVFLFGGAKFADGIVTIDRLLT
                     NKTADHVLLTGLTANAFLKAKGVNLGKKNEETFGEEGTPEIFTEIKNIYKKFEKQIHL
                     PIDFAIEENGKRKEIPLADLPTEHNLFDIGEKTIQQYKKILEPAKTIFISGPCGVYEN
                     PLFRKGTEEIFKCITQSKAFSIAGGGHTVAAIEQMKLREKISHISTGGGALEKFMMGE
                     KLAVVEALKTAKQRQLIQVK"
     gene            complement(5359..6405)
                     /gene="gap"
                     /locus_tag="VN377_04735"
     CDS             complement(5359..6405)
                     /gene="gap"
                     /locus_tag="VN377_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWR27723.1"
                     /translation="MIRVAINGFGRIGRNVLRSAVQHSQYGKKFEVIAINDLGNTEIL
                     AHLLKYDSIYGKFCGDVAAKPEGVEVNGHLIAFFSEVNPTNLPWGKLDIDVVIESTGV
                     FRTREGAGKHLEAGAKKVIISAPGKDPDLTIVLGVNEKMYDPSKHHIISMASCTTNSL
                     APPVKVLHEKFGIEQGFMTTVHSYTGDQRILDFPHKDLRRARAAAVSIIPTTTGAAKA
                     VSLVIPDLKGKIDGIALRVPTPDGSITDFSCVLKTPTTTQEINAAFKQASQTTLQGIL
                     QYTEEPLVSVDIVGNPHSAIIDGLMTKMIGEKGNFAKIFSWYDNEWGYSCRIVDLVNY
                     IFNERMVPIETYKN"
     gene            complement(6439..7689)
                     /locus_tag="VN377_04740"
     CDS             complement(6439..7689)
                     /locus_tag="VN377_04740"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HWR27724.1"
                     /translation="MNIYVETYGCTANKSDERLLLGLLQQKGHQIVPDISGADVLVLL
                     TCTVIATTEQRMLSRLQVFHKTHKKIIVTGCMPAVQKDLILSVAPQATLLSSRYIQYI
                     NDIINGDNPVFVETKKTTLPKYYDTALAPILISEGCRLSCSYCITHFAKGALRSFAAG
                     EIVTDVCSALKQGCKEIQLTAQDTASYGLDTGTNLGTLLKRISTLDGAFRIRVGMMNP
                     LTLQKNLDSVLKAYQHPNIYKFLHLPVQSGDDEILQKMNRGYTIKEFTSLIHHFREAI
                     PSLTLSTDVIIGFPTETDEQFDRTIELLKEIRPDIVNITRFSARPLTLAKKMKGRIPT
                     HVVKTRSKQATEVCAKLSLEKNKQHLGKTYSVLVTEPGKKKTVTGRSDSYKQVVLTEP
                     VPIGSFVQVKIIHATSTYLVGKLI"
     gene            complement(7715..9265)
                     /gene="lysS"
                     /locus_tag="VN377_04745"
     CDS             complement(7715..9265)
                     /gene="lysS"
                     /locus_tag="VN377_04745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWR27725.1"
                     /translation="MHWADVLAEELLKKQTKHVLATGITPSGPIHIGNMREILTTDAV
                     YRCLIEKGGDADFIYICDDYDPLRKVYPHLPSSYQQYVGKPLSEIPCPCGEHTSYADH
                     YLTSFLGSLHTIGVNPRVYRASEMYYKGDYNESIQIALENTTKIRNILETLSGRKMPN
                     DWLPYNVKCETCGRLSTTHPSLYEFPNVEYTCDCGHDGEVDIRKGGQGKLPWRVDWPA
                     RWRMLGVTFEPCGKDHATVGGTRETGAKISEEVYHYPPPGVLVYEFILLKGMGAMHSS
                     KGTAVSGEEMLRMTPPEVLRFLIMRNQPNRHIVFDSGLGLLTLVDEYDTEEGVYFGKE
                     SEIKGMKEFKKIYELSQPCHIPKKMPLHLPYRHLVTLNQIAKTWPEVKEILMRTGQLT
                     KKLTKEDEEHLRQRAEHVRYWLATFAPDEVKFEVKETSPTITLTEEQRTILSIFKEKI
                     PDLSWEPEAIHNAIYEISEQTKIPIKTAFSAIYKILLGQERGPRAGFFLSNLSKQFVL
                     NRVTEAVK"
     gene            complement(9291..9872)
                     /locus_tag="VN377_04750"
     CDS             complement(9291..9872)
                     /locus_tag="VN377_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HWR27726.1"
                     /translation="MKETDSLIKKALKFKESGLTDYEIAEELNVSKETAAWLLTRGKD
                     KKPEGELKVGWRSIGVSPTRIGSISYALSDIILEEMEKKEVDVEVVVGIAINGIPFAT
                     YVAEELGLDLAVFRPHHEKTGAFGSNYATIANKNVVIIDDVIGTGETLKSAIKAVKAE
                     KGNPILCVCLLNKRSMNDVGGVPLRSLIRARAL"
     gene            10005..10172
                     /locus_tag="VN377_04755"
     CDS             10005..10172
                     /locus_tag="VN377_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HWR27727.1"
                     /translation="MAQEKKGEGFHSGAGLIRYFDAEEKTAFKIPPWFVVFACIATAV
                     VVIAVQLWKPL"
     gene            complement(10193..>11872)
                     /locus_tag="VN377_04760"
     CDS             complement(10193..>11872)
                     /locus_tag="VN377_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWR27728.1"
                     /translation="LGYLGKEFPSLKKRENQISEILDIETRRFSETLSKGEGLIKRIL
                     KEKQTIDPPALIMLYDTHGMPPAVVQNIAQKENISVSIPPNFDSMIAELHSHEKGDEE
                     IQEVQTNLPQTHPLYYDDHYTKEFDATILWKQTTLRGTEVILDKTAFYPEGGGQPADK
                     GELVIDGKSIQVLHVEKKAGSIIHVLDSPAEVGKKVHGKIDWDHRYTLMKHHTGTHVV
                     NGALRRLFGEHIWQAGSQLALTEARFDFAHYKPIAEDEIQKIESIANEFIRQAVPVEK
                     KVMKRNDAERAYGFRLYQGGVPPGNLIRVLNIPGIDVEACGGTHLNNTKEIEKVRILK
                     AERIADGVNRITFAASKMADAYQNEENKLYHRVTDLLSSTYKITGHESIAEQLRETEK
                     FFSVSADQLEKTVQRFLIETNKKNITTVASLVDACNHLFNEWKNAQKQKKMVSTDVVA
                     QLIQGASQIPGTSIRMVSGICDSDAITVAGAIIKESGFIVHIYDGKKLVSAASDNVDI
                     DLRPLASEIGKILGGSGGGKPKLTQCGGPNKTKIHDALEKAKELTLQQIKK"
BASE COUNT         2886 a         2776 c         2559 g         3651 t
ORIGIN      
        1 gctgggttgt ttccctttcg gacatcaagt ttaccccaga tgccctcact accctgattc
       61 tacgatgaat acacattcgg agtttaacaa ggcaccgagg aatttcttcc cctaagcgcc
      121 caattagtgc tctaccgtgt actccatctc cacagagatc tacctgcggg tagtctcggg
      181 gggaaccagc tattgccagt ctagattggt cttttgcccc tatacgcagc tcatctcaac
      241 gatttgcaca tcagtacgag ttcggtcctc caccccactt tcatggggct tcaacctggc
      301 cacgcataga tcggctggct tcgggtctcg tggtaatgac tacaggcgag cacaccttgt
      361 cccttgttgc ctgcggacac ttgctttcgc tgcggcttcg ggccttaagc ccttaacctc
      421 gccatcacca ggaactccct gggtcattat tcaaaacgaa cgacaagaca ttgctccact
      481 atctaagata gcttcgtcgc tttcatgcct tatcagtctg taaccatttg atttcatgct
      541 ctttgcacct cccgctaggg gtactttgca acgttcactc acgctactat tgcactatcg
      601 gtctcgagta gtatttaggg ttggaagttt atgcctccca tattcacgcg cgatatccaa
      661 cgcacgttac tctggatacc caaaaatctc ccactggttt acctctacag gactatcacc
      721 ctctatggta cctctttcca gaggacttcg agtttgccag ttagggagta aatgagtcca
      781 aactccacat ctcccttagt tcgtcactaa gggattcggt ttgccctgtt gggtgttcga
      841 tcgcctttaa taacccaatc tcaattgatt tcttttcctc tgcctactaa gatgtttcaa
      901 ttcggcaggt tcctcttcgc tgacgcgaat atcgccgaag cgataggatg tccaattcgg
      961 taatctccgt atcaaaggct ccttgcgcct acacggagta atatcgcggc ttggcacgac
     1021 cttcatcagc tctcgagccg agcaatccac caaatggcgt agcactggct caatttacgt
     1081 ccaggttgcg tatgcaatcc attgcatgta attcgcaatg ggacgatcat caaccgcatc
     1141 tgcgaagatt gggtctacgt ccttaccatc caatctacca acaaactaga tggcgtgttc
     1201 ttactttttt gaaatggaaa atcggaatgg accggatggg atttgaaccc atggcctcct
     1261 gcttgcaaag caggcgatct tccgctgatc taccggccct tacaataata tgttaagggt
     1321 aaggaccgag gaaattcgat aaaccttaat atactttttg atcgacgtga ttcgagtgtt
     1381 taattcaccc atcaatgtta atcgtaggag gtgatccatc cgcaggttcc cctacggata
     1441 ccttgttacg acttagcccc gctcactaag cctaagtttg aacgcagcaa ctagctgcat
     1501 cctcactcag acccaattcg catggcttga cgggcggtgt gtgcaaggag caggggcata
     1561 ttcaccgcgg aatgttgatc cgcgattact acggattcca acttcatgag ggtgagttac
     1621 aaccctcaat ccgaactatg actaggtttc gggattacct tcacctttcg gtgtcgaaac
     1681 ccattgtcct agcctttgta gcgcgcgtgt agcccggggg attcagggca tactgaccta
     1741 ccgttgccct cgccttcctc tgacttatcg ccagcggttt ctctagtgtg cctagtaagc
     1801 gaacttacca gttgcaacta aagatgaggg tctcgttcgt tgtctgactt aacagaacgc
     1861 ctcacggtac gaactgacga cggccatgca ccacctctcg gaaaatcagg caaggtcatc
     1921 agcctggcct tcatataacc gtcgcccccg gtgagttttc cggcgttgaa tccaattaaa
     1981 ccgcacgctc cacccgttgc ggtgctcccc cgccaattcc tttaagtttc agtcttgcga
     2041 ccgtactccc caggcagctc gcttaacaac ttctctccga cactagttgg gctcggagcc
     2101 cgactaacat caagcgagca gcgtttacac cctggactac cggggtatct aatcccgttt
     2161 gctccccagg gcttcgtccc tcaccgtcag acccgttcta gctagacgcc ttcgccactg
     2221 gtggtccccc taggattaca gaatttcacc tctaccccag gagtacctct agcctctccc
     2281 ggtcccaagt tgaacagtct acccggaatt cgtacggtta agccgtacga tttacccagg
     2341 catttataga accggctacg gacgttttag gcccaataat agcggccacc acttgagctg
     2401 caggtattac cgcggcggct ggcacccgtc ttacccagcc cttactctct ctgtttttta
     2461 gacagagaaa cagctgactc ttacgagcca gcacttggga ttccctcgtc gtgctttcgc
     2521 acagtgcgaa gttttcgcgc ctgctgcgcc ccgtagggcc tgatatcgtg tctcagatat
     2581 catctccggg ctaaagctct cactacccgt atccgtcgta ggctagtcag gtagttaccc
     2641 tgactacaac ctgatagtcc gcagacttat ccttaggcga taaatctttc aatctaaaag
     2701 cattccagca gtctagatct atggagtatt attctcagtt tcccgagggt atcctccacc
     2761 taagggcaaa ttatctacgt gttactgagc cgtctgccga gggccgaacc ctcacgactc
     2821 gcatggctta gtcgaatccc aatagcagtg acctctggca ggatcaacca gaatctggca
     2881 cactattatt agttattatg aattggtggg tgaattaaat atcaccaaca cgtcgatcgt
     2941 cagattcaag agtgcgcaag acgtctacct cagagcatct tgattttctt gaacctccac
     3001 atttattctg ttccccagat ttgaaatggc ctactcatcg aatgtcgcga tgacatactc
     3061 tcataggccg accggaaaga tccgggacag tatatttatg aggttattag ttgaggtaac
     3121 ctcacggtat ataattatca tatataaacc ttacatatat ttcttgcgct tgcatcaccc
     3181 gaagttttaa atatagatat aagttgtcgt cgcgcactgt cgatagaggt tttttataaa
     3241 ataatttttt ttaatgcggg tggaattgtt taaagataat gtactatttt atcaagattt
     3301 ggattgcgta acctctgatt tctgatgaaa aaaataccct ccaagagttc aacacccccg
     3361 acatattcgt taaaagtttc tttgaatcga tcacagaatt tttttgctgt gatgaggtct
     3421 ggtgcaagaa atgaaaaaat tccatcgtaa gaaccatgga cgtattcaat atcttctata
     3481 tgaattatag aatccgggat aagatcatcg agtcgagtcg aaagtatctc ttgcataatt
     3541 ttttggctaa ttggtgtagt gttccgtttc attaacatga cgaaatgttt taaattgtat
     3601 cgttcctgat cgcaaatagc ggtgtacccc cagatagtct tttcttcctc aagttttttt
     3661 ataattcgcc agactttctg tcgtgaaaaa ccacattttt ttgcgatgtc atcgatactt
     3721 gcctgtgcat ttatttgcag gatatcaaga attttttctt catcctctat taaacgtgtt
     3781 aaactacttt ttggcattat tactccattt actgtaaata cggacgtagt agataaccac
     3841 ctaaaattaa aaaaggtttg tattgctgtt tttttaaaaa aaataggatg gactgagcgg
     3901 gatttgaacc cgcggcctcc gccttgcgaa ggcggcgatc ttccgctgat ctatcagccc
     3961 gtacttggaa caaggataaa aaagaaagag tatatgattg tttcctttga atttttttta
     4021 tttcacttgt atgagctgac gttgttttgc tgtttttagc gcttcgacga ctgcgagttt
     4081 ctcacccatc ataaactttt caagggcgcc gccaccggtg ctaatatggg atattttttc
     4141 gcgtagtttc atctgttcaa tcgcagcaac ggtatgtcca ccccctgcaa ttgagaaggc
     4201 tttcgattgg gtgatgcatt tgaagatttc ttcagttcct ttcctgaaca aaggattctc
     4261 gtacaccccg cagggtcctg agatgaaaat cgtctttgct ggttctagga tttttttata
     4321 ctgctgaatc gtcttttccc ctatgtcaaa aagattatgt tctgttggaa gatccgctaa
     4381 cggtatttct tttcgtttgc cgttttcttc tatggcaaaa tcaatgggga gatggatttg
     4441 tttttcaaac tttttgtata tatttttgat ttctgtgaaa atctctggtg taccttcctc
     4501 accgaatgtc tcttcgtttt tcttcccaag gttgactcct ttggctttca agaatgcgtt
     4561 tgcggtcaac cccgtaagga gcacatgatc cgcagtctta ttggtcagca gccggtcgat
     4621 ggtgacgata ccgtcagcaa atttcgctcc accaaagaga aacacactag gtttttctgg
     4681 cctttgaatc actttttcta gcgaggtgag ctctttttcc ataagtcttc ccgccgccga
     4741 gggaagtaca gcagtaaagc cagtcaggga acattgggcg cggtgcgctg cagcgaatgc
     4801 atcattgaca tagaggtcag caaggggcgc aaggtttttt accaggtctg attgtgcatg
     4861 ctcctctggt tttttctttt cggtctcagc tggatatttt cggacgttat ctaggaagac
     4921 gacggctcct ggcttcataa tctggatcgc atgttttgct ttttcaccgt agatatcatc
     4981 gacaaaagtg accggtttgc caagaagttt ctgtaacgcc tcagcatgtt tctgcattga
     5041 tatgaaatcc cagctccctt gtttcccttg atgggcgagg atcacggttt ttgctttttt
     5101 ttccatcagt tcctgaatgg ttgggagcgc aagtctaatc ctggtgtcat cagtgatatt
     5161 cagagttttt ttatctaagg gcatgttaaa atctacacgg agtagaacgg ttttgttttc
     5221 tacagaaaaa tcatctaacg tgttatattc tttcattacc gaaacctcct ctacggtcct
     5281 atatcaagtg tttttattta aacatatagt tttgaggagt aaaaaggaaa aaaaagatat
     5341 ggttcgactc gaggctcttt agtttttgta ggtttctatc ggaaccatgc gttcattgaa
     5401 gatgtagttg accaaatcaa cgatccggca ggagtatccc cattcgttat cataccagga
     5461 gaatatctta gcgaaattcc ctttttcacc tatcatcttt gtcattaatc catcgatgat
     5521 cgctgagtga ggattgccga cgatatcgac cgacacgagt ggttcttcgg tatattggag
     5581 gatcccttgt aacgtggtct gtgatgcttg tttgaacgcg gcgttgattt cttgtgtggt
     5641 cgtcggtgtt tttagtacgc aggaaaaatc tgttatggag ccatctggtg ttgggacacg
     5701 aagcgcgata ccgtcgattt ttcctttcag atctggtatc actaaagaga ctgcctttgc
     5761 agctcccgtg gtagtcggga taattgacac tgcagcagca cgcgctcttc gtaggtcttt
     5821 atgaggaaaa tcaaggatgc gttgatctcc ggtataggaa tgtactgtgg tcataaaccc
     5881 ttgttctatc ccaaattttt catgtaacac cttcactggt ggggcgagac tgttcgtggt
     5941 gcaagacgcc atggagataa tatgatgttt ggaagggtcg tacatctttt cgttaactcc
     6001 taacactatc gttaggtcag ggtctttccc cggggcggag atgatgactt tttttgctcc
     6061 tgcctccaga tgcttcccag cgccctcacg ggtgcggaaa actccagtgg attcaatgac
     6121 aacatcgatg tctaatttcc cccaggggag atttgtaggg ttgacctcgg agaagaatgc
     6181 aatgagatga ccatttactt cgacgccctc tggttttgct gcaacatcgc cgcagaattt
     6241 accataaata gaatcatatt tgagaagatg tgcaagaatc tccgtgttcc caagatcatt
     6301 aattgcgata acctcaaatt tcttcccgta ctgggagtgt tgtactgcag atcgtagtac
     6361 atttcttcct attcttccaa aaccgttaat agctactcgt atcatctcca aaccacgaat
     6421 ccgacaaaca ggtgaatatt atataagttt tccgacaaga taggttgatg tcgcatgaat
     6481 aatttttact tgaacgaaag atccaatggg aacaggttct gtgagaacaa cttgtttgta
     6541 actgtctgat cttcctgtga cagtcttttt tttcccaggt tcagtgacaa gcaccgaata
     6601 tgtttttccg aggtgttgct tatttttttc aagggagagt tttgcgcaga cctctgtggc
     6661 ttgttttgat cgtgtcttta ccacatgggt aggaattcgt cctttcattt ttttcgcaag
     6721 ggtcaatggg cgagcagaga acctggtgat gttgacgata tctggtctta tttcttttaa
     6781 tagttcaatt gtacggtcga attgttcgtc tgtttctgtg ggaaatccaa tgatcacatc
     6841 ggtcgagagc gtcagagacg ggattgcttc tcggaagtga tggataagag aggtaaactc
     6901 ctttatcgta tacccacgat tcattttttg taaaatttcg tcatcacctg actgtactgg
     6961 gaggtgaagg aatttgtaga tgtttgggtg ctgatatgcc ttcagaacgg aatcaaggtt
     7021 tttttgtaga gttaaaggat tcatcattcc aactcgtatt cggaatgctc catcaagggt
     7081 acttatcctt ttcagtaatg tcccaagatt tgtacccgta tcaagaccat aggacgcagt
     7141 atcctgtgcg gtgagttgta tttctttgca tccttgtttc agagcggaac aaacatctgt
     7201 tacgatttct ccagctgcaa agcttcgtaa tgctccttta gcaaaatgcg tgatacaata
     7261 gctacaagat aatctgcatc cttccgagat cagaatcgga gcaagagccg tatcgtaata
     7321 tttcgggagc gttgtttttt ttgtttcaac aaaaacaggg ttatcaccat taataatatc
     7381 attaatgtac tgtatgtatc gggatgaaag aagcgttgct tgaggtgcta ctgagaggat
     7441 aagatctttt tggacagcag gcatacaacc agtgacgata atcttcttat gggtcttatg
     7501 aaatacttgt aatcgcgata acatccgttg ttcagttgtc gctatgactg tacaggtgag
     7561 cagaaccagc acatctgcac ctgagatgtc cgggactatc tgatgtccct tctgttgcaa
     7621 taggccaaga aggagtcgct catcgctttt gtttgccgta cacccgtacg tctccacata
     7681 gatattcatg ataaaaacgc gaagaggcgc ggtcttattt aactgcttct gtcaccctat
     7741 tcagaacaaa ctgtttcgaa aggtttgata aaaagaagcc agcacgtggt cctcgttcct
     7801 gtccaagaag gattttataa atcgcactaa aagcggtttt tatcgggatt ttcgtttgct
     7861 cagaaatctc atatatcgca ttgtgaattg cctcaggttc ccaggaaaga tccggaatct
     7921 tttccttaaa aatagaaaga atagtccgtt gttcctctgt aagagtgatg gtcggggatg
     7981 tttctttaac ttcgaacttg acctcatcag gtgcaaaggt ggcaagccaa tatcgaacat
     8041 gctcggcccg ctgtctgaga tgttcttcgt cttcttttgt cagtttcttc gtaagctgcc
     8101 cggttcgcat cagtatttct tttacttcag gccatgtttt tgcgatctga tttaatgtca
     8161 ctaaatgccg ataggggaga tgcaacggca tcttttttgg gatatgacac ggctgtgaca
     8221 gctcatagat tttcttgaat tccttcatcc ctttgatttc tgattcttta ccgaaataaa
     8281 ccccttcttc ggtatcgtat tcatcaacta aagtcagcaa tcctaaccca gagtcaaaaa
     8341 caatatgtct gttaggttga tttctcatga tcaagaaccg gagcacctcg ggaggcgtca
     8401 tccgcaacat ttcttcgccg ctcaccgcag tacctttcga gctgtgcatc gcacccatcc
     8461 cctttaacaa gatgaactca tagacaagta cccctggtgg tggatagtga tagacttcct
     8521 ctgatatctt tgctccagtc tcgcgtgtac ctccgacggt cgcatggtct ttgccgcagg
     8581 gttcaaaggt gacccccagc atcctccagc gggctggcca gtccacccgc caagggagtt
     8641 ttccttgtcc tccctttcgg atgtccacct caccgtcatg tccacagtca caggtgtact
     8701 caacgttagg aaattcgtac aaactagggt gtgtcgttga gagacgacca caagtctcac
     8761 atttcacgtt atagggcagc cagtcatttg gcatctttct gccggaaagc gtctcaagga
     8821 tgttcctgat tttggtggtg ttctccaacg cgatctgtat agactcgtta taatcgcctt
     8881 tatagtacat ctcgctagca cggtaaaccc gtgggtttac cccaatagta tgtaatgacc
     8941 caagaaaaga ggtgaggtaa tgatccgcgt agctcgtatg ttcaccacag ggacagggga
     9001 tctcagaaag aggtttaccg acatactgtt gatacgatga ggggagatgg ggatacacct
     9061 ttcgtaacgg atcgtaatca tcgcagatgt agatgaaatc agcatcccca cccttctcta
     9121 tgagacatcg atagacggcg tcagtggtga ggatctcacg catattccca atatgaattg
     9181 gaccagaggg agtaatccca gtcgctagta catgtttcgt ctgctttttc agtagttctt
     9241 ctgctaaaac atctgcccaa tgcataagga aatctccaag ggattatgtg ttagagcgcc
     9301 cgagctcgaa tcaatgagcg gagtggaact ccaccgacat cgttcatcga tcgtttattc
     9361 aacagacaga cgcataaaat cgggtttcct ttttccgctt ttactgcttt gatcgcactt
     9421 tttaaggtct caccggttcc aatcacatca tcgataatca cgacgttttt attcgcgata
     9481 gttgcatagt tagaaccgaa tgcacctgtt ttttcatgat gaggacggaa aacagcaagg
     9541 tcaagaccga gctcctcagc aacataggtc gcaaaaggaa tcccattgat cgcgataccg
     9601 acaaccactt cgacatccac ttcctttttt tccatttctt caaggatgat atcagataag
     9661 gcataggaaa tcgatccgat acgagtcgga gacactccaa tggacctcca cccaactttt
     9721 agttcacctt caggtttctt atccttcccc cgtgtaagaa gccatgctgc tgtttcttta
     9781 gaaacattca gttcctctgc gatctcataa tcggtaagtc cgctctcttt gaattttaag
     9841 gcttttttaa taagagaatc tgtttctttc atatcagaac atcctcaagg ttacgtatca
     9901 tccgacgata tttagatgtt tgctcttttc ttctagaacc attggaaaaa aagcatcgta
     9961 aacctcaaat actgtaagga catgctgtac tacagcgtga ttatatggca caagagaaaa
    10021 aaggtgaagg atttcactct ggcgccgggt taatccggta tttcgatgct gaagaaaaaa
    10081 ccgcatttaa gattccacca tggtttgtgg tgttcgcatg tatagcgacc gctgttgttg
    10141 ttattgcggt ccagctgtgg aaaccgctct aaaaaagaag tttttttatc atttattttt
    10201 ttatctgctg tagtgtgagc tcttttgctt tttccaatgc atcatgtatt ttggttttgt
    10261 ttggcccacc acactgggtg agttttggtt ttccaccacc actccctcca agtatttttc
    10321 cgatttcaga ggcgagtggc cgaaggtcga tgtcaacatt gtccgatgca gcagagacta
    10381 gttttttccc atcatagata tgaacgataa atccagattc tttgatgatg gctcctgcga
    10441 cggtaattgc atcactatcg cagataccac ttaccattcg tatacttgtt cccggaatct
    10501 gagacgcacc ttgaatcaac tgtgcaacga catctgttga aaccattttc ttttgtttct
    10561 gtgcgttctt ccattcattg aacaaatgat tgcaggcatc aacaagactt gccactgttg
    10621 taatattttt tttatttgtc tctattaaaa accgttgtac tgttttttcc agttggtctg
    10681 ctgagactga aaagaatttt tccgtttccc gtaactgctc agcgatgctc tcatgaccag
    10741 tgattttata cgttgatgaa agaagatcag tgacacgatg atacagtttg ttttcttcgt
    10801 tttggtaggc atctgccatt ttactcgcgg caaacgtgat tcgattcaca ccatctgcga
    10861 tgcgttctgc tttcaggatg cggacttttt cgatttcctt ggtgttattc agatgtgttc
    10921 caccacaggc ttccacgtca ataccaggga tgtttagcac ccggatcagg ttcccaggtg
    10981 ggacgcctcc ttggtaaagt ctgaacccat atgctcgctc tgcatcgttc cgtttcatca
    11041 ctttcttttc aacagggact gcttgtcgta tgaactcgtt tgcgattgat tcgattttct
    11101 ggatttcatc ctctgctatc ggcttataat gagcaaaatc aaatcttgcc tcagtcaatg
    11161 ctaattgaga accagcctgc cagatatgtt ccccgaacaa tcttcgcagc gcaccgttga
    11221 ccacgtgggt acccgtgtga tgtttcatga gagtataccg atgatcccag tcgattttcc
    11281 catggacttt tttgcctacc tctgcggggg aatccagtac atggatgata gatcctgctt
    11341 ttttctcaac atggaggact tggatggatt ttccatcgat aacgagttcc cctttatccg
    11401 ctggttggcc acccccctca ggatagaatg ctgttttatc aaggattact tcggttcctc
    11461 gcagggtggt ttgtttccaa aggattgttg catcaaactc tttcgtgtaa tgatcatcgt
    11521 aatagagggg gtgtgtttga ggaagattgg tttgcacttc ttggatttcc tcgtctcctt
    11581 tctcatgaga gtgcaattca gcaatcattg aatcgaaatt aggtgggatg gaaacagaga
    11641 tgttttcttt ttgggcgatg ttctgaacaa cagcaggggg catcccatgc gtatcatata
    11701 gcatgattaa ggcgggggga tcaatggtct gcttttcctt taaaatcctt ttaatgagtc
    11761 cttctccttt tgagagggtt tccgaaaaac gtctcgtctc aatgtctaag atttcgctta
    11821 tctgattctc ccgtttcttt aatgagggaa attctttgcc aaggtaccct ag
//
LOCUS       DATMXJ010000181         7936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_45742, whole genome shotgun sequence.
ACCESSION   DATMXJ010000181 DATMXJ010000000
VERSION     DATMXJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45742"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="glgP"
                     /locus_tag="VN377_04765"
     CDS             <1..1051
                     /gene="glgP"
                     /locus_tag="VN377_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWR27729.1"
                     /translation="YHNGVSKRHGVEARKMWHSLWPEAPEQKVPIDSITNGIHVPTWI
                     EPKMQLLFNKYLGPDWISKHDDPEIWNLIDDIPDEELWQTHYWLKTKLLMRILDRARQ
                     RWMNEQPDPKIILGSGVLLDPNVFTIGFARRFATYKRATLIFQDIERLKNIINDRWKP
                     VQIIFAGKAHPADDQGKQLIQQVFNIAKDTSFGGRIAFVEDYDENLAQYLTHGVDIWL
                     NNPQPPYEACGTSGMKASLNGVPNLSIFDGWWIEGYNGKNGWAFGDKVGNRSDSEDAA
                     DIYQLLEKKIIPLFYDVNEDGLSIKWIKLMKEAIKSTARPFSARRMMKEYTQKFYNPA
                     FDFASRTQNIKKDVS"
     gene            complement(1061..2536)
                     /gene="pyk"
                     /locus_tag="VN377_04770"
     CDS             complement(1061..2536)
                     /gene="pyk"
                     /locus_tag="VN377_04770"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWR27730.1"
                     /translation="MVDTKAKQKIKLPSHKTKIICTIGPASRPPEVLEELMQNGMNVA
                     RLNFSFGNSTEHRKDIRLIKKTAAELEKPITILIDLPGSKIRIGTLESEPIILKKNSQ
                     ITLTSQDVHGTDTLIPVKYSQLLKSVSKGQTIYFNDGFIQLKVKAVVNNEVQCNVIIG
                     GALYSSKGVNLPGSRLFLDAVTQKDLDYIDFGLKERVDTFGVSFIQNANDILQVKEYA
                     RKKGKTIYTVAKIERKDAVKNFEQILLVSDSVMIARGDLGLEIPLEDVPSVQKKLINK
                     ANLAGCPVITATQMLESMKENTRPTRAEVADVANAILDGTDAIMLSEETAIGKYPIET
                     VQMMRKIAKATELKRRDAPSSCNLQDILRRTVSIDHNSVTDIISLNVVEAAETLQVRY
                     ILTPTASGNTARRISRFKPECWILAFSGQKDTCPFLNLSYGVYPFFMTKKTKSWHAPI
                     LNFINDVGLVKKNDKVILTQRRFSKKPGGTDSFGILTIGAE"
     gene            2768..3586
                     /locus_tag="VN377_04775"
     CDS             2768..3586
                     /locus_tag="VN377_04775"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HWR27731.1"
                     /translation="MGKFGRSWQLMKTSLGILRKDKEIMIFPILSFIACIIILLSFFA
                     GFWLLGLPSMDTLPWLWVVAMFLIYFVLFFIVIFFNTAIIACANIRLNGGDPTVSDGL
                     RIASQNIGRILIWALISATIGMILQAIREKGGWIGRIIAGVFGIAWTYVTFFIIPVLI
                     YEKKGVASSIRRSASLFKQTWGETIIGTIGFGVVFFVLALLGIVPIILGIYLGSTISI
                     AVGFILAFFYWAIIGVVASATNGIYVAAMYHYATKKQLPPEFDSSLVPPTIQGF"
     gene            complement(3610..4599)
                     /locus_tag="VN377_04780"
     CDS             complement(3610..4599)
                     /locus_tag="VN377_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HWR27732.1"
                     /translation="MNSSHFVKILREKKLFLPPLSGYTDYPFRVILAKFQPPFIITEM
                     ANARAIVQKNRRTMQILRIVEGNHFNGVQLVGSIPEYMRKAAEIVQDLDFDYIDINMG
                     CTARKVTCRGEGVSLMKNEDNACQIVAAVGRAVDVPVTCKMRLGVSKQSMNVLSLSQK
                     LVDAGATALTIHGRSGEKKFGVHLDRDIIKKTATILSVPVIANGSIYTGFDAQKMIQE
                     TGAAAVMPGRGLLGNPWIIPEILNILSHQKYTPPPLQQKKEVCLKHLDLLVDFYGERR
                     AVLKMRSILPHYFSSCLFLKELKKDVQQITTAREISLLLERIQDNEMNAVYQR"
     gene            complement(4653..5867)
                     /locus_tag="VN377_04785"
     CDS             complement(4653..5867)
                     /locus_tag="VN377_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27733.1"
                     /translation="MFRDHVKLAIKNLRNRMSRSLLTLLGIAIGIMAIISLLALGEGM
                     QQAVTGELSSLSDVIIVSTGGGGFSSFGGGGSTVDYFTQRDIAEVERLQGVREVSTQL
                     SGTGLAEYNGKTTILSLTGMDVSVMKLQYAGQNLDAGEFLNEGDQKKIMIGYNIAHDT
                     FDADIAVGGRIKINGEKFFVSGIFGKQGFGGVSSDSIVLMSSRDFQKLTGQSNISIIY
                     LRVYNVNDAESIATTIQNAINENHGKKDFATATTMTSILKTVQNIIGILQLVLVGIAS
                     IALVVASIGIMNTMLTSVMERTREIGIMKAIGATTRDIMSIFIIEGMLMSSLGGIIGI
                     ILGVFGSQALTMILKSFMSMGGGSFQLAPIITITSVILAVGVSLIVGVFSSLYPAWKA
                     AKMSPIEAVRYE"
     gene            complement(5860..6543)
                     /locus_tag="VN377_04790"
     CDS             complement(5860..6543)
                     /locus_tag="VN377_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27734.1"
                     /translation="MSKDVIVTQNVSKTYGKPPLQVFALRETNLQIEQGDYIAIIGPS
                     GSGKSTLMNLLGCLDKPTTGNIFIDGKDVSTLNENELARIRREKIGFIFQKYNLIPTL
                     NALENVELSMGFAGVDSQTRTAKAKKLLELVELSKRLTHKPSEMSGGEQQRVAIARAL
                     ANNPSIILADEPTGNVDTKSGENIMNILEEVNRKGETIIVVTHNMTIAQRAKRVLRIQ
                     DGVVKEEHV"
     gene            complement(6543..7838)
                     /locus_tag="VN377_04795"
     CDS             complement(6543..7838)
                     /locus_tag="VN377_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27735.1"
                     /translation="MKKIMTTTIIITMIISALVSLYLPTVQASPAVMISQYQLSPEIL
                     MPGDTAVLTLQVRNAETVATQTTISGTSPYDSTTTVRTLGVDINNIWIVAAQSGGKQI
                     RATANYEDVGYLAAGATIEVNFKLIVDKNMSEGIYFPIVNIDVSSGTDVIYPLLVKVS
                     NASVDLLPTSVPSKISKGGTTELTVTAVNERENAVKEVIITPHGDGVEFVPQSYFIGS
                     LAAETSEDVVFSVKPIETRESNISFSVSYKNGDNLHTNSLVAPLSVIEALDVAPIITN
                     FPLSITKGGSARISIEVYNAKTEKITGVLVTPICNATVIPSQYFIGSMDPDDVFSASF
                     DIYADTVGYGTQIIGFKVSFKQGNDYYETPLVSKTFSVVSGPGQSYQSSGSSTGTSGS
                     NGTPQTPSLVTCLTSIILIIVVIVVVVLLYIRWKKRRKA"
BASE COUNT         2188 a         1722 c         1613 g         2413 t
ORIGIN      
        1 gtatcataat ggagtcagta aacgccatgg ggtagaagct cgaaagatgt ggcattcgct
       61 gtggccagag gcacctgaac aaaaagtccc tattgattca attactaatg gtatccatgt
      121 gccaacgtgg atcgaaccaa aaatgcagct tcttttcaat aaatatcttg gaccagattg
      181 gatctcaaag catgacgacc ctgagatttg gaacctcatc gatgatatac cagatgagga
      241 actctggcag acccattatt ggctcaaaac aaaattgttg atgcgcatct tggatcgtgc
      301 acgtcaacga tggatgaacg aacaaccgga ccctaagatt attctcggat ctggcgtatt
      361 gcttgatcca aatgttttta ctataggttt tgcccgtaga tttgcaacct ataaacgagc
      421 gaccttgatt ttccaggata tcgaacgtct taaaaacatt ataaatgatc gctggaaacc
      481 cgttcaaatc attttcgctg gaaaggcgca tccagcggat gaccaaggaa aacaactcat
      541 acaacaggtg tttaacatcg caaaagatac ttcctttggt gggcgaattg catttgttga
      601 agattacgac gaaaacttag cccaatattt aacacatggt gttgatatct ggttgaataa
      661 tccccaaccg ccctatgagg cttgcggaac gagtgggatg aaagcctcat taaatggcgt
      721 cccgaatcta agtatttttg atggatggtg gattgaggga tataacggta aaaacggatg
      781 ggcatttggg gacaaagtgg gaaatcgaag tgacagtgaa gatgctgcgg acatatacca
      841 acttcttgaa aagaaaatca taccactctt ctacgatgtc aatgaagacg gattatctat
      901 taaatggata aaacttatga aggaagcgat caaaagtact gccagaccct ttagcgcacg
      961 cagaatgatg aaagaataca cacagaagtt ttacaaccct gcatttgatt ttgcatccag
     1021 aactcaaaat attaaaaaag acgtatcatg aaaaacaata ttattcagcc ccaatagtaa
     1081 ggattccaaa tgagtctgtc ccgcctggtt tctttgaaaa ccttcgttga gtgagaatca
     1141 ccttatcatt ctttttaaca agaccaacat catttatgaa gttaaggatg ggagcatgcc
     1201 agctcttcgt cttttttgtc ataaaaaaag gatatacacc atatgagaga ttgagaaacg
     1261 gacaggtgtc tttctgtccg ctaaacgcaa gtatccagca ttcgggttta aatcgtgaaa
     1321 tacgtcgagc tgtatttcca cttgcagtcg gcgtgagaat ataccgtact tgtaaggttt
     1381 ccgcagcttc cacaacgttc agtgatataa tatctgtaac gctgttatga tcaatgctta
     1441 cggtacgtct caagatatcc tggagattac atgatgatgg agcatctctc cgtttcagtt
     1501 ccgttgcttt agcgattttc ctcatcatct gaaccgtttc aatcgggtat tttccaatcg
     1561 cggtttcttc agagagcatg atcgcatcag taccgtcaag aatagcgttt gcgacatctg
     1621 ctacttccgc tcgtgtcgga cgggtgtttt ctttcatcga ctctaacatt tgtgtcgcgg
     1681 taatgaccgg acaacctgcg agattcgctt tgtttataag ttttttttgc actgatggaa
     1741 cgtcttctaa gggtatttca agaccaaggt ccccccgagc gatcataacc gaatctgaga
     1801 caaggagaat ttgttcaaaa ttttttactg catcttttcg ttcgattttc gctaccgtat
     1861 atattgtctt tccttttttc cgagcatatt cttttacctg aaggatgtca tttgcatttt
     1921 ggatgaaaga aacaccgaac gtgtcaaccc tttcttttaa cccaaaatca atgtagtcaa
     1981 gatctttttg tgttactgca tctaggaaca atcttgaacc tggcagatta actcccttcg
     2041 acgaatataa cgctccaccg ataattacat tacattggac ttcattgttt accacagcct
     2101 tgacttttaa ttggataaat ccatcgttga aataaattgt ttgtcctttg gaaacacttt
     2161 ttagtaattg actgtatttc actggaatga gcgtatcagt cccatgaaca tcctgtgaag
     2221 tgagtgtaat ttgagaattt ttcttaagaa tgataggttc ggattctaac gttccaattc
     2281 tgatttttga accgggaaga tcgataagga ttgtaattgg tttctctaac tcagcagctg
     2341 ttttctttat cagtcgaata tcttttctat gttcagtaga attaccaaaa gaaaaattga
     2401 gccgggcgac attcattcca ttttgcatta attcttctaa aacctcagga ggtcgagatg
     2461 caggaccgat tgtacatatg atttttgttt tatgagatgg caattttatt ttctgctttg
     2521 ctttcgtatc aaccatgtaa ttcactattt taggaaatga tacgtgtgtt tcgtttaaat
     2581 attatcctct gcatattctc agatattggg ttgtgcatcg attcatggaa tgccctcgga
     2641 attattaagg tcggatggaa agttaccatt ttcctaatct gctctggatg actaacaaaa
     2701 aatacacatt atctgccctc caccgcatga aaatctttaa ttatctgcta ccttcttacg
     2761 ggtcttcatg ggaaaatttg gacgtagttg gcaattaatg aaaacaagcc ttggtattct
     2821 ccgaaaagac aaagaaatca tgatcttccc catcctttcc tttattgcat gcattatcat
     2881 tcttctttca ttcttcgcag gattctggct cttaggatta ccttccatgg ataccttacc
     2941 atggctctgg gttgttgcga tgttccttat ttactttgtt ttatttttta tcgtcatttt
     3001 ctttaatact gcgatcatcg cctgtgccaa catcaggtta aatggtggcg acccgaccgt
     3061 ctctgatgga ttgcgtatcg cttctcaaaa tatcggacga atccttatct gggcgcttat
     3121 atcggcaacc atcggaatga tcctacaggc aatccgtgag aaaggaggat ggatcgggag
     3181 aatcatcgca ggtgtttttg gtatcgcttg gacgtatgtc acattcttta ttattccagt
     3241 cctcatctat gagaaaaaag gggtcgcgtc ttctattcga cgtagtgcat cgttgttcaa
     3301 acaaacatgg ggtgaaacca tcatcggaac tattggattc ggggtcgtct ttttcgtact
     3361 agcgcttctt gggattgtcc ctatcatcct tggtatatac ctcgggagca ccatcagtat
     3421 cgcggtcggt tttattcttg catttttcta ctgggcgatt attggggtgg tcgcatcagc
     3481 gaccaacggg atttatgtcg cagcaatgta tcactatgca acgaaaaagc aactccctcc
     3541 agagtttgac tcatcgctgg taccaccgac aatccaagga ttctaagtaa aagaatttct
     3601 ccgatatatc tacctttgat aaactgcgtt catttcatta tcttggattc gttctaataa
     3661 gagcgagatt tctcttgcag tggttatctg ttgaacatcc ttctttaact ccttgagaaa
     3721 caaacaagag gaaaaataat gcggtaagat acttcgcatt ttcagaactg cacgacgctc
     3781 accataaaaa tcaacgagca aatctaggtg tttgagacaa acctctttct tctgctggag
     3841 tggaggcggt gtatacttct ggtgcgataa gatattcaat atttcaggta ttatccaggg
     3901 atttccgaga agaccccggc ctggcatcac tgcagctgca ccagtttcct gaatcatctt
     3961 ttgggcatca aatccagtgt aaatacttcc atttgcaatt acagggactg aaagaatcgt
     4021 cgctgttttt tttatgatgt cccgatcaag atgtactcca aatttttttt ccccacttcg
     4081 cccatgaata gtcagagcag tcgctccagc atcaactaat ttctgtgaaa gagacaaaac
     4141 attcattgat tgtttcgaga cgccaagacg catcttgcag gtcaccggaa catccacggc
     4201 tcttccaact gcagcaacaa tctgacaggc gttatcctca tttttcatta gagatacgcc
     4261 ttcacctcga caggtgacct tccgtgctgt acaccccata ttaatatcga tgtaatcaaa
     4321 atcaaggtcc tggacgattt ccgctgcttt cctcatatat tccgggatgc ttccaacaag
     4381 ctggactccg ttaaagtgat tcccctcaac aatcctcagt atctgcatgg tccgtcgatt
     4441 tttctgtacg atcgcacgag cattcgccat ttctgtaatg atgaatggtg gttgaaactt
     4501 ggcaagaatc acgcgaaacg gataatcagt atatccactc aatggcggca aaaataattt
     4561 tttttcgcgg agaattttca cgaagtgcga ggagttcata aataaggagg catagaatag
     4621 gagatattaa tgtatcttgt tgtctaaaaa agttattcgt atcgcacagc ttcgatcgga
     4681 ctcatctttg cagccttcca tgcaggatag agacttgaga aaacaccaac gatgagtgat
     4741 actccaacgg caagaatcac agacgtaatc gtaataatcg gagcaagttg gaacgagcct
     4801 cctcccatcg acataaaact cttaagaatc atggttaacg cctgcgatcc aaacacacca
     4861 agaataatac caataatccc accaagacta ctcatcaaca taccttcgat gatgaagata
     4921 ctcatgatat cgcgagttgt cgcacctatc gctttcataa taccgatctc acgagttcgc
     4981 tccataaccg acgtaagcat cgtattcata atcccaatcg aggcaacaac aagtgcaatc
     5041 gacgcaatgc ccacaagaac taactgaaga ataccaatga tattttgcac tgttttaagt
     5101 atagaagtca ttgtcgttgc agtcgcaaaa tccttcttcc catgattttc gttgatagca
     5161 ttctgaatag tggtcgctat ggattctgcg tcattgacgt tgtacacccg taaatagatg
     5221 atactgatgt tactctgacc ggttaatttc tggaaatcac gggaactcat caacacaata
     5281 ctgtctgagg atacgccacc aaaaccctgt tttccgaaaa taccgctgac aaaaaacttc
     5341 tctccattaa tcttgattct cccaccaaca gcaatatcag catcaaaggt atcatgtgcg
     5401 atattatatc caatcatgat ctttttctgg tcaccttcgt tgagaaattc tcctgcgtcc
     5461 aagttctggc ctgcatattg tagcttcata acgctaacgt ccatgccggt cagtgagaga
     5521 atcgttgttt tcccgttata ttctgcaaga ccagtaccag acaactgggt gctcacctct
     5581 cgcactcctt gaagccgctc gacttctgcg atatctcgct gagtgaaata atcaacggtt
     5641 gaacctccac caccaaacga ggagaaacca cccccgccgg tagacactat gatgacatca
     5701 gataatgaag agagctcccc ggtgaccgcc tgttgcattc cttccccaag agccaataga
     5761 gagataatcg ccatgatacc aatcgcgata ccaagcaatg tcaaaagtga tcgagacatg
     5821 cgatttcgaa gattttttat cgcaagtttg acatgatctc taaacatgtt cctcctttac
     5881 cacgccatct tgaattcgaa gcactcgttt cgcgcgttgc gcaatcgtca tattatgggt
     5941 tacgacgatg attgtttcac cttttcggtt gacttcttct aaaatattca ttatgttttc
     6001 gccggatttt gtatcaacgt tcccagttgg ttcatctgca agaataatcg aaggattgtt
     6061 cgctaatgca cgagcaattg caacccgttg ttgttcacca ccactcattt ccgatggttt
     6121 atgggtcaat cgttttgaaa gctcaaccag ttctagaagt ttctttgctt tcgcggttcg
     6181 agtctggcta tcaactcctg caaatcccat tgagagttcc acattctcca aggcatttag
     6241 tgtcggaatg agattgtatt tctggaagat aaaaccgatc ttttcacgtc gaatcctggc
     6301 aagttcattt tcatttaatg ttgagacatc tttcccatcg atgaagatgt tcccagttgt
     6361 cggtttgtca agacaaccaa ggagattcat cagcgttgat ttccctgaac ctgacggacc
     6421 gatgatggcg atgtaatctc cctgttcgat ttgaagattt gtctcccgca atgcaaagac
     6481 ctgcaagggt ggtttgccat aggtttttga gacattctgt gtgacgatga catccttact
     6541 cattatgcct tccgccgttt tttccaccgt atatagagca gtacaaccac gacgattacg
     6601 acaatgataa gaataattga tgttaaacat gtcactaacg agggagtttg aggtgtacca
     6661 tttgacccag aagtgcctgt ggagctacca gaggattgat aactctgacc gggtccagag
     6721 accacgctga atgttttact taccaatgga gtctcataat agtcattccc ctgtttgaag
     6781 gatactttaa atccgatgat ctgcgttccg tatccgactg tgtcagcata gatgtcaaac
     6841 gacgcggaaa atacatcatc aggatccata gagccgataa aatattgcga gggaatcacc
     6901 gtcgcattac agataggggt tacgagaaca ccggtgatct tctctgtttt tgcgttgtac
     6961 acctcaatgc ttattcttgc agagcctcct ttcgtgatgc tgaggggaaa attggtaatg
     7021 attggtgcaa catctaacgc ctcaataacg gagagtggag cgacgagtga gtttgtatgt
     7081 agattatctc catttttata gctcacagaa aaggaaatat tactctccct agtttcgatt
     7141 ggcttgactg aaaaaacaac gtcttctgat gtttctgcag caagtgaccc tatgaaataa
     7201 ctctgtggta cgaactcgac accatctccg tggggagtga taatgacttc ttttacagcg
     7261 ttttctcttt cattcaccgc agtaacggta agctctgttg tcccgccttt ggagattttc
     7321 gaaggcacac tggttgggag gaggtctact gaagcattac ttactttgac taacagagga
     7381 tagatgacat ccgttccgct gctgacatcg atattcacaa tgggaaaata aattccctca
     7441 ctcatgtttt tgtcgacaat gagtttgaag ttcacttcga tggttgctcc tgctgcaaga
     7501 tagccgacat cttcatagtt tgctgtcgcc cggatttgtt tacccccaga ttgtgctgcg
     7561 acaatccaga tattattgat atctacgcca agtgtacgaa ctgtggtcgt tgaatcatag
     7621 ggggaggtac cagatattgt tgtttgtgtc gcgacggttt ccgcgtttct cacctggagg
     7681 gttaacaccg cggtatcacc aggcatgagg atttctgggg ataattggta ttgtgagatc
     7741 attacagctg gacttgcctg tacggtggga agatacaggg atactaatgc actgataatc
     7801 attgttatta tgatggttgt tgtcattatt tttttcatat taatttacct ccttttgtaa
     7861 atcagattcg caacgtctga gtatttttat tacttccttt tgtttttctt tggataatgt
     7921 taagacggat gtaaat
//
LOCUS       DATMXJ010000182         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_56095, whole genome shotgun sequence.
ACCESSION   DATMXJ010000182 DATMXJ010000000
VERSION     DATMXJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56095"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..798)
                     /locus_tag="VN377_04800"
     CDS             complement(337..798)
                     /locus_tag="VN377_04800"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWR27736.1"
                     /translation="MNVCLGGTFHPFHKGHKTLLRKAFEVAGPKGSVFIGLTSPTMIR
                     KKGKIASFQKRKQSIVRFLSEEHVLQQATIRPLVNEFGPTLEEDFDAIIVSPETEPTA
                     EEINRKRVRLGKTPLQIIVIPYALSEDGTPISSSRIRRKEIDENGRILKQD"
     gene            894..3005
                     /locus_tag="VN377_04805"
     CDS             894..3005
                     /locus_tag="VN377_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27737.1"
                     /translation="MSTKRSIFLVVSLVFLLLNGLSLLFLTEYSNAAGREIYVDDSFS
                     YPRDGSAEHPYQTITEAIKLANDGDTVYVFGGTYNESLVINKRISLIGGIDDQQSFIS
                     RGVELKYLIEITADFVTVENFVISDPGRFISSQYGALIHVTSNNVVLQKNNISQCDLW
                     GIYLDSSDDNMISGNLINDTKGVFVSSSNNNVFSSNNISNSSDSGMNLRSSIRNIFYD
                     NNLTHNTYGIYTRECSNTNITKNTFAKNTYHGIYLTGDTTDVVRENYFNNNTVSAITV
                     DSSGCIIAENLFNYGQVGISLQRIDCQIFNNSLQNMSSTALVATQTSKNNIIYLNHFL
                     ENNINAREQGSNQWDNGTIGNYWDDYNYVDRNHDGIGDRPYIISTGGRDHYPFGMFLQ
                     PPQKPSNPSPADDQEGVGLSVTLWVTVMDPDSNVISDVSFYNAVNNVRIGDVRNVVNG
                     KNASCSFTLPFDTTYAWYVIANDSLQQNQSDIWFFTTKQRPPENQKPVANPGGPYVTK
                     LNQSISFNGSQSIDPDGSIIFYRWNFGDGSSQILDISPEHTYADPGVYTVTLTVVDDD
                     GRSSMANTTATIQGVIFINDPPVATFIAPSSVSVGQEVSFDASLSNDTDGTIVGYRWD
                     FNGDGTYDTDWVTTPVMTTMFSTAGSFVVTLEVIDNNDAVGPYSTTVSVKESKKNTPG
                     FDIVLVLVALLIGFFMYKKYV"
BASE COUNT          855 a          584 c          642 g          930 t
ORIGIN      
        1 accattaact ccaaaattta ttcaagggac ggttcgcact cgttttcatt attttgaccc
       61 ttttgaccaa acaaccgttt atatatatcg gataggtttt ttaattgatt ttttcccaaa
      121 gttattgcca atctcttaag aaaatgatat tttaagataa agaataagaa accatctgaa
      181 attgtcgaat gatggtgtag taatcctgtt tttactcaag cataagatat atgaagcgct
      241 tgcctgattg tttatatggt gataactgaa atatgaaagg agaaaacgaa ccaggagaaa
      301 atatttgtat ttcaggaaac tttattctat ataaatctaa tcctgcttta gaatccttcc
      361 gttttcgtcg atttccttcc gtcggattct tgacgagcta atcggggttc catcctctga
      421 aagggcatag ggaatcacga tgatctgcag aggtgttttt ccaagacgca ctcgtttccg
      481 gttgatttct tcggcggtcg gttctgtttc aggggaaacg atgatggcat caaagtcttc
      541 ctctagcgtg ggaccaaatt cattgacgag aggtcgaata gttgcctgtt ggaggacatg
      601 ttcttctgat aagaaccgca caatcgattg ttttctcttc tggaatgaag caatttttcc
      661 tttctttctt atcattgttg gtgacgtcaa cccaataaac acagagcctt tcggtccagc
      721 gacttcaaac gcttttctga gaagggtttt atggccttta tggaaaggat gaaacgtccc
      781 accaagacag acattcatgc aagtaacaat acttggagat tctttaaatg tatgcgttcg
      841 gaaaactatt taattctgtg acatgattcg tctttcatta aatggatggg ataatgagca
      901 caaaacgttc gatttttctt gtggtaagcc tcgttttttt attattaaac ggtctttcgt
      961 tattgtttct tactgaatat tcgaatgcag caggaagaga aatttatgtc gatgatagtt
     1021 tctcataccc gcgggatggg tccgccgaac atccgtatca gacaataact gaggcgataa
     1081 agcttgcaaa tgatggtgac accgtgtatg tttttggtgg gacgtacaat gaaagtttgg
     1141 tcatcaataa gcgtatttcc cttatcgggg gaattgatga tcaacaaagt tttatctctc
     1201 gcggtgtgga actaaaatac cttatcgaga tcactgctga tttcgtcact gtagaaaatt
     1261 tcgttataag cgacccggga cgttttattt catcccaata tggtgcgctt attcatgtga
     1321 cgtcaaacaa tgttgtattg caaaagaata atatttcaca atgtgacctc tggggaattt
     1381 atcttgattc ctctgatgac aatatgatta gtggcaattt aatcaacgac actaaaggag
     1441 tgtttgtttc ctcctccaac aataatgtat tttcgagtaa taacatctcc aattcaagtg
     1501 attcagggat gaacctgcgt tcttcaatta gaaatatttt ctatgataac aatcttacac
     1561 acaacacgta tggtatttac acacgagagt gttcgaatac gaatattacc aaaaacacgt
     1621 ttgcgaaaaa cacgtatcat ggtatctatt tgacagggga tacaactgat gttgtgcggg
     1681 aaaattattt taacaacaac accgtgagtg caatcaccgt tgattcttcc ggttgcatca
     1741 ttgcggaaaa tctgttcaac tatggtcagg ttggtatcag cctgcaaaga atcgactgtc
     1801 agatctttaa taactcactg caaaacatga gctccaccgc tcttgttgcg actcagacga
     1861 gtaaaaataa catcatatac ttgaatcatt ttctagaaaa taatattaat gcgagggaac
     1921 aagggagtaa tcaatgggat aacgggacga tcggaaatta ttgggatgat tataactatg
     1981 ttgataggaa tcatgatggc atcggtgaca gaccctatat aatttcgacc ggtggtcgtg
     2041 accattatcc ctttggtatg ttcttgcaac caccacaaaa accatcgaat ccatcgcctg
     2101 cagatgacca agagggtgtc gggttgagcg tgacgctgtg ggtaacagta atggatcccg
     2161 acagtaatgt catctcagac gtctcctttt ataacgcagt aaataacgtg cgaatagggg
     2221 acgttcggaa cgtggtgaat ggaaagaatg cctcttgtag tttcacattg ccttttgata
     2281 caacgtacgc ctggtacgtc atcgcgaatg atagtttgca acaaaaccag tctgatattt
     2341 ggttttttac gacaaaacaa cgtcctcctg agaatcaaaa accagtggcg aatcccgggg
     2401 gaccctacgt aacgaaactg aatcagtcga tctcctttaa tgggtctcag agcatcgatc
     2461 ctgatggatc cattatcttt taccggtgga attttggtga tggtagcagt caaattcttg
     2521 acatatcacc agagcatacc tacgctgatc ctggtgttta taccgtgacg ttaaccgttg
     2581 ttgacgatga cgggcgtagt agtatggcga atacgacagc gaccatacaa ggcgtcattt
     2641 tcatcaatga ccctccggta gctaccttta tcgctccctc ttcggtttcc gttggtcaag
     2701 aagtgagttt cgatgcctcc ttaagtaacg ataccgatgg gaccattgtt gggtaccgtt
     2761 gggattttaa cggtgatggg acttatgata cagactgggt aaccaccccg gttatgacca
     2821 cgatgttttc tactgcaggc tcctttgttg tgacgttgga agttattgat aataatgatg
     2881 ccgtgggtcc ctattcaacc acggtatctg tcaaggagag taagaagaac acacctggtt
     2941 ttgatatcgt tcttgtcttg gttgcactcc ttatcggttt cttcatgtat aaaaaatatg
     3001 tgtaatacga t
//
LOCUS       DATMXJ010000183         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_40522, whole genome shotgun sequence.
ACCESSION   DATMXJ010000183 DATMXJ010000000
VERSION     DATMXJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40522"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN377_04810"
     CDS             <1..272
                     /locus_tag="VN377_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27738.1"
                     /translation="NIHVEKAPGETYDVSPFDVILISYGVGDIEAVLKNTLQHLKNNG
                     KVILRKPITETTEYIDTVLEDYSIKKIKLLLSQESYLLMKAQSAS"
     gene            complement(269..1588)
                     /locus_tag="VN377_04815"
     CDS             complement(269..1588)
                     /locus_tag="VN377_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27739.1"
                     /translation="MTIDDPKNPWPPEEEGDHDPVMREWWTSELLFQTREDHRRWNLM
                     TSFAYEQETPSCFFQYVLFDITSKKWVVHKDLNDEITKLAHVKNRLGLRYEASHLEGL
                     YPKYHLHVEDPKQGFILDADYTARSLPHWIAQEATQGNLPIGLNFYRYGFLPHCDIIG
                     TMILGKESYSIEGKGYLEHAYGNWSYQNPLQTISRAHRTLTTYGHLIRWWLSHHRFKI
                     PKRISFSSENNPFGYDWVWGVFDNSWSLFFGNSLFWVSEGPSIGALYLTADGKRYWEF
                     CDVRFKYNKVLFVKEYDLYYPSDLEVTGRLEDKTIRLRFWSTTDTYEYIDPYRKGRFY
                     KAFILSELPGRMEGTFTDKERTVPLQGECKIVPLRQAPLLGHNAVSFEFLTPPKGVGL
                     SVELDSHALRKQLITKIQLAPRPKISCHLKRIDLSQIPKESEVRRES"
     gene            1756..2718
                     /locus_tag="VN377_04820"
     CDS             1756..2718
                     /locus_tag="VN377_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR27740.1"
                     /translation="MLQKLFAHLETMRPYTLLWCGLVSLLGASLRYGDIPPIQISLLV
                     FFIPILGWIAGLYLADYYDKGLDAIQKPHRPIPSGRIVPREALIVGASYAILGLGLTL
                     FLPLINILLIFIAGALVLCYAKYTKAKGLLGNFNRGAMTVITFLFGVFSIDMTTAIPL
                     SLWLLSLVFFFHDTNSNIIGAIRDIHGDKTGGYATTPVKYGIRLTLRISVLLSIVYLL
                     LTAGIISFYPLILYPTYLLMLFVIGILILCVMYMILFTSVNTLTQKQSLYAHELFVAE
                     RIIFSSAFIVGIASSHMLSFTLCIVSLVTTLLSQHLLRERYELT"
     gene            2724..3662
                     /locus_tag="VN377_04825"
     CDS             2724..3662
                     /locus_tag="VN377_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWR27741.1"
                     /translation="MNCLVTGASGFIGFALVKRLVQDGHHVRAILHKTSPPHRETQVD
                     YIIADLTDPVSLTPLVHDVDIVFHCAAHVKDFGSKKEIMDVNLKGTQHLVKACGNTIR
                     QFIFLSHLHGTSPTYIGPYSSSKGLAEQYLLQQHQNNQFPVVIIRPGSVFGPGATTWC
                     LRPLQAIQQDRIALIDHGTGIFLHTYIENLLDGLLSIPTTSHLEGEIIEITDGDNTTT
                     WKTYLNDLAALAGKQPITRNISKTTASLISHSMMIRYYLFHKTPLLTPTAVHLFTNHR
                     TVSIEKAHKLLGYSPMVDYPEGMKRIAQWLQKEQYI"
     gene            complement(3689..5008)
                     /locus_tag="VN377_04830"
     CDS             complement(3689..5008)
                     /locus_tag="VN377_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27742.1"
                     /translation="MNILLVFPRIEHGATTYSDKGSWSSILFGYPIITLPHLAGITPR
                     QHTVKIVNENYEDIDFDEPTDLVGITSYTMTAPRVYEIADEFRRRGKKVVLGGYHPTA
                     MPQEAIQHADSVVLGEAELTWPELIHDVEKGVLKQFYGPNPDFDMAEIPPIRRDLIRQ
                     NPMIGAVQSTRGCPNQCEFCAIASFCNHAVKHRPVKNVVDEITQMPNRIFVIHDPSLT
                     VNPSYSRELFKELIRQKVHKGWVSNGNSNVLGKIDDEFLDLAKKSGCVEWFVGFESVS
                     QEALNGIKKTVNKVEDFKKTIKRLHKHGMAVQGGIIFGFDQDTPEIFDLTFEKMNEWE
                     LDAVEINILTPFPGTPLYDRLEREGRIISKDWKRYNQVDVVFKPKHMTEKELFEGARK
                     VAKKYYSMPNVIMRALRTFAIVRNLPAVLPAGTNYTFRRYYKRDFNF"
     gene            complement(5092..7230)
                     /locus_tag="VN377_04835"
     CDS             complement(5092..7230)
                     /locus_tag="VN377_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWR27743.1"
                     /translation="MKLVPPDQGMLYYIIENKRPDLAKKIKQSGVIETIVVGLGGQGT
                     RHAELMQQYGTTITAGIAPGRGGTRLLETIPVYDTISECLKEHPNIAVASVWRHYSTA
                     KDATIEAIEAGIPLVILISEGIPLKDVRDILVAARKNKTVLIGGNTPGVIFPPEGIKA
                     GMLPDVFYPEEVSPEEFGPKGVTIISRSGAILYHLSDALASVGIAQNAVLGVGGDGAI
                     GSTFRDLVPLAMAYKNTDLVVVAGEIGGCQEELLADDIKKNPKKYPKPLVALISGAHA
                     PEGKTMGHAGAIVSPGQVYGTFQSKKQALESVGIPVVNSQYDLITEVQKRLQKKIYFD
                     VENYYKKMKTIWDAPSKKRGWGTLITKVMPNNLLISGYPLQDIVEQKGFLETAYLLVK
                     GEFPDKKTYEEMRKIAVEAAKKPVPKVSRSKKEDISKTLVKYFVLDEELAQYPEEGTN
                     GAVKKTIFGIGRTARYLSGILGTEEALEKLSGNEPFSHVVYCAVTGNTAVNEQHSRMI
                     EAMIVACVDHGVTPPSAQATLIAASTRAPYEVAVAQGVGAITDVHGGAGAKAAQLFIE
                     CFMKSKKENIDVAQATREIMRKYIEEGKRIEGLGHRLHTKDPRRDVLWNLSSQTGIAG
                     EHVQLSKKVSTIFEQVRGMSLPINVDGVIGAIVADMKLDPAMAKAFFIYGRIAGLSAH
                     YFEEVASQPRMRQINFTEAVYIGKEPRTIP"
     gene            complement(7227..8510)
                     /locus_tag="VN377_04840"
     CDS             complement(7227..8510)
                     /locus_tag="VN377_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWR27744.1"
                     /translation="MKLYEFEAKKVFEKMGIPIPKQYGVIRSLKELDTLKLEFPIMLK
                     SMVLVGGRGKAGGIKKANNVEEARKIAAVLFTLKIKGFPVDCILLEEAVEEIGACYVG
                     ITMDPATFNNVVIVSASGGVDIEQVAMKNPQAIVRKEIPDNNKEISKTIAQDLASSLN
                     QFLKGKNESTKILAEIIAKVYATYQRFDARLCEINPLIITSNGAIAADAKLVLDDNAL
                     FRQVELLEMLGITEKRHDVAERTKNELRAQSAGFPYIDLLPQPFTKDQKKLYVGLIPG
                     GAGYGIFSIDEVSTIGNRYFHDRVVPVNFMDSGGGPSQNRVAEMFHLLMDYPVVDLIV
                     TSRFGGISSCDIFIRGMVQAVKERYESGKRMVPVFGRMVGTDLPSARAFLEKAKAESS
                     EALQNVTIYVGNQRIMADVIREGIQKAFDTKGWKP"
BASE COUNT         2226 a         2178 c         1741 g         2475 t
ORIGIN      
        1 agaacatcca tgtcgaaaaa gcaccaggag aaacctatga cgtttcaccc ttcgatgtca
       61 tattgatttc ctatggcgtc ggcgatatcg aagcggtgtt aaaaaacacc cttcaacatc
      121 taaaaaacaa cggaaaggta atactcagaa aaccaataac cgaaacaacc gaatacatcg
      181 ataccgtact agaagactat tcgattaaaa aaatcaaatt attgttatcc caagaatcat
      241 atttacttat gaaggcacaa tctgcttcct aagattccct tcgcacctct gattcttttg
      301 ggatctgtga caaatcaatg cgtttcaaat gacaagagat ctttggccgt ggagcaagct
      361 gaatttttgt gatgagttgc ttacgaagag catgggaatc aagttctact gacagaccca
      421 ctcccttcgg tggcgtcaga aactcaaaag agaccgcgtt atgcccaagg agcggcgcct
      481 ggcgtaaggg cacgatttta cattcgccct gtaaaggaac agtcctttct ttatcggtga
      541 aggtgccttc catacgaccc ggaagctcac ttaaaataaa tgccttatag aaccggcctt
      601 tcctatacgg gtcgatatac tcataggtat cggtcgttga ccaaaaccgc agacgtatcg
      661 tcttatcttc taaccgtccc gtaacctcca aatcagaggg ataatacaga tcatactctt
      721 tcacgaacag cactttattg tatttgaacc ggacatcaca gaattcccaa tatcgtttcc
      781 cgtcagcggt caaatataac gctccaatcg aaggaccttc agagacccag aacaatgaat
      841 taccaaaaaa caaactccag gaattgtcaa acacccccca tacccagtca tatccaaaag
      901 gattgttttc tgaagaaaaa ctgattcttt tagggatttt gaatcgatga tgagacaacc
      961 accatcgaat cagatgaccg tacgtcgtaa gggtacgatg cgcccttgag atcgtctgca
     1021 gcgggttctg atacgaccag ttcccatacg catgctccag atatcctttc ccctcaatcg
     1081 aatacgattc ttttcctagt atcatggtcc cgataatatc gcaatgagga aggaaaccgt
     1141 accgatagaa gttcagtcca atcggtagat tcccttgggt cgcctcctga gcgatccaat
     1201 gaggtaacga acgagccgta taatcagcat cgaggataaa accctgcttt ggatcctcga
     1261 catgaagatg gtatttaggg tacaatccct caagatggga ggcctcatag cgaagaccaa
     1321 gtctgttttt cacatgagct aatttcgtaa tctcatcatt gaggtcttta tgtaccaccc
     1381 attttttcga ggtgatatca aagagcacat actggaaaaa acacgaggga gtctcttgtt
     1441 cgtaggcaaa cgaggtcatg aggttccatc gtcggtgatc ctcccgtgtc tgaaagagca
     1501 gttcgctggt ccaccactcc ctcatcaccg ggtcatggtc tccttcttcc tctggaggcc
     1561 agggattctt aggatcatct attgtcatat ttcaaaccta tcattgtttc gacttcggat
     1621 gtacctcaga gctatccatc atggaattat aacctttttt gaaggaaccg gtcttaaaag
     1681 tttaaatgcg gcagttcaat tcctagaaag aacagctgag ttcgtgcaag taacaccaac
     1741 ccaactgcgt tgaccatgct acagaaatta tttgcgcatc tggaaaccat gcgaccgtac
     1801 acgctcctct ggtgtggcct ggtcagtctc cttggtgcta gtctccgata tggggacatc
     1861 ccgccgatac aaatctccct tcttgtcttt ttcatcccga ttctaggatg gattgctggt
     1921 ctgtacctcg cagattacta cgacaaaggg ttagacgcaa tccagaagcc acatcgacca
     1981 atcccctcag gaaggattgt ccctcgcgag gcgttgattg ttggcgcttc ttatgcaata
     2041 ctgggattgg ggcttacctt gttcctcccc ctcatcaaca tcttgttgat tttcattgcc
     2101 ggtgccctgg tcttatgtta tgcgaaatat acaaaagcca aaggactgct tggaaatttc
     2161 aacagaggcg caatgacggt gatcaccttc ctgttcgggg ttttttccat cgatatgaca
     2221 actgcaatcc cgctatctct ctggctactt tcactggtgt ttttcttcca tgacaccaac
     2281 agcaatatca tcggagctat cagagacatc catggggata agactggcgg gtatgcaacc
     2341 accccagtga aatacgggat tcgactcact ctccgcatct ctgtactact cagcatcgta
     2401 tacctcctgc tcactgcagg aatcatatcc ttctacccat tgattttgta tccgacctat
     2461 ctcctgatgt tattcgtcat aggaatcctc attctctgcg tgatgtatat gattttattc
     2521 acatctgtca acaccctaac ccaaaaacaa tcgctgtacg cccatgagtt atttgtcgca
     2581 gaacgcatca tcttttcctc cgcatttatc gtcggaatag catcatccca tatgctatca
     2641 tttaccttat gtatcgtctc tctagtcacg accctgcttt cgcaacatct cctcagagaa
     2701 cggtacgagt taacgtaggt actatgaatt gcttagtaac cggagcctct ggcttcatag
     2761 gatttgcact ggtcaaacga cttgtacagg acggtcatca tgtacgagcg attctccata
     2821 aaacctcccc accgcatcgt gagacacagg tcgattatat catcgcagat ctcactgatc
     2881 ctgtttcgtt aacaccctta gtccatgacg ttgacatcgt cttccattgt gctgcacatg
     2941 tgaaggattt cggatcgaaa aaagaaatca tggacgtaaa tctaaaggga acacaacatc
     3001 tggtcaaagc ctgtggaaac acgatccgtc agttcatttt tctcagccat cttcacggca
     3061 catcaccaac atacattgga ccgtacagct cctcaaaagg actcgcagaa caatatcttt
     3121 tacaacaaca tcagaacaac cagttccctg tggtgatcat cagaccaggg agtgtgtttg
     3181 gacctggtgc gaccacatgg tgccttcgtc cacttcaagc aatccagcag gatcgcatcg
     3241 ccctgattga ccatggcact ggcatttttc ttcataccta catagagaac cttctcgatg
     3301 gtcttctgtc cattcccacc acatcacacc tggagggaga aatcatcgaa atcacagatg
     3361 gagataacac gaccacctgg aaaacctacc tcaacgacct tgctgctctc gctggaaaac
     3421 aaccaatcac gcggaacatt tcaaaaacca ctgcctcgtt gatcagtcat tctatgatga
     3481 tccgatacta tctcttccat aaaaccccac tgctcactcc aaccgcggta caccttttca
     3541 ctaatcatcg tacggtttca attgaaaaag cacacaaact cctcgggtat tcaccaatgg
     3601 tagactaccc tgaaggaatg aaacgcatag cccaatggtt acaaaaggaa cagtatatct
     3661 gagaaaagtg ctctgctttc gaaacctatc agaagttaaa atctcttttg taataccgtc
     3721 ggaacgtata attcgtcccc gctggcagga ccgcaggtag attccgtacg atcgcgaatg
     3781 tccgaagagc acgcataatg acattaggca tgctatagta tttttttgcc actttccggg
     3841 cgccttcgaa gagttctttt tctgtcatat gtttcggttt gaataccaca tcgacttgat
     3901 tgtaccgctt ccaatcttta ctaatgatcc gtccttctcg ctccagccga tcgtacaacg
     3961 gtgtcccagg aaacggtgtc agaatattga tttcaacagc atccaactcc cactcattca
     4021 tcttctcaaa cgtcaaatca aagatttcag gggtgtcctg gtcgaaacca aagatgatgc
     4081 ctccttgaac agccatccca tgtttatgta atcgtttaat ggttttcttg aaatcttcaa
     4141 ccttattgac tgttttttta atcccgttta acgcttcctg gctgacggat tcaaatccga
     4201 cgaaccattc gacacaccca gatttttttg ccagatccag gaattcatca tcgattttcc
     4261 caagcacatt ggaattgccg tttgataccc atcctttgtg cactttttgt ctgataagct
     4321 ccttaaaaag ctcgcgtgaa tatgatggat tcaccgtcag actcgggtca tggatgacaa
     4381 agatccgatt cggcatctgt gtgatttcat caacaacgtt tttcaccgga cgatgtttca
     4441 ccgcatggtt acagaaactt gcgatagcgc aaaactcgca ttgattcggg catcctctgg
     4501 ttgactgcac cgcaccaatc atcgggttct gacggataag atcacgtctg attgggggaa
     4561 tctcagccat atcaaaatca gggttcggcc catagaattg tttcagcact cctttctcca
     4621 catcatgaat cagctcaggc caagtcagtt cagcttcccc aagaaccacg ctatccgcat
     4681 gttgtatggc ttcctgtggc atcgcagtag gatgatatcc acccagcacc actttttttc
     4741 cacgtctgcg aaactcatcg gcaatctcat agacccgtgg tgcggtcatc gtataactcg
     4801 tgatacctac aaggtctgtt ggttcatcaa aatcaatatc ttcatagttc tcattgacaa
     4861 ttttcacggt atgctgtcgt ggggtgatcc ctgctagatg cggtaaggtg atgataggat
     4921 acccaaatag aatcgagctc cacgatcctt tatcagaata tgtcgttgct ccatgttcaa
     4981 ttcgtgggaa aaccagtagg atgttcataa gtcaccatgg tatggtatct gtggatattt
     5041 aacatttttg acatgatacg atgacaagaa accatctctt tctacgaacg atcatgggat
     5101 cgttcttggt tcttttccta tgtacaccgc ttcagtgaag tttatctgac gcatgcgagg
     5161 ttgtgaagcg acttcttcga agtaatgagc agaaagacca gctattcgcc cgtagataaa
     5221 gaacgctttc gccatcgcgg gatctagttt catatctgct acgatcgccc caattacacc
     5281 atcgacattg atagggagac tcattcctcg aacctgttca aatatcgtac tgactttctt
     5341 ggatagttgt acatgttcgc cagcgatacc tgtttgagac gataaattcc aaagaacatc
     5401 acgtcgtgga tccttcgtat gaagtcgatg ccctaaacct tcgatacgtt ttccttcttc
     5461 gatgtatttt ctcattatct ctcgggtcgc ttgggcgaca tcgatgtttt ccttttttga
     5521 tttcatgaaa cactcgatga agagctgcgc ggcctttgca ccagcgccgc cgtgcacatc
     5581 ggtaatcgca cctacaccct gagcaaccgc cacttcgtag ggtgctcttg tggatgccgc
     5641 gataagagtc gcttgggcag atggtggtgt gaccccatga tctacacagg ctactatcat
     5701 cgcctcgatc atgcgagaat gctgttcatt caccgccgta ttgcctgtca ccgcacagta
     5761 aaccacatgg gagaatggtt catttccaga aagcttttct aaagcttctt ccgtacctaa
     5821 tatcccgctt aggtatcgag cagttctgcc gataccgaag atagtttttt tcactgcccc
     5881 attcgttcct tcctcaggat actgtgcgag ctcctcgtca agaacaaaat actttaccaa
     5941 tgtttttgag atatcttcct tttttgatcg gctcaccttt ggaacaggtt tttttgctgc
     6001 ttctactgca atcttccgca tctcttcata tgttttttta tcaggaaact ctccttttac
     6061 aaggaggtat gccgtttcaa ggaatccctt ctgttcgacg atgtcctgta atggataacc
     6121 agaaatcaac agattgttcg gcatcacttt cgtaatgagt gttccccaac cacgtttctt
     6181 ggaaggggca tcccagatcg ttttcatttt cttataataa ttctcaacat caaaatagat
     6241 tttcttctgc aacctttttt gaacttcagt tatgagatcg tactgactgt tcacgacagg
     6301 aatcccaacg ctttcaagtg cttgtttttt tgattggaat gttccataga cttgcccggg
     6361 ggagacgatt gcccccgcat ggcccatggt ttttccttca ggagcatgtg cccccgagat
     6421 taaagcaaca agaggttttg gatatttttt tgggttcttc tttatgtcgt cagcgaggag
     6481 ttcttcttgg catccaccga tttcaccggc gacaacgacg aggtctgtgt ttttatatgc
     6541 catcgcaaga ggaacgaggt cgcggaatgt cgagcctatc gcaccgtctc cgccgacacc
     6601 aagaaccgcg ttttgtgcga taccaacgct tgctaatgca tcagagagat gatacaagat
     6661 ggctccactc cgggaaatga tggtgacgcc tttcggacca aattcttctg gagaaacctc
     6721 ctcagggtag aacacatcag ggagcatccc agctttgata ccttctggag gaaaaatgac
     6781 accaggggtg ttcccaccga tgagtactgt tttgttttta cgtgcagcga caaggatatc
     6841 ccggacatct ttcaaaggga tcccttcact gataagaatg accagtggga tgcctgcttc
     6901 gattgcttct atcgtcgcat cctttgctgt cgagtaatgt cgccagacgc ttgcgaccgc
     6961 gatgttcgga tgttctttga ggcattcgga aatcgtgtcg tacaccggta tcgtctccaa
     7021 gagtcgtgtt cctccgcgtc ctggtgcgat acctgcggta atcgtggtcc cgtactgctg
     7081 catgagctct gcatgtcgtg tcccttgccc tccaagaccg accactatag tctctattac
     7141 cccggattgt ttgatttttt ttgcaagatc aggtcgtttg ttttcgatga tgtagtacag
     7201 catcccctga tctgggggga ccagcttcat ggtttccacc ccttagtatc aaacgctttt
     7261 tgaatcccct ctcggatcac atcagccatg atccgctggt tccctacgta gattgtgacg
     7321 ttttgtaacg cctcagagct ctctgctttt gctttttcta agaaagcccg tgcactagga
     7381 agatcggttc caaccatcct accaaaaacc ggtaccattc gcttcccgct ttcataacgt
     7441 tctttcactg cttgtaccat accgcgtata aaaatatcac aacttgagat accaccgaac
     7501 cgtgaggtaa caatcagatc gaccactgga taatccatga ggagatgaaa catctcagca
     7561 acccggtttt gcgaaggacc accaccgcta tccatgaaat tcacaggaac tactcgatca
     7621 tggaaatagc gattcccgat tgtcgacacc tcatcaatgg aaaaaatccc atatcctgcg
     7681 ccgccaggga taaggccgac gtataatttt ttctgatctt tcgtaaaggg ctgaggtaac
     7741 agatcgatgt atggaaaccc agcggactga gcccgaagtt cattcttcgt ccgttccgcc
     7801 acatcatgtc gtttttctgt gatgccaagc atttcgagca gttcgacttg tctgaagagc
     7861 gcattgtcat caagcacaag ctttgcatcc gctgcaatag caccattgga ggtgataatc
     7921 aaagggttga tctcacagag tcgtgcatcg aatcgttgat aagtcgcata gactttcgcg
     7981 atgatttctg caaggatttt tgtactctca ttttttcctt ttaaaaattg gttcagagac
     8041 gatgcaagat cctgtgctat tgtcttcgaa atttctttat tattatcggg gatctctttt
     8101 ctcacaatag cctgtgggtt tttcattgcg acctgttcga tgtcgactcc accggaggca
     8161 ctaacgatga cgacgttgtt aaatgtcgct ggatccatgg tgatcccaac ataacaagcc
     8221 ccgatttctt ccacagcctc ctcaagaaga atgcagtcca ctggaaatcc ttttatttta
     8281 agtgtaaata aaacagcagc aatttttcgt gcttcttcta cattatttgc ttttttgata
     8341 ccaccagctt ttcctctacc accgactagg accattgatt tcaacatgat ggggaattcc
     8401 agttttagcg tatcgagttc ttttagagaa cgaataacac catattgttt tgggatagga
     8461 atacccatct tctcgaaaac tttctttgct tcgaattcgt acagtttcat ttgactagtt
     8521 ctcctttcct ttgcaatgtt ttctttattt caaaatcgat aaaatcgcaa tgatgaatga
     8581 taatcgcctc cggtgtccta ttcatagtat cgccttcatg
//
LOCUS       DATMXJ010000184         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_8673, whole genome shotgun sequence.
ACCESSION   DATMXJ010000184 DATMXJ010000000
VERSION     DATMXJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8673"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VN377_04845"
     CDS             <1..366
                     /locus_tag="VN377_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27745.1"
                     /translation="INQSATRDFYFPEINGSIPQINFTVICKHKLISPVIIPRFTQVY
                     IAISYNGTYILLSQSNNHRCQSLEWEYINLTVDSSNGFIPLVTNGENITLNVEVGAYF
                     FVFGAVEQLAPITIHPISQ"
     gene            complement(429..2393)
                     /locus_tag="VN377_04850"
     CDS             complement(429..2393)
                     /locus_tag="VN377_04850"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27746.1"
                     /translation="MCFHSSFQAKETNVIWSCTIHVDESGGKLDDIVFGEAPDANDGS
                     PADSYDVVKPPTPMIPYLKTTLTDNLPAPYDSLWKDYRQYPDTSKIWNVTVQWMPSSG
                     SSPTTITMSWITFELDESEYTSITLCTDTGTVLKNMLIDNSYTFSCPAYVPQYFKIRC
                     ERTNNPPETPSTPVGETTGYHGISNAYVTSCIDPNNDNMYYQFDWGDGIMSSWLGPYS
                     SGQTIQASYIWHTPGTYQVKAHTKDIYGQQSTWSSALTIEMLNRAPAPSTDPSPQNGV
                     PNVQRSPNLTWTGTDPDRDLITYNVYFSTTNPPAKLVDNQSDSSFCPGILLNQTTYYW
                     KIISWDSFGGNTSGPLWSFTTRKDNGSSEPPDSDANQTNHPPVADASLSEQTGFVGSF
                     LIFNGSRSYDTDGYLTKWSWDFGDGTNGTGERTIHTYQALGIYTVILTVTDDMGATGT
                     DTISVEIGSANWPPTKPMINGTNVGTKNQTYSYTISATDADNDFLQYIVNWGDGAQNT
                     SVFLPNGTIWSLSHSWNASGKYQVTVKATDNNTFSEPAIMEVFIDVSFVSTLGFLFDA
                     DNNGQVDSFYINGTGVITSVQRTDDGSYYLDTDNDGKWNYLYTPNNGSLTPLNPSVTT
                     IENPWIFIGIIAIAIIIIGCIVYLYKKNYF"
     gene            complement(2456..>2764)
                     /locus_tag="VN377_04855"
     CDS             complement(2456..>2764)
                     /locus_tag="VN377_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27747.1"
                     /translation="IPMLRVVIFILILFMVIIVLLLLYRKRKKSRSSENKISQTLDEQ
                     KSEALQEKRLQKLEENKSQALEKKKSQISTKKKSPALKKNKSQVSEKKQKKSQKKRKR
                     "
BASE COUNT          721 a          546 c          595 g          902 t
ORIGIN      
        1 atcaatcaga gcgctaccag agacttctat ttccctgaga taaacggttc gatcccacaa
       61 ataaatttca cggtcatctg caaacataag ctgatcagcc ccgtgatcat tcctcgattc
      121 acacaagtgt atattgcgat atcttacaat ggtacgtata tcctgctgag tcaatcaaat
      181 aatcatcggt gtcaaagcct agagtgggaa tacattaatc tcacggttga ttccagcaat
      241 ggatttatcc ctcttgtcac gaatggtgaa aacataacac tgaatgtcga agtcggagcg
      301 tatttctttg tctttggggc tgttgaacaa ttggctccta tcacgattca ccccatatca
      361 cagtaacaga gatatcctct gagaaaagag agacatacga gatttctatc caccgatacg
      421 tcctttattc agaaataatt ttttttgtac agatagacga tacagccgat gatgataata
      481 gcgatagcga taatacctat gaagatccat gggttttcaa tcgtcgtaac actcggattt
      541 aatggagtaa gcgaaccatt atttggagtg tagagatagt tccatttccc atcattatct
      601 gtatcaagat aatagcttcc atcgtctgtc ctttggacac tggtgatgac cccggtaccg
      661 ttgatataaa acgagtcgac ctgtccattg ttatctgcat caaaaaggaa tccaagtgtt
      721 gagacgaagg atacgtcgat gaagacttcc atgatagctg gttctgagaa cgtgttgttg
      781 tctgttgctt tgacagtcac ttgatatttt cccgaagcgt tccatgaatg tgacagggac
      841 cagatcgtcc cattcggaag gaacacgctt gtgttttgtg ccccatcacc ccagtttacg
      901 atgtactgaa ggaaatcgtt atccgcatca gttgcggata tcgtgtatga ataggtctgg
      961 ttttttgtcc ctacgttggt tccgttgatc attggttttg tgggaggcca gttcgcagaa
     1021 ccaatctcaa ctgaaatcgt atctgttcct gttgctccca tatcatcggt tacggtaagg
     1081 atcacagtat agattcctaa agcttgatag gtatggatgg ttcgttcacc agttccattt
     1141 gtgccatcac cgaaatccca tgaccatttt gtcaaatacc catcagtgtc ataactccgt
     1201 gatccgttaa aaatcaggaa actaccaaca aatcctgtct gttcactcaa ggaggcatct
     1261 gcaacaggcg gatgattggt ttgattcgcg tcactgtccg gtggttctga ggagccattg
     1321 tcttttcgtg tggtaaaaga ccaaagagga ccactggtgt tacctccaaa gctatcccac
     1381 gagatgattt tccaataata ggttgtctgg ttgagtaaaa tccctgggca gaaggaggaa
     1441 tcggattggt tatccacgag tttcgcagga gggtttgtgg tgctgaaata cacgttatag
     1501 gtgataagat ctctatcagg gtctgtccca gtccaggtga ggtttgggct tctttgaaca
     1561 ttgggcaccc cgttttgtgg tgaggggtct gtcgatggag ctggtgctct gtttagcatt
     1621 tcaatggtga gtgccgacga ccaggtactt tgttggccgt agatatcttt agtatgtgct
     1681 ttcacctgat aggttcctgg agtatgccag atgtacgatg cttgaatggt ctgtccacta
     1741 gagtaggggc caagccagct gctcatgatg ccatcgcccc aatcaaactg gtaatacata
     1801 ttgtcgttat taggatcaat acacgacgtc acataagcat tggaaattcc atgataacct
     1861 gtagtttcac caactggagt acttggtgtt tctggcgggt tgttggtgcg ttcacatctt
     1921 attttgaaat attggggaac atatgcggga caactgaatg tatatgagtt atctatcaac
     1981 atatttttta aaacggtccc tgtgtctgta cagagggtaa tcgatgtata ttcactttca
     2041 tcaagttcaa aggtaatcca tgacatggta attgtggtag gggatgatcc gctacttggc
     2101 atccattgta ccgttacatt ccaaatttta gaggtatcgg ggtactgtcg gtaatccttc
     2161 cataaactat cgtatggtgc ggggaggtta tcagttaagg ttgttttcaa atatggaatc
     2221 attggtgtag gaggttttac gacatcgtaa ctatctgctg gtgatccgtc atttgcatct
     2281 ggtgcttcac caaacacgat atcatccagt tttccccctg attcgtctac atgtatagtg
     2341 caactccata taacattggt ttctttcgct tgaaatgaac tatggaagca catacaaata
     2401 ttagttagaa aaaagagact tactacgaaa acatctattc tgtttttcat tggtattacc
     2461 ttttacgctt tttttgagat tttttttgct ttttctcaga tacctgtgat ttatttttct
     2521 ttaatgcagg tgatttcttt tttgttgata tctgtgattt ctttttttct aatgcctgtg
     2581 atttattttc ttctaatttc tgtaatctct tttcttgtaa tgcctctgat ttctgttcat
     2641 ctaatgtctg tgatatctta ttctctgatg accgtgattt ttttctcttt ctatagagga
     2701 gtaataaaac tataattacc atgaataata taaggataaa aataacgaca cggagcattg
     2761 gtat
//
LOCUS       DATMXJ010000185         6738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50648, whole genome shotgun sequence.
ACCESSION   DATMXJ010000185 DATMXJ010000000
VERSION     DATMXJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50648"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VN377_04860"
     CDS             <1..783
                     /locus_tag="VN377_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27748.1"
                     /translation="CMAIFAPFIAPQDPTPSPPKLENSLKPGFWSNESIPGYPLGTDM
                     MGRDILSRIIYGSRISLTVGFIAVGIATALGILIGVAAGYYGGWIDNILMRITDILFA
                     FPSLLLAIVIVAVIGQGLDKAMIAIGIEYSPQMARVMRGSVLYIKEMEYIEAQKAIGS
                     SNTRIIFRHVLPNSLSPVIVYGTLMMAGAILDAAALGFLGLGAQPPSPEWGAMLSESR
                     FYIVSGSWWAATFPGIAIIIAVLGLNLLGDGLRDILDPRLKE"
     gene            795..1766
                     /locus_tag="VN377_04865"
     CDS             795..1766
                     /locus_tag="VN377_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27749.1"
                     /translation="MEELLRVEDLKTYFFTHEGTVKAVDGVSFTINKGETLGLVGESG
                     CGKSVTALSIMRLIQTPPGKIVGGKIFLEGTDLLKLSEKEMRNIRGCKISMIFQEPMT
                     SLDPMFTIGSEIMEVLKLHHKLKKDEARKKAIESLYTVRFPDPEKRIDDYPHELSGGM
                     RQRVMIAMALSCNPALLIADEPTTALDVTIQAQILKLIAELRKEFDTSVLLITHDLGV
                     IAKTCDNVALMYAGYMVEYTDVHTFFKQPLHPYAQALLKSIPRLDIDTERLKIIKGLV
                     PNLLDLPTGCPFHPRCDYCFEPCTKTLPPLIKAGERHWVRCYLIGGL"
     gene            1770..2732
                     /locus_tag="VN377_04870"
     CDS             1770..2732
                     /locus_tag="VN377_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27750.1"
                     /translation="MDHLIEVKNLKKYFYQGGGPFKKKNAIKALDDVSFYIKRNEILG
                     LVGESGCGKTTCGKLILRLINPTAGTISFDGHDITHLKQKQLTEYRRKMMIIYQDPYG
                     SLDPRMTIGAAIAEPMEVHKLTKTKEEKENKIVELMEKVGLTPDQINRYPHEFSGGQR
                     QRIGIARALATNPTFIVADEPVSALDVSIQAQIINLLKDLQKDFGLTLLFITHDLSVI
                     KHTSDRIAVMYAGKLVELASKQDLFTDPLHPYTRALLSAIPVPDPERQTQYIPLTGEV
                     PSLIHPPPGCRFHPRCTEARPECSAREPELREVKKDHFVSCFPS"
     gene            2865..4547
                     /locus_tag="VN377_04875"
     CDS             2865..4547
                     /locus_tag="VN377_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWR27751.1"
                     /translation="MGSFKKKDMKAGMFAIIICAMFLTIAFSGCTQQQQNGGKTEIKN
                     PTTIVRYDIGDAKTLDPADCYDTASSEPIFSIYDTLVTYKGNNTKTFYPSLATDWTVS
                     NDSLTWTFNLRHNVKFSNGNDFTAEDVKYSFDRVLTMNAPESGVDWILGQCMDTNSTT
                     IIDDYTIQIHLTHPYGGFLALMPYTVAAIVDKDYVEANGGVVANQENAWMKEHPMGTG
                     PYMLNHWTRATEIVLDKNPTYWGGWEGKHVDKAVIKYADEAATRILALKNGDADFAYV
                     PYANLKDVIGEKGIVVHPFDSYDVALLAINTKANNSQYLTDGRVRKALSYAFDYDTAI
                     KSAWSGYAARLAGAIPKGMPYYETQNNGQPYYNYNLTMAEQILDDAGYLKDYDLSGTM
                     YRFNGTTIRLFYNAGNSEREMMAVMFRAALDQIGILSSIIAEGWPQLLHRMYTTDDWD
                     TMFIGWGPDYNDPDDYIAPFMGSADIGQDTYNTGWKNATVDEKILEGKYSADSAVRAA
                     AYKAAFDIYIQEPSFIFLVQRAYIRPWRDWVQNYSYNPVREWYFYDYYKAYP"
     gene            4579..5601
                     /locus_tag="VN377_04880"
     CDS             4579..5601
                     /locus_tag="VN377_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27752.1"
                     /translation="MKTWEFIVRRLLLLIPVIIGVTIITFTISHVIPADPARAFAGGP
                     KAQPDVIERITKELHLDQPLWIQYFYYLNDIIHFNLGKSYLESRPVVESLAQYFPATI
                     ELTIFAMLIAIPLGIVGGIISAIRRNKLSDHLTRIIAIVGYSLPIFWLALMLQYIFAY
                     QFPILPLEGRLAIGMNAPTSITGLYVLDSILTGNLATLGSALYHLIMPAFTLGFATLA
                     LILRMTRSSMLEVMGMDYIRTGRAKGLSERTIIYKHALRNALIPTVTVTGLAFGGLLG
                     GAVLTETIFNWPGMGRFAANAVLGFDFNAVMGFTILVAIIYVIANLIVDVLYAVIDPR
                     VRLGGG"
     gene            5675..6547
                     /locus_tag="VN377_04885"
     CDS             5675..6547
                     /locus_tag="VN377_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWR27753.1"
                     /translation="MAHLFRKSLLSMIGLILILILVIVALFAPFIASQHPSYVRVSTE
                     QGTTVIEERWDINFSQTLLPPSAEHFFGTDDYGRDIFSMVIYGSQTSFRICIIVVAIS
                     TLVGIILGGIAGYFGGIIDEVLMRITDVFLAIPYLILAMAVAAALGRSIDHIMEALII
                     VWWPTYARLMRGQVIAIREQQFVEAARSVGASNSRILFRHIFPNSFAPLLVEITLDLG
                     AVLLVAAGLSFIGLGASPGTAEWGLMISSGRIHMFHAWWYVTFPGLAILLVVLGFNLL
                     GDGLRDVTDPKLRR"
     gene            6627..>6738
                     /locus_tag="VN377_04890"
     CDS             6627..>6738
                     /locus_tag="VN377_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWR27754.1"
                     /translation="MEETSFQIWKGETFGLIGETGCGKSVTALSVMGLIPQ"
BASE COUNT         1977 a         1432 c         1561 g         1768 t
ORIGIN      
        1 tgtatggcaa tcttcgcccc cttcatagcc ccccaggacc caacaccctc acccccaaag
       61 cttgaaaata gtctaaaacc aggattttgg agtaatgaaa gcataccggg gtaccctctt
      121 ggaacagaca tgatgggcag agacatccta agcagaatca tttatggctc cagaatcagc
      181 ttaacagtag gtttcatcgc ggttggtatc gcgacagcac tcggtatttt aattggggtt
      241 gctgcggggt actatggcgg atggattgat aatattctta tgcgaatcac tgacatcctg
      301 tttgccttcc cgtcattatt actcgccatc gtcattgtcg cagtaattgg acagggatta
      361 gacaaagcta tgatcgccat tggtattgaa tactcacctc agatggcacg agtcatgcga
      421 ggctcagtac tatacattaa agaaatggaa tatatcgaag cccagaaagc gattggctca
      481 agcaatacaa gaattatttt tcgacatgtg ctgccgaata gcctttcacc ggttattgtc
      541 tatggaacat tgatgatggc aggtgcaatt cttgatgcag cagcacttgg tttcttagga
      601 ttaggcgcac aaccaccaag cccagaatgg ggtgcgatgc tctccgaaag tcgcttctac
      661 attgtaagcg gctcatggtg ggcagcaacc ttcccaggaa ttgctattat cattgcagta
      721 ttaggactaa atctgttagg agatggttta cgagatattt tagatccaag actaaaagaa
      781 taggatgaaa aaaaatggaa gagctattga gagttgaaga tctcaaaaca tactttttca
      841 cccatgaagg aactgtcaaa gcagtcgatg gggtgagttt caccatcaac aaaggggaaa
      901 ccctcggcct tgttggagag tcagggtgcg ggaaaagtgt taccgcgcta tccatcatgc
      961 gattgataca aaccccccca ggaaaaatcg tgggcggaaa aatattcctc gaagggacag
     1021 atcttttaaa gttaagtgaa aaagaaatgc gaaacatccg tggatgcaag atatcgatga
     1081 ttttccagga accaatgaca tcccttgatc cgatgtttac cattggttct gagatcatgg
     1141 aagtgttaaa actgcatcac aagttaaaaa aggacgaggc acgaaaaaag gcgatcgaat
     1201 cactgtacac cgtcaggttc ccagacccag aaaaacggat agacgattac ccccatgagc
     1261 tgtccggggg gatgcggcag cgggtgatga tcgcgatggc gttatcctgc aaccctgcgc
     1321 tcctgatcgc tgatgagccg accactgccc tggatgtgac gattcaggct cagatcttaa
     1381 aacttatcgc tgaacttcga aaggaattcg acacctcggt gttactgatc acacacgatc
     1441 taggagtaat agcaaaaacc tgtgataacg tcgctttgat gtacgcagga tacatggtcg
     1501 aatacaccga tgttcacacg tttttcaaac aaccgctgca cccttatgca caagcgctac
     1561 tgaaatcgat cccacggctt gatatcgaca cagagcgttt gaaaatcatc aaggggctcg
     1621 ttcccaacct tcttgaccta cccaccggtt gtccgttcca tccaagatgc gactattgtt
     1681 ttgagccatg cacaaagacc ttaccaccac tgataaaagc aggagaacgt cattgggtgc
     1741 gatgctactt aatcggaggt ctctagtaca tggatcattt aatcgaagta aagaacctga
     1801 agaaatattt ttatcagggt ggtggaccgt tcaaaaagaa aaatgcgatt aaagcacttg
     1861 atgatgtgag tttttatatc aaaagaaatg aaattcttgg tcttgtggga gaatcaggat
     1921 gtggaaaaac cacctgtgga aagctcatcc tccgattaat aaatccgaca gcaggtacga
     1981 tctcctttga tggacacgat atcactcatt taaaacaaaa acagttgacg gagtatcgga
     2041 ggaaaatgat gatcatctac caggacccct atgggtctct tgacccaagg atgacgattg
     2101 gggcagcgat cgcagaaccc atggaagtcc ataaattgac gaaaacaaaa gaggaaaaag
     2161 aaaacaaaat cgttgaactg atggaaaaag tgggattgac ccccgatcaa ataaaccgat
     2221 accctcatga gttcagcggt ggacaacgac agaggatcgg tatcgcacgg gcgttagcga
     2281 ccaatccgac ttttattgtc gcggatgaac cggtatccgc ccttgatgtt tccatccaag
     2341 cgcaaatcat taatctgttg aaggatttac aaaaagattt tgggttgact ctcctcttta
     2401 tcacgcatga tctcagcgtc attaaacaca cgagcgatcg tatagcagtg atgtatgcgg
     2461 ggaaattggt ggaactagca tcaaagcagg atttatttac tgatccatta catccgtaca
     2521 cacgagcgtt attgtctgcg attccagtcc ccgacccaga gcgtcaaaca caatatatcc
     2581 ctttgaccgg tgaggttcct agtctcatcc atccgccacc tggctgtcga tttcacccac
     2641 ggtgtaccga ggcaagacca gaatgttcag caagggaacc agaactccga gaagtgaaaa
     2701 aagatcattt tgtttcttgt tttccgtcct aaagtcagtc gaacgaggca tttatcgtat
     2761 cttccaaagt actatttaga ataaaatgaa aaagtaatta aataatatgc gtgatacgaa
     2821 cataggtctt ctctaagaga agcgggaaaa gaggtcagaa aatcatggga agttttaaaa
     2881 agaaagatat gaaggctggt atgtttgcga tcattatctg tgcgatgttt cttaccatcg
     2941 cgtttagtgg atgtacacaa cagcagcaga atggcggaaa aactgaaata aaaaatccaa
     3001 caacgattgt gaggtatgac atcggtgatg caaagacgct tgacccagca gattgttatg
     3061 atacagcgtc ctcagagcca atcttttcta tctatgacac gctcgttacc tacaagggaa
     3121 acaacacgaa aacgttctat ccaagtcttg cgacagactg gaccgtatca aatgactcat
     3181 taacatggac atttaattta cgtcacaatg tgaaattttc caacggaaat gattttaccg
     3241 cagaagatgt gaaatactct ttcgatcgag tgttgacgat gaacgcaccg gaatctggtg
     3301 tggactggat tctcgggcaa tgtatggata cgaacagcac caccattatt gatgactata
     3361 ccattcaaat acaccttaca catccctatg gtggattttt ggctctgatg ccatatacgg
     3421 tagccgcgat tgtcgataaa gactacgttg aggcaaacgg tggtgttgtc gcaaatcaag
     3481 aaaacgcatg gatgaaagaa catccgatgg gcaccggtcc atatatgctt aaccactgga
     3541 cgcgggcgac ggaaattgtc ttagacaaaa acccgacgta ctggggtggt tgggaaggaa
     3601 aacatgtcga taaagccgtg ataaaatatg ctgatgaagc ggcaacacga attttagcgc
     3661 tgaaaaatgg ggatgctgat tttgcctatg tcccctacgc aaacctcaag gacgtcatcg
     3721 gtgagaaagg gattgttgtt catcccttcg acagctatga tgttgcactt ctcgcgataa
     3781 acacaaaagc gaataatagt cagtatttaa cagatggtcg agtacgaaaa gcattaagtt
     3841 atgcgttcga ctatgataca gcgatcaaat ctgcctggag tggatatgca gccagactcg
     3901 ctggcgcaat accaaaggga atgccgtatt acgaaacaca aaacaatggc caaccatatt
     3961 ataattataa cctgacaatg gcagaacaaa tacttgatga tgctggatat ctgaaagatt
     4021 atgatctaag tggaacgatg tatcggttca acgggacaac cattcgactg ttctataacg
     4081 caggcaattc agaacgggaa atgatggcgg tcatgttccg agcagctctc gatcaaatcg
     4141 gtattctatc atcgattatt gcggaaggat ggccacagct tctgcatcgg atgtatacga
     4201 cagatgactg ggatacgatg tttatcggat ggggaccaga ttataatgat cctgatgact
     4261 atatcgcacc cttcatgggc tcggctgata ttggacaaga tacctataac acaggatgga
     4321 agaatgcaac agttgacgag aaaatattag aaggaaaata ctcagcggat tctgcagtgc
     4381 gagcagctgc ttataaagca gcattcgaca tctacattca agaaccatcc ttcattttcc
     4441 tggtacagag agcatacatt cgaccatgga gagattgggt acaaaactat tcgtacaacc
     4501 cagtgcgtga atggtatttc tacgattact acaaagcgta cccataaaac catctctttc
     4561 tttctttttt tttaagttat gaagacatgg gaattcatag tccgaagact cctgctcttg
     4621 ataccggtca tcattggagt cactatcatt acgtttacca tttcccatgt tatcccagca
     4681 gacccagcac gagcctttgc aggaggacca aaagcacaac ctgatgtcat cgaacgaatt
     4741 acaaaagaac ttcatcttga tcagccactc tggattcaat atttttatta tttaaatgat
     4801 atcattcatt tcaacctagg aaaatcatat ctggaaagtc gaccagtggt cgagagcctt
     4861 gctcaatatt tcccagcgac gatagaactg acaatttttg caatgttaat cgcaattcct
     4921 ctgggtatcg tgggaggaat tatttcagcg atacgacgaa ataaattatc agatcattta
     4981 acacgaatca tcgcgatagt tggatattcg ttgccgattt tttggttggc tctcatgctg
     5041 caatatattt ttgcgtatca atttccgatt ttgccgttgg agggacggtt ggcaatcggg
     5101 atgaacgcgc ccacctcaat taccggttta tatgtgcttg atagtatttt aacggggaat
     5161 ctcgcaacgt tagggtcagc tttatatcat ctcatcatgc cagcgttcac gttaggattt
     5221 gctacccttg cactgattct tcgtatgaca cggtccagta tgttggaagt catggggatg
     5281 gattatattc gcacggggag agcaaaagga ttatcggagc gaacgattat ctataaacac
     5341 gcattgagaa acgctttgat accaacagtt acggtcaccg gcctggcatt tggtgggctg
     5401 cttggtggtg ctgtactaac agaaacgatt tttaattggc ctgggatggg acggtttgct
     5461 gcaaacgcag ttttgggttt tgatttcaac gcagtgatgg gatttacgat acttgtcgca
     5521 attatatatg tgatagcgaa tctcatcgtt gatgtcctgt atgcggtgat tgatccacga
     5581 gttagactcg gaggtggata agatacgacc gcatgttaaa gtcatggatg atttcctaaa
     5641 aaaacatgaa tcaagaatta aagatctgag attcatggct catctcttcc gaaaaagtct
     5701 gttatcgatg ataggattga tactgatact tatcttggtt attgtcgcat tatttgcacc
     5761 attcatagca agccaacacc cgtcttatgt aagagtgagt actgaacagg gcaccaccgt
     5821 catcgaagaa cgatgggaca ttaattttag tcaaacatta ctgcccccta gcgcagaaca
     5881 tttctttggg actgatgact atggacgcga catctttagt atggttatct acgggtctca
     5941 aacatcgttc cgtatttgta ttatcgttgt tgctatatca acgttagtcg ggattatcct
     6001 tggggggatt gctgggtatt ttggtggaat catcgatgag gtattgatga gaatcaccga
     6061 tgtcttcctc gcaataccgt accttatttt ggcaatggct gtcgcggcgg cattggggcg
     6121 aagcatcgac catatcatgg aagcgttgat tattgtctgg tggcctacgt atgctcgttt
     6181 aatgcgaggt caagtaatcg caatccgtga gcaacaattt gtcgaagcag cacgaagcgt
     6241 tggtgcgagt aactcacgta tcctcttccg tcatatcttt ccaaattcct tcgcaccatt
     6301 actggtggaa atcacattgg atcttggagc cgtgcttctg gtagctgcgg gactcagttt
     6361 catcggattg ggagcatccc cgggaaccgc ggaatggggg ctgatgatat cctctggtcg
     6421 aatccatatg ttccatgcct ggtggtacgt caccttccca ggcctcgcaa tactgcttgt
     6481 cgtcctcgga ttcaacctcc ttggtgatgg cctgagagat gtgaccgatc ctaaattacg
     6541 aaggtgattt catagaaaac gaaccattgc tcaagataaa agacctgaaa acctacttct
     6601 acacctacga aggagtcgtc aaagcattgg aggaaacaag cttccaaatc tggaagggcg
     6661 agacgttcgg gttgattggg gaaacgggat gtgggaaaag cgtcaccgca ctctccgtga
     6721 tgggtctgat ccctcaac
//
LOCUS       DATMXJ010000186         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_77082, whole genome shotgun sequence.
ACCESSION   DATMXJ010000186 DATMXJ010000000
VERSION     DATMXJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77082"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VN377_04895"
     CDS             <1..838
                     /locus_tag="VN377_04895"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27755.1"
                     /translation="SSDLSNWQMGRSHAHSKDQMAPMIDWDGGTWREIVQCCLLFGDP
                     AQQLRTPHPSEAPSPPSKPVGEDLGIWNVEYCYTSQMGSDPENDQIFCLFDWGDGSNT
                     GWLGPFNGGETVTACHIWTALGTYEVKVKARDVWGAGSTWSESLVVTITDNTPPDAPE
                     VTGPSEGKPGNPYLFNMITSDPQDQNIYYFVDWGDNTSTGWLGPYLSGTEIHQTHSWA
                     EKGTYTVKVKAKDIMDSESEWGTVSIVMPTEYTFSLLGFIQHLLGMFPNLFPVLRHLA
                     GY"
     gene            complement(1087..1692)
                     /locus_tag="VN377_04900"
     CDS             complement(1087..1692)
                     /locus_tag="VN377_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF996 domain-containing protein"
                     /protein_id="HWR27756.1"
                     /translation="MSKLGQAKVLGGIGALLSLLIFVPTIGFIISLVGLVLVFVAVKY
                     IAEETKDHAIFQNYLMNFIFDIIAIGAVIGIMIAVFGFAGGFSWFNNVQQPFTDFNSF
                     WESFGLVLVVGCALALVVAWVMLILGALYLRKSYNSIADHTKVDLFKTTGLVNFIGAV
                     TLIIVIGAFIIIIARILEIVSYFSLPENLPQTPEPPKTPTP"
     gene            1818..2267
                     /locus_tag="VN377_04905"
     CDS             1818..2267
                     /locus_tag="VN377_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27757.1"
                     /translation="MKKKIVVFCFFVLMVLMILPVVTSYCVVSERQPFMARVSEKQDS
                     DVQEKPVPMELIGYLLIHVSVYTPGEGLHPYVGANISVKGFFFSYNGQTDERGDCLFQ
                     VHTKLFRAKLYFIKVSIPPGDWLHKKLNSIYIQPQQIVYKDFLFVVL"
BASE COUNT          709 a          487 c          584 g          711 t
ORIGIN      
        1 atccagtgat ctgagcaatt ggcagatggg tagatcacat gcgcattcga aagatcagat
       61 ggcacccatg attgactggg atggtggaac ctggcgagag attgtccaat gctgtctgct
      121 ctttggagat cccgctcaac agctcagaac acctcatcca agtgaggcac catcgccacc
      181 ttcaaaacct gtcggtgaag atcttgggat ctggaacgta gaatattgct atactagcca
      241 aatgggcagc gaccctgaga atgatcaaat cttctgcctg ttcgactggg gtgacggaag
      301 caacaccgga tggcttggac ctttcaatgg cggagagacc gtcaccgcat gccatatctg
      361 gacagcgctt ggcacctatg aagtgaaagt aaaagcacga gacgtctggg gcgcgggaag
      421 cacctggtca gaatcattgg tcgtcactat cacggataat acaccgccag atgcccccga
      481 agtaaccggt ccttctgagg gaaaacctgg gaacccgtat ctcttcaata tgatcacctc
      541 cgatcctcag gatcagaaca tctattactt tgttgactgg ggagataaca catcgaccgg
      601 gtggctcgga ccgtatcttt ctggtactga gatccatcag acacattcct gggccgagaa
      661 gggaacctat accgtgaaag tcaaggcaaa agacatcatg gattcagaga gcgaatgggg
      721 cacagtatca attgtgatgc cgacggaata tacgttctca ctgttggggt tcatccagca
      781 cttactcggg atgttcccga acctgttccc agtgctacgg caccttgcag ggtattaaac
      841 ccctgcattc tctttttctt tttttctctt tttctttctc tttttcttgt tcatgatagt
      901 ataattatag tacatctaaa aaggcaatga atggagaagc agcacatcgc aaaacctcat
      961 gataaaagca acggtggtct gacgtaaaaa catccgattg gagtcctgaa aattttttga
     1021 ttagaattga tgctctttag tatgaaaaga aaaaaaatga agaaaagggg agaaggcggt
     1081 gtcgttttag ggtgttggtg tttttggagg ctctggtgtt tgtggtagat tttcagggag
     1141 cgagaagtat gagactatct caaggattct tgcgatgatg ataatgaacg ctccaatgac
     1201 gatgatgagg gtcacagcgc cgatgaaatt aaccaagcct gtggtcttga agagatctac
     1261 tttggtatgg tcagcgatgc tgttgtagct ttttctcagg tagagtgctc ctaaaatgag
     1321 cattacccac gcgacgacaa gagcaagggc gcatcctacg accagaacga ggccaaagga
     1381 ttcccaaaat gagttaaagt cagtgaaagg ttgttgtacg ttgttgaacc atgaaaagcc
     1441 gccagcgaat ccaaatacag caatcatgat gccgatgacg gcacctatcg cgataatatc
     1501 gaatatgaaa ttcattaggt agttttggaa tatggcatgg tcttttgttt cttctgcaat
     1561 atatttaact gctacaaaca caagcaccag cccaacgaga cttatgatga atccgatggt
     1621 tgggacaaat attaagagcg ataacagtgc tcctattccg cctaatactt ttgcttgacc
     1681 taattttgac atagtttttt ttctcctcta tggtaaatgt tgattcagaa acaactgaaa
     1741 gaacttaaat attacgctcc atagaggtag gtttttatac aagtaattat tataaatgtg
     1801 cagattgggt gataagtatg aagaaaaaaa tagtggtatt ttgttttttt gtgttaatgg
     1861 tgttgatgat tctaccggtg gtcacatcgt attgtgtggt ctcagagcga caacccttta
     1921 tggcccgggt ttctgaaaag caagattctg atgtgcagga aaaaccagtg cctatggagt
     1981 tgattggata tctgctcatc catgtctcgg tctatactcc tggcgagggt ttgcatccgt
     2041 atgtaggagc caatatcagt gtgaaaggat ttttcttttc gtataacgga caaaccgatg
     2101 agcgggggga ttgtttgttc caagttcata ccaagctttt cagagcgaaa ctgtatttca
     2161 tcaaagtgag tattcctcca ggagattggt tacataagaa actaaattct atctatatac
     2221 aaccacaaca aatcgtgtat aaggattttc tttttgtagt attatagtgt ctctcagatg
     2281 atcaaattct ttgagcggaa gaattaggat tctgggcttg ttattttttt tttatttgtc
     2341 cagcatcgaa atgggaaaaa gaaatttctt gtaccaaatt gttgtttttt tgtaaattgt
     2401 aaaagaaatg caccaatagt ttcgtttcgt aatcaccata aaaggtgggt ttggagtatc
     2461 tgccacaatt acgaacactg ggaccactga c
//
LOCUS       DATMXJ010000187         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_61563, whole genome shotgun sequence.
ACCESSION   DATMXJ010000187 DATMXJ010000000
VERSION     DATMXJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61563"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VN377_04910"
     CDS             <1..335
                     /locus_tag="VN377_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HWR27758.1"
                     /translation="GRGSGLRGGRGNAGLHKHKFMHMLKYMPDHFGVHGFKRDPSLIK
                     DDRIINVGQLDEKFHGKKEIDLSKEGFDKLLGGGLVEHGYKIKVKSASQKAIDKIAEL
                     GGEVKVEE"
     gene            347..1894
                     /gene="secY"
                     /locus_tag="VN377_04915"
     CDS             347..1894
                     /gene="secY"
                     /locus_tag="VN377_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWR27759.1"
                     /translation="MAEEKKSRLYALKPIIERWPAVQKPEVHVNFRSKMFWTVLCLIL
                     YFILTNIMIYGVSGQVLDMFASYRAIMAGASGSIMHLGIGPIVTASIILQLFVGAKII
                     KLDLTKKEDKAIYQGSQKVLVIAMIFIEAIPQVYGYLSPSEGLIGMVGGRGVADLIII
                     LQLFMGAYLVFLMDELISKWGIGSGISLFIAAGVSQSIFTGLFNWLPTQSTQPISISN
                     PPAGTIPGTYYLASQMNLGEMVDGGFQILFIGHGGFQNALVPLIGTIAIFLIVTFTES
                     SRIELPLAHAKVRGARGRYPIRLIYASNIPVILMSALLANINMFALLFYTHPGLKGLP
                     GIGGQWWIGTYNTAGGSTMPVAGGAWYLSNLNGVNSWLIPILSGGYGGHAPWQFAIKT
                     LIYLTIMIFGSVLFAKFWIETTNMGPTDVAKQIQQSGMVMPGFRRDPRVLGRVLERYI
                     PVVTVIGGAAVGALAAGADAIGTVGNASGTGVLLTVGIMIQLYEQIAKEQAMEMHPIL
                     RGFFGAE"
     gene            1906..2454
                     /locus_tag="VN377_04920"
     CDS             1906..2454
                     /locus_tag="VN377_04920"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWR27760.1"
                     /translation="MGVIVVTGIPGVGKTTVMQKAAEGLDITFVTFGSVMSDIAIDLK
                     LVKDRDEMRKLSLDQQKQLQIKTAEKVGKMKNVIVDTHCTVKTPKGYMPGLPEWVLRK
                     LNPTAIIIVEADPQEIYNRRAKDSTRNRDPDSVEKIAEHQMINRAAAMSYATLTGATV
                     KIVFNHDNALDEAVKDAEPVIR"
     gene            2451..3116
                     /locus_tag="VN377_04925"
     CDS             2451..3116
                     /locus_tag="VN377_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HWR27761.1"
                     /translation="MRKLDDVAQVGQMLLLMLLLFVMILIFGDANIRTLVALSLNSAF
                     APLIGFGGANPLLTIVLAGIIVVFLSSLFTHLFTNWKAMGQAQEASKAFNKEITKARK
                     EGNTNRLQKLMKMQPQIMKMTTQSSGGMMKSMFFLFLFIAPIFIWLTYFLGNVNYFYF
                     TVPWGNGVSLFGRNSWIMSNWFFLYMIFSYLAGSLIRQGLKWVSWSNWWQNIRKNIRP
                     SAK"
     gene            3124..>3377
                     /locus_tag="VN377_04930"
     CDS             3124..>3377
                     /locus_tag="VN377_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR27762.1"
                     /translation="MTTITISGLPGTGKTTVAKLLEQRLGLRYVYSGEIFRQFAQDHH
                     MSLEDFGNYCESHRKIDEELDRHQLDILKEGDVILEGRLSG"
BASE COUNT          951 a          736 c          817 g          873 t
ORIGIN      
        1 gtggccgtgg ctcaggatta cgaggtggta gaggcaatgc aggccttcat aaacacaagt
       61 tcatgcacat gctgaaatac atgcctgatc atttcggtgt tcatggattt aaacgagacc
      121 ccagtcttat caaagacgac cggatcatca atgtcggcca gctggacgaa aagttccatg
      181 gcaagaaaga aatcgatctc tccaaagaag gatttgacaa actcttaggt ggaggactcg
      241 tcgaacacgg gtataaaatt aaagtgaaat ccgcatctca gaaagcaatc gataaaattg
      301 cagaactcgg tggagaagta aaagtagaag aataaaggtt tttactatgg cagaagagaa
      361 gaagagccgg ttatatgcct taaaaccaat cattgaacgc tggcccgcag tgcaaaagcc
      421 agaggtccac gtgaatttcc gatcaaagat gttctggacc gttctttgtc tgatactcta
      481 ttttattctc accaatatca tgatctatgg agtgagcgga caagtactcg atatgtttgc
      541 cagttatcga gcgattatgg ccggggcatc tggttctatt atgcacctag gtataggacc
      601 aatcgtcacc gcatcaatta ttcttcaact cttcgtcggt gcaaagatca ttaaactcga
      661 tttgacaaag aaagaggata aagcgattta ccaaggaagc cagaaagtct tggtcatcgc
      721 gatgatcttc atagaagcca tccctcaggt ctatgggtat ctgagtccaa gtgaaggatt
      781 aattgggatg gtgggtggca gaggcgtagc ggatctgatt attatcttac agttattcat
      841 gggagcatat cttgtattcc tcatggatga actgatctcc aaatggggga ttggttctgg
      901 tatctccttg ttcattgctg ccggtgtttc acaatccata ttcaccggac tgtttaactg
      961 gttaccaacg cagtcaactc aaccaatcag catctcaaac ccaccagcag ggaccatccc
     1021 ggggacgtac tatctcgcat cccagatgaa ccttggtgag atggtagacg gtggattcca
     1081 gatactgttt atcggtcatg gtgggttcca gaatgcgctt gttcctttaa tcggaacaat
     1141 tgcgatattc cttattgtaa cgttcacaga gtcttcacgc attgaattac ctcttgccca
     1201 tgcgaaggtg cgtggtgctc gaggacgata tccgattcga ctcatctacg cttcgaacat
     1261 cccggtcatt cttatgtctg cactgttagc aaatatcaac atgttcgcat tattgtttta
     1321 tacccaccca ggattgaagg gtttacctgg aatcggaggt caatggtgga ttggaacgta
     1381 taatactgct ggcgggtcga ccatgccggt tgcaggagga gcgtggtatc tctcaaacct
     1441 caatggtgtg aacagctggt tgattccaat tcttagtggt gggtatggtg gtcatgcgcc
     1501 atggcaattc gcgataaaaa ccctcattta tcttacgata atgatctttg ggtcagtctt
     1561 gtttgcgaaa ttctggattg agacaaccaa tatgggaccg actgatgtgg caaaacaaat
     1621 ccaacaatca gggatggtca tgccaggatt caggcgagac cctcgcgtgt taggaagagt
     1681 actcgagcga tacatcccag tcgttactgt gattggtggt gccgctgtcg gtgccctcgc
     1741 agcaggtgct gatgctattg gtactgtcgg taacgccagt ggaacaggag ttcttctaac
     1801 ggtgggaatc atgattcaac tgtatgagca aatcgcgaaa gaacaagcaa tggaaatgca
     1861 tccgattcta agaggattct tcggagcaga gtagtgaggc agacaatggg agtaattgtc
     1921 gtaaccggga tacccggggt ggggaaaacc acagtgatgc aaaaagcagc agagggatta
     1981 gatatcacct ttgttacctt cgggtctgtc atgagtgata tcgccataga ccttaaatta
     2041 gtaaaagatc gcgacgagat gagaaaactc agcctcgatc aacaaaaaca acttcagata
     2101 aaaaccgctg agaaagtcgg taaaatgaaa aacgtcatcg tcgataccca ttgcacggtc
     2161 aaaaccccta aaggatacat gccgggatta cctgagtggg tcttacgaaa attaaaccca
     2221 accgcaatca tcatcgtcga agcagatcca caggaaatct acaaccgacg ggcgaaggat
     2281 tcgacacgga accgggatcc tgattctgtc gaaaaaatcg cagaacatca gatgatcaac
     2341 cgagctgccg ctatgtcgta tgcgacactg actggtgcga ctgtgaaaat cgtctttaac
     2401 catgacaatg cacttgatga ggcagtaaaa gacgctgagc ctgtgatacg atgagaaaac
     2461 ttgatgatgt cgcccaagta gggcagatgc tgcttctcat gcttcttctc ttcgtcatga
     2521 ttttaatctt tggtgatgca aatatccgaa cccttgttgc actctctctt aattctgcgt
     2581 ttgccccgct gataggtttc ggtggagcaa acccgctact caccatcgtg cttgctggaa
     2641 tcatcgtcgt gttcctctcc tccctcttca cccatttgtt cacaaattgg aaagcaatgg
     2701 ggcaggcgca ggaggcaagc aaggcattca acaaagaaat caccaaagca agaaaagaag
     2761 gcaacaccaa tcgcttgcag aaactcatga agatgcagcc gcaaatcatg aaaatgacaa
     2821 cacaatcatc tggtggaatg atgaaatcga tgttcttttt atttctcttc atagccccga
     2881 ttttcatctg gctgacgtat tttttaggta acgtaaatta tttttatttc acggtccctt
     2941 ggggtaacgg cgtgtcttta tttggtagga atagctggat tatgtcaaac tggtttttcc
     3001 tgtacatgat attctcctat ttggcaggtt cactcattcg acaggggtta aaatgggtca
     3061 gctggtcgaa ctggtggcag aatatacgga aaaacatccg accgtctgcg aaataggagc
     3121 atcatgacga cgatcacgat tagtggtctt ccaggaaccg ggaaaacgac tgtcgcaaaa
     3181 cttttagaac aacgccttgg gttacgatac gtctattcag gcgagatctt ccgacaattc
     3241 gcacaggacc atcacatgtc tcttgaggat tttgggaatt actgcgagtc ccatcgaaaa
     3301 atcgacgagg agctcgaccg ccatcaactt gatattctaa aggagggcga tgtcatcctt
     3361 gaggggcggc tctcagg
//
LOCUS       DATMXJ010000188         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_45849, whole genome shotgun sequence.
ACCESSION   DATMXJ010000188 DATMXJ010000000
VERSION     DATMXJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45849"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VN377_04935"
     CDS             <1..847
                     /locus_tag="VN377_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWR27763.1"
                     /translation="NGVRAIDLTNKETLQKLLGRILPLKQKLIDIYDIKTTLDGKEIL
                     EDYYAYGKRLQEYITITHIELNKAIQKKKNILLEGAQGTMLDVEFGTYPYTTSSHVIA
                     GGAAIGAGIGPRVIDEIIGIVKAYTTRVGEGPMPTELLDKTGEHLQKVGHEIGTTTSR
                     PRRCGWLDLVVLKHSCMISGITKLAITKLDVLNVLLEIKVCTHYTLNGKNIDYFPARI
                     EDVASCKPIYKTFKGWTTIDPSSAKFSDLPKEAQTYLRFIEKETEVPISLVSIGPGRT
                     ETIEV"
     gene            complement(844..1083)
                     /locus_tag="VN377_04940"
     CDS             complement(844..1083)
                     /locus_tag="VN377_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HWR27764.1"
                     /translation="MTNKASFLFFFPYAKDASVIAQSLSPEIKHKIPKSTVTFSVDEK
                     TLTMTIESKDLSSLRAACNSYLRWIQTALSVKQLV"
     gene            complement(1080..1220)
                     /locus_tag="VN377_04945"
     CDS             complement(1080..1220)
                     /locus_tag="VN377_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HWR27765.1"
                     /translation="MTRYKCGLCKKAVQFDVKNIGMQCPYCGSKVFYKERPTVAKRIK
                     SV"
     gene            complement(1217..1510)
                     /gene="rpl37A"
                     /locus_tag="VN377_04950"
     CDS             complement(1217..1510)
                     /gene="rpl37A"
                     /locus_tag="VN377_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37Ae"
                     /protein_id="HWR27766.1"
                     /translation="MSKRTKKVGTSGRFAERYGVKSRTMIREVESQQRLRHACPRCGQ
                     KAVQRKSTSIWECSKCGNVFAGGAYLPTTPSGVTAERILKGLPAEMPVEEESK"
     gene            complement(1513..2412)
                     /locus_tag="VN377_04955"
     CDS             complement(1513..2412)
                     /locus_tag="VN377_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HWR27767.1"
                     /translation="MTIVPIIKRDYITKLAEQGKRIDGRGFDQYRNIEIETKVVNKAE
                     GSARVKIGNTQVLCGIKMDIGEPFPDTPKTGVMSTAAEFVPLASPDFESGPPREDAIE
                     LARVVDRGVRESQVIQLEKLCVTPGEKVWLVFIDIHILDYDGNLFDAASLAALAALLT
                     TKVPVSRFLKELNENDRKSWQEKLQDLYTIPSINEVAFGTDEANKNPNTIEDFPLPMI
                     EPPISCTAVKINDFVLFDPCFDEEIIADVRLTVATDHKGDIRAMQKGLSGSFTKEEIQ
                     KVIKGALDNGQEIRKKLNQSIGK"
     gene            complement(2409..3143)
                     /gene="rrp41"
                     /locus_tag="VN377_04960"
     CDS             complement(2409..3143)
                     /gene="rrp41"
                     /locus_tag="VN377_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HWR27768.1"
                     /translation="MKSDIELINSEGRRLDGRGPHDIRKIKIEAGVLHRADGSCSLEW
                     GGNKVLAAVYGPREAVPRHTQNPLKAIVQARYNMAAFSVEDRKRPGPDRRSVEISKVI
                     SEALEKVILIEQFPRASIDVNIEVLDAEAGTRCAGLTAAGVALVDAGIPMRDIPVACA
                     AGKIGGRVVLDLGKEEDNFGDADLPIAISPRTGEVLLLQMDGHLTVDEFNEAFDLAVK
                     GCHLVSELQKKAIIQKYQSVAAEVQE"
     gene            complement(3143..3823)
                     /gene="rrp4"
                     /locus_tag="VN377_04965"
     CDS             complement(3143..3823)
                     /gene="rrp4"
                     /locus_tag="VN377_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HWR27769.1"
                     /translation="MSNEGRQVVIPGQILGETPALRPGRGTFVENKKILAERLGVLNI
                     QDNFVHIIPLKGRYDAVVGDFVIGVVEEASTSNWMVDINAPYPALLHVHEVPWEIEFA
                     DTEKYLNTGDTILAKVLSVDESKKLQITLNDRNLFKLKGGHLIEFEPSVYRRIIGKKY
                     SMIEIIQKYTRCRIKVGKNGRIWIDGDADGIEKVLAAISMIEREAITFGLTDRIEEFL
                     KKDAKDVR"
     gene            complement(3851..4546)
                     /locus_tag="VN377_04970"
     CDS             complement(3851..4546)
                     /locus_tag="VN377_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HWR27770.1"
                     /translation="MVSLENAVIARLHKGADRFEILVDPHETENIMEGKTENILSALA
                     IDAVFSDSKKGTHAPIESLQKHFNTTDISVIAKEIMLKGEIQLTTEQRREMQEKKRKR
                     IVDLIVKNAMDPQTKTPHPRTRIELALDEAKVHIDPFKSVNQQMKTILEHLRPILPIS
                     MDQARISVKIPPEHVGKAYGAVRSFGTLEREEWQSDGTWIGIVKLPAGMQTDFYDRLN
                     TLTKGNVETRVLK"
     gene            complement(4549..>5173)
                     /locus_tag="VN377_04975"
     CDS             complement(4549..>5173)
                     /locus_tag="VN377_04975"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HWR27771.1"
                     /translation="GTTTVGVKFRDGAILIVDKRITSRLIEPDSIEKIFIIDDHIGCA
                     TSGLVADARSLIDRARLDAQINEITYSEKIQVQTLVKRICDFKQTYTQYGGVRPFGTA
                     LLIAGVDQTGARLFSTDPSGALMEYKAASEGAGRTGAMAYFEEHYKENVSMEEAIDLG
                     VKALHKGTEGKLNPDAIEIGVVKKGVKFHILSAAETKTFVTKAIGGK"
BASE COUNT         1325 a         1248 c         1077 g         1523 t
ORIGIN      
        1 caacggcgtc agagcaattg atcttaccaa caaagaaact ttacagaaat tacttggcag
       61 aattcttccc cttaaacaaa aattaatcga catctatgat atcaagacga cacttgatgg
      121 aaaagaaatc ctggaggact actacgcata cgggaaacga ttacaagaat acatcaccat
      181 aacccatatt gaactgaaca aggcaatcca gaaaaagaaa aacattcttt tagagggtgc
      241 ccaggggact atgcttgatg ttgagttcgg cacctatccg tatacgacat cgtcacatgt
      301 cattgctggt ggtgcagcaa ttggcgcagg gatcggacca cgagttatcg atgagatcat
      361 cggtatcgtc aaagcataca caacgcgagt gggggaagga cctatgccaa ccgaacttct
      421 tgataagaca ggggagcatt tgcaaaaagt tggtcatgaa atcgggacga caacaagcag
      481 accacgacga tgtggctggc ttgatctcgt cgttctaaaa cattcttgca tgatatcggg
      541 gattacaaaa cttgcgatca ccaaacttga tgtcttaaat gtacttctgg aaatcaaggt
      601 ttgtacgcat tatactctca atgggaagaa tatcgattat ttccctgctc gcattgaaga
      661 cgtggcttcc tgcaaaccaa tatataagac attcaaaggt tggacgacta tagatccgtc
      721 ttctgcaaaa ttctccgatc tgccaaaaga agcacaaaca tatctccgat tcatcgaaaa
      781 agagacagaa gttcccatta gtcttgtctc aatcggacca ggacgaaccg aaaccatcga
      841 ggtctaaaca agttgtttta ctgacaatgc cgtctgtatc caccgaagat acgagttaca
      901 cgctgctcgt agggagctga gatccttaga ctcgatggtc atcgtcagtg tcttctcatc
      961 gacagaaaac gtgacagttg attttggaat cttatgtttt atctcaggag atagtgactg
     1021 agcaatgaca gaggcatcct tagcatatgg gaagaaaaaa aggaatgatg ccttgttcgt
     1081 catacacttt taatgcgttt ggctaccgtt ggtcgttctt tgtagaacac cttgcttccg
     1141 cagtacggac attgcattcc gatattcttc acatcgaact ggactgcttt cttgcacaaa
     1201 ccgcatttat accgggtcac ttggattcct cctcaacagg catctcagca gggagccctt
     1261 tgaggatcct ctcagcggta acaccagatg gtgtcgtggg taggtacgcc ccgccagcaa
     1321 agacattacc gcatttcgaa cattcccaga tactggtgct tttgcgttgt accgctttct
     1381 gaccgcacct ggggcaggca tgacggagtc gttgttggct ttcgacttcc cggatcatgg
     1441 ttcgtgattt cacaccgtat cgttctgcga agcgaccgga ggtgccgact ttctttgttc
     1501 ttttggacat atttatttcc ctatactttg gttaagtttc ttccgtattt cttgcccgtt
     1561 atcaagagct ccttttataa ctttttgtat ctcttctttg gtaaagctcc ctgaaagccc
     1621 tttctgcatt gcgcggatat cacctttatg gtcggtagcg actgttaatc gaacgtcagc
     1681 gatgatttcc tcgtcaaaac acgggtcaaa gagcacaaaa tcattgattt tcacggcggt
     1741 acatgatatt ggaggctcaa tcatcggcaa aggaaaatct tctatggtat ttggattttt
     1801 atttgcttcg tcggttccaa atgcgacctc gtttatacta ggaattgtat ataaatcttg
     1861 aagtttttcc tgccaagatt ttctatcgtt ttcgtttaac tcttttaaaa atcgagaaac
     1921 gggaaccttg gtcgtcaata acgcagcaag cgcagcaaga gatgctgcat caaacaaatt
     1981 cccatcataa tccaggatat gaatatcaat gaacaccagc caaacctttt ccccaggggt
     2041 gacacatagt ttttctagtt gtattacttg ggattctctg acacctctat caactactct
     2101 tgcaagctct atggcatcct ctcgaggtgg acctgattca aaatcaggag atgccagagg
     2161 aacgaattct gcagcggtac tcatgacgcc agtttttggg gtatccggga atggttcccc
     2221 aatgtccatt ttgataccac aaagtacctg agtattgccg attttcaccc gagcagagcc
     2281 ttcggccttg ttgacgactt tcgtctcaat ctcgatgttc cggtattggt caaagccacg
     2341 cccgtcgata cgtttgcctt gctcagcaag ctttgtgata taatcgcgtt tgatgatagg
     2401 gacaatggtc attcttgtac ctccgcggca acagattgat acttctgtat tatagccttc
     2461 ttctgaagtt ctgagaccaa atgacagcct ttcacggcaa gatcaaaagc ttcgttaaac
     2521 tcatcgaccg tgagatgacc atccatttgt aacaacaaca cttctccggt ccttggtgaa
     2581 atagctattg ggagatcagc atcgccaaag ttatcttctt ctttcccaag atctaggacc
     2641 accctgccac cgattttccc tgcagcacaa gcgacaggga tatcacgcat gggaatccct
     2701 gcatctacca acgcgacacc agcagcggta agtcctgcgc atcgagttcc tgcctcggca
     2761 tccagcacct cgatgttgac atcgatactt gcgcgaggga actgctcaat cagaataact
     2821 ttttccaacg cctcagagat aacttttgaa atctcaacgc ttcggcggtc tggtcctgga
     2881 cgtttacggt cttcaacact aaatgctgcc atattatatc gtgcttgcac aatcgctttt
     2941 aggggattct gcgtgtgtcg cggaacagct tcccgggggc catagacagc agcaaggact
     3001 ttgttaccgc cccattccag ggaacaagag ccgtctgctc gatgaagcac gcctgcttcg
     3061 atttttattt tccgtatatc gtgtgggcct cgcccatcta atcgtcttcc ttcactgttt
     3121 attaactcaa tatctgattt cattatctta catcctttgc atcttttttc aagaactctt
     3181 ctatcctatc ggttagccca aatgtgatgg cttctcgttc tatcatagag attgcagcta
     3241 acaccttttc tatgccgtct gcgtctccat caatccaaat acgcccgttt ttaccaacct
     3301 taatgcgaca acgggtgtat ttttgtataa tctctatcat cgaatatttc tttcctatga
     3361 tgcgacggta caccgatggt tcgaattcta tcagatgacc gcccttcaat ttgaacagat
     3421 tgcgatcgtt gagtgttatc tgaagtttct ttgattcatc aacggaaaga actttcgcca
     3481 gtatggtatc gcctgtgttt aaatattttt ctgtatcggc aaattcaatc tcccatggca
     3541 cctcatggac atgcaataaa gcaggatatg gagcgttgat gtcgaccatc cagttcgagg
     3601 tcgacgcttc ttcaacaaca ccgatgacaa aatctccgac caccgcgtca taacgcccct
     3661 ttaaaggtat gatatggacg aagttatctt gaatgttcag gactccgagt cgttccgcaa
     3721 ggattttttt gttttctaca aatgtcccac gaccaggcct gagagcaggt gtttcaccca
     3781 gaatctgacc cggaataact acttgacgtc cttcatttga cataaaatcc agtatccttc
     3841 tttcataaac ctatttcaaa actcttgttt caacgtttcc ctttgtcagt gtattgagcc
     3901 gatcataaaa atctgtttgc ataccagctg gtaacttcac gattcctatc catgtcccgt
     3961 ccgattgcca ctcctcccgt tcaagtgtac caaagctccg tacagcacca taggctttcc
     4021 cgacatgctc tggtgggatt ttcacagaga tccgtgcctg atccatcgag atcggtagaa
     4081 taggccgaag gtgctcaagg atggttttca tctgttggtt caccgattta aacggatcaa
     4141 tatgtacttt tgcttcatcc aaagcaagct cgatacgggt tctcggatgt ggcgtctttg
     4201 tttgagggtc cattgcgttc tttacaatta aatcgacaat tcgtttcctt ttcttctcct
     4261 gcatctctcg acgctgttct gtggtaagtt ggatttctcc ctttaacatg atttctttgg
     4321 cgattacgga gatatcagtc gtgttgaaat gtttctgaag ggattcaatt ggagcatggg
     4381 tacccttctt tgaatcgctg aacaccgcgt caatcgctaa agctgagagg atattttcag
     4441 tttttccctc catgatgttc tctgtctcat gaggatcgac gaggatttcg aagcgatctg
     4501 ctcctttatg taaccgggca atcactgcgt tttctaaact gaccaacttt atttacctcc
     4561 gatagctttg gttacaaagg tctttgtttc tgcggctgag aggatatgaa acttgactcc
     4621 ttttttcacg acaccgatct cgatagcatc aggatttagc tttccttcag ttcctttgtg
     4681 gagtgctttt acacctaggt caatcgcttc ttccatggaa acgttttctt tatagtgttc
     4741 ctcgaagtac gccatggccc cggtacgtcc tgctccttca ctggccgctt tatattccat
     4801 aagagcacca gaaggatcag tggaaaacag tcgtgcaccg gtctgatcga caccagcaat
     4861 caacaatgca gtgccaaaag gtcttactcc accgtactgt gtgtaggtct gtttaaagtc
     4921 acaaatccgt ttcacgaggg tctgaacctg aattttctcg ctatatgtta tttcattaat
     4981 ctgcgcgtct agtcgagcgc ggtcaatcaa ggaacgagca tcggcaacaa gaccagaagt
     5041 cgcgcacccg atgtgatcgt caataatgaa aatcttctca atggaatccg gctcaatgag
     5101 tcgagatgta atgcgtttat caacaattaa gatagcgcca tctcggaatt tcactcccac
     5161 tgttgttgtt cca
//
LOCUS       DATMXJ010000189         7056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_29179, whole genome shotgun sequence.
ACCESSION   DATMXJ010000189 DATMXJ010000000
VERSION     DATMXJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29179"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /locus_tag="VN377_04980"
     CDS             <1..2240
                     /locus_tag="VN377_04980"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWR27772.1"
                     /translation="IETVPASADTSCGYETTAPSSSVNAITPYWKNTGATLSATASDS
                     TSGVKDVTLLYRFSSDNVSWDGWVSAGVDTAMPWSWSFIFVNGSGYYQFDSIAQDHAA
                     NTQSTPGGADAWAGFENQAPSSVITVIPDYWISSSPLVITATVGDTGPSGLKNVTLFY
                     RYRTTNTSSWGSNISFGVDTIPWDLTSWAFSFPEGAGHYQFYSIATDNATNIETASGG
                     NGDATCGFNTGAPLSEVNEITPYWSSTIPLLIEASAEDFGPSGLKNVTLYYYNSTDNS
                     TWAGPWRYGVDTDPWVDCSWDFLCPNQTGYYRFYSCASDNSSHVENAALINDTGCGYD
                     VQPPACSISYNRTKDSFKAYDSLGIHASFTEPYSGVDESSVLIAISTMGNGSLVNASM
                     NMIDNTHWSYDWVIPSGSDEDGPFTIRIYANDMASNPLDPYPTIDTTKQIDNTPPVIS
                     NLSMSNVTTGSVIISWLTNENATSHIEYGPTPSYGSWFISSPFIISHRCTLSGLSAAT
                     TYYYQVISYDSAGNQDTSLNGSFTTSAQTSKRRDIVQKNINTPPSNPIIDGPTTGRIS
                     QIYMYTVSSHDANNDTITYIFNWDDGSIDTSELIPTGIQCIKNHSWTKAGKYTIHVTA
                     SDRTTSSSSEMTVWIDAIPVDDVGYFLDDDSDGIFDVFHNNVTGLETVMEMRNGVYLI
                     DVNGDQRWDYEYNATSGTLMSILQQRPITEETPFPLLLLCCLIFVVVLLLLVIVYRRR
                     FNKKQ"
     gene            complement(2199..3755)
                     /locus_tag="VN377_04985"
     CDS             complement(2199..3755)
                     /locus_tag="VN377_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27773.1"
                     /translation="MTPAATAGDYSLLLKVRDPSRPGLQVLCRVPKGTLYTYYHPWTG
                     TAWDFIVNHTYIGVATQGDTLPNIVKAGMVLTDAGLAFGDADTLSKWINPTKNAWDDF
                     DWIRYSCQTADNEDQATALLTTDAVDQLHATGVSENLFVVGPQKAVVIEADALRYTIQ
                     DIPDVLVMSNYAKNLWRTQQIRSLPIASSFEAQKETWVRRGTIVHLGSLCGITIRAIT
                     PSVITAQVFPVFAFKNSELYKEVTISLGDHATVGPYSVKLFEINGNKARISLSNAMYS
                     WEQELQEQIQPMIGNITVQDLIRWSRLHAADLDGLRPMCEDQNTYEAAMIYKIPEEHA
                     SLLSSGWFAANHACSSIYIPIHICDDDFYDPYETGEAAALSLELLQKYGHGNVTSLCQ
                     SVENVFLFENELNEALAHLMIHADINISSFLTDLDNGMQEQAFLTEQLWLSAPNLSQG
                     IVQDIWKGNYMVTQQNIQKAVSLLIGIPGSDTSISLLEKISQNISELTDKRQAMESLF
                     FVESSAVHYY"
     gene            complement(3906..4790)
                     /locus_tag="VN377_04990"
     CDS             complement(3906..4790)
                     /locus_tag="VN377_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HWR27774.1"
                     /translation="MAKARARTTTRKVKDRWKAKNWYTILAPSLFNNVPVAETLADSP
                     ESLIGRVTEVSLQDITNDFRKSHIKLLFSIDKIEQNTAHTQLKGHLLTSDYLRRMIRR
                     RKSRVDGVFDVETRDGALLRVKPFAIAEKRIQSSQKKLIRNVMHDVITKEGKAKTLNS
                     FIKDCLDGKIGSEIYKHCKIYYPVKRIEVNKTEIIRLPTVIIEDEKPVEKPEEQPREP
                     SKEESEVPSEEEITEEKPETEIPVEEKEEPVEEELAAEDLEKVEKPKKKTKAKAVTKK
                     KTTTKKTAKKKKEDAPES"
     gene            complement(4914..5711)
                     /locus_tag="VN377_04995"
     CDS             complement(4914..5711)
                     /locus_tag="VN377_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27775.1"
                     /translation="MTVEEIECKTALSTSTLPGLTYSLNPYRGCQHNCAYCYAPNVLR
                     VPREHWADDLKVKKNIPLVLAQELKKKKPGVVGISTVTDPYQPLEEKYKLTRFCLEQL
                     LQYDFPVHIQTKSALVIRDIDLLVRFSDSQVMLSIGTLHDNDRRLLEPHTSSIQERIT
                     ALQKCAKAGLKTAVFFGPVYLTTSVNEIPGFLDIMKDAGVQEIWVDMLRLKPGVMENI
                     QKTLTLDGEIAQRFSKQMQKPMDHYRCIREEIRKQGKQRNLRILDAF"
     gene            complement(5713..6993)
                     /locus_tag="VN377_05000"
     CDS             complement(5713..6993)
                     /locus_tag="VN377_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HWR27776.1"
                     /translation="MQNVTKNQKKLLPNEKKKSTSVCALCRGTKFLCGKNRCPVIVRY
                     HAKVKNKPLLDSLRLEGASPPSVFIGRIGYPKVAIGPMIPPVMGDTSEIDTPERWLHK
                     TLDDIVDFRSQLVRGKFTVDVTNVESPHRIVEFTRELALSKNAVFADAVFEKKPYGKI
                     AFYDEVQPHGPSAPLRTLDITNPKYEQHIEKAYYDTDLKARDAILDIYKNGVLVSKIQ
                     RAFSVGAFGIKKNRRFVPTRWSITAVDSTLGADLMKKTKTYPFINEVRAYYLNQFDNR
                     WIILMMPSEWQYELIEAWYPNTTWNQYGTSISIFNSYEFYDGRTTYAEIGGCYYAARL
                     AVNELLSKERRQAGVVILREAHPGYIMPIGVWNVRESVRHALTQPCKKFNTVQESLVY
                     VSEIMDIPIQRWIRNSAVLKNQLYQRRLEDFLVR"
BASE COUNT         1784 a         1560 c         1568 g         2144 t
ORIGIN      
        1 atattgagac ggtccctgcc agtgcagata caagttgtgg ttatgaaacg actgccccga
       61 gttcttcagt taatgcgata acaccctatt ggaaaaacac aggggcgact cttagtgcta
      121 ccgcaagcga tagtacaagc ggggtaaaag atgtcacctt gttgtatcgg ttcagcagtg
      181 acaatgtttc atgggatgga tgggtaagtg ctggagtcga tactgcgatg ccctggtcat
      241 ggagttttat tttcgtgaat ggcagcgggt attatcagtt cgacagtatc gcgcaggatc
      301 atgcggcgaa tacacaatcg acccctggag gtgctgacgc ttgggcgggg tttgaaaatc
      361 aagcgccatc ctcagttatt actgtgattc ccgattattg gatatcttct tcaccgttgg
      421 taattactgc aacggtaggt gataccggac cgagcggatt gaagaatgtc acactctttt
      481 atcggtaccg aaccacaaac acatcaagtt ggggaagcaa catcagtttt ggtgttgaca
      541 ccatcccctg ggaccttact agttgggcct tctcattccc cgaaggagca ggtcattacc
      601 agttctatag tatcgcaaca gacaatgcaa cgaacatcga aacagcatct ggtggcaatg
      661 gagatgcgac ctgtgggttt aacactggtg ctccgttatc agaggtaaat gaaataaccc
      721 cctattggtc atccaccata ccgctgttga tagaggcaag cgcagaggat ttcggaccaa
      781 gtgggctgaa gaatgtgaca ttgtattatt ataacagtac tgataacagc acatgggctg
      841 gtccctggcg atatggtgtt gatacggatc catgggttga ttgcagttgg gatttcctgt
      901 gtcccaatca aaccgggtat tacagattct actcttgcgc ttcagacaat agtagtcatg
      961 tcgagaatgc tgcgctcatc aatgacaccg gatgtggata cgatgttcaa cctccggcat
     1021 gttcaatttc ctacaatagg actaaagatt ctttcaaagc ttatgattcg ctagggatcc
     1081 atgcaagctt tactgaacct tattcaggtg tagatgaatc cagcgttctt attgcgattt
     1141 ccacgatggg aaacggcagc ctagtcaatg cttcaatgaa catgattgat aacacccact
     1201 ggtcctatga ttgggtcatt ccatcaggtt cagatgagga cggtccgttc accataagga
     1261 tttatgcaaa cgacatggca tcaaaccccc tcgatccgta cccaaccata gatacaacaa
     1321 aacagattga caacacacct ccggtcattt ccaacctctc tatgagtaat gtcacgacag
     1381 gctctgtcat aatatcctgg ttaaccaatg aaaacgcgac aagtcatata gaatacgggc
     1441 ctactccgtc ctatggttcc tggttcatta gctctccctt catcatctcg cataggtgta
     1501 ctctatcagg cttatccgca gctactacct attattacca ggttatatcc tatgattctg
     1561 ctggcaatca agatacttcg ctgaacggta gttttacgac aagtgcacaa acatcaaaaa
     1621 ggagagacat tgttcagaaa aatatcaata cccccccttc gaacccgata atcgatggtc
     1681 caacaaccgg tcgtatttca cagatctata tgtatacggt gagttctcat gatgccaaca
     1741 atgacactat tacctatatc ttcaactggg atgatggttc catagataca agcgaactga
     1801 taccaacagg gatacagtgc ataaaaaatc acagttggac gaaagccgga aaatatacaa
     1861 tacatgtgac tgcgagtgat cgtacaacaa gttcatcatc agaaatgacc gtctggatag
     1921 atgctattcc tgttgacgat gttggttact ttttagatga tgatagcgat gggatattcg
     1981 atgtattcca taacaatgtg acagggttgg aaactgtaat ggaaatgagg aatggagtct
     2041 atctcatcga tgtgaacgga gatcaacggt gggattatga gtataatgca acaagcggta
     2101 ctctgatgtc gatactccaa caacgaccaa taactgaaga aacaccattt ccgttgctct
     2161 tactatgctg tctgattttc gtagtcgttt tattgctgtt agtaatagtg taccgcagac
     2221 gattcaacaa aaaacaatga ttccatcgcc tgtcttttat cagtcaattc agagatattc
     2281 tgagagattt tttcaagcaa agaaattgaa gtatctgaac ctgggatgcc gatgagcaac
     2341 gatacagctt tttgtatgtt ctgctgagtc accatgtaat ttcccttcca gatgtcttga
     2401 acgattcctt gtgaaaggtt tggtgcagac aaccagagtt gttccgttag aaacgcttgt
     2461 tcttgcatcc cgttatccaa gtcggttagg aacgaagaga tattgatatc agcatgaatc
     2521 atgagatggg caagtgcctc gtttaactca ttttcaaaaa ggaatacatt ttctacactt
     2581 tggcaaagtg acgtaacatt tccatgacca tatttctgaa ggagttcgag ggaaagagct
     2641 gctgcctctc ctgtttcgta ggggtcatag aaatcgtcat cacagatatg tatcggaatg
     2701 taaattgagg agcatgcatg gtttgcagca aaccagccgc tgctgaggag gcttgcgtgt
     2761 tcctctggta ttttgtatat catcgcggcc tcatatgtat tttggtcttc gcacatcggg
     2821 cggagtccgt caaggtctgc ggcatgcaac cgtgaccagc gtatcagatc ctggaccgtg
     2881 atgttcccaa tcattggttg gatctgttct tgtagttctt gttcccatga gtacatcgca
     2941 ttactcaatg atatcctggc tttgtttcca tttatctcaa aaagtttcac gctgtaaggt
     3001 ccgaccgtcg cgtgatctcc taacgagatg gtcacctctt tataaagttc ggaattttta
     3061 aatgcaaaga ccggaaacac ttgagcggtg atgacggatg gtgttatcgc tcgtatcgta
     3121 attccacaaa gagacccaag atggacaatc gtcccacgtc gtacccaagt ttctttctgt
     3181 gcttcaaatg atgatgcgat cggcagtgat ctgatttgtt gtgtccgcca gagatttttt
     3241 gcatagttgg acatgacaag tacatcaggg atgtcctgta tcgtatatcg gagtgcgtcc
     3301 gcttcgatca caactgcctt ctgaggtcca acgacaaata gattctcaga aaccccagtc
     3361 gcatggagtt ggtccactgc atctgtggtg agtaaagcgg ttgcctgatc ttcgttatca
     3421 gcggtctgac agctgtaccg aatccaatca aaatcgtccc atgcgttctt tgttgggttt
     3481 atccactttg aaagcgtgtc tgcatcacca aaggcgagac cagcatcggt aaggaccatc
     3541 cctgctttga caatatttgg aagtgtatca ccttgtgttg cgacaccaat gtaggtatgg
     3601 ttgacgatga aatcccatgc tgtcccagtc catggatgat aataggtgta tagtgttcct
     3661 tttgggactc ggcagagaac ctgcagcccg ggtctgcttg gatctcggac ttttaaaaga
     3721 agactgtaat ctcctgctgt ggcagcaggt gtcacgacga tatctttaca gctttttccg
     3781 attggtacga gtatgacgga gcataaaaag atgcatatga aaaaacatgg caggattttc
     3841 tgcttctgag gcatatagga ataaaatagt aaaccacgat atataaacgt atgctccgag
     3901 aacatttatg attcaggagc gtcttctttc tttttctttg ctgttttctt tgtcgtggtt
     3961 ttcttctttg ttactgcctt tgcttttgtc ttcttcttcg gtttttctac tttttccaaa
     4021 tcttcagcag caagctcttc ttccacgggt tcttcttttt cttcgactgg tatttcggtt
     4081 tctggttttt cttcagtgat ttcttcttca gatggtactt ctgattcttc tttgcttggc
     4141 tctcggggtt gttcttctgg tttctctaca ggtttttcat cctcgataat cacggttggt
     4201 aatcggatga tttctgtctt attcacttca atgcgtttca ctgggtaata aatcttacag
     4261 tgcttgtaaa tctcgctgcc tattttcccg tcaagacagt ccttgataaa ggaattcaat
     4321 gtttttgctt ttccttcttt ggtgatgaca tcatgcatga cgtttctgat aagtttcttt
     4381 tgtgaacttt gaatgcgttt ctcagcgata gcaaacggtt ttactcgcag aagtgcgccg
     4441 tcccttgtct cgacgtcaaa cacaccatcg acgcgagatt ttctccttcg aatcattcgc
     4501 cggaggtaat ctgatgtgag caggtgaccc ttcagttggg tgtgggccgt gttctgttcg
     4561 atcttatcga tgctaaacag gagttttata tgtgatttcc gaaaatcatt cgtgatgtcc
     4621 tggagggata cttcagtgac tctgccaatc aggctttctg gagagtctgc gagcgtctca
     4681 gcgacaggga cattgttgaa taatgatggt gcgagtatcg tgtaccagtt ttttgctttc
     4741 catcgatctt tgacttttct agttgtcgtg cgtgcacgtg ctttggccat atctatcact
     4801 tataaatatc gtaatttttt tattaaggga tttttgcagt aatagagttc ttatacttaa
     4861 atgctacgag ggtgtatccc ttggggaaaa ccctatatta ccacggaaag aggttaaaat
     4921 gcatcgagga ttctgaggtt tcgctgcttt ccctgttttc gtatttcttc cctgatacat
     4981 ctgtaatgat ccataggttt ttgcatttgt tttgaaaacc tctgagcgat ttccccatct
     5041 aatgtcaagg ttttctggat attttccatc acaccaggtt tcagacgcaa catatcaacc
     5101 catatttctt gaacacctgc gtctttcatg atatcaagga atccagggat ctcattcacc
     5161 gatgttgtca ggtataccgg gccgaagaat acagcggttt tcagacccgc ttttgcgcat
     5221 ttctgtaatg cagttattcg ttcttgaatc gaggatgtgt gtggttcaag aagacggcga
     5281 tcattgtcgt gtaatgttcc gatactgagc atcacctgag aatcagaaaa tcgtacgaga
     5341 agatcgatat ctcgtatgac tagagctgat tttgtctgga tgtgaactgg gaagtcatac
     5401 tgcaataatt gttctaaaca aaaccgggtc agcttatatt tttcctccaa tggttgatac
     5461 gggtcagtca ctgtcgagat tcctacgacg cctggtttct tttttttcaa ctcctgggct
     5521 aaaaccagtg gaatattttt ttttaccttg aggtcgtccg cccagtgttc ccgcggtact
     5581 cgtaggacgt ttggtgcata acaatacgcg caattatgtt gacagcctcg gtatgggttc
     5641 agggaatagg tcaaccccgg taatgtcgat gtggacaacg cggttttaca ttcaatttct
     5701 tccacggtca tgttacctca ctaggaaatc ctccaatcgt cgttgataca gttggttctt
     5761 cagtaccgcg ctgttccgta tccatcgttg gataggaata tccataatct cggagacgta
     5821 cactagtgac tcctggactg tattgaactt tttacacggc tgggtcaacg catgacgaac
     5881 gctttcccgc acgttccaca caccaatcgg catgatatac ccaggatgtg cctcccggag
     5941 gatcacgact cctgcctgtc ttcgttcttt gctcaaaagc tcgttgaccg caagcctagc
     6001 agcataataa cacccaccga tctcagcata ggtcgtccga ccgtcataga attcatagct
     6061 gttgaatatg gagatactgg tcccatactg gttccatgtc gtattcgggt accatgcttc
     6121 aataagctca tattgccatt ccgagggcat catcagaatg atccatcgat tatcaaattg
     6181 attcagatag tacgcacgga cctcgttgat gaacgggtag gtctttgttt ttttcatcaa
     6241 atccgcacca agcgttgaat ccactgcggt gatgctccac ctggttggga caaaacgtct
     6301 gttctttttt atcccaaatg ctccaacact gaacgcacgt tgaatctttg agaccaacac
     6361 cccattttta tagatatcaa gaattgcatc cctggctttc agatcagtat cataataagc
     6421 tttttcgatg tgctgctcat atttggggtt tgtgatatcc aatgttctga gaggagcgga
     6481 cggtccgtgt ggctggactt cgtcatagaa cgctatcttc ccataaggtt ttttttcaaa
     6541 gactgcatca gcgaacaccg cgttcttaga gagagctagc tccctggtga attccacgat
     6601 gcgatgggga ctttcgacat tggtcacatc aacagtgaat ttccctctga cgagctgact
     6661 acgaaaatcc acgatgtcat ccagtgtttt atgaagccat cgttcagggg tgtcaatttc
     6721 cgaggtgtct cccatcactg gtgggatcat tgggccgatg gccacctttg gatatccgat
     6781 ccgtccaata aaaacactcg gaggagaggc accctcgagt cgtaaactat caagtaatgg
     6841 tttatttttt accttggcgt gatatcggac tatcaccggg catcggtttt tcccacataa
     6901 aaacttagtt cctcgacaaa gagcacagac gcttgttgat ttctttttct catttggaag
     6961 aagttttttc tgattctttg tgacgttctg caccagatcc cccatgattc aagcgctctt
     7021 ttataccttg ataagagagc attgaaagta tataat
//
LOCUS       DATMXJ010000190         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_71227, whole genome shotgun sequence.
ACCESSION   DATMXJ010000190 DATMXJ010000000
VERSION     DATMXJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71227"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..332)
                     /locus_tag="VN377_05005"
     CDS             complement(75..332)
                     /locus_tag="VN377_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27777.1"
                     /translation="MKPSTYASLVTLVFLTHQNEICKSIESAIRVTDTLCKQFGLVND
                     LSHMTKYRVIADLIDSKVLVSIDTAKRKKLRLAKKVTDMVP"
     gene            complement(490..5019)
                     /locus_tag="VN377_05010"
     CDS             complement(490..5019)
                     /locus_tag="VN377_05010"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27778.1"
                     /translation="MSWLKTCYDIQKDPVSFCSRGDQESAPSGGNQPPVFGTPIPGDG
                     SINNPLSFTWSIPINDPEGDQFSWTIQCNNGQSNSATGATNGTKSLSLSGLAYSTTYK
                     VWVNATDLTGSGLYTRRWLTFTTQGSGNSPPVFGTPSPVNGSTWNLLGLSWGIPISDP
                     DGNLFSWSIQCSNGQVSSGSGASNGTKSLTLSGLAYFTTYKIWVNATDPAGSGLYTRK
                     WYIFTTQAATSSWWNPGWNYRKLLTMDTTKISGTLVNFPILVQMTDSDLASHAQSTGN
                     DIVFVLYSDNTTKLNHELEYYNGATGVLYAWVNITRLSSSVNTKIWMYYGNPGCSSQQ
                     NKYGTWNSKYKAVYHLDENGVPFVDSTNNKNNITSANVNGTGMAQTGGIYKWLHFNGT
                     TNYLVVPFTLTGAYTVEAIANPDQITASGVVYSNEKTNSPVTGIAMGISSTAVYVTDG
                     TLHRGLSPSSTYTPAKTTKWWADVVFAVGSKIWFYLDGVNRTLTSQSYFTNYANYHTI
                     GARYNGKYSGFWDGFIDEVRISSGSWNVSWLKTCYDIQKDPTSFCSDGSEEQAPSGSW
                     TNRPPTFSNPSPVNGSSGVIRYHATNVTVSDLDGNLTTVCFWYGSSISGPWTKAQQNN
                     SVVANTTVRDRNSSYSSGWNTQYWWKVTASDGHVNSSVIFTFMTQSQSWVNRAPTFSN
                     PSPVNDSTGNWLGVPPAGSWIPWNIPINDPEGNRFSWTIQCSNGQTTSGTSPFNGTEY
                     IALSTLSYSTTYKVWVNATDPTGSGLWTRRWYTFTTRRSGNNPPMFTRVPSPENGSTA
                     TGSWLGPSWMNMTWSIRINDLENNSFNWWIQCSNGQGNSSTGAKNGTKSLRLSYLAYS
                     TTYKIWVNATDPTGSGLYIRRWFTFTTKANDPPILGTTSHVNGSIGNSLSFGWWGIFI
                     YDLEGNPFSWTIQCSNGQGNNGVGASNGTKSFALSGLAYSTSYTVWVNATDLTGSGLY
                     TRRWYTFTTQGSGGNNPPVLGVPSPVNSSSGSRLSLSWSIQINDPEGTVFSWTIQCSN
                     GQVTSGVGASNGTKSLALSGLAYSTSYKIWVNATDPTGSTLYTRKWYTFTTEPIIPIS
                     IPTAKAWSPVTSAAGETRVGAAVADINNDGRMEIIRSGINGIVVHNGTTGAVIWNKLS
                     TMWDSHIPLEIIDLNNDGYLDVISSYDTGTRALSGKDGSQLWYNANAPLYNKHAVTGD
                     INGDGYPEVFVCTAGANSGSPQGWVTALTHDGQIFAKVHTYFPCYGGLSLGDTNNDGV
                     YELFIGERNIGYDGNVVGKGVRAFWASNLTERWCHPEMLSSSNCPTLVDTNKDGILEV
                     VSLAQTGGNGIAVYNSADGSVIHYSNIPGLRCHSNPTVYDIDGDGNLEIIVGGGSDAW
                     GKPIVWDLYTWSLEAWLPFNCWEPPAIADINGDGRVEILECTITNISIFDDNYVFRGS
                     IPLNNNRSGSGYYGMSMIVAQDIDKDNLLELVINRYNQVYVYDTNGAAPTPRALSQFS
                     YYSQHRGRSPYFTQY"
BASE COUNT         1452 a         1227 c          955 g         1410 t
ORIGIN      
        1 cttccatcct atataagata gcattttatt cttctctatt aaaatttctt gtttcatagt
       61 agtaaaaaga tatcttacgg aaccatatcg gtgacttttt tcgcgagacg taactttttc
      121 cgtttcgctg tgtcgatcga aaccaacacc ttcgaatcaa tcaaatcagc gatcacacgg
      181 tatttggtca tatgactcag atcgttgacc aacccgaact gtttgcagag agtatcggtc
      241 acccgaatcg cactttcaat gcttttacaa atctcattct gatgtgtcag aaacacaaga
      301 gtcaccaggc ttgcatacgt cgaaggtttc ataggctcgt tcctccaaat ttttaatctt
      361 caatccaaga gagtaaacag attcacctat taaaataatt atatcctaga tagtaaaaga
      421 aaaaagaagg aattgttaag gttatccctt acttcgatgt ttttatggat taacactgat
      481 ttacttcttt taatattggg taaagtacgg tgatcgaccg cggtgttgac tataatagct
      541 gaattgagat aaagctcgtg gggttggtgc ggctccattg gtatcataga catagacttg
      601 gttatatctg ttaataacta attctagtaa attgtcttta tcgatatctt gtgctacaat
      661 cattgacata ccgtaataac cggaaccact tctattatta ttgagcggta tggatcctct
      721 gaacacgtaa ttatcatcga agattgagat atttgtaatg gtgcattcaa gaatctcaac
      781 gcgtccatcc ccattgatgt ccgcgatagc cggaggttcc caacagttga atggaagcca
      841 tgcttcaaga gaccaggtat aaagatccca gacaatcggt ttcccccaag catcactgcc
      901 accacctact atgatctcca gatttccatc accatcgata tcatacactg ttggattcga
      961 atgacatctc aaaccgggga tatttgaata atggataacg ctaccatcgg cactgttgta
     1021 gacagcaata ccgttaccac ctgtttgagc gagggagaca acctcgagta ttccatcttt
     1081 gttcgtgtcc acgagtgttg gacaattgct actagataac atctccggat gacaccatcg
     1141 ttccgtgaga ttcgatgccc agaaggctct caccccttta cctaccacat taccatcgta
     1201 accaatattt ctctcaccta taaagagttc atacacaccg tcattattgg tatctccaag
     1261 tgataatcca ccataacatg ggaagtatgt atgtactttt gcaaaaattt gtccatcatg
     1321 cgtaagagcg gttacccatc cttggggaga accactattc gccccagccg tacaaacaaa
     1381 tacttcgggg tatccatctc cattgatatc accagtaaca gcgtgtttat tatataaggg
     1441 tgcatttgca ttgtaccata attgagagcc atcttttcca gacagagctc tggttccggt
     1501 gtcatatgaa cttatcacat caaggtaacc atcgttgttg agatcaatga tttctagtgg
     1561 gatatgacta tcccacatcg tggataattt attccaaata acagcacctg ttgttccgtt
     1621 gtgtacgaca ataccgttta tccctgatcg tatgatttcc attcgcccat cgttattgat
     1681 atcagcaaca gcagccccca ctctagtttc tccagctgcc gaggtcacag gagaccatgc
     1741 ttttgctgtc ggaatactaa taggaatgat aggttctgtg gtgaacgtgt accattttct
     1801 tgtatacaga gtactcccag taggatcagt tgcattcacc cagattttat agctggttga
     1861 gtacgcgagt ccagaaagag cgagagactt tgttccgttc gatgcaccga ccccactagt
     1921 aacttgtcca ttgctacatt gaatagtcca agagaagaca gtcccttcag gatcatttat
     1981 ctggatgctc caggatagac tcaaccggct ccccgaagaa ctatttactg gagaaggcac
     2041 gcctaacacc ggtggattat taccaccact tccctgggtt gtaaatgtat accatctgcg
     2101 agtatacaac ccactaccag ttagatcagt tgcattgacc cagactgtat agctggttga
     2161 gtacgcgagt ccagaaagag cgaaagactt tgttccgttc gatgcaccaa ccccattatt
     2221 accttggcca ttgctacatt gtatagtcca agagaaggga ttcccctcaa gatcataaat
     2281 gaaaataccc caccaaccga aacttaacga gttacctata gaaccattca catgagaagt
     2341 cgtacctaat attggcggat catttgcctt tgtagtgaac gtaaaccatc tgcgaatata
     2401 taatccacta ccagttggat cggtcgcatt cacccagatt ttgtaagtcg ttgaatacgc
     2461 tagataggac agtctaagtg attttgttcc gttctttgca ccagtgctac tatttccttg
     2521 tccattgctg cattggatcc accaattaaa gctattattc tcaagatcgt tgatccggat
     2581 gctccaggtc atattcatcc aggacggacc caaccagctc cctgtagccg tagaaccatt
     2641 ctctggagaa ggtactcgag taaacatcgg tggattatta ccacttctcc gtgtagtaaa
     2701 cgtgtaccat ctgcgagtcc atagaccgct cccagtagga tcagttgcat ttacccacac
     2761 cttataagtc gttgaataac taagtgtaga gagcgcaata tattcggtcc cattgaatgg
     2821 acttgttcca ctagtagttt gtccgttgct gcattggatg gtccatgaga agcgatttcc
     2881 ctcaggatcg ttgattggaa tattccaagg aatccaagat cctgcaggag gaacacccaa
     2941 ccagtttccc gtagaatcat tcaccggaga aggattacta aacgttggtg ctctgttcac
     3001 ccacgattga gactgcgtca taaacgtaaa aatcacagac gaattcacat gaccatcaga
     3061 tgctgtcacc ttccaccaat actgagtatt ccacccactt gaataacttg aattacgatc
     3121 cctcacagtc gtattcgcaa caacactatt attctgttga gcttttgtcc aaggaccaga
     3181 aatactacta ccgtaccaaa aacacacggt cgtcaaattc ccatccaaat cactcactgt
     3241 gacattcgtc gcatgataac gaatcacccc agaactccca ttaacaggag aaggattact
     3301 aaacgttggc ggcctattcg tccacgatcc tgatggtgct tgttcctcac taccgtcact
     3361 acaaaaactc gtaggatcct tctggatatc ataacaggtt ttcaaccaac tcacattcca
     3421 agaaccactc gatatccgaa cctcgtcaat aaaaccatcc cagaacccac tatattttcc
     3481 attatacctc gcaccgatag tatgatagtt agcataattc gtaaaatagg attgagaagt
     3541 taatgtccga ttcactccat ccaggtaaaa ccatatcttc gagccgacag cgaaaacaac
     3601 atcagcccac catttcgtag tcttcgccgg ggtatacgtc gaagaaggcg aaagtcctct
     3661 atgtaaagtc ccatcagtga cgtacacggc agtacttgaa atacccatgg ctataccagt
     3721 gaccggtgaa tttgtctttt cattcgagta tacaacacca gatgcggtaa tctgatctgg
     3781 attggctatt gcttctaccg tatatgcccc ggtcaaggtg aaaggaacaa ctaagtagtt
     3841 tgtagttcca ttgaaatgaa gccacttgta aatcccacca gtctgagcca tcccagtacc
     3901 attcacatta gccgacgtga tattgttctt attgtttgtg ctatcgacaa atggaacccc
     3961 attttcatca agatgataca ctgctttata tttcgagttc caagtcccat atttattctg
     4021 ctgactacta caaccagggt tcccataata catccaaatc ttcgtattga ctgagctact
     4081 caaacgagtg atattcaccc aggcatataa cacgccagta gcaccattgt aatattctaa
     4141 ttcatggttg agtttcgtcg tattatcact ataaagcacg aaaacgatat cattccctgt
     4201 cgattgtgca tgagacgcta aatcactatc cgtcatctgc accagaatcg gaaagttcac
     4261 caacgtccca gatatcttcg tcgtatccat cgtcagaagt ttacgataat tccaccctgg
     4321 attccaccag gaactcgtag ctgcttgtgt ggtgaagatg taccattttc ttgtatacaa
     4381 accactgccg gcaggatcag tcgcattcac ccaaatctta tatgttgtga aataagcgag
     4441 cccagagaga gtaagagact ttgttccatt agatgcccct gatccactac taacttgtcc
     4501 attgctacat tgaatagacc aagaaaagag attcccatca ggatcactga tcggaatgcc
     4561 ccaggacaga cccaacagat tccacgtaga accattcacc ggagatggcg ttccaaacac
     4621 cggcggacta ttaccacttc cttgcgtcgt aaacgtaagc cacctgcgag tatacaaccc
     4681 actaccagtt agatcagttg cattgaccca aaccttgtaa gtcgttgaat aggcaagacc
     4741 agaaagtgac agtgattttg ttccgttggt tgcaccggtt gcgctgttcg attgtccgtt
     4801 attacattga atggtccaag agaattgatc accctcagga tcattgatcg gaatgctcca
     4861 ggtaaaactg agaggattat ttatagaacc atcaccggga ataggtgtcc caaaaaccgg
     4921 tggttgattc cctccagatg gtgctgactc ctgatcacca cggctacaaa aactcacagg
     4981 atccttctgg atatcataac aggttttcaa ccaactcaca ttccaagaac cactcgatat
     5041 ccga
//
LOCUS       DATMXJ010000191         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_71307, whole genome shotgun sequence.
ACCESSION   DATMXJ010000191 DATMXJ010000000
VERSION     DATMXJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71307"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1080
                     /locus_tag="VN377_05015"
     CDS             19..1080
                     /locus_tag="VN377_05015"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HWR27779.1"
                     /translation="MMADDSKNIALIDKANMLDGLARFPEQIKESLAIAEAAERFNFL
                     KIDNVVIAGMGASAISGDIMASLFRDKLDVPLIVNREYDLPKWVNKDTLVICISYSGN
                     TDETLSSFKIAYQKKCKIICISTGGKLQDFAEKRQVPFVKIPSGIQPRAATAYLLFPS
                     IIFLKKIGLLKTMIDADIEETIAVTQEFVAMNNKSVPEENNQAKQIAKKIHNTIPQIY
                     GWGIYTPIAIRWRHQLNENSKVIARTDIAPECNHNDIVGWSQNPDISKQFSCIMLRDK
                     DEETLDMTTRLNFMRDLFHNTAGTVIDVSPKGKSQLAKMMYLMCLGDFTSCYLAVLRG
                     IDPSPVDIITELKKRLAEK"
     gene            1187..2371
                     /locus_tag="VN377_05020"
     CDS             1187..2371
                     /locus_tag="VN377_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27780.1"
                     /translation="MYKKTLMNFLSLILLMLIVNTIFSPTACSQNEPELLIEVYDSNN
                     WSAIPEDIVFEGKSYLITVSTENITAIPEVNITVLGTTYLTNISEPYITIEIPPFNES
                     DSFIITATKEGYRPGTIQLTVLKGELTIKTDKVLIDENTEFQVTVTDQDNNPVEDALV
                     YVTEDASPMYTNQQGKALVQAPEIDILTTTTIQVIKSGYLPGSTTIRIENVEGSIFNL
                     TESKFLQILPVLLAILVVIVSVLYVFLRQRRAPTKMPYNSQGKTPDEPRHYHKEKQQR
                     FKTETDTYPKTEKRNISRSNSESRVEEIRIPVQAKKRGTTILTEEKEAEQIPEDENKH
                     PDEWFKGQDYMRYKIDELTGKIDQNTDGKWFEGEQDTKYKVDEALKKNLKKKKVEEDS
                     NE"
     gene            2460..3470
                     /locus_tag="VN377_05025"
     CDS             2460..3470
                     /locus_tag="VN377_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HWR27781.1"
                     /translation="MKLMFELSKEHATLPSAEVIATLNAEHINASLISSNEDVLIVET
                     NPNDNDLKRLAERLAFTFVIDELLFSCPTTLNEIIQNAEKHQLPPEGSIAIRCKNRSS
                     HIRSEQLIDQLGDIYTKKRTVNLTKPDIEVRAVITEDTAYLGIKKADLDTSHFQQRRG
                     HLRPFLSPITLHPKIARAMVNLSQVKKHQTLLDPFCGTGGILLEAGFIGAQIVGGDIE
                     QKMVDGSQKNLEYYHLKNYRLYCTDIGDIARYAPSVDAIVTDFPYARATTTKGEQLKQ
                     LYKRAFETISQILKKNRYAVVGLSNEDIRTIGEQYLSIVATYPVKSHRSLTRYFVVYK
                     HG"
BASE COUNT         1224 a          851 c          721 g          902 t
ORIGIN      
        1 gagatgagat ggtaaaaaat gatggcagat gatagtaaga atattgcact aatcgataaa
       61 gcaaatatgc ttgatggact cgcccgcttt cctgaacaaa tcaaagaatc gctagcgatc
      121 gctgaagcag cagagcgatt caactttctc aaaatagata atgtggtgat cgctggaatg
      181 ggagcctctg cgatttctgg tgacatcatg gcaagcctgt tccgggataa actggatgtt
      241 cctttgattg tcaaccgaga atacgatctc ccgaaatggg tcaacaaaga cacccttgtc
      301 atctgtatca gctactcagg aaacacggat gagaccctta gctcatttaa aatagcgtat
      361 cagaaaaaat gcaagatcat ctgtatctcc accggaggca agcttcagga ttttgcagaa
      421 aaacgacagg ttccctttgt gaagatcccc tctgggatcc agccgcgtgc agcgactgcg
      481 tatctcttat tcccctcgat tattttcttg aaaaaaattg ggttactgaa aaccatgatt
      541 gacgcagaca tcgaggaaac cattgcggta acccaggagt ttgtcgcgat gaacaacaag
      601 tcagttcctg aggaaaacaa ccaggcaaag caaatagcaa aaaaaatcca taacaccatc
      661 ccgcagatct acggatgggg catctataca ccgattgcga ttcgatggcg tcaccagctc
      721 aacgagaaca gcaaggtgat cgcacgcaca gatatcgctc cagaatgtaa ccataatgat
      781 atcgttggtt ggtcgcagaa ccctgacatc tcaaagcaat tctcctgcat catgcttcga
      841 gataaggatg aagagaccct cgatatgaca acccggttga atttcatgcg tgacttgttt
      901 cacaacaccg cagggactgt cattgatgtt tcaccaaagg gaaaaagtca gcttgcgaaa
      961 atgatgtacc tcatgtgcct tggtgatttc accagctgct atcttgcggt gttacgagga
     1021 attgacccaa gcccggttga catcatcacc gagctgaaga aacgcctcgc agaaaaataa
     1081 acctcgcatg ttttttcctt accttttttc aatttcttca aaaaacataa atacgtatta
     1141 ccccatcttc gcctacagca ctcaaaggca gttgatgagg attcgtgtgt acaaaaaaac
     1201 tctcatgaat tttctgagtc ttattcttct gatgttgata gtgaatacga ttttttctcc
     1261 tacagcttgt tcacaaaatg aaccagagct cctgatcgaa gtctatgatt caaacaactg
     1321 gagtgcaatc ccggaagaca tcgtctttga aggaaaaagt taccttatca ccgtcagcac
     1381 ggaaaatata acagcgattc ccgaggtgaa tatcaccgtt cttggaacca cttatctgac
     1441 gaacatctct gagccctaca ttactattga aatcccacca ttcaatgaat cagactcgtt
     1501 tatcatcacc gcaacaaaag aaggctatcg accaggtact atccaactaa ccgtacttaa
     1561 aggagagttg accattaaga cagataaggt tcttatcgat gaaaatactg agtttcaagt
     1621 caccgtgaca gatcaagaca acaatcccgt ggaggacgcc cttgtctacg tcacagaaga
     1681 tgcgtcacca atgtatacca atcaacaagg aaaagcgtta gtccaagccc ctgaaataga
     1741 tattcttacc accacgacga tccaggttat caagagcggg tatctccctg gttcgacaac
     1801 gattcgaata gaaaatgttg aaggatctat cttcaacctg accgaatcga aatttcttca
     1861 gattctccca gttctccttg ccattctcgt cgtcatagtc tcagtactgt atgttttcct
     1921 aagacaaaga agagctccaa caaaaatgcc atataactca caaggaaaaa caccggatga
     1981 accacgacac tatcataaag aaaaacaaca gcgattcaaa accgaaacag atacatatcc
     2041 taaaactgag aaacgaaata tctcaagatc aaactctgaa tcaagagtcg aagagatacg
     2101 tatccctgta caggcgaaga aacgggggac caccatcttg acagaagaaa aagaagctga
     2161 acagatcccc gaagatgaga acaaacaccc tgatgaatgg ttcaaaggac aggattatat
     2221 gcgatacaag atcgatgaac tcacaggtaa aatcgatcaa aacaccgatg ggaaatggtt
     2281 cgaaggggaa caagacacca aatataaggt cgatgaggca ttaaagaaaa acttaaagaa
     2341 gaaaaaagta gaagaagatt ctaacgaata agttttcacc aaatctgttc gttcatccat
     2401 tcgacccatt attttattag gagtaccgta ttcagagctt ctggattgtt tgattcccta
     2461 tgaaactgat gtttgaactt tcgaaagaac atgccacgtt accctctgca gaagtgatcg
     2521 caaccctaaa tgctgaacat ataaacgctt cactcatcag ctccaatgaa gatgtcctca
     2581 tcgtggaaac aaacccaaat gataacgacc tcaaacggtt ggcggaacgt cttgctttta
     2641 cattcgtgat cgatgagctt ctgttctcct gtccaaccac attgaacgag ataatacaaa
     2701 acgctgaaaa acatcagctt cccccagagg gatcaattgc gatccgatgt aaaaatcgct
     2761 catcacacat ccgttcagaa caactcatcg accaattagg agacatctat acgaagaaga
     2821 ggacggtgaa tctcaccaaa cctgacatcg aagtacgagc agtaataaca gaggacaccg
     2881 cgtaccttgg gataaaaaaa gcagatcttg acacaagcca ttttcaacaa cgacgaggtc
     2941 atttacgacc gttcctttcc cctataaccc ttcatccgaa aatcgcgcgg gcgatggtca
     3001 atctctccca agtgaaaaaa catcaaacgt tgcttgatcc tttctgcggt actgggggta
     3061 ttctgcttga agcaggattt attggagcac aaatcgttgg gggcgatatc gagcaaaaaa
     3121 tggtagatgg cagtcaaaaa aacctcgaat attaccacct gaaaaattat cgtttatact
     3181 gcaccgatat tggcgatatc gcacgatatg ctccctcggt tgatgcgata gtgactgatt
     3241 ttccctatgc tcgcgcaacc accacaaaag gagagcaact caagcaattg tacaaacgtg
     3301 cattcgaaac catatcacaa attcttaaga aaaacagata tgctgtcgtc ggactctcaa
     3361 atgaagacat acgaactatt ggggaacagt atctctctat cgtcgcaaca tatccagtga
     3421 agtcccatcg gagtctcacg cggtatttcg tggtgtataa acatgggtaa aaaatgtatc
     3481 aaaaaatccg aaagttatcc tttaaaaaac gacattagac cgaatatcaa tacaacaata
     3541 tgtgtcacct attgtcgaaa aaacatcagc cacactctat ttgtgttata ttaagatgta
     3601 attatcacac gactaagaaa atactcttcc cccttttttt tcttctttga tcggatagct
     3661 ttaaccaaaa cgcttttacc cccgcaggga gtatccgt
//
LOCUS       DATMXJ010000192         6429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_14461, whole genome shotgun sequence.
ACCESSION   DATMXJ010000192 DATMXJ010000000
VERSION     DATMXJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14461"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..735
                     /locus_tag="VN377_05030"
     CDS             277..735
                     /locus_tag="VN377_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HWR27782.1"
                     /translation="MGKMKANRKFIEADERAVSAVIGVILMVAITVAIAATVYVYVSG
                     MIGGTKTQTPNVACTTDATANKITVATADAAIKWSDIVITNDSGVTLNWDIYTGGGVT
                     ATGVHVVPASTILVTAGDYIYLSGTFIGNSKITLMYKPTNSLLGSWTVNV"
     gene            857..1258
                     /locus_tag="VN377_05035"
     CDS             857..1258
                     /locus_tag="VN377_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HWR27783.1"
                     /translation="MFREFNQRKQAVSAVIGVIIMVAITIAMAAVAYAYFTGMIGEQK
                     IDAPVIDFVKSDSEKTITISTADIDANWNDINITFTNATSYDYLTKTGAVTAGDKIDL
                     TIDQSLRGKVTITFIHKPSNSLLGTYTIDNV"
     gene            1393..1851
                     /locus_tag="VN377_05040"
     CDS             1393..1851
                     /locus_tag="VN377_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HWR27784.1"
                     /translation="MKSNKKFIEMKDNGVSAIIGVILMVAITVAIAATVYVYVSGMLG
                     GIKDHTASVACIADSSTNRITITSASANLKWRDIVVTTDNTSVNWRVYNPNDNPLDAA
                     KSTSGATADIEAGDYIEFDFTANPWMSGNIRASLRFVPTNTLLGSWIITV"
     gene            complement(1886..2878)
                     /locus_tag="VN377_05045"
     CDS             complement(1886..2878)
                     /locus_tag="VN377_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWR27785.1"
                     /translation="MFEDLKGPGLLIHHWDTDGICSARLLLEKLPKDIVNKTPVLGNY
                     FLTEEELTAYSSYDYVIVVDMALPEENILRLAKHAKVLIFDHHLQQQIPGVFHQNPIA
                     KGMDPLLYPSASWIVNEYIGNSVNLFALLGIVGDFEKRIQTNKTIYTLISDFCSKQQL
                     TFDELLTMVYLLDSNYKIGDRKAVEDAPHLLLKYTDTQRILQNIRWKNNLSLLEKEIA
                     KYIDVPCEEKEHVLLKRIHTKFNIISTVTRKIAWNAGKDTVVVNTGFFTDRDQIYVRS
                     GKDLQPLIQQGKMLGYRCGGKKEVLGAVVPKEKTESFVKEILRYLSISTESKNP"
     gene            2949..3665
                     /locus_tag="VN377_05050"
     CDS             2949..3665
                     /locus_tag="VN377_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HWR27786.1"
                     /translation="MMVDVTILNAIRLITGTVLLAYASYTDVKTRRAANILWVIMAII
                     GAILLLIQYLDGGFPNIWYLVFIPIMIALMYLFFQMRLLFGGADAKALMALAILVPIQ
                     PIMGDLPIWLSFMPGSWIIFANATILFLLIPISLLIYNIGKHNIQFPHSLLGYVISVE
                     KAKQKFVWPLEKIKDGKRKLMYMPKNFDIDQELAEFEKQGITEIWVTPKIPFMIPLLI
                     GFLLSFLLGDLLLQLVRVLF"
     gene            3734..4840
                     /gene="dnaJ"
                     /locus_tag="VN377_05055"
     CDS             3734..4840
                     /gene="dnaJ"
                     /locus_tag="VN377_05055"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWR27787.1"
                     /translation="MEKKDYYDILGVDHSATKDDIKKAYRKLALKYHPDKNKAKDAEE
                     KFKEISEAYAVLYDDEKRKMYDQHGHAGIDQQYTTEDIFRTTDFGDIFRGTGFDFNDI
                     FEQFFGRRGGYTNRQQAQRGNDIRYDIQISLEDAYRGMNTELEVPRTELCDTCKGSGA
                     RPGSSPKRCSTCGGTGQMQVSRRTAFGMFTQISECSKCHGQGTIIEDPCPVCRGRGSI
                     QKTRKIEVHIPKGIDDGAHLRLAGQGEHPKGVNQSGDLYVVVHMKGHPVFERRGADLY
                     RKLNVSFPQASLGTTLTVETLQGGEKLKIPEGTESGTLFKLKGSGMPKIQGLGYGDLY
                     VLIQVTTPKKLSKRARLLLEEVERELEYSGETYK"
     gene            4893..5486
                     /gene="hsp20"
                     /locus_tag="VN377_05060"
     CDS             4893..5486
                     /gene="hsp20"
                     /locus_tag="VN377_05060"
                     /inference="COORDINATES: protein motif:HMM:NF041800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HWR27788.1"
                     /translation="MDPFDDENHEKKKNPHDPFRGDDDIFRDIFNDNRIKDIFNDDKV
                     MDDIKKMAEEMMKMFTNAQPGKPIVHGYKIEFGPDGKPQIEDFGNRSVRSPEGVRTIS
                     DEIEPLTDIIEGENEVAITVEIPGVEKEDINLVATEDTLEIKVDSPKRKYHKRIDLPC
                     NVKTKSTKATYKNGILDIVLDKKEKKKDQSGFKVSIK"
     gene            5575..>6429
                     /locus_tag="VN377_05065"
     CDS             5575..>6429
                     /locus_tag="VN377_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR27789.1"
                     /translation="MVNVTFYGGVNEIGGNKILLDDHGTKIFLDFGMSFKRRGKFFEE
                     FLNPRTANGIGDFLEMGLIPDIPGIYRSDLLEHHGRKSEEPSVSGVVLSHAHADHANY
                     ISFLHKDIPIYCGETCKRIINAVAEQTTRDLENEVIDFRIRPLYRSGYKIPPVIRKFY
                     TFTTGKKFKIDSIEVEPYHVDHSVPGAYAFLIHTSEGPLIYTGDLRMHGLHADMTDDF
                     IEAARQAKPIAMITEGTRIDKKKTNESEQKIYHDSKKKIQQCDTLSMVDFNFKDVDRF
                     TTFYKIAKD"
BASE COUNT         2130 a         1345 c         1274 g         1680 t
ORIGIN      
        1 cgtcagaatc agtgatgttt tatcgttttg taatttgact gtttctgcat ttttcctacg
       61 agagtataaa tagagagaac atgaaaatag atggtagaaa ttggtatgtg taagcatact
      121 tggcatagag agaaataaaa ataaaaaccg attgaaagac aacagatgaa ggataaggag
      181 gcacttgatg attcatctgt gtttcaaaaa gtttttatac caagtatcat atgcatcttc
      241 caataaaaac agctttgaaa aaaaagctag gagagaatgg gtaaaatgaa agcaaataga
      301 aaatttatag aagcagatga acgcgcagtc tccgctgtta ttggtgtcat tttaatggtt
      361 gccataacag tcgcaattgc tgccacagta tacgtctacg tgagtggaat gatcggtgga
      421 acgaaaacac aaacccctaa tgtagcctgt acaacagatg ctactgcaaa taagataacg
      481 gtcgctactg ctgatgcagc tataaaatgg tcagacatag taattacgaa tgattccggt
      541 gttacattaa attgggatat ttatactggt ggtggagtaa ccgcaactgg tgttcatgta
      601 gtgcctgcga gtaccattct tgtaactgct ggtgattata tatacctcag tgggacattt
      661 ataggcaaca gtaaaattac cctaatgtat aaacccacaa actcattact tggctcgtgg
      721 acagtcaacg tctaaaccac aaccaatctt ttcttctttt attttttata tttttttctt
      781 tcaacaaaaa ccctacagat attaagaaac ttcattaaaa aaaaatggaa tggaggagac
      841 aagatacatt atgataatgt tcagagaatt taatcaacgc aaacaggctg tttcagcggt
      901 catcggtgtc atcatcatgg ttgcaattac catagcaatg gctgcggtcg cttatgcata
      961 ttttacaggc atgattggcg aacaaaaaat agatgcacca gtcattgatt tcgtaaaatc
     1021 agattcggaa aaaacaataa caatatcgac agctgatata gatgctaatt ggaatgacat
     1081 taatataacg tttacgaatg caactagtta tgattatctc acaaaaacag gtgcagttac
     1141 agcaggagat aagatagatt taactatcga tcaaagtcta agaggaaaag taaccattac
     1201 atttatacat aaaccatcca attcactgct tgggacatat actattgata atgtttaaac
     1261 atttagttat atctattaaa aatcgaaata aacatatttt gattaaagac aatttaaatt
     1321 ttttttaaaa aggttttata ttatcttcac cgttaagcta ttaacaaaaa aagaaaaatg
     1381 agaacgaaca acatgaagtc aaataaaaaa ttcattgaaa tgaaagataa tggtgtttcc
     1441 gcaatcatcg gtgtaatcct aatggttgcg ataacggtcg caattgctgc aacagtatat
     1501 gtttacgttt ctggaatgct gggcggaata aaggatcata cggcgagcgt cgcatgcatc
     1561 gctgattctt ctactaatag aattactatt acttctgcat ctgcaaattt aaaatggaga
     1621 gacatagtag tgactacaga taatacatca gtaaattgga gagtgtataa tcctaatgat
     1681 aaccctttag atgcagctaa atctaccagt ggagcgacag ctgatataga agctggtgac
     1741 tatattgaat ttgattttac cgccaatccg tggatgtcag gtaatattcg agcatctttg
     1801 agatttgttc cgaccaacac cttacttggg tcatggatca taacggttta aacaaatcac
     1861 acaaaaaatc aaaatgcgcc attttttatg ggttttttga ttcagtagaa atggagaggt
     1921 aacgcaaaat ctctttcaca aacgactcgg ttttttcctt tggaaccact gcgcctagaa
     1981 cctctttttt cccaccgcag cggtatccaa gcatctttcc ttgttgtatc aacggttgca
     2041 aatccttacc acttcggaca tagatttgat ccctgtctgt aaaaaatcca gtgtttacca
     2101 ccacggtgtc cttcccagca ttccatgcta ttttccgcgt caccgttgaa atgatattaa
     2161 acttcgtatg aatccgtttt aaaagaacat gctctttttc ttcacaagga acatcaatgt
     2221 acttcgctat ctctttttca agtaaggaga gattattctt ccatcgtata ttttgaagaa
     2281 tcctttgtgt atctgtatac tttaaaagaa gatgcggtgc atcttcaacc gcttttcgat
     2341 caccaatctt ataattggaa tcaagtaggt acaccatcgt taacaactca tcaaaggtca
     2401 actgctgctt tgaacaaaaa tcagaaatca atgtatagat tgttttgttt gtttgaatgc
     2461 gtttctcaaa atcaccaaca atgccaagca aagcaaacag attcaccgaa ttcccaatgt
     2521 actcgttaac gatccagctc gcagatggat acaacagcgg atccattcct tttgcgatag
     2581 gattctgatg aaacacacca ggaatttgtt gctgcagatg atgatcaaaa atcaaaacct
     2641 tcgcatgttt tgctaatcgc aggatatttt cctcaggtaa cgccatatca acaacaatca
     2701 cataatcata cgatgaatac gcggtgagtt cctcttcagt gagaaaataa ttcccaagca
     2761 ccggtgtctt attgactata tcttttggga gtttttcaag caggagccgc gctgagcaga
     2821 tcccatcggt atcccaatga tggatcaaca acccaggtcc tttcaaatcc tcaaacatat
     2881 gtcaaacctt tttgcaaacg aaggagaaag caattacctt aatatggtct tttctgttac
     2941 caatttctat gatggttgat gtcacaatct tgaacgccat tcgattgata acagggacag
     3001 ttcttctcgc gtacgcctcc tataccgatg tcaaaacacg acgagcagca aacatcctct
     3061 gggtcatcat ggcaatcata ggagcaatcc tcctgctcat ccaatacctg gatggcggct
     3121 tccccaacat ctggtatctc gtcttcatcc caatcatgat cgcgctcatg tatctcttct
     3181 tccagatgcg cttattgttc ggcggagccg atgccaaggc acttatggca ctagcgatcc
     3241 ttgtccccat ccaaccaatc atgggagacc ttccgatttg gttatcgttc atgccaggat
     3301 cctggatcat ctttgcaaac gcaaccatcc tgttcctcct cattccaatc agccttctca
     3361 tctacaatat cgggaaacac aacatccagt tcccccacag tctccttggc tacgtcatct
     3421 cagttgagaa agcaaaacaa aaattcgtct ggccattaga aaaaataaag gacgggaaac
     3481 gaaaactcat gtacatgcca aagaacttcg atatcgacca agagcttgca gaattcgaaa
     3541 aacaggggat cacagaaatc tgggtgactc caaagatacc attcatgatc cctctgctta
     3601 tcgggttcct tctctcattt ctccttggag accttctcct tcaactcgtc cgagtactct
     3661 tctagaccaa aatgacgtga caggctcata ataaaaaaga aatacccctt ctaaatgtat
     3721 agtcgctagt cctatggaaa agaaagatta ttacgatatc cttggtgtgg accattctgc
     3781 cacaaaagac gatatcaaga aagcctatcg gaaacttgcg ttgaaatacc atcctgacaa
     3841 aaacaaagca aaagacgcag aagaaaaatt caaggaaatc tcagaagcct atgcagtcct
     3901 ctatgatgac gaaaaacgta agatgtacga ccaacatgga catgcaggaa tcgaccagca
     3961 atacaccaca gaggatatct tcaggaccac cgattttggt gatatcttcc gaggtacggg
     4021 atttgatttt aacgacattt tcgaacagtt ctttggaaga agaggtggat ataccaaccg
     4081 tcaacaagcc cagcgaggaa acgacatccg ctatgatatc cagataagcc tcgaggatgc
     4141 atatagagga atgaacactg aactggaagt cccacggact gaactgtgtg acacctgcaa
     4201 aggatccggg gcacgaccag gcagtagccc aaaacgatgc tcaacctgcg gaggaaccgg
     4261 acagatgcag gtctcccgtc gtaccgcttt tgggatgttc acccagatca gcgagtgctc
     4321 caaatgccat ggacaaggga caatcatcga agacccctgt ccagtctgcc gaggacgagg
     4381 aagcatacaa aaaaccagga aaatcgaggt acatatccca aaaggcatcg atgatggggc
     4441 gcatcttcga ctcgcaggtc aaggagaaca cccaaaggga gtaaaccaga gtggtgacct
     4501 gtacgtcgta gtccatatga agggacatcc tgtctttgaa cgacgaggtg cagacctcta
     4561 ccgcaaactc aacgtctcat ttcctcaggc aagtctcggg acaaccctga ctgtagagac
     4621 tttacagggt ggagaaaagc taaaaatccc tgaaggaaca gaaagtggga ctctctttaa
     4681 gctcaaaggc agtggaatgc ccaaaataca ggggttaggg tatggagacc tctacgtcct
     4741 catccaagta accacaccaa agaaactaag caaaagagca agacttctcc tggaggaagt
     4801 cgaaagagaa ctcgaatatt caggcgaaac atataaatag gagttttact aagtaatata
     4861 tcgaaaagaa gtaaaaaagg gggtagtgac ctatggatcc ttttgatgat gaaaatcacg
     4921 aaaaaaagaa aaacccacat gatccgttcc gaggagacga tgatatcttc cgggatatct
     4981 ttaatgataa caggataaaa gatatcttca atgatgacaa agtcatggat gatatcaaga
     5041 aaatggcaga agaaatgatg aaaatgttta cgaatgccca accaggcaaa cccattgtac
     5101 atggctataa aatcgaattt gggcctgatg ggaaaccaca gatcgaggat ttcgggaacc
     5161 ggtcagtacg atcccctgag ggagtccgca cgatctccga tgaaatcgaa ccgctcaccg
     5221 atatcatcga aggggaaaac gaagtggcca ttaccgttga aataccaggg gtcgaaaaag
     5281 aggatatcaa cctcgtcgcg accgaagata ccttggaaat caaggtggac tcaccgaaac
     5341 gcaaatatca caaacgtatc gaccttcctt gcaatgtgaa aacaaaatca acaaaagcga
     5401 cctacaagaa tggaatcctt gacatcgttc ttgataaaaa agaaaagaaa aaggatcaaa
     5461 gtggatttaa agtcagcatc aaataggcac tttccttttt tcttcttttc atttctttgt
     5521 acaaatgttt atgtatacta ctctatattt gtgagattgt aaacgggagc agaaatggtg
     5581 aatgtaacgt tctatggtgg tgttaatgaa atcgggggaa acaaaatcct gctcgatgac
     5641 catggaacga aaatctttct tgatttcggg atgagtttta aacgaagagg caaatttttt
     5701 gaagaattcc tcaaccctcg gacagccaat ggtattggag attttctaga aatggggtta
     5761 atccctgaca tccctggcat ctatcgatcc gatctccttg aacatcatgg gagaaaatca
     5821 gaagaaccaa gcgtttctgg tgttgtgcta tcccacgcgc atgcggacca tgcgaactat
     5881 atctcatttt tacacaaaga catccccatc tactgcggag aaacctgcaa aagaatcatc
     5941 aacgcagtag cagagcaaac aacgagagat cttgaaaacg aagtcattga tttccgaatt
     6001 agaccgcttt atcgttctgg atataagata ccaccagtga tacgaaaatt ctatacgttt
     6061 accactggaa aaaaattcaa gattgattcg attgaggtcg aaccatacca tgtggatcat
     6121 tccgtaccag gcgcctatgc gtttctgatc cacacgtccg aaggaccact catctacacg
     6181 ggtgatctca ggatgcatgg attacatgca gacatgaccg atgacttcat cgaagcagcg
     6241 cggcaagcca aacctattgc aatgattact gaaggaaccc ggatcgataa aaagaaaacc
     6301 aatgaatctg agcagaaaat ctaccatgat tccaaaaaga aaatacaaca atgcgatact
     6361 ctctcgatgg ttgatttcaa ttttaaagac gttgaccgat ttacaacgtt ctataagata
     6421 gccaaagac
//
LOCUS       DATMXJ010000193        13417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_8855, whole genome shotgun sequence.
ACCESSION   DATMXJ010000193 DATMXJ010000000
VERSION     DATMXJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8855"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1910)
                     /locus_tag="VN377_05070"
     CDS             complement(78..1910)
                     /locus_tag="VN377_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWR27790.1"
                     /translation="MIRKLVELFVCVLLMITPISCAIPTEHERSNPTQTLEHQNSIST
                     PVPQMCSYDGWELQWSHDYGGMGHSQLAQPVGDLDADGVNEIIVGGYENSGMCRILSY
                     NATQQTYVEEYSWYIPGGSYHIPTGACIFDLNEDGTLELVVSWGYTNADGVYAYTWDG
                     TTLTTLDCYNGVGVDFLYDVYACDYNDDGHVEVIVSNAPNMGTSQWHVYALRWEVDHF
                     VAEAFWACPNGASCECPMIWGGDVDNDQKTEIIADVSNDGSFTAGTWALSWNDTTEDW
                     DAVPVWTNYGSSTVYGDGIADIDEDGTPEIGVGSYGGTPSGWLFEWNGTGYEMVWHAE
                     YPGGEPVIESVALGDADNDGHQEFVFGTQQVHIIGWNGEAYYEEATLTDPQSMLAGMN
                     ICDFDTDGFNELKACEIISGSGTEFIWQYHVPDVIPPLTTCSLTGKMENGIFTSNVTI
                     TLIAVDNGSGIQYTKYKLDADEWMTYTEPFTVTDDGNHTLRFYSADRSGNIEEEQTRT
                     FTIQHHPILALTIKGGKGVSVMIENKGLLPVNKVAWTINLSGGIILKGISKEGLIPQL
                     LPGQQKILTSSIIGFGKTIITATVDDTHTTAKAFVLLIFVIGVQ"
     gene            complement(2008..2805)
                     /locus_tag="VN377_05075"
     CDS             complement(2008..2805)
                     /locus_tag="VN377_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27791.1"
                     /translation="MKGGLTETQKKLIQWLEQNNHWFVTTKEIASFLACTEKEAHSLG
                     FALSLKKWFATLGRNQYLLLKTTQDRHLPSIHPLIIGSRLIDPYYFSFHTAASYHKLI
                     PPINQPVYLVTTTIRNNTDIRDTSYHFIHVTPRKFFGFNPVTIDDTPVNMADKEKTII
                     DSLEKFKYIGGLSEVIRILKNNIETLDVQRLVDYTVLMGSSILIQRLGYTLDHLNVSF
                     DEEFLQSYSLGVLTYFDPFNTYDMKPKRDEKWNLMINIPESLFNKEP"
     gene            3137..3712
                     /locus_tag="VN377_05080"
     CDS             3137..3712
                     /locus_tag="VN377_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWR27792.1"
                     /translation="MKVTCPHCKRRMRIFRNRKNVCACGKLLNYRHFLHEKIAYDVYL
                     VDANILIYAFDSRSTRRKYCQAILAMHASSMSIATTRQIIEEVGSAIEKQLPEMFLIY
                     NVSTIPGDLKEIKTNFLKQPSIADLSLVQAAYEHPEVRGIITYDNDFTRIATAGLVEQ
                     KASMKFWLGNAKEFVEKHRIRLPETEGEEGG"
     gene            complement(3781..4260)
                     /locus_tag="VN377_05085"
     CDS             complement(3781..4260)
                     /locus_tag="VN377_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27793.1"
                     /translation="MQRKKTVPIIVLAVLLITMIEFSGCTNQNNDGTNLTPARDVTGN
                     WTGSPVFTDRAYECAYEGTMKLRLEQQDSNVTGYFDLTVVSTDGSPACVQIGSTFRYL
                     VEGSISSSRIDLLVAGTDTLVGSFTTDLMTLRWEQCQECDSGPAVKLVGMVSLRKER"
     gene            complement(4735..5076)
                     /locus_tag="VN377_05090"
     CDS             complement(4735..5076)
                     /locus_tag="VN377_05090"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWR27794.1"
                     /translation="MDAKVDEYIKKQKSPQKEICQRLRSIILTTFPDINEQMKAGVPC
                     YGSTKKDPCGKYYIAALKNHVNLGCSLKGLTTRQQGLFEGGGKTMKHIKVYSVEEINE
                     EKIIRLLRIIQ"
     gene            5278..5571
                     /locus_tag="VN377_05095"
     CDS             5278..5571
                     /locus_tag="VN377_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27795.1"
                     /translation="MNQCFGAVLASMVLATYPVSLVGAQMTSAMKDQIHQDEIILLTV
                     KELADQGMDLNQLHSELQTMHGSMQSDNQKRGQGSLSLRGFCGNSLFSCRGFA"
     gene            5748..6707
                     /locus_tag="VN377_05100"
     CDS             5748..6707
                     /locus_tag="VN377_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27796.1"
                     /translation="MRTRELFLGCGIVLLVVCLCASSVGALSHSPDQPGPYHIGSYKV
                     WYLEPPYGIYRATIRYPATSDGQRAPVDSSAAPCSGIVVANGYAGSEWQITWIPEHLT
                     SYGYVTLCFTPPHRLSWDTTQWASGFLGGLRVLQNQNDRRFSPIYHVLDGETWGAIGL
                     SMGGGGCVEATGTTGSEIDASVALAPAGFPPVLAAARNITVPLQLQVGTVDRLVPPDR
                     VLDIYSNYLTNGTTKEYLAITGGNHIGFIDEKYASFGQRWNIDNPPGISVEQQHNISR
                     TYFTAWFQYHLRHLDEYYTYIFGEEAQQDLDDGILADLRYNIP"
     gene            complement(6745..8301)
                     /locus_tag="VN377_05105"
     CDS             complement(6745..8301)
                     /locus_tag="VN377_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HWR27797.1"
                     /translation="MKNSIFVILLSVMLLISPLVSVTKAKSTNGLEKEIIIESHPSTI
                     QQKNFMSTSSDWKMMMLNASDGEASDSFGSSVSVSGNYALIGAPFNDNITGAAYIFKH
                     IGTTWIQEAKLTAADSVPEDCFGWSVSLDGNTCVIGAIEDDDNGNRSGSAYIFTNSDT
                     AWMQQAKLLASDGAPIDSFGNSVSISENYAVIGACLDDNSTGSAYVFERKGTTWTQQA
                     KLLASDGKPDDHFGNSVSINGNHIVIGINMQGGENSTESAYVFERYGTTWTQEAKLVS
                     PDSEPTGFGTSVSIHGDHIMIGAPCFDFLTGAAYIFKHNGTTWTQQAQLRGSDGNPLE
                     LFGWAVSISENYATTIGMSFFEHNGSVYIFKRNGTTWSQDAKLTVFTNQFSGLSNMIS
                     STDTCVIVGNCNYNNSTGSAYVFWKPHPQFEITFKGSTTFVRNIGDANATNVFISRRL
                     DGGFFFMGKNKTVILPSIPVGETKEARLGMIIGFGKTTITLSISCDQGVNETSSKQAF
                     IFFFFLLGIK"
     gene            complement(8456..9046)
                     /locus_tag="VN377_05110"
     CDS             complement(8456..9046)
                     /locus_tag="VN377_05110"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27798.1"
                     /translation="MIKKRNYLLICMLVLVLVAPTAISLHTDILTPPNPPTINGPHCG
                     KINTNYTFTINASIDPEGDLYYFFLEWGDGNNSGWLGPYPSDQTFTVTHRWSEPGTYN
                     FRLKVKDPFGVESDWSEPFTIYITSKIILIGFVQSAGNQCENCTILNMSIALVIKLKP
                     IGLKLYSSIQVLLLNDEFKGLIGSHFFAVVGYGLVL"
     gene            complement(9273..10907)
                     /locus_tag="VN377_05115"
     CDS             complement(9273..10907)
                     /locus_tag="VN377_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27799.1"
                     /translation="MNLNKKIILRTGCIVILLLMMNLFSATGQQLHTRSDTFEPTLKN
                     QTSPMIIIVKPKPGSLYIQDKQVARLPLGRIQIIGKLTMVLEAADNSSEIMSIDIYVD
                     NIFKVRLQGPYYEWMLDDPLFFRHKIRIVTYDVYGTNVSTEFNINIWCLGRLPEEPST
                     PKVGTANQNNTDGYNYQRNKNGVGDFVYNQLWYFNFLDDRGTSDTSDDIAGVAAYGLA
                     NPENKLTGGGLTNTFGMIIRNSSEGTSFPLFSDNYDPTNPSNFSASPTFEPGPGPELQ
                     NPSGTIDVISPDHYHITGQVIKDSKEITWDLHYRRSLGQPWLPWVHWPVPKTLGIIPA
                     WITYHMQMANAVVNGIYTVKDGANQTTYNLVDAKGYHDGFYSEFVFSIFEWNWIDFKQ
                     DNLSVHLLYPHAPVYSCEEGWETCTPGNLRVVTNTTGEQKEYNFYRGCDLAKNEINVQ
                     YGNLATDPRYPDVKYPTQATITAEDDSGNKLIVQWSLLRYMIVYFDVPDPFYDTVTFE
                     IIANFTGTFYEADTGMTVPIAGTGWSDWSGEAFPQQ"
     gene            11183..11437
                     /locus_tag="VN377_05120"
                     /pseudo
     CDS             11183..11437
                     /locus_tag="VN377_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019606420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11636..12388
                     /locus_tag="VN377_05125"
     CDS             11636..12388
                     /locus_tag="VN377_05125"
                     /inference="COORDINATES: protein motif:HMM:NF016364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWR27800.1"
                     /translation="MDAETLQTPRETIEKKEGTCEDLSILLCSLLSNIGLTTYLVFTD
                     THVYAMVSDVNADDLWHVAEQSLIGHVEDIFGEPIMQSYYQTYELPSMGVLYAGGEEG
                     KTFAGVIDSMTIDYTIQSDQQLTLFVVPTWTEYSEFQSGDFANFTPSDQWNFTSTIGS
                     IPEMSTFGGIMLYNPSMQTATVTVDLLFSFQASFYKTYNQDALTVYSVWGKDAVLLDP
                     TLGDFGFPGYDVTVVGVKKAIDPVTKAYVTLS"
     gene            12516..12725
                     /locus_tag="VN377_05130"
     CDS             12516..12725
                     /locus_tag="VN377_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27801.1"
                     /translation="MVRIKEKDEADKQARERLENGTLKSLRSWYKILIEELEQTEEYY
                     NITIYQVPPYPPNTKEAKDFFIQRT"
     gene            complement(12870..>13417)
                     /locus_tag="VN377_05135"
     CDS             complement(12870..>13417)
                     /locus_tag="VN377_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWR27802.1"
                     /translation="IFLGSIFFLQETVIWENYIGILLILCGIYLVLAVRIFTVPRLDR
                     SFVYIAALIPLDVASALLIKWFLGNTEPIDLAVSIQLMAFLILGVLLLIMRRFTPPAP
                     SYVRPRARSVLVASLLAASAAVLLYTALSDANASKVYPLAGVSIITIFLLTTLFLKEK
                     SYPHRFIGTLIVFLGIYLISL"
BASE COUNT         3478 a         2740 c         3342 g         3857 t
ORIGIN      
        1 tttacataaa tatatagagg ttgagcggaa gaaacggttt tatctcagca agatcaaaga
       61 ataagaaaac aggggggtta ttggaccccg attacgaaaa tcagtaggac gaaggctttg
      121 gccgtggtat gtgtgtcgtc aacggtcgcg gtgataatcg ttttaccgaa cccgatgatg
      181 gaggaagtca agattttctg ttgacccggg aggagttgcg gaatcaatcc ttcttttgag
      241 atacctttca ggatgatgcc tccgctgaga ttgatggtcc aggcgacttt attcactggc
      301 aggaggcctt tgttttcgat cataacagaa acacctttac cacctttgat ggtgagggcg
      361 aggatcggat ggtgttggat agtaaacgtc cttgtttgtt cttcttcgat gtttccgctt
      421 ctatcagctg agtagaaccg cagcgtgtga ttcccatcgt ctgtcaccgt gaatggttct
      481 gtataggtca tccattcgtc agcatcgagt ttgtatttgg tatattgtat cccagatcca
      541 ttatcgaccg caatcagcgt gatagtcacg ttgctggtaa aaatcccgtt ttccatcttc
      601 cctgttaagg aacaggtcgt gagcggtggg atgacatcgg ggacatgata ttgccatatg
      661 aactcggtcc cgcttccgct gatgatctca caggctttga gttcgttgaa cccgtccgtg
      721 tcgaagtcgc agatgttcat acctgctaac attgactgcg ggtctgtcag ggtggcctct
      781 tcgtagtatg cctctccgtt ccaaccgatg atatggactt gttgtgtacc aaagacgaac
      841 tcttgatgtc cgtcattgtc tgcatccccg agcgcgaccg attcgatcac cggttctccc
      901 ccggggtact ctgcatgcca gaccatctcg tagccggttc cgttccattc gaaaagccag
      961 ccggacggtg tccctccata gcttccaacg ccgatctccg gtgtgccatc ctcgtcgata
     1021 tccgctatcc cgtctccgta tactgtggat gagccataat tcgtccaaac cggtactgca
     1081 tcccaatcct cggtagtatc attccaactc aacgcccagg ttcctgcggt gaacgaacca
     1141 tcatttgaga cgtctgcgat aatctcagtt ttttggtcgt tatcgacgtc accgccccag
     1201 atcatagggc attcacagct agctccattc ggacatgccc agaatgcctc agcgacgaaa
     1261 tggtcgacct cccagcggag tgcatagaca tgccattggc ttgtccccat gttcggtgcg
     1321 tttgagacga tcacctcgac gtgaccatca tcattgtagt cacaggcgta cacatcgtag
     1381 aggaaatcga cgccgacacc gttgtagcaa tcaagggttg taagcgtggt tccatcccag
     1441 gtgtaggcgt ataccccgtc tgcgttggtg tacccccagg agacaacaag ctcaagtgtt
     1501 ccgtcttcgt tgaggtcgaa gatgcatgcc ccagtcggga tgtgatagga acccccaggg
     1561 atgtaccagg aatattcttc tacataggtt tgttgagtcg cattatagga taggattctg
     1621 cacatccctg agttttcgta gcctcccacg atgatttcgt tgacgccatc tgcatcgagg
     1681 tctccgaccg gttgtgccag ttgtgaatga cccataccgc catagtcatg gctccattgc
     1741 agttcccagc catcgtaaga acacatctgt ggaacgggtg tggaaatcga gttctgatgt
     1801 tcaagggtct gggttggatt gcttcgttca tgttctgtgg gtattgcaca ggaaatcggt
     1861 gtgatcatca agagaacgca gacaaataat tcgacaagtt ttcttatcat tgattctact
     1921 cccttgatac ctatcgctga ttccttattt aatattttgt aaaagatata aacatttgaa
     1981 aaaaagggta agggattagc gttgtcgtta cggttctttg ttgaagagtg attctgggat
     2041 gttgatcatc aggttccatt tttcgtctcg cttcggtttc atatcatagg tgttgaaagg
     2101 atcgaagtag gtcaagacgc ccagggagta gctttggagg aattcttcgt caaaagagac
     2161 gttgaggtgg tcaagggtat aaccaagacg ttggatcaag atgcttgatc ccatcaggac
     2221 cgtatagtcg acgagtcgtt gtacatcaag ggtctcgatg ttgtttttca ggatgcggat
     2281 gacttcggat agaccaccga tgtatttgaa tttctccagg ctatcgatga tggttttttc
     2341 tttgtctgcc atgttgactg gtgtatcgtc gatggtgaca gggttgaacc cgaagaattt
     2401 tcgtggagta acgtgtatga agtgatagga ggtgtctctt atgtcggtgt tgtttcgtat
     2461 ggtggtggtg acgaggtaga ccggttggtt tattggcggg atgagtttgt gataggatgc
     2521 tgcggtgtga aaagaaaaat agtacgggtc gataagacgg cttccgatga tgagtggatg
     2581 gatcgaggga aggtgtcggt cctgtgttgt tttgaggagg agatactggt tacgtcccag
     2641 ggttgcgaac cattttttca ggctgagtgc aaaaccgagg ctgtgcgctt ctttttctgt
     2701 gcaggcgagg aatgaggcga tttctttggt ggtcacgaac cagtggttgt tctgttcgag
     2761 ccactggatg agtttttttt gtgtctcggt caatcctccc ttcattggat tttggaatag
     2821 atgtacccct tttaatagat ttttatatgc ctgattttgg tatataagtg ataaaagagg
     2881 gaaaaaggtg cggtttttat cttacctgtt tcagtactga tcttatgatt tttttagaga
     2941 gatttttgct agttagaact gccactttcg ggtgtaggtg tcgtattctt ttggatcttt
     3001 atcattgtcg accatgcttt gaacttcgtt gattgctcaa aatcagtagt cgatttcctg
     3061 caccagttat tccatcgaaa tcatggtttt gaagggatta gtaaataaaa gtcatgttgt
     3121 attacagtat tgtactatga aggtgacctg ccctcattgt aaacggcgaa tgcgtatctt
     3181 tcgaaatcgt aagaatgtgt gcgcgtgtgg caaactgctg aattaccgtc attttctgca
     3241 tgagaaaatc gcctatgacg tgtatctggt tgatgccaat atcttgatct atgcatttga
     3301 cagtcgaagt actcgtcgga aatattgtca ggcgattctt gcgatgcatg catcttcgat
     3361 gagcattgcg acgacccggc agatcatcga ggaggttggc tctgcaattg agaagcaact
     3421 gcctgagatg ttcctgattt acaatgtcag tacgattccc ggtgatctca aagagattaa
     3481 aacgaatttt ttgaaacaac catcgattgc tgatctttca ttggtgcagg cagcctacga
     3541 acacccagag gttcgtggga tcatcaccta cgataatgat tttacgcgta tcgcgaccgc
     3601 agggctggtt gagcaaaaag catcgatgaa gttctggttg gggaacgcca aagagtttgt
     3661 ggagaaacat cggataaggt taccggagac agaaggggaa gagggcggat aacaacatag
     3721 atttacaaga gccaagaggc gttttcaaag agtgaatcct atcgataagg atcatgtgat
     3781 tcaacgttct tttctaagag agaccattcc taccagtttg actgctggac cagaatcgca
     3841 ttcttggcac tgctcccatc gtaaggtcat cagatcggtc gtgaaagaac cgaccaacgt
     3901 atccgtacca gcaacgagta aatcaatacg agatgaactg attgatcctt ctactaaata
     3961 acggaaggtc gatccgattt gaacgcaagc aggacttccg tccgtgctta caactgtgag
     4021 atcaaaatat cctgtgacat tggagtcttg ttgttctaat ctcagcttca tcgttccttc
     4081 ataggcacat tcgtatgctc gatcggtaaa cacaggagat cctgtccagt ttcctgtgac
     4141 atccctggcc ggggtgaggt ttgtaccatc gttattctga ttcgtacatc cgctgaattc
     4201 aatcattgtg atgaggagaa ctgctaggac tataatgggt acagtttttt ttcgctgcat
     4261 ttttacaaac acgtagcagt tgttttatta aaaattttat tcagcatttc taccgtactt
     4321 tagtttctgt tttttgtttg aacagaaatt ttttaaaaaa cggtgttgtt ttgaagcagg
     4381 gttgagaatg gaagagaagg aaaggggggt ccggtgatgt taaaaatctg ggaggaaagg
     4441 tttgaaacgt aaaagctttc ttatacggaa agtccagacc cccataatat tatatgatgt
     4501 actactagat aaatgttatc cttgtaaacc catggatatg agaaaaatgt attaaaatcg
     4561 tacatattca actgaaataa gggtactcaa tgtaacattc atcccggaaa gtagtgaaaa
     4621 aacaatgagg attcacataa aaaaggattg acttggaatg aagagaaaag gaggcgacgg
     4681 gttatacgat gccaaaaacg tgagccacgg aatctccgtt tctccattgt ttgtttattg
     4741 tattattctg aggagcctga tgatcttttc ttcattgatc tcttccactg aatacacttt
     4801 gatgtgcttc atggtttttc cgccaccttc gaaaagtcct tgctgccgtg tcgtcaaccc
     4861 tttaagagaa caacccagat tgacatggtt tttgagggcg gcgatgtaat atttaccgca
     4921 ggggtccttt tttgtggaac cataacaagg gacgcctgct ttcatttgtt cgttgatgtc
     4981 aggaaatgtt gtaaggatga tgcttcgtaa tctctgacag atctcttttt gcggggattt
     5041 ttgttttttt atgtattcgt caaccttcgc atccatggaa ataatgacac agcagcatca
     5101 tttaaggttt ccgaaataat tgagaaaagg tggtgttttg agaattttgt tttttgaatt
     5161 tgggacggaa tgtggcacaa aatgggtttg atttgtaaaa aatggatata agcggatctt
     5221 ctcatgcggc tccattgaaa aagacaagga tgtgatcaga cgataaggaa aaaactaatg
     5281 aatcaatgct tcggagcggt tcttgcaagc atggtgcttg cgacgtatcc tgtgtctctg
     5341 gtcggagccc agatgacttc tgccatgaag gatcagatac atcaagatga gattattctg
     5401 cttacggtga aagaactggc tgaccaggga atggatctca accagttaca tagcgaatta
     5461 caaaccatgc atggtagcat gcagtcagat aatcaaaaga ggggacaagg cagtctgtct
     5521 ctaagaggtt tttgtggaaa tagcctcttc tcatgtcgag gttttgctta ggacatcctc
     5581 ttttcatccc tctgcccccg gaaaaggtag ttgtgccatc cttttttcgt cctcccctgt
     5641 gatttgaggc gcaacttcct tcttatccat ttcatggaat tacaatacgg aaatatattt
     5701 aacaatgaat atactatcta tttcaatagg tatcatgtgg gatcaatatg agaacgcgtg
     5761 aattgtttct tggatgtggc atcgtgcttc tggtcgtgtg cctctgtgct tcttcagttg
     5821 gtgcactctc tcattcacct gatcaacctg ggccatacca tatcggttcg tacaaggtgt
     5881 ggtatctaga acctccgtat ggaatatacc gggcgacaat ccggtatcca gccacctcag
     5941 acggacagcg tgcgcctgtc gattcctcag cagcaccatg ctcaggtatc gtggtcgcga
     6001 atggttatgc tggatctgaa tggcagatca cctggatccc agagcatctc acctcctatg
     6061 gctatgtgac gttgtgtttc accccgcctc ataggctctc ttgggatacg acgcaatggg
     6121 cgtctgggtt tctcggtggg ttgagggtgt tacagaatca gaacgatcgt cggttttcac
     6181 ctatttatca tgttctggat ggggagacat ggggcgcgat cggtctttcc atgggtggtg
     6241 gcggatgtgt tgaggcgacc gggactactg gctcagaaat tgatgcgtca gtcgcgcttg
     6301 cgcccgcagg gtttccacca gtgctagctg ctgcgcggaa catcacggtg ccgctccaac
     6361 ttcaggtggg gacggtcgac agactggttc ctccagatcg tgtgctggat atttacagta
     6421 attaccttac gaatggtaca accaaagaat atttggcgat cacagggggg aaccatatcg
     6481 ggttcatcga cgagaagtat gcatcgtttg gtcaacgatg gaacatcgac aatccaccag
     6541 gtatcagcgt cgaacagcaa cataatatct cacggacata cttcaccgca tggttccagt
     6601 accatctcag acatcttgat gagtactata cctatatttt tggagaggaa gcacaacagg
     6661 atctggacga tggaatcctt gctgatttgc gatataatat cccgtagaag atttactgtg
     6721 aattaatgga aaataagatg tggtttattt tatgccgagg aggaagaaaa agaagatgaa
     6781 tgcttgtttg ctgcttgtct cgttgacgcc ctgatcacag gatatggaaa gggtaatcgt
     6841 tgttttcccg aacccaataa tcatccccaa tcgtgcttct tttgtttctc cgacagggat
     6901 actagggaga atgactgttt tgttttttcc catgaaaaaa aaaccaccgt ctaagcgtct
     6961 gctgataaaa acattggttg cgttcgcatc cccgatgttc ctcacaaacg tcgtcgaccc
     7021 tttgaaagtg atttcgaact gggggtgcgg tttccagaac acatatgcag atccagttga
     7081 attattataa ttgcaatttc caacgattac acatgtatcg gtgcttgaaa tcatattgct
     7141 tagtcctgaa aattgattgg tgaatacggt cagtttcgca tcttgtgacc aggtggttcc
     7201 attccgcttg aagatgtaga cggacccatt atgttcgaaa aatgacatac caattgtcgt
     7261 tgcgtaattt tcagatatag aaacagccca accgaataac tccagaggat tgccatccga
     7321 tcctctcagt tgtgcctgct gtgtccaagt ggttccattg tgtttgaaaa tatatgcagc
     7381 tccggttaag aaatcgaagc acggtgctcc aatcataata tgatcgccgt ggatcgaaac
     7441 agatgtacca aatccagtcg gttcgctatc cggtgagact agttttgctt cttgcgtcca
     7501 ggtcgttccg taacgttcga agacatacgc cgactccgtc gaattctcac caccctgcat
     7561 attaatacct atgacaatgt ggttcccgtt tattgaaaca gaatttccaa agtgatcgtc
     7621 aggtttacca tctgatgcaa gcagtttggc ctgttgtgtc caggttgtac ccttccgttc
     7681 gaagacatat gcagaccctg tcgaattatc atcgaggcat gcgccaataa cggcataatt
     7741 ctcacttatt gaaacagaat taccaaaaga atcgataggt gcaccatctg atgccagcag
     7801 cttcgcctgt tgcatccacg ccgtatctga gttggtgaag atgtatgccg atccagatct
     7861 atttccattg tcatcatcct ctatcgctcc gataacacac gtgtttccat cgagggagac
     7921 cgaccaaccg aaacaatctt cgggtacgct gtctgctgct gtgagttttg cttcttggat
     7981 ccaggtagtt ccgatgtgtt taaagatgta cgcagctccg gtaatattat cattaaatgg
     8041 tgctccgatg agagcatagt tcccagagac tgaaacagaa gaaccgaagc tgtcggaggc
     8101 ttctccatct gaagcgttca gcatcatcat cttccagtca gaggatgtac tcataaaatt
     8161 tttttgttga atcgtagatg gatgtgactc aataataatt tctttctcca gtccattagt
     8221 actcttagcc tttgtcacag aaacgagagg actgatgaga agcattacgg aaagaagaat
     8281 aacaaatata ctatttttca acgtgtttcc tccatattat tgatcattag ttcccctcat
     8341 taatgttatt catcgggata ttatggaatt catggaataa actgatggca ttattcagaa
     8401 ttccgggaga tgccggtgat gtggaaatgt aaagggaata gttcagcctt ctttcttata
     8461 agaccaaacc gtagcctact actgcgaaaa aatgtgagcc gatgagtcct ttgaactcat
     8521 cattaagaag taagacttgt atcgaagagt acagtttcaa acctattggc ttcagtttta
     8581 tgacgagtgc aattgacatg ttaaggatgg tacagttttc acattgattt ccagcacttt
     8641 gaacaaatcc aatgaggatt attttagaag taatgtagat agtaaatggc tctgaccagt
     8701 cgctttctac cccgaacggg tcctttacct tcagtcggaa gttataggtg ccaggttcag
     8761 accatcgatg ggttaccgtg aaagtctgat cggagggata cggaccaagc catccgctgt
     8821 tgtttccatc gccccattcc aagaaaaagt aatataggtc gccctcggga tcgatactcg
     8881 cattaatggt aaatgtgtaa ttcgtattta tttttccgca atgcggacca ttaattgttg
     8941 gtgggtttgg tggtgtcagg atatcagtgt gaagagagat agctgttggt gcaaccaaga
     9001 cgagaaccag catacaaatt aagagatagt tacgtttctt tatcatgaaa ttcttccctc
     9061 gctttaattt tatgcctatt aatatgatat ggactgattt aaatgttttg gggagataag
     9121 aattggataa ctgatgatcc gtttcaatgg ttttattaat ttgaaaggta aaagcattct
     9181 cagaaagtca acgctgggct tggttttcac tattctgttt tccgctaaaa gaatgggaat
     9241 aaaaaaggaa aacgcggttt catcgggggg atctattgct gagggaatgc ttctccggac
     9301 cagtctgacc agccagttcc tgcgattggt acggtcattc cagtatctgc ctcatagaac
     9361 gtcccggtga agttcgcgat aatctcaaag gtgaccgtgt cgtaaaaagg atctggaacg
     9421 tcgaagtaca cgatcatgta tcggaggagc gaccattgta cgattaattt gttaccgctg
     9481 tcgtcttctg cggtgatggt tgcctgtgtt ggatacttga catcggggta tcgcgggtcg
     9541 gtcgcaaggt ttccgtactg tacattgatt tcatttttcg cgaggtcgca tccgcggtag
     9601 aaattgtatt ctttctgttc cccggtggtg tttgtgacga cccggaggtt ccctggtgtg
     9661 caggtctccc atccctcctc gcaggagtac acgggcgcat gcgggtagag gagatggacg
     9721 gagagattgt cctgtttgaa gtcgatccag ttccattcaa atatcgagaa gacgaactcg
     9781 ctgtagaacc catcatggta tcctttggca tcaacaagat tatatgttgt ctggtttgcc
     9841 ccatccttga cagtgtagat cccgttgacg actgcgtttg ccatttgcat atggtaggtg
     9901 atccatgccg ggatgatgcc gagggtttta ggaacaggcc aatgcaccca tggaagccat
     9961 ggttgtccaa ggcttcgtct gtaatggagg tcccacgtga tttctttgct gtctttaata
    10021 acctggccgg tgatgtgata atgatccggg gagatgacat cgatggtccc gcttgggttc
    10081 tgcaactcag gtccgggtcc tggttcgaag gtcggggaag cagaaaagtt acttgggttg
    10141 gtgggatcgt aattgtcgct gaaaagaggg aaggatgtcc cttccgatga gttccgtatg
    10201 atcatcccaa acgtgttggt aaggcctccc ccggtaagtt tgttttccgg gttcgcaaga
    10261 ccgtaggccg caacccctgc gatgtcatct gaggtgtcgc tggtgcctcg atcgtcgagg
    10321 aaattgaagt accagagctg gttgtagacg aaatccccca cgccattctt attcctctga
    10381 taattatagc cgtcggtgtt gttctggttc gctgtgccga ccttgggggt agaaggctct
    10441 tcagggaggc ggccaaggca ccatatattg atattaaatt ctgtggatac attagtacca
    10501 tagacatcgt acgttacaat tctaatttta tgtctaaaga agagtggatc gtccaacatc
    10561 cattcgtagt agggtccttg gagacgaacc ttaaatatat tatcaacata gatatcgata
    10621 gacattattt cggatgaatt atcagcagct tcaagcacca tggtgagctt accgataatc
    10681 tgtatcctcc ccagaggcaa gcgtgctact tgcttatctt gaatatagag agacccaggt
    10741 tttggtttga ctattattat cattggactt gtttgatttt ttagagttgg ttcaaaggta
    10801 tctgatcttg tatggagttg ttgcccggtt gcgctaaaaa gattcatcat gaggaggagg
    10861 atgactatac atccggttcg gaggattatt ttcttgttca aattcatacg attccccttg
    10921 ttttaagaag aggaactggt ttataatgat tgtcatcagt gaacatgtca cattacgctg
    10981 agaagaaaaa aagatttcct gttgtatcct gggagaagaa aagagaaaaa ggtatctttt
    11041 taagggaata aaccatgtga atatcgcgag gcgtggagat gggaccgtga gaaaaataat
    11101 tgccgtactc attggactta ttctgattat ggtatgcgcg atgagcggat gtacagaaaa
    11161 taaaccaacg aacaccgggg caacagacac ggatggtgat gggtacagtg acgctgtcga
    11221 tgcgttcccc tctgacagta ctgaatgggt tgattctgat ggtgatggag ttggggacaa
    11281 cagcgatgcg ttcccacagg atgcgaacga aacacgggac acggatggcg acggggttgg
    11341 tgataacaat gatgcgtttc cgactgatcc aacggaatca gttgactctg atggcgatgg
    11401 cgtgggtgat aacgcggatt attaccctga tgatccaaca cgatggaagc agccttcatc
    11461 tgatgatttc caaaagcaaa ccgagccgtt tctcttaaaa ctgaacctgg aggatagtga
    11521 gttgcattct tatgcgaata ccatccttac cggctgcgat gcgtccagca tggaatgttt
    11581 cgtgaacacg ctgtaccgcg atgtcctgat gaactattcc tgtcttgcaa ctacgatgga
    11641 tgctgagaca ttgcaaacgc cacgggagac catcgagaaa aaagaaggga cctgtgagga
    11701 tctgtctatt ctcctatgct cgttgctttc gaatatcgga ttgaccacgt acctcgtctt
    11761 taccgatacg catgtgtatg cgatggtctc tgatgtcaac gccgatgatc tctggcatgt
    11821 tgctgagcag tcattgattg gtcatgtcga ggacattttc ggggagccta tcatgcaatc
    11881 ctattatcag acctatgaac taccgtccat gggtgtactg tatgctggtg gcgaggaagg
    11941 aaaaacattc gcaggtgtca tcgactcaat gactattgat tatactatcc aatccgatca
    12001 gcagttgact ctgtttgttg tcccaacatg gactgagtac tctgagttcc agagcgggga
    12061 ttttgcgaac tttacgccca gcgatcaatg gaattttacg agtaccattg ggtcgatccc
    12121 tgagatgtct acgtttggcg gtatcatgct ttacaatcca agtatgcaga ctgcaacggt
    12181 gactgtcgat cttctctttt catttcaagc atcgttttac aagacgtaca atcaggatgc
    12241 gttgacggtc tatagtgtct gggggaagga tgcggttctc ctggatccaa cactggggga
    12301 ttttgggttc ccaggctatg acgtcacggt cgttggagtg aagaaagcga tcgatccagt
    12361 gacgaaagca tacgtcactc tgtcgtaaaa gagaaacgcc ccacggagtc gatgatcctg
    12421 cttattttct tagtgattgt gaagagatca acgttcgatg gtaatattta tatattattg
    12481 ctgcgtaata atatggacgg tttgaagaga tggagatggt gcgtataaaa gaaaaagatg
    12541 aagcagataa acaagcaagg gagcggctgg agaacggtac attaaaatca ttacgatcct
    12601 ggtataaaat actcatagag gaattggaac aaactgaaga gtattataat atcacaattt
    12661 atcaagtgcc tccgtacccg ccaaacacca aggaagcaaa ggattttttc atacaaagga
    12721 cttaaaatac cgttgttgag aacactggca gggttcttga atttgtaccc tacaaatcgt
    12781 taacttttat tgttttggta aaaatgtagg ggtgattatt ggagatgcag ggattgagcg
    12841 gattcgtgct tgaatcacag aaaatggttt tacagcgaga tgaggtagat gccgaggaac
    12901 acgatgaggg tgccgataaa tcggtgaggg taagattttt ctttgaggaa taaggttgtc
    12961 agaaggaaaa ttgtgatgat gctcactcct gccaggggat agactttgga agcgttcgcg
    13021 tcagataatg cggtgtacag gagtactgca gcggaagctg ccaacaggga tgcgacaaga
    13081 acagaacgtg cgcgaggtct gacgtaggat ggtgctggtg gagtgaatcg tcgcattatc
    13141 agaagcagga ctccaagtat gaggaacgcc atcagctgga tagagactgc caaatcgata
    13201 ggttctgtgt tcccgaggaa ccatttgatg aggagcgcag aggcaacatc aagggggata
    13261 agcgcagcga tgtacacgaa cgaacgatcc agtcggggta ccgtgaagat gcgtacggca
    13321 aggacaaggt agattccgca gagtatcaaa aggattccga tgtagttttc ccatatcact
    13381 gtttcttgga ggaaaaatat agaaccaagg aaaatga
//
LOCUS       DATMXJ010000194         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50846, whole genome shotgun sequence.
ACCESSION   DATMXJ010000194 DATMXJ010000000
VERSION     DATMXJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50846"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1126)
                     /gene="mvk"
                     /locus_tag="VN377_05140"
     CDS             complement(173..1126)
                     /gene="mvk"
                     /locus_tag="VN377_05140"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HWR27803.1"
                     /translation="MKKGHGIGFGKAILFNEHFVVYGIPSIVSAIGNYTVAKIEPYDH
                     AGFTLSDKRPATPKYKEDKLSQQKESFELMFKKMGLDLSKKGVNITLEGNLYAASGIG
                     ASAASCVAVARALAEYFEMNLSDDEINEIAFEGEKGYHGTPSGVDNTASTYGGLIWFQ
                     KGEKNIIDRIALSSPVEIVIGNTGKVTDTKVAVEGVKQRKEKNPKKYQEVFDRAENIA
                     YLAKRAIHDGSLQDVGKLMNENHKLLQQIEVSSRELDFLVSIAREAGAMGAKLTGGGL
                     GGNMLALTPGKELQNKVATAIEKEGFQTVKTVIGASRESVE"
     gene            1607..3949
                     /locus_tag="VN377_05145"
     CDS             1607..3949
                     /locus_tag="VN377_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27804.1"
                     /translation="MVRNNPIVIGLLIGVILFIIGVGTLCVKGLSECTIRLTESQTTL
                     PQGHPNFEPFLAHRIKEIESHYLLLNSWSFLSKKDDGRLRLGQLTYTEGTQISSPSSF
                     IERSGNYTPHDPIWINGDNQFTSDNGVVGGSGTIDDPYRIEGWEINGSEDSISIWNTR
                     SYFLVANCLLEGSVGLNLVDVTNGTVQSNFLTHGLSIHDSIYITINNNTPQGVALDNV
                     TYTTITNCSGRIYSQGSSYLTLSNLEIIGYEMGIEFYNTNHTILRNCTVSSNNYGILF
                     YNSPFLYLRDNRIYNNTINLNIEGVKNEDFYHDIDASNTINGKPISYLYNAKNLVFDE
                     TTDIGFFGFVNCTNITIKNIVLTNCGVGVLFVATQNSSIISCEFSDTLHAVGLFCGSQ
                     NNVIFNCTSFDAEIYLYGSPNNLLRNNSLYNFWVEGNSLSDFYQDIDSSNTIGGRPIY
                     YLVGKKNMIFRESDVGFLALIDCENILVSNIVNANQGLLVVRSRVIIRHCDFNHHTYG
                     IFITNNSQTWICESKISNNIIGIMFNRSSSTTVLRSNITENLVGSHFEKSNNIIIRNC
                     DIISNAFSGIQIANSWNNILCLNNISDNGMGITMWGDCYNNEFCDNRIWNEYQGFEMR
                     PDYDIDPSLGPHDNTIHHNSISFIPYWGFFLVNSERNTIHHNSISNCKYAMDFFYCGL
                     NTVQQNNIFNNEEGIYASKSTIDAEKNWWGAKDGPSGTGPGSGDSIRTNDDSNITFEP
                     WLNHPAITKPSSFRSLLTCILARVILLDRLGKGFEPTSTG"
BASE COUNT         1188 a          897 c          760 g         1188 t
ORIGIN      
        1 attcccccga aaacagggaa cggatatcct ttcacctttt caaagatagc tggtttctca
       61 ttcaaagtgt acatgatatt agcaatctca tattccaccg agacttcctt ttttatccga
      121 actaacttcc cctgctcttc cagctgttgg agaaacggac gaagattcat acttactcca
      181 cactctcccg tgatgcgcca atcacggttt tcacggtctg aaaaccttct ttctcaatag
      241 cggtcgcaac cttattctgc agttcttttc caggggtcaa cgcaagcatg tttcccccca
      301 gaccaccacc agtcaacttc gcacccatgg caccagcctc acgagcgata ctgacaagaa
      361 aatcaagctc acgagacgac acctcgatct gctggagcag cttatgattc tcattcatga
      421 gttttccgac atcctgcaat gatccatcat ggatcgcccg tttcgcaaga tacgcgatgt
      481 tctccgcccg gtcaaaaacc tcctggtact tcttcgggtt tttttcctta cgctgtttga
      541 ctccttcgac cgctactttc gtatcggtga ccttcccagt gtttccgatc acaatctcta
      601 cagggctgga tagcgctatc cgatcgatga tgttcttctc acctttctgg aaccagatta
      661 acccgccata ggtcgacgcg gtgttatcca caccagatgg tgtcccatga tacccttttt
      721 cgccctcgaa agcgatctca ttgatttcat catcagaaag attcatctca aaatattctg
      781 caagcgcacg cgcgaccgcg acacaggatg cagcagacgc accaatgccg cttgcggcat
      841 acagatttcc ctcaagtgtg atatttactc ctttcttgga gagatccaaa cccattttct
      901 taaacatcaa ctcaaaggat tccttctgtt gcgaaagctt atcctccttg tatttcggtg
      961 tcgctggacg tttatctgaa agtgtgaatc ctgcatggtc atacggttcg atttttgcga
     1021 ccgtatagtt cccaatcgca gatacaattg atggaatccc atacacgaca aagtgttcat
     1081 taaataatat tgccttcccg aaaccgatgc catgaccttt cttcatacaa tatcccatag
     1141 cgttctcaga tggattggtt acaatacaag ataagtatat tattcttacc gtgaaatgcc
     1201 atggaaatat aaatgtgcgg ggacgttgga ttcgaaaacg gaactactga ttttcatccc
     1261 ctgtcggaaa gaacaatcct tcgatattta aacctttgtc gagagaaaaa aatgcagcgg
     1321 acgagacttc aacttcaaat ctcattctta ctctggaatt taatccttat tcttctaata
     1381 aaacagaagc ggaaagggag attcggattc atatttgtta acaaaattga cagtataata
     1441 tctgttgctg gagttttttt attaaataag aatgatgggg ctagggaaga caatacgcca
     1501 aatgatataa atctatactt gagagtctta atcctgagaa aaatactaca gataagacgt
     1561 aacaaactat taatatgata aacatcttgt taatatgggg gaatatatgg ttagaaataa
     1621 tccaattgta ataggtctgt taatcggagt tatcctgttt ataattggag taggtaccct
     1681 ctgcgttaaa ggactgtctg aatgcacgat tcgcttaacc gagagccaaa caacattacc
     1741 acagggtcat ccgaacttcg aaccattcct agctcatcga atcaaagaga ttgaatcgca
     1801 ttatcttcta ctgaactcat ggtcattttt atctaaaaaa gatgatggtc gattacgact
     1861 tggacaatta acatatactg aagggacaca aatttcttca ccgtcatcat ttattgaaag
     1921 atctgggaac tatacacccc atgaccccat ctggattaac ggagacaatc aatttacttc
     1981 agataacgga gtggtcggtg gaagcgggac tattgatgat ccgtatcgta ttgaaggatg
     2041 ggagattaac ggctctgaag atagtatttc catttggaat actaggtctt attttctcgt
     2101 cgccaactgc ctcttagagg gatccgtagg tctgaacttg gtggatgtca ctaacggaac
     2161 cgtccaatcg aatttcttga cgcacggact aagcatccat gactctatat acattacgat
     2221 aaataacaat acgcctcaag gagtagctct tgacaatgta acatatacga cgataactaa
     2281 ctgtagcggt agaatctata gtcagggctc ttcatacctg acgctttcta atctggaaat
     2341 cataggctat gaaatgggga ttgaattcta taataccaat catacaatcc ttcgtaattg
     2401 taccgtgtcc tcaaataatt acgggatttt attttacaat tcgccctttt tatacctgag
     2461 agataatcgg atttataata acacaattaa cctcaatatc gaaggagtga aaaacgaaga
     2521 tttttaccac gatatcgatg catcgaatac catcaatggg aaaccaatat cctatctcta
     2581 caatgcaaag aacctcgttt ttgatgaaac aacggacatc ggttttttcg gattcgtcaa
     2641 ttgcaccaac attactatta aaaatattgt tcttacgaac tgcggtgtcg gagtcttgtt
     2701 cgttgcaaca caaaactcat cgattatctc ctgtgagttc tcagacacac tccatgctgt
     2761 tggactcttc tgcggctcac agaacaatgt tattttcaac tgcacaagtt tcgatgctga
     2821 aatttactta tatggttccc caaacaatct cctccggaat aatagtttgt ataatttttg
     2881 ggtcgagggc aatagccttt cggatttcta ccaggatatc gattcttcga atacgatagg
     2941 tggaagacct atctactatc ttgttggcaa gaagaacatg atcttcaggg aatccgatgt
     3001 tggtttctta gcacttatcg attgtgaaaa catccttgta tcaaatatcg taaatgcgaa
     3061 ccaaggtctc ctggttgttc gttcgagagt aatcatacga cactgtgatt tcaatcatca
     3121 tacatatggg attttcataa ccaacaattc tcaaacgtgg atctgtgaat caaagatctc
     3181 caacaatatc ataggtatca tgtttaatcg ttcctcgtcc accactgttc tccgaagtaa
     3241 catcacagag aacctcgttg gaagtcattt cgaaaaatcc aataacatca tcattcgtaa
     3301 ctgcgatata atctctaacg cctttagcgg aatacaaatc gcgaattcat ggaacaatat
     3361 cctctgcctc aataacattt cagacaatgg catgggtatt acaatgtggg gggactgcta
     3421 caacaatgag ttttgtgaca acagaatatg gaatgagtat caaggctttg agatgcgccc
     3481 agattatgac attgatcctt cccttggccc gcatgataac acaatccatc ataacagtat
     3541 ttctttcatc ccctattggg gattcttttt ggtaaattca gaacgaaata caatccatca
     3601 caacagcatc tccaactgta aatatgccat ggatttcttt tattgcggtc taaacactgt
     3661 tcagcagaat aacattttca ataatgaaga agggatttat gcatcgaaaa gcacgatcga
     3721 tgcagaaaaa aattggtggg gagccaaaga tggacccagt ggcaccggtc caggttccgg
     3781 tgatagcatc cgtactaatg atgattctaa tatcacgttt gaaccctggt taaatcatcc
     3841 tgcaataaca aagccttctt ctttccgaag cctgcttacg tgcattctgg ctcgtgtgat
     3901 attgcttgat aggctgggga agggatttga acccacgtct accggttaaa ttctgagtag
     3961 gggataataa ttactcctgt aaaagacttc aatttcagat gttttttaag atcattcctg
     4021 ggatgtcata ggg
//
LOCUS       DATMXJ010000195         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50871, whole genome shotgun sequence.
ACCESSION   DATMXJ010000195 DATMXJ010000000
VERSION     DATMXJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50871"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VN377_05150"
     CDS             complement(<1..1388)
                     /locus_tag="VN377_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWR27805.1"
                     /translation="MKDNKTLLESSYDSDKIQVLEGLESVRKNPGMYIGSTDARGLHH
                     LVYEVVDNSIDEALAGYCTSVQVIVHKNGALSVADNGRGIPVDIHEKYKIPGVEVILT
                     MLYSGGKFDGQAYKVSGGLHGVGVSCVNALSKWLEVKVRRNEKEYQQRFERGKTVTDL
                     KVIGKAKDTGTTVTFYPDKEIFETIEFDYDTIATRLREMAFLNAGLQIEITDERTEKH
                     EVFRYDGGVSEFVLHINKNKIPLHPKPIYLQGEKDSVQVEIAMQYTDGYTENIHTFAN
                     NINTIEGGTHLAGFKGALTRVLNDYGKANKLFDESFSLSGEDSREGITAIISVKIRKP
                     QFEGQTKSKLGNSEVRGIVESVTCDKLAEFFEENPPVAHMIIDKAVLSSKAREAARKA
                     RELTRRKGLLESSALPGKLADCSTRDPSQSEIYLVEGDSAGGSAKQARDKEFQAILPL
                     RGKILNVEKARM"
     gene            1706..>2356
                     /locus_tag="VN377_05155"
     CDS             1706..>2356
                     /locus_tag="VN377_05155"
                     /inference="COORDINATES: protein motif:HMM:NF017874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vps62-related protein"
                     /protein_id="HWR27806.1"
                     /translation="MNKKIIISIFFIGILLAGSNFVSIATAADQDVAEQYAPILYFVD
                     GEQCFPVDVSYALDNSYLYENGNPTPLSITPTADILKNKTTDNYYLDNQRGTVAVGDN
                     GIENDYQSKMAALGYTVYAHVDTQNNVIQYWFFYAFNGGDLNRHEGDWEMIQVVLTGG
                     QPTEVMFSQHYSGQKAKWSQVDKEGAHVKVYVARGSHANYIKPYSGKIGLASDTVVD"
BASE COUNT          602 a          493 c          528 g          733 t
ORIGIN      
        1 tccatcctgg ctttctcgac gttgaggatc tttcctcgga ggggtaggat cgcttggaat
       61 tctttgtctc tggcttgctt tgccgagcca ccggctgaat ctccttccac aagatagatc
      121 tcggattgac ttgggtcgcg agtcgaacaa tcagcaagtt tccctgggag agcagatgat
      181 tcaagaagtc cttttcgtcg tgtgagttct cgtgcttttc gtgccgcctc acgagccttg
      241 ctggaaagca ctgctttatc aatgatcata tgagcaacgg gtgggttttc ctcaaagaac
      301 tccgcaagtt tatcgcaagt gacactttct acgatgcctc gtacctctga gttacccagt
      361 tttgatttgg tttgtccttc aaattgaggt tttcttattt tcactgagat aatcgcggtg
      421 attccttctc gtgaatcctc accgctaagg ctaaagcttt catcaaagag tttatttgcc
      481 ttaccataat cattcagaac tcgggttaac gctcctttga atccagcaag atgtgtccca
      541 ccttcaatgg tgttgatgtt attggcaaag gtgtggatgt tttcggtgta tccatcggta
      601 tattgcatgg cgatttctac ttgcacgctg tctttttcac cttgtagata gattggtttc
      661 ggatgcagag gtattttatt cttgttgatg tgcaggacga attcgctgac cccgccgtcg
      721 tagcggaaca cctcatgttt ctcggtgcgt tcatcggtta tttcgatctg cagtcctgcg
      781 ttgagaaacg ccatttcccg caggcgggtc gcgatcgtgt catagtcaaa ttcgatggtc
      841 tcgaaaatct ctttatctgg gtaaaatgtc accgtggttc ccgtgtcttt tgcttttcct
      901 atgactttga ggtcagtgac tgtctttcct cgttcaaagc gttgctggta ttctttttcg
      961 tttcgtctga ctttgacttc aagccatttt gataaagcat tgacgcaaga aacaccgact
     1021 ccgtgtaatc cacctgagac tttgtatgct tgtccgtcga atttaccacc tgaatatagc
     1081 atggtgagga taacttcgac gcctggtatt ttgtattttt catggatgtc gactgggatt
     1141 cctcgtccgt tatcagctac ggagagcgcc ccgtttttat gaacgatgac ctgaacagat
     1201 gtacagtagc cggcgagtgc ttcgtctatg gaattgtcca cgacttcgta gacgaggtga
     1261 tgtaatcctc ttgcatcggt gcttccgatg tacatccctg ggttttttcg gacggattcg
     1321 agtccttcga ggacctggat tttatcagaa tcataggagc tttcaagtaa ggttttgttg
     1381 tctttcatga agatcacttc gtgacaaagt ctacactagt tccggttgtt ttatgttaga
     1441 tgtttttatc ccattctcct agggtagctt tacaggagac tcatatggag taaagcctaa
     1501 taaaggttac tattgagcga accttaataa tttctaatct catcattcct atataaaaac
     1561 catagcttca ttatgtatag tatgctgctt aacaatagaa aagttaaaaa gagtatttaa
     1621 acatataaaa agaaaaaggg attttgaccg gttttttaag aaggattaaa taacgacaat
     1681 tctatccaca tcaaggtgga ggcctatgaa taaaaaaatc atcataagta tattttttat
     1741 aggaatttta ctagctggaa gcaatttcgt cagcattgca acagcagcag atcaagatgt
     1801 cgcagaacaa tatgctccta ttctctattt tgtggatggt gaacaatgtt ttcctgtcga
     1861 tgtttcctat gctcttgata actcgtattt atatgaaaat gggaacccaa cacctttgtc
     1921 gataacacca accgcagaca tacttaaaaa taaaacaact gataattatt atcttgataa
     1981 tcaacgaggg acggtggctg tcggcgataa tggaatcgag aacgattatc aaagtaaaat
     2041 ggcagcatta ggctacaccg tgtatgctca tgtcgatacc cagaataacg tcattcaata
     2101 ctggtttttc tatgcgttta atggaggtga cctgaaccgc catgaaggcg actgggagat
     2161 gatccaggtg gttctcactg gtggacaacc gacagaggtc atgttcagcc agcactattc
     2221 agggcaaaaa gcaaaatgga gccaggtcga caaggaagga gcgcatgtca aggtctatgt
     2281 ggcgcgcgga agccacgcga actacatcaa accatattct ggtaaaatcg gtttagcaag
     2341 tgacacggtt gtagat
//
LOCUS       DATMXJ010000196         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_24296, whole genome shotgun sequence.
ACCESSION   DATMXJ010000196 DATMXJ010000000
VERSION     DATMXJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24296"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..3475)
                     /locus_tag="VN377_05160"
     CDS             complement(68..3475)
                     /locus_tag="VN377_05160"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR27807.1"
                     /translation="MRNKIIGIVVFMLLLTSSTAVVVAWNFAPEQKQNDANEPLGRDY
                     REYGDAPEGPTHIAYPATGVMGAFPTCMGCGPASWIQHNNFGAWFGPTFDFEWEGNAG
                     WCPGGFPPYDQDEGYQDGDAGLIIPQPFTINVAMNVVPFPGWAGSALGSPGQNAVWGV
                     NVDIWVHNTMPNHEPYLPAFVNVLMDWNQNGAWGDAGEHVLVNFVVPPLYIGPLSALI
                     PPNFVIGPNSGYVWTRFSITEAAVPYNWIGEGSFEDGETEDYLLLVNQSTEGGWYYKP
                     AYANYAPQGMPDFNQTQDQWKSIVDGGNGVANTAALGDDIQVVPVGGAVSPGAVVVAP
                     GPNCALNTNPAGDDVAKWAFCGPVAVANCFWWFDSKFDDPNGYPGDGVDNFSLVQKYG
                     VTDDHLKTNVPPLIQDLAAKMNTTTKGTTNITDMKNAIQTWLINKGLSPVFTVNKYNA
                     PNFSFIEDEIERCQDVILLLGFYNESEKLVDQMQMLFSTNDNLQPQNWWDYQSFVPSV
                     NKLDAINITLVSNGPPCDVQINVYNAAGQLVGTSIMNPGALAAPTWVQFHFPSTILLN
                     IGSTYYFDVREVPTPDLNHYEWFYFAGFNNYPPGQGWMNGVVFNYDWAFRTEYYPETS
                     RLKGHFVTCAGVNSNLSKIAISDPELNIQNPAGNHNDAANVSHDIYSVGYGSPLQTLP
                     YQWWLQNYSSVYDYAIVEQAVIVCPRPYNFPPTFGTPTPANGSTNNPRSFTWSIPIND
                     PEGNPFSWTIQCSNGQTNGGAGAANGTKTLALSGLAYATTYKVWVNATDPPSGSGIYN
                     RKWYTFTTKTSLPPVFGTPSPANGSTNNPLSFSWSISINDLDGDTFSWTIQCSNGQTN
                     SGSGSTNGTKTLSLSGLAYSSTYKVWVNATDPGGSGVYTRRWYTFSTIVNLPPVFGSV
                     TPSNGSANNPLSFTWSIPINDPEGNPFSWTIQCSNGQTNSGTGASNGTKNLALSGLAY
                     ATTYKVWVNATDPTGSGQYTRKWFTFSTQQQQNSPPNKPNKPTGPPSGKPNTAYTYST
                     STTDPNGDQVYYQWDWGDGSQSNWLGPYNSGAKINTTHTWGKGSYSIKVKAKDSYGAE
                     SNWSDPLTITMPKNNAFNPLYTFLQMIKQFFDQFPHAFPLLRSLLGW"
     gene            complement(3625..4323)
                     /locus_tag="VN377_05165"
     CDS             complement(3625..4323)
                     /locus_tag="VN377_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR27808.1"
                     /translation="MISVRNLTKRYGKITALNNVSLQFNDVNVIAIMGENGAGKSTLL
                     KIIAGILPFDSGVVMIDSYSIVTQSLLARQHLGYLPEIPELYDRITGREFLFYIASLR
                     KLINAERIITDLSQMIGITPSLDYELGGYSKGMKQKISLLSAIMHQPQNLLLDEPVYG
                     LDPLASRTIQDFIKKRKGTTVLATHSTQLVEQVADSVYFLMNGNVIFHDTVFNLMKQH
                     GTIENAYFHYKDKK"
     gene            complement(4381..>4781)
                     /locus_tag="VN377_05170"
     CDS             complement(4381..>4781)
                     /locus_tag="VN377_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWR27809.1"
                     /translation="YTIAGKKLLQRYSALSLTVYAFLFGSLGLLPFLSSSLITETTAL
                     SWAGWGAVLFLALLPTVIGYILWYVALEIKTASEISVFLYFIPVLSTIISYFLFQELI
                     TWLFIFGGALVIGGLIIVNMKKSYSKRTKS"
BASE COUNT         1331 a         1045 c         1077 g         1328 t
ORIGIN      
        1 gtttcagacc acgcagtatt cccagtcgca actataaaaa aaaaaaaagg aaaaagagga
       61 tggtttgtta ccatcccaat agagaccgca agagcggaaa tgcatggggg aactggtcga
      121 agaactgctt tatcatctgt aaaaatgtgt acaacgggtt aaatgcattg ttttttggca
      181 tagtgatagt caatgggtct gaccagttgc tctcagcacc atatgaatcc tttgctttga
      241 ctttgatgct ataggatcct tttccccatg tatgtgttgt gttgatcttg gcaccagaat
      301 tataaggtcc gagccaattg ctctgggatc cgtcacccca atcccattga taatacacct
      361 ggtcaccatt tggatcagtg gtactcgtcg aataggtgta cgcggtattg ggttttcctg
      421 aaggaggccc tgtgggttta tttggtttgt tgggtgggga gttttgttgc tgttgtgtgg
      481 aaaacgtaaa ccatttcctg gtatattgtc cactccctgt tggatcggtc gcattcaccc
      541 agactttata ggttgtggca tacgcaagac cagaaagtgc caggtttttt gttccattcg
      601 atgctccggt cccactattg gtctgtccat tgctgcattg gatcgtccat gagaacggat
      661 taccttcagg gtcattgatc gggatgctcc aggtgaagct cagagggtta ttggcggagc
      721 cgttagaggg ggttactgat ccgaaaacag gcggcagatt tactatcgta gagaaggtat
      781 accatcttct ggtgtacaca ccactgccgc cgggatctgt cgcattcacc cagaccttgt
      841 aagtacttga gtacgcaaga ccagaaagac tgagtgtttt cgtcccattt gtcgaaccac
      901 taccgctatt ggtttgtcca ttgctgcatt ggattgtcca tgaaaacgta tcaccgtcaa
      961 ggtcatttat cgagatactc caactaaaac tgagaggatt attagtagat ccattagcag
     1021 gagagggcgt tccaaacact ggtgggagac ttgtttttgt cgtaaacgta taccattttc
     1081 tattgtagat cccgctacca ctagggggat cggtcgcatt cacccagact ttataggttg
     1141 tggcatacgc aagaccagaa agcgccaggg ttttcgttcc attggctgca ccagccccac
     1201 cattagtctg tccattgctg cattggatcg tccatgagaa cggattacct tcagggtcat
     1261 tgatcgggat gctccaggtg aaacttcgtg gattatttgt ggaaccgtta gcaggggtag
     1321 gtgttccaaa tgtaggtggg aaattatagg ggcgaggaca tacgatgacc gcctgttcga
     1381 caatcgcgta atcatataca gacgaataat tctgtaacca ccattggtaa ggaagtgttt
     1441 gaagtggaga cccatagcca acgctataaa tatcatggga gacatttgct gcatcattat
     1501 ggttcccggc cgggttttga atattgagct caggatcact gatcgcgatt ttcgacagat
     1561 tcgagttgac gccagcgcaa gtgacaaaat gtcccttcaa gcgtgatgtc tccggataat
     1621 attctgttcg gaatgcccaa tcatagttaa acaccactcc attcatccag ccttgaccag
     1681 ggggataatt attgaaacca gcaaagtaaa accattcgta atggttgaga tccggagtgg
     1741 gtacttctcg cacatcaaaa taataggtgc taccaatgtt caggagaata gttgagggaa
     1801 aatggaattg aacccaggta ggtgctgcga gtgcgccagg gttcataata gatgtaccaa
     1861 ctagttgccc ggcagcgtta tacacgttga tttgtacatc acagggtggt ccattgctga
     1921 caagagtaat attgattgca tcaagtttat tgacactggg gacgaaactt tggtaatccc
     1981 accaattctg cggttggaga ttatcatttg tcgagaacag catctgcatt tgatccacaa
     2041 gtttttccga ctcattgtag aaaccaagta acagaattac atcttgacag cgttcgattt
     2101 catcttcgat gaatgaaaag tttggtgcat tgtatttatt cacggtgaaa actggagaga
     2161 gtcctttgtt gatcagccat gtttgtatcg cattcttcat atcagtgata ttcgtagtcc
     2221 cttttgtcgt ggtattcatc tttgcagcga gatcctgtat caacggagga acattcgttt
     2281 tcagatgatc atcagtgaca ccatatttct gcacgaggga aaagttatca acaccatcac
     2341 caggatatcc atttggatca tcaaattttg agtcaaacca ccagaaacaa tttgcaacgg
     2401 ccacgggtcc acaaaacgcc cattttgcga catcatctcc tgcggggttt gtgttcaacg
     2461 cacaatttgg tccaggtgcg acgaccacag cccctgggct aactgcgcca cccacgggca
     2521 caacttggat atcatctcct aaagcagcag tgtttgcaac cccatttcca ccatctacaa
     2581 tactcttcca ctgatcctgc gtttgattaa aatcaggcat cccctgtgga gcatagttcg
     2641 cgtacgcagg tttatagtac caccctcctt cggtagactg attgaccagg aggagataat
     2701 cctctgtttc tccatcttca aatgagccct caccgatcca attataagga acagctgctt
     2761 cggtgatgga gaatcgagtc cagacatacc cagaatttgg tccgatgaca aaatttggtg
     2821 ggataagtgc agaaagagga ccaatgtaaa gtggtgggac gacgaagttc actagcacat
     2881 gttcccctgc atcgccccag gcgccatttt ggttccagtc cattaacaca ttaacaaagg
     2941 cggggagata cggctcgtga tttggcatgg tattatgtac ccatatatcc acattcacgc
     3001 cccatactgc attctgacct gggctgccaa gagcggaacc agcccatcct gggaagggaa
     3061 cgacattcat tgcgacattg atagtaaatg gttgtggaat gatcaaccct gcatcaccat
     3121 cttgatatcc ctcatcctga tcgtaaggtg gaaaaccacc tgggcaccat cctgcattgc
     3181 cttcccattc aaaatcaaat gtcggaccaa accatgctcc aaagttgttg tgttggatcc
     3241 atgatgctgg accacacccc atgcatgtag gaaacgcacc catgacaccg gtcgccgggt
     3301 atgctatatg cgttggacct tcaggagcat cgccgtactc acgataatct cttccaagcg
     3361 gttcattggc atcattttgt ttctgctctg gtgcaaagtt ccatgcaaca accacagcgg
     3421 tgcttgaggt gagcaacagc ataaaaacta cgattccaat aattttattt ctcataaact
     3481 atcctccgtt acaattgcta tctcacatca tatataaaaa tccattcgct tctaattgaa
     3541 acaaatgtta agaatagtga tttgtgaatt gattccaaca attctttcct atgttatgat
     3601 agtaaaagaa tagtattcct gagactattt tttatcctta taatgaaaat atgcattttc
     3661 aatggtccca tgctgtttca tcagattaaa gacggtgtcg tggaagatga cgtttccatt
     3721 cattaaaaaa tagacagagt ctgcaacttg ttcaacaagt tgagtcgaat gagtagcaag
     3781 gactgttgtt ccttttcttt ttttaataaa atcttgtatt gttcttgatg caagcggatc
     3841 caaaccatag accggctcat caaggagaag attttgtggt tggtgcatga ttgcagaaag
     3901 caaggatatt ttttgcttca tccccttcga atacccaccg agttcataat ccaaagaggg
     3961 ggtaatcccg atcatctgag aaagatcagt gatgatacgt tcggcattaa taagtttccg
     4021 taatgaggca atgtaaaaaa gaaattcccg tccagtgata cggtcataga gctcaggaat
     4081 ttccggaagg tatccgagat gttgtcgtgc gagaagcgac tgtgtcacaa tcgaatacga
     4141 atcaatcatc acaacgccag agtcaaaggg cagaatccct gcgatgatct tcagaagcgt
     4201 actttttcca gcaccgtttt cccccatgat agcgatgacg ttgacatcat tgaactgtaa
     4261 cgatacgtta ttgagtgcag taattttccc atatcgtttg gtgaggttcc gaactgaaat
     4321 cattgtacga gttgtgagaa agaaaaaggg gttaataaga catctgatgc gtgtatgtca
     4381 ttacgacttt gtcctcttcg aatatgactt tttcatattt acgataatga gaccaccaat
     4441 gacaagtgct cctccgaaaa taaacagcca ggtgataagt tcctgaaaaa gaaaataact
     4501 gatgatcgtc gaaagaaccg ggataaaata aagaaatacg cttatctccg aagctgtttt
     4561 tatctctaaa gcgacatacc acaggatata tccgatcact gttggcaaga gtgctaaaaa
     4621 gagaacagcg ccccatcctg cccatgataa tgctgttgtt tctgttatca acgacgatga
     4681 gagaaatggc agcagaccaa gacttccaaa aagaaacgca taaacggtga gcgaaagcgc
     4741 tgagtatcgt tgtaagagtt ttttcccagc tatagtgtat c
//
LOCUS       DATMXJ010000197        16181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_71352, whole genome shotgun sequence.
ACCESSION   DATMXJ010000197 DATMXJ010000000
VERSION     DATMXJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71352"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..773)
                     /locus_tag="VN377_05175"
     CDS             complement(237..773)
                     /locus_tag="VN377_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWR27810.1"
                     /translation="MKTTPKRIIEQLSQDEVQILTALQKNSKDSVSSIAKYCGFSRQK
                     VKRILTRLENDNVIWGYTAIIDEKKQGLLKYVLLIRRSMDKIDKNTAEKITFQIYDED
                     YEKLGITIESWYLIHGEYDWMIIFTSQNLRDAKKFSTLLIEKYPNIIVKINLMQILHS
                     PKTHHIISPTPESLLDFL"
     gene            957..1448
                     /locus_tag="VN377_05180"
     CDS             957..1448
                     /locus_tag="VN377_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27811.1"
                     /translation="MSEEAHNIGKETEGLGWRVLLSILVGVGWLVFLVLWLFFYAKYY
                     AWEKNVAIFLLSLLLLIWVLGVPWAYWTYKKQTSDEKEMWRQKGFRWRFWVSIGVGFS
                     VILFLIYWFWVFAEPFDIYQNLAIFIVTLLIAGGLVAAIWVPWGMKYGSGLHHHPEGD
                     DKK"
     gene            complement(1482..2195)
                     /locus_tag="VN377_05185"
     CDS             complement(1482..2195)
                     /locus_tag="VN377_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HWR27812.1"
                     /translation="MIDMMIAVTSFLNQFIGYLFVILLPVLVLFFVYLIVTKAFNDMG
                     FSSFEAIIIVFVSFLLGSGIIDEYLGINVSNIPLFQYHTNWIVGINIGGAVIPIILSI
                     YLSLKNRLKPVMVLLGIGIVAVVTFLVTYPDPEKGIVSTFPFWILPIVTASFFSIVVA
                     WKEKNKAAPFAYTIGTLGVLIGADGFHLMSLLQYETQTMRVAVIGGASVFDMVFITGI
                     LAVFLDGLLIIQKKRKKDV"
     gene            2406..6155
                     /locus_tag="VN377_05190"
     CDS             2406..6155
                     /locus_tag="VN377_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HWR27813.1"
                     /translation="MSNKRDSKKSRIEAVVIALLFIGTALLVPINTIATNPQSNPTTH
                     DDSQKNYYTEGSEGPFVLDRSYYLIDPNPASMDATDNDDAGYKKDAGIDIARALALYP
                     GELIDETPGRGRSGKISSTDSSDWYYFSVTQGQQLVFSVTPPTGFDVNISLWTATGAM
                     VTSSNNSGSTPETITYTATYNGKWYLCIKYVSGTGTGKYTFTVGFTNQNDANTGADAP
                     NVRTSAILLTPGTYFGYLDMNDPYDWYKIQATSGQGINVTLKMKDLTYLNDFDLQLYN
                     PSGTLVFEGNQYYDDNFNYPADVTGQWSIRVDIFPGWVDVPTPTNWSYYSYGSGVYNL
                     TLKLETSGVAQAPIPQPDITPIAKTFKIINDPDSTKDDFGYLAAIPACNYLDGGQRYL
                     APIIYTGDATPTAYYDDPTAFGTVDDMTQYLVDDWNDYLALFSKTAEQYTVPADPIQA
                     AADIATQGWASSQTAVVAVDGSSFNDTSRTVVKKTKTLTRTVNVLDVQNDDPKLSSSF
                     GYMMFLGPKWCAIGVNTSAISITSGAAYGSLLTQVFPKFMDVASDDWPVPYDGTGNAG
                     DIYYPVTRVGFWSALSQLATDKFDFIRITKYAGDRYRFKVNSNDAVINAALTTDQPSD
                     LLVFLVDPLGNLRSPIMPAWNGPVNPIHIWNGLENPAVNPWRSWNPAPHTEFSAEVLH
                     PETGTWTVIVVPRNAEGSNVKYTLTTTVRTVNPDRADATISAANAAVIASLNHYPLLY
                     VNKDSVPAATASAFTALGVTKVIFVERGEIGKDVRSKLTPTVEKDLITMQQIVDEIKS
                     YPASENYVTATSLKTGDGHFAPAAMLAAYHGSPVIRVEDAPDGDPAAVAERIQSAQRW
                     GGDYYHGSRANMHLPQAPAEVEQNKFKLMIAFIQYLLKGNASVLPPLGLDAKRYWNEE
                     MFNDFHNYIDSLGLDREGQEGYAIVAPRSDINMELHSVLMGNNSYAGDIPGITPAYSN
                     DIVVRDILYPALIFANPGKDITTSSLMNYPENNAFTLNDGKSHKAYSSSTVKNTFSSH
                     LRTFDGHTFWLPFIERMNDGASVYYYSGHGTGGSGMSEMYEQSDLSNYPDQIWWDSWR
                     GYMYDNWKMPREVISGNVWFNPEPPMLYDLIHYKWVDQSFQNLHSNAVFYMSCTTGDG
                     DAPLVFLEHGALFWYGNANTGLCPEADIGDDAVFEATLINGVSIGVAWSQQVWLHYRD
                     FTTGDPTSMYGRSSLYPVSSIQVIYGDPNLIVYSPEWQTPVPIDA"
     gene            complement(6268..6678)
                     /locus_tag="VN377_05195"
     CDS             complement(6268..6678)
                     /locus_tag="VN377_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWR27814.1"
                     /translation="MKEGVARFWREIPQRYNFIGNTCEICDRVYFPPRESCPYCRRKS
                     IGKMKEVKLSGKGEVITYSIIHVGPSGFEEQTPYPIAIIKLEEGPQVTAQIVDSPLDE
                     IKIGMKVESTFRKIQQDGYTGAIYYGYKFKKLRE"
     gene            complement(6678..7847)
                     /locus_tag="VN377_05200"
     CDS             complement(6678..7847)
                     /locus_tag="VN377_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWR27815.1"
                     /translation="MRDVAVIGVGLTKFGELWDKSFRQLIAEAGSKAILDAKIEGKDI
                     DALYVGSMSSGRFVDQEHIGALVAEVSGFSQLHIPATRVEGACASGSLAIREGYLSIA
                     SGINDVVVVGGIEKMNDVGGSEATDILAAASDQEWEAFFGATFPGIYAMIATRHMHEY
                     GTTKEQLAQVAVKNHANGVNNPYAQYQSPISLESVMKATMVAYPLGLMDCSPVSDGAA
                     SVVLCAAEKAKKYTDKPVKIIGSGQASDTLALHGRRDICTLDATVYAAKMAYKQANIT
                     PKNIDFAEVHDCFTIAEICAIEDLGFIEKGKGGTAIENKITTMDGPLPINTSGGLKAK
                     GHPVGATGVAQIVELTMQLRNEAGKRQVKDAKIGLAHNVGGSGATCVVHIMEAMR"
     gene            complement(7854..8903)
                     /locus_tag="VN377_05205"
     CDS             complement(7854..8903)
                     /locus_tag="VN377_05205"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HWR27816.1"
                     /translation="MEELGIVSYGAHIPRFRIKAEVIAKVWGKDGQAISRGLGIFEKS
                     VPSVDQDTATISVEAIRYALKRCTINPSEIGAVYVGSESHPYAVKPTGTIVGEAIGAT
                     PDLMVADYEFACKAGTAAIQTCYGFVKAGMIKYGVAIGADTSQAAPGDALEYSASAGG
                     AAFIIGKTNVITTINHTLSYTTDTPDFWRRAEMKYPSHGGRFTGEPAYFKHIMNCANN
                     LMTTACTKPQDYTYAIFHQPNGKFPVNVAKKLGFTMDQIKQGLMVPYIGNTYSGASMI
                     GLASVLDVAKPGDRIFMTSYGSGAGSDGFDMTVTDNIKRLARQKAPSVAELVADKEYV
                     DYGVYSKMTNLLFLE"
     gene            complement(8984..9871)
                     /locus_tag="VN377_05210"
     CDS             complement(8984..9871)
                     /locus_tag="VN377_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27817.1"
                     /translation="MIEKESTKKEDLEQLKNAEKKYQSLIEKRNELNQVAKQFREERD
                     MLNEKRKELKNDMDKIKKEREKLVAKMKEHKEKRNQYQQTAKELIDAKRKKKGDVIRN
                     LPLRVEEIKADIQMMEYEQETTILDTRKENKLIDRIKLKRKEYNEAKKQMEKQQLIEV
                     DLTDTDKSITELFKKADTEHEKVQKYYNESQQKHEEYKKLVNEIATLIGEANKKHKKY
                     IEVREEAQSNHEKALEMRSKIVTVKKERMQRFQENREILRSQNLKARKELMDNDKLEK
                     VAEQSVEALKGGKKITLSG"
     gene            complement(9922..12798)
                     /locus_tag="VN377_05215"
     CDS             complement(9922..12798)
                     /locus_tag="VN377_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWR27818.1"
                     /translation="MQAHKALKGLLLIFILILGSLLPVFLIPVARAENNQGYTTLYFT
                     NALNFETEGDATTLTQTPPTSQENSEYPPSILTKNGILGKNNTLISEEWFLWFSSALI
                     GQLLDNLSLEGLELLFPGLNRISEVYIYNGNDTLNIRGDILYHLYFSDTRKIKKLTDS
                     VDVSLYTISSDSPFGLPSLIKNTTVKLTPPKYLGGIYNQQITLPNVNCTLNTGDTLLV
                     SIEIIPTNKSTLALDILNSPIIKKTVQWMVNRWENNITRGPLRRQIGALIKNVTTLLE
                     DTGNESDINFTSEDIASFLNTMKSSKLIYDSQSHPSSVTIPAKISEEDIRIYYLQSGQ
                     ILSETQQEGTNTSKTEKVDLTTTPKIWTANQGLERNKILKVQDVTAELYFYRLLFIIH
                     PKVSVTVTLYDNNTTIASTERQLTKKEVQGFLRKKITPVVFNFTGVDREITYDHKLGI
                     GISLSNGTKTVLTKLKLQLNSIEYRSSLRVKFEETKNIQIHNLTTTPSDGKIIPGGSV
                     QYLFNVTSINPDTLQISTIERAKTGAWSLSATPSVAVSAGSWVTIPVFVNSSNVLKEA
                     YGSTIDLIVVITGNTGVARQTIAAEISEDAIKYKVEILGYSSGINISKGENRFFYFIV
                     KNNNTGAIDDVDSYTITASSQNHWPLIPQETIRNLGIGDTTNAEDAKVFIRVPENTTE
                     TSDVITITVTSDSNSEAYATITITVKVNGGDFIEQILNFFDSAAQSLGLNDIFGTDGK
                     FVLLILLVVIILFFLIILAIVLTSKPVRIICTDRIKEIESTQKAIFEVTLKNPYKKTQ
                     SYEIDARQPTPSSKWEITLDPTTTTIEGRASKTIQIIVTPTDTAEPKEWTQVTISVKK
                     TGKKKKESIDLVTMMKEGKTLLHLQNVSHWPTTFNPGEKVITSFNLTNNGTIAARNVK
                     VFFYLNGKQKNKVEVTLAAGNIADIQIPWIAEKGKNQVHIRVKE"
     gene            complement(12958..14475)
                     /locus_tag="VN377_05220"
     CDS             complement(12958..14475)
                     /locus_tag="VN377_05220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWR27819.1"
                     /translation="MDTDKIIKELSQNEKKVLLALKKLHGRGSPEDIFKTGGFTQDVE
                     ISNAASWLQSKKLVTVDNHIKTVYSLGKEGKRFVEKGLPEKRALKLLAEKLGTVGLKD
                     LSAVLESDEIPVAIGWLKTNGWATLRKEKETTLEITPKGKNALSEVTEEEKLLRLLYE
                     HPEQEIEKNTIKPLASRKNVIKEKDLISSSVTLTDSGKKIVDAGFEIREEISQVTSAI
                     IKNDEWKQKTIRPYDIHAFAPALYGGKAHPLVKLISEIRQIFVEMGFQEIQGDYVESC
                     FWNMDVLFIPQDHPAREMQDTFYCKVPSRIPINDPQLVKEVARVHENGGTTGSTGWGY
                     TFSKTEGERALLRTHTTVNTIRYLYNHPTPPCKVFSLERVFRNEDIDTTHLPEFYQIE
                     GIIYEENANFRQLIGILKEFYRRMGFEKIRFRPGYFPYTEPSMEVEVFWNGKWMELGG
                     SGIFRPEVTEPVGVKTPVLAWGLGLERLAMLRYGLTDIRSLYISDLDWLRKTPLL"
     gene            14585..14989
                     /locus_tag="VN377_05225"
     CDS             14585..14989
                     /locus_tag="VN377_05225"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWR27820.1"
                     /translation="MIGKLSEDIIQAVLETIPFEFSVIDRNEKVLAWNKHSTRIFKRP
                     EAVVGRDVRNCHPKKSLGKVEQILQEMKAGKRDKARFYIDLPLGPNQEKQKVMIEYYA
                     LRDAQGKYLGCLEASQNIAELQKLSGEKRLLD"
     gene            complement(14986..15255)
                     /locus_tag="VN377_05230"
     CDS             complement(14986..15255)
                     /locus_tag="VN377_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27821.1"
                     /translation="MKKHKYPEANRKARFKRRNLGYLPLFDNPFPRYDVRSVDMHHVN
                     RMICIPLPHRTHMIVKGRNCEKHKQRCQEWIQKLFLLDLETLLHP"
     gene            15435..15509
                     /locus_tag="VN377_05235"
     tRNA            15435..15509
                     /locus_tag="VN377_05235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15470..15472,aa:Ile,seq:gat)
     gene            complement(15491..>16181)
                     /locus_tag="VN377_05240"
     CDS             complement(15491..>16181)
                     /locus_tag="VN377_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWR27822.1"
                     /translation="YSNNKYENILLQTIVFLGFNFGMRPPSEICNLDLDDIMINKDGT
                     GYIRIHEDKKRGKQRIIIPFNKNILSSTVFKTPKNYIDNWRKKVATKDSGNALFLQPN
                     GTRITGKYLRDHITKTGKRIAGPYFHLYTMRHTCATYLYEYTKEIEIVRRRLGHRKAS
                     TTIIYVHIADSIKRQVGKKRNLFNLALRSRHQGVRGQQKYIDMDSLDCWAKKHQSNNF
                     SPRNKDGPGEI"
BASE COUNT         4029 a         3447 c         3578 g         5127 t
ORIGIN      
        1 tccctacttt tctttaaccg attcgctttt tcttgaaatt cgtctttttt tcggaccatg
       61 tcttctaacg gttcgtcgtc cattttctgc ctcttactca acggaaaaac acggaggtaa
      121 aatactgggt ctttattaaa agttttctga taattatcgt atttgcctcc tgtccttctt
      181 tttactctga agcagaaatg tttttataag attatcggtc aatcattagc atggtattat
      241 aaaaaatcta atagcgactc aggcgtcggg cttatgatat gatgagtttt cggggaatgg
      301 agtatttgca tgaggttaat cttcacgata atatttggat acttttcaat gagcaaggtg
      361 ctgaattttt ttgcatctct tagattttgt gaagtaaaga taatcatcca atcatattca
      421 ccatgaataa gataccaact ttcaatggtt attcccagtt tttcgtaatc ttcgtcatat
      481 atttgaaatg tgattttttc cgctgtattt ttgtcaattt tgtccattga tcgtcttatt
      541 aacaatacat atttgagaag tccttgtttt ttctcatcga taatcgcggt gtacccccag
      601 atcacattgt cattttccaa tcgtgtcaat attcttttca ctttttgtct tgagaaccca
      661 cagtattttg cgattgaact gacactatct ttggagtttt tttgaagcgc agtcagtatc
      721 tgtacctcat cttgactgag ttgttctata attctttttg gagtcgtttt catatgaatt
      781 tatggtaccg caacttcgac ttctataaaa aagtaactct tatgaagacg cggctgaaat
      841 cgttggtcat gtaaaaaaac attatttttc atctatcaat ttttccgggt tatagtgttt
      901 atatagggga atcggatttc tctccatatc tccagaatga ttcaggggta ttttctatga
      961 gtgaggaagc gcataatata ggaaaagaaa cagaaggtct tggttggaga gtattgttat
     1021 ctatccttgt tggcgttgga tggttagtat ttctcgtgtt atggttgttc ttctatgcaa
     1081 aatattacgc atgggagaaa aatgtcgcga ttttcctcct gtcgctgttg cttttaatat
     1141 gggtcctggg ggtaccctgg gcgtattgga cttacaaaaa acagacctct gatgaaaaag
     1201 aaatgtggag acagaaagga ttccgatggc gattttgggt ctcgattggt gtcgggttct
     1261 ctgtcattct ctttttgatc tattggttct gggtcttcgc tgaaccgttt gatatctatc
     1321 aaaaccttgc gattttcatc gtgaccttgt tgatcgcggg gggtcttgtg gcggcgatat
     1381 gggtgccctg gggcatgaaa tacggctctg gacttcacca tcatcctgag ggagatgaca
     1441 aaaagtaacg catcgtgaca cagcagtact atcgaaatat tttacacatc tttttttctc
     1501 ttcttctgaa tgattaacag gccgtcgagg aataccgcta ggatccctgt gataaaaacc
     1561 atatcgaaca cactggcacc accgatcacc gcgactctca tagtttgcgt ctcatactgt
     1621 aataaactca ttagatgaaa accatcagct ccgatcagca cccccagtgt tcctatcgtg
     1681 taggcgaaag gcgcagcctt atttttttct ttccaggcca caacgattga aaaaaaactt
     1741 gcggtgacga tagggagaat ccaaaatgga aacgttgaga caatcccttt ctcaggatca
     1801 ggataggtga ccaaaaaagt cacgaccgct acaatcccga ttccaaggag gaccatcacc
     1861 ggtttcaacc ggtttttcaa agataagtag atactgagta taatggggat cactgcacca
     1921 ccaatattga ttccgacaat ccaattcgta tgatattgaa acaatgggat attggaaaca
     1981 ttgattccta gatattcgtc gatgatccca gatccaagga gaaatgacac aaagacaata
     2041 atgatcgctt caaaggatga aaaacccatg tcgttgaatg cttttgtcac gatgagatac
     2101 acgaaaaaca acaccagaac aggcaacagg ataacaaaaa gatagccaat gaattggttt
     2161 aaaaatgatg tgactgcaat catcatgtca atcattagga tttagataaa aggtttattt
     2221 ataaacttaa ttaaagaaat actgtgttca ttttttgctc ctttcgacga ttgttcttca
     2281 agacaagggt aagaaatgat tctgttagga tgaaaaccac gtttatagtg gatattttta
     2341 ttactataat cgaaacatat atataatggc cattcatata atcattacat tcaaaggggg
     2401 caaatatgag taataaacga gattctaaaa agtcaagaat agaggcagtt gtcatagcgc
     2461 tattatttat cggaaccgcg ttacttgttc caataaatac catcgctacg aacccacaaa
     2521 gcaacccaac aacccatgat gattcacaaa aaaattatta cactgaaggg agtgaaggac
     2581 cttttgttct agatcgaagt tactatctga tagacccaaa ccctgcatcc atggacgcta
     2641 cggataacga tgacgcaggc tataaaaaag atgcaggaat agacattgcg agagccttag
     2701 cattataccc tggagaactt attgatgaaa caccaggtcg tgggagatca gggaaaatct
     2761 cttccactga ttcaagtgat tggtattact tctctgttac tcagggtcaa cagcttgtct
     2821 tctccgtaac accacctaca ggttttgacg tgaatatctc cttatggacc gctaccggcg
     2881 ccatggtgac atcctcaaac aactccggca gtacccctga aaccatcacc tatacggcca
     2941 cctacaatgg aaaatggtac ctttgcataa aatacgtcag tggaacaggt acagggaaat
     3001 atacctttac ggtaggtttc accaatcaga atgacgcaaa cactggagct gatgctccaa
     3061 atgtccgcac ttcagccata ctactcacac cagggacgta ctttggatat ctcgatatga
     3121 acgatccgta tgattggtat aagatccaag ccacgtccgg acaaggaata aatgtgacct
     3181 taaaaatgaa ggatctcacg tatctaaatg attttgacct gcagctctac aaccccagcg
     3241 ggacactcgt cttcgaaggg aaccaatact atgatgacaa tttcaattac cctgctgacg
     3301 tcaccggcca atggagcatt cgtgtcgata tcttccctgg atgggtagat gttcctacgc
     3361 cgacaaactg gagttattat tcctatggtt ctggtgtcta taacctgaca ttaaagcttg
     3421 aaactagcgg agtcgcccaa gcaccgatcc cacaaccgga cattactcct atcgccaaaa
     3481 cgttcaagat tataaatgac ccggacagta ccaaagacga tttcgggtat cttgctgcaa
     3541 tccccgcttg taactacctt gacggcggac aacgctatct cgcaccaatc atctacactg
     3601 gggatgcaac accaacagcg tattacgatg acccgaccgc ctttggaacc gttgatgaca
     3661 tgacacaata ccttgttgat gattggaatg actatcttgc gttatttagc aagaccgcag
     3721 agcagtacac ggtcccggca gatccaatac aagcagccgc ggatatcgca acacaaggat
     3781 gggcatcttc tcagaccgcg gttgtcgcag ttgacggtag ctcctttaac gacacatcaa
     3841 gaaccgtagt aaagaaaaca aaaacactta cccggactgt taatgtcctg gacgttcaaa
     3901 atgatgatcc aaaactttct agcagttttg gatacatgat gttcctcggg ccgaaatggt
     3961 gcgcaattgg tgtgaatact tcagcaatta gcatcaccag tggagcagcc tatggttcct
     4021 tgcttaccca agtgttccca aaatttatgg atgttgcctc tgatgactgg ccagtacctt
     4081 atgatgggac tgggaatgca ggtgatatct actatcccgt aacacgagtc ggattctggt
     4141 cagcactctc acaacttgca accgataaat ttgattttat tcgcataaca aaatatgctg
     4201 gcgaccgata ccggttcaaa gtgaattcaa atgatgctgt aatcaatgcg gcactcacca
     4261 ccgatcaacc atctgatctg cttgtatttc tcgttgaccc actgggcaac ctgcgatcac
     4321 caattatgcc tgcgtggaac ggaccggtaa acccaatcca tatttggaat ggacttgaaa
     4381 acccagccgt gaacccatgg agaagctgga atcctgcacc ccatacggaa ttttccgcag
     4441 aagtccttca tcctgagacg ggaacatgga ctgtcattgt tgttcctcgg aatgcagaag
     4501 gatcaaacgt gaaatatacc cttaccacaa cagtgagaac tgtaaaccct gatcgagctg
     4561 atgcaacaat ctcagctgcg aacgccgcag ttatcgcgtc gttgaaccac tatccattgc
     4621 tttatgtcaa caaagatagc gttcctgcgg caactgcaag tgcatttacc gctcttggtg
     4681 tgacaaaagt tatttttgtt gaacgtggtg aaatcggtaa agacgtacgc agtaaactga
     4741 ctccaactgt tgagaaggat ttaatcacca tgcagcaaat cgtggatgag attaaaagct
     4801 atcctgcctc tgagaactac gtcacagcaa cgtctttaaa gactggcgat gggcactttg
     4861 cccctgcagc tatgcttgca gcctatcatg gttctcctgt catccgggtt gaagatgccc
     4921 ctgatggaga ccctgcggca gtagcagaac ggatccagag tgcgcaacgc tggggaggag
     4981 attactacca tggatctcgt gcaaatatgc atttaccaca agctccagcg gaagttgaac
     5041 aaaataaatt caaactgatg atagccttta tacaatattt actgaaaggt aatgcaagtg
     5101 tacttccacc gctaggtctt gacgcaaaac gatactggaa cgaagaaatg ttcaacgatt
     5161 tccataacta tatcgacagc ctcggacttg atcgagaagg ccaggaggga tacgccattg
     5221 ttgctccacg ctctgatatc aatatggagt tacattctgt gctaatggga aacaactcct
     5281 acgcgggtga catcccggga attacccctg cctattcgaa tgatattgtc gttcgagata
     5341 ttctgtaccc ggcattgatc tttgcgaacc ccggtaaaga tatcacaaca tcctcgctga
     5401 tgaactaccc tgaaaataac gcttttacgt taaatgacgg gaaaagccat aaagcgtact
     5461 cctcgagtac agtgaagaac actttcagtt ctcaccttcg aacctttgat ggacatacgt
     5521 tctggttacc attcatcgaa cgtatgaacg atggtgcaag cgtgtattat tactccggcc
     5581 atggaactgg tggaagtggc atgtcagaaa tgtatgaaca atcagaccta tcgaactatc
     5641 ctgatcagat ttggtgggat tcctggcgtg gatacatgta tgataactgg aagatgcctc
     5701 gagaggtgat ttccgggaat gtctggttca accctgagcc cccaatgctc tacgacctta
     5761 ttcattataa atgggttgat caatcgttcc agaacctcca tagtaacgcg gtattctaca
     5821 tgtcatgcac aacaggagat ggagacgccc ccttggtctt tttagagcat ggtgcgctct
     5881 tttggtacgg gaatgcgaac accggattat gcccagaggc ggacatagga gatgatgcag
     5941 tcttcgaagc taccctcatc aatggagtat cgataggtgt tgcatggtca caacaggtct
     6001 ggctccacta tcgagacttc acaacgggtg acccaacctc tatgtatggt cgttcgagtc
     6061 tgtacccggt ttcctccatc caggttatct acggtgaccc gaatctcatc gtttacagcc
     6121 cagaatggca gacaccagta cccatagatg cgtaaacacg catctccctt ttttcttttt
     6181 tttttaattt atcgtctttt gaaaaatttt tttttagtca aaaataccta taaacaagaa
     6241 agaggaaaaa gaggaaaaat actgttgtta ctcacgtagt ttcttaaatt tgtacccgta
     6301 atagatagca ccggtatatc catcttgttg gatcttgcga aacgtgcttt cgactttcat
     6361 cccgattttt atttcatcga gagggctgtc aacaatctgg gctgtcactt gtggtccttc
     6421 ctccagttta atgatagcga tcggatacgg tgtttgttct tcgaaaccac tcggaccaac
     6481 atggatgata ctgtaggtga tgacttctcc ttttccagag agtttcacct ctttcatttt
     6541 cccgatgctt ttccgtcggc aatacggaca ggattcccgg ggcgggaagt acacacggtc
     6601 gcagatttca caagtgtttc ctatgaagtt ataccgctgg gggatttctc tccagaatcg
     6661 tgcaacgcct tctttcatta tcgcatcgcc tccatgatat gtacgacgca cgtggcgcca
     6721 gagccaccga cgttatgagc aagtcctatt ttcgcgtctt ttacttgtcg tttccctgct
     6781 tcgtttcgaa gctgcatggt gagctctacg atttgtgcaa cccctgttgc gcctaccggg
     6841 tggccttttg ctttgaggcc gccgctggta tttattggta atggaccgtc catggtcgtg
     6901 attttattct ctatagcggt tccacctttt cctttttcga taaaaccaag gtcttcaatc
     6961 gcacagatct cagcgatcgt aaaacagtca tggacttcag cgaaatcgat attttttgga
     7021 gtgatgttcg cctgcttata cgccattttg gcggcataaa ccgttgcatc tagcgtgcag
     7081 atatcgcgtc gtccatggag cgcaagggtg tcagaggctt gtcctgatcc gatgattttg
     7141 acgggtttgt ctgtgtattt ttttgctttt tccgcagcgc aaagcacaac tgaggcagcg
     7201 ccatcactca ccggtgagca gtccataaga cctaaggggt acgcaaccat ggttgctttc
     7261 atcaccgatt ctagggaaat cggggattga tattgtgcat atgggttgtt gacaccattc
     7321 gcatggtttt tcactgcgac ctgtgcaagc tgctcttttg tcgtaccata ttcgtgcatg
     7381 tgccgggtgg ctatcatcgc atagatccct ggaaaggttg ctccgaaaaa cgcttcccat
     7441 tcctgatctg atgctgccgc aaggatgtct gtcgcttctg aaccaccaac gtcgttcatt
     7501 ttttctatac cccccacgac gaccacatcg ttaataccag atgcaataga gaggtaccct
     7561 tcacgtatgg ctaggcttcc agacgcacat gctccttcga cacgggtcgc tgggatatgg
     7621 agctgagaaa accctgagac ttcagcgaca agagccccga tgtgttcctg gtcaacgaag
     7681 cgaccggaac tcattgatcc gacatagagc gcatcaatat cttttccttc tattttcgca
     7741 tcaaggatcg cttttgagcc ggcttcagcg atgagctgac gaaatgattt gtcccaaagt
     7801 tcaccaaatt tcgttaatcc tactcctatg accgctacat ctctcatatg tcttcactcc
     7861 aagaataata aattcgtcat ttttgaatag actccgtaat ccacatattc tttatcggcg
     7921 accagttctg cgaccgaggg tgctttctgt cgtgcgagtc gtttaatatt atcggttact
     7981 gtcatatcga acccatcgct tcctgcccct gaaccgtaac tggtcataaa tatccggtcc
     8041 cctggttttg cgacatcaag gaccgatgca aggccgatca tcgatgcacc agaataggtg
     8101 ttcccgatgt aagggaccat gagtccttgt ttgatttgat ccattgtgaa gccgagtttc
     8161 tttgcgacgt ttacagggaa ttttccattt ggttgatgga agatagcata cgtgtagtct
     8221 tgtggttttg tgcatgcggt ggtcatcagg ttgtttgcac agttcatgat atgtttaaaa
     8281 tatgctggtt ctcctgtgaa tcgtcctcca tgggacgggt atttcatttc tgcccttcgc
     8341 cagaaatcag gggtgtcagt ggtgtatgat agggtatggt tgatcgtggt tataacattg
     8401 gtttttccta tgatgaatgc cgcgcctccc gctgatgcag aatattcaag ggcatctcct
     8461 ggcgctgctt gtgaggtatc cgctccgatt gcgactccgt atttaatcat ccctgctttg
     8521 acgaatccat agcatgtttg gatggcagct gttcccgctt tacaagcaaa ttcgtaatca
     8581 gcgaccatga ggtctggtgt ggcaccaatt gcttccccta caatggtccc tgttggtttg
     8641 accgcgtacg gatgtgattc acttccgaca tacacagccc cgatctctga ggggttgatc
     8701 gtacaacgtt ttaatgcata ccgtattgct tcaacactga tggttgccgt atcttggtcg
     8761 acggaaggaa ctgatttttc aaaaatccct agtcctctac tgattgcttg tccgtctttc
     8821 ccccaaactt tcgctatgac ttctgctttg atacggaagc gcggaatgtg tgccccgtaa
     8881 ctgacaattc ctagttcttc catggtgaat ccagcaaaag caaaaactaa aaagactact
     8941 taaaagtgcc gattaatatt cgacaattga cgtagtgtat ctattatcct gataacgtga
     9001 tcttctttcc gcctttcagg gcttcgactg actgttctgc gactttctcc agtttgtcat
     9061 tatccattaa ttcttttcgg gctttgaggt tctggctgcg gagtatctcc ctgttttcct
     9121 gaaatcgttg cattcgttct ttttttacag tgacaatctt tgatcgcatc tctaaggctt
     9181 tttcatggtt gctctgcgct tcctctcgga cctctatgta tttcttatgt tttttatttg
     9241 cttccccaat aagagttgcg atttcattga ctaatttttt atattcctca tgtttctgct
     9301 ggctttcatt gtagtatttc tggacttttt catgttcagt gtcagctttt ttgaataatt
     9361 ctgtgatgct tttatcggta tcggtgaggt cgacttctat gagttgctgt ttctccattt
     9421 gtttttttgc ttcattatat tcttttcgtt ttagttttat ccggtcaatg agtttgtttt
     9481 cttttctggt gtctaagatg gtggtttctt gttcgtattc catcatttga atatctgctt
     9541 tgatttcttc gactcggagt gggaggttcc taataacatc tccttttttc tttcgtttcg
     9601 catcgatgag ttcctttgcc gtctgttggt attgatttct tttttctttg tgctctttca
     9661 tctttgcaac gagtttttct cgttcttttt tgattttatc catgtcgttt ttgagttctt
     9721 tgcgtttttc gttgagcatg tctcgttctt cacggaattg ctttgcgacc tgattgagct
     9781 cgttgcgttt ctcaatgaga ctttgatatt tcttctctgc gttcttcagc tgttcaaggt
     9841 cttccttctt tgtagattct ttctcaatca caagagacac cctttttcca ataccgaatt
     9901 attctcccat tataaggttt actactcttt cactcgtatg tgaacctggt ttttaccttt
     9961 ttccgctatc caggggatct ggatatctgc gatatttccg gcagcgagcg tgacttcaac
    10021 cttatttttt tgtttcccat tcaggtagaa aaagactttc acgtttcgtg ctgctatcgt
    10081 cccgttattc gttaaattaa aggatgtgat taccttctct ccagggttga aggtggttgg
    10141 ccagtgggac acgttttgaa ggtgaaggag tgttttgccc tctttcatca tggtgacaag
    10201 atcgatggat tcttttttct ttttccctgt ttttttcacg gagattgtca cttgtgtcca
    10261 ttcctttggc tctgcagtat ctgttggcgt gacgatgatc tggattgttt ttgaggctcg
    10321 tccctcgatt gtagttgtgg ttgggtctag ggtgatttcc catttcgagg atggggttgg
    10381 ctgtcgtgca tcgatttcat aggattgcgt ctttttgtat gggtttttca gtgtgacttc
    10441 gaagatcgct ttctgcgttg attctatttc tttgatccgg tcggtgcaga tgatgcggac
    10501 tggttttgat gttaacacga tagcaagaat gattaggaag aataaaatga ttaccactag
    10561 gagtattaag aggacgaatt taccatcagt accgaaaatg tcgttgagtc ctaatgattg
    10621 tgctgcggaa tcgaagaagt ttaagatctg ttcaatgaaa tcccctccgt tcactttgac
    10681 ggtgatggtg atggtggcat aggcttcgct attgctgtct gaggtgaccg tgatggtgat
    10741 gacatctgag gtttcggttg tgttctccgg gaccctgatg aagactttgg cgtcttcggc
    10801 attcgtggtg tctcctatcc cgaggtttct tatggtctct tgtgggatta gcggccagtg
    10861 gttctgcgaa gaagcggtaa tcgtatagct atcgacatca tcaatggcgc cggtgttatt
    10921 atttttcacg atgaaataga agaaacggtt ttctcctttg ctgatattga tgccacttga
    10981 gtatccgagt atttctacct tatattttat ggcatcttca gatatttcag cggcgattgt
    11041 ttgccgagca acaccagtgt tcccggtgat tacgacgatg agatcgatgg tgctgccgta
    11101 tgcttctttc aggacatttg atgaatttac aaacacgggt atggttaccc agcttccagc
    11161 agagactgct actgatgggg tggctgataa ggaccaagct ccggtttttg ctctttcgat
    11221 ggtgctgatc tgtaatgtat caggatttat gcttgtcaca ttgaagaggt attgaacaga
    11281 tccacccggt atgatttttc catctgaggg tgtggtggtt aggttatgta tctgaatatt
    11341 ttttgtttct tcgaatttca ctcgaagtga tgaacggtac tcaattgagt taagctggag
    11401 tttcaacttt gtcaggaccg tttttgttcc gttgcttagg gaaatgccaa ttcctaactt
    11461 atggtcatag gtgatttctc tgtcgactcc agtaaaatta aatacgacgg gagttatttt
    11521 cttacgtagg aaaccttgta cctccttttt tgtcagttgt ctttcagtgg acgcgatggt
    11581 cgtattatta tcatataagg taacggtgac actgaccttt gggtggatga taaaaagtaa
    11641 tctataaaag taaagctcgg cagtgacatc ttgtaccttg agtattttat ttctttcaag
    11701 tccttgatta gcggtccaaa ttttcggtgt tgttgtgaga tcaacctttt cggtttttga
    11761 cgtatttgtt ccttcttgtt gtgtttcgct tagtatttga ccggattgga ggtagtaaat
    11821 tcgaatatct tcttctgata tttttgcagg gatggtgaca gatgaaggat gagactgcga
    11881 atcgtagatg agctttgatg atttcatcgt gttaaggaat gaagcgatat cttctgaagt
    11941 aaaattaata tctgattcgt tccctgtgtc ttccaagagg gtagtaacat tttttatcaa
    12001 tgctccaatt tgccttcgta acggaccgcg agtaatgttg ttttcccagc ggttcaccat
    12061 ccactggacg gtcttcttga taataggtga atttaaaata tctaatgcaa gcgtagattt
    12121 atttgttggg attatttcta tactaacgag gagagtatcc cccgtattga gcgtacaatt
    12181 aacatttggt agtgttattt gttgattgta gatgcctccc aggtattttg gtggtgtgag
    12241 ttttacagtt gtgtttttta tcagcgaagg gagtccgaaa ggtgagtcgc tgctgattgt
    12301 gtagagtgat acgtctacac tgtccgtaag tttttttatt tttcttgtat cagagaaata
    12361 tagatgataa aggatatcgc cacgtatgtt cagcgtgtcg ttaccgttgt agatatatac
    12421 ttcagagatt cggtttaggc caggaaaaag taattccaat ccttcaaggc tgaggttgtc
    12481 aaggagttgg ccgatcaagg cggaggagaa ccaaaggaac cattcttctg atatgagcgt
    12541 attattttta ccaagaattc cattttttgt tagaatgcta ggtggatatt cggagttttc
    12601 ttgtgatgtt gggggagttt gagtgagagt ggtagcatct ccttcggttt caaaattaag
    12661 tgcattcgtg aaataaaggg ttgtatatcc ttggttattt tctgcgcgag cgacagggat
    12721 tagaaagaca ggaagtagtg accctagtat tagaatgaat attagtaata gtcctttgag
    12781 cgctttgtgc gcttgcatat cttctcaacc tcgatagtaa ctatctacct tattattata
    12841 taaatctttg tttacaacac ttaccatctt tgtttaatta taataaggaa tatatttaat
    12901 aacattatgg atgtctcttt ctctattaaa atacacaaaa aagttatgaa aaggacatca
    12961 gagtaatggc gtttttcgta accaatcaag gtcactgatg tataagctcc ggatgtcggt
    13021 cagaccataa cgaagcatgg caagtcgctc cagccccagt ccccacgcga gaaccggtgt
    13081 ttttacacca actggctctg tcacctctgg tctgaagatg ccgcttcctc ctagctccat
    13141 ccatttccca ttccaaaaca cctcgacttc catgcttggc tccgtgtacg ggaaatatcc
    13201 tggtctgaac ctgatttttt caaaccccat tctccgataa aattccttca ggataccgat
    13261 gagctgtcga aaattcgcat tctcttcata gataatcccc tctatttggt agaattctgg
    13321 gagatgggtc gtatcgatgt cttcgtttcg aaacacccgt tcgagggaga agaccttaca
    13381 aggtggtgta ggatggttgt aaaggtatcg aatggtgttg accgtggtat gggtccgtaa
    13441 cagcgctcgt tctccctctg ttttcgagaa tgtgtacccc cagccggtgg atccggtcgt
    13501 gcccccgttt tcgtgaacac gggcaacctc ctttaccaat tgtgggtcat taatcgggat
    13561 tcttgagggt accttacagt agaaggtatc ctgcatctca cgtgctgggt gatcctgcgg
    13621 gataaacaga acatccatgt tccagaagca cgattcgacg taatcccctt gtatctcctg
    13681 aaaacccatt tcaacaaaaa tctgtctgat ttctgaaatc aacttgacga gtggatgagc
    13741 cttcccacca tacagcgctg gagcaaatgc atgaatgtcg tatggtctga ttgtcttttg
    13801 tttccattca tcgtttttga tgatagcgct ggtgacctga gagatctcct ctcttatctc
    13861 aaaccctgca tccacaatct tttttccaga gtcggtgaga gttactgagc tgctgatgag
    13921 gtctttttct ttaatcacgt tctttcgtga tgcaagcggt ttaatcgtgt ttttttcgat
    13981 ttcttgttcc ggatgttcat acaagagacg taacagtttt tcctcctcag ttacctctga
    14041 gagagcattt tttccttttg gtgtgatttc aagagtcgtt tctttttctt tccggagagt
    14101 cgcccagccg ttggttttca accatccgat cgccacaggg atttcatcag attcaaggac
    14161 tgctgaaagg tcttttaatc ctacggttcc tagcttttct gcaagcaact tgagtgctcg
    14221 tttctcagga agaccttttt cgacaaaacg tttcccttcc ttgccaagtg aatagacggt
    14281 ttttatatgg ttatctactg ttacgagttt tttcgactgt aaccaagagg cggcattgct
    14341 tatctcaaca tcttgggtaa acccccctgt tttgaagatg tcctctggcg agcctcgccc
    14401 atggagtttc ttcagtgcga gtaagacctt tttttcgttt tgagaaagtt ccttgatgat
    14461 tttatccgta tccattgaaa cctgaaatta tcttgctacg tattaagatg tcgatggtac
    14521 agtctcattt agaaaaaaat ataaaacact agctcttcac cgtttttaca ttggagcgaa
    14581 tcctatgatt ggaaaattat cagaagacat catacaggct gttttagaga cgattccatt
    14641 cgaattttca gtcattgata gaaacgaaaa ggttcttgct tggaataaac actccacacg
    14701 aattttcaaa cgacctgagg cggtcgtcgg acgagatgta cggaactgcc atccgaagaa
    14761 aagtcttgga aaagtcgaac aaatcctcca agagatgaaa gcaggaaaac gcgataaggc
    14821 cagattctac attgatcttc ccctcggacc aaatcaggag aaacaaaaag tcatgatcga
    14881 atattatgct ttgcgggatg cgcagggaaa gtaccttggt tgcctggaag catcacagaa
    14941 tatcgctgag ctgcagaaat tatctgggga aaaacgtttg ttggattaag ggtgcaagag
    15001 ggtttcaagg tcgagtaaaa acagtttctg aatccattct tgacagcgtt gtttatgttt
    15061 ctcgcaattc cgccctttca cgatcatatg ggtccgatgc gggagcggaa tgcagatcat
    15121 tctattgaca tggtgcatat ccacgctcct gacatcatat ctcgggaacg ggttatcaaa
    15181 caaggggaga tacccaaggt ttctcctttt gaatcgtgct tttcgatttg cttctggata
    15241 cttgtgtttt ttcatggtat tttttctcgt ttacatcata taatttagat tgtagaaaga
    15301 gcgaaagcat gggcgatcga gcatccattc acctcgttag attagagata ctaaagtgta
    15361 tatataagta taatgaacga gattgtgagt attacgtttt tatagaacga gcgtattacc
    15421 ccttttcaac accggggccc gtagcttaga tcggttggag caaccggctg ataaccggta
    15481 ggtcgagagt tcaaatctct ccgggcccat ctttgtttct cggagagaag ttgttagatt
    15541 gatgcttttt tgcccaacaa tctaaactat ccatgtcaat atacttttgc tgtcctctca
    15601 caccctggtg gcgtgatctg agggcaaggt tgaacaggtt tcgttttttc ccgacctgcc
    15661 tcttgatgct gtctgcgatg tggacgtaga tgatggtcgt ggatgctttc cggtgtccga
    15721 gtcgtcttcg gacgatttcg atttctttgg tgtattcgta gagataggtg gcgcaggtgt
    15781 gtcgcatggt gtacagatgg aaatatggtc ccgcgatcct ctttcctgtt ttcgtgatgt
    15841 ggtctctgag gtattttccc gtgatgcgtg ttccatttgg ttggaggaac aaggcgttcc
    15901 cggagtcttt ggttgcgact ttttttctcc agttgtcgat gtagttcttg ggtgtcttga
    15961 agaccgtgga tgagaggatg tttttgttga aggggatgat gattcgttgt ttccctcgtt
    16021 ttttatcctc gtgtatccgt atgtatccgg tgccgtcttt gttgatcatg atgtcgtcga
    16081 ggtcaaggtt gcagatctcg cttggtggtc gcatgccgaa gttgaagccg aggaagacga
    16141 tggtttggag caggatgttt tcgtatttgt tgttgctgta g
//
LOCUS       DATMXJ010000198         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_67005, whole genome shotgun sequence.
ACCESSION   DATMXJ010000198 DATMXJ010000000
VERSION     DATMXJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67005"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1856)
                     /locus_tag="VN377_05245"
     CDS             complement(78..1856)
                     /locus_tag="VN377_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWR27823.1"
                     /translation="MKNKKFVVGGILCLLLVTLLITGCVTQKHVMVPMRDNVQLATDV
                     YVKLQNQQHGAILIRTPYNKDALVLLGMNWAQNDWPTVVQDMRGRFASEGVDTVFRSD
                     HTDGPDTLAWIANQTWSNGKVATFGGSALGITQYLTAGANPSQLACQYIQVATPDMYK
                     HAMYPGGEFRKNLVEEWLKAQGNTDILPELWAHENYTMEYWTNVSLEDKWDQVNVPAI
                     HIGGWYDIFTQGTIDGFLGYQHQGGLGAQGKSKLIIGPWTHGGAGTTTQGELTYPTNS
                     LDNFSQALFWQMVNEYTMDGPTDYDTWPAVTYYVMGDVTDSNASGNEWRYANDWPLPS
                     IERTWYLQPDGSLQTIIPTDVQQFSYLYDPTDPVPTIGGGNLNIPAGPFDQRPVENRS
                     DVLIFTSPVLAQPYEATGAIKARLFVSSDCPDSDFTVKLTDVYPDGHSMLITDGILRM
                     RNRNGFDHWELMEPGSVYQVDVDLWSTSYVWNTGHQIRIAISSSNYPRCLNNPNTADG
                     IYKNTTNAVAQNTVYVDSIRSSCLLLPEIPLNATGHQQSWVPSVQNTLYGVGHPLLQP
                     PERLQNILRRSPFFKQLFEYRPFMIK"
     gene            2030..2176
                     /locus_tag="VN377_05250"
     CDS             2030..2176
                     /locus_tag="VN377_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27824.1"
                     /translation="MKQYHYKEYGGNGEYKQGYRKNYKKKMAPYYNKTTLPWKNSQKS
                     IHST"
     gene            2302..3078
                     /locus_tag="VN377_05255"
     CDS             2302..3078
                     /locus_tag="VN377_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWR27825.1"
                     /translation="MHKTKTELLEIIADIKPKKEFEKEINRQYTLYDELLDKDTIAYL
                     LVDQLGRNTQSITTIAELAPNGDYTVVGRVLSISDSKTFKRKNGSPGKVINLEITDET
                     GTCQLVLWNGDIDSIKNKEIKQGTLIKIINGYTKTGYTGGMEIHLGRWGLLEVEPTDA
                     SSQSQQRQYLCDEISGVLLLKEPTKAFFKDDGEFGFVTTITMKEHNIKKELILWDHNV
                     KDIQTCHIGDSLTLKNVTKKWCNGKTEFHVMQNGSVERRK"
     gene            3149..3304
                     /locus_tag="VN377_05260"
     CDS             3149..3304
                     /locus_tag="VN377_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27826.1"
                     /translation="MRPHYQPPRDRQYFHTKPKIDKRDMAALKKKALLIQRKKNIIRS
                     EERFIRG"
     gene            complement(3301..3627)
                     /locus_tag="VN377_05265"
     CDS             complement(3301..3627)
                     /locus_tag="VN377_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27827.1"
                     /translation="MPDREVPLINAKKTIEKDVILDPQGFFVIEVDTKAIHVEYYSNV
                     YKNKRIVSGILQKVFIGIKADALSDTIAFHIPNLRPEHYLYLGRELQKAQVAYEQKKK
                     YIQGGC"
     gene            complement(3617..4516)
                     /locus_tag="VN377_05270"
     CDS             complement(3617..4516)
                     /locus_tag="VN377_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27828.1"
                     /translation="MKQKIKTSVIGSYPIPINTQDMMKDYFAEQQTSWTQYIEQAVHD
                     MTAAGINFVSDGQTRDPFIQLFTRKLKGCRIRERTEIIGPVKYQNPITVPDQIYAKRL
                     LPRHVGLIGVLTGPYTLTKSSVDSFYHDEKELAFDFAKALRKEAELLLKHVDFISIDE
                     PVFCNELPEYAYELIHTITDGLSRPTRLHVCGDVSRIIPQLVDMPVDILSHEFKASPH
                     LFDEFRENPCTKNMCIGAVRSDDILVESVEEIVNHVKKAITIFDGNVVQIAPDCGQRL
                     LPRDIAYQKLKNLAAAGVVLNAG"
     gene            complement(4513..>5187)
                     /locus_tag="VN377_05275"
     CDS             complement(4513..>5187)
                     /locus_tag="VN377_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27829.1"
                     /translation="VLSFIEKVIPKKQPFSIDIIEPSLKIETLEQLASRKLLPRTIYN
                     SIHIGITKEENEALKTYTPEAAIVVAFNPKDKSPDGKIEVLENGAHLLEAGLLETARQ
                     IGIEKVLIDTAALAPGENSGAAIAALPVIKEEYGLPTGCAIHNVVEKSRWLNDFPFAK
                     KTVDIASNNAIPLFGGDYALFGPIENAELVVPLIAWQDILVSEYTENYFGINPVASHP
                     RRKLLK"
BASE COUNT         1422 a         1112 c         1216 g         1437 t
ORIGIN      
        1 atataatata tatatcttat gaatgaatac tttcttcatg aactcctctg gtataacgag
       61 ggtggtgttt ttcgaaatta tttaatcatg aacgggcggt attcaaagag ttgtttaaag
      121 aagggggagc gtcgaaggat gttctgtaat ctctcaggag gttgcagtag aggatgacct
      181 accccgtaga gtgtattctg aacagaagga acccagctct gttgatgccc agtcgcattg
      241 agagggatct cagggagaag aagacaggag gagcgaatgc tatcgacata gactgtgttc
      301 tgtgcaactg cgttcgtggt gttcttatag atcccatccg ctgtatttgg attgttgaga
      361 catcgagggt aattagagga ggagattgcg attctgatct gatgaccggt gttccagacg
      421 tatgatgtgc tccagaggtc gacatccacc tggtagacgc tgcctggttc catcagttcc
      481 caatggtcaa aaccgtttcg attgcgcatt cgaagtatcc catccgttat caacatcgag
      541 tgtccatcag ggtacacatc ggtgagtttg accgtaaaat ccgaatcagg acaatcagag
      601 gagacaaata accgcgcttt tatcgccccg gtcgcctcat agggttgtgc aagtacaggg
      661 ctggtaaaga tcagtacgtc agatcggttt tccacgggtc tctgatcaaa tgggcccgca
      721 gggatgttga gatttcctcc accaatggta gggactgggt cggttggatc atagagataa
      781 ctgaactgtt gcacatctgt aggaatgata gtttgtaagc tgccgtcggg ctggaggtac
      841 cacgttcgct ctatagatgg gagtggccag tcattagcat accgccattc attcccagaa
      901 gcatttgagt cagtgacatc gcccatcaca taataggtga ccgccggcca ggtgtcataa
      961 tcagttggac catccatggt gtactcattg accatctgcc agaacaatgc ttgggaaaaa
     1021 ttatcaaggg aattcgttgg ataggtcagt tcaccttgtg tggtggttcc tgcgccccca
     1081 tgggtccatg gtccgatgat gagcttggat tttccttgcg cccctagccc gccttgatgt
     1141 tgatatccga ggaacccgtc gatggttccc tgcgtaaaaa tatcgtacca tccaccgatg
     1201 tggatagctg gcacattgac ttgatcccat ttatcctcta atgagacgtt cgtccaatac
     1261 tccatcgtat agttctcatg agcccagagt tcagggagga tgtcggtgtt tccctgggct
     1321 tttaaccact cctcgacaag gttctttctg aattctccac cagggtacat cgcatgtttg
     1381 tacatatcgg gtgtggcgac ttggatgtac tgacaggcga gctgtgaggg atttgctcct
     1441 gcggtcaggt actgggtgat tcctaatgct gatccaccaa aggtcgcaac cttcccattt
     1501 gaccatgttt gatttgctat ccacgcaagg gtatcaggtc catcggtatg atcgcttcta
     1561 aatacggtat cgactccttc cgaagcaaac ctgcctcgca tatcctggac cacggtaggc
     1621 cagtcgttct gcgcccagtt catcccaaga agaaccagtg cgtctttatt gtaaggcgtc
     1681 ctgataagaa tcgcaccatg ctgctggttt tgaagtttta cgtacacatc cgtagcgagc
     1741 tgtacattat cgcgcattgg gaccataacg tgtttttgcg tcacacagcc agtgatcaat
     1801 aatgtaacga gaagaagaca taaaatccct ccaacaacga atttcttatt cttcataaca
     1861 tcatccctat aaaaccatta tatcaaggat attatatata ttgtttcctc tgttgtgacc
     1921 caggttattt tatgcgtttt gacattttga cagagcaaat ataaatatcg ttagcgacag
     1981 aaagcaccat acgttccact ctgagaagag ctggaagaga ggtgaagaaa tgaaacaata
     2041 ccactataag gaatatggag ggaatgggga atacaaacaa gggtatcgta agaattataa
     2101 gaaaaagatg gcaccatatt acaataaaac cacacttcca tggaaaaaca gtcagaaatc
     2161 aatccattca acttaacatc gcaggattac ttatatctta tctttaccgc cttgcaatcc
     2221 caactaccac gccttccttt ttattttttt taatggatgc atagcagact ataagtaaga
     2281 acacggcata ccgtgttacg gatgcataag acaaaaacag agcttcttga aattatcgct
     2341 gacatcaaac caaaaaagga atttgaaaaa gaaattaaca gacagtatac gctctatgac
     2401 gaacttcttg ataaagacac catcgcgtac ttacttgttg atcagctcgg caggaacact
     2461 caaagcatta caactatcgc tgaactcgca cctaacggcg attataccgt tgtcggaaga
     2521 gtgctgagta tctcggattc aaagacattt aaaaggaaaa atggttcccc tggaaaggtt
     2581 attaacctgg agataacgga tgagacagga acctgtcagc ttgtcctttg gaatggggac
     2641 atcgactcaa taaaaaacaa ggagatcaag caagggacct tgattaagat tatcaacggg
     2701 tacaccaaga ccgggtacac aggtggaatg gaaattcatc ttggacgatg gggacttctt
     2761 gaagttgaac caacagatgc ttcttcacag tcgcaacaac gacagtatct ttgtgatgaa
     2821 atctctggtg tgttattact caaagaacca accaaggcgt tcttcaagga cgatggcgag
     2881 ttcggttttg tcacaacgat tacgatgaag gaacacaata taaaaaaaga gcttatcctc
     2941 tgggatcaca atgtcaagga catccagacc tgtcatatcg gggattcgct cactctgaaa
     3001 aatgtcacaa aaaaatggtg caacggcaag acagagttcc atgtcatgca gaacggttct
     3061 gttgagagac gcaaataaac ctcccttgtt cctcgttacc ctaatataaa ccttcttcat
     3121 tatgagatgt caaggaggat gttgtatcat gcggccgcat taccaaccgc ctcgcgatag
     3181 acaatatttt catacgaagc cgaaaataga taaacgagac atggcagctc tcaagaaaaa
     3241 agctttgtta atccagcgga aaaagaacat cattcgctct gaggaacgat tcatcagagg
     3301 ttagcatccg ccttggatat acttcttttt ttgttcatag gcgacttgtg ccttttgcag
     3361 ctcacgtccg aggtacaggt aatgctcggg acgcaggttt ggaatatgaa aggcgatcgt
     3421 atctgacaac gcatctgcct tgattcctat gaaaaccttt tgaaggatac cggagacaat
     3481 ccgtttattc ttgtaaacat ttgaatagta ctcgacatgg attgctttgg tatcgacttc
     3541 gatgacaaaa aaaccctggg gatcaaggat gacgtctttt tctatcgttt ttttcgcatt
     3601 aattaaaggg acttccctat ccggcattca gaaccactcc tgcggcagca aggtttttta
     3661 atttttggta cgcgatgtct cgtgggagca accgttgtcc acaatcaggt gctatctgaa
     3721 caacgtttcc atcaaagatg gtgattgctt tcttgacatg attgactatt tcttctacgg
     3781 attcaacaag aatatcgtca gatcggaccg caccaatgca catgtttttc gtacaggggt
     3841 tttctcgaaa ctcgtcgaaa aggtgaggag atgctttaaa ttcatgggag agaatgtcaa
     3901 caggcatatc gacgagctga ggaatgatac gagacacgtc gccgcaaaca tgcaaacgcg
     3961 ttggacgaga caatccatct gttatcgtgt gaatgagctc ataagcgtac tcgggtagct
     4021 cattgcaaaa gaccggttca tcgatgctga tgaaatcaac gtgttttaag agaagctccg
     4081 cttcttttcg aagtgccttt gcaaaatcaa aggctaattc tttttcatca tggtagaacg
     4141 aatcgacaga ggattttgta agagtatacg ggccagtgag tactccaatg agtccaacat
     4201 gtctggggag gagtcgtttc gcgtagattt gatcgggaac agtgataggg ttttggtatt
     4261 tgaccggccc gatgatctcg gttctttctc tgatcctgca tcctttcagt tttcttgtga
     4321 atagctggat gaaaggatcc ctggtctgtc catctgacac gaagttgatg cctgctgctg
     4381 tcatgtcatg gactgcttgt tcgatgtact gcgtccagga tgtctgctgc tcagcaaaat
     4441 aatctttcat catatcctga gtattaatgg ggatcggata ggatccgatg acgctggttt
     4501 ttattttctg cttcatttga gcagcttcct ccgaggatgc gatgcgacag ggttgatacc
     4561 aaaatagttc tcggtgtatt cagagacaag gatatcttgc catgcgataa ggggaacgac
     4621 cagttctgca ttctctattg gaccgaatag cgcatagtct cccccaaaga gagggatggc
     4681 gttattggaa gcgatgtcaa cggttttttt tgcgaaggga aaatcattta accatcgtga
     4741 tttctcaacg acgttatgaa tcgcacaacc ggttgggaga ccgtattctt cctttatcac
     4801 tggcagggca gcgatagcag cgccgctgtt ctcaccgggg gcaagagcag cggtatcgat
     4861 taggactttc tcaattccta tctgacgagc agtttcaaga aggccggctt caagaagatg
     4921 cgccccgttc tccaacacct cgatttttcc atcgggagat ttatcttttg ggttaaacgc
     4981 gacaacgatt gctgcttcag gggtgtatgt ttttaatgct tcgttctctt cctttgttat
     5041 cccgatatgt atggagttgt agattgtccg ggggagtaat tttctactgg caagctgttc
     5101 caatgtctct attttaagcg agggctcgat gatatcaatg gagaatggtt gtttcttcgg
     5161 aattactttt tcgatgaaag aaagaac
//
LOCUS       DATMXJ010000199        13549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_19845, whole genome shotgun sequence.
ACCESSION   DATMXJ010000199 DATMXJ010000000
VERSION     DATMXJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19845"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VN377_05280"
     CDS             <1..230
                     /locus_tag="VN377_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015492431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HWR27830.1"
                     /translation="KIEADKLHKEFIDHVNNIHELEKNISSTEKKKEKKKKLADVSAA
                     QKEADDIFERFKRGEKLSTEDLMVLQKAGLL"
     gene            258..758
                     /locus_tag="VN377_05285"
     CDS             258..758
                     /locus_tag="VN377_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein bridging factor aMBF1"
                     /protein_id="HWR27831.1"
                     /translation="MPCELCGRECKGGREAIIDGAKMFVCPDCIKYAEGFVQSEPVRT
                     SSPPQPQRLPVKKTKNIERDIYKDKRMEKELVTNWNHLIEHARKKKGLTREELGFKIG
                     ERTVTIAKLENGDLRPSDQTTAKLEKELGIKLMEEVKEVKEVPTGLQKGTKAGFTLGD
                     FIKSEK"
     gene            764..1360
                     /locus_tag="VN377_05290"
     CDS             764..1360
                     /locus_tag="VN377_05290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWR27832.1"
                     /translation="MKKNDFSAAVQALKEGELIIYPTDTLYALGADIYNDTAVKKVFD
                     IKRRPYSIPLPVAVSSIQAIDAIAWMNDTARKVGEKFLPGSVTIILKKKPIVPSTVTS
                     GFDTIAIRIPQNPIALKILSEYGPLTVTSANIHEKKTKGTIKEILQQLKTPIPVCLHD
                     GRKEEDASTIVDLSMKKPRVVREGSISGNELLDVISHG"
     gene            1353..2252
                     /gene="map"
                     /locus_tag="VN377_05295"
     CDS             1353..2252
                     /gene="map"
                     /locus_tag="VN377_05295"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HWR27833.1"
                     /translation="MDESTYEKYKRAGKIAADARDYGVSLLKPGARVYDIATMVEKKM
                     TKNGAGLAFPVNIALNTLAAHYSPCHDDPLTLKKGDVVKLDVGAHIDGYIADTAVTLE
                     LETHLYNAMIQASDEALKKVIEVLTTHLPLSEAGKTVQNTITSFGYKPIENLMGHGLG
                     RYELHSGVSIPNVGNLGGKTTLHDGDVVAIEPFATNGAGHVISGQGSNIYLCKDSIKA
                     KFIRDNKAKVLFDKIHTHFRTLPFAQRWCHALFPNDDVAMKKLSLLGVIKHYPQLLEA
                     KGGIVSQKEHTVIVREDGCEVIT"
     gene            2234..3115
                     /locus_tag="VN377_05300"
     CDS             2234..3115
                     /locus_tag="VN377_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWR27834.1"
                     /translation="MRGDNIKSAKNNGKTEVKIIQLEELTWKRLDALDRDKTIVFIPM
                     SPLEEHGPHLPIGTDLLTARDAAREAIRAIKKKKPELTCVLFPIIPLGYSKMASDFPG
                     TVSTQIKTLKNIVFDVCSSLARFGFKYYVLCTFHMDLGHLKGIYNGMNKSMKTHPIKI
                     IEPWGSYFYSKEVEKREPQVGFDTKKEVHACFRETSLMNYQYPYLVDPSYKDLQSIYR
                     DLYSPRVLGKTFKEIGISEGYVGSPARADSDYGRWYFQQIVETYAQAAIDMIEGKKPS
                     ELPTQTQLLMRSMFWLR"
     gene            complement(3121..4158)
                     /locus_tag="VN377_05305"
     CDS             complement(3121..4158)
                     /locus_tag="VN377_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27835.1"
                     /translation="MICRLRTKILSLFLLLCLAFSGLPLQAQMADPGSGSYPLISSEN
                     IIQEGIQRIRTEQNLRASEPLRELETWFNLHPLIDYASLIGSSITVKFIDGSYVVLMD
                     PLVEKQGFLGDTPDPSFHKRYTGSSNKTAVILNPAESMYGHHQCQRIIKTLLNLDYSI
                     EYRANQAVDVRYIKNNLSADIIYMNTHAGYFDVDGDHQADAVVIATGELWTNETEQKY
                     TYEYQNHLIVKGMVGDQGFVAFTPAFIEEYYTQEQLPDSLVFMATCYALYDTSMAQEF
                     LDAGARVYMGWSQNTVFWTNSITSIQAFRLLSYGLTVQQVCRLIRSGGFYNWLFHSKL
                     IYFGDGSYQLP"
     gene            complement(4243..4905)
                     /locus_tag="VN377_05310"
     CDS             complement(4243..4905)
                     /locus_tag="VN377_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27836.1"
                     /translation="MEKKTLIITISAAVLLVIASFSSVIGSQDVQSSQKIGSPLFAVR
                     TQRSIHTDRMQTIQSHYLGKGVNSHLFQQNQPSLNSALDRTIKMLNANPVFFAKFLKT
                     LSSNPRVIALLQENGMTIAEFKTYLNRLKNDPSLFIDEIRNAGLTLSSQQLQTPQPLG
                     LNTTNPFACVITVLVMLPVVVIIAFIVVLFTLRIIQCMNLNEVVNQIMDQILQELYPE
                     LV"
     gene            complement(4996..7527)
                     /locus_tag="VN377_05315"
     CDS             complement(4996..7527)
                     /locus_tag="VN377_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27837.1"
                     /translation="MKRFLWLGILFLSASWLFFIPQFATPDLVAGSLCVVAGVLCIIG
                     GIRSWVKKQVEIRYTILLIPLVVALFVVSFPYNLGLMVLVIGLLTSLFCYKSERIQGV
                     PLGISLAGVLLLLQTMVFPLYVSFISHGHRIDILSPVVSSLANLLGFHSSTDNGLLFV
                     QTLQQTYPLTITWEKLGFFLVLNMFLGALFLFMMFYEKRQMLKNMMIFLVAGALYILL
                     RFVAILVLYMTTTELSVFFDPLVTTFSFVPFSLLLMKLLPFKDHNEMTLQAPVVHLTK
                     SHLVALILMFLLVFSFTGAFLYQDPGSIKTGRVLIDEYHSQWEDTIRPLDTEWYGLLS
                     TYNYYSWAQWLRYHFSVKTNINASFTPDLLSRYDILILKCPTESYSTQEIQSIKDFVE
                     HGGGLYLIGDHTNVFGMNTFLNQVSEEFGIRYKTDATYELGTGDLSIYKSDQFFSHPV
                     MRHVAQFDFMTSCTLEPTSLSASMRMENIIIGDRIISEPGTYSTENFFRESVASPDSE
                     CGYLLQAAAIKYGAGRVVAFTDSTVFSSFCVFTDGYPSFTLGVMEYLNRTNSLAYLNI
                     VLFALSIAFFVSLVFLLRNNKKMTVLWMFLFAGLLAFCIAAPLCSHLTNLAYPQPTAS
                     TDYPSVFFEQQHSSMNISVKPTSSLGENSDNYGTFFVWTQRVGCVPSFAPTLKDATRS
                     GDIMVIVNPTKPFTETDVQLLTTFFEKGGRLLLMDSIRNPQSTANELLGNFGIWMTTA
                     TKDQEVLSNESDNTSQKPIGNITSPYLTITGGTQLFTNDNNEVYATMAEFMNTTTGEK
                     GRLVVVVDSFTFSDTIMGGTFAEPTERQRQIYDTEFFLFQEVLLP"
     gene            complement(7524..10145)
                     /locus_tag="VN377_05320"
     CDS             complement(7524..10145)
                     /locus_tag="VN377_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27838.1"
                     /translation="MKTLDVFCGMMYKIPDSSVFFSAGLFAANIICILLALNVLSSNF
                     GFVTASSQHLITIFLLLGVVLLNLPLRQAVRHLSKENPHLVQLIIGTLLCLFGFLLLI
                     ISKTQLFWICSLPVILSGLDLCLQGMDHRRNELYLLTIASFGYAVVFLLIDTIPTLWH
                     LYQQSSLVITHAIGVLTTTPFLLGPTTSGLNILLVSLVFLISSFLLATRKTRKEIRWF
                     CFCIVASLFIWFFYLLTLNLIAYPSTDALNLHPLFFLFCLIPIFGFLFRSTVKDTASV
                     IIPKKGWLKHSLKNGKVWAVVFFFLSTLLLTVFITGGIPPADQQKVLFYGDHMIGTWD
                     VPEYGKYGKDAVGMFGLWPIYLTTFGYDTEILVENRTQFLEMAQPPAQNITRYVNLTD
                     YTNVREISSITTKAIDNAAIFVVSNLNASFSEQERSIIWDYVDQGGSLLVIGDHTNVG
                     GIQEPLNELLAPVGIQYRFDAALPFDEKFKWLTCTELLHHPITAPLTSLDELQYGIGA
                     SLDLSSSSFPIIIGSSVLSDIGNRSNGDIAYLGDYEYNKGEQLGDVILVAGAYYGEGK
                     VLVCGDTSSFQNPALPFSYQFLQTSFTWLASKQTGTMKTLQIGVSLLLLIGAAIVYFF
                     FKKDTISFTWFPFFLCLSLLLSALLNPLVLTSSSDSNPSGNLVYIDASHGERFSLESF
                     TDDSLNGLLINLERNNLQPLILRDFSEEKILNSDLIIFNAPTAAFTTNEVVFLQSYMQ
                     RGGVVVLATGYEDKDASLPLLSAFGMDIEPTPLGPVPYVEENLTLYQNEPRFVDAWPV
                     SFQTNQTISFYNFTWGDLTFHLVVFIKHGAGGLLLIGDSQYLLDKNLESIYDYWPGNI
                     LFIKYLLDELPITEENR"
     gene            10306..10434
                     /locus_tag="VN377_05325"
     CDS             10306..10434
                     /locus_tag="VN377_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27839.1"
                     /translation="MYENNKQHEVIEFCLKHDLIKKTDQGYIIKKEFFEILDYFNI"
     gene            10668..10994
                     /locus_tag="VN377_05330"
     CDS             10668..10994
                     /locus_tag="VN377_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27840.1"
                     /translation="MKKQENIYVSIQLEKNVTSGELMIEIQFDRNAPNFFTNKNMISW
                     CPTTEELNFINEANGALNKGKHQRQDHTDKPHVQETEPEELIRNADEKEILDRVFEKK
                     KPAFNQ"
     gene            complement(11006..11947)
                     /locus_tag="VN377_05335"
     CDS             complement(11006..11947)
                     /locus_tag="VN377_05335"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HWR27841.1"
                     /translation="MKKQPVRDILFKKNMTTNQLIKELYDSGGFSAKKVADGVDILER
                     MMHEKDCVKFLSFPACIISTGTRGVIKELVRRKLVDVVITTTGTLDHDLARVWKDYYH
                     GSFMADDTKLHQQGINRLGNIFIPLECYGAILEKKLQPILGELYSMQQKWSTKDLIWE
                     FGKRLEKEKNGKDSILYWAWKNKIPMFVPGITDGAFGSQLWMYYQEHRNFTIDLFKDE
                     QDLSDIVFDAKKTGALIIGGGISKHHVIWWNQFCNGLSHVVYLTTAVEYDGSLSGAQT
                     REAISWGKIAEKADNVTIEGDATVLLPLMISALLERL"
     gene            complement(11958..12839)
                     /locus_tag="VN377_05340"
     CDS             complement(11958..12839)
                     /locus_tag="VN377_05340"
                     /inference="COORDINATES: protein motif:HMM:NF001987.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HWR27842.1"
                     /translation="MKPIRNAVVAGQFYPGTEKNLRQQIEECFLEIRGYGKIPAVQKI
                     KGTLRGLVVPHAGFVYSGAIASHAYGRLAEQGFAETFIILGPNHTGIGSGVSVMTQGA
                     WKTPLGSVPLDESLAQSIATGIIDQDASAHSYEHSIEVQLPFLQYLGQSRTFEFVPIC
                     MMMQDYETVQDVGDILAKAIQRSKKKIILIASSDFSHAGFNYRSTPPSGMRVDEYATK
                     QDTLAIQDILALDPKQLIETVEQHNITMCGFGPVATMLHTAKKLGATRAELLKYGTSY
                     EVHPDSSCVGYGAIAVS"
     gene            12912..13103
                     /locus_tag="VN377_05345"
     CDS             12912..13103
                     /locus_tag="VN377_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27843.1"
                     /translation="MNTGVKQNGTSMETVCHIEILKDGRDFVARAHLANGSVKEYRHQ
                     IFEDVLTEMVIDLQEELAE"
     gene            complement(13140..13349)
                     /locus_tag="VN377_05350"
     CDS             complement(13140..13349)
                     /locus_tag="VN377_05350"
                     /inference="COORDINATES: protein motif:HMM:NF021415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HWR27844.1"
                     /translation="MTEKIPQHMHCQICGKAITASDTETMCSEECKQRYQNLSKKRKL
                     WVYIMYALIFVMIAVLFLAPYLGKI"
BASE COUNT         4137 a         2851 c         3185 g         3376 t
ORIGIN      
        1 caaaaatcga ggcagataag ctgcataaag aatttatcga ccatgtcaat aatatccacg
       61 aactggagaa aaacatctca agcaccgaaa agaagaaaga gaaaaagaaa aaattggctg
      121 acgtatcagc tgcccagaaa gaggcggatg atatcttcga gcgtttcaaa cgcggagaga
      181 aactcagcac tgaagatctg atggtcctgc agaaagcagg attgttataa cgaaataccg
      241 cttggaggga cgttgaaatg ccgtgtgaac tttgtgggag agagtgtaaa ggaggtagag
      301 aagcgattat tgatggggca aaaatgttcg tctgcccgga ttgcatcaaa tacgcagaag
      361 gatttgtaca atcagaacca gtacgaacca gcagcccacc tcaaccgcaa cgattaccgg
      421 tgaaaaagac gaagaatatc gaacgggata tctacaaaga caaacgtatg gaaaaagaac
      481 tggtgacaaa ctggaaccat ctcatcgaac atgcacgaaa aaagaaagga ctgactcggg
      541 aagaacttgg atttaagatc ggggaacgaa ccgtgacgat cgccaaacta gaaaacggtg
      601 accttcgacc atctgaccag acaacagcaa agctcgaaaa agaactcgga atcaagctta
      661 tggaagaagt aaaagaagta aaagaagtcc ctacgggatt acagaaagga accaaggccg
      721 gattcaccct tggcgatttt atcaaatcag aaaagtaaga cgcatgaaaa aaaacgattt
      781 ctcagcagcg gtacaagcgt tgaaagaagg agaactcatt atctatccaa cagacaccct
      841 ctacgcgctt ggtgcagata tctacaatga tacagcggtg aaaaaagttt ttgacataaa
      901 aagacggccc tactcaatcc ccttacctgt cgcggtttca tcaatccagg cgattgacgc
      961 tattgcttgg atgaatgaca ccgcacggaa agtaggtgaa aagtttttac caggaagcgt
     1021 aacgattatc ttgaaaaaaa aaccaatagt gccatctact gtaaccagcg gttttgacac
     1081 cattgcaata cgaataccac aaaatccgat agcattaaag atactctcag aatatggtcc
     1141 attgaccgtg acaagcgcaa atatccatga gaaaaaaacc aagggaacaa tcaaagagat
     1201 tcttcagcag ctaaaaacgc ccatcccggt ctgcctgcat gatggtagga aagaagagga
     1261 tgcctcgacg attgtagacc tttctatgaa aaaaccacgg gtggtccgcg aaggttctat
     1321 tagtggaaac gagcttctgg atgtgatatc acatggatga atcaacatac gagaaataca
     1381 aacgagcagg aaaaatcgcg gcagatgccc gcgattacgg ggtcagcctg ctcaaaccag
     1441 gagcacgtgt atacgacatc gccaccatgg ttgaaaagaa aatgacaaaa aatggtgcgg
     1501 gtcttgcatt ccccgtaaac atcgcactca acacccttgc ggctcactat agcccttgcc
     1561 atgatgatcc actcacattg aagaaaggag acgtggtaaa actcgatgtc ggtgcgcaca
     1621 ttgatgggta tatcgctgac accgcagtga cccttgaact tgaaacacac ttgtataatg
     1681 caatgattca ggcatcagat gaagcgttaa aaaaggtcat cgaagtgtta acgacgcatc
     1741 tgccgctttc cgaagcaggg aagacagtac aaaataccat cacgtcgttc ggatacaaac
     1801 caattgaaaa tctgatgggt catgggctcg gcaggtacga acttcattca ggggtctcaa
     1861 taccaaatgt aggaaatctg gggggtaaaa ccacattaca tgacggggat gtggtagcaa
     1921 ttgagccttt cgcgacaaac ggcgcaggtc atgtgatatc cggacaaggt agcaatattt
     1981 atctctgtaa agattctata aaagcaaaat ttatccggga caataaggca aaagtcctct
     2041 ttgataaaat acatacacat ttcaggacac ttccgtttgc acagcgatgg tgccatgcgt
     2101 tgttccccaa cgatgatgtc gcgatgaaaa aactatcgct cttaggtgtc ataaagcact
     2161 acccgcagct ccttgaggca aaaggcggga tagtctccca gaaagaacat acggtgattg
     2221 tgagagaaga tggatgcgag gtgataacat aaaatcagcc aagaacaatg ggaagaccga
     2281 ggtaaaaatc attcaactcg aggagctgac ttggaagcgg ctcgatgcac tcgaccgtga
     2341 caaaaccatc gtgttcattc caatgagtcc cttggaggaa cacggtccac atctcccaat
     2401 cggtactgat ttgctgacag cccgggacgc ggcaagggaa gcgattcgtg ctatcaagaa
     2461 gaaaaaacct gagctgacct gtgttctttt ccccatcata cccttaggat attccaagat
     2521 ggcatctgat ttcccgggta ctgtttcgac ccagataaag acattaaaga atattgtctt
     2581 tgatgtgtgt agttcgcttg caagatttgg cttcaagtat tatgttcttt gtacgtttca
     2641 tatggatcta gggcatctca agggtattta caatggtatg aataaatcga tgaaaaccca
     2701 tcctatcaaa atcatcgaac cctggggctc ttacttctac agtaaagaag tggagaaacg
     2761 ggaaccacaa gtcggatttg atacgaaaaa agaagtccat gcgtgtttcc gagaaacttc
     2821 cctgatgaac tatcaatatc catatctggt cgatccgtcg tataaggatc ttcagagtat
     2881 ttatcgggat ttgtattcac cccgggtttt ggggaaaacc ttcaaggaaa ttgggataag
     2941 cgaaggatat gtcggcagcc cagctcgcgc tgacagtgat tatgggcgat ggtattttca
     3001 gcagatcgtt gagacatatg ctcaagcagc cattgatatg atcgagggaa aaaaaccatc
     3061 agagctccca acacagacgc agctgctcat gcggtcaatg ttctggcttc gttagctgtt
     3121 ttatggcaat tgataggatc catctccaaa atatatgagt tttgagtgaa acaaccagtt
     3181 atagaaacca ccagatctga tgagtctgca gacctgttga acggtcaaac cgtatgataa
     3241 gagacggaat gcctgaatcg aggttatact attggtccaa aacacggtgt tctgagacca
     3301 tcccatgtag acacgggctc ctgcatcaag gaactcttgc gccatcgatg tgtcatatag
     3361 cgcataacag gttgccatga acaccagaga atcagggagt tgttcttggg tataatactc
     3421 ttcgatgaac gcaggggtga acgcgacgaa accctggtca cccaccatgc ctttgacgat
     3481 taggtgattc tgatactcat aggtgtattt ctgttctgtt tcattggtcc atagctcccc
     3541 ggtcgctatc acgaccgcat ctgcttgatg atcgccatct acgtcaaaat atcctgcatg
     3601 ggtgttcatg taaatgatat cagccgaaag attgtttttg atatacctga catcgactgc
     3661 ttggtttgca cgatattcaa tcgaatagtc aaggttcaac aaggttttaa taatgcgttg
     3721 gcattgatga tgaccataca tcgattccgc tggatttaag atgactgcgg ttttgtttga
     3781 actgcctgta tatctcttat gaaaagatgg atcgggcgta tctcccagga atccttgttt
     3841 ctcaacaaga gggtccataa gaacgacgta ggaaccatcg atgaacttca ctgtgatcga
     3901 tgaccctata agacttgcat agtcgattaa tggatgtagg ttgaaccatg tttctaactc
     3961 tcgaagaggt tcggatgccc ttagattttg ttctgttcgt atccgctgta tcccttcctg
     4021 gatgatgttc tcagaagata ttaggggata tgatccacta cctgggtcag ccatctgtgc
     4081 ttgaagtggt agaccactaa aggcgaggca aaggagaaga aataacgata gaattttagt
     4141 tctcagtcga caaatcatat taccaacagt acccattcac ggaaaatcta tataaatata
     4201 tcgcagagga aataaaaaga aagaaaaaaa atatttagag gctcagacca actcaggata
     4261 gagctcctgc aatatctgat ccatgatctg gttcacgacc tcattgaggt tcatacattg
     4321 tatgatacgc aaagtgaaca acaccacgat aaaggcgata atcacaacca ccggaagcat
     4381 gacaagtaca gtgatgacac atgcgaatgg gtttgtggtg ttcaaaccaa gcggctgtgg
     4441 tgtttgtaat tgctgtgacg atagcgtcag ccctgcgttg cggatctcat caatgaagag
     4501 cgatggatcg tttttcaggc gattcaaata ggttttaaat tcagcaatgg tcataccatt
     4561 ctcctgaaga agtgcgatga cccgaggatt tgaactgagt gtttttagga attttgcgaa
     4621 aaaaacaggg ttcgcattga gcatttttat cgtacgatcc aatgcgctat tcagtgatgg
     4681 ttgattctgt tggaacagat gagaattcac tcctttcccc aggtaatgag attgaatcgt
     4741 ttgcatacgg tctgtatgaa tcgagcgttg tgttcgaacc gcaaacaagg gtgatccgat
     4801 tttttgtgat gattgcacat cttgtgatcc gatcacggag ctaaacgaag caatcactaa
     4861 gagaacagct gctgatatgg ttattataag agtttttttc tccatatttc tttccccaaa
     4921 aaacttatcg ttgttcctgt atttaattgt tgtcctcgta taatataagg gcgttatgag
     4981 gtaaaaagaa aaaatctatg gcaaaagaac ttcttgaaag aggaagaact cagtgtcata
     5041 aatttggcgt tgtcgctcgg ttggctctgc aaatgttcca cccatgatcg tatctgaaaa
     5101 ggtaaaggaa tccaccacga cgacaagccg tcctttctca ccggtggtgg tattcataaa
     5161 ttcggccatg gttgcataca cttcgttatt gtcgttagtg aacaattgag ttcccccggt
     5221 aatggtcaag tatggagacg tgatattacc aatcggcttc tgacttgtat tgtcactttc
     5281 gttggagaga acctcttgat cttttgtggc agtcgtcatc caaatcccaa aattcccaag
     5341 cagttcatta gcggttgatt gaggattcct gatgctgtcc ataagaagca atcgaccgcc
     5401 cttctcaaaa aaggtcgtca gcagttgaac atcggtctcg gtgaatggtt ttgtcgggtt
     5461 gacaatgacc atgatatcac cactcctcgt cgcatctttg agggtgggtg cgaatgaggg
     5521 gacacagccg actcgttggg tccacacaaa gaacgtacca taattatcag aattttcacc
     5581 aagtgaagaa gtgggtttta cagagatatt cattgaagaa tgctgctgct cgaagaacac
     5641 agagggatag tccgttgaag ccgtaggttg cggatacgcg aggttggtga gatgggagca
     5701 cagtggtgcc gctatacaga atgcgagcag accagcgaag aggaacatcc aaaggaccgt
     5761 cattttttta ttgtttctaa gaaggaagac gaggctgaca aaaaaagcaa tcgaaagggc
     5821 gaaaagaacg atgttgagat acgccaagga attcgttcga ttcagatact ccatgacgcc
     5881 cagagtaaaa gaggggtacc catcggtgaa cacacagaaa ctggaaaaca cggtgctatc
     5941 ggtgaacgcc acaacccgac ctgccccgta tttgatggcg gctgcttgga ggagatagcc
     6001 gcattcggag tcaggggagg cgactgattc tctgaagaaa ttctctgtcg aataggtgcc
     6061 aggttcactg atgatccggt ctccgatgat gatgttttcc atgcgcatgg atgcagaaag
     6121 tgaggtcggc tccagggtac aggaggtcat gaaatcaaat tgtgcgacat gacgcatcac
     6181 aggatgggag aagaactggt ctgatttgta gatggagaga tccccggttc caagctcata
     6241 cgtcgcatca gttttatatc gtatcccaaa ttcttctgag acttgattga gaaaggtgtt
     6301 cataccgaac acgttcgtat gatccccaat caaatacaac ccaccgccat gttcgacaaa
     6361 gtccttgatc gattgaatct cttgcgttga atagctttcc gttgggcatt tcaggatgag
     6421 gatatcgtac ctgctgagaa gatctggtgt gaatgacgca ttgatattgg tcttgacaga
     6481 aaagtgatac cgcagccatt gcgcccagga ataataatta tacgttgaaa gcaacccgta
     6541 ccattcagta tccagaggtc gaattgtatc ttcccattgg ctatgatatt catcgatgag
     6601 aacgcgacca gtttttatcg acccggggtc ttggtaaagg aacgctccgg taaaagaaaa
     6661 gacaagcaga aacataagaa tcagagccac aagatggctc tttgtcaaat gcaccactgg
     6721 tgcttgtaat gtcatttcat tatgatcctt aaatgggagg agtttcatga gcaacaggct
     6781 gaaggggacg aaactgaagg tcgtgacaag aggatcaaag aagaccgaaa gttcggtggt
     6841 ggtcatgtac aaaacaagaa tagcgacaaa acgcaggagg atgtagagag cgcctgccac
     6901 aaggaagatc atcatattct tcagcatctg tcttttttca tagaacatca taaagaggaa
     6961 aagagcccca agaaacatgt tgagtacaag aaagaaacca agtttctccc aggtgatggt
     7021 gagcgggtag gtctgttgga gggtctgcac gaacagaaga ccattgtccg tcgaggaatg
     7081 aaaaccaagg aggtttgcca gggatgaaac cactggtgag aggatgtcaa tgcgatgtcc
     7141 atgggagatg aaggaaacat acagggggaa gaccatcgtt tgaagaagca ggagaacacc
     7201 agcaagtgaa atacccaggg gaactccttg tatgcgttca gatttatagc aaaaaaggga
     7261 cgtgagcagt ccgataacca gaaccataag cccaagattg tagggaaatg acacaacaaa
     7321 gagtgctact actaagggga tgagaagaat cgtatatcga atctccacct gttttttcac
     7381 ccagctgcgt atccccccga tgatgcagag gacaccagcg acgacacaga gcgagcctgc
     7441 gacaagatct ggcgttgcga actgagggat gaagaacaac caactcgcgg agagaaacag
     7501 gatgccaagc cagaggaacc gtttcatcga ttctcctccg ttattggtaa ttcatcaagc
     7561 agatatttta taaaaagaat gtttcctggc cagtagtcgt aaatagattc aaggttcttg
     7621 tctagcagat actgactgtc accaatgaga aggagaccac cagccccgtg ttttatgaag
     7681 acaacgagat ggaatgtaag gtcgccccag gtaaagttat agaaagagat ggtctgattt
     7741 gtttgaaacg acacaggcca agcatcgaca aatcgtggct cgttctgata cagggtgagg
     7801 ttctcttcga cgtaagggac agggcctaac ggtgtaggct ctatatccat accaaaagcg
     7861 ctgagaagtg gcaatgacgc atccttatct tcatatccgg tggctagcac aacgactcca
     7921 ccgcgttgca tgtatgattg aagaaaaacg acttcgtttg tggtaaacgc cgctgtcgga
     7981 gcattaaaaa taatgaggtc actattcaag attttttcct ctgaaaaatc tctgaggatg
     8041 aggggctgga ggttgtttcg ttcgaggtta atgagaagac cgttgagcga gtcatcagtg
     8101 aaagattcaa gggagaaacg ctcgccatgc gacgcatcga tatagacgag gttaccagaa
     8161 ggattactgt cactggaaga agtgagtacc aaagggttca gcaacgcgga aagaagtaag
     8221 gaaaggcata aaaaaaaggg aaaccatgtg aaggatatcg tgtccttctt gaaaaagaaa
     8281 tagacaatgg ccgcaccgat aagcaacagc aaggaaacac cgatctgaag tgttttcata
     8341 gttccggttt gcttacttgc caaccaggtg aaagaggttt gaagaaactg atatgaaaat
     8401 ggcaacgcag ggttttgaaa cgaggaggtg tcaccgcaca caaggacttt accctcgccg
     8461 taataagccc ctgctacaag gatcacatct ccaagttgtt ctcctttgtt gtattcataa
     8521 tcacccagat acgcaatgtc accattcgac cgattcccga tatctgagag aaccgaggaa
     8581 ccaatgataa tcgggaatga ggatgaggac agatccaggg aagcaccgat tccatattga
     8641 agctcatcaa ggctcgtgag cggagcggtg atgggatgat gcaggagttc tgtgcaggtg
     8701 agccatttga atttctcatc aaacggaagc gctgcgtcga accgatactg gatcccgacc
     8761 ggggcgagca gttcatttaa tggttcttgg atgcctccga cgttggtgtg gtccccgatg
     8821 acaagcagcg aaccaccctg gtcaacatag tcccagatga tggatcgttc ctgctctgaa
     8881 aatgaggcat tcaggtttga cacaacaaaa atagcggcgt tgtcgatagc ctttgtcgta
     8941 atactgctta tttccctaac gttcgtgtaa tccgtgagat tcacatacct ggtgatattc
     9001 tgagcgggtg gttgtgccat ctccaggaat tgggtcctgt tttcgacaag aatctctgta
     9061 tcataaccga aagtcgtcag atagataggc cacaagccaa acattcccac agcgtctttt
     9121 ccgtatttcc catattctgg gacatcccat gtgcctatca tatgatcgcc gtaaaacagg
     9181 accttttgtt gatctgctgg tggaatgcct cccgtaataa aaacagttaa tagcaatgta
     9241 gacaggaaaa aaaatacgac agcccagact ttcccattct tcagagaatg tttcaaccag
     9301 ccttttttag gtatgatgac tgatgctgtg tctttgactg tagaacggaa caggaaacca
     9361 aagattggaa tgaggcagaa aaggaagaac aacgggtgga ggttgagcgc atcagttgag
     9421 ggatatgcga tcagattgag cgtgagaagg taaaaaaacc aaatgaataa tgaagcaacg
     9481 atgcagaagc agaaccaacg tatttccttg cgtgtttttc tcgttgcgag aagaaaacta
     9541 ctgatcagaa ataccagaga caccagcagg atgttcagtc cgctagtggt aggcccaagc
     9601 aagaacggtg ttgttgtcag taccccgatc gcatgggtta tcaccagcga gctttgctga
     9661 tatagatgcc agagggtagg gatggtatct ataagcagga acactactgc atatccaaag
     9721 gaggctattg tgaggagata aagttcgttt cttctgtgat ccatcccctg tagacagagg
     9781 tcaagaccag agaggatgac agggagactg cagatccaga acagttgggt tttcgaaatg
     9841 attaggagga ggaatccaaa gagacacagg agagtcccta tgattagttg tacaaggtga
     9901 ggattttctt tcgaaagatg acgcacagcc tgtcggagcg gaagattcag cagaactacc
     9961 ccaagtaaca aaaagatggt gatgagatgc tgactggaag cagtgacgaa tccaaagttg
    10021 cttgaaagca cattgagtgc aagaagtata catatgatat ttgctgcgaa gaggcctgcg
    10081 gagaaaaaca cgcttgaatc gggaatttta tacatcatac cacagaagac atccagtgtt
    10141 ttcatataac tttaatggtt tttcaaggga gattctccaa gatgtcatat catttatgca
    10201 ttatcatttt gttattttgc caagctatct ttatatatgt tcattggcaa catgtaacat
    10261 gatatgagtg gataaaaact cttatcacaa cgaggtgaac gagagatgta tgaaaacaac
    10321 aaacagcatg aggtaatcga gttctgccta aaacatgatt tgatcaaaaa gacagaccaa
    10381 ggctacatca tcaaaaaaga attcttcgaa atcctcgact attttaatat ctaaagatga
    10441 ccccatgaca ttgaatcaac aacaaaaaga actaatccta aaattcctca aatccacagc
    10501 gcaggataat caattagtca aagaagtcgt cacaaaagaa taagaggatg caacacacaa
    10561 ttttaaatat ttttttctaa taaaaaaatc aacggtctca tccttctaaa aagaaaaata
    10621 taaaaccttc tacatgttat cgactgtaca acgaggatgg gaaccgaatg aaaaaacaag
    10681 aaaatatcta tgtctcaatc caacttgaaa aaaacgtcac ctcgggagag ttgatgattg
    10741 agatccagtt tgaccgaaat gcaccgaact tttttaccaa taagaacatg atctcctggt
    10801 gtcccaccac cgaagaactg aatttcatca acgaagcaaa tggagctcta aacaagggaa
    10861 aacatcaacg acaggaccac acggacaaac cacatgttca agaaacagaa ccagaggaac
    10921 ttatccgtaa cgccgatgaa aaggaaatcc tggaccgtgt ctttgagaaa aagaaaccag
    10981 cgtttaacca atgaccatct tggttttata atcgttcgag caaggcacta atcatcaatg
    11041 ggagaagcac cgttgcatca ccttcgatgg tgacgttgtc tgctttttct gctatttttc
    11101 cccaagagat cgcttctcgt gtttgcgcac cggacaggct cccgtcatac tcgacagcgg
    11161 tcgtgagata caccacatgg gacagtccgt tgcagaattg gttccaccag atgacatgat
    11221 gctttgaaat cccgccacca atgatcaatg caccagtctt ttttgcatca aaaacgatgt
    11281 ctgagaggtc ctgttcatct ttgaaaagat caatggtgaa attgcggtgc tcttgataat
    11341 acatccaaag ttgagagcca aacgctccat cggtgatacc agggacaaac attggtatct
    11401 tgtttttcca agcccaatag aggattgaat ctttaccgtt tttttctttt tcaaggcgtt
    11461 tcccaaactc ccagataaga tccttggtgg accatttctg ctgcatcgag taaagttcgc
    11521 ccaggatagg ttgtagtttc ttttcaagga tcgccccata gcattccaat gggatgaaga
    11581 tgttcccaag acggttgatt ccctgctgat gcaactttgt atcatcagcc atgaaagacc
    11641 catggtaata gtctttccag actcgagcga gatcatggtc caaggtccca gtggtggtga
    11701 tgacgacatc gactaatttt cgccgaacca attcctttat gactcctcgt gttcctgttg
    11761 agatgatgca ggcgggaaac gacaaaaact tcacgcaatc cttttcgtgc atcatccgtt
    11821 caaggatatc gacaccatct gctacttttt ttgcggaaaa acccccggag tcgtacagtt
    11881 cttttatcaa ctggtttgtc gtcatatttt ttttaaacaa gatatctcgt acgggttgtt
    11941 tcttcatgag tctgcaccta cgaaaccgcg atcgccccat agccaacaca ggaggaatcc
    12001 gggtgaacct cataggaagt cccatatttt aagagttccg ccctggttgc tccaagtttt
    12061 tttgcggtat gaagcatcgt tgcgaccggt ccaaacccac acatggtgat gttgtgctgc
    12121 tcgactgttt caatcagttg ctttggatct aaggcgagaa tatcctgtat agcgagtgta
    12181 tcctgttttg tggcgtactc atcgacgcgc atccccgatg gtggcgtgct gcggtagttg
    12241 aaccctgcat gagaaaaatc actgcttgca atcaggatga ttttcttctt tgagcgctgg
    12301 atagcctttg cgaggatatc ccctacgtct tgtacggttt cgtaatcctg catcatcata
    12361 cagatcggaa caaactcaaa tgtccgactt tgcccaagat attgcaagaa ggggagctgc
    12421 acctcgatgc tatgctcgta tgaatgtgca gaagcatcct ggtcaatgat acctgtcgcg
    12481 atggattgcg caagagactc atcaagagga actgacccta atggcgtctt ccacgcaccc
    12541 tgggtcatca ctgacacccc tgaacctatc ccggtgtggt tcggtccaag aatgatgaac
    12601 gtctcggcaa acccctgctc tgcaaggcgt ccgtacgcat gggatgcgat cgcccccgaa
    12661 tacacaaatc ccgcatgggg aaccaccaga cctctcaaag taccttttat tttttgtacc
    12721 gccggtattt ttccatatcc tcttatctca agaaaacatt cttcgatttg ttgtctgaga
    12781 tttttttcag tacccgggta gaactggcct gcgacgacag cgtttcgtat cggtttcaca
    12841 accccacgtc ccagccttga catgaggctg aaacacatta accttgcgca caaaacatat
    12901 aaatatcatt gatgaatacg ggagtaaaac agaatgggac gagcatggag acagtctgtc
    12961 atattgaaat cctgaaagac ggtagagatt tcgttgcacg agcgcatctt gcgaacggct
    13021 cggtcaaaga ataccgccat cagatattcg aagatgtcct cacagaaatg gtcatcgatc
    13081 tgcaggaaga actcgcagaa taaacgaaag cattcttctt atttttcgtt acgaccgtac
    13141 tatatttttc ctaagtatgg tgcgaggaat aagaccgcta tcatgacgaa aataagtgcg
    13201 tacatgatat agacccagag tttccgtttc ttggagaggt tttggtaccg ttgtttacat
    13261 tcttcagagc acattgtttc cgtgtctgat gcagtgattg cttttccgca aatctgacaa
    13321 tgcatgtgtt gtgggatttt ttcagtcata ataatcgaac gtacaataga acaggggctg
    13381 aaatagttta ttgtttataa tttcagggcg ggcaactcgt atgtacgcca tgaacgagag
    13441 gatggtagat tcaaaaaaat attttttttc gtataacctg aggggacgac gtccattttt
    13501 ttttatagca acaaaagggt aaagattgga tgattgcact ataaaatat
//
LOCUS       DATMXJ010000200         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_56479, whole genome shotgun sequence.
ACCESSION   DATMXJ010000200 DATMXJ010000000
VERSION     DATMXJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56479"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VN377_05355"
     CDS             <1..1599
                     /locus_tag="VN377_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27845.1"
                     /translation="PINCTLHHLPSTDPAYVYGYRVGNDQGLYNTYFKSNAQNNWPVA
                     FAYNKSTDPTIHVIRSKLMGVGYLDPASDWTYQYLQNVQSSQGQFTGNTITYEDVFTG
                     SDVTWRYRNTELKEEIILSNATKTVLQNHPPSLYSLNNESSYLVFITKLDHQNLNLYN
                     ASGMLTGNVMISDVGVDFKDVFGQFKCALPLGEAYELNNESVRQKLTYRIVHLNGNTY
                     LLSGLKASDLNTMTFPVVIDPTVTLYSTFSDGYIYNYGTNYNTAQSSSYGMESSSATY
                     ITIGQKKVSTMYFINRGFVFFNTSVVPFNALLDNATLALYKKDDNSTTDFDITIQDGQ
                     PTYPHDPLESSDYNKNHYSGNIGALNTSGFTTGYNNITLNNLKCINTNGTTKLCLRSS
                     RDINGTTPTGNEYINVFSSDFGLIGCLPKLIIVYRNQSKIKNTGSTDIQGYILMQVHF
                     YDESQCEWVWDSDVIDEPKPRTINSNSQFGLDMIFNDRIRASDLQHGAGTYRVYTAFR
                     DPEGNILRTNDDLDLEAWWQFNKT"
     gene            2026..2496
                     /locus_tag="VN377_05360"
     CDS             2026..2496
                     /locus_tag="VN377_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR27846.1"
                     /translation="MDNVVISCSSKKIAGDTRNNWVTLMFVTHNRYNCFRKQIYIDIC
                     IQAFHDLERFGFEFGDFGFALNHVHFLVNIPKRYSVQTAEIMLKSYTARKMFETFPGF
                     RKRYPRGGFWSGYDHYESTGWKNLEQSSVYIRDQSRHHNAYVVDDHQQQLPNVA"
     gene            complement(2623..2707)
                     /locus_tag="VN377_05365"
     tRNA            complement(2623..2707)
                     /locus_tag="VN377_05365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2669..2671),aa:Leu,seq:gag)
     gene            complement(2764..3285)
                     /locus_tag="VN377_05370"
     CDS             complement(2764..3285)
                     /locus_tag="VN377_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWR27847.1"
                     /translation="MTVIPNPGQCLRLLREAGCSDEVINHCKAVRKVAVRIAKKAHAN
                     IQLVEAGALLHDIGRSKTQGILHGVEGAKIATQLGLPESIITIIERHLGAGIPKEEAI
                     ILGLPPKDYIPITLEEKIVAHADNLIENDHEHRIEIEVEKALQKGQMKHAERLMALHR
                     ELSQRCGMDLDNI"
     gene            complement(3282..3797)
                     /locus_tag="VN377_05375"
     CDS             complement(3282..3797)
                     /locus_tag="VN377_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604207.1"
                     /note="catalyzes the S-adenosyl-methionine-dependent
                     2'-O-ribose methylation of C56 in tRNA transcripts;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HWR27848.1"
                     /translation="MIAVLRIGHRIERDKRVTTHVALVARAFGADIIYITTKDEKIKK
                     NIQSVCKRFGGNFQVQTGIDHKKIIKSWKGAIVHLTMYGEELEKAIQHLDTNRDLLII
                     VGAEKVPPYFYEIADMNISVGNQPHSEVAALALFLDRFTKAAWQKKLFDGNIQIIPCV
                     KGKKVLSKEKS"
     gene            complement(3878..4255)
                     /locus_tag="VN377_05380"
     CDS             complement(3878..4255)
                     /locus_tag="VN377_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27849.1"
                     /translation="MVKLKLFQRKKTKEETHVEDNVTREEIRCSDEKKIEPETKIEPK
                     EVPIKEYNETLYSKGFAQKQQKTPSFQQKPPIRRTTWENAETIEQNIDNIRPGQSEST
                     TRRTQIGIDTEKKVDFILLKKKK"
     gene            4408..4481
                     /locus_tag="VN377_05385"
     tRNA            4408..4481
                     /locus_tag="VN377_05385"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4442..4444,aa:Val,seq:cac)
BASE COUNT         1637 a         1331 c         1274 g         1949 t
ORIGIN      
        1 ccgattaact gtacgctcca ccacctcccc tccaccgatc ctgcgtatgt atacggatac
       61 cgcgtaggga atgatcaagg gttgtataat acgtatttca aatcgaatgc gcagaataac
      121 tggccggttg catttgcgta caacaaatcg acagacccca cgatacacgt catcagaagt
      181 aaactcatgg gcgtcgggta tctcgaccca gccagtgatt ggacgtacca gtatctgcag
      241 aacgtacaaa gcagccaagg acaattcacc ggaaatacca taacctatga ggatgttttc
      301 accggcagcg atgtcacttg gaggtacagg aacacggagt tgaaagagga aatcatcttg
      361 agtaatgcga ccaagacggt tttgcagaat catccaccat cactgtatag tctgaataat
      421 gagagttcgt atcttgtgtt catcactaaa ttggatcacc agaatctcaa cctgtataac
      481 gcgtcaggga tgctcaccgg aaatgttatg atctccgatg taggtgtcga tttcaaggat
      541 gtgttcggac agttcaaatg cgccctaccg ctgggtgagg cttacgagct gaacaatgaa
      601 tcggtacgac agaaactgac gtatcgtatc gtgcacctga acggcaacac ctatcttctc
      661 tctgggttaa aagcttctga tttgaatacg atgacgttcc ctgtcgtgat agacccgaca
      721 gtgaccctct attcaacctt cagcgacggc tatatctaca actatggaac gaattataat
      781 accgcacaat cctcttcata cggcatggaa agcagctctg caacatacat cacgatcgga
      841 cagaagaagg taagtaccat gtatttcatc aaccgagggt tcgtgttctt taacacctcc
      901 gtagtaccgt tcaacgccct tcttgacaac gcaacactcg ccctgtacaa aaaagatgat
      961 aactcaacca cggatttcga catcacgatt caagacgggc agccgaccta cccgcatgac
     1021 ccacttgaat ccagtgacta caacaaaaac cattactctg gaaacattgg agcattgaac
     1081 acctcaggtt ttacaaccgg atataacaat atcacactga acaaccttaa atgtatcaat
     1141 acaaatggaa cgaccaaact ctgtctgcgc agcagtcgag acatcaatgg cacaacaccg
     1201 acagggaacg aatatattaa tgtgttttca tccgattttg gtcttattgg ttgtctgccg
     1261 aaattgatca ttgtctatcg gaatcaatcg aagatcaaga acaccgggtc gaccgacata
     1321 caaggatata tcctcatgca agtgcatttc tacgacgagt ctcagtgtga atgggtgtgg
     1381 gacagtgatg ttatcgatga gcctaaacca cgaaccatca atagcaacag tcaattcgga
     1441 cttgacatga tcttcaatga ccggatcaga gcatcagatc ttcaacatgg agcgggtacc
     1501 tatcgagttt acactgcatt cagagatcca gagggaaaca tcctgagaac aaatgatgat
     1561 ttggaccttg aagcctggtg gcaattcaac aaaacataag taacgaagcg atcctcattg
     1621 actaaaacaa cggaagtcag gttattactg cctgattttc catactatca ttttttttag
     1681 ttattgataa tatgtgcaaa gctataatta atgcatctag aatggaagcg gttagcccta
     1741 ctaattgggt catctagacc atatcaggcg ttccttaata atatgagcga aaaaggaaaa
     1801 aaacctgaac gggaaatgag atgaacgacc ctttactagt aaacaggtgt tcaaatcgtt
     1861 tttttatagt tgcgactggg aatactgcgt ggtctgaaac gaagtgaagg acccgcagat
     1921 gaaaagtcgc atctctattt ttctcccaac aaaacatatc tcttttttgt cgcacgcggc
     1981 aggcgttcgc ctgccctata acggtgcgac aggagcaatc aatttatgga taatgttgta
     2041 attagctgta gctctaaaaa gattgcgggt gacacccgta acaactgggt gaccctgatg
     2101 ttcgtcaccc acaacagata caactgtttt cgaaagcaaa tctatattga catctgtatc
     2161 caagcgttcc atgacctaga acgctttgga ttcgagttcg gtgattttgg cttcgcactc
     2221 aatcatgttc acttcttagt gaatatccca aaacgatatt cggttcaaac cgctgaaatc
     2281 atgcttaaat catacactgc ccggaaaatg tttgaaacct ttccaggttt cagaaagcga
     2341 tacccacgcg gtggcttctg gagcggatac gatcattacg aatcaaccgg atggaaaaac
     2401 cttgaacaat caagtgtata catccgagat caatctcgac atcacaatgc atacgttgtt
     2461 gatgatcatc agcaacaatt acctaacgta gcttgaccgc cgagtgagat acggcaacgt
     2521 ccgatagggc ttgccgagga acgaaggcgg aacgggatca taagggcggc gcccttattt
     2581 cattacttac tcattcctct cctgtaagac cgttaaaatt agtgcagggg acgagatttg
     2641 aactcgcgga cctctacagg acaaggccct caaccttgcg ccgttgacca tgcttggcta
     2701 cccctgcaag tgttttttcc ggtaaatact agatactcta tatagctttt cgggtacctg
     2761 cccttaaata ttatccaggt ccatgccgca tcgttgacta agctcacgat gtaacgccat
     2821 taacctttcc gcatgcttca tctgtccttt ctgaagtgct ttttcgactt ctatttcaat
     2881 cctgtgctca tgatcgtttt caatgagatt atctgcatga gccacgattt tttcttctaa
     2941 cgtgatgggt atgtaatctt ttggtggtaa tccaaggatg attgcctctt cttttggaat
     3001 tcctgcacct agatgtcgtt cgatgatagt gatgatactt tctggtaaac ctaattgagt
     3061 agcgattttt gctccttcca ccccgtggag gatcccctgg gttttcgatc gtccgatgtc
     3121 atgaagcaat gcgcctgctt cgacgagttg aatatttgca tgggctttct tcgctattct
     3181 gaccgcgacc ttccgtacag ctttacaatg gttgataact tcatcggaac accctgcttc
     3241 tcgtaacaat cgcaggcact gtccaggatt ggggatcacg gtcatgattt ttcctttgaa
     3301 agaacctttt tccctttaac acagggaata atttgaatgt ttccatcgaa cagttttttt
     3361 tgccacgctg cttttgtaaa acggtcaagg aacaatgcaa gggcagcgac ctcagaatgt
     3421 ggttgatttc cgactgaaat gttcatgtca gctatctcat aaaaatatgg agggaccttt
     3481 tcagccccaa cgatgatgag cagatcgcga ttggtatcaa gatgctgtat tgctttttct
     3541 agctcttcgc cgtacatcgt aagatggaca atggctcctt tccatgattt gatgattttt
     3601 ttatgatcga tgccggtttg tacctggaaa tttccaccga atcgtttgca gacggattgg
     3661 atatttttct taattttttc atctttcgtt gtgatataga tgatgtctgc gccgaatgct
     3721 ctagcgacca atgcaacatg cgtggtcact cgtttgtccc gttcgattcg atgtccgatt
     3781 cttagcactg ctatcactgt tatcaaccat tccttgtgag acggaaacga aaaagagtct
     3841 taaaaacact ccgatatcga ccgtgtttct ctgcgatcta cttttttttc tttagcagga
     3901 taaaatcaac ttttttttcg gtatcaatcc ctatttgagt acgtcttgtt gttgattctg
     3961 attgtcctgg tcttatatta tcgatgttct gttcgattgt ttcagcattc tcccaggtgg
     4021 ttcgtcttat gggcggtttc tgttgaaatg aaggtgtttt ctgttgtttt tgtgcaaatc
     4081 cctttgaata aagtgtttcg ttatactcct tgattggtac ttctttaggt tctatttttg
     4141 tctctggttc gatttttttt tcatctgaac atcggatttc ttcccttgtt acattatctt
     4201 cgacgtgtgt ttcttctttt gtctttttac gctgaaacaa cttcaatttt accaacggaa
     4261 tcctcctgag ctatcgtttt cttggagagg ttatcttatc catacttgat aaggttttcc
     4321 taaaggagaa tcccttgagc tctagaaggt gaacgaaaag catttatccg tgtcctaaat
     4381 tcagacttat accttttgag ataaactggg ctagtggtct agttggttat gacatcgctc
     4441 tcacacggcg aaggccgccg gttcgaatcc ggcctagccc atactggtct acaggagaaa
     4501 ttttgttaga ttgattgatt tttgccaaac aatctatttt tgtctcctct cccctctcgc
     4561 atatattttc tatattttga gatctgaggg cgagattgaa tagattgcgt ttccctaatt
     4621 gcctcttgag acagtctgcg gtgtggatgt atttactcgt gtttgtaatc ttcgtgtggc
     4681 caagcatttt tgaaacaaat ttgatatccc ctgtatagtc gtagagataa gacgcgtagg
     4741 tatgtctcat tgtgtagaga tggaagtatt ccccagcgat cctcttacct gctgatgtga
     4801 gatgatttgt tatgtatctt cctgttattc gttttccatt tggttggaga aataaagcat
     4861 ttcctgagtt gctgttttct accttatgtc tccaattatc tctgtaattt cgtggcgtct
     4921 tgaacactgg tgatgataga actgtcttat tgaatggaat gataatcctg gtcttgtttc
     4981 gtttcttttg ttcgtggact gttaaggttc ctgtgccatc gtcattaaga tttaaatcct
     5041 cactgttgag attgcagatc tcgcttggtg gcctcattcc gaacatgaaa ccaatgaaag
     5101 ctacagattg taagagtaca ttctcgtatt tgtccccacc ataatcatgg gagaaaaatt
     5161 tgtttactgt ccctgggaat ggtatctgaa tgttatcttc attcactgtt atcggtggcg
     5221 ttttgcagac tattttccat ctgtccgtat cgattccgta ggctctgaga aacataacaa
     5281 tagctttttt ctcatgggct aaagcaaaag gtgtagcgtt ttctatttca atccggtaat
     5341 ccatgtgtct gatgaattgc tctggtgtta ggtttctgaa atcgataggc actggagatt
     5401 tttccataaa acgtgcgtat cgtagatttt ttgttattgt cgcatctgag agaaattgtc
     5461 ggatcttcgc atgaatgccc acttcgatga aagaggctgt tttagggtca agatatatgg
     5521 gaaactcagt tcttttgatg tttttcagct catattccag tttcaccatt tcctttccat
     5581 ttggaaaatc tttgttgata tcctggccta atctgtttat aatttctgtc catttttctg
     5641 tttgacctcc acccaaatca aaagacttgg tggatttcac atcagttgaa tctattttag
     5701 gattttcgtg aacagatttc aattaaaggt gacctcccaa aagattaatt atacagaaat
     5761 tgttgttcca attcaaatgt ggtttaaaac acgatatgat gacaaagaag ttgaatgtca
     5821 attttgtcat aaaacgacaa aaccgattat tgaaagaaat tggatggata ttggatttgg
     5881 aatggggcct ggtccttttt ttggacgagc gggatcgagt tccgctagta aaccaaatag
     5941 gttcgttctg atttgtccta attgtaaagc tcttattggt gttaaaaaat aaataatcac
     6001 ttgatttttt atttctcatt gattctcctt aataaaaacc ttaaaattct ggggggagga
     6061 ttgtttaccc catcgttgaa tataaccact tgttcgacgt cgtttcttat ctttacttcc
     6121 ttccggccgt ccaagttgtt ttccttgttt tcgcgctcga gcaagcccga gattaatcct
     6181 ctctttgatt a
//
LOCUS       DATMXJ010000201        10491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_40902, whole genome shotgun sequence.
ACCESSION   DATMXJ010000201 DATMXJ010000000
VERSION     DATMXJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40902"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VN377_05390"
     CDS             <1..341
                     /locus_tag="VN377_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27850.1"
                     /translation="PCLSQMFELKKISENYSTDDVAILSIDVWVSNGETSTMLQDLIN
                     EFKNQVNVTLDWTFGLDDPEGTIENAYASGGVPSLYIYDKKGNIYYSHVGYESYADLS
                     TKIDEVLIKN"
     gene            359..862
                     /locus_tag="VN377_05395"
     CDS             359..862
                     /locus_tag="VN377_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27851.1"
                     /translation="MKNERKIIPIIVIMSIIGTIAGSVAAAGVTFGTITVTPSQPAAL
                     STVTISVPISGEAPSEVRVTVEECNGRTGICYPDIQNVSMPLISSSIYRADVTLKHTD
                     ATYITVNIVAKISGVWQESSGKRVNFSENPDGNDGDGKKSPGFEVVVFVIAIGVILIL
                     IGRKRVK"
     gene            859..1683
                     /locus_tag="VN377_05400"
     CDS             859..1683
                     /locus_tag="VN377_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWR27852.1"
                     /translation="MKLQIKRLISQIILFFSANLGYPGYPGLKTGFCYPFFYCQACPA
                     ATSACPLRSLEQSVDKGSFTWRLFLFPLLIIGFLGVLTGRAVCAWACPIGLLQRGTGR
                     VARKVKKTQLARKLGAHKAEKYFRYTKYVLLISLVFVTSYLIGFIFTDICPVGFLTGT
                     IPVLILNPGKFIPNFFFPVALVIFALFIALIFLIERGWCRYFCPVGALLGPFNKISFY
                     HIAVKATECVHCNVCSYECPMGIDVPNMHRDPECILCGKCVTACPSELITFERKGR"
     gene            1680..2324
                     /locus_tag="VN377_05405"
     CDS             1680..2324
                     /locus_tag="VN377_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27853.1"
                     /translation="MKKFFALQVCALLLVSGTLGGMFALTPIYDEVRSGIVLVLCLSC
                     LKLEPNTLADFTFQTADGQPHPDFILENLSHGPVFLHYSGDSCAGCDVMYPVVKDLFS
                     IEFGKQDMFSSIVNFENSSVAYIYVNIHHTIPELRDAQPVYDKDHVAGIPMFTIVTLG
                     YDNGKVKPKYTSVYGTLATYGCDTDAQRLVFLQQLVRQSIDMYNENEAGYPHHP"
     gene            complement(2366..2530)
                     /locus_tag="VN377_05410"
     CDS             complement(2366..2530)
                     /locus_tag="VN377_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27854.1"
                     /translation="MKVVLCNDGVCVPPKCPVVDVRSDKVIIGEKTNVCTLTRAQFEI
                     LRQKIITGEI"
     gene            complement(2620..4005)
                     /locus_tag="VN377_05415"
     CDS             complement(2620..4005)
                     /locus_tag="VN377_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27855.1"
                     /translation="MNPFYNPVFLGEILKRYLVDVDRLERMSNESLHRYQDRRLRRLV
                     RFADTVPLYHEKFKQAGIHPADISGIDDLQKLPFVTKDDFKRYYPNGIISSKIKKDSL
                     VEVSTSGTTGKVLPLYVDLLDIVMGLFAYMRTLREYDMSIWKNRITIIGDFAPHTAES
                     GYVTRGLQPRFNLTRMFKNIQWLNTNDDPKKVIASINEFHPEFIGGYVGMLGHLALLK
                     ETGLGKDISPQYIAATGSVLDSFLKSYIEKTFQAKVFEVYGATESGLIGYQCKQGSYH
                     LMSDLVYAEFLRDGHPVGSGEAGEMIITKFHGKGTPILRYNAINDIVAPSIGTCPCGK
                     AGQLIKKIYGRNDLALYLSDGTVLLPSAFSVIYSKLLYQLRTNKVKNTKIVQHDFTTL
                     EIQVVIDETLRTIGPSVEEIFSFISKGFQEKMGSNVTVTVKETASVDTSQGPRIVSLI
                     DKKNYLINRYV"
     gene            4079..5404
                     /locus_tag="VN377_05420"
     CDS             4079..5404
                     /locus_tag="VN377_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27856.1"
                     /translation="MNILIVETVWMGGARYKFLEKTLLTTFSILPTLQARELAAITPK
                     QHQVSVINERYSQIDYNTGYDVVLINYVTSTAPRAYAIADAYRKKGVLVVLCGFHASG
                     LPEEAKQHADSVLIGRNEAGWLAVLQDIEQKKLQPYYHAPPYSISQNLPPTNVHLPGF
                     ILTGALEATRGCPYHCEFCPETNIQGGNIFYKRPIPEVIEEIKKIPQKTLMFYDTSLT
                     IDPPYTKELFKNMKGLHKRFFCNGNADVLAHDAELVRLSKEAGCVAWLVGFESLCQNT
                     LDRVGKKTNKVDEYGQAIQNIHKNHMAVIGDFMFGFDTDTPDMFEKTLKEIKELNIDI
                     ADFSILTPFPGTPLFTKLDAEKRILTKDWKYYNMGHVVFTPKQMTPDELLQGVRFMYS
                     EFYRPSYTIKRIALNFNRGPYPFFVVFARNMVATLAARRLYTSKNPKKQ"
     gene            complement(5410..6888)
                     /locus_tag="VN377_05425"
     CDS             complement(5410..6888)
                     /locus_tag="VN377_05425"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27857.1"
                     /translation="MRLIGKFIVIIILIGILLSASAYIVLYTDENGGPDSKSPQITSI
                     SGNFTVTAGQTATIIAVFTDNVNVTQATLYYTIEGSATWNSLSILNGSASINIPSNST
                     TDYYYYVTVDDAVGNGPVGAPSTDGSVFFIITVEPNNGEGDFTHTVFIEESTSITCRY
                     CPNVGAILEKLDSSHAYRFYYVSLIMENSAAAEYLSSNYNRVGDPTVYMDGGYKVILG
                     GLNPESNYTSAIQTAENRVVPKIKVTANAEYKNTTNAIETNILVVNGEQSTYSGRLRV
                     YLAEIISSQYNDYNGLKYRNAFVDFIINEDISVSANSDGTFSASWPVGNLDYENLKII
                     AVVFSSIENSAFSDPPTNAYPFTAHYADASNETYVVKGPRNLPPEVGILSPQNGKIYI
                     RGNLRGNFLYKNGLLKNTWLIGKASIDTYAKDDSGITRVEIYVNDKLVANMTAPPYNW
                     TLPFKLIKKPLIPKDYTIMVKAYDDTDKTATASIVVKAWWAF"
     gene            complement(7005..7838)
                     /locus_tag="VN377_05430"
     CDS             complement(7005..7838)
                     /locus_tag="VN377_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HWR27858.1"
                     /translation="MTSSQITSRNITTTEIKRFQKKVFSFYEKNKRDLPWRKTIDPYK
                     ILLSELMLQQTQVKRVILYYEKWIARWPTIDALASASLPEVLHMWMGLGYNTRAKNLH
                     TTARKIVATFNSDVLQAMKQYKELPGIGKYTSQAVQIFSSNSDLVTVDTNIRRILISE
                     FKLPIKIADKELWLLAETCLPLGESRDWHNALMDYGALHMTAQKTGIKPKTQQTQFEG
                     SNRQIRAQILRCLLQKSMSFSELEKTVGIGQMRLGAILDKMMNEKIIENKNKTYHLNE
                     A"
     gene            7958..8869
                     /gene="cysD"
                     /locus_tag="VN377_05435"
     CDS             7958..8869
                     /gene="cysD"
                     /locus_tag="VN377_05435"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWR27859.1"
                     /translation="MKRYYLNHLQKLEAESIFVIREVAAQFERPVLLFSGGKDSIVLG
                     YLSKKAFAPAKIPYPLLHIDTGHNFPEVLEFRDAFVKYLDVRLLVGSVQKSIDAGRVK
                     DETGKFATRNYLQSTTLLDTIHEHKFDAAIGGGRRDEEKARAKERFFSHRNDFGQWDP
                     KNQRPELWDLYNGRKHHSEHFRVFPLSNWTELDIWQYILKEQIRIPSIYLSHTRDCII
                     RDGVIYAYSDYLHLNEEEKKKVQPMTVRFRTVGDMTCTGAVQSTARTVEEIVQEVAAY
                     RVSERGNRADDRRSENSLEDRKKEGYF"
     gene            8871..10166
                     /locus_tag="VN377_05440"
     CDS             8871..10166
                     /locus_tag="VN377_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWR27860.1"
                     /translation="MDEKTYLNMDLLRFTTAGSVDDGKSTLIGRLLYDSKTLFEDQLE
                     NLERLSQQRGEELNLALLTDGLRAEREQGITIDVAYRYFATPVRKFIIADTPGHIQYT
                     RNMVTGASTANLALILVDARNGVTEQTHRHIFIASLLQIPHFVICINKMDLVDYKQDV
                     YDRIKTEIEDFISKLSVPDITFIPISALLGDNVVNPSKNMEWYHGPTLLYHLEDVHIG
                     SDENLVDCRFPIQYVIRPRSDKYHDYRGYAGKIASGVFKPGDSVIVLPSGFTTTIKTI
                     EDPRGEVAEAFPPMSVVMQLADDLDISRGDMLVRPHNIPFISQDLDCMVCWMNTKPLI
                     QGTRYIIRHTTRETKGIVKEIQYKIDINTLHRIPEVTSLGLNEIGRITIRTAQSLFFD
                     SYQHNRITGSIILIDETTNETIGAGMIREPSVKVPEKKK"
     gene            10180..>10491
                     /locus_tag="VN377_05445"
     CDS             10180..>10491
                     /locus_tag="VN377_05445"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWR27861.1"
                     /translation="MKKHLGFTIWFTGLPCCGKTTIANQVAVILKKRDYLVEQLDGDL
                     IRQNFSSDLSFSKKDRDENIRRATFLAKMLSRNNVVVLASFVSPYRKQRRKTRKEIKN
                     FV"
BASE COUNT         2911 a         2285 c         2328 g         2967 t
ORIGIN      
        1 aaccatgctt gtctcagatg tttgagttaa aaaagatctc agaaaattat tctactgatg
       61 atgttgcaat cctttcaata gatgtctggg tatcaaatgg agaaacatca accatgcttc
      121 aagacctgat caacgagttc aaaaaccagg tcaatgtgac cttagactgg acatttggct
      181 tggatgaccc agaaggaact attgaaaacg cctatgcctc cgggggagta ccatctcttt
      241 atatctatga caaaaaagga aatatttatt actcccacgt gggttatgaa tcatacgcag
      301 atctttcgac caagattgat gaagtgctga taaaaaatta gagggagaac cgaaaactat
      361 gaagaacgaa agaaaaataa taccaatcat cgtaatcatg agtatcattg gaacaattgc
      421 tggttcagtg gccgcagcag gtgtgacgtt tgggactatc actgttactc catcacaacc
      481 cgctgcgtta tcaactgtga ccatctcagt accgataagt ggcgaagcac catcggaagt
      541 acgggtgact gtcgaggaat gcaacggaag aaccggtatc tgttatcctg atatccaaaa
      601 tgtctcaatg ccgctcatca gctcaagtat ttatcgagcc gatgtgacat tgaaacatac
      661 agatgcgacg tacatcacgg ttaatattgt cgcaaaaatc agtggtgttt ggcaagaatc
      721 ctcaggaaag agggtgaact tttctgagaa tccggatgga aacgacggtg atggtaagaa
      781 gtcaccagga tttgaagtcg tggtgttcgt gatagcaatc ggtgtcattt tgatactgat
      841 aggtcgtaag agagttaaat gaagttacaa ataaaacgat tgatttctca gattattctt
      901 ttcttttctg ctaacctggg ttaccctggg tatccggggt taaaaaccgg tttttgttat
      961 ccttttttct attgccaggc atgccccgca gcgacctccg catgcccgtt acgatcattg
     1021 gaacaaagcg tcgataaggg cagttttacc tggaggttgt ttttattccc tcttcttatc
     1081 atcgggttcc ttggggtttt aacaggccgc gcagtgtgcg cctgggcttg tcccatcgga
     1141 ttattgcaac gggggacagg acgtgtcgca aggaaagtga agaaaacaca acttgcgcga
     1201 aaacttgggg cacataaagc agagaaatat ttccgttaca cgaaatatgt tcttctcatc
     1261 agcctcgtgt ttgtcacctc ctatctaatc ggattcatct ttaccgacat ctgtccagtt
     1321 gggtttctga caggaaccat cccggtcctc atcctgaacc ccgggaaatt tatcccgaac
     1381 ttttttttcc cggtcgcctt agtcatcttt gctttattta tcgccctcat cttccttatt
     1441 gagcgaggtt ggtgtcggta tttctgcccg gttggtgctc tcctgggacc gtttaataaa
     1501 ataagttttt atcacatcgc agtcaaagca acagagtgcg ttcactgtaa cgtctgctcg
     1561 tatgaatgtc cgatgggaat cgacgtacca aacatgcatc gagacccaga gtgcatttta
     1621 tgcgggaaat gcgtgactgc ctgtccgagt gaactgatca cctttgagag gaagggacga
     1681 tgaagaaatt ctttgctctg caggtctgtg cactactcct ggtttcagga acgcttggtg
     1741 gcatgttcgc tctgacacca atctatgatg aggtacggtc cggtattgtg cttgtgctgt
     1801 gtttaagctg cttgaagctt gagccgaaca ccttagcaga tttcaccttc caaaccgcgg
     1861 atggccagcc tcacccagat tttatattgg agaatttatc ccacggacca gtatttttgc
     1921 attatagcgg tgattcatgc gccggctgtg atgtgatgta cccggtggta aaagacctct
     1981 tttcgatcga gtttggaaaa caggacatgt tctcttccat cgtcaatttt gagaactctt
     2041 cggtcgccta catttatgtg aacatccacc acaccatccc cgaattgaga gatgctcaac
     2101 ccgtttacga caaagatcat gtcgcgggaa tcccaatgtt tactattgtg acgttaggtt
     2161 acgataacgg taaagtaaaa ccgaaataca cctccgtata tgggacgtta gcgacctatg
     2221 gatgtgatac cgatgcgcag cgactcgtgt ttcttcaaca gctcgttcgt caaagtattg
     2281 atatgtacaa cgaaaacgaa gcagggtacc cccatcatcc ataattcttt ttttaaaaaa
     2341 aaaaaagcga aaacagacga tattcttaga tctcaccggt aataatcttt tgtctgagga
     2401 tctcgaattg tgcccgggtt aacgtgcaca cgttcgtttt ttcgccaata atcaccttat
     2461 cactccggac atccacgaca ggacatttcg gagggacaca tactccatca ttgcataata
     2521 caactttcat attatccctc tgattatcaa ttgttaagtc tctattaggc tttgaacctt
     2581 taaatatctt tcgacagagg agcagaaaaa ggactctgtt taaacataac ggttaatcaa
     2641 atagtttttt ttatcaatga gtgatactat gcggggtccc tgcgaggtat caacagaagc
     2701 agtttctttg acggtgactg ttacgttaga ccccattttt tcttgaaatc ctttcgatat
     2761 gaaactaaaa atctcctcca cagatgggcc aatcgtgcga agtgtctcgt caataacgac
     2821 ctggatttcc agcgtcgtga aatcatgttg aacgatcttc gtgttcttca ccttgtttgt
     2881 tcttaattgg tagagtagtt tactataaat cactgaaaac gcagacggaa gcaacactgt
     2941 accatctgat aggtacaacg caaggtcgtt tcgcccgtat atttttttga tcaattggcc
     3001 tgctttaccg cagggacatg ttccaattga tggagcaacg atgtcattga tagcgttata
     3061 gcgaagaatc ggtgttcctt ttccatggaa ttttgtgata atcatctctc ctgcttcgcc
     3121 tgagccgaca ggatgtccat ctctaaggaa ctctgcgtag acaaggtctg acatcaaatg
     3181 gtagcttcct tgtttgcatt gatagccaat caacccggat tctgttgctc cgtagacttc
     3241 aaagaccttt gcttgaaatg ttttttctat gtaggatttc aggaaagaat ccaggacaga
     3301 tcctgtggca gcgatatact gaggtgagat gtctttgccc aatcctgttt cttttaacaa
     3361 ggcaagatgg ccaagcatcc cgacgtaccc gccgatgaac tctgggtgga actcattgat
     3421 ggaggcgatg actttctttg ggtcatcgtt cgtgttcagc cactggatgt ttttgaacat
     3481 tcttgtgaga ttgaaccgag gctgaagccc tctggtgaca tacccgctct ccgcggtatg
     3541 tggcgcgaaa tcaccgataa tcgttatcct gtttttccat atactcatgt catattcacg
     3601 caaggtccgc atatacgcaa acaaacccat gacgatatca agcaaatcga catacaatgg
     3661 aaggactttc cctgttgtgc ctgaagtaga gacctcgaca agggaatcct tttttatttt
     3721 tgatgagata atcccatttg ggtaatatcg tttgaaatca tctttagtca caaaagggag
     3781 tttctgaaga tcatcaatcc cggaaatatc ggccggatgg atccctgcct gtttgaattt
     3841 ctcatgatac aaaggtaccg tatcggcaaa ccggacaagt cgtctcagtc gtctgtcttg
     3901 atagcgatgt agcgattcat tgctcattct ctccaaccgg tcgacatcca ccaaatatcg
     3961 tttgagaatt tcaccaagga acactgggtt atagaacgga ttcatacagc tcccaaagtt
     4021 gacaggagat aggtacaatt ccttaaatag ctgtcccttc attctccaca gaagagcaat
     4081 gaacatactc atcgttgaga ccgtttggat gggaggcgca cggtataaat tccttgaaaa
     4141 aacattgctc acgacattct ctatccttcc caccttgcaa gcacgtgagc ttgccgcgat
     4201 cacaccaaaa caacaccagg tatccgtaat caatgaacga tactcccaga ttgactataa
     4261 tacaggctac gatgtagtcc tcatcaacta tgtgacttcg acagcacctc gagcctatgc
     4321 cattgctgac gcataccgga aaaaaggtgt cctggttgtg ctctgcgggt ttcatgcctc
     4381 aggactcccg gaggaagcaa aacagcatgc agatagcgtt cttatcggga gaaacgaagc
     4441 aggatggtta gcggttctac aggatatcga gcagaaaaaa ctacagccgt actatcatgc
     4501 accgccatac agcatctctc agaatctccc tccgacaaat gttcatttac cgggatttat
     4561 cctaaccggt gcgctcgagg cgacacgcgg gtgtccctat cactgtgaat tctgcccaga
     4621 gacaaacatc cagggtggaa atatattcta caaaagacca ataccagagg tcatagaaga
     4681 aataaaaaaa atcccacaaa aaacactgat gttttatgat acgtcgctca ccatcgaccc
     4741 gccctataca aaggaattgt tcaaaaacat gaaaggactc cataaaagat tcttctgcaa
     4801 tgggaatgct gatgtattgg cccatgacgc ggaactcgtc cgactctcaa aagaagcagg
     4861 atgtgtcgcc tggcttgtcg gatttgaatc cctctgtcaa aacacccttg acagggtagg
     4921 caaaaaaacc aacaaagtcg atgaatacgg acaagcgatc cagaacatcc ataagaatca
     4981 tatggcggtc attggagact tcatgttcgg gtttgatacc gatacaccag acatgtttga
     5041 gaagacgctc aaagaaataa aagagctgaa catcgatatc gctgatttct ccattcttac
     5101 cccgttcccg ggaacacctt tgtttactaa acttgatgcg gagaaaagaa tcctaacaaa
     5161 agattggaaa tattataaca tgggacatgt ggtcttcacc ccaaagcaga tgacgccaga
     5221 cgaactactc caaggcgtac gattcatgta ctctgagttt tatcgaccat cgtatacaat
     5281 aaaaagaatt gcactgaact tcaatagagg tccttacccg ttcttcgtcg tcttcgcgcg
     5341 aaacatggtt gcgacactcg ccgctcgacg attatatacg tcaaaaaatc caaaaaaaca
     5401 gtaataaggt tagaatgccc accaggcttt gacaacgatg cttgcggtag cggttttatc
     5461 ggtgtcatca taggctttta ccatgatggt atagtcctta ggaatcaatg gttttttaat
     5521 taatttgaaa gggagcgtcc aattatacgg tggtgcggtc atgtttgcga caagtttgtc
     5581 gttcacatag atttcgaccc tagtgatccc ggaatcatcc ttcgcgtacg tatcaataga
     5641 ggctttcccg atgagccagg tgtttttcaa taatccattt ttatataaga aatttccacg
     5701 gagatttcct cgaatgtaga tctttccgtt ctgaggtgat aggattccaa cctccggggg
     5761 aaggttccgt ggacccttta ctacatatgt ctcatttgat gcatcagcat aatgagcggt
     5821 aaacggatag gcgttcgttg gagggtctga aaacgcgcta ttttctattg agctgaacac
     5881 gactgcaata atcttaagat tctcataatc aaggttcccg acaggccaac tcgctgaaaa
     5941 cgtcccgtca ctgtttgcag aaacgctgat atcttcattt ataatgaagt cgacaaacgc
     6001 gtttctatat tttagaccgt tataatcgtt atattgtgaa gaaataattt cggcgagata
     6061 cacccgtaat cgtccggaat aggtggattg ttctccattt actactagaa tgtttgtttc
     6121 tatcgcattg gtagtgttct tgtattccgc gttagccgta accttaattt ttgggaccac
     6181 ccgattttcg gctgtttgga tagcactggt gtagtttgat tctgggttta gaccaccaag
     6241 aataactttg taaccaccat ccatatacac agtcggatct ccaacgcgat tatagtttga
     6301 tgagaggtac tctgctgctg cgctgttttc catgatcaga cttacataat aaaaacgata
     6361 agcatgagag gagtctaatt tctccaaaat agctccgaca ttcggacagt accgacaggt
     6421 tatcgacgtc gattcctcaa tgaacacagt atgggtgaaa tccccttcgc cattgttcgg
     6481 ttctaccgtg atgatgaaaa acacagaacc atcagtcgaa ggtgcaccaa ccggaccgtt
     6541 tccgacagca tcatctacag tcacatagta ataataatca gttgtggaat ttgatggaat
     6601 gttgatgctt gcacttccat tcagaatgct tagggagttc catgttgctg acccttcgat
     6661 agtataatac aaggttgctt gggtgacatt gacgttatcg gtaaaaacag cgatgatagt
     6721 ggctgtttgg ccggctgtga cggtaaagtt acctgagata cttgtaatct gtggagattt
     6781 ggaatccggg cctccatttt catcagtgta aagaacaatg tatgctgatg cactaaggag
     6841 aattcctata agaatgatga ttacgataaa ttttccgatg agcctcatat cttgtaatta
     6901 atatacactt gttttatata ttaaatttac tgagacagat ttaagaggag aatgtcgaaa
     6961 atatatagag aaaatcatca attctcctcg ttaaaagatt caggttatgc ttcgttcaag
     7021 tgataggttt tattcttgtt ttcaatgatt ttttcgttca tcatcttatc aagaatcgct
     7081 ccaagtcgca tttgtcctat tccaactgtt ttctccaact cagagaacga catcgatttc
     7141 tgaagtaaac atcgtaaaat ctgtgctcga atctgtcgat tggagccttc gaactgtgtt
     7201 tgctgtgttt ttggtttgat accggttttc tgtgcggtca tatgaagagc accataatcc
     7261 attagcgcgt tatgccagtc ccgactttct cctaatggaa gacatgtttc ggcaaggagc
     7321 cagagttcct tatcagctat tttgatcgga agtttaaatt cacttatgag gatccttcgg
     7381 atattggtat caacagtgac aagatcagaa tttgaggaaa aaatttgcac agcttgagat
     7441 gtatattttc cgataccagg gagttctttg tattgtttca tcgcctgaag tacatcacta
     7501 ttgaacgtag caacaatttt cctagcagta gtatggaggt ttttcgctct ggtgttatac
     7561 cctagaccca tccacatgtg tagtacctca ggaagggacg cagaggcaag agcgtcgatc
     7621 gtgggccatc ttgcaatcca tttctcataa tacaagatga cacgttttac ctgtgtctgt
     7681 tgtaacataa gctccgagag cagtatctta taggggtcga ttgtcttccg ccaaggcaaa
     7741 tcccgtttat ttttttcata aaaagaaaag actttttttt gaaaccgttt gatttccgtt
     7801 gtagtgatgt ttcttgaggt gatctgcgaa gacgtcatat ccagcccttt tttgatgaaa
     7861 atctgggtat aatacccatg gtcttaatat gattttattc ttcatttatt atttggtaag
     7921 gtttttgaag gtttgttgta taaaggatat cccccttatg aaacgctatt acctgaacca
     7981 tctccaaaaa cttgaggcag aatcaatctt tgtcatccgt gaggtcgcag cacaattcga
     8041 acgtccagtg ctgctttttt caggtgggaa agactcgatc gtccttgggt atctatccaa
     8101 aaaagccttc gctcctgcga aaatcccata tcccttactg catattgata ccggtcataa
     8161 ctttcccgag gtcttggaat tcagggatgc gttcgttaaa taccttgatg ttcgtttact
     8221 ggttggttcg gtccagaaat ccattgacgc gggacgggtg aaggacgaaa cagggaaatt
     8281 cgcaacaagg aattatcttc agtccaccac cttgcttgac accattcatg agcataaatt
     8341 cgatgcagcc atcggaggtg gacggagaga tgaggaaaaa gcaagagcaa aagaacggtt
     8401 tttctcccat cgcaatgatt ttgggcaatg ggacccgaag aaccaacgtc cggagctgtg
     8461 ggacctctac aatggtcgaa aacatcatag tgagcatttc cgtgtgtttc cgttgagtaa
     8521 ttggacggag ctggatatct ggcaatatat tctcaaagaa cagattcgga tcccctcgat
     8581 ttatctctca catacccgag attgcattat ccgggatggt gtgatctacg cgtactcaga
     8641 ttatcttcat ctcaatgagg aagaaaagaa aaaagtacag ccgatgactg ttcgattccg
     8701 tacagttggt gacatgacct gtaccggggc agtacaatcc actgctcgta ccgttgaaga
     8761 gatcgttcaa gaggtcgcgg cgtaccgtgt ctcagaacgt ggtaatcgag cagatgaccg
     8821 acgatcggag aattctcttg aggatcggaa aaaggagggg tatttctagg atggatgaaa
     8881 aaacctatct gaacatggat ctgcttcgtt tcaccaccgc tggcagtgtc gatgatggaa
     8941 aaagcaccct catcggtcgg ctcctctatg atagtaaaac cctttttgag gatcaattgg
     9001 aaaatcttga gcgtctgagc caacaacgtg gtgaagaatt gaaccttgcc ttgctgaccg
     9061 atggtctgcg agcggagcgt gaacaaggga tcacgattga tgttgcgtat cgatactttg
     9121 caactcctgt ccgaaaattc atcatcgctg atactcctgg acacattcag tatacacgga
     9181 acatggtaac cggggcatca accgcgaacc ttgcgttgat ccttgtggac gcccgtaatg
     9241 gggtcactga gcaaacccat cgtcatatct tcatcgcctc gctccttcag atcccgcatt
     9301 ttgtcatctg cattaacaag atggatcttg tggactataa acaggatgtg tacgaccgta
     9361 ttaagacgga gattgaggat tttatttcaa agctttcggt tccagatatc acctttatcc
     9421 ctatcagcgc gctccttggt gataacgtgg tgaatccgtc aaaaaacatg gagtggtacc
     9481 atggcccgac cctgttgtac catctggagg acgtccatat cggcagtgac gaaaacctag
     9541 tggactgtcg gttccctatc cagtacgtga tccggcctcg atcagataaa taccatgatt
     9601 accgagggta tgctggtaaa atcgctagtg gggtttttaa accaggggat agcgtcattg
     9661 ttcttccctc tggttttacg acaacaatca agaccattga agacccgcga ggggaagtcg
     9721 ctgaagcgtt ccctccgatg tcggtcgtca tgcagcttgc tgacgatctt gatatcagcc
     9781 gaggggatat gctcgtgcgt ccgcataaca tcccgtttat cagtcaggat cttgattgta
     9841 tggtctgttg gatgaacaca aaaccattga tccagggaac caggtacatc atccgtcata
     9901 ccactaggga gacaaaaggg attgtcaagg agattcaata caaaattgat attaacacgc
     9961 tgcatcggat accggaggtg acgtcccttg gacttaacga gattggacgg atcacgatac
    10021 ggaccgcgca atccttgttc tttgatagtt accagcataa ccgaattact gggagtatta
    10081 ttttgattga tgagacgacc aatgagacga ttggtgcagg gatgatacgg gaaccctctg
    10141 tgaaggtccc tgagaagaaa aaatagaggc agatgaagta tgaagaagca tctgggtttc
    10201 accatctggt ttacaggtct tccttgttgt gggaaaacaa cgatcgctaa tcaggtcgca
    10261 gtgattttaa aaaagaggga ctacctggtt gaacaactcg atggtgatct gatccgtcag
    10321 aacttttcaa gtgatctgag tttttcaaaa aaagacaggg atgagaatat cagacgtgcc
    10381 acgtttcttg ccaagatgct ctcccgcaat aacgttgtcg tgcttgctag ctttgtctca
    10441 ccatatagaa agcaacgaag gaaaacgcgc aaggaaatta agaatttcgt t
//
LOCUS       DATMXJ010000202         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_19868, whole genome shotgun sequence.
ACCESSION   DATMXJ010000202 DATMXJ010000000
VERSION     DATMXJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19868"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VN377_05450"
     CDS             <1..1221
                     /locus_tag="VN377_05450"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWR27862.1"
                     /translation="VLISANLSWTGGDPDNDTVTYDVYFGTTSPPEKKETHILNTSYD
                     PGTMNYSMKYYWQIVAWDDNNSSTGPQWEFTTEDKVNHPPNMPNNPNPTNTSTNIPIE
                     TVLTWDGGDPDAGDVVKYDVYFGKSIPPVNVSNNQSITSYTPVEDLAFNTTYYWKIVA
                     WDNNKASTVGPLWHFTTGKEAPLTVTIDKPTHGMFYFNDAEKLPLSGNNTIVYGKITI
                     IANVSATSDIDRVEFWVDGKQIANVTQEPYSVVWKPVIQFNSAFSLKRTITVIAFDSD
                     GNNATANITITKWRFHPLPWIIAGMAVASRFVLHTTVVGLFFNVQQSRFSVSFYALQA
                     HYKTVGPLQMRRGNIHFKHCTGGILIGPTTLTRIGPFHKFAIGSFTFIGNVNADKIGL
                     GGSLLSRILQRRSG"
     gene            1546..2088
                     /locus_tag="VN377_05455"
     CDS             1546..2088
                     /locus_tag="VN377_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWR27863.1"
                     /translation="MEFIDVIKARTSVREYGEKQVEDEKINYVLECARLAPSWTNKQC
                     WRFIVIKNKETIEQIGKSSLINRWLRTAPVLLIACADPTDSGSNNSQEYYMTDVSIAL
                     EHIILAATDAGLGTCWVAGFNEEKVKELLEVPKRIRIVALTPLGYPVGKKGITEQITK
                     TLLKANKRKTLDEIVHYEHW"
     gene            2136..>2683
                     /locus_tag="VN377_05460"
     CDS             2136..>2683
                     /locus_tag="VN377_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWR27864.1"
                     /translation="MAPSQDSQIPYAKKYFSLIILLPIIFWAFAFPFIKIGLEELSPI
                     NLTILRLFTTCGVFLVFLVFFPKKFTPIQRKDIVPIFLLGFFGLVVYHLGLNYGELYI
                     SPSVASLIIATIPVFTVIFATLLLKEKVTKKLAIGVPLSLSGVVIISLTGTSGNPFEV
                     MYLSAALAVLISAFVGAGYTIAG"
BASE COUNT          763 a          592 c          569 g          759 t
ORIGIN      
        1 gttttaatat ctgcgaattt aagctggact ggtggtgacc ctgataacga tacggtaacc
       61 tatgatgtgt attttgggac tacaagccca cctgaaaaaa aagagacaca tattttaaat
      121 acgagctatg atccggggac gatgaattat tccatgaaat attactggca gatagtcgct
      181 tgggatgata ataactcaag tactgggcca caatgggagt ttacgacgga agataaggtg
      241 aaccatccac ccaacatgcc gaataatcca aatcctacga atacatcaac aaacattcct
      301 atcgaaactg ttcttacctg ggatggtggt gaccctgatg caggggatgt cgtgaagtac
      361 gatgtgtatt tcgggaaatc aattcctcct gtgaacgtct caaacaatca gtcgatcacg
      421 agttataccc ctgttgaaga ccttgcgttt aatacaacat attattggaa aattgtcgcc
      481 tgggacaaca ataaagcctc aacagttggc ccgctttggc atttcacaac aggaaaagaa
      541 gcgcctctga cggtgacgat cgacaaacct acgcacggta tgttttactt caatgatgca
      601 gaaaaactgc ctttatctgg aaataatacc attgtctatg ggaaaatcac gattattgcg
      661 aatgtaagtg caacttcaga tattgatagg gtagaattct gggtagacgg aaagcaaatt
      721 gcaaacgtaa ctcaagaacc gtacagtgtc gtatggaaac cggtcatcca attcaatagt
      781 gccttttcac taaaacgcac cataacagta attgcttttg acagtgacgg caacaacgcc
      841 actgcgaata tcaccatcac aaaatggagg ttccatcctc tcccctggat cattgcaggg
      901 atggcggttg cctcacggtt tgtgctgcat accacggtgg taggactgtt cttcaatgtc
      961 caacagtcgc ggttcagtgt ctcgttttac gcgcttcagg ctcattataa aaccgttggg
     1021 ccactgcaga tgcgcagagg aaacatccac ttcaagcatt gcacgggtgg tattctcatt
     1081 ggaccgacga ctttgaccag gattggtccg ttccataaat tcgcgatcgg gtcgttcacg
     1141 ttcattggga atgtgaacgc tgataagatc gggcttggag gttcactact ttctcggatc
     1201 ctccaacgtc gatcaggctg aagcaatcca tccactctct aaaaaatctt aactcataag
     1261 aatcgagggg gatacgacct cttgacaacc agcgaattgc tggtttctca tttttttttg
     1321 aaatcgttga atcattcgtc tcatattttg accagcgaat ttctcgcccc cttagattca
     1381 acagaatgat caagtatttc gagatagata aggttcaatg gcccggttgg cttcatttcg
     1441 gatcccattt tataacggtg tcttatttat aaaaacagcc cattccatag aaaacatctt
     1501 ttataacacc actgcattac ccagtgaacc acccgaggcg caataatgga atttatcgat
     1561 gtgatcaaag ctaggacgag cgttcgtgaa tacggtgaga aacaagtcga agatgagaaa
     1621 atcaactatg tccttgagtg cgcacggctt gcgccatcct ggactaataa acaatgttgg
     1681 cggttcattg tcattaaaaa taaggagacc atcgaacaaa ttggaaaatc aagtttgatt
     1741 aaccgatggc tacggacagc cccagttctt cttatagctt gtgctgatcc taccgacagc
     1801 gggtcgaaca attctcagga gtattatatg acggatgtct cgatagcatt agaacatatt
     1861 atacttgcag ctaccgatgc tgggttagga acctgttggg tcgcaggatt taacgaggag
     1921 aaagtaaaag agctgctgga agttcccaaa cgaattcgca ttgtcgctct cactcctctt
     1981 ggatacccgg tgggcaagaa agggatcacc gaacaaatca caaaaaccct tctcaaggca
     2041 aataaaagaa aaacccttga tgagatagtt cattatgagc attggtagtt cctccatgct
     2101 tttaaataac aaatttcatt tgtccggaac aaaccatggc accatctcag gattcacaaa
     2161 taccttatgc aaaaaaatat ttttctttga ttatccttct tccaattatc ttctgggcgt
     2221 tcgcttttcc ctttataaaa atcggattgg aagaacttag tccgataaat cttactatct
     2281 tgcgtctttt taccacctgt ggtgtctttc tggtttttct tgtatttttc ccaaagaaat
     2341 tcaccccaat tcaaagaaaa gacatcgtac caattttttt gttaggattt tttggtctgg
     2401 tggtgtatca tcttggattg aactatggtg aactgtacat ttcaccttca gtagccagtc
     2461 ttatcatcgc gaccatccct gtgtttaccg tgattttcgc gacccttctt ttaaaggaga
     2521 aggtgaccaa gaaactcgcg ataggtgtac cactgtctct ttctggtgtg gtgatcatct
     2581 ccctgaccgg tacctccggc aatccgttcg aggtgatgta tctttctgca gcgctagcgg
     2641 tattaatctc tgcatttgtc ggggctggat acaccatcgc tgg
//
LOCUS       DATMXJ010000203         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_61882, whole genome shotgun sequence.
ACCESSION   DATMXJ010000203 DATMXJ010000000
VERSION     DATMXJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61882"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VN377_05465"
     CDS             complement(<1..249)
                     /locus_tag="VN377_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441886.1"
                     /note="signal recognition protein receptor; functions in
                     the targeting and insertion of membrane proteins; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWR27865.1"
                     /translation="MFDFLKKKLKLFEKNIEAEIESELKKEEKKEQKLQMEPQKQIVQ
                     TPTPTKSVEIPPAAPVIQKEKEPVVTRKRALRIQKPISE"
     gene            complement(272..691)
                     /gene="pfdA"
                     /locus_tag="VN377_05470"
     CDS             complement(272..691)
                     /gene="pfdA"
                     /locus_tag="VN377_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HWR27866.1"
                     /translation="MTQEEEITKNLTLIEYYKQQLESIEMQLQYLQSTLMDYQRAKMT
                     VEQLSAVDDNSEVLIPVGAGTFVSGSLKNASNVLIGVGAGIVIEKPADEAMGKLDERI
                     KRIQENLDKMASLGQKIQCDAEDLSHKTQQMMEESQR"
     gene            1048..1677
                     /locus_tag="VN377_05475"
     CDS             1048..1677
                     /locus_tag="VN377_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWR27867.1"
                     /translation="MRILAVADIHGAQYRLNLVLKNVASYTPDLVVICGDITQFGPGE
                     LATNFLNQIQIKTFAIPGNIDTFDVDQGINASNATNLDKRQVILQDVPFLGIGREPPS
                     QLSNLPIDDGTTKKPLKKLLKTSSILVTHYPPYKIQDKIFIGTHGGSKELRSLIDTYK
                     PRLVLCGHIHEDPGMATMGDTTVVNCSMGKRTEGALVEIGDTIKVSILD"
     gene            1940..>2454
                     /locus_tag="VN377_05480"
     CDS             1940..>2454
                     /locus_tag="VN377_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27868.1"
                     /translation="MSTATSIKNHNSNIEVVVHEKYKDIGYNHEGRRCGEAHSVEREW
                     NQWKPVGKSIFDTILRADVTIGKYHYISTRPPDVAYILNRQEFICQLARDAEKHGVII
                     QTNDKIQTVDDLDGDVLVDASGCPSMVKRELNLELGNIGKTYQETLENANCFCRDTIR
                     IIFSHHGGYFW"
BASE COUNT          662 a          522 c          550 g          720 t
ORIGIN      
        1 ctcgctgatc ggtttttgaa ttcgtaacgc tcgtttccgc gtcaccactg gttctttctc
       61 tttctgaatg accggagctg ctggtggaat ctcaacagat tttgtcggtg ttggagtctg
      121 cacgatttgt ttctgaggtt ccatctgaag tttttgttct tttttctctt ctttttttag
      181 ctcggattcg atttccgctt cgatattctt ttcaaagagt tttaatttct ttttgagaaa
      241 atcaaacatg cttacccttt caacacttct tttatcgttg cgattcttcc atcatctgct
      301 gtgtcttatg cgacagatct tccgcatcgc actgaatctt ctggcccagg gacgccatct
      361 tatcgaggtt ttcttggata cgttttatcc gctcgtcgag cttccccatc gcttcatcgg
      421 ccggtttttc aataacgatt cctgcaccaa cccctatgag gacgtttgat gcgtttttca
      481 gagagccgct gacaaaggtc ccagcaccaa cagggatgag aacctctgag ttgtcatcga
      541 ccgcgctcag ttgttccact gtcattttcg cacgctgata atccatgagg gtcgactgca
      601 ggtattggag ttgcatttca atggattcaa gctgctgttt ataatattca atcaatgtca
      661 ggttcttagt gatttcttct tcttgtgtca taatgtccct tcgattactt ctaatagaaa
      721 tgtgtcacct ttatcaaaga aggtgttatt aatatctttc ttgagcatca ttgggtgcta
      781 taacgcatgc atgcagaggg agaaaaagta aattatttat acaaaccgtc gatttacctt
      841 acgaaggaga aggagagaaa gggacaaacc catgagactc agcagagaat ttactgagaa
      901 attttcagaa atatttttag atgacactat ggctgacttt ttcaataggg aactggatag
      961 ccgctgaccc cctctcatca ttttttttaa ccaagaatcc ccaggcggta acaatatttt
     1021 taatcaacag cctcatttca gagtctgatg aggattcttg ctgtcgctga tatccatgga
     1081 gcacaatatc gcttgaacct tgtgttaaaa aacgttgcct cgtatacccc tgacctggtc
     1141 gtcatctgtg gggatatcac ccagtttggg ccaggggagc ttgcgacgaa ttttctgaac
     1201 caaatccaga tcaaaacatt cgcgattcct gggaacattg atacgtttga tgtcgaccag
     1261 gggattaacg ctagtaatgc gacaaatctt gacaaaagac aggtgattct tcaagatgtt
     1321 cccttcctag ggatcggacg ggagccgcca tcccagcttt caaatcttcc gattgatgat
     1381 ggtacaacaa agaaaccgct gaagaagctg ctgaagacat ccagcatcct ggtgacccat
     1441 tatccaccgt acaaaatcca agacaagata ttcataggaa ctcatggtgg tagcaaagaa
     1501 ctccgcagcc tcattgacac ctacaagcct cgattggttc tctgcggaca catccatgag
     1561 gacccaggga tggccacgat gggtgataca acggtggtga actgcagcat ggggaaacga
     1621 accgaaggag cgctggtaga aatcggtgat acaatcaaag tatcgattct tgactaaaca
     1681 gtggaaaaag gagaacccca gaacggattc aaaccgatgt cacgggaacc atattctttt
     1741 atgattgatt tttacacaac gatgtttgtg gtgttttgag agtgcgtatg atgttcttgt
     1801 gcaaaggcac aggatttttt cattgtttat gtaattttct cctggttcta aaaaaactat
     1861 atatcgtagg aggaaattcc ttgatgcaag gttggtgtga cctatcaagg ttgatataat
     1921 cggtgcaggt cttgggggct tgagtacggc gacctcgata aaaaaccata actcaaacat
     1981 cgaggttgtt gtccatgaaa aatataaaga catcggatac aatcatgagg ggcgacgatg
     2041 tggggaagct cattccgttg aacgtgaatg gaatcaatgg aagccggttg gtaaaagcat
     2101 ttttgatacg atcctgcgag ctgatgtcac gataggaaag tatcattata ttagcactcg
     2161 acctcctgac gttgcttata ttcttaaccg acaggagttc atctgccagc tagcacgcga
     2221 cgcagaaaaa catggggtga tcatccagac gaatgacaaa atacaaacgg ttgatgacct
     2281 tgatggcgac gtgcttgtcg acgcttcagg ttgcccaagt atggtgaaac gagaactgaa
     2341 ccttgaatta ggaaatatcg ggaaaacata ccaagagacc cttgagaatg ccaactgttt
     2401 ttgtcgtgac actattcgga ttatattttc tcatcacggt gggtatttct ggat
//
LOCUS       DATMXJ010000204         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_71444, whole genome shotgun sequence.
ACCESSION   DATMXJ010000204 DATMXJ010000000
VERSION     DATMXJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71444"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VN377_05485"
     CDS             <1..1216
                     /locus_tag="VN377_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27869.1"
                     /translation="QDFQGDAMDFTVQTQPNIGSAQGTDVHNGTYTAPISGMTYGATY
                     RWYVNVTDGTHWTREIFEFQTGFPTNFDPFTFGWQYRKQISINHSQVVGNLTSFPVFI
                     HIIDSDLHEKAQFDGGDILFMEGPGVATKLYHEIERYESSNGELFAWVNIMNLTADEN
                     TVIYMYYGNPDNVNTQMSKRVWDPSFVGVWHLQDFQDSTKNENHGNNYGSMTISGKLG
                     DACRFDGSDDYILVENNPSLNFHTPNRFSVSFWMKRDRLNIYESVISKGTSSTVKGYV
                     VQVWGNNSMYFGVYDGSQEHHLCSNQLITDSNWHYITAVWDGTTQYIFIDGALDNSNN
                     IGSVTIADDSKPLEFGHHYGYMSGQNAYGGSIDEIQISKIDRTAAWIITEYHNQEDPS
                     GFFFVGLEESGP"
     gene            complement(1276..2673)
                     /locus_tag="VN377_05490"
     CDS             complement(1276..2673)
                     /locus_tag="VN377_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27870.1"
                     /translation="MKHFLIIMVLLMILPAWSPATQGTMIFENTSTQKTSQRTNIYDL
                     LIISPKIFHYDLLPLVNHKNRMGIRTVLVDTDEVYDQMFWQGRDQAEKVKYFIKAAIE
                     KWGIHYVLLIGGRSNQGADEKYWVPVRYSSLNRPYEMYPEAHFLSDLYFADIYDKNGS
                     FSSWDDNGNGVFGEWPIGGPAVDHPDLVPDVGLGRLPCLNAQELRIVIRKIILYEAFG
                     CADSWFKTMVVVAGDTYPGRTPYYDGEVYTQQGLDLMTGFRPVKLWASDGSLKNWVDV
                     VSAVNKGCGFLWMSGHGNPKTWATHPPDDNDTWINGLRLRSLPFLHNRQKLPVCITGS
                     GCFNNMFNVSLGNSYFVYGLPTPYCIGWALMIQKDGGSIATIGATAFSYESPDINLGY
                     GGIEWLDRQFFAEYSLNHTDILGDTWSNSITSVLKACPIDWSDGSVDGSAIIVKNVEQ
                     WLLFGDPSLKIGGYR"
BASE COUNT          790 a          625 c          668 g          819 t
ORIGIN      
        1 gcaggatttt cagggtgatg ccatggattt cactgtgcag acccaaccga acatcggctc
       61 tgctcagggg acggatgtcc ataacggcac ctacacggct cctattagtg gaatgaccta
      121 tggagcgaca tatcgttggt acgtgaatgt gactgatggc acacattgga cacgggaaat
      181 attcgaattt cagaccggat ttcctactaa ttttgatccc tttacctttg gatggcagta
      241 ccgaaaacag atttcaatca atcattccca agtagttgga aatcttacct cattcccagt
      301 gttcatccat ataatcgatt ctgatttaca tgaaaaagca cagtttgatg gcggtgatat
      361 attgtttatg gaaggtcctg gtgtcgcgac taagttgtac catgagattg aacggtatga
      421 aagttcgaat ggtgaattat tcgcctgggt taatatcatg aacctcacag ctgatgaaaa
      481 taccgtaatt tacatgtatt atggtaatcc agacaacgtt aatacacaga tgtctaaacg
      541 agtatgggat ccctcttttg taggtgtatg gcatttacag gacttccagg atagtacgaa
      601 aaatgaaaac catggaaata actatggttc gatgaccatc tctggaaaac taggggatgc
      661 ttgcaggttt gatggatctg atgattatat attagtcgag aataatcctt ccctcaattt
      721 ccatactccg aatagatttt ctgtcagctt ttggatgaag agagatcgtt tgaatatcta
      781 tgaaagtgtc atctcgaaag gcacatcatc aactgtaaaa ggctacgtcg ttcaagtttg
      841 gggaaataac tccatgtact ttggtgtcta tgatggttct caagagcatc acttatgttc
      901 gaaccaatta atcacagatt cgaactggca ttatatcaca gccgtttggg atggtacaac
      961 ccagtacatc ttcatcgatg gtgcattaga taattcaaac aatattggct ctgtcaccat
     1021 tgctgatgat tccaaacccc ttgaatttgg gcatcattat ggttatatga gtgggcaaaa
     1081 tgcttacggg ggaagtatcg atgagatcca aatctcaaag atcgatagaa ctgcggcttg
     1141 gatcattact gaatatcata atcaggaaga tccctctgga ttcttttttg ttggtcttga
     1201 ggaatctgga ccgtagacaa catcctgaat cgatcaagtc ctcgtactgt gttatgtggt
     1261 cagaggagat gcggattatc ggtatccacc gattttcagg ctcggatcgc caaagagcag
     1321 ccattgttcg acatttttta ctatgatcgc agatccgtct accgaaccat cagaccaatc
     1381 gatggggcat gcctttaaaa cagaggttat cgagttgctc catgtgtcac cgagaatgtc
     1441 ggtatgattc aggctgtatt ctgcgaagaa ctgccggtcg agccattcga taccaccata
     1501 cccaaggttg atatcagggg attcatagct aaacgctgtc gcaccaatcg tggcgatgct
     1561 gccaccatct ttttggatca tcagtgccca gccgatacaa tacggagtgg gtaaaccgta
     1621 cacaaagtac gaatttccta aactgacatt gaacatgttg ttgaaacaac cgctgcctgt
     1681 gatgcagacc ggtagtttct gacggttgtg gagaaacggc aggctgcgta aacgaagccc
     1741 attaatccag gtgtcgttgt catctggtgg gtgtgtcgcc catgtttttg ggttgccatg
     1801 acccgacatc cagaggaatc cgcatccttt gttgactgcg gatacgacgt cgacccaatt
     1861 cttcaaactc ccatcagacg cccagagttt taccggacgg aaaccggtca tcagatcaag
     1921 gccttgctgc gtatagacct ccccatcgta gtacggggtt cgtcccggat aggtgtctcc
     1981 cgcgacgacg accatggttt tgaaccagga atctgcacac ccgaacgcct catagaggat
     2041 gatttttcgt atgacgatgc gtaactcctg tgcattgaga caaggaagcc ttccaaggcc
     2101 cacgtcagga acaagatccg ggtgatccac tgcaggccca ccaatcggcc attcaccaaa
     2161 cacaccattg ccattgtcgt cccagcttga gaacgaaccg ttcttgtcat agatgtccgc
     2221 gaaatacaaa tcggagagga agtgtgcctc agggtacatt tcatagggtc ggttgagaga
     2281 actatatcgc acagggaccc agtatttctc gtcagcacct tggttgctcc gtccgccgat
     2341 cagcaacaca taatgaattc cccatttttc aatcgctgcc ttgatgaagt atttcacttt
     2401 ctctgcttgg tctctcccct gccagaacat ctggtcgtac acctcgtctg tgtcaacaag
     2461 gactgttctg atgcccattc tattcttatg gttcacgagt gggagaaggt cgtagtggaa
     2521 tatctttggt gatatgatga gcaggtcata gatattggtt cgttgactgg ttttttgggt
     2581 agaagtattt tcaaaaatca tagtgccttg ggtagcgggt gaccatgccg gaaggatcat
     2641 gagaagaacc atgatgataa ggaaatgttt cattttttgg tggtggtcca taatttcctc
     2701 gtattgttaa aatatttgtt atgtgattta aataatttgc tatggtaacg aaatcgtcaa
     2761 tgaaagttaa ggaacagaga aggttttctc cgagaagatc aagggtcgta tcttttaaag
     2821 cattagaggt actggatcaa aaatcaaaaa aattcagaaa atcgatccgc ttggacttca
     2881 cctataccat cctctttatt gt
//
LOCUS       DATMXJ010000205         9180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_50965, whole genome shotgun sequence.
ACCESSION   DATMXJ010000205 DATMXJ010000000
VERSION     DATMXJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50965"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VN377_05495"
     CDS             <1..1070
                     /locus_tag="VN377_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HWR27871.1"
                     /translation="IDCSEGVGRIYDGLLKFHVDEVKLDQLPPTKTQIMMNVGVPEQA
                     FQQGQIPNNGVGLAREEFIINSHIGIHPLALIHYDELKKKASKDKKIAEVIKKIDERS
                     VGYENKVEFFIDTLARGIAKIAAGFHPYDVIVRMSDFKTNEYANLIGGYLYEPEEHNP
                     MIGWRGASRYYDETYKPAFGLECIALKKARNDMGLTNIKPMIPFCRTPEEGRKVIQVM
                     NEYGLKQGENGLEIYVMCEIPSNVIVADQFSEIFDGFSIGSNDLTQLTLGLDRDSDLV
                     AHIFDERNDAVKRLVSQVITVAHNHHPRRKVGICGQAPSDFPEFAEFLVECGIDSISL
                     NPDTVIKTRLKIAETERRLGR"
     gene            1144..1713
                     /locus_tag="VN377_05500"
     CDS             1144..1713
                     /locus_tag="VN377_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27872.1"
                     /translation="MNIVPLVSIKNTKLFDGKEGNSLLPVDVSKRVEKDSLLYVLDLD
                     GIERNNPNLELYQRLAEHNVLWIDNGPRRIDDVMDTIMAGATNITLREESWPTLDMPA
                     VQDLTDDEIYLDKTKQYQEQKILQQNYSGEIGTVLFNEEVLYGVFSKERTANQKIYLY
                     TGSLDKIQLWTEQGISGIIIDLNKKQGGG"
     gene            1713..2201
                     /locus_tag="VN377_05505"
     CDS             1713..2201
                     /locus_tag="VN377_05505"
                     /inference="COORDINATES: protein motif:HMM:NF021513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HWR27873.1"
                     /translation="MDSEAKIILAFLFNRSGKTGLTEAELYLPLSMELGWFSTKEAQQ
                     FVKYAMKKELLVKKDGMLSPSFPVDTIKIPVGFTPSKKTFSETTDDKKGKDLIKEIVS
                     QIFEQTHQDKIEIEEEIKKEGTEKNLLVEVAALYVARKYGVDVSEWYPIVEKSIVKEN
                     KG"
     gene            complement(2182..3522)
                     /locus_tag="VN377_05510"
     CDS             complement(2182..3522)
                     /locus_tag="VN377_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWR27874.1"
                     /translation="MLDLLLIIVFCVLGVLLGVITGLLPGLHVNNVALIMLSTSSAIV
                     TFFSPLYAYGISEQFILILIAGFMISLSISHSFHDTIPTTFIGAPEEETALSVLPAHS
                     LLLKGEGYKAVALAVLGSYGSILVCLALLYPLWFVIGPPLSLYTIMREGMIWVLLAIV
                     VLMISTEKTRITDLGNSGKLPSLLGMLFAAFVFVLSGIFGILIFDFHLTSPIGLPAPV
                     LFPALAGLFGVPTLLNSLFSKPVIPEQKVEPFIQNRIEKKSSLISILTGSFAGMFVSL
                     IPGLTTATGTVLAMSARQKSSQEQTIVTLSAVNTAATFSVTVMLFIILRARSGVTLAV
                     RDLMAIEPWDSFAMPVGLVYLLMFLILSGVLSYFLTLYLGRLFAKKFHVIPYQKLVVF
                     TLIFICALVVLFTGILGLIVLFVATSIGFLPISWGVRRSHCMGILLIPIILYFL"
     gene            complement(3526..4173)
                     /locus_tag="VN377_05515"
     CDS             complement(3526..4173)
                     /locus_tag="VN377_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27875.1"
                     /translation="MKFKYLIILIAVGSLIALYVLSLISQPILVPLSTLQTYNGQQVI
                     VQGVVTDYRTTTYGTQLITIREMQNSTNSVILYIEGEILVEYGDMIQATGEVQQYKNQ
                     WEIVINNPQTLVILQKWHSLSFPLWQLALHPENYLDTTVNVTGIASQTSGSSFTLTST
                     DGKYSIDVMYQSSCPHQFSKGDTVAVGARFLYDPTTCRFFLKATEETHGIWKIGR"
     gene            4322..6892
                     /locus_tag="VN377_05520"
     CDS             4322..6892
                     /locus_tag="VN377_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HWR27876.1"
                     /translation="MVREVVLKVSEAFQQDVGYGRARIDHQTRMDLDLSIGDVIELEG
                     TKVTATSVWRAHPSDEGKRLVRIDNLTRKNAGTGLGDRVKIRRAEIKEARDVALAPLM
                     PEGQRIEFGMGIDVIIKKNMLRRPITKGDEIIIPGIAFFGNALPFVIVDTNPHGIVMI
                     TERTVLHVKEEAAKITEEEGPRVSYEDIGGLHLEIQKVREMIELPLKHPELFDRLGID
                     PPKGVLLHGPPGTGKTLIAKAVANESGANFYTINGPEIMSKFYGQSEENLRKVFEEAQ
                     KNAPSIVFLDEIDAIAPKRSEVHGEVERRVVSQLLTLMDGLKGRGKVIVIGATNIPDV
                     IDPALRRPGRFDREIRIDVPDRNGRKEILLIHTRGMPINPGLGEMYSGETISEEKILN
                     AIKQGIKKIHISDLLRSMLRSKDKNKRMEVVGKYLDVVFASFKDQLPPLFYEKVIRLI
                     ADEITNLLPYDLDDSEANVSAEQQISERLMTIRENPDEFIKRISKEALLDEIADITYG
                     FVGADLAALAREAAMNALRRYLPEIDLEKPIPIELLEKMEVTLDDFKNAHRGIEPSAL
                     REFFVEIPKISWEDIGGLEDIKQSLKEAVEWPLAQPEVFKRMGIHAPRGILLYGPPGT
                     GKTLLAKAVAHESKANFISIKGPEVLSKWVGESEKAVRELFKKARQVAPTIVFLDEID
                     SIAPRRGTFEGSHVTESVVNQLLTSIDGMESMEGVVVIGATNRPDIIDPGLLRPGRFD
                     RLLLIPAPDSVSRLEIFKIHTKDMPLAKDVSLDDIAEKTVGFSGADIDALCREAAMTA
                     LRDDINAKEVRKAHFDRAMVEARGSLTDDVIKYYKKVKDDMGSSIAKKDKKERDIQYM
                     "
     gene            6920..7159
                     /locus_tag="VN377_05525"
     CDS             6920..7159
                     /locus_tag="VN377_05525"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWR27877.1"
                     /translation="MKVLESDLRGKTVMSEEGLYLGILRNSLVDERTGQLLSILVEPS
                     EDIDPRLYHLDEVGHLVFPFEAIKSVKDVIIVGGN"
     gene            7538..8803
                     /locus_tag="VN377_05530"
     CDS             7538..8803
                     /locus_tag="VN377_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HWR27878.1"
                     /translation="MPKETFNRDIFSHLLSSEGLFTDREVMRSSYTPEILPHREKEIN
                     DLANILVPALKGESPSNVFIYGKTGTGKTAVVKFVGKELLKKGEQTGKKVNFIYMNCE
                     IVDTQYRLLQNIANRFINEWNDHVPFTGWPTDEVYSKVKGMMDKEKSVTVIVLDEIDK
                     LKGDEALYNLTRINTELDKARVSVIGISNDLKFTDFLDPRVKSSLGEESMIFYPYDAE
                     QLQDILRARIKTGLKQGVIEEDVVPLCAALSAQEHGDARRALDLLRVSAELAERGKVP
                     KITRSHVRLAQNKIELDRITEVVRTLPNQSKLVLYSIFILDKQNKKAGNTQALTTGEV
                     YEVYRELCKKTRYNCLTQRRTADLISELDMLGVITARVISKGRHGRTTEIQMSTSSSS
                     KDLPTVLQEDDMFKELGNYKMKGQARLTV"
     gene            8843..>9180
                     /locus_tag="VN377_05535"
     CDS             8843..>9180
                     /locus_tag="VN377_05535"
                     /inference="COORDINATES: protein motif:HMM:NF044675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HWR27879.1"
                     /translation="MHTVLHPSASDALHFIREHHSSKVEKTMLLVMGECMVDYHGRAK
                     SLLDWGERLIMIKQDGTVLVHQPVMREPVNWQPTGSITEFKTKDDQLILNCRHLKPPE
                     KMKVVFRQLKL"
BASE COUNT         2685 a         1876 c         2356 g         2263 t
ORIGIN      
        1 cgatagactg ttccgaagga gttgggagaa tctatgacgg gttgctgaag ttccatgtcg
       61 atgaagtaaa actggaccag ctcccaccta ccaagacgca gatcatgatg aacgtcggag
      121 taccagaaca ggcgttccaa caaggacaaa tcccgaacaa tggtgttggt cttgctcgag
      181 aggaattcat cattaactcc cacatcggta ttcatccttt ggcattaatt cattatgatg
      241 agttgaagaa gaaagcctca aaggacaaaa aaatcgctga ggtcatcaaa aaaatcgatg
      301 aacgcagcgt cggctatgag aacaaagtcg agttctttat cgatacgtta gcgcgtggga
      361 ttgcgaagat agcagctgga ttccatccgt atgatgtgat cgttcggatg tctgatttta
      421 agacaaatga gtacgcgaac ctgattggcg ggtatctgta cgagcctgag gagcataacc
      481 cgatgatcgg ctggcgagga gcttcgcggt actacgacga gacatacaaa cctgccttcg
      541 gccttgaatg tatcgcgttg aaaaaagcac gcaatgacat ggggctgacc aacatcaaac
      601 ccatgatccc gttctgccgc acacctgaag aaggaagaaa ggtcatacag gtcatgaacg
      661 aatatggttt gaaacaagga gaaaatggtc tggaaatcta cgtcatgtgt gaaatccctt
      721 ccaacgtcat tgtggctgac caattctcag agatcttcga tggttttagc atcgggtcga
      781 acgatctgac gcagctcacc cttggtttgg accgtgactc ggaccttgtg gcacatatct
      841 tcgatgagcg gaacgatgca gtcaaacgac tcgtttcaca agtcatcacc gttgcgcaca
      901 atcatcatcc gcgcagaaaa gtcggtatct gcgggcaagc accctctgat ttccctgagt
      961 ttgccgaatt cctcgtggag tgcggcatcg acagtatctc tctgaacccg gacacggtca
     1021 tcaagacacg attgaagatt gctgagacgg agcgacggct cgggagataa ttctcttctt
     1081 tttttctttt aatgaaagta atgatctgaa aagtggtgtg gaaaatacct ggttgtgtga
     1141 ccaatgaaca ttgttccgct tgtttctata aaaaatacca aactgtttga tgggaaggaa
     1201 gggaatagtc ttttacccgt agatgtgtcg aaacgagttg aaaaagattc cttgttgtac
     1261 gtgctagatc tagatggcat cgaacgtaat aacccgaatc tggaactgta tcagaggcta
     1321 gcagaacaca acgtcctttg gatcgataat gggcctcggc ggatcgatga cgtgatggat
     1381 acgattatgg ctggtgccac aaatatcaca ttgcgggagg agtcctggcc gactcttgac
     1441 atgcctgctg tgcaggacct gaccgatgac gaaatctatc ttgataagac caaacaatac
     1501 caagagcaaa agatcctgca gcagaactac tcaggagaga tcgggacggt tctatttaat
     1561 gaagaggtac tgtacggggt tttttcaaaa gagaggactg caaatcagaa aatatatctc
     1621 tatacaggtt ctcttgataa aattcaactc tggacagaac aaggcatttc cggtatcatc
     1681 atcgatctga ataaaaaaca aggtggaggg taatggactc tgaggcaaaa atcattctcg
     1741 cgtttctttt caaccggagt ggaaaaaccg gtttaacaga ggcagaatta tacttgccgc
     1801 tctccatgga gttaggctgg ttctccacaa aggaagcaca gcagttcgtg aaatatgcaa
     1861 tgaaaaaaga actcttggtg aaaaaagatg ggatgttgtc tccaagtttc ccggttgata
     1921 caatcaagat ccctgtcggg ttcacccctt cgaaaaaaac ttttagcgaa acaacagatg
     1981 ataaaaaagg aaaagacctc atcaaggaaa ttgtctcgca gatttttgaa caaacccatc
     2041 aggataaaat cgagatagaa gaggaaatta aaaaagaagg aacagaaaaa aatctgctgg
     2101 ttgaagtcgc ggcattgtat gtcgcaagaa aatacggggt ggatgtttct gagtggtacc
     2161 ccattgttga aaagtctatt gttaaagaaa ataaaggata atcgggatca atagaatccc
     2221 catgcagtgg ctccgtcgga caccccagct gatcggtaag aacccgatgc ttgtcgcaac
     2281 gaatagcacg atcaatccaa ggattcctgt gaagagaaca acaagtgcac aaatgaaaat
     2341 aagggtaaag accacgagct tttgataggg gatgacatgg aatttttttg caaacagcct
     2401 tcccagatac aatgtgagaa aatacgaaag aaccccgctt aaaataagga acattaacag
     2461 ataaaccaat cccacaggca tggcaaacga gtcccagggc tcaatcgcca tcagatccct
     2521 cactgcaagc gtgacaccgc tacgtgcccg tagaataata aacaacatga cagtgactga
     2581 gaaggtcgcc gcagtgttca cagcggaaag ggtaacgatg gtttgttctt gacttgattt
     2641 ttgtcgtgcg ctcatcgcaa gcacagtacc tgttgccgtg gtcaaaccag gaatgagtga
     2701 gacgaacatc ccagcaaaac taccggtgag tatggaaata agcgaggatt ttttctcgat
     2761 tcgattctga ataaagggtt ctaccttctg ttctggtatg acgggcttgg aaaataatga
     2821 gttaagaagg gtcggtactc cgaataatcc tgcgagtgct ggaaacaaca cgggggcggg
     2881 cagcccgatc ggtgatgtaa ggtgaaaatc aaaaataagg atgccgaaaa ttccagagag
     2941 aacaaagaca aaagcagcaa acaacatccc gaggagagaa ggtagttttc ccgagttacc
     3001 aaggtctgtg atccgtgttt tttctgtaga aatcatgagg acgacgatcg cgagaagcac
     3061 ccatatcata ccctctcgca tgatggtgta caatgatagt ggaggtccaa tgacgaacca
     3121 aagaggatag agaagcgcaa gacagacaag aatcgaaccg taactcccca gaactgcaag
     3181 tgcaactgcc ttgtatcctt ctcctttaag caaaaggctg tgagcaggga ggacggacag
     3241 agcggtttct tcctcaggtg ctccgataaa agtggtaggg atcgtgtcat ggaatgagtg
     3301 tgaaatggaa agagaaatca tgaatcccgc tattaaaatc aggatgaact gttccgagat
     3361 gccataggca tataatggac tgaaaaaagt aacaatggcg ctggaggttg acagcatgat
     3421 caatgcgaca ttgttcacat gcaagccagg taacagacca gtgataacgc caaggagtac
     3481 gcctaggaca caaaaaacga tgattaagag cagatcaagc attcgttacc tccctatctt
     3541 ccaaatgcca tgtgtttctt cggttgcttt gaggaaaaat ctgcatgttg ttggatcata
     3601 tagaaagcgt gctcccactg ccacggtatc accttttgag aattgatggg ggcaggagga
     3661 ttgatacatg acatcaatgg aatattttcc atccgtgctt gtgagcgtaa atgaggatcc
     3721 ggatgtttgt gaggctattc cggtgacgtt gactgtggtg tcaagatagt tttctgggtg
     3781 aagtgcgagt tgccagagag gaaacgacaa ggagtgccat ttttgaagaa tgacgagcgt
     3841 ttgtgggtta ttgatgacga tttcccattg gtttttgtat tgttgtactt cgccagtcgc
     3901 ctgtatcatg tcaccatatt caacaagtat ctctccttcg atatacagga tcactgaatt
     3961 tgtgctgttc tgcatctctc tgatggtgat gagctgagtt ccgtatgtgg ttgttcggta
     4021 gtcggtgaca acgccttgga cgatcacttg ttgtccatta taggtctgta gtgttgataa
     4081 aggaacaaga atgggttgtg agataagtga taaaacatat agggcaatca acgatccgac
     4141 tgcgatgaga ataataagat atttgaattt catgtgtttc ccccagactg taattataaa
     4201 agagaaaata tagaatcttt tattaaatgt tccgttgtgt gcttctccct tagcggcagg
     4261 ggagaaaact ttataacgcg cgttaatctt cctgccacaa taattacgac tggtgggagt
     4321 tatggtacga gaagttgttc tgaaagtgtc agaggcgttc caacaagatg ttgggtatgg
     4381 gagagctcgg attgatcatc agacacgaat ggatcttgat ctttctatag gggatgtcat
     4441 tgagcttgaa gggacgaagg tgacagcgac gtctgtatgg cgagcgcatc cttcggatga
     4501 gggaaaacgt cttgtacgta ttgataacct gaccaggaaa aacgcgggga ccggactggg
     4561 ggatcgggtg aagatcagac gtgctgagat aaaagaagca cgcgatgtcg cacttgctcc
     4621 gttgatgccg gaaggccaac gtattgagtt tggtatgggg attgacgtta tcataaagaa
     4681 aaatatgcta cgtcgaccaa tcacgaaagg tgacgagatt ataatcccgg ggatagcgtt
     4741 ttttggtaat gcactccctt ttgtcattgt ggataccaac ccgcatggga tcgtgatgat
     4801 cacagaacgg actgttcttc atgtcaaaga agaagcagcg aaaatcactg aggaggaagg
     4861 cccacgtgtc agttatgaag acatcggtgg cctccatctt gagatccaaa aagtacgaga
     4921 gatgattgag ctaccgttga agcatcctga actgtttgac cggttaggga ttgatcctcc
     4981 gaagggagtt cttctgcacg gtccaccagg gacggggaaa acattgattg ccaaagcggt
     5041 cgccaatgag tcaggggcga atttctacac gatcaatggt ccagagatta tgtcaaagtt
     5101 ctatgggcaa agtgaagaga acctcaggaa agtctttgaa gaagcccaga agaacgcacc
     5161 ttccattgtg ttccttgacg agattgacgc gatcgcacct aaacgcagcg aggtgcatgg
     5221 tgaggtggaa cgtcgtgttg tttctcagct gctgacattg atggatggtt tgaaggggcg
     5281 gggtaaagtc atcgtgattg gtgcgacgaa tatccctgat gtgattgacc ctgcgttgcg
     5341 acgaccaggt cggtttgatc gtgagattcg catcgatgtc ccagatagaa acggaaggaa
     5401 agagatcttg ttgattcata ctcggggtat gccgattaac cctggtcttg gtgagatgta
     5461 ttccggggag acgatcagtg aggagaaaat cttaaacgcc atcaaacagg ggatcaagaa
     5521 aatccatatc tccgatctgt tgcgaagcat gttacgttcg aaggataaaa ataaacgtat
     5581 ggaagtggtt ggtaaatatc ttgatgtggt gtttgcgtca ttcaaagatc aacttccacc
     5641 tttgttttat gagaaggtca ttcgattgat tgctgatgag ataacgaatt tattgcctta
     5701 tgatttggat gattctgaag cgaatgtcag tgcagagcag caaattagcg agcgattgat
     5761 gacgattcgg gagaatcctg atgagttcat caaacggatt tcaaaggaag ctcttctcga
     5821 tgagattgcg gatatcactt atgggtttgt aggggcagac cttgctgcgt tagcacgaga
     5881 ggctgcgatg aacgcgttgc gacgatacct ccctgagatc gatttggaaa aaccgattcc
     5941 gattgagctt ctggagaaga tggaagtgac gttggatgat tttaagaacg cacatcgggg
     6001 tattgagcca tctgcattac gtgagttctt tgtagagatc ccgaagattt cttgggaaga
     6061 catcggtggt ttggaggata tcaagcaaag tctgaaggaa gcggtggaat ggcctttggc
     6121 tcaaccggag gtgttcaaac ggatggggat acatgcgcct cgcggcattt tactgtatgg
     6181 cccccctgga acaggaaaga cgctgcttgc taaggcagtc gctcatgaat caaaggcgaa
     6241 tttcatctcc attaaagggc cagaggtgct cagtaaatgg gtcggagaaa gcgaaaaagc
     6301 ggttcgtgag ctgttcaaga aggctcgtca ggtcgcacca acgatcgtgt tccttgacga
     6361 gattgattct atcgcaccac gtcgagggac gtttgaaggg tcgcatgtga ccgagagtgt
     6421 ggtgaatcag ttgctgacga gcatcgacgg gatggaaagc atggagggtg tggtggtgat
     6481 cggggcgacg aaccggccgg atatcattga tcctggtttg ttgcggccgg gtcggtttga
     6541 ccgtttattg ttgattcccg cgccggatag tgtgtcgcgg cttgagatct tcaagattca
     6601 tacgaaagat atgccacttg cgaaggatgt gtctcttgat gatatagcgg agaagactgt
     6661 cggattttct ggtgcagata tcgatgcgct ctgtcgtgag gctgcgatga ctgcgttgcg
     6721 tgatgatatc aatgccaagg aagtccgtaa ggcacatttt gaccgtgcga tggttgaggc
     6781 tcggggaagt ctgactgatg atgtgataaa atattataaa aaagtaaaag atgatatggg
     6841 tagtagtatc gccaaaaagg ataaaaaaga acgagacatt caatatatgt aagttagtat
     6901 aaaaaacaag agggatatga tgaaagtact ggagagtgat ttgagaggaa aaacagttat
     6961 gagtgaagag ggcttgtacc tcggcattct gaggaattcg ctggtggacg agagaactgg
     7021 tcagttgctc agtattcttg tggaaccttc tgaggatatt gatccacggc tgtatcatct
     7081 tgatgaggtt ggccatttgg tgttcccttt cgaagccatc aaatcagtaa aagatgttat
     7141 tatcgttggt ggtaactaag aggcaaccct cttatttttc cttctttttt attccctaag
     7201 cgtacagttt tttccaggag aaaagttttg tgattattgt tttgggggga cccctttgtt
     7261 tcgtatggaa acctgtggga gatgtagtcg cttgttgaga agaatgttga cgtcagtagt
     7321 cttggatgta tatgtatttc gaactatttt aatattgcca tttataaaaa tatattatta
     7381 aaaaaatata aagattaaaa caatgcgtgc tacctgtttc catgcgaaat catcccctcc
     7441 cccgttctct aatgtaacag gagaaaaact tgacaaaaat tgacacaaaa atttattata
     7501 cagtaaaccc ttacagccct acgaggaggc gtgttttatt cccaaagaaa cattcaacag
     7561 agacatcttc agccatctac tcagcagtga agggctgttc accgacagag aagtcatgcg
     7621 gtcatcctat acaccagaga tccttccaca ccgagaaaag gaaatcaacg acctagccaa
     7681 tattttggtc ccagcactca agggagaatc accgtccaat gtgtttatct acggaaaaac
     7741 cgggacaggg aaaaccgcgg tcgtcaaatt cgttggaaaa gaactactaa aaaaaggcga
     7801 acagaccggc aagaaagtca atttcatcta catgaactgc gagattgtcg acacacaata
     7861 ccgtcttctg cagaatattg cgaacagatt catcaacgaa tggaacgacc atgtcccctt
     7921 caccggatgg cccaccgatg aggtatactc aaaggtaaaa gggatgatgg acaaagaaaa
     7981 aagcgtcacg gtcatcgttc tggatgagat cgataagctc aaaggtgacg aagccttgta
     8041 caacctcacc cgtatcaaca ctgaactgga caaagcccgt gtgtctgtta tcggtatatc
     8101 gaatgatttg aaattcaccg atttcctcga ccctcgcgtc aaatcaagcc ttggcgagga
     8161 aagcatgatt ttctatccgt atgacgcgga gcaactccag gatatcttac gagctcggat
     8221 taaaacaggc cttaagcaag gagtcatcga agaagatgtc gtccccttgt gtgctgcctt
     8281 atccgcccag gagcacggag acgcccgccg agcgcttgat ctcctcaggg tgtctgccga
     8341 gcttgcagaa cgaggtaagg tcccgaaaat caccagatct cacgtgcgtc tcgcgcagaa
     8401 caagattgag ctggaccgta tcaccgaggt cgtccgcacc cttccgaatc aatccaagct
     8461 cgtgctctat tcgattttta tcctcgataa acaaaacaaa aaagcaggga atacccaggc
     8521 actgacgacc ggtgaggtct acgaggtgta cagagaactc tgtaaaaaaa ccaggtacaa
     8581 ttgcttgacg caacgaagaa ccgctgatct catctccgag ctggatatgc ttggagtcat
     8641 cacagcccgc gtgatttcga aaggccgtca tggccgcacg acagagatcc agatgtccac
     8701 ctcatcatcc tcaaaagatc ttcccaccgt ccttcaggag gacgacatgt tcaaggaact
     8761 gggcaactac aagatgaaag gtcaagctcg tctcactgtc taacgtggtg cttaccattc
     8821 atataccgta aggagttagg ttatgcatac ggttcttcac ccctccgcat ccgatgcact
     8881 gcattttatc agggagcatc attcctccaa agtagagaaa accatgctgt tggtgatggg
     8941 agaatgcatg gtggattatc acgggcgcgc aaaatcgtta ctggactggg gtgagcgtct
     9001 catcatgatc aagcaggatg ggaccgtgct ggtccatcag ccagtgatgc gtgagccggt
     9061 gaactggcag ccgaccggca gcatcaccga gttcaaaaca aaggacgatc agctcattct
     9121 caactgccgg catctcaaac cccctgaaaa aatgaaagtg gtcttccggc aactcaagct
//
LOCUS       DATMXJ010000206         8766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_19899, whole genome shotgun sequence.
ACCESSION   DATMXJ010000206 DATMXJ010000000
VERSION     DATMXJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19899"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VN377_05540"
     CDS             <1..430
                     /locus_tag="VN377_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27880.1"
                     /translation="IENNRITTCFEYCIYLYNHSTNNIIVNNSLPGLSTNGISLYGGC
                     NNNRIEKNSIEDKVNGVIIEWSFFNMIRNNNFINNTKQAYFENSSLNLFIKNYWDDWM
                     VKKPRPIEGIRYGYFLQNHRAWTIYDLRPTQEPYDIPGLR"
     gene            430..891
                     /locus_tag="VN377_05545"
     CDS             430..891
                     /locus_tag="VN377_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27881.1"
                     /translation="MDKKPLIVVSICAVVVLVLGSSVPALAKTQSFVSKTIPLISTSS
                     AEDYKVYIGAGFIRQKGDWKFGLGWHMTIIDTGDTNVTGFFYGNETTLFGKVLPGNTG
                     PFSLASGEMIVASSSFYYLHPLVFVHLTVKIGNMTYSKSGYEVGPFTFLVG"
     gene            897..1538
                     /locus_tag="VN377_05550"
     CDS             897..1538
                     /locus_tag="VN377_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27882.1"
                     /translation="MKKLILVGISLCAVVLLVLGSLSNVVGYQSVQSSNQQAIKEEVN
                     QRELLFQTIVDIANNKEIQRIILKSQMGRGIFPVSDIPILTKGQLKVMFLVGLMLSNV
                     ISKSRMQSMIGEYQFSSQEMQKGLSAAIEKDATLNGEMTQLSNSQCDCENRNTTRWSF
                     PVLCSIFPSMNFLGIILLNILYLGIILKIIFTVIAVIIYILMMVFHYLLSFLP"
     gene            1603..2853
                     /locus_tag="VN377_05555"
     CDS             1603..2853
                     /locus_tag="VN377_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27883.1"
                     /translation="MKRKWLAIGIILLFLGTCLIPATAQDTEKSQSTSRGNWLYVGGS
                     GPGNYTKIQDAIDNTTDGDTVYVFPGIYKERIRINNSIQLQGVDPITTIIDGQNTSND
                     ILTCVGTDAVISGFTIFNCSMSHSCILLNHTVNCTLYGNIIHSGGYGVTVQNAQNISI
                     INNTFLQILTTRTGYIAITLNNCIFSTCSQNKISSWDGGILIKGTHLLITQNTITNTH
                     RGITDMMNSLPFTNKYFIIDENHLNNNEVAIFLAGSRDYSITRNEITNSTTVGLYMVE
                     EVFTSVDPENITIEDNLITYSVQGMVVENSININIEGNHIQHNTLGLSFLYSSFTSVK
                     RNTFQDNTETVVYQWAFFPFSRFNYKVPQFDMNFWDQPRGLPYPIAGRWGFFKPYFFF
                     SPINIFPWMTFDWHPAQEPYDIGV"
     gene            2857..3882
                     /locus_tag="VN377_05560"
     CDS             2857..3882
                     /locus_tag="VN377_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWR27884.1"
                     /translation="MKRKALAIGIILLFLGTCIIPAIAQDTEKSQQASRGNWLYVGGS
                     GPGNYTKIQDAIDNASDGDTVFVLNGIYYENVRVDKSIALLGENQTGTIIDGQEQNGH
                     LISIIAAGVTISGFTIRNCGGTPNAAEIYVNSDNNKIIGNTFICTTLHSEEGIWVWHS
                     SGTTISGNTITSHLYGIWLENCTNNNITHNYISTVSEWGIILGNSEGNSIYENSVTDN
                     RGGIYLRDANRNMLFGNNITKNTRGVALVESVSTSSNNTILKNNFKKNRGFDASFILA
                     KKSHAKNLWEGNYWNRPLRFPKLILGGKEFFSIPGIPFHFPGMIISLPWFNIDWHPAQ
                     EPFDIGV"
     gene            3889..4743
                     /locus_tag="VN377_05565"
     CDS             3889..4743
                     /locus_tag="VN377_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27885.1"
                     /translation="MKDTYVKKGLVVSVILLFIGVAVAPFINQSVVKASSDDDLVEIT
                     TQACGIQGYGNNTVKLTREQYQDLEAYLVEFRARLNQTSTREDAVPIFKEAVVELDKY
                     GLLPRGMSVETAQRLILKKYKMMDEFGSRNKSLNINLGCLVAGKVDFEIGFYGRLFLL
                     LSRIWFLTFPLMLMLYILSLVNPVSLGGVITFGSHTIYQHGSQYLPAVGWVTALGILG
                     QQHWNHSFYGKISPIQILVKGGDQIVYYYEFIGAIGFCGIKIVPFGFFIGSALAVSLD
                     SEKPIEVN"
     gene            5082..5741
                     /locus_tag="VN377_05570"
     CDS             5082..5741
                     /locus_tag="VN377_05570"
                     /inference="COORDINATES: protein motif:HMM:NF003276.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HWR27886.1"
                     /translation="MQPTENLGIYKKNDRLFTVNPENCRGTKVYNEQLFTEDDKEYRS
                     WNPFRSKLAAALLKGITLDLSPTTSILYLGAATGTTVSHLSDIVTKGTLYAVESSPIA
                     IISLLKVAEKRQNIIPILSDANHPERYQHLVPTVDLVYQDISQRNQADIFTRNIRAYL
                     KPNGTGLIMVKARSIDVALKPKQAYELVCSALQKEKFRMKKTVDLTPFEKDHAAIIMN
                     L"
     gene            complement(5748..6722)
                     /locus_tag="VN377_05575"
     CDS             complement(5748..6722)
                     /locus_tag="VN377_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWR27887.1"
                     /translation="MVETMPDQSLALEVFLKHTHEDIEKKINELITDQNILTILKEGK
                     RLRPLLGALSFKVCTGGNESSQLYQQFLEGTVAIELAHGASLVHDDIIDGDLTRRGEP
                     AFYIKTGIDNAILIGHKMLVMGFNLALSHGDRIARLYVDAWGGTLDGQLSEINFNSKD
                     LEDVNITADSKFFQVYSKIIDLKTATLFSAACKAAAYWAKSSEEIANVLAEYGREVGF
                     AYQLADDLVDLEKGEMIDSVVIPLLTRLEKRTAKNGNLKHRAIRKKIEKKSPEIKKLY
                     LEEISKHVNKAQKICNSPLLPDNQFKTFLLDAPYYSINKMLKEIYINI"
     gene            complement(6778..7407)
                     /locus_tag="VN377_05580"
     CDS             complement(6778..7407)
                     /locus_tag="VN377_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWR27888.1"
                     /translation="MTRWYKEKKKEHFYKEAKRVGYRARSAFKLQQIQNRFHLIPKNG
                     VIVDLGAAPGGWSQVAKELVGTDGIVIGVDLSSIKPFEGIQFLQGDVTKTETLEQIKS
                     LMNGKNADVILSDMSPDISGNYSIDQARSAWLCECALLAVTQILQPGGHFICKIFEGE
                     DTKAFIEKVKLQFMVVKTFSPEASRKSSSEVYIIAKSFKNPCKKYHDSS"
     gene            complement(7414..8361)
                     /locus_tag="VN377_05585"
     CDS             complement(7414..8361)
                     /locus_tag="VN377_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HWR27889.1"
                     /translation="MQSKKKKEKRQSIEEIIQCPECNSTHLSKDYARAELVCADCGLV
                     IDQDFIDHGPEWRAFDSDQREKRARVGAPMTYTIHDKGLSTMIGWKNRDSYGKSIPTR
                     NRAQLYRLRKWQRRIRISDATERNLAFALSELDRMASGMGLPRTVRETAAMVYRKAVL
                     KNLIRGRSIEGVSASALYAACRQCNVPRTLDEIAASSRVSRKEIGRTYRFIARELGLK
                     LMPTSPQDYISRFCSELKLSGDVQAKAIEILKDAADKELTSGRGPTGVAAASIYISSI
                     LCGERRTQREVADVAGVTEVTIRNRYKELAERLDIDIIL"
BASE COUNT         2551 a         1750 c         1824 g         2641 t
ORIGIN      
        1 tatagaaaat aatcgtataa ctacctgctt tgaatactgt atttatttgt ataatcattc
       61 gaccaataac ataattgtaa ataattcact tccgggtcta tcaacaaatg ggattagtct
      121 ttatggaggc tgtaataata atcgtattga aaaaaactcg attgaagata aagtaaatgg
      181 tgttattatt gaatggtctt tttttaatat gattcgaaat aataatttta ttaataacac
      241 aaagcaagca tattttgaga atagttcatt gaatctcttt attaagaatt actgggatga
      301 ttggatggtt aaaaaaccac ggcccattga gggaattcga tatggctatt ttctgcaaaa
      361 tcaccgtgca tggacgattt atgatttgcg tcctacacaa gaaccatatg atatccctgg
      421 gctgagataa tggataagaa acctctgata gttgtaagta tctgtgctgt ggttgttctt
      481 gtgttgggtt caagcgttcc tgccttagct aaaacccaat cttttgtatc taagacaata
      541 cccttaattt ctacttcatc agctgaggac tataaagtgt acatcggagc agggtttatt
      601 cgtcaaaaag gtgattggaa atttggcttg ggttggcata tgacaattat tgatactggt
      661 gacacgaatg tcactggttt tttttatggg aatgaaacta cattatttgg gaaagtcctt
      721 cccggcaata caggaccatt ctcattggca tctggcgaga tgattgttgc tagtagttcg
      781 ttctattatt tacatccgct agtttttgtt cacctcactg ttaagattgg gaacatgacc
      841 tattcaaaat ctggatatga ggtaggtcct ttcacattcc tagttggttg aaaataatga
      901 agaagttgat attagtagga atcagtctct gtgctgtggt tcttcttgtt cttggttcgt
      961 tgagtaatgt ggtgggatat caatcggttc aatcatcgaa ccaacaagcc ataaaagaag
     1021 aagtcaatca gagagagttg ttattccaga ccatcgttga tattgcaaac aacaaggaga
     1081 tacagcgaat catcctcaaa tcccagatgg gtagagggat attcccagta tcagatattc
     1141 ctattttaac gaagggtcaa ttgaaagtga tgtttctggt tggtttgatg ctttcaaatg
     1201 ttatcagtaa atcaagaatg caatctatga ttggtgagta tcaatttagt agtcaagaga
     1261 tgcagaaggg attatctgct gctattgaaa aggatgcaac actaaatgga gaaatgacac
     1321 aattatcgaa ctcacagtgt gattgtgaaa acagaaatac aactcgttgg agttttcctg
     1381 ttctctgttc aatctttcct agtatgaact ttttaggtat aattctttta aacatcttat
     1441 atttaggaat aatattaaaa ataatattca cagtaatagc agtaataata tatattctaa
     1501 tgatggtttt ccattaccta ttatcgttct taccgtgatt aataatatta aattaatgac
     1561 agacaacaac cattagaaac cgatgattta ggagaataat caatgaaaag gaaatggtta
     1621 gcgattggaa tcattctctt attcctgggg acctgtctca ttccagcgac tgcacaagat
     1681 acagagaaat ctcaatcgac atcaagagga aattggctgt atgtcggtgg aagtggacca
     1741 gggaactata cgaagattca ggatgcgatc gataacacaa cagatggaga caccgtttat
     1801 gttttcccag gtatatacaa ggaacgaatc agaataaaca acagcatcca gctccagggt
     1861 gtagatccca ttaccaccat cattgatggc cagaatacca gtaatgatat cctaacttgt
     1921 gtcggaacag atgcggtcat tagtggtttt acaatattca attgttccat gagccactcc
     1981 tgtatccttc ttaaccacac cgtgaactgt accctttatg gaaatattat ccattcagga
     2041 ggatatggag tcaccgtcca aaatgcacag aacatcagca tcatcaataa cacatttctt
     2101 caaatcctaa cgacaagaac cggatatatt gccattactc tcaataattg cattttcagc
     2161 acttgttcac agaataaaat ctcatcatgg gatggaggta ttcttatcaa gggaacccac
     2221 cttctcatta cccaaaacac cattacgaat actcatcgtg gtatcactga tatgatgaat
     2281 tctcttcctt ttacaaacaa gtatttcatc atcgatgaga atcatttgaa taataacgag
     2341 gtagcaatct ttctcgctgg ctcaagggat tattcaataa cacgaaatga aattacaaat
     2401 tccacaacgg tcggacttta catggtagag gaggttttta cgagtgtcga tccagaaaac
     2461 attaccatcg aagacaatct cattacgtac tcagtccagg ggatggttgt tgagaattcc
     2521 atcaacataa atattgaagg aaaccatatt caacacaata cccttggtct ttcatttctc
     2581 tatagctcct ttacgtcagt gaagaggaac accttccaag acaataccga aacagtcgtc
     2641 tatcaatggg cgttctttcc attctcacgc tttaattata aagtaccgca atttgacatg
     2701 aatttctggg accaaccacg gggactaccc tatcctattg ctggtcgatg gggtttcttc
     2761 aaaccatatt tctttttcag tccaatcaat atttttccct ggatgacctt tgactggcac
     2821 cctgctcaag aaccctatga tattggagta taatcaatga aaaggaaagc tttggcgatt
     2881 ggaatcattc tcttgttcct gggaacctgt atcattccag cgattgcaca agatactgag
     2941 aaatctcaac aagcatcaag agggaattgg ttgtatgttg gcggcagcgg acctgggaac
     3001 tatacaaaga ttcaggatgc gattgacaat gccagtgatg gtgatacggt atttgtttta
     3061 aatggtatat actacgaaaa tgtaagagtg gacaaatcca ttgctctttt aggggaaaac
     3121 cagaccggta ccattatcga tggtcaagaa caaaacggtc atcttatcag catcatcgca
     3181 gcaggagtaa ccatcagtgg gttcaccata cggaattgcg gtggaacccc gaacgcagca
     3241 gaaatctacg tgaattctga caataacaaa atcatcggta ataccttcat ctgcaccaca
     3301 ctacacagcg aggagggtat ctgggtatgg cactcttcgg ggactaccat ctcaggaaat
     3361 acgatcacaa gccatctgta tggtatctgg cttgaaaatt gtacgaacaa caatattact
     3421 cacaactata tttctactgt tagtgaatgg gggatcatcc ttggaaattc ggagggaaac
     3481 agcatctatg aaaatagtgt gacggacaat agaggtggca tctaccttcg cgatgcaaat
     3541 agaaatatgc tctttggaaa caacatcaca aaaaacacaa gaggggtggc actcgtcgaa
     3601 agtgtttcta cctcatccaa taatacaata ctgaaaaaca atttcaagaa aaacaggggt
     3661 ttcgatgcat ctttcatcct ggcaaaaaag tcacatgcta aaaatctatg ggaggggaac
     3721 tattggaaca gaccgttacg gttccctaaa ctgattcttg gtggcaaaga attcttttcc
     3781 attccaggaa ttccattcca ctttcccgga atgataattt ctctgccatg gttcaacatc
     3841 gattggcatc cagcacaaga accctttgat atcggagtat gatgataaat gaaagatacc
     3901 tatgtgaaga aaggattggt tgtttctgtt atcctgttat tcatcggtgt cgcagtcgca
     3961 ccattcatca accagagtgt tgtcaaagca tctagtgatg atgacctggt ggaaatcacc
     4021 acacaggcat gtggtatcca gggatatggg aataatactg tgaaactcac cagggaacaa
     4081 taccaagacc tggaagcgta tctggttgag ttcagagcca ggttgaatca gacatcaaca
     4141 agggaggacg cagttcctat ctttaaggaa gcagtggtgg agttagataa gtatggattg
     4201 ttaccaaggg ggatgagtgt tgagacggca cagagattaa tattgaaaaa atataaaatg
     4261 atggatgaat tcggttcaag aaataagagt ttaaacatta atttaggttg tttggtcgcg
     4321 ggaaaggttg acttcgaaat aggtttttac ggacgattat tcctattact cagtagaata
     4381 tggtttttga cttttccgtt aatgttaatg ttatatatcc taagtttggt aaatcctgtt
     4441 tcgttggggg gagttatcac ttttggaagc catacgatat atcagcatgg ttctcaatat
     4501 ctaccagctg taggatgggt cactgcttta ggaattttag gtcaacagca ttggaatcac
     4561 tcattctatg gaaaaatttc accaattcaa atacttgtta aaggtggtga tcaaattgtc
     4621 tattattatg agttcattgg agcaataggt ttctgtggta ttaagattgt accatttggt
     4681 tttttcattg gttctgcctt ggcagtctct ttagattcgg agaaaccgat agaagtgaat
     4741 taactctaat aatctaaagt caatttaaaa gttgaatgat gcaaaaacca tctgaaattg
     4801 tcagatgatg ctgaggaata gctaccagac ttaatcctca ctaaccaaga gggtttttct
     4861 ttttaccaat gcttccaatg cttataattg gacaacaatg gttcgctggt gctcatggtt
     4921 gcaaatggct ggttttggtt tacacaaagt tatgctacag aaaaaccaaa ggactgaaaa
     4981 tggcacatct cagactatgc tgtaacttca attaatccag ggcaaccaag ttaaacattt
     5041 cgacaaaact atttatcgac cacacttcat aggggaaaac catgcaaccg acagagaacc
     5101 ttggcatcta caagaaaaac gatcgactct tcacggtcaa tccggaaaac tgcagaggaa
     5161 ccaaggtcta caacgaacaa ctcttcaccg aggatgacaa ggagtaccga tcctggaacc
     5221 cgtttcgcag caaactagct gcggcactcc tcaaaggaat cacccttgat ctttctccta
     5281 ctacaagtat cctgtatctg ggggcagcta ccggtacgac cgtcagtcat ctctctgata
     5341 tcgtcactaa aggcactctc tacgcggtag aatcctcacc gatcgcaatc atcagcttgt
     5401 tgaaggtcgc tgaaaaacga caaaacatca tcccgattct gtccgatgca aatcacccag
     5461 aacgatacca acatctggtc cctactgtgg acctggtcta tcaggatatc tcccagcgga
     5521 accaagcgga tatcttcaca cgtaacatac gagcctatct caaaccgaac ggaactgggc
     5581 ttatcatggt caaggcacgc agcatcgatg tcgcgctcaa accaaaacaa gcatatgagc
     5641 tcgtctgcag tgccctacaa aaagagaaat tcagaatgaa aaaaacagtg gatctaacac
     5701 cttttgaaaa ggaccatgca gcgattatca tgaacttata aaaaaaacta gatgttgatg
     5761 tagatttcct tcagcatctt gttgatgcta taataaggtg catcaagcag gaatgtttta
     5821 aactgattat ctgggagcaa tggagagttg cagatcttct gagctttatt gacatgtttt
     5881 gagatctctt ctaggtagag tttcttaatc tctggtgatt tcttttcgat cttttttcgt
     5941 atcgcccggt gttttaaatt gccgttcttt gcagtccgct tctcaagccg tgtcaacaga
     6001 ggaatgacga cgctgtcgat catctcacct ttctcaagat ccacgaggtc atcggcgagc
     6061 tgataagcaa aaccaacctc ccgcccatat tctgccagga cgtttgctat ctcctctgag
     6121 gattttgccc aataggctgc tgctttacat gctgcagaga acaaggttgc ggttttcaaa
     6181 tcgattatct tgctgtatac ttggaagaat ttagaatctg cagtgatatt gacatcctcg
     6241 aggtccttac tgttgaaatt aatctcggaa agctgaccgt caagggttcc gccccatgcg
     6301 tccacgtaca gtctcgctat tcggtccccg tgggacaatg caagattaaa ccccatgacc
     6361 agcatcttat gaccgatgag gatcgcgtta tcgattccgg tcttgatgta aaacgcaggc
     6421 tctccccgcc gggttaggtc accatcgatg atgtcatcat gaactaagga ggctccatgt
     6481 gcaagttcta ttgcgaccgt tccttctaaa aactgttggt agagttgtga tgattcattt
     6541 cccccggtac agactttgaa agacagggcg ccaagtaatg ggcgtaaccg tttcccctcc
     6601 tttaatatcg ttaaaatatt ctgatcagta ataagttcat tgattttctt ctcgatatcc
     6661 tcatgggtgt gtttgagaaa cacttcgaga gcaagtgatt ggtccggcat tgtttctacc
     6721 atttgataaa acttaactga tatcaactcc tatataatat acccttttat tagcttacta
     6781 tgatgaatca tggtactttt tacaagggtt tttgaacgat ttggcgataa tatagacctc
     6841 agagctactt ttacgtgatg cttcaggaga aaatgttttc acaaccataa attgaagttt
     6901 caccttttcg atgaatgctt tcgtatcttc tccttcaaag attttacaga taaaatggcc
     6961 gcctggttgt aatatctgtg tgaccgcgag taacgcgcat tcgcataacc atgcgcttcg
     7021 tgcttgatcg atagaataat tcccagagat gtcgggggac atatctgata atatcacgtc
     7081 tgcgttcttt ccattcatca ggctcttgat ttgctcaagg gtttcagttt tggtcacatc
     7141 tccttggagg aactgtatgc cctcgaaagg tttaatcgat gagaggtcta ctccgatgac
     7201 aataccatct gttcccacga gttcctttgc tacttggctc catccaccag gggctgcacc
     7261 aaggtcgact atgacaccat tcttcgggat cagatggaat ctgttttgga tttgttgtaa
     7321 tttgaaggcg gagcgtgcac ggtaaccgac tcgttttgcc tctttataaa aatgttcttt
     7381 ctttttttcc ttgtaccagc gagtcatagc gttttacaga atgatgtcga tgtcgagtcg
     7441 ttcagcaagt tccttgtatc ggttcctgat tgtgacttct gtcacaccag cgacatctgc
     7501 gacttctcgc tgtgttcgtc gttcgccaca gagaatcgat gagatataga tggatgcggc
     7561 agcaaccccg gttggtccac gaccactagt gagttccttg tcagcggcgt ctttcaaaat
     7621 ctcaattgct tttgcttgta catcgccgct gagtttcagt tcgctgcaga atcgtgagat
     7681 gtaatcctgg ggtgatgttg gcatgagttt taatcctaat tctcgggcga taaatcgata
     7741 ggttcttccg atttctttgc gggagactcg agaggatgct gcgatttcgt caagggttcg
     7801 tggtacgtta cattgtctgc aggcggcgta taaggctgat gctgatactc cttcgatgct
     7861 gcgtccgcgg atgaggtttt tgaggactgc ttttcggtaa accatcgcgg cggtttcccg
     7921 gacggtcctt gggagtccca taccggatgc catgcggtcg agttctgata aggcgaatgc
     7981 taagtttctt tcggttgcat cgctgattct gatgcgccgc tgccattttc gtaatcgata
     8041 gagttgagct ctgtttctgg tggggatgga ttttccgtag gagtctcggt ttttccagcc
     8101 gatcatggtg ctgagtcctt tgtcgtgtat ggtatacgtc attggtgctc cgacacgtgc
     8161 tcgtttttct cgttgatcgc tgtcgaatgc tctccattct ggtccatggt cgatgaagtc
     8221 ttggtcaata acaaggccgc aatcagcaca gacgagttct gcgcgtgcgt aatctttact
     8281 taaatgtgta ctgttacatt cagggcattg aatgatttct tcaatgcttt gtcttttttc
     8341 ttttttcttt ttggattgca tttgtaccat ttacctacgc ccccgtaaat tattttaaac
     8401 ttttcgatat gcttttaagc gcaatgatat aacttatata aatattttgc tatgtattat
     8461 tttttgaaat ataccttcag attcccaaga aaaatcagat aagactcatg aaaactactg
     8521 acataatgtt cttataatta tccccgaccg aaattattta agcattgggt ctgaatagac
     8581 tttgcatcca tattgcttgt tttcatcgaa tttgggtgca gactaaggct tttgggtgca
     8641 cagtagttcc gaaggtaaaa aaatgggaaa gaaacacgat tcatatggag aaaaattgcg
     8701 taaaccacta tcaaaaaatt caatctgtct tatcagaaaa atacgaaaga aatagctata
     8761 ataatt
//
LOCUS       DATMXJ010000207         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_24437, whole genome shotgun sequence.
ACCESSION   DATMXJ010000207 DATMXJ010000000
VERSION     DATMXJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24437"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /gene="grpE"
                     /locus_tag="VN377_05590"
     CDS             <1..287
                     /gene="grpE"
                     /locus_tag="VN377_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWR27890.1"
                     /translation="LDLHELLKKAYEDPDPKPGLKLLLENLDKFLEKEGVTYIDCKGK
                     PFNYQLHNAVSAIEQSECVDGTILDEIKKGYLVGEKLLRPAHVIVAKKKP"
     gene            314..2191
                     /gene="dnaK"
                     /locus_tag="VN377_05595"
     CDS             314..2191
                     /gene="dnaK"
                     /locus_tag="VN377_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWR27891.1"
                     /translation="MSKIIGIDLGTTNSEAAYIEAGKPVIIKSVEGQPYFPSVVAFTK
                     TGEMLVGEAAKRQAVTNTEGTVQRIKRKMGTHEKVTLRGKSYTPQQISAFILQKIKKD
                     AEDFLGEKITDAVITVPAYFNDDQRQATKDAGAIAGFNVKRIINEPTAAALAYGLDKE
                     GDQKIAVYDFGGGTLDVTIMEVGGGVFEVLSTSGDTQLGGSDMDRALVDYIAEEFKKK
                     HGVDLRKEPKTLQRVLEAVETAKIELSSTLQTDINLPYIYVVTGEPKHLEMKLTRTKL
                     EEIISPIVQRSEKPCRQALQDAKLKPEDIDHVVLVGGVTRMQIVQKKVEEIFKQKPKR
                     EVDPMECVAIGAAIQAGVLSGDIDKDIVLLDVTPLTLSIETLGGVATPLIDRNTTVPT
                     KKSKVFSTAADNQTSVEINVLQGERPMAADNKSLGRFHLDGILPAPRGLPQIEVSFDI
                     DVNGIMNVSAKDLGTGKEQSIRITGSTKLSDSEIERMRKEAKEHEDEDKRRKEKIEIR
                     NTADSLMHSTEKALVELKDKFSSEDKENLEKALKELREALTGDDTDVIKEKTDALTQA
                     FQKASTAIYQQAAQQYQQQPGQGSSQGESWQGQPSGDDTINADYKVKGEKKKKKDEDS
                     E"
     gene            complement(2203..2664)
                     /locus_tag="VN377_05600"
     CDS             complement(2203..2664)
                     /locus_tag="VN377_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWR27892.1"
                     /translation="MKTINRVVIPVDTTDLSKVAAEQGAFFAKLLNVEISIISVNDSQ
                     QYMLSKLLEEKIEKEKLTAIEEVKKIIEEQGIVTTTKLAAGAPAAEITKFVKDDDLIV
                     MASKGRKGFNKFFLGSVSEEVLKTAPCTVMVIKEKIKKWTVDDLTSLAKEK"
BASE COUNT          820 a          610 c          677 g          675 t
ORIGIN      
        1 tccttgacct acacgaactt ttgaaaaaag catatgaaga cccggaccca aaaccagggt
       61 taaaactttt actagaaaac cttgataaat tcctcgaaaa agaaggagta acctacattg
      121 attgtaaagg aaaacctttc aactatcaac ttcacaacgc agtctccgct atagaacaat
      181 ctgaatgcgt tgatggaaca atccttgatg aaataaaaaa agggtatctg gtgggagaaa
      241 aactcctccg gcccgcccat gttattgtcg caaaaaagaa accataaatt aaaccacttt
      301 ggaggtgtat gatatgagta agattatagg aattgatctc ggaacaacaa attccgaggc
      361 agcatatatc gaagcaggaa aaccagtcat tataaagagc gttgaaggac aaccatattt
      421 tccctctgtt gtcgcgttca caaaaaccgg ggaaatgctg gttggtgaag cagcgaaacg
      481 acaggcggtt accaacaccg aagggactgt tcaacgaata aaacgaaaga tggggacgca
      541 tgagaaagtc actctccgtg ggaagtccta taccccacag caaatctccg cgttcatcct
      601 tcagaagatc aagaaagacg ctgaggattt ccttggtgaa aaaatcaccg atgcagtaat
      661 aacggttcct gcctatttta atgatgatca gcggcaggca accaaggatg ctggtgcgat
      721 cgcagggttc aatgtgaaac gtatcatcaa tgagccgacc gccgccgctc tcgcctacgg
      781 gttagataag gaaggtgacc agaaaatcgc ggtgtatgat ttcggcggag ggacccttga
      841 tgtcaccatc atggaggttg gaggtggcgt tttcgaagtc ttatccacaa gtggtgatac
      901 acagctcggc ggctctgaca tggatagagc tctggtcgat tacatcgctg aagaattcaa
      961 gaaaaaacat ggcgtcgatc tccgtaaaga accaaaaaca ttacaacgag tattggaggc
     1021 agtagaaaca gcaaaaatag agttatcttc gacgcttcaa accgatatca acctacccta
     1081 catctacgtc gtcaccggag aaccaaaaca tcttgagatg aaactgactc gaaccaaact
     1141 tgaagaaatt atctctccga ttgtccagcg tagcgaaaaa ccctgtcgcc aagcgttgca
     1201 ggatgcgaaa ctgaaaccag aggatattga tcatgtcgtg cttgtcggtg gtgtcacccg
     1261 catgcagata gtgcagaaga aagttgagga gatctttaaa cagaagccga aacgggaggt
     1321 cgatccgatg gaatgtgtcg cgatcggcgc cgcgattcaa gcgggcgtgc tctccggtga
     1381 tattgataag gatatcgtac tgcttgatgt cactcccttg acgttaagca ttgagaccct
     1441 tggtggtgtc gcgacgccgt taattgaccg gaacaccacg gtgccgacga agaaaagcaa
     1501 ggtgttttcc accgctgcgg ataaccagac cagcgtggag atcaacgtcc tgcagggaga
     1561 acgacccatg gctgctgaca ataagagcct tggcaggttc catcttgatg gcatccttcc
     1621 tgctccgcgt ggactgcctc agatagaggt gtccttcgat attgatgtga acggcatcat
     1681 gaacgtgtct gcaaaggacc tgggtaccgg gaaagaacag tcgatccgca tcactggttc
     1741 gacgaaactg tcggattccg agatcgagcg gatgcgcaag gaagctaagg agcatgaaga
     1801 cgaggataaa cgtcgcaagg aaaaaatcga gattcgcaat accgctgatt cactcatgca
     1861 tagtacggag aaagcattgg tggaactgaa ggataaattc tccagcgagg ataaggaaaa
     1921 ccttgaaaaa gcgttaaagg agctgcgtga agcccttacc ggtgatgata ccgatgtcat
     1981 caaggagaaa accgacgcgc tgacacaagc gttccagaaa gcatccactg cgatttatca
     2041 gcaggcggca cagcaatacc agcaacaacc cggacaaggg tcgtctcagg gggaatcctg
     2101 gcaagggcaa ccttctggtg acgatacgat caatgcggat tacaaggtca aaggcgaaaa
     2161 aaagaaaaag aaggacgagg actctgaata acgtcctctt ttttattttt ctttggctaa
     2221 tgatgtgaga tcatcgacgg tccatttttt tattttttct ttgatgacca tcacagtaca
     2281 gggagctgtt ttgagcactt cttcggagac gctgcctagg aagaacttgt tgaagccttt
     2341 gcgtcctttg cttgccatca cgatcagatc atcgtctttg acgaatttgg tgatctctgc
     2401 tgctggtgcg ccagcagcta gcttggttgt tgtcactatt ccttgttcct caatgatctt
     2461 ttttacttct tctatcgcgg tgagtttttc cttttctatc ttttcttcaa gcagttttga
     2521 tagcatgtac tgctgtgagt cgttgacaga gataatggat atttcgacgt tgagtaattt
     2581 tgcaaaaaac gcgccttgtt ctgccgcgac ctttgacagg tcagtcgtgt cgacaggtat
     2641 gacaactcga ttgattgttt tcatccttca ccctcaacgg tattatgaat caaatgagac
     2701 tctttaaatg ttttgagcaa actcaatcgt tcgtgagaag aatggaaaca catcattttg
     2761 tttcggaaac aattgttcag at
//
LOCUS       DATMXJ010000208         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_61979, whole genome shotgun sequence.
ACCESSION   DATMXJ010000208 DATMXJ010000000
VERSION     DATMXJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61979"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="hxlA"
                     /locus_tag="VN377_05605"
     CDS             complement(<1..646)
                     /gene="hxlA"
                     /locus_tag="VN377_05605"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HWR27893.1"
                     /translation="MKPVLQVALDLLNADRALAIAHDAVKGGADWLEAGTPLIKSEGM
                     DIVRQLREQFPDKTIVADMKTMDTGALETEMAAKAGADIISILAASDDGTIRDAITSA
                     RKYGAKLMIDLISSPDMIRRAKEVEKLGAQFLCIHIGIDEQMHGKKPLSILKELVKHT
                     TLPVAVAGGLNSETVPEVVKAGAQIIIVGGAITKAKNVTEATRTIKKTMLEKKAC"
     gene            968..2050
                     /locus_tag="VN377_05610"
     CDS             968..2050
                     /locus_tag="VN377_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWR27894.1"
                     /translation="MKKIPFSQIRYIVLAGFCFVFILFAPFSGVFTAQSSRQSSSEPY
                     HYHTYQELTDELHSLQENYSALMSLSSIGKTYEGRDLWMVKLSDNVNQEEEEPGVLFM
                     AAHHGNEWPGVEICLFFIRYMLENYQNNSLPEVQNAMDTTQIYLIPMVNPDGVTADSR
                     KNCAPNHGPFGKSPTVTSYGVNLNRNYDDPWFLAYIFPMRYYLPIILPDSSFNYRGPY
                     PFSENETKAVKSFAENHDFSISISYHSYGEFIAYPWMHTTKQTPDEALFRSVGENISS
                     INDYYLLVKKDRLIPLYGGTLGSSENWLYREKGVLAYTVEVGEERRPTDPQVIHDLCM
                     TQTTVHLYLCQRAQTIESEKVVLHHS"
BASE COUNT          568 a          514 c          489 g          686 t
ORIGIN      
        1 gacaggcctt cttctcaagc attgtttttt tgatggtgcg tgttgcttcc gtgacgtttt
       61 ttgctttggt tattgccccg ccaacaataa taatctgagc accggctttg acgacctcag
      121 ggactgtctc actgtttaaa ccacctgcta ccgcaaccgg taatgtggtg tgtttgacga
      181 gttctttgag gattgacaaa ggtttttttc catgcatctg ttcgtcgatg ccgatgtgga
      241 tgcagaggaa ctgggcaccg agtttttcca cctctttcgc ccgtcgtatc atatctggtg
      301 agctgataag atctatcatg agcttcgcac catatttgcg tgctgaagta atagcatccc
      361 tgatggtacc atcatctgat gctgctagga tgcttatgat gtcagctccg gctttagccg
      421 ccatctctgt ttcaagagcg ccggtgtcca tggtcttcat gtctgcgacg attgtcttgt
      481 ccgggaattg ttctcgtaac tggcggacga tatccatccc ctcgcttttt atcagagggg
      541 ttcccgcctc aagccagtct gctcctcctt tcaccgcatc atgggcgata gcaagtgctc
      601 tgtctgcgtt cagaaggtct aatgctacct gcaagactgg tttcatcgaa tggagaatag
      661 gtgcgtctta tttaaaaagt aatgcatcgg gattcaaggg aatggagcgt cttttctagt
      721 gagatgagtc atctccctgg tcgtttggtt tttcccaaac gttttctgac aatatataaa
      781 gaatgaagga cataatctgg aattgaggtc ataaatatgg attcaggagt aaaacaattc
      841 cttgcattgc tgatcgtcgt atcaaccgtc gttgtccttg caaagatttt ccttcaattc
      901 taatccttta ttcttttaaa aaaattatat atacttcata tctatagtat ctatggggat
      961 acgttcaatg aagaagatac ccttctctca aattcgatat atcgtactag ctgggttctg
     1021 tttcgtattc atcctctttg ctcctttttc cggtgtgttc accgctcaat catctcgaca
     1081 gagttcatcg gaaccgtacc attaccatac ctatcaagaa ctcaccgatg agttacattc
     1141 cttgcaggag aactattccg ctctcatgtc gctttcctcg attgggaaaa cttacgaagg
     1201 acgggacctc tggatggtga agctgtctga taacgtgaac caggaagaag aagagcctgg
     1261 cgtcctgttc atggctgctc atcatgggaa tgaatggcct ggtgttgaga tctgcttatt
     1321 cttcatccgc tacatgcttg agaactacca gaacaatagc ctccctgagg ttcaaaatgc
     1381 catggacacc acacaaatct atctcatccc gatggtgaac cctgatggcg tcacggcgga
     1441 ctcgcggaag aactgtgccc cgaaccatgg accatttggg aagagtccga cagtgacttc
     1501 ttatggggtg aatctgaata ggaactatga tgatccttgg ttccttgcgt atattttccc
     1561 tatgcggtac tatctcccga ttattctccc tgattccagc ttcaattatc gaggtccgta
     1621 tcccttctca gaaaatgaga ccaaggcagt gaaatccttt gctgagaatc atgatttctc
     1681 catttcgatt tcgtatcatt cgtacggtga gtttatcgcc tatccctgga tgcataccac
     1741 caaacagaca ccggatgaag cacttttccg atccgtcggt gaaaacatat catccatcaa
     1801 cgactattat ctgttagtaa aaaaagatcg tctcatcccc ttgtatggag gcaccttggg
     1861 cagctctgag aattggttgt atcgcgaaaa aggtgtcctt gcgtataccg tcgaggtcgg
     1921 tgaggaacga cgcccaacag atccccaagt aatccatgat ctctgcatga cacagaccac
     1981 tgtacatctg tacctgtgtc agcgagcaca gaccatagaa tcagaaaaag tagtactcca
     2041 ccattcataa tttttttgat tttttcgatg tcttgttttt cataggaatt ttttgaattt
     2101 ataaaaaact ataaaaaagt acacaatata tatcccgctt gagacgagtt tttaatagca
     2161 atagtgagag aatggggtcg ttagtgaggt ggtgaggaga tgtgttttaa aaagactata
     2221 gcccccattc tctaataata tatattgatg acgcaag
//
LOCUS       DATMXJ010000209         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_77556, whole genome shotgun sequence.
ACCESSION   DATMXJ010000209 DATMXJ010000000
VERSION     DATMXJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77556"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VN377_05615"
     CDS             <1..1777
                     /locus_tag="VN377_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27895.1"
                     /translation="LLGSMVTSVTGTHSGTVKQLSFQPPIEWANTYGSEKIDWGNCVQ
                     QTSDGGYIITGALGRNAFMPWRGDIYMLKIDGNGEEQWSQTYGIAYNENVGRSIQQTS
                     DGGYIITGYTGYTYHIDGYVVKTDGNGNISWTSEFGNFDYYDNLQDASQTSDGGYIAV
                     GWTSSYGAGSADIWLIKMNSGGVEEWNHTFGGTGLDGGDHVQQTTDGGFIITGVIELT
                     GGNSNLILLKTDANGNEDWTKTFGGSEYEEGSFVQQTKDDGYIITGVTTSVGAGEGDI
                     WLIKTDSNGNVEWDYTFGGTANDQAHSVQQTTDGGYFIAGEYTNPTTQVPDMYVIKTD
                     ENGLEEWSDIIDNEGKEDVANYGIETTDCGYLVVGNTGVYQDELVDVWVMKYQGTNLP
                     PFEPANPSPADTSSDVPIDSDLSWTGGDPDNDSVTYDLYFGTNPSPLLFAENLPETSI
                     DPGTLSYLTTYYWMIRATDSFGAFTDGPVWSFTTEKDLPLLEIGTITGGKGVFAVIQN
                     NGNADASNVTWEIYITGGVFKLIYKSYGGLLTNIDAGNEETVTSGRCLGFGKIEITVS
                     ASCEEVPIPIEKKVTGSIFIIWIKM"
     gene            complement(1857..2180)
                     /locus_tag="VN377_05620"
     CDS             complement(1857..2180)
                     /locus_tag="VN377_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27896.1"
                     /translation="MVEKKKTTIKPEPAKTEGTKDETAKTPQSSYTMNEVFKITDDLF
                     TLSKEREYPLGAFIHGLIFTLEFASQSYRIPPQQLAEIKRDCRRYVDEIVRSNALRTT
                     EQKKK"
     gene            complement(2212..>2615)
                     /locus_tag="VN377_05625"
     CDS             complement(2212..>2615)
                     /locus_tag="VN377_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27897.1"
                     /translation="PTKETSFANITAGPICNITSGQFCQITTQPICQITSQPICSLTK
                     GFFCWTIYGPICPTTGIKCHPPTSRPVLCNIFGAAGKILKTIILVLLLATVIFVPIVI
                     LSMVFITMSNPERCEQIHEQITLWFNCTAPE"
BASE COUNT          776 a          518 c          645 g          676 t
ORIGIN      
        1 gttattaggc agtatggtta cctcagtgac aggaacccat agtgggaccg taaaacagct
       61 atccttccaa ccaccgattg aatgggcaaa tacatatggg agtgaaaaaa tagattgggg
      121 taattgcgta cagcagacct ccgatggcgg gtacatcatc accggggcac tcggcagaaa
      181 cgcctttatg ccctggagag gagatatcta catgctgaaa atcgacggaa atggcgaaga
      241 acaatggtct caaacttacg ggattgcata taatgaaaac gtcgggaggt cgattcaaca
      301 aacctctgac ggcggttata tcatcacagg atacacaggt tatacgtacc atatcgatgg
      361 gtatgtcgta aaaacagatg ggaatggtaa tatctcctgg acgtctgagt ttggaaattt
      421 cgattattat gataacctac aagacgcatc tcagacctct gatggtggat atatcgcagt
      481 cggatggacc agttcctatg gtgctggcag cgctgacatc tggctaatca aaatgaactc
      541 agggggagtc gaagaatgga atcatacatt tggagggacg ggtcttgacg gcggcgatca
      601 cgttcaacaa accaccgatg ggggattcat tatcactggt gtgattgaat taactggagg
      661 taacagcaat cttatcttac ttaagaccga tgcgaatggg aatgaggatt ggactaagac
      721 ctttggtggt tcagagtacg aggagggaag ttttgtacaa caaaccaagg atgacggtta
      781 tatcattaca ggtgtgacaa cctccgttgg ggcaggtgaa ggcgacatct ggttaattaa
      841 aaccgatagc aacggcaatg tcgagtggga ttatacgttc ggaggaacag ctaatgatca
      901 agcacacagc gtgcaacaaa caacagacgg tgggtatttc atcgctggag aatacaccaa
      961 cccaaccacc caagtccccg acatgtatgt gataaaaacc gatgaaaacg gactggagga
     1021 atggtcagat atcattgata acgaaggaaa agaagatgtt gcaaattatg gaattgagac
     1081 gactgattgt ggctatcttg tcgtcggaaa cacaggggtg taccaagatg aactcgtcga
     1141 tgtttgggtc atgaaatacc aaggcaccaa tctcccacct ttcgaaccag ccaatccatc
     1201 acccgcagat acatcaagtg atgttccaat tgatagtgat ctcagctgga caggtggaga
     1261 cccagacaac gactcggtga cctatgacct gtattttgga acaaaccctt caccactgct
     1321 atttgctgaa aatcttccag aaacctccat tgaccctgga acgctttctt atctaacaac
     1381 atattattgg atgattcggg cgaccgacag ctttggggcc ttcaccgatg gaccagtctg
     1441 gagctttacc acagaaaaag accttccatt gttagagatt ggtactatca cgggtggaaa
     1501 aggagtattc gcggtgatcc aaaacaatgg aaatgcagat gcaagcaatg tcacttggga
     1561 gatctatatt acgggtggtg tttttaaact gatttataaa agttatgggg gacttctcac
     1621 caacatcgat gcaggtaatg aagaaacagt cacatcagga cgatgcctcg ggtttgggaa
     1681 aatcgaaatc acagtgtccg cgtcgtgcga agaggtaccc ataccaattg aaaagaaggt
     1741 gactggaagt atcttcatca tctggataaa aatgtaaaca aaccatcggt caaatcaaat
     1801 gagcactaca ttagaatatc attggaagaa aaaaagaatt tcacaatcat ctttttttac
     1861 tttttctttt gctctgttgt tctgagagca ttcgaacgaa caatctcgtc aacgtagcga
     1921 cggcaatctc gtttgatctc cgcgagttgc tgtggtggga tacgatagga ttgtgaagcg
     1981 aattccaaag taaaaatcaa cccatggata aaagcaccaa gaggatattc tctttctttt
     2041 gaaagggtga agagatcgtc agtgattttg aaaacctcgt tcatcgtata ggatgattgt
     2101 ggtgttttcg ctgtttcatc ctttgttcct tcggtctttg ctggttcagg ttttattgtt
     2161 gtcttctttt tttcaaccat ttctctcatc tcacttaaag catgcatgat tttattcagg
     2221 tgcggtgcag ttgaaccaca aggttatctg ctcatgtatt tgttcgcacc gttctgggtt
     2281 agacatagtg ataaacacca ttgacaggat gacaatggga acaaaaatca ccgtcgcaag
     2341 cagcaggaca aggataatgg tctttaatat ttttcctgcg gcaccgaaga tgttgcacag
     2401 cacgggtctg gaggtcggag gatgacattt tatcccagtg gtggggcaga ttggaccata
     2461 gatcgtccaa cagaaaaacc ctttagtgag cgaacaaatc ggttgagaag tgatttggca
     2521 gattggttgg gtggtgattt gacagaactg gccgctggtg atgttacaaa tcggcccagc
     2581 ggtgatattt gcgaaagaag tttccttagt tggac
//
LOCUS       DATMXJ010000210         6496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_4275, whole genome shotgun sequence.
ACCESSION   DATMXJ010000210 DATMXJ010000000
VERSION     DATMXJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4275"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VN377_05630"
     CDS             complement(<1..546)
                     /locus_tag="VN377_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR27898.1"
                     /translation="MPNIENLEPKLVWKHFDEIRKIPRCSKHEEKIREYIVNFAKKQG
                     LKTKVDQVGNVVILKPATTGMQNKPTVILQGHMDMVCEKNSDVIFDFSKDPIQLKITG
                     DLLTANGTTLGADNGIGLAISLAILEDTKVRHGPIEALYTIDEETGLTGAFAMKSDML
                     TGKIMLNLDSEDFGVLTVGCAG"
     gene            complement(679..1296)
                     /locus_tag="VN377_05635"
     CDS             complement(679..1296)
                     /locus_tag="VN377_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27899.1"
                     /translation="MSDRKEVPIGIVVFLIFIIVLDAIFLFYLGNALLFEYSDYIAFF
                     SFSLMSIVMWIDVLLTVLSLIIIPYGFVKKKNWARIFAFVFLSWLLLRTILYIATTGE
                     KTIGYLFFVLFIVSLMYLLSSSVKSYFKISTMAIIPTEIIQEYTYGPYTLYTELVHLR
                     NGKNQIIYFFSKRKPKSGTPTTLPEGYLVEVSKRSGLPYLKKETS"
     gene            1464..2246
                     /gene="lsrF"
                     /locus_tag="VN377_05640"
     CDS             1464..2246
                     /gene="lsrF"
                     /locus_tag="VN377_05640"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HWR27900.1"
                     /translation="MDWGMKNRLSRIIKPKDGRTVMLAVDHGYFMGSTSGLERLNCMV
                     NPLLPYTDTLMLTRGALRDYINPTIDVPIVLRVSGGTSIIGPKLLHEGTVVSIEDAIR
                     LNASGVAYSILVGAEFERDTILGMTRYIDEAERYGIPVLAVTAVGKEMKRDARYMSLA
                     SRIAAELGAHIVKTYYVPEFEKVIESCPVPVVIAGGKKQPEDEALQMAYDAIQAGAIG
                     VDMGRNIFQSLNPVNMIKAVNEIVHKKATPKEAYEVFKGKKK"
     gene            2326..2889
                     /locus_tag="VN377_05645"
     CDS             2326..2889
                     /locus_tag="VN377_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWR27901.1"
                     /translation="MDLTLIIGLIVLLIVVVVLLWFFGYYNRVVRYENRIDNAWAQID
                     VQLKRRADLIPNLMETVKGYMNHERQTLEAVTQARSAVMTAKSPQESINADNMLTGAL
                     KTLFAVSESYPDLKANQNFLQLQDELTHTEEKIAYSRQHFNDSVLQFNNLIETFPGNI
                     FANMMGKKERQMLQIPEASREVPKVSF"
     gene            2912..3823
                     /locus_tag="VN377_05650"
     CDS             2912..3823
                     /locus_tag="VN377_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWR27902.1"
                     /translation="MEQQPRLIEDHIRKNKFDTLVICMFMVILLFSVIFAIGFILGAP
                     PILTIMLALPIALFYIFITYSFSVQSVISAAGARPVNPQNREEKLLSFKVEEMALAAG
                     LPTPKVYVQDSKDINAFATGHKPEDAVICATTGALQQLNSEELEGVIGHEMSHIRNRD
                     ILVMTVTVGVVGAIALIAEIALRMMFFTRGGRSNDRKGDGNAIILVIAIIFIILAPIF
                     SRLTYLAISRRREYLADADGAYLTRNPEGLAKALEKIKGDLPDDPKGSKTVAPLYIAN
                     PYKRALRDSIWSTHPPLDERIRRLRSM"
     gene            3957..4763
                     /locus_tag="VN377_05655"
     CDS             3957..4763
                     /locus_tag="VN377_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27903.1"
                     /translation="MKKTLTIILLTILTITVLCPSIRANDAGVIVDQEIIAISLSNTG
                     LQVDETIKVTNIGTENVTSLRFWIQQDVQDAVEIKEKQSGTDIIPLITGNIRTCNLSA
                     VNLSMTPDTSLTLQVTYSLPNNAQNFVKTIQYNTTMFTISYENRDIFRGENLLYASDM
                     NNEIQIRLYNPTEAPLNITMIILVFLVVIIVLAVLLLVLKKQRSKTKKAVSESEETLV
                     TKKSLLLSLLKDLEKQYRAKSISDETYTKIKDEYKQDAVDVMKKLDDLKK"
     gene            complement(4767..6206)
                     /locus_tag="VN377_05660"
     CDS             complement(4767..6206)
                     /locus_tag="VN377_05660"
                     /inference="COORDINATES: protein motif:HMM:NF003229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HWR27904.1"
                     /translation="MEDWTEKYRPKTLRDIIGNERALSALRSWAERWNQGKLPDKRAA
                     ILSGKPGTGKTSTAHALASDLGWTVLELNASDARNESSIKRVATAGAVNETFDITGEF
                     IPLRKGGRKLIILDEADNLYEKLDKSDSESGLGDRGGKKAIIDTIRITRQPIILIVND
                     FYELTKGGGEPLKNLCNVIQFYTVNPSQIVELLKHICIQENISADIRALKTIVDRSKG
                     DVRSAVNDLQSLCVNRTQLTMEDIDVLGYRDRDALIFDALREVFKTKNFQKSRDCMRD
                     IDVEPEMFLLWITENLPREYLDVKDLVRGYEALSTADVFFGRVAKTRVYDLWSYACDM
                     MSAGVSTAKTHSYGNTQYAFPLWLKQMKGNQSARLIRDGVTKKISRLNHVSDQKTKEA
                     ILPHFQALFRNNPRFACKMIRQLDLVESEVKFLLGEKYLHRMKDIVQCAEKTDEKQEE
                     IEMTTPMKQKDGEEKKESTDLKQPSIFDF"
     gene            6295..6377
                     /locus_tag="VN377_05665"
     tRNA            6295..6377
                     /locus_tag="VN377_05665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6329..6331,aa:Ser,seq:gct)
BASE COUNT         1847 a         1513 c         1353 g         1783 t
ORIGIN      
        1 accagcacat ccgacagtaa gcaccccgaa atcctcgctg tcaaggttga gcatgatttt
       61 cccggtcagc atatccgatt tcatggcaaa cgcaccagtc agaccggttt cttcatcaat
      121 cgtgtataat gcttcgatag gcccgtgtcg caccttagta tcctctaaga tggcaaggct
      181 tatcgcaagg ccgatcccgt tgtctgctcc aagtgttgta ccgttcgcgg taagcaggtc
      241 accggtgatt ttcagctgaa tcggatcttt agaaaaatca aatattacgt cactgttctt
      301 ttcacagacc atatccatgt gtccctgcag gatgacggtc ggtttgtttt gcatccctgt
      361 tgtcgctggt tttagaatga cgacgttccc aacctggtct acctttgttt taagtccttg
      421 tttcttagca aaattcacaa tgtattccct gattttttcc tcgtgttttg agcagcgtgg
      481 gattttccta atctcatcaa aatgtttcca tacaagtttt ggttctaaat tctcaatatt
      541 tggcataagc tgcctccctt attgtacgga ctggtaacac cacaacacat aaaaaaggtt
      601 tgccaaaaga aaaaaaagga tgtccccgcc aaagagattc tgatgttgtt tataaaatta
      661 tgacacaatg tttagaactc aactcgtctc ttttttcaaa tacggcaaac ctgacctctt
      721 gctcacttcc accagatatc cctccgggag cgtggtggga gtcccgcttt taggttttct
      781 tttactaaaa aaatagatga tttggttttt cccatttctc agatgaacga gttctgtata
      841 taacgtatag ggcccatagg tgtattcctg tatgatttcc gttggaatga tagccatggt
      901 acttatcttg aagtaacttt tcactgatga cgaaagaaga tacatcaagg atacgataaa
      961 cagaacaaaa aaaagataac caatggtttt ttcgccagtc gtagcgatgt acaggattgt
     1021 tctcaaaagt aaccaggaga gaaacacgaa cgcaaagatt cgcgcccaat ttttcttttt
     1081 cacaaaacca taagggataa taatcaaaga aagtactgtg aggagtacat caatccacat
     1141 tacgattgac ataagggaga atgaaaaaaa cgcgatataa tcactatact cgaatagaag
     1201 agcattccct agatagaata ggaaaatagc atcaagaaca atgatgaaga tgagaaatac
     1261 tacgatcccg attggaacct ccttcctgtc gctcattgat agaaataaat atcgaaacga
     1321 gacttatatt tttccactct aatcatatta cagtaactac ctgaaaataa ccatgatgaa
     1381 agaaaatctt ctatccctaa acatcgacag taatagttta tataatcctg cgtcgtttac
     1441 caggaaaacg gaggcaaaag agtatggatt ggggtatgaa aaatcggctt tcccgaatca
     1501 ttaaaccaaa agatggacgc accgtgatgc ttgcagttga tcatgggtac ttcatgggct
     1561 cgacttcagg gcttgaacga cttaattgca tggtgaatcc tttgctgccc tatacagata
     1621 ccttaatgct gacacgtggt gcgctacgag actacatcaa cccaacgatt gatgttccca
     1681 tcgtcttacg cgtctcaggg ggaacaagta ttatcggtcc gaaacttctc cacgaaggca
     1741 ctgttgtttc cattgaggat gcgatacgac tcaacgcctc cggtgtggca tactctatcc
     1801 tagtcggagc agaattcgag cgggacacca tcctaggcat gacccggtac atcgacgaag
     1861 cagaacgata cggaatccca gtcttagccg taactgcagt gggaaaggaa atgaaacgag
     1921 acgctcgcta catgagtctc gcgtcccgaa tagctgctga acttggtgcg catatcgtaa
     1981 aaacctatta cgtccctgag ttcgagaaag tcatagaatc ctgtccagtc ccggtcgtca
     2041 tcgcaggtgg gaagaaacaa ccggaggacg aggcgctaca gatggcgtac gatgcgattc
     2101 aggcaggcgc aatcggtgtc gacatgggaa gaaacatctt ccaaagcctc aacccggtca
     2161 atatgataaa agctgtcaac gaaattgtcc ataagaaagc gacaccaaag gaagcctacg
     2221 aggttttcaa agggaaaaag aaataatgat tccccatgtc ctatcttttt acacgatatg
     2281 tttaaataaa acatcttatt acacctatcc ctgaggaggc aaactatgga cctaactttg
     2341 ataataggac taatcgtact actgatagtt gttgtagttc tgctctggtt ctttggttac
     2401 tacaaccgag tcgtcaggta cgaaaacaga attgataacg catgggctca gattgatgtg
     2461 cagctcaaac gaagggcaga ccttatcccc aatctcatgg agaccgtcaa aggttacatg
     2521 aatcatgagc gacaaaccct cgaagccgtg acacaagccc gatctgcggt tatgacagca
     2581 aaatcacctc aagaaagcat caacgcagac aacatgctga ccggagcgtt aaaaacatta
     2641 tttgcagtat cggaaagcta ccctgacctc aaggcgaatc agaatttcct gcagcttcag
     2701 gacgaattaa ctcacactga ggaaaaaatc gcatactcac ggcagcactt caatgactcc
     2761 gtactacagt tcaacaacct tatcgaaacc ttccccggca atatctttgc gaacatgatg
     2821 gggaagaaag aacgacaaat gctacagatc ccggaagcat cacgagaagt acctaaggta
     2881 tcattctaag taagtcgatt ctctgaaaat tatggaacag caaccgcgac tcatagagga
     2941 ccatatacgg aaaaacaagt tcgataccct tgttatctgt atgttcatgg tcattctcct
     3001 tttttctgtt atttttgcca tcggctttat cctgggtgct ccacccatac tgaccataat
     3061 gcttgcgctg ccaatcgcgc tgttctacat ctttattacc tattcatttt cagtacaaag
     3121 tgttatttct gcggcagggg cacgaccggt gaacccacag aaccgcgaag aaaaactcct
     3181 gagctttaaa gtcgaagaga tggctcttgc tgctggccta ccaacgccta aggtctatgt
     3241 tcaggattca aaggatatca acgcgttcgc gaccggccat aaacctgagg atgccgtcat
     3301 ctgcgcgacc accggtgcct tgcagcagtt gaactctgaa gaacttgaag gcgtcattgg
     3361 ccatgagatg agccatatcc ggaatcgcga tatcctggtg atgactgtca ccgtcggagt
     3421 cgtcggggcg attgccctta tcgctgagat cgcgttacgg atgatgttct ttacaagggg
     3481 tggacgaagc aatgatcgaa aaggagacgg caatgccatt attcttgtca tcgccatcat
     3541 cttcattatc ctcgcaccga ttttctcaag gcttacctac cttgcgattt cacgcagacg
     3601 agagtacctt gctgatgctg atggagcgta cctgacgaga aacccagagg gactcgcaaa
     3661 agctttggag aaaatcaaag gggatttacc tgacgatccg aaaggctcaa agacagttgc
     3721 gccactctat atcgcaaatc catacaaacg agcgctccgt gactcgattt ggtcgaccca
     3781 tccacctctt gatgagcgga tccgccgcct gcgatcgatg taacctcatt ccttctttta
     3841 ataaaacaaa accacgaagg aaatacaaca atactctata aattgtgcag caaccttgtg
     3901 gagaaaaaca atcgaaaact acataaacaa gaaacacatg gcaacggaga atccatatga
     3961 agaaaacact aacaatcatt ctgctcacga tactaacgat caccgtcctg tgtccttcaa
     4021 tacgtgcgaa tgatgcagga gtgattgttg accaagaaat catagcgatt tctctttcta
     4081 acaccggtct gcaggtcgat gaaaccatta aagtgaccaa tatagggaca gagaacgtca
     4141 cctcactgcg tttctggata caacaagatg tccaagatgc tgtggaaatt aaggagaagc
     4201 aatccggcac agacatcatt ccactgatta ctgggaatat ccgaacgtgt aacctcagcg
     4261 cggtaaatct ctcgatgacc cctgatacgt ctctgactct gcaggtgacc tattctttac
     4321 caaacaatgc ccagaatttc gtaaaaacta tccaatataa tacgacgatg tttaccatct
     4381 cctacgagaa tcgggatatc ttccggggtg aaaatctttt atatgcctct gatatgaaca
     4441 atgagataca gattcgttta tacaacccga cggaggctcc gctaaacatc acgatgatta
     4501 tcctcgtctt ccttgtcgtc attatcgttt tagctgtgtt gttactcgta ttgaaaaaac
     4561 aacggtcgaa aaccaaaaaa gcggtctccg agtcagaaga aacactcgta acgaagaaat
     4621 cattgctgct ctcactgtta aaggaccttg agaaacagta ccgtgcgaaa tccatttctg
     4681 atgagaccta caccaagatt aaagatgagt acaaacaaga cgctgtcgat gtcatgaaaa
     4741 aactggatga cctgaagaaa taacgattaa aaatcaaaga tgcttggttg tttcagatcc
     4801 gtgctttctt ttttctcctc tccatctttc tgtttcattg gggtcgtcat ttcgatttct
     4861 tcttgtttct catcggtttt ttcagcgcac tgcacgatgt ctttcatccg atgaagatat
     4921 ttttccccca gaaggaattt cacttcgctt tctacgaggt ccagctgccg tatcatcttg
     4981 caagcaaatc tggggttatt ccgaaacaag gcctggaaat gcggaaggat cgcttctttc
     5041 gttttctggt ctgagacatg attcagcctt gagattttct ttgtcacacc atcacgaatc
     5101 aaccgcgcgg attggttacc tttcatctgt ttcaaccata acgggaatgc gtattgtgta
     5161 ttaccatagc tgtgggtttt tgctgtagag acccccgcac tcatcatatc acaagcgtac
     5221 gaccagagat cgtagacccg tgttttggcg actcttccaa aaaacacatc tgctgttgag
     5281 agtgcctcgt agccacgtac gagatccttc acatcgagat attctctggg taaattttca
     5341 gtgatccaga gtaaaaacat ctccggctcc acatcgatat ctcgcataca atctctgctt
     5401 ttttggaaat tttttgtttt aaacacctct cgtaacgcat cgaaaatgag agcatctcgg
     5461 tcacgatatc cgagcacatc gatatcctcc atggtgagtt gtgtacggtt gacacagagt
     5521 gattggaggt cgtttaccgc agatcggaca tcccctttgg accggtcgac aatggttttt
     5581 aatgctcgga tgtccgccga aatattttcc tggatacaga tatgttttaa caactcgacg
     5641 atctgactgg gattcactgt gtagaattgg atgacgttac agaggttttt aagtggttct
     5701 cctccacctt tcgtcagctc ataaaaatca ttgacaatca agatgattgg ctgtctggtg
     5761 attctgatcg tatcaatgat cgctttttta cctcccctgt ccccaaggcc actctctgaa
     5821 tctgatttgt caagtttttc gtacaggtta tctgcttcat caagaatgat gagttttcgt
     5881 cctccctttc tcaatggtat aaattctcca gtgatatcga atgtctcatt gactgcaccc
     5941 gctgtcgcga cacgtttaat ggatgattca ttgcgtgcgt ctgatgcgtt gagttcaaga
     6001 accgtccatc caaggtctga agctaacgca tgagcggtac tggtttttcc tgtccccggt
     6061 tttcctgaga ggatcgcagc ccgtttatca ggtaattttc cttggttcca tcgttctgcc
     6121 caagaacgta aagcagaaag agcacgctca ttacctatga tatcacgtaa tgtcttcggt
     6181 cggtatttct ctgtccagtc ctccattggt gaccgagaag attcataggg tattttaaga
     6241 tttgttttga cataaccttt ttatcggtct ggtttattat ctcccactcc gaatgcgggg
     6301 atggcccagc ccggtaaggc gttggattgc taatccaatg gtctcagacc tcgggagttc
     6361 gaatctccct ccccgcgtaa atatttatcc tggttataat tagcacgatc gttaaaatgg
     6421 agatacatat taagatcaat caaagagtgg tattattccc ttttttcttt ctaagattcc
     6481 tcgtaccttc taatgg
//
LOCUS       DATMXJ010000211         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_20026, whole genome shotgun sequence.
ACCESSION   DATMXJ010000211 DATMXJ010000000
VERSION     DATMXJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20026"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3107
                     /gene="carB"
                     /locus_tag="VN377_05670"
     CDS             <1..3107
                     /gene="carB"
                     /locus_tag="VN377_05670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HWR27905.1"
                     /translation="SQAIKAIKDEGIKIIVVNPNIATIQTSEYLADKVYFLPVTAAFV
                     KKVIAKERPDGILLGFGGQTALNVGLMLAETGVLKKYNVEVLGTPILSIQNTEDREQF
                     VKKIKEINLKVPVSRAVNTVDAAIHVAEEIGYPVMCRIAYALGGLGSGMAANKKELVD
                     LAKKAFSYTRQILIEEYLGGWKELEYEVVRDRYDNCIVVCSMENVDPMGIHTGESIVT
                     APVQTLSASENFMLRSLSIKVIRHLGIVGECNIQFALDPQSEDYRIIEVNARLSRSSA
                     LASKATGYPLALIAAKLSLGYGLPELPNMITGETSACFEPALDYVVVKFPRWDLQKFR
                     RVSIRLGSEMKSVGEVMAIGRSFEEALQKAIRMLDVGTPGLVGDTLVFANLEKELREP
                     SDKRMFAIVQALKQGYSLEKVAKISKVTPWFLSKIQNIIDIEQQLKNHSLSSLPSALF
                     REAKQKGFSDQQIALFTKSSESDVRKRRKKLHIIPNIKQIDTLAAEYPAKTNYLYLTY
                     NATTDDLDKVEKHQVMVLGGGAYRIGSSVEFDWCCVNSISTLRTLKYHTIMINCNPET
                     VSTDYDICDKLYFEELTLERVLDIYEKEKPQGVIISMGGQIPNTLALPLFEAGVPILG
                     TSPQDIDVAEDRHKFSALLDALKVDQPQWEELTSVESAEDFAATVGYPVLVRPSYVLS
                     GAAMSIALSNKELRAYLEKASEISKKHPVVISKFITGAKEIEIDAVADHGKLYCYAIS
                     EHVENAGVHSGDATIVLPPQRTYLETMRRVKTITKSIATSLQITGPFNIQFIAKDNDV
                     KVIECNLRASRSFPFVSKVFKINFIDFATKIIMGKRVPRIDRSAFDLDYVGVKASQFS
                     FTRLKGSDPILGVEMVSTGEVACLGDDFNEAFLKSLLSVEFMIPKKTILLSTGPVDSK
                     ADFLSSANILQAMGYRFYATKGTADFMKENGMTVEVLYWPSEGKEPNTLTYIAEGKID
                     LVINIPKNIEKEELENDYLIRRKAVDFNVPLITNLQLAKRFVEALSRTDIDDLKIKSW
                     NEYV"
     gene            complement(3113..3289)
                     /locus_tag="VN377_05675"
     CDS             complement(3113..3289)
                     /locus_tag="VN377_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HWR27906.1"
                     /translation="MKREVIKILCCPTCKGDLALTVEKEEKGDIISGTFTCAHCHITY
                     PITDGIPDLLPRET"
     gene            complement(3304..4383)
                     /locus_tag="VN377_05680"
     CDS             complement(3304..4383)
                     /locus_tag="VN377_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWR27907.1"
                     /translation="MVDIYQYDKKKEEQTIIKTDTGRRKKEQYIENLREGDAINDFFA
                     VKLKKAQRPYKRGMFFEFVATDKTGEISVKYWGGENKDRVKRLYESFNTGDVVQVRTG
                     MVENYEDRLQVSVNENTGGVRKCAPNEYDVIDFLPALPEERIEELHDIIKKELKTIQS
                     EPLKHLLASFFNDPEIVKMYLHSPSAISRHHNYIGGNLEHTVGVIRLCQNISEMYPSV
                     NKDLLLSGALLHDIGKLKEYTYAAAIDVSDEGNFIGHIVIGEQWIREKIQVLRTNGEE
                     FPKELEHQLIHLILSHHGKYEWGSPKLPKLLEACILHQADLMDSQVKNYLQMIQDAKK
                     LTDEDWTFVYDADAGKKRAIFLGEY"
     gene            complement(4420..>4986)
                     /locus_tag="VN377_05685"
     CDS             complement(4420..>4986)
                     /locus_tag="VN377_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HWR27908.1"
                     /translation="AKDLGKILVINFKHACFLEQYRQDKKLQVPPITDPHLALLKPKR
                     LTGTYINEDYTDTYIKKRLHYKNIITAADITKNPSQYIIVLNFWYFSDLIDLKPKNGL
                     YIHSLSEPFNEEMEISYDRMKHWLTHFDIELFQSHCSGHINGDDLKELIKTVNPMKLY
                     PIHTEYPKLFKKLPSKTIMVKEGKTYKL"
BASE COUNT         1380 a         1062 c         1131 g         1413 t
ORIGIN      
        1 gtagtcaagc gattaaagcg ataaaggacg aaggaataaa aatcattgta gtcaatccga
       61 atattgcgac aatccaaacc tctgagtacc ttgctgataa agtctatttt ttgccagtga
      121 ctgcagcgtt tgtcaaaaaa gtaatcgcga aggaacgacc agatggtatc ctccttggtt
      181 tcggaggaca aacggcgctg aatgttggac ttatgcttgc ggaaaccggt gtgctgaaaa
      241 aatataacgt cgaggttctc ggaaccccga ttctctcaat tcagaacaca gaggatcgag
      301 aacaatttgt gaagaaaata aaagaaatca acctaaaagt ccccgtcagc agagcggtca
      361 ataccgtcga tgcagcgatt catgtcgctg aagaaatcgg atatcccgtg atgtgccgga
      421 tagcgtacgc gcttggtggt ctgggctctg gtatggcagc aaataaaaaa gagctggtgg
      481 acctggcgaa aaaagctttc tcgtacactc gtcaaatcct tatagaggaa tatcttggag
      541 gatggaagga gctggaatat gaggtggtac gggatcgata tgataactgc atcgtggtct
      601 gttctatgga gaatgtcgat ccaatgggga ttcacaccgg ggaaagcatt gtcaccgctc
      661 cagtacaaac cctttctgca tctgagaatt tcatgttacg ctccctttct ataaaagtca
      721 tcagacatct tgggattgtt ggtgaatgta acatccaatt cgctttagac cctcaatcgg
      781 aagattaccg tatcatcgag gtgaacgcgc gactcagtcg tagttctgcg cttgcatcaa
      841 aggcaaccgg atatccactc gcattaatcg ctgcaaaact cagtcttggc tatggactcc
      901 ctgagttacc gaatatgata accggagaaa cctctgcgtg tttcgagcct gctcttgatt
      961 atgtggtcgt gaaatttccc cggtgggacc tacagaagtt cagacgagta agcatccgac
     1021 tgggatctga aatgaaatca gtcggcgaag tcatggctat cgggaggagc tttgaggaag
     1081 cattgcagaa agcaatccgg atgcttgatg tcggaacacc tggccttgtc ggagacactc
     1141 tggtatttgc taatctcgaa aaagaattaa gagaaccttc cgataagcgc atgttcgcga
     1201 tcgtccaggc actcaaacaa ggatactccc ttgagaaagt cgccaagata tcaaaagtga
     1261 ctccatggtt cctttcgaag atacaaaaca ttattgatat tgaacaacaa ctaaaaaatc
     1321 acagtctatc ttctttacca tccgcattat tcagagaggc caaacaaaaa ggtttttcag
     1381 atcaacaaat agccctgttt acaaaaagca gtgaatctga tgtacggaaa cgacggaaga
     1441 aacttcacat cattccaaac ataaaacaga ttgacaccct tgctgctgag taccccgcaa
     1501 aaaccaacta cctctacctc acttacaatg caactactga tgaccttgac aaggtcgaga
     1561 aacatcaggt gatggttctc gggggtggag cctaccggat cggctcatcg gtggaatttg
     1621 actggtgctg cgtgaattct atctcaacac tacgtacatt gaagtatcat accatcatga
     1681 ttaattgtaa ccctgagacg gtcagcacgg attatgatat ctgcgataaa ctctattttg
     1741 aagaattgac cctggagcga gtcttagata tctatgagaa ggaaaaacca caaggagtaa
     1801 ttatttcgat gggtggccag atccccaata ccctcgcgct cccactattt gaagcaggtg
     1861 tccctatcct aggaacctcc ccacaggata tcgatgtcgc agaggatcgt cataaatttt
     1921 ccgcattatt ggacgcttta aaggtggatc aaccacaatg ggaagaactc acaagtgtag
     1981 aatcagctga agattttgca gctaccgtag ggtatccggt gttggtacga ccaagttatg
     2041 tcctcagcgg tgctgcaatg agtatcgcac tcagcaataa agaactccgt gcatacctcg
     2101 agaaagcatc agagattagt aaaaaacacc ctgtcgtcat cagtaaattc atcaccgggg
     2161 caaaagaaat cgaaatcgac gcggtagcag accatgggaa actctactgt tatgccatct
     2221 ccgagcatgt tgaaaacgcc ggggttcatt ctggtgatgc gaccatcgta ctgcctccac
     2281 agcgaaccta tttagagacg atgcgtcggg tcaaaaccat tacaaagagc atcgcaacct
     2341 cattacagat tacaggaccg ttcaacatcc agtttatcgc aaaggacaac gatgtgaaag
     2401 tcatcgaatg caatctacgt gcatcgcgaa gcttcccatt cgtctcaaag gtctttaaga
     2461 ttaattttat tgattttgcg acgaaaataa ttatgggaaa acgagtccct cgcattgatc
     2521 gatctgcttt tgatttggac tatgtcggtg tcaaggcatc tcagttttcg ttcacacgat
     2581 tgaaaggatc agacccgatt cttggcgtcg aaatggtatc aacaggtgaa gttgcttgcc
     2641 ttggcgatga tttcaatgag gcattcttga aaagtctact gtctgtcgag ttcatgatac
     2701 caaaaaaaac tattcttttg tccacaggac ctgtcgatag caaagcagat ttcttatcga
     2761 gtgccaacat attgcaggcg atgggttatc gtttctatgc gacgaaagga actgctgatt
     2821 tcatgaagga aaatggcatg actgtggaag tcttatactg gccatcagag ggaaaagaac
     2881 cgaacacgct cacctatatc gctgaaggga aaatcgattt agtgataaac atccccaaaa
     2941 acatcgaaaa ggaagaactt gagaacgatt atctgattcg tcgaaaagct gttgatttta
     3001 atgttccttt gatcacaaac ctgcaactcg cgaaacgctt cgttgaagct ctttctcgaa
     3061 cagacatcga tgaccttaaa ataaaaagct ggaatgaata tgtctaagag acttaggttt
     3121 ctctggggag caggtcaggg atcccatcgg tgattggata cgtaatgtga caatgggcgc
     3181 aggtaaatgt acccgaaata atgtctcctt tttcctcttt ttcgacagta agagcaaggt
     3241 ctcctttgca ggtcggacag cagaggattt tgatgacttc tcgtttcata gtaccatcct
     3301 taatcagtat tcacctaaaa atattgctcg cttttttcca gcatctgcat catacacaaa
     3361 cgtccagtct tcatcagtga gtttctttgc atcttggatc atctgcaagt aattttttac
     3421 ctgagagtcc atcaaatcgg cctgatggag aatacaagcc tccaggagtt tcggtagttt
     3481 tggcgagccc cactcgtatt tgccatgatg actcaggata agatggatca gctggtgttc
     3541 taattccttt gggaattcct ccccgttcgt tctgagtacc tggattttct ccctgatcca
     3601 ttgttccccg atcacgatat gaccgatgaa gttaccttca tcgctgacat cgatagcagc
     3661 agcgtaggtg tactctttga gtttaccaat gtcatgcagt aatgcaccag aaagcagaag
     3721 atctttattg acagatgggt acatttcaga gatattctga cagaggcgga tcacaccgac
     3781 agtatgttca aggttaccgc cgatataatt atggtgtcgc gagatagccg agggggaatg
     3841 gagatacatc ttgacgatct ctgggtcgtt gaagaaggat gcaagcaggt gtttcaatgg
     3901 ttcactctga atggttttta gttctttttt gatgatatca tgaagctctt caatgcgttc
     3961 ttcaggtaat gctgggagaa aatctatgac atcgtattca ttaggagcac attttcgtac
     4021 tccaccagtg ttttcattca ctgagacctg cagtctatcc tcatagtttt caaccatgcc
     4081 cgtccgcact tggacaacat cccccgtgtt gaaactttca tagagccgtt tcactcgatc
     4141 tttattctca ccgccccagt atttcacgct gatctcacca gttttatccg tcgcaacaaa
     4201 ctcaaaaaac attccacgtt tgtagggtcg ttgggctttt tttagcttca cggcgaaaaa
     4261 atcgttgatc gcgtcgcctt ctctcaggtt ctcaatgtat tgttctttct ttcgccttcc
     4321 tgtatcggtt ttgatgatgg tctgttcctc ttttttcttg tcgtattggt agatatccac
     4381 cattatggta acctcccatg acttcttcct tgatactgtt tagagtttat aggtttttcc
     4441 ctctttgacc atgatggttt tactagggag ttttttgaat aattttggat attccgtgtg
     4501 gatcgggtag agtttcattg gattcactgt tttgatgagc tctttgagat catcaccgtt
     4561 gatatgtcca gagcaatggg attggaataa ttcaatatca aaatgggtca gccagtgctt
     4621 catccggtcg tatgagattt ccatttcttc gttgaatggc tcggagagcg aatgaatgta
     4681 caatccattt tttggcttga ggtcgataag gtctgagaag taccagaaat tcaacacaat
     4741 tatgtattga gagggattct tcgtgatgtc tgccgcggtg atgatgtttt tgtagtgcaa
     4801 tcgttttttg atataggtgt cggtgtagtc ctcgttgatg taagtcccgg tgagccgttt
     4861 tggttttagt aaggcaagat gtgggtcggt tattggtggt acctggagtt ttttatcctg
     4921 gcgatattgt tcgaggaaac aagcgtgttt gaagttaatc acaaggattt ttccaaggtc
     4981 tttggc
//
LOCUS       DATMXJ010000212        13321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_56669, whole genome shotgun sequence.
ACCESSION   DATMXJ010000212 DATMXJ010000000
VERSION     DATMXJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 13321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13321
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56669"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VN377_05690"
     CDS             complement(<1..532)
                     /locus_tag="VN377_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27909.1"
                     /translation="MNIRFNDREKKIINCIEEITGITPTISKQPELEKEQHIEPYRIK
                     QILLLSSSYHFFQLEEEGRLSSLLQENYASHGRDSPPQITHVESQEECFSLLKNQRFD
                     LVIIFDKLDDIDSFSLATQIKSFTNIPIVLLGNNIAELIKIEEKNTEHILSKILTWNG
                     DGKIILTIIQLIEDSVN"
     gene            complement(542..1798)
                     /locus_tag="VN377_05695"
     CDS             complement(542..1798)
                     /locus_tag="VN377_05695"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWR27910.1"
                     /translation="MAQQQFDRIADQLGLDEPTRELLRNPLREYSFSIPIRMDDGNIK
                     VFRGFRVQHNDARGPCKGGIRFHPQETQDTVRALSMWMTWKCSVADIPLGGGKGGIIC
                     DPHNLSENEQERLCRGWVRQIARDIGPVRDIPAPDVMTSGQHMLWIMDEYQTIMGGQY
                     PGLITGKPVELGGSRGRTEATGYGIIYILRELLKEMKMDITKTTTSIQGFGNVARYSA
                     ELFIQYGGTVISVACWDQGENQTYSYYKKTGIKLDELIKITDRFGTIDKKQAEKLGYE
                     VRPGDDWLKQDVDILIPAALEHSITEENVSQVKPRVKIIICGANGPVTPEAENILEKK
                     GVIVIPDFLANAGGVVCSYFEQVQSNQNYYWTKEEVLGKLDNKMTDAFYKVWELSKRK
                     KIRASDAAYMIAISRVAEACKMRGWV"
     gene            2157..2600
                     /locus_tag="VN377_05700"
     CDS             2157..2600
                     /locus_tag="VN377_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27911.1"
                     /translation="MTDETGEKKEKKEGEESSSKSQLPPSEIVPEMQKGVKEKVYYRE
                     VIKSCWTHEPWLYFKIVVKSNEEMGDYIALHGLVPQNELPVHLRYKIPENEIWIRENV
                     YADVERRRSILAHEYAELDLMISQAMSYKEAHENAEFFEHLFYHE"
     gene            complement(2784..3308)
                     /locus_tag="VN377_05705"
     CDS             complement(2784..3308)
                     /locus_tag="VN377_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27912.1"
                     /translation="MKKKSVVIRKGAKEDLEVFFELYWISSLEHTHYSGVLDSLKPKE
                     KCREYIIDRQQELLKDPTQFFFVAEDEKKVVGIITGHVGERDEARIYRIERIGFVDEV
                     CVDPNYRHSGIGNRLLEAMLKQLYEEDIEFVGVGVAFKNPAFHFYKSYGFTPEGMWLI
                     QRKKSYPKKKQGKK"
     gene            complement(3410..4747)
                     /gene="gdhA"
                     /locus_tag="VN377_05710"
     CDS             complement(3410..4747)
                     /gene="gdhA"
                     /locus_tag="VN377_05710"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HWR27913.1"
                     /translation="MDKSVEEFMIKVKEKNPGEKEFHQAVQEVVESLMPYIQKNPKYK
                     KAKILERMVEPERTILFRVPWLDDKGEIQINRGYRIEMNSAIGPYKGGLRFHPSVTLS
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKTDNEVMKFCQSFMTELQRYIGPDT
                     DVPAGDIGVGGREIGFMFGQYRRLRNEFTGVLTGKGLNWGGSLIRPEATGYGATYFAQ
                     EMLKTKGESFKGKTCVVSGSGNVAQYTVEKINQLGGKVVTLSDSSGTIYDPSGIDDKK
                     LTYIMELKNVQRGRIKEYAEKYKCDYVAGKTPWHFPCDCAFPSATQNEIGKQDAKTLV
                     DNGCILVAEGANMPTAPEGVEIFIESKILYGPGKAANAGGVATSGLEMSQNSARISWS
                     REEVDKKLQWIMAQIHNSCVKYGKEGDFVNYVKGANIAGFVKVADAMIDQGLI"
     gene            complement(4881..5984)
                     /gene="thrC"
                     /locus_tag="VN377_05715"
     CDS             complement(4881..5984)
                     /gene="thrC"
                     /locus_tag="VN377_05715"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWR27914.1"
                     /translation="MKSTLRCQSCQKEYPSSEPRWHCTCGGLLTLDFHARFPIKKIKK
                     RKPTLWRYREALPIEHDQHIISFDEGFTPLIEEELFDKKVLLKQDYLFPSGSYKDRGA
                     TVLVSKIKELGIKRVVEDSSGNAGAAVAAYCAKASIDCHIYVPEKTSPEKLIQIKQYG
                     AHLHMIQGSREDTAQATWEHARTTYYASHYWNPYFFHGTKTCIFEIVEQLGWKPPDIL
                     FIPVGNGTLLNGSYIGLKELQQEDIIDKLPRIIGVQAENCAPLANAWKNNSMPIPFKT
                     PKETIAEGIAITNPARANDILHSIKETNGDIITVTEKEILEALYYIRRKGYYIEPTSA
                     VAIAGLKKLVTRKNNRVVIPLTGHGLKTKKMLR"
     gene            complement(6089..8809)
                     /locus_tag="VN377_05720"
     CDS             complement(6089..8809)
                     /locus_tag="VN377_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWR27915.1"
                     /translation="MNKRIKYVIISLIICTLCIPTALNATGNNVFSQENRKTINERWI
                     TVPDSSVVLKLWDEQLDTSEIVPFYSISLDYGKTYVRTVQPSYEIGLRYAHFDPLIGE
                     PSIEPLLTSSDDTHLFIVQFFTQPLQEFQEAITACGGAVRHYIAQYAYLVEMSDSVRT
                     EVETLPYVRWIGPYHPAYRLEEFMLDNVNNADQVYPLQHYNIQVLSVEQKKIVAEHIR
                     TIGGTINKEDAGKFLVEATLSPEQLFVVIRWDEVLFVDRWSAYEADMDIAREIGGANY
                     IESIAGYNGSGVRGEVFDAGFNLAHVDFQSRPLILHGNVGSDYHGASTSGIVFGDGTG
                     NPQARGMLPEGQGIVGDYNYIGLEGTNRYTHTGELGQDPYYGVFETSSVGSDRTTDYT
                     TISADTDSALIDFDVLHCQSQSNAGDQMSRPQAWAKNIVSGGGIYHQNTLTKTDDEWN
                     YGGSTGPATDGRIKPDLCSFYDWIFTTTGGGPTAYTDNFGGTSGATPIIAGHFGLFYQ
                     MWSDGIFGNEVDPDGTVFENRPHMTTAKAMMINTADQYPFNGTSEDKTRMHQGWGMPS
                     VKNLYDLRDNFYIIDETDVLENLDVSTHMVAVESGSPYLKVTMTYADPAGNPAVQTQH
                     RINDLTLQVISPSNVIYWGNYGLKTGVWSQPDGTADTKDTVECVFIQNPEVGTWTVKV
                     SADEIIQDSHIETPELDADYALVVSPVLSAPFPPEINGSTQGDIGRQLDFTFVTTDPG
                     NSNLYYWVEWGDGNYNEWVGPSASGAPVTISHTWSEKGNYSITAKAKNLLGVESDWSE
                     PFDLTIIAPRLEVGVVSGGLFKVKTVIRNTGAIDITQIKWNITLSGNVFIGKQTSGSL
                     LSISPGGERSISSDFILGLGKTIITVSASNPYNSVTMTQNATVLLFFIKV"
     gene            9063..10412
                     /locus_tag="VN377_05725"
     CDS             9063..10412
                     /locus_tag="VN377_05725"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWR27916.1"
                     /translation="MISTYTTKSLNKESVLAMVRENNVHFVRLQFIDILGMPKNIVIP
                     VNGLESALDEGIPFDASSIAGYATIEESDKIAKPDPASFVILPENVEERKTAKLNCDI
                     FEPNGKRFEGDVKYILEKMVDKAAALGYIYNTGPECEFFLFKKNGENSTIIPNDTAGY
                     FDLSHRDLAEGVRADISLALEAVGIQPYTAHHECADGQQEINFNYADAITTADRVITL
                     KYITKVIATKHNLHASFMPKPLYGQCGSGMHTHQSLFTLDGSNAFYDPTDKNQLSPIA
                     YQFIAGLLKYIKDLSCVLNQTVNSYKRLVPGYEAPTYIAWANRNRSALIRIPSKRGKS
                     TRCELRNPDVACNPYLAFSVMLAAGLRGIEDRLEAPAPVEKNIYALSEEERQKYGIEQ
                     LPESLGHAVAVMSKSELVRETLGDHVFENLIHVKRKEWDTYRLQVTKWEVENYLPLL"
     gene            10513..10950
                     /locus_tag="VN377_05730"
     CDS             10513..10950
                     /locus_tag="VN377_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HWR27917.1"
                     /translation="MGFEMQIVSNTPLTGEEDFDTAAKMFFEQIGYLSKGSDPLIPYK
                     IFADFFLKHPTKAWFVDDIAATLKTSRPTVYRHLNKLKGFDILEEVSVFDEVSKQQKK
                     AYRLRYGNFEKAWNFVEAHIKVAVENYGKTVEHLQRLIETKRK"
     gene            10955..11242
                     /locus_tag="VN377_05735"
     CDS             10955..11242
                     /locus_tag="VN377_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27918.1"
                     /translation="MDKKEDWKLIEGSRYKIISIGGRDSSLETEGVFRGYATIGIEEA
                     GLVIELSAEHKDTVEKLRIVPLHAILAIDVLDVKPHEGRDEEKEPSGHYYG"
     gene            11386..11676
                     /gene="albA"
                     /locus_tag="VN377_05740"
     CDS             11386..11676
                     /gene="albA"
                     /locus_tag="VN377_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HWR27919.1"
                     /translation="MEDNNIIYVGNKPPMNYVTAIMAVLNSGSFTEVVLKARGNAISR
                     AVDAAEITHNRFLTNAEVKRIDIGTEVVTNEDGRKSNVSSIEICLAMKKIEK"
     gene            11779..>13321
                     /locus_tag="VN377_05745"
     CDS             11779..>13321
                     /locus_tag="VN377_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR27920.1"
                     /translation="MKSVFDLLDPRIRELLSANNIEHPTEPQQKAIPSLLSGHHVLLI
                     APTGLGKTESALLPIFHQYLNAKESTSSDKTVKGISILYITPLRALNRDMLRRTYDWG
                     KHLGISVSVRHGDTSASERAKQARSPPDMLITTPETLQILFTGRRIRKHLSSVRWVVI
                     DEVHELASDERGAQLAVGLERLLEVTKQAGHGFQRIGLSATVGLPSEVARYLGGNEET
                     LFRPVEILEIDVTKHIDIRVEMPSVKKEDYPLAERLSIEPISVALLRRCKELIDAHTS
                     TLLFINTRDGAEILASRFHLWHDDQKIGVHHGSLSKHARIEAENDFKNGTLKALVCTS
                     SLELGIDVGDTDFVIQYNSPREVTRIIQRVGRSGHRVGQTSKGMILATNPEDLSESLV
                     IARKGLTGTLEQFLVRQNPLSVLSNQIISLALEYGRIKAKTAYDIIIRSYPFVTLSWE
                     LFQSVVSQLKDQRSIWLEDEQNDIILVKRTNSRHYFLDNISMIPDEKTYPVIDISTRK
                     SIGTLD"
BASE COUNT         3668 a         2891 c         2761 g         4001 t
ORIGIN      
        1 tattaacaga atcctctatg agttggatga tggtgagaat gatttttcca tcaccattcc
       61 atgtcaatat tttactcaaa atatgttctg tgtttttctc ttcaatttta atgagttctg
      121 cgatattgtt ccccaaaagc acaatcggga tgtttgtgaa tgattttatc tgtgttgcca
      181 aagagaaact atcaatatca tcaagtttgt caaaaataat gacaagatcg aaccgttgat
      241 ttttaagaag agaaaagcat tcctcttggc tttcaacatg tgttatctgc ggtggcgaat
      301 cacgaccgtg cgatgcatag ttctcctgta agagggaact caatctccct tcttcttcta
      361 attgaaaaaa atgatacgaa gaagaaagga gtagaatttg ttttattcga tatggttcaa
      421 tgtgttgctc tttttcaagt tcaggttgtt tggaaatagt tggggtgata cccgtgattt
      481 cttcgataca attgatgatt tttttttctc tatcgttgaa ccggatgttc atagggtcag
      541 attacaccca gcctcgcatt ttacatgctt cagcgactcg ggaaatcgca atcatgtatg
      601 cagcgtcact cgcccgtatt ttcttccgtt ttgaaagctc ccatacctta taaaacgcat
      661 ctgtcatctt attgtcgagc ttcccaagga cttcttcttt tgtccaatag tagttctggt
      721 tactttgaac ttgctcgaaa taactgcaga cgacaccacc tgcgtttgcg agaaaatcag
      781 ggatgacaat aacccctttt ttctcaagga tattctctgc ctccggggtc actggtccat
      841 ttgcaccgca gataatgatt ttcacccgtg gttttacctg actaacgttt tcttcagtaa
      901 tcgaatgttc aagagctgca ggtataagga tatctacatc ctgttttaac caatcatcac
      961 ctggtcggac ctcataacca agcttctccg cttgtttttt atcaatggtg ccgaaacgat
     1021 cggtgatttt tatgagctca tcgagtttga tccctgtttt tttatagtaa ctataggttt
     1081 ggttttctcc ctgatcccag caagctactg agatgacggt gcctccatat tggataaata
     1141 actcagctga atagcgagcg acatttccaa acccttggat tgatgtcgtt gttttcgtga
     1201 tatccatttt catttctttt aacagttcac gaaggatata gataattcca tatcctgttg
     1261 cttcggttct tccacgggac ccaccgagtt cgactggttt tccagtgatt aaaccagggt
     1321 actgtccacc cataattgtc tggtattcat ccatgatcca tagcatgtgc tgtccgcttg
     1381 tcatcacatc aggtgctgga atatctcgga ccggtccgat atcgcgagcg atctgtcgta
     1441 cccatcctct gcagagcctc tcttgttcat tttcagagag attatgggga tcacaaatga
     1501 tgccaccttt tcctccgccg agggggatat cagcgactga acatttccag gtcatccaca
     1561 tagagagtgc gcgaaccgtg tcctgggttt cttgggggtg aaagcgaatc ccgcccttac
     1621 aaggtcctcg tgcgtcatta tgttgaacac gaaatcctct aaagacctta atgttaccgt
     1681 cgtccatacg aattgggatt gaaaaactgt actctcggag tggatttcgt aatagctccc
     1741 gcgttggttc gtcaagacca agttggtctg caatcctatc aaattgttgt tgagccattt
     1801 taaaggggtt aaagccttct ttttcttttc taaaaacccc agttgttcct ttttcttgtt
     1861 ttaattcctg cttagcttgc ataattcaaa cgcctcttct tcgcccacca tgtattgtaa
     1921 tcctatataa ataatttagg aataaggaat aaaaggtaaa caagtggaca agacgtgttt
     1981 tccaatcgtt tttggaggat gtttttatat aattcatatt cgatacttcc tattggatat
     2041 cgggatgtaa caaaatctcc atcatttata aagtcgtatc aataaaaata attaaaattg
     2101 tatcgtattg atgcgagata ttacgatcga ctcttagtgc aattacggag aaagaaatga
     2161 ccgatgaaac aggtgaaaag aaagaaaaaa aggaaggcga agagagctcc tcgaaatcac
     2221 aactgcctcc ttctgaaata gtgccggaga tgcaaaaagg agtcaaagaa aaggtatact
     2281 atcgcgaagt catcaaatca tgttggacac atgaaccatg gttatatttc aaaatagtag
     2341 taaaaagcaa tgaagaaatg ggagactata tcgcattgca tggacttgtt ccacaaaatg
     2401 aactcccagt tcatctgcga tataaaatac cggaaaacga gatttggata cgggagaacg
     2461 tttatgcaga cgttgaacgc cgacgctcga ttcttgccca tgaatacgct gaactcgatt
     2521 tgatgatcag tcaagctatg agttataaag aagcgcatga aaatgctgag ttttttgaac
     2581 acctttttta tcatgagtaa ctgctttttt ccaatttttt gtactcaatg agaattttca
     2641 ccttaaaaat tagatcatct cactgtaaat atgaatcgaa aaaagaatct caacctccaa
     2701 atgattggtt tatcctttca tttagagcag tgtataaaaa ttttcgatat tcgatcgttt
     2761 acagaaatac aatgaatcaa atgttatttt tttccttgtt ttttcttcgg gtatgatttc
     2821 tttctttgga taagccacat tccttctggt gtaaaaccat aggatttgta gaaatgaaac
     2881 gctgggttct taaatgcgac accaactcct acaaactcaa tatcttcttc atagagttgc
     2941 ttcagcatcg cctccagtaa cctgtttcca atgccgctat gtcgatagtt aggatcgaca
     3001 caaacctcat cgacaaatcc aatacgttct atcctataga ttcgtgcttc atctcgttct
     3061 cccacatgtc cagtgataat tccaacaact tttttttcat cttctgcaac aaaaaaaaat
     3121 tgagttggat cttttaacaa ttcctgctga cgatcgatga tatattcccg gcatttttct
     3181 tttggtttca ggctatcgag cacaccacta taatgagtgt gttccaggga tgatatccag
     3241 tacagttcaa agaatacttc cagatcttct ttagctccct tgcgtatcac gacagatttt
     3301 ttcttcattc ctaacctttg atttctctct atccactcga caaccttgat gggtagtttt
     3361 ttcctcggaa tatagatgaa ataaataaaa aaaagaaaga aggacgatat tagatgagtc
     3421 cttgatcaat catagcatcg gcgactttga caaatcctgc gatgttcgca cctttgacat
     3481 agttgacgaa gtcaccttct tttccgtatt tcacacacga gttgtgaatt tgtgccatga
     3541 tccattgtaa ttttttgtcg acttcctctc tgctccagga gatacgagca ctattttgtg
     3601 acatctccag accactggtt gccacaccgc ctgcgtttgc tgcttttccc ggtccataaa
     3661 gaatcttact ttcgataaaa atctcgacgc cctcaggagc tgttggcata tttgctccct
     3721 ctgcgactag gatgcaaccg ttgtcaacga gtgtctttgc atcttgtttg ccaatttcat
     3781 tctgtgtcgc tgaaggaaat gcacaatcac aaggaaaatg ccaaggtgtc ttccctgcta
     3841 cgtaatcgca tttatatttc tctgcatatt ctttgatgcg tccccgttgg acatttttca
     3901 attccattat ataggtaagt tttttatcat cgattccaga aggatcatag attgttcctg
     3961 acgaatcaga taaggttaca acttttccac caagttggtt aattttttct acggtgtact
     4021 gtgcaacgtt tcctgaacct gagacgacac aggtttttcc tttgaatgac tctcctttcg
     4081 tcttaagcat ttcctgtgca aaataggtag caccgtatcc tgttgcctct ggtcgaatca
     4141 gactgccacc ccagtttagt cccttgccgg taaggacccc agtaaattca tttctcagtc
     4201 ttcggtattg tccaaacata aaaccgattt cacgaccacc aacaccaatg tctcctgcgg
     4261 ggacatctgt atccggaccg atatatcttt gtaactcggt cataaaagat tgacagaatt
     4321 tcatgacctc attatcggtt tttcctttcg gatcaaaatc agatcctcct ttcccaccac
     4381 ccatcggtag tgtcgtcaaa ctgtttttaa atacttgctc gaacgctaaa aactttaaaa
     4441 tacttaaggt gacgcttggg tgaaaacgaa gccccccttt gtaaggtcca atcgcgctat
     4501 tcatttcgat acggtaccct cgattaatct ggatttcgcc tttgtcgtcc agccagggaa
     4561 cgcgaaacaa gattgtacgt tctggttcta ccatgcgttc taaaatcttg gcttttttat
     4621 atttagggtt tttctgaatg tacggcatga gactttctac gacttcttgg actgcttgat
     4681 gaaattcttt ttcccctgga tttttttcct ttacttttat catgaattct tcgacagatt
     4741 tatccattac aatgcctccg cgttttttgt ggtttggtat cattatttcc ttttaatact
     4801 ttgcctagtc tcctttgaat aaaaacataa aaaaaatatg gagatagagc agttttttaa
     4861 taagggtcaa tatagatttt ttatcgtagc attttttttg ttttaagacc atgtcctgtg
     4921 agggggatga ccacacgatt atttttccgg gttactagtt tttttaaccc tgcgatcgcg
     4981 actgctgagg tcggttcgat ataatatcct ttccgtcgaa tgtagtacaa ggcttcaaga
     5041 atctcttttt cggttactgt gattatatca ccgtttgttt cttttatcga atggagaata
     5101 tcgtttgctc gtgcggggtt ggttatcgcg ataccttctg cgattgtctc cttcggggtt
     5161 ttaaaaggga taggcatact attgtttttc catgcatttg ctaatggtgc gcagttctcc
     5221 gcttgtactc cgataatcct cggaagcttg tcgataatgt cttcttgttg taattccttt
     5281 aaaccgatgt atgagccatt gaggagtgtt ccgttaccga ccgggatgaa aaggatatcg
     5341 ggtggtttcc atccgagttg ttcaacgatt tcgaagatac atgtttttgt cccatggaaa
     5401 aaataggggt tccaatagtg actcgcgtag tatgtcgttc gtgcgtgttc ccatgttgcc
     5461 tgtgcggtat cttcccggct cccttgtatc atatgtagat gtgctccgta ttgtttgatt
     5521 tgtatgagtt tctccgggga ggtcttttct gggacataga tgtgacaatc gatagatgct
     5581 tttgcgcaat atgctgcaac cgcagcacct gcattgccgg aagagtcctc aacgactctt
     5641 ttgattccta actcctttat cttactgact agcaccgttg ccccacgatc tttatatgat
     5701 ccgcttggga aaagatagtc ttgttttaac aggacctttt tgtcaaacaa ctcttcctca
     5761 ataagggggg tgaatccttc atcaaaagag atgatatgct gatcgtgttc gataggtagt
     5821 gcttcgcggt atcgccagag agtcggtttt ctttttttga ttttctttat tgggaatcgt
     5881 gcgtgaaaat caagagtcag taacccgccg caggtgcagt gccatctcgg ctcagacgat
     5941 gggtattctt tctgacagga ttgacaccgc aatgtacttt tcattatagt gttgattttc
     6001 agaaccctat gtaaatgttt tgctcactac caaaaaatga aagaatgaaa cacgattctc
     6061 gtgtttctaa gaggaaagat tgtacggttt acactttgat gaagaacaac agtaccgttg
     6121 cattctgtgt catggtaact gagttatagg gatttgacgc tgagactgtg ataatcgttt
     6181 taccgagtcc gagaataaaa tccgagctga tcgaccgttc tccacctggt gagatactta
     6241 acaaactacc gcttgtctgc ttaccgatga acacattccc tgagagggtg atattccatt
     6301 ttatttgtgt aatatcgatg gcacctgtgt ttctgatgac tgttttaacc ttgaatagac
     6361 caccagaaac cacacctacc tcaagtcttg gtgcgataat cgtcagatca aagggttctg
     6421 accaatcgct ttcaacccct aggaggttct tcgcttttgc agtgatcgaa taattccctt
     6481 tctcagacca ggtatgtgag atcgtgacag gagctccgga ggctgaggga ccaacccatt
     6541 cgttatagtt tccatcgccc cattcgaccc aataatacag gttcgaattc cccgggtcag
     6601 ttgtgacgaa ggtgaaatcc aactgacgtc caatgtctcc ttgggtcgat ccgttgatct
     6661 ctggtgggaa tggtgcggaa agaactgggc ttaccaccaa cgcatagtct gcatctaatt
     6721 ctggtgtttc aatgtgagaa tcctggatta tttcatctgc ggacaccttg acggtccagg
     6781 tcccgacttc agggttctga atgaagacac attcgaccgt gtctttcgta tcagctgtac
     6841 catcaggctg agaccagaca ccagtcttga ggccataatt tccccagtat atcacattgg
     6901 atggagagat aacctgcaag gttaaatcat taatacggtg ctgggtttgg accgctgggt
     6961 ttcctgcagg atctgcatag gtcatggtga cctttaggta tggtgaccct gattcaaccg
     7021 caaccatgtg ggttgataca tctaagtttt caaggacatc tgtttcatca atgatataga
     7081 aattatctcg taaatcatag aggtttttca cagatggcat cccccatcct tggtgcatac
     7141 gggttttatc ctccgatgtt ccattgaaag ggtattgatc ggcggtgtta atcatcatcg
     7201 ctttcgctgt tgtcatatga ggtcggttct caaagaccgt tccatccggg tcaacctcgt
     7261 ttccgaagat gccatcagac cacatttgat aaaataaacc gaagtgacca gcgatgatcg
     7321 gtgttgctcc gctcgtgcca ccaaagttat cagtgtaagc ggttggaccc cctccggttg
     7381 tcgtgaatat ccaatcataa aaagaacaaa gatcaggttt tattcttccg tcagtagcag
     7441 gtccagtact ccctccgtaa ttccattcgt catcggtttt tgtcagagtg ttttggtggt
     7501 aaatacctcc accagagacg atgttcttcg cccatgcctg tggccgggac atctggtcgc
     7561 cagcgttgct ttgtgattgg caatggagaa catcgaaatc tatgagtgcg gagtcggtat
     7621 ccgcggatat cgtggtgtaa tcggtcgtcc gatcgcttcc gacacttgag gtctcaaata
     7681 cgccatagta tggatcttgt cctaactctc ctgtgtgggt atatcgattc gttccctcca
     7741 acccgatata attataatcg ccgacaattc cttgaccttc aggaagcatc cctcgagcct
     7801 gtggattacc ggttccatcc ccaaagacga tccctgaggt tgaggcacca tgataatcac
     7861 ttcccacgtt cccatgtaag atcaatggac gtgattgaaa atcaacgtga gcaagattga
     7921 accctgcatc gaaaacctca cctcggacgc cgctcccgtt gtaccccgct atggattcga
     7981 tatagttcgc cccaccaatt tcccgtgcga tatccatatc ggcttcatac gcgctccatc
     8041 gatcaacaaa cagcacttca tcccaacgaa tgaccacgaa cagttgctct ggcgataagg
     8101 tggcttcgac gaggaatttc cctgcatctt ctttgtttat cgttccaccg attgtcctga
     8161 tatgctctgc aacgattttc ttttgttcta cagaaagaac ttgaatatta tagtgttgca
     8221 gagggtagac ttgatcggca ttatttacgt tatctagcat gaattcctca agccggtacg
     8281 cgggatgata cggcccaatc caacgcacat atggcaatgt ctcgacctct gttcggactg
     8341 agtcgctcat ctcgacaaga tacgcgtatt gagcgatata atgacggacc gcacctccac
     8401 aagcggtgat tgcttcttga aattcttgaa gtggctgtgt gaaaaactgg acaatgaaaa
     8461 gatgcgtatc atcacttgaa gtcaataaag gttcgatgga tggttctcct atcaaaggat
     8521 cgaaatgcgc gtaccgcaga ccaatttcgt aggacggttg gactgttctc acatacgttt
     8581 ttccatagtc cagactgatg gaataaaacg gcacgatttc acttgtatcg agttgttcgt
     8641 cccataattt caatacaact gatgaatcag gtactgtgat ccatcgttca ttaatggttt
     8701 tcctattttc ttgggaaaac acattgttcc cagtggcgtt cagagctgtc ggaatacaca
     8761 gtgtacagat gatgaggctt attattacat attttattct tttattcatt tatatccccc
     8821 ttcaaattac ttttctttac acgataatat aattatttaa tacaaagcct gtaattaaat
     8881 attcctatgt gttgtctttt atatattttt ttaaaaaata cagatgtttc ccggagaaaa
     8941 tattattttt tttggaaaaa atcggaagtt cgataagcta ttaatacata atctttatac
     9001 agactgagtg acagaaatga atcatttttg tccttaagaa tactatcaaa taggtgagga
     9061 acatgatcag tacatatacg acgaaatcgt tgaataagga atctgtccta gccatggtcc
     9121 gggaaaataa tgttcatttc gttcgtctac aatttattga cattcttgga atgcctaaga
     9181 acatcgtaat accggtaaac gggcttgaat ctgccttgga tgagggtatc ccatttgatg
     9241 catcgtcaat cgcggggtat gcaaccattg aagaatcaga taaaatcgca aaacctgatc
     9301 ctgcaagttt tgtgattctt cccgagaacg tcgaagaaag aaaaactgcg aaactcaact
     9361 gtgatatctt cgagccgaac gggaagcgat tcgaaggtga tgtgaaatat attttagaaa
     9421 aaatggtaga taaggcagcc gctcttggtt atatctataa tacaggccct gaatgtgagt
     9481 tttttttgtt taaaaaaaat ggtgagaact cgaccatcat tccaaacgat accgcagggt
     9541 actttgattt atcacatcgt gatcttgctg aaggagtccg agctgatatc tccttagcat
     9601 tagaggcggt cggaatccaa ccgtataccg ctcaccatga atgtgctgac ggtcagcaag
     9661 aaatcaactt taattatgcg gatgcgatca ccaccgctga ccgagtgatt actttgaaat
     9721 atatcacgaa ggtcattgca acaaaacata acctccatgc ttcttttatg cccaagccac
     9781 tttatggaca gtgtggttct ggtatgcata cccatcaatc gctcttcaca ttagatggtt
     9841 caaatgcatt ctatgaccct acggataaaa accaattaag tcccatagca taccaattta
     9901 tcgcaggtct cctgaaatat attaaagacc tttcttgtgt gttgaatcag acagtgaatt
     9961 cctacaaacg attagtccca gggtatgaag caccgaccta tatcgcctgg gcaaaccgga
    10021 atcgcagtgc gctgatccgt atcccatcga aacgaggtaa aagcacgcgg tgtgaacttc
    10081 gaaatcctga tgttgcctgc aatccgtatc tagcattttc agtgatgctt gcagcaggtt
    10141 tacgaggaat cgaagataga cttgaggcgc cggccccggt tgaaaagaat atctatgcat
    10201 tgtcagagga agaacgtcaa aaatacggta ttgaacagct tccggaaagc cttggccatg
    10261 ccgtggcggt gatgagtaaa agtgagttgg tacgggaaac acttggtgac catgtctttg
    10321 agaatttgat tcatgtgaag cgaaaagaat gggataccta tcgcttacag gtgaccaaat
    10381 gggaagtgga aaactatctt cctcttcttt aaagtaaagc ctgttccact accgaataat
    10441 tttttttcct tttctctgtt aaagaaacgt tattaattga cgaatgtatt cagggatgtc
    10501 gaggctgaac ctatgggttt cgaaatgcag attgtcagca acacaccgct gaccggtgaa
    10561 gaagattttg atactgccgc gaagatgttc tttgaacaaa tcgggtatct ttcaaaaggc
    10621 tctgatcctc tcatccccta taaaatattc gcggatttct tcctcaaaca cccaacaaaa
    10681 gcatggtttg ttgatgatat cgcagcgacc ttgaagacct cacgaccaac ggtctatcgg
    10741 cacctcaaca aactgaaagg ctttgatatc cttgaggaag tctcagtgtt tgatgaagtc
    10801 tcaaaacagc agaaaaaagc ctatcgattg cgctatggga attttgaaaa agcatggaat
    10861 ttcgttgaag cacatattaa agtagcagtt gagaattacg gcaagacagt cgaacatctt
    10921 caacgactca tcgaaacaaa aaggaagtga ctcaatggat aaaaaggaag actggaaact
    10981 catagaagga tcacgttata agatcatctc cataggtgga cgagacagct ccttggaaac
    11041 agaaggagtg tttcggggat atgccacgat tggcatcgaa gaagctggtc ttgtcattga
    11101 actatccgcg gaacacaaag atacagttga aaagcttcgt atcgtccctt tgcatgcgat
    11161 tttagccata gatgtccttg atgtaaaacc ccatgaagga cgagacgagg agaaagaacc
    11221 cagcggtcat tattacggat aaaacaatca aataccatcc cgatatcctc taaaaaaaca
    11281 aaacctttat taaagagaaa tgacttttca tttgccagag aacaaagaag cagaccattt
    11341 ttctggggag cgagatttgg tatgtctacg gatgagaaaa ggaaaataga agacaacaat
    11401 atcatctatg tcggaaataa gccaccgatg aattatgtca ccgccatcat ggcggtttta
    11461 aactctggga gttttaccga ggtggtgctg aaagcgagag gtaacgcaat cagcagggca
    11521 gtcgatgcag cagaaataac ccacaaccga tttttgacaa acgctgaggt aaaacgtatt
    11581 gatatcggaa ccgaggttgt gacgaatgag gatggccgaa aatcaaatgt ctcctctatt
    11641 gaaatctgtc ttgcgatgaa aaaaatcgag aaataacata gaagaaccta ctcgtactct
    11701 gttggatttc acactatgct aagtacattt tttcatagta aaaaatacta aatacaggat
    11761 gagaatgcat ccttgcccat gaaatcagtt tttgatcttc ttgacccccg tattcgagaa
    11821 ttgctctctg cgaataacat agaacatccg acagaaccac aacaaaaggc aatcccctct
    11881 cttctcagcg gacatcatgt gcttttgatc gcaccaaccg ggttgggaaa aacagaatca
    11941 gcattgctac ccatctttca tcagtatttg aatgcaaaag aatccacctc ttccgataag
    12001 accgtcaaag ggatttcgat actgtatatc actccattac gcgcattaaa ccgggacatg
    12061 ctccgtcgga catacgactg gggaaaacat cttggtatct cagtgtcggt ccgccatggg
    12121 gacacttctg cgagcgagcg agcgaaacaa gcacggtctc caccagatat gttaatcacg
    12181 actcctgaga ccctgcagat tctttttaca ggcagacgca tacggaagca tctcagcagc
    12241 gtccgctggg tcgtcatcga tgaagtccat gagttggcca gtgacgaacg tggagcccag
    12301 cttgcggtcg gattagaacg tttacttgaa gttacaaaac aggctggtca tgggttccaa
    12361 cggattggtc tatctgctac cgttggcctg ccaagcgagg tggcacgata tctcggtggg
    12421 aacgaagaaa cattgtttcg tccagtggaa atcctcgaaa tcgatgtcac caaacatatc
    12481 gatatcagag tcgaaatgcc atctgttaaa aaggaggatt atccgctcgc tgaacgatta
    12541 tccattgagc cgatatcagt tgcgctgctg cgaagatgta aggagctcat cgatgcacat
    12601 acctccacac tcttatttat caacacaagg gatggggcgg aaattcttgc ttcacggttt
    12661 cacctctggc acgatgatca gaaaatcggt gttcatcatg gttcactatc aaaacatgca
    12721 cgcatcgagg cggagaacga tttcaaaaac gggaccttga aagcattagt gtgtacttca
    12781 tctcttgagc tcggaatcga tgtgggtgat actgattttg tgattcaata caactccccc
    12841 cgggaggtca ctcgtatcat ccaacgtgtt ggtcgatccg gtcatcgagt cggtcagaca
    12901 tcaaaaggga tgattcttgc gacaaaccct gaagacctct cggaaagttt agtaattgcc
    12961 cgtaagggat taactggtac cttagaacag tttttggtac ggcaaaatcc tctctcagtc
    13021 ctctccaatc aaatcatatc gcttgcgttg gaatatgggc gaatcaaagc aaaaaccgct
    13081 tatgatatca tcatacggtc ctaccctttt gtgacgctct catgggaact ttttcaatcc
    13141 gtcgtctctc aattgaagga tcagaggtcg atatggttgg aggacgaaca aaacgacatc
    13201 atcttagtga aacgaacgaa ttcacgacat tatttcctcg ataatatttc gatgatccct
    13261 gatgaaaaaa catatccggt cattgatatt agcacaagga aatccattgg cactcttgat
    13321 g
//
LOCUS       DATMXJ010000213         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_51095, whole genome shotgun sequence.
ACCESSION   DATMXJ010000213 DATMXJ010000000
VERSION     DATMXJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51095"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="glgP"
                     /locus_tag="VN377_05750"
     CDS             complement(<1..926)
                     /gene="glgP"
                     /locus_tag="VN377_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWR27921.1"
                     /translation="METSAVEIKRETNNAIRPTGKPKIAYFSMEIGIDEHIPTYSGGL
                     GVLAGDTLKSCADLNVPIVGVTLLSEKGYFYQEIDMDGNQIELPYNFNINDFLKLLPS
                     KTCVKIEGRDVTVRVWYYPVQGTSGFIVPVFFLDTNLEENSSYDREITKYLYGGDNKY
                     RLAQEIVLGIGGVRTIDSLGYRTIDKYHMNEGHAALGTLELYDQLKDVEKVRNQCVFT
                     THTPVAAGHDQFDLPLAKSMIGELIPDSLLHEFTFENKLNMTRLALFFSHYVNGVAKK
                     HGEVSRLMFPGYAIDSITNGVHTPTWVSEPFQR"
     gene            complement(1020..1328)
                     /locus_tag="VN377_05755"
     CDS             complement(1020..1328)
                     /locus_tag="VN377_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWR27922.1"
                     /translation="MIEITIGSLEEQIIKLLRTTYPITTFEISQTLRVSRREIEWILH
                     KFQVKGIVKLEPLPDITYVRLLRNDFQFVGPKQQRKIMKHTTDAKKEESNTYEGIMYS
                     "
     gene            complement(1344..2777)
                     /gene="purF"
                     /locus_tag="VN377_05760"
     CDS             complement(1344..2777)
                     /gene="purF"
                     /locus_tag="VN377_05760"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWR27923.1"
                     /translation="MCGIIGIIGGRPVSHLIYHSLFTIQHRGQSSAGMLTYDGNIHVT
                     KDSGLVRDVFNEDKKINKPGNIGIGHTRYSTAGMDDEESLKKNAQPEYLVNPFLAAVH
                     NGNIYNCSELTNITDRKPRTDCDIQWLLLPMADELYKKELNPEIIFTACEHVMKKAKG
                     SYSVIYIADSGDKPYLFAMTDPRKVRPLALGKSNGTYCLASETRVFKKINFDYVKDIP
                     GGSVIVIDPKGTIYEKQIIKKQELPCMFEFVYFAKPDSRINGRSIHTTRQEIGRLLAQ
                     EQPAKADLVIPVPESGRRYAIGYSLESGIPLDEGIMKDKDERSFIQQTQEHREKVVEE
                     GLSFLHAVLEDKRVVLVDDSIVRGTNIRKIIQEMKNVGTKEVHVRIGCPPLIAPCYLG
                     IDMRSKKEFIARNSDGNIKAWEQIAEEIGADSLGYISDQGLKKAIGFDTCKGCIDFPD
                     GYPPEMRDDVKKLFKNDKKGMRAYECI"
     gene            2976..3944
                     /locus_tag="VN377_05765"
     CDS             2976..3944
                     /locus_tag="VN377_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWR27924.1"
                     /translation="MVKVDSKDREILYQLDLDSRQSFAKIGSKVGLSKTVVAYRVNKL
                     IENGIIKTFYTVIDAFKLGYIAFRIYLVYQYMTQEKEKEIIEYFTKQKLNWWTISAEG
                     RFDLALIMWVKDINDFYSFWEETLKQFRDYFLEQQFSVYIQLYTYRHSYLLEDIKKSD
                     RTKFEITGGGAQVMIDELDFKLLRLIAPHARMPVKEIAKRLDTTVAVVNYRIKKLIKT
                     GVIQGFRSEMDLGMLGYQFFKADIDLKDYRQRGKIINYAKTNPYLVRIDKSVGISDLE
                     LEYHVQSLEQFHEIMKDLINKFPDVIKKYKYVYASKLHKMNYMPEA"
     gene            complement(3946..>4653)
                     /locus_tag="VN377_05770"
     CDS             complement(3946..>4653)
                     /locus_tag="VN377_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27925.1"
                     /translation="SYMSGFEATKLFSSNKDTNPENTPLSQNIIREINEGCGFLFLDG
                     HGGPSWWNTNWPGEFGSLIQNGGLTIYQFSQLKNGAKLPICIVGGCHNNLFNVSLLTT
                     ITDPRNRRSMWSNGAPIPECWGWSLTVKPNGGAIATLGNTGLGYEAGGELGDLNGDGI
                     NEPDCVESLCGYLETQFFKGYQVNHIDFLGKNWCQAIGEYLRIYPGMEQWSDAKTLEQ
                     WVLFGDPSLKIGGYASS"
BASE COUNT         1175 a         1016 c         1019 g         1443 t
ORIGIN      
        1 cgttggaatg gttcagagac ccatgttggg gtatggactc cattggtgat cgaatcgatc
       61 gcgtatcctg ggaacatcaa tcgtgatact tctccatgtt tctttgccac accattcacg
      121 taatggctga aaaagagggc aagtctggtc atattgagct tgttttcaaa ggtgaactcg
      181 tgcagcaagg agtctgggat taactccccg atcattgatt ttgctaatgg taagtcgaac
      241 tgatcatggc ctgcagcgac tggtgtgtgg gtcgtaaaca cacattgatt cctgactttc
      301 tccacatcct ttagctgatc gtacaattca agtgtgccga gagcagcatg tccttcgttc
      361 atatggtatt tatcgatggt ccgataacct aatgagtcta ttgtacgcac accaccgatg
      421 cctaggacga tttcctgtgc gagacgatat ttgttatctc ctccgtagag atatttggtg
      481 atttctcggt cataggatga gttctcttca agattggtgt caaggaagaa gactgggacg
      541 ataaacccgc ttgttccttg gactggatag taccacacac gtacggtaac gtctcttccc
      601 tcgattttca cacaggtttt acttgggagc agtttgagga aatcattgat gttgaaatta
      661 tacggaagtt cgatttgatt cccatccatg tcaatttctt gataaaaata ccctttctca
      721 gacagaagtg tgactccgac aatcggtaca ttgaggtccg cgcatgattt cagggtgtcc
      781 cctgcaagaa cccctagacc gccgctgtag gttgggatat gttcatcgat cccgatttcc
      841 atggaaaaat atgcgatttt tggtttccct gtaggtctaa tggcgttatt tgtttctctt
      901 tttatttcta ccgctgaagt ctccatctat ccaccttcac tctctttaga acactcttcg
      961 aatgggatga tactcagaac gagtctaccg tttatctttt tttctctttt ggctgtgagt
     1021 taggaataca tgatgccttc gtacgtgttg ctctcctctt ttttggcatc ggttgtgtgc
     1081 ttcatgattt tgcgttgctg ttttggtcct acgaactgaa aatcattccg caatagcctg
     1141 acgtaggtta tatctggaag tggttcgagt ttgacgatgc ctttgacttg gaatttatgt
     1201 aaaatccatt cgatttctct tcgcgatact cgaagggtct gcgatatttc aaaggttgtg
     1261 atagggtagg ttgtccgcaa tagtttaatg atttgttctt ctaacgatcc aatggttatt
     1321 tctatcatac gataaccatg tagttatatg cactcgtatg cccgcatccc ttttttatcg
     1381 tttttgaaga gcttttttac atcatcacgc atttcggggg gatagccatc tgggaaatca
     1441 atgcatcctt tgcaggtgtc aaatccgatt gctttcttca gcccctgatc gctgatgtat
     1501 cctaaagaat ctgccccaat ttcttcagca atctgttccc atgctttgat gtttccatcg
     1561 ctgtttcttg cgatgaattc ttttttgctt cgcatatcga taccgagata acatggtgcg
     1621 atgagcgggg ggcatccgat cctcacatgg acttcctttg tcccaacgtt tttcatttcc
     1681 tggatgatct ttcgtatatt ggtcccgcgg acgatagaat catcgactaa aacgactcgt
     1741 ttatcttcaa ggaccgcgtg gagaaatgac agtccttcct caacgacttt ctctcgatgc
     1801 tcctgtgtct gttgtatgaa tgatcgttca tctttatcct tcatgatccc ttcatcgagt
     1861 gggatgcctg attccagtga gtacccaatg gcgtatcgtc ttcctgactc ggggactggt
     1921 atcacgaggt ctgccttggc tggttgctcc tgtgcgagta accgtccgat ctcctgacgc
     1981 gtggtatgga tcgacctgcc gtttatgcgt gagtcaggtt ttgcgaaata gacaaattca
     2041 aacatacagg ggagttcctg cttcttgatg atctgttttt cgtagatagt ccctttcggg
     2101 tcgatgacga tcaccgagcc acccgggata tctttgacat aatcaaaatt aattttttta
     2161 aacacccgtg tctctgacgc aagacaatag gttccatttg attttccaag ggcgagtggt
     2221 ctgacttttc gtgggtcggt catagcaaag agatatggtt tgtctccgga atcagcgata
     2281 tagatgaccg aatagctccc ctttgctttt ttcatgacgt gttcgcaggc agtaaaaatg
     2341 atttctgggt tcagttcttt tttgtacagt tcgtcagcca tggggagcaa gagccattga
     2401 atatcgcaat ctgttcgtgg ttttctatcg gtaatgttcg ttagttcaga gcaattgtaa
     2461 atgtttccgt tgtgtactgc tgctaaaaaa gggttgacga ggtattctgg ttgtgcgttt
     2521 tttttgagtg attcttcatc atccatgcct gcggtcgaat atcgtgtatg accgattccg
     2581 atgttccctg gtttattgat ttttttatct tcgttaaaca catctcgtac gagacctgag
     2641 tcttttgtaa catggatgtt cccatcatag gtgagcatgc ctgcggatga ttgacctcta
     2701 tgttggatgg taaataaact atgatagata agatgtgaga caggccgtcc cccgatgata
     2761 ccgatgatac cacacatgag taaaccgtga tgaaagatac cttatttata ggttttctgt
     2821 gttctcgttg tcagagaatc attttttccc cgagaaaact aatggtttct ccttcaaggt
     2881 accatggtta ttctattttc gtcttattat catctgacta ttttgaacga aataaaaaat
     2941 ttttatacta taattataat tacgaacata catttatggt aaaagttgat tcgaaagatc
     3001 gggagattct gtaccaatta gatcttgatt ctcgtcaatc ttttgctaaa atcggaagta
     3061 aagtagggct ttcaaaaaca gtcgtggcat atcgtgtcaa taaactgatt gaaaacggga
     3121 ttataaaaac cttctacacc gtcattgatg catttaaact tgggtatatc gcctttcgca
     3181 tctaccttgt gtatcaatat atgacacagg agaaagaaaa ggaaatcatc gaatatttca
     3241 cgaaacagaa gttgaactgg tggacgatct ctgctgaagg acggtttgac cttgcgttga
     3301 tcatgtgggt aaaagatatc aatgattttt actcgttctg ggaagaaacc ttgaagcagt
     3361 ttcgcgacta ttttctggaa cagcaattct cagtatacat ccagttatac acctatcgtc
     3421 attcgtacct tctcgaagat ataaaaaaat cagatcgaac aaaatttgaa atcacgggtg
     3481 gaggtgctca ggtgatgatt gacgaactgg atttcaagct cctccgattg atagcacccc
     3541 acgcgcgcat gccggtcaaa gaaatcgcga aacgactcga taccacggtc gcggtcgtga
     3601 actatcggat caagaagctc ataaaaacag gtgtcatcca ggggtttcga tctgaaatgg
     3661 atcttgggat gctcggctat caattcttca aagcagatat cgacttaaag gattatcgac
     3721 aacgaggcaa gatcatcaac tatgcaaaga ccaacccgta tctggtacgg atcgacaaat
     3781 cagtcgggat ttctgatctt gaactggagt atcacgtaca aagcttagaa caattccatg
     3841 agatcatgaa agaccttatt aacaaattcc ctgacgtgat aaagaaatac aaatacgtgt
     3901 atgcgtcaaa gctgcataaa atgaactaca tgcctgaagc gtaaactacg atgaggcgta
     3961 cccgccgatc ttcaggctgg ggtcaccgaa caacacccat tgttcaaggg tttttgcatc
     4021 actccactgc tccatccccg ggtagatgcg gaggtactca ccgatagcct gacaccaatt
     4081 tttccctaag aagtcaatat ggttcacctg gtatcctttg aagaattgtg tctcaagata
     4141 cccgcagagc gactcgacgc aatcaggctc attgattcca tctccattga ggtctccgag
     4201 ttctccacca gcctcgtacc caagacccgt gtttcctaag gtggcgatcg cccctccgtt
     4261 cggttttaca gtaaggctcc atccccagca ctcagggatc ggagctccat tggaccacat
     4321 ggagcgccta ttgcgcgggt cggttatcgt cgtcaacaga gagacattga acagattgtt
     4381 atggcaacct ccgacaatgc agataggcag tttcgctcca ttcttcaact gtgaaaattg
     4441 atagatcgtc agtcccccgt tttggatcaa ggaaccaaat tcacctggcc agttggtgtt
     4501 ccaccaggat ggccctccat gtccatcaag gaataaaaac ccgcatcctt cattgatttc
     4561 tctgatgata ttttgtgaaa gcggggtgtt ttcaggattt gtatccttgt ttgatgaaaa
     4621 caatttcgtt gcttcaaacc cggacatata gga
//
LOCUS       DATMXJ010000214         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_62097, whole genome shotgun sequence.
ACCESSION   DATMXJ010000214 DATMXJ010000000
VERSION     DATMXJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62097"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="mnhG"
                     /locus_tag="VN377_05775"
     CDS             complement(<1..310)
                     /gene="mnhG"
                     /locus_tag="VN377_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HWR27926.1"
                     /translation="MILDILIFVFIGIGVFFNLLAGIGLLRFPDVYTRLHAGTKCTTF
                     GSIFLIGSVVLIGLNMWWEKDTNGSVLAIHSAVALIAILLTNPVGAHAIARAAHRSGV
                     Q"
     gene            complement(310..579)
                     /locus_tag="VN377_05780"
     CDS             complement(310..579)
                     /locus_tag="VN377_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWR27927.1"
                     /translation="MIDLNTTFLLVVIALILCIILALIRVFRGPTTPDRAVGVDTINT
                     MVIVSMVAYGAAFQEVIYIDVAIVYALLSFISTLFIAKYLEGGEF"
     gene            complement(576..1100)
                     /locus_tag="VN377_05785"
     CDS             complement(576..1100)
                     /locus_tag="VN377_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HWR27928.1"
                     /translation="MRAFVITTIVCFLIYLLLTTGSGSDFLGLWSFIELVFGILLSLI
                     VGFIARTVFVKNNYRLLNPVRWVLFVAYLIGPFFFSLAKANLDVAYRVITGKINPGIV
                     RISPGLTTDLGITMLANSITLTPGTLSVDIDEDTNDLYIHWINVDKKALEKKPVDCSY
                     ICGSFPQWIRRIVE"
     gene            1261..1923
                     /gene="thyX"
                     /locus_tag="VN377_05790"
     CDS             1261..1923
                     /gene="thyX"
                     /locus_tag="VN377_05790"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015505485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWR27929.1"
                     /translation="MHVKLIGYTSDPEKLPAMAATLTHSKTKPEDLQDLPIKKQTAIL
                     EQVLKLGHTSVIEHASFTFAISDVSRSLTHQLVRHRIASYSQQSQRYVNLGEPNYVIP
                     PLISKNKKTKNAYEKTMQVIWEHYNKLLELDIPAEDARYVLPNATCTNIMVTMNARSL
                     HNFFELRCCLHAQWEIRLLANKMLQEVKKVAPTIFQNSGPTCKTKGFCPEKKKDCPLY
                     PP"
     gene            1930..2580
                     /locus_tag="VN377_05795"
     CDS             1930..2580
                     /locus_tag="VN377_05795"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HWR27930.1"
                     /translation="MKRPRVTINCAMSADGKTASPTRKQMRISSDEDILRMYRLRNES
                     DAVLVGIGTILADDPKLTVKETYVQHPKQPLRVILDSKGRTPPDALALNTVSKTLIIT
                     AKGQKKTYEGAHIEVAECTTDRDGVIDVNCALDLLYQKGVRKLLVEGGGTVIWKFLKN
                     KVVDDLYIFIGSCIIGGKDTPTVADGEGVSPGEEIALKMIEMKQVGSGVLIHYQLA"
     gene            3032..>4302
                     /locus_tag="VN377_05800"
     CDS             3032..>4302
                     /locus_tag="VN377_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR27931.1"
                     /translation="MKISVFLIIGILFSSSVLVTGLSNEEHTQTSTLSLSFHEPSLIA
                     NESSFTVLMDGAPACMYQPGKPVLPMYTTTLQLPFGSVIKDVAIHFEDVHTLQLDEKI
                     TLAPHPFTQGTFRKPSVQTMTEEYGTTDLYPFEWTAYFTGGGLNQNSEHVTFLTVRVF
                     PVRYNQAADTLLYLDSCDITITYEPPASSPFPSHAKNDLVIICPLQFALPLLPLVEHK
                     KMVGVHTRLITLAEIYRSYPGNDRPEQIKYFIKDAVETWGTTYVLLVGGLKSHLAGKP
                     RDDINTGIRDWHLPVRYTNLWDDDVIHDPGFISDLYYADIYDGQGKFCSWDSFGDGVY
                     GGWSHSATRESPDYPLDVIDFYPDVYLGRLPCRNTAEVHSIVKKIINYEKTSADPSWF
                     KKMVVVGGDPYDDQGTNYLEGELIGEKAVSY"
BASE COUNT         1279 a         1004 c          984 g         1035 t
ORIGIN      
        1 gttgcacacc actgcggtgg gcagcacgcg caatcgcatg cgctccaacc ggatttgtca
       61 gaagaatcgc tatcaatgcg accgctgaat gaatcgccag caccgaacca ttggtatcct
      121 tctcccacca catgttcaaa ccgatgagca cgaccgaacc aataaggaag atggagccaa
      181 acgtcgtaca tttcgttccc gcgtgcaacc gcgtatacac atcagggaac cgaagcagcc
      241 caatgcctgc aagcagattg aagaacacac cgatcccgat gaacacgaag ataaggatgt
      301 caagaatcat cagaactcgc ccccctcaag atacttcgcg ataaacaagg tcgatatgaa
      361 agacaggagc gcatagacaa tagccacatc aatgtagatg acctcctgaa aagcggcacc
      421 atacgcgacc atactgacaa tcaccatcgt gttgatggta tcgaccccga ctgcacggtc
      481 aggtgtggtc ggtccgcgga acacccggat cagcgcaagg atgatgcaga ggatgagggc
      541 gatgaccacg agtaaaaacg tcgtgttcag atcgatcatt caacaatcct cctgatccat
      601 tgtggaaaac ttccgcagat ataagagcaa tcaaccggtt ttttctcaag tgctttttta
      661 tcaacattaa tccaatgaat gtacaggtcg ttggtgtcct catcgatatc gacacttaag
      721 gtccccgggg tgagcgtgat ggagttcgca agcatggtga taccaaggtc ggtcgtaaga
      781 cccggggaga ttctcacgat accagggttg atcttcccgg tgatgacccg gtatgcgaca
      841 tcaaggtttg cttttgccag ggagaagaaa aacggtccga tgagatacgc gacgaaaaga
      901 acccagcgga ctggattgag caggcgataa ttgtttttta caaaaacagt tctggcgatg
      961 aacccgacta tcaaggaaag cagaatccca aagaccagtt ctataaaact ccacagtccg
     1021 aggaaatctg aaccgctgcc ggttgtcaac aggagataga taagaaaaca tacaatggtc
     1081 gtaatcacaa atgccctcat cgggtacccc cacaccaatt ctgttatcgt ccatatggta
     1141 acattgactg ttgttaaaaa cttacggtcc atgaaaagaa aaaaaatgtg ctgacaagaa
     1201 aaaaatagta acatcaaaac acttatacag ttcatgagat aggggatatg ggaaacctgt
     1261 atgcacgtga aactgattgg gtatacctca gatccggaaa agctaccagc gatggctgcc
     1321 accttgaccc acagcaaaac aaaaccagag gatcttcagg acctaccgat aaaaaaacag
     1381 acagcgattt tagaacaggt gttgaaactt gggcatacca gcgtcatcga acatgcctcg
     1441 tttacgtttg cgatcagcga tgtgagcagg tcgctgaccc atcagctcgt gcggcatcgt
     1501 atcgcgagtt actctcagca aagccagcgc tatgtcaacc tcggtgagcc aaactatgtg
     1561 atcccgccgt tgatatccaa gaacaagaaa acaaagaatg cttatgaaaa aaccatgcag
     1621 gttatctggg agcattataa caaattactt gagctggaca ttccagcgga agacgccagg
     1681 tacgtccttc cgaacgcgac ctgtacgaat atcatggtga cgatgaacgc acggtcgctg
     1741 cataatttct ttgagctgcg ttgttgtctt catgcgcaat gggaaatccg cctgcttgca
     1801 aataagatgc ttcaagaggt aaagaaagtc gctccgacga tttttcaaaa ttctggcccc
     1861 acgtgtaaaa caaaggggtt ctgccctgag aagaaaaaag attgcccact gtatccaccg
     1921 tgagatgaaa tgaaacgacc ccgtgtcacc atcaattgtg cgatgtcggc ggatggaaaa
     1981 accgcctcac cgacacgaaa acaaatgagg atctcttctg acgaagatat cctgcggatg
     2041 tatcgactgc gcaatgaatc tgatgcggtg ctggttggga ttggtacgat tcttgcagat
     2101 gacccgaaac tcaccgtgaa agaaacgtat gttcaacatc cgaaacagcc gttacgggta
     2161 atccttgata gcaaaggaag gaccccacct gatgcgcttg cattaaatac ggtgtcaaaa
     2221 accttgatta ttacggcaaa aggacagaag aaaacatatg aaggagctca tatcgaggtt
     2281 gcggaatgta cgaccgatag agatggagtc atcgatgtga actgtgcgtt ggatcttctc
     2341 tatcaaaagg gtgttcggaa gcttctcgtg gagggaggcg gtacggtcat ctggaagttc
     2401 cttaaaaaca aagtggtaga tgacctctat atcttcatcg gatcctgtat tatcgggggc
     2461 aaggacacac cgacagtggc tgatggtgaa ggggtcagcc cgggggagga aatagctttg
     2521 aagatgattg agatgaaaca agtcggttct ggtgtgctga tccattacca gctcgcataa
     2581 ctcacaccaa tttattttaa tgttcgctgt taaaatttga acgaaatggt tttttaatag
     2641 caaattttag gcatttgacc aaatttccct acataacctt tattattcaa ggaatccctt
     2701 ccaacatcat ggggaattta tgaaattgtg gcaccaacgg gggtaagacg ataaaaattt
     2761 tgaccgactc tggattggat tgtgggaaaa cgtttgtctc gcgaaagaca aaactgaagt
     2821 attttaagtg ttcattttcc ccattcctcc tccttctcct tatcagttgg gatatcagcc
     2881 ttagataccc aaaactgagt tattctatat ccaggtcgta ttcgcttcct taacaaatca
     2941 accaaatcgt gttaccctcg ccgtgtcata accatcaata ggaaattata tatggagaga
     3001 ccccatgaca ttttcctgag gaaaaggagt catgaagata agtgtgtttt taatcatagg
     3061 aatcctgttc tcgagcagcg ttctcgtcac tggcctatca aatgaagaac acacgcagac
     3121 gtctaccttg tccctttcat tccatgaacc ctccctcatt gcgaatgaat caagctttac
     3181 cgtgcttatg gacggagcgc cagcatgtat gtaccaacca ggaaaacctg ttcttccaat
     3241 gtacaccaca acactccaac ttccgtttgg atcagtaatt aaagatgtgg caatccattt
     3301 tgaagacgta cacacgctac agttagatga gaaaatcacc ttagctccgc atccttttac
     3361 ccaaggaacc tttcggaaac cttcggttca aacaatgaca gaagagtacg gaaccaccga
     3421 tttgtaccct ttcgaatgga ccgcctattt tacaggtggc ggattgaatc aaaacagcga
     3481 acatgtgaca tttttgacag tgcgggtatt cccagttcga tacaaccaag cagccgatac
     3541 gttactgtac cttgactcct gcgatatcac tattacctat gaaccaccag catcatcccc
     3601 gtttccgagt cacgcgaaaa acgatcttgt cattatctgt cctttacaat ttgctctgcc
     3661 gctactccca ctagtggaac ataagaaaat ggttggtgtt cacacccggc taatcacctt
     3721 agcagagatt taccgatcct atcctggaaa tgaccggcca gaacaaatca agtatttcat
     3781 caaggatgcc gtagagacct ggggaacgac ctacgtgttg ctggtcggtg gtctaaaatc
     3841 acatcttgct ggcaaaccac gggatgatat aaacacggga attcgcgatt ggcatcttcc
     3901 cgtacgatat acgaatcttt gggacgatga tgtcatccac gacccagggt ttatcagcga
     3961 tctgtattat gctgatattt atgatggcca agggaagttt tgttcatggg attcttttgg
     4021 cgatggggtg tatggcggat ggtcacattc cgctacacgt gagtctccgg actaccctct
     4081 tgatgtaatt gatttttatc cggacgtcta tctaggaaga ctcccctgcc ggaataccgc
     4141 tgaagtacac agcattgtaa aaaagattat caactatgaa aaaacatcag cggacccctc
     4201 gtggtttaag aaaatggtgg tcgtcggagg agacccctac gatgaccagg ggacgaatta
     4261 tctggaagga gaactgatcg gtgaaaaagc agtctcctat at
//
LOCUS       DATMXJ010000215        10653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_9114, whole genome shotgun sequence.
ACCESSION   DATMXJ010000215 DATMXJ010000000
VERSION     DATMXJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9114"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="VN377_05805"
     CDS             <1..2111
                     /locus_tag="VN377_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27932.1"
                     /translation="LQLGMTDFASMLNTGESKQFSISMQNKDDTNVDLRFIRTKIMNN
                     TEKVVETKCNIITTCDTSKEYEVSVEIRFPFVMLEGGQPSEDNNPYFATKVGYQSHTG
                     QGGTNHINTTVYIGRENISDPRVFRLSIKPENIESGSKLTFFTSYLTVDDTGNELFQR
                     TYSIAFEPATELTITSIPREAKISYEFGRSAGVQTKISLRAEGGILDDIIQSIMLDPL
                     PSYMTFDLTILGSREFLYESDRSYNISYSIDSIQNGNLVTFNVGGLPQRISASWGLSL
                     GTLGDLAVSSFAELDMSQDVRRLSLSFFGNDHPFISLENFPRKLRFESGINLLDGTGN
                     ITLLRGLDDVRILNISLQHKDLTVTKSFELKNNFVQMKWKIDLINNSGFFNITRDSES
                     VMTFSTIIEYHNWTFTKALELRNSYLEISWDVDRQQRTGQVILSRDSGSGSPTLSFSI
                     GHDGWILDDTLEFRNNYLELFWQLPTQTNSHVELGLITSGGQLFHNTIAVVDEGTELF
                     HLGFGIQTDDQYIMSWDYLNGQISNFEWSGRLLRLTDVDIGVNLAGEVFTISADIDVG
                     QSGSIELQFNKAVAVTFVNTATPTFKIQGNVCINATRRLQLSWELGDTGHFTVYTFGQ
                     PLGDQFSLEVGYDPQHTGSYQYGFRLLGEHFIEITRTIQWYSVNGSLARIWILGDEPI
                     PGDWTLEVLWNGQWYTVPWG"
     gene            2121..5177
                     /locus_tag="VN377_05810"
     CDS             2121..5177
                     /locus_tag="VN377_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27933.1"
                     /translation="MGEKKTKIGLCILTMHLLLLTTLGQGMLQPMDIQNTFEPNGGVR
                     SGSIEVWQDEFFNGSKIDQTLSTNIVINTTIGTISMQNTYPAWIDPTFTRMKPITVYN
                     SGQETFQDYDVNVTVLYDTDMQNDFDDLRFTDMIGTQLSYYQCNIVNGVTCKVFIKIP
                     NLPPGQTTIYMFYGNPTVNDQSSFSSIFSWQDRTHPDTMVSFKAANEGAWDPDVIYGV
                     DRFLVTWEERLGPEDIDLPLPNYERTVPCVIHGRTYDKDGENPIPDNNTDIDVSIPGD
                     PSYHAENPCNAFGAGKYFVVWEENPANQPIYRYDADIKGALLYPNGTVQMRFSICTAT
                     NGQYDPQVAYDSQSNRYLVIWADARNGYDDYDIRGRLYNSLGYPVGTDFPIAYEINYQ
                     GDPWICSDNEGHFFIVFEDGIDASLGPFNLYAYRYDSNGNRLGSRITIAIGSGTVDYM
                     FPAVSYNSKSERYCVTWNDGDVSVDPTSRESYNGNIWGKIYDKTGVVIKNNYIIEPGT
                     SFIRSVSVPYFDTMFFIAYDGTTLGNQDIYGRLIAGNGTVMTGRQELSDGSSQNVDWN
                     DLAVGAGRVFATWEDERDVVSQYADVFQYVWRSVQSVGSMNVSSTVESEKELITEAQL
                     MSIAIQPEMFREWRQFFSIDTLPPSCSIVFDIMDQNATLVLKADVQDGQNLSDVNTSV
                     IRLRGTLTRSSAQTSPVLDKWNISAFVGKDIYAPTTDITLSPDEPDGNNGWYVSAVMA
                     TFMVSDVDSDPENITTYYDINGFGVEVYDPDNPPMISTERPNNYIEYWSNDSINEELP
                     HHQVEGIKIDTTEPMVSLSRPPALIPEGLVTINGSATEYTSGSGIDRVIIKLNEEVVY
                     DVVFNGESHIWFELNFIADRGETYDIHVEVWDKAGNSIEERKTVLCPDYGIYDTGYIY
                     WFNNPKIGPRQFLVTLGLSIAVSNDTLYVILPGVTSEAASVKFVATQTFLKKEYTFED
                     TDLSDGCSANLLVPFGIYKIKAYAYDETNTLLEEYTIIPKMLILLL"
     gene            complement(5195..5267)
                     /locus_tag="VN377_05815"
     tRNA            complement(5195..5267)
                     /locus_tag="VN377_05815"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5231..5233),aa:Asp,seq:gtc)
     gene            complement(5385..6071)
                     /locus_tag="VN377_05820"
     CDS             complement(5385..6071)
                     /locus_tag="VN377_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27934.1"
                     /translation="MAREFTRNMFVMLVSIMAGVIIITYFIGDILSQSRIDTMNLQHV
                     TEIGDINSRNENFTNYYLQGAVKMDSAREVREVANYYFDFALFWFNSALANQSSNLTT
                     FCIDNCTNAMERFLAAHENFGKSTPYFINARNYTNNSRYLEVLGYYIGFAGAGQNITM
                     LRYNASYYLRCAAENLSFGNMGLVESLMENFTLIEQAYQEAVQKYQQYRYQIDGYIFF
                     STIREIPDIQ"
     gene            complement(6087..8054)
                     /locus_tag="VN377_05825"
     CDS             complement(6087..8054)
                     /locus_tag="VN377_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HWR27935.1"
                     /translation="MNKKIPTVGIIFLQVILAFVAITPNSVQAGSYDGHDLAIAMLAD
                     PSTLISSQYWDRDTAGHRQSMVLSSRGNLIPTDGSSFAVISTGRADLVYATSNGLCPG
                     SERGNWFQGGQYGTPRDEANLQMQLEVPPYMHYLYYDIQFFTVEYPDYVGTQYNDQLT
                     ITVNSPSKGVSSYIINVNGGDFVLNARDTALLGTGYNLFAQDNAPSGVDWLQSTPNSN
                     GADAGASALIGREHPVSPGEIITLIIDLKDEGDNQFDSMAFFDNLRFSGYARTQIMAR
                     KTVIDLNGNLVECEDILEYSITISNIGTANQNNNPGPEFEDVIPANTQYVPGSATAGS
                     GTISYDDGTKKILWDGSIPAQSSVALSYMVTINAGLVNNTKISNQGIVHWDENEDGIN
                     DKNELTDDPTVDDGIDQDGDGLTGDDDPTIVNIWSFEAPTILTEDFAGVEDIVGGKAQ
                     NSYHGQLWFDTSAQSAESNFEVSPSYHYMTARSFKTKLRSVNDIQYWNYSFSSFNRQI
                     NTWDVWFTCGNSSEPADLYLNFTTTGGDDITKIKLEYIFVANIPLSCNYQVKMSVKTP
                     TGWVQLHSDYENGYLYNGWYKLRIEKNGNTAMNYSLMRNGVGVTDLISCQGLGSPFSS
                     LAQVKWCSTLNPVVSPIVFWDEHTVSLISIS"
     gene            complement(8058..10403)
                     /locus_tag="VN377_05830"
     CDS             complement(8058..10403)
                     /locus_tag="VN377_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWR27936.1"
                     /translation="MKNVSFSNNSYAASEVIGAIMLVLIAIAAFGVIYYQFLPVPLPS
                     PDPHVKLAGYVTDDGRVVLQHVGGEELTTYTIRVEQSDGPRTYRYDNDPWVMGQSYYL
                     PINDTLFSQEKQVKVSVFTMLSDGSTKMIFDGIITPRDNPPGPGTSPVLDPMLVTTLR
                     TRTIDEDLICYDYTIEPNIDPVTFIYHWMVASTGPYSSLTRVHLPFDSQNPFQTKDYS
                     GNNYNGTINGSTWTDQGKLGGAYQFNGDDFISIPYCFEDNYIDNVTVEAWVKTSQNSG
                     TILSYNRNKYWELAVTDGFVKWSTNSSDGAIDLTGAVPVNDNTWHLIAASYDASLGDS
                     AIYVDGRLDTYQHTHAAGKLLGSGETPVGAVGKGTGINGRQTIFSTGFEAQDEKNEWI
                     ENNATLGQQQTWMNLRYDTFETGWGNYVPGSNGSSADSYRSSAYKHEGAASACIRDNS
                     GTSSAITLNPIDIDTPAYKSIKVDFWWMWRGNGWASGEDWWLLYYNGTAWVSILDTNY
                     PSIYTKGVWYHAIIYINESNNRFPTNMRLRFQCDASYDDDLVYIDQIYINATSYARIE
                     CDFGLLPSTALTPHNGLYSIGGSGDFDPEYAAFNRTGIDVSGYSNIEISLWYSYKNTD
                     NNDFIGLYYRNGNQWKPIFEITNPLMPGGQRSWAQVIFSLPPSINILQLQFKWRTSAA
                     NKYMAIDDLEITGIRLAGEMNFTGIIDEVKIYPRVLTTEQLYQNYLCTKDGTTACSVI
                     VSEETHLDESWKCLVIPNDGMYDDVVAESNIIFIINYSGGG"
BASE COUNT         2946 a         2414 c         2387 g         2906 t
ORIGIN      
        1 cgttacagct tggtatgacc gattttgcct caatgttaaa cacgggtgaa tcgaaacagt
       61 ttagcatttc tatgcaaaat aaagatgata caaacgtcga tttacggttt atccgcacga
      121 aaatcatgaa caacaccgag aaagtcgtcg aaacgaaatg caatatcatc acaacatgcg
      181 atacctcaaa agaatacgaa gtctcagtcg aaatacggtt ccccttcgtc atgcttgaag
      241 gaggccaacc ctcagaggac aataatcctt atttcgcgac taaggtggga tatcaatccc
      301 acactggaca aggtggaaca aaccacatca acacaaccgt gtatatcggc agagaaaaca
      361 ttagtgaccc tcgtgtcttc cgactatcta taaaaccaga aaacattgaa agcggatcga
      421 aactcacctt tttcacaagt tacctcaccg ttgatgacac cggaaatgaa ctcttccaac
      481 gtacctattc catcgcattt gaaccagcaa ccgaactgac aatcacctcg atcccccgcg
      541 aagcaaaaat cagctatgag tttgggagaa gcgcgggggt tcagacaaag atatcgctgc
      601 gtgcagaagg cggaatcctt gatgacatca ttcaaagtat catgcttgat cccttaccaa
      661 gctatatgac ttttgattta acgattctcg gatcacggga gtttctttat gaaagcgatc
      721 ggtcgtacaa catttcatat tcgatagatt cgatacaaaa cgggaatctt gtcactttta
      781 atgtgggagg tttgcctcag cggatctctg cctcatgggg tctttccctt gggacacttg
      841 gggatcttgc tgtatcaagt tttgctgagc tggacatgtc gcaggatgtc agacgtcttt
      901 cgttatcgtt ctttggaaac gaccatccct ttatctcttt ggaaaatttc ccaagaaaac
      961 tgagatttga aagtgggata aatctgctgg atggaaccgg gaacatcacc ctactacgag
     1021 gacttgatga tgtcagaata ttgaacattt ccctgcagca taaagacctg acggtgacaa
     1081 aatcgttcga gttgaaaaat aattttgtgc agatgaaatg gaaaatcgat ctcatcaata
     1141 acagcgggtt tttcaatatt acccgggact ctgaaagcgt catgactttc tctacgatca
     1201 tcgaatatca caattggaca tttaccaagg cacttgagct tcggaacagc tatttagaga
     1261 tttcctggga tgttgacaga cagcaacgga ccggacaggt gatcctttcg agggatagcg
     1321 ggagtggaag tccgacactt tcgttctcaa tcggccatga cggatggatc ctggatgata
     1381 ccctggaatt tagaaacaac taccttgaac tattctggca gctgccgaca cagaccaata
     1441 gccatgtcga acttggtctg atcacctcag gagggcagtt gttccataac acgattgcag
     1501 tggttgacga gggtaccgag ttattccatc ttggttttgg catccaaacc gatgatcaat
     1561 atatcatgtc atgggattat ctaaacgggc aaatttcgaa cttcgaatgg tccggtagac
     1621 ttctccgact caccgacgtc gatatcgggg tgaacctggc aggagaagta tttaccatca
     1681 gtgcagacat cgatgttgga caatcaggtt ccatcgaact gcaattcaat aaagcagtcg
     1741 cagtaacctt cgtgaatacc gcaactccga cattcaaaat ccaggggaat gtctgtataa
     1801 acgcaacacg ccgactccaa ctttcctggg agcttggaga tacaggacat ttcaccgtgt
     1861 ataccttcgg acaaccatta ggtgaccaat tcagccttga ggtcggatat gatccgcaac
     1921 acaccgggag ttaccagtat ggattccggt tactggggga acatttcatt gagatcacca
     1981 gaaccatcca atggtattca gtcaacggct ctctcgcccg catctggata cttggtgatg
     2041 aaccaatacc aggggactgg acattggaag tactctggaa cgggcaatgg tacaccgtac
     2101 catggggtta ggtaatctat atgggggaga aaaaaaccaa gataggcctc tgtatcctaa
     2161 cgatgcatct cctgctcctt accacattgg gacagggtat gctgcaacct atggatatac
     2221 aaaatacttt tgaacccaat ggaggtgtcc ggagcggatc catcgaagtc tggcaggacg
     2281 agtttttcaa tggaagtaag atcgatcaga cgctctctac caatattgtt atcaatacaa
     2341 ctatagggac gatctcgatg caaaacacct atcctgcatg gatagatcca accttcacac
     2401 gcatgaaacc aattactgtg tataacagcg gacaagaaac attccaggac tacgatgtca
     2461 acgtcaccgt gctgtacgac acagatatgc aaaatgattt tgatgatctt cgcttcactg
     2521 atatgattgg aactcagctg tcgtattatc aatgcaatat cgtcaatgga gtcacctgta
     2581 aggtctttat caagattcca aacctgcccc cgggacaaac cacgatctat atgttctatg
     2641 ggaatccaac cgtgaacgat caaagtagtt tttcatcgat attttcctgg caggatagaa
     2701 cacatccaga taccatggtt tctttcaaag cagcgaacga gggtgcctgg gatccggatg
     2761 tcatctatgg tgtcgataga ttccttgtta cttgggagga acgactcggg cctgaggata
     2821 tcgacctccc tctaccgaat tatgaacgga cggtcccctg tgtgattcat gggaggacct
     2881 atgacaaaga tggtgaaaat cctatccctg ataacaacac agacatcgac gtgtcgattc
     2941 caggggatcc atcttatcat gctgaaaacc cctgcaacgc gtttggtgcg gggaagtatt
     3001 ttgtcgtctg ggaggagaac cctgcgaatc aaccaatcta tcgctatgat gcagatatta
     3061 aaggagcttt attgtaccca aacggaacag tccaaatgcg tttttcaatt tgtactgcca
     3121 ccaacggtca atatgatccc caggtcgcct atgatagcca aagcaaccga tacttagtta
     3181 tctgggcgga tgcacggaat gggtacgatg attatgatat ccggggaaga ttgtataatt
     3241 ctcttggata tccagtcgga acagattttc cgatagccta tgaaatcaat tatcagggag
     3301 acccgtggat atgttctgat aacgaaggac atttttttat tgtattcgaa gatggcattg
     3361 atgcttctct tggtcccttc aatctctatg cataccggta tgactcaaac gggaaccgtc
     3421 tcggctcacg aatcacgatt gcaataggca gcgggactgt ggattatatg ttccctgccg
     3481 tttcgtataa ttccaaatcg gagcgttatt gtgtcacctg gaacgatggg gatgtgagtg
     3541 tcgacccgac gagccgcgaa agctataatg ggaacatctg gggaaaaatc tatgacaaga
     3601 ctggtgtggt cattaaaaac aattacatca tcgaaccagg gacgagtttc attcgaagcg
     3661 tctcggtacc atattttgac acgatgtttt tcatcgccta tgatggaacg accctgggga
     3721 accaggatat ctatgggcgg ttgatagctg gaaacggaac ggtaatgaca ggaagacagg
     3781 agctttctga tggctcctcg cagaatgttg attggaatga tcttgcggta ggggcaggac
     3841 gggtgtttgc gacctgggaa gacgaacggg atgtcgtgag tcaatacgct gatgtttttc
     3901 agtatgtctg gcgttccgtc cagtctgttg gttccatgaa tgtttcaagt actgtcgaat
     3961 ctgaaaaaga gctcatcact gaggcacaac tgatgtctat cgctatccaa ccagagatgt
     4021 tccgcgaatg gcgacagttc ttctccatcg acacccttcc gccatcctgt tcgattgtgt
     4081 ttgacatcat ggatcagaat gcaacgttgg tcctcaaagc cgacgtccag gatggtcaga
     4141 acctctcaga tgtgaacacg tcagtgatcc ggttacgggg gacattaacg cgttcatcag
     4201 cacaaacgag cccggttctc gacaaatgga atatctctgc gttcgttgga aaagacatct
     4261 atgctccgac gaccgacatc accctcagtc cagatgaacc agatggcaat aacggctggt
     4321 acgtgtcagc ggtcatggcg acgtttatgg tctctgatgt ggatagcgac ccggagaaca
     4381 tcaccacgta ttatgatatc aacgggtttg gggtggaggt ctacgaccca gacaacccac
     4441 cgatgatttc aacggaacga cccaataact atatcgaata ctggtctaat gactcgatca
     4501 atgaggagct cccgcatcat caagtcgagg gaatcaagat tgacaccacc gaaccaatgg
     4561 tatccttgag cagaccaccc gctcttatcc cagaaggcct cgtcaccatt aatggctctg
     4621 cgaccgaata tacctctggc tccggaattg accgggtgat cataaaactc aatgaggagg
     4681 tcgtgtacga tgtggtcttc aacggggaat cacacatctg gtttgaattg aacttcatcg
     4741 cggatcgtgg tgaaacctat gacatccatg tcgaggtatg ggacaaggcg ggaaacagca
     4801 ttgaggaacg aaaaaccgtc ctctgccctg attatgggat ctatgacaca ggatacatct
     4861 actggttcaa caacccaaaa ataggaccaa gacaattcct ggtcaccctt gggttatcta
     4921 tcgccgtgtc caatgataca ctctatgtca ttctcccggg agtcaccagt gaagcagcgt
     4981 ctgtcaaatt cgtcgcaacc caaacattcc tcaaaaagga gtataccttc gaagacacgg
     5041 acctttccga tggctgctcc gcgaacctgc tggttccttt tggaatctac aaaataaagg
     5101 catacgcgta cgatgagaca aacaccttgc tagaggaata cacgattatt ccgaagatgc
     5161 tgatcctcct cttatgatat aaaaaaagga taagcgcccc ggctgggatt cgaacccaag
     5221 tcgaagcctc gacagggctt catgataggc cactacacta ccggggcagg gtatctcgtt
     5281 tgtttgtagc tgggggtaat catttctctt tctttaattt tttggaactg ctaaagcaag
     5341 aaaaaaagga agaaaatact tcatgaggcc aaaaaaaatt aagattattg gatatcggga
     5401 atctccctga tggtgctaaa gaagatatac ccatcaatct ggtaacgata ctgctgatat
     5461 ttctgtaccg cctcttggta cgcttgttcg atcaacgtga agttctccat gagcgattcg
     5521 acaagcccca tgttgccgaa ggaaaggttt tccgctgcgc atctgagata atagcttgca
     5581 ttgtaccgta gcatcgtgat gttttgacct gcaccagcaa aaccaatgta ataccccaag
     5641 acctcaagat atcgagagtt atttgtgtaa ttccgtgcgt ttataaaata cggtgtagat
     5701 ttcccgaagt tctcatgagc tgcgaggaat ctttccatgg cgtttgtaca attatcaata
     5761 cagaaggtcg taagattcga actttgatta gctaacgcag aattaaacca gaacaacgca
     5821 aaatcaaaat aataattagc gacctcccgt acttcacgtg ctgaatccat ttttacagca
     5881 ccttgtagat agtaattggt aaaattctca tttctgctat taatgtcacc gatctccgtc
     5941 acatgttgca ggttcatggt atctattcgt gattgactta agatatcacc gatgaaatac
     6001 gtgatgatga taacaccagc catgatcgag accagcataa caaacatatt tcgtgtaaat
     6061 tctcgcgcca tatttttttc acctctttat gaaatcgaaa tgagactcac ggtatgttca
     6121 tcccagaaaa caatcgggct gactaccggg tttagtgtag aacaccattt aacttgtgca
     6181 aggctgctga acggtgaacc aagaccctga caggaaatga gatctgtgac tcctactcca
     6241 ttcctcatga gcgaataatt catagcagtg tttccgtttt tctcaatacg taacttatac
     6301 catccgttgt acaaataccc attttcataa tcggagtgca actgaaccca cccagttggt
     6361 gtttttacac tcatttttac ttgataatta caagatagcg ggatatttgc gacaaagatg
     6421 tattctaatt ttatctttgt gatatcatcc ccacctgttg ttgtgaaatt taaataaaga
     6481 tctgctggtt cgctgctgtt cccacaggtg aaccatacgt cccaggtatt gatctgtctg
     6541 ttgaatgatg agaaactata gttccaatac tggatatcgt ttaccgatcg cagtttggtt
     6601 ttaaatgatc ttgcggtcat ataatgataa ctgggtgaca cttcgaaatt cgattcagct
     6661 gattgtgcac tggtatcaaa ccagagttga ccatggtacg agttttgtgc tttcccaccg
     6721 acgatgtctt ctacgccagc gaaatcctct gtaagaatgg tcggtgcctc gaatgaccag
     6781 atgtttacaa ttgttggatc atcatcacca gttaacccat ctccgtcctg gtcaatacca
     6841 tcgtctactg taggatcatc cgtgagttcg tttttatcat taatgccatc ctcgttctca
     6901 tcccaatgta caatcccttg gtttgagatt ttcgtgttat ttactaatcc agcattgatg
     6961 gtgaccatgt aacttaatgc gacactgctt tgcgcaggaa tactaccgtc ccagagaatt
     7021 tttttcgttc catcgtcgta ggaaatggta cctgatccgg cagttgcaga tccaggaaca
     7081 tactgggtgt ttgcaggaat gacatcttcg aattcaggac cagggttgtt gttttgattc
     7141 gcggtaccga tattactaat ggtgatcgaa tactcaagga tatcttcaca ttcgacaaga
     7201 tttccattga gatctatcac tgtttttcgt gccatgattt gagttcttgc ataaccagag
     7261 aacctgagat tatcgaagaa cgccatactg tcaaattgat tgtctccttc atcttttaga
     7321 tcgataatga gagtgatgat ttcaccaggg gatactgggt gttcacgccc gataagagcg
     7381 gatgctcctg catctgcacc attggaattt ggtgttgatt gcagccagtc aacccctgag
     7441 ggggcattat cctgagcaaa caaattatag ccggtcccca ggagagctgt atcacgggcg
     7501 tttagaacga aatctcctcc attgacgtta atgatgtatg aggacacgcc ctttgaaggt
     7561 gagttgacag tgatggtaag ctggtcatta tattgtgtac caacgtaatc agggtattca
     7621 accgtgaaga attgaatatc ataatagaga taatgcatgt agggggggac ttccaactgc
     7681 atttggaggt tcgcttcgtc tcgtggggtt ccatattgtc ctccttggaa ccagtttcca
     7741 cgttcgctgc cggggcataa cccgttcgag gtagcataca cgagatctgc tcgacccgtg
     7801 ctgatgacag caaacgatga tccatctgtc ggaataaggt ttccccgcga ggagagaacc
     7861 atggattgtc gatgccctgc ggtatctcga tcccaatact gggaactgat taacgttgag
     7921 ggatcagcta gcatggctat cgcaagatca tgtccatcat acgatccagc ttgtactgag
     7981 tttggggtaa tcgctacgaa tgcaagaatc acctgtaaaa agattatccc tactgtagga
     8041 atttttttgt tcatttccta tccgcctcca ctatagttta tgataaatat tatgtttgat
     8101 tctgcgacga catcatcata catcccatca tttggaatca ccagacattt ccaggattca
     8161 tcaagatgtg tttcttctga gacaatcaca ctacaagcag tagtcccatc ttttgtgcac
     8221 aggtaattct gatagagttg ttctgttgtc agtactcggg gatagatctt cacttcatca
     8281 ataatacctg tgaaattcat ttcaccagcg agtctgatcc cagtgatctc caggtcgtcg
     8341 atcgccatgt atttgtttgc cgctgatgta cgccatttga actgcaactg aaggatattt
     8401 atcgatggag gcagtgaaaa tattacctgt gcccaggacc tctgcccacc aggcatcaat
     8461 ggatttgtta tctcaaaaat aggtttccat tggttcccat tgcgataata tagtccgatg
     8521 aaatcattat tatccgtgtt tttgtagcta taccataagg aaatctctat gtttgagtac
     8581 cctgagacgt cgatccctgt tctgttaaac gcagcgtatt ctgggtcaaa atctccggac
     8641 ccgccaatag agtacaatcc gttatgtggg gttaatgccg tagaggggag cagaccaaaa
     8701 tcacattcta ttcttgcgta gcttgttgca ttgatataga tttgatcgat atagacgaga
     8761 tcatcatcgt agcttgcatc acattggaag cgaagtcgca tgttggttgg gaatcggttg
     8821 ttgctctcat tgatgtagat gatggcatgg taccagacac cttttgtata gatacttgga
     8881 taattcgtat caagaattga aacccaagcc gtcccattat aatatagaag ccaccagtct
     8941 tccccgcttg cccagccgtt cccccgccac atccaccaaa aatctacttt tatggatttg
     9001 tacgctgggg tgtcgatatc aatcgggttc agggtgatcg ctgaagaagt gccgctgtta
     9061 tctcgtatac atgcggaggc ggctccctcg tgtttgtagg cactggatcg gtaactatca
     9121 gctgagcttc cattgcttcc tggaacataa ttcccccatc ctgtttcaaa cgtgtcatat
     9181 cggaggttca tccaagtctg ctgttgtccc aaggtcgcat tgttttcgat ccattcgttc
     9241 ttttcatctt gtgcttcgaa accggttgag aaaatggttt gtctgccatt aataccggtt
     9301 cctttcccaa ccgcaccgac tggggtttcg ccagagccaa ggagtttccc tgcagcatgg
     9361 gtatgctgat acgtatcaag ccgcccatca acatagatag cagaatcacc taatgaagca
     9421 tcatagctag ctgcgatgag atgccaagtg ttatcattta ctggtaccgc tccggtaaga
     9481 tcgattgcac catcacttga gtttgtactc catttcacga atccatctgt cactgcaagc
     9541 tcccagtatt tattccggtt gtatgacaga atggtccctg agttttgact tgtctttacc
     9601 catgcttcga cagtgacgtt gtcaatgtaa ttatcttcaa aacaatatgg aatagaaatg
     9661 aaatcatcac cattaaactg gtatgcccca ccaagttttc cttgatcagt ccaggtggat
     9721 ccgttaatgg ttccgttata gttattccct gagtaatctt tggtttggaa gggattctgg
     9781 ctgtcaaacg gtaggtgaac acgtgtgaga gaagaatacg gaccggttga tgcaaccatc
     9841 caatgataaa tgaaggtaac tggatcaatg ttcggctcaa tggtataatc ataacagatg
     9901 agatcttcat cgatggttct cgttcgcaat gtcgtgacca acataggatc caatacagga
     9961 gacgtcccag gaccaggagg attatctcgg ggggtgatga taccatcgaa aatcattttt
    10021 gtgcttccat ctgaaagcat ggtgaagacg ctgactttca cctgtttttc ttggctaaaa
    10081 agagtatcat tgatcgggag gtaataggat tgccccatca cccagggatc attatcataa
    10141 cgataggtac gtggtccatc tgactgctcc acgcgaatgg tgtaggtagt aagttcctca
    10201 ccaccaacat gctgaaggac aactcgccca tcatcagtga catacccagc aagcttcaca
    10261 tggggatcag gtgacgggag cggtaccggc aaaaactgat aataaataac accaaaggcc
    10321 gctattgcga tcagaacaag cataatcgca ccaatgactt ctgatgctgc ataggaatta
    10381 ttagagaacg acacgttctt catctctcca aaccacaggt acatctctgt tatcgctcta
    10441 tatttaatga tatcgtgcta ttatgctggt ttgccaattt agaagaggat tttaaaggtg
    10501 agaaatgaga tgaggaccag gatgaatgct tggcgtatcc cagcggagag gcgcccgtcc
    10561 atcatgtacc ccccaaggag cccaccgcca aggctttgga caaagacaaa cgtaaaaaac
    10621 atagttttta cctccatcgg gtcgatattt gcg
//
LOCUS       DATMXJ010000216         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_51125, whole genome shotgun sequence.
ACCESSION   DATMXJ010000216 DATMXJ010000000
VERSION     DATMXJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51125"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VN377_05835"
     CDS             complement(<1..450)
                     /locus_tag="VN377_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWR27937.1"
                     /translation="MSGLFGVVSNNNCISSLYYGTDYHTHLGTEYGGIAFIEKNGIPV
                     KKIHDIKNSQFKSKFYEGSDKIKSHLGIGVISDKDPQPLTFENKFGAFAICSAGLVAN
                     KDALATELIEKGVSFSEIDHGEINTTELVAHLINQGNSILYGIERMYD"
     gene            649..864
                     /locus_tag="VN377_05840"
     CDS             649..864
                     /locus_tag="VN377_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27938.1"
                     /translation="MVNTLSHANSTFFHSKNKAASWVFLIVLSNLFFLQKHRKRSSID
                     TLEDLMTMQNLKGTFDHSIPTTGEGEE"
     gene            864..1691
                     /locus_tag="VN377_05845"
     CDS             864..1691
                     /locus_tag="VN377_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27939.1"
                     /translation="MNRQQLTYLRVVALIMAGLVASLTIGNLFTIATKGISVTLPSED
                     EIQWSIDPLQKEILFRTSFSVKNQGVYDIRDIDISAQLVKKDRTPLFSYEKQDMVVLR
                     GTNTTFDVLIPIDLDTISFFDWFSLIYKNTTLQLLLDIDALYMFGLVEFTANEAIDIP
                     WSQPPLNLSDNRTLQAGIQGLCTLFNITQNKSITTISDIFSLFSLPEFYYSSGNGFVF
                     NLTITSYSETLKNISCQIIAPLLIIKGAFEFTVSFLVGFEGNTPIFRIQEVGIEYVA"
     gene            1681..2151
                     /locus_tag="VN377_05850"
     CDS             1681..2151
                     /locus_tag="VN377_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27940.1"
                     /translation="MSHKKHYVSPKALLAFCLYFLIPLVSLFLILHEYPELPSDRVYM
                     RIYWVIPTALIVVLLAQLGSYYKKGETKRFVLNIGFTIMTMIWMFGLLGGGLVMTTFW
                     NEYEFSIHMDKYILLIISVAALNILYYTFEWRVYRKDKSSYLSHGKKKTGTAAE"
     gene            2246..>3535
                     /locus_tag="VN377_05855"
     CDS             2246..>3535
                     /locus_tag="VN377_05855"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HWR27941.1"
                     /translation="MTQDTLAALKDRLSLKDYQKIMNLHNPSINRFIASSLQLCNPER
                     IFVGTDSLEDLQYTRGAALRNHEEKKLAMDGHTVHFDGYHDQARDKAHTKFLLPSGIS
                     LGPELNTMDRDEGLNELHQIMANIMVGHELFIKFYCLGPIHSPFSIPCIQLTDSAYVA
                     HSEDLLYRQGYQEFLRQGNNARFFKFVHSQGALEEAGLGLLVSKNREKRRVYIDLSDE
                     VIYSMNTQYGGNSLGLKKLAMRLAIHRCAQEGWLVEHMLLMGVTGPRGRISYFTGAFP
                     SMCGKTSTAMMKGETIIGDDIAYLRVIDGKVRAVNVEAGIFGIIEGINDANDPLQWDA
                     LTHPAELIFSNILVDNENDVYWNGKPGVRPKNGVNFSGDWSDGKVDDNGKIIPPSHKN
                     ARFTFRLDILPNVDVHLHDPLGVEVHGIIYGGRDSDTS"
BASE COUNT          959 a          799 c          782 g          995 t
ORIGIN      
        1 gtcgtacatc cgttcaatcc cataaaggat gctgtttccc tggttaatta aatgagcaac
       61 aagttcggtt gtgttgatct cgccatgatc gatttcactg aaagaaacgc ctttttcaat
      121 aagctcagtg gcaagggcgt ccttgttggc gacaagaccc gcgctgcata tcgcaaaagc
      181 cccaaattta ttctcgaacg tcagcggttg aggatcctta tctgagatca cccctatccc
      241 tagatggctt ttaattttgt cagaaccttc gtagaacttc gacttgaact gtgaattttt
      301 tatgtcatgg atcttcttga caggaatccc gtttttttcg ataaacgcaa taccaccata
      361 ctcggtcccc aaatgagtat ggtaatcggt tccataatac agacttgaaa tacagttgtt
      421 gttgctgacg acaccaaata aaccactcat agtctccccc gttttgaaat ggaagaacca
      481 atgaattata tataaggatt tttaatgttc ctttccaaat ctgatttttc aaaaccatca
      541 tgattttttt ttatcgatgt cgttttatcg ttttgtaaag attcctcgac atatcttaat
      601 agaagcattg acaaatagta tcatagaatt atcggggagg tagtgtactt ggtaaacaca
      661 ttgagtcacg cgaattctac ttttttccac agcaagaaca aagctgcctc ctgggtattt
      721 ctcatcgtac tatcaaattt attcttcctg caaaaacaca ggaaaagaag cagtatcgac
      781 acccttgagg atctcatgac aatgcagaat ctgaaaggga cctttgacca cagtattcca
      841 accaccgggg agggcgagga gtaatgaacc ggcaacaact cacctatttg agagtcgttg
      901 cccttatcat ggctggtctg gttgcgtcac ttaccatcgg taatcttttt accattgcga
      961 caaaaggcat ctcagtgaca cttccatcag aggacgaaat ccaatggtcc atagaccctt
     1021 tacaaaagga gatcctgttc cgaacctctt tttctgtaaa gaaccaaggc gtttacgata
     1081 tccgagacat cgacatctca gcgcagttag taaaaaagga tcggacaccg ctgttctcct
     1141 atgaaaagca agatatggtc gtgctacgag gcacgaacac cacctttgat gtactgatac
     1201 cgattgatct tgacactatc tcattttttg attggttcag cctcatttac aaaaacacga
     1261 cattgcagtt gctgctcgat atcgacgcgc tgtacatgtt cgggctggtg gagttcaccg
     1321 ccaatgaagc aatcgatatc ccctggtctc agcctcccct gaatctctct gataatagga
     1381 cgctccaggc agggatacag ggtctttgta cactcttcaa catcacacag aacaaatcaa
     1441 tcaccacaat atcagatatt ttttctttgt tttccctccc tgagttttat tattcatcgg
     1501 ggaatggttt cgtttttaac ctgacgatca catcatattc tgagacactg aaaaacatca
     1561 gctgtcagat cattgctccg ctccttatta tcaagggggc ttttgaattc acagtttcat
     1621 ttcttgtcgg ttttgaagga aataccccaa tatttcgcat ccaggaggtc gggatagagt
     1681 atgtcgcata agaaacatta cgtctcacca aaggccttgc tggctttctg tttgtacttc
     1741 ctcattcctc tcgtttccct ttttctgatc ctccatgaat acccggagct gccctcagac
     1801 cgggtgtata tgcgcatcta ctgggtgatc ccgacagcac tcatcgttgt tcttctcgcc
     1861 cagcttggtt cttattataa aaaaggggag acgaaacgat tcgtccttaa catcggtttt
     1921 accatcatga cgatgatctg gatgttcggg ttgctcggtg gtggtctggt gatgacaacg
     1981 ttttggaacg agtatgaatt ctccattcat atggacaaat acatcctgct gatcatcagt
     2041 gtcgcagcac tcaatatatt gtattacacc ttcgaatgga gggtgtaccg aaaagacaaa
     2101 tcatcgtatc tttcccatgg gaaaaaaaag actggtaccg ctgctgaata aacaaagcgt
     2161 ttttcttctt ttcctcctgt tttaaatatc gagagacgat atcaatacga ggtgtttttg
     2221 gaagagaaaa aatgggggaa tgtgtgtgac acaagacacg cttgctgctc tgaaagaccg
     2281 tttatccctg aaggattatc agaaaattat gaaccttcac aacccgtcga taaataggtt
     2341 cattgccagt tctctacagt tgtgtaaccc agaacgtatc tttgtcggca ccgattcttt
     2401 ggaggatctg cagtacacta gaggagcagc attacgcaat catgaagaaa aaaaacttgc
     2461 catggacggt catacggttc atttcgatgg atatcatgac caggcgagag acaaggctca
     2521 taccaaattt cttttaccct cggggataag cctggggcct gagcttaaca ccatggaccg
     2581 ggacgaaggt ctgaatgaat tacatcagat catggcaaac atcatggttg gccatgagct
     2641 cttcatcaaa ttctattgtc tcggacccat ccattcacca ttctcgatcc cttgcatcca
     2701 gttgaccgac tctgcgtatg tcgctcacag tgaggatctt ctgtacagac aagggtatca
     2761 agagttcctc agacagggga acaacgcacg ttttttcaaa ttcgtgcatt ctcaaggagc
     2821 gctcgaagag gctggtcttg gtttgctggt gagtaagaat cgggagaaaa gacgggtgta
     2881 catcgatctt tcagatgagg tcatctatag catgaatacc cagtatggtg ggaacagcct
     2941 tggtctgaag aagctggcga tgcgtctggc gatacatcgt tgcgcccagg aaggttggct
     3001 tgtggagcat atgctcctca tgggcgtgac tggacccagg ggacgaatca gttattttac
     3061 aggggcgttc ccatccatgt gtgggaaaac ctcgacagcg atgatgaagg gagaaaccat
     3121 catcggtgat gacatagcgt atctgcgtgt cattgatgga aaagttcggg cggtgaacgt
     3181 cgaggcaggg atttttggca tcatcgaagg gattaatgat gcaaacgatc cgttgcaatg
     3241 ggatgcattg acccatccag cggagctgat cttctccaac atcctagtcg ataatgagaa
     3301 tgatgtgtac tggaatggaa aaccaggggt tcgaccgaaa aatggtgtga atttttccgg
     3361 ggactggtct gacgggaagg tcgatgacaa cggaaaaata atacccccgt ctcataagaa
     3421 cgccaggttc acgtttcgtt tagatatcct ccccaacgtt gatgtccacc tccatgatcc
     3481 gcttggtgtt gaagtccatg ggatcatcta cggaggacgt gactctgata catca
//
LOCUS       DATMXJ010000217         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_35725, whole genome shotgun sequence.
ACCESSION   DATMXJ010000217 DATMXJ010000000
VERSION     DATMXJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35725"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..2697)
                     /locus_tag="VN377_05860"
     CDS             complement(358..2697)
                     /locus_tag="VN377_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27942.1"
                     /translation="MRNDMWTKGLVVGIIMLFAGTSVVPNITGTIRHGNNESYQQGMN
                     EIKTMYTSAASLYVENVVTPSDPSFRPDDVCTKKFVADKYSQSPNDSSVFTIDNTSLS
                     LPLNNDVLRAGDIIEIKGTANGSTFQYYLIEWGIGEYPTEWFSTGMLLVNDGLIGIVD
                     GTLATWNTSFVNDDDFFTIRLTVNFTSYSSEAFTRNIYLDPSLKEGWPQRINYEYNSQ
                     GGYYYWAGYLETVVDDINNDGHQEIIVYKGGDPPKLDVFCDNGSLLWSSPVGDTDVSG
                     GNLHIPLVGDINNDGFDEIVVFRFLLIGDYSELYVFDHNGAILDGWPIVIPKEYHPTL
                     CIADVNSDGYNEIIFKGNDALDRKLVIIDHTGTILAQWSLSVKQWGSSIESSPATGDF
                     DDDPELEIVCADPSENAGYNSTSGEWNNEGVIHVYNIDGSEVAGWPQYTDGVIFTSPA
                     TGDIDNNGDYEIVVGLQYGGSAPDYRYGGLYAFDKNGNVLPGWPYEKGWNFASAPSLA
                     DFDEDSDLEIATSRLGFYTYVIHHDGTAATGWPQQTTWNDYYSTIIGDINNDNIPDVL
                     TTAGDGFYPSTYQHGGVYAWNYNGTPLPGFPKATEVDAQAPANIADIDGDGTVEIIAS
                     SDWDHDFETSRDKYRGSLYVWEVDAAYNQSTMEWPTFHHDNQRTGMYPSTPSLLDMEI
                     GNITGGLFKVKATIKNIGAVAANDIDWSITVTGGFILLGRETNGSIAALNPGDEQAIA
                     SKPIFGFGKTIVKIIATAPGNTASKEQNATILLFFILMK"
     gene            complement(2979..4055)
                     /locus_tag="VN377_05865"
     CDS             complement(2979..4055)
                     /locus_tag="VN377_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR27943.1"
                     /translation="MKRKCLAVAIILMFVGTCIIPGIAQNTEKKLPTSRGNWFYVGGS
                     GLGNYTRIQDAIDNASDGDTVYVFDDSAPYHEIININESITLFGENQQTTIIDGFDLP
                     DSIITINALYVTLRNLTLRNAEREGVMIHSSYASISQMTIQDSSDYSWIGAITVYFPK
                     QQTRGSIDKILIQNNTIVRTWTGIELSYCNQAMITGNVLLDNINGIEVIGSFHNEIST
                     NFISGCEVGIVDYMSGKNIYRGNTLLNNTCGMEYICSTRDIIEKNNFLNNTQQAFFVK
                     ALYLSLVVIMNMLYSHNLYFLINYRVLRPTIWRQNYWNESRILPYVIVGEAVKDPYNP
                     NGVTKRFNFDMNPAKEPYDIPGMS"
BASE COUNT         1205 a          939 c          768 g         1313 t
ORIGIN      
        1 ttataatcta aataatcagt aatgaaactc ctaatctgat aaacccaagt atcgcttaat
       61 ccttcgattt cccttaattt aataaattca tctaaatatt tcttttctac aggagaagcc
      121 atcgcttcgg gagccataga cttgcgttca aatcgcaaca ggcccatgat tctctctcgg
      181 agaaaactca gtcaacagac ccgtttttgg cattctgttg attttttcca tgtcccgctt
      241 tcccgccatt tctacgtttt ttcaacatgg tttcagaacc aagaacaaag aaaaaaaaga
      301 gggtgatgac cccttcaggt catcatgata ggtggttgaa agcctaaatc atcggtttta
      361 tttcatgagg atgaagaaca gcagtatcgt cgcgttttgt tcttttgatg cggtgttccc
      421 tggagctgtc gcaatgattt ttactattgt ttttccaaaa ccaaaaatag gctttgaggc
      481 aatggcctgt tcatcaccgg gattaagagc tgcaatggac ccgtttgttt ctcttcctaa
      541 gaggataaac ccaccggtta ccgtaatact ccaatcgata tcgtttgccg ccaccgcacc
      601 gatgttctta atggtcgctt tcactttgaa taatccaccg gtgatgttcc ctatctccat
      661 atcaagcaaa gacggcgtgg aagggtacat accagtacgt tggttatcat gatggaaggt
      721 cggccattcc atcgttgact ggttataggc ggcatcaacc tcccagacgt agaggcttcc
      781 tcgatattta tcccgagatg tttcaaaatc atgatcccaa tccgagcttg cgattatctc
      841 aacagtgcca tctccatcaa tatcagctat atttgctggc gcttgggcat cgacctctgt
      901 tgcttttggg aatccaggta gtggtgtccc attataattc caggcgtaca cgcctccatg
      961 ctgatacgtg cttgggtaaa atccatcacc agcagtggtt aaaacatcag ggatgttgtc
     1021 attattgata tctccgataa tcgttgaata ataatcattc cacgtggttt gttgtggcca
     1081 tccggtagct gctgttccat cgtgatggat gacataggtg tagaacccta atctacttgt
     1141 ggcgatctcc aggtcggaat cctcatcgaa atctgcaagg gacggagcgg aagcaaaatt
     1201 ccatcctttt tcataaggcc agcctggtaa tacattccca tttttatcaa acgcatacag
     1261 tcccccatag cgataatcag gagcagaacc accatactgc agaccaacga caatttcata
     1321 atctccgtta ttatcaatat ctccagttgc aggggatgta aagataaccc catcggtgta
     1381 ctgcggccag ccagctactt cagatccgtc aatgttatac acatggatga caccttcatt
     1441 gttccattct cctgatgtcg aattatagcc agcgttttca ctagggtctg cacagacaat
     1501 ttctaactca ggatcgtcat caaaatcccc tgttgctgga cttgattcaa tggaactacc
     1561 ccactgtttc acagataatg accattgtgc tagtatcgta cctgtatgat cgattattac
     1621 aagtttccga tcaagagcgt catttccttt aaagataatt tcgttgtatc catcactatt
     1681 tacatctgcg atacacaacg ttggatgata ttcctttggg atgacaatag gccaaccgtc
     1741 gagtatcgca ccattgtggt caaaaacata taactctgaa taatccccaa tgagcaggaa
     1801 tcggaacaca acgatttcat cgaatccatc attattgata tcaccaacaa gaggtatatg
     1861 caggtttcca ccggaaacat ctgtatctcc aacgggggac gaccataaaa gtgagccatt
     1921 gtcacagaac acatcaagct tcggtgggtc accaccttta tacacaatta tctcttgatg
     1981 tccatcgtta ttgatatcat cgacaaccgt ttctaaataa ccggcccagt aataatagcc
     2041 accctgggaa ttatactcat aattaattct ctgtggccat ccttctttca ggcttggatc
     2101 gaggtatata tttcttgtga atgcttcact tgaataactt gtgaaattaa cggtaagccg
     2161 tatcgtaaag aaatcgtcat cattcacaaa gctcgtattc caggttgcta aggtaccatc
     2221 tactatccct atcagcccat cattcactaa gagcatcccg gttgaaaacc attcagttgg
     2281 atactcacca attccccatt ctatgaggta atactgaaac gttgacccgt tcgcagtccc
     2341 ctttatttca atgatgtccc ctgctctcaa aacatcattg ttcaatggca gtgataacga
     2401 cgtattgtca atagtgaata ctgagctatc attaggtgac tggctgtatt tgtcagcaac
     2461 gaattttttc gtacacacat catcaggtcg gaacgatgga tccgatggtg ttacaacatt
     2521 ttcaacatag agtgaggcgg cagaagtata cattgttttt atttcattca tcccctgttg
     2581 atatgattcg ttatttccat gtctgatagt accagtgata ttcggtacaa cacttgttcc
     2641 agcaaacagc ataatgatac ctacgaccaa tccttttgtc cacatatcat ttctcatgtg
     2701 agaaccctcc ctgttgataa caggttactt gttaatatac tcttgattca tttaaacctt
     2761 cgttttttat ccgataatat taatatatcg gaataaacaa aaaagagagg cgttaagacc
     2821 ctgaagggtc aataacgata ttccctccct ttcagctacg gtctttgtgc gtctacggtc
     2881 ctcatttcca agtcctttga ataaattact atagcggtcg tgcgaaatca aatcaactat
     2941 ctctcaggca tcattcgaca atttcagatg gtgtcttttc aactcatccc tgggatgtca
     3001 tagggttctt tagcagggtt catatcgaaa ttaaaccgct tggtaacccc atttggatta
     3061 taagggtcct ttacagcttc cccgacaatg acatagggta atatccgtga ttcattccaa
     3121 tagttttgcc gccaaatggt agggcgtaat acccgatagt ttattaaaaa atataaattg
     3181 tgagaataca acatattcat tattacaact aacgataaat aaagtgcttt cacaaaaaac
     3241 gcttgttggg tgttatttaa gaagttattt ttttcaatga tatctcttgt cgaacagata
     3301 tattccatac cgcaagtatt gtttaacaag gtatttcccc gatagatatt ttttccgctc
     3361 atataatcga cgatgccaac ctcgcatcca gagatgaagt tagtggaaat ctcattgtga
     3421 aatgagccaa taacctcgat gccattaata ttatccaaga gcacattccc agtaatcatg
     3481 gcttgattac aatatgacag ttcaatccct gtccaggttc taacaatagt attattttga
     3541 atgagaattt tatcaataga gcctctggtt tgctgtttag gaaaatacac tgttattgca
     3601 ccaatccaac tataatcgct ggaatcttga attgtcatct gagagattga tgcataacta
     3661 ctgtgaatca taactccctc tcgttctgcg ttccgaagag taagattgcg caatgtcaca
     3721 tacaatgcgt taatcgtgat tatactatct ggtaaatcaa aaccatcaat tatggttgtt
     3781 tgttgatttt caccaaatag tgtgatagat tcattgatat tgattatctc atgatacggt
     3841 gctgagtcgt cgaagacata aactgtatct ccatcactcg cattgtcaat cgcatcctga
     3901 atcctcgtat aattccctag tccactccca ccaacataga accagttccc tctcgatgtt
     3961 ggtagttttt tctcggtgtt ctgtgcaatc cctggaatga tgcaagttcc aacgaacatg
     4021 agaatgattg caaccgccaa acatttcctt ttcatttgat tttccttgcc tttgatttta
     4081 tattaaggat atcttgctca ctcattatgt ggatttgaaa ttatgtattt ttattcctaa
     4141 tgaaattgca gtaagcaagg aggagctgcc agtgaaggtt gacttatgag taataaaaaa
     4201 accataataa tcatgattat aatac
//
LOCUS       DATMXJ010000218         9852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_62129, whole genome shotgun sequence.
ACCESSION   DATMXJ010000218 DATMXJ010000000
VERSION     DATMXJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62129"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..7273)
                     /locus_tag="VN377_05870"
     CDS             complement(<1..7273)
                     /locus_tag="VN377_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27944.1"
                     /translation="MYDVHETLKRATIFFLIGTLLASSYNGTANIVRDAIPVNDNPTL
                     PTLSRNEIIQTQSNENPQQLNLSIQQNTISVNNHTSIALPSSYTGWYNKPTSYAQMIS
                     WYQELEIAYPNYLQVFKANELYGLGTIPNQNYDMYYVRLTNESLGFQKPEVLFMTNIH
                     GNEYIGPTSAFWFTDWLLRHAFHPNYTCPERDWLRWLLDNREIYIIPSQNPDGFDDVR
                     RENFYGLDLNRDFDHSRPNPWAAVNSQILRRFIDNHTIRIAVDMHTGYRGIPYSWSNM
                     NYRTTLGAISPISGRNYPGYCPPDFYFLDAALLRVGNYVGDYGGNLYSSNIGPWPYTL
                     GYDADGTSCDWWNGGDVITNPAEDPYVNDEIYGNYPGCGIMGHVIEYGPHNPANSEYG
                     NDTINRYGAEVRRTMLHQIDLAQPYLRWLNGTTTNNTIAELETPLTFNWQVNGSLVVD
                     NTFIQWGRNPDPLNNPEYNTTDYDDHAGDYYGGTGWDGANNGQTNGVTYSENIMITTP
                     GDYYFVAKAKVDQRYKTVLHPEEYGNSSYLRLIKERTNDSFYESRLGTDGLEVIDGQT
                     WWYSPVIHVHVSDWWNTSWQYRKKISIDHTKVAANLTNYPLLINLASNIDLIGKIQND
                     AGDIVFTDKNRNKLNHEIELYNYSTGQLIVWVNVTRLSHITNTTLYMYYGNQGCQNQE
                     NPSGVWSSSYQGVWHLNSNFLDSTIYNNDVTNYGSTPTAGWIGDSQYFAGDDYITAPT
                     HCLGTNHSWTASCWFRSNSSSPMLFHYFFSTGHYDANNAVTIWQNINSPGNIGELRAQ
                     IVDSDGHRANMFNYGGTNFKDNVWHLLHVTWNASSHDLAVYGDGVLQRQQINTTVDTG
                     GGTTNPLYIGTRIDLEAQRFHVGELDELRISNPVKNTSWIATEYNNQRTPDSFYIVGS
                     EETIITQHPPILLNPLPLDNAIDTQINPLLSIQVIDPEGDTMNVTFRTNASGIWVTIG
                     SNLSVINGVYSHHPTQMNNYHTTYYWSVNITDPYGSKLWTNITYRFTTSFEPGGWWNT
                     QWFFRKSIVIDHTQISSTLLNFPMLMSISSDPTLAMHAKTNGSDIVFTDYYGHQLNHE
                     IESYESTTGNLEAWVKIPILSSSQDTSLYMYYGNPLCNNQETPLGVWETNYQGVWHLN
                     DDVLDSTSHDNDATNHGSINTSGQIGKGRFFDGNDYLSAPKHYLGAEWSWTASCWFYS
                     NASSMIYHYLLSTGQYDSPSAVSLYHNINHIEYPPIGEMRARINDMDNHFIQLYQYNG
                     VFPKGTWNLVHLTWDANSHTFSVFINGSLENRSTNTAVDIGGGAQTGLFMGARQDFDP
                     QRYLVGKLDEVRIATSLKNASWIAAEYVNQYNPNEFYNLGVEEVGLTPTEPTFWDEHP
                     ATDASDIGLQPILSIRTIDYQGDTFDLYFKTNASGTWQTIGGNLSIGNGTYSCTNTLT
                     INNYLTKYWWSVNATDASSDNWTNLTFRFTTRPKNYVPLIVNVNPAHGDTNVPLNPTL
                     SIQISDRDGDIMNITFKTNTTGIWKTIGSYNNTGNGTYTQTTTNMSTLGKTYYWRINI
                     SDSKGNWTNATHWFKTVTTMLSLKWMIDLGGGAKGSPCVANVTGDSTPEVIAVTSSGV
                     KCIDSQTGSVIWSRSYSGIGEWVQPEVADLNNDEVPEVLVPIQNPPVGLLVLYGNNGS
                     TYWVRRDLGGEWSFGVPITYDIDGDGYPTVFFASTDVSHGMNGTGRVTALSYDGQILH
                     QAFSWRPCAGGLSLADYNFDGIFELYMNDRSEYPGGKGLRSFWASNLTCRWNQTGTGT
                     ISSHVTILVDVNNDGLLECVVAPQSTTGGVMVLSAVDGHIMKGSTEMNIPAHEKFTVY
                     DIDHDGRLESIHAENEHPGMPKDIVVWDLVNWQRDATITIPTTCYWPPIAAEVTGDSI
                     LDIVAGGNGRLMVFNQNYSSVASYTGLQQLQQPVVNDIDGDGYNEIVVLSDSGRLYSF
                     DTPGITPTPTPRSVKHYSEYRRNAAEYVEPPGPRYPMQRDESPTNKSTLVTFNPVLSV
                     HVWDYQWDRFNITFRTNASGTWQDIKTYASIGRYPSHTITGNSWGRYTANTTNMTQPN
                     KTYYWSIYAKDIKGNTHEKIYHFTVGDVAPIITNPLPVDGVNGVSLSLSTLQFTLTDC
                     QGNRMNYSVYTTPNIGTGSRTLVTNGTYTISLNGLEYNTFYTWFVHITDGTHWTNKTY
                     HFTTRLSPSIWWNNNWTFRKEIIIDNTQVAGPLTNFPVLINLNSDYDLATHVQDDGDD
                     LVFTDHARNILYHEIELFNGTTGQLVAWVNVTSLSSTSPTLLYLYYGNPAVSSQQNPT
                     GVWKSNYLTVHHLEETSGALSDSTSYHHNGTPYGGLQQNITGIIDGADRFDGVNDYID
                     LGATPDFNKQDLTISAWVKSSVPTTDMRIVVGGASYTYKWHLIMDDGYFMMTNNTLAT
                     TN"
     gene            complement(7380..>9852)
                     /locus_tag="VN377_05875"
     CDS             complement(7380..>9852)
                     /locus_tag="VN377_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HWR27945.1"
                     /translation="NIPMSIAINSRMAGGAAMWDRMQDELNYSDYSWEPSIHTRTHPC
                     GTYTNGYVWEILGCRNDILENLTKIPYGQYVFNYILPCGYQDSTIESTSAGEFLFLRD
                     WTGSDHPSSTIYAPWNSVYNFYGIGGLETKAYDPVFEARSPSGRYYASDVTTLNNAFT
                     TVYNNGGIFYAMFHPDRYSNSVIFDTRPGVDGVSGSSFMQHLSFVANRTNVWYVANGW
                     LYSYHYVAENAVVSGGQMHLSDIPMTIVNNNANTYAYHTLTNLVNGTTYQYYVTGIDD
                     PGHLVRIPSAPTFRDFTVRQPSAWWNMNWHYRKEITIDYTKVASNLTNFSILIALASD
                     ADLASKTQPDGDDIVFTNSTGSKLSHEIELFNSTTGQLIVWVNVTSLSSTQDTILYLY
                     YGNSACANQENSAGTWASNYRMVQHLSETTGIHDDSTTYGNDGTYHGSNQNAVGIIDG
                     ADQFIGDLVGPGDYIDCGNATSLNINSAITVSSWIKPDDQVNWNHLFTKGCNTPNRVY
                     QFSIEANETIDFILNGDTTNAKATTAVSVPINQWSYVTGTYDGSSIKVYINGIERANK
                     AYTNPINTNAVHLFIAGRVNGTGNTGPSTYTFNGLMDEVKLSDNARSAGWILTEYNNQ
                     YNPSGFYQMGSEEEGAQLEYIYTIPVQTHWNLISLPFNTSLIKSEINISYNGTTYTWN
                     QAISNNIIIGFLYGWNRTGQSYLLQNTLEPGCGYWMWAYHPCDLVISLDGEYQDGLQI
                     TTLQQRWNIIGLPYNVSLAKQNITVQYNGTDFTWQQATGGSTPIILGYIYGWNRATQI
                     YQLSDQLNAGYGYWMYAYHQCVLKT"
BASE COUNT         2613 a         2020 c         2213 g         3006 t
ORIGIN      
        1 ggttcgtggt cgcaagcgtg ttattcgtca tcatgaaata gccatcatcc atgatcaaat
       61 gccatttgta ggtgtaggaa gctccaccga cgacgatcct catatccgtg gtgggtacgg
      121 aggatttcac ccaggcggag atggtcagat cctgtttgtt gaaatcaggg gtcgcgccaa
      181 ggtcaatgta atcgttcacc ccatcaaacc gatcagcacc atctataata ccagtaatat
      241 tctgttgtaa tccaccatat ggtgttccat tgtgatgata tgaagtgcta tccgagagag
      301 ccccagaggt ttcctcgaga tgatgtacag tgagataatt tgatttccat accccagtcg
      361 gattctgctg ggaagaaaca gcaggatttc cataatatag atatagaaga gtcggagaag
      421 ttgaagaaag acttgtcaca tttacccatg caacaagttg acctgttgtc ccattaaaga
      481 gttcaatctc atgatataat atatttctcg catggtctgt aaagaccaga tcatcaccat
      541 catcctgtac atgtgtagca agatcataat cagagtttag atttataaga acagggaagt
      601 tggtcagtgg tcctgcaacc tgcgtattgt caatgatgat ttctttacgg aaggtccagt
      661 tattgttcca ccagatgctt ggacttagtc tagtggtaaa atgataggtt ttattggtcc
      721 aatgtgtccc atccgtaata tgaacaaacc acgtgtaaaa agtgttatat tcaagaccat
      781 taagggaaat cgtgtaagta ccgttggtga caagcgttct actccctgtt ccgatgttag
      841 gagtcgtata aacagagtaa ttcatccgat ttccttggca atctgttaat gtgaactgaa
      901 gtgttgaaag agacagggac actccgttga ctccatcgac gggaagtgga tttgtgatga
      961 ttggagcgac atcaccgacc gtgaaatggt atattttctc atgggtgttt cctttgatat
     1021 cctttgcgta gatgctccag taataagttt tgttcggttg agtcatatta gtggtgttgg
     1081 ctgtgtatct tccccaggag ttaccagtga tcgtatgact cggataccgg ccaatagagg
     1141 cataggtttt aatatcttgc caggttccgg acgcattggt ccggaaggta atgttgaacc
     1201 gatcccattg ataatcccag acatgtacag aaagtacagg attgaaggtg acaagcgtgg
     1261 acttattagt aggggattca tcgcgctgca ttggatatct tggaccgggg ggttcaacat
     1321 attctgccgc atttcttcgg tattcgctat agtgtttgac tgatcgaggt gtaggtgttg
     1381 gtgttatccc tggggtatca aaagaatata accgccctga atcacttaag acaacaattt
     1441 cattgtatcc gtcaccatcg atatcgttca ctacaggctg ttgaagttgc tgaagacctg
     1501 tgtagctagc aacagaagaa taattctgat taaagaccat taaccgtcca tttccaccag
     1561 cgacaatatc taagatgcta tcaccggtca cctccgccgc gataggtggc caataacagg
     1621 ttgtcggaat agtaattgtc gcatctcgtt gccagttcac taaatcccaa acaacaatgt
     1681 ctttgggcat accaggatgt tcattttcag cgtgaatgga ctcaaggcgt ccatcatgat
     1741 caatatcata aacagtgaat ttttcgtgag cagggatgtt catttctgta ctacctttca
     1801 tgatatgtcc atccactgca gaaagaacca tgaccccacc ggttgtcgat tgaggagcaa
     1861 cgacgcattc gaggagccca tcattattga catcgacaag gattgtgaca tgactactaa
     1921 tcgtccctgt acctgtttga ttccagcgac aggtcagatt gcttgcccag aacgatcgga
     1981 gtcctttacc accagggtac tccgaacgat cattcatata gagttcaaag attccatcga
     2041 agttataatc agctagagag agaccacccg cacaagggcg ccatgagaag gcttgatgga
     2101 gaatctgacc atcataggat aaagccgtta ctctaccagt tccattcatt ccatgcgaaa
     2161 catcggtgga tgcgaaaaac acggttgggt atccatctcc gtcaatatcg taggtgatgg
     2221 ggacgccaaa actccattca ccacccaaat ctcttcgaac ccaataagta ctgccgttat
     2281 tgccgtataa aacaaggagg ccgacagggg gattctgtat tggaactaat acctcgggga
     2341 cctcatcatt atttaaatca gctacctcgg gttggaccca ttcccctatt ccactatagc
     2401 tgcgtgacca gatcacactc ccggtttgag agtcgataca tttgactcct gaggaggtca
     2461 ctgcgattac ttcaggtgtc gaatcaccgg tcacatttgc aacgcagggg ctgcctttcg
     2521 cacctcctcc taaatcaatc atccatttaa gactaagcat tgtagtgact gttttaaacc
     2581 aatgggttgc atttgtccaa ttacccttcg aatcagagat attaattctc caatagtagg
     2641 ttttaccaag ggtactcata ttagtggtgg tttgcgtgta ggttccgtta ccagtgttgt
     2701 tgtaactacc gatagtcttc cagattccgg tggtatttgt tttaaatgtg atattcatga
     2761 tgtccccatc tcgatcgctt atttgaatcg agagtgttgg attgagtggt acgttcgtat
     2821 ctccatgagc aggattcaca ttgacgatga gaggaacata gtttttcggt cttgtggtga
     2881 atcggaaagt gagatttgtc cagttatctg aactggcatc agtggcattt acactccacc
     2941 agtatttggt tagataattg tttatagtta gagtattggt acaactatag gtaccatttc
     3001 ctatgctaag attacctccg atagtttgcc atgtgccaga agcgttggtt ttgaaataga
     3061 gatcaaatgt atccccttga taatcaatag tgcgtatgga taatataggt tgtaatccaa
     3121 tatccgaagc atcggtagcg gggtgttcat cccaaaatgt tggttcggtt ggagtaagac
     3181 caacctcttc aactccaagg ttgtagaatt catttggatt atattgattt acatattctg
     3241 ccgcaatcca tgatgcattt ttcaaggaag ttgctattct tacttcatct agcttcccca
     3301 ctaaataacg ttgcggatcg aaatcctgtc gagcgcccat gaataaacct gtttgcgcgc
     3361 cacctccaat atctacagct gtattggtag atctgttttc taatgaacca ttgataaaca
     3421 cagaaaacgt atgagaattt gcatcccagg ttaaatgaac caaattccat gtccccttag
     3481 gaaagacacc attatattga tagagttgaa taaaatggtt atccatatca tttatgcgtg
     3541 ctcgcatctc gccgataggg ggatattcta tatgattaat attatggtag aggctaacag
     3601 cacttggact atcatattgt cccgttgaga ggaggtaatg ataaatcatt gaagaagcat
     3661 tggaatagaa ccagcaactt gctgtccaac tccattccgc tccaagatag tgctttggag
     3721 cgctgaggta atcgtttcca tcaaaaaatc gtccttttcc aatttgtcca ctagtattaa
     3781 tggatccgtg gtttgttgca tcgttgtcat gtgaggtgct atcaagaaca tcgtcgttaa
     3841 gatgccagac tccttgataa tttgtctccc agacccctaa gggcgtttct tggttgttac
     3901 agagtggatt cccgtaatac atatacaatg aggtatcttg tgaagaacta agtatcggga
     3961 tcttcaccca tgcctcaagg ttgccagtag tactttcata ggattcgatt tcgtggttga
     4021 gctgatgtcc ataatagtca gtgaagacaa tatcgctacc attcgttttt gcgtgcattg
     4081 cgagagtagg atcagaggag atgctcatga gcatggggaa atttaaaaga gttgaggaga
     4141 tttgtgtgtg gtcaataacg atgctttttc ggaaaaacca ttgtgtattc caccatcccc
     4201 caggttcaaa ggaagtggtg aagcgatagg tgatattcgt ccatagttta ctcccataag
     4261 gatcagttat gttcacactc caataataag tagtatggta attgttcatt tgggttgggt
     4321 ggtgggaata cacaccattg attacgctaa gattacttcc gattgttacc cagattccag
     4381 aggcattggt tcggaacgtg acattcattg tgtctccttc tggatcgatt acctgaatag
     4441 aaagcaatgg atttatctgg gtatcaatcg cattatcaag cggaagggga tttaataaaa
     4501 ttggaggatg ttgagtaatg attgtttctt cagatcctac aatataaaag gaatctggag
     4561 tacgttggtt gttatattct gtcgctatcc aactcgtgtt ttttactggg tttgagatac
     4621 ggagttcatc gagttctcca acgtggaagc gttgagcctc tagatcaatt cgtgttccta
     4681 tatagagcgg attcgttgtt ccgccaccag tatcaactgt tgtgtttatt tgttgtcgtt
     4741 gtaagacacc atcaccatat acagcaagat cgtgtgatga cgcgttccat gtgacatgga
     4801 ggagatgcca aacattatct ttaaagtttg tacccccgta gttgaacatg tttgcacgat
     4861 ggccatctga atcgacaatt tgtgctcgca gttccccgat gttacctggg ctgttgatgt
     4921 tctgccagat ggttaccgca ttattggcat cataatggcc agtggaaaag aaataatgaa
     4981 agagcatcgg tgaggacgaa ttggagcgga accagcaact tgctgtccag ctatgattcg
     5041 tacctaaaca atgtgtcgga gcagtgatgt aatcatcccc agcaaaatac tgactgtccc
     5101 ctatccagcc tgctgtaggc gtcgatccat agtttgttac gtcattatta tagatagtgc
     5161 tgtccagaaa attgctatta agatgccaga ctccttgata acttgaactc cagactccac
     5221 tgggattttc ctggttttgg catccttgat ttccatagta catatagagt gttgtatttg
     5281 taatatgtga aagtcgtgtc acatttaccc atacaatcaa ttggccggtg ctgtaattat
     5341 agagttcgat ttcatgattc agtttatttc tatttttatc cgtgaacaca atgtcaccag
     5401 catcattttg aatcttacca atcagatcta tgttactagc aaggttaatc agtagaggat
     5461 aattggtcag attcgcagct actttggtat gatcgataga gatcttcttt ctgtattgcc
     5521 aactagtatt ccaccaatca gatacgtgaa catgaattac agggctgtac caccaggtct
     5581 gaccatcaat aacttctaaa ccatctgtgc ctagacgtga ttcatagaat gagtcattgg
     5641 tacgttcttt gattaatcgg aggtaagaag agttcccgta ctcttctgga tgaagaacgg
     5701 ttttatatct ctgatcaact tttgcttttg caacaaagta atagtcacct ggtgtagtga
     5761 tcatgatatt ctcagaatat gtgactccat tggtttggcc attgtttgca ccatcccatc
     5821 cggttcctcc gtagtagtct cccgcatggt catcgtaatc tgtggtgtta tactctggat
     5881 tgttgagcgg atctggattt ctcccccatt gaataaaagt attatcaact acaaggcttc
     5941 cattcacctg ccagttgaaa gtgagaggag tttcgagttc tgcaattgta ttgtttgtgg
     6001 tagtaccatt caaccatcga agatagggtt gcgctaaatc aatctgatgt aacatagttc
     6061 ggcgtacttc cgccccatat cggttaatgg tatcatttcc atactcactg tttgctggat
     6121 tgtgtggacc atactcaata acatgaccca tgattccaca gccaggatag ttaccatata
     6181 tttcgtcatt gacatatgga tcttcagcag gattcgtaat aacgtctccc ccattccacc
     6241 aatcgcaact tgtaccgtct gcatcataac caagggtgta aggccaaggt ccgatgttac
     6301 tcgaatagag gtttcctcca tagtcaccaa catagttacc gacacggagt agcgctgcat
     6361 ctaaaaaata aaaatctggg ggacagtacc ctgggtaatt ccgcccggag attggactta
     6421 tcgcccctaa tgtggttcga tagttcatgt tgctccaact gtaggggatt ccccgatatc
     6481 cagtgtgcat atctacagca atacgtatgg tatggttgtc gatgaatcgc cggaggatct
     6541 gtgagttcac ggcagcccat ggattgggtc gactatgatc aaagtctcgg ttgagatcta
     6601 ggccatagaa attttccctc cgaacatcat caaagccatc tgggttttga cttgggatga
     6661 tgtagatttc tcggttgtcg agaagccaac gaagccagtc tcgttctggg catgtgtaat
     6721 ttggatggaa cgcatgtcgg agcagccaat cggtgaacca gaatgcacta gtaggtccga
     6781 tgtactcgtt cccatgtatg tttgtcatga agagcacttc tggtttttgg aaccctaatg
     6841 actcgtttgt gagtctcaca tagtacatat cgtaattttg atttggtatt gtccctagtc
     6901 cgtagagttc atttgctttg aatacttgaa gatagttcgg gtatgcaatc tctaattcct
     6961 gataccagct aatcatctga gcataacttg ttggtttatt gtaccaccct gtataagaag
     7021 aaggaagagc aatactagta tggttgttaa ccgagatagt attctgttga attgaaagat
     7081 ttaactgttg tgggttttca tttgattgag tctggattat ttcatttcta gatagtgttg
     7141 gtagtgttgg gttatcgtta acaggaatag cgtctcgtac tatgttggct gtcccattat
     7201 atgaacttgc gagcaatgtt ccaatgagga aaaaaatcgt cgctctcttg agcgtttcgt
     7261 gtacatcata cataatatca aatatcccct tgtgtaaagt acaatctagt ttattctgaa
     7321 tctttcattc tctaatccgg ttatttgcag tatttatgcg taatgagtaa tcggggagat
     7381 cacgttttta gcacgcattg gtgatacgcg tacatccagt atccataccc agcattcagt
     7441 tgatctgata attgatagat ttgagttgct cggttccatc cgtagatata cccaaggatg
     7501 atgggtgtag atccacctgt tgcctgttgc catgtaaaat cagtgccgtt atactggacg
     7561 gtaatgtttt gttttgcaag ggaaacattg tatggaagac ctatgatatt ccatctctgt
     7621 tgaagcgtgg tgatttgcaa accatcttgg tattctccat caagtgagat gacaaggtca
     7681 cagggatgat acgcccacat ccaatacccg caaccaggtt ccaaggtatt ctgtaataga
     7741 tagctttgac ctgttctgtt ccaaccatag aggaagccga taataatgtt attactgatt
     7801 gcttgattcc aagtataggt cgttccatta taggatatat taatctcgga tttaatcaga
     7861 gatgtgttga agggcaaaga gatgaggttc caatgtgttt gcactggtat cgtatagata
     7921 tattccagtt gtgctccttc ctcttcactg cccatctggt agaatccact ggggttgtat
     7981 tggttgttgt actctgtgag aatccatcct gcactacggg cattgtcaga gagttttact
     8041 tcatccatca aaccattgaa agtatatgtg gaaggtcctg tatttccagt gccgttcacc
     8101 cgaccagcta tgaagaggtg gacagcattt gtattaatcg ggtttgtgta ggctttattt
     8161 gctcgttcaa ttccgttaat atacactttg atagagctgc cgtcgtatgt cccagttaca
     8221 tatgaccatt gatttatcgg tacgcttaca gctgttgttg ctttagcatt tgtagtatcc
     8281 ccattgagaa taaagtctat ggtttcattt gcttcgatac tgaattgata cactcggttt
     8341 ggagtattac aaccttttgt aaagaggtgg ttccagttca cttgatcatc tggtttaatc
     8401 catgaagaaa ccgtgattgc actattgatg ttgagacttg tagcatttcc gcagtcaata
     8461 tagtcaccag gacctacaag atccccgatg aactgatctg caccgtcaat gattccgacg
     8521 gcgttctgat tgctcccgtg gtaagtccca tcgttgccat atgtggttga gtcatcatgt
     8581 atacctgttg tttcactgag atgctgaacc atgcggtaat ttgaagccca ggttcccgcg
     8641 ctgttctctt ggtttgcaca tgcgctattt ccataataga gatataagat agtgtcctga
     8701 gtacttgata gagaggtaac gttcacccag acaatgagtt ggccagttgt actgttaaat
     8761 aactcgatct catgcgataa tttactacca gtggaattgg taaagacgat atcatcacca
     8821 tctggttgcg ttttactggc aagatcagca tcagaagcaa gagcaatcaa tatggagaag
     8881 ttggtgagat tacttgcgac tttcgtgtaa tcaatggtga tttcttttcg ataatgccag
     8941 ttcatgttcc accatgcact tggttgacga accgtgaagt ctctgaatgt aggtgctgag
     9001 gggattcgaa cgagatgtcc agggtcatca attccagtga cataatactg gtatgttgtc
     9061 ccattaacga ggtttgttaa tgtatggtat gcataggtgt ttgcattatt gttcacgatt
     9121 gtcattggga tatcgcttag atgcatttgt ccaccagaaa cgactgcgtt ttcagcgaca
     9181 taatgatatg aatacaacca tccatttgcc acgtaccaca cattggtccg gttcgcgacg
     9241 aacgagaggt gttgcatgaa tgaggagccg cttacaccat caacgcctgg tcgtgtgtcg
     9301 aagatgacgc tgttactgta ccggtcagga tggaacatcg catagaagat tccaccgttg
     9361 ttgtaaacag tagtgaacgc gttgttcagc gtagtgacat ctgatgcata atatctccct
     9421 gagggggatc gggcttcgaa gactggatcg tacgcctttg tttctaaccc accaatgccg
     9481 tagaaattgt aaacactatt ccagggtgcg tagatcgtgc ttgagggatg atcgctccct
     9541 gtccaatctc tgagaaataa aaactcgcca gcagaggtac tctcgatggt gctatcttga
     9601 tacccacagg ggagaatgta attgaataca tactggccat aggggatctt tgtaagattt
     9661 tctaagatgt catttcggca tccaaggatt tcccatacat atccattcgt gtaggtacca
     9721 cacggatgtg tccgtgtatg gatggacggt tcccagctgt aatcagagta gttcaactca
     9781 tcttgcatcc gatcccacat cgctgctcca ccagccatcc ggctgttgat ggcgatagac
     9841 atcgggatgt tg
//
LOCUS       DATMXJ010000219         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_41155, whole genome shotgun sequence.
ACCESSION   DATMXJ010000219 DATMXJ010000000
VERSION     DATMXJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41155"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="radA"
                     /locus_tag="VN377_05880"
     CDS             complement(<1..955)
                     /gene="radA"
                     /locus_tag="VN377_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HWR27946.1"
                     /translation="MGKDKENESPLHDLPGVGPAIADKLIEAGYGDMMSIAVSSPKEL
                     AEVAEIGEPTAAKIIQAARKQADIGSFETGTALLERRAKIGSITTGSKAFDELMGGKG
                     FDSQAITEMFGEFGSGKTQIVHQMCVNVQFPVEKGGLDGHVFYIDTENTFRPDRIIQM
                     AEAYELDSDEVLNKIHVARAYNSSHQMLLVDKVIELSKEFPARLLIVDSLTSQFRAEF
                     VGRGALADRQQKLNKHMHDLLRWSDLFNGVVVVTNQVAAKPDAFFGDPTRPIGGHIVG
                     HTATFRLYLRKSKGGKRIARLIDSPHLPEGEAVFSVSEKGIR"
     gene            1113..2609
                     /locus_tag="VN377_05885"
     CDS             1113..2609
                     /locus_tag="VN377_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27947.1"
                     /translation="MKLLNRTKMLVNAGVVLAITLAFLMPVTASLTKTPMVTITHQQP
                     KTFFGNAEWIEQASGFWETSQGIHYMCAVDENIVWAVGYDGSTSQLPVQEFTRTTNGG
                     DLWVADLIDGAPADGDSAMIFALDDMTAWVPIHTGTPQGIWKTSDGGQTWAQQTTADF
                     TGGFPNCVHFWNENDGWCMGDPVGGYYEIYTTTDGGDNWVRVPQADIPAPSSSAEYGV
                     VGYYDVVGDTVWFGTQDASFGGRVFKSIDKGYHWTVSSVIFAPGSYVDIRFKDALNGL
                     AMDKNFADAVLAETSDGGLTWTGITYSGKCHGADFDYVPGTSNMYVSTGVQTGVPENN
                     GATYSFDGGHSWTSWAEMEGIQLFGTAWVEGKIGWAGNFNVDDTTGGVWKHLPSETPK
                     PVLTLSIVGGKGFTVTVKNVGEAAATNVTCDVTIAGGLFIKPKDFSITQGTLGSGGNF
                     TLVGAPKGIGLGFLKPKPTITINVNCTEGIGATMSVNAKIFLSKVTLG"
BASE COUNT          719 a          578 c          600 g          753 t
ORIGIN      
        1 ccctgattcc tttttcactg acagaaaaca ctgcttctcc ctcaggaaga tgcggggaat
       61 cgatcaatcg tgcgatccgt ttgccccctt tgcttttccg cagatataac cggaacgtcg
      121 cagtatgccc cacaatgtga cctccaatcg gcctggtcgg gtcaccaaag aacgcatctg
      181 gtttagcggc gacttgattg gttactacaa cgaccccgtt aaacaggtcg ctccaccgta
      241 ggagatcatg catatgcttg ttcaatttct gctgtcgatc cgcaagtgcg cctcttccga
      301 caaactccgc acggaactgg ctggtgagtg aatcgacaat taacaaccgt gcaggaaact
      361 cttttgatag ctctatgacc ttatcgacga gaagcatctg atggctcgag ttgtacgccc
      421 ttgcgacatg gattttattt aacacctcgt cagaatcaag ctcataggcc tctgccatct
      481 ggatgatacg atccggtcga aacgtgtttt ctgtgtctat gtagaacaca tgaccatcca
      541 atcctccttt ctctactgga aattggacgt tcacgcacat ctgatgtact atctgggttt
      601 ttcctgagcc aaactcacca aacatttctg tgatcgcttg gctatcaaat ccttttccgc
      661 ccatcagttc atcaaaggct ttggaccctg tggtgattga gcctattttt gctcggcgtt
      721 ccagcagcgc agtgcctgtt tcaaaactcc cgatgtccgc ttgctttcga gctgcttgaa
      781 tgatttttgc agcggtgggt tcaccgattt ctgcgacctc tgctaattcc tttggtgatg
      841 agacagcaat gctcatcata tctccatacc ctgcttcaat taatttatcc gctattgcag
      901 gaccgactcc aggtaaatca tgcagaggtg actcattttc tttatctttt cccataagaa
      961 ctcccttatg taagaagaaa gagaaagatg tttataaaca tgtgggaaag gagactgaaa
     1021 gtatcctcta actcagagaa actcacctcg atatttttta cgaaacattt aaataaaaag
     1081 acgactatga tgataaatat ggggaaaaac atatgaaact tttaaacaga acaaaaatgt
     1141 tagtaaacgc tggtgtcgtt ctcgctatta ctttggcatt tctcatgcca gtaacagcat
     1201 cgcttacgaa aacacctatg gtgaccataa ctcatcaaca accaaaaacc ttttttggga
     1261 atgctgaatg gattgaacaa gcatctggtt tttgggaaac tagtcaaggt atccattaca
     1321 tgtgcgccgt agatgagaat attgtttggg ctgtaggtta tgatggaagt acttctcagt
     1381 tacctgttca agaatttacc cgaacaacaa atggcggtga cttatgggta gcggatctca
     1441 ttgatggagc accagctgat ggtgacagtg ctatgatttt tgccttggat gatatgactg
     1501 catgggttcc aatacacaca ggtacccctc aaggtatatg gaagacatca gatggaggtc
     1561 aaacctgggc tcaacaaacc accgctgatt tcaccggtgg attcccgaac tgtgtgcatt
     1621 tctggaatga aaatgatggc tggtgcatgg gagatcccgt tggtggatat tatgaaatat
     1681 atacaaccac cgatggagga gataactggg tcagagtccc tcaagctgac attcctgcac
     1741 cttcatcgtc agcagaatac ggtgttgttg gatattatga tgttgttggt gatacggttt
     1801 ggtttgggac tcaagatgct tcatttgggg ggcgtgtctt taaatcaatt gataaaggat
     1861 atcactggac agtttcaagt gttatttttg ctcctggttc ctatgtcgat atccgtttca
     1921 aagatgcatt aaacggccta gctatggata agaattttgc agatgcagtt cttgctgaaa
     1981 catcagatgg tggtttaact tggaccggta tcacatactc tggtaaatgc catggagcag
     2041 attttgatta tgttccagga acatctaata tgtacgtaag tactggagtg cagaccggtg
     2101 ttccggaaaa taacggtgca acatacagct ttgatggcgg tcattcatgg acatcatggg
     2161 ctgaaatgga aggtattcaa ctctttggta cagcatgggt tgagggtaaa atcggatggg
     2221 ccggtaactt taatgtcgat gatacaaccg gtggagtttg gaagcattta ccctctgaga
     2281 caccaaaacc agtgttgacg cttagcatcg tgggcggaaa aggtttcacc gtgactgtga
     2341 aaaacgttgg tgaagcagcg gcaacaaatg tcacctgtga cgtcaccatc gccggtgggt
     2401 tattcataaa accaaaagac ttctcaataa cccaagggac tcttggtagc ggcggtaact
     2461 tcaccttagt tggtgcacca aaaggtatcg gtcttggttt tctcaaacca aaaccaacga
     2521 taacgatcaa cgtgaactgc accgaaggta ttggtgcaac catgtccgtc aacgcaaaga
     2581 ttttcctctc gaaggtaacc ttagggtaaa acgagaaaaa atctctcttt tttttttttt
     2641 atttgacaaa
//
LOCUS       DATMXJ010000220         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_24580, whole genome shotgun sequence.
ACCESSION   DATMXJ010000220 DATMXJ010000000
VERSION     DATMXJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24580"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VN377_05890"
     CDS             <1..431
                     /locus_tag="VN377_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HWR27948.1"
                     /translation="LKLVTATSLLDRASIVIAGMEIDVVNQIVNDPSVIEDGLRIVKR
                     EKPVKTGSIDLYGFDKNHVPVVIEVKRSLATISSVHQLRMYVTDIKKDRKEPKVRGIL
                     CAPRVPDMVKKLLEEHGLEWREVERKVILPDNRQRTLKEF"
     gene            complement(466..849)
                     /gene="pth2"
                     /locus_tag="VN377_05895"
     CDS             complement(466..849)
                     /gene="pth2"
                     /locus_tag="VN377_05895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HWR27949.1"
                     /translation="MSPRKKNQSDFEYKMVIVTRSDLPLSAGKLAAQVSHVAVDCTLL
                     TKKNKSEWFSKWQREGAKKVVVKVDCLDDFFSLKEKAEKLGICTSIISDAGHTEIPEG
                     TQTVLGIGPAPSNLIDQVTGELPLL"
     gene            complement(815..1078)
                     /locus_tag="VN377_05900"
     CDS             complement(815..1078)
                     /locus_tag="VN377_05900"
                     /inference="COORDINATES: protein motif:HMM:NF039840.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5749 family beta-barrel protein"
                     /protein_id="HWR27950.1"
                     /translation="MICKFVVDGSGKKIGESVSIDHDVLIIKAGTLFLGVPLKHVESG
                     GKTLMVRGIFDFTKAYELGEKWRKESYREMKKHEPPEEKSKRF"
     gene            1253..2446
                     /locus_tag="VN377_05905"
     CDS             1253..2446
                     /locus_tag="VN377_05905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWR27951.1"
                     /translation="MEKYDVAVVGGGPIGCYVAEQLASKGRQVAVFEEHHTIGEPVHC
                     AGLVTRRVMDITKCPQDEIVQNTIYGAIIHSPAGALLTIGGEKIHALVIDRQRFDDHI
                     AKNAQKAGATILRDHTVIAAKKQRSSLTLDIQHDGQVTLVHSPLLIGADGAHSRIRDS
                     FGFPQPIEKLHGISAELSDTSLNPKYVHIFVGNTIAPGFFAWVIPTNTHGTTARIGLC
                     IGKHSTHPLQHYYTTLLQQPLLQGTTVLKRFGGTIPLGPLKKTTADHIMLVGDAAAQV
                     KPTSGGGIYPGLLCATTCSTIAEEALKKQCFDEEVLRRYHTTWTKEIGRELFLGMRFR
                     RIFTRLTDSHFNKYIEKLNTQKTIDTINTYGDIDYPSHLALPLLTAVPSLLSLAPTLL
                     KRNNK"
     gene            complement(2449..4665)
                     /locus_tag="VN377_05910"
     CDS             complement(2449..4665)
                     /locus_tag="VN377_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27952.1"
                     /translation="MRRCVVYQILVVFLLLGCFYNVSVSVAESASDSNVDATLTCSFV
                     SGAQLTVQAQMIVNSIDVFETVYTKQAIEEMAATNQYVMGAIMLRLHEVVKSQIEAAF
                     KDADVDTMNTNPTYEKPFFIDVFRVNLTPAFFQYAGSLDLIDFINGVLDMGATVAYSF
                     DLSAEQGWNTTFLYTLPSTMTLVYANTADTNPDTNTVRWVVRNWDGADAGIDATLSMQ
                     SKNPTTIASETDEIALEFILDARTATDINFIDSVIVKKVNIRPYNVLPAFVTGLGSIP
                     ADGVRLFIENGLMSWTTLFEKTIQPIEQQTTHIIENSSFQQNLSLSFQWDAETTINCS
                     TPYNITHMDESPAIRANFQDPEINLMICQMPARALFGLINAGANVSISSEDVNFGSGL
                     NGIRYPYVIMLRLPTNITLDGETDYLWNDTSSISGAFTSGLQPTPPYRAEHVETFVDI
                     ELVKMDLNIPSVFTGRTEMTASVKMREDDNLYVIRRADELPFSPKVELSYLNADAFRL
                     CAEEQVFGEEQILTFLSEKTALFQQRLSNILHGLKAKGAVDKPSFTSSLVWDGDISAM
                     DDLAPVVVSNYANEVYMIGFNVSLWPASISIVPQQFAFQGIQNQTVTYRIIFPRGIEL
                     NASESTGKPLIIGTTNDGREYVELTFDELAAVNSTVLTCVLQASPVYILGLFLPCILV
                     FLLLVVLIVIIYLIRKKRGGLRRGKRKLFEPEDNEPSDYIEQEYYVPPPPSTKKKK"
BASE COUNT         1368 a         1248 c          947 g         1178 t
ORIGIN      
        1 aactcaagct ggtgaccgcg acatcgcttt tggatcgcgc aagcatcgtc atcgccggga
       61 tggaaatcga cgtggtcaac caaatcgtaa atgatccatc cgtcatcgaa gacggcctcc
      121 gaatcgtaaa acgtgaaaaa ccagtgaaaa ccgggtccat tgatttgtat ggatttgata
      181 aaaaccacgt gcctgtggtc atcgaggtga aacggagtct tgcgacgatt agctctgtcc
      241 atcagcttcg catgtacgtg accgacatta aaaaagaccg aaaggaaccg aaggtacgtg
      301 gaattctttg tgcgccacga gtccctgaca tggtgaaaaa gctccttgag gagcatggtt
      361 tggaatggcg agaggtcgaa cgaaaagtta tattaccaga caatagacag aggacattga
      421 aagagtttta acattaaaaa ataggttatt atttagctga atatatcata ataacggcaa
      481 ctccccggtc acctgatcaa tgagattgct cggcgctgga ccaatcccaa gaaccgtctg
      541 cgttccctcc gggatctccg tatgccctgc atcggaaatg atcgatgtgc aaataccaag
      601 cttctctgct ttctctttca acgagaaaaa atcatcaaga caatcgacct tcaccacgac
      661 tttttttgct ccctcccgct gccatttcga aaaccactcc gatttgttct tcttcgtcag
      721 aagcgtacag tccaccgcca catgagacac ctgcgcagca agcttcccag cagaaagagg
      781 aagatcagaa cgagtcacaa tcaccatttt atattcaaaa tcgctttgat ttttcttccg
      841 ggggctcatg ttttttcatc tcacgatacg attcttttcg ccatttctca cccagctcat
      901 acgcttttgt aaaatcaaag atgcctctta ccatcaatgt tttcccaccg gactcgacat
      961 gcttcaaagg tacccctaaa aacaatgttc ctgctttgat aatcaacaca tcatgatcaa
     1021 tcgacacgct ctcccctatc tttttccctg aaccatcgac gacgaactta cagatcatag
     1081 aaggatcaac attctcgata gatttctgcg tttcggtatc ctcttttttc ttattaaaaa
     1141 agaatatgtt accaatatat gctgaagtca ctaacacaat attaattatc aaaaccatgg
     1201 gtccctgtaa taccgttttt attataaatc tttgttgata ttctgaacat caatggaaaa
     1261 gtacgatgtc gcggttgttg gcggaggacc tatcggttgt tatgtcgcag aacagctagc
     1321 atcaaaagga agacaggtcg cggtgttcga ggagcatcac actatcggag aaccagtaca
     1381 ctgcgctggc ctcgtcaccc gacgcgtaat ggatattaca aaatgtcctc aagacgaaat
     1441 agtccaaaac acgatttatg gcgcaatcat tcacagccca gcaggggctc tcctcaccat
     1501 cggtggtgaa aaaatccacg ccctcgtcat cgaccgccag agattcgatg accacatcgc
     1561 taaaaacgcg cagaaagcag gggcgacgat tctgcgagat catacagtca tcgcagcaaa
     1621 aaaacaacgc tcatctctca ctcttgacat ccaacatgat gggcaggtga cgcttgttca
     1681 cagcccgctc ctcatcggtg ctgacggcgc ccattcacgc atacgagaca gcttcggttt
     1741 tcctcaaccc atcgaaaaat tacacggcat cagtgcagaa ctctcagata ccagcttgaa
     1801 tccgaaatat gtccacatct tcgtcggaaa caccatcgcc ccaggttttt tcgcctgggt
     1861 catcccaacc aacacacacg gcaccaccgc acgcatcgga ctgtgcatcg gaaaacacag
     1921 tacacatccc ctgcaacact actataccac cctgctgcaa cagccactgc tacaaggaac
     1981 caccgtccta aaacgattcg gaggcacaat ccccttaggc ccactgaaaa aaacgacggc
     2041 tgatcatatc atgcttgtcg gagacgcagc cgcacaagta aaaccaacct cgggaggtgg
     2101 catctatcca ggattactct gcgcaaccac ctgcagcacc atcgctgaag aagccttaaa
     2161 aaaacagtgt ttcgatgaag aagtcttacg acgctatcat actacttgga caaaggagat
     2221 cggccgagaa ctctttctag ggatgagatt cagacgcatt ttcacccgtc tcaccgacag
     2281 tcacttcaac aagtacatcg aaaaactcaa cacacaaaaa accattgaca ccatcaacac
     2341 ctacggggat atcgattacc cttcacatct cgcgctccca cttctcaccg cagtaccctc
     2401 tctgctgtct cttgccccaa cgctcttgaa acgcaacaac aagtaagatt atttcttctt
     2461 cttcgttgac ggcggcggag gcacataata ctcttgctcg atgtaatcag acggctcgtt
     2521 atcctctggt tcaaacaact tccgtttccc tctccgcagt cctcctctct tcttccgtat
     2581 caaatagatg atgacgataa ggaccactaa caacagaaag acaaggatgc aaggtaaaaa
     2641 caagccaaga atgtagactg gtgacgcttg caacacacag gtaaggaccg ttgagttcac
     2701 cgcagccagc tcatcaaacg tcagctcaac atactctcgt ccgtcattgg tcgttccaat
     2761 gatcagaggt ttgcctgtac tttcgcttgc attgagttca atgcctcgtg ggaagataat
     2821 ccgatacgtc accgtctgat tttggatccc ttggaatgcg aactgttgtg gtacaatgga
     2881 aatagaagct ggccataacg acacgttaaa cccaatcatg tacacttcat tggcataatt
     2941 agagacgaca acaggcgcca gatcatccat ggcagagatg tccccatccc acacaaggga
     3001 actggtaaac gagggtttgt ccacggcacc tttcgccttc aatccatgga gaatgttcga
     3061 taacctctgc tgaaacaacg cggttttctc tgagagaaac gtgagtatct gctcttcacc
     3121 aaacacctgt tcctcggcgc acagacgaaa cgcatccgcg ttcaaatatg aaagctcaac
     3181 cttcggggaa aatggtaact catccgcacg tctaatcacg tacagattgt catcctctct
     3241 catcttcaca gatgctgtca tttcagttct ccccgtaaag acactcggga tgttcaaatc
     3301 catcttcacc aactcaatat caacgaacgt ctcgacatgt tctgccctgt acggtggagt
     3361 cggctgcagc cctgaagtga acgccccaga aatcgaagag gtgtcgttcc agagataatc
     3421 cgtctctcca tccagagtga tgttcgttgg gagacgcaac atgattacat agggatatct
     3481 gatcccattc agccctgaac caaagtttac atcctctgag gagattgaca cgttagctcc
     3541 tgcgtttatc aatccgaata atgcccgggc aggcatctga cagatcatca ggttaatctc
     3601 aggatcctga aaattcgctc gtatcgctgg cgattcgtcc atatgggtga tgttataggg
     3661 cgtggaacaa ttgatggttg tctccgcatc ccattgaaat gacagactca gattttgttg
     3721 gaacgaggag ttctcaatga tatgtgttgt ctgctgctcg ataggttgaa ttgttttttc
     3781 aaaaagggtc gtccagctca ttaatccatt ctctatgaat aaccgtactc catctgccgg
     3841 aatgcttcct aacccggtca caaaagcagg aagcacatta tatggtcgga tgttgacctt
     3901 tttcacgatc accgaatcaa taaaattaat atcagtcgcc gttcgcgcat ctaggataaa
     3961 ttcaagagcg atctcatcag tttctgatgc gattgtcgtt ggattttttg actgcatcga
     4021 taacgttgca tcgattcctg catcagcccc atcccaattc cgaaccaccc agcgcaccgt
     4081 gttcgtatca ggattcgtat ccgctgtgtt cgcgtacacc agtgtcatcg tgctcggcag
     4141 cgtatacaaa aacgtcgtat tccacccttg ttctgcggac agatcaaaag aataggcaac
     4201 tgttgcaccc atatccagaa caccattgat aaaatcgatc agatcaagag aaccagcata
     4261 ctggaaaaag gctggggtca gattgactcg gaacacatcg ataaaaaatg gtttttcata
     4321 cgtaggattc gtattcatcg tgtcaacatc cgcatcctta aatgctgctt cgatctgtga
     4381 ttttactacc tcatggagac gtaacataat ggcgcccatg acatattgat tagttgccgc
     4441 catctcttca atcgcctgtt tcgtatagac ggtttcaaaa acatcgatac tgttgacaat
     4501 catctgcgcc tgcacggtca actgagcacc agaaacaaaa ctacaggtaa gcgtcgcatc
     4561 gacattacta tcggaagcag attccgcaac actcactgaa acattataaa aacatccaag
     4621 taaaagaaaa actaccaaga tttggtacac tacacatcgt cgcatcgcat cctcactttc
     4681 taaggaacta agcaaaaact cttataaaaa ctttcttacg aaatcactaa aaagacatgg
     4741 a
//
LOCUS       DATMXJ010000221         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_20129, whole genome shotgun sequence.
ACCESSION   DATMXJ010000221 DATMXJ010000000
VERSION     DATMXJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20129"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VN377_05915"
     CDS             complement(<1..698)
                     /locus_tag="VN377_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR27953.1"
                     /translation="MMLSSYLSPMNPELQKILTRQHYAIIGNHSGVKLCHWMKQSLLF
                     GRQCYKQTFYGINSHQCLQMTPAITHCTQMCLFCWRLQNFTEKELHDPDDPGFILEHA
                     ITAQRKLLTGFKGDPRCDQKKWKEANEPNMLACSLSGEPTLYPRLGEFFELCRQKGIT
                     TFLVTNGTKPEALEKLSTLPTQLFVSLVAPTEELYKKICAPLVPNGWEKIQQTLELLP
                     SLSTKTVIRHTLVHG"
     gene            complement(762..893)
                     /locus_tag="VN377_05920"
     CDS             complement(762..893)
                     /locus_tag="VN377_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27954.1"
                     /translation="MNQHCSLCGTNLLPADEEGLCHLCKMSIIFNEETFPEVDDCAS"
     gene            1070..2383
                     /locus_tag="VN377_05925"
     CDS             1070..2383
                     /locus_tag="VN377_05925"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWR27955.1"
                     /translation="MKIDPWSSTTYQDYARLRDEFGIQEFSEELWKDLPHPQRLLRRG
                     VVFGHRGFPIIHDAIKNNKPWAILTGLMPSGRMHLGHKMVIDEVLYYQSLGADIFIAV
                     ADIEAYATRGFTLEQTKELAVNEYILNYIALGMDLKKCHLYFQSKHQDVKDLAFLLGK
                     KVNWSQMVATYGFEGSTNMAHIFSPLVQTGDILHVQLEKYGGIRPTLVPVGVDQDPHI
                     RLSRDIAQAFRLYNVMVTKDDKIGVFVKVDTNVKELMDAAENTLKNLQFTDLRRITEY
                     KAIYINDALEKDIPIIDEALAHIEPSFQGYGFLQPSSTYHRFITGLTGDKMSSSKPES
                     AIFLTDTPQEAKKKIMNAKTGGAVTLEEQKRSGGKPDECMIYELFLYHLIEDDGELKS
                     IYESCKKGQLMCGMCKKHAVELMEKFLVDLQEKRKKALGQIQEFL"
     gene            complement(2389..2898)
                     /locus_tag="VN377_05930"
     CDS             complement(2389..2898)
                     /locus_tag="VN377_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27956.1"
                     /translation="MKIKPRNDILKDIIRDGKKHPKGWNATFGKDSTSFSHDCYIFHP
                     SIGIYLLKEYNKNPFEVKGVGSKLARHIDDDIEEQMRKKSEDFGIIQGDIRKILTNIN
                     RGVPPEQILHSAINGDDLGLTIPVRGRASTSKDTFHSLKNTFSHQQKKLETCFEKMVS
                     DDGLYTSYE"
     gene            3008..3409
                     /locus_tag="VN377_05935"
     CDS             3008..3409
                     /locus_tag="VN377_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWR27957.1"
                     /translation="MAKQVITIDADASVFDACIAYKEKKVGCLVVTKGESCVGIVTER
                     DLIERTICQQRSPEKTKVYEIMSKNIKVVSSMDTVEKALETMKQYKIKKLPVVSSEKV
                     VGIITVTDIAKARPDLSERFMESWMKAQWRD"
BASE COUNT          974 a          740 c          833 g          905 t
ORIGIN      
        1 ccatggacca gggtgtgacg tatcactgtt tttgtagaaa gcgacggcag aagttccagg
       61 gtctgctgta tcttttccca gccgtttgga acaagcggtg cacagatctt tttgtacaac
      121 tcttcggtcg gggcgacaag agaaacaaaa agttgtgttg gcagggtaga aagtttttct
      181 aatgcctcag gttttgtccc gttggtgact aggaaggtgg tgatgccttt ctgacggcac
      241 agctcaaaga actccccaag ccttgggtac agcgtaggtt ctccagacag cgagcaggca
      301 agcatgtttg gttcgtttgc ctctttccat tttttctgat cgcagcgagg atcgcctttg
      361 aacccggtga gaagcttcct ttgcgcggtg atggcatgtt ccaggataaa tcccgggtcg
      421 tcagggtcat gtaactcttt ttctgtgaaa ttctggagcc gccagcagaa cagacacatc
      481 tgtgtgcagt gagtgatggc tggcgtcatc tgcagacatt gatgggagtt gataccgtag
      541 aaggtttgtt tgtaacattg tctaccgaac aggaggcttt gcttcatcca atggcagagt
      601 ttcacaccag aatgattccc gatgatcgcg taatgctgtc gagtaaggat tttctgtaac
      661 tcaggattca taggtgatag ataggacgat aacatcatgt tttatatcaa ttatgctaca
      721 aaacacgcgt cctctcctaa aaaaaaaaat aatggttttt tttatgaggc gcagtcgtca
      781 acctctggga aggtttcctc attaaagatg atggacatct tgcagagatg acagagacct
      841 tcctcatcgg caggcagaag atttgttccg cagagcgaac aatgctggtt catggaaaaa
      901 actccaaggt attaccccta gaagccataa tggaaagcgt atgatataaa gtatttgttt
      961 aatagtaaac cttatatcat gattattttc ccctccctag gttttctagg cttctgtgac
     1021 gataacggta agtttttcta aggatatctg atttggaacc tgaacaccta tgaagataga
     1081 cccatggagc tcaacaacct atcaggatta cgcacggtta cgtgatgaat tcgggataca
     1141 ggagttctcc gaagaactct ggaaagacct gccacatccg caacgactcc tccgacgagg
     1201 cgtggtgttc ggccatcgag gattccctat catccatgat gcgattaaaa acaacaaacc
     1261 atgggcgatc ctcactggtc tgatgccatc cggacggatg cacctgggcc ataagatggt
     1321 catcgacgag gtgctgtatt atcagagcct tggtgccgat atcttcatcg cggtcgcaga
     1381 catcgaggcg tacgccacaa gaggattcac ccttgagcag acaaaggagc ttgcggtaaa
     1441 cgagtatatc ctcaattaca tcgcgcttgg gatggacctc aagaagtgcc atctgtattt
     1501 ccaatcaaaa caccaggatg tcaaagacct cgcatttctg ttaggcaaaa aggtgaactg
     1561 gtctcagatg gtcgctacct acggtttcga gggatcaacg aacatggccc atatcttctc
     1621 ccccctggtg caaaccgggg atattctcca cgtgcaatta gagaaatacg gggggatccg
     1681 accgacgctg gtcccggtgg gcgttgacca ggatccacat atcaggctca gtcgtgacat
     1741 cgcccaagca tttcgtctct ataatgtgat ggtcacaaag gacgataaaa tcggtgtgtt
     1801 cgtcaaggtc gatacgaatg tcaaggagct tatggacgct gcggagaaca ccctaaaaaa
     1861 cctccagttc acggacctcc gacggatcac ggagtacaag gctatctata tcaatgacgc
     1921 attagaaaaa gacatcccga ttatcgatga ggcacttgct catatcgagc cttcattcca
     1981 agggtacgga tttttacagc catcatccac gtatcatcgg ttcatcaccg ggttgactgg
     2041 ggataagatg tcctcatcta aacctgagag tgctattttc ctcaccgaca cgcctcaaga
     2101 ggcaaaaaag aagattatga atgcgaaaac cggtggtgct gtaactctgg aggagcagaa
     2161 gagatcaggg ggaaaaccag atgaatgcat gatctatgag ttgtttttat atcatttaat
     2221 tgaggatgat ggtgagctga aaagtatcta tgaatcatgc aagaaaggac agttgatgtg
     2281 cgggatgtgc aagaaacacg cagttgaact tatggaaaaa ttcctcgttg atttacaaga
     2341 gaaacgaaaa aaagctctgg gacagataca agagtttctt tagtagcatt attcatagga
     2401 cgtatagagt ccatcatctg aaaccatttt ttcgaaacag gtctcaagtt tcttttgctg
     2461 atgggaaaac gtgttcttca gagaatgaaa tgtgtctttt gaggtcgacg cccgacctcg
     2521 tacagggatc gtgagtccca ggtcatcccc atttattgca ctatgcagga tttgttctgg
     2581 gggtacccct cggttgatgt tcgtaagaat cttacggata tccccttgga tgatgccgaa
     2641 gtcctcggat tttttcctca tctgctcttc gatgtcatcg tcgatatggc gtgcgagttt
     2701 tgatccaacg cctttgacct cgaaagggtt tttgttgtat tcctttaaca ggtaaatccc
     2761 aatgcttggg tggaagatgt aacagtcatg ggaaaacgac gtagaatcct tcccgaacgt
     2821 cgcgttccaa cctttcggat gtttcttgcc gtctcggatg atatctttca agatgtcgtt
     2881 tctcggtttt attttcacag acatccgtta tactatggca agataagaat ataacggaga
     2941 gacgaaatac tctgtcttaa gtagcaagga aaaaaatggt gtgaacgaca tgctggtaaa
     3001 agagatcatg gcaaaacagg taatcaccat tgatgctgac gcgtccgtct ttgacgcatg
     3061 tatagcctac aaggagaaga aagtaggctg cctggttgtg acaaaaggtg aatcttgtgt
     3121 tggtatcgtc acagaaaggg atctcattga acgaaccatc tgccaacaac ggagccctga
     3181 aaaaaccaag gtatacgaga ttatgtccaa gaacatcaaa gtcgtcagtt caatggatac
     3241 tgtagaaaaa gcacttgaga ccatgaaaca gtataaaatc aagaaactcc cggtggtctc
     3301 ctcagaaaaa gtcgtaggca tcatcacggt gaccgatatc gccaaagcca gacctgacct
     3361 ctcagaacga ttcatggaat cctggatgaa agcacaatgg agagattaaa aagataagaa
     3421 aatcgactgt ttttttattc aagtgtttta aa
//
LOCUS       DATMXJ010000222         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_46420, whole genome shotgun sequence.
ACCESSION   DATMXJ010000222 DATMXJ010000000
VERSION     DATMXJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46420"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VN377_05940"
     CDS             <1..965
                     /locus_tag="VN377_05940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR27958.1"
                     /translation="PTRELCVQVTDVFKDFGKTLGIRTTSVYGGVSIGPQIRDIRTSE
                     IVIGTPGRMLDHLRRKTIDLRNIRILVLDETDKMFEMGFVDDVEDIIRYIPSERQLLM
                     FSATISEEVYRLARRHLKNPLIVKTQSYVDHSKLKQIYYDIYEQNQKFSLLVHLLNSN
                     TDGLSIVFCGTRRESDAVSKNLARQGISASAIHGGMKQQKRSSSLDALKTQKTKVLVA
                     TDVAARGLDIKNVTHIYNYDVPKTHTEYVHRIGRTARAGANGAAITLLTERDHDNFRR
                     VQNNDDFQIERADIPDFRKVPFLRRFEPRHNSFRGSSPRHSFGR"
     gene            1113..2888
                     /locus_tag="VN377_05945"
     CDS             1113..2888
                     /locus_tag="VN377_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HWR27959.1"
                     /translation="MRIAIVLKDRCTSKRCAQECIKFCPRVRAGDETVVMGEDGRPII
                     SEELCVGCGICVNKCPFEAIKIIGLAEELKEDLVHQFGKNGFRLYRLPIPKKGSCVGI
                     LGQNGIGKTTAIKILAGQVIPNLGEYRKKKPSLDPVLEYYAGMELFEHFKALSEQNMT
                     CVLKPQYVDQLPRIVKGKIRDVLKSSDTTDSFDSVVAKLGISQLLDKEIEKGTISGGE
                     LQLVSIAAALIKDVDLYFFDEPTSYLDIYQRLKVARIIQELSKEKKVIVVEHDLAVLD
                     FLCENVHIMYGTEGAYGVVTFPRTVRHAINTYLSGYLKEENIRFGTKIEFFANPPKPQ
                     QGLRILVSYDNLIKEFEDFTLQVEQGIIRQGELVGIVGPNAIGKTTFVKMLAGVIQPT
                     KGAIEYDLKISYKPQYITPDFEGTVHEYFERFAPQLFSSSFLQAEVHDALHLKYLQDR
                     ELMTLSGGELQRVAIAASVVKEADIYLIDEPSAYLDSQQRMIVSRMLRRLIEKSGKSA
                     MIVDHDVYFIDMVSDALIVFDGQPGRQGRAQGPFSLHEGMNRFLKNVDITFRRDEETK
                     RPRVNKPDSYMDRQQKQNGEYYYKF"
BASE COUNT          872 a          680 c          683 g          735 t
ORIGIN      
        1 ctcctacacg agaactgtgc gtgcaggtaa cagacgtctt taaggatttt gggaaaaccc
       61 ttgggatacg gaccacgagc gtgtacggtg gtgtgagcat cggaccacag atacgcgaca
      121 tccgcacatc cgagatcgtc ataggaaccc ctggcaggat gcttgatcat ctccgcagaa
      181 agactattga tttacggaat atcaggattc ttgtccttga tgaaaccgat aagatgttcg
      241 agatggggtt tgtcgacgat gtcgaagata tcatccgcta cattccttca gagcgacagt
      301 tgctgatgtt cagcgccacg atctccgaag aggtgtatcg tctcgcgaga agacatctga
      361 aaaatccttt aatcgtcaaa acccaatcgt atgtcgatca cagcaaatta aaacagatct
      421 attatgatat ttacgagcag aaccagaagt tctcccttct cgttcatttg ctgaacagca
      481 acaccgatgg gttatccatt gtgttctgcg ggactcgcag agaatctgac gcggtctcaa
      541 agaaccttgc gcgtcaaggc atcagcgcct cagcaatcca tggagggatg aaacaacaaa
      601 aacgatcaag ctccctggat gcgttgaaaa cccaaaaaac caaggtatta gtcgcgacag
      661 atgtcgcagc acggggtctt gatatcaaaa acgtcaccca tatctacaat tacgatgtgc
      721 caaaaacaca taccgagtat gtgcatcgga tcggtagaac cgcccgtgcc ggagcaaatg
      781 gcgcagcaat caccctgctt accgaacgag atcatgacaa ctttcgaagg gtacagaaca
      841 atgacgattt ccagattgaa cgagcagata tccctgattt tcgcaaggtg ccgtttttac
      901 gacgatttga gcctcggcat aattcgttcc gaggatcatc cccacgacat tcctttggtc
      961 gatgaagaag tactcctttg gggtcatcaa agaaacatga acaagtgatt tgatacattc
     1021 actttttttt ttaattttta acagcatata ctttttgatg gataggcacc aaaaaacctt
     1081 taagaacctg cctcttcaca gtgttcgcag atatgagaat cgcaatcgtg ttaaaggatc
     1141 gatgtacatc aaagcgatgc gcgcaggaat gtataaaatt ctgccctaga gtccgtgctg
     1201 gtgatgaaac cgttgtgatg ggtgaggacg gcagaccaat catctccgag gagctttgtg
     1261 tcggctgcgg catctgcgtg aataaatgcc ccttcgaagc aatcaaaatc atcggacttg
     1321 ctgaagaact caaagaggac cttgtccacc aattcgggaa aaacgggttt cgactgtatc
     1381 ggctcccgat cccgaaaaaa ggaagctgcg ttggtatcct aggtcagaac gggataggaa
     1441 aaacaaccgc tattaagata cttgcaggac aggtcatccc aaaccttggg gagtatcgta
     1501 aaaagaaacc atccttagac cctgtgcttg aatactatgc aggcatggag ctctttgaac
     1561 atttcaaggc tctatcggag cagaacatga cctgcgtgct caaaccacag tatgtcgatc
     1621 aactccctcg gatcgttaaa ggaaaaatta gggacgtact gaaaagttca gatacgactg
     1681 atagttttga ttcagttgtc gcaaagcttg ggatttctca actccttgat aaggagatcg
     1741 aaaaaggaac gatctctggt ggtgagctcc agcttgtgtc cattgctgct gctctcatca
     1801 aagacgttga tttgtatttt tttgatgagc caacctccta tcttgatatc tatcagcgat
     1861 taaaagtcgc aaggatcatc caggagcttt ccaaggaaaa aaaagtcatc gtggttgagc
     1921 atgatttagc ggtcttggat ttcctgtgtg aaaacgtcca tatcatgtat ggaactgagg
     1981 gtgcatatgg tgttgtgaca ttcccccgga ctgtacgaca tgcgatcaac acctatctct
     2041 ccggatatct aaaggaagaa aacatccgtt tcgggacgaa aatcgagttt ttcgcaaacc
     2101 ccccaaaacc acaacaaggt ctccgtatcc tggtctcgta tgacaatctt ataaaggaat
     2161 ttgaggattt cacccttcaa gttgaacaag ggatcatccg acaaggcgaa cttgtcggta
     2221 tcgtcggccc caacgcgatt ggaaagacaa catttgtcaa aatgctggct ggagtcatcc
     2281 aaccaacgaa aggcgccatc gaatatgacc tgaaaattag ttacaaaccc cagtacatca
     2341 ctcctgattt cgaaggcaca gtccatgaat attttgaaag attcgcacca caactgttct
     2401 cctcatcgtt cttacaagcc gaggttcatg atgcgcttca tttgaaatac ctgcaagacc
     2461 gtgaattgat gaccttgtca ggcggtgagc ttcaacgggt agcgatcgca gcatcggtcg
     2521 tgaaagaggc tgatatctac ctcattgacg aaccatcagc atacctggat agccaacagc
     2581 ggatgattgt ctcacggatg ttacgccgat tgatagagaa atcagggaaa tccgccatga
     2641 tcgtcgacca tgatgtctat ttcatcgaca tggtcagtga cgccttgatc gtctttgatg
     2701 gtcaaccagg gcggcagggt cgtgcgcagg ggccgttcag ccttcatgag gggatgaacc
     2761 gattcttgaa aaatgtcgac atcactttcc gacgagatga ggagaccaaa cgacctcgtg
     2821 tgaacaaacc tgattcctat atggaccgtc aacagaaaca gaacggtgaa tattactata
     2881 agttttaaat tctgctggaa ataacaatgc aattgtgcga taaatatagg ataaataaca
     2941 accaggaaat ctgataaatc cgactttcct
//
LOCUS       DATMXJ010000223         8533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_14899, whole genome shotgun sequence.
ACCESSION   DATMXJ010000223 DATMXJ010000000
VERSION     DATMXJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14899"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..217
                     /locus_tag="VN377_05950"
     CDS             68..217
                     /locus_tag="VN377_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWR27960.1"
                     /translation="MAIDPICKMTVDEKTAKFTSVYKGKTFYFCAPGCKRTFDKNPEQ
                     YVSIN"
     gene            complement(266..1789)
                     /locus_tag="VN377_05955"
     CDS             complement(266..1789)
                     /locus_tag="VN377_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HWR27961.1"
                     /translation="MGQVVDCFFWDPSIRFDLGLQYVSEEIGNGISLRKPIFTIDALK
                     KIIERIKTARQEYLRTHDIPAVLDAIDKVNALWMDPVYHGRKVARDVLPIVTGFSVEM
                     IESWGFDYFFDIIKKDNLPLAGKLQPNNFRTFSPCGDGLVKAYGKPHVAHSNYEPEVI
                     GHICAGNILGLAAFEMVMDKLIDAATWVKVPTEEPVFGALYAKSIEEVDPLLAYSIAV
                     LPFSSKDALVQDYLFSQSDLVRATGGEQARRNLTALAEKHHVPLAGHWHKFSFMVIAR
                     EFLNRQASEIAELASLDVCAWDQQGCFSPQEIYVETGGSVSPKEFAGLLAKEMEITTR
                     ALPKGTNSGKMQVLDGYYQYFTKETMGEPVKIFPSKNHQWLVIYDERSQICEPSPLFR
                     VIRVKPVTDIMELPQRVKPLASFLQTVGVAIPQDRLLVFADAMGQAGVTNIRTVSGMT
                     LQKAWEPWDGRFPLQELFEHDGIHWVSIDTKDIDAEIKNALKRKQLIVSEDLSEKRN"
     gene            complement(1801..2208)
                     /locus_tag="VN377_05960"
     CDS             complement(1801..2208)
                     /locus_tag="VN377_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWR27962.1"
                     /translation="MVRSILRFPSDEWIKTFKDELNKNKTYQEVAKDWEGDFLFVITP
                     DEELKKETVFYIDLWHGKCKDAYLVKGEKQAKFVFKGPYSNWKKVIRKELDPIRGLIR
                     GMFTVDGDSRVILDQAKAAQELVNTASTIPVVF"
     gene            complement(2174..3559)
                     /locus_tag="VN377_05965"
     CDS             complement(2174..3559)
                     /locus_tag="VN377_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27963.1"
                     /translation="MATAPPTGDSTECLHKLIPPKEQWTPVDTALHAPKTFFLDYNKA
                     QEYMFPALRHTFKHHYENNFLYHRVCEVNGITPEVITSKKDVKKIPLLPDAFFKDYPD
                     GKGFLNWLKTIFTGSLPKPMFKNDNPSHDEIIEEFNKKGTSIMFTSGTSGRFSFIPRN
                     TSSWNRVKYSAMKSVIELMDYNPTDQVILLIPDPRQTNLTIASLFGIAYDLYDPANVH
                     VALDMKITTQFLRMQRDETIGISEKLKAKALSTISPLVQRNSDIKIIHLLEQIEKTGK
                     RVNLAGPPFWLDRILDRMKKEGKSVHLPDSQILTGGGWKAEENKRLPEDSFREKITDI
                     LGIPQERYHDVYAMSECSSVFLSCEGHYKHIPPVVIPLVLDEDLNQLDYEQVGRFAFL
                     DPLPDSYPGFIITGDRVKILDHCPKCQREGPVLDVEVTRMPGFEGRGCAAVMAELMKQ
                     DGEEHTAFPVG"
     gene            complement(3580..3984)
                     /locus_tag="VN377_05970"
     CDS             complement(3580..3984)
                     /locus_tag="VN377_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27964.1"
                     /translation="MVKYLSQEWAEQAKKKVLTELDKARDLKNMNTSLLNVIQNVPPD
                     NKTMYFYIKFIEGNLEELITGSDESIKQKDAEITATGDYTTFVQISSGQMSSAMALLK
                     NRLKMTNKMKALTITKPIDTFNGCIKGIQTEY"
     gene            complement(3978..5918)
                     /locus_tag="VN377_05975"
     CDS             complement(3978..5918)
                     /locus_tag="VN377_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR27965.1"
                     /translation="MTLSDVLEMTKKEPHCGLCKIDFLGIGVCAAGKKHGYVAYWPEG
                     RMELARALAEKEVEPTETLIDIVDSCNLCGVCDRQCYFITQRRPTKAQQALKDYVKAL
                     KPNQFKRVQEDEVVKDLRAIVGADWATNDPIIIASYLRSIIKQKSSGKYYIVMPKTTE
                     EIVAIVKLADKYKIPYLPRGNGTFFGVVLQTLLAEPSTIEQGIIIDLWRMKDIQVSPE
                     TITATIGPGVTSFELQKAAHAHKLRANVAEAESCVCANAQNLGIITTWSNTYGWGTDN
                     FIDAEFIDSTGEVHHLSDKEIPNPYASEQGLLSLSLIPSVIVTQLTLKLHRILDDEEA
                     VFIPFTNLSDALDLAHELATRNIGISLAVLSRKYLTEFVSPTHEVAEDFNYIAEKYLK
                     LNYVVDVICDKHSKKIVEELADITFGHSMIKSLILGSSKLASMKDSSLLKMISEEENP
                     LKAVFGGPLRKHFEKGLASSPKELVKVFDEDLRDFFEKLYSKPEITDPIWLHSFRILP
                     CRMERKHMWMYRGGYMPGDKKTILKVVDALEKPAQLYQIDGAFGFLSYLERGKLAHLE
                     YDYYYDHTDPDARKRVNKAIVESWKRQFTIPGVTPLEFICSKGLHRKEHILYPFPAGL
                     SEEELGHFEELIEMLFGGEDTW"
     gene            6136..7473
                     /locus_tag="VN377_05980"
     CDS             6136..7473
                     /locus_tag="VN377_05980"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWR27966.1"
                     /translation="MKRNMSGLIIMILLGMLLTGCVQKQQEQQPPVKTKEDVSVDIVT
                     LLMENNFTGVYDFFNTSITSQITADDFANIWTQQIGAPNGNITRIVRTRSTNESGYSV
                     VYVTCNFSKVTVLDVKISFNTYEKVVALVVVPTLEAFDYSPPSYVDQLAFTEQNVSFG
                     SEPWKLPATLTIPNGAGPFPAVLLVHGSGPNDRDETYGPNKPFKDIALGLASRGIVVL
                     RYEKRTKQYPKEFAKLQNFTVQDETIADAEAGVNFLYASSIVDHSKIFVLGHSLGGML
                     APRIALQNNHIKGLIIMAGPTRHLEDLILEQSRYLANQSGLNQSAKLAELERLVIKVK
                     NLDMNQSEFILGAPKSYWVDLVTYDPVETAQLLDIPLLILQGDRDYQVTMTDFVRWNE
                     TFNKNSSVTLKIYPSLNHFFIAGTGVPTNAEYLIEGHVAEAVISDIASWITTQ"
     gene            complement(7519..8325)
                     /locus_tag="VN377_05985"
     CDS             complement(7519..8325)
                     /locus_tag="VN377_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27967.1"
                     /translation="MNKKIISLSILSVAIILLTGCTPALASPENKSLAMKTDLVTIEV
                     NHYLGRETKQIRTTVTASDAEQIRIYLTELYKAQERNDIAAIATYEALLNEKGIFGEQ
                     FHRFSSNINGIASIGKTKLSNYFPNIAGENISNSLCFFNAIGEGGMLWWIGLTFIKNL
                     VDAITNVSNPIGAVILLIILLPLIVLTMLLTDLIPFRIFAPTGIISLRNGTVSALGAN
                     GRQKVIVGAESYGVNLSGFTGITINIPPINNHKSFLFVSGFALKAEGQPV"
BASE COUNT         2210 a         1935 c         1857 g         2531 t
ORIGIN      
        1 gtgacggtcg tatcactttc gttgatgtta agaggatatg tgccgccggc acaggaggtg
       61 aaaaatcatg gcgattgatc cgatttgtaa aatgacggtc gatgagaaaa cagcgaaatt
      121 caccagtgtc tacaagggga agacgtttta tttctgtgcc cctgggtgta aacgaacgtt
      181 tgataagaat cctgaacagt acgtctcgat aaactagtga attttcataa aaattatatt
      241 tttgctttag tacctcgata cttgattaat ttcttttttc tgaaagatct tccgaaacaa
      301 tcaactgttt tcgtttcaga gcatttttta tctcagcatc tatgtctttt gtatcaatgc
      361 tgacccaatg gattccatca tgttcaaaga gctcttgtag agggaaccgt ccatcccatg
      421 gttcccaggc tttctgcagc gtcattccac tgaccgtacg gatgttcgtc acaccggcct
      481 gtcccatcgc atcagcaaac acaagcaatc gatcctgcgg aatagcgaca ccaacggtct
      541 gtaagaatga ggcaagtggt ttaactctct gcgggagctc catgatatcg gtaacaggtt
      601 tgacccggat cactcggaac aacggagaag gttcacaaat ctggcttctc tcatcgtaga
      661 tgaccagcca ctgatgattc tttgaaggaa agattttcac cggctccccc atggtctcct
      721 ttgtgaaata ctggtagtat ccatccagca cctgcatttt cccagaattc gtccctttcg
      781 gtaacgcacg ggttgttatt tccatctcct ttgcaagcaa cccggcgaac tccttcggtg
      841 acacgctacc tccggtctcg acgtagatct cctgcgggga gaaacacccc tgctgatccc
      901 acgcgcacac gtcaagggaa gctaactctg cgatctcaga ggcttgtctg ttgagaaatt
      961 cacgggcgat taccatgaaa ctgaacttat gccaatggcc tgcgagagga acatggtgtt
     1021 tctcagcaag tgccgtaaga ttcctacgtg cctgctcccc accggtcgca cggacaagat
     1081 cggattgaga gaataaatag tcctgcacca aggcatcctt gctactgaaa ggaagcaccg
     1141 cgatgctata cgccagcaac gggtccacct cctcgattga ttttgcatag agtgctccaa
     1201 acactggctc ttctgtaggt accttcaccc aggttgcggc atcaataagt ttatccatca
     1261 ccatctcaaa tgcagctaag ccaaggatgt tgcctgcaca gatatgacct atgacttctg
     1321 gctcataatt cgaatgcgcg acatgtggtt tgccatacgc cttcaccagg ccgtcaccgc
     1381 atggcgaaaa cgttcggaaa ttatttggtt gaagtttccc ggcaagaggc aggttatctt
     1441 ttttaatgat gtcgaaaaaa taatcaaaac cccaggattc aatcatctct acagaaaatc
     1501 ctgtcacgat tggtaaaaca tcacgagcga ctttccgtcc atggtacaca gggtccatcc
     1561 acagcgcgtt aaccttatca atcgcatcaa ggaccgcggg gatatcatgc gtacgcagat
     1621 actcctgacg tgcagttttg atcctttcaa tgattttttt taacgcatca atggtgaata
     1681 tgggtttacg cagcgaaatt ccattaccga tctcttcaga gacgtactgt aatcccaggt
     1741 caaatcgtat cgaaggatcc cagaaaaaac aatcaacgac ctgtcccata atcaaccctt
     1801 ttagaataca accgggatcg tacttgcggt gttcaccagc tcctgagctg ccttagcctg
     1861 gtccaggatc acccgagaat caccatccac ggtgaacatc ccccggatca accctcggat
     1921 aggatcgagt tccttccgga tgaccttttt ccagttcgaa tacggtcctt tgaatacaaa
     1981 cttcgcttgc ttctctcctt tcacaagata agcatcctta cattttccat gccatagatc
     2041 aatgtagaat acagtttcct ttttaagctc ctcatcagga gtgatcacaa acaggaaatc
     2101 accttcccag tccttggcga cctcttgata tgtcttgttc ttattgagtt catctttgaa
     2161 ggttttaatc cattcatccg acgggaaacg cagtatgctc ctcaccatcc tgtttcatca
     2221 actctgccat gaccgctgca cagccccgtc cctcaaaccc aggcattcgt gtcacctcga
     2281 catccaagac cggaccttcc cgttgacatt tcgggcagtg atctagaatc ttgacccgat
     2341 cacccgtgat gataaaaccc gggtagctgt cgggtaacgg atcaaggaac gcaaaccgtc
     2401 cgacctgctc atagtcaagc tggttgagat cctcatcaag gactaacggg atcacgaccg
     2461 gggggatatg tttgtaatgc ccttcacagc taagaaacac agaactgcac tcggacatcg
     2521 cgtagacatc gtgatatcgc tcttggggaa tccccaggat atctgttatt ttctcccgga
     2581 atgaatcctc cgggaggcgt ttgttctcct ccgccttcca cccgccacct gtgagaatct
     2641 gactgtccgg cagatgaaca gattttcctt cttttttcat ccgatccaat atccggtcaa
     2701 gccagaatgg tggacctgcg aggttcaccc gcttccctgt tttttcaatc tgctctaaca
     2761 aatgaatgat cttgatatcg gagttacgtt gcaccagagg gctgattgtt gagagtgctt
     2821 ttgcttttaa tttctctgaa atcccgatgg tttcatcccg ctgcatccgt agaaattgtg
     2881 tggtgatctt catgtccagc gcaacatgga cgtttgccgg gtcgtacaaa tcataggcaa
     2941 tcccgaagag acttgcgatt gtcagatttg tttgccgtgg atcagggatg agaaggataa
     3001 cttggtcagt gggattgtaa tccatcaact caataactga tttcatcgca ctgtatttca
     3061 cccggttcca agaactggta ttacgaggta taaaactaaa ccgaccgctt gtaccgcttg
     3121 taaacatgat gctagtgcct tttttgttga actcctcgat gatttcatca tgggaggggt
     3181 tatcgttctt aaacatcggt ttgggcagag atcctgtaaa aatagttttc aaccagttca
     3241 aaaatccttt tccatcaggg taatccttga aaaatgcgtc ggggagcaac ggtattttct
     3301 ttacatcttt cttcgaggtg atcacctcgg gggtaattcc gtttacctcg cagacgcggt
     3361 ggtataggaa gttgttctcg taatgatgtt tgaaggtgtg ccggagggca ggaaacatgt
     3421 attcctgcgc tttattgtaa tcaagaaaga acgtttttgg agcatgcagt gcggtgtcga
     3481 ccggtgtcca ttgctccttt ggaggaatca atttatgtaa acattctgtt gaatcccctg
     3541 ttgggggtgc agtcgccatt ttcttctcct ttaacttcgt tagtattcag tttgaatccc
     3601 tttgatgcaa ccattaaagg tatcgatggg ctttgtaata gttagagcct tcattttatt
     3661 tgtcatcttc aaccggttct tcagcagggc cattgccgag ctcatttgtc cgctgctgat
     3721 ctgaacaaac gttgtataat ccccggtggc agtaatttca gcatccttct gttttatcga
     3781 ttcatcggaa ccggtgatca actcttcgag gttcccttcg atgaatttta tgtaaaaata
     3841 cattgttttg ttatcagggg ggacgttttg aatgacattc aacaacgatg tgttcatgtt
     3901 cttcaaatct cttgccttat caagttcagt taagactttt ttcttggctt gttcagccca
     3961 ttcttgcgaa agatatttta ccatgtgtct tcacctccga agagcatctc tataagttct
     4021 tcaaaatgcc caagttcctc ttccgataac cctgctggga acggatagag aatgtgttct
     4081 ttccgatgga gccctttgct gcagataaac tcaagtggag taactcctgg tattgtgaat
     4141 tgtcttttcc aggattccac gatcgcctta ttgacgcgtt tgcgggcatc tgggtcggta
     4201 tgatcgtagt agtaatcata ttccagatgt gctagcttcc ctcgttcgag gtagctgagg
     4261 aatccgaatg ctccatcgat ttggtacagt tgcgctggtt tttctaatgc atccaccact
     4321 ttgagaatgg tcttcttatc ccctggcatg tacccaccgc ggtacatcca catgtgtttt
     4381 cgttccatgc gacaggggag aatgcggaag gaatgaagcc agatcgggtc tgttatttca
     4441 ggttttgaat acagtttttc gaagaaatcc ctaaggtcct cgtcgaagac ttttacaagt
     4501 tccttaggtg atgatgcaag gcctttttcg aaatgtttcc gcagtggacc tccaaagact
     4561 gcttttagcg ggttttcctc ctcggaaatc atcttcagca ggctgctgtc tttcatggaa
     4621 gcaagttttg atgagccaag gatcaggctt tttatcatag aatgaccaaa ggtgatgtct
     4681 gcaagctcct cgacgatttt cttgctgtgt ttatcacaaa tcacatcaac gacatagttg
     4741 agtttcagat atttttctgc gatatagttg aaatcctccg caacctcatg tgtcggagaa
     4801 acaaactcgg tgagatattt ccgtgagagg actgccagag aaattccgat gtttcttgtt
     4861 gccagctcat gggcaagatc caatgcatcg gagaggttcg taaacgggat aaagactgct
     4921 tcttcgtcat cgaggattcg atggagtttt agcgtcagtt gtgtgacgat cactgaagga
     4981 atgagcgaca ggctcaagag tccttgttcg gatgcgtatg ggtttggtat ctctttatca
     5041 ctcagatgat gtacctcccc tgttgagtcg atgaattccg catcgatgaa gttatcggtc
     5101 ccccatccat aggtgttgct ccaggtggtg atgatgccga ggttctgagc gttcgcacag
     5161 acgcaggatt cagcttccgc gacattggcg cgtaatttat gggcatgtgc tgccttttgt
     5221 agctcaaaag aggtgacccc tggtccgatg gtggccgtta ttgtttcagg actaacttgg
     5281 atgtccttca ttctccagag atctatgatg atcccttgct ctatcgtact tggttcagca
     5341 agcaaggttt gtagtaccac tccaaagaaa gtaccgtttc ctcgcgggag gtacgggatt
     5401 ttatatttat cagcgagctt tactatcgct acgatttcct ctgtcgtttt tggcatgaca
     5461 atatagtatt tacctgatga tttctgcttg atgatcgatc ggagatacga ggcaatgatt
     5521 atagggtcat tggtcgccca gtcagcaccc acgattgcgc ggaggtcttt tacgacctca
     5581 tcttcttgaa cacgtttgaa ttggtttggt tttagtgcct tgacatagtc tttcaaagct
     5641 tgttgggctt ttgtgggtcg tcgttgtgtt ataaaataac attgtcggtc gcaaacacca
     5701 caaagattac acgagtcaac gatatcgata agcgtttctg tcggttcgac ttctttttcc
     5761 gcaagtgctc tagcaagttc cattcgccct tcaggccagt atgcgacata cccatgtttt
     5821 ttcccagctg cacagacacc gatcccgaga aaatctatct tgcataaccc gcaatggggt
     5881 tcttttttcg tcatttctaa gacatcggat agcgtcataa tatccccctg ataatgattt
     5941 tcaatcaagg cttgaaagaa tgccccctct taaagttttt ccataatttt ttatggcaat
     6001 cgattgattc attcaaataa ttctttcgta ttttactaat tataagaggt gttcatcctt
     6061 ttatcacaca gaaaatttat tgaataacct atggttttgc ctccgattaa ttatttcaga
     6121 atgaagcgga gaactatgaa gcgaaatatg tcaggtttaa taattatgat tcttctagga
     6181 atgttgttaa ccggatgtgt gcagaagcag caggagcaac aaccaccggt gaagaccaaa
     6241 gaggatgtct ctgttgatat cgttacactt ctgatggaaa ataactttac cggtgtatat
     6301 gattttttta acacgtcgat aacttcacag atcactgcag atgattttgc gaatatctgg
     6361 acacaacaaa ttggcgctcc aaacggcaac atcacaagaa ttgtgcgaac acgatcgacg
     6421 aatgaatccg gatattctgt cgtctatgtc acctgtaatt tttcaaaagt aactgttctt
     6481 gatgtgaaaa tctcgtttaa tacttatgag aaagtcgttg ctcttgtagt cgttccaaca
     6541 ctagaagcgt ttgattattc gccaccctcg tatgtagatc agcttgcatt tactgaacaa
     6601 aatgtgagtt tcggctcgga accttggaaa ctgcctgcga cgttgacgat tccaaatggg
     6661 gctggcccgt tccctgcggt acttctggtt catggttcag gaccgaatga ccgtgatgaa
     6721 acctacggtc ccaataaacc atttaaagac atcgctttgg gtcttgcttc aagaggtatt
     6781 gtggtattac gctatgagaa gcgaacgaag caatatccca aggaatttgc gaagcttcaa
     6841 aatttcacgg tacaggatga aaccattgca gatgcagagg caggagtgaa tttcttgtat
     6901 gcctcttcga ttgtcgatca ttcaaagatt tttgttctgg gtcatagttt aggagggatg
     6961 ttagctccac gtatagctct ccagaataac catatcaaag gtttaatcat catggctgga
     7021 ccgacccgcc atcttgagga tctgatatta gaacaatcac gatacctagc gaatcagagc
     7081 ggtctgaatc aatctgctaa gcttgctgag cttgagcgac tcgtaataaa agtgaagaat
     7141 ttagatatga accaaagcga attcatcctc ggtgccccga aatcatattg ggtggatctc
     7201 gtcacctatg atcctgtgga aacagcacaa ttattagata ttcctctgct cattctgcaa
     7261 ggagaccggg attatcaagt gaccatgacg gattttgtca gatggaatga aacctttaat
     7321 aaaaactcat ctgtgacctt gaaaatatat ccatctttga atcatttctt catagcgggg
     7381 actggggtgc cgaccaatgc ggagtatcta atcgaaggac atgtcgccga agcagtcatc
     7441 tcagatatag catcctggat aacaactcaa taagaaccag cggtgcatca gtgtctctga
     7501 tttcttatta tgaaaatatt atacgggctg tccttctgct ttgagagcga atccagacac
     7561 aaagagaaac gatttatgat tattgatcgg tggtatgtta atggtaatac cggtaaatcc
     7621 actcaggttt actccatacg attctgcacc gacgatgact ttctgtcggc cgttggctcc
     7681 taaagcggaa accgtcccat ttctcagcga tatgattcca gtgggtgcga agattctaaa
     7741 tggaatcaaa tctgtcaaga gcattgtaag gacaatcagt ggcaataaga tgatcaataa
     7801 aataaccgct ccaatgggat ttgacacatt ggttatcgca tctacgaggt tcttgatgaa
     7861 tgttaatcct atccaccaga gcataccgcc ttcaccaatc gcattgaaaa agcagaggct
     7921 atttgagatg ttctctccag cgatattggg aaaatagtta gagagttttg ttttccctat
     7981 gctggcgatt ccattgatat ttgaagagaa acgatggaat tgttcaccaa aaataccttt
     8041 ttcattcaag agcgcttcgt atgttgcgat cgctgcgatg tcatttcgtt cttgtgcttt
     8101 atacagttca gtaagataaa ttcgaatttg ttcagcgtca cttgccgtaa ccgtggtacg
     8161 gatttgtttt gtttctcttc caagataatg attcacctcg atagtaacaa ggtcggtttt
     8221 cattgcaaga gacttgtttt ctggtgaagc aagcgcaggg gtacatcctg ttaagaggat
     8281 gattgcgact gataagatgc ttaacgatat gattttttta ttcatttttt ttcctctccg
     8341 tttaataata atccgcttcc tctatataat tctttatatg gaagcatcga cgtgtctttt
     8401 cccgaaatta cttcctccta gaaaaaaaca atgatgattc agttccgatg aggaaactga
     8461 atcaacagaa aatacgatgg atcatcacac aaatagacaa aggagaacaa tccacctaca
     8521 gaatcgcaaa gac
//
LOCUS       DATMXJ010000224         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_35774, whole genome shotgun sequence.
ACCESSION   DATMXJ010000224 DATMXJ010000000
VERSION     DATMXJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35774"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="VN377_05990"
     CDS             <1..1753
                     /locus_tag="VN377_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWR27968.1"
                     /translation="LIYMGGDLQKKLIPFFYYALNPDGFLFLGTSETVSEFGDLFTAL
                     DHKLKLFRRRENIYSTQRTASGRFLPPKIAIDATFSHSAEKTDDVVMKLPLREMTERA
                     LLQQYAPIGVLVNGHGDILYFHGHTGVYLEPPQGEAGINNILKMAREGLRHELTKALH
                     KAVSIKEIVYHPGLRIKTNGQFITVNLTVRPMPIGYTAQTEAPLYLVIMEEATTSNHK
                     LAQTNAALHAIAKKDSLDDTGTVTKIMTLKKELRAKEEYLQITNEELETSNEELKSSN
                     EELQSVNEELQSTNEELETSKEELQSVNEELATVNTELQTKVTDLTRINNDMNNLLGG
                     TGIGTIFVDHQLRILRFTSAATQIINLILSDVGRPVGHIVSNLLGYDRIVADTQAVLD
                     SLIPKEVEVQTKTGIWYMLRIQPYRTINNVIEGAVITFVDITTMKKAQEAFEKVHAQT
                     VAILQCTTEGILGLDVQGDHVFVNSAAAKMFGYTPEELVGKNSHNTLHNKHADGSPFP
                     ISTCPIHSTQCDGKSQRGKDYFFRKDGTCFLAEFTKLPTIINSKVTGEIISFRDITDR
                     QPFKIGREKGRRKTIEK"
     gene            1766..>3792
                     /locus_tag="VN377_05995"
     CDS             1766..>3792
                     /locus_tag="VN377_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWR27969.1"
                     /translation="MNNKNNESEQAATLRKKAEEIALKKAVQSSEKLDALSPEEIRQT
                     LHELQVHQIELEMQNEELRRVQANLDEAMERYFDLYNLAPVGYFTINEKGFIIEANLT
                     FASLLGVEKNTLIKKPFTQFILPDDQDVFYNHHKQISKTVTDASQACELRMVKKDGTK
                     FWVHLESTTVLDSDGEQVYRVVMSDITERKKADEILSQSEEKFRKEFSTLFDDAPVGI
                     ALVDKNGIIQEVNNNLLRLLSIRKEELIGSSFVELAPTFGLDITENITDFKNRLIEAP
                     SNKEITFLNKNNSKTTITVQSSTIKSGDEILGILYILNDITDRKKAVEQLKNSEERYR
                     NLIELAPDSIVTLNLKGYFTSCNSAATRIFRYSKDDLIGKHFSKIGVLHVKDVPKYLK
                     VFVSLLQGKSSGIYELTINDKDGTSRIAETHIGLIKENNRISGICAITRDITERKKIE
                     REIIESEEKLKQILDSSPDAITVTDLKGNIIECNQTASKMYGSPKAELIGKNALQFFS
                     PKDRKKAKDNLMKTLEVGFIKNVEYILQTKGGREFPAELSANLIRDASGNPQSFVSIV
                     RDITERKKAEGALKESQEKYRLIAENTNDLIIITNVDKTFRYVSPSIKSLGYTPEELV
                     GQDSFILLHPEDNISITSMLKQLIIGRYKPGTNSRFEYRLRDKSGMYHLYET"
BASE COUNT         1256 a          842 c          828 g          866 t
ORIGIN      
        1 cctaatctat atgggtggag atttacagaa aaaactcatt ccctttttct actacgcctt
       61 aaaccccgat ggttttctct tcctaggtac ctcggagact gtcagcgaat ttggcgatct
      121 ttttaccgcg ctggatcata agctgaagtt atttcggcga agggagaata tttacagcac
      181 ccaacgcacc gcctcaggcc ggtttctacc cccaaagatt gctatagatg cgacattttc
      241 gcattccgct gaaaagacgg acgatgttgt gatgaaactt ccgcttcgtg agatgaccga
      301 acgagcgctt cttcagcagt acgccccgat cggcgtactc gtcaatggcc acggcgacat
      361 cctctatttc catggacaca ccggtgtgta cctcgagcca ccccaaggcg aggctggtat
      421 caacaacatc ttaaagatgg ctcgcgaagg attgcgacat gaactgacca aagcgttaca
      481 caaagccgtt tcaatcaaag aaatcgttta ccacccaggt ttgcgcatta aaacaaacgg
      541 tcaattcatc acggttaatc tgaccgttcg cccaatgccg ataggctaca ctgcacagac
      601 cgaagcaccc ttatatctgg tcattatgga ggaagcaaca acctccaatc acaaactggc
      661 acaaacaaac gctgccttac acgccatcgc aaaaaaagat agtcttgatg atacaggcac
      721 cgtcacgaaa attatgaccc ttaagaagga attgcgagcc aaagaagaat atcttcagat
      781 caccaacgag gaattggaaa cctccaacga ggagctcaaa tcctcgaacg aggagttgca
      841 gtcggtcaac gaagaattgc aatccaccaa cgaggagctg gaaacctcca aagaggaact
      901 gcagtcggtc aacgaagaat tagctacggt caacactgaa ctgcaaacca aggtgacgga
      961 cctgacgcgg atcaacaatg acatgaacaa cctattaggt ggtaccggta tcggcaccat
     1021 ttttgtggac catcaacttc gcatcctacg ctttacctcc gcggccactc agatcatcaa
     1081 cctgatcctg agcgatgtag gacggcccgt aggccacatc gtctctaacc tgttgggcta
     1141 cgaccgtatt gtggcagaca ctcaggcggt gctggacagc ttgattccca aagaggtgga
     1201 agtgcagacc aagacaggca tatggtacat gctgcgcata caaccctacc ggactatcaa
     1261 taatgtcatc gaaggcgcgg tgatcacctt tgttgacatc acgacaatga agaaggcgca
     1321 agaagcgttt gaaaaagttc atgctcaaac agttgctatc ctgcaatgca cgactgaggg
     1381 aatcctcggt ttagatgtgc aaggtgatca tgtctttgtc aactctgcag ccgcgaaaat
     1441 gtttggatat acaccagaag agcttgtcgg aaaaaacagc cacaacacct tgcacaataa
     1501 acacgctgac ggttcaccat ttcccataag cacatgtccg atccattcca ctcaatgcga
     1561 cggtaaatca cagcggggga aagattattt tttcagaaaa gatgggacat gttttttagc
     1621 tgaatttaca aaacttccga ccattatcaa ttccaaagta actggcgaaa tcatcagctt
     1681 tcgagatatc accgatcgcc aacctttcaa gatcggaagg gaaaaggggc gaagaaagac
     1741 tatcgaaaaa taacggagac gcacaatgaa taacaagaat aacgaatccg aacaagctgc
     1801 cacactgcgg aagaaggccg aagaaatcgc tctgaaaaaa gcggtccagt cgtcggaaaa
     1861 actggatgct ctgtcaccgg aagaaatacg gcagacgctc cacgagctgc aggtgcacca
     1921 gatcgagctt gagatgcaga acgaggaatt gagacgagtg caggcgaacc tagacgaagc
     1981 gatggagcgc tatttcgatc tgtataacct tgcaccggtt ggttatttca ccattaacga
     2041 aaaggggttc atcatcgagg caaatcttac gtttgctagc cttttaggtg ttgagaaaaa
     2101 caccctgatc aaaaagccat tcacacaatt catccttcct gatgaccagg acgttttcta
     2161 caatcaccat aaacaaatct caaagacagt caccgacgct tcgcaggcgt gcgagttgag
     2221 gatggtgaaa aaagacggaa cgaaattctg ggtacatctg gagagcacca ccgtgctgga
     2281 tagcgacgga gagcaggtgt accgtgtcgt gatgagcgac atcaccgaac gcaagaaagc
     2341 ggatgaaatt ctcagtcaaa gtgaagaaaa atttagaaaa gaattcagca cattgttcga
     2401 tgacgctcca gtaggcattg cattggtgga caaaaatgga attatacaag aagtaaataa
     2461 taatctgctg cggctattgt caatacgaaa agaagaatta atcgggagca gctttgttga
     2521 actagcccct acttttggtt tggatataac agaaaatatc acggatttca agaacagact
     2581 cattgaagct ccatcaaata aagaaataac attcctcaat aagaataaca gcaaaacaac
     2641 gataacagta cagagttcga ccataaagag cggcgatgaa atacttggca ttctgtacat
     2701 tttaaatgac attactgatc ggaaaaaagc agtggaacaa ttaaaaaatt cagaggagcg
     2761 ctataggaac ctcattgagc ttgctcctga ttcaattgtg accttgaatc taaaaggata
     2821 tttcacctcc tgtaattctg cagcaacacg catttttagg tattctaaag atgatttgat
     2881 aggaaaacat ttctcaaaaa taggtgttct gcatgtaaaa gacgtcccga aatacctcaa
     2941 agtgtttgtt tcattgctgc agggaaagag ttcaggtatc tatgaactta cgataaatga
     3001 taaagatgga acctctcgta ttgctgaaac acatattggt cttatcaagg agaataatag
     3061 gatttcaggt atttgtgcaa ttacgcgcga tatcacagag cgtaagaaga tagaaagaga
     3121 aattatcgaa agtgaagaaa aactaaagca aatactcgat tcatcccctg acgcaatcac
     3181 ggtcacagat ttaaagggaa acatcatcga atgcaaccaa acagcatcaa agatgtatgg
     3241 ttcacccaag gcagaattga tcggtaaaaa cgcattacaa tttttttctc ctaaagatcg
     3301 gaaaaaagcg aaagataatt taatgaaaac attagaagta gggttcataa aaaatgtaga
     3361 atacattcta caaacgaagg gggggcggga gtttccagct gaactctcag caaacctaat
     3421 cagagacgcg tccggtaatc cacaatcttt cgtttctata gtaagagata tcaccgagcg
     3481 taagaaggcg gaaggagcgt tgaaagagag ccaagaaaag tatcgtctga ttgcagagaa
     3541 cacaaatgat ctgataatta ttaccaacgt tgacaaaact tttcgttatg tgagcccctc
     3601 gattaaatcg ttagggtata ctcctgagga actggttgga caagatagct tcatcctact
     3661 acatcctgaa gataacataa gtattacatc catgttaaaa caattaatca ttggaagata
     3721 taaaccagga acgaactctc gcttcgaata ccgcctccgg gataaatcag gcatgtatca
     3781 tctctatgaa ac
//
LOCUS       DATMXJ010000225         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_29555, whole genome shotgun sequence.
ACCESSION   DATMXJ010000225 DATMXJ010000000
VERSION     DATMXJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29555"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..523)
                     /locus_tag="VN377_06000"
     CDS             complement(221..523)
                     /locus_tag="VN377_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27970.1"
                     /translation="MVYSILQSDFRCQYLQTCLNMFPDDIPEFFRLLNQNQAIYEFVT
                     KPTYHYCLSNENHVRCVRYEIKQEGKEPPPGLTPDGQNVNITDSIFKKKIIFEQAP"
     gene            1032..2795
                     /locus_tag="VN377_06005"
     CDS             1032..2795
                     /locus_tag="VN377_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWR27971.1"
                     /translation="MKKTFIILVMVLLVFPVSLGQSYDNDDSRSAQNNSTDTSNVEIP
                     VPLLRFKTTFMVPMRDGIQLATDVYRPIFRPTPHGAMLIRTPYNKDSIAIVGLLGILR
                     GWPVVIQDMRGRYASKGIDTGFRSDSADGADTLAWIANQSWSNGKIVTYGLSALGIVQ
                     YCLAGAHPPNLTCQYVQVATPNLYKHGVYQGGEFRKNLVEDWLSDQESDYLLEEIKNN
                     ENFTLGYWGNVTLDDNWEDVNVPAIHIGGWYDIFSQGTIEGYLGYQHSAGPGALGKSK
                     LIMGPWTHTGFLSRTQGELTYPRNSVDRFSLRMFRDLVRLYVMNDTDKYSAWPTVSYY
                     VIGDVDDADAPGNEWRYADDWPVSADEVPWYFQTDGLLTTETPRSSEPLMYLYDPVNP
                     VPTVGGKNLYSPAGPYDQSSVENRSDVLVFTSDILENPFEATGPVKARLFVSSDCPDT
                     DFTVKLTDVYPDGRSMFITDGILRMRNRNGQDHWEFMEPGSIYEVEVDLWSTSYIWDA
                     GHRIRVEVSSSNYPRFLNNPNTIDGIGKNTSYSIAQNVLYVDSTHPSCLLLPEPNQEY
                     QEKMFKPPNIFPSRFISALFL"
     gene            2973..>3738
                     /locus_tag="VN377_06010"
     CDS             2973..>3738
                     /locus_tag="VN377_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWR27972.1"
                     /translation="MLPVAQVGSSNAVRNTIIKNKTFDSETLSVSSYDPVYSLTPDWV
                     SGSPDYSTGGAFADFNNDGWLDFIVSDGNDMGQGHVRVYMNDGDGHLPTTASWESADV
                     AYNGHLDVADINGDGWSDVAVSYLGTGSSLGPIARVYMNDNGVLSSSPSWTSNIIGNA
                     FGVDFGDMNNDGRPDLAIATGWSYDSYHYHTYVYLNTDGSYGSSPSWQSADQNIYMNV
                     LWVDADDDGWLDLAGIADSTQTQIYRNINGVLEATAS"
BASE COUNT         1019 a          806 c          876 g         1037 t
ORIGIN      
        1 cataagagca agggaaaaaa accaatagag aatcccaata atatttaaga gaaaacaacc
       61 taaatttccc aaactaagtt tcccatactc aaatgacaca gtaccgaaaa atcatccaga
      121 gaatcctttc tcattttcca atgctttcag ggtttacaga aaagctaaaa acatgcgcta
      181 atcatctttt ataaaaaaag aagtatgaga tgtgatcagg ttagggggct tgttcaaata
      241 ttatcttctt tttgaagatt gagtcagtga tattgacgtt ctgtccatct ggtgttaatc
      301 ctggtggtgg ttctttacct tcttgtttta tttcataccg aacacatctg acatggttct
      361 catttgatag gcagtaatga taagttggtt tggtgacaaa ctcatagatg gcttgattct
      421 gattcaataa tctgaaaaat tcagggatgt catcagggaa catattcaga catgtttgaa
      481 gatattgaca gcgaaaatca ctttgaagaa tactatagac catacatttc tgatatctct
      541 ccgattcaca cgaagaaaaa tctttgtatt gaataatagc gggatatatt cgtatacaat
      601 ctctggtaaa atcgatataa tacggacaag tcataactac tcacaaccta taaaatcaaa
      661 tcttttatga caataaaaat gttttgtagt cagcgaccaa tatctcctgg agtaattcca
      721 taaccagtgg tgttgtcatt ctaggaaaag aaacctgtgg ttcgatagcg acgattgata
      781 gtggagatac cgaacagttt aaatcaattc tcaatctttg gactgggaaa atcgacaatc
      841 atcatgcatg tcgtctgtga tgaggatatt gaagcacgaa aacagcgaat gggttcgtgt
      901 ttctcttctt cgtcgcaggg ttgaaataat tatttccgtc ctcatttcgt tttttatttt
      961 ttttattccg aaatgagcaa aagaaaggtt taaatgaatt cgttacataa gaggtttgta
     1021 ggaggtaact tatgaagaaa acattcataa tactcgtgat ggtattgctc gtttttcctg
     1081 tgagccttgg tcagagttac gataacgatg attcgaggag tgctcaaaac aatagtacgg
     1141 atacatcaaa tgtagaaatc cctgtccctc ttctacgttt caaaacaacc tttatggttc
     1201 cgatgcgcga tggaattcag ctagctacgg acgtctatcg accgattttc cggccgaccc
     1261 cgcatggggc gatgttgatc agaacaccgt acaataaaga ttccattgcg attgttggtc
     1321 tccttgggat ccttcgtgga tggcccgtag tcatccagga tatgcgtgga cgctacgcat
     1381 caaagggcat cgacactggt tttagaagcg atagcgccga tggtgctgat accctagcct
     1441 ggattgcgaa tcaatcctgg tccaatggga aaatcgttac ctatggatta tccgcgttag
     1501 gcatcgtcca gtactgtctt gctggtgctc atccccctaa cctcacctgt caatacgtcc
     1561 aagtagcaac accaaacctc tacaaacacg gggtgtatca aggcggggag ttccgaaaga
     1621 acctggtgga ggactggctc agtgaccaag agagtgacta tctcttagag gagataaaga
     1681 acaacgagaa cttcacgctc ggatactggg ggaatgtgac ccttgatgat aactgggagg
     1741 atgtcaacgt ccctgcgatc catatcggag gttggtatga tatcttcagc cagggaacca
     1801 tcgaaggata tctcgggtat caacattctg caggaccagg ggctcttgga aaatcaaaac
     1861 tgattatggg cccatggacg cataccggtt ttctctcacg tacacaaggt gagctgacct
     1921 acccgcgcaa ttcagttgat cgattttcct tacgaatgtt ccgggatttg gtccgattgt
     1981 atgtcatgaa tgacaccgat aaatattctg catggccgac ggtctcttat tatgtcatag
     2041 gtgatgtcga tgatgctgac gccccaggga acgaatggcg gtacgcggat gactggccgg
     2101 tatcagcgga tgaggttcct tggtacttcc agaccgatgg tttgttaacc acagagaccc
     2161 caaggagctc tgagccgttg atgtatctct atgaccctgt gaatcccgtg ccgacggtcg
     2221 ggggaaagaa tctctattct cctgcagggc cctatgatca atcatcggtg gagaaccgat
     2281 ctgatgtgct cgttttcacc agcgatatat tagaaaatcc ttttgaggcc acaggaccgg
     2341 tgaaggcacg tttgtttgtt tcctcggatt gtcctgatac tgattttacc gtgaaactca
     2401 ccgatgtgta cccagatggt cggtcgatgt tcatcaccga tgggattctg cggatgcgaa
     2461 accgaaacgg gcaggaccat tgggaattca tggaaccggg gagcatctac gaagtcgagg
     2521 ttgatctgtg gagcacgtcc tatatctggg atgctggtca tcgtatccgc gtggaggtct
     2581 cctcgtcgaa ctacccgcgt tttctgaata atcctaacac cattgatggg attggtaaga
     2641 acacatcgta ctctattgct cagaatgtct tgtatgtcga ctcaacgcat ccttcctgtc
     2701 ttctcctgcc tgaaccaaac caggagtacc aggagaaaat gttcaaacca ccaaatattt
     2761 ttccatcacg atttatctct gcgttgtttt tgtagaacat catcaagata atcctaagag
     2821 ttctatgaaa taaatgggag atgaacagac gcatccagtg gatatatttt taaagggaac
     2881 ggctcttatt accatgtaga aaggggatac tcctgaagag gtatggcgta ttacagaaaa
     2941 gtagcattgt tttattcata tttgtattga tcgtgttgcc ggtcgctcaa gttggttctt
     3001 cgaatgccgt ccgaaacacg atcataaaaa acaagacgtt tgacagcgaa actctttctg
     3061 tatcatcata tgatcccgtg tattcactga caccagactg ggtctcaggc tcaccggatt
     3121 atagcaccgg tggggcgttc gctgatttta acaacgatgg ctggcttgat tttatcgtct
     3181 ccgatggtaa cgacatggga caaggacatg tcagagtcta catgaacgac ggggatggtc
     3241 atcttccgac cacggcaagc tgggagtcag cggatgtcgc ctataacgga catcttgatg
     3301 tcgcagacat caatggtgat ggctggtctg atgtcgccgt ctcctatcta ggcactggtt
     3361 cttcgcttgg tccgatcgcc cgggtgtaca tgaacgacaa tggggttctc tcctcatccc
     3421 ccagttggac ttcgaatatc atcgggaacg cgttcggagt ggatttcggg gatatgaaca
     3481 atgatggcag gcctgacctt gcgatcgcga ccggatggtc ttacgactcc tatcattacc
     3541 atacctatgt gtatctcaac actgatggat cctatgggtc gtcaccgagt tggcagtccg
     3601 cggaccagaa tatctatatg aacgtcctct gggtcgatgc tgatgatgac ggctggcttg
     3661 atctcgcagg catcgcagat agtactcaga cgcagatcta ccggaacatc aatggagtgt
     3721 tagaggccac cgcaagct
//
LOCUS       DATMXJ010000226         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_20177, whole genome shotgun sequence.
ACCESSION   DATMXJ010000226 DATMXJ010000000
VERSION     DATMXJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20177"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1300)
                     /locus_tag="VN377_06015"
     CDS             complement(89..1300)
                     /locus_tag="VN377_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HWR27973.1"
                     /translation="MKLRQITARQKTVLFLGIFLGIIAIILVGYLLAPTLVYDQWIWK
                     YYWGPVVADATGHSVSYHGVAAQEGYTIISELTYGIILVFALFGLYKLLKKLDIKIDW
                     YFCLALLPYILFGPVTRVLEDTNFFTEPYVYWFISPLIYFQTTFYVLIFLLIGYSLSK
                     KAMSQRKTLSILFLLFLFIDGFYTVLWSLGVHFGVSNIEPVFFYGLSFLAFLPLLYPL
                     IKKQTMTVNTVVFSGGLLVSLPCFFLIGRWILGDQWSLSYGTYVNVLFLVIGLVSLIV
                     SAVYLIAWIYRKHSQIAAYRNPLNLSMLAGHLIDGITSYVSIYDPLHMGLPSYIEKHP
                     ASNSLMEAWPPLFPIVKFLLIILVIYVFDVMYKKELERYSRLINLLKIGIFILGVSPG
                     LRDLLRVTMGV"
     gene            1424..2527
                     /locus_tag="VN377_06020"
     CDS             1424..2527
                     /locus_tag="VN377_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWR27974.1"
                     /translation="MKKINTILCITLLLLPIVSVSALKNNTIEPIAVLPAAFSWRDIN
                     GTDYTTPIRDQSPAPTCEAFAICAVLETKMQYQLKERYNPDLSENHLFFYAGGTIEKG
                     YVSIVDAAQYLMEVGVPDEGCFPDPHRAFDYSFESLPGWENRTVKITEWGWVDHNITA
                     MKQALTDHGPLIICIHFWQDFFYYKGGVYKHHTGEHAGGHVVTIVGYDDSKSCWIVKN
                     SWGTKWGEKGWFKMAYDADMIAGWYGPGTGVMYIEGVYGNLKPDAPKAYIEKPDYSHS
                     YFFGIGLPTLLKNLSIQKAAARIFGPLTVRVTAENTNSVEFFIDDASKSVDTEAPFSW
                     KLDTTSGLHTLMVKATNDHNISSLDIQDIYVMF"
     gene            2649..3743
                     /locus_tag="VN377_06025"
     CDS             2649..3743
                     /locus_tag="VN377_06025"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HWR27975.1"
                     /translation="MERFTKFKTMIFPREVIVGHNTTDQIKGICNHLSPSNNVLLVCD
                     KNTKKISGDRIEKILAGAGYDVNMVVLSLPLIKRDVLPAWPTIREVNTVVKEAKRTGS
                     DFLLGVGGGSVIDVTKCATYELSTNFVSVPTSASHDGMASPRAALKDKKGSISKTAVS
                     PMAVLADTAVIMKAPYRMLASGCADVISNLTAVKDWELAHRLKDEEFSTFAASLSTTS
                     SQLLLNKAEDIRTGLEEAVWQAIKCMVVSGVAMSVAGNTRPASGAEHMFSHMLDSISP
                     GKALHGEQCGVGSIMMMYLHGGDWQQIRDALKMIGAPTTAKELNIPKKKIIQALMGAQ
                     KIRPDRYTILGELGLTEDAAKKLAKITGVI"
     gene            3743..4171
                     /locus_tag="VN377_06030"
     CDS             3743..4171
                     /locus_tag="VN377_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HWR27976.1"
                     /translation="MVLVTLVGEQLAVEGQEFVYLGSNSECRNCQLKTVCFNLKPGRN
                     YRITKLRDKFHDCNIHEGKVIVVEVEELPLTVAVTKEPSEGATTTIEKKECKNIGCDS
                     FEICTTTALQNGKTYTVKKVYEKLTCPKNYELYKVDVTDS"
     gene            complement(4224..5393)
                     /gene="metK"
                     /locus_tag="VN377_06035"
     CDS             complement(4224..5393)
                     /gene="metK"
                     /locus_tag="VN377_06035"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWR27977.1"
                     /translation="MAKRKSIISSESVTEGHPDKMCDLISDAILDEALRQDKHSRVAV
                     ETGTKNGGVMVFGEMTTDAYIDIPHIVRETIKGIGYTKSEYGIEWETCSVWVQITEQS
                     PDISQGVTVGKGLHKEQGAGDQGMMYGYACNETKEFMPLPILLAHKLTKRLSEMRKTN
                     RISYLRPDGKSQVSIEYDEKGKPLRAHTIVVSTQHDGGVAHERIRKDVIDTVIKPVCN
                     EWIDKDTIFHVNPTGAFVRGGPYADAGLTGRKIIVDTYGGVGRHGGGAFSGKDPTKVD
                     RSAAYATRYIAKNIVASGIAEKCEIQFAYAIGVAEPVSINIDCFGTNKISLDAIEKLI
                     REFFPIRPADIINQLDLRRPIFKSTAAYGHFGREEPDFTWEKTDKAKLLKTEAKI"
BASE COUNT         1702 a         1243 c         1234 g         1406 t
ORIGIN      
        1 atccggatgt cgtctcatac tctccgcaga aaagacaatg ctggtttata attctgttgc
       61 attctgttga aaatgcaagg cggtggtctt atacgcccat cgtcacccgt aacagatcac
      121 gcaatcccgg ggagacgccg aggataaaaa taccgatttt cagcaggttg attaaccgtg
      181 aatatcgctc tagttctttt ttgtacatga catcaaagac ataaatcacc aggatgatga
      241 gaaggaattt cacaatggga aataacggtg gccatgcttc catcaggcta tttgaagcag
      301 ggtgtttctc gatataggat ggtaacccca tatggagagg atcataaata ctcacataac
      361 tggtgatccc atcaatcaga tgtcctgcaa gcatcgagag gtttaaggga tttctgtacg
      421 ctgcgatttg gctatgtttt cggtatatcc atgcgatcag gtacactgct gaaacaatga
      481 gactgacaag accaatgaca aggaataaga cattgacata ggtaccatat gacaggctcc
      541 actgatcacc caggatccat cgtccaatca ggaaaaaaca ggggagggag acaagcaatc
      601 caccggagaa caccactgtg tttaccgtca tcgtttgttt ttttatcaaa ggatacagaa
      661 gaggtagaaa cgcgaggaaa gatagaccgt aaaagaaaac aggttcgatg ttagagacac
      721 cgaaatggac tcctagagac caaagcacag tatagaaacc atcaataaag aggaacaaaa
      781 ggaaaagaat ggagagtgtt ttcctttgag acatcgcttt ttttgaaaga gagtacccta
      841 tgagaagaaa aatcaatacg tagaacgtcg tctgaaagta aatcaacggt gagatgaacc
      901 aatacacata cggctcagta aaaaagttgg tgtcttccaa aacacgggtc actgggccaa
      961 acaggatata gggtagtaat gctaagcaga aataccagtc gatcttgatg tcaagtttct
     1021 ttagcaattt gtataacccg aagagggcaa aaacaaggat aattccgtag gtaagctcag
     1081 agataatggt gtatccttcc tgtgcagcca caccatggta agagaccgaa tgacctgtcg
     1141 catcagcaac cacaggcccc caataatact tccagatcca ttggtcatac acaagggtcg
     1201 gagcaaggag atatccgacg agtatgattg caatgattcc aagaaaaata ccaaggaaca
     1261 gcacagtttt ctgacgtgca gtaatctgtc gaagtttcat tcgcggaagg ctaagaaaat
     1321 gaccatatat taatttaccc cgtgccaaag ggaaaatttc gtccattaac gtaagaacaa
     1381 atattataaa ggtaaaataa tatgtatatc gtaggggaac attatgaaga aaattaatac
     1441 gattctttgt atcactctct tattacttcc aattgtttca gtgagcgcat taaaaaacaa
     1501 caccattgaa ccgatagctg tgttacccgc agcgttcagc tggcgtgaca tcaacggaac
     1561 agattatacc actccaatta gagatcagtc accagcgcct acctgtgaag cgtttgccat
     1621 ctgtgcagtc cttgagacaa aaatgcaata ccagctcaaa gagcgttaca acccggatct
     1681 ttccgagaat catctatttt tctatgcggg aggaaccatt gagaaaggct atgtcagcat
     1741 cgttgatgca gcacagtatc tcatggaagt tggtgtgcct gatgaaggat gcttccctga
     1801 ccctcaccgg gcattcgatt attccttcga aagcctcccc gggtgggaga accggaccgt
     1861 gaaaatcacg gaatggggat gggtcgacca taacatcacg gccatgaaac aggcactgac
     1921 agaccatgga ccactcatca tctgtatcca tttctggcaa gatttcttct attacaaggg
     1981 tggagtctac aaacaccata ccggagagca tgcgggaggc catgtcgtca ccatcgttgg
     2041 atatgacgat agcaagagct gctggatagt caaaaacagc tggggcacaa aatggggaga
     2101 aaaaggctgg ttcaaaatgg cctacgatgc agacatgata gcaggatggt atggaccggg
     2161 aaccggtgtg atgtacatcg aaggagtcta tgggaacctc aaaccagatg ccccaaaagc
     2221 gtacatagaa aaacctgatt actctcacag ctatttcttt gggatcggac taccgacgct
     2281 tctaaaaaat ctatctatcc agaaagctgc agcccgaatc tttggaccac tcaccgttcg
     2341 agtgacagca gaaaacacca attcagtgga attctttatc gatgacgcat caaaatctgt
     2401 cgataccgaa gcaccattct cctggaaact tgacacaaca tcgggactcc ataccctcat
     2461 ggtaaaagcg acgaatgatc ataatatctc ctccctggac atacaagata tctacgtgat
     2521 gttctaatac cacccattca cttacgtata attgtttata tgtttcgggt ttttggggat
     2581 tgtctcttta taaaggatta ccgcaaagaa aagataataa acctgtagtc tactcccctg
     2641 aatccaatat ggagcgattt accaaattca aaaccatgat tttcccaagg gaagtcatcg
     2701 tcggccataa tacgaccgat cagatcaaag gaatctgcaa ccacctttca ccaagtaaca
     2761 atgtgttgct tgtctgtgat aagaacacca agaaaatatc tggtgatcgc attgaaaaga
     2821 ttctagctgg cgccgggtac gatgtcaaca tggtcgtcct ctccttaccc ttgataaaac
     2881 gagatgtgct tcctgcatgg ccaacgatac gagaagtcaa taccgtcgta aaagaagcaa
     2941 aaagaacagg ttcggatttc cttctcggag tcggtggagg ctctgtcatt gacgtcacaa
     3001 aatgcgccac ctatgaactc tcaacaaatt ttgtcagtgt cccaacctca gcgtcacacg
     3061 atggaatggc atcaccacgc gctgcgctca aagacaagaa aggatcaata tcgaaaaccg
     3121 cagtatcacc aatggcagtt ctcgcagaca ccgcagtcat catgaaagcc ccctatcgca
     3181 tgcttgcatc aggatgcgcg gatgtgatat caaatctgac cgcggtgaaa gactgggagc
     3241 tggctcatcg tttaaaggac gaagagttca gtacctttgc tgcgtcgctc tccacgacat
     3301 cgtctcaact gttgctgaac aaggcggagg atatccgtac aggccttgaa gaagcagtct
     3361 ggcaggccat aaaatgtatg gtcgtatcag gggtcgcgat gagtgttgcg ggaaacacac
     3421 gacccgcatc tggtgctgaa catatgtttt cccatatgct tgattccatc tcaccaggga
     3481 aagcattaca cggggaacaa tgcggggtcg gttccatcat gatgatgtac ctccatggag
     3541 gagactggca acaaatacgc gatgcgttaa aaatgattgg agcaccgacg acggcaaaag
     3601 aacttaacat cccgaagaaa aaaataatcc aggcgctcat gggagcgcag aaaatacgtc
     3661 ctgacagata cactattctt ggtgagctcg gattaacaga ggatgctgca aagaagctgg
     3721 cgaaaataac tggagtgatc taatggtcct tgtcacgctc gtcggtgaac aactggccgt
     3781 tgaaggacaa gaatttgtct accttgggtc gaacagcgag tgtagaaatt gtcaactaaa
     3841 aaccgtctgc ttcaatctga agccagggag aaactatcgt atcaccaaat tacgggataa
     3901 attccatgac tgtaacatcc atgagggaaa agtgatcgtc gtagaagtcg aagaactccc
     3961 gttaaccgtt gcagtcacca aagaaccctc cgagggagca acgacaacaa tcgaaaagaa
     4021 agagtgtaag aacatcgggt gcgattcatt tgagatatgt acgaccacag cacttcaaaa
     4081 cggcaaaacc tataccgtta agaaggtgta cgaaaaatta acctgcccaa aaaattacga
     4141 gttgtataaa gtagatgtta cagattcata gctaaaaaaa tattgtactt gtttttctga
     4201 taaaaagaaa ataatgagga agattagatc ttcgcctcag tttttaacaa ttttgcctta
     4261 tcggtcttct cccaggtgaa atcaggttcc tctcgtccaa agtgaccgta agcagcagtg
     4321 ctcttgaaaa tgggtcttcg taaatcaagt tgattgatga tatcagcagg tcgtattggg
     4381 aaaaattccc tgatgagttt ttcgatagca tcaagagaga tcttgtttgt accgaaacag
     4441 tcgatattga tggacaccgg ttctgccaca ccaatggcgt aggcgaattg gatttcacat
     4501 ttttcagcga ttcctgaggc gacgatgttt tttgcgatat accgtgtcgc atacgcagca
     4561 ctacggtcga cttttgtagg gtcttttccg gagaaggcac caccaccatg cctaccaacc
     4621 cctccatatg tatcgacaat aatcttcctt ccagttaaac cagcatctgc gtatggacca
     4681 ccacggacaa atgctcctgt cgggttgaca tggaatatag tgtccttatc aatccactcg
     4741 ttacagacag gttttatgac agtgtcaatg acatcttttc gtatccgttc atgggcaacg
     4801 cctccatcgt gctgggtgga aacaacaatc gtatgagctc ggagaggctt gcctttttca
     4861 tcgtattcaa tggaaacctg tgatttccca tcaggtcgca ggtaggagat ccgattggtt
     4921 ttcctcatct ctgataatcg cttggtcagt ttatgcgcaa gaaggatcgg taatggcatg
     4981 aattctttgg tttcattaca ggcgtatcca tacatcattc cctgatcgcc tgctccttgt
     5041 tccttatgta atccttttcc taccgtgaca ccttgagaga tgtcagggct ttgctcagtt
     5101 atctgaaccc acaccgagca ggtttcccat tcgataccat attcggattt cgtgtatccg
     5161 atacctttga tggtctcacg gacgatatga ggaatatcaa tgtaggcgtc cgtggtcatt
     5221 tcaccaaaga ccatgacccc tccattcttc gttccggtct caacagcaac tcttgagtgt
     5281 ttatcctgtc ggagtgcttc atcaaggatc gcatcagata tcaaatcgca cattttatca
     5341 ggatgtcctt cagtaaccga ttcggaactt atgatactct ttcgttttgc catatattca
     5401 cccagttaaa aacctctcat tttctgaatt ccagaaccag agggaattta ttaagtttat
     5461 caaaaaggaa ctgccggtga aaaaaagaaa aaaaataagg agatatttac cctatttttc
     5521 tcgttgtcgt ccacatgtcg tcttcgatgg tattgtagat atcatcatca gagacgttct
     5581 ttggt
//
LOCUS       DATMXJ010000227         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_77799, whole genome shotgun sequence.
ACCESSION   DATMXJ010000227 DATMXJ010000000
VERSION     DATMXJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77799"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /gene="ligA"
                     /locus_tag="VN377_06040"
     CDS             complement(<1..990)
                     /gene="ligA"
                     /locus_tag="VN377_06040"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWR27978.1"
                     /translation="MNKNDAKKQIQKLRDDINYHNYQYYVENNPVISDYEYDMLLKKL
                     ETLETQYPDLITSDSPTQRIGGEPQKGFTTVEHKIPMLSLDNAYSYDELREFDERVKK
                     NITDVEYICEPKIDGASIALIYENGIFIRGATRGDGIKGDDITSNLRTIRSIPLRLQG
                     SELQNAEVRGEVYFPISSFKKFNKEQEKKAEQVFANPRNAAAGSLRQLDPRIVASRPL
                     DTYLYYISHSDKDFKSQEKALETLRTAGFRVNPLIEKASSIEDAISYCKTLETKRETL
                     DYEIDGVVLKVNSFTQQRELGSTIKHPRWAIAFKFTAKQATTRLKDIVVQVGRT"
     gene            complement(995..2710)
                     /gene="polX"
                     /locus_tag="VN377_06045"
     CDS             complement(995..2710)
                     /gene="polX"
                     /locus_tag="VN377_06045"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWR27979.1"
                     /translation="MKNQFVADTLYLIADLLDLKGEMFFKTRAYRIAAQTIEALDEDI
                     EKIIQQGTVESIPGVGEALAKKIIELVQTGKLEYLERLKKEVPTGLIDLLGIPGLGPR
                     KVAALYKNRGITSIQDLQKAASNGKLRTLKGFGEITERNILRGIQLLEKTSGRVLLNV
                     AYEDGNRYLTYLKKCKKIEKINIAGSLRRMKETIGDLDILASSNDPSVVMDCFIQYPE
                     VAEILAKGPTKSSVRLADNLQVDLRVVEAKSYGAALQYFTGSKDHNVTLRGYAIKKGY
                     KLNEYGLFDKKTEKYISGRTEEEIYKKIGMPYIEPELRENRGEFEAARNNTLPTLVKY
                     EEIAGDFHVHSIWSDGSDSIETIAAAAHKRGYRFIGVTDHSQSLKIANGLTEDRVQKK
                     IQEIKKVEKKFPELRIFCGTECDIKTDGTLDYSKKTLQQFDFVYIAIHTAFKMDKETM
                     TKRIIKAMDNEQVNFLAHPTGRLIGKRDPYEVDIEQIIDTAKETDTRLEINSFPDRLD
                     LDDVHIKQAKDHDVRFVLGTDSHSINHLDFMRFGIATARRGWLEKKDILNTSTVQEIE
                     KIIGL"
     gene            complement(2727..3386)
                     /locus_tag="VN377_06050"
     CDS             complement(2727..3386)
                     /locus_tag="VN377_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HWR27980.1"
                     /translation="MKPYLMQTLKELALLGAVHNKIELSSHELADQLHTSQQTASRYL
                     LELDTQEMITRELGVKKQLIQITGGGAEALENEYHSYKQIFDLTNKVVFKGKLVSGMG
                     EGKYYTGHKGYADQFEKRLGFVAYPGTLNVEIEYIERNKIRLLRSYGGIEIDEFQAEN
                     RTFGSVICFKATINGRKGAIVLPKRSHYATVLECISPDNLRETLGLHDGDAVEIVVHL
                     Q"
     gene            complement(3412..3639)
                     /locus_tag="VN377_06055"
     CDS             complement(3412..3639)
                     /locus_tag="VN377_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27981.1"
                     /translation="MDEGFILANKFRRVIFDELVAGENDIKRIAKKNRMIPRVAQRII
                     DEFVTGGIVEKKGNYYVFTDEGKKLVETIGK"
     gene            3691..4650
                     /locus_tag="VN377_06060"
     CDS             3691..4650
                     /locus_tag="VN377_06060"
                     /inference="COORDINATES: protein motif:HMM:NF037134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWR27982.1"
                     /translation="MFRNIPPILTAKQLLDQSIRRTKKILIHDRDKRYEIKKIVIART
                     EVFINDLTSRLESYVKNFPSIDKLPLFYQEIIDIKIDTDKLKKSLGAVDWARKTVMMV
                     YSTQSSSLTKSGNVEFLKQKQKEIYGRVSSIIKQVDKNLIFLSGAQKILRTFPDIEDV
                     PTVVIAGYPNVGKSSLIRQLSAAKPEIAQYPFTTKQIYVGHMVKTVRYEKKQYQIIDT
                     PGLLDRPLAERNNIEKQAIAALRHLADLIVFIFDPSGTSGYQMDEQTFMLENIKQLFN
                     DVPFIIVENKVDIKNTGSANRKISCSTGEGIEELRKEIFSHLN"
     gene            complement(4869..5204)
                     /locus_tag="VN377_06065"
     CDS             complement(4869..5204)
                     /locus_tag="VN377_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellum operon transcriptional activator EarA
                     family protein"
                     /protein_id="HWR27983.1"
                     /translation="MLEQTTIDRSIEIKRSVAIRALRRSNIRRKVTEYLCEISPNCSY
                     TSEIAYSIGATSSNVIGALRGIETRYREEESLIGLNMVEERSGGKNVRLYGVTDFGRE
                     IIQTMRTRR"
BASE COUNT         1334 a         1314 c         1195 g         1802 t
ORIGIN      
        1 tgttcgtcct acctgtacga cgatgtcttt gagtcgcgtg gttgcttgtt ttgcggtgaa
       61 tttgaacgcg atcgcccatc gtgggtgttt aatggttgaa cctaactccc tctgttgtgt
      121 gaacgagttt acctttagaa cgaccccgtc gatttcataa tcgagcgtct cgcgtttggt
      181 ctccagggtc ttgcaatagg agattgcatc ctctatggag gaggcttttt ctatcaatgg
      241 gttgacacgg aaccctgctg ttcgtaatgt ctcaagggct ttttcttggc tcttgaaatc
      301 tttatcagaa tgtgaaatgt agtacagata cgtgtctaat ggtcgtgatg cgacgatgcg
      361 aggatcaagt tgtcggagcg atcccgctgc cgcgttccgt gggttggcga acacctgttc
      421 tgcttttttc tcctgttctt tattgaattt tttaaatgat gatatcggga agtatacttc
      481 accgcgcacc tcagcgtttt gaagctctga tccttgcaac cggagtggga tgctccggat
      541 cgtcctgaga ttcgaggtaa tatcatcccc ttttatccca tcgccccggg tcgctccgcg
      601 tatgaagatt ccattttcgt agataagggc gatgctggcc ccgtcgatct ttggttcaca
      661 gatatattca acgtctgtaa tgtttttttt cacccgttcg tcgaattctc tgagttcatc
      721 ataggaatag gcgttatcca gtgaaagcat cggtatttta tgttccacgg ttgtgaatcc
      781 tttctgcggt tctcctccaa tccgttgtgt cggagaatct gaggtgataa gatcgggata
      841 ctgtgtttcc agggtttcga gtttttttaa cagcatgtcg tattcgtagt cagagatcac
      901 cgggttgttt tcaacatagt attgatagtt atggtagttg atgtcatcac gaagtttttg
      961 tatctgtttt ttcgcgtcgt ttttattcat tggttcacaa gccgattatt ttttctattt
     1021 cttgtaccgt tgaggtattc aggatgtctt ttttctcaag ccagcctctt cgggcggttg
     1081 cgattccaaa cctcatgaag tccagatgat tgatgctgtg tgaatcggtg ccaagtacga
     1141 aacgtacatc gtggtctttg gcttgtttta tgtggacgtc atcaaggtct agtctgtcgg
     1201 ggaacgagtt tatctcaagg cgggtatcgg tttctttcgc tgtgtcgatg atctgttcga
     1261 tatcgacctc gtaggggtct cgttttccaa tgagtctgcc tgtcggatgt gcaaggaaat
     1321 tgacctgttc gttgtccatg gctttaatga ttcgtttggt catggtttct ttgtccattt
     1381 taaacgcggt atgaatcgcg atgtacacga aatcaaactg ttgtaatgtt tttttgctat
     1441 agtccaatgt cccatctgtt tttatgtcgc attccgtccc acaaaagatc cgcagctcag
     1501 ggaatttctt ttcaactttt tttatctctt gtatcttttt ctgtactcgg tcctcagtaa
     1561 ggccatttgc gatttttagg gactgtgaat gatcggtgac cccgatgaat ctgtatccgc
     1621 gtttgtgtgc cgccgctgcg atggtttcga tggagtcact tccatcgctc cagatggaat
     1681 ggacgtggaa atcgccggcg atctcctcat atttgacaag tgtcggtaat gtgttgtttc
     1741 ttgctgcttc gaattctccc cggttctctc gtagctctgg ttcaatgtat ggcattccaa
     1801 tttttttgta gatttcttct tcggttcttc ctgagatgta tttttctgtt tttttatcaa
     1861 acaacccgta ttcgttcagc ttgtatcctt tcttgatcgc atatccacgt aatgtgacgt
     1921 tatggtcctt tgagccggtg aaatactgga gcgctgctcc gtagcttttc gcctcgacga
     1981 cgcgaaggtc cacctggaga ttgtcggcta agcgcacact tgatttggtc ggtccttttg
     2041 cgaggatttc tgcaacctca ggatactgga tgaaacagtc catgaccacc gaagggtcat
     2101 tggacgaagc aaggatgtca aggtccccga tggtttcttt catccgacgg aggcttcctg
     2161 cgatgtttat tttttcaatc tttttgcatt tctttagata tgtcaggtat cgattgccat
     2221 cctcatatgc gacgttcagc aggactctgc cgcttgtttt ttccagcagt tgaatcccgc
     2281 ggaggatgtt tcgttcggtg atctcgccaa atcctttcag cgttcgtagt tttccgttgc
     2341 tcgctgcttt ttggagatcc tggatactgg tgataccccg gtttttgtat aacgctgcca
     2401 cctttcttgg tccaagtcca gggatcccta agagatcgat taggcctgtc gggacttctt
     2461 ttttcaatcg ttcaagatat tcaagttttc cggtctggac gagttctatt atcttttttg
     2521 ctagtgcctc gccaacacca ggaattgatt ctaccgtacc ctgctgtatg attttttcga
     2581 tgtcttcatc gagagcttcg atggtttgtg cagcgatgcg gtacgccctg gtcttaaaga
     2641 acatctctcc tttcaaatcc aagaggtccg cgatcagata caaggtatca gcaacgaact
     2701 gattcttcat agcacctcat ctgtttttat tgaagatgga cgactatttc tacggcatca
     2761 ccatcatgca gcccaagcgt ttcacgtaga ttgtctgggg agatacattc aaggactgtt
     2821 gcgtaatggc ttcgtttcgg aagcacaatc gcccctttcc ttccattgat ggtagccttg
     2881 aaacagatga cactcccaaa cgttcgattc tctgcctgga actcatcgat ttcgatgcca
     2941 ccatagcttc tgagcaatcg tatcttattc ctctctatgt attcgatttc gacgttcaag
     3001 gtccccggat aggcgacaaa cccgagtcgt ttctcaaatt gatcagcgta gcctttgtgt
     3061 cccgtgtaat attttccctc acccatccct gagacaagct tccctttgaa cacgactttg
     3121 tttgtcagat caaagatctg tttgtacgag tgatattcat tttccagcgc ctcagccccc
     3181 ccaccagtaa tctggatcag ctgctttttc acccctagtt ctcgtgtgat catctcctgg
     3241 gtgtccagct cgagaaggta ccgtgacgcg gtctgctggc tcgtatgtaa ttgatcggca
     3301 agctcatgag acgatagctc gattttgtta tgaaccgccc ccagcaacgc aagctctttg
     3361 agggtctgca tcagatatgg tttcaagggg ttttttctgc tccccccttg tctattttcc
     3421 gatggtttca acaagttttt tcccttcatc cgtaaaaacg taatagttcc cttttttttc
     3481 gacgatgcct ccagtgacga attcatcgat aatgcgttgt gcgacacgcg gtatcatacg
     3541 attttttttc gcgatacgtt taatgtcgtt ttctccagcg acgagctcat cgaaaatcac
     3601 tcttctgaat ttatttgcaa gtatgaaccc ttcatccata ggcgtaccaa cagcttaatt
     3661 tagaggtatc acataaacct tttcaaactt atgtttagaa acatccctcc gattcttact
     3721 gcaaaacaac tccttgatca gtcgattcgc cggacaaaaa aaatactaat ccatgatcga
     3781 gataaacggt atgaaataaa aaaaatcgtc atcgcacgta ccgaagtctt cattaacgac
     3841 cttacctccc ggttagagtc gtatgtgaaa aactttccat ctatcgataa actaccattg
     3901 ttctatcagg agattattga tattaagatt gatacggata aattgaagaa atcgcttgga
     3961 gcagttgact gggctcggaa aacagtcatg atggtctatt caacccaaag cagctcgtta
     4021 acgaaatcag gtaacgtcga gttcctcaaa caaaaacaaa aggaaatcta tggtcgtgtc
     4081 tcatcgatta tcaaacaagt ggataaaaat ctcatctttc tctcaggtgc acagaaaatc
     4141 ttgagaacat tccccgacat tgaggatgtt ccgactgttg ttatcgctgg ttatcctaac
     4201 gtcggaaaat catccctcat caggcaacta tcggccgcga aaccagagat cgcccaatac
     4261 ccgtttacga caaaacagat ttatgtcggc catatggtaa aaacagtccg gtatgagaaa
     4321 aaacagtatc agatcattga cacaccaggc ttattagatc gtcctcttgc cgaacggaac
     4381 aatattgaga agcaggcgat cgcagcgttg cgccatctag ctgaccttat cgtgtttatc
     4441 tttgatccct caggaaccag cgggtaccag atggatgaac aaacattcat gctcgaaaac
     4501 atcaagcagt tgttcaatga tgtcccgttt atcatcgttg aaaacaaggt cgacataaaa
     4561 aacactggat ctgcgaatcg taagatatca tgttctactg gagaaggtat cgaggagctc
     4621 cgtaaagaaa ttttcagcca tcttaactaa tacaactttt tgtaggtgca tatccacgta
     4681 catgttcatc tgcgaaaatt gctcataaaa agaccatgtg atatcctttc taacgacaac
     4741 taggaaagga agatcttaga cttttatacc ctcttttccc ccctctattc ttccttccta
     4801 ttgtttgttt tttttaaaat ttttccttag aatttctgaa agactaaaaa aaaagaattt
     4861 attgatgttt atcgtcgtgt cctcatggtt tgaatgattt ctctaccaaa atcagtcacg
     4921 ccatagagcc gtacgttctt cccaccagat cgttcctcga ccatgttcag gccgatgagg
     4981 gattcttcct cacgataccg tgtttcgatt ccccgtaacg ctccgatgac gtttgatgac
     5041 gtcgcaccaa tgctatacgc aatttcagaa gtataactgc agttcgggct gatctcacag
     5101 aggtattctg tcactttccg tcgaatgtta ctccgccgaa gtgcccgtat cgcgaccgat
     5161 cgtttaatct cgatagaacg gtctatcgtt gtttgttcca gcatcccatc gtacccactc
     5221 aaaaaacgaa tgggacctct tttaataaag tattcgcttc cggatacctc gctgcgtatg
     5281 cacttgcaca actgcttgca taaccaccgc tatatcccga tatatatgca agcactatct
     5341 cattgcaaat taattgaatc tttttaaggt ggtattcaca gggagaaatg cgagtaattc
     5401 tgacatgtca gttagaccaa aaacccttac aaatgattca tggaacgaag atacaaccaa
     5461 aaatgataat gttaacctcc gtttgtacaa agctcatata gtataatgaa ataggggttc
     5521 cacccctata accccgttcc gccttcgttc ctcggcaagc cctatcgggc gttgccgtat
     5581 ctcactcggc ggtcatgcaa cggttggcaa tcgctgctgc ttttcatcaa taacgtacgc
     5641 attgt
//
LOCUS       DATMXJ010000228         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_77822, whole genome shotgun sequence.
ACCESSION   DATMXJ010000228 DATMXJ010000000
VERSION     DATMXJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77822"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VN377_06070"
     CDS             <1..713
                     /locus_tag="VN377_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27984.1"
                     /translation="KIIILTVLLIGSLMITPALASTTQAEYQTKLVRKNSETITVEFI
                     DVTGAIPVKKEITMTRAEWNTINNELQAVATTEKTMSGKFAAQLSVFQNHHLVSEQTS
                     IDALLAKFDKRTNHGLIRSLLERVPKSLPINNSIFSALSAITFTLENENGSTMVFGLN
                     TFINFIGFNIVSVHKGYAPNGIQTNGLISDSVPPGEYAGFIFGFFGYWFGEKISTGVY
                     SNLTVAGLGIITAWLPVP"
     gene            complement(758..1663)
                     /locus_tag="VN377_06075"
     CDS             complement(758..1663)
                     /locus_tag="VN377_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HWR27985.1"
                     /translation="MLSLKDFKKIRLEDKSIFDTHYQHHPPMHSGELFTTMISWAEYV
                     EYHYAKIHDSIIILSKDSNGTVLHAPSGTFDQDLFTQVMKLAIKEDCIFGFIKKTEKD
                     YLSNHYPTLKIIEDRDYFDYVYRASDLAELPGTKYGKIRNRLNKFTKNFSYTTEEITQ
                     NNMDEVNEFLKRWCLWKDCSSDELLENERKAIIYSMKHYFDFNLKGLALRIDGTIQAI
                     AVYEKMNPDTMVVHFEKGSPDYDGIYKAINMETAQRVRNLVPFIDREEDVGIPGLRQA
                     KLSYHPDHFIEVFYTEKESLRTTIV"
     gene            complement(1667..2092)
                     /locus_tag="VN377_06080"
     CDS             complement(1667..2092)
                     /locus_tag="VN377_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWR27986.1"
                     /translation="MEKKQIEIQLITTWPSDEIVTLYTAGGWWKDTYDTAGIPQLITR
                     SFAFAVAVDKNTGKSVGMGRVISDGVSDAYIQDLVVLPSYRGQSIGKKITQKLIDFCL
                     SKGIVWIGLIAEPGSSAFYTSLGFKTMEHHVPMLYTTEK"
     gene            complement(2120..2329)
                     /locus_tag="VN377_06085"
     CDS             complement(2120..2329)
                     /locus_tag="VN377_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWR27987.1"
                     /translation="MKKEFKEGNITITVDYDKCKGSGECVTACPVEIFELVDGKAVAK
                     KITECIECCACVNACPYTAIEHSSC"
BASE COUNT          643 a          500 c          517 g          749 t
ORIGIN      
        1 ataaaataat aattttaact gtgcttctga taggaagtct gatgataaca ccagcactag
       61 caagcacaac tcaggcagaa tatcaaacca aattagtaag aaaaaacagt gaaaccatca
      121 cagtggagtt catcgacgtt accggagcaa tccccgtaaa gaaagagatc accatgaccc
      181 gagcagaatg gaatacaatc aacaacgaac tccaagcggt agcaaccact gagaaaacga
      241 tgagcggtaa atttgcagcg cagctctcag tctttcagaa tcaccacctt gtttccgaac
      301 agacctccat tgatgctctt ctggcaaagt tcgacaaaag gacaaaccat ggattaatca
      361 ggtcattact ggaaagggta ccaaaaagtc tacctattaa taattccatc ttcagcgcac
      421 tcagtgcaat aacgtttaca ctggaaaatg aaaacggctc aacgatggtg tttggattga
      481 acacgttcat taattttatt ggtttcaata ttgtgagcgt gcataaggga tacgcaccga
      541 acgggatcca gacaaatggt ttgatctcag attcagtacc gcctggagaa tatgcagggt
      601 ttatctttgg tttcttcggg tattggtttg gagagaaaat atcgaccggg gtgtactcga
      661 acctgactgt tgcaggacta ggcattatca cagcctggtt accggtccca taaaccgttt
      721 cttaacgacg aaactaatcg attgaggact acaagggtca tactattgta gtccgtagac
      781 tttctttttc cgtatagaag acttcgatga aatgatcagg gtgataggat agtttcgcct
      841 gtcgtaaccc tgggattcct acgtcttcct cgcggtcaat aaaaggaacc agattgcgca
      901 ctctttgggc tgtctccatg ttgattgcct tataaatccc gtcataatct ggggaccctt
      961 tctcaaaatg gacaaccatg gtatctggat tcattttttc atacacagcg atagcctgaa
     1021 tcgtaccatc aatgcgtaac gcaagacctt tgaggttgaa atcaaagtaa tgcttcatcg
     1081 aatagatgat tgccttgcgc tcgttctcta gtaattcatc agagctgcag tctttccaaa
     1141 gacaccatcg tttcagaaac tcgtttacct cgtccatgtt gttctgggtg atttcctcgg
     1201 tggtgtatga aaagtttttc gtaaatttat tgagccgatt ccgtattttc ccgtattttg
     1261 tacctgggag ttctgcgaga tcagatgctc ggtacacata atcaaaataa tctctatcct
     1321 cgatgatctt taaggtagga tagtgattcg aaaggtagtc tttctctgtt tttttgataa
     1381 aaccaaagat gcagtcttcc tttatcgcaa gcttcatgac ctgcgtgaac aggtcttgat
     1441 cgaatgttcc agacggggcg tggagtaccg ttccgttgct atctttgctt aaaataatga
     1501 tgctgtcatg tatcttcgca tagtggtatt caacgtactc cgcccagcta atcatcgtcg
     1561 tgaacagttc accgctatgc atcgggggat ggtgttggta atgtgtgtcg aagatggatt
     1621 tatcctcgag acggattttt ttaaaatcct taagagatag catgtattat ttctccgttg
     1681 tatatagcat gggaacatgg tgttccattg ttttaaaacc aagggatgtg tagaatgcgc
     1741 tgctccctgg ttctgcgatc agtcctatcc atacaatccc ttttgagaga caaaaatcta
     1801 tcagtttttg tgttattttt ttcccgatgc tttgaccgcg gtacgatggg aggaccacga
     1861 ggtcttgtat atatgcatca gagacaccat cagatatgac acggcccatg cctactgatt
     1921 ttcctgtgtt tttatccact gcgactgcaa aggcaaaact cctggtgatt aattgaggaa
     1981 tccctgctgt atcataggtg tctttccacc agcctcctgc tgtgtataat gtgacgattt
     2041 cgtctgatgg ccaggtcgtt atcagttgta tttctatttg ttttttttcc ataaaaaaga
     2101 tccagtgggt ggaaggaatt tagcaggagc tatgttctat ggctgtatag gggcaggcgt
     2161 tgacgcaagc gcagcattcg atgcattctg tgatcttttt tgcgactgct tttccgtcga
     2221 ctagttcaaa aatttcgacg ggacatgcgg tgacgcattc acctgagcct ttgcatttat
     2281 cataatcaac tgtgatagtg atatttcctt ctttgaattc ttttttcata tgacaagtac
     2341 ctccttgatg ttacagtctg taacatctac tattatttta gatttcttgt ttatgttttt
     2401 ttggcttgt
//
LOCUS       DATMXJ010000229         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_4507, whole genome shotgun sequence.
ACCESSION   DATMXJ010000229 DATMXJ010000000
VERSION     DATMXJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4507"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VN377_06090"
     CDS             <1..545
                     /locus_tag="VN377_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR27988.1"
                     /translation="DRLIKCGSRMVGDKHLYLFEDQALLFEEQNTLYRHLEDGKITKN
                     ELNEQMKKSGRILILSNQEMSEQEAYELYKRRETVEKRFDAYKSTLSADRLYLQDDES
                     VFGHVFIAFLSLYAYSKLELLLKKADLNKKMTPIDLLFEFSKIYHIDFGIYGQVMEVP
                     KKIRDIEAKLGLKLFPTPKS"
     gene            524..1960
                     /locus_tag="VN377_06095"
     CDS             524..1960
                     /locus_tag="VN377_06095"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HWR27989.1"
                     /translation="MPHAKELRFKTVPVEHLLGKGISADDLTDDVLGRTLDAIHEYGA
                     AKLYNEITNSIMQQHSLGTELIHVDTTNFSVHGKYEGDAPDSTDSIKITYGHAKDGRK
                     DLKRFVLGLVANQFGIPLFTEAYDGNKSDKKTLIEMIKKTQKSINLDGQTIWIADSAM
                     YTAENIQELGTETLWITHAPETINEIKVLQNADIEMTPGKDPRYAFHVTELTHGGIPQ
                     RGVVVWSEEMQKRQEKTFDKMIQTKTDDAIKDLNKIANEPFVCVPDAENRLKKWRSEH
                     PLHRLKDEKISLKSTKIEKKRGRPKKGEPLETRYFIEAEVELDNDLVLRKRQKLGRFV
                     LASNKMDLDGETILANYKGQQKVEHGFRFLKDKCFHVAEIYLKKEERIESLSMIMVLT
                     LLIYAFGEWILRKKLRETGTTVSNQLKKPTQKPTMRWVFQIFMGVTHTVLIEGGSVVK
                     TIVHLTQEQVTILKLLGPECEKYYGLQG"
BASE COUNT          652 a          428 c          480 g          496 t
ORIGIN      
        1 atgatagact gatcaaatgt gggagcagga tggtcgggga caagcatctc tatctctttg
       61 aggaccaggc cctattgttt gaagagcaaa ataccctcta taggcacttg gaagatggaa
      121 agatcaccaa gaatgagctg aatgagcaga tgaagaagtc tggacgcatt ctcatcctat
      181 ctaaccaaga gatgtccgag caggaggcat acgagctcta caagaggagg gagaccgtcg
      241 aaaagaggtt tgatgcctac aaatcaacac tatctgccga caggctgtat ctgcaggatg
      301 atgagagcgt ttttgggcac gtcttcatag ccttcttatc cctttacgct tatagcaagc
      361 tggaattgct tttaaagaag gctgatctga ataaaaagat gacacccatt gacctgctct
      421 ttgagttcag taagatttat catatcgact tcggaatata tggacaagtt atggaagtcc
      481 ctaaaaaaat tagagatatt gaggcgaagc ttggattgaa gctattcccc acgccaaaga
      541 gttaaggttc aaaacagtcc ctgttgaaca tcttcttggt aaaggaattt cagcagatga
      601 cctcaccgat gacgtactcg gcagaacact tgatgcgatt cacgagtatg gtgccgcaaa
      661 gctctacaat gagatcacaa atagcatcat gcagcagcat tctctgggaa cagaacttat
      721 tcatgttgat accactaact tcagtgttca tggcaaatac gaaggcgatg ctccagacag
      781 taccgattcg atcaaaataa cctacgggca cgcaaaagat ggacgaaaag atctcaagcg
      841 ttttgtcctc ggccttgtcg cgaatcagtt tggcattcct ctttttaccg aagcttacga
      901 tggaaacaag tctgataaga agacccttat tgaaatgatc aaaaaaactc aaaaatcgat
      961 caatctcgac ggccaaacca tctggatagc tgatagtgcc atgtatactg ctgagaatat
     1021 ccaggagctg ggaacggaga cactatggat tactcatgcc cccgaaacaa taaacgaaat
     1081 caaggtattg caaaacgccg atatcgaaat gacgcccggc aaagatcctc gatatgcttt
     1141 tcatgtcaca gagctgactc atggaggtat tcctcaacgt ggcgtggttg tttggtctga
     1201 ggagatgcag aagaggcagg aaaagacttt cgataagatg attcaaacaa agacagacga
     1261 tgccataaaa gacttaaata aaattgctaa tgagcctttt gtctgcgttc ccgatgccga
     1321 gaatcgcctc aaaaaatgga gatctgagca cccccttcat cgactaaagg acgagaagat
     1381 aagtctcaaa tccacaaaaa ttgagaagaa gcgagggcgt cccaagaaag gcgaaccgct
     1441 ggaaactcgc tatttcattg aggcggaagt cgagcttgat aacgatttgg ttttaagaaa
     1501 acgtcaaaag ttaggtcgtt ttgtccttgc cagcaataaa atggatctcg atggggagac
     1561 aatccttgcc aattacaaag ggcaacagaa ggttgagcat ggattccgct tcttgaagga
     1621 taagtgcttt catgttgcgg aaatctactt gaagaaagag gagcggatag aatcactctc
     1681 catgatcatg gttctcactc tacttatcta tgcttttggc gaatggatcc taaggaaaaa
     1741 acttcgtgag actggaacga ctgtatccaa tcagctcaaa aaaccaacac aaaaaccaac
     1801 tatgagatgg gtgttccaaa tctttatggg agtcacccat acggtattga ttgaaggtgg
     1861 tagtgtggtc aaaacaatag ttcacctaac ccaagaacag gtaacgatac tcaagctact
     1921 tggaccggaa tgtgaaaaat actatggttt gcagggttaa tgcggaatgt gggctgtaag
     1981 ccttccagcc tctgggcttc agctggctgg gagatggctg gcagatagag cctaaactcc
     2041 gccctcttta ataacc
//
LOCUS       DATMXJ010000230         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_41268, whole genome shotgun sequence.
ACCESSION   DATMXJ010000230 DATMXJ010000000
VERSION     DATMXJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41268"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..442)
                     /locus_tag="VN377_06100"
     tRNA            complement(369..442)
                     /locus_tag="VN377_06100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Val,seq:gac)
     gene            complement(540..1412)
                     /locus_tag="VN377_06105"
     CDS             complement(540..1412)
                     /locus_tag="VN377_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWR27990.1"
                     /translation="MKRKMKFSVGIISMICIIMLITPACTAGLSSLTVKKKNTQPQQA
                     SASQYWALLFAVGTYEGNPDQDRPSMIEACDDLNGVLLDSPQYWQASNIHTQKGGQCY
                     LQNLIKELLWLRKNSKSEDYVFVYITTHGIQLQKNGSPWDLPPKDESDGADEALIMYN
                     GFSQWYGLIWDDLLNFFLSTIKCKALCLVVDSCYSGGFNDLPLPITKQKRYTAMSSQE
                     NEVSYGSDFSNLLITGLTNGLADTNIWLFGTSGNNDGIVSAQEAFRFAKFWLGYQTPT
                     EIDTFYSEFPLTYS"
     gene            1552..2580
                     /locus_tag="VN377_06110"
     CDS             1552..2580
                     /locus_tag="VN377_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWR27991.1"
                     /translation="MEAIILAGGFGTRLRPFTFTRAKSLLPIMNEPMIAHLIKTLPKK
                     VDTVILAVNYRREQIEEYFCNHDVGKTIIVNEEPEPLGTGGAVKFADKHIAGSFFVLN
                     ADIICSLNLTEMLRFHERKKAVATISLWPVENVSEFGVVDIRKDGTITKFVEKPKAED
                     APSDLINAGAYLLEPQVLDYIEPGKLVSMEKQIFPQIIDNTGRFFGYKFQGHWIDVGR
                     ISSYISVHKLLLEQQKKTFITGGQCSIQGVLRQSCVGNKVTVGTNTTLESSVLFDEVT
                     VGENVTLDHCVIGEHVSIGDGSVLRNVAVGDNERIQDGTSLDNVVVWNQPIPPGYPSK
                     QIGNVIGE"
     gene            2634..>3312
                     /locus_tag="VN377_06115"
     CDS             2634..>3312
                     /locus_tag="VN377_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27992.1"
                     /translation="MEQPVSKLIFRAYDIRGLYNKEISPDIFYKIGLAAGTYVKQTMR
                     GKQISVGGDIRQSSQCLAHAFIAGVTATGVHIHHTGTTSFGQTLFTGGELKADLIAFV
                     TASHLPPEWNGIKFYYGDGVGFPEENLMAIRDLVLQDSFELASWDHIGQVTPVDAKTG
                     YKDFFTPQFKFKKKLTVALDCGGASMTLSAPEIFTALGFHVIPVYCDLDPTFSKRPSD
                     PKPKNLTT"
BASE COUNT          938 a          715 c          738 g          921 t
ORIGIN      
        1 aaaagaaaaa agaaaaatcc ccctctcttt cattgttagg taagtaagtt taaaatttcg
       61 ttacaaatta tttgaacgac taagttagta ttttggaagt aaattaattt aaggctggta
      121 tatgcttatc tatagaatca aagaatgaat caaagaatga gtgttatgag aagatattta
      181 tgctttggaa tgcgtgtcta tgcgcatgag caagaaacca ttgaacttat tgattgaggc
      241 tgatttggtc gagaaagcac gagacaaggg tattgagata agcaagttct tagagaacaa
      301 actccaggag tattttcaat tccttgagat ggtcagtaag ccacaaggtc cgtatcaaga
      361 gaatggagtg ggctcgttga gattcgaact caagacctcc tccgtgtcag ggagacgtca
      421 taaccaacta gaccacgagc ccaatagcaa aaacactatc acttcgtgta gcgtaaccag
      481 aatttctatt actttttaaa tgtttcagta accccttatc ctgggtgatt cagaatttat
      541 tatgaatagg tgagaggaaa ctctgaatag aacgtatcaa tttctgttgg ggtttgatat
      601 cctaaccaga atttagcaaa tcgaaatgct tcctgggctg aaacgatgcc gtcgttgttt
      661 ccactagttc cgaataacca gatgttggta tctgctaatc cattcgttaa accggtaatt
      721 agtaaattgg aaaaatctga tccatacgat acctcatttt cctgactgga catcgccgta
      781 tacctctttt gcttcgtgat gggtaatggc aaatcattga atccaccgct atagcaacta
      841 tcaacaacaa gacagagtgc cttacatttt atcgtactga ggaagaagtt taacaggtca
      901 tcccagatga ggccatacca ttgactaaat ccattgtaca taataagagc ctcgtctgcc
      961 ccatcagact cgtcttttgg tggaagatcc cagggtgaac cgtttttttg caattgtatt
     1021 ccatgagtgg tgatgtagac aaaaacataa tcctcacttt tagagttttt tctcagccag
     1081 agcaattcct taatcagatt ctgaaggtaa cattgtcccc ctttctgggt atggatatta
     1141 ctggcttgcc agtattgtgg ggaatccaga agtacaccat tcaaatcgtc acaggcttct
     1201 atcatcgatg gacggtcttg gtccgggttc ccttcatagg ttcctacggc aaataataat
     1261 gcccaatact gtgatgctga tgcttgttga ggctgtgtgt ttttcttttt taccgtaagc
     1321 gagcttaatc cagcagtaca tgctggtgta atcaacatta tgatacatat catcgatatg
     1381 atgcctacgc tgaatttcat ttttcttttc atagtatccc ctgagatatc tactaattat
     1441 aataatttta tcatataaat tcttttcgtt tcttttcgtc acgtgggaaa aagaaacgat
     1501 ttaatacgta gcatctattt ctgggtctaa taatatcttt aagggattca tatggaggca
     1561 atcatccttg caggaggctt tggcaccaga cttcgaccat ttacctttac ccgggcaaag
     1621 tcactattac ctattatgaa cgagccgatg attgctcatt tgattaagac acttcccaaa
     1681 aaagtcgata ccgtcattct cgcggtgaac taccgccgag aacaaatcga ggagtatttc
     1741 tgcaaccatg atgttgggaa aaccatcatt gtcaacgaag agccagaacc gctggggacc
     1801 ggtggtgctg tcaaattcgc tgataagcac atcgccggat ctttttttgt gttgaatgct
     1861 gatatcatct gttccttaaa cctcacagag atgctcaggt ttcatgaacg gaaaaaagcg
     1921 gtcgcgacga tctcattatg gcctgttgag aatgtctcag agtttggcgt cgtcgacatc
     1981 agaaaagatg ggactattac gaagtttgtc gaaaaaccaa aggcggagga tgctccctcc
     2041 gatctgatta atgcaggggc gtacctgctc gaaccacagg tgctcgatta cattgaacca
     2101 ggcaaactgg tttctatgga gaagcagatc ttcccgcaga ttattgataa caccgggcgt
     2161 tttttcgggt ataagttcca ggggcattgg attgatgtcg gcaggatcag cagctacatc
     2221 agcgttcata aacttttgtt ggagcagcag aagaaaacat tcatcaccgg aggacagtgc
     2281 tccatccaag gtgttctgcg acagtcctgt gtcggcaaca aggtgacggt cggtacaaat
     2341 acgacccttg aatccagtgt gttgtttgac gaagtcacag ttggagagaa cgtcacttta
     2401 gatcattgcg tgattggtga gcacgtttcc atcggtgatg gctctgtgct taggaacgtc
     2461 gcggttggtg acaatgaacg aatccaggat ggcacatcgc tggataatgt agtggtgtgg
     2521 aaccagccaa tcccaccagg ataccccagc aaacagattg ggaacgtgat cggggagtaa
     2581 accaaggtct tcagcaaaca tatttttata acaggcaact catgtgctct gtcatggaac
     2641 aacctgtttc aaaactcatt ttccgggcgt acgatatcag aggattgtac aacaaagaaa
     2701 tctcccctga tatcttttac aaaatcgggc ttgccgctgg aacgtacgtg aaacaaacca
     2761 tgcggggaaa acagatcagt gtcggtggtg atatccgcca gtctagccaa tgccttgccc
     2821 atgcgttcat cgcaggagtc actgccacag gggtccatat ccatcatacg gggacgacgt
     2881 catttggtca gacacttttt accgggggtg aactgaaggc tgacctgatt gcgtttgtca
     2941 ccgccagtca cttgccaccg gaatggaacg gaatcaagtt ctactacggt gatggtgtag
     3001 gattccctga agaaaacctc atggcgatca gagaccttgt tctccaggat tcttttgaat
     3061 tggcctcatg ggaccatatc ggccaggtga cccccgtcga cgctaagaca ggttataaag
     3121 acttcttcac tccccagttc aaattcaaaa aaaaactcac ggtcgccctt gactgtggcg
     3181 gcgcaagtat gacgctatcc gcaccagaga tattcaccgc gttaggattt catgtcatcc
     3241 cggtgtactg tgacctggat cctacgttct cgaaacgtcc atcagaccca aaaccaaaga
     3301 atctcacgac ac
//
LOCUS       DATMXJ010000231         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_56985, whole genome shotgun sequence.
ACCESSION   DATMXJ010000231 DATMXJ010000000
VERSION     DATMXJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56985"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2993)
                     /locus_tag="VN377_06120"
     CDS             complement(<1..2993)
                     /locus_tag="VN377_06120"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWR27993.1"
                     /translation="MIASATILITPEYYQVKAASGESSVGDENGVGINTSYICYIADN
                     LSWIGRTHDRGRAFGTDGEYHASILIENQMNQIGLYEVNTDEIKGTAQHPDLNATLKV
                     VSKGILINGTQSITDCFITPRWNTTFISLLDPDLAENASHLTYNYSYTDPLPIYQKPN
                     FSAFDELFNDLEFLDYLAENLSLRKLDTKLSWIIFFMNQLERIFNFTYENINVSDPDT
                     LPSWYNGNITEPKCGPYLLIEEDPSYNPNATIPSVLLDEFEPLSAPRAIYHALKLIIE
                     MSFWNLTHHNCMGLIRYDFNNDTSDMTNGLLNARPILYINGSVGRPIYENATLLTPSN
                     REISFWINQSYNPSIKSYNVFGQINGTNPNKTVLIDCLYDCWWNQGTADSAIGMGTVL
                     ALAKYFKDHNITPKYTLKFVAFGGEEYGFLGAQHYNDTHTNENITTVIDLNQIGFSQT
                     GSLPQTMFVHTNNESLKSLLRCITNDTHYKERTGTPFLHIGYTYYGAPSDDQPFAIAC
                     LPHKGNRSLNTICFLKDMNWTLHHRDGHNHDLGDVMTYYNETDINVTSEMIWNITKYF
                     TVDPNCWFSNISFTTFDSPNDGDTLNDSIRANFTIHTIIPQDKVRVVLDLGSKVGEFN
                     TLQLGADTIDYIVSSGGVEQSYVFTIPDNCTEGNYSLSFKLYNSTGRINEIINLNGGG
                     TYYNDTSDTSNWYHLYHPLGYPKVGNSYESVNDRICGSVFTATQNGRADNITAYINQN
                     FFEPTHYKCMIYRTNDSLLIGTTTENWISRDGLALSPWWAVFNFTDSKPVLVKGTEYM
                     ITCWGDNALSGIYYNESFFASTGAFDNETYGNPPNPGNFTNESRYYSIYCSYTPDITA
                     LQITNVTATPSIVGFGYNVTISANVTEDSSNVSLVKVQVNYPGGGSSNNTMTHTTGDT
                     YQYVFTDTWLVGQHNYSIYAIDDDNNSRTVTGFSFNVSADATITIATLEYSYTHDEFI
                     NITDPPSPLNNYMLVDRGIDWDKYY"
     gene            complement(2935..3207)
                     /locus_tag="VN377_06125"
     CDS             complement(2935..3207)
                     /locus_tag="VN377_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27994.1"
                     /translation="MNTALSQKSTCSTIGLNTEEKSFPEAVNTFETKPKNTFVNLSLI
                     NINKNVLTLSRGKKDEEKIVGNIGLYADDCLGDDIDYTRILSGKSC"
     gene            3405..>4953
                     /locus_tag="VN377_06130"
     CDS             3405..>4953
                     /locus_tag="VN377_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR27995.1"
                     /translation="MPMTSGQNTDSTNSLEIKTTSIKTTQTETNGGLQNSSWPMFHHD
                     TCHTGRSPYGPAGNWPSIKWKFLMEGWTVSSPAIDENGIVYIGAEDFDKSFFAIYPNG
                     SEKWHFASGGWIDASPAVGENGVIYSGSMNGDLYAFYPNGTIKWSIHLGSGWVFSSPV
                     IDNDGTIFAASLGSSRLCAVTPNGVIKWYFNATDWIYSSPAIDGNGIIYIGSHDHYLY
                     AVYPNGTLKWKYQTNGEVKSSPSIGDDGTIYVSSWDSYLYAITPNGTLKWKFSTGDTA
                     ETSPAIASDGTIYVGSYNGLLFSVSPAGNENWRFQTGNEIYSSPAIDTYGIIYCGSDD
                     GYLYALNHDGTLRWKFDAGDHMESSPVIGNDGTIYIAAQFFPSENQSFYTYLYALHLI
                     NNSPPSTPTIDGPSSGKAKQNYDYTIVSTDSEGNNISYYISWGDGTNTGWIGPYTSAQ
                     EQAVNHIWNKQGTYTIQVKAKDNYSAESDWETLSVTMPYELPHFRLFEWLFERFPNAF
                     SILRYILD"
BASE COUNT         1413 a          913 c         1055 g         1572 t
ORIGIN      
        1 tcatagtatt tatcccaatc gataccacgg tcaactagca tgtaattatt gagtggtgat
       61 ggtgggtcgg tgatgttgat gaactcgtcg tgtgtgtaac tatattccag ggtggcgatg
      121 gtgatggtcg cgtccgcgga gacattaaaa ctaaatcctg tgacggtacg actgttgtta
      181 tcatcatcaa ttgcatagat actgtagttg tgttgtccga caagccaggt gtctgtgaac
      241 acgtactggt aggtgtctcc ggtcgtgtgg gtcatggtgt tattacttga accgccaccg
      301 ggataattaa cctgaacttt gacaaggctc acgttgctac tatcctccgt gacatttgca
      361 gagatcgtga cgttgtaacc aaaaccaaca atagaaggtg ttgcagtgac gttggtgatc
      421 tgtagcgctg tgatgtcggg ggtgtagcta cagtagatag aataataccg ggattcattg
      481 gtgaaatttc ctgggtttgg tggattccca taggtttcat tatcaaatgc tcccgttgat
      541 gcaaagaaag attcgttata atatatccct gaaagagcgt tatcgcccca gcatgttatc
      601 atatattcag ttccttttac gagaacaggt ttactatcgg tgaagttgaa tactgcccac
      661 cagggagata atgccagtcc atcccgagag atccagtttt cagttgttgt accgataagc
      721 aacgaatcat ttgttcgata gatcatacat ttataatggg taggttcaaa gaaattttga
      781 ttgatataag cggtgatatt gtcagcccta ccattttgag ttgcagtgaa aacagagccg
      841 cagattctat catttacgct ttcataggaa tttccaactt tcggatatcc caacggatgg
      901 tatagatgat accaatttga agtgtcgctg gtatcattat aatacgtgcc gccaccattc
      961 agattaatga tctcattaat tcgtccggtg gaattataca gcttgaaact caacgaataa
     1021 ttcccttcag tacaattatc tgggatggtg aacacatatg attgttcaac gccaccagaa
     1081 gacacaatat aatcaatagt atctgcacca agttgtaatg tattaaattc accgaccttg
     1141 gagccaagat ctaagacaac tcgtacttta tcttggggta ttatggtgtg tatcgtgaag
     1201 ttcgctcgta ttgagtcgtt gagggtgtcg ccatcgtttg gtgaatcaaa tgtggtaaat
     1261 gagatgttac tgaaccaaca gttagggtct accgtgaaat attttgtgat attccatatc
     1321 atctcgcttg ttacattgat atcagtctca ttatagtaag tcattacatc ccctaaatca
     1381 tgattatgac catctctgtg gtgcagtgtc cagttcatat cttttaagaa acaaatggtg
     1441 tttaacgagc gattgccttt atgtggtaag caggcaattg cgaacggttg gtcgtcgctt
     1501 ggtgctccat aataggtata cccgatgtga agaaaaggtg tcccggtgcg ttctttataa
     1561 tgggtgtcat tggtgataca tcgaaggagg gacttaagag attcattatt tgtatgaaca
     1621 aacattgtct gaggaagtga tccggtctgg ctgaatccta tttgattgag atctatcacc
     1681 gtagtgatgt tttcattcgt atgggtatca ttgtaatgtt gtgcaccgag aaagccatat
     1741 tcctcaccgc cgaaggcaac gaattttagt gtatactttg gggtgatgtt gtggtctttg
     1801 aagtattttg caagggctag tactgtcccc atgccaatcg cggaatccgc tgttccttgg
     1861 ttccaccagc aatcatacaa gcagtcaata agaactgttt tatttggatt tgttccgttg
     1921 atttgaccga aaacattata actttttatg gagggattgt aactttggtt aatccagaaa
     1981 ctaatttctc tatttgaagg agttaggaga gtcgcgtttt cgtaaattgg tctaccaaca
     2041 cttccgttaa tgtataggat aggtcgagca ttgagcagac catttgtcat atcagaggta
     2101 tcgttgttga aatcatagcg aattaatccc atacaattat gatgtgtaag gttccagaaa
     2161 ctcatctcta tgatgagttt tagagcatga tatattgccc taggagcaga aagtggttca
     2221 aattcgtcta acaatactga ggggatagtt gcatttgggt tatagcttgg atcttcttca
     2281 attagaagat aagggccaca tttaggttct gttatatttc cgttgtacca cgatggtagt
     2341 gtatctggat cagatacatt gatattttca taggtaaaat taaaaattcg ttctagttga
     2401 ttcataaaaa atattatcca tgatagttta gtatcaagtt ttctaagaga taggttttca
     2461 gcaagataat ccaagaactc taaatcattg aatagttcgt caaaagcgga aaaattaggt
     2521 ttctggtaaa taggtaaagg gtctgtatac gaatagttat atgttaagtg acttgcattt
     2581 tctgccaaat caggatctaa aagagatatg aacgtcgtgt tccatcttgg tgtaatgaag
     2641 caatctgtta tcgactgcgt cccgttaatt agtattccct ttgatactac tttcaatgtt
     2701 gcattgaggt caggatgttg agcagtacct tttatttcat ctgtattgac ttcatatagt
     2761 ccgatttgat tcatctgatt ttctataagg atactagcat gatattctcc atcagttcca
     2821 aatgctctac ctctatcatg agttctacca atccagctta ggttatcagc aatatagcaa
     2881 atatatgacg tgttgattcc aacgccattt tcatcaccta cagaactttc tccgctagca
     2941 gcttttacct gataatattc tggtgtaatc aatatcgtcg ccgaggcaat catcagcgta
     3001 caaaccaata ttaccaacaa ttttttcttc atctttttta cctctgctta atgtaaggac
     3061 gtttttgttg atattgatga ggctgaggtt aacgaaagta ttcttaggct ttgtctcaaa
     3121 agtattcacc gcctccggga aggatttttc ttcggtatta agacctatag tagagcaggt
     3181 tgatttttga gacaacgccg tattcatatt tttactcgaa aatgttctcc atatgatatt
     3241 ttccggttat ttagaattaa ttagatgaaa ttaatctaag tgctaaatat ttatatatga
     3301 tatcaccctt taatattaat agaaaaatta caggggaatt gagtttgagc gaaaggaatt
     3361 tgttaaaaaa ttgctttgtg ctgatgtgca ttatttccat gataatgccc atgacatcag
     3421 gacagaatac tgattctaca aattctttag aaatcaagac aacatccata aaaacgactc
     3481 aaacagaaac gaacggaggt ttgcaaaatt cttcctggcc gatgttccat catgatacct
     3541 gtcacacagg aagaagccca tatggccccg ctggtaattg gccttcgata aaatggaaat
     3601 tcttgatgga aggttggact gtttcttctc cggctattga tgaaaatggt atcgtctata
     3661 tcggcgctga ggattttgat aaaagttttt ttgctattta tccaaatggt tctgaaaaat
     3721 ggcattttgc ttctggtggg tggatagatg cttcaccggc tgtgggagag aatggagtta
     3781 tttactcagg atcgatgaat ggcgatcttt atgcgttcta tccaaatgga acgataaaat
     3841 ggagtataca tctaggatct ggttgggttt tttcatcacc cgtcattgat aatgatggaa
     3901 caattttcgc tgcatctttg ggcagtagca ggttgtgtgc tgtcactcca aatggggtga
     3961 ttaaatggta tttcaatgcg actgattgga tttattcttc gccagctatt gacggtaatg
     4021 ggattatcta cattggttcg catgaccatt atctatatgc tgtatatcca aatggaacat
     4081 tgaaatggaa atatcaaaca aacggtgaag tcaaatcatc tccgtccatc ggcgatgacg
     4141 gtacgatcta tgtttcttca tgggatagtt atctgtatgc gataacccca aatggaacgc
     4201 tgaaatggaa atttagcact ggtgacaccg ctgaaacatc cccggcaatc gcatcagacg
     4261 gaacaattta tgtaggatct tataacgggt tgctctttag cgtcagtccc gccggaaacg
     4321 aaaattggcg ttttcagaca ggtaatgaaa tatactcctc tccagccatt gatacatatg
     4381 gaatcattta ttgcggttcc gatgatggtt atctctatgc tctcaatcat gacggaacgc
     4441 tgcgatggaa atttgatgcg ggggatcata tggaatcgtc acctgtaatt gggaatgatg
     4501 gaacgatcta tatcgcagca caattttttc catctgagaa tcaaagcttc tatacctatc
     4561 tctatgcgct ccaccttata aataactcac ctccatctac accaacaatt gatggacctt
     4621 cctccggaaa agcaaaacaa aactacgatt ataccatcgt ttcgactgat tctgaaggaa
     4681 acaacatttc ttactatatt agttggggcg acggtacaaa caccgggtgg atcggaccat
     4741 atacttcagc tcaagaacaa gccgtgaatc acatctggaa caaacagggg acctacacca
     4801 ttcaagtcaa ggcaaaggac aattacagcg cagagagcga ttgggaaaca ctttcggtga
     4861 caatgccgta tgagttgccg cattttcgac tctttgaatg gttatttgaa cgatttccca
     4921 atgcattctc aattctgcga tatattctag att
//
LOCUS       DATMXJ010000232         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_9274, whole genome shotgun sequence.
ACCESSION   DATMXJ010000232 DATMXJ010000000
VERSION     DATMXJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9274"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VN377_06135"
     CDS             <1..480
                     /locus_tag="VN377_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWR27996.1"
                     /translation="GYTLARRVVEEAEFSYTDEFPDGLYPRKTIGEIMLTPTRIYVKE
                     ILELFKKIDVHGLAHITGSGLWNIPRLKEKVKYLIENPLGPQPIFTFLQKHGNIDDYE
                     MYQIFNMGMGFTVIVENEDAEETTKILQNYSDATVKRVGTVQKGTGVEVPHLKLRYH"
     gene            506..1441
                     /locus_tag="VN377_06140"
     CDS             506..1441
                     /locus_tag="VN377_06140"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HWR27997.1"
                     /translation="MKIGTVEIPNRFVLAPLAGISCTAFRMLCKENGAGLIYTQMIDA
                     SLISGKNHHDVKEFLNIQEMERPVAVQLIGSEKNVLVKAVQAVEQFADIIDLNVGCIE
                     EDFLQKRCGASLLKDLPKLEELLRAMVDATEKPVSAKIRIGWDNQHINGVAVAQLIEN
                     AGASALCVHGRTADQGYAGKVNWTIMRQIKQKLKIPVIANGDVTSYNDGLSLLQKTGC
                     DLVMIGRQAQHCPWVFHEKPVDIKQQILHFIELYEQYENRQEPVEVADHVFWMLRDFK
                     TSEDTKKVHLIQTIPRITRYVNRLSQDGEHTTDQR"
     gene            complement(1383..2150)
                     /locus_tag="VN377_06145"
     CDS             complement(1383..2150)
                     /locus_tag="VN377_06145"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HWR27998.1"
                     /translation="MKKTSSERFWEIDALRGIAIILMILYHLVFDLNYFGLFTTDLDS
                     LPVLLFLYPVSTTFLLLVGISLTISYSRAQQKLTERQLQVKFLKRGTSVFGLGLFITL
                     VTWVYPHDGYIVFGVLHCIGLSILLAYPLIRFRNFPLILGVICIILGIILRVTVIVDF
                     PWLLWLGFVPSGFYTLDYFPLLPWFGVVLIGIFLGNSYYQDNKRKVSFNDHSQFILSR
                     GLCFLGRHSLIIYLLHQLLIVGLLYVLHLVINGSRIV"
     gene            complement(2210..2319)
                     /locus_tag="VN377_06150"
     tRNA            complement(join(2210..2280,2316..2319))
                     /locus_tag="VN377_06150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2247..2249),aa:Met,seq:cat)
     gene            2468..2683
                     /locus_tag="VN377_06155"
     CDS             2468..2683
                     /locus_tag="VN377_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR27999.1"
                     /translation="MEKKLLETRLVRRHSQFPTVCIYCNKQIPPDNLHYVEEGITDHI
                     HSLIARKYCESCYTKYGEQILLHEKTL"
BASE COUNT          938 a          614 c          646 g          654 t
ORIGIN      
        1 ggttacactc ttgctagacg agtggtcgag gaagcggagt tctcgtacac cgatgaattt
       61 cctgatggat tgtacccgag gaaaaccatc ggggagataa tgcttacgcc aaccaggatt
      121 tatgtaaaag agatccttga attgttcaag aaaatcgatg ttcatgggct ggcgcatatc
      181 accggcagcg gattatggaa catccctcgc ttaaaagaaa aagtaaaata tttgattgaa
      241 aaccccttgg ggccacagcc gattttcacg ttcctgcaaa agcatgggaa catcgatgat
      301 tatgagatgt atcagatctt caatatgggg atgggattta ccgtcatcgt ggaaaatgag
      361 gacgcagagg agacgaccaa aatccttcag aattactctg atgctacggt gaaacgtgtg
      421 ggtactgttc aaaaaggaac aggggtcgag gtccctcatc tcaagttacg gtaccattaa
      481 caataatcct tctttgtggg gtcccatgaa aatcggaact gtcgagatac cgaatcgttt
      541 tgtgcttgcg cccctagctg gaattagttg taccgcattc cgaatgttat gtaaggaaaa
      601 tggtgcaggc ttgatctaca cccagatgat cgacgccagt ctcatcagtg gaaaaaacca
      661 ccatgatgtc aaagagttcc tgaacatcca ggagatggaa cgacccgtgg ccgttcagtt
      721 gatcggcagt gaaaaaaacg tccttgtaaa agctgttcaa gcagtcgaac agttcgctga
      781 catcattgat cttaacgtcg gatgcatcga agaagatttt ctgcagaaga gatgtggtgc
      841 ttcgcttctg aaagatctcc caaaactcga agaactactc agggcgatgg tggatgcgac
      901 agaaaaaccg gtgagcgcga agatccgcat cggctgggac aaccagcaca tcaatggtgt
      961 cgctgtcgca cagctaattg agaacgcagg tgcatccgca ctctgcgttc atggtcgtac
     1021 cgcagatcaa gggtatgctg ggaaggtcaa ctggacgatt atgagacaga tcaaacaaaa
     1081 actcaagatt ccggtgattg caaatggtga tgttacatca tacaacgatg ggctgtcgtt
     1141 gttacaaaaa accggttgcg atttagtgat gatcggcaga caggcacagc actgcccatg
     1201 ggtgttccat gaaaaaccag tcgatatcaa acagcagatt ctccatttca tcgaattata
     1261 tgagcagtac gagaacagac aagaacctgt cgaggtcgca gatcatgtgt tctggatgct
     1321 gagagacttt aaaacatctg aagacacaaa aaaagtgcat ttgattcaaa caatcccgcg
     1381 gatcacacga tacgtgaacc gtttatcaca agatggagaa catacaacag accaacgatg
     1441 agaagctgat ggagcagata gatgatcagc gagtgtctcc ctaaaaaaca caatcctcga
     1501 ctcagaatga actgtgaatg atcattaaat gatacttttc ttttattgtc ttgatagtaa
     1561 ctattcccaa gaaaaatccc gattaacaca acaccaaacc aggggagtaa aggaaaataa
     1621 tcaagggtgt aaaaaccaga agggacaaaa cccagccaca gcaaccaagg aaaatcaacg
     1681 attactgtga ctcggagaat tataccaagg atgatacaaa taactccaag aatgagcggg
     1741 aagttacgaa accgaatcaa aggatacgcc aagagaattg aaagaccaat gcaatgcagg
     1801 acaccgaaca caatgtaccc atcatgagga tacacccaag tgacaagtgt gataaacaga
     1861 cctaatccaa aaacactggt ccctcgtttc agaaacttaa cttgcaattg tcgttctgtt
     1921 aatttttgtt gggcgcggga atagcttatc gtgagagaaa taccaaccag cagcagaaac
     1981 gtggtgctaa caggatacaa aaacaacagt acaggaagag aatccaaatc tgtcgtaaat
     2041 aacccgaaat agttcagatc aaaaacaaga tgatacagaa tcatcaggat gattgcaata
     2101 ccgcggagag catcgatttc ccagaaccgt tctgaagatg tctttttcac aaaatgaccg
     2161 gaaaagaaat catcttataa acctgatgtt tgaaagaaaa aaaagaaaat agcggggaat
     2221 ggatttgaac cattggtcta cgggttatgg gcccgtcggg atttcctgac taccccaccc
     2281 ttataggcac caccccaaaa gatgtgatgg ttgctcgcta tgggaggcag tcttccgccg
     2341 ccttctgctg tgtctttcgg gaataccgtt gcctatactt aaacctagca cccgagtaca
     2401 tcctatagac aagagaaaac ggaaggtttt aatacctcaa caaaggaata taatgggagg
     2461 aaagatgatg gagaaaaaat tacttgaaac acgcttggtg agacgacatt cgcaatttcc
     2521 caccgtgtgt atttactgca acaagcaaat ccccccggac aatcttcatt atgtcgaaga
     2581 ggggatcacc gaccatatcc attcgctcat cgcacggaaa tattgtgagt cgtgttacac
     2641 caaatacggg gaacaaatcc tactgcatga aaaaaccctg tgaccccaca gagcgatagt
     2701 gcttttccat tctgtaatgt caatagacat cgctaacatt cgtaataaaa tgatgaaggt
     2761 attccctcca ccccgaggga atatcgcact gtgttgaacc attctatata ttcatccaga
     2821 agaagggatg cattttcaaa caccttgtcc ag
//
LOCUS       DATMXJ010000233         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_46644, whole genome shotgun sequence.
ACCESSION   DATMXJ010000233 DATMXJ010000000
VERSION     DATMXJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46644"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VN377_06160"
     CDS             complement(<1..385)
                     /locus_tag="VN377_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWR28000.1"
                     /translation="MVLAQESVVNRTETIALVTMASLVGLGIFQIILGESISHSVSLI
                     GNGFDCIGDGFVSGIVWAGLRFFKKPADQRFHFGYYKLENLASIAATIVMFLLAGYII
                     LRSYNQLINPEEVQLPFIGAIVAFIA"
     gene            591..830
                     /locus_tag="VN377_06165"
     CDS             591..830
                     /locus_tag="VN377_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28001.1"
                     /translation="MVKINITIEGEDVSEIINELLHMGIELLKTNRKEIREVLKILAD
                     ESLEILNEKKTLEKGAELYRKINSEINWTYKRDKE"
     gene            complement(846..1934)
                     /locus_tag="VN377_06170"
     CDS             complement(846..1934)
                     /locus_tag="VN377_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWR28002.1"
                     /translation="MQRVKNVFRNSSQAVDCIILKNATIPYVDDNFFYVTNLDQGLFE
                     GSCAVLHSDGTVDLLVSELEAESARKSTARLAIYKTKEEFSTELKHLITPFSTIGVNA
                     SGLSYLDFNKFTGLVPNTKFVDVSDGFFKSRLIKDEQEIQRIKKACEIADETMVTIPE
                     IVAEGMSEDELAAEINYSLQSLGAERPAFDTISSFGKNTAEPHYGHGNVKLENGSVVL
                     CDFGACYKKYNSDITRTFVFGKATGKHKEMYETVLQAQQIAFQAIKPGVKACDVHHAV
                     HSFIDSTKFKGTFIHSTGHGLGLAVHDGIGFAPDNAMELKENMVLTVEPGVYLPGFGG
                     VRIEDDVVVKKDGLELLTKSSREFIELG"
     gene            complement(2018..2404)
                     /locus_tag="VN377_06175"
     CDS             complement(2018..2404)
                     /locus_tag="VN377_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HWR28003.1"
                     /translation="MKRKKSMVLDDNENKAVQLFSELGMPKNLAKTLLYISQVDECRS
                     ADVEQGADLRQPEVSVAMQELRRRGWAKKRDEKKKGKGRPVHLYKLTADLPSILKSFE
                     KEKMQEVENIRTDLDQLHNLITSFEK"
     gene            complement(2487..2933)
                     /locus_tag="VN377_06180"
     CDS             complement(2487..2933)
                     /locus_tag="VN377_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HWR28004.1"
                     /translation="MEVRPSYDEYFMEMAYVVAKRSTCLRRKVGAILVKEKHILSTGY
                     NGAPKGLRHCSETGCLREDQNIPSGERHELCRGLHAEQNAVIQAAVFGVSIKGSVLYC
                     TNTPCVVCVKMLINAGVTEIIYAGEYPDELAKQMMSESNLKIKRFK"
     gene            complement(2976..>3653)
                     /locus_tag="VN377_06185"
     CDS             complement(2976..>3653)
                     /locus_tag="VN377_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HWR28005.1"
                     /translation="VYQNLKKLIRYLITNNIGKVVGILVTPLFGYPVPLMPLQLLWSN
                     VIMESFPSIGVSTDSADKNIMKRNPSKMSEPIISRKHRIIVIVDGIIFGLSIAAGYIL
                     CFEYLKAQGIGLEQAGLMAGTVAFAITLLSPQIYVFILREGTFIEKFKRKNILLKSFF
                     VFTFLMILAIIFVPGLNTLFTTAPVFDPVIWGIILGFSLLTTVFRAVLGDHLFFKRNG
                     ESQSSAE"
BASE COUNT         1114 a          766 c          727 g         1046 t
ORIGIN      
        1 cagcgataaa tgccactatc gcgccaataa acggaagctg aacctcttcg gggttaatca
       61 gctgattata ggatcgaagg ataatgtaac ccgctaacaa aaacatgaca attgtcgcag
      121 caatggaggc gagattttcc agcttataat acccgaaatg gaaccgctga tccgctggtt
      181 ttttgaagaa tcgaaggcca gcccagacta tccctgagac gaacccatct ccgatacaat
      241 cgaaaccatt tccaataagc gaaacgctat gagaaataga ttcccccaga ataatctgaa
      301 aaatgccaag accgaccaac gatgccattg tcacgagagc aatagtttca gtacgattga
      361 caacagattc ttgggcgaga accattacac cagagaaaga acaacctatt attaagaatt
      421 attatttaga gaacaaaaga agaaggacat gtgatgattt ttttatttgt agaaaaatgg
      481 cataaaaaaa tctagttatg aaaaagtatt taagtggtgg ttttcatggc aggatatcca
      541 tagaaaatat ggtatcaaag agataataaa aaaaaaaacg agggagtaca atggtaaaaa
      601 ttaacattac gatcgaagga gaagatgttt cagagattat caatgaactg ttacatatgg
      661 gtattgaatt attaaagaca aatagaaaag aaattcgaga agtgttaaag attcttgcgg
      721 atgaatcctt agagatctta aacgagaaga agacattgga gaaaggtgca gaactgtaca
      781 ggaaaattaa ctctgagatt aactggacct acaaacgaga taaagaataa acaccagaca
      841 gacatttatc ctaattcaat gaattcacgt gagctttttg taagaagctc caatccatct
      901 ttttttacga caacgtcgtc ttcaatacga actccaccga atcctggcag gtacacccca
      961 ggttcaacgg taaggaccat gttttctttc agttccatgg cattgtctgg ggcaaaccca
     1021 ataccatcat ggactgcgag gccaaggcca tgacccgtcg aatgaatgaa tgttcctttg
     1081 aattttgttg aatcgataaa cgaatgaact gcatgatgga catcacatgc ttttacccct
     1141 ggttttattg cctgaaacgc gatttgttgt gcttgaagaa ccgtctcgta catctctttg
     1201 tgttttccag tcgctttccc aaaaacaaac gtgcgggtga tatctgaatt gtatttttta
     1261 taacatgctc cgaaatcaca gagaacaacg gagccgttct cgagtttcac attcccatga
     1321 ccatagtgcg gttctgctgt gttcttccca aatgatgaaa tggtatcaaa cgctggtctt
     1381 tctgcaccaa ggctttgtag tgaataattg atttctgccg caagctcatc ttcgctcatc
     1441 ccttcagcaa cgatttcagg gattgtcacc atcgtctcat cagcgatttc acatgctttt
     1501 tttatgcgtt gaatttcctg ttcgtctttg atgagtcgtg atttgaaaaa tccatcagat
     1561 acatccacaa actttgtatt tggtacaagc cctgtgaact tgttgaaatc gagatatgaa
     1621 agaccacttg cgtttacacc aatggttgaa aagggagtaa tgagatgttt gagttctgtg
     1681 gaaaattctt cttttgtttt ataaatggct aaacgcgctg tggatttccg agcactttct
     1741 gcttcaagtt cagagaccaa aagatcgacg gttccgtcag aatggagtac tgcacaggat
     1801 ccttcaaaga gtccttgatc aagattcgtg acatagaaga agttatcatc gacatagggg
     1861 atggttgcat ttttaagtat gatacaatcg acagcctggc tgctgttacg aaaaacattt
     1921 ttgactctct gcatcacgta cgaatcaccc tacgttctta aacgagctcc tcacgtaaat
     1981 cgataaagaa gaagcgatgg gaatggtgta ttgaagacta cttttcgaat gaagtaataa
     2041 gattatggag ctggtctagg tcagttcgta tattctctac ttcttgcatc ttttcttttt
     2101 cgaagctttt caggatagat ggcaggtcag cggtaagctt gtagagatga accggtcttc
     2161 ctttcccttt ctttttttca tcgcgtttct tagcccagcc tctccggcgg agttcttgca
     2221 tagcgacgct aacttcaggt tgtcgaagat ctgctccttg ttcgacatct gcggagcgac
     2281 attcatccac ctgagaaatg tagagtaacg tctttgcgag attcttaggc attccaagtt
     2341 ctgagaacaa ctggactgct ttattttcgt tatcatccaa taccatagac tttttccgtt
     2401 tcatacattc tcctcgcgga ataaatcaat tctgaagtat atatattttt ccaatttttt
     2461 catttaaatt tttatatcct attctattat ttaaatcttt ttatttttag attgctttcg
     2521 ctcatcattt gtttcgcaag ctcatcagga tactcccctg cataaataat ttcagtgaca
     2581 ccagcattaa ttaacatctt gacacaaacg acacaggggg tattcgtaca atagagaaca
     2641 gaccctttaa ttgagacacc gaacaccgct gcttggatga ccgcgttttg ttctgcgtgt
     2701 aatcctctac agagttcatg ccgctctccg gaagggatat tctgatcctc gcgcagacac
     2761 ccagtttctg aacaatgtct gagacctttt ggtgccccat tatacccagt actcaagata
     2821 tgtttttctt ttacaagaat cgccccgact ttccgccgta aacaggtgct tcgctttgcg
     2881 accacataag ccatttccat gaaatattca tcgtaggatg gtctgacttc cataaaaatc
     2941 caaactacga atgattctgc agcttaataa aattattatt ctgcactact ctgagactca
     3001 ccatttcgtt tgaaaaataa atgatcccca agcactgccc ggaacactgt cgtaagaaga
     3061 gagaacccaa ggataatacc ccagattact ggatcaaata caggagcggt ggtaaacaga
     3121 gtattcaacc cagggacaaa aataatcgca agaatcatga ggaaggtaaa cacaaaaaaa
     3181 gatttcaaca gaatattttt tcgtttgaac ttctcgatga aagttccttc tcgtaagatg
     3241 aacacataaa tctgaggtga gagcaacgta atggcaaatg cgacagtacc tgccatcagt
     3301 cctgcctgct caagcccgat gccctgggct ttgaggtatt caaaacaaag gatgtaccct
     3361 gcagcgatgg ataagccgaa gatgatgcca tcgacaatca caattatccg atgcttacga
     3421 gaaataatcg gctctgacat cttagagggg tttcgtttca tgatattctt gtcagcagaa
     3481 tctgtactca ccccgatgct tgggaatgat tccatgatca cattcgacca gagcaattgt
     3541 aacggcatca acggcactgg atacccgaag agcggtgtaa caagaatacc gacgacctta
     3601 ccgatattgt ttgtgataag ataccggatg agtttcttca ggttctgata cac
//
LOCUS       DATMXJ010000234         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_46701, whole genome shotgun sequence.
ACCESSION   DATMXJ010000234 DATMXJ010000000
VERSION     DATMXJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46701"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VN377_06190"
     CDS             complement(<1..784)
                     /locus_tag="VN377_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR28006.1"
                     /translation="MSVRKTKHEMPEQLPKERIKNFKEVPYGYSKEIAIEEAKRCLQC
                     KNKPCMKGCPVEIDIPGFINCIAQGDFDKAADVIKAKNNLPAISGRVCPYEEQCEGEC
                     ALKKLGEPVGIGRLERFIADYEREKGKKPPKKSILTGKKIAVVGSGPAGLTCAGDLAR
                     MGYAVTVFEALHDPGGVLMYGIPEFRLPKAIVKSEVEYIKSLGVEINYDVVVGKTITV
                     QELMDEYDAVFIGTGAGLPNWLNIPGENLDGVYSANEFLTRVN"
     gene            complement(781..1602)
                     /locus_tag="VN377_06195"
     CDS             complement(781..1602)
                     /locus_tag="VN377_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HWR28007.1"
                     /translation="MYPIVEKKVLSENVKLLKIKAPLVAKKALPGQFIILRIDEKGER
                     IPLTIADSDPKNGTVSIIFLEVGKTTKQLGSLNVGDSLENFAGPLGMPSEVKKYGTVV
                     CVGGGVGIAPLYPIVKALKEAGNRVISILGAKTANLLLLEQEIHALSDEFYIATDDGS
                     KGHKGFVSDVLQQVIDKHKVDMVMAIGPIIMMKVVSTVTKKYNIKTLVSLNPIMVDGT
                     GMCGGCRVSIDGKTKFACVDGPEFDGHKVDYDNLMLRNRRFVHAEGDACKLVGAQ"
     gene            1745..2599
                     /locus_tag="VN377_06200"
     CDS             1745..2599
                     /locus_tag="VN377_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWR28008.1"
                     /translation="MNGIFVSDIHGNVPRFQRLFQIIRNEMPDAVFLGGDLLPLQLVK
                     HNTMEEFVETTIFAEIRNIQNDFDKKIRFFVIMGNDDPRMYEYIFIKADKAGILDYIH
                     QKTISYKKTYVTGYSYVPPTPFQLKDWERYDVSRYIDVGAVSPEQGKRTIDIPLDEIT
                     YSTIAEDLDTLSKQSPAKNTIYLFHAPPYHSCLDRADLHGKKVDHAPMDVHVGSIAIQ
                     RFIKTHQPLLTLHGHVHESAHITGRWKEKIERTYSFTAAHDGPELAVVRFDTHHLENA
                     TRELIPVS"
     gene            complement(2596..>3451)
                     /locus_tag="VN377_06205"
     CDS             complement(2596..>3451)
                     /locus_tag="VN377_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWR28009.1"
                     /translation="YTNLTKREQMQKVAKIISELNAKLPKRKFILMGPGRWGSKGDIK
                     LGVPVRYGDINNTTMILEIAKEKGGYIPELSFGTHFFQDLVESNIRYLPLYPDQGEDL
                     FNEKLLLESDNKLTDVLPSYKNYEDVVRVVKVSEIIPGGTLAVVMDGEANEALAYLKP
                     ADHLTWRTQKIEEIAQALDPELYGIQGMYLIGSTKDGSAGPTSDIDLLVHFKGTEEQK
                     DKLMVWFDEWGKKLAKENKERTGFATEGLLDVHIITDEDIKKKNSWASHITSPYQTVR
                     KISLKKDD"
BASE COUNT          930 a          804 c          643 g         1074 t
ORIGIN      
        1 tattcacacg ggtgaggaat tcatttgctg aatagacgcc atcgagattc tcaccaggga
       61 tattcaacca attcggaaga ccagcaccgg taccaataaa aacagcatca tactcatcca
      121 ttaactcttg aacggtaatg gtttttccta ccacgacatc atagttaatc tcaactccaa
      181 gacttttgat atattccact tcagatttta cgatggcttt agggagacga aactcaggaa
      241 tcccgtacat caacacacca ccagggtcat gtaatgcctc aaaaacggta accgcatatc
      301 ccattcgagc aagatcacca gcacatgtaa gacccgcagg accagagccg actaccgcga
      361 tcttctttcc cgtcaatatc gattttttcg gtggtttttt tcctttctca cgttcataat
      421 ccgcgatgaa tcgctccaat cgtccaatac cgactggttc accgagtttc tttaacgcac
      481 attctccttc acactgctct tcatacggac agacccgccc agagattgca gggagattgt
      541 tttttgcttt gatgacgtct gctgccttat caaaatcacc ttgcgcgata caattgataa
      601 accctgggat gtcgatttcc actggacatc ccttcataca cggcttgttt ttacactgta
      661 aacatcgttt tgcttcttca atcgctatct ctttgctgta gccataaggc acctctttga
      721 agttttttat ccgctctttc ggtagctgct caggcatctc gtgcttggtt tttcgtacgc
      781 tcattgggca cctaccagtt tacaagcgtc tccttccgca tgtacaaaac gacggttacg
      841 aagcattaaa ttatcatagt ctactttatg gccatcaaat tcaggaccat caacacaagc
      901 aaactttgtt tttccatcaa ttgagacgcg gcatcccccg cacattccag tcccatcgac
      961 cataattggg ttcaaactta ctagggtttt aatattatat ttttttgtca cggtggatac
     1021 aaccttcatc atgatgattg gaccgattgc catgaccata tccactttgt gtttatcgat
     1081 aacctgttgt agtacatcac ttacaaaacc tttgtgacct ttcgaaccat catcagtcgc
     1141 gatatagaac tcatcgctaa gcgcatggat ttcttgttct aataacaaga gattagcagt
     1201 tttcgctcct aggatggaaa tcactcgatt tcctgcttct ttcaatgctt ttacgatcgg
     1261 atacagtggt gctataccaa ctcctccacc aacacagaca acagttccgt atttttttac
     1321 ctccgatggc atccctagag ggcctgcaaa attttccaac gaatctccaa cattcagcga
     1381 accaagttgt ttcgtggttt ttcctacctc tagaaaaatg atactgactg ttccattttt
     1441 tgggtccgaa tctgcaatgg tcaaaggaat acgttcgcct ttttcatcga ttcgtaaaat
     1501 gatgaactga ccaggtaatg cttttttggc gactaacggg gcttttattt ttaagagctt
     1561 cacattctcg gataatacct tcttttcaac aatcggatac atagaaaccc tctgaagtta
     1621 ctttcttttg ttgtaactgt aattaggtat aattttaata gtttatgatg tctttaaacc
     1681 agagctaaaa tttattaaga tttaacagat ggctcgcata acgatccatg atgtggtgta
     1741 aaagttgaac ggtatctttg tttcagatat tcatgggaat gtcccacgct tccaacgtct
     1801 ctttcaaatc attagaaacg agatgccaga tgcagttttt ttgggtggag atcttcttcc
     1861 tctacaactg gtaaaacaca ataccatgga agaatttgtt gaaactacaa tttttgcaga
     1921 gattaggaac atacaaaatg attttgataa aaaaatccga ttttttgtta tcatgggcaa
     1981 tgacgatcca cgaatgtatg aatatatttt tatcaaggca gacaaggccg gcattcttga
     2041 ctatattcat caaaaaacta tctcttataa aaaaacctat gtcacgggat attcctatgt
     2101 acctccgaca ccgtttcagc tcaaggattg ggagcggtac gatgtctcac gatatattga
     2161 tgtcggagca gtatcgccag agcaggggaa acggacaatt gatattcctc tcgatgaaat
     2221 aacatattca acgattgctg aggatcttga cacactttca aaacagagtc cagcgaaaaa
     2281 cacaatttat ctctttcacg ccccaccata tcattcatgt cttgatagag cagacctcca
     2341 tggaaaaaag gttgatcacg cacctatgga tgtccacgtt ggaagtatcg cgatccaacg
     2401 tttcatcaaa acacatcaac cattattgac gttgcatggt cacgtccatg aatcagcaca
     2461 cattacagga cggtggaaag aaaaaattga aagaacgtat tcgttcacag ctgctcatga
     2521 tggacctgaa cttgcagtgg tacgattcga tacgcatcat ctggagaatg cgacaaggga
     2581 acttatccct gtgtcttaat catctttttt caatgaaatc ttccgcacgg tctgataggg
     2641 ggatgtgata tgggacgccc atgagttttt ctttttaata tcttcatcag tgatgatatg
     2701 gacatctaac aaaccttcgg tggcaaaacc ggttcgttct ttgttttctt ttgccagttt
     2761 tttgccccac tcatcgaacc acaccatgag tttatctttc tgttcctctg tccctttgaa
     2821 atgcacaaga aggtcaatat cacttgtcgg gccagcgctg ccgtcttttg tacttccgat
     2881 aagatacatc ccttgtatcc catagagctc agggtcaagc gcttgagcga tttcttcgat
     2941 tttctgcgtc cgccaggtga gatgatccgc tggtttgagg tacgccaatg cctcattagc
     3001 ctctccatcc atgacaaccg ctagagttcc tccgggaata atctccgaga ctttgacgac
     3061 ccgaacgaca tcctcatagt ttttatacga gggcagaaca tcggttagtt tgttatccga
     3121 ctctaataat aatttctcat tgaagaggtc ctctccctgg tctgggtata aggggaggta
     3181 cctaatgttt gattcaacta agtcttggaa gaaatgcgtt ccaaaggaaa gttcagggat
     3241 atatcctcct ttttctttcg ctatttccag tatcatggta gtattgttga tatcaccgta
     3301 ccgtaccggt acaccaagct taatgtctcc tttgctaccc cagcgtcctg ggcccatgag
     3361 aatgaatttt cttttcggaa gtttcgcgtt caattcactt atgattttag caaccttttg
     3421 catttgttct ctttttgtca agtttgtgta t
//
LOCUS       DATMXJ010000235         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_57086, whole genome shotgun sequence.
ACCESSION   DATMXJ010000235 DATMXJ010000000
VERSION     DATMXJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57086"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VN377_06210"
     CDS             complement(<1..971)
                     /locus_tag="VN377_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWR28010.1"
                     /translation="MRAIREYIKLIRPYGILFIGFTPVFGALCNGEFDVFHLSLLLLI
                     GLLGHIFVFVQNDYYDVEVDRQSKYVVQRPLTSGMISRTNAFIIFLGSFFISVVLASV
                     FLFSLRSFLFLLLSFFLMTLYNRYSKRKPGMEYVLGAAVFSYGMYGAFTVSDYVSLFA
                     VIISSVAFLQWVFSVGISANLKDVEFDTKLGIRTTPVIFGVQAIGNQLKKPVLFLLYA
                     YGIQLVHLIVVMFPFFFGYSSPMLVGYPIPLFVFLVLAGILLIITRGILTTPLDKRDQ
                     MLRYEGVHEGLALLLIPLVLLSYLVENLGALLPLIFLVVLIFWPLLC"
     gene            complement(987..1325)
                     /gene="queD"
                     /locus_tag="VN377_06215"
     CDS             complement(987..1325)
                     /gene="queD"
                     /locus_tag="VN377_06215"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWR28011.1"
                     /translation="MRLCREFYFDSAHFIPNYKGKCEKLHGHTYKLEIVIEGGVKKDG
                     MVMDFAKMKEIVETTVLEKLDHQALNELFENPTAEHILHWIAEQLQGKLPLSSMKLWE
                     GQGKWVEILL"
     gene            complement(1381..1860)
                     /gene="folK"
                     /locus_tag="VN377_06220"
     CDS             complement(1381..1860)
                     /gene="folK"
                     /locus_tag="VN377_06220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWR28012.1"
                     /translation="MPGVYLSLGSNIGDREAHITQAVHLISEICAVKKKSRFYLTEPV
                     GDIKQDWFLNCAIEVKTELDPKHLLSSIKSIEQTIGRIKTVKNGPRIIDIDILFYGDQ
                     IVQTKNLIIPHPLLQERLFVLQPMLDLNPDFVHPVLKKSILELYKNHPKTEKVLLYK"
     gene            complement(1860..2693)
                     /gene="folP"
                     /locus_tag="VN377_06225"
     CDS             complement(1860..2693)
                     /gene="folP"
                     /locus_tag="VN377_06225"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWR28013.1"
                     /translation="MSPLAPVICKEHTLRFQKTLIMGILNVTPDSFSDGGLFNNLDAA
                     VAHAKKMISDGADLIDVGGESSRPGSEPLTEKEELARILPVVTRLVDEVSVPISIDTY
                     KPVVADTCLKAGAHLINDITGLTDPLMRKIAAENNVPVVMMHMQGTPKVMQQNPAYQD
                     VIGDVKSFFQRQIVAAQKAGVRQIIIDPGIGFGKTVEHNLQILKHLGTFKTLGCPILV
                     GPSRKSFIGTITGLPVDDRLEGTIAAVTVAVMNGAHIVRVHDVKECRRAIQIVDAIRG
                     I"
BASE COUNT          866 a          604 c          597 g          778 t
ORIGIN      
        1 aaacagagaa gtggccagaa aatcaaaacg acaagaaata ttaatgggag taaggcacca
       61 agattttcaa cgagataact caagaggaca agcggaatca agagaagagc aagcccttca
      121 tgaacccctt cataacgaag catctggtcg cgtttgtcaa gcggtgttgt aagaatccct
      181 cttgtgataa tcagtaagat acctgcgaga acgagaaaaa caaaaagagg aatcggatag
      241 ccaacaagca taggagaaga atatccaaag aagaatggga acattactac gatcagatga
      301 accagttgta tcccgtacgc atacaaaaga aataagacag gttttttcaa ttgatttcca
      361 atcgcctgta caccaaagat tactggggtt gttctgatcc caagttttgt gtcaaattca
      421 acatccttta gattcgcaga gataccaaca ctaaagaccc attgcaggaa tgcgacgctt
      481 gaaatgatta ccgcaaataa tgaaacatag tcagagacgg tgaaagcccc atacatccca
      541 taggagaata ctgctgcacc taatacgtac tccattcctg gtttgcgttt gctgtatctg
      601 ttatacaggg tcattaagaa aaaagagagt aacaagaaaa gaaacgagcg aagggaaaag
      661 aggaagacgc tggcaagaac aacagatatg aaaaaagacc caagaaaaat aatgaatgca
      721 ttagttcttg atatcatacc agatgtgagt ggccgctgta caacatattt tgattgtcga
      781 tcgacctcga catcataata atcgttttga acaaagacaa aaatatgtcc cagtaacccg
      841 atgagtaaca acaaagagag atgaaatacg tcgaattctc cattgcacaa tgctccgaaa
      901 acaggagtaa aaccaataaa aagaatacca tatggtcgaa tgagttttat gtactcccgt
      961 atggctcgca cgatcccaaa tcctatttag aggagtatct ccacccactt cccctgtcct
     1021 tcccatagct tcatcgatga caagggtaac tttccctgta attgttcagc aatccaatga
     1081 aggatatgtt cagcagtcgg gttctcaaac agctcgttta atgcctggtg atcaagtttt
     1141 tcaagaacag tcgtctcgac gatttctttc atttttgcaa aatccatgac cattccgtct
     1201 ttcttcacac caccctcgat gacgatctct aatttgtagg tatgtccatg taatttctca
     1261 cattttccct tataattcgg gatgaagtgt gcagaatcga aataaaattc acgacacagt
     1321 ctcatgagat aaggctcctt tttcatgtaa aagaatagaa atgaagaata taaacgtcat
     1381 ctatttgtag agcaggactt tctcggtctt tggatgattt ttgtacaatt ccaggattga
     1441 tttttttagg accgggtgca caaaatctgg attcaaatcc agcatcggtt gaaggacaaa
     1501 aagccgttcc tgcaacaagg gatgagggat aatgagattt tttgtctgaa caatctggtc
     1561 tccataaaac aggatatcga tatcaataat cctgggtcca tttttcacgg tttttatccg
     1621 gccaatagtt tgttcaattg atttaatgga ggataacaga tgtttggggt caagttctgt
     1681 tttcacttca atagcacagt tcagaaacca gtcctgtttg atatctccga caggttcggt
     1741 gaggtaaaag cgagattttt tttttactgc gcatatctca gaaatcaggt gaacagcctg
     1801 agtgatatgt gcttctcgat caccgatatt tgacccaaga ctaagataga ctccgggcat
     1861 cagatacccc tgatcgcatc gactatttgg atggctcgtc tgcattcctt cacatcatga
     1921 acccgtacga tatgtgcccc attcatgaca gccacggtca ccgcagcgat cgtcccttcc
     1981 agacgatcgt ctaccggaag accagtgatc gtaccaatga atgacttccg agatgggccg
     2041 acaaggatcg gacagccgag ggttttgaac gtcccaaggt gtttcaaaat ctgcagatta
     2101 tgttctactg tttttccaaa accgatccct gggtcgatga tgatttgtcg tactccagct
     2161 ttttgcgctg ctacaatctg tctttgaaag aaagatttga catctccgat tacatcctga
     2221 taggcagggt tctgttgcat gaccttcggg gtcccttgca tatgcatcat gacgacaggc
     2281 acgttatttt ctgccgctat cttcctcatg agcgggtctg tcaatcctgt gatatcgtta
     2341 ataagatgtg ctcccgcttt gagacaggtg tctgcgacaa ctggtttata ggtgtcgata
     2401 gaaatcggga cagaaacctc gtcaaccaat cgtgtcacca ctggtaagat cctggcgagt
     2461 tcttcttttt ctgtgagagg ctccgaacct ggtcgtgagc tttccccacc aacatcgata
     2521 aggtctgctc catcggaaat catttttttc gcatgagcga ccgccgcatc aagattattg
     2581 aataatcctc catcggagaa cgaatcaggg gtgacattga ggattcccat gatcagggtt
     2641 ttttgaaatc gcaaggtgtg ttccttgcag ataaccggtg caagcggtga cattatttcc
     2701 ctaaaatgag gacgacctat taatactcaa tgtttccaca catagagctt cttaaaatct
     2761 catgaaacct ttcggtcggg agaataatgg ggtggtagga aaaaatctct ttttttgtaa
     2821 aagtttattt ttataagcaa tatac
//
LOCUS       DATMXJ010000236        10024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72059, whole genome shotgun sequence.
ACCESSION   DATMXJ010000236 DATMXJ010000000
VERSION     DATMXJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72059"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VN377_06230"
     CDS             <1..304
                     /locus_tag="VN377_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28014.1"
                     /translation="QHFKCPSCGGIFAIKKSNQNNKKPFTLTCPDCGMAGFVPSKPTR
                     IQQEIPEKKSVHKNFQCQNCGEWVMVWAEGVNMIQDQNIHVYSCPYCGEKQALHPI"
     gene            complement(301..2139)
                     /gene="arcS"
                     /locus_tag="VN377_06235"
     CDS             complement(301..2139)
                     /gene="arcS"
                     /locus_tag="VN377_06235"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF040592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HWR28015.1"
                     /translation="MDFQVKIRDGPARIGLFTQNNETIRTPNIFYIQTTRCKAPAFSD
                     VLLTNKTLKNKKPTIQIGESIFSTTKNKKEKNLCLGNYLIYPKDVTTELHVSALQQNR
                     DTECRVIPAKKELIQYLDNKDDAILFIVSNATQLFSQQSFFVDFITALRQKIGYTKPI
                     YVPCVGDPTSIALLTYMSVDFFDSLSAIMAARNEIFLLPTGFYRKNQLPEIPCSCPTC
                     VTTDDVSSMGYDEILQHNYSTLFSEIKLVRNAISSGSLRELVEMRVRTSPHLAALLHI
                     LDLNQYDFLEERTPIVRKQPLIATTKDSLVRPEIRRFQDRVIDRFEKPTSAKILLLLP
                     CSAKKPYSFSKSHKLFRECFLNLVNPFVVHEVILTSPLGLVPRELELMYPASNYDIAV
                     TGHWDENEKLMIRTLIQKYLEKNTYDATIMHIPPAMQAFVRDLLKNPRITCTNSPTSA
                     ECLTALSNMLQELTSGYDHVKPRRRVYENIMSLACYQFGPALAKRLMRDCTISGKYPY
                     QKIMDGNTQLGMLTEERGLLSLTMDGANRLGETEQYWVEISDDFTLKGSVFAPGIQDA
                     DDSIRIGDEVLVKKKDRLCGVGVALMNGKEMTESTHGEAVKIRHHT"
     gene            complement(2185..3735)
                     /gene="purH"
                     /locus_tag="VN377_06240"
     CDS             complement(2185..3735)
                     /gene="purH"
                     /locus_tag="VN377_06240"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWR28016.1"
                     /translation="MEYRSALISVYDKEGIVDFSKGLIKLGIRILSTGGTADVLEKAG
                     IAFTEVSTVTGFPEMLDGRVKTLHPIIHAGILARRDKKEHQKTLKDHHIDWIDVVVCN
                     LYPFEKTIQKPKVSIEEVIENIDIGGPTLIRAAAKNYADVVVVTSPKQYPEILTALQT
                     KIGITQEHREALAIQAYAHTAQYDTMIAHYLRNRWTEELLPQDFSVTMRKIQEMRYGE
                     NPHQKGAFYQGLPPIEEPCIATTEQLQGKELSFNNILDANCAIECIKEFRKPSCVIIK
                     HATPCGIATASTLLQAWHDAYATDIYSPYGGIVAFNGELQKDVAQELSKYYLEVLVAP
                     SFAKEAQMMLHEKKNLRLLQLHGLEKEHTRKGLDIRSVVGGFLIQERDVWFADQKTWK
                     VVTTTKPSREDLVSMDFAVKCVKHIKSNSVVFVKDTRTVGIGGGQTSRVDAAWIATYK
                     GKENIKGSIMASDAFFPFRDAVDVAVNAGVRAIIQPGGSIRDTEVIQAANEHKIAMVF
                     TGQRYFRH"
     gene            complement(3814..4575)
                     /locus_tag="VN377_06245"
     CDS             complement(3814..4575)
                     /locus_tag="VN377_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28017.1"
                     /translation="MKTIGVYTKDFSLYHDIIKGLKKRNVAYVSLTSSEDLPRRIGVI
                     LTSQKELPRIALSKKVAADAYDSIDYAIDLAVQKLTGKELYSKIYIGIDPGERPGIAI
                     VCDDILLQKIQVDSPERVVAEVKRFLKVYQSKETCIRIGHGSVINRNRIINSLIPLEK
                     PIEIVDETRTTPAQQKRRCVRDSEAAAAIALLSGGRVQSTLPIETSRGAIKNIQRWSR
                     QLSEGRFTISEKTALRVLTGELSLLEAIEQEKKPL"
     gene            complement(4623..5363)
                     /locus_tag="VN377_06250"
     CDS             complement(4623..5363)
                     /locus_tag="VN377_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWR28018.1"
                     /translation="MSERQKFYTSFHDLSPREYMYIPEGSLQVGKPGSFSRIEVGHLI
                     IAIVVLTLAFSFTFSKNNLLFFLLGSPIDIGSYFFFMPIAFLGIITAFFIHELSHKFV
                     AQKCGLWSEFRMYPRGLILSVVVAFFTGLVFAAPGAVMFQGTAKPSETGRIAAAGASA
                     NIVIAVITYMLYHFFFSQMGFWGTLVGFICLVNLLLATFNLLPFGPLDGKKVLVWSEM
                     VWFVLFSVSVLLLSGMFIGGNLLTKFFT"
     gene            complement(5413..6582)
                     /locus_tag="VN377_06255"
     CDS             complement(5413..6582)
                     /locus_tag="VN377_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HWR28019.1"
                     /translation="MEEKITDNIILIGTAHISEESVLEVRKAIETYKPDIVAVELCQR
                     RYETITKKDKWENTPVTALIKSNNAYFMLAQTFLSSIQRRLGEEYGVEPGSEMIAAMQ
                     EAEKYHLTVALVDRDITVTLKRAWRMMGVREKFRVVWEFLKAMIGYDEEELEELDLKE
                     MMKQDVLSQMMEEFSKIAPSASKVLISERDQYIAQKIYEESKKGKVVAVVGAGHINGI
                     KKHLENQELNADLHELEQIPKKRFNLVKVIEVAIPVIFFGVIGWIIYSTGMNPWNKIK
                     DIFLIWFLVHGILSGVGVAIARGHPLSILTAFLAAPFTSLEPFFAPGWFAGLVEAKLR
                     KPLIKDFQELSKIENIRGFLRNKVVRLLMVVALANLGSIIGTIIALPWILSLGLG"
     gene            complement(6632..7258)
                     /gene="rpsB"
                     /locus_tag="VN377_06260"
     CDS             complement(6632..7258)
                     /gene="rpsB"
                     /locus_tag="VN377_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWR28020.1"
                     /translation="MSEEKQPVETEQTMMVTEEIYMTSGVHIGTRQKTADIKEFIYKV
                     RNDGLYIIDVKKTDERIKATAKFLANYDPAKILVVSVRQYGQKPVRKLGETTGIQVLD
                     GRFQPGTLTNPNAKTFLEPEIILLTDPLADIQALHEAKNIGIPVIALCDTNNETKYID
                     YVIPTNNKGRRALALIYWLLARAILREQGKIQSDEEFTTTVEDFEAEI"
     gene            complement(7274..7459)
                     /locus_tag="VN377_06265"
     CDS             complement(7274..7459)
                     /locus_tag="VN377_06265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HWR28021.1"
                     /translation="MTYTRFEKARIIGARALQLSMGAPTILAEIPKDMIDPVEIAMLE
                     YDENAIPITVKQKEIKA"
     gene            complement(7579..7797)
                     /locus_tag="VN377_06270"
     CDS             complement(7579..7797)
                     /locus_tag="VN377_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28022.1"
                     /translation="MEEVLYNIEIHRNDGNYMGKIYSDIDGVKEFENNRIDRLLRDII
                     VDMQLALDDEFSSRMTATDIPEFTDEIK"
     gene            complement(7875..8339)
                     /locus_tag="VN377_06275"
     CDS             complement(7875..8339)
                     /locus_tag="VN377_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWR28023.1"
                     /translation="MVYAVIRVRGTVNIKPDIKKTMQMLRLTRANHCVLLEENAVYKG
                     MLQMVKDYTTWGEINKETLAKLLSTRGKLVGDKPLTEEYLKKAAPYHSFEKLSEAIIE
                     NKVNFKDIPEVKPLFRLNPPKKGHRTVKRSFVNKGSLGYRKDAINTLIERML"
     gene            complement(8344..9096)
                     /locus_tag="VN377_06280"
     CDS             complement(8344..9096)
                     /locus_tag="VN377_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWR28024.1"
                     /translation="MAGRERGNAPRGRRRQFDEGAAPVIWAPKTRLGKMVKNGEITSM
                     GDALTSGLPLREPEIVDLLLPSVEDEVIDVKMVQRMTDSGRRVKFVITVAVGNKDGFV
                     GLGQAKGKEVGLSIKRAIENAKMNMIQIRRGCGSWECGCRTPHTIPFLVTGKSGSVEV
                     TFKPAPRGIGLATGDVAKKILRLAGVNDCWAFTEGKTKTVVNYAKGVYDALKKNAEMR
                     INKNEMSKLGIVQGSIGIPVKEEQAAPVKTEG"
     gene            complement(9099..9611)
                     /locus_tag="VN377_06285"
     CDS             complement(9099..9611)
                     /locus_tag="VN377_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWR28025.1"
                     /translation="MKSGPRYHVKPRRHRERKTDYRLRLKLLRSKKPRIVVRKSLKAI
                     RVQFVEYNPQGDRILASAVSNELAKDYGWKYSASNTPAAYLTGVLAGKRAKEKGIEHG
                     VLDIGLYHPTVGSTLFASLKGVLDAGIECPHDAQMLPKDERIYGVHLNKEIKPLVDDI
                     KTKIIGVKEE"
     gene            complement(9608..>10024)
                     /locus_tag="VN377_06290"
     CDS             complement(9608..>10024)
                     /locus_tag="VN377_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HWR28026.1"
                     /translation="KCGGTRVWMDPDRLEEIAKAVTRNDVVILVKGGAIKSIQKQGNS
                     RGRHKYVLNQRKKGRRTGQGSRKGPKHARLPKKEQWIRTIRPIRTYLKELRQGTKIDA
                     HTYRKYYRQAKGGVFRSKQHLKSHLLSDNIIKEEKQ"
BASE COUNT         2517 a         2405 c         2088 g         3014 t
ORIGIN      
        1 acagcatttc aaatgcccat catgcggcgg catcttcgct atcaaaaaat caaatcagaa
       61 caacaaaaaa cccttcacat taacatgccc tgattgcggg atggccggat ttgtcccctc
      121 gaaaccgacg cggatacaac aggaaatccc tgagaagaaa tcagttcata aaaatttcca
      181 gtgtcaaaac tgcggggaat gggtcatggt ttgggctgaa ggcgtcaaca tgatccagga
      241 ccagaatatt catgtgtatt cctgccccta ttgtggtgaa aaacaagctc tacatcctat
      301 ttaagtatga tgccgtattt ttactgcttc tccatgtgtc gattctgtca tttcttttcc
      361 gttcatgagt gctacaccca cgccacaaag ccgatccttt ttcttcacga ggacttcatc
      421 gccaatccgt attgagtcat ccgcatcttg tatccctgga gcgaataccg atcccttcaa
      481 tgtgaaatca tcagagatct ctacccaata ctgttctgtt tctcccagtc tgttcgcacc
      541 atccatagtt aaggaaagta gccctctttc ttctgtcaac atccccagct gcgtgtttcc
      601 atccatgatc ttctgatagg gatatttccc actgatcgta caatctctca tgaggcgctt
      661 cgcaagagca ggaccgaact gataacacgc aagtgacatt atattttcat aaacccgtcg
      721 tcgtggcttg acatggtcat agcctgatgt tagttcttgt aacatattcg ataatgcggt
      781 aaggcattca gctgatgtag gggaattcgt acaggtgatc cgtggatttt tcaaaagatc
      841 ccgtacaaat gcctgcatcg ctggagggat atgcataatc gtcgcatcat aggtgttttt
      901 ctccaggtat ttctggatga gtgttcgaat cattagcttt tcgttttcat cccaatggcc
      961 agtcaccgca atatcatagt ttgacgcagg gtacatcagt tcaagttctc ttgggacaag
     1021 accaagtggt gaggtcagga tcacttcatg aaccacaaag ggatttacca ggttcaagaa
     1081 acattccctg aagagtttat gggatttgga aaacgaatac ggttttttcg ctgaacaggg
     1141 tagcaggagt aatattttcg cactcgttgg tttttcaaaa cggtcgatga cccgatcttg
     1201 gaatcgtcta atctcgggtc gtacaagaga atctttggtg gttgcaatca atggttgttt
     1261 tcgaacaatc ggggttcgtt cttctaaaaa gtcatactga ttcagatcaa gaatgtggag
     1321 taaggccgca agatgcgggc tggtacgtac acgcatctcg acaagttctc gtaacgaacc
     1381 agaggaaatc gcgttcctca ccaatttaat ctcagagaac agcgtagagt aattatgctg
     1441 caagatctcg tcatacccca ttgaagaaac atcgtctgtt gttacacagg ttggacagct
     1501 gcagggtatc tctggaagct ggttttttct gtaaaaacca gttgggagca ggaatatctc
     1561 atttcgtgct gccatgattg cggataatga atcaaaaaaa tcaacgctca tgtatgtaag
     1621 cagagcgata ctggtcgggt caccaacaca tggaacatag atcggcttcg tatatcctat
     1681 tttttgtcgt aacgctgtga tgaaatcaac gaaaaatgat tgttgagaaa acaattgagt
     1741 cgcattggag acaataaaaa ggatcgcgtc atccttgttg tccagatatt gtatcagctc
     1801 tttttttgca gggatgacac gacactcggt gtctctattc tgttgaagtg cacttacgtg
     1861 taattcagtg gtgacgtctt ttggatagat gaggtagttt cccaaacaga ggttcttctc
     1921 tttcttgttt tttgttgttg aaaagatact ctcccctatc tgtatcgtag gttttttgtt
     1981 tttcagagtc ttatttgtca ataagacatc ggagaatgct ggtgctttac atcgggttgt
     2041 ctgaatgtaa aagatgtttg gggttctaat cgtctcgtta ttctgtgtaa agagtccgat
     2101 acgagcaggt ccatctctaa ttttaacttg aaaatccatt agtcactgat aatatgttct
     2161 ctttaagaac atttagtttc ctggttaatg ccggaagtat ctctggccag tgaacaccat
     2221 cgctatctta tgttcgtttg cagcctgaat cacttcggta tccctgatgg acccaccagg
     2281 ttgaatgatt gctctcaccc ctgcgttcac cgcgacatcc accgcatccc ggaaggggaa
     2341 gaacgcgtcg cttgccatga tcgatccttt gatgttctct ttccctttat aggtagcaat
     2401 ccaggctgcg tcaacacggg atgtctgtcc tccgcctata ccaaccgtcc gtgtgtcctt
     2461 gacaaacacc acagagttcg atttgatgtg tttgacgcat tttactgcaa aatccatgga
     2521 aaccaggtct tcacgtgatg gtttcgtagt tgtgacgacc ttccaggttt tctggtccgc
     2581 gaaccagacg tctcgttctt gaatgagaaa tcctccgacg acactgcgga tatccaaccc
     2641 tttccgcgta tgttcctttt ccagcccgtg aagctgtaat aatctgagat ttttcttttc
     2701 atggagcatc atttgagcct cttttgcaaa gcttggtgcg actagcactt caaggtaata
     2761 ctttgaaagc tcctgagcga catctttttg tagttcgccg ttaaacgcga cgattccacc
     2821 atagggagaa tagatgtcgg tggcgtacgc atcatgccag gcttgaagca acgttgatgc
     2881 agtcgcgatc ccgcaggggg ttgcatgttt aatgatcaca caagaaggct ttctgaattc
     2941 cttgatacat tcaatcgcgc agttcgcatc caggatgttg ttaaacgaga gctctttgcc
     3001 ttgcagctgt tctgtagtgg cgatgcaagg ttcttcaata gggggaagac cttgataaaa
     3061 cgcgcctttc tgatgcggat tttccccata acgcatctcc tggatttttc gcatggtgac
     3121 tgaaaaatcc tgtggtaaaa gctcctcagt ccaacgattt ctcaaataat gagcaatcat
     3181 cgtatcatac tgggcggtat gtgcatatgc ttgaatggct aacgcctcac gatgttcctg
     3241 ggttatacct atttttgttt gtaaggcagt caggatctct gggtattgct ttgggctggt
     3301 gactacgaca acatcggcgt agttcttcgc agcagcccgg atgagggtgg gtccaccgat
     3361 atcaatgttt tcaatgacct cttcgatact gacctttggt ttttgtatgg ttttctcaaa
     3421 aggatacagg ttacatacca caacatcgat ccaatcgatg tgatggtctt tcagggtttt
     3481 ttggtgttct ttcttatcgc gtcgagcaag aataccagca tgaataatcg gatgaagggt
     3541 cttcactctc ccgtcaagca tctcaggaaa cccagtcacc gtggatactt ctgtaaatgc
     3601 gattcctgct ttttccaaaa catcagcggt accaccggtt gatagaatac ggattcccag
     3661 ctttatgagt ccctttgaaa aatcaacgat gccttcctta tcatagacac ttatcagtgc
     3721 agatcggtat tccattactc ttacccaagg aacatatgtc cgatactcat aaacatttgg
     3781 gtcgacccag ttgctccgac gatacgaaac cagctagagt ggtttttttt cttgttcgat
     3841 cgcttccaac agactcaact ccccagttaa gactcgcagc gcggttttct cagagatcgt
     3901 aaatctccct tcactgagct gcctgctcca tctctgaatg ttcttaatcg ccccacgaga
     3961 ggtttcaata gggagcgttg attgcacccg accaccagac aacaaggcga tagcagcggc
     4021 tgcttcacta tcccggacgc atcgtcgttt ctgctgagct ggcgtcgtcc gcgtctcatc
     4081 cacgatctcg ataggcttct ctaaagggat cagagaattg atgatgcgat tcctgttaat
     4141 caccgacccg tgtccaatcc ggatacaggt ctcttttgac tgatacacct ttaaaaaacg
     4201 tttcacctca gcaacaaccc tttcagggct gtccacctgg attttctgca agaggatatc
     4261 atcacacacg atcgctatcc ctggtcgttc ccctgggtca ataccgatat agattttcga
     4321 gtacagctcc tttccagtca gtttttgtac agcgaggtca atcgcataat caatcgaatc
     4381 ataggcatct gcggcgactt tcttacttaa agcaatccgg ggtaactctt tttgcgatgt
     4441 cagaataaca ccgattcttc ttgggaggtc ctctgatgat gtcaaagaga catacgcgac
     4501 attccgcttt tttaatcctt tgatgatatc atgatataac gaaaaatcct tggtgtacac
     4561 acctattgtt ttcatccaaa aaaactatcc gatatgttga atatatacgt ttcccccctg
     4621 tattacgtaa aaaacttcgt cagcagattc ccgccaataa acatcccgct gagcaataac
     4681 actgaaacag aaaacaatac gaaccacacc atttcactcc acaccaacac tttcttccca
     4741 tccaacggac caaagggcaa taaattaaat gttgcgagca agagattcac caagcagata
     4801 aatccaacga gagtccccca gaaccccatc tgagaaaaga agaaatgata caacatatac
     4861 gtgatcactg cgataacaat attcgcactg gctccagcag cagcgatcct gccggtctcc
     4921 gatggtttcg cagtcccctg gaacatgact gccccggggg cggcaaacac aaggccggtg
     4981 aagaaagcga caaccactga gaggattaat ccgcgtgggt acatacgaaa ctcagaccat
     5041 agcccacatt tttgtgcgac aaatttatgg gaaagctcat ggataaaaaa tgcggtgatg
     5101 atccctaaaa aagcaattgg cataaaaaaa aagtaagacc caatatcaat cggtgacccc
     5161 agtaagaaaa aaagaagatt gttctttgaa aaggtaaaag aaaacgctaa ggtaagcact
     5221 acgattgcga tgatcagatg accaacttca atcctgctga acgaccctgg tttaccaacc
     5281 tgcagactcc cttcagggat gtacatatac tcccgggggg acagatcatg aaacgaggtg
     5341 tagaacttct gtcgctcaga cataatggtc gtaccgattg atacgagtta taaggaatct
     5401 ggataaaaac atttagccaa gtccgagact caggatccac ggcaacgcaa tgattgtacc
     5461 aataatgcta ccgagatttg ctaaggcgac gaccatcaga agacggacga ccttgtttct
     5521 taaaaaccca cgtatatttt caatcttgct gagttcctga aaatccttta tcaatggttt
     5581 ccgtagtttg gcttcgacca atccggcaaa ccatcccggg gcgaagaatg gttcaagtga
     5641 tgtaaaaggg gcagccaaaa aagcagtaag aattgataac gggtgacccc gggcgattgc
     5701 tacgccaaca ccagagagaa tcccatgcac aaggaaccat atcaggaaga tatccttgat
     5761 cttattccat gggttcatcc ctgttgaata gatgatccag ccgattacac caaaaaaaat
     5821 cacggggatg gcaacctcaa tgacctttac aagattgaag cgtttctttg ggatttgctc
     5881 taactcgtgc aggtcagcat tcaactcctg attctccaga tgtttcttga taccgttgat
     5941 atgtccagca ccaaccactg cgacgacctt tcctttctta ctctcctcat agattttctg
     6001 cgcaatatat tgatcccgtt cagaaatcag tacttttgaa gcgctaggtg caatcttact
     6061 gaactcctcc atcatctgag aaagaacatc ttgtttcatc atttctttca aatcaagctc
     6121 ttcgagttct tcttcatcat acccaatcat tgccttaagg aactcccaga ccaccctgaa
     6181 tttctcacga actcccatca tacgccaggc acgttttagc gtcaccgtga tatctcgatc
     6241 gaccaaggcg accgtcagat ggtatttctc cgcctcttgc atagcagcga tcatctcaga
     6301 gccaggttcc acgccgtact cctcgccgag tctccgttgg atcgaagaaa ggaaggtttg
     6361 tgcaagcata aaatacgcgt tattggattt gatgagcgcg gttactgggg tgttttccca
     6421 tttgtctttt tttgtaatcg tttcataacg acgttgacac agttcgacag caacaatatc
     6481 tggtttatat gtttcaatgg ctttccttac ttcaagaaca ctctcctcag aaatatgagc
     6541 ggtgccgata aggatgatgt tgtcagtgat tttttcttcc aaaggtatct cctttgagct
     6601 gtttacatga gcgacgactt caaaaaatta tttaaatttc tgcttcgaaa tcctcgacag
     6661 ttgtagtgaa ttcctcatcg gactgtatct tcccttgttc ccggaggatc gctcgagcga
     6721 gcaaccagta gatcagggca agcgcacgtc gacctttgtt attcgttggg atcacataat
     6781 caatgtattt tgtctcgttg ttcgtatcgc acaaggcgat gacaggaatg ccgatgttct
     6841 ttgcctcgtg caatgcctgg atatcagcga gcgggtctgt aagcaaaatg atttccggtt
     6901 caaggaatgt tttcgcattc ggattggtca gtgtccctgg ctggaatcga ccatcgagca
     6961 cttggatccc ggtcgtttcc ccaagctttc ttaccggttt ttgcccgtat tgacggactg
     7021 atacaacaag tattttcgct ggatcgtaat tcgcaagaaa cttcgctgtt gcctttatcc
     7081 gttcatcggt tttcttcaca tcgatgatgt acaatccatc gtttcgtact ttgtagatga
     7141 actctttgat atcagcagtt ttttgcctgg tgccaatatg gacgcctgat gtcatgtaaa
     7201 tttcttcagt caccatcatg gtttgttctg tttctacagg ttgtttttct tcagacatag
     7261 tcgtgcctca aatctatgct tttatttcct tttgtttcac cgtaatggga atagcatttt
     7321 cgtcatactc aagcatcgcg atttcaacgg ggtctatcat atctttcggt atctccgcta
     7381 agatcgtggg tgcccccata gagagttgta aggctcttgc gccaatgata cgagcttttt
     7441 caaagcgagt atacgtcaca gtcatcctcc gaaacatcgg gataggtaga agcccaaata
     7501 acagaaacct ttaagaatat tgcccttaaa tacccctgtt tttcaagcgt actatggttt
     7561 ttacagaaca gacgggtact attttatctc atcggtgaac tcaggaatat ctgtcgcggt
     7621 cattctgcta gagaactcat catctaacgc aagctgcata tcaacaatga tatcgcggag
     7681 taaacggtcg attctgttgt tctcaaattc ttttacccca tcaatgtcag aataaatttt
     7741 ccccatataa ttcccatcat tacgatgtat ttcaatattg tagagaactt cctccatctt
     7801 ctaacaccct cttttccttt attcacccaa taaaatagaa tcactataat aatcttttgg
     7861 aatatttcgt ttgcttataa catccgttca atgagggtat tgatcgcgtc ttttcggtac
     7921 cccaaagagc ctttgttcac aaacgatcgt ttcacagtcc ggtgaccttt ttttggtggg
     7981 ttgagtcgga acaatggttt gacttccggg atgtccttga agttcacttt attctctatg
     8041 atcgcttcag agagtttttc aaaactatga tacggcgcag cctttttgag gtactcttcg
     8101 gtaagcggct tatcaccaac gagttttcct ctcgtggaca atagttttgc gagtgtttct
     8161 ttgtttattt caccccaggt agtgtaatcc ttcaccatct gaagcattcc tttgtagaca
     8221 gcattttctt ctaaaagcac acagtggttc gcgcgtgtta accgcagcat ctgcattgtt
     8281 tttttaatgt caggttttat attcaccgtg cctcttactc tgattactgc atacaccatg
     8341 gatttatccc tccgttttaa ccggggcagc ttgttcttct tttacgggta tcccgatact
     8401 cccttgaacg ataccgagtt tactcatttc attcttgttg atcctcatct ctgcgttctt
     8461 tttcaaggca tcatagacac cctttgcata gttgacaact gtctttgttt ttccttcagt
     8521 aaacgcccag cagtcgttga ctccggcgag gcggaggatc ttctttgcga catcaccggt
     8581 cgcaagcccg ataccgcgag gcgctggttt aaaggtgact tcaaccgagc cgctttttcc
     8641 cgtgactaaa aatggaatcg tatggggggt ccggcatccg cattcccagg atccgcaccc
     8701 gcgtcgtatt tgaatcatgt tcatctttgc attctcaata gctcttttga tgcttaaacc
     8761 gacttctttc ccctttgctt gaccaagacc aacgaaaccg tctttgttgc cgaccgcaac
     8821 ggtgatgacg aacttgactc gtcgccctga gtctgtcatc cgctgtacca tcttgacgtc
     8881 gatgacttca tcctcgacgc ttggtaagag aagatcgacg atctcaggtt ctcgcaaagg
     8941 caacccggat gttaacgcat cgcccatgct cgtgatctcg ccgtttttca ccatttttcc
     9001 aagtctggtc tttggtgccc atatgacagg ggcggctccc tcatcaaatt gtctgcgtcg
     9061 tcctctgggt gcatttccac gttctcgtcc agccatactt attcctcttt gactcctatt
     9121 atctttgttt tgatatcatc aacgagcggt ttgatctctt tgttgaggtg tactccatag
     9181 attctttcat ctttcggcag catctgtgca tcatgggggc attcaatccc agcgtccagc
     9241 actcccttca atgaagcaaa caatgtactg ccaaccgtgg gatgatataa accaatatct
     9301 aaaacaccgt gttcgattcc tttttctttt gccctctttc cagcaagaac accagtaaga
     9361 tacgctgccg gtgtgtttga agcagagtat ttccaaccat agtcttttgc taattcatta
     9421 gaaacggcgg atgcaagtat cctgtcaccc tgcgggttat actcgacgaa ctgcacacga
     9481 atcgccttga gtgattttct gacgacgatg cgcggtttct tgcttcggag taatttcagt
     9541 cgtaagcggt aatcggtttt tcgttcacga tgtcttcgtg gcttgacgtg atatcgtggt
     9601 cctgatttca ttgtttctcc tccttgatga tattatccga gagtagatgt gatttcaaat
     9661 gttgtttact tcggaagaca ccgccttttg cttggcgata atatttccga tacgtatgtg
     9721 catcgatctt tgtaccttgc cgtaattctt tcaagtacgt ccgaattggg cgaatcgtcc
     9781 ggatccattg ttcttttttc ggaagtcgtg cgtgtttcgg cccttttcgc gatccttggc
     9841 cagtccgtcg tcctttcttc ctctgattga gaacatattt gtgtcgcccc cgggaattcc
     9901 cttgtttttg gatacttttt atggcgccac ctttcactaa aattaccaca tcatttcgtg
     9961 ttactgcttt cgctatctct tcaagtctat ctgggtccat ccaaactctt gttccgccgc
    10021 attt
//
LOCUS       DATMXJ010000237         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36101, whole genome shotgun sequence.
ACCESSION   DATMXJ010000237 DATMXJ010000000
VERSION     DATMXJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36101"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1830
                     /locus_tag="VN377_06295"
     CDS             43..1830
                     /locus_tag="VN377_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR28027.1"
                     /translation="MKKNMKSFIGAVFCLLLVIVVPGTIPGQINQTQPTGSTEMSRAF
                     LSGTISQIETIGGQTYAHAIRVLYFGTGGIGLMKDGQISYQSDKFLRHFYMIPRGSAT
                     RIFGFVHNFDTAPGGQVPPEILQYQGDWPMANKDYQNTRATKDSIITSSNVASLSLGW
                     SYAIQGIGAYGGAASTPLIVGNTVLFQDLKGNVVALDRQTGNVIWTKWYNDTAIVGPD
                     GPAVGYGNVYVAKDLYNITAINMSTGTEVWVTRISYVATTGIDIQPIVYDNIVFISTV
                     PGTGDVFYAPGGIGVIYALDAQTGSILWNFSTIDSPDLWGHPEVNSGGGCWYPPAIDR
                     ATNTIFWAISNPAPFSGTVEWPNGSSFTGPALFTNCMMALDYRTGKMKWFSQAIAHDI
                     FDHDLQISPILANATISNVEHQIVIGAGKMGNVYAFDRETGAILWTVPVGKHENDTLD
                     RLSGETLVYPGILGGVETCMAYSDGIVFVPVVDLSTKYLPTGLNYSSINFAEGKGELV
                     AIDVNLGKILWYKTFDSINVGAATVVNDVVFTATFTGMIYAFQAQTGQQLFSYQAPAG
                     INAWPAIAGNMILWPTAVGGTPSLIALHV"
BASE COUNT          596 a          412 c          477 g          596 t
ORIGIN      
        1 gttatatact ttgggagaat tcatgatgaa gaggatataa atatgaaaaa aaacatgaaa
       61 tcgtttattg gagcagtatt ttgtttgctg ctcgtaattg tagtcccagg tacgattcct
      121 ggacagataa atcagacaca accgacagga tcaacagaaa tgtccagggc gtttctgtct
      181 gggacgattt ctcaaataga aaccattggt ggccagacct atgctcatgc aataagagtt
      241 ctttattttg gtactggtgg aattggtttg atgaaggatg gtcaaattag ttaccaatct
      301 gataagttcc ttcggcattt ttatatgatt ccaagaggtt cggctacccg gatatttggt
      361 ttcgttcaca attttgacac agcacccggg ggtcaggtgc cacccgaaat ccttcaatat
      421 cagggagact ggccaatggc gaataaggat tatcaaaata cacgggctac gaaagactca
      481 attattacat ctagtaatgt cgcgtcactt tccttaggct ggtcgtacgc aatccaagga
      541 attggtgcat atggtggcgc agcaagtacc cccctcattg ttggtaacac tgtgttgttc
      601 caagatttaa agggaaatgt ggttgccctt gatcgacaaa caggaaacgt catttggacg
      661 aaatggtaca acgatacagc aattgtcggt ccggatggac ctgcggtcgg ttacgggaat
      721 gtatatgtcg caaaagattt gtacaatatc actgccatca atatgagtac tggtaccgaa
      781 gtatgggtga caagaatttc ctatgtggca acaacaggga ttgatatcca accaattgta
      841 tatgacaaca ttgtgtttat atcaacagtt cctggaaccg gagacgtctt ttatgctcct
      901 ggcggaatcg gtgtgatata tgccctcgat gcacaaaccg gatcgatttt atggaatttc
      961 agcaccattg actcaccaga tctctgggga catccagaag taaacagtgg tggtggatgc
     1021 tggtatcccc cagcgatcga tagagcgaca aatacgatat tctgggcgat ttcaaatcca
     1081 gccccgtttt ctggaaccgt ggaatggccg aatggatcaa gctttaccgg tccagcgtta
     1141 ttcactaatt gtatgatggc gttggattat cgtactggaa aaatgaaatg gttcagccaa
     1201 gcgatcgcac atgatatttt tgatcatgat ctacagatct cacccatact agccaatgca
     1261 actatcagta atgtggaaca tcaaatagtg attggtgcag ggaaaatggg gaatgtgtat
     1321 gcatttgatc gtgaaaccgg tgcgatccta tggacggtcc ccgttgggaa acatgagaat
     1381 gataccctcg atagactttc aggagagact cttgtatatc cagggattct cggtggtgta
     1441 gagacctgca tggcgtactc agatggcatc gtgtttgtac ccgttgtcga cctatccaca
     1501 aagtatcttc caacaggttt aaactactcg agtatcaatt ttgccgaagg gaaaggcgaa
     1561 ctagtcgcaa tcgatgtaaa tctcgggaag atactctggt acaaaacatt tgattcgatc
     1621 aacgtcggtg cagcaaccgt ggtgaacgat gtcgtgttta ccgcaacatt tactggcatg
     1681 atctatgcgt ttcaagccca gaccggacaa caactgtttt cctatcaagc tccagcgggg
     1741 ataaatgcat ggcctgcgat tgctggaaac atgatactgt ggcctactgc agttggtgga
     1801 acaccatctt taatagcact gcatgtgtag attagtaatc tacaataaat gggaaatctg
     1861 agtatattcc cccttttttt cttttttttt aatcgttatt taggtatttt ttaactgtta
     1921 tttggtcata attatatgga tattatctat tgaaaacatt agaggtgata taattatgaa
     1981 aaaaataatt ggaatcttta tttgtatgct gatgaccctc cccgtgttac cactcgctgc
     2041 cgagcagaca aacccgtcaa caatgacaat gatcctaata t
//
LOCUS       DATMXJ010000238         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_51445, whole genome shotgun sequence.
ACCESSION   DATMXJ010000238 DATMXJ010000000
VERSION     DATMXJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51445"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VN377_06300"
     CDS             complement(<1..544)
                     /locus_tag="VN377_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28028.1"
                     /translation="MVFFIIFLLLGTYLPLSSSQQVNKDVFPMNFSEGQFLYSPMWST
                     ATYLRHADGSLNRSWTSSYFPGVAVWWLGNGTILRTIRTGSGGGTGGAGGGVQKVEWD
                     GTVVWDFRYNTNGVLSHHDVKTLPNGNVLLIAWETKTRAEAIAAGRNPSYISNNGFWP
                     DHVIEVQPTGPTSGEIVWEWH"
     gene            763..1896
                     /locus_tag="VN377_06305"
     CDS             763..1896
                     /locus_tag="VN377_06305"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWR28029.1"
                     /translation="MEFDNLKQQALYVKNTPLPGYTFPNFLYLLAQNKFKIDAQYIPR
                     MVYSFTLSTIMLPFYIKERLQFDKRIEQTEITHPPLFIIGHWRSGTTYLQNVLSQDKN
                     LGYLTTFQAYLPGVFLGSEKIFKPLVSSSIPKKRPMDDVLMDADYPQEDQYAIGAFSP
                     YSYYHGWCFPKNMEFYNTSVCMDDVPKETIDEWKAYYLYILKKITLYRHGKQLVLKNQ
                     DNTGKIKILLEMFPDAKFIFLYRNPYDLYFSMMKFMRIAIPRFCIQKPPRIEVVEESM
                     MNLYTKIIQKYIKERVLIPKGNLVEVRYEDFVTQPFQEIKNIYNTLQLEGFRDAEPAF
                     DTYLRTQLIIKKNQYTLNDELKQKIKEKWGFALREFRYENDSI"
     gene            complement(1904..2977)
                     /locus_tag="VN377_06310"
     CDS             complement(1904..2977)
                     /locus_tag="VN377_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWR28030.1"
                     /translation="MRWRNAWIVMRKDLDEFKKQKFVIGSIIAMPIVLGVVLPLAMFV
                     PMVTMVPLDRSWDIDGLLEIGSISDNAIKPWTNQTIEDASSDSTIFRHTILKNASLTH
                     CIIESCVIENTTVTDSTIQNSSLSSSYIMNSTVIRSEGTELDGRFILSISSDLMFTRT
                     KPSEIDQVLPMMFNMILMIFIIVPATLPTIIATYSIVGEKNNRSLEPLLATPTTDGEL
                     LTGKIFSAFIPSMGATLLAFTLGVVLLDIVLIPKVGYPPLPNLTWILSMVLLAPTACL
                     MSVLACVLVSSKVSDVRAAQQLGGFVVMPVVLLMLGVLAGFIFLSPVMIFVFAGLYGC
                     LDLGLFFFVKAIFNRESILTKWS"
     gene            complement(2977..3993)
                     /locus_tag="VN377_06315"
     CDS             complement(2977..3993)
                     /locus_tag="VN377_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWR28031.1"
                     /translation="MEALFTSSTGFIKNKKIRYFSKIVDTDRCRMIICEKLTKTFESV
                     TAIQDFSITIPDGCIFGLLGPNGAGKTTTMRMLSCLIRPTSGKAHIDSYEIGKKTDAQ
                     KIRGMIGLLPEVPGLYETLGAYKNLDYYGQLYGVSKKQREESIRTTLTNLDLWDRRNE
                     PVGGFSKGMKQKIAIARALIHDPKYIFLDEPTASLDPMAAKIVREYIIELKKKGNTIL
                     INTHNLSEAERICDTVALVKNRTVKVGNPKELAKGLFTRTISITMQKIPSTVLKDIES
                     LGFVSQGAVRGNQLILNVKNPEEDNPQVLSWLLKQGLQVQFITEEEHSLEEVYLKLIE
                     EAAR"
     gene            complement(4077..4676)
                     /locus_tag="VN377_06320"
     CDS             complement(4077..4676)
                     /locus_tag="VN377_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR28032.1"
                     /translation="MSGFTIQNGGRLGESVRIDSCYNNFVNNIIEIPNDRIRLSGDYN
                     TFSGNTIEGNYYGLYLYGLNNTINDNTITNNNNGSYISRHEYNTITSNNIIWNNWSGI
                     YLNCSRDNTISGYSISKNIQGIYFVSSTNNTVLKNDFLRNKRHALFENWPNSWNLNYW
                     GRPRILPKLIFGIRIRQNELLVPSLDIDWPPALKPYDLY"
     gene            complement(4746..>4950)
                     /locus_tag="VN377_06325"
     CDS             complement(4746..>4950)
                     /locus_tag="VN377_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28033.1"
                     /translation="PSIAQDREKQSLPKSSGNWLYVGGSGSNNYTMIQDAIDNASDGD
                     TVFVFNGMYVGYVIINKSINLIG"
BASE COUNT         1455 a         1070 c          968 g         1457 t
ORIGIN      
        1 catgccactc ccagacaatc tcaccacttg ttggtcccgt tggctgaact tcaataacat
       61 ggtccggcca aaatccatta ttggaaatgt aactcgggtt ccgcccggct gcaatcgctt
      121 cagcacgagt cttagtttcc caggcgatga gcagcacatt cccatttggt aatgttttca
      181 catcatgatg ggataggact ccgttggtat tataccggaa atcccataca acagtgccat
      241 cccattcaac tttttgaaca ccaccaccag ccccaccggt accgccaccg gacccagttc
      301 gtatagtccg tagaatcgtc ccatttccta accaccatac agccacacca gggaaatagc
      361 tacttgtcca agaacgattc aacgatccat cagcatgacg caaatatgtc gcagtgctcc
      421 acataggtga atataaaaat tgaccttcag aaaaattcat tggaaaaaca tctttattca
      481 cttgctgaga cgatgacaaa ggaagataag tgccaagcaa caaaaaaata atgaaaaaaa
      541 ccatcaatcg aaattcaccg taaactttca ccttagatac tctaaatgac cgcccatact
      601 tcatagtaga tttaataggt gttctctata aatagttttt tcccaatttt gcccctattt
      661 ttgcttttac atcaaatgaa acgacaaaaa aacttgaaaa aaaagcgctc cttctaacat
      721 tcaattactt ttaaatacga gacgaggaaa tatcctcgcc ctatggaatt tgacaattta
      781 aaacaacaag cgctctatgt aaaaaataca ccgctccccg gttatacctt tccaaatttc
      841 ctgtaccttc tcgcccagaa taaattcaag atcgatgcgc aatacatccc acggatggtt
      901 tactcattta cccttagcac gataatgctc cctttttaca tcaaagaacg attgcaattc
      961 gataaacgca tcgaacagac agaaatcaca catcccccgt tgtttatcat cggccattgg
     1021 agaagtggga caacctatct acaaaatgtt ctctcccagg ataaaaatct tggttatttg
     1081 acaacatttc aggcgtatct cccaggagtc tttctaggaa gtgaaaagat cttcaaacct
     1141 cttgtgagtt caagtatccc aaaaaaacgg ccgatggatg acgttctgat ggatgcagac
     1201 tatcctcagg aagatcaata tgcgatcggt gcattctccc cgtattcgta ttatcatgga
     1261 tggtgttttc caaaaaacat ggaattttac aatacttctg tctgcatgga tgatgttccg
     1321 aaagaaacca ttgacgaatg gaaagcatat tatctgtaca ttttaaagaa aatcaccctc
     1381 tatcgacatg gaaaacagtt agttttaaaa aatcaggaca acaccgggaa aataaagata
     1441 cttctggaga tgttccctga tgctaagttt atttttctgt accggaaccc ctatgacctc
     1501 tatttctcca tgatgaaatt tatgaggata gcgatccctc gtttttgcat tcaaaaacca
     1561 ccaaggatcg aagttgtcga agaaagtatg atgaatctgt acaccaaaat tattcaaaag
     1621 tacataaagg aacgagtctt gattcccaaa ggaaacctcg tcgaggttcg ctatgaagac
     1681 ttcgttaccc aaccgtttca ggagataaag aacatatata acacattaca gttggagggg
     1741 tttcgagacg ctgaacctgc gtttgacaca tatcttcgaa ctcaattgat cataaaaaaa
     1801 aatcaatata cgttgaacga tgagctgaaa caaaaaatca aagaaaaatg gggatttgcc
     1861 cttagagaat tccgttatga aaacgacagc atatgatttt tttttagctc catttcgtca
     1921 gaattgattc tcggttgaag attgctttta caaagaagaa aagacctagg tcaaggcacc
     1981 cgtataagcc tgcaaacaca aaaatcatga ctggtgagag aaagataaat cctgcgagca
     2041 ctccaagcat caggaggaca actggcatga cgacaaatcc tccgagttgt tgtgctgccc
     2101 ggacatcact gacttttgag gatacaagga cgcatgcaag gacactcatt aaacaggcgg
     2161 ttggcgcaag aagcaccatc gataagatcc aggtgagatt tggcaatgga ggataaccta
     2221 cttttgggat tagcacaatg tcaagcagaa cgaccccaag ggtgaacgcg agcagtgtcg
     2281 cccccatcga ggggatgaat gcagaaaaga tcttccccgt gagtagttca ccatccgtcg
     2341 tgggtgttgc tagtaatggt tctaaacttc ggttattctt ctcaccaacg atactatacg
     2401 tagcaatgat tgtcggaagg gtcgctggaa cgatgataaa aatcataagg atcatattga
     2461 acatcatcgg aagtacctgg tcgatttccg atggttttgt cctagtaaac ataagatctg
     2521 atgagatgga aaggatgaaa cggccatcca gctctgtccc ttctgaacga atcacggtac
     2581 tattcatgat gtatgaactg gatagtgaac tgttctggat ggtcgaatct gtcactgttg
     2641 tgttttcaat gacacatgat tcgatgatgc aatgagtcag tgatgcgttc ttcagtattg
     2701 tatgcctgaa aatcgttgag tcagacgaag catcttcaat ggtttggttt gtccatggct
     2761 tgattgcatt gtcagatatg ctaccaatct caaggagtcc gtcgatatcc catgaccggt
     2821 caagcggaac catggtcacc atgggaacaa acatcgcaag tggcaagaca acccctaaga
     2881 caatgggcat ggcgatgatg gatcctatga cgaatttctg tttcttgaat tcatctaaat
     2941 ccttccgcat cactatccag gcgtttcgcc agcgcatcac cgagcagcct cctcgatgag
     3001 tttaaggtac acttcctcta atgaatgttc ctcctcggtg ataaattgaa cctggagtcc
     3061 ttgtttgagt aaccaggaga gcacctgtgg gttatcctct tctgggttct tcacattgag
     3121 aatcagctga tttcctctca ccgccccttg ggagacaaag cctaacgatt ctatgtcttt
     3181 cagtactgtc gaggggattt tttgcatcgt gatactgatt gttcgagtga acaatccttt
     3241 tgcaagttct ttaggattcc caaccttcac agttctattt ttcactaaag cgaccgtgtc
     3301 gcagatccgt tctgcttctg atagattgtg tgtgtttatc agaatggtgt ttcctttttt
     3361 tttcagttcg atgatgtact ctctgactat ttttgctgcc attgggtcaa gactcgcggt
     3421 tggttcatca aggaaaatat attttggatc atggatcaat gcacgtgcaa tcgctatctt
     3481 ctgtttcatg cctttggaaa atccaccaac aggttcgttt cgtcgatccc ataaatctag
     3541 gtttgttaat gtcgtgcgaa tcgactcctc tctttgtttt tttgatacac catagagctg
     3601 tccataataa tcgaggttct tgtacgcccc cagagtttcg tacaatcctg ggacctctgg
     3661 gagtaacccg atcatcccac ggattttctg cgcgtctgtt tttttcccaa tctcatagga
     3721 atcgatgtgt gcctttccag acgttggtcg gatgagacag gagagcatcc gcatggtagt
     3781 ggtttttccc gcgccgtttg gcccaagaag tccgaagatg cagccatcag gtattgttat
     3841 ggaaaaatct tgtattgcag tgactgattc aaatgttttc gttaactttt cacagataat
     3901 catacgacac cggtccgtat caacgatctt actaaaatac cgtattttct tattcttaat
     3961 aaatccagta gatgaggtga ataatgcttc catccaaagt ctttagtctt aatttatttt
     4021 tataaaaacg tgtttttaac aattgatcac agatatctct caaaaaattc tcacatttaa
     4081 taaagatcat agggttttag tgcgggaggc cagtcaatat cgagtgatgg tactagcaat
     4141 tcattctgtc taattcttat gccaaaaatg agtttaggga gtatccgtgg tctaccccaa
     4201 taatttaggt tccaactgtt cggccagttt tcgaaaagag catgtcgttt gtttctcaga
     4261 aaatcgtttt taaggacggt attgttggtt gatgagacga aatagatacc ttgtatattt
     4321 tttgaaatgc tgtaacctga gatggtgttg tcacgggaac aattcagata gataccggac
     4381 caattattcc agataatatt attactagtg atagtattgt attcgtgtct tgagatgtag
     4441 ctaccgttat tgttgtttgt gatagtgttg tcatttatag tgttgttcaa accgtaaagg
     4501 tataaaccat aatagtttcc ttcgatggtg ttacccgaga aggtgttata atcaccagag
     4561 agccgtattc tatcattggg gatttcgatg atgttattga caaagttgtt gtagcatgaa
     4621 tctattctaa cactctcacc taaacgcccc ccgttttgaa tagtaaaccc actcatattc
     4681 acccaatctg aaataattga aatggtatac gcaaaaaacc cattgataat cgttgtattc
     4741 ttatcctacc caatgagatt aattgattta ttgatgatga catatcctac atacattcca
     4801 ttgaagacaa acaccgtatc cccatcactt gcattgtcga tcgcatcctg aatcatcgta
     4861 tagttattcg aacctgagcc gccaacatac aaccaatttc cactcgattt tggtaatgat
     4921 tgtttctctc tatcctgtgc tatactagga
//
LOCUS       DATMXJ010000239         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_67764, whole genome shotgun sequence.
ACCESSION   DATMXJ010000239 DATMXJ010000000
VERSION     DATMXJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67764"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VN377_06330"
     CDS             <1..661
                     /locus_tag="VN377_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR28034.1"
                     /translation="SMSMDIFTIPSQDYDSNIYVIIGETPTIIDTGTGFHSHMIIEAI
                     KKKINLHKIRQILLTHEHYDHVGGVQDILRATDGSARVFAHRDIVSKLKEGKSTFAEM
                     LGGVMPNIEVDVPLTENALITVGDESFQVLVTPGHSIGSLCFYSTKNEILFSGDTIFA
                     NGGFGRYDFPGGDFRSLLHSIERLSTLNIKNLYPGHGPIIETCGKDHVLAALSNIRSL
                     I"
     gene            964..2238
                     /locus_tag="VN377_06335"
     CDS             964..2238
                     /locus_tag="VN377_06335"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HWR28035.1"
                     /translation="MSLSEKTSVVSGFYKLPIEKRREFVAQFVDLNEDEVKSFSSCLD
                     IATADRMIENVLGIFELPLGLAVNFLINNKDYIIPMVTEESSVVAAASNAAKIARVKG
                     GFSTTCTDPLMIGQLQLLHVKDVSIAAQRIKQHKKQLLAVANAQDKILVNFGGGAKDI
                     DVRILESPLGQMIIIHLIVDVRDAMGANAVNTMCEALAPMLEEITGGKVRLKILSNLA
                     DKRLVKATATFDKATMGGDHVVDAFLESYVLASIDPYRAATHNKGIMNGIDALIIATG
                     NDFRAIEAGAHAYAARGGRYTSLTRYHKNENGDLVGEIELPMAVGVVGGAANMHPKAK
                     LCRKILGVKTARELAEVVASLGLAQNFAALFALSTVGIQKGHMSLHAKNIAVMAGAQG
                     NEIEKLAEQLVHDGKVKLDYAKELLEKIRRIS"
BASE COUNT          670 a          419 c          533 g          632 t
ORIGIN      
        1 ctccatgtct atggatattt ttactatacc tagtcaggac tatgattcta acatctatgt
       61 tattattggg gagactccga ctattattga tacgggtaca gggtttcatt ctcatatgat
      121 tattgaagcc attaaaaaaa aaattaattt acataaaata cgacagattc ttcttaccca
      181 tgagcattac gatcatgttg gaggggtgca ggatattctt cgtgcgacgg atggatctgc
      241 acgggttttt gctcatagag atattgtgtc aaaactcaaa gaagggaaaa gtacctttgc
      301 tgagatgctt ggtggggtaa tgccaaacat agaggtcgat gtcccattga ccgagaatgc
      361 gctaataacc gttggagatg aatcttttca ggttctcgtc acgccaggtc atagcattgg
      421 gagtctttgt ttttatagca cgaaaaatga gatccttttc tcaggagata cgatatttgc
      481 gaatggtggt tttggtcgat acgattttcc cgggggggat tttcgttcgc tgctccattc
      541 aatagagcga ttgtcgacat tgaacatcaa aaatctttat cctggtcatg gcccaatcat
      601 agaaacatgt gggaaagacc acgtgttagc agcgctttcc aacattcggt cgttgatcta
      661 aatgttctat gttcgagata ttcttaataa aattaaatgg acgaaggatt taaaaaaggt
      721 aacaatatgg tatatccatc gaggggcgtt gcataatacg aagacgattt ctggtgatga
      781 aattatatct attggtagat catttcttga gacatcaact gcaacgatac cgtatcatcg
      841 aatcataaaa gttatctacg gagatgaggt tctattcaat cggtgggaca tccgttctca
      901 gtataaacag ttataaaaat gtgaactgtt ttcctacgta gtacataagg aggtagaaaa
      961 gacatgtcgc tatctgaaaa gacgtcggtt gtgtcaggtt tttataagct tccaattgag
     1021 aaacgaaggg aatttgtggc ccagttcgtg gacttaaatg aagacgaagt gaagagtttt
     1081 tcttcatgtc tcgatattgc aaccgcggat cgcatgattg aaaatgttct aggtatcttt
     1141 gagttgcccc ttgggttagc agtaaatttt ctgattaata acaaagatta tattattccg
     1201 atggtgactg aagaatccag tgttgttgca gccgcgagta acgcagcaaa aatcgctcgt
     1261 gtgaagggcg gattttctac tacatgtacc gaccctctga tgatcggtca acttcaatta
     1321 ttgcacgtca aggatgtttc catagcagcc caaagaatca agcaacataa aaagcagtta
     1381 cttgctgtgg cgaacgctca ggataaaatc cttgttaatt tcggtggggg cgcaaaggac
     1441 atcgatgtaa gaatcctcga gagtcctctg ggtcaaatga tcataatcca ccttattgtt
     1501 gatgtccgtg atgcaatggg agcaaacgcg gtgaacacca tgtgcgaagc gctcgctccg
     1561 atgttggaag aaattactgg tggcaaagtg cggctgaaaa tactatcaaa tcttgcggat
     1621 aaaagactcg tcaaagcaac agcgaccttc gataaggcaa caatgggggg agatcatgtg
     1681 gtagatgctt ttcttgaatc atatgtcctt gcatctatcg atccttatcg ggctgcaacc
     1741 cataacaaag gcattatgaa cggtatcgat gcattgatca ttgcgacagg gaacgatttt
     1801 cgtgccatag aagctggcgc tcatgcgtac gcagcacgtg gtggtcgata cacctctctt
     1861 acacgatatc ataagaacga aaatggagac cttgttggtg aaattgaatt accaatggcc
     1921 gtgggtgtgg tcggaggtgc agctaatatg catccgaagg caaaactctg tagaaaaata
     1981 ctcggagtta agacggcacg agaactggct gaggtcgtcg ccagtcttgg tcttgctcaa
     2041 aactttgctg cactattcgc tctctcaacc gttggtatcc agaaggggca tatgtcgtta
     2101 catgcaaaaa acatcgctgt gatggcgggt gcacaaggaa acgaaatcga aaaactcgct
     2161 gagcaacttg tccacgatgg aaaagtaaaa cttgattatg caaaagagtt gttagaaaaa
     2221 ataaggagaa ttagttagac aattcctgca gcta
//
LOCUS       DATMXJ010000240         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_24817, whole genome shotgun sequence.
ACCESSION   DATMXJ010000240 DATMXJ010000000
VERSION     DATMXJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24817"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VN377_06340"
     CDS             <1..455
                     /locus_tag="VN377_06340"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWR28036.1"
                     /translation="EDVCKNLKARHDSGKKFSIVVASEGAKPSDTDHFETLSEEKDEF
                     GHARLGGIGQRLAKEIEKRLGVETRFTVLGHVQRGGSPTAYDRVLATRYGVVAVELVK
                     EGHFGKMVALRGYKIVPVDLNESVTKLRTVDMDLYNIATTFFAKPHNE"
     gene            589..885
                     /locus_tag="VN377_06345"
     CDS             589..885
                     /locus_tag="VN377_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28037.1"
                     /translation="MVWNKKFLMIIGLGGGIMLFFIGMAAYIILGPSTDTYLFPKQVS
                     SVIKLTGMGIVCISMVVGGFFVEKMEKDTKLLLLLFGVILLLLNIFLFSSSRYY"
     gene            911..1333
                     /locus_tag="VN377_06350"
     CDS             911..1333
                     /locus_tag="VN377_06350"
                     /inference="COORDINATES: protein motif:HMM:NF045529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Heimdall-CTERM domain-containing surface
                     protein"
                     /protein_id="HWR28038.1"
                     /translation="MKSLITLGLIVGIAFFFIGAMISNVFPSSGTDLLSYKISSSIKL
                     LGIGTVICSMVIGGIYVEEIDKNLKLLLLLLGLVLLVVYTIGSQSLEWYIPTSQTPSG
                     IESYEHRPTGYGVPGFELILGLGALAITLLILKKRHYR"
     gene            complement(1774..2694)
                     /gene="rnz"
                     /locus_tag="VN377_06355"
     CDS             complement(1774..2694)
                     /gene="rnz"
                     /locus_tag="VN377_06355"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HWR28039.1"
                     /translation="MTQLRIIFLGTGGSWPTVKRNVSSIAVKRGSEIILFDCGEGTQR
                     QFQQSNLSYMQISKIFITHFHGDHFLGIPGLIQTMQLNDRDKPLHIYGPKGMVELTNQ
                     LLTLGYFRPSYRVIAHEVTDGEEIKFDGYSISALKVSHNVPALAYSVKEYQRAGKFNK
                     SKALKMGIPEGPLFSKLQKGQTIILPNGEKVTPDLILGPSRKGRKIVISGDTKPSNQI
                     IGFSEDADVLIHEATFDSRLQEIAKEYGHTTAVQAAEIAKKAGVEKLFLVHISPRYLD
                     YHVLEDEARSVFPHSYAPKDFQEVEVKLKK"
BASE COUNT          788 a          557 c          583 g          864 t
ORIGIN      
        1 ttgaggatgt gtgtaaaaac ctaaaagcgc gacatgacag cggaaagaaa ttcagcattg
       61 ttgtcgcctc agaaggagca aaaccaagtg ataccgatca ttttgagaca ttatctgaag
      121 aaaaagatga gtttggccat gcgcgtctcg gtggtatcgg gcaacgcctt gccaaggaaa
      181 tcgagaaacg acttggtgtg gagacacggt tcaccgtcct tggacatgtg caacggggtg
      241 gatccccgac ggcatacgac cgggttcttg cgacacgata tggtgttgtt gccgttgaac
      301 tggtaaagga aggtcatttt ggaaaaatgg tcgccctacg agggtataaa atcgtgcctg
      361 tcgatctgaa cgaatctgtc acaaaactca ggacagttga catggatctc tataatattg
      421 cgacaacctt ctttgcgaaa cctcataatg aataaatcag tgatcgattc gatttgttat
      481 tataaaaatg ttttaaaatt acgaatcaat gatatttcaa ttaatattca taattatgaa
      541 ccaataatta tataggggat aaagaggttc catctctgga gtgaaagaat ggtatggaat
      601 aaaaaatttc ttatgattat aggcttgggt ggtggtatta tgctcttttt tatcggcatg
      661 gcggcctaca tcatcctggg acctagcaca gatacctatc tcttcccaaa acaggtctca
      721 tcggtgataa aactcacagg tatgggaatc gtttgtatct caatggttgt cggaggtttc
      781 ttcgtggaga aaatggaaaa agatacaaaa ctcttgttgc ttttattcgg ggtgattctg
      841 ttgctgttaa acatcttcct cttctcatca tctcggtatt attaggagat gagaaacaat
      901 gaagatgaac atgaaaagtt taatcacact tggacttatc gttggaatcg cattcttttt
      961 tatcggtgcg atgatcagta acgtcttccc aagcagcgga actgatcttt tatcatataa
     1021 aatctctagt tcaatcaaat tacttggtat tggaactgtc atatgctcga tggttatcgg
     1081 tggaatctac gttgaggaaa ttgataaaaa cttaaagctt ttgcttctct tgctcggttt
     1141 agtcctcttg gtcgtctaca ccataggatc acaatccctt gaatggtaca taccgacatc
     1201 acaaacacct tcgggaattg aatcctatga acatcgaccc acagggtatg gtgtacccgg
     1261 ttttgagctg atacttggct taggagcact tgcaatcacc ttgctgattc tgaagaaaag
     1321 acactatagg taagcaacca atggaaacag aaaacacgcc ctctgctccg acaagtattg
     1381 ttttgatttc aatattctgg atacttatag ggacgtttat cctcgcaata acctccaggt
     1441 acctctccgc catatacagt tacaacatac ttctgtttgg attaatccct ttcatgatag
     1501 ctataggttt aatcatggta ggttggggac tccttacatt cagaaaatgg gcatattata
     1561 tcgccttaat tttttcacta cttggcacaa tctttttgtt aatctttaat acctttttca
     1621 tcggttatcg attcctgtct gattggtatt acattgatat tgaaacgatt ctatggacat
     1681 cattaccgat aatatttttg ttaatgtttg tttttatgtt ctggtacctc atcaaaaata
     1741 agaattatta tgaaaaagaa caatagagtg tcactatttc tttagcttta cctcaacttc
     1801 ctgaaaatcc tttggggcgt atgaatgggg aaacacactg cgagcttcat cctcaagtac
     1861 atgataatca agatagcgcg ggctgatatg cactaaaaac agtttttcaa ctcctgcttt
     1921 tttcgctatc tctgctgcct ggacagcggt cgtgtgtcca tattctttgg caatttcttg
     1981 aagtcgtgaa tcaaaggtag cctcatgaat aaggacatct gcatcctctg aaaaaccgat
     2041 gatttgattc gatggttttg tgtcacctga aatcacgatc ttcctacctt tccttgaagg
     2101 ccccaaaatt aaatctggcg tgactttctc cccattaggg agtatgattg tttgcccttt
     2161 ttgtaattta ctaaataaag gtccctcagg gatccccatt tttaaagcct tggacttatt
     2221 aaacttccca gcacgctgat attcctttac gctatatgcg agcgctggga cattatggct
     2281 gacttttaac gccgatatgc tgtagccatc gaatttgatc tcctcaccgt cagtgacctc
     2341 atgagctata acacgatagg atggacgaaa atatcccaat gtgagaagct ggttggtgag
     2401 ttccaccatt cccttcggac cataaatatg aagaggtttg tctcgatcgt tcaactgcat
     2461 cgtttggatc agaccaggta tcccaagaaa atggtcacca tgaaaatggg tgatgaagat
     2521 ttttgatatc tgcatatagc ttagattgga ttgctggaac tgtcgttggg ttccttcccc
     2581 gcaatcaaat aaaatgattt cactgcctct tttcacagcg atggacgaaa cgtttcgttt
     2641 cacggtcggc caacttccac cggttcctag aaagatgatt cgtaattggg tcatgtacgg
     2701 gacggatact ccctatgggg gtaaaagcgt tttggtcttg attcccgttt caaagaagaa
     2761 aaaaagagga agaaaacccc cttggttgta tg
//
LOCUS       DATMXJ010000241         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_20659, whole genome shotgun sequence.
ACCESSION   DATMXJ010000241 DATMXJ010000000
VERSION     DATMXJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20659"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..959
                     /locus_tag="VN377_06360"
     CDS             675..959
                     /locus_tag="VN377_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWR28040.1"
                     /translation="MNFQAVGRTKYHLHVLGEVAPWYSLADVMQICNSITAKQLFKKS
                     PDLEEELWGGHFWSEGGYIDTAGASHDVDTMGDYINKQGIFKLQNEAVSM"
     gene            complement(1608..2867)
                     /locus_tag="VN377_06365"
     CDS             complement(1608..2867)
                     /locus_tag="VN377_06365"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR28041.1"
                     /translation="MEKKYISKLLVIGVLFLFLGASATLGSANRAWADNFNSYANDQY
                     LEDGSPGDGGWAGWGIPPDLTAGAYVRDDHYRSSPYSVEIAVTSDLVQQFEGYTTGVW
                     EFIAWQYIPTDFTGDTYFIMLSAYDGSGVEPGCTWTVQLKFSGGLIENQWTAETMPYV
                     AGAWKKISCLIDLDTDWLTIKYNGQLLAQHAYTDGIGGGTGPATLNIAAVDLFSDTGS
                     IVYYDDLSLGAPTAELIAYCGGPYFSEIGEDIIFDGSAEGGAAPYTWAWDFGDGNTST
                     EQNPTHAYDAAGVYNVSLTVTDAAEETATATTTATITAPSPVLEFGAITGGFGIKSSV
                     KNTGDGAATNVDWTIALDGKLVFLGKSTPGTIATLAPAGDEAIKSSFILGFGKTNIVV
                     SATCDEGVTAEATASAFVFGPFVLGVK"
BASE COUNT          812 a          617 c          613 g          929 t
ORIGIN      
        1 gtctagatgt tactacagga ttggttcttg gtcttggatc tgtcgatatt acagtgactg
       61 ctaatgatgt tcaaaaaaca gcgaccgctt tcctcttagg accgttcgtt ctgaacatca
      121 ggtgatctct cgcacaggtc atttcatcat tcattgcaga aagattcaga attgataaac
      181 gtataagagt aaaaatgata gggccactat gagaacagtg gttttatcac aatctttttc
      241 gtagcgatta ttacaagatg ttacccattt catgatgagg cggtcaactt aagatacctc
      301 ccgaagaaga ttatacaaaa ctatccaagc aatagaccaa tgactaatat tctgtaacgt
      361 gctgtgtggg aaatacctac agacgcgttt aacccgagtt ttgtactgga tgtaccactg
      421 ttcaatatga tttcacatca tctatgagaa tgaaaggttt attcctgtcg gagttacata
      481 ccccggagat cttccccggt aggttcacac ttctttgatg atgattgctt gatggaagta
      541 aaatatgctt ctgattgtag ttatcgtata cgatacctta tggtatttgt cctcaaatac
      601 agaaaaatct tattagtact gagatttttg aggccatcaa agaaatcctt ttagggattt
      661 cagaaagata ttaactgaat tttcaagctg ttggtcgtac aaaatatcat cttcatgttc
      721 tcggtgaagt agctccatgg tactctctgg ctgatgtaat gcagatttgt aatagcataa
      781 cagcaaaaca acttttcaag aagtctcctg atcttgaaga agaattatgg ggcgggcatt
      841 tttggtccga aggtggatat attgatactg ctggggctag tcatgatgta gacactatgg
      901 gggattatat caataaacag ggtatcttta agctgcaaaa tgaagctgta tcaatgtgaa
      961 tcgccgtaat accctggagt tccactccgg ttggtataag taatgcgttc gttttacttt
     1021 cactatagtt caaagacctt cttcagaaaa aatatcgcgt taaatgacgt attctcacca
     1081 aagcataaac cgcaagaatc aaatggtcct caatgtcgat ggcattccag atatagatct
     1141 accgtccttc ccacatcaca atcgttacat cgatagcaat aacgagtcac tatctccggg
     1201 tggctgaaga agacgacgcc cttcttatat cattttagca atacctcgtg aaagaatgat
     1261 tccagattgc ggatgatgac atgggtcttt ttatatgaaa gaatagcatg atacacaaga
     1321 agtgctatca cttttatctc gtggtgaacc ctctgcctta caaagatttt tccatcttct
     1381 tacaattcca ctatttgata gaacataccc atcctttcct ttgttcttac actggatgga
     1441 cttgctcaat taaaagactg tttccttaag ttgacagtct tcgttcagca ttgtaaatgg
     1501 tgcttctcag gtttaaatga tttgatagaa ataaattttt attcctctta aaattgagaa
     1561 taaattcaca aaattaaatt aaaaaaaaga aaaaaagggg ggagggatta tttcactcca
     1621 aggacgaatg gtccgaagac aaacgcactt gcggttgcct ctgcggttac gccttcatcg
     1681 caggttgctg atacgacgat gtttgttttg ccgaagccaa ggatgaaact tgattttatg
     1741 gcttcatcac cagcaggtgc gagggttgcg atggttcccg gggttgattt cccaaggaag
     1801 accaattttc cgtcaagagc aatcgtccag tcaacgtttg ttgctgctcc gtctccggtg
     1861 tttttcactg aggatttgat accgaatcca ccggtaattg ccccaaattc tagcacgggt
     1921 gaaggtgcgg tgattgttgc ggttgttgtg gcagttgctg tttcttcagc ggcatcggtg
     1981 actgtgaggg agacattgta gacgcctgct gcgtcatagg cgtgggtcgg gttttgttct
     2041 gttgaggtgt tcccgtcgcc aaagtcccat gcccaggtat agggtgctgc tcctccttcg
     2101 gcagaaccat cgaatatgat atcttcacca atttcactaa agtatggtcc accacagtaa
     2161 gcgattaatt ctgctgttgg tgccccgaga gataggtcgt cataatacac aatagatccc
     2221 gtgtctgaaa agagatcgac tgcggcgatg tttagtgttg ctggccctgt accaccgcca
     2281 ataccgtccg tgtatgcgtg ctgagcgaga agttgtccat tatatttaat tgttagccag
     2341 tcagtgtcta aatcgattaa acaactaatc tttttccatg cgccagcgac atacggcatg
     2401 gtttctgcag tccactggtt ttcaattaac ccgccgctaa attttaattg tacagtccat
     2461 gtacatccag gctccactcc agaaccatcg tatgcactga gcatgatgaa atatgtatct
     2521 cctgtgaaat cggttgggat gtattgccag gcgataaatt cccatactcc tgttgtgtat
     2581 ccttcaaatt gctgaacaag atctgaagtg acagcgatct cgactgagta ggggctgcta
     2641 cggtaatgat catctcttac atatgcaccg gcagttaagt ccgggggaat accccatcct
     2701 gcccatcctc catctcctgg acttccatct tctaaatatt gatcgttggc gtacgaatta
     2761 aaattatccg cccatgctcg attcgcactc cctagtgtgg cacttgctcc aaggaatagg
     2821 aataatactc ctattaccaa tagtttgctt atatatttct tttccatttt tatattgcct
     2881 ccttatattc tgtttatact tccatattct taatagtgct tcttcgattt aaatgttttg
     2941 atgcaaataa attttatttt tttcttaaaa t
//
LOCUS       DATMXJ010000242         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_15417, whole genome shotgun sequence.
ACCESSION   DATMXJ010000242 DATMXJ010000000
VERSION     DATMXJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15417"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..401
                     /locus_tag="VN377_06370"
     CDS             87..401
                     /locus_tag="VN377_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28042.1"
                     /translation="MRKTLIGSSLLVVLLLLMLPAVTAEQVKIAQSATTTNLNTIEAT
                     YMEAIRQKYKDNPSPQIILITLAILLLKLLRWGLVIIGGILILAILGILRKPNNNTSA
                     VL"
     gene            678..>3448
                     /gene="uvrA"
                     /locus_tag="VN377_06375"
     CDS             678..>3448
                     /gene="uvrA"
                     /locus_tag="VN377_06375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWR28043.1"
                     /translation="MKKIIIKGAREHNLKNITLELPRDKLIVITGISGSGKSTLAFDT
                     LYAEGQRRYVESLSAYARQFLGLMNKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTTT
                     EIYDYLRLLFARIGTPYCPTHNIKIESQSPQKISTRIAAELIGTITILSPIIRQKKGT
                     YEQLFKDLNKEGYTRIRVNKTIHRTDEKIPLERYKKHDIDIVIDRVETSDTARLAEAC
                     EQALQKSDGLLIALDATSHEHIYSSTMACPICGTVFEELQPRMFSFNSPFGACEACHG
                     LGIKMEFDQDLIIPNKELSIADGAIALYKTIRDSWRNNYLGAVAKHYGFNIFTPIKNL
                     NEQQYDAIMYGSSEPIRFNFVMKNGDAHYSHIGTWEGLIPQSERLYKQTESEYRRQDM
                     ERFMRISPCPTCHGKRLKQKILSIKIAQKSIIDLTDMSIKQSLHFFETLHLTKKQEEI
                     AHLILKEINARLKFLDNVGLNYLTLSRNAGTLSGGEAQRIRLATQIGSNLMGVLYILD
                     EPSVGLHQRDNKKLIQTLQRLRDLGNTLIVVEHDEETIRHADYVIDMGPGAGIHGGKI
                     IAKGTPHQIEHNPRSLTGQYLSGKKAILVPETRRKTTKHIHIKGCKENNLKNIDVHIP
                     IGVFTVITGVSGSGKSTLIYEILYKGLMKKLHESRDPPGKHDEIAFDSEIDKVIVIDQ
                     SPIGKTPRSNPATYTKVFDEIRTIFTLTPEAKLRGYRAGRFSFNVPGGRCETCQGDGL
                     IKIEMNFLPDIYVECEECKGKRYNKETLEVSYKGKSIADVLAMSVEEALDLFENIPYI
                     RKKLETLSRVGLNYIKLGQSSTTLSGGEAQRIKLTRELSKKSTGRTLYLLDEPTTGLH
                     FHDTKKLIDVLNSLVNMGNTIVVIEHNMEVIKCADHIIDLGPEGGDLGGEIVAVGTPE
                     EVAGDKQSYT"
BASE COUNT         1094 a          878 c          712 g          764 t
ORIGIN      
        1 tatttttatc tgttttgtta acttatattg ttacgataac atttaaatgc tagtggcgta
       61 taacaaaaaa ctggaggacg tataaaatga gaaaaaccct aattggcagt agtctgttgg
      121 ttgtattgct tttactgatg ctaccagcag tgaccgcaga gcaggttaaa attgcacaat
      181 ccgcaacgac cacgaatctt aacactattg aggccaccta tatggaggcg attcgacaaa
      241 aatataaaga caatccatcc ccgcaaatca ttctcatcac cttagctatc cttttgttaa
      301 agctcttgcg atggggactt gtcattatcg gtgggatact cattctggcg attcttggta
      361 ttctccggaa accgaataac aacacaagtg cggtcctgta gtctaaccat cattgttcgt
      421 tttcttcgaa acattggtta tttggaaggc tgttgagtgc tatccttttt tctttcttta
      481 ctctttgatt ttttatcatt aaagtataaa aaccaataga gtatggttct gaatagatac
      541 tttcgatgac cctttagaaa accttgaata tgggagagtg cttgccggag aactaaccga
      601 tataacattc ggccacattg tatcaataaa atgtatatat ctgtatatat tatcactatt
      661 ttcaaagggg atttccagtg aaaaaaatca tcatcaaagg agcacgagaa cacaacctaa
      721 aaaacatcac cctagagctt cccagggaca aactcatcgt catcacaggg atctcaggct
      781 cagggaaatc caccctcgca tttgacacgc tctatgcaga aggacaacga cgctacgtcg
      841 aatcactctc tgcctacgca cgccagttct taggcctgat gaacaaacca gatgtcgaca
      901 gcatcgaagg attatcccct gcgatttcca tcgaacaaaa aacaactagc aagaacccac
      961 gtagcactgt cgggaccacc acagaaatct acgattacct ccgcttactc ttcgcccgca
     1021 tcggcacccc gtactgcccg acgcacaaca taaaaatcga gtcgcaatca ccacagaaaa
     1081 tctcaacgag aatcgcagca gaactgatag ggacaatcac cattctctca ccaataatcc
     1141 gtcagaagaa aggcacctat gaacaactct tcaaagattt gaataaagaa ggatacacca
     1201 ggatccgcgt taacaaaacc atccatcgaa ctgatgagaa aataccctta gagcgttaca
     1261 agaaacatga tatcgacatt gtgatcgacc gcgtcgaaac atccgacact gctcgactgg
     1321 cagaagcctg tgagcaagcc ttacagaaat ccgacggcct gctcatcgcg ttagatgcaa
     1381 ccagccatga gcatatctac tcatcgacca tggcctgtcc tatttgcggc acggtcttcg
     1441 aagagctcca gccgcgcatg ttctccttta acagcccctt cggcgcctgt gaagcatgcc
     1501 acggacttgg catcaaaatg gaatttgacc aagaccttat catcccaaac aaagaactca
     1561 gcatcgccga cggtgcgatc gccttgtaca aaaccatcag ggacagctgg aggaacaact
     1621 atctcggagc ggttgcaaaa cattacggat ttaatatctt cacccccata aaaaacctca
     1681 acgaacagca atacgatgcg atcatgtacg gatcgtcaga gccaatccgt tttaacttcg
     1741 tcatgaaaaa cggtgacgca cactactccc atatcgggac atgggaagga ctcatccctc
     1801 aatcagaacg actctacaaa cagaccgaat cggaataccg cagacaagac atggagcgat
     1861 tcatgcgcat cagcccatgc cccacctgcc atgggaaacg attaaaacaa aaaatactct
     1921 ccatcaaaat cgctcagaaa tccatcatcg atctcaccga tatgtcaatc aagcagtcgc
     1981 tacacttctt tgaaacgctc cacctgacaa aaaaacaaga ggagatcgcc catctgatcc
     2041 taaaagagat caatgcacga ttaaaattct tagacaacgt cgggttgaat tatctcacac
     2101 tctcacgaaa cgcaggtaca ctctccggcg gggaagccca acggatacgg ttagcgaccc
     2161 agatcggatc gaacctcatg ggagtcctct acattcttga tgaaccatcg gtcgggttgc
     2221 atcagagaga taataaaaaa cttatacaga cattgcaacg cttacgcgac ctgggaaaca
     2281 ccctgatcgt cgttgaacat gacgaagaaa ccatacgtca tgccgactat gtcatcgaca
     2341 tgggccctgg tgctggaatc catgggggga agatcatcgc caaagggacg ccacatcaga
     2401 tcgaacacaa cccccgctcg cttaccggac agtatctctc tggaaaaaag gcgatactag
     2461 tcccagagac cagacgaaag accacgaaac atattcatat caaaggatgc aaagaaaaca
     2521 acctcaaaaa catcgatgta cacatcccca tcggagtctt caccgtcata accggggttt
     2581 ccggaagcgg gaaatcaaca ctgatttatg aaattctcta caaaggtctg atgaaaaaac
     2641 tccatgaatc acgagatccg ccagggaaac atgacgagat agcgttcgat tcagagattg
     2701 ataaggtcat cgtcatcgac caaagcccga taggcaaaac cccacgcagc aacccagcga
     2761 catacacaaa agtattcgat gagatcagaa ccatcttcac cctgacccct gaggcgaaac
     2821 tccggggata tcgagcaggt cgcttctctt tcaatgtgcc tggcggacga tgcgaaacat
     2881 gccaaggcga cggtcttatc aagatagaga tgaacttcct cccggacatc tacgtggaat
     2941 gtgaggagtg caaaggaaaa cgttacaaca aagaaaccct tgaggtgtcg tataaaggaa
     3001 aatcgatagc ggatgtcctt gcgatgagtg tcgaggaggc ccttgacctc ttcgaaaaca
     3061 tcccttatat ccgcaagaaa cttgaaacac tctcacgagt aggtttaaac tacattaaac
     3121 tcggtcaaag ctccacgact ctttcaggtg gggaggcgca gcggatcaaa ttgacgcggg
     3181 aattatcaaa gaaaagcaca ggacgaactc tctaccttct tgatgagccg accaccgggc
     3241 ttcatttcca tgataccaag aaactcatcg atgtgctcaa cagcctagtg aacatgggca
     3301 acaccatcgt cgtcatcgaa cataacatgg aagtgataaa atgcgcagat cacatcatcg
     3361 acctcggtcc agaaggcggt gatctcggcg gagagatcgt tgcggttggc acaccagaag
     3421 aggtagctgg tgacaagcag agttatac
//
LOCUS       DATMXJ010000243         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36343, whole genome shotgun sequence.
ACCESSION   DATMXJ010000243 DATMXJ010000000
VERSION     DATMXJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36343"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /gene="truD"
                     /locus_tag="VN377_06380"
     CDS             complement(<1..1222)
                     /gene="truD"
                     /locus_tag="VN377_06380"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HWR28044.1"
                     /translation="MNAPDFEHEIGIDTYCTDHPGVDGKLRVRIDDFHVTELFLYPPK
                     KDQGHFTIAEVTSRNWENHTLVQEIAHRLHLSQRRISFAGTKDKRAWTTQLMSFEHVP
                     PEQLSSLSIKDVTIENIFQSDVPVRIGELLGNHFDITIRNIPEEIVPEQIISLISPFQ
                     KWSGFPNFYGIQRFGVIRPITHLVGKYIIQGDFEKAAMTYVGNPMSGENETTFALRTE
                     LEKTRDYTKAFHSYPETLNFEKAILNKLIQDPQDFIGAFKELPKNLLMMFVNAYESLL
                     FNKILSERIKRNLPLHQAVIGDIIYPMRKNVIVNEAIPVTESNIDKVNAQLSKKKAVV
                     TGLLVGYDSMYADGEMGKIEHAIIDSQEIAPRDFIIPDIPFLSSSGSRRPLLALLPSL
                     EWTLHTDEYASGRQA"
     gene            complement(1238..1504)
                     /locus_tag="VN377_06385"
     CDS             complement(1238..1504)
                     /locus_tag="VN377_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HWR28045.1"
                     /translation="MDLKTIRKTKTELELEITGEDETILHPITHILLQNDDVEYAACM
                     SDNPLAVKKRLFIRVKKGSPEDVLKKAVKYLEDEIKLFGKNFEK"
     gene            complement(1530..2099)
                     /locus_tag="VN377_06390"
     CDS             complement(1530..2099)
                     /locus_tag="VN377_06390"
                     /inference="COORDINATES: protein motif:HMM:NF034126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HWR28046.1"
                     /translation="MKHKLVMPGDQLSTSEELLPGDGTFEENGIIRAARIGTYVVDEK
                     HRTAEVKPASSTPVLLKKGDIVIARVTNAKPTMIIVDVVHVAGKNRSISGDTDATVHV
                     KEIAQGFIKDASTEYKSEDYIRAKVIQVKPSVQLETKERNFGAIKALCSKCRHPLIKK
                     SHGLECENCGNKEHRRIAEDYGNLDIQHL"
     gene            2262..2780
                     /locus_tag="VN377_06395"
     CDS             2262..2780
                     /locus_tag="VN377_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWR28047.1"
                     /translation="MSRKPARMIRQVRGQATTRREYMGGVPASRLTQFVIGNKTAKFP
                     VQLSLVTKEKCQLRHNALESARISANKSLEKYIGSANYRLHIRVYPHIVIRENKQATG
                     AGADRVSQGMRRAYGKPVGTAARVNVDQILMTLDTSEQYIVPAREALRKAGMKLSAPC
                     VINVEKGAELLH"
     gene            complement(2790..>3881)
                     /locus_tag="VN377_06400"
     CDS             complement(2790..>3881)
                     /locus_tag="VN377_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWR28048.1"
                     /translation="ALPGPEVALVLPGINPILPLEYLGYVLLGLVVAIIVHEFSHGIL
                     TLVDKLKVKSLGILYLIVPIGAFCEPDDEEVKKASILTRMRLYAAGPTANFVVVLICI
                     LLFSGVCMSAVQPMEEGGIIFTVDADSPASQIGLQSGVIVTSMNNSVIHNGEEFFTTW
                     NNTRANQTINVTYVKSGVEHTTEVLLGDKYIEYAKRPTIYTNNVSYKGKGYLGVQSLL
                     RDSVFKEYLAILKNPFFNFPQGLLTFYSLPLLGYFAGYNPIISPFADSYRITGPLGVL
                     PVPLFWIIVNAIYWIFWLNLAVSLFNVLPMIPLDGGFLFNDGVRAVILRFKKGVKEET
                     KEKIVKNVSLAISLLILGLILIPFFVKYI"
BASE COUNT         1076 a          834 c          840 g         1131 t
ORIGIN      
        1 gcgcttgacg acctgaagcg tattcatctg tatgcaaggt ccattcaagg gaaggcaaca
       61 acgcaagcag cggccgtcgt gatcctgagg atgaaaggaa tgggatatca ggaataataa
      121 aatcccgtgg ggcgatctcc tgcgaatcga taatagcatg ttcgattttt cccatttcac
      181 catccgcata catgctatcg tatccgacaa gaagaccggt gacgaccgct tttttcttcg
      241 atagttgggc attgacttta tcgatgtttg attctgtaac cgggattgct tcattgacaa
      301 taacgttctt tctcatagga taaatgatgt ccccgatgac tgcctgatga agcggaaggt
      361 tacgtttgat acgctcacta agtattttat taaacaacaa tgattcatag gcgtttacaa
      421 acatcatcag aagatttttt ggtagttctt tgaaagcacc aatgaagtct tggggatcct
      481 ggatcagttt attcaatatt gctttttcaa aatttaatgt ctcagggtag gaatggaatg
      541 cttttgtgta gtctcgggtt ttttcaagtt ctgttcgtaa tgcgaaggtc gtctcatttt
      601 ccccagacat cggattcccc acataagtca tggccgcttt ttcgaaatcc ccttgaataa
      661 tgtacttccc aacaagatgt gttattggtc ggataacccc gaatcgttga atcccataaa
      721 aattcggaaa accactccat ttctgaaaag gggaaatcag agagatgatc tgttctggca
      781 caatttcttc aggtatgttt ctgatcgtga tatcaaagtg attgcctaag agttctccga
      841 ttctcaccgg aacatctgat tggaatatgt tctcaattgt aacatccttg atgctaagcg
      901 atgacaactg ttctggagga acatgctcaa aagacatcaa ctgggtcgtc caggctcgtt
      961 tatccttggt tccagcaaaa ctgattcgtc tctgtgagag atgcaaacga tgcgctatct
     1021 cttgtacaag ggtatggttc tcccaatttc gactggtgac ctcagcgatc gtaaaatgtc
     1081 cttggtcttt cttgggtgga tataaaaaga gttcggttac gtggaaatca tcaatacgga
     1141 cccgaagttt tccatcaaca ccagggtggt ctgtacaata ggtatcgata ccgatctcgt
     1201 gttcaaaatc aggggcgttc atcaaagcga tgctctgtta tttttcaaaa ttcttcccaa
     1261 atagcttaat ttcatcctca aggtatttca ccgctttttt taagacgtcc tcaggtgagc
     1321 ctttttttac acggatgaat aaacgttttt tcactgcaag cggattgtcc gacatacaag
     1381 cagcatattc aacatcatca ttctgaagca agatatgagt gatcggatga agaatggttt
     1441 catcttcacc ggtgatttcc agttcaagtt cggtttttgt ctttcgaatg gttttcaaat
     1501 ccatattctc acaaaccctt ttcttttagt tataaatgtt gtatgtcaag attcccataa
     1561 tcttctgcga tgcgtcgatg ttctttgttc ccgcagttct cgcattcaag cccatggctt
     1621 tttttgataa gcggatgacg acactttgaa cacaatgcct tgattgctcc aaaattcctt
     1681 tcttttgtct caagctgaac gctcggtttt acttgaatga ctttcgcccg gatgtaatct
     1741 tccgatttgt actccgtgga tgcatctttg atgaatcctt gggcgatttc ctttacgtga
     1801 acagtagcat cagtgtcccc ggagatagac ctgttttttc ctgcgacatg aacgacatca
     1861 acgataatca tagttggttt tgcattggtc actcttgcga tgacgatatc tccttttttt
     1921 agcagcactg gtgtgcttga tgctggtttt acctcagcgg tacgatgttt ttcatcgact
     1981 acataggttc ctattcgcgc tgctcggata atcccatttt cttcaaatgt cccatctccc
     2041 ggtaataatt cctcagaagt ggagagttga tcaccgggca tgacaagttt atgtttcatg
     2101 ttttctcaca aatccaaata ggcaagctaa tctacgtttt aaaggttatg gtccgattat
     2161 tctcctaacg aaggattgat gatactggag taaaaagtct tataaatgac atgtttattt
     2221 cgatgttcct aagaatccag ttgaatgatg ggtgaaaaag tatgtcacgg aaaccagcga
     2281 gaatgatccg acaggtaaga ggtcaggcaa cgacaagacg agagtacatg ggcggtgttc
     2341 ccgcatcccg tctcacacag ttcgtcatcg ggaataaaac agcgaaattt cctgttcaat
     2401 tgtcattagt caccaaagag aaatgccagt tacggcacaa tgcgttagaa tcagctcgta
     2461 tctccgcgaa caagtctctt gagaaataca taggatctgc gaattatcga ttgcacatca
     2521 gagtctaccc tcatattgtt atccgcgaaa acaaacaagc gacaggagcc ggggcagacc
     2581 gtgtctccca aggtatgcga cgagcatatg gaaaaccagt cggaaccgct gctcgtgtga
     2641 acgtggatca aattttaatg acgcttgata catcagaaca gtacatagtt cccgcgcgag
     2701 aagccttgcg gaaagcaggg atgaagctct cagcaccatg cgttattaat gttgaaaaag
     2761 gcgctgagct tctccattag aaaccttttt taaatatatt taacaaaaaa gggaatcagg
     2821 attaatccca ggatgagtaa tgagattgct aacgaaacgt ttttgacgat tttttctttc
     2881 gtttcttcct ttactccctt tttaaaccgc aagatgacgg cacggacacc gtcattaaag
     2941 aggaagcctc catcaagcgg aatcattggc agcacattga agagggaaac cgcaaggttc
     3001 aaccagaaga tccaataaat cgcgtttacg ataatccaaa acaaaggaac ggggagaact
     3061 cctaaaggac cggtgatacg atacgaatca gcaaaaggtg agatgattgg gttgtatcct
     3121 gcaaagtacc caagcaaggg taggctgtag aacgtcagta gtccttgtgg aaaattaaaa
     3181 aacgggtttt tcaggatagc gagatactct ttgaagacag agtctcgcaa aagggattga
     3241 acccccaggt atccttttcc tttataggat acattattcg tatagatcgt cggtcgtttt
     3301 gcgtactcga tatatttatc gccaagaagc acttctgttg tgtgttcgac accgcttttt
     3361 acgtaggtga cattgattgt ttgattcgcc ctcgtattat tccacgtcgt aaaaaactct
     3421 tcaccgttat ggatcacaga attgttcata ctggtaacga taacccctga ttgcaaaccg
     3481 atttgtgatg ctggagagtc tgcatcaacc gtgaaaataa taccgccttc ttccatgggc
     3541 tgcactgctg acatgcatac gccggagaac agtaaaatac agatgagtac gacgacgaag
     3601 ttcgctgtcg gacctgccgc gtatagcctc atcctggtaa gaatgcttgc tttttttact
     3661 tcctcatcat ccggctcgca gaacgctccg atggggacaa tcagatacaa aatgccaagt
     3721 gattttacct tcaatttatc aacgagcgtc agaatcccat gggagaattc atgaacgatg
     3781 attgcgacga cgaggccaag cagaacatac ccgagatatt caagtggcag gataggatta
     3841 attccaggga gcaccaaggc aacctcaggt ccaggtaatg c
//
LOCUS       DATMXJ010000244         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_5023, whole genome shotgun sequence.
ACCESSION   DATMXJ010000244 DATMXJ010000000
VERSION     DATMXJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1003)
                     /gene="mptA"
                     /locus_tag="VN377_06405"
     CDS             complement(122..1003)
                     /gene="mptA"
                     /locus_tag="VN377_06405"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HWR28049.1"
                     /translation="MKSTDLPDVQSRLASSHFKLTRVGVTGVKKLVHIQRPNKKSVLP
                     LTVTLDLFVDLPASQKGSHMSRNLELISEIIDQTLEAPVSDLESFCATTAELLLKKHE
                     YATFSEVKAEADYFLDVVYPSGQTGMEPYNLIAEARANRGGELRKLIGVKVIGMTACP
                     CAMEVVRKLEARDKTCSAPTHNQRNIGTLMIEVPRGTEVVDADDLIDIVEKSFSSPTY
                     SILKRKDEAELVLRAHTKPKFVEDVVRDILSSVLKKYRDLPDEVLVIARSESEESIHK
                     HNAFAERVTSLGDLRKT"
     gene            1180..1815
                     /locus_tag="VN377_06410"
     CDS             1180..1815
                     /locus_tag="VN377_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWR28050.1"
                     /translation="MRRSQNGAMGIGVMIIFIAMVLVAGIVAYAIFSTGSLLEIQSGS
                     TGSQTIKEVSSGLKVSTIQGHNTSGLIDKMVILIIPRAGSPEIDLNGVLIELSDSDQK
                     NVLEYSSLYWVDGTAGLDNLFDVDAFPSVSSEYGVIVLIDDDDSCNQTTPVINRGDSM
                     MLALNTTVIFGGIGENVNIAGNVIPEEGAWSIIQFRTPSSFVNPILILQQD"
     gene            1931..2002
                     /locus_tag="VN377_06415"
     tRNA            1931..2002
                     /locus_tag="VN377_06415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1963..1965,aa:Thr,seq:ggt)
     gene            2125..2394
                     /locus_tag="VN377_06420"
     CDS             2125..2394
                     /locus_tag="VN377_06420"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWR28051.1"
                     /translation="MSEVDVFKALADPTRLKILECIKDGEKCICEVIPYTGKSQPNVS
                     QHLKVLKIAGLVDERREGTRILLKASDKKIFDVIDSVKKMKSITA"
BASE COUNT          670 a          457 c          555 g          764 t
ORIGIN      
        1 gtctttcccg acgccatctc ccgggagtac ggcaatataa tatttcgaca tggaagacga
       61 taagcttctg ttgttttaaa gcttttttgc cctaaaaaaa tttttctcac taccgctgta
      121 gttatgtttt ccgtaaatca ccaaggcttg ttactcgctc ggcgaacgcg ttgtgtttat
      181 gaatcgattc ttcgctttca ctacgggcta tcactaaaac ctcatctggt aaatccctat
      241 atttttttaa cacagaacta agaatatcac gaaccacgtc ttcaacgaac tttggtttgg
      301 tatgtgcccg aaggacaagc tcagcttcat ctttccgttt taatatacta taggtcgggc
      361 tgctgaatga tttctcaacg atatcaatga gatcatctgc atcaacaacc tcggtacccc
      421 gtgggacttc aatcattaat gtaccgatgt ttcgttggtt atgagtggga gcagagcagg
      481 ttttatcgcg ggcttcaagt tttcgaacga cttccatagc gcagggacaa gcggtcatgc
      541 caatgacttt gacgccgatg agtttcctta gttcaccgcc acggtttgcc cgtgcctctg
      601 cgatgagatt gtacggctcc atgcccgttt gaccagaggg gtacacgaca tctaaaaaat
      661 aatcagcctc tgctttcacc tcagaaaacg ttgcatattc gtgtttttta aggaggagtt
      721 cagcggtcgt ggcgcaaaat gactctaaat cagatactgg tgcttcgagt gtttgatcga
      781 taatttcaga gattaactca aggtttcggg acatatggct gcctttctga gatgcaggga
      841 ggtccacaaa caggtctaat gtgacggtga gtggaagaac agatttttta tttggtcgtt
      901 ggatgtgaac cagttttttt acgccggtta ccccgactct tgtgagtttg aaatgggagg
      961 atgctagtct tgattgtaca tctggtaaat cagtggattt cataggagtc aacgacagcg
     1021 gaatactctt atatctcaaa tacattcgca ttttactcag atttgttcaa aaaaggatct
     1081 aggttatctc tcctcgtttc tccagttttt cataatactc aaatagtacc ttttctttac
     1141 atgttattga agggaataga ggggtatctg catacgaacg tgagaagaag tcagaatggg
     1201 gctatgggga ttggtgtcat gattattttt attgcgatgg tccttgttgc agggattgtt
     1261 gcctatgcga ttttttccac aggttcttta ctggagattc aatcagggag tacagggtcg
     1321 cagaccataa aagaagtctc gtcaggtttg aaagtatcaa cgattcaggg tcataacacc
     1381 tcagggttga ttgataaaat ggttattctc attatccccc gagctggttc tccagaaatc
     1441 gatctgaacg gggtgctgat tgaactgtct gattctgatc aaaaaaatgt actggagtat
     1501 tcatctctgt attgggttga tggaaccgcg gggttagata atcttttcga tgtcgatgcg
     1561 tttccttcgg tctcctcaga gtatggggtt attgtcttga tcgatgacga tgattcctgt
     1621 aatcaaacca cgccggtgat caatcgagga gatagcatga tgttagcgct taatacgact
     1681 gtaatctttg gtggtattgg tgaaaatgtg aatatcgcgg gtaatgtcat ccctgaggaa
     1741 ggagcatgga gcattattca attccgaaca ccttcttcct ttgtgaatcc tatcctgatt
     1801 ttacaacagg attaagttgt tgcaggtggg tgtgttttta ttatgtttct tttttgtttt
     1861 tcttcgtagt tcagattttg aaggaaaaaa ggtaaataac ccccctgtta ttcagggcag
     1921 tatctgaaat gccgctgtgg cttagaggta tagcgattcc ttggtaagga ataggtcgag
     1981 ggttcaattc cctccagcgg cttcaagtta acaattggca ttgttttaaa ctggagaaaa
     2041 acatcagcaa tctggatttt tatacgattt ttaaagagta aatatagtaa tatttaaata
     2101 ttcaaattta cttatatacc tactatgagc gaggttgatg ttttcaaagc attagctgat
     2161 ccgacacgac tgaaaattct cgaatgtatc aaagatggcg aaaaatgcat ctgcgaggta
     2221 ataccctata cagggaaatc gcaacccaat gtttctcaac atttgaaagt gctgaaaatc
     2281 gcaggtctcg tcgatgaacg aagagaagga acaagaattc tgcttaaggc atcggataag
     2341 aagattttcg atgttattga ttcagtaaaa aaaatgaagt cgataaccgc atgaaaaaaa
     2401 ttttctttac agagggaaaa tctgcgtgag cggtaaggat agtaaa
//
LOCUS       DATMXJ010000245         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36413, whole genome shotgun sequence.
ACCESSION   DATMXJ010000245 DATMXJ010000000
VERSION     DATMXJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36413"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VN377_06425"
     CDS             <1..656
                     /locus_tag="VN377_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWR28052.1"
                     /translation="QNYLPYEGSAMIQDGTNSPPLKPATPVGPSNGGINIEYTYSTST
                     TDPDQDQVWYQWQFGSYTTNWFGPYSSGAQTQTQYAWTTPGTYEIKVKAKDQNQYESE
                     WSSPLFVTITDLKPLLTIGAVNGGLFSITSAINNIGEGIATNVVWTITVDGNFILSGQ
                     TLSGTMSTLDVNSTATVENSPILGFGNVIITVRAQADGVSEITKTVDGFIFFIYVII"
     gene            766..1959
                     /locus_tag="VN377_06430"
     CDS             766..1959
                     /locus_tag="VN377_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWR28053.1"
                     /translation="MEQKLYRYRWAVVGIFWFVIFAYGANWFALSPMLKAFETQFLVP
                     DWEAHLLISLIGMFVIFFAWPAGSLIDRKGPKLSISIGALFMALGFGLRPWLLTSFET
                     LIISSVIAGIGLAWILVALAPQMFRWFPAKHAGLPVGIGASGLFIGFGTGSLCMPLLT
                     PDKTSSQIFNGFLIFGVLAVIAFILWLVIAKDHPATPPEERVKIEKMRFTEGMKHVFS
                     SKNAYIYPFIGFFIVGITLVISALLHQLYPGQDGGYIAGLLLYGCAIGAFFAPFLAKK
                     IGLKKVTLSVVFGAVIFWLIIFVLNYYYDATWLLVLPFAFLFGICFQASWPLALYSQE
                     TENGVTESNVGIAASLYISISNIGAAVLPVIFPLLFIDFFQNFIAILIGLIICLLLWV
                     VVKRK"
     gene            complement(1964..>2599)
                     /locus_tag="VN377_06435"
     CDS             complement(1964..>2599)
                     /locus_tag="VN377_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HWR28054.1"
                     /translation="EEMRKNIERRWGIVAHEHYGLTELIGPGVVSECEYKKLHINADH
                     FYPELIDPLTGETLEYGEEGELVYTTLTKEAFPAIRFRTKDLASITEEICECGRTLPI
                     QSRIKGRSDDMMKIKGVIVFPSQIEAALMQVSGVSDNYQIVKTKQGDMYSLSVEVEPS
                     EARYKEGHLDDFEKKAENEIYRILNLHVPVHAISPKAIPRSTGKAKRVIER"
BASE COUNT          639 a          611 c          568 g          781 t
ORIGIN      
        1 ggcagaacta tcttccctat gaaggatcgg caatgataca agatggaacg aatagcccac
       61 cgctgaaacc agccacgccc gtagggcctt cgaacggagg aatcaacatc gaatacacgt
      121 acagtacgag cacgacggac cctgaccaag accaggtgtg gtaccagtgg caattcggct
      181 catataccac caattggttc ggcccctatt cctcaggagc acagacacag acacaatacg
      241 catggacgac cccggggact tatgaaataa aagtaaaagc aaaagatcaa aatcagtatg
      301 aaagtgaatg gtcttctcct ctgtttgtca ccattactga tctgaaaccg cttctgacga
      361 tcggggcggt gaacggcgga ttgttttcca ttacctctgc catcaacaat atcggtgaag
      421 ggatcgccac aaacgtcgtc tggacgataa ctgtcgatgg gaatttcatc ctttcaggac
      481 aaaccctctc aggaacgatg tcaactcttg atgtaaactc taccgcaacc gtcgaaaact
      541 caccaatact tggttttgga aacgtcatca tcaccgtaag agcacaagca gatggtgtgt
      601 ctgaaatcac aaaaacagtg gatggattca tcttcttcat ctacgtcatc atataaaaga
      661 taagattttt tctcttttta cttttttgaa tggtaatatt ttttgtttga ttttgtcggt
      721 aactataaaa acatcattaa tatcatcgtg ccggagaggt attctatgga acagaaactg
      781 tatcgatatc gttgggcagt tgtcggcata ttctggtttg tgatctttgc ctatggggca
      841 aactggtttg cactatcacc aatgttaaaa gcctttgaaa cacagttcct tgtcccagac
      901 tgggaggcac atctcttgat ttccctgatt ggaatgtttg tgattttctt cgcctggcca
      961 gcagggtccc ttattgacag gaaaggaccg aagctttcca tctcaattgg agcgctgttt
     1021 atggctcttg gttttggtct tcggccttgg cttcttacta gcttcgaaac tctcattata
     1081 agcagtgtca tcgctgggat tggacttgca tggatcctgg tcgcactagc acctcagatg
     1141 ttccgatggt ttcctgcgaa acatgcagga ctcccagttg gtatcggtgc ctcgggattg
     1201 ttcattggat tcggaacagg ttcgctgtgt atgcctcttt taacgcctga taaaacatcg
     1261 tctcagattt tcaatggttt tttgatattt ggcgtccttg cagtaatcgc tttcatcctt
     1321 tggctcgtca tcgcgaagga tcatcctgcc actcccccgg aagaacgagt gaagattgag
     1381 aaaatgaggt ttacggaagg aatgaaacat gtttttagtt caaaaaacgc atatatctat
     1441 ccttttatcg gttttttcat tgtggggata acacttgtta tttcagcgct tctccatcag
     1501 ctctaccctg gacaggatgg tggatatatc gctggtctcc tcttatatgg atgcgcgatc
     1561 ggtgcatttt tcgccccgtt cctcgcaaaa aaaattggat tgaaaaaggt gacgctctct
     1621 gtagtgtttg gagcagtcat cttctggctc atcatattcg tcttaaacta ttattatgat
     1681 gcaacctggc ttttggttct tccattcgcg tttctctttg gaatctgctt ccaagcgtct
     1741 tggccgttgg ccctttatag tcaagaaaca gaaaacggtg tcacagaatc caatgtgggt
     1801 attgctgcaa gtctgtatat ttccattagc aacattggtg cagcggttct cccggttatc
     1861 ttcccgcttc ttttcataga tttcttccag aatttcattg ctatcctcat tggactgatc
     1921 atctgccttc tcctctgggt agttgtaaaa agaaaataat tcgttatcgt tcaataacac
     1981 gttttgcttt cccagtgctt cgaggaatgg cctttggtga gatcgcatgg accgggacat
     2041 gtaaattgag gattcggtaa atttcgttct cagctttttt ctcaaagtca tcgagatggc
     2101 cttctttata gcgagcttcc gatggctcaa cttcgacgga aagcgagtac atatcccctt
     2161 gcttggtctt gacaatttga tagttatcgc tcactccaga gacctgcatt aaggcagcct
     2221 cgatctggga ggggaacaca atgacccctt tgatcttcat catatcatcg ctacgcccct
     2281 tgatccttga ctggatcggt aatgttcgtc cacactcgca gatctcttcc gttatggacg
     2341 cgaggtcctt tgtccggaaa cggatcgcag ggaaagcctc ctttgtcaag gtcgtgtaca
     2401 caagttcacc ttcttcacca tattcaagtg tctcccctgt gagtggatcg atcaattcag
     2461 gatagaaatg atcggcgttg atatgcaatt tcttatattc acattcggag acgaccccgg
     2521 ggccgatcag ttcagtcaac ccataatgct catgggcaac aatcccccag cgtctctcga
     2581 tgttcttccg catctcttc
//
LOCUS       DATMXJ010000246         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_29969, whole genome shotgun sequence.
ACCESSION   DATMXJ010000246 DATMXJ010000000
VERSION     DATMXJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29969"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VN377_06440"
     CDS             <1..219
                     /locus_tag="VN377_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28055.1"
                     /translation="AIYLYKWITVVQQDFIRKKTTVHCMIVIGLLSTEEEAQEIQFIA
                     KICTVENRIRYLSHVGRIKKLVNEESKL"
     gene            260..1132
                     /locus_tag="VN377_06445"
     CDS             260..1132
                     /locus_tag="VN377_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWR28056.1"
                     /translation="MTVLIISSSEDPASTHIKNSLLEQTTWKEQGFFYNTPVYRHTSL
                     KNLVMVTIPDKKIRHENIDTEVQEQLHIDSTIAIFLSRHRSKTGEPTLTVHPIGNFSD
                     ALFGGQPKTLVPAAPNMMTHLLRLINKNLQMTDLDYKVCYEVTHHGPFLRIPTFFAEV
                     GSTEVQWQQKEPASIIATSLLELLRTHQHEQEFPKDIPVILGVGGGHYAPRFTDIAFQ
                     KKIAFGHMIPSYKIDEGTIDDEILEKTMQSTPNLTGVYLHKKDLKKSQVTHFRNWFEK
                     KGVSLISSKDLLDL"
     gene            complement(1127..1432)
                     /locus_tag="VN377_06450"
     CDS             complement(1127..1432)
                     /locus_tag="VN377_06450"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWR28057.1"
                     /translation="MRQIPAGRVSTYGAVAKALGNKKYARVVGTYLNKNPDADTMPCF
                     KIVKSDGSLGGFGLGINDKIRRLQNDGIEVKNGKIIDFEHVFFDDFKTEYPLKKNKS"
     gene            1512..2282
                     /locus_tag="VN377_06455"
     CDS             1512..2282
                     /locus_tag="VN377_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWR28058.1"
                     /translation="MNTIKFLGTAGARFVVMKQLRASGGIWITLDETHVIIDPGPGAL
                     LRCLSSRPKLDPQDLEGIILTHRHLDHSNDINIMIEAMTNGGFKKKGTVFAPSDALQG
                     DPVILKYAREQVDHIEILKEKESYKVGNISFETPKQLKHGVETYGLNIRGKNSTISLI
                     SDTDYFTELPSFFTGDILIINVVMLEDRSTIEHLCLNEAELIIKTNKPRLAILTHFGM
                     GMVKAKPWEIAEHLTQKLGIQVLAAQDGMQIDIDQYTI"
     gene            2315..3304
                     /locus_tag="VN377_06460"
     CDS             2315..3304
                     /locus_tag="VN377_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR28059.1"
                     /translation="MTVRSLLPKYHNILEENGTAQYLDCKHIKVRFDAMQPANELWEL
                     HDKALRSFKVLNTIQSPSLLDLKIELADRLFQACCFCEHRCKVDRRKTLGVCGVQEAH
                     IATEFLHFGEESVLVPSHTIFFSGCTFQCVFCQNWDISQLPCGTFIDPEKLGKKIEER
                     RQQGARNVNWVGGDPTSNLLYILQVLQQMDTNIAQMWNSNMYCSEETMKLLHGVIDLY
                     LTDFKYGNDSCATRLSKVNGYTAVVKRNHLLAYNHGEMLIRHLVLPNHIDCCSKPVLR
                     FIAKHLPDALVNIMGQYKPEYRAYEQKDISRSISSEEVLRVKEYAKKLGIHQI"
     gene            complement(3404..4168)
                     /locus_tag="VN377_06465"
     CDS             complement(3404..4168)
                     /locus_tag="VN377_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HWR28060.1"
                     /translation="MTEQWRLLQTGFKNAYSNMAIDKAILVVNSEGNVPPTVRFYGWS
                     PPAISIGYFQSLTEEIDIQACERFGIDYVRRITGGGAVFHEKELTYSIVVAESHPAIP
                     KNILKSYGRICGAVMKGLLQLGIESEYAPINDIVTGGRKISGNAQTRKFETVLQHGTV
                     LMDVDVDTMFSLLKVPNEKIKDKLIADVKQRVTSMKHILGKEIGFQEVARAMKIGFEE
                     EFNVELIEGTLSKEERGLAIQFEEESFSTKEWNQRR"
     gene            complement(4165..4449)
                     /locus_tag="VN377_06470"
     CDS             complement(4165..4449)
                     /locus_tag="VN377_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013142955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWR28061.1"
                     /translation="MQSYSIYKVPQGKLLKISLEYDEKMNVITDICITGDFFAYPEEA
                     IELLEKKLKNTMLDHVHLLEKISSLIAEYHIQFIGVNAEGLTQGILMCRP"
     gene            complement(4503..>5335)
                     /gene="lipA"
                     /locus_tag="VN377_06475"
     CDS             complement(4503..>5335)
                     /gene="lipA"
                     /locus_tag="VN377_06475"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWR28062.1"
                     /translation="DWLKVRIGSGENYSKVKTLLRSAKLHTICEEAKCPNIAECFGSG
                     TAVFLILGNICTRNCRYCNVTHGTPGPLNNDEPSDIAESVKTLDLKYVVVTSVTRDDL
                     TDGGASVFYKTVRAIRRGNQECRIEVLIPDFKGDEAAIQKVIDAVPDVINHNIEVVED
                     LFPLIRPQGNYLRSIDLLKTLKMKGKGIRVKSGLMVGLGESTEQILATLYDLRASGVD
                     FLTLGQYLQPTHNHAIVKKYYPPEEFLQLRTAALQLGFAHVESGPLVRSSYHAQNALQ
                     "
BASE COUNT         1561 a         1243 c         1069 g         1462 t
ORIGIN      
        1 gcaatatact tatataaatg gataacggtt gtacaacaag actttataag aaagaagaca
       61 accgttcatt gcatgatagt catcggactt ttatcgacgg aagaagaagc acaggaaatc
      121 caattcatcg caaaaatctg caccgtggaa aaccgaattc gttatctttc ccatgttggc
      181 aggattaaaa aactagtcaa cgaggaaagt aaactgtgac gcaaattaca aaaacaccct
      241 gcttgttctc ctccagtata tgaccgttct gattatttca tcatcagaag acccagcgag
      301 cacgcatata aaaaacagtc ttttagaaca aacgacctgg aaggagcaag gtttttttta
      361 taacacccct gtgtacagac atacatccct gaagaatctg gtgatggtga cgatccctga
      421 taaaaaaatt cgacatgaaa acatcgacac agaagtacaa gaacagcttc acatcgactc
      481 aacaatagcg atctttctgt ccagacatcg cagtaaaaca ggggagccaa cactgacagt
      541 ccatccgatt ggtaacttca gtgacgcctt gttcggcggg cagccaaaga ccttggtacc
      601 tgctgcacct aacatgatga cccatctact cagactcatc aacaaaaacc tccaaatgac
      661 agatcttgac tataaggtct gttatgaggt cacccaccat gggccgttcc tgaggattcc
      721 cacttttttt gcagaggtgg gcagcacaga ggtccaatgg caacaaaaag aacctgccag
      781 catcatagca acatcactgc tcgagctgct acgcacacat cagcatgagc aagagtttcc
      841 aaaagacatc ccagtaatcc ttggagtcgg aggagggcac tacgcgccac gattcaccga
      901 tatcgcgttt cagaaaaaaa tcgccttcgg tcacatgata ccttcctata agatagatga
      961 aggaacaata gatgatgaaa tccttgaaaa aacaatgcag tcaacaccaa atctgacagg
     1021 agtatatctc cataaaaaag acctgaaaaa atcacaagtg acccatttta ggaattggtt
     1081 tgagaagaaa ggcgtctctc ttatctcaag taaagacctg ctagatttat gatttgtttt
     1141 tctttaatgg atactctgtt ttgaaatcat cgaaaaatac atgttcaaaa tcaatgattt
     1201 tcccattttt tacttctatg ccatcgtttt gtaatcttcg tatcttgtca ttgatcccaa
     1261 ggccaaaccc acccagactt ccatcagatt tgacgatttt aaaacacggc atggtgtctg
     1321 cgtctgggtt tttgttcaga taggtaccaa cgacccttgc gtattttttg tttccaagag
     1381 ccttcgcgac cgcgccatag gttgatacac gtcccgcagg tatttgcctc acaagataat
     1441 aagtataatc ggaaagattc atatgtcctt ataggctatg acctaaaaaa aattttctat
     1501 agatggatac gatgaataca ataaaattct tgggaaccgc tggtgcacgt ttcgttgtca
     1561 tgaaacagct tcgagcatct ggtggtatct ggatcacctt ggatgagacc catgtaatca
     1621 ttgatcctgg tcctggcgcg ctgcttcgct gtctctccag caggccgaag ctggacccac
     1681 aggacttgga agggatcatc ttgacgcatc gtcatcttga ccattccaat gacatcaata
     1741 tcatgattga agcgatgaca aacggcggat tcaaaaagaa aggtaccgta ttcgccccaa
     1801 gtgacgcact ccaaggggac cccgtgatcc tcaaatatgc aagagaacaa gtcgatcata
     1861 tcgaaatcct caaagaaaaa gaaagctaca aggtggggaa catctccttt gaaacaccaa
     1921 agcagctgaa acatggagtc gaaacatacg gtttgaacat caggggaaaa aacagcacga
     1981 tctcccttat ttccgacact gattatttca cagagcttcc ctcttttttc accggcgaca
     2041 tcctgattat caatgtcgtg atgttggaag acagaagcac tattgaacat ctctgtttaa
     2101 acgaggcgga gctaatcata aaaacaaaca aaccacgtct cgcgattctt acacattttg
     2161 ggatgggtat ggtcaaagca aaaccctggg agatagcaga acaccttaca cagaagctgg
     2221 gtatccaggt tctggcagcc caagatggta tgcagattga tatcgatcaa tacacaatat
     2281 agttttaatt ctccccaacg atatcaaaaa tagaatgacg gtacgatcgc tccttccaaa
     2341 ataccataat atccttgaag aaaatgggac cgctcagtat cttgattgta agcatatcaa
     2401 agtacgtttt gatgcaatgc aaccagccaa cgaactctgg gaactgcatg ataaagcact
     2461 ccgttcattt aaggtcctga acacgattca gtcaccttcg cttcttgatc taaaaatcga
     2521 gcttgctgac cgtctctttc aagcatgttg tttctgtgaa cacagatgta aagtcgacag
     2581 gaggaaaacg cttggggtgt gcggtgtcca agaagcacac attgctactg agttcttaca
     2641 tttcggtgaa gaatccgttc ttgtcccgtc gcataccata tttttctcag gatgcacgtt
     2701 tcaatgcgtg ttttgtcaga actgggatat cagccagtta ccttgtggaa cattcataga
     2761 tccagaaaaa ctaggtaaaa aaatagaaga acggaggcaa cagggtgctc ggaatgtgaa
     2821 ctgggttggt ggagatccga catcaaatct cttgtacatc cttcaagttc tccagcagat
     2881 ggatacgaac atcgcccaga tgtggaactc gaacatgtac tgttcagagg agacaatgaa
     2941 attactccat ggagtcatcg atctctatct gaccgatttt aaatacggga atgattcctg
     3001 tgcgacacga ctctccaagg tcaatggata tactgctgtt gtgaagagga atcatctgct
     3061 tgcctataat catggtgaga tgctcatacg gcatctggta ctccccaatc acatcgattg
     3121 ttgttcaaaa ccagtcttaa ggtttattgc gaaacaccta ccagatgctt tggtcaatat
     3181 catgggtcag tacaaaccag agtacagggc gtacgaacag aaagacatat cacgatcgat
     3241 ttcatctgaa gaggtcttgc gggtcaaaga atacgctaag aaacttggaa tccaccagat
     3301 ataatctcgt atgacatcgt ctttctatct accaggttct tctacgtgaa agtgacctga
     3361 tacctacaag accattcttg ataatgacga agtcaaaaac acctcaacgt ctctgattcc
     3421 actcctttgt agagaaagat tcttcttcaa attgtatcgc cagacctcgc tcctctttgc
     3481 ttaatgttcc ctcaatcaac tcgacattaa attcttcctc aaaacctatc ttcattgcac
     3541 gtgcgacttc ctgaaaacct atctcttttc ctaaaatgtg cttcatggaa gtgactcgtt
     3601 gtttcacatc ggcaatgagt ttatccttaa tcttctcgtt tggtactttc agcaatgaaa
     3661 acattgtatc aacatcgaca tccatgagga cggtcccatg ttgcaacaca gtttcaaact
     3721 ttcttgtctg agcgttccca gaaatcttcc gaccaccggt aacgatatcg ttaatcggag
     3781 cgtactcact ctcaatcccc aactgaagca accctttcat gaccgctccg cagatcctcc
     3841 catagctttt caggatgttt tttggaattg ctggatgaga ctcagcaacg acgatactgt
     3901 acgtcaattc tttttcatga aacacagcgc ctccgccagt gatccttcgt acgtaatcga
     3961 tgccaaaccg ttcacaggct tggatatcga tttcctcggt caggctttgg aaatacccaa
     4021 tagaaatagc aggaggagac catccgtaaa accgtacggt aggaggaacg ttcccttcac
     4081 tatttacgac caggatggct ttgtctatgg ccatatttga atacgcattt ttaaaacctg
     4141 tctgaagcaa cctccattgt tctgtcatgg tctgcacatc aatatccctt gggtcaggcc
     4201 ttctgcattg acaccaataa attgaatatg atactcggca ataagggaag aaatcttttc
     4261 caataagtgc acatggtcaa gcatggtatt ttttagtttt ttttccagaa gctctattgc
     4321 ttcctcaggg taagcaaaaa aatcaccagt gatgcaaatg tcggtgatga cattcatttt
     4381 ttcatcgtac tccaatgaga ttttcagtaa tttcccctgg ggcaccttgt agatagagta
     4441 cgactgcata tgtcttatgt gtatttgatt ctcttttaaa aggttccccc atcaatacac
     4501 aatcactgca aggcgttctg tgcatgatac gagcttcgca caagcggccc tgattcgaca
     4561 tgcgcaaaac caagctgtaa cgcagcggtc cttaactgta aaaattcctc gggcggataa
     4621 tattttttca cgatggcatg attgtgtgtg ggttgaagat actgtcccag ggtcaagaaa
     4681 tcgacccctg atgctcgcaa atcatacagt gtcgcaagga tctgctctgt ggattcccca
     4741 agtcccacca tgagaccgga tttcacccga atgccctttc ctttcatttt tagtgttttt
     4801 aacagatcta tactccgcag atagttccct tgaggtcgta tcagcggaaa caaatcttcg
     4861 actacttcga tattatggtt aatgacatca gggactgcat caatcacttt ttgtatcgct
     4921 gcttcatctc ctttgaaatc ggggatgagc acttcaatgc gacattcctg atttccgcgt
     4981 cttattgctc gcacggtttt ataaaaaacg cttgcaccac catcggtgag atcatcccgt
     5041 gtcactgacg tgaccacgac atatttcaga tctagggttt ttacgctttc agcaatatca
     5101 ctgggttcat cgttgtttaa gggacctggt gttccatgtg tcacattgca atagcggcag
     5161 tttcgagtac agatgttgcc gagtatgaga aatactgcgg tgccactgcc gaaacattca
     5221 gcgatgttag ggcattttgc ctcttcgcag atcgtatgga gtttcgctga tcgcaacagc
     5281 gtttttactt tagaataatt ttctccgctc ccgatacgta ctttcagcca atccg
//
LOCUS       DATMXJ010000247        10220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_51655, whole genome shotgun sequence.
ACCESSION   DATMXJ010000247 DATMXJ010000000
VERSION     DATMXJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51655"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VN377_06480"
     CDS             <1..688
                     /locus_tag="VN377_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441722.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HWR28063.1"
                     /translation="HNAIPREAYAKLVLDKKNKGSFVSEIKAEEKEIYDEIKPIDPNL
                     KVDVSSCESSKTAMEKSSQVKLLNLLQGLPHGVHQMNYDIKTLVNTSTNLATVSVKEN
                     TIVIGMSSRSPMKSALQDMRDRIKAIASLSGAKVSEGTPYPGWKPDLQSKILALSKKT
                     FKDMFKTDAKIEAIHAGLECGIIGEKFPGMDMISIGPTLKNPHSPEEQLHISTVDKFY
                     RYLLKILESV"
     gene            698..1402
                     /locus_tag="VN377_06485"
     CDS             698..1402
                     /locus_tag="VN377_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWR28064.1"
                     /translation="MPPRCSFSIIIPVLHESKIINTLLDSLDRLKRDEPFEIIVVDGS
                     PTQDTLQVISKKNVMKYVSPQGRGRQMNEGAAHSTGDILIFLHADTFLPSNALTLIQT
                     TLADQRYVGGAFALGIQSQNPLLKMIASFSTLRSRLTRAPYGDQVIFLRKSYFDAIGG
                     YRDIPLMEDVELMRRVKKKDGEIIILSTSVITSDRRWNQEGLLYTTVRDLIIIFLYWC
                     GIPAEKLVKFYPWQKE"
     gene            1501..2460
                     /gene="arsS"
                     /locus_tag="VN377_06490"
     CDS             1501..2460
                     /gene="arsS"
                     /locus_tag="VN377_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HWR28065.1"
                     /translation="MSEKKNEFEKRIHDVTHDGLQCLDLRTIQINLGLLCNQTCEHCH
                     VNGSPKRTEQMSWPTMQTILSMTETLSPLLIDITGGAPELHPHLEKFLRSLAKQRHRI
                     QLRTNLTILLDPAYKHFISLYKELGIELVASFPCYLEKEVRLQRGKGVFEKSIEALQL
                     LNKIGYGTTPDLPLILVFNPLEPVLPPEQKTLENEYRIYLAEHYKIQFTRLITLTNMP
                     LGRFLNVLKEKKPQYLRLLKESFNPETLPSLMCRHQINIAWDGALYDCDFNLAAGIPV
                     TSAVPRHIQQFKKSAISTRKIATADHCFGCTAGQGSSCGGALV"
     gene            2482..3207
                     /locus_tag="VN377_06495"
     CDS             2482..3207
                     /locus_tag="VN377_06495"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HWR28066.1"
                     /translation="MKESNQKNDWSKGWWKPVVLIIVIVALFIVMNLYGFGQNITEVQ
                     HWIQSLGIWGPVAFFIIYLGSVIATIPGTIFGVIAGGLFGSVIGVILISISSTVGASI
                     CFLIARYFARDTVARWFSKNKTMKRLDELTQDQGMMIVGLTRLIPLFPFTLLNYGFGL
                     TKVSFKTYVFWSWLCMLPGTIIVVVGTDALTQGLTQGRVPWELLCVLIITIIALGILV
                     WYSRRTLKEKRKNKEEQEKNKQE"
     gene            3237..4373
                     /locus_tag="VN377_06500"
     CDS             3237..4373
                     /locus_tag="VN377_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWR28067.1"
                     /translation="MNGQTEKNKESVPTKVFLQRKVSEYIERCIECNRCMDVCPVTKD
                     IFSIHELNVASQEGQSVPLKIKEFAFHCMQCGQCVPVCPKNIRRDHMVRYIKYKIRGE
                     KPWSYKRYLLIKGPNQTGIRRTIQQLFIRSKKLTNKDLAYFMETIPEKKAEVLFYPGC
                     YIYSTKTVQQTLRLLKHIGCSYTVLGGVTTCCGTPHLLQGEFDQADDCLSLLYQKIKA
                     CDPQIILTACAECFEAVEQIKKRYTMDVEVLSVTQYLLRYQDKFPSKKIRGKITVHDS
                     CRFHAQSPQGVAVHDVVSRFGELVKPPIDQPSNCCYQWNHGSDTRNVLRRANYLAAVK
                     NNAPTLACNCLTCYEEFKKTHTDTEIIDALQLFEEALQPSETRE"
     gene            4376..5098
                     /locus_tag="VN377_06505"
     CDS             4376..5098
                     /locus_tag="VN377_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HWR28068.1"
                     /translation="MMTKDQDTCILLFVKYPEKGKVKLRLSRGLNEEIVQELYRCFVQ
                     DTLTTIKKIDAPFFICFHPPEAQSKFQSWLGSTLRFLPQQGEDLGERMKNSFTDVLTK
                     GFQKVILMGSDSPDLPEDYIKQAFTTLQTKDAVLGPTVDGGYYLIGFKTTTFTPNVFE
                     EIHWSSPLVFQETMMKLKQAQRSVGLLPVWSDIDTPSDLHNLVRRTRNTSFKSSNTMT
                     YIHQHQIQVESNHEEKSGTKPN"
     gene            5067..5495
                     /locus_tag="VN377_06510"
     CDS             5067..5495
                     /locus_tag="VN377_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28069.1"
                     /translation="MKKNQELNQTEVFLADKKTGLWYSLYLSIASVSLFASMVALNTA
                     YQGFIWSYSTRYVINSFILAFALFISGAFLIVACMKLLKKRPSARLFGFVGIGFLIAY
                     SLFILIIDRYIAYTLVYVLMLLILSVVIMAGAVFFYKKSF"
     gene            complement(5518..6396)
                     /locus_tag="VN377_06515"
     CDS             complement(5518..6396)
                     /locus_tag="VN377_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR28070.1"
                     /translation="MKKTRFLVIVILLFLCTFSFFLSPTTGEDITTPIVYVGGSGPGN
                     FTQIQNALDSATTRGTVYVYPGTYHENLRIIQPVHLIGDDTHNTIIDGDNKDYVITLA
                     AGNSTVSGFTITHSELKFPFAGVYVVSGHNTISHNILTDNFYGMQLGYSTSDNLIVNN
                     TIHHNGRCGIYFNHASYNRLIGNVVFDNPVNGFGLYEFSNHNKIMNNTFSDNGDTGIN
                     IRESYDNQVANNTFVQDHVALHKPSPEYHTVANDNLFTDNIVSTEEERDAFVVTVLIF
                     DVSVFIVFLVFRKLLR"
     gene            6498..6836
                     /locus_tag="VN377_06520"
     CDS             6498..6836
                     /locus_tag="VN377_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWR28071.1"
                     /translation="MTKTTVILHSDSLGRGSDELGKTMTGAFLRKLWASAKKPDTIIF
                     YNSAVHLLTDKSLFLDALTGLSKAGVDLVACQTCIGFFEIEKKIVVGRIVDMTEIVSI
                     LMKSDTVITP"
     gene            6974..>10220
                     /locus_tag="VN377_06525"
     CDS             6974..>10220
                     /locus_tag="VN377_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28072.1"
                     /translation="MVSTNQREFLRVAVALLLLCSIVLPMASADEYLETNIDTLPYKQ
                     EITVPLDTSKEIAKYQPIDMRISFDNPCWGINETIHSIRVGVDDGTGLQEIESQIYDL
                     ESSDETHVKACSLVFLIPQEATGKEKYYVLYDSKETDPVNYPNHVTVEDTHYFYEPIP
                     GQKIDFDYYGIRQDGFVIYAVIQKGQLLGNPIALSAIKFKPNSTTVETFNLDQLGNFD
                     FRYGVPGEPDYVGTSWATEITKTVLVQGNLMVRVRLECTAPRGDIRSDNIYSYYYSPT
                     NAKRILIDAHHETLKSITIDDPTIMDGVYTGITSIKSRSASIEKMNVGEILPTVYVFT
                     EENAIQEYSVPQNPESTEREQVLSTQDDVELGSKAWACLGDATSGKVHGIIFSSNTGI
                     TEGSDDGVQIKVFAKQNIKLPGLEADTGNLYLTRNAFENGNHQTTLDQGKIYQYKVEF
                     LTVETGGYTRVDEESTFYQSLIKNIPVFRGNVTEGEGQKERYTLTVYAHFARSVPLGS
                     LFSAALGKNISYIYAELYQMNSFRSSGSVGRLSLGSISLNMTGKNLREKLQMVLGIFD
                     WKNLSLFKKIRFPDLDPGNYLVKIYRENPRFAKDHQYIGFAIVNLTKDSAIRIRCRPQ
                     GAIAIALTNQEKQGVENVRCVLEIDNTTIAEAVSDPEGNARLFAPCYSLKPYQLKIFY
                     QGFLIGEEQVRLNGLRRIIDMKKSFSLEQYGLSLTVTDTWGFPPAIDVNPTLVSDEMA
                     TATSIRAEPNNGGTYHFSNLLPASYRLSLSYKAFRLEQDVTLDQETTVTMMFPAEFAL
                     DLSIFNSYADRLSSGEISLQRNGKTQSTPIQKNGTTTAMIPPGDYEILVHANNEEIAQ
                     QQIQVRGEKTMDLITSQGSFVHTMILYLGLLFLIGMIGFLLWKKKLSTGIKLITISLL
                     IIALVSPWWALNGGTDTVSTTTNTMVIPPKLITLTKSSNATGGEISAVPAEVTMVLGL
                     LSMLVGAACLLIFFSVLTERRLRKTTLVLSVLSIVVILLTLIVFYYAFSQLTQVGVGS
                     FMGSGTLDVTIPGHSMQTAIPCAWGPGIGFYLLIVSFVLLLLTFFMKRIKAR"
BASE COUNT         3024 a         2293 c         2211 g         2692 t
ORIGIN      
        1 gcataatgcc atcccccgag aagcctacgc gaaattggtt cttgataaga aaaacaaagg
       61 ctcctttgtc tctgaaatca aggcagaaga aaaggaaatc tatgacgaaa taaaaccgat
      121 cgatccgaac ctcaaagtcg atgtatcatc ttgtgaatct tcgaagacgg cgatggaaaa
      181 atcctcacag gtgaagcttc tgaatctctt acagggactg ccccatggcg tccatcagat
      241 gaactacgat atcaagacac tggtcaacac ctcgacgaat cttgcgacgg tctctgtgaa
      301 agaaaatacg attgtgatcg ggatgagctc tcgtagtccg atgaaatccg cacttcagga
      361 catgcgggac cggatcaaag cgatcgcgtc tctttctgga gcgaaggtct ctgaggggac
      421 gccctatcct ggttggaaac ctgacctgca atcaaagatc ctagcgctat caaagaaaac
      481 attcaaagac atgttcaaaa ctgacgcgaa gatcgaggct atccatgctg gacttgaatg
      541 tggcatcatt ggtgaaaaat ttcctggtat ggatatgatc tccatcgggc cgaccttgaa
      601 gaacccacat tcccctgagg aacaactcca tatcagcacg gtggataaat tctatcgata
      661 tctgctgaag atccttgagt cagtctaagg gaacattgtg ccgccaagat gttcgttttc
      721 gatcatcatt cccgtactcc atgaatcaaa aatcattaat acactcctcg attcacttga
      781 tcgactgaag agggatgagc cttttgagat catcgtcgtc gatggaagcc cgacacagga
      841 taccctccag gtgatttcta agaaaaacgt gatgaaatac gtaagcccgc agggcagggg
      901 acgccagatg aacgaagggg cagcccattc gacaggagac atcctcatct ttcttcatgc
      961 agataccttt ctgccatcca acgcgcttac gctgatacag accactttgg cagaccagcg
     1021 ctatgtcggg ggtgcttttg ccttaggtat tcagtctcaa aaccctttgc ttaaaatgat
     1081 cgcatctttt tctacgctcc gatcgcgcct gacccgtgca ccatatggag atcaggtgat
     1141 tttccttcga aaatcctatt ttgacgctat cggaggatat cgggatattc cactcatgga
     1201 ggatgtggaa ctcatgcgtc gggtcaaaaa aaaggacgga gagattatca tcctctcaac
     1261 ctctgttatc acctctgacc ggagatggaa ccaagaagga ttactgtaca caacggtacg
     1321 agatttaatc atcatttttt tgtactggtg tgggatacct gccgaaaaac tcgtaaaatt
     1381 ctatccctgg cagaaagaat aaatccaaaa aatataggag aagaaaccgt tttttacata
     1441 caaattgata aaaagggata gtgaattcca aacacagaaa tcgacatccc ggggagtgta
     1501 atgtccgaga agaaaaacga gtttgaaaag cgtattcacg atgttaccca tgatggatta
     1561 cagtgccttg atcttcgtac cattcaaata aaccttggat tgctctgcaa ccaaacctgt
     1621 gaacattgtc atgtaaatgg ttcaccgaag agaacagaac aaatgagctg gccaaccatg
     1681 cagacaattt tatccatgac cgaaacacta tcaccattac tcattgatat cacgggggga
     1741 gcaccagagc tccatccgca tctcgaaaaa ttccttagaa gccttgcaaa acaacgtcat
     1801 cggattcaac tgagaacgaa ccttactatt cttttagatc cagcatataa gcatttcata
     1861 tccctctaca aagaattagg catagaactg gtcgcatcat tcccctgcta tctagaaaag
     1921 gaagtccgac tgcagcgtgg aaaaggcgtg tttgaaaaaa gcatcgaagc actccaactg
     1981 ctaaacaaaa tcggatatgg aacaacaccg gaccttccac tcatattagt gttcaacccc
     2041 cttgaacctg tgttaccacc ggaacaaaaa actcttgaaa acgagtaccg tatttatctc
     2101 gcagagcact acaaaataca gttcactaga ctcatcacgc ttacgaacat gccgcttggg
     2161 cgattcctca atgtattaaa agagaaaaaa ccacagtacc tgcgtttgtt gaaagaatca
     2221 ttcaacccag agactctgcc ttcactgatg tgccgtcatc agatcaatat cgcatgggac
     2281 ggtgccctct acgactgcga ttttaatctc gctgctggca tccccgtaac ctcagcggta
     2341 ccacgccata tccaacaatt taaaaaatct gcgatatcca ctcgaaaaat agcgaccgca
     2401 gaccattgct ttgggtgtac cgccggccag ggatcctcct gtggaggcgc tctcgtataa
     2461 cgaaaggaac gcacgtgaga tatgaaagaa agcaatcaaa aaaatgattg gtcgaaaggc
     2521 tggtggaaac cagttgtcct catcatcgtt atcgtcgcac tctttatcgt catgaatctg
     2581 tatgggtttg gacagaacat cacggaggta caacactgga tacaatcact cggtatctgg
     2641 gggccagttg cgttcttcat catctatctt ggatcggtga ttgcgaccat tcccgggacg
     2701 attttcgggg tgatcgctgg tggccttttt ggctctgtaa tcggagtgat tctcataagt
     2761 atctcatcga ctgttggcgc aagcatttgt ttccttatcg cccggtattt tgctcgtgat
     2821 actgtcgctc gttggttttc aaaaaataag accatgaaac gactcgatga actcacccaa
     2881 gaccagggta tgatgatcgt cggattaacc agattaatcc cgttatttcc cttcacgtta
     2941 ctgaattacg ggtttggtct aaccaaggtc tccttcaaaa cctatgtgtt ctggtcatgg
     3001 ctctgtatgc tccctggtac gattatcgtg gttgtcggca ctgatgccct cacccaagga
     3061 ctcacccaag gacgggtccc atgggaatta ctctgtgttt taatcattac cataattgct
     3121 cttgggatac tcgtctggta ctcacgacga acactcaagg aaaaaagaaa aaacaaagag
     3181 gaacaagaaa aaaataaaca agaatagact catagaaaaa aaggtttgat actcatatga
     3241 acggacaaac cgaaaaaaac aaggaatcag taccaacaaa agtattcctc caaaggaagg
     3301 tttccgaata catcgaacgt tgcattgaat gtaatagatg catggacgtc tgtccggtga
     3361 cgaaggatat attctccatc catgagttga atgtggcatc acaagaagga caaagcgtcc
     3421 ctttgaagat taaggaattc gcttttcatt gcatgcaatg cgggcaatgt gttccagtct
     3481 gcccgaagaa catccgcaga gaccacatgg tccgttatat caaatataaa attagaggtg
     3541 aaaaaccctg gagctacaag cgatatcttc tcataaaagg accaaatcaa actggaataa
     3601 gaagaaccat ccagcagctg tttatcagat cgaaaaaact cacgaacaag gatcttgctt
     3661 atttcatgga gacaatcccg gaaaagaaag cagaggtgct cttttatcca ggttgttata
     3721 tttactcgac gaagacggta caacaaacac ttcgcttact caaacatatc ggctgttcct
     3781 atactgttct tggaggggtg actacctgtt gtggtacacc tcatctgctg cagggtgaat
     3841 ttgaccaagc tgatgattgt ctttccctac tctaccagaa gatcaaagcc tgcgatcctc
     3901 agataatact caccgcatgt gccgaatgtt tcgaggcagt tgagcagata aaaaaaaggt
     3961 atacgatgga tgttgaggtc cttagcgtta ctcagtacct gttgcgatat caggacaagt
     4021 tcccttcaaa aaaaatcaga gggaagataa cggtccatga ttcctgcagg ttccatgcac
     4081 aatcacctca aggagtagca gttcatgatg tagtatcacg cttcggtgaa cttgtcaaac
     4141 ctcccattga tcaaccatcc aactgctgtt atcaatggaa ccatggtagc gatacaagaa
     4201 atgtgttgcg ccgagcgaat taccttgcag ctgtaaaaaa caatgcacca actctcgcat
     4261 gtaattgtct gacctgctat gaggagttca aaaaaacaca taccgatacc gagatcatcg
     4321 atgctctcca gctttttgag gaagcactcc aaccatcaga gaccagggag taagaatgat
     4381 gacaaaggac caagacacct gcatcctgct cttcgtaaaa tatcctgaaa aaggaaaggt
     4441 gaaactcaga ctctccagag gtcttaatga agaaatcgtt caagagctct atcgatgttt
     4501 tgtacaggat accctgacaa cgattaaaaa aattgatgcc ccgttcttca tctgttttca
     4561 tcctccagaa gcacagagta aatttcaaag ctggcttgga tctacccttc ggtttctccc
     4621 tcaacaagga gaggatcttg gggagcggat gaaaaacagt ttcacagatg tgttaactaa
     4681 aggatttcag aaagttattc ttatgggaag tgacagccca gaccttccag aagattacat
     4741 taaacaggca ttcaccaccc tgcaaaccaa ggatgcagtg cttggcccta cagttgatgg
     4801 cgggtactat ctcatcgggt ttaaaacaac aacttttacc cctaatgtgt tcgaggaaat
     4861 ccattggagc agccctctgg ttttccagga gacaatgatg aaattaaaac aggcacagcg
     4921 ttctgttggt ctattgccgg tctggagcga catcgacacg ccctccgacc tacataacct
     4981 cgttcggagg acaaggaata catcatttaa atcctcaaac acgatgacat atatccatca
     5041 acatcaaatc caagtggaga gcaaccatga agaaaaatca ggaactaaac caaactgagg
     5101 tattcctcgc tgataaaaaa acagggttat ggtactccct ttatctttcc atagcgagcg
     5161 tgtcgctctt tgcctcgatg gtcgccctca ataccgcata tcaaggtttt atctggagct
     5221 actcgacaag gtacgtcatt aactctttta tattggcgtt cgccttgttc atttcgggag
     5281 ctttcctgat tgtcgcgtgt atgaagctgc taaaaaaaag accgtccgcc cgtctttttg
     5341 gatttgtagg catcggtttt ctaatcgcct attcactgtt catccttatc atcgatcggt
     5401 atatcgcata taccctggtc tatgtcctta tgttgctcat tctctctgtt gtgatcatgg
     5461 ctggagcagt gttcttctat aaaaaaagct tctaaacaaa cgtgaaaaac ctttcgatca
     5521 tcgtaataat tttctgaaca cgagaaatac aataaaaact gaaacgtcga atatcagaac
     5581 ggtcactaca aacgcgtctc gttcttcctc tgttgaaacg atattatcag taaacaaatt
     5641 gtcgtttgca accgtatgat actcagggga tggtttatgc aacgcgacat gatcttgaac
     5701 gaaggtattg ttcgccacct gattatcgta agattcacgg atgttgatgc cagtgtctcc
     5761 gttatcagaa aacgtgttat tcatgatttt gttgtgattc gaaaattcat acaatccgaa
     5821 tccattcacg ggattatcaa aaacaacatt cccaatgagc ctattgtatg acgcatggtt
     5881 aaaataaatc ccacagcgac cattgtgatg tatcgtgtta ttgacaatta gattatcgct
     5941 tgttgaatat cctagttgca tgccatagaa attatcggtc aagatgttat gtgaaatcgt
     6001 gttatgacct gagacgacgt agacgccagc aaacggaaac ttcagttcac tatgggtaat
     6061 cgtaaaacca gaaacagtac tgttgcctgc tgcaagtgtg ataacataat ctttgttgtc
     6121 tccatcgatg atcgtgttat gtgtatcatc tccaatgaga tggacgggtt gaatgattct
     6181 caggttctca tgatacgttc cagggtaaac atacacggtt cctcgggtgg tcgcgctgtc
     6241 caaagcattc tgtatctgtg taaaattccc cggtccgctc ccaccgacat atacaattgg
     6301 tgttgtgata tcttcaccgg ttgtcggtga aaggaaaaag gaaaaagtac acaagaaaag
     6361 aagaatcaca atcacaagga atcttgtttt tttcatatgc ctatacccac gatatgagga
     6421 gcggggacta aaagtttgtg gcacgcataa ggtttaagtt ctccatatgt tttacttgct
     6481 tgtcttgagg gaaaaccatg acgaaaacaa ccgtgatttt acatagtgac tcgctcggaa
     6541 ggggaagcga tgaattagga aaaacaatga ccggtgcttt tcttcgtaag ctctgggcgt
     6601 cggcaaaaaa accagatacg attatttttt ataactcagc ggttcatttg ctgaccgata
     6661 aatcattgtt tcttgatgcg ttgacagggc tttccaaggc aggggtggat cttgtcgctt
     6721 gtcaaacctg cattggtttt tttgagatcg aaaagaaaat cgttgtcggc cggatcgtcg
     6781 atatgactga gatcgtctcg attttgatga aatcagatac ggtgatcact ccttagacaa
     6841 gccacgctcc aaacaaaaaa aaagtgcagc actggtccgt tcgtctctgt tatttcctcc
     6901 gcggtttgat ctttttcccc catcttactt aaaaagaata tttatataaa taaatgataa
     6961 tactattata tctatggtat cgacaaatca gcgggaattt ctcagggtag cagtcgcttt
     7021 actactcctt tgttctattg tactacccat ggcatctgct gatgagtacc ttgaaacgaa
     7081 catcgatacc cttccatata aacaagaaat cacggttcct cttgatacga gcaaagaaat
     7141 cgcgaagtac cagccaatcg atatgagaat atctttcgat aatccctgct gggggataaa
     7201 cgagacgatc cattcgatcc gcgtaggggt agacgacgga acagggcttc aagagatcga
     7261 atcacaaatc tatgaccttg aatcctccga tgagacccat gtcaaagcct gcagccttgt
     7321 attcttaatc cctcaggaag caactggaaa agaaaaatac tatgtccttt atgattcaaa
     7381 agaaacagac ccagtaaatt acccaaacca tgtaacagtt gaagatactc attattttta
     7441 tgaaccaatc cctggtcaga aaatagattt tgattattat ggcataaggc aggatgggtt
     7501 tgtcatctat gcggtgatac agaaaggtca gctgcttggc aatcccatcg cgctttccgc
     7561 catcaaattc aaacccaatt ccaccactgt cgaaaccttt aacttggatc agttagggaa
     7621 ttttgatttc cgctatggag tcccaggtga acctgattat gtaggcacct catgggccac
     7681 ggagatcaca aaaacagtgc tggtgcaagg aaatctaatg gtccgggtga gattagaatg
     7741 caccgccccg cgtggcgata ttcgatcaga taacatttat tcgtattatt atagtccgac
     7801 gaatgcgaaa agaatattga tcgatgccca tcatgagacc ctgaaatcga tcaccattga
     7861 tgatccgacg attatggacg gcgtctatac tggtattact tctattaaat cacggagtgc
     7921 atcgatagag aaaatgaacg tgggggaaat actacccact gtatatgttt ttacagaaga
     7981 gaacgcaatc caagagtatt ctgtgcctca gaatcctgag agcaccgaac gcgagcaggt
     8041 cctgtcgacc caggacgacg tggaactggg ttcaaaggca tgggcctgtc tgggcgatgc
     8101 gacgtcaggg aaagtccatg ggatcatttt ttcttcgaac acaggaatta ctgaggggtc
     8161 agatgatggt gttcaaatta aagtttttgc aaaacagaat atcaaactac caggacttga
     8221 agctgataca ggaaatttgt atctcacacg aaacgccttt gaaaacggta accatcaaac
     8281 cacacttgac caaggaaaaa tctatcaata caaggtagag tttttgacgg tggaaacagg
     8341 ggggtatacc agggtcgatg aagaatccac cttctatcaa agtttgatta aaaatatccc
     8401 cgtgttccgg ggaaacgtca cagaggggga aggacaaaaa gaacggtata cgttaacagt
     8461 ctatgctcat tttgctcgat ctgtcccgtt aggctcgttg ttttccgcag ccttaggcaa
     8521 aaacatttcc tatatctacg ctgagctcta ccaaatgaac agttttcgtt cgtcaggctc
     8581 cgtgggtcgc ctttccctag ggtcaatttc actgaacatg acgggaaaaa acctcaggga
     8641 gaagcttcag atggtgcttg ggatttttga ttggaaaaac ctttcattat tcaaaaagat
     8701 acgatttcca gatcttgatc ctgggaatta cctcgttaaa atatatcggg agaacccaag
     8761 atttgccaaa gatcatcaat acattggttt tgccattgtg aatcttacaa aggacagtgc
     8821 tatcaggatc aggtgtcgac cacagggtgc catcgccatt gctttgacca atcaggaaaa
     8881 acaaggagta gagaacgtcc gatgtgtctt ggagatcgac aatacgacca ttgcagaggc
     8941 agtatcagat ccggaaggga acgcgcgtct cttcgccccg tgttattccc tgaaaccata
     9001 tcagttgaag atcttctatc aaggtttttt gattggagaa gaacaggtga ggttgaatgg
     9061 tttacgtcga atcatcgata tgaaaaaatc gttttccctc gagcagtacg gtctttctct
     9121 gactgtcact gacacctggg gttttccacc agctattgat gtcaatccga cgcttgtgag
     9181 cgacgagatg gctactgcga cctccattcg agcagaacca aacaatggag ggacctatca
     9241 tttcagtaat ctacttcctg catcgtaccg tctgagcctg agttataaag catttcgact
     9301 tgagcaagat gttacccttg accaagaaac aacggttacc atgatgtttc ctgcggagtt
     9361 tgcccttgat ctatcgattt ttaactccta tgcagaccgg ctatccagtg gtgagatttc
     9421 actgcagaga aacggaaaaa cccagtcgac tccgattcaa aaaaatggga ctacgacagc
     9481 aatgatacca ccgggagatt atgagatcct cgtccatgcg aataacgagg aaatcgcaca
     9541 gcagcagata caggtacgtg gagaaaaaac catggacctc atcacctcac aaggttcttt
     9601 tgtccatacc atgattctct atcttggcct tcttttcttg attggtatga taggattcct
     9661 gctctggaag aagaagctat ctactggtat aaaactaatc acgattagcc tcctaatcat
     9721 agctctggtg tcgccctggt gggcgttaaa tggagggacc gacacggtgt cgacaacaac
     9781 caataccatg gtcatccccc caaaattaat cacgctgacg aaatcatcga atgcgaccgg
     9841 gggagaaatc agcgcagtcc ctgcagaggt caccatggtg ttagggctat tatccatgtt
     9901 ggtgggtgcc gcctgtctat taatcttttt cagtgtcttg acggagagac gactgcggaa
     9961 aacgaccctg gtgttatcag tcctaagcat cgttgtgatt cttctaactc tgattgtttt
    10021 ctattatgct tttagccagc ttacgcaagt gggtgtcggg agtttcatgg ggagtggcac
    10081 ccttgatgtc accatcccgg ggcactcaat gcaaacagca attccgtgtg cttggggccc
    10141 aggcatcggt ttttatcttc taatagtatc gtttgttctc ctgctgttga cgttttttat
    10201 gaaaagaatc aaggctaggt
//
LOCUS       DATMXJ010000248         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_15590, whole genome shotgun sequence.
ACCESSION   DATMXJ010000248 DATMXJ010000000
VERSION     DATMXJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15590"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1104
                     /locus_tag="VN377_06530"
     CDS             169..1104
                     /locus_tag="VN377_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWR28073.1"
                     /translation="MFDEEKTTKAVEKISPSVVNIITKRIMGYDRFNTYEFQGAGSGV
                     IMNPEGYILTNNHVVEQTKKVDVYLCNGEKYEGTVIGTDPSTDIAVIKINGDNFYSGE
                     FGNSDTLRPGQMAIAIGNSLGLSGGPSVTIGVISAIRRNLPSERGVLENMIQTDAAIN
                     PGNSGGPLIDSDGNIIGINNAIIPFAHGIGFAIPINVAKDVANELITFGRVTRPWLGI
                     LGVDINPTLASYYQLASNEGALIVQINEESPADKAGLKPGDIIIEIEGKLMKGMEDVR
                     HTIWKRKAGENITLKILRNHQQIRGTLKLAQNPAR"
     gene            1170..2258
                     /locus_tag="VN377_06535"
     CDS             1170..2258
                     /locus_tag="VN377_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWR28074.1"
                     /translation="MRSDAPSKPCLVSIQKSTYDTMDIRTLLEPIGGMTRYIKKGERV
                     LLKTNLLIPSEPKKAVVTHPAVIQALAEMILKSGAVPFIGDSPSGQFTKRRLEKAYQK
                     SGLTTIAKNLGIELNYDVGVTKVEIPLGTKLKKTPICDYVLHAEKIIAVPKIKTHSYM
                     IMTLATKIMYGSVPGLTKAKYHSLFIRRTAFADMLLDVLSIKKPDLIVMDGVIGMQGE
                     GPSGGAPVNLGVLLASKDAVGMDLSVCDMLSIEPIRIPILRQAKIRHLWPSEIQYPLL
                     SPQDVRYSDFRLPSSADSLFSTTKNHRQRPIPTEKCTACGQCVEICPKQAIQIRGHRA
                     SVDYTKCITCYCCHEICPYEAIKLDYLK"
     gene            complement(2297..>3096)
                     /locus_tag="VN377_06540"
     CDS             complement(2297..>3096)
                     /locus_tag="VN377_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR28075.1"
                     /translation="TNVTVFQRGNRLIPNAEPEISDLLKKQLEKRMQIHLNTEVLEVQ
                     KQGTTVTVIGNEGVSGREITVSAEELFVAAGRKSNADLLKVENTGVQTNSQGYILVNE
                     YLETSKKNIWAFGDAIGKAMFKHVANEEATIAWRNAFHEKKTKMDYQVIPYAVFTYPE
                     IAAVGSTEAEAKKHHQILVGTARYTDVAKGDAMMEYDGFAKAIVDKQTGKILGFHIIG
                     PYAPMLIQEVINGMALGGNIAFISQGMHIHPALPELILRTLGNLKEV"
BASE COUNT          935 a          730 c          628 g          803 t
ORIGIN      
        1 tcttaaggaa ttttctctga ttccctgatt ttatcattac cgttatggtc tttcttttca
       61 gggtaaatga cttacgatga gtgtttcttt ctatagaaaa gttaattaac aattgttaat
      121 aatatagttt gtacaaaaag aaaattaaaa aatgggggat cgaaataaat gtttgacgaa
      181 gaaaaaacca ccaaagcagt agaaaaaata agccccagtg tcgttaacat cattaccaaa
      241 cgaatcatgg ggtatgaccg attcaatacc tacgaattcc aaggagcagg ctcaggagtc
      301 atcatgaacc ctgagggata tatcctgacc aataatcatg tcgtagaaca aacaaaaaaa
      361 gtcgacgtct acctctgcaa cggagaaaaa tatgaaggaa ccgtcatcgg aactgaccca
      421 agtaccgaca tcgcagttat aaaaatcaac ggagacaact tctactcggg agaatttggg
      481 aactctgaca cattacgacc cggacagatg gcgatagcga taggaaattc actgggatta
      541 tcaggaggtc cttccgtgac aatcggagtg atctcagcga taagacggaa cctcccttca
      601 gaacgaggag tactcgaaaa catgattcag actgatgcag caatcaatcc aggcaacagc
      661 ggcggccctc tcattgactc cgacggtaac atcatcggta tcaacaacgc aatcattcct
      721 tttgcccatg gcatcggatt tgccatcccc atcaacgtgg caaaagacgt tgccaacgaa
      781 ctgatcacct tcggccgtgt cactagacca tggctcggta tccttggggt ggacatcaac
      841 ccaacccttg cctcctatta ccaactcgca tcaaatgaag gcgcgcttat cgtccagatc
      901 aacgaagaaa gcccggcgga caaagcagga ttaaaaccag gagacatcat catagaaatc
      961 gaaggaaaac ttatgaaagg aatggaagat gttcgccaca ccatctggaa acgaaaagcc
     1021 ggggaaaaca ttacccttaa aatactcagg aaccatcaac agatccgagg aacattgaaa
     1081 cttgcgcaaa atccagcaag gtaaaagaaa tcaaacacaa tattcgtact tttcacacaa
     1141 tacacacaaa acggagttga aatcatcaca tgagatctga tgcaccatca aaaccatgtc
     1201 tcgtcagtat ccaaaaaagc acctatgaca ccatggatat ccgtacgctt ttggaaccca
     1261 ttggtggaat gacacgctac atcaagaaag gggagcgggt cttgcttaaa accaatcttc
     1321 tcatccccag cgaaccaaaa aaagcggtcg tcacccaccc agcagtgatc caagcacttg
     1381 ctgaaatgat cttaaagagt ggagcagttc catttatcgg tgattccccc tcaggacaat
     1441 tcacgaaaag acgacttgaa aaagcatatc agaaatcagg gttaaccact attgcaaaaa
     1501 atcttggaat cgaactgaac tatgacgtcg gtgtcaccaa agtagaaatc cctctaggaa
     1561 caaaattgaa aaaaactcct atttgcgatt atgttttaca tgcggagaag atcatcgctg
     1621 ttccgaagat taagacacat tcctatatga ttatgaccct tgcaaccaaa atcatgtatg
     1681 gtagtgttcc aggcttgaca aaagcaaaat accattccct tttcatcaga cgaaccgcgt
     1741 ttgccgacat gcttctggat gtgctctcca tcaaaaaacc agatctcatc gtcatggatg
     1801 gtgtgatcgg aatgcaggga gaagggccat caggtggggc acctgttaat cttggggtac
     1861 tcctagcatc taaggatgca gtaggaatgg atctctcagt ctgtgacatg ctaagtatag
     1921 aaccgatacg catcccaatc cttcgacaag caaagataag acacctctgg ccatccgaga
     1981 tacaatatcc cctgctttcc cctcaggatg ttcgatactc cgattttcgt ttaccatcga
     2041 gtgctgactc ccttttctct acgacgaaga atcatcgtca acgaccaata ccgacagaga
     2101 aatgcaccgc ttgtggccaa tgtgttgaaa tctgtccaaa gcaggccatt caaatcagag
     2161 gacatcgcgc atcagtggat tatacaaaat gtatcacatg ttactgttgt catgagatat
     2221 gtccttacga ggcaataaaa ctcgactatt tgaaataaaa aaaatatatt ttttgttaaa
     2281 ctataggtgt gaatcctcac acttctttga gattccctaa tgtcctcagg atcaattcgg
     2341 ggagtgcagg atggatatgc atcccctgtg agatgaacgc gatgtttcca cccagcgcca
     2401 tcccattgat gacttcctga ataagcattg gtgcataggg tccaatgata tggaacccta
     2461 ggatttttcc ggtttgctta tcaacgattg ctttcgcaaa cccgtcatat tccatcatcg
     2521 catccccctt tgcaacatca gtatatcgag cagtgccaac gaggatttga tgatgctttt
     2581 ttgcttctgc ttcagtcgat ccaaccgcag cgatttccgg ataggtaaag actgcgtacg
     2641 ggatcacctg ataatccatt tttgttttct tctcatggaa cgcgtttctc caggcaatcg
     2701 ttgcctcctc gtttgccaca tgtttgaaca tcgcttttcc aatcgcatct ccaaaagccc
     2761 atatgttctt cttacttgtt tcgagatatt cgttaacgag gatgtatcct tgtgaattgg
     2821 tttgaacacc cgtgttttca actttcaaca gatcagcgtt tgattttcgg cctgctgcaa
     2881 cgaagagttc ctcagcagaa accgtaattt cccttccaga cacgccttca tttccgatta
     2941 cggtgactgt cgttccttgt ttttgtactt caagcacttc ggtgttcagg tggatttgca
     3001 tccgtttctc caattgtttt ttcaacagat ctgagatttc gggttcggca ttcgggatca
     3061 atcggtttcc tcgttggaag acggtgacgt tggtcc
//
LOCUS       DATMXJ010000249         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_5108, whole genome shotgun sequence.
ACCESSION   DATMXJ010000249 DATMXJ010000000
VERSION     DATMXJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5108"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1173)
                     /locus_tag="VN377_06545"
     CDS             complement(310..1173)
                     /locus_tag="VN377_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWR28076.1"
                     /translation="MRKKIIAILICMLFGTTSVLIIEAKSMISEENLIKSDVVINPTQ
                     AVGYSNITVAQAWDFLTNTSNGIQIPIDVRYDSEWIVAHIDTPYPENAQHWPSLQNGE
                     NLSEFMDLYQGKEIILYCKTGGRSKAAANLLVENNFDGIIYNMVGGIDAWIVAGYPTK
                     ANSPPGIPTINGENKGKVGEEYQYTMNTTDIDQDDIYYYVNWSDNTPDQLAGPYDSGE
                     AAVLNHIWSEKGTYIVKVKARDKYNAESDWANLTVTMPYSYDKPLLQFLGLLFERFPN
                     SFPLLRHLLGY"
     gene            complement(1427..2653)
                     /locus_tag="VN377_06550"
     CDS             complement(1427..2653)
                     /locus_tag="VN377_06550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWR28077.1"
                     /translation="MNKKLVCIILVGMFLGVVAHGSITNGTLEETPSIPNDQQQLRSD
                     FFFNTYIQTLMRLAHKPSLATCIISGDEMVWSHAYGYYDIENKKEATTQTLYLQASVS
                     KTITATALMQLYEQGLFDLDDDVNEFLPFSLRNPNYPDIPITIEMILSHRSSLADDNL
                     YWLCLSYLPGDPDVEGFPYPWLGEYLTPDGSAYSSSTWSLDKPGETYYYANIGFSIVA
                     YLVEILSHQDFNEYCKEHIFQPLQMTMTGFRLRDVDIDNVAVPYEFKNGGYFRHPQYG
                     IHVLYPAITLRTSIEDYSHFIIAHMNGGVWKGIRILNESTVELMHTAHFSPGDTYNYG
                     LGWEVTNKPFKKTYGHSGGYVGALNLVTIVPADNTAVILFSNALDSELHSTRMEWKAI
                     SAVNNALFQKAKKIAS"
     gene            complement(2834..4738)
                     /locus_tag="VN377_06555"
     CDS             complement(2834..4738)
                     /locus_tag="VN377_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR28078.1"
                     /translation="MKKILIIGIIGMIILSGLGTMAFATNTSFESTILTKNESTSVLF
                     SSPPIQVEQDGFMKIELIGATTQLLESNKPVVPIYIKTYEIPFGSSNIQVTCTPKDIN
                     TIFLTKDIIPASITPVSKMTEKTQYVKDSSVYESALFYPENWYNYELGAGRNQNDRQV
                     TFVKVICHPVRYAPLKNQVDYTVGFDITVTYMTPVAPKTVDTSYDMVIIAPAAFETTL
                     QPLIDFKNSKGVATTFKSMEDILAEYPGADQPEQVKYFIKDAYDTWNITYVLLTGGLK
                     NHIYAKDKDTISAGWKAWWVPVRYVSIPQDEDEGCLCDLYFGCLYNATGGFDSWDSNG
                     DGVYAAWGMGGYEKDTFDMYPEVYVSRVPVANTRELNRMVQKIITYESTGLAEKSWYN
                     TFVGVGGRTFSYYQGKPDGEYLCELAYNYTKLAIPDLKLVKVFTTNRDIGGLVPDKKG
                     IAKAFNQGAGFVDFEGHGGPSRWNTNWFDVDERVGITILNFARMQNGDKQPVVVVGGC
                     HNGMYNITALAAMLDKNGTTHFGYGYPVYICFSWGLVIKPFGGAIASTGCTGYGLGSG
                     SNPVSLSGELEMNFFYEIGNGSTNLAQAHSRAIQKYIAEESITSSDAFCITNWAVFGD
                     PSLRFGGYSS"
BASE COUNT         1416 a         1033 c          917 g         1510 t
ORIGIN      
        1 gtttttatac tgttattcaa ttattattaa gatatgttaa tataaagtaa tgacaataga
       61 ttacgtaagg tgtgatacta tggattacgt cctcgttgat gacagaactg gaaaacttaa
      121 gcttatctgg gaagagattt aaaccaattt actgaggttg agcagtatat tctttttaac
      181 tcctcctgcc tccccctctg cttaacctcc cctatcaatt attttaatac cgttctggca
      241 taggagaaaa attaaaatga tttgaaaata caagtttttt cggattaaag aaaaaaaaat
      301 tgggattttt tagtatccca acagatgccg taataatgga aacgaattag ggaaccgctc
      361 gaacaacaac ccaaggaact gtaatagcgg tttgtcatac gagtatggca ttgttactgt
      421 taaattagcc cagtcgctct ctgcgttata tttatcccta gcttttactt ttactatgta
      481 tgtccctttt tcagaccata tatggttcag tactgctgcc tcccctgaat cataagggcc
      541 agcaagttga tcgggtgtat tatcactcca atttacataa taatagatat catcttgatc
      601 gatatctgta gtattcatcg tatactgata ttcttcccca acttttcctt tattttctcc
      661 gttaattgtt ggaatacccg gaggtgagtt cgcttttgtt ggatatccag caacaatcca
      721 tgcgtcaata ccaccgacca tgttataaat aattccatcg aaattatttt caacaagaag
      781 gtttgcagca gctttgcttc taccacctgt tttacagtaa aggataattt ctttaccctg
      841 atataaatcc atgaattcac ttaggttttc tccattttgt aaactaggcc aatgttgtgc
      901 attttctgga taaggcgtgt cgatatgtgc cacaatccat tcggaatcat atcttacatc
      961 gattggtatc tgtatgccat ttgaagtatt agtaagaaag tcccaagctt gtgcaacagt
     1021 aatattactg taaccaacag cttgagttgg attgattacc acatcagatt ttatcaaatt
     1081 ttcttcagat atcatagatt ttgcttctat tatcaataca gatgtcgtac caaatagcat
     1141 acaaatcaag atggcgataa tttttttcct cattttattt ctccacaaag ctaaaattat
     1201 ttaaattaaa ttaataccat tcagttttta taagtttcta ttgtattgga agatacaatc
     1261 aacatagagt ctaatataaa gcgattccaa ccaaatttct cctttaaaaa catatcctta
     1321 gaaggagaaa tttatacggt ttttaagact caaggttttg aacaaaacaa taataaaata
     1381 atcgttcctt tcatttcttc tttcattttc cttattactg atttctttag gatgcgatct
     1441 tctttgcttt ctggaacaga gcattgttta cggcactgat tgctttccat tccatccgtg
     1501 ttgaatgtaa ttcagagtcc agtgcattgg aaaagagaat tactgccgtg ttgtcagctg
     1561 gaacaatcgt gacaaggttc agggcaccaa catacccgcc gctatgacca taggttttct
     1621 taaacggctt attggtaacc tcccagccca gtccgtaatt atatgtgtca ccaggtgaga
     1681 aatgcgcggt atgcatcaac tccacggttg attcattcag gattcgtatc cctttccata
     1741 ccccgccatt catatgggca atgatgaaat gagaatagtc ctcaattgat gtccgtaggg
     1801 taatcgcagg atacagcaca tggatcccgt actgcgggtg tctgaaatac cccccgtttt
     1861 taaattcata ggggactgct acattatcga tatcaacatc ccgtaaacga aacccggtca
     1921 tggtcatttg cagcggttgg aagatatgtt ccttacagta ttcgttgaaa tcctgatgcg
     1981 acagaatttc cacaagataa gcgacgatgg aaaatccgat attcgcatag taatatgttt
     2041 ccccaggctt atccagggac catgtcgagg agctataggc actaccatca ggtgtcaaat
     2101 attcccccag ccacggatac ggaaatcctt ccacatctgg gtccccaggc aggtaggaaa
     2161 gacaaagcca gtacagatta tcatcagcaa gacttgaccg gtgagacaag atcatctcta
     2221 ttgtgattgg gatatcaggg taattcgggt tcctcagact gaacgggagg aactcattga
     2281 catcatcatc caggtcaaaa agtccctgtt catacaactg catcaatgca gtggcagtga
     2341 tggttttcga taccgatgct tgaagatata acgtctgggt ggttgcttct tttttatttt
     2401 cgatatcgta gtatccatag gcatgtgacc agaccatctc atcaccagaa atgatacatg
     2461 tcgctaggga tggtttatgt gccagacgca taagtgtctg tatataggtg ttaaagaaaa
     2521 aatcagaacg cagttgttgc tggtcatttg gaatggacgg ggtttcttca agagtcccat
     2581 ttgtgatact cccatgggca acgactccaa ggaacatacc tacgagtatt atacatacga
     2641 gtttcttatt catatgattc accaatgtcc gattcattat catatatttt tgttgttata
     2701 taaaagtaat ctctcagtta tttaaacgta aggaaaagga aaaaaataga taacagcagt
     2761 attttactgt gaaaaactca aggatttttc attcgaaata gatgtaaaaa gtaaaaagga
     2821 agatatttct caactatgag gaatatccac caaacctcag acttggatcc ccaaagacgg
     2881 cccagtttgt tatacagaac gcatcggagg acgtgatact ttcttcagcg atgtacttct
     2941 gaatcgcacg gctatgcgcc tgggcaagat tcgtcgaccc attgcctatc tcataaaaga
     3001 agttcatctc aagttctcca ctgagactta cggggttcga ccctgaacca agaccatacc
     3061 cagtacatcc ggtagaggcg atcgcgccac caaacggctt tatcaccaaa ccccagctga
     3121 aacagatata cacaggatat ccatagccga agtgggttgt tccattttta tcaagcatag
     3181 cagccagtgc cgtgatatta tacatcccgt tatggcaccc accgacgacg accactggtt
     3241 gcttgtctcc gttttgcatt cgtgcgaaat tcaaaatcgt gatcccaacc cgttcatcga
     3301 catcaaacca gttcgtattc catcgggatg gaccgccatg tccttcgaag tcgacaaagc
     3361 cagctccttg attaaatgcc ttggcgatgc ctttcttgtc agggaccaga ccaccgatat
     3421 ctctgttcgt ggtgaacact tttactaact tgagatctgg gatggcaagt tttgtataat
     3481 tataggcaag ttcacagagg tattcgccat caggtttccc ttgataatac gaaaaggttc
     3541 ttcccccgac tccgacaaag gtgttgtacc atgatttttc cgctagacct gtgctctcgt
     3601 aggtgatgat tttttgtacc atacggttga gttctcttgt attggctact ggcactcggc
     3661 tcacatagac ttcagggtac atatcgaagg tatctttttc ataacccccc atgccccaag
     3721 cggcatagac gccatcacca ttcgaatccc aactgtcaaa accaccagtt gcattataca
     3781 gacatccgaa gtagaggtca cagagacatc cttcatcctc gtcctgtggg atgctgacgt
     3841 aacggactgg gacccaccat gctttccaac ccgcagagat ggtgtcctta tcttttgcat
     3901 agatatgatt tttcaaacca cccgtaagta agacataggt gatattccag gtatcgtacg
     3961 catctttaat gaaatatttc acctgttccg gttggtctgc tccagggtat tcagcaagga
     4021 tatcttccat cgatttaaag gtcgttgcaa cccctttgct gttcttaaaa tcaatcaaag
     4081 gttgaagggt cgtttcaaac gcagctgggg caataatcac catatcatag gaggtatcaa
     4141 ccgtttttgg tgctactggt gtcatatagg tgacggtgat gtcaaatccc actgtataat
     4201 caacctgatt ctttaacggt gcatatcgaa cagggtgaca gatgactttt acaaaggtta
     4261 cttgcctatc attctgattc cggcctgctc caagttcgta gttgtaccag ttctcaggat
     4321 aaaacaaagc gctttcatag acagaggaat ccttcacata ctgtgtcttc tcagtcattt
     4381 tcgatactgg agtgatagac gcagggataa tatctttagt aagaaatata gtgttgatat
     4441 ccttgggtgt acacgttacc tgaatattgg atgatccaaa aggaatctca tacgtcttga
     4501 tataaatagg tacgactggt ttatttgatt cgagtaattg tgttgtcgca ccaatgagct
     4561 caattttcat aaaaccatct tgttcaactt gtatcggtgg tgatgaaaaa agcactgatg
     4621 tcgactcatt tttcgtaaga atcgttgatt caaaagaggt atttgtggca aacgccatgg
     4681 tccctaaccc actcaaaata atcattccta ttattcctat gatcaatatt tttttcatat
     4741 atttcattcc ctctaatgca atattaatcc atttctaata tttaattact ttcctcaaag
     4801 aaaataaagt acaatacgaa aaatgaacca tcagatttat ttctccttat tatagtatac
     4861 gtaactgtta tcttga
//
LOCUS       DATMXJ010000250         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36492, whole genome shotgun sequence.
ACCESSION   DATMXJ010000250 DATMXJ010000000
VERSION     DATMXJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36492"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..509)
                     /locus_tag="VN377_06560"
     CDS             complement(78..509)
                     /locus_tag="VN377_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28079.1"
                     /translation="METSTPSETSINDYYPDQNQQPSQPSSKPLIAGIFLIIAGLLGL
                     FTWASAMALDISMIQNVLPAESPITAEQLQSFLTTCGVIGAALSIFTLAGGIVAIKRK
                     AWGLAIIGGILGLFTIGPMLLGSVLSLIGLILVAISRKDFQ"
     gene            complement(540..1220)
                     /locus_tag="VN377_06565"
     CDS             complement(540..1220)
                     /locus_tag="VN377_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease PrsW"
                     /protein_id="HWR28080.1"
                     /translation="MNSFIPQTSLFIGIIPALIVLYIGLKGYEGHFKDKLIFLTFIVG
                     IIIGFISAVIELFTQYIGLIFYIMLFPILEQLFKTIVLNIGRFQEKQETTIYGLSLGL
                     GFGSVFTPAAIILGNFQEINLSFIAAIIGSFGIILFHAGTGVIIGYGVYTHKLPTYVT
                     MAILLHFVITTVIGVTNLLQVSYLQIILVIYGILIYWYATKRIMPRILNESQRRKRTQ
                     KEIDIKSK"
     gene            1557..>2351
                     /locus_tag="VN377_06570"
     CDS             1557..>2351
                     /locus_tag="VN377_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWR28081.1"
                     /translation="MVQRRYDFDVLRVFCTGSIFLFHCFCIFAAEDYFIVNPQTSVVL
                     YILVYLMLIWMLPVFFIISGRVTYYSLDSNTTLGLVKSRFLRYMVPFISGVLFLSPII
                     IYLSRVFRNEYSGSLLSFYFNNYFHGLYGFGGNFSFLGNHLFYLLYLFVFTMFFLLVV
                     RLKPWKRGQGKRHDWLALFQKPGAIFLLSIPIIITTELNFIDPTIFGRTGTGGWNIFS
                     HFLFFAYGLFFSFDHRFDNVIDRHWKISAVIAIPLLAMYAWLLTDVP"
BASE COUNT          606 a          449 c          492 g          804 t
ORIGIN      
        1 aattgcatgt gtatagatac aatatctttt cttctttgtt gaaaaagaga tttttttggg
       61 taatgttaac agtatcttta ttggaaatcc tttcttgaaa tcgccacgag gataagacca
      121 atgagtgaga gcacactacc aaggagcatg ggacctattg taaagagtcc aaggatccct
      181 ccgatgattg caagtcccca tgcttttcgt tttatggcga cgataccacc agcaagggta
      241 aagattgaca gggcagctcc gatgacacca caggttgtta gaaacgattg cagttgttct
      301 gctgtgatag gggattccgc gggaagcacg ttttgaatca tcgatatatc tagtgccatg
      361 gctgaagccc atgtgaataa tcctaataat cctgcgatga ttaaaaagat gcctgcaatg
      421 agtggtttgg aactaggttg acttggctgt tgattttgat ctggataata atcattaata
      481 gatgtttcac ttggggtcga agtttccatc tcttacacct ctatgctcta ttgtaatcgt
      541 tatttgcttt ttatgtctat ttctttttgc gtccgttttc gtcgttgact ttcgttaagt
      601 attcgtggca tgattctttt tgttgcatac cagtaaatta agatgccgta gatgacaagg
      661 attatttgta gatagctgac ttgtaatagg tttgtcactc ctatgactgt ggtgatgacg
      721 aaatgcaaaa ggatagccat ggtaacatac gtcggtaatt tatgggtata gacaccgtat
      781 ccgatgataa ctccggttcc agcatggaag aggatgattc caaaggatcc gatgattgct
      841 gcgatgaagc tgagatttat ctcctggaag ttccctagta tgatggctgc tggggtgaac
      901 actgacccaa acccaagtcc cagggaaaga ccatagatgg ttgtctcttg tttctcttga
      961 aatcgtccga tgttcaggac tattgtttta aaaagttgct caaggatggg aaacaacatt
     1021 atgtagaaga tgaggccgat gtattgtgtg aatagttcga ttaccgccga gatgaaaccg
     1081 ataatgatgc cgacaatgaa cgttaagaag atgagtttgt ctttgaaatg tccttcgtag
     1141 ccttttaatc ctatgtataa cacgattaat gcgggtatta tgccaatgaa gaggcttgtc
     1201 tgtgggatga aactgttcat ggttttctgc gggtttatga agatgtgata tattaagcat
     1261 ttctattgtc caaaaaatta tacatcaaat aagaattaca aatgtatata tctttaaata
     1321 tccttttaat ctgttagaag tgtccaaaac actcatcgcg taaaacgaaa tcgttttgga
     1381 ttcttatcaa caatgcagct gatttttcct taggtttctt gttttacgat gcaacctata
     1441 gggtgacacc tataatccct tgtcaaactg ataaataatt gtttgctctc cttaggagtt
     1501 cttcattctt cagggacgta agcattaata acatctttta tgttttcctc gatgaaatgg
     1561 ttcaacgacg ctatgatttt gacgtgctac gagtgttttg cacagggtcg attttcttat
     1621 ttcactgttt ctgtattttt gcagccgaag attactttat tgtaaatcct cagaccagtg
     1681 tagtattata catccttgtc tacttaatgc tgatatggat gcttccggtg ttcttcatca
     1741 tctctggacg ggtaacctac tattcgcttg acagtaacac taccctaggg ttggtaaaat
     1801 ctcgttttct cagatatatg gttccattta tatctggtgt tctttttctc tcgccgatta
     1861 tcatttatct cagtcgtgtt tttcgtaatg agtacagtgg aagcctgctc tcgttttatt
     1921 tcaataatta tttccatgga ctatatggat tcggcggtaa tttttcgttc ctgggaaacc
     1981 atctcttcta cctcctgtat ctcttcgttt ttactatgtt tttcctgctc gtggttcgtc
     2041 tcaaaccctg gaaaagaggt caaggaaaaa gacacgattg gttagcgttg ttccaaaaac
     2101 ccggggcgat tttcttacta agcatcccga ttattattac aacagagctg aatttcattg
     2161 atccgacgat ctttggtcgg actggtaccg gcggctggaa catcttttcg catttccttt
     2221 ttttcgcata cggtctcttc ttctcttttg accaccggtt tgataacgtt attgatcgtc
     2281 actggaagat ctccgcagtc atcgccatcc cactgcttgc gatgtacgcc tggttactaa
     2341 cagatgtacc t
//
LOCUS       DATMXJ010000251         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72442, whole genome shotgun sequence.
ACCESSION   DATMXJ010000251 DATMXJ010000000
VERSION     DATMXJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72442"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /gene="iorA"
                     /locus_tag="VN377_06575"
     CDS             complement(<1..1672)
                     /gene="iorA"
                     /locus_tag="VN377_06575"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HWR28082.1"
                     /translation="MLLFFYHIQGNCASFTSMVMMMSELTQEKPGKQILLLANEAITR
                     GALEAGIDVATTYPGTPSSEIADTFSAIAKEAIQQNKDPGFYFQYSVNEKVALEVAAA
                     ASFCGLRALTCMKHVGLNVASDTFMTYMYVGCRGGHIIVSADDPYCHSSQNEQDNRYY
                     ALFGGAPMLEPTTPQEAKEMTRIGFDISEKLSLPLLLRTTTRLNHARGPITLNKMQQP
                     RKKGFFKKDPLLVTVPATARAKHPMLLKQLEEAEKISEKSPFNEIIPIGKPKDAGIVT
                     SGVSYTYVKEMAEELNLNVKILKLGMTHPIPRAMCEKFITSCSSLIMVEELEPFLENQ
                     FKQLAYDIGADVKIYGKSSGHFSRLYEYTPDIVVDAFSKIFKVKNPFPGPTQSALKAP
                     RRPPSLCPGCPHRATYYAVKKAAPKDTIYPTDIGCYTLGKEPPLEIADVLLCMGSNAG
                     MACGFSVATDQKIISFMGDSTFFHSGIPPIINAVHHSHDVVITVLDNRTTAMTGHQPH
                     PGTPYDGMGRPAKLIKVEDVVKGCGIEHIEIVDPNKIKETTAAFKRAIE"
     gene            complement(1779..2027)
                     /locus_tag="VN377_06580"
     CDS             complement(1779..2027)
                     /locus_tag="VN377_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWR28083.1"
                     /translation="MLTIEYQETLLKTISKIKDQAVKEKIKKQIKRIVENPEIGKPMR
                     YTRKGTRELHIGSYRLAYAYFEEEKKLIFLDIYHKNEQ"
     gene            complement(2021..2287)
                     /locus_tag="VN377_06585"
     CDS             complement(2021..2287)
                     /locus_tag="VN377_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWR28084.1"
                     /translation="MDVAITKISSKGQIVIPSHLRKGISVGEEFLIIRDHDRIIMMKI
                     NELTKEFKEDLEFAQHVEKAWKTYEKTKFKSLSADKFLEELEKC"
BASE COUNT          787 a          623 c          723 g          975 t
ORIGIN      
        1 attctattgc tcgtttgaat gctgcggtgg tttctttgat tttgttggga tcgacaatct
       61 cgatatgctc gataccgcag cctttgacga cgtcttcgac tttgatgagt ttggctggtc
      121 gtcccatccc atcatagggg gtccctgggt gcggctgatg gccggtcatc gctgtggtac
      181 ggttatcaag gaccgtgatg acgacatcat ggctgtgatg tacggcatta ataatcggtg
      241 ggatcccaga atggaaaaac gttgagtctc ccatgaatga gataatcttc tgatcggttg
      301 cgacggagaa accgcaagcc atccctgcgt tcgatcccat gcagaggagc acatccgcta
      361 tctcgagtgg gggttctttt cctagtgtgt agcatccgat gtctgttgga tagatcgtgt
      421 cttttggtgc tgctttcttg accgcataat atgttgctcg atgcgggcag cctgggcaga
      481 gtgatggcgg gcgtcgtggg gctttcaaag cagattgagt tggtcctgga aacgggtttt
      541 tcactttgaa tatttttgag aacgcatcaa caacgatatc aggggtgtat tcatagagcc
      601 gggagaaatg accgctggat ttgccgtaga tcttcacatc agcaccgata tcgtacgcga
      661 gttgtttgaa ctggttctca aggaacggtt cgagctcttc gaccatgata agggacgagc
      721 aggatgtgat gaatttttcg cacatggcac gtgggatcgg atgggtcatc ccaagtttaa
      781 ggatcttgac attcaagttc aattcttctg ccatttcttt gacataggta taggagactc
      841 cggaggtgac gatcccagcg tcctttggtt tcccgattgg tattatttcg ttgaagggtg
      901 atttttctga gattttctct gcttcctcaa gttgtttgag gagcatcggg tgttttgctc
      961 gtgcggtcgc agggaccgtg acgagcaagg ggtctttctt gaagaaccct tttttgcgtg
     1021 gttgctgcat tttattaagt gtgataggac ctcgcgcatg gttcaaccgt gttgtcgtac
     1081 gaaggagcaa gggaagagag agtttctctg agatatcgaa cccgatccgt gtcatctcct
     1141 ttgcctcttg tggtgtggtc ggctcaagca tcggtgcacc accgaacaat gcgtagtacc
     1201 ggttgtcttg ctcgttttgc gatgaatgac agtacgggtc gtcagcggag acaatgatat
     1261 gcccgccgcg gcagcccacg tacatgtatg tcatgaatgt gtcgctggcg acattcagac
     1321 cgacatgttt catgcaggtc agtgctcgca gtccgcagaa cgatgcggca gcagcgacct
     1381 ctaaggctac tttttcattg accgagtatt ggaaatagaa accagggtct ttgttttgtt
     1441 ggattgcttc ttttgctata gctgaaaacg tatcagcaat ctccgatgat ggtgtaccgg
     1501 ggtaggtggt tgcgacatcg atgccagcct caagggcacc acgggtgatc gcttcatttg
     1561 ctaacagcag tatttgcttc cctggtttct cttgtgtgag ttcagacatc atcatcacca
     1621 tcgaggtaaa agatgcacaa ttcccctgga tgtggtaaaa aaagagcagc atataaatct
     1681 aaccccactc gggaaggtat ttatgataga ctcaaatggt gttatgatgt ggtgaggtgt
     1741 atgtacggag atttggagtt aggagctgat ggaatcatct attgttcatt tttatgatag
     1801 atatctagaa atattaactt tttttcttcc tcaaaatatg cgtatgctag tctataagaa
     1861 ccaatatgga gttctcgtgt tcctttcctt gtgtatcgca ttggttttcc aatttctgga
     1921 ttctcaacta ttctttttat ctgtttttta attttttctt tcaccgcttg atctttaatt
     1981 ttcgatattg tttttagtag agtttcttga tattcaatgg tcagcatttt tcaagttcct
     2041 ctagaaactt gtctgcagaa agagatttaa attttgtttt ttcataggtt ttccatgctt
     2101 tttctacatg ttgggcgaac tcgaggtcct ctttgaactc ttttgttagt tcattgatct
     2161 tcatcataat tattctatca tggtcccgaa taatcagaaa ctcttcacct acagaaatcc
     2221 cttttctgag atgcgatgga atcacgattt gtccttttga acttattttt gtaattgcta
     2281 cgtccattgt atcactaagg taagatatgt cttacatagt atttatagat tatgtttcca
     2341 aggataattc ttggaggtac caggagaact acctacttgt ggtggcgcac ctgcatcact
     2401 acttgcatat acaactacaa acaaggtact taaaacagtg gctctttcat tggtctggaa
     2461 ggaattaagc tggtagttgt agagtgcatc ccatccctcc tagtgagacc agcatccttc
     2521 ccttttttta tgaaaaaaga ttttttcttg ccatgacacg acatggtttt tttgcacaaa
     2581 tacgaaacta tatgtactca acgcctgtta cagaaaaact ggagagatta agaaggagtt
     2641 atcgggatca cttacacgac taccacaact gcttgtcatc ctgattctta gcacttcttg
     2701 actgtgaaaa agacactgga gggttggaga aatatgacca caaaaaatta cgtgataata
     2761 gaacataaga tagtcccgaa acaagtacca ctattcgaag aacaagacga tgggaacctg
     2821 ataccgtaca taaaggaagc ataacccatt ctttttttgt gagccacact gttttaccac
     2881 tggtttacca aaattaaaac ttatttttaa cgcgttcatt ttctgtaaaa ataaatagat
     2941 ctataatacc gttcatccat agatttcagg gcagaatcat tgaacaggca ttcaagaacg
     3001 ggtaccgcct gggagcattt atatatatta tgataattca atggaggcga ccgagagata
     3061 tggtaccgat aacaaaatca gaagaaaaaa tacagcgcta ctatgata
//
LOCUS       DATMXJ010000252         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72476, whole genome shotgun sequence.
ACCESSION   DATMXJ010000252 DATMXJ010000000
VERSION     DATMXJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72476"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3854
                     /locus_tag="VN377_06590"
     CDS             <1..3854
                     /locus_tag="VN377_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWR28085.1"
                     /translation="NDIYRLKYADKEYAERSSRGPFFSYVVQHFRFKVDIDPDAVDKL
                     YVLWTGTADASARVDFYFYNNSYFSYLKRGAWELLPDNIGSSGGSPYSLNNTIHGSAL
                     KYAIDGNNYIDICAVATHPLIRQLCTLSTDFISLVAVTEKGYSLVTAIAETTTDIVPS
                     NISTGAFNWDLLTWDDYQPTGSEARYQVLFMNATGKFVEVEESALPGNHKGFRTAPVY
                     LNNLSNYDYHTKYNKLKIRVNISTDSPSVSPRIFNWALTWQNKTRWQDSFETYYRIDS
                     RTKVNKVNGTIMLSSVQDEWPMFGFDAANTRASGGKAATSANLYWFGGEYVGGEFRNP
                     VIGNGNVYIFSNGRTLHQYPVILPSGAEEGAPRINTTSITLDQDVVNSPAVTDDLVIV
                     ATGQKAKEGSENFIYGYELNNLNRSWVYDYNQKICYDASPVIDGSWLFITTWGGDNKS
                     YVFESQRYTNNKLLALQINTESAKLESEYSLPAPSFSSPAVTSEKIIVTCSSTDNESV
                     IALSRNGGSIGEKIWGKTVGAVGHASPVIYEDTVFVTCAQVTDKKYVTKIVAMNINDG
                     TILWNKTLGTPSSSYSNVAESTPAVYNGVLYAASADGTVYALDTVNGTTLWSKEIYTV
                     PLFSDNILRSSPAYAENHVYIGTPGGLIYALDASTGNPLWDYETFPIWTAAPVLGSPA
                     VSNGLVFIADENGVLYSLGRFTTSTKQVSGRIISVPIRLPEALWWDKFYADNSSYNSS
                     SSISFKLLDENGNVLNNDLKNRSSLTQGGLVLGRAVRLQADFSSTNLSRSNPKLFRWY
                     LTLTSDTQKPFLNSSSFTPSSGGWLQEIVPVFTIKVKDNGTGLRVKSAVYTLEYTLNN
                     VSQRNTYPAVCTGDNGTNAWQTLTMNISKISNFDNITSLRSIMFNITDLAGNAASKTV
                     TFKQDKTKPTSLINKNGMSERYNSTNIQINATAKDNGTVNVDASGVKLVELYYRYSQN
                     RNFSGDWVYFSNSTKTSPMWLFNFTNRPSQHGGYFELCTIAYDYANNNESFPSTGDVW
                     FLYDWKEPELPSYSGDTLWFKERPQLSVVFEDDYRLDTIRYRPSFDTIWTTLASDVNM
                     SVYNTDAVGNTWVLSQADWDQMVEDEIYYLYFRINDTLGNTLEILNDDQAITIRKDTL
                     PPLVSIDVPSVEDEWSMSDNFTVSGLGSDHDGSGIKEASLYYRFSEDKSNWSSWTPFG
                     DMLDSSPFEWEFDATEGDGYYEMKIMATDYAGNEVESEVFPLAIASFPMTLALVLVGL
                     VTVLLLLSVIIYLKWRKKETA"
     gene            complement(3851..4039)
                     /locus_tag="VN377_06595"
     CDS             complement(3851..4039)
                     /locus_tag="VN377_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28086.1"
                     /translation="MQKKYVRICPVCNSTDVQPDLSADSYAKGLLNQWKCNSCGYTGL
                     FFPEYTAEDVKKIKEKNP"
     gene            complement(4053..>4803)
                     /locus_tag="VN377_06600"
     CDS             complement(4053..>4803)
                     /locus_tag="VN377_06600"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWR28087.1"
                     /translation="SMLQRHIQKILDAFGTWDLRVASNEIFFEIQKDLQWYLKRGGTD
                     KQLLTHYVHTWIILMTPITPHLTEELWTAQGYNQFVSNETYPEFKPQDISEKDEIGEY
                     LLTRVIEDVNEIVKVTKMTPKKICIYTSPTWKQNIYRKALEQSAQKTFNVGQLIKETT
                     ADPRMKPLGQQVSQFVAKIAPEVKMLSDTDRGRFLIAIDEKAHLSDAKPYLSEVLHCP
                     VEIYSADDLNLYDPAKKTRFASPLRPAIYIE"
BASE COUNT         1254 a         1069 c         1116 g         1364 t
ORIGIN      
        1 gtaacgacat ctaccgactc aaatatgctg acaaggaata cgcggaacga tccagccgag
       61 ggcccttctt cagctatgtc gttcaacatt ttcgctttaa agtcgatatc gacccagacg
      121 ctgtcgataa attgtatgtc ctttggactg gaaccgcaga tgcttcggct cgtgtcgatt
      181 tttatttcta taacaatagt tatttcagtt atttaaaacg cggtgcatgg gagttactac
      241 cggataacat tggctcctcc ggtggaagtc cctactcatt gaataatact atccatggta
      301 gtgctctgaa gtatgcaatc gacggaaaca attacatcga tatctgtgct gtcgcaaccc
      361 atccgttgat tcgccaatta tgcacattgt ccactgattt tatcagcctt gttgcagtca
      421 ctgagaaggg atactcattg gtaactgcta tagctgagac aacgacggac attgtgccgt
      481 cgaatatctc gacgggtgct tttaactggg atcttctgac ctgggatgat tatcagccaa
      541 caggctccga ggctcgctat caggtcttat tcatgaacgc gacagggaaa tttgtcgaag
      601 tagaagaatc cgcattacct gggaaccaca aaggattccg caccgcccct gtctatctga
      661 ataacctttc aaactatgat tatcatacga agtataataa attgaagatt cgcgtgaaca
      721 tttcaacgga ttctccatca gtctccccaa ggattttcaa ctgggcgttg acctggcaga
      781 acaaaacccg ctggcaagat tctttcgaaa cctattatcg gatcgattca cggaccaagg
      841 tcaacaaagt gaacggaaca atcatgctct cttcggtaca agatgaatgg ccgatgtttg
      901 gttttgatgc cgcaaacaca cgagcatccg gtgggaaagc cgcaacaagt gccaatctgt
      961 attggtttgg tggcgagtac gttggtgggg aattccgcaa ccctgtcatc gggaatggaa
     1021 atgtctatat tttttccaat ggacgcaccc tgcatcaata cccggtgatc ctcccaagcg
     1081 gtgccgagga aggtgcgcct cggatcaata caacctctat cacattagat caagatgtgg
     1141 tgaactctcc tgctgtgact gacgatctgg tgattgtcgc aaccggtcag aaagcaaaag
     1201 aaggaagtga aaatttcatc tatggatatg aactcaataa cctcaaccgt agttgggtat
     1261 atgattataa tcagaaaatc tgttatgatg catctcctgt tatcgatggg tcatggctat
     1321 tcattaccac ttggggtggt gacaacaaaa gctacgtctt cgaatcacaa cgatatacca
     1381 ataataagct tctagcacta caaattaata ctgaatccgc taaattggaa agcgaatatt
     1441 ccctacctgc tccaagtttc tcaagccctg cggtgacctc cgaaaagatc atagttacct
     1501 gtagttcaac tgacaacgag agtgtgattg ctcttagtcg taatggagga agcattggag
     1561 aaaaaatctg gggtaaaacg gttggagcgg tcggccatgc atccccagtc atctatgaag
     1621 ataccgtctt tgtcacctgt gctcaggtca ccgataaaaa atatgtgaca aagatcgtcg
     1681 caatgaacat caatgatggc accatcttat ggaataaaac gcttggtacg ccatcttcct
     1741 cttatagcaa tgtcgcagag tccacacccg cggtgtataa tggagtcctg tatgctgctt
     1801 ctgccgatgg gaccgtctat gcacttgata cggtgaatgg gacgacacta tggtctaaag
     1861 aaatctatac cgtgccgctc ttctctgata acatcctgcg gtcatctcca gcgtatgcag
     1921 aaaaccatgt gtatatcggc accccaggtg gtttgattta tgcattggat gcctcaacgg
     1981 gtaaccctct ttgggactat gaaacgttcc ctatatggac cgcagcacca gtcctcggat
     2041 caccagcagt atccaatgga ttagtattca ttgccgatga gaatggggtg ttgtacagtc
     2101 tgggtcggtt caccacgtct acaaaacaag tcagtggtcg tatcatatct gttcctatcc
     2161 gattacctga ggcgttatgg tgggataagt tttatgcgga taactcttct tataactcgt
     2221 ctagcagtat atcgtttaag ttgcttgatg aaaacggcaa tgtgctcaat aatgatttaa
     2281 aaaatagaag tagtctaaca caagggggtc tcgtacttgg tagagcagtg cgactccaag
     2341 ctgatttctc ctcaacaaat ctctcaagga gtaatccgaa gcttttccgg tggtatctca
     2401 cgcttacctc agatactcaa aaaccgttcc tcaacagtag tagttttacc ccaagctccg
     2461 ggggatggct ccaggagatc gtccctgtgt ttaccataaa ggtgaaggat aatgggaccg
     2521 ggttgcgcgt caaatccgca gtgtataccc ttgaatacac tctgaataat gtcagccaga
     2581 ggaacaccta tcctgcggta tgtactggag ataatgggac gaacgcctgg cagactctga
     2641 cgatgaacat ctcaaaaata tccaacttcg ataatatcac ttccttgcgt agcatcatgt
     2701 tcaacatcac tgatctggcg gggaatgctg cctcaaagac cgtgacattc aaacaggata
     2761 aaactaagcc aacatcactt atcaataaaa atggtatgag cgagagatat aactctacga
     2821 acatccaaat caatgcgaca gccaaagaca acgggaccgt gaatgtcgat gccagtggcg
     2881 tcaaactcgt tgagttgtat tatcggtact cgcagaatag gaacttttct ggggactggg
     2941 tttatttttc gaattcaacg aaaacatcgc cgatgtggtt gttcaacttc accaatcgtc
     3001 cgagtcaaca tggtgggtat ttcgaactat gcacaattgc gtatgattat gcgaataaca
     3061 atgagagttt cccctctacc ggtgatgtct ggttcttgta tgattggaaa gaacctgagc
     3121 tccccagtta ttctggtgat accctctggt tcaaggaacg accccagttg tccgtggtgt
     3181 tcgaggatga ttatcgttta gataccattc gatatcgacc gagctttgat acgatatgga
     3241 ccacgcttgc ctctgatgta aacatgagtg tgtataacac ggatgcggtt ggtaatacct
     3301 gggtcttatc gcaagctgat tgggatcaga tggtggaaga tgagatctat tatctctatt
     3361 tcaggatcaa tgataccctg ggcaatacgt tagagatcct gaatgacgat caggcaatca
     3421 cgattcgaaa ggataccttg ccaccactgg tcagcatcga tgttccctct gtggaagacg
     3481 aatggagcat gtctgataac tttaccgtct cagggcttgg cagtgatcat gatgggagcg
     3541 gtattaaaga agcctcattg tactatcgct tttctgagga taaatccaac tggagcagtt
     3601 ggactccatt tggtgatatg cttgattcct ccccgtttga atgggagttc gacgcgaccg
     3661 aaggtgacgg gtattatgag atgaaaatca tggccactga ttatgcgggt aacgaggtcg
     3721 aatccgaggt gttccccttg gctattgctt ccttcccgat gacccttgcg ctggtcctag
     3781 ttggtcttgt cacagtcctg ttgcttctca gtgtcattat ctaccttaag tggagaaaaa
     3841 aagagacagc ttaagggttc ttttctttta tcttttttac atcttcagca gtgtactccg
     3901 ggaagaacaa tccggtgtat ccacagctgt tgcatttcca ttggttcagg agtcctttag
     3961 cgtatgaatc tgctgagaga tctggttgta catcagtgct attacataca gggcagattc
     4021 gaacatattt cttctgcata gtatcactca tattattcta tatatatagc gggtcgtaag
     4081 ggactggcga atcttgtttt tttcgccggg tcgtacagat tgagatcatc tgcactatag
     4141 atctccacag ggcagtgcaa cacctcgctg agatatggtt ttgcatcgga gagatgagct
     4201 ttctcatcga ttgctatcag aaagcgtccg cggtccgtat cactgagcat ctttacctct
     4261 ggggcgatct ttgctacaaa ctgggagacc tgctgtccga gtggtttcat ccttgggtct
     4321 gccgtggttt ccttgatcag ctgaccaacg ttgaacgtct tttgagcaga ctgttccagt
     4381 gctttcctat agatgttttg tttccatgtt ggtgaggtgt agatacagat ttttttcggg
     4441 gtcatctttg tgactttcac gatttcgttt acatcctcaa tcaccctggt gaggaggtat
     4501 tctccgattt cgtctttttc ggagatgtcc tggggtttga actcagggta cgtttcattt
     4561 gagacaaact ggttgtatcc ttgtgctgtc cagagttcct cagtaagatg tggtgtgatc
     4621 ggcgtcatca ggatgatcca ggtgtggaca taatgcgtca gcagttgttt atctgttcct
     4681 cctcgtttca ggtaccattg aaggtctttt tggatctcaa aaaagatttc attggaggcg
     4741 acccgcagat cccaggtccc aaacgcatca aggattttct gtatatgccg ttgaagcatg
     4801 ctt
//
LOCUS       DATMXJ010000253         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_36602, whole genome shotgun sequence.
ACCESSION   DATMXJ010000253 DATMXJ010000000
VERSION     DATMXJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36602"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..3487
                     /locus_tag="VN377_06605"
     CDS             131..3487
                     /locus_tag="VN377_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWR28088.1"
                     /translation="MLEQLGRIIEKRPWLVVGVILLITIGFSLFIPSLTFKTNFEDFA
                     PDNEQVRANERIADYFGMSDQTIILYIQKEQTDSIISVQALRDQYELQKELTAMPEVE
                     GTISIFTFLDPVCQMEFNRTVDTCSDEQLKIAVNDLLNEPVSGNMTIFQTDDPNEPID
                     YQRSFLRSSGRAVDSADIKNCYILKDNKTLTFSFEVYSLSDFSSNLKPPLSKVSTMEW
                     YLDFKNSLLPSEYDMGYQIAARIEPTVPRWEIGNGLLGNIKQFIQNIRNHELTSYKKT
                     AYLWLRPPGQEMFFPLQLKTGNVTFNQETNRINVIVSRKELSGYGIALQVGSFELPTK
                     LTNFSAGVRYYRTPVLHRPGGRIVINTSYLFDRLQRLQSRPVLGSLISRMLQRFDINL
                     DDFSGLSENMTGTGFLPSTFSLSAIQTLWVQADRAPDSGVSTTIYPLIPELFSELRVN
                     ALSFISNEYSQTNTSHASLCIVQLNLQSGDAEELAKVNDKIIDKINDLNRQYTSISTQ
                     ATGEGIVTTQINDVAMDAMTIIGPAIFIIILCILFIAFRKPSYIILPVIVFAFSCVWL
                     FGTMALLGISFNIIAVALLPLNIGLGVEYSVNLLFNYRTEINKGRTPAEAIRLSIKEV
                     GIAIFLAWFTTFVAFLSFLSATLPPVRDFGIFLALGITYTFIITMTLLVALRYIIDRR
                     KPAVHLTSKEQTSSMTIIMSRVAHVLLRHEKKVFLATILICLVMGTAVTQLKSGFSMN
                     QFIPQDNPAIQLFTQIGEDFPFSSQDQEYILIEGNVATVQTLRGLQTTHEKMNDDLYV
                     ARRPDGSTKTESIFTLIQQAVANNHSLVELFNIDEETGLPRTDTDVQHAYDYLYESAE
                     YEMQVAGLLRRDGDEYTATILRVYVDLGSDSDDMTEQFEQLNAELTDDLSEYGDTTSI
                     ITGNLLITLSILKNMTESQILSTGICFVLAAIMLTLIYRNPLLGLIAMIPVTISIIWV
                     LGSMYFIGYTLNILTITVTSITIGVGIDYACYITERFRLVADQTGDVTKAVTETISRT
                     GSAVLIAALSSMFGFGVLAFAPIPPEQQFGIITAITLTYAFITSILVLPLVLARWAYW
                     RKKRKGYIISPGPPKKLDGIQTDSEYSGEQ"
     gene            3561..4532
                     /locus_tag="VN377_06610"
     CDS             3561..4532
                     /locus_tag="VN377_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWR28089.1"
                     /translation="MKKILVTGAVGQIGSELTTLLRKKFGNDNVLATGRKTKPSETLL
                     SSGPFEYIDICKRESIEKVVNDYDIDTIYNMAAILSAVGEEKPLLAWDVNINGLYNCL
                     EVAREHSMVRVFVPSSIAVFGPETPRDNTPQETILRPKTMYGVTKVAGELLGDYYVKR
                     FNLDVRGIRYPGIISSETLPGGGTTDYAVEIFYEAIKQKKYTCFLRADTTLPMMYMPD
                     CLKGTLDFMDADVTKLKHHCDFNFASMSFSAEELAHEITQHIPEFTCEYKPDFRQAIA
                     DSWPRSIDDSSSRKEWGWKPKYKLSDMTKDMLQKLGKRYEEGSLLVS"
     gene            4649..>5662
                     /locus_tag="VN377_06615"
     CDS             4649..>5662
                     /locus_tag="VN377_06615"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWR28090.1"
                     /translation="MRGNIDSIRGYLGADADYLLKHTCMKIKKESLHLPGPDFIDRTF
                     FDTDRSTPVLRNLQSLFSHGRLAGTGYLSILPVDQGIEHSAAASFTPNPLYFDPENIV
                     KLAIEGGCNAVASTLGGLGAVARKYAPKIPFIVKINHNELLTYPNKYDQIMFADVDQA
                     WEMGAAAVGCTIYFGSEESTRQIQEVSQAFKYAHELGMATILWCYLRNSAFKVDGKDY
                     HAAADLTGQANHLGVTIEADLIKQKLPDITGGYLALNRGGVKYGKMDEKAYTELSSDH
                     LIDLTRYQVAHCYMGRVGLINSGGASGKDDFGQAVRTAVINKRAGGTGLISGRKAFQK
                     PFED"
BASE COUNT         1530 a         1214 c         1284 g         1634 t
ORIGIN      
        1 tcattaattc taataacaat attaatatat ttaaaagtta atataatgaa acagttcatt
       61 tgagcatatc aaatacctag aatcgcagaa aatattgatt gttttatacg gaaaagagag
      121 agcacagagc atgttggaac aactgggaag aatcatagaa aaaagaccat ggctcgtggt
      181 cggcgtcatc ctcctaataa ccattggttt ctccctcttt attccctcac tgacttttaa
      241 aacaaatttt gaggatttcg ccccagacaa tgaacaagta cgggcgaacg aacgtatcgc
      301 tgattatttc ggtatgagcg atcaaacaat cattctttac atacaaaagg aacaaacaga
      361 cagcatcatc agtgtccaag cattacgaga tcaatacgaa cttcaaaaag aactcacagc
      421 gatgcccgaa gtcgaaggta cgatatcaat attcaccttt cttgaccctg tatgccagat
      481 ggaattcaat aggactgttg atacctgtag cgatgaacag ttgaagattg cagtaaatga
      541 tcttctcaac gaaccagtat ctggaaacat gaccatcttc caaaccgacg acccgaatga
      601 acctattgat tatcagcggt cattcctgcg ttctagcgga agggcagtgg acagtgccga
      661 tatcaaaaac tgctacattt taaaagataa taaaacactt accttctcct tcgaagtgta
      721 ttccctttca gatttttctt caaacctaaa accgccgtta tccaaagtca gtaccatgga
      781 atggtatctt gattttaaaa actcgctact cccctcggaa tatgatatgg gctatcagat
      841 tgctgcacgg attgagccaa ctgttccacg ctgggaaatc gggaacggac tcctgggaaa
      901 catcaaacaa tttatacaaa atatcagaaa tcatgaactg acctcttata aaaaaacagc
      961 atatctctgg cttcgacccc ctgggcaaga aatgtttttc cctcttcaac tcaagacagg
     1021 aaatgtgacc tttaatcagg agacaaatcg gattaatgtc attgtgtcac gaaaagagct
     1081 cagcggatat ggtattgccc ttcaggttgg ttcatttgag ctccctacaa aattaacgaa
     1141 tttctctgca ggggttcgct attatcggac cccggtgctc catcgacctg gtggacgcat
     1201 tgtcataaac acgagttatt tatttgaccg tctccagcgt ctgcaatcca ggccggtcct
     1261 gggttccctt atctcgcgga tgcttcagag attcgatatc aatctggatg acttctcagg
     1321 tctttcagag aatatgacag gtactgggtt tcttccatct acgttctcat tatcagccat
     1381 tcaaacactc tgggttcagg ctgatcgtgc accagatagt ggtgtttcaa cgacaatata
     1441 tcccttgata ccggaattgt tttcggagct gcgggtgaat gccttatcgt tcatttcgaa
     1501 tgagtacagt cagacgaata catctcatgc gagtctttgt atcgttcaac tcaatttgca
     1561 aagtggtgat gctgaggaac ttgcaaaagt caatgataag atcatcgata aaatcaatga
     1621 tctgaatagg caatacacca gcatctctac tcaagcaacc ggggaaggca ttgtaacgac
     1681 tcagataaat gatgttgcga tggatgcgat gacgattatc ggaccggcta tttttatcat
     1741 cattctttgt atcctgttta tcgcgtttcg aaaaccatcc tatatcattc tccctgttat
     1801 cgtgtttgct ttttcttgcg tgtggctgtt tggaacgatg gctctgcttg gaatctcatt
     1861 taacatcatc gcagtcgccc ttcttcctct gaatatcggt ttaggggtcg agtactcggt
     1921 caatcttctg tttaactatc ggacagagat aaacaaagga cgaacacccg ctgaagcgat
     1981 ccgtctttcc ataaaagaag tcggtattgc gattttttta gcatggttta ccacatttgt
     2041 cgcgtttctt tcgttcctca gcgcaacgct tccgcctgtt agagacttcg gtattttcct
     2101 tgctttaggt attacctata ccttcatcat cacgatgaca ctgcttgttg ctctccgtta
     2161 cattatcgat cggagaaagc cggctgttca tttgacctcc aaggaacaaa cctcttcgat
     2221 gaccatcatc atgagccggg ttgcacacgt actcctgcgt catgagaaga aagtgttcct
     2281 ggccactata ctcatctgtc tggtgatggg aactgcggtg acccaattga aatctggatt
     2341 tagcatgaat caatttatcc cacaggataa tcctgcgatt cagctcttta ctcagattgg
     2401 tgaggatttc cccttctcaa gtcaggatca agagtatata ctcatcgaag ggaacgtcgc
     2461 gactgtccaa acattacggg gtcttcagac cacgcatgaa aagatgaacg atgatctcta
     2521 tgtcgctcga agacctgatg ggagtacgaa aacagaaagc atttttaccc ttattcaaca
     2581 ggcagtggca aataaccatt cccttgtaga gttgttcaat atcgatgaag aaacaggatt
     2641 gccaagaacc gataccgatg ttcaacacgc gtatgattat ctctatgaaa gtgcggaata
     2701 tgagatgcaa gtcgctgggc tgcttcgcag agatggggac gaatataccg cgactatttt
     2761 gcgagtctat gtcgatcttg gttcggattc cgatgatatg actgaacagt ttgaacaatt
     2821 gaatgcagaa ttaactgatg atctttcaga gtatggtgat acgacatcca ttatcacagg
     2881 taatctgctc atcacgttgt cgatactgaa gaatatgaca gaaagccaga ttctgtctac
     2941 tgggatttgt ttcgtgcttg cggcgattat gcttactctc atttatcgca accctctgct
     3001 tggactgatt gcgatgattc ctgtcaccat ttctattatt tgggtcctgg gaagcatgta
     3061 ttttatcgga tatacgttaa acattttgac gatcaccgtc accagtatca ccattggggt
     3121 tggtattgac tatgcatgtt atatcacgga acgattccgt ttggttgccg atcaaacagg
     3181 ggatgtgaca aaagcggtca ctgagacgat ctcacggaca ggcagtgctg tccttatcgc
     3241 tgctctttcc tctatgtttg gttttggtgt ccttgcgttt gcccctattc cccctgaaca
     3301 gcagtttggt atcatcaccg ctattacact tacctacgca ttcattactt ctatcttggt
     3361 cttaccctta gttctggctc gatgggcgta ctggaggaaa aaacgaaaag gatatattat
     3421 tagtccagga cccccgaaaa aattagacgg tattcaaact gattctgagt actctgggga
     3481 acagtaaagt atctatagga aataatgatt ccagtgaaag aaaatacttt gggggttacg
     3541 cgattatggc acaaaaacgt atgaaaaaaa ttttggtgac cggcgccgtc gggcagatcg
     3601 gttcagagct tacaacgctg cttcggaaaa aatttggcaa cgacaacgtg cttgcaaccg
     3661 ggcgaaaaac aaaaccgagt gaaacacttc tcagctctgg tccctttgag tacatcgata
     3721 tatgtaaaag agaatccatt gagaaagtcg taaacgacta tgatatcgac accatctaca
     3781 acatggctgc gattctctct gctgttggcg aagaaaaacc tcttctggct tgggatgtca
     3841 atattaatgg attgtataat tgtttggagg tcgcacggga acatagcatg gttcgagtgt
     3901 ttgttcctag ctccattgcc gtctttggcc ctgagacacc tcgagataac acgcctcagg
     3961 aaacaatcct gcggccaaaa acaatgtacg gggtgacaaa ggtcgctggg gagctgcttg
     4021 gtgattatta cgtgaaaaga tttaatctcg atgtccgtgg gattcgatat cctggtatca
     4081 tcagtagcga gacactccct ggtggaggaa ccaccgatta tgcggtagaa atcttctatg
     4141 aagcaatcaa gcagaagaaa tacacctgtt tcttacgagc agacactaca ctaccaatga
     4201 tgtatatgcc tgattgtcta aagggaaccc ttgattttat ggatgctgat gtgaccaaat
     4261 tgaaacacca ctgtgatttc aattttgctt ccatgagctt ttccgctgaa gagcttgctc
     4321 atgagattac gcagcatatc cctgaattta cctgtgagta taaaccggat ttccggcaag
     4381 cgatcgctga ttcttggcct cgctcgattg atgatagttc atcgcggaag gaatggggtt
     4441 ggaaaccgaa gtataagctg tctgatatga cgaaggatat gttgcaaaaa ctcggtaaac
     4501 gatacgaaga gggtagcctc ctcgtttcgt aatcgttcat cctttttttt attttttctc
     4561 cgcagaaagt tttatgtgct atgcgatatt cccttgttta ttgtgaggtg aaccgctctt
     4621 gtcacaatct gaggaagttg gagtaaatat gagaggaaat attgattcta ttcgagggta
     4681 tttaggtgcg gatgctgact atctgcttaa acatacatgt atgaaaatta aaaaagaatc
     4741 attgcatctt cctggaccgg attttatcga tcgtactttt tttgatactg accggtcgac
     4801 ccctgtgctt cggaacttac agtccttgtt ttctcatggc aggttagcgg ggaccgggta
     4861 tctttcgatc ttgccggtcg accagggtat tgagcattct gctgctgctt cctttactcc
     4921 caacccgctg tattttgacc cagaaaatat cgtcaaactc gcgatcgagg gcgggtgtaa
     4981 cgcggttgct tctacccttg gtggcctggg agctgtagcc cggaagtatg ctcctaagat
     5041 tccgttcatt gttaagataa atcataatga gttgctcacc tatccgaata agtacgatca
     5101 gatcatgttc gctgatgttg atcaggcttg ggagatgggt gctgctgcgg ttggttgtac
     5161 catttatttt ggttctgaag aatcgacgcg tcaaatccag gaggtcagtc aggcgttcaa
     5221 atatgctcat gagctgggga tggcgacgat cctgtggtgt tatctgcgga attcagcgtt
     5281 taaggtggat ggcaaggatt accatgctgc tgcagatctg accggtcagg ccaatcacct
     5341 aggggtgact attgaagctg atcttatcaa gcagaagctc cctgacatca ctggcgggta
     5401 tctagcgttg aatcgtggtg gtgtgaaata tgggaaaatg gatgagaagg cgtatacgga
     5461 gctttcttca gatcatctga ttgatttgac tcggtaccag gtcgcccatt gttacatggg
     5521 gcgtgtcggg ttgatcaact ctggtggtgc ctctgggaag gatgattttg gtcaggcggt
     5581 gcggaccgcg gtcatcaaca aacgtgcggg tggtaccggt ttgatttctg ggcgaaaagc
     5641 gttccagaag ccgtttgagg at
//
LOCUS       DATMXJ010000254         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72521, whole genome shotgun sequence.
ACCESSION   DATMXJ010000254 DATMXJ010000000
VERSION     DATMXJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72521"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VN377_06620"
     CDS             complement(<1..563)
                     /locus_tag="VN377_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWR28091.1"
                     /translation="MILKSIHLKNFRKFKESYVEFPDGVTGVVGLNGVGKSTLFEAVA
                     WTLYGPVAARTSTEQIKREGAASSEPCRVELDFIFDDDAYHVVREMSGKNLSASGSAT
                     VNGKLAVNGAEVLSKFIQKKLGMDWKSFYTSIFAKQKELNTLSSMNPSERRQLILRML
                     GINAVDEIISQIRSDIKEKKNLIDNLAG"
     gene            complement(560..1696)
                     /locus_tag="VN377_06625"
     CDS             complement(560..1696)
                     /locus_tag="VN377_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HWR28092.1"
                     /translation="MRIVHLADTHLGYSAYRKVTEQGVNQREMDVYDAFTRCIDYAIK
                     TKPDLVLHAGDLFDSVRPSNRAITVAIQQILRLSKEKIPFVVISGNHETPKLKETGNI
                     FTIFEHLDQVYPIHKNHYEAIFLKRKDETVCVHAVPQCQNPDEFDINLKKISVDKNAD
                     YNLLLAHGAVKGIKEFKMNEFNELFIPIKNLSQDFDYIALGHYHIHTKLEENAFYAGS
                     TEHLSFTEADSQKGCLEIELGQKLRHRFIPLHTRTMIDVLPLDCSTLRIEQIVQKVKE
                     TIHGIEPKDKIIRLRLENIPAHIQRGIDYHQIRELGKTAVHFEVKTIPVKTDKTAAEE
                     GYKIKSLADEFGKFLTTQQYPEEKMLLNLGLQYIQKVEEHGKKP"
     gene            complement(1693..>2316)
                     /locus_tag="VN377_06630"
     CDS             complement(1693..>2316)
                     /locus_tag="VN377_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWR28093.1"
                     /translation="PETSLAKTIGCSIGKTGGVIVNEKTQTSLKNVYAVGDCTEYKDF
                     VTNEPLGVGLGSIVVRQGITAGTNAAGGTYSLPPGVLMTRTSEFFGIEIAAVGPVKHG
                     MKNLRLVSGKYKGFSKSDYFPGKDSITIKVGAEEETGRIVSAQAVGSNAAQRINTLAC
                     AVLSKMTIEEFRKLETTYAPPIAPTLDAVTLACDVVALKLGRKRREP"
BASE COUNT          553 a          543 c          499 g          721 t
ORIGIN      
        1 ccagcgagat tgtcgataag attttttttc tcctttatgt ctgatcgaat ttgactgatg
       61 atctcatcga ctgcgttgat cccaagcatc cgtaatatga gttgtcgtcg ttcactcgga
      121 ttcatcgatg acaaggtgtt aagctctttt tgtttggcaa aaatggacgt gtagaacgat
      181 ttccaatcca tcccaagttt cttctggatg aatttactca gcacctcagc accgttcacc
      241 gccagtttcc cattgacggt cgcgctacct gatgctgata ggttcttccc actcatctct
      301 cgcacaacat ggtacgcgtc gtcgtcgaag atgaagtcta gttcgacccg acacggctca
      361 gaggatgctg ccccttctcg tttgatctgt tctgtcgagg ttcgtgcggc aaccgggccg
      421 tacaatgtcc aagcaactgc ttcaaacagg gttgattttc caacaccgtt taatccaaca
      481 acaccggtga ctccatcagg gaattcaacg taactttctt tgaacttccg aaagtttttc
      541 agatggattg atttcagaat catggttttt tcccgtgttc ttcaaccttt tggatgtact
      601 gcaatccgag attcaggagc atcttttctt caggatactg ttgggtggtg aggaatttcc
      661 caaattcatc tgccagtgat tttattttgt acccttcttc tgctgctgtc ttatcggttt
      721 ttaccggtat tgtttttacc tcaaaatgca cagcggtttt cccaagctct cggatttgat
      781 gatagtcgat gcctcgttga atatgtgcag ggatgttctc cagacgaagc cggattatct
      841 tatcctttgg ttctatccca tggatggttt cttttacttt ctgtacgatt tgctcgatac
      901 gaagcgttga acaatcaaga ggaagaacgt caatcatggt cctagtgtgc agaggaatga
      961 accgatgtct tagtttttgc ccaagctcga tttcaagaca tcctttctga gaatctgcct
     1021 ctgtgaaact cagatgttct gttgatcctg cataaaacgc gttctcctct agtttggtgt
     1081 gtatatggta atgaccaagc gcgatatagt caaaatcctg cgaaaggttt tttatcggga
     1141 tgaataactc gttgaattca ttcattttga attccttgat accttttacc gcaccatgcg
     1201 caagcaatag attgtaatcc gcgttcttat ccactgaaat ttttttcaaa ttgatgtcaa
     1261 attcatcagg attttgacat tgcgggactg catggacgca aaccgtttca tctttccgtt
     1321 tcaaaaagat tgcttcatag tgatttttat ggataggata cacctggtca agatgctcaa
     1381 agatagtgaa gatattcccg gtctccttga gttttggtgt ctcatgattc ccagaaatca
     1441 caacgaaggg aatcttttct tttgataagc ggagaatctg ttggattgca acagtgatcg
     1501 ctcgatttga cggacgaaca gaatcaaaaa gatcaccagc gtgaaggaca agatcaggtt
     1561 ttgtttttat tgcataatca atgcatcggg tgaacgcatc atacacatcc atctctcgtt
     1621 ggttcacccc ttgttctgtt actttcctgt atgcagaata accaagatgc gtatcagcaa
     1681 gatgtacaat cctcatggct cccttctttt acgtccgagt tttaacgcga caacatcaca
     1741 ggctaaggtc acagcatcca gtgtcggtgc aatcggtggc gcgtacgttg tttcaagctt
     1801 tctgaactct tcgatggtca tcttactcag aaccgcgcat gccagggtgt tgatccgttg
     1861 tgctgcgttg ctaccaaccg cctgggcgga gacgatacga ccggtctctt cttcggcacc
     1921 aaccttgatg gtgatggaat ctttacccgg gaaataatca gacttggaga aacctttgta
     1981 tttaccgctc accaaccgca ggtttttcat cccgtgtttg acaggaccaa ctgcggcgat
     2041 ctcgatacca aaaaactctg aggttctcgt cattaatacc cctggaggta atgagtacgt
     2101 tccacctgca gcgttcgtac ccgcggtgat cccttgtcgg acgacaatac tcccaaggcc
     2161 gacaccgaga ggttcattgg tgacaaaatc cttatattcc gtgcaatcgc cgactgcgta
     2221 gacgtttttc agagaggtct gtgttttttc gtttacgatg accccaccag ttttaccaat
     2281 cgaacatccg atggtttttg caagagacgt ttccgg
//
LOCUS       DATMXJ010000255         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72543, whole genome shotgun sequence.
ACCESSION   DATMXJ010000255 DATMXJ010000000
VERSION     DATMXJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72543"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(511..966)
                     /locus_tag="VN377_06635"
     CDS             complement(511..966)
                     /locus_tag="VN377_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28094.1"
                     /translation="MKNQKKILYFLKNESAVLGLPMRLTVSLIIGSIALIAILSTILN
                     PCLFPHRMLVTISPQVTTVQGSDPENVSFTVFVNDSDGFPLSGASVIIKGLGGAGSGV
                     SENDGKALVNLEIQLENGTYEGYLDISVRAPCHVPFEYQAIVKVVKENR"
     gene            1047..1553
                     /locus_tag="VN377_06640"
     CDS             1047..1553
                     /locus_tag="VN377_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28095.1"
                     /translation="MIFRRAHVLSVDRDAVVGQPISLLVILLVAGLITTFLCLSIPNL
                     MKESQIQKVEGEIDRILTEATTMFEYADNGSLRTVSVEFPLSMRFVVFGQVPRDGTNE
                     PVNLTLDENTSNNYYYVMDDGTVRTFHSNARFSNHNITQMALFHTGTYDITLELCQKE
                     GKTYLTMQ"
     gene            1570..2142
                     /locus_tag="VN377_06645"
     CDS             1570..2142
                     /locus_tag="VN377_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28096.1"
                     /translation="MGLTLTQAGLVLVTGILLTVVLSMVFSNDWQRTAELQSQASSFS
                     NLLGDIDNSFFEQSTRFQFSHTTYPYSVTISTEYILIAAKGSWGSDLIVTKKLFFPPW
                     PRLPQQNWTTGEDLHTYLNRTCGHRGTQNDSIPSENFTQLCQEQNNTVSFFALHPLEI
                     LLKEPVFIEKVTIFYDQTKKHDFLLIYQIV"
     gene            2314..3051
                     /gene="pcn"
                     /locus_tag="VN377_06650"
     CDS             2314..3051
                     /gene="pcn"
                     /locus_tag="VN377_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HWR28097.1"
                     /translation="MFNAKIKSDIIKGIIDVTSPLVNEAKFNISSKGISIRAVDPAHV
                     AMVDLELKSTAFEEYKADDMELGIDLDKLNGIMKLANSGDSISMEYDEESNRLIVKIG
                     NLVRRMGLIDTAGMPDSKVPHLDLPAKAVVKASELSKGVRASEAVSDHLALSVDKDSF
                     ELFAEGDTDTVNLKLPKNLLVELHSPGKFKSLFSIDYFSNMIKPVKSDDPITINIGND
                     NPIRVDFDFAEKQGHVTYLLAPRIESE"
     gene            3093..3422
                     /locus_tag="VN377_06655"
     CDS             3093..3422
                     /locus_tag="VN377_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HWR28098.1"
                     /translation="MKGTRRHRYIDFHVDSKEQNFSFTNSELLQALRQQTFEIFSKNL
                     KDMRLWVVQFDGAQGILKCHYTEKEHVIQLLLSIKKIGTKPVTVTTRSTSGTIRGLAH
                     KKQTHRQ"
     gene            complement(3473..>4051)
                     /locus_tag="VN377_06660"
     CDS             complement(3473..>4051)
                     /locus_tag="VN377_06660"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557437.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWR28099.1"
                     /translation="FLPIPRITKKQNTFCFDYNYPDSIGKIKATYGNISVILKAYIYI
                     LLMGGNGLKEASEIAVLNANYMKKKLVDSTLYQLPYQELRKHEFVISCEQLRSTKGIR
                     ALDVAKRLLDYGLHPPTIYFPHIVKEALMIEPTESESKKTLDEYIDILLRIAQEDPDL
                     VKDAPHNTAVQRIDEIGAVKNPILTWKDSGRL"
BASE COUNT         1177 a          875 c          796 g         1203 t
ORIGIN      
        1 cgcctgtggt tttatttcct gtagagttgt tacaaccggg tatgattctt ttttgaattc
       61 ttcagggttc ttgactttcg tttccactga tttaacttcg tgaaataatt ttagtatttc
      121 ttcgtgagtt tttaccttcg aagaatcttc attttggttg tccacggtat tgttcgcccc
      181 tcactgttac ctatctgaaa tccttataag ataacagatt aatataaaaa aacctatccc
      241 ttagtttcaa ttctctcgag ttgttatctg agaggatttt agattacctg tattttctca
      301 tatcgcagat tcgattcatc acaacatgaa cagtattctt ctactattat gaccgaaccg
      361 attttgcgta cgggaatttg tttcatttct tttttacata ctctacagta actcaaaaac
      421 aatgccccaa tatgtattag taccaagtat tatataaata tttccataaa tatcgttatt
      481 ttctcctgga aattcatccg gatgtccttt ttatcgattt tcttttacga cctttactat
      541 cgcctgatac tcaaaaggga catgacaggg tgccctgaca gaaatatcaa gatatccttc
      601 gtaagtgcca ttttctaatt gaatctccag attcactaat gctttaccat cattttcaga
      661 aacaccagag ccagcaccgc ctaatccttt gatgattacg gaagcaccgc ttaggggaaa
      721 tccatcggaa tcgtttacga acacagtaaa actcacattt tcagggtcgc tgccttgtac
      781 ggttgtcact tgcggagata tcgtaaccaa catccgatgg ggaaacaaac aagggttgag
      841 aatcgtcgat agaatcgcga ttagggcgat ggatcctatg atcaacgaga ccgtcaggcg
      901 catgggaagc ccaagcactg cggattcatt cttcagaaaa tagagtatct ttttttgatt
      961 cttcatgatt caacctccca gacgaaacgc aatcgataaa tatataatga aatatttaat
     1021 tctttgtaat aaatctggga ggttttatga tctttagaag agctcatgtt ctgtctgtcg
     1081 atagagacgc tgtcgttggt caaccaatca gtcttcttgt gattctcctc gtcgctggtc
     1141 ttatcaccac atttttgtgt ctctccatcc ccaatcttat gaaagagtcg cagatacaga
     1201 aagtcgaagg cgaaatcgat aggattctga cggaagcgac aactatgttt gaatatgccg
     1261 ataacggatc tcttagaacc gtatctgtcg agttcccact atcgatgcgt tttgtcgttt
     1321 ttgggcaagt cccaagagat ggcaccaacg aaccagtaaa tctcactctc gatgaaaata
     1381 caagcaacaa ctactattat gtgatggacg acggaacggt gagaacgttc cattccaatg
     1441 ctcggttctc taatcacaac atcacgcaaa tggcactctt tcatacagga acctatgaca
     1501 tcactctgga gttatgccaa aaggagggga agacctatct tacgatgcag taacgataca
     1561 aaaggcatca tggggttaac actgacccag gcaggtcttg tcctagtgac tgggatcctc
     1621 cttactgtcg tcttatccat ggttttctcc aatgattggc aacggaccgc tgaacttcaa
     1681 tcacaagcga gtagtttttc aaatcttctc ggagatatcg ataacagctt cttcgagcag
     1741 tcaaccagat tccaattctc tcacaccacc tatccatatt ctgtgacaat ctcaacagag
     1801 tacattctca ttgctgcaaa aggatcctgg ggcagtgacc ttatcgtgac aaaaaaatta
     1861 ttcttccctc cttggcctcg tctccctcaa cagaattgga ccaccgggga ggacctccat
     1921 acctatctga atagaacctg cgggcatcga ggaacccaaa atgattccat cccttctgag
     1981 aatttcaccc aactctgtca agaacaaaac aacacggtat ctttttttgc gttacatccc
     2041 ttagaaatcc tcctcaaaga accggtcttt attgagaagg tcaccatttt ttatgatcaa
     2101 accaaaaaac atgattttct cctcatctac cagatagtat aagcataaat gcctctgtac
     2161 catgtgtcgc ttttcgcata aagaacctta gaacggtggt tcccctccca aagacagttt
     2221 gaatggagcg gagaaaaata ccgtcatggt tatataggca atgtcccatt ctttccctaa
     2281 gaagagaact aagtatcggg gaggcagcgt gccatgttta atgccaagat aaaatcagat
     2341 attataaaag ggatcatcga tgtaacatca ccactggtga acgaagcgaa attcaatatt
     2401 tcctcaaaag gaatctcgat acgggctgtt gatccagcgc atgtcgcaat ggtggattta
     2461 gaactcaaaa gcacagcgtt cgaggaatat aaagcagatg acatggagct tggtatcgac
     2521 cttgataaac tcaatggcat tatgaaacta gccaattccg gcgattcgat ttctatggag
     2581 tatgatgagg aatcaaatcg cttaatcgta aaaatcggca acctcgtacg acgaatgggt
     2641 ctgattgata ctgcagggat gcctgattcg aaagtacctc atttagatct cccggcaaaa
     2701 gctgtcgtaa aagcatcaga gttgagcaaa ggagtacgag cctcagaagc agtctctgat
     2761 catcttgcct taagcgttga taaggactcc tttgagttgt ttgcagaagg agatactgat
     2821 accgtcaatc tcaaactccc aaagaacttg ctggtggagc tccactcgcc agggaaattc
     2881 aaaagtcttt tttcaatcga ctacttcagc aatatgatta aacctgtgaa aagcgatgat
     2941 cctataacca tcaacattgg caatgacaac cccattcgcg tcgactttga tttcgcagag
     3001 aaacaagggc atgtgacata tctgctagcg cctcgcatag aatcagaata atacctccgt
     3061 tttttcacat cacttgagag gtgctctgta tcgtgaaagg taccagacga catcggtaca
     3121 tcgattttca tgttgattcg aaagaacaga attttagttt taccaattca gaactcctcc
     3181 aagcacttcg acaacaaacc tttgagattt tttcaaagaa tttaaaagac atgagactgt
     3241 gggtggttca attcgatggg gcacagggta ttctcaaatg tcactataca gaaaaggaac
     3301 atgtgataca gcttttatta tcaataaaaa aaattggaac aaagcctgtc acggtcacaa
     3361 cccgttcgac atctgggacg attcgtggac ttgcacataa aaaacagaca cacagacagt
     3421 aatatagaaa ggaacctttt ttttcgattc tcacaatcta acgatttctt tcttagagtc
     3481 ttccactatc tttccaggtt aggatagggt tttttactgc cccgatttca tcgatgcgtt
     3541 gcaccgcagt gttatgagga gcgtccttca caagatccgg gtcttcctgt gcgattcgta
     3601 gtaggatatc gatatactca tctagtgttt ttttcgattc tgattcggtt ggttcaatca
     3661 ttaatgcttc tttcacgata tgcggaaaat atattgttgg tggatgaagt ccatagtcca
     3721 agagacgttt tgcgacatcc aacgcacgga tgccctttgt cgaacgcaac tgttcgcaac
     3781 tgatgacaaa ctcgtgtttt cgaagttctt ggtatggaag ttggtacagg gtggaatcga
     3841 caagtttttt cttcatatag tttgcgttta atacggcgat ttcagaagct tctttcaacc
     3901 cgttacctcc cattaaaagg atatagatat atgctttgag aatcacggag atatttccat
     3961 aggtcgcttt tatttttcca atggaatcag gatagttata atcgaagcag aaagtatttt
     4021 gctttttcgt aattcgtggg atggggagga a
//
LOCUS       DATMXJ010000256         2064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_9661, whole genome shotgun sequence.
ACCESSION   DATMXJ010000256 DATMXJ010000000
VERSION     DATMXJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9661"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..>2064
                     /locus_tag="VN377_06665"
     CDS             289..>2064
                     /locus_tag="VN377_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR28100.1"
                     /translation="MSVYLKQVEMENFKSFGHKLTVPFYPGFTAITGPNGSGKSNIVD
                     AILFVLGPKSSKVMRAGRLTDLIFNGGKNRKNPSKYCKVSLVFDNSQRKMQIAADNVV
                     LTRMIKRAPLKDNPDNYYSYFYINNRAATYTEFIDLLTHARISGEGYNIIKQGDVTSL
                     VEMGSVDRRKIIDGIAGISTFDEDIEKAEKERGDVDKNLERIDIILNEITSQIRQLKK
                     DRDAAYQYKELKDKLYETKAMIAYKKKQEIESQILEVNKQIESYEQEQKKFEGQKETL
                     KTQYTELQQKLDEIEKKIGVAGGDEAAGIKEKIDTLRSETIKMDERINYSNDEVTEKK
                     KEKEELETSLDEITKELDEYTKQKEELTTKIQDVETALKTKEDELGKLREEIAQSDDT
                     SMDITRELVKMREEYNNTQNEKHDFELTRDRLKEKQDAFELQIAELQETKSTYEFELK
                     DIDWQVGELQKGKQEGSKKLKESEKQLFEKKKRESEITEQLADLEKAILKLQREQSKL
                     QAERDAVQTIGAKYNSAVESILAARNSGELKGVRGTIAELAQVDERYKLPLEIAAGPR
                     MQAVVVENDAAAAEAIGYLQKKKLGR"
BASE COUNT          753 a          397 c          458 g          456 t
ORIGIN      
        1 tctctatcct gtgctatact aggaattaca ctaattccca tcaataaaag aatgaatccg
       61 acagccaatc cttttgtatt caatgatttt tctcctttgt aaaattttat aacactgttc
      121 cgcttaaaaa acatttcttc aagagagtag taccataaat gccattatat aaaaatgagg
      181 tgaaatatac tgttatctcc aaaattttat atacagtttt ctgtttctcc ttattgggat
      241 gccggatatc gataacgtag gagcggatgt gctctctagt ggaaagggat gagtgtgtat
      301 cttaaacaag ttgaaatgga aaattttaag tcctttggac ataaattaac cgtaccgttt
      361 tacccaggtt ttactgcgat tactggaccg aatggatctg ggaaaagcaa tatcgtcgac
      421 gcgatactct ttgtacttgg cccaaaaagc tccaaggtca tgcgtgcagg tagactcacc
      481 gatcttatct tcaatggagg gaaaaatcgg aagaatccct caaaatattg caaggtctcc
      541 ttggtctttg acaattcaca acggaaaatg cagatcgccg ctgacaatgt cgtccttacc
      601 cggatgatca aacgagctcc cttgaaagac aacccagata attattacag ttatttttac
      661 atcaacaatc gtgcagcgac atacactgaa tttatcgacc ttctgaccca tgcgcgcatc
      721 tccggggaag gctataacat catcaaacag ggtgacgtca ccagtcttgt cgaaatggga
      781 tctgtcgacc gtaggaaaat cattgatggc atcgctggca tcagcacctt tgatgaggat
      841 atcgaaaagg cggaaaaaga acgaggggat gtcgataaga acctggaacg tatcgacatt
      901 attttaaatg agattaccag tcagattcga cagttaaaga aagatcggga tgccgcctac
      961 caatacaaag agctcaagga caaactgtac gaaacaaaag ccatgatcgc gtataagaaa
     1021 aaacaagaaa tcgaatcaca aattcttgaa gtaaataaac agattgagtc ctatgaacag
     1081 gagcagaaga aatttgaagg acaaaaagaa acattaaaaa cacagtacac agaactgcag
     1141 cagaaactcg acgagattga gaagaaaatc ggcgtagcag gaggagatga agccgccggt
     1201 attaaagaaa agattgacac gttacgctct gagaccatca aaatggacga acgcatcaac
     1261 tactcaaacg atgaggtgac tgagaagaaa aaagaaaaag aagaactgga aacatcactt
     1321 gatgaaatca caaaggaact cgacgaatac acaaaacaaa aagaggagct gactacaaaa
     1381 atccaggatg ttgagacagc actcaaaaca aaagaggatg aactaggaaa attacgagaa
     1441 gaaatcgcac aatcagatga tacatccatg gatatcaccc gagagcttgt caagatgcgt
     1501 gaggaataca ataatacaca gaacgagaaa catgactttg agctcacacg ggatcgattg
     1561 aaagaaaaac aagatgcctt tgagttacag atagctgaat tgcaagagac aaaatccact
     1621 tatgagtttg agctgaagga tatcgactgg caggttggtg aactgcagaa aggaaaacag
     1681 gagggcagta aaaaattaaa ggaatcagaa aaacagctct ttgagaagaa aaaacgggaa
     1741 tcagagataa cagaacagct tgcagatctt gaaaaggcga ttctaaaact ccagcgggaa
     1801 caatcaaaac tacaggcgga acgtgatgcg gtacaaacca tcggagcgaa gtataattcc
     1861 gcggtggaat ccattcttgc tgcacgcaac tctggggaac tcaaaggtgt tcgaggcacg
     1921 attgctgagc tagcgcaggt tgatgagaga tacaaattac cattggagat cgctgcagga
     1981 ccacggatgc aggctgtcgt cgtcgaaaac gatgcagcag ccgctgaagc aatcggatac
     2041 ttgcaaaaaa agaaattagg tcga
//
LOCUS       DATMXJ010000257         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30202, whole genome shotgun sequence.
ACCESSION   DATMXJ010000257 DATMXJ010000000
VERSION     DATMXJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30202"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VN377_06670"
     CDS             <1..1151
                     /locus_tag="VN377_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28101.1"
                     /translation="IIDMDGAFLKFANVYLIWEEENDEFNETEGNNLAGNGLNGEYTF
                     WIRKADQGGPLPKKITIAAQAYPGSSFWGYGKVISVPSIENIDFNIAYINDIYAELPW
                     GGSVMFTNASFGLIINTGPDTITYDVLKAAKITVTSDVEGASLDIGFNIYENSFPTIK
                     PKQAWGSIDDQNHFLVDFLKDGEELLNMTPHSTFYFKVSRQNFTGTAHFHITIQIGKR
                     ITFLETNVTYIDTSEHHVELIRGERVKSYRHYENTAAATFGPIAPILNFSSVHLINGD
                     PAQVQKIQRILKSRILQYIIPIIQFNITQLSFSVMYPREIPQKPFFQRYLYMTGIQQN
                     NITLINEPHQIIVSGMNGFFIMVKGAFFQINPPRFAFVGNCSDVTILT"
     gene            1165..2100
                     /locus_tag="VN377_06675"
     CDS             1165..2100
                     /locus_tag="VN377_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28102.1"
                     /translation="MNTSIDKKTFVVLSFFLCIGSIIAPISSTYTVRASTDNTPVKIT
                     TRVSDPTAETTVTVQLTKQQTQLVQEIFDDLKNRLSTADSIDATQRIFNDTIIELDRY
                     NLLPEGMSIEQAQHLVNRATNHRKIITPLQKLAQNLQAKTTGGTIQNSFCSMAGNTSN
                     THFTKLAKRTALRLYYIIDFNTGNAPLVKMATALFVVFNEIGKINQMILRQNGYHLGV
                     CIYFGNYHYAPYPNWLSPAQGWLSTNGASGKQNITGSFWGQTMTGGWQPQVDWHMNYT
                     WRGCLGFTGLIYYTDIDTAYYLGSALAVNVGPNRP"
     gene            2161..3036
                     /locus_tag="VN377_06680"
     CDS             2161..3036
                     /locus_tag="VN377_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWR28103.1"
                     /translation="MDNQLSPRGWQLHHHEQLFIKANSGAPTIRLKFYHYPSYRQTVI
                     INQPPADCKLKNKKDNMFFLFQQKVRTKATISLERIISIYPTPSIVSLHTSWGRISDV
                     PRALQRKYTQSFPYWPVDSPAIKDISQETWFSTDDLLTWVHAAYNYITMKIKYPEKQD
                     RRLGADQVLRTGSGDCDEFTDLFITLARIRGLPCRRLTGFFIHHANNEVEPHAWAEML
                     SPIHGWVPIDIALRNIGNHTINYVIGKIEEFNPSLLDYQILRQTTAVNYHWERPAPTV
                     TPLFEKKEKIQGNHP"
     gene            complement(3058..5112)
                     /locus_tag="VN377_06685"
     CDS             complement(3058..5112)
                     /locus_tag="VN377_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HWR28104.1"
                     /translation="MRNIISYCICISVLLSGLGIVGISGENLRNNHDVLLSPDSSPDL
                     SAVLYTATQSSLSEQTGIYPVMDTYPFDAGKGDYPLGDLIWQYQIGGGSDNSPKAIAA
                     LDDINADGFDDIVICSEDNTIRCFSGYANGSGEVLWTHEIYLGNIYQQHGLAITEDIN
                     GDGYNDCVVGATGGARLIRCISGFDGQTIWTHDTHEYGDGGWVYQVDCGYDYNGDGIR
                     DVVAATGDDFSETGPKRVYCLNGLTGVSLWERPMGGPGFAAMGVEDFTGDGVPDVLAG
                     CSNDAESTGYGKGINGATGALMWTYPAAGSSVWGVEQLDDVTGDGISDVIIGDFSGHI
                     YGLNAATGTQVYTLSISSVIITRLERLGDVNGNGYSDIIPAHSTTTTTQVIDGYTGGV
                     IWSHTVADQPWNVARIADITGDTIDDVLVGTLYNSNYVYFLNGVNGSELKKISYGQAV
                     DAIASIPDVIGDFSMEMVAGGREGKVNCLSGGENGTQQQVTITAEFTAAPLSGTVPLL
                     VQFTDLSTAENTTITSWEWDFDDDGIIDSNNPNPTWTYNETGTFTVTLTVSDGERSNT
                     EKKVDYITVLPAGTTIVEIGNITGGLFKVSAEIVNKATLNLTSLNWNITLAGGIIFVG
                     NYHSGTIASLPAGDTTVIADTGVIGFGRIGITVTVTVPDGEPITKTANGFLLFFVVIG
                     VK"
BASE COUNT         1622 a         1405 c         1081 g         1291 t
ORIGIN      
        1 caataataga tatggatggg gcgtttctga aattcgcaaa cgtttacctc atttgggaag
       61 aagaaaatga cgagtttaac gaaactgaag ggaataacct ggcaggcaat ggattgaacg
      121 gcgagtacac cttctggata cgaaaggctg atcagggagg ccctcttcca aaaaaaatta
      181 ccatcgcagc gcaagcctat ccgggatcat ctttctgggg atatggaaaa gtcatatcgg
      241 tcccatccat cgaaaatatc gattttaaca tcgcgtacat caacgatatc tatgcagaac
      301 tcccatgggg cggtagtgta atgttcacta acgcaagttt tggcctcatc atcaatacag
      361 gacccgatac gattacctat gatgttttaa aggctgcaaa aatcacggtc acatcagatg
      421 tcgagggagc aagccttgat atcggattta atatttatga gaactcattt ccaaccatta
      481 aacccaaaca ggcatggggt tcgattgatg accagaatca ttttctcgtt gattttttaa
      541 aagacggaga agagctgctg aacatgacac cccattcgac gttctatttc aaggtcagca
      601 gacaaaattt tacaggaact gctcactttc atatcacgat acaaatcggg aagagaataa
      661 cgttcctcga aacaaatgtc acgtatatcg atacatcaga acatcatgta gaactgatac
      721 gtggggaacg agtcaaaagc tatcggcatt atgaaaacac agcggctgca acattcggtc
      781 cgatcgcacc gatcctgaat ttttcttcgg ttcatctcat taatggggat cctgcccagg
      841 tacagaaaat acaacgcatc ctgaaatcaa ggattctcca atacatcata cccatcatcc
      901 aattcaatat cacacaatta agcttctcag tcatgtatcc gcgggagata ccacagaaac
      961 cttttttcca gaggtatttg tacatgaccg ggatacaaca aaacaatatc acgcttatca
     1021 atgaaccgca tcaaatcatc gtaagcggga tgaacggatt cttcatcatg gtaaaagggg
     1081 cgtttttcca gataaatcca cctcgtttcg ccttcgtagg gaattgttca gatgtaacga
     1141 tattgacata ggatttgatg aaaaatgaac acctcgattg acaaaaaaac gttcgtagta
     1201 ctcagtttct tcttatgcat tggttccatc atagcaccga taagtagtac gtatactgtc
     1261 agagcatcaa cagacaatac tcctgtgaaa atcacaactc gggtctccga ccctacagca
     1321 gaaacgacag taacagtcca attgacgaaa caacaaaccc aattagttca agaaatcttt
     1381 gatgatctca agaatcgctt atcaaccgca gattctattg atgccactca gaggatattc
     1441 aatgacacca tcatcgaact ggacagatac aatctcttac cagaagggat gagcattgaa
     1501 caggcgcaac acttggtcaa tcgtgcaaca aaccatcgaa aaataatcac acctcttcag
     1561 aaactcgctc agaacctcca agcaaagaca acaggaggaa ccatacagaa ctcgttctgc
     1621 tccatggcag gcaacaccag caacacacat tttacaaaac tcgcgaaacg aacagcacta
     1681 cgactgtact acatcatcga ttttaacaca ggaaatgctc ccttagtcaa gatggctacc
     1741 gccctgtttg tcgtcttcaa tgaaatcggg aagatcaatc aaatgatctt gcgacagaac
     1801 gggtatcatc tgggcgtctg catctacttt gggaactatc attatgcccc ataccctaac
     1861 tggttatccc ccgcacaggg atggctctca acaaatggag caagcggaaa acaaaacatc
     1921 acaggctcgt tctgggggca gacaatgaca ggcggctggc agccacaggt cgactggcac
     1981 atgaactaca cctggagagg atgcctcgga ttcacaggat tgatctacta cactgatata
     2041 gataccgcct actatcttgg ctctgccctg gcagtcaacg tcggaccaaa tcgaccttga
     2101 tcatctgaac atactcccaa gaaacctatt taaagacgtg tttcttcaaa gcaaatacct
     2161 atggacaatc aactttcacc tcgtggatgg caactgcacc atcatgaaca actgttcatc
     2221 aaagcgaaca gtggcgcacc caccatccgc ctcaagttct accattatcc ctcctaccgg
     2281 caaaccgtga tcatcaatca acccccggca gattgcaagc tgaaaaacaa aaaggacaac
     2341 atgttcttcc tcttccaaca aaaggttcgg acgaaggcaa cgatctccct tgaacggatc
     2401 atctccattt atccaactcc ctcgatcgtt tcactgcaca cctcctgggg gcggatctcg
     2461 gacgtccctc gagccctcca acgaaaatac acacaaagct tcccctactg gccggtcgac
     2521 tccccagcca tcaaggacat ctcgcaagaa acttggtttt ccacagatga tcttctcacc
     2581 tgggtgcacg cagcatacaa ttacatcacc atgaagataa aataccctga gaaacaggac
     2641 cgacgacttg gcgctgacca agtgctccgc actggaagcg gtgattgtga tgagttcacc
     2701 gacctgttca tcactctcgc caggatacgt ggtcttcctt gtcgacgatt aaccggcttt
     2761 ttcatccatc atgcgaacaa cgaagttgaa ccacacgcat gggcagaaat gctctcacca
     2821 atccatggat gggttccgat cgacatcgcg ttacggaaca tcgggaatca taccatcaac
     2881 tacgtgattg ggaaaatcga ggaattcaac ccatctcttc tcgactacca gattctaaga
     2941 caaaccactg cggtgaacta tcactgggaa cgaccagcac ccacggtgac acctttattt
     3001 gaaaagaaag aaaaaattca aggaaatcat ccttgaatca agggtgatgg aatactctta
     3061 ttttacgccg atgacaacga aaaagagcag gaacccgttt gctgttttcg tgattggctc
     3121 accatcaggt acagtgaccg tgacggtaat accaatccgt ccaaacccga tcacacctgt
     3181 atctgcaatg acagttgtat cacccgccgg tagagaagca atcgtcccag aatggtagtt
     3241 tccaacgaaa attatcccac cagcaagggt aatattccaa ttcaacgaag tgaggtttag
     3301 tgttgctttg ttaacgattt ctgcgcttac cttaaacaaa cccccggtga tgtttccgat
     3361 ttccacgatc gtggtccctg cgggaagaac tgtgatgtaa tcgacttttt tctccgtgtt
     3421 agaacgctct ccatcagata ccgttaatgt caccgtgaac gtcccggtct cattataggt
     3481 ccatgtgggg ttaggattgt ttgaatcaat aatcccatca tcatcaaaat cccattccca
     3541 ggatgtaatc gtggtgtttt cagcggtaga cagatccgtg aactgcacga gtaacggaac
     3601 agttccggaa aggggagctg cggtgaactc tgcggtgatg gtcacctgtt gttgagtccc
     3661 attctcacca ccagaaagac aattgacctt cccttctctc ccaccagcaa ccatctccat
     3721 ggaaaaatca ccgatgacat cagggatgct cgcgatcgca tcaacagcct gaccatacga
     3781 gattttcttc aactcagatc cattcactcc gttgaggaaa tacacataat tggaattata
     3841 caaggtccca acaagcacat cgtcgatggt atccccggtg atgtctgcga tccgtgcgac
     3901 gttccatggt tgatccgcta ccgtatgaga ccagatcacc ccaccagtat acccgtcaat
     3961 cacctgggtc gttgtcgtgg tgctatgagc agggatgata tcggaatacc cgttcccgtt
     4021 cacatcaccg agtctctcta aacgggtgat gatcacagag ctgatggaaa gggtatacac
     4081 ttgtgtcccc gtcgcagcat tcagaccgta gatatgacca gagaaatcac cgataatgac
     4141 atccgatatc ccatcaccgg taacatcgtc gagctgttca acaccccaga ccgaggaacc
     4201 agcggctgga tacgtccaca tcagagcacc tgttgctcca ttgatccctt ttccatatcc
     4261 ggtggattct gcatcattgg aacacccagc aagcacatca ggcactccat cgccagtgaa
     4321 gtcttcaacg cccatggctg caaaccctgg tccacccatg ggtcgctccc ataaagatac
     4381 cccggtcagc ccgttcagac aatacacccg cttcggaccg gtctcagaaa aatcatcccc
     4441 ggttgctgcg acgacatcac ggatcccgtc gccgttgtaa tcataaccac aatccacttg
     4501 gtagacccat ccaccgtcgc cgtactcgtg ggtatcatgc gtccagatcg tctgaccatc
     4561 gaaaccagag atacagcgaa tgagccgagc accaccagtc gcgccgacca cacaatcgtt
     4621 gtacccgtct ccattgatat cctctgttat cgccaacccg tgctgctgat agatattccc
     4681 caaatagatt tcatgagtcc aaagaacctc gcctgaacca ttcgcatatc cactgaaaca
     4741 gcggatcgta ttatcctcag agcagatgac gatatcgtca aacccatcag cattgatatc
     4801 atcaagagca gcaatcgctt tcggactatt atcagaccca ccaccgatct ggtactgcca
     4861 gataagatca ccaagaggat agtcgccctt gcccgcatca aaaggatacg tatccatcac
     4921 tggatagata cctgtctgct ccgacaaaga agactgagtt gcggtgtaca aaacagcaga
     4981 gagatcagga gaagaatccg gtgacaacag cacatcatga ttattcctca gattctcacc
     5041 agagatcccg acgattccaa gtccactaag aagtacactt atacaaatac aatacgatat
     5101 gatgtttctc atattatttt cccctaattt tatataaaag taaattttgt atataaatgt
     5161 atgcgaactt ctttttcgac gttttaaacg aaaaccccat gaaaaacagt attttgtcat
     5221 gataatttaa atactatagt tagtataata tgtcgggggt aaaacaaatt gaagaagagt
     5281 aaaatcattt gccttgttgt atgtatgctg atgatcgtga ctgtcttccc tgtcgttggt
     5341 cttccaaccc aaacagggga aacgaagaat atatctgtca cgcaaccacg aaatcaacc
//
LOCUS       DATMXJ010000258         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30239, whole genome shotgun sequence.
ACCESSION   DATMXJ010000258 DATMXJ010000000
VERSION     DATMXJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30239"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..>2167
                     /locus_tag="VN377_06690"
     CDS             130..>2167
                     /locus_tag="VN377_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HWR28105.1"
                     /translation="MEPEKIDLTGNFSFQTKEDLFTVFQTSPQGLPETVAKERLHIYG
                     TNEFAKIKKKTIYRKIIEALIEPMALILIIAALLSFFIINDALEAFAIVAVVLINTII
                     GLIQEGKAEKAAEELKKILSPQCKVIRGGNVDVIASKFLVPGDIITFESGDIIPSDAR
                     LIEATDLLVDEAHLTGESEPIQKNGQPILGKNLKLYEMKNIVFAGSKVLNGHAKALVV
                     KTGNITEIGKIAKNIQETEDERTPLQKKMDHEVKSLVALAIVALILVLVMGYLRGMDF
                     GFSILLAISILVAVFPEGLPASLTIALSLAMERLAKNSVIIKQLSSVETLGNVDYICT
                     DKTGTITKHDMTVKEFFIGIKFYVMADVFKMIAEGKSALLHDLFLTSVKCSTAQVVEQ
                     DGNVIKELGDPTEIALIKASILNGFKPDQFDSFNIVDSVPFSSELMFSAILTKDSLGK
                     QDIYIKGAPEKILMFCTSYYLDGKIQTLDDHHRQHIGKELSTRSEKGFRLIGFVKKSN
                     VGDIQKIDVNGLSGFTFLATAAIYDPPKDEVKQMIRETQEANINVVMITGDSLKTGFS
                     IAESVGIASDIDQAIEGKDLEQLTDEEFENRVEHLRVYSRVAPLDKLKIVEKLRAKNH
                     IVAMTGDGVNDAPALKKADVGIAMGRAGTQVSQEAANIILTDDNFSVIVKAVEEG"
BASE COUNT          673 a          429 c          480 g          585 t
ORIGIN      
        1 cccttatctt ccaactcttt aataatctat ggttttttct caagaaaacc aggatttttt
       61 tgctcgtatc aaaaactaat aaaaagaaga tatgatttca ggggggacaa tcaaaaggaa
      121 aaaaatagaa tggaacctga aaaaatcgat ttgaccggaa atttctcttt ccaaacaaag
      181 gaagatttat ttacagtgtt tcaaacctcg cctcagggtc ttccagaaac agttgcaaag
      241 gaacgtttgc acatttatgg aacaaatgag ttcgcaaaga taaaaaagaa aaccatttat
      301 cgaaaaatta ttgaagcact gattgaacct atggctctta ttctgattat cgctgcactt
      361 ctttcgtttt ttatcatcaa cgatgctctt gaagcttttg cgatcgtagc ggttgttctc
      421 atcaacacga tcattggtct gatccaggaa ggaaaagcag aaaaagcagc ggaagagttg
      481 aaaaaaattc tttcccctca atgtaaagtg attcgcggtg gaaacgttga tgtcatcgct
      541 tcgaaatttt tagtacccgg ggatatcatt acctttgaat ctggggatat tatccctagt
      601 gacgcacgac ttattgaagc gacagattta cttgttgacg aagcccatct caccggtgaa
      661 agcgaaccga ttcagaaaaa tggacaaccg atccttggaa aaaacctgaa gctctacgag
      721 atgaaaaata ttgtattcgc tggcagtaaa gtcctcaacg gtcatgcaaa agcactcgtc
      781 gtaaaaacag ggaatatcac agagatagga aaaatcgcga aaaatattca ggagaccgaa
      841 gacgaacgca cacctctcca aaagaaaatg gaccatgaag taaaatcgtt ggttgcactg
      901 gcaatcgttg ctctcattct tgtgctagtc atggggtatc tgcgagggat ggactttggt
      961 ttttcaattt tacttgcaat tagcatatta gtcgcggtat tcccagaagg attgcccgca
     1021 tccttgacca tcgcgttatc cctggcaatg gagcgacttg caaaaaactc cgtcatcatc
     1081 aaacagcttt catcggttga aaccctcggg aatgtcgatt atatctgcac tgataaaaca
     1141 ggtactatca caaaacatga tatgacggtg aaagaattct ttattggtat aaagttctac
     1201 gtgatggctg atgtgttcaa gatgattgcg gaaggaaaat cagcgcttct gcacgatctt
     1261 tttttaacct ctgtaaaatg ctctaccgcc caggtcgtcg aacaagatgg aaacgtcatc
     1321 aaagagttag gggatcccac tgagatcgca ttgattaaag catcgatttt aaatggtttt
     1381 aaacctgatc aattcgattc gttcaacatt gtcgacagtg ttccattttc ttctgagctg
     1441 atgttctctg cgattcttac aaaggatagt cttgggaaac aggacattta tatcaagggt
     1501 gccccggaaa aaatccttat gttctgcacc tcgtattatc tcgatggtaa gattcagaca
     1561 ctggatgatc atcaccgtca acatatcgga aaagaactga gtacacgatc tgagaaaggg
     1621 ttccgcctca ttggttttgt aaaaaaatca aatgttggtg atatacagaa aatcgatgtg
     1681 aatggtcttt caggattcac gttccttgct actgctgcta tctatgatcc tcccaaggac
     1741 gaagtaaaac agatgattcg ggagacacaa gaagcgaaca tcaatgttgt gatgatcacc
     1801 ggtgatagtt taaaaaccgg tttttccatc gcagagagcg ttggtattgc ctccgatata
     1861 gaccaggcga tcgagggaaa agatctggaa cagttgacag atgaggaatt cgaaaaccga
     1921 gtcgagcacc ttcgggtgta ttctcgtgtc gcaccgctcg ataaattaaa gattgtggaa
     1981 aaactccgag cgaaaaacca tattgtcgcc atgaccggtg atggtgtcaa tgatgcacct
     2041 gcattgaaga aggctgatgt cgggatcgcg atgggcaggg ctgggacaca ggtgtcgcag
     2101 gaagcagcga acatcatcct gactgatgat aatttttctg tcattgtcaa ggcggtcgag
     2161 gaaggac
//
LOCUS       DATMXJ010000259         6035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_72966, whole genome shotgun sequence.
ACCESSION   DATMXJ010000259 DATMXJ010000000
VERSION     DATMXJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72966"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..198
                     /locus_tag="VN377_06695"
     tRNA            127..198
                     /locus_tag="VN377_06695"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Gln,seq:ctg)
     gene            218..1903
                     /locus_tag="VN377_06700"
     CDS             218..1903
                     /locus_tag="VN377_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HWR28106.1"
                     /translation="MESTEQIIKDFIREIIDEDIKTNKYNGRVHTRFPPEPNGYLHIG
                     HAKSICLNFGIAEDYKGLCNLRFDDTNPTKEEQEYVQSIIDNVKWLGFDWEDRLFFAS
                     DYFTQMYEYALQLVKAGKAYVDDLPADQITEYRGNLTKPGKNSPYRDRPIEENLDLFQ
                     RMKSGEFADGQKVLRAKIDMAHPNLNMRDPVMYRTLHASHHRTGDAWCIYPMYDWAHG
                     LEDSIEGITHSICTLEFENHRPLYDWFLEALGVYHPQQIEFARLNLTYTVMSKRLLLQ
                     LVEGNHVCGWDDPRLPTISGMRRRGYSPSSIRMFCKRIGVAKFNSIVDFEFLEHNIRE
                     DLNKTSPRFMGVLHPLKVILENYPENQNEELEAVNNPEDPTMGTRRIPFSQTLFIEQD
                     DFMENPPKDYYRLAPGREVRLRYAYFIKCVDVVKKDGKPVELRCTYDPATKGGYAPDG
                     RKVKSTIHWVSAPHAVPTEVRLYDRLFTVTDPVGQKGVDFKEFLNPNSLEVLRDCQVE
                     PEIASLKPFDRFQFERLGYFCVDPTTTKNSLVLNRTVELRDSWAKLQKTAQKK"
     gene            1970..2040
                     /locus_tag="VN377_06705"
     tRNA            1970..2040
                     /locus_tag="VN377_06705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2002..2004,aa:Gln,seq:ttg)
     gene            2251..2370
                     /locus_tag="VN377_06710"
     CDS             2251..2370
                     /locus_tag="VN377_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HWR28107.1"
                     /translation="MHKATCADCGKECEVPFVPNGTKPVYCRDCYQQHRPKRF"
     gene            2500..3537
                     /locus_tag="VN377_06715"
     CDS             2500..3537
                     /locus_tag="VN377_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HWR28108.1"
                     /translation="MSVFTGFGSDNHSGVHPDILSALQNVNHGYVVAYGHDEYTTQAI
                     NRLKEIFGKESAVFFVYNGTAANILGLRSVTDSFHSIFCAESAHLTVHECCGPENYIG
                     TKLVNIPTPDGKLTVDLIDPYMIGVDDPHMAQPHVVSITQATERGTVYRPNEMQKLAD
                     FAHAHKMLVHMDGARLCNAAAFLKTGLHEISGGAGVDVLSFGGTKNGMMYGEAVVFFD
                     KNLAKNFEFIRKQGMQLTSKMRYISAQFSAFLAHDLWLRNASHANEMAQLLAEQLQSI
                     PGIEMSQKVEANMVYAIIPNQCIIRLQKQYYFHRFNERTCEVRLMCSFTTQPEDVLSF
                     AQAIRKAVGGT"
     gene            complement(3544..4410)
                     /locus_tag="VN377_06720"
     CDS             complement(3544..4410)
                     /locus_tag="VN377_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWR28109.1"
                     /translation="MNTKQLGVATILLASIMWAIEPILVKLSYENSDFLHTSAIRALV
                     AALTAFLYIALTKQSPLKVTRKQLPPLIYIALVGSLVADFMYYVALSQIPVINAVIIG
                     HMQPVFIIAIGFFLLKNDTLTKFDYLGIVLMILSGLLVTTGTLDNLVGLRFGTLGDLI
                     VLSATIAWATSAIVMKKYLTRMDAGMITFYRFFFPTLIFAAYLGVTSSFFIANIYQVC
                     IGVIIGVGTILYYEGLKRLKAAQASGLELSAPFFAAIFAFLFLNELTTGMQIIGIALL
                     FGGVYLISKKEK"
     gene            complement(4475..4720)
                     /locus_tag="VN377_06725"
     CDS             complement(4475..4720)
                     /locus_tag="VN377_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28110.1"
                     /translation="MNLLRIIKTLTHILLTIIIILFIITGFGITNYQIIESLTLGGLS
                     KPLSFQIHTNLILPLIILLVLHISFSIGNKIQKENHS"
     gene            complement(4717..5397)
                     /locus_tag="VN377_06730"
     CDS             complement(4717..5397)
                     /locus_tag="VN377_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWR28111.1"
                     /translation="MKKRSSMILILLSILILLPTSFTGCFEQQHIQLDAVEIREYQGE
                     KLSSVNDFRENSIKGPQYINTSTYQLKITGLVKNPKNYTYDEIIATFQHYKKVVTLDC
                     VEGWSVKILWEGILIKDLLNQTWPDQNATTIIFKAYDGYTTSFPLNYTTDNNILLAYK
                     MNNATLPPELGFPFQLVAESKWGYKWIKWVTEIDLSDNRNYKGYWESRGYSNSGDLNT
                     SFFEKWPL"
     gene            complement(5491..>6035)
                     /locus_tag="VN377_06735"
     CDS             complement(5491..>6035)
                     /locus_tag="VN377_06735"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWR28112.1"
                     /translation="RKAARRKSKAEVIMNTLNIRPGQTIADIGSGGGFFTFLFSQQVG
                     KDGKVYAIDTNQDFLEFIDKQAARHKLTNITTLLATKHAIPLPSHSIDLLFVRSVYHH
                     LQNRTFYFSEAKKVLTSQGRIAVIEYSQKGFKYSFHRRCGHNVPKEIIIDEMTKAGYK
                     VSASFDFLPVQSFTIFTPIT"
BASE COUNT         1652 a         1250 c         1467 g         1666 t
ORIGIN      
        1 gtacaatgtt aaatttatta aaaataaagg aaaaaagacc tcctcaccgt tctcatcaga
       61 aaactcggaa agatgaaaag ttatatatga aacatttctg cttccggttt cgattaacat
      121 agtcatagcc ccgtagtgca gcggttagca caagagactc tggatctcta gacaccagtt
      181 cgaatctggt cggggctgtc aatcgagtgt gaggatcatg gagtcaacag aacagataat
      241 caaggatttt atccgggaaa tcatcgatga agacatcaaa acgaacaaat acaacggccg
      301 ggtccatacc aggtttcccc cggagccaaa cgggtacctg cacatcggtc atgcgaaatc
      361 catctgcctg aacttcggta tcgcggagga ctacaaaggt ctctgcaacc tccggttcga
      421 tgacacgaac ccgaccaagg aagaacagga atacgtccag tccatcattg ataacgtcaa
      481 atggcttggt ttcgactggg aggaccgctt gttcttcgcc tcagattatt ttacccagat
      541 gtacgagtat gcattgcaat tagtcaaggc gggaaaagcc tatgtggatg atcttcctgc
      601 tgaccaaatc accgaatatc gagggaacct tacaaaaccc gggaagaaca gcccctatcg
      661 tgatcggcct atcgaagaga acctcgacct gttccagcgg atgaaatcag gggaattcgc
      721 tgatggtcaa aaggtcttgc gcgcaaaaat agacatggcg catcccaacc tgaacatgcg
      781 tgacccggtg atgtaccgga ccctgcatgc gagtcatcat cggaccggtg atgcatggtg
      841 catctacccg atgtacgact gggcgcatgg cctggaagac tccatcgaag gcatcaccca
      901 ttcgatctgt acccttgagt ttgagaacca tcgaccgctg tatgattggt tcctggaagc
      961 actcggggtg tatcatccgc agcagatcga gttcgcccga ttgaacctca cctatactgt
     1021 catgagtaaa cgactactcc tccagttagt ggaaggaaac catgtctgcg gctgggatga
     1081 cccacgcctg ccaaccatca gcgggatgcg tcgtcgcggc tattctccat catcgatcag
     1141 gatgttctgt aaacggattg gtgttgcgaa attcaacagc atcgtcgatt tcgagttcct
     1201 tgaacataac atccgggagg atctgaacaa gacctcacca cgatttatgg gagtcctcca
     1261 tccactcaaa gttatccttg agaactaccc tgaaaatcag aatgaagaac ttgaggcggt
     1321 caacaaccct gaggatccca cgatggggac ccggaggatc ccgttttcac aaacactatt
     1381 cattgaacag gatgatttca tggaaaaccc accaaaggat tattaccggc tcgcaccagg
     1441 ccgggaggtg cggctccgct acgcgtattt cattaaatgc gttgatgtgg taaaaaaaga
     1501 cggaaaaccg gtcgagctac gctgtacata tgatcctgcg acaaaagggg gatacgcccc
     1561 ggatggcaga aaagtcaaat ccacgatcca ctgggtttcc gcgccgcatg cggttcccac
     1621 agaagtacga ttgtacgaca ggcttttcac ggtcactgat cctgtgggac agaaaggtgt
     1681 agacttcaaa gaattcctca acccgaattc ccttgaggtg ctccgtgact gtcaagtcga
     1741 acctgagatc gcgtccctga agccgtttga tcgtttccag tttgaacgcc tcggatattt
     1801 ctgtgtcgac ccaacgacga cgaagaattc attggttctg aatcgaactg ttgagctgcg
     1861 tgactcctgg gcgaaacttc agaaaaccgc gcagaagaaa taaatcattt tcacagcttt
     1921 cagaatgcat atgcttaaat aaaatcaaag attcccggta tgagcatcta gccccgtggt
     1981 gtagtggcaa cattttggcc tttggagcca acgtcggcgg ttcaaatccg cccggggctg
     2041 ttttatcgat aaaagatttt tattaggttt atgttatggt taaacaacgt ctattctttc
     2101 tgtaaaaaac aagaaaccgc ttgttttttc cttctgattc gtcgaggtat acgtttatat
     2161 accgcatgat taatacaacc gcaatattag gaagtaataa atatgtatag agaagaagga
     2221 cgcggtcgtt atgggggcgc accacgagaa atgcataaag cgacctgtgc agattgtggg
     2281 aaagaatgcg aagttccgtt tgtaccaaat ggtacaaaac ctgtgtattg cagggactgt
     2341 tatcaacaac atcgcccaaa aagattttag taaaaaataa attctattta gattcattat
     2401 ttttttacta attctttttt tctttttttt attctactga cagacagtat acataatcag
     2461 atagaagatt ttttataaca attgctgctt gatgcatgca tgtcggtatt tacgggtttt
     2521 ggaagcgata accattccgg tgtacatcca gatattctca gtgcccttca aaacgtcaat
     2581 catggatatg tcgtagccta tggtcatgat gagtatacca cccaagcgat caacagactc
     2641 aaagagattt ttggaaaaga gtctgctgtc ttctttgtgt acaacgggac tgcagcaaac
     2701 atccttgggc ttcgaagtgt gactgactcg tttcactcca ttttttgtgc ggagagtgct
     2761 catctgacgg tccatgaatg ttgtggacct gagaattata ttggaacgaa actggtgaat
     2821 atcccaacac ctgatggaaa acttacggtc gatttgatcg acccgtatat gattggagtc
     2881 gatgaccctc atatggctca accgcatgtg gtttcaatca cacaggccac tgagcggggg
     2941 acggtgtacc gcccgaatga gatgcagaaa cttgctgatt ttgctcatgc gcataagatg
     3001 ttagtgcata tggatggtgc ccggttgtgt aacgcagcag cgtttttgaa gaccggttta
     3061 catgagatct ccggaggggc tggtgttgat gtcttgtcct ttggaggaac aaagaacggc
     3121 atgatgtacg gggaggcagt ggtgtttttc gataagaatc ttgcgaagaa ttttgagttt
     3181 atccggaagc aaggcatgca gctgaccagt aagatgaggt acatctctgc acagttctcc
     3241 gcgtttttgg ctcatgatct ttggttacga aatgcttctc atgccaatga gatggcccag
     3301 ctcctcgcag aacaactcca gagtatccct gggattgaga tgtctcaaaa ggtagaggca
     3361 aacatggtgt acgcaatcat cccgaatcaa tgtatcatac gtcttcagaa gcaatactat
     3421 ttccatcggt tcaatgaacg cacctgtgaa gtgcgactca tgtgttcgtt tacgacacaa
     3481 ccggaggacg tcttgtcgtt tgctcaggcg atacgaaaag cagttggtgg gacataaaag
     3541 gagttatttt tctttttttg agatgaggta cacaccacca aaaagcaatg cgatcccgat
     3601 gatttgcata ccggtagtaa gctcattgag aaagagaaac gcaaatattg ctgcaaaaaa
     3661 cggggccgat agctccagac ctgacgcttg tgctgctttc aaccgtttca acccttcata
     3721 atagaggatg gtaccaacac cgataatcac accgatgcac acctgataga tattggcaat
     3781 aaaaaatgat gatgtcacac cgagatatgc tgcaaaaata agggtgggga agaaaaagcg
     3841 atagaaggta atcatgcctg catccatcct ggtaaggtat tttttcatga cgattgcgga
     3901 ggtcgcccag gcgatagtcg cggataagac gataaggtct ccgagagtgc cgaacctcag
     3961 gccgaccagg ttatccaatg ttccggtggt gacgagcaaa ccggagagaa tcatcagaac
     4021 aatgccaagg tagtcgaact tggtgagcgt atcgtttttc agcagaaaaa atccgattgc
     4081 gatgatgaag accggctgca tatggccgat gatgacagcg ttgatcactg ggatttgaga
     4141 taacgcgaca taatacatga aatctgcaac cagtgacccg acaagtgcga tatagatcaa
     4201 agggggaagc tgttttctgg ttaccttcaa tggagactgt tttgtcaatg cgatgtagag
     4261 aaatgcagtg agtgcagcaa ccagtgctcg tattgctgag gtatggagga aatcagagtt
     4321 ttcgtaggag agtttcacca gtattggttc tatcgcccac atgatgctag caagcagtat
     4381 cgttgcgaca ccgagttgtt ttgtgttcac aagtgatgtt aaaaggtaat gagcttaaat
     4441 tgttttttct gatgcacgtt acaaacatgg aatattagga atgattctct ttctgaattt
     4501 tattgccaat ggaaaaggaa atatggagaa ctaacagaat gattaacggg aggattaaat
     4561 tggtatgtat ttgaaacgat agtggtttgg aaagaccacc gagcgtaagt gattcaataa
     4621 tctggtaatt ggtgatccca aatccggtga tgatgaaaag gatgatgata attgtgagga
     4681 ggatatgggt gagagtttta atgattcgaa gtagattcat agtggccact tttcaaaaaa
     4741 gcttgtgttc agatccccgc tgtttgaata tcctctgctt tcccagtatc ccttataatt
     4801 gcggttgtct gagagatcga tttctgtgac ccatttgatc catttgtatc cccatttgct
     4861 ctctgcgacg agttggaagg gaaacccgag ttctggtggg agggttgcat tgttcatctt
     4921 gtatgcaagc aggatgttgt tgtctgtggt gtagttcagg gggaaggaag tggtgtaccc
     4981 gtcatatgct ttgaagatga ttgtcgtagc gttttgatct ggccatgttt ggttcaaaag
     5041 atcctttata aggatgcctt cccagaggat tttgacgctc cagccttcca cgcaatccag
     5101 ggtgacgacc tttttgtaat gctggaatgt ggcgatgatt tcgtcatagg tatagttttt
     5161 tggatttttt accaacccgg ttattttcag ctgataggtc gatgtgttga tgtattgtgg
     5221 tcctttgatg gagttctccc gaaagtcgtt gactgaagaa agtttttctc cttggtattc
     5281 tcgtatttcg actgcatcga gctggatgtg ttgttgttcg aaacaccctg tgaatgaggt
     5341 gggaagaaga atcaatatac tgagaagtat gagtatcatt gatgatcttt ttttcattgt
     5401 gtggtctcct atcttcctaa ttaacaattg ttaatattag ggttgtggag gtatttaaca
     5461 atatcgttaa gcttcgtttc ctcccctttt ttacgtaatt ggtgtaaaga tagtaaagga
     5521 ttggactggc agaaaatcaa atgatgctga caccttgtac cctgctttgg tcatttcatc
     5581 gatgatgatt tctttcggaa cattatgacc gcatcgtcga tggaaactgt acttaaaacc
     5641 cttctgggaa tattcaatga cggcgattcg cccctgtgat gtcaaaacct tttttgcttc
     5701 tgagaaatag aaagtacggt tctgtagatg atgatatacg ctacgaacga agagaaggtc
     5761 gatggagtgc gatggcagcg ggatagcgtg ttttgtcgca agtaaagttg ttatgttcgt
     5821 aagtttatgt ctggctgctt gtttgtctat aaattcaagg aagtcttgat tcgtatcaat
     5881 cgcatagact tttccgtctt tccccacctg ttgtgaaaag aggaatgtga agaatccacc
     5941 gcctgatccg atgtctgcga tggtttgccc tggccggatg ttcaaggtat tcatgattac
     6001 ctccgctttg ctttttctgc gagcagcttt cctgt
//
LOCUS       DATMXJ010000260         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30258, whole genome shotgun sequence.
ACCESSION   DATMXJ010000260 DATMXJ010000000
VERSION     DATMXJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30258"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VN377_06740"
     CDS             complement(<1..530)
                     /locus_tag="VN377_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28113.1"
                     /translation="MDLALHLQSSKRNLLFSFLPTALMLSGVGFIIMFNPMDLSYVFH
                     YLFFGCLLLVVLIDYQYVLKGVDAPRLFKMKEPVALEMNEPGPPVSRAPSFFTKIKPT
                     QHPSAPLRHENAAEFQRISDAMVQKMQNVVNDLERKTERIEKLENILGQQQKHPHSIE
                     KIFPTQDVPSIDRSDK"
     gene            complement(718..1413)
                     /locus_tag="VN377_06745"
     CDS             complement(718..1413)
                     /locus_tag="VN377_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28114.1"
                     /translation="MRKAIASFFILFMAVLLCSVVLSLHANAGQAGVGVINVPPKYGY
                     IRVEEQGDYIRVYLNISDYNSWADVYNVSVTLDNYGVKQSMFLFQQYKNLDSFVEINE
                     FSETPKKRHLLDIEKCFFEKSNGQETIDERCDIAIRFVFQKTFFTGLSIVINDRTGSA
                     PAEAYIYYNSEESLRSGNTIVIPWIDVQITLTLPPYLIDLIAIILATIGVMYYLKKTG
                     IWEKRKEIYEKIS"
     gene            complement(1424..2773)
                     /locus_tag="VN377_06750"
     CDS             complement(1424..2773)
                     /locus_tag="VN377_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28115.1"
                     /translation="MGRQNNKQSIVFLILLLYFSTVLSTVSAAQDATLSIHMLQGEQQ
                     TRFNAPVLVSGIWHYVNITTAQSIDELSLRFYKGVLIPSGEKNETNYYEWNYNKNTAS
                     VWSDVSGYEIKYIQQDLCHDTNTMYSFCIGIKDFMPNIVDYYENWTAEVSSLGTMIHS
                     ETIVVEKPKTGVSLSKPSSIIFHVDPFTIMDAVGDNFFKIGNIGNIPFSINFDNERYS
                     DVEINDMNTKLLPEEIITHYVTVHSRSWPPGFKTIDIQLNGSYPRLYFVDTNATVTLY
                     TSFIIDVPQLIIYVGHSNYKIDELQGTGITFQYIENLNMYEGEIRDVTAYVSGNGAVT
                     AELWADEKNISILKLYDDGIETHSPLSFSSTNASERIIKVTVKALSEGTTGILTYRIT
                     ANGITKTYTTQISIGPPESPDDEDTGGSFSIMQLVVVGIVVLVLVYMIVTYLRNRKR"
BASE COUNT          866 a          593 c          652 g          919 t
ORIGIN      
        1 tccttatctg atctgtcaat tgaaggaacg tcctgagttg gaaatatttt ttcaatggag
       61 tggggatgtt tctgttgctg accaagtatg ttttccaatt tttctattcg ttctgttttt
      121 ctttccaggt cattgactac gttttgcatt ttctgaacca tagcatctga tattctttga
      181 aattcagcgg cattctcatg gcgaagagga gcagaaggat gttgagtagg ctttattttt
      241 gtaaaaaaag aaggtgctcg tgacacagga ggccctggtt cgttcatttc caatgctacc
      301 ggttctttca tcttgaagag ccttggagcg tctacacctt ttaagacata ctgataatca
      361 atgagaacaa caagtaacag acagccgaag aagagataat gaaagacata ggacaaatcc
      421 atggggttga acatgataat gaacccgaca ccggaaagca tgagggctgt tgggaggaag
      481 gaaaacagta aatttcgttt acttgactgg agatgaaggg ccaggtccaa aacgataact
      541 aaaagggaaa ccgagaggag ctgcacgtta ctgaatccgg ataacagata cagtggtgtg
      601 agttcgtaga tgaattgtgg ttcatagctg gctttgaaga agaagatgaa gataaatttt
      661 acaagcagat agattgagaa aaatataaag aaacagtttg gaaaaagcca tgaccgacta
      721 tgagattttt tcgtagattt cttttctttt ttcccagatc cctgttttct taagatagta
      781 catcacacca attgttgcta gtattattgc aatgagatcg atcagatacg gagggagagt
      841 cagagtaatt tgtacatcta tccatgggat cactatcgta ttaccgcttc tcagggactc
      901 ctcactgttg taatagatgt atgcctccgc cggagcagat ccggtccgat cattaatcac
      961 aatgctgaga ccagtaaaaa aagttttttg aaaaacaaag cgtatcgcta tgtcgcagcg
     1021 ttcatcgatc gtttcctgtc catttgattt ttcgaaaaaa catttttcga tatccaatag
     1081 atgccttttt ttcggtgttt cgctgaactc gttgatctca acaaaggagt ccagattctt
     1141 atattgctgg aaaagaaaca tggactgctt gaccccataa ttatcaaggg tcacgctcac
     1201 attgtaaaca tcagcccagg agttataatc agagatattg agatagactc gtatgtagtc
     1261 accctgctcc tcgacccgga tgtaaccata ttttggggga acgtttatga cacctacacc
     1321 tgcttggcca gcgtttgcgt gcaacgacag aacgacagag cagaggagaa cagccataaa
     1381 cagtatgaaa aaggatgcaa tagctttcct catttttctt ctgttacctc tttcgatttc
     1441 gtagatatgt cacaatcata tagacaagaa cgagaacaac gatgccgacg acaacgagtt
     1501 gcatgatgga aaaagatcct ccagtgtctt catcatcagg ggattctggc ggcccgatgc
     1561 tgatctgtgt cgtgtatgtt tttgtaatac catttgcggt gattcgatac gtaagaatac
     1621 cggttgttcc ttcactgagt gcttttacgg tcaccttgat gatccgctct gaggcattcg
     1681 tggaactaaa cgaaagaggg gagtgtgttt caataccgtc gtcatagagt ttcagtatgc
     1741 tgatattttt ttcatctgcc cagagctctg cggtgactgc cccgttacca gaaacatatg
     1801 cagtgacgtc ccgtatctct ccttcataca tgtttaaatt ttctatgtac tggaaggtga
     1861 ttcctgtccc ctggagctca tcgatcttgt aattgctatg cccaacatag ataatcaact
     1921 gcggcacgtc aatgataaag gaggtgtaca gggtgacggt ggcattggtg tcaacgaaat
     1981 acaaccgagg atacgaaccg ttcaactgga tatcaatcgt tttaaaccca ggtggccaac
     2041 tccttgaatg aaccgtaaca taatgagtga tgatttcttc tggtaacaac tttgtattca
     2101 tatcattaat ttctacatca ctgtatcttt cgttgtcaaa attgatggaa aaagggatat
     2161 tcccgatgtt tcctatcttg aagaaattat caccgaccgc gtccatgatg gtgaacggat
     2221 caacatgaaa gatgatggat gatggtttgc ttaaggagac accggttttt ggtttttcta
     2281 caacaatggt ttctgaatga atcattgttc caagagagga cacttcagcg gtccagtttt
     2341 cataataatc aacgatgttt ggcatgaaat cttttatgcc gatgcagaaa ctatacatcg
     2401 tgttcgtatc atgacacaag tcctgttgaa tatacttgat ctcatatccg ctgacatcgg
     2461 accagaccga agcagtattt ttgttatagt tccattcgta atagtttgtt tcatttttct
     2521 ctccgcttgg aatcagtact cccttgtaga atcgaaggga taattcatcg atgctctgag
     2581 ctgtggtaat gttcacataa tgccagatgc ctgatacgag cactggtgcg ttgaagcggg
     2641 tttgttgttc cccttgtaac atgtgtattg aaagagttgc atcctgcgct gctgatacag
     2701 tggaaagtac tgttgagaaa taaaggagaa gaattaaaaa aacaatactc tgcttgttat
     2761 tttgtcgtcc cacttatccc tctcctgtaa ttagtgtaaa ttgcgcaaat acccatccgt
     2821 atttcttatc taatttcatc tattaaaggt tttctttgtg taatgtcaat agacatcgct
     2881 aacattcgaa ataaaatgat gaaggtattc cctccacccc gagggaatat cgcactgtgt
     2941 tgaaccattc tatatattca tccagaagaa gggatgcatt ttcaaacacc ttgtccagga
     3001 tgagatactc ttctttgaga ttgcgaatcg
//
LOCUS       DATMXJ010000261         8733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_9897, whole genome shotgun sequence.
ACCESSION   DATMXJ010000261 DATMXJ010000000
VERSION     DATMXJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9897"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VN377_06755"
     CDS             <1..440
                     /locus_tag="VN377_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWR28116.1"
                     /translation="KGPFLLDGKRLYALIIVDDHSRFIIYCRLYSSIRKEEVVTALHE
                     AVKHYRHPRRLLCDNGSQFKSNDLKCWCKTHKVLYEPAPPHYPETKGKVERAIRNLKE
                     EYLILDKVFENASLLLDEYIEWFNTVRYSLGVEGIPSSFYYEC"
     gene            552..1457
                     /locus_tag="VN377_06760"
     CDS             552..1457
                     /locus_tag="VN377_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28117.1"
                     /translation="MNRNPLIRKCLVIGIIISFLEISIIPSVAKPEGIPVSFVPPQEI
                     FFGLQTNINISWDENLTKEIDLEHSPPIVPLNVSFWVTWGLFGRLINLFYSYQKLNAN
                     FLIDNSPWSSSSLTFNQLPLYLPPKENIQQISMNQLTIDLDYWTPAYELIPITIETRI
                     EPILGPSGSLVLIRGTTYRETIYVMAQYRSGLGINYPEGQTFETPPLIQVEVPLEIYN
                     HGNGKTLVESEVDDSPPGWNISLPSQLILEQQEEAEILVTLMAPSDFSGYESVWLDFT
                     PHYYYNYSVVGYTYSAHISVMYNPP"
     gene            1734..3023
                     /locus_tag="VN377_06765"
     CDS             1734..3023
                     /locus_tag="VN377_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWR28118.1"
                     /translation="MSILIKNSTILTQNDSRRQIHGDIYIDDHVIVECSEKPISLEAD
                     FKIDGTHKLVLPGLINTHTHIPMTLFRGYGDDMVLGDWLAQRIWPVEAKLDKKAVEAG
                     TELGLLEMISSGTTSYLDMYFFEETIAKVTERVGMRGFLGFALIDFDTPEYDAGELIP
                     QCEQFVRRWKNNPLISPVIAPHSTYSCKPETLQKALDIATRHHVPLHTHCSETRDEVY
                     DIEKKYGVRPVEQLKKHGLLQKDTILAHCGWITKNEILDMKTAGVSVSHCPVSNMKIA
                     TGGFAPVPELLDAHVPVGLGTDGAASNNTLDMFETMKFCALVHKNHRWDPTVLPAQTV
                     VDLATLGGAACLGMRHALGSVEEGKTADLIMIDLKKPHLTPLHDPVSHLVYAVRGTDV
                     CTTIVNGTPLMLEKEFLTIDVEKTLGLAEKIASELTS"
     gene            3049..4311
                     /locus_tag="VN377_06770"
     CDS             3049..4311
                     /locus_tag="VN377_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HWR28119.1"
                     /translation="MLNLIGVILAFIIIILLIRKKVNFGLSLFLGSLIVGVFSLQLIT
                     PLDIPKAIIEASFYSFEKQEIITETIELALLMTLIFILAKTMQETGAITKLIDSLRTF
                     FSKGGTLAVIPAVYGLMPVPGGALFSAPLIDKEGDKYKLKSNQKNFLNIWFRHIWFPI
                     YPISSAMILICSVKFSGIPIQMLMLANIPSFLGVLLIGFFFLRRYTKSAPEIIGTVKK
                     EYHGLMFILPPIVPLLFYPLTFVGLSETRCFLIGVSLSLVLLYLLIHIDVRTYARIMK
                     KSFTWNLVFAIFGIMILREMIQISQIHVLITETMQNLAFPALFIVILIPLLLGTLTGY
                     NLGAVALSYPLVESFFIFTGIQPLGLTSLIFMSSLIGYLISPIHLCNVLSSEYLKTDT
                     TRMYKMFLPAAVILLCIQAVFVILAFPA"
     gene            4449..6041
                     /locus_tag="VN377_06775"
     CDS             4449..6041
                     /locus_tag="VN377_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWR28120.1"
                     /translation="MNQGMLFDHLNNPKFYGPHVTSVQLLQTHISYVALTGTYAYKIK
                     KPVNFGFLDFSTLDKRKYFCEEELRLNKRLCPEMYLDVLPITKRDNTLTLDGDGTIVE
                     YALKMKEFPQECIMTNMLQQGKISEEIIDHLITILIDFYTTQEPTEEITNYGALPSVK
                     QNIDENFEQTRPMIDITVPKETFLYVKDEVATFFERKKEAFERRMKEGKIYDCHGDLH
                     SGNIVVSGESIHIFDCIEFNDRFRFCDVASDVGFLAMDLDYLNHPYLSSYFIEKYVEK
                     SKDPGVLSMLNFYKSYRAFVRGKVHGFQLNDPHIDPITKKRLLASAKKYFELSQYYTR
                     LFSLDLKKNKPLIFLVTGLSGTGKSTVARKISVDYHATLINTDIVRKEAAGIDRYEQH
                     HDQYNTGLYDSKRIDGTYEQVMQRASQSLKKGENVVLDATFQKKKYREMASHIAGKHH
                     AAVVIVQCICPDAIVKKRLEDRVRKKSVSDGRWEIYQVQKTTFEPFTADERCHTIDTS
                     NDSYDFRMSFFQQLLAHVHEVV"
     gene            6041..7060
                     /gene="dph2"
                     /locus_tag="VN377_06780"
     CDS             6041..7060
                     /gene="dph2"
                     /locus_tag="VN377_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HWR28121.1"
                     /translation="MEISGYIVDVESATQVIIQNHYKTIAVQVPEGLKRSIFPLVDFI
                     RKETGATVLVSADPCFGACDVVNYELKNMDVDCVIHIGHTAIADVENFWIPTIFVNAQ
                     STLDVSKVVEKAIPSLIGKKIGLVTTAQHLHTLETVEKIIRQHGLEPLITEGDERITS
                     RGQILGCNFSAGANLTEQVDCFLFIGSGTFHPVGLLMSTKKPVIAADPYTNTVKKQEI
                     EDMKNNILRQRYGAILACRNARRVGILIGLKRGQQRIKQAYEVQQMLDAAGKLSLLIT
                     QDEFSPVSLQGFDLDCYVSTACPRIAIDDYLQYKKPIITPLELEVALGRREWETYVFD
                     EILKG"
     gene            7106..7759
                     /gene="radB"
                     /locus_tag="VN377_06785"
     CDS             7106..7759
                     /gene="radB"
                     /locus_tag="VN377_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HWR28122.1"
                     /translation="MTLLSLGCHPLDALLGGGLESGIITRIYGEAGSGKTNFCLQAAR
                     QCVSSKKKVAYIDTEGVSFERIRQICVDCDYKDILHDILFFTPTSFASQEQMICDAVA
                     MKGVGLIVVDTITMFYRLNLEEDREGSIRSFTRQVTNLQLAAREKDICVIITEQVYTN
                     KKGEVRPFTNRDAEHMTKTILRFDKKGIGEREAIIIKHRSQPEGKKASFRISTTGLE"
     gene            7889..8482
                     /locus_tag="VN377_06790"
     CDS             7889..8482
                     /locus_tag="VN377_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28123.1"
                     /translation="MDEHEDINYKTLRRIQQDEQTLSLLTKITVGFYKDLSSYMKTIE
                     RSIEHEKNPLKLKLFGDEIQNTRKIANSIYELREKKIVQAALATARGATPDLTNFLEI
                     EKKLYRSLVEQIMVSRKEIFEKPTDPPTATPPASPTPAPVKEDSNTHPIVRVLEDTPE
                     FIGTDGKSYLLRKEDVLSMPSEMTEPLLKKKVVKQVK"
     gene            8542..>8733
                     /locus_tag="VN377_06795"
     CDS             8542..>8733
                     /locus_tag="VN377_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HWR28124.1"
                     /translation="MKIPRKIKRYCPYCKKHTLHEVDKMKKRKASELKRGQRRFRRAT
                     SGYGGFPRPKPEGREKTSRR"
BASE COUNT         2592 a         1891 c         1831 g         2419 t
ORIGIN      
        1 tcaaaggacc atttcttctg gatggaaaac gactctacgc tctgatcatc gtggatgacc
       61 attcccggtt catcatctat tgcaggctgt actcctcgat tagaaaggag gaggtcgtca
      121 ctgctctcca cgaggcagtg aaacactatc gccatcccag acgactcctc tgcgacaatg
      181 gatcccaatt taaaagcaac gacctcaaat gctggtgtaa aacacacaaa gtcctctatg
      241 aaccagcacc ccctcattat ccggagacca aaggaaaagt tgaacgagcg attcgcaatc
      301 tcaaagaaga gtatctcatc ctggacaagg tgtttgaaaa tgcttccctt ttactcgatg
      361 agtatattga atggttcaac acagtgcgat attccctcgg ggtggaggga ataccttcat
      421 cattttatta cgaatgttag cgatgtctat tgacattaca gtctcaaaag tctaataact
      481 atataaaaac gtcaaattac gagtattttt ataggagaac cttgttataa aattataaag
      541 gaggaaaaat aatgaacaga aatccattga ttagaaaatg tttagtgatt ggaatcatca
      601 tctcattcct tgaaataagc attattcctt cagtggcaaa acctgagggc atccccgtaa
      661 gttttgtccc acctcaagag atattttttg gtttacaaac caatataaat atttcttggg
      721 atgaaaattt aacaaaagag atcgacttag agcattcccc gcctatagtt ccgttgaatg
      781 tttcattttg ggtcacctgg gggctttttg gtcgtttgat taacctcttt tattcgtatc
      841 aaaaacttaa tgcgaatttt ctgattgata attcaccctg gagttcttcg tctcttacat
      901 ttaatcaatt acctctctat ttacctccaa aggaaaacat ccaacaaata agcatgaatc
      961 aactgacaat tgacttagat tattggacgc ctgcctatga attgattcca ataacaattg
     1021 aaactaggat tgaaccgata ttagggccat ccggttctct tgttcttata cgcggcacga
     1081 catatcgcga aacgatttat gtgatggctc aataccgttc agggttaggt attaattatc
     1141 ctgaaggaca aactttcgag acgccaccgc tcatccaagt ggaagttcct ttagaaatat
     1201 ataaccatgg gaacggaaaa acactcgttg aaagcgaagt cgatgattcg ccacccggtt
     1261 ggaacatatc cctaccatcg caattaatat tggagcaaca agaagaagca gagatattgg
     1321 ttactctaat ggcaccgagt gatttctcgg ggtatgaatc tgtttggttg gattttactc
     1381 cgcattatta ttacaattat tcggtagtgg gatacactta ttctgcacat atctctgtca
     1441 tgtataaccc accataaatt gatattttat atcaatatat taagatggaa tgaaaaaaag
     1501 ggataatcgc ggtataatgg aatgtttagc ggttggaatc attctctttt gaaataaagc
     1561 ttaggattaa tcaattctaa tcaccgcccc ctcgcatttg taatcactca aataagaaca
     1621 ttcaaaaaca cacaaaacat ctacacagca gcaataacag aaaaaagtca cttaaaattt
     1681 aaattgtcat aaacccacaa tatcatcatt atctataagt ggagatatta agaatgagca
     1741 ttctcattaa aaattccacg attctaaccc agaatgattc tcgacggcag atccatggtg
     1801 atatctacat tgatgatcat gtgatcgtag aatgttcgga gaaacctatt tctctggaag
     1861 ctgattttaa gattgatgga acacataaac ttgtgttacc tggtctgatc aacacccata
     1921 cccatatccc catgaccttg tttcgcgggt acggagatga tatggtccta ggggattggc
     1981 ttgctcaacg gatctggcca gttgaagcaa aacttgacaa aaaagccgtt gaggctggga
     2041 cagaactagg ccttctcgag atgatttcgt caggcacgac atcatacctt gacatgtatt
     2101 tttttgagga aacgatcgcc aaagtcaccg aaagggtcgg gatgcgtgga ttccttggct
     2161 ttgcccttat cgattttgat accccagagt acgacgcagg agaacttatc ccgcaatgtg
     2221 aacaatttgt acgacgctgg aagaacaatc ccttgatttc acctgtgata gcaccgcata
     2281 gtacgtattc ctgtaaacca gagactcttc agaaagccct tgatattgcg acgagacatc
     2341 atgttccctt gcatacccat tgctctgaga cacgggatga ggtgtacgac atagaaaaaa
     2401 aatatggggt acgacccgtg gagcaattaa aaaaacatgg gttgttgcaa aaagatacga
     2461 ttcttgcgca ttgtggatgg attacaaaaa atgagatact ggatatgaaa accgcaggtg
     2521 tgtcagtatc ccattgcccg gtgagtaaca tgaaaattgc gaccggtggc tttgcgcctg
     2581 tccctgagct tctcgatgca catgtccctg ttggtcttgg gaccgatggt gcggctagta
     2641 acaataccct tgatatgttt gagacgatga agttctgcgc tcttgtccat aagaatcacc
     2701 gttgggaccc gacagtcctg cctgcccaga ccgttgtcga tctagcaact cttggtggtg
     2761 ccgcctgcct tggcatgaga catgctcttg gttctgttga ggaaggaaaa accgctgacc
     2821 tcatcatgat tgatctaaaa aaaccgcatc tcaccccgct tcatgaccca gtgtcccacc
     2881 ttgtctacgc tgtacgagga acggatgtgt gtaccaccat cgtgaatggt acaccactca
     2941 tgcttgagaa agaattcctg accatcgatg tggaaaaaac attgggactt gctgagaaaa
     3001 tcgcttcgga gttaactagt taaatccaac gattatttct ggtgtgtcat gctgaatctt
     3061 atcggtgtta ttcttgcgtt catcatcatt attctcctca tccgaaaaaa ggtgaatttt
     3121 ggtttatcct tgtttcttgg ttccctaatc gtcggtgttt tttctcttca acttatcaca
     3181 ccactcgata tcccgaaagc aataattgaa gcaagtttct attcgtttga aaaacaagaa
     3241 atcattacag agacgataga actagcattg cttatgacat tgatcttcat cttagcgaaa
     3301 acaatgcaag aaacaggggc aattacaaaa cttattgata gtctccgaac gtttttctct
     3361 aaggggggaa cactcgctgt gatccctgcg gtctatggtt taatgcctgt accaggggga
     3421 gcattgtttt ctgcgccact cattgataag gaaggagata aatacaaact gaagagcaat
     3481 cagaagaatt ttttgaatat ctggttccga catatttggt ttcccatcta tccgatttca
     3541 tcagcgatga tactgatctg tagcgtgaaa ttctccggca tccccatcca gatgctcatg
     3601 cttgctaata ttccttcatt ccttggggta cttctgattg gatttttttt ccttcgacgg
     3661 tatacgaaaa gtgctcctga gataatagga actgtcaaga aggagtatca cggattaatg
     3721 tttatcttgc caccgattgt ccctcttctg ttttatccat taacatttgt tggtctctca
     3781 gaaacacgat gtttcttaat cggtgtttca ttgagtcttg ttttgttata tctcctcatt
     3841 catatcgatg tgaggaccta tgctcgtatc atgaagaaat cgtttacgtg gaacttagtg
     3901 tttgcgatat tcgggatcat gatccttcgg gagatgatac agatttctca gatacatgta
     3961 ttgatcacag aaacgatgca aaacctcgct ttccccgcac tgtttatcgt gattctcatt
     4021 ccacttctcc ttgggactct cactggttat aatcttggag ccgtcgctct ttcctacccg
     4081 cttgtcgaat cttttttcat cttcaccgga atacagcctc ttggtttgac aagccttatc
     4141 ttcatgagct ctctgatagg gtatcttatc tcaccaatcc atctctgcaa tgtcctaagt
     4201 agtgaatatt taaagactga tacaactcgg atgtacaaga tgtttctccc tgctgcggtt
     4261 attctcttat gcattcaagc cgtttttgtg atccttgcat ttcccgcata aattatattt
     4321 tttttgtaca ttccactaga aaatactagg gaaccatcac tctattctac tttgatctct
     4381 gtgttgaggt atcaagtgac ctttttggac gacaagtctt ataaaaagtc agggtctacc
     4441 acatgggtat gaaccaggga atgctttttg atcatctgaa taatcccaag ttttatgggc
     4501 ctcatgtgac ctcagttcag ttgctacaga cccatatctc ctacgtagcc ctcacaggaa
     4561 cctacgcgta caagatcaaa aaaccagtga acttcggatt cctcgatttt tcaaccttgg
     4621 ataaacgaaa atacttctgc gaagaggagc tgcgactgaa taaaaggctc tgccctgaga
     4681 tgtatcttga tgttctcccc atcactaaaa gagataacac ccttactctt gatggggatg
     4741 gaaccatcgt cgagtacgca ttaaaaatga aagaattccc tcaggaatgc atcatgacaa
     4801 acatgcttca acaagggaaa atttctgagg agatcatcga ccatctcatt acgattttga
     4861 tcgattttta taccacgcaa gaaccaaccg aagaaatcac caactatggg gcattaccct
     4921 cggtcaaaca gaacattgat gagaatttcg aacaaacccg gccaatgatc gacatcacgg
     4981 ttccaaagga gacatttttg tacgtcaagg atgaagtcgc tacgtttttc gaacggaaaa
     5041 aggaagcgtt cgaacgccga atgaaagaag ggaagatcta cgattgtcat ggggatttac
     5101 actcagggaa catcgtggta tcaggtgaat ctatccatat ttttgactgc atcgagttta
     5161 acgaccgctt ccggttctgc gatgttgcct cagatgtcgg gtttttagcg atggacctcg
     5221 attacttgaa tcatccgtac ctgtcaagct atttcattga aaaatacgtg gaaaaaagca
     5281 aggaccctgg tgtgctttcc atgctgaatt tttacaaatc ataccgagcg ttcgtccgtg
     5341 ggaaagtcca tgggttccag ctcaatgacc cccatataga cccaatcacg aaaaagcgtc
     5401 ttttagcatc agcgaaaaaa tattttgagt tatcacagta ttatacaagg ttattttccc
     5461 ttgatctaaa gaaaaacaaa ccattgattt tccttgtcac tggtttatct ggaaccggga
     5521 aatcgacggt ggcacgcaaa atctcggttg attatcacgc gacactcatc aatactgata
     5581 tcgtgcgaaa agaggcagcg gggatcgatc gttatgagca acaccacgat cagtacaaca
     5641 ccggcctata tgactcaaaa aggatcgatg ggacctacga acaggtcatg cagcgcgcat
     5701 cacagtcctt aaaaaaaggg gaaaacgtcg ttcttgatgc gacattccag aagaaaaaat
     5761 accgggagat ggcatctcat atcgcgggaa aacatcatgc cgcggtggtt attgtacagt
     5821 gcatctgccc tgatgcaatc gtaaagaaac ggttggagga tcgagtgagg aaaaaatctg
     5881 tttccgatgg ccgctgggag atctatcagg tacaaaaaac aacatttgaa ccatttactg
     5941 ctgatgaaag gtgccatacg atagatacct cgaatgactc ctacgacttt cggatgagct
     6001 tctttcagca actccttgct catgtacacg aggtggtata atggagattt caggctatat
     6061 cgtcgatgtg gaatctgcga cgcaagttat tatacaaaac cactacaaaa caatcgcggt
     6121 gcaggtccct gaaggattga aacgcagtat ttttccgctt gttgatttca tcagaaaaga
     6181 gacaggtgct accgttcttg tgtctgcaga cccttgcttt ggcgcttgtg atgtggtgaa
     6241 ctacgaacta aaaaacatgg atgtcgactg tgtcattcat atcggtcata ccgcaatagc
     6301 ggatgtcgaa aatttctgga ttccgaccat atttgtaaac gcgcaatcaa cactggacgt
     6361 ttccaaggtt gtcgaaaaag cgatcccctc cttgatagga aaaaaaatag gtctcgtcac
     6421 caccgcgcaa catctccata cccttgagac tgttgaaaaa attatacgac aacatggtct
     6481 tgagccgctc atcactgaag gcgatgagcg catcacatca aggggtcaga tcctcggctg
     6541 taatttttct gctggggcga acctcacaga acaagtcgat tgttttttat tcatcgggag
     6601 tggaacgttt cacccagttg gtcttttgat gagtacaaaa aaaccggtga tcgctgctga
     6661 tccttatacg aataccgtga aaaaacaaga aatcgaggat atgaaaaata acatattacg
     6721 acagcgttat ggcgcgatcc tcgcgtgccg gaatgcacga cgagttggaa ttctcatcgg
     6781 gttaaaacgt ggtcaacaac gcataaaaca agcctacgag gttcaacaga tgcttgatgc
     6841 tgccggtaaa ctttccctgc tgatcaccca ggatgagttt tcccccgtat ctctccaagg
     6901 gttcgatctc gattgttatg tgtcaaccgc ctgtccacgg atagccattg atgattatct
     6961 ccaatataaa aaaccgatca taacccctct ggaactggag gtagctctgg ggagacgcga
     7021 atgggagacc tatgtttttg atgaaatact caaaggttga ttttccaaag gttctttatc
     7081 ttcctgttcc ctcagggtgt ctgtcatgac gctgttatct ctcggatgtc accctttaga
     7141 tgctttgctt gggggtggtc ttgaaagcgg aattatcaca cggatctacg gtgaggccgg
     7201 atcaggaaaa accaattttt gccttcaggc agctcgtcaa tgcgtttctt ctaaaaaaaa
     7261 ggtcgcatat attgataccg agggtgtttc gtttgagagg atacggcaga tctgtgtgga
     7321 ctgtgattat aaagatattc tacatgacat cctgttcttc actccgacat cgtttgcatc
     7381 acaagaacag atgatttgcg atgcggtcgc gatgaaaggg gtaggtctta tcgttgttga
     7441 tacgattacg atgttttatc gattgaatct tgaagaggac agggaaggat cgatacgttc
     7501 gtttaccaga caagtcacca accttcagct cgctgcccga gaaaaggata tctgtgtgat
     7561 tatcaccgag caggtttata caaataagaa aggtgaggtt agacctttta cgaaccgtga
     7621 tgctgagcat atgacaaaaa cgattttacg gttcgataaa aaaggaatag gggagcggga
     7681 agcaatcatc ataaaacacc ggtcacaacc cgaggggaaa aaagcgagtt ttcggattag
     7741 tacgactggt ttggaataaa gtttgaccat ctacagtcta tggccccgat cctttagctt
     7801 tcatacttat ttacagtcaa tacgtgacac gagtaatcta aggcagagct aaaactattt
     7861 aacatcagtt aagatgtaac gaacaagtat ggatgaacac gaggatatca actataaaac
     7921 actgcgtaga atccagcaag atgaacaaac attatctctt cttacaaaaa tcactgtcgg
     7981 tttctacaag gatctttcgt cgtatatgaa aaccattgag cgaagcattg aacatgaaaa
     8041 gaacccattg aaactcaagc tcttcggcga tgaaatccaa aacacgagga aaatcgcaaa
     8101 cagcatctat gagcttcggg agaaaaaaat tgttcaggct gctcttgcga ccgcgcgtgg
     8161 cgctacccct gatctgacga actttcttga aatcgaaaaa aaattgtacc gttcccttgt
     8221 tgaacaaatc atggtctctc gtaaagaaat ctttgaaaaa ccaacggatc cgcctaccgc
     8281 gacaccacca gcatcaccaa cacctgctcc tgtgaaagaa gattcaaata cccaccccat
     8341 cgtccgggtc ttagaagaca cccctgagtt tattggcacc gatggaaaat cctatttgct
     8401 gcgcaaagaa gatgtgcttt caatgccgag tgaaatgaca gaaccactcc tgaaaaaaaa
     8461 ggtggtcaaa caggtgaaat aaggcaccct acatttttat atacctttct tacttacggt
     8521 tacaatcgaa ctgtgatatc tatgaagata ccaaggaaaa tcaaacggta ctgtccttac
     8581 tgtaaaaaac acacgcttca cgaagttgac aaaatgaaaa aacggaaggc aagtgagctg
     8641 aaacgaggac aacgacggtt cagacgagcc accagcggat acggagggtt ccctcggcca
     8701 aaacctgagg ggcgagagaa gaccagtaga cgg
//
LOCUS       DATMXJ010000262         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30335, whole genome shotgun sequence.
ACCESSION   DATMXJ010000262 DATMXJ010000000
VERSION     DATMXJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30335"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1163
                     /locus_tag="VN377_06800"
     CDS             204..1163
                     /locus_tag="VN377_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HWR28125.1"
                     /translation="MDRIELLTHIREFLGSAGFSVSDQCALRLPGFDLVARRGETLLI
                     IKVLSNIDGLSEDVANEMRALAYLLKATPLLIGEKNGMNVLEDDVAYFRFGIQTVTAT
                     TLQNHLLEEIPVRAYAAPGGLYVNLDHEKIRRLRQEKNISLGSFAHHVRVTRRTVRMY
                     EDGMSARIDIADRIEEMFEQTVTLPIDLLKPLLMETKQLPFYKKDQGDTKELQSEIFT
                     LLQNIGYRVIPMGRCPFEALSKEKEKILLTCVQEYNKKLAEKAHFISSISKITKKHAV
                     VFTDKDVEKKNLEGTPIIGKKELRKIRDPEDVFTLILERITSD"
     gene            1268..1912
                     /locus_tag="VN377_06805"
     CDS             1268..1912
                     /locus_tag="VN377_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWR28126.1"
                     /translation="MADSKTVEAVQKAIEESKKLERKFKQSVDVVINLKNIDMNDAKN
                     RIEDEVMLPHGRGTEAKLALFASGDLALKSKNHVDLLIKPEEIEELAKDKKKFKKIAD
                     EYDFFIAEAPLMPTIGKTLGIILGPRGKMPKPVPPNIDIAGVAKSLRKTVKVRSKTTK
                     TIHATVGREEMNTEHIAENIDAILKRLEGKLERGKLNIGSVYVKTSMGPPMRIV"
     gene            1925..>2466
                     /gene="rplJ"
                     /locus_tag="VN377_06810"
     CDS             1925..>2466
                     /gene="rplJ"
                     /locus_tag="VN377_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWR28127.1"
                     /translation="MAHVASWKHGEVTEIISLMTHHKIIGIIDIGGIPAPQLQQMRGG
                     IRAKAQIRASKNTLILKALDDAESQIKGISALKNEVHGQAAIIATDMNPFALFREIKA
                     TRKNAPAKGGEIASHDIEVLAGDTPFKPGPIVGELQKAGIPAAISEGKVVIKSDKVLV
                     EAGQKISRELAQMLSRLDIFP"
BASE COUNT          763 a          566 c          590 g          547 t
ORIGIN      
        1 ggtcttccag gggacattcg ggttcagacg gacgagccgt gggtacccaa tgatttcgta
       61 gtcctgtatg attttttgta tgatggtccg cgccacatac gtggtacacc caccgcgtac
      121 agaatcagtg tcgtcaatgc ctatccagat gatgattcac caaaaacgtt attagagagg
      181 tcgtacttta agcttcatta cctatggata gaatcgaact acttacccac atcagagagt
      241 ttttggggag cgcgggtttt tctgtctcgg atcaatgcgc gttacgtctc ccaggtttcg
      301 acctcgtggc acgacgagga gaaaccctgc tcatcatcaa agtcctctca aacattgatg
      361 gactctccga ggatgttgcg aatgagatgc gtgcactagc gtatctgctc aaagccacac
      421 cgttacttat cggagaaaag aacgggatga acgtccttga ggatgacgtg gcgtatttca
      481 ggttcggcat ccaaaccgtg actgcaacaa cattacaaaa ccatttactt gaggaaatcc
      541 cggtgcgagc ctatgcagca cccggcgggt tatacgtcaa ccttgaccac gagaaaatac
      601 gacgattacg tcaggagaaa aatatctcac taggcagttt tgcgcaccac gttcgcgtca
      661 cacggcgcac cgtccgcatg tacgaggatg ggatgagtgc gcgaatcgat atcgctgacc
      721 gcattgagga gatgttcgaa cagacggtga ccctgccgat tgacctcctt aaaccactgc
      781 tcatggaaac caaacagtta ccgttctaca aaaaagacca gggggacaca aaagaactac
      841 aatcggagat attcacgttg cttcagaaca tcggttatcg tgttatcccc atgggtcgct
      901 gcccctttga ggcgctcagc aaagaaaagg aaaaaatcct tctcacctgt gtacaggaat
      961 acaacaagaa acttgcggaa aaagctcatt tcatcagtag tatttcgaag atcacaaaga
     1021 aacatgccgt tgtctttacc gataaggatg tcgagaagaa gaacctggag gggaccccga
     1081 ttatcgggaa aaaagaacta cggaaaatcc gcgacccaga ggatgtcttt acgttaattc
     1141 tggagagaat cacctctgat tgacgatgat ctctatgaca caaactttta taaataagat
     1201 tcttattccc gagaactacg ttaccctctg gagtaacaaa tatacttaaa agcgtcgatg
     1261 atgaatcatg gcagacagta aaactgtaga agcagttcaa aaagcaatcg aagaatcaaa
     1321 aaaacttgag cgaaagttca aacaatcggt agacgtcgtc ataaacctca agaacatcga
     1381 catgaacgat gcgaagaacc gtatcgaaga tgaagtgatg ttgcctcatg gtcgggggac
     1441 cgaggcgaaa ctcgctctct tcgccagtgg ggatcttgcc ctgaaatcaa aaaatcacgt
     1501 tgatctgctc attaaacctg aggaaatcga ggaactcgca aaagataaga aaaaattcaa
     1561 aaagatcgca gatgagtatg atttcttcat cgccgaggca ccactaatgc caacgattgg
     1621 aaaaacactt ggtatcatcc ttggtcctcg ggggaaaatg ccaaaaccag taccgccaaa
     1681 catcgatatc gccggtgtcg caaaaagcct tcggaaaacc gtgaaggttc gatcaaagac
     1741 gacaaagacc atccatgcaa ctgttggtcg ggaagagatg aacacggaac atatcgcaga
     1801 gaacattgac gctattttga aacgacttga aggaaaactt gaacgcggga aattaaatat
     1861 cggctcagtg tatgtaaaga caagcatggg gccgccaatg cgaatagtat aaaggtgtta
     1921 gcttatggcg catgtcgcat catggaaaca cggggaggtc acagagatca tttctctgat
     1981 gactcaccat aaaatcatcg gtatcattga tattggaggt attccagctc cacagcttca
     2041 gcagatgcgc ggaggtatcc gggcgaaagc tcagatacgt gcatcaaaga acacgttgat
     2101 tctaaaggcg ttagatgacg ctgaaagtca gataaaaggc atcagtgcct tgaaaaacga
     2161 ggtgcacggc caagctgcaa tcatagcgac tgatatgaac ccgttcgcac tgtttcgaga
     2221 gattaaggcg acccggaaga acgccccagc aaaaggtggc gagatcgctt cccatgatat
     2281 cgaggtcctc gcaggtgata ctcccttcaa accagggccg atcgtcgggg agcttcaaaa
     2341 agcaggaatc ccagcagcta tctcggaagg aaaagtcgtc attaaaagcg ataaagtcct
     2401 cgtcgaagca ggacagaaga tttcacggga acttgctcaa atgctttctc gtcttgatat
     2461 tttccc
//
LOCUS       DATMXJ010000263         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_73105, whole genome shotgun sequence.
ACCESSION   DATMXJ010000263 DATMXJ010000000
VERSION     DATMXJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73105"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..961
                     /locus_tag="VN377_06815"
     CDS             137..961
                     /locus_tag="VN377_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28128.1"
                     /translation="MTPRGKKKLLQAGNILAVLITIIVNALAVILPLNGKTTQELSDA
                     LPNFFVPAGLTFSIWGIIYILWVVFALYQARDMFKKEEIDMPFLSQITWLFILSGVAN
                     STWIFLWHYEYVGLSLLMMILLLGSLLAIYIRLKIGKSTVTMKEKLCVHVPFSVYLGW
                     ISIATIANVTAFLISIQWDGFGIDQLTWTILIIAFGTFLTYLMLALRKDIAFSLVVIW
                     AFFGIWLKRMAQPNTITDMNVATTASIAIVLIVAGLLAVIGYQLMRKNEQKSVQKT"
     gene            1034..1678
                     /locus_tag="VN377_06820"
     CDS             1034..1678
                     /locus_tag="VN377_06820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWR28129.1"
                     /translation="MIKVISFDFDGTIATHAFADAFWLEGVPALYAKQHHIDVETAKK
                     YLFEEYEKIGDNRIEWYDPGYWFDRFNLQTDWKKMLFEYRDYVEIYPEVPSVLKQLSK
                     NYSLIISSNAKKEFIDVQLTQAKLQTYFDKVFSSTSDFHTIKKVTDFYAMICKKMNIS
                     PQEMIHVGDHKEFDFLSPQKLGITSYYLDRKKNTTGPHVVTNLKQFEKRILKRS"
     gene            1877..2821
                     /locus_tag="VN377_06825"
     CDS             1877..2821
                     /locus_tag="VN377_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28130.1"
                     /translation="MKKLLSCLIIGIVVLGGLGAAVTATDQNQKNTQRSGIIESPYVD
                     ELDQSMTDYDGSLPLGRTNIFGYYANLSIAQSFIPQMEVLTRTFFLMARNASTASPCI
                     LAIRENLTEDDLAVISVEPSEFPIVNGTPTEEQLVWIEFNFDDLWVTPGQTYYIVVYT
                     TNITDNYYWISGNGTNIYPNGTVYLSINDGQTWAEFPGGNADGCFKTYGLHETFLQIT
                     LKGGFGPSIIIKNIGNYTAWDVIYDSTISGGLVFIGKHYSNNISELQPGEEITLNLGH
                     VLGFGKVTISLQISAANVKEMSSEHSAILLLFFLLVIK"
     gene            complement(2894..3658)
                     /locus_tag="VN377_06830"
     CDS             complement(2894..3658)
                     /locus_tag="VN377_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HWR28131.1"
                     /translation="MNLVEIKYVEKKPKLKNPIFIEGLPGIGNVGKLAVEHLIDSMKA
                     TKFAELYSKDFPPQVFINPDGTIKLVNNEFYYWKAKKKGQQDIVLLTGDYQGLSSQGQ
                     YELAESILDIVQDLGVKQMFTLGGYGLGREIKEPKVLFATTDVRLVKTMKKYGAIFKK
                     NEPGGGIIGASGLLLGLGKLRGLVGTCFMGETPGYLVDPKSAKAVLKVLTKAVNVHID
                     LSRLEEKAKEIEYIAQQLNEMETMVKEKPDDIRYIG"
     gene            complement(3655..3828)
                     /locus_tag="VN377_06835"
     CDS             complement(3655..3828)
                     /locus_tag="VN377_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HWR28132.1"
                     /translation="MSEILYCNQCQRYTIHPVCRVCKGKTVLNKPARFSPQDHYGKYR
                     RALKKRNREELRQ"
     gene            complement(3828..4616)
                     /locus_tag="VN377_06840"
     CDS             complement(3828..4616)
                     /locus_tag="VN377_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HWR28133.1"
                     /translation="MMKKEYPEEGELVVGTITKVQGFGAFVSLDEYPNKEGFIHISEI
                     ATGWVKRIRNFVREKQKVVCKVVHVDEAKKHIDLSLKKVNDHQRREKIQDWKNAQKAT
                     RLFEMVAQELEKTVDQCYKEFGDALLKKYGTMYAAFEECAYDANTLKNDGFSGDWLKK
                     FEEIAKGSITVPFVEIKGMLLLTSYLPDGITHIKEALNVAEKSEFDDVTIDIKYIGAP
                     RYMIKVKAPDYKIAESQMKKAVERAETYMKKMKGECEFQREVEE"
     gene            complement(4628..4804)
                     /locus_tag="VN377_06845"
     CDS             complement(4628..4804)
                     /locus_tag="VN377_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HWR28134.1"
                     /translation="MDQPKSRFIKVRCNDCENEQVLFDKASTTVLCHICGSKLATPNG
                     GKARIKGTILETIE"
     gene            complement(4809..>5039)
                     /locus_tag="VN377_06850"
     CDS             complement(4809..>5039)
                     /locus_tag="VN377_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HWR28135.1"
                     /translation="TLHEVDKMKKRKASELKRGQRRFRRATSGYGGFPRPKPEGREKT
                     SRRVATRFTCTVCKKMHQPPSIRSKKFEIEGE"
BASE COUNT         1435 a         1154 c          921 g         1529 t
ORIGIN      
        1 gtaccatcaa cgctatttag aaaaacatgg attagccgcc tgtccccgtt aaaaaaaaaa
       61 aaaaatatga atgttttttt tgaagtacgt aaagcttatc tttacgaaac cgattcttca
      121 taagagagga aatagtatga cgccacgagg caaaaaaaaa cttcttcaag caggaaacat
      181 ccttgcagta ttgattacta tcatagtaaa cgcccttgca gtgatacttc cgctcaacgg
      241 taaaaccacc caggaactct ctgatgcgtt accgaatttt ttcgtacctg caggtcttac
      301 gttctccatc tggggcatca tctacatcct ctgggttgtc tttgcgctgt accaagcacg
      361 agacatgttt aaaaaagaag aaattgacat gccgttcctc tcacaaatta cttggttatt
      421 tatcctgagt ggtgtcgcaa acagtacctg gattttcctt tggcactacg aatatgtcgg
      481 tctttcgtta ctcatgatga tcctcttact tggttctctg ctcgcaattt atatccgtct
      541 gaagattggc aaatcaactg tgacgatgaa agaaaaactc tgtgtccatg ttccctttag
      601 cgtctatctt ggctggattt caattgcgac aatcgcaaat gtcaccgcat tcctcatctc
      661 aatacaatgg gatggttttg ggatcgacca attaacctgg acaatcctca tcattgcgtt
      721 cggaacattt cttacgtacc tcatgcttgc cttacgaaaa gatattgctt ttagccttgt
      781 tgtcatctgg gctttctttg ggatctggtt aaaacgaatg gctcaaccaa acacaatcac
      841 cgatatgaat gtcgcgacca cggcaagcat cgccatcgtc cttatcgtcg ctggtcttct
      901 tgcggtcatc ggttatcaac tgatgagaaa aaacgaacaa aaatccgtac agaaaacata
      961 agaataccga tcatttcagt tgtatctccg tagaatacct tttaatacaa cagaggatgt
     1021 atcaacggat tctatgatca aggtaatctc ctttgatttt gatggtacta tcgcgacaca
     1081 tgcctttgca gatgcgttct ggcttgaggg ggtaccagcg ctttacgcaa aacaacacca
     1141 catcgatgta gaaactgcaa agaaatatct cttcgaagag tacgaaaaga taggggataa
     1201 ccgcatcgaa tggtacgacc cagggtactg gtttgaccgt ttcaatcttc aaacagattg
     1261 gaaaaaaatg ctttttgaat atcgagatta cgttgagatc tatccagaag tcccatcagt
     1321 cctgaaacaa ctatcaaaaa actacagcct catcatcagc tccaacgcaa agaaagaatt
     1381 tatagatgtg caactcacac aagccaaatt acagacctac ttcgataagg ttttttcatc
     1441 aacctcggat tttcatacaa taaaaaaagt cacggatttc tatgcgatga tctgtaaaaa
     1501 aatgaacata tcaccacaag aaatgataca tgtcggcgat cataaagagt ttgattttct
     1561 ctccccgcag aagcttggta ttacctcgta ctatcttgat agaaagaaaa acacaactgg
     1621 tccgcatgtt gtcactaact tgaaacaatt tgaaaaaaga attctaaagc gttcataaga
     1681 caatactttc aatcatttac aatagaaata aaacggctgt catattttct atttcccatt
     1741 tcggttcaat gataagtact gtgttaggac aggacatatg tccctacaca gttcaatggt
     1801 aagtatccat aagaaaatcc ttatatacat cattcctata ataattggtt gtaaaataca
     1861 acgggagagg aaaaacatga aaaaattgtt atcatgtctt attatcggca tcgtagtcct
     1921 tggaggacta ggcgctgctg taacagcaac agaccaaaat cagaaaaaca ctcaaagaag
     1981 tggcatcatc gagtcaccgt acgtcgatga actcgatcaa tccatgactg attatgatgg
     2041 atcgttacca ttagggagaa cgaatatttt tggttactac gcaaatctct caatagcaca
     2101 gtcgttcatc ccccagatgg aagtattgac aagaaccttc tttttgatgg caagaaacgc
     2161 atcgaccgca tctccttgca tcttagcaat acgagagaac ttgacagaag atgatctagc
     2221 agttatctct gtcgaaccta gtgaatttcc catagtcaac gggacaccga cggaggaaca
     2281 acttgtatgg atagaattca acttcgatga cctctgggtc accccaggac agacatatta
     2341 catcgttgtc tacaccacta atatcacaga taactattac tggatctctg gcaatggaac
     2401 aaacatctat ccaaatggga cggtctatct ttccattaat gatggtcaga cctgggcaga
     2461 atttccgggg ggaaatgctg atggttgctt caagacctat ggtctccatg aaacattcct
     2521 acagattaca ctgaaaggcg gttttggacc ctcaattatt attaaaaaca tcgggaatta
     2581 caccgcatgg gatgttatat atgattctac aatctctggt ggactcgttt tcattggaaa
     2641 acattactca aataatatat cagaactaca accaggtgaa gaaataaccc tgaatctagg
     2701 gcatgttcta ggatttggaa aagttacaat ctcattacaa atatccgcag caaatgtgaa
     2761 agaaatgtct tccgaacaca gtgcgatcct cctgctgttt ttcctccttg tcatcaaata
     2821 agacatcatt tttctttttc tttttataat ctttttttca ttaaataaga aaagaaaaaa
     2881 attaggagaa ccactaaccg atatatcgaa tgtcatctgg tttttcttta accatggtct
     2941 ccatttcatt aagctgctga gcgatatact ctatctcctt tgctttctcc tcgagccgcg
     3001 aaagatcaat atggacgttc accgccttgg taagcacctt gaggactgct ttcgcactct
     3061 tcgggtcgac aagataccca ggggtctccc ccataaaaca ggtaccgacc aatcctcgaa
     3121 gcttgcctaa accaagcagc agaccactcg caccaatgat accaccacca ggctcgtttt
     3181 tcttaaaaat cgccccatac ttcttcatgg ttttcacaag acgaacatcg gtggtcgcaa
     3241 agagcacctt tggttctttt atctcgcggc caaggccgta cccgccaagg gtaaacatct
     3301 gtttcacacc aagatcctga acaatatcaa ggatggactc tgcaagctca tattgccctt
     3361 gcgatgaaag accctggtaa tcccctgtca aaagaacgat atcctgttgc cctttcttct
     3421 ttgccttcca ataataaaat tcgttattca ccaatttaat agtcccatca gggttaatga
     3481 acacctgtgg aggaaaatcc ttactgtaca actcagcgaa ttttgtcgct ttcatggaat
     3541 caatcagatg ttccacggca agtttcccga cattccctat cccgggtaac ccttcaataa
     3601 aaatcgggtt ctttagtttt ggttttttct ctacatattt tatttctact aggttcattg
     3661 tctcagttct tccctgtttc ttttttttaa cgcccgacgg tatttcccat aatgatcctg
     3721 cggtgaaaac cgagcaggtt tatttaatac tgtttttcct ttgcagacac gacaaactgg
     3781 atggatggtg taccgctgac attgattaca atatagtatt tcagacatta ttcttcgact
     3841 tcacgttgaa actcgcattc acctttcatt tttttcatat atgtttcagc gcgctcaaca
     3901 gcttttttca tctgcgattc tgcaatctta taatcagggg ctttgacttt aatcatatag
     3961 cgaggcgcac caatgtattt aatatcgatc gtaacgtcgt caaactcgct tttttcagca
     4021 acattgagtg cttccttgat gtgcgtaatc ccatcaggga gataggacgt gagaaggagc
     4081 atccctttaa tctcaacgaa aggaaccgtg atgctcccct tggcaatctc ctcgaatttc
     4141 ttcagccaat cccctgaaaa accatcgttt ttcagggtgt tcgcatcata ggcgcattct
     4201 tcaaatgccg catacatcgt cccatatttt tttaagaggg catcgccaaa ttctttataa
     4261 cattgatcaa ctgtcttctc aagctcttgg gcaaccatct caaaaagtct tgttgctttc
     4321 tgcgcattct tccaatcttg gattttctct cgtcgttgat gatcattgac tttttttaag
     4381 gaaagatcga tatgtttctt tgcttcatcg acatggacga ccttgcagac gactttttgt
     4441 ttttctctga caaaatttcg gatacgtttt acccatccgg tagcgatctc ggaaatatgg
     4501 atgaaccctt ctttgttggg gtattcatca agtgaaacaa acgcgccaaa accttggact
     4561 ttggttatcg tacctacaac gagttctcct tcttcagggt actccttttt catcatggag
     4621 aaaaacctta ttcgatggtt tccaatatag ttccttttat ccgagctttc ccaccattcg
     4681 gggtggcaag tttactgccg cagatatggc acagaactgt ggtgctcgct ttatcaaaca
     4741 ggacttgctc gttttcacaa tcattgcatc gtacttttat gaacctgctt tttggttgat
     4801 ccataggttt attcaccttc tatctcaaat ttttttgacc ggatacttgg cggctgatgc
     4861 atttttttac atactgtaca cgtaaatcga gttgcaaccc gtctactggt cttctctcgc
     4921 ccctcaggtt ttggccgagg gaaccctccg tatccgctgg tggctcgtct gaaccgtcgt
     4981 tgtcctcgtt tcagctcact tgccttccgt tttttcattt tgtcaacttc gtgaagcgt
//
LOCUS       DATMXJ010000264         7066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_52119, whole genome shotgun sequence.
ACCESSION   DATMXJ010000264 DATMXJ010000000
VERSION     DATMXJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52119"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VN377_06855"
     CDS             complement(<1..1456)
                     /locus_tag="VN377_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWR28136.1"
                     /translation="MMKKKIICFGTLVLFLCGSVTISGSQSDVSTSFVKDLVRVDITQ
                     GNVVLPEGVEVVAENMGAWVDIIIPRYRLQELTDNQIQYTTLIADMDAYHSAMMGSYH
                     TFVQMENILQSIATNHSDIASLFSIGTSYEGRNLWCLEISDHPGEDEGEPGVLFMGLH
                     HAREWPTVEICLRIANNLTTGYGVNSTITDFVDGRRIWIIPCVNPDGYYYCHDLGNDW
                     RKNRHYFSQYGTYGVDLNRNYPGSADGNAWGAWGSIFDASVTHDPGDEVYCGPGPGSE
                     LEIQAVMNFFTAHDICASISWHTYQQELYWPWGYTASQVPDNTYIGTVGTQIAQRITR
                     QSGSGTYTPGQSHGLYGTTGDNIDWEYGYSHYVMGRTTFAYTIEACSDFHPSASYLDQ
                     ICKENCDGALYLLSEAENIRDTVVPRVMPPLLDELPNDSDGNYRVSWAEQNPLAEPDY
                     FQLDELSGLSIKTDDAESGSGLWSLDGFALSTSRY"
     gene            complement(1475..3367)
                     /locus_tag="VN377_06860"
     CDS             complement(1475..3367)
                     /locus_tag="VN377_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR28137.1"
                     /translation="MKKIIPIFLIGIMVLSGLGAATVSKPSETTLKKTDTIIFSNPEI
                     TTKESDAFISIDNANSWLNTPGAPMLPASVTTYIFPFGTKIKTVDVTFSDPEEYIVEK
                     TIVSAPTPVTYVAGAKVTFDQEESVIGSIYPETLFDYHLGAGISDEDRVLFVTVRCFP
                     IQYKPQDNTILFRDKASLSISYDLPSPETSTADEYSLVIIAPKTFSESLQPLVNHKIS
                     KGITTKLVTRDDICKSVYFPAEGRDCAEEMKYFIKNAYDQWGTDYVLLVGGRYGGILN
                     EKWYVPVRYTNLDDGANWEGGYLSDLYFADIYDAFGNFSTWDSNGNGKFAEWNSQGKD
                     ILDMYPEVYLGRLACKNIDEVETLVSKIITYENTTAGTEWFNKMVVVGGDSAPNATDP
                     WYEGEEENALALEYMTGFDGVRLWTSNGNFTGPQDVSDGINAGCGFLFFDGHGNPMSW
                     STHPPHNESIWINGLQVKDMPKLTNGEKLPVTVCGGCHNGQFNTSLLNILKGFIKEGF
                     KYFQWKFYLGEWTPECWAWKLMSVKNGGSIATMAYTGLDWFATEDWNNDTIPDCTQFY
                     SGYANTQFFKNYGVNNKTILGQAHTQTLIDYLNAFPPMLDKTDCKTVQEFVLLGDPSL
                     QIGGYS"
     gene            complement(3452..5344)
                     /locus_tag="VN377_06865"
     CDS             complement(3452..5344)
                     /locus_tag="VN377_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HWR28138.1"
                     /translation="MNVKIFSILLMTILMCTCFTVIGTQQQNGTITQPEPNSQQPTEE
                     ITIDNDCDCEPIETSATDGLFYRTGLLPAIETLPEGETFTGRTASSWDWRDATYNGQT
                     GDWTTPIRNQANCGSCYAFAPLGSIESLIKIKQGNPGYTIDLSEQFIVSCGTEWMSEY
                     ILGCDGAYSPGPYNFITTYGTLPESCFPYVSGSGSVPPCSDKCSNWEDLIIQIDGWHT
                     VASDITSIKNALIQHGPLTAGMVVYDDFFDYPGGVYEHPGSDPDPMNHAVVIVGYDDA
                     QNCWICKNSWGTSWGEDGWFKILYGDCKIGQEIMYFDYVPQVGPQVNVKIHRIQMIGE
                     IEGWLEGDADWSYRVQVYTGSQWIEQINDAYSSNEDDHTEDVTHRFHVQTPTPEITIK
                     VWDRDTITGDDLADVSGYVGGGTDDSTTDVRGAIFHCQYNLITNQIIQIDTIISEGGY
                     ITSSGTYQPDGGDNSDEENDAKVWFQLSDSYVLPVPNLQVSGSLHGNVNIGTNHYTLG
                     TFVVENIGSDPEGFSDSYLDWAIAETPDWGSNWVFEPNSGQNLPSGDEVAVQVYVDVP
                     DEQGTFSGSVKIWNTENHADYGLISVQLVAPVEKTVLQWDLFCKGFDGTLSKDILSFL
                     LDHCLR"
     gene            5577..6140
                     /locus_tag="VN377_06870"
     CDS             5577..6140
                     /locus_tag="VN377_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HWR28139.1"
                     /translation="MTQQSNRKKELDSLRTEWRKTHRTLNGRIILYPDKAPEHINARG
                     SKLYNWFWGKILRETKPEEIPAIVSGLQDAIHAEKDPRKIQVLRTMIGMADDAYQFKQ
                     LEDIKDTDTELQLENKPVFNSEDGVIYRNGNIYTTDYNEDESEILIRDIEGIIIDVLS
                     TEEKEKNLPPSTVRNNTYKNWNYMEKS"
     gene            complement(6323..6496)
                     /locus_tag="VN377_06875"
     CDS             complement(6323..6496)
                     /locus_tag="VN377_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HWR28140.1"
                     /translation="MYRDNNRGGGYGDAPREMHKVTCAECGKETEVPFKPDGTRPVYC
                     KDCYQKHRPPRRF"
     gene            6787..6978
                     /locus_tag="VN377_06880"
     CDS             6787..6978
                     /locus_tag="VN377_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28141.1"
                     /translation="MAFEKDGYILHTREVQLKGGRNQKIYYFCKTGNKPKSGKPCDMP
                     DGYTTGINKRTKLPYLKKK"
BASE COUNT         1907 a         1589 c         1497 g         2073 t
ORIGIN      
        1 ggtatcgtga ggttgacaga gcaaacccat caaggctcca gagaccagac cccgattccg
       61 catcatcagt ctttatagaa agcccgctta gttcatcaag ctggaaataa tcaggttctg
      121 ctaacgggtt ctgttccgcc catgaaaccc gataattccc gtcagagtca ttgggcaact
      181 catcaagtaa tggtggcatc acacgtggca ccactgtgtc gcgtatgttc tccgcctctg
      241 aaaggagata taatgctcca tcgcagtttt ccttacagat ttgatctaga tagcttgctg
      301 aaggatggaa atccgagcag gcttctatgg tgtatgcgaa ggtcgtcctc cccatgacat
      361 aatgactata tccgtactcc cagtcgatgt tatcaccggt agtgccatag agtccatggg
      421 attgtcccgg ggtataggta ccagatccgc tctgtcgtgt gattcgttgc gcgatctgtg
      481 tgcctacggt acctatgtaa gtattatctg gaacttgtga cgcggtgtac ccccatggcc
      541 aatacagctc ctgttgatag gtatgccagg agatcgatgc gcaaatatca tgagccgtaa
      601 agaaattcat tactgcctgt atttcaagtt ctgaacctgg accaggacca cagtatacct
      661 cgtcccccgg gtcatgagtc actgaagcat cgaagataga tccccatgcg ccccatgcat
      721 ttccatcggc cgacccagga taattccgat ttaaatcaac gccatacgtt ccatactgtg
      781 agaagtaatg cctattcttc ctccaatcgt tcccaagatc atgacagtaa tagtatccat
      841 cagggttcac acaggggata atccatattc tccgaccgtc cacaaaatca gtgatggtcg
      901 aattcacacc ataccctgta gtgagattat tcgcgatacg taaacagatt tccaccgttg
      961 gccattcacg agcgtgatga agccccatga acaacacacc gggttcacct tcatcctcac
     1021 cagggtgatc actgatttct aagcaccaga gattacgacc ctcgtagctt gtcccaatgc
     1081 tgaaaagact agcgatatcc gaatgattgg tagcaatact ctgcaagata ttttccatct
     1141 gaacaaacgt atgatacgac cccatcatcg cggaatgata ggcatccatg tctgcaatga
     1201 gagttgtata ctgaatctga ttgtctgtga gttcttgtaa ccgataccga gggatgatga
     1261 tatcgaccca tgcacccatg ttttccgcga cgacctccac gccctctgga agcactacat
     1321 ttccctgggt tatatcgact cgtacgagat cttttacaaa agacgtagaa acgtcactct
     1381 gtgaaccaga aatggtgaca gatccgcaaa gaaataacac tagcgttcca aaacaaataa
     1441 ttttcttttt catcatttct acattctcct ttacttacga atatcctcct atttgcagac
     1501 tggggtcgcc aagaagaaca aactcctgga cggtcttgca gtcagtctta tcaagcatcg
     1561 gtggaaacgc attgagataa tcaatgagag tctgggtgtg ggcctgacca aggattgtct
     1621 tgttgttcac cccataattt ttaaaaaact gcgtatttgc atatccagaa tagaactgcg
     1681 tgcaatcagg gatggtatca ttgttccagt cttccgtggc aaaccagtca agccccgtat
     1741 atgccatggt tgcgatactc ccaccatttt ttacactcat caatttccat gcccagcatt
     1801 ccggtgtcca ttcgcccagg tagaatttcc attggaagta tttgaatccc tctttgataa
     1861 agcctttcag aatattcaaa agactggtat tgaactgtcc attatggcat ccgccacata
     1921 ccgtgaccgg gagtttttcc ccgtttgtca acttcggcat atccttcacc tgcaacccat
     1981 tgatccatat gctttcgtta tgaggtggat gggtggacca ggacatcgga ttcccatgtc
     2041 catcgaagaa taaaaatccg cagccagcgt tgatcccatc gctcacatct tgaggaccag
     2101 tgaagttccc gttcgatgtc caaagccgaa cgccatcaaa tccagtcatg tactctaacg
     2161 ctaaagcgtt ttcttcttca ccttcgtacc aaggatctgt cgcattcggt gcggaatcac
     2221 caccgacgac aaccattttg ttgaaccatt ctgttcccgc tgtcgtgttt tcataggtga
     2281 ttatcttaga aacaagggtt tcgacctcat cgatgttttt acaggcgagt cttcctaagt
     2341 atacctcagg gtacatgtca aggatatcct ttccttggct gttccattca gcaaacttac
     2401 catttccatt cgaatcccat gttgaaaaat taccaaaggc gtcatagatg tctgcgaaat
     2461 agagatctga cagatagccg ccctcccagt ttgcaccatc gtcaaggtta gtgtaccgga
     2521 ctgggacgta ccatttttcg ttcagaatcc cgccgtatct tccaccaacc aacagcacgt
     2581 aatcagttcc ccattggtca taggcgttct tgatgaaata tttcatttcc tcggcacaat
     2641 ctcgtccttc tgcggggaaa taaacgctct tgcagatatc atcccgtgtg acaagtttcg
     2701 tcgtgattcc tttgctgatt ttatggttca ccaatggttg tagtgattcg gaaaatgttt
     2761 tcggagcaat aatgactaat gagtactcat cggccgtcga tgtttcaggg gatgggagat
     2821 cataggatat tgagagagat gctttgtctc ggaaaaggat tgtattatcc tgtggtttgt
     2881 attggatggg gaaacagcgg actgtcacaa aaagaacacg gtcttcgtcg gagatgcctg
     2941 caccaagatg ataatcaaag agtgtttctg gataaataga tccaattact gattcttctt
     3001 ggtcgaaggt tacctttgcc cctgcgacat aggtcactgg tgtcggtgct gatacgatcg
     3061 ttttttctac gatgtattct tctgggtcgg agaatgtcac atcaacagtt tttatcttgg
     3121 taccaaatgg gaagatgtac gtggtgactg atgcgggtag catcggtgca ccaggtgtat
     3181 tgagccatga gttggcgtta tctatcgaaa taaaagcatc gctttctttg gttgtgattt
     3241 ccgggtttga aaagatgatt gtgtcagttt ttttcagggt tgtttctgag ggttttgaaa
     3301 cagtagcagc tccaagtcca ctgaggacca tgattccaat taagaatatt ggtattattt
     3361 tcttcataat tttatcccct aatttttttt tattaatctc tgatgaagtc tgtccccgta
     3421 tgctcttaat tatataaatc tctatttaaa tctatcttaa gcaatggtcc aggaggaaag
     3481 ataggatatc ctttgataga gttccatcga atcccttgca aaataaatcc cattgcagga
     3541 cggttttttc gacaggtgcc accagttgga cggagataag accgtaatct gcatgatttt
     3601 ccgtattcca tatttttaca gaaccagaaa acgttccttg ttcatcaggt acgtcgacat
     3661 atacctgaac tgcgacctca tctcctgagg gtagattttg gccgctgttt ggttcaaaga
     3721 cccagttgct cccccagtcc ggtgtctctg cgatcgccca atccagatat gagtcgctaa
     3781 atccctccgg gtcactgccg atgttttcca cgacgaacgt cccaagcgtg taatgattgg
     3841 taccaatgtt tacattgccg tgtagcgatc cactgacctg gagatttggc actgggagaa
     3901 cataggaatc tgagagctgg aaccagactt tcgcatcgtt ctcctcgtca ctgttatctc
     3961 ccccgtctgg ctggtatgtc cctgaggatg tgatgtatcc tccttcagag atgatcgtgt
     4021 cgatctgaat gatttggttg gtgatgaggt tatattgaca atggaagatc gctcctcgga
     4081 catccgtcgt gctgtcgtct gttcctccac cgacatatcc actgacatcc gcaagatcat
     4141 ctccggtgat cgtatctcga tcccagactt ttatggttat ctccggtgtt ggagtctgga
     4201 catggaaccg gtgtgtgacg tcctcggtat ggtcgtcttc gttactggag taggcatcgt
     4261 tgatttgttc tatccattga ctcccggtat agacttgcac tcgatacgac cagtccgcat
     4321 ctccttctaa ccatccttcg atttcaccga tcatctggat ccgatgtatt ttgacattca
     4381 cttgaggacc gacctgaggc acataatcaa aatacatgat ctcctgaccg attttacagt
     4441 ctccgtatag gatcttgaac catccgtctt caccccagga cgtcccccag ctgtttttac
     4501 atatccagca gttttgcgca tcatcgtaac cgacgatgac gacagcatgg ttcataggat
     4561 cagggtcgct gcctgggtgt tcgtacacac ctccgggata atcaaagaaa tcatcgtata
     4621 ctaccattcc tgcagtcaac ggaccatgtt gtatcagagc attttttatc gaggtgatat
     4681 cactagcgac cgtatgccat ccatcgatct ggatgatgag atcttcccag ttgctgcatt
     4741 tatctgaaca cggaggaaca gagccagatc cacttacata cgggaaacag gattcgggaa
     4801 gtgttccgta ggttgtgatg aagttgtacg gtcctggaga gtacgcccca tcgcacccta
     4861 agatatattc gctcatccat tcggttccgc aggagacaat gaactgttcg gatagatcaa
     4921 tggtgtatcc tggattccct tgttttattt ttatgagcga ttcgattgat ccgagtggtg
     4981 cgaatgcgta gcagcttcca caattcgctt ggtttctaat tggtgtggtc caatcgccgg
     5041 tctgaccgtt ataggtcgca tcacgccaat cccatgatga cgctgttcgc ccagtgaatg
     5101 tctctccttc cggcagtgtt tcaattgcag gcaacagtcc tgtcctgtaa aagagtccat
     5161 ctgtcgccga tgtttcaata ggttcacagt cgcaatcatt gtcaatagtg atttcttcgg
     5221 ttggctgctg tgaatttggc tcaggttgtg taatagtacc gttctgttgc tgtgtcccga
     5281 tgacggtaaa acaggtacac atcaatatgg tcattaacag gattgaaaaa atttttacgt
     5341 tcatgatttt tgttccctcg atagtcagca gtatctatca actataaata tttttatcta
     5401 ttatagaaaa atttaatcat attaaaaaag attgaatatt ttttttacct cggccaacgc
     5461 atttcagcaa gaaccgatgg ttcaaccttt gtgattttat cacgtaatac tctttttctc
     5521 atacaataca atcctccata atgtttatat cctaaaacgt ataataataa cgtgaaatga
     5581 cacagcagag caaccggaaa aaagaacttg attcgctgag aaccgaatgg cggaaaacac
     5641 atcgaactct caatggcaga attatcttgt acccggataa ggcgcctgaa catatcaatg
     5701 cgagaggcag taagttgtat aactggttct gggggaagat actgcgtgag accaagccag
     5761 aggagatccc tgctattgtg tctggtctgc aggatgcgat ccatgcggag aaagatccaa
     5821 ggaagataca agtactgaga accatgatcg ggatggctga tgatgcgtat caattcaagc
     5881 agcttgagga catcaaggat actgatacag agttacagtt agagaacaaa ccggttttta
     5941 actctgaaga cggagtcatc tatcgaaatg ggaacatcta tacgactgat tataatgagg
     6001 atgaaagcga gatccttata cgggatatcg aagggattat tattgatgtg ttgtccactg
     6061 aagaaaaaga aaaaaacctt cctccatcga cagttcggaa taatacgtat aagaactgga
     6121 attatatgga gaaaagctga ttcgccaaca tataatacaa tagaggagtt tcttaggaat
     6181 attttattga agtttttttg taaatgctct atatatttat gttttaacga ggaatatata
     6241 aaaaaaacct gacattcagg agtaactcat gttttgtgcg aatcgattac agatatgaaa
     6301 agaaaaaaaa agagagagag atttaaaatc tccttggtgg ccgatgtttc tgataacagt
     6361 ccttacagta aactggtcta gtaccatctg gtttaaaggg aacttctgtt tcttttccac
     6421 attctgcaca tgttacctta tgcatttctc gtggtgcgtc accatagcca ccgcctctat
     6481 tattgtctcg atacattttt cattacatcc ttttttttgg ttgcgtaaat aatacaatca
     6541 ctatataata ctatacattg acggatagag ttcagtgatc tgagcgcagg gtaagggatt
     6601 tgaacaccaa tttaccggca tgataataat tgacgatggt gttggttgtc attttaatgg
     6661 tgctacgatt aatgtctcta atctttatta ttatactaga tttttttgtg cagatggtcg
     6721 ctgagatccc tttggccagg gaacctttat atgatactcc tccttacact actccgagga
     6781 aataatatgg catttgaaaa agacgggtat atcctacaca cacgtgaagt ccagctgaaa
     6841 ggcggaagaa accagaaaat atactatttt tgcaaaacag gaaacaaacc aaaaagtggc
     6901 aaaccctgcg atatgcctga tgggtatacc actggtatca acaaacgaac taaattacca
     6961 tatctgaaaa agaaataaat ttttcttttc tccttttttt agaaaacaga ttctgtttta
     7021 aaaaaatatc cttaaaacct ctatttatta atcggttttc aattac
//
LOCUS       DATMXJ010000265         6769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_73143, whole genome shotgun sequence.
ACCESSION   DATMXJ010000265 DATMXJ010000000
VERSION     DATMXJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73143"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..2449
                     /locus_tag="VN377_06885"
     CDS             59..2449
                     /locus_tag="VN377_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWR28142.1"
                     /translation="MKSTIYLVIGILVLSGFTTLSMGDADQKQGNLRFSFSEPTIVEK
                     ESYVQLNSEGTDSWIFDAGSPMLPRRIETVTLPFGATIQNIVCVATGVESKMLSQKIM
                     PAPQPMPLSSEVTFTTGPEMNPEIYNSEVLFPADWMSYHVGAGLDENNQHKTFLTINT
                     YPVRYKPGSDTIYYASDIDVTVTYTVPDKTPFPANTEYQLVIIAPSKFSSALQPLIDH
                     KISKGISTTLKTTEDIYAEYTGYDKPEQIKYFIKDAIETWNTTYVLLVGGLKSRVYAV
                     PRDSASEGSKGWYLPVRYSSLKTSAEDDPGYISDLYYADIYNATGDFCSWDSSGNHIY
                     AEFGGINADKLDLYPDVALGRLSCRSTKEVKGVVDKIITYETTADPSWFNKMVLVSGD
                     GFLDQVDLGISWNTLSLPNGAYTIYAQSKNINNVYGPIDTLHITIDKTKATTLTFNHD
                     DHLITGLKYPFPPVAEIVSVSEGDVLGNTDYSYSPTEREAYLNSQLHWADLQYTSGVL
                     RIRGKTYDPRPYGVETSIHVWVNNSAGTTVFTQTRNGFKMYWEGEWTCGDHLLLGRAG
                     GAYYMPSEFEKTYLFSSNGQWTGQTEVLNAISQGAGFVFFSGHGSPNIWANHYPGVPG
                     NRANGDLVGLAAVQIKLPIFPMDTLTNEYKNPVVVVGGCHNSMFNVSLIPTFLDMKNQ
                     HSTHTYGTPTAECWSERIVNLPKIGAIASMGNTGYGYGILNEFCTTGGLDNYITTEFF
                     VQYGTDGHHVLGQAYAGTLTEYISHFKAEGWDGAHQKTVEQWVLLGDPSLLIGGYS"
     gene            complement(2560..2802)
                     /locus_tag="VN377_06890"
     CDS             complement(2560..2802)
                     /locus_tag="VN377_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28143.1"
                     /translation="MKPRKADYVFLIAYLIILIFLLFITPVTVIEGTGSSQEINTPLL
                     PEIAWLLLYIIGTPLLYLLLRRFIYPRKKQTNHPNL"
     gene            complement(2910..3152)
                     /locus_tag="VN377_06895"
     CDS             complement(2910..3152)
                     /locus_tag="VN377_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28144.1"
                     /translation="MEFDPVSLFLWVILPYLAIVTIYFGIMIWSLRKVKKRLAQIESR
                     LVQKIEKESMEKEKNREQKLMRMKNEIDDMLHDGNR"
     gene            complement(3385..3588)
                     /locus_tag="VN377_06900"
     CDS             complement(3385..3588)
                     /locus_tag="VN377_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28145.1"
                     /translation="MTAIPPLLKYFIDNGTTIKLFNEIVSKYCVSEEKHAQCVNFKLF
                     EQGMRPPVELLPNGKKSNSLTSF"
     gene            complement(3635..3763)
                     /locus_tag="VN377_06905"
     CDS             complement(3635..3763)
                     /locus_tag="VN377_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28146.1"
                     /translation="MGCPYYEDFTRLCITRFASIIKYSSSQTYESEKYTNCLVYNV"
     gene            3924..5195
                     /locus_tag="VN377_06910"
     CDS             3924..5195
                     /locus_tag="VN377_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWR28147.1"
                     /translation="MLKHVTLDGVRLHLKPDERKLWINSKYVLFLNETTCFILEALIE
                     SCYEVAKEQVPERTVRKIVRKYRVSKEKAREDLDAIVGVINGFAREEPPLHLIGMTVI
                     DPGSMKAPGRMDLSLTYQCNNRCPQCYLKENADVMGSLETAQWKTVIDRLWEVGVPQV
                     VFTGGECLLRDDLVELVEYSKQFTTGIITNGTLLSPELANKLRDAHLDWIQITLEAAS
                     AKIHDEIQGRNGAWEETVAGIKNAVAAGLSVSVNATLSKKNAKELKKLIAFARKLNVR
                     YVSTNAIINAGRGIAEKEESGLEEEKLTRILDEAKAYAQSHDVVFNWFLPTCYKNVNP
                     VELGFGQRCCSACSVNMMVEPNGDVIPCQSWTQQKLGNILSDPWERIWDHPVAQQIRN
                     HAFVEEECKGCEHLEVCMGACPLDHLNQGGI"
     gene            5196..6632
                     /locus_tag="VN377_06915"
     CDS             5196..6632
                     /locus_tag="VN377_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HWR28148.1"
                     /translation="MMKAGRMFSGCLFLGVVMFICCAMSSVKADTGTYTISSQNVDLW
                     VQTDGDVIIHYEIGMLVHTGNIPWVTVGLPTSNFDISSSGGDATSVIPQNSAGWSGVY
                     VTLDKTYYANERFLFSFTVLQKEFIYKYNDQQASLLFTPCWWDNAVVEQMNVTIHLPP
                     EIHSVTTTSQPTLFMNSSVVWSWDTIPAGEKKSTGMLMPLSVFSHVGPGPGLSDFLTR
                     YSWVPLFAGALIAVVIVLVLILRRGSRSAYEDPQMYTGGSRKLIKHFNLDCPNDGGRL
                     DRRSFHGTTIDFCETCGGSYFDKGEVEALLEAGVNEQEFNTKQVASFRDYSAPVGTCP
                     RCDGMMETVTRSSEHKDYHIYVCKDCRGIWMNKNIFQAIKEKRLEQDEQQQKKLVATG
                     DKKTELKKAYYAPNWWWFYPYIFYPRQYQKDLTPVVPVQHSCACVSCACVSSCACACA
                     CAGGGAAGCAPKDMLYPQIMFRVRQRKR"
BASE COUNT         1737 a         1384 c         1769 g         1879 t
ORIGIN      
        1 ataataaata cctatcaata tctactataa gaaaaatatt agggggtttt aaaaaaatat
       61 gaaaagtaca atctatttag tcataggaat ccttgtacta agtggtttta caacactaag
      121 tatgggggat gctgatcaga aacaagggaa ccttcggttc tccttttcag aaccaactat
      181 tgtagaaaaa gagtcttatg ttcaattgaa ctctgaaggg acagactcct ggatttttga
      241 tgcaggcagt ccgatgcttc ctcggcgtat tgagaccgtg accttaccat ttggtgcgac
      301 gatacagaac atcgtctgcg tcgcaaccgg ggtcgagtcc aagatgttgt ctcagaagat
      361 tatgcctgct ccacaaccca tgccgttgag cagtgaggtg acttttacca ctgggccaga
      421 gatgaaccct gagatctata actcggaagt cctcttccca gctgattgga tgagttatca
      481 tgtgggggct gggctggatg agaacaatca gcataagacg ttcttgacaa taaacacgta
      541 tcctgttcgg tacaaacccg ggtctgatac gatttattac gcaagtgata tcgacgtgac
      601 cgtcacctac acagtacctg acaagacgcc gtttccagca aacaccgagt atcaattagt
      661 catcatcgca ccgtcgaagt tctccagtgc tctgcagccg ctcattgacc ataagatctc
      721 caaagggatc tcaacgacct tgaagaccac ggaggatatc tacgcggaat acaccgggta
      781 cgataaacct gagcagataa aatatttcat taaggatgcg attgagacct ggaataccac
      841 ctatgtcctg cttgtcggtg gtttaaaatc tcgcgtgtat gcagtcccac gagacagcgc
      901 cagtgaagga tcgaagggtt ggtatctacc ggtgagatac tcaagtttga agacaagcgc
      961 ggaggatgac ccaggttata tctctgatct gtattatgct gatatttaca atgcgaccgg
     1021 tgatttctgc agctgggatt caagtgggaa tcatatctat gctgagtttg gtggcatcaa
     1081 cgcagataaa cttgatctgt accctgatgt tgcgttagga aggctttcct gcagaagcac
     1141 aaaggaagtc aagggtgtcg tagataaaat tatcacctac gaaacaactg cggatccatc
     1201 ttggttcaat aagatggtgc tggtgtcagg cgacgggttc ctggatcaag tagatctggg
     1261 tatctcctgg aacaccttaa gtctgcccaa tggggcgtac accatttatg cgcagagtaa
     1321 gaatattaac aatgtctatg gacccattga tacactccac atcaccattg ataagacaaa
     1381 ggcaacaacg ctgaccttta accatgatga ccatctgatc accggtttga aatatccgtt
     1441 cccaccggtc gctgagatcg tgtccgtctc cgaaggggac gtgcttggta acacggatta
     1501 ttcgtattct ccgactgaac gtgaggcgta cctcaacagt cagttgcact gggcagacct
     1561 ccaatatacg agtggggttc tgaggatccg tgggaagacc tatgatccac gaccgtatgg
     1621 tgttgagacc agtattcatg tctgggtcaa caatagcgcc ggaaccacag tatttaccca
     1681 gacgagaaat ggttttaaga tgtattggga aggggaatgg acctgcggag atcatctgtt
     1741 actcggacgc gcaggaggag catattatat gccatcagag tttgagaaga catacctctt
     1801 cagctccaat ggacagtgga ctgggcagac agaagtgtta aacgcgatca gccagggggc
     1861 tgggttcgtg ttcttctccg gtcatggcag tccaaatata tgggcgaacc actacccagg
     1921 tgtgcctgga aatcgggcta atggtgattt agtaggatta gctgcggtac aaatcaaact
     1981 gccgatcttc ccgatggata ccctcacgaa tgagtacaag aaccctgtgg tggtcgtcgg
     2041 cggctgtcat aacagcatgt tcaacgtctc actcattccg acgttccttg atatgaagaa
     2101 tcagcattcg acgcatacct acgggactcc gaccgctgag tgttggagtg aacggatcgt
     2161 caacctccct aagatagggg caatcgcgtc gatgggaaac acggggtacg ggtatgggat
     2221 cctcaatgag ttctgtacta ccggtggtct ggataactac atcaccactg agttcttcgt
     2281 ccagtatggg accgatgggc atcatgtcct tggacaggcc tatgcaggta ctctgaccga
     2341 gtacatcagt catttcaagg cagaaggatg ggacggggca caccagaaga ccgtggagca
     2401 atgggtgctg cttggtgacc caagcctgct gatcggtggg tactcataat cgagtactct
     2461 ttctttctct ttttttttct ttttaaataa aaaaggttta ccattctttt ccagggttta
     2521 actcttaggt ttatatatca taggtggtat gtctcttctt tagaggttag ggtgattggt
     2581 ctgttttttc cgcgggtaga taaacctacg aagtaaaagg taaagtagtg gtgttcctat
     2641 gatatagaga agtagccatg cgatttcagg taaaagcggt gtatttatct cttggcttga
     2701 gccagttccc tcaatgacag taacaggtgt gatgaataac aggaagatca atattatgag
     2761 gtacgcgata agaaacacat agtctgcttt tcttggtttc atctgtttcc tccgttctgt
     2821 ttatgcatct tttttgatgt ataaatgctt tttaggggcc ctattttgtt gataaataaa
     2881 aataacgtat ttattaaata agaggtagat tatcgatttc catcgtggag catgtcgtcg
     2941 atttcatttt tcatccgcat gagtttctgt tccctgtttt tttctttctc catgctttct
     3001 ttttcaattt tttgcaccag tcttgattct atttgtgcca gccttttttt gaccttacga
     3061 agggaccaaa tcataatacc gaaatagatg gttactatcg ccaaataggg cagtattacc
     3121 cacagaaaaa gagatacggg gtcaaattcc attcatccac ctcatcaggg acgatattgt
     3181 catgtcacct attaacattt ttggatgccg ttcaaaatct gagagagttt tacgaattta
     3241 ttgatgagaa aaattagaga gtattttgta agttattaag ttttaatata tcactggtgg
     3301 tatgatattt tcgaagagat atgagaagag gattacgatc ttttgttact gtttctacgg
     3361 tttaatcaat ggtgatttcc tttttcaaaa agatgtcagt gagtttgact ttttaccgtt
     3421 tggcaggagt tcaaccgggg gtcgcatccc ttgttcaaac agtttgaaat ttacgcattg
     3481 ggcgtgtttt tcctcagaga cacaatattt tgaaacgatt tcattgaaga gttttatggt
     3541 ggtaccatta tcgataaaat attttaacaa tggagggatg gctgtcaccg cttcttcagc
     3601 acagtgttct atctatttac agttgaattt ttttttacac gttgtatacg aggcagttgg
     3661 tgtatttctc cgattcatag gtttgagaac tcgagtattt tattatggag gcaaatcgtg
     3721 tgatgcagag tctggtgaaa tcttcgtagt acggacaacc catgctatct catctttatt
     3781 ttttgggcat ttaatttctt tttctctttg aatacatgcg tatttccaag agtgggaaga
     3841 cggtgataat cttgaggttt atctatagat aaatatcgaa agttttaaag gatagttatc
     3901 tgataaaatg ggaggtttgt cttatgttga aacatgtgac gttggatggt gttcgccttc
     3961 atctgaaacc tgatgagcgg aagctgtgga tcaatagtaa atatgtgttg tttctgaatg
     4021 agacgacatg ttttatcctt gaggcattga tcgagagttg ttatgaggta gccaaagaac
     4081 aggttcctga gagaacggta aggaagatcg ttcggaagta tcgggtttca aaggagaagg
     4141 cgcgggagga tctcgatgcc atcgttggcg tcatcaatgg gttcgcccgg gaggagcctc
     4201 cgctgcatct gatcgggatg accgtgattg acccggggag catgaaggct ccaggccgga
     4261 tggatctttc gttgacatac caatgcaata atcgttgtcc gcagtgctat ctgaaagaaa
     4321 atgctgatgt gatgggatcg ctggagactg cgcagtggaa gacggtgatc gatagattat
     4381 gggaggttgg ggtcccccag gtggtgttca ccggcgggga gtgtctgctt cgggatgatc
     4441 tggtggagct tgtcgagtat agtaagcagt tcaccaccgg gatcattacg aacgggacgt
     4501 tgctttcccc ggaacttgcg aataagcttc gtgatgcgca tcttgattgg atccagatca
     4561 cgctagaggc tgcttccgca aagattcatg atgagatcca gggaaggaac ggtgcatggg
     4621 aagagactgt tgcggggatc aagaatgcgg ttgctgccgg attgtcggta agtgtgaatg
     4681 cgacgctttc aaaaaagaat gcaaaggaac tgaaaaaact cattgctttt gcacggaaac
     4741 tgaatgtccg gtacgtgtcg acaaacgcga tcatcaacgc aggacgtggg attgcagaga
     4801 aagaggaatc tgggttggag gaagagaagc ttacgaggat tctggatgag gcgaaagcgt
     4861 acgcacagtc ccacgatgtc gtgtttaact ggttcctgcc aacatgttat aagaatgtga
     4921 atccggtgga gcttgggttt gggcagcgat gttgttctgc atgttcggtg aatatgatgg
     4981 tcgagccgaa tggtgatgtg atcccctgtc agagctggac gcagcagaaa cttggtaata
     5041 tcctgtctga tccctgggag cggatctggg atcacccggt tgctcagcag atcaggaatc
     5101 atgcgtttgt agaggaggag tgcaagggct gtgagcatct tgaggtatgc atgggcgcct
     5161 gtccgttgga tcatctgaac cagggaggta tttgaatgat gaaagcagga cggatgtttt
     5221 cagggtgttt gttcctcggt gttgtcatgt tcatctgctg tgccatgtcc agtgtgaagg
     5281 ctgatactgg gacgtatacg atttcttctc agaacgtgga cctatgggtt cagaccgatg
     5341 gggacgtcat catccactat gagatcggga tgctggtgca caccgggaat atcccatggg
     5401 tgaccgtcgg gttgccgacg tcaaatttcg acatcagttc gtccggtggg gatgctacat
     5461 cggtgatccc tcagaacagt gcaggttggt caggtgtcta cgtaactctt gataaaacct
     5521 actatgcgaa cgaacggttc ttgttctcat ttacggtact tcaaaaagag ttcatctaca
     5581 agtataacga tcagcaggca tccttgttgt ttaccccctg ctggtgggac aatgcggtgg
     5641 tggaacagat gaatgtcacg atccatctcc cacctgagat ccacagtgtg acgaccacgt
     5701 ctcagccaac cttatttatg aactcgagtg tcgtctggtc ctgggatact atccctgctg
     5761 gtgagaagaa atccacaggg atgctcatgc cgctctcggt gttttcacat gtgggaccag
     5821 gaccgggatt gtcagatttt ctcaccaggt atagttgggt gccgttgttc gcaggtgcgt
     5881 tgatcgctgt tgttatcgtg ctggttctga tcctgcgccg tggatcccgg tccgcgtacg
     5941 aggacccgca gatgtatacc ggtggctctc gaaaactgat taagcatttc aacctggatt
     6001 gcccgaatga tggtggcagg cttgatcggc ggtcgtttca tgggaccaca atcgatttct
     6061 gcgagacctg tggcggaagc tattttgata aaggcgaggt ggaagcgtta ctcgaggctg
     6121 gagtcaatga gcaggagttc aacaccaaac aggttgcttc gttccgcgac tattccgcac
     6181 cggtgggaac atgcccgcgg tgcgatggga tgatggagac ggtgacccgg agcagtgagc
     6241 ataaagatta tcatatctac gtctgcaagg actgccgagg catctggatg aacaaaaaca
     6301 tctttcaggc gatcaaggag aaacgcttgg aacaggatga acagcagcag aagaaattag
     6361 tcgccactgg tgataagaag accgaactga agaaggcgta ctatgcaccg aactggtggt
     6421 ggttctaccc gtacatcttc tatccgcgtc agtatcagaa ggacctaact ccggttgtac
     6481 ctgtgcagca ttcgtgtgcg tgtgtctcct gcgcctgtgt gtcaagttgt gcgtgtgcct
     6541 gtgcatgcgc tggcggtggt gctgcggggt gcgctcccaa agacatgcta tatccgcaga
     6601 tcatgtttcg agtaaggcaa agaaaaaggt gagaaagaga ttacttttta ataatggaaa
     6661 catgattcgc gagggatggt tgtaaaattt gaaaaacgcc atgaatccct tcttccgcgc
     6721 tctaaatttt tcctccgtct cgtgaagttc gccctcctct ctataggac
//
LOCUS       DATMXJ010000266         6146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30473, whole genome shotgun sequence.
ACCESSION   DATMXJ010000266 DATMXJ010000000
VERSION     DATMXJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30473"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VN377_06920"
     CDS             <1..439
                     /locus_tag="VN377_06920"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWR28149.1"
                     /translation="GLFDAIDGKVAKLTNKASARGDFLDHVLDRYADVFMVGALALSS
                     WCRPPIGLLAIVGVLLTSYMGTQAQAIGHRRDYSGLLGRADRLVLLIIFPIVQHFVLR
                     SSLQLPWDFTILELVLVYFAVVGNITAIQRFFVTLRWFRGGRR"
     gene            complement(683..820)
                     /locus_tag="VN377_06925"
     CDS             complement(683..820)
                     /locus_tag="VN377_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28150.1"
                     /translation="MMTAPTRLPYFARGYTCNDCQFKGIPLIFSSEKIYKKFLLLLKR
                     T"
     gene            1027..2307
                     /gene="hflX"
                     /locus_tag="VN377_06930"
     CDS             1027..2307
                     /gene="hflX"
                     /locus_tag="VN377_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWR28151.1"
                     /translation="MNTTPKKAILISLDDSTSEITQLAETLGYTILKTFIQHRTNPDV
                     NYYIGIGKVEEITDYTKDTEDQIDLIIVNGELKPSQWFALEKKLETKVYDRIRLILAI
                     FEEHAERQEARLQVKLAQLQYERPYVRELIHRTKAGEHPGLMAGGEYQVDDYYEMIKR
                     QTKKIREDLQKLRENRELQRQTRSQSGFYSVSLAGYTNAGKSSLMNLLSGEKVKVEEQ
                     LFSTLSTTTRSISTKNKEKQIPILLTDTVGFIENLPSCIIDAFHSTLEEIELADVVVL
                     VVDGSEAKEVVEKKLRVSINELRNIGVGSPIIIALNKIDLIETPTLNTLRDYLKNLDI
                     IETHIVVTISVKEQKNIEELLQTIYDSLPHMVKITFQLPLEEHSQRFISQLYEKTKVL
                     QITYGSLITVEVECNEKIKEKLIASCRAVNGQIL"
     gene            2362..3399
                     /locus_tag="VN377_06935"
     CDS             2362..3399
                     /locus_tag="VN377_06935"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HWR28152.1"
                     /translation="MMNLKNQGNLQQLITIIGLILLGTIGRYVLFGMGVQPFPNFEVI
                     MVVTFLAVMLIRSPLALIVPLISMIGSDLLIGNPIFVGDQMNRIVLFTYSGFAIIGLI
                     TLLNKNRLWRNLGQFRLKSLGLVAGLGIGSVLLYDIWTNMGWWYLMYPHDTSSLIMVY
                     TAGLPFMVYHLISGIVTFCAIGLPVILYAAKKKDQMHLHPFQLKMIHKIPAVLLVLSL
                     VALSFTGTALKVPQKSEVWVEKSDQTSVRIIIRGDSWTLTDNLVAYQGDTAFSLLERS
                     GQQNGFSVDATYYAQFDSTLVNSINNAVGGTDGKYWQYYVNGKLPEIGADKCILVNGD
                     VLTWSFEVPPS"
     gene            complement(3428..4138)
                     /locus_tag="VN377_06940"
     CDS             complement(3428..4138)
                     /locus_tag="VN377_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HWR28153.1"
                     /translation="MTSILTWILLIFLSFLPPMIYTIWIRNTERCHREQWLSIFLCFI
                     WGATVAVVAALILEILLELPLQYTLSSGDTTPFLLAVVIAPIVEEFTKPLALYLRTVK
                     TNLDELEDGLIFGAVAGLGFSATENLLYGYSFLSEGFLLFLLVIATRSIGGCLLHASA
                     TALTGYGIGKTMKKHSSFIGILPYFLLAIFLHGFYNFLLSYEFIGVLSGLALAFLFVI
                     ITITLIRKKIRTLDEKNC"
     gene            complement(4384..5589)
                     /locus_tag="VN377_06945"
     CDS             complement(4384..5589)
                     /locus_tag="VN377_06945"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR28154.1"
                     /translation="MIRKCLAFGIILLFVGTCITPGIAQNTEKQSSRGNWLYVGGSGP
                     GNYSIIQDAINDSSDGDTVFVYDKSSPYYEHLKITHQVNLIGENRETTIIDGNGNMNI
                     SVVLLADDGRNVTISGFTIRNGLRGIKVNTNNNIISGNIIDNTSQGICLSGSDSNTVA
                     GNIITHTMFGVYISCSFQNKIYENYIEDNLLGIEVYCDWSREYVKNTLFFVEGFPNDI
                     YKNTIRNNSYGIYVLGESLVNISHNDIINNSNGINLEISSSAGSLWCNNNTIFQNNIS
                     RNEHGITLYSRGYMRVTGGTGGWGIRDNNIVENNITYNANGIEITNYMRHGIGFNTFS
                     HNNFIGNTQTVNLENTIYNHWVGNYWETPRLLPYPIIGEIMIFRHIIHWINFDWHPAQ
                     ELYNIPGMS"
     gene            complement(5640..5984)
                     /locus_tag="VN377_06950"
     CDS             complement(5640..5984)
                     /locus_tag="VN377_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28155.1"
                     /translation="MDKKPLIVVSICAVVLLVLGSLSNVVGYQTSHVSNNVEIDVTLN
                     SEINQLSDPECDCEKNEPPTRPILCGLLSPLHSILDVLSHISIISAFFNDMLNVIGLF
                     LGCSWAPGVPPE"
BASE COUNT         1907 a         1346 c         1161 g         1732 t
ORIGIN      
        1 cgggttgttt gacgcgatcg atgggaaagt tgcgaaactt accaacaaag ccagtgcgcg
       61 tggtgatttc cttgaccatg ttctcgatcg gtacgcggat gtcttcatgg ttggtgctct
      121 ggcattgagt tcttggtgtc gtcctccgat tggtttgtta gcgatcgttg gtgtgttgtt
      181 gacgagttat atggggacgc aagcccaggc aattggtcat agacgtgatt attctgggtt
      241 gttgggtcga gctgaccggt tggttctgct gattattttt ccgatcgttc aacatttcgt
      301 gcttcgttct tcgcttcagt tgccctggga ttttaccatc cttgagttgg ttttggttta
      361 ttttgcggtt gttgggaata tcactgcgat tcaacgtttc tttgttacct tgcggtggtt
      421 tcgtggaggg cggagataag aggattgtgt agttttttgg tatttgcatg gaagatgtat
      481 ggctataatg aattaaataa gggagggaag ggaaaacatt aaggggattt aatgaagaat
      541 aagacccttc ctccgcttct cagataaaag aagagttaca tccatatata aaattttcta
      601 cgttttttca taacatttgt aacaagattg agtcttcttg cctccttttt aggtttgcta
      661 gaaaaaaggt tttttttttg attcatgttc ttttgagcag aagtaagaat tttttataga
      721 ttttttctga ggaaaaaatt aagggtattc ctttgaactg gcagtcgtta caggtgtagc
      781 ctcgtgcaaa gtacggcagt cgtgttggtg ccgtcatcat cgaatgagtg tttttgcttc
      841 cacacaatgg acatgcgatt atctccataa tctcatcaat gatatgttat agcatagcac
      901 atatataaat cttatgtcaa aaaaagacaa ttttatagag ggaacatata aaaaccaagc
      961 tctgccccgg acatcagcgt tgccaaaaat taaagtagaa cagctcgtta ttcaccccag
     1021 aaaatcatga acaccacccc aaaaaaagca atcctcatat cactcgacga ctcgacatca
     1081 gagatcaccc agctcgcaga gaccctcgga tataccatcc ttaaaacatt tatccaacac
     1141 cgaacaaatc ctgatgtcaa ctactatatc gggatcggaa aagtagagga aataaccgat
     1201 tacaccaaag acaccgaaga tcaaatcgat ctcatcatcg taaacggcga actcaaacca
     1261 tcacaatggt ttgccctgga aaaaaaatta gagaccaagg tctacgaccg catccgactc
     1321 atcctagcca tcttcgaaga acacgcggaa cgacaagaag cacgacttca agtaaaactc
     1381 gcacagctac aatatgaacg accctatgtt cgagaattaa tccaccgaac aaaagcagga
     1441 gaacaccctg ggcttatggc cggaggagaa taccaggtcg acgactacta tgaaatgata
     1501 aaacgacaga caaaaaaaat aagagaagac cttcaaaaac tcagggaaaa cagagaacta
     1561 caacgacaga ccagatccca gagcggtttt tactcagtct ccctcgctgg ttacacaaac
     1621 gcaggaaaaa gcagcttaat gaacctacta tcaggagaaa aagtaaaagt cgaagagcaa
     1681 ctcttttcca ctctttcgac aacgacacga agtatttcga caaaaaacaa agaaaaacaa
     1741 atcccaatac tgctgaccga tacggtcgga ttcatcgaaa acctcccatc atgcatcatc
     1801 gatgcattcc attcgacact tgaggaaatc gaacttgctg acgtcgtcgt cctagtcgta
     1861 gacggaagcg aagcaaaaga agtcgtggaa aaaaagctcc gtgtgtcgat aaacgaatta
     1921 cgcaatatcg gtgttggctc acccatcatt atcgccttaa acaaaatcga tttaatcgaa
     1981 acaccaacac tcaacaccct cagagactac cttaagaacc tagatatcat cgagacacac
     2041 atcgtcgtta ccatttcagt gaaagaacag aaaaacatcg aagaactgct gcagaccatc
     2101 tatgattcgt taccacatat ggtgaaaatc acgtttcaac tccccttaga agaacactca
     2161 caacgattca tctctcaact ctatgaaaaa accaaggtgc ttcaaatcac ctatggctct
     2221 ctgatcacgg tcgaagtcga atgcaacgaa aaaatcaaag aaaaactcat cgcttcctgt
     2281 cgagcggtga acggtcagat tctgtaagaa aacaaaactc ttatacgctg aattgtattt
     2341 cctacaataa gggagtatgt tatgatgaat ttgaaaaatc aaggaaatct acagcagctg
     2401 ataacaatca tagggttaat ccttttaggg acgatcggac gatacgtcct cttcgggatg
     2461 ggagtccaac cattcccgaa ctttgaagtc atcatggtgg tgacgttcct tgccgtcatg
     2521 ctcatccgat caccactcgc actcatcgtc cccttgataa gcatgatcgg aagcgacctg
     2581 ctcatcggaa atccaatttt tgtcggagac caaatgaaca gaatcgtgtt attcacctac
     2641 tcaggtttcg caatcatcgg cctcatcacc ctgctcaaca aaaatcgact ctggagaaat
     2701 ctcggacagt tccgcctgaa aagccttggc cttgtcgcgg gacttgggat cggatcggtc
     2761 ttgctctatg acatctggac aaacatggga tggtggtacc ttatgtatcc tcatgatacc
     2821 tcctctctca tcatggtgta tactgcaggc ttaccattca tggtgtacca tctgatctcc
     2881 ggaatcgtca cgttctgtgc gatcggactt cctgtcattc tctatgctgc gaagaaaaaa
     2941 gaccagatgc atctccaccc gttccaacta aaaatgatcc acaagatccc cgcggtcctt
     3001 ctcgtcctta gcctcgtcgc actatcattt actgggactg ccctaaaggt cccacagaaa
     3061 agcgaggtct gggttgagaa atcagatcag acaagcgttc ggatcatcat ccgtggcgat
     3121 agctggactc ttacagacaa cctcgtcgcc taccagggag acacagcatt ttcgttattg
     3181 gagcgaagcg gacaacaaaa tggattttct gtcgacgcaa cctattacgc gcagttcgac
     3241 tcgacactgg tcaattccat caacaatgca gtcggaggaa cagacggaaa atactggcag
     3301 tattacgtca acgggaaact gcctgaaatc ggcgctgaca aatgcatcct cgtaaacggg
     3361 gacgtcctta catggagttt tgaagtccca cccagttaaa taaaaaacat ctctcctttc
     3421 ttttttttta acagtttttt tcatcaagcg tacgtatctt cttccgtata agggttatcg
     3481 tgataatgac aaaaagaaac gcaagggcaa gtcctgacag aaccccgatg aattcatagg
     3541 agagaagaaa gttatagaac ccatgaagaa agattgcgag gagaaaatat ggtagaatac
     3601 caataaacga agaatgtttc ttcattgttt ttccgatccc gtatcccgtc aatgcagtcg
     3661 cggatgcatg aagcagacag cctccgatgc tgcgcgtcgc aatgaccagt aaaaacagga
     3721 ggaatccttc agagagaaaa ctgtatccat acaggagatt ctctgtagca gaaaacccta
     3781 atcctgccac cgctccgaag atcaaaccat cctctaattc atcgagattt gtcttgactg
     3841 ttctgaggta caacgcaaga ggtttggtga actcctcgac gattggtgca atgacgaccg
     3901 ccaggagaaa tggtgtcgtg tctcctgaag ataacgtgta ctgtaatggt aattctaaaa
     3961 ggatttccaa aatcaacgca gcgacaacag cgacggttgc accccagatg aaacaaagaa
     4021 aaatcgataa ccattgttcc cgatggcatc gttcagtgtt ccggatccat atcgtgtaaa
     4081 tcataggagg aagaaatgag aggaagataa gaagaatcca tgtcagaatc gacgtcatta
     4141 ccagaagaaa acaaatatgt ctttattatg ttttttaaaa ttctccgaac taagttttat
     4201 gtcttccaac atttctacac gtcctcataa ttcaaatcgg ttgcaagttc ttctgttttt
     4261 agctgcattc ttatagtaga attcttctac ttttgcgtgt aaaaagaaaa cgcttcttcg
     4321 tttgctggat taactctggt aactattcct caagcatcat cgacaatttc agatggtctc
     4381 ttttcaactc atccctggga tgttatagag ttcttgtgct ggatgccaat cgaagttaat
     4441 ccagtgaata atatgccgaa atatcattat ttccccaata atcggatatg gaagcaatct
     4501 cggggtttcc cagtaattcc caacccagtg attatagatt gtattttcca aattcacggt
     4561 ttgagtattt ccaatgaaat tgttatgaga gaaggtattg aagccgattc catgccgcat
     4621 ataattagtt atctctatcc cgtttgcatt ataggtaata ttattttcaa cgatgttgtt
     4681 atccctgata ccccaaccac ctgtaccacc agttactctc atgtaacctc tactgtacag
     4741 agttatccca tgctcatttc tactgatgtt attttgaaat atggtgttat tattgcacca
     4801 caaactaccc gcactgctgc taatttctaa atttatgcca tttgaattat tgataatgtc
     4861 gttgtgggaa atattgacca aactttcacc taaaacgtat attccgtagc tattattcct
     4921 gatagtattc ttataaatat catttggaaa accttcaacg aagaacaaag tattttttac
     4981 atactctcga ctccaatcgc aatacacctc gatccctagt aaattatctt ctatataatt
     5041 ctcgtaaatc ttattctgaa aagaacaaga aatatagacc ccaaacatag tgtgggtaat
     5101 gatattacca gcaaccgtgt tactatccga accagataaa catatccctt gtgaggtatt
     5161 atcgataata ttgccagaaa tgatattatt attggtatta acctttatcc ctcttaatcc
     5221 atttctaatg gtgaatccac tgatagtaac atttctcccg tcatccgcca ataacacaac
     5281 agatatattc atattcccat tcccatcaat aattgtggtt tctctatttt ccccaatgag
     5341 attgacttga tgagtgattt ttaaatgctc gtaatatggt gacgatttgt cgtaaacaaa
     5401 gacagtatcc ccatcagacg aatcattaat cgcatcctga attattgaat aatttcctgg
     5461 tccacttccg ccaacatata accaattccc tcttgatgat tgtttttcag tattttgtgc
     5521 aatccctggt gtgatacagg ttccaacgaa caagagaatg attccaaacg ccaaacattt
     5581 ccttatcatt tttctcccat atcatataat tacgatattg cattattttg atttccaaat
     5641 tattctggag ggacaccagg tgcccaagaa caacctagga acaaaccaat tacatttaac
     5701 atatcattaa agaaagcaga tattatagag atatgtgata aaacgtcaag aatggagtgt
     5761 aacggagata aaagaccaca caatattggt cgagttggtg gttcattctt ttcacaatca
     5821 cactctggat cggataattg atttatttct gaattgagtg taacatctat ttcaacatta
     5881 ttagatacat gagatgtttg atacccaacc acgttactca acgaacccag aacaagaaga
     5941 accacagcac agatacttac cactatcaga ggtttcttat ccatcttgct ttcccccatt
     6001 atcatatttt ataacaatgt tctcttataa gaagattatt aatagaggtt ccgtcagata
     6061 aaacagtatc atttcaggat atcacataaa attgtagaat ccttatcatt ttttatctta
     6121 tgacaaaata ttgaccactc taaata
//
LOCUS       DATMXJ010000267         8431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30600, whole genome shotgun sequence.
ACCESSION   DATMXJ010000267 DATMXJ010000000
VERSION     DATMXJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30600"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..2079)
                     /locus_tag="VN377_06955"
     CDS             complement(97..2079)
                     /locus_tag="VN377_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HWR28156.1"
                     /translation="MVVLRQVKKISVLVICGLFLGAGLLPIMNGAQTTENKTHEITMD
                     IATRVATAKLQELNKRDISITEKTQITNEEGEPLFYVFNLNPPGYLIITGSYDLPPVI
                     AYSFTSNFQANAKDGPLFDLLYADLTLRLKYIQYIPGRTLEERHLQWDTYSKGIPLSN
                     GRFEQWPPEGSTPTGGWLLTNWHQNAPYNNFCPLDLANGGARSAAGCPAVTIAQILNY
                     HNTTMNVVFNDSDDYYHNYGGNQFWIDNNYVTYGFKSFPQLNTYLFTLQSHYESQTPP
                     TNDDKAAITFACGVAATQVYGASGSGTFGVNQAYEAYQRFNCTTISLLDEDDPDLYNR
                     LSSNMKDALPAHLAVVNEGWTVGHNLVVDGYNTDNYFHVNFGWGGSYNGWYLIPEELP
                     YDLTVIEGVIVDILKNTAVPDLSCEGTLEWNNVTPNETVTSSFTVSNIGEDGSNLDWN
                     ITEWPSWGEWAFTPSSGNNQQPDDGPTAVTVNVVVPDQENHLFTGEVKIENLENSSDY
                     STISVSLQTGYKIHEKLFCNGSLTWSDVKPKSTITGSFTIENVGAAFTNLSWEVTEWP
                     DWGTWTLTPSEGDHLTPEDGPVTINVSVVAPLKRNIEFSGQIKIVNSENSSDFDTIPV
                     TLATPYQFHLTILDILQTLLERYPHAFPLLRLLINS"
     gene            complement(2156..5101)
                     /locus_tag="VN377_06960"
     CDS             complement(2156..5101)
                     /locus_tag="VN377_06960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWR28157.1"
                     /translation="MPKQMSEKTFSNLLDYYIACLEQEDLLSVTFNVSSENILFLSTL
                     FQTEVLFHTNNKQVEIKNTEGAKKFFQTSLLRQKNKTLFYGYPVVIGPQGTISPLFFT
                     ELHYEQKEDILIITHDTNEYKLNHHILSKQNFSAEEIKNIRHEIEGDTFFTALESICK
                     MLQFPYTECTTILDSKPFKRTIVQKLLNKAILYFGERSDITYNLITELMQLKKKPLDE
                     LASSALLLLLTGDYQPEKTNQDDKPFLEIFSLNPAQKNAIQKSLHNPLTVITGPPGTG
                     KSQVVLNIIANAVYHNKTVLFASKNNKAVDVVIEKLNAILPYKLLVRMGHQAHRRNAR
                     AGLEHLIKQPIQKIPLQEKTFSELWTVIDQIECIQDQIFSLTSLNESLEKTQTALDFV
                     QEQHPNALTVQEQHSHLDMIDPLILQDDLRKYFSTHSILKQLNPKRYQRKQDQCFRKH
                     YERLPPGLQIHLQNTITNNQTTKEAALQWILTWKKEELILDEIQRIKKTLFTIPPFAE
                     LKNKQATLQKKYITLSQTLLQHHWINKFVDAQENDARHIQTYFSSSEQLESWGGDEVV
                     FRQLHNQRVQALQKILRFLPVWVVTNLSAKQSFPLKNNIFNLLIIDEASQCDIASALP
                     LFYRANHIVIIGDPYQLKHISLLTETQDRSLATTHHISEELYTNVSYTKHSLYDFAEQ
                     IIHQHSEQPLLLNEHYRCHPDIISFSNEYYYGRKLTIATDETKLLHHSSFHKRILWHH
                     VKGKTIHTKSPYNEEEAERVVEEILKILEIVSSAHASIGVVTLFRAQTEMITEKLKKF
                     QDIFETDITIGTAHRFQGDEKDIIVFSPAVSEGIKPGTLHWIQTTSQLLNVAVTRARS
                     LFIIIGDQEICRHTTGPLRNLYDYVESISAQSFTTNSPTKQIFYNEAKQNGIPIIPNY
                     LIKGNTPYQLDFAFFVNGNRYAIEIQSKQTKINRQQLLVDGWKIRRFSEQNIRHNLSE
                     TIEELKRLC"
     gene            complement(5244..7052)
                     /locus_tag="VN377_06965"
     CDS             complement(5244..7052)
                     /locus_tag="VN377_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWR28158.1"
                     /translation="MEPILQVHNLSKSIAGKQLFENTSFEIYPEDCIGLLGPNGCGKT
                     TLFNILLGNEQPNSGDVQKKDGLKIRSLEQDPMHRSDTTLNDYFTRTAQTENIQLQIK
                     NLEQQLEDPAIYESNRHQEILDKLRALQTQTTKTSGGAQLEAARERLKELGLTGITLD
                     MQMKNFSGGERQKIAIASILTQPQNCDLFLFDEPTNHLDIETIEWLEQKIADIPSAVV
                     IISHDRYLLSDLVDRVFEFHGSHLELYDASYEEYEEQKEIREHIKKQEYQRAAIEMKR
                     QLAAIKKITRRNRYNIQINSRMKQIEKLKHVENPILKNYLLRFHFKTVLKSGKNIADG
                     VGLSKYFGEKRILSNANFEIFAGQKIGLLGPNGCGKTTFLKMLTKEEPYSQGKLTLSQ
                     GAKWGYFDQGHLSLNLENTLLDEILRGHIDLSEEDAKALLGQFNFKGTTIHNKVGQLS
                     GGERARLAFLRLIMEPYNLLLLDEPTNHMDIESKTAIETALNTFTGTVIVVSHDRKFL
                     DTVTDTIFFMTDADIKIYSGNYSMFRQQRLKELTEYTDKDLAYLSSGLTKYVVTKGFT
                     IWTIKKKHTVGEEILIGDHNRNIYDWAIKGGLLKEA"
     gene            complement(7107..>8431)
                     /locus_tag="VN377_06970"
     CDS             complement(7107..>8431)
                     /locus_tag="VN377_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWR28159.1"
                     /translation="LILRRRYLYLRLGMRNIRIDTYFFGALLGPILIVLFGILNLPQI
                     LTGLGGVGSLNPFGILVLFLSMVFMSIFLDITGFFEYCARVALKYASGNGRRLFFSLY
                     LTVSILTIFTSNDIIILTFTPFIYYFSKNAGIDPKPYLIAEFFAANTWSMMLYIGNPT
                     NIVLAAAFNLRFDEYTKWMLLPSLAAGFLNMIILYVIFKKKINQPLKQTETIKPRSAI
                     TDPTSTILGLSMLSGCIVCLAIAPYIGVEMWIISLVFGLALLLLLLLRDTLVAITKEH
                     IEKKHFTVKTTIKKMPLSIIPFILSLFITVEALRIYGITKDIGIFFDNLSGASTTATT
                     FIYGISSAFTANILNNIPMTVAYVPIIQTATHTNILPAVLATTIGSNLGANITPIGAL
                     AGIMWMSILRDKEVKLTFKEFVTYGLLVTPVTLLACLGILAAEFLFFL"
BASE COUNT         2074 a         1579 c         2029 g         2749 t
ORIGIN      
        1 ccatcggaag ccccggcatt tatgccgggg aggatgtcac atattcttgt tgactgtaac
       61 gatgaatggt gatcttcgtt tgtacatttg aatgagttag ctgttgatga gaaggcgcag
      121 caacgggaaa gcgtgcgggt atcgttccaa aagcgtctga agaatatcta aaatagtcag
      181 atggaactgg tatggtgttg caagggtcac agggattgtg tcgaaatcac tgctattttc
      241 gctgttgact atctttatct gaccgctgaa ctcgatgttt cttttgagag gtgcgacgac
      301 tgacacatta atggtgaccg gtccatcctc gggtgtcaga tgatctccct ctgacggagt
      361 caaagtccag gtgccccagt ccggccactc ggtgacctcc cagctcagat tggtgaatgc
      421 tgcaccaaca ttctcgatgg tgaaacttcc agtgatcgtc gactttggtt tgacatcgga
      481 ccaggtaaga gaaccattgc agaataattt ttcatggatt ttgtaccctg tttgtaatga
      541 gacggatatg gtggaataat cactactgtt ttctaggttt tcgattttta cttcccccgt
      601 gaaaagatga ttctcttggt caggtacgac gacattgacg gttacagcgg ttggtccgtc
      661 atcaggttgt tgattatttc cacttgaggg tgtaaaggcc cattctcccc agcttggcca
      721 ttcggtgatg ttccaatcaa ggtttgagcc atcttcaccg atgttgctga ccgtgaagct
      781 gctcgtgacc gtttcatttg gtgtcacatt gttccactct agagtcccct cacaactaag
      841 atcaggtaca gccgtgttct tgaggatgtc aacgatgact ccttcgatca ccgttaaatc
      901 atacggaagc tcctcgggga taagatacca tccgttatag gatccgcccc atccgaagtt
      961 cacatggaag taattgtccg tgttgtatcc atcgacgacg aggttatggc caaccgtcca
     1021 tccctcgttt actaccgcaa gatgagctgg gagcgcatcc ttcatgttgc tagataatcg
     1081 attatacaaa tctggatcat cctcgtcaag taaggaaatg gtcgtgcaat tgaacctctg
     1141 atacgcctcg tacgcctggt tcacaccaaa cgtccctgag ccactcgctc cgtagacttg
     1201 ggtcgcagcg accccgcagg caaaggtgat tgctgccttg tcatcgtttg tcggaggtgt
     1261 ctgactctcg taatgtgatt gaagtgtaaa aagataggtg tttagttgag gaaatgattt
     1321 gaaaccatat gttacataat tattatcaat ccaaaactgg ttccccccat agttgtgata
     1381 atagtcatca ctatcattaa aaacaacatt catcgttgta ttatgatagt ttaatatctg
     1441 ggctatcgtc accgcaggac atccagctgc acttcgtgcg ccaccattag caagatcaag
     1501 tggacaaaaa ttattgtagg gagcattctg gtgccaattg gtcaacagcc atcctccggt
     1561 cggggtcgat ccctctggag gccattgttc aaatctccca ttcgaaagag gaattccttt
     1621 ggaatatgta tcccattgaa ggtgacgctc ttcaagtgtt cttccaggta tatattgaat
     1681 atattttaga cggagcgtta gatcagcata cagcaaatcg aaaagaggac catcctttgc
     1741 gttcgcttgg aaattgctgg taaatgagta cgcgatgacg gggggaagat cgtacgatcc
     1801 tgtaatgatg aggtaccctg gagggtttag attgaacaca tagaataatg gttctccttc
     1861 ttcattggtt atttgtgttt tttccgttat cgagatatct cttttgttaa gttcttgtaa
     1921 ttttgcggta gcgactcttg tagcgatatc catggttatt tcatgggttt tattctcagt
     1981 cgtttgagcg ccgttcatga tcgggagaag gcctgctcca agaaacaatc cacagataac
     2041 aagaactgat atttttttta cttgacgtaa cacaaccatg atttttcccc tttattacta
     2101 attgtatatc ggtcatggta tataaatatt gattgaaaat tttatggttc ttagattagc
     2161 ataagcgttt gagttcttca atcgtctcag aaaggttgtg tctgatattt tgttctgaga
     2221 accgtctgat tttccatccg tctaccagta attgctgcct gtttattttg gtttgtttac
     2281 tttgtatttc gatggcatat cgatttccgt tcacgaagaa cgcgaaatca agttgatatg
     2341 gggtatttcc ttttatcaaa taattgggta taatcggaat gccattttgt tttgcctcat
     2401 tgtaaaaaat ctgctttgtt ggggagttcg ttgtaaacga ttgtgcgctt atgctttcta
     2461 cgtaatcata gagatttcga aggggacctg ttgtgtgccg acagatttct tgatcgccaa
     2521 tgataatgaa taaacttcgt gctcgtgtga cagcaacgtt taacaattga cttgtcgtct
     2581 gtatccagtg gagggtacct ggttttattc cttcagaaac cgctggtgaa aaaacaatga
     2641 tatctttttc gtctccttgg aatcgatgag ctgtgccgat ggtgatgtct gtttcaaaga
     2701 tatcttggaa ttttttgagt ttttcggtta tcatctcggt ctgtgctcga aaaagagtaa
     2761 caacccctat cgaagcatgt gcggaagaaa caatctcaag gatttttagt atttcttcca
     2821 cgaccctttc tgcttcttct tcattataag ggcttttcgt gtgaatggtt tttcctttga
     2881 catgatgcca aaggatcctt ttgtggaacg aggaatggtg cagtagtttt gtttcatcgg
     2941 ttgctatggt aagttttcgt ccataataat actcgttaga aaacgaaatg atgtctggat
     3001 ggcatcggta atgttcattc aataataagg gttgttcact gtgttggtgg atgatctgtt
     3061 cggcgaaatc ataaagagaa tgttttgtgt acgaaacatt ggtgtagagt tcttcgctga
     3121 tatgatgtgt tgttgcaagt gaccgatctt gggtttctgt gagcaatgat atgtgtttga
     3181 gttgataggg gtctccaata atgacgatgt gatttgcgcg gtagaaaagt ggcaatgctg
     3241 aggcgatatc acattgggat gcttcatcga ttatcaagag attaaagata ttatttttaa
     3301 gggggaagct ttgtttcgcg gagagattgg taacgaccca cactggtaag aatctaagga
     3361 ttttctgcaa tgcctgtact cgttgattat gaagctgccg aaacacaacc tcatctcctc
     3421 cccacgattc aagttgttca gatgatgaaa aatacgtctg aatatgtcgt gcgtcattct
     3481 cttgtgcgtc tacgaatttg tttatccaat ggtgctgtaa taatgtttgt gagagcgtga
     3541 tgtatttctt ctgcaacgtc gcttgtttgt ttttcaattc agcgaacgga ggtatagtga
     3601 ataatgtttt ttttatacgt tgaatctcat cgaggattaa ttcttccttt ttccatgtca
     3661 gaatccattg taatgcggct tctttcgttg tttgattatt cgttatcgta ttctgtagat
     3721 gaatctggag gccgggtgga agacgttcgt aatgctttct aaagcattga tcttgctttc
     3781 gttggtatcg tttcgggttc aattgtttta aaatactatg agtactaaaa tattttcgaa
     3841 ggtcatcttg gagaatcaac gggtcgatca tatcgagatg cgaatgttgt tcttgaacgg
     3901 tcagggcatt tggatgttgt tcctggacga aatcaagagc cgtctgtgtt ttctccaacg
     3961 attcgttgag gctggtgagg ctaaaaattt gatcttgaat acattcaatt tgatcgatga
     4021 ccgtccataa ttcgctgaag gttttttctt gtaatggaat cttctgaatg ggttgtttta
     4081 tgagatgttc gagtcctgct ctcgcgtttc ttctatgcgc ttgatgcccc atccggacga
     4141 ggagtttgta gggtaatatc gcgtttaatt tttcgatgac gacatcaact gctttgttgt
     4201 ttttactggc aaagagcacg gttttattgt gataaacggc gttagcgatg atgttgagca
     4261 caacctggga ttttccggtc cctggtggtc cggtgatgac cgttaacgga ttatgcaagg
     4321 atttttgtat tgcatttttt tgagcgggat tgagtgagaa gatctctaaa aagggtttat
     4381 catcttggtt cgttttttca ggttgatagt cgccggtaag taaaagcagc agtgcggaag
     4441 acgctagttc gtcgagtggt tttttcttta actgcatgag ttctgtgatc agattgtagg
     4501 tgatatcgga tcgttctccg aagtacagga tcgctttatt gaggagtttt tgaacgatcg
     4561 tacgtttgaa gggtttcgag tcgagaatgg ttgtacattc ggtgtatgga aactgaagca
     4621 ttttacagat tgattcaagt gcagtaaaaa aggtgtcccc ttcgatttca tgtcgaatgt
     4681 ttttgatttc ttctgccgaa aaattttgtt ttgaaaggat atgatgattg agtttgtact
     4741 cgtttgtgtc gtgagtgatg attagtatat cttctttttg ttcatagtgg agttcggtga
     4801 aaaacagagg gcttatggtt ccttgaggcc caatcacgac tgggtaccca tagaacagtg
     4861 ttttattttt ctgccgaagc aaggaggtct gaaaaaactt tttagcacct tctgtgttct
     4921 tgatttccac ctgcttgttg ttggtgtgaa acaacacttc cgtttggaag agggtggaca
     4981 agaacaagat attttccgag gagacgttga aggtgacgga cagcaggtct tcttgttcga
     5041 ggcaagcgat gtagtagtca agaagattgc tgaaggtctt ttcagacatt tgcttcggca
     5101 ttgtcctccc agacaactgc gttcatcggg gtcttttgtt cccctttgaa cgaagggtat
     5161 aatacgtcaa tcctatataa atcttttttt tgtattataa tatttagata tttctatatt
     5221 tatgaaaaac aaatttctca attttacgct tcttttagaa gtccgccttt gattgcccaa
     5281 tcatatatgt tgcggttgtg gtccccgatc agaatctcct caccaactgt atgcttcttt
     5341 ttaatggtcc agatggtgaa tcctttcgtt acaacatatt tggtaagtcc tgaggagaga
     5401 tacgcaaggt ccttgtcggt gtactcagta agctctttga gtcgttgttg acgaaacata
     5461 gaataattac ctgaatagat tttaatatct gcatcggtca tgaaaaaaat cgtgtcagtt
     5521 acggtatcaa ggaactttcg atcatgggag acgacgataa cggtccctgt aaacgtgttg
     5581 agggcagttt cgatcgctgt ttttgattca atatccatat ggtttgtcgg ctcgtctaac
     5641 agcaggagat tgtacggctc catgattaat cggaggaaag caagacgagc acgttcccct
     5701 ccggagagtt gaccgacttt attatggatt gtcgttcctt tgaaattgaa ctggccaagg
     5761 agcgcttttg cgtcttcctc cgataaatcg atatggcctc tgaggatttc gtctagaaga
     5821 gtgttctcta aatttagtga caggtgtcct tggtcgaagt acccccattt tgcaccttga
     5881 ctcagcgtca gttttccttg gctatagggt tcctcttttg tcagcatttt taggaaggtc
     5941 gttttcccgc atccgtttgg accgagaagt ccgatttttt gcccagcaaa aatctcaaag
     6001 ttcgcgtttg agaggatcct tttctccccg aaatactttg aaagaccaac accatctgcg
     6061 atgtttttcc ctgatttcaa aacggtttta aaatggaatc tgaggagata atttttcagg
     6121 attggatttt ccacatgttt gagtttctcg atttgtttca tgcgactgtt gatttgtata
     6181 ttatagcggt ttcttctggt gattttttta atcgcggcta gctggcgttt catttcaatg
     6241 gccgctctct gatattcctg cttcttgatg tgctccctga tctctttttg ttcttcgtat
     6301 tcttcatagg atgcatcata cagttcaagg tgtgaaccat gaaattcaaa gaccctgtcg
     6361 actaaatcac ttaaaaggta gcggtcatga gatatgatta cgacagcgct tgggatgtca
     6421 gcgattttct gctcaagcca ttcaatcgtt tcaatgtcca ggtggttggt cggttcatca
     6481 aaaaggaaca ggtcgcagtt ttgtggttgt gtgaggattg aggcgatcgc gattttctgc
     6541 cgttctcctc cagagaagtt tttcatttgc atgtctaagg tgattccagt aagacccagt
     6601 tctttgagac gttctcgtgc tgcttcgagt tgggcgcccc cgcttgtctt ggtcgtctgc
     6661 gtctgtaacg ctctgagctt atcaagaatt tcttgatgac gatttgattc atagatggca
     6721 ggatcttcca attgttgttc taagtttttg atttgtagtt ggatgttctc tgtttgtgca
     6781 gtccttgtaa agtagtcatt taaggtggtg tcagatcgat gcatcggatc ttgttcgaga
     6841 gagcggattt tcagtccatc ttttttttgt acatcaccac tatttggttg ttcgtttcca
     6901 aggagtatgt tgaacagggt tgtttttcca cacccgtttg gcccgaggag tccgatgcag
     6961 tcctccgggt aaatctcaaa cgatgtgttt tcaaaaagtt gttttccagc gatggatttg
     7021 ctcaggttat ggacttgtag tattggttcc atggtcggca gggtaatctt cttctgtggt
     7081 atatggtttt aggttcgttt tcctttttat aaaaagaaca gaaactccgc agcaagaatc
     7141 cctaagcagg caagcaaggt gactggagtt acaagcagcc cgtaggtgac aaattctttg
     7201 aaggtgagtt tgacttcttt atcacgtaag atgctcatcc acatgatgcc ggcgagtgca
     7261 ccgatcggtg tgatgtttgc cccgaggtta gaaccaatag tggttgcaag gactgctgga
     7321 aggatgtttg tgtgggtggc agtctggatg atagggacat atgctaccgt cattgggatg
     7381 ttgttcagga tatttgctgt aaatgcactg gagattccat agatgaaggt cgtcgcggtc
     7441 gtcgacgctc cactgaggtt gtcaaaaaaa ataccgatgt cttttgtgat tccgtatatc
     7501 ctgagcgctt ctacagtgat gaacaaggaa aggatgaagg ggatgatgct cagcggcatt
     7561 ttttttatgg ttgtcttgac cgtgaaatgt tttttttcta tatgttcttt tgtgatggcg
     7621 accagggtat ctcgcaggag gaggagtagt aagagtgcaa gtccgaatac taaagaaatg
     7681 atccacattt ctacgccgat gtagggggcg attgcaagac aaacaatgca gccagaaagc
     7741 atggagagtc cgaggattgt gcttgtcgga tctgtgatcg ctgatcgtgg ttttattgtc
     7801 tctgtctgct tgagaggttg atttattttt ttcttgaaaa taacataaag aatgatcatg
     7861 tttaaaaaac cggctgctaa cgaagggagt agcatccatt tggtgtactc atcgaatcgg
     7921 agattaaacg ctgcagcaag cacgatgttg gtcggatttc cgatatacag catcatactc
     7981 caggtgttag ctgcgaaaaa ttctgcgatg aggtagggtt tggggtcgat tcctgcgttt
     8041 tttgaaaagt aataaatgaa tggtgtgaat gttaggataa tgatgtcgtt agaggtgaaa
     8101 atggttagga tggagaccgt cagatataaa gagaaaaaga gacgtcgtcc gtttccactc
     8161 gcatatttca aggcaactcg cgcgcagtat tcgaagaatc cagtgatgtc taaaaagatg
     8221 ctcataaaga ccatggagag aaacaaaact aaaattccaa aagggttcag gcttcctaca
     8281 ccaccaaggc ctgtgaggat ctgggggagg ttgaggatgc cgaacagcac aatcaggatt
     8341 gggccaagca gcgcaccaaa gaaatacgta tcgatccgaa tgttgcgcat cccgagtcgc
     8401 aggtagagat accgtctcct aaggataagg t
//
LOCUS       DATMXJ010000268         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_52284, whole genome shotgun sequence.
ACCESSION   DATMXJ010000268 DATMXJ010000000
VERSION     DATMXJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52284"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VN377_06975"
     CDS             <1..248
                     /locus_tag="VN377_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWR28160.1"
                     /translation="PNAINRFHAAFFLGIKKEKRAVNALIDALQDGDGNVVANVIYAL
                     GEIGDPQALEPLRKLNDAGVEKLRRIAIEKIRQIKGS"
     gene            complement(387..2015)
                     /locus_tag="VN377_06980"
     CDS             complement(387..2015)
                     /locus_tag="VN377_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWR28161.1"
                     /translation="MMKSKIICLVVCMLMIVTVFPVVGLSIQTGETKNFNITQPRIQP
                     PGPAYVPTPSLSTITDAPIIREHPEKTTATMDDLVISIIQQVDESIFLSYEENLTANG
                     PRPTESPSCIAAAEYMYDQFQSFGLAVRYHHWSNGGYISDNVEATLNGTDETSDEIYI
                     ICAHYDTVSAGPGADDDTSGTAAVLIAASIMSQYQFNHTIKFVAFSGEEQGMLGSEVY
                     ASEAADQGWNIIGVLNCDMISYAATTNDGNNLIVFEDTASDWLYTYTVAINNEYNEYI
                     QLTLEYGGDTWGSDHWYFWQNGYSALFYFEYNETPYYHTAQDTMAHINATYAVKNMRL
                     IFATLAELAHGEPLSNPPATPMLTGPDSGLTNEIYTFNAVTTDPDGDYIYYLFNWGDG
                     NNSGWLGPFTSGTPANGSYIWNTPGSYDITVKAKDENGIESGMSPAHTIIINPAIQIE
                     TITGGLFKVKVVLKNNGASTATNVPWWINLSGGILLLGKTTKGTIPEIAAGDSVTISS
                     KMIVGFGKTVITVTADTATTSQSATVLLFFIKTP"
     gene            complement(2169..2897)
                     /locus_tag="VN377_06985"
     CDS             complement(2169..2897)
                     /locus_tag="VN377_06985"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HWR28162.1"
                     /translation="MRSRYVRKPALRRVVTLLDVTKCRVQRLLRHVWTDLSAWSLVLT
                     NMVTISAAFLEQWSFSTIIWVYWCQILIIGIFAFLRTIALTDLSLTGVERIRLRFTLK
                     GKLRTGTIFLAHFTVVQLCLTFVLWLVIGPVTNVNGTILLFTSLLFFTNHLFSYIYNK
                     KRERVVHKNLGFAILRPYLRMLPLFVAFLFAGIIIASLYLSRDAQLEPVGYLVLFVLK
                     TSADVIAHSLEHSPPTRWKIRKFF"
     gene            complement(3111..3362)
                     /locus_tag="VN377_06990"
     CDS             complement(3111..3362)
                     /locus_tag="VN377_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28163.1"
                     /translation="MTFTKENQKERNRFIDQWADYVRTHSDQDWSRQQNVIINSCLRT
                     ASMTKEQYLAMKHIEGEMKQGKNVKKLTQKEAVKYLKNL"
     gene            complement(3349..3924)
                     /locus_tag="VN377_06995"
     CDS             complement(3349..3924)
                     /locus_tag="VN377_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28164.1"
                     /translation="MRFIDTTTLEINREPSDLDRLALDFTTVLEKHTLYVIVSGYVSI
                     LLGRARASEDIDIIIPKIPSPTLKKLVHDLKEKGFYSINEEKGSDIYACLTEGRTVRF
                     AKQKIMIPNIELKSAKNRIQGLALQKTITVILPQGTLHISNLELQIAFKEKVLRSPKD
                     REDARHIQKVAEGHLDKKLLKHYKEMLRDVH"
BASE COUNT         1042 a          808 c         1009 g         1297 t
ORIGIN      
        1 gtcctaatgc tattaataga tttcatgctg catttttttt agggatcaag aaagaaaaga
       61 gagcggtaaa cgcattgata gatgctctgc aagacggaga tggtaatgtc gttgcaaatg
      121 taatctatgc tcttggcgaa attggcgatc ctcaagcact agaacctctg cggaaactga
      181 atgatgctgg cgttgaaaaa ctacggagaa tcgcgattga aaaaatcaga caaatcaaag
      241 gatcataata gagatattca attaattaac atagatgaac tggatatttt aatcctgcaa
      301 tgaagagtct ttggctagga atcaaatgag aaagatacga agggggagtt gtttcccctc
      361 taggtttctc ttgctgctat tgtatgctac ggtgttttga tgaagaataa gagcactgtt
      421 gcactctgtg atgttgtagc tgtgtctgca gtgacggtta tgactgtttt gccgaaacca
      481 acgatcattt tcgaggaaat tgttactgag tcaccggcag cgatttcagg aatggttcct
      541 ttcgttgttt ttccaagtag gaggatgcct cctgagagat tgatccacca ggggacgttc
      601 gttgctgtag atgctccgtt gttctttagg acaactttca ctttaaataa tccaccagtg
      661 attgtttcaa tctggattgc gggattaatt atgatggtgt gtgctggtga cattccactc
      721 tcgataccat tttcgtcttt tgctttcact gtaatatcgt aagacccggg agtgttccaa
      781 atatacgaac cattcgcggg agtgccggag gtaaacggtc caagccatcc tgaattgttg
      841 ccatcgcccc agttgaacag atagtaaata taatctccgt cggggtcggt ggtgacagcg
      901 ttgaaggtgt atatttcatt ggtgagcccg gaatctggac cggtgagcat tggtgtcgcc
      961 ggaggattgc tgagtggttc tccatgagcg agttctgcca gagttgcgaa tataagtcgc
     1021 atgtttttta ctgcataggt cgcgttgatg tgtgccattg tgtcttgagc ggtatggtaa
     1081 tagggagtct cgttgtattc aaagtagaac aatgcggaat acccattttg ccagaaatac
     1141 caatgatcgc tcccccaggt atctccgcca tactctagtg tgagttgaat atattcgtta
     1201 tattcattgt tgatggctac cgtataggtg tagagccagt ctgaggcggt atcttcgaac
     1261 acgataaggt tgttcccatc attggtagta gctgcataac tgatcatgtc gcagttcaac
     1321 acgccaatga tattccatcc ttgatcagct gcctccgaag cgtatacttc acttcctagc
     1381 attccttgtt cttctccgga gaatgccacg aacttgatgg tatggttgaa ttgatattgg
     1441 ctcatgatcg atgctgcgat cagtaccgca gctgtccctg aggtgtcatc atcagcccct
     1501 ggacccgcag agaccgtgtc ataatgggca cagatgatgt agatttcatc gcttgtttca
     1561 tctgttccat tcagtgtcgc ttcgacatta tctgatatgt accctccatt gctccagtgg
     1621 tgataccgga ctgcaagacc aaagctttgg aattggtcgt acatgtactc ggctgctgcg
     1681 atacaggaag gactttctgt cggccgtggt ccgttggcag tgagattttc ctcataactt
     1741 aagaaaatag attcgtcaac ctgctggatg atgctgatga cgagatcatc catggtcgcc
     1801 gttgtttttt caggatgctc acgaatgatt ggtgcgtctg ttattgtgct aagtgatggt
     1861 gttggaacat acgctggacc gggtggttga attcgtggtt gcgtgatatt aaaattcttt
     1921 gtttctcctg tttggattga aagaccaacg acagggaaga cagtcacgat cataagcata
     1981 catacaacaa gacaaatgat tttactcttc atcataaggt ttttccccaa ctatactata
     2041 cctgacatag tatttaaatt ttcatagcaa aatactgttt ttttatgtgt ttattcctcc
     2101 tatgtcaaaa acaaattacc tgtttatcca agaatgcaaa atgtacaatc ataagaaatg
     2161 ctcctggact aaaaaaattt cctgattttc catcgcgtcg gcggggagtg ttctaagcta
     2221 tgtgcgatga cgtccgcgct ggttttcagc acgaagagca cgaggtatcc gaccggttcc
     2281 agctgtgcat cacggctaag atacaatgag gcaatgatga tgccagcgaa cagaaatgcg
     2341 acgaagagag gcagcatcct gagatatggt cggagaatag cgaatccgag attcttgtgg
     2401 accacccgtt ctctcttttt attgtagatg taggaaaaca gatgattggt gaagaacagc
     2461 agggaggtga acagcagaat agttccattg acgttggtta ccggcccaat gaccagccaa
     2521 agcacgaagg tcaggcacaa ctggacgacc gtgaagtggg caaggaaaat agtccctgtc
     2581 cgtagcttcc cctttagagt gaatcgaaga cgaatccgtt cgactcccgt caaggacaga
     2641 tctgtaagcg cgatggtacg caagaacgca aagatgccaa tgatgaggat ttgacaccag
     2701 tacacccaga tgatcgtgga gaacgaccac tgctcgagga atgcagccga tatggtaacc
     2761 atgttggtaa ggacaagtga ccaggcagaa aggtctgtcc agacatgtct gagaagacgt
     2821 tgaaccctgc atttcgtaac atcaagaagg gttacgacgc gacgcaatgc tggttttcgt
     2881 acgtaacgag agcgcatacc tgttcatcca tggattcgac attgtttttg gagttcataa
     2941 ggataagctt ttttaaaaag cctttacgat ggtacgaaaa atctccctga cttaaaaaga
     3001 tgatacgatg agattcggtt tagtatccat cgtcgtttcc gttgtgttgt ttacatttgg
     3061 tttttatcgt cttcttaaag agaagataag aaactgaagg atgtttgcgt tcagaggttt
     3121 tttagatact tcactgcttc tttttgagtg agtttcttta cgttttttcc ctgtttcatc
     3181 tctccttcga tgtgtttcat cgcgaggtac tgttcctttg tcatggaagc ggtgcgaaga
     3241 caggagttga tgatgacgtt ttgttgtcga gaccagtctt ggtcgctatg ggtccggacg
     3301 tagtctgccc attggtcgat gaaacgattt cgttcttttt ggttttcctt agtgaacgtc
     3361 acggagcatc tccttgtagt gtttaagcag ttttttatct aaatgccctt ctgctacttt
     3421 ttggatgtgg cgtgcgtctt ctctgtcttt tggtgatctt aggacctttt ctttgaatgc
     3481 gatctgcagt tcgagattcg agatatgtag ggtcccttgt ggaagtatga cggtgattgt
     3541 cttttgcaat gcaagaccct ggatgcggtt ttttgccgat ttcagctcga tgttggggat
     3601 catgattttt tgttttgcga atctgactgt gcgaccttcg gtgagacatg catagatgtc
     3661 gctgcctttt tcttcattaa tggaatagaa tcctttctct tttaggtcgt gtactaattt
     3721 ttttaatgta ggggatggga tctttgggat gatgatgtcg atatcctcac ttgcgcgcgc
     3781 tcttcctaga agaatagaga cgtacccgct gacaatcaca tagagggtgt gtttctcaag
     3841 aacggtggta aaatcaagtg ctaatcggtc gagatctgag ggttctctgt tgatctcaag
     3901 ggttgttgtg tcgatgaatc tcattgtttc tctctggtac gaatgatgtt ctactatatc
     3961 attttttatc ggggttttca taaagaggaa ttttaccgat gtatttctca catttttatg
     4021 attgcaactt gttataaatt tttattaaat cgttggctag ctgcttttcc ccaaggttgt
     4081 ttgccatatc gatgttgttg aacgtatatt tagataggtc tggggatgtt acttgtagtt
     4141 tttcaaataa caaagc
//
LOCUS       DATMXJ010000269         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_30762, whole genome shotgun sequence.
ACCESSION   DATMXJ010000269 DATMXJ010000000
VERSION     DATMXJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30762"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2901
                     /locus_tag="VN377_07000"
     CDS             <1..2901
                     /locus_tag="VN377_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWR28165.1"
                     /translation="RIDAELSYSVKNLYTDGIDDAVEVANSNTISVTNECTLEAWVNR
                     SGYSDGLVGLPLQLSLSQFGNYTLGCVDPCLLHVNGDIFALVSTNENSYGFLSTINIS
                     DTGIIYQNVSTSSCILDFFNFDSSCKNPKIIQVSGINGIYAIVYTRPGSGNQLYIKTV
                     QIFDDGHINKIPIGTRVLDTNISASPDIIKIDFNTYAIVYSITAVNNGVLISLDISFA
                     GVISPVNKRLLFGDIMIEPEIIKIVDSFDKYVIVYNCIGDDGGIRTVKITNGGLLSDI
                     SYHVWYDDDDGGSPEITHVHDDVYAIVYAGPIVRQSCILKTIEILPDGSITLSRTIPP
                     LAGAISQQSIDFIANMTVRSPHILPLFGMNDFYGISYSVDSTTANLWGKIVTVMIQHN
                     GNIVGISKKDVTFEPFTCSQSYFIPIANELYGIVYRSESNDGIIKAIEIGNDGHIHNN
                     PILDMVGIGGLKCYVEDEVLTSDNQYVVDVYRGINAQLIVKTVKVNTSSKTIAQTFTD
                     SMIIERGYTSSNGTFNASYAPTIIPIKNDVYAIAYCHYMTVPMYHHGRIVTIRINATG
                     RISLIERYTFDNNVMNNPILFTPINKTNGFYAVAYQLYSTSQGKITTIKIGDNGHIFG
                     VQDSYIFESGRCREPSMTQVSGDIYALMYRDSLTSSTYGRLATLKIYGNNGTIKKSVI
                     DLWQFATSCYHPTIIKVNSTTFACVFSTYDGGAGRYIAYVTTVKIASNGIISKTWIDY
                     LEFIRRYYTDNYMAHQPEIFHVDNRVYAIISKDLPDPWNNYVYNSYITTLRIGENGDI
                     IDAVDGSVQISTSPRITSYDMKIIPFVNDCYIAIYGGKNYDLYQCVVRIPLTGTNQTI
                     FSKQDSYTIKANKTKVFVTFTDSNNNQYTLSGDLSSNWNYIVSTYDRTTMNLYLNTNL
                     IGSRALGSKPIKITTNKLYFGPYNSYCDEFSLYAAILSQAKITQNYNYNRPS"
     gene            2931..3797
                     /locus_tag="VN377_07005"
     CDS             2931..3797
                     /locus_tag="VN377_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28166.1"
                     /translation="MRKIIKSIRKKNEAVAGIVVAVMIVGLVLAVISIIQTIYIPKWM
                     ESREAEHMGVVSDQFSQLKFTVDTQIALKENIPVSSSITLGSRELGFFMSNKAFGRLA
                     LISNGWAHRIVRTAGDTYEDNFGILRYTSENAYFLNQVYNYEVGGVVLNQTEGAVFVI
                     KPEFSARYNASTRQVNLSLTCIDLVPNDEKTSISGYGTYPVRTQYISATNTTITSIKT
                     LAIITPFSSIWYNFLNSTLSDANLVKNTDYTMTKTSNQVTVTFNSPPLTNVNLDLTKI
                     QIAAQITPGWSD"
     gene            complement(3916..>5210)
                     /locus_tag="VN377_07010"
     CDS             complement(3916..>5210)
                     /locus_tag="VN377_07010"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HWR28167.1"
                     /translation="NILKEAKEKMKRKNILKAALVLSIALAMIMPASAIFTDKQPTNS
                     KLGTTYSTQTQQISKPKAIGSYDDILSEGFETAWVADSDGDLAPPGWEVHQTCTSGTD
                     PFQGYWHRTGTIPFSSGSVSPHGGSWQAFVQWSYDHQDEWLVTPLLPLGAGSNLEFWI
                     YGHYGSVNLDHYYVKLSPSGGFNPSDFTVVLWDATALPAGDNFYDTPVEIDLSAYDGQ
                     NVRLAWNFVDGDGAGLWYATVIDDVLVTSGGGGGDTTPPVTTYNITGTNPVTITLTAT
                     DDDSGVNHTYYKIDAGTYAEYTAPVQVTEAGDHIVYFYSVDKAGNTETEKSKAFTVEA
                     PPITITIKGGFGVSVTIKNTGASDLTDIDWTIDLDGKLIFVGKNRSNTIDALAAGAEV
                     TVKDFVIGFGKTGIAATAGTATASASGTALLFFVIGVA"
BASE COUNT         1592 a         1088 c         1042 g         1488 t
ORIGIN      
        1 agaatagatg cagaattgag ttattcagta aagaatttgt atacggatgg aatcgatgac
       61 gctgttgagg tagcgaattc aaacacgatc agcgtcacca atgaatgcac ccttgaagca
      121 tgggttaatc gttcaggata cagtgatggg cttgttgggc tccctcttca attatcgtta
      181 tcacaattcg gtaactatac tttaggatgt gttgatcctt gtcttctcca tgtcaatgga
      241 gacatcttcg ctcttgtctc aacaaatgaa aactcctatg gttttctgag cactataaat
      301 ataagtgata cgggaattat ttatcaaaat gtatcgacat cttcttgtat tttggatttt
      361 tttaattttg attcctcctg taaaaatcca aaaattatcc aagtaagcgg aatcaatgga
      421 atttatgcaa tagtatatac cagaccaggc tctggaaacc aattatacat aaagacagta
      481 caaatttttg atgatggaca tatcaataaa attcctattg gcacccgtgt tcttgatact
      541 aatatttcag caagcccaga cattataaaa atagacttta atacctatgc catagtctat
      601 tcaatcactg cagttaataa cggagtatta atctccttgg atatctcctt tgctggagta
      661 atctctcctg ttaataaaag attacttttt ggggatatca tgatagaacc ggaaatcatc
      721 aagattgttg atagttttga taagtacgtc atcgtgtata attgcatcgg ggatgatggt
      781 ggaattcgta ccgtaaaaat aaccaatggt gggctcttat ctgatatatc atatcatgtg
      841 tggtatgatg atgacgatgg tgggagtccg gagattaccc atgtccatga cgatgtctat
      901 gctattgtgt atgcagggcc gattgtaaga caatcttgca tattaaaaac gattgaaata
      961 cttcctgatg gttcaatcac attaagtcgg acaataccac cactagcggg tgctatcagt
     1021 caacaatcaa ttgattttat tgcgaatatg actgttcgtt ctccgcacat tctcccttta
     1081 tttggaatga atgacttcta tggcatctcc tacagtgtcg actccactac tgcgaatctc
     1141 tggggaaaaa tagtcaccgt catgattcaa cacaatggaa atattgtagg gatatctaag
     1201 aaggatgtga ccttcgaacc attcacatgc tcccagtcat atttcatccc tatagcaaat
     1261 gagctgtacg gaattgtcta ccgaagtgaa tccaatgatg gtatcattaa ggcaatagag
     1321 attggaaacg atggacatat ccataataat cccattcttg acatggtggg aatcggtggt
     1381 ctgaaatgct atgtggaaga tgaggttctc acttcagaca atcaatatgt tgtggatgtt
     1441 tatagaggta tcaatgccca actgatagta aaaactgtaa aagtgaacac ttcgtcaaaa
     1501 accatagcac aaacgtttac tgattcgatg attatcgagc gaggttatac ctcttcgaac
     1561 gggacattta atgcatctta tgcaccaact attatcccta ttaagaatga tgtctatgcg
     1621 atagcttatt gtcattatat gaccgtccct atgtatcatc atgggagaat tgtcacaata
     1681 agaataaacg caacaggacg tatttcgctc attgagcgat atacctttga taacaacgtc
     1741 atgaacaacc ctatcttgtt taccccaatc aacaaaacaa acggttttta tgcggttgcc
     1801 tatcaactct atagcacatc acagggaaaa ataacaacga taaaaattgg tgacaacgga
     1861 cacatttttg gtgtccaaga ctcatatatc ttcgagtctg gtcgctgccg agaaccatcc
     1921 atgactcagg tcagcggaga catatatgcc ctgatgtacc gagactctct gacatcgagt
     1981 acctacggac ggttagccac tctgaaaatc tatggcaaca atgggacgat aaaaaaaagt
     2041 gttatcgatc tctggcaatt tgcgacttca tgctatcatc caactatcat aaaagtgaat
     2101 tcaaccactt ttgcctgtgt attttctacc tatgatggtg gtgcggggcg atatattgct
     2161 tatgtcacaa ccgtaaaaat agcgagcaat ggtattatca gtaaaacttg gattgactac
     2221 ctagaattta tcagaaggta ttacactgac aattatatgg ctcaccaacc agaaatattt
     2281 catgtagata accgtgtcta tgccatcatc tcaaaggacc tccccgaccc atggaataat
     2341 tatgtgtaca atagctatat caccaccttg cggattggtg aaaatggcga tatcattgat
     2401 gcggtcgatg gttccgtaca aatttcaaca agccccagaa taaccagcta tgatatgaaa
     2461 atcataccgt tcgtcaatga ctgttacatc gctatctacg gcggtaaaaa ttatgattta
     2521 tatcaatgcg tagttcgcat tcctctcacg ggaacaaacc aaacgatttt ttcaaaacaa
     2581 gattcctata ccattaaagc aaataaaaca aaggtttttg tcacattcac cgattcgaat
     2641 aacaatcaat atacactttc aggagacctc tcaagcaact ggaactacat tgtaagcact
     2701 tatgatagaa ccacgatgaa cctgtatcta aataccaacc tcatcggttc acgagcactt
     2761 ggttcaaaac caataaaaat cacaacaaat aaactctatt tcggaccgta caacagctac
     2821 tgtgatgaat tctccttgta tgctgcgata ctgtctcaag caaaaataac tcaaaattac
     2881 aattacaatc gtccatcata aaaaaaaata aaattgatag cgtgaaacct atgcgaaaaa
     2941 taataaaaag catcagaaaa aagaatgaag cagttgctgg tatcgttgtc gcagtcatga
     3001 ttgtcggact tgttctagca gtgatttcaa ttatccaaac gatctatatt ccaaagtgga
     3061 tggaatcacg agaggccgaa catatgggtg tcgtgtcaga tcagttttca caactcaaat
     3121 ttacggttga cacgcaaata gctcttaagg aaaacatacc agtatcctcc tcaatcacct
     3181 taggcagtcg agagctggga tttttcatgt caaataaagc atttggtcgg ctcgcattga
     3241 tctctaatgg ttgggctcat agaatcgtac ggaccgcagg ggatacctat gaggataatt
     3301 tcgggatcct tcgctataca tcggaaaatg cgtatttcct caatcaagtc tataactatg
     3361 aggtgggggg agtagtgctc aatcaaaccg aaggagcagt ctttgtcatc aaaccggagt
     3421 tctcagcccg ttataacgct tccacacgcc aagtaaacct ctcattgacc tgtatcgatc
     3481 ttgtccctaa cgatgaaaaa acatccatca gcggctacgg aacctatccg gtacgcacac
     3541 aatatatcag cgcaacgaac acgactatca cctcaattaa aaccttagcg atcatcacac
     3601 cattttcatc catctggtat aattttttaa attcaacgtt atctgatgca aatcttgtaa
     3661 aaaacactga ttacaccatg acaaaaacat cgaatcaagt gacagtaacc tttaattctc
     3721 ctcccttgac aaacgtaaat ctggatctta cgaaaataca gattgctgct cagataacac
     3781 caggatggtc agattaaaac tctagaaaaa acgaatcatc cgatcatctg tctctcagaa
     3841 ggttatagaa cagaagcagt attcatactt ttttttaaaa agataagatt aaaaaaagaa
     3901 aaagaggggg ggagtctatg cgactccgat gacgaagaac aacagggctg tgcctgatgc
     3961 gctcgccgta gcagttcctg ctgtggctgc gatgccggtt ttaccaaaac cgattacgaa
     4021 gtctttcacg gtgacttcag ctccggctgc aagggcgtca atcgtatttg atctgttttt
     4081 tccgacgaag atgagtttcc catcaaggtc aatggtccag tcgatgtcgg tcagatcaga
     4141 agccccggtg tttttaatcg ttactgagac gccaaacccg cctttaatcg ttatcgtgat
     4201 cggtggtgct tcgacggtga aggctttgct tttctctgtc tctgtgttac ctgctttatc
     4261 aacggagtag aaatagacga tgtgatcgcc agcttcagtg acttgaacag gtgcggtgta
     4321 ttctgcatag gtgccagcgt cgattttgta ataggtgtgg ttgacaccgg agtcgtcatc
     4381 ggtggctgtt aacgtgatgg tgactgggtt tgtcccggtg atgttgtagg tcgtcaccgg
     4441 tggagtggta tctcctccgc cgccgccaga agtaacaaga acatcatcga taacagttgc
     4501 ataccaaaga cctgcaccat cgccatcaac aaaattccat gcaaggcgga cgttctgtcc
     4561 gtcatatgca gaaaggtcga tttcaaccgg ggtatcataa aaattatccc cagcaggcag
     4621 tgcagttgca tcccaaagta ccacggtgaa atcagatggg ttaaaacctc ccgatggcga
     4681 aagcttcaca tagtaatgat cgagattaac cgaaccgtaa tggccataga tccagaattc
     4741 caaattggag ccggccccca gtggtagcag tggtgttacg agccattcat cttgatgatc
     4801 gtaactccat tggacgaatg cttgccaaga cccgccatgt ggtgatactg aacctgatga
     4861 gaatggaatg gtaccagttc gatgccaata tccctggaaa ggatctgttc cactcgtaca
     4921 tgtctgatga acttcccaac caggtggtgc aagatccccg tcactatcag cgacccatgc
     4981 tgtttcaaaa ccttcgctga gaatgtcatc ataagaacca atggcttttg gttttgatat
     5041 ttgttgggtt tgtgtcgaat aagttgtacc aagttttgaa ttggttggtt gtttgtctgt
     5101 aaatattgcc gatgcaggca ttatcattgc aagggcgatg cttagaacta aagctgcctt
     5161 taagatattt tttcttttca ttttttcttt tgcctccttt aatatattag
//
LOCUS       DATMXJ010000270         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_10187, whole genome shotgun sequence.
ACCESSION   DATMXJ010000270 DATMXJ010000000
VERSION     DATMXJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10187"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..2314
                     /locus_tag="VN377_07015"
     CDS             116..2314
                     /locus_tag="VN377_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011406942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HWR28168.1"
                     /translation="MGRKEDNIQKATALMNKLDHIRNIGIAAHIDHGKTTFSDNLLAG
                     CGMMSEELAGKQLVLDYDEQEQARGITINAASASMVHNVEGQDYLINLIDTPGHVDFG
                     GDVTRAMRAVDGCIIVVCAVEGAMPQTETVIRQAIREKVKPVLFINKVDRLINELRVT
                     PEEMQNRFIKIINKFNELLDRVVGEDFKGKWNVKVEDGSVAFGSAFHNWAVSVPRMKK
                     TNLGFKDVYNYCKENDQKSLSKRAPIHEVVLDMVIDHLPPPNVAQKYRIPHIWRGDLT
                     SPLGKNMVECNENGPLAMMVTKIILDPHAGEVAIGRMFSGKIQRGQELYVIGMPNKNR
                     VQQTNVMVGPDRIPIDYVSAGNIVAATGIRDAIAGCTVTDDPNAEPFERIVHVSEPVV
                     TVAVEAKNTKDLPKLVEVLRLISKADPSIQVQINQETGENLMSGMGELHLEITEYRIR
                     NDHKMEIVCSEPIVVYRETVSKPSPHKFEGKSPNKHNRFYIEVEPLPENIVKAIYDNE
                     LPANVKKYKKETAKILQDLGMDKDAAKGVFGVHGLNIITDTTKGIQHLFETRDLIMEA
                     FDEVMKSGPTVSEPCQGILVKLVDAKLHEDAIHRGPAQVIPAARNAIYGAITISEPTL
                     LEPMQKVFISTPQEIMGDASRELNSRRSEVKDIKTVGDSVEIEAKAPVAEMFGFASAI
                     RSATGGRVLWNTENMGFEPLPRQLKDPVIKKIRERKGLKPEPYPADYYMG"
     gene            2358..2732
                     /locus_tag="VN377_07020"
     CDS             2358..2732
                     /locus_tag="VN377_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28169.1"
                     /translation="MDTVATMQRGIIPINQFFELEYRYHEKDLNYKYFNRRFEIYLIG
                     KKGVQKIYILHMDNCDIRPGKWAPHIHRASNVAKKLYFGVSTLNWNEIKENFLSAIIA
                     EIGDTYKADAKKAVVNLLSPKL"
     gene            complement(2979..5087)
                     /locus_tag="VN377_07025"
     CDS             complement(2979..5087)
                     /locus_tag="VN377_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004515948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HWR28170.1"
                     /translation="MVRLLKDESLTASWQGFFEDQCKAEIETLATEYPEKRSLLLDYW
                     DVDKADPKLADLLLHQPYKSLFNAEEALRLIDVAAEQKLQLHFRVKNIPESQKVLIRK
                     IRANRLGTFIAVEGLVKKITEVRPKLQVAAFQCQKCGAVMRIEQDEDILKEPSECYED
                     QGGCGRVSSFKLSTTLSSFIDSQKIEIQENPEGLRGGAQPERISVYLEDDLVGNVAPG
                     DRVIVNGILHSMARHRGTFRLTSFDKTMDAVSIESQEYAFEEVEVSKEDEKKILKISK
                     DPLIYDKMRQSIAPTIYGLEIEKDALVLQLFGGIAKEMPDGTRTRGDIHALLVGDPGT
                     AKSQMLSYMSKLAPRSVYASGKASSAAGLTAAAVRDEFGEGQWTLEAGALVLADKGLA
                     CIDEIDKMEETDRSSLHQAMEQQEISIAKAGINATLKSRCAVLAAANPKLGRFDEFIP
                     MHEQINMPPALLSRFDLIFSILDKPNRKTDTELATHILQSHKGGEIHEHLNKTSDELY
                     SKEQETKFMKHVLPVFEPEFLRKYVAFAKRNVFPVMTDDALEILQNYYVDFRSSSKDS
                     VPFTPRQLEAFVRLAESSARIRLSQEATVDDAKRAISIIDQYLRRVGTDRETGRFDID
                     IIATGISHSQHERMRVILDVIQRLCTESTDDSAARSDIINEAEIQGIESRKVEEVLDR
                     LNRNGQIYEPSHGKYRLAGQ"
     gene            complement(5140..5454)
                     /gene="ffs"
                     /locus_tag="VN377_07030"
     ncRNA           complement(5140..5454)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN377_07030"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(5468..5746)
                     /locus_tag="VN377_07035"
     CDS             complement(5468..5746)
                     /locus_tag="VN377_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28171.1"
                     /translation="MSENPILQVDKKTWNKWSFYINVIIFIIIAVVIYLLILDGFNAG
                     TVYAQNNATLLTNAWIAVVRDVAFLAVGLVILFVQMFNYYRQLSRRSW"
     gene            complement(5809..>6644)
                     /locus_tag="VN377_07040"
     CDS             complement(5809..>6644)
                     /locus_tag="VN377_07040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033195.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HWR28172.1"
                     /translation="STYCFDIEGENIEDKTVLWHNQLLQIRCDGDEDGLMLLMDALLN
                     FSHAYIPDKRGGKMDLPLILTTRLDPSEVDKEAHNLDTLSRYPLEFYEATLRHEHSKN
                     LESKMGLVASRLGTELQYEQFGFTHDTDNISEGPKESLYKTLKSMMEKMNVQLSLAAK
                     IRAVDEADVAYKVIERHFLPDILGNLRAFSKQSVRCPLCNTTYRRIPLQGICTKCNGK
                     LTLTVHEMSVKKYLNISKEIAEKYNLPVYAKQRIALVEKSIDSTFMSDKVKNTKLTDF
                     F"
BASE COUNT         1867 a         1617 c         1471 g         1689 t
ORIGIN      
        1 aggctagcaa gaatgatcct gcatcttttg cagttgcaaa gaaaaatgat cttgagcggg
       61 tcgccaaagc agcacgataa aaaaattgat atggattttt gaaggtgtat aacatatggg
      121 gcgaaaagaa gataatattc agaaagcaac agcgctgatg aataaattag atcatattcg
      181 aaacattggg attgctgctc atattgatca tggcaaaacc acgttttctg ataaccttct
      241 tgcaggttgc ggtatgatga gcgaggaact tgcaggaaaa caactggtac tggactacga
      301 tgagcaagag caggcacgag ggattacgat taacgcagca tcggcatcca tggttcacaa
      361 tgttgaagga caagattact tgattaactt aattgataca ccagggcatg ttgacttcgg
      421 tggtgatgtc acccgtgcga tgcgagcagt tgatggctgt atcattgtag tctgcgctgt
      481 tgaaggcgcg atgccacaaa ccgagacggt gatacgtcaa gcgatccgtg aaaaagtcaa
      541 accagtcctg ttcatcaaca aagtagaccg actcattaac gagcttcgag tcacccctga
      601 agagatgcaa aatcgattca ttaaaatcat taataaattc aatgaactcc ttgatcgagt
      661 agtcggtgaa gacttcaaag gaaaatggaa cgtgaaggtc gaagacggta gtgtcgcgtt
      721 cggttccgcg ttccataact gggcggtctc cgttccccgt atgaaaaaaa ccaacttagg
      781 attcaaagat gtgtataact actgtaaaga aaatgatcag aaatccttat cgaaacgagc
      841 ccccatccat gaagttgtcc tggacatggt tattgatcat cttccacctc cgaatgtcgc
      901 acagaaatat aggattccac acatctggag aggagatctc acaagtccgc ttggcaaaaa
      961 catggtcgaa tgtaacgaga acgggccgct cgcgatgatg gttacgaaga tcatccttga
     1021 cccccacgca ggtgaggtag cgataggtcg gatgttcagt ggaaagattc agcgtggaca
     1081 ggagttatat gtcattggaa tgcctaacaa gaatcgtgtt caacagacca atgtcatggt
     1141 aggacctgat cgtatcccta ttgactatgt aagcgcagga aatattgttg ctgcaacagg
     1201 tatccgtgat gccattgccg gatgtactgt gaccgatgac cctaacgctg agccgtttga
     1261 acggattgtt catgtctcag aaccagttgt caccgtagcg gtcgaagcga aaaacacaaa
     1321 agacctccct aaactcgttg aggtattacg attaatctcc aaagcagatc ccagcatcca
     1381 agtgcagatc aaccaggaga ccggggagaa cctgatgtcc ggtatgggag aattacatct
     1441 agagattacc gagtatcgta tccgcaatga ccataaaatg gaaattgtct gctctgaacc
     1501 cattgtggtc tatcgtgaga ctgtcagcaa accaagtccc cataaatttg aagggaaatc
     1561 accgaacaaa cacaacaggt tctacatcga ggtagaaccc ctcccggaga acatcgtcaa
     1621 agcgatttac gataatgaat tgccagcgaa cgtgaagaaa tacaagaaag aaacagcaaa
     1681 aatcctacag gaccttggga tggacaaaga tgccgccaaa ggcgtgttcg gcgtacatgg
     1741 tcttaacata atcaccgata ccaccaaggg tatccagcat cttttcgaga caagggatct
     1801 gattatggaa gcctttgacg aagtaatgaa aagcggacct accgtctcag aaccctgtca
     1861 agggatattg gtaaaactcg ttgatgcaaa actccatgag gacgcaatcc accgaggacc
     1921 agcacaggtc atcccagcag ccagaaacgc tatctatggt gctattacga tttccgagcc
     1981 taccctgctt gagccaatgc agaaagtatt tatcagcacc cctcaggaaa tcatgggtga
     2041 cgcgtcacgg gaactgaact cccgacgctc cgaagtcaaa gacataaaaa ccgttggtga
     2101 ctccgtggaa atcgaggcaa aagcacctgt tgctgagatg ttcgggttcg catccgctat
     2161 acgatctgca accggcgggc gagtcttatg gaatactgag aatatgggtt ttgagccctt
     2221 accgcgacag ctgaaagacc ccgtgatcaa aaagatccgt gagcgcaaag gcctcaaacc
     2281 agagccatac ccagctgatt actacatggg ataaaaccct ctgtagagga atatttccaa
     2341 attcttaaaa accatgtgtg gatacagtgg cgactatgca gcgaggaatc atcccgatca
     2401 accagttttt cgagttggaa taccgatatc acgagaaaga tttgaactac aagtatttta
     2461 acagacgttt cgaaatatac ttgattggaa aaaaaggagt ccagaagatc tacattttac
     2521 atatggataa ttgcgatata cgaccaggga aatgggctcc gcatattcac cgggcatcga
     2581 acgtggcaaa gaaactctat ttcggggtct cgacactcaa ctggaatgaa ataaaagaaa
     2641 atttcctctc cgccatcatc gcagagatcg gtgatacgta caaagcagat gcgaaaaaag
     2701 ccgtcgtcaa cctcctctcg ccaaaactat aaaaaaaatt gtcttcttct tgcattgttt
     2761 caaatatgtt tttatactag ctggttccat tcatactcta tggaaatggt tccgcttcct
     2821 gaggttgagg aaacattaat cgcctttaaa aagaaaaaac aaaaacagaa aaagaaagaa
     2881 gaagaacaag aggaagaacc aaaggataaa taagagaaat tcttctgttc atcgtttttt
     2941 actagtgaca agagattgaa ctattatcgg gttcatcgtc attgtccagc gagtcgatat
     3001 ttcccatggc ttggctcata gatctgtccg ttacggttca atcgatcaag gacctcctcg
     3061 accttccgcg actcgattcc ctgaatctcc gcttcgttga taatatcgct tcgcgcagca
     3121 ctgtcatcag tagattccgt gcagagacgc tgaataacat caagaatcac cctcatacgc
     3181 tcatgctgac tatgactaat accggtcgca atgatatcaa tatcaaaacg ccctgtctcg
     3241 cggtccgtcc cgactcgacg cagatactga tcgataatcg agatcgcccg tttcgcgtca
     3301 tcgaccgttg cctcttggct caggcgtatc cgagcgcttg attcagcaag tcgcacaaac
     3361 gcctcaagct gtcgtggggt gaacggcacc gagtcttttg aggagctccg gaaatcgacg
     3421 tagtaattct gcagaatctc aagggcatca tccgtcatca ccgggaacac attccgcttc
     3481 gcaaacgcga cgtacttccg caaaaactcc ggttcaaaca ccggcagcac atgtttcatg
     3541 aattttgttt cctgctcctt cgaatacagc tcatcagagg ttttattgag atgctcatgg
     3601 atctctcctc ctttgtggga ttgcaggata tgcgtcgcaa gctccgtatc tgtcttccga
     3661 ttcggtttat caagaatgga aaagataagg tcaaaacgcg acaacaacgc gggtggcatg
     3721 ttgatttgtt catgcatcgg tataaactca tcaaaacgcc ccagttttgg gtttgctgca
     3781 gctaggactg cacagcgtga cttcagagtc gcgtttatcc ctgccttcgc gatggaaatc
     3841 tcctgctgct ccatcgcctg atgcagactg ctccggtctg tttcctccat tttgtcgatt
     3901 tcatcgatac aggcaagacc tttatcagca agcacgagcg cgccagcttc aagagtccat
     3961 tgtccttcac cgaactcgtc acgaaccgcg gcagcagtaa gaccagccgc agaggatgcc
     4021 ttccccgatg cgtacacgct tcgaggggca agcttcgaca tatatgacaa catctgcgat
     4081 tttgcagtac cagggtctcc tacgagtaac gcatggatat ctccacgtgt tctcgtccca
     4141 tcaggcattt ctttggctat ccctccaaag agttgaagga cgagagcgtc tttttcgatt
     4201 tcaagcccat agattgtcgg tgcgatactc tgccgcatct tgtcataaat caacgggtct
     4261 ttacttattt tcaggatctt tttttcatcc tctttagata cctcaacttc ttcaaacgca
     4321 tactcctggc tttcaatact gaccgcatcc attgttttat caaacgaggt aagccggaat
     4381 gtcccccggt gacgagccat cgaatgaaga ataccattca caatcacacg atctcctggt
     4441 gcgacattcc ccacaagatc atcctcgaga tacacgctga tccgctcagg ctgtgcacca
     4501 ccacgcaacc cctcagggtt ctcctggatc tctatcttct gagagtcaat aaacgacgaa
     4561 agcgtcgttg acagtttaaa ggatgatacc cgtccacatc caccttgatc ctcataacac
     4621 tcagacggtt ccttgaggat atcctcatcc tgttctatcc gcatcaccgc accgcacttc
     4681 tgacactgga acgcggcgac ctgcagcttt ggccgtacct ccgtgatttt cttcacaaga
     4741 ccctcgactg cgataaatgt ccctagccgg ttcgcccgga tcttccgaat taacaccttt
     4801 tgagactccg gaatattctt tacacgaaaa tgaagctgca gtttctgctc tgcagcaaca
     4861 tcaataagcc ggagtgcctc ctccgcgtta aaaagagatt tatagggctg atgaagcagg
     4921 agatcagcaa gcttcggatc tgctttatcc acatcccaat aatcaagaag caaactacgt
     4981 ttctcgggat actcagtcgc tagtgtctca atctctgctt tgcattgatc ttcaaagaac
     5041 ccttgccagg atgccgtgag actctcgtct ttcaacaatc gaaccatagg aaaacctccc
     5101 atactcgtta aacatcatgc atatttaaaa ttttggcggg tgtagacgcc tcttgagtgg
     5161 gtgcgcgtga tatgcaggtg actgcccgtc tggattctca accccgttaa ccctctgagc
     5221 atcggcagtc tgtggtaagg ctgctcactt ccgtgcctca cccggttccc acagcgaaga
     5281 tagacatatg accctccggt catatgcgat tatcacctct gatcccaaag gacaaggcgt
     5341 cataggcaac aaacggatca cgagatactc gttacaccgc ctcgtaagat cgaccccagc
     5401 gtatcggcga tttctggtga cagggaacgc ctagctcccc ggtctattct acacctttcg
     5461 ccactgttta ccaggaacga cggctaagct gtcggtagta gttaaacatt tgcacgaaca
     5521 aaatgaccaa gcctaccgct agaaacgcaa catcacgtac caccgcgatc cacgcgtttg
     5581 ttaggagtgt cgcgttgttt tgtgcataga ctgttcctgc gttgaaccca tcaagaatca
     5641 gaagataaat gaccactgcg atgatgatga aaataatcac gtttatgtaa aacgaccatt
     5701 tgttccatgt ctttttatca acctggagaa ttgggttctc actcatgcac accaacctat
     5761 aacaagcatg agcagatgga tataagaata ttatgattct gtcaattgtt aaaaaaaatc
     5821 agttaatttc gtgtttttca ccttatcgct cataaacgtc gaatcaatgg atttctccac
     5881 tagagcgata cgttgtttcg cgtacactgg aaggttatat ttctcggcga tttcctttga
     5941 gatattaaga tatttcttca cagacatctc atggaccgtc agggtgagtt tcccgttgca
     6001 tttcgtgcag atgccctgta gagggatcct tcgatatgtt gtgttacata atggacaacg
     6061 caccgactgt ttgctgaacg cacgaagatt cccaaggatg tctggtagga aatgacgttc
     6121 gatgaccttg taggcgacat ccgcctcatc gacagccctg atctttgcag cgagacttaa
     6181 ttgcacgttc atcttctcca tcatgctctt caatgtctta tacaacgatt cctttggtcc
     6241 ttctgagata ttatcggtat catgggtaaa tccaaactgc tcgtactgga gctcggtgcc
     6301 gagccgggat gcgaccagcc ccatctttga ctcgaggttt ttcgaatgct catgtcgtaa
     6361 ggtcgcctcg tagaactcca atggatatcg agagagggta tcgagattat gtgcctcttt
     6421 atcgacctcg gatggatcga gtcgtgttgt taaaatcaag ggtaaatcca tttttccccc
     6481 tcgtttatct gggatgtaag catgggagaa attcagtaac gcatccatga gtaacatgag
     6541 gccatcttcg tcgccatcac atcgtatttg aaggagctgg ttatgccaaa gaaccgtttt
     6601 atcttcaatg ttttctcctt cgatatcaaa acagtatgtc gact
//
LOCUS       DATMXJ010000271         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_25645, whole genome shotgun sequence.
ACCESSION   DATMXJ010000271 DATMXJ010000000
VERSION     DATMXJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25645"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2436
                     /locus_tag="VN377_07045"
     CDS             187..2436
                     /locus_tag="VN377_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HWR28173.1"
                     /translation="MKKKITVLLVCFIVIATVVPVVGSLKDNTSYSSLPNTATNLKKD
                     PDKKIQNISKNSDVSNRNPETWEVDDDLVQCPGADFVQIQPAIDAAGDGDSIIVYDGT
                     YPENLNVNKQLSLGAAGLNVIINGLGNDDVVDVNADGVSLHGFYILNSSVGKAGVNCT
                     DYNTMTVLMCFLFQNFYGIRIHNSNGNSIIGCLSFNNFADGVNISESVSDTISNSFIF
                     GNAYNGIKIKQATNINIESNGIHNNTFEGINISSSGLVASPIIISGNRIVDNNGNGIV
                     LQNSEYVEMSGNSAISNNSKNGVLLLHSNWSDIRNTLRIYHNGVGGSSLEERSGVHLL
                     SSDNNTIRNNICDYNLGFNIGLRQGSDNNTVEGNDCGFSGDDGISIRYGSDDNKIQFQ
                     NKVYNSTDEGIHLRDEIGDGGSARNTILGNVVWNNSLGGIYCEAGVGRNNSIEQNRVY
                     HNGFDSGSWLDGISLMGSQYTIISLNFVYDNHQHGISLYQNANHNIISENYVYNTVAG
                     NQQNGIHLDSSHQNIISDCSIYNNSLHGIFIRNSNSNLIYNNYFNNTNNAEDNGNNRW
                     NISKTPGLNIIGGVFIGGNFWSNYTGFDNDGDGLGDTNYTIPGGTNTDHHPLVRANQP
                     PNKPAKPSGIVSGKINTLYTYKTSAIDPNGDQVWYQWDWGDGSQSNWLGPSNSGATIS
                     TTHKWTVKGSYSIKVKAKDTYGTESPWSDPLSIKMPYSYNYPITQFWEKLLERFPHAF
                     PILRHFLRY"
     gene            2737..>3035
                     /locus_tag="VN377_07050"
     CDS             2737..>3035
                     /locus_tag="VN377_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28174.1"
                     /translation="MTSRPKSVHIVGIVCVVISLILSCGSITGVTTSSSSLEKKEAPG
                     ALLTYSSGCCEGYTLLNRATTTVLIDMNGTIIHRWPSHYPQPAKMLPGGSIIAGS"
BASE COUNT          957 a          573 c          634 g          871 t
ORIGIN      
        1 cccttcttct ggatgaatat atagaatggt tcaacacagt gcgatattcc ctcggggtgg
       61 agggaatacc ttcatcattt tattacgaat gttagcgatg tctattgaca ttacatattc
      121 caaacattta aatgaatcta aagaataacg aaccatgacc tatgttgaca ggggggaatc
      181 tagaccatga aaaagaaaat aactgtgtta ttagtttgtt ttatagtgat agcaactgtg
      241 gttccagtag tcgggtctct gaaggataat acatcatatt catcactacc aaatactgca
      301 acgaacctga aaaaagatcc tgataaaaaa atacaaaata tttcaaaaaa tagtgatgtt
      361 agtaatagaa atcctgaaac atgggaagta gatgatgatc ttgttcaatg ccctggggcg
      421 gattttgtac aaatacaacc agcaatagat gcggcaggtg atggcgattc aatcattgtc
      481 tatgatggaa cctatcctga aaaccttaat gtgaacaaac aattatctct tggtgcagct
      541 ggattaaacg ttatcataaa tggtcttggg aacgatgatg tcgttgatgt gaatgcagat
      601 ggggtgagcc tccatggatt ttatattttg aatagtagtg tgggaaaagc aggtgtgaac
      661 tgtactgatt ataacactat gacagttctc atgtgcttcc tctttcaaaa tttttatgga
      721 attcgaatac ataacagcaa tggaaattcc atcatcggat gtctaagctt taataatttt
      781 gcagatggag tcaatatcag tgaaagtgtg agtgatacga tttccaatag cttcattttt
      841 gggaatgcgt ataatgggat caaaatcaaa caagcgacaa acatcaatat tgaaagcaat
      901 gggatacaca ataacacatt cgagggtatt aatatttcat cctccggatt ggtcgcaagc
      961 cctatcatca tctcaggaaa tcgcatcgtt gacaataatg gaaatggtat cgttcttcag
     1021 aattccgagt atgtcgagat gagcggaaat tcagcgatat ccaataatag taaaaatggt
     1081 gtgttgttgt tgcactccaa ctggtctgac attcgaaata ctctacgaat ttaccacaat
     1141 ggtgttggag ggagctccct cgaagaacga agcggtgttc atcttctctc atcagataac
     1201 aacacaattc ggaacaatat ctgtgattac aatcttggct ttaacattgg attacggcaa
     1261 ggctctgata acaacaccgt agagggaaac gattgtgggt tcagtgggga cgatggcatc
     1321 agtattcggt atggatctga tgacaataaa attcagtttc agaataaagt gtataatagc
     1381 acagatgaag ggattcatct ccgtgatgaa atcggcgatg gtggttctgc gagaaatacc
     1441 attcttggta atgtcgtatg gaacaatagt cttgggggga tttattgcga agctggtgtg
     1501 ggaagaaata attcgataga acagaatcgt gtgtaccaca atggttttga ttctggatct
     1561 tggcttgatg gtattagtct tatgggttcc cagtatacga ttatcagcct taacttcgtt
     1621 tatgataatc atcaacatgg tattagcttg tatcagaacg cgaaccataa cattatcagt
     1681 gaaaactatg tgtacaatac ggtggcagga aatcaacaga atggtattca tctcgacagc
     1741 agtcatcaaa atatcataag cgactgttcc atctacaaca attcactgca cggtatcttt
     1801 attagaaatt caaatagcaa tcttatctat aataattatt tcaacaacac gaacaacgct
     1861 gaagacaatg gcaataatcg atggaacatt tccaaaacac ctggtcttaa tatcattggt
     1921 ggggtattca tcggagggaa tttttggtct aattacaccg gtttcgacaa cgatggagat
     1981 gggctcggtg atacaaacta tacgatacct ggaggcacca atacagatca tcatccatta
     2041 gtacgtgcca accaaccacc gaacaaacca gcgaaaccat cagggattgt ttcagggaag
     2101 atcaataccc tttacaccta taaaacgagt gctatcgatc cgaatggaga ccaggtatgg
     2161 taccaatggg attggggtga cggatcccaa agtaattggc tcggtccttc caattctggt
     2221 gccacgatca gtacgactca taaatggacc gtaaaaggat cgtatagtat caaagtaaaa
     2281 gcaaaagata cgtatggtac ggaaagccca tggtctgacc cgttatctat aaagatgcca
     2341 tattcgtata actatccaat aactcaattc tgggaaaaat tgcttgagcg gttcccgcat
     2401 gcattcccga tactcagaca tttcttgaga tactaagagt cccccatctt tttctatttt
     2461 tttttatcct ttgattcgta caaagattat tgtgtcttaa aaccgtgtca atgcttctgt
     2521 cctttatttt tatcttttcc aaacatttaa atgagtccat gtgatagtat tttccaattc
     2581 accctttacc atacatatag tggttacaat caatagatag aacatgaata atagggattt
     2641 cccagaagaa accggcgcct tactaccggg tatcatgata aatcttatat cctaattaag
     2701 gttatttttt gaataaaaag aggtggaggt tccacattga catcgaggcc aaagagtgta
     2761 cacatagtag gaatcgtttg tgttgtcatc tcactcattt tatcttgtgg gagtatcaca
     2821 ggagtcacca cgtctagttc tagccttgaa aaaaaagaag ctcctggcgc tctcctcacc
     2881 tattcctcgg gatgctgtga aggctacacg ctgctgaata gagccacaac cactgtcttg
     2941 attgatatga acgggaccat catccatcga tggccgtcac attaccctca gcccgcgaag
     3001 atgctgccgg ggggatccat catcgcgggg agcaa
//
LOCUS       DATMXJ010000272         6692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Thermoplasmatota archaeon isolate
            SMAG_U13117 k141_31232, whole genome shotgun sequence.
ACCESSION   DATMXJ010000272 DATMXJ010000000
VERSION     DATMXJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943714
            Sequence Read Archive: SRR7629278
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Thermoplasmatota archaeon (soil metagenome)
  ORGANISM  Candidatus Thermoplasmatota archaeon
            Archaea; Candidatus Thermoplasmatota.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:51:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,423
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,391
            CDSs (with protein)               :: 1,391
            Genes (RNA)                       :: 29
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Candidatus Thermoplasmatota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31232"
                     /isolate="SMAG_U13117"
                     /isolation_source="soil"
                     /db_xref="taxon:2806419"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="45.02 N 93.14 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VN377_07055"
     CDS             <1..318
                     /locus_tag="VN377_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWR28175.1"
                     /translation="PLYHSVEKFHFTKDEGNTICRSNTFDLMTFRDHNITKIEDDSGN
                     KKELECNERFYVPSEITWLLKSLGFKKIDIYGAKLGAFSRNDELTTEDFEMLVIADFT
                     SEI"
     gene            complement(460..837)
                     /locus_tag="VN377_07060"
     CDS             complement(460..837)
                     /locus_tag="VN377_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HWR28176.1"
                     /translation="MVELKQVYKCALCGNIVEIIHAGTGELVCCGQPMELLTEKLQDA
                     GNEKHVPVIEKTATGVKVKVGSIPHPMEEKHYIEWIELHADESVYRKFLKPMEKPEAE
                     FCVTTKNLSARDYCNIHGLWKSK"
     gene            complement(855..1241)
                     /gene="nifU"
                     /locus_tag="VN377_07065"
     CDS             complement(855..1241)
                     /gene="nifU"
                     /locus_tag="VN377_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HWR28177.1"
                     /translation="MANVGYSKKVMEHFMNPRNVGVIENPDGYGKVGNPVCGDVMEIF
                     IKVKDDIITDIKFRTFGCGSAIATSSMVTELAKGKHIDEAVKITRGDVASELEGLPPQ
                     KMHCSNLAADALHEAIKDYKNKGQKK"
     gene            complement(1244..2413)
                     /locus_tag="VN377_07070"
     CDS             complement(1244..2413)
                     /locus_tag="VN377_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWR28178.1"
                     /translation="MPRIYLDHASSMPVDLRVFEFAKPYLTTSHGNPSSLHSVGLEAK
                     RAVEDSRKKIADLIHAENETTIIFTGGATEANNLAVKGTALRNITQGKAVVASAIEHM
                     SVLNPMKELQKNGFTFTILSVDSTGIVDLKKLEAILTKNTVLTSVMYANNEIGTIEPI
                     KEISTLVHEKGMYLHVDAVAAAGRIPIDVQKDGIDLLTLSSNDLYGPQGAGGLYIKPG
                     VKLQSVLLGGGQERGLRSGTENVFAIAGMGEAARIAQQEMAAESRRLLPIRDRLIKEI
                     LTIDQSYLTGHPAQRLPHHASFRFSYIEGESILLNMDMNDIQVSTGSACSSKTLEPSH
                     VLLAIGLKHEEAHGSMVLTLGRSTTMDDVEAVSKATNETVQRLRKLSPMSQEWRK"
     gene            complement(2426..2788)
                     /locus_tag="VN377_07075"
     CDS             complement(2426..2788)
                     /locus_tag="VN377_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWR28179.1"
                     /translation="MKDENSILYVITTDNPEKQYAPLVLAQTAKMMDLTPMVYYLGTG
                     LKILLPGAAAKIKLGSFPTVAEMLKKTMDMGITIYACEASKQMCGLEEASLMPGPKIV
                     GAGTLNDLALDAGATMWF"
     gene            complement(2798..3133)
                     /locus_tag="VN377_07080"
     CDS             complement(2798..3133)
                     /locus_tag="VN377_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWR28180.1"
                     /translation="MKAKKHQTNAPHASTRRVITNCSQRITRGDIISNEVPFDAELDC
                     VGFYCPMPIAMTKEEIEKIEIGQVLKVEADDPAAEEDIKRWAKRTGHEILKFEKEGSL
                     MTFFIKRTK"
     gene            complement(3051..3626)
                     /locus_tag="VN377_07085"
     CDS             complement(3051..3626)
                     /locus_tag="VN377_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWR28181.1"
                     /translation="MKSLKGTQTEKNLLAAFAGESQARNRYTYFASEAKKAGYEQISA
                     IFLETAENEKEHAKVYFKHLQGGMVEISAMYPAGVIGTTEENLKEAADGEKAEWGTIY
                     PEFAAMAKKEGFPEISLSFQQIAEVEEHHEKRYRALLANLVNKQVFKKEKVVKWKCRN
                     CGYVHEGKEAPNKCPACQHPQSYYELFAENY"
     gene            complement(3654..4151)
                     /locus_tag="VN377_07090"
     CDS             complement(3654..4151)
                     /locus_tag="VN377_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HWR28182.1"
                     /translation="MDLRKYTLGDLLLAAMKAEIESHTVYTTIANQVKNGLLKDKFTF
                     LATEEEKHRSFIEQVYKAKFPKKKIVVPKTTPVPLPHLAIPDEDAPLGTILKNAMAAE
                     QAAHEFYQSLSKQFTKNDAKIRNTLSYFADMELQHYKILEIEKESMDRFEEADIYWPM
                     VHAGP"
     gene            complement(4188..4682)
                     /locus_tag="VN377_07095"
     CDS             complement(4188..4682)
                     /locus_tag="VN377_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013467695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HWR28183.1"
                     /translation="MLSKKLENAINKQINAELWSAYLYLSMSAYFESINLGGFASWMR
                     VQAQEEVSHAMKFYNHIIERRGRVLVSAIAATPTSWKSPLHAFDEAFKHEQKVTAMIE
                     NLANIAAAEKDYAATSMLKWFHDEQVEEELQTDTIVHKLKMTEKSTGGLFMLDRELGK
                     RKKE"
     gene            complement(4714..4839)
                     /locus_tag="VN377_07100"
     CDS             complement(4714..4839)
                     /locus_tag="VN377_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWR28184.1"
                     /translation="MKIQYTCDCKEKNCTCDSVIGFEKEPTAVPHCCGAPMKRIK"
     gene            complement(4942..5643)
                     /locus_tag="VN377_07105"
     CDS             complement(4942..5643)
                     /locus_tag="VN377_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWR28185.1"
                     /translation="MDKKPFHKISYGIYVICSKNGEKTNGQIATTLFQVTSEPPTIAV
                     SINKQNLTHQYISESKVFTVSILDKKTQLPFIGTFGFKSGRDIDKFKNVNYKIGTTQA
                     PIVLDNTLAYLEASVINKIDVGTHTIFIGKITDAAILSEEPAMTYEYYHEVKGGYSPK
                     TAPTYDSEIDKKTEEKKEDEKMDNYVCTVCGYIYDPAKGDPETGIAAGTAFEDLPDDW
                     VCPICGAGKDAFEKQ"
     gene            complement(5636..6067)
                     /locus_tag="VN377_07110"
     CDS             complement(5636..6067)
                     /locus_tag="VN377_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWR28186.1"
                     /translation="MYEKYVKILKDHTLKITSPRLEILRYLDEHHTHPNVEEIYSTLK
                     KKNPSLSRTTVYNSLEILKKNRIVQSLTISGSELRYDIDEGLHHHFFCTHCGAIFDIA
                     IACPNMDRTVHGGHHVEEVQGYFKGICKKCQTKEKGRSNNG"
     gene            6200..>6692
                     /locus_tag="VN377_07115"
     CDS             6200..>6692
                     /locus_tag="VN377_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HWR28187.1"
                     /translation="MEYSLLYHTIIPFILSALVVILVTVIAEKYGTKTGGIVGMLPST
                     IIIAFLFIALDKGTKFASDSVAVVPAEMGFNLIFLLIFVLLSQQKILVALAGSLLGWA
                     ALTIVMYYSNLTSVVISLAIYLACFLLVFFAFDKKKKIIAQDTIKVHYTPLKLLGRSL
                     IAGI"
BASE COUNT         1505 a         1532 c         1453 g         2202 t
ORIGIN      
        1 cccttatatc attcagttga aaaatttcat tttacaaagg atgaaggaaa tactatttgt
       61 agaagtaata cttttgattt aatgactttt agagaccata atattacaaa aatagaagat
      121 gactctggta ataaaaagga attagaatgc aatgagaggt tttatgttcc atctgaaatt
      181 acatggttgt taaagtctct gggttttaaa aaaattgata tttatggtgc aaaacttggt
      241 gctttttcga gaaacgatga acttacaact gaagattttg agatgctagt tattgccgat
      301 tttacttcag aaatttgaaa aaattacgtg aattcattat tcattccgat tgaccagtaa
      361 acaccagttt aaattttttt gtttgccatt attatataaa aaatgcaggg gaagggattt
      421 gaacccgtag accgcctcaa ctggctatgg tacgattgat tatttcgatt tccacaaccc
      481 atggatattg caataatctc gtgctgagag attttttgtt gtcacacaaa attctgcttc
      541 tggtttctcc attggtttta aaaacttccg gtacaccgac tcatcagcat gcagttcgat
      601 ccattcaatg taatgcttct cctccatcgg atgaggaatc gaacctactt ttactttcac
      661 acctgtagcg gtcttttcaa taacaggcac atgtttttcg tttccagcgt cctgaagttt
      721 ttctgtgagc agctccatgg gttgaccaca acagacaagt tcgccagtac cagcgtggat
      781 gatttccacg atattcccac agagagcgca tttatagact tgttttaatt cgaccatacg
      841 tcaatgcctc catttcactt cttttgtccc ttgtttttat aatccttaat cgcctcatgc
      901 aacgcatcag cagcgagatt cgaacaatgc atcttttggg gcgggagtcc ttcgagttcg
      961 ctggcaacat caccacgagt aatcttgact gcttcatcga tgtgttttcc tttagccagc
     1021 tcagtgacca tactgctggt tgcaatagct gagccgcagc cgaacgtccg gaacttgata
     1081 tcggtgatga tatcatcttt tactttgatg aagatctcca tgacatcccc gcagaccggg
     1141 tttcctactt tcccgtaccc atcagggttt tcgatcacgc cgacgttacg tgggttcata
     1201 aaatgctcca tgactttctt actatatcct acgtttgcca ttcttatttc ctccattctt
     1261 gagacatcgg ggagagtttt cgtaagcgct ggacggtctc gttcgtcgcc ttgcttaccg
     1321 cttcaacatc atccatggta gtggaacgcc caagcgtcag caccatggac ccatgtgcct
     1381 cttcgtgttt taagcctatt gcgagaagaa catgcgacgg ttcaagtgtt tttgatgaac
     1441 aggcggagcc ggtcgacacc tgaatatcgt tcatgtccat attcaataga atgctttccc
     1501 cttctatata actaaatcgg aagcttgcat gatgcggcaa gcgttgagcg ggatgtccgg
     1561 tgagatagga ttggtcgatg gtgagaattt ccttaataag acggtctctg attggaagga
     1621 gtcgcctgct ttccgccgcc atctcttgtt gtgcgatccg tgctgcttcg cccatcccgg
     1681 cgatcgcaaa gacattctca gtcccggagc gaagaccacg ctcctggccg ccgccgagca
     1741 ggacagactg gagtttcaca ccaggtttta tatataaccc cccagcgccc tggggaccgt
     1801 ataaatcatt cgaggaaagc gtgagcaggt caataccatc cttctggaca tcgatgggaa
     1861 tgcgtccggc agcagcgact gcgtccacat gcaggtacat tcctttctca tggacaagtg
     1921 tgctgatttc tttgatcggt tcgatggtcc caatctcatt gttcgcatac atcactgacg
     1981 tcagcacggt attttttgtt agaattgcct caagcttttt gagatcgaca atccctgtcg
     2041 aatcaacaga tagtattgtg aatgtaaaac cgtttttctg cagttctttc atcggattca
     2101 gcaccgacat atgttcaata gcagaagcaa caaccgcttt gccttgggtt atgtttcgta
     2161 aagcagtgcc tttcaccgca aggttgtttg cctcagtcgc acctccggta aaaatgatgg
     2221 tggtttcgtt ctccgcgtgg ataaggtccg cgattttctt ccgtgagtct tccaccgcac
     2281 gtttcgcctc caaaccgacg gagtgaagcg aggagggatt cccatgcgac gtagtgaggt
     2341 atggttttgc gaattcgaag actcgtaggt caaccggcat cgatgatgcg tgatcaaggt
     2401 aaatccttgg catgacaatc ctcctttaga accacatggt cgccccagcg tcaagcgcaa
     2461 ggtcgtttaa ggttcctgca ccgacaattt tcgggccagg catcagagat gcttcctcta
     2521 gtccgcacat ctgtttcgat gcctcgcagg cgtagatggt aatacccatg tccatggttt
     2581 ttttcagcat ctcggcaaca gttgggaaac tgccgagttt tatttttgct gctgccccgg
     2641 gaagaaggat ttttaatcct gtcccaaggt agtataccat gggagtgaga tccatcattt
     2701 tcgcggtctg agccaggact aacggagcgt actgtttctc cgggttatcc gtggttatca
     2761 catacaatat cgagttctca tctttcatac acatacctca ttttgttcgt ttaataaaga
     2821 aggtcatcaa cgatccttct ttttcaaatt tcaatatctc atgtcctgtt cgtttcgccc
     2881 atcgtttgat gtcttcctct gcggcaggat catcagcctc gacttttaaa acctggccga
     2941 tttcgatttt ttcgatttcc tctttggtca tggcaattgg catcgggcaa tagaacccta
     3001 cacaatcaag ctctgcatca aacgggacct cattgctgat gatatcacct ctagtaattc
     3061 tctgcgaaca gttcgtaata actctgcggg tgctggcatg cggggcattt gtttggtgct
     3121 tctttgcctt catgaacata cccacagttt ctacatttcc atttcacgac tttttctttc
     3181 ttaaatacct gcttgttcac aaggtttgca agaagtgctc ggtatcgttt ctcatggtgt
     3241 tcttcgactt cagcgatttg ttgaaaactt agtgagattt caggaaatcc ttcctttttt
     3301 gccattgctg caaactctgg gtaaatcgtt ccccattctg ctttttctcc gtcggcagct
     3361 tctttgaggt tttcttctgt cgtcccgatg acgcctgcgg gatacattgc ggagatctca
     3421 accatccctc cctgtaggtg cttgaaatat accttcgcgt gttctttttc gttctccgct
     3481 gtctcaagaa agatagcgga aatttgttca taccctgctt tttttgcttc cgatgcaaaa
     3541 taagtgtaac gatttcttgc ttgtgattct cctgcgaacg ctgcgaggag atttttttct
     3601 gtttgtgttc cttttaggct tttcatcttc ttcatctcca tagttgtttc tttctaaggt
     3661 cctgcatgga ccatcggcca atagatgtca gcctcttcga atcggtccat gctttctttt
     3721 tcaatctcga ggattttgta atgctgcagc tccatgtctg cgaaatagga aagggtgttg
     3781 cgtatcttcg catcgttttt tgtgaattgt ttggagagcg attggtaaaa ctcatgggct
     3841 gcctgctctg cggccatagc gttcttcagg atcgtaccaa ggggggcgtc ttcatcaggg
     3901 atggcgagat gagggagtgg caccggtgtt gtttttggaa cgacgatttt tttcttcggg
     3961 aactttgctt tgtacacctg ttcgatgaag ctgcggtgtt tttcttcttc cgtggctagg
     4021 aatgtaaatt tgtcctttaa taacccgttt tttacttggt ttgctatagt tgtgtatacc
     4081 gtatggcttt cgatttctgc tttcatcgct gcaagcagaa ggtctccaag tgtgtatttt
     4141 ctcaaatcca tgttttcacc cttactctgt tttcctgtca gaggtgttta ttcttttttt
     4201 ctcttaccga gttctcggtc tagcatgaag agaccgccgg tgcttttttc ggtcattttt
     4261 aatttatgaa caatggtatc tgtctggagt tcctcttcga cttgttcgtc atggaaccat
     4321 ttcagcatac tggtggcagc gtagtctttt tccgctgctg cgatgtttgc aaggttctcg
     4381 atcattgcgg tgactttttg ctcgtgtttg aatgcctcat caaacgcatg taatggtgat
     4441 ttccagcttg ttggtgtcgc agcgatcgcg ctgacgagta cccggcctct gcgttcaatg
     4501 atgtggttgt agaatttcat ggcatggctg acttcctctt gtgcttggac tctcatccag
     4561 ctggcaaatc cgccaaggtt aatggattcg aagtaggcgg acatggagag atacaggtat
     4621 gctgaccaga gttctgcgtt gatttgttta ttgattgcat tttctaattt cttacttaat
     4681 atcatttttt ttacctcctt aatacacaag tttttatttg atgcgtttca tcggcgcacc
     4741 acaacaatgt gggactgctg ttggttcttt ttcaaatcca attactgagt cacaggtgca
     4801 gtttttttct ttgcagtcac atgtgtattg gatcttcata gtagaatcct cacaaccaca
     4861 gtttttacac atgagtcgaa ttcctccagt tcatgttaca attttcattt ctttatatac
     4921 tttcccggtg ctgttttttc cttattgttt ctcaaatgca tcttttcctg ctccgcagat
     4981 cggacagacc caatcatcag ggagatcctc aaacgctgtc cctgcagcaa taccggtttc
     5041 ggggtcgcct tttgcggggt cgtagatata tccacagacg gtgcatacat agttgtccat
     5101 tttttcgtcc tccttttttt cctctgtttt cttatcaatt tcgctatcat aggtcggtgc
     5161 ggttttcggt gagtacccgc ccttcacttc atgataatac tcataggtca tggctggttc
     5221 ttctgaaagg attgctgcat ctgttatttt tccgatgaag atcgtgtgcg ttcccacatc
     5281 gattttgtta atcacggatg cctcaagata ggcaagggtg ttgtccagta cgatgggtgc
     5341 ttgtgtcgtt ccgattttgt agttgacgtt tttgaatttg tcgatatccc tgcctgattt
     5401 gaacccgaac gtcccaatga aggggagctg ggttttttta tcaaggatgg atacggtgaa
     5461 caccttgctt tccgagatgt attgatgggt gagattttgt ttgttgatgc tgacagcaat
     5521 cgtgggtggt tctgaggtga cttggaataa tgtggtggcg atctgtccgt ttgttttttc
     5581 tccgttcttt gagcagatga cgtagatgcc gtaggagatt ttatggaatg gttttttatc
     5641 cattgttgct ccttcctttt tcctttgtct ggcatttctt gcagatccct ttgaagtacc
     5701 cttgtacttc ttctacgtga tggcctccat ggacggttcg gtccatatta ggacatgcga
     5761 tggcgatatc aaatatcgcg ccacagtgtg tgcagaagaa atggtggtgg agtccttcgt
     5821 cgatgtcgta tctgagttct gacccggaga tggtaagtga ctgtacgatc ctgtttttct
     5881 tgagtatttc caaggagttg tagacggtgg tacgggagag agatgggttt ttctttttca
     5941 gcgtcgaata gatttcctca acgttcggat gggtgtgatg ttcgtcgagg tatctgagga
     6001 tctcaagccg tggcgaggta atcttcagtg tgtggtcctt gaggattttt acgtattttt
     6061 catacatgcg aatcagggct gtaataaatt ccattttgta attattacaa tttagtatgt
     6121 attacaaaat aggtttatat acttttccaa aacaagcaaa aaagaaacgt ttaaaaagaa
     6181 gaacacatcc ccaagtccca tggaatactc cctcctctac cacacaatca ttcctttcat
     6241 cttatcagcc ttggtcgtca tcctcgtcac cgtcatcgca gaaaaatacg ggacaaaaac
     6301 aggaggaatc gtaggaatgc ttccatccac catcatcatc gcattcctct tcatcgccct
     6361 cgacaaagga accaagttcg cctcagattc cgtcgcagtt gtccccgcag aaatgggatt
     6421 caacctcatc ttcctgctca ttttcgtact gctttcccaa cagaaaatcc ttgtcgccct
     6481 cgctggatcg ctcctcggat gggcggctct gactatcgtg atgtattact ccaacctaac
     6541 atccgtcgtc atatctcttg ccatttacct ggcctgtttt ctcctcgtat ttttcgcctt
     6601 tgataaaaag aaaaaaataa tcgctcaaga caccatcaaa gttcactaca caccactcaa
     6661 actcctcggt cgaagcctca tcgccggtat cg
//
